LOCUS       DAOOTK010000001       103225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_168_length_103225_cov_6.586954, whole genome shotgun sequence.
ACCESSION   DAOOTK010000001 DAOOTK010000000
VERSION     DAOOTK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 103225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 103225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103225
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_103225_cov_6.586954"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PK634_00005"
     CDS             <1..1033
                     /locus_tag="PK634_00005"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOW96146.1"
                     /translation="TGPVRLDDRRMREMLTGGARWAVGQGFGSERDLERIESRGAVPG
                     ARPEDVSDRARKRQEEEMGTLGSGNHYLEVQEIRELYDEAVAAAFGLRKGEVVVSIHC
                     GSRGLGHQVATDYAKRMLDAARRHGLSLPDRELACAPIRSPEGQAYLGAMRAAINCAL
                     ANRQLLGHLVRQAFRSVFGEKEMPLLYDVSHNTCREETHEVEGAARTLFVHRKGATGA
                     LGPGHPDLPDDLRAAGQPVLVGGTMGTASWILAGARAGATRAFASACHGAGRQMSRTQ
                     ATKQWQGREVADDLAGRGVLIRCRSMRGLAEEAPGAYKDVRRVVEATVAAGLARKVAR
                     LAPLICVKG"
     gene            1148..2878
                     /gene="polX"
                     /locus_tag="PK634_00010"
     CDS             1148..2878
                     /gene="polX"
                     /locus_tag="PK634_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOW96147.1"
                     /translation="MPTHNADISAVFTEIADLLDIQGENPFRIRAYRNAARTVQDLGR
                     ELRDMVEAGEDLKKLQGIGDDLAEKIVELVQTGACTALTKLRSRFPPGVTDLLRIPGL
                     GPKRVKVLYGDLKIESLAQLEAAAREGNIRDLPGFGEKTEQNILQAIQVKADTGRRFL
                     LAHARTYAEALTAYLEKLPGAGRVTVAGSYRRGRETVGDLDILVTAESAGTIMDAFVA
                     YDEVAQVLAHGDTKSAVVLRSGIQVDLRVVEEKSYGAALHYFTGSKAHNIAVRRLGQQ
                     LDLKVNEYGVFRGEEYLAGRTEKDVYAAVGLPFIPPELREDAGEIEAAREGRLPNLVE
                     LKHLRGDLHMHTDASDGKNTLREMALAAKERGYKYIAITDHSPRLKMIGGLDGRKLAR
                     QADAIRELNNELEGIAILRGIEVDILEDGALDLPDAVLATLDLVIGSIHSHFRLPGAK
                     QTERVLRAMDRPHFTMLAHPTGRLLLDREPYELDLPRIIRHAAERGCFIELNAHPLRL
                     DLDDTFCRMAKEAGVLVSINTDAHGIPELDHLRYGIGQARRGWLEKKDVLNTRTLKQL
                     RPLLAKTMGR"
     gene            3039..3743
                     /locus_tag="PK634_00015"
     CDS             3039..3743
                     /locus_tag="PK634_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOW96148.1"
                     /translation="MTHAINSLAAVVFDFDGVIVDTEPLHFEAFRRIASPHGIELAWE
                     AYRQKYMGFDDRDAFRALFRDAGRPLAEERLAGLIDAKAAAFAKLAESEGVEPYPGAA
                     RLIGELAGRVPLALCSGALRSDVQPILARAGLKACFAVMVTAEDVSVSKPDPESYREA
                     LRRLAARFPDRTFPASRCVAIEDTPAGIAAARGAGLKVLAVRTTHAHGPLAAADCVQE
                     SLQALRVEDLEALARD"
     gene            3822..5138
                     /locus_tag="PK634_00020"
     CDS             3822..5138
                     /locus_tag="PK634_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96149.1"
                     /translation="MKKTWAWAAGIILLAGISARAQGQAGNPYYVDGMLVDQYKQSDS
                     EEAAAPVAAPNSMSLRDAPPAGPVPQPSVAPAPKAPGVALPPAGKALEHLRAANMFVE
                     KKEWDKALEEIQKGLESDPQNALLLRRGSAVAALAKNYTAADDYYRRLLMVDPNSVAF
                     MNGRASVQIRLRQFVPAQILTDRALELQPGDLLARFNRLLLKVIRNEPLEGEAWNDLL
                     TFDLAQVANWLEADKTEYQQLMSAEQFALACQTALGAGLGDKLSDVVGVLRRLGQAQR
                     LQQWDKVLAIVQEARALGLQAMGLEMDQARAVMEQGDRDQARAIMKGLSERFPEHPQV
                     LYNYGYILIEDSNYTEALGLLDRAFAQIPNHPQISFARACMLAENGRIDEAWPILTGL
                     IRDYPAETRTWLQEEDPYLAKIKADPRYQELLATRVPKRRPAGQEQ"
     gene            5155..5847
                     /locus_tag="PK634_00025"
     CDS             5155..5847
                     /locus_tag="PK634_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96150.1"
                     /translation="MKHMLIGCLCLFATAWAQAADPAISLVVVGDLDPAMAERVRAFA
                     QENLALPVRLLEPREAAAASSLNEIGDAAAEAMGPEDTCLVVLAVPTEDLPNHGVRLQ
                     EKNSAVVNARLLKPAGGDEEQYGRRLDREVMMSIGLLMGLETCPNPQCAMWLYSNDEE
                     LDMKGRNYCPPCLERIQKLAVERGLKIVADSPFAPIPDEPVPEEAASEPAAPEQAAPA
                     EAGEPEPIAETP"
     gene            5863..7128
                     /gene="serS"
                     /locus_tag="PK634_00030"
     CDS             5863..7128
                     /gene="serS"
                     /locus_tag="PK634_00030"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOW96151.1"
                     /translation="MLDIRTIREREADVRRALDLRGVGVDLDAVLALDRDRRSGLAEV
                     EQLKNQRNVISKEIGALKKAGQDTAARQAEVRAMGDRIAELDRIVREAEEKLNNALLF
                     MPNLPHPAVPPGHDAAQNRVVRQHGTPRSFDFEPKPHWELGEKLGLFDLERGARMSGA
                     GFPLFTGRGARLERGLIQFMLDLHTTQHGYREIAPPFLCNAASMTGTGQLPKLAEDMY
                     HVASDDLYLIPTAEVPVTNFYREEIIRDPLPVKLTAYSACFRREAGAAGRDTRGLIRV
                     HQFDKVELVKFVEPETSDAELETLLADAEDVLQRLGLVYRILELCAGDLSFASSHTYD
                     IELWAPAQKAWLEVSSCSNFGDFQARRAGIRYRNKDGKAAFVHTLNGSGVALPRLVVA
                     LLENGQRADGSIVLPEALHPYLGGLTVLD"
     gene            7160..8530
                     /gene="tilS"
                     /locus_tag="PK634_00035"
     CDS             7160..8530
                     /gene="tilS"
                     /locus_tag="PK634_00035"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOW96152.1"
                     /translation="MLQIIQRAIRRQNLVTPGTRLLLAVSGGADSTALAAALAGLRVP
                     LNLRLTLAHLNHGIRGAEADDDARFVRALARRLRAGFVLGRADVPGLSRKKGISIEMA
                     AREARYDFLARAARRAGADAIATAHTADDQAETVLLRLARGTGPQGLAGIPYRSEHGG
                     VPVIRPMLDATRAQVEAFLKRRGLGWREDSTNRNPEHLRNRVRHEIIPLLEARLNPCL
                     REALCRLASLAGEENRWMEQEAARLAFDGFVAQLAGLPVPLARRALRAWLSKTGVPPE
                     SLDFEAIERVRALAEGRRGGGSAPLPGGWRVTREAGALRIQKAGRAGPSSYSTRLKVP
                     GVSSLASGWRVRVVRGRGYAAPSAAGNLEAWVDAGRVGRSPLMARSWRPGDRIRLVGL
                     AGSKKLQDLFVDLKIPREQRLRIPVIECKGEILWVPGSRVSRDWAVSHPQAPSWRLSA
                     RLPPAG"
     gene            8654..8830
                     /locus_tag="PK634_00040"
     CDS             8654..8830
                     /locus_tag="PK634_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96153.1"
                     /translation="MLLGFEDPWVCTVYILCIASTLLCVVYGLVNWNKGEETVQPEDV
                     QWAKEEKEEVEETL"
     gene            8871..10775
                     /locus_tag="PK634_00045"
     CDS             8871..10775
                     /locus_tag="PK634_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOW96154.1"
                     /translation="MDLFSIIIVLVYLLATGYLGYQGYRQTKTAADYLVAGRSAHPVV
                     MALSYGATFISTSAIVGFGGVAASFGMGLLWLTFLNIFVGIFIAFVVFGNPTRRMGHH
                     LDAHTFPELMGKRYNSKFLHVFSALIIFFFMPLYATAVIIGGAEFIAPIFRVSYDTGL
                     YLFALIVAAYVIAGGLKGVMLTDALQGAIMLVGMVILFITTYSMLGGIVPAHEALSAM
                     KDLVPKGLQAIGHRGWTAMPEFGWSAAGAPMPDALRHNLWWVMVTTIVMGVGIGVLAQ
                     PQLIVRFMTVKSKQALNRAVIMGGIFIFFMTGVAFIVGALSNVYFAQREKIECRIVAE
                     NVLMDPGADGKAKLTLVPENAADEVKARAKPFVSYRLSGDEERQPLSYVMQTPGTVIT
                     RGVDGAPDILEPHLVAVARSVTLGTPLGGNSDTIIPTYVKAALPHWFAVVFLLTLLSA
                     AMSTLSSQFHTIGTAVGRDVFEEVTHKRHTEKSILVTRFGICIGLLAAVIAGKVIRGN
                     IIALATAIFMGLCAASFLPAFVGGLFWRRMNKAAAVSSMVVGFVSSTFWLAFVNGKTA
                     AGLGLCKFIFGKDHIVPPTWSVTWTVVDPLIVALPLSILAAVVVALITKPMDKTYVDW
                     CYGGPPPKAE"
     gene            10851..12293
                     /locus_tag="PK634_00050"
     CDS             10851..12293
                     /locus_tag="PK634_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOW96155.1"
                     /translation="MKTSVRMLQCALLALAVLWTAEPAWAAKKKAKKADEPAKTEAVA
                     PAEQAAPPAFRYGADLRLREEYFDNIPIIADPPGVTRGGENNYFRVRPRIWAEYDAME
                     NVTLRARLVNEFRIYDKPDMDAAPQRSNYEFPDEYVFDHLYLDVRNLLGDKLDLRVGR
                     QELIYGTGKVILEGTPKDGSRTIYFNAVKAVWKAMPKTTIDVFGIYNPSIDDLAINDA
                     DRDLTGVTSANDDITESGAGLYLKNKSVEKCPYELYYVYKNESDWDKVAGTNAPVAIP
                     ESDIHTVGFRVMPQLAKDLKGNLEFAFQAGEQGDKDLEGWMLDTALSYTIPMAADTLA
                     PVADVGFYALSGDDPDTADKNESWNPLWARYPQYSELYIYSWDADAAGKWQNVMMPYV
                     QMSLKPCKKSKLTAMAGYLLAPEDNGPGSGDERGMLLVLKDEITIAEKLLSSKDKLTG
                     HLWAEILEPGNYYNVDDTAYFLRWELMYAF"
     gene            12495..13796
                     /locus_tag="PK634_00055"
     CDS             12495..13796
                     /locus_tag="PK634_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOW96156.1"
                     /translation="MIFNKTFECMDRAALRNLQGERLRDAVKRAYEHVPFYRSRLDAH
                     GVKPSDIHGIEDIVKLPFTTKEDFRDTYPFGLFAVPLREIVRVHASSGTTGKPTVVGY
                     TKEDIDTWAETIARTLGCGGGTADDILQVAYGYGLFTGGLGLHYGGEKLGAMVIPMSG
                     GNTKKQIMLMQDFGTTLLACTPSYSLQIAEEAAADDIDLKALKLRVGYFGAEPWTEAM
                     RAKIEERLPVKAIDIYGLSEVMGPGVASECLEQDGLHVFEDHYFPEIINPTTGEPVAA
                     GQKGELVYTCLTKRSTPLLRYRTRDISSLDEKPCPCGRTSRRMARITGRTDDMLIIRG
                     VNVFPSQIETVLMKVEGIEPHYVIVVDKTGALDELEIKVEVSEQLFSDEIRKLEELRA
                     RIFEEMRSVLSLSAKITLVEPKTIERTLGKAKRVIDKREVK"
     gene            13830..14261
                     /locus_tag="PK634_00060"
     CDS             13830..14261
                     /locus_tag="PK634_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOW96157.1"
                     /translation="MKVRQLSIFMENRAGRLAEIMQSLGDAGINIRALSLADTSDFGI
                     LRLIVSQVDKALEVLRKAGHTVSLTDVVAVEVPDHPGGLASVLTPLREAGVNVEYMYA
                     FVEKATDKAVVIFRFEETEAALKVLAGARISVLPAETVYKL"
     gene            14372..15646
                     /locus_tag="PK634_00065"
     CDS             14372..15646
                     /locus_tag="PK634_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOW96158.1"
                     /translation="MHVDALRELQLERLRKVVRRAWGHVPYYRQKMQVVGVTPSDIKT
                     LEDIRKLPFTTKDDLRLCYPYEAFAVPLEKVVRIHASSGTTGKSTVVGYTRKDMEVWT
                     NVVARFLVAGGLTSKDIVHIAFSYGLFTGGFGLHYGAERVGASVIPVSAGRSERQIQI
                     MRDFGSTALVCTPSYAVYLGELVQGMGLKPGDLKLRVGFFGAEAWSNRVREEIQAKLG
                     LFATDNYGLSEIIGPGVAGECVHQNGMHISEDHFYPERIDPDTCEPVGPDRRGELVLT
                     TLTKEAIPLIRYRTRDICMLMPGTCPCGRTGARMSKITGRSDDMLIIRGANIYPSQVE
                     EVLLDVKGTLPHYQLVVSREGALDRLEVRVEVSEEFFADEMKKLNELDRQIEHKLENV
                     LGIRASVKLVEPRTIERSEGKAKRVLDLRPKE"
     gene            15804..17852
                     /locus_tag="PK634_00070"
     CDS             15804..17852
                     /locus_tag="PK634_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96159.1"
                     /translation="MTIRSIILGLLGVAFVCSYTYFNDHVMRQTFFVGNNMPISVYGL
                     LVVFLLFIYPLFRKISRRLALSRREIVVILVMTLASCAIPGSNLLRLFTQTLILPHRF
                     ERTEPGWEKQKVMDLTPPGMLVDVSGDEERVLNGFVRGLSIANESIPVRAVPWKAWAR
                     PVAFWLPIILSLWLAMVALSVVVHRQWVAHEHLPYPIVTFTESLLPEEDGRRSPIFGN
                     KLFWLGALLPFAIHTYNYLVVWFPDQTLGKIPVGVDFRSLVELFPTFKKGGGEALLGY
                     WQLYFSVMAIAYFLPKDVSLSLGIGPFFWTLVAGVLTSYGIASTGWQGSWPFGIQREG
                     MVVMGSYFAMLLVILYTGRQYYRTVFARALGLRTKDPVEPALAWGARVFLAAVALFVA
                     YTAIVARLDWPLALLFGLGIIGVYLVMSRMIAETGLFFIVTYGTPATILWALLGARAL
                     GPQMLLIMFMLSVILFYDTREALMPYIVNSLKLSEDCKVRIGRAAGLMVLAVVVGLAV
                     GIPVTLYFQYNRGLDVAAWQNQQPKVPYNEVIGVMQRLEAQGQLEEAGTLTGLKRFAW
                     AGPNKLALTTFGAGAALVLVFSALRLRFAGWPLHPVMFLVWSTYSGQCFAQSFIVGWL
                     IKSSVMKYGGASIYQKLKPVMFGVIAGEMLGGMVPMVVSLVYYLVTGDQPKQFLIMPG
                     "
     gene            17947..19419
                     /locus_tag="PK634_00075"
     CDS             17947..19419
                     /locus_tag="PK634_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOW96160.1"
                     /translation="MKHIDVQPQPVLSFGRTFAIALRGVQYRLFRALVTVAVIAVAMA
                     FLMNILSESLFKRVVAVAVRDEIRVSRQADHWIARLSVPQTAEEVIDDLAAAEPGTPA
                     SRELARFSGRDPAAIEQAGKQAAQAREYFRFFDDINYGRRRVLIGNAAGIGIFERLQD
                     PEAQARFFESLGQMRAVRFIATPDGFRAFLASWPALREVVAAVREGQRQGIGQLHAKL
                     DGRTVMAALADAEGAFGEAVRQAGFVLEPEEATSLAKQIRLSEHRHFIEDSLNVPEVR
                     KAVAARKDVLPGDVSLDMIWGLLGHPDTAAWYRDLLAQHTSPPPGLDGDRLHEVARAR
                     GRSKLMVRAELITMGTGGGFMNIGPRMTWLAFVSMLVCTVGIANAMLMSVTERFREIA
                     TLKCLGALDGFIMTVFLIEAAILGLVGGVAGTLIGLVLGLGRMLAVFQSLLLELFPGG
                     LLLQAAGISMVTGIALAAVAAAYPSLRAARLAPMEAMRIE"
     gene            19508..20263
                     /locus_tag="PK634_00080"
     CDS             19508..20263
                     /locus_tag="PK634_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96161.1"
                     /translation="MAKKEQAPDSAEKRVIIRTIGMKKLYRKGGQETAALRGVDVEIY
                     TGEFISVMGPSGSGKSTFFNMIGALDSPSEGRVFIDEVDVAQLTAEELAFLRCRKIGY
                     IFQSYNLIKYMTALENVTTPMAFGGVPDDDARKRGMEILGLVGLRERWFHRPIEMSGG
                     QQQRVAIARALANQPAILLCDEPTANLDHHTGQEILDILQKLNKECGVTIICATHDHR
                     MMNVCDRLMWIRDGSIERIARRDEVHVELAGID"
     gene            20266..25137
                     /locus_tag="PK634_00085"
     CDS             20266..25137
                     /locus_tag="PK634_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96162.1"
                     /translation="MRAGASFYSVAVLERAGKRSATALGWLAALLLATAARAISPEFL
                     ADCQALTRHPHRLAGSEEYADAARHVEQRLREAGVTNVLVQTFPTVQNVVRRCEAVAA
                     DGRTFPLLPMRPDGIVPPVTPPAGITGRILRLGRGEPGDFDREDPRGRIVVMNYNAED
                     RWLKAFRLGAKAVIFVKEETADAFHFHYVRANANLPRFYYDGPASDLADGQELTIHSE
                     VAWQRATGRNVFGFIEGKVPLFDLGKEEVLLIAAPLDTFGEVPQRTPGAQPAANAAAV
                     LKLAGMLAKAPPRRHVLLAFFDGHGRNHAGVAAFYQALETKARDATIEERMAAWKGES
                     EFLAETEKLAAQPNPLAGHAGDAGRELLLRMRNKAADRAYEIRGRLMDLREARSKAGR
                     DSPAYADLQAREDELNGVYNRWNELRRKLGKGGADAAGSEEFDAALAGVRRDIALRRA
                     ELVEEEQMLRSSAAIKALLGERWIALHATMMLGDRTPTWGVVVGGNSEIRSTQDLPGL
                     YGRVQRSFLQAHRSLARRGNGAPHFEPVSVDGSLRDVRLLWAAPMLIHGGELAGRHGV
                     YNVVLGTLHETLPRLGTPDDTLDRLDADRIEQNTAEVALLLAAVGSLEGASGDVEAVA
                     DQRGLSLRRAIMADNDYVVSEFSGGRTQGALVMGRHRGSSIPNRPVPGAVVQVSVVDP
                     WNSVFWRHSKAPGFDPFTVVMTDQNGAYSLNALPTQDPAPIAFAAAFDGRGLVNQVTD
                     MTSRKTVRQRLNMFRCAGGLLVLPPMHWTQKATFMSARANAPLAQPTATDSYGETTDG
                     VAYWYGEEKLPGIKAFGKDALVALNNVPVEGQKPDPYGRGYPRSAEWTTLRNTEGSAW
                     DLWRLNEARLSILRSRDILNSALEEAHGRVRDLLDEAAAGPSLVRNEALCASAFEAAR
                     PVYTQVRESMDDLVKAVLVLLALCIPFAFALERLLIGASLIYKQVAWFVGFFVATFLL
                     LYVSHPAFAIAQTPIIIFLGFAVVVLSVLVIVIIMQKFQVELKRLQGMTSTVHAADIS
                     RFSTVMAAMAMGISTMRRRPLRTTLTAVTIILLTFTILGFASFDTQRGIVKLFVAPAP
                     PYTGVMLNNPTWGSFNAEFVDLVRSRWSREVNLCLRYWICPEFKDDPDFVIALEDGSK
                     PVSLKAVLGIPAEEIAARPDLKQLFRLNDPADIDRMVLMTEAVATLLKVKPGDRVCLK
                     GKILEVGPLLSAADLSAAREMEGSSILPVDFATMKLASAGTAEVTENVLDEQAKWTSL
                     PVDSVVITSSENSRLFGGKPHLVHFYTKDAQEAAGIAEELARMLDRHPVMATRMDGVY
                     RHVQGTVVAASGVGDLFFPILLGGLVIFGTMLGSVADREKEIYTFSALGLAPPHVASL
                     FFAEAMVYSVIGGLGGYLIAQGTLKVLTVAANYGLVRVPEMNYSSTNAIVTILIVMAT
                     VLVSAIYPAIKASKSANPGLMRLWRLPPPEGDIFNIVFPFTVSAYDLTGVVSFLKEHF
                     DNFGDVGLGSFMARGTAVRSLGGGELGLTSQLALAPFDLGVTQLFELRSAPSEIKGID
                     EVNIRLTRRSGQPKDWERLNRVLLDDLRTQFLLWRSLPQETMEEYRRRTLVALNREGG
                     GPPPPVSQAKETI"
     gene            25140..27113
                     /locus_tag="PK634_00090"
     CDS             25140..27113
                     /locus_tag="PK634_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOW96163.1"
                     /translation="MAQRIDKELEEFRQVMEVPSSFEEGFNWTSLLGAIFVALLMVPG
                     AIYMGLLAGVENIGSASQWVTVILFIEVAKRAQKTLKRAEIFVLFFMAAAALGMPFSG
                     LIWNQFFACSDAAIAAGIAEHLPRWFAPPAGSESYALRTFMHKDWLPVIGMVIFGMFF
                     GQLSNMVLGYGLFRLASDIERLPFPMAPIGAQGVMALAEDSDEKGAEEGEGRWRWRVF
                     SIGGALGLVFGTLYLLLPTLTGALTGNPIQLFPIPFSDFTPQTGEYLPAVATGLSWDF
                     GNIIFGMVLPFFAMVGSFVGLIATVVLNPLLHAVGVLRNWVPGDSTIGTLYKNNIDFF
                     FSFNIGLALAIALAGFYQVIRSFQGRRKTAGGEPVRLAPAGRGDIRPFIVVVVYFLVT
                     ITYILVSGWMVDWHRGIMIVLLFLGFVYTPVISYVTARLEGMVGQVVEVPYIREAALI
                     LSGYRGVAVWFLPIPMANYGQMAIFYKQCELTGTRFPSIWKTQVILFPIILLSSIFFM
                     NFIWSLNEVPSAVYPYAEMMWKLNAENACIMFSSTLGEYSIFEEAFNVLYIAIGAVFG
                     VALFGAMNTLGAPIMLTYGVVRGLGGVMPHWVIPQFIGALIGRYYFQKRLGLRWRQYI
                     PVVSAGFACGMGLITTVGVGITFLSKASIPLPF"
     gene            27126..27848
                     /locus_tag="PK634_00095"
     CDS             27126..27848
                     /locus_tag="PK634_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96164.1"
                     /translation="MAEVLIEIRGVNKVFDLGKIKVHALRDMDLEIFRGEYLSIMGPS
                     GSGKSTLFNMIGALDRPTSGTVTIAGVDLATLSSRELAFFRGKHIGYVFQTFNLLPAY
                     SAVDNVAMPLVFNGAGEERAQARAKEILERVGLGHRLTHRPDELSGGQQQRVAIARAL
                     ANEPAIVLADEPTGNLDLHTGEEIIQLLSELSRSMGVTVISATHDHKMLATSDRILWI
                     KDGRQDRLERREDLNIRVGTVK"
     gene            27863..28147
                     /locus_tag="PK634_00100"
     CDS             27863..28147
                     /locus_tag="PK634_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96165.1"
                     /translation="MKMWKLLTGHPWIWTSLFILFAVVHVSLALAIVSDGKRLGARGS
                     GTFLVGPWIWGMATLMGGVYAAGIYWIIHYSRLKPASRGMAQHGQCGPVD"
     gene            28211..29200
                     /locus_tag="PK634_00105"
     CDS             28211..29200
                     /locus_tag="PK634_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOW96166.1"
                     /translation="MSDLRKLFWAAAVLLCGALGPAASEEIRSSVVVVVRNDQVWDSE
                     GNLDPDEIGRMMEMGLQALAGASSGRAAFERLFKPGERIAVKVNAITKNWMSSSKPAL
                     VQQTVDLLQQVGISPDHITIFDRMPRPGRHELGPAGFALNTNAGSVQVVEGGMLGPER
                     QQGSLKTRFYAPVEECDALISIPVLKDHHIMGMTFALKNHFGSIHNPWSCHQNAGVPG
                     IAELNGLEPIQKKQRLIIGDALAGQYNKGPHDAPKYHWKENALIFGFDPVAVDFVAFE
                     ILEAKRRQEGLAWPESMRYGGKAGAYILDCSQRGVGRKPDRVIQLRCSSDTGP"
     gene            complement(29245..30120)
                     /locus_tag="PK634_00110"
     CDS             complement(29245..30120)
                     /locus_tag="PK634_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOW96167.1"
                     /translation="MKHARPILALLLAAILPAAADPAPNPEYVDGYQTINRFAKRLGK
                     LVQAKSAVPMKALIEQAKTFKPCALEALPSSGAVQSPEEIYASCRDSVVLVGAVYKCK
                     KCARWHLASATGFAVSDDGLIVTSRHVVSSNSFCEAMGILTRDGRLFPVKEVAASDPR
                     NDLVILRTDAQGLRPLPVAEDISVGAPVYAIHHANPNLYAFTQGAVVGKFLSGRLNPK
                     EPLRTLAISADYAPGSSGGPIFDQHGAVAGIVCSVSPVMDHGHDSTNVWASMIWKYSV
                     PSDALLELMEAPVPP"
     gene            complement(30133..30348)
                     /locus_tag="PK634_00115"
     CDS             complement(30133..30348)
                     /locus_tag="PK634_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96168.1"
                     /translation="MTEPETATPAQPAITWSEQHLPTLLRFWKAFGRVPPLPDRAAQF
                     LTALLGILGLLAFVTLIAYAVEITSRP"
     gene            30469..32586
                     /gene="buk"
                     /locus_tag="PK634_00120"
     CDS             30469..32586
                     /gene="buk"
                     /locus_tag="PK634_00120"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOW96169.1"
                     /translation="MKKRAVSPPPVSVGRLQEVASGLPPKTVVIVGGDRKEDLHVARS
                     LRGLPFVRRCVLVGDGAAIRDAARLVDLAVPDEDILATGSPEETARRTVDLVEEGVGE
                     ILLKGNISTPVLNREMRRIRSRDTMSLVTMFQAPSLAGGRPVLLTDAGVTTLCTFSRM
                     TGIIRNAIEVAQYVLGIARPRVALLSANEKVIESLASTKMAAALAGKYWEDAVVCGPL
                     SFDLATDPESVRIKGLAASEDPAFREVAGRADILVCPGLDAANILYKTVMAMTQHGLA
                     ATAGITAGVKVPYIILSRADGEATKLDSVALCCIYAERRKQKQLEQERRVAVARQEER
                     VFHVLAVNPGSTSVKVALFRNAECLDSNELSHPHRSRLAGAAFDEEVGRYLELVKAFL
                     KAHAELRLDAVVGRGGFLHRGGRQLEGGVYRVATVQDGRVRIEEDIVRGVRDCPEMDH
                     ASNLGIPMAARLAVEYGVPAFTVDPVVSDEFDPLAQLSGYAPITRRSTAHVLSVRALA
                     GRAAEAIGRPLEEISLVVAHMGGGITIAAVRNGRIVDNNIALLGGGPFTPQRAGALPT
                     KEFAELCYNGKFSKEELFVELTKRGGLVSYLGTSDVEKIQDRITQGDTRARDVLEAMA
                     YQIAKEIGAMAVAAGHDAEAIVLSGGLARSELIVGFIRKRVGHLAPVMVYPDNLEMAA
                     MAAGALRVLTGRVEARTYVLAAP"
     gene            32754..33698
                     /locus_tag="PK634_00125"
     CDS             32754..33698
                     /locus_tag="PK634_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW96170.1"
                     /translation="MIKVEHVVKKFGARAAVDDVSFVVEPGTVLGFLGPNGAGKTTTM
                     RIIVGFLSPTGGRVEVCGREVAAEPSRTQASIGYMPENTPLYDEMTVEEFLRFIAELR
                     GFRGAERNRKAGEAMESCALAPVRRQAIETLSKGYRQRTCFAQALLHDPPVLLLDEPT
                     NGLDPNQKQVVREMILRMAPRKTIVLSTHVLEEVEAVCSRVVIISRGKVVADSTPAAL
                     KQRSRSFNTLTLELVAPADQAEAGFRGLPDVERVAVLERRDGGACRLQLAPRNQQPLA
                     SAALEFARGRGWLVTDMQNDGGRLDEVFQQITRTDDAA"
     gene            33710..34456
                     /locus_tag="PK634_00130"
     CDS             33710..34456
                     /locus_tag="PK634_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96171.1"
                     /translation="MESLRHIRSIARREWSAYFNSPIAYVFIVIFLALAGFFTFSVAR
                     FYEEGQADLRGFFFWHPWLYLILVPAAAMRLWAEERRSGSMELLLTLSVTPAQALLGK
                     FLAAGLFLLLALGLTFPLVLTALYLGSPDLGPVISGYVGSALLAGAYLAVGLFTSALT
                     KNQVVSFVLAVVLGLFLILAGFPPVTDLLAQWAPEWLVNGVAAFSFMPHFDTLQRGVL
                     DLRDFAYFASVIGFMLLATTLVVRGQTSRA"
     gene            34477..36327
                     /locus_tag="PK634_00135"
     CDS             34477..36327
                     /locus_tag="PK634_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOW96172.1"
                     /translation="MSKKSLRIGLSGAAGALIVLAILVAVNVILGAARVRKDLTAERI
                     YTLSPGTRAFLGGLERGVTLKLFFSRGNENLPVGFKQYARRVTDLLREYEAHGGGRVA
                     LETYDPQPDSDEEEWAQRYGLAGQNLDRYGMEPDFYFGLVAVSGTREAALPFLSPGAE
                     PQLEYLVTRLIHEVTRAGKPKIGVLSALRVMGPPQRPLGMTQDEAGGWQIMTELARQC
                     ELVPLTDELETVPADIETVLAIHPKGLPDKTLFALDQFVLRGGRLLAFVDPLCVADRE
                     SGLAADPYGTGPASSDLNRLTKAWGYELVPGQVVADVEAASYVTFGGRGSERLPTWLS
                     LRPDNINRDEIATAALENLMMPFAGAFKGAAAEGLTAVTLAQTYDEAALLPAFQAAQA
                     GVEKMRAAKPEGSLVLALRLQGRFKTAFPDGAPAAFLKEGEKDSVVILVADADMLHDR
                     NSLQEMNFLGQRLLQPVNDNLSFGLNMVEQLTGDEALIGLRSRGTYGRPFDRVVELEK
                     QAQQRWQEEELKLVEKLREAQARLNELQRSKDEDQQYILTPEQKREIENFRQERFETQ
                     RQLKEVRKSLRRDIERLGLKVKILNMAAMPAAVALFGLLHGWRRKKKASS"
     gene            36351..37547
                     /locus_tag="PK634_00140"
     CDS             36351..37547
                     /locus_tag="PK634_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOW96173.1"
                     /translation="MSPKTLALLLVILVALVWWQGHPRAGGTERAPGSAILPGLDVNA
                     IDRIEVASAAATSVVERRDGQWVVPSLFGHPADFQKVADALRGLADLKVGQVVRDAGQ
                     YPEEFGLAATSPVFRVALSGASGFHAALELGATKREGAQEGFAGYESGRYLRAAGGTV
                     VLVADPLQSFADASGEWIRKELLNVAPPELDRVTVAVFNAEHVLLIKGSNQYEVEGLK
                     DGEETDPAGAARLVGALQSLSCLTVADPALTDEAAGFGAPDRCTVLARNRMEYTLQLG
                     GAGPEAGSRYLRIDVAYREPAPPTLEQVAKSIPVAAESAGSDTGRVEKVQAEYRERLQ
                     AHEAEIAARREERGRLEKRFRGWTYVIPAGTAESLILPRGQLVKAKPPVAPAAVSPQG
                     QDQGPP"
     gene            complement(37551..39005)
                     /gene="mmsA"
                     /locus_tag="PK634_00145"
     CDS             complement(37551..39005)
                     /gene="mmsA"
                     /locus_tag="PK634_00145"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOW96174.1"
                     /translation="MRYSNLSNFIDGKSSNPGVPRMERYSPVDGELLSSLPLSDAKEL
                     DKAVQAAKKAFADWSQWTIKERTGVFYKYYELLSKRLPEIADIQYHEMGKIPAEALQE
                     VGKSLELTEVACSMPQMATGDFEEVSRGVECHDVRVPLGVVASITPFNFPHMVPHWTL
                     PNALVLGNTMILKPSREAPYTVLKIGEMMKEAGLPDGVLNIVCGGREVVNAIVEHPNI
                     QGITFVGSTKVAQMVYAKGCANFKRVLALGSANNHLILLPDAHPANSAANIAASYLGC
                     AGQRCMSACVLVAVGDVDPIIAKLVEESKKFVPGVNMSAIVNTEAKANIIGYIDRAEK
                     DGANILLDGRGAKPPAGKEKGQYLGPTIIEAKAGSEIAMEENFGPILTIVRARTLDEA
                     LDIANSSPYGNGGSIFTQSGRAAEIFCKKIQAGMVAVNVGVPVPREPFSFGGWKNSIV
                     GGGDITGKSSFGFWTKLKKISVKWNPDKPVNWMA"
     gene            39487..40362
                     /locus_tag="PK634_00150"
     CDS             39487..40362
                     /locus_tag="PK634_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOW96175.1"
                     /translation="MARIVKAGLIQCSLPLHEGEGSMEEIKDAMLKKHLPLIDEAGRK
                     GVQILGLQEIFTTPYFCPSQDPKWFRTAEKIPDGPTVALMQKYAAKHQMVIVVPIYEE
                     AMTGVYYNTAAVIDADGTYLGKYRKNHIPQVGGFWEKYYFKPGNLGYPTFQTRYANVG
                     VYICYDRHFPEGARLLALNGAEIVFNPSATCAGISQYIWKLEQPAHAVANGIFMGCNN
                     RVGVERPWNIGRFYGTSYFVDPRGEMLAVGSEDKDELVVADLDLDQIREIRDKWQFFR
                     DRRPETYGDMTKLLP"
     gene            40413..41798
                     /gene="hydA"
                     /locus_tag="PK634_00155"
     CDS             40413..41798
                     /gene="hydA"
                     /locus_tag="PK634_00155"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOW96176.1"
                     /translation="MSIVIKGGTIVTAGDLCAADLRIDGEQIVEIGRDLPAKAGDTVV
                     SAKGRYLFPGGVDVHTHFELPFMGTVSADDFKTGPVAALCGGTTTYIDFCMQAKGKPL
                     AASLAAWHEKAKKSAADFGFHMGIVDYTPAVAAEIPAVIKQGVSSFKFFMAYKGALQI
                     DDGQIIGIMETVGRNGGLSMVHAENGDMIDTLSRRFIAEGKQTPEYHWLAHPAIAEEE
                     AVHRIVELARFTEQTIYIVHLSSADGLEKVKEAVARGYPVIAETCPQYLLLSSDLYYR
                     PNFEGAKWVMSPPLRPADHLEKMWEGLGRGFIKTVATDHCSFNFKGQKEMGRDSFAKI
                     PNGIPSVGDRFNLLYTYGVGQGRMSLTRFADVVATAPAKIFGMYPRKGTLAVGSDADV
                     VIFNPEAKGVVSAKTTKHNVDYSAYEGLELKGLPESVLLRGKFAVRDGQYVGEQGAGR
                     FIARGPAGKMV"
     gene            41858..43195
                     /locus_tag="PK634_00160"
     CDS             41858..43195
                     /locus_tag="PK634_00160"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOW96177.1"
                     /translation="MNKDKIAEIKQWDAEHVIYPWKTQGWYRPILMERAEGIHMHDAE
                     GKRYIDFCSGLLNINIGHGNKHVLDAMKAQMDKLCYVASVFATEPKAKLARMISEVTP
                     GDLDYVFFTNAGAEAVENAIKAVRWFTGRQKIYSNWRGYHGATSGAITLTGDPRRIPC
                     EPGIPGAGKFFGPYCYRCPFGYKDEKSCGIQCLETLKTQIMLEGPKTIAGIFMEPIVG
                     TNGIIIPPVEFMKGVRKLCDEHGILLVMDEVMAGWGRSGTWFGFEHFGIVPDIITAAK
                     GLTSGYVQLGVMIWSKKLWDFFKDKPFISGLTYYGHALACAAGVANLEVYKSEGLIEK
                     SRINGDYLNNKLWSLYERHPSIGDVRCKGLWACLELVADRKTREPLAGYADAKRNVMG
                     EFTKRLLEMGLWAFAKWDYIFLAPPLIITPEQIDEAVAILDKALEYPDSLLKG"
     gene            43218..44480
                     /locus_tag="PK634_00165"
     CDS             43218..44480
                     /locus_tag="PK634_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOW96178.1"
                     /translation="MDLHQIISFFGTVILLFIGWLFCKHRKSVHWRTILWGTALQLIF
                     ALLVLKTPPGRWLFNFMNDAIVKLLSFQEEGGKFVFNALSIPVGQPGSMGFFFAFQVL
                     TTIVFFSSLISVLYYLGIMQKVVQFFARIMFVTCQTSGAETLSASANIFVGQTEAPLL
                     VRPYIEDMTESELMCVMTGGMATVAGGVMAAYVGMLKGYFPDIAGHLLAASVMSAPAA
                     LVMAKIMIPETGHPKTRGTVAIAYKDPSVNVIEAAANGATMGMHLAFNVGAMLVAFMS
                     LLAMANWILQHAIAWFGPEGVTLQRLFGWAFAPLAWVMGAPWKDCQVLGQLMGEKTVL
                     NEFVAYTSLMQHAAAHPGGLAYRSYVIATYALCGFSNFLSIGIQIGGIGAIAPSRKRD
                     LARLGIYALIGGSLASFMTATIAGILVP"
     gene            complement(44541..46280)
                     /locus_tag="PK634_00170"
     CDS             complement(44541..46280)
                     /locus_tag="PK634_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOW96179.1"
                     /translation="MNVLWLVGVALPVFFLGYRFYSRYLQRALTGGTEEPPPSVTMKD
                     GVDYVPSRTPVVFSHHFASIAGAGPIVGPTVALIYGYIPVWLWVVLGSVFIGAVQDYA
                     SLFVSMREQGHSMAQVANRTMGRFGFFLVVSFSIIMLLLLTSAFLGLSATALTSLVPL
                     EQLRIEAGRTFLRVVAGADGLARAKVGGVASTSLIIITLGAPLIGFLVYRRGMTMRSM
                     FPLAMAICLASIAAGLKYPLTLSPMAWMLILTFYVILASGLPVWVILQPRDFINSFLL
                     YAGMAILIIGSAAGGAGGADPNMPAWNLAGGRQSLGMVWPFLFITVACGAISGFHAMV
                     TGGTTAKQLSSERAARGVGYGGMLLEAVLAVLVIVAVATGLSLEQYQGLVYPAAKGVG
                     SNPVLAFALGMGSLVHRGLGLRMEYGTVFGMLMVEGFIVTTLDTAARLNRYLLEELWA
                     VLFKRAPSLLRSHLFNAVVVSGLMFVLCYTNTFKHIWPIFGSANQLMAALTLIVVSVW
                     LAARSRPSWFAVAPAVFMMATTLFSLWQLLTGQYLPNRNYPLTVTAIVLMLLAVGVMI
                     LTLRRWSLRRQPS"
     gene            complement(46305..47576)
                     /gene="preA"
                     /locus_tag="PK634_00175"
     CDS             complement(46305..47576)
                     /gene="preA"
                     /locus_tag="PK634_00175"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HOW96180.1"
                     /translation="MSKQADLSVEFLGFKMKNPFMLSSSPVSNSAEMVGRAFDAGWGG
                     VAYKTVVSDRIPIIHPSPRMAAYRPAGKRQLGVQNVEQTSDRGLKANLLDIMYLKKRW
                     PDHLVMASIMGFSNQEWADLAKACVDAGADMLEVNVSCPHMTVEGSGAKVGQCQELVQ
                     KFTETVRNSCKIPIVAKMTPNITDITVPAMYAKKGGADGIAAINSVAGLSGIGADDWV
                     PSPNVFGKGAACGFTGPAIKPIGLRCISELAKCHDLQLPLSGIGGIETWVDALEYLLV
                     GATTIQVTTGIIHYGQGIVENMIEGLSDYMTIKGVKSLKELIGKALPNVHATHDFDLA
                     RQGIARYDLDRCVGCGQCYVVCRDAGGWALDWDAKKRRPALDEKKCLSCMVCSFACPV
                     DGLITFKEMPKSYRRAETVTLGKELETQRRS"
     gene            complement(47569..48918)
                     /locus_tag="PK634_00180"
     CDS             complement(47569..48918)
                     /locus_tag="PK634_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW96181.1"
                     /translation="MPKAPAPFASMKKGFDYGQAVAEAQRCLLCHDAPCSKGCPGGTD
                     PATFIRKLRFQNLTGAVRTIKQNNILGGSCGELCPTPQLCEKECSAAGLDRPIQIGKI
                     QRFLVEHAWATGFNPLEKGSARKEKIAVVGSGPAGLSCAAELAKAGFGVTVFEARARA
                     GGVLRYGVPSYRFSPDFIDRELKDLAPLGVKIQCSKPIRGKGAVESLLKKGFAAVFLA
                     PGCWEPLRLKVKAKVRQGVFAWTDFLTPLKEGRFAAIGKQLKGRRVAIIGGGSVAMDC
                     AESAIKLGAKDVYLVYRRSYNEMPAEEDEKQDALRQGMHLLLLNQPVDYSTDAKGRLK
                     AIRLVRTELSKPDASGRRKPVEIPGSNWELPIDVAIEAIGAQPARDSIEWSRSMKLSD
                     GNLITVNPDTGETSVKRVFAGGDIVRGPALIIEAVRDGKTAAQAIMKRFAKKENAHE"
     gene            49186..54312
                     /locus_tag="PK634_00185"
     CDS             49186..54312
                     /locus_tag="PK634_00185"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96182.1"
                     /translation="MKLFHVRLAFGLLGVGAFLVPAHAVTRYVAGNGGHVPPFTNWTT
                     AATSIQAAVNSATNGDWILVTNGVYGGNVLISNKTISLVSMYLYSGAISDAESTVILG
                     TGSGGAVRIVNGVGTSGRFMGFTITNGYANGSSTGEYDRGGGVYVAASGPRLEALRIR
                     GNRATSTGGGLYLEHATSVVNNVVVENNQSSGGSAGAGFYYGKPSLENLVVRDNVASS
                     SDGGGILFYHCNATCRNMLVVNNSTTEDGGGMHFDDSDVLLENTTVYGNRARTGGGLN
                     ISYSSSPRLVNSIVWSNTPDNIGFCTQWFGMDIRVDYSDVQGGSNGVVTYGKGPVYWG
                     AGNLDSDPMFDAGDFQLLDGSPCVNAGDNRDWMTGAADLAGAPRIAGPKVDLGAYENP
                     NGASVPTVSVPATPAGPASGLAGPSLAYVTGGSTSSLGGGVEYRFDWGDGNLSGWDAN
                     TEAAHAWSATGLFAVAAQARSAVSADVVSAWSGALPVWITNAPPVPDDHYVALTGSDE
                     YPYTNWAMAANRVQDAVDAATHGDTVWVTNGTYEGNVRFNGKNIRVTSLFTVSGDAAD
                     IETTVLQGIGSGSVVRITSGETVDSALIGFTVTGGYADGPADGEYDRGGGVCVRFSRP
                     RLEHLRIVGNRATSTGGGLYLEHATSVVNNVVVENNQSSGGSAGAGFYYGKPSLENLV
                     VRDNVASSSDGGGILFYHCNATCRNMLVVNNSTTEDGGGMHFDDSDVLLENTTVYGNR
                     ARTGGGLNISYSSSPRLVNSIVWSNTPDNIGFCTQWFGMDIRVDYSDVQGGSNGVVTY
                     GKGPVYWGAGNLDSDPMFDAGDFQLLDGSPCVNAGDNRDWMTGAADLAGAPRIAGPKV
                     DLGAYENPNGASVPTVSVPATPAGPASGLAGPSLAYVTGGSTSSLGGGVEYRFDWGDG
                     NLSGWDANTEAAHAWSATGLFAVAAQARSAVSADVVSAWSGALPVWITNAPPVPDDHY
                     VALTGSDEYPYTNWAMAAGSIQAAIGAAEDGDTVWVASAEYRENIDFTGKNIRIVSLF
                     ELTGDPADIAGTIIRGTGTGNVVRAVSGESTNALLKGFTIVNGHAVGVHPFNRGGGIL
                     CQYGSPRIEHVRVTGNHADIVGGGIYLEHSASALRDVEVAHNTAGGGGGMGCYYGQPS
                     LVNVCLQSNTATQSDGGGMILYHASPLMRNVLVADNTAPAKAGGIHFDAANPVMENLT
                     VVSNSAAHGGGLNVSYSSAPVLRNSIIWGNSPQQVEFDDWWFAMALTVEYSDLQGGLA
                     AIETHGLGPVHWLDGNISADPVFGDANGRLSGISPCLNTGLAQPWMAAETDLDGQPRV
                     CGASVDMGAFEYSSAAMPVGSLRCVMEPSAVLPLGARWRVPEGPHTNWNESGEVLHNL
                     ATGEYTIVFSEVAGWTKPADAHPVVVEGLETLETGLYLSMAPDTGPPVIVSVEPPSGF
                     VTTTNHLDMLITVTDNVAVARVTVNKHDATCVGGDQWAYTVHGVRGAYHVVEIVAYDT
                     AGNVASGEVVYAREHGITLHPVWAGYWRIRNPFSVSYSYRWEAVETAETGSGVLVPHG
                     DTYFTTSPQVEHVQLYVDEVLIETKEASEAEFPAPPSDERFIDSDGDGLLNMEEDAAD
                     TDPFSPDSHFMASLLSPDGAAPQPERLARQAVGSDFSLAWPSSADNLYTVEISLDLWD
                     WAAVPGYTDKPGTGSTMSYTNASAAALFFRLKTQPAEPEP"
     gene            complement(54322..55647)
                     /locus_tag="PK634_00190"
     CDS             complement(54322..55647)
                     /locus_tag="PK634_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOW96183.1"
                     /translation="MSLLIRHAKVLRILPGPQTRVLNEQDVRIEGARIASVEPASDNP
                     APADEVLDARGMLLMPGLINSHSHVPMVIFRGLAEDVSLEKWFNEYMWPLESNLQEED
                     VYWGMLLGLAEMIEAGVTCVADHYFFMDRAAEAVQQAGTRAALGWAVFGNQGIEALDR
                     TAQFCRRWNGAANGRITTWMAPHAPYTCDDDFLGAARDRADELGVGIHIHVAETRDQT
                     AAHVKKRGVTPIRLLDDLGLLRLPSLLAHAVGATEEDIRLLADRPAAVAHCPKTYLKL
                     AMGIAPVVAMRKAGVTVGLGTDGAVSNNTLDVWESLRLMAMTQKQATGAAENMTLAES
                     LEIATRGSARAVGLGDRIGTVEPGYLADLILVDLGGPHHQPLHSVPASLVYAARASDV
                     QTVIINGRVIMRDRRLLTLDKAEIIRQVSRSLERLSRRVPASRIQVYAP"
     gene            complement(55694..56344)
                     /locus_tag="PK634_00195"
     CDS             complement(55694..56344)
                     /locus_tag="PK634_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96184.1"
                     /translation="MNPDRQRMNRAIDGFVEAHYYSEAPEFESARRGFVDSLCDGLHD
                     LYRQTVLDRLRADPSLVNVLLCAGGDIPGAGPWLAARLDGETGTSPLSRALIRVLADY
                     PGEEACRAVERFLESDQQAEALQALARLDWPRVLPRLVAAFEKASLHPTILHILHARR
                     KAAGLPTLIEDLRRGAATWPGWDAEGWQRVFSGTPAPYNPFHPDELAVLIKAVRPA"
     gene            complement(56341..56529)
                     /locus_tag="PK634_00200"
     CDS             complement(56341..56529)
                     /locus_tag="PK634_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96185.1"
                     /translation="MTWEQAVVAMIVAGAAVALARAGWRSAARRRESAARCAACACPL
                     SRPGGRCALPDRGPECPP"
     gene            complement(56547..58613)
                     /gene="feoB"
                     /locus_tag="PK634_00205"
     CDS             complement(56547..58613)
                     /gene="feoB"
                     /locus_tag="PK634_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOW96186.1"
                     /translation="MSRPLVVALAGNPNSGKTSLFNALTGARQHVGNYPGVTVEKREG
                     RCRHGVREITFVDLPGAYSLTAYSPEELAARHFLIEERPDVVVDVVDASNLERNLYLA
                     VQLMELGAPLVLAFNMSDRARARGIEFDLARLSELLGVPIIQTVGHQGRGLRELLESI
                     AAVADHPARRPPRRFEYDAELNDAVDALERRLAERADLARRYNARWLALKLLENDAEA
                     QKLANEPAILEEVRRRAREIERIRGMPPEQVIADHRYGFISGACQEAVHATTEIRHTM
                     SDTIDSIVTHRALGLPIFLGLMYAVFLLTFTLGEPPMAWIERGVEALGARVNGFWPAG
                     SASPLRSLLVDGIIAGVGGVLVFLPNILLLFLAIAFLEDTGYMARAAFLMDRLMHKIG
                     LHGKSFIPMLIGFGCTVPGIMATRVLETRRDRLTTMLVLPLMSCGARLPIYMLIIPAF
                     FPERWRAPILWIIYLVGIGLAIGLARLLRATVFRGESMPFVMELPPYHMPTWRGMLRH
                     MWMRGGLYIRKAGTLILGISIVMWALTSYPKLDRFPDGLSGAARQAEGLAHTAAGRIG
                     QAIEPVLRPLGFDWRIGTAMIGAFAAKEVFVAQLGIVYSVGSDEENVEPLREALARHY
                     TPLTGFCIMLFMLIAMPCMATFAVVGRESGSWKWAVLQSGSLTILAYVITGLVYQGGR
                     LLGIGL"
     gene            complement(58610..58852)
                     /locus_tag="PK634_00210"
     CDS             complement(58610..58852)
                     /locus_tag="PK634_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOW96187.1"
                     /translation="MSAGHTLKDLKPGERGRIVRLDRRGALGQRMADMGLIPGSVVEV
                     KRIAPLGDPLEVQVRGYRLSLRKAEAAGVQVERMPP"
     gene            complement(58849..59307)
                     /locus_tag="PK634_00215"
     CDS             complement(58849..59307)
                     /locus_tag="PK634_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOW96188.1"
                     /translation="MSSSLEDYLEAIHLLTAHGASARGRDIAERLQVRRPSVTLALRA
                     LSARGLVNYAPYDRVTLTAKGRTAARDISRRHEALRRFFVKVLEVEEREAEQVACKME
                     HALSPAIRRRLTEFARLMKRCPLGGPRWINGGGFHCPAPAARRPRRTSRK"
     gene            59444..60181
                     /locus_tag="PK634_00220"
     CDS             59444..60181
                     /locus_tag="PK634_00220"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOW96189.1"
                     /translation="MADSRDTLSLDNIRNVLVRLEETILFGVLERAQFRQNAVIYRPD
                     GVGRGLGGESLVQFLLHECERSHAKVRRYTSPDEHPFFHDLPAPILPAAAYDNPLRPN
                     TLNINDRIWAAYVGEIIPFLCAPGDDGQWGSSSVNDVHLLQALSKRIHYGKFVAESKR
                     RARPGAFDPLIRAGDSDGLRAVITNADVERGVLERVEHKASTYSREIEAIPGQLAASA
                     ASVRELYARWIIPMNKDVQVLYLLQPG"
     gene            complement(60185..60260)
                     /locus_tag="PK634_00225"
     tRNA            complement(60185..60260)
                     /locus_tag="PK634_00225"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60225..60227),aa:His,seq:gtg)
     gene            60460..61899
                     /locus_tag="PK634_00230"
     CDS             60460..61899
                     /locus_tag="PK634_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96190.1"
                     /translation="MRVLFLYPVPPARWQRLRYQQGIGSICAVLKQAGHEVELLVLSE
                     PDRDRIAEAVRRFNPGLAAISLTSAEFDVACAMGGILGREHRLPVILGGIHPTLRPEE
                     SIAADGVFAICIGEGEYPILELCEALERGADPAGIRNLWVRRNGTIHRNELRPLLSRL
                     DDLPFPDRKVFRFDDLVRDFPEAEFMGSRGCPYPCAYCANHSLLQLYRGHGPYVRYRS
                     VDRLLDEMDAVVRHYPGIEWLGFHDDTFTMSRRWLSEFCEKYPARFQLPFWCNSTATL
                     LNEDTVACLKKAGCYEVRVGVESGNDRLRMDVLRKKVRREDIVRAFRLLREAGIQGYS
                     FNMIGLPHETPGTIRDTIRLNQEIRPSTTFCSVFYPFPGTQAHDVCRANGWLTGRRVA
                     SYFENDYAVNQPTVTRKQVLFYHAIFGDLVRWPRLAPLITLAHRVPVWPGKSLWNAIR
                     RVRAKVVEWGRHLAGCFRPCPVGAEVDHA"
     gene            61892..62881
                     /locus_tag="PK634_00235"
     CDS             61892..62881
                     /locus_tag="PK634_00235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW96191.1"
                     /translation="MLKRAGLVLAGLLVALAFGEWVARWSGRAPEMTLITAGRFRLSP
                     NPAIGYEPAPNFEHHGEKRLYFEFQGRSNNLGFRDRDHTLEKEPAVVRLVVLGDSLTM
                     GLFVDRTDAVFTSVLEQSLAGRGKRVEVMNFGVSGYNTRQEVAMLIEKGLFYRPDVVV
                     LAYCLNDVEEMNGGIMTKLRVEKKEHGGIERSMLSPWLARSALYRVVYSQWLSASAEK
                     ERKEQRMLLKQDTVESSLRLLRETAERNKFRVLVAAFPPRENRRSPERLRRITDLCAS
                     NGFEYLDLQPIFDERAARTGEKLMHDGWHPTTAGHRCAGEAIADGLVRRGLVP"
     gene            complement(62899..65451)
                     /locus_tag="PK634_00240"
     CDS             complement(62899..65451)
                     /locus_tag="PK634_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96192.1"
                     /translation="MTQGRRLLISLAASLAFWTLFSWPLPRHLFEAIPASSQNVEQDH
                     AREMIPGDHLQLLYHFELLHGMLAGRTPWFHNVYEFNLGRDEDRFLPIAYYAPFSLLY
                     AAFAGLAGQAFAWNLAGFVSLWLTYWLTWTLARRFVRSEVTAALAAPIGILLPYRWVC
                     LLGGSPTGFAMLWVPAVWLGLDLAARDGRIAGGALAGAALLMLCWTDIQSLFFCGMAT
                     PLWLLLAALPAGPAAWREPAWRRRILRALAPIALFVALIVLYRWMRQHHLSAADLAAP
                     RTMAEVSNFSPSPAGLVRWASSGLDSHIYVGLLLPLLLLAGLAGLLRRSAWKTPGERR
                     AAVAFLLLGAATVFAVLLSLGVQGPLGGRLLVIIRLLVPPYSLLRQTAKVYCLMPVLL
                     AVAAGLAAEIAGRKLRPKSFAAAFGLLVAGLVFEWRAQVRPTLCVLAGEQKAYAAVAE
                     DARAADELPRALALPLWPGQDAFSSVYEYFAEKHGIRMVNGYSPVPRRAYVRDAYGAL
                     RSLNQGWAPDAALDDLLRRGVRYLLLHENLFPEQVSPFPVTTTLNRLRRHPRLDFLAH
                     ERTVWAFRIRPAPREAAAGEPGLPDGPAVFPARRWELERCSNTLCRAVEDLAASRGGG
                     LALAQAGARVETPSTMASPIPGLRWMIRARGPGLLGATTLVNGQPAGRHDIPVAADAW
                     TWHEAPISLADFSSLALRLDRAEGTVDADLALLAAGDWRDLAPGESRTIAAPCFFHGG
                     YTEPAGNGVVALRKSSDPYAAILYGPKLPLAPGRYAAELTFASPAPAGAELGRLYVEG
                     RDDATSTPVVAGRASARMEWDQLDNLPVNLVFVYFRNADMRIESIAIRRPGP"
     gene            complement(65448..66245)
                     /locus_tag="PK634_00245"
     CDS             complement(65448..66245)
                     /locus_tag="PK634_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96193.1"
                     /translation="MIADTHVHLYRCYDIPDTIRKAIEHLRELARAAQQPDALPVLCL
                     TERHDHHVFDELKSGRLRLDPGKVSALPDEPAGLRIDGGSGEGCAYLLAGRQVITRER
                     IEVLGLAMASFVEDGLPLEDTVARVRAQGGLPVLSWAPGKWFFGRGRIVGAFISRSAP
                     GDLLLGDTSLRPLFWGEPRLMRAGRRRGLRVVSGSDPLPFAEDARWAGRVATVYAPAP
                     MDEARPVAAIARRLLQDTPLRERAGARLCPSQVALRLLRNRKARGMP"
     gene            66452..67609
                     /locus_tag="PK634_00250"
     CDS             66452..67609
                     /locus_tag="PK634_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96194.1"
                     /translation="MNVLFLAPQSFYLDRGTPIAVRLLLEQFSRRGWSVDVLVFPGGR
                     DVVIPGVRFFKTSLRPGARPLQPGLSARKLWHDVRLFFAARERLKTRRYDFIHAVEEG
                     AFIAALLSARTGVPFVCDMDSSMPDQLAEKNRFWRLVRPVMRRFEAYMLRRARAVLAV
                     CEAVADKARAVARGPVFVLPDPPVGGAPNPARNHVRERLKVEGPAFVYVGNLQGYQGI
                     SLLLKSFQRALAHMAQACLIIVGGELMEVEKLERRCNRHGFAGHVHFTGACALDETVQ
                     YLDAADVLISPRIRGANTPMKIYSYMQSGKAILATDIPSHTQVLTPEAAVLVPPQPEA
                     MAEAMVKLASDADLRERLGQAARRLAETQHSRAAYERKADEFCAWMESNVA"
     gene            complement(67621..68664)
                     /locus_tag="PK634_00255"
     CDS             complement(67621..68664)
                     /locus_tag="PK634_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOW96195.1"
                     /translation="MTESIQSLPRGARVLVTGATGFTGQALTRQLAEAGADIRAIARA
                     SSNLAPLADLPVRWFRGDVFEPAVIREAVADVEYVFHVAALYREARHPDEQYWKVHVQ
                     STQELARAVRGRPGFRRFVHVSTIGVHGHIENPPADENYPFHPGDIYQKTKAEAELWL
                     RDFAAKEGLPFSVIRPAAIYGPGDRRLFKLFKMAARGWFPLLGRGKCLYHLIHVEDLA
                     AGLRLAAVHPAAAGEVFICGNPEAIRLEDMGRAIAAALGRRLRVIRLPVGPVFVLADL
                     CELACRPFGIEPPLYRRRVAFYTKDRSFDTRKIREKLGFQYRYSSEAGLRQTTEWYVG
                     AGWLRGAGRPAAS"
     gene            complement(68667..69410)
                     /locus_tag="PK634_00260"
     CDS             complement(68667..69410)
                     /locus_tag="PK634_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW96196.1"
                     /translation="MNERDTTLPDIPETADVETSSPGYAARFAGPAGRWMLGVQERIV
                     LGWMADFPGATVLDVGGGHGQLALPLAGNGYRVTVLGSDGSCCSMIDRQVTEGRLAFV
                     TGSVVALPFPDRAFDVAVSIRLLPHCRAWPALVRELCRVARRAVIVDYPARRSVNALS
                     GLLFGLKRRVEGNTRPFTLFGHDEIARELERSGFAVAGRRAQFFWPMVLHRMLDRPRL
                     SAALESPARGLGLTALFGSPTLLRAERKE"
     gene            69536..70729
                     /locus_tag="PK634_00265"
     CDS             69536..70729
                     /locus_tag="PK634_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96197.1"
                     /translation="MKVLFIAPQPFFVERGTPIRARHQIEVLSRMGHRVDVLCYPFGQ
                     PVDLPGVRVLRTFRPPGLRQVAIGPSLAKFPMDFLLLCKAFGLCLWNRYDVIQAVEEA
                     AFFAVPLKKLFRCRLIYNMDSFISDQLQFSGFVRARPVIRLVEGLERAAMRNASYVVT
                     VGPVLSDVVRRWAPRTKVLQLEDAPLEASFREAPEDARRIREEFALGAAPVCVYTGNF
                     ERYQGVADLVRAAGLVARQRPEARFLFAGGTEAQVAEMRRLADEAGAGSACVFAGRRP
                     TAEMTAFLNVADLLATPRNQGTNPPMKIYGYMQSGRPIVSTRVPTHTQVLDDSCAFLA
                     DPTPEGLAGGILAALGDPDRARAVARAARARVEQRYSLEIFREKVRAAYAGLEAEIRA
                     GRGAA"
     gene            complement(70730..71677)
                     /locus_tag="PK634_00270"
     CDS             complement(70730..71677)
                     /locus_tag="PK634_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96198.1"
                     /translation="MDDLLSLAFLTGVLAAALRMATPILFATLGEIVGERAGVLNLGI
                     EGTMLMGAMTGFTVAFKTGHLWLGVAAAAGVGMLLGLIMAVLVVYLGLSQHVSGLGLT
                     LFATGLAMFIYRLVFGSPVLPPTVEPFRPVAVPLLARLPVLGPALFHQYALVYLSWLL
                     IPAVSILLFRTRAGLRIRTVGENPLAADTVGINVHRTRTVSLVIGGALMGIGGAFLTL
                     AHQNMFLMDVVGGRGWIAIAMVIFGNWSPAKGALGALLFGLLDGLQLRLQAVGLHVPF
                     HVFLIIPYIMTVLAMISVSRKASAPAGLLKPYRREDKGG"
     gene            complement(71670..72755)
                     /locus_tag="PK634_00275"
     CDS             complement(71670..72755)
                     /locus_tag="PK634_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96199.1"
                     /translation="MIRLRLEKRPPLPDWAKAAIPLAAVAATLLLAAAPILAAGGNLW
                     RSYYYLFYGALGSRVNLLESCVKASPLIFTGLGVAFAFRARFWNIGAEGQLMAGAIVA
                     GWLGIALPIPSRLLHLAVIIGAAFLAGGLWAAIPAWLKFRLRVDDVVTTLLFNYIMMH
                     VMGALLFGPLQQPDSSWPQSPEIRATAQYPVLVARSRFHLGIPLALVAAGLVWLIHRK
                     TVLGYESRAVGANPVAAHFGGVPTSSVLLKTAVLSGGLAAMAGVGEVCAVQHHLLMDL
                     SPGYGYSGIVVAMLGNLHPAGVVLAALFFSAVSVGSQTMSRMTGVPSYISEVIQGLAL
                     VVMLVALLLTEYRVRVRRGAGTGGGHG"
     gene            complement(72752..74272)
                     /locus_tag="PK634_00280"
     CDS             complement(72752..74272)
                     /locus_tag="PK634_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96200.1"
                     /translation="MNTDPTPLIEMRGITKRFLGVPANRGVDFRLEPGEICALLGENG
                     AGKTTLMNILFGYYACDEGEIRIGGERVNLSSPRAAVRRGVRMIHQHFALVPSLSVLE
                     NVAVGQEDHRLGFLDWKAARKRLLELERRFGMTLDPDAKVWTLSVGEQQKVEILKALY
                     RECRVLIMDEPTAVLVPSEVRELFAMLRTFAAQGNGVVFISHKLHEVMDISDRIVVLR
                     NGEVTAEKQTRDTNARELAGLMVGRDWPARKERSPRAPGRPALEIVGLSATNDKGLPA
                     VRNLDLAVREGEVLGLAGVSGNGQRELAEVLFGVRKPTAGSVRIGGAPTRPGSPTAVI
                     AAGAARIPEDRIATGLMMDLSIAENLILENRRSAEFRRRGLLDHGRIREHAGRLIGQF
                     GIKAAGPSTAAKSLSGGNLQKVILARELSAGPRLIVAAQPTRGLDVGAAEFIRGRMLE
                     ECGRGAAILLISEDLDEILSVSDRVAVMYEGRVVGIRAVNEITREQIGLWMSGVVP"
     gene            74390..76966
                     /locus_tag="PK634_00285"
     CDS             74390..76966
                     /locus_tag="PK634_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOW96201.1"
                     /translation="MKTRGAPFCLLSVLGLLTLSAFGGTTPQKVFMLMDTEAYQALTN
                     EVNQWKADVNAEGRYQAEAYAIPPDNPALLRLLLALHYQGGNLAGVILAGRVPYVMCE
                     QDSEYLVTPDAFPVDTYFTDFDAEWYDLNLNGIMDSYYAFGGFQPDIWAGRLFTDTIK
                     FNSKTRIDLLRAYFQKNHQYRAGTLTLPKSALVYYDIKNYTTANDFGKLYPTFQTVWP
                     DTSSGTDYLNRLLPLPGNELVYLDCHSSAKTHQLTPTWIASDLIHAVNPKAFFLLLDT
                     CQSGHFTETNYLAGSYLFDTKYPLFAMAATTGSGYNFPTGFQTLSAWAGEDWGSIFMH
                     FAREQSWSREYILLGDPTLIPHLNPPQVALSLPYAFSSAAPLKIPRPASGAAQEWRIP
                     IRGKLTVGAGNWLRVTAANAPAGMAPRTNDFHLTVMGLTNAGDATVAVTPTTTLGKRQ
                     LTVVARDSRGKTAALPYWIEVVAPPATGKPDFTFTVGDTNLVFRYGEGDPFGRLTVQA
                     QLTTADLSAPMLVNLVPVNWPDGFSLDNQTSSPESWSGGFLLQQKGVTRYTYVTVRMR
                     RDMAPGQYVLPLKARAMGVEKTVNLTAAVLPPSLEVTTVPSPVYILPGDEASIFSTVA
                     MNGNARVYFDLLSYPGIEVQKVLTTMPFVPYGGHSVHVWTDWDAASGAYSLLRYQFPD
                     MPGAPVGYGLDVVVRPQFTLDIELVSGGSGGGVLQVTMHNQAVGNPAYPLTVNAESDA
                     VAEVNVLNPQVTMPAGFGAEAHALIEFSTPVALLPPGGHSIYYTITIRVTSPMQEWPE
                     QECALTLKLMAPPAAPTEWEIQPVHFIPGPDPVPCELDLPVLIYGNQRFTATPLVATR
                     LR"
     gene            complement(76977..77978)
                     /locus_tag="PK634_00290"
     CDS             complement(76977..77978)
                     /locus_tag="PK634_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOW96202.1"
                     /translation="MKPLRMIPAALILALLASCSPPAPPASEGAKAFRVAAVFQTAIE
                     EPWDGVIHQACLKAKEELGVEYEFTEKVAAADYEKVLREYADRGFDLIVGDAFLAGED
                     PVRRVAKDYPEIAFAFGSEFGPAAPNLSVFDNWIHEPAYLCGVIAGKRTKTGTIGAVA
                     ALPIGEVNRLVNAFREGARSVRPDVKMKVSYIGSWFDPPKAKEAAIAQIEAGADLIFA
                     ERFGVYEACKEKGVLAFSNMYDQHGMAPETIITGPVWDMGPTIRHCIETVRKGAWVGM
                     DLKDWSMMAQGGARLAPLHDFEARLPADVLASVRDLEAKIVQGAFRVPVDESKTVSD"
     gene            complement(78269..79243)
                     /locus_tag="PK634_00295"
     CDS             complement(78269..79243)
                     /locus_tag="PK634_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOW96203.1"
                     /translation="MARPPRNEFENALYHVTSRGNARQVIFHTDGDRRRFLLQLQDNL
                     EQYGVVLYAWVLMSNHYHLVVRTPRANLGRFMQRLNSSYSLYYRYKHAKTGHAFGGRY
                     KAPLVESGDYLLALTRYVHLNPVKTRAAKEKTAKERIRMLEEHPWSSYPDYVGSRRTA
                     SRIDLAVLQHYGRSREEAQRRYRAYAQAMVAEDDEPLKGLMARSTHAWGSEEFVEKVE
                     KELLGGKTGRARDADVQRPWRGVEMAVLDEVVARAYGIEAGTLKRHGRSAGEAKAVAV
                     ELASRWSGRTQREIGAHYGGISGQAVHMIRRRLIDKKIDWPKLEEALA"
     gene            complement(79341..79721)
                     /locus_tag="PK634_00300"
     CDS             complement(79341..79721)
                     /locus_tag="PK634_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96204.1"
                     /translation="MKKWKIFAGIALIFAAGFVSGITVTRGVVRRRVAQWATWNPEAT
                     RRAVLAHLTRELKLTPRQQDAIEPILAALQQDLQHVRSQLQPEVHRIVVEKAREMAPY
                     LTPEQQTGLRLFLLQQRARWTDPK"
     gene            complement(79735..80067)
                     /locus_tag="PK634_00305"
     CDS             complement(79735..80067)
                     /locus_tag="PK634_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96205.1"
                     /translation="MKEKQRIEHIETALLAARHLPPAHEPAPDWQDGVMREIRRIAPP
                     EGQELWTAPIETPLRWMVPACAAALVLLLGLNLYWNRTSELDRIEMVLTDPAAPPLTR
                     ALWLRSIL"
     gene            complement(80082..80723)
                     /locus_tag="PK634_00310"
     CDS             complement(80082..80723)
                     /locus_tag="PK634_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOW96206.1"
                     /translation="MRLDSTITKGIVGEREMFMEGAEIETDDLETIRRILEGRVDEFE
                     RLLTKYRARVFSIVLNHVPPDHADEVAHETFIRAFRSLAGYSRRHPLEHWISRIAVRT
                     CCDYWRRRGRCPEIPVSALSEEHRAWLERAGDGGSKAECEAAAARREAAEVLDLALAR
                     IDAGSRAIVVMLYAEGRTVSEAARLLNWSAARVKARAHRARHALRKIIRPWIR"
     gene            81130..82371
                     /locus_tag="PK634_00315"
     CDS             81130..82371
                     /locus_tag="PK634_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96207.1"
                     /translation="MNTDFRKTVPGGGWGGVPAFLVVACGVMILLVRTGYAQSPFEGE
                     WQLQLIHRGGLYPKWSKANNLIVFNELRPGKAFTDRLPGTYEIFTMKPDGTSVTCLTD
                     KAPVGLSGFHKCQPYWHPSGRFIVFTAQNEHTTPTEHNMDSFPGIGHNHDVWIMTADG
                     RRYWRITRNPDNWGVIRPSFSHDGKKVYWNEEYSMEAHPGMGSPWKKERNPKGEEWGL
                     WRIKMADIAFSPNGPRLAPVRIVPINHLHPGFRLIEGSGFTPDDGHLIWEAAHLGETD
                     GQMWWGDVYVSDLKGGSLRRLTRTAPLRRGNENMEYSPDGQSIAWSYHDNPEPGKKVE
                     LWLMRSDGSDATPLTHFNTPGHPHRKRFQPLRFTNACLELDWGPDGQAIVFSMSNGAT
                     LEWPYMTPNIYLLTMSGDITK"
     gene            82479..83582
                     /locus_tag="PK634_00320"
     CDS             82479..83582
                     /locus_tag="PK634_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96208.1"
                     /translation="MAGHWRSGTASGLIGRFGRMFPVMVSLGCAVAGAGAGEDPHRPA
                     FHVGYQILDLPCRKEGVEHTLTVAVWYPTAARPRPHHYGGLTRGRVAVDAAPDAERGP
                     YPLLVFSHGYGGSGLGVVFLAEALAARGWIVAAPDHHDRHSAVRIREGPVEDYDRRGL
                     LRHSMRIAASGRHDRADYMYRLDEMQLVLDRLSDSEPFGRLTDPNRVAVGGHSFGGFT
                     ALGVCGTIPERFDPRIKAVLLFSTGAGGYLFSDDELAAVRIPSMLFLGELEKESARGS
                     SGTMAELSGKVYRNLPVPKYFLEVKGANHLSFNNSFAGRTSSRPGADDSRIFDVIRRY
                     SIAFLEKHVAARGDDGRVLERGEAMLTRYLKEP"
     gene            83651..86047
                     /locus_tag="PK634_00325"
     CDS             83651..86047
                     /locus_tag="PK634_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOW96209.1"
                     /translation="MNKRVAVLAFAGHSPCAAGLNSGGGRPSSALRCAIMPFGQEQLF
                     EALAIRVPRRRAAPYFFGDNVEGYFEGQTHRTAEGAGYLLHGRALFRDFLSWRGELFN
                     ERTKAEGAAIYPYGIRHDHGPSWWDELMLLRRQQAVALRAFSRQPQTLALAPLLDLAP
                     RHLVVQAKPWGGFITDRKKTFCVGVSSSQPFRHDGTVTRGRLVAPVFRTLQDESEFTL
                     YLAFDRDPRRAQGRVLALREADGVRRHKAEIFGLLTRSHLWTDDEDYNRALMWAKLSS
                     LFLVENEFGQGIWAGLPWFKNNWGRDTFIALPGTLLVSGMFDEAREVIRNFVRWQNTD
                     KDSPDYGRVPNRVTGTRAADKIYNTADGTPWLIRELYEYLCYTGDRAFGEEVYPFVRT
                     AIAGALKHHGDKQGFLTHADADTWMDARIEGKEPWSARGSRAVEIQALWFNALLAGAR
                     LADLVGDAKSAREWALLAAKVRKSFAKKFWDARGRRLADRLRPDGTADFKVRPNQLMA
                     ISVPMIEPLVDDATAARVTANACGELLYPHGIGSLSQDDPYFHPIHHREESYHFDAAY
                     HNGIVWGWNAGPAVTALCRCGQTELAGQLARNLAAQILEQGCRGAMSELVDAWPDAKG
                     KLVLSGCWAQAWSSAEFARNGYQDFGGFEPRLLDGRIVLRPRFPAAWTRFAATFPFGR
                     GASLQLTYANEHGKEVFVLTLDGKTDPLTVSLDAVAGGFRFVADREMKSADTWMLVIE
                     KGQALSGLNGRWEGAPVKGRKQGRARPLGFVRLDPRAKPPSLKKKDYLKDIILAGRYR
                     "
     gene            86281..86433
                     /locus_tag="PK634_00330"
     CDS             86281..86433
                     /locus_tag="PK634_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96210.1"
                     /translation="MKARYQKPEIRKIKLVPSEAVLQACKTRGASGPAGGSCLAMNGI
                     CSAYGS"
     gene            86497..87753
                     /locus_tag="PK634_00335"
     CDS             86497..87753
                     /locus_tag="PK634_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96211.1"
                     /translation="MIRIGFITPASGPAEQKSVDRVWRRLEEALAAEFELVRYPGAAE
                     GVAWRAFLDRVEAVVVPHPIRADRSGSLRKPFLHLALGTLPLGGLRFLRMLADLSNAD
                     SILISCRADQAIVERVFPRREIGASFLPFGVDLDVFRPLGEAAREAARAAHGLQAAAP
                     VLVYAGRVTRPKNVHLLLDMVAALRPEFPGIRLVVAGGPEPLDSEADSYREELDARAR
                     DLGGAVVFAGPLDDTRLAELLSAADVFVTATVNRDENFGFAAVEAMACGLPVVGTEWG
                     GLKDTVAPGETGVLMATRVADTDVRVDAPAGVAAIRRLLADPALRRNMAERALARARE
                     HYGLDSFRRHTVRIVREAIDRAAGIGEAIPGTRFELHPLAMESLVYSQLGVQRDWYGR
                     VPGFTPEDARLRRFFMEPYASGSEPR"
     gene            87750..89024
                     /locus_tag="PK634_00340"
     CDS             87750..89024
                     /locus_tag="PK634_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96212.1"
                     /translation="MKKVLLIAFEFPPAVASGMYHAFYLAKYLRSFDWDPFVLTGVPG
                     AVPAAMEDPLDLPAALDEGRVLRAGLADEAELAEFAGRELPDSWLRRCVLGHRDEHGG
                     SFRHVFPDPLVRWLPRAWRRAVELLSAHRFDAVVTTSFPFTSVLLGRMARERFGVPWV
                     AEFRDLWTEDPRFPLRSAAFVADSGRLERETLASADAVVAVSSPLAERFVRRGGGDRV
                     RCIELSYEEADFAQEREPPSDRFHLVQAGTLYPGMLPAALPKALDLLARLGKLDPARF
                     GFTLAGANLCGQAGLSGARVLGHVPHAEALRLYRGASALLLTLPAELGEFIPSRLFEY
                     MASGAPILALVPPDSVAARMVLRSRTGTVVDIAAGVDEVAAAVYDLYVRGRDGTLTTD
                     PDWAYIRGFSSRAMAGRWAALLNGLRAGERVQ"
     gene            complement(88989..90191)
                     /locus_tag="PK634_00345"
     CDS             complement(88989..90191)
                     /locus_tag="PK634_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96213.1"
                     /translation="MKKVLVISYLFPPRGGVGVIRIAKFCKYLPEHGWRPIVLTTEAP
                     AEMSRDAELLAEVEASSVLVRMPLPPGGRRRGFPEVSIRWRRPAIAQALRLAREEGVA
                     AIFSSGDPVVNHLVAGEAARRSGRPWLADFRDEWAVNPEFIRSPFQRARADRWERDIL
                     EKADAVVSVTAPVVDMLVEKGGGPAAKYHLIPNGYDGAVLSALPSAPARRDRFTITFT
                     GHLPNPADMLPVLKALRLMLSLRLAREDELLLRLVGDFEHFRHDEAMGLSGLVRKIPP
                     GSHREALLRQQDSDLLLSVSDARRGRYAVSVKLYEYMASGRPVLAVAPAGSLMADYVA
                     RSRTGRAADPARPDEIADAIAYFFARWKRGNLAVEPDRAFLEHFDRRRTAGQLAGLLD
                     SLAGAQSV"
     gene            complement(90222..90575)
                     /locus_tag="PK634_00350"
     CDS             complement(90222..90575)
                     /locus_tag="PK634_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96214.1"
                     /translation="MNTEHPPRELEPDAETLLLSNEVQLLLSEKRTWLSVLRTGIAVF
                     ALPLSVLSILITTSRLYDVSKVIVFLTLVLTLSFGLVVLGAYLVARAMKKLRALDRHI
                     LKLKGRSRIIADLVT"
     gene            90705..91733
                     /locus_tag="PK634_00355"
     CDS             90705..91733
                     /locus_tag="PK634_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96215.1"
                     /translation="MPVAIVTEGLTKRFPQKVSLRQAALHPFRRLSGAPLTAVDRVSL
                     EIRQGELFGLLGPNGAGKTTLVKMLCTLVLPDSGRAEVNGFDLSDEARVRASIGLVSG
                     EDRSFYWRLTGRQNLEFFAALHGLSARAAAGRIARAAEWLELGEFLDRRFDRYSSGMK
                     QRLGIARGLLNDPSILFLDEPTRSLDPVSAAHLRDVVKQLVRERGHTVVLVTHQLHEA
                     EDLCDRIAIMHKGRLRVVADPLTLRRQAGVRHYRLRVRGLNAEGAALVKGWPGLAIRE
                     PAGDPGLLVLEFQPAEGEAGEALANLLDRVVGAGGRIEDVETPEAALETAFRKFTRDA
                     APAAEAGS"
     gene            91730..92542
                     /locus_tag="PK634_00360"
     CDS             91730..92542
                     /locus_tag="PK634_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96216.1"
                     /translation="MTGWRKPLAFVKRDLTVLISYPMAFFLQFFGIFATILMFFFVSR
                     VFGGAASPHLAEYGGDYFSFVLVGLAFSGYMTVAMNSFAASIREGQMMGTLEAMLVSP
                     TRLSTILISSSLWNYTFTSLRVVFFFLIGALCFGVDLSHANLSAALVVQGLTILSFSA
                     LGIVSASFIMVFKQGNPLDALSGVVFSLLGGVLYPVAVLPAWLQPVAWCLPLTYSLRA
                     MRRAMLRGASWGELLPDILALVLFAAVLLPASLYVFRRAVRRARRDGTLTQY"
     gene            92651..93226
                     /locus_tag="PK634_00365"
     CDS             92651..93226
                     /locus_tag="PK634_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOW96217.1"
                     /translation="MNATLFQRISWLAGRALGSLPAAALLKIALPLRPVVRDVDPVRD
                     LDALALFGAALRPALRPRLRAAWEQALRDAAPDFRRVLVAADARRPDRLAGFLHLQKG
                     RPDWWIAGMEVRPLYRRRGLAELMAREAIRRLKAEGARTICLSVRAGNASAIRLYEKL
                     GFRPAEGLSDPTLHPGDVGMRLILQPGAATG"
     gene            complement(93195..94454)
                     /locus_tag="PK634_00370"
     CDS             complement(93195..94454)
                     /locus_tag="PK634_00370"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOW96218.1"
                     /translation="MNPPADQHREARFLAQIARTRPADADIAAARETAAGGLDPLRLL
                     PLVDQNRLAPLLYRNLKAAGLDAVLPPGTMSALQQRLLQEMLRGARLEQETRNLLERF
                     QARGLRVILLRGLAVGEAVYGDGSLRPYSDLDLLLLKEDLPAAKEQLREAGYGAPAKA
                     LEDGYYERQHLHLLYVHRKTGVMAEIHWALDHRFTPWLVDYAELFRDARPGTIAGAPA
                     LALSPEDMLLSLGLHLVKHGPCAELLLDRPAAAERILAAGWWIHVLDVAEAVERGRET
                     LDWARVADKARRWNIGAAVRASLRCAGQLLRAPMPAGLIESLGAPRASGLARRLQEWQ
                     LRALAPDAAKPSGRLAGFRHDIAFRPVRLLDALLYLWPDEAYLARRYPERSPFTRRGR
                     HLAGGIGELARGFADLLVSQWRRRAAG"
     gene            94585..94875
                     /locus_tag="PK634_00375"
     CDS             94585..94875
                     /locus_tag="PK634_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOW96219.1"
                     /translation="MSKEKARMAKTGDVVRRQIADEIILVPVRKSAEELDSIYSINPL
                     AAQIWDLLDGQKTLGEIRDILLQQYDVTPEELDRDLADFVEQLKAEKLITEA"
     gene            94877..95980
                     /locus_tag="PK634_00380"
     CDS             94877..95980
                     /locus_tag="PK634_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96220.1"
                     /translation="MTESRVPEVGYEYFSERVHNQSLRKRLPLSGSIELTFACNFRCI
                     HCYCVEEPPRRELSLEEIRGLVDQVADEGCLWMLLTGGEPLLRRDFADIYNYCRRKGL
                     VTTLFTNGSLVNERIAGVLSDLKPFVVEITLYGFSPETYRAVTGRAENFEKVLRGIDL
                     LQKAGVPLRLKSMILQQNKGDLPRIREFAQSRGLNYRFDPVLNPTLSGSRKPEELMLT
                     PEEIVALDVGDPERLKSWDEFCEKFTFASDDGRLYKCGAGQWSFHIDPEGKLSLCMFA
                     RQNQYDLRSGNFHEAFYTFLPTVLGQKESRVTKCGACPIRNMCGQCAGWAHLYSGDEE
                     EPVEYLCRIAHARANAFGLNKIGSSAPPASGNA"
     gene            96059..96211
                     /locus_tag="PK634_00385"
     CDS             96059..96211
                     /locus_tag="PK634_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96221.1"
                     /translation="MKKTYKKPEIRKVKLVPSEAVLQSCKTTGAAGPGQQSGCFITLI
                     CSIIGS"
     gene            96320..96475
                     /locus_tag="PK634_00390"
     CDS             96320..96475
                     /locus_tag="PK634_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96222.1"
                     /translation="MMKKAYRKPEIRKVKLVPSEAVLAACKTQGVSGPDVSGCQPVSH
                     ICASIGS"
     gene            96555..97418
                     /locus_tag="PK634_00395"
     CDS             96555..97418
                     /locus_tag="PK634_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96223.1"
                     /translation="MVQKAVFDIAGITYVIEKAPGGAELDVGPSHQHFLSDKEPALRF
                     RAVDGPWPSLEGLKKTFDSTSWWSMSEAPGARVVSFFNATPPYEENRRAVLAPDYRSG
                     AVYCRNALDQSFPLNYPLEELIYINVLPSFDGVIIHSCGAGDGNGGYLFVGVSGAGKS
                     TISKVLMKGTDWKVLSDDRIILRKMEGGIRMFGTPWHGEVDVCENMGIPLRRIYFLKK
                     APRNEAVRWAPALAATHLIARSFPPFWDADGLDRTTRLCIDIATQVPCYELGFVPNAS
                     VLDCLAGLKDG"
     gene            97465..97989
                     /locus_tag="PK634_00400"
     CDS             97465..97989
                     /locus_tag="PK634_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96224.1"
                     /translation="MADSRDIQRVLLAEGNEVMLRTAGLSMGHAIRGGEAVVIRAVPE
                     RDIRFGDILVFERGDQLVCHRVVGTRARGAGREFVTKGDPVLGLDEPIPYAAILGRVV
                     RVKWPGGDFRLDTRRGRWSNRFLAVVSLGVAGLYRVFPWKGALKKPGSGVHAVWRLLA
                     FPSRVVRWISERCR"
     gene            complement(97993..99327)
                     /locus_tag="PK634_00405"
     CDS             complement(97993..99327)
                     /locus_tag="PK634_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HOW96225.1"
                     /translation="MPSDEPHRGDRELLFGLDDRPPFWIALFAALQHLLAVFVPIITP
                     PLLICGALKCDVPTTTTIVGLSLFISGVSTWIQVRKVGPLGSGLLSIQGTSFTFVGAL
                     IAIGQTGGLPLIFGVCLAGSAVEMIISRFIPLVRRLIPPLVSGIVVTLIGLSLVGAGA
                     FNCAGGGAAMADGSFGHLRHFALAGLVLAVILVLNRSRNALVRMGSIVAGLLAGYAVA
                     ALLGRVDFRPIAELDRVVLPLPFQYGLAFRWSAFIPVALLYVVTSIETVGDITATSIV
                     SRQPIAGPLYLKRLAGGMLGDGFNSALASVFNTFPNTTFSQNNGVIQLTGIASRTVGH
                     WIAGLLVLLGLSPLVSGVFALMPEPVLGGGTLLMFGTVAAAGIRIIASQPLDRRALLI
                     LAASLGTGMGVVLAPDALKALPEWARHVFASGAVTGGLTAVLCHALLPAARE"
     gene            complement(99333..101030)
                     /locus_tag="PK634_00410"
     CDS             complement(99333..101030)
                     /locus_tag="PK634_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOW96226.1"
                     /translation="MAWFPRGTWRYAVMILLLLVLAAVAAMSTLSSIEKFLLTDARSA
                     TPEALRSYLGIITLTILALTMGFLFLAGAMGVWAIRSTAQVESRRRVGRFVDAMEYLS
                     DGLLAVDRRGRITGSNPAARRFAGAEPDSKSRLRDLFTALSAEQESLLLGGREPQEVE
                     CLVREAEGLRALRFRSQPSEDVQLILVSDVTQRKAEEIRSRQMATLQLIGRIARGVAH
                     DFNNILCAISGHAELAGRGASGPDQAASLQAIAHESRRGASLAAQVLRLSQAEARGQP
                     CTRLADQLQRATQLLTVGLSSRWRVAVDIQGGLGPTLLTGNQIEQIILSLALLAADEL
                     GHPGHLHIRARPPDAAAPLFDVGTRCAAAVWIAASEAEDASSAPVKAIPLEAGVVVSV
                     VRSILGEAGGRLDVLAQEGRHGYRLCLPSLPVAEKSMALGALTAEEALLVSMWKILVA
                     GARTTARDDWARLFRDLNMEVRTADDIVATLQAVEAPGDLAALVLERKLLGEEAPALL
                     RAIRKLRPRSGLILLTDDQSSWDPALGIEVIRETAPGAETLLRAMLRSASARQGTGA"
     gene            101169..101978
                     /gene="dapF"
                     /locus_tag="PK634_00415"
     CDS             101169..101978
                     /gene="dapF"
                     /locus_tag="PK634_00415"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOW96227.1"
                     /translation="MRIPFWKMHGAANDFILADDRAGTFPAGDRGWLARIMARRTGVG
                     AEGVLLIQPSARADFRMRFFNPDGGEVDMCGNGARCIARLAHELGAAPREMRIETRAG
                     LVRAAVDGDSILLHLTEPRDWQLNRQLEVAGQILDAHSVNSGVPHVMVEVPDLDAVNV
                     QELGRALRYHPAFAPGGTNANFIQVTGPHSLRIRTYERGVEAETLACGTGIVACGLLA
                     GRLGRVRPPVSILTAGGDTLEVNFAPAADGATGVTLRGPAVHVFQGTLEYP"
     gene            101975..102835
                     /locus_tag="PK634_00420"
     CDS             101975..102835
                     /locus_tag="PK634_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOW96228.1"
                     /translation="MKASPVQSRTVLVTGCSTGIGLATARLLRERGWAAWPTARKAED
                     LARLRAEGFNPIELDLYDPASVCRAAEEALKRGGGRLGALVNNAGYGQPGAVEDLTRE
                     ALRRQFEVNVLGVQDLTNLVLPVMRGQGYGRVVNVSSVLGRLTLPFLGAYCASKHALE
                     SLSDALRVELRGTGIAVSIIEPGPIETAFRKNSFEQGQERLDISKSRFAERYRSEGSR
                     RAAKNFRSSDPFTAPPEAVARRIVHALESGRPWRRYPVTLPACAGSLLARFLPHAIID
                     WLLSRRMGKS"
     gene            102953..>103225
                     /locus_tag="PK634_00425"
     CDS             102953..>103225
                     /locus_tag="PK634_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96229.1"
                     /translation="MKKHLAIIVALVGLIAPSLAATETISVSMDMPAVSIGNETGGFV
                     DVLAEGRNSDGRPARPMLPGKSVFYALPPDVDAASVSIVVTKTEYRD"
BASE COUNT        16751 a        34219 c        35564 g        16691 t
ORIGIN      
        1 caccggccct gttcgcctgg acgaccggcg catgcgcgag atgctgaccg gcggcgcgcg
       61 ctgggccgtg gggcaggggt tcggctcgga gcgcgacctc gaacgcatcg agtcgcgggg
      121 cgccgtcccg ggcgcccggc cggaggacgt ttccgaccgc gcgcgcaagc gccaggaaga
      181 agagatgggc acgctgggct ccggcaatca ctacctggaa gtccaggaaa tccgggagct
      241 ctacgacgaa gctgtcgcgg ccgcgtttgg cctgcggaag ggcgaggtcg tggtcagcat
      301 ccactgcggc tcgcgcgggc tgggtcacca ggtcgcgacc gattacgcca agcgcatgct
      361 cgacgccgcg cggcggcacg ggttgagcct gcccgaccgc gaactggcct gcgcgccgat
      421 ccgttcgccg gagggacagg cctacctcgg cgccatgcgc gccgcgatca actgcgccct
      481 cgccaaccgg cagctcctcg ggcacctcgt ccgccaggcc tttcggtcgg tcttcggcga
      541 aaaggaaatg ccgctgcttt atgacgtctc gcacaacacc tgccgcgagg agacgcacga
      601 ggtggagggc gccgcgcgga cgctgttcgt ccatcggaag ggtgccacgg gcgcgctcgg
      661 gccgggccac ccggatttgc cggacgacct gcgcgccgcg ggccagccgg tgctggtcgg
      721 cggcacgatg ggcacggcct cgtggattct cgccggcgcg cgcgcgggcg ctacccgcgc
      781 cttcgcctcc gcctgccacg gcgcgggccg gcagatgagc cgaacccagg cgacgaagca
      841 atggcagggc cgggaggtcg cggacgactt ggcgggccgc ggtgtgctga tccgctgccg
      901 ctcgatgcgc gggctcgccg aggaggcgcc cggcgcgtac aaggacgtcc gccgcgtggt
      961 cgaggcgacg gtggccgccg gcctcgcccg caaggtggcc cggctggcgc cgttgatctg
     1021 cgtgaaaggc tgaaaagcgc cgttcgcatc accggcgcat gtatcacagt ttataaactg
     1081 tgatacatgg cattgtgggc cgaccttctt cctttgcgtc cttcgagcct tctgttaaac
     1141 tctcttcatg ccgacccaca acgccgatat ctcggcggtt ttcacggaga tcgccgacct
     1201 gctcgatatc cagggtgaaa acccgttccg gatccgcgcc taccgcaacg ccgcgcgcac
     1261 ggtccaggac ctcggccgcg agctgcgcga catggtagag gccggcgagg atctgaaaaa
     1321 gctgcagggc atcggggacg acctggccga gaagattgtt gaactggtcc agaccggcgc
     1381 ctgcaccgcc ctcaccaagc tgcgctcccg gttcccgccg ggcgtcaccg acctgcttcg
     1441 catcccgggc ctcgggccga agcgggtgaa ggtcctgtac ggcgacctga agatcgagtc
     1501 gctcgcccag ctcgaggccg ccgcgcgcga ggggaatatc cgggacctgc cgggcttcgg
     1561 cgagaagacc gagcagaaca tcctgcaggc catccaggtg aaggcggaca ccgggcgccg
     1621 ctttctcctg gcccacgcgc ggacctacgc cgaggcgctg accgcctacc tggaaaagct
     1681 gccgggcgcg ggccgggtga ccgtcgcggg cagctaccgg cgcggccgcg agacggtcgg
     1741 cgacctggac atcctcgtga cggccgagtc ggccgggacg atcatggacg cgttcgtcgc
     1801 gtacgacgag gtcgcccagg tcctggccca cggcgacacg aagtccgccg tcgtgctgcg
     1861 ctccggcatc caggtggacc tgcgcgtagt cgaggagaag agctacggcg ccgcgctgca
     1921 ctacttcacc ggcagcaagg cccacaacat cgccgtgcgc aggctgggtc agcagcttga
     1981 cttgaaggtc aacgagtacg gcgtcttccg gggcgaggag tacctcgccg gccgcacgga
     2041 gaaggacgtg tacgccgccg tcggcctgcc gttcatcccg cccgaactgc gcgaggacgc
     2101 gggcgagatc gaggccgcgc gcgaggggcg gctgccgaac ctggtcgaac tgaagcacct
     2161 gcgcggcgac ctacacatgc acacggacgc ctccgacggc aagaacaccc tgcgcgagat
     2221 ggccctcgcc gcgaaggagc gcggctataa atacatcgcg atcacggacc attccccgcg
     2281 cctgaagatg atcggcggcc tggacgggcg gaaactggcg cggcaggcgg acgcgatccg
     2341 ggaactgaac aacgaactgg agggcatcgc gatcctgcgc gggatcgaag tggacatcct
     2401 cgaagacggg gcgctcgacc tgccggacgc ggtcctcgcg acgctcgatc tcgtgatcgg
     2461 ctcgatccac agccatttca ggctgccggg ggcgaagcag accgagcgcg tgttgagggc
     2521 catggaccgg ccgcatttca ccatgctggc ccacccgacc ggccggctgc tgctcgatcg
     2581 cgagccctat gaactcgacc tgccgcggat catccgccac gcggcggagc ggggctgctt
     2641 catcgagctc aacgcccacc cgctgcggct cgacctcgac gacacgttct gccggatggc
     2701 gaaggaggcg ggcgtgctgg tgagcatcaa cacggacgcc cacgggatcc ccgaactcga
     2761 ccacctgcgc tatggcatcg gccaggcccg ccgcggctgg ctggagaaga aggacgtcct
     2821 caacacccgg accctgaagc aactccggcc gctgctggcg aagaccatgg gacggtgaca
     2881 gggccgcaac gtccaacacc caacttccga cgatcaacgt ccaaggccct ggaagttgga
     2941 cgttgggtgt tgaatgtcgg acgttacgtt cgttccggcc cttcttgcct tgccgcccgg
     3001 cctgtgggaa catgcgcgcc ggcaccggca ggacggcgat gacccatgcg atcaacagtc
     3061 tggcggcggt ggtcttcgat ttcgacggcg tgatcgtgga tacggagccg ctccacttcg
     3121 aggcgttccg gcggatcgcc tcgccgcacg gcatcgagct ggcgtgggag gcgtaccggc
     3181 agaaatacat gggattcgac gaccgggacg ccttccgcgc gttgttccgg gacgcggggc
     3241 ggccgctggc ggaagaacga ctggccggac tgatcgacgc caaggcggcg gcctttgcga
     3301 agctggccga gagcgagggc gtcgagccct atcccggggc ggcgcgcctg atcggggaac
     3361 tggcgggccg cgtccccctg gccctgtgca gcggggcgct ccggtccgac gtccagccca
     3421 tcctggcgcg ggccgggctc aaggcctgct tcgctgtcat ggtgacggcg gaagacgtct
     3481 ccgtaagcaa gcccgacccg gagtcctacc gggaggcgct tcgccgcctg gccgctcggt
     3541 tccccgatcg aacattcccg gcgtcccgtt gcgtggcgat cgaggatacg ccggcgggca
     3601 tcgcggcggc ccggggcgcc gggctgaagg tcctggcggt gcggacgacg cacgcgcacg
     3661 gcccgctggc ggcggccgat tgcgtgcagg agtccctcca ggctctgcgc gtggaggatc
     3721 tggaggcgct ggcccgggat tgaacgcggc gtttgcggga ttgtcttttt atcccacggg
     3781 cgataaggtg gcggctcgac ggcaacgtgg aggaagcgat catgaaaaag acttgggcgt
     3841 gggcagccgg gatcatcctg ttggcgggca tctcggcccg ggcgcagggg caggccggaa
     3901 atccgtacta cgtggacggc atgctcgtgg accagtacaa gcagtcggat tcggaagaag
     3961 cggcggcccc ggtcgccgcg ccgaactcga tgtccttgcg tgatgcgccc cccgcaggcc
     4021 cggtgcccca gccgtccgtc gctcctgcgc ccaaggcgcc cggcgtggcg ctgcccccgg
     4081 cgggcaaggc cctggagcat ctccgcgccg ccaacatgtt tgtcgagaag aaggaatggg
     4141 acaaggcgct ggaggaaatc cagaaaggcc tcgaatcgga tccgcagaat gcgctgctcc
     4201 tccggcgcgg atcggccgtc gcggcgctgg ccaagaatta cacggcggcg gacgattatt
     4261 accggcggct gctgatggtg gacccgaaca gcgtggcctt catgaacggg cgcgcctcgg
     4321 tgcagatccg cctgcgccag ttcgtccccg cgcagattct gaccgaccgc gccctcgagc
     4381 tgcagcccgg cgacctgctg gcgcggttca accggctgct gctgaaggtg atccgcaacg
     4441 agccgctgga gggggaggcc tggaacgacc tgctcacctt tgacctggcc caggtggcca
     4501 actggctgga ggcggacaag accgagtacc agcagttgat gtcggcggag cagttcgcgc
     4561 tggcctgcca gaccgccctg ggcgcaggcc tgggcgacaa gctgtccgat gtggtgggcg
     4621 tcttgcgccg cctcggccag gcccagcggc tgcagcagtg ggacaaggtc ctggcgatcg
     4681 tgcaggaggc ccgcgcgctg ggcctccagg ccatggggct ggagatggac caggcgcggg
     4741 cggtcatgga gcagggcgat cgggaccagg cgcgggccat catgaagggc ttgagcgagc
     4801 ggttccccga gcacccccag gtcctgtaca actacgggta catcctcatc gaggacagca
     4861 actacacgga ggcgctgggg ctgctggacc gcgccttcgc gcagattccg aaccatcccc
     4921 agatatcatt tgcccgggcc tgcatgctgg ccgagaacgg ccggatcgac gaggcctggc
     4981 cgatcctgac cggcctgatc cgcgactacc cggcggagac gaggacctgg ctgcaggagg
     5041 aggatcccta cctggccaag atcaaggcgg atccgcgcta ccaggaattg ctggccaccc
     5101 gcgtcccgaa gcgccgcccc gcgggacaag agcaatagaa caaggagaac gatcatgaaa
     5161 cacatgctga tcggctgttt gtgcctgttc gccacggcct gggcccaggc cgcggatccg
     5221 gcgatttcgc tggtggtcgt cggcgatctg gacccggcga tggcggagcg cgtgcgcgcc
     5281 ttcgcgcagg agaacctggc ccttccggtg cgcctgcttg agccgcgcga ggcggcggcc
     5341 gcctccagcc tcaacgagat cggcgacgcc gcggccgagg cgatgggccc ggaggatacg
     5401 tgcctggtgg tgctagccgt ccccacggag gacctgccga accacggagt ccggctccag
     5461 gagaagaact cggccgtcgt gaacgcgagg ctgctgaagc cggccggcgg agacgaggaa
     5521 caatatggcc ggcggctcga tcgcgaggtg atgatgagca tcgggctcct gatgggcctc
     5581 gaaacctgcc cgaatcccca atgcgcgatg tggctctatt ccaacgacga ggaactcgac
     5641 atgaagggcc ggaactactg tcctccctgc ctggagcgga tccagaaact ggccgtggag
     5701 cgggggctga agatcgtggc cgattcgccc ttcgcgccga tcccggatga gcccgtgccg
     5761 gaagaggctg cctcggaacc ggccgcgccg gaacaggcgg ccccggccga ggccggcgag
     5821 ccggaaccga ttgcggaaac cccctgaccc gccgggccgc ccatgctgga tatccggacc
     5881 atccgcgagc gcgaggccga cgtgcgccgc gccctcgacc tgcgcggcgt cggcgtggac
     5941 cttgacgccg tgctggccct cgaccgcgac cgccgcagcg gcctggccga ggtcgaacag
     6001 ctcaagaacc agcgcaacgt gatctccaag gagatcggcg ccctcaagaa ggccggccag
     6061 gataccgccg cccgccaggc cgaggtgagg gccatgggcg atcgcatcgc ggaactggac
     6121 cggatcgtcc gcgaggcgga ggagaagctg aacaatgccc tgctgttcat gcccaacctg
     6181 ccgcaccccg ccgtcccgcc cgggcacgac gctgcgcaga accgcgtcgt gcgccagcac
     6241 ggcacgccgc gctcgttcga cttcgagccg aagccccatt gggaactggg cgagaaactc
     6301 ggcctgttcg acctcgagcg cggcgcgcgc atgagcggcg ccggtttccc gctcttcacc
     6361 gggcgcggcg cgcgcctcga gcgcggactg atccaattca tgctcgacct gcacaccacg
     6421 cagcacggct accgcgagat cgctccgcct ttcctctgca acgccgcgtc catgaccggc
     6481 accggccaac tcccgaagct cgccgaggac atgtaccacg tcgcgtcgga cgatctctac
     6541 ctgatcccga ctgcggaagt gcccgtgacg aacttctacc gcgaggagat catccgcgac
     6601 ccgctgccgg tcaagctcac ggcctactcg gcctgcttcc gccgcgaggc cggcgcggcc
     6661 gggcgcgaca cccgcgggct cattcgcgtg catcagttcg acaaggtcga gctggtcaag
     6721 ttcgtcgagc ccgagacgtc cgacgcggaa ctggagacgc tcctcgcgga cgccgaggac
     6781 gtgctccagc ggctcggcct ggtctaccgg atcctggagt tgtgcgcggg tgacttgagc
     6841 ttcgcgtcga gccataccta cgatatcgag ctgtgggcgc cggcgcagaa ggcttggctc
     6901 gaggtgtcgt cgtgcagcaa ctttggcgac ttccaggccc ggcgcgccgg catccgctac
     6961 cggaacaagg acggcaaggc ggcgttcgtg cacacgctca acggctcggg cgtggccctg
     7021 ccgcgcctcg tcgtggccct cctggagaac ggccagcgcg ccgacggctc gatcgtcctg
     7081 cccgaggccc tgcacccgta cctgggcggg ctgaccgttc tggattgacc gcggggccgg
     7141 tggggtcgag gattccgccg tgctccagat catccagcgc gcgatccgcc gtcagaacct
     7201 ggtgacgccc gggacgcgcc tgctcctcgc cgtctcgggc ggggcggatt ccacggccct
     7261 ggccgcggcc ctcgccgggt tgcgcgttcc cctgaatctt cgactgaccc tggcccacct
     7321 gaaccatggc atccgcggcg ccgaggccga tgatgacgcg cggttcgtgc gcgcgctggc
     7381 ccggcgtctg cgcgccggtt tcgtcctggg tagggcggat gtcccaggcc tgtcgaggaa
     7441 aaagggcata tccatcgaaa tggccgcccg cgaggcgcga tacgatttcc tggcgcgcgc
     7501 ggcccggcgg gccggcgcgg acgcgatcgc cacggcgcac accgccgacg accaggccga
     7561 gaccgtgctc ctgcggcttg cccggggaac cgggccgcag gggctggccg gcattccgta
     7621 ccgctcggaa cacggcggag tgccggtgat ccggccgatg ctggacgcga cccgcgcgca
     7681 ggtggaggcg tttctgaagc gccggggcct cgggtggaga gaggactcga ccaaccgcaa
     7741 cccggagcac ctccgcaacc gcgtgcggca cgaaatcatt ccgctgctcg aggcgcgcct
     7801 gaacccgtgc ctgcgcgagg ccctgtgccg cctcgcctcg ctcgcggggg aagagaaccg
     7861 ctggatggag caggaggcgg cgcggctcgc gttcgacggg ttcgtggcgc agttggccgg
     7921 cctgccggtg cccctggcgc ggcgagccct ccgggcgtgg ctaagtaaaa cgggtgttcc
     7981 gcccgaatcg ctcgattttg aggccatcga gcgcgtccgg gctctggcgg agggtcggcg
     8041 aggcggggga tcggcgccgc tgccaggggg gtggcgggtg acccgggagg ccggcgcgct
     8101 gcggattcag aaagctggtc gcgcggggcc gtcttcttat tcgacgcggt tgaaagttcc
     8161 cggcgtcagc tcgctggcga gcggctggcg agtgcgagtg gttcgcgggc gcggttatgc
     8221 cgcgccgagc gctgccggga acctggaggc ttgggtggat gccgggcggg tggggcggtc
     8281 gccactgatg gcccggtcgt ggcggcccgg cgaccggatc cgactcgttg gtctggcggg
     8341 atcgaagaag cttcaggatc ttttcgtcga ccttaaaatc ccccgggagc agcgcctgcg
     8401 tatcccggtt attgagtgca agggggagat cctttgggtt cccggcagcc gcgtatcgcg
     8461 ggattgggcc gtcagccatc ctcaagcgcc ttcatggcgt ttgtccgccc ggcttcctcc
     8521 cgccggctaa aaaaaacgtt ttttcctttg ccaaacggag tgcgccccgc cattattcgg
     8581 aaccgcggcc ggacatccaa tcaggaactg gccgcttctg cgaaaaggac gggaaacgaa
     8641 ggagggtagg gtcatgctgc tcggatttga ggatccttgg gtctgcaccg tgtacatctt
     8701 gtgcattgcg agcacgctgc tgtgcgtggt ctacgggttg gtcaattgga acaagggcga
     8761 ggagactgtt cagcccgagg acgtgcagtg ggccaaggaa gagaaggaag aagtcgagga
     8821 gaccctgtaa gtcgccggtt cccggcgccg ggtcaatcaa gggggatccc atggatcttt
     8881 tcagcatcat catcgtgctg gtgtatttgc tggccaccgg ctacctgggc taccagggct
     8941 accggcagac caagaccgcg gcggactacc tcgtcgcggg ccgcagcgcg catcccgtgg
     9001 tcatggcctt gtcgtacggg gccacgttca tcagcacctc ggccatcgtc ggcttcggcg
     9061 gcgtcgccgc cagcttcggc atgggcctgc tctggctgac gttcctgaac atctttgtgg
     9121 gcatcttcat cgccttcgtg gtgtttggca acccgacgcg gcggatgggg caccacctcg
     9181 acgcccatac gttcccggag ctcatgggca agcggtataa cagcaagttc cttcacgtgt
     9241 tctccgccct gatcattttc ttcttcatgc cgctctacgc gacggctgtc attatcggcg
     9301 gcgcggagtt catcgcgccg atcttccgcg tctcgtacga cacgggcctc tatctgttcg
     9361 cgctcatcgt ggccgcttac gtcatcgccg ggggcctgaa gggcgtgatg ctgacggacg
     9421 ccctgcaggg cgcgatcatg ctggtgggca tggtcatcct tttcatcacg acctattcga
     9481 tgctcggcgg catcgtgccg gcccacgaag cgctctcggc catgaaggac ctggtgccga
     9541 aggggctcca ggcgatcggc catcgcggct ggaccgccat gcccgaattc ggctggtccg
     9601 cggccggggc gcccatgccc gacgccctgc ggcataacct gtggtgggtc atggtcacga
     9661 cgatcgtcat gggcgtcggc atcggcgtgc tggcccagcc ccagttgatc gtgcgtttca
     9721 tgaccgtcaa gtccaagcag gcgctcaacc gcgcggtcat catggggggc atcttcatct
     9781 tcttcatgac cggcgtggcg ttcatcgtcg gggccctgtc caacgtgtac ttcgcccaga
     9841 gggagaagat cgagtgccgg atcgtggccg agaacgtgtt gatggatccc ggggcggacg
     9901 gcaaggccaa gctgacgctg gttcccgaga acgcggcgga cgaggtcaag gcgcgggcca
     9961 agccgttcgt cagttaccgg ctgtccggcg acgaggagag gcagcccctc tcctacgtga
    10021 tgcagacgcc gggcacggtg attacccgtg gcgtggacgg cgcgccggac atcctggagc
    10081 cgcacctggt ggcggttgcg cgcagcgtga cgctgggcac gcctttgggg ggcaacagcg
    10141 acacgatcat ccccacctac gtcaaggcgg cgctgccgca ctggtttgcg gtcgtcttcc
    10201 tgctgaccct gctttcggcg gccatgagca cgctgtccag ccagttccac accatcggca
    10261 cggccgtcgg acgcgatgtg ttcgaggagg tgacccacaa gcgccacacg gagaagtcga
    10321 tcctggtaac gcggttcggc atctgtatcg gcctgctcgc ggccgtcatc gcgggcaagg
    10381 tcatccgcgg gaacatcatt gccctggcca cggcgatctt catgggcctg tgcgccgcgt
    10441 cgttcctgcc ggcgtttgtc ggcggcctgt tctggcggcg catgaacaag gcggcggcgg
    10501 tgagttcgat ggtggtcggc ttcgtctcga gcacgttctg gctggccttc gtgaacggga
    10561 agaccgcggc cggcctgggc ctctgcaagt tcattttcgg caaggaccac atcgtcccgc
    10621 cgacctggag cgtgacgtgg acggtagtgg atccgctcat cgtggcgctg ccgctgtcca
    10681 tcctcgcggc cgtcgtcgtg gcgctgatca cgaagccgat ggacaagacc tacgtggact
    10741 ggtgctacgg cggaccgccg ccgaaggccg agtagatccc gcgacaggcg tgacaacaat
    10801 ttggggaccg ggcgcgtggc cggccccctg aaaagaaaag gagagcacga atgaagacat
    10861 cggtacgcat gttgcagtgc gccctgctgg ccctggccgt cctgtggacg gcggagcccg
    10921 cgtgggcggc gaagaagaag gccaagaagg ccgacgaacc cgcgaagacc gaggcggtcg
    10981 ccccggcgga acaggcggcc ccgcccgcgt tccgctacgg cgccgacttg cgcctgcgcg
    11041 aggaatactt cgacaacatc ccgatcatcg ccgacccgcc gggcgtcacc cgcggggggg
    11101 agaacaacta cttccgcgtc cggccccgga tctgggcgga atacgacgcg atggagaacg
    11161 tgactttgcg ggcccggttg gtcaacgagt tccggatcta tgacaagccg gacatggatg
    11221 cggcgcccca gcgctcgaac tatgagttcc ccgacgagta cgtgttcgac cacttgtacc
    11281 tcgacgtgcg caacctgctc ggggacaagc tcgacctgcg cgtcggccgc caggagctga
    11341 tctacggcac cggcaaggtc atcctcgagg gcacgcccaa ggacggctcc cgcacgatct
    11401 acttcaacgc cgtcaaggcg gtgtggaaag ccatgcccaa gaccacgatc gacgtcttcg
    11461 gcatctacaa tccctccatc gatgacctgg ccatcaacga cgccgaccgc gacctgacgg
    11521 gcgtcaccag cgccaacgac gacatcaccg agagcggcgc cggcctctac ctgaagaaca
    11581 agtccgtcga gaaatgcccc tatgagctct attacgtcta caagaacgag agcgactggg
    11641 acaaggtcgc cggcaccaac gctcccgtgg ccattccgga gtcggacatc cacaccgtcg
    11701 gcttccgcgt catgccccag cttgccaagg acctgaaggg caacctcgag ttcgccttcc
    11761 aggcgggcga gcagggagac aaggacctcg agggctggat gctcgacacc gcgttgagct
    11821 acacgatccc gatggccgcc gacacgctgg cccccgtggc cgatgtcggt ttctacgccc
    11881 tctccggcga cgacccggac acggcggaca agaacgagag ctggaatccc ctgtgggccc
    11941 gctacccgca gtacagcgag ctctatatct actcgtggga cgcggacgcc gccggcaagt
    12001 ggcagaacgt gatgatgccg tacgtgcaga tgtcgctcaa gccctgcaag aagagcaagc
    12061 tcacggccat ggccggctac ctgctcgcgc ccgaggacaa cggcccgggc agcggggatg
    12121 agcgcggcat gctgctcgtg ctcaaggacg agatcacgat cgccgagaag ctgctgtcct
    12181 ccaaggacaa gctgaccggc catctctggg ccgaaatcct ggagcccggc aactactaca
    12241 acgtcgacga caccgcctac ttcctgcgct gggagctgat gtacgcgttc tgatcgcttg
    12301 aatggagcct gacgcgacgg gccgcgggga tcctccccgc ggcccgttgt ttttcaggcg
    12361 caccgcgcct tacggcgcgg ctacgaacaa accgtttgcc gcgcggccgt caggccgccg
    12421 ggtcggcgcg cccaaaaaaa gattgcctgc cgcggggata atcgccagta ataaccacac
    12481 acggagaaga cgccatgatc ttcaacaaga ccttcgaatg catggatcgc gcggcgctgc
    12541 ggaacctcca gggcgagcgc ctgcgcgatg ccgtgaaacg ggcctacgag catgtcccgt
    12601 tctaccggag ccggctggat gcccacggcg tgaagccgtc cgacatccac ggcatcgagg
    12661 acatcgtcaa gctgcccttc acgacgaagg aggatttccg ggacacgtac cccttcggcc
    12721 ttttcgccgt gccgctccgc gagatcgtcc gcgtccacgc ctcgtccggg accaccggca
    12781 agccgaccgt ggtcggctac acgaaggaag acatcgacac gtgggccgaa accatcgccc
    12841 gcaccctggg ttgcggcggg ggcacggcgg acgacatcct ccaggtggcc tacggttacg
    12901 gcctgttcac cggcggcctg ggcctgcact acggcggcga gaagctcggc gccatggtca
    12961 tcccgatgtc cggcggcaac acgaagaagc agatcatgct catgcaggat ttcggcacca
    13021 cgctgctggc ctgcacgccc tcctactcgc tgcagatcgc ggaggaggcg gccgcggacg
    13081 acatcgacct caaggcgctc aagctgcgcg tagggtactt cggcgcggag ccctggaccg
    13141 aggccatgcg ggcgaagatc gaggagcggc tgcccgtcaa ggccatcgac atctacggct
    13201 tgagcgaggt catgggcccc ggcgtggcca gtgaatgcct ggagcaggac gggctgcacg
    13261 tgttcgagga ccactacttc ccggagatca tcaacccgac gaccggcgag cccgtcgccg
    13321 ccggccagaa gggcgagctg gtctacacct gcctgacgaa gcgctccacg ccgctgctcc
    13381 gctaccggac gcgcgacatc tccagcctgg acgagaagcc ctgtccgtgc gggcgcacga
    13441 gccggcgcat ggcccggatc acgggccgca cggacgacat gttgatcatc cgcggcgtca
    13501 acgtgttccc gtcgcagatc gagaccgtgc tgatgaaggt cgagggcatt gagccccact
    13561 acgtcatcgt tgtggacaag acgggcgcgc tggacgagtt ggagatcaag gtggaggtgt
    13621 ccgagcagct gttctccgac gagatccgca agctcgagga attgcgggcc cggattttcg
    13681 aggagatgcg cagcgtgttg agcttgagcg cgaaaatcac gctcgtggag ccgaagacca
    13741 tcgagcggac gctgggcaag gcgaagcggg tgatcgacaa gcgggaagtg aaataaccgc
    13801 gccgcgaggc ggcgggagga gaggccacga tgaaagtcag gcaactgtcc atcttcatgg
    13861 aaaaccgggc cgggcggctg gccgagatca tgcaatcgct gggcgacgcc gggatcaaca
    13921 tccgggcgct gtcgctggcg gacacgtccg acttcggcat cctgcgcctg atcgtcagcc
    13981 aggtggacaa ggcgctcgaa gtgctgcgga aggccgggca caccgtctcc ctgacggacg
    14041 tggtggccgt cgaggtgccg gaccacccgg gcgggctggc cagcgtgctg acgccgctgc
    14101 gcgaggccgg ggtgaacgtg gaatacatgt acgccttcgt ggagaaggcg acggacaagg
    14161 cggtggtcat tttccggttc gaggaaaccg aggccgccct gaaggtgctg gccggggccc
    14221 gcatctccgt cctgcccgcg gagaccgtct acaagctatg aagtgaatcg tgaatggtga
    14281 gtcgtgaata gtgacggcct gaaccccgag tcctgaaccg ctctcttcct ccatgatacc
    14341 ttccgccatt tgggatcctg aattcgaatg catgcacgtc gacgccctgc gcgaactcca
    14401 gctggagcgt ctgcgcaagg tcgtccggcg cgcttggggc cacgtgccgt actaccggca
    14461 gaagatgcag gtcgtcggcg tgacgccgtc ggacatcaaa acgctcgagg acatccggaa
    14521 gctgcccttc accacgaagg acgacctgcg gctctgctat ccctacgagg ccttcgccgt
    14581 gccgctggag aaggtcgtgc gcatccacgc ctcgtccggc acgacgggca agtccaccgt
    14641 ggtcggttac acgcgcaagg acatggaggt ctggaccaac gtcgtggcgc gcttcctcgt
    14701 cgccggcggc ctgacgtcga aggacatcgt gcacatcgcc ttctcgtacg gcctgttcac
    14761 cggcggcttc ggcctgcact acggcgcgga gcgggtgggg gcctcggtga tccccgtctc
    14821 ggccggccgc agcgagcggc agatccagat catgagggac ttcggctcca cggcgctggt
    14881 ctgcacgccg tcctacgccg tctatctcgg cgagctcgtg cagggcatgg gcctgaagcc
    14941 cggagacctg aagctgcgcg tcgggttctt cggcgcggag gcgtggtcga accgggtgcg
    15001 cgaggagatc caggccaagc tgggcctctt cgccacggac aactacggct tgagcgagat
    15061 catcggcccc ggcgtcgccg gcgagtgcgt ccaccagaac ggcatgcaca tctcggagga
    15121 ccatttctac ccggagcgca tcgatcccga cacctgcgag cccgtgggcc ccgaccggcg
    15181 cggcgagctg gtgctcacga cgctgaccaa ggaggcgatc cccctgatcc gctaccggac
    15241 gcgcgacatc tgcatgctga tgcccgggac ctgcccgtgc gggcggaccg gcgcgcgcat
    15301 gtccaagatc acgggtcgct cggacgacat gctcatcatc cgcggcgcga acatctaccc
    15361 ctcgcaggtc gaggaggtgc tgctcgatgt caagggcacg ctgccgcact accagctcgt
    15421 ggtctcccgc gagggcgcgt tggaccgtct ggaggtccgg gtcgaggtct ccgaggagtt
    15481 cttcgcggac gagatgaaga agctcaacga gctcgaccgc cagatcgagc acaagctgga
    15541 gaacgtcctg ggcatccggg ccagcgtcaa gctggtcgag ccccgcacca tcgaacgctc
    15601 cgagggcaag gccaagcgcg tcctggacct gcggccgaag gagtaggccg cttccttgcc
    15661 ccagagaccg tcaggccggg caggggcggg tttaccccgg ccatccgccc gtccggcgcg
    15721 cgcccccggc atgtcaattt gaaggtggaa acctccccgc tttttctgta taagaaacgc
    15781 tttaatcggc cggggacctg cgcgtgacca ttcgctcgat catactgggc ctgctggggg
    15841 tggcgttcgt ctgctcctac acgtatttca acgaccacgt catgcgccag acgttcttcg
    15901 tgggcaacaa catgcccatc tccgtctacg ggctgctcgt cgtcttcctg ctgttcatct
    15961 acccgctgtt ccggaagatc agccggcgcc tggcgttgag ccggcgcgag atcgtcgtga
    16021 tcctggtcat gacgctggct tcctgcgcca tccccggctc gaacctgctg cgcctcttca
    16081 cccagaccct tatcctgccg caccggttcg agcgcacgga gccgggctgg gagaagcaga
    16141 aggtcatgga cctgaccccg cccgggatgc tggtggacgt cagcggggac gaggagcgcg
    16201 tgttgaacgg cttcgtccgc ggcttgagca tcgccaacga gtccatcccc gtccgggcgg
    16261 tgccgtggaa ggcgtgggcc cggccggtcg ccttctggct gccgatcatt ctctcgctct
    16321 ggctggcgat ggtcgcgctg tcggtggtgg tccaccggca atgggtcgcg cacgagcacc
    16381 tgccgtaccc gatcgtcacc ttcacggagt cgctgctgcc cgaggaggac ggccggcgca
    16441 gcccgatctt cgggaacaag ttgttctggc tcggcgcgct gctgcccttc gccatccaca
    16501 cctacaatta cctcgtcgtc tggttcccgg accagacgct gggcaagatc ccggtcggcg
    16561 tggatttccg ctcgctggtc gagctgttcc cgaccttcaa gaaaggcggc ggcgaggcgc
    16621 tgctcggcta ctggcagctc tacttctccg tgatggccat cgcgtacttc ctgcccaagg
    16681 acgtttccct ctcgctgggc atcggcccct tcttctggac gctggtggcg ggcgtgctga
    16741 cgagctacgg catcgcctcg acgggctggc agggcagctg gccgttcggc atccagcgcg
    16801 agggcatggt ggtcatgggc tcctacttcg ccatgctgct ggtgatcctc tacacgggcc
    16861 gccagtacta ccggaccgtc ttcgcgcggg cgctcggcct gcggacgaag gaccccgtcg
    16921 agcccgcgct ggcgtggggc gcgcgcgtgt tcctggccgc ggtggccctc ttcgtggcct
    16981 ataccgcgat cgtcgcgcgg ctcgactggc cgctggccct gctgttcggg ctcggcatca
    17041 tcggcgtcta cctcgtcatg agccgcatga tcgcggagac cggcctgttc ttcatcgtca
    17101 cctacggcac gccggcgacg atcctctggg cgctgctggg cgcccgggcg ctcgggccgc
    17161 agatgctgct gatcatgttc atgttgagcg tgatcctctt ctacgacacc cgcgaggcgc
    17221 tgatgcccta catcgtcaac agcctcaagt tgagcgagga ctgcaaggtc cggatcggcc
    17281 gcgccgccgg gctcatggtc ctcgccgtcg tcgtcggcct ggccgtcggc atcccggtga
    17341 cgctctactt ccagtacaac cgtggcctcg acgtggccgc gtggcagaac cagcagccca
    17401 aggtccctta caacgaggtc atcggcgtga tgcagcgcct cgaggcgcag ggccagctgg
    17461 aggaggccgg cacgctgacg ggcctgaagc ggttcgcctg ggcgggcccg aacaagctgg
    17521 ccctgaccac gttcggcgcc ggcgcggcgc tcgtgctggt gttctccgcc ctccgcctgc
    17581 gcttcgccgg gtggccgctc catccggtga tgttcctcgt ctggtccacc tactcgggcc
    17641 agtgtttcgc ccagtccttc atcgtgggct ggctgatcaa gtcgtccgtg atgaaatacg
    17701 gcggggcctc gatttaccag aagttgaaac cggtcatgtt cggcgtcatc gccggcgaga
    17761 tgctcggcgg catggtgccc atggtggtca gcctggtcta ctacctggtc accggcgacc
    17821 agccgaaaca gtttctgatc atgccggggt gaaatgaaca gaaggcgcaa aggacgcaaa
    17881 gacgaagcgc gagcaagagc acgagcagga gcacgatgct gaatcctgaa ccttattccc
    17941 tttttcatga agcacattga tgtccagccg cagccggtct tgagcttcgg gcgcaccttc
    18001 gcgatcgcgc tgcgcggggt gcagtaccgg ctgttccgcg ccctggtgac cgtcgcggtc
    18061 atcgccgtgg cgatggcctt cctgatgaac atcttgagcg aaagcctgtt caagcgcgtg
    18121 gtggccgtcg ccgtccgcga cgagatccgc gtcagccggc aggcggacca ctggatcgcc
    18181 cggctgtccg tgccgcagac cgcggaggag gtcatcgacg acctcgcggc cgccgagccg
    18241 ggcaccccgg cctcccgcga actggcccgc ttcagcgggc gggacccggc cgcgatcgag
    18301 caggcgggca agcaggccgc gcaggcccgg gagtacttcc gcttcttcga cgacatcaac
    18361 tacggccgcc gccgcgtgct gatcggcaac gccgccggca tcgggatctt cgagcggctg
    18421 caggatccgg aggcccaggc ccgcttcttc gagagcctgg gccagatgcg ggccgtgcgt
    18481 ttcatcgcga cgcccgacgg cttccgggcc ttcctcgctt cctggcctgc cctgcgcgag
    18541 gtcgtggccg ccgtgcgcga ggggcagcga cagggcatcg gacaactgca cgcgaagctg
    18601 gacgggcgga ctgtcatggc cgcgctggcg gacgcggagg gggcgttcgg ggaggctgtc
    18661 cggcaggccg gattcgtgct cgagccggaa gaagcgactt cgctggcgaa gcagattcgc
    18721 ctgtccgagc accggcactt catcgaggac agcctcaacg tccccgaggt ccgcaaggcc
    18781 gtcgccgccc gcaaggacgt cctgcccggc gacgtaagcc tggacatgat ctggggcctg
    18841 ctcggccatc ccgacacggc ggcgtggtac cgggacctgc tggcgcagca cacctcgccg
    18901 ccgcccggcc tggacggcga ccggctgcac gaggtggccc gcgcccgcgg gcgctcgaag
    18961 ctgatggtcc gcgcggagct gatcaccatg ggcaccggcg gcgggttcat gaacatcggg
    19021 ccgcgcatga cctggctggc cttcgtctcg atgctggtct gcaccgtggg catcgccaac
    19081 gccatgctga tgagcgtcac cgagcgcttc cgcgagatcg ccacgctcaa gtgcctgggc
    19141 gccctcgacg ggttcatcat gacggtcttc ctgatcgagg ccgcgatcct cgggctggtc
    19201 ggcggcgtcg ccggcacgct gatcgggctg gtgctgggcc tcgggcgcat gctggcggtc
    19261 ttccaatccc tgttgctgga actgttcccc gggggcctgc tgctccaggc cgccggcatc
    19321 tccatggtca cgggcatcgc gctggcggcc gtcgcggcgg cctacccgtc gctgcgcgcc
    19381 gcgcgcctgg ccccgatgga ggcgatgagg atcgagtaag aaacggatat cggcagggcg
    19441 cgttggctca acgcgccggg gtgacggcgg cttgagccaa gccgccctac cggatggcga
    19501 agaagaaatg gcgaagaaag aacaagcgcc ggattcggcc gaaaagcggg tgatcatccg
    19561 cacgatcggc atgaagaagc tctatcgcaa gggcgggcag gagaccgcgg ccctgcgcgg
    19621 ggtggacgtg gagatctaca ccggcgagtt catctcggtc atgggcccgt ccggctcggg
    19681 caagagcacg ttcttcaaca tgatcggcgc gctggacagc ccgagcgagg gccgcgtgtt
    19741 catcgacgag gtggacgtgg cgcagttgac cgccgaggaa ctggcgttcc tgcgctgccg
    19801 caagatcggc tacatcttcc agagctacaa cctgatcaag tacatgaccg cgctcgagaa
    19861 cgtgaccacc ccgatggcgt tcggcggcgt gccggacgac gatgcccgca agcgcggcat
    19921 ggagatcctc gggctcgtcg gcctgcgcga gcggtggttc caccggccca tcgagatgtc
    19981 cggcggccag cagcagcgcg tcgccatcgc gcgggccctg gccaaccagc ccgcgatcct
    20041 gctctgcgac gagccgacgg ccaacctgga ccaccacacg ggccaggaga tcctggatat
    20101 tttgcagaag ctgaacaagg aatgcggcgt gacgatcatc tgcgcgacgc acgaccaccg
    20161 gatgatgaac gtctgcgacc ggctgatgtg gatccgcgac ggcagcatcg agcgcattgc
    20221 gcgccgcgac gaggtccacg tggaactggc gggaatcgac taggcatgag ggccggcgcg
    20281 tcattttatt ccgttgccgt tttggagcgc gcgggcaagc ggagcgcgac cgcgcttggc
    20341 tggctggccg ccttgctgct ggcgaccgct gcccgcgcga tatccccgga gttcctcgcc
    20401 gactgccagg cgctgacgcg gcacccgcac cggctggcgg gctcggagga gtacgccgac
    20461 gccgcgcggc acgtggaaca gcgcctgcgc gaggcgggcg tgacgaacgt gctggtccag
    20521 acgttcccga cggtccagaa cgtcgtccgc cggtgcgagg cggtcgcggc ggacgggcgg
    20581 acgttcccgc tgctgcccat gcgcccggac ggcatcgtgc cgcccgtgac gccgccggcg
    20641 ggcatcacgg ggaggatcct gcgcctcggc cgcggggagc ccggggattt cgaccgtgag
    20701 gacccgcggg gccgcatcgt ggtgatgaac tacaacgcgg aggaccgctg gctcaaggcg
    20761 ttccggctcg gcgcgaaggc cgttattttc gtgaaggagg aaacggccga cgccttccat
    20821 ttccattacg tgcgggccaa cgcgaacctg ccgcgctttt actacgacgg gccggcatcc
    20881 gacctcgcgg acgggcagga gctcacgatc cacagcgagg tggcctggca gcgggcaacc
    20941 ggccgcaacg tgttcgggtt catcgagggc aaggtgccgc tgttcgacct gggcaaggag
    21001 gaggtgctcc tgatcgcggc gccgttggac accttcggcg aggtgccgca gcggacgccg
    21061 ggcgcgcagc cggcggccaa cgcggcggcc gtcctgaagc tcgccgggat gctggccaag
    21121 gccccgccgc gccggcacgt gctgctggcc ttcttcgacg ggcacggccg caaccacgcg
    21181 ggcgtcgccg ccttctacca ggccctggaa accaaggccc gcgacgcgac gatcgaggag
    21241 cggatggccg cgtggaaggg cgagtcggag ttcctggccg agaccgagaa actggccgcg
    21301 cagcccaacc cgctggccgg ccacgcgggg gacgccggcc gcgaactgct gctgcggatg
    21361 cgcaacaagg cggcggaccg cgcctacgag atccgcgggc ggctgatgga cctgcgcgag
    21421 gcgcgttcga aggctgggcg cgattcgccg gcctacgccg acctgcaggc ccgcgaggac
    21481 gaactcaacg gggtgtacaa tcgctggaac gaactgcgcc ggaagctcgg gaagggcggc
    21541 gccgacgccg ccgggagcga ggaattcgac gccgccctgg ccggcgtgcg tcgggacatc
    21601 gcccttcgcc gcgcggagct ggtcgaggag gagcagatgc tccgctcctc ggccgcgatc
    21661 aaggccctgc tgggcgagcg ctggatcgcc ctgcacgcga cgatgatgct gggcgaccgg
    21721 acgccgacct ggggcgtggt cgtcggcggc aactcggaga tccgctccac gcaggatctt
    21781 ccgggcctgt acggccgggt ccagcggagc ttcctgcagg cccatcgctc gctcgcgcgc
    21841 cgcgggaacg gcgcgcccca cttcgagccc gtctcggtgg acggctcgct gcgcgacgtg
    21901 cggctgctct gggcggcgcc gatgctgatc cacggcggcg agctggccgg ccggcacggc
    21961 gtgtataacg tggtcctcgg gacgctgcac gagaccctgc cgcggctcgg cacgccggac
    22021 gacacgctgg accggctgga cgcggaccgg atcgagcaga acacggcgga ggtcgcgctg
    22081 ctgctcgcgg cggtgggcag cctcgagggc gcgagcggcg acgtcgaggc cgtggccgac
    22141 cagcgcggcc tctccctgcg ccgggcgatc atggcggaca acgattacgt ggtcagcgag
    22201 ttctccggcg ggcggacgca gggggcgctg gtgatgggcc ggcaccgcgg cagctcgatc
    22261 cccaaccggc ccgtgcccgg cgcggtggtc caggtctcgg tcgtggaccc ctggaacagc
    22321 gtcttctggc ggcacagcaa ggcgccgggt ttcgatccct tcacggtggt catgaccgac
    22381 cagaacggcg cctactcgct gaacgccctg ccgacgcagg acccggcgcc gatcgcgttc
    22441 gccgcggcgt tcgacgggcg cgggctcgtg aaccaggtga ccgacatgac ctcgcgcaag
    22501 acggtccggc agcgcctgaa catgttccgg tgcgcgggcg ggctgctggt cctgccgccg
    22561 atgcactgga cgcagaaggc gaccttcatg agcgcgcgcg ccaacgcgcc gctggcgcag
    22621 ccgaccgcga cggattcgta cggcgagacc acggacgggg tcgcgtactg gtacggcgag
    22681 gagaagctgc ccggcatcaa ggcgttcggc aaggacgccc tcgtggcgct gaacaacgtc
    22741 cccgtcgagg ggcagaagcc cgatccctac gggcgcggct acccgaggag cgcggagtgg
    22801 acgaccctgc gcaacacgga gggctcggcc tgggacctct ggcggctcaa cgaagcgcgc
    22861 ctgtccattc tgcgcagccg cgacattctc aacagcgcgc tcgaggaggc ccacgggcgc
    22921 gtgcgcgacc tgctcgacga ggccgcggcc ggcccgtcgc tggtccgcaa cgaggccctc
    22981 tgcgcgtcgg ccttcgaggc ggctcggccg gtctatacgc aggtgcgcga gagcatggac
    23041 gacctggtca aggcggtgct cgtgctcctg gcgctctgca tcccgttcgc gttcgcgctg
    23101 gagcgcctgc tgatcggggc ctcgctgatc tacaagcagg tggcctggtt cgtcggtttc
    23161 ttcgtggcga cgttcctgct gctctacgtc tcgcacccgg ccttcgcgat cgcccagacg
    23221 ccgatcatca tcttcctggg cttcgccgtc gtggtgctgt ccgtcctggt gatcgtcatc
    23281 atcatgcaga agttccaggt cgagctgaag cggctgcagg ggatgacctc gaccgtgcac
    23341 gcggcggaca tctcgcgctt cagcaccgtc atggccgcca tggcgatggg catctcgacg
    23401 atgcggcgcc ggccgctgcg gacgacgctg acggcggtga cgatcatcct gctgaccttc
    23461 acgatcctcg gcttcgcgtc gttcgacacg cagcgcggga tcgtgaagct gttcgtcgcg
    23521 cccgcgccgc cgtacacggg cgtgatgctc aacaacccga cgtggggctc gttcaacgcc
    23581 gagttcgtgg acctcgtgcg cagccgctgg agccgcgagg tgaacctgtg cctgcgctac
    23641 tggatatgcc cggagttcaa ggacgacccg gacttcgtca tcgcgctgga ggacggctcg
    23701 aagccggtct cgctcaaggc cgtcctgggg atcccggcgg aggaaatcgc ggcgcggccc
    23761 gacctgaaac agcttttccg gctgaacgac ccggcggata tcgaccgcat ggtgctgatg
    23821 accgaggccg tggcgacgct gctgaaggtc aagcccggcg accgggtctg cctgaagggc
    23881 aagatcctgg aggtcggccc cctgttgagc gcggccgatc tttccgccgc gcgcgagatg
    23941 gagggcagca gcattctgcc cgtggacttc gccacgatga agctcgcgag cgcggggacg
    24001 gccgaggtga ccgagaacgt cctcgacgag caggccaagt ggacgagcct gccggtggat
    24061 tccgtggtca tcacctcctc ggaaaactcg cggctgttcg gcggcaagcc gcacctggtc
    24121 cacttttaca cgaaggacgc gcaggaggcc gcggggatcg ccgaggagct ggcccgcatg
    24181 ctcgaccggc acccggtgat ggcgacgcgc atggacggcg tctatcggca cgtccagggc
    24241 acggtcgtcg cggccagcgg ggtgggggac ctgttcttcc cgatcctgct cggcggcctg
    24301 gtgatcttcg gcaccatgct cggctccgtc gcggaccgcg agaaggagat ctacaccttc
    24361 agcgccctcg gcctggcccc gccgcacgtc gcgagcctgt tcttcgccga ggccatggtc
    24421 tactccgtca tcggcgggct cggcggctac ctgatcgcgc agggcacgct caaggtcctg
    24481 accgtcgccg ccaactacgg cctggtccgc gtgccggaga tgaactactc ctccacaaac
    24541 gcgatcgtca cgatcctcat cgtcatggcc accgtgctgg tctccgccat ctacccggcg
    24601 atcaaggcct cgaagagcgc gaatccgggc ctgatgcggc tgtggcgcct gccgccgccc
    24661 gagggcgaca tcttcaacat cgttttcccc ttcaccgtct ccgcctacga cctgaccggc
    24721 gtggtgagct tcctgaagga gcacttcgac aacttcggcg acgtcgggct gggcagcttc
    24781 atggcgcgcg ggacggcggt gcgctctctc gggggcggcg aactgggcct gacgtcgcag
    24841 ctcgcgctgg cgccgttcga cctcggggtg acccagctct tcgaactgcg cagcgccccc
    24901 agcgaaatca agggcatcga cgaggtcaac atacggctca cccgccgatc gggccagccg
    24961 aaggactggg agcggctcaa ccgcgtgctg ctcgacgacc tgcggaccca gttcctgctc
    25021 tggcgctcgc tgccgcagga aaccatggaa gagtaccgcc ggcggactct ggtggcgctg
    25081 aacagggagg gcggcgggcc tcctccgccg gttagccaag cgaaggaaac gatctgacca
    25141 tggcccagcg catcgacaag gaactcgagg aattcaggca ggtgatggag gttccctcgt
    25201 ccttcgagga gggcttcaac tggaccagcc tgctgggcgc gatcttcgtg gcgctgctga
    25261 tggtgcccgg ggccatctac atgggcctgc tcgccggcgt ggagaacatc gggtcggcct
    25321 cccagtgggt gaccgtcatc ctcttcatcg aggtcgccaa gcgcgcgcag aagacgctca
    25381 agcgcgccga gatattcgtc ctgttcttca tggcggccgc ggcgctcggc atgccgttct
    25441 ccgggctgat ctggaaccag ttcttcgcgt gcagcgacgc cgccatcgcc gcgggcatcg
    25501 ccgagcacct gccgcgctgg ttcgcgccgc cggcggggtc ggagtcgtac gccctgcgga
    25561 ccttcatgca caaggactgg ctgccggtga tcggcatggt gatcttcggc atgttcttcg
    25621 gccagctctc caacatggtc ctcggctacg gcctcttccg cctcgccagc gacatcgagc
    25681 gcctgccgtt cccgatggcg ccgatcggcg cgcagggcgt gatggcgctg gccgaagact
    25741 ccgacgagaa aggcgcggag gaaggcgagg ggcgctggcg gtggcgcgtc ttttccatcg
    25801 gcggcgcgct gggcctggtc ttcggcacgc tctacctgct gctcccgacg ctcacgggcg
    25861 cgctgacggg caacccgatc cagctcttcc ccatcccgtt cagcgacttc acgccgcaga
    25921 cgggcgagta cctgcccgcc gtcgcgacgg gcctgtcctg ggacttcggc aacatcatct
    25981 tcggcatggt cctgccgttc ttcgccatgg tcggcagctt cgtcggcctg atcgcgacgg
    26041 tcgtcctcaa cccgctgctg cacgccgtcg gcgtgctgcg caactgggtg ccgggcgact
    26101 cgaccatcgg gacgctctac aagaacaaca tcgatttctt cttcagcttc aatatcggcc
    26161 tggccctcgc gatcgcgctg gcgggtttct accaggtcat ccgcagcttc cagggccgcc
    26221 ggaagaccgc cggcggcgag ccggtccgcc tggcaccggc gggacgcggc gacatccggc
    26281 cgttcatcgt cgtcgtggtg tacttcctcg tgacgatcac gtatatcctc gtgtccggct
    26341 ggatggtgga ctggcaccgc gggatcatga tcgtgctgct gttcctcggc ttcgtgtaca
    26401 cccccgtgat cagctacgtc acggcgcgcc tggagggcat ggtggggcag gtggtcgagg
    26461 tgccctacat ccgcgaggcg gcgctgatct tgagcggcta ccgcggcgtg gccgtgtggt
    26521 tcctgccgat cccgatggcc aactacgggc agatggcgat tttctacaag cagtgcgagt
    26581 tgacgggcac gcgcttcccg agcatctgga agacccaggt catcctgttc ccgatcatcc
    26641 tgttgagctc catcttcttc atgaacttca tctggagcct caacgaggtg ccgtccgccg
    26701 tctatcccta cgcggagatg atgtggaagc tgaacgccga gaacgcctgc atcatgttct
    26761 cctccacgct cggcgagtac tcgatcttcg aggaggcgtt caacgtcctc tacatcgcca
    26821 tcggcgcggt gttcggcgtg gcgctgttcg gggccatgaa cacgctgggc gcgcccatca
    26881 tgctgaccta cggggtggtg cgcgggctcg gcggcgtgat gccgcactgg gtgatccccc
    26941 agttcatcgg cgcgctgatc gggcggtact atttccagaa gcggctgggc ctgcgctggc
    27001 ggcagtacat cccggtggtc agcgccggct tcgcgtgcgg catggggctg atcaccaccg
    27061 tgggcgtcgg gatcacgttc ttgagcaagg cgagcatacc gttgccgttc taggcggaga
    27121 agcgcatggc ggaagtgctg atagagatac gcggcgtgaa caaggtcttc gacctcggca
    27181 agatcaaggt ccacgccctg cgggacatgg acctggagat cttccggggc gagtacctct
    27241 ccatcatggg cccgtccggc tcgggcaaga gcacgctgtt caacatgatc ggcgcgctgg
    27301 accggccgac gtccgggacg gtcacgatcg ccggcgtgga cctggccacg ttgagcagcc
    27361 gcgagctggc cttcttccgg ggcaagcaca tcgggtacgt cttccagacg ttcaatctcc
    27421 tgcccgccta cagcgccgtg gacaacgtcg ccatgccgct cgtgttcaac ggggcgggcg
    27481 aggagcgggc gcaggcgcgc gcgaaggaaa ttctcgaacg cgtcgggttg ggccaccggc
    27541 taacgcaccg gccggacgag ctttccggcg gccagcagca gcgcgtcgcc atcgcccgcg
    27601 cgctggccaa cgaaccggcc atcgtcctcg ccgacgagcc cacgggcaac ctcgacctgc
    27661 acacgggcga ggagatcatc cagctgttga gcgaattgag ccgctccatg ggcgtcaccg
    27721 tcatttccgc cacgcacgac cacaagatgc tcgccacctc cgaccggatt ctctggatca
    27781 aggacggccg ccaggaccgc ctcgaacgtc gcgaggattt gaacatccgc gtcggaaccg
    27841 tgaagtaaaa aaaggggggg cgatgaagat gtggaaactg ctcacggggc atccatggat
    27901 atggacttcg ctgttcattc tcttcgctgt cgtgcatgtt tccctggcgc tggccattgt
    27961 ttcggacggc aagcggcttg gggcgagagg gagcggcaca ttcctggtgg ggccttggat
    28021 atggggcatg gccacgctga tgggtggggt gtatgccgcg gggatttact ggatcattca
    28081 ctactcgcgc ctaaagccgg ccagccgggg aatggcccaa catggtcagt gcggaccggt
    28141 ggattgatcc ccttctgcga acacgatgct tcgtgtgaag ccgtcggctt taaaaaagca
    28201 aagagcaaac atgagtgacc ttcgaaaact gttttgggcg gcggccgttc tattgtgcgg
    28261 agccctgggg ccggcggctt ccgaagaaat ccggtcttcg gttgtggtgg tggtcagaaa
    28321 cgatcaggtc tgggattccg agggcaactt ggatccggat gaaataggcc ggatgatgga
    28381 aatgggcttg caggctcttg ccggggcctc ttccgggcgg gccgcctttg agcgactctt
    28441 caagcccggg gagcggatcg ccgtcaaggt caatgcgatc accaagaatt ggatgtcctc
    28501 ctccaagccc gctctcgtgc agcaaaccgt tgatctgctt cagcaagtcg gcatttcccc
    28561 ggaccacata accatcttcg accggatgcc ccgcccaggg cgccatgagt tgggcccggc
    28621 cggttttgcc ctgaatacca acgcggggag cgtgcaagtg gtcgagggcg gaatgttggg
    28681 gccggaacgg cagcaaggct ccttgaaaac gagattctac gccccggtcg aggaatgcga
    28741 cgcgcttatc agcatacccg tgctcaagga ccatcacatc atggggatga cctttgctct
    28801 gaagaaccat ttcgggtcga tccataatcc gtggtcttgt catcaaaacg ccggagtccc
    28861 gggcatcgcg gaactgaacg gcctggagcc gatccagaag aagcaacgcc tgatcatcgg
    28921 cgatgccctg gcgggccagt acaacaaggg tccgcacgac gcgccgaagt atcactggaa
    28981 ggagaatgcg cttatcttcg ggttcgaccc ggtggccgtg gactttgtcg ctttcgaaat
    29041 cctggaggcc aaaagaaggc aggagggatt ggcctggccc gagtcaatgc gctacggcgg
    29101 gaaagcgggc gcgtacatcc tcgattgcag ccagcgcgga gtgggccgta agccggatcg
    29161 agtgattcaa ttgcgctgtt cgtccgatac gggtccataa gttggctgct ccccgcatgg
    29221 gtcgggcggg ttgccgtgtc gtccttacgg cggcaccggg gcttccatca gttccagcaa
    29281 cgcgtccgaa gggacgctgt atttccagat catgctggcc cacacgttgg tgctgtcatg
    29341 gccatgatcc atgacgggcg agacggagca gacgatcccc gccaccgcgc cgtgctggtc
    29401 gaagatgggc ccgccgctgg agcccggcgc gtagtctgcc gagatggcca gcgtgcgcaa
    29461 gggctccttc gggttcagcc gaccgctcaa gaacttgccg acgacggcgc cctgggtgaa
    29521 ggcgtagagg ttggggttgg cgtggtggat ggcatagacg ggggcgccca cggagatgtc
    29581 ctcggcgacg gggagcggcc gcaggccctg cgcgtccgtc cgcaggatca cgagatcgtt
    29641 gcgcggatcg gaggcggcca cttccttgac agggaagagc cggccatccc gcgtcaggat
    29701 gcccatggcc tcgcagaacg agttgctgga aacgacgtgc cgggaggtca cgatcagccc
    29761 gtcgtcgctt acggcgaacc cggtcgcgct cgccaggtgc cagcgcgcgc acttcttgca
    29821 tttatacacg gcgcccacca ggacgacgct gtcgcggcag gaggcgtaga tctcctccgg
    29881 cgattgaacg gcgccggagg aaggcagggc ctcgagggcg cagggcttga aggtcttcgc
    29941 ctgctcgatc agggctttca tgggcaccgc gcttttcgcc tgcaccagct tgccgagccg
    30001 cttcgcgaac cggtttatcg tctggtagcc gtcgacgtat tccgggttcg gcgccgggtc
    30061 ggccgccgcg ggcaggatcg cggccaggag cagcgcgagg atcgggcggg cgtgtttcat
    30121 gggtttctcc ggtcagggcc gcgaggtgat ctccacggcg tacgcgatca gggtcacgaa
    30181 agccagcagg ccgaggatgc ccaacagcgc ggtgaggaac tgcgccgcgc gatcgggcag
    30241 tggcgggacc cggccgaagg ccttccaaaa ccggagcagc gtgggcagat gttgctcgga
    30301 ccacgtgatg gccggctggg ccggcgtcgc tgtctctggc tcggtcacgg cggcctcctc
    30361 gcgaaggata cgaccgtccg gggccgcgcg gcgttcattc ttttccagat accgcttgac
    30421 cgcgtccccc accggcagga atgatattcc tcaacaggat tgcctcccat gaaaaagcgc
    30481 gccgtttcac ctccccccgt gtcggtcggt cgcctgcagg aggtcgcgtc cggcctgccg
    30541 ccgaaaaccg tggtcatcgt cggcggcgac cgcaaggagg acctgcacgt cgcccggagc
    30601 cttcgaggcc tgcccttcgt gcgccgctgc gtgctggtcg gcgacggggc cgccatccgg
    30661 gacgccgcgc ggctcgtgga cctcgcggtg ccggacgagg acatcctggc gaccggcagc
    30721 cccgaggaaa cagcccgccg aaccgtggac ctggtggagg agggcgtggg cgagatcctg
    30781 ctcaagggca acatctccac gcccgtcctg aaccgcgaaa tgcgccgcat ccgcagccgc
    30841 gacaccatgt cgctggtgac gatgttccag gcgccgtcac tggccggcgg tcggccggtg
    30901 ctcctgaccg acgccggggt cacgacgctc tgcacgttca gccggatgac tgggatcatc
    30961 cgtaacgcga tcgaggtggc gcagtacgtc ctgggcatcg cgcgcccgcg cgtggcgctg
    31021 ctctcggcca acgagaaggt catcgagtcg ctggcctcca cgaagatggc ggccgcgctc
    31081 gcggggaaat actgggagga cgccgtcgta tgcgggccgc tctcgttcga cctggcgacc
    31141 gaccccgagt cggtgcgcat caaggggctg gccgcctccg aggacccggc cttccgcgag
    31201 gtcgcgggcc gcgcggacat cctggtctgc cccggcctgg atgccgcgaa catcctctac
    31261 aagacggtca tggccatgac gcagcacggc ctcgccgcga cggccgggat caccgccggg
    31321 gtgaaggttc cgtacatcat cctctcccgg gccgacgggg aggcgacgaa gctggattcc
    31381 gtggcgctct gctgcatcta cgccgagcgc cggaaacaga aacagctgga gcaggagcgg
    31441 cgcgttgccg tggcgcggca ggaggaacgg gtcttccacg tcctcgccgt caacccgggc
    31501 tcgacatcgg tgaaggtggc gttgttccgg aacgcggagt gcctggacag caacgagttg
    31561 tcgcacccgc acaggagccg gctggcgggc gcggccttcg acgaggaggt cggccgctac
    31621 ctcgagttgg tgaaggcctt cctcaaagcc cacgcggaac tccggctcga cgccgtggtc
    31681 ggccgcggag gcttcctcca ccgcggcggc cgccagctcg agggcggggt gtaccgggtg
    31741 gcgaccgtgc aggacgggcg ggtgcggatc gaggaggata tcgttcgcgg cgtgcgggat
    31801 tgtcccgaga tggaccatgc atcgaacctg ggcatcccga tggcggcgcg gctggccgtc
    31861 gagtacggcg tgcccgcctt cacggtggat ccggtcgtct ccgacgagtt cgacccgctg
    31921 gcgcagctct cgggttacgc gccgatcacg cgccgcagca cggcgcacgt gttgagcgtg
    31981 cgcgccctgg ccggccgggc cgccgaggcg atcggccgcc cgctggagga gatcagcctc
    32041 gtggtcgccc acatgggcgg cggcatcacg atcgccgcgg ttcgcaacgg ccggatcgtg
    32101 gacaacaaca tcgccctgct cggcggcggc ccctttacgc cccagcgcgc cggggccctg
    32161 ccgacgaagg agttcgcgga gctttgctac aacggaaaat tctcgaagga agagctgttc
    32221 gtcgaactca ccaagcgggg cggcctggtg tcctacctgg gcacgtcgga cgtggagaag
    32281 atccaggacc gcataaccca gggcgacacg cgggcgcggg atgtcctcga ggccatggcc
    32341 taccagatcg ccaaggagat cggcgccatg gccgtggcag ccggccatga cgcggaagcc
    32401 atcgtgctgt ccggcggcct ggcccgctcc gaactgatcg tggggttcat ccggaagcgc
    32461 gtcggccacc tcgcccccgt gatggtttac ccggacaacc tcgagatggc cgccatggcc
    32521 gccggcgcgc tccgcgtgct gaccggccgc gtcgaggcca ggacctatgt tctggcggcg
    32581 ccgtgacggg ccgaccccgg ctttcttctg cgcaacctct tataagcaaa taagatgcgc
    32641 ggagcgtgtg gcgccgccgt ctttttgctt ttttatttga acgcgcggcc ccgctgatgg
    32701 tctttccttc tctagaggat ggccccgggt tccgccggag ctgggaggat tgcatgatca
    32761 aggttgagca tgtcgttaag aagtttgggg cccgcgcggc agtggacgac gtgtccttcg
    32821 tcgtggagcc tgggacggtg ctggggttcc tggggccgaa cggcgccggc aagaccacca
    32881 ccatgcggat catcgtcggg ttcttaagcc cgacaggcgg ccgggtggag gtctgcggcc
    32941 gcgaggtggc ggccgaaccg tcgcgcaccc aggcttccat cggatacatg ccggagaaca
    33001 ccccgctcta cgacgagatg acggtcgagg agttcctgcg gttcatcgcg gagctccggg
    33061 gcttccgcgg cgcggagcgc aaccgcaagg ccggcgaggc catggagtcc tgcgcgctgg
    33121 ccccggtccg gcgccaggcg atcgagaccc tgtcgaaggg ataccggcag cggacgtgtt
    33181 tcgcgcaggc gctgctgcac gatccgccgg tgctgctgct cgacgagccg acgaacgggc
    33241 tcgatcccaa ccagaagcag gtggtccggg aaatgattct gcggatggcc ccgcggaaga
    33301 ccatcgtgct gtccacccac gtgttggagg aagtcgaggc cgtctgctcc cgcgtcgtca
    33361 tcatcagccg cgggaaggtc gtggcggaca gcaccccggc cgcgctgaag cagcgcagcc
    33421 gctccttcaa caccctgacg ctggaactgg tggcgcccgc ggaccaggcc gaggcgggct
    33481 tccgcggcct gccggacgtg gagcgggtcg cggtgctcga gcgccgggac ggcggggcct
    33541 gccggcttca gctggcgccg cggaaccagc agccgctggc ctccgccgcg ctggagttcg
    33601 cccgcgggcg gggctggctg gtcacggaca tgcagaacga cggcgggcgg ctggacgagg
    33661 tcttccagca gatcacccgg acggacgacg ccgcgtgagg aggctgaaca tggaatcgtt
    33721 acggcatatc cggtccatcg cgcgccgcga gtggagcgcc tacttcaact cgccgatcgc
    33781 ctacgtcttc atcgtgatct tcctggcgct ggccgggttt ttcacgttct cggtggcgcg
    33841 cttctacgag gagggccagg ccgacctgcg gggcttcttc ttctggcatc cgtggctata
    33901 cctgatcctg gtcccggcgg ccgccatgcg gctgtgggcg gaggagcggc ggtcggggag
    33961 catggaactg ctgctgacgc tctccgtgac cccggcgcag gcgctgctgg gcaagttcct
    34021 ggccgccggg ctgttcctgc tgctggcgct gggcctgacc ttcccgctgg tcctgacggc
    34081 cctgtacctc gggtcgcccg acctcgggcc cgtgatctcg ggatacgtcg gcagcgccct
    34141 gctcgcgggc gcctacctgg ccgtcggcct gttcacctcg gcgctgacga agaaccaggt
    34201 ggtgagcttc gtgctggcgg tcgtgctcgg cctgttcctg atcctcgcgg gcttcccgcc
    34261 ggtgacggac ctgctggccc agtgggcccc cgagtggctg gtcaacggcg tggcggcctt
    34321 cagcttcatg ccgcatttcg atacgctcca gcgcggcgtg ctggacctgc gcgacttcgc
    34381 ctacttcgcg agcgtcatcg gcttcatgct gctggcgacg acgctggtgg tgcggggcca
    34441 gacctcgagg gcctgaccgg acaggagatt cgaaccatga gcaaaaagtc ccttcgcatc
    34501 gggttgagcg gcgccgccgg cgcgctcatt gtgctggcca ttctcgtcgc ggtcaacgtc
    34561 atcctgggcg cggcgcgcgt ccgcaaggac ctgaccgccg aacgcatcta cacgctttcg
    34621 cccggcaccc gcgccttcct gggcggcctg gagcgcggcg tgacgctcaa gctgttcttc
    34681 tcgcgcggca acgagaacct gccggtcggc ttcaagcagt acgcccggcg cgtgacggac
    34741 ctgctgcgcg agtacgaggc gcacggcggc gggcgcgtgg cgctcgagac ctatgacccg
    34801 cagccggatt cggacgagga ggagtgggcc cagcgctacg ggctcgccgg ccagaacctg
    34861 gaccggtacg gcatggagcc ggacttctac ttcgggctgg tcgcggtctc gggcacgcgc
    34921 gaggcggccc tgccgttcct ctcgcccggc gccgagccgc agctggaata cctggtgacg
    34981 cgattgatcc acgaggtcac gcgggctggc aagccgaaga tcggcgtctt gagcgccctc
    35041 cgcgtgatgg gcccgcccca gcgcccgctc ggcatgacgc aggacgaggc cggcggctgg
    35101 cagatcatga cggaactggc ccggcagtgc gaactggtgc cgctgaccga cgaactggag
    35161 accgtgcccg ccgacatcga gaccgtcctg gcgatccatc ccaaggggct cccggacaag
    35221 acgctgttcg cgctggacca gttcgtgctc cgcggcggcc ggctgctggc cttcgtcgat
    35281 ccgttgtgcg tcgccgaccg cgagtcgggc ctggcggccg atccctacgg cacgggcccg
    35341 gcgtcctccg acctcaaccg gctgaccaag gcctggggct acgagctggt cccgggtcag
    35401 gtcgtcgccg acgtcgaggc ggcgagttac gtgacgttcg gcgggcgggg ctccgagcgg
    35461 ctgcccacgt ggctctcgct gcggccggac aacatcaacc gggacgagat cgcgaccgcc
    35521 gcgctggaaa acctgatgat gccgttcgcg ggagcgttca agggcgccgc ggccgagggc
    35581 ctgacggcgg tcacgctggc gcagacctac gatgaagccg cgctgctgcc cgcctttcag
    35641 gccgcgcagg ccggcgtgga gaaaatgcgc gcggcgaagc ccgagggctc cctcgtcctg
    35701 gcgctgcggc tgcagggccg gttcaagacg gcctttcccg acggggcgcc ggccgcgttc
    35761 ctgaaggaag gcgaaaagga cagcgtcgtg atcctagtgg ccgacgccga catgctgcac
    35821 gaccggaaca gcctgcagga aatgaatttt ctcgggcagc gactgctaca gcccgtcaac
    35881 gacaacttga gtttcggcct gaacatggtc gagcagctca ccggcgacga agcgctcatc
    35941 ggcctgcgca gccgcggcac atacggccgg cccttcgacc gggtcgtcga gctcgaaaag
    36001 caggcgcagc agcggtggca ggaggaggaa ctcaaactgg tcgagaagct ccgcgaagcc
    36061 caggcgcggc tcaacgagct gcagcgctcg aaggacgaag accagcagta catcttgacc
    36121 cccgagcaga agcgggagat cgagaatttc cgccaggagc ggttcgagac gcagcggcag
    36181 cttaaggaag tccgaaagag cctgcgccgc gatatcgagc ggctcggcct gaaggtgaaa
    36241 atactcaaca tggcggcgat gccggcggcg gtggcgctct tcgggctgct gcacggctgg
    36301 cgccggaaga agaaggcctc gtcgtaacgg ccccggaagg aggaaagccc atgtctccaa
    36361 aaactctcgc gcttctgctg gtgatcctgg tcgcgctcgt ctggtggcag gggcatcccc
    36421 gggcgggcgg gacggagcgg gcgcccggtt cggccatcct gcccggcctc gacgtcaatg
    36481 cgattgaccg gatcgaggtg gcttccgcgg ccgccacctc cgtggtcgag cgcagggacg
    36541 ggcagtgggt ggtgccgtct ctgttcggcc acccggctga ttttcaaaag gtggcggacg
    36601 cgctgcgcgg cctggcggat ctcaaggtcg ggcaggtggt ccgggacgcc ggccagtatc
    36661 cggaggaatt cggcctggcg gcgacgagcc ccgtgttccg ggtcgcgctg tccggcgctt
    36721 cgggcttcca cgccgcgctg gaactgggcg cgacgaagcg ggagggcgcc caggagggct
    36781 tcgcgggata cgagtccggg cgctatctcc gcgcggcggg cgggaccgtg gtcctggtcg
    36841 ctgacccgct gcaatccttc gcggacgcgt cgggagagtg gatccggaag gagttgttga
    36901 acgtggcgcc gcccgagctg gatcgcgtca cggtggcggt tttcaacgcc gagcacgtgt
    36961 tgctcatcaa ggggtctaac cagtacgagg tcgaaggcct gaaggacggc gaggaaacgg
    37021 atccggccgg cgccgcccgg ttggttggag ccctgcaatc cctgtcctgc ctgaccgtgg
    37081 ccgacccggc gctgaccgac gaggccgcgg gtttcggcgc gccggaccgc tgcacggtgc
    37141 tcgcgcgcaa ccgcatggaa tacacgctgc aactgggcgg cgcgggcccg gaggcgggtt
    37201 cgcggtacct ccgcatcgac gtggcctacc gggagccggc gccgccgact ctcgaacaag
    37261 tggctaagag cattccggtc gcggcggaga gcgccgggtc ggacacgggc cgcgtggaga
    37321 aggttcaggc cgaataccgg gagcgccttc aggcccacga agccgagatc gccgcccgcc
    37381 gcgaggaaag gggtcgattg gagaagcggt tccgcggatg gacctacgtg atcccggctg
    37441 gaaccgccga atccctgatc ctgccgcgcg ggcagttggt gaaggccaag cccccggtgg
    37501 cgccggcggc cgtctctccg caggggcagg atcagggccc gccgtgacgg ttacgccatc
    37561 caattgacgg gcttgtccgg gttccacttg accgagatct tcttcagctt cgtccagaag
    37621 ccgaatgagc tcttgcccgt aatgtcgccg cctccgacga tggaattttt ccagccgccg
    37681 aaggagaacg gctcgcgcgg caccgggacg ccgacgttga cggcgaccat acccgcctgg
    37741 atcttcttgc agaaaatttc tgccgcgcgg ccgctctgcg tgaagatcga gccgccgttg
    37801 ccgtagggtg aggaattggc gatatccagg gcctcgtcca gcgtgcgggc ccgaacgatc
    37861 gtgaggatcg gcccgaaatt ctcctccatg gcgatttccg agccggcctt cgcctcgatg
    37921 atggtcgggc ccaggtactg tcctttttcc tttcccgcgg gcggcttcgc cccgcggcca
    37981 tcgagcagga tattcgcccc atccttctcc gcgcgatcga tgtagccgat gatgttggcc
    38041 ttcgcctcgg tattcacgat ggcgctcatg ttcacgccgg ggacgaactt cttcgactcc
    38101 tcgaccagct tggcgatgat gggatccacg tcgcccacgg cgaccaggac gcaggcggac
    38161 atgcatcgct ggcccgcgca accgaggtac gaggctgcga tgttggccgc cgagttggcc
    38221 ggatgcgcat cgggcaggag gatcagatgg ttgttcgcgc tgcccagggc cagaacccgc
    38281 ttgaagtttg cacagccctt cgcatagacc atctgggcca cctttgtcga gccgacgaac
    38341 gtgatgccct ggatgttagg gtgctccaca atggcgttca cgacctcgcg cccgccgcag
    38401 acgatattga gcacgccgtc cggcaggccg gcttccttca tcatctctcc gattttcagg
    38461 accgtgtagg gcgcctcgcg cgacggcttg aggatcatgg tgttgcccaa gaccaacgcg
    38521 ttcggcaggg tccagtgtgg aaccatgtgc gggaaattga acggggtgat cgaggcgaca
    38581 acccccaggg gaacgcgcac gtcgtggcac tccacgcccc ggctgacctc ctcgaaatcg
    38641 ccggtggcca tctgcggcat cgagcaggcg acctccgtca actcgaggct cttcccgact
    38701 tcctggagcg cttcggcggg gatcttgccc atttcgtggt actggatatc cgcgatctcc
    38761 ggcaggcgtt tgctaagcaa ttcgtagtac ttgtagaaga cgccggtgcg ctccttgatg
    38821 gtccactgcg accagtcggc gaacgccttc ttcgcggcct gcacggcctt atcaagttcc
    38881 ttggcatcgg agaggggcag gctggaaagc aattcgccat ccacaggact gtaccgctcc
    38941 atccgcggca cgccggggtt gctggattta ccgtcgatga agttgctcaa attggagtat
    39001 ctcatggtcc gtcctttcgt gaatacgggg acaacgagta gcactcgggc ggcggtttca
    39061 caacctcaaa agccgcgagg tctcctgata ctcccggcgg tcgggcccca catgctcaca
    39121 cttctagaag tatgagcatg gaagacgata cgccgaatta cgtgtaatga tacatcatat
    39181 taataatgag tataatatgt gtaatttgcg catgctcaca cttctagaag tgtgagcata
    39241 gttgtgttgc atgggtattg ccttcgtccg cgctcctcct tggttccatt gtctgcgggc
    39301 ccttgcggcc gccacgaatc cccgtgatca aatccgcatg tccgccggca tcggctcatg
    39361 ctgtcatggc cggagaggat catgccggct acggcgaaaa tccctttaca ttccggcggt
    39421 ttttccgatc atggggcgac aggagaaccg tatgcccaca cgcgcaacgt gaacaggagg
    39481 cccaccatgg cgcgcatcgt caaagccggt ctgatccaat gctcgctgcc cctccacgag
    39541 ggcgaggggt cgatggagga aatcaaggac gcgatgctga agaagcacct tccgctgatc
    39601 gacgaagccg gtcggaaagg agtgcagatc ctgggattgc aggagatttt caccaccccg
    39661 tacttctgtc cgtcgcagga ccccaaatgg ttccgcaccg ccgagaagat accggacggg
    39721 cccacggtcg cgctcatgca gaagtacgcc gcgaagcatc agatggtgat cgtcgtgccg
    39781 atctacgagg aggcgatgac cggcgtgtac tacaacaccg ccgcggtgat cgatgccgac
    39841 gggacgtacc tgggcaagta ccgcaaaaac cacatcccgc aggttggcgg cttctgggaa
    39901 aaatattact tcaagccggg caacctgggc tacccgacct tccagacccg ctacgcgaac
    39961 gtcggggtct acatctgcta cgaccgccat ttccccgagg gcgcgcggct gctcgcgctc
    40021 aacggggcgg aaatcgtgtt caacccctcc gccacttgcg ccggcatttc ccagtacatc
    40081 tggaaactgg agcagccggc acacgccgtg gccaacggca tcttcatggg ctgcaacaac
    40141 cgcgtgggcg tggagcgccc gtggaacatc ggtcggttct acgggaccag ctacttcgtc
    40201 gatccccgcg gagaaatgct ggccgtcggc agcgaggaca aggacgaact cgtggtcgcc
    40261 gacctggacc tcgaccagat ccgggagatc cgcgacaagt ggcagttctt ccgggaccgc
    40321 cggccggaaa cgtacgggga catgacgaaa ttgctgccgt gagttccgcc gggaacggag
    40381 aaaaggcaca ccggttcaag aaggagaatc ccatgagcat cgtgatcaag ggcgggacga
    40441 tcgtgaccgc gggcgacctc tgcgcggcgg acctgcggat cgacggcgaa cagatcgtgg
    40501 agatcggccg ggaccttccc gccaaggccg gcgatacggt cgtctccgcg aagggccgct
    40561 acctcttccc gggcggtgtg gatgttcata cgcatttcga actgccgttc atggggacgg
    40621 tcagcgcgga tgacttcaag accggccccg tcgcggcgct gtgcggcggg acgacgacct
    40681 acatcgattt ctgcatgcag gccaagggca agccgctggc ggcctcgctg gcggcctggc
    40741 atgagaaggc gaagaaatcg gcggcggact tcggcttcca catgggcatc gtggattaca
    40801 cccccgcggt cgcggcggaa atcccggcgg ttatcaagca gggcgtctcc tccttcaagt
    40861 tcttcatggc ctacaagggc gcgctgcaga tcgacgacgg gcagatcatc ggcatcatgg
    40921 agaccgtcgg ccggaacggc gggctgtcca tggtgcacgc cgagaacggc gacatgatcg
    40981 acacgctgtc gcggcgcttc atcgccgagg gcaagcagac gccggaatac cactggctcg
    41041 cgcacccggc gatcgcggag gaggaggccg tgcaccggat cgtcgagttg gcccggttca
    41101 cggagcagac gatctacatc gtgcacctct cgagcgccga cgggttggag aaggtcaagg
    41161 aggccgtggc gcgcggctac ccggtcatcg cggagacgtg cccgcagtac ctgctgctgt
    41221 cgagcgacct ctactacagg ccgaacttcg agggcgccaa gtgggtcatg tcgccgccgc
    41281 tgcgcccggc ggaccacctg gagaaaatgt gggaggggct gggccggggc ttcatcaaga
    41341 ccgtggcgac cgaccactgc tcgttcaact tcaagggcca gaaggagatg ggccgcgaca
    41401 gcttcgcgaa aatccccaac ggcatcccgt ccgtcggcga ccggttcaac ctgctctaca
    41461 cgtacggcgt ggggcagggg cggatgagcc tgacgcggtt cgcggacgtc gtggccacgg
    41521 cgccggcgaa gatcttcggg atgtaccctc ggaagggcac gctggcggtc ggctccgacg
    41581 cggacgtggt gatcttcaac ccggaggcca agggcgtggt gtccgcgaag accacgaagc
    41641 ataacgtgga ctacagcgcc tacgaggggc tggaactgaa gggcctgccg gagtcggtgc
    41701 tgctgcgcgg caagttcgcc gtgcgggatg ggcagtacgt gggcgagcag ggggccggcc
    41761 ggttcatcgc ccgcgggccg gcggggaaaa tggtgtagcc gcgatcggtg accgcgggct
    41821 acggttggca atcgcggcca cagcaaaggc ttaaaaaatg aacaaagaca aaatcgccga
    41881 gatcaagcag tgggacgccg agcacgtcat ctatccttgg aagacccagg gctggtatcg
    41941 ccccatcctc atggagcgcg ccgagggcat ccacatgcat gacgccgagg gcaagcgcta
    42001 catcgacttc tgctccggcc tgctgaacat caacatcggc cacgggaaca agcacgtact
    42061 ggacgccatg aaggcacaga tggacaagct gtgctacgtg gcgtccgtct ttgccaccga
    42121 acccaaggcc aagctggcca ggatgatttc cgaggtgacc ccgggcgacc tggactacgt
    42181 cttcttcacg aacgccggcg cggaggccgt ggagaacgcg atcaaggccg tgcgctggtt
    42241 caccggccgc cagaagatct actccaactg gcgcggctac cacggggcga cgtccggcgc
    42301 gatcacgctc accggcgacc cgcggcgcat cccgtgcgag ccgggcatcc ccggcgccgg
    42361 caagttcttc gggccgtact gctaccgttg cccgttcggg tacaaggacg agaaaagctg
    42421 cgggatccag tgcctggaaa cgctgaagac gcagatcatg ctcgaggggc ccaagacgat
    42481 cgcgggcatc ttcatggagc cgatcgtcgg caccaacggc atcatcatcc cgcccgtgga
    42541 gttcatgaag ggcgtgcgga agctctgcga cgagcacggc atcctgctgg tcatggacga
    42601 ggtcatggcg gggtggggcc gctcgggcac gtggtttggc ttcgagcact tcgggatcgt
    42661 gccggacatc atcacggcgg cgaagggcct gacctcgggc tatgtccagc tcggcgtgat
    42721 gatctggagc aagaagctct gggacttctt caaggacaag ccgttcatca gcggcctgac
    42781 ctactacggg catgccctgg cctgcgcggc gggcgtggcg aatctggagg tctacaagag
    42841 cgaagggctg atcgagaagt cccgcatcaa cggcgactac ctgaacaaca agctctggtc
    42901 gctctacgag cggcatcctt cgatcggcga cgttcgctgc aagggcctct gggcctgtct
    42961 cgagctggtg gccgaccgca agacccggga gccgctggcc ggctacgccg acgcgaagcg
    43021 caacgtcatg ggcgagttca ccaagcgcct cttggagatg ggcctctggg cgttcgccaa
    43081 gtgggactac atcttcctcg cgccgccgct gatcatcacg ccggagcaga tcgacgaggc
    43141 cgtggcgatc ctcgacaagg cgctggaata cccggattcg ctgctcaagg gctgaccgga
    43201 cgccgaggag cgccgttatg gatctgcacc agatcatcag cttcttcggc accgtcattc
    43261 tgctcttcat cggctggctg ttctgcaagc accggaagag cgtccattgg cgcacgatcc
    43321 tgtggggcac cgccctgcaa ctgatcttcg ccctgctggt cctcaagacg ccgcccgggc
    43381 gctggctgtt caatttcatg aacgacgcca tcgtcaagct cctgtcgttc caggaggagg
    43441 gcggcaagtt cgtgttcaac gcgctgtcga tcccggtggg gcagccgggc tcgatgggct
    43501 tcttcttcgc cttccaggtc ctgaccacga tcgtcttctt ctcgtcgctc atctccgtcc
    43561 tgtactacct cggcatcatg cagaaggtgg tgcagttctt cgcgaggatc atgttcgtca
    43621 cctgccagac gtccggcgcg gagaccctgt ccgcgtcggc caacatcttc gtcggccaga
    43681 ccgaggcgcc gctgctggtc cggccgtaca tcgaggacat gacggagtcc gagctgatgt
    43741 gcgtgatgac cggcggcatg gccaccgtgg cgggcggagt gatggccgcg tacgtgggga
    43801 tgctgaaggg ctatttcccg gacatcgcgg gccacctgct ggcggccagc gtcatgtccg
    43861 cgccggcggc gctggtgatg gcgaagatca tgatcccgga gaccggccac ccgaaaacga
    43921 ggggcacggt ggccatcgcg tacaaggacc cgagcgtcaa tgtcatcgag gccgcggcca
    43981 acggcgccac gatgggcatg cacctggcct tcaacgtggg ggccatgctg gtggccttca
    44041 tgtcgctgct ggcgatggcc aactggatcc tccagcatgc gatcgcctgg ttcggtccgg
    44101 agggcgtgac gctgcagcgg cttttcggat gggccttcgc gccgctggcg tgggtgatgg
    44161 gcgcgccctg gaaggactgc caggtgctgg gccagttgat gggcgagaag accgtgctca
    44221 acgagttcgt cgcctacacc agcctgatgc agcacgccgc cgcgcacccg gggggattgg
    44281 cgtaccgctc ttacgtgatc gccacgtacg ctctgtgcgg cttctccaat ttcctctcca
    44341 tcggcatcca gatcggcggc atcggcgcga tcgccccgag ccgcaagcgg gacctggcga
    44401 ggctggggat ctatgcgctc atcggcggct cgctggcctc gttcatgacg gcgaccatcg
    44461 cgggcatcct ggtgccgtaa gcccggcgtg gcagccgcgg tcgttgaccg cggcatctct
    44521 gcgcggagac cattcgccac tcaagacggc tgccggcgca gcgaccatcg ccgcagggtc
    44581 aagatcatga cgccaacggc cagcagcatc agcacgatag cggtcaccgt caaagggtaa
    44641 ttccggttcg ggaggtactg gcccgtcagc agctgccaca gcgagaacag ggtcgtggcc
    44701 atcatgaaga cagccggcgc gacggcgaac cacgagggcc tggagcgcgc ggccagccag
    44761 acggagacga cgatcagggt cagcgccgcc atcagctggt tggcgctgcc gaagatgggc
    44821 cagatgtgct tgaacgtgtt cgtgtagcag aggacgaaca tcaggcccga caccaccacg
    44881 gcgttgaaca ggtggctgcg cagcaggctc ggcgcgcgct tgaacagcac ggcccacaac
    44941 tcctccagca ggtagcggtt cagccgggcg gccgtgtcga gcgtggtgac gatgaacccc
    45001 tcgaccatca gcatgccgaa caccgttccg tattccatcc gcaggccgag gccgcgatgc
    45061 acgagcgagc ccatgcccag ggcgaaggcc agcacgggat tgctcccgac gcccttggcc
    45121 gcgggataga ccagcccctg gtactgctcc agcgacaggc cggtcgccac ggcgacgatc
    45181 acgagcacgg ccaggaccgc ctcgaggagc atgccgccat agccgacgcc ccgcgccgcg
    45241 cgctcgctcg acagctgttt cgctgtcgtg ccgcccgtga ccatcgcgtg gaaacccgag
    45301 atcgcgccgc aggcgacggt gatgaacagg aacggccaga ccatgccgag ggattgccgg
    45361 ccgccggcca ggttccaggc cggcatgttc gggtccgcgc cgccggcgcc gcccgccgcc
    45421 gacccgatga tcaggatggc catgccggcg tagaggagga acgagttgat gaagtcgcgc
    45481 ggctgcagga tgacccagac cggcagcccg gaggcgagga tcacgtagaa cgtcaggatc
    45541 agcatccagg ccatggggga aagtgtcaac gggtacttca gccccgccgc gatcgaggcc
    45601 aggcagatgg ccatggcgag ggggaacatg ctgcgcatgg tcatgccgcg gcggtagacc
    45661 agaaagccga tcagcggcgc gccgagcgtg atgatgatca gcgacgtcga ggccacgccg
    45721 ccgaccttgg ccctcgccag gccgtccgcg ccggcgacca cccgcaggaa cgtccgcccg
    45781 gcctcaatgc gaagctgctc cagcgggacc agcgaggtca gggccgtggc cgacaggccc
    45841 aggaaggccg aggtcagcaa cagcaacatg atgatcgaga acgacacgac caggaagaac
    45901 ccgaaccggc ccattgtccg gtttgcgacc tgcgccatgg agtggccctg ctcgcgcatg
    45961 ctgacgaaca ggctcgcgta atcctggacc gcgccgatga acacggagcc gagcaccacc
    46021 cacagccaga ccgggatgta cccgtagatc agcgccacgg tcgggccgac gatcggcccc
    46081 gcgccggcga tggacgcgaa gtggtggctg aacacgaccg gtgtcctgct cgggacgtag
    46141 tccacgccgt ccttcatcgt gacggacggc ggcggctcct ccgtcccgcc ggtcagcgcc
    46201 cgctggagat accgggaata aaagcgatag cccaggaaga acaccggcag cgccacgccc
    46261 accaaccaga gcacgttcat gaatgcgcct cctgcgcgcc gggcttatga cctccgctgg
    46321 gtttcgagtt ccttgcccag cgtgacggtc tcggcgcgcc tgtacgattt cggcatttcc
    46381 ttaaaggtga tcaggccgtc cacggggcag gcgaagctgc agaccatgca gctcaggcac
    46441 tttttctcgt cgagcgccgg gcgccgcttc ttcgcgtccc agtcgagcgc ccagccgccg
    46501 gcgtcgcggc agacgacgta gcactggccg cagccgacgc agcggtcgag gtcgtaccgc
    46561 gcgatgccct gccgcgcgag gtcgaagtcg tgcgtcgcgt gcacgttggg cagggccttg
    46621 ccgatcagct ccttcaggct cttgaccccc ttgatcgtca tgtaatcgga cagcccctcg
    46681 atcatgttct ccacgatgcc ctggccgtag tggatgatgc ccgtggtcac ctgaatcgtt
    46741 gttgccccga ccagcaggta ctccagcgcg tccacccatg tctcgatgcc gccgatgccg
    46801 gagagcggga gttgcaggtc atggcacttg gccagttcgg agatgcagcg caggccgatg
    46861 ggcttgatcg ccgggccggt gaacccgcac gctgcgccct tgccgaacac gttcgggctg
    46921 ggcacccagt cgtccgcgcc gatccccgac aggccggcca cggaattgat cgccgcgatc
    46981 ccgtccgcgc cgcccttctt ggcgtacatc gcgggcacgg tgatgtccgt gatgttcggc
    47041 gtcattttgg cgacgatcgg tatcttgcag gaatttcgca ccgtttccgt gaatttttgc
    47101 accagttcct ggcactggcc gaccttggcc ccggagccct cgacggtcat gtgcgggcag
    47161 gagacgttga cttccagcat gtccgcgccc gcgtcgacgc aggccttggc caggtcagcc
    47221 cattcctggt tggagaatcc catgatgctg gccatgacca ggtggtccgg ccagcgcttc
    47281 ttgaggtaca tgatgtccag caggttggcc ttgagcccgc ggtcggaagt ttgctcgacg
    47341 ttctgcacgc ccagctgccg cttgcccgcc ggccggtacg ccgccatgcg gggggagggg
    47401 tggatgatcg ggatccggtc cgacacgacg gtcttgtagg ccacgccgcc ccagccggcg
    47461 tcgaaggcgc gcccgaccat ttccgcgctg ttcgagaccg gggacgagga cagcatgaac
    47521 gggttcttca tcttgaaccc gaggaactcg acggagagat cggcctgctt actcatgggc
    47581 gttctccttc ttcgcgaagc gtttcatgat ggcctgggcg gcggtcttgc cgtcccggac
    47641 ggcctcgatg atcagcgccg ggccgcggac gatgtcgccg cccgcgaaga cccgcttgac
    47701 cgacgtctcg ccggtgtccg ggttgaccgt gatcaggttg ccgtccgaca gcttcatgga
    47761 tcgagaccac tcgatcgagt cacgggccgg ttgcgcgccg atcgcctcga tggcgacgtc
    47821 aatgggcagt tcccagttgg agcccgggat ctcgacgggc ttgcgccggc cggaggcgtc
    47881 gggcttgctc aattccgtgc gcaccagtcg gatggccttg agccggccct tcgcatcggt
    47941 cgagtaatcc accggctggt tcagcagcaa caggtgcatg ccttgccgca gggcgtcctg
    48001 cttctcgtcc tcctcggccg gcatctcgtt gtacgagcgg cggtagacga ggtagacgtc
    48061 cttggcgccc agcttgatcg cggattccgc gcagtccatg gccaccgacc cgccgccgat
    48121 gatcgcgacg cggcggccct tgagctgttt cccgatggcg gcgaaccggc cctccttgag
    48181 cggcgtcagg aaatcggtcc aggcgaacac gccctggcgg acctttgctt tgactttcag
    48241 ccgcagcggc tcccagcagc cgggcgcgag gaagacggcg gcaaatccct tcttcagcag
    48301 cgactcgacg gcgcccttgc cgcggatcgg cttgctgcac tggatcttca cgccgagcgg
    48361 ggccaggtct ttcagttcgc ggtcgatgaa gtccggggag aagcggtagg acggcacgcc
    48421 gtagcgcagc acgccgccgg cccgggcgcg ggcctcgaag accgtgacgc cgaacccggc
    48481 cttggccagt tcggcggcgc agctcaagcc ggccgggccg gagccgacca cggcgatttt
    48541 ttccttccgt gccgatcctt tttcaagggg attgaagccg gtcgcccacg cgtgctcgac
    48601 gaggaaccgc tggatcttgc cgatctggat cggacggtcc aggcccgcgg cggagcattc
    48661 cttctcgcag agctgcgggg tggggcacag ttcgccgcag ctgccgccga ggatgttgtt
    48721 ctgcttgatc gtgcggacgg cgccggtcag gttctggaag cggagcttgc ggatgaaggt
    48781 cgcgggatcg gtcccgccgg ggcagccctt ggagcagggg gcgtcgtggc agagcaggca
    48841 gcgctgggcc tccgccacgg cttgtccata gtcgaagccc tttttcatgg acgcaaacgg
    48901 ggctggcgct ttcggcatgg gccctcctca cggtttcagg cagcctgtat caacactatg
    48961 cgcgccgtat gctacggttt tccgcggtcg gcgtacagga ttttgtcagg gcgcatgcag
    49021 ctggatccgg ctctccggca cgcggctgtt tggagccggg aagcctgatc agcctctccg
    49081 tcactccctg cgttgacgct gtcggcgcgt ccgggtatac aatgcatcgg gggaggagcg
    49141 ggcagggtgc gacacccgtc cctggatggt ctgcagaggc acaacgtgaa attgtttcat
    49201 gtacggctcg cctttggtct tctgggcgtt ggcgcgttcc ttgttcctgc ccatgccgtc
    49261 actcggtacg tagccggtaa cggcgggcac gttccgccct tcaccaattg gaccacggcc
    49321 gcgacatcca tacaggccgc ggtcaactcg gcgacgaacg gcgactggat cctggtcacc
    49381 aacggggttt atggcggaaa cgtgctgatc agcaacaaga cgatttccct ggtgtccatg
    49441 tacctgtaca gcggcgccat ctcggatgcg gagagcaccg tcatcctcgg aaccggttcg
    49501 ggcggcgccg tgcgcatcgt gaacggcgtc ggaacgagtg gccggttcat gggcttcacg
    49561 atcaccaacg gatacgcgaa cggatcctcc acgggcgagt acgacagggg cggcggtgtg
    49621 tacgtggcgg cttccggccc gcgcctggag gcgctgcgca tccggggcaa ccgggccaca
    49681 tcgacgggcg ggggattgta cctggagcat gcgaccagcg tggtgaataa cgtggtggtg
    49741 gagaacaatc aatccagcgg gggttcggcg ggggcggggt tctactatgg gaaaccgagc
    49801 ttggagaacc tggtggtgcg ggacaacgtg gcgtcgagct cggacggcgg cggcatcctg
    49861 ttctaccact gcaacgcgac ctgccggaac atgctggtgg tgaacaacag cacgaccgag
    49921 gacggcgggg ggatgcactt cgacgacagc gacgtgctgc tggagaacac gacggtgtac
    49981 gggaaccggg cgcggacggg cggggggttg aacatctcgt acagcagctc gccgcggctg
    50041 gtgaactcga tcgtatggag caacacgccg gacaacatcg ggttctgcac gcagtggttc
    50101 gggatggata tccgggtgga ttactcggac gtccagggcg ggtcgaacgg cgtggtgacg
    50161 tacgggaagg ggccggtgta ctggggggcg gggaacctgg actccgaccc gatgttcgac
    50221 gccggggatt tccagctatt ggatggttca ccgtgtgtca acgcggggga caaccgcgac
    50281 tggatgacgg gagcggcgga cctggcgggg gcgccgcgga tcgcggggcc gaaggtggac
    50341 ctgggggcgt acgagaaccc gaacggggcg tcggtgccga cggtcagcgt gccggcgacg
    50401 ccggcgggac ctgcgtcggg gctggcgggg ccgtcgctgg cgtacgtgac gggcggttcg
    50461 acctcgagcc tgggcggcgg ggtggaatac cggttcgact ggggcgacgg gaatctttcg
    50521 gggtgggacg cgaacacgga ggcggctcac gcgtggtcgg cgacggggct gttcgccgtg
    50581 gcggcgcagg cgcgcagcgc ggtgagcgcg gacgtggtgt cggcgtggtc gggcgcgctt
    50641 ccggtatgga tcaccaacgc gccgccggtg ccggacgatc actacgtggc gctgacgggt
    50701 tcggacgaat acccgtacac gaactgggcg atggcggcga accgggtgca ggacgcggtg
    50761 gacgcggcga cgcacgggga cacggtgtgg gtgaccaacg gcacgtacga gggaaacgtg
    50821 cgtttcaacg gcaagaacat ccgggtgaca tccctgttca cggtcagtgg ggatgctgcg
    50881 gacatcgaga cgaccgtcct gcagggcata ggttcgggca gcgtggtgcg gatcacgagc
    50941 ggggagacgg tggattcggc gctgatcggg ttcacggtca cggggggcta cgcggacggg
    51001 cctgcggacg gggagtatga ccgcgggggc ggcgtgtgcg tgcgattttc gcggccgcgg
    51061 ctggagcatt tgcggatcgt gggcaaccgg gccacatcga cgggcggggg attgtacctg
    51121 gagcatgcga ccagcgtggt gaataacgtg gtggtggaga acaatcaatc cagcgggggt
    51181 tcggcggggg cggggttcta ctatgggaaa ccgagcttgg agaacctggt ggtgcgggac
    51241 aacgtggcgt cgagctcgga cggcggcggc atcctgttct accactgcaa cgcgacctgc
    51301 cggaacatgc tggtggtgaa caacagcacg accgaggacg gcggggggat gcacttcgac
    51361 gacagcgacg tgctgctgga gaacacgacg gtgtacggga accgggcgcg gacgggcggg
    51421 gggttgaaca tctcgtacag cagctcgccg cggctggtga actcgatcgt atggagcaac
    51481 acgccggaca acatcgggtt ctgcacgcag tggttcggga tggatatccg ggtggattac
    51541 tcggacgtcc agggcgggtc gaacggcgtg gtgacgtacg ggaaggggcc ggtgtactgg
    51601 ggggcgggga acctggactc cgacccgatg ttcgacgccg gggatttcca gctattggat
    51661 ggttcaccgt gtgtcaacgc gggggacaac cgcgactgga tgacgggagc ggcggacctg
    51721 gcgggggcgc cgcggatcgc ggggccgaag gtggacctgg gggcgtacga gaacccgaac
    51781 ggggcgtcgg tgccgacggt cagcgtgccg gcgacgccgg cgggacctgc gtcggggctg
    51841 gcggggccgt cgctggcgta cgtgacgggc ggttcgacct cgagcctggg cggcggggtg
    51901 gaataccggt tcgactgggg cgacgggaat ctttcggggt gggacgcgaa cacggaggcg
    51961 gctcacgcgt ggtcggcgac ggggctgttc gccgtggcgg cgcaggcgcg cagcgcggtg
    52021 agcgcggacg tggtgtcggc gtggtcgggc gcgcttccgg tatggatcac caacgcgccg
    52081 ccggtgccgg acgatcacta cgtggcgctg acgggttcgg acgaataccc gtacacgaac
    52141 tgggcgatgg cggcgggcag catccaggcg gcgatcgggg ccgcggaaga cggggacacc
    52201 gtctgggtcg ccagcgccga gtaccgggag aacatagact tcacggggaa gaacatccgg
    52261 atcgtctcgc tgttcgagtt gaccggcgat ccggcggaca tcgcggggac gattatccgc
    52321 ggaacgggca cgggcaacgt cgtgcgcgcg gtgagcggcg aatccaccaa tgccctgttg
    52381 aagggattca cgatcgtcaa cggccacgcc gtgggcgtcc atccgttcaa tcgcggcggc
    52441 ggcatcctgt gccagtacgg cagcccccgt attgaacatg tgcgggtcac cggaaaccac
    52501 gccgacatcg tgggcggtgg catctacctc gagcattccg ccagcgccct gcgcgacgtg
    52561 gaggtggccc acaacaccgc gggcggcggc ggcgggatgg gttgctacta cggtcagccg
    52621 tccctcgtca atgtgtgcct gcaaagcaat acggctaccc agtccgacgg cggcgggatg
    52681 atcctctacc atgccagtcc gctgatgagg aatgtgttgg tggcggacaa cacggcaccg
    52741 gccaaggccg ggggcatcca tttcgatgcg gcgaacccgg tgatggagaa tctcaccgtc
    52801 gtaagcaact ccgcggccca cggcggcggt ctgaacgtct cctacagcag cgcgccggtc
    52861 cttcggaatt cgatcatctg gggcaacagc ccgcagcagg tggaattcga cgactggtgg
    52921 tttgccatgg cgctcaccgt cgagtactcg gacctccagg gcggcctggc cgccatagag
    52981 acgcacgggt tgggtccggt gcactggctc gacgggaata tcagcgccga tccggtcttc
    53041 ggcgacgcca acggccgcct ctccggaatt tcgccgtgtc tcaacacggg cttggcgcag
    53101 ccctggatgg ccgccgagac ggacctcgat ggccagccca gggtctgcgg ggcgtccgtg
    53161 gacatgggcg cgttcgagta ctcctccgcc gccatgcccg tcgggagcct gcgatgcgtc
    53221 atggagccgt cggccgttct cccgctgggc gcccgctggc gcgttcccga gggtccgcac
    53281 acgaactgga atgagagcgg tgaagtgctg cacaacctcg cgaccggcga atatacgatc
    53341 gtgttcagcg aggtggcggg ctggacgaaa cccgccgatg cgcacccggt ggtcgtcgag
    53401 ggcctggaaa ctcttgaaac cgggctgtac ttgtcgatgg cgccggacac cgggcccccg
    53461 gtgatcgtct ccgtcgagcc gcccagcgga ttcgtcacga ccaccaacca cttggacatg
    53521 ctcattaccg ttacggacaa cgtggccgtg gcccgggtga ccgtcaacaa gcacgacgcg
    53581 acgtgcgtgg gcggcgacca gtgggcctat accgtgcacg gggtccgcgg ggcctatcac
    53641 gtcgtcgaga tcgtggcgta cgacacggcc ggcaacgtgg cgtccgggga ggtggtgtat
    53701 gcgcgcgagc acgggattac gttgcatccc gtgtgggccg gatactggcg gatccggaat
    53761 ccgttctccg tgtcttacag ctaccgctgg gaagcggtcg agaccgcgga aaccggttcg
    53821 ggtgtgcttg tgccccacgg cgacacctac ttcacgacct cgccgcaagt cgagcacgtc
    53881 cagttgtacg tggacgaagt gctcatcgag accaaggagg ccagcgaggc ggaattcccc
    53941 gcgccgccct ccgatgagcg gttcatagac agtgacggcg acggcctgtt gaacatggaa
    54001 gaagatgcgg ccgataccga tcccttctct cccgacagcc atttcatggc ctccctgctg
    54061 tccccggacg gggcggctcc gcagccggag cgcctggcgc ggcaggctgt cggctcggac
    54121 ttcagcctgg cctggcccag cagcgccgac aacctctaca cggttgagat ctcgctcgat
    54181 ctgtgggact gggcggccgt ccccggctac accgacaagc cgggcacggg ttcgaccatg
    54241 agctacacca acgcctcggc cgcggcattg tttttccggc tgaagacaca gccggcagaa
    54301 cccgagccgt agtgtggcat gtcagggcgc gtagacttgg atccgactcg ccggcacgcg
    54361 gcgggagagg cgctccaggg agcggctgac ctgccggatg atctccgcct tgtcgagcgt
    54421 cagcagccgc cggtcgcgca tgatcacccg gccgttgatg atcacggtct gcacgtccga
    54481 cgcgcgcgcg gcgtagacga ggctcgccgg cacgctgtgg aggggctggt gatgcggtcc
    54541 gccgaggtcc acgaggatga ggtcggcgag ataacccggc tccaccgtgc cgatccggtc
    54601 cccgagcccg accgcccgcg cgctgccgcg cgtggcgatc tccagggact cggccagcgt
    54661 catgttctcg gccgcgccgg tggcttgctt ctgggtcatc gccatcaggc gcaggctctc
    54721 ccagacgtcc agcgtgttgt tgctcaccgc gccgtccgtg ccgagcccga cggtgacgcc
    54781 ggccttccgc atggcgacga ccggggcgat gcccatcgcg agcttgaggt aggtcttggg
    54841 gcagtgcgcg acggcggcgg ggcggtcggc cagcagccgg atgtcctcct ccgtcgcgcc
    54901 gacggcgtgg gccaggaggc tcgggaggcg gagcaggccg aggtcgtcca gcaatcggat
    54961 cggtgtgacg ccgcgtttct tcacgtgggc cgcggtctga tcgcgggttt cggccacatg
    55021 aatgtgaata ccgacgccga gttcgtccgc ccggtcgcgc gcggcgccca ggaagtcgtc
    55081 gtcgcacgtg tacggcgcgt gcggggccat ccaggtcgtg atccggccgt tcgccgcgcc
    55141 gttccagcgc cggcagaatt gcgcggtgcg gtcgagggcc tcgatcccct ggttgccgaa
    55201 cacggcccag ccgagcgccg cgcgggtgcc ggcctgctgc acggcctcag cggcgcggtc
    55261 catgaagaag tagtggtcgg ccacgcaggt gacgccggcc tcgatcatct ccgcgaggcc
    55321 gagcagcatg ccccagtaga cgtcctcctc ctgcaggttg ctctcgagcg gccacatgta
    55381 ctcgttgaac cacttctcca gcgagacgtc ttccgcgagc ccgcggaaga tcaccatcgg
    55441 cacgtgggaa tggctgttga tcaggcccgg catgagcagc atcccgcggg cgtcgaggac
    55501 ttcgtcggcc ggcgccgggt tgtcgcttgc gggctccacc gaggcgatac gcgcaccctc
    55561 gatccgtacg tcctgctcgt tcagcacgcg cgtctgcggc ccgggtagga tgcgcaacac
    55621 cttggcatgg cggatcaaca ggctcatggc ggcctcctga gttgtctgta ttctgcccga
    55681 gaccccatgg ccgtcaagcc ggtcgaacgg ccttgatcag caccgcgagt tcgtcgggat
    55741 ggaacggatt gtagggtgcc ggcgtgccgg agaagaccct ttgccatccc tcggcatccc
    55801 aaccgggcca ggtcgcggcg ccgcgacgga gatcctcgat cagcgtgggc agccccgccg
    55861 ctttccgccg ggcgtgcaag atgtggagga tcgtcggatg cagggaagcc ttctcgaagg
    55921 cggcgaccag gcgggggagg acgcgcggcc agtccaggcg ggccagggct tgcagcgcct
    55981 cggcctgctg gtccgattcc aggaaacgct ccacggcgcg gcaggcctcc tcgccgggat
    56041 agtccgccag cacccggatc agcgcgcggg agagcgggga cgtgcccgtt tccccgtcga
    56101 gccgcgccgc cagccagggg ccggccccgg ggatgtcgcc gccggcgcag agcaggacgt
    56161 tgaccagcga gggatccgct cggagccggt cgaggacggt ctgccggtac aggtcgtgca
    56221 gcccgtcgca aaggctgtcg acgaaacccc ggcgggcgct ctcaaattca ggggcctcgc
    56281 tgtagtagtg ggcctcgaca aatccgtcga tggccctgtt catgcgctgg cggtcggggt
    56341 tcacggcggg cattccgggc cgcggtcggg gagggcgcat cgtcccccgg gccggctcaa
    56401 cgggcaggcg caggcggcgc agcgggccgc gctctcgcgc cgccgcgcgg cgctccgcca
    56461 gcctgcccgc gccagggcga cggctgcccc cgcgacgatc atggccacca ccgcctgttc
    56521 ccacgtcatg cgtccccgcc ggttgttcag aggccgatcc ccaggagccg gccgccctgg
    56581 taaacgagcc ccgtgataac gtacgccagg atcgtcagac ttccagactg caggaccgcc
    56641 catttccagg agccgctctc ccggccgacg acggcgaagg tggccatgca gggcatggcg
    56701 atcagcatga agagcatgat gcagaacccc gtcagcggcg tgtagtggcg ggccagcgcc
    56761 tcgcggagcg gctcgacgtt ctcctcgtcc gagccgaccg agtacacgat gcccagttgc
    56821 gccacgaaga cctccttggc ggcaaaggcc ccgatcatcg ccgtgccgat ccgccagtcg
    56881 aagcccagcg gccgcaggac cggctcgatc gcctgcccga tgcggccggc cgccgtgtgg
    56941 gcgagcccct cggcctgtcg ggccgccccg gacaggccgt ccgggaagcg gtcgagcttc
    57001 gggtagctgg tcagggccca catgacgatc gagatgccga ggatcagcgt gccggccttc
    57061 cggatgtaaa ggcccccgcg catccacatg tgacgcagca tgccgcgcca cgtgggcatg
    57121 tggtacggcg gcagctccat gacgaacggc atggactcgc cccggaacac ggtggcgcgc
    57181 agcagccggg ccaggccgat ggccaggccg atgccgacga ggtagatgat ccacaggatc
    57241 ggcgcgcgcc agcgctccgg gaagaaggcg gggatgatca gcatgtagat cggcaggcgc
    57301 gccccgcagc tcatcagcgg caggaccagc atggtggtca gccggtcgcg ccgggtctcg
    57361 agcacgcggg tggccatgat ccccggcacc gtgcagccga acccgatcag catggggata
    57421 aaactcttgc cgtgcagtcc gatcttgtgc atcagccggt ccatgaggaa cgcggcgcgc
    57481 gccatgtagc ccgtatcctc gaggaaggcg atcgccagga agagcagcag gatgttgggc
    57541 aggaagacca gcacgccgcc gaccccggcg atgatgccgt ccaccagcag cgagcgcagc
    57601 gggctggccg acccggccgg ccagaatccg ttcacccggg cgccgagcgc ctccacgccc
    57661 cgctcgatcc aggccatggg cggctcgccc agcgtgaagg tcagcaggaa caccgcgtac
    57721 atcagcccca ggaagatcgg caggcccagt gcgcggtgcg tgacgatcga gtcgatcgtg
    57781 tcggacatcg tgtgccggat ctcggtcgtc gcgtggaccg cctcctggca ggcccccgag
    57841 atgaacccgt accggtggtc ggcgatgacc tgttcgggcg gcatgccgcg gatgcgctcg
    57901 atctcccggg cccggcggcg gacctcctcc aggatcgccg gctcgttcgc gagcttctgc
    57961 gcctcggcat cgttctcgag gagtttcagg gccagccacc gggcgttgta acggcgcgcg
    58021 aggtcggcgc gctccgccag ccggcgctcc agggcgtcca cggcgtcgtt cagttcggcg
    58081 tcgtactcga agcggcgcgg cgggcggcgg gcggggtggt cggcgaccgc ggcgatggac
    58141 tccagcaatt cgcggaggcc gcggccctgg tggccgacgg tctggatgat cggcacgccc
    58201 aggagttccg agagccgcgc caggtcgaac tcgatgcctc gcgcgcgggc gcggtcgctc
    58261 atgttgaacg ccagcaccag cggcgcgccg agttccatga gctgcacggc gaggtacagg
    58321 ttgcgctcca ggttcgacgc gtccaccacg tccacgacca cgtcgggccg ctcctcgatc
    58381 aggaaatggc gggcggcgag ttcctcgggg gagtaggccg tcaggctgta cgcccccggc
    58441 aggtccacga aggtgatctc gcggacgccg tggcggcagc ggccctcccg tttctccacc
    58501 gtcacgcccg ggtaattgcc cacgtgctgg cgcgcgccgg tcagcgcgtt gaacaggctg
    58561 gtcttgccgg aattcggatt gccggcgagg gcgacgacca gggggcggct cacggcggca
    58621 tcctctccac ctgcaccccg gcggcctcgg ccttgcggag cgagagccgg tagccgcgga
    58681 cctggacctc cagcgggtcc cccagcgggg cgatccgctt gacctcgacc accgagccgg
    58741 ggatcagccc catgtcggcc atccgctggc ccagcgcgcc gcggcggtcc agccggacga
    58801 tccggccgcg ctcgccgggc ttcaggtctt tcagggtatg gcccgcgctc atttccgaga
    58861 tgtccttcgc ggtcgccggg cggccggcgc cgggcagtgg aagccgcctc cgttgatcca
    58921 gcgcgggccg ccgagggggc agcgtttcat cagccgggcg aactccgtca accgccgccg
    58981 gatggcgggc gagagggcgt gctccatctt gcaggcgacc tgctcggcct cccgctcctc
    59041 cacttcgagc accttgacga agaaccggcg cagcgcctcg tgccgccgcg agatgtcgcg
    59101 cgcggcggtc cgtcccttcg cggtcagcgt gacgcggtca tagggggcgt aattcacgag
    59161 gccccgggcc gagagggcgc ggagggccag cgtgacggac gggcggcgca cctggagacg
    59221 ctccgcgatg tcgcgtcccc gcgcggacgc cccgtgggcg gtcaggaggt ggatggcctc
    59281 caggtagtcc tccaggctcg agctcaaggc cggagccata ttctttggcg gtgcggtgcc
    59341 cataaaatgt tttgtgcgcc taactttagc ttccaccccg cccttgtcaa tcccgttccg
    59401 aagtcttgca ttcggggccg cggggcggca cgatggccgc gcgatggccg attcgaggga
    59461 caccctgtcg ctggacaaca tccgcaacgt cctggtgcgc ctggaggaga cgatcctctt
    59521 cggcgtcctc gagcgcgcgc agttccggca gaacgccgtg atctaccggc cggacggggt
    59581 cgggcggggc ctgggcgggg aaagcctggt gcagttcctg ctccacgagt gcgagcgctc
    59641 gcacgccaag gtccgccgct acaccagccc ggacgagcac ccgttcttcc atgacctgcc
    59701 cgcgccgatc ctgccggccg ccgcctacga caatccgctc cggccgaaca ccctcaacat
    59761 caacgaccgg atctgggcgg cctacgtcgg cgagatcatc ccgttcctgt gcgcgcccgg
    59821 cgacgacggg caatggggat cgagctcggt caacgacgtc catctgctcc aggcgctgtc
    59881 caagcgcatc cactacggca agttcgtcgc cgaaagcaag cgccgcgcga ggcccggcgc
    59941 cttcgatccc ttgatccggg ccggcgactc ggacggcctc cgggcggtca tcaccaacgc
    60001 ggacgtggag aggggcgtgc tggagcgtgt agagcacaag gcgtcgacct acagccgcga
    60061 aatcgaggcc atccccgggc agctcgcggc aagcgcggcc tcggtccggg aactgtacgc
    60121 gcgctggatc atccccatga acaaggatgt gcaggtgctc tacctgttgc agccgggatg
    60181 aagatggggt ggatgacggg atttgaaccc gcgactccca gggccacaac ctggtgctct
    60241 gccaactgag ctacatccac cgccggaaac acagcatata cgggcgggcg cgtttttcca
    60301 gacgaaaatc cggcgaccgc tcgctccgtt ttgctgccga cggcctaccc gtttcgcggg
    60361 gcgcggcccg gcctgttttt tcttgccgtg atctgttgac gccgggcgat aattacgtga
    60421 taaacttccg caatcgataa ttattacgta gaaactgtta tgcgcgtgct ttttctatac
    60481 ccggttccgc ccgcccgctg gcagcggctt cgctaccagc agggcatcgg ctcgatttgc
    60541 gcggtgctga agcaggcagg gcacgaggtg gagctcctgg tgctttcgga accggaccgg
    60601 gaccggatcg cggaggcggt ccggcgattc aatccgggcc tggccgcgat ttcgctgacc
    60661 agcgcggagt tcgatgtggc ctgcgccatg ggcggcatcc tcggccgcga gcatcgcctg
    60721 cccgtgatcc tcggcggcat ccatcccacg ctccgccccg aggagtccat cgcggcggac
    60781 ggggtgttcg caatttgcat cggggagggg gaatacccga tactcgaact gtgcgaagcc
    60841 ctggagcgcg gcgcggaccc ggccggcatc cgcaacctct gggtgcggcg gaacgggacc
    60901 atccaccgca acgaactgcg cccgctgctc tcgcggctcg acgatctgcc gtttcccgac
    60961 cggaaggtct ttcgttttga cgacctggtc cgcgattttc cggaggcgga gttcatgggc
    61021 agccgcggat gcccgtaccc gtgcgcctac tgcgctaacc attccctcct tcaactctat
    61081 cgcggccacg gcccctatgt ccgctaccgg agcgtggacc gtctgctgga tgaaatggac
    61141 gctgtggtcc ggcactatcc cggcatcgag tggctcggct ttcacgatga cactttcacg
    61201 atgagccgcc gctggctatc cgagttctgc gagaagtacc cggcccggtt ccaacttccg
    61261 ttctggtgca attcgacggc caccctgttg aacgaggaca ccgtcgcctg cctgaagaag
    61321 gccggatgct acgaggtgcg tgtcggggtg gagtccggca acgaccgcct ccgcatggac
    61381 gtcctgcgca agaaggtccg ccgggaggac atcgtgcgcg ccttccgcct gctgcgggag
    61441 gccggtatcc agggctattc gttcaacatg atcggcctgc cgcacgaaac cccggggacg
    61501 atccgcgaca cgatccggct gaaccaggaa atccggccgt ccacgacgtt ctgctcggtc
    61561 ttctacccgt tcccgggcac gcaggcgcac gacgtatgcc gggcgaacgg ctggctcacc
    61621 gggcgccggg tcgcgagcta tttcgagaac gactacgcgg tgaaccagcc gaccgtcacg
    61681 cggaaacagg tcctgttcta ccacgcgatc ttcggcgacc tggtccggtg gccccggctg
    61741 gcgccgctga tcacgctggc gcaccgggtc cccgtgtggc cgggcaagtc gctgtggaac
    61801 gcgatccggc gagttcgcgc gaaggtcgtg gaatggggcc ggcatctcgc cgggtgcttc
    61861 cgcccgtgtc cggttggcgc cgaggtggac catgcttaag cgcgcggggc ttgtcctggc
    61921 cgggttgctg gtcgccctgg cgttcggcga gtgggttgca cgctggtcgg gccgcgcgcc
    61981 cgagatgacc ctgatcaccg ccgggcgatt ccggctttcc ccgaatcccg cgatcggcta
    62041 cgagccggcg ccgaacttcg agcaccacgg cgagaagcgg ctctatttcg agttccaggg
    62101 ccgcagcaac aacctcgggt tccgcgaccg cgaccacacc ttggagaagg agcccgcagt
    62161 cgtccgcctc gtcgtgctcg gggacagcct gaccatgggc ctgttcgtgg accggaccga
    62221 tgccgtgttc acctccgtcc tggagcagtc cctggccggg cgcgggaagc gggtcgaagt
    62281 gatgaatttc ggcgtcagcg gctacaacac ccggcaggaa gtggccatgc tgatcgagaa
    62341 gggcctgttc tacaggccgg atgtcgtggt cctcgcctac tgcctgaacg acgtggagga
    62401 gatgaacggc ggcatcatga ccaagctgcg cgtggagaag aaggagcacg ggggtatcga
    62461 gcggagcatg ctgtcgccgt ggctggcccg cagcgccctg tatcgcgtgg tgtacagcca
    62521 gtggctgtcg gcgtccgccg agaaggaacg gaaagagcag cgtatgctgc tgaaacagga
    62581 taccgtcgag tccagcctcc ggctcctgcg cgaaacggcg gagcggaaca agttccgggt
    62641 cctggtggcc gcgttccccc cgcgggagaa ccgccgctcg ccggagcggt tgcggcgcat
    62701 caccgacctc tgcgcatcga acggattcga atacctcgat ctgcagccga tattcgatga
    62761 gcgggccgcg aggaccggtg aaaagctcat gcacgacggc tggcatccca cgaccgccgg
    62821 gcaccgatgc gcaggcgagg ctatcgcgga cggcctggtc cgccgcggcc tggtgccctg
    62881 aagtccgctg gcggcgggtc acgggcccgg ccgccggatg gcgatggatt cgatccgcat
    62941 gtccgcgttg cggaaataca cgaacaccag gttgaccgga aggttgtcga gctggtccca
    63001 ttccatgcgg gcggaagcgc gcccggccac gacgggggtg gacgtcgcat cgtcccgccc
    63061 ttccacgtac agccggccca gttcggcgcc cgccggggcc ggagacgcga aggtcagctc
    63121 ggcggcgtag cggcccggcg cgaggggcag cttgggcccg tagaggatgg cggcgtacgg
    63181 gtccgatgac ttccgcagcg cgacgacgcc gtttcccgcc ggctccgtgt agccgccgtg
    63241 gaagaagcac ggggcggcga tggtccggct ttccccgggc gccaggtccc gccaatcgcc
    63301 ggccgcgagg agcgccaggt ccgcgtcgac ggtcccttcg gcccggtcca gccgcagcgc
    63361 cagcgaggag aagtccgcaa ggcttatcgg tgcttcgtgc caggtccacg cgtcggcggc
    63421 gaccgggatg tcgtgccggc ccgcgggctg gccgttgacg agcgtggtgg cgccgagcag
    63481 gcccgggccg cgcgcgcgga tcatccagcg caggccgggg atagggcttg ccatggtcga
    63541 tggcgtttcg acccgggcgc cggcttgcgc cagggccagg ccgccgcccc ttgaggccgc
    63601 gagatcctcg accgcgcggc agagcgtgtt cgagcatcgt tccagttccc agcgccgggc
    63661 cgggaagacc gcggggccgt ccggcagccc gggctcgccg gccgcggctt cgcgaggcgc
    63721 gggccggatg cggaacgccc agaccgtccg ctcgtgggcc aggaaatcca gccgcgggtg
    63781 ccggcgcagg cggttcagcg tggttgtgac cgggaagggg ctgacctgct cggggaacag
    63841 gttctcgtgc agcagcagat accggacgcc ccggcgcagg aggtcatcca gcgcggcgtc
    63901 cggcgcccag ccctggttga ggctgcggag cgcgccatac gcgtcgcgga cgtacgcccg
    63961 ccgggggacg gggctgtagc cgttgaccat ccggatgccg tgcttctcgg caaagtactc
    64021 gtagacggag ctgaaggcgt cctgtcccgg ccagagcggc agcgccaggg cgcgcggcag
    64081 ctcgtcggcc gcccgcgcgt cctccgccac ggccgcgtag gccttctgct cgccggccag
    64141 gacgcacagg gtcggccgca cctgggcccg ccactcgaag accaggccgg ccaccagcag
    64201 cccgaaggcg gccgcgaagc tctttggccg aagcttccgg ccggcgatct ccgcggccag
    64261 ccccgcggcg acggccagga gaacgggcat gaggcagtag accttggcgg tctgccgcag
    64321 cagggagtac ggcggcacca ggagccggat gatcaccagc aggcggcccc cgagaggccc
    64381 ctggacgccg agcgacaaca atacggcgaa caccgtcgcg gcgcccagca gcaagaacgc
    64441 cacggctgcc cgccgctcgc ccggcgtttt ccatgcgctc cgccgcagca atccggccag
    64501 cccggcgagc aggagcaggg gcagcaggag gccgacatag atgtggctgt ccaggccgga
    64561 agacgcccag cgcaccaggc ctgccggcga cggggagaag ttgctgacct cggccatcgt
    64621 gcggggcgcg gccaggtcgg cggcggagag atgatgctgg cgcatccagc ggtagaggac
    64681 gatcagggcg acgaagaggg cgatgggcgc cagcgcgcgg aggatccggc gtcgccaggc
    64741 cggctcgcgc cacgcggccg gcccggccgg gagcgcggca agcaggagcc acagcggggt
    64801 ggccatgccg cagaagaaca gcgactggat gtcggtccag cagagcatca gcagcgccgc
    64861 gcctgccagg gccccgcccg cgatgcgccc gtcccgcgcg gcgaggtcga gccccagcca
    64921 cacggccggc acccataaca tggcaaagcc cgtggggctg ccgcccagca ggcataccca
    64981 gcggtagggg agcaggatgc cgatgggcgc cgccagcgcg gcggtgacct cggagcggac
    65041 gaagcggcgc gccagcgtcc acgtgagcca gtaggtcagc cacaggctga cgaagcccgc
    65101 caggttccac gcgaaggcct ggcccgcgag gcccgcgaag gcggcgtaga gcagggagaa
    65161 gggggcgtag taggcgatgg gaaggaagcg gtcctcgtcc cggcccaggt tgaactcgta
    65221 cacgttgtgg aaccacggcg tgcggcccgc cagcatcccg tgcaggagct cgaaatggta
    65281 cagcagttga aggtgatcgc ccgggatcat ctctcgcgcg tggtcctgct cgacgttctg
    65341 ggacgaggcg ggaatggcct cgaacaggtg acggggcaac ggccaggaga agagcgtcca
    65401 gaacgccagg ctggcggcga gggaaatcag gagccggcgg ccctgcgtca cggcatcccc
    65461 cgggccttcc ggttccgcag gagccggagc gccacctgcg acgggcagag ccgcgcgccg
    65521 gcgcgttccc gcaacggcgt atcctggagg agccgccgcg cgatcgcggc caccgggcgc
    65581 gcctcgtcca tcggcgccgg cgcgtacacg gtggccaccc gtcccgccca ccgggcgtcc
    65641 tcggcgaagg gcaggggatc ggaaccggac acgacgcgga gcccgcgccg gcggccggcg
    65701 cgcatgagcc ggggttcgcc ccagaaaagc gggcgcaagc tggtgtcgcc caggagaaga
    65761 tcgcccggcg ccgaccgcga gatgaacgcg ccgacgatcc ggccccgccc gaagaaccac
    65821 ttgcccggcg cccagctcaa cacgggcagg ccgccctgcg cgcggacgcg ggcgacggta
    65881 tcctccagcg gcaggccgtc ctccacgaac gacgccatgg ccaggcccag cacctcgatc
    65941 cgctcccgcg tgatgacctg gcggccggcc aggagatacg cgcacccctc gcccgacccg
    66001 ccgtcaattc gcagcccggc cggctcgtcc ggcagggcgg acacctttcc ggggtcgagg
    66061 cgcagtcggc cggatttcaa ctcgtcgaag acatggtgat cgtgccgttc ggtgaggcac
    66121 aggaccggca gggcgtcggg ctgttgcgcg gcccgggcca gctcccgcag gtgttcgatc
    66181 gccttccgga tcgtgtcggg gatgtcgtag cagcggtaca gatgaacatg tgtatccgcg
    66241 atcaacccct tgcctgtacc tgcttcctgc atacgatagg cctataccgg aagcccccgg
    66301 ccggttcaag aaagcccttc gattaaaccg cgccattcca tgtatcgggc gaatcgggtc
    66361 cgcctctttt taatcttgat cggacgggta attatgttgt aaatgtcaac acaaagatgt
    66421 tgctacgtag taacttatcg aatttatctc catgaatgtg ttgtttctgg cgccgcagtc
    66481 cttctatctc gatcggggca ccccgatcgc ggtgcgcctg ctcctggagc agttcagcag
    66541 gcgcggatgg agcgtggatg tcctggtctt cccgggcggg agagacgtcg tgatcccggg
    66601 ggttcgtttc ttcaagacct cgttgcgccc cggcgcgcgc ccgcttcaac cgggcctttc
    66661 cgcgcggaaa ctgtggcacg acgtgcggct tttcttcgcc gcccgggaac gcctgaagac
    66721 gcgacgctac gattttatcc atgccgtgga agagggcgct ttcatcgcgg ccctcttgag
    66781 cgcgcggacc ggcgttccat tcgtctgtga catggattct tcgatgcccg accagttggc
    66841 ggaaaaaaac cggttctggc gcctggtgcg gcctgtgatg cgccggttcg aagcgtacat
    66901 gctgcgccgc gcccgcgcgg tgctggcggt ctgcgaggcg gtggccgaca aagcccgggc
    66961 cgtggcgcgc ggcccggtgt tcgtgctgcc cgatccgccg gtcgggggcg ctccgaaccc
    67021 ggcccggaat catgtgcgcg aacggctgaa ggtggagggc ccagcctttg tatatgtggg
    67081 caaccttcag ggctaccagg gcatttccct cctgttgaag agtttccagc gggcgctggc
    67141 gcatatggcc caggcctgcc tgatcatcgt gggcggcgaa ctgatggaag tggagaaact
    67201 cgagcgccgg tgcaaccgcc acgggttcgc cgggcatgtc catttcacgg gcgcctgcgc
    67261 cctggacgag accgtccagt acctggatgc ggcggacgtg ctcatctcgc cgcgcatccg
    67321 gggcgccaac accccgatga aaatctacag ctacatgcaa tccggcaagg ccatcctcgc
    67381 cacggacatc ccgtcgcata cccaggtgct gacgcccgaa gccgccgtgc tcgtgccccc
    67441 gcagccggag gcgatggcgg aggccatggt caagctggct tctgacgccg acctacggga
    67501 gcgcctgggc caggccgccc gccgcctggc ggagacgcaa cactcgcgcg cggcctacga
    67561 gcggaaagcc gacgagttct gcgcgtggat ggaatcgaac gtggcgtgag ccggtgaagg
    67621 tcaggatgcc gccggtcggc ccgcgccccg cagccagccc gccccgacat accattccgt
    67681 cgtctgccgc aggccggcct ccgacgagta gcggtactgg aagcccagct tctcgcggat
    67741 cttgcgcgtg tcgaaggagc ggtccttggt gtagaacgcc acgcgccgcc ggtagagggg
    67801 cggctcgatg ccgaacggac ggcaggccag ctcgcagagg tcggcgagca cgaacaccgg
    67861 cccgaccggc aggcggatca cgcggagccg cctgcccagc gcggccgcga tcgcccgccc
    67921 catgtcctcg aggcggatcg cctccgggtt tccgcagatg aacacctcgc ccgccgcggc
    67981 gggatggacc gcggccagcc gcagcccggc ggccaggtcc tcgacgtgga tcaggtggta
    68041 caggcatttc ccgcggccca gcagcgggaa ccagccccgg gcggccatct tgaacagctt
    68101 gaacaaccgc cggtcgcccg gcccgtaaat ggccgcgggc cggatgacgg agaaaggcag
    68161 gccctccttt gccgcaaaat cgcgcagcca gagctccgcc tccgccttgg tcttctggta
    68221 gatgtcgccc gggtggaagg gatagttttc gtccgctggc gggttctcga tgtggccgtg
    68281 cacgccgatg gtggagacgt ggacgaaccg ccggaatccg ggccggcccc ggaccgcgcg
    68341 ggccagctcc tgcgtgctct gcacgtgcac cttccagtac tgctcgtcgg ggtgccgcgc
    68401 ctcgcggtac aacgcggcca cgtgaaaaac atactcgacg tccgcgaccg cctcgcggat
    68461 caccgcgggc tcgaagacgt cgccgcggaa ccagcggacc ggcaggtcgg ccagcggggc
    68521 gaggttcgag gaggcgcgtg cgatggcgcg gatgtccgcc ccggcctcgg ccaactggcg
    68581 ggtgagcgcc tggccggtga acccggtcgc cccggtcacg agaacccggg cgccgcgcgg
    68641 cagggactgg atcgactcgg tcatcatcat tcctttcgct ccgcgcggag caatgtcggc
    68701 gacccgaaga gcgccgtcaa tcccaatccg cgcgccgggc tttccagcgc ggcggacagc
    68761 cgcggccggt cgagcatccg gtgcagcacc atgggccaga agaactgcgc gcggcggccg
    68821 gcgaccgcga agcccgagcg ctcgagttcc cgcgcgattt cgtcgtggcc gaacagcgtg
    68881 aacggccggg tgttcccctc cacgcggcgc ttgaggccga acaggaggcc cgagagcgcg
    68941 ttgacgctgc ggcgggcggg atagtccacg attaccgcgc ggcgcgcgac gcggcacaac
    69001 tcgcggacca gcgccggcca ggcccggcag tgaggcagga gccgtatgct gaccgcgacg
    69061 tcgaaggccc ggtccgggaa cggcaacgcc acgaccgacc cggtgacgaa ggccagccgg
    69121 ccctccgtca cctgccggtc gatcatcgag caacaactcc cgtcgctgcc gagcaccgtc
    69181 acgcggtatc cgttccccgc cagcgggagc gcgagctgcc cgtggccgcc gcccacgtcg
    69241 agcacggtcg cgccgggaaa atccgccatc cagccgagga caatccgctc ctgcacgccc
    69301 agcatccatc ggccggcggg cccggcgaaa cgggcagcat agccgggcga ggaggtctcg
    69361 acgtcggcgg tctccgggat atccggcaag gtggtgtcgc gctcgttcac ggcccgccag
    69421 tgtagcaggt cgcggatgac aggccagaaa aacgggtggc gccaagcatt tccattgccc
    69481 cgccgcggcg cggccgctag actccatcag actgcgcggg aatcatcagc ccaacgtgaa
    69541 agtactgttc atagcccccc agccgttctt cgtggagcgc ggcacgccca tccgcgcgcg
    69601 ccaccagatc gaggtgttga gccggatggg gcaccgcgtg gacgtgctct gctacccgtt
    69661 cgggcagccg gtggacctgc ccggggtccg ggtgctccgg acgttccggc cgcccggcct
    69721 acgccaggtc gccatcggcc cctcgctggc gaagttcccg atggatttcc tgctgctctg
    69781 caaggcgttc gggctgtgcc tgtggaaccg gtacgacgtg atccaggcgg tggaggaggc
    69841 ggcgtttttt gccgtgccgc tgaagaagct gttccggtgc cggctgatct acaacatgga
    69901 ttcgttcatc tcggaccagc tgcaattttc cggcttcgtc cgggcccggc ccgtgatccg
    69961 cctggtcgaa ggactcgagc gcgcggccat gcggaacgct tcctatgtgg tgaccgtggg
    70021 gccggtgctc tcggacgtcg tgcggcgatg ggccccgcga acgaaggtgc tgcaactgga
    70081 ggacgcgccg ctggaggcgt ccttccggga ggcgccggag gacgcgcggc ggatccggga
    70141 ggagttcgcg ctgggcgccg cgcccgtgtg cgtctacacc ggcaacttcg agcggtacca
    70201 gggcgtggcc gacctcgtgc gcgccgcggg cctggtcgcc cggcagcggc cggaggcccg
    70261 gttcctgttc gccggcggca cggaagccca ggtggcggag atgcgccggc tcgccgacga
    70321 ggccggggcg gggtcggcct gcgtattcgc cgggcggcgg ccgaccgccg agatgaccgc
    70381 cttcctgaac gtcgccgacc tgctcgcgac gccgcgcaac cagggcacga acccgccgat
    70441 gaaaatctac ggctacatgc agagcggccg gcccatcgtc tcgacccgcg tccccaccca
    70501 tacgcaggtg ctcgacgact cgtgcgcgtt cctggccgac cctacgcccg agggactcgc
    70561 cggcggcatc ctggccgcgc tgggcgaccc ggaccgggcc cgcgccgtgg cgcgggccgc
    70621 ccgcgcacgg gtggagcagc gctacagcct ggagatcttc cgggagaaag tgcgcgcggc
    70681 ctacgcgggg ctcgaggcgg aaatccgcgc cggccggggc gcggcgtgat cagccgccct
    70741 tgtcctcgcg ccggtagggt ttgagcaggc cggccggggc ggacgccttc cgggacacgc
    70801 tgatcatggc cagcacggtc atgatgtagg ggatgatcag gaagacgtgg aagggcacgt
    70861 ggaggcccac ggcctgcagg cggagctgga ggccgtcgag cagcccgaac agcagcgcgc
    70921 cgagcgcgcc cttggccggg ctccagttgc cgaagatcac catcgcgatc gcgatccagc
    70981 cccggccccc gacgacgtcc atcaggaaca tgttctggtg ggccagcgtg agaaaggccc
    71041 cgccgatccc catcagcgcg ccgccgatca ccaggctgac ggtgcgcgtg cggtggacgt
    71101 tgatgccgac cgtgtccgcc gccagtggat tctcgccgac cgtgcggatc cgcaggcccg
    71161 cgcgagtccg gaacagcagg atcgagaccg ccgggatcag cagccacgag aggtaaacca
    71221 gcgcgtactg gtggaacagg gccggcccga ggacggggag ccgcgcaagg agcgggacgg
    71281 ccaccggccg gaagggctcc acggtcgggg gcaggacggg cgagccgaac acgagccggt
    71341 aaatgaacat ggcgaggccg gtggcgaaga gcgtcagccc cagccccgag acgtgctggc
    71401 tcaagcccag gtagacgacc agcacggcca tgatcagccc gaggagcatc ccgacgccgg
    71461 ccgccgccgc gacgccgagc cacaggtggc cggtcttgaa ggccaccgtg aagcccgtca
    71521 tcgcgcccat cagcatcgtg ccctcgatgc cgaggttcag cacgccggcg cgctcgccga
    71581 cgatctcgcc gagcgtggcg aacaggatcg gcgtcgccat ccgcaacgcg gcggcgagga
    71641 cgcccgtcag gaaggcgagg ctcaagaggt catccatggc cgcctcccgt ccccgcgccg
    71701 cgccggaccc gcacgcggta ttccgtcagc agcagggcca ccagcatgac gaccagggcc
    71761 aggccctgga tcacctcgga gatgtagctg gggacgcccg tcatgcggct catggtctgc
    71821 gagccgacgc tgaccgcgct gaagaagagc gccgcgagca cgacgccggc cgggtgcagg
    71881 ttgccgagca tcgcgacgac gatccccgag tagccgtagc ccggcgagag atccatcagg
    71941 aggtggtgct gcacggcgca gacctcgccg acgcccgcca tcgcggcgag cccgcccgac
    72001 aggaccgccg tcttgagcag cacggacgac gtcggcacgc cgccgaagtg cgccgcgacg
    72061 gggttggcgc cgacggcccg gctttcgtag ccgagcaccg tcttgcggtg gatgagccag
    72121 accaggcccg ccgcgacgag cgccagcggg atgcccaggt ggaagcgcga gcgggcgacc
    72181 aggaccgggt actgggccgt ggcccggatc tccggcgatt gcggccagct ggagtccggc
    72241 tgctggagcg ggccgaagag cagcgcgccc atgacgtgca tcatgatgta attgaacagc
    72301 agcgtggtga ccacgtcgtc cacccgcagg cggaatttca gccaggccgg gatcgccgcc
    72361 cacaacccgc cggcgaggaa cgcggcgccg atgatcacgg ccaggtgcag gagccgcgag
    72421 gggatcggca gcgcgatgcc cagccagccg gcgacgatgg cgccggccat cagttggccc
    72481 tccgcgccga tgttccagaa gcgcgcccgg aacgcaaacg cgacgccgag cccggtgaag
    72541 atcagcgggc tggccttgac gcagctctcc agcaggttga cgcgcgaccc gagcgcgccg
    72601 tagaagaggt agtagtagga gcgccacagg ttcccgccgg cggcgaggat cggcgccgcc
    72661 gccagcaaca gcgtggccgc gacggccgcc aggggaatgg ccgccttcgc ccagtcgggc
    72721 agcggcggac gtttctccag gcgcagccgg atcatggaac caccccgctc atccagagcc
    72781 cgatctgctc ccgggtgatt tcgttcacgg cccggatccc gacgacccgg ccttcgtaca
    72841 tcaccgccac gcggtcgctg acggagagga tctcgtccag gtcctccgag atcagcagga
    72901 tcgccgcgcc gcgcccgcac tcctcgagca tccggccgcg gatgaactcc gccgcgccga
    72961 cgtccagccc gcgcgtcggc tgcgccgcca cgatcaggcg cggcccggcg gacaattcgc
    73021 gcgcgaggat cactttctgc aggttgccgc cggagaggct tttcgccgcc gtcgacggcc
    73081 cggccgcctt gatgccgaac tggccgatca gccgtccggc gtgctcgcgg atccggccgt
    73141 ggtccagcag gccgcggcgg cggaactccg ccgagcgccg gttttccagg atcaggtttt
    73201 ccgcgatcga caggtccatc atcaagcccg tcgcgatacg gtcttccggg atgcgcgccg
    73261 ctcccgcggc gatgacggcc gtcgggctgc cgggccgggt cggggcgccg ccgatgcgga
    73321 ccgagccggc cgtcggcttc cgtacgccga agagcacctc ggccagctcg cgctgcccgt
    73381 tgccggagac gccggccagt ccgaggacct cgccctcgcg gaccgccagg tccaggttcc
    73441 ggaccgccgg caggcccttg tcgttcgtcg cggacagtcc gacgatctcg agcgcgggcc
    73501 ggccgggcgc ccgcggcgag cgctccttcc gggccggcca atcccgcccg accatcaggc
    73561 cggccaattc ccgcgcattc gtgtcgcgag tttgtttctc ggccgtgacc tcgccgttac
    73621 gcagcacgac gatgcggtcc gagatgtcca tcacctcgtg caacttgtgc gagatgaaca
    73681 ccacgccgtt cccctgcgcc gcgaaggtcc ggagcatggc gaagagttcc cggacctcgg
    73741 acggcaccag cacggcggtc ggctcgtcca tgatcaggac acggcactcg cggtagagcg
    73801 ccttgaggat ctcgaccttc tgctgctcgc cgaccgagag ggtccagacc ttcgcgtcgg
    73861 gatcgagcgt catgccgaag cgccgctcca gttccagcag ccgcttccgc gccgccttcc
    73921 agtccaggaa cccgaggcgg tgatcctcct gcccgacggc gacgttctcc aggaccgaca
    73981 gggacggcac gagcgcgaag tgctggtgga tcatccggac gccgcgccgg acggcggccc
    74041 ggggcgagga gaggttcacc cgttcacccc cgatccggat ctcgccctcg tcgcaggcgt
    74101 agtagccgaa gaggatgttc atcagcgtgg ttttgccggc gccgttttcg ccgagcaggg
    74161 cgcatatctc gccgggctcc aggcggaagt ccacgccgcg gttggccgga acgccgagga
    74221 atcgcttcgt gattccccgc atctcgatca ggggcgtcgg gtcggtgttc atgccgcgcc
    74281 ggttatttat ccggaacggg ggcaaacctc aatcctttcc cgccctgctg cataaatcgc
    74341 ttgaaacgtt tcagcctccc gagtaagttg gcgtacgaaa ggagccatca tgaaaacccg
    74401 cggcgccccg ttctgccttc tatctgtcct gggcctgttg acgctatcgg ccttcggcgg
    74461 cacgacgccc cagaaagtgt tcatgctcat ggataccgag gcctaccagg cgctcaccaa
    74521 cgaggtcaac cagtggaagg ccgatgtcaa cgccgagggc cggtaccagg ccgaggccta
    74581 tgccattcca ccggacaacc cggcgctgct gcgcctgttg ctggcgctcc actaccaggg
    74641 cgggaacctc gctggcgtga tcctcgccgg ccgcgtgccg tacgtcatgt gcgagcagga
    74701 ctccgagtac ctggtcacgc cggatgcctt tcccgtggac acctatttca ccgatttcga
    74761 cgccgagtgg tacgatctga acctgaacgg aatcatggat tcgtactatg ccttcggcgg
    74821 gttccagccg gacatctggg cggggcggct tttcacggac acgatcaagt tcaacagcaa
    74881 gacgcggatc gacctgctgc gcgcgtattt ccagaaaaac caccaatacc gcgccggtac
    74941 gctgaccttg cccaagtcgg cgctggtgta ttacgacatc aagaactaca ccacggccaa
    75001 cgatttcggg aaactctatc ccaccttcca gaccgtatgg ccggacacca gcagcggcac
    75061 ggactacctg aaccggctgc tgcccctgcc gggcaacgag ctggtctatc tcgactgcca
    75121 ttcctcggcg aagacgcacc aacttacgcc cacgtggatt gcgtccgacc tgatccacgc
    75181 cgtcaatccc aaggccttct ttctcctgct cgacacctgc cagagcggcc atttcacgga
    75241 gacgaactac ctcgcgggca gctacctgtt cgacacgaaa tacccgctct tcgccatggc
    75301 cgcgaccacc gggtcgggat acaacttccc caccggattt cagaccctgt cggcttgggc
    75361 gggcgaggac tgggggagca tcttcatgca ctttgcgagg gagcagagct ggtcgcgcga
    75421 gtacattctc ctgggcgatc ccacgctcat cccgcacctg aacccgcccc aggttgcctt
    75481 gtcgctgccg tatgccttct cctcggccgc gcccctgaag atcccgcgtc cggcctccgg
    75541 ggccgcgcag gagtggcgca tcccgatccg cggcaagctg accgtcgggg cggggaactg
    75601 gctgcgggtc actgcggcca acgcgccggc cggtatggcg ccgcggacga atgatttcca
    75661 cctgaccgtc atggggttga ccaatgcggg cgacgcgacg gtggccgtga cgccgacgac
    75721 gacgctgggt aagcggcagc ttacggtcgt ggccagggac agccgcggga agacggccgc
    75781 cctgccgtac tggatcgaag tggtcgcccc gccggcgacc gggaagccgg acttcacctt
    75841 cacggtgggc gacacgaatc tcgtattccg atacggcgag ggcgatccct tcggccgcct
    75901 gacggtccag gcccagttga ccacggcgga cctgtccgcg ccgatgctgg tcaacctggt
    75961 gccggtgaac tggcccgacg ggttcagcct ggacaaccag acgtcgtccc cggaatcatg
    76021 gagcggcggc ttcctcctgc agcagaaggg cgttacgcgc tacacgtatg tcaccgttcg
    76081 catgcgcagg gacatggcgc ccggccagta cgttctgccg ctcaaggccc gcgccatggg
    76141 cgtggagaag acggtgaacc tgacggccgc ggtccttccg ccaagccttg aggtgacgac
    76201 cgtgccgtcg cccgtgtata tcctgcccgg cgacgaggcg agcatcttct cgaccgttgc
    76261 aatgaacggc aacgcgcggg tctacttcga cctgttgtcc taccccggga tcgaggtgca
    76321 aaaagtgctg acgaccatgc ccttcgttcc ctacgggggg cactcggttc acgtgtggac
    76381 cgattgggac gccgcgtccg gggcctattc cctcctccgg taccagttcc ccgatatgcc
    76441 cggcgctccc gtcggctacg ggctggacgt ggtcgtccgg ccgcaattca ccctggacat
    76501 cgaactcgtc tccggcggtt ccgggggcgg tgtgctgcag gtgacgatgc acaaccaggc
    76561 cgtcggcaac ccggcatacc cgctgaccgt caacgccgaa agcgacgcgg tggcggaagt
    76621 gaatgtgctg aatccgcagg tcacgatgcc ggccggcttc ggcgcggaag cccatgcgct
    76681 gatcgagttc agcaccccgg tggcgctgct gccgccgggc ggccactcga tctactacac
    76741 cattaccatc cgcgtgacct cgccgatgca ggagtggcct gaacaggaat gtgcgctgac
    76801 cctgaagctg atggcgccgc cggcggctcc gacggagtgg gaaatccagc ccgtgcattt
    76861 tatccccgga cctgatccgg tgccctgcga gctggacctg ccggtgctga tctacggcaa
    76921 ccagcgtttc acggccacgc cgcttgtcgc cacacggttg cggtaaagga ccgccgtcaa
    76981 tccgagaccg tcttcgattc atccaccggc acgcggaacg cgccctggac gatcttcgct
    77041 tccaggtcgc ggaccgacgc gaggacgtcc gccgggagcc gggcctcgaa atcgtgcaac
    77101 ggggccagcc tggcgccgcc ctgcgccatc atggaccagt ccttgaggtc catgccgacc
    77161 cacgcgccct ttcgcacggt ctcgatgcag tgccggatgg tggggcccat gtcccatacc
    77221 ggcccggtga tgatcgtctc cggcgccatg ccgtgctggt cgtacatgtt gctgaaggcg
    77281 aggacgccct tctctttgca ggcttcgtag acgccgaacc gctcggcgaa gatcaggtcc
    77341 gcgcccgcct cgatctgggc gatggcggct tccttggcct tcgggggatc gaaccagctg
    77401 ccgatgtacg agaccttcat cttcacgtcc ggccgcacgg agcgcgcgcc ctcgcggaac
    77461 gcgttgacca ggcggttgac ctcgccgatc ggcagggcgg cgaccgcgcc gatcgtcccg
    77521 gtcttcgtcc gtttcccggc gatgacgccg cagaggtagg cgggctcgtg gatccagttg
    77581 tcgaatacgg acaggttcgg cgcggcgggt ccgaactccg agccgaacgc gaaggcgatc
    77641 tccgggtaat ccttggccac gcgccgcacg gggtcctcgc cggcgaggaa cgcgtcgccc
    77701 acgatcaggt cgaacccgcg gtccgcgtat tcccgcagca ccttctcgta gtcggcggcg
    77761 gcgaccttct ccgtgaactc gtactcgacg ccgagttcct ccttcgcctt caggcaggcc
    77821 tggtggatca cgccgtccca cggctcctcg atggcggtct ggaacaccgc cgccacccgg
    77881 aacgccttcg ccccttcgga ggccggcggc gcgggcggcg agcaggatgc gagcaaggcc
    77941 aggatcagcg cggcggggat catccggagc ggcttcatgg ttcgaatcct ccctttacat
    78001 ggaacgcctt ttccaacacg tcaacccggc cgcgcatcat atcgcatccg gtgcgttacg
    78061 cgccacttat gattgccggg cggccgccgc ggccgtatcc tgatatcccc atgaaccggt
    78121 cttccagcgg ccgtccacgg ctggcggtcg tgatcccgtc cttcaacggc atgccgtacc
    78181 tgccccgagg cggtgcaatc catcctggac caggcctttc ggaaggaaaa tcggaggaaa
    78241 atcggggtca ggccgtaaca atacactttc aggctagagc ctcctccaac ttgggccagt
    78301 cgatcttctt gtcgatgagt cgccggcgaa tcatgtgcac ggcctgcccg ctgatccccc
    78361 cgtagtgcgc accgatctcc cgctgggttc tgccgctcca tcgggaggcc agttccaccg
    78421 ctaccgcctt cgcctccccc gccgagcggc cgtgcctttt cagcgtcccg gcctcgatcc
    78481 cgtacgccct cgctaccacc tcatcgagaa ccgccatctc gacgccgcgc catggccgct
    78541 gaacgtccgc atccctggcc cggcctgtct tcccgcccaa gagctctttt tccaccttct
    78601 cgacgaactc ctcgctaccc caggcatgtg tcgagcgcgc catcagcccc ttcagcggtt
    78661 cgtcatcctc cgccaccatc gcctgcgcat acgcccggta ccgtcgctgc gcctcctccc
    78721 ggctccgccc gtagtgctgc aggaccgcga ggtcgattcg gctcgcggtt cgccggcttc
    78781 ccacgtaatc ggggtagctg ctccacgggt gttcttccag catccggatc cgctcctttg
    78841 ccgtcttctc ctttgccgct cgcgtcttca ccgggttcag gtgcacatac cgcgtcaagg
    78901 ccagcaggta atcgcccgac tccaccagcg gcgctttgta ccgccccccg aacgcatgcc
    78961 cggtcttcgc gtgcttgtac cggtaataga ggctgtaact gctgttcagt cgctgcatga
    79021 accgccccag gttcgcccgc ggcgttcgca ccaccaggtg gtagtggttc gacatcagga
    79081 cccaggcgta caggaccacg ccgtactgtt ccaggttgtc ctgcaactgc aggagaaacc
    79141 gacggcgatc gccgtcggtg tgaaatatga cctgccgggc gttgccccgg ctggtcacgt
    79201 gataaagcgc gttctcgaat tcgttcctcg gtggcctggc cataatgcgt tgctacctag
    79261 ccgaaaccag aggaactgtc aattaaaagt gtattgttac ggcctgaccc cgattccctt
    79321 ctgaccccga ttcgctttgg ctacttggga tctgtccagc gggcgcgttg ttgcaggagg
    79381 aaaagccgca aaccggtctg ctgctccggt gtgaggtaag gcgccatttc gcgcgctttc
    79441 tcgacgacga tccggtggac ctccggctgg agttggctgc gcacgtgttg caggtcctgc
    79501 tggagagcgg cgaggatggg ctcgatcgcg tcttgttggc ggggcgtcag cttcaactcc
    79561 cgtgtcaagt gggcgaggac ggcccggcgc gtggcttccg gattccatgt ggcccattgc
    79621 gcgacacggc gccggacgac gccgcgcgtc accgtgatgc cggagacgaa tccggcggcg
    79681 aagatgagcg cgatgcccgc aaaaatcttc cactttttca tggggtgctc cttcctacag
    79741 aatagaccga agccacaagg cccgggtgag cggcggggcc gcggggtccg tcaggaccat
    79801 ctcgatccgg tccagttcgg atgtccggtt ccaatacaga ttgaggccga gcagcaggac
    79861 cagggccgcg gcgcaggccg ggaccatcca gcgcaacggc gtttcgatcg gggccgtcca
    79921 gagttcctgt ccttccggag gggcgatgcg ccggatctcc cgcatgacgc cgtcctgcca
    79981 gtcgggggcc ggctcgtgcg ccggcggcag gtggcgcgcg gccagtaggg ccgtctcgat
    80041 atgctcgatt ctttgcttct ccttcatggc cattctcctt ttcaacggat ccagggtcga
    80101 atgatctttc gcagcgcgtg gcgggcccgg tgcgcgcggg ccttgacccg ggcggcgctc
    80161 cagttcagca gcctggccgc ttccgagacc gtgcgcccct cggcgtacag catcaccacg
    80221 atggcgcggc tgccggcatc gattcgcgcc agcgcaaggt caagaacctc cgcggcctcc
    80281 cggcgggccg ccgcggcctc gcactcggcc ttcgagccgc cgtccccggc gcgttcgagc
    80341 caggcccggt gctcctccga tagcgcgctc acgggaatct ccgggcaacg cccccggcgc
    80401 cgccagtagt cacaacaggt tcggaccgcg atgcggctga tccaatgctc aaggggatgc
    80461 cgccggctgt acccggccag gctccggaag gcccggataa acgtctcgtg cgccacctcg
    80521 tcggcgtgat ccggcggcac gtggttgagc acgatggaga aaacgcgggc gcgatacttg
    80581 gtcagcaggc gttcgaattc atccacccgc ccctccagga tccggcggat ggtttcgagg
    80641 tcgtcggtct cgatctcggc gccttccatg aacatctccc gctcccccac gatgcctttc
    80701 gtgatcgtcg aatccaagcg cattcgctca cccaacaggc gccgaaactg caggccgcct
    80761 acgatgcgat actaccgcgc cagacgcgat gacatcccgg agcgcgcggt ccgccgtcgc
    80821 gagccggtcc ccgtctccac ccttgaagtc gcgcgcgagc ggcgcgcggt tgcggaaaaa
    80881 aggcaggaag cgatttcctg caaccgaatc agcccgtgcg ccgactatcg atgttgaagg
    80941 gcgggaaacg gcatgaaggt cttctccagg agcggcgagg cggggcaggg ggcgccgggc
    81001 cagatcgtgg acgatgaagc cagcgggcgc cgttacccgg gtccctgttg tcccgatggg
    81061 gggcggtttt gcgcgcggct cgggttgtgg atgattcgga ggggccgcgc gctcggtaaa
    81121 ggagatgcca tgaacacgga cttccgcaag accgttcctg gtggcgggtg ggggggcgtc
    81181 ccggcgttcc tggttgtcgc ttgcggagtg atgatcctcc tggtgcgaac cggttacgcg
    81241 caaagtccgt tcgaaggaga atggcaactt caactgatcc accgcggggg gctctaccca
    81301 aaatggtcca aggcgaacaa cctgattgtc ttcaatgaac tgcgtccggg aaaggctttt
    81361 acggacaggc ttcccgggac ctacgaaatt ttcaccatga aaccggacgg gacgagtgtc
    81421 acgtgtctaa cggacaaggc cccggtcggt ctttcgggct ttcacaagtg ccagccgtat
    81481 tggcatccga gcggcaggtt catcgtgttc accgcgcaga atgagcacac gacgccgacc
    81541 gagcacaaca tggattcctt tcccgggatc ggacataacc acgatgtatg gatcatgacg
    81601 gcggatggcc ggcgttattg gcgtatcacc aggaaccccg ataactgggg cgtgattcgc
    81661 ccaagcttct ctcacgatgg gaagaaggtg tactggaacg aggaatattc catggaggcg
    81721 catccgggca tgggctcgcc gtggaaaaaa gagcgcaatc ccaagggcga agaatggggc
    81781 ttgtggcgga tcaagatggc ggacatagcc ttcagcccaa acggcccccg cctcgcccct
    81841 gtccgaatcg tccctatcaa ccaccttcat cccggctttc gcctgatcga gggatcgggt
    81901 ttcaccccgg atgacgggca cctgatttgg gaggcggctc acctcgggga gacggacggg
    81961 caaatgtggt ggggcgatgt gtatgtgagc gacctgaagg gcggctcctt gcggcgactg
    82021 acacggacgg cgccccttcg ccggggcaat gaaaacatgg agtactcgcc ggacgggcaa
    82081 tccatcgcct ggtcgtatca cgacaacccg gagccgggca agaaagtgga actctggctg
    82141 atgcgcagcg acgggagtga cgccactcca ttgacgcact tcaacacgcc cgggcatcct
    82201 catcgaaaaa ggttccagcc ccttcgtttc accaatgcct gccttgaatt ggattggggg
    82261 ccggacggcc aggcgatcgt cttcagcatg agcaatggcg ccaccctcga atggccctat
    82321 atgaccccga acatatatct gctgaccatg agcggagata ttactaaata gccgacagca
    82381 tccacgcgct tgcgcgcgcg gcggcgtagc cgccccgggc ggggtggccg gcctttcatg
    82441 gccggatgag caacaggtgg aaagaagcgg ggggcgatat ggcgggacac tggagatcgg
    82501 gaaccgcgtc cgggctgatc ggtcgcttcg gccgcatgtt ccccgtgatg gtcagcctgg
    82561 gctgtgcggt ggccggcgcg ggcgccggag aggaccccca tcgtcccgcc ttccatgtcg
    82621 ggtatcagat cctcgatctt ccatgccgca aggaaggagt ggagcatacc cttacggtgg
    82681 ccgtatggta tcccacggcg gcacggccaa ggccgcacca ctatggcggg ctcacccggg
    82741 gccgcgtggc ggtcgatgcg gccccggatg ccgagcgagg cccgtatccg ctgcttgtct
    82801 tttcgcacgg ctacgggggc agcggcctcg gggtggtgtt ccttgccgag gcgctcgcgg
    82861 cacgcggttg gatcgtcgcg gcgccggacc atcatgaccg gcattcggcc gtacgcatac
    82921 gcgaggggcc ggtggaagac tacgaccggc gcggacttct gcggcattcc atgcggatcg
    82981 cggcttccgg ccgccatgac cgggccgact acatgtatcg tttggacgaa atgcagcttg
    83041 ttctcgaccg actgtcggat tcagagccct tcggacgact gacggatccg aaccgtgtgg
    83101 ccgtcggcgg ccattccttc ggcggcttta ccgcgctggg ggtgtgcggg acaatcccgg
    83161 agcgattcga tccccggatc aaggcggtcc tcctgttctc gaccggggcg ggtggatact
    83221 tgttcagcga cgacgagctg gccgcggttc ggattccctc catgctcttt ctcggcgagt
    83281 tggagaagga gtccgcgcgg ggaagcagcg ggacgatggc ggagctttca ggcaaggttt
    83341 acagaaacct gcccgtcccg aaatattttc tcgaagtcaa gggcgcgaac catttgtcct
    83401 tcaacaattc cttcgcgggg cgtacgtcct cgcgtcccgg cgccgacgat tcgcggatct
    83461 tcgatgtcat tcgacgatac tccattgcct ttctggagaa gcacgtggcc gccaggggag
    83521 acgatggccg tgtgctggag cgcggggagg ctatgctcac gcgttatctg aaagagccgt
    83581 agcccggcga acccgccgct tcagtgcctg gccgcggttc cgtggcgcga ttcgcgccgg
    83641 acaagcatca atgaacaagc gggtcgccgt gctggcgttc gccgggcatt ccccgtgcgc
    83701 ggccggcctg aattccggtg gcggccggcc ctcttctgcg ctacgatgcg cgatcatgcc
    83761 gttcggacag gaacagcttt tcgaggcgct tgcgattcgc gtgccgcggc ggcgcgccgc
    83821 gccgtatttc ttcggcgata acgtcgaggg gtacttcgag gggcagacgc accgcaccgc
    83881 ggaaggcgcc ggctacctgc tgcacggccg ggcgttgttc cgcgatttcc tgagctggcg
    83941 cggcgagttg ttcaacgagc gcaccaaggc cgagggcgcc gcgatctacc cgtacggaat
    84001 ccggcacgac cacggcccgt cgtggtggga cgaactgatg ctcctgcgcc ggcagcaggc
    84061 ggtcgccctg cgcgcgttct cccgccaacc gcagacgctg gccctggccc cgctgctgga
    84121 ccttgcgccg cgccacctcg tggtgcaggc caaaccgtgg ggcggcttca tcacggaccg
    84181 gaagaagacg ttctgcgtcg gggtcagttc gagccagccg ttccggcacg acgggaccgt
    84241 gacgcgcggc cgcctggtcg cgcccgtctt tcgcaccctg caggacgaat cggagttcac
    84301 gctgtacctc gcgttcgacc gcgacccgcg ccgcgcccag ggccgggtgc tggccctgcg
    84361 cgaggccgac ggcgtgcgcc ggcacaaggc ggaaatcttc ggcctgctca cgcgcagcca
    84421 cctgtggacc gacgacgagg actacaaccg cgccctgatg tgggccaagc tctcgagcct
    84481 gttcctcgtc gagaacgagt ttggccaggg gatctgggcc ggcctgccgt ggttcaagaa
    84541 caactggggc cgcgacacct tcatcgccct gccgggcacg ctgctcgtca gcgggatgtt
    84601 cgacgaggcg cgcgaggtca tccggaactt cgtccgctgg cagaacacgg acaaggactc
    84661 cccggactac ggccgggtgc ccaaccgggt gaccgggacg cgcgccgcgg acaagatcta
    84721 caacacggcg gatgggacgc cgtggctgat ccgcgagctg tacgaatacc tgtgctacac
    84781 cggcgaccgc gcgttcgggg aggaagtgta cccgttcgtc aggaccgcga tcgcgggcgc
    84841 gctgaagcac cacggcgaca aacagggctt cctgactcat gccgacgccg atacgtggat
    84901 ggatgcccgg attgaaggga aagagccctg gtcggcgcgc ggcagccgag ccgtggaaat
    84961 ccaggcgctg tggttcaacg ccctgctcgc cggcgcgcgg ctcgcggacc tcgtgggcga
    85021 cgcgaagtcc gcccgggagt gggccctcct cgccgcgaag gtgcggaaaa gtttcgcgaa
    85081 gaagttctgg gacgcgcgcg gccggcggct ggccgaccgc ctgcgcccgg acggcacggc
    85141 ggatttcaag gtccggccaa accagttgat ggcgatctcc gtgccgatga tcgagccgct
    85201 cgtggacgac gcaacggccg cgcgcgtgac ggcgaacgca tgcggtgagc tgctctaccc
    85261 gcacggtatc ggttcattga gtcaggacga tccgtacttt cacccgattc accaccgcga
    85321 agagtcctac cacttcgacg cggcgtacca caacggcatc gtctggggct ggaacgccgg
    85381 gcccgccgtc accgcgctgt gccggtgcgg gcagacggag ctcgcgggcc aactggcgcg
    85441 gaacctggcg gcgcaaattc tcgaacaggg ctgccgcggc gcgatgagcg aactggtgga
    85501 cgcctggccg gacgcgaagg gcaagctcgt gttgagcggg tgttgggcgc aggcctggag
    85561 ctccgcggaa ttcgcgcgca acggctacca ggatttcggc ggcttcgagc cgcggctgct
    85621 ggacggccgg atcgtcctgc gcccgcgctt ccccgccgcg tggacgcgct tcgcggcgac
    85681 ctttcccttc ggccgcggcg cctcgctcca gctcacgtac gcgaacgagc atggcaagga
    85741 ggtcttcgtc ctgaccctgg acggcaaaac cgatcccctg accgtttccc tcgatgccgt
    85801 tgccggcggc ttccgcttcg ttgcagaccg ggagatgaag tccgccgaca cgtggatgct
    85861 ggtgatcgaa aaaggccagg ccctctcggg cttgaacggc cggtgggaag gcgcgccggt
    85921 gaagggccgg aagcaggggc gcgcgcggcc gctgggattc gtccggctgg acccgcgcgc
    85981 gaaaccgccc agcctgaaga agaaggacta tctcaaggac atcattctgg ctggccgata
    86041 ccggtaggaa cgcgggccgg ccatctgaac ctggatcccg actcgcacaa cgccctttat
    86101 cttcaattcc gccgggggat tgagcggcgc ttcagcgccg ccacggtggg ggaactgtac
    86161 ggcgattctt cgcgggcgtg ctggagatgc cataccgctc tcgatgtttt ttcgttgacg
    86221 taagcgggcc tgttcaagta ggttgccgcc caaggtagga atggcgaaag ggaggtccgc
    86281 atgaaggctc gttatcagaa gcccgagatt cggaagatca aactggtccc gtcggaagcc
    86341 gttttgcagg cgtgcaagac gcgcggagcc tccggcccgg ccggcggcag ttgcctggcc
    86401 atgaacggga tctgctcggc atacggatcc tgattggatc gctcggagta gcttaggggc
    86461 ctttgtcgac tcgacaaggg ccttttttct ttccgcatga tccggatagg attcatcacg
    86521 cccgcctccg gcccggccga acagaaaagc gtcgaccgtg tctggcgccg gctggaagaa
    86581 gcgctggccg ctgaattcga gctggttcgc tatcccggcg cagcggaggg cgtcgcgtgg
    86641 cgggcgttcc tggaccgcgt ggaggccgtg gtcgttccgc atcccatccg cgcggaccgg
    86701 tccggttcgc ttcgcaagcc attccttcac ctggccctcg gcacgctgcc cctcggcggg
    86761 ctacggttcc tccggatgct ggccgacttg agcaacgcgg attccatcct gatttcctgc
    86821 cgggcggacc aggcgatcgt cgaacgggtt ttcccgcgcc gcgaaatcgg ggcctctttc
    86881 ctcccgtttg gcgtggacct cgacgtgttc cgcccgcttg gcgaggcggc gcgcgaggcg
    86941 gcccgggcgg cgcacgggtt gcaggccgcc gcgccggtcc tcgtctacgc cggccgcgtc
    87001 acccgcccga agaacgtcca tctgctcctc gacatggttg cggcgctgcg gccggaattc
    87061 cccggcatcc gcctggtcgt cgccggcggg ccggagccgc tggattcgga ggctgactcc
    87121 taccgcgagg aactggacgc gcgggcccgg gacctgggcg gcgcggtcgt cttcgcgggg
    87181 ccgctggacg acacgcggct cgcggaactc ctttcggctg ccgacgtgtt cgtcacggcc
    87241 acggtcaacc gcgacgagaa tttcgggttc gcggccgtgg aggcgatggc ctgcggccta
    87301 ccggtggtcg gcacggaatg gggcggtctg aaggacaccg tcgcgccggg cgagaccggg
    87361 gtcctcatgg ccacgcgcgt cgcggatacg gacgtgcggg tggacgcgcc ggcgggcgtc
    87421 gccgcgatcc ggcggctgct ggcggacccg gccttgcgcc ggaacatggc ggaacgcgcc
    87481 ctcgcccgcg cccgggaaca ctacggcctg gactctttcc gccgccacac ggtccggata
    87541 gtccgcgagg ccattgatcg cgcggccggc atcggggagg cgatcccggg cacccggttc
    87601 gagttgcatc ccctggccat ggaatccctg gtctattccc agttgggcgt ccaacgggac
    87661 tggtacggcc gggtccccgg cttcacgccg gaggatgccc ggttgcgccg gtttttcatg
    87721 gagccgtacg cctcggggag cgaaccccgg tgaaaaaggt cctgctcatc gccttcgagt
    87781 tcccgcccgc cgtcgcctcg gggatgtatc acgccttcta cctggccaag tacctccgct
    87841 cgttcgactg ggatcccttc gttttgaccg gcgtcccggg ggccgtgccg gccgcgatgg
    87901 aggatccgct cgatctgccg gccgcgttgg acgagggccg cgtcttgcgg gccggcttgg
    87961 ccgatgaagc ggagctggcg gaattcgcgg gccgtgagtt gccggattcc tggctgcggc
    88021 ggtgtgtcct cggtcaccgc gacgagcacg gcggatcgtt ccgccatgtt ttccccgacc
    88081 cgttggtccg ctggctgccg cgcgcgtggc ggcgggcggt ggagctcctg tcggcgcatc
    88141 gcttcgatgc cgtcgtgacc acgtcctttc ccttcacatc cgtcctgctc gggaggatgg
    88201 cgcgggagcg cttcggcgtg ccgtgggtgg ccgagtttcg cgatctctgg accgaggacc
    88261 cgcgattccc cctgcgatcc gccgccttcg tggccgatag cggacggctg gagcgcgaaa
    88321 cgctcgcatc ggcggacgcc gtggtcgcgg tcagttcgcc gctggccgaa cgcttcgtcc
    88381 gccgcggggg cggggatcgg gttcgttgca tcgagttgag ctacgaggag gcggatttcg
    88441 cgcaggagag ggagccgcca tccgaccggt tccatctcgt ccaggccggg accctttatc
    88501 ccggcatgct gccggccgcg ctgccgaagg ccctggatct tctcgcgcgc ctcggcaagc
    88561 tggacccggc ccggttcggc ttcaccctgg ccggcgcgaa tctttgcggg caagccgggc
    88621 tttccggggc gcgcgtcctc ggccacgtgc cgcacgcgga ggcgttgcgg ttgtaccgcg
    88681 gggcttccgc gctgttgctg accctgcccg cggaacttgg cgagttcatt cccagccggc
    88741 tattcgagta catggcttcc ggcgcgccga tcctggcgct ggtcccgccc gacagcgtcg
    88801 cggcgcggat ggtcctgcgc agccggacgg gaaccgtggt ggacatcgcg gcgggcgtgg
    88861 atgaagtggc cgccgccgtt tatgatctct atgtccgtgg acgagatgga actttgacga
    88921 ccgacccgga ctgggcctac atccgcggct tcagcagccg cgccatggcc ggacgctggg
    88981 cggccctgct aaacggattg cgcgccggcg agcgagtcca gtaggccggc cagttggccg
    89041 gctgtccggc gccggtcgaa gtgttccaga aaggcgcggt cgggctcgac cgcaagattg
    89101 ccgcgcttcc atcgcgcgaa gaaatacgcg atcgcatccg cgatttcgtc ggggcgcgcg
    89161 gggtcggccg cgcggccggt ccgcgatcgc gcgacgtagt ccgccatcag gctcccggcc
    89221 ggggccacgg ccagcacggg ccggccgctg gccatgtact catagagctt cacggaaacg
    89281 gcgtaccggc cgcgccgcgc gtcgctgacg gaaagcagca ggtccgaatc ctgctgccgg
    89341 agcagcgcct cgcggtggga gcccggcgga attttccgga cgagcccgga gagccccatg
    89401 gcctcgtcgt ggcggaaatg ctcgaagtcg cccacgagcc gaaggagcag ttcatcctcg
    89461 cgcgcgagcc gaagggacag catcaggcgc agggccttca ggacgggcag catgtccgcg
    89521 ggattcggca ggtgccccgt gaacgtgatc gtgaaccggt cccgccgcgc cggcgcggac
    89581 ggcagggcgg agaggacggc gccgtcgtac ccgttcggga tcaggtggta cttcgccgcc
    89641 ggcccgccgc ccttttcgac cagcatgtcc acgaccggcg cggtgacgga gacgaccgca
    89701 tcagcctttt cgaggatgtc ccgttcccat cggtccgccc gtgcgcgctg gaagggggag
    89761 cggataaatt ccgggttcac agcccactcg tcccggaaat ccgccagcca cggccgcccg
    89821 gagcgtcggg ccgcctcgcc ggcaaccagg tggttcacga cgggatcgcc ggaggaaaaa
    89881 atcgcggcga cgccttcctc ccgggccagc cggagggctt gcgcgatggc cggccgccgc
    89941 cagcggatgg atacctccgg aaagccccgt cgccggccgc ccggcggcag gggcatccgg
    90001 accaggaccg acgaggcctc gacttccgcg agcagctccg catcgcggct catttccgcg
    90061 ggcgcttccg tggtcagcac gatggggcgc cagccgtgtt ccggcaggta cttgcagaac
    90121 ttcgcgatcc ggatcacgcc gaccccgccg cggggcggga acaggtatga aatcacgagg
    90181 acttttttca tggcggagtc ctccggtcgg cggccgccgg gtcaggtgac caggtccgcg
    90241 atgatccggg agcggccctt caacttcagg atgtgccggt ccagcgcgcg cagttttttc
    90301 atcgcccgcg ccaccaggta agcgcccagg accaccagcc cgaaggacag ggtgaggacc
    90361 agggtcagga agactatgac cttggacacg tcgtacaacc gggacgtggt gatcagtatg
    90421 ctcaacaccg agaggggcag cgcgaatacc gcgatgcccg tgcgcagcac ggacagccag
    90481 gtccgcttct ccgacaggag caactgcacc tcgttcgaca gcaggagcgt ttccgcatcg
    90541 ggttccagtt cgcggggtgg gtgttccgta ttcatcgtgg cggtgagctt gccctgaaag
    90601 cagatgggcg gcaagcccgc aggatgggga gcatttgacg cggcctgagt cggatgctac
    90661 aaacagccgc cccgggcaag gcccgggcga aaaagggggg ggcggtgccc gtcgcgatag
    90721 tcacagaggg gttgaccaag cgcttcccgc agaaagtttc gctgcggcag gcggcgctgc
    90781 atccgttccg gcgcctgtcc ggcgcgccgc tgacagcggt ggaccgcgtc agcctggaaa
    90841 tccggcaggg cgagctgttc ggcctgctcg gcccgaacgg cgccggcaag acgacgctgg
    90901 tcaagatgct ctgcacgctg gtccttcccg attccggccg ggcggaggtc aacgggttcg
    90961 acctgtcgga cgaggcccgc gtccgggctt ccatcggcct ggtcagcggc gaggaccgca
    91021 gcttctactg gcggctgacg ggccggcaga acctggagtt cttcgccgcg ctgcacggcc
    91081 tgtccgcgcg cgcggcggcc gggcggatcg cgcgcgcggc ggagtggttg gaactgggcg
    91141 agttcctgga ccgccgtttc gaccgctatt cctcgggcat gaagcagcgg ctcggcatcg
    91201 cccgcggcct gctgaacgat ccctcgatcc tgttcctcga cgagccgacc cggagcctgg
    91261 acccggtctc cgccgcgcat ctccgggacg tcgtcaagca gttggtccgc gagcgcgggc
    91321 ataccgtcgt gctggtgacg caccagttgc acgaggccga ggacctgtgc gaccggatcg
    91381 cgatcatgca caagggccgg ctgcgcgtcg tggccgaccc cttgaccctg cggcgccagg
    91441 ccggcgtgcg gcactatcgc ctgcgggtgc gcggattgaa cgcggagggc gcggccctcg
    91501 tgaaggggtg gcccggcctg gccatccggg agccggcggg ggacccgggg ctcctcgtcc
    91561 tggaattcca gccggccgag ggcgaagccg gggaagccct ggccaacctg ctcgatcgcg
    91621 tcgtgggcgc cggcgggcgg atcgaagacg tcgagacgcc ggaagccgcc ctggagacgg
    91681 cgttccgcaa gttcacccgt gacgccgcgc cggcggcgga ggcggggtcg tgacgggctg
    91741 gcgaaaaccg ctggcgttcg tgaagcgcga cctgacggtc ctgatcagct acccgatggc
    91801 cttcttcctc cagttcttcg ggatcttcgc gacgatcctg atgttcttct tcgtctcgcg
    91861 cgtgttcggc ggcgccgcga gcccgcacct ggcggagtac ggcggcgact acttctcctt
    91921 cgtcctcgtc ggcctggcgt tctcgggcta catgacggtc gccatgaatt cctttgccgc
    91981 cagcatccgc gagggccaga tgatgggcac gctggaggcc atgctggtct cgcccacgcg
    92041 cctgtccacg atcctgatct cgtcgagcct gtggaactac accttcacct cgctgcgcgt
    92101 ggtcttcttc ttcctcatcg gcgcgctgtg cttcggcgtg gacttgagcc atgccaacct
    92161 ttccgccgcg ctggtggtgc agggcctgac catcctctcc ttcagcgcgc tcgggatcgt
    92221 gtcggccagt ttcatcatgg tcttcaagca gggcaatccg ctcgacgcgc tctcgggcgt
    92281 cgtgttcagc ctgctcgggg gcgtgctcta tcccgtcgcc gtgctgccgg cctggctgca
    92341 gccggtcgcc tggtgcctgc ccctcacgta ctccctgcgc gcgatgcgcc gggccatgct
    92401 gcggggcgcg tcgtggggcg aactgctccc cgacatcctc gcgcttgtgc tgttcgccgc
    92461 ggtgctgctg cccgccagcc tgtatgtctt ccgccgcgcc gtgcgccgcg cgcgccggga
    92521 cgggacgctg acgcagtatt gatgcggttg aaccggagag gcccatggaa cagggagaca
    92581 acggcgtgca cggagggcgg atctctcttc cttcctctgc gccctccgcg tcctctgcgg
    92641 caaaaagacc atgaacgcga cgctgttcca gagaatatcc tggctggccg gccgcgcgct
    92701 gggctcgctg cccgcggccg cgctcctgaa aatcgcgctg ccgctgcggc cggtcgtccg
    92761 cgacgtcgat cccgttcgcg acctggacgc gttggccctc ttcggcgccg cgctgcggcc
    92821 cgcgctgcgg ccccggttgc gggcggcgtg ggagcaggcg ctgcgggacg ctgcgcccga
    92881 tttccgccgc gtgctcgtgg cggccgatgc gcggcggccg gatcggctgg cgggcttcct
    92941 gcatcttcaa aagggccggc cggattggtg gatcgccggc atggaggtcc gcccgctcta
    93001 ccggcgccgc ggcctcgccg agctcatggc ccgggaggcg atccggcgcc tgaaggccga
    93061 gggcgctcgg acgatctgcc tgtccgtgcg ggccggcaac gcgtccgcga tccggctgta
    93121 cgagaagctg gggttccgtc ccgcggaggg gctctccgat cccacgctgc atcccggcga
    93181 cgtggggatg aggctcatcc tgcagcccgg cgccgccact ggctgaccag gaggtccgca
    93241 aagccgcggg cgagttctcc gatccccccg gcgaggtgcc gcccccggcg cgtgaacggg
    93301 ctccgctccg ggtagcgccg cgccaggtac gcttcgtccg gccacaggta gagcagggca
    93361 tccagcaggc gcacgggccg gaacgcgatg tcatgccgga acccggcaag gcggcccgag
    93421 ggtttcgccg cgtcgggcgc gagcgcgcgc aattgccatt cctggagccg gcgggcgagt
    93481 ccgctggccc gtggcgcgcc caggctctcg atcagcccgg ccggcatggg ggcccggagc
    93541 aactgcccgg cgcaacgcag gctggcccgg acggccgccc cgatattcca gcgacgcgcc
    93601 ttgtcggcga cgcgggccca gtccagcgtt tcgcgccccc gctccacggc ctcggccacg
    93661 tcgaggacgt ggatccacca gcccgccgcg aggatccgtt ccgcggcggc tggccggtcg
    93721 agcagcagct cggcgcaggg gccgtgcttg acgaggtgca ggccgagcga cagcagcatg
    93781 tcctccggcg agagcgcgag ggccggcgcg cccgcgattg tgccgggccg cgcgtcgcgg
    93841 aatagctccg cgtagtccac cagccacggc gtgaaccggt ggtccagcgc ccagtggatc
    93901 tccgccatga cgcccgtctt ccggtgcacg tacaggaggt gcaggtgctg ccgctcgtag
    93961 tatccgtcct cgagcgcttt cgcgggcgcg ccgtacccgg cctcgcgcag ctgttccttg
    94021 gcggcgggca ggtcctcctt gagcagcagg agatccagat cgctgtacgg gcgcaacgag
    94081 ccgtcgccgt agaccgcctc gcccacggcc aggccgcgca ggaggatgac gcggaggccc
    94141 cgcgcttgaa agcgctccag caggttgcgc gtttcctgtt cgagccgcgc gccgcgcagc
    94201 atctcctgca ggagcctttg ctggagcgcg gacatggtcc cggggggcaa aaccgcgtcg
    94261 agcccggcgg ccttcaggtt gcggtagagc aggggagcca gccggttctg gtccaccagg
    94321 ggcaggaggc ggaggggatc aagccccccg gccgccgtct cgcgcgccgc cgcgatgtcc
    94381 gcgtcggcgg gccgcgtccg ggcgatctgc gcgaggaacc gggcttcgcg gtgctgatcg
    94441 gctggcggat tcatcttctg ctgggctccc tattagacca gccggcccgc aaacggaagc
    94501 gcaatcatgc ccatttccgg gcttgagcag gggggcgggg gtccggcatg atgccgccgc
    94561 aacagctcaa ccggggaggg gctcatgtcc aaagagaaag ccaggatggc caagacggga
    94621 gacgtggtgc ggcggcagat cgcggacgag atcattcttg tcccggtccg caagtcggcc
    94681 gaggagttgg acagcatcta ttccatcaac ccgctcgccg cccagatctg ggacctgctg
    94741 gacggccaga agacgctcgg cgaaatacgc gacatcctgc tgcagcaata cgacgtgacc
    94801 ccggaggagc tcgaccgcga cctcgccgac ttcgtcgagc agctgaaggc cgaaaagctg
    94861 atcacggaag cctgacatga cggaatcgcg ggttcccgag gtcggctacg agtacttcag
    94921 cgagcgggtc cacaaccagt cccttcgcaa gcgcctcccg ctctccggct cgatcgagct
    94981 caccttcgcc tgcaatttcc ggtgcatcca ctgctactgc gtcgaggagc cgccccgccg
    95041 tgagctctcg ctggaggaga tccgcggcct ggtggaccag gtggcggacg agggatgcct
    95101 ctggatgctg ctgaccggcg gcgagccgct gctgcggcgc gatttcgcgg acatttacaa
    95161 ctactgccgg cggaaggggc tggtcaccac cctcttcacc aacggaagcc tggtgaacga
    95221 gcggatcgcg ggcgtcttga gcgatctcaa gccgttcgtg gtggagatca ccctctacgg
    95281 cttctcgccg gaaacgtacc gcgcggtcac cgggcgggcc gagaacttcg agaaggtcct
    95341 gcgcgggatc gacctgctgc agaaggccgg cgtgccgctg cggctcaaat ccatgatcct
    95401 gcagcagaac aagggcgacc tgccgcgcat ccgggaattc gcccaatccc gcgggctgaa
    95461 ctaccggttt gacccggtgc tcaatcccac gctctcgggc tcccgcaagc cggaggagct
    95521 gatgctcacg cccgaggaaa tcgtggcgct ggatgtgggc gacccggagc gcctgaagag
    95581 ctgggacgag ttctgcgaga agttcacgtt cgcctccgac gacgggcggc tgtacaagtg
    95641 cggggcgggg cagtggagct tccacatcga ccccgagggc aagctctccc tctgcatgtt
    95701 cgcccggcag aaccaatacg acctgcgaag cgggaatttc cacgaggcct tctacacctt
    95761 cctgccgacg gtgctcgggc agaaggaaag ccgcgtaacg aaatgcggcg cctgtcccat
    95821 ccggaacatg tgcgggcaat gcgccggctg ggcccacctg tacagcgggg acgaggaaga
    95881 gccggtcgag tacctctgcc ggatcgccca tgcccgggcc aacgctttcg ggctgaacaa
    95941 gataggatct tcagcgcccc cggcgtccgg aaacgcttga tattgtgttg acgctcggcg
    96001 cgctctgtgc gaatatccat tcccgcttgg atcagggagt ccaacaagga gagcgagcat
    96061 gaagaagacc tacaagaaac ccgagattcg caaggtgaag ttggtcccgt cggaagccgt
    96121 gttgcagtcc tgcaagacga caggagccgc cggcccggga cagcagtcgg gctgcttcat
    96181 cacactgatt tgctctatca tcggttcctg actcggatcg ggatcgcctg aattcttttg
    96241 aggcctccgc ttcggcaggg gccttttttc ttgaccggta cagggcgctt ctctagcgtg
    96301 cgatcaccga gggaggagca tgatgaagaa ggcctacagg aaacccgaga ttcgcaaggt
    96361 gaaactggtc ccgtcggaag cggtgttggc ggcgtgcaaa acgcagggcg tcagcggtcc
    96421 cgatgtctcg ggctgtcagc cggtttcgca tatctgtgct tctatcggct cctgaagccg
    96481 cggcgaggat ggtaaaaaac aacgaggcct ttgcggaagc aagggcctcg ttttgctgtc
    96541 gacgggaggt gggcatggtt cagaaggctg tctttgatat agccggcatt acctatgtga
    96601 tagaaaaggc cccgggcggc gcggaattgg atgtcgggcc ctcgcaccag cacttcctct
    96661 cggacaagga gccggccctg cgcttccggg ccgtggacgg gccctggccg tccctggagg
    96721 ggttgaagaa aacgttcgac tccacgtcct ggtggtccat gtccgaggcg ccgggcgccc
    96781 gcgtggtctc ttttttcaac gccacgccgc cctacgagga aaaccgccgg gccgtgctgg
    96841 ccccggacta tcgctccggc gccgtctatt gccggaatgc cctggatcag tcctttcccc
    96901 tgaactaccc gctggaagag ctgatctaca tcaacgtgct gccctccttc gacggcgtga
    96961 tcatccactc ctgcggcgcg ggggacggga atggcggcta cctctttgtc ggcgtctccg
    97021 gcgccgggaa gagcaccatc tccaaggtgc tcatgaaagg caccgactgg aaggtattga
    97081 gcgacgaccg gatcatcctg cggaagatgg agggcggcat ccggatgttc ggcacgcctt
    97141 ggcacgggga agtggatgtc tgcgagaaca tggggatccc cctccgccgc atctacttcc
    97201 tgaagaaggc gccccgcaac gaagccgtcc gctgggcgcc cgcgctggcc gcgacgcacc
    97261 tgatcgcccg ctcgttcccg ccgttctggg atgccgacgg cctggaccgg accacgcgcc
    97321 tgtgcattga tatcgccacg caagttccct gctacgagct gggattcgtg cccaacgcgt
    97381 cggtgctgga ctgcctggcc gggctgaagg acggttgacc ggatggagcc ccggcgccct
    97441 cgccgggcct cccggaggat ggttgtggcg gattcccgcg atattcagcg cgtgctgctt
    97501 gccgagggca acgaggtcat gctgcggacc gccggcttga gcatgggcca cgcgatccgc
    97561 ggcggcgagg ccgtcgtcat ccgggccgtg cccgagcggg acatccgctt cggcgacatc
    97621 ctcgtcttcg aacgcggcga ccagctggtc tgccaccggg ttgtgggcac ccgggcgcgc
    97681 ggcgcgggcc gggagttcgt gaccaagggc gatcccgtgc tcggcctcga tgaaccgatc
    97741 ccgtatgccg cgattctcgg gcgagtggtc cgcgtgaaat ggccgggcgg cgacttccgg
    97801 ctggacaccc ggcgcggccg gtggagcaac cggttcctgg ccgtcgtatc gctgggcgtc
    97861 gccgggttgt accgcgtgtt tccctggaag ggtgcgctga agaaaccggg gagcggcgtc
    97921 cacgcggtgt ggcgtcttct cgcgttcccg tcccgcgtgg tccggtggat cagcgagcgg
    97981 tgccgctgac ggttactccc gcgccgccgg cagcagcgcg tggcagagca cggcagtcag
    98041 tccgcccgtg accgcgccgg aagcgaagac gtgccgcgcc cattccggca gggccttgag
    98101 cgcgtccggc gcgagcacca cgcccatgcc cgtgcccagc gacgccgcga ggatgagcag
    98161 ggcccgccgg tcgagcggct ggctcgcgat gatccggatg ccggccgcgg ccacggtgcc
    98221 gaacatcagc agcgtcccgc cgcccaacac cggctcgggc atcagggcga acacgcccga
    98281 gaccagcggc gagagaccca gcagcaccag caggccggcg atccagtggc ccaccgtccg
    98341 gctcgcgatg cccgtgagct ggatcacgcc gttgttctgg ctgaacgtgg tgttcgggaa
    98401 cgtgttgaag accgacgcga gggcggagtt gaatccgtcg cccagcatcc cgcccgcgag
    98461 gcgcttcagg tacagcgggc ccgcgatcgg ctggcgggaa acgatggagg tcgcggtgat
    98521 gtcgcccacg gtttcgatgg acgtgacgac atagagcagg gccacgggga tgaaggcgga
    98581 ccagcggaac gccaggccgt actggaaggg gagcggcaga accacgcggt cgagttccgc
    98641 gatcggccgg aaatccaccc gacccagcag cgccgcgacc gcgtagccgg cgagcagccc
    98701 cgcgacgatg gagcccatgc gcacgagggc gttccggctg cggttcaaga ccaggatcac
    98761 ggcgagcacc agccccgcca gcgcaaagtg ccggaggtgg ccgaacgagc cgtccgccat
    98821 ggccgcgccg ccgcccgcgc agttgaaggc gccggccccg accaggctca acccgatcag
    98881 cgtcaccacg atcccgctga ccagcggcgg gatcaaccgg cgcacgagcg ggatgaaccg
    98941 gctgatgatc atctccacgg ccgagcccgc aaggcacacg ccgaagatca gcggcaaccc
    99001 gccggtctgc ccgatcgcga tcagcgcgcc gacgaaggtg aagctggtgc cctggatgct
    99061 caacaggcct gacccgagcg ggccgacctt ccggacctgg atccaggtcg agacccccga
    99121 aatgaacaag gacaggccga cgatggttgt cgtggtgggt acatcgcatt tcagcgcgcc
    99181 gcagatcagc agcggcggcg tgatgatcgg cacgaagacc gccagcaggt gctgaagagc
    99241 cgcgaacagc gcgatccaga acggcggccg gtcgtccagc ccgaacagca gctcgcgatc
    99301 cccgcggtgg ggttcatcgg acggcatggc gttcaggccc ccgtcccctg ccgggcgctg
    99361 gcggaccgca gcatggcccg cagcagcgtt tccgcgccgg gggccgtttc ccggatcacc
    99421 tcgatgccca gcgcggggtc ccacgaggac tggtcgtcgg tcagcaggat cagcccgctc
    99481 cgggggcgca gcttgcgaat ggcccgcagc agggccgggg cctcctcgcc cagcagcttc
    99541 cgctccagca ccagcgcggc caggtcgccg ggcgcctcga cggcctggag cgtggcgacg
    99601 atgtcgtcgg ccgtgcggac ttccatgttc aggtcgcgga acagccgcgc ccaatcgtcc
    99661 cgcgcggtcg tgcgcgcgcc ggccaccagg attttccaca tggagacgag cagggcctcc
    99721 tccgccgtga gcgcgcccag ggccatgctc ttttcggcca ccggcaggct gggcaggcac
    99781 agccggtacc cgtgccggcc ctcctgtgcg aggacatcca gccgcccccc ggcttccccg
    99841 aggatcgagc gcacgacgga caccaccacg ccggcctcca gcgggatggc cttgaccggc
    99901 gccgacgagg cgtcctcggc ttccgacgcc gcgatccaca cggcggcggc gcaccgggtg
    99961 ccgacgtcga acaggggggc ggcggcgtca ggcggccgcg cgcggatgtg gagatgcccg
   100021 ggatgcccga gttcgtcggc ggccagcagg gccaggctca agatgatctg ctcaatctgg
   100081 tttccggtca gcagggtcgg accgagtcct ccctggatgt ccaccgccac gcgccaccgg
   100141 ctggaaaggc ccaccgtgag cagttgcgtt gcgcgctgga gctggtcggc caggcgcgtg
   100201 cagggctggc cgcgtgcctc ggcctggctc aagcgcagca cctgcgcggc gagggacgcg
   100261 ccgcgccggg actcgtgcgc gatggcctgc agactggccg cctggtccgg gcccgaggcg
   100321 ccccgcccgg cgagttcggc gtgccccgag atggcgcaga ggatgttgtt gaaatcgtgg
   100381 gcgacgccgc gcgcgatgcg cccgatgagc tgcaaggtgg ccatctgcct cgaccggatt
   100441 tcctccgcct tccgctgggt gacatcgctg accaggatca gctggacgtc ctcggacggc
   100501 tgggagcgga aacgcagcgc gcgcaggccc tcggcctcgc gcacgaggca ttcgacttcc
   100561 tggggttcgc ggccgcccag cagcagggac tcctgttcgg ccgacagcgc ggtgaacagg
   100621 tcccgcagcc ggctcttgga gtcgggctcg gcgcccgcaa aacggcgggc cgccgggttg
   100681 gacccggtga tccggccccg ccggtccacc gccagcaggc cgtcgctcaa gtactccatc
   100741 gcgtccacga accgcccgac gcggcgacgg ctttccacct gcgccgtgga ccggatcgcc
   100801 cagacgccca tcgcgccggc caggaacagg aagcccatcg tcagcgccag gatcgtcagc
   100861 gtgatgatgc ccaggtagct gcgcagggct tcaggcgtgg cggaccgggc atcggtcagc
   100921 aggaacttct cgatcgagga cagggtggac atggccgcga ccgcggccag caccagcagc
   100981 agcagaatca tcaccgcgta ccgccatgtc ccccgcggaa accaggccat ggaattcctt
   101041 tccgattccc gaacctcgat acgcctgatt gtagccggga ctttcccccg gcgcaagaag
   101101 gccgaggcgc tggacaggcc gcgtgcggcg tgtatagtgc ggctggatcg acacgggcgg
   101161 tgggaatcat gcgcatacct ttctggaaaa tgcacggggc ggcgaacgat ttcatcctgg
   101221 cggatgaccg ggccgggacc tttcccgcgg gcgatcgcgg ctggctggcc cggatcatgg
   101281 cccgccggac cggggtgggg gcggagggcg tgctgctgat ccagccgtcc gcccgggccg
   101341 atttccggat gcggttcttc aatcccgacg gcggcgaggt ggacatgtgc ggcaacggcg
   101401 cgcgctgcat cgcccggctg gcgcacgagc tcggtgccgc gccgcgcgag atgcgtatcg
   101461 agacccgcgc cggcctcgtc cgcgccgccg tggacggcga cagcatcctg ctgcacctga
   101521 ccgaaccccg cgactggcag ttgaaccggc agttggaggt cgccgggcag atcctcgacg
   101581 cgcactccgt caacagcggc gtgccgcacg tcatggtcga agtcccggac ctcgacgccg
   101641 tcaacgtgca ggaactcggc cgggcattgc ggtaccatcc ggcctttgcg ccgggcggga
   101701 ccaacgcgaa ctttatccag gtcaccggcc cccactcgtt gcgcattcga acctacgagc
   101761 gcggcgtcga ggccgagacc ctggcctgcg gcaccggcat cgtcgcgtgc ggcctgctgg
   101821 ccgggcgcct gggccgcgtg cggcctcccg tctccatcct taccgccggc ggcgacacgc
   101881 tggaagtcaa cttcgcgccg gcggccgacg gcgcgaccgg ggtcacgctg cgcggcccgg
   101941 ccgtgcatgt tttccaggga acgctggagt atccttgaag gcctcgccgg tccaatcccg
   102001 taccgtcctg gtcaccggtt gttccaccgg catcggcctt gcgaccgccc gcctgttgcg
   102061 cgaacgcggc tgggccgcct ggcccaccgc ccggaaggcg gaggacctgg cccggttgcg
   102121 cgcggaggga ttcaatccca tcgaactgga cctgtacgat ccggcctccg tctgccgcgc
   102181 cgcggaggag gcgttgaagc ggggtggcgg ccgcctcggc gcgctggtca acaacgcggg
   102241 ctatggccaa ccgggcgcgg tggaggacct gacccgcgaa gccctgcgcc ggcagttcga
   102301 ggtaaacgtg ttgggcgtgc aggacctgac caaccttgtt ctccccgtca tgcgcgggca
   102361 gggctacggc cgggtcgtca atgtcagctc cgtgctcggc cgcctgacgc tcccgttcct
   102421 cggcgcctat tgcgcgagca agcatgccct cgaatcgctg tccgacgcgc ttcgcgtgga
   102481 actgcgcggg accggcatcg cggtgtccat catcgagccc ggcccgatcg agacggcgtt
   102541 ccggaagaac tccttcgagc aggggcagga gcgcctggat atctcgaagt cgcggttcgc
   102601 cgagcggtat cggagcgaag gctcgcgccg ggccgcgaag aatttccgca gctcggatcc
   102661 tttcacggcg ccgcccgaag ccgtcgcacg gcggattgtc cacgcattgg aatccggccg
   102721 gccgtggcgg cgctatcccg tgacgctgcc ggcgtgcgcc ggtagtttgc tggccagatt
   102781 ccttcctcac gccataatcg actggctgct gtcccgccgc atgggcaagt cgtaacaatc
   102841 tccctcggaa agaaatactg cttcggaatc cggaagcgga aatccggact gaaaagccta
   102901 tacaggcatt gacgaaacga gtattaagta aagcgtttta cttggggtgg aaatgaaaaa
   102961 gcatctagcg attatcgtgg cgctcgtcgg actgattgcg ccttcgttgg ctgcaaccga
   103021 gaccatttcc gtgtccatgg acatgcccgc tgtttccatt ggaaacgaaa caggtgggtt
   103081 cgtcgatgtc ctggccgagg ggcggaattc cgatggtcga ccggcccgcc cgatgctccc
   103141 cgggaagtct gtcttctacg ctctcccgcc cgacgtggac gccgcctccg tctctattgt
   103201 ggtcacgaag acggagtacc gcgac
//
LOCUS       DAOOTK010000002        92821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_200_length_92821_cov_6.584363, whole genome shotgun sequence.
ACCESSION   DAOOTK010000002 DAOOTK010000000
VERSION     DAOOTK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 92821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 92821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92821
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_92821_cov_6.584363"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PK634_00430"
     CDS             <1..753
                     /locus_tag="PK634_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOW96230.1"
                     /translation="RTLDLGGDKFASTLKMPTELNPFMGWRAIRFCLAQPDIFKTQLR
                     AILRASVHENVKIMYPMVSNVGEVMRANAFLQEAKNELRLRDVPFNDAVEVGVMIEVP
                     SAAITAHLIAPHVAFFSLGTNDLVQYTLAVDRVNERVAYLYEPTHPAIIELIRNTINV
                     AHQHGIWVGICGEMAGNPVMTPLLLGLGVDELSMSPSAIPLVKEVIRSLRFAEAEGLA
                     RKVLPSVLATEVLEACRDLTRRVAPDVLELVG"
     gene            799..1977
                     /gene="metK"
                     /locus_tag="PK634_00435"
     CDS             799..1977
                     /gene="metK"
                     /locus_tag="PK634_00435"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOW96231.1"
                     /translation="MSHSSYLFTSESVTEGHPDKLCDGISDSVLDACLAQDPRSRVAC
                     ESLAKTGMVVVAGEITTQAVVNFSDVVRQKVLKTGYTSSDIGFDGHTCAVIVALDRQS
                     PDIKQGVDAKKAEGKSTAEQGAGDQGMMFGYACDETKELMPMPIMLAHKLTRGLARVR
                     RSGALPFVRPDGKSQVTVAYEDNRPVRVDTVVISTQHSPDVSHKKLSEAIIDELIRKE
                     IPAKMMDRKTRFLVNPTGRFVVGGPQGDCGVTGRKIIVDTYGGMGRHGGGAFSGKDPS
                     KVDRSAAYMARYVAKNIVAAKLARRCEVQLAYAIGYPEPVSVLVDTFGTGIAPNAKIE
                     KAVRRVFGLKPAEIVRGLNLLRPIYEATAAYGHFGRTTDLDNFTWEKTDKVDALLSAL
                     "
     gene            2105..2455
                     /locus_tag="PK634_00440"
     CDS             2105..2455
                     /locus_tag="PK634_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96232.1"
                     /translation="MKGQLAWWVCLGLAVILAGCASPGKNVGKLRLGDTPDVVLDKMG
                     KPNTVRAAKVYEDESSLEVWEYLPGVFTLYPKAYWLFVEDGRLVQWGEPGDFAGKAGG
                     PIPVGEYSDKKRLD"
     gene            2538..3890
                     /locus_tag="PK634_00445"
     CDS             2538..3890
                     /locus_tag="PK634_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96233.1"
                     /translation="MTHRIQQSGPPGAGRIGRLWRTALLGGAVCGLAGPASAQFLRLG
                     IFDVTAETGIDLIYTSNVEGQRPSEAEYDMTDYYMVPRLQLLGEAPFGRGTQLTLDTN
                     VGFEKHLIRTDLDNENAPFGKLRLDTRTELARYQVDLYAGIGREYKDAEDIYVPGDRK
                     TRDVTDTHEYGGEVLWTWRALTLGGRYEFTGERHLDPAFEDGEQDETIVGWNAACQLR
                     EDLALNYDYQRTRTVLINDPEDPTWEIEESITVDWQLPLWERPTITYSFGFEREGVVE
                     DPQDRSGDWDIIHTLTADGVWDLSPTLMFSLHASYEYESDPEENDVSLTYGLELAHEL
                     SGSARQTLTAEREPVSTFGSTAETDKTTIEYIFTKSDLFIYDLDLDTGVGWELNVPLE
                     EGEDVEEIWDYHVGLTHSRTLSRRLVRDVSYMYTCEKSSLEDELLQEHRVTLTFTYTF
                     "
     gene            3918..5105
                     /locus_tag="PK634_00450"
     CDS             3918..5105
                     /locus_tag="PK634_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOW96234.1"
                     /translation="MANHRIRNGMRRALCVAAMLGLAAGAFAQEPKPSLKGETVRRIM
                     AGDRLRVSVLEDPSLNQIYTVAGDGTVDLGFVGRLTLEDLAIDEAADMIERRLEETYF
                     KQATVTVEVSEFVEGAILVVGAVVNPGATPFRGDQMMTLMEVITMSGGLTREAAGNEV
                     RILRWKPGGGMARQTLTVDVQTMLETLDFSRDQFLRPRDIVFVPSLGASENRKEFLAL
                     GAVGAPGFHPYSEGLDVIRAVMRVGGIDREGVWSAARILRPDQSGTYQVIPVDLGRLF
                     GAAETSLNAPLLPGDIFFVPFAEQASRGQVYLLGEVAKPGIASLGMGQDTTLARLILT
                     SGGLGKFANDSKVRILRSAPDGTRQTLYVDVGRIIKTGNFEEDVPLQNGDVVIVPEKL
                     LSF"
     gene            5117..7405
                     /locus_tag="PK634_00455"
     CDS             5117..7405
                     /locus_tag="PK634_00455"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOW96235.1"
                     /translation="MIQPPSSAGAAGAPPASRPETPAPLPSRSVDIFDIKQYFHVILK
                     RIWLVALCFFVSVGVTMVFALQEEPVYRARATLLLSQGLPLPAILRQEEMQLRGEYLA
                     TQQLILQSGMLKERAKERLARAGEKQGVYDELRAFQVSETSFIAIEAEGPDPLYASAF
                     VNALAEEYLDFKAEERMDTSQATVISLTQQANRLLEELKRAEARMVAFEKDNSVVALQ
                     DRGNVAASLMAELSRRASTYRMERMLLESQRPLLTGASDDIILNTLAWPSAAALRPAS
                     APPAGAGEDKAAVAEGAEPAAERPERVLEWGVVERPGWSDLKRERARLEAQLAQYREK
                     FKDEHPAIRQTLDDLAKNQQALDVEVQFAMSQFNAHLESLTLQEQATRRVEKDWEADA
                     IDSTRKAQEYRALQRNAERLQGLYDLVFNRLKEIDVSIGIEPETERIMERAKPPTSPI
                     TTRKVQSIVIAALVGLGIGLALVFGLETIDDSLKYPEEVTKWLNLPFLGVVPTAKWDP
                     QDLRTHVLANIDQKSVLAESYRNLRSAFLFSGASDRVRTLLMTSAVPQEGKTTTCLNL
                     AVSLAQVGQRVLIVDGDLRRGELHKFFGLEGGRGLSDVLVGQAKPDAVIQRTGLPNLD
                     FVATGPLPPNAAEIVLRPEMNSFLDYARRTYDRVVFDCPPVMAVSEAVILASLVDAVI
                     FVVWAGQTSRKLSGLAIQLLRERGSHLLGCVLNNLEFGRVGYYYYSTYYGYYDYESGY
                     SGSGTRPGAAAAAANGGYRKSR"
     gene            7436..8899
                     /locus_tag="PK634_00460"
     CDS             7436..8899
                     /locus_tag="PK634_00460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOW96236.1"
                     /translation="MLGKSHIRISLRRIVLLALDFLCVAGSIVLAAFLRLGPGDGWDY
                     VLHHRLTLGAACAMFLLVFYSGGMYEREPLLKRKRELAQLPLLTTAIALILIVVLFYA
                     RAGASIGRGILLLAGGLIFLGSWGLRTLYRIGMGYGLLSRNALIIGEGNEAREVLRLL
                     AGAPDAGYRVFGIIFGSRTKHEDLVEGIPVLGHISRLNEFADAYAVDTIIVATSLARE
                     HALLRLLRPLRYAGVAIVDYVGLHEELSQEIPLDHIDDEWLMYAAMNNSVIHIRKIKR
                     GMDIVVALIGLALSLPISLLAALAVKLTSRGPMLYRQRRAGQGGQPYTLMKFRTMRQD
                     AEALTGPVWADKFDQRVTPVGRFLRKSRIDEIPQLVNVLRGEMSLVGPRPERPEFVDT
                     LAAAIPFYKERLLVPPGVTGWAQVKYPYAASIEASRRKLQYDLYYIKHMSFVLDILIL
                     LRTFKTILAGLRHSEDLEAAATPAAPADPTEGQAKWA"
     gene            8920..9453
                     /locus_tag="PK634_00465"
     CDS             8920..9453
                     /locus_tag="PK634_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOW96237.1"
                     /translation="MNSTRQPPPPEIGEPGPGEAWVVLHTRPRCEKKLEAFCRRAGMP
                     AYLPLHRKVHRYGARERSFWSPLFPGYVFCAATAAGRSTLRQNQHVANLLEVLDQARL
                     VGQLEQIRQALAVDDVIEVMPYLAAGRRVQVTAGPFKGLEGVVQRVKGRTKVVLNVDM
                     IEQSVCVEVDSTWLASA"
     gene            9450..10289
                     /locus_tag="PK634_00470"
     CDS             9450..10289
                     /locus_tag="PK634_00470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOW96238.1"
                     /translation="MKEATKWQRDADFPNPEPTPMLSFLKQLVRRYAPAGLKHRLRKW
                     NSWRLVRHFDEGRWPPAAMARRLVRPGDRVVDAGANIGYVTALFSKWVGPAGRVCSFE
                     PEPGTFDVLASNVRRLNLANVSLFPCAVSSREGEGVLAIPEYAAGGENLYEARVVDAP
                     ARARGIRTVAIRLGALDKLLEGLDSLALIKLDVEGYELEAVRGGAGLIGRFHPALLIE
                     VSGDPWTPGTKAAALFELLKGWGYSPYRSEAGVWRKLQAGDAVLDVVFLAGPPPGESP
                     VHH"
     gene            10437..10895
                     /locus_tag="PK634_00475"
     CDS             10437..10895
                     /locus_tag="PK634_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOW96239.1"
                     /translation="MIEAGGRTAQSFGLGRLFGQIFMLLYLSPEPASLDELASQLGVS
                     KASISIACRQLETWGAVHRVWVKGDRKDYYVAETAFDRILRSGLLLSLNKKLDTAKVH
                     IEQSLALLEKSGAKDNGAAFLRTRLRQAEQYRERINRLLGSRLVRAILRI"
     gene            11035..12345
                     /locus_tag="PK634_00480"
     CDS             11035..12345
                     /locus_tag="PK634_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOW96240.1"
                     /translation="MSLWSFRFLLWYICILIVQPQHRFSFLMPFRIANLCMVIATGLH
                     VASALIEGRPILRFGPATKLALALLGFGLLSQYAGAMQTYTGWNSFIDMLVKNAYMLI
                     LIEAMAVTVERVWAVPTTMALASLWWIKGGLRLSQAGATWGMGDRIMGPASGLVQDPN
                     FFAYMMGVFMPLYLYYSRSGDRKLVRWAGLAVAIASFFISLRTGSRAGLLQLFALGAL
                     LLPRYWFHYRRALLIAVAAFFFLIPLSGRQNIARFKTLPASIAAFFKGKAEKGAGPDA
                     YSAMERRTKIADTWALIQEYPVFGVGMNPDESLFRDKYWGATAQVHCEILMAGRQMGF
                     IGMAMHVGFLLIPFLIGRSIQKRWAGAWPALADLGYTVKVMALMFAIGGSFLPLPWHP
                     ILLALNGTASALYLNLKALEEAERAGVAPAGRVPDTAGPLPTTA"
     gene            12359..13234
                     /locus_tag="PK634_00485"
     CDS             12359..13234
                     /locus_tag="PK634_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96241.1"
                     /translation="MSAPLLTVYESSNTDRLSISAWREMLAEFWSYRELTWRLMSRNF
                     AAQFRQSFLGYLWVILPPVATTIVFALLRQAQIVNVPMAEGAMPYVLFALMGTTVWGF
                     FTQVTITTTHSVAGAGNLVSRIYFPREVLAVSSALNAVINLCIRLVVLALTFALARYV
                     PHWQVVFAPLLLVPLMALAMGLGLLFAPVNTMMDDMGRMLDFAFQFGLFLAPTVYPTP
                     RLADITGKWELALFWIHSLNPVSHFLHAMQSLVETGTFDVTRGLVVSTILSFLALAAG
                     WRFFHICEPLLAERL"
     gene            13268..14635
                     /locus_tag="PK634_00490"
     CDS             13268..14635
                     /locus_tag="PK634_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96242.1"
                     /translation="MSSEPAIRVERLSKKFARSLKKAMAYGLVDIARVAFLPRRARSP
                     QFAARVADAASAPAPAADVAEGLRPSEFWAVRDVSFEVRSGECLGLIGANGAGKSTIF
                     KIMSGIYGPTSGRVEIRGRLSALIEVGAGFHPMLSGRENIYISGAVLGMTTAQIEKKY
                     EQIVDFSGVRDFIDMPVKFYSSGMHVRLGFAVLAYLEPEVLLIDEILAVGDMEFQKKC
                     IDRINEMRQSRMAIALVSHSLYRIESLCHRAVWLDHGKVVLSGPARDVVRAYRDRQIL
                     LSADEARAKQGGGAAAVAESPFFTVHRADPLHEDGRADARFAFGETLAIRLAFTAKQR
                     IERPLFNLAFVAAGVRVLETSMLVDGQAPECVEGPGELVVRLPAPRLLPNAYTVELFI
                     RNREGMVDLIDTQTVTHFTITSDGLEAIPQAGPYALGHLMHGNIVHQPYEWDLSRIRQ
                     PEEAR"
     gene            14632..15852
                     /locus_tag="PK634_00495"
     CDS             14632..15852
                     /locus_tag="PK634_00495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOW96243.1"
                     /translation="MNGILRGWRGVAAAALALALLPGCASGPSTTGLKVLARKGRPFV
                     IVQTDRAKGWGVANHPILCRVSDRRLALSYWIAGDAVTEGTAPVGWPAYSDDDGRTWQ
                     FGDPYAWLGEPPPPEARVIEAGSRFAHYDFGYFMAAAVLPSGERVHHDRTAVLAPTGS
                     LGSIRSADGVHWTSPERVPLDVPLEGGAAPANLYFEQQAVVSTGGVLYVAGYGQVPSE
                     ECYATFLFESRDGGRSYRYVSKVASMAQAPWGIEGADEPALVLLPDGELLCVMRTGAP
                     WSGGGISVSRDLLSARSRDGGRSWEYRRLPIAGVMPKLRLLTNGILALATGRPGNSLF
                     FSADGGRTWGREVVLSRADQKTSGYCDLVEVSPGRLLAIYDSMDTDLSGIWLWEPKEA
                     NGIFGVYIHVTRLF"
     gene            15880..16929
                     /gene="pseB"
                     /locus_tag="PK634_00500"
     CDS             15880..16929
                     /gene="pseB"
                     /locus_tag="PK634_00500"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOW96244.1"
                     /translation="MITMRPLDFLEKNRVLITGGTGSLGKKLTRIILDHTATTVVVIY
                     SRDEYKQFEMELSFTPAQRQRLRFFLGDVRDGDRLRRALDGVRFIIHASALKQVPAAE
                     YNPFEFVKTNILGAQNVIDAAIDSDVTHVVALSTDKAANPINLYGATKLCSDKLFVSG
                     NSYSGRRPTRFSVVRYGNVVGSRGSVIPFFLARRREGVLPITDPRMTRFWITLEQGAA
                     LVLNALQDMKGGELWVPKIPSMRITDLARVIGPECQTPVVGIRPGEKLHEVMIPVDDG
                     RQTIEFNTHFIIRPAFPWWGENWHMEKGGRPCPDGFYYGSDNNTDWLDDARLARMIAD
                     LDLPEAQAWAKERGI"
     gene            16999..18141
                     /gene="pseC"
                     /locus_tag="PK634_00505"
     CDS             16999..18141
                     /gene="pseC"
                     /locus_tag="PK634_00505"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOW96245.1"
                     /translation="MIPYGKQFLDAEDIQAVVDVLKSDWLTTGPKVAEFEKALAAFTG
                     TKEAVAVSNGTAALHAAVYAAGIGPGDEVIVTPMSFAASANCAVFQGATPVFADVREE
                     DLLIDPGKVERAITGKTKAIIAVDYAGLPCDYDSLTALARARGLVLIADACHAVGGAY
                     KGRPVGSLADLSTFSFHPVKNMTTGEGGAITTDNEEYARRMRMFRSHGVTVDFRQRME
                     SHSWFYEMVDLGWNYRITDFQCALGITQLKKLPGWITRRNEIAARYDAAFLPLAPVVR
                     PVHPSSLILHPASVHGRHLYVIRVPAARRAEIFGALRKQGIGVNVHYIPIHLHPFYRR
                     RFGTGPGLCPAAERAYEELISLPMYPAMTDGEVAAVIGGVRHALGG"
     gene            18144..18791
                     /locus_tag="PK634_00510"
     CDS             18144..18791
                     /locus_tag="PK634_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid biosynthesis-associated
                     methylase"
                     /protein_id="HOW96246.1"
                     /translation="MSQKKKKTRRARTEQEAFWAGTFGDDYIGRNRSDQLLAGNLALF
                     SRILARARDVGSVIELGANIGMNLKALRALRPGAACTGVEINAKAAREMAAIPGVKAV
                     RASLFDYEPKPHDLAFTKGVLIHLNPEMLPLAYDRLARAGRRYVAIIEYYNPAPVEVP
                     YRGHSGRLFKRDFAGEFMERHPAFRLVDYGFVYRRDPVFPQDDLTWFLMERRGSR"
     gene            18788..19486
                     /gene="pseF"
                     /locus_tag="PK634_00515"
     CDS             18788..19486
                     /gene="pseF"
                     /locus_tag="PK634_00515"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HOW96247.1"
                     /translation="MKAVALIPARGGSKRIPGKNIKPFCGRPMIAYSIAAARDSRLFE
                     RILVSTDDEAIERVARDEGAEVPFRRPAELANDHAGTDDVLVHALDWLAGQGGAPRYV
                     CCLYATAPFVTGADLQRGLDALQKAGAITAVSVTTFAYPILRALKVSATGRLEMFWPE
                     NRTRRSQDLPEAWHDAGQFYWLDVPRYRAEPRLFSSDSVPVPLPRHRVQDIDTPEDWA
                     RAEAMFQALLPSPS"
     gene            19630..20016
                     /locus_tag="PK634_00520"
     CDS             19630..20016
                     /locus_tag="PK634_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOW96248.1"
                     /translation="MEYEDLTEKIIGCAYKVYNRMGFGFLESVYEKCILIELRKTGLT
                     AESQVPIRVRYEGEVVGDFIADILVQQTVILELKSVRQLATAHEVQLVNYLVATGKPV
                     GLLLNFAEGGVEVRRKVRELTSPSQS"
     gene            20164..21639
                     /gene="pseG"
                     /locus_tag="PK634_00525"
     CDS             20164..21639
                     /gene="pseG"
                     /locus_tag="PK634_00525"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HOW96249.1"
                     /translation="MLIRADASPQIGAGHVMRCLSLAQAARAAAWDVVFLSHGDHEPV
                     RRRVEAAGCRWQSLERVQPHPGDLKETLAAAREAAASWVVADGYIFDEAYQAALRDAG
                     FRLMAVDDHRHLERYHADVLFNQNIHAERLRYAADPGTVFLLGPRYAMLNRAFLPWRD
                     RPREIPAMARRALVSLGGADPDNVTARVVEALRGLDVEARVIVGGLNRHRDQLRAQLP
                     DNAELLVDVTDMPAQMAWADLAILGAGSTCWEAAFLGLPVLMIVLADNQRRIAAGLDE
                     EGIGVSLGEHADLKSRMIAERMGEMMPDAGRRRAMSAAGRALVDGGGAARVLAALQSA
                     LVRLRPAVESDARALWEWANDPAVRSAAFHPEPIPWEDHVRWFEAKRRSGRTAIYMAA
                     DEEGQALGQIRFDGTDGSEAEVDVSVASSRRGEGWGPALIRAGCDRLFADRTAAAVRA
                     LVKPENEPSLRAFAGADFDPEDSVAVAGRPVRCFIRRRPHA"
     gene            21632..22327
                     /locus_tag="PK634_00530"
     CDS             21632..22327
                     /locus_tag="PK634_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017712624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOW96250.1"
                     /translation="MPDKTYVVAGCKSWNRLVFDETIRRGPGAWRYVGDPAELTLERL
                     APLAPRYIFFLHWSWKVPEEIVSRYECVCFHMTDVPYGRGGSPLQNLIVRGHSSTKLA
                     ALRMEGSFDSGPVYLKKDLSLEGSVAEILVRATHLSAGMIREIVATEPAPRPQEGTPV
                     VFKRRKPAESRIPRVDSLGQLYDFVRMLDGEGYPPAFLEHEGFRYEFRRAALRGAGLE
                     AEVRVTPIQEDKP"
     gene            22324..23748
                     /locus_tag="PK634_00535"
     CDS             22324..23748
                     /locus_tag="PK634_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOW96251.1"
                     /translation="MNALVIAAHPDDEVLGCGGTIARLAGAGHDVYILILGEGITSRY
                     DCREAADPADLEALREKCRASARRLGARDVFLESLPDNRFDTVPLLDVIKKIEKRIEQ
                     LKPEVVYTQHGGDLNIDHVTTFRATLTATRPMPGGCVRAVYAYEVASSTEWAFQQFEP
                     VFHPQVFVDISGTLERKIEAMNLYESEARPYPHPRSAEILRAIAQRWGSVSGLPAAEA
                     FECVWSIEQDASAQASACTGCAAAAAQNGPPAPHAAGAGLKVDVVCANCEHPVWVWLG
                     DWRARQGAGCSVRLLNCAAYADGGDFLFLISCGEIVRTPILSKYRHALVIHASDLPEG
                     RGWSPHIWQILAGRTRFTVSLFEAADKLDAGPIWKKEVVELEGHELYDEINRKLFDVE
                     LRLMDYALAHADTVKPQPQEGTGMQYYRKRKPEDSRLDPGKSLADQFDLLRVADPHRF
                     PVFFDFRGQRYIVQIRKDKPETGA"
     gene            23745..24797
                     /gene="pseI"
                     /locus_tag="PK634_00540"
     CDS             23745..24797
                     /gene="pseI"
                     /locus_tag="PK634_00540"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HOW96252.1"
                     /translation="MSAAFAIAGREIGPGRPVYLVAEMSANHGQDFDVAVRILRAARE
                     AGADAVKIQTYTPDTMTLDCDRPEFRIGKGTLWEGKTLYRLYGEAFTPWEWQPKLKAE
                     AESLGLQFFSTPFDDTAVDFLEGLGVPAHKIASFELVDTRLLRKVARTGKPVILSTGM
                     ASREEIDEAVATLRAAGCAQLALLKCTSAYPARPEEMNLRTIPDLARRHGVPVGLSDH
                     TLGISAAVAAVALGACVIEKHFTLRRADGGPDSAFSLELHEFKAMAQAVREAEKALGA
                     VSYEIAEHEKASRVFRRSLFVVADMAAGAAFTERNVRSIRPGDGLPPKHLEQVLGRKA
                     ARAIARGTPLAWDLVS"
     gene            24831..25982
                     /locus_tag="PK634_00545"
     CDS             24831..25982
                     /locus_tag="PK634_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOW96253.1"
                     /translation="MLRYCTKCVMPDTKPDLHFDEEGVCNACRNYERRKHIDWKKRRA
                     ELDEVLERFRSKDGERWDCIVPVSGGKDSTFQVLRLLELAMNPLCVTATTCDLSDLGR
                     QNIENIKRLGVDYVEFSPNKVVRRRLNRIGLTQVGDISWPEHVGIFTIPVRMAVRFQV
                     PLLVWGENPQHEYGGPTPAVENRALDRRWLEEFGGLLGLRVSDLAGMEGIEPKHLIPY
                     TYPPDEALKALGLTGIFLGYYMPWDGYTNALLAQANGFTTFHTTVEGSCVNYENLDNH
                     QTGVHDYFKFLKFGFSRASDIASLHLRRGRITREDAMSIVRRLDGRFPWSYLGKSLAD
                     ILNPLDMGVDEFIRICDRFTNKKLFKTDARGQLVKDRFGNLEKVNYDNV"
     gene            26032..26940
                     /locus_tag="PK634_00550"
     CDS             26032..26940
                     /locus_tag="PK634_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96254.1"
                     /translation="MCNLACRHCPTGTVRMPRGLMKGPVFEQVLQAIRRNRGRVKVVV
                     LYHGGEPLLNPRFFDMVGRLKAADPALHVKTVTNGALLSPRAIRKLLQCGLDAIEISL
                     DGASPEENQYIRRKSNTREIVSRIRQLAEMVKRSDSKKPDVYINTTQFIRGHRPPPAR
                     PARPPAWLTRRLAGTVTGIKTAFAIQWPGTSAGGEYEVIPAARRKKAARRCDHVMNTV
                     TVCADGSVAPCCYDLTRRMILGHVLKQPLEKIWNNRAYRALRKAIRTGRPHALCAGCA
                     VVGAAGYLLPLKGPKTVAADEPRPGH"
     gene            26918..27538
                     /gene="hisH"
                     /locus_tag="PK634_00555"
     CDS             26918..27538
                     /gene="hisH"
                     /locus_tag="PK634_00555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOW96255.1"
                     /translation="MSRVLVIDYGFCNLDSVCRAVQECGGDPLLTDDPGQAGSAERMI
                     LPGVGNFAEAMKVIRRRGWDRAIRREVLENGIPLLGICLGMQLLADRGTEGGDSEGLG
                     LVPGRVIRLPKVRPDLRIPHVGWNEVAQTRSSPLFEGVPDRKDFYFVHAYHFEVAEPS
                     DVLGVTTHGPDFASIVGRGRVVGTQFHPEKSLRYGLALLRNFLSAF"
     gene            27541..28299
                     /locus_tag="PK634_00560"
     CDS             27541..28299
                     /locus_tag="PK634_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOW96256.1"
                     /translation="MLKVRIIPTLLYKDHGLVKGIGFDSWRRVGTALPAIKVYAARQV
                     DELIFVDIAATRENRAVDFEEIDQLADECFMPLTVGGGVRAVEDVRRLLRAGADKIAI
                     NTAAVENPELIRGAAALAGSQCVVVSIDVKRHPDGRGEVWTHAGSRPAGREAVAWARE
                     VEARGAGEILLTSIDRDGSMGGYDLDLIRDVAGAVSIPVIASGGAGNYEHMRAAVQEG
                     GASAVAAASIFHFTQQTPLEAKAYLRQQGIPVRL"
     gene            28296..30545
                     /locus_tag="PK634_00565"
     CDS             28296..30545
                     /locus_tag="PK634_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96257.1"
                     /translation="MNELSATTLTFIAERRQWAAAREQGLPEAADVVVTASPEIYFEC
                     RLQGRPCLSLWDYLPVAESVRHEQWVWDWCGRIGRALREREGDGGLSGLIAYDAFHAL
                     VAVLNLACSASRLLAERRPARVRVFSEMQQPLLWDASTPDADLFNFVVDRLARGGGRE
                     MLFLSLTPAVREEIRLADVPPCIPEPDAEAALPSDPVRALVFTQSISWAEQEPLLPVL
                     HREWGGGLVTVMPEPADIRGPYLSWSHLRRLPIRPPPMPDEPAMQAALADAVSDPALP
                     EYVRILAGPECAAFFLRGWRDWLALASAERGIVRLCHRAYRPGVAVLPYDIEGTVRSM
                     AFEWAGAGVPTLAVDHVGLALPCVGPRSLGAGNAIAVWGGPDAALHSRWRDPSARVFQ
                     TGSMRRDLAAAAERGRMRAAAPASPDRPAVLIITAQYGSELMVNGGYPAPGEALSWWT
                     ELARLSVRNLNWDWKLKKHPRFDHESFYRYLAATVENRFEPATPDLLEALDSADVMLL
                     MANASTSAAHAILAGVPVVYFKPPVTPEFWRSPLEDGGAVVVSDMPSLEAALQRMLAD
                     AAYRREVAAAQRAVLDQALVATGDASVNLVRNAIEELRRDGPAPAAPDPAARWIADMT
                     MTVDHGLRGAWPWRAFRRRLRQLAARGRKLAFDDLDFLDADRLPDYFMNIALLSIWRR
                     PEADRRISRRRPWLGRILWEIRRALPDSIRPPAGWLRPYLRRTVEEEAALNPASRFWN
                     ALRRRTAAA"
     gene            30542..32020
                     /locus_tag="PK634_00570"
     CDS             30542..32020
                     /locus_tag="PK634_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96258.1"
                     /translation="MKIMLVNPIIRPDQPPGDFPVGLGIIAEIMRREGHDVRVYDENA
                     LRPDRETFQRDLAAMDEPDVVGVGGLITIYGHLKGLLPALKDRFPRATLVLGGGVTIE
                     PDVIFENMPVDYCVHGEGEHTFRELCAALDASAAKDLPGIKGISFRDRVNGGLVVTPP
                     RPIERNLDLFPMPAYDLFPAETYFRNNRGEVAFWSKNADVQRCATLVWSRGCPNECSF
                     CWRMMGRTVRFRSMDKVMEEIAHLRSRYGVDSYLFVDECINAARVKSVEFAERLVREG
                     YAAPWYSHARADRFDPELAALFRRSGCVGLNFGIESGNEIILATMKKRASPEKAAAAI
                     AIAREAGIGAQCTFIIGTPGESRATVRDSVRWIRKNKVRHFYFFYFTPYPGCEEYHRP
                     ETQERIRRRYGSKDAYFSVLGDAYKLVVNLTGVSDARLTKWREWAFRAARESAWERGK
                     RRVASLASLAGKALKLLVQPSRWRAALGNRLRRGGWYEFRSP"
     gene            32120..33229
                     /locus_tag="PK634_00575"
     CDS             32120..33229
                     /locus_tag="PK634_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96259.1"
                     /translation="MSNTAPKLLILCHHPEPKAGPCMVTLRTVFLRGAPSCAASGRPD
                     IAYAHLDGLRTLPGIVRALRADPDTILLFSSVNALRKPPALWALALNVWLLKRKQVVF
                     WHEASSVVRVTAGLEGPSWWRRMFRRLHFAAVRHFLHNGLTWHLAVSMQTKQLVLFLL
                     GVQPERVFVSGNAIDVERSPVRDRAPARAELRACMAGDPSWQKGLDIFIELAREFPAW
                     RGQPVRYTWYGGTPEWLRQIEADARGEQIRRAGVRLAGFVSPLPDALKDEDLFVFTSR
                     SDGFGLAALEALACDIPVFCFDSAGIAEILPPEFVCSCPADLKKKIEAYLKRRDRYAP
                     GAFRNIALGCGRERFLETWRMLERAIGLTGGLPSP"
     gene            33263..34198
                     /locus_tag="PK634_00580"
     CDS             33263..34198
                     /locus_tag="PK634_00580"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOW96260.1"
                     /translation="MKILVAGGDGFLGSHMVDRLVEDGHEVAVLDRFPDNRTRHLGHL
                     EGRVRLVPGDRAARETAAEALKGQDAVYDFVCATNPAVSWNDIPLEIEQNLLPAVQFF
                     EMAAEAGVRKLVFVSSGGTVYGPAEGAADEARVPLPVNPYGIVKLACEHFLRHVRAKR
                     GLAYDVYRVANAYGPRQSVTSAQGVVAVWMRRILDGEVLQVRGDERTVRDYVFVKDIA
                     RLMTHSLRDLSSSDTFNLGTGKGTSIVDLLEIFRRVVDVPFRFERTPRGPSDATVSTL
                     DSRKLLQHYPGFRFSDLEDKVRETWQDFRARHRCA"
     gene            34227..35072
                     /locus_tag="PK634_00585"
     CDS             34227..35072
                     /locus_tag="PK634_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOW96261.1"
                     /translation="MSLFVIAEIGINHNGDLKIARQLIDVAKDCGCDAVKFQKRAIDL
                     VYTKEFLAAPRESPWGATQRAQKEGLEFGAAQYREIDAYCQEKGIEWFASAWDLESQK
                     FLRPFNLKHNKVASALIVWEDFLKEVAGEGRYTFISTGMSELRHIDRAVEIFRAAGCP
                     FELMHCVSTYPVEDEKTNLRGIQTLRDRYGCKVGYSGHEVGLAVSYAAVALGATSLER
                     HITLDRAMYGSDQAASVEPSGLRMLVGAVRKIEKAIGDGAIGLTPAELPQARKLRAHL
                     PVRWE"
     gene            35092..35811
                     /locus_tag="PK634_00590"
     CDS             35092..35811
                     /locus_tag="PK634_00590"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOW96262.1"
                     /translation="MSTIAIIPARGGSKAIPKKNIMDFCGHPLLAWTVAAARGCPAID
                     GVYVSTDCPEISAVARRYGAGVIRRPDDISGDRATSESALVHACRELEKGGNLPDRVV
                     FLQATSPVRESAELNGALEHFDRAGLDSLFAASVPEDLLVWWEEAGAMKSLNYDFRSR
                     KRRQDAEGGGRLLIETGSFYITRTPILLETGNRLGGRIGAWIVPVWKSWEIDSADGLA
                     LVEAMMKIHRLDRNPPVPVNG"
     gene            35856..36851
                     /locus_tag="PK634_00595"
     CDS             35856..36851
                     /locus_tag="PK634_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOW96263.1"
                     /translation="MTQKAQDRVVYFNGEFVPESEARISIYDSALMFGDMVFEMTRSF
                     GKKQFKLREHLERLMAGVKILHIPLKQTIEDLERACLETIERNEPAFAADDEHRLMID
                     VSRGLLGIYQGVVGLHKGPNVVIADFPLRWTVAGMGKLFEAGINAAVTSQRAIPAFLL
                     DPKIKNRSRIHYLMANIEASKLEGENNWALLLDPDGFIAEGTGDNFFIVKGKTLITPE
                     GRNILRGISRAYVVQELAPQLGLQAVEKNIEPYDVWTADEAFMTGTPFCLIPVTSLNR
                     VAIGTGKVGPVARSLLEAWSRNVGVDVVRQIRDWDRQRTADASGAPTPYSFKGKK"
     gene            36861..37637
                     /locus_tag="PK634_00600"
     CDS             36861..37637
                     /locus_tag="PK634_00600"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOW96264.1"
                     /translation="MKYLDSLFSLKGRGALVTGAARGNGLAIAEALLKAGAAVVLADV
                     LAPELGRVVARLKRAGLKAHARAADVTNPDERAELVRFAERATGHVDILVNNAGITLP
                     HAAMDYPEAHWERTYQVNLKAPFELSRLAARAMARRRRGVILNITSLNAELAFPDNPA
                     YMACKGALKQLTKSMALDFGARGIRVNSIGPGYFRTAMTNGSWGDPARRAARAARTAL
                     GRWGRPEDLAGAVIFLASDAASYVTGQDLYVDGGWLIKGL"
     gene            37654..38151
                     /locus_tag="PK634_00605"
     CDS             37654..38151
                     /locus_tag="PK634_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOW96265.1"
                     /translation="MRASEKVTAKEWAAVKLLALDFDGVMTDNRVLVDQDGKEAVLCH
                     RGDGMGVGLLKKEGVGVIVISMEPNPVVSARCRKLKIDCIQNCDKKLKALQQVAAARG
                     LKPREIAFVGNDINDLECLRWVGLPVAVADAEPEVLEAAAYVTKRPGGFGAVREVAER
                     ILRAG"
     gene            38165..39556
                     /locus_tag="PK634_00610"
     CDS             38165..39556
                     /locus_tag="PK634_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96266.1"
                     /translation="MDAAPRLIHMMFFPWGKDQKLRADQETFDRKPFESMARYAPDFE
                     VRLWTYARAREFCAANYPAIWEGIHRLARPIMMLDVLRWLVVYHFGGVFWQYYQVPLA
                     AMDRMLPSPGRQAKVFTEFVLSPEECRRLAAEPIRNGEPEESIRVLNQVFSAVPRHPF
                     IKATLDLIVERTSTLTPKRDYDILFISANAAVSTAYDRFGKNDPTVELVSRPDTRAMF
                     KTQYLGSWRTDDAAKRPAGPTAVSAVARLARAARAAVKSVPCLAGAYYRWVEPHPHET
                     AFAERPPARRDDGGGEWAGAIPALAAEFGIRSVLEYPCGPAAASRLAAWNGLTYIGGD
                     PVRETVKRIGPAGAGAVRFMNAMYSRLPRADLVVCRNFFVRLPYRDALAALKRLRASG
                     ARYLLATTFPLLNANWDGALGDERPLNMTLLPFAFPEPLRVLPDPCPDGRTDRSLGLW
                     TLADTRQSTIEHT"
     gene            39631..40788
                     /locus_tag="PK634_00615"
     CDS             39631..40788
                     /locus_tag="PK634_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HOW96267.1"
                     /translation="MKRLVFAPGLGQLVNAAAALTQDRVRDRGSTEEHEDVLVFFANG
                     RYTDLARAMQEAARCLWSWRAIEWAEDVLVSHFPTRRFAPIARDVLRERLGADPDEIW
                     VSKLGLEATKLILYAFPGARVVLFEDGAEEFIPQEVLCGRARLKQLKPAGWPGGLRRE
                     WSHWGRTSECLGMEGLCVRDRARVVRMYSYLARQVELPAYLSGVPVVHVESESLRGRL
                     QALAPLFDPAQCRQPPKRPGESAVLFLPQPFADIFLTAENEYALYRAAVSEILDKGYS
                     ILWKEHPREITPLAPRLKQDLAGDRLQILRVRQQIPVECLVAGWDLAAVVSVSSTSLL
                     LLGGLYGYPAFTAADRISIDCWRKRSDSELARLFLRTLPGLDQLPAARAQE"
     gene            40815..41954
                     /locus_tag="PK634_00620"
     CDS             40815..41954
                     /locus_tag="PK634_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOW96268.1"
                     /translation="MTATLKEAVAAAPMYRTLFPEGWNREKPLHVFCRADDVRTIRRL
                     FKARHGLRVSRFILPDDFWQSRYRAKMLLRSALRGLGIRLCLLDDLDPASEPALVAYA
                     PPGYEVWQSQRLAQGGFRRVMLFDDPAYDERFRRETDFLEKYSAPLHALFERLQDEDS
                     ALTLASVVRHRQTGSFLFLRLASYPQYGHPVVRAAPGDTILDVGAEDGGTSIMYSNQV
                     GASGRVFAFEPDPMNLETLRGLFIGRSNITTVPMALGETEAEVRFVSQKGGLSHIARP
                     GETGGIPVRVAPVDKFVEENKLARVDLLKLDTEGYEMPILQGAVETIRRFKPRLQISV
                     YHKQQGRDDLADVPALISRVQPSYRYYLGHHGSWGNETILYAICP"
     gene            41951..43393
                     /locus_tag="PK634_00625"
     CDS             41951..43393
                     /locus_tag="PK634_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOW96269.1"
                     /translation="MTRASLSFILRRAEGVAFAALLVLLAPALLVAAIVRLARGREAA
                     RRLPAMWRASFRRWGKFVAHGLRPVREAWWPAMSYEPPLNVWRYVFLLELFDVVNVAA
                     ADRPAAEPGDRARVLVVKLAHVGDALHIGPSLRALREQRPGWALDLVAGPWCDALVRK
                     IPYVAESVPYAPRLVLFNRGRGDRGLSLAGEAAFFWRLRRRRYDWLFSTSVTSLVEVC
                     MIQAVRPARWVGAAHDAGLWHPFSEARTQPYTSRKYEAQRIADLLPLIGVQAPRAELE
                     YWLSDGERAEAARRLAEAGLRAPVVAVAPGAGWPGKLWPVERFAELADRLAERQKASI
                     LILGSPGERELAEQMKSRMRQPALNLAGQTTLDQMAALLAESDLLIGNDSAPAHFAVA
                     AGTPALCLFGPTSRPQWGPPEGGPHRVLQAPKLCFDCHPFHPRAACHDDRSCMKSLSV
                     DAAADAAEAMLRDTSHVRNRGIHDVSVRSG"
     gene            43371..45236
                     /gene="asnB"
                     /locus_tag="PK634_00630"
     CDS             43371..45236
                     /gene="asnB"
                     /locus_tag="PK634_00630"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOW96270.1"
                     /translation="MFRSGPADPEAVLRDMARTLEKRGPDGEGFHRDGAIALGHRRLS
                     IIDLEGGAQPMSTADGRYTVTYNGEIYNYLELRADLEREGTAFRTASDTEALLQGWAR
                     KGPEALKDFSGMFAFALWDREARTLHLARDRMGIKPLYWTQVAGELVFASELKALLRH
                     PGVARRLDLLSVSKYLTYGYIPAPHTIFEGVHKLEPGTRMEFDGAAPRMASYWDIPLR
                     DRPVSSRSADECAADLRVLLRDAVRQHLRADVPVGVFLSGGLDSSLVTALAAAESSRP
                     IRTFSIGFEEASYDESPFAREVARACGVEHRAEVLSLRRALDLLPGVLDRMDEPLADA
                     SILPTSLLAQFAAREVKVVLGGDGGDELFAGYPAFQAHRLVERLSFLPMAWRDALTRA
                     ARHIPVSHRYASLEFLAQQFFKGAGISPEVRFLVWLGCCGNEQKNGLLSADAREALRR
                     RDPFEDVHRYVRQSGLIGGFERILYLAAKLYLQDDILVKVDRASMAHSLEVRVPYLDP
                     AVVEYAAGLPMEYKLRGFTTKHLLKRAARGLLPERIVQRRKAGFMMPVAAWLQRDLRP
                     RVEELCAPDRLARDALFDPKTVRQLLDEHFSDRRDHRKVIWALFAFQHWRQRYLP"
     gene            45258..46355
                     /gene="wecB"
                     /locus_tag="PK634_00635"
     CDS             45258..46355
                     /gene="wecB"
                     /locus_tag="PK634_00635"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOW96271.1"
                     /translation="MKRKVTCVVGTRPEVIKMAPVILALRGDPSFDATVLATAQHREM
                     LDQALAMFGLRADVDLDLMRPDQAVGELTGRVLAGLGEHLATHRPDAVLAQGDTTTVM
                     ATAMACFFAGIPFGHVEAGLRSFDLQAPWPEEYNRRVAAVGARWHFAPTPGAVKNLRR
                     EGVEADRIFVTGNTIVDALRHILATTPAPPPPVPAGRPFVLMTCHRRESFGEPIRAIF
                     RAVRDFAARHPDAQVWYPVHPNPGVRGPAAEILGGLKNVRLADPVDYVTLLHAMKACR
                     LVLTDSGGMQEEGVTLGKRVLVLREVTERPEAVESGLARLVGNDGARVGAALEQAWTD
                     SAEEAPGSAVFGDGHAADRVAAILRDRKERS"
     gene            46352..48670
                     /locus_tag="PK634_00640"
     CDS             46352..48670
                     /locus_tag="PK634_00640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96272.1"
                     /translation="MTEKKPLISIVLPTYNGAKYLAEAVESCRAQTWREWELIIVDDC
                     STDATPDLIRTLAQKDPRIRSVRHERNRKLPAALNTGFAQARGEFLTWISDDNAYRPE
                     ALAEMAAYLLAHSDVGLVYTDSMVVDENGRPLRVRRVPEPETLLERDPVGWSFLYRRA
                     VREAVGDYAEEFFLSEDYDYWLRVSMRFKLAPLHKDLFLYRQHGSSLTASREKEIERT
                     WPRMLEKHLPHLGWATPGQKAECYYMLAAAARARRDVAAAARCWLGALRNDPAYALRR
                     AGGKVARLFRKPAPPPDYKAAIARAREWIRANSEDGGVYFASCRREVYREVTGYYIPT
                     LLQIGEPQVARRWAEAMLQALGPDGSVSSIDGKLYVFDTAQVMRGFLCFADEEPFREG
                     LRRAGGYMLSQLREDGSFPDVYDGKIPTYIFLYALEPLLEAGRRLDRPDFVEGARRAL
                     DYYERIFDPRRYASNSHFFGYAVEALIDLGRPEKAREAAAYVKTLIRPDGGLPAAAGA
                     RWVCSPGQAQFARIFFKLGDLETGDRLLGWMLKNQNPSGGFFGAYGKDSLYFRDSEPC
                     WAVKFFVDAIVLRIRRHFEVEFPSWMDNKPVAEDFRFKALCGALPESGSRLRVLEIGC
                     GAGRFLQALRDLRPNHEYVGLDLSETLVRLLPEGIRGVTGDMLNLPFGAAEFDAAFMV
                     EALEHAPDVGRAIAELMRVLKPGGRFAIIDKNAACLGRLRIMPWEQWFDAEGLLGRLR
                     AGSDDAKVSLIPAPERFADMFLIWQGTKKPGA"
     gene            48672..49610
                     /locus_tag="PK634_00645"
     CDS             48672..49610
                     /locus_tag="PK634_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96273.1"
                     /translation="MAAPDQPLVTACVGTYNRERYLRETLDGVFAQTHPRMEVIVVDD
                     ASTDGSARLIEAEYGSRLQFVRLPKNSGRPAVPRNVAARLAKGEYVAFLDSDDGWYPE
                     RVARQVAFLEAHPDIPFCHAYCHLLDEDSKVLGVRHEGALPPTGDCFRELARHCFISI
                     SSVLVRRSLLDEVGLFNEDPRYRAREDYEWFMRIARRRPVGLVDAVLARYRRASSGIS
                     QVEGAWRTRPQDAEAHRLLLKRRDIWEGRVPRRHMKDALLNALEENTIWWRDRGRAGR
                     ALWFALWAWRTAPARPAVWREGLKTLGRLIVPRSRT"
     gene            49607..51022
                     /locus_tag="PK634_00650"
     CDS             49607..51022
                     /locus_tag="PK634_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW96274.1"
                     /translation="MTSAHRIEYLVLGAGPTGLGAALRLEEKGLGWRLIEAGPRFGGL
                     ASSWVDDRGFTWDLGGHVQFSHYETFDRYMDLALGPDGWHRHERESWVWLKNRFVPYP
                     FQNNLHRLDPADREDCVEGLRKAAAARTLHPEPCRPRNFGEWIDQTFGEGVARLFMRP
                     YNFKVWATPPEKMDFHWIGERVAVPDLKAVTRSIETGQDQVSWGPNRTFRFPKRGGTG
                     AVWEALGRRLPAERVTLGAEIVRIDPDLRQATAADGRTWLYDRLISTAPLNRLVRMAP
                     GIVDDRVADRLVYSATHVVGVGMDGTTPERLKTKCWIYFPESNSPYYRVTVFTNYSPE
                     NAPRPGAQWSLMTETSESADKPVRRESLAEDTVRALVEDGLLPDRRRVVSVAHRRLPQ
                     AYPTPFLGRDALVDPALRAFEARGIFSRGRFGAWKYEVGNQDHSFAQGYECVERLAAD
                     GGAEYEPTLFTPDLVNNRRNP"
     gene            complement(51031..51993)
                     /locus_tag="PK634_00655"
     CDS             complement(51031..51993)
                     /locus_tag="PK634_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96275.1"
                     /translation="MKKILMVLTSHRLDCFRLCMDLLVRGGSIRFFDRVVLLLNGVAG
                     RHLRYVRDLQAAHPDIPWDTIAGPRGRGRLISNLQNECVRRHPDSLYFKIDEDTFVSA
                     DWPQRLTAAYEAHRDDPGLSLITPVIPNNALGCFYLLHAFPELAREYDRLFRKPLTTK
                     FDGPVWINGRIADWITREFLDLRRANERLRAVRAPAIAGDGLSMPEGGPFGRFAFRFS
                     INCLCYDYRHWRELGEIPEFDEIGWGDWIVAHGKFAVLAPDALVHHYSFFVQQDWLDR
                     TPLLEDLRRANLPDTLRWPDRGPARLGRIVQQVPRAIRRRLGRS"
     gene            complement(51996..53381)
                     /locus_tag="PK634_00660"
     CDS             complement(51996..53381)
                     /locus_tag="PK634_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW96276.1"
                     /translation="MRIVIIGAGPCGLGAAHRLLEKGHSDFAVYERRPYVGGLAASFR
                     DERGFTWDFAVHVAHSHYHYIDRLMDDLLPGGFLHHERRSWVREYQTFIPYPFQYNIR
                     HLPPAARDECLAGLRELAQRPPSDAQRPTPNNFEEWILAGFGRGIAKHFMAPYNRKIW
                     STELRDMGYQWIGDRVPTLDLARVERNIAEGRDDVSWGPNHTFQFPREGGTGAIWRAL
                     AARLPAGRLHLSCGLVRLDAGRRIAVFEDGREERYDALISTMPLIRLAELAGDTGLVR
                     RARGLRYTHVEVTGVAPAFPIPDALRDKTWIYCPEPACDFYRVTPFSIFSPAHVPDPD
                     RHCSLLCEISTPGDAPIPRGDSGAATLRGLRDIGLMDCDPARTHLFTMVEEFGYPVPT
                     VDRDAILADVLPALESQGLYSRGRFGGWKYEVANMDHSVMQGVEVADRLLDGAEEVTL
                     PKPAVVNAGKR"
     gene            53520..54683
                     /locus_tag="PK634_00665"
     CDS             53520..54683
                     /locus_tag="PK634_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96277.1"
                     /translation="MKISFLVPDIYCPVLGPVTVLARTLQRHYPVQVAGPDFGHGVCP
                     MYRGAFDYTVVPTPRLYRLPDCLWESRRLGAALTGDVIVAVKAFADTVPVALREKRRR
                     GAKVVVYLDEWDGALFHQMPRASRWKSVLRNLHHPLEEPWNPLVEKLIPKADLVISTS
                     TWLQNKFGGRIVHMGVDTDFFKPSSPERTAALKKECGLEGLRCIVFGGVVRPHKGIEI
                     ILDALVRLGRADTRFVIVGPVNEHVEKLRSEPSYRPYLATLGPQPAARMPEFLSLADL
                     IVLPLKDTPLARSQMPCKIFEAMAMARPVIGTAISDLPLVLEGCGKVVPPDDAAALAE
                     TMRQALDHPAEAEAMGRAAREKCIRLYSRGATERELVSIMRGFEQEATERTEG"
     gene            54791..55744
                     /locus_tag="PK634_00670"
     CDS             54791..55744
                     /locus_tag="PK634_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOW96278.1"
                     /translation="MIKGKKIFITGGAGFIGSTLIGRLVEQNQIVAYDNLARNALQTQ
                     AYKNHPNLKLVVGDVLDYDSLAKAMADADLVVHCAAIAGIDTVVKSTVKTMRVNMVGS
                     ANVLEAASKLTHCDRVVCFSTSEVFGTNAFRSSELDKTSIGKVGEARWTYAVSKLAEE
                     HLAIAYYQDQWLPVTVVRPFNVYGPGQVGEGAIRIFIQRALKNETIQINGDGTQIRAW
                     CYVDDMVRGTMLCMEHPKAVGESFNIGNQRAVTTIYGLANTVIRVLNSKSKIEFVYKD
                     YADVELRIPNVSKARELLGFEAKVDLEEGIALTAEYYRAQG"
     gene            55769..56644
                     /locus_tag="PK634_00675"
     CDS             55769..56644
                     /locus_tag="PK634_00675"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOW96279.1"
                     /translation="MTEQTTVASSSGWIAGSALDRPGECRLRFHPAGADEGIRFVRSD
                     LPGAPEVLCGPEQLRSMPRWTLLEQAGVAVHHTEHVLAALAFCGVDNARIELDSEGVP
                     MTDSGRCATFHAALSRLGTRALGAPRRVYALAKPLFFLDRQRTTGEESAHPELRDGRY
                     VLGVPADHFSVSYVFHWSHLEGLPIGVAEYDEAAPDAARHVLDARSYLVETETEKVRD
                     LLGPVRETVLMLYPRCPMELGLEAARHKIVDFVGDLRILGRPVRGRFIAFRTGHRIHH
                     ELVGHLLRDKMLQLV"
     gene            56680..57369
                     /locus_tag="PK634_00680"
     CDS             56680..57369
                     /locus_tag="PK634_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HOW96280.1"
                     /translation="MNAEPLSPGLVILGAGFHAKVVIETIRLAGLGPVLAVLDDAPAK
                     HGTAIHGVPVRGALGLLPEWHARGLVLGVALGMGNIRLRDKAAALMRWAAGLGVDAPP
                     IVHPAAFIAPSVARPRGLFAGPGVVVNTDSQLGGNLTIYSGTSLDHDNVIGDDVFISP
                     GVHSAGKVRIGAGAYIGPGAILGSAVRIGAGAIVGAGAVVLKDVPDRCLVVGAPARVA
                     RTVDEWEKAHP"
     gene            57366..58445
                     /locus_tag="PK634_00685"
     CDS             57366..58445
                     /locus_tag="PK634_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOW96281.1"
                     /translation="MIIPTAKPCLDASDRAALLRVFDSGLVADGEEIAAFEAELAARL
                     GLPGAVAVGSGFAALHLALLGLGVGAGDEVVVPCVSTCPAMRNAVWAAGATPVYADVN
                     RADFNLSAESARGVLTPRAKAIIAPHHTGVIADIPALAALGAPVIEDCAQALGGTWQG
                     RPAGSFGAASVFSFYATKIITTVDGGAVASPSAPVLERVRGLRYYRHCGDDRMRFNYK
                     MQNLGAALGRSQLTKLPGFVQRRAALGRRYLAAFCEAGGRESQSLHRNDGSVYFKLAL
                     RLPPATRDRLLGAAERLGLPCSTEFWWIAPSAGERYPNAARLMKRIVALPVYPAATDE
                     EAARVETAFRQALKEAGPLRDGEEA"
     gene            58442..59170
                     /locus_tag="PK634_00690"
     CDS             58442..59170
                     /locus_tag="PK634_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOW96282.1"
                     /translation="MSAPRKLVAIHQPNFFPWLGFFNKIARADCFILLDNVQLPKTGG
                     SWVNRSKILAGPGKDKWLTLPVDRSYHGTRLITETQISMPASERYRLADVVRFNYQKA
                     PAFAEVFPVLEPLLKSPEMNLAEYNILAITTLAQRLGIPGGRFVRSSTLPSAGEVSTE
                     RLVTLVREVGGNAYLYGAVAAATYQENEKFEAAGIAPVAQNFSHPRYAQFNTPEFAAG
                     MSIIDALMNLGFAGTQGLLPGNTA"
     gene            59167..59907
                     /locus_tag="PK634_00695"
     CDS             59167..59907
                     /locus_tag="PK634_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW96283.1"
                     /translation="MTEPTTQTTRPALSIVILAFNEVDNLRPVVEETKRAVAANPWIG
                     TFEIVVVDDGSTDGTAGVADALAAENANVRAIHHPRNLGIGAGTRTGYGAAQGDYVMA
                     VGADGEVEMAEVLQLVKLMDGADLAVSRRRRGPGAHRKLFTYGFHRLTSLIVGFNIEG
                     MEGIYVVRRDILRDMELYSNTSLVNLEVIMQAVRRGCRIRSGEIGIHPRLSGESKMTN
                     WRAIARVFREMLKLRWHLWRAGRRKKGK"
     gene            60051..61040
                     /locus_tag="PK634_00700"
     CDS             60051..61040
                     /locus_tag="PK634_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOW96284.1"
                     /translation="MKTLKQNWRLGLGLLVSALFLFLALRNVHWADVLTAWKGARPSL
                     LAAGGLLLLGGWVVAAVRWRVLLSPAPGLRVRDTFAYICIGFLANTVLPLRLGEIARA
                     TWIGRKKGLGVSRALGSIAVERVFDLLSVIIVTLLLTLLVQIPPIIQAGLASMIAAAL
                     VAFVGLLALALNQERLRRLTGLLARVMPHRIADRVASIASGFASGAAATRRPATLAAA
                     AGLSLAIWTIGGTATLLWVRAFNFDTPWFAGFLVLVVVNLGSAIPSSPGYVGVYHAAA
                     ILALSLWVPKGPALAYALVTHALNMLANVVLGSYFLVREGVSLKGIEEQEVTE"
     gene            61134..62705
                     /locus_tag="PK634_00705"
     CDS             61134..62705
                     /locus_tag="PK634_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96285.1"
                     /translation="MGWGVVLGVLLLQVWSRYLPSPWAVFVADDWTNYARSTLFPSMK
                     EAVTVSLQDPNRPLSTTAVAVGYRLFGPRPVYWTILSLLSHSLMLWFIMKMGLELTGS
                     RRVAALQGALFALLPNLHETWQWSTQILNEVTCGLLFYALAGWLFVAHVRRGGAAWLA
                     GAALAYAVGLFSYEQGILLPAVGLAILPWRRSLGRALLKMVPFGAAFLLYAAWRATDS
                     FGLNTAWHYPDQMKVGFSLWGIGWNARQLVHWWIGDRFFGAILSGLMSFSTLAPWTRR
                     LLALGNIAAISAAVFLWRRLASDKEDALPAAFSAAQVAGFGVLWAGASMATILASYTA
                     GRLNILPAMGLTLLGGLLLARLPWCAWATALFVPALVILAANQGTSENFRQAGEFNWR
                     VYDYLRVRQAEWQDKDVLLFDTGSLRHRQTRGLVTPASALEYTWAEYGNTLLFRGFVP
                     LGMVQRLAGAMSPHVQVIQDVECGARVDGSELAWHTRYRPDEPFRTPLDRVFIVDVFQ
                     AAQSVPPRPPLSPLP"
     gene            62702..63907
                     /locus_tag="PK634_00710"
     CDS             62702..63907
                     /locus_tag="PK634_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96286.1"
                     /translation="MKIAYLCEPQAGGTFTFFQRVRPRLAALGVDFRCVPPYSVETFA
                     GSRFEGLEGVDFLELPDGLPDATRALVRHLELQDYRGALILPGCDVLTTNLARYLPRG
                     VRCAARVALMARSAYVSARAVAGHLNAVFAVSHRVAHDLEHSYGFPAGSVRIAYNGAV
                     LRPLPARDPGRRPFRLIYTGRLSDLDKGTLMLPAILRQALDAGADVFLTAVGNGPDEQ
                     RLREGFRRLGLEDRVELLGNQPLDRVDRLLDESDGFLLPSRLEACPNAMLQAMAAGCA
                     PVAFRIHDSVDRIVEDGVSGLLAPVGDVAAFAQAVVKLAGDRALCARMSAAARKRIEE
                     HFSAERTAQLYAEGFRAMLDGPDRRAPALSLDRYEVPRELKPTWRTLIPAPVKNMLRK
                     WMERFGRTA"
     gene            63987..65177
                     /locus_tag="PK634_00715"
     CDS             63987..65177
                     /locus_tag="PK634_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96287.1"
                     /translation="MKVLFINRMLSLVRGGGETFDLEIGRHLAKLGCEVSYLSGLPLF
                     SRGRPPVIPASFQPPPAMHTVRSPWLAWIPWDRMRGGWRVWLLDRRLFEARALKWVLP
                     RQAEYDIVHLCEMPDWPAAAKRAGLKIPVVVRLTGPEGAKDSPGLRATDGVIASGVSV
                     EKIRRLVRPDCENIPNSVDTDLFRPQPSDFRRRLGIGPDTCLVLYVARFQDFKNHQML
                     VRAFAEFLKERPDARLILVGEGHLKSWVQKDCAELGIADRVLFLGEVPFTELPALYAA
                     VDIKVISSDYESFSFATIEAMASRLPIATTDCAWVPVLIGDSSSSSTLHPSSLRLAPG
                     GVISPIKDPGALAQGLLRLAGNPELRKQMGEWNRRKAVAEHGWAASAAKLLALYRRLT
                     GKES"
     gene            65179..65694
                     /locus_tag="PK634_00720"
     CDS             65179..65694
                     /locus_tag="PK634_00720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOW96288.1"
                     /translation="MNGIRGLLKRFWSRRQAEVQKKWKRTLPFGDYVVDRWEKARLLG
                     FGEGASIYDSAIVMGDVQVGARTWIGPGVILDGSGGLAIGDYCSISAGVQIYTHDSVD
                     WAVSGGQKEIVRQPTRIGSRCYLGPNVVVAKGVTIGDGCIIGANSLVLHDIPAGHKAY
                     GTPCKVVGPVG"
     gene            65716..67284
                     /locus_tag="PK634_00725"
     CDS             65716..67284
                     /locus_tag="PK634_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96289.1"
                     /translation="MNEALLLLLVLAAVLTARLWPSWRHPQIGSDAGFHLLLRREIRR
                     GGFRPPARLEACLFDRTVGYPWLYHQFIALFPESWLRRLPALPSALADAAQTGLVFLA
                     GRALAGRFAPGADPAWTGFVCAALYGLNPAMLAHGVGPRAFEITPRPFGELLFTISLG
                     SALLAAYLSSWAWAAVSVLAGAGVLLSSKFAVQVMLFFMPLLSFLPGLHRLWLVPLAS
                     LPVALALSAGRYRHVLRGQVRHLDYFRRKLQYTFGTVANRNRWQDVRLLAATVRGHGL
                     FSRAAASAASDFYFHNTYLQMATRGVLFLLLAVLCAGGSFRGILLYRWLLGWAWVWVL
                     PFLLTSWRHARFLGEAERYAGYSIAPAALLVGLGLVSRPVSGLLAAGLVLYALVTIVI
                     FAHALRSHARSAGLVRDARRELAGFLGGLPAGAVLLGAPEELVLGPVASLLPHRFADI
                     MADMDLFVEKLEHHCDGYPWPRADWAFWRAAGVQYVVARHPDEIRAVRPDLKYDFASL
                     ERLFSNDAYDVYRL"
     gene            67289..69160
                     /gene="asnB"
                     /locus_tag="PK634_00730"
     CDS             67289..69160
                     /gene="asnB"
                     /locus_tag="PK634_00730"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOW96290.1"
                     /translation="MCGIAGFIGEGLGLTPERLARMRDAIAHRGPDDHGLKAWTAAGE
                     DAAPDAVAAAGLAHRRLSIIDLSPAGRQPMSNEDGTAWIVFNGEFYNFGDYRAELEAQ
                     GHVFRSHSDTETILHLYEQHGIEETLRRINGMFALALWDARRCRLILARDRLGKKPLF
                     YAWQPGQGLAFASEIKALRAGGLVDDARLDLVALDQVWTFGVTVGERTLFEQVRKLPP
                     ARYAVFENGKLEQREYWDAVFRGAPADNRADGDWVDELDALLRDAIRLRLVSDVPVGL
                     FLSGGVDSSLIAALAADITGGRIHSYTIGFREDAFNEAPLARAVAEHLGLKNETLCVT
                     DDLFGEAGRIARQFDEPFGDSSAVPTYYVSKLSRKFVTVALTGDGGDESFAGYDYIRQ
                     AARMWGSAEHRRRLRRPMGLRERLWEFKQRLLGFERGFLNLDSQLSRKRRLELFTPEF
                     LRGVPHGEAVADRARWMARTAGADVLSRVQYLLVKTWLPDDFLRKVDTVSMAHALECR
                     SPLLDYRVVEFAARLPDRLKFEPEGRGKRILRLLLQRYVPEELYDRPKRGFGIPWEHW
                     CRGEAGRRLKARWEQWANPWFRPEAAAMLFPEDRVGVNFLQWTAFATLEHFRPEAGA"
     gene            69157..69861
                     /locus_tag="PK634_00735"
     CDS             69157..69861
                     /locus_tag="PK634_00735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOW96291.1"
                     /translation="MKQGIKEWIAGSGLRRLVNTWLGGNRYAVRGSGNRLDCSRGLLT
                     RVRVCIGGNDNRIEVGEGARLANLDILVRGDGHRLSIGAGCSIAAGKIKLEDRGSAME
                     IGAGTTIEDAYLGAYEGARVVLGADCMLSEAVGLRSGDMHSVLDAATGARLNPAKDIR
                     LGDHVWLGRGVTVLKGAAIGDRAVVGAQALVTGDIPAGSLAVGAPARVIRSGVTWDRR
                     RLAVTEERKKEEIDRR"
     gene            69917..71320
                     /locus_tag="PK634_00740"
     CDS             69917..71320
                     /locus_tag="PK634_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOW96292.1"
                     /translation="MLFNSYEFLFLFLPAVLLGYYVLLPKRARLLFLTVTSYLFYGWW
                     DYRFCALMLLSTTIDYAAGRGIGAAKTPRVRRRWLVASIVSGLSLLGFFKYFDLAART
                     ANHVASFLGAPEPILPLLHIVLPVGISFYTFQSMSYAIDIYLGMARPARTFVDFACFV
                     ALYPQLIAGPIVRYRDLAEQLIERTHTLAKFSLGVAFFVLGLAKKVLLADGVATLVAP
                     AFEQAAPGLFTAWTGMLGYAMQIYFDFSGYSDMAVGLGLMLGFRMPQNFNSPYQAVSI
                     TDFWRRWHITLSSWLRDYLYIPLGGNRLGPVRTYINLFLTMLLGGLWHGANWTFVVWG
                     GYHGILLAVERAAGKKGLLSWAPPAVQRALTFLLVLLGWVFFRATSMGEAGAMFKGLF
                     GVHGLGHGIRAALGAAPLPYLMLAVTMVLAFGFRNTWEIRWRNGWRLALALAVLYAVC
                     VGAILVNTSSPFLYFQF"
     gene            71334..72617
                     /locus_tag="PK634_00745"
     CDS             71334..72617
                     /locus_tag="PK634_00745"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96293.1"
                     /translation="METLNDKEQTWAEGRSWRGAVLAVVGLAWLAGLGFDWMLRFTWY
                     GWQQRLIFRPSAVRTLNQSSDRYVTNEVPGIRGGDLTRLVGIRSFNARFEQDHPGGPQ
                     TCDGAGFRNLPAPPDLAHPVVVVGDSFMAAGDPMTNMFAVQLAERSVLPVYNRAMVGV
                     GPFLSVERFVDSDRFQHRRPKILVWGFVERSVIQHGFSSLLYQLDIRDKGLKPAENYI
                     LSATTTRVVWQALLPGNLKTALPDTSALAQLSQRAWNRIRYALFGKIMPEVFAAAGDV
                     AGKPILFYRPALDLSLGDPFERDPDQIMEGIRYTSDFMARRGIRLVILMIPDKETTYR
                     ERIPPAELGGRELPPSCLLEIERRLRAENIPVVNLVEPFRERAARGELLYWPDDTHWN
                     SAGIRIAAELVWRELQSLPETQALLKHEPPSDPLQ"
     gene            72589..73731
                     /gene="rffA"
                     /locus_tag="PK634_00750"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             72589..73731
                     /gene="rffA"
                     /locus_tag="PK634_00750"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015211512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOW96294.1"
                     /translation="MSPHRIHFNSPRAAGRELANMARALESGHISGDGEFTRKCRDLL
                     KQELGATDVLLTTSCTHALEMTALLLELKPGDEVIVPSFSFVSTANAFALHGARPVFA
                     DVRPDTLNLDERRLEELITPRARAVVVVHYAGVGCEMDAILAAARARGVAVIEDNAHG
                     LFGKYRGRWLGTFGAAATQSFHETKNFTCGEGGALLVNEPRWRARAEILREKGTDRGR
                     FFRGEVDKYSWVDIGSSYLPSDLLAAFLLAQLEQRAAIQAKRKALWHGYRDGLADWAA
                     ARGAQLPVIPAHCESAYALFHLLLPSAEERDALMAHLKARGISSVFHYLPLHLSKMGR
                     TFGGQPGQCPVTESVCDRLLRLPFHNGLAEANQAEVIEAMRAFRPG"
     gene            complement(73718..74737)
                     /locus_tag="PK634_00755"
     CDS             complement(73718..74737)
                     /locus_tag="PK634_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96295.1"
                     /translation="MGRRLSIIIVAFNEAPNLPRLQPSIARLKRPAGCEIETVLVDGG
                     SRDGTAQAARERGFDRVLDAPGASIPVCRNIGLKESSGEWIAFLDADCEPAADWAEQA
                     AAQLEHEPRVILGWPVEPPAPMTWVQAAWHFHWQHKNRAIEQSARGEVVRREAFRLVT
                     TRNMWLTRSAAEALGGFDERLATGEDTDFAFRAHRAGIPVLGVPALRVVHHGEPATLR
                     AFFRQQLWHANRLSYSRVDGSGQRAGGNAPRFARLFAASLLLFALAVPAGWLGHWSGW
                     LLALPLPVLVAGPAAWTCRKGGSFRHFPALCVLYFAYGLARAIDLAGLHREKKSWKSG
                     SVSRA"
     gene            74789..75352
                     /locus_tag="PK634_00760"
     CDS             74789..75352
                     /locus_tag="PK634_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOW96296.1"
                     /translation="MAGMKHVGMVLAAGASSRMGRPKALLPAEGGRPLACVQAGLLRA
                     GGCARVVVVLGADFDRIAPQLAGEEVVRNPEWEKGRFTSVQTGLRALGDSDGCVILPV
                     DAVGLRVETVRAVLARAETGIAPAVRPTWRGQDGKLLWISRALAEEWRREPAGDQRLD
                     DRVRARVERVAVDDPALLSNVNTPEDL"
     gene            75412..75813
                     /gene="mscL"
                     /locus_tag="PK634_00765"
     CDS             75412..75813
                     /gene="mscL"
                     /locus_tag="PK634_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020193520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOW96297.1"
                     /translation="MGMIKEFKEFAVKGNMIDMAVGIIIGGAFGKIVSSLVNDILMPP
                     LAMLMGGINFSNKVITLKEAVGEAPAVQLKYGLFINTAIDVVIVALAVFMMVKAINRL
                     KRKEEAKPAPAPAISDEVKLLTEIRDALKPR"
     gene            75830..76450
                     /locus_tag="PK634_00770"
     CDS             75830..76450
                     /locus_tag="PK634_00770"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOW96298.1"
                     /translation="MNPVEAGPASNRIRWMANAAVFVSGACIMIVELLAGRVISRHVG
                     QSLYTWTAIIGVVLAGILAGNYAGGMLADRRPPRRLLALLFLLAAAGCFAVRGLNDLA
                     GHWSLLRGQAWPARIVLHVLITFLLPALLLGTIGPVAARMAVGADPRTGRAVGDVYAW
                     GSLGAILGTFLAGFVLIPSFGVTAIVGGVSGLLAVTGLLYAAASLH"
     gene            76457..77452
                     /locus_tag="PK634_00775"
     CDS             76457..77452
                     /locus_tag="PK634_00775"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOW96299.1"
                     /translation="MKIAVIGDGGWGTALALILAGNGHEVSVWGPFEDYLRTIRETGE
                     NKKFLPGVRLPSALRWTARLDEAAAGAEAVVLATPSRFFRGAVSALAPFLPNPRPLIV
                     SVTKGLDEKTHRRMTEVAGELLGGEAVAALSGPSLAAEVARGVPTAVTIACADMGRAA
                     ALQLLFNGPRFRVYTSDDVVGVELGGALKNVIAIAAGVCDGIGFGDNAKAALITRGLA
                     EIGRLGVAMGAHPQTFAGLSGLGDLVVTCASRLSRNRGVGERLGRGESMEAILQGMQQ
                     VAEGVWTCGAARDLARSRNVEVPITEEVCAVVHEGKNPRRAVEDLMSRDPRPERE"
     gene            77839..81480
                     /locus_tag="PK634_00780"
     CDS             77839..81480
                     /locus_tag="PK634_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96300.1"
                     /translation="MYANPPESNSYANATRYFSGGALTEGQVFSIDLAVNHWNGNKGI
                     DIYSAGGAKLLNINFSDNNGGLKVEYGITTNILGWSNSGAGYNYTLEVEKKAGTTSRV
                     SLQRNVDGSNYVAELDLGNAPGGFHLYSGATEAGAPNDLFLNNMSLADSGTPVLTLVG
                     RAAMPANFTNRLTVTRNGSTAAALDVTAWSGDEAVATVDTNVTIEIGSNSAPVEIVGV
                     DTGTALIQATADGFPTASLSVAVFDVAYDDSSYYPSGGWTSGANQGSGFAAWIITNNN
                     GEGVGFTNYAGSFIGNSTADGGGHVNAQPTGDAFGLYANQNGTGGDPLSQATRPFAAE
                     LAAGDTLSVDLGVNFRDGSKGVMIQSGDTWLLEVAVIGDDYVYQNHGGGGAQTSLGWT
                     YEANTSIRVEVTRKTSFLYDVSIIRGGTQTATNKLAGVNLGGAPDRVRFYNFNTAGGG
                     GNNLFFNRLAIWSGAETPVLSLAGQEGMVVNHTNKLVISRTGEIGEVLDVNLASDDTN
                     VVVVPASVQISDGETSASVDIVGAGRGQATITVSAAGFADVTADVEVYNVAYDSTSYY
                     GPGLFTNGANGGVGFDAWTISHNDGGHDGYTNFAGAFLGDSTASGGGDVNAVSNNAFG
                     FFANQVGDDTGTNEPIVEATRGFTALAMGQSLTLDLGVNFRNGAKGMMLQNGDTWLFE
                     VVVTADQYAWQNHALGGALQDLGWVYAADSAINVTVTRVGQDKYDIGLTRSGSAPEYM
                     LLEGVSFNHQAPDRARFYAWNTEQGGENNLYVNRLGINEFEALEMEGVINIDVGGTTT
                     IFIQRSDTTGPLTVDLSSAAPSVVTVPATVEFADGDAVTSFVANGVSESLTTIHAEAD
                     GYVGAPLLVNVFDFPGEFDNAGNYVPGLIVPTTFTNESNGGAGFGAWQFSALSDGAAI
                     QLTDSRVSTAWMNSFNDRSFSFIGGTKSNYASAYRDLNTALAEGDKFECLLGMNWSGG
                     NRGLDLQDASGNKLFNYDLSYENISAISFGLDTPVDLGWAYNATAVVQVVAEQLAGNQ
                     LSVTLTRNDGMTTNVVSAGLAAPVGRINFYNGGHDGGSPNFTLYANDLLITRGGGTTD
                     GIPNAWWDKFGVAASNRVASLDVDVDGSPNDDEYAGDTDPTDATRSFVNRVLAIRGNT
                     LMTLQVGPNTTNSRTYDVWWSTDLQADPQVWTPYNLNAPAAVGGGALDMLITNDTPRK
                     FFRTSVNIP"
     gene            81598..82236
                     /gene="plsY"
                     /locus_tag="PK634_00785"
     CDS             81598..82236
                     /gene="plsY"
                     /locus_tag="PK634_00785"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOW96301.1"
                     /translation="MNWLCHAAFALGAYLLGSVPFAYLFARLNGVDIRKVGSGNVGAT
                     NVFRAVGKGWGIATFAADALKGFVPAWLFPLLARRWGVECGPELGLLCGCAAIAGHSW
                     PVFLRFKGGKGVATSAGMLLGVAPAAVGIGLAVWVAVFVIARYVSVASIAAAFAVPAA
                     AWGLYGRAGALLPGVLTVLGALVIWRHRSNLARLRRGEEHRWGRPRSGAAHP"
     gene            82332..84041
                     /locus_tag="PK634_00790"
     CDS             82332..84041
                     /locus_tag="PK634_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOW96302.1"
                     /translation="MSSEAPTGRVARRFWVLSAVIAILGVGLSLGVWRYRALSETRAL
                     EAEFLQEAGTLRRATEREIRLFLDVLDSLRVLHTLSARISDEDFEEFVLKGMLHQQRV
                     LGGFGFVRSIPHDQRASLEQEPDASGRPALKLVESDGRGGYAPAAAREQYFALTYQTQ
                     PERLGLPAGYDFASWAPAARAIALMETRDGPVLGGVATGGPDGTPPGWYVFSPIYYVA
                     LDDVVFPPPGWLVGFVTALFKPSDILDRVLLGVPALRFEIELVERSAPESGRAALRYE
                     HPVRVADREWRFRCRPGPAYPRPRGLRESGLFLAIGLGLTALISWQLATVGRRTRQIE
                     RLVHVRTAALREAHERIAQEMNERMRLEDEIHSTAAREQQRVGQDLHDSLGQKLTGAL
                     FLSRGLLRQLEQAGRTEQASGRQLHDALKDTVAEVRRMARGLAPVSIGEDGLADALRR
                     LADESRQTFGVACVFRQSGGHPPAGAVAEQLYYIAREAVHNAARHGQPQRIILELAEE
                     DGRWRLVVEDDGRGLPPDAQKKGGLGLRIMRHRAKTVGAQLEIAAGAGGSGTRVICRP
                     SAG"
     gene            complement(84107..85786)
                     /gene="recN"
                     /locus_tag="PK634_00795"
     CDS             complement(84107..85786)
                     /gene="recN"
                     /locus_tag="PK634_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOW96303.1"
                     /translation="MLTTLRVKNLALVENVRVEFQPGLNVITGETGAGKSILIGALNL
                     LLGERADHDLIRAGEDACGAEACFRLADASAVDEALESFGLEPCADGQLIVRRIIRAS
                     GASQAAINDSPVTLPVLKKVGELLVDMHGPHDHQSLLSPEFQTDLLDAFGHCWDLRAA
                     YEEIHAQLQGLEERVRQIEQNGATAEQIDLLTYRITEIEQAAPVDGEEDKVIEEHRLQ
                     GSAQQVLELGGAALNALQDGEGAAFDALAAAQKALDGLSRLAPRAADWRQETGALAGR
                     LQELAADLRRFLEGIEADPARLAWLDERLATYQRLKRKYAPTVTEILQVLERDRGRLH
                     DLKTRGEQLAALGKEIEQARTKRDRAGAALRARRREHAGRLAEAVTRELRALGFSHGA
                     FSVEVREGQPRLSGMDDVDFGFAPNAGEPMRLLRAIASSGEISRVMLATKAVLAAHDR
                     IPVMVFDEIDANVGGEMGSAVGRKLAEVARRHQVLCITHLPQVAACGAAHLAVSKSVR
                     DGRTVTDVRRLDARERVDEVARMLGGRDLTSVTLKHARELLEKSGAAASPP"
     gene            complement(85806..86264)
                     /locus_tag="PK634_00800"
     CDS             complement(85806..86264)
                     /locus_tag="PK634_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOW96304.1"
                     /translation="MIAEGPWHINITIWSGFAAWMLAQLIKLLLYIRRTGRFDPAFML
                     RLGGMPSSHSATVTAVALSVGLRTGFSSTLFALALTFAVIVMIDAQTVRRAAGLQARL
                     LNQIVDELFREHHISQQKLVEFLGHTRLEVLFGMLLGVFTALLIHGKLGM"
     gene            complement(86332..87423)
                     /gene="gcvT"
                     /locus_tag="PK634_00805"
     CDS             complement(86332..87423)
                     /gene="gcvT"
                     /locus_tag="PK634_00805"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOW96305.1"
                     /translation="MKQTPLHDAHRQLGARMAPFGGWDMPIQYEGILQEHEDTRRRAT
                     VFDTCHMGEFALSGPGAQADLERLLTQSVATIPAGRCRYGFLLRDDGGVLDDLTCYRF
                     GPDRFMLVVNAGTLDRDREWIRSHLSSGTRFADTSEATAKLDVQGPLSRQAMEKAFQT
                     PMPDLPYFGFAETPLAGVPCLVSRTGYTGEWGYELYFPADRAREFWDRLLAVGVKPAG
                     LGARDTLRLEMGYPLYGHELTEDRTPAGASRGKYIDLTKDFIGGAAVAREIEGGTTAL
                     LAGLRLESRRAARAHDRVMKDGRAVGEVTSGSLAPSLGVAVALAYVAAPLAEVGTALA
                     VESRGNLLPAAVTALPFYTEGTARRKTGA"
     gene            87525..88253
                     /locus_tag="PK634_00810"
     CDS             87525..88253
                     /locus_tag="PK634_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOW96306.1"
                     /translation="MNSDLSPILRGWDYDPGRVNARWIIGRDGRLKVQLRLDLGVLQM
                     EPEGRPDGRRPRGHDSLLDYYRSLQADMPALDPAACAELQQEAMQYYYRYLAFHALRH
                     FDGVIRDTEHNLELLNLVEHHAADDDMAWAFLQFYPYIRMMNARARAEKNMREQRETD
                     AVSILQEAVSDIRSFWRRQGEEGAPPGSPEIEALTDLLGRIRRQGPRSRGEELREDLA
                     RAIAEEKYEDAARLRDQLRDIGEP"
     gene            88371..89219
                     /locus_tag="PK634_00815"
     CDS             88371..89219
                     /locus_tag="PK634_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOW96307.1"
                     /translation="MTIIDPHIHMYSRTTDDYTAMYRQGIRAAVEPSFWLGASRRYAG
                     TFFDYFQLILDFETARARRFGIDHYACVSVNPKEADNLPLAREVLDGMGPYLGHERCV
                     AIGEIGFNRITPGEEKVFQLQLELAKAHGLLVLIHTPHDTPEVSKRKGVERTLDILRE
                     FNYDDGRIILDHNTENTMDLARKRPVWAGLTVYPYSKLNPERVCGILRKWGLERTLVN
                     SSADWGVSDPVSLPKVAGFMKQQGFTPEEASRLLFENPMKFYSQHPRFKPQLDLPFID
                     PSTYQR"
     gene            89288..89866
                     /locus_tag="PK634_00820"
     CDS             89288..89866
                     /locus_tag="PK634_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96308.1"
                     /translation="MKLVLLGLGLLLLVALAADLRRMPKRKLPGFTDPADELQAVVLG
                     LNGPRYADPEGLFSIVAPAGWTAFKPPDSRPYDVVFRGPSGSTISVMATPVEYNDLPS
                     LVREIDRNEEEAGIRTQSEAFFFLGGPAVLRKAGLVTSRVFSVDFVRDRVAHHIICSV
                     PPELFDRYYPVLMDVVETYRPGAPKAAAVANE"
     gene            89859..90794
                     /locus_tag="PK634_00825"
     CDS             89859..90794
                     /locus_tag="PK634_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOW96309.1"
                     /translation="MSERLPAAGRARAWLQLLRVPNLLTVPGDPIAGFLLAAGRQAAS
                     GMAGRLSAAVAASLCFYAGGLLLNDVLDLETDRRERPFRPLPSGGASPRCALAAAVSL
                     LLAGVGLSAALGPRARDVGLALAAAVVLYNGGGKRAPFFGSALMGVCRALSLLLGATA
                     FPGLAGWPPLVVAGAGALGLYILSVTNLARREAEPGPVGRAKSWPVGALTLGLMVFGA
                     VTVRLGGGGAGLFVAPWFLAGSVAFLASVRLAGPPERARRIPAAVGALLRALLPLQAA
                     LAWGAGAGPAGGLAGIVLLAVWPVSRILGRRFYMS"
     gene            90803..92107
                     /locus_tag="PK634_00830"
     CDS             90803..92107
                     /locus_tag="PK634_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOW96310.1"
                     /translation="MNAPKKLLVVDVAALGWDIVSRAGAHARVFGFRPLEPVFPAVTC
                     SAQASFRTVAWPRDHGMVGNGLFFRELRRVMLWEQSAGLVAGARLWRRFRQAGRRVGM
                     MFWQQSLGEDVDLVLSPRPIHKHHGGMIQDCYSQPADLYARLIGEIGAPFNLMHYWGP
                     LASARSSDWIVEALARVMNSPDLAPDLLLGYLPHLDYDLQRHGPDSPAAARALERALG
                     YLARLRDEAEKTGYDFLFFGDYAIEPVTGPPVFPNRALREAGLLAARPVRGRLYPDFF
                     SSGAFALADHQVAHVFIGGGADDGRVEAVLRDLDGVAEVMDRGRQYELGLGPTRTGEF
                     ALLAEKGRWFAYPWWNEPREAPDYAGHVDIHNKPGYDPCELFFGWPPGRVSSDPARIR
                     GTHGRPGVPAAWAASFPVEPLPGTLLDLARAVEWRLDRSLDA"
     gene            92104..>92821
                     /locus_tag="PK634_00835"
     CDS             92104..>92821
                     /locus_tag="PK634_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOW96311.1"
                     /translation="MSARLQKDAVLRQSFAVPVEFPVIFTRGLFDPANPVLLKAVRRM
                     GERRRHRVAVFVDAGVWRAHPALARRIRAYFAAHTGALELVAPPQRVPGGEKAKRDAA
                     LVGRLLARFQAWRLCRHSFVVAVGGGAVLDAVGLAAALFHRGLRLIRVPTTVLAQNDV
                     GVGVKNAVNWRGVKNLVGTFSPPFAVLNDSDFLAALPDRDWIGGIAEAFKVALIRDAR
                     FFRWLSARASQLRARDRAAME"
BASE COUNT        13982 a        31269 c        32708 g        14862 t
ORIGIN      
        1 cggaccctcg atctgggcgg cgacaagttc gcctcgacgc tgaagatgcc caccgagctc
       61 aatcccttca tgggctggcg ggcgatccgc ttctgcctgg cccagcccga catattcaag
      121 acgcagctgc gcgccatcct gcgcgccagc gtccacgaga acgtcaagat catgtatccc
      181 atggtcagca acgtcggcga ggtcatgcgc gcgaacgcct tcctccagga ggccaagaac
      241 gagctgcgcc tgcgggacgt gccgttcaac gacgccgtcg aggtcggcgt gatgatcgag
      301 gtgccctccg ccgcgatcac cgcccacctc atcgcgccac acgtcgcctt cttcagcctc
      361 gggaccaacg atctcgtgca gtacacgctc gccgtcgacc gcgtcaacga gcgcgtggcg
      421 tacctctacg agccgaccca cccggcgatc atcgagctga tccggaacac gatcaacgtc
      481 gcgcaccagc acgggatctg ggtcggcatc tgcggcgaaa tggccggcaa cccggtcatg
      541 acgccgctcc tgctcggcct gggcgtggac gaattgagca tgagcccttc cgcgattccg
      601 ctggtgaagg aagtcattcg cagcctgcga tttgcggaag cagagggcct ggcccggaag
      661 gttcttccct ccgtcctcgc caccgaggtg ctcgaggcct gccgggacct cacccgtcgc
      721 gtggcgccgg atgtgcttga acttgtcggc tgaacagggc aaagtagccg ccccaatcca
      781 atgcggaagg agtcaaccat gtcacattca tcgtatctgt tcacgtccga gtcggtgacc
      841 gagggccatc ccgacaagct gtgcgacggc atttccgatt ccgtcctgga cgcctgcctg
      901 gcccaggacc cgaggagccg cgtcgcgtgc gagtcgctgg ccaagaccgg catggtcgtc
      961 gtcgccggcg agatcacgac gcaggccgtc gtcaattttt ccgacgtcgt gcgccagaag
     1021 gtcctcaaga ccggctacac cagctcggac atcggcttcg acggccacac gtgcgccgtc
     1081 atcgtcgcgc tcgaccggca gtcgccggac atcaagcagg gcgtggacgc caagaaggcc
     1141 gagggcaagt cgacggccga gcagggcgcc ggcgaccagg gcatgatgtt cgggtacgcc
     1201 tgcgacgaga cgaaggagct gatgccgatg ccgatcatgc tcgcgcataa gctcacccgc
     1261 ggcctggccc gcgtgcggcg ctcgggcgcg ctgcccttcg tccgtcccga cggcaagtcg
     1321 caggtcaccg tggcctacga ggacaaccgc ccggtgcgcg tggacacggt ggtgatctcg
     1381 acgcagcact cgccggacgt ctcgcacaag aagttgagcg aggcgatcat cgacgagctg
     1441 atccggaagg agatcccggc gaagatgatg gaccggaaga cgcgcttcct ggtcaacccg
     1501 acggggcggt tcgtggtcgg cgggccgcag ggcgactgcg gcgtcacggg gcgcaagatc
     1561 atcgtggaca cctacggcgg catggggcgg cacggcggcg gggcgttttc gggcaaggac
     1621 ccgagcaagg tggaccgcag cgcggcgtac atggcgcgct acgtagccaa gaacatcgtc
     1681 gcggccaagc tggcacggcg gtgcgaggtg cagttggcgt atgccatcgg ctatcccgag
     1741 cccgtgtccg tcctggtcga taccttcggc acggggatcg cgccgaacgc caagatcgag
     1801 aaggcggtgc gccgcgtgtt cgggctcaag cccgcggaga tcgtccgggg gctgaacctg
     1861 ttgcggccga tctacgaggc cacggcggcc tacggccact tcggccggac gaccgacttg
     1921 gacaatttca cctgggaaaa gacggacaag gtggacgcgt tgctgtcggc gctttgatcc
     1981 gccgcgcgcg cgggcgcgga cggggcggcc ggcgatggta ggtcttgcta tttggcgcgc
     2041 cggccgttac atatgaaatc ttacgcatca gacgggattt gacgcggaag aaaggagtgt
     2101 ttccatgaag gggcaactgg cctggtgggt gtgtctggga ctcgccgtga tcctggcggg
     2161 ctgcgcttcg ccggggaaga acgtcggcaa gctgcgcctc ggcgacacgc cggacgtggt
     2221 gctggacaag atgggcaagc cgaacaccgt ccgcgccgcc aaggtgtacg aggacgagag
     2281 ctcgctcgaa gtctgggaat acctgcccgg cgtcttcacg ctttatccga aagcctactg
     2341 gctgttcgtc gaggacggcc ggctggtcca gtggggcgag cccggcgatt tcgcggggaa
     2401 ggccggcggc ccgatcccgg tgggcgagta cagcgacaag aagcggctgg actgaccgcc
     2461 gcggccgtcc ccgggagacc tcggggcggg gcgccgtata ggttcgtgcg ccggccagtg
     2521 caacggagaa cacggggttg acccaccgca tccagcagag cggaccgccc ggcgccgggc
     2581 ggatcggccg cttgtggcga accgccctcc tggggggggc ggtttgcggc ctggcgggtc
     2641 ccgcctccgc gcagttcctg cgcctcggca tcttcgacgt caccgccgaa acaggcatcg
     2701 acctgatcta tacgagcaac gtcgagggcc agcgtccgtc ggaagccgag tacgacatga
     2761 cggactacta catggtcccc cggctccagc tgctgggcga ggcgcccttc ggccgcggca
     2821 cgcagctgac cctggacacc aacgtcggct tcgagaaaca cctgatccgc acggacctgg
     2881 acaacgagaa cgcgcccttc ggcaagctgc gcctcgacac ccgcaccgaa ctggcccggt
     2941 accaggtgga tctctacgcc gggatcgggc gtgaatacaa ggacgcggag gacatctacg
     3001 tccccggcga ccggaaaacc cgggatgtca ccgacaccca cgagtacggc ggcgaggtgc
     3061 tctggacctg gcgcgccctg acgctgggcg gccgctacga gttcaccggc gagcgccatc
     3121 tcgacccggc cttcgaggac ggcgaacagg atgaaaccat cgtcggctgg aacgccgcct
     3181 gccagctgcg ggaagacctg gccttgaact acgattacca gcgcacccgc accgtgctga
     3241 tcaacgaccc cgaggatccg acctgggaga tcgaggagag catcacggtg gactggcaac
     3301 tccccctgtg ggagcgcccc acgatcacct acagcttcgg cttcgagcgg gaaggcgtgg
     3361 tcgaggaccc ccaggaccgc tccggcgact gggacatcat ccacaccctc acggccgacg
     3421 gcgtgtggga cctgtcgccc accctgatgt tctccctcca tgcctcgtac gagtacgaga
     3481 gcgacccgga ggagaacgac gtgagcctga cctacggcct cgagctggcg cacgagctct
     3541 ccggctcggc gaggcagacc ctcacggcgg agcgcgagcc cgtcagcacg ttcggctcca
     3601 cggcggagac cgacaagacg accatcgaat acatcttcac caagagcgac ctgttcatct
     3661 acgacctgga cctcgacacg ggcgtgggct gggaactcaa cgtgcccctg gaagaggggg
     3721 aggacgtgga ggagatctgg gactaccacg tgggcctgac gcactcgcgc accctttcgc
     3781 ggcggcttgt gcgggacgtg agctacatgt atacttgtga aaagtcgagc ctggaagacg
     3841 agttgctgca ggagcaccgg gtgaccctga cgttcaccta cacgttctga tttcacgtcg
     3901 cacgggacgg gatgttgatg gccaatcatc gaatacgcaa cgggatgcgg cgcgcgctct
     3961 gcgtcgccgc aatgcttggg ctggcggcgg gggccttcgc ccaggagccg aagcccagcc
     4021 tcaaggggga gacggtgcgc cgcatcatgg cgggcgaccg gctccgcgtc tccgtgctgg
     4081 aggacccgtc gttgaaccag atctacaccg tcgccgggga cggcacggtg gacctgggct
     4141 tcgtggggcg gctgacgctg gaggacctgg cgatcgacga ggccgcggac atgatcgagc
     4201 gccggctgga ggagacctat ttcaagcagg ccaccgtgac ggtggaggtg tcggagttcg
     4261 tcgagggcgc catcctggtc gtgggcgcgg tggtgaatcc cggggccacg cccttccgcg
     4321 gggaccagat gatgacgctg atggaggtca tcaccatgtc cggcggcctg acccgcgagg
     4381 cggcgggcaa cgaggtccgg atcctccggt ggaagccggg cggcgggatg gcgcggcaga
     4441 ccctgacggt ggacgtgcag accatgctgg agacgctgga cttcagccgg gaccagttcc
     4501 tccggccgcg cgacatcgtc ttcgtgccct ccctcggcgc cagcgagaac cgcaaggagt
     4561 tcctggccct gggcgcggtg ggcgcgccgg gcttccaccc gtactcggag gggctcgacg
     4621 tcatccgggc ggtcatgcgc gtgggcggca tcgaccggga gggcgtctgg agcgccgcgc
     4681 gcatcctgcg gccggaccag tccggcacct accaggtcat ccccgtggac cttggccggc
     4741 tgttcggcgc ggccgagacc agcctgaacg ccccgctgct gccgggcgac atcttcttcg
     4801 tgcccttcgc cgagcaggcg agccgcgggc aggtgtacct gctgggcgag gtcgccaagc
     4861 cgggcatcgc ctccctgggc atggggcagg acacgaccct ggcccgcctg atcctgacct
     4921 cgggcggcct gggcaagttc gcgaacgaca gcaaggtgcg catcctgcgc tccgcgccgg
     4981 acgggacccg ccagacgctc tacgtggacg tgggccgcat catcaagacg ggcaatttcg
     5041 aggaggacgt gccgctgcag aacggggacg tcgtgatcgt gcccgagaaa ttgttgagct
     5101 tctgatgatg aaaagcatga tacagccccc gtcctccgcc ggcgccgcgg gcgcgcctcc
     5161 cgcctcgagg cccgaaaccc ctgcgcccct gccgtcccgg tccgtggaca tcttcgacat
     5221 caagcagtac ttccacgtca tcctcaagcg gatctggctg gtcgccctct gcttcttcgt
     5281 ctccgtcggc gtcacgatgg tcttcgcgct gcaggaggaa ccggtgtacc gggcgcgcgc
     5341 cacgctgctg ttgagccagg gccttcctct cccggccatc ctgcgccagg aggaaatgca
     5401 gctgcggggc gagtacctgg cgacgcagca gctgatcctc cagagcggca tgctcaagga
     5461 gcgcgccaag gagcggctgg cccgcgcggg cgagaagcag ggcgtctatg acgaactgcg
     5521 cgcgttccag gtctcggaga cctcgttcat cgccatcgag gccgaggggc ccgacccgtt
     5581 gtacgcctcg gcttttgtca acgccctggc cgaggagtac ctggatttca aggccgagga
     5641 gcggatggac acctcgcagg cgaccgtcat cagcctgacg cagcaggcga accggctgct
     5701 ggaggagctg aagcgcgccg aggcgcggat ggtcgcgttc gagaaggata acagcgtcgt
     5761 ggcgctccag gaccggggca acgtggccgc ctcgctcatg gccgagctct cgcgccgggc
     5821 gtccacctac cgcatggagc gcatgctgct ggagtcgcag cggccgctgc tgaccggcgc
     5881 ctcggacgac atcatcctga acaccctggc ctggccgtcc gcggccgccc tgcggccggc
     5941 gagcgcgccg cccgcgggcg cgggcgagga caaggccgcc gtcgcggagg gcgcggagcc
     6001 cgccgcggag cgcccggagc gcgtgctgga atggggcgtt gtcgagcggc ccgggtggag
     6061 cgacctgaag cgcgagcgcg cccggctgga ggcgcagctg gcgcagtacc gcgagaagtt
     6121 caaggacgag catcccgcca tccggcagac cctggacgac ctggccaaga accagcaggc
     6181 cctcgacgtc gaggtccagt tcgcgatgag ccagttcaac gcccacctgg agtcgctcac
     6241 cctgcaggag caggccaccc gccgcgtcga gaaggactgg gaggccgatg cgatcgactc
     6301 cacgcgcaag gcccaggaat accgcgccct ccagcggaac gccgagcggc tgcaggggct
     6361 gtacgatctg gtcttcaacc ggctcaagga gatcgacgtc tccatcggca tcgagccgga
     6421 gaccgagcgc atcatggaac gcgccaagcc cccgacctcg cccatcacca cgcgcaaggt
     6481 gcagtccatc gtcatcgcgg cgctggtcgg gctgggcatc gggctggccc tggtgttcgg
     6541 gctcgagacc atcgacgact cgctcaagta cccggaggag gtgaccaagt ggctcaacct
     6601 cccgttcctg ggcgtcgtgc cgacggccaa gtgggatccg caggacctgc ggacccacgt
     6661 gctggccaac atcgaccaga agagcgtcct ggcggagtcc taccgcaacc tccgctcggc
     6721 cttcctgttc agcggcgcca gcgaccgggt gcgcaccctg ctgatgacgt ccgccgtgcc
     6781 gcaggagggc aagaccacca cgtgcctgaa cctggccgtc agcctggcgc aggtcgggca
     6841 gcgcgtgctc atcgtggacg gcgacctccg ccgcggggaa ttgcacaagt tcttcggcct
     6901 cgagggcggg cgcgggctgt cggacgtgct ggtcggccag gccaagccgg atgccgtgat
     6961 ccagcggacc ggcctgccca acctggactt cgtggccacg gggcccctgc cgccgaacgc
     7021 ggccgagatc gtgctgcggc cggagatgaa ctccttcctc gactacgcgc ggcgcaccta
     7081 tgaccgcgtc gtgttcgact gcccgccggt gatggcggtc tcggaggccg tgatcctggc
     7141 ctcgctcgtg gacgccgtca tcttcgtggt ctgggccggc cagacctccc ggaagctgtc
     7201 ggggctggcc atccagctgt tgcgcgagcg cgggtcccac ctcctcggct gcgtgctgaa
     7261 caacctcgag ttcgggcgcg tgggctacta ctactattcc acgtactacg ggtactacga
     7321 ctacgagtcc gggtattccg ggtcggggac ccggcccggc gcggccgcgg cggcggcgaa
     7381 cggcggctac cggaagagcc gctgacccgg cgccggcgcg gcgggaaggc tgacgatgct
     7441 cgggaaatcg catattcgca tctcgctgcg gcgcatcgta ctcctcgcgc tcgacttcct
     7501 gtgcgtggcc ggcagcatcg tgctcgccgc cttcctgcgg ctcggccccg gggacggctg
     7561 ggactacgtg ctccaccacc ggctgaccct cggggcggcc tgcgcgatgt tcctgctggt
     7621 cttctacagc ggcggcatgt acgagcggga gcccctgctg aagcgcaagc gggaactggc
     7681 gcagctgccg ctgctgacga cggccatcgc cctgatcctg atcgtcgtgc tgttctacgc
     7741 ccgcgccggc gcgtccatcg gccggggcat cctcctgctg gcgggcggcc tgatctttct
     7801 cgggagctgg gggctgcgga cgctctaccg gatcggcatg ggctacggcc tgctgtcccg
     7861 caacgccctg atcatcggcg agggcaacga ggcgcgggag gtgctccgcc tgctggccgg
     7921 cgcgccggac gccggctacc gcgtgttcgg gatcatcttc ggcagccgga cgaaacacga
     7981 ggacctcgtc gagggcatcc ccgtgctcgg gcatatcagc cggctcaacg agttcgccga
     8041 cgcgtacgcc gtggacacca tcatcgtcgc gacgtcgctg gcgcgggagc acgccctgct
     8101 gcgcctgctc cgcccgctcc gctacgcggg ggtggccatc gtggactacg tggggcttca
     8161 cgaggagttg agccaggaga tcccgctgga ccacatcgac gacgaatggc tcatgtacgc
     8221 ggcgatgaac aactccgtga tccacatccg gaagatcaag cggggcatgg acatcgtcgt
     8281 cgccctgatc gggctggcgc tctccctgcc gatcagcctg ctggccgcgc tggcggtcaa
     8341 gctgacctcg cgcgggccga tgctctaccg ccagcgccgg gcggggcagg gcgggcagcc
     8401 gtacacgctg atgaaattcc gcacgatgcg ccaggacgcc gaggcgctca cggggccggt
     8461 ctgggcggac aagttcgacc agcgcgtgac gcccgtgggc cggttcctgc gcaagtcgcg
     8521 catcgacgag atcccgcagc tggtcaacgt gctgcggggc gagatgagcc tcgtcgggcc
     8581 gcgcccggag cgcccggagt tcgtggacac cctggcggcc gccatcccgt tctacaagga
     8641 gcggctgctc gtgccgcccg gcgtcaccgg ctgggcgcag gtcaagtatc cctacgccgc
     8701 cagcatcgag gcctcccggc ggaagctcca gtacgatctc tactacatca agcacatgag
     8761 cttcgtcctg gatatcctga tcctgctccg cacgttcaag accatcctgg ccgggctccg
     8821 ccacagcgag gacctcgagg cggcggccac cccggcggcg ccggcggacc cgacggaagg
     8881 tcaggccaag tgggcgtgag ccgggagcgc cccgcgacga tgaattccac ccgccagccg
     8941 cccccccccg aaatcggcga accggggccg ggggaggcgt gggtcgtgct gcatacccgc
     9001 ccgcgctgcg agaaaaagct cgaggccttc tgccggcggg ccgggatgcc ggcctacctg
     9061 ccgctccatc gcaaggtgca ccgctacggc gcgcgggagc gctcgttctg gtcgcccctc
     9121 ttccccggct atgtcttctg cgccgccacg gccgccggcc gctccacgct gcgccagaac
     9181 cagcacgtcg ccaacctgct ggaggtgctc gaccaggcgc ggctcgtcgg ccagttggag
     9241 cagatccggc aggccctggc cgtggacgac gtgatcgagg tcatgcccta tctcgccgcc
     9301 ggacgccggg tgcaggtgac ggcggggccg ttcaagggcc tggagggcgt ggtgcagcgg
     9361 gtcaagggcc gcaccaaggt cgtgctgaac gtggatatga tcgagcagtc ggtatgcgtg
     9421 gaagtggaca gcacctggct ggcgtcggca tgaaggaagc gacaaagtgg caaagagatg
     9481 ctgatttccc gaaccctgaa cccaccccca tgctttcctt cctgaaacaa ctggttcgcc
     9541 ggtacgcgcc agcggggctg aagcaccggc ttcgcaagtg gaacagctgg cggctggtcc
     9601 gccatttcga cgagggccgc tggccgccgg ccgcgatggc gaggcggctc gtgcggcccg
     9661 gggaccgcgt ggtggatgcc ggggcgaaca tcggctatgt gaccgcgctg ttttcgaagt
     9721 gggtcggccc ggccggccgg gtgtgcagct tcgagcccga gcccggcacg ttcgacgtcc
     9781 tggcctccaa cgtgcgccgc ctgaacctgg cgaatgtctc gctgttcccg tgcgcggtat
     9841 cctcgaggga gggggagggg gtgctggcga tcccggaata cgccgcgggc ggggagaacc
     9901 tgtacgaggc ccgggtggtg gacgccccgg cgcgcgcgcg cgggatccgg acggtcgcca
     9961 tccggctggg tgcgctggac aagctgttgg aggggctcga ctccctggcg ctgatcaagc
    10021 tggacgtgga gggctacgag ctggaggcgg tgcggggcgg ggccgggctg atcgggcgtt
    10081 tccacccggc gctgctcatc gaggtcagcg gcgacccgtg gacgccgggc acgaaggcgg
    10141 ccgccttgtt cgagctgctg aagggctggg gctacagccc gtaccgttcc gaggccggcg
    10201 tctggcgaaa gcttcaagcc ggcgacgccg tgctggacgt ggtgttcctc gccggtcctc
    10261 ctccgggtga atcgcctgtc catcattaac gcaactactt tacattaaga aggtagcgat
    10321 aatcatggaa ggcttcaaat cgctattgac gccgcgcctc gctttgagta cttttagaac
    10381 attctaaacg aacttccgcc atgttgcccg atcctgtcca atcggctcgc tgggaaatga
    10441 tcgaagcggg agggcgcacc gcccaatcct tcgggctggg ccggcttttc gggcagattt
    10501 tcatgctgct ctacttgagc cccgagccgg ccagcctcga cgaactcgcc agccagctcg
    10561 gcgttagcaa ggcgagcatc agcatcgcct gccggcagtt ggagacctgg ggcgccgtgc
    10621 atcgcgtgtg ggtgaagggg gaccgcaagg actactatgt cgcggagacg gcgtttgacc
    10681 gcatcctccg cagcgggctc ctgctttcgc tcaacaagaa actcgacacg gccaaggtgc
    10741 acattgagca gagcctggcc ctgctggaga aaagcggggc gaaggacaac ggggcggcgt
    10801 ttttgcgaac gcgcctccgg caggccgagc agtatcgcga gcggatcaac cggctcctgg
    10861 gcagccggct ggtccgggcc attttgcgga tttgagtccg gggcgccgga ggccggcgcc
    10921 gcatgaacag ggcaagacca gggcgggcgc aggccgttcc tcgcgggaac tgacgggggc
    10981 gaagcctgct tatctcccgc ttgcatcctt catccctcac ccttcatcct ttccatgtcc
    11041 ctctggtcct tccgattcct gctgtggtac atctgcatcc tcatcgtgca gccgcagcac
    11101 cgcttttcct tcctcatgcc gttccgcatc gcgaacctgt gcatggtgat cgccacgggc
    11161 ctgcacgtcg ccagcgccct gatcgagggc cgcccgatcc tccgcttcgg gccggccacc
    11221 aagctcgcgc tggccctcct aggcttcggg ctgctttccc agtacgcggg cgcgatgcag
    11281 acgtacacgg ggtggaacag cttcatcgac atgctggtga agaacgccta catgctgatc
    11341 ctgatcgagg ccatggcggt caccgtggag cgcgtctggg cggtgccgac gaccatggcc
    11401 ctggcctcgc tctggtggat caagggcggc ctgcgcctgt cgcaggcggg ggccacctgg
    11461 ggcatgggcg accgcatcat ggggcccgcc tccggcctcg tgcaggaccc gaatttcttc
    11521 gcctacatga tgggcgtgtt catgccgctg tacctctact actcgcggag cggagaccgg
    11581 aagctcgtcc gctgggcggg cctggcggtc gcgatcgcgt cgttcttcat ctcgctgcgc
    11641 acgggcagcc gagccggcct gctccagctc ttcgccctgg gcgcgctgct gctgccccgc
    11701 tactggttcc attaccggcg cgccctgctg atcgccgttg ccgcgttctt cttcctcatc
    11761 cccctgtccg gccggcagaa tattgctcgg ttcaagacgc ttcccgccag catcgcggcc
    11821 ttcttcaagg gcaaggccga gaagggggcg gggccggacg cctacagcgc gatggagcgg
    11881 cgcacgaaga tcgccgacac gtgggccctc atccaggagt acccggtgtt cggcgtcggc
    11941 atgaacccgg acgagtcgct gttccgggac aagtactggg gcgccaccgc ccaggtgcac
    12001 tgcgagatcc tgatggcggg ccgccagatg ggcttcatcg gcatggccat gcacgtgggc
    12061 ttcctgctga tccccttcct gatcgggcgc tcgatccaga agcggtgggc cggcgcctgg
    12121 ccggccctcg cggacctggg ctacaccgtc aaggtcatgg cgctcatgtt cgcgatcggc
    12181 ggctctttcc tgccgctgcc ctggcacccg atcctgctcg ccctgaacgg aaccgcctcc
    12241 gcgctgtacc tcaatctcaa ggccctggag gaggcggaac gcgcgggcgt cgcgccggcc
    12301 gggcgggtcc cggacacggc cggcccgctg cccacgaccg cgtagcccgg agccatccat
    12361 gagcgcgccc ctgctgaccg tctacgagtc gtccaacacc gaccgcctct ccatctcggc
    12421 gtggcgggag atgctcgccg agttctggtc gtaccgggag ctgacctggc ggctgatgtc
    12481 gcgcaatttc gccgcgcagt tccgccagtc gttcctcggc tacctgtggg tcatcctgcc
    12541 gcccgtcgcc acgacgatcg tgttcgccct gctgcggcag gcccagatcg tgaacgtgcc
    12601 gatggccgag ggcgcgatgc cgtacgtcct gttcgccctg atgggcacga cggtgtgggg
    12661 ctttttcacc caggtgacga tcaccaccac gcacagcgtc gcgggggcgg gcaacctggt
    12721 ctcgcgcatc tacttcccgc gcgaggtcct ggccgtgagc tccgcgctca acgcggtgat
    12781 caacctgtgc atccgcctcg tcgtgctggc cctgaccttc gcgctggcgc ggtacgtccc
    12841 gcactggcag gtggtcttcg ccccgctgct gctggtcccg ctgatggccc tggcgatggg
    12901 gctgggcctg ctgttcgcgc ccgtcaacac gatgatggac gacatgggcc ggatgctgga
    12961 tttcgcgttc cagttcggcc tgttccttgc cccgaccgtg tacccgacgc cgaggctggc
    13021 ggacatcacg gggaagtggg aactcgcgct gttctggatc cactcgctca accccgtctc
    13081 gcatttcctg cacgcgatgc agtcgctggt cgagaccggg acgttcgacg tcacccgcgg
    13141 cctcgtggtc tcgacgatcc tgtcgttcct ggccctggcc gccggctggc ggttcttcca
    13201 catctgcgag ccgttgctgg cggagcggtt gtgagcacgg agaaccggac gcatcgagcc
    13261 gaacaccatg tcctctgaac ccgccatacg cgtcgagcgt ctctcgaaga agttcgcccg
    13321 ctcgctcaag aaggcgatgg cctacggcct ggtggatatc gcccgcgtgg cgttcctgcc
    13381 gcggcgcgcc cgctcgccgc agttcgcggc gcgggtcgcc gacgcggcct ccgcgcccgc
    13441 gccggccgcg gacgtcgcgg agggcctgcg cccgtccgag ttctgggcgg tccgcgacgt
    13501 ctccttcgag gtgcggtccg gggagtgcct cgggctgatc ggggcgaacg gcgcgggcaa
    13561 gagcacgatc ttcaagatca tgagcggcat ctacggcccg acctccggcc gggtggagat
    13621 ccgcggccgg ttgagcgccc tcatcgaggt gggcgcgggg ttccacccga tgctctccgg
    13681 ccgggagaac atctacatca gcggcgccgt gctcggcatg acgacggccc agatcgagaa
    13741 gaagtacgag cagatcgtgg acttctccgg cgtgcgcgat ttcatcgaca tgccggtgaa
    13801 gttctacagc tccggcatgc acgtgcggct gggcttcgcg gtcctggcct acctggagcc
    13861 cgaggtcctg ctgatcgacg agatcctcgc cgtgggcgac atggagttcc agaagaagtg
    13921 catcgaccgc atcaacgaga tgcggcagag ccggatggcc atcgccctcg tctcgcacag
    13981 cctgtaccgg atcgagtccc tctgccaccg agcggtctgg ctggatcacg ggaaggtcgt
    14041 cctgtccggc ccggcgcgcg acgtggtccg ggcgtaccgt gaccggcaga tcctcctgtc
    14101 cgccgacgag gcgcgcgcga agcagggcgg gggggcggcg gccgtcgcgg aatcgccgtt
    14161 cttcaccgtg caccgggcgg atcccctcca cgaggacggg cgcgcggacg cgcggttcgc
    14221 gttcggggag acgctggcga tccgcctcgc cttcaccgcg aagcagcgga tcgagcggcc
    14281 gctgttcaac ctggccttcg tggcggccgg cgtccgggtg ctcgagacct cgatgctggt
    14341 ggacgggcag gccccggagt gcgtggaggg gccgggcgaa ctcgtcgtcc gcctgcccgc
    14401 gccgcgcctg ctgcccaacg cctacaccgt cgagctgttc atccggaacc gcgagggcat
    14461 ggtggacctg atcgacacgc agaccgtgac gcatttcacg atcacgtcgg acggcctgga
    14521 ggccatcccg caggccgggc cctacgcgct ggggcatctc atgcacggca acatcgtgca
    14581 ccagccctac gagtgggacc tgtcgcggat ccgtcaaccg gaagaggcgc gatgaacggg
    14641 attcttcgcg gatggcgcgg cgtggccgcg gcggccctcg cgctggcgct gctgccgggg
    14701 tgcgcctcgg gccccagcac gaccggcctg aaagtgctgg cccggaaagg ccggcccttc
    14761 gtgatcgtgc agaccgacag ggccaagggg tggggcgtgg ccaaccaccc gatcctgtgc
    14821 cgcgtgtccg accgccgcct ggccctctcc tactggatcg ccggcgacgc cgtcacggaa
    14881 ggcaccgcgc ccgtcgggtg gccggcctac agcgacgacg acggccgcac ctggcagttc
    14941 ggcgatccgt acgcgtggct cggcgagccg ccgcccccgg aagcccgcgt gatcgaggcc
    15001 gggagccggt tcgcccatta tgatttcggg tacttcatgg cggccgccgt cctgccctcg
    15061 ggggagcgcg tgcaccacga ccgcaccgcg gtcctcgcgc ccaccggctc gctcggctcg
    15121 atccggagcg cggacggcgt gcactggacc tcgcccgagc gggttccgct cgatgttccc
    15181 ctggaaggcg gcgccgcccc ggcgaacctg tacttcgagc agcaggccgt cgtgtccacc
    15241 ggcggcgtcc tctacgtggc cggctacggg caggttccct cggaggagtg ctacgccacg
    15301 ttcctgttcg agtcccgcga cggcgggcgg tcgtaccggt acgtgtccaa ggtggcctcc
    15361 atggcgcagg ccccgtgggg gatcgagggc gcggacgagc cggcgctggt cctgctgccg
    15421 gacggggagt tgctgtgcgt catgcgcacg ggcgccccgt ggagcggggg cggcatctcc
    15481 gtgtcccgcg acctgctgtc ggcgcgcagc cgggacggcg gccggagctg ggagtaccgg
    15541 agactgccga tcgccggcgt catgccgaag ctccggctcc tgaccaacgg catcctggcc
    15601 ctcgccacgg gccgaccggg caacagcctt ttcttcagcg ccgacggcgg ccggacctgg
    15661 ggacgggagg ttgtcctctc ccgcgccgac cagaagacca gcggctactg cgacctggtc
    15721 gaggtgtcgc ccgggcggct gctcgccatc tacgacagca tggacaccga cctgtccggg
    15781 atctggctct gggagcccaa ggaagccaac ggcattttcg gtgtctatat ccatgtgacc
    15841 cgcctgttct gacccgaacc ctccacccac gaggtcctca tgatcacgat gcgaccgctc
    15901 gatttcctgg aaaagaaccg ggtgctcatc accggcggca cgggctcgct cggcaagaag
    15961 ctgacgcgca tcatcctgga ccacacggcc acgacggtgg tcgtcatcta cagccgggac
    16021 gagtacaagc agttcgaaat ggagctgtcg ttcacgccgg cccagcggca gcgcctgcgg
    16081 ttcttcctgg gcgacgtgcg cgacggcgac cgcctgcgcc gcgccctcga cggcgtccgg
    16141 ttcatcatcc atgcctccgc cctcaagcag gtccccgcgg ccgaatacaa cccgttcgag
    16201 ttcgtgaaga cgaatattct gggcgcgcaa aacgtcatcg acgccgcgat cgacagcgac
    16261 gtcacccacg tggtggcctt gagcacggac aaggcggcca acccgatcaa cctgtacggc
    16321 gcgaccaagc tgtgctcgga caagctcttc gtctcgggca acagctacag cggccgacgc
    16381 ccgacgcggt tctcggtcgt gcgctacggc aacgtcgtcg gcagccgcgg tagcgtgatc
    16441 ccgttcttcc tggcgcggcg ccgggagggc gtgctgccca tcaccgaccc gcgcatgacg
    16501 cggttctgga tcacgctgga gcagggcgcc gccctcgtgc tcaacgcgct ccaggacatg
    16561 aagggcggcg agctctgggt gccgaagatc cccagtatgc ggatcaccga cctcgcccgc
    16621 gtcatcgggc cggagtgcca gaccccggtc gtcggcatcc gccccggcga gaagctgcac
    16681 gaggtcatga ttcccgtcga cgacggccgg cagacgatcg agttcaacac ccatttcatc
    16741 atccggcccg cctttccctg gtggggcgag aactggcaca tggagaaggg cgggcggccc
    16801 tgtccagacg gcttctacta cgggagcgac aacaacaccg actggctgga cgacgcccgg
    16861 ctcgcccgca tgatcgccga cctggacctg cccgaggccc aggcctgggc gaaggaaaga
    16921 gggatttgaa caggaggtaa cggagggagc ggaggcggga tatctccgcg gcctccgttt
    16981 cctcctgtaa aacaggctat gattccttac ggtaaacagt tccttgacgc ggaagacatc
    17041 caggccgtcg tcgacgtgct gaagtccgac tggctgacca ccggccccaa ggtcgccgag
    17101 ttcgagaagg cgctggcggc cttcaccggc acgaaggagg ccgtcgcggt cagcaacggc
    17161 acggccgccc tgcacgcggc ggtctacgcg gcggggatcg ggccgggcga cgaggtcatc
    17221 gtcaccccca tgtccttcgc cgccagcgcg aactgcgccg tgttccaggg cgcgacgccc
    17281 gtcttcgccg acgtgcgcga ggaggacctg ctgatcgatc ccgggaaggt ggaacgggcg
    17341 ataacgggga agacgaaagc gatcatcgcg gtggattatg ccggcctgcc ctgcgactac
    17401 gactcattga ccgcgctggc gcgtgcgcgc gggttggtgc tgatcgcgga cgcctgccac
    17461 gccgtcggcg gcgcgtacaa gggccggccg gtgggttcgc tggcggacct cagcacgttc
    17521 agcttccacc cggtcaagaa catgaccacg ggcgagggcg gggcgatcac gacggataac
    17581 gaggagtacg cgcgccgcat gaggatgttc cggagccacg gcgtcaccgt ggatttccgg
    17641 cagcgcatgg agtcccactc gtggttctac gagatggtcg acctgggctg gaactaccgc
    17701 atcaccgatt tccagtgtgc gctcgggatc acgcagctga agaagctgcc cggctggatc
    17761 acccggcgca acgagatcgc cgcgcgctac gacgccgcct ttctgccttt ggccccggtg
    17821 gttcgccccg ttcatccttc atccctcatc cttcatcctg cgtcggtcca cggccggcac
    17881 ctgtatgtca tccgcgtgcc cgcggcgcgg cgggcggaga ttttcggcgc gctgcggaag
    17941 caggggatcg gcgtcaacgt gcactatatc ccgatccacc tgcacccgtt ctaccggcgg
    18001 cggttcggca cggggccggg cctgtgcccg gcggccgagc gggcctacga ggaattgatc
    18061 agcctgccga tgtacccggc gatgaccgac ggcgaggtcg cggcggtgat cggcggcgtc
    18121 cgccatgccc tgggaggatg accatgagcc agaaaaagaa aaaaacgcgc cgcgcccgca
    18181 ccgagcagga ggccttctgg gccgggacgt tcggggacga ctacatcggg cgcaaccgca
    18241 gcgaccagct gctcgcgggc aacctcgccc tgttttcccg gatcctggcg cgcgcgcggg
    18301 acgtcgggag cgtcatcgaa ctgggcgcca acatcggcat gaacctcaag gccctgcggg
    18361 cgctgcggcc gggggccgcc tgcacgggcg tggagatcaa cgcgaaggcc gcgcgggaga
    18421 tggccgcgat cccgggcgtg aaggccgtgc gcgcgtcgct cttcgactac gaaccgaagc
    18481 cgcacgacct ggccttcacc aagggcgtgc tcatccatct caatcccgag atgctcccgc
    18541 tggcctacga ccggctggcc cgcgccggcc ggcggtacgt cgcgatcatc gagtactaca
    18601 accccgcccc cgtcgaggtc ccgtaccgcg ggcacagcgg gcgcctcttc aagcgcgact
    18661 tcgcgggcga gttcatggag cggcacccgg cgttccggct ggtggactac gggttcgtct
    18721 accggcgcga cccggtcttc ccgcaggacg acctgacgtg gttcctgatg gagcgacgcg
    18781 gaagccgatg aaggccgtgg cgctcatacc cgcccggggc ggcagcaagc gcatcccggg
    18841 caagaacatt aaaccgttct gcggtcggcc gatgatcgcc tattccatcg ccgcggcccg
    18901 ggacagccgg ttgttcgaac gcatcctggt ctccacggat gacgaggcca tcgagcgggt
    18961 tgcccgcgac gagggcgccg aggtcccgtt ccggcgccca gccgagctgg cgaatgacca
    19021 tgccgggacc gatgacgtcc tggtgcacgc gctggactgg ctggccgggc aagggggcgc
    19081 gccgcggtac gtctgctgcc tctacgccac ggcgccgttc gtgaccggcg cggacctgca
    19141 gcgcgggctg gatgccctgc aaaaggccgg cgcgatcacg gcggtctccg ttacgacttt
    19201 cgcgtacccg attctccgcg ccctgaaggt cagcgcgacg gggcggcttg agatgttctg
    19261 gccggagaac cggacgcggc gctcgcagga cctgcccgag gcgtggcacg acgcgggtca
    19321 gttctactgg ctggacgtcc cgcgctaccg ggccgagccg cgcctctttt cgagcgattc
    19381 cgtgcccgtg cccctgccgc gccaccgcgt gcaggatatc gacacgccgg aggattgggc
    19441 ccgcgcggag gccatgttcc aggccctatt gccctcccca tcctgatcat cctgtagtcc
    19501 ggtccggatc agacaggata acaggatgga cgggataatt tgagggaggg aagattgaag
    19561 ctgattttcc catcctgaaa atcctgtgat cctgtccaga ttagacagga taagttcggg
    19621 gcaggcatca tggaatacga agatcttaca gagaagatca tcggttgtgc ctacaaggtg
    19681 tacaacagga tgggtttcgg atttctggaa tctgtttacg agaaatgcat attgattgaa
    19741 ttgcggaaga cgggactcac cgccgagtcc caggttccca ttcgggtccg ctacgaagga
    19801 gaagttgtag gcgatttcat cgccgacatc ctggtgcagc agaccgtaat ccttgaattg
    19861 aaatcagtga ggcaacttgc cacggcccac gaagtgcaac tggttaatta cctggtggcc
    19921 accggcaaac cggtgggttt gctcctgaac ttcgctgaag gaggagtaga agtgaggcga
    19981 aaagtgcggg agctaacttc gccttcccaa tcctgagcat cctgtaatcc tgtccggatc
    20041 agacaggata acaggatgga cgggataatt tgagggaggg aagattgaag ctgattttcc
    20101 catcctgaaa atcctgtaat cccgtccaaa gcctttttcc ctgaaatgaa ttcttctccg
    20161 gtcttgctga ttcgtgccga tgcctcgccg cagatcggcg cggggcacgt tatgcggtgc
    20221 ctctcgctgg cgcaggccgc gagggccgcg gcttgggacg tggtgttcct gtcccacggc
    20281 gaccatgaac cggtacgccg gcgcgtggaa gccgccgggt gccgctggca atcgctggaa
    20341 cgcgtccaac cgcacccggg cgatctgaag gaaacattgg ccgccgcgcg cgaggcagcg
    20401 gcgtcctggg tcgtcgcgga cgggtacatt ttcgacgagg cgtaccaggc ggccctccgg
    20461 gacgccggct tccggctgat ggccgtcgac gatcaccggc acctcgagcg ataccacgcc
    20521 gacgtgctgt tcaaccagaa tatccacgcc gagcgactgc gctacgccgc cgatcccggg
    20581 acggtgttcc tgctcggccc ccggtacgcg atgttgaacc gggccttcct gccgtggcgg
    20641 gaccggcccc gcgagatacc ggcgatggcc cggcgggcac tggtttccct gggcggagcg
    20701 gatcccgaca acgtgacggc ccgggtcgtc gaggccctgc gcgggctgga tgtcgaggcc
    20761 cgcgtcatcg tcggggggtt gaaccggcat cgcgatcaac tccgggcgca gctcccggac
    20821 aacgcggaat tgctggtcga cgtgacggac atgccggcgc agatggcctg ggcggacctg
    20881 gcgatcctcg gcgcgggcag cacgtgctgg gaagcagcct tcctggggct tccggtcctg
    20941 atgatcgtgc tggccgacaa ccagcgccgc atcgcggcgg gcctggacga ggagggcatt
    21001 ggcgtgagcc tgggcgaaca cgcggatctg aaaagcagga tgatcgccga gcggatgggg
    21061 gagatgatgc ccgatgccgg ccgccggcgc gccatgagcg ccgcgggccg ggccctggtg
    21121 gacgggggcg gcgccgcccg ggttctggcg gccctgcagt ccgcgctggt ccgcctccgc
    21181 ccggccgtcg agtccgatgc ccgcgcgcta tgggagtggg ccaacgaccc ggcggtgcgg
    21241 tccgccgcgt ttcacccgga gccgatcccg tgggaggatc acgtgcgctg gttcgaggcg
    21301 aagcggcgct ccggccggac ggccatctac atggcggcgg acgaagaagg acaggcgctg
    21361 gggcagatcc ggtttgacgg aacggacggc agcgaggccg aggtggatgt cagcgtggcc
    21421 tcctcgcgcc gcggcgaagg ctgggggccg gcgctgatca gggccggctg cgaccggttg
    21481 ttcgccgacc gtacggcggc cgcggtccgg gccctggtca agccggagaa cgagccttcg
    21541 cttcgggcct tcgccggggc ggacttcgat ccggaagact ccgtcgcggt ggccggccgg
    21601 cccgtccgct gcttcatccg gaggaggccg catgcctgac aagacctacg tggtggcggg
    21661 ttgcaagtcg tggaaccggc tggtgttcga cgaaacgatc cgccgcgggc ccggcgcttg
    21721 gcgctacgtc ggcgatccgg cggaactgac gctggagcgg ctcgcgccgc tggcgccgcg
    21781 gtacatcttt ttcctgcact ggtcgtggaa ggtcccggag gagatcgtgt cgcgctacga
    21841 gtgcgtctgc ttccacatga ccgacgtccc gtacgggcgg ggcggcagcc cgctccagaa
    21901 cctgattgtg cgggggcatt cctcgaccaa gctcgcggcg ctgcggatgg agggttcctt
    21961 cgattccggc ccggtgtacc tcaagaagga tctgtcgctc gaaggctccg tggcggagat
    22021 actggtccgc gcgacgcatc tttccgccgg catgatccgg gaaatcgtcg ccacggaacc
    22081 cgcgccgcgg ccgcaggagg ggacgcccgt cgtcttcaag cgccgcaagc ccgccgagag
    22141 ccggattccc cgcgtcgact cgctggggca actgtacgat ttcgtgcgca tgctcgacgg
    22201 agagggctat cctcccgcgt tcctcgagca cgagggcttc cgctacgagt tccgccgggc
    22261 cgcgctccgg ggcgccggcc tggaagccga ggtccgcgtt acaccgatcc aggaggacaa
    22321 gccatgaacg cactggtgat cgccgcccat ccggatgacg aggtgctggg gtgcgggggg
    22381 accatagccc gcctcgcggg ggcggggcac gacgtgtaca tcctgatcct gggcgagggc
    22441 atcacctcgc gctatgactg ccgcgaggcc gccgacccgg ccgacttgga ggccttgcgc
    22501 gagaaatgcc gcgcgtcggc ccgccgcctc ggggcccggg acgtgttcct ggaaagtctg
    22561 cccgacaacc gttttgacac ggtcccgctc ctggacgtca tcaagaagat cgaaaagcgc
    22621 atcgagcagc tcaagccaga ggtcgtgtac acgcagcacg gcggcgacct gaacatcgac
    22681 catgtcacca cgttccgcgc cacgctgacg gccacgcgcc cgatgccggg cggctgcgtc
    22741 cgggccgtgt acgcgtacga ggtcgcctct tccacggaat gggccttcca gcagttcgag
    22801 ccggtctttc atccccaggt tttcgtggac atctcgggca cgctggagag gaagatcgag
    22861 gccatgaacc tctacgagag cgaggcccgg ccctatcccc atccgcgctc cgccgagatc
    22921 ctgcgcgcca tcgcgcaacg ctggggcagc gtgtccggcc tgccggcggc cgaggcgttc
    22981 gagtgcgtgt ggtccatcga gcaggacgcc tccgcccagg ctagcgcttg tacgggttgc
    23041 gcggccgctg cggcgcagaa cgggccgccc gcgccccacg cggcgggggc cggtctgaaa
    23101 gtggatgtcg tctgcgcgaa ctgcgaacac cccgtttggg tctggcttgg ggactggcgg
    23161 gcccggcagg gggcgggttg ttccgtgcgg ctgctgaatt gcgccgcgta cgcggacggg
    23221 ggcgatttcc tttttctcat ttcctgcggc gaaatcgtgc gaacgccaat cctgtcgaag
    23281 taccggcacg cgctggtgat ccacgccagc gacctgccgg agggccgagg ctggtccccc
    23341 cacatctggc agatcctggc cggccgcacc cggttcaccg tctcgctctt cgaagccgcc
    23401 gataagctgg acgccgggcc gatctggaag aaggaggtcg tggaactgga gggccatgag
    23461 ctgtacgacg agattaatcg gaagctgttc gacgtggaat tgcggctcat ggattacgcc
    23521 ctcgcccacg cggacacggt gaagccgcag ccccaggaag ggaccggcat gcagtactat
    23581 cgcaagcgta agcctgaaga cagccgcctc gaccccggga aaagcctggc ggatcagttc
    23641 gacctgctgc gcgtggccga tccccaccgg ttccccgtct tcttcgattt ccgggggcag
    23701 cgctacatcg tacaaatccg gaaggacaaa ccggagaccg gcgcatgagc gcggccttcg
    23761 ccatcgccgg ccgcgagatc ggcccgggcc ggcccgtcta cctggtggcc gagatgtcgg
    23821 ccaaccacgg gcaggatttc gacgtggccg tgcggatcct gcgcgccgcg cgggaggcgg
    23881 gcgccgacgc ggtcaagatt cagacgtaca cgccggacac catgacgctg gactgcgacc
    23941 ggcccgagtt ccggatcggg aagggcacgc tctgggaggg aaagaccctc taccggctgt
    24001 acggcgaggc gttcaccccg tgggagtggc agccgaagtt gaaggccgag gccgaatcgc
    24061 tcggcctgca gttcttctcg acgccgttcg acgacacggc cgtggatttt ctcgagggcc
    24121 tgggcgtgcc cgcgcacaag atcgcctcgt tcgagctggt ggacacgcgc ctgctgcgga
    24181 aggtggcgcg cacgggcaag ccggtgatcc tgtccaccgg catggcctcg cgcgaggaga
    24241 tcgacgaggc ggtcgcgacc ctgcgggccg ccggctgcgc gcaactggcc ctgctgaagt
    24301 gcaccagcgc ctatcccgcg cggccggagg aaatgaacct tcgcacgatc cccgacctgg
    24361 cgcggcggca cggtgtgccg gtcgggttgt ccgaccacac gctcggcatc agcgccgcgg
    24421 tcgccgccgt cgcgctcggc gcctgcgtga tcgagaagca cttcacgctc cggcgcgccg
    24481 acggcgggcc ggacagcgcg ttctccctgg aactgcacga gttcaaggcc atggcgcagg
    24541 ccgtgcgcga ggccgaaaag gccctgggcg cggtttcgta cgagatcgcc gagcacgaga
    24601 aggccagccg ggtgttccgc cgctcgctgt tcgtcgtcgc ggacatggcg gccggcgcgg
    24661 cgttcacgga gcggaacgtc cggtccatcc ggccgggaga cggattgccg ccgaagcatt
    24721 tggaacaagt cctgggccgg aaggccgccc gcgcgatcgc gcgcgggacg ccgctggcct
    24781 gggatctggt gagctgaacc cgatcggccg cacacaagga ggaaggggcc atgttgaggt
    24841 actgcacgaa atgcgtcatg ccggacacca agccggatct gcacttcgac gaggagggcg
    24901 tatgcaacgc ctgccgcaac tacgagcggc gcaagcatat tgactggaag aagcgcaggg
    24961 ccgaactgga cgaggtgctg gagcggttcc gctcgaagga cggggaacgc tgggattgca
    25021 tcgtgcccgt cagcggcggc aaggacagca ccttccaggt cctgcggttg ctggaactgg
    25081 cgatgaatcc cctgtgcgtg acggcgacca cgtgcgactt gtccgatctc ggccgccaga
    25141 acatcgagaa catcaagcgc ctgggcgtgg attacgtgga gttcagcccg aacaaggtgg
    25201 tccgccgccg gctcaaccgc atcgggctga cacaggtggg cgatatctcc tggccggagc
    25261 acgtgggcat cttcacgatc cccgtgcgca tggccgtccg gttccaggtg cccctgctgg
    25321 tgtgggggga gaacccgcaa cacgagtacg gcgggccgac gccggcggtg gagaacaggg
    25381 ccctggatcg ccggtggctg gaggagttcg ggggattgct cgggctgcgc gtgtccgacc
    25441 tggccggcat ggaggggatc gagcccaagc acctcatccc ctacacgtat ccccccgacg
    25501 aggcgctgaa ggccctgggg ctcacgggca tcttcctggg ctactatatg ccgtgggacg
    25561 ggtacaccaa cgccctgctg gcgcaggcca acggcttcac gaccttccac accaccgtgg
    25621 aaggctcgtg cgtgaactac gagaacctgg ataaccacca gaccggcgtc cacgactact
    25681 tcaagttcct gaagttcggg ttcagccgcg cgagcgacat cgccagtctc cacctccggc
    25741 gcggccggat cacgcgcgag gacgccatga gcatcgtccg gcggctcgac ggccggttcc
    25801 cgtggagcta cctcggcaag tcgctggcgg acatcctcaa cccgctggac atgggcgtgg
    25861 acgagttcat ccggatctgc gaccggttca ccaacaagaa actcttcaag accgatgccc
    25921 gggggcagct cgtgaaggac cgcttcggca acctggaaaa ggtcaactac gacaacgtgt
    25981 agaaaagaca tcctcccgtt ccctcgcgtc gtccggatcg agccggccag cctgtgcaat
    26041 ctggcctgcc gtcattgtcc taccggaacg gtgcgcatgc cgcgcgggct gatgaagggg
    26101 ccggtcttcg aacaggtgct gcaggccata cggaggaacc gggggcgcgt caaggtcgtc
    26161 gttttgtatc acggcggcga gcccctgttg aatccccggt tcttcgacat ggtcggccgg
    26221 ctcaaggcgg cggatccggc cttgcatgtg aagaccgtta ccaacggcgc gctcctgtcg
    26281 ccccgggcca tccgcaagct gctgcaatgc ggattggacg ctatcgagat ttccctggac
    26341 ggcgcgtctc ccgaagaaaa ccaatacatc cggcgaaaaa gcaacacgcg cgagatcgtc
    26401 tcccgcatca ggcaattggc ggagatggtc aagcggtcgg actcgaagaa gccggacgta
    26461 tatatcaaca cgacccagtt tattcgtggc cacaggccgc ccccggcccg tcccgcgcgg
    26521 ccgcccgcgt ggttgacccg gcggttggcc gggacggtaa cgggaataaa gaccgccttt
    26581 gcgattcaat ggccgggcac gtccgcgggc ggggaatacg aggtcatccc ggccgcgcgt
    26641 cgaaagaagg ccgcgcgccg ctgcgatcat gtcatgaaca ccgttaccgt ctgcgccgac
    26701 ggaagcgtgg cgccctgttg ctatgatctc acgcgccgca tgatactggg ccacgttctc
    26761 aagcagccgc tggaaaagat atggaataac cgggcctatc gggcgctgcg aaaagcgatc
    26821 cgaacaggcc gtccccacgc cctgtgcgca ggatgcgccg tcgtgggcgc cgccggctac
    26881 ttgcttcccc tgaaaggccc gaagactgtt gccgccgatg agccgcgtcc tggtcattga
    26941 ttacgggttc tgcaacctcg attccgtctg ccgggccgtg caggagtgcg gtggcgatcc
    27001 gctactcacg gacgaccccg ggcaggccgg ttcggcggaa cgcatgatcc tgcccggcgt
    27061 ggggaacttc gcggaggcca tgaaggtcat ccgccggcgc ggctgggacc gcgcgatccg
    27121 acgcgaggtc ctcgagaacg gcataccgct gctcgggatc tgcctcggca tgcagctgct
    27181 cgcggaccgg ggcaccgagg gcggtgacag cgaggggctc ggcctcgtgc cgggccgcgt
    27241 gatccggctg cccaaggtcc gcccggacct ccggatcccc cacgtcgggt ggaacgaggt
    27301 ggcccagacc cggtcctcgc cgttgttcga gggcgtcccc gaccggaagg atttctactt
    27361 cgtgcacgcg tatcatttcg aggtcgccga gccgtcggac gtgctcgggg tcacgacgca
    27421 cggcccggat ttcgcctcca tcgtcggacg cgggcgcgtg gtcggcacgc agttccaccc
    27481 cgagaagagc ctgcgatacg gcctggcgct gctgcgcaac ttcctgtcgg cgttttgagc
    27541 atgctcaagg tccggatcat cccgacgctg ctgtacaagg atcacggcct cgtcaagggc
    27601 atcggcttcg acagctggcg ccgggtgggc acggccctgc cggccatcaa ggtgtacgcc
    27661 gcccggcagg tggatgagct gatctttgtg gacatcgccg ccacccggga gaaccgggcc
    27721 gtggacttcg aggagatcga ccaactggcc gacgaatgct tcatgccgct gaccgtcggc
    27781 ggcggggtcc gtgccgtcga ggacgtccgg cggctgctgc gggccggcgc cgacaagatc
    27841 gccatcaata cggcggccgt ggagaacccg gaacttattc gcggcgccgc ggccctggcc
    27901 ggctcgcagt gcgtggtggt gtcgatcgac gtgaagcggc accccgacgg gcgcggcgag
    27961 gtgtggacgc atgccgggag ccggccggcg ggccgggagg cggtggcctg ggcgcgcgag
    28021 gtggaggcgc ggggcgccgg cgaaatcctg ctgacctcca tcgaccggga cggctccatg
    28081 ggcggttatg acctggacct gatccgggac gttgcggggg cggtgtccat ccccgtcatc
    28141 gcttccggcg gggcggggaa ctacgagcac atgcgggcgg cggtgcagga gggcggggcc
    28201 tccgccgtcg ctgccgcgag cattttccat ttcacgcagc aaacgccgct ggaggcaaag
    28261 gcctacctgc ggcagcaggg cattcccgta cgcctgtgaa cgaactgtcc gccacaaccc
    28321 tgacgttcat cgccgagcgc cgccaatggg ccgccgcccg cgagcagggc ttgccggagg
    28381 ccgcggatgt cgtggtgacc gccagcccgg agatctactt cgagtgccgg ctgcagggcc
    28441 gcccctgcct ttcgctgtgg gattacctgc ccgtggccga gtccgttcgg cacgagcagt
    28501 gggtgtggga ctggtgcggg cggatcggcc gggcgctccg ggagcgggag ggcgacgggg
    28561 gcttgtcggg cctgatcgcc tacgacgcct tccatgcgct ggtcgctgtg ctgaacctgg
    28621 cttgttccgc ctcccggctg ctggcggagc ggcggcccgc gcgggtcagg gtgttttccg
    28681 aaatgcagca gccactgctc tgggatgcct ccaccccgga tgcggacctc ttcaacttcg
    28741 tggtcgaccg gttggcgcgc ggcgggggcc gcgagatgct gttcctgtcg ctgacgccgg
    28801 cagtacgcga ggagattcgc ctggcggatg tgccgccgtg catcccggag ccggacgcgg
    28861 aggcggcgct cccgagcgac ccggttcgcg cgctggtgtt cacgcagagc atcagctggg
    28921 ccgagcagga gccgctcctc ccggtcctgc accgggagtg gggcggcggt ctcgtgaccg
    28981 tcatgccgga gccggccgat attcggggac cttacctttc ctggtcgcac ctgcgccggc
    29041 tgcccatccg gccgccgccg atgcccgatg agccggcgat gcaggcggcg ctggcggacg
    29101 cggtgtccga tcccgctctg ccggagtatg tgcgcatcct ggccggtccc gaatgcgccg
    29161 ccttcttcct gcgcggctgg cgggactggc tggccctggc atccgcggag cgcgggatcg
    29221 tccgcctctg tcaccgggcc taccgacccg gggtcgccgt gctgccctat gacatcgagg
    29281 gaaccgtccg gagcatggcg ttcgagtggg cgggcgccgg ggtgccgaca ctggccgtgg
    29341 atcacgtggg gctggccctc ccgtgcgtcg gcccccgcag cctgggcgcg ggcaacgcga
    29401 tcgccgtatg gggcgggccg gatgccgcac tgcatagccg atggcgcgat ccgtccgcgc
    29461 gggtgttcca gaccggcagc atgcgccgcg acctcgccgc cgccgcggag cggggaagga
    29521 tgcgggcggc ggcgccggcc tcgccggatc gcccggcggt tctgatcatc accgcgcagt
    29581 acgggtcgga actcatggtg aacgggggct acccggcgcc tggcgaggcg ctgtcgtggt
    29641 ggaccgaact ggcgcggctg tcggtccgca acctgaattg ggattggaaa ctcaagaagc
    29701 acccgcgctt cgaccacgag tctttctacc ggtatctcgc cgcgaccgtg gaaaaccggt
    29761 tcgagcccgc gacccccgac ctgctggagg ctctcgactc ggcggacgtg atgctcctga
    29821 tggccaacgc ctcgaccagc gcggcgcacg ccatcctcgc gggcgtgccg gtggtgtact
    29881 tcaagccgcc ggtgacgccc gagttctggc ggtcgcccct cgaggacggc ggcgccgtcg
    29941 tcgtttcgga tatgccgtcc ctggaggcgg ccctgcagcg gatgctggcg gacgcggcgt
    30001 atcggcgcga ggtggcggcg gcccagcggg ccgttctgga ccaagccctc gtggccacgg
    30061 gcgacgcgtc cgtgaacctc gtgcgcaacg ccatcgagga gcttcgccgg gacggccccg
    30121 cgccggccgc gcccgatccc gcggcccgtt ggatcgcgga catgaccatg acggtggacc
    30181 acggattgcg cggcgcctgg ccgtggcggg cgttccggcg gcggctgcgg caactcgccg
    30241 cgcgcgggcg gaagctggct ttcgacgacc tcgatttcct ggacgcggat cggttgcccg
    30301 attacttcat gaacatcgcc ctcctgtcca tctggcggcg gccggaagcg gaccgccgga
    30361 tcagccggcg gcgcccctgg ctgggccgca tcctgtggga gatccgccgc gccctgccgg
    30421 actcgatccg gccgccggcc ggctggctgc ggccgtacct gcgccggacg gtcgaggagg
    30481 aagccgccct gaacccggcg tcccggttct ggaacgccct gcggcggaga acggcggctg
    30541 catgaaaatc atgctcgtca acccgatcat ccggccggac cagccgcccg gcgatttccc
    30601 tgtcgggctc ggtatcatcg cggagatcat gcgccgggaa gggcacgatg tccgcgtgta
    30661 cgacgagaac gccctgcgcc ccgaccgcga gaccttccag cgcgacctcg cggccatgga
    30721 cgagcccgat gtcgtggggg ttggcgggct gatcaccatc tacgggcacc tcaagggcct
    30781 gctccccgcg ctgaaggatc gcttcccccg cgccacgctg gtgctcggcg gcggcgtgac
    30841 catcgagccg gacgtgatct tcgagaacat gcccgtggac tactgcgtgc acggcgaggg
    30901 cgagcacacg ttccgcgagc tgtgcgccgc gctggacgcc agcgccgcga aagacctgcc
    30961 gggcatcaag ggcatcagct tccgcgaccg cgtgaacggc ggcctggtcg tcaccccgcc
    31021 gcgcccgatc gagcggaact tggacctgtt cccgatgccg gcctacgacc tgttcccggc
    31081 ggaaacctac ttccggaaca accgcggcga ggtcgcgttc tggagcaaaa acgcggacgt
    31141 ccagcgctgc gccacgctgg tctggagccg cggctgcccc aacgagtgct cgttctgctg
    31201 gcgcatgatg ggccgcaccg tgcgcttccg ctcgatggac aaggtcatgg aggagatcgc
    31261 ccacctccgc tcccgctacg gggtggattc gtaccttttc gtcgacgagt gcatcaacgc
    31321 ggcgcgggtg aaatccgtgg agttcgccga gcgtctggtc cgggagggct atgccgcgcc
    31381 gtggtacagc cacgcgcggg cggatcggtt cgacccggaa ctcgcggccc tgttcaggag
    31441 gtcgggttgc gtcggcctca atttcggcat cgagagcggg aacgagatca tcctggcgac
    31501 gatgaagaag cgcgcgtcgc ccgagaaagc ggccgcggcg atcgcgatcg cccgcgaggc
    31561 cggcatcggc gcccagtgca ccttcatcat cgggacgccc ggcgaaagcc gcgccaccgt
    31621 gcgggattcc gtccgctgga tccgaaaaaa caaggtgcgc cacttctatt tcttctactt
    31681 cacgccgtac cccggctgcg aggagtacca ccggccggag acccaggagc gcatccgccg
    31741 gcgctacggc tcgaaggacg cctacttctc cgtgctgggc gacgcctaca agctggtggt
    31801 caacctgacc ggcgtctccg acgcccggct gacgaagtgg cgggaatggg ccttccgcgc
    31861 cgcccgcgag agcgcctggg agcgcgggaa gcgccgcgtc gcttcgctgg cctcgctcgc
    31921 cgggaaggcg ctgaaactgt tggtccagcc ttcgcgctgg cgcgcggcgc tgggtaaccg
    31981 gctgcgccgg ggcggctggt acgaattccg ctccccgtga acggagaggg aacaggagct
    32041 aacggaggga acggagaaga atccggattc tccgtttact ctgttacctc ctgtccatta
    32101 catgaagggg tatgcttgct tgagtaatac ggcgcccaag ctgttaatcc tgtgccatca
    32161 ccccgagccg aaggccgggc cgtgcatggt gacgctgcgc acggtattcc tgcgcggggc
    32221 gccgagctgc gcggcttccg ggcgccccga catcgcctat gcgcacctgg acgggctccg
    32281 gacgctgccc gggatcgttc gcgccctgcg cgccgacccg gacacgatcc tccttttcag
    32341 cagcgtcaac gccctgcgga agccgccggc gctctgggcg ctggcgctga acgtctggct
    32401 cctcaagcgg aaacaggtcg tgttctggca cgaggcgtcg tccgtggtcc gggtgaccgc
    32461 cggtctggag gggccctcgt ggtggcggcg gatgtttcgc cggctgcact tcgccgcggt
    32521 ccgccatttc ctgcataacg gcctgacgtg gcacctcgcg gtgagcatgc agaccaagca
    32581 actggtgctg tttctgctgg gcgtccagcc ggagcgggtg ttcgtctcgg gcaacgccat
    32641 cgacgtggag cgttctcccg tgcgcgaccg ggcgcctgcg cgggcggaac tgcgcgcctg
    32701 catggccggc gatccctcgt ggcagaaggg cctcgacatt tttatcgagc tggcgcggga
    32761 gttcccggcg tggcgggggc agccggtgcg ctacacctgg tacggcggga cgccggagtg
    32821 gctgcggcag atcgaggccg acgcgcgcgg cgagcagatc cggcgggcgg gcgtccgtct
    32881 ggccgggttc gtcagcccgc tgccggacgc gctgaaggac gaggacctgt tcgtgtttac
    32941 cagtcgatcc gacggcttcg ggctggcggc cctagaagcg ctggcctgcg atatcccggt
    33001 gttctgcttc gattccgccg gcatcgcgga aatcctgccg ccggaattcg tctgctcgtg
    33061 cccggccgac ctgaagaaga aaatcgaggc ctatttgaag cgccgcgacc gctacgcgcc
    33121 gggcgcgttc cggaacatcg cgctgggctg cggccgcgag cggttcctgg agacctggcg
    33181 gatgctggag cgcgcgatcg ggttgaccgg gggattgccc agcccgtgag gacgggcctt
    33241 gcgccccgtg agagaggaag gcatgaagat actggtggcc ggcggcgacg ggttcctcgg
    33301 ctcgcacatg gtggaccgcc tcgtcgagga cggccacgag gtcgccgtgc tcgaccggtt
    33361 tcccgacaac cggacgcgcc acctcgggca cctggagggc cgcgtccgcc tggtcccggg
    33421 cgaccgggcc gccagggaaa ccgccgcgga ggccttgaag ggccaggacg cggtgtacga
    33481 ttttgtttgc gcgacgaatc ccgcggtcag ctggaacgac atcccgctgg agatcgagca
    33541 gaacctgctg cccgcggtcc aattcttcga gatggcggcg gaagccggcg tccgcaagct
    33601 tgttttcgtc tcctcgggag gaacggtcta cgggcccgcg gagggggccg ccgacgaggc
    33661 gcgcgtcccg ctaccggtca acccgtacgg gatcgtcaag ctggcctgcg agcacttcct
    33721 gcggcatgtc cgggccaagc gcgggctggc ctatgatgtc taccgcgtgg ccaatgccta
    33781 cggcccgcgc cagtccgtca cctccgccca gggcgtggtc gcggtctgga tgcgccggat
    33841 cctcgacggc gaggttctgc aggtccgcgg cgacgagcgg accgtccggg attacgtgtt
    33901 cgtgaaggac atcgcccggt tgatgacgca ttccctgcgc gacctgtcgt cctccgacac
    33961 cttcaacctg ggcacgggga agggcacgtc gatcgtcgat ctgctggaga tattccggcg
    34021 cgtcgtggac gtgccgttcc gcttcgagcg caccccgcgc ggcccgagcg acgcgaccgt
    34081 cagcacgctc gacagccgga agctgctcca gcattatccc ggtttccgat tttcggacct
    34141 cgaggacaag gtccgcgaaa cctggcagga ttttcgcgcg cggcaccggt gcgcgtagga
    34201 cccaaacccg tgagagggag aaccccatga gcctgttcgt catcgcggag atcggcatca
    34261 accataacgg cgacctgaag atcgcccggc agctcatcga cgtggcaaag gactgcggct
    34321 gcgacgccgt gaagttccag aaacgcgcga tcgacctcgt gtacacgaag gaattcctcg
    34381 cggcgccccg cgagagcccg tggggcgcga cccagcgcgc gcagaaggaa ggcctggagt
    34441 tcggcgcggc gcagtaccgc gagatcgacg cgtactgcca ggagaagggc atcgagtggt
    34501 tcgcgtccgc ctgggacctg gagagccaga agttcctccg gccgttcaac ctgaagcaca
    34561 acaaggtggc ctccgccctg atcgtgtggg aggatttcct caaggaggtc gcgggcgagg
    34621 ggcggtacac gttcatctcc accgggatga gcgagctgcg gcacatcgac cgcgccgtcg
    34681 agatcttccg cgcggccggg tgcccgttcg agctgatgca ttgcgtgtcc acgtacccgg
    34741 tcgaggacga gaagaccaac ctgcgcggca ttcagaccct gcgcgatcgc tacgggtgca
    34801 aggtcgggta cagcgggcac gaggtgggcc tggcggtgtc ctacgccgcc gtcgccctgg
    34861 gcgcgacgtc gctggagagg cacatcacgc tggaccgcgc gatgtacggg tccgaccagg
    34921 cggcctcggt cgagccgtcc ggcctgcgga tgctcgtcgg cgccgtgcgg aagatcgaga
    34981 aggcgatcgg ggacggcgcg atcggcctca cgcccgccga gttgccgcag gcccggaagc
    35041 tgcgcgcgca cctgccggtg aggtgggaat gagcctggag acagcgaacc catgagcacg
    35101 atagccatca tccccgcccg gggcgggtcg aaagccattc cgaagaagaa catcatggac
    35161 ttctgcggcc acccgctgct ggcctggacc gtcgcggcgg cgcgcggctg cccggccatc
    35221 gacggcgtgt atgtctccac tgactgcccg gagatatccg ccgtcgcccg gcgctacggg
    35281 gccggggtca tccggcggcc cgacgacatc tcgggcgacc gcgcgacgtc cgagtcggcg
    35341 ctcgttcacg cctgccgcga actggagaag ggcgggaacc tgccggaccg cgtggtgttc
    35401 ctgcaggcga cgtcgcccgt gcgcgagagc gcggagctga acggcgccct cgagcatttc
    35461 gaccgggcgg gcctggattc cctcttcgcc gcctccgtgc ccgaggacct gctggtctgg
    35521 tgggaggagg ccggcgcgat gaaaagcctc aactacgatt tccggagccg caagcgccgc
    35581 caggacgccg agggcggcgg gcgcctgctg atcgagaccg gctcgttcta catcacgagg
    35641 acgccgattc tcctcgagac cggcaaccgg ctcggcggcc ggatcggcgc ctggatcgtg
    35701 ccggtctgga aaagctggga gatcgactcc gccgatggcc tggccctggt ggaggcgatg
    35761 atgaagatcc accggttgga ccggaatccc cccgtgccgg ttaacggata aatggctgtt
    35821 caaaaagaaa cacgaaagac aagcgagccc atcccatgac acaaaaagca caagaccgcg
    35881 tcgtgtactt caacggcgag ttcgtgcccg agtcggaggc gcggatctcc atctacgatt
    35941 ccgccctgat gttcggggac atggtcttcg agatgacccg ctcgttcggc aagaagcaat
    36001 tcaagctgcg cgagcacctg gaacgcctga tggccggcgt gaagatcctg cacatcccgc
    36061 tgaagcagac gatcgaggac ctcgagcggg cctgcctgga aacgatcgag cgcaacgagc
    36121 ccgctttcgc ggcggacgac gagcaccgcc tcatgatcga tgtctcgcgc ggcctgctgg
    36181 gcatctacca gggcgtggtg ggcctgcaca agggcccgaa cgtcgtgatc gccgatttcc
    36241 cgctgcgctg gaccgtcgcc ggcatgggca agctgttcga ggccggcatc aacgcggcgg
    36301 tcaccagcca gcgcgcgatc cccgccttcc tgctcgatcc gaagatcaag aaccgcagcc
    36361 gcatccatta cctgatggcc aacatcgagg cgtccaagct cgagggcgaa aacaactggg
    36421 cgctgctgct ggatcccgac gggttcatcg ccgagggcac gggcgacaac ttcttcatcg
    36481 tgaagggcaa gacgctgatc acgcccgagg gccgcaacat cctgcgcggg atcagccgcg
    36541 cgtacgttgt ccaggagctc gctccgcagc tcggcctgca ggcggtggag aagaacatcg
    36601 agccgtacga cgtctggacc gccgacgagg ccttcatgac cggcacgccg ttttgcctga
    36661 tcccggtgac ctcgctcaac cgcgtggcca tcgggacggg gaaggtcggg ccggtcgcga
    36721 ggtcgctgct ggaggcgtgg agccggaacg tcggggtgga tgtcgtgcgg cagatccgcg
    36781 actgggaccg gcagcggacg gccgacgcct cgggcgcgcc gacgccgtac tcgttcaagg
    36841 gcaaaaagta ggggatcggc atgaaatacc tcgattcgct cttctcgctc aagggccgcg
    36901 gcgcgctggt cacgggcgcg gcgcgcggca acgggctcgc catcgccgag gccctgctga
    36961 aggccggcgc cgcggtcgtg ctggccgacg tgctggcgcc ggagctgggg cgcgtcgtcg
    37021 cgcggctgaa gagggccggc ctcaaggcgc acgcgcgcgc ggcggacgtc acgaacccgg
    37081 acgagcgggc ggaactggtg cgcttcgccg agcgggccac cgggcacgtc gacatcctcg
    37141 tgaacaacgc gggcatcacg ctgccgcacg cggcgatgga ctacccggag gcccactggg
    37201 agcggaccta ccaggtgaac ctcaaggcgc cgttcgagct gtcgcggctg gcggcccgcg
    37261 ccatggcccg gcggcggcgc ggggtgatcc tgaacatcac cagcctcaac gccgaactgg
    37321 ccttcccgga caacccggcc tatatggcct gcaagggcgc gctcaagcag ctgaccaagt
    37381 ccatggccct ggatttcggc gcccgcggca tccgggtcaa cagcatcggc cccggctact
    37441 tccgcaccgc gatgacgaat gggagctggg gcgacccggc ccgccgggcg gcccgcgccg
    37501 cgcgcaccgc gctcgggcgc tggggccggc cggaggacct cgccggcgcc gtgatcttcc
    37561 tggcctcgga cgcggcctcg tacgtcaccg gccaggacct gtacgtggac ggcgggtggc
    37621 tgatcaaggg cctgtagacg agcgggggga attatgcgcg catcggaaaa agtgacggcg
    37681 aaggaatggg ccgcggtgaa gctgttggcc ctggatttcg acggcgtgat gacggataac
    37741 cgcgtcctgg tggaccagga cgggaaggag gccgtgctgt gccaccgcgg cgacggcatg
    37801 ggcgtcgggc tgctcaagaa ggagggcgtc ggcgtgatcg tcatctccat ggagccgaat
    37861 cccgtcgtgt ccgcccgctg ccgcaagctc aagatcgact gcatccagaa ctgcgacaag
    37921 aagctcaagg cgctgcagca ggtcgccgcg gcgcgcgggc tgaagccccg cgagatcgcc
    37981 ttcgtcggca acgacatcaa cgacctggag tgcctgcgct gggtcggcct gccggtcgcc
    38041 gtggccgacg ccgagcccga ggtactcgag gcggcggcgt acgtgacgaa gcggcccggc
    38101 gggttcggcg cggtccggga agtggcggaa cgaatccttc gggccgggta aggacggccg
    38161 cgccatggac gccgcgcccc gcctcatcca catgatgttc ttcccgtggg ggaaggacca
    38221 gaagctgcgg gcggaccagg agacgttcga ccgcaagccg ttcgagagca tggcgcggta
    38281 cgcgccggac ttcgaggtcc ggctctggac ctacgcgcgg gcgcgggagt tctgcgcggc
    38341 gaactacccg gcgatctggg agggaatcca ccggctcgcc cgcccgatca tgatgctgga
    38401 cgtgctgcgc tggctggtgg tctaccattt cggcggcgtc ttctggcagt actaccaggt
    38461 cccgctggcg gccatggacc ggatgctccc gtcgccgggc cggcaggcga aggtcttcac
    38521 cgagttcgtg ctctcgcccg aggaatgccg ccggctcgcg gcagagccca tccggaacgg
    38581 cgagccggaa gaatccatcc gcgtgctgaa ccaggttttt tccgccgtcc cgcggcaccc
    38641 gttcatcaag gccacgctcg acctgatcgt cgagcggacc tcgacgctga cgccgaagcg
    38701 ggactacgac atcctgttca tcagcgccaa cgccgccgtc agcacggcgt acgaccgttt
    38761 cggcaagaac gaccccacgg tcgaattggt ctcgcggccc gacacgcggg cgatgttcaa
    38821 gacgcaatac ctgggctcct ggcggacgga tgacgccgcg aagagacccg cgggcccaac
    38881 ggcggtttcg gccgtcgccc ggctggcccg ggccgccagg gcggcggtga agtcggtccc
    38941 gtgcctggcc ggcgcgtact accggtgggt ggagccgcac ccccacgaga cggcttttgc
    39001 ggaacggccg cccgcgcggc gggatgacgg gggcggcgaa tgggccggcg cgatcccggc
    39061 cctggccgcc gagttcggga ttcgttcggt gttggagtat ccctgcggcc cggcggcggc
    39121 ctcgcggcta gcggcctgga acgggctcac ctatatcggc ggcgacccgg ttcgtgaaac
    39181 cgtgaaacgc atcgggccgg ctggcgccgg cgcggtccgt ttcatgaacg ccatgtattc
    39241 gcggctgccg cgcgcggacc tggtcgtctg ccggaatttc ttcgtgcgcc tgccgtaccg
    39301 ggacgccctg gcggcgttga agcgccttcg tgcgagcggc gcgcgctacc tgctggcgac
    39361 gacgtttccg ctgctgaacg ccaactggga cggcgcgctc ggcgacgagc gcccgctgaa
    39421 catgaccctt ttgccctttg cgttccccga gcccctgcgc gtcctgccgg acccgtgccc
    39481 ggacggcagg acggaccgct cgctcgggct gtggacgctg gcggacacaa gacagtcgac
    39541 aattgagcac acatgagaaa agtagggcgc gttcgcggaa cgcgccacgg cggcttgggc
    39601 caagccgccc tacccgttga actcgatacc atgaaacgac ttgtattcgc cccggggctg
    39661 ggccagctcg ttaacgccgc cgccgcgctg acccaggatc gcgtccggga tcgcggttcg
    39721 acggaggagc acgaggacgt cctcgtcttc ttcgcgaacg gccggtacac cgacctcgcc
    39781 cgcgccatgc aggaggcggc gcgctgcctc tggagctggc gcgcgatcga gtgggcggag
    39841 gatgtcctgg tgtcgcattt cccgacgcgc cgcttcgcgc cgatcgcccg ggacgtgctg
    39901 cgcgagcgat tgggcgcgga cccggacgag atctgggtgt ccaagctggg gctggaggcc
    39961 accaagctga tcctgtacgc gttccccggg gcgcgcgtgg tcctgttcga ggatggcgcg
    40021 gaggagttca tcccgcagga ggtcctgtgc gggcgggcgc gcctgaagca gctgaagccg
    40081 gcgggctggc ccggcgggct ccgccgggag tggagccact gggggcgaac gtccgaatgc
    40141 ctcggcatgg aaggcctgtg cgtccgggac cgcgcgcggg tcgttcgcat gtattcctat
    40201 ctcgcgcggc aggtcgagct gccggcctac ctgtcgggcg tccccgtcgt gcacgtggaa
    40261 agcgagtcgc tgcggggccg attgcaggct ctcgcgccgc tgttcgaccc ggcgcagtgc
    40321 cggcagccgc cgaagcggcc gggggaatcc gcggtccttt tcctcccgca gccgttcgcg
    40381 gacatcttcc tgaccgcgga gaacgagtac gccctctacc gggcggccgt ctcggagatc
    40441 ctggacaagg gttattccat cctgtggaag gagcatcccc gcgagatcac gccgctggcg
    40501 ccgcggctga agcaggacct ggccggggac cggctgcaga tcctgcgcgt ccgccagcag
    40561 atccccgtgg aatgcctcgt cgccggctgg gacctggccg ccgtggtttc cgtgtcgtcg
    40621 acctcgctgc tgctcctcgg cggcttgtac ggctacccgg ccttcacggc ggcagatcgg
    40681 atatcgatcg attgctggcg gaagagaagc gattcggaac tggccaggct gtttctgcgc
    40741 accctgccgg gcctcgacca attgcccgcc gcccgggcgc aggagtagct tcgccgaaaa
    40801 cacagggata tgccatgact gctacactca aggaagccgt cgccgccgcg cccatgtacc
    40861 ggaccctttt cccggaaggc tggaaccggg aaaaacccct tcacgtgttc tgccgggccg
    40921 acgacgtgcg aacgatccgc cgcctgttca aggcgcggca cggcctgcgc gtatcgaggt
    40981 tcattttgcc ggacgatttc tggcagagcc ggtatcgcgc gaagatgctc ctgcgttccg
    41041 cgctgcgggg cctcggcatc cgcctgtgcc tgctcgatga cctggatccg gcctccgagc
    41101 cggcgctggt cgcctacgcc ccgcccggct acgaggtgtg gcagagccag cggctggccc
    41161 agggcgggtt ccgccgcgtc atgctctttg acgaccccgc ctacgacgag cgattccgcc
    41221 gcgaaacgga tttcctggag aagtactccg cgccgctgca cgcccttttc gagcggctgc
    41281 aggacgagga ctccgcgctg accctcgcct cggtggtgcg ccaccggcag acgggatcct
    41341 tcctgttcct ccggctggcc tcctatcccc agtacgggca tcccgtggtg cgggcggcgc
    41401 cgggcgacac gatcctcgat gtcggcgccg aggacggcgg gacctcgatc atgtactcca
    41461 accaggtcgg cgcgtctgga cgcgtgttcg ccttcgagcc cgatcccatg aacctcgaga
    41521 cgctgcgcgg gctgttcatc ggccgctcca acatcaccac cgtgccgatg gccctcggcg
    41581 agacggaggc ggaggtccgg ttcgtgagcc agaagggcgg cctgtctcac atcgcgcgcc
    41641 ccggcgaaac gggcgggatc cccgtgcggg tggcgcccgt ggacaagttc gtcgaggaaa
    41701 acaaactggc gcgcgtggac ctgctcaagc tggacaccga gggctacgag atgccgatcc
    41761 tgcagggcgc cgtcgagacc atccgccgct tcaagcccag gctgcagatc agcgtgtacc
    41821 acaagcagca gggccgcgac gacctggccg acgtgcccgc gctgatcagc cgcgttcagc
    41881 cctcctaccg ctactacctg ggccaccacg gctcctgggg caacgagacg atcttgtacg
    41941 cgatctgccc gtgacgcgcg cctcgttgag tttcatcctg cgccgggcgg agggcgtcgc
    42001 cttcgcggcg ctgctcgtcc tgcttgcgcc ggcgctgctg gtcgcggcca tcgtgcggct
    42061 ggcgcgcggc cgggaggcgg cgcggcggct gcccgcgatg tggcgggcgt cgttccggcg
    42121 atgggggaag ttcgtcgcgc acggcctgcg ccccgtgcgc gaggcgtggt ggccggcgat
    42181 gagctacgag ccgccgctga acgtctggcg ctatgttttc ctgctcgagc tgttcgacgt
    42241 ggtcaatgtg gcggcggcgg accgcccggc cgcggagccg ggggatcgcg cccgggtcct
    42301 cgtcgtcaag ctggcgcacg tcggcgacgc gctgcacatc gggccgtcgc tacgcgcgct
    42361 gcgggagcag cggccgggct gggcgctgga cctcgtggcc gggccgtggt gcgacgcgct
    42421 ggtccggaag atcccgtacg tcgcggagtc cgtgccgtac gcgccgcgcc tggtcctgtt
    42481 caaccgcggc cgaggggacc gcggcttgag cctggcgggg gaggcggctt tcttctggcg
    42541 tctccggcga aggcgttacg actggttgtt ctccacgtcg gtgaccagcc tggtggaggt
    42601 gtgcatgatt caggccgtgc gcccggcccg gtgggtcggc gcggcgcacg acgcgggcct
    42661 gtggcacccc ttcagcgagg cgcgcaccca gccctacacc agccggaaat acgaggcgca
    42721 acggatcgcg gacctgctgc cgctgatcgg tgtgcaggcc ccgcgcgcgg agttggaata
    42781 ctggctctcg gacggggagc gggccgaggc cgcccgccgg ctggccgagg ccgggctccg
    42841 cgccccggtg gttgccgtcg cgcccggcgc gggctggccc ggcaagctgt ggcccgtgga
    42901 gcgcttcgcg gaactggccg accgtctggc cgagcggcag aaggcatcca tcctgattct
    42961 cggcagcccc ggcgaacgcg agctagcgga gcaaatgaaa agccggatgc ggcagccggc
    43021 gctcaaccta gcggggcaga ctacgctgga ccagatggcg gcgctcctcg cggagtcgga
    43081 tctgctgatc gggaacgaca gcgcgccggc gcacttcgcc gtggccgcgg gcaccccggc
    43141 gttgtgcctg ttcgggccga ccagccggcc gcagtggggg ccgccggagg gcgggccgca
    43201 ccgggtgctg caagcgccga agctctgctt cgactgccac ccgtttcatc cccgcgcggc
    43261 ctgccacgac gaccggtcgt gcatgaagag cctttcggtg gacgcggcgg cggacgccgc
    43321 ggaagcgatg ctgcgggaca caagccatgt gcggaatcgc gggattcacg atgtttcggt
    43381 ccggtccggc tgacccggag gccgtcctgc gggacatggc gcggacgctg gagaagcgcg
    43441 ggcccgacgg cgaggggttt catcgcgacg gcgcgatcgc gctgggccac cggcggttga
    43501 gcatcatcga cctcgaaggc ggcgcccagc cgatgagcac ggccgacggc cggtacacgg
    43561 tgacgtacaa cggcgagatc tacaactacc tcgagctgcg cgccgacctg gagcgggaag
    43621 ggaccgcgtt ccggaccgcg tcggacacgg aggcgctgct ccagggttgg gcgaggaagg
    43681 ggcctgaggc gctgaaggat ttcagcggca tgttcgcctt cgcgctgtgg gatcgcgagg
    43741 cgcggacgct ccacctcgcg cgcgaccgga tgggcatcaa gccgctctac tggacccagg
    43801 tcgccggcga gctggtcttc gcgtcggagc tgaaggccct gctgcggcac cccggcgtgg
    43861 cgcggcggct ggacctgctg tccgtgagca agtacctcac gtacggctac atccccgcgc
    43921 cgcacacgat tttcgagggc gtccacaagc tcgagcccgg gacgcgcatg gagttcgacg
    43981 gggccgcgcc gcgcatggcg tcgtactggg atatcccgct ccgcgaccgg ccggtgagca
    44041 gccgctccgc ggacgagtgc gcggccgacc tgcgggtcct gctgcgggac gccgtgcggc
    44101 agcacttgcg cgcggacgtg ccggtgggcg tgttcttgag cggcgggctg gattcgagcc
    44161 tggtcacggc gctggccgcc gcggagtcgt cgcggccgat ccgcacgttt tccatcggat
    44221 tcgaggaggc cagctacgac gagtcgccct tcgcccggga ggtggcccgg gcgtgcggcg
    44281 tggagcaccg cgcggaggtg ctgtccctcc gccgcgcgct ggacctgctg ccgggggtcc
    44341 tcgaccggat ggacgagccg ctggcggacg cctccattct gccgacctcc ctgctggcgc
    44401 agttcgcggc gcgggaggtg aaggtggtgc tgggcggcga cgggggcgac gagctgttcg
    44461 ccgggtaccc ggcattccag gcgcaccggc tcgtcgagcg gctgtcgttt ctgccgatgg
    44521 cctggcgcga cgcgctgacg cgggccgcgc ggcacatccc ggtgtcgcac cggtacgcca
    44581 gcctcgagtt cctggcgcag cagttcttca agggcgcggg gatctcgccg gaggtccggt
    44641 tcctggtctg gctcgggtgc tgcggcaacg agcagaagaa cggtctcctc tcggcggacg
    44701 cgcgggaagc cctgcggcgg cgcgatccct tcgaggacgt gcatcgctac gtgcgccagt
    44761 ccgggctgat cggcggcttc gagcgcatcc tgtacctggc cgcgaagctg tacctgcagg
    44821 acgacatcct ggtgaaggtg gaccgcgcga gcatggccca ctcgctcgag gtgcgcgtgc
    44881 cgtacctgga cccggccgtg gtggagtacg cggcggggct gcccatggaa tacaagctcc
    44941 gtggcttcac gaccaagcac ctgctcaagc gcgcggcgcg cgggctgctg ccggaacgga
    45001 tcgtccagcg ccgcaaggct ggcttcatga tgcccgtcgc cgcgtggctc cagcgcgacc
    45061 tgcggccgcg ggtcgaggag ttgtgcgcgc cggaccggct cgcgcgggac gcgctctttg
    45121 atccgaaaac cgtgcgtcaa ctgctggacg agcatttcag cgaccggcgc gaccaccgga
    45181 aggtgatctg ggcgctgttc gcgttccagc actggcgcca gcgatacctg ccctgaatcg
    45241 agaaggggac gcctgccatg aagcggaaag tcacctgtgt cgtcggcacc cggccggagg
    45301 tcatcaagat ggcccccgtg atccttgccc tccgcggcga tccgtcgttc gacgcgacgg
    45361 ttctggcgac ggcccagcac cgggagatgc tcgatcaggc gctcgcgatg ttcggcctgc
    45421 gcgcggacgt ggaccttgac ctcatgcggc cggaccaggc ggtgggcgaa ctgaccggcc
    45481 gggtgctggc ggggctcggc gaacacctcg cgacgcatcg gccggatgcc gtgctggcgc
    45541 agggcgatac gacgaccgtc atggcgacgg cgatggcctg cttcttcgcg ggcatcccgt
    45601 tcgggcatgt cgaggccggg ctgcggagct tcgacctcca ggccccctgg ccggaggaat
    45661 acaaccggcg cgtggcggcc gtgggcgcgc gctggcactt cgcgccgacg ccgggcgccg
    45721 tgaaaaacct gcggcgcgag ggcgtggagg cggacaggat tttcgtcacg ggcaacacga
    45781 tcgtggacgc gctgcggcat atcctggcca cgacgccggc gccgcccccg cccgtgcccg
    45841 cgggccggcc gttcgtgctg atgacctgcc accgtcgcga atccttcggc gagcccatcc
    45901 gcgccatctt ccgggcggtc cgcgatttcg cggcccgcca tccggacgcg caggtctggt
    45961 atcccgtgca tccgaacccc ggcgtgcgcg gccccgcggc cgagatactg ggcggactga
    46021 agaacgtccg gctcgcggat cccgtggact acgtgacgct gctgcacgcc atgaaggcct
    46081 gccggctggt gctcaccgac tccggcggga tgcaggagga aggcgtgacc ctcggcaaac
    46141 gcgtcctcgt gctccgcgaa gtcacggagc ggcccgaggc cgtggagtcc ggactcgccc
    46201 ggttggtcgg aaacgacggc gcgcgcgtcg gggcggccct ggagcaggcc tggaccgatt
    46261 ccgcggagga agcgccgggc tcggccgtct tcggcgacgg ccatgccgcg gatcgggtcg
    46321 ccgccatctt gcgcgatcgg aaggagcgat catgacggaa aagaaacccc tgatatccat
    46381 tgtcctgccg acgtacaacg gcgcgaaata cctggccgag gccgtggaga gctgccgcgc
    46441 gcagacctgg cgggagtggg agttgatcat cgtggacgac tgctccacgg acgcgacgcc
    46501 cgacctcatc cggacgctgg cgcagaagga cccgcgcatc cgctccgtcc gccacgagcg
    46561 caaccgcaag ctgccggccg ccctcaacac cggcttcgcg caggcgcgcg gggagttcct
    46621 gacctggatc tccgacgaca atgcctatcg ccccgaagcg ctggcggaga tggccgccta
    46681 cctgctcgcg cattcggacg tcggcctcgt ctatacggac agcatggtcg tggacgagaa
    46741 cggccggccg ctgcgcgtgc ggcgcgtgcc cgagccggag acgttgcttg agcgcgaccc
    46801 ggtcggctgg agcttcctct atcggcgggc cgtccgcgag gccgtgggcg actacgcgga
    46861 ggagtttttc ctgtccgagg actacgacta ctggctgcgc gtctccatgc gcttcaagct
    46921 cgcgccgctg cacaaggacc tgttcctcta ccgccagcac ggctcgtcgc tgaccgcatc
    46981 gcgcgagaag gagatcgagc ggacctggcc gcgcatgctg gagaagcacc tgccgcatct
    47041 gggttgggcc acgccggggc agaaggcgga gtgctactac atgctcgccg cggccgccag
    47101 ggcccggcgc gatgtcgccg ccgcggcccg ctgctggctg ggcgccctgc ggaacgaccc
    47161 ggcctacgcg ctacgccggg ccggcggcaa ggtcgcgcgg ctgttccgga aacccgcccc
    47221 gccgcccgat tacaaggcgg cgatcgcgcg cgcccgcgaa tggatccggg ccaactccga
    47281 ggacggcggc gtctacttcg ccagctgccg gcgcgaggtc taccgcgagg ttaccggcta
    47341 ctacatcccg accctgctgc agatcggcga gccgcaggtc gcgcggcgct gggcggaggc
    47401 gatgcttcag gcgctggggc cggacggttc cgtgtcgagc atcgacggca agctctacgt
    47461 gttcgacacc gcccaggtga tgcgcgggtt cctgtgcttc gcggacgagg agccgttccg
    47521 cgaggggctc cggcgggccg gcgggtacat gctttcgcag ctccgcgagg acgggtcctt
    47581 ccccgacgtg tacgacggca agatcccgac gtacatcttc ctctacgcgc tcgagccgct
    47641 gctcgaggcc gggcggcggc tggaccggcc ggacttcgtc gagggcgcgc ggcgggcgct
    47701 ggattactac gagcggatct tcgacccgcg ccgctacgcg tcgaactcgc acttcttcgg
    47761 ttacgccgtc gaagcgctga tcgacctggg ccggccggag aaggcgcgcg aagccgcggc
    47821 atacgtgaag acgctgatcc ggcccgacgg cggcctgcct gccgcggccg gcgcgcgctg
    47881 ggtctgctcg ccgggccagg cgcagttcgc gcgcatcttc ttcaagctcg gcgacctgga
    47941 gaccggcgac cggctgctgg gctggatgct caagaaccag aacccgtccg gcggcttctt
    48001 cggcgcgtac gggaaggact cgctctactt ccgcgactcg gagccctgct gggcggtgaa
    48061 gttcttcgtc gacgccatcg tgctgcgcat ccgccggcac ttcgaggtgg agttcccgag
    48121 ctggatggac aacaagccgg tggccgagga tttccggttc aaggcgctgt gcggcgcgct
    48181 gccggaatcg ggttcgcgcc tgcgcgtcct cgagatcggg tgcggcgcgg gccggttcct
    48241 ccaggcgttg cgcgacctcc gcccgaacca cgagtacgtc gggctggacc tctccgaaac
    48301 cctggtccgg ctgctgcccg aggggatacg gggcgtgacc ggcgacatgc tgaacctgcc
    48361 gttcggcgcc gcggagttcg acgccgcgtt catggtcgag gcgctggaac acgcgcccga
    48421 cgtcgggcgg gcgatcgcag agctgatgcg cgtgctcaag ccgggcggcc ggtttgcgat
    48481 catcgacaag aacgccgcgt gcctcggccg gctccggatc atgccgtggg agcagtggtt
    48541 cgacgcggag ggtctgctcg gccgcctgcg cgccggctcg gacgacgcga aggtatccct
    48601 gatcccggcc ccggagcggt tcgcggacat gttcctgatc tggcagggaa cgaagaagcc
    48661 cggcgcctga catggccgcg cccgaccagc cgctggtcac cgcctgcgtg gggacctaca
    48721 accgcgagcg ctacctgcgc gagacgctgg acggcgtgtt cgcgcagacg cacccgcgga
    48781 tggaggtcat cgtcgtggac gacgcctcca ccgacggcag cgcgcggctg atcgaggcgg
    48841 agtacgggtc gcggctgcag ttcgtgcgtc tcccgaagaa ctcgggccgg ccggcggtgc
    48901 cccgtaacgt cgccgcgcgg ctggcgaagg gcgagtacgt ggccttcctc gattccgacg
    48961 acgggtggta cccggaacgg gtggcccggc aggtggcgtt tctcgaggcg cacccggaca
    49021 tcccgttctg tcacgcgtac tgccacctgc tggacgagga ctcgaaggtg ctcggcgtgc
    49081 ggcacgaggg cgcgctcccg ccgacgggcg actgcttccg cgagctggcg cggcactgct
    49141 tcatctcgat cagctccgtg ctggtgcggc gctcgctgtt ggacgaggtg ggcctgttca
    49201 acgaggaccc gcgctaccgg gcgcgcgagg actacgagtg gttcatgcgg atcgcgcggc
    49261 ggcgcccggt cgggctggtg gacgcggtgc tggcgcggta ccgccgggcc tcgtcgggga
    49321 tcagccaggt ggagggcgcg tggcgtacgc ggccgcagga cgccgaggcg caccgcctgc
    49381 tcctgaagag acgggatatc tgggagggcc gcgtgccgcg ccggcatatg aaggacgcac
    49441 tgctcaacgc cctcgaggaa aacacgatct ggtggcggga ccgcgggcgg gcagggcggg
    49501 cgctgtggtt cgccctgtgg gcctggcgga ccgcgccggc gcggccggcc gtgtggcgcg
    49561 aaggtctgaa aacactgggt cggctcatcg tgccgcgctc gcgaacatga catcggcgca
    49621 tcgaatcgag tacctggtcc tcggggccgg gccgaccggc ctgggcgccg cgctgcggct
    49681 ggaggaaaag gggctcggct ggcgcctgat cgaggccggg ccgcggttcg gtggcctggc
    49741 ctcgtcgtgg gtggacgacc gggggttcac gtgggacctc ggcggccacg tccagttctc
    49801 ccactacgag accttcgacc gctacatgga cctggccctc ggccccgacg gctggcaccg
    49861 ccacgagcgc gaaagctggg tatggctgaa gaaccggttc gtcccgtacc cgttccagaa
    49921 taacctgcac cggctcgacc ccgccgaccg cgaggactgc gtcgaagggt tgcggaaggc
    49981 cgccgcggcc cggaccctgc accctgaacc ctgccgcccg cgaaacttcg gggagtggat
    50041 cgatcagacc ttcggcgagg gcgtcgcgcg cctcttcatg cggccctaca atttcaaggt
    50101 ctgggcgacg ccgccggaaa agatggattt ccattggatc ggcgagcgcg tggccgtgcc
    50161 ggacctgaag gcggtgacgc gctcgatcga gacggggcag gaccaggtgt cgtgggggcc
    50221 caaccggacg ttccggttcc cgaagcgggg cggcacgggc gcggtctggg aggcgctggg
    50281 ccggcggctg cccgcggagc gcgtgacgct gggcgcggag atcgtccgga tcgacccgga
    50341 cctccggcag gcgaccgcgg cggacgggcg cacgtggctc tacgaccggc tgatttccac
    50401 ggcgccgctc aaccgcctgg tccgcatggc gcccgggatc gtggacgacc gggtcgccga
    50461 ccggctggtg tactccgcga cccacgtcgt cggcgtgggc atggacggga ccacgccgga
    50521 gcgcctgaag accaagtgct ggatatattt cccggagtcc aacagcccgt actaccgggt
    50581 gaccgtcttc acgaattaca gccccgagaa cgcgccccgg ccgggggccc agtggtcgct
    50641 gatgacggag acctcggaga gcgcggacaa gcccgtccgc cgcgagagcc tggcggagga
    50701 cacggtgcgc gccctggtcg aggacggcct gttgccggac cggcgccgcg tggtgtccgt
    50761 cgcgcaccgg cgcctgccgc aggcgtatcc gacgccgttc ctcgggcggg acgcgctggt
    50821 ggacccggcg ctgcgcgcct tcgaggcgcg gggcatcttc agccgcggcc ggttcggcgc
    50881 ctggaagtac gaggtcggca accaggacca cagtttcgcg cagggctacg aatgcgtcga
    50941 gcgcctcgcc gccgacggcg gggcggagta cgagccgacg ctgttcacgc cggacctggt
    51001 gaacaaccgg cgcaatccct gacggcggga tcaggagcgg cccaggcggc gccggatggc
    51061 gcgcggaact tgctggacaa tgcgccccag ccgggccggg ccgcggtccg gccagcgcag
    51121 cgtgtccggc aggttggccc ggcgcaggtc ctcaaggagc ggagtgcggt cgagccagtc
    51181 ctgctggacg aagaacgagt agtggtggac cagggcgtcg ggcgccagca cggcgaactt
    51241 gccgtgcgcg acgatccagt cgccccagcc gatctcgtcg aactcgggga tttcgccgag
    51301 ttcccgccag tggcggtagt cgtagcagag gcagttgatg ctgaaccgga aggcgaagcg
    51361 gccgaacggg ccgccctccg gcatcgagag gccgtccccg gcgatggccg gcgcccggac
    51421 ggcgcgcagc cgctcgttgg cccggcggag gtcgaggaac tcccgcgtga tccagtccgc
    51481 gatgcggccg ttgatccaga ccggcccgtc gaatttcgtc gtcagcggtt tgcggaacag
    51541 ccggtcgtac tcccgtgcca gttcgggaaa cgcgtggagc aggtagaagc agccgagcgc
    51601 gttgttcggg atgaccggcg tgatcagcga aaggccgggg tcgtcccggt gcgcctcgta
    51661 ggccgccgtc agcctctgcg gccagtcggc ggagacgaag gtgtcctcgt cgatcttgaa
    51721 gtagagcgag tccgggtggc gccgcacgca ctcgttctgg aggttggaga tcagccgccc
    51781 gcgcccgcgc ggcccggcga tggtgtccca cgggatgtca ggatgcgcgg cttgcaggtc
    51841 gcggacgtac cggaggtgac ggccggccac gccgttgagc agcaggacca cgcggtcgaa
    51901 gaagcggatc gaaccgcccc gcacgagcag gtccatgcac agccggaaac agtccagccg
    51961 gtggctggtc agcaccatga gtattttttt catcgtcagc gcttgcccgc gttgaccacg
    52021 gcgggcttcg gcagcgtgac ctcctccgcg ccgtccagaa ggcggtccgc aacctcgacg
    52081 ccctgcatga ccgaatgatc catgttggcc acttcgtact tccagccgcc gaaccggccg
    52141 cggctataga ggccctggct ttcgagcgcg ggcaggacat cggccaggat cgcgtcgcgg
    52201 tcgacggtcg gcaccgggta gccgaattcc tcgaccatgg tgaagaggtg ggtccgggcg
    52261 ggatcgcaat ccatgagccc tatgtcccgc aggccgcgca gcgtggccgc cccgctgtcg
    52321 ccgcggggga tcggcgcgtc gcccggcgtg gagatttcgc agagcaggga gcagtggcgg
    52381 tccgggtcgg gcacgtgcgc cggggagaag atcgagaagg gcgtgacgcg gtagaagtcg
    52441 caggccggct cggggcagta tatccacgtc ttgtcccgca gcgcgtcggg gatggggaag
    52501 gccggcgcaa cgcccgtgac ctcgacgtgc gtgtagcgga ggccgcgggc gcgccggacg
    52561 agtccggtgt cgccggccag ttcagcgagc cggatcagcg gcatggtgga gatcagggcg
    52621 tcgtagcgct cctcccggcc gtcctcgaag acggcgatcc ggcgtccggc atcgagccgg
    52681 acgaggccgc aggaaaggtg gagccggccc gcgggcagcc gcgcggccag cgcgcgccag
    52741 atggcgcccg tgccgccctc cctcggaaac tggaacgtgt ggttcgggcc ccaggagacg
    52801 tcgtcgcgtc cctcggcgat attgcgctcc acgcgcgcga ggtcgagggt gggcacgcgg
    52861 tcgccgatcc actggtagcc catgtcccgc agctcggtgg accagatctt gcggttgtag
    52921 ggcgccatga aatgcttcgc gatcccgcgc ccgaagccgg cgaggatcca ttcctcgaag
    52981 ttgttgggcg ttggacgttg ggcgtcggat ggtggacgtt gagcgagttc ccgcagcccg
    53041 gccaggcact cgtcgcgggc ggcgggcggc agatggcgga tgttgtactg gaacgggtag
    53101 gggatgaacg tctggtactc ccgcacccag gagcggcgct cgtggtggag gaagccgccg
    53161 ggcagcaggt cgtccatcag ccggtcgata tagtggtagt gggagtgcgc gacgtggacg
    53221 gcgaaatccc acgtgaagcc ccgctcgtcg cggaacgacg cggccaggcc gccgacgtag
    53281 gggcgccgct cgtagacggc gaagtcggaa tgcccttttt ccagcagccg gtgggccgcc
    53341 ccgaggccgc aggggccagc gccgatgatc acgattctca tgcgtgtatt gaaaagccct
    53401 tacactaaca ataggccgcg aaatcgcaac tttctaatac agcgaacagg agtgaacaga
    53461 ggtaacggag aatctttcag acctctcgtt ccctctgttg cctcctgtaa ttatccttcg
    53521 tgaaaatctc ctttttagtt cccgatattt actgcccggt cctgggcccg gtgaccgtgc
    53581 tggcccggac cctgcagcgg cattacccgg tccaggtcgc cggcccggac ttcgggcacg
    53641 gcgtctgccc gatgtaccgg ggcgcgttcg actacacggt cgtgccgacg ccccggctgt
    53701 accggcttcc ggattgcctg tgggagtccc ggaggctcgg tgccgcgctg acgggcgatg
    53761 tgatcgtcgc cgtgaaggcc ttcgcggaca cggtgcccgt cgcgctccgg gagaagcggc
    53821 ggcgcggggc gaaggtggtc gtctacctgg acgagtggga cggcgcgctg ttccaccaga
    53881 tgccgcgggc gtcgcgctgg aaatccgtgc tgcggaacct gcaccacccg ctggaggagc
    53941 cgtggaaccc gctggtggaa aagctgatcc cgaaggccga cctggtgatc tcgacgagca
    54001 cgtggctgca gaataaattc ggcggccgca tcgtccacat gggcgtggac acggatttct
    54061 tcaagccctc gtcgccggag cggacggcgg cgttgaagaa ggaatgcggg ctggaaggcc
    54121 tgcgctgcat cgtcttcggc ggcgtggtgc ggccgcacaa gggcatcgag atcatcctcg
    54181 acgccctggt ccggctcggc cgcgcggaca cgcggttcgt gatcgtgggc ccggtcaacg
    54241 agcacgtgga gaagctccgg tcggagccgt cgtaccggcc gtacctggcg acgctgggcc
    54301 cgcagccggc cgcgcgcatg ccggagttct tgagcctggc ggacctgatc gtgctgccgc
    54361 tcaaggacac gcccctggcc cgctcgcaga tgccgtgcaa gatcttcgag gccatggcca
    54421 tggcccggcc cgtgatcggc acggcgatct ccgacctgcc gctcgtcctg gagggctgcg
    54481 ggaaggtcgt gcccccggac gacgcggcgg cgctggcgga aaccatgcgg caggcgctcg
    54541 atcatccggc cgaggcggag gccatggggc gcgccgcgcg ggagaagtgc atccggctgt
    54601 acagccgcgg ggcgaccgaa cgcgaactgg tctcgatcat gaggggtttt gaacaggagg
    54661 caacagagag aacggagggg tgaggggagc ggcccgccct ccgttgcctc ctgttccatc
    54721 cctcctccct attggcttgc gcggtttttg ttaaacatgc acagtcggtc ctgcaacgcg
    54781 gagaacgtcc atgattaaag gcaaaaagat attcatcacc ggcggcgcgg gcttcatcgg
    54841 ctcaaccctg atcgggcggc tggtcgagca gaaccagatc gtcgcctacg acaacctggc
    54901 ccgcaacgcg ctgcagaccc aggcgtacaa gaaccatccc aacctgaagc ttgtcgtcgg
    54961 cgacgtgctc gactacgaca gcctcgcgaa ggccatggcg gacgccgacc tggtcgtgca
    55021 ctgcgccgcg atcgccggga tcgacaccgt ggtcaagagc acggtcaaga cgatgcgggt
    55081 gaacatggtc ggctcggcca acgtgctcga ggccgcctcg aagctgaccc actgcgaccg
    55141 cgtggtctgc ttctccacga gcgaggtgtt cgggaccaac gcgttccggt cgtcggagtt
    55201 ggacaagact tccatcggca aggtcggcga ggcgcgctgg acctacgccg tcagcaagct
    55261 ggccgaggag cacctggcca tcgcctacta ccaggaccag tggctccccg tcaccgtcgt
    55321 gcgcccgttc aacgtctacg ggcccggcca ggtcggcgag ggcgccatcc gcatcttcat
    55381 ccagcgcgcg ctcaagaacg agacgatcca gatcaacggc gacggcaccc agatccgcgc
    55441 gtggtgctac gtcgacgaca tggtccgcgg caccatgctc tgcatggagc acccgaaggc
    55501 cgtcggcgag tccttcaaca tcggcaacca gcgggcggtg acgacgatct acggcctggc
    55561 caacaccgtc atccgcgtcc tgaattcgaa gtcgaagatc gagttcgtct acaaggatta
    55621 cgccgacgtc gagctgcgca tcccgaacgt cagcaaggcg cgggagctgc tgggcttcga
    55681 ggcgaaggtg gacctcgagg agggcatcgc cctgacggcg gagtactacc gggcgcaagg
    55741 ctgacgtccc cgcggccgtc ttttcatcgt gaccgagcaa accacagtcg catcatcctc
    55801 cggctggatc gccgggtccg ccctggaccg gccgggcgag tgccggctcc ggttccatcc
    55861 ggcgggcgcg gacgagggca tccgcttcgt ccgctcggac ctgcccggcg cgccggaggt
    55921 cctgtgcggg ccggagcagt tgcgcagcat gccgcgctgg accttgctgg agcaggcggg
    55981 cgtcgcggtc catcacacgg agcacgtcct ggcggcgctg gcgttctgcg gcgtggacaa
    56041 cgcgaggatc gagctcgatt ccgagggcgt gccgatgacc gacagcggcc gttgcgcgac
    56101 gttccacgcg gcgctctccc ggctcggcac gcgggcgctg ggcgcgccgc gccgggtcta
    56161 tgccctggcg aaaccgctgt tcttcctcga ccggcagcgc acgacgggcg aggaatcggc
    56221 gcatcccgaa ctgcgcgacg ggcgctacgt cctcggcgtg ccggcggacc atttctcggt
    56281 gtcctacgtg ttccactggt cgcacctcga gggcttgccg atcggcgtgg ccgagtacga
    56341 cgaggccgcc cccgacgcgg cgcggcacgt gctggacgcc cgcagctacc tcgtcgagac
    56401 ggaaaccgag aaggtccgcg acctgctcgg cccggtgcgg gagaccgtgc tgatgctcta
    56461 tccgcgctgc ccgatggagc tgggcctcga ggcggcccgc cacaagatcg tggatttcgt
    56521 cggcgacctc cgcatcctcg gccggcccgt ccggggccgg ttcatcgcct tccggaccgg
    56581 ccaccgcatt caccacgagt tggtcggcca cctgcttcgt gataagatgc tgcaactggt
    56641 ctgacggcga acgcgtccgc tcgcggaacg gaagagaaca tgaacgctga accgctttct
    56701 cccggcctgg tgatcctggg cgcgggcttt cacgccaagg tcgtgatcga gacgatccgc
    56761 ctggccggcc tcggtccggt cctcgcggtc ctggacgatg ccccggccaa gcacgggacg
    56821 gcgatccacg gcgtgccggt ccgcggcgcc ctgggcctgc tgccggaatg gcacgcgcgc
    56881 ggcctcgtcc tgggcgtcgc gctgggcatg ggcaatatcc ggctgcgcga caaggcggcg
    56941 gcgctcatgc gctgggcggc gggcctcggc gtggacgcgc cgccgatcgt gcacccggcg
    57001 gccttcatcg cgccgtccgt cgcgcggccg cgcgggctct tcgccgggcc cggcgtcgtc
    57061 gtgaacaccg attcgcagct gggcgggaac ctcaccattt actccgggac cagcctcgac
    57121 cacgacaacg tgatcgggga cgacgtgttc atcagcccgg gcgtgcactc cgcgggcaag
    57181 gtccggatcg gcgcgggcgc gtacatcggg ccgggcgcga tcctgggctc ggccgtccgg
    57241 atcggcgcgg gcgcgatcgt cggcgcgggc gcggtggtcc tgaaggacgt gcccgaccgg
    57301 tgcctcgtgg tcggcgcccc cgcgcgcgtg gcccgcaccg tggacgaatg ggagaaggcg
    57361 cacccgtgat cattccgacc gccaagccct gcctcgacgc gtcggaccgc gcggcgctgc
    57421 ttcgcgtctt cgattccggc ctcgtcgcgg acggcgagga gatcgcggcg ttcgaggcgg
    57481 aactggcggc gcggctgggt ctccccggcg cggtggcggt ggggtctggt tttgccgcgc
    57541 tgcacctggc cctgctgggc ctgggggtgg gcgcgggcga cgaggtggtg gttccctgcg
    57601 tctccacatg cccggccatg cgcaacgccg tgtgggcggc gggcgcgacg cccgtctatg
    57661 ccgacgtcaa ccgggccgat ttcaacctgt cggccgagtc cgcgcggggc gtattgacgc
    57721 cgcgcgcgaa ggcgatcatc gcgccgcacc atacgggcgt gatcgcggac attcccgcgc
    57781 tggccgccct gggcgcgccg gtgatcgagg attgcgcgca ggcgctgggc gggacgtggc
    57841 agggccggcc cgccggatcg ttcggcgcgg cctccgtctt ttcgttctac gcgacgaaga
    57901 tcatcaccac ggtggacggc ggcgccgtgg cctcgccctc cgcgccggtg ctggagcgcg
    57961 tgcgcgggct ccggtactac cggcactgcg gcgacgaccg gatgcggttc aactacaaga
    58021 tgcagaacct cggcgcggcg ctgggccggt cgcagctgac gaagctgccc gggttcgttc
    58081 agcggcgcgc cgcgctgggc cggcggtacc tggccgcgtt ctgcgaagcc ggcggccgcg
    58141 aaagccagtc gctgcaccgg aacgatggat cggtctattt caaactggcc ttgcggctgc
    58201 cccccgcaac ccgcgaccgg ctgctcgggg ccgcggagcg gctcggcctg ccgtgctcga
    58261 ccgagttctg gtggatcgcg ccttcggccg gcgaacgcta cccgaacgcc gcgcgcctga
    58321 tgaagcgcat tgtcgcgctg ccggtctatc cggcggccac cgacgaggag gccgcgcgcg
    58381 tcgaaactgc ctttcgtcag gcgctgaagg aagccggccc gctccgggac ggagaagaag
    58441 catgagcgcg ccccgcaagc tggtcgcgat ccaccagccg aacttcttcc cctggctcgg
    58501 attcttcaac aagatcgccc gcgcggactg cttcatcctg ctggacaacg tccagctccc
    58561 gaagacgggc ggcagctggg tgaaccgttc gaaaatcctg gcggggccgg gcaaggacaa
    58621 gtggctgacg ctgcccgtgg accgctccta ccacgggacc cggctgatca ccgagacgca
    58681 gatttccatg ccggcgtccg agcgctaccg gctggcggac gtggtgcggt tcaactacca
    58741 aaaggcgcca gccttcgccg aggtattccc cgtgctcgag ccgctgctga agagcccgga
    58801 gatgaacctg gcggagtaca atatcctggc gatcacgacg ctggcacagc gcctgggcat
    58861 tcccggcggc cggttcgtcc gcagctccac gctgccgagc gcgggcgagg tctcgaccga
    58921 gcggctggtc acgctcgtcc gggaggtcgg cgggaacgcg tacctctacg gcgcggtggc
    58981 ggcggcgacg taccaggaaa acgagaagtt cgaggcggcc gggatcgcgc ctgtcgcgca
    59041 gaatttctcg cacccgcggt acgcgcagtt caacacgccg gaattcgcgg cggggatgtc
    59101 catcatcgat gcgcttatga acctgggctt tgcgggcacg cagggcctcc tgccggggaa
    59161 tacagcatga ccgagcccac cacccagacc acccgccccg cgctctccat cgtgatcctg
    59221 gcgttcaacg aggtggacaa cctccgcccg gtggtcgagg agacgaagcg ggcggtcgcg
    59281 gccaacccgt ggatcggtac gttcgagatc gtcgtcgtgg acgacggcag cacggacggc
    59341 accgccgggg tggcggacgc gctggctgcg gagaacgcga acgtgcgggc gatccatcac
    59401 ccgcgcaacc tcggcatcgg cgcggggacc aggacggggt acggcgccgc gcagggcgac
    59461 tatgtcatgg cggtcggggc cgacggcgag gtggagatgg cggaggtcct gcagcttgtg
    59521 aagctgatgg acggcgcgga cctggcggtc agccgccggc gccgcgggcc gggcgcgcac
    59581 cgcaagcttt tcacctacgg tttccaccgc ctgaccagcc tgatcgtggg cttcaatata
    59641 gagggcatgg aaggcattta cgttgtccgt cgggacatcc tgcgggacat ggagctgtac
    59701 tccaacacca gcctggtgaa tctggaagtg atcatgcagg ccgtccggag gggctgtcgg
    59761 atccggtccg gcgaaatcgg gatccatccg cgcttgagcg gcgagtcgaa gatgaccaac
    59821 tggcgggcga tcgcgcgggt gttccgggag atgctcaagt tgcgctggca cctgtggcgc
    59881 gcgggccggc ggaagaaggg gaagtgaaca ggaggaagca gggcgaacac agggcggaga
    59941 cggcctgttt cctctccctt gcctcttgtt cgtgaaagcg gagcgaatga agaaaagagt
    60001 ggaaatagcc ggcgcgcctt ccggtgctgc tttaacttat agctagcctc gtgaagaccc
    60061 tgaagcagaa ctggcggttg gggctgggcc tcctggtcag cgcgctgttc cttttcctgg
    60121 cgctgcgcaa cgtccactgg gcggacgtgc tgacggcctg gaagggggcg cgcccgtcgc
    60181 tgctggcggc cggcggcctg ctgttgctcg ggggctgggt cgtggcggcg gtccgctggc
    60241 gggtgctgct gtcgcccgcg ccgggcctgc gagtccgaga caccttcgcc tacatctgta
    60301 tcggcttcct ggcgaacacg gtgttgcccc tgcgcctcgg cgagatcgcc cgggccacgt
    60361 ggatcggccg gaagaagggg ctcggcgtca gccgcgccct gggcagcatc gcggtggagc
    60421 gggtgttcga cctgctgtcg gtaatcattg tcaccctgct gctgaccctg ctggtgcaga
    60481 tcccgccgat catccaggcc gggctggctt ccatgatcgc ggcggccctg gtggcgttcg
    60541 tcgggctgct ggcgctggcc ctgaaccagg agcgattgcg ccggctcacc gggctattgg
    60601 cccgggtcat gccgcaccgg atcgccgacc gggtggcctc catcgcctcg gggttcgcct
    60661 ccggcgcggc cgccacgcgg cggcccgcga ccctggccgc ggcggcgggc ctgtccctgg
    60721 ccatctggac gatcggcggc acggcgacgc tgctgtgggt gcgcgccttc aatttcgata
    60781 cgccctggtt cgccggcttc ctcgtccttg tggtggtcaa cctcggttcg gcgatcccat
    60841 cgtcaccggg ctacgtgggc gtctaccacg cggccgcgat cctggccttg agcctgtggg
    60901 tgcccaaggg gccggccctc gcttacgcgt tggtcaccca cgcgctgaac atgctcgcga
    60961 acgtggtcct tggctcctac ttcctcgtcc gcgagggcgt aagcctgaag ggaattgagg
    61021 aacaggaggt aacggagtga acagaggggg agtgttgggc tcaattttcc ctgcgaactc
    61081 ctgtgcaagc gagcaacggt atggacggat tcaataacgg ttccaggcgg aggttgggct
    61141 ggggggttgt gctgggcgtg ctgctgctcc aggtctggtc ccgctatctg ccctcgccct
    61201 gggccgtgtt cgtggcggac gactggacca actacgcccg cagcacgctg ttcccctcga
    61261 tgaaggaggc cgtcacggtc agcctccagg acccgaaccg gccgttgagc accacggccg
    61321 tggccgtcgg gtatcgcctg ttcgggccgc ggccggtgta ctggacgatc ctgtcgctgc
    61381 tgtcgcattc gctgatgctc tggttcatca tgaagatggg cttggagctg accgggagcc
    61441 gccgcgtggc cgcgctccag ggcgccttgt tcgccctgct gcccaacctg cacgagacgt
    61501 ggcagtggtc cacccagatc ctcaacgagg tgacgtgcgg cctgctgttc tatgccctcg
    61561 ccggctggct gttcgtggcc cacgtccggc gcggcggggc ggcctggctg gcgggcgcgg
    61621 cgctggccta cgccgtcggc ctgttctcgt acgagcaggg catcctgctg cccgccgtcg
    61681 gcctggcgat cctgccatgg cgccggtcgc tcgggcgcgc gctcctgaaa atggttccgt
    61741 tcggcgcggc cttcctgctg tacgcggcat ggcgggcgac cgattcgttc ggcctgaaca
    61801 cggcctggca ctaccccgac cagatgaagg tcggcttctc cctgtggggg atcggctgga
    61861 acgcccggca gctcgtccac tggtggatcg gcgaccggtt cttcggcgcg atcctgtccg
    61921 ggctgatgag cttctccacg ctggccccgt ggacgcggcg gctgctggcg ctgggcaata
    61981 tcgctgcgat ttccgcggcc gtcttcctgt ggcggcgcct ggcttcggat aaagaggacg
    62041 cgctgcccgc ggccttttcc gcggcgcagg tcgcgggctt cggcgtgctc tgggccggcg
    62101 cctcgatggc cacgatcctc gcgtcgtaca cggcgggccg gctcaacatc ctgcccgcga
    62161 tggggctgac gcttctcggc ggcctgctgc tggcccgcct gccgtggtgc gcgtgggcga
    62221 ccgcgctctt cgtgccggcc ctggtcatcc tggcggccaa ccaagggacc tcggaaaatt
    62281 ttcggcaggc cggcgaattc aactggcgcg tgtacgacta cctgcgcgtc cgccaggcgg
    62341 aatggcagga caaggacgtg ctcctgttcg atacgggctc cctccgccac cggcagacgc
    62401 ggggcctggt gacgccggcg tcggcgctcg agtacacctg ggccgagtac gggaacacgc
    62461 tgctcttccg cggcttcgtg ccgttgggca tggtgcagcg gctcgccggg gcgatgtccc
    62521 cgcatgtgca agtgatccaa gacgtcgagt gcggggcgcg cgtcgacggg agcgaactgg
    62581 cctggcacac ccggtaccgt cccgacgagc ccttccggac gccgctggac cgcgtgttta
    62641 tcgtcgacgt gttccaggcg gcccaatccg tgccgccccg ccctccgctg tcgccgcttc
    62701 catgaagatt gcgtaccttt gcgagccgca ggccggcggc acgttcacgt tcttccagcg
    62761 cgtgcggccg cgcctggccg cgctcggcgt ggacttccgg tgcgtgccgc cgtattccgt
    62821 cgagaccttc gccggctcgc ggttcgaggg cctggaaggc gtggattttc tggaactccc
    62881 ggacggcctg ccggacgcca cgcgcgccct ggtccggcac ttggaactgc aggattatcg
    62941 cggcgcgctg atcctgccgg gctgcgacgt cctgacgacg aacctggcgc ggtacctgcc
    63001 gcgcggcgtg cggtgcgcgg cccgcgtggc gctgatggcg cgcagcgcct acgtgtccgc
    63061 ccgcgcggtc gcaggacacc tgaacgccgt gttcgcggtc agccaccgcg tggcccatga
    63121 cctcgaacac tcctatggct tcccggcggg atcggttcgc attgcctaca acggcgcggt
    63181 gctccggccg ctgccggcgc gggacccggg ccgccgcccg ttccggctga tctacaccgg
    63241 gcgcctgtcc gacctggaca agggcacctt gatgttgccc gcgatcctgc ggcaggcgct
    63301 cgacgccggc gccgatgtct tcctcaccgc cgtcgggaac gggccggatg aacagcggtt
    63361 gcgggagggc tttcgccggc tcggcttgga agatcgggtc gaattgctcg ggaaccagcc
    63421 gctcgatcgc gtggaccggc tcctcgacga gagcgacggc ttcctgctgc cgtcgcggct
    63481 ggaggcctgc cccaacgcga tgctccaggc gatggccgcg ggttgcgcgc cggtggcctt
    63541 ccggatacac gactccgtgg accgcatcgt ggaggacggc gtcagcggcc tgctggcgcc
    63601 ggtcggggac gtcgccgctt tcgcgcaggc cgtcgtgaaa ctggcgggcg accgggccct
    63661 gtgcgcccgg atgagcgcgg ccgcgcggaa gcgcatcgag gagcatttct ccgcggagcg
    63721 cacggcacag ctctacgccg aggggtttcg cgccatgctc gacgggcccg accgccgcgc
    63781 gccggcgctg tcgctggacc ggtacgaagt cccgcgcgaa ctgaagccga cctggcgcac
    63841 gctgatcccg gcgccggtca agaacatgct gcggaagtgg atggaacggt tcgggcgaac
    63901 ggcatgacga gggagcagga ggccacagag ataacggagg ggatcgggac tctctgttcc
    63961 ctccgttgcc acctgttcaa atacgcatga aggttttgtt catcaatcgc atgttgagcc
    64021 tggtccgggg cgggggggag acgttcgacc tcgaaatcgg gcgacacctc gcgaagctgg
    64081 gctgcgaggt gtcgtatctt tccggcctgc ccctgttcag ccgcggccgc ccgccggtga
    64141 tccccgcgtc cttccagccg ccgccggcaa tgcacacggt ccggtcgccc tggctggcgt
    64201 ggattccgtg ggaccgcatg cggggcggct ggcgggtatg gctgctggac cgccggctct
    64261 tcgaggcgcg cgcgttgaaa tgggtgctgc cgcgccaggc ggagtacgac atcgtgcacc
    64321 tctgcgagat gcccgactgg cccgcggcgg cgaagcgcgc gggcttgaaa atcccggtcg
    64381 tcgtccgcct caccggcccg gagggggcga aggattcccc gggcctgcgc gccacggacg
    64441 gcgtgatcgc cagcggcgtg tcggtggaga aaatccggcg cctcgtgcgc ccggactgcg
    64501 agaacatccc gaactcggtg gacacggacc tgttccgccc gcagccctcg gatttccgcc
    64561 ggcggcttgg aatcggaccc gatacctgcc tggtcctgta cgtcgcccgg ttccaggact
    64621 tcaagaatca ccagatgctg gtccgcgcct tcgcggaatt tctcaaggag cggcccgatg
    64681 cgcgcctgat cctcgtgggc gaggggcacc tgaagtcctg ggtccagaaa gactgcgcgg
    64741 aactgggcat cgccgaccgc gtattgttcc tcggcgaagt gccgtttacg gagctgccgg
    64801 ccctctatgc ggcggtggac atcaaggtca tctccagcga ctacgaatcg ttttccttcg
    64861 cgaccatcga ggccatggcc tccaggctgc cgattgccac gaccgattgc gcctgggtgc
    64921 cggttctcat cggcgactct tcttcgtcct ccacccttca tccctcatcc ctccgcctcg
    64981 cccccggcgg ggtgatctct cccatcaagg atcccggcgc gctggcgcag gggctgctcc
    65041 ggttggccgg caacccggag cttcggaagc aaatgggcga atggaaccgg cgcaaggccg
    65101 tggccgagca cgggtgggcg gcgagcgcgg cgaagctgct ggcgttgtac cggcgattga
    65161 cggggaagga gtcgtgatat gaacggtatc cgcggattgc tcaagcggtt ctggtcccgg
    65221 cggcaggccg aggtgcagaa aaagtggaag cggacgctgc ccttcggcga ctacgtcgtc
    65281 gaccgctggg aaaaagcccg gctgctgggc ttcggcgagg gcgccagcat ctacgacagc
    65341 gcgatcgtca tgggcgatgt ccaggtgggc gcccgcacat ggatcgggcc gggcgtcatc
    65401 ctggacggct cgggcggcct ggccatcggc gactactgct cgatctcggc cggcgtgcag
    65461 atttacacgc acgacagcgt ggactgggcg gtcagcggcg ggcagaagga aatcgtgcgg
    65521 caacccacgc gcatcggctc gcgctgctac ctcggcccga acgtcgttgt cgccaagggc
    65581 gtgaccatcg gcgacggctg catcatcggc gcgaacagcc tcgtcctcca cgacatcccc
    65641 gccggccaca aggcgtacgg cacgccgtgc aaggtggtcg gcccggtcgg ttgagtcggt
    65701 agcggcggct tggacatgaa cgaagctctc ctgctgctcc tggtcctggc ggcggtgctg
    65761 acggcgcgcc tctggccgtc gtggcgtcat ccgcagatcg gctcggacgc gggctttcat
    65821 ctgctcctgc gccgcgagat ccggcgcggc gggttcaggc cgcccgcccg gctggaggcc
    65881 tgcctcttcg accggaccgt cggctacccg tggttgtacc accagttcat agcgctcttc
    65941 ccggagtcgt ggctgcgtcg cttgccggcc ctgccgagcg cgctggcgga tgccgcgcag
    66001 accgggctgg tgttcctagc cggccgcgcg ctggccgggc ggttcgcgcc gggcgccgat
    66061 ccggcctgga cgggtttcgt gtgcgccgcg ctctacgggt tgaatcccgc gatgctggcg
    66121 cacggggtcg gcccgcgcgc cttcgagatc acgccgcgcc cgttcggtga attgctgttc
    66181 acgatctcgc tcgggagcgc tctcctggcc gcgtatctgt cctcctgggc ctgggcggcc
    66241 gtctcggtcc tggccggcgc cggggtcctg ctgtccagca agttcgccgt ccaggtgatg
    66301 ctgttcttca tgcccttgct gtccttcctg ccgggactgc accggttgtg gctggtgcct
    66361 ctggcctctc tccccgtggc gttagcgcta tccgccgggc gatatcggca tgtcctgcgc
    66421 ggccaggtcc ggcacctcga ttacttccgc cgcaagctgc agtatacttt cggcacggtc
    66481 gccaaccgta accgctggca ggatgtgcgg ttgctggccg cgacggtccg gggacacggt
    66541 ctcttctcgc gggccgcggc ctccgcggcg tccgacttct atttccataa cacgtacctg
    66601 cagatggcga cgcgcggcgt gctgttcctc ctgctcgccg tgctgtgcgc gggcggttcg
    66661 ttccgcggaa tcctgctcta ccgttggctg ctgggctggg cctgggtctg ggtgctgccc
    66721 ttcctgctca cctcgtggcg ccacgcccgg ttcctcggcg aggccgagcg ctacgcgggg
    66781 tacagcatcg cgccggccgc gctgctggtc ggcctggggc tggtctcccg gccggtctcc
    66841 ggtctcttgg ccgccggctt ggttctctat gcgctggtca cgatcgtgat cttcgcccat
    66901 gcgctccggt cgcatgcccg gtcggcgggg ctcgtgcggg acgcgcggcg cgagctggcc
    66961 gggttcctgg gcggactgcc cgccggcgcc gtgctgctcg gcgcgccgga ggaactggtg
    67021 ctgggccccg tcgcctcgct gctgccgcac cggttcgcgg acatcatggc ggacatggac
    67081 ctgttcgtcg agaagctgga gcaccattgc gacggctatc cctggccgcg ggcggactgg
    67141 gcgttctggc gggcggcggg cgtgcagtac gtggtcgccc ggcaccccga cgagatccgc
    67201 gccgtgcggc ccgacctgaa gtacgatttc gcctcgctcg aacggctgtt ctccaacgac
    67261 gcgtacgacg tctaccggct ctgaccccat gtgcggcatt gcaggattca tcggcgaagg
    67321 actcggcctg accccggaac ggctggcccg gatgcgggac gcgatcgcgc accgcgggcc
    67381 cgacgaccac gggctgaagg cgtggaccgc cgcgggggag gacgccgcgc cggacgcggt
    67441 cgccgccgcc ggcctggccc accggcgctt gagcatcatc gacctgtccc cggccggccg
    67501 ccagccgatg tccaacgagg acggcacggc ctggatcgtc ttcaacggcg agttctacaa
    67561 cttcggcgac tatcgcgcgg agctcgaggc gcagggccac gtcttccggt cgcattccga
    67621 cacggaaacg atcctgcacc tctacgagca gcacgggatc gaggagaccc tgcgccggat
    67681 caacgggatg ttcgccctcg cgctctggga cgcgcgccgc tgccggctca tcctcgcccg
    67741 cgaccggctg ggcaagaagc cgctcttcta cgcgtggcag cccggccagg gactcgcctt
    67801 cgcctcggag atcaaggccc tccgggcggg cggcctcgtg gacgacgcgc ggctggacct
    67861 cgtggcgctg gaccaggtct ggaccttcgg cgtcaccgtc ggcgagcgca cgctcttcga
    67921 gcaggtccgg aagctgccgc cggcgcgcta cgcggtgttc gagaacggga agctggagca
    67981 gcgcgagtac tgggacgccg tcttccgcgg cgcgccggcc gacaaccggg cggacggcga
    68041 ctgggtcgac gagctcgacg cgctgctccg cgacgcgatc cgcctgcgcc tggtctccga
    68101 cgtgccggtc ggcctgttcc tctcgggcgg cgtggactcc tccctgatcg ccgcgctcgc
    68161 cgccgacatc acgggcgggc ggatccattc ctacaccatc gggttccggg aggacgcctt
    68221 caacgaggcc ccgctcgccc gcgccgtcgc ggagcacctg gggctgaaga acgaaacgct
    68281 ctgcgtcacg gacgacctct tcggcgaggc cggccgcatt gcgcgccagt tcgacgagcc
    68341 cttcggcgat tcgtccgccg tgccgaccta ttacgtctcc aagctgtccc ggaagttcgt
    68401 caccgtggcg ctgaccggcg acggcggcga cgagtcgttc gccgggtacg actacatccg
    68461 ccaggccgcg cgcatgtggg gctccgcgga gcaccggcgg cggctgcggc gtccgatggg
    68521 cctgcgcgag cggctctggg aattcaagca gcggctgctg gggttcgagc gcgggttcct
    68581 gaacctggac agccagctct cgcggaagcg ccgcctcgag ctgttcacgc cggaattcct
    68641 gcgcggcgtg ccgcacggcg aggccgtcgc ggaccgcgcc cgctggatgg cgcggaccgc
    68701 cggggccgac gtgttgagcc gggtgcagta cctgctcgtg aagacctggc tgccggacga
    68761 ttttctgcgg aaggtcgata cggtcagcat ggcgcacgcc ctggagtgcc ggagtccgct
    68821 gctggattac cgggtcgtgg agttcgccgc gcgcctgccc gaccgcctga agttcgagcc
    68881 ggaggggcgc ggcaagcgga tcctccggct tctgctccag cgctacgtgc ccgaggaatt
    68941 gtacgaccgg cccaagcggg gattcgggat cccgtgggag cactggtgcc ggggcgaggc
    69001 cgggcgccgc ctgaaggcgc gctgggagca gtgggcgaac ccgtggttcc ggcccgaggc
    69061 ggccgcgatg ctgttcccgg aggaccgcgt cggtgtgaac ttcctacaat ggacggcctt
    69121 tgctacattg gagcacttcc ggccggaggc cggcgcatga aacagggcat caaagaatgg
    69181 atcgcgggct cgggcctgcg ccggctcgtg aacacgtggc tgggcgggaa ccgttatgcc
    69241 gtgcgcggat cgggcaaccg gctggactgc tcgcgcggcc tgctgacccg cgttcgcgtc
    69301 tgcataggcg ggaacgacaa ccggatcgag gtgggcgagg gggcgcgcct ggcgaacctg
    69361 gacatcctgg tccgcggcga cggccaccgg ctgtccatcg gcgcgggctg ctcgatcgcg
    69421 gccggcaaga tcaagctgga ggaccgtggc agcgcgatgg agatcggcgc cggcacgacc
    69481 atcgaggacg cctacctggg cgcgtacgag ggcgcgcgag tcgtcctggg cgcggattgc
    69541 atgttgtccg aggccgtcgg gctgcgcagc ggcgacatgc actccgtgct ggacgcggcg
    69601 accggcgcgc ggctgaaccc ggcgaaggac atccgcctgg gcgaccacgt ctggctgggc
    69661 cgcggcgtca cggtgctcaa gggcgcggcc atcggcgacc gcgcggtcgt cggggcgcag
    69721 gcgctggtga ccggggatat cccggccggc agcctggcgg tcggcgcgcc ggcgcgggtc
    69781 atccggagcg gcgtcacctg ggaccgtcgt cggctggcgg taacggaaga acggaagaaa
    69841 gaagagatag acaggcggta acggagggga tccgaaatct ctgttctctc cgttgactcc
    69901 tgttccattt tcgcaaatgc tcttcaacag ctacgagttc ctgttcctct tcctgcccgc
    69961 ggtcctgctg ggctactacg tgctgctccc gaagcgcgcg cgcctgctgt tcctgaccgt
    70021 cacgagctac ctgttctacg gctggtggga ctaccggttc tgcgcgctga tgctgctgtc
    70081 caccacgatc gattacgcgg ccggccgcgg catcggcgcc gcgaagacgc cccgcgtccg
    70141 ccgtcgctgg ctggtcgcgt ccatcgtctc cggcctgtcc ctgctgggtt ttttcaagta
    70201 cttcgacctg gcggcgcgga cggcgaacca tgtcgcctca ttcctcggcg ctccggaacc
    70261 gatcctgccg ctgctccaca tcgtgctgcc ggtcggcatc tcgttctaca cgttccaaag
    70321 catgagctac gcgatcgaca tctacctggg catggcccgg ccggcgcgca ccttcgtcga
    70381 ttttgcctgc ttcgtagccc tgtacccgca gttgatcgcg gggccgatcg tgcggtaccg
    70441 cgatctggcg gagcagctca tcgagcggac gcatacgctg gccaagttct cgctgggcgt
    70501 cgcattcttc gtgctcggac tggccaagaa ggtcctgctg gccgacggcg tggcgacgct
    70561 cgtcgcgccc gccttcgagc aggcggcgcc cggcctgttc acggcctgga cggggatgct
    70621 gggctacgcg atgcagatct attttgactt cagcggctac tcggacatgg cggtcggcct
    70681 cggcctgatg ctgggcttcc gcatgccgca gaatttcaat tcgccgtacc aggccgtgtc
    70741 catcaccgat ttctggcggc gctggcacat cacgctgtcg agctggctgc gcgactacct
    70801 ctatatcccg ctgggcggca accggctcgg gcccgtgcgg acctacatca acctgttcct
    70861 gaccatgctc ctcggcggcc tgtggcacgg cgccaactgg acattcgtcg tgtggggcgg
    70921 ctaccacggc atcctgctgg ccgtcgagcg ggcggccggc aagaaaggac tgttgagctg
    70981 ggcgccgccg gccgtccagc gcgcgctgac gttcctgctc gtgctgctcg gctgggtgtt
    71041 cttccgcgcg acgtcgatgg gggaggcggg cgcgatgttc aagggcctgt tcggcgtcca
    71101 cggactcggt cacgggatcc gggccgccct cggcgcggcg cccctgccct atctcatgct
    71161 ggccgtgacg atggtcctgg ctttcggatt ccggaacacg tgggagatcc ggtggcggaa
    71221 cggttggcgg ctggcgctcg ccctggccgt gctgtatgcg gtgtgcgtgg gggcgatcct
    71281 ggtgaatacg agttcgccgt tcctctattt ccaattctga cgccgggcat ggcatggaaa
    71341 cgctgaacga caaagagcag acctgggcgg aaggccggag ctggcgcggg gcggtcctcg
    71401 ccgtcgtcgg cctggcctgg cttgcgggcc tgggcttcga ctggatgctc cgcttcacgt
    71461 ggtacggctg gcagcagcgg ctcatcttcc ggccgtcggc ggtccggacc ctgaaccagt
    71521 cgagcgatcg gtatgtcacc aacgaggtcc ccgggatccg cggcggcgac ctgacgcggc
    71581 tggtgggcat ccggtcgttc aacgcgcgct tcgagcagga ccatccgggc ggcccgcaga
    71641 cctgcgacgg ggcgggattc cggaacctgc ccgccccgcc ggacctggcg cacccggtcg
    71701 tcgtggtcgg cgacagcttc atggccgccg gcgatcccat gaccaacatg ttcgccgtgc
    71761 aactcgcgga gcggtccgtc ctgccggtct acaatcgcgc catggtcggc gtcggcccgt
    71821 tcctgagcgt cgagcggttc gtggacagcg accggttcca gcaccgccgg ccgaagatcc
    71881 tggtctgggg cttcgtcgag cggtcggtca tccagcacgg cttctccagc ctgctgtacc
    71941 agctggacat ccgcgacaag ggcctgaagc cggcggagaa ctatatcctt tcggccacga
    72001 cgacgcgcgt ggtatggcag gccttgttgc ccgggaacct caagaccgcg ctgcccgaca
    72061 cgtctgcgct cgcccagctt tcgcagcggg cctggaaccg gatccggtac gcgctgttcg
    72121 gcaagatcat gcccgaggtg ttcgccgccg cgggcgacgt ggccggaaag ccgatccttt
    72181 tctaccggcc tgcgttggac ctgtcgctgg gcgacccgtt cgagcgcgat cccgatcaga
    72241 tcatggaggg catccgctac acgagcgatt tcatggcgcg ccgcggcatc cgtctcgtga
    72301 tcctgatgat ccccgacaag gaaacgacgt accgggagcg gatcccgccg gccgaactcg
    72361 ggggccggga actgccgccg tcctgcctgc tcgagatcga gcgccggctg cgggcggaga
    72421 acatcccggt ggtcaacctg gtcgagccgt tccgggagcg cgccgcgcgc ggcgagctgc
    72481 tctactggcc ggatgacacg cactggaaca gcgcgggcat ccggatcgcg gccgagctgg
    72541 tctggcgcga gctgcaatcc ctgcccgaga cacaggcgct gctcaagcat gagcccccat
    72601 cggatccact tcaatagccc ccgcgccgcc gggcgcgagc tggcgaacat ggcccgggcc
    72661 ctggaatccg ggcacatctc cggcgacggc gagttcaccc ggaagtgccg cgacctcctg
    72721 aagcaggagc tgggcgcgac cgacgtcctg ctgaccacgt cctgcacgca cgcgctggag
    72781 atgacggcgc tgctgctgga gctcaagccg ggcgacgagg tgatcgtccc ctccttctcc
    72841 ttcgtttcca cggccaacgc ctttgcgctg cacggggccc ggcctgtctt cgcggacgtc
    72901 cggcccgaca cgctgaacct ggacgagcgg cggctggagg agctgattac gccgcgcgcg
    72961 cgcgcggtcg tcgtggtcca ctacgccggc gtcgggtgcg aaatggacgc catcctggcg
    73021 gcggcccggg cccgcggggt ggcggtgatc gaggacaacg cccacggcct gttcgggaaa
    73081 taccgcggcc gctggctggg cacgttcggc gcggcggcga cgcagagctt ccacgagacc
    73141 aagaacttca cctgcggcga gggcggcgcc ctgctggtca acgagccccg ctggcgggcg
    73201 cgcgccgaga tcctgcgcga gaagggcacg gaccgcggcc ggttcttccg cggcgaggtg
    73261 gacaagtact cctgggtgga catcgggtcg agctacctgc cgtccgacct gcttgcggcg
    73321 ttcctgctgg cccagctcga acagcgcgcg gccatccagg cgaagcgcaa ggccctgtgg
    73381 cacgggtacc gggacggcct cgcggactgg gccgcggcgc gcggcgcgca attgccggtg
    73441 atccccgcgc attgcgaatc ggcgtatgcc ctgttccacc tgctgctgcc ctcggcggag
    73501 gaacgggacg ccctgatggc gcatctcaag gcgcgcggga tttcgagcgt gttccactac
    73561 ctgccgctgc acctgtcgaa aatggggcgg accttcggcg ggcagccggg ccaatgcccc
    73621 gtgacggaat ccgtgtgcga ccggctgctg cggctgccct tccacaacgg cctggccgag
    73681 gccaaccagg cggaagtcat cgaggcgatg cgggccttca ggcccggctg acgcttccgc
    73741 tcttccagct tttcttttcc cggtgcagcc ccgccaggtc gatggcgcgg gccagtccgt
    73801 acgcgaagta caggacgcac aacgccggga agtgccggaa actccctccc ttgcggcagg
    73861 tccatgccgc cggccccgcg accagcacgg gcaggggcaa cgcgagcagc cacccgctcc
    73921 agtgcccgag ccatcccgcc gggaccgcca gcgcgaaaag cagcagcgac gcggcgaaaa
    73981 gcctcgcgaa gcgcggggcg ttgccgcccg cccgctggcc gctcccgtcc acgcgggagt
    74041 agctcaaccg gttggcgtgc cagagctgct gccggaaaaa cgcccggagc gtcgccggtt
    74101 cgccgtggtg caccacgcgc agcgccggga cgcccagcac cgggatgccc gcccggtgcg
    74161 cccggaaggc gaagtcggtg tcctcgccgg tggccagccg ctcgtcgaat ccgcccaggg
    74221 cctcggccgc gctgcgggtc agccacatgt tccgggtcgt tacgagccgg aaggcctcgc
    74281 gccgcacgac ctcgccgcgc gcggattgct cgatggcccg gttcttgtgc tgccagtgaa
    74341 aatgccacgc cgcctgcacc cacgtcatcg gcgcgggcgg ctccaccggc cagccgagga
    74401 tcacgcgcgg ttcgtgttcg agctgcgccg ccgcctgctc cgcccagtcc gccgccggct
    74461 cgcagtcggc gtcgaggaac gcgatccact cgccggaaga ctccttcagc ccgatattcc
    74521 ggcacacggg gatgctcgcg ccgggcgcat cgagcacgcg gtcgaatccc cgttcccgcg
    74581 cggcttgcgc cgtgccgtcc cggctgccgc cgtccacaag cacggtctcg atctcgcagc
    74641 cggccggccg cttcaggcgg gcgatggacg gctgaagccg gggcaggttg ggcgcttcgt
    74701 tgaatgcgac aatgatgatg gaaaggcggc ggcccatgcc ccggatgtat aacggttacg
    74761 ggtggactct gcacgcggaa aaggcagaat ggccggcatg aaacacgtcg gcatggtgct
    74821 ggcagcgggc gcgtcgtccc ggatgggacg gccgaaggcc ctgctgccgg cggagggcgg
    74881 gcggccgctg gcttgtgtcc aggccggcct gctgcgcgcc ggcggctgcg cccgcgtggt
    74941 ggtggtcctc ggcgcggatt tcgaccgtat cgccccgcaa ctggccggcg aggaagtcgt
    75001 ccggaacccg gaatgggaga aggggcgctt cacctccgtc cagaccggcc tgcgcgcgct
    75061 cggggattcg gacggttgcg tcatcctgcc cgtggatgcc gtcggactgc gcgtggaaac
    75121 cgtccgcgcc gtcctggccc gggctgaaac cgggatcgcg ccggcggtcc gcccgacctg
    75181 gcgcgggcag gacggcaagc tgctgtggat atcccgggcg ctggcggaag aatggcggcg
    75241 cgagccggcc ggggatcagc ggctcgacga ccgggtccgg gcccgggtcg agcgcgtggc
    75301 cgtggacgat cccgccctgt tgagcaatgt gaatacgccg gaagatcttt gacagccgcc
    75361 cggtctgcgt tatcgtgggc cggttgcggt caacctcaaa ggagtgtcat catgggtatg
    75421 atcaaggaat tcaaggagtt cgcggtcaag ggcaacatga tcgacatggc cgtcggcatc
    75481 atcatcggcg gcgcgttcgg caagatcgta tcgtcgctgg tcaacgacat cctgatgccg
    75541 cccctggcta tgctgatggg cggcatcaac ttctcgaaca aggtgatcac cttgaaggag
    75601 gctgtcggtg aagcgccggc cgtccagctc aagtacgggc tgttcatcaa cacggcgatc
    75661 gatgtcgtca tcgtggccct cgcggtcttc atgatggtca aggcgatcaa caggctgaag
    75721 aggaaagagg aggccaagcc ggccccggcg cccgcgatct cggacgaggt gaaactgctc
    75781 acggaaatcc gcgacgcgct gaagccgcgt tgaggccgga tcggaagccg tgaatcccgt
    75841 cgaggcaggc ccggcgtcga atcgaatccg gtggatggcc aacgccgccg tgttcgtgtc
    75901 cggcgcgtgc atcatgatcg tggaactgct ggccgggcgc gtgatttcac ggcacgtggg
    75961 gcagtcgctt tatacctgga ccgcgatcat cggcgtcgtc ctggccggga tcttggcggg
    76021 caactatgcc gggggaatgc tggcggatcg ccggccgccc cgccgccttc tcgcgctcct
    76081 gttcctgctg gccgccgcgg gctgcttcgc cgtgcgcggg ctgaacgacc tggcgggcca
    76141 ctggtccctg ctccggggcc aggcctggcc cgcgcggatc gtccttcacg tgctgatcac
    76201 gttcctgctg ccggcgctgc tgctggggac gatcgggccg gtggccgccc gcatggcggt
    76261 cggggccgac ccgcggaccg gccgggcggt gggcgacgtc tatgcctggg gcagcctcgg
    76321 cgccatcctg ggcacgttcc ttgccggctt cgtgctgatc ccatccttcg gcgtcacggc
    76381 gatcgtcggc ggcgtctccg gcctgctggc cgttacggga ttgctgtatg ccgcggcgtc
    76441 gctacactga ttcccgatga agatcgccgt catcggggat ggggggtggg gcacggccct
    76501 ggcgctgatc ctggccggca acgggcacga ggtctccgtc tgggggcctt tcgaagacta
    76561 tctccggacc attcgcgaaa cgggcgaaaa caaaaagttt ctgcccggcg tgcgcctgcc
    76621 ttccgcgctc cgctggaccg cccggctcga cgaggccgcg gcgggggcgg aggcggtggt
    76681 gctggcgacg ccgtcccgct ttttccgcgg cgccgtttcg gcgttggcgc ccttcctgcc
    76741 gaacccccgc ccgctgatcg taagcgtcac gaaagggctg gacgagaaga cgcaccggcg
    76801 gatgaccgag gtggccggcg aactgctcgg cggggaggcg gtcgcggcgc tgtccgggcc
    76861 cagcctggcc gccgaggtcg cgcgcggcgt gccgaccgcg gtgacgattg cgtgcgcgga
    76921 catgggccgc gcggccgccc tgcagctgct gttcaacggg ccgcggttcc gggtgtacac
    76981 gtcggacgac gtggtcgggg tggaactcgg cggcgcgctg aagaacgtca tcgccatcgc
    77041 ggcgggcgtc tgcgacggga tcgggttcgg cgacaacgcg aaggcggccc tgatcacgcg
    77101 cggcctggcg gagatcgggc ggctgggcgt ggcgatgggc gcgcatccgc agaccttcgc
    77161 gggattgagc gggttgggag acctggtcgt gacctgcgcc agccgcttga gccgcaaccg
    77221 cggcgtgggc gaacggctcg gccgcggcga atccatggag gccatcctgc aaggcatgca
    77281 gcaggtggcc gaaggcgtgt ggacctgcgg ggcggcgcgc gatctggccc gaagccggaa
    77341 cgtggaagtg cctatcacgg aagaagtttg cgcggtcgtg cacgagggca agaatccgcg
    77401 ccgggcggtc gaggatctca tgagccgcga tccccgcccg gagcgcgaat gagccgggtc
    77461 gagcggcttt tcggaaagcc ctgacttttt cccttgccac caaaatcgat tcagcctatt
    77521 ttcacgtgac ttttcagtcc ggagttatcc ttgtttgcag ccagcccaaa aaggagaagt
    77581 gcaatgatgt cggtcaatcg ttttagcgcg gtgccgctgg tagtgctgat ggttttctgc
    77641 gcagcgactg cttggtcttc cgacttgggg acggatagtg ccgccaacta cacgtcttgg
    77701 accaatggaa gcagtgcggg taccggcttt caggcatggg aactgaccac atggcttcag
    77761 tatgccgggc acttcctggg tacctccacg aacctcggca ccaatgtgaa cattgacacc
    77821 gacggcaagg cattcggtat gtatgccaat ccgcccgaat ccaacagcta cgcgaacgcc
    77881 acccggtatt tttccggtgg agcgttgacc gagggacagg ttttttccat cgatctggcg
    77941 gtgaaccact ggaatggaaa caagggcatc gatatttaca gcgcgggggg tgccaagctg
    78001 ctgaacatca acttcagcga caacaacggc gggttgaaag tggagtatgg aatcacgacc
    78061 aacatcctcg ggtggtccaa ctcgggcgcc ggctacaact atacgcttga agtcgagaag
    78121 aaggcgggaa ccacatcccg ggtcagtctg cagcgaaacg tggacggcag caactatgtg
    78181 gcggaactcg atctcggaaa cgcgccgggc ggattccact tgtactcggg cgcgacagaa
    78241 gctggagctc ccaacgattt attcctgaat aacatgagtc tggcggacag cggaacgccc
    78301 gtgctgaccc tggtcggccg cgcggctatg ccggcgaatt tcaccaaccg gctcacggtc
    78361 acgcgcaacg gctcgacggc ggccgcgctg gacgtcacgg cgtggagcgg cgacgaggcc
    78421 gtggccacgg tggacaccaa cgtgacgatc gagatcggca gcaactcggc gcccgtcgag
    78481 atcgtcggcg tggacaccgg cacggccttg atccaggcca cagcggacgg cttcccgacg
    78541 gcctcgctct ccgtggcggt cttcgacgtg gcgtacgacg attcgtccta ctatccctcg
    78601 ggcggctgga ccagcggcgc gaaccagggc agcggcttcg cggcctggat catcaccaat
    78661 aacaacggcg agggcgtggg gttcaccaac tacgccggca gtttcatcgg caattccacg
    78721 gccgacggcg gcggccacgt caacgcccag cccaccggcg acgcgttcgg cctctatgcc
    78781 aaccagaacg ggacgggcgg cgaccccctc tcgcaggcca cccgcccgtt tgcggcggaa
    78841 ctcgccgcgg gcgacacgct ctccgtggac ctgggcgtga acttccgcga tggctccaag
    78901 ggcgtcatga tccagagcgg cgacacgtgg ctgctcgagg tcgcggtcat cggcgacgat
    78961 tacgtctacc agaaccacgg cggcggcggc gcgcagacca gcctcggctg gacctacgag
    79021 gcgaacacct cgatccgcgt ggaggtgacg cgcaagacgt cgttcctgta cgacgtcagc
    79081 atcatccgcg gcgggacgca gaccgcgacg aacaagctcg ccggcgtgaa cctcggcggc
    79141 gcgccggacc gcgtccggtt ctacaacttc aacaccgcgg gcggcggcgg caacaacctc
    79201 ttcttcaacc ggctggcgat ctggagcggc gcagagaccc cggtcctgtc cctcgcgggc
    79261 caggagggca tggtggtgaa ccataccaac aagctggtga tcagccgaac gggggaaatc
    79321 ggcgaggtct tggatgtgaa cctggccagc gacgacacca acgtggtcgt ggtccccgcc
    79381 tccgtgcaga tcagcgacgg cgagacgagc gcctccgtgg acatcgtcgg cgccggccgc
    79441 ggccaggcga cgatcaccgt ctccgcggcc ggcttcgcgg acgtcacggc ggatgtcgag
    79501 gtgtacaacg tggcttacga ttccacctcc tactatgggc cgggcctgtt cacgaacggg
    79561 gcgaacggcg gcgtcgggtt cgacgcgtgg accatcagcc acaacgacgg cggccatgac
    79621 gggtatacca acttcgccgg cgccttcctg ggcgactcga cggcctccgg cggcggggac
    79681 gtcaacgcgg tctccaacaa cgccttcggc ttcttcgcga accaggtcgg tgacgacacg
    79741 ggaaccaacg aaccgatcgt cgaagccacc cgcgggttca cagccctcgc catgggccag
    79801 agcctgaccc tggacctggg cgtgaacttc cgcaacgggg cgaagggcat gatgctccag
    79861 aacggcgaca cctggctgtt cgaggtggtc gtcacggcgg accagtacgc gtggcagaat
    79921 cacgcgctcg gcggggccct gcaggacctg ggctgggttt atgcggcgga ctcggccatc
    79981 aatgtcacgg tcacccgagt ggggcaggac aagtatgaca tcgggctgac ccgttcgggt
    80041 tcggcccccg agtacatgct gctggagggc gtgagcttca accatcaggc gccggaccgg
    80101 gcccggttct acgcctggaa caccgagcag ggcggcgaga acaatctcta tgtcaaccgc
    80161 ctcggcatca acgagttcga agcgctcgag atggagggcg tgatcaatat cgacgtcggc
    80221 ggcacgacaa cgatcttcat ccagcgcagc gacacgaccg gcccgctgac cgtggacctg
    80281 tcgagcgcgg cgccctccgt ggtcaccgtg cccgcgacgg tcgagttcgc ggatggcgac
    80341 gccgtgacga gcttcgtcgc caacggcgtc tccgagagct taaccacgat ccatgccgag
    80401 gccgatgggt acgtcggcgc gcccctgctg gtcaacgtgt tcgacttccc gggcgagttt
    80461 gacaacgccg gcaactatgt cccgggcctg atcgtaccga cgaccttcac gaatgagtcc
    80521 aatggcggcg caggcttcgg cgcatggcag ttctctgccc tgtcggacgg tgcggccatc
    80581 cagttgacgg attcgagggt gtcgaccgcc tggatgaatt cgttcaacga ccggtccttc
    80641 agcttcatcg gcgggacgaa gtcgaactac gcctcggcct accgcgacct gaacaccgcg
    80701 ttggcggaag gcgacaagtt cgagtgcctg ctggggatga actggagcgg cggcaaccgc
    80761 ggcctggatc tacaggacgc ctccggcaac aagctgttca actacgatct ctcgtacgag
    80821 aacatcagcg cgatcagttt cggcctggac acccccgtgg acctgggctg ggcttacaat
    80881 gccacggcgg tcgtgcaggt cgtggccgag cagctcgccg gcaaccagtt gagcgtcacc
    80941 ctgacccgca acgacggaat gaccaccaac gtcgtgtccg ccgggctggc cgcgcccgtg
    81001 ggccgcatta acttctacaa cggcgggcac gatggcggga gtccgaactt cactctctat
    81061 gccaacgacc tgctcatcac ccgcggcggc ggcacgacgg acggcatccc gaatgcgtgg
    81121 tgggacaagt tcggcgtcgc ggcctcgaac cgcgtggcct cgctggatgt cgacgtggac
    81181 ggctcgccga acgacgacga gtacgcgggc gacacggacc cgaccgacgc gacgcgttcc
    81241 ttcgtcaacc gcgtcctcgc catccggggg aatacgctga tgaccctgca ggtcggcccc
    81301 aacacgacca acagccgcac ctacgacgtc tggtggagca cggacctgca ggccgatccg
    81361 caggtctgga cgccctacaa cctgaacgcg cccgcggccg tgggcggcgg ggcgctggac
    81421 atgctgatca ccaacgacac gccgcggaag ttcttccgca cgagcgtcaa catcccgtaa
    81481 gagatcggga agcggagcaa ggcgccccgg ctggccgggg cgctttgcct ttcccggcgg
    81541 agcgggccct tcgattcact attcactctt cgccattcgc tcctttatac tgcccccatg
    81601 aactggctct gccacgcggc cttcgccctg ggcgcctacc tgctgggttc cgtgccgttc
    81661 gcgtacctgt tcgcgcggct caacggcgtg gacatccgga aggtcggcag cggaaacgtc
    81721 ggcgcgacca acgtcttccg ggccgtgggc aaggggtggg ggatcgccac gttcgcggcg
    81781 gacgcgctca agggatttgt gccggcgtgg ctgttcccgc tgctggcccg gcgatggggg
    81841 gtggagtgcg gtccggaact gggattgctc tgcgggtgcg cggcgatcgc cgggcacagc
    81901 tggccggtct tcctgcggtt caagggcggc aagggcgtgg ccacgagcgc ggggatgctg
    81961 ctgggcgtgg cgcccgccgc ggtcgggatc gggctggccg tgtgggtcgc ggtattcgtg
    82021 atcgcccggt acgtttccgt ggcctcgatc gcggccgcgt tcgccgtgcc ggcggccgcc
    82081 tgggggctgt acgggcgggc cggcgccctg ctgcccgggg tgttgacggt gctgggcgcc
    82141 ctcgtgatct ggcggcaccg ttccaacctc gcgcgccttc gccgcggcga ggagcaccgc
    82201 tggggccgtc cccggtccgg cgcggcgcat ccgtaacaaa acacttcaca cgtgtaaagt
    82261 gtttcgttat cgccccggcc ggccgcgcgt ttccgctttc ccggacggga cgggcgttct
    82321 atgcttccac catgagttcg gaggccccta ccggccgcgt ggcccgtcgc ttctgggtat
    82381 tgtccgccgt catcgccatc ctcggcgtgg gcttgagcct gggggtgtgg cggtatcgcg
    82441 ccctgtcgga aacccgggcg ctcgaggcgg agttcttgca ggaagccggg acgctgcgcc
    82501 gggcgaccga gcgcgagatc cgcctgtttc tggacgtgct ggactccctc cgggttctgc
    82561 acacgctctc ggcccggatc agcgacgagg atttcgagga gttcgtgctc aagggcatgc
    82621 tccaccagca gcgcgtgctc ggcgggttcg gattcgtgcg gagcatcccg cacgaccagc
    82681 gcgcatcgct cgagcaggag ccggacgcct ccggacgccc ggccctgaag ctggtggaat
    82741 cggacgggcg cggcggttat gcgccggccg ccgcccgcga gcagtatttc gcgctgacct
    82801 atcagactca accggagcgg ctcggtcttc ccgcagggta tgacttcgct tcctgggcgc
    82861 ccgccgcgcg cgcgatcgcg ctcatggaaa cccgcgatgg gcccgtcctg ggaggcgtcg
    82921 cgacgggcgg gccggacgga acgccgcccg gatggtacgt gttttccccc atctactatg
    82981 tcgccctcga tgatgtcgtg ttccctccgc ccggctggct ggtcggtttc gtcacggccc
    83041 tgttcaagcc ctccgacatt ctggaccgcg tcctgctggg cgtgcccgct ctccggttcg
    83101 agatcgaact cgtggagcgt tccgcgccgg aaagcggcag ggcggcgctg cgctatgagc
    83161 atccggtccg cgtggccgac cgcgagtggc gctttcgctg ccgcccgggc ccggcctacc
    83221 cccggccccg gggcctgcgg gaatccgggc tgttcctggc gatcggcttg ggcctgacgg
    83281 ccctgatctc atggcagttg gcgacggtcg gccgccgcac ccgccagatc gaacgccttg
    83341 tgcacgtccg gaccgccgcc cttcgcgagg cccacgagcg gatcgcgcag gagatgaacg
    83401 agcggatgcg tctcgaggac gagatccatt ccacggccgc gcgcgagcag cagcgcgtcg
    83461 gccaggacct gcacgactcg ctgggccaga agctcacggg cgcccttttc ttgagtcgcg
    83521 gcctgctgcg gcaactggag caggccgggc ggaccgagca ggcgtcgggg cgacagctgc
    83581 acgatgcgtt gaaggacacc gttgccgagg tgcggcgcat ggcccgcggg ctggcgccgg
    83641 tttccatcgg cgaggacggt ctggcggacg ccctgcgccg gctggccgac gaatcccgcc
    83701 agacgttcgg cgtggcctgc gtcttccggc agtccggcgg ccatccgccg gcgggagccg
    83761 tggccgagca gctgtactac atcgcccgcg aggcggtgca caacgccgcg cgccacgggc
    83821 agccccagcg gatcatcctg gaactggcgg aagaggacgg gcgctggcgc cttgtggtgg
    83881 aggacgacgg gcggggcctg cccccggacg cgcagaagaa gggcgggctg gggctccgga
    83941 tcatgcgcca ccgcgcgaag acagtcgggg cgcaactgga gatcgcggcc ggcgcggggg
    84001 ggagcggcac gcgcgtgatc tgccgtcctt ccgccggctg acttcccgac agccccgccc
    84061 cgtggccggt ccgtaacgaa ccgattcacg gatgtgaata gtattgttac ggaggcgaag
    84121 cggcggcgcc ggacttctcc agcagctcgc gggcgtgctt gagcgtgacg ctcgtcaggt
    84181 cgcgcccgcc cagcatccgc gccacctcat cgacccgctc ccgggcgtcc agccggcgga
    84241 cgtccgtcac cgtccgcccg tcgcggacgg acttgctgac ggccaggtgc gccgccccgc
    84301 acgcggcgac ctggggcagg tgggtgatgc agagcacctg gtggcggcgc gccacctcgg
    84361 ccagcttgcg gccgaccgcg ctgcccatct cgccgccgac gttggcgtcg atctcgtcga
    84421 agaccatcac ggggatccgg tcatgggccg ccaggacggc cttggtggcg agcatgacgc
    84481 gggaaatctc gccgctggag gcgatcgccc gcagcaggcg catcggctcg cccgcattcg
    84541 gggcgaaacc aaaatccacg tcgtccatgc ccgacagccg gggctggccc tcgcgcacct
    84601 cgacggagaa cgcgccgtgg gaaaagccca gggcccgcag ctcgcgcgtg acggcctcgg
    84661 ccaggcgtcc ggcgtgctcg cgtcggcggg cccgcaaggc cgcgcccgcc cggtcgcgtt
    84721 tcgtccgggc ctgctcgatc tccttcccca gcgcggccaa ttgctcgccc cgcgtcttca
    84781 ggtcgtgcag gcggccgcgg tcgcgctcga ggacctggag gatctcggtc acggtcgggg
    84841 cgtatttccg cttgaggcgc tggtaggtgg cgaggcgctc gtcgagccag gcgagccggg
    84901 ccgggtcggc ctcgatgccc tccaggaatc gccgcagatc ggcggccagt tcctggaggc
    84961 ggccggccag ggcgccggtc tcctgccgcc agtcggccgc gcgcggcgcg aggcgggaaa
    85021 gtccgtccag ggccttctgc gccgccgcca gggcgtcgaa cgccgcgccc tcgccgtcct
    85081 gcagggcgtt gagcgccgcg ccgccgagct ccagcacctg ctgcgcgctg ccctgcagac
    85141 ggtgctcctc gatcacttta tcctcctcgc cgtcgacggg cgcggcctgc tcgatttccg
    85201 tgatccgata ggtcagcagg tcgatctgct ccgcggtggc gccgttctgc tcgatctgcc
    85261 ggacgcgttc ctcgaggccc tggagttgcg cgtggatctc ttcgtaggcg gcgcgcaggt
    85321 cccagcagtg cccgaaggcg tcgagcaggt cggtctggaa ttccgggctc aacagggact
    85381 ggtggtcgtg cggcccgtgc atgtcgacga ggagttcccc gactttcttg aggaccggga
    85441 gcgtcaccgg gctgtcgttg atcgcggcct gggacgcgcc ggacgcgcgg atgatgcggc
    85501 gcacgataag ctggccgtcc gcgcagggtt ccaggccgaa ggactcgagc gcctcgtcca
    85561 cggcggacgc atccgccagc cggaagcagg cctccgcccc gcaggcgtct tcccccgcgc
    85621 ggatcaggtc gtggtcggcg cgctcgccga gcaggaggtt gagggcgccg atgaggatgg
    85681 acttgcccgc gccggtttcg cccgtgatga cgttcaggcc gggctggaat tcgacccgga
    85741 cattctccac cagggccagg ttcttgacgc gcagtgtcgt cagcatggtc cctccttgcg
    85801 gccggctaca tgcccagctt gccgtggatg agcagggcgg tgaacacccc gagcagcatc
    85861 ccgaacagca cctccaggcg cgtatggccg aggaattcga ccagtttctg ctgcgaaata
    85921 tggtgttcgc ggaacagttc atccacgatc tggttcagca ggcgggcctg cagcccggcg
    85981 gcccggcgca cggtctgcgc gtcgatcatg acgatgaccg cgaaggtcaa cgcgagggcg
    86041 aagagcgtgc tgctgaaccc ggtgcgcagg ccgacggaga gggccacggc cgtcacggtc
    86101 gccgagtgcg agctgggcat cccgccgagc cgcagcatga aggccgggtc gaagcggccg
    86161 gtccggcgga tgtagagcag gagcttgatc agttgcgcca gcatccaggc cgcgaagccg
    86221 gaccagatgg tgatgtttat atgccagggg ccttcggcga tcatgggata cgtgtacctg
    86281 tttggcttcg cgatgtccaa ggcaaagtcc ccggccgcgg cggcaggggg cctatgcgcc
    86341 ggtcttccgc cgcgccgtgc cctccgtgta gaacggcagg gcggtcacgg cggccgggag
    86401 caggttgccg cggctttcca ccgccagtgc ggtcccgacc tccgccagcg gggccgccac
    86461 gtaggccagg gccacggcga cgccgaggct gggcgccagc gaaccgctgg tcacctcgcc
    86521 gacggcgcgg ccgtccttca tcacccggtc atgggcgcgg gcggcgcggc gggattcaag
    86581 ccgcaggcct gcgagtagcg cggtggtccc cccttcgatc tcgcgggcga cggcggcgcc
    86641 accgatgaag tccttggtca ggtcgatgta tttgccccgg gaggccccgg ccggcgtgcg
    86701 gtcctcggtc agctcatgcc cgtacagcgg gtagcccatc tccaggcgca gcgtgtcgcg
    86761 cgccccgagg cccgctggct tgacgccgac ggccagcaga cggtcccaga actcgcgcgc
    86821 gcgatcggcg ggaaaataca actcgtagcc ccattccccc gtgtagcccg tgcggctgac
    86881 gaggcaaggg acgccggcga gcggcgtctc cgcgaatccg aaatagggga ggtccggcat
    86941 cggggtctga aaggcctttt ccatggcctg ccgggaaagc gggccctgca catcgagctt
    87001 ggcggtcgct tccgatgtgt ccgcaaaacg cgttccggaa gagagatgcg atcgaatcca
    87061 ctcccggtcg cggtccaggg tccccgcgtt aacgacgagc atgaaacgat cgggcccgaa
    87121 gcggtagcag gtgagatcgt ccaggacgcc gccgtcgtcg cgcagcagga agccgtagcg
    87181 gcaccggccg gcggggatgg tggccacgga ctgggtcagg agccgctcga ggtcggcctg
    87241 cgccccgggg ccgctcaatg caaactcgcc catgtggcag gtgtcgaaca ccgtggcgcg
    87301 acggcgggtg tcttcgtgtt cctggaggat gccttcgtac tggatgggca tgtcccatcc
    87361 gccgaacggg gccatgcggg cgccgagttg ccggtgggcg tcatgcaagg gagtctgttt
    87421 catggatatc cttatacgcc aggaaccgcc ggcattccag cgcgctccga gtgctttgat
    87481 tatgccgcgg catgtgggta tactcataga agaaagaagc aggcatgaac agcgaccttt
    87541 cgcccattct gcgcggttgg gattatgacc cgggccgtgt gaacgcccgc tggatcatcg
    87601 gccgcgacgg ccggctgaag gtccagttgc gcctcgatct gggcgtgttg cagatggagc
    87661 cggaaggccg gcccgacggc cggcgcccgc ggggccatga ttccctgctg gactactacc
    87721 ggtcgctgca ggcggacatg ccggcgctgg atcccgctgc ctgtgcggag ttgcagcagg
    87781 aagcgatgca gtactactac cgctacctcg cgttccacgc gctccgtcac tttgacgggg
    87841 tgatccgcga cacggaacac aacctggagc tcctgaacct ggtcgagcac catgcggccg
    87901 acgacgacat ggcgtgggcg tttctccagt tctatcccta tatccgcatg atgaacgccc
    87961 gggcccgggc cgagaaaaac atgcgtgagc agcgggagac ggacgccgtg tccatcctgc
    88021 aggaggccgt gtccgacatc cgttccttct ggcggcggca gggggaggag ggggccccgc
    88081 ccggcagccc cgagatcgag gcgctcaccg acctgctggg ccgcatccgc cggcagggcc
    88141 cgcgaagccg tggcgaggaa ctgcgcgagg acctggcccg cgccatcgcc gaggaaaagt
    88201 acgaggatgc cgcccgtctg cgcgaccagc tccgggacat cggtgaaccc tagcgccccg
    88261 cggagcgctt attccttcat ggaccgggac ggccggcccc ggcgcccggg aaaaataaaa
    88321 acttgaaggg gaacggccga agcctttgat tcgtaacagg tgatagaccc atgaccatca
    88381 tcgaccccca tatccacatg tactcacgga cgacggacga ctacacggcc atgtaccgcc
    88441 agggcatccg cgcggccgtc gagccgtcct tctggctcgg ggcctcccgc cgctacgccg
    88501 ggacgttctt cgattacttc cagctcatcc tggatttcga gacggcgcgc gcccgccgct
    88561 tcggcatcga ccactacgcc tgcgtctcgg tcaaccccaa ggaggcggat aacctgcccc
    88621 tggcgcgcga ggtgctcgac ggcatgggcc cctacctcgg ccacgagcgc tgcgtcgcga
    88681 tcggcgaaat cggcttcaac cgcatcacgc ccggcgagga gaaggtcttc caactccagc
    88741 tcgagctggc caaggcccac ggcctgctgg tcctgatcca cacgccccac gacacccccg
    88801 aggtcagcaa gcggaagggc gtcgagcgga ccctcgatat cctgcgcgag ttcaactacg
    88861 acgacggccg gatcatcctc gaccacaaca ccgagaatac catggacctc gcgcgcaagc
    88921 ggccggtctg ggccgggctg acggtctatc cctattccaa gctcaacccc gagcgcgtgt
    88981 gcggtatcct ccggaaatgg ggcctggaac gcacgctggt caacagctcg gccgactggg
    89041 gcgtatccga tcccgtcagc ctgccgaagg tcgccggctt catgaagcag cagggcttca
    89101 cgccggaaga ggcgagccgg ctgctgttcg agaacccgat gaaattctac agccagcacc
    89161 cccgcttcaa accgcagctc gatttgccct tcatcgatcc ctccacctac cagcgatgac
    89221 cccccccgcg cccagcccgc gaaggaaccc gctccggccg cccgggaagc cggcggggaa
    89281 ttccgtcgtg aagctggttc tgctgggcct cggcctgctc ctgctggtgg cgctggccgc
    89341 ggacctgcgc cggatgccga agagaaagct gcccggattc accgatccgg ccgacgagtt
    89401 gcaggcggtc gtgctgggct tgaacggacc gcgctacgcc gatcccgaag gcctgttttc
    89461 catcgtcgcg cccgcgggat ggactgcttt caagccgccg gacagccgtc cgtacgacgt
    89521 cgtcttccgc ggcccgtccg gctcgaccat cagcgtcatg gccacgccgg tggagtacaa
    89581 cgacctgccc agcctggtcc gcgagatcga tcgcaacgag gaggaggccg gcatccgcac
    89641 gcagtcggag gcgttcttct tcctcggcgg cccggcggtg ctccgcaagg ccggcctggt
    89701 gaccagccgg gttttttccg tggattttgt ccgcgaccgc gtggcccacc atatcatctg
    89761 ttccgtgccg ccggagctgt tcgaccgata ctatcccgtg ctgatggacg tggtggagac
    89821 ctatcgtcct ggtgcgccga aagccgccgc ggttgcgaat gagtgagcgg cttccagccg
    89881 ccggccgcgc gcgcgcctgg cttcagctgc tgcgcgtgcc caacctgctg accgtgcccg
    89941 gcgacccgat cgccggattc ctgctcgccg cgggccggca ggccgcctcc ggcatggccg
    90001 gccggctctc ggccgcggtc gcggcgtccc tttgcttcta cgccggcggc ctgctgctga
    90061 acgatgtgct cgatttggaa accgaccgcc gggagcggcc cttccggccc ctgccgtccg
    90121 gcggagcaag cccgcgttgc gctttggcgg ccgccgtgtc cctgctgctg gccggcgtgg
    90181 ggttgagcgc ggcgctcggg ccgcgcgcgc gggacgtcgg cctggccctc gccgccgcag
    90241 tcgtgctcta caacggcggg ggaaaaaggg cgccgttctt cggatcggcg ctgatgggcg
    90301 tgtgccgcgc gttgagcctg ctgctgggtg cgacggcgtt ccccggcctg gccggctggc
    90361 cgccgctcgt ggtcgccggg gccggcgcgc tggggctgta catcctgtcg gtcacgaacc
    90421 tggcgcgccg ggaggcggag cccggcccgg tcggccgggc caagtcctgg ccggtgggcg
    90481 cgctgacgct gggcctcatg gtgttcgggg ccgtcaccgt gcgcctcggg ggcggcgggg
    90541 ccgggctctt cgtcgccccg tggttcctcg ccggttcggt ggccttcctg gcgtccgtcc
    90601 gcctggccgg tccgccggag cgggcacgga ggatacccgc cgccgtcggc gcgctcctgc
    90661 gcgcgctgct gccgctccag gccgcgctgg cctggggcgc cggggccggg cccgcgggcg
    90721 ggctcgccgg catcgtactg cttgcggtct ggccggtctc gcgcatactg gggcggcgtt
    90781 tctacatgag ttgaggccga ggatgaacgc gccgaaaaaa ctgctggtcg tggacgtggc
    90841 cgcgctgggc tgggatatcg tctcgcgcgc cggggcccat gcccgggtct tcgggttccg
    90901 gccgctcgag ccggtctttc ccgcggtgac ctgctccgcc caggcctcgt tccggacggt
    90961 cgcctggccg cgcgatcacg gcatggtcgg caacgggctg ttcttccgcg agctgcggcg
    91021 cgtgatgttg tgggagcagt ccgcgggcct cgtcgcgggc gcccgcctgt ggcgccgctt
    91081 ccgccaggcc ggccgccgcg tcggcatgat gttctggcag cagagcctcg gcgaggacgt
    91141 cgacctcgtg ctctcgccgc gaccgatcca caagcaccac ggcggcatga tccaggattg
    91201 ctacagccag ccggccgacc tctacgcgcg cctcatcggg gaaatcggcg cgccgttcaa
    91261 cctcatgcac tactggggcc cgctggcctc cgcgcggtcc agcgactgga tcgtcgaggc
    91321 cctggcccgc gtgatgaact cgccggacct cgcgcccgac ctcctgctcg gctacctgcc
    91381 gcacctcgat tacgacctcc agcggcacgg ccccgacagt ccggcggccg cccgcgccct
    91441 ggagcgcgcg ctcggatacc tcgcgcgcct ccgcgacgag gcggagaaaa cgggctacga
    91501 cttcctcttc ttcggcgact acgccatcga acccgtgacc ggtcctccgg tatttccaaa
    91561 ccgggcgctc cgggaagccg gcctgctggc cgcgcggccg gtccgcgggc ggctgtaccc
    91621 cgatttcttt tctagcggcg cgttcgcgct cgccgatcac caggtggccc acgtcttcat
    91681 cggcggcggc gcggacgacg gccgcgtcga ggcggttctg cgcgacctcg acggcgtcgc
    91741 ggaggtcatg gatcgcggcc ggcagtacga actcgggctc ggcccgaccc ggacaggcga
    91801 gttcgcgctg ctggcggaga aggggcggtg gtttgcctat ccgtggtgga acgagccgcg
    91861 cgaggccccc gactacgccg gccacgtgga catccataac aagccgggct acgacccgtg
    91921 cgaactcttt ttcggctggc cgcccggccg cgtgagctcg gatcccgcgc gcattcgcgg
    91981 cacgcacggc cgccccggtg tgccggcggc gtgggccgcc tccttcccgg tcgagccctt
    92041 gccggggacg ttgctcgacc tggcccgcgc cgtggagtgg cgcctggacc ggtccctcga
    92101 cgcatgagcg cccgcctcca gaaggacgcg gtgctgcgcc agtccttcgc ggtgccggtc
    92161 gagttccccg tgatcttcac gcgcgggttg ttcgatcccg cgaatcccgt cctgctcaag
    92221 gccgtgcggc ggatgggcga gcggcgccgc caccgcgtcg cggtgttcgt ggatgccggc
    92281 gtgtggcgcg cgcacccggc cctggcccgc cgcatccggg cctatttcgc cgcgcacacc
    92341 ggcgcgctgg agctggtcgc gcctccgcag cgcgtgccgg gcggcgagaa ggccaagcgc
    92401 gacgccgccc tcgtgggccg cctgctggcc cgtttccagg cctggcggct gtgccgtcac
    92461 tccttcgtcg tggccgtcgg aggcggggcc gtgctcgatg cggtgggcct tgcggccgcc
    92521 ctgtttcacc gcgggctgcg cctgatccgc gtgccgacga ccgtgctggc ccagaacgac
    92581 gtcggggtcg gcgtgaagaa cgccgtgaat tggcggggcg tcaagaacct ggtcggcacc
    92641 ttcagcccgc cgttcgccgt gctcaacgat tcggatttcc tggcggcgct gccggaccgc
    92701 gactggatcg gggggatcgc cgaggccttc aaggtggccc tgatccgcga cgcgcgcttc
    92761 ttccgctggc tctcggcgcg cgcctcgcaa ttgcgcgccc gcgaccgggc cgccatggag
    92821 g
//
LOCUS       DAOOTK010000003        68913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_322_length_68913_cov_7.088922, whole genome shotgun sequence.
ACCESSION   DAOOTK010000003 DAOOTK010000000
VERSION     DAOOTK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 68913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68913
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_322_length_68913_cov_7.088922"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PK634_00840"
     CDS             <1..960
                     /locus_tag="PK634_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96312.1"
                     /translation="AITGRPAATPVDIISPKGDGVINVQPNAEMPNIGEAGDIVVYLE
                     PDAGDMREAKRQRCWHNDILATRHGDLFLMVTAATRKTIEPVLCVENKLLRTDKRGQF
                     HLFRPHAPTFPLNDIYQQQVDGWTQIYTLANLVNDDTDMESDPADFTDVEALRRLAIS
                     LFHVNSEADTQSLLMVSCAQWVWSIMLLAFCIPLNNHTMTELGVEDIWNNRFRDIPRC
                     DEKFQISSVLPMQPFSKDEAVVALAANYAGMHSLLWDQSLVSKILMLVKLDPQIASTH
                     NALPDRIIWPTFPLWEHRHKRPSTLMEFEYIATVFPDEVCVGA"
     gene            complement(1874..2380)
                     /locus_tag="PK634_00845"
     CDS             complement(1874..2380)
                     /locus_tag="PK634_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOW96313.1"
                     /translation="MMNIKYMEYALDQARTALANNNVPIGAVVVHEGCVIGCGYNRVM
                     GGDRFGHAEMMALRMAHDAIAALRISSRRRVELFTTLEPCMMCLGAVMQVNIGKVIYA
                     LKAPTDGATHMAGPQVVHSAQIMKFYTPPIVEKGGGESESRQLLKKFYTEHSAHYLGK
                     WALLLAEQ"
     gene            complement(2481..3941)
                     /locus_tag="PK634_00850"
     CDS             complement(2481..3941)
                     /locus_tag="PK634_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96314.1"
                     /translation="MRAILLEEPELEFGAGTHVDIRFGLMESGPLDRDTDLAPRPLKV
                     GVVGTKKTCDAACEWLQKLRGEIPAQQTHLSNLHPPFPGIDASSCFGTELQIHDRWIS
                     PVSMREVDSMISSCTPAELAERSVDLFLSHADDLVDAGGPSVLIVAPPPELFAALESD
                     PRPPIDPDDQELDESTEATPSAATPCFHDILKAKGMRLAVPIQMVRPRTYGMTAGNKK
                     GHKGRKTLQDEATRAWNFAVALYYKAGGTLWRIPRQTSDLTACFVGVSFFKIPNSNDA
                     ASSVAHVFNERGEGIIIKGGDAAWDKSEHQYHLSRNDSFDILSSALTLYRKEHHTVPA
                     RVILHKTSPFSDAEQDGFKGASERERIAYTDLIHVRRSYSRFFRTDIYPPFRGTLVEL
                     EDARGLLYLRGSVSFFQAYPGLYVPRPFEFCLCQSENGVEEVGEEMMALSKLNWNNTQ
                     FDGGEPITIRAARRVGDILKWIPQDVSPRNGFRFFM"
     gene            complement(3938..5287)
                     /locus_tag="PK634_00855"
     CDS             complement(3938..5287)
                     /locus_tag="PK634_00855"
                     /inference="COORDINATES: protein motif:HMM:NF025638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HOW96315.1"
                     /translation="MTKNLHRKAVRGQEGVNLVERVVLDMKSRWTPSGPNEVGIDGFI
                     ELFDSTTGKPTGKNIAVQSKALAALCRKGTDTIEYWCDRRDIEYWLRQNLPVILVVSN
                     PDTPEAYWACIHDRFPDYSRHVSPKIQFDVKADAFSASAWAALRGLRTPKIGGKTFIE
                     ACQPGTERAVSNLFPLRRFSGTIYHGMTDMRYPIDVWRSLREAADFNDGAWVLHDKGV
                     FSFADLKSPEWNAICDKGTVEPFDTAEWADSSNADSQRLFVQLLNRALKSQLSPRVRF
                     WPKQDCFAFVGGPWTGRVVFPYQSAEKKSHVTVVEEFAHDTADGRTFVHYRHLAFRGR
                     FRKLQGKWFLEVTPTYRFTRDGNWLYRFHEDQLKGIKRIEGNRAVLSQVLVWAEFLCE
                     TDDMWTSVPRHLEFVRPVEIELPAILRDSAWGKPPQPPSEDSDDSIMELPFGDEVMS"
     gene            complement(5454..6968)
                     /locus_tag="PK634_00860"
     CDS             complement(5454..6968)
                     /locus_tag="PK634_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96316.1"
                     /translation="MLTKREQSNLETHVLGIFQTVVLPEITPGGAVLTREADGRVIED
                     YVATHHLVELCVEVDATLFKQIIHTACQWFAKTHGNPHNPFMVTTLARAGAFNDTTRD
                     DIAKAILAKQKPSGFFELYSGFLDGGNIFSTLWAIRILQLLGRHEATSPPIRKALNAI
                     EGQWEDVHRTSFKGFFFELRHVPSHGKRPSAAATKLLTEIIKAQADNGLWDGEALYTA
                     YVLGNLTKTPWEPTSTQQFAEDHALKALFSLRDSPQSLPSVFLKARKKYHESTYLQLC
                     FRSLISSIRYLRMRCGKDMSHAIASVVMGSFPTIYHTAQRLGAELKRMDAQYGAIQAQ
                     FSHLDPSASVLLKESPYERNVFVMMPFRKDRDERYERIESIIRQELKKHGFTAWLASD
                     KTVAPQLWDNVASFLLACKYGIAVFTRIEKESTIQEEFNPNVSLELGFCLSRGRSVLI
                     LKDSFLPRLQTDLIGYLYKQFDLNQVTRQLPRIVRAWIRDILKAEECAKKQPTK"
     gene            complement(7662..8153)
                     /locus_tag="PK634_00865"
     CDS             complement(7662..8153)
                     /locus_tag="PK634_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96317.1"
                     /translation="MMTCRIESWLRQHFPPEISDDVKEHFDIIPKVAAFDGISFGPRL
                     NFLVGESGSGRTRLLERLKTSAGIIELPAPPPVSFTAMSHGQVVFTVGMLLVKSTTQK
                     TALLVDDILFMLDDRLAEQFLVGVVASNKQAILTVTLGEMERAERLMRKAGVSGYGIV
                     WQK"
     gene            9636..10151
                     /locus_tag="PK634_00870"
     CDS             9636..10151
                     /locus_tag="PK634_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96318.1"
                     /translation="MAILPEVSGNANWKTIPKGTFLAICEDIKDEFGVHRKKYNSEEM
                     EVIDQTQFRFVFKDPKSGEIFRITSRKMRISSHEKSSLYQALHAWCGEPPRAGWDFGE
                     LKGCGAMITIAHMPSTLHPGQPFATIHNIGPVPEDWNDKLEALKKAVADPAAKNGVKE
                     AAGVNMPGEGW"
     gene            10184..14665
                     /locus_tag="PK634_00875"
     CDS             10184..14665
                     /locus_tag="PK634_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOW96319.1"
                     /translation="MSEVFTIPDEIPSHKLAAARLYRDLGWAVHPLHGPSEGGPSERG
                     KKPRLRDWKCWSVEKVTDEFLSTYFSNGTTSNLGVVVRRPWVFIDLDSKKDQGASVRA
                     WLDDQPAQAHLKNVPRYSTGNGERLVFVCYDLPQFKKSNGKRYEKALISHINEKLTIE
                     LYYDGLNIVLPPSIHPNGSAYEWRTKGEVPTVTWSQLQQWFGFRLPTDEGETTDSHTV
                     RRQNKAAWWAKYQGDLRTLDIAELFHDAGMLGDLVTPDENKYSALCPWRTEHGDKAEA
                     ADDSSTVIFAPPDSMPGFSCLHAHCAERHLAEVLDWFELAEPGCVDLHCKRMRVFQPG
                     QTAQDGRPRLILPGVGRSASDFAKDAGAAIATSTSVRRLFLRNGLVVEISQIQFAKGQ
                     LCLGFAVVKPARLITLMESYAEVGFLTKDDLGNDVFNARSMDKNTAEVVLAAQQFREQ
                     LPVVERILDVPIPILTPEGIICYPSTGYDPRFMTYLAEDAPKIERMPVADALALLREL
                     YFEFCFRTAQDITHAIAALITPFCRGLFGRWTARTPTWFHGANRARAGKDCEANLASI
                     LYEGRTNEDAPLTADVAETRKKITSALIAGRRRIHFANCKGHINNSAFESIGTAEVWT
                     DRVLGGNTTISLPNEMEISLSANTGLTYTPDLDARCRPIMLFYSEEDPNARTFQRPDL
                     HGWLRANRGRVLSAIAALVDHWDMQGRPSGPSPFTSYPLWAKVVGGIMTSAGLGDPCL
                     PVPNDGTQIAGDQQTGDMKTLFALAHEQFNGEWVNKGRIMELLEGEGTELFGWLDLES
                     RGGRTSFALLLNKHVGRVLGGIRLERNASAKKSQQHTYRFVKDAGGSTVNARAAMFDT
                     VFNSAKVGESPSGGGHVGHLGHLPYTPYIKTNSSNNIEMKNKEREIYKSIEQVPNVPK
                     VPASSTIPCFDLVTSSDALKGIADQITAASEPVALDIETYGAKGGLDPWQGDIRLLSL
                     AIPGVSPWVLDLKAIGYDLGPLGAVLPAKPVLGHNIKFDALWLRVKCGLVLRNVLDTM
                     TASRLLTNGTKEPNDLGAIISRHLQIDLPKDQGRSDWGAMALSEEQLRYAADDVHHLH
                     ALHGKLQGELEKAGLLTVYETEMDLLPVVVDIEARGFAIDKVRLAVLSKKAAEERDTA
                     ATVLRQTFNAPALNPASPAQLKTVLAGVGITLESTGEEALQASGDDRFVPHILAYRNA
                     QKRTEQTEKLLQKIGPDGRIHGRFEPTGTETGRFSSKDPNLQNIARGELRNCFVASPG
                     CSLVVADYSQIELRAAAAIANEPKMIEAYRQGADLHKQTASLVLGISPDAVSKEDRQL
                     AKAVNFGLLYGMYAATLVKYAKTSYGVTLTDQQAVQMRERFFNAYRGLRRWHDKAGLE
                     AGGAKEVRTLLGRRRMLPAGFDKRWARFSTLLNTPVQGSCADGMKKAILLVASRLPYG
                     AGIVSTVHDELIVETPSEQAEAVNTIVHDAMVEAMAAIFPQVPIVVDCKACQTWGEK"
     gene            14927..15535
                     /locus_tag="PK634_00880"
     CDS             14927..15535
                     /locus_tag="PK634_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96320.1"
                     /translation="MEKYRSLVQIQFAGGLGSDLFWHPSFLRKSDAAAPLYQSRCAKQ
                     RIIWAEKEYRRTHPKAADIFVQSEASITRKYLKQLKSRGLYGVLAAHLFRAQKSSARA
                     KLYRHGDYKQYAYDRKGQILQKLCEFLPSLPEIKWGWGQDDKGYLADVLYIEIPTGQV
                     SFHSESRYAGPDYPGAWDGQRASEHRIMAFCDSVMGALIEHI"
     gene            15777..16388
                     /locus_tag="PK634_00885"
     CDS             15777..16388
                     /locus_tag="PK634_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HOW96321.1"
                     /translation="MKKSKPAGDNPANSDADNKVCRNKTAEHARQVLTSTGNNSWATP
                     AVWFEYLNLEFKFDLDPCCTHKTAKCKKHYTPAEDGLAQSWAGERVFMNPPYSAELPK
                     WLRKAYIECRDNAALVVALVPARVDTDWWHQYAAKASEIRFPIGRLTFKGAEYPAPFP
                     VAIVVFRPRISNPYSEPMWDMPDDTTQDEYYAWLDQQEQGGAS"
     gene            16385..17527
                     /locus_tag="PK634_00890"
     CDS             16385..17527
                     /locus_tag="PK634_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOW96322.1"
                     /translation="MKKHLSLFRYPGGKYRLIGSIREKLDPLLRDAHLYVEPFAGGFS
                     VGLSVAQDYPDIEIWLNDLDQDVSAFWHVLASGSDRECQRLLNLIIQAPSVELLEQLR
                     ATPPKDQVERAYRAIFFNHCTFSGMANSGPLGGYGQDGKCHIGDRFNCRRIIAECIEA
                     RKLLRGRTKVFCQDGIQLIREAPVDAVLFVDPPYVKAGKSVYREYFQTEQHKALAEAL
                     RGRTRWVATYDMAPEIKDHYDWAILEELPVNYSIKSFNTGLTRSTEYLITPPAPVPET
                     ASEVVYSSSSSSESLDETAHAGKAKRQEEKDLGRDHHPAVSLAKSTVNFADYEYLTTK
                     EAAMYLRRSPSWLLHNKEIPYLTGSPNTYKKSDLDAWFNKTKSGEI"
     gene            17530..18606
                     /locus_tag="PK634_00895"
     CDS             17530..18606
                     /locus_tag="PK634_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOW96323.1"
                     /translation="MSVWQRKDHLDKWSHPVWYYSFWHRGQRYRKGGFLTKGRASAAE
                     AKRKHDVIIEGKVFRGKGNKSLAEIFSEYLEYRACDCAASTIAGEKTRRPPLLGFFNK
                     TPMTSIAVSDVQKYVEDRKRKGMANRTVNLELITLRNLFKFAMQREYAFQNPAKEVKN
                     LKEVLRDRPVPNPEQMRALLEAGKAEGSQVYCWMALMAYTGMRPSESFHLSWDDIDFK
                     RMQILVRPKQEHDLKTGRFRAVNILPELLPILLAWQTEWEQVFADGKPHNWVFFHPRR
                     PAFRAHGYPKHFKRAVKNAGLKGFTPYTFRHYFCSRAIESGSNIEAVKIVVGHTPSST
                     VLQKHYAHLCQPFIDAELAKVKIV"
     gene            complement(19016..19091)
                     /locus_tag="PK634_00900"
     tRNA            complement(19016..19091)
                     /locus_tag="PK634_00900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19056..19058),aa:Phe,seq:gaa)
     gene            19160..19564
                     /locus_tag="PK634_00905"
     CDS             19160..19564
                     /locus_tag="PK634_00905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOW96324.1"
                     /translation="MNSCPFCAVAPERITAEDGPCLALSDRYPVSRGHQLIVPRRHVA
                     SFRELTDEEWQAVLRLARRLAEQLQESDPAIQGFNLGINDGAAAGQSVFHVHAHLIPR
                     RSGDVRRPRGGVRGVILGKQDYPGDSAPAPTG"
     gene            complement(19682..21256)
                     /locus_tag="PK634_00910"
     CDS             complement(19682..21256)
                     /locus_tag="PK634_00910"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOW96325.1"
                     /translation="MRFDVLIRGGQVLDGTGTPARRADIGLLGDRIEAVEDLSLAEAG
                     VVVDAAGRCVCPGFIDVHSHSDAYLLIEPAAPSKVFQGVTTEVVGNCGASAAPRLGRY
                     LMPSDWRAMPYPGSWRTVAEYRALLEQVKPAVNVVLLIGHNALRASVMGYEERRSTAD
                     ELAEMKALLARCMDEGGAGWSTGLIYSPGMFAAPSEIVELARVVAGRKGIYTSHMRSE
                     GGRLLEALDETIGVGRETGVRVQVSHLKTSGRANWGLREAALDRVRAARAEGLEVAAD
                     RYPYTASCTDLDVILPDWAAGGGREEVLTRLRDPATRARIREEIAARRDAAYWENLVI
                     GSVRHPGNAGFKGRPIVEVARRLGLEPVDAALHLMDTDELHTSGIFFGMSEDNMWRIL
                     AEPYVMIGSDASLRAPSGPLSRDHPHPRAYGAFPRFLRASLDGRTVSPAEAVRKMTSL
                     PARQFGLKDRGLIEPGMKADIVVFDAGGVGDAATYEEPHRLARGIETLMVNGQAVLSG
                     GKPTNVRPGRFLSSRR"
     gene            complement(21253..23289)
                     /locus_tag="PK634_00915"
     CDS             complement(21253..23289)
                     /locus_tag="PK634_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96326.1"
                     /translation="MATEHTEPIAPPGGPGITSGPFSKRRGFWLPALLLMLAGYLVYS
                     NSFHGTLILDDLNSIIRNEGIRRLWPLSAALHPPKGPISFWTRPIVNVSLAWDYAKSG
                     ENLAGYRRTNLTLHLLAGWALYLLLIRLLRGGERRAAGSPWTDPERLPGSLRAVFLDG
                     AQLAAFVATLLWLVHPLNTAAMNYLSQRAELLMTLFLFVTLYALCRSADSVAGHDVAW
                     SAVAAFGCLLGMGSKETMLVAPILALLLDRAFLAASWRDVLRLRGWLHGMLWLTALWP
                     VSRHLSYSPHVTPELLAPGMRWHYLLTQCWGVARMIRLAFWPQPLVFFYGMDLIDNPL
                     AVLPQALLLAALAGATLWAVARHPRAGFAGAFFFGVLAPSSTFVPILGQPVAEHRMYA
                     PLAALVALVSAGAFLAAVRTRRKEDLSLYLAAGLILAAGFGWAAHRRNADYRDPLVLW
                     ADTVRKVPGSPEPHNDYGNALREAGRPAEAAEQYRQALRLKPDYMNAHNNLGTLLAGL
                     GRYEESEEHLREALRLKPDSPQLEVNLGMVCASLGRNEEARAHFESALARMPGNPQAL
                     AQWAMTLADLGRAGEALERLQAALAARPDDPELNNALGVTLCRLGRPAEAADPLRKVV
                     AAFPGKGRARFNLGRALFDAGRLEEAEAEFLRVGSPEARRYIEKIEQRRAGGGP"
     gene            complement(23279..25102)
                     /locus_tag="PK634_00920"
     CDS             complement(23279..25102)
                     /locus_tag="PK634_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96327.1"
                     /translation="MAAGLLAYGNSFHRVLHLDDLKSIRDNEGIRKLWPPSEVLNPPT
                     GTISFYTRPILNATFALDYAVSGTDWSGYRRTNLLLQVLSGCALYFLLARTLRLRPVG
                     PEKGSGEGACPLPAGWRGFFSDGADRLAWIIALLWLVHPLNTAAVNYLSQRGEILVAL
                     CLWTLLYCVGRAADGAGWRARWWQAAATACCLLGMGSKEHMLAAPLLALLYDRLFLAD
                     SWRALWRQRGLMHATLWLTAWWPIHRQCMYSPHITEAALAEETRWRYLLTQCRGLVRM
                     IRLALWPSPLVFYYGRDLATDLSAVWLQALLLGMLAAATAWALVRRPRAGFAGAFSFL
                     VLAPSSTLVPIIGQPVAEHRMYAPLAALLALGVGALQVVLSRMVGPRRAAGTLLGLAL
                     IGAIGLGAVTRCRNADYRDPLILWRDTVRKVPLVPEAHYDYANALREAGRLDEAVAEY
                     REAVQLSPDYVDALVNEGALLAMGHRAAEAIARLEQALQLKPDNPLARQNLASAYLEA
                     GEFAKSAEQLDQVLKAVPFEPLVTVQWAQTMKEMGRGEEALGRLRDTLKVHPGHPALL
                     HAEAEIRESLNRFRGPMSGGPGITPGVGLPPEKRPQEGHGY"
     gene            complement(25161..25733)
                     /locus_tag="PK634_00925"
     CDS             complement(25161..25733)
                     /locus_tag="PK634_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96328.1"
                     /translation="MNQQEWNIKEPCAECKACHRAFADKEPIWSRLTFGAEGYERQDC
                     CAVCWPGAQKGDSVSAWKSVYHMPAPPPPEPIKRETAESLLRQFMETEDPSRRSAIFI
                     LAVMLERRRLLVERDVKTREDGLKVRFYEHRKTGEVFVVPDPGLRLDELEQVQQEVIA
                     LLGGPVPEKASDKKEKPSFDESRGDGPLGS"
     gene            complement(25898..28213)
                     /locus_tag="PK634_00930"
     CDS             complement(25898..28213)
                     /locus_tag="PK634_00930"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96329.1"
                     /translation="MCCGCLAALAAGAQTNAPPSDRMEFADGLYVRGMYDLAVKEYLL
                     VIRDEPPGVAIDAALFRAGECHRQLGRREMAERYYKRILTDFPQSPHRFLAEFRRAEL
                     FVTAGQYLDGANLLRALLDAGPPDDVAASAAYFLGTSLDKLGQAGEAEQHLQTVIDRH
                     PKSPFYGYACQALADLGQRTGKPAAEVRDLYRRAAEQPATPRAGAEALFQWAEHAFKA
                     GDFEESAKAYEQLLEKYPGDRRVPEARLQIAWAFLQSGKNAAALKLAEERLAAGEEAG
                     REDWLYIKANALRQLQRTDLALAAYDELMMQFPSGPRRPAAAYEKALIAFRRGQHEKV
                     VEQLAGLAPSAAVEEDRGWLLAESYAALNRSAEALEQFQALAEKFPESDRAAQALYRQ
                     AELQQAAEDFAAAAEACRKIAEKYSRDPLVPDALFTAGYCHARLDRHDEALRDWTALL
                     EKHPDYRQSDVALYQVAVAQIRLGKESDARRTLERLLKDYSKTERAVDALVALSILHE
                     QAGKLDKAEEAVRDALARKPAEAELPRLKYRLATLLQKQGREDESAALLQALLDTPIR
                     RDMPPAMLEWLADLQLRHEAPADALKAAQALADQAGTPEWKQIGWYWVGRSREAAGEQ
                     DRALEAYARALDQEARTLEAAESALRLGDLRLATGDAALAETAYQAAAERATEPEAMS
                     LRARSYFGLGRAAAARENWEDAARFYMSVAVLYDDPELAPRSLHEAAAAFDRLGRGED
                     RQKAVDELTARYPDSEWAKELAGKQAPPTIP"
     gene            complement(28244..28672)
                     /locus_tag="PK634_00935"
     CDS             complement(28244..28672)
                     /locus_tag="PK634_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOW96330.1"
                     /translation="MNFRPAIRQEPGAFPLAPMMDVIFLLLSFFITTQIFAQWETEID
                     ITLPTAETGQTPQRLPGEIIVNIRPDGGAMVNNQALDDAALGAMLKRLASLFPGQPVV
                     LRADKAVAYEHVVRVLDLCRKADIWNISFATSLPESPAAP"
     gene            complement(28669..29400)
                     /locus_tag="PK634_00940"
     CDS             complement(28669..29400)
                     /locus_tag="PK634_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOW96331.1"
                     /translation="MWKRAGGWAVGLAVAAGPAWAQAGEAAPKGSGQGMNLVEIFKAG
                     GWLMYPIAALSVLAVAFILYLLIVLRAEAVAPRRWLQKVFELLRQGHADEARGLCDGR
                     PSPLAAVTAAALDYVAEAKPVQPDMLKEIMVGEGIRQATRIQNQVQYLMDIAVIAPMI
                     GLLGTVVGMLQAFNAIALDLAKAKPMLLAAGVSLALITTVAGLVVAIPAMIFYAYFRN
                     RSSAVVARLEMASTDLLTHLLGRAS"
     gene            complement(29484..30308)
                     /locus_tag="PK634_00945"
     CDS             complement(29484..30308)
                     /locus_tag="PK634_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOW96332.1"
                     /translation="MKRGMLALGCVLALAGGAAAAEPKKVSIKGSNTFGEELGPALIA
                     EFRKSHPDVEIALESRGSGSGFAALFAGECDIASSSRSVNADERRLVAKRKLDLRNYT
                     IGYYGVAVIVHADSPLKGLTARQVRSLFTGRTKNWKEIGGPDAPVNLYIRDPESGTHL
                     GFRELAMADKPYDKDAKMFDHYAAIARAVAADPAGVGYVDMAVAKQDGIRVVSINKIR
                     PSVETINDGTYPYARQLHLYTIRSRESKAAKAFIDYIRSRAGQRKLTELGYVRRFE"
     gene            complement(30330..31715)
                     /locus_tag="PK634_00950"
     CDS             complement(30330..31715)
                     /locus_tag="PK634_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW96333.1"
                     /translation="MDNSTGQESGGRLQHPEGFRARRGLNWVSLGLLYASYYMCRYNF
                     RFATPGMVEEFSFTKTQIGDMLAIWSLAYGTGQLINGLLCDRIGGKRSMLIGAVGTIL
                     LNLFFGFGSFVGSFATFSLIWLINGYMQSFGAPGMVKINAAWFARRERGTFAGIFGFM
                     IQLGQVAISQLAPVILLGFTFGVLVVGEGEWRWLFRIPPLFTAACAVFMALAAKPTPD
                     EAGYPGVIKDELDDSAGVTVSLKESFKTIFTHPLVWYYAMAYACTGAVRTSSDQLAIL
                     YFQDELGFDMKTEIPNAARLTLSLMPMVAVLGSFLSGWVSDKVFKGHRSPVAMGLYFL
                     EAIVISVAALVLLSGAVGPTPGGIFLGCLFLVLIAFTANSTHSVVGSAAPMDIGGKKM
                     AGFAAGVIDSFQYYGGAISLAITGRVLDATKEQYGYTFWYAIMAGFGVLGGIAMLLVT
                     RKQRRRHIVAG"
     gene            complement(31782..32882)
                     /locus_tag="PK634_00955"
     CDS             complement(31782..32882)
                     /locus_tag="PK634_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96334.1"
                     /translation="MKPGLAYYISAHGYGHGVRSSDILRAFIRGYPGIPVVIVSDLPG
                     PFLRNRLAGAPADIRPGSFDVGMVQLDSVRVDVPATLDRVRALCGRREELVGREARFL
                     REREVGLVVADIPAMPLEAARRAGIPAVAVGNFGWNWIYEEFIPRDPRWAEAAAAFRE
                     GYARADVLLRLPFHEPMAAFRRIEDIPLVAAPGRARREELAALTGARPDRTWVLLSFT
                     TLEWDGAALDRVERLGAYEFFTVRPLEWSRRNVHAVDRERMPFSDVLASADLVVSKPG
                     FGLVSECVVNRKPLVYVDRADFREHPILEAGVKRFLKHRLLPADRLYAGDLEEALDAI
                     VREPEPADELPGGGAEIAARRLAERLDPKNGL"
     gene            33005..33847
                     /gene="ppk2"
                     /locus_tag="PK634_00960"
     CDS             33005..33847
                     /gene="ppk2"
                     /locus_tag="PK634_00960"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOW96335.1"
                     /translation="MSKEKKGGQDRGGDEEKDDAPHGKLKRKAYEKELGKLQVELVKL
                     QEWIKYKGLRVVVLFEGRDAAGKGGAIKRITESLNPRICRVVALGTPTEREKTQWYFQ
                     RYVAHLPAAGEMVLFDRSWYNRAGVERVMGFCTETEYEEFLRSCPEFERMLIRSGLIL
                     IKYWFSVSDEEQERRFQDRIHDWTKRWKLSPMDLQSRARWVDYSKAKDEMFKYTDIKQ
                     APWYVVEADDKRRARLNCIHHLLSIIPYKDLTPVKLKLPRRQPDTGYERPPITDQTFV
                     PEVY"
     gene            complement(33855..34628)
                     /locus_tag="PK634_00965"
     CDS             complement(33855..34628)
                     /locus_tag="PK634_00965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOW96336.1"
                     /translation="MFPLRDDNPTLRASVATMAIVAVNALVWIFVQRLGMDPGLSESV
                     WRFGLIPGELLGRVRPGTEVPAGPGLVAVLDGAPNWWSVLTSMFMHGGWMHIIGNMWF
                     LLVFGDNVEDAMGPVRFVVFYLLCGLAAAAAQMLSGPSSLVPMVGASGAIGGVMGAYL
                     VLYPHAPVHLLVWIGFYANRVVVPAFFMLGYWFLLQLVAGAFDTGHGGVAFWAHVGGF
                     AAGALLVRPFCNPARLAAFRDRRARLWSARNPGGYGRWA"
     gene            34881..35477
                     /locus_tag="PK634_00970"
     CDS             34881..35477
                     /locus_tag="PK634_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOW96337.1"
                     /translation="MEPTDETLMQAYGQGDETALAQLVRRHSGPLLGYLTRMSSNPHQ
                     AEDLFQETFVRVHRHAQNFRPDGRFKPWLYTIATRLAVDELRRRKRTPVLLSMDEQSE
                     EHHSLADRIADPAAGPADCAAAADLRARVRGAVERLSPAQRAILSLTYFEGFTYPEAA
                     RALGRSLGTIKKQMSRALRSLARALPDLAPGHAAGGAS"
     gene            35474..36553
                     /locus_tag="PK634_00975"
     CDS             35474..36553
                     /locus_tag="PK634_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOW96338.1"
                     /translation="MNHHLKEQQILDYLFGLAEEAESARTEQHLAGCAECRKHADLLR
                     RRFAALESLRETEAASERLIADTLRRVRVDHPVSESRFPSFAWIAAAAAALLVAVFIV
                     APRIGREPAMELTQTELSPAPAGGGVAGIPPASVAEPAAAPPPAAVPPPVEPPTGMLL
                     ADSAPMIAGEVADRDKALAEADEILSDAAGQPAAPMLAAARAFAPERSAGAGISEPPA
                     PKTDATTVAKKTVHRPRKPRPADAKPYTARLPHGWSFAPTNEARVMLIPLKSKADGLM
                     PPPAEGFRTRHWQVRVVNDATNAQLVSVTRTFDQPGWDLQLLNDEGRLARRSDLEAVI
                     EADVPAGGVRAVHCTISEPLKENQP"
     gene            36550..38985
                     /locus_tag="PK634_00980"
     CDS             36550..38985
                     /locus_tag="PK634_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOW96339.1"
                     /translation="MKSLLCSGLVMCLCAASAAAKPEAPTQGALSTVIKDDNGKETII
                     DLPLKHTEVEAEISGYVARVKVKQTFTNPCDQPIEAVYVFPLPQNAAVNDMQIRVGDR
                     VIRGLIKKHEEARQIYEKAKQEGKTAALLEQERPNIFSQSVANLMPGSDIVVEITYDD
                     LLPYEQGRYEFAFPMVVGPRYIPGKPTGKQAGGWAEDTDQVPDASRITPPVLKPGERN
                     GHDIMVTVKLDAGVPVQALECPSHEIVTEPGSAPFDGPATVRLAPRDTVPNKDFVLRY
                     TVAGDAPQTGFLAHRDENGGAFMLMFQPPVQPQLDRIAPKEMVFVLDCSGSMSGEPIS
                     LVKKAVRHALQNLNPDDTFQIIRFSDASSSFASEPVKATPANVRRGLSYVNGLDGEGG
                     TEMLEGIKAALDFPNRERRLRIVCFMTDGYIGNEKEILAAIRTRLGDDTRLFSFGVGS
                     SVNRYLLERMAEVGRGEVKYVLLNEKPEDTVKKFYERIRSPVLTHIRVDWAGLEVEDL
                     YPARIPDLFAGQPVFVLGRYGRPGAATIRVHGKISGQPVTYELPVELPDAADGNAAVA
                     SLWARSRIRELSTELYGGEDKDVVEEITALALKHRLMSEYTSFVAVEEKIVNEDGHPV
                     TIQVPVEMPEGVSYEGVFGGEPAPMQVGGRGGGLFFGAAMARKMVAPAAVAPRSLEAD
                     EDSAGVPEPTEPMKPFSIELRTPAQGGEEVLRITEGGEIWLERGSTERMLLGRLTRRQ
                     ARELWALLAKALKAPTEKTPWTRAAELHIRLRGDDGQPQPETRVETSQATDFPPSPWK
                     DLLDYIEVLRAGF"
     gene            complement(39022..41094)
                     /locus_tag="PK634_00985"
     CDS             complement(39022..41094)
                     /locus_tag="PK634_00985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOW96340.1"
                     /translation="MVLARIPIAGRPADLPLPVCAHLQDAAGGEYVLTRATESELEAA
                     GRPHEVLDPAADGARYLLAYPRRPGAREAAAARFRILLDDGRRLLIRGATDADALALA
                     EGGCEVRWLGDRPLAFVAPRTARRLPDAPTALTASAHVRDMLARVTTNGLAYAMNELT
                     GPAACVADGSYTNIRTRYTTSGRPSWRANAYMAEYFSALGLSVTQRAWAVGGTSNRNV
                     IATLPGTTASSEVVVICAHIDNMPSGATAPGADDNASGSLAVLLAAEVFRDFTFQRTI
                     RFVLFTGEEQGLYGSEAYAAQCAAAGDNIVGVLNLDMIAWDSGSDGVLWLDTRLTSDP
                     GYAADRAIAAMFTNVVAVYGISGLAAQIHANGVEYSDHYSFWTEGYPAILAIEDDDDF
                     NPYYHTTSDTASTLNWTYFARFVQASIATVAHLVVPVDRAPFDAVRVISGPFAPTSTV
                     GHGTFVARHQAGAAEGDDRCDAGWSNAPVFPLTNRLALMSRPGGTNLYMDARPQGSET
                     IFFANLVSIQTNASLATTNRLRFDWVGGADTNAAYLVRVVVTGQYVAGGSTWTCVTNL
                     RELMAAGGFLSVPASLQVTNRAVYGSCEIRRLGVDREAVLAWAAAVPTNPVLRVEGPP
                     GIQVADLLEWSGTLTNWTAMAVVTSRVAATAATFASGENPLYPSTPAPPADGQPRFYR
                     LRRRWILP"
     gene            41362..41934
                     /locus_tag="PK634_00990"
     CDS             41362..41934
                     /locus_tag="PK634_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOW96341.1"
                     /translation="MKKINDLEAALMGLLNEKPMHPYEIEKVIVERDMRWWTTISMSS
                     VYKLLRKLEKEGRVKSEVRLNRRNLAQKVYHLTPAGRKALVARVKALASEWTRPVWPV
                     DIALSNLRLLTDEEVAACFRQYEDSLDKMIECYGRLQEYLEAHCPLPNVQLAVRPLAL
                     MRAEKQWVRGFMKQYGKKRKGGQAGSAPSR"
     gene            41964..44198
                     /locus_tag="PK634_00995"
     CDS             41964..44198
                     /locus_tag="PK634_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOW96342.1"
                     /translation="MPDFIRVVGARQHNLKNVSLDIPKHRIVVFTGVSGSGKSSLVFD
                     TVCAEAQRQLIETFSAFARRRLPKISKPHVEEIENLSTAMVIDQKRLGTTLRSTVGTV
                     TEIFTHLRMLFSRCGEPFIGFSNAFSFNNPEGMCPACQGVGKEMVVDMDRLLDREKSV
                     EQGAVLHSNFKPGGWYWKGLMKCGFFDPRKPVKDFTEEELHNLLHRAKTPFTNILRGE
                     EYTSQYEGIVTNLKRRFANRDEGEDAYTRFFTYQDCAECRGSRISEAARGVKVQGVTI
                     PELVFRELPDVLQFVESVRGPVAEPLVRKMKQSLANLIDIGVGYLSLHRPVATLSGGE
                     SQRVKMAKQLSCNLVDLMYILDEPSIGLHPRDVGSLLRMLVELKEKGNSLLVVEHDPA
                     VIRAAEYVVDMGPGAGSQGGEILFAGPVAELTTSGTLTARYLERRRDGAHARRAPAGW
                     FQIRDAAVHNLKRVSVRIPAGVLTCVTGVAGSGKSSLVHDMFCREHPEAVVVDQSPVG
                     RSSRSNPATYVGVFDAVRREFAAATGASPALFSFNSDGACPKCKGLGMVKVDLSFLDD
                     VTMTCDECDGKRYKDEVLALRHKDKSIFDVLEMTVRDALGFFEHKEILRKLRILERVG
                     LDYLELGQSLSSLSGGEAQRIKLASELHKKGNIYVMDEPTTGLHMADTDRLLGIIGQL
                     VARGNSVIVIEHNLDVIRQADWVIDLGPDGGAAGGYVVAEGPPEAIAANPASHTGRYL
                     KETL"
     gene            complement(44224..44631)
                     /locus_tag="PK634_01000"
     CDS             complement(44224..44631)
                     /locus_tag="PK634_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HOW96343.1"
                     /translation="MTFVKRFAITFVLFLLLDSVWLGVISPRFYKAHIGFMLADPPVW
                     GAALLFYLVFIAGLTFFAVEPGLRTASLGRAAARGGFFGFVTYATFDLTNQAVIAGWP
                     WIVTIVDLAWGSFICAATTLASVLVGRRIARKA"
     gene            complement(44651..48304)
                     /locus_tag="PK634_01005"
     CDS             complement(44651..48304)
                     /locus_tag="PK634_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOW96344.1"
                     /translation="MGPTPYADAAGTGYTFRVWAPNATAVGIKGEFTGWGTTALTSEG
                     GGLWSKDINGAQAGQKYKVRVNNSFDKRDPRARQVVNSAGNSVLYDPDAFDWGSDSFS
                     TPYRNELVIYEMHPGTYNAESWVPSSFDKDIERLDHLQNLGINAVEVMPPFEFPADKS
                     WGYNSSDPYAIESALGGPDAFKRFVKACHERGIAVLVDVVHNHYGPSDLDLWRFDGWS
                     QNNLGGIYFYNEYAKANTWWGDTRPDYGRSEVRDYVRDQIFMWLEECRVDGFRWDSVF
                     NIIYYNNGANHNADGEAMLRDINWEIGQSYPGKIRIAEDNAFDYSVNFDSQWHVGFNS
                     DLEYQVTRSSDADRNMSTVASLLNGWPSHNRVIFSESHDSVGDLNSNVRLPRDIDAGN
                     PDSIWARRRALLAAGIVMTAPGVPMIFQGQEMHETWTFSAEQSLRWSLTNSYSGIVRA
                     YRDMILLRRNVAGATGGLKGTGINVHHIDNANKVIAYTRWDAGGGADDVVVVANFSAT
                     VRNSNNYYIQFPSAGTWYSHFNGDSTNYQADFGNIGPASVVAAGSPPTAAVNMGMYSV
                     QIFSKEEHVTPGTVAYDPAAPGGCVEVKITYDPLDHPLKDAAQVKVYLGRNEWQDVVD
                     APMSNHVGGTWEYTHTIAPGTYRLDLVFNDGAGTWDNNLGSDWHLSVSGCGGGTPAEV
                     TVSPSAPQGCVPVTVTYRENEGPLTNAASVYLFIGHDGWLDVSSPGTAMTESPAGTWT
                     ASYSIPAGARRLNLVFHDGAGTWDNHYGQNWSVAVTNCWPAPPSIALTNPVGTISVPH
                     GTSQYTLSGTATVATAGFVAWSNQLTGQTGSGAATARWALASVPVEVGTNYIRVAATN
                     RPGNASASDTASNSVYAAGWTNRSNGGTGWGGGWTLVGGASAGHYYATLPGSSNLNLA
                     SSAWGLWANNGGLSEARRPFAAPLQAGDVVRIGFENNFIDTGRSTGWTLHDDYGNTLF
                     EFYFVGGESKYRITDAAAAQTTTVSWTDSGLLSEFELTGPTSYLFRAGGFTRTGTLSS
                     AAAAGLTQVRVWNASAGGGTPYNVYITELKITSSHAPPAGAQAVDAVTIIRPAYDLES
                     DTDGDGLPDWWESRFFNNTTGAVAGADNDGDGRLNDEEYAADTSPTNAGSFYPNVITN
                     YSRDGQVVSLSVGPPTTNSRVYDVFWSTNLRESAWNATHLDRPGASNAAAVTLTVTNT
                     RDLIFYRTGVKIP"
     gene            complement(48465..48974)
                     /locus_tag="PK634_01010"
     CDS             complement(48465..48974)
                     /locus_tag="PK634_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOW96345.1"
                     /translation="MTFLELAAKRSSIRGYKPDPVPEDWLAKVLEAGRLAPSAANKQP
                     WHFIVVREEPARLKLQQAYPRDWFGKAPAILVVCVEPGKAWVRQDGKNYVSVDGAIAM
                     DHMTLCAADLGLGTCWIGAFDPKKARDILGLPEGIEPLAMTPLGFPDAPPRPKSRKET
                     AEILHYERW"
     gene            complement(49096..50352)
                     /locus_tag="PK634_01015"
     CDS             complement(49096..50352)
                     /locus_tag="PK634_01015"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOW96346.1"
                     /translation="MSLHEEMLAMGDKAVAACRALAQLSSRRKNSILEAMAAEIEARR
                     AGIQEANTRDMEAGRVAGLSGALLDRLLLNDARIEGMVKGLRNVVVLKDPVGTRISRW
                     IRPNGLVIQKIRVPIGVIAIIYESRPNVTADAASLCIKTGNAVILRGGKEAVHSNAAI
                     AAAMQEGGAKKGLPAGSIQLVQTADRDAVRELVQMEGRVDLAIPRGGEGLIRAVAEQA
                     RVPVIKHYKGVCHVYVDESADAEMALNIIENAKCQRPGVCNAIEKVLVHEKIAATFLP
                     RMAERLAARKVELRGDAASRAIAPSMKEAVESDWTEEYLDLILAVRVVANVKAAADHI
                     NRYGSRHSDAIVAQSEAAQKLFVQEVDSATVYVNASTRFTDGGEFGMGAEIGISTDKL
                     HARGPMGLEELTTYKYVILGNGQVRE"
     gene            complement(50349..51488)
                     /gene="proB"
                     /locus_tag="PK634_01020"
     CDS             complement(50349..51488)
                     /gene="proB"
                     /locus_tag="PK634_01020"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOW96347.1"
                     /translation="MRATFGGTRRVVVKIGSRVLVQRDGRPDLRRLRALVGDLAVARR
                     AGREIVVVSSGAIGAGMSVLGLKTRPTHLPDLQMAAAVGQSRLMAIYDRLFAEEKCRV
                     GQVLLTHADLNDRQRHLNARNTMMNLLRHGIIPIVNENDVVSVDEIKFGDNDLLASLV
                     ALLIQADLLILLTTVDGFRAPAAGGRTRRVPFLKGVSEKALALAVGKGSELSTGGMMS
                     KLQAADTVAGVGTPVVIANGRQAGAIGKVLAGDDIGTLLAAGKPSGLSGRKRWIAFFH
                     KAKGSLVVDDGARLAVVEKGRSLLPIGVKAVEGEFEAGDVVNIRAAGGPAFARGVTDY
                     SSGEVRRIQGRKTSEIAAILGAADFEEVVHRDNMVVLQGATRGTI"
     gene            complement(51906..52232)
                     /locus_tag="PK634_01025"
     CDS             complement(51906..52232)
                     /locus_tag="PK634_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96348.1"
                     /translation="MGRTKGSAPRSAQDIDLAFSQLRRLGVVECAQANGVASGGLVPN
                     DSLYSSQWHHGIIGATQAWDFATGTAAITIAVVDTGLCSTNSDFDTPALPMDTTTSTM
                     TRSRRT"
     gene            complement(52472..53038)
                     /locus_tag="PK634_01030"
     CDS             complement(52472..53038)
                     /locus_tag="PK634_01030"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOW96349.1"
                     /translation="MKESTSVPLRPTLWRTCRVLANPRRLAMLQLLVARGELDVSGVA
                     RLMDCSLGTASQSLRMLNARGLLNVRRNGRHVFYRAGGDRSLPESALLLQAVVSALGK
                     SGASIDALLKTLTAATHPRRQQILKWLRRPMRYGDLKARMRVSSAALTRHLQKLRARA
                     FLDTKSGWYRLAWPADDLRKALLALAAR"
     gene            complement(53233..54669)
                     /locus_tag="PK634_01035"
     CDS             complement(53233..54669)
                     /locus_tag="PK634_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW96350.1"
                     /translation="MKSLGWLFDKAPTPGSSEEIEAARRRTMKVSMVEGSIWSLMSGF
                     GETYIMPFAVFLKAGNRAVAFLGTMPALIGAISQMAGAALTDRVGRRRDWIVLFCLIQ
                     ALVYVPLFFIPRLFPQFDVLAVIVFAALAIGAGNAVAPAWTSLMSETVPPAERGDYFG
                     RRGRVVILMVFMSTLAAGGILSLYQRIGMVWAGFGVLFGVAFMARLVSVQLLTRHYDP
                     PYRPTPQDYFSFWDFLRRIRHSNFTRFAVGAALMNGAISVASPFITVFFLRDLKWSYA
                     EFTINAAAFQAVQFLLVRWWGRMGDRHGNRSVIVATSCLLPLIPLLFALTTNYWLLLL
                     IQMFAGVAWSGFAIATQNFTFDAVTPPKRARVTAYMTILNGACTLLCGTLLGGWLASR
                     LPTHYRFGGWNINLLSPLPALFVVSAFLRVLAALLLLPSFKEVRAAEPIHPAMLLLRL
                     SGGEAISGLLQEAVARLPLPRRKVRARE"
     gene            complement(54676..56193)
                     /locus_tag="PK634_01040"
     CDS             complement(54676..56193)
                     /locus_tag="PK634_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOW96351.1"
                     /translation="MAWSSKIQDKLQALPDKPGVYLMRDRRGKIIYVGKAASLRHRVQ
                     SYFRQATLRSAEPKLRGLLRSVEDFDILVTRTEAEAILTEGRLIKDYRPRYNVLMKDD
                     KRFLLLRVNLNEPFPRFESCRIQKEDGATYFGPYASSPAVYAALEFVERRFGLRVCRP
                     RVPGPEDHKHCHNDIVRYCSAPCILKVSPEQYRERVETALAFLRGERPEFLKEMQQDM
                     EKAAAELRFEKAAALRDTCLLLRRAIRERARGRKSPVRKATEAGAGLEELRRVLALAA
                     PPSVIECFDISNISGTHAVGSMVCAVDGQPAPNRYRMFRIRTVEGSDDPAMMAEVIRR
                     RYGRVLAEKMPRPDLVLVDGGLTQLRAARQVLDELGLADLPAAGLAKRFEELYADVRS
                     NTAPLVFASDSPALRVLQQIRDEAHRFALTYHRRLRLRRIRESILDEIEGVGDNRKEA
                     LLKHFGSVLRMRKAPIEEIAAVPGIGTALARLIHEALEGGSSSPPDDTGGPPVSP"
     gene            complement(56201..56902)
                     /locus_tag="PK634_01045"
     CDS             complement(56201..56902)
                     /locus_tag="PK634_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOW96352.1"
                     /translation="MNQSRSLPYPAIQIAGVIDRAEAELLVRLGATHLGFPLRLPVHK
                     EDLSEAAASEIIRRLPPTCPGVLITYLERADEVVEFCRQLGARHVQLHGGLAPDQAAR
                     LRERDPGLFVIKSLVVRPDNVGALVAQVRELDPFVDAYITDTFDPRTGASGATGKTHD
                     WAVSVELVKLSARPVILAGGMRPGNVREAVRRVRPAGVDAHTGVEGPDGRKDGALVRR
                     FIEEARLGFAELARE"
     gene            complement(57033..57590)
                     /locus_tag="PK634_01050"
     CDS             complement(57033..57590)
                     /locus_tag="PK634_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOW96353.1"
                     /translation="MKANVATAVCVAALFSVVGLEVAQARPWWGARRRHADKNNDGVV
                     TPAERHVERRWEARHRAKVNSAWERKVDVNNDGWVEPAEVHAWRYKVLDTDHNGVITP
                     DENRAYWAGWKGVVNTDIERKYDANGDGYLEWSEGRELLKDRLRVIETDGRAVVNTDI
                     EREFDADGDGVIDRDEADAIREVLD"
     gene            complement(57682..59358)
                     /gene="ettA"
                     /locus_tag="PK634_01055"
     CDS             complement(57682..59358)
                     /gene="ettA"
                     /locus_tag="PK634_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOW96354.1"
                     /translation="MAGEYVFNLVRLTKQYNKVNVLDDVNLAFFFGAKIGVIGGNGSG
                     KSTLLRILAGMDRDILGEAHIARNAKIGHLPQEPPLDPSKTVEQVIAEGLAEPRAVLA
                     RYDEICEKLGKSPPAAEAEKLNDELGRLQDQIDAHELWEIDHKLDMAMEALRCPPPET
                     SVTTLSGGEKRRVALCRLLISNPDVLLLDEPTNHLDAESVAWLEAHLKQFPGTLVVVT
                     HDRYFLNNVTEWILELEGGHTFPYKGNYEAWLAQKQALLAAEKKQAAARSKVLEQELQ
                     WVRLNPSGRQAKSKARLKRYEELASQQVDVREDSLEIQIPPGPRLGDIVVRAEGLTKS
                     FGDRLIMENVTFDLPRGGIVGVVGANGAGKTTLFKMIVGQEKPTAGKLAVGSTVVPSY
                     VDQFRDALDAERTVYEEITGGEELVDLGGKRMNGRAYCARFNFLGPDQQKKVGKLSGG
                     ERNRVHLAKLLRRGGNLLLLDEPTNDLDVTTLRSLEEGLLNFGGCAVVISHDRWFLDR
                     IATHILAFEGDSQVRWFEGNFEAYHEFRRKTLGDAADQPRRIKFRPVTHA"
     gene            59472..60284
                     /locus_tag="PK634_01060"
     CDS             59472..60284
                     /locus_tag="PK634_01060"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOW96355.1"
                     /translation="MDSPQITGRGNAAAFTLIELLVVMAIVIVLAVLAVPWALRSINA
                     ARERACAANLRAWGQAFMVFAAEHQGLLPHPDDRRRDGGAPGSSSHPEHDQGYIDLLP
                     PYMGERPWRDIPEGEKPRGGIWQCPAAKPRPDSEYDYKPSRQGYHSYAMNSYLAHDFY
                     FGLPWDAELQPSFLYLPRAQNTAATILMFEQTLDPGQGYGQAGSFRSAGYQTAEDARA
                     VTERHRRSAEGLGGNVLYLDGHAGWRDDLWDETTRNPRIPKRGDLTWFPYEY"
     gene            60304..61071
                     /locus_tag="PK634_01065"
     CDS             60304..61071
                     /locus_tag="PK634_01065"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOW96356.1"
                     /translation="MSRKTAWFIACGMAALAAGPASTASMIYEITTDTYLDSASPTYN
                     YGARNVDRVVINASSAARTLFKLPAEVWSYAPIQIVSAVVSFYVWSDNAQTYDVSLHP
                     MLRDFAEGTGTGSATGDGATWNTYDGVNAWTTAGGDFDSGYSVLGVKGTLGVNPGEPN
                     GRFYTWDITALLNDVTAWSELQSYGAMLRIDETVPPSGQRFNAYTSSDSTTYTAPYLP
                     SLTITAIPEPGTLALLAMGLPVGAMLLRRRKQGILSC"
     gene            61096..62007
                     /locus_tag="PK634_01070"
     CDS             61096..62007
                     /locus_tag="PK634_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96357.1"
                     /translation="MKRRAVQWAAVALAGLLESAGAATVRWPIATDTYLDSRTENQTL
                     NYGAANTLKALINNNVTGDGSVCRALFTLPAELWNYSPTSLVSVTAWFYVWQDNTGDR
                     HVTLFPLARTFVEGTQNGGAADGATWLTYDGAHAWLQAGGDFLTNFPAAGVKEEILDE
                     GMHDRFFSFDLMPLLTNETAREALRQNGALLRIDEQPIPTNGLPRAPFTSSDDPGYTA
                     EYKPHLRVEVAPPEPEIGGPTMNGGTVTLPIGQLTPGITNTVERSYDLAAANWTSIAV
                     FVTGGNATNWSLPLSPSNRVFYRVTTR"
     gene            complement(62090..62470)
                     /locus_tag="PK634_01075"
     CDS             complement(62090..62470)
                     /locus_tag="PK634_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOW96358.1"
                     /translation="MTTKRHTLKKIFGQPEARPRLRLICATIGPGKAHLLQLIAETQS
                     VSEAARRMGIGYKQAWQMVAEMNAVFRGPLVESISGGARGGGSRLSVDGRKVLVLYTS
                     IQERCDTAAGREMRELYRLLKTGV"
     gene            complement(62478..65480)
                     /locus_tag="PK634_01080"
     CDS             complement(62478..65480)
                     /locus_tag="PK634_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96359.1"
                     /translation="MKAATCFWMGALLLAAPAAGRAQDMPIGERIRRVESAHFVVYYQ
                     ESLADRAPALVRECEAAHAVLSPIFRWAPREKAQVLFHDGWDEHNGWAFTIPRARLSF
                     FAAGPSPSSIYEPGGYLRSTVWHEYTHVLMLDAKHGFGNWLGRVFGRFLPEIGDPVST
                     LLFYFALPPGLTAPNWFLEGASIWSETEFTRQGRGRQSLPNMVMRMAVADGRALDARQ
                     WELAHPEWPYGDAAYLWGARAVQRAQEDAAGPKDRSVVGELADSVSRSWPWFFDDRAR
                     PVTGESFADLARRALDEETRYQRKQLAKLREKPLTELKKRTPDRLLVSQPKFSADGQS
                     IFFAGNREAARDVLFRYDLESGRIQPLKHARVQAGISRLAAAGDRLYYTRLNVRGRDR
                     MYSELRAMDAASGRTRAVSAAGRYRFPAISPDGKKLAAVRDEAAVCRLVEVPLASAGR
                     RDRERVLAEAEPFNMLVDPVYSPDGQWLVFVQGREGESELRRVNLESGEGSTLLQWPC
                     IVLGPAFHPDGKELVFSADRSGVYNLYRLPFKADAEPEILTHVEGGLFEPAFSPDGTK
                     LAAAAYDSYGYYLAVLDYGNLKPIAGALPTIRREWPAVAENQRRLKEAEKAAPEPGPA
                     RPYRSLLEARLDYWTPWAAATEESAMGGLAARLSDPAGFQSLSLLGGYDGEEEIPVGS
                     AVYSYAGMYPVFTAFGLHSADTYPDLVRDTEGHPYDYEEKSGAAGLAITVPWIRADRE
                     LALTLGYRYLDREALESGETNYADRALLTTNLFEGAEAALFGQLEYFDGTAFPRSHSV
                     EDGRYISGGIEWTDRALGGDLDRVRWLVAWLEYFTLPWGENHVLKMDGIYGAGSGDDT
                     AQGFFGVGGLGDIVENSTPGLARTLALRGYEPNTQVGRQATRAITAWRFPLCRVYKGM
                     SATKPVYRQQLFGELFWDLGMAWDGEPPGQPENDWLNSVGLELNYSMTLLRFLDVAPG
                     LGVVYAFDREPSDERAQAYISLKSAVNF"
     gene            65620..67596
                     /locus_tag="PK634_01085"
     CDS             65620..67596
                     /locus_tag="PK634_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96360.1"
                     /translation="MKRIFGTGLLLVGLLGLCALADPPAELDYQGKILVSDIPLTGPG
                     YFKYAIDDLAGTVNYWAHDGTASGEPATYLTNDCYNGVFNVRLGAAPMTAIDPDIFQN
                     GNDLYLRIWFSEDQVTFNEMLPAQKLVSAPYAVNSDRIDGYDADELIAAATNAFTETD
                     PVYSSSDAAGVTASDISNWNAAYGWGDHSSVGYLTAETDPIFGASAAAGITAGHVLNW
                     NAAFGWGDHALAGYLTAEADPVWTASSNNFYTKGEAESRYVNVTGDTMTGTLVMNVGL
                     NNDLIISSAGRTIGIGNNTGVGIGNDRIAVGREVTNFVDNTARIRGTFWMDGGTGLVA
                     RPVFGTGGWKTLAPIPPFQHVIYVATNGTPGGPGNIDRPFDNPQNGYNYAAVTFTNDP
                     VALVIAAGAYGGLNMNAGNIHILGQSRAQLDTLTVSSPARIIMGKQRVESLCVKNAAI
                     VAADLGEDVKFDNCRFERGLTIYGPDVEVQDCYASAMDGSAITVGDGVNTIQNVALYN
                     SSLYNESGVMGTLLVNPSVGNFEVVGCQIVNGALGICIEDREVGPIVPVHLYSHNVIR
                     GMPPAPASVVAVLDQFAAGTTIAFVQNTVLGHVGISAHPQYYANNIVYGRINNIGGAG
                     TPGWAQAGAGVGADASNNTEHQGAFPVLPSSWSD"
     gene            67607..68122
                     /locus_tag="PK634_01090"
     CDS             67607..68122
                     /locus_tag="PK634_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96361.1"
                     /translation="MNKTLSFLAAFLSGLLPAVLWAAGGRVGGHAEIQYEAVGAGGAA
                     FASSGNVKLGGTLGQGGLALLATNDQDQVFLNGFWKSEDSCTLYNPTITQLTQSTGTV
                     AVTFMVINGNTYSVQYVTHEEGGLMLGSHAITNPVTTVVGTGLAGSTTTIWHNVSAST
                     NRVRFYVIRCE"
     gene            68245..>68913
                     /gene="manB"
                     /locus_tag="PK634_01095"
     CDS             68245..>68913
                     /gene="manB"
                     /locus_tag="PK634_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOW96362.1"
                     /translation="MAGIFKAYDIRGIYGNTLTEDTAYRIGRAFATFLKCRKVVVGRD
                     MRPHSPPLFQALARGLTEQGADVVDIGMCSTPMSYFANNRLGADASIMITASHNPGEW
                     NGFKVCREKAIPISGETGIGDIERIFHSGAFAPAAARPGAISTHEILDEYAATIRGFA
                     EIRRPLKVVVDYANGMGILEGKVLDGLMEIEPLFDTLDGTFPNHEANPLKHDTLEALQ
                     ARIRS"
BASE COUNT        13118 a        22252 c        21394 g        12149 t
ORIGIN      
        1 gctattactg gccgcccagc agctactccg gtggacataa tcagtccaaa aggagatggc
       61 gtcattaatg tgcaaccgaa tgcggaaatg cccaatattg gcgaggctgg cgacattgtt
      121 gtctatcttg aacccgatgc gggagacatg agagaagcca agcgtcaacg ctgttggcat
      181 aacgacatct tggccacccg ccatggggat ctgtttctaa tggtaacagc ggccacccgc
      241 aagactatag aacctgtttt gtgtgtggag aataaactgc tgcgcacgga caaaaggggc
      301 caattccatc tcttcaggcc ccatgcgccc acatttccgc ttaacgatat ttatcaacag
      361 caagtggacg gctggacaca aatctacacg ctggccaacc ttgtgaatga cgacacagac
      421 atggaaagtg accccgctga tttcacggat gtagaggctc tccgccgcct ggcaatttca
      481 ttattccacg tgaactccga agccgacact caatctctac tcatggtttc ctgcgcccag
      541 tgggtttggt cgattatgct tttggctttc tgcatcccgc tgaataatca cacgatgacc
      601 gagctgggag ttgaggatat ttggaacaat cggtttcgtg atatcccccg ctgtgacgag
      661 aagttccaga tcagcagcgt gttgcctatg cagccttttt caaaagacga ggcagttgta
      721 gcgcttgccg ccaattacgc tggtatgcac agtcttctat gggatcaatc gcttgtctcc
      781 aaaatactga tgctggtcaa actggaccca caaatagcca gcacccataa tgccctacct
      841 gaccggataa tttggccgac attcccgctc tgggagcatc ggcacaaacg cccgagtact
      901 ctcatggagt tcgaatatat cgccactgtg ttcccggatg aggtgtgcgt tggtgcgtag
      961 ggagaggctg ggctgtacgg cgaggcgagg gagcagcgcg ggcaatctga ttcgccagca
     1021 cttaccaaga ctgctgccga tagattgcct gtctcggcgg aactgtaata tcattgccgg
     1081 caagccgaat aaggcgctcg cagtgctctg attctttgat cccagtgtag acatttttga
     1141 ttgttagccg ccttgatttc gcatctcagt tcaggcgcgg ccctagtgtg tgattgttct
     1201 aagccattga atatgtcggc acagaccatc taaatctgga aacagcgaga atctgtgcac
     1261 gccatatttc cagagtatgt ttttcaattc cctcctgaac tcttttgcta taattatctt
     1321 tctcatgctg gctggactaa attcatcacg aggccttgga tgacatgtaa agagtcccgt
     1381 ttgaactgtg atacgattag ttagatgcct tggtatgaat ttaaccactt ctttgatttt
     1441 aaatgggttt ctgtgcctcc cgatgtcaac tatgtttcta ttctcatagc aatacagcat
     1501 actatcccca tcatgcgacc tctctaccgc ataccatgcc gccacaagag gatttctagt
     1561 ccagtcaaga agcctagtcg gcagcccatg atgttgtgcg agcgctaacc actcccacag
     1621 attatcaggc acagtattca aatacggcaa tgcttgattc tgaaacaatt tcagcatttg
     1681 cctttcgctg gattcaagcg cacctcttgc gaattgatct attctgccca gctttgggat
     1741 caggcggtaa tcacagcatc tcacgcctct gtatatttca tctcgattgt ctatcttaaa
     1801 tacttcgtgg tggaattctc gaaaagtagt tacccttgat tccatatagt ctccgcatca
     1861 ctttgagaat atatcattgc tctgccaata acaaagccca cttccccaga taatgagcag
     1921 aatgttcagt atagaatttc tttagtagct gtctactctc tgactcaccg ccacccttct
     1981 caacaatcgg cggcgtatag aacttcatga tctgtgcact atggaccacc tgcggcccag
     2041 ccatatgcgt agctccatca gttggggcct ttaatgcgta aattactttc ccgatattta
     2101 cctgcatgac cgctccaagg cacatcatac aaggttctag cgtcgtaaac aattcaactc
     2161 gtcttctgct tgaaatgcgc aatgcggcta ttgcatcatg tgccatgcgc agggccatca
     2221 tttccgcgtg gccaaaccga tccccaccca taaccctatt atagccgcac cctataacac
     2281 acccctcatg tacaactacc gcgcctatcg gtacgttatt attggcgagt gctgttcgcg
     2341 cttgatctag cgcatattcc atgtatttaa tattcatcat gttagtcgaa cgccagttgc
     2401 ttggcattat tgcgagcgac cccacgagca aggttggcat gaaagccctt gtggctgtct
     2461 ttcctcgatg taacatgctt tcacatgaag aatctgaatc cgttccgtgg gcttacatct
     2521 tgaggtatcc atttcagtat atcgcccaca cgtcgcgctg ccctgatagt gattggttcg
     2581 ccaccatcga actgggtgtt attccagttc agcttagaca gtgccatcat ctcttctccc
     2641 acttcttcaa caccattctc cgactggcat agacagaact caaaagggcg ggggacatag
     2701 agacccggat atgcctggaa gaaggagacg ctcccccgga gatagaggag ccccctggca
     2761 tcttctagct ccacaagtgt tccacggaat ggcggataaa tatcagtccg aaagaaccga
     2821 gaatatgacc gccggacatg tatcaaatcc gtgtaggcga tcctctccct ttccgacgcg
     2881 cctttgaagc catcctgctc ggcatcactg aaaggcgatg tcttgtgcag tatgacgcga
     2941 gcaggaacgg tgtgatgctc tttacggtag agcgtcagtg cagaggacag aatgtcgaag
     3001 gaatcattgc gggacaagtg atactggtgc tcagacttgt cccaggccgc gtcaccaccc
     3061 ttaataatga ttccctcgcc acgctcgttg aacacatgcg ccacacttga cgctgcatcg
     3121 ttgctgttcg gaatcttgaa aaagctgacc cccacaaaac acgcggtcag gtcgcttgtt
     3181 tgccttggga tgcgccagag cgtgccgccg gccttgtagt aaagagcgac ggcgaagttc
     3241 catgcacgcg tcgcctcgtc ttggagtgtc ttccggcctt tatgcccctt cttgttcccc
     3301 gccgtcatgc cataggtcct tgggcggacc atctggatcg gtacggctaa acgcatgcct
     3361 ttagctttga ggatatcgtg gaagcacggc gttgcagcag agggagtggc ctctgtgctt
     3421 tcatccagtt cttgatcatc tgggtcaatt ggcggcctcg gatctgactc aagagcggca
     3481 aacagctccg gaggaggcgc gacgataagg acactcggac cgcctgcatc cacgagatca
     3541 tccgcgtggc tcaagaagag gtcaaccgat cgctctgcaa gttcagcggg agtgcacgag
     3601 gaaatcatgg agtctacttc gcgcatgctg acgggagaga tccacctgtc gtggatctgc
     3661 agttcggtcc cgaagcatga agatgcgtct atccccggga aaggtggatg taagttcgaa
     3721 aggtgagtct gctgcgccgg aatctcaccc cgcagtttct gtagccactc gcaggctgcg
     3781 tcacacgtct tcttagttcc cactactcca accttcaagg gacgcggagc cagatctgta
     3841 tcacggtcca acgggccgct ttccattagg ccaaacctaa tatccacgtg cgtgcctgca
     3901 ccaaattcga gttccggttc ttccagcaag atcgctctca tgacatgacc tcatcaccaa
     3961 agggaagttc cataatgcta tcatcagaat cctcgcttgg gggctgcggt ggttttcccc
     4021 atgccgaatc ccgcagtatc gctggcaact caatctcaac gggacgcacg aactcaagat
     4081 gacggggaac ggatgtccac atatcgtccg tttcacacag gaactcagcc cacaccagga
     4141 cctggcttag aacagctcta ttgccctcga tccgtttgat ccctttcaac tgatcttcgt
     4201 gaaatcgata gagccagttc ccgtcgcgcg tgaaccggta cgtaggagtg acctccaaga
     4261 accacttgcc ttggagtttt cggaaacgcc cccggaacgc taagtgcctg taatggacga
     4321 aggtccgacc atcggccgtg tcatgcgcga actcttcgac aaccgtaaca tggctctttt
     4381 tctctgctga ctggtaaggg aatacaactc gacccgtcca agggccacca acaaaagcga
     4441 agcaatcctg tttcggccag aaacggaccc tcggactgag ttgcgatttc agtgctcgat
     4501 taagcaattg cacgaaaagc cgttggctgt cagcattact agagtccgcc cactcagcag
     4561 tatcgaacgg ctccacagtt cccttgtcac agatggcgtt ccactccggc gatttgaggt
     4621 cagcaaaaga gaatactccc ttgtcatgga gcacccaggc accatcgttg aaatcagcag
     4681 cctcgcgaag tgatcgccac acatcgatgg gataacgcat atccgtcatt ccatgataga
     4741 tcgtgccgct aaaacgacgc agaggaaaga gatttgagac ggctcgttca gttccgggct
     4801 ggcaagcttc aatgaaggtc ttcccgccaa tcttcggggt gcgcagcccg cgcaatgcag
     4861 cccatgctga agcggagaat gcgtcggctt taacgtcaaa ttgaatcttc ggcgacacat
     4921 gtcgcgagta atccgggaac cggtcgtgaa tacatgccca gtacgcctcc ggagtatctg
     4981 ggtttgagac aacgagaatc actggaagat tctgccgcaa ccagtactcg atgtctcggc
     5041 ggtcacacca gtactcgatg gtgtcggtgc ccttccggca aagagcggcc agggcctttg
     5101 attgaaccgc tatgttcttg cccgttggct tgccagtcgt cgaatcaaac agttcgatga
     5161 aaccatcgat gccaacctcg ttagggccgc ttggcgtcca tctggacttc atatccagaa
     5221 caaccctctc tacgaggttg acaccttctt ggcccctgac ggctttcctg tgcaggttct
     5281 ttgtcattga tttctacagc cttggcagaa cgccatggtt tgcttcctaa tccgttttcc
     5341 tctaaaactc cgccaagcac atcataacgt cccactattt cagtaacaga tatctcaagc
     5401 caacgtcaca aatcagcggc agtgaacccg ccgcgggctc actgtgcgcc ggattatttt
     5461 gttggttgct tctttgcgca ttcctctgct ttgagaatat ctcgtatcca agcacgaaca
     5521 atccgcggta gttgccttgt aacttggttg aggtcgaatt gcttgtatag atagccaatg
     5581 aggtctgtct gcaatcgtgg caagaaggag tccttcagaa taaggacgct acgcccgcgt
     5641 gacagacaga agccgagttc aagcgaaacg ttcggattga actcctcctg tatagtgctt
     5701 tctttctcta tacgagtaaa cacggctata ccgtacttac acgccagaag gaatgaagcg
     5761 acgttatccc acagttgagg ggctaccgtc ttgtccgagg ccaaccacgc agtgaacccg
     5821 tgcttcttga gctcttgcct gataatactc tcgattcgct catagcgctc gtcgcgatcc
     5881 tttcgaaatg gcatcatcac aaacacgttt ctttcatatg gactttcttt caacaggacg
     5941 cttgcggatg ggtcgagatg agagaactgc gcttgtatag ccccatattg agcgtccatc
     6001 cgcttaagtt cggcccccag acgttgagct gtgtggtaga tcgtaggaaa cgaccccatt
     6061 actactgacg caattgcatg agacatatct ttgccgcatc gcatgcgaag gtagcggata
     6121 gaactaatga gtgagcgaaa gcacagttgt aggtatgttg attcgtggta cttcttacgc
     6181 gccttaagaa acactgacgg tagcgactgc ggggaatctc gaagactgaa cagtgccttc
     6241 agtgcatggt cttccgcaaa ttgctgggta gatgttggct cccacggagt tttcgtcagg
     6301 ttgcccaata cgtacgccgt atataacgcc tcaccatccc ataagccgtt gtcagcctgt
     6361 gccttgatga tctccgtcag aagtttcgtg gctgcggcag atggccgctt gccatgagac
     6421 ggcacatgtc gcaattcaaa gaagaaaccc ttgaacgatg tacgatgaac atcctcccac
     6481 tgaccttcaa ttgcattcag cgccttgcgg ataggtggcg aggtcgcttc gtgtcggccg
     6541 agcaattgaa gaatacgaat cgcccaaagg gtagagaaga tatttccccc atcaagaaag
     6601 ccggagtata gctcaaagaa gccgcttggc ttctgcttcg cgaggatggc ctttgcaatg
     6661 tcgtccctcg tcgtatcatt aaaggcgccc gctcgcgcaa gagttgtgac cataaatggg
     6721 ttgtgagggt tgccatgtgt ttttgcaaac cattggcaag cggtatgaat aatctgcttg
     6781 aaaagagtgg cgtcaacctc aacacataat tcgaccaggt gatgggtcgc cacataatct
     6841 tcgatgaccc ttccgtccgc ttctcgcgtg aggacggctc ctccgggtgt gatctctggc
     6901 agaacgacgg tctggaaaat cccaagaaca tgggtctcca ggttggattg ttccctcttt
     6961 gttagcatta gggcctccag ggctgtgtgc gtgcgttaga tcttctttgc ttttgcctca
     7021 aacgccagta atggtatcca agtgcagtaa gcaggcaggc cttggaattc atcacagcga
     7081 tgtacatgtg ttcttcactt aacaccatca catcctttgg tgcgctcgcg gcaatttgtc
     7141 ttccacttgt tgtcccaggc attcttggct gacatgtcgg tgtaactgga tggcgtatct
     7201 gtattcaacg cctgcccttt gagcagctag cgtgaacgag cgtcctcaat agcgaaactg
     7261 agggatatac gttcgactgc tatttctgca tcctcctttt tcttcgtcgt ttctccagtc
     7321 actctcaacg gtatgcagga cagcgtttac actccccgcc cctaacacat taccccgccc
     7381 ttaactcgga tgaaatataa tctccttgct ataggcgtga ggctaaacca aggggcctcg
     7441 ggttgcaaga ctaaactgcg atatccctcg tgtttaaggt gctcctggcg attcagtact
     7501 agccggatat gcgcaggccc tagcatcagg tatggtatac gggatttggc agggaaatgc
     7561 tggcttagaa aacggaaatc aggctcggtg cggagaagaa tcatggggct caagtttttc
     7621 cccgaatgac tgcggcccag atacagattc aagcccggcc cttacttctg ccagacgatg
     7681 ccgtacccac ttacgcccgc tttccgcatc aacctctccg ccctttccat ctcccccagc
     7741 gtcactgtca gtatcgcctg cttattgctc gcgaccaccc ccacaaggaa ttgctcggcg
     7801 aggcggtcat cgagcatgaa taagatgtcg tcaaccaaga gggccgtttt ttgggtcgtg
     7861 gatttcacaa gaagcatgcc tactgtaaaa acgacctgcc cgtggctcat ggctgtgaaa
     7921 gatacgggcg gcggtgctgg aagttcgatg attccagcag aagttttcag cctttccagc
     7981 agtcgtgtcc gaccagagcc actttcaccc acaaggaagt tcaagcgtgg gccaaaagat
     8041 atgccgtcga aagcagcgac tttggggatg atatcgaaat gctccttcac gtcatctgat
     8101 atctctggcg ggaagtgctg gcgtaaccac gactcgatac ggcaggtcat catgtccgcg
     8161 cctagacttt gaagaccgag caccaagtac cggttttgga caggagatcc agcaagcgag
     8221 agttcagctt cagccagaat cgttcactcc ccacaaggtc gcctgacaac aaacggctaa
     8281 gaaatgggcg ggaaaatcca ctggcacgac aaaggtccgc ctgcgaaagc gaaaataaag
     8341 tcattaaaag cttgaggcgg tctaggtttg aatctgtacc atgggacgtt cttgtaaaca
     8401 cgggtaaaca cagttcccgt ggcctaacgt cttcctggtt taccgaggct atcgtttttt
     8461 cagcttgggc aatcatttta tcacctccgt ctatgatttc gaggaatagc atatcaggcc
     8521 agagccattt ttggaagtaa attcgaccgc caccccgcac ttttttgtga ccagtaatag
     8581 tagcgcaacc ccgcctccag cctgatcacg gttttgatcg cctggttacg atcggcacta
     8641 cagggcgttt tatcctgcgc tactattact tgcagaatat tttcctgccc aactatcggt
     8701 aaagatgact ttttttactc taaagaaaat agagggaacc gcagaatgat tcttagaaat
     8761 attaaaagtg aactataata gtcagtaaca ttggaggagg tgtttttgga tggacagaat
     8821 aaaaacagaa ttgaaaaagc gaaatggcct gacagtgcgt tggccggaca gtgttcacga
     8881 ggccgtttgc gatatagcct ggaaacaacg ccagaccgtc agttcactga tccgtggagt
     8941 gatgctcaga tatttaaaac agaacggggt tcgtgttgat gagattgagt aatgttcagc
     9001 atattctagc tatttctatt taggtataaa tatttcttta tgatttttct attttgaggt
     9061 ataatggaag tggtgaagat gatgatttta agattatcgg aggacctggt gaagccctca
     9121 gctaggtcca atagccaatg gcgagttccg ccaaaggatg ggtcgggggc agcggtcaac
     9181 aaaaggatca atatggagag cttatatgtt gttaaggcag gaagccgccc gaatagtggc
     9241 gggaagctat ttacctcaat caagtaatgc tacaagcgat agattatctg taatagatag
     9301 tgattggcct gagcagtcga gttgtctgta cacgtgtggc agttcgtggc aagttgacgc
     9361 ccccctcctt cgccccctcc ttttaatggc tttggctgcg cccttggcgc gacgcatagg
     9421 cagaacgtcc accggcaggg tcgagagccc ccctcttggg cggcactcca cttccggtct
     9481 aattcacatc gccttgcagt tgtcgagact taccgccgct ccaccttgca gcactattct
     9541 gaccagcatg aaaagatttg atggtgagtg tttaacaaat ggttgggtca ttcaccatcc
     9601 gataaacaat accccaacac aaaaggagaa tacatatggc aatattgcca gaagttagtg
     9661 gtaacgcaaa ctggaagact atcccgaaag gcacattcct cgcaatttgt gaggatataa
     9721 aagacgagtt cggtgttcat cgaaagaagt acaactccga agaaatggaa gtgattgatc
     9781 agacccaatt ccggttcgtc tttaaggatc cgaagtcagg tgagatattt cggataacat
     9841 ctcgcaagat gcggatctcg tcacacgaga aatcatccct atatcaagcc cttcatgcat
     9901 ggtgtgggga gcccccacga gctggctggg acttcggcga gcttaagggg tgtggagcga
     9961 tgataaccat cgcccatatg ccgtcgactc tacatccagg ccaaccgttt gcgaccatcc
    10021 ataacatcgg cccggtccca gaggactgga atgataagtt ggaggcgctg aaaaaggctg
    10081 tcgcagatcc ggccgctaaa aacggcgtca aggaagcggc aggggtaaac atgcccggcg
    10141 agggttggtg atacaaggag gaagacggta aacgacaggg catatgagcg aggtttttac
    10201 aataccagat gaaataccaa gccataaact ggccgcagca cgtctgtatc gtgaccttgg
    10261 gtgggcggtt cacccgctac atggccctag cgagggcggg ccaagcgaac ggggcaaaaa
    10321 gccacgactt cgggattgga aatgttggtc tgttgaaaaa gtgacggatg aatttctttc
    10381 aacgtacttt agtaatggca caacctccaa tctcggagtc gtggtccgcc gtccatgggt
    10441 cttcatagac cttgattcga aaaaggacca gggtgctagc gtgcgggcgt ggttagatga
    10501 ccagcccgcg caagcgcatc ttaagaatgt cccccgctat tccaccggca atggcgaacg
    10561 gctcgtgttc gtttgctatg atctgcccca gttcaagaaa tctaatggga agaggtatga
    10621 gaaggctcta atttcccaca tcaacgaaaa gcttacgata gagctgtatt atgacggact
    10681 taacatcgtg ttgccgccct ccattcaccc aaacggatcg gcctacgagt ggaggaccaa
    10741 gggagaagtt ccaaccgtaa catggtcgca actccagcaa tggttcggat tcaggctgcc
    10801 taccgatgag ggagaaacaa ccgacagcca caccgttcgc agacagaata aagcagcgtg
    10861 gtgggcgaaa taccagggcg acctacgcac gctagatata gcggaactgt ttcatgacgc
    10921 agggatgctt ggcgacctcg tcacgccgga tgaaaataaa tactccgccc tgtgcccttg
    10981 gcgaacagag catggtgaca aggcagaggc tgccgacgac tccagcactg taatctttgc
    11041 gccacctgac agtatgccgg gtttttcttg cctgcacgca cattgcgctg agcggcatct
    11101 cgccgaagta ctggattggt ttgaattagc ggaaccgggc tgcgtagacc tgcactgtaa
    11161 acggatgaga gtttttcagc cgggtcaaac tgctcaggac gggcggccgc gcctcatcct
    11221 ccccggtgta gggcgctccg cctcagattt tgcaaaagac gcaggagcag cgattgccac
    11281 atcgactagc gtcaggcgtc ttttccttcg taacggcctg gttgtggaaa tcagtcagat
    11341 ccaattcgca aaaggacaac tgtgcctagg atttgccgtc gttaagcccg ctaggttgat
    11401 taccttgatg gaaagttacg ccgaggtggg gtttttgacg aaggacgatc tgggcaatga
    11461 cgttttcaat gcccgctcca tggacaaaaa cacagccgag gtagtattgg cagcacaaca
    11521 atttcgggag cagctgcctg ttgtcgaacg tatcttggat gtgccgattc ccatcctcac
    11581 gccggagggg ataatttgct acccgtccac cggctacgat ccgcgcttca tgacgtatct
    11641 tgccgaggac gccccgaaaa tcgagcgcat gccagtggca gatgccctgg ccttgctgcg
    11701 tgaattgtat tttgagttct gttttcggac ggcgcaggac attacccatg ccatagcggc
    11761 gctcattacg cctttctgcc gcggcctgtt cgggcgatgg actgctcgga cacccacgtg
    11821 gttccacggc gcaaaccgcg cacgagcagg caaagattgc gaggccaatc ttgcgtcgat
    11881 actctatgag ggccgtacaa acgaggatgc cccgctaacc gcagacgtgg ccgagacacg
    11941 caagaaaatt acctctgcgc tgatcgccgg tcggcgtcgg attcacttcg caaactgcaa
    12001 ggggcatata aacaattcgg cgtttgaaag tatcggcaca gccgaggtat ggacggaccg
    12061 tgttttagga ggcaatacca cgatcagcct tccgaatgag atggagatca gtttgtcagc
    12121 taatacagga ttgacttaca cgccggacct cgacgctcgg tgccgtccga ttatgctgtt
    12181 ctacagcgag gaggacccca acgctcgaac cttccaaagg ccagacctgc atgggtggct
    12241 gcgcgcaaac cgtggtcggg tcctctccgc tattgcagcg ctggttgacc actgggacat
    12301 gcagggccgc ccgtcgggac ccagcccatt taccagctac ccgctctggg caaaggtggt
    12361 aggtggcatc atgacttctg ctggattggg cgatccatgt cttcctgttc ccaatgacgg
    12421 gacccaaatc gccggggacc agcaaaccgg cgacatgaag acgctatttg cgctggctca
    12481 cgagcagttc aacggggaat gggtgaataa gggccgtatc atggaactgc ttgagggcga
    12541 gggcacagag ctgttcgggt ggctcgacct tgaaagccgt ggcggacgca catccttcgc
    12601 cctactgctg aataagcatg tagggcgtgt tctgggcggt atccgattgg aacgcaatgc
    12661 ctctgccaag aaatctcagc agcatacata tcggttcgtg aaagatgcgg gcggttctac
    12721 cgtaaatgcc cgcgccgcca tgttcgatac cgtttttaac tctgccaagg tcggagaatc
    12781 gccttccgga ggtggacacg ttgggcacct tgggcacctt ccctatactc cctatattaa
    12841 aaccaattct tcaaataata tagagatgaa aaataaagag agggaaatat ataagagcat
    12901 agaacaggtg cctaacgtgc ccaaggtgcc cgcatcttcc acaatcccct gctttgacct
    12961 ggtgacatcc tcggacgccc taaaaggcat agccgaccag attacggcgg catcggaacc
    13021 cgttgccttg gacatagaaa cgtatggagc caagggcggg ttagacccat ggcagggcga
    13081 tatacggctc ctatccttgg ctatcccagg cgtttcccca tgggtgctgg acctgaaagc
    13141 tattggctac gatcttggac cgctcggcgc ggtgctgccc gccaagccgg tcttgggcca
    13201 caacatcaag tttgatgcgc tgtggctgcg ggttaaatgc ggcttggtgc tccgcaatgt
    13261 gctggacacc atgacggcca gtcgcttgtt gaccaacggg accaaagagc cgaacgacct
    13321 cggggctata atttcccgtc acctccaaat agacctgccc aaggaccaag ggcgatctga
    13381 ttggggggca atggcgttgt ccgaggaaca actgcggtac gccgcagacg atgtgcatca
    13441 tcttcatgcc ctacatggaa agctccaggg cgaactagaa aaggccgggt tgctgacggt
    13501 gtatgaaacc gagatggacc tcctgccggt ggtggtggat atcgaggctc gggggtttgc
    13561 tattgataag gtcaggttgg cggtcctttc caagaaggcg gcagaagagc gagacaccgc
    13621 agctacggtg ctgcgacaga cattcaatgc ccctgccctc aacccggctt caccggcgca
    13681 actcaagacc gtgctggccg gtgtgggtat tacattggaa tcaacaggcg aagaagcctt
    13741 acaggccagc ggcgacgacc ggtttgtacc tcatatcctg gcttatcgca acgcccagaa
    13801 acgcaccgaa caaacagaaa agcttcttca aaagataggc ccggacggac gcattcatgg
    13861 gcgttttgaa cccaccggaa ctgaaacagg cagattctcc tccaaggacc ctaacctgca
    13921 gaacatcgcc cgaggcgagc tgcgaaactg cttcgttgca agcccaggct gctccttggt
    13981 tgttgccgac tactcgcaaa tcgagttgag agccgccgcg gccatcgcca acgaaccaaa
    14041 aatgatcgag gcgtatcgcc aaggggctga tttgcacaag cagacggcga gccttgtcct
    14101 gggcatcagt cccgatgcgg tgtcgaaaga ggatcgacaa ttggccaagg ccgtgaactt
    14161 cgggttgctc tacggaatgt atgcggcaac attggtgaag tacgccaaaa ccagttacgg
    14221 cgtaacgctt actgaccaac aggccgttca gatgagagag cgatttttca atgcctaccg
    14281 agggctgcgg cgttggcacg acaaagcagg gcttgaggct ggtggtgcca aggaagtccg
    14341 aacgctactg ggccgcaggc gcatgctgcc cgccggattt gacaaaagat gggcgcgatt
    14401 ctctacgcta ctgaacacgc cggtccaggg gagctgtgcc gacggaatga aaaaagcaat
    14461 tctgcttgtc gccagccgcc taccgtacgg ggcaggaatc gtctcaaccg tccacgacga
    14521 actgatcgtc gagacaccat cggagcaggc tgaggcggta aataccatag tgcacgacgc
    14581 catggtcgag gcgatggcgg ccattttccc gcaagtgcca atcgtggtgg attgcaaggc
    14641 gtgtcagaca tggggggaga agtagatgtt ttttaaaagt ccatcatgca gagaatatgt
    14701 tactaattag atatggctat ttagaataat ttagttgttg acatttgatt gagtttatag
    14761 tagaagagaa gcgaataata aattatatta ttcacgggcg ttgcatgcct gatctgatgc
    14821 gtgcaacaca attgatagtc ggcgcttttg ttggtcctcg tgactaacga ccaatgccac
    14881 ctcgatcaga tcggtagcgg gagcggtgtc tcccggaggt ggataaatgg aaaaatacag
    14941 gtcgttagtt caaattcagt ttgcgggagg tcttgggtcg gatctgttct ggcacccgag
    15001 ctttctgcgc aaatcagacg ctgctgcccc tctttatcaa agtcggtgcg caaaacagcg
    15061 aatcatatgg gccgaaaagg agtatcggcg cacccaccca aaagctgcgg acatcttcgt
    15121 ccagagcgaa gcttctataa cccgcaaata cctcaagcag cttaaatccc gtgggttata
    15181 tggggttctg gcagctcatc ttttccgtgc ccagaaatcc tctgctcggg ccaagctcta
    15241 ccgtcatggc gattacaagc agtacgccta tgaccgtaaa ggccaaattc ttcagaaact
    15301 gtgtgaattt ctgccttcgt tgcctgaaat caagtgggga tgggggcagg atgacaaggg
    15361 ctatctggcc gatgtcctat atatagagat ccccaccggc caggtcagtt ttcattcaga
    15421 atcacgctat gccgggccgg actacccagg cgcatgggac ggccagcggg ccagtgagca
    15481 caggattatg gcgttttgcg attcggtcat gggggcatta attgagcata tttagaatca
    15541 tggggacgcc atcatatatg agaggttact ttttaggcgt gaggtcattg acgccctaaa
    15601 cagaacaggg gtactactaa tattgggaca atgcagacta acaacaagga ggggagcaag
    15661 gacggaaaca cagcagcata gtatgtagat cgtcggagac agaatattca ccgcctcaaa
    15721 caactaaaaa ggagcgcggc atgtaaagta attcggaaat atattggaga gaataaatga
    15781 agaagtcaaa acccgctgga gataatccgg caaactcaga tgcagataat aaagtatgca
    15841 gaaataaaac agcagagcat gcacgtcagg tattgaccag tacagggaac aacagttggg
    15901 ctaccccggc agtgtggttc gagtatttga atttggagtt caaatttgac ttggacccct
    15961 gctgcaccca caagaccgcc aaatgtaaaa agcactacac cccagcggaa gatggattgg
    16021 cgcagtcatg ggcaggggag agggtcttta tgaatccgcc ctacagcgcc gaactcccaa
    16081 aatggttgag aaaggcctac atagagtgtc gggacaatgc tgccctggtg gttgctcttg
    16141 tacccgcacg agtggatacg gactggtggc accagtacgc agccaaggca tctgaaatac
    16201 ggttccccat aggacgattg acatttaaag gcgctgaata tccggctcca ttccccgtgg
    16261 ccatagttgt tttccggcca cgtattagca atccatacag cgagccgatg tgggacatgc
    16321 cggatgatac aacgcaagac gagtattacg catggcttga ccagcaggaa cagggcggtg
    16381 cctcatgaaa aagcacctct cattgttcag gtatcctggc ggaaaataca gacttatcgg
    16441 ttcaatccga gaaaagctcg acccgttgtt gcgtgatgcc cacttgtacg tcgagccgtt
    16501 cgccggtgga ttcagtgtgg ggctgagcgt ggcccaggac tatcccgaca tcgaaatctg
    16561 gctaaacgac ctagaccaag acgtatcggc attctggcac gtgctggcca gtggctctga
    16621 cagggagtgt cagcggttac ttaaccttat catccaagct ccatctgtag aactgcttga
    16681 gcaactccgc gccaccccac ccaaggacca ggttgagcgt gcttaccgtg ccattttctt
    16741 taaccattgc acgttcagtg ggatggcaaa ttctggccct cttggcggat atggacagga
    16801 tggcaagtgc catatcggtg acaggtttaa ttgcagaaga atcatcgccg aatgtatcga
    16861 ggcccgcaaa ctgctccgtg gtcgcaccaa ggtcttttgc caggacggga tacagcttat
    16921 ccgtgaagct cctgtcgatg ctgtcctgtt cgtcgatccc ccctatgtca aagcaggcaa
    16981 atccgtgtat cgggaatatt tccagaccga gcagcacaaa gcccttgccg aggctctccg
    17041 tggacggacc agatgggtgg cgacgtatga catggctcca gaaatcaagg atcactacga
    17101 ctgggccatc ctggaagaac tgccggtaaa ctactcgata aagtcattta acacgggtct
    17161 gactcgatcc accgaatatc tcatcacccc gcccgcgcct gtgccggaaa cagcctcgga
    17221 agtggtgtat tcgtcatcga gttcgagcga atcccttgat gagacggcac atgcgggtaa
    17281 ggcaaaaaga caggaagaga aagatcttgg ccgggaccat cacccggccg tatccctggc
    17341 caagagtacg gtcaattttg cggattacga gtacctaacg acgaaggaag cagccatgta
    17401 tctacggagg agtccatcgt ggctcctgca taacaaggaa atcccttacc tgacgggctc
    17461 tccgaatacc tacaaaaagt cggacttgga tgcatggttc aacaagacca agagcggaga
    17521 gatctgacca tgagtgtatg gcaaagaaaa gaccatttgg ataaatggtc tcatcccgtt
    17581 tggtactact ctttttggca tagggggcaa cggtatcgta agggaggatt tctaaccaag
    17641 ggccgcgcga gtgctgctga agccaaacga aagcacgatg taattattga agggaaggtg
    17701 tttcgcggca aagggaacaa gtcattggcc gagattttca gcgagtactt ggaataccgt
    17761 gcatgcgact gtgccgcgtc cacaatagcc ggtgaaaaga cgcggaggcc ccctctcctg
    17821 ggctttttca acaagacccc catgacatcc atcgcagtat cggatgttca aaaatacgtg
    17881 gaggatcgga aaagaaaagg catggccaat agaaccgtaa accttgaact catcaccctt
    17941 cgcaacctgt tcaagtttgc gatgcagaga gagtacgctt ttcagaatcc ggcgaaagaa
    18001 gtcaaaaacc tgaaggaagt gcttcgtgat cgcccggttc ccaaccccga gcagatgagg
    18061 gcattgcttg aagccggaaa agcagaaggc tcccaagtct attgctggat ggccttgatg
    18121 gcgtacacag ggatgcgacc cagcgaatcc ttccacctct cctgggacga tatcgacttc
    18181 aagaggatgc agatccttgt gcgtcccaaa caagagcacg atctcaagac tggacgattc
    18241 agggcggtca acatcttgcc ggaactcctc cccatcctat tggcgtggca aacggaatgg
    18301 gagcaggtct ttgccgacgg gaagccccac aactgggtgt tcttccatcc gcgccgaccg
    18361 gcattccggg ctcatggcta tccgaagcac ttcaagagag ccgttaagaa cgcggggttg
    18421 aaaggattca cgccttatac tttccgccat tatttttgca gcagagccat tgaaagtggg
    18481 tcaaatatcg aggcggttaa aatagttgtc gggcataccc cctcgtccac ggtgctccaa
    18541 aaacactacg cacacctctg ccagccgttc atcgatgccg aactcgccaa ggtaaagatc
    18601 gtgtaggttc cctgccgtct ggcccgagca cgtccccaag tccatggccc caccgccgaa
    18661 agccgtaccg cgcctttatt acggccttca ggtccaaagg ggcgtccaag cagcttctgg
    18721 aggggaaaat ggctaggata gcttcctggg cgagaaaaac gcctattgga gcccctccag
    18781 ccaaggcacc acctcctttg gaaggagtgt tgaaaattcc atggcggcgg gactggtaga
    18841 gccgccactg cccctacccc tgctcacggc tgcaaagaac cttttggccc caaattcggc
    18901 cacatggctt tttgacaaag aaacagccct gccggcggga cacggcttca aggggctcaa
    18961 cagcaagaaa agccctgtta aataaggctg aaatgagtgg cgggaaaaat gaagctggtg
    19021 gccagagtcg ggatcgaacc gacgacacac ggattttcag tccgttgctc taccaactga
    19081 gctatctggc cccgtgcgag aagggcatac taggcttttt ccgctcgatg tcaacgctcc
    19141 gggctcctat gctcgcggca tgaattcctg cccgttttgc gcggtggcgc cggagcggat
    19201 cacggccgag gacggcccgt gcctcgcgct ctccgaccgc tacccggtgt cacgcgggca
    19261 ccagttgatc gttccgcgcc gccatgtcgc ttccttccgg gagttgacgg acgaggaatg
    19321 gcaggccgtg ctccgtctcg cccgccggct cgcggagcag ttgcaggaaa gcgatcccgc
    19381 aattcagggt ttcaacctcg ggatcaacga cggggccgcg gcgggtcagt ccgtcttcca
    19441 cgtacacgca cacctgatcc cgcgccgctc gggcgacgtc cgccggccgc gcggcggcgt
    19501 ccgcggcgtg atcctcggca agcaggatta tcccggcgat tccgcgcctg ctccaacagg
    19561 ctgatccccc cggcgccccc ggccgttgca tcgcgattcc aaaatggatt aaccttatgc
    19621 gcatatgcgc ataaggttga aagcctctgc cggcaaacca accgggatgc gagcggcggc
    19681 ttcagcgccg ggaagacagg aaccggccgg ggcggacgtt ggtgggcttg ccgccggaca
    19741 ggactgcctg cccgttgacc atgagcgtct cgatgccgcg cgccaatcga tgcggctcct
    19801 cgtaggtcgc cgcgtccccc accccgcccg catcgaacac gacaatgtcg gctttcatcc
    19861 ccggctcgat gaggccgcga tccttcaagc cgaattgccg cgcgggcagg gacgtcatct
    19921 tgcggaccgc ctcggccggc gacacggtcc ggccatccag cgaggcccgc aggaagcggg
    19981 gaaaagcgcc gtacgcgcgg ggatgcggat gatcgcggct caacgggccg gacggcgcac
    20041 gcagggaggc gtccgacccg atcatgacgt acggctcggc gaggatgcgc cacatgttgt
    20101 cctcgctcat gccgaagaaa atgccggagg tgtgcaactc gtccgtgtcc atcaggtgca
    20161 gggcggcatc caccggctcc aggcccagcc gccgcgccac ctcgacgatg ggccggccct
    20221 tgaacccggc gttgcccggg tggcgcacag aaccgatgac caggttttcc cagtaggccg
    20281 cgtcgcgccg ggctgcgatc tcttcccgga tgcgcgcgcg ggtcgccggg tcgcgcagtc
    20341 gcgtcagcac ttcctcgcgc ccgccgccgg cagcccaatc gggcaggatg acgtccaggt
    20401 ccgtgcagga cgcggtgtag ggatatcggt ccgcggcgac ctccagccct tccgcccgcg
    20461 cggcccgcac ccggtccagc gcggcctccc gcagccccca gttcgcccgc ccggaggtct
    20521 tcaggtgcga gacctggacc cgcacgccgg tctcgcggcc cacgccgatc gtctcgtcga
    20581 gggcctcgag cagtcgcccg ccctcgctcc gcatgtgcga cgtgtaaatg cccttccgcc
    20641 cggccaccac ccgggccaat tcaacgatct ccgagggcgc ggcgaacatc ccgggagagt
    20701 agatcagccc ggtgctccat cccgccccgc cctcgtccat gcaccgggcc agcagcgcct
    20761 tcatctccgc caattcatcc gccgttgagc gacgctcctc gtaccccatg accgaggccc
    20821 gcaacgcgtt atgcccgatc agcaggacca cgttgaccgc gggcttcacc tgctccagca
    20881 gggcccggta ttccgccacg gtgcgccacg aaccgggata cggcatcgcg cgccagtcgg
    20941 acggcatgag gtagcggccc aggcgcggcg cggcggacgc gccgcagttg ccgacgacct
    21001 cggtggtcac gccctggaac accttgctcg gcgcagcggg ctcgatgagc aggtaggcgt
    21061 cggaatggga atgcacgtcg atgaacccgg ggcagacgca acgccccgcc gcgtccacga
    21121 cgacgcccgc ctcggcgagg gacaggtctt cgaccgcctc gatacgatcg cccagcaggc
    21181 cgatgtccgc gcgtcgcgca ggcgtcccgg ttccgtcgag cacctgcccg ccgcggatca
    21241 acacgtcgaa tctcacggcc cgcctccggc gcggcgctgt tcgatcttct cgatataacg
    21301 gcgcgcctcg ggactgccga cacggagaaa ctccgcttcc gcctcttcca accgcccggc
    21361 gtcgaacagc gccctaccga ggttgaagcg cgcccgcccc ttgccgggaa atgccgccac
    21421 cactttccgc agaggatccg cggcttctgc cgggcggccg agccggcaca acgtcacccc
    21481 gagggcattg ttcaattccg gatcgtcggg gcgcgccgcc agggcggcct gcaaacgctc
    21541 cagggcctcc ccggcgcggc ccaggtcggc gagggtcatc gcccattggg caagggcctg
    21601 cgggttgccg ggcatcctgg ccagggcgct ttcaaaatgc gcgcgggcct cttcgttccg
    21661 gccgaggctc gcgcagacca taccgagatt gacctccaac tgggggctat ccggtttcag
    21721 gcgcaacgcc tccctcaagt gctcctcgga ctcctcgtag cggccgagtc ccgccagcag
    21781 cgtgccgagg ttgttgtggg cattcatgta gtccggcttg agccggagcg cctgccggta
    21841 ttgctccgcc gcttccgcgg gccggccggc ctcgcgcagc gcgttgccgt agtcgttgtg
    21901 tggctccggg ctgccgggca ccttccggac cgtgtccgcc cacaggacaa ggggatcccg
    21961 gtagtccgcg ttgcgccggt gcgccgccca cccgaacccc gcggccagga tcagccccgc
    22021 cgccaggtag agggagagat cctccttccg cctcgtgcga accgccgcca ggaacgcgcc
    22081 ggcggagacc agcgcgacca gggcggccag cggcgcgtac atgcggtgct ccgccaccgg
    22141 ctggccgagg atcgggacga acgtcgagga cggcgccagc accccgaaga agaacgcccc
    22201 ggcgaacccg gcccgcggat ggcgggccac cgcccacaag gtcgcgcccg ccagcgcggc
    22261 caggagaagg gcttgcggca ggacggcgag cggattatcg atcaggtcca tgccgtagaa
    22321 aaagaccagc ggctgcggcc agaacgccag ccggatcatg cgcgccacgc cccagcactg
    22381 ggtcaggaga taatgccacc gcatgcccgg ggccagcagt tccggcgtca cgtggggcga
    22441 gtaggacagg tggcgcgaca ccggccagag cgccgtcagc cacaacatcc cgtgcagcca
    22501 gccgcgcagg cggaggacat cgcgccacga tgccgccagg aaggcccggt ccaggagcag
    22561 cgccaggatc ggggcgacga gcatggtttc cttgctgccc atccccagca ggcagccgaa
    22621 cgcggcaacc gcggaccacg cgacatcatg ccccgcaacg gaatccgccg accggcacag
    22681 ggcatagagc gtcacgaaga ggaacaacgt catgagcagt tccgctcgct ggctcaagta
    22741 attcatcgcg gcggtattga gcggatggac caaccagagc aacgtcgcaa caaaggccgc
    22801 gagttgggcc ccgtccagga ataccgcacg caggcttccg ggcaaccgct ccggatcggt
    22861 ccagggcgac cccgcggcgc gcctctcccc gccccgcagc agccggatca gcaacaggta
    22921 gagcgcccag ccggcgagca ggtgcagggt caggttggtc cggcgatacc cggcgaggtt
    22981 ttcccccgat ttcgcgtagt cccaggccag cgagacatta acgatgggcc gcgtccagaa
    23041 gctgatggga cccttgggcg gatgcaacgc ggcgctcaag ggccagagcc ggcggatgcc
    23101 ctcgttccga atgatgctgt tcaggtcatc cagaatcaag gtgccgtgga aactgttgct
    23161 atagacgaga tagccggcca gcatcaacag gagcgcaggg agccagaatc cgcgccgttt
    23221 cgaaaacggg cccgaggtga tccccggccc gcccggtggc gcgatcggtt ctgtatgttc
    23281 agtagccatg tccttcctgc ggcctcttct ccggcggaag gcccacacct ggagttatgc
    23341 cggggcctcc gctcatcggc ccgcggaacc gattcaagga ttcccggatc tccgcctcgg
    23401 catggaggag tgccgggtga cccggatgga ccttcagggt gtcccggaga cgacccaggg
    23461 cctcctcgcc gcgccccatt tccttcatcg tctgcgccca ttgtaccgta accagcggct
    23521 caaacggaac cgctttcagc acctgatcga gttgctccgc ggatttcgcg aactcgcccg
    23581 cctcgaggta cgcggacgcc aggttctggc gcgccagggg attatccggc ttgagctgga
    23641 gggcctgttc cagccgcgcg atcgcctcgg ccgcgcgatg gcccatcgcg aggagggcgc
    23701 cctcgttgac caacgcgtcc acgtagtcgg ggctcaactg cacggcctcg cgatactcgg
    23761 ccacggcttc gtcaaggcgc ccggcttccc gcagcgcgtt ggcgtaatca taatgggcct
    23821 ccgggacgag cggcaccttg cggacggtgt cgcgccagag aattaacgga tcgcgatagt
    23881 cggcattgcg gcaacgggtc accgccccga ggccgatcgc gccgatcaac gccaggccca
    23941 gcagggttcc cgccgcgcga cgggggccga ccatcctgct caacacgacc tgtaacgcac
    24001 ccacccccag cgccagcagc gcggccaacg gggcatacat ccggtgctcc gccaccggtt
    24061 gcccgatgat cggcaccagg gtggatgacg gcgccaggac caggaaggaa aaggcgcccg
    24121 cgaatcccgc acgcggccgc cggaccagcg cccaggccgt ggccgcggcc agcatcccga
    24181 gcagcagggc ctgcagccaa acggcagaca gatcggtggc caaatcccgg ccgtagtaga
    24241 acaccagggg actcggccat aaggccagcc ggatcatgcg aaccaagccg cggcactggg
    24301 tgagcaagta gcgccagcgc gtctcttccg ccagggcggc ctccgtgatg tgcggggagt
    24361 acatacactg gcgatggatc ggccaccacg cggtcagcca gagcgtcgcg tgcatcaggc
    24421 cgcgctgccg ccacagcgcc cgccaggaat cggcgagaaa aagccggtca tacaacaggg
    24481 ccagcagcgg ggctgccagc atgtgttcct tgctgcccat gccgagcagg cagcaggcgg
    24541 tggcggcggc ctgccaccac cgggcccgcc atcccgcccc atcggctgcg cgtcccacac
    24601 aatagagaag cgtccacagg cagagggcaa cgaggatttc gccccgctgg ctaaggtaat
    24661 tcacggccgc ggtgttgagg ggatgcacca gccacagcaa cgcgattatc caagccagcc
    24721 gatctgcgcc gtcggagaag aaccctcgcc aaccggccgg caacgggcat gcgccctctc
    24781 cgcttccctt ctcggggccg accggacgga gacggagcgt acgggccaac aggaaataca
    24841 gcgcgcatcc ggagagcact tgcagcagca ggtttgtgcg gcgatagcct gaccagtccg
    24901 tccccgagac cgcataatcc aatgcaaagg tggcgtttag gatgggccgc gtgtagaaac
    24961 tgatagtgcc ggttggagga ttcagcacct cgctcggagg ccagagtttc cgaatccctt
    25021 cgttgtcgcg aatgctcttg aggtcgtcga gatggagcac ccggtgaaag ctgttgccgt
    25081 aggccaggag ccccgccgcc acgagcagaa ccgtcgggag cagccccttt ctggaccgtg
    25141 aaccgaccaa accggtcgtc tcaggatcca agtggcccgt caccgcggga ttcatcgaag
    25201 gacggcttct cctttttgtc cgaggccttt tcagggaccg gaccaccgag cagtgcgatg
    25261 acctcctgct gaacctgctc cagttcgtcg agtcgcaggc cggggtcggg cacgacaaag
    25321 acttcgcctg ttttccgatg ctcgtaaaac cgcaccttga gcccgtcctc gcgggttttc
    25381 acgtcgcgtt ccacgaggag gcggcgccgc tccagcatga cggccaggat gaaaatcgcg
    25441 ctccgtctgg acggatcctc ggtttccatg aactggcgga ggagggattc cgccgtctcg
    25501 cgcttgatcg gttccggggg cgggggcgcg ggcatgtggt agacgctttt ccacgcgctc
    25561 accgaatcgc ccttctgcgc ccccggccag cagacggcgc agcaatcctg gcgctcgtag
    25621 ccttccgcgc cgaacgtcag ccgggaccat atcggctcct tgtccgcgaa ggcgcggtga
    25681 caggccttgc actcggcgca gggctccttg atgttccatt cttgttgatt catttcaccc
    25741 gtccgtccgc atcgctccgg gctggaccca gtttcaagtg tgtccagcgg tcacgcggca
    25801 agcccgccgc gagcatcgtc atattcaaat gatacgcaac attttacacc aatacaattg
    25861 tgacacactt tataaaacat gtcaatgggc tttcccctca tggaatcgtc gggggcgcct
    25921 gctttcccgc cagttccttc gcccattcgc tgtccgggta gcgcgccgtt aattcgtcca
    25981 cggccttttg ccggtcttcg ccgcggccca ggcgatcgaa ggcggccgcc gcctcgtgca
    26041 aactcctggg cgccagttcg ggatcatcat acagcaccgc gacgctcatg tagaatcggg
    26101 cggcgtcctc ccagttttcc ctcgccgccg ccgcgcggcc cagcccgaag tagctgcgcg
    26161 cgcggaggga catggcctcc ggctccgtcg cgcgctcggc cgcggcctga taagccgtct
    26221 ccgcgagcgc cgcgtccccc gtggccaagc gcaaatcccc gagccgcaac gccgattccg
    26281 ctgcttcgag cgtccgggct tcctggtcca gcgcccgggc ataggcctcg agggcgcgat
    26341 cctgctcgcc cgcggcctcg cggctgcggc cgacccagta ccagccgatc tgcttccact
    26401 ccggcgtgcc ggcctggtcc gccagcgcct gggccgcctt caaagcgtcc gccggggcct
    26461 cgtgtcgcag ttgaagatcc gcgagccatt cgagcatcgc gggcggcatg tcgcggcgga
    26521 tcggcgtgtc cagcagcgcc tgcagcagcg ccgccgattc gtcctcgcgg ccctgcttct
    26581 gcagcagcgt cgccagccgg tacttgagcc tcggcagctc ggcctccgcc ggcttccgag
    26641 ccagcgcgtc gcgcacggcc tcctcggcct tgtccagctt gccggcctgc tcgtgcagga
    26701 tcgacagcgc caccagcgcg tccaccgcgc gttcggtctt cgagtagtcc ttcaatagtc
    26761 tctcgagcgt ccgccgcgcg tcggattcct tcccgagccg gatctgcgcg acggcgacct
    26821 ggtagagcgc gacgtcggac tggcgatagt ccgggtgctt ctccagcagc gcggtccagt
    26881 cgcgcagcgc ctcgtcatgc cggtcgagac gcgcatggca gtaccccgcg gtgaagaggg
    26941 cgtccgggac cagcggatcc cgggagtatt tctccgcgat cttccggcag gcttccgccg
    27001 cggccgcgaa atcctccgcc gcctgctgga gttccgcctg gcggtacagc gcctgcgccg
    27061 cgcgatcgct ttcggggaac ttctccgcca gcgcctggaa ttgctccagc gcctcggcgg
    27121 accggttcag cgcagcgtag gattcggcca gcagccagcc gcggtcctcc tcgaccgccg
    27181 cggacggcgc gaggccggcc agttgttcga cgactttctc gtgctgcccg cgccggaacg
    27241 cgatgagcgc cttctcgtac gcggcggccg gccggcgggg cccggacgga aactgcatca
    27301 tgagttcatc gtacgccgcc agggcgaggt cggtgcgctg caactgccgg agggcattgg
    27361 ccttgatgta cagccagtcc tcccgcccgg cctcttctcc cgccgccagg cgctcctcgg
    27421 ccaacttcag cgcggccgcg ttcttgccgg actgcaggaa ggcccaggcg atctggagcc
    27481 gggcctccgg gacgcgccgg tcgccgggat acttttccag gagctgctcg taggccttcg
    27541 cgctctcttc gaaatcgccc gccttgaacg cgtgctcggc ccactggaaa agggcctcgg
    27601 cgccggcgcg cggcgtcgcg ggttgctccg cggcccggcg atacaggtcg cggacctccg
    27661 cggccggctt cccggtccgc tgccccaggt cggccagggc ctggcaggcg tacccgtaga
    27721 acggcgattt cggatgccgg tcgatcacgg tctgcagatg ctgctccgcc tcgccggcct
    27781 ggcccagctt gtcgaggctc gtgccgagga agtacgccgc cgaggcggcc acgtcgtccg
    27841 gcggcccggc gtccaggagc gcccgcaaca ggttcgcgcc gtcgaggtac tgaccggcgg
    27901 tcacgaagag ttccgcgcgc cggaactcgg cgaggaaccg gtgcgggctc tgcgggaaat
    27961 ccgtgaggat ccgcttgtag taccgctcgg ccatttcccg ccggcccagt tggcgatggc
    28021 actcgccggc gcggaaaagc gcggcgtcga tcgccacgcc gggcggctcg tcgcggatga
    28081 cgagcaggta ctccttcacc gcgaggtcgt acatgccccg cacgtagagc ccgtccgcga
    28141 actccatgcg gtcggaaggc ggcgcgttgg tctgcgcccc ggccgcgaga gcggcgaggc
    28201 aaccgcaaca catcagaaac ttcgcggcaa aacgcatggt ccgtcagggc gccgccgggc
    28261 tctccggcag gctggtggcg aaggagatgt tccagatgtc ggccttgcgg cagaggtcca
    28321 gcacgcgcac gacgtgctcg taggcgacgg ccttgtccgc ccgcaacacg accggctggc
    28381 cggggaacag cgaggccagc cgcttcagca tcgcgccgag cgcggcatcg tcgagggcct
    28441 ggttgttcac catcgcgccg ccgtcgggcc ggatgttcac gatgatctcg ccgggcaggc
    28501 gctgcggggt ctggccggtc tcggcggtgg gcagcgtgat gtcgatctcc gtctcccact
    28561 gcgcgaagat ctgcgtggtg atgaaaaagg acaggagcag gaagatcacg tccatcatgg
    28621 gcgcgagcgg aaacgcgccg ggctcctgcc ggatcgcggg gcggaaattc atgacgcgcg
    28681 cccgaggagg tgggtcagca ggtcggtgga cgccatttcg aggcgggcga cgacggccga
    28741 ggaccggttg cggaagtagg cgtagaagat catcgccggg atcgcgacga ccagcccggc
    28801 caccgtggtg atcagggcca gcgagacgcc ggcggcgagc agcatgggct tggccttggc
    28861 caggtcgagg gcgatggcgt tgaaggcctg gagcatgccg accaccgtgc cgagcagccc
    28921 gatcatggga gcgatgaccg cgatatccat caggtattgc acctggttct ggatgcgggt
    28981 cgcctggcgg atgccctcgc ccaccatgat ctccttgagc atgtcgggct ggacgggttt
    29041 cgcctccgcg acataatcca gggcggcggc ggtcacggcg gccaggggag agggccggcc
    29101 gtcgcagagc ccgcgggcct cgtccgcgtg accctgccgg agcagttcaa aaaccttctg
    29161 cagccagcgg cgcggggcca cggcctccgc gcgcaggacg atcaggaggt acaggatgaa
    29221 agccacggcg agcacggaaa gggccgcgat cggatacatc agccagccgc cggccttgaa
    29281 gatctccacc aggttcatgc cctgcccgga acccttcggc gcagcctccc ccgcctgggc
    29341 ccaggccggc ccggccgcca ccgccaaccc aaccgcccac ccgcccgcgc gcttccacat
    29401 agacacgtcc tctctttcgg aattgaccgc ccggccagtg taaaaaggcg ggccggattg
    29461 tcaagaaaga ggggcggcgg cgcttactcg aagcggcgga cgtagccgag ttccgtcagc
    29521 ttcctctgcc ccgcgcgcga ccggatgtag tcgatgaacg ccttggcggc cttcgattcg
    29581 cggctccgga tcgtgtagag atgcagctgg cgggcgtacg ggtaggtccc gtcgttgatc
    29641 gtctccacgc tcggtcggat cttgttgatg ctgacgacgc ggattccgtc ctgcttcgcg
    29701 acggccatgt ccacgtagcc cacaccggcc gggtcggcgg ccacggcccg ggcgatggcc
    29761 gcgtagtggt cgaacatctt cgcgtccttg tcgtacggct tgtcggccat ggccagctcg
    29821 cggaacccga ggtgcgtgcc ggactcggga tcgcggatgt acaggttcac cggcgcgtcg
    29881 ggcccgccga tctccttcca gttcttcgtc cggccggtga acaggctgcg cacctgtcgc
    29941 gccgtcaggc ccttcagcgg gctgtccgcg tggacgatca cggccacgcc gtagtagccg
    30001 atcgtgtagt tccggaggtc cagcttgcgt ttcgcgacca ggcgccgctc gtccgcgttc
    30061 acggagcgcg aggacgacgc gatgtcgcat tcgccggcga acagcgcggc gaagcccgaa
    30121 ccgctgcccc tggactccag cgcgatctcg acgtccggat ggctcttccg gaactcggcg
    30181 atcagcgccg ggcccagttc ctcgccgaac gtattggagc ccttgatgga gaccttcttc
    30241 ggttccgccg ccgcggcgcc acccgccagc gccagcacgc atcccagagc cagcatccct
    30301 cttttcacga tccacgctcc tttcgtcccc taccccgcca cgatgtgccg ccgccgctgc
    30361 ttgcgcgtga ccagcagcat cgcgatgccg cccagcacgc cgaagcccgc catgatcgcg
    30421 taccagaacg tgtagccgta ctgctccttc gtggcgtcca gcacgcgccc cgtgatggcc
    30481 agcgaaatcg ccccgccgta atactggaac gagtcgatca cgcccgccgc gaacccggcc
    30541 atcttcttgc ccccgatgtc catcggcgcg gccgacccca cgaccgagtg cgtcgagttg
    30601 gccgtgaagg cgatcagcac gaggaagagg cagcccagga agatgccgcc cggcgtcggc
    30661 ccgaccgccc cgctcaacag caccagcgcc gccacgctga tgacgatcgc ctccaggaaa
    30721 tagagcccca tcgccaccgg cgaccggtgg cccttgaaga ccttgtccga cacccagccc
    30781 gagaggaacg agcccagcac ggcgaccatg ggcatgagcg acagcgtgag ccgcgccgcg
    30841 ttcgggatct ccgtcttcat gtcgaagccc agctcgtcct ggaagtacag gatcgcgagc
    30901 tggtcggaac tcgtgcggac cgcccccgtg cacgcgtagg ccatggcgta gtaccagacc
    30961 agcgggtgcg tgaagatcgt cttgaaggat tccttgaggc tgaccgtcac ccccgcggaa
    31021 tcgtcgagct cgtccttgat gacgccggga taaccggcct cgtccggcgt cggcttggcg
    31081 gccagggcca tgaagacggc gcaggcggcg gtgaacagcg gcggaatgcg gaacagccag
    31141 cgccactcgc cctcgcccac caccaacacc ccgaaggtga agccaaggag gatgaccggc
    31201 gccaactggc tgatggccac ctggcccagt tgaatcatga acccgaagat gccggcaaac
    31261 gtcccgcgct cgcgccgggc gaaccaggcc gcgttgatct tgaccatgcc cggcgcgccg
    31321 aaggactgca tgtagccgtt gatcagccag atcagcgaga aggtggcgaa cgagccgacg
    31381 aacgagccga acccgaagaa aaggttcagc aggatcgtgc ccacggcgcc gatcagcatg
    31441 gaccgcttgc ccccgatgcg gtcgcacaac agcccgttga tcaactggcc ggtcccgtag
    31501 gccagcgacc agatggccag catgtcgccg atttgcgtct tggtgaagct gaactcctcc
    31561 accatgcccg gggtggcgaa ccggaagttg taccggcaca tgtagtagga agcgtacagc
    31621 aggcccagcg aaacccagtt caggcccctt cgcgcgcgaa agccctccgg atgctgcagt
    31681 ctcccgccgc tctcctggcc ggtcgagttg tccatgggct gttctcctgg ccggtcttcc
    31741 gccccggcat ggcctgatgt atacgccgtg agcggctcca gttaaagccc gttcttggga
    31801 tccagccgct ccgccagccg ccgcgcggcg atctccgctc cgccccccgg caattcgtcc
    31861 gcgggctccg gctcgcgaac gatcgcgtcc agcgcctcct ccaggtcgcc cgcgtacagc
    31921 cggtccgccg ggagcagccg gtgtttcagg aaccgcttca cgccggcctc gagaatggga
    31981 tgctcgcgga aatccgcgcg gtcgacgtag accagcggct tgcggttgac cacgcactcc
    32041 gaaaccaggc cgaaccccgg cttggaaacc acgaggtccg ccgaggccag cacgtccgaa
    32101 aacggcatcc gttcccggtc cacggcgtgc acgttgcggc gcgaccattc cagcggccgg
    32161 acggtgaaga actcgtacgc gccgagccgc tccacgcggt ccagcgcggc cccgtcccac
    32221 tccagcgtgg tgaaggaaag cagcacccag gtccggtccg ggcgcgcgcc ggtcagcgcc
    32281 gccagctcct cccgccgcgc gcggccgggc gcggcgacga gcgggatatc ctcgatccgc
    32341 cggaacgcgg ccatgggctc gtggaacggg agccgcaaca gcacatcggc ccgggcatac
    32401 ccctcccgga acgccgccgc ggcttccgcc cagcgcgggt cgcgagggat gaactcctcg
    32461 tagatccagt tccagccaaa gttgccgacc gccacggccg ggatcccggc gcgccgggcg
    32521 gcctcgagcg gcatcgcggg aatatccgcg acgaccagcc cgacctcccg ctcgcggagg
    32581 aaccgggcct cccgcccgac gagttcctcc cgccgcccgc acagggcccg gacgcgatcc
    32641 agcgtcgcgg gcacgtccac gcgcacggag tcgagctgga ccatcccgac gtcgaacgag
    32701 cccggccgga tgtccgccgg cgcgcccgcc agccggttgc gcaggaacgg cccgggcagg
    32761 tcgctgacga tgacgacggg aatgccggga tagccgcgga tgaaggcgcg caggatgtcg
    32821 gacgaccgca ccccgtggcc gtagccgtgg gcgctgatat aatacgcaag gccgggtttc
    32881 atggccgccc ccccagcata atgctttcgc gcgacggaac aagcgccgga gcggcttggc
    32941 ttccggcgcg ggaccggcta tactgcgggc ggtaaccggg gcatgctcac aagggaggcg
    33001 aaccatgtcc aaggaaaaga aaggcggcca ggatcgcggc ggggacgaag agaaggatga
    33061 cgcgccgcac gggaagctga agcggaaggc ctacgagaag gaactcggca agctccaggt
    33121 cgagctggtc aagctccagg agtggatcaa gtacaagggg ctccgcgtcg tcgtgctctt
    33181 cgagggccgc gacgccgccg gcaagggcgg cgcgatcaag cggatcaccg agtcgctgaa
    33241 cccccgcatc tgccgggtcg tcgccctcgg cacgcccacg gagcgcgaga agacgcagtg
    33301 gtacttccag cgctacgtcg cccacctgcc ggccgccggc gagatggtcc tcttcgaccg
    33361 cagctggtac aaccgcgccg gcgtcgagcg cgtgatgggg ttctgcacgg agacggagta
    33421 cgaggaattc ctccggtcct gcccggagtt cgagcgcatg ctcatccgct cgggcctcat
    33481 cctgatcaag tactggttct ccgtcagcga cgaggagcag gagcgccggt tccaggaccg
    33541 catccacgac tggaccaagc gctggaagtt gagcccgatg gacctgcagt cccgcgcgcg
    33601 ctgggtggac tactcgaagg ccaaggacga gatgttcaag tacaccgaca tcaagcaggc
    33661 gccctggtac gtggtcgagg ccgacgacaa gcggcgggcc cggctcaact gcatccacca
    33721 cctgctctcg atcatcccct acaaggacct gacgccggtg aagctcaagc ttccgcgccg
    33781 ccagccggac accggctacg agcggccgcc gatcaccgac cagaccttcg tgccggaagt
    33841 ctactgagcc cgcgtcacgc ccagcggccg tagccgcccg gattccgggc gctccacagc
    33901 cgcgcgcggc ggtcgcggaa ggccgcgagc cgggccggat tgcagaacgg gcgcaccagc
    33961 agcgcgccgg ccgcgaaccc gccgacgtgc gcccagaacg ccacgccgcc gtgccccgtg
    34021 tcgaacgcgc cggcgaccag ctgcagcagg aaccagtagc ccagcatgaa gaacgccggg
    34081 accaccacgc ggttggcgta gaaaccgatc cagaccagca ggtgcacggg cgcatgcggg
    34141 tacaacacca ggtaggcgcc cattactccg ccgatcgccc ccgacgcgcc caccatcggc
    34201 accaggctcg acgggcccga cagcatctgc gccgccgccg cggcgagccc gcagaggagg
    34261 tagaagacca cgaaccggac cggccccatg gcgtcctcga cgttgtcgcc gaagaccagg
    34321 aggaaccaca tgttgccgat gatgtgcatc cagccgccgt gcatgaacat ggaggtcagc
    34381 acgctccacc agttcggcgc gccgtcgagc accgcgacca ggcccggccc cgccggcacc
    34441 tcggtccccg gccgcacccg gcccagcagc tcgccgggga tcaggccgaa ccgccatacc
    34501 gattcgctca agccggggtc catgcccagc cgctgcacga agatccacac cagcgcgttg
    34561 acggcgacaa tcgccatcgt ggccaccgat gcgcgcagcg tgggattgtc gtcgcggagc
    34621 ggaaacatgg ccctacggta gcgcgctgat ccttcgcgct caagggcgaa gcggatttag
    34681 tgggcaaccg gccggcgaac gggcatactg aatacaagcc agcgggcatc cgtcgattca
    34741 tgggtatgtt caccattcca ggccagcctc ggtcccggcg aacggcgctc ccgccctttt
    34801 tccgcctgtg gctgctcttt tcctgtcaac cgggcgcggc ccccggccgt atggaaaagc
    34861 aggacggtga aggcgccgcc atggaaccga cggacgagac gctgatgcaa gcttacgggc
    34921 aaggcgacga aacggccctg gcgcaactgg tgcgccggca ttccggcccg ctgctgggct
    34981 acctgacccg catgagctcg aatccccacc aggccgagga tctcttccag gaaactttcg
    35041 tgcgcgtcca ccgccacgca cagaacttcc ggcccgacgg ccgcttcaag ccgtggctct
    35101 acaccatcgc cacccgcctg gccgtggacg aactgcgacg ccggaagcgc acgcccgtcc
    35161 tgctctccat ggacgaacaa tccgaggaac atcactccct cgccgaccgc attgccgacc
    35221 cggccgccgg ccccgccgac tgcgccgcgg ccgccgacct ccgcgcgcgc gtgcgcggcg
    35281 ccgtggagcg gttaagcccg gcccagcggg cgatcctgtc gctgacctac ttcgagggct
    35341 tcacctaccc cgaggcggcc cgcgccctgg gccgctcgct cggcacgatc aagaaacaga
    35401 tgtcccgcgc gctccggtcc ctggcgcgcg cgctgcccga cctggcgccg ggccacgccg
    35461 cgggaggcgc ctcatgaacc atcacctgaa agaacagcag atcctggact acctcttcgg
    35521 gctggcggaa gaagcggaaa gcgcccggac cgagcagcac ctggccggct gcgccgaatg
    35581 ccggaagcac gcggacctcc tgcggcgccg gttcgccgcg ctggagtctc ttcgggaaac
    35641 cgaggccgca tccgagcggc tgatcgccga caccctgcgc cgcgtgcggg tcgaccaccc
    35701 cgtgagtgag tcgcgattcc cctcgttcgc gtggatcgcc gccgcggccg ccgccctgct
    35761 ggtggccgtg ttcatcgtgg cgccgcgcat cggacgggaa ccggccatgg aactgacgca
    35821 aaccgaacta tcgcctgccc ccgcgggcgg cggcgtcgcg gggattccgc cggcgtccgt
    35881 tgcggaaccc gccgccgcgc cgcccccggc cgcggtcccg ccgccggttg agccgccgac
    35941 cggcatgctg ctggcggata gcgccccgat gatcgcgggc gaagtcgcgg accgcgacaa
    36001 ggctctggcc gaggcggacg agatcctttc tgatgcggcg ggccaacccg ccgcgccgat
    36061 gctcgcggcg gcccgggcct tcgcgccgga gaggtcggcc ggggcgggaa tcagcgaacc
    36121 gcccgcgccc aagaccgacg cgacaaccgt ggcgaaaaag accgtccatc gcccgcgcaa
    36181 gccgcggccc gccgacgcga agccgtacac cgcgcggctg ccccatggat ggagcttcgc
    36241 gccgaccaac gaggcgcggg tgatgctgat cccgctcaag agcaaggcgg acgggctcat
    36301 gccgccgccg gccgagggct tccgcacccg gcactggcag gtccgcgtcg tgaacgacgc
    36361 cacaaacgcg cagctcgttt cggtgacccg gaccttcgac cagccgggtt gggacctgca
    36421 actgttgaat gacgaaggcc gcctggcccg ccggtcggac ctggaggccg tgatcgaagc
    36481 cgacgtcccc gcgggcggcg tccgggccgt ccactgcacg atttcggaac ccctgaagga
    36541 gaaccagcca tgaagagcct gctttgttcc ggtttggtga tgtgtctgtg cgccgcatca
    36601 gccgcggcga agcccgaggc tcctacgcag ggggcattga gcacggtcat caaggacgac
    36661 aacggcaagg agacaatcat cgacctgccg ctgaagcaca cggaggtcga ggcggagatc
    36721 agcgggtatg tcgcgcgcgt gaaggtgaag cagacgttca ccaacccgtg cgaccagccg
    36781 atcgaggccg tctacgtctt cccgctcccg cagaacgccg ccgtcaacga catgcagatc
    36841 cgggtcggcg accgggtcat ccgcggcctg atcaagaagc acgaggaggc caggcagatt
    36901 tacgagaagg ccaagcagga agggaagacc gcggcgctgc tcgagcagga gcggcccaac
    36961 atcttcagcc agtccgtcgc caacctcatg cccgggagcg atatcgtcgt ggagatcacc
    37021 tacgacgacc tgctgcccta cgagcagggc cgctacgagt tcgcgttccc catggtcgtc
    37081 ggcccgcgat acatccccgg caagccgacc ggaaaacagg ccgggggctg ggcggaagac
    37141 acggaccagg tgcccgacgc ctcgcgcatc acgccgcccg tgctgaagcc gggcgagcgc
    37201 aacggccacg acatcatggt caccgtcaag ctcgacgccg gcgtgccggt ccaggccctt
    37261 gaatgcccga gccacgagat cgtgaccgag ccggggagcg cgccgttcga cggcccggcg
    37321 accgtgcgcc tggctccgcg cgacacggtg ccgaacaagg atttcgtgct gcgctacacc
    37381 gtcgccggcg acgccccgca gacgggtttc ctggcgcacc gggacgaaaa cggaggggcg
    37441 ttcatgctga tgttccagcc gccggtccag ccgcaactcg accgcatcgc gcccaaggag
    37501 atggtcttcg tgctcgactg ctccggctcg atgtccggcg agccgatctc gctggtcaag
    37561 aaggccgtcc ggcacgcgct ccagaacctc aatcccgacg acacgttcca gatcatccgg
    37621 ttctccgatg cctcgtcgtc gttcgcgtcg gagccggtca aggccacgcc ggccaacgtc
    37681 cgccgcggcc tgtcgtacgt gaacggactc gacggcgagg gcggcacgga gatgctcgag
    37741 ggcatcaagg cggccctcga cttcccgaac cgcgagcgcc gcctgcgcat cgtgtgcttc
    37801 atgaccgacg ggtacatcgg caacgagaag gaaatcctcg ccgccatccg gacgcggctc
    37861 ggcgacgaca cccgcctgtt ctccttcggc gtcggctcgt cggtcaaccg ctacctgctc
    37921 gagcgcatgg ccgaagtcgg ccgcggcgag gtgaagtacg tgctgctcaa cgagaagccc
    37981 gaggacacgg taaagaaatt ctacgagcgc atccgcagcc cggtgctcac gcacatccgc
    38041 gtggactggg ccggccttga ggtcgaggac ctctatcccg cgcgcatccc cgacctgttc
    38101 gcggggcagc cggtcttcgt gctcggccgg tacggccggc cgggcgccgc gacgatccgc
    38161 gtccacggca agatcagcgg acagccggtg acctacgaac tgcccgtgga gctgccggac
    38221 gccgcggacg ggaacgcggc cgtcgcctcg ctgtgggcgc gctcgcgcat ccgcgaactc
    38281 tcgaccgagc tctacggagg cgaggacaag gacgtggtcg aggagatcac cgcgctggcg
    38341 ctgaagcacc ggctgatgtc cgagtacacg tccttcgtcg ccgtcgagga gaagatcgtc
    38401 aacgaggacg gccacccggt gacgatccag gtgcccgtcg agatgcccga gggcgtgtcg
    38461 tacgaaggag tcttcggcgg cgaaccggcg ccgatgcagg tcggagggcg tggcggcggg
    38521 ctcttcttcg gcgcggccat ggcgcgcaag atggtcgccc cggcggccgt ggcccctcgg
    38581 tcgctcgaag ccgacgagga ctcggcgggc gtacccgaac ccaccgaacc gatgaagccc
    38641 ttctccatcg aactgcgcac accggcgcag gggggcgagg aagtgctgcg catcaccgag
    38701 gggggcgaga tctggctgga gcgggggtca acagagcgga tgctcctcgg ccgcctgacc
    38761 cggcggcagg cccgcgagct ctgggcgctg ctcgccaagg cgctgaaggc cccgacggaa
    38821 aaaacaccgt ggacccgcgc cgccgagtta catatccgac tgcggggcga cgacgggcaa
    38881 ccgcaacccg aaacacgcgt ggaaacgagc caggccacgg actttcctcc ctcgccgtgg
    38941 aaagacctgc tcgattacat cgaggtgctc cgggcgggat tctgatcccc gctcgggctc
    39001 gcgggaactc cgcgacgccg ttcagggcaa aatccaccgg cggcgcagcc ggtagaaacg
    39061 cggctggccg tcggcgggcg gcgccggggt cgagggatac aaagggttct cgccgctggc
    39121 aaaagtggcg gccgtcgcgg caacgcggct ggtcaccacc gccatcgccg tccagttcgt
    39181 cagggtgcca gaccattcca acagatccgc cacctggatg cccggcgggc cttccactcg
    39241 cagcaccgga ttcgtaggga ccgccgcggc ccaggccagc acggcttcgc gatccacgcc
    39301 cagcctccgg atttcgcagg agccgtagac ggcccggttg gtaacctgca aggaggccgg
    39361 cacggacagg aacccgcccg ccgccatcaa ctcgcgcagg ttggtgacac aggtccaggt
    39421 cgatccgccg gccacgtact gcccggtcac cacgacgcgc acgaggtagg ccgcgttcgt
    39481 gtccgccccg ccgacccagt cgaaccggag ccggttggtc gtcgccagcg aagcgttcgt
    39541 ctggatagag accaggttcg cgaagaagat cgtctcgctg ccttggggcc gcgcgtccat
    39601 gtagaggttc gtgccgcccg gccgcgacat cagcgccagg cggttggtca gcggaaatac
    39661 cggcgcgttg gaccagcccg cgtcgcagcg gtcgtcgccc tccgcggccc cggcctggtg
    39721 ccgcgccacg aacgtcccgt ggcccacggt gctggtcggc gcgaacggcc cgctgatgac
    39781 ccggacggcg tcgaacggcg cgcgatccac gggaacgacc aggtgggcca cggtggcgat
    39841 cgaggcctgg acgaagcggg cgaaatacgt ccagttcaac gtagaggccg tgtcggaggt
    39901 ggtgtggtag tacgggttga aatcgtcgtc gtcctcaatc gcgaggatgg cgggatagcc
    39961 ctcggtccag aacgagtagt gatcgctgta ctccacgccg ttcgcgtgga tctgcgcggc
    40021 gagtccgctg atgccgtaga cggccaccac gttcgtgaac atcgcggcga tggcgcggtc
    40081 cgcggcatag ccgggatcgg aggtgaggcg cgtgtcgagc cagaggacgc cgtcgctgcc
    40141 gctgtcccag gcgatcatgt ccaggttcag caccccgacg atgttgtcgc ccgccgccgc
    40201 gcactgggcc gcgtacgcct cgctgccgta caggccctgc tcctcgccgg tgaacaggac
    40261 gaaccggatc gtccgctgga acgtgaaatc gcggaacacc tcggccgcca ggagcacggc
    40321 cagcgagccg ctggcgttgt cgtccgcccc gggcgcggtc gcgccggagg gcatgttgtc
    40381 gatgtgcgca cagatcacga cgacctcgga ggaagccgtc gtcccgggca gcgttgcgat
    40441 cacgttccgg ttgctggttc cgccgaccgc ccaggcccgc tgggtcaccg acaggcccag
    40501 cgcggagaaa tactcggcca tgtacgcgtt ggcccgccag ctcggccggc cggaggtcgt
    40561 gtagcgggtg cggatgttcg tgtacgagcc gtccgccacg caggccgcgg ggccggtcag
    40621 ctcgttcatc gcatacgcca ggccgttcgt cgtgacgcgg gccagcatgt cgcgcacgtg
    40681 cgcgctggcg gtgagggccg tcggcgcgtc cggcagccgc cgggctgtgc gcggggcaac
    40741 gaacgccagc ggccggtcgc cgagccaacg cacttcgcag cccccttccg ccagcgccag
    40801 ggcatccgca tcggtcgcgc cgcggatcag cagccggcgc ccgtcgtcga gcaggatgcg
    40861 aaaccgcgcg gccgccgcct ccctcgcgcc gggtcggcgc ggataggcca gcaggtagcg
    40921 ggcgccgtcc gcggccggat cgagaacctc gtggggccgg ccggcggcct ccagttcaga
    40981 ctccgtggcg cgggtcagca cgtactcccc gcccgcggcg tcctgcaggt gggcgcacac
    41041 cggcagcggg aggtccgcgg ggcggccggc gatcgggatt cgcgccagta ccaagcccgg
    41101 ttcctgcccc gcacacgcca agggcagaag gaatagcccc acccctccaa gccatcgctg
    41161 aaaaagcccg cgcatgcctc ctatataccc gaaagggcgc cggtcgataa gccttatcac
    41221 cgcgctgggt ccgggacggg gaagaccgcc tcccgtgtcc ccctggcgga gcgcgggcgc
    41281 cgcggctcca tctttttcca aaaatagatg tagacagacc ggtttttata ttatatagat
    41341 acaatatagg agttaaataa tatgaaaaag atcaacgacc tggaggccgc gctgatgggg
    41401 ctgctgaacg agaaaccgat gcacccctac gagatcgaga aggtgatcgt cgaacgggac
    41461 atgcgctggt ggaccaccat ctcgatgtcg tccgtctata agctgttgcg caagctggag
    41521 aaggagggcc gggtgaaaag cgaggtccgg ctgaaccggc gcaacctggc gcagaaggtc
    41581 taccacctca cacccgcggg ccggaaagcc ctggtcgccc gcgtgaaggc gctggcctcg
    41641 gagtggaccc ggcccgtctg gcccgtggat atcgccttaa gcaatctccg cctgctgacc
    41701 gacgaggagg tcgccgcgtg tttccgccag tacgaggact cgctggacaa gatgatcgaa
    41761 tgctacggcc gcctgcagga atacctggag gcccactgcc cccttccgaa cgtccaactg
    41821 gccgtccggc cgcttgcttt gatgagggcg gagaagcaat gggtccgcgg gttcatgaag
    41881 caatacggca agaagcgcaa gggcgggcaa gcgggctcgg ccccgtcccg gtaacgtcac
    41941 gaacgaaaac cggaggaacc cccatgccag acttcatccg cgtcgtcggc gctcgccagc
    42001 acaacctgaa gaacgtcagc ctcgatattc cgaagcaccg gatcgtcgtg ttcaccgggg
    42061 tttcgggctc gggcaagtcg tcgctggtct tcgacaccgt atgcgccgag gcgcagcggc
    42121 agctgatcga gaccttcagc gcgttcgcac gccggcgcct gcccaagatc agcaagccgc
    42181 acgtcgagga gatcgagaac ctctccacgg cgatggtgat cgaccagaaa cgcctcggca
    42241 cgacgctgcg ctcgaccgtc gggaccgtga cggagatctt cacgcacctg cggatgctct
    42301 tctcccggtg cggcgagccg ttcatcgggt tctccaacgc gttctcgttc aacaacccgg
    42361 agggcatgtg cccggcctgc cagggcgtgg gcaaggagat ggtggtggat atggaccggc
    42421 ttctcgaccg ggagaaatcg gtggaacagg gcgcagtgct ccattccaac ttcaagcccg
    42481 gcggctggta ctggaaaggc ctgatgaagt gcgggttttt cgacccgcgc aagccggtga
    42541 aggacttcac ggaagaggag ctgcacaacc tcttgcaccg cgcgaagacg cccttcacga
    42601 atatcctccg cggcgaggag tacacgtccc agtatgaagg cattgtgacc aacctcaagc
    42661 gacggttcgc gaaccgggac gagggcgagg acgcctacac gcgcttcttc acctaccagg
    42721 actgcgccga atgccggggt tcgcggatca gcgaggcggc ccgcggcgtc aaggtccagg
    42781 gcgtcacgat cccggagctg gtcttccggg agctgcccga cgtgctgcag ttcgtcgagt
    42841 cggtccgcgg ccccgtggcc gaaccgctgg tccgcaagat gaagcagagc ctggccaacc
    42901 tgatcgacat cggcgtgggc tacctgtccc tgcaccgccc ggtggccacg ctgtccgggg
    42961 gcgagtcgca gcgggtgaag atggccaagc agctctcgtg caacctcgtc gacctgatgt
    43021 acatcctgga cgagccgagc atcggcctgc acccgcgcga cgtggggagc ctgctgcgga
    43081 tgctggtcga gctgaaggaa aagggcaact cgctgctggt cgtggagcac gacccggcgg
    43141 tgatccgggc ggcggagtac gtcgtggaca tggggcccgg cgccgggtcg cagggcggcg
    43201 agatcctctt tgccgggccc gtggcggaat tgacgacgtc gggcacgctg acggcccgct
    43261 acctggagcg ccgccgggac ggcgcgcacg cccggcgcgc gccggcgggc tggttccaaa
    43321 tcagggacgc cgccgtccac aacctgaagc gcgtctccgt gcggatcccg gccggcgtcc
    43381 tgacctgcgt cacgggcgtc gcgggatcgg gcaagagcag cctggtgcac gacatgttct
    43441 gccgcgagca tcccgaggcc gtcgtcgtcg accagtcgcc ggtcggccgg tccagccgct
    43501 cgaacccggc cacctacgtc ggcgtgttcg acgccgtgcg gcgggaattc gcggcggcga
    43561 cgggcgcctc ccccgccctc ttcagcttca actccgacgg cgcctgcccg aagtgcaagg
    43621 ggctcgggat ggtcaaggtc gacttgagct tcctcgacga cgtcaccatg acctgcgacg
    43681 agtgcgacgg gaagcgctac aaggacgagg tcctcgccct ccggcacaag gacaagagca
    43741 tcttcgacgt gctggagatg accgtccgcg acgccctggg tttcttcgag cacaaggaaa
    43801 tcctgcgcaa gctgcgcatc ctcgagcgcg tgggactgga ctacctggag ctcggccagt
    43861 ccttgagcag cctttccggc ggcgaggcgc agcggatcaa gctggcgagc gagctgcaca
    43921 agaagggcaa catctacgtc atggacgagc cgaccaccgg cctgcacatg gcggacacgg
    43981 accggctgct cggcatcatc ggccagctcg tggctcgcgg caacagcgtg atcgtgatcg
    44041 agcacaacct cgacgtcatc cggcaggcgg actgggtgat cgacctcggg cccgacggcg
    44101 gggccgccgg aggctacgtc gtggccgagg gcccgccgga ggcgatcgcg gcgaatcccg
    44161 cctcgcacac ggggcggtat ttgaaggaaa cgctctgaag acagacgccg gcgagcagcg
    44221 gggctacgct ttccgcgcga tgcgccgtcc caccaggacg gacgccagcg tcgtcgcggc
    44281 gcagatgaac gagccccatg cgagatccac gatcgtgacg atccacggcc agccggcgat
    44341 cacggcctgg ttcgtcaggt cgaacgtggc gtaggtcacg aagccgaaga acccgccccg
    44401 cgccgcggcg cggccgagcg aagcggtccg caggcccggc tccacggcaa agaaggtcag
    44461 cccggcgatg aagaccaggt agaagagcag cgccgcgccc cagaccggcg gatcggccag
    44521 catgaacccg atgtgcgcct tgtagaaacg cggcgagatc acgcccagcc agacgctgtc
    44581 gagcaggagg aagaggacaa aggtgatcgc aaagcgtttg acgaaggtca tggccccgct
    44641 cctgcggatt tcagggtatc ttcaccccgg tccggtagaa aatcaaatcg cgggtgttgg
    44701 tgacggtcag ggtgacggcg gcggcgttgg aggcgcccgg ccggtcgagg tgggtcgcgt
    44761 tccaggcgga ctcgcgcagg ttggtggacc agaacacgtc gtacacgcgg ctgttggtcg
    44821 tcggcgggcc gacggacaac gacacgacct ggccgtcgcg cgaatagttg gtgatcacgt
    44881 tgggataaaa cgagcccgcg ttggtgggcg acgtatccgc ggcgtactct tcgtcgttga
    44941 gccgtccatc cccatcgttg tccgcccccg cgacggcgcc ggtcgtgttg ttgaagaacc
    45001 gggattccca ccagtccggc aggccgtccc cgtcggtgtc cgattccagg tcgtaggccg
    45061 gccgtatgat cgtgaccgcg tccaccgcct gcgctcccgc cggcggggca tgcgacgacg
    45121 tgatcttgag ttcggtaatg tagacgttgt aaggggtccc tccgcccgcg ctggcgttcc
    45181 acacgcggac ctgggtcagg ccggccgccg ccgccgagga caaggtgccg gtgcgcgtga
    45241 aaccgcccgc gcggaacagg tacgaggtcg gcccggtcag ctcgaattcc gaaagcagcc
    45301 cgctgtcggt ccagctgacc gtcgtggtct gcgccgccgc cgcatcggtg atccggtact
    45361 tcgactcgcc ccctacgaaa tagaactcga acagggtgtt cccgtagtca tcgtggagcg
    45421 tccaccccgt gctccggccg gtatcgatga agttgttttc aaagccgatg cgcaccacgt
    45481 ccccggcctg gagcggcgcg gcaaaagggc ggcgggcttc cgagagtccg ccgttattcg
    45541 cccacaggcc ccaggcggac gaggccaggt tcaggttgga actgccgggc agcgtggcgt
    45601 agtagtggcc cgcgctggcc ccgccgacga gcgtccaccc gccgccccac cccgtcccgc
    45661 cgttcgagcg gttggtccag ccggcggcgt atacggaatt cgacgcggtg tccgacgcgg
    45721 aggcgttgcc gggccggtta gtcgccgcca cccggatata gttggtgccc acctcgaccg
    45781 gcacggacgc gagggcccag cgcgcggtcg cggcgccgct gccggtttgc ccggtgagct
    45841 ggttgctcca ggccacgaac ccggcggtgg ccacggtcgc cgtgccggac agcgtgtact
    45901 ggctcgtgcc gtgcggaacc gagatcgtgc cgacgggatt ggtcagggca atgctgggcg
    45961 gcgcgggcca gcagttggtc acggcgacgg accagttctg gccgtagtgg ttgtcccacg
    46021 tccccgcccc gtcatggaaa actaaattca ggcggcgcgc gccggccggg atggaatagg
    46081 aagcggtcca ggttcccgcc gggctctcgg tcatggccgt cccggggctc gacacgtcca
    46141 gccagccgtc gtgcccgatg aacaagtaga cgctcgcggc attggtcagc gggccctcgt
    46201 tctcccggta cgtgacggtc accggcacgc agccctgcgg cgcggacggg ctcacggtga
    46261 cctcggcggg cgtcccgccg ccgcacccac tgaccgagag atgccagtcc gagcccaggt
    46321 tgttgtccca cgtgcccgcc ccgtcgttaa agaccaggtc cagccggtag gtcccggggg
    46381 cgatcgtgtg cgtgtactcc caggtcccgc cgacatggtt cgacatcggc gcgtccacga
    46441 cgtcctgcca ctcgttgcgg ccgaggtaga ccttcacctg cgcggcgtcc ttgagcggat
    46501 gatcgagggg atcgtacgtg atcttgacct ccacgcatcc gccgggcgcg gccgggtcat
    46561 acgcgacggt ccccggcgtg acgtgctcct ctttcgagaa tatctgcacg ctgtacatcc
    46621 ccatgttcac ggcagcggtc ggcggcgagc cggcggcgac caccgaggcc ggcccgatgt
    46681 tcccgaagtc ggcctggtag ttcgtcgagt cgccgttgaa gtgcgagtac cacgtgcccg
    46741 cggagggaaa ctggatgtag tagttgttac tgttgcggac ggtcgccgag aagttcgcca
    46801 ccacgaccac gtcgtccgcc ccgccgcccg cgtcccaccg cgtgtaggcg atgaccttgt
    46861 tcgcgttgtc gatgtgatgc acgttgatcc ccgtgccctt cagcccgccg gtggcgccgg
    46921 ccacgttccg gcgcagaagg atcatgtccc ggtaggcccg gacgatcccc gagtagctat
    46981 tcgtcaggct ccagcgaagc gattgctccg cgctgaacgt ccaggtctcg tgcatctcct
    47041 ggccctggaa gatcatcggc acgcccggcg ccgtcatgac gatccccgcc gccagcaggg
    47101 cccggcggcg cgcccagatg ctgtccgggt tgcccgcgtc gatatcgcgc ggcagccgga
    47161 cgttgctgtt gaggtcgccc accgagtcgt gcgactcgga gaagatcacc cggttgtggc
    47221 tgggccagcc gttcagcagc gaggccaccg tgctcatgtt ccggtcggcg tcgctggagc
    47281 gcgtcacctg gtactcgaga tcgctgttga acccgacgtg ccactggctg tcgaagttca
    47341 cgctgtagtc gaacgcgttg tcctcggcga tgcggatctt gccggggtac gactgcccga
    47401 tttcccagtt gatgtcccgc agcatcgcct cgccgtccgc gttgtggttg gcgccgttgt
    47461 tgtagtagat gatgttgaag acggagtccc accggaagcc gtccacccgg cactcctcca
    47521 gccacatgaa gatctggtcg cggacgtagt cgcggacctc gctgcgcccg tagtccggcc
    47581 gggtgtcgcc ccaccacgtg ttcgccttgg cgtattcgtt gtagaaataa atgccgccca
    47641 ggttgttctg gctccacccg tcgaaccgcc acaggtcgag gtcggacggc ccatagtggt
    47701 tgtgcacaac gtccaccagc accgcgatgc cgcgctcatg gcaggccttc acgaagcgct
    47761 tgaaggcgtc ggggccgccc agcgcgctct cgatcgcgta cgggtcagag ctgttatagc
    47821 cccagctctt gtcggccggg aactcgaacg ggggcatgac ctccaccgcg ttgatgccga
    47881 ggttctgcag atggtccagc cgctcgatgt ccttgtcgaa cgaactcggc acccaggact
    47941 ccgcgttgta ggtgccggga tgcatttcgt agatcaccag ctcgttccgg tacggcgtgc
    48001 tgaaggagtc ggagccccag tcgaacgcgt ccgggtcgta gaggacggaa ttgcccgcgg
    48061 agttgaccac ctggcgcgcg cgcggatcgc gcttgtcgaa gctgttgttg accctgacct
    48121 tgtatttctg ccccgcttgg gccccgttga tgtccttcga ccacaggccg ccgccctcgc
    48181 tggtcagcgc cgtcgtgccc caccccgtga actcgccctt gatcccgacc gcggttgcgt
    48241 tcggcgccca cacgcggaac gtgtatcccg tgccggcggc atccgcgtag ggcgtgggcc
    48301 ccatgccggg acgcgacgac tgggcgcgaa catggggagc aagagtaatg atgcagaata
    48361 gaacagcccg aattacgacc ctggaaactc gtaagggcat ggttttcggt aaaacatttt
    48421 accgcttctc ggacttgccg ttaagagcaa cccgcaaaaa tgttttacca ccgctcgtaa
    48481 tgcaggattt cggctgtttc cttgcgcgat ttcggacgag gcggcgcatc cggaaagcca
    48541 agaggagtca tggccagcgg ttcgatccct tcgggcaggc ccaggatgtc ccgcgccttt
    48601 ttcggatcga atgcgccgat ccagcaggtg cccaggccaa ggtccgccgc gcacagggtc
    48661 atgtggtcca tcgcgatcgc gccatccacc gagacgtagt tcttgccatc ctgccggacc
    48721 caggctttcc cgggttccac gcacacgacg aggatcgccg gcgcctttcc gaaccagtcg
    48781 cgcgggtagg cctgctgtag tttcagccgg gccggctctt cgcgcactac gatgaaatgc
    48841 cacggttgct tgttcgccgc ggagggcgcc agccggcccg cctccagcac cttcgccagc
    48901 caatcctccg gcacggggtc gggcttgtat ccgcgaatgc tgcttctctt cgccgccagt
    48961 tccagaaagg tcatggctcg ttctccgctt ggatcatgag ggaagatacc acggaatccg
    49021 gaagaaacta cgaaaggcgc gaaatacgcg aaaacgaagg gccttctttc gcgcctttcg
    49081 cgtgtttgta gttccctact ccctgacttg tccgttgccg aggatgacgt acttgtacgt
    49141 ggtcaactct tcgaggccca tcggcccgcg ggcgtgcagc ttgtcggtgc tgatgccgat
    49201 ctcggcgccc atgccgaact cgccgccgtc cgtgaaccgc gtcgacgcgt tcacgtacac
    49261 ggtcgcggag tccacttcct gcacgaagag cttctgcgcg gcctcggact gcgcgacgat
    49321 cgcgtcggaa tggcgcgagc cgtaccggtt gatgtggtcc gccgccgcct tcacgttcgc
    49381 cacgacgcgc acggcgagga tcaggtcgag atactcctcg gtccagtccg attccacggc
    49441 ctccttcatc gagggcgcga tcgcccgcga ggcggcatcg ccccgcagtt ccaccttgcg
    49501 cgcggccagc cgttccgcca tccggggcag gaacgtggcc gcgatcttct cgtggaccag
    49561 caccttctcg atcgcgttgc agacgccggg ccgctggcat ttggcgttct cgatgatgtt
    49621 cagcgccatc tcggcgtccg cgctctcgtc cacgtagacg tggcagacgc ccttgtaatg
    49681 cttgatgacg ggcacgcggg cctgctcggc cacggcgcgg atcaggccct cgccgccccg
    49741 gggaatggcc aggtccaccc gcccctccat ctggaccagc tcgcgcaccg cgtctcggtc
    49801 cgcggtctgg accagctgga tcgagcccgc gggcagcccc ttcttcgcgc cgccctcctg
    49861 catggccgcg gcgatggccg cgttggaatg cacggcttcc ttgccgcccc gcaggatcac
    49921 ggcgttgccc gtcttgatgc acaggctcgc cgcgtcggcc gtgacgttgg gccgcgattc
    49981 gtagatgatg gcaatgacgc cgatgggaac gcggatcttc tgaatgacca gcccgttggg
    50041 gcggatccac cggctgatgc gcgtaccgac gggatccttc aggaccacga cgttccgcag
    50101 ccccttgacc atgccctcga tccgggcgtc gttgagcagc agacggtcca gcaacgcgcc
    50161 cgacaggccg gccacgcggc cggcttccat gtcccgggta ttggcctcct ggatgcccgc
    50221 gcggcgcgcc tcgatctcgg ccgccatggc ctccaggatg gaattcttgc ggcgggagga
    50281 caactgcgcc agggcgcggc aggccgctac cgccttgtcg cccatcgcca gcatttcctc
    50341 gtgaaggctc atatggtccc cctcgtcgcg ccctggagca cgaccatgtt gtcacggtga
    50401 acgacttcct cgaagtccgc cgcgcccagg atggccgcga tctccgacgt cttgcggccc
    50461 tggatccgcc ggacctcccc gctggaatag tccgtcacgc cgcgcgcaaa cgccggcccg
    50521 ccggccgcgc ggatgttcac cacgtccccg gcttcgaact cgccctcgac ggctttcacc
    50581 ccgatcggca ggaggctccg tcctttttcc accacggcca gccgcgcccc atcgtccacg
    50641 accaggctgc ccttggcctt gtggaagaag gcgatccatc gcttgcggcc gctcaagccg
    50701 gacggcttgc ccgcggccaa aagcgttccg atatcgtcgc ccgcaagcac cttcccgatg
    50761 gcgcccgcct gacggccgtt ggcgattacg accggtgtcc ccacgcccgc gacggtatcc
    50821 gccgcctgga gcttggacat catcccgccc gtggacagtt cgctcccctt gcccacggcg
    50881 agggccaggg ccttctcgct gacgcccttg aggaaaggca cgcgccgggt gcgcccgccg
    50941 gccgccggcg cgcggaagcc gtccacggtc gtcaacagga tcagcaggtc ggcctggatc
    51001 agcagcgcga cgagcgaggc cagcaggtcg ttgtcgccga acttgatctc gtccacggac
    51061 accacgtcgt tctcgttgac gatggggatg atgccgtggc gcagcaggtt catcatcgtg
    51121 ttgcgggcgt tcagatgccg ctgccggtcg ttcaggtccg cgtgggtgag caggacctgg
    51181 ccgacccggc acttttcctc ggcgaagagc cggtcataga tggccatcag gcggctctgt
    51241 ccgacggcgg cggccatctg gagatcgggc aggtgcgtgg gccgcgtttt caggccgagc
    51301 acggacatcc cggcgccgat ggcgcccgag gataccacga cgatctcccg ccctgcccgc
    51361 cgggcgacgg ccaggtcgcc caccagcgcg cgcaggcgac gcaggtcagg ccggccgtcg
    51421 cgttggacca gcacccggct gccgattttg acgaccacgc gccgcgtgcc accgaatgtc
    51481 gcgcgcattt ggaggttttc gtccattttt cctcgaaaaa cggactattc ctagcagacc
    51541 ccgccccgcg tgtcgagcca ggacgggcca gcccggataa tgcgcgattg gcgcgcatta
    51601 tccgcccaaa gggccgtgat gtgcccgcgt tgcgggcaca aacggggctt gccacgttcg
    51661 tattgtcgaa tccttgattc gacaatacgt cacgtcttct ggcacgcgaa ttgcctatgc
    51721 aattcgcgtg cccagaagcg cttattgcgt gagcgtctct acgctcatgc aataagcggg
    51781 ctagtcggtc agcgtggcgc gaatacccaa ttcctgaagg aggcccacgg gggcttgctt
    51841 ccctcgggtc acccatggcc gaaggatctc gcgggcaatg cccgcgacat gcgtgccgtg
    51901 gccgtttatg tcctccggga gcgcgtcatc gtcgatgtag ttgtatccat tggtaacgcg
    51961 ggtgtgtcga aatccgaatt ggtggagcac aggcccgtgt ccacgaccgc gatcgtgatc
    52021 gcggcggtgc cggtggcgaa atcccaggcc tgggtggcgc cgatgatccc gtggtgccac
    52081 tggctggagt agagggaatc gttgggcacc aggcctccgc tggccacgcc attggcctgg
    52141 gcgcactcca ccacgccgag gcgccgcaac tggctgaagg ccaggtcgat atcctgtgcc
    52201 gacctcggcg cggagccctt ggtccggccc acctccagga atcggtccag gcccaggcgg
    52261 ctgaacgccg cgggatcgcc ggctcaaacg atggccaatc ccacaaaccg aatggtgcac
    52321 acgtatttca tggaacttcc tccctcgttg attccaatgc ccccagcgag atcccgccgg
    52381 gctatccgga aactgaaacg gtaaatacgc cggcccgaca aaagcgtaca ggcttccgcg
    52441 tttcgacgga cagggatccg gcggatcggg atcagcgggc ggccaacgcc agcagtgcct
    52501 tgcggagatc gtcggcaggc caagccagcc ggtaccaacc gctcttcgtg tccaggaaag
    52561 cccgtgcgcg taatttctgc aggtgccggg tgagggcggc ggaggatacg cgcatgcggg
    52621 ctttcagatc gccgtatctc atcggtcgtc gcagccattt cagaatctgc tgccgtcttg
    52681 gatgcgtggc cgccgtcagc gtcttcagga gcgcgtcgat ggaagcgccg gattttccca
    52741 gagcactcac gacggcttgc agcagcaggg cggattccgg gagcgagcgg tcgcctcccg
    52801 cgcgatagaa gacatggcgg ccgtttcgcc ggacgttcaa caagccccgg gcattcaaca
    52861 tgcgaagcga ctggctggct gtgccgagtg aacagtccat gagccgggcc acgcccgaga
    52921 catcgagttc cccgcgagcc accagcaatt gcagcatggc caatcgccgc ggattcgcca
    52981 agacccggca agtccgccac aacgtaggcc gaagcggaac ggaggtcgat tccttcatgc
    53041 tcacacttct agaagtgtga gcatgacttg tcaacattta tccacttatc aacaaagagg
    53101 ttattgtgga tcatcacgat ggtcgcgtgt tgcatcgtgc tcatacttct agaagtgtga
    53161 gcacgagccg ggtgcaggtt tcaatccccc gtccccgcca tggaagccct tcaggccccg
    53221 ggaccggcgg cgtcattcac gggcccggac ttttctcctt ggcaagggca accgcgcgac
    53281 cgcctcctgt aacaggcccg agatggcttc gccgccggac aaccggagca gtaacatggc
    53341 cgggtgaatc ggttctgccg cccgcacttc cttgaacgac ggcagcagca atagcgccgc
    53401 cagcacccgc agaaaggcgg acacgacgaa cagggcgggc aacggcgaga gcaggttgat
    53461 gttccagccg ccgaatcgat agtgcgtcgg caatcgggag gccagccatc cacccagcaa
    53521 cgtgccgcac aacaaggtgc aggcgccgtt cagaatcgtc atgtaggccg tcaccctcgc
    53581 gcgcttcggt ggcgtgacgg cgtcgaaggt gaaattctgc gtcgcgatgg cgaatcccga
    53641 ccaggccaca ccggcaaaca tctggatcag gagcaggagc cagtaattcg tggtcagggc
    53701 aaacagcagc gggatcagcg gcagcaggca gctcgtagcc acgatcaccg accggttccc
    53761 gtgccggtcg cccatccgcc cccaccagcg caccagcagg aactgcacgg cctggaatgc
    53821 ggcggcgttg atcgtgaact ccgcgtacga ccacttcaga tctcgcagaa aaaacaccgt
    53881 gatgaacggg gaagcgacgc tgatcgcccc gttcatcagc gcggcgccaa cggcaaagcg
    53941 cgtgaaatta gaatgccgga tgcgccggag gaagtcccag aacgagaagt aatcctgcgg
    54001 cgtcggccgg tacggtggat cgtaatgacg ggtcagcagt tgcacggaaa cgagtcgggc
    54061 catgaaggcc actccgaaca ggacgccgaa tcccgcccag accatcccga tccgctggta
    54121 cagagagaga atcccgcccg ccgccagcgt ggacataaag accatgagga tcaccacccg
    54181 gccgcgacga ccgaagtaat ccccgcgctc agcgggcgga acggtctcgc tcatcaggct
    54241 cgtccacgcc ggcgccacgg cgttgcccgc cccgatcgcc agcgcggcaa acacgatcac
    54301 cgccagcacg tcgaactgcg ggaagagccg cgggatgaaa aacagcggta cgtagaccag
    54361 cgcctggatc aggcagaaga gtacgatcca gtcccgccgc cgcccgacac gatcggtcaa
    54421 cgccgcgcca gccatctgcg agatcgcccc gatcaacgcg ggcatggtgc ccaggaaggc
    54481 gaccgcgcgg ttccccgcct tcaggaaaac cgcgaagggc atgatgtagg tctcgccaaa
    54541 gcccgacatg agggaccaga tcgatccctc gaccatcgag accttcatcg ttcgccgccg
    54601 ggccgcctcg atttcctcgg acgacccggg cgtcggcgcc ttgtcgaata gccatccaag
    54661 cgatttcatg gtctctcaag gtgacacggg cggcccgccc gtgtcatccg gcggcgacga
    54721 agagccgccc tccaaggctt catggatcaa tcgggccagc gctgtgccga tgcctggcac
    54781 ggccgcaatt tcctcgatcg gcgccttccg catgcgaagc acggagccga agtgcttgag
    54841 cagggcctct ttgcggttgt cccccacccc ctctatctcg tcgagaatcg actcgcggat
    54901 gcgccgcagg cgcaggcggc gatgataggt caacgcaaac ctatgcgctt catcccggat
    54961 ttgctggagc acccggagcg cgggagagtc ggacgcgaaa acaagcggcg cggtgttcga
    55021 ccggacatcc gcgtacagct cttcaaagcg cttcgccaag ccggcggccg gcagatcggc
    55081 caggcccagt tcgtcgagca cctgccgggc cgcgcgcagt tgggtgaggc cgccgtccac
    55141 gagcacgagg tcgggccgcg gcattttttc cgccaggacg cggccgtagc gccggcggat
    55201 gacctcggcc atcatcgcgg ggtcgtccga gccttcgacg gtgcggatgc ggaacatccg
    55261 gtagcggttc ggcgcaggct ggccgtccac ggcgcagacc atgctgccga ccgcgtgcgt
    55321 gccggagatg ttggagatgt cgaagcattc gataacgctc ggcggcgcgg cgagagccag
    55381 gacgcgccgc agttcctcca gtccggcccc ggcctcggtc gccttccgga caggggattt
    55441 ccgtccccgc gcccgctcgc gaatggcccg gcgcagcagc aggcaggtat cgcgcagggc
    55501 cgccgccttc tcgaaccgca gctcggccgc cgccttttcc atgtcctgct gcatctcctt
    55561 caggaactcc ggccgctcgc cgcgcaggaa cgccagcgcg gtctcgaccc gctcgcggta
    55621 ctgctcgggg ctgaccttca ggatgcacgg cgcggagcag tagcggacga tatcgttgtg
    55681 gcagtgcttg tggtcctcgg gccccggcac gcgcgggcgg cacacgcgca gcccgaaccg
    55741 gcgctcgacg aactccaggg ccgcgtagac ggccggcgag gaggcatacg gcccgaagta
    55801 ggtcgcgcca tcctccttct ggatccggca cgactcaaag cgcgggaacg gctcgttcag
    55861 gttgacccgc agcaggagaa acctcttatc gtccttcatc aggacgttgt accgcggccg
    55921 gtagtccttg atcagccggc cctcggtcag gatggcctcg gcctccgtgc gcgtgacgag
    55981 gatgtcgaaa tcctcgacgc tgcggagcag gccgcgcagc ttgggctccg cgctgcgcag
    56041 cgtggcctgg cggaaatagg actgcacccg gtgccggagc gaggcggcct tgccgacgta
    56101 gatgatcttc ccgcgccggt cgcgcatgag gtacacgcca ggcttgtcgg gcagggcctg
    56161 gagcttgtcc tgaatcttcg acgaccacgc catgggccgg ctactcccgc gccagctccg
    56221 cgaacccgag ccgcgcctcc tcgatgaacc gacggaccag cgccccgtcc ttgcgcccgt
    56281 ccgggccctc gacgccggtg tgcgcgtcta ctccggccgg gcgcacgcgg cggaccgcct
    56341 cgcgaacgtt gcccgggcgc atcccgcccg ccaggatcac cgggcgggcc gaaagcttca
    56401 cgagctcgac gctgacggcc cagtcgtgcg tcttgcccgt cgcgccgctc gcgccggtgc
    56461 ggggatcgaa cgtgtcggtg atatacgcgt ccacgaacgg atccagctcg cggacctgcg
    56521 ccacgagggc tcccacgtta tccggccgca cgacgaggct cttgatcacg aacaggcccg
    56581 gatcgcgctc ccggagtcgc gccgcctggt ccggcgccag cccgccgtgc agctggacgt
    56641 gccgcgcccc gagctggcgg cagaactcga cgacctcgtc cgcgcgctcc aggtacgtga
    56701 tcagcacgcc gggacaggtc gggggcagcc tgcggatgat ctccgaggcg gccgcttcgg
    56761 agaggtcttc cttgtgcacc gggaggcgca gcggaaagcc gaggtgcgtg gccccgagcc
    56821 ggaccagcag ctccgcctcg gcccggtcga tcacgccggc gatctggatc gcgggataag
    56881 gcaggcttcg agattgattc atctggaaga gtataccggg ttgcgcgcga aatccaagac
    56941 agtcggaaaa ccacggagga cacagagggc acggagccgg gagaagttcc tccgcgatct
    57001 ctgcgccctc cgcggttcaa gcggttggag tttcagtcca gcacttcgcg aatggcgtcg
    57061 gcctcgtccc gatcgatcac gccgtcgccg tcggcgtcga actcgcgttc gatgtccgtg
    57121 ttgacgacgg cgcggccatc ggtctcgatg acgcgcaagc ggtccttcaa gagctcccgg
    57181 ccctcggacc attccaggta cccgtcgccg ttggcatcgt acttccgctc gatatccgtg
    57241 ttcaccacgc ccttccagcc ggcccagtag gcgcggttct cgtccggggt gatcaccccg
    57301 ttgtggtccg tgtcgagaac cttgtatcgc caggcatgca cctcggcggg ctcaacccag
    57361 ccgtcgttat tgacgtccac cttgcgttcc caggccgaat tgactttggc ccggtgccgg
    57421 gcctcccagc gccgctccac gtgccgttcg gcaggcgtca ccacgccgtc gttgttcttg
    57481 tcggcgtgcc gccggcgcgc gccccaccac ggccgcgcct gggcgacctc caagccgacc
    57541 accgagaaaa gcgccgccac gcataccgcc gtcgccacat tcgctttcat cgctccccct
    57601 cctttttgtg ccagtatcgc acagtccata ctacgcgcgg tgccttattt tattgtgcgc
    57661 ccccgcgcgc tccgcgacaa atcaggcgtg tgtcacgggc cggaacttga tccggcgcgg
    57721 ctggtcggcg gcgtcgccga gggtcttgcg gcggaactcg tggtaggcct cgaagttgcc
    57781 ctcgaaccag cggacctggc tgtcgccctc gaaggcgagg atgtgcgtgg cgatgcggtc
    57841 gaggaaccag cggtcgtggc tgatgaccac cgcgcagccg ccgaagttca gcagcccctc
    57901 ctccagcgac cggagcgtgg tcacgtcgag gtcgttggtc ggctcgtcga gcaggagcag
    57961 gttgccgccg cgccgcagga gcttcgcgag gtgcacgcgg ttgcgctcgc cgccggagag
    58021 cttgcctact ttcttctgct ggtccggccc gaggaagttg aagcgcgcgc agtaggcccg
    58081 cccgttcatc cgcttgccgc ccaggtccac tagttcctcg ccgccggtga tctcctcgta
    58141 gaccgtccgc tccgcgtcca gcgcgtcgcg gaactggtcc acgtacgagg gcaccaccgt
    58201 cgagccgacg gccagcttgc ccgccgtggg tttctcctgc ccgacgatca tcttgaagag
    58261 cgtggtcttg cccgcgccgt tggcgccgac gaccccgacg atcccgccgc gcggcaggtc
    58321 gaaggtgacg ttctccatga tcagccggtc gccgaaggac ttggtcagcc cctcggcccg
    58381 caccacgatg tcgcccagcc gcgggccggg cgggatctgg atctcgagcg agtcctcgcg
    58441 gacgtccacc tgttgagagg ccagctcctc gtagcgcttc aggcgcgcct tgctcttcgc
    58501 ctgccggccg gacgggttca ggcgcaccca ctgcagttcc tgctccagca ccttgctgcg
    58561 cgcggcggcc tgtttcttct cggcggcaag gagggcctgc ttctgggcca gccaagcctc
    58621 gtagttgccc ttgtagggga aggtgtggcc gccctcgagt tcgaggatcc attccgtgac
    58681 attgttcagg aagtagcggt cgtgcgtcac gaccaccagc gtgccgggga actgcttgag
    58741 gtgcgcctcg agccacgcta cggactcggc gtcgaggtgg ttcgtcggct cgtcgagcag
    58801 gagcacgtcg ggattggaga tcagcaggcg gcagagcgcg acgcgccgct tctcgccgcc
    58861 ggagagcgtc gtcaccgacg tttccggcgg cgggcagcgc agggcctcca tggccatgtc
    58921 cagcttgtgg tcgatctccc acaattcgtg ggcgtcgatc tggtcctgca ggcggccaag
    58981 ttcgtcgttc agcttctccg cctcggcggc gggcgggctc ttcccgagct tctcgcagat
    59041 ttcgtcgtac cgggccaata cggcgcgcgg ctcggccagg ccctcggcga tgacctgctc
    59101 gacggtcttc gacgggtcga gcggcggctc ctgcggcagg tggccgatct tcgcgttccg
    59161 ggcgatgtgc gcctcgccga ggatatcgcg gtccatgccc gcgaggatgc gtaggagggt
    59221 ggacttgccc gagccgttgc cgccgatgac cccgatcttc gcgccgaaga agaacgcgag
    59281 gttcacgtcg tcgaggacgt tgaccttgtt gtactgcttg gtcagccgga ccaggttgaa
    59341 gacgtattcg cccgccatgg ggccgggatg aaagcacagc cgcgccgcga aatccagacc
    59401 ggtttcgcgg accggagcgg cgccgcgctc cggcttgaca tcgttatgtc ttattatacc
    59461 atacgaacct cgtggactct ccacagatca caggcagggg aaacgccgcg gcgttcacgc
    59521 tgatcgaatt actcgtggtc atggccatcg tcatcgtgct ggccgtcctg gccgtgccct
    59581 gggccttgcg ctcgatcaac gcggcgcgcg agcgggcatg cgcggccaac ctccgcgcct
    59641 ggggccaggc cttcatggtc ttcgccgcgg agcaccaggg cctgctgccc caccccgacg
    59701 accgccggcg ggacggcggg gccccgggat cgtcctccca tcccgagcac gaccagggct
    59761 acatcgacct cctaccgccc tacatgggcg agcggccgtg gcgcgacatc ccggaggggg
    59821 aaaaaccccg gggcgggatc tggcagtgcc ccgccgcgaa gccccggccc gattccgagt
    59881 acgactacaa gccgtcccgg cagggctacc acagctatgc catgaactcg tacctggcgc
    59941 acgatttcta cttcgggctg ccctgggacg ccgagctcca gcccagcttc ctttacctgc
    60001 cccgcgcgca gaacacggcg gccacgatcc tgatgttcga gcagacactc gatccgggcc
    60061 agggctacgg ccaggccggc tccttccgct ccgccggcta ccagacggcg gaggacgcgc
    60121 gggccgtcac ggagcggcat cgccggagcg ccgagggcct cggcggcaac gtgctgtacc
    60181 tcgacggcca cgccggctgg cgcgacgatt tgtgggacga aaccacccgc aacccccgca
    60241 ttcccaagcg cggggacctg acctggttcc cgtacgagta ctgacaaaca acaaggagag
    60301 aacatgagca gaaaaacagc ctggttcatc gcatgcggca tggcggcgct ggccgcgggg
    60361 cccgcctcga cagcgtccat gatctatgaa atcaccacgg acacctacct ggacagcgcg
    60421 tccccgacgt acaactacgg cgcgcggaac gtggaccggg tcgtcatcaa cgcctcgtcc
    60481 gccgcccgta cgctcttcaa gctgccggcg gaggtctggt cctacgcgcc gatccagatc
    60541 gtatcggccg tggtgtcctt ctatgtctgg agcgataacg cgcaaaccta cgacgtgtcg
    60601 ctccatccca tgctccggga cttcgccgag ggcaccggca cgggcagcgc gacgggcgac
    60661 ggcgccacgt ggaacacgta cgacggcgtc aacgcctgga cgacggccgg cggggatttc
    60721 gactccggct attccgtcct cggcgtgaag ggaacgctgg gcgtcaatcc cggcgagccc
    60781 aatggccggt tctatacgtg ggacatcacc gccctgctaa acgatgtcac cgcctggagc
    60841 gaactgcagt cgtacggcgc catgctgcgc atcgacgaga cggtcccgcc cagcggccag
    60901 cggttcaacg cctacaccag ttcggacagc acgacctata ccgccccgta cctgccgagc
    60961 ctgacgatca cggccattcc ggagccgggg accctggcgc tcctggccat gggcctgccg
    61021 gtcggagcga tgctgcttcg ccgccggaaa cagggtattc tctcctgctg attctccccg
    61081 agacaatcaa gcggaatgaa acggcgcgcg gtccagtggg cggcggtggc cctggcggga
    61141 ctcctcgagt ccgccggggc ggccaccgtc cgctggccga tcgcgacgga cacgtacctg
    61201 gacagccgga cggagaatca aaccctcaac tacggcgcgg ccaacacgct gaaggccctg
    61261 atcaacaaca acgtgaccgg cgacggctcc gtttgtcgcg cgctgttcac gctgcctgcg
    61321 gaactgtgga attacagccc gaccagcctc gtctccgtca cggcctggtt ctacgtgtgg
    61381 caggacaaca ccggcgaccg ccacgtcacg ctgttccccc tcgcgcggac gttcgtggaa
    61441 ggcacgcaga acggcggggc ggccgacggc gcgacgtggc tgacgtacga cggcgcccac
    61501 gcctggctgc aggccggtgg cgacttcctg accaatttcc cggccgccgg cgtcaaggaa
    61561 gagatcctgg acgaaggaat gcacgaccgg tttttcagct tcgatctcat gcccctgttg
    61621 accaacgaga ccgcccggga ggcgctccgc cagaacgggg ccttgctgcg gatcgacgag
    61681 cagccgattc caaccaatgg cctgccccgc gcgccgttca ccagctccga cgatccgggg
    61741 tacacggcgg agtacaagcc gcacctccgc gtcgaggtgg cgccgccgga acccgagatc
    61801 ggcgggccga cgatgaacgg cggcacggtc acgcttccga tcgggcaact gacgccggga
    61861 atcacgaaca ccgtcgagcg gtcgtacgac ctggccgcgg cgaattggac ctcgatcgcc
    61921 gtcttcgtga ccggcgggaa cgcgacgaac tggtccctgc cgttgtcgcc gtcgaaccgg
    61981 gtgttctacc gcgtcacgac ccgatagtct taagggaggg cgccgtgttg tcggcgccga
    62041 aaacgaggcg gcgacgacgc gccgccctcc ggtgctccaa ggggcgttgt cacacgcccg
    62101 tcttgagcaa ccggtacagc tcgcgcatct cgcggccggc ggccgtgtcg catcgctcct
    62161 ggatcgaggt atagaggacc aggactttcc gcccgtcgac ggacagccgc gagccgccgc
    62221 cccgggcgcc gcccgagatg gattccacga gggggccgcg gaagaccgcg ttcatttccg
    62281 cgaccatctg ccaggcctgc ttgtacccga tgcccattcg gcgggcggcc tcgctgacgg
    62341 actgcgtttc ggcgatcaac tgcaacaggt gcgccttgcc cggcccgatc gtcgcgcaga
    62401 tcagccgcag gcgcggcctg gcttccggct gcccgaatat ttttttcagc gtgtgtcgtt
    62461 tggtcgtcat ggcgcaccta gaaatttaca gcggatttca gggatatata agcctgcgcc
    62521 cgctcgtccg acggctcgcg gtcgaaggcg tagaccacgc cgaggccggg agcgacatcc
    62581 aggaagcgca ggagcgtcat cgagtaattg agttcgagcc ctacgctgtt gagccagtcg
    62641 ttttccggct ggccgggagg ctcgccgtcc caggccatgc ccaggtccca gaacaactcg
    62701 ccgaagagct gctgccggta cacgggtttc gtcgcgctca tgcccttgta gacgcggcag
    62761 agagggaatc gccaggccgt gatcgcgcgg gtggcctgcc ggccgacctg cgtgttgggc
    62821 tcgtacccgc gcagggcgag ggttcgcgcg aggccgggcg tggagttttc cacgatgtcc
    62881 cccaacccgc ccacgccgaa gaagccctgc gccgtgtcgt cgcccgagcc cgcgccgtag
    62941 atgccgtcca tcttcagcac gtgattctcg ccccagggca gcgtgaagta ttccagccag
    63001 gcgaccagcc accgaacgcg gtcgaggtcg ccgcccagcg cgcggtcggt ccactcgatc
    63061 ccgccggaaa tgtaacgccc gtcctcgacc gagtggctgc gcggaaacgc cgtgccatcg
    63121 aagtattcga gttggccgaa cagcgcggcc tcggcgccct cgaacaggtt cgtggtcagc
    63181 agcgcgcgat cggcatagtt ggtctcgccg gattcgagcg cctcgcgatc aagataacgg
    63241 tagcccagcg tgagggccag ctcgcgatcc gcccggatcc acggcacggt gatcgccagg
    63301 ccggcggcgc cggatttctc ttcgtagtcg tacgggtggc cctccgtgtc gcggacaagg
    63361 tcggggtacg tgtccgccga gtgcagcccg aacgcggtga aaacgggata cattcccgcg
    63421 taggaataca cggccgagcc gacgggtatc tcctcctcgc cgtcgtaccc gcccagcagg
    63481 gagagcgact ggaagccggc cggatcggaa aggcgcgccg ccaggccgcc catcgcggac
    63541 tcctccgtcg ccgcggccca cggcgtccag tagtccaggc gcgcctccag cagcgaacgg
    63601 tacggccggg ccgggccggg ttccggcgcc gccttttcgg cctccttcag ccggcgctgg
    63661 ttttccgcga cggcaggcca ctcgcgccgg atggtgggca gcgccccggc gatcggtttc
    63721 aggttcccgt agtcgagaac cgccaggtaa tagccgtacg aatcgtaggc cgcggcggcg
    63781 agcttcgtcc catccggcga gaaggccggc tcgaagagtc cgccctcgac atgtgtcagg
    63841 atttcgggct ccgcgtccgc cttgaacggc agacggtaga gattgtacac gccgctccgg
    63901 tccgcgctga agaccagttc cttgccgtcg ggatggaacg ccggcccgag gacgatgcac
    63961 ggccactgga gcagcgtgga accctcgccg ctttccaggt ttacgcggcg caattcggat
    64021 tccccttccc gcccctgcac gaagacgagc cactgcccgt ccggcgagta aacgggatcc
    64081 acgagcatgt tgaacggctc cgcctcggcc aggacgcgct cccggtcccg gcggccggcg
    64141 gaggccagcg gaacttcgac cagccggcag accgcggcct cgtcgcggac ggcggccaac
    64201 ttcttcccgt ccgggctgat cgcggggaag cggtagcggc cggccgcgct gaccgcccgc
    64261 gtccggccgc tggcggcgtc catcgcgcgc agttcgctgt acatgcggtc ccggccgcgg
    64321 acgttcaggc gcgtgtagta caaccggtcg cccgcggcgg ccaggcggga aattccggcc
    64381 tggacccggg cgtgtttcag cggctgaatc cggccggact ccaggtcata gcgaaagagc
    64441 acgtcgcgcg ccgcctcgcg gttgccggca aaaaatatgg attgcccgtc agcggagaat
    64501 ttcggctgcg agacgagcag gcggtcgggc gtccgctttt tcaactccgt cagcggcttt
    64561 tcgcgcagct tcgccaactg cttccgctgg tagcgggttt cctcgtccag cgcccggcgc
    64621 gcgaggtcgg cgaaggattc gccggtcacc gggcgggcgc ggtcgtcgaa gaaccagggc
    64681 cacgagcggc tgaccgagtc ggcgagttcg ccgacgacgc tccggtcctt cgggcccgcg
    64741 gcgtcctcct gcgcgcgctg cacggcgcgg gcgccccaga ggtaggcggc gtcaccgtag
    64801 ggccactcgg gatgcgccag ttcccactgc cgggcatcga gcgcgcggcc gtcggcgacc
    64861 gccatgcgca tgaccatgtt ggggaggctc tggcggccgc ggccctggcg ggtgaactcg
    64921 gtctcggacc agatcgaggc tccttcaagg aaccagttcg gcgcggtcag gccgggcggc
    64981 agggcgaagt agaagaggag ggtggagacc gggtcgccga tttccggcag gaagcgcccg
    65041 aacacgcggc cgagccagtt cccgaagccg tgcttcgcgt cgagcatgag cacgtgggtg
    65101 tactcgtgcc agaccgtgct gcgcaggtag ccgcccggct cgtagatgga ggacggcgac
    65161 ggcccggcgg cgaagaagga caggcgcgcg cgcgggatcg tgaaggccca gccgttatgc
    65221 tcgtcccagc cgtcgtggaa gagaacctgc gccttctcgc ggggcgccca gcggaagatc
    65281 ggggagagca cggcatgggc ggcctcgcac tcgcggacca gcgcgggcgc gcggtcggcc
    65341 agggattcct ggtaatagac cacgaagtga gcgctctcga cgcgccggat gcgctcgccg
    65401 atcggcatgt cctgcgcgcg gccggctgcc ggcgctgcaa gcagcagcgc tcccatccag
    65461 aaacacgtgg cggccttcat gatggttcct ccgttcgcgg atattcgacc cgaagcccgg
    65521 cgatccggca agcgcggaat atatttgttt gaacaggccc ggcggcggag ggtacgttta
    65581 aaccccggtt gataaaatca acaaacggag gggtcgatca tgaaacgcat tttcggaacc
    65641 ggcctgttgc ttgtcggact gctgggcctg tgcgccctgg ctgacccgcc cgcggaactg
    65701 gattaccagg gcaagatcct ggtcagcgac atcccgctga cgggcccggg atacttcaag
    65761 tacgcgatcg acgatctcgc gggcacggtc aactactggg cgcacgacgg cacggccagc
    65821 ggcgagccgg cgacctacct gacgaacgac tgctacaacg gcgtcttcaa cgtccgcctc
    65881 ggcgccgcgc cgatgacggc gatcgatccc gatatcttcc agaacggcaa cgacctgtac
    65941 ctgcgcatct ggttcagcga ggaccaggtg accttcaacg aaatgctgcc cgcgcagaag
    66001 ttggtgagcg cgccgtacgc ggtcaactcg gacaggatcg acgggtacga cgcggacgaa
    66061 ctcatcgccg cggcaaccaa tgccttcacg gagaccgatc cggtctacag cagcagcgac
    66121 gccgccggcg tcacggccag cgacatctcg aactggaacg ccgcctacgg ctggggcgat
    66181 cactcctccg tcggctacct gaccgcggaa accgatccga tcttcggcgc gagtgcggcg
    66241 gcgggcatca cggccggcca cgtcctcaat tggaacgccg cgttcggctg gggcgaccac
    66301 gcgctggccg gctacctgac ggcggaggcg gacccggtct ggacggcgtc cagcaacaac
    66361 ttctatacca agggcgaagc ggaatcgcgc tacgtgaacg tgaccgggga cacgatgacg
    66421 gggacgctgg tcatgaacgt cggactgaac aacgacctga tcatcagcag cgcaggccgg
    66481 actatcggga tcggtaacaa caccggcgtc gggatcggca acgatcgtat cgcggtgggt
    66541 cgggaggtca ccaatttcgt ggataatacc gcccgcatcc gcggcacctt ctggatggac
    66601 ggcggcacgg ggctggtcgc ccggcccgtg ttcggcacgg gcggctggaa gacgctcgcg
    66661 ccgatcccgc ctttccagca cgtgatctac gtggccacca acggcacccc gggggggccc
    66721 ggcaatatcg accggccttt cgacaatccc cagaacggct acaactacgc cgccgtaacg
    66781 ttcaccaacg accccgtggc gctggtcatc gccgccggcg cgtacggcgg cctgaacatg
    66841 aacgccggga acatccatat tctcggacaa agccgcgcgc aactggatac gctcaccgtg
    66901 tcgagcccgg cgcggatcat catgggcaag cagcgggtgg agagcctctg cgtgaagaac
    66961 gccgccatcg tcgccgcgga cctcggggag gacgtgaagt tcgacaactg ccggttcgag
    67021 cgcgggctca cgatctacgg gcccgacgtg gaagtgcagg actgctacgc ctccgccatg
    67081 gacgggtcgg ccatcaccgt cggcgacggc gtgaacacca tccagaacgt ggccctgtac
    67141 aattcctcgc tgtacaacga atccggcgtc atgggcacgc tcctggtcaa cccgagcgtg
    67201 ggcaacttcg aggtggtggg ctgccagatc gtcaacgggg ccctgggcat ctgcatagaa
    67261 gaccgggagg tcggcccgat tgtaccggtc cacctgtaca gccacaatgt catccggggc
    67321 atgccgcccg cgccggccag cgtggtcgcc gtgctcgacc agttcgccgc cggcacgacc
    67381 atcgccttcg tgcagaatac cgtgctcggg cacgtgggga tcagcgccca tccccagtat
    67441 tacgccaaca acatcgtgta cggacggatc aacaacatcg gcggggcggg gacgcccggc
    67501 tgggcgcagg ccggcgccgg cgtcggcgcg gatgcctcca acaacaccga gcaccagggt
    67561 gccttccccg tcctgccgtc atcgtggagc gattgaggag ggcgtcatga acaagaccct
    67621 ctccttcctc gccgctttcc tgtccggcct gctgccggcc gtcctgtggg cggccggcgg
    67681 ccgggtcggc ggccacgcgg aaatccagta cgaggccgtc ggcgcgggcg gcgcggcctt
    67741 cgcctcaagc ggcaacgtca agctcggcgg cacgctcggc cagggcggcc tcgccctcct
    67801 cgccacgaac gaccaggacc aggtcttcct gaacgggttc tggaagtccg aggattcctg
    67861 cacgctctac aacccgacca tcacgcagct gacccagagc accggcaccg tggccgtcac
    67921 cttcatggtg atcaacggca atacctactc ggtgcagtac gtgacccatg aagagggcgg
    67981 cctgatgctc ggttcgcacg cgatcacgaa tcccgtgaca acggtcgtcg ggaccgggct
    68041 ggcgggttcg acgaccacga tctggcacaa cgtgagcgcg tctacgaacc gcgtccggtt
    68101 ctacgtcatc cgctgcgagt agccttcccg cgccggcggg acgtcagccg tctatggcgc
    68161 agtgctttac cccttgaacc gcaggccgtc tccctgtaca ttgcgggctc cgcaaaaaaa
    68221 gcaactcggg gaaggggtat ttcgatggcc ggcattttca aggcgtacga tattcgggga
    68281 atttacggca acacgctgac ggaggacacg gcctatcgca tcgggcgggc gttcgccacg
    68341 ttcctgaaat gccgcaaggt cgtcgtgggc cgcgatatgc ggccgcattc ccccccgctc
    68401 ttccaggcgc tagcgcgggg cctgaccgaa cagggcgcgg acgtggtgga catcgggatg
    68461 tgctcgacgc cgatgtccta tttcgcgaac aaccggctcg gggcggacgc cagcatcatg
    68521 atcaccgcct cgcacaaccc cggcgagtgg aacgggttca aggtctgccg ggagaaagcg
    68581 atcccgatca gcggcgagac cggcatcggc gacatcgagc ggatattcca ctccggcgcg
    68641 tttgccccgg ccgccgcgcg gcccggcgcg atcagcaccc acgagatcct cgacgagtac
    68701 gccgccacca tccgcgggtt cgccgagatc cgccggccgc tcaaggtcgt ggtggactac
    68761 gccaacggca tgggcatcct cgaaggcaag gtcctggacg gcctgatgga gatcgagccg
    68821 ctgttcgaca ccctcgacgg caccttcccg aaccacgagg ccaacccgct caagcacgac
    68881 accctcgagg cgctccaggc ccggatacgc tcc
//
LOCUS       DAOOTK010000004        63498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_363_length_63498_cov_6.568668, whole genome shotgun sequence.
ACCESSION   DAOOTK010000004 DAOOTK010000000
VERSION     DAOOTK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 63498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63498
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_363_length_63498_cov_6.568668"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="PK634_01100"
     tRNA            complement(1..77)
                     /locus_tag="PK634_01100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Val,seq:gac)
     gene            complement(68..1249)
                     /gene="mutY"
                     /locus_tag="PK634_01105"
     CDS             complement(68..1249)
                     /gene="mutY"
                     /locus_tag="PK634_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOW96363.1"
                     /translation="MKSAPPPGPSLSPALKRAFRVRLPAWFEKRRRPFPWRTRRTPYR
                     VWISEIMLQQTRADQALPYFRAFVRRFPTLRSLAAAPQREVLLAWEGLGYYARARNLH
                     QAARIIARRHGGRFPSDPAALRALPGIGEYTAAAIGSLAFGHEAPVLDGNVIRVLARV
                     MALEDRIHTRAATQKLAAWAEALKIPGRAGLSNEAQMELGALCCTPRDPDCAACPLGN
                     VCRARAGGKPEAYPVKKRRAPVPHKVVGAAVTLRRDGRILVAQRKDSSMLGGLWEFPG
                     GGLDPGETMDQCIARELSEEMGVRLEVGPLLTIVHHAYSHFTIELHAHWARIRAGRPR
                     AIHCADFAWVRPGQFGRYPFSRADHHIIEALRAPAGRAARKELISMWFGGKKKPVLKG
                     V"
     gene            complement(1246..1764)
                     /locus_tag="PK634_01110"
     CDS             complement(1246..1764)
                     /locus_tag="PK634_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96364.1"
                     /translation="MNTKRYRESRAGLIWLGCALLAAAGCGDDPAIPFGPVDEAVLQE
                     PGPQDRVLEGAEEETRALRLAREARASDGEGTNEDWVKRQMELAEQDVLFPRWEARRT
                     AAGKYEVTFTYTVVGIGDVVARRGFAWTADVVLMLVTPPREMQAEELVPRSSRVYQRR
                     ALERRPVELSPE"
     gene            1902..2834
                     /locus_tag="PK634_01115"
     CDS             1902..2834
                     /locus_tag="PK634_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96365.1"
                     /translation="MAEQVPDSKPDEASVPPRIKLNGNGKTEAPPRISLQPKQPGKSE
                     TSRIELGEARPADPMELAKKMTARIDAPKPPVRVISEVERLATAKTATQQFKSMTQQV
                     KSATAPISKAAIPDLDAAKKQTARIDLQEVLGSGDEDIFKRRTAILDPSKIQAAEAAA
                     QPKTIRIKRPEGGTGPIKPVSEPMKPAEPVAAEARKSETARIELPADVAEEAAAEAPT
                     RRKTIRIKRPEGGGAPMAIRPMSVSQPATAVTRAEPGELPVIAPAEEGPGAVYSILAI
                     AALLVACVLIYVLAAQVGTVESLTPPHMPFPGGV"
     gene            2855..2998
                     /locus_tag="PK634_01120"
     CDS             2855..2998
                     /locus_tag="PK634_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96366.1"
                     /translation="MKLEDLAYAIAQKVFWELEHTHHFVVPEAVRKEILAAVRAQLHS
                     LIK"
     gene            3017..3349
                     /gene="trxA"
                     /locus_tag="PK634_01125"
     CDS             3017..3349
                     /gene="trxA"
                     /locus_tag="PK634_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOW96367.1"
                     /translation="MTAAGIVHVNAQNWKIEVADSPVPVLVDFWAEWCGPCRVIAPVL
                     DELSGELASRLKIAKVNVDESPELAGDYGIRSIPTLLLVKGGVVQQQMVGAMSKAALK
                     TRLESELD"
     gene            complement(3350..3838)
                     /locus_tag="PK634_01130"
     CDS             complement(3350..3838)
                     /locus_tag="PK634_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOW96368.1"
                     /translation="MKLLLIRHAAAAEPEDFGGSDLDRPLTDEGRKKARRAFKTLAVL
                     HPDVQLILTSRARRAIETGALLADCLGNPAQVASERLNPGCGAEDLVRALAEWPALPE
                     AVAVVGHEPDLSHILGHVAGAGALRVRFKKAACAVAEVNRQLKGELQALLPPAALCAK
                     DA"
     gene            3903..3976
                     /locus_tag="PK634_01135"
     tRNA            3903..3976
                     /locus_tag="PK634_01135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3935..3937,aa:Gln,seq:ctg)
     gene            complement(3995..5089)
                     /gene="dnaN"
                     /locus_tag="PK634_01140"
     CDS             complement(3995..5089)
                     /gene="dnaN"
                     /locus_tag="PK634_01140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOW96369.1"
                     /translation="MKLKIAKDALLEGLQKVQPVVSTRTTLPILQNILFKADKEKLWL
                     SATDLDLSVRAGVAAEVSKAGATTLPARRCAGIFKELPAGDIEIEADDKDQTAIRSGS
                     AFFKIIGISEEEFPPLPKFDGGKTYTLDQRVFRDMLLNVGYAASTDESRLTLNGVLLS
                     FKGDKLSVVATDGRRMALYEQEVEFPKDAEAELIVPAKTVAELSRTLGAEGSLKIHAV
                     NKQAAFEFNDLLVVSKLMEGSYPNFRQVIPAQSEERVKIEREALMTVVRRVALIASEG
                     SNSVRLTFSKNKLEVSATSPEVGEARETLAISYAGKQISVAFNPDFVLDPLKNLVCDE
                     IYLELTDDMSPGVLKCDLPFLYVLMPIRLS"
     gene            complement(5363..6700)
                     /gene="dnaA"
                     /locus_tag="PK634_01145"
     CDS             complement(5363..6700)
                     /gene="dnaA"
                     /locus_tag="PK634_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOW96370.1"
                     /translation="MQASLVWSKTCEHLSETLPKDIFDRWIAVIQPESFSSDTMVLSV
                     ANDFYQTWLEENYLPLIRKAVSAVCGAPVQIQLTVDSSRTIAPARATAPGVERPPRRK
                     PAAGSPNLNAGYTFENFIVGSSNNLGHAAALAVADSPAHAYNPLFLYGGTGLGKTHLM
                     QAIGNHVLPHGKLSVFYTSCEAFTNAYIDALQKKALVDFRKRFRSYDMLLIDDVHFLA
                     GKEGLQEEFFHTFNALYESRKQIVLTCDRPASEIPAIEHRLVTRFEWGMVAELEPPDI
                     ETRIAILRQKQQTCSLQLPDSIVHFIAEIIRSNIRSLEGALTRVVSYASLTQKPVTQE
                     TVEYLLRDKIDQAQKETLSAEAIQRAVADHYDIRLGDMTGRQRSQAIAMPRQVAMYLC
                     RTLTNQSLPVIAERFGRNHATVIYACRQVAERMKTDTALRQTVSVLKQRLTRG"
     gene            7251..8375
                     /locus_tag="PK634_01150"
     CDS             7251..8375
                     /locus_tag="PK634_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOW96371.1"
                     /translation="MKKLTGPLLIAGSPEKVPDLLYATGFSAVDPVVYLQDRGRRWLV
                     VPLLEAGRARRQAKKGVEILIPAELVRRRSAQRRRDQWILALLRRARARRVTVPGYFP
                     VDLVRRLERRGIRVRVAPGSVFPEREVKDAAELRRLRLAQRAAIRAMRRAVGLLRRAR
                     ARADGLLELEGRVLTSERVRHEIDTTLLEHDCIATETIAAGGTQGADPHERGRGPLRA
                     GEPIVIDIFPRRRADGYWGDLTRTVVRGQASPAVRKMHEAVRRAQRAALRGIRAGAAV
                     APIHRAAQRALEAAGFATAGRGAEAEGFIHNTGHGVGLEIHEGPIVGDAPGRLRAGHV
                     ITVEPGLYYRRHGGVRIEDTIVVTAKGSRPLARCPDYFEL"
     gene            8378..9226
                     /locus_tag="PK634_01155"
     CDS             8378..9226
                     /locus_tag="PK634_01155"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOW96372.1"
                     /translation="MDEWLSFLARGGPFVLVPAALAAGFWLFEWTKIYRPSRLLDTSP
                     AAVQLDYEDVDFVAEDGRPLAGWWIPHPRARGTMLHCPGQSGNMGDRLHLAVDYHQLG
                     MNVFLFDYRGYGRSRGIPTEKGTYRDARAAFEVIRARYGDAEAPPVVVHGVSLGGAVA
                     VQLALDKPVRGLILEGAFTSIRDMGARLYPWLPMRWLSRFRYDSLAKMPRLKTPLLLA
                     HSREDERTPFEFGRRLYEAAAEPKRMVELTGRHRDAGWNRTPEYGRALQDFLDRVLGP
                     LPRDNN"
     gene            9282..9893
                     /locus_tag="PK634_01160"
     CDS             9282..9893
                     /locus_tag="PK634_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOW96373.1"
                     /translation="MKNGLRYWGAALLAGAAIAAQAQGPMGGPGMPGCAMQGGGGCPM
                     AGGGREQALKAAGATDEQVARVREKMYETRKQMIALRAEQELARMEVQHLLSQDKVDR
                     EAVMKAVDKTGAVETSIHKLRVQQQLDLRDIVGADVLKKARESAFEHRRDKQDGRRED
                     RRGDRWGGRGEGKRGEGEGNRGRFQPPPPLEPEEIPAEEPLVE"
     gene            complement(9941..10588)
                     /locus_tag="PK634_01165"
     CDS             complement(9941..10588)
                     /locus_tag="PK634_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOW96374.1"
                     /translation="MDPAGQDKSELTLSIAPGVTVRLVRVAALDGWVQPMPVTEGQFH
                     GFDAHIPNTGRPLTGAPWDRARAFCSWMNAQFDAEAVAPGHVFRLLRQWEWTSCARGE
                     RSDGPAWGDRCPGLGCLEVVENLPLEHRVDDSPNAWNLCALENGLWEWTEDIFDPDMN
                     RRIIRGACWFGTHEGERQFLFFRTPAPHRSHPGFGFRLMIGRPIPGSHPTPSGEH"
     gene            10686..10761
                     /locus_tag="PK634_01170"
     tRNA            10686..10761
                     /locus_tag="PK634_01170"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10720..10722,aa:Glu,seq:ctc)
     gene            complement(10806..12416)
                     /locus_tag="PK634_01175"
     CDS             complement(10806..12416)
                     /locus_tag="PK634_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOW96375.1"
                     /translation="MALTTEIARRRTFAIISHPDAGKTTLTEKLLLYGGAIHLAGSVR
                     ARRNQRSTTSDWMELEKERGISISSTLLQFEYGGCVINLLDTPGHKDFSEDTYRVLTA
                     VDSVIMVIDAAKGIEERTRKLFEICRMRGIPIFTYVNKMDRPSRNPLALLDELEKVLG
                     IGAFPVTWPLGSGDEFCGVYDRLEHNLHLFERTAHGQHRAPTRVSGIRDESIRGQIPA
                     RVYDPWLEELEMLAGAGEAWDEEHVMAGLITPVFFGSAMNNFGVELLLHYFVQHGAPP
                     QPREVGHGKIEPTHPEFSGFVFKVQANMNPKHRDRLTFVRVCSGLFVKDMVVADPETG
                     KPIRLSYPQKLFGQDRESLEIAYPGDIVGLVSHRAFRIGDTLTTDPAIRYDEIPRFPP
                     EAFAYLRNSGTAKYKQFRAGLDQLLQEGVIQSFTSSDVPQNTALLGAVGELQFQVVMY
                     RLQNEYGAEPVMEPAPFAQIRWFAPGADTGALEGGFLGSGVKLARDVRGQTVILFPNT
                     WALEYFVKEHPGMELHAVSPHDLAPARA"
     gene            12613..13029
                     /locus_tag="PK634_01180"
     CDS             12613..13029
                     /locus_tag="PK634_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96376.1"
                     /translation="MGGMVYASRAYGDGRILFGPGCTISLDMDTGFMDAGSRVGFSVI
                     GTGDSRTYGDISVYFTGGGANDIVSQGSWYWVTTCDTGIPFDSKGLHVEIDIQETNNF
                     TCRLTPAARTPAPAARTTPSSIISTCCPRRASPTSR"
     gene            12993..13232
                     /locus_tag="PK634_01185"
     CDS             12993..13232
                     /locus_tag="PK634_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96377.1"
                     /translation="MLPPPRITDFALTDGTNAAVRFLPRPELKNAVQACNDLSAGNWV
                     PVATNLTGTGLDALQVNDAVAADVASRYYRIRAAP"
     gene            complement(14006..16438)
                     /locus_tag="PK634_01190"
     CDS             complement(14006..16438)
                     /locus_tag="PK634_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96378.1"
                     /translation="MKPFRTAIGLATLLLASAAWAATPARWISRGPGGGGALFGPSIS
                     PHDPNDLFVSCDMGLAFRSADFGQSWVTLDFRQTSGHNRMTWVQFTSDPNVLYGLSYY
                     SPMKSDDGGRTWHEILLDPWTRESYVVFADPQRTNRVLTADYTDVYFSSQGGTNYSLV
                     FRTNDNHVAGAFFDGSNIYVGTRAGMIVSTNGGITFAKPATAGIPAGQGILSFAGARQ
                     DGTVRLFCVTMDSGSIWPGITGSEYYGYLGLYALTVGPGAAWTSVVTGIAAGDYPFFV
                     SMCRTNINIAYVAGGSDDGAPIVYKTTNSGGSWQKVFFASGNQNIATGWQGDDPGPWN
                     WMKWSYGEYALGFNVCPTDPNRAAISDLGFVHVTTNGGVNWSQAYAQPSDQNATNSPS
                     DKRKSYLGIGLEDTSCWWLTWAAPNDLYACFTDMRGVASTNGGAAWYFPTGLTYNSTY
                     QSVRRPSSGVLYAAASSVHDLYAWDAYCQDSRIDSGGGAIIYSDNNGRGWKMLHDFAH
                     PVVGLALDPNNDRRMIASVVHSTLGGIFLTTNLQAGTGSTWTKLANPTRTEGHPYVVH
                     ILNDGTLVCSYSARISGDFTASSGVFVSTNAGSSWLDRSAAGMQYYTKDVVPDPHDPA
                     QRTWYAGVWGEWGNSEGLGGLYRTTNRGLAWTRITTNIDQAGSCTIHPADSDQVYVTT
                     ENQGLWVSTNLRAATPSFTALTNYPFRFPSRVFFNPHDTNEIWVTSFGNGLRLGRMTE
                     PEPHLTAIQEGPHGATRLTAQGAAGQRIILQGSATLLNPTWLNMATDSVLDSTLTFEA
                     VPPGFYRTAVGP"
     gene            complement(16500..17711)
                     /locus_tag="PK634_01195"
     CDS             complement(16500..17711)
                     /locus_tag="PK634_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96379.1"
                     /translation="MIIGRPEILRKDGEAECRAAVEWSKGAGVLWYRVPAEHGDWLSE
                     LSDAALVALLIPAMDAGEDIRLDGAVSARLLYALNGCYQAVLRGLMPFLRPVAIRAEA
                     THSDSPRAPGVATAFSGGIDSFCVLADHHYARPPEGFRITHLLFSRTGAFGEAGDRVM
                     WERCERLRGHAERMGLPLVRVNSNVAEFYRPGLGNLLTHTQRNVSVGHALQGGIGRFL
                     YAAGFSYADLRLDPTYTMAHADAVTLPLLSTDRLDTISAGGEYTRVEKTLKVAGIPDT
                     WTSLNVCAGLASAGNCSTCWKCLRTLLTLDIGGVLDRYAAVFDLAAYRRRRNSFIGKV
                     WSSRESLHREIVGLARQRNWRRPLSSRFYRWLWLPLNRLNELALRRGPDLPAWLKAAR
                     RLDKALSRPSS"
     gene            17822..18658
                     /locus_tag="PK634_01200"
     CDS             17822..18658
                     /locus_tag="PK634_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96380.1"
                     /translation="METLRLHMTFGLIQQIRLVHALAESSHPVSIPASEEPKFDALRA
                     RFGFRFAPPDAPGVDVTRFVRCEHRAPATFIGRIERPVIFPRAMADYCRSLWPARRAH
                     RFSFQGLVFGARRTLLQKWIKANLGGRTPRLPDAEGAVARWRNKLFRAAGLDPTRKQR
                     IGDLLLWESSRGRRFPVKAWDEEYVGALAASEFVLCPDGEHVWTYRLFESALCGAIPI
                     VERACPAYAGFRFRSFEDRAGRLTWSAEDAEHNYRLCAERLTIAQPTLDAEIAHILEQ
                     AR"
     gene            complement(18689..19786)
                     /locus_tag="PK634_01205"
     CDS             complement(18689..19786)
                     /locus_tag="PK634_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96381.1"
                     /translation="MNRGLAFLAWVALAAAAAGRDAPLVIDHACADLWQIPDYWLDQA
                     RAQFRIGYGHTSHGSQLVSGMENFRGVEGSRYYYTSASWGLNPGYFLNDEWGNAGGAG
                     DLGHSGDLSWRDATTAMLAEPGNDRNVVMWSWCGGVSDNDEDGINAYLQAMHALEQQY
                     TNILFIYMTGHLDGTGTNGNLHVRNNQIRAFCRANNKILFDFADIESYNPDGAEFLSR
                     FATDGCEYDTNGDENPWGDGNWAIEWAAAHPAHWLNVGDSSCGDCGCAHSVYLNCNRK
                     GAAFWWMMARLAGWPGGPEGGQIQCAGAQVRVEWSSLCTGAVYEVLGAGSLAPTASWS
                     HVASFTNRAGHTNTCALSPGSDRYFYRLKKQ"
     gene            complement(19834..20754)
                     /locus_tag="PK634_01210"
     CDS             complement(19834..20754)
                     /locus_tag="PK634_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96382.1"
                     /translation="MAAILVAALGGARALCAQETAAAPRVVSEGLLEARELDEVSGLA
                     PSLRQPGLFWALTDSANPPELFAVGPDGSHRAVFTVVGVTNRDWEDIDSFTMGGAACL
                     LIADTGDNKAVHPDSSLHIVKEPAVPPGLAAVRGAVPVWRTLRFTYEGGPRDCEAVAV
                     DPAGETVYLLTKRDHPPLLYALPLAPTGVETVLTARRVADAPRIPQPTAEDLAEDRKN
                     GRFRSQVTAMDFAPDGSAAAALTYKHAYLFRRAPGEDWAAAIQREPEPILLPRLRQAE
                     SLCYAADGSALYVTSEQRPSPLLRVELAKP"
     gene            complement(20789..21097)
                     /locus_tag="PK634_01215"
     CDS             complement(20789..21097)
                     /locus_tag="PK634_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HOW96383.1"
                     /translation="MEEKSFLETLFDLSFTEFVTTRLIKVVYVIGIIGAALAGLQRVY
                     LAFRFSGFGSGLLSLVVTPILFLVVVLLIRIWCEMIIAVFRIAENTGRLAEAQKSKAP
                     "
     gene            21284..24124
                     /locus_tag="PK634_01220"
     CDS             21284..24124
                     /locus_tag="PK634_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW96384.1"
                     /translation="MARRAWWIAWIGLAAGVFAAESPPLRPELDYIPGDVPTPWLRYQ
                     NPAARLVEVCGSWDQWAKRHALELKGGVWTLDTRVLPGRYGQYTYKFVVNGDWEKGAD
                     RTLFLNGAGEMEKQSDAIFDATVEGRREIRVRFRDELPENPALTVRLSDGNPVKEWSL
                     VTTREKGSLQGYFISGSTITFVFDEKAYFRKLTRSDKVVVAGNFTWWDGSGGNGRWLL
                     KPTSAGTREMTTQLDGLRAPPGESDLLFKFVINEQEWLPPAAGAPNAVTDANGTENLR
                     VDATLLGGTKLKIITERPVDLSRTTAVILEGLGERPVWRLLTPGGVFDTFVSDKPLGA
                     NLDREHGCTTYRLFAPRASQVHLNLYEGPKFEVYKPAFKRLDPAERYPMWKDPEDGVW
                     EISLQGLDTGRYYSFNIDGPMGNGEGFNVWANVGDPYARAVAHAENNAIVIDPDATNE
                     WFGGWTDQDWRTPAPQELIIYEAHVRDLTIHPSSGVPRDLAATFAGIPATAGTGTGLD
                     HMQRLGANVLEMMPVNEFNNGTRDYSWGYSTVYFFAPEASYGRAPLEGSQYYEFKSMV
                     NELHRRGFAVILDLVYNHVGYPNLFSLIDKKYYFRLDPDFQYSNFSKCGNDVRTENLM
                     VRRFLVDNVVYWMKEHHVDGFRLDLAELIDMDTMYAMAEAARTVNPNALLISEPWSLR
                     GENKYQLRGTPWAAWNNEFRYAAKDFALGRYNRDWLQKKIVGSVDTWSAHPMQSINYI
                     ESHDDMALVDEFSSRPDKDGRNLDEKDVAANRLAATIVFTSLGIPMINAGQEFLRSKR
                     GISNTFDRGDDVNAVRWTDREQPMAAEALAYYEGLMRLRRSPEGAAFRVSSRPPAGYY
                     RWITPDNPQSLGYIVNAPRRHEGAGFIVLLNAGGEPVPFDVPFPQGRWRLVGDGLRLD
                     PKGLPDTGVREGDQSYTVNVPGQRALIFMDGF"
     gene            24150..26165
                     /locus_tag="PK634_01225"
     CDS             24150..26165
                     /locus_tag="PK634_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOW96385.1"
                     /translation="MKKTIPPGGRASPRGIRYRGFFRELDLASIRPAGWLRRWLERQR
                     DGLTGHLEHAGYPFNTPGWRQDPIRWKGRAIRSWWPYEQVAYWVDGMTRLGLLLEDAR
                     LLRKASRQLDRVLARPDPDGYLGPQALKRTTPQGRAERWPHAVLFRALMAGLPAGRRR
                     PILRQLTRHYLSGTAKFSEWRNVCNVEIMLWLHGQTGDRRLLDLARRSLAGFHRRRPP
                     APASLRAMLSGARARDHGVNYCEISKLPAVLFLHTGNRTALRATVNAWRKLARDHALA
                     DGVPSSTEHFRGRAAHAGHETCVIADAAWSLGYLLMATGDAAYADWIENTVFNAAPGA
                     TMPDFKALQYFSSPNQIVADASANHHEHGFGWAHKSYRPNPATECCPGNVTRIVPNFA
                     ARMWMERPGRGLAATFYGPSAVTHRGCTISEETDYPFGESIRFTVRTRRPSTFSLFLR
                     IPGWCRRPRLRINGEPFAGALKRGTFVEVRRTFRDGDRLELELPMDIRRERQPGGGLC
                     IRRGPLLYALPIPARRRRDRHDKRSSDEFPAWNLRPAGPWNYALPKFSNVWKKAEVIF
                     PNIGKKQAEFSKHWKKRAEIFQCLENPWAEPPIAIRVPAVRVPGWRARRLKVLRDKWH
                     GARRGDFRMTPPLPSARTLARASRKVEWITLVPYGCTQLRIAIFPAT"
     gene            26162..27697
                     /locus_tag="PK634_01230"
     CDS             26162..27697
                     /locus_tag="PK634_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOW96386.1"
                     /translation="MKTDKELYRQLRGVPRDRLWTEEVPRFNSASPRERMARVAVIRA
                     VGAAFASSGTTAQRAEVRSWLVGLLRDPSEKIRRYAMTALPKLGAGPGEEAELLSLLR
                     TTGVERERKFLGRTLDKIGGRATLEAVKAAGGLLPQTEQKVKARVARRQSPGRVRMDC
                     RLERFEGLRIHLRCRRGLETIVRDEAGERLVTPGRFRGIEIRRGLVVLAPAAPFSLAD
                     LYALRCFDAVNFVLGTAPAAGGAEYIEALASAIASPLSRRLLSAFTEGAPRYRLEFIA
                     RGHQRGAVRLVANRAYALCPDILNDPRRATWAVDIHPQGRGSVVELRPRLTPDPRFIY
                     RRDDVAAASHPPLAACMARLAGRAEGEIVWDPFCGSGLELIERALRGGVRQVYGTDVS
                     PEALAIARENFAAAKLESVPARFTCCDFRESSAVEWLGPDSVTLIITNPPMGRRIRIP
                     DLRGLFSDFFRVAGRALKPGGRLVFVNPLRLGPADAALQLQFRQTVDLGGFDARLEVY
                     RKS"
     gene            27757..30171
                     /locus_tag="PK634_01235"
     CDS             27757..30171
                     /locus_tag="PK634_01235"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HOW96387.1"
                     /translation="MRAIHAGLVVGMLAAGVTARAQSVTPLFLGGLQPGVTPVHAIAV
                     DTNRGQVLVGGRFSYLSGAPAAGLGILNMDGSVSTVYVSPPPPTGTILAAAFDAPRGR
                     YFFAGSISNPAADRAYLGVAYVTQGVGRVEYWAGTEPGGHIRAMAQHGNHLWVGGAFT
                     SFAGVARQNLAQLSALTGEPTDFNLPASSTVTVFSVLGDDTLLVGGAFTNIGTEPRQG
                     LARLHPSTGALDTNYAGRFQGGPVYAIAPARSNVYVGGLFTSVANQACGRIAKLDALT
                     GARDLTATGGAVGVGAIYTIVPAGNDHLLVGGSFLNYSGNGQRYLALVGTNLDPIAAF
                     TAGVNGIVHAAAALSSTSALVSGRFTQYQDHPALGLVEILSESWRRHPIFSGSVGGPG
                     EVYALAELPDGTVAVGGTFYDIGGEPTRNLARLYPNGTLCQAFVTNGPNGRVYALAPA
                     VHGADTQRVFYAGGAFTSVGDRAVGRLTRYEFTAVAGQYVLTTSVLPHCDGDVRALAT
                     STSHLYAGGSFESVAGITNHEHMMGCVHGSLAPDPAFASQPGSVLVAVLVDTNLFLGG
                     SIYNEEAMWFDGFVRKVSAADGAPTAGFNAPTPNAPVGTLWLSDTSLYAGGDFTIIGP
                     YSANYLARFHTGTGARDATYAMHADASVYALAGTTQSLYAGGYFTSFNTTSRLRVARF
                     DAASGQLQSGYSNIFDGQVNALLSQGSRLWAGGQFSAAGGQPAASLALLGWPQLAYLR
                     QTPAGFELAIAGAPGQTGTVEQAADLNAQDWSAHGSYALGPDENQVVAVVTNPAANRF
                     YRARGE"
     gene            complement(30176..30826)
                     /locus_tag="PK634_01240"
     CDS             complement(30176..30826)
                     /locus_tag="PK634_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HOW96388.1"
                     /translation="MNERSKAGRRFGCLQVLLIVLAAVVLTVAISFLVFRAIYFPSAF
                     RPVTLNAREEQALEAKLERLDFTATNAAAAALEPEPYSEEGAERTIRITEKELNALLA
                     KNTDLADKLAIDLSENLVSAKLLIPLDEDFPVFGGRTLRVKAGLKIRLSGEKPEVVFQ
                     GLSVMGIPLPAAWLGGMKDVNLLDEAGGDQDFWKTFAAGIEDLRVEDGRIAIRLRK"
     gene            complement(30859..31938)
                     /locus_tag="PK634_01245"
     CDS             complement(30859..31938)
                     /locus_tag="PK634_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96389.1"
                     /translation="MKRAIHQLVAGFSYGDAISNEALVLRGIFRSWGFDSDIFCEARR
                     ILPELRGEARDVAGAKESFRPDDIAFLHLSIGSPVNDLFAELPCHKALLYHNITPPDY
                     FRGIQEQIAHHLAKGREQAKRLAGAASVVLADSRFNADELESWGYGPAGVLPLVLDLA
                     RLRGEADRGVLKKYDDGLVNVLFVGRCVPNKRLEDGLAAFHYFQRYVEPASRFIHAGS
                     FAGVEQYHALLLTMMRDLRLQNVELIGSVRQDELNACYKSARLFLCMSEHEGFCIPLI
                     ESLVHDVPVLAYAAAAVPGTLDGAGVLFREKRFDLVAEMMGRLVRDASLRGAVLKGQR
                     ERLARYEQRDLAAELRNHLASLLAG"
     gene            complement(32180..33358)
                     /locus_tag="PK634_01250"
     CDS             complement(32180..33358)
                     /locus_tag="PK634_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96390.1"
                     /translation="MARAQSIAVVSPRFSEAGTVGGAETLLKRLAELAADDGRQVTIL
                     TTCATSHFTWENTVPPGARRVGNLDVRFFPVDADRDVAAFLRIQQAISQRGAFTPEDE
                     RAWIRNSVNSRALLDHLRNTDYDRILAGPYLFGLSFFSAQVHPRKTILVPCLHDEPFA
                     YLEIMKELFGAVGGFAFNTEPERDLARRLFHLPGEKGAVVGMDIEPFEADPAAFARAH
                     GLDRPYVIYSGRREGGKGTPLLCEYLHAFRERTGEDVRLVFTGSGPIEAPAELEPYIL
                     DVGFVSDREKREAMAGAVAFIHPSVNESLGIVLLEAWLAGAPALVHAGGEVLRWQCRR
                     SNGGLWFRHYPDFEQELLLLLRRPELRQRMGAAGRSYVLREYSRTAIQARFLAVLDGG
                     "
     gene            34178..35353
                     /locus_tag="PK634_01255"
     CDS             34178..35353
                     /locus_tag="PK634_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96391.1"
                     /translation="MDDFLVFENVSKSFGKVRAAADVSLTIRRGEFFSLLGPSGCGKT
                     TLLRILAGFESPDSGRVLLNGRDITQLAPNERKVNTIFQSYALFPHLTVAQNIAFGPQ
                     IAGWPRAQIREEVDKMLHLVQLTDQAHKRPDLLSGGQKQRVAIARALINKPQVLLLDE
                     PLAALDLKLRQRMLIELDLIHDEVGITFVYVTHDQGEAMSLSDRIAVMDKGRIEQIGT
                     PAQIYEAPRTSFVAAFIGDTNFFEGQVVELLGGDYSRLTVEGFPSVECFNDNKLNVGD
                     LVYLSIRPEKFRISRERPEPAPHVNAVQGRVADVIYLGSLTKYWVRVQDYRIAVWRPA
                     SRFLLDENPITWNDEVWISWHGDDSFMLERYSESDEKLMEAPPEQVGDPNGHGAAKP"
     gene            35350..36228
                     /locus_tag="PK634_01260"
     CDS             35350..36228
                     /locus_tag="PK634_01260"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96392.1"
                     /translation="MTPARRARWTEALVTTPSLAWLLVFFVVPTVIIFAITFKPVDLY
                     GGIGKGWTLDTLKSLFNPNYPAIVWRTVWLSLATTAVCLILAVPCGYYVARSSDRWRQ
                     MLLLLIILPFWTSFLVRVFAWKVLLHPEGPLKHALAAVGLIGPDTLLLYNEKAILLVM
                     VYTFLPFAILPIYAAAEKFDFRLLEAARDLGARPLRAFWSVFVPGIRRGLLTAVLVVF
                     IPALGSYVIPDIVGGPNSEMIGNKIAQRTFSDRNLPHASGLSAFLTLGVLLPLLLVLA
                     LQRKKGEEAPSMQEVS"
     gene            36225..37001
                     /locus_tag="PK634_01265"
     CDS             36225..37001
                     /locus_tag="PK634_01265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96393.1"
                     /translation="MKRSVIPLLTTLFVLVFFYLPIVVLVVNSFNASRFGGTWEGSTL
                     EWYRMLFRETSVWRALRNTLIIAVGATFASVVLGTTAAFALHRYHTRLQRLHYTLIYT
                     PLVVPEILMGMSLLLFFVAAGFELGLFTIFLAHTTFCISYVAMVVLARLQDFDFSIIE
                     AAQDLGAGWWTTAWRVLLPLLAPGLAAGGLLAFTLSIDDFVITFFVSGPGSTTLPVRI
                     YSMIKHSKNMPMINALSAILLVVTFAAVWGSQRLTKESHA"
     gene            36998..38032
                     /locus_tag="PK634_01270"
     CDS             36998..38032
                     /locus_tag="PK634_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOW96394.1"
                     /translation="MNARILIPALALGATLLAGCGDKTPTLHFYTWADYIKPELVQRF
                     EQEHGCRVIIDTFDSNEAMYAKLKAGATGYDLLNPSSYMVKLLKDQGLLQDLDRSLLP
                     NLANIDPEYLRIAMDPEMKHSVPYMLTNTGIGYLASRVPDFDPSWGVFDRSDLRGRMT
                     MLNDMRETLGGALKFLGYSLNSTNEYELAAARDVVIRWKKNLAKFENEQYKTGLASGE
                     FTLVHGYNGDILQVMEANEDIAFAVPKEGTSISCDDLVIPTTAKEVAHAHAFINFLHK
                     PDVAAENTEFIGYLCPNSAAYPLMSEEIRNDPSIFLAPEIKAKSEVIGDVGESLALYT
                     KMWDQVKAAE"
     gene            38132..39430
                     /locus_tag="PK634_01275"
     CDS             38132..39430
                     /locus_tag="PK634_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOW96395.1"
                     /translation="MSTTHFTLKQSDIPTQWYNILADFAEPLPPPLHPGTKQPVTLQD
                     MLAIFPENLVMQEMCPDRWVEIPAAVRDIYALWRPTPLLRAVRLEQALQTPAHIYYKY
                     EGVSPAGSHKPNTAVAQAYYNKQAGTQRLATETGAGQWGSSLALACKLFGLECVVYMV
                     KVSYHQKPYRKMLMHTWGATVHSSPTRHTEFGRKILDKDPECPGSLGIAISEAIEDTV
                     HHPHTKYSLGSVLNHVCMHQTIVGQECMKQMELAGEEPDVLIGCVGGGSNFAGLVFPY
                     LQHKIAGKKSYRVIAVEPTSCPTLTKGDLKYDFGDTAGMTPLLMMYTLGHDFIPPSIH
                     AGGLRYHGMAPTVSHAKKLGLIDAVAYPQTKIFEAAVQFAQTEGIVPAPESAHAVAAT
                     IDEAVAAREEGKKRVILFNLSGNGMLDLASYDAYLAGHLK"
     gene            39535..40755
                     /locus_tag="PK634_01280"
     CDS             39535..40755
                     /locus_tag="PK634_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOW96396.1"
                     /translation="MPRAALIIFAVTYIGVALGRLPGLALDRTGMALLGGIAMVVAGV
                     LSEREALLAIDFPTLGLLYALMVLSAQLRLGGFYTRVALAVARLMDRPARFLLILMAV
                     SAALSAILVNDIICLAFTPILAVSLLRSGLNPVPFLIGLAVSSNIGSAATIIGNPQNM
                     LIGQVGRLHFGRFLLWCGPPSLLALLAAFLIIAWLYRGRWRRPAPAVNPASDWPAYNP
                     HQAAKGVAATVILVALFFTPIPRELSAIVVAGVLLCSRKMQTRWILDLVDWHLITLFA
                     GLFVVIRGIEAAGLPDALLAALQERGVDIRQPGLLTLVSAALSNLVSNVPATMLLVKF
                     LDPADPVPWYVMALAMTYAGNLLTIGSIANLIVIEQAKPYGIHIGFREHARAGIPVTI
                     ISLLIAIGWIWWRA"
     gene            complement(40816..41643)
                     /locus_tag="PK634_01285"
     CDS             complement(40816..41643)
                     /locus_tag="PK634_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOW96397.1"
                     /translation="MSAEQKITSLQNPRVKDVVKLGRRPHRDEAGLLVVEGYRELRRA
                     LANHWKPNALFYCPPLYLGHHEPELVEACRQAGAELFECAEPVFAKMAYRDRPEGLLV
                     TGPQVRWKLADLKLEKTSPLLVIAESIEKPGNLGTLLRSADSAGADAVLVCDPTTDIN
                     NPNVVRASIGTLFALPVVEASPEEALAWLRARGIRILAATPHAEQDYTQADMTGGTAI
                     VVGSEQYGLKPFWMEHADLQVRIPMLGQADSLNVSAAATILLYEAVRQRRKKSHEIH"
     gene            41711..42379
                     /locus_tag="PK634_01290"
     CDS             41711..42379
                     /locus_tag="PK634_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96398.1"
                     /translation="MKRHAKLLSFLALPLLAAAARADETADFSRYQVIMDRKPFGVAP
                     PVPVAPPPPLTAEQSFARTLRLSGMWQRGDGSVRVAIIDANGNRNLYLSVGEMEEGIE
                     LVSVDQQSEEAVLRKGTEMAVLKISSGEVQPLTQEQQQQRLTQDQAQRMSYAERRKER
                     ERQRQQPPPPPPEPVYTGAELEKHLQEYQMEVIRQGLPPLPLPLTPEMDAQLVSEGVL
                     PPMQ"
     gene            complement(42383..43087)
                     /locus_tag="PK634_01295"
     CDS             complement(42383..43087)
                     /locus_tag="PK634_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96399.1"
                     /translation="MKRTGWRVFGWALALAPLLIFALCGCEVGSADSVVRMVALDFSG
                     FYQGYGSNGTQMVVPPNSGTEVTLLNLRQNGDQLEAIDNNGIVFRGTLGNLVDTTASF
                     TLEGRTTVGQAVTISGTLNGSGTEGTMRGTWIEPSQYSTLSAKAVINPSPTNTEGEVD
                     ISPSSANLTANQQTQTFTASGGTESYTWGLLNTNGTLNTSSGSSVTYTRLTAGDNQIT
                     VSDTAGSSASADITQP"
     gene            complement(43122..43343)
                     /locus_tag="PK634_01300"
     CDS             complement(43122..43343)
                     /locus_tag="PK634_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOW96400.1"
                     /translation="MDTVLLSERIQIERKQFFFDLKENPRGRFLRITEEVGGRRDTII
                     IPSTGLDLFLTTVERAIEADKGRNETASA"
     gene            complement(43502..45493)
                     /locus_tag="PK634_01305"
     CDS             complement(43502..45493)
                     /locus_tag="PK634_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96401.1"
                     /translation="MAAVSLQGFGFNTVAANILVHNGNGVYLGGGQGNLIGGYAAGER
                     NVISSNTDIGMRFAGPGPSNNLMVGNFIGTSPDGLTAWPNRLSGVAIDNANGNTIGGT
                     SAAPQVISGNGTAGISMSGTNTRDNRIVGNLIGTDASGTQKLANAISGIALDGTIGVI
                     VGGTNASERNVISGNGEWGVHLNYGAFSNRLAGNIIGLASHTGWAVSNGFDGVRALNS
                     PSNYIGAAGGGNIIGGSRGGGITVEGTGAVGNVVQGNVIGMGFASNALPNSNVGILVW
                     DNRSNRIGGTAAGEGNTVVYSKSDGIYLRGSNAVGNLVLRNLVGVDAAGTDRGNDGNG
                     VRIDSGISNQVGATLGAGRNVISGNSGAGVYLHGGAQGNMIYGNYIGLNPAGSAAVSN
                     ENTGVHAENAPGTVIGMFIGGNVISGNLLAVNLVYSNQPRGNIDLGGDGPTANDPLDA
                     DAGPNQLQNSPALGQPLIVGGWVYVQGSLTGAPSQAFSIDVYQADGTNFGSRAYIGRV
                     PATTDAGGVGSFASGFTFALATGAALQAVADADSDSIPDYWETLFGLDPAVSNAPTDD
                     ADGDFVPDVEEYVADTPANDPAFYLAITSVTNGGPARYVSFPSKSTRLYEIDGRTTLS
                     ETQTWSSLIAGVPGNDADLTWAESGGDTEVYFRAQASLP"
     gene            complement(46020..47069)
                     /locus_tag="PK634_01310"
     CDS             complement(46020..47069)
                     /locus_tag="PK634_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96402.1"
                     /translation="MMSRILTTAVLLFLWKSASSWAGPLWGRSSAPAQPSAYAAGMLG
                     QVTTLDGYVQETTRPFYDITDPGKNDQFAESYTLEELGFDGGYATFGFSFEKQWRFFT
                     LQANLSYLSPAVRGEAVRDYYIGVEDVSYNGETYDYMHIPEGAPFEAEMQGGSFAMRG
                     LATPVSITAGRAVEITPSLGLGLNTFFSYFEIDAGPAQGVVLYEIPPREYVVGGRGKG
                     WTGMVLPELGAGLDVRFGGGTAEQRAPELLLQAYFALLDFSGSTEDFGISSRNAKDVD
                     LEYDHFEARALVGIPMSKDLELLLGVGVQHMKADAEVMAEDRPAEEVEELREKFNKFI
                     QLEMTTVTFLAGLRF"
     gene            47192..47518
                     /locus_tag="PK634_01315"
     CDS             47192..47518
                     /locus_tag="PK634_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96403.1"
                     /translation="MKMLTINAWLGLLITAVALVVGGFATVTSIRGARGPRERRFVLW
                     HCAGAWTAILLLLGLVVVTPFPWNLAWLGLYFIHLPIVIYRFASKHQLLRVLEARESA
                     RERDVA"
     gene            47585..49495
                     /locus_tag="PK634_01320"
     CDS             47585..49495
                     /locus_tag="PK634_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW96404.1"
                     /translation="MTLRSKTLLVMGASLALLFAAAIGAFFHIMSARDVPEGGLVHLL
                     PWLVWAATAGVLLGILVALLLQKRALSRVARLSAAVLGIQKRADLSARVPVEGDDELS
                     ALALGLNEMLASLERATARRREAEAAQQESEALYRLLFKEMFSGFALHEILCDPDGKP
                     CNYRFLDVNPAFERLTTLRREDVVGKTVLDVLPRTEPAWIERYGRVALTGEPVHFVDY
                     SATLDRTFEVLTYSPQRGRFAVIFNDVTEQKLREQEQAKIQKLESLGILAGGIAHDFN
                     NVLAVVLGNLHLIHMNQPPLDEETRTLLGEAETASLRAKGLTQQLLTFAKGGLPVKQV
                     CELAPVLREAATFALRGSTCRSQLQVAPDLWFVEADPGQIGQVIQNLVINASQAMPGG
                     GVIQIRARNVVVEKGAIHLPEPGRYVAIVVTDTGVGIPPQHLDRLFDPYFTTKQKASG
                     LGLATSLSIVRKHGGRITVESTVNEGSTFRVYLPVTDKTPAPVTDPDVDTLPQGSGRI
                     LVVDDEEPVRRLTSRMLCRIGYEITEAGSGEEAIARFEEARQAGRPFRVAVLDLTIPG
                     GMGGVEILKELRQRDPDVKAIIASGYATDPVMASYREHGFVGVVTKPFRLAELARTIA
                     QAAGAPASPPPK"
     gene            complement(49547..50086)
                     /locus_tag="PK634_01325"
     CDS             complement(49547..50086)
                     /locus_tag="PK634_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96405.1"
                     /translation="MSKWWIVMAVALAAFVPTARAGETFEYPEGDPAFSIRFPGSWTA
                     EVNDQDALEALSGDETVYLVLWEVENLDNLEDEMTATAEQMLSDVEFDDADEELTNKY
                     GVKFWLKHGDGVEKESEAEVQFTIAVFGPSENRAFVVLYTIEKETATDESKRQFVNIM
                     ESISPVEAEEEKESEEAEE"
     gene            complement(50129..51103)
                     /locus_tag="PK634_01330"
     CDS             complement(50129..51103)
                     /locus_tag="PK634_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOW96406.1"
                     /translation="MKAWTRVLRAVMLSAFVGGLVSCSTIDAVTGKKVNNLWSIDDDI
                     ELGTDFYKDIKKWARDEKVGINKDPRRVRQIERVTRNIAAVSHMPDLPYEVILLHTNE
                     VNAMALPGGKIIVLAGLYDPEEGLVRDEDELAVVLAHEIAHVNCRHSTEEMTRNLPWE
                     LLLLGGAIYAEYKEDEDLQDILGGAFLIYEGLIVTKYSRKDEREADRVGLMYMARAGY
                     DPRAAVGLWERAAKDESGIDRALNMLSTHPSCQERARDLEEHMPEALEAYRAAKAAPA
                     GRGKALAQDAGPDWVKIKDPYRSLVSAPADGSDASPTKKIAGQGGWAR"
     gene            complement(51141..52244)
                     /gene="queG"
                     /locus_tag="PK634_01335"
     CDS             complement(51141..52244)
                     /gene="queG"
                     /locus_tag="PK634_01335"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOW96407.1"
                     /translation="MNLSARIRQTAQDLGFDLAGIAPAAPPACRDFLRESAARGYAAD
                     MSWLGRSAARRGQPDAVLPGARSVVALGCSYFTTWPEDALALDPLRGRIAAFAWGPDY
                     HAVLKPRLEELGRFIEREKPGATWRAYADTGPILERHFAGRAGLGFVGRNTQLIHPRM
                     GSMILLGVLLLDQELEYDAPTPGSCAGEEPHPCGRCARCLQACPAGALPEPFRLDARR
                     CLSYLTIEHRGAIPPELRPRLGNWIFGCDECQAACPWVRRFSRPASRPFLRFEPDRWA
                     PRLAELLDLDERAFRERYGGTPLERTGRARLIRNAIVALGNSGRAEARPALERWMSGP
                     DEGLREHALWAARRLDRLDGSASAQENSARPVE"
     gene            52296..54311
                     /locus_tag="PK634_01340"
     CDS             52296..54311
                     /locus_tag="PK634_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOW96408.1"
                     /translation="MNTKRGIAGCADGCGEHAAGPRAWIRGVSCAVSGLLTAGGFAAH
                     AALAGGIPAALAAEASFPALARSFYAAAILAGGWPIVPRALASVRRLKPDMNLLMTIA
                     VLGAVLIGQWFEAATVSFLFALALRLESWSAGRAEKAVHALMDLAPAMARVVDLRDGA
                     MAEQPAESVPAGASVAVRPGERIPLDGLVIDGATTVDPSPITGESAPAAKKAGDPVYA
                     GTINGRGAFTFRVTRPAADTTLAHILRLVEEARANRAPVEQWVDRFARGYTPVVFGLA
                     LAVCLVPPLLGAGPWSAWVYRGLVLLVIACPCALVISTPVSLVSALASAARAGVLVKG
                     GAHLEAAAGIKAVALDKTGTLTEGRPVVQRVIPLDGHTPEELLATAAGLETHSEHPLA
                     QAVLACARRAGVGPRPAVKYQALPGRGAEAEIGGRRFWIGSHRLVHDEGGESESLHRE
                     LLALEDAGHSVIAVGNERHVCGVISVAEGLRPGAAEAVRELRALGVGHVTVLTGDNEG
                     TAAAVSRAIGADDCRAELLPQDKHAAVRELAGRFGAVAMAGDGVNDAPALAAAAVGIA
                     MGAAGSDAALETADVALMTDDLSRLPWLIRHARRTVSVVRQNIAVALGLKILFMALAA
                     AGRATLWMAIAADMGASLLVIFNGLRLLRPARGGEQKPGPPGAFSAE"
     gene            54531..54956
                     /locus_tag="PK634_01345"
     CDS             54531..54956
                     /locus_tag="PK634_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96409.1"
                     /translation="MNRRAFMLVVVLSAAGAAAQGPLADQPPAGSLPPVESNAPAFRY
                     EGFKRIACDSLSNVMLRAAHYFGTNEVLYRVSFADAPTPYFFVRLGDFIGEYEIVGAG
                     GRRGDEHLVLRKGEEEIILPRARKYYASPLTVRREKKRE"
     gene            54953..55807
                     /locus_tag="PK634_01350"
     CDS             54953..55807
                     /locus_tag="PK634_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96410.1"
                     /translation="MSAVAPFYWSPERYATFHACLRRYYYRYYAEAPAFRPARDLRPR
                     TAWADEWLRRGAHEFLRGGLDEEAAAARLLQVLRDDFKASRQHGYRAQPKTTDGLFEH
                     EYAISVPDAEWKDLADRAVADLRAFCRTDYARALAAVPAAARLAVGEFLRFDLLGLPV
                     DIRPDFAARTTNGVALHAWGPPGDGARPLARAALAWLAVGLWKAAPESVEVVEYDFPA
                     GPSAAHRFTAEDLEAARELVHDAAYETRYPLADPDRNAAREQDFDFTDDEATCRACPF
                     LKLCARWR"
     gene            complement(55816..56538)
                     /locus_tag="PK634_01355"
     CDS             complement(55816..56538)
                     /locus_tag="PK634_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOW96411.1"
                     /translation="MTDASPAPVAPGERVAVLFDIDGTLLDMRGAGRRSFAKAVEAVF
                     GWPDDIAYVNFAGNTDLNVLQQVMASHRHAMTERERRTFLARLPEELERAAAGAELVL
                     FPGVRALLETLSGDPRVVLGLVTGNVESCARIKLRQFDLHNHFILGAYGDEHADRGRI
                     AQLALQRVRQALPPGARLRSCWLVGDTPFDIAAAARIGAASVGVATGKFSVEELRAAG
                     ADHVCADLSDTAAVLRRLGLRG"
     gene            complement(56535..57170)
                     /gene="upp"
                     /locus_tag="PK634_01360"
     CDS             complement(56535..57170)
                     /gene="upp"
                     /locus_tag="PK634_01360"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOW96412.1"
                     /translation="MRNVFEIRHPLVQHHLARLRDRDTGPAEFRRLLQRLSALLAYEA
                     TQDLVLQDTTVQTPLAPARGRVVRQRIGVVPILRAGLGMVDPIVNLIPDAEVWHLGFY
                     RDEKTLQPIEYYRKLPQGDPVDVSLVLDPMLATGGSAVAALEAVHAWGVKTVKLLAML
                     ASPEGVRRVHQQFPDMQLYVCAIDERLNEHGFILPGLGDAGDRMFNARAAS"
     gene            complement(57197..57646)
                     /locus_tag="PK634_01365"
     CDS             complement(57197..57646)
                     /locus_tag="PK634_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96413.1"
                     /translation="MEKPLFEIGAAGVDADRLVREIQAEADRKMEQGVYADARIARAE
                     RTNLVNLRNEEQFLGFYLQCLRDAVFVDISDFEIRERRRHLAPLLIRLKKAIWSLLKF
                     YTYRLWSQQNQVNGLLVTAIEGLDEKYAAKIKTLEARIAELEKKTGG"
     gene            57907..58407
                     /locus_tag="PK634_01370"
     CDS             57907..58407
                     /locus_tag="PK634_01370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOW96414.1"
                     /translation="MKTMYDFTVTDIDGAAASMSEYRGKALLLVNVASRCGFTPQYEG
                     LQKLYERYRDAGLVVLGFPANNFMNQEPGSDAEIKEFCSTRFHVTFPMFSKLSVRGDD
                     IHPLYRFLTSEEENPGFGGAISWNFNKFLVGRDGKVAARFGSRAAPESREVMEAVEKA
                     LGSPAP"
     gene            58404..58964
                     /locus_tag="PK634_01375"
     CDS             58404..58964
                     /locus_tag="PK634_01375"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOW96415.1"
                     /translation="MNKTAIFLALAFMALGGPLARGTAMPKFPARVDAEGTPLALRST
                     GIFKARVFFSVYAAALYLEEGVSSARVLEDVPKRLELHYFYSIPASVMVEEGGSALAA
                     NLSPDQRELLRERILQLNAAYRDVKPGERYALTYVPGRGTVLSLNGEEICRIDGADFA
                     AAYFRVWLGEKPSSRALKKALLGPAG"
     gene            59121..61856
                     /gene="ppdK"
                     /locus_tag="PK634_01380"
     CDS             59121..61856
                     /gene="ppdK"
                     /locus_tag="PK634_01380"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOW96416.1"
                     /translation="MAKKYVYSFGGGKADGNESMKNLLGGKGANLAEMAGHPKLRLPV
                     PPGFTITTDVCTHYYANKKTYPKELKAQVAAALATVEKLMGKKFGDVKNPLLLSVRSG
                     ARRSMPGMMDTVLNVGLNDETIQGLIAATKNPRFAYDAYRRLIMMYSDVVMEKAAGIE
                     PKGGKGIRKVLDEHLEHVKESKGYKEDTQLTAEDLQQIVVDFKKTVKDVLGKPFPEDP
                     IAQLWGGIGAVFMSWNGKRAVEYRRIEKIPDEWGTAVNVQSMVFGNMGDDCATGVAFT
                     RNPGNGEAQFYGEYLVNAQGEDVVAGIRTPAPVNEYSKNDQSENLPTLEKLMPRCYKE
                     LFSIQKRLEKHYKDMQDIEFTIEKGTLFMLQCRIGKRNGVAAVRIATEMQKEKLINAQ
                     TAIMRVAPAQLVELLLPMLDPKAELATHAIAKGLPAGPGGAIGRVVFTAADAVEWASR
                     GEKVILVREETSPEDVDGMHKAQAILTSKGGMTSHAALVARGWGKCCVVGCSDMEIAD
                     DDKSFKTRSGTVVKEGEWISLNGTKGLVYAGQLPLVAVDLDHNKSYTDLMKLVDRFRV
                     LKVRTNADTPKDSAQAIKFGAEGIGLFRTEHMFYGEGSDKPLFLLRKMIMSKTLAERR
                     QALDELFPFVKSDMKATMEVMAGRPVTIRLLDPPLHEFVPHDEDKLQALANELKVDMS
                     ELNKRADALRENNPMLGHRGVRLGVTYPEVTEMQVRAILEAAAELIKAGKKAFPEIMI
                     PVTCAVSELDHQKVIVDRVYGEVCRKFGLKKLEYMYGTMIEIPRAALRAGQMAETAEF
                     FSFGTNDLTQMGFGFSRDDIGSFLPDYLNGKLLPDDPFQTIDQDGIGELIKLGIERGR
                     KTRKNLKVGICGEHGGDATSVEFCHRVGMSYVSCSPFRVPIARLAAAQAVIRGKK"
     gene            62010..>63498
                     /locus_tag="PK634_01385"
     CDS             62010..>63498
                     /locus_tag="PK634_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOW96417.1"
                     /translation="MVHKTSKQYVFAFGPGAKNTEGDASMRAVLGLRGANLAELSRLG
                     VAVPPGFTIATEVCAYFTRHGGQLPSGLLDQVDAAVRKLESLTGMKFGEARNPLTVAV
                     RCGAGVVLPGLMETVLNLGLNDQTVPAFCEQTGNARAGWDGYRLFMERFGAVVIDAEA
                     GLSQSDFDAERSKLKDKYGLAGDADLSAGHLRELCDIYKRLFFQKTRRPFPQDPREQL
                     RMTIAAGLRSWTSGRAEHYRQAHKVAGLLGAAVNVIAMVYGSLDDESGSGIVSSRDGK
                     TGSSRPVGVFRVGAQGIGLSAAASGLKDIHDMPKEKPAAWKKTYEQLAELLHKLEGHY
                     RYPQEVEFAVERGKLWILQAQNAQRSGRAAVRWALEMASGQDAVSGKPLPRVLKAEEA
                     LLTLGAPDLDTFLFPLFDAAAERQAILLARGQPLAPGAASGRIVFSLQKAGDLLRKDP
                     AARLVLVCRELGEADRANLRRVQGVLAVGAGLDSTLAAAARAQGRG"
BASE COUNT        10184 a        21435 c        21667 g        10212 t
ORIGIN      
        1 tggtgggcgt aacaggactt gaacctgtga ccccttccat gtcaaggaag tgctctagcc
       61 aactgagcta tacgccctta agaaccggtt ttttcttacc cccgaaccac atcgaaatca
      121 attcctttct cgcggcgcgg ccggcggggg cgcggagggc ttcgatgatg tggtggtcgg
      181 cgcgggaaaa ggggtaacgg ccaaactgcc ccggccggac ccacgcgaag tcggcgcagt
      241 ggatcgcgcg cggccggccg gcgcgaatgc gcgcccagtg cgcgtgcaac tcgatggtga
      301 agtggctgta ggcgtggtgc actatggtca ggagcggccc gacctcgagg cgtacaccca
      361 tttcctcgct caactcgcgc gcgatgcact ggtccatcgt ttcgcccggg tccagcccgc
      421 cgcccggaaa ttcccatagg ccaccgagca tggacgaatc cttgcgctgc gcgaccagga
      481 tgcgcccgtc gcgccggagc gtcacggccg ccccgacgac tttgtggggg acgggggcgc
      541 ggcgcttttt caccgggtac gcctcgggtt tgccgccggc gcgcgcgcgg catacgttgc
      601 cgagcgggca ggcggcgcag tccggatcgc gcggcgtgca gcataacgcg cccagttcca
      661 tctgggcctc gttgctcaag cccgcgcggc ccgggatttt cagggcttcg gcccacgccg
      721 cgagtttctg cgtcgccgcc cgggtatgaa tccggtcctc gagggccatg acccgggcga
      781 gcacgcggat tacgttgccg tccagcacgg gggcttcgtg cccgaaggcc aggctgccga
      841 tcgcggcggc cgtgtactcg ccgatcccgg gcagggcgcg gagggcggcg gggtccgatg
      901 gaaaccgccc gccgtggcgg cgcgcgatga tccgggccgc ctggtgaagg ttccgggcgc
      961 gggcgtaata gccgagcccc tcccacgcga gcaggacttc gcgttgcggg gcggcggcca
     1021 gggagcggag cgtcgggaag cgccggacga aggcgcggaa gtagggcagg gcctggtcgg
     1081 cgcgggtctg ctgcagcatg atctcggaaa tccagacccg gtagggcgtg cggcgggtcc
     1141 gccaggggaa aggccggcgg cgcttttcga accaggccgg caagcgaacg cgaaaagccc
     1201 ttttcagggc gggggacagg ctgggtccgg ggggcggcgc ggatttcatt cggggctcaa
     1261 ctccaccggg cggcgctcca gggctcggcg ctggtacacc cgcgacgagc gcgggaccag
     1321 ttcctcggcc tgcatctccc gcggcggcgt gacgagcatc aggacgacgt ccgccgtcca
     1381 ggcgaagccg cggcgggcta cgacatcgcc gatgccgacg acggtatagg tgaaggtgac
     1441 ctcgtacttg ccggccgccg tgcgccgcgc ctcccagcgc gggaacagga cgtcctgttc
     1501 ggccagttcc atctgccgct tcacccagtc ctcgttggtt ccctcgccgt cggatgcccg
     1561 ggcctcgcgg gccaggcgca gggcccgggt ttcctcctcg gcgccctcca ggacgcggtc
     1621 ctgcgggccc ggctcctgca gcacggcttc atccaccggc ccgaacggga tggccgggtc
     1681 gtccccgcag ccggcggccg ccagcagggc gcagccgagc catattaacc cggcgcggct
     1741 ttcgcggtat cttttcgtat tcacaagctg cctgatacaa catgaaagcg cgccgcgggg
     1801 caataatttc ctttgacgcc gcgggggcga agttttagtc tttccaaacg agaacgtgtg
     1861 tctgaaacgg cggatccgtc cgccgagatg gaggaacggc catggcggaa caagttcctg
     1921 acagcaagcc cgatgaagcg tccgtcccgc cccgcatcaa gctcaacggc aacggaaaga
     1981 ccgaggcgcc gccccgcatc agcctgcagc cgaagcagcc cggaaagtcc gagacctcgc
     2041 gcatcgaact gggggaggcc cggcccgcgg atccgatgga actggccaag aagatgacgg
     2101 cccgcatcga cgccccgaag ccgcccgtcc gggtcatcag cgaagtggaa cgtctcgcga
     2161 cggccaagac ggccacccag caattcaaga gcatgaccca gcaggtgaag agcgccacgg
     2221 ccccgatctc caaggccgcc attcccgacc tcgacgccgc gaagaaacag acggcccgca
     2281 tcgacctgca ggaagtgctc ggcagcgggg acgaggacat tttcaagcgc cgtacggcta
     2341 tcctggatcc cagtaagatc caggcggccg aagcggccgc ccagcccaag accatccgca
     2401 tcaagcggcc cgagggcggc acgggcccca tcaagccggt gtccgagccc atgaagccgg
     2461 ccgaaccggt ggcggcggaa gcgcgcaaga gcgagacggc ccgcatcgaa ctgccggccg
     2521 acgtggccga ggaagccgcc gccgaggccc cgacccgccg gaagacgatc cgcatcaagc
     2581 gcccggaggg cggcggggcc cccatggcga tccgcccgat gtccgtcagc cagccggcga
     2641 cggcggtgac ccgggccgag cccggcgagc tgccggtgat cgcgccggcg gaagaggggc
     2701 cgggcgccgt ctattccatc ctcgccatcg cggccctgct ggtggcctgc gtgctgatct
     2761 acgtgctggc cgcgcaggtg ggcacggtgg aaagcctgac gcccccgcat atgccgttcc
     2821 ccggcggcgt gtgaggccgc gcggaaggtc ggccatgaaa ctggaagacc tcgcctacgc
     2881 cattgcccag aaggtgttct gggagctcga acacacgcat catttcgtgg tgcccgaagc
     2941 cgtccgcaag gaaattctgg ccgcggtgcg ggcccaactc cactcgctga tcaagtagcc
     3001 ccaaggagca ggtaacatga cggctgccgg catcgttcac gtcaacgcgc agaactggaa
     3061 gatcgaggtg gcggactccc cggtgccggt gctggtggac ttctgggccg agtggtgcgg
     3121 gccctgccgc gtcatcgcgc ccgtcctgga cgaattgagc ggggaactgg ccagccgcct
     3181 gaagatcgcc aaggtcaacg tggacgaaag ccccgagctc gcgggcgact acggcatccg
     3241 ctccatcccg acgctgctcc tggtcaaggg cggggtcgtg cagcagcaga tggtcggcgc
     3301 catgagcaag gcggcgctga agacgcggct ggagtccgaa ctggactgat cacgcgtcct
     3361 tcgcgcacag ggcggccggg ggcagcaggg cctgcaattc ccccttgagc tgccggttga
     3421 cttccgccac ggcgcacgcg gctttcttga accgcacccg cagcgccccg gcgccggcga
     3481 catgacccag tatatgggac aggtccggct cgtggcccac cacggcgacc gcctcgggaa
     3541 gggccggcca ctccgccagc gcccggacca ggtcctccgc gccgcagccc ggattcaagc
     3601 gctcggaggc gacctgcgcg gggtttccca ggcaatccgc cagcagcgcg cccgtctcga
     3661 tcgcgcgccg ggcgcgggag gtcaagatga gctgcacatc cggatgcagg acggccaggg
     3721 tcttgaacgc gcggcgcgcc ttcttccgcc cctcgtcggt cagcggccgg tcgaggtctg
     3781 atcccccgaa atcctccggc tccgcggccg ccgcgtggcg aatcagcagg agtttcatgg
     3841 attaccgagc cgtttctttt ggctggactc ccgccgcccg gtcattgtat gagtccgccc
     3901 gttgcccgat ggtgtaatgg tagcacaact gactctggat cagtttgtct aggttcaaat
     3961 cctagtcggg cagccacctg cgccgaccgc ctccctagct caaccggatc ggcatcagca
     4021 cgtacaggaa cggcaggtcg cacttgagca cgccggggct catgtcgtcc gtgagctcca
     4081 agtagatctc gtcgcagacc aggttcttga gcggatccag cacgaagtcc gggttgaacg
     4141 ccacggaaat ctgcttgccg gcgtaactga tcgccagggt ctcgcgggcc tcgcccacct
     4201 cgggcgaagt cgccgagacc tcgagcttgt tcttggagaa cgtcagccgc accgagttgg
     4261 agccctcgct cgcgatcagc gcgacgcgcc ggaccacggt catcagggcc tcgcgctcga
     4321 tcttcacccg ctcctcgctc tgcgcgggaa tgacctgccg gaaattgggg tagctcccct
     4381 ccatgagctt cgagaccacg agcaggtcgt tgaactcgaa ggcggcctgc ttgttcacgg
     4441 cgtggatctt caggctgccc tcggcgccca gcgtccggga gagctcggcc acggtcttgg
     4501 cggggacgat cagttcggcc tcggcgtcct tcgggaactc cacctcctgc tcgtagagcg
     4561 ccatccggcg cccgtcggtg gccaccacgg agagcttgtc gcccttgaag ctcaacagca
     4621 cgccgttcag cgtcaggcgg ctctcgtccg tggacgccgc gtagcccacg ttcagcagca
     4681 tgtcccggaa cacccgctgg tccagcgtgt acgttttgcc gccgtcgaat ttcggcaggg
     4741 gcgggaattc ctcctccgag atgccgatga tcttgaagaa cgccgagccg ctccggatgg
     4801 cggtctggtc cttgtcgtcc gcctcgatct cgatgtcgcc ggccggcagt tccttgaaga
     4861 tgcccgcgca gcgccgggcc ggcagcgtcg tggcgccggc cttggacacc tcggccgcca
     4921 cgcctgcccg gacgctcaag tccaggtcgg tggcggagag ccatagcttc tccttgtccg
     4981 ccttgaaaag gatgttctga aggatgggca gcgtcgtgcg cgtgctgacg acgggttgaa
     5041 ccttctgcag cccctccagc agggcgtcct tggcaatctt gagcttcatg actccacctc
     5101 gttttttctc cacagccttt tctggggata cgctgaaacc atttccatct cttcctacta
     5161 ttatgtcctg tgcatatgtt gaagatcaag aaatcatccc gcccgcaggc gccgcgcgcc
     5221 cccggcggcc tgtggacaac cctgtgcaga gcctccgcac atccgggccc cgctgtgcat
     5281 aacgcgggct tgtccacatc tcgcgtcccg atgcgcgcgg ctctccgcag ggttgttcac
     5341 agggtccgtc gcgcgcatgg gctcacccgc gggtcagccg ctgcttgagg acgctgacgg
     5401 tctgccgcag cgccgtgtcg gtcttcatcc gctcggccac ctgccggcag gcgtagatca
     5461 ccgtcgcgtg gttgcggccg aaccgctccg cgatcacggg cagcgactgg ttcgtcagcg
     5521 tgcggcacag gtacatggcc acctgccgcg gcatggcgat ggcctgcgag cgctggcggc
     5581 ccgtcatgtc gccgagccga atgtcgtaat ggtccgcgac ggcccgctgg atcgcctccg
     5641 cgctcaaggt ctccttctgc gcctggtcga tcttgtcgcg cagcaggtac tcgaccgtct
     5701 cctgcgtcac gggcttctgc gtcagggagg cgtaggagac cacccgcgtc agggcgccct
     5761 ccaggctgcg gatgttcgac cggatgatct ccgcgatgaa atggacgatc gagtccggga
     5821 gctgcaggct gcaggtctgc tgcttctggc gcaggatcgc gatccgggtc tcgatgtccg
     5881 gcggctcgag ctcggcgacc atgccccact cgaaccgcgt caccaggcgg tgctcgatcg
     5941 ccgggatctc gctggcgggc cggtcgcagg tcagcacgat ctgcttgcgg ctctcgtaca
     6001 gggcgttgaa cgtgtggaag aactcctcct gcaggccctc cttgccggcc aggaagtgca
     6061 cgtcgtcgat cagcagcatg tcgtaactgc ggaaccgctt ccggaaatcc accagcgcct
     6121 tcttctgcag cgcgtcgatg tacgcgttcg tgaaggcctc gcaggaggtg tagaagacgg
     6181 agagcttgcc gtgcggcagc acgtggttgc cgatcgcctg catcaggtgc gtcttgccga
     6241 ggcccgtgcc gccgtagagg aacagcgggt tgtaggcgtg ggccggcgag tcggcgacgg
     6301 cgagcgccgc cgcgtggccg aggttgttcg acgagcccac gatgaagttc tcgaacgtgt
     6361 accccgcgtt caggttcggc gagccggcgg ccggcttgcg gcgcgggggc ctctccacgc
     6421 ccggcgccgt cgcccgcgcg ggcgcgatgg tgcgggagga gtccaccgtc agctggatct
     6481 gcaccggcgc gccgcagacc gcgctgacgg ccttgcggat caggggaagg tagttctctt
     6541 ccagccaggt ctggtagaag tcgttcgcga cgctcaagac catcgtgtcc gaagaaaaac
     6601 tctccggctg gatcaccgcg atccaccggt cgaagatgtc cttcggcagc gtctccgaaa
     6661 gatgctcgca ggtcttcgac cacacgagcg atgcctgcat ttccacagtt aacccctccg
     6721 tttcagagtg ccttcaacat ccgcaagaga gaaggctaaa ccattcacgg atccgaagcc
     6781 tctgaacacc ctgaacgtcc gcgccgccgc gaactcctgt ggaaagcaca agtccccggc
     6841 ccccgccggt caaaaagccg ctatcttcca cttgacttct ctgcgctaca tgccagtgat
     6901 aattagctta gctttttaaa acagttacag cagatgccga agacttgttc cgcagcttat
     6961 ccacaagtta ttaacagtat tcagtgcttg tccgtgtgcc tgtacggccg caaccttata
     7021 cggagtttgt gcggcgacaa gacaaatgat gaaaaaaaac gcaaagaaat gggaggccac
     7081 aaggaatggt ggtccgtggt tttttgccca cgtaatattg gattttttcg acacggttta
     7141 cgtgcgcggc tgaaacgctt ttttaatgga atcgaaataa tgcagtcagc gatgcgcgcc
     7201 ggaaacagcc gaaaaaccgt caaacggcgc gattatccga ggcaacccga atgaaaaagt
     7261 taacagggcc gcttttaatc gccggttcgc ccgaaaaagt gcccgatttg ctctatgcca
     7321 cgggcttttc cgccgtggat cccgtggtct atctgcagga ccgcggccgc cgctggctcg
     7381 tcgtgcccct gctggaggcc ggccgcgccc gccggcaggc caaaaagggc gtcgaaatcc
     7441 tcattccggc cgaactggtc cgccgccgct ccgcccagcg ccgccgggac cagtggatcc
     7501 tggccctgct gcgccgcgcg cgggcgcggc gggtgacggt gcccggctac tttcccgtgg
     7561 acctggtccg gcggctcgag cggcgcggca tccgcgtgcg cgtggcgccc ggctcggtgt
     7621 tcccggagcg cgaggtgaag gacgcggcgg aactccgccg cctgcgtctc gcgcagcgcg
     7681 ccgcgatccg cgcgatgcgg cgcgcggtcg gcctcctgcg ccgggcccgc gcgcgcgccg
     7741 acggactcct ggaactggaa ggccgcgtcc tgacttccga gcgagtgcgc cacgagatcg
     7801 acacgacgct tctcgagcac gattgcatcg cgacggagac catcgcggcg ggcggcacgc
     7861 agggcgccga tccccacgag cgcgggcgcg gcccgctccg ggccggcgaa ccgatcgtga
     7921 tcgatatctt tccgcgccgc cgcgcggacg ggtactgggg cgacctgacg cgcaccgtcg
     7981 tgcgcgggca ggcctcgccg gcggtgcgga agatgcacga ggccgtccgg cgcgcgcagc
     8041 gggccgcctt gcgcgggatc cgggccggcg cggcggtcgc gccgatccac cgcgccgcgc
     8101 agcgcgcgct cgaggcggcg ggcttcgcca ccgcgggacg cggcgccgag gcggagggtt
     8161 tcatccataa cacgggccac ggcgtgggcc tcgaaatcca cgagggcccg atcgtggggg
     8221 acgcgcccgg ccggctgcgc gcgggccacg tgatcaccgt cgagcccggc ctctactacc
     8281 ggcgtcacgg cggcgtgcgc atcgaggaca ccattgtcgt gaccgcgaag ggcagccgcc
     8341 cgctggcgcg gtgcccggat tatttcgagt tgtgacgatg gacgagtggc tttcattcct
     8401 ggcgcgcggc gggccgttcg tcctggtccc ggcggcgctc gcggccgggt tctggctgtt
     8461 cgagtggacg aagatctacc ggccctcgcg cctcctcgac acgtcgccgg ccgccgtgca
     8521 gctggactac gaggacgtcg acttcgtcgc cgaggacggg cggccgctgg ccggatggtg
     8581 gatcccgcac ccgcgggcgc gcgggacgat gctgcattgc cccggccagt cgggcaacat
     8641 gggcgaccgg ctgcacctcg ccgtggacta ccaccagctc gggatgaacg tgttcctgtt
     8701 cgactaccgg gggtacggcc ggagccgggg catcccgacg gagaagggca cctaccgcga
     8761 cgcgcgggcg gccttcgagg tgatccgcgc ccgctacggg gacgccgagg cgccgcccgt
     8821 ggtggtgcac ggcgtgtcgc tcggcggcgc ggtggccgtg cagctcgcgc tcgacaagcc
     8881 ggtgcggggg ctcatcctcg aaggcgcgtt cacgtccatc cgcgacatgg gcgcgcgcct
     8941 gtacccgtgg ctgccgatgc gctggctttc tcggttccgg tacgactcgc tcgccaagat
     9001 gccgcgcctg aaaacgccgc tgctgctggc ccacagccgg gaggacgagc gcacgccgtt
     9061 cgagttcggg cgccggctgt acgaggccgc ggcggagccg aaacggatgg tggaactgac
     9121 cggccggcac cgcgacgccg gctggaaccg gaccccggag tacggccggg cgctgcagga
     9181 tttcctcgat cgcgtcctcg ggccccttcc gcgggacaat aattgaacgc gcgccccgta
     9241 ttatggtcag acacggcaca acagtgcgga aaggagcgga tatgaagaac ggattaaggt
     9301 attggggcgc ggccctgctg gcgggcgccg cgatcgcggc gcaggcgcag ggcccgatgg
     9361 gcggcccggg catgccgggt tgcgccatgc agggcggggg cggttgtccc atggcgggcg
     9421 gcgggcggga acaggccctc aaggccgcgg gcgccacgga cgaacaggtg gcccgggtcc
     9481 gcgagaagat gtacgagacg cgcaagcaga tgatcgcgct gcgcgccgaa caggaactgg
     9541 cccggatgga ggtccagcac ctgttgagcc aggacaaggt cgaccgggag gccgtcatga
     9601 aggcggttga caagaccggc gccgtggaga ccagcatcca caagctccgc gtccagcaac
     9661 agctcgacct gcgcgacatc gtcggcgcgg acgtcctgaa gaaggcgcgc gagtccgcct
     9721 tcgagcaccg gcgcgacaag caggacggac gtcgcgagga ccggcgcggt gaccgctggg
     9781 gcggccgggg tgaaggaaaa cgtggcgagg gcgaggggaa ccgcggccga ttccagccgc
     9841 ctccgccgtt ggagcccgaa gaaatacccg ccgaggaacc gctggtggaa taacaggcga
     9901 ggggtgggtg caggggcgcc gctagtcggc gccctttttt ttaatgctcc ccgctgggcg
     9961 tgggatgcga ccccgggatc ggccgtccga tcatcaggcg gaagccgaac cccgggtggc
    10021 tccggtgggg ggcgggtgtc cggaagaaga gaaactggcg ctcgccctca tgcgtgccga
    10081 accagcacgc gccgcgtatg atccggcggt tcatgtcggg atcgaagatg tcctcggtcc
    10141 actcccacag accgttttcc agcgcgcaca gattccaggc gttcggcgag tcatcgacgc
    10201 ggtgttccag cggcaggttt tccaccacct cgaggcagcc aagccccggg cagcggtcac
    10261 cccacgccgg gccgtctgac cgttcgccgc gcgcgcagct ggtccattcc cactggcgga
    10321 gaaggcgaaa gacatgtccc ggcgctacgg cttccgcgtc gaactgggcg ttcatccagg
    10381 aacagaacgc ccgcgcgcgg tcccagggcg ccccggtcag cgggcggccg gtgttcggga
    10441 tatgcgcgtc gaagccatgg aattggccct cggtgacggg catcggctgc acccatccgt
    10501 ccagggccgc cacgcggacc agtcgtaccg tgacgcccgg ggcgatcgac agcgtcagct
    10561 cgctcttgtc ctggccggca ggatccatac cggtgagtgt agcaggaaac gtgccggtcg
    10621 gcagccccgg cggaagggat tgacgaccgg cggggcaatc cgtatggtat gcccgttttt
    10681 ctgtggcccc catcgtctag aggcctagga cgccaggttc tcatcctggt aacatgggtt
    10741 cgaatcccgt tgggggcgcc acgatctctc ctccccgcgc ggccggcgcg ggcgtccgat
    10801 gcggatcagg cccgggcggg cgcgaggtcg tggggcgaca cggcgtgtaa ctccatgccg
    10861 ggatgctcct tgacgaagta ttccagcgcc caggtgttcg ggaagaggat gaccgtctgg
    10921 ccgcggacgt cccgggccag cttgacgccg gaaccgagga acccgccttc caaggcgccc
    10981 gtgtccgcgc cgggcgcgaa ccagcgtatc tgggcgaacg gcgcgggctc catcaccggc
    11041 tccgcgccgt actcgttctg cagccggtac atcaccacct ggaactgcag ctcgccgacg
    11101 gcgcccagca gggccgtgtt ctgcggcacg tccgacgagg tgaaggactg gatcactccc
    11161 tcctgcagca gttgatccag gccggcgcgg aactgcttgt acttggccgt cccggaattg
    11221 cgcaggtagg caaaggcctc gggcgggaac cgggggatct cgtcgtagcg gatcgccggg
    11281 tccgtcgtca gcgtgtcgcc gatccggaaa gcccggtgcg aaacgaggcc gacgatgtcg
    11341 cccggatagg cgatttcgag cgactcgcgg tcctggccga aaagcttctg gggataggac
    11401 agccggatcg gcttgccggt ctccgggtcc gcgacgacca tatccttgac gaagaggccc
    11461 gagcatacgc ggacgaaggt cagccggtcc cggtgcttcg ggttcatatt cgcctgcact
    11521 ttgaacacga atccggaaaa ctccgggtgt gtgggctcga tcttgccgtg ccccacctcg
    11581 cgcggctggg gcggcgcgcc gtgctggacg aagtagtgca gcagcagctc gacgccgaaa
    11641 ttattcatgg cgctgccgaa gaagaccggc gtgatcagcc cggccatgac atgctcctcg
    11701 tcccacgcct cgccggcgcc ggcgagcatc tcgagttcct ccagccatgg atcgtacacg
    11761 cgggccggga tctgcccgcg gatgctctcg tcccggatgc cgctgacccg ggtcggggcg
    11821 cggtgctgcc cgtgggccgt gcgctcgaac aggtgcaggt tatgctccag ccggtcgtag
    11881 accccgcaga actcgtcgcc gctgcccagc ggccacgtga cggggaacgc cccgatgccc
    11941 agcaccttct ccagttcgtc gagcagggcc agcgggttgc gggacgggcg gtccatcttg
    12001 ttcacgtacg tgaagatggg gatcccgcgc atccggcaga tttcgaaaag cttgcgcgtg
    12061 cgctcctcga tgcccttggc cgcgtcgatc accatgatca cgctgtccac ggcggtcagc
    12121 acgcggtagg tgtcctcgct gaagtccttg tggccgggcg tgtcgagcag gttgatcacg
    12181 cagcccccgt actcgaactg gagcagcgtg gacgagatgg agatgccgcg ctccttttcc
    12241 agctccatcc agtccgacgt cgtgctgcgc tggttccggc gcgcgcgcac cgagccggcg
    12301 agatggatcg cgccgccgta gagcagcagc ttctccgtca gcgtcgtctt gcccgcgtcc
    12361 gggtgcgaga tgatcgcgaa ggtgcggcgg cgggcgatct cggttgttag ggccaggtcg
    12421 gtcacgggaa actccgacgg gtcagcgcga gcgcttacag gcagaagccg atggttccgg
    12481 cgtggtcatg gggcgcgcag gatagaaaag gggcgacgcg gtgtcaaacc aggaaaggac
    12541 ggcggcgtcc gccaccagcg ggaaaagggt cacagtcgaa gaaatccggc ccatccggct
    12601 tcatcactat tcatgggcgg catggtctat gcctcccggg cctacggcga cggccgtatc
    12661 ctgttcggcc ccggctgcac gatatcgctg gatatggaca cggggttcat ggacgccgga
    12721 tcgcgcgtgg gcttcagcgt gatcgggacc ggcgactcgc ggacctacgg cgacatcagc
    12781 gtgtacttca cgggcggagg cgccaacgat atcgtgagcc agggcagctg gtactgggtc
    12841 accacctgcg acacgggcat ccctttcgac tccaaggggc tccacgtgga gatcgatatc
    12901 caggagacca acaacttcac ctgccggctc acgccggcgg ccagaacgcc ggcgccggca
    12961 gcacgaacga cgccttcttc aataatatcc acgtgctgcc cccgccgcgc atcaccgact
    13021 tcgcgctgac ggacgggacc aatgccgcgg tccgtttcct gccccgcccg gagttgaaaa
    13081 acgccgtgca ggcctgcaac gatctctcgg ccggcaactg ggtgccggtg gccacgaacc
    13141 tgaccggcac ggggctcgac gcgctacagg ttaatgatgc cgtcgccgcg gatgtcgcaa
    13201 gccgttatta ccgcatccgg gccgcgccct gaaccgtcgc gcgtcgcact tcaatccgct
    13261 ttcttccaag gagcttcgcc tccggctcga acccgcgagc cgatggcaaa gccgtttcca
    13321 ggtggccgcg cggcgcctca acgtcgtgtc ccttgccgac ccgccttcgc gggaaacgcc
    13381 ggtggagcag caatgccgtg ctgttcataa tgccgcgcgg cagtgacctc ggtcgctttg
    13441 gcggcgtgcc ctccctgtcc agagagaacc cgttgcgagc gaaataattc aaaaaaacgt
    13501 ggaatgcccc gggaccggtt cctttctctg aatgggtgcg attacccatt ggagggcccc
    13561 atgacatggc cgtagcagca caaggtcgtt gacaaaggct ggggtatcag gaggtacggg
    13621 ccggcaacac gtacggcacg atggcgggac catagcccgt gttatggaca gcaaacacgg
    13681 acagtaaagg gtgtgatggg cagggcgatg ccttcggcgg agccgcggtc cggcgggaag
    13741 cacaccctgc cctttctgcc tcggccgacc tgcacctggt aggacgggca ggaaggacgt
    13801 ccattcctcg aaagacggcg ccggcatcct gcgcttgggg atcgcggacg cggcggggat
    13861 taaggacgcg gcggggaagg gcggacacgg ggagggaaag ccttgccgta atgtgtctgt
    13921 agtggccggc gcgccctgaa tcgcggtgtg caacgtcagg atgacagtgc ccgcgggccg
    13981 gcaggggaag cggcggatgc gccccctacg gccccacggc cgtccggtag aatccggggg
    14041 ggaccgcctc gaaggtcagc gtactgtcga ggacggagtc cgtggccatg ttgagccagg
    14101 tcgggttcag cagggttgcg gagccctgca ggatgattcg ctggcccgcc gcgccctgcg
    14161 cggtcaaccg ggtggctccg tgcggcccct cttgtatcgc cgtcaggtgc ggctcgggtt
    14221 cggtcatgcg gcccagtcgc aatccgtttc cgaagctggt gacccatatc tcgttggtgt
    14281 catggggatt gaaaaacacg cgggagggga atcggaaggg ataattggtc aacgcggtga
    14341 aggacggcgt ggccgcgcgg agattggtgc tgacccataa gccctggttc tccgtggtga
    14401 cgtagacctg gtcggagtcg gcgggatgga ttgtgcagga gccggcctgg tcgatgttcg
    14461 tcgtgatgcg cgtccaggcg aggccgcggt tcgtcgtgcg atacagtccg cccaggccct
    14521 ccgagttgcc ccactcgccc cagaccccgg cgtaccacgt ccgctgggcg ggatcgtggg
    14581 gatccggcac gacgtccttg gtgtagtact gcatgccggc cgcggagcga tcgagccaac
    14641 tggagccggc gttcgtgctg acgaatacgc cggagctggc cgtgaagtcg ccgctgatcc
    14701 gcgcggagta ggaacagacg agcgtcccgt cgttcaggat atggacgacg taggggtggc
    14761 cctcggtccg cgtcgggttg gccagcttgg tccaggtcga gcccgtgccg gcctgcaggt
    14821 tggtggtcag gaagatgccg cccagcgtgc tgtggacgac cgaggcgatc atccgccggt
    14881 cgttgttcgg gtcgagggcg aggccgacga cgggatgggc aaagtcgtga agcattttcc
    14941 agccccgccc gttgttatcg gagtaaatga tggcgccgcc cccgctgtcg atccgcgagt
    15001 cctggcagta ggcgtcccag gcatacaggt cgtggacgga agacgcggcg gcatacaaga
    15061 cgccgctcga tggtcgccgg acactctggt acgtgctgtt gtacgtcagg cccgtcggga
    15121 aataccacgc cgcgccgccg ttggtgctgg ccacgccgcg catgtcggtg aaacaggcgt
    15181 agaggtcgtt cggcgcggcc caggtgagcc accagcagga cgtgtcctcc aggccgatgc
    15241 cgaggtagct cttgcgcttg tcggacgggc tgttggtcgc gttctggtcg gagggctgcg
    15301 cgtaggcctg gctccagttc accccgccgt tggtcgtgac gtgcacgaac ccgagatcgc
    15361 tgatcgccgc gcggttgggg tccgtcgggc acacgttgaa cccgagggcg tactcgccgt
    15421 acgaccactt catccaattc cacgggcccg ggtcgtcgcc ctgccagccg gtggcgatgt
    15481 tctggttgcc ggatgcgaag aacaccttct gccatgaacc gccgctgttc gtggtcttgt
    15541 acacgatggg cgcgccgtcg tccgagccgc cggcgacgta cgcgatgttg atattggtcc
    15601 ggcacatgga gacgaagaac gggtagtcgc ccgcggcgat gccggtcacc acggaggtcc
    15661 acgccgcgcc cggcccgacc gtcagcgcgt agaggcccag gtagccgtag tactcggaac
    15721 ccgtgatgcc gggccagatg ctgccggaat ccatggtcac gcagaacaag cgcaccgttc
    15781 catcctggcg ggcgcccgcg aacgacagga tgccctggcc ggcggggatc cctgcggtcg
    15841 ccggcttcgc gaaggtgatg ccgccgttgg tggagacgat catccccgcg cgcgtgccga
    15901 cgtagatatt gctgccgtcg aagaacgcgc ccgcgacgtg gttatcgttg gtcctgaaaa
    15961 ccagcgagta attcgtgccg ccctgcgagg agaaatagac gtccgtgtag tcggccgtga
    16021 ggacgcggtt ggtgcgctgg gggtccgcga agacgacgta ggattcccgc gtccagggat
    16081 ccagcagaat ttcatgccag gtgcgcccgc cgtcgtcgct tttcatgggc gagtaatagc
    16141 tcaagccgta gagcacgttc ggatcgctgg tgaactggac ccaggtcatg cggttgtggc
    16201 cgctggtctg gcggaagtcc agcgtcaccc acgactgccc gaagtccgcg gaccggaagg
    16261 ccagccccat gtcgcaactc acgaagaggt cgttcggatc gtgcgggctg atgctcggcc
    16321 cgaacagcgc gccgcccccg cccggcccgc gcgagatcca tcgggccggc gtcgcggccc
    16381 acgccgcgga tgcgaggagg agcgtcgcca gccctatcgc cgttcggaac ggtttcatgc
    16441 ttctccattt gcagcaactc gcatgccaaa gcatgcggga ttcgtatcga ataccgggat
    16501 caggacgagg gcctggagag cgccttgtcc agccggcggg cggccttcaa ccaggcgggc
    16561 aaatcggggc cgcggcgcag ggcgagctcg ttcagccggt tcagcgggag ccacagccag
    16621 cggtagaatc gcgacgacag gggccggcgc cagttgcgct gccgcgccag gccgacgatt
    16681 tcgcggtgca gggattcgcg gctgctccat accttcccga tgaagctgtt ccggcggcga
    16741 cggtacgcgg cgaggtcgaa gacggcggca taccggtcca gcacgccgcc gatgtccagc
    16801 gtgaggagcg tccgcaggca cttccagcag gtggagcagt tgccggcgga ggccaggccc
    16861 gcgcagacgt tcagcgaggt ccacgtgtcg ggaatcccgg cgaccttcag cgtcttctcg
    16921 acccgcgtgt attcgccgcc cgccgaaatc gtgtcgagcc ggtccgtcga gagcagcggc
    16981 agcgtcacgg cgtcggcgtg cgccatggtg taggtggggt ccaggcgcag gtcggcgtag
    17041 gaaaagcccg cggcgtacag gaagcgcccg atgccgccct ggagcgcgtg ccccaccgag
    17101 acgttgcgct gggtgtgggt caggaggttg cccaggcccg gccggtagaa ctccgcgacg
    17161 ttggagttca cccggaccag cggcaggccc atgcgttcgg cgtggccccg gaggcgctcg
    17221 catcgctccc acatgacccg gtcgcccgcc tctccgaacg cgcccgtccg gctgaagagc
    17281 aggtgcgtga tccggaatcc ctccgggggc cgggcgtaat ggtggtcggc cagcacgcag
    17341 aaggaatcga tccccccgct gaacgcggtc gccacgccgg gcgcgcgcgg actgtcggag
    17401 tgtgtcgcct cggcgcggat cgccaccggg cgcaggaaag gcatgaggcc tcggagcacg
    17461 gcctggtaac agccgttcag ggcatagagg agccgggcgg acaccgcccc gtcgagccgg
    17521 atgtcctcgc cggcgtccat ggcggggatg agcagggcca ccagcgcggc gtcgctcaac
    17581 tccgacagcc agtccccgtg ctccgcgggc acgcggtacc acagaacccc cgcgcccttc
    17641 gaccactcca cggcggcgcg gcactcggcc tcgccgtcct tccggagtat ttcaggacgc
    17701 ccgattatca tgccgaagag gctgccagag ccgggatggg gagtcgagaa cgaaggccgc
    17761 tacgccttcc ttgcgcgctg ttcgcgcgtg cggcatcatg cccgccggga agaaacaggc
    17821 gatggaaacg ctgcggctcc acatgacctt cgggctgatc cagcagatca ggctggtcca
    17881 cgccctcgcg gaatccagcc atcccgtctc catcccggcg tccgaggagc ccaagttcga
    17941 cgccctccgc gcgcggttcg gattccggtt cgcgccgccc gatgccccgg gcgtcgacgt
    18001 gacccgcttc gtgcggtgcg agcaccgggc gccggcgacc ttcatcggcc ggatcgagcg
    18061 gccggtgatt ttcccgcggg ccatggcgga ctattgccgg tcgctgtggc cggcccggcg
    18121 cgcgcaccgg ttttcgttcc agggcctcgt cttcggcgcg cgcagaacgc tgctgcagaa
    18181 gtggatcaag gccaacctcg gcggaaggac gcctcgcctc ccggacgcgg agggcgcggt
    18241 ggcccgctgg cgcaacaagc tgttccgcgc cgccggcctc gatcccacgc gcaagcagcg
    18301 catcggcgac ctgctgctct gggaatccag ccggggccgg cgcttcccgg tcaaggcgtg
    18361 ggacgaggaa tacgtcggcg cgctcgcggc ttcggaattc gtcctgtgcc cggatggcga
    18421 gcacgtctgg acctaccggc tcttcgaatc cgcgctgtgt ggcgccatcc cgatcgtcga
    18481 gcgggcgtgc cccgcgtacg ccgggttccg cttccgctcc ttcgaggacc gggccggccg
    18541 cctgacctgg tcggccgaag acgcggaaca caattaccgg ctctgcgcgg aacggctgac
    18601 cattgcccag cccacgctgg acgccgagat cgcgcatatc ctggaacagg cgcgttgatc
    18661 cggcgccggt cggcgcgagc ccggcgagct actgcttttt caggcggtag aaataccggt
    18721 cggaccccgg cgagagcgcg caggtattcg tgtggccggc ccggttggtg aacgaggcga
    18781 cgtgcgacca gctggccgtc ggcgccaggc tcccggcgcc gagcacctcg tagacggcgc
    18841 cggtgcagag gctgctccac tccacgcgga cctgggcgcc cgcgcactgg atctgaccgc
    18901 cctccgggcc gccgggccag ccggcgagcc gggccatcat ccaccagaac gccgcgccct
    18961 tgcggttgca gttgaggtag accgagtgcg cgcagccgca gtccccgcag gagctgtccc
    19021 cgacgttgag ccagtgcgcg ggatgggccg ccgcccactc gatggcccag ttcccgtcgc
    19081 cccagggatt ctcgtccccg ttcgtgtcgt actcgcagcc gtcggtggcg aaccgggaca
    19141 ggaattccgc gccgtccggg ttgtagctct cgatgtcggc gaaatcgaag aggatcttgt
    19201 tgttcgctcg gcagaaggcc cggatctggt tgttgcggac gtgcaggttg ccgttggtcc
    19261 ccgtgccgtc gaggtggccg gtcatgtaga tgaagaggat gttggtgtac tgctgctcga
    19321 gggcgtgcat ggcctggagg taggcgttga tgccgtcctc gtcgttgtcc gaaacgccgc
    19381 cgcaccacga ccacatcacc acgttgcggt cgttgcccgg ctcggcgagc atggcggtgg
    19441 tcgcgtcccg ccaggagagg tcgccggagt ggccgaggtc gcccgcgccg ccggcgttgc
    19501 cccactcgtc gttcagaaaa tagccggggt tcagccccca ggacgcggag gtgtagtaat
    19561 agcgcgatcc ctccacgccc cggaaattct ccatcccgga gaccagctgg ctgccgtgcg
    19621 aggtgtggcc gtagccgatc cggaactgcg cccgcgcctg gtccagccag tagtccggga
    19681 tctgccacag atcggcgcag gcgtgatcga tgaccagcgg cgcgtcccgt cccgcggccg
    19741 cggcggcgag cgccacccat gcgaggaatg cgagaccccg gttcatgccc cccaatgtac
    19801 cctcccgacc cgggcgccgt aaacccgggc ggctcatggc ttcgccagct cgacgcgcag
    19861 cagcgggctc ggccgctgct cgctggtgac gtacagcgcc gagccgtccg ccgcgtagca
    19921 gagcgactcg gcctggcgga gccgcggcag caggatcggc tccggctcgc gctggatggc
    19981 tgcggcccag tcctccccgg gggcgcgccg gaacaggtac gcgtgcttgt acgtgagcgc
    20041 cgcggcggcc gaaccgtcgg gggcgaagtc catcgcggtc acctgcgagc ggaaccggcc
    20101 gttcttgcgg tcctccgcca agtcctcggc ggtgggctgc ggaatccgcg gcgcgtcggc
    20161 cacgcgccgg gcggtcagga cggtctcgac gccggtcggc gccaggggca gcgcgtagag
    20221 caggggggga tggtcgcgct tggtgagcag gtagacggtc tccccggcgg ggtccaccgc
    20281 gaccgcctcg cagtcgcgcg ggccgccttc gtacgtgaag cgcagcgtcc gccacaccgg
    20341 gaccgcgccc cgcacggcgg ccaggccggg cggcacggcc ggctccttga cgatgtgcag
    20401 gctgctgtcc gggtgcacgg ccttgttgtc gccggtgtcg gcgatcagga ggcacgcggc
    20461 cccgcccatc gtgaaggagt cgatgtcctc ccagtcgcgg ttcgtcacgc ccacgacggt
    20521 gaagaccgcc cggtgcgatc cgtcggggcc gacggcgaag agttcgggcg ggttcgcgct
    20581 gtccgtgagg gcccagaaca ggccgggttg ccgcagcgag ggcgcgagcc cgctgacctc
    20641 atccagctcc cgggcttcca gcagcccctc gctcacgacc cgcggcgccg cggcggtttc
    20701 ctgggcgcag agcgcccgcg ccccgcccag ggccgcgacg agaatggccg ccatgcgcct
    20761 catgactccg tcgcgggagg ccggcggttc agggggcctt ggacttctgc gcctcggcga
    20821 gccgtcccgt gttttccgcg atgcggaaca cggcgatgat catctcgcac cagatgcgga
    20881 tcagcaggac caccaccagg aacaggatcg gcgtgaccac gagggacagg aggccgctcc
    20941 cgaacccgct gaagcggaac gccaggtaca cgcgctgcag ccccgccagg gccgcgccga
    21001 tgatcccgat cacgtacacg accttgatca gccgcgtcgt cacgaactcc gtgaacgaca
    21061 ggtcgaacag cgtttccagg aaacttttct cttccatgat gcgctccttt ggatgagccc
    21121 ttgtgcgcag gcagtatacg gcaaggcggg gaggcgggca agccggcggc ggggccgcgc
    21181 ggcggagcgg agggccgttt tattcagtga aaacgcttcg ccccggcttg attcggcggg
    21241 gcgtgtgtgt ttacatgccg cgaaagccgg cggagagaac ggaatggcta ggagagcatg
    21301 gtggatcgcg tggatcgggc tcgcggcggg cgtgttcgcc gccgagtcgc ccccgctgcg
    21361 ccccgagctg gactacatcc ccggcgacgt gcccacgccg tggctccgct accagaaccc
    21421 tgcggcgcgc ctggtcgagg tctgcggctc ttgggaccag tgggcgaagc gccacgccct
    21481 ggagctcaag ggcggcgtgt ggaccctcga cacgcgggtc ctccccgggc gctacgggca
    21541 gtacacctac aagttcgtcg tcaacggcga ctgggaaaag ggcgccgacc gaacgctgtt
    21601 cctgaacggc gccggcgaga tggaaaagca gtccgacgcg atcttcgacg cgaccgtcga
    21661 gggccggcgc gagatccgcg tccggttccg cgacgagctg ccggagaacc cggcgctgac
    21721 cgtccgcctg tcggacggca acccggtgaa ggaatggtcg ctcgtcacga cccgcgagaa
    21781 gggcagcctc cagggctatt tcatctcggg gagcaccatc accttcgtgt tcgacgagaa
    21841 ggcctatttc cgcaagctga cccgctcgga caaggtggtc gtcgccggca acttcacgtg
    21901 gtgggacggc agcggcggca acgggcgctg gctcctgaag ccgacctcgg ccggcacgcg
    21961 cgagatgacg acgcagctcg acggcctgcg cgccccgccc ggcgagtcgg acctgctctt
    22021 caagttcgtc atcaacgagc aggagtggct gccgccggcc gccggcgcgc ccaacgccgt
    22081 gacggacgcc aacggcaccg agaacctgcg cgtggacgcg accctgctgg gcgggaccaa
    22141 gctcaagatc atcacggaac ggcccgtgga cctctcgcgc acgaccgcgg tcatcctgga
    22201 gggcctgggg gagcggccgg tctggcgcct gctgacgccg ggcggcgtgt tcgacacgtt
    22261 cgtgtcggat aaacccctcg gcgccaacct cgaccgcgag cacggctgca ccacctaccg
    22321 gcttttcgcg ccccgcgcca gccaggtcca cctgaacctg tacgagggcc cgaaattcga
    22381 ggtctacaag cccgcgttca agcggctgga tccggcggaa cgctacccga tgtggaagga
    22441 cccggaggac ggcgtctggg agatctcgct gcagggcctc gacacgggcc gctactactc
    22501 cttcaacatc gacgggccga tgggcaacgg cgagggcttc aacgtgtggg ccaacgtcgg
    22561 cgacccgtac gcgcgggccg tcgcgcacgc ggagaacaac gcgatcgtca tcgatccgga
    22621 cgccacgaac gagtggttcg gcggctggac ggaccaggac tggaggacgc ccgcgccgca
    22681 ggagctcatc atctacgagg cgcacgtccg cgacctgacg atccatccgt cctccggcgt
    22741 gccccgcgat ctcgcggcga ccttcgcggg catccccgcg acggccggca cggggaccgg
    22801 gctcgaccac atgcagaggc tcggggccaa cgtcctggaa atgatgcccg tcaacgagtt
    22861 caacaacggc acgcgcgact acagttgggg ctacagcacg gtctatttct tcgcgcccga
    22921 ggcgtcgtac ggccgcgccc cgctcgaggg ctcgcagtac tacgagttca agagcatggt
    22981 caacgagctg caccgccggg gcttcgcggt catcctcgat ctcgtctaca accacgtcgg
    23041 ctacccgaac ctcttcagcc tgatcgacaa gaagtactac ttccgcctcg acccggactt
    23101 ccagtacagc aacttcagca agtgcggcaa cgacgtgcgc accgagaacc tcatggtccg
    23161 ccggttcctg gtcgacaacg tcgtctactg gatgaaggag caccacgtcg acggcttccg
    23221 gctcgacctc gccgagctga tcgacatgga cacgatgtac gccatggccg aggcggcgcg
    23281 caccgtgaac ccgaacgcgc tgctcatcag cgagccgtgg agcctccgcg gcgagaacaa
    23341 gtaccagctc cgcggaacgc cgtgggccgc ctggaacaac gagttccgct acgcggccaa
    23401 ggacttcgcc ctcggccggt acaaccgcga ctggctgcag aaaaagatcg tcggctccgt
    23461 ggacacctgg tccgcccacc cgatgcagag catcaactat atcgagagcc acgacgacat
    23521 ggcgctggtg gacgagttca gctcccgccc ggacaaggac ggccgcaacc tggacgagaa
    23581 ggacgtcgcc gccaaccggc tggccgcgac catcgtgttc acctcgctcg gcatcccgat
    23641 gatcaacgcc gggcaggaat ttctgcgcag caagcggggc atcagcaata cgttcgaccg
    23701 cggcgacgac gtcaacgccg tgcggtggac cgaccgggag cagccgatgg ccgccgaggc
    23761 gctggcctac tacgagggcc tcatgcggtt gcggcggagc ccggagggcg cggccttccg
    23821 cgtctccagc cgcccgcccg ccggctacta ccggtggatc acgccggaca acccgcagtc
    23881 gctgggctac atcgtcaacg ccccgcggcg ccacgagggc gcgggattca tcgtgctgct
    23941 caacgccggc ggcgagccgg tcccgttcga cgtgcccttt ccgcaaggcc gctggcgcct
    24001 ggtcggcgac ggcctccgcc tcgatccaaa gggcctgccg gacaccggcg tgcgggaggg
    24061 ggaccagtcc tacaccgtca acgtgccggg ccagcgggcg ctgatcttca tggacgggtt
    24121 ctgacggctg gccgggacgc gcaccctcca tgaaaaaaac cataccccct ggagggcgag
    24181 catccccgcg gggcattcgc tatcgcggat ttttccgcga actggacctt gcctcgatcc
    24241 ggccggccgg ctggctgcgc cggtggctcg agcgccagcg cgacggcctg accggccacc
    24301 tcgaacacgc gggttatccg ttcaacaccc ccggctggcg ccaggatccg atccgatgga
    24361 aaggccgcgc catccgctcg tggtggccct acgagcaggt ggcctactgg gtcgacggca
    24421 tgacccgcct cggcctgctg ctggaggatg cccggctgct gcgcaaggcg tcccgccagc
    24481 tcgaccgcgt gctcgcccgc ccggatcccg acggctacct cgggccgcag gcgctcaagc
    24541 gcaccacgcc ccagggccgc gcggaacgct ggccgcacgc cgtcctgttc cgggccctga
    24601 tggccggcct cccggcgggc cgccggcgcc ccatcctgcg ccaactgacc cgccattacc
    24661 tctccggcac ggcgaagttc agcgagtggc gcaacgtctg caacgtggag atcatgctct
    24721 ggctgcacgg acaaaccggc gaccgacgcc tgctcgatct cgcccgccgc agcctcgccg
    24781 gattccatcg ccgtcggccg ccggcgcccg ccagcctgcg cgccatgctg tccggcgcca
    24841 gggcccgcga ccacggcgtg aactactgcg agatatcgaa gctgcccgcc gtccttttcc
    24901 tgcacacggg caaccggacc gcgctgcgcg ccaccgtgaa cgcctggcgc aagctcgccc
    24961 gcgaccacgc gctcgcggac ggcgtgccca gctccaccga gcacttccgg ggccgcgccg
    25021 cgcacgccgg ccacgagacc tgcgtcatcg ccgacgctgc ctggagcctc ggctacctgc
    25081 tcatggccac cggcgacgcg gcgtacgcgg actggatcga gaataccgtc ttcaatgctg
    25141 cgcccggcgc gacgatgccg gacttcaagg ccctccagta tttctccagc cccaaccaga
    25201 tcgtcgccga cgcaagcgcc aaccaccacg aacacgggtt cggctgggct cacaaatcct
    25261 accggcccaa ccccgccacc gaatgctgcc ccggcaatgt cacgcggatc gtcccgaact
    25321 tcgccgcgcg catgtggatg gagcggcccg gccgcggcct ggccgcgacg ttctacgggc
    25381 cgtccgccgt gacgcaccgc ggctgcacga tctcggaaga aacggactat cccttcggcg
    25441 aaagcatccg cttcaccgtg cggactcgtc ggccctcgac gttttcgctt ttcctgcgca
    25501 tccccggctg gtgccgccgc ccgcggttgc ggatcaacgg cgaaccgttt gcaggcgcgc
    25561 taaagcgcgg gacgttcgtc gaggtgcgcc ggacattccg cgacggcgac cggctggaac
    25621 tggaactgcc gatggacatc cgtcgggagc gccagcccgg cggcgggctc tgtatccggc
    25681 gtgggccgct gctctatgcc ctgccgattc ccgcccgccg ccgccgcgac cggcacgaca
    25741 agcggtccag cgacgagttt cccgcctgga acctgcggcc cgccggcccc tggaattacg
    25801 cgctgccgaa gttttccaat gtttggaaaa aagcggaggt gatttttcca aacattggaa
    25861 aaaaacaggc cgaattttcc aaacattgga aaaaacgcgc cgaaattttc caatgtttgg
    25921 aaaatccctg ggccgagccg ccgatcgcga tccgggtccc cgccgtccgc gtgcccggct
    25981 ggcgcgcgcg ccgcctgaag gtgctgcgcg acaagtggca cggcgcacgg cgcggcgatt
    26041 tccggatgac accgcccctg ccgtccgcgc gtacgctggc gcgcgcgtcg cggaaggtcg
    26101 agtggatcac gctggtcccg tacggctgta cccagttgcg gatcgcgatt ttcccggcca
    26161 catgaaaacc gacaaggagc tctaccggca attgcgcggc gtgccgcgcg accggttgtg
    26221 gacggaggag gtgccgcggt tcaatagcgc ctcgccccgg gagcggatgg cccgggtggc
    26281 cgtgattcgc gcggtgggcg cggcgtttgc ctcgtccggt acgacggcgc agcgggccga
    26341 ggtgcggtcg tggctggtgg gactgttgcg cgatccaagc gaaaaaatac gccgctacgc
    26401 catgaccgcc ctgccgaagc tcggggccgg gccgggcgag gaggcggaac tgctttcgct
    26461 gctgcggacg acgggcgtcg agcgcgagcg gaaattcctg ggccggaccc tggataaaat
    26521 cggcgggcgg gccacgctgg aggccgtgaa ggcggccggg gggctgctcc cgcagaccga
    26581 gcagaaggtg aaggcccgcg ttgcgcgccg gcaaagcccc ggccgggtcc gaatggactg
    26641 ccgcctggag cggttcgaag ggctgcggat ccacctgcgc tgccggcgcg ggctggaaac
    26701 gatcgtccgg gacgaggcgg gagagcgact ggtgaccccg ggccgattcc gcggcatcga
    26761 gattcggcgc ggcctggtgg tcctcgcgcc cgcggcgccg ttttcgctgg cggacctgta
    26821 cgcgctgcgg tgtttcgacg ccgtcaactt cgtcctgggc acggctcccg ccgcgggcgg
    26881 cgcggaatac atcgaggcgc tggcctcggc cattgcgtcg cccctgtcgc ggcgactcct
    26941 gtccgccttt accgagggcg cgccgcgata ccgcctggaa tttattgccc ggggacatca
    27001 gcgcggggcg gtccggctcg tggcgaaccg ggcctatgcg ctgtgcccgg acatcctgaa
    27061 cgacccccgg cgcgcaacgt gggcggtgga tatccacccg caagggcgcg gaagcgttgt
    27121 ggaactgcgg ccgcggctga cgcccgatcc gcggttcatc tatcgccggg acgacgtggc
    27181 cgccgcgtct catccgccgc tggcggcgtg catggcgcgc ctggccgggc gcgcggaagg
    27241 ggagatcgtc tgggacccct tttgcggctc ggggttggaa ttgatcgagc gggcgctgcg
    27301 gggcggcgtg cgccaagtgt acgggacgga cgtgagcccg gaggccctgg ccatcgcccg
    27361 ggagaacttc gccgcggcca agctggagtc cgttccggcg cggttcacgt gttgcgactt
    27421 ccgcgagtct tccgcggtgg agtggctggg cccggacagc gtcacgctca tcatcacgaa
    27481 cccgcccatg ggccgtcgga tacgcattcc cgacctgcgc ggcctgttct cggatttctt
    27541 ccgcgttgcc ggccgcgcgc tgaaacccgg cggccggctc gtcttcgtca atcccctgcg
    27601 cctcgggccc gcggacgccg ccctgcaact tcagttccgg cagacggtcg acctgggcgg
    27661 attcgacgcc cggctcgaag tataccggaa gtcatgaaaa acccgcaacg cgaggcttga
    27721 cggatcccgt tttcgtctgc cattggtttc attgccatgc gggcgattca tgcggggctg
    27781 gtagtcggga tgctggcggc cggggtgacg gcccgcgcgc aatccgttac gccgttgttc
    27841 ctcggcgggc tccagccggg cgtcacgccg gtccatgcca tcgcggtgga taccaaccgc
    27901 ggacaggttc tggtcggcgg ccggttcagc tacctgtccg gcgcgcccgc ggccggcctt
    27961 gggatcctga acatggacgg ctcggtgtcg accgtgtacg tgtcgccacc gcccccgaca
    28021 ggcacgatcc tggcggcggc cttcgacgcg ccgcgcggcc gatacttctt cgcgggctcg
    28081 atcagcaacc cggcggcgga ccgggcctac ctcggcgtgg cgtatgtcac gcaaggtgtc
    28141 gggcgggtgg agtactgggc gggcacggag ccgggcgggc acatccgcgc gatggcgcag
    28201 cacgggaacc acctctgggt gggcggcgcg ttcacgagct ttgccggcgt ggcgcggcag
    28261 aacctggcgc agctctccgc gctgaccggc gagccgaccg acttcaacct gcccgcgagt
    28321 tcgacggtca cggttttttc cgtgctgggc gacgacacgc tgctggtcgg cggcgcattc
    28381 acgaatatcg gcaccgagcc gcgccagggc ctggcgcggc tgcatccgtc caccggtgcg
    28441 ctcgacacca actatgcagg ccggttccag ggcgggccgg tgtacgccat cgccccggcg
    28501 cgttccaacg tgtatgtcgg cgggctgttc accagcgtgg cgaaccaggc ctgcggtcgg
    28561 attgccaagc tggatgcgct gacgggggcg cgcgacctga ccgcaaccgg tggggctgtc
    28621 ggcgtgggcg cgatctacac gatcgtcccg gccggcaacg accatctgct cgtcggcggc
    28681 agtttcctga attacagcgg caacggccag cgctacttgg ccttggtcgg aaccaacctc
    28741 gacccgatcg ccgccttcac ggccggggtg aacgggatcg tccacgcggc cgccgccctt
    28801 tcttcgacca gcgcgctggt ctccgggcgg ttcacgcagt accaggacca tcccgcgctg
    28861 ggcctcgtgg aaatcctgtc ggagtcgtgg cggcggcatc cgatcttcag cgggtccgtc
    28921 ggagggccgg gcgaagtgta cgccctggcg gaactgccgg acgggacggt cgccgtgggc
    28981 gggacgttct acgacatcgg gggggagccg acccggaacc tcgcgcggct ctacccgaac
    29041 gggacgctgt gccaggcgtt cgtgaccaac gggccgaacg gccgggtgta cgccctggcg
    29101 ccggcggtac acggggcgga cacccagcgc gtgttctacg cgggcggcgc gttcaccagc
    29161 gtcggcgacc gcgcggtcgg gcgcctgacg cggtacgagt tcacggccgt ggcgggccag
    29221 tacgtcctga ccacgagcgt cctgccgcat tgcgacggag acgtgcgggc cctggcgacc
    29281 agcaccagcc atctctatgc cgggggctcg ttcgagagcg tggctggaat caccaatcac
    29341 gagcacatga tgggttgtgt tcatggctcc cttgcgccgg acccggcctt cgcgagccag
    29401 cccggctccg tattggtcgc ggtcctggtg gacacgaacc tcttcctggg cggctcgatc
    29461 tacaacgagg aggccatgtg gttcgacggg ttcgtgcgca aggtcagcgc cgcggacggc
    29521 gcgccgacgg ccggcttcaa tgcgccgacg cccaacgcgc cggtcgggac gctgtggctg
    29581 tccgacacga gcctctacgc gggcggcgat ttcacgatca tcggtccata ctcggccaac
    29641 tacctggcgc gcttccacac gggaaccggc gcccgggatg cgacgtacgc catgcacgcg
    29701 gacgcctcgg tctacgccct ggccggcacg acccagtccc tgtacgcagg cggctacttc
    29761 acgtcgttca ataccacgag tcgcctgcgc gtcgcccggt tcgatgcggc gagcgggcag
    29821 ttgcagtccg gctactccaa tattttcgac ggccaggtca acgcgctgct ctcgcagggc
    29881 tcgcgcctgt gggccggcgg ccagttcagc gccgccggcg ggcagccggc cgcgagcctg
    29941 gcgctcctgg gctggccgca gctcgcatac ctgcgccaga ccccggcagg attcgaactc
    30001 gcgatcgccg gcgcgcccgg ccagacaggc accgtggaac aggccgcgga tttgaacgcg
    30061 caggactgga gcgctcatgg ctcctatgcc ctgggccctg acgaaaacca ggttgtcgcc
    30121 gtggtcacca atccggcggc caaccgtttc tatcgcgcgc gcggggagta ggccgctact
    30181 tgcggagccg gatggcgatc cggccgtcct cgacgcggag gtcttcgatc ccggcggcga
    30241 aggtcttcca gaaatcctgg tccccgccgg cttcgtccag caggttgacg tccttcatcc
    30301 ccccgagcca cgcggcgggc agcgggatgc ccatgacgct caagccctgg aacacgacct
    30361 ccggtttttc gccggagagg cggatcttca agcccgcctt gacgcgcagg gtgcggccgc
    30421 cgaacacggg gaagtcctcg tccagcggga tcaaaagttt ggcgctcacc aggttttccg
    30481 acaggtcgat cgccagcttg tccgcgaggt cggtgttttt cgcgagcagg gcgttcagtt
    30541 ccttttcggt gatccggatc gtgcgttccg ctccctcctc gctgtacggc tcgggctcca
    30601 gcgccgcggc ggcggcgttg gtggccgtga aatccagccg ctccagcttg gcctcgagcg
    30661 cctgctcctc gcgcgcgttc agtgtcaccg gtctgaacgc cgacgggaaa tagatggcgc
    30721 ggaagaccag gaagctgatc gcgaccgtca gcacgacggc ggccagcacg atcagcagga
    30781 cctggaggca cccgaaccgc cttcccgctt tggatctctc gttcacggat tcccctttat
    30841 gtccttggcg ccttctgttc aacccgccag cagcgacgca aggtggttgc gcagctcggc
    30901 ggccaggtcg cgctgctcgt agcgggcgag ccgctcgcgc tggcccttca gcacggcccc
    30961 gcgcagcgac gcgtcgcgga ccagccggcc catcatttcg gccacgagat cgaaccgctt
    31021 ttcgcggaac agcacgcccg cgccgtcgag cgtgcccggc acggcggccg cggcgtaggc
    31081 cagcacgggg acatcgtgga ccaggctctc gatcaggggg atgcagaatc cctcgtgctc
    31141 gctcatgcac aggaaaagcc gcgccgactt gtagcaggcg ttcagctcgt cctgccggac
    31201 cgatccgatc agctcgacgt tctggagccg caggtcgcgc atcatcgtca gcagcagcgc
    31261 gtggtactgc tcgacaccgg cgaaggagcc cgcgtggatg aagcgcgagg cgggctcgac
    31321 atagcgctgg aagtagtgaa aggccgcgag gccatcttcg aggcgcttgt tgggcacgca
    31381 gcggccgacg aagaggacat tgaccaggcc atcgtcgtac ttcttgagca cgccgcggtc
    31441 cgcctcgccg cgcaggcgcg cgaggtcaag gacgagcggc aggacgccgg ccgggccgta
    31501 gccccaggac tccagctcgt ccgcgttgaa ccggctgtcg gcgaggacga cggaggccgc
    31561 gccggcgagg cgcttggcct gctcgcggcc ctttgcgagg tggtgggcga tctgttcctg
    31621 gatgccgcgg aaatagtcgg gcggggtgat gttgtggtag aggagcgcct tgtggcaggg
    31681 gagctcggcg aagaggtcgt tgaccggcga gccgatggaa aggtgcagga aggcgatgtc
    31741 gtccggccgg aaggactcct tcgcgccggc gacatcgcgg gcctcgccgc gcagctccgg
    31801 caggatgcgg cgggcctcgc agaagatgtc cgaatcgaaa ccccaggagc ggaagatgcc
    31861 gcgcaggacc agcgcctcgt tgctgatcgc gtcgccgtag ctgaacccgg cgacgagttg
    31921 gtggatggcg cgcttcatgc tgccggggat atagcacagg tgggcggcgc cgcgcaattg
    31981 tggcggcgcc ggccgcgagc cgcacgcgcc tgtccgtccg gaaaaaaacg accgggcgct
    32041 tcgcgcggcc ccggatccat gcgccggcgc agatatcaca gtttataaac tgtgatatct
    32101 gcggatttcg gggaggatgg cgcctggatc gggaggaagg aacgtccacg gattcgagcg
    32161 cgcggggtga ggccggcgat tacccgccat ccagcacggc caagaaacgc gcctggatcg
    32221 ccgtccggga gtattcgcgc aacacataac tccgcccggc cgcgcccatg cgctggcgga
    32281 gttcgggccg gcgcagcagc agcagcagtt cctgctcgaa gtccgggtag tgccggaacc
    32341 acaatccgcc gttgcttctc cggcactgcc agcgcaagac ctcgcccccc gcgtggacga
    32401 gcgccggcgc gccggccagc caggcctcca gcagcacgat gcccaggctt tcgttgaccg
    32461 acggatgaat gaacgccacg gcccccgcca tggcctcgcg cttttcgcgg tcggagacga
    32521 agccgacatc caggatgtac ggctccaatt ccgcaggggc ttcgatcggg ccgctgccgg
    32581 tgaacacgag ccgcacgtcc tcacccgtcc gctcgcggaa cgcgtgcagg tactcgcaca
    32641 gcagcggcgt gcctttgccc ccttcgcgcc ggccgctgta gatcacgtag gggcgatcca
    32701 atccatgcgc tcgcgcaaac gcggccggat cggcctcgaa aggctcgatg tccatcccga
    32761 ccaccgcgcc tttttcgccg gggagatgga acagccgccg ggccaggtcg cgctcgggct
    32821 cggtgttgaa ggcgaatccg cccaccgcgc cgaacagctc cttcatgatt tcgagatagg
    32881 cgaagggctc gtcgtgaagg caggggacca ggatggtttt ccgcgggtgg acttgcgccg
    32941 aaaaaaacga caggccaaac agataggggc cggccaggat gcggtcgtag tcggtgttgc
    33001 gcaggtgatc gagcagggcg cggctgttga cggagttccg gatccacgcg cgttcgtcct
    33061 ccggcgtgaa cgcgccgcgc tggctgatgg cctgctggat gcggaggaac gcggcgacat
    33121 cccggtcggc gtccacggga aagaaccgga catccaggtt tcccacgcgg cgcgccccgg
    33181 gcgggaccgt gttttcccac gtgaaatgac tggtggcgca ggtggtcagg atggtcacct
    33241 gccggccatc gtccgccgcc agctcggcga ggcgcttcag cagggtttcc gcgcccccca
    33301 ccgtccccgc ttccgaaaaa cggggactca ccacggcaat gctttgcgcg cgcgccatgg
    33361 acccactgta gagaaaagcc gcccctcccg ccaatgtgaa aatagctcgc catgcggcaa
    33421 aattagaaaa acgttccgcc taaacacacg aggcgcccat gaaccagcac gaaccgtggc
    33481 gaatctctcg ggggatggcg ttcgtggcgg cggtttttct tctggcgggg ccggcggcgg
    33541 ccgaggtgga ggtgacgttc gtgacgttga ttgcgaccaa tcccccggcg aatctgtact
    33601 cgccgcccag cggaggatct tcattctgcg tcggcttcag cgatacctgg tcggccggcg
    33661 cggcccgtga gatctacgtg gtgcccacca ccgccatcca aggaagcgcg aacaaggtca
    33721 ccctggtttt cccgacgaat acgggattcc ataccctccc gcggccgccg ggacggcctc
    33781 gggccggctc gtgctgggct ccccgcgccc gagcgtcacg cggttggagg attccacgga
    33841 tctcacggac tggagcccgg tcgccggcgc ggtgcaggcc tctcccgaag gccccgggtc
    33901 gcgtccattc ctggccagca tgccggcggg aaaaccgcgg cgcttttcct cgcccgctcc
    33961 gtggagtaag cgattcgatg tccgtccgag gccggcttcc cgaccgagcc cttttcaaga
    34021 tgcccacgcg cctggacgaa gccggggtcg cctgcaaacc gcattcggac cggaatattt
    34081 ttgagccggc gcttgtatcc tccccgcgaa tgatcataat gagccccggg taaacgcgga
    34141 ccgggccttg cggaatatcg cccggggatg gccggcggtg gacgactttc tggttttcga
    34201 aaacgtctcg aaaagtttcg gcaaggtgcg ggcggcggcg gacgtatcgc tgaccatccg
    34261 ccgcggggag tttttttccc tgctcggccc gagcggctgc ggcaagacca cgctgctgcg
    34321 gatcctggcc gggttcgaga gcccggacag cgggcgcgtc ctgctgaacg ggcgggacat
    34381 cacgcagctc gcgccgaacg agcgcaaggt caacaccatc ttccagagct acgccctctt
    34441 cccgcacctg accgtcgcgc agaacatcgc gttcggcccg cagatcgccg gctggccccg
    34501 cgcgcagatc cgcgaggagg tggacaagat gctccacctc gtccagctca cggaccaggc
    34561 ccacaagcgg cccgacctgc tgagcggcgg gcagaagcag cgcgtcgcca tcgcgcgcgc
    34621 gctgatcaac aagccccagg tgctgctgct ggacgagccg ctcgccgcgc tggacctgaa
    34681 gctgcgccag cggatgctga tcgagctgga cctgatccac gacgaggtcg gcatcacgtt
    34741 cgtgtacgtc acgcacgacc agggcgaggc catgagcctg agcgaccgca tcgcggtgat
    34801 ggacaagggc cgcatcgagc agatcggcac gccggcccag atctacgagg ccccgcgcac
    34861 gagcttcgtg gcggcgttca tcggcgacac gaatttcttc gaggggcagg tggtcgagct
    34921 gctcggcggc gactacagcc ggctgacggt cgagggcttc ccgtcggtcg agtgcttcaa
    34981 cgacaacaag ctgaacgtcg gcgacctggt ctacctgagc atccggccgg agaagttccg
    35041 catctcgcgg gagaggcccg agcccgcgcc gcacgtcaac gccgtccagg gccgcgtggc
    35101 ggacgtgatc tacttgggct cgctgacgaa gtactgggtg cgcgtgcagg actaccgcat
    35161 cgcagtctgg cggccggcga gccgcttcct gctggacgag aacccgatca cgtggaacga
    35221 cgaggtctgg atctcgtggc acggcgacga cagcttcatg ctcgagcggt acagcgagtc
    35281 ggacgagaag ctgatggagg cgccgccgga gcaggtgggc gaccccaacg gccacggggc
    35341 ggcgaagcca tgaccccggc gcgccgggcc cggtggacgg aggcgctggt gacgacgccg
    35401 tcgctggcct ggctgctggt gttcttcgtg gtgcccacgg tgatcatctt cgcgatcacg
    35461 ttcaagcccg tggacctcta cggcggcatc gggaagggct ggacgctgga cacgctgaag
    35521 agcctgttca atccgaacta cccggcgatc gtgtggcgga cggtctggct gagcctggcg
    35581 acgacggcgg tctgcctgat cctggccgtg ccgtgcggct actacgtcgc gcggtcctcc
    35641 gaccggtggc gccagatgct cctgctgctg atcatcctgc cgttctggac gagcttcctg
    35701 gtgcgcgtgt tcgcgtggaa ggtgctcctg cacccggagg gcccgctcaa gcacgcgctg
    35761 gcggccgtcg ggctgatcgg gccggacacg ctgctgctgt acaacgagaa ggcgatcctg
    35821 ctggtgatgg tctacacgtt cctgccgttc gcgatcctgc cgatctacgc cgcggcggag
    35881 aagttcgatt tccggctgct cgaggcggcg cgcgacctcg gggcgcgtcc gttgcgggcg
    35941 ttctggagcg tgttcgtgcc gggcatccgg cgggggctgc tgacggcggt cctggtggtc
    36001 ttcatcccgg cgctcgggtc gtacgtgatc ccggacatcg tcggcgggcc gaacagcgag
    36061 atgatcggga acaagatcgc gcagcgcacg ttcagcgacc gcaacctgcc gcacgccagc
    36121 gggctgtcgg cgttcctgac gctgggggtg ctgctcccgc tgctgctggt cctggccctg
    36181 cagcggaaga agggcgagga ggccccctcg atgcaggagg tctcgtgaag cgcagcgtca
    36241 tcccgctgct gacgaccctg ttcgtgctgg tgtttttcta cctgccgatc gtcgtgctgg
    36301 tggtcaactc gttcaacgcc tcgcggttcg gcgggacgtg ggagggctcg acgctggagt
    36361 ggtaccggat gctcttccgc gagaccagcg tctggcgcgc gctgcgcaac acgctgatca
    36421 tcgccgtcgg ggcgaccttc gcgtcggtgg tcctgggcac gaccgccgcc ttcgccctgc
    36481 accgctacca cacgcgcctg cagcggctgc actacaccct gatctacacg ccgctggtgg
    36541 tcccggagat cctgatgggc atgagcctgc tgctcttctt cgttgccgcg gggttcgagc
    36601 tggggctgtt cacgatcttc ctcgcccaca cgacgttctg catcagctac gtggccatgg
    36661 tcgtcctcgc ccggctgcag gacttcgact tctcgatcat cgaggccgcg caggacctgg
    36721 gggcggggtg gtggacgacg gcgtggcgcg tcctgctgcc gctgctggcg ccggggctcg
    36781 cggcgggggg gctgctggcc ttcacgctct cgatcgacga cttcgtcatc accttcttcg
    36841 tgtccgggcc gggctcgacg accctgcccg tgcgcatcta cagcatgatc aagcacagca
    36901 agaacatgcc catgatcaac gcgctgtccg cgatcctgct ggtcgtcact ttcgcggctg
    36961 tatggggcag ccagcggtta accaaggaga gccacgcatg aacgcacgta tcctgatccc
    37021 cgccctcgcg ctaggggcca ccctgctggc cggctgcggg gacaagacgc cgaccctgca
    37081 cttctacacg tgggccgact acatcaagcc ggaactcgtg cagcgcttcg agcaggagca
    37141 cgggtgccgc gtcatcatcg acaccttcga ctccaacgag gccatgtacg ccaagctcaa
    37201 ggcgggggcc acgggctacg acctgctcaa cccgagcagc tacatggtca agctgctcaa
    37261 ggaccagggc ctgctccagg acctcgaccg ctcgctcctg ccgaacctgg cgaacatcga
    37321 cccggagtac ctgcgcatcg cgatggaccc ggagatgaag cacagcgtgc cgtacatgct
    37381 cacgaacacg ggcatcggct acctggccag ccgcgtgccg gacttcgacc cgagctgggg
    37441 cgtcttcgac cgctccgacc tgcgcggccg gatgaccatg ctcaacgaca tgcgcgagac
    37501 gctgggcggc gccctgaagt tcctgggcta cagcctgaac tcgaccaacg agtacgagct
    37561 ggccgcggcc cgcgacgtcg tgatccgctg gaagaagaac ctggccaagt tcgagaacga
    37621 gcagtacaag accggcctgg cctccggcga gttcacgctg gtccacggct acaacggcga
    37681 catcctccag gtcatggagg ccaacgagga catcgccttc gccgtgccga aggaagggac
    37741 ctcgatctcg tgcgacgacc tggtcatccc gacgacggcg aaggaggtcg cgcacgcgca
    37801 cgcgttcatc aacttcctgc acaagcccga cgtcgccgcg gagaacacgg agttcatcgg
    37861 ctacctgtgc ccgaacagcg cggcctaccc gctgatgagc gaggagatcc ggaacgaccc
    37921 gtcgatcttc ctggccccgg agatcaaggc caagagcgag gtcatcgggg acgtcggcga
    37981 gtccctggcg ctgtacacga agatgtggga ccaggtcaag gcggcggagt gacggaagcc
    38041 gcaaggcgac agagtggcag agttgacgcg gccgcgccgg tttgtgtacc gtgggccgcg
    38101 taattccaag gaaaacaggg agaagaagga catgagcacg acgcatttca cgctgaagca
    38161 gtcggacatc ccgacccagt ggtacaacat cctggccgac ttcgcggagc cgctgccgcc
    38221 gccgctgcat cccggcacga agcagccggt gacgctgcag gacatgctgg cgatcttccc
    38281 cgagaacctg gtcatgcagg agatgtgccc ggaccggtgg gtggagatcc cggccgcggt
    38341 ccgcgacatc tacgccctgt ggcggccgac cccgctgctg cgcgccgtcc gcctggagca
    38401 ggcgctgcag acccccgcgc atatctacta caagtacgag ggcgtgagcc cggccggcag
    38461 ccacaagccg aacacggcgg tcgcgcaggc ctactacaac aagcaggccg gcacgcagcg
    38521 gctggcgacg gagaccggag ccggccagtg gggctcgtcg ctggccctcg cgtgcaagct
    38581 cttcgggctc gagtgcgtcg tgtacatggt caaggtcagc taccaccaga agccgtaccg
    38641 caagatgctc atgcacacct ggggcgccac ggtgcattcc agcccgacgc gccacacgga
    38701 gttcgggcgc aagatcctgg acaaggaccc cgagtgcccg ggcagcctcg gcatcgcgat
    38761 cagcgaggcc atcgaggaca ccgttcatca cccccacacc aagtactcct tgggcagcgt
    38821 gctgaaccac gtgtgcatgc accagaccat cgtggggcag gagtgcatga agcagatgga
    38881 gttggcgggc gaggagccgg acgtgctcat cggttgcgtc ggcgggggca gcaacttcgc
    38941 ggggctggtg ttcccgtacc tgcaacacaa gatcgccggg aagaaatcgt accgcgtcat
    39001 cgcggtcgag cccacgtcct gtcccaccct gaccaagggt gacctgaagt acgacttcgg
    39061 cgacacggcg ggcatgacgc cgctgctcat gatgtacacg ctggggcacg acttcatccc
    39121 gcccagcatc cacgccggcg gcctccgcta ccacggcatg gcgccgaccg tcagccacgc
    39181 gaagaagctg ggcctgatcg acgccgtcgc gtatccgcag accaagatct tcgaggcggc
    39241 cgtccagttc gcccagaccg agggcatcgt gcccgcgccg gaatccgcgc acgccgtggc
    39301 tgcgaccatt gacgaggccg tggcggcgcg cgaggagggg aagaagcggg tcatcctgtt
    39361 caacctctcg ggcaacggca tgctggacct ggcgtcgtac gacgcgtacc tcgccgggca
    39421 cctgaagtaa aaaagagatt aaccacgaaa tacacgaagg acgcgaaaag ggtgaacgtc
    39481 gcactccttt cgtgtattta gtgtatttcg tggttgtctt ttggagggtt tgttatgccc
    39541 cgcgccgcgc tgatcatttt cgcggttacc tacatcggcg tggcgttggg ccggctgccc
    39601 ggtctagccc tggaccgcac gggcatggcg ttgctcggcg gcatcgccat ggtcgtcgcc
    39661 ggcgtgctgt ccgaacgcga ggccctgctg gccatcgatt tcccgaccct cggcctgctg
    39721 tacgccctga tggtcctctc cgcccagctg cggctcgggg ggttctatac ccgggtcgcg
    39781 ctggcggttg cgcgcctgat ggatcggccg gcccggttcc tgctgatcct gatggccgtc
    39841 agcgcggccc tctccgccat cctcgtcaac gacatcatct gcctggcctt cacgccgatc
    39901 ctcgccgtct cgctgctgcg gagcgggctg aaccccgtcc cgtttctcat cggcctggcg
    39961 gtctccagca acatcggctc cgcggccacg atcatcggca acccgcagaa catgctgatc
    40021 gggcaggtcg gacggctgca cttcggccgg ttcctgctct ggtgcgggcc gccgtccctg
    40081 ctggcgctgc tggcggcctt cctcatcatc gcctggctct accgcggccg gtggcgccgc
    40141 ccggcgccgg ccgtgaaccc ggcatcggat tggccggcct ataacccgca ccaggcggcc
    40201 aagggggtgg cggcgaccgt gatcctcgtg gcgctgttct tcacgccgat cccccgggaa
    40261 ctgtccgcca tcgtcgtcgc cggcgtgctg ctgtgcagcc ggaagatgca gacgcgctgg
    40321 atcctcgacc tggtggactg gcacctgatc accctgttcg ccggcctgtt cgtggtgatc
    40381 cgcggcatcg aggccgcggg cctgccggac gcgctcctgg ccgcgctgca ggagcgaggc
    40441 gtggatatcc ggcagcccgg cctgctgacc ctcgtctccg ccgccttgag caacctggtc
    40501 agcaacgtcc ccgcgacgat gctgctggtg aagttcctgg accccgccga ccccgtgccg
    40561 tggtacgtca tggccctggc gatgacctac gcggggaacc tgctgacgat cggcagcatc
    40621 gccaacctca tcgtcatcga gcaggcgaaa ccctacggca tccacatcgg gttccgcgag
    40681 cacgcgcggg cgggcatccc cgtgacgatc atttccttgc tgatcgcgat cggctggatc
    40741 tggtggcggg cgtaagagaa ggaaaccgcg gaatacacag aagacacgaa agggggaatt
    40801 cttcctttcg tgtatttagt gtatttcgtg gcttttcttc cgtctctgtc ggacggcttc
    40861 gtagagcagg atcgtcgccg cggcggacac gttgagcgag tccgcctggc ccagcatggg
    40921 aatccgcacc tgcaggtccg cgtgctccat ccagaacggc ttgagcccgt actgctccga
    40981 tcccacgacg atcgccgtgc cgccggtcat gtccgcctgc gtgtaatcct gctcggcgtg
    41041 gggcgtcgcg gccaggatgc ggatgccgcg cgcgcgcaac caggccaatg cctcctccgg
    41101 cgacgcctcg accaccggca gggcaaacag cgtgccgatg ctcgcgcgca cgacgttggg
    41161 gttgttgatg tccgtcgtcg ggtcgcagac gagcaccgcg tccgccccgg cggagtccgc
    41221 cgagcgcagg agggttccga ggttgccggg tttctcgatg gactcggcga tcacgaggag
    41281 cggagaggtc ttctccagct tgaggtcggc cagtttccat cgaacctgcg gcccggtgac
    41341 gagcaggccc tcgggccggt cgcggtaggc catcttggcg aacaccggct cggcgcactc
    41401 gaacagctcc gcgccggctt ggcgacaggc ctcgaccagt tccggttcgt ggtggcccag
    41461 gtacagcggc gggcagtaaa agagggcgtt cggcttccag tggttggcca gggcgcggcg
    41521 cagttcgcgg tatccttcca cgacgaggag cccggcctcg tcgcggtgcg gccgccggcc
    41581 cagcttgacc acgtccttga cccggggatt ctggaggctg gtgatcttct gttccgcgct
    41641 catgccgggc ctgcttgacc tattcaacgc ttcgggtact ctggatgtcg taaccataaa
    41701 gggattcgcg ttgaaacgtc acgccaaatt gctgtccttc ctggcgctgc ccctgctggc
    41761 ggcggcggcc cgcgcggatg agaccgccga tttctcgcgc taccaggtga tcatggaccg
    41821 caagccgttc ggcgtcgcgc ccccggtccc cgtggcgccg ccgccgccgc tgacggccga
    41881 gcagtcgttt gcccggacgc tgcgcttgag cgggatgtgg cagcgcgggg acggcagcgt
    41941 ccgggtcgcc atcattgatg ccaatggaaa ccggaacctt tacttgagcg tgggcgagat
    42001 ggaagagggg atcgaactgg tgtccgtgga ccagcagagc gaggaggcgg tgctgcgcaa
    42061 ggggaccgag atggccgtgc tcaagatctc gtccggcgag gtccagccgc tgacgcagga
    42121 gcagcagcag cagcggctca cccaggacca ggcccagcgc atgtcctatg ccgagcggcg
    42181 caaggagcgc gagcgccagc gccagcagcc tccgcccccg ccgcccgagc cggtatacac
    42241 cggcgccgag ctggaaaaac atctccagga ataccagatg gaagtgatcc ggcagggcct
    42301 cccgccgctg ccccttccgc tcacgcccga gatggacgcg caactcgtgt ccgagggcgt
    42361 gctgccgccg atgcaatgac catcagggtt gcgtgatatc ggcgctggcg gaagaacccg
    42421 ccgtatcgga gaccgtaatc tgattatcgc cggcggtgag cctcgtgtag gtcacggagg
    42481 acccgctcga tgtgttgagc gtcccgttcg tgttcaggag gccccaggta tacgattcgg
    42541 tcccgccgga cgccgtgaag gtctgggtct gctggttggc ggtcaggttg gccgaggagg
    42601 ggctgatgtc cacctcgccc tcggtattgg tcggcgacgg attgatgacg gccttggcgg
    42661 acagcgtgga atactggctg ggctcgatcc aggttccgcg catcgtgccc tccgtgcccg
    42721 acccgttcag cgtgccggaa atcgtcacgg cctggccgac cgtggtgcgg ccttccagcg
    42781 tgaatgaggc cgttgtatcc acgaggttcc cgagcgtccc ccggaacacg atgccgttgt
    42841 tgtcaatggc ttccagctgg tcgccgttct ggcgcaggtt cagcagcgtg acctcggtgc
    42901 cgctgttggg cggcacgacc atctgggtgc cgttgctgcc gtagccctgg tagaagccgc
    42961 tgaagtcgag ggcgaccatg cgcaccacgc tgtccgcgga cccgacctcg cagccgcaca
    43021 gcgcgaaaat cagcagcggc gccagcgcca gggcccatcc gaaaacccgc catcccgttc
    43081 gtttcatggt gttctcccgt aatgactgcc cgggccggcg ttcaggccga cgcggtttcg
    43141 ttcctgccct tgtccgcctc gatggcgcgt tcgaccgtgg tgaggaagag atcgagtccg
    43201 gtggagggga tgatgatggt gtcccgccgg ccgccgacct cctccgtgat ccgcaggaac
    43261 ctgccccggg gattttcctt gaggtcgaag aagaactgtt tgcgttcgat ctggatccgc
    43321 tcgctcaaca acaccgtgtc caagtcgccc tccggttccc ctcccgcgaa gcctccctgc
    43381 cacgacgccc gccgggggcg ccgcgtccag ccgcaaacgc agatgtactc taatcacgcg
    43441 caggacgttg tcaacgccgg attggaccgc gccgcggtcc gttgcgttga gcgggaggcg
    43501 gttacggcag cgaggcctgg gcccggaagt acacctcggt gtctccgccg ctttccgccc
    43561 aggtcaggtc cgcgtcgttc cccgggacgc cggcgatcag gctgctccag gtctgtgttt
    43621 ccgacagggt cgtccggccg tcgatctcgt agagccgcgt gctcttggac gggaaagaca
    43681 cgtagcgggc cgggccgccg ttggtgacgc tcgtgatcgc caggtagaag gcgggatcgt
    43741 tggccggcgt gtcggcgacg tactcctcga cgtccggcac gaagtccccg tccgcatcgt
    43801 ccgtcggcgc gttggacacg gccggatcca ggccgaagag cgtctcccag tagtccggaa
    43861 tgctgtcgct atcggcgtcc gcgaccgcct gcagcgccgc gccggtggct agggcaaagg
    43921 tgaacccgga cgcgaaggag ccgacgccgc cggcatcggt cgtcgccggc acgcggccga
    43981 tgtaggcccg gctgccgaag ttggtcccgt ccgcctggta cacgtcgatg gagaaggctt
    44041 gcgacggcgc gccggtcagc gagccctgga cgtagaccca accgcccacg atcaggggct
    44101 gcccgagggc cgggctgttc tggagctggt tcgggccggc gtccgcgtcg agcggatcgt
    44161 tggcggtcgg cccgtcgccg ccgagatcga tgttgccccg cggctggttc gaatagacca
    44221 ggttgaccgc caggaggttg cccgagatca cgttgccgcc gatgaacatc ccgatgacgg
    44281 tgcccggcgc gttctcggcg tgaacgccgg tattctcgtt gctcacggcg gcggagcccg
    44341 ccggattcag cccgatgtag ttgccgtaga tcatgttccc ctgcgcgccg ccgtggaggt
    44401 acacgccggc cccgctgttg ccggaaatca cgttgcggcc cgcgcccagc gtcgcgccca
    44461 cctggttgct gatcccgctg tcgatacgca ccccgttgcc gtcgttgcca cggtccgtgc
    44521 cggcggcatc cacgcccacc aggtttcgca acacgagatt gcccacggcg ttcgatccgc
    44581 ggaggtaaat tccgtcgctc ttgctgtaga ccacggtgtt gccctcgccc gcggcggttc
    44641 cgccgatgcg gttggaccgg ttatcccaga ccaggatacc gacgttgctg ttcggcaggg
    44701 cgttggacgc gaaccccatg ccgatcacgt taccctggac cacgttgccg acggcccccg
    44761 tgccctccac cgtaatcccg ccgccccggc tgccgccaat gatgttcccg ccgccggccg
    44821 cgccgatgta attgctcggg ctattcagcg cgcgaacccc gtcaaagcca ttggaaacgg
    44881 cccacccggt gtggctggcc aggccgataa tgttgcccgc caggcggttg gagaacgccc
    44941 cgtagttaag gtgcacgccc cattccccgt tgcccgaaat cacgttgcgc tcggacgcgt
    45001 tcgtcccgcc cacgattacg ccgattgtcc cgtccagcgc gatgcccgaa atcgcgttag
    45061 ccagcttctg ggtgcccgat gcgtccgtgc cgatcaggtt gcccacgatc cggttgtcgc
    45121 gggtgttggt tccgctcatg gaaatccccg cggtgccgtt gccggagatc acctgcggcg
    45181 cggccgacgt ccccccgatc gtgttgccgt tcgcgttgtc gatcgccacg ccggagaggc
    45241 ggttcggcca agcggtcagg ccgtcgggcg aggtgcctat gaagttgccg accatgaggt
    45301 tattcgacgg gcccggcccg gcgaaccgca tgccaatgtc ggtgttggag gagatcacgt
    45361 tccgttcgcc ggccgcatag ccgccgatca ggttgccctg tccgccgccg aggtacacgc
    45421 cgttcccgtt gtggacgagg atattggccg ccaccgtatt gaagccgaag ccttgcaggc
    45481 tgacggccgc cacgttggtg aatcccgaga tgcggctccg ccgatactga agacgacgtc
    45541 gtccggaacg ccgccgttcg cgtgggcgtt ctcgagggcc tgccggagcg agccggggcc
    45601 gctgtccgcg ttggtgatca ccatgaaggt tgcggcttcg atcgcggccg cgcatgaggg
    45661 cggcggccag ggaaaaaagg gaacggaatc ggctggcccc tgtgcgcatg ctctcgttcc
    45721 tccgctctgc cgggaaacgc cggcgcgcct tccggacgaa aagggggctt tccgccgcga
    45781 aggtatccac atagcacagg gacaggcgtt ttcaaccgaa ccgggcgcag caagaaagag
    45841 cagccaatcc gggcgcgggc aatgtatcac agtttataaa ctgtgataca agggtaaagc
    45901 aggcgatatc cttaataaat aggatgaatc acgaccgcaa aatatcacag tttataaact
    45961 gtgatacatg cgtttttggc gccgcgccgt gaggagcgtg gggcgaccaa gtccgtccgc
    46021 taaaagcgca gcccggcgag gaaggtgacg gtagtcatct ccagctggat gaacttgttg
    46081 aacttctcgc ggagttcctc cacttcctcc gccgggcggt cctctgccat cacctcggcg
    46141 tccgccttca tgtgctgcac cccgacgccg aggagcagtt ccaggtcctt ggacatcggg
    46201 atgcccacga gggcgcgcgc ctcgaagtgg tcgtactcca ggtccacgtc cttggcgttc
    46261 cgcgaggaga tgccgaagtc ctccgtgctg ccgctgaaat cgagcagcgc gaaataggcc
    46321 tgcagcagca gctcgggggc ccgctgctcc gccgtcccgc cgccgaaccg gacatcgagc
    46381 ccggcgccca gctcgggcag gaccatgccc gtccagccct tgccgcggcc gccgaccacg
    46441 tactccctcg gcgggatttc atacagaacc acgccttggg ccggaccggc atcgatctcg
    46501 aaatagctga agaacgtgtt gagcccgagg ccaagggacg gggtgatctc caccgcgcgg
    46561 cccgccgtaa tcgaaacggg cgtggccagc ccgcgcatgg cgaaggagcc gccctgcatc
    46621 tccgcctcga agggcgcgcc ctcgggaata tgcatgtagt cgtaggtttc gccgttgtag
    46681 ctcacgtcct cgacgccgat gtaatagtct cgaaccgcct cgccgcgcac cgcgggagac
    46741 aggtaggaca ggttggcctg gagggtgaaa aagcgccatt gcttctcgaa gctgaaaccg
    46801 aaggtggcgt agccgccgtc gaacccgagc tcctcgagcg tgtaactctc ggcgaactga
    46861 tcgttctttc ccgggtcggt gatgtcgtag aaggggcgcg tcgtttcctg cacgtacccg
    46921 tcgagggtgg tgacttggcc cagcatcccc gccgcgtaag cgctcggctg cgcgggcgcc
    46981 gaggatcgac cccacagcgg cccggcccag ctggaggcgg acttccacag gaacaacagc
    47041 acggcggtcg tcagtatgcg gctcatcatg gcgcgttccc tttccgttag ggcgcggagc
    47101 gtcggcaaga acgcgcggca agtcaagaga aatgccggcg ggacggggct ttgacttttc
    47161 cggcgccggc ggctaaattg cgcggctttc catgaagatg ctgaccatca atgcctggct
    47221 gggcttgctg atcacggcgg tcgccctggt cgtcggtgga ttcgcgacgg tcaccagcat
    47281 ccgcggcgca cgcggcccgc gcgagcggcg cttcgtgcta tggcattgcg ccggcgcctg
    47341 gacggccatc ctgctgttgc tcgggctcgt cgtcgtcacg ccgtttccat ggaatctggc
    47401 ctggctgggc ctgtatttca tccacctccc gatcgtcatc taccggtttg cctcgaagca
    47461 ccagctcctg cgcgtcctgg aagcccggga atccgcccgc gagcgcgacg tggcgtgaac
    47521 ccggcgattt ccgggttgca gacgccgggc caacgaagca tgatgaggcc cgggataaag
    47581 gggaatgacg cttcgcagta aaaccctgct ggtgatgggc gcgtctttgg cgcttctctt
    47641 cgcggccgcg atcggggcct tcttccatat catgtcggcc cgcgacgtgc ccgaaggagg
    47701 cttggtgcac ctgctgccct ggctggtctg ggcggccacg gccggcgttc tcctgggcat
    47761 cctggtcgcc ctcctgcttc agaagcgggc gctctcgcgc gtggcgcggt tgtccgccgc
    47821 cgtgctgggc atccagaagc gcgccgatct ctccgcgcgg gtgccggtcg agggcgatga
    47881 cgagttgagc gccctcgcgc tcggcttgaa cgaaatgctg gcctccctcg aacgggccac
    47941 ggcccggcgc cgcgaggccg aggccgccca gcaggagagc gaagccctgt accgcctgct
    48001 gttcaaggag atgttcagcg ggtttgccct gcacgagatc ctctgcgacc cggacggcaa
    48061 gccctgcaac taccgtttcc tcgacgtcaa tcccgcgttc gagcggctga ccacgctccg
    48121 ccgggaggac gtggtcggca agaccgtgct ggatgtgctg ccccgcaccg agcccgcctg
    48181 gatcgagcgg tacggccgcg tcgcgctgac gggcgagccc gtccacttcg tcgactactc
    48241 ggccacgctg gaccggacgt tcgaggtcct gacctacagc ccccagcggg gccggttcgc
    48301 ggtgatcttc aacgatgtca cggaacagaa gctgcgcgaa caggagcagg ccaagatcca
    48361 gaaactcgaa tccctcggca tcctcgccgg cggcattgcc cacgacttca acaatgtcct
    48421 cgccgtcgtc ctcggcaacc tccacctgat ccatatgaac cagccgccgc tggacgagga
    48481 gacccgcacg ctgctgggcg aagccgagac ggcgtcgctg cgggccaagg ggctgaccca
    48541 gcaactgctg accttcgcca agggcggcct gcccgtgaag caggtctgcg aactggcgcc
    48601 cgtgctccgc gaggccgcca ccttcgccct gcgcggctcc acctgccgct cgcagctgca
    48661 ggtcgcgccc gatctctggt tcgtcgaggc cgatccgggc cagatcggcc aggtgatcca
    48721 gaacctggtg atcaacgcca gccaggccat gcccggcggc ggcgtcatcc agatccgcgc
    48781 ccgcaacgtc gtcgtggaga aaggcgccat ccacctgccg gagcccggcc gctacgtggc
    48841 gatcgtcgtg acggacaccg gcgtcggcat cccgccccag catctcgacc ggctgttcga
    48901 cccctacttc accaccaagc agaaggccag cgggctgggc ctggcgacca gcctgtccat
    48961 cgtccgcaag cacggggggc gcatcaccgt ggagtcgacg gtcaacgagg gctcgacctt
    49021 ccgcgtgtac ctgccggtca cggacaagac tccggcgccg gtcacggacc cggacgtcga
    49081 tacgctgccg cagggctcgg gccgcatcct ggtggtcgac gacgaggaac cggtgcgtcg
    49141 cctaacctcc cggatgttgt gccggatcgg ctacgagatc accgaggcgg gcagcggcga
    49201 ggaggccatc gcccgtttcg aggaggcccg gcaggcggga cgcccgttcc gggtggcggt
    49261 gctcgacctg accatccccg gcggcatggg cggcgtcgaa atcctcaagg aactccgcca
    49321 acgagacccg gacgtgaagg ccatcatcgc cagcgggtac gccaccgatc ccgtcatggc
    49381 ctcgtaccgg gagcacggct tcgtcggcgt ggtgaccaaa ccgttccgcc tggccgagct
    49441 ggcgcggacc attgcccagg ccgccggcgc gccggccagc ccgcccccga aatgagaagc
    49501 ccgggcgcgg gacgcccggg cctgatcgca caccgtcaag ggcggcctat tcctccgcct
    49561 cttcggactc cttctcttcc tccgcctcca ccggcgagat ggactccatg atgttgacga
    49621 actgccgttt cgactcgtcc gtcgccgtct ccttctcgat ggtgtacagc accacgaagg
    49681 cgcggttttc cgacgggccg aacaccgcga tggtgaactg cacctccgcc tcgctctcct
    49741 tctccacgcc gtcgccgtgc ttgagccaga atttcacgcc gtacttgttc gtgagctcct
    49801 cgtccgcgtc gtcgaactcg acgtccgaca gcatctgctc ggccgtcgcg gtcatctcgt
    49861 cttccaagtt gtccaggttc tcgacttccc acagcaccag gtacacggtc tcatcgccgg
    49921 agagcgcctc cagcgcgtcc tggtcgttga cctcggctgt ccagctcccg gggaagcgga
    49981 tgctgaaagc cggatccccc tcggggtact caaaggtttc ccccgcccgc gcggtgggca
    50041 cgaacgccgc cagcgcaacc gccatcacga tccaccactt gctcatgtcc cttccttttt
    50101 tttctgtttc aggccgggcc ggcgtccgtc accgcgccca gccgccctga cccgctatct
    50161 tcttcgtcgg cgaggcgtcg ctcccgtccg ccggcgcgct cacgagggag cggtaggggt
    50221 ccttgatctt gacccagtcc gggccggcat cctgcgcgag cgccttcccg cgccccgccg
    50281 gggccgcctt ggcggcccgg taggcctcca gggcctccgg catgtgctcc tccaggtcgc
    50341 gcgcgcgctc ctggcaggaa ggatgcgtgg acagcatgtt cagggcgcgg tcgatcccgc
    50401 tttcgtcctt cgccgcccgc tcccacagcc cgacggccgc gcggggatcg tagcccgcgc
    50461 gggccatgta catcagcccc acgcggtccg cctcgcgctc gtccttgcgg gagtacttgg
    50521 tcacgatcag cccctcgtag atcaggaagg cgccgccgag gatgtcctgc aggtcctcgt
    50581 cctccttgta ctccgcgtaa atcgccccgc ccaggagcag cagctcccac ggcaggttgc
    50641 gggtcatctc ctcggtggag tgccggcagt tgacatgcgc gatctcatgg gccaggacga
    50701 cggccaactc gtcctcgtca cggaccaggc cctcctcggg atcgtagagg ccggccagca
    50761 cgatgatctt gccgcccggc agggccatgg cgttgacctc gttggtgtgc agcaggatca
    50821 cttcgtacgg caggtccggc atgtgggaga ccgccgcgat gttccttgtg acgcgctcga
    50881 tttggcggac gcgccgggga tccttgttga tccccacctt ctcgtcccgc gcccatttct
    50941 tgatgtcctt atagaaatcg gtgccgagct cgatgtcgtc gtcaatggac cagaggttgt
    51001 tgaccttctt gccggtgacg gcatcgatgg tcgagcagga aaccaggccg ccgacaaagg
    51061 ccgacagcat caccgcgcgc agtactcttg tccatgcttt cattccgccc tccatgacat
    51121 gcgacgagcg cgggcgcgat ttattcaacc ggtcgggcgc tattctcctg tgccgaagca
    51181 cttccgtcaa ggcggtccag cctccgcgcc gcccagagcg cgtgttcgcg cagcccttcg
    51241 tcggggccgc tcatccagcg ctccagggcc ggacgcgcct cggcgcgccc gctgtttccc
    51301 agcgcgacga tggcgttgcg gatcagccgc gcccggccgg tccgctccag gggcgtcccg
    51361 ccgtaccgct cccggaaggc ccgctcgtcc aggtcgagca gctccgccag gcgaggcgcc
    51421 cagcggtccg gctcgaaccg tagaaacgga cgggatgcgg gccgcgaaaa tcgacggacc
    51481 catggacagg ccgcttggca ctcgtcgcaa ccgaagatcc agttgcccag ccgcgggcgc
    51541 agttcgggcg ggatcgcgcc gcggtgctcg atggtcaggt aggagaggca gcgccgggcg
    51601 tcgaggcgga acggctcggg cagcgcgccc gcggggcacg cctgcaagca gcgcgcgcag
    51661 cggccgcagg ggtggggctc ctccccggcg cagcttccgg gcgtcggcgc gtcgtactcg
    51721 agttcctggt cgagcagcag cacgcccagc aggatcatcg agcccatccg cgggtgaatg
    51781 agctgcgtgt tgcgccccac gaaacccagt ccggcgcggc ccgcgaaatg gcgctccaag
    51841 atcggtcccg tgtccgcgta cgcgcgccac gtcgcgcccg gtttctcgcg ctcgatgaac
    51901 cggcccagtt cctccaggcg cggcttcagc accgcgtggt aatcggggcc ccaggcgaag
    51961 gcggcaatgc gcccccggag cgggtcgagc gcgagggcat cctccggcca tgtcgtgaag
    52021 taggagcagc ccagcgcgac caccgatcgg gcgcccggaa ggacggcgtc cggctggccg
    52081 cgccgggccg ccgaacggcc cagccacgac atgtccgccg cgtatcccct tgccgccgac
    52141 tcgcgcagga aatcccggca ggcggggggg gccgccgggg cgatcccggc cagatcgaac
    52201 ccgagatcct gcgctgtctg tcgaatacgc gccgacaggt tcatcgtgaa aaacgccgtt
    52261 gttggattat cttaataacc gggcagaaag cacgcatgaa tacgaagcgt ggtatagcgg
    52321 gatgcgccga cggctgcggt gaacacgccg cgggcccgcg cgcgtggatc cggggggtgt
    52381 cgtgcgccgt ctccgggctc ctgacggccg gcggtttcgc ggcccatgcc gcgctcgccg
    52441 gcggcatccc ggccgccctg gccgccgagg cttcgtttcc tgccctcgcg cggtctttct
    52501 acgcggcggc catcctggcc ggcggatggc ccattgtgcc gcgcgccctg gcctcggtcc
    52561 gccggttgaa gccggacatg aacctgctga tgacgatcgc ggtcctcggc gccgtgctca
    52621 tcgggcagtg gttcgaggcc gccacggtga gcttcctctt cgcgttggcg ctccggctgg
    52681 agtcgtggag cgccgggcgg gcggagaagg ccgtgcatgc gctgatggac ctggccccgg
    52741 cgatggcccg tgtggtggat ctgcgggacg gcgcgatggc cgagcagcca gcggagtcgg
    52801 ttccggccgg ggcgagtgtg gccgttcggc ccggcgagcg catcccgctc gacggccttg
    52861 tgatcgacgg ggcgaccacc gtggacccgt cgccgatcac gggcgagtcc gcgcccgcgg
    52921 cgaagaaggc gggggatccc gtgtacgccg gcacgatcaa cgggcgcggg gcgttcacgt
    52981 tccgcgtcac gcggccggca gcggacacga cgctggccca tatcctgcgc ctggtcgagg
    53041 aagcccgcgc caaccgcgcg cccgtggaac agtgggtcga ccgcttcgcg cgcggctaca
    53101 cgccggttgt gttcggcctg gccctcgccg tgtgcctggt cccgccgctg ctcggggccg
    53161 gaccgtggag cgcctgggtt taccgggggc ttgtgctgct ggtgattgcc tgcccctgcg
    53221 cgctggtcat ctccacgccg gtgagcctgg tttcggctct ggcctcggcc gctcgcgccg
    53281 gcgtgctggt caagggaggc gcccatctcg aggcggcggc cgggatcaag gccgttgcgc
    53341 tcgacaagac cggcacgctg acggagggcc ggcccgtcgt gcagcgggtg atcccgctgg
    53401 acgggcacac gccggaggaa ctgctggcca ctgcggcggg actggaaacc cacagcgagc
    53461 atccgctggc gcaggccgtg ctggcctgcg cgcgccgcgc cggcgtcggc ccccgcccgg
    53521 cggtgaagta ccaggccctg cccggccgcg gcgccgaggc ggagatcggg gggcgccgct
    53581 tctggatcgg cagccaccgt ctcgtccacg acgagggcgg agagagcgag tcgctgcacc
    53641 gcgaactcct ggccctggaa gatgccgggc actccgtgat cgccgtcggc aacgagcgcc
    53701 acgtgtgcgg cgtcatcagc gtggcggagg gattacggcc gggcgcggcg gaggcggtcc
    53761 gcgaactgcg cgccctcggc gtcggacacg tgacggtgct gacgggcgac aacgagggca
    53821 cggccgccgc cgtctcccgg gccatcggcg cggacgactg ccgcgccgaa ctgctgccgc
    53881 aggacaagca cgccgccgtg cgcgaactgg ccgggcgttt cggcgcggtg gccatggccg
    53941 gcgacggcgt caacgacgcg ccggcgctcg cggcggccgc ggtgggcatc gccatgggcg
    54001 ccgccggatc ggacgccgcg ctggagacag ccgacgtggc gctgatgacc gacgacctct
    54061 cgcggctgcc ctggctgatc cggcatgcgc ggcggacggt gtccgtggtg cggcagaaca
    54121 tagcggtggc gctggggctc aagatcctgt tcatggccct ggcggcggcg ggccgcgcga
    54181 ccctctggat ggcgatcgcg gcggacatgg gcgcttcgct ccttgtcatc ttcaacggcc
    54241 tgcgcctgct gcggccggcg cggggcggcg agcagaagcc ggggccgccc ggggccttct
    54301 ccgccgaatg aggcggaggc gggaccgcgc ctgcctcgaa atcgacgccc ggcggatgta
    54361 tcacagttta taaactgtga tatccgggcg gcggccgatt ttttggcccg gcatcgaaga
    54421 acaactcgcg ggccttcgtc tcttggaggg cggcagcggc ttttggtctg gcgccgcctt
    54481 gccggttggg atacaagaaa aggccgaatc tctgggaaac gggggcaacc ttgaacagac
    54541 gggcgttcat gctggtggtg gtgctgtcgg cggccggcgc ggccgcgcag gggccgttgg
    54601 cggatcaacc cccggccggc agcctcccgc ccgtggagtc caatgccccg gcgttccgct
    54661 acgagggctt caagcgcatc gcgtgcgaca gcctctccaa cgtgatgctg cgggcggccc
    54721 attatttcgg cacgaacgag gtgctgtacc gcgtatcctt cgccgacgct ccgaccccgt
    54781 acttcttcgt gcggcttggg gatttcatcg gcgaatacga aatcgtcggc gcgggcggcc
    54841 ggcgcgggga cgagcacctg gtgctgcgga agggcgagga ggagatcatc ctgcctcggg
    54901 cgcgtaagta ctatgcctcg cccctgacgg tccgccggga aaagaaaaga gagtgagcgc
    54961 cgtggccccg ttctactggt cgccggaacg atacgccact tttcacgcct gcctccgccg
    55021 ctactactac cgctactacg cggaagctcc cgcgttccgc ccggcgcgcg acctgcggcc
    55081 gcggacggct tgggcggacg aatggctccg ccggggcgcc cacgagttcc tgcgcggggg
    55141 gctggacgag gaagccgcgg cggcgcggct gctccaggtc ctgcgcgatg atttcaaggc
    55201 ctcgcgccag cacggctacc gcgcccagcc gaagaccacg gacggcctct tcgagcatga
    55261 gtacgccatc tccgttccgg atgccgagtg gaaggacctc gcggaccggg ccgtggcgga
    55321 cctccgggct ttctgccgca ccgactacgc gcgggcgctg gccgccgtcc cggccgcagc
    55381 ccggctcgcg gtgggcgagt tcctgcggtt cgacctgctc ggcctgcccg tggatatacg
    55441 cccggatttc gccgcccgga cgacgaacgg cgtcgccctt catgcctggg gcccgcccgg
    55501 cgatggcgcg cggcccctgg cccgagcggc cctggcctgg ctggcggtcg gcctgtggaa
    55561 ggccgcgccg gaaagcgtcg aggtcgtgga atacgatttc cccgcggggc cgtcggccgc
    55621 ccaccggttc acggccgagg acctggaggc cgcgcgcgag ctggtgcatg acgccgccta
    55681 cgaaacccgc tacccgttgg ccgacccgga ccggaacgcg gcccgcgagc aggatttcga
    55741 tttcaccgac gacgaggcaa cctgccgcgc ctgtcccttc ctcaagctgt gcgcccgctg
    55801 gcgctgacgg gcccgctatc cgcgcaggcc cagccgccgg agaaccgccg cggtatcgga
    55861 caggtctgcg cagacatggt cggcgccggc cgcgcgaagt tcctcgacgc tgaatttccc
    55921 cgtcgccacg ccgacggacg ccgcgccgat acgggcggcc gcggcgatgt cgaagggcgt
    55981 gtcgccgacc agccagcagc ttcgcaggcg cgcgcccggc ggcagggcct gccgcacccg
    56041 ctggagcgcc agttgggcga tccgcccgcg gtcggcgtgt tcgtcgccgt aggcgccgag
    56101 gatgaaatgg ttgtgcaggt cgaactgccg cagcttgatc cgggcgcagc tttcgacgtt
    56161 gccggtgacc aggcccagga cgacgcgcgg atcgccggag agcgtctcca gcagcgcgcg
    56221 cacgccggga aacaacacca gttcggcccc cgcggccgcc cgctccagtt cctccggcag
    56281 ccgggccagg aacgtgcgcc gttcccgttc ggtcatcgca tggcggtgcg aggccatgac
    56341 ctgctggagc acgttgaggt cggtgttccc ggcgaaatta acgtacgcga tgtcgtccgg
    56401 ccagccgaac accgcctcca cggccttcgc gaaggaacgg cggcccgcgc cgcgcatgtc
    56461 gaggagggtc ccgtcgatgt cgaagaggac ggcgacccgc tcgcccggcg ccacgggggc
    56521 tggggacgca tccgtcatga agcggcgcgc gcgttgaaca tgcggtcgcc ggcgtcgccg
    56581 aggccgggga ggatgaaccc gtgttcgttg agccgctcgt cgatggcgca gacgtacagc
    56641 tgcatgtcgg gaaactgctg gtggacgcgg cggacaccct cgggcgacgc gagcatggcg
    56701 agcagtttca cggtcttgac gccccaggca tgcacggcct ccagcgcggc cacggcggag
    56761 ccgccggtcg cgagcatggg atcgagcacg agcgagacat ccaccgggtc gccttgcgga
    56821 agcttgcggt agtactcgat gggctgcagc gtcttctcgt cgcggtagaa ccccaggtgc
    56881 cagacctccg catcggggat gaggttcacg atggggtcca ccatgccgag cccggcgcgc
    56941 aggatgggca ccacgccgat gcgctgccgg acgacgcgtc cgcgcgcggg agccagcggg
    57001 gtctggacgg tcgtgtcctg gaggaccagg tcctgggtgg cctcgtaggc cagcagcgcg
    57061 gagagccgct gcagcaggcg gcggaactcc gccgggccgg tgtcccggtc gcgcagccgg
    57121 gccaggtgat gctgtacgag cgggtgtcga atctcgaata cgttccgcat ggcccgtcct
    57181 cccggccggc tccggatcag ccccccgtct tcttctccaa ctccgcgatg cgggcctcga
    57241 gggtcttgat cttcgcggcg tacttctcgt ccagcccctc gatcgcggtg accagcaggc
    57301 cgttgacctg gttctgctgc gaccacagcc ggtacgtgta gaacttcagc aggctccaga
    57361 ttgccttttt caggcggatc aggagggggg ccagatggcg gcggcgctcc cggatctcga
    57421 agtcggagat atccacgaaa accgcgtcgc gcaggcactg gaggtagaaa ccgaggaact
    57481 gctcctcgtt gcgcaggttg accaggttcg tgcgctcggc gcgcgcgatg cgggcgtccg
    57541 cgtagacgcc ctgttccatc ttgcggtccg cctcggcctg gatttcgcgg accaggcgat
    57601 ccgcatccac gcccgccgcg ccgatttcga acaggggctt ttccatgacg cgtattgtga
    57661 ggacgcggcc cgcttcctgt caacaccagc ccgcagtcgc ccgcgcgcct ttcccttgtg
    57721 acatgtttta caaaccatgt cacaaggcgc cgcggaggga aaaaaaggct agggagggtt
    57781 gtattccggc catgctgtat taccttcccg cttcggaggc ccgcattgaa cacgctcgtg
    57841 aaatggatgg tggcaggatt ggcgatcggc tgcctgggct ctgcgcaggc gtaggagaac
    57901 gcgaaaatga aaaccatgta cgacttcacc gtgaccgaca tcgacggcgc cgcggcgtcg
    57961 atgtcggaat accggggcaa ggcactgctc ctggtcaacg tcgccagccg gtgcggattc
    58021 acgccgcagt acgagggcct gcagaagctg tacgagcgct accgggacgc ggggctcgtc
    58081 gtcctgggct ttcccgccaa caacttcatg aaccaggagc ccggcagcga cgccgagatc
    58141 aaggagttct gctcgacccg gttccacgtg accttcccga tgttctccaa gctctccgtc
    58201 aggggcgacg acatccaccc gctctaccgc ttcctgacct cggaagagga gaacccgggg
    58261 ttcggcggcg cgatctcgtg gaacttcaat aagttcctgg tcgggcgcga cggcaaggtc
    58321 gccgcccgct tcggctcccg ggccgcgccc gaaagccgcg aggtcatgga ggccgtcgaa
    58381 aaagccctcg ggtcgccggc cccatgaaca agacggccat ttttcttgcc ttggcgttca
    58441 tggcgttggg cgggccgctt gcgcgcggaa ccgccatgcc aaaattcccc gcccgcgtgg
    58501 acgcggaggg cactccactc gccctccgaa gcaccgggat cttcaaggcg cgcgtgttct
    58561 tttccgttta cgcggccgcg ctgtacttgg aggaaggcgt ttcgtcggcc cgggttctcg
    58621 aggacgttcc aaagcgcctg gaactgcatt acttttacag catcccggcc tcggtcatgg
    58681 tcgaggaagg cggcagtgcc ctcgcggcta atctttcacc ggaccagcgg gagctcttgc
    58741 gggaacggat tttgcaactc aatgcggcgt atagagatgt gaagcctgga gaacgttatg
    58801 cgctgacgta tgtgcccggc cgtggcacgg tcttgagcct gaatggagaa gagatttgcc
    58861 ggattgatgg ggccgatttc gcggcagcct acttccgtgt ctggctgggc gaaaaaccgt
    58921 cgagccgggc cttgaaaaag gccttgctgg gtccagccgg gtaggcctgc tcacagggtg
    58981 gtctgcacgc ttggtcaagg gcttgacttg gagggcggct tcctctactg tctgcgcgcg
    59041 ttcaagacct atcggcccct acgggcgact tgaaaatcct gaagaaactg aagtttctga
    59101 acaacaagaa ggagtcaagc atggccaaga aatacgtata cagtttcggc ggcggcaagg
    59161 ccgacggaaa cgagtcgatg aagaacctgc tgggcggcaa gggcgccaac ctggccgaga
    59221 tggccgggca cccgaagctg cgcctgcccg tgcccccggg cttcacgatc accaccgacg
    59281 tctgcaccca ctactacgca aataaaaaga cgtacccgaa ggagctgaag gcccaggtcg
    59341 ccgccgcgct ggcgacggtc gagaagctga tgggcaagaa gttcggcgac gtcaagaacc
    59401 cgctgctcct ctccgtccgt tcgggcgcgc gccgctcgat gcccggcatg atggacaccg
    59461 tcctgaacgt cggcctgaac gacgagacga tccagggcct gatcgccgcg acgaagaacc
    59521 cgcgcttcgc ctacgacgcc taccgccggc tgatcatgat gtactccgac gtggtgatgg
    59581 aaaaggccgc cggcatcgag cccaagggcg gcaagggcat ccgcaaggtc ctcgacgaac
    59641 acctcgagca cgtcaaggaa tcgaagggct acaaggaaga cacccagctg acggccgagg
    59701 acctccaaca gatcgtcgtg gacttcaaga agacggtcaa ggacgtgctg ggcaagccgt
    59761 tccccgagga cccgatcgcc cagctgtggg gcggcatcgg cgccgtgttc atgagctgga
    59821 acggcaagcg cgcggtggag taccgccgca tcgagaagat cccggacgag tggggcaccg
    59881 cggtcaacgt gcagtccatg gtgttcggca acatgggcga tgactgcgcc acgggggtgg
    59941 ccttcacccg caacccgggc aacggcgagg cccagttcta tggcgagtac ctcgtcaacg
    60001 cgcagggcga ggacgtggtc gccggcatcc gcacgccggc ccccgtgaac gagtattcca
    60061 agaacgacca gagcgaaaac ctgccgacgc tcgagaagct catgccccgg tgctacaagg
    60121 agctcttctc catccagaag cgcttggaga agcactacaa ggacatgcag gacatcgagt
    60181 tcaccatcga gaagggcacg ctcttcatgc tccagtgccg catcggtaag cgcaacggcg
    60241 tcgccgccgt ccgcatcgcc acggagatgc agaaggagaa gctgatcaac gcccagaccg
    60301 cgatcatgcg cgtggcgccg gcccagctcg tcgaactgct gctccccatg ctcgacccga
    60361 aggccgagct ggccacgcac gccatcgcca agggccttcc ggccgggccg ggcggcgcga
    60421 tcgggcgcgt ggtgttcacc gccgccgacg cggtcgagtg ggcctcccgc ggggagaagg
    60481 tcatcctcgt ccgcgaggag acctcgcccg aggacgtgga cggcatgcac aaggcgcagg
    60541 ccatcctgac cagcaagggc ggcatgacct cccacgcggc gctcgtcgcc cgcggctggg
    60601 gcaagtgctg cgtggtcggc tgctccgaca tggagatcgc cgacgacgac aagtcgttca
    60661 agacccggag cgggacggtg gtgaaggaag gcgagtggat cagcctgaac ggcaccaagg
    60721 gcctcgtcta cgccggccag ctcccgctgg tggcggtcga cctggatcac aacaagtcct
    60781 acaccgacct gatgaagctc gtggaccggt tccgcgtcct gaaggtccgc accaacgcgg
    60841 acacgccgaa ggattccgcg caggccatca agttcggcgc ggagggcatc ggcctgttcc
    60901 gcaccgagca catgttctac ggcgagggca gcgacaagcc gctgttcctg ctgcgcaaga
    60961 tgatcatgtc caagacgctg gccgagcgcc gccaggccct cgacgagctc ttcccgttcg
    61021 tgaagagcga catgaaggcg acgatggaag tgatggccgg ccgcccggtg accatccgcc
    61081 tgctcgatcc cccgctgcac gagttcgtcc cgcatgacga ggacaagctc caggcgctgg
    61141 ccaacgagct gaaggtggac atgagcgagc tcaacaagcg cgccgacgcg ctccgggaaa
    61201 acaacccgat gctcggccac cgcggcgtgc gcctcggcgt cacgtacccg gaagtcaccg
    61261 agatgcaggt ccgggcgatc ctagaagccg cggccgagct gatcaaggcc ggcaagaagg
    61321 cgttcccgga aatcatgatc cccgtgacct gcgcggtcag cgagctcgat caccagaagg
    61381 tgatcgtgga tcgggtgtac ggcgaggtct gcaggaagtt cggcctcaag aagctcgagt
    61441 acatgtacgg cacgatgatc gagatcccgc gggcggcgct gcgggcgggc cagatggcgg
    61501 agacggccga gttcttcagc ttcggcacga acgacctgac ccagatgggc ttcggcttca
    61561 gccgcgacga catcggctcg ttcctgcctg attacctcaa cggcaagctg ctcccggacg
    61621 acccgttcca gacgatagac caggacggca tcggcgagct gatcaagctg ggcatcgagc
    61681 gcggccgcaa gacccgcaag aacctgaagg tgggcatttg cggcgagcac ggcggcgacg
    61741 cgaccagcgt cgagttctgt caccgcgtcg ggatgagcta cgtgagctgc tcgcccttcc
    61801 gcgtgcccat cgcgcggctg gcggcggccc aggcggtcat ccgcgggaag aagtaaaggc
    61861 cgcagcgagt tcggcgaaac cccggcgtcc gcgccggggt tttgctttcc tattgaaatc
    61921 ctcctctccc tccttctcct cctctccctc tcgcattcga gggcaagaag gagtaggaga
    61981 gggagaaaga gaaggagaaa agaggcggca tggttcataa aacctccaag cagtacgtgt
    62041 tcgcgttcgg gcccggcgcg aagaacacgg agggggacgc ctcgatgcgc gcggtcctcg
    62101 gcctgcgcgg cgcgaacctg gcggagctgt cgcggctggg cgtcgccgtg ccgcccggct
    62161 tcaccatcgc caccgaggtc tgcgcctact tcacccggca cggcggccag ttgccgtcgg
    62221 gcctcctgga ccaggtggac gcggcggtcc gcaagctgga atccctcacg ggcatgaagt
    62281 tcggcgaggc gcgaaacccg ctgaccgtcg cggtccggtg cggcgccggg gtcgtgctcc
    62341 ccgggctcat ggagaccgtg ctgaacctcg ggctcaacga ccagaccgtc ccggcgttct
    62401 gcgagcagac cggcaacgcc cgcgccggat gggacgggta ccgcctgttc atggaacggt
    62461 tcggggccgt cgtgatcgac gcggaggccg ggctctccca gtccgatttc gacgcggagc
    62521 gatcgaagct caaggacaag tacggcctcg cgggggacgc cgacctttcg gccggacacc
    62581 tccgcgaact gtgcgatatc tacaagcgcc tctttttcca gaagacccgc cggcccttcc
    62641 cgcaggatcc ccgcgagcaa ctccggatga cgatcgcggc gggcctgcgc tcctggacct
    62701 ccgggcgcgc cgagcattac cggcaggccc acaaggtggc gggccttctc ggcgcggccg
    62761 tgaacgtgat cgcgatggtc tatgggagcc tggacgacga gtccggctcc ggcatcgtct
    62821 cgtcgcgcga cggcaagacg ggatcgagcc ggccggtggg cgtcttccgg gtcggcgcgc
    62881 agggcatcgg gctgtccgcc gcggcgtccg ggttgaagga cattcacgac atgccgaagg
    62941 aaaaaccggc cgcgtggaaa aaaacgtacg agcagctggc ggagctcctt cacaagctcg
    63001 aaggccatta ccggtacccg caggaggtcg agttcgccgt cgagcggggc aagctctgga
    63061 tcctgcaggc gcagaacgcc cagcgctcgg gccgggcggc ggtgcggtgg gcgctggaaa
    63121 tggcctccgg ccaggacgcg gtcagcggca agccgctgcc ccgcgtattg aaggccgagg
    63181 aggcgctgct gaccctcggc gcgccggatc tcgacacgtt tctcttcccg ctcttcgacg
    63241 cggcggcgga gcggcaggcg atcctgctgg cccgcggcca gcccctggcg ccgggcgcgg
    63301 catcggggcg gatcgtcttc tcgctccaga aggccggcga tctcctgcgc aaggatccgg
    63361 cggcccgcct ggtcctcgtt tgccgggaac tcggcgaggc ggaccgcgcc aatctccggc
    63421 gcgtgcaagg ggtgctggcc gtcggggccg gcctggattc gacgctggcc gccgcggcgc
    63481 gcgcccaggg ccggggcg
//
LOCUS       DAOOTK010000005        62767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_373_length_62767_cov_5.947235, whole genome shotgun sequence.
ACCESSION   DAOOTK010000005 DAOOTK010000000
VERSION     DAOOTK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 62767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62767
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_62767_cov_5.947235"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2382)
                     /gene="smc"
                     /locus_tag="PK634_01390"
     CDS             complement(<1..2382)
                     /gene="smc"
                     /locus_tag="PK634_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOW96418.1"
                     /translation="MYLKHLELIGFKSFADRTKLDFEPGMTAIVGPNGCGKSNIADSI
                     RWVLGEQSAKALRGAKMEDCIFNGADTHKPMGMAEVSMTFADCEKVLGTEYNEVTVTR
                     RVFRDGEGSYFINKTPCRLKDIQRLFMDTGIGTDSYSILEQGRIDQILSSRPEDRREV
                     FEEASGITKFKADKKEAIRKLEHTEANLLRLADIIREVRRQIISLQRQAGKARRYQVI
                     QEQLRGLDLFATRDRMQAYDLDLAQLEKRVAGFAEQEEALRAHMAETERQASENRAAL
                     SAAEQAIAQAMEASMRARTDLDRARELIQVNEDRIRELQTLSDRDRHDADEARQRLDQ
                     HRASLEELGRQLTDAVAARDAAERDLQARQADLAAHEDKLSATRQFLHDLSTEMIDIE
                     SRTSRLQNELYDLEASERTATLRRERLAAEQSELRHAVDIYAARQEEMNGSLGALRDE
                     VGSLQGRHGERAAAKDSAARQLADTLKSLADLKARLAAREAQVEMLDQPEARLDGLPS
                     GARALLDPPADWNLRAGAVLGALTDHLQPDPAYRVALEAALRTGLDALAVADESTAAD
                     LLSQLERRAAGAARLLPLAAAAPGPDPWPDGPGRPLAERVRCAPEWRPAVDRLLRGVR
                     VVDVLPAPGAAIPAGATLVTPGGAVRRGNGLLEFWMPGAHEITPLARRQYLEDWTREL
                     ADLRRQHTEGELQAARLRDDIAAAEAEETGLRRQLDERRHALAQREGEQHIIAQEADQ
                     ARQRADTVAWEWQSLQEQTSSGAERKNAIHAELERYRARQAEIRAGTASKNDEL"
     gene            2597..2890
                     /locus_tag="PK634_01395"
     CDS             2597..2890
                     /locus_tag="PK634_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOW96419.1"
                     /translation="MYVTLVHVRVKKEAVPSFIQACRANHEASTSEPGNRRFDVLQLK
                     EDPTRFVLYEAYASKEDAAAHKNTPHYAAWRDAVAGWMAEPRKGVVYDGLFPA"
     gene            complement(2895..4097)
                     /locus_tag="PK634_01400"
     CDS             complement(2895..4097)
                     /locus_tag="PK634_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOW96420.1"
                     /translation="MKARPVGSAILALALAAALPAAADLAQDNTAFAVDLYRALAPAP
                     GNLFFSPYSISTALAMTYAGARGETEKQMAATLRFSLGQEELHPAFADLSARLARTQK
                     EGQVQWTAANSLWPQQQYPFLPEYVALLEKEYGVSVTPLDFGEPESVRARINGWVEEK
                     TREKIRNLIQPGFITSMTRLVLVNAIYFKGKWAAPFDAAATRDAPFHLSADQTVQAPM
                     MIRKLKCAYAHFPELDLAELPYAGGDCAMLILLPATVDGLRTLEQGLTADRLGAWRDE
                     MSDRDVLVTLPRFTMTSAFRLNDTLSAMGMPDAFSDTLANFAGMDGRPDWLYIGAVLH
                     KAFVEVNEEGTEAAAATAVVMQPRAMAPSRPVLFTADHPFLFLIVEKQTGSLLFMGRL
                     SDPTKAGE"
     gene            complement(4114..5181)
                     /locus_tag="PK634_01405"
     CDS             complement(4114..5181)
                     /locus_tag="PK634_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW96421.1"
                     /translation="MALTKTGGQYREELKALAAGLPFKVITRPEFRRPAGCPPPVRQK
                     RTYMSPDSNMQAVGRFLIERGTVELLTPESTLALFTEIHWCATQIRLLSRRHMKSSAQ
                     WREAGVAARKLISQIEAAEEELFIANRRLVVSCIKPYFWIGQVWLGDFLQEGSKALSN
                     AIRKFDFTRGTPFYVYAQKAVQNRLRNYFRDHVRSGSIGMRPSREMLALKEILDAWPK
                     DRGEPPDDALLANLTGMTEERVAKIRPYVRQLEHQPGPPVSLDAMFADNESNLYDIVE
                     DSEAEEASHAAQRSEIWTAVERLPARSKLIMRLRFIEGRTLEETGAMLNLTRARIKQI
                     QDDALRKLRQMLRRGVVNPRM"
     gene            complement(5251..7185)
                     /locus_tag="PK634_01410"
     CDS             complement(5251..7185)
                     /locus_tag="PK634_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOW96422.1"
                     /translation="MRRNAPSKAFILAAGFGTRMLPLSRDLPKPMMPLWGRPILEHVL
                     LLLRNWGVRDVLINAHHRPGELVRWAAGRPVAGLRVNFSFEPEILGTGGALRRAAWFL
                     DKRPFWLINADVAADLDPAPLLRAFARARPLAALWLHPSLGPRTVSMENGLVTSFHAP
                     VPGSEGTFTFCGLHLLSPRILDYLPAEGFAGIVPAYERATKRGERIAGVCVPDSFWAD
                     LGTPRQYLDAHAAVRQARLDGKPGGRLFARMPRPAGARVQGWAAIPCSVRIAAGCRIE
                     NAVAWEGARLGPRTRITDAIVGRDAVVNEPVRFLAGRGDRVLDDAEQKALQQYGWPAA
                     QATVLPFGPRGSARAFARIRRGSQSLILVRYDSARAENALQVPHTRFLARFGFPVPAV
                     RLDQPAARLALLEDAGDRCLRDVVLCSRPNRIELLYERILDQAARLHETGTRRAQQEH
                     LPLMQPFTPKLYRWERELFVTHFLRNAGRASAGEIRRIRRELAGIAARLNRAPRVLLH
                     RDLQSSNILLKRGLPVFIDFQGMRFGPAAYDLASLLCDPYVALPEPLQEKLLRHYARR
                     RPSDPSAIRLFRPAAVERLAQALGAYGQLSAQPGLESFARFIPPALRLLRRALGRSPA
                     LPVLAAVTERLLSRESSGRR"
     gene            7307..8347
                     /gene="purM"
                     /locus_tag="PK634_01415"
     CDS             7307..8347
                     /gene="purM"
                     /locus_tag="PK634_01415"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOW96423.1"
                     /translation="MGKTSKKGAAYTRAGVDIDAKMNALRAVRAEIRGTFTRGVVGDI
                     GHFGGLFRAPGRDRLLVSSTDGVGTKLKVACLAGRHDTVGEDLVNHCVNDILVQGAEP
                     LFFLDYVGMSRMNPAVFRQVVRGLCRACRRNGCALIGGETAELPNLYPDGEYDLVGTI
                     VGSVPRRELITGAGIRPGDVLIGLPSSGLHTNGYSLARRVILEEAGLKLRDRLPGTRR
                     TAADALLAVHRSYLKPVRALKRAVPIRGMAHITGGGFPDNIARILPPDVCAVVDRALW
                     RPPPLFQFIQEAGRVDRDEMYRVFNMGVGLVIFVREARAVEAVEVLRAAGERAVAIGQ
                     VEKGRRGVILVN"
     gene            8357..9985
                     /gene="purH"
                     /locus_tag="PK634_01420"
     CDS             8357..9985
                     /gene="purH"
                     /locus_tag="PK634_01420"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOW96424.1"
                     /translation="MKIQRALISVSDKTGIVEFARALTAMGVELLSTGGTAKSLREAG
                     VAVREVSDFTGFPEMLDGRVKTLHPKIHAGLLYLRGQPDHEATMTKHGLLPIDLVCVN
                     LYPFESTVARDGVPLWEAVEQIDIGGPSMLRSAAKNHAAVTVVTDPSDYARVLDQMRG
                     HGGDTTPALRMELAQKVFARTAAYDAAISRFLANRLDASTPPPLVLSAGDPVKLRYGE
                     NPHQEALFYREPDPRAACIARVEILHGKDMSYNNYLDGDAALEAVRELCGAPAAAVIK
                     HSNPCGYATGRTLAAALAAAWDGDPVSSFGSVIAVTVPFSLEAAQVLKGRFVEALIAP
                     DYEPDALEFLKAKSKDLRILKLNRPLTPPVRDRVLRQVHGGILVQDRDVMEMEQWVIP
                     TEALFPEEKRALAEFGIRACKHIKSNAIAIVREYEPGQFALLGMGAGQPNRVDALRKL
                     AVAKARENIALLAKHASTYGQSPKEFECRVMGECALVSDAFFPFADNIEAAAEAGIRY
                     IVQPGGSKKDEEVIAACDKYGIAMAVTGVRHFRH"
     gene            complement(10009..10569)
                     /locus_tag="PK634_01425"
     CDS             complement(10009..10569)
                     /locus_tag="PK634_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96425.1"
                     /translation="MKRPILCGLILGLLASVACARDNIEITATKKTLDARKGNEQDLW
                     RGTSRSVEKQICYSIELKNWSPATPTDVKVEWLILVEGAGGRLFPGTFGSKSISLALG
                     QPVVVETEAFKLEGREWTGSLNPGTVEDDIAGYGIRVLSTDGTVLAEKYDPLSAKANV
                     GWNLLDESSGEKARKLPPRLRPLKAP"
     gene            complement(10597..11355)
                     /locus_tag="PK634_01430"
     CDS             complement(10597..11355)
                     /locus_tag="PK634_01430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOW96426.1"
                     /translation="MMRLQKYLAQCGIGSRRACEQLIADGRIRVNGQVVRRLGTTIDP
                     TRDRVEADGRAVRPEAPVVIAVNKPIGLICTSHDPQGRPTVVGALAGTRSVSDDPWPA
                     GRLYTIGRLDADSEGLLLLTNDGELAHRLMHPRHHVPRVYRVWVRGRPSAEQLRQFIE
                     GVEYEGETLRAQEVRVLQARSETTCCRVILKEGRKRQIRRMFEVLGLPVARLRRTDIG
                     PLSLGRLPIGGWRFLRPEEMAGLRLDWTATSPRN"
     gene            complement(11352..12206)
                     /locus_tag="PK634_01435"
     CDS             complement(11352..12206)
                     /locus_tag="PK634_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOW96427.1"
                     /translation="MRTVEDIEIRFPGSLAEALAWQADEKTRGRPLAGGTDLMVQWES
                     GVAAIPERAVSLWGLKELKGIRETPRGIEIGAMTTHTELRSSPLVREKLPALAAAAAT
                     VGGRQIQNRGTIAGNVANASPAGDLAPTLLITDGLATAASASGRRDIPLAKLFTGYRK
                     LDVRADELLVSFTLPALPAGAREKFHKIGTRAAQAISKVMGACRVRVQNGTIVSLAVS
                     LGSVAPVPVRLPELEQWIAGRRVDLNLLEEVERRASAEVHPIDDIRSTATYRKWVSGR
                     LVRALLEA"
     gene            complement(12203..12682)
                     /locus_tag="PK634_01440"
     CDS             complement(12203..12682)
                     /locus_tag="PK634_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOW96428.1"
                     /translation="MSTVKRTWVVNGERWTVDLPPGRRLLDALRNDLGLKGTKEGCGE
                     GECGACTVLLNGEPVTSCLIAAGQVPDGAEIMTVEGLEKTEGGRILQEAYIERGAAQC
                     GFCIPGMIMSSFALLSKTRTPDDAEIREAHAGNICRCTGYEKIIEAVKLAANRWPEK"
     gene            complement(12651..14915)
                     /locus_tag="PK634_01445"
     CDS             complement(12651..14915)
                     /locus_tag="PK634_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOW96429.1"
                     /translation="MPATVQTRDPRWVGQGIPRVDAEDKVRGRAQYVDDLLVPNGWHG
                     HVVRAPVPHGLLRGLTPDPAFDWSRVVVVTAKDIPGPNCIVSHDRSMPVIASDEFLYA
                     GEPVALIAAPTLRLAREAAEHLRIDFEERPAILTLHEVADQFKHNDPALVKLCGQTIR
                     KGDAEAALARAHKVVEAEYTAGHQEQLYIEPQGLVAEPTGDGGVFIYGSMQCPYFIVH
                     ELCEALALPPEKIRVKQAAVGGAFGGKEEFPTWLAGYVALLALKAKRPVKIVYDRHQD
                     ILYTTKRHPVWSRYRTGLNRDGTIAAIAVDFLLDGGAYLTLSDVVMYRGILHAAMGYR
                     CDHVFVNGLVARTNTVPSGAFRGFGAPQAIWGLESHVDAMAAAAGMTPHEFRLKNCLV
                     QGDTTPTGQVLKWSVGSPAVLEKSLERCRFDEQWKKCSRGKPGAKSWYGIGVSFFAHG
                     AAFTGDGEARIKARAAMDLDWLAPGRPGGIVRVSSTEMGQGALTVLSQLAADGLGLDL
                     SRVSCPFPDTALVPNSGPTVASRTTMVVGSCVYGAAGKLKKALEQFAGKPIASGKEFD
                     EIASAYLNKHGRLRVEHLFVLPPGTQWDQKTFRGDSYPGYSWGCNVAEVEVDPRTLGI
                     RVKKITAFYDIGKVVNPVLAKGQIEGGLVQALGYSVMEKIGIDRKGRFDASRMQTYII
                     PTMQDIPEMDLDFVEFPFDHAPPGAKGVGELPMDGLAPAIANAIEAATGLRMRDLPIT
                     PEKLYEHSQKNLGR"
     gene            complement(14978..16267)
                     /locus_tag="PK634_01450"
     CDS             complement(14978..16267)
                     /locus_tag="PK634_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96430.1"
                     /translation="MLSLLSVAAETPADVSIRIVDEQIEDIPWNAEADLVGITCMTAL
                     APRAYEIAGRFRARGVPVVLGGMHPSLCPDEAIRHADAVLAGEAEGLWSRVVDDARAG
                     RMSGIYRADGLHSLRGLHPPPRHLLQGRRYATVHAVQATRGCPHGCDFCAISAFNRKT
                     HRQRPVDEVVAEVAAIPDKFIIFVDDNLTADRDYARRLFQALAPLRKWWITQATLAIA
                     DDPELVELAARAGCKGVFVGLETFSDRNLGAVSKSFNRVSGYREAVRLLHRHGIAVEA
                     GIVFGFDGDTPDVFAATLKQLDDLEIDVIQASIFTPLPGTPRHGMMKDRIMDGDWSHY
                     DFHHAVFRPQNMTAADLQAGHDWVTREFYRPWRMARRMWRHAFRPGGLASLPYLAAVN
                     LAYYGRTFRWKIRGWNPARGREPATAASACAPAPATV"
     gene            16538..18010
                     /locus_tag="PK634_01455"
     CDS             16538..18010
                     /locus_tag="PK634_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOW96431.1"
                     /translation="MKQSLRIPASDQPLFRLVEAAAVANPFSDDRFEVERKIAGAAGG
                     TAAPIRERAIQKVRERVAGYVRRGRADIRLYPREDAQRLRTVILFDVFHRHIQELDGL
                     IVRQQEAGDAPIEVPFAGTILQEMTDAGLTLDASRHVLAEFYQLRRAFYFIRHAALGS
                     SPCMQALRCELWTNVFTHDFNLYDRFLWDRMEDFSTLLLGETGTGKGMAARAIGCSGF
                     IPYDPVKGRFACSFTRTFISLNLSQFPPSLIESELFGHRKGAFTGAIEEHTGLLARCS
                     PHGAIFLDEIGDVDIPIQIKLLQVLQERTFSPVGSHEKKRFQGRVIAATNKPLEQLRR
                     SGRFRDDFYYRLCSDQITLPPLAQRLRENPSELGDLVGAILQRLCGKSFPELKAVVLA
                     ALERSPGPNYAWPGNVRELEQATRRILLKAGYEGDAKRADGGAEPELLEGIRAGTADA
                     ASLLSMYCKVLYERHGTYEEVARRTGLDWRTAKKHIAAVP"
     gene            18060..19031
                     /locus_tag="PK634_01460"
     CDS             18060..19031
                     /locus_tag="PK634_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96432.1"
                     /translation="MSNPSLLVLAVLLLAAGCRSPRVEQIHPMTEGEMRAVFSRAQDP
                     RRFWITVYGSEEGAYFANANRLHPDQVVALPFAAGKAQPDWPLVGVQATSKESFLALV
                     DTSSRDSWGAPDIVLGLRGTPLGPPAYDAVPEHVAEPVKGYACVIPTLRYDLMQMENV
                     LVYLRAAQGPLGYLARRDEKKTPPVLVLGMSWVNVFSFVQLNYPEKLLFLSSSSAYGP
                     SADHLIAEVPLKTVRGALAVDGLIDGEPATLVLDSAGDFELAMTEPPVEPVRVSVGDL
                     VFPRAKAVAAREHALGLPSYPRIGAKWLAKYKVTIAPKAKVVYFERP"
     gene            complement(19063..27615)
                     /locus_tag="PK634_01465"
     CDS             complement(19063..27615)
                     /locus_tag="PK634_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW96433.1"
                     /translation="MSTRSIRAATVAFSLLLLASLSAPAEVALQYFNNTWNEIADKLP
                     ELAEAGYGALWLPPPQKGSGGLSVGYDLWDPFDLGGIDQRGTVKTRYGTEAELLRLIE
                     LAHRFGMRVYFDNIMNHRAFDVPGWDEYTALDIYPGMAPEDFHLRVTEDGFYRKWDNT
                     VNWGSTWEVQNRNLSDLIDIAHEDPNANFGASEGSTYPKIHFVRQPTHPEWYDWAYTP
                     TGMAYVGFYSTNITTNMIAQYPGSFTEDVGGYLCRNVRWLIHHTKVDGLRLDAVKHVP
                     GYFFGEQWAGDKDSSSAGYCGQAQWQFNMTRGYADWDNHRDTVFDLEKSFGRNDALMF
                     GEHMGEPPPYGDYWAAGMRLLDARTHQTFNDNLGNPWGSLSGLDSADYISGKQMGQYL
                     GVYYAKSHDDNVAYREELHNAFNLTRAGLPVIYTDGNRHAEIMGASGGAFPRHANTCY
                     LGQWGDSRIPNLAYIHSHFARSWHMSKWGDGDFCAYERMDKRENGSMNDPDGTVLLMM
                     INDNYASGQSRSISTGFGHTPFVDDAYLYNYSTYGGGFYTYASQLGSVIVPPGGYFAF
                     SWRSPEQAQPWSCHGGSPVTIFQNGQEAGTISVTRRDGPDGDPGFNPYGAADANSTDY
                     SYTWSLPRVTSSTNLRFVSFVDGSAANVLMKLDGGMNLNTNNHSSGDPRDHPPGNEGS
                     TDVFEGYEQALFIHRQHREKFADTNSAHNTIGSMGSGSYTMTIGTAGLYANAGSGNND
                     WDGAETAAWVYHNPNDNDEWSAKQFFPLPQNAVNSNITVRVKVGYSSTYVNRLALYYT
                     TDGATWPEGAGGFGRGTTKVVPLTWYANGAPDGTGTPDWWSATIPAMTNGTVLRYKVG
                     AYREQGSDGFPWDVESPVDGASVSMKTTRMGVWQVTNINLRTVAYRPHNDWGTVSTGL
                     VDGFHVMRSRAFLDRGGKASIFNTFTQPFYLDMDRPGGEVKYPAESETLGSKEYGAVV
                     RTDPTVTEVWFNISDSDKTNDDGSTGQTNGIGTNAVGQLSWARAYKVTPSLYISSAYP
                     DEWRFTYRNIPSGNSNATITVKLRELSSSTNLTLSDTLGHFTTLTRNVKTDAPAYDLY
                     VAWPQYDGDLVFSGYVAKVWFSQSLADGIDNDTLKSRFIFKINDVAQGRDDWYFEWDV
                     SGSHHALAIPLSDMYNGDTNYLHNLQVLHTNAAGLGITLQASRIVRAQSSADSILIQI
                     TDPPEYDSDGAPYEIVLPDVASPSPTQRQYRVQVETDTKARNVWIEFTNSVGFAPRIA
                     STSNQLTGTVSVWAGSNTVAGSGTLFHQELGVGNTIRISTNYLVVSQVVASNQLILTA
                     TYPGATASGQTAWRVDPNPSVSGNKQIWGFLWTNMTAGHFQFYAHADTNSASGTTKHA
                     SALRNATVVLMQSVNSNTNDLDDDDDGLQDDPEVTSTNLPDTNPETWNNGQVHIWYVY
                     GRTDPLSPDTDNDGLPDGLESGWGGPSSGTDTNTDTNGDGWKNFIADQDIPIYNTTDN
                     SGYSRYNFNRSRTDQICGTTTDPSKPDTDYDGLLDGQEDLNRNGRVEIGTLNGSGVAT
                     GVLAHPNVPTVYNSSQVDSDSLAANARFLEPDPNNGDTDGDDLADGAEDADLNERVNL
                     ALLWPAGTTTTFVVTWTNNSTNILGNGLSGVRSRALDYSKLWTDHPRPRYTNGVWQNT
                     NVWPRLLVLETDPLDRDTEDDGLPDGWEQRYGLDPFNDGWYSIQTGQMSPTNTRQGAD
                     GDLTSCGVSNYQHYLGGTDPRVCVTSPPPPEGSIVIGRGEALGVINGTTNYQEFTEWT
                     WSDLRALDYYEGGGNNNQQGDIYKAWDDWDTSRDLLAFYARDGGSSDGKLYFRFDFYD
                     LKALAEQGNLDLYVVIDTGNQSIGERKLPDDVDTLTEMRWEAVVALYDASAGTVYVDT
                     NPGQNTSTFAEDLFTYGGVQAQPQHFGSAYYNHELDAVEFSISRTALTAIGWNGSMAS
                     LNFQVYSTRDGTQNDPVGAGDLGGRSDIRDAIWNDYIAEDYWEAQAGLSGANSVLYNW
                     IPGTVRAGRAKAAIVLHGNQHDQPGHVAQLLVNTGAGAGYHRPLLAHEVFGQALNLHV
                     TPTLAASLQWARVDTNASPTWRDGPTLNETVRRLVATNVVWMLGSTFSDHILPYFTKE
                     FNRDNIRLASEYLSEIYGASFTTNSVFWTPERVLDDDTLAKITNAGFRATVLDQNTHL
                     YYWFGRTDSMGDNGYSINRIGNVDCFVINNLPTEYRFSNTDNGLSLSMRALFNRRARG
                     DQQRVTTIFSNWEDFSDNDDADAYDRNLRWIANRPWIKLVGLEEVLSGAIDTDGDDVG
                     NAWWKNDRGSPSLSKQSHDWINHATRGDYDNWYVGEATKREGLQNKVFEIRPGTNVSQ
                     AYGMLYFGGAVSSAWRQVESIADTNIARLARAVIHASVFETAFHNEDNNDLRRWSTGE
                     YLYPSESYLTLIDFAKTAQAQTRLAALYEEVDDWAAIASGVTTPQAAQADVDLDGENE
                     YVLYNDRLYAIFERIGGRMTAAFARDPSDGAVRQVLGNPVSYAGVETEVEGTGHVATN
                     GTTLSYRTSGLKDWWAGTTNYVNDLYTFTYWTNGWRMTSADGKIRKIITLNQGTNLFQ
                     VSYNVTNTLNGGVLYVRHGFSPNLDNLLRQGQNTLGVEEHSGGVMTLANLGGGKTVQA
                     RLGYGDAGHNAAFNLQAVDDMPGTNAVFTITMRNQAQTHQVEVFGTNAFAFSMGFSVL
                     GTGDTDSDGDGIPDQWELDNFGNLTTADEDSDWDDDTLLDWQEQVADTDPKDKDNFFE
                     IASTARNSSGFIVRFGTANGRKYYIYYNNTGLLNQSWSNATPTGIDGTGALYEWTDNG
                     TQTSPHPNLMTNRFYKISVAEP"
     gene            complement(27811..28467)
                     /locus_tag="PK634_01470"
     CDS             complement(27811..28467)
                     /locus_tag="PK634_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOW96434.1"
                     /translation="MLRASARAKSIPIVLLAGVAAVLFAPLFRWGLAKPALFWWWMSG
                     LLLLLNTSMLVSRPDLRRQLVSDFRSDAFRKLVIGIVSAAGLYLIFLAGRMLIRGWLP
                     DSEQHISSVYLLRPALSAWLIALLMITIIGPGEEIFWRLFLQPRFCARFGDRTGLLIG
                     ALLYAGVHAGSGNLVLILAALVAGLFWGWLYATTSSASIIIYSHALWDVSVFVIAPFS
                     "
     gene            complement(28467..29405)
                     /locus_tag="PK634_01475"
     CDS             complement(28467..29405)
                     /locus_tag="PK634_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOW96435.1"
                     /translation="MPPEMHPARKWLVAARTFALPASIMPVVFGTTAAATQGGAAFHP
                     GLFAAALLAMVLLHTGANMLSDVTDFRKGLDREATPASGSIVRGLLSDAQVRRGAILL
                     LVAGSAIGLGLALRIGWPLFWIGVIGVAIGIGYTLGPALKYRALGDLAVFLDFGILGA
                     LGAWTVQTGQPSWRPALWAVPMSLLVIGILHANNWRDIATDRERAGIRTVASLLGDRG
                     SLLYYGALVFGPFIIVTALAAVGIARPDAACGMPWPALLVWLALPLALRLWDKARKRK
                     APAHPTDFVALDGATAQLNLAFGLLCTAGFILDRLL"
     gene            29613..30788
                     /locus_tag="PK634_01480"
     CDS             29613..30788
                     /locus_tag="PK634_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96436.1"
                     /translation="MKRAPMARARCKKNARRISLVYDEAMQDPQQFHMDVSRLPAERE
                     WRGARSLGIFLMLFSLVWGGLPAAMLVLTLAREGFSPAIMPALVFPVVGAAMFLVGLN
                     QIVLRGRVRIEASAVFFERRSLFKRSEWTEPLARYAGLLYRTEHHSGGKNRPSYTLYI
                     VELHHPEKARRVTICQSKSPDGMRAAWENHCRALNMAALEESGGQLIRRGPADLDKSV
                     RDLARERKLDLAFDPRQAPPQGIQLEIGRDELRLAIGVPALPLKVAVPWALAALAMIG
                     LPFVVRAAPLFVATLGLISLAVPVAWRVLSPRFRQVLVVSRAEVRVGWETPWGPPAET
                     VLPAGEIEQVEVRRGAGGQSPTLHIITDRREVSVGPFLKPADRDWLRHCILAVISAG"
     gene            complement(30797..32929)
                     /locus_tag="PK634_01485"
     CDS             complement(30797..32929)
                     /locus_tag="PK634_01485"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOW96437.1"
                     /translation="MKGWKRWLGPCALMVGGMVWAAARAPASAPEAEASEAARLVARR
                     LPQWHLTQLPMDDDRAVAALDRFLAFLDFDRSYFLASDIQRFQLAATNLDNRLIAGDV
                     SFAQEAYDTLRTRVSNRVDFVEGLLKQGFELDRDESYTWKRKDAEWPADEKAWDDLWR
                     RKIKNQYIAHMVNRRMADAARTNEPAASLPEEPAVEPSEEVDLSTDLRLTPEEAIRKS
                     YRQYLDVLNDNRAEWPVDRYLTAFTQAYDPHTDYMSASQTEDFEIGMQLSLVGIGARL
                     TSEDGAAKIDQLIPGGPAEKDGRLKAGDKIIAVAQGDGEPVSILHWPLSKAVRLIRGE
                     KNTKVVLSVIPASDPTGTTVVKIDLIREEVKLEDQAAKSAVKEVVGADGRTRKLGIIT
                     LPDFYADVRPLRGLGAEARSSAQDVRRLLRELSTNGVEGLVLDLRNNGGGLLAEAVRM
                     TGLFIERGPVVQVSDGRRSQVMNDSDPEVVYDGPMIVLVNRQTASASEILAGALQDYG
                     RAVIAGDSKTHGKGTVQTLADLKDGQPALGKLKVTTASFYRINGGSTQLRGVAPDIVT
                     PSSLDTLEIGEETLPQALPWSQIRSLTYALATPLREQMPALRSKVEARRKSDARYQAY
                     LELLGRLAERQKDPTVTLNFEKRLELARTEKELSDQLREKDEGGDQQADVMLNEALQI
                     LSDLISMNAEPAQPADAEHPVTAAAPAG"
     gene            33106..34071
                     /locus_tag="PK634_01490"
     CDS             33106..34071
                     /locus_tag="PK634_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96438.1"
                     /translation="MNRFELRRAAGVAGLLVLGAGFVFLLFIGSSLGWFEREPSAPAR
                     ARPGGTPAALSAAPAAAAPAAPIAAPSRRAGPSGGGPGALKDAEALETRLREIETENR
                     RLRGEVQDLHGRLVGVLNWVLTNFRGTYPLPEPLMARLQVPPVTEDYTLHPEAAALIK
                     VTPEEEQKINDVFAYARSYLAEIEAAMLTVTSPRPDKVILHIPPFAEDGAALREDMYA
                     ALEITLGADRFDRFLKVSEAGLKSSFYRFGEASRTLVFELVYAGEGEPPQLKIKDGWI
                     METVPGAKEITATEATVTNLPSRYAAYSPWLPDYLVQGFATEAVR"
     gene            complement(34052..34243)
                     /locus_tag="PK634_01495"
     CDS             complement(34052..34243)
                     /locus_tag="PK634_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96439.1"
                     /translation="MSRHEQKTAKESAELMYVKCAACGKWIDVKPGEMNKVSHGICPA
                     CYQKEMAKWKGPPPTEQPR"
     gene            34391..35188
                     /locus_tag="PK634_01500"
     CDS             34391..35188
                     /locus_tag="PK634_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOW96440.1"
                     /translation="MRRNIIDVHTHVFPDDLAARAIQHLSGNSGERAFTDGTAGGLQA
                     SMRRHGIALSVTQPVSTRPAQTPSINTYVMELKQPGLIHFGTLHPDYGNPDAEIERLA
                     AGGIKGIKFHPDYQNFFVDEERMFPVYGRMARAGLIAFFHAGVDIGLPPPVHCPPDRL
                     ARVLERVPDLPVVAAHFGGFKMWDEVARHLVGKPLWLESSFTLSWLPAADFTRMARRH
                     GIERVMFGTDSPWADQGAEIALMEKTGLNEAELDAVFHGSAARLLGL"
     gene            complement(35222..39166)
                     /locus_tag="PK634_01505"
     CDS             complement(35222..39166)
                     /locus_tag="PK634_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOW96441.1"
                     /translation="MPTDDERPAGPVHRVIRFCLEQKLVVGLLVAFIAIWGVIVAPFD
                     WAHTGLVRHPVPVDAIPDIGENQQIVFTDWMGRSPKDVDDQVTYPLTVSLLGMPGVRT
                     VRSYSMFGFSMVYVIFEEGVPFEAAQNRVIARLNGLPEGTLPAGVKPALGPYATALGQ
                     VFWYTLEGRDPDGNPAGGWDLDELRSIQDWTVRYHLQAAGGVTEVAGIGGFLREYQVD
                     VDPDALRIHNVSLEEVFRAVRASNLDVGARQIELNRVEYLIRARGFLRGIKDLEDTVV
                     KVNGNVPIYVRNVAKVALGPAFRDGALDKEGAEAVGGVVVVRHGHNPLDAIRNVKARI
                     AEIAPTLPVKAVADYRRATKADLEQWARGEGFEAFRDGALDQEAWLARLNAVPRERWP
                     AGITTSRVTVVPFYDRTGLIYETLGTLNAALYEQILITTIVVIVMAAQLKLSLLVSAV
                     MPLAVAFSFIAMRLFGVDANLVSLSGIAIAIGTIVDMGIITCQNILQHLADARRDESR
                     LEVIYRAAGEVGGAILTAISTTVVGFLPVFAMSGPEGKLFRPLAFTKTFCLVGSVVIA
                     LTIVPAAAHLLLGRRPPSNRIQQALGAGLVLAGVAMGVLFGWWRAAPLFALWGGYVAV
                     EERLPERLRRVAPGLANALAVLLVGALLAGHWLPLGPEKGWLRNFVFVAVVIGALQGF
                     YYLVERRYARILRWCLEHKLAFLSLPAAIVIAGYTAWLGFDRLFGFVPAGLEKAWPAA
                     RALRQSGPWSWAVHAFPGFGKEFMPPLDEGSFLYMPTTMPHASLGEAMDIVRKQDMAL
                     RAIPEVESVVGKIGRAASALDPAPVNMIETVIAYKSEYATDQAGRALLYRYDRQQRQF
                     ARDADGRLVPDPRGKPYRQWRDHIRTPDDIWDEIVRAAEVPGSTSAPRLQPIAARLVM
                     LQSGLRAPMGVKVSGPSLEAIERAGLDIERFLKEAPGVEPSAVIADRIVGKPYLEIEL
                     RREALARYGMKIEDVQMAIEVAVGGVRLTTTVEGRDRYPVRARYLRERRDSPEALQNI
                     LIPAMDGAPIPLAQLADIRYARGPEAVKSEDGFLVSYVLFDRKPGFAEVDVVEACQAY
                     LADRLASGDLALPPGVSYRFAGNYENQLRAQKTLSLILPLTLFVIFLIIYFQFRSVVT
                     TGLVFSTIFVCWGSGLLLIWLYGQPWFLDFSVFGLNLRDLFQVRPVNMSIAIWVGFLA
                     LFGIASDDAVVMCAYLKQRFEEVRPATREAIREATVFAGGRRIRACLMTTATTVLALL
                     PVITSRGRGSDLMVPMSLASFGGMLLEVVTMFMAPVLYSWVREKSLNKAARP"
     gene            complement(39156..40595)
                     /locus_tag="PK634_01510"
     CDS             complement(39156..40595)
                     /locus_tag="PK634_01510"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW96442.1"
                     /translation="MKKRYLAAGILLLAFGFFAGRAGPRRGAPGSAAAGGAETAAIWT
                     CSMHPQIRQPGPGLCPLCGMDLVPAAASSRGGDEPPRRLALSPAARRLAEVDVTPAER
                     RPVAVDVRLAGRIEPDETRVATIAPRVGGRIDRLYANFTGIAVKAGDPLADLYSPELL
                     AAQQELLQAGRAGALLDAARERLRLWGLTSEQVAEIERGGQVREHLAFHAPLGGVVVE
                     KDAREGQYVEAGMPLFTVADLSTVWAVLDAYESDLAWLREGQETALEAEAYPGEPLRG
                     TIAFIEPVVDPMTRTVRVRADVPNPGGRLKPGMFIRATVKASTAGDGTGAAPLVIPAS
                     APLITGARAVVYVAVPGEEGAFEGREIALGPRAGDFYVVRSGLEEGERVVTSGAFKLD
                     SSLQIQGRPSMMSPPAALAAESKAQSVCPVMGGKIDKKFYTDYQGLRIYFCCGACDAA
                     FSAGPEKYLEAMRAAGVEPEPLPGGPHAH"
     gene            complement(40592..41917)
                     /locus_tag="PK634_01515"
     CDS             complement(40592..41917)
                     /locus_tag="PK634_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW96443.1"
                     /translation="MTPRRPFGIAGALALALLALEGPAARGGERPDEQATLDAILQRA
                     FRDNPGLKAMRQKWAAADERVPQAKALDDPQLSYEYFLEQKDVRWQAALTQMIPLFGR
                     RGWMAREASAEARAARHEYEAARLDLYDRVSRAFYDYHLLGRSLAVMEEQYNLLADLE
                     NVIRSRYEAGSAGFPDLTKAQVEKDRTASELASMRDERGVLSDRLSALLDRPAGPPLP
                     WPAVEPSASALLDEPALLAMIEDLNPELKAAQAMTDRARATQELARRRGRPDLMLGAG
                     VMSMPSMEGGGSEYDASVMVGITLPWWRGKNSAGRREAAAMAESARNEQADRFNELKT
                     ELRMAVFKVRDSERRIALFRDSLIPRTEEAYAVARQAFAAGRAEFMDVNDVQRMLLEF
                     RLAHERALVERELALVEIGCCIGKYGADLESVLAAPPRAGPGPAGGGAR"
     gene            complement(41921..42202)
                     /locus_tag="PK634_01520"
     CDS             complement(41921..42202)
                     /locus_tag="PK634_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOW96444.1"
                     /translation="MKNHKTRHDENRGRLARIEGQVRGIRRMIEEGEYCMDIVTQVQA
                     SVAALQAVGRRVLKKHMEHCVADAFRSRSKRDIETKIAEILKVVRQCGG"
     gene            42385..42822
                     /locus_tag="PK634_01525"
     CDS             42385..42822
                     /locus_tag="PK634_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOW96445.1"
                     /translation="MTRNILVQWAQIQRKLTGLSDHTLEPHGAGTWSPNTDVYESADD
                     VVIKMELAGVPAESVHIVLENRVVRVEGLRRDPQSGESAAGYRFRQMEIEYGVFSRTI
                     ELPFAVDGGQARARMQNGVLSVRLPRAREADGHRITVTTENQP"
     gene            42819..45233
                     /gene="lon"
                     /locus_tag="PK634_01530"
     CDS             42819..45233
                     /gene="lon"
                     /locus_tag="PK634_01530"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOW96446.1"
                     /translation="MSDAPESSSPPPGPADPPPANGERPSPSPIPPVLPVLPMSDLVL
                     FPSMVAPLVVNTPKSARLIDAVTAGPRLLITVLQKSRAASDDAVGFADLHEYGCVARL
                     IKMLKFPDETVRILVQGISRCRLARPHGNPNFLEVYHAILKDEVEDSVETMALARNAS
                     QKFQEIITLSPTLPEELKIAVFNVDGPGALTDLIASHLNMSLEDRQALLEEYRVKGRL
                     NRLTTLLNREFEVLRMGTEIQHKVSENFAKTQREFFLREQMKAIRQELGEKDQQQMEG
                     QEIEKKIAAARLPPEALEAANKERERLLTMPSASPEYAIIRTYLDWLTELPWAVATED
                     ALDIARARAILDRDHYDLAKVKDRILEYLSVLKLKKDLKGPILCFVGPPGVGKTSLGQ
                     SIARALGRKFVRMSLGGMRDEAEIRGHRRTYVGALPGRIIQGLRKAGSRNPVFMLDEI
                     DKLGQDFRGDPSAALLEVLDPEQNTAFTDHYLNVPFDLSSVLFITTANLVDPLPPALH
                     DRMETLELPGYTANEKVHIAGRYLVPKQIAAHGLKRSQLAFPRATLQRIIGDYTREAG
                     VRNLERQIASICRKTARRVAEGRRGAKRVTPEALHGLLGPKLFEAEVAERQLEPGIAT
                     GLAWTWAGGDILFIEATRMPGRGLLTLTGSLGEVMKESAQAALSYVRANARKLGLPAD
                     VLKSTDIHIHVPSGAIPKDGPSAGIAMLSALVSLLSSRPVRAGVAMTGEITLRGKVMP
                     VGGIKEKVLAAARAGIRKIILPARNRRSLEDVPSEVRRRVKFVLVDNADSALRAVLGP
                     GRKAGT"
     gene            45230..46027
                     /locus_tag="PK634_01535"
     CDS             45230..46027
                     /locus_tag="PK634_01535"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOW96447.1"
                     /translation="MNRGVVSAVLVVWAGAFSWAAMDEEPLLRPDASWPDAVELVQGV
                     VAGLPDVPVRMEAQLQSKDRRGTIEGTLNAEMKLDWYGQPPSAEYRIRDAFGAEREAL
                     RLDWSEPGRPVYRYSRGEPPASEPLPDLYRPIEGTDISWIDLSLSFLWWPGGRTVGME
                     KIKGRMCYIVEMPAPAGEAGGYAGVRLWIEPRIRMMLQAAAFDAGGEVLRLVEVKSFK
                     KVRDLWLIQNLDVQTFPGRHRTSLRVRHVAVAGEAIEEGGKGGGEDS"
     gene            46038..46862
                     /locus_tag="PK634_01540"
     CDS             46038..46862
                     /locus_tag="PK634_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96448.1"
                     /translation="MADIQLKCAQCGKEITVSEFVSTEALTCPACGAALSMPERKAEA
                     VPLKIRLQERESRTALTGHEMDVDVLARAVAAKETDSVMGEVHKARTQVKKSTGIWLW
                     IILLLVGGGLVGWQHAVAEGIGPAGLADSYGMARAGIAGLAAVLVLAVAFYESWVQGV
                     LCLLLPFYILYYTIVRMEFNTVRGLFLGVCVAIAVEVRLLPDQSLLLAAQGRFDQFVT
                     AVNQKIKRAGEPPSMPPSRRRGRHAKPKSFNAVPVFFDVGTCSRRQGDSRELLLPA"
     gene            complement(47232..48203)
                     /locus_tag="PK634_01545"
     CDS             complement(47232..48203)
                     /locus_tag="PK634_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOW96449.1"
                     /translation="MKDATSIRHAIGVDFGGTFIKMALVSERGEIKARAKIPTKDAPG
                     LDGWLDAVGRGLEQLKDKKAPADAPLAGIGVGVPGFVDFERGFIYDLANVPGWTNVHL
                     APRIEERFHLHVRVDNDVNVMALGECTYGAGRAYQHAVFVTLGTGVGGGIVINNQLYR
                     GAYSMAGEIGHVPIRMDGVKSPQGRGGLEQYVGNRRLVERVVQALQQGRASLIRELAQ
                     GDLSAITVELIAKAAGQGDALALELFDFMADCLAAAFASVAYLIQPQAFIIGGGIAQS
                     GAILFDPLRRHLTERLSPVFAERIVVKPAELGNDAGVIGAATLSMME"
     gene            complement(48200..48985)
                     /locus_tag="PK634_01550"
     CDS             complement(48200..48985)
                     /locus_tag="PK634_01550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOW96450.1"
                     /translation="MFFDTHAHFDAFADPAEARAALDRAEAARVGRVLAVGGSPDTNR
                     AALEALAEWPGRVFAALGFDRDQASAAPSLPRLEADLARPGVVALGEIGLDYHYEPET
                     AAAQKTLLAAQLEMARRRRLPVIVHSREADADTLALLGDHARAWPGDPERVGVLHCFT
                     GSAEFAARLLDLGLMISFSGILTFRNADPLREVARGIPGDRLLIETDTPFLAPVPHRG
                     KRNEPAWVVPVAEVLAKIRNDSLELIEHSTTANAERLFLGARS"
     gene            complement(48985..50604)
                     /locus_tag="PK634_01555"
     CDS             complement(48985..50604)
                     /locus_tag="PK634_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOW96451.1"
                     /translation="MRTKGPGVLESRDARLFSLALALLACAARADDPALRTVVLANRR
                     VPESVELARHYMQARGIPADRLCALDLPAGETLMREVYEVRLRDPLLAFLRERRLIEQ
                     VRRPSDSVRRHESEWQTLSTSIRYVVSMYGVPVRIADTRWRIGEAIANRLRRFDLKDA
                     AAVDSELALLLNADYPIRGPYPNPLFNQMNEADAPPSAALILIAARLDGPDAATVRRM
                     IDDARTAEARGLEGRAYVDGRGLAHGSYALGDFWLSEAAERLLREGLDVVQDSAEPVW
                     GPAFPMEQAAVYLGWYNENATGPFLQPGFRFEPGAVAYHIHSTSAEKLRTPAEHWTGP
                     LLAAGASASMGCVGEPLLNLTPHLSVFIDRLCRGLTFGESAYLAQPVLSWQVTVVGDP
                     LYRPFRHVPGEESAPATDWARARAVNQLVRAGRFNIGLGLCRRWLAETGSPILREKLA
                     ELYARNDLFPEAAEEYRAVAEQAATADTAVRAGALALRILRVLNRTTEADALEQTLRG
                     RWKDSVVLPWLETGRLEEDAPAESPSLTPGS"
     gene            complement(50614..52740)
                     /locus_tag="PK634_01560"
     CDS             complement(50614..52740)
                     /locus_tag="PK634_01560"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOW96452.1"
                     /translation="MDMTLLYMSLGAGVLALVFAFVRTTWINKQDPGTPRMQEIGAAV
                     REGAMAFLKREYKVLAVFVVVVAALLAFLNIKVTGNKSLGLVALSFVVGAFCSVLSGF
                     FGMRVATAANMRTTNAARQGLPQALAVAFAGGSVMGMCVVGLGLLGLSALTLAYLKVF
                     GSETAALTNTVLPIITGFSMGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPAVIADNVGDNVGDVAGMGADLFESYVGAIVSAMVLAAVATVPAGIAGSAMNLVL
                     LPLVIAGVGIVFSITGTFMVKTKEGGNPQKALNAGSLAAAAATAVASYFVIAKLAPAQ
                     FSLTIIPGMEPKVYQSIGIFWATIAGLAAGVAIGFLSEYYTAEGKGPSNRIADSSTTG
                     AATNIIAGLGNGMLSTAFPTILLGAAILVAYKTAGLYGIAIAGLGMLATTGIQLAVDA
                     YGPIADNAGGLAEMAHLEKEVRQRTDKLDACGNTTAAIGKGFAIGSAALTALALFAAF
                     GQTVGLTKIDVMDPNVTAGLLLGAMMPFLFSAFAMNAVGQAAFAMVEEVRRQFKSIPG
                     LLEGKEGVRADYARCVDISTAAAIKKMTVPALLGVLTPVVIGFVFGTEMLGGLLAGVT
                     VTGVMMALFMANAGGAWDNAKKHIEAGALGGKGSPAHKAAVVGDTVGDPFKDTAGPSL
                     NILMKLMSVVALVIAPILKAVSPLLK"
     gene            52914..53699
                     /locus_tag="PK634_01565"
     CDS             52914..53699
                     /locus_tag="PK634_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOW96453.1"
                     /translation="MLKLKLDRPLVVFDIESTGTNKKADRLIDLAVVKFWPDGRAESF
                     AFRVNPERPIPPESTEIHGITDEMVKDCPTFQQIAPRVMDVFRGCDLAGYNILGFDIP
                     LLTEEFTRAGLVFDTQACRVLDAQRIFHKKVPRDLPAALAYYCGEMHLDAHDAMADVQ
                     ATVRVLEGQFERYADLPRDMERLDEFCNPRHPDWVDRSGKFKWVGREAVINFGKKQGT
                     PLAEIARTDPGFLKWMLKSDFAPDALEIAKNALEGKLPRPPGG"
     gene            complement(53703..53924)
                     /locus_tag="PK634_01570"
     CDS             complement(53703..53924)
                     /locus_tag="PK634_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOW96454.1"
                     /translation="MEEQVKAYIEQIRPYIQSHGGDVEFVALRDKVVQLKLLGACRGC
                     PGALMTLHQGIETQLREVVNPELSVERVD"
     gene            complement(54094..54744)
                     /locus_tag="PK634_01575"
     CDS             complement(54094..54744)
                     /locus_tag="PK634_01575"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOW96455.1"
                     /translation="MNRPLSILPLLAAATLAAAGEPAPTAPAWKDGIATLGSVRVDPT
                     TRTVLATGWVNQVSGAIELLACGKGGKTHESVFVLDLNPLDLQTGLLLLGLKPGTPPE
                     GLGQPGAAGPALDIWVDWELKGKRRSLPAERFIRNVERNRPLKQIPWLFTGSMFEDGE
                     FKALAEESLVVTYWDPWAIVNIGDPAGSNDEILVVNDKLVPPLNTPVTLRFRPRQR"
     gene            complement(54741..55439)
                     /locus_tag="PK634_01580"
     CDS             complement(54741..55439)
                     /locus_tag="PK634_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96456.1"
                     /translation="MHVLRQSVKGIVGLWLLAGFFAGPADADLMIFAFASWAKAAEPA
                     APGEPAAETPAQTRETLREISRRLDQAYGNMNRGRYQDALAQFRSVLKYDPKNHRARF
                     GLGNIMIQLKQYQEGRDIFEALIVDNPDDYSLKNNLAWLYATAEDNSIRNGARALELA
                     QDSLLMAPKDYHVWSTLSEAYYILGQYPRAQRAAQIALDLAAAEKGTERNIEEYRRQM
                     EKCRRAAEAMSIIE"
     gene            complement(55439..56401)
                     /locus_tag="PK634_01585"
     CDS             complement(55439..56401)
                     /locus_tag="PK634_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOW96457.1"
                     /translation="MPSWASRLAELLRRNWGGKLAALVLATVTWYGIQSVINFEARIR
                     EVPVTLRLPEGWAALDQSVSAVDIVFRGSEEDIRFLNRDQIKVEIDLNPRAIKGTNLV
                     ARFKILPRHISAPNTVRPVQVRPDEITVSLDRQEQKAVPVKADFQGEPAEEFEVERWE
                     CAPAAVTLSGPHSRLQRIESVNTIALDLEGRTKSFRKTHVPVQAPPETVVVEPVPAQV
                     SVEVSLAERTSTETWKDVPVCVLSRPGAREELSVVPVSATLTVRGRAEILKKLDARDV
                     RVYADGSDVEKAGTYELPLRIHPPPGLAVVKTEPPVVSVTWGEP"
     gene            complement(56394..57260)
                     /gene="cdaA"
                     /locus_tag="PK634_01590"
     CDS             complement(56394..57260)
                     /gene="cdaA"
                     /locus_tag="PK634_01590"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOW96458.1"
                     /translation="MNWFHQIQWPGLTGLLEIALFAFAFFYVIMFFQGTRGVQILFGL
                     AVVLGVMIVLTKVLRLDTVNWLVARASLYLAVALVIIFQPEIRRALAELGKPHVFASA
                     AAERTVVEHIVQAALMLSERKIGALVAVEREIGTRAVHETGVKIDSVVTPELLASIFY
                     PHTPLHDGGVLIRGNRLVAAGCMFPLSAREEFSKALGTRHRAAVGLTEETDALVVVIS
                     EETGTISVAYKGRLSRGLDEERLRRMLSAVLLKSPRPQSRMGRVKEQLDLTPEGVAKT
                     DAARQEEARPNA"
     gene            complement(57257..58117)
                     /gene="folP"
                     /locus_tag="PK634_01595"
     CDS             complement(57257..58117)
                     /gene="folP"
                     /locus_tag="PK634_01595"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOW96459.1"
                     /translation="MNDHPTLLWTARRRRLELGRRTLVMGVLNVTPDSFSDGGRFLDP
                     ARALEQARRMIAGGADLLDVGGESTRPGAAPVPGDEEIRRVLPVIEAIRAESDIPISV
                     DTAKAAVAARALEAGADVINDVTALAADPAMPAVAARYGAGVILMHMQGTPRTMQADP
                     RYADVVREVRDYLAGRIAALAAQGLAPETLAVDPGIGFGKTLEHNVALLHDLDTLAGL
                     GRPVVVGLSRKSFLGRITGREAGERLAGGLGAAAWAIARGAHVIRAHDVKETCDMVRV
                     VDMLRKESAT"
     gene            complement(58154..60082)
                     /gene="ftsH"
                     /locus_tag="PK634_01600"
     CDS             complement(58154..60082)
                     /gene="ftsH"
                     /locus_tag="PK634_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOW96460.1"
                     /translation="MEPPIYSPKETGPGKTNVPLRGVALWLLLLALFLTVYQMFSQQQ
                     DRYERIPYSPDFIELVKQQRIRKAEVIVEVSGLHYVRGELLDKDNRTKRPRRFRTEIL
                     VGDDLIRFLRESGVPFEFKTQNPYIWQLVSSVAPFLLVFGIIYFFFARQMRMAGRGAM
                     SFGKSRARLMSRERNKVTFADVAGIDEAKEEVQEIIEFLKDPKRFQKLGGRIPKGVIL
                     VGPPGTGKTLLAKAIAGEAQVPFFSISGSDFVEMFVGVGASRVRDMFEQGKKNAPCII
                     FVDEIDAVGRSRFSGIGGGHDEREQTLNALLVEMDGFDTQEGVIIIAATNRPDVLDPA
                     LLRPGRFDRQIIIDLPGLEGREAILRIHSRRVKLAAGTDLRRIARGTPGFSGADLSNL
                     INEAALLAARQNLDAITERELEEARDKVRWGRERRSRVLDEKEKKLTAYHEAGHAVLL
                     QLLEECEPLHKVTIIPRGAALGATMQLPEKDRYTESRSKLLATLTGLMGGRTAEELVF
                     GDITTGAHNDLKQATHIARMMVTEWGMSEALGPLNFGEREELLFLGREVSRTNEVSEE
                     TARKIDEEVSRIVREAHDKALNLLRGHRESLDRVAQLLLERETLDGREVAEIIEHGRI
                     LSEAERAAPGASPPSPPA"
     gene            complement(60209..>62767)
                     /locus_tag="PK634_01605"
     CDS             complement(60209..>62767)
                     /locus_tag="PK634_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96461.1"
                     /translation="GRRDHAIVVSPKKALGMTLYEVNPLTVKATSNDKAGRSTFDDLL
                     DGNLDSFTNFNLGYLNKIQVNCLWFQPIHPSAVTTKGDPSGYDPGSPYATRDYFSVAS
                     VLGDADTEADALAEFTNFVHRCDTNASSVGTINVMLDGVFNHTAWDAVMGQGGVDLGY
                     ATSATQTMGRTRPAWYAYWQDYGAPATFYNSEYDNDIATAPDRGDFGKWDDVAELYFG
                     KYSALVRHNPDNNGDYNNEGDTYDYAGMDTNTMALWRYFGYYTEYWLKKTGHPTNNNN
                     AAYDDYGIDSLRCDFGQGLPPQLWEYIINRTRKMKWNFVFMAETLDGGSPGYRSNRHF
                     DILNENLVFQFTQAHINYASSLRQALEDRRNSYSSGEVLLNLTGHDEVMADNDPWVVA
                     SRYGSVSMVDGLPMIFYGQEWGIEQFNASDPDNKNDGFLNWHELNFGKYIPHFKQWNK
                     LTVWQQPPDYSEGLAQWYGRVNWARLNSPALRSHNRYFLNKVGGGEEGTIMAAAKYQT
                     AYASPQTSDVVLAFSIFFRHGEAHFSTAASFDLTGCWSLLGLDTGKTYRVRNLASSDA
                     SAYLTSGWPKTGADLYNNGIYVALGGGTVNPITADGEIVQYLKIEEYTATNHAPAINL
                     PGPHTLPVGSTTSFVVSATDSDGNAVTVTNVTKPSGATYIGTNFSWTALPTAFANTTN
                     ALVFVANDGQGATNSIVTNSTVIVVPYDSDSDTLPDGWEWDHFTTLTNGSGGDRDSDG
                     APNGDEYVAGTQPTNVSSVFEVQSVIVPAGLTNYQVRIPTQPQRHYTIYFRNSLTNGT
                     AWSGFGNTNLGVGTWLETGTVATTFTFTDDQTANTSSNPPPDNRRFYRVRVRQP"
BASE COUNT         9475 a        20951 c        21900 g        10441 t
ORIGIN      
        1 caactcgtcg ttcttggacg cggttccggc gcggatctcg gcctgccggg cgcggtatcg
       61 ctccagctcg gcgtggatcg cgttcttccg ttcggcgccg gaggaggtct gctcttgcag
      121 cgactgccat tcccacgcga ccgtgtccgc gcgctgccgg gcctggtcgg cctcctgggc
      181 gatgatatgc tgctcgccct cgcgctgggc cagggcgtga cggcgctcgt cgagctggcg
      241 gcgcaggccg gtctcctcgg cctccgccgc cgcgatatcg tcccgcagcc gcgccgcctg
      301 cagttcaccc tcggtgtgct gccgccgcag gtccgccagc tcccgcgtcc aatcctcgag
      361 gtactgccgc cgcgccaggg gcgtgatctc gtgggcgccc ggcatccaga attcgagcag
      421 gccgtttccg cgccgcaccg cgccgccggg cgtcaccagc gtcgcgcccg ccgggatggc
      481 ggcgcccggc gcgggcagaa cgtccacgac gcgcacgccg cggagcaggc gatcgacggc
      541 cgggcgccac tcgggggcgc agcggacgcg ctcggccagc ggccggcccg gcccgtccgg
      601 ccaggggtcc ggtcccggcg cggcggcggc gaggggcagc agacgcgcgg cgccggcggc
      661 ccgccgctcg agttggctca gcaggtccgc ggcggtcgac tcgtcggcga ccgccagggc
      721 gtccaggccg gtgcgcaggg ccgcctccag cgcgacgcgg taggccgggt cgggctgcag
      781 gtgatccgtc agcgcgccca gcacggcgcc cgcgcgaaga ttccagtccg ccggcgggtc
      841 gagcagggcg cgcgccccgc tcggaagtcc gtcgagccgc gcctcgggtt gatcgagcat
      901 ttccacctgg gcctcgcgcg cggcgagccg cgccttgagg tcggccaggc tcttcaaggt
      961 gtcggccagt tggcgggcgg cgctgtcttt cgccgccgcg cgctctccgt ggcggccctg
     1021 gagcgagccg acttcgtcgc gcaacgcgcc gagggacccg ttcatctcct cctggcgggc
     1081 cgcgtaaatg tccacggcgt ggcgcagctc cgactgctcg gcggcgagcc gctcccggcg
     1141 cagggtcgcc gtgcgctcgc tggcctcgag gtcgtagagc tcgttctgca ggcgcgaggt
     1201 ccggctctcg atgtcgatca tctccgtgct caagtcgtgc aggaactggc gcgtcgcgga
     1261 gagtttgtcc tcgtgcgccg ccaggtccgc ctggcgggcc tggaggtcgc gctcggccgc
     1321 gtcgcgggcg gcgacggcgt cggtcagctg ccggcccagt tcctcgagcg aggcgcggtg
     1381 ctggtccagg cgctgccgcg cctcgtcggc gtcgtggcgg tcgcggtcgg agagggtttg
     1441 cagttcccgg atgcggtcct cgttgacctg gatcagttcc cgcgcgcggt cgaggtcggt
     1501 gcgcgcccgc atggaggctt ccatggcctg cgcgatggcc tgctcggcgg cggagagcgc
     1561 ggcccggttt tcagaggcct ggcgctcggt ctcggccatg tgcgcgcgga gggcctcctc
     1621 ctgctcggcg aagcccgcca cccgcttctc gagctgcgcg aggtcgaggt cgtaggcctg
     1681 catccggtcc cgcgtggcga acaaatcgag cccgcgcagc tgctcctgga tgacctggta
     1741 acggcgggcc ttgccggcct ggcgctggag ggagatgatc tggcggcgga cctcgcggat
     1801 gatgtccgcc aggcgcagca ggttggcctc cgtgtgctcg agcttgcgga tggcctcctt
     1861 cttgtccgcc ttgaacttcg tgatgccgct ggcctcctcg aagacctcgc ggcggtcctc
     1921 cgggcgggag ctcaagatct ggtcgatccg gccctgctcg aggatggagt aggagtccgt
     1981 gccgatgccg gtgtccatga acagccgctg gatgtccttg agccggcagg gcgtcttgtt
     2041 gatgaagtag ctgccctcgc cgtcgcggaa gacgcggcgg gtgacggtga cctcgttgta
     2101 ctcggtgccg agcaccttct cgcagtcggc gaaggtcatg gacacctcgg ccatgcccat
     2161 gggcttgtgg gtgtccgccc cgttgaaaat gcagtcctcc atcttcgcgc cgcgcagcgc
     2221 cttggcgctc tgctcgccga gcacccagcg gatggaatcg gcgatgttgc tcttgccgca
     2281 gccgttcggg ccgacgatgg cggtcatgcc cggctcgaaa tccagcttcg tgcggtcggc
     2341 gaaactctta aacccgatca gttccaggtg tttcagatac aaggctgttt ccccttcatg
     2401 cccggcggaa aaaaccgggc cggaccctga aaaatcaggg tgtattcagt accacgtgcg
     2461 gcacaggcgc gcaacatcaa tgcgacgccc gatcccgcgt tttttcgagg aattccgcgg
     2521 gattggaggt tgaagccggg ccggccttcg gccacaatgc atgacgggcg ccaaagcccg
     2581 gagcatggag ggtcccatgt acgttacgct ggtgcacgtg cgcgtgaaaa aagaggccgt
     2641 cccgtccttc atccaggcct gccgggccaa ccacgaagcc tcgaccagcg agccgggcaa
     2701 ccgtcgattc gacgtcctgc agctgaagga agacccgacc cgattcgtcc tctacgaggc
     2761 gtatgcatcc aaagaggacg ctgccgcgca caagaacacg ccgcactatg ccgcgtggcg
     2821 cgacgccgtg gcgggctgga tggccgagcc ccgcaagggc gtggtgtacg acggcctgtt
     2881 cccggcctga tcggctattc gccggccttt gtcgggtcgg acaggcggcc catgaagagc
     2941 aggctgccgg tctgcttttc cacgatcagg aaaaggaacg ggtggtccgc cgtgaacagg
     3001 accggccggc tgggcgccat ggccctcggc tgcatcacca cggccgtcgc cgccgcggcc
     3061 tcggtgcctt cctcgttgac ctcgacgaag gccttgtgga gcacggcgcc gatgtagagc
     3121 cagtccggcc gcccgtccat gcccgcgaag ttggcgaggg tgtcgctgaa ggcgtccggc
     3181 atgcccatcg cggacagcgt gtcgttcagg cggaaggcgg aggtcatcgt gaagcggggc
     3241 agcgtgacca ggacgtcccg gtcgctcatt tcatcgcgcc acgccccgag ccggtccgcg
     3301 gtgagcccct gctccagcgt ccgcaggccg tcgaccgtcg cgggcaggag gatcagcatg
     3361 gcgcaatcgc cgcccgcgta cggcagttcc gccaggtcca gttccgggaa gtgggcgtac
     3421 gcgcacttca gcttccggat catcatcggc gcctggacgg tctggtcggc ggacaggtgg
     3481 aaaggggcgt cccgcgtcgc ggccgcgtcg aacggcgcgg cccatttccc cttgaagtag
     3541 atggcgttga ccagcacgag gcgggtcatc gaggtgatga acccgggctg gatcaggttc
     3601 cggatcttct cccgcgtttt ctcctcgacc cacccgttga tccgggcccg gaccgattcc
     3661 ggctcgccga aatcgagcgg cgtcacggaa acgccgtact ctttctccag cagcgcaaca
     3721 tattccggga gaaacggata ctgctgctgc ggccacaggg agttggccgc ggtccattga
     3781 acctggcctt ccttctgtgt ccgggcgagc cgggcggaca ggtcggcgaa cgcggggtgc
     3841 agttcttcct ggccgaggct gaaacgaagc gtcgcggcca tctgcttttc cgtttcgccg
     3901 cgcgcgccgg cataggtcat ggcgagggcc gtggaaatgc tgtagggcga gaagaacaga
     3961 ttcccgggcg ccggggccag ggcgcggtag agatccacgg cgaacgcggt gttgtcctgc
     4021 gcgaggtccg cggccgcggg cagggcggcg gccagcgcca gcgcgaggat ggccgaaccg
     4081 acggggcgtg ctttcatggg tcctcctccg gagctacatc cgcggattca cgacgccgcg
     4141 ccggagcatc tggcgcagct tgcggagggc gtcgtcctgg atctgcttga tgcgggcgcg
     4201 cgtgaggttg agcatggctc cggtctcctc gagcgtccgg ccctcgatga accgcaggcg
     4261 catgatcagc ttcgagcgcg cggggagccg ctccaccgcc gtccagatct ccgagcgctg
     4321 cgccgcgtgg gaggcctcct cggcctcgct gtcctcgacg atgtcgtaca ggttggattc
     4381 gttgtccgcg aacatcgcgt ccagcgagac cggcgggccg ggctggtgct cgagctggcg
     4441 gacatacggg cggatcttcg ccacccgttc ctcggtcatg cccgtcaggt tggccagcag
     4501 ggcgtcgtcc ggcggctcgc cgcggtcctt gggccaggcg tcgaggattt ccttgagcgc
     4561 cagcatctcg cgcgagggcc gcatgccgat gctgccggag cggacgtggt cgcggaagta
     4621 gttgcgcagg cggttctgga cggccttctg cgcgtagacg tagaacggcg tgccgcgcgt
     4681 gaaatcgaac ttccggatgg cgttggacag ggccttggag ccctcctgca ggaagtcgcc
     4741 cagccagacc tggccgatcc agaagtaggg cttgatgcag ctcaccacga ggcgccggtt
     4801 ggcgatgaac agctcctcct cggccgcctc gatctgcgaa atgagcttgc gcgccgcgac
     4861 gcctgcctcg cgccactggg cggagctttt catgtggcgg cgggagagca gccggatctg
     4921 cgtggcgcac cagtgaatct cggtgaagag ggcgagcgtg gactccggcg tcagcagttc
     4981 caccgtgccg cgctcgatga gaaaccggcc gaccgcctgc atgttcgagt ccgggctcat
     5041 gtaggtgcgc ttctggcgca cgggcggggg gcagccggcg ggccggcgga actcgggccg
     5101 ggtgataacc ttgaagggaa ggccggcggc cagggctttc agctcctccc gatattgccc
     5161 gcctgttttc gtcaaggcca cgatcgtgcc cgatacggaa attgtcgcga acgctcgaac
     5221 gctacactgt gcatggcttg agccgcgctg tcaacggcgg cctgaagatt cccgcgacag
     5281 gagccgctcc gtgaccgccg ccaggacggg aagggcgggg ctgcggccga gggcgcgacg
     5341 gaggaggcgc agggccggcg ggatgaaccg ggcgaaggac tccaggcccg gctgggccga
     5401 cagctggccg tacgcgccga gggcctgggc caggcgttcc acggccgctg gccggaaaag
     5461 ccggatcgcc gaagggtcgc tcggccgccg ccgcgcataa tgcctgagca gcttctcctg
     5521 cagcggctcc ggcagggcca cgtagggatc gcagagcagc gaggccaggt cgtaggcggc
     5581 gggcccgaac cgcatgcctt ggaaatcaat aaacaccggc aatccgcgct tcagaaggat
     5641 attgctcgac tggagatcgc ggtgcagcag gacgcgcggc gcccggttca atcgagccgc
     5701 gatgccggcc agttcgcggc ggattcgccg gatttcaccc gcgctcgccc ggccggcgtt
     5761 ccgcaggaaa tgggtcacga acagctcgcg ctcccaccgg tacagcttcg gcgtaaaagg
     5821 ctgcatcaaa ggcagatgct cctgttgcgc tctcctcgtc cccgtttcgt gaagccgggc
     5881 cgcttggtcc aggattcgtt cgtacaagag ctcgatccgg ttcggccgcg agcagaggac
     5941 cacgtcccgc aggcaacggt ccccggcgtc ctccagcagg gccaggcgcg ccgcgggttg
     6001 gtccagccgc acggccggaa ccgggaaccc gaaacgcgcg aggaaccgag tgtgcgggac
     6061 ctgcagcgcg ttctccgctc gagcggaatc gtagcgcacg aggatcaggc tttggctgcc
     6121 gcgccggatg cgcgcgaacg cgcgggccga gccgcgcggg ccgaaaggaa ggaccgtcgc
     6181 ctgggccgcg ggccagccgt attgctgcag cgccttctgc tccgcgtcgt ccagcacgcg
     6241 gtccccgcgc ccggcgagga atcgaaccgg ctcgttcacg acggcatcgc ggccgacgat
     6301 ggcgtccgtg atgcgcgtgc ggggtccgag ccgggcgcct tcccaggcga cggcgttttc
     6361 gatccggcat cccgcggcga tccggacgga gcaagggatg gcggcccagc cttgcacgcg
     6421 ggcgcccgca ggccgcggca tacgggcgaa caggcggccg ccgggctttc cgtccaggcg
     6481 ggcctgccgg accgccgcgt gggcgtcgag gtactggcgc ggcgtgccga ggtccgccca
     6541 gaagctgtcc gggacgcaga cgcccgcgat gcgctcgccg cgcttcgtcg cgcgctcgta
     6601 ggcgggcacg atgccggcga atccctcggc cggcaggtag tccaggatgc gcggggagag
     6661 caggtgcagg ccgcagaagg taaacgttcc ctcggagccg gggaccggtg cgtggaacga
     6721 ggtgaccagg ccgttctcca tgctcaccgt ccgcgggccg agcgagggat gcagccacag
     6781 ggcggcgagc gggcgggcgc gtgcgaacgc gcggaggagc ggggccggat cgaggtccgc
     6841 ggccacgtcg gcgttgatca gccagaacgg ccgcttgtcc aggaaccagg ccgcgcgccg
     6901 cagggccccg ccggtgccga ggatctccgg ctcgaaggaa aagttcacgc gcaggcccgc
     6961 gaccgggcgg ccggcggccc accggaccag ctcgccgggc cggtgatgcg cgttgatcag
     7021 cacgtcgcgc acgccccagt tccggagcag gagcaggacg tgttcgagga tcggccggcc
     7081 ccacagcggc atcatgggct tgggcaggtc gcggctcaat gggagcatcc tcgtgccgaa
     7141 accggcggca agaataaagg ccttgctggg ggcgttcctg cgcatgcgtg caatgtagga
     7201 tattctgccc gcgaaaccaa tgccgggtcg tcaccgcggc gtttcaggct tgaccggcgc
     7261 ggccccggct ggaagcgtag ggtgcgacag gcgcgggagg accgtcatgg gcaagaccag
     7321 caaaaaaggc gcggcctaca cgcgggccgg cgtggatatc gacgccaaga tgaacgcgct
     7381 gcgcgcggtg cgggcggaga tccggggcac gttcacgcgc ggggtggtcg gcgacatcgg
     7441 ccatttcggc ggcctgttcc gcgcgccggg ccgcgaccgg ctgctcgtca gcagcacgga
     7501 cggcgtgggc accaagctca aggtggcctg cctcgccggc cggcacgaca cggtgggcga
     7561 ggacctggtc aaccactgcg tgaacgacat cctcgtgcag ggcgcggaac cgctcttctt
     7621 cctcgactac gtcggcatgt cccggatgaa cccggccgtg ttccggcagg tggtgcgcgg
     7681 cctctgccgc gcctgccgcc gcaacggctg cgcgctgatc gggggcgaaa ccgccgaact
     7741 gccgaacctg tacccggacg gcgagtacga cctggtcggc accatcgtcg ggtccgtgcc
     7801 gcggcgggaa ttgatcaccg gcgccgggat ccgccccggc gacgtgctca tcggcctgcc
     7861 gtccagcggc ctgcacacga atggatactc cctcgcgcgc cgcgtgatcc tcgaggaggc
     7921 cgggctgaaa ctgcgcgacc gcctgccggg cacgcgccgg accgcggcgg atgcgctgct
     7981 cgcggttcac cggagctatt tgaagcccgt ccgcgccctg aagcgggccg tgcccatccg
     8041 cgggatggcc cacatcacgg gcggcggatt tcccgacaac atcgcccgca ttctcccgcc
     8101 ggacgtttgc gcggtggtgg accgtgccct gtggcgcccg cccccgctgt tccaattcat
     8161 ccaagaggcc ggccgcgtgg accgcgacga gatgtaccgc gtcttcaata tgggcgtggg
     8221 cctggtgatc ttcgtgcgcg aggcccgggc cgtcgaggcg gtggaggtcc tgcgagcggc
     8281 cggcgaacgg gccgtcgcga tcgggcaggt ggagaagggg cggcgcggag tgatcctggt
     8341 gaattgagag gatgccatga agattcaacg ggccttgatc agcgtgtcgg acaagaccgg
     8401 gatcgtcgag tttgcgcgcg cgctgacggc gatgggggtc gaactgcttt ccaccggcgg
     8461 cacggcgaag tcgctgcgcg aggccggcgt ggccgtgcgc gaggtgtcgg acttcaccgg
     8521 cttccccgag atgctggacg gccgggtgaa gactctgcat cctaagatcc acgccggcct
     8581 gctctacctc cgcggccagc ccgaccacga ggcgaccatg acgaagcacg ggctcctgcc
     8641 gatcgacctc gtctgcgtca acctttatcc cttcgagtcc acggtcgccc gggacggcgt
     8701 gccgctgtgg gaagccgtgg agcagatcga catcggcggg ccttccatgc tccgctccgc
     8761 ggccaagaac cacgcggcgg tgacggtggt gaccgaccca tcggactatg cgcgcgtgct
     8821 cgatcagatg cgcgggcacg gcggcgacac gacgcccgcg ctgcggatgg aactggccca
     8881 aaaagtgttc gcgcgtaccg ccgcgtacga cgcggccatc tcgcgcttcc tcgcgaaccg
     8941 gctcgatgcg tcgacgccgc ctccgctggt gctgtcggcc ggcgatcccg tgaagctgcg
     9001 ctacggcgag aacccccacc aggaagccct gttctaccgc gagcccgacc cgcgggcggc
     9061 ctgcatcgcg cgcgtggaaa tcctgcacgg gaaggatatg tcgtacaaca actacctcga
     9121 cggcgacgcg gccctggagg ccgtgcgcga actctgcggg gcgcccgccg ccgccgtcat
     9181 caagcacagc aacccctgcg gatacgccac gggccggacg ctcgccgcgg cgctggccgc
     9241 ggcgtgggac ggcgacccgg tgtcctcctt cggcagcgtc atcgcggtga ccgttccctt
     9301 ttcgctggag gccgcgcagg tcctcaaggg ccgtttcgtc gaggcgctga tcgcgccgga
     9361 ctacgagccg gacgcgctgg agttcctgaa ggccaagagc aaggacctgc gtatcctgaa
     9421 gctcaaccgg ccgctgaccc cgcccgttcg cgaccgcgtc ctgcgccagg tccacggcgg
     9481 catcctggtg caggaccgcg acgtcatgga gatggagcag tgggtgatcc cgaccgaggc
     9541 gctgtttccc gaggagaagc gggcgctggc ggagttcggc atccgggcgt gcaagcacat
     9601 caagtccaac gccatcgcca tcgtccgcga gtacgagccc gggcagttcg ccctcctcgg
     9661 catgggggcg ggacagccca accgcgtgga cgccctgcgc aaactggccg tggcgaaggc
     9721 gcgggagaac atcgccctgc tggccaagca cgcgtccacc tacggccagt cgcccaagga
     9781 attcgagtgc cgcgtcatgg gcgagtgcgc gctggtctcc gatgccttct tcccgtttgc
     9841 ggacaacatc gaggccgcgg ccgaggccgg catccggtac atcgtgcagc ccggcggctc
     9901 gaagaaggac gaggaagtca tcgccgcctg cgacaagtac gggatcgcca tggccgtcac
     9961 cggcgtccgg catttccggc attgaagccg ggcgtcctct ccatgcgcct acggcgcctt
    10021 gagcgggcgc aggcggggcg gcagcttgcg cgccttctcg ccgctcgatt catccagcaa
    10081 gttccagccc acgttcgcct tggcggataa cgggtcgtat ttttccgcca ggaccgtgcc
    10141 gtccgtggaa agcacgcgaa tcccgtaccc cgcgatatcg tcctccaccg tgccgggatt
    10201 cagcgagccg gtccactccc gtccctcgag cttgaacgcc tcggtttcca cgacgaccgg
    10261 ctgcccgagc gccaggctga tgctcttgct cccgaacgtg cccgggaaca ggcgtccgcc
    10321 cgcgccctcg accaggatca gccactccac cttcacatcc gtcggcgtgg cgggcgacca
    10381 gttcttgagt tcgatagagt agcagatctg cttttcgacc gaccggcttg tgccgcgcca
    10441 gaggtcctgc tcgttgccct tgcgcgcatc cagggtcttt ttcgttgccg tgatctcgat
    10501 gttgtcccgg gcgcaggcca cggacgccag caggcccagg atcaatccgc acaggatagg
    10561 ccgcttcatg ggtgtccctc cctcgtggcg tccatcttag ttccgcgggc tggtcgctgt
    10621 ccagtcaagc cggaggccgg ccatctcctc gggtcggaga aaccgccagc cgccgatggg
    10681 caggcggccc agcgaaaggg ggccgatgtc cgttcggcgc aggcgcgcga ccggcaggcc
    10741 gaggacctcg aacatcctcc ggatctggcg cttgcggcct tccttcagga ttacccggca
    10801 gcaggtcgtc tcgctgcggg cctgaagcac gcgcacctcc tgcgcccgaa gcgtttcgcc
    10861 ctcatactcc actccttcga tgaattgccg cagttgctcg gccgaaggac ggccgcggac
    10921 ccagacccgg tatacgcgcg ggacgtggtg gcgcggatgc atcaggcggt gggccagttc
    10981 gccgtcgttc gtcaacagga gcaacccctc gctgtccgcg tcgagccgcc cgatcgtgta
    11041 cagccggccc gcgggccacg gatcgtccga gaccgaccgc gtcccggcca gggcgccgac
    11101 cacggtcggc cggccctgcg ggtcatggct tgtgcagatc agcccgatgg gtttattgac
    11161 ggcgatcacg acgggcgcct cggggcggac tgcgcgaccg tccgcttcga cccgatcgcg
    11221 agtcggatcg atggtggtcc cgaggcggcg gacgacctgc ccgttcacgc ggattcgacc
    11281 atcggcgatg agttgctcgc aggcgcggcg cgagcctatc ccgcactggg cgaggtactt
    11341 ctggaggcgc atcatgcttc gagcagggcg cggacaagcc ggccggaaac ccacttgcga
    11401 tacgtggcgg tggaccggat gtcgtcgatg ggatgcacct cggccgacgc gcggcgctcg
    11461 acttcctcga gcaggttcag atccacccgg cggccggcga tccattgctc caactcgggc
    11521 aggcggaccg gcacgggcgc gacgctgccg agcgagacgg ccagcgaaac gatcgtcccg
    11581 ttctgaacgc ggacgcggca agcgcccatg accttggata tggcctgcgc ggcgcgcgtg
    11641 ccgatcttat ggaacttctc ccgcgcgccc gcgggcaggg ccgggagcgt gaaggagacg
    11701 agcagttcat ccgcgcgcac gtcgagcttc cggtagccgg tgaacagctt ggccagcgga
    11761 atgtcccgcc gtcccgaggc cgaggccgcg gtcgccagcc cgtcggtgat cagcagggta
    11821 ggggcgaggt cgcctgccgg gctggcgttg gcgacgttgc ccgcgatggt gccccggttc
    11881 tggatctggc gtccgccgac cgtcgccgcg gcggccgcaa gcgccggaag cttttcccgg
    11941 acaaggggcg acgaccgcag ttcggtatgc gtcgtcatcg cgccgatctc gatgccgcgc
    12001 ggcgtttcgc ggatgccctt caattccttc agcccccaga ggctgaccgc gcgttcggga
    12061 atggccgcga ccccggattc ccactggacc atcaggtccg tgccgcccgc cagcggacgg
    12121 ccccgcgtct tctcgtccgc ctgccacgcc agcgcctcgg ccaggctgcc cgggaaccgg
    12181 atttcgatgt cttccaccgt tctcattttt cgggccaccg gttggcggcc agcttgaccg
    12241 cctcgatgat tttctcgtaa cccgtgcacc ggcagatatt accggcgtgc gcctcgcgga
    12301 tctccgcgtc gtccggcgtg cgcgttttcg agagcagcgc gaaggacgac atgatcatgc
    12361 ccgggatgca gaacccgcac tgggccgcgc cgcgctcgat gtaggcctcc tggaggatgc
    12421 gcccgccctc ggttttctcc aggccttcca cggtcatgat ctctgcgccg tcggggacct
    12481 gcccggcggc gatcaggcag gacgtcacgg gttcgccgtt cagcaggact gtgcaggccc
    12541 cgcactcgcc ctcgccgcag ccttccttgg ttcctttcag tcccaggtcg ttgcggagcg
    12601 cgtcgagcag acgccgcccg ggcgggagat ccaccgtcca gcgctcgccg ttaacgaccc
    12661 aggttctttt gactgtgctc ataaagtttt tccggcgtga tgggcaggtc gcgcatgcgc
    12721 aggcccgtcg cggcctcgat cgcgttggcg atcgccggcg cgaggccgtc catcgggagt
    12781 tcgccgaccc ccttggcgcc cggcggcgcg tggtcgaagg ggaactcgac gaagtccagg
    12841 tccatttcgg ggatgtcctg catcgtcggg atgatgtacg tctgcatccg gctggcgtcg
    12901 aaccggccct tcctgtcgat gccgattttt tccatcacgg aataaccgag cgcctggacc
    12961 aggccgccct cgatctggcc cttggccagc acgggattga cgaccttgcc aatgtcgtag
    13021 aaggcggtga ttttcttcac gcggattccg agcgtccgcg gatccacctc gacctcggcg
    13081 acgttgcagc cccacgagta gccggggtag gagtcgcccc ggaaggtctt ctggtcccac
    13141 tgcgtgcccg gcggcaggac gaacaggtgc tccacgcgca ggcggccgtg cttgttcagg
    13201 taggcggagg cgatctcgtc gaactccttg cccgacgcaa tcggtttccc ggcaaactgt
    13261 tccagcgcct ttttcagctt gcccgcggcg ccgtagacgc agctcccgac gaccatggtc
    13321 gtgcgagagg cgacggtcgg gccgctgttg ggcacgaggg cggtgtcggg gaaggggcag
    13381 ctcacgcgcg agaggtcgag gccgagtccg tcggcggcga gctggctcaa gacggtcagc
    13441 gcgccctggc ccatctcggt ggagctgacg cggacgatgc cgccgggccg gccgggcgcg
    13501 agccagtcga ggtccatcgc ggcgcgggcc ttgatgcggg cctcgccgtc gccggtgaag
    13561 gcggcgccgt gcgcgaagaa ggagacgccg atgccgtacc agctctttgc gccgggcttc
    13621 ccgcggctgc acttcttcca ctgctcgtcg aaccggcagc gctccagcga tttctccagc
    13681 accgcgggcg agccgacgct ccatttcagc acctggcccg tcggcgtggt gtcgccctgg
    13741 acgagacagt tcttcagccg gaactcgtgc ggcgtcatgc cggcggcggc cgccatggcg
    13801 tccacgtggc tctccagtcc ccagatggcc tgcggcgcgc cgaacccgcg gaacgcgccg
    13861 gagggaaccg tgttcgtgcg cgcgacgagg ccgttgacga agacgtggtc gcagcgatag
    13921 cccatcgccg cgtgcaggat gccgcggtac atgaccacgt cgctcaaggt caggtacgcg
    13981 ccgccgtcga gcaggaaatc cacggcgatt gccgcgatcg tcccgtcccg gttcagcccc
    14041 gtccggtagc gggaccacac cgggtgccgc ttggtcgtgt agaggatgtc ctggtgccgg
    14101 tcgtagacga tcttcaccgg ccgttttgcc ttgagcgcga gcagggcgac gtagccggcg
    14161 agccaggtcg ggaattcctc cttgccgccg aacgcgccgc cgacggcggc ctgcttgacg
    14221 cggatcttct cgggcggcag ggcgagcgcc tcgcacaact cgtgcacgat gaagtacggg
    14281 cactgcatgc tgccgtagat gaacacgccg ccgtccccgg tcggctcggc gacgaggccc
    14341 tgcggctcga tgtagagttg ctcctggtgg cccgcggtgt actccgcctc gacgaccttg
    14401 tgggcgcggg ccagcgcggc ctcggcgtcg cccttgcgga tggtctggcc gcagagcttc
    14461 acgagggccg ggtcgttatg cttgaactgg tccgcaacct cgtgcagtgt caggatcgcg
    14521 ggccgttcct cgaaatcgat gcgcaggtgc tccgcggcct cgcgggccag ccgaagcgtg
    14581 ggcgcggcga tcagcgcgac cggctcgccg gcgtacagga actcgtccga ggcgatgacc
    14641 ggcatcgagc ggtcgtggct gacgatgcaa ttggggccgg ggatgtcctt cgcggtgacc
    14701 acgaccacgc gcgaccagtc gaacgccgga tcgggcgtca gcccgcgtag caggccgtgc
    14761 gggacaggcg cgcggacgac atggccgtgc cagccgttgg gaacgaggag atcgtccacg
    14821 tactgggcgc gcccgcggac tttatcctcg gcgtccacgc gggggatgcc ctggcccacc
    14881 catcgaggat cgcgtgtctg cacagtggcc ggcatagatc gctccaaccc ctgcaattta
    14941 caccagcccg ggcggttttg aagcagaaaa aagcgcgcta gaccgtggcg ggggccggcg
    15001 cgcacgcaga agcggcggtc gccggctccc tgccccgggc cggattccac ccgcgtatct
    15061 tccaccggaa cgtgcgcccg tagtaggcca gattcacggc cgccaggtac ggaagcgacg
    15121 ccaggccgcc gggccggaaa gcgtgccgcc acatgcggcg ggccatgcgc caggggcggt
    15181 agaactcgcg cgtgacccag tcatgtcccg cctgcaggtc ggctgccgtc atgttttgcg
    15241 gccggaatac cgcgtggtgg aaatcgtagt gcgaccagtc gccgtccatg atccggtctt
    15301 tcatcatccc gtgccgcggc gtaccgggca ggggggtgaa gatcgaggcc tggatcacgt
    15361 cgatctccag gtcgtcgagc tgcttcagcg tggcggcaaa gacgtccggc gtgtcgccgt
    15421 cgaacccgaa cacgatgccc gcctccacgg cgatgccgtg gcggtgcagc aggcggacgg
    15481 cctcgcgata tccgctgacg cggttgaacg atttgctgac ggcgccgagg ttccggtccg
    15541 agaacgtctc cagtcccacg aagacgccct tgcagcccgc tcgcgcggcc agttccacca
    15601 gttccggatc gtcggcgatg gccagcgtgg cctgcgtgat ccaccatttc cgcaggggag
    15661 ccagcgcctg gaacaggcgg cgggcgtagt cgcggtccgc cgtcaggttg tcgtcgacaa
    15721 agatgatgaa cttgtcggga atggcggcga cttccgcgac cacctcgtcc acggggcgct
    15781 gccggtgggt cttccggttg aacgcggaga ttgcgcagaa gtcgcagccg tgggggcagc
    15841 cgcgggtggc ctgcacggca tggaccgtcg cgtagcgccg gccctgcagc aggtgccggg
    15901 gcggaggatg caggccgcgc agcgagtgca ggccgtcggc ccggtagatt ccgctcatcc
    15961 ggccggcgcg cgcgtcatcg accacgcggg accacagtcc ctccgcttcg ccggccagga
    16021 cggcgtccgc gtgccggatg gcctcgtcgg ggcagaggga cgggtgcatg ccgcccagga
    16081 ccacgggcac gccgcgcgca cggaaccggc ccgcgatctc gtaggcgcgg ggcgcgagcg
    16141 cggtcatgca cgtaatgccc acaaggtccg cctccgcgtt ccacgggata tcctcgatct
    16201 gctcgtcgac gatacggatg ctcacgtccg ccggtgtttc cgccgccacg ctaagcaggc
    16261 ttaacatgga gaatcgaaat accttgcccc cgaatagcct gttcttcccg acgctcgtcc
    16321 accggccgct cgccggcgct atcaacaaga gtttcatgcg atgtcctttc cgttgtccct
    16381 tgtgcgcatt tcttgatagc agctagcctg ccaaccggaa ggcgattatt cacaacatgc
    16441 ttcatatgag atggttgtag aaagttcacg cgaatgcgcc tgcctagagc ttgtttggtt
    16501 gcattcattt tatgtatact atacatacat tgaatgtatg aagcagagcc ttcgaatccc
    16561 ggcctccgat cagccgctgt tccggttggt ggaggcggcc gcggtggcca atccgttcag
    16621 cgacgaccgg ttcgaggtgg agcggaaaat cgccggcgcc gcgggcggga ccgccgcgcc
    16681 aatccgcgag cgcgccattc aaaaggtacg cgagcgggtc gcgggatacg tccggcgagg
    16741 ccgggccgac atccggctct atccgcgcga ggacgcgcag cggctccgga cggtcatcct
    16801 gtttgacgtg ttccaccgcc atatccagga actggacggc ctgatcgtgc ggcagcagga
    16861 ggcgggtgac gccccgatcg aggtgccctt cgcgggaacg attcttcagg agatgacgga
    16921 cgccgggttg accctcgatg cctcccgcca tgtcctggcg gaattctacc aactgcggcg
    16981 cgcgttctac ttcatccgcc acgcggcgct gggctccagc ccctgcatgc aggcgctgcg
    17041 atgcgagctc tggaccaacg tcttcacgca cgatttcaac ctgtacgatc gcttcctgtg
    17101 ggatcgcatg gaggatttct cgacgctgct gctgggcgaa acaggaacgg ggaagggcat
    17161 ggcggcgcgg gccatcggct gctccgggtt catcccgtat gatccggtga aggggcgttt
    17221 tgcctgcagc ttcacccgaa cgttcatctc cttgaacctc tcgcagttcc cgccgtcgct
    17281 catcgagtcg gagctcttcg gccatcgcaa gggggccttc accggggcga tcgaagagca
    17341 taccggcttg ctggcgcggt gcagcccgca cggcgccatc ttcctggacg agatcgggga
    17401 cgtggacatc cccatccaga tcaagctgct gcaggtcctc caggagcgga ccttttcgcc
    17461 cgtcgggagc cacgagaaga agcggttcca gggccgggtc atcgcggcga cgaacaagcc
    17521 gctggagcaa ctgcggcgca gcggccgttt ccgcgacgac ttctactacc ggctctgctc
    17581 cgaccagatc acgttgccgc ccctagcgca gcgcctgcgc gagaatccct cggaactggg
    17641 cgatctggtc ggcgccatcc tccagcggct ctgcggaaag agcttcccgg aactgaaggc
    17701 cgtcgtgctg gccgcgctcg aaaggagccc cgggccgaat tacgcctggc cgggcaacgt
    17761 gcgcgagtta gagcaggcga cgcggcgcat tcttctcaag gccggatacg agggcgacgc
    17821 gaagcgggcg gacggcggag cggagcccga actgctggaa gggatccggg cgggaaccgc
    17881 cgacgcggcc tctcttctct ccatgtactg caaggttctc tacgagcggc acggcaccta
    17941 cgaggaggtg gcgcggcgca cgggcctgga ctggcgcacg gcgaagaagc acatcgccgc
    18001 ggtcccgtaa gcgcatatcg ggcttgtcct gccgggcggg ggtgtggcat cgtgccgcca
    18061 tgtcaaatcc ttcgctcctt gtcctggccg tcctgctgct cgcggcgggt tgccggtccc
    18121 cgcgggtgga acagatccac ccgatgacgg aaggggagat gcgcgccgtc ttttcgcgcg
    18181 cgcaggatcc gcggcggttc tggatcacgg tgtacggttc ggaggaaggc gcgtatttcg
    18241 ccaatgccaa ccggctgcac ccggaccagg tcgtggcctt gcccttcgcg gccgggaagg
    18301 cccagccgga ctggcccctg gtcggcgtgc aggcgacctc caaggagtcc ttcctggcgc
    18361 tggtggacac gagttcgcgg gacagttggg gcgcgcccga tatcgtgctc ggcctgcgcg
    18421 gcacgccgct ggggccgccg gcctacgacg ccgttcccga gcatgtcgcg gagccggtca
    18481 aggggtacgc ctgcgtgatc ccgacgctcc ggtacgacct catgcagatg gaaaacgtgc
    18541 tggtctacct gcgcgccgcc caggggccgc tgggctacct ggcgcgccgg gacgagaaga
    18601 agacgccgcc ggtcctggtc ctgggcatgt cgtgggtcaa cgtgttttcc ttcgtgcagc
    18661 tcaactatcc cgaaaaactc ctcttcctgt cctcctcgtc ggcctacggg ccgtccgccg
    18721 accacctgat cgccgaggtg ccgctgaaga cggtgcgcgg ggcgctcgcg gtggacggcc
    18781 tcatcgatgg ggagccggcc acgctggtcc tcgattcggc cggcgatttc gagctggcga
    18841 tgaccgagcc gccggtcgag cccgtccgcg tgagcgtggg cgacctggtc tttccccgcg
    18901 cgaaggccgt ggcggcccgg gagcacgcgc tgggcctgcc cagctatccg cggatcggcg
    18961 cgaagtggct ggccaagtac aaggtcacca tcgccccgaa ggccaaggtc gtctacttcg
    19021 aacggccgtg acggatgacc gggatcggcg attccggctg catcacggct ccgccacgct
    19081 gatcttgtag aaccggttgg tcatcaggtt cgggtgcggc gaggtctggg ttccgttgtc
    19141 cgtccactcg tacagcgcgc cggtcccgtc gatgccggtc ggggtcgcgt tggaccacga
    19201 ctggttcagc aggccggtgt tgttgtagta gatgtagtac ttccggccgt tcgccgtgcc
    19261 gaaccgcacg atgaacccgc tggagttccg ggcggtcgag gcgatctcga agaagttgtc
    19321 cttgtccttc ggatcggtgt ccgccacctg ctcctgccag tcgagcaggg tatcgtcgtc
    19381 ccagtcgctg tcctcgtcgg ccgtggtcag gttgccgaag ttatcgagct cccactggtc
    19441 ggggatgccg tcgccgtcgc tgtccgtgtc gccggtgccc aggacggaga agcccatcga
    19501 gaaggcgaac gcgttggtgc cgaagacctc gacctggtgg gtctgggcct ggttgcgcat
    19561 ggtgatggtg aacacggcgt tggtgcccgg catatcgtcc acggcctgca ggttgaaggc
    19621 cgcattgtgc ccggcgtcgc cgtacccgag ccgggcctgg acggtcttgc cgccgccgag
    19681 gttggccagg gtcatgacgc cgccggaatg ctcctcgacg ccgagggtgt tctggccctg
    19741 gcggaggagg ttgtcgaggt tgggcgagaa gccgtggcgg acgtagagca cgccgccgtt
    19801 gagcgtgttg gtcacgttgt aggacacctg gaagaggttg gtgccctggt tcagggtgat
    19861 gatcttccgg atcttgccgt cggccgaggt catgcgccag ccgttggtcc agtaggtgaa
    19921 cgtgtagaga tcgttcacgt agttcgtcgt gccggcccac cagtccttca ggcccgaggt
    19981 ccggtaggag agggtcgtgc cgttggtggc gacgtggccc gtgccctcga cctcggtctc
    20041 gacgcccgca tagctgacgg ggttgccgag cacctggcgg acggcgccgt cggacgggtc
    20101 gcgcgcgaac gcggcggtca tgcggccgcc gatccgctcg aagatcgcgt agaggcggtc
    20161 gttgtagagc acgtactcgt tctcgccgtc gaggtccaca tcggcctgcg cggcctgcgg
    20221 ggtggtgacg ccggaggcga tggcggccca gtcgtccacc tcctcgtaca gggcggcgag
    20281 ccgggtctgg gcctgggcgg tcttggcgaa gtcgatcagc gtgaggtagc tctccgaggg
    20341 gtagaggtac tcgcccgtgc tccaccggcg caggtcgttg ttgtcctcgt tgtggaaagc
    20401 cgtctcgaag accgaggcgt ggatcaccgc gcgggcgagg cgggcgatgt tggtgtccgc
    20461 gatggactcg acctgtctcc acgcgctgct gaccgcgccg ccgaagtaga gcatgccgta
    20521 ggcctgggag acgttcgtgc cgggccggat ctcgaagacc ttgttctgca ggccctcgcg
    20581 cttggtcgcc tcgccgacgt accagttgtc gtagtcgccg cgggtggcgt ggttgatcca
    20641 gtcgtggctc tgcttggaga gcgacggcga gccgcggtcg ttcttccacc aggcgttgcc
    20701 gacgtcgtcg ccgtcggtgt cgatcgctcc ggacaggacc tcctcgaggc ccaccagctt
    20761 gatccagggc cggttggcga tccagcggag gttgcggtcg tacgcgtccg catcgtcgtt
    20821 gtcgctgaag tcctcccagt tgctgaagat cgtcgtcacg cgctgctggt cgccgcgggc
    20881 gcggcggttg aacagcgcgc gcatggacag gctcaacccg ttgtcggtgt tcgagaaccg
    20941 gtactcggtg ggcaggttgt tgatgacgaa gcagtccacg ttgccgatgc ggttgatgct
    21001 gtagccgttg tcgcccatcg agtcggtgcg gccgaaccag tagtacaggt gcgtgttctg
    21061 gtcgagcacg gtggcgcgga acccggcgtt ggtgatcttg gcgagcgtgt cgtcgtccag
    21121 cacgcgctcg ggcgtccaga acacgctgtt ggtggtgaag ctcgcgccgt agatctccga
    21181 gaggtattcc gaggccagcc ggatgttgtc ccggttgaac tccttggtga agtacgggag
    21241 gatgtggtcg gagaacgtgc tgccgagcat ccagacgacg ttggtcgcca cgaggcggcg
    21301 gacggtctcg ttgagcgtcg gcccgtcgcg ccaggtcggg ctggcgttcg tgtccacgcg
    21361 cgcccactgg agggaggccg ccagcgtggg cgtgacgtgc aggttcagcg cctggccgaa
    21421 gacctcgtgg gccagcagcg ggcggtggta gccggcgccg gcgcccgtgt tgaccagcaa
    21481 ctgggcgacg tggccgggct ggtcgtgctg gttgccgtgg aggacgatcg cggccttggc
    21541 gcggccggcc cggaccgtgc cggggatcca gttgtacagc acgctgttcg cgccgctcaa
    21601 gccggcctgg gcctcccagt aatcctcggc gatgtagtcg ttccagatcg cgtcgcggat
    21661 gtcgctgcgc ccgccgaggt cgcccgcgcc gacggggtcg ttctgcgtcc cgtcgcgcgt
    21721 ggagtagacc tggaagttga gcgaggccat gctgccgttc cagccgatcg cggtcagcgc
    21781 ggtccggctg atcgagaact cgacggcgtc cagttcgtgg ttgtagtagg cgctgccgaa
    21841 gtgctggggc tgggcctgca cgccgccgta ggtgaacagg tcctcggcga acgtgctcgt
    21901 gttctggccg gggttcgtgt cgacgtacac cgtgcccgcc gacgcatcgt agagggcgac
    21961 gaccgcctcc cagcgcatct cggtcagcgt gtcgacgtcg tccggcagct tgcgctcgcc
    22021 gatcgactgg ttgcccgtgt cgatgacgac gtacaggtcc aggttgccct gctcggccag
    22081 cgccttgagg tcgtagaagt cgaagcggaa gtacagcttg ccgtcggacg agccgccgtc
    22141 gcgcgcgtag aaggcgagca ggtcgcggga ggtgtcccag tcgtcccagg ccttgtagat
    22201 gtcgccctgc tggttgttgt tgccgccgcc ctcgtagtag tcgagcgccc gcaggtccga
    22261 ccaggtccac tccgtgaatt cctggtagtt ggtggtgccg ttgatcacgc cgagggcctc
    22321 gccgcggccg atgacgatcg agccttcggg cggcggcggg ctggtgacgc acacccgcgg
    22381 gtcggttccg ccgagatagt gctggtagtt gctgaccccg cagctggtca ggtcgccgtc
    22441 ggccccctgc ctcgtgttcg tcgggctcat ctggccggtc tgaatgctgt accagccgtc
    22501 gttgaacgga tcgaggccgt accgctgctc ccacccgtcc ggcaggccgt cgtcctcggt
    22561 gtcccggtcg agcgggtcgg tctccaggac cagcaggcgc ggccagacat tcgtgttctg
    22621 ccagacgccg ttcgtgtagc gcggccgcgg gtggtccgtc cacagcttgc tgtaatccaa
    22681 cgcgcgggag cggacgccgg agagcccgtt gccgaggatg ttcgtgctgt tgttggtcca
    22741 cgtgacgacg aacgtcgtgg tcgtgccggc gggccagagc agcgccaggt tcacccgctc
    22801 gttcaggtcg gcgtcctcgg cgccgtcggc caggtcgtcg ccgtccgtgt cgccgttgtt
    22861 gggatcgggc tccaggaagc gggcgttggc ggccagcgaa tcgctgtcca cctgggacga
    22921 gttatacacg gtcgggacgt tcggatgggc cagcacgccg gtggccacac cgctgccgtt
    22981 gagcgtgccg atttccaccc ggccgttgcg gttgaggtcc tcctgcccgt cgagcagccc
    23041 gtcgtagtcc gtgtcgggct tgctcggatc ggtcgtcgtg ccgcagatct ggtccgtgcg
    23101 cgaccggttg aagttgtagc gcgagtagcc ggagttgtcg gtggtgttgt agatcgggat
    23161 gtcctggtcg gcgatgaagt tcttccaccc gtcgccgttg gtgtcggtgt tggtatccgt
    23221 gccggagctg gggccgcccc atccgctctc caggccgtcc gggagcccgt cgttgtcggt
    23281 gtccgggctc aagggatccg tccggccata gacgtaccag atgtggacct ggccgttgtt
    23341 ccaagtctcg ggattcgtat ccgggaggtt cgtcgacgta acctcgggat cgtcctgcag
    23401 cccgtcgtcg tcgtcatcca ggtcgttggt gttcgagttc accgattgca tcagcaccac
    23461 ggtcgcgttg cgcagggcgg aggcgtgctt ggtcgtgccg gaggccgagt tggtgtccgc
    23521 gtgggcgtag aactggaaat ggccggcggt catgttggtc cacaggaagc cccagatctg
    23581 cttgttcccg gagaccgagg ggttcgggtc cacgcgccac gccgtctgcc cggaggccgt
    23641 cgcgcccggg taggtcgcgg tcaggatcag ctggttgctc gcgaccacct gcgagacgac
    23701 caggtagttc gtgctgatcc ggatcgtgtt gccgacgccc agctcctggt ggaacagcgt
    23761 gccggacccg gcgaccgtgt tggatcccgc ccacacggac accgtgcccg tgagctggtt
    23821 gctcgtcgag gcgatccgcg gcgcgaagcc caccgagttg gtgaactcga tccagacgtt
    23881 cctcgccttc gtgtccgtct cgacctggac ccggtactgc cgctgcgtcg gcgacgggct
    23941 cgccacgtcc ggcaggacga tctcgtacgg cgcgccgtcg gagtcgtact cgggcgggtc
    24001 ggtgatctgg atcaggatgc tgtccgcgct ggactgggcg cgcacgatgc ggctggcctg
    24061 cagcgtgatc cccagccccg ccgcgttcgt gtgcaggacc tggaggttgt gcaggtagtt
    24121 cgtgtcgccg ttgtacatgt ccgacagcgg gatggccagg gcgtggtggc tgccgctgac
    24181 gtcccactcg aagtaccagt cgtcgcggcc ctgggccacg tcgttgatct tgaagatgaa
    24241 ccggctcttc aaggtgtcgt tgtcgatgcc gtcggcgagc gactggctga accagacctt
    24301 ggccacgtag ccggagaaga ccaggtcgcc gtcgtactgc ggccacgcga cgtacaggtc
    24361 gtaggccggt gcgtcggtct tcacgttgcg cgtgagcgtg gtgaagtggc cgagcgtgtc
    24421 cgacagggtc aggttggtcg aggaggagag ctcgcgcagc ttgaccgtga tggtcgcgtt
    24481 tgagttgccc gacgggatgt tgcggtaggt gaaccgccac tcgtcgggat acgcgctcga
    24541 gatgtagagg ctcggcgtca ccttataggc ccgcgcccac gagagctggc cgacggcgtt
    24601 ggtgccgatg ccgttcgtct ggcccgtgga tccgtcgtcg ttggtcttgt cgctgtcgct
    24661 gatgttgaac cagacctccg tcaccgtcgg gtcggtccgc acgaccgcgc cgtactcctt
    24721 cgagcccagc gtctcgctct cggccgggta cttcacctct ccgccgggcc ggtccatgtc
    24781 gaggtagaag ggctgggtga aggtgttgaa gatggacgcc ttgccgcccc ggtccaggaa
    24841 cgcgcgcgac cgcatgacgt ggaagccgtc cacgaggccc gtcgagaccg tgccccagtc
    24901 attgtgcggc cggtacgcca ccgtccgcag gttgatgttc gtcacctgcc acacgcccat
    24961 ccgggtcgtc ttcatggaaa cggaggcgcc gtcgacgggg ctctcgacat cccaggggaa
    25021 gccgtccgag ccctgttcgc ggtaagcccc gaccttgtac cgaagcaccg tgccgttcgt
    25081 catggccggg atcgtcgccg accaccagtc cggcgtgccc gtgccgtcgg gcgccccgtt
    25141 ggcgtaccac gtcagcggaa cgaccttcgt cgtgccgcgc ccgaagccgc ccgcgccctc
    25201 cggccacgtc gcgccgtcgg tcgtgtaata cagcgccagc cggttgacgt acgtggacga
    25261 gtagcccacc ttcacgcgga ccgtgatgtt ggagttgacc gcgttttggg gcagggggaa
    25321 gaactgcttg gccgaccact cgtcgttatc gttgggatta tggtacaccc aggccgcggt
    25381 ctccgcgccg tcccagtcgt tgttcccgct gcccgcgttg gcataaagcc ccgcggtgcc
    25441 gatcgtcatg gtatagcttc ccgaacccat cgagccgatg gtgttgtgcg ccgagttcgt
    25501 gtccgcgaac ttctcgcggt gctgccggtg gatgaacagg gcctgctcgt acccctcgaa
    25561 cacgtccgtc gagccctcgt tgccgggcgg atggtcgcgc gggtcgccgc tgctgtggtt
    25621 gttggtgttc aggttcatcc cgccgtccag cttcatcagc acgttcgccg ccgagccgtc
    25681 gacgaagctg acgaaccgga gattggtcga cgaggtcacc cgggggaggc tccacgtgta
    25741 ggagtagtcg gtgctgttgg cgtccgccgc gccgtacgga ttgaagcccg ggtcgccgtc
    25801 cggcccgtcg cgccgggtca ccgagatggt cccggcctcc tggccgttct ggaagatcgt
    25861 gaccgggctg ccgccgtggc agctccaggg ctgggcctgc tccgggctgc gccaggagaa
    25921 ggcgaagtat ccgcccggcg ggacgatcac cgatccaagc tgggaggcgt aggtgtagaa
    25981 ccccccgccg taggttgagt agttgtagag gtacgcgtcg tccacgaacg gggtgtggcc
    26041 gaagccggtg gagatgctgc gcgactggcc ggaggcgtag ttgtcgttga tcatcatcag
    26101 cagcaccgtg ccgtccgggt cgttcatgct gccgttctcg cgcttgtcca tgcgctcgta
    26161 ggcgcagaag tcgccgtccc cccatttcga catgtgccag ctgcgcgcga aatggctgtg
    26221 gatgtacgcg aggttcggga tgcggctgtc cccccactgg cccaggtagc aggtgttggc
    26281 gtggcggggg aacgcgccgc cgctcgcgcc catgatctcc gcgtggcggt tgccgtccgt
    26341 gtagatcacc ggcaggcccg cgcgcgtcag gttgaacgcg ttgtgcaact cctcgcggta
    26401 ggcgacgttg tcgtcgtggc tcttggcgta gtacacgccc aggtactggc ccatctgttt
    26461 gcccgagatg tagtccgccg aatcaagccc cgagagcgat ccccaggggt tgccgaggtt
    26521 gtcgttgaac gtctggtggg tccgcgcgtc caggaggcgc atgccggcgg cccagtagtc
    26581 gccgtagggc ggcggctcgc ccatgtgctc gccgaacatc agcgcgtcgt tgcggccgaa
    26641 ggacttttcg agatcgaaga cggtgtcgcg gtgattgtcc cagtcggcgt agccccgggt
    26701 catgttgaac tgccactggg cctggccgca gtagcccgcg ctgctcgaat ctttgtcgcc
    26761 cgcccactgc tcgccgaaga agtaacccgg cacgtgcttg acggcgtcga ggcgcaggcc
    26821 gtccaccttc gtgtggtgaa tcagccagcg gacgttgcgg cagaggtacc cgccgacgtc
    26881 ctccgtgaag gagcccgggt actgggcgat catgttggtg gtgatgttcg tgctgtagaa
    26941 tcccacgtag gccatgccgg tcggtgtgta ggcccagtcg taccactcgg ggtgggtcgg
    27001 ctggcggacg aagtggatct tcgggtacgt gctgccctcg gacgcgccga agttggcgtt
    27061 cgggtcctcg tgcgcgatgt cgatcaggtc ggacaggttc cggttctgca cttcccacgt
    27121 gctgccccag ttcaccgtgt tgtcccactt gcggtagaag ccgtcctccg tcacgcgcag
    27181 gtggaaatcc tcgggggcca tgccggggta aatgtccagc gccgtgtact cgtcccagcc
    27241 cggcacgtcg aacgcgcggt ggttcatgat gttgtcgaag tacacgcgca tgccgaagcg
    27301 gtgcgcgagt tcgatcaggc ggagcagttc cgcctctgtg ccgtaccggg tcttcaccgt
    27361 gccgcgctgg tcgatgccgc ccaggtcgaa gggatcccag aggtcgtagc cgacggaaag
    27421 tccgccgctg cccttctgcg ggggcggcag ccacagcgcg ccgtagccgg cctccgcgag
    27481 ttcgggcagc ttgtcggcga tctcgttcca ggtgttgttg aaatactgca gggctacttc
    27541 ggcgggggcg gacaagctcg cgagcagcag gagtgaaaac gcgacggttg cggcgcggat
    27601 ggaacgagtg ctcatgtgcg cttccccttc agataagtta cctcaaatcg gcatgggttg
    27661 aaagccgctt ggcgacggtt ttcatcagcg aaacagactt tctccacccc ggtattttca
    27721 ccataccctc gtcagtatgc cgtgttaagt gaagcgcttt acttttctgc tgcaggccgt
    27781 ggtatggtat tgcctggcgg caaacacaac ctaggagaag ggcgcaatca caaaaacgct
    27841 tacgtcccaa agcgcgtgac tgtatataat tatggaagca gaagatgtgg tggcataaag
    27901 ccatccccag aagagtccag ctaccagcgc ggcgagaatg agaaccaggt ttccactacc
    27961 ggcatggacg ccggcataga gcagggcgcc aatcaggagg cctgttcgat cgccgaatcg
    28021 cgcacagaag cgtggctgaa gaaaaaggcg ccagaaaatt tcttcgccgg ggccgatgat
    28081 ggtgatcatc agtaaagcga ttaaccacgc ggacagcgcg ggccgcagaa ggtagacgct
    28141 ggatatgtgc tgctccgaat cgggaagcca tccgcggatc agcatccggc cggcgaggaa
    28201 gatcaagtag aggcccgccg ctgaaacaat gccgatgaca agttttctga acgcatcgga
    28261 tcggaagtcc gagaccagtt gccggcggag gtcggggcgc gaaacgagca tggatgtgtt
    28321 gagcaggaga agcaggcctg acatccacca ccagaacagg gccggcttgg ccagacccca
    28381 tcggaacagc ggagcgaaaa gaacggccgc aactcctgcc aggaggacga tgggtatgct
    28441 cttcgccctc gcggaagcgc gcagcatcag aggagcctgt ccaggatgaa gcccgcggtg
    28501 cagagcagtc cgaacgccag gttcaactgg gccgtggcgc cgtcgagcgc gacgaaatcc
    28561 gtcgggtggg ccggcgcttt tcgtttccgc gccttgtccc agagccgcag cgccaggggc
    28621 agcgccagcc agacgagcag cgccggccag ggcatgccgc aggcggcgtc cggccgggcg
    28681 atgccgaccg cggccagcgc cgtgacgatg atgaacggcc cgaagacaag cgcgccgtag
    28741 tagagcagtg agccgcgatc acccagcagc gaggccacgg tgcggatgcc cgcccgctcg
    28801 cggtccgtgg cgatatcccg ccagttgttc gcgtgcagaa tgccgatcac cagcagggac
    28861 atgggcaccg cccatagggc cggccgccag gagggctggc ccgtttgcac cgtccaggcg
    28921 cccagggcgc cgaggatgcc gaagtcgagg aagacggcca ggtcgcccag cgcgcggtac
    28981 ttgagggccg gcccgagagt gtatccaatg ccgatcgcga cgccgatcac gccgatccag
    29041 aacagaggcc atccgatgcg gagggcgagc ccgaggccga tggccgagcc ggcgaccagc
    29101 agcaggatcg cgccgcgccg gacctgcgcg tcggacagca agccccgcac gatggagccg
    29161 ctggcgggcg tggcctcgcg atcgaggcct ttgcggaaat cggtcacatc gctcaacatg
    29221 ttggccccgg tgtggagcag gaccatggcc agcagggcgg cggcaaacaa cccggggtgg
    29281 aacgccgccc cgccttgcgt cgcggccgcc gtcgtgccga aaacgacggg catgatggag
    29341 gccggcagcg cgaaggttcg cgccgcaacg agccacttgc gggcgggatg catctcgggt
    29401 ggcatgccct atgcataacc cctttttccc gaaaagcaac agccatcacc catgctcaca
    29461 cttctaaaag tgtgagcatg gatggaatag tagcaaccta tttataaata gcagataaca
    29521 agtaatatca ttaatactgt attttctgat catgatcaca tatttagaag tgtctggcgg
    29581 ggaacgggca tctcggcttc cggttgagcg ggatgaagcg tgcgccgatg gcgcgggccc
    29641 gctgcaaaaa aaatgcgcgc cggatcagcc tggtgtacga tgaggccatg caggatccac
    29701 agcagtttca catggatgtg agccgcttgc cggcggagag ggaatggcgc ggcgcgcgct
    29761 cgctggggat attcctcatg ctgttctccc tcgtgtgggg agggctcccc gccgccatgc
    29821 tggtgctgac cctggcccgg gagggcttca gcccggccat catgccggcc ctggttttcc
    29881 ccgtcgtcgg ggccgcgatg ttcctggtcg gcttgaacca aatcgtcctc cgcggccggg
    29941 ttcgtatcga ggcgtccgcg gttttcttcg agcgccgaag tctgttcaag cgatcggaat
    30001 ggaccgagcc gctggcccgg tacgcggggc tcctctacag gacggaacat cattccgggg
    30061 ggaagaaccg gccttcctat acgctgtata tcgtggaact ccatcacccg gaaaaagccc
    30121 ggcgggtgac gatctgccag tccaagtcgc ccgacggcat gcgggccgcc tgggagaatc
    30181 actgccgggc gctgaacatg gccgcgctcg aggagtcggg cgggcagttg atccggcgcg
    30241 ggccggcgga cctggacaag tccgtgcgcg acctcgcccg ggaacgcaag ctggacctgg
    30301 cgttcgatcc gcgtcaagcg ccgccccagg ggatccaact cgagatcggc cgcgatgaac
    30361 tgcgcctcgc catcggcgtg ccggccctcc cgctgaaagt ggcggtccca tgggcactgg
    30421 ccgcgctggc gatgatcggc ctgccttttg ttgtcagggc tgcgccgttg ttcgtggcga
    30481 cgctgggcct gatatcgctg gccgtgccgg tggcctggcg cgtgttgtcc ccccggttcc
    30541 ggcaggtcct ggtggtctcc cgcgccgagg tgcgggtggg ctgggaaacg ccatgggggc
    30601 ccccggcgga aaccgtcctg ccggccggcg agatcgaaca ggtggaggtc cggcggggag
    30661 cggggggcca gtccccgacg ttgcatatca tcaccgaccg gcgcgaggtc tcggtcggcc
    30721 cgttcctcaa gccggccgac cgcgactggc ttcgccactg catcctggcc gtgatttcgg
    30781 ccggctagca aaaccttcag ccggccggcg cggccgccgt gaccgggtgt tccgcgtccg
    30841 ccggctgggc cggttcagcg ttcatggaga tcaggtcgct gaggatctgg agcgcctcgt
    30901 tcagcatgac gtcggcctgc tggtcgccgc cttcatcctt ctcgcgcaac tggtcgctca
    30961 actctttttc cgtgcgggcc agttccaggc gcttttcgaa gttcagcgtg accgtcggat
    31021 ccttctgccg ctcggcgagc cggccgagca gttccaggta ggcctggtag cgggcgtcgg
    31081 acttgcgccg ggcctccacc ttgctccgca gcgcgggcat ctgttccctc aagggcgtcg
    31141 ccagggcgta cgtcagggac cggatctggg accagggcag ggcctgcggc agcgtctcct
    31201 cgccgatctc cagggtgtcg aggctggacg gcgtcacgat gtccggggcg acgcctcgca
    31261 actgcgtcga gcccccgttg atccggtaga aactggccgt cgtgaccttc agcttgccga
    31321 gcgccggctg cccgtccttg aggtcggcga gcgtctggac cgtgcccttg ccgtgcgtct
    31381 tggaatcgcc cgcgatgacg gcgcgcccgt agtcctggag cgcgcccgcc aggatctccg
    31441 aggcggaggc cgtctgccgg ttgaccagca cgatcatcgg gccgtcgtac acgacttccg
    31501 ggtcggaatc gttcatgacc tggctccgcc gcccgtcgct gacctggacc acggggccgc
    31561 gctcgatgaa gaggccggtc atccgcacgg cctccgccag caggccgccg ccgttgttgc
    31621 gcaggtccag cacgagcccc tcgacgccgt tggtggacag ctcgcgcagc agccggcgca
    31681 cgtcctgggc cgagctgcgg gcctccgcgc ccagcccgcg cagcggccgc acgtcggcgt
    31741 aaaagtcggg cagcgtgatg atccccagct tgcgggttcg gccgtcggcg ccgaccacct
    31801 ccttgaccgc cgacttggcg gcctggtcct cgagcttcac ctcttcgcgg atcaggtcga
    31861 tcttgacgac cgtggtcccc gtggggtcgg aggccgggat cacggacagc acgaccttcg
    31921 tattcttctc cccgcggatc aggcggaccg ccttgctcaa cggccagtgc aggatgctca
    31981 cgggctcccc gtcgccctgg gcgacggcga tgatcttgtc gccggccttc aggcgcccgt
    32041 ccttctcggc ggggccgccg gggatgagct ggtcgatctt cgcggcgccg tcctcgctgg
    32101 tcaggcgggc gccgatgccc acgagcgaga gctgcatgcc gatctcgaaa tcctccgtct
    32161 ggctggcgga catgtagtcc gtgtgcgggt cgtacgcctg ggtaaaagcg gtcaggtagc
    32221 ggtccaccgg ccactcggcc cggttgtcgt tgagcacgtc aaggtactgc cggtagctct
    32281 tccggatggc ctcttccggc gtgaggcgca ggtcggtgga caaatccacc tcttccgacg
    32341 gttcgaccgc cggctcctcc gggagggacg cggccggctc gttggtgcgg gcggcgtccg
    32401 ccatccggcg gttgaccatg tgcgcgatgt actggttctt gatcttccgg cgccagaggt
    32461 cgtcccaggc cttctcgtcc gcgggccact cggcgtcctt ccgcttccac gtgtacgact
    32521 cgtcgcggtc cagttcgaag ccctgcttca gcaggccctc cacgaagtcc acccggttgc
    32581 ttacccgcgt ccggagggtg tcgtatgctt cctgggcaaa ggagacgtcg cccgcgatca
    32641 gccggttgtc caggttggtg gcggcgagct ggaaccgctg aatgtccgag gccaggaagt
    32701 agctccggtc gaagtccagg aacgccagga accggtccag ggcggcgacc gcgcggtcat
    32761 cgtccatcgg caactgggtc aggtgccact ggggcaggcg ccgggcgacg agtcgagccg
    32821 cctcggaagc ctccgcctcc ggcgcgctcg ccggcgcgcg ggccgcggcc cagaccatgc
    32881 cgccgaccat cagggcgcaa ggccccagcc atcttttcca gcctttcatc aaattgatct
    32941 ccgccgcgta ctatagggta aactccgcat cttttctaca ggactcttgc cggccgcgcg
    33001 ccgcatgacc gggccataga cgccggggcc gcgccccgtg ttcatttttc ccggcgccgc
    33061 gcaaagcagg cttggtttcc ggccggcccg ctgtagtatg aaatcgtgaa ccggttcgaa
    33121 ttgagaaggg ccgccggcgt ggccgggctg ctggtgctcg gcgccgggtt cgtctttttg
    33181 ctgttcatcg ggtcctcgct aggctggttc gagcgcgagc cctcggcgcc ggcccgcgcc
    33241 cggccgggcg gaacccccgc ggccttgtcc gcggcgccgg ccgcggcggc gcccgccgcg
    33301 ccgatcgccg ccccctcgcg acgggccggc ccgtccgggg gcggccccgg ggccctgaag
    33361 gacgcggagg ccctggaaac ccgtttgcgg gagatcgaga cggagaaccg ccggctccgc
    33421 ggcgaggtcc aggacctgca cggccggctc gtgggcgtgc tgaactgggt gctgacgaac
    33481 ttcaggggca cctacccgct gcccgagccg ctgatggccc ggctccaggt gccgcccgtg
    33541 acggaggatt acaccctgca tcccgaggcc gccgcgctga tcaaggtcac gccggaagag
    33601 gagcagaaga tcaacgatgt cttcgcgtac gcgcgctcct acctcgccga gatcgaggcg
    33661 gccatgctga ccgtcaccag cccgcgcccg gacaaggtca tcctgcacat cccgccgttc
    33721 gcggaggacg gggccgcgct gcgggaggac atgtacgcgg ccctggaaat cacgctgggg
    33781 gcggaccgct tcgaccgttt cctcaaggtt tccgaggcgg ggctcaagtc gagcttctac
    33841 cgtttcggcg aggcgtcccg gacgctcgtg ttcgagctcg tctacgcggg cgagggggag
    33901 ccgccgcagc tgaagatcaa ggacggctgg atcatggaga ccgtgccggg cgccaaggag
    33961 atcacggcca cggaggccac cgtcacgaat ctgccgtcgc ggtacgccgc gtattcgccc
    34021 tggctgcccg actacctcgt gcagggattc gctaccgagg ctgttcggta ggcggcgggc
    34081 ccttccactt cgccatttcc ttctggtagc aggccgggca gatcccgtgg ctcaccttgt
    34141 tcatctcgcc gggcttgacg tcgatccact tgccgcaggc ggcgcacttc acgtacatca
    34201 gctcggcgga ttccttcgcg gtcttttgtt cgtggcggct cacggcgccc tttcatcggc
    34261 tcggccgcgc agggcggcga ccccgttgtt gtgacaggaa aaacgggccg gggcaagtct
    34321 tctcgccggc ccggggcgcg gctgcttttt ccgcttgaaa acagggtcgt cgcggtggag
    34381 ggtacccgcc atgcggcgga acatcatcga cgtgcatacc cacgtgttcc cggacgacct
    34441 ggccgcgcgc gcgatccagc acctgtccgg taattccggg gagcgggcct tcacggacgg
    34501 caccgcgggc ggcctccagg cctccatgcg ccggcacggc atcgccttga gcgtcaccca
    34561 gccggtctcc acccggccgg cccagacgcc cagcatcaac acctatgtca tggaattgaa
    34621 gcagccgggc ctgatccact tcggcacgct gcacccggac tacgggaacc cggacgcgga
    34681 gatcgagcgc ctggcggccg gggggatcaa gggcatcaag ttccacccgg actaccagaa
    34741 cttcttcgtg gacgaggagc ggatgttccc ggtctacggg aggatggccc gggccggcct
    34801 gatcgcgttc ttccatgccg gggtggacat cggcctcccg ccgcccgttc attgcccgcc
    34861 ggaccggctg gcccgcgtcc tggagcgcgt gccggacctg cccgtcgtcg ccgcgcactt
    34921 cggcggcttc aagatgtggg acgaggtggc ccgccatctc gtcggcaagc cgctctggct
    34981 ggaatcctcg tttacattgt cgtggctgcc cgcggcggat ttcacgcgga tggcgcggcg
    35041 gcacgggatc gagcgggtga tgttcgggac cgactcgccg tgggccgacc agggcgcgga
    35101 gatcgccctg atggaaaaga ccgggctgaa cgaggccgag ctcgacgccg tcttccacgg
    35161 cagcgccgcg cggctgctcg gcttgtaagg cgcggcgcct ccagccctcg aaacgcgtct
    35221 ctcacgggcg cgcggctttg ttcaggctct tttcccttac ccagctgtac aggaccggcg
    35281 ccatgaacat ggtgacgacc tccagcagca tgccgccgaa ggaggcgagc gacatgggca
    35341 ccatgagatc cgagccccgg ccgcgggacg tgatgaccgg cagcagggcc agcaccgtcg
    35401 tcgccgtggt catcaggcag gcgcggatcc ggcgcccgcc ggcgaagacc gtggcctcgc
    35461 ggatcgcctc gcgggtcgcc ggccggactt cctcgaaccg ctgcttcagg tatgcgcaca
    35521 tcaccacggc gtcgtccgag gcgatgccga acagcgccag gaatcccacc cagatcgcga
    35581 tgctcatgtt gacggggcgg acctggaaaa ggtcgcgcag gttcaacccg aacacggaga
    35641 agtcgaggaa ccacggctgg ccgtagagcc agatcagcag gagccccgaa ccccagcaga
    35701 cgaagatggt cgagaacacg agccccgtcg tgaccacgga ccggaactgg aagtagatga
    35761 tcaggaagat gacaaacagg gtcaggggga ggatcagcga cagcgttttc tgcgcgcgga
    35821 gctggttctc gtagttgccc gcgaaccggt acgaaacccc gggcggcagc gccaggtcgc
    35881 cggaggcgag ccgatccgcg agataggcct ggcaggcctc gaccacgtcc acctcggcga
    35941 agccgggctt cctgtcgaag aggacgtagc tgacaaggaa gccgtcctcg ctcttgacgg
    36001 cctcgggccc gcgcgcgtag cggatgtccg caagctgcgc gagggggatc ggggcgccgt
    36061 ccatggccgg gatcaggatg ttttgcaggg cctcgggcga gtcgcgccgc tcgcgcaggt
    36121 agcgcgcgcg cacgggatag cggtcgcggc cctcgacggt cgtcgtcagc cggacgccgc
    36181 cgacggcgac ctcgatggcc atctgcacgt cctcgatctt catcccgtag cgggcgagcg
    36241 cctcgcgccg gagctcgatc tccaggtacg gcttgccgac gatccggtcg gcgatcacgg
    36301 cggagggctc gacgccgggc gcctccttga ggaagcgctc gatatcgagc ccggcccgtt
    36361 cgatggcctc caggctgggc ccgctgactt tgacgcccat cggcgcccgc aggccgctct
    36421 gcaacatgac caggcgcgcg gcgatcggct ggagacgcgg cgcgctcgtg ctgccgggca
    36481 cctcggccgc ccggacgatc tcgtcccaga tgtcgtcggg cgtgcggatg tggtcgcgcc
    36541 actggcggta gggcttcccg cgcgggtcgg ggacgaggcg gccgtccgcg tcgcgcgcga
    36601 attggcgttg ctggcggtcg taccgataga ggagcgcgcg cccggcctgg tccgtggcgt
    36661 actcggattt gtaggcgatg accgtctcga tcatgttcac cggcgccgga tccagcgcgg
    36721 acgccgcgcg cccgatcttg cccacgaccg actccacctc ggggatcgcc cgcagggcca
    36781 tgtcctgctt gcggacgatg tccatggcct cgccgaggga ggcgtgcggc atggtggtcg
    36841 gcatgtacag gaaagacccc tcgtccagcg gcggcatgaa ttccttgccg aagccgggaa
    36901 aggcgtggac cgcccacgac cacggaccgc tctggcgcag ggcccgggcg gcgggccagg
    36961 ccttttcgag gccggcgggc acgaagccga aaagccggtc gaacccgagc caggccgtgt
    37021 acccggcgat cacgatggcg gccggcagcg agaggaacgc gagcttgtgc tccaggcacc
    37081 accgcaggat gcgggcgtag cggcgttcga ccaggtagta gaacccctgg agcgcgccga
    37141 tgacgacggc cacgaacacg aagttccgca gccagccctt ctccggcccg agcggcagcc
    37201 agtggcccgc cagcagcgcc ccgacgagga gcaccgcgag ggcgttggcc agcccgggcg
    37261 ctacccggcg gagacgttcg ggcaggcgct cctcgacggc cacgtaaccg ccccacagcg
    37321 cgaagagcgg cgccgcccgc caccagccga acagcacgcc catcgccacc cccgccagga
    37381 ccagccccgc gcccagcgcc tgctggatcc ggttcgacgg cggccgccgg cccaggagaa
    37441 gatgcgcggc cgccggcacg attgtcagcg cgatgaccac ggagccgacc aggcagaagg
    37501 tcttggtaaa ggcgaggggc cggaacagtt tcccctccgg gccggacatc gcgaagacgg
    37561 gcaggaaacc cacgacggtg gtcgagatgg cggtcaggat ggcgccgccc acctcgccgg
    37621 cggcgcggta gatgacctcc agccggctct cgtcgcgccg cgcgtcggcg aggtgctgca
    37681 ggatgttctg gcaggtgatg atgcccatgt ccacgatcgt accgatggcg atggcgatcc
    37741 cggacagcga cacgaggttg gcgtccacgc cgaacagccg catggcgatg aaggaaaacg
    37801 cgaccgccag cggcatcacc gcgctcacga gcagcgagag cttgagctgc gccgccatga
    37861 cgatcaccac gatggtcgtg atgaggatct gctcgtagag cgccgcgttc agggtcccga
    37921 gcgtctcgta gatcaggccc gtccggtcat agaacggcac cacggtcacg cggctggtgg
    37981 tgatccccgc aggccagcgc tcccgcggaa cggcgttcag ccgcgcgagc cacgcctcct
    38041 ggtccagcgc gccgtcacgg aacgcctcga agccttcccc gcgcgcccac tgctccaggt
    38101 ccgccttcgt ggcgcgccgg taatcggcga cggccttgac cggcagggtg ggggcgatct
    38161 cggcgatccg ggccttgacg ttccggatgg cgtccagggg gttgtgcccg tggcggacca
    38221 cgacgacccc gccgacggcc tcggcgccct ccttgtccag cgcgccgtcg cggaacgcgg
    38281 gcccgagcgc caccttcgcg acgttccgca cgtagatcgg gacgttgccg ttgaccttga
    38341 cgacggtgtc ttcgaggtcc ttgatccctc ggaggaaccc gcgggcccgg atgagatact
    38401 ccacgcggtt cagctcgatc tggcgcgcgc cgacgtccag gttcgacgcg cggaccgccc
    38461 ggaagacctc ttccagcgac acgttgtgaa tccgcagcgc gtcgggatcc acgtccacct
    38521 ggtactcgcg gaggaacccg ccgatcccgg cgacttcggt gacgcccccg gccgcctgca
    38581 ggtggtagcg gacggtccag tcctggatcg agcgcaactc gtcgagatcc cagccgccgg
    38641 ccggattccc gtccgggtcg cggccttcca gggtatacca gaagacctgg cccagcgccg
    38701 tggcgtacgg accgagcgcc ggcttcacgc ccgcgggcag cgtgccctcg ggcaggccgt
    38761 tgagccgcgc gatcacccgg ttctgcgcgg cctcgaacgg gacgccctcc tcgaagatca
    38821 cgtagaccat ggagaagccg aacatggagt agctgcggac ggtcctgacg ccgggcatgc
    38881 ccagcagcga gacggtcagc gggtaggtga cctggtcgtc cacgtccttc ggcgagcggc
    38941 ccatccagtc ggtaaagacg atctgctggt tctcgccgat atccgggatg gcgtccacgg
    39001 gcaccgggtg gcgcacgagg cccgtgtgcg cccagtcgaa aggcgcgacg atgacgcccc
    39061 agatcgcgat gaaggcgacc aggaggccga cgaccagttt ctgctcgagg cagaagcgga
    39121 tcacgcggtg gaccgggccg gccgggcgct cgtcgtcagt gggcatgcgg gcctcccggg
    39181 agaggttcgg gctccacgcc cgcggcgcgc atggcctcga ggtacttctc gggtccggcg
    39241 gaaaaggcgg cgtcgcaagc gccgcagcag aagtagatcc gcaggccctg gtaatcggta
    39301 tagaacttct tatcaatctt gccgcccatc acggggcaca cgctctgggc tttcgactcg
    39361 gcggcgagcg ccgccggggg gctcatcatg ctgggccggc cctggatctg gagcgaactg
    39421 tccagtttga aggcgccgct cgtcaccacc cgctcgccct cctcgaggcc ggaccggacg
    39481 acgtagaaat cgccggcccg cggccccagc gcgatctcgc ggccctcgaa cgcgccctcc
    39541 tcgccgggga ccgcgacgta cacgacggcg cgcgcgccgg tgatcagcgg cgcggacgcg
    39601 gggatcacca gcggcgccgc cccggtcccg tcgcccgccg tggacgcctt gaccgttgcg
    39661 cggatgaaca tccccggctt cagccggccg ccgggattcg gcacatcggc gcgcacccgc
    39721 acggtccggg tcatcgggtc gaccaccggc tcgatgaacg cgatcgtgcc gcgcaagggc
    39781 tcgccgggat aagcctcggc ctcgagtgcc gtctcctggc cctcgcgcag ccaggcgagg
    39841 tccgactcgt aggcgtccag cacggcccag accgtcgaca ggtccgccac cgtgaagagc
    39901 ggcatgccgg cctcgacata ctggccctcg cgggcgtcct tctccaccac gacgccgccg
    39961 agaggggcgt ggaaggcgag gtgctcccgg acctgccccc cgcgttcgat ctccgccacc
    40021 tgctcggacg tgagccccca gagccgcagc cgctcccggg cggcgtcgag gagcgcgccg
    40081 gcccggccag cctgcaggag ttcctgctgg gcggcgagca gttcggggct gtacaagtcc
    40141 gccaggggat cgccggcctt caccgcgatg ccggtgaaat tggcgtaaag gcggtcaatc
    40201 cgtcctccga cccgcggcgc aatggttgcg acgcgcgtct cgtccggttc gatccggccg
    40261 gcgagccgga cgtccaccgc cacggggcgc cgctccgcgg gtgtgacgtc tacctccgcc
    40321 aggcggcgcg ccgccgggga aagcgccagc cggcgcggcg gctcatcccc gccgcgggaa
    40381 gacgcggccg ccgggaccag gtccatgccg cacagggggc acaggccggg cccgggctgg
    40441 cggatctgcg ggtgcatcga acaggtccag atggccgccg tctccgcgcc gccggcggcc
    40501 gcgctcccgg gcgcgccgcg ccgggggccc gcccggcccg cgaaaaaccc gaacgccagc
    40561 agaaggattc ccgccgcgag gtatctcttt ttcaccgtgc tccgcctccc gccggtccgg
    40621 gcccggcgcg cgggggcgcg gccaggacgc tttccaggtc cgccccgtat ttcccgatgc
    40681 agcagccgat ctcgaccagg gccagctccc gctccacgag ggcccgctcg tgcgccagcc
    40741 ggaactcgag gagcatccgc tgcacgtcgt tgacgtccat gaactcggcc cgcccggccg
    40801 cgaacgcctg ccgggccacc gcgtaggcct cttccgtccg ggggatcagc gaatcccgga
    40861 acagcgcgat ccggcgctcg ctgtcgcgga ccttgaacac cgccatgcgc agttcggtct
    40921 tgagctcgtt gaagcggtcg gcctgctcgt tccgggccga ttcggccatc gcggcggcct
    40981 cgcgcctccc ggcggagttt ttgccccgcc accaaggcaa cgtgatcccg accatcaccg
    41041 acgcgtcgta ttcgctcccg ccgccctcca tggacggcat gctcatgacg ccggcgccca
    41101 gcatcaggtc cgggcggccg cgccgccggg cgagttcctg cgtcgcccgc gcccggtcgg
    41161 tcatggcctg cgcggccttc agttccgggt tcaaatcctc gatcatggcg aggagcgccg
    41221 gctcgtccag gagcgcggag gcggacggct cgacggccgg ccaggggagg ggcgggccgg
    41281 cgggccggtc cagcagggcg gagagccgat ccgacaggac cccgcgctcg tcgcgcatcg
    41341 aggccagttc gctggcggtc cggtctttct ccacctgcgc tttcgtgaga tccgggaagc
    41401 cggcggagcc ggcttcgtag cgggaccgga tcacgttctc caggtccgcg agcaagttgt
    41461 actgctcctc catgacggcc agggaacgcc cgagcaggtg ataatcgtag aacgcgcggc
    41521 tgacccggtc gtagaggtcc agccgggccg cctcgtattc gtgccgggcg gcccgggctt
    41581 ccgcggatgc ctcccgggcc atccagccgc gtcgcccgaa cagggggatc atctgggtca
    41641 gcgccgcctg ccagcgcacg tccttctgct cgaggaaata ctcgtaggac agttgcggat
    41701 catccagggc cttcgcctgg gggacgcgct cgtccgcggc cgcccacttc tggcgcatcg
    41761 ccttcaggcc ggggttgtcc cggaacgcgc gctgaaggat cgcgtccagc gtggcctgtt
    41821 catccggccg ttccccgccc cgggcggcgg gcccctcgag ggccagcagg gcgagggcga
    41881 gggccccggc gattccgaac gggcggcggg gggtcatggg tcagcctccg cactgccgga
    41941 cgaccttcag gatctcggcg atcttggtct cgatatcgcg tttcgagcgg ctccggaacg
    42001 cgtcggcgac gcagtgttcc atgtgttttt tcaggacccg gcggccgacc gcctggaggg
    42061 cggcgaccga ggcctggacc tgggtgacta tgtccatgca gtactcgccc tcctcgatca
    42121 tcctccggat cccgcggacc tgtccctcga tccgggcgag ccgcccgcgg ttctcgtcgt
    42181 gtcgcgtctt gtggtttttc atcggttgct cccgagcccc actatacccc ggtggggtat
    42241 attatcaagg cgcgcggccg gggcgccgcg ccccgcggcg cccggccggt ccggcgcggc
    42301 gaaatgcggc ttgagcgcgg gggccgccgg gggtatcagt aggaccggtg tggcggaggt
    42361 gcccggcggc ggggagaggg acgtatgacc cgtaatattc tagtccagtg ggcccagata
    42421 caacgcaagc tgaccggatt gtccgaccac accctcgagc cccacggggc cgggacctgg
    42481 tcgcccaaca cggacgtgta cgaaagcgcg gacgacgtgg tcatcaagat ggaactggcc
    42541 ggggtgcccg cggagagcgt gcatatcgtt ctcgaaaacc gggtggtgcg cgtggagggg
    42601 ctccgccgcg acccgcagag cggggagagc gccgccggat accgcttccg ccagatggag
    42661 atcgagtacg gggtgttcag ccgaacgatc gagctcccgt tcgcggtgga cggcggacag
    42721 gcgcgcgccc ggatgcagaa cggcgtgctg tccgtccggc tgccccgcgc gcgggaggcg
    42781 gacgggcacc ggatcaccgt gaccacggaa aatcagccat gagcgatgcg cccgaatcca
    42841 gttcgcctcc tccgggaccg gcggatcccc ccccggcgaa cggggagcgt ccgagcccga
    42901 gcccgatccc tcccgttctc cccgtgctgc cgatgagcga cctggtcctg ttcccgtcca
    42961 tggtcgcccc gctcgtggtg aatacgccca agtccgcccg gctcatcgac gccgtgaccg
    43021 cgggcccccg cctcctgatc accgtgctcc agaaaagccg cgcggcgtcg gacgatgccg
    43081 tgggattcgc ggacctgcac gagtacggtt gcgtggcgcg gctgatcaag atgctcaagt
    43141 tccccgacga aacggtgcgg atcctcgtgc aggggatctc ccgctgccgc ctggcgcggc
    43201 cccatggcaa cccgaatttc ctggaggtct accacgcgat ccttaaggac gaggtcgagg
    43261 actcggtcga gaccatggcc ctggcgcgca acgcctccca aaagttccag gagatcatca
    43321 ccctctcgcc cacgctgccc gaggaactga agatcgccgt gttcaacgtc gacggccccg
    43381 gcgcgctgac ggacctgatc gcgtcgcacc tgaacatgtc gctggaagac cgccaggcgc
    43441 tgctggagga atatcgcgtc aagggccgcc tgaaccggct caccacgctg ctgaatcgcg
    43501 agttcgaggt gctgcgcatg ggcaccgaga tccagcacaa ggtcagcgag aacttcgcca
    43561 agacccagcg cgagttcttc ctccgcgagc agatgaaggc catccgccag gaactggggg
    43621 agaaggacca gcagcagatg gaaggccagg agatcgagaa gaagatcgcg gccgctcggc
    43681 tgccgcccga ggccctcgag gccgccaaca aggagcgtga gcgcctgctg accatgccga
    43741 gcgcgtcgcc ggaatacgcc atcatccgca cctacctcga ctggctgacg gaactgccct
    43801 gggcggtggc caccgaggac gcgctcgaca tcgcccgggc gcgcgcgatt ctcgaccgcg
    43861 atcattacga cctggccaag gtcaaggacc gcatcctgga gtacctgtcg gtgctcaagc
    43921 tgaagaagga cctgaagggg ccgatcctgt gcttcgtcgg cccgccgggg gtgggaaaga
    43981 cctcgctcgg gcaatccatc gcccgggcgc tcggccgcaa gttcgtgcgc atgtcgctgg
    44041 gcggcatgcg ggacgaggcg gaaatccgcg ggcaccgccg gacctatgtc ggcgcgctcc
    44101 ccggccgcat catccagggc ctgcggaagg ccggatcgcg caacccggtc ttcatgctgg
    44161 acgagatcga caagctgggc caggacttcc gcggcgaccc gtccgcggcg ctgctggagg
    44221 tgctggaccc cgagcagaac acggccttca cggaccatta tctcaacgtg ccgttcgacc
    44281 tgtcctcggt gctgttcatt acgacggcga acctcgtgga ccccctgccg cctgccctgc
    44341 acgaccgcat ggagacgctc gaactgcccg gctacaccgc caacgagaaa gttcatatcg
    44401 ccggccggta cctggtcccg aagcagatcg ccgcgcacgg gctcaagcgg tcgcagctgg
    44461 cgttcccccg ggccacgctg cagcgcatca tcggcgatta cacccgcgag gccggggtgc
    44521 ggaatctcga gcgccagatt gcgtcgattt gccgcaagac ggcccggcgc gtggccgagg
    44581 ggcggcgtgg cgcgaagcgg gtgaccccgg aggccctgca tgggttgctc gggccgaagt
    44641 tgttcgaggc ggaggtggcg gagcggcagt tggagcccgg gatcgcgacc ggcctcgcgt
    44701 ggacctgggc cggcggcgac attctcttca tcgaagcaac gcgcatgccg ggccgcgggc
    44761 tcctgacgct gacggggtcg ctcggcgagg tcatgaagga atccgcgcag gcggcgttga
    44821 gctatgtccg ggccaacgcg cggaagttgg ggctgccggc cgatgtgctg aaaagcaccg
    44881 acattcatat tcatgtcccc tcgggggcga tcccgaagga tggcccgtcg gccggcatcg
    44941 cgatgctctc ggccctcgtg tcgctgcttt ccagccggcc cgtgcgggcg ggcgtggcca
    45001 tgacgggcga gatcaccctg cgcgggaagg tcatgcccgt cggcgggatc aaggaaaagg
    45061 tcctcgcggc ggcccgggcg gggatccgga agatcatcct gcccgcccgg aaccgccgga
    45121 gcctggagga cgtgccgtcc gaagtgcgcc ggcgagtgaa attcgtgctt gtggacaatg
    45181 ccgattcggc cctgcgggcg gtactggggc cggggcgaaa ggcggggacg tgaaccgggg
    45241 cgtcgtgtcg gctgtgctgg tggtgtgggc cggcgccttc tcgtgggcgg cgatggacga
    45301 ggagcccctg ctgcgccccg atgcgtcctg gccggacgcc gtcgaactgg tccaaggcgt
    45361 ggtcgccggc ctgccggatg tgccggtgcg gatggaagcg cagttgcagt cgaaggaccg
    45421 tcgcggaacg atcgagggca cgctgaacgc ggaaatgaag ctcgattggt acggccagcc
    45481 gccgagcgcc gagtaccgga tccgcgacgc ctttggggcc gagcgcgagg cgctccgtct
    45541 cgactggagc gagcccggcc gtcccgtcta ccgatacagc cgcggcgaac cgccggcgag
    45601 cgagccgctg ccggatttat accgcccgat cgaaggcacg gacatcagct ggatcgactt
    45661 gagcttgtcg ttcctctggt ggccgggcgg ccgcaccgtc ggcatggaaa agatcaaggg
    45721 gcggatgtgc tatattgttg aaatgcccgc tccggcgggc gaggcgggcg gatacgccgg
    45781 ggtccggctt tggatcgagc cgcgcatccg gatgatgctg caggcggcgg cttttgacgc
    45841 cggcggcgag gtgctgcgct tggtcgaggt gaaaagtttc aaaaaggttc gcgacctgtg
    45901 gctgatccag aacctggatg tgcagacgtt ccccgggcgg catcgaacga gcctgcgggt
    45961 tcggcacgtg gcggtggctg gcgaggccat cgaggagggc gggaaaggcg gcggcgagga
    46021 cagttaagga gcgcgacgtg gccgatatcc aattgaagtg cgcccagtgt ggaaaggaga
    46081 tcaccgtctc cgagttcgtg tccacggagg cgttgacctg cccggcctgc ggcgcggccc
    46141 tgtccatgcc ggaacggaag gcggaggccg tgcctctgaa gatccgcctc caggagcggg
    46201 agtcccggac cgcgctgacg ggccatgaga tggacgtgga tgttctggcc cgggccgtag
    46261 cggccaaaga gacggactcg gtcatgggcg aggtgcacaa ggcccggacg caggtcaaga
    46321 agtccaccgg gatatggctc tggatcattc tcctgctggt cggcggcggc ctcgtcggat
    46381 ggcagcatgc tgtggcggaa ggaatcgggc ccgccggact ggcggacagt tacggcatgg
    46441 cgcgggcggg catcgccggc ctggccgcag tcctcgtcct ggccgtggcg ttttacgaga
    46501 gctgggtcca gggcgtgctg tgcctcttgc tcccctttta tatcctgtac tacacgattg
    46561 ttcgcatgga gttcaacacg gtgcgcggct tgttccttgg cgtctgcgtc gcgatcgccg
    46621 tcgaggtaag gctgcttccg gatcaatcgc tgctgttggc ggcccagggt cgcttcgatc
    46681 agtttgtcac cgccgtgaat cagaagatca agcgagccgg ggagccgccg agcatgccgc
    46741 cgagccgaag gcgtggcagg catgctaaac cgaagtcctt taatgctgtc ccggtcttct
    46801 ttgacgttgg cacatgctcg cgccggcaag gagactcccg ggagcttctt cttcctgcct
    46861 gattgatggc aacaaccctg cgcgaccgta tggcgtgttg gccggcgcga cgcgaacgaa
    46921 aggcctaatt cctggcactg cttgttcgga cggacttcag ttcggtcgtt catgcattct
    46981 tgtgcattgc atatcacact ttgcaaagtg tggaagtgaa atgagtaatt acattcttgt
    47041 aattaagtag ttatataaat aacggtattc cagcttattt ccacactttg caaagtgtgg
    47101 aagtagcggc atcggtttat ttgcggcaag cggtttttca ggcgctggaa ttaacaaaag
    47161 aggccgggtc gtggtcgggt gcactgcagg gcgggccgcc ccgtcggtct ctcgcgggcc
    47221 tgaatggggg atcactccat catcgacagc gtcgccgcgc cgatgacgcc ggcgtcgttg
    47281 cccaactccg cgggcttgac cacgatccgc tccgcgaaca ccgggctcaa ccgctcggtc
    47341 aggtgccggc gcagcgggtc gaacaggatc gccccgctct gggcgatgcc gcctccgatg
    47401 atgaacgcct gcggctgaat gaggtaggca accgacgcga aggccgccgc caggcaatcg
    47461 gccatgaagt cgaaaagctc cagcgccagc gcatcgccct ggccagccgc cttcgcgatc
    47521 agttcgaccg tgatcgccga gaggtcgccc tgcgccagtt cgcggatcag cgatgcgcgg
    47581 ccctgctgga gggcctgaac gacgcgctcc accaggcgcc ggttgccgac atactgctcc
    47641 aggccgccgc ggccctgcgg agacttgacc ccgtccatcc ggatcgggac gtgcccgatc
    47701 tcgccggcca tcgagtaggc gccgcggtac aactggttgt tgatgacgat gccgccgccg
    47761 acgcccgtgc ccaaggtgac gaacacggcg tgctgatagg cgcggcccgc cccgtacgtg
    47821 cactcgccca gggccatcac gttgacgtcg ttatccaccc gcacgtgcag atggaaccgc
    47881 tcctcgatcc tcggcgcgag atggacgttg gtccagcccg gcacgttggc caggtcgtaa
    47941 atgaaacctc gctcgaaatc cacgaaaccg ggcaccccga cgccaatacc ggccaacggc
    48001 gcgtccgcag gggccttctt gtccttcaac tgctccagtc cccggcccac ggcgtcgagc
    48061 cagccgtcga ggccgggcgc atccttcgtc gggatcttcg cgcgggcctt gatctcgccg
    48121 cgctcgctga cgagggccat cttgatgaag gtcccgccga aatccacgcc gatggcgtgg
    48181 cggatgctgg tggcgtcttt catgatctcg ctccgaggaa aagcctctcc gcgtttgccg
    48241 tggtactatg ctcgatgagt tcgaggctgt catttcgaat tttcgcgagc acctcggcca
    48301 cgggcacgac ccaggccggc tcgttgcgct tcccgcgatg aggcacaggc gctaggaatg
    48361 gcgtgtcggt ctcgatcagc aggcggtccc ccgggatgcc ccgagccact tcgcgcagcg
    48421 gatcggcgtt acggaaggtg aggatgccac tgaagctgat catcaggccc aggtcgagga
    48481 gccgggccgc gaattccgcg ctgccggtga agcaatgcag cacgccgacc cgctccggat
    48541 cgccgggcca ggcccgggca tggtcgccca aaagcgccag cgtgtcggcg tccgcctccc
    48601 ggctgtgcac gatcacgggt agccggcggc gtcgggccat ctccaactgc gcggccagca
    48661 gcgtcttctg ggcggcggcc gtttccggct cgtaatgata gtccagcccg atctcgccga
    48721 gcgctacgac gccaggccgg gccaggtccg cctcaagccg gggcagggag ggcgcagcgg
    48781 atgcctggtc gcgatcgaat cccagggcgg cgaacacgcg gccgggccat tctgccaagg
    48841 cctccagcgc ggcgcggttg gtgtccggcg agcctccgac ggcaaggacg cgccccaccc
    48901 gggcggcttc tgcgcggtcg agggccgcgc gggcctctgc cggatcggcg aacgcgtcga
    48961 agtgggcgtg cgtgtcgaag aacatcagga accgggagtc agggatggcg attcggccgg
    49021 cgcgtcttct tcgaggcggc ccgtttccag ccaagggagc acgacgctgt ccttccagcg
    49081 cccgcgcagc gtctgctcca gggcgtcggc ttccgtcgta cggttcagca cgcgcaggat
    49141 ccggagcgcc agggcgcccg cccgcacggc ggtatcggcc gtggcggcct gctcggcgac
    49201 cgcccggtat tcctccgcgg cctccgggaa gagatcgttg cgggcgtaca actcagccaa
    49261 tttttcgcgg aggatcgggc tcccggtttc ggccagccag cggcggcata gccccaggcc
    49321 gatgttgaaa cggccggcgc ggaccagctg gttgaccgcg cgcgcgcggg cccagtccgt
    49381 tgccggagcg ctctcttccc cgggcacgtg gcggaagggc cgatagagcg ggtccccgac
    49441 gacggtaacc tgccaggaga gcacgggctg cgccaggtag gcgctctcgc cgaacgtcag
    49501 gccgcggcac agccggtcga tgaagacgga caggtggggc gtaaggttga gcagcggctc
    49561 gcccacgcaa cccatgctgg cggaggcgcc ggccgccagg agcgggccgg tccagtgctc
    49621 ggcaggcgtg cggagctttt ccgcgctggt cgaatgaata tgataggcga ccgctcccgg
    49681 ctcgaagcgg aatcccggct gcaggaacgg ccccgtcgca ttctcgttgt accagcccag
    49741 gtacacggcg gcctgttcca tcgggaaggc gggcccccag acgggttcag ccgagtcctg
    49801 cacgacatcg agcccctcgc gcagcaggcg ctccgccgcc tccgacagcc agaaatcgcc
    49861 gagggcgtag gacccgtgag cgagcccgcg cccgtccacg taggcgcggc cttcgagccc
    49921 ccgggcctcg gccgtccggg cgtcgtcgat catccgccgc acggtggcgg cgtccggtcc
    49981 gtccagccgg gccgcgatca ggatcagggc ggcggacggc ggggcgtcgg cctcgttcat
    50041 ctggttgaac agggggttcg ggtacgggcc gcggatcggg tagtcggcgt tcagcagcag
    50101 ggccagttcg gaatccacgg cggccgcgtc cttgagatcg aaccggcgca gccggttggc
    50161 gatggcctcg ccgatccgcc agcgggtgtc ggcgatgcgc accggcacgc cgtacatgga
    50221 gaccacgtac cgtatcgagg tggacagcgt ctgccactcg ctctcgtggc ggcgcacgga
    50281 atccgaaggg cgccgcacct gttcgatcag ccggcgctcg cgcaggaacg ccagcagcgg
    50341 gtcgcggagg cggacttcgt agacctcccg catgagggtc tcgccggcgg gcaggtcgag
    50401 cgcgcacagg cggtccgcgg ggatcccccg ggcctgcatg tagtggcgcg ccagttcgac
    50461 cgattcgggg acgcggcggt tggccagcac gaccgtccgc agggcggggt cgtccgcccg
    50521 ggcggcacag gccagcagcg ccagggccaa ggaaaagagc cgggcgtcgc ggctctccag
    50581 gacgcccggc ccctttgtcc gcatggccga tccctatttc agcagcgggc tgacggcctt
    50641 caggatgggc gcgatgacca gggcgaccac gctcatcagc ttcatcagga tgttcagcga
    50701 cggcccggcg gtgtccttga agggatcgcc caccgtgtcg ccgacgacgg ccgccttgtg
    50761 cgccggcgaa cccttgccgc ccagcgcgcc ggcctcgatg tgcttcttgg cgttgtccca
    50821 ggcgccgccg gcgttggcca tgaagagcgc catcatcacg cccgtcaccg tcacgcccgc
    50881 gagcaggccg ccgagcatct ccgtgccgaa cacgaacccg atcaccaccg gcgtcagcac
    50941 gccgagcagg gcggggaccg tcatcttctt gatcgccgcc gccgtcgaga tgtccacgca
    51001 gcgcgcgtag tccgcgcgga cgccttcctt gccctccagc aggccgggga tggacttgaa
    51061 ctgccggcgg acctcttcga ccatggcgaa ggccgcctgg ccgaccgcgt tcatggcgaa
    51121 cgcggagaag aggaacggca tcatggcgcc gagcagcagg cccgccgtca cgttggggtc
    51181 catcacgtcg atcttcgtca ggccgacggt ctggccgaac gccgcgaaga gcgccagcgc
    51241 ggtcagcgcg gcggagccga tggcgaagcc cttgccgatg gccgcggtcg tgttgccgca
    51301 ggcgtcgagc ttgtcagtgc gctgccggac ttccttctcc aggtgcgcca tttcggccag
    51361 gccgccggcg ttgtcggcga tcgggccgta ggcgtccacg gcgagctgga tgcccgtggt
    51421 cgcgagcatg cccaggccgg cgatggcgat gccgtagagg ccggcggtct tgtaggccac
    51481 caggatcgcc gcgcccagga ggatggtcgg gaacgcggtg gacagcatgc cgttgccgag
    51541 gccggcgatg atgttcgtcg ccgcgccggt cgtggaggaa tccgcgatgc ggttggacgg
    51601 ccccttgccc tccgcggtgt agtactccga gaggaagccg atcgccacgc cggccgccag
    51661 gcccgcgatc gtggcccaga agatgccgat ggactggtag accttcggct ccatgcccgg
    51721 gatgatcgtc agcgagaact gcgccggggc cagcttcgcg atgacgaagt aggacgccac
    51781 ggcggtcgcc gcggcggcgg ccagcgagcc ggcgttcagg gccttctgcg ggttgccgcc
    51841 ctccttggtc ttcaccatga aggtcccggt gatggaaaag acgatgccga cgcccgcgat
    51901 gaccagcgga aggagaacga ggttcatggc cgagcccgcg atgccggcgg ggaccgtcgc
    51961 cacggccgcg aggaccatgg cgctgacgat ggcgccgacg taagactcga acaggtccgc
    52021 gcccatgccc gcgacgtcgc cgacgttgtc gccgacgtta tccgcgatga cggcggggtt
    52081 gcggggatcg tcctccggga tgcccgcctc gaccttgccg accaggtcgg cgccgacgtc
    52141 cgcggccttc gtgtagatgc cgccgccgac gcgggcgaac agggcgatcg agctggcgcc
    52201 catcgagaaa ccggtgatga tcggcagcac ggtgttggtc agggcggccg tctcggaacc
    52261 gaagaccttc aggtaggcca gcgtcagcgc ggacaggccg agcaggccca ggcccaccac
    52321 gcacatgccc atgaccgatc cgccggcgaa ggcgacggcg agcgcctgcg gcaggccctg
    52381 ccgcgccgcg ttggtcgtgc gcatgttggc ggccgtagcc acgcgcatgc cgaagaagcc
    52441 ggacagcacg gagcagaaag cgccgacgac gaacgaaagc gccaccagcc ccaggctctt
    52501 gttccccgtg accttgatgt tcaggaaggc cagcagggcg gccacgacga cgacgaagac
    52561 ggccaggacc ttgtactcgc gcttcaggaa ggccatggcg ccttcgcgga cggcggcccc
    52621 gatttcctgc atgcggggcg tccccggatc ctgcttgttg atccaggtcg ttctcacgaa
    52681 cgcgaatacc agcgccagga ccccagcgcc gagcgacatg tataacaggg tcatgtccat
    52741 gtgaatctcc ttattattct ggcaaaacag gctatattcc cggccggata cctgaccgga
    52801 cgggccgaca ctctaggggg ccggccccgc ggtgtcaatc ccgcaggcgg aggccgaaaa
    52861 aggttgcatc cgccgccgga acccgggaga agggaagggc gcggagcaaa cccatgctga
    52921 aactgaaact cgacaggccc ctggtcgtct tcgacatcga atccaccggg accaacaaga
    52981 aggcggaccg gctgatcgac ctggccgtgg tcaagttctg gcccgacggg cgcgccgaat
    53041 cgttcgcgtt ccgcgtgaac cccgagcggc ccatcccgcc ggagtccacg gagatccacg
    53101 gcatcacgga cgagatggtc aaggattgtc cgaccttcca gcagatcgcg ccccgcgtca
    53161 tggacgtgtt ccgtggctgc gacctggccg gctacaacat cctcgggttc gacatcccgc
    53221 tgctgaccga ggaattcacc cgcgccggcc tggtgttcga cacccaggcc tgccgcgtgc
    53281 tggacgccca gcggatcttc cacaagaagg tgccgcgcga cctgccggcc gcgctggcct
    53341 actactgcgg cgagatgcac ctcgacgcgc acgacgccat ggcggacgtc caggccaccg
    53401 tccgcgtgct ggagggccag ttcgagcgtt acgcggacct gccccgggac atggagcgcc
    53461 tggacgagtt ctgcaatccg cggcatcccg actgggtgga ccggtcgggc aagttcaagt
    53521 gggtgggccg cgaggcggtg atcaacttcg gcaagaagca ggggacgccg cttgccgaga
    53581 tcgcccggac ggatcccggt tttctcaagt ggatgctgaa aagcgacttc gcgccggacg
    53641 cgctggagat cgcgaagaac gcgctcgagg gcaagttgcc gcggccgccg ggcgggtgag
    53701 cttcaatcca cgcgttcgac cgacagctcc gggttgacca cttcccgcaa ctgcgtctcg
    53761 atgccctggt ggagcgtcat cagcgccccg ggacagcctc ggcaggcgcc gaggagcttc
    53821 aactggacca ccttgtcccg aagggccacg aattccacgt cgcctccgtg ggactggatg
    53881 taggggcgga tctgctcgat gtaggccttg acctgttctt ccatgggcaa cctttcgcgt
    53941 ttggcctgaa cattccccat cgcgagggga tatgcaagcc gggtttttcc acgccgattc
    54001 cacattctct tgcttttgct cgaaagagaa acagtccatg agcgcgagcc tggttgcggg
    54061 gacgacggga ccgtgggagt tccgggacga gcactaccgt tgccgcggcc gaaagcgcag
    54121 ggtcacgggc gtattcagcg gcggcacgag cttgtcgttc acgaccagga tctcgtcgtt
    54181 ggagccggcg ggatcgccga tgttcacgat ggcccaggga tcccagtagg tgaccacgag
    54241 gctctcctcg gccagggcct tgaattcgcc gtcctcgaac atcgagccgg tgaacagcca
    54301 ggggatctgc ttgagaggcc ggttcctctc cacgttccgg atgaaccgct cggccggcag
    54361 cgagcgccgc ttgcccttga gttcccagtc cacccagata tccagggccg gcccggcggc
    54421 cccgggttga ccgagtccct cggggggcgt gcccggcttg aggccgagaa ggaggagccc
    54481 ggtctggagg tcaagcgggt tgaggtcgag gacgaaaacg ctctcgtggg ttttcccgcc
    54541 cttgccgcac gccagcagtt cgatcgcgcc cgagacctgg ttgacccagc cggtggccag
    54601 taccgtgcgg gtggtcgggt ccacgcgcac ggagccgagg gtggcgatgc cgtccttcca
    54661 ggcaggggcc gttggggcgg gctccccggc ggcggccagc gtggccgcgg cgagcagcgg
    54721 caggatcgac agggggcggt tcattcgatg atggacatgg cctctgccgc gcgccggcat
    54781 ttctccatct gccgccggta ctcctcgatg ttgcgttcgg ttcccttctc ggcggcggcg
    54841 aggtccaggg cgatctgggc ggcgcgctgg gcccgagggt actggcccag gatgtagtag
    54901 gcctcggaga gggtgctcca gacgtggtag tccttcggcg ccatcagcag ggaatcctgc
    54961 gccagttcca gggcccgggc gccgttgcgg atgctgttgt cctcggccgt ggcgtagagc
    55021 catgccaggt tgttcttcag cgagtagtcg tcggggttgt ccacgatcag ggcttcgaag
    55081 atgtcccgac cttcctggta ctgcttgagc tggatcatga tgttgcccag gccgaaccgc
    55141 gcgcggtgat tcttggggtc gtacttcagc acgctgcgga actgcgccag cgcatcctgg
    55201 tagcgcccgc ggttcatgtt cccgtaggcc tggtccagcc gccggctgat ctcgcggagc
    55261 gtctcgcgcg tctgggcggg ggtctccgcc gcgggctcgc cgggcgccgc gggttcggcg
    55321 gccttcgccc agcttgcgaa cgcgaagatc atcaggtccg cgtccgccgg ccccgcgaag
    55381 aaacccgcca gcagccagag gcccacgatt cccttgaccg actgtcgcag cacatgcatc
    55441 aaggctctcc ccatgtcacg ctcaccaccg ggggctccgt tttgaccacc gcgagccccg
    55501 gaggcggatg gatgcggagc ggcaattcgt acgtgcccgc cttctccacg tccgagccgt
    55561 cggcatacac ccggacgtcg cgcgcgtcca gttttttcag gatctccgcc cgtccgcgca
    55621 cggtgagcgt cgcggacacg gggacgaccg acaattcctc gcgcgcgccc ggccggctca
    55681 gcacgcagac cgggacgtcc ttccacgtct ccgtggaggt ccgttcggcg aggctgacct
    55741 ccacgctgac ctgggcgggg acgggctcca cgacgacggt ttccgggggc gcctgcaccg
    55801 gcacgtgcgt cttgcggaac gacttggtgc ggccctccag gtcgagcgcg atcgtgttga
    55861 ccgactcgat gcgttggagc cggctgtgcg gcccggaaag cgtgacggcg gccggcgcgc
    55921 actcccagcg ctcgacttcg aactcctccg cgggctcgcc ctggaagtcc gccttgaccg
    55981 gcacagcctt ctgctcctgc cggtcgaggc tcaccgtgat ttcgtcgggc cggacctgga
    56041 ccggccggac ggtgttgggc gcgctgatat gccgggggag aatcttgaag cgggcgacca
    56101 ggttggtgcc cttgatggcc cgcgggttca ggtcgatctc gaccttgatc tggtcccggt
    56161 tcaggaagcg gatgtcctcc tccgagcccc ggaacacgat gtccacggcg gagacggact
    56221 ggtccagggc ggcccagccc tccggcaggc gcagggtcac gggcacttcc cggatgcggg
    56281 cctcgaagtt gatgacggac tggatgccgt accaggtcac ggtggcgagc accagggcgg
    56341 ccagcttgcc gccccaattc cgccggagca gttcggcgag cctggaggcc cagctaggca
    56401 ttcgggcggg cctcctcctg ccgggccgcg tcggtcttgg ccacgccttc gggcgtcagg
    56461 tccagctgct ccttgacccg gcccatccgg ctctgggggc gcggcgattt gagcagcacc
    56521 gccgagagca tccggcggag gcgctcctcg tccagcccgc ggctcaagcg ccccttgtag
    56581 gcgaccgaga tcgtgcccgt ctcctccgag atcaccacga ccagcgcgtc ggtctcctcg
    56641 gtgagcccga cggccgcgcg gtgccgcgtg ccgagggcct tgctgaactc ctcgcgggcg
    56701 gagagcggga acatgcagcc ggccgcgacc agccggttgc cgcggatcag caccccgccg
    56761 tcgtgcagcg gcgtgtgggg atagaagatg ctcgccagaa gctcgggcgt gaccacgctg
    56821 tcgatcttca cgcccgtctc gtgcacggcc cgcgtgccga tctcgcgctc cacggcgacc
    56881 agggcgccga tcttccgttc cgagagcatg agcgccgcct gcacgatatg ctccaccacg
    56941 gtgcgctccg ccgccgcgga cgcgaagacg tgcggcttgc cgagttccgc cagcgcgcgc
    57001 cggatctccg gctggaagat gatcaccagc gccacggcca ggtacagcga cgcgcgcgcc
    57061 acgagccagt tgaccgtgtc caggcgcagg accttggtca gcacgatcat cacccccagc
    57121 accacggcga gcccgaagag gatctgcacg ccgcgggtcc cctggaagaa catgatgacg
    57181 tagaagaacg cgaaggcgaa cagggcgatt tcgagcagcc cggtgaggcc cggccactgg
    57241 atctggtgga accaattcac gtcgcgcttt cctttctcaa catatccacg acgcgaacca
    57301 tatcgcaagt ctcctttacg tcgtgcgcgc ggatgacgtg cgccccgcgc gcgatcgccc
    57361 aggcggccgc gccgagcccg cccgctagcc gctcgcccgc ctcccgcccc gtgatccggc
    57421 ccaggaagct cttgcgggag agcccgacga ccaccggccg gcccagcccg gccagcgtat
    57481 ccaggtcatg cagcagcgcc acgttgtgct ccagcgtctt gccgaatccg atcccggggt
    57541 ccaccgccag cgtctccggg gccaagccct gcgccgcgag ggcggcgatc cggcccgcca
    57601 ggtagtcccg cacctcccgg accacgtcgg cgtaacgggg atccgcctgc atggtccggg
    57661 gggtcccctg catgtgcatc aggatcaccc ccgcgccgta ccgcgccgcc acggcgggca
    57721 tcgccggatc ggccgccagg gccgtgacat cgttgataac gtcggctccg gcctcgagtg
    57781 cgcgagccgc cacggccgcc ttcgccgtgt ccaccgaaat cgggatgtcg ctttcggcgc
    57841 ggatcgcctc gatcacgggc agcacccggc ggatttcctc gtcccccggg acgggcgccg
    57901 cgcccggccg cgtggattcc ccgccgacgt cgagcaggtc cgcccccccg gcgatcatcc
    57961 gccgggcctg ttccagggcg cgggcgggat ccaggaaccg gccgccgtcg gaaaaggaat
    58021 ccggggtgac gttaaggacg cccatgacga gcgtgcgccg gccaagttcc aggcggcggc
    58081 gccgcgccgt ccacaggagt gtcggatggt cgttcatgtc ccggtttccg ctatcgccgg
    58141 gccttccggc tcatcaggcg ggggggctcg gcggggaggc gcccggcgct gcgcgctcgg
    58201 cctcggacag gatccggccg tgctcgatga tctccgccac ctcgcgcccg tccagcgtct
    58261 cgcgctccag gagcagctgc gccacgcggt ccaggctttc tcggtgcccg cgcaggaggt
    58321 tcagcgcctt gtcgtgcgcc tcgcggacga tccggctgac ctcctcgtca atcttgcgcg
    58381 cggtctcctc gctgacctcg ttggtgcggg aaacctcgcg gccgaggaag agcagttcct
    58441 cgcgttcgcc gaagttcagc gggccgagcg cctcgctcat gccccactcg gtcaccatca
    58501 tgcgcgcgat atgcgtggcc tgcttgaggt cgttgtgcgc gcccgtcgtg atgtccccga
    58561 agacgagttc ctcggccgtg cgcccgccca tcagcccggt cagggtggcg aggagcttgg
    58621 agcggctctc cgtgtagcgg tccttctccg gcagctgcat cgtcgcgccg agcgcggcgc
    58681 cgcgcgggat gatggtgacc ttgtgcagcg gctcgcattc ctcgagcaac tggagcagga
    58741 cggcgtggcc cgcctcgtgg taggccgtca atttcttttc cttctcgtcg agcacgcggc
    58801 tgcgccgctc gcggccccag cggaccttgt cgcgggcctc ctccagctcg cgctcggtga
    58861 tggcgtccag gttctgccgg gcggccagca gcgccgcctc gttgatcagg ttggagaggt
    58921 cggcgccgga gaatccgggc gtgccgcggg cgatgcgccg caggtcggtg ccggccgcca
    58981 gcttcacgcg ccgcgagtgg atgcgcagga tcgcctcgcg cccctcgagg cccggcaggt
    59041 cgatgatgat ctgccggtcg aaccggcccg gccggagcag ggcggggtcg agcacgtcgg
    59101 gccggttggt cgcggcgatg atgatcacgc cctcctgcgt gtcgaagccg tccatctcca
    59161 ccagcagggc gttcagcgtc tgctcgcgct cgtcgtggcc gccgccgatg ccgctgaagc
    59221 ggctgcggcc gacggcgtcg atctcgtcca cgaagatgat gcacggcgcg ttcttcttgc
    59281 cctgctcgaa catgtcgcgc acgcggctgg cgccgacgcc gacgaacatc tcgacgaagt
    59341 ccgacccgct gatgctgaag aacggcacct gggcttcgcc ggcgatggcc ttggccagca
    59401 gcgtcttgcc cgtgccgggg gggccgacca ggatcacgcc cttggggatc cgtccgccga
    59461 gcttctggaa ccgcttcggg tccttcagga actcgatgat ctcctggacc tcctccttgg
    59521 cctcatcgat gcccgcgacg tcggcgaagg tcaccttgtt gcgctcgcgg ctcatcagcc
    59581 gggcccggct cttgccgaag ctcatcgccc cgcgcccggc catgcgcatc tgccgcgcga
    59641 agaagaagta gatgatgccg aagacgagaa ggaagggcgc cacgctggac acgagctgcc
    59701 agatgtaggg gttctgggtc ttgaactcga acggcacgcc gctctcgcgg aggaaccgta
    59761 tcaggtcgtc gccgaccagg atctccgtgc ggaaccgccg cggccgcttg gtccggttat
    59821 ccttgtccag cagttcgccc cgcacgtagt ggaggccgga gacctccacg atgacctcgg
    59881 ccttacggat gcgctgctgc ttgaccagct cgatgaagtc cgggctgtac gggatgcgct
    59941 cgtagcggtc ctgctgctgg gagaacatct ggtagaccgt caggaagagg gccaggagca
    60001 gcagccagag ggctacgccc cgcagcggga cattcgtctt gcccgggccg gtttccttcg
    60061 gactgtagat cggaggttcc atgtcgtgat ccggtggggg aacgtaccat ccccccgcgc
    60121 atcatgcaac gccgatgcga tgtggaggcg gcccgccccg gggccgcccc cttccgtcgc
    60181 ggggctacgg cgccccggct ccggttccct acggctgccg aaccctgacc cggtagaacc
    60241 gccggttgtc cggcggcggg ttgctcgacg tgttcgccgt ctggtcgtcg gtgaaggtga
    60301 acgtcgtggc caccgttccc gtctccagcc acgtgcccac cccgaggttc gtgttgccga
    60361 agcccgacca cgccgtcccg ttcgtcaggc tgttccggaa gtagatcgtg taatgccgct
    60421 gcggctgcgt cgggatccgc acctggtagt tggtcaggcc ggccggcacg atgacggact
    60481 ggacctcgaa gaccgacgac acgttcgtgg gctgcgtgcc cgcgacgtac tcgtcgccgt
    60541 tcggcgcgcc gtcgctgtcg cggtcgccgc cggaaccgtt cgtgagcgtg gtgaagtggt
    60601 cccactccca gccgtccggc agggtgtcgg agtccgaatc gtagggcacc acgatcacgg
    60661 tgctgttggt cacgatgctg ttcgtcgcgc cctgcccgtc gttggcgacg aagacgaggg
    60721 cgttggtcgt gttcgcgaac gccgtcggca acgcggtcca cgagaagttc gtcccgatgt
    60781 acgtcgcccc ggagggcttc gtcacgttcg tcacggtcac cgcgttcccg tcggaatccg
    60841 tcgcgctgac gacgaagctc gtggtcgagc cgaccggcag cgtgtgcggg cccggcaggt
    60901 tgatcgcggg cgcgtggttc gtcgcggtgt actcctcgat cttcaggtac tggacgatct
    60961 cgccgtcggc ggtgatcgga ttgaccgtgc cgccgccgag cgcgacgtag atgccgttgt
    61021 tgtagaggtc cgcgccggtc ttcggccagc cgctggtcag gtaggcggac gcgtcgctgg
    61081 aggccaggtt gcgcacgcgg taggtcttgc ccgtgtcgag gcccagcagg ctccagcagc
    61141 ccgtcaggtc gaaagaggcg gccgtggaga agtgcgcctc gccgtggcgg aagaagatgg
    61201 agaaggccag caccacgtcg ctggtctgcg gcgaggcgta cgcggtctgg tacttcgccg
    61261 cggccatgat cgtgccctcc tcgccgccgc cgaccttgtt gaggaaatag cggttgtggc
    61321 tgcgcagggc gggcgagttc agccgcgccc agttgacccg gccgtaccac tgggccagcc
    61381 cctcggagta gtccggcggc tgctgccaca cggtcagctt gttccactgc ttgaagtgcg
    61441 ggatgtactt gccgaagttc agctcgtgcc agttgaggaa cccgtcgttc ttgttgtccg
    61501 ggtccgaggc gttgaactgc tcgatgcccc actcctgccc gtagaagatc atcggcaggc
    61561 cgtccaccat cgagaccgag ccgtagcggc tggcgacgac ccacgggtcg ttgtccgcca
    61621 tgacctcgtc gtgcccggtc aggttgagca ggacctcgcc gctgctgtag gagttgcgcc
    61681 ggtcctcgag ggcctggcga agcgagctgg cgtagttgat gtgcgcctgg gtgaactgga
    61741 agaccaggtt ctcgttgagg atgtcgaagt ggcggttcga ccggtagccc gggctgccgc
    61801 cgtcgagcgt ttcggccatg aacacgaaat tccacttcat cttgcgcgtg cggttgatga
    61861 tgtactccca gagctggggc ggcaggccct gcccgaagtc gcagcgcagc gagtcgatgc
    61921 cgtagtcgtc gtaggcggcg ttgttgttgt tcgtcgggtg gccggtcttc ttcagccagt
    61981 actcggtgta gtagccgaag tagcgccaga gggccatggt gttggtgtcc atcccggcgt
    62041 agtcgtacgt gtcgccctcg ttgttgtagt cgccgttgtt gtccgggttg tgccggacca
    62101 gggcggagta cttgccgaag tacagctccg cgacgtcgtc ccacttgccg aagtcgccgc
    62161 ggtcgggcgc ggtggcgatg tcgttgtcgt actccgagtt gtagaacgtc gccggcgcgc
    62221 cgtagtcctg ccagtaggcg taccaggccg gccgggtccg gcccatggtc tgggtggccg
    62281 acgtggcgta gcccaggtcc acgccgccct ggcccatcac cgcgtcccac gccgtgtggt
    62341 tgaacacgcc gtcgagcatg acgttgatcg tcccgacgct ggacgcgttg gtgtcgcagc
    62401 ggtggacgaa gttggtgaac tccgcgaggg cgtccgcctc ggtgtccgcg tcgcccagga
    62461 ccgacgcgac ggagaagtag tcgcgcgtcg cgtacgggct cccggggtcg tacccgctcg
    62521 ggtcgccctt cgtggtcacg gcgctcgggt ggatcggctg gaaccacagg cagttgacct
    62581 gtatcttgtt caggtagccg aggttgaagt tcgtgaagct gtccaagttg ccgtcgagca
    62641 ggtcgtcgaa cgtgctgcgc ccggccttgt cgttgctcgt cgccttcacg gtcagcgggt
    62701 tgacctcgta gagcgtcatg cccagcgcct tcttcggcga gacgacgatc gcgtggtcgc
    62761 gccggcc
//
LOCUS       DAOOTK010000006        60601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_398_length_60601_cov_6.743980, whole genome shotgun sequence.
ACCESSION   DAOOTK010000006 DAOOTK010000000
VERSION     DAOOTK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 60601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60601
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_398_length_60601_cov_6.743980"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..421
                     /locus_tag="PK634_01610"
     CDS             71..421
                     /locus_tag="PK634_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOW96462.1"
                     /translation="MSATEQGADPYFGRMNDPTASARLRGPCGDEMEFYLVIAGGKIE
                     DVRYYTDGCAHTRLCGRAVAQRALGKTAHEALGINPREIIETAECLPDEGNHCAILAV
                     STLYRALAAYLLEP"
     gene            433..987
                     /locus_tag="PK634_01615"
     CDS             433..987
                     /locus_tag="PK634_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOW96463.1"
                     /translation="MLKALHLCMNHGRRVKRYRHVPFAPGLNLLIGPNGTGKSTILQA
                     VSECPHCRKIEEGPTEYVLFDSEQMNPRVSDRPARNATDMVLRARALFSSHGEILQAA
                     FSTLRLSPETCLLLDEPESGQDFEHVLALRAAMDRTVARGVQVICSTHQVLFWDRAHF
                     IELRRGYRDCVMGSIRRVHGREST"
     gene            984..1844
                     /locus_tag="PK634_01620"
     CDS             984..1844
                     /locus_tag="PK634_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOW96464.1"
                     /translation="MKTTLDCLPCFARQAAEVVTMSVDDPARREALLRRLLREIAGTD
                     WQGSPPEMAQRLHRIIRRETGIPDPYRRVKDEMNRAALDLLPRLRDHLARQPDPREAA
                     VRLAIGGNLLDAGAKTQIGAEDLPRHLETIWPRPLRGRVDDLFRAAEQARCILYLADN
                     AGEIVFDRVLIEVLPAERITVFVRGAPVINDATMDDAVTAGLPEVAPVLDNGSDAPGT
                     VLADCSPAFREWFDRADLVIAKGQGNYETLSDVPKRIFFLFTVKCPVVAAHIGEPVGS
                     LVVKERLAHE"
     gene            1837..2700
                     /locus_tag="PK634_01625"
     CDS             1837..2700
                     /locus_tag="PK634_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW96465.1"
                     /translation="MSSFTVAIASGKGGTGKTTVAVNLAALLAERGVAVQYLDGDVEE
                     PNGHLFLKPALETSEAVGLPVPVIDADKCTACGRCAEVCEFNALVVLKRAMVFPELCH
                     GCGGCVTACPAGAIREEPRAVGVVETGRAGAILFAQGRMNVGEAMAPPVIRALKRARA
                     DGALVLLDTPPGTACPVVAAARDADYVLLVTEPTPFGLHDLRLAVELMRRLERPFGVV
                     INRAGSGDDAVNRFCREAHIPVLAEIPDDRAAAEAYSRGELMNGLPGFQQALDRLSER
                     ILSEMSEKVRA"
     gene            2697..3578
                     /locus_tag="PK634_01630"
     CDS             2697..3578
                     /locus_tag="PK634_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW96466.1"
                     /translation="MKELVVISGKGGTGKTSVTASFAALAGRAVLADCDVDAADLHLL
                     LRPDIRRRESFHAGHAAVIRRASCTGCGACAAACRFDAIRAADGGTFRVDAAGCEGCG
                     VCVRVCPAQAVDFPERVAGEWYVSDTRHGPMVHARLEPGGENSGKLVARVREEARAVG
                     RAAGYDWLLVDGPPGIGCPVIASLTGASRVLVVTEPTLSGAHDLERVLGLARHFNVPA
                     LVCVNKWDINPAQTEAIERRVSALGARLAGRIRYDRAATQALLAGRSIVEGPPGEAAA
                     DLRALWNAVTNDVRKER"
     gene            3598..3936
                     /locus_tag="PK634_01635"
     CDS             3598..3936
                     /locus_tag="PK634_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOW96467.1"
                     /translation="MKIAIPVAGGKLCAHFGHCEQFALVEADEKTRKIAGTALRDPPP
                     HEPGVLPRWLHEQGANVILAGGMGVRAQQLFAQNGIRVIVGAPCAEPEALAAAFLAGT
                     LQSGDNLCDH"
     gene            3944..4966
                     /locus_tag="PK634_01640"
     CDS             3944..4966
                     /locus_tag="PK634_01640"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOW96468.1"
                     /translation="MNEAKQAATLLDREIVQVVGCTEPASCAYAFRAIARHWPGPLDL
                     KTLRARLWLSHDVHRNASTAVVPFLHRRGVRAAAAAGLATDAAGFNVFPCMNLAKART
                     LLRRRGWLKIIRRRQRGIWVRAELVAGGHRGKALITERHDRITRLVVDGREIRMKYPP
                     AVVPPGLDEIVRLARRRAPALEAVAEDFLLKQALGQPRLPVEKRVARLVRERMCGYRH
                     AIMTITGSGNHGIFLGLPLRELYRERGRATLPAIVLALLVQLRMSHERSRISDDCGLA
                     LKAAPALAAGLAYARGADAPALRRVIRAVTRDLRSLECRGARASCGGKAEKALRVVRK
                     HVEALA"
     gene            4963..5793
                     /locus_tag="PK634_01645"
     CDS             4963..5793
                     /locus_tag="PK634_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOW96469.1"
                     /translation="MNDAVRITVLVENGAGAPNLAAEHGLSLWIETPAGPVLFDTGQG
                     PALPSNAAALNIPVADARAVVLSHGHYDHTGGLAWFLEQNRSACVVLDPEAGKERYSL
                     RDGVRAIGMPAPARAALAAAGGRVTWSAQPVEVAPNVWTTGEIPRATDYEDVGGPFFL
                     DAGGKTPDRLPDDQALWTECARGLVVVLGCAHSGVVNTLEYIAKLTGTRQFHAVLGGM
                     HLAAASQERLAATAAALTRYDVAVLGPGHCTGDAAAAYLAEHTACRLWPCHAGKVLTL
                     "
     gene            5925..6989
                     /locus_tag="PK634_01650"
     CDS             5925..6989
                     /locus_tag="PK634_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96470.1"
                     /translation="MNRLTELCRLVALAAAISGLVSCGKKAEPPPPAQTEEAVAPTPE
                     VEDGPVRTAFRGETPLLDIAVVPPASTNEEPLTLVAAGESRTVLRSTDNGETWAPVLP
                     AEKDGPDFSAVLFPTTADGWLLFREQVLHSSDRGATWTAAAVPERGFYYFGSLSAVGS
                     QCYAIQPPTCGAKVFRTGDGGATWTALEGGLPRNDYGAVFFRDDRRGWVVGPYGRYAF
                     TEDGGATWKTKDFPSEIGWAELEMVTDDFGWMRADRGHAGRLWITRDGGSDWETVDLS
                     IQGYWNIVDVDFLDPDTGLALAQKGADGSLVLLSRDAGATWAVWMDVKPVLTAMAFRD
                     LDHGWFTSADGRIYKTVPAP"
     gene            complement(7014..10043)
                     /locus_tag="PK634_01655"
     CDS             complement(7014..10043)
                     /locus_tag="PK634_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HOW96471.1"
                     /translation="MKLGSRVLLALGPSLAVLFIVLYAVGTHGLRAGFADLEQRFALQ
                     NVQQVQWAVEAEGRHLDRSVADWAFWDDTYAFVEDRNEAFIESNLTLDTFQSLGIRLL
                     IIVDPSGAVVWGGIRDPESSAVRPLPDDAARWLAPGAPLARGEEGDWSLAGLMSLAGE
                     PLVVAARPILTSQREGPPRGTVVMGRALDAAWFGELAAILHLQVNVYPARALPSHVTE
                     SGAWPALLRGAPAAILPLDENTMRAYALLRDLEKQPAAVIEIRRPRMIYAVGLRIARF
                     GRLMLGLVALVFISAIVVLLRRLVSRPLANLRRDIDEVSASPGFTRRVPRRGPSELAG
                     VADHINRLLDLVAESHRFLDSIIENIPNMIFIKDARELRFVRFNRAGEDLLGTTREEL
                     IGRSDRDIFPKEQADFFIAKDREVLEGRKPLDIPEERLQTRRKGARILHTRKVPVLNE
                     RGEPEYMLGISEDITEWKRVDEQRRATEAEMQRLLEAARKSRKALLSVVEDQKRTQAS
                     LTALSFRQEALLSAIPDIVMEVDARQVYTWANPAGLAFFGADVVGRSASSYFAGEQST
                     EDLVKPVFEGSREVVYVESWQRRQDGQKRLLAWWCRGLKNDRGEVIGAISTARDITEQ
                     SVLEEQVRQIQKIEAIGRLAGGVSHDFNNMLGVIIGHAEMAMESIEPGQPLLENLQEI
                     LTAARRSADLTRQLLAFARKQTITPRVLDVNETVAGMLKMLRRLIGEDIELVWEPGPD
                     VWPVKMDPAQMDQVLANLSVNARDAMKGAGMLTIRTENVVVEESDVRHHPGSYPGEFV
                     MLTVRDTGTGMDKATMEHIFEPFFTTKEAGKGTGLGLSTVFGIVKQNRGWIEVASRPG
                     QGATFRISLPRERSAAEEEAVSAEGSAGGTETILLVEDELPILKLGEKILKKAGYTVL
                     AARAPGEAMALAAGHPGRIHLLITDVVMPTMNGKDLRDKLAETRPDMKALYISGYTAD
                     VIGQHGVLDEGIQFLQKPFTGRTLNAKVREVLDRKKA"
     gene            complement(10076..11077)
                     /locus_tag="PK634_01660"
     CDS             complement(10076..11077)
                     /locus_tag="PK634_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW96472.1"
                     /translation="MKILVVEDDENSRVLQHTILEGNGYEVVSARNGREALERLKEAI
                     PDLIVSDIMMPDMDGYALCRTLKSDPVLARIPLVIYSATFTTEEDQQLAREFGADLFL
                     VKPMDQDAFMEQIRRVLASGVRTAPVRRPSARDKDRLDAQYIATIARKLDKKVLELND
                     ARARLRSSEERSRLFLDAIADMAFIKDEAFRLWYVNDAYAAFHGRPVEAILGRTDYEL
                     SPREAADRWRASDRQVLETGRPATDHDERNGRMYETRKFPVPLEGGKTGIGGLVRDVT
                     DQKRMEAAVRDQLDELRRWHDGTMGREMRVIELKNEVNELLARLGEPPRYGAADGEE"
     gene            complement(11074..11445)
                     /locus_tag="PK634_01665"
     CDS             complement(11074..11445)
                     /locus_tag="PK634_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW96473.1"
                     /translation="MKRVLVIEDNENNMELITFILETHGCSVLKAVCGREGVEKALRE
                     RPDFIVLDIQLPDILGTEVLKRIRAEEAGRHIPIIAMTSYAMAGDEQRLLAAGCNGYI
                     EKPIDPERVIGQITRIVGELS"
     gene            complement(11442..14372)
                     /locus_tag="PK634_01670"
     CDS             complement(11442..14372)
                     /locus_tag="PK634_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOW96474.1"
                     /translation="MKRNGNTVARGVVAAGLLLAAAGPGRAAEAVRVGAYENLPKIYS
                     LPDGRVSGFFPAILEHIAGQEGWTIEYRKGTWKECLDRLERGEIDVMPDVALTPERQQ
                     KFEFTEETALISWAVIYTRPDVVVQSFLDLADRRIALMRGSVYTSGTGSLPELLAKFD
                     IRAEYVEFESYRDVFQAVSRREADAGVVNNIFGSYFEKEYNVARSPVLFSPSQLRFAF
                     PKGSSQGARLKVALDRRLRELKVDPQSFYYRAMDTHLYGAPHGETPAAAADWTAVLTD
                     GEKNWLRRHPVIRVGIDPEFYPFEFRGPRGEYQGIASDYVRLFNERLGLNLRLVEGLA
                     WTAAVAGVRSGTIDVLPCVGRTLEREQWLRFSQPYIRFQRVIMTRLDMPFLAGLKDLE
                     SLRVGVQSGTSHEGFLRENSPAEPVLFGSLEESLLALSGGKVDAVVANLASAAYWIRK
                     LNLINLKVAAPASPEQYTLHFAVRKDWPELLGILNKALDLVTADQQREIEQRWVAVEY
                     KPGIERRVVWRIGLRIAVGVAVVLLVILFWNLRLKKEIGRRREIERRLNYRERFERLV
                     AETSSRLVAVKPAEMDRHIQVALAEIACFTGTAAGYLYAFGPQERAERTHAAGDLEAL
                     RAAEPGDRDEEWLARLRAGRPVTVSARADGEEHLVRPIRGRVVEVPCATEEQVTGFLG
                     LLDTRPEAPPWSDEDISLLRLTGEMLSSALRRKQIEENLERYAVELERANRQLQDLDQ
                     LKSLFIASVSHELRTPLNSIIGFTGVILKGMAGELNDKQRDQLQRVYHSAQHLLALIT
                     DIIDISKIEAGRVDVYPQVFRLDEVIREAVESIRPQAEVKSLRVECGPLPELEVFTDR
                     KRLRQCLLNYLSNAVKYTEAGSVRVEARDLSERFEVAVSDTGIGISPEEQAKLFEPFV
                     RLDSHLRIKAGGTGLGLYLTRKIAREILLGEIEVRSEKNRGSTFILRAPRRRPAQPGE
                     EKPS"
     gene            complement(14478..15569)
                     /locus_tag="PK634_01675"
     CDS             complement(14478..15569)
                     /locus_tag="PK634_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96475.1"
                     /translation="MKGRGLLVVLLAALLGGPEARAQTNEPAAGRALVEATRKAKVRL
                     WGQVNRALLYAENGSFSEWFHVDNDSSPSRLGAAGEYTPDGWPDWTFGGQVELGFKSD
                     NSAAVTFGGEDPEFEVDGRKIEAYARSPSAGSLALGQGDMASNGSAEEDLSGTYVISY
                     SQVRFAAASLVFGTNGPAIKEVFNNFDGLHRDDRLRYDTPAWHGWSAAASAAGQDFYD
                     AALRFARDLGPCQGAAALAVAHKGADVDQFSGSCSVWLDGGLNVTAAAGGQEVEGDYS
                     PFSWYGKIGWRLDWLSCGETCLAVDYSRNEEISQAGDRAESRAVVLNQRLEPIASDLY
                     ASVRTFTLDRPGEEYEDVLTVMAGVRLCF"
     gene            complement(15735..17360)
                     /locus_tag="PK634_01680"
     CDS             complement(15735..17360)
                     /locus_tag="PK634_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96476.1"
                     /translation="MRRGRIIAGSGLAWLALAWAACASTNIAYIGGDPDLWNLAEIQT
                     ALADLAPSPIPDLNAFIAALHDYADSDRFAYGGTDAERLDRISAEPVPMINEVIVSNT
                     FRLVQAGTNTLLEHFLHLTVETWFPFPADPANPEFQVAYSGALVCQVMPIAITNPLAL
                     QSSPPAIQHEGHDYKLSTFTFSHATPVNGPGQPVYPPPVIGVRVILEDSIQAQYLGTA
                     VDRVWRGWPATCFQVIGPAPLLVVGGPSMPFGPPAGCSANDPRINWNPALSVHWSSAV
                     ATPGQPNVTLSGPSNSVGFMFAATNGIISVAELTNVLYDAAKPWTTVTLVDPDPDETW
                     RIRNQLTVNERFPLVADAGPHGQVSPLQTNVLSGEDAAFLITPDLYYTLDSLWTNGGA
                     AGADLGTGVHEFVWSSVLFTGAFHAVFAEVRAANFGTPYPWLAEHGFTTDMEAAELLT
                     GSNGMPVWQSYVAGLDPHDPDAQFEIRSLAGEGLLFDTATGRLYAVDGESNLAEQAWA
                     ASLTNDMPGTGGPVQVPVEPSAGGVYRLRVREP"
     gene            complement(17376..17561)
                     /locus_tag="PK634_01685"
     CDS             complement(17376..17561)
                     /locus_tag="PK634_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOW96477.1"
                     /translation="MKFKVDHDLCIGCGACERTCPAVFALVDDKSNVQLDPVPPEHEE
                     KALRAEDGCPVGAISHE"
     gene            complement(17618..18001)
                     /locus_tag="PK634_01690"
     CDS             complement(17618..18001)
                     /locus_tag="PK634_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW96478.1"
                     /translation="MNTDGRSETAATILVIDDNSCIRDLLARLLRRYGRVECAATVDE
                     GLQLFRRLGPGLVITDYEMPGRNGIDGIRAMKALAPGVHIVMLSGSATRELTDEAKRE
                     GAFECLGKPFELNVLRELTARCLAV"
     gene            18215..19792
                     /locus_tag="PK634_01695"
     CDS             18215..19792
                     /locus_tag="PK634_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96479.1"
                     /translation="MKVLTGVGLACMTATGALGASPPFEEIWEAAGSISPDYNKNTHL
                     FGHAVAMSGPRMAVGRPGVQLGGAVNCGKVHIYTLDSNGREVANTLRVLEPSSAANDV
                     LSGAFFGAAVDLKGTQCIVGAPGNVRMTTAAQDNRNVYAGAVYIYEGNNWQRTARLRP
                     AAGAPRTSKFGASVGIDGEFAVVGSPGESSVYFYRKVNGAWNQVAKVTKAGFRFGTSV
                     AISGTRILAGAPEEEAGRGCVYAYQWSNNQWQQAQRIRGVIAGAQFGHSVAMDGLRAV
                     VGSIAENTYRGAARVYEFSQNQWQWKARLASETDVDYQAFGDSVDVSGSKIVVGSLAD
                     PWDDPTTGETYVYGLAYNAWNVLYYKHLPWQNGDYASYIHDVAIDGNLAAGSEAIDAA
                     YGDNDRNLGRVDVVMFDHHEVFFENVREATYSLYTPGSVRQGTAVLFNIEITWDARVP
                     AADRVLWVQLWDVRGMTSIYQHSFSAGPSLVANGAGTSSALAGNMPVRFRVWPARALP
                     AGVPGPTVILHAQYTLQ"
     gene            19975..20598
                     /locus_tag="PK634_01700"
     CDS             19975..20598
                     /locus_tag="PK634_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW96480.1"
                     /translation="MSEISPEQLGRLQSGDRRAWDDFFAQHDELIRSVVSWSKWRFSP
                     PTREDMAQGIRLALFKAIESFNEESSVEYYVKRICINRCIDEVRRQIRARQILVPLAT
                     GDGQRPEEETARMADRSQDPLRDVINAEVAGQVKRVLEQVDELCRVAIQRFYFAQQSY
                     KQMADELGISINTVGSRLAKCLEKMRKMFSADLFFGEDFRVSNDSGQ"
     gene            20613..21395
                     /locus_tag="PK634_01705"
     CDS             20613..21395
                     /locus_tag="PK634_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96481.1"
                     /translation="MSETDKLWALVHGQLNDPQAAELREALKADPALQRHYRQIQDMD
                     RLLRETGSQAEVPEELLVKKVLHAWETSPEAGRWGAAAPDASAPTRVLRFPGEALLWL
                     RQPWRALQTAATLAACALVLLGARNYFSPSLAWQPDRLEAAQYRGDKPADTGPVVEAL
                     RAMAADLRAEIASHYERQAGRTASRSVLRKRTEWRLATRIQELPESMLSVQVDAFRPG
                     DPAPEIRWTRQFENAEAFSRDLPEFGGQVASDLLRAARPAQP"
     gene            21405..23429
                     /locus_tag="PK634_01710"
     CDS             21405..23429
                     /locus_tag="PK634_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96482.1"
                     /translation="MDARRFFRSLAAAGLAGLAAGCAPTPPAGNSAPPPHKTWWNFYA
                     RGAALSYAGDYAAAAQQFRICLGLERGAVYGYPRDTWRARTYGFHFIDDYFPHRELGI
                     CYFQLGQASNAVRFLERSLQQQPSGRARHFLNRARAELVRGRVSAPPRIEADSPPPPW
                     SRDRTLLVSARVSGKGYVQDVALNGKPLFNELAVPVLPVEQAVDLAEGANVIRLSARD
                     LAGREARAEWTVNADWQPPALAVLGAERRADGWAVRARGSDRFGVARITVDGKPVTTD
                     GGTQQDFEFVAPLSSPLPVQIEDFAGNSLIRLFSAETLSPLAQARAPRYAARQPEGRP
                     DTPAAAAPDEGDRLPPGLRLHTPLRTRVFNSEFLIEVTTTDRGGLKSVTINGEDQLPD
                     ASRGCEQFHFARRCSLAPGTNTYDVVVTDRAGRTTREECVVVSQSPEYLDEEYRLRLG
                     VSPVSTALGAEPVVDDRAERVGRMMEHELLLYPPRFRVLERAEGWDALIEEQRLSASD
                     LADPRAALRVGKLMPAELLAITLLIPQGGSLTLFSRVVDTASGEILCAEDVYVPDPDR
                     DLADRVEGLVMKIEQQFPLLDGKITEAAGDIATVGIGALHGLKPGMRFLVLDWPGRER
                     GPLEESAVRRYSEQAVELRISRVYAKKAQARVSPPEAAAGVAEGDVIYAR"
     gene            23452..25266
                     /locus_tag="PK634_01715"
     CDS             23452..25266
                     /locus_tag="PK634_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOW96483.1"
                     /translation="MKRTAALLLALGLCFGLVFYLGHSASRYRGAAPVEPGPAARPGP
                     LAFHAVVIGASDYSAAGGRGWEDLQTARADAEAIADVLQRRYGFTVEVLTGQRASKAG
                     ILTALDRLADLTDRDAALIYYAGHGHYDATLDEGFWIPADARRTVDGRDAREDWIWNS
                     MITKMVNASRARHILVVADSCYAGSLFRGDGMPAEREWTWYARAFHRPSRYLVASGDL
                     EPVLDSGIEHSMFAGAVLEFLENPVSPVFSASDMALAVRQKVGELTGQMVRMGPLAVS
                     SHAGGEFVFTSDPSRLELADVAADPSAQQRSPETAEPPGDLETARDALWMQQAGLAQS
                     ARRLTAEASPEAKPVTAAVAAYLDPERRRGRQDALRELIEQVREQPPRADAADRVRPR
                     VLACLGPDPTPAGGEEQVMARLIRICLTAALQGQTRIVAVERENLEAALQELQLGVSS
                     LSDAQAQLQVGRFLPASILLLGDMARQDGRWVAWLRLVDTETSRILKSWNTDLDPANL
                     QENCREIAADIARAVAEMRPLEAAVTRAAQGGYRVAIGSFHGLDPQAALDVLADGKPA
                     GSAAVTRLGIMESDIAIEFAGGGKPGQNTALRVRERQP"
     gene            25320..27074
                     /locus_tag="PK634_01720"
     CDS             25320..27074
                     /locus_tag="PK634_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96484.1"
                     /translation="MPRSRIGICMLALGSLLGTQAARAEDFVIETPAGPVTISLDHAG
                     PQGSLVQGGAPSSPAFRPPSIFSAPLPSGSGARALGLAGAFTAVADDATAASWNPAGL
                     IQLERPEASVMLRASRERNAYNSDSDAFRVGVNSYDDENLNYLSCAYPLTLLNRNVVL
                     SINYQEAYDFNQRFSAAASEGRPPSSSASTETRVDTSTQVDHFENELDEGVTASITFV
                     THLTTRHTSALRELLQADLVNDLEFEQEGIIDAVTPAMAVEILPQLSLGLAVNMYQDS
                     ALHGIRSKTTASYTGSSTLDSAAQETRVTSSTYEYEGLIRYPPPFPEIPFSSSGEFPD
                     FTDSSTSGDTSGVVVDGVYEEENVLDDLRGFNTTLGLLWTVSRFLSLGGSVDLPWVAD
                     AEQTKTIRDTATTYNAARTRVLDRSSTESLVSKDVEITFPAYFALGAVWRWTDRLYTS
                     LDVSQTLWSDFAFQADGEEKINPLDGSPRGENPLDDTWAVRSGIEYLLVFPRVEIPLR
                     AGVAWEERPAIGEPDEYWSASVGTGIALGREPARWILDAAYVFTQGDDVLGSLVTDQD
                     LRTDVTEHQFFVSCIKHF"
     gene            complement(27416..34153)
                     /locus_tag="PK634_01725"
     CDS             complement(27416..34153)
                     /locus_tag="PK634_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOW96485.1"
                     /translation="MNATVRWRKGRVCRPASGSALLLGIALALFHFPPTSPAEILFQD
                     DFESGALAPCWATNSAGQGRIRVTGEWSPAGGAWHAVMDDVQSDLAPSLNELVLTTDL
                     AGRSNVVLSFRHKSLNDEDHEMPDSFSGSCNADGVAVSDDGGATWHKVQGLTSSNFVS
                     SSYKPFEVPLVPDGSWPLCDGFKIKFQQYDDSPAEEDGFAFDEIAIETMAAAPPADLS
                     VSLEPVFWGPGRAVSNHYPLLVTVGNAGPREAVEVSITNRLPPGVGVVTASLGYEFRP
                     ADPAVVFRLTEMAAGQTALYWMVCSPAVTGYCAWTSSVTSAGGDPDAEDNAAAFATIF
                     LPPSGPAVGIGVEVTLSEASCLSRPFTNKVHLENLSAEYVSNVLIVSEFSAPVEILDG
                     YFRANGNQLCSSCTLTPSWGASFSYAIRATRPGLLTNLVRISADRPLNSTNGLVITNV
                     IHILGDSTWEPVLDGYVDRRYLAGAYSGGGRSTKPAFGDMDADGDADAFVGSRDKLMY
                     YKNIGTPRSPAWAPPSLVRRLAWNTDYAPALADMDADGDLDLISGELFGMLYYFQNQG
                     TPTTAVWAAPVFAYGGITAGSFSAPALADVDGDGDQDLFIGNSDGDILVYTNIGTAAS
                     AAWGQPRTNFPCPIANYRYASPRPADVDGDGDLDLVAGYEPYPPGSTNAFWLLWANTG
                     TAAQARWAPAVTQQLGIAATDFFASFAPELADLDEDGDLDAVVGQAGGRMDHLVNIGQ
                     ATQAAWQVRGERIFLMDLPGDSMAGCDLDGDGDGDFVVLKGVERLLWLYENEGTPQTP
                     MWRLADTNYLSWTGGVLQAVSFADLDADSDPDLYIKMSGDPYGTNWFIARVLNTGTAS
                     RAAWAAPEYGYLVLHAETNSYYNAGQMAFGDMDGDGDLDMYLKSGSTNGPFFYVANTG
                     TASVAAWAPPVAAGAGFFGDSIEAPLVMDMDEDGDNDLIGMTGRDWVYYPNIGDAQQP
                     RWTHAMRLYEPPDTYGLNDFVESGAAMDLDADGDLDLLLATSEGGLLFYRNQADKLQV
                     IPAQRTMEPGSTCDFNVTNDPPVAWQFVANRSGGSLNAGTGVYQAGPTQGLDIIEARL
                     VDGRYGRAVAQVVSNSLSAGQARAIVMAGSKTAALTDDPAWEATDYLADNAFNTLRAR
                     GFSKNSIQYLSPLAGKDVDGDGYADVSRASTWANAEWAFRTFASGASNLFVYLVDHGA
                     TNEHGGYFRLNPGETLSATGLAAWLDELQNAAPMDVTVVLDFCQAGSFIGPLAYTGAA
                     SRVVIAGAGPVQAVFFVAGGLVSFSEAFFNGLRLGLGLGEAFQLARGAMSDYQDAWLD
                     ADKDGLFVAGVDDAGAGLDEPVGARIGGEQDVPQIGSIAGNQYLDGDSAATLWAANVT
                     AAQPIDRVWCVIVPPGYNPDPDVPIASLPEIDLVFTGERYEAVYQGFGQADTYKIIYY
                     AHAQGGGVSLPKYGYVTQAGADERAILAAGGTTNLAVWTNINGMANNAWETLQTRQFT
                     TDSVYYLSMETGQPGVDEAVSLTALGQAITNWAGASRKLAIYLAGEATGGQLHLAEGE
                     DLSAAQLDAWLDAYQQDSNRLVIAVMDFAGAGGFITNMTIPPDRDRILIAGSGPDEPA
                     LCEQQGWLSFSRIFLSHIFSGADVLEAFQAARDTTFNLARGAQDPQLDDDGDQEPNEK
                     AEGALAGVTYIGSAFVTGAEAPVIGAVTLDTLLEGTNALTLWASEIVAAAGVERAWCV
                     VTPPSDAEDPASVSVDLSWSNDTARYETSYTNFTEPGVYICTFFAMDHDGVIGSPRQA
                     QVLSADRYEPDDSFDEVQPFEVAGTQVHNLHVAGDEDWVEFFVITDFVYEVRTRQLGT
                     NVDTMLDLYRVEDGVLTNVVSRDSQGAGAGAGEYILLDHEPAGRYVARVRAYGAGQAG
                     PGTEYELRVYVPDAPAQAFLCILGADLYNSSRSPAGAVAYLNGTPYAFGGRNMIQFTP
                     IAAGTYTIQVTAPGCLPAQKATQAGQVENINNQAYGNPRRVVVQAGGFVSAPFLLEPL
                     ARAQGVARDEWTGRRLRNARIRFVPGSASGLPCSQYDGYPAYADYRQYWATSLEGAFP
                     SNVYLPTRAGVLTVFADGYSNLAFSLPTLPAGGVTNLGDLCCAPIPTNGNSLPARWIN
                     LYFGGPTNIAAGEDTDGDGANNWTELLTDTDPNDRASVFAIPEDGAVWGPGGWSFRWL
                     GAVAHAYQVASCSNLLDESWARIAGPWTNWDGSEMEWTAPDVEGRRLYRIEEFRP"
     gene            34443..37349
                     /locus_tag="PK634_01730"
     CDS             34443..37349
                     /locus_tag="PK634_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96486.1"
                     /translation="MPSTPRNKLSAWAAALCAALAVLPSIAIADDLVVLYPRIAHEVQ
                     APGVPIVDDGANRFEIYNDGLGFMSGGSLDAALGQVLTWRNFQAPDVVFDPIQARALL
                     LGGSRFGRASLYGVHFPDAAVNYATPIPPPFDGVDYTNSSRFSSLLVPARESCDAVTE
                     GFLWTCSSENQLTLVRYGWDGRAPAGRGTVVDLGARGYRYSWDRLSLPLDYSQDTRVL
                     DYDPAPGELWVLALTFHEEPGTMGAFSNSLLCLDGATGEIRAEVAGFRHVDTWEIDPR
                     NRTLWVADRSSIMGQDFIYRIDLAGLKAMRMNGLTRFAPDNPASTRLSSSAFGLEYIL
                     DMSVSPLDGAVWMLSRRAGTGYRLAKLDAAGTLACSRNFSDSGRAPLHDPDYLLADDA
                     GGCYVALGYAGNGSVLPSGERDYVRTSLARFDRNAALRGAGWQTRTVSNRRYRADRRL
                     ILLENAPCSEQQICLSAAPYCPRQRQTVSSRVLPSDCQGVSKIAWEVEKLDIGLGTVL
                     GLDDGFDTVWSDETAFSPCRASAHGRSEDLGPLGSGTYRFTVRVTGCNGTTGKNELWF
                     QVPAECPPTTPPGSDGVAVEPGDPGDPNRATLWIALDPVPAAVASSGGRPAYIFDQYL
                     RYRIQAAAPGDSIERLAWRWIHPADARRGAAQEVWDARTKDGWGDLASFRLTDCIGTD
                     RWGLPLAHDFGEYQLEVTLRLASGVDLTARSSKFYILGHKDAKVGIAPAPLYCADSSY
                     RVPLYAWSVPEYGFDTVDEFAVTCHHGLGQQDLRSIPYAPAMDWWPEEPGQAEGWWRI
                     PQFLVESDQPSITLKATATTLRSDGTPVPLSAETIVLLPCPINGLLGSTHAATAKCRK
                     FKAKMLMGSAEGRKIISAFYRLAPEMSALLAKDRVLSSQCAAFLKAVQPDLAALMQKG
                     NPKLSPLAPENLKLVTPKNIQAAKDIMTRVKPAASADLRSVIDELFRLLAAAGK"
     gene            37360..37524
                     /locus_tag="PK634_01735"
     CDS             37360..37524
                     /locus_tag="PK634_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96487.1"
                     /translation="MHSMTCPRGTLALLLALFRFAPAARDLPVKTRPYASSTLRTAPN
                     DLINKLDAAK"
     gene            37542..40259
                     /locus_tag="PK634_01740"
     CDS             37542..40259
                     /locus_tag="PK634_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96488.1"
                     /translation="MQRTHSTPTAWASRFLVLVWLLAGGAAAECGFPSDTAGWLVFPR
                     HRDAASPGGLWGANQLYTLDHSLAIRSTRTWSNASGQTPFWDSYWAGGPSFDPEGCRV
                     WMADGDQRSFYTVDIVTGSRHYYTVEAAADRDILFDKDCLWLAYDWIRERLWVSHNPW
                     IISEENHRIMKFRIEGTTLVKESDLDLQALGYELPAGPDENGIVRDSRSIRLDLVTGD
                     LWVLADTSEDGYSTPNILLSMDGQDGRVKCVLTGFDPGIDLWDLNEDEGSLWASVGNF
                     YGAGDGAGTLLKFDLRALPSGVYDGFSAAVLAAPLSSLYLAYPVDMAVSRVDGALWIW
                     DARDGLETGSNAQQHWRLARLSATGALTGSSDLTLDVEADTGITCSDFIETTANGGCI
                     AALAYTLPGGNESKVRTALYDAGLNRTAGWREVPIGTNWGFYGRGAALPRILTPPCLP
                     PLAHASTDRPRYRLGETVRLTCGALLRCEGVRQLAWSVRSSAGTEVGAGAYPAPGGCS
                     YADATQTVPLAGLPLGVYTATVVCTSCGSLPTTANCVFEILAAAPPRPTVSILREPYI
                     TAYGNRQDVRFRLRADAQGSLISELRWWAEDTNGVRAGAEQNAPAWGRSSYNERHILS
                     PLPPGDYYLKARLASDWGDVVEAASGRFRVWPFSKEARAGFDPDRYVILRYSDTCVPV
                     AGWAAADNGLSGYGVRWEGYGLAGDALPWRSFSAGWEPTADYWEPGCWPIPDAIWPNA
                     QTQDVAVFYLVGQAGAAGSPGIVSNRLAVILMCPLEEILGLESAATKSAREFRDLFLK
                     KSKDGKALADLYYKHARELSGLVAKQPALAKQAGALVTAALPDIAALLKSGKPTRGPW
                     AKENVKLVNAKTIAAARNLLAKTKPCASAGLRAALNDLINKLDAAVALR"
     gene            complement(40290..42539)
                     /locus_tag="PK634_01745"
     CDS             complement(40290..42539)
                     /locus_tag="PK634_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96489.1"
                     /translation="MKRRSVLRGVLVFLAVIGLQAGAQVSSQIHYQGRLISGTNLYNG
                     PATLLFAFYPGEVGGTALYTEQDVDVPVVDGLYATFIGDNPLSGSLPLALTRDEVWLA
                     LTVNGTPMMPRERVGAVAYALIAAAVTNQAISSAMLGPAVVTQAKIATNSIVNVHLTD
                     GVISNRNLAFGAVTSDKIGNGEVVPPDLDVGSFSNTFWTVGGNAGTRPPPLGADFAGT
                     LDDRPFELRVHDARVARFFPDASSPLIILGHGSNSVTGTGGAIAGGGSDSLPQFLGDA
                     YGFIGGGLGNTVSNGTAGVVAGGLRNHADAHFAAIGGGQQNLAQGPNSVTGGGSNNTA
                     RSSGSVVAGGGSNLALGAYGAVGGGQGNLSGAFAVVPGGRDNQASGDYSFAGGGGARA
                     SHPGAFVWADQQDGVFGSAASNQFLVRAGRGFGLNTTNLKGDIHFASGRTGQIATLVV
                     SPAATNANEDGAQILLAEDRGAIYGMYLQYEVDSEQLQILEKDSVLTNGPRLAVSRRN
                     NRVGVGTTNLMSALTVNGHILPDGSNLFALGSASYPWQKLYLASQIHYASPLGFVNGT
                     RTGLVVDAAGSLAVQGSVTITGEYRYAYTRTNYLWIPPQEFVPQHTDHNYRYSSGYLY
                     LTGTPKSASFAAGVHLPQDAEIAAVSSYCRNLGSYAMTCEVALARHEYEVSGIQYIAW
                     LQQVAASSPGSDAEIETDTAMMSPYDIVDNENTRYWVEVELSVGTFDGVNQQFHGVRV
                     EYRTDALRY"
     gene            42866..44038
                     /locus_tag="PK634_01750"
     CDS             42866..44038
                     /locus_tag="PK634_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOW96490.1"
                     /translation="METCAGITIREIKPGKWQTDLFRDGKRDRRVHENLEMAKRYAQL
                     LATKIRNEGSSALDLTQEQRRDALKALAILQDKTTLASAAQFWSLHNHNEDGVTIEQL
                     GERWLADLRRRGCRETTLRERKHKITRLTADFGNRPAASLTRQDLLDWLDRQGLTGVT
                     ADGYRRAYKTMFNFAMDEKIVEMNPVAGIKAFKTDERLPVPFTVASVQTIMDAAAKHA
                     PIMVPTLAVQFFAGLRPGEAKGLKWEDVNWTERTIRVLPETSKMRRSRLVEMNDTLRA
                     WLQPYRKKDGPIGITSQNQFDFYMSRKYCGEKKMGLLKAAGVEWIQDGPRKTFASMHY
                     ATHQDAAKLAAILGHTGDAGVLFRHYRGLVKPSEAANYWRLRPRTKTTIITLEATA"
     gene            44035..44652
                     /locus_tag="PK634_01755"
     CDS             44035..44652
                     /locus_tag="PK634_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96491.1"
                     /translation="MNKARSAHTRRFHDRAVELIAYYEDSAGDWSEIQQLFFALETSF
                     DPAFYITQRRPGSSPARIQLQRDKAIAAKQAKLAQALWAFDLGVAKQYADILNQCEKP
                     PGISGVEARSPFLHEIGKLKSPSLARQETERRKRSRDAKKRGKLGGRPLKDGNEEKLS
                     KALREARRMKRSGLSLRRACELAAKESGLTVGWEALKKHFYRGNN"
     gene            45119..46075
                     /locus_tag="PK634_01760"
     CDS             45119..46075
                     /locus_tag="PK634_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96492.1"
                     /translation="MNDVLISTAEASSRRLEDLREQLGMDSSPEDRIVVLGHLLTWYA
                     KWAGDPETDSGLFWNVTEKRLGVWAQWTRSNPLMFGRALVKAGYVQPVNDVFPEQLLR
                     KRDGYVVPGALDRSWSIHRLRANKLDLVLFLLDPVRRARQAAFLGLKPTDAPTGWLDF
                     SRLSGDVPGPGEGAYPSSGGPPEDLQSNSVGASESPPRGSPHVDVNVEKKKRMFPRKY
                     VGNKTTTGAPKTRDMLEYIQENKYENPLECLRTIDGTDGAVPIWIKGVEHCLSDVQTM
                     LANMTETDEAWAKIGNPAAVAMSRIKAVLRARGIRTGNGQQA"
     gene            46624..48117
                     /locus_tag="PK634_01765"
     CDS             46624..48117
                     /locus_tag="PK634_01765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOW96493.1"
                     /translation="MTLQELKSHLWNCAEILRGSAVDRTDWKAYILPLLFFKRICDVW
                     DEETSAAAKVYGDVDPVDFPEVHRFSVPAGCHWSDVRETPANIGAAFSHAMREIERAN
                     PDTLYRVFGAADWGNREMLTDGILKDLIEGLSEVSLGNAAVSTDILGDAYEYLIGKFA
                     DVTKRKKAGEFYTPRSVVRMMVKILDPKPGDAIYDPACGTGGMLLGAIEHVKQNGGDP
                     RTFFGKIHGQEKNLTTAAIARMNLVLHGIEDFKIVREDTLRSPAFTDSSTGGLSTFDC
                     VIANPPFSLKEWGREIWEADPWGRAAFGLPPDSYGDYAWVQHMVASMATRTGRMAVVL
                     PQGALFRKGAEGEIRRQLLERDLIEAVIGLAPNIFYGTGLAPAVVILRRTKPVAHKGK
                     VLIVDASSLLREGRAQNYLDPEHAEQIVAWVRAFKSVEDRAKVVILDEIKAEDWTLNI
                     SRYVLPPVAEDIPPLPEAVAALKAALTEARAAEDRLREVLTTGGWLK"
     gene            48212..49261
                     /locus_tag="PK634_01770"
     CDS             48212..49261
                     /locus_tag="PK634_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96494.1"
                     /translation="MNDKSKNNRAADLVSDSAIVEVKASLTAARGLRDALTSLAITLA
                     EQQQKRGYLLLLDPGLSKTFLETELQRLKVALRPGIAERLHLVAAKGGKLTEGTHAIP
                     QDDYMLLRRALNRQEDQRTGLPAPNKQNEVFLLMIHQWVTGKGPMTSKWLEDTVGCHY
                     RTVANAIDQLGHAVQRHSDRSVSFKYFPEQDWGRLLAVAQKTRATMLYADASDQPRSP
                     ESLLRRMKSLNRKDIAVGGVLGTKRYYNELDIVGTPRLDLCIHCPDNRVDLGFVHMLD
                     PALERTRDTHRPARLAIHFVRRQDALFDRDQNGAQWADPVECLLELYHARLDQQARSF
                     QEFLSARGRELSGKS"
     gene            49248..50192
                     /locus_tag="PK634_01775"
     CDS             49248..50192
                     /locus_tag="PK634_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96495.1"
                     /translation="MANPENNMRLVDPAVALQEIADAVPTACRKHVIIIGSLAAGYYY
                     RDQLKGMAVRTKDADCLLSPRVAALKAGIAIAEELRDAGWEFKKTKGHKVPGTEDTPD
                     AELPAVRLSPPGKIEWFIELLTVPESPGDRGPRWTRLKTKYGDFVLPSFRFLSLANLD
                     PVATEMGISIARPEMMALANLLEHPEIKTDTMSGGFADRMDIKRSNKDLGRVLAIARL
                     AMGRDEDALQSWPALWKTALQDRFPDEWRVLAGRAGQGLRVLLANEQEQDLEQALYTC
                     ANGLLASKKPTLTQLRITGGRLLQDAIKPLEAEARKRA"
     gene            50185..51699
                     /locus_tag="PK634_01780"
     CDS             50185..51699
                     /locus_tag="PK634_01780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOW96496.1"
                     /translation="MPERLTQQELESYLWGAAKLLRGLVDAGDYKQYIFPLLFFKRLS
                     DVWDEEYAAALAETKDKKYARSMADDRFAIPDGAHWADVRGASRDVGRALLTAFRAIE
                     AANPARLAGVFGNASWTDKAQMPDETLKNLIEHFSQHTLSLAAVPEDELGNGYEYLIK
                     QFADDSGHTAQEFYTNRTLVHLMTQMLEPKAGETIYDPTCGTGGMLISALAEVKRRGG
                     DARTLGLFGQELINITASIARMNLVLHGVEDFEIVAGNTLSGPAFIERDKLRTFNVVL
                     ANPPYSIKKWNREAWASDPWGRNFLGTPPQGRADYAFFQHILKSLDPKTGRCAILFPH
                     GVLFRNEEAEMRRKLVELDLLECVLGLGPNLFYNSPMEACVVVCRSRKPPARRGKVLL
                     IDAVNEVARERAQSFLKPEHQARILAAYKAFTDEPGFAKVAATKEILEKDGNLSIPRY
                     VRPVVNGNGNGGGDARSLKKAWATFEASGREFWEQMDELVETLDGVVAGEATDA"
     gene            51692..52870
                     /locus_tag="PK634_01785"
     CDS             51692..52870
                     /locus_tag="PK634_01785"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOW96497.1"
                     /translation="MPEKVKKGWTRVAFGDVVQLCRERAGDPGEGGFDRYVGLEHLEP
                     GDLKIRRWGNVADGTTFTNVFRAGQVLFGKRRAYQRKVALADFDGVCSGDIYVLEAKG
                     NRLLPELLPFICQTDRFFEHAVGTSAGSLSPRTNWESLADFEFALPPLEEQRRIAEVI
                     QAAARLTERWRAVVHHLASLVSALVDVPNNRELQRLPLVTKDLGELAEITKLAGFEYT
                     KFVKYRPDGEVIAIRPLNIKGGRLVLEDVQTIDRRTSGLLPRSKVKAGDVLITYIGEY
                     VGEVLLIEESEKYHLAPNIARITSHGAIMPRLLERLLRGSDLQRQIASRTTTTATPSL
                     TMSSLRKLRITFPSNVEDQRHLVDQVNAVDSASEEVKSRLVAARGLEKRISAETMNGA
                     "
     gene            52875..55838
                     /locus_tag="PK634_01790"
     CDS             52875..55838
                     /locus_tag="PK634_01790"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOW96498.1"
                     /translation="MPFNESNTVEAFIRDRLARSARIPRPGMVSLPGPVWDEGLGWKY
                     AAPLNVPRQPQDVLVESWVREALIRLNPEIAAQRDRADDVLYKLRAIIMGVRSDGLVK
                     ANEEFAAWLTGERSMPFGKSGEHVTIRLLDFDEVGRNEFVVTTQFTYRAGATEKRPDL
                     VLLANGIPLVVIETKTPVRASQSWLDAAIDIHEDYERNVPELFVPNLLSVATEGKELR
                     YGSIGLPVELWGPWRVEAEMPAPALQAVSKAVDSLLRPHVLLDLLANFTCYATDKKKR
                     RIKIVARYQQYDGVNKIVERVVAGQPKKGLIWHFQGSGKSLLMLFAARKLRLHPALKN
                     PTVIIVVDRIDLDTQISSTFYAADTPNLIKAESREELQRLLSQDVRKIIITTIFKFGE
                     ADGVLNPRSNIIAMVDEAHRTQEGDLGRKMRQSLPNAFLFGLTGTPINRADRNTFYAF
                     GADEDKAGYMSRYGFEESIRDGATKPLHFEPRLLELHIDREAIDEAYRELTGGLSDLD
                     RDRLAQQAARMAVLVKSPERIERICADIARHFQEKVAPNGFGAQVVTFDRESCVLYKK
                     ALDQHLPPEASDIVMTVNSGETEYAAYDRDRDAEEKLLDRFRDPADPLKVLIVTSKLL
                     TGFDAPILQAMYLDKPLRDHTLLQAICRTNRPYGEAKTHGLIVDYLGVFDDVAQAIQF
                     DEEGITRVVKNINELKAQLPPAVQKCLSYFAGVDRTVTGYEGLIAAQNCLPNNNTRDR
                     FAADFSYLSRLWEAISPDPGLNPYEDDYRWLSQVYDSVKPSSGSGRLLWHALGAKTIE
                     LIHQHVHIDAIRDDLETLVLDADLLEAVLGTPDPNNKAKEIEIKVSRWLRKRLHDPRF
                     KALGQRLEELKERHEQGLLTSVEFLKELLNLAREVVTAERAKPPVEREIQGKAALTEL
                     FEEVKNPDTPIIVERVVNDIDEIVRYIRFDGWQNTHAGEREVKMALRKTLFKYRLHQD
                     QELFDRAYGYIREYY"
     gene            56106..56951
                     /locus_tag="PK634_01795"
     CDS             56106..56951
                     /locus_tag="PK634_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96499.1"
                     /translation="MSSFPKLQLDPPTDWPSFEDLCWGLWKTIWRDENTQKNGRGSQP
                     QHGVDVFGRPGKASTWAGVQCKGKEVFTHQALTPKEIGREVKKARKFKPPLSEFMIAT
                     TAPRDASLQELARKVTARHAKRHWFSLTVLGWHDIVELLLEYEPPIASRFYPLVFGEG
                     VKGVLWKIEQRLTESPDRDSIAADVPYYAADDSPCHSQDLLQTSQDSTITVSVTMEQP
                     NPGNPIRARDALKALSLLAQIQEICLAQADKNGATSFDATLTSAIDQAKARRNSRDKA
                     DGGAQ"
     gene            56948..60583
                     /locus_tag="PK634_01800"
     CDS             56948..60583
                     /locus_tag="PK634_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96500.1"
                     /translation="MNAKPTSGVTLMDVPILLTRVRHELTAGNYSAAVTLATEIEAVL
                     QVNESSLQKVAADVLLLLAQAHVTQAETAPQDSTPHIKKANELIGKAERMLEQGADTI
                     DKDIALRAAIAAVEEGAEAGLLLLQGRADPFAIRTRTALLLNQQKYSDAMATVEGSAP
                     HEWWCDVAVMAYALNDKVEKAQELVRWAAGLSDRSRYLQCCVRLAEGILGQVVQAHPK
                     DVNILSQTLNAPEREKLVVAVEALGPILQPIQAARKPSSGLDMSALRIAWQANYLLQN
                     REAAAEALKLMAQWTPVPLDVARGGMTGYIEAPADLPDRLRRDHPRDLDANILAAIIE
                     SASFGRHKVAFAQVKDLVPLADTDAKKEELFKLLQTLWQSLEGPEAAECETIAKPLVT
                     HNPRLRTIFEASVALRRGDADTAVALLDGQKATDDLSWLQMRANVCLQKRQFADAVDF
                     LLPAAEKTLDPGLLHKTGDFAFQAKRYDTAAWCYEKLVERQPNNLMVRGNLAHIYTFV
                     LHDLEKAAGQFQALRALEPNNPTHTLNLAVCLAQLFRPEESLALYDELCRLENPPLPT
                     VMGRAQLHHSMGHPDAALKAMDPFRSRFWGESTFLLAYMGTAYAAGREDAAHEALVAL
                     NEMQQAGAVKPEAFRAVPKDEALENFKQSFKQAKDRNEYLHAEMLKGRMPWVWADQVA
                     NNAIYWGWRIRTQKMDWIGDEPTNRARYCIYATNGFHARESERGRRELLPLECPPAGT
                     RIITDISALITLHRLDLLDTVINHFGEMLVPAGYLPTVLEDSRRMVLNQRTQQQNAEQ
                     ITKQINAGRVTVLAEHENPAAGMLVVDEYGETTEHRYRLTDVIQPLYAAGIVTDANLD
                     RISRVSAKPTAVDDSHPALSRFQDVLVDLTTLETITNAGLLDAVASFYGLRITAAAHR
                     EVIQRLNAIACQEETRQWHMDLWKRLRDDPRFKFVPHTVPKAMRDKDSKERDYLPFLA
                     SFIAQETKTPLLVDDRACQAFTLNESADILCAAFGSDALVLRLMAEGKLDAGKTADAL
                     KQLLAWRYRFLVLPPAILKILADMHRGKPPGQALREVAGYVHDCMRDSGLFGGPEKTE
                     LGDSMAMRTYLTWVSSIAEFLVLVWADEGFAPEKATRLTTWACAELLPAPPRVVDGRM
                     KVQIAEMTPRLFLSHALLKTANHSGEPRMADAMKAMKDGLRLTDDEYHRVVLEILNDT
                     AGTAPQP"
BASE COUNT        10629 a        19946 c        19982 g        10044 t
ORIGIN      
        1 gggcgcgcgg cgagtaccgg gatggcgaga tctatttcaa ggcggtcagc ggcaaggaag
       61 gggaatcccc atgagcgcga cggaacaggg cgcggacccg tatttcggcc ggatgaacga
      121 tccgacggcc tcggcccggc tgcggggccc gtgcggcgac gagatggagt tctacctggt
      181 gatcgccggg ggaaagatcg aggacgtccg ctattacacg gacggctgcg cccacacgcg
      241 gctgtgcggc cgcgcggtgg cgcagcgggc tttgggaaag accgcgcacg aggcgctcgg
      301 catcaacccg cgcgagatca tcgagacggc ggaatgtctg ccggacgagg gaaaccattg
      361 cgcgatcctg gccgtcagca cgctgtaccg ggcgctcgcg gcctacctgc tcgaaccctg
      421 acgatcccca ccatgttgaa ggctctccat ctctgcatga accacggccg gcgcgtgaag
      481 cggtatcgcc atgtgccttt cgcgcccggg ctgaacctgc tgatcgggcc caacgggacg
      541 ggcaagagca cgattctcca ggccgtctcg gagtgcccgc attgccggaa gatcgaggag
      601 gggccgacgg aatacgtgct gttcgactcg gagcagatga acccgcgcgt gtccgaccgg
      661 ccggcgcgca acgcgacgga catggtgttg cgggcgcggg cgctgttttc ctcgcacggc
      721 gaaatcctgc aggccgcgtt ctcgaccctg cggctgtcgc cggaaacctg cctgctgctc
      781 gacgagccgg agtccggcca ggacttcgag cacgtcctcg ccctgcgcgc ggcgatggac
      841 cgcaccgtgg cgcggggcgt tcaggtgatc tgttccacgc accaggtgct cttctgggac
      901 cgcgcgcact tcatcgagct gcgccgcggc taccgggact gcgtcatggg ctccatccgc
      961 cgggtccacg gccgggagtc cacatgaaga cgaccttgga ttgcctgccc tgcttcgcgc
     1021 gccaggcggc ggaagtcgtc acgatgtccg tggacgaccc ggcccgccgc gaagcgctgc
     1081 tccggcgcct gctgcgcgag atcgcgggga ccgactggca gggctcgccg ccggaaatgg
     1141 cgcagcggct ccaccggatc atccgccggg agacgggcat ccccgatccc taccgccggg
     1201 tgaaggacga gatgaaccgc gcggcgctag acctcctgcc ccgcctgcgg gatcaccttg
     1261 cgcgtcagcc cgatccgcgc gaggcggcgg tgcggttggc catcggcgga aacctgctcg
     1321 acgccggagc caagacgcag atcggagccg aagacctgcc gcggcacctg gagaccatct
     1381 ggccgcggcc cctgcgcggc cgcgtggacg acctgttccg ggccgcggaa caggcgcgct
     1441 gcatcctcta cctcgccgac aacgcgggcg agatcgtgtt cgaccgcgtg ctgatcgagg
     1501 tcctgccggc ggaaaggatc accgtcttcg tccgcggcgc tccggtgatc aacgacgcca
     1561 cgatggacga cgccgtcacg gcgggcctgc cggaagtcgc gccggtgctc gacaacggct
     1621 ccgacgcgcc gggcaccgtg ctggcggatt gctcgccggc cttccgggaa tggttcgacc
     1681 gtgccgacct ggtgatcgcg aaaggccagg gcaactacga gacgttgtcc gacgtcccga
     1741 agcggatatt tttcttgttc acggtcaagt gtccggttgt cgcggcgcac atcggcgagc
     1801 ccgtgggcag ccttgtggtc aaggagaggc tggcccatga gtagctttac cgtggccatc
     1861 gccagcggca agggcgggac gggcaagacg accgtcgccg tcaacctggc cgccctgctg
     1921 gcggagcggg gcgtggcggt ccagtacctg gacggcgacg tggaggagcc caacggccac
     1981 ctgtttctca agcccgcgct ggaaacaagc gaagcggttg gcctcccggt gccggtcatc
     2041 gacgcggata agtgcacggc gtgcggccgc tgcgccgagg tctgcgagtt taacgccctg
     2101 gtcgtgctga agcgggcgat ggtctttccc gagctgtgcc acgggtgcgg gggctgcgtc
     2161 acggcatgtc cggccggcgc gatccgggag gagccgcgag ccgtcggcgt ggtcgagacc
     2221 ggccgcgccg gggccatcct ttttgcgcag ggccggatga acgtcggcga ggccatggcc
     2281 ccgccggtga tccgcgccct caagcgcgcg cgggcggacg gcgcgctggt cctgctcgac
     2341 acgccgccgg ggacggcctg cccggtcgtc gccgcggcgc gcgacgcgga ctacgtcctg
     2401 ctcgtcacgg aaccgacgcc gttcgggctg cacgacctgc ggctggcggt ggaactgatg
     2461 cgccgtctgg agcggccgtt cggcgtcgtg atcaaccgcg ccgggtccgg cgacgacgcg
     2521 gtgaaccgct tctgccggga agcgcacatc cccgtgctgg cggaaatccc ggacgaccgg
     2581 gcggcggcgg aggcgtactc gcgcggcgaa ttgatgaacg gcctcccggg ctttcaacaa
     2641 gccttggacc ggctgtctga gcgcatcctg agcgagatga gcgagaaggt ccgagcatga
     2701 aggaactcgt cgtcatcagc ggcaagggcg ggacgggcaa gacgagcgtc acggcgtctt
     2761 tcgccgcgct cgccggccgc gcggtgctcg cggattgcga cgtggacgcg gcggacctgc
     2821 acctgctgct ccggccggac atccgccgtc gcgaatcctt ccacgccggg cacgcggccg
     2881 tgatccgacg ggcatcctgc accggttgcg gcgcgtgcgc cgcggcctgc cggttcgacg
     2941 cgatccgcgc ggcggacggc gggaccttcc gcgtggacgc ggccgggtgc gagggctgcg
     3001 gggtgtgcgt ccgggtctgc ccggcgcaag ccgtggattt tccggagcgc gtcgcgggcg
     3061 agtggtacgt ctcggacacg cggcacggcc cgatggtcca cgcccgcctg gagccgggcg
     3121 gcgagaactc cggtaaactg gtcgcgcggg tccgcgagga ggcccgcgcg gtcggccggg
     3181 cggccgggta tgactggctg ctcgtggacg ggccgccggg catcggctgc ccggtgatcg
     3241 cctcgctgac gggcgcctcg cgcgtgctcg tcgtaaccga gccgacgctt tccggggccc
     3301 acgacctgga gcgcgtgctc ggcttggcgc gccatttcaa cgtcccagcc ctcgtgtgcg
     3361 taaacaagtg ggacatcaat cccgcgcaga cggaggcgat cgagcggcgg gtctccgcgc
     3421 tcggcgcgcg cctcgcgggg cgcatccgct acgaccgcgc agcgacgcaa gctttactgg
     3481 cgggccggtc catcgtggaa ggcccgcccg gcgaggccgc cgccgacctg cgggccctgt
     3541 ggaacgccgt caccaacgat gtacggaagg aacgataacc aacagaagga gcacacgatg
     3601 aagatagcca taccggttgc gggcggaaaa ctctgcgccc acttcggcca ctgcgaacag
     3661 ttcgccctgg tcgaggcgga cgaaaagacg cggaaaatcg cggggaccgc gttgcgggat
     3721 cccccgcccc acgagccggg cgtcctgccc cgctggctgc acgagcaggg cgcgaacgta
     3781 atcctcgccg gcgggatggg cgtgcgcgcg cagcagctct tcgcgcagaa cggcatccgg
     3841 gtcatcgtcg gcgcgccgtg cgccgagccc gaggccctcg ccgccgcctt ccttgccgga
     3901 acgcttcaaa gcggcgataa tctttgcgac cattgagccg gccatgaacg aagcgaaaca
     3961 ggcggcaacc ctgctggacc gggagatcgt ccaggtcgtc gggtgcacgg agcccgcgag
     4021 ctgcgcctac gccttccgtg cgatcgcccg gcactggccg ggcccgctgg atctgaagac
     4081 cctccgcgcg cgtctgtggc tttcccacga cgtgcaccgc aacgcctcaa ccgccgtggt
     4141 gccgtttcta catcggcgcg gcgtgcgcgc ggcggcggcc gcgggcctgg ccaccgacgc
     4201 ggcggggttc aacgtgttcc cgtgcatgaa cctcgcgaaa gcacgaaccc tcctgcgccg
     4261 gcgtggctgg ttgaagatca tccggcggcg ccagcggggc atctgggtcc gcgcggaact
     4321 cgtcgcgggg ggccaccggg gcaaggccct gattacagag cggcacgacc gcatcacgcg
     4381 cctcgtcgtg gacggccgcg agatcaggat gaagtacccg ccggccgtcg tcccgcctgg
     4441 cctggacgag atcgtccgct tggcgcgccg gcgcgcgccg gcgctggagg ccgtcgcgga
     4501 ggatttcctg ctcaagcagg ccctcggcca gccccggctg cccgttgaaa aacgcgtggc
     4561 gcggctggtc cgggagcgga tgtgcggata ccggcacgcg atcatgacga tcacgggctc
     4621 gggaaaccac ggcatttttc tcggcctgcc gctgcgcgaa ctataccgcg agcggggccg
     4681 cgcgacgctc ccggccatcg tgctggcgct gctggtccag cttcgcatga gccacgagcg
     4741 gagccgcatc tcggacgact gcggcctggc cctcaaggcc gcgcccgcac ttgcggcggg
     4801 cctggcgtat gcgcggggcg cggacgcgcc ggccctgcgg cgggtcatcc gagccgtcac
     4861 gcgcgacctg cggagcctcg aatgccgcgg cgcgcgagcg agttgcggtg gcaaggcgga
     4921 aaaggccttg cgcgtcgtcc ggaagcatgt cgaggccctt gcatgaacga cgcggtgcgc
     4981 atcaccgtgc tggtcgagaa cggtgccgga gcgccgaacc tcgccgccga acacgggctc
     5041 tcgctctgga tcgaaacccc ggcggggccg gtgctgttcg acacgggcca gggcccggcc
     5101 ctgccgtcca acgcggcagc gctgaacatt cccgtggccg atgcccgcgc cgtggtcttg
     5161 agccacgggc actacgatca taccggcggc ctggcctggt tcctggagca gaaccgcagc
     5221 gcctgcgtcg tgctcgaccc ggaggccggg aaggagcggt acagcctgcg ggacggcgtc
     5281 cgcgccatcg gcatgccggc gccggcccgc gcggcgctgg cggcggcggg cggccgcgtc
     5341 acctggtccg cgcaaccggt ggaagtcgcg ccgaacgtct ggaccaccgg cgagatcccg
     5401 cgggcgacgg actacgagga tgtcggcggc ccgttcttcc tggacgccgg gggaaagacg
     5461 cccgaccggc tgcccgacga ccaggccctg tggacggagt gcgcccgcgg cctggtcgtc
     5521 gtgctcggct gcgcgcactc gggcgtggtc aatacgctgg agtacattgc caagctgacc
     5581 gggacccggc agttccacgc cgtgctcggc gggatgcacc tggccgcggc ttcacaggaa
     5641 cgcctcgccg ccacggccgc cgcgctgacc cgttacgacg tggccgtgct cgggcccggc
     5701 cactgcacgg gcgacgcggc ggcggcgtac ctggcggagc acaccgcctg ccgcctgtgg
     5761 ccttgccacg cggggaaggt cctgacgctg tagcgggccc cgccgcgcat gcggcgtgat
     5821 cgccggcgcc cgcgcaatcc cggacacacg cgcaggaagg gcgggtcgcg acccgcgctt
     5881 gacgatccgg cggcggcgga ctagagacgc tccgggagta tgcaatgaat agactcacgg
     5941 aactgtgtcg cctcgttgcg ctggccgccg caatttccgg cctggtttcc tgcggtaaaa
     6001 aggccgagcc cccgccgccc gcacagacgg aagaggccgt cgccccaacc cccgaggttg
     6061 aagacggccc cgtccgcacg gccttccgcg gcgagacgcc gctgctggac atcgcggtcg
     6121 tcccgcccgc ctccaccaac gaggagccgc tgacgctggt ggccgccggc gagtcgcgca
     6181 ccgtcctgcg ctccacggac aacggcgaga cgtgggcgcc ggtcctgccc gcggagaagg
     6241 acggcccgga tttctcggcc gtgctgttcc cgaccaccgc ggacggctgg ctgctgttcc
     6301 gcgagcaggt cctgcattcc tccgaccgcg gcgcgacgtg gacggccgcc gcggtgccgg
     6361 agcgcggctt ctactatttc ggctcgctct cggcggtcgg aagccagtgc tacgcgatcc
     6421 agccgccgac gtgcggggcc aaggtcttcc gcaccggcga cggcggcgcg acatggacgg
     6481 cgctcgaggg cggcctcccg cgcaacgact acggcgccgt gtttttccgc gatgaccgcc
     6541 gcggctgggt ggtcggcccg tacggccgct atgcgttcac ggaggacggc ggcgcgacgt
     6601 ggaagacgaa agattttccc agcgagatcg gttgggccga actggaaatg gtcacggacg
     6661 atttcgggtg gatgcgcgcc gaccgcggac acgcggggcg gctctggatc acccgcgacg
     6721 gcggttcgga ttgggagacc gtagacctca gcatccaggg atactggaac atcgtggacg
     6781 tggatttcct cgacccggac acggggctgg cgctcgcgca gaagggcgcg gacggctcgc
     6841 tggtcctgct ctcccgcgac gcgggcgcga cgtgggcggt gtggatggac gtgaagccgg
     6901 tgctgacggc catggccttc cgcgacctcg atcacggctg gttcacgagc gccgacggcc
     6961 ggatctacaa gaccgtcccc gcgccctgac ccgccgcggc cgaagaaaca aggtcaggcc
     7021 tttttccggt cgagcacttc gcgaaccttg gcgttgagcg tccggcccgt gaagggcttc
     7081 tgcaggaact ggatgccctc gtccagcacg ccgtgctggc cgatgacatc cgcggtataa
     7141 ccggatatgt acagcgcctt catgtccggc cgcgtttcgg ccagcttgtc cctcaaatcc
     7201 ttcccgttca tcgtcggcat gacgacgtcc gtgatgagca ggtggatacg acccgggtgc
     7261 ccggccgcca acgccatggc ctcgcccggc gcgcgcgcgg cgaggaccgt gtagccggct
     7321 ttcttcagga ttttctctcc cagcttcagg atcggcagct cgtcctcgac cagcaggatc
     7381 gtttcggtgc cgccggccga tccttccgcg gacaccgcct cctcttccgc cgccgatcgt
     7441 tcgcggggca ggctgatccg gaaggtggcc ccttggcccg gccggctggc cacctcgatc
     7501 cagccccggt tctgcttcac gatcccgaac accgtggaca ggcccaggcc cgtgcccttg
     7561 cccgcctcct tggtggtgaa gaagggctcg aagatgtgct ccatcgtcgc cttgtccatc
     7621 ccggtgccgg tgtcgcgcac cgtgagcatg acgaattccc ccggatagga gccgggatgg
     7681 tgccggacat cgctctcctc gacgaccacg ttctccgtgc gaatggtcag catgccggcg
     7741 cctttcatgg cgtcgcgcgc attgaccgag aggttggcca gcacctggtc catctgggcg
     7801 gggtccattt tcaccggcca cacgtccggg ccgggctccc agaccagttc gatatcctcg
     7861 ccgattaggc ggcggagcat cttgagcatg ccggcgacgg tctcgttgac gtccagcacg
     7921 cggggcgtga tggtctgctt gcgggcgaag gccagcaact gccgggtcag gtccgccgat
     7981 cgccgcgccg cggtcaggat ctcctgcagg ttttcgagca gcggctgccc gggttcgatc
     8041 gattccatgg ccatctcggc gtggccgatg atgacgccca gcatgttgtt gaaatcgtgc
     8101 gagacgccgc cggcgagccg gccgatggcc tcgatcttct ggatctggcg gacctgctcc
     8161 tccagcacgc tctgctcggt gatgtcccgc gcggtggaga tcgccccgat cacctcgccc
     8221 cggtcgttct tgagcccgcg gcaccaccag gccagcagcc gcttctggcc gtcctgccgg
     8281 cgctgccagc tttccacgta gaccacctcg cgggagcctt cgaacacggg tttcaccagg
     8341 tcctcggtgg attgctcgcc cgcgaaatag gaggacgccg accggccgac gacgtccgcg
     8401 ccgaagaacg ccaggccagc cgggttggcc cacgtataga cctggcgggc gtccacttcc
     8461 atgacgatgt ccgggatggc ggacagcagg gcctcctggc gaaaggacag ggccgtcagc
     8521 gaggcctgcg tgcgcttctg gtcctccacg acgctcaaga gcgccttgcg cgatttgcgc
     8581 gcggcctcga gcaggcgttg catctccgcc tccgtcgcgc gccgctgctc gtcgacccgc
     8641 ttccactcgg tgatgtcctc ggagatgccc agcatgtact cgggttcccc ccgctcgttg
     8701 aggaccggta ccttgcgggt gtggagtatc cgcgcgccct tgcggcgcgt ctgcaaccgt
     8761 tcctccggga tatcgagagg cttcctcccc tccaggactt cccggtcctt ggcgatgaag
     8821 aagtcggcct gttccttcgg gaagatatcg cggtcgctcc ggccgatgag ttcctcccgg
     8881 gttgtgccca acaggtcctc ccccgcgcgg ttgaagcgga cgaaccgcag ttcgcgggcg
     8941 tccttgatga agatcatgtt cggaatattc tcgatgatcg agtcgagaaa gcggtgcgat
     9001 tccgccacca gatcgaggag gcggttgata tggtccgcga cgccggccag ttcgctcggt
     9061 cccctccgcg gcacccgccg cgtgaagccc ggcgaggcgg acacctcatc gatatcccgc
     9121 cgcaagttcg ccagcggccg cgagacaagc ctcctcaaca agacgacgat ggcgctgata
     9181 aaaacgaggg ccaccaggcc cagcatgagc cggccgaacc gggcgatgcg cagccccacg
     9241 gcgtatatca tgcgcggccg gcggatctcg atgaccgccg ccggttgctt ttccaggtcg
     9301 cgcaggagcg cgtaggcgcg catcgtgttc tcgtccagcg gcaggatggc ggccggggcg
     9361 ccgcgcagca gcgcgggcca cgcgccgctt tccgtgacgt gcgagggaag ggctcgcgcg
     9421 gggtagacgt tcacctgcag atggaggatg gccgccagtt ccccgaacca ggcggcatcg
     9481 agggcgcgtc ccatcaccac cgtcccgcgc ggcgggcctt cccgctggct ggtcaggatc
     9541 ggacgggccg ccacgaccag gggctccccc gccaggctca tgagccccgc caggctccaa
     9601 tcgccctcct ccccgcgggc caggggcgcg ccaggcgcca gccagcgcgc cgcgtcgtcc
     9661 ggcagcgggc gcaccgcgct gctttccgga tccctaatcc cgccccagac cacggcgcca
     9721 gacgggtcca cgatgatcag gagccggatg ccgagcgact ggaacgtgtc cagtgtcagg
     9781 ttggattcga tgaaggcttc gttccggtcc tccacgaacg cgtaggtgtc gtcccagaac
     9841 gcccagtccg cgaccgagcg gtccaggtgg cggccctccg cctcgacggc ccattggacc
     9901 tgctggacat tctgcagcgc aaaacgctgc tccaggtcgg cgaatccggc gcgaagcccg
     9961 tgggtgccca ctgcgtacaa cacgatgaac agcacggcca gggacgggcc cagcgccagc
    10021 agtactcgcg aacccagttt catcgtcgct ctctcctcgc catccgggcg ccccgttact
    10081 cctcgccgtc ggccgcgccg tagcggggcg gttcacccag ccgggccagc agctcgttca
    10141 cctcgttctt cagctcgatc acccgcattt cacggcccat cgtgccgtcg tgccagcggc
    10201 gcaactcgtc cagctggtcg cgcacggccg cctccatcct tttctgatcg gtcacgtcgc
    10261 ggaccaggcc gccgataccg gtcttcccgc cctccagcgg aacggggaac ttccgcgtct
    10321 cgtacatgcg cccgttgcgc tcgtcgtggt cggtcgcggg gcggccggtc tccagcacct
    10381 gccggtcgct cgcgcgccag cgatccgccg cctcccgggg cgagagctcg taatcggtac
    10441 ggcccaggat ggcctcgacc ggccgcccgt gaaacgccgc gtaggcgtcg ttcacgtacc
    10501 agagccggaa ggcttcatcc ttgatgaagg ccatgtccgc gatggcgtcc aggaacaggc
    10561 ggctgcgctc ctcgctggac cgcagccggg cccgcgcgtc gttgagctcc aggaccttct
    10621 tgtccagctt ccgggcgatg gtcgcgatgt actgggcatc caggcgatcc ttgtcccggg
    10681 ccgacggacg ccggacaggc gccgtgcgga cgcccgaggc caggacccgg cggatctgct
    10741 ccataaacgc gtcctggtcc atgggcttca cgagaaacag gtccgcgccg aactcccggg
    10801 cgagttgctg atcctcctcc gtggtgaacg tggccgagta gatcacaagc ggtatgcggg
    10861 cgaggacggg gtcgctcttg agcgtccggc acaaggcata gccgtccatg tccggcatca
    10921 tgatgtcgga aacgatcagg tccgggatcg cctccttcag ccgctccagg gcctcgcggc
    10981 cgttccgggc gctgacgacc tcgtagccgt tgccctccag gatcgtgtgc tgcagcaccc
    11041 gcgagttctc gtcgtcttcc accacgagta ttttcatgac agttctccca cgatgcgcgt
    11101 gatctgaccg atgacccgtt ccggatcgat gggcttctcg atgtacccgt tgcagccggc
    11161 cgccagcagc cgctgctcgt cgccggccat ggcataagag gtcatggcga tgatcgggat
    11221 atggcgcccg gcctcctcgg cccggatgcg cttcaacacc tccgtgccca ggatgtccgg
    11281 aagctggatg tccaggacga tgaaatccgg ccgctcccgc agggccttct ccacgccctc
    11341 ccgcccgcac acggccttga ggaccgagca gccgtgcgtc tccaggatga acgtgatcag
    11401 ctccatgttg ttctcgttgt cctcgatgac cagcacgcgt ttcatgacgg cttctcctcc
    11461 cccggttggg ccggccgccg ccgcggcgcc cgcagaatga aagtgctgcc ccggttcttc
    11521 tcgctccgga cctcgatctc gccgagcagg atctcccggg cgatcttgcg ggtcaggtac
    11581 agcccgaggc ccgtcccgcc ggccttgatc cgcaggtgcg aatccagccg cacgaacggt
    11641 tcgaacagct tggcctgctc ttccggcgag atgccgatgc ccgtgtcgct gaccgcgacc
    11701 tcaaatcgct cgctcaggtc ccgcgcctcc acccgcacgg agcccgcctc ggtatacttc
    11761 accgcgttgc tcaagtagtt cagcaggcac tgccgcagcc gcttgcggtc cgtgaacacc
    11821 tccagctcgg gcagcggccc gcactccacg cgcagggact tcacctccgc ctgcggccgg
    11881 atggactcca ccgcctcccg gatgacctcg tccagccgga acacctgggg atagacgtcc
    11941 acgcgcccgg cctcgatctt ggagatgtcg atgatgtccg tgatcagggc cagcaggtgc
    12001 tgcgcggaat ggtagacccg ctgcagctgg tcgcgctgtt tatcgttcag ctcgccggcc
    12061 atgcccttca gaattacgcc ggtgaagccg atgatggaat tcagcggcgt gcgcagttcg
    12121 tgcgagacgg aggcgatgaa caggctcttg agctggtcga ggtcctgcag ctgccggttg
    12181 gcccgctcca gctccacggc gtagcgttcc aggttctcct cgatctgctt ccgccgcagg
    12241 gcgctggaga gcatttcgcc cgtgagccgc agcaggctga tatcctcgtc cgaccacggc
    12301 ggcgcctccg gccgcgtgtc caggagcccc aggaagccgg tcacctgctc ctccgtcgcg
    12361 cacggcacct caaccacgcg gccgcggatg ggccggacca ggtgctcctc cccgtccgcc
    12421 cgggccgaga ccgtcacggg gcggccggcg cgcaggcgcg ctagccactc ctcgtcccgg
    12481 tcgcccggct ccgccgcgcg gagggcctcc agatcgccgg ccgcgtgggt ccgctccgcg
    12541 cgctcctgcg gcccgaaggc gtacaggtag ccggcggcgg tcccggtgaa gcaggcgatc
    12601 tccgccagcg cgacctggat atgccggtcc atctccgccg gcttgacagc gacgagccgc
    12661 gacgaagtct ccgccacgag ccgctcgaag cgctcgcggt agttcagccg ccgctcgatc
    12721 tcccgccgcc gcccgatctc cttcttcagg cggaggttcc agaagaggat caccagtagc
    12781 accacggcca ccccgaccgc gatccgcagg ccgatgcgcc acaccacgcg gcgctcgatc
    12841 cccggcttgt actccaccgc aacccaccgc tgctcgatct cgcgctgctg gtccgcggtg
    12901 accaggtcca gggccttgtt caggatgccg agcagttccg gccagtcctt gcgcacggcg
    12961 aagtgcagcg tgtattgctc cggcgaggcg ggcgcggcca ccttgagatt gatcaggttc
    13021 aacttgcgga tccagtacgc cgccgacgcc aggttggcca ccacggcgtc caccttgccg
    13081 ccggagagcg ccagcaggct ctcctccagc gagccgaaca gcacgggttc ggccgggctg
    13141 ttctcccgca ggaaaccctc atgcgacgtg ccgctctgca cgccgacgcg cagcgattcg
    13201 aggtccttga gcccggccag gaacggcatg tcgagccggg tcatgatcac gcgctggaag
    13261 cggatgtagg gctgggagaa ccggagccac tgctcgcgct ccagggttcg gccgacgcag
    13321 gggagcacgt cgatcgtccc gctccggacg ccggccacgg ccgccgtcca ggccaggccc
    13381 tcgaccaggc gcaggttcag gcccagccgc tcgttgaaca ggcgcacata gtccgacgcg
    13441 atcccctggt actcgccccg cggcccgcgg aactcaaacg ggtagaattc gggatcgatc
    13501 ccgacgcgga tcaccggatg ccggcgcagc cagttcttct ccccgtccgt caacaccgcc
    13561 gtccaatccg cggccgcggc gggcgtctcg ccgtgcggcg ccccgtagag atgcgtatcc
    13621 atggcgcggt aatagaacga ctgcgggtcg accttgagct cgcgcaacct cctgtcgagc
    13681 gcgactttga ggcgcgcgcc ctgcgacgag cccttgggga aggcgaaccg cagctgcgac
    13741 gggctgaaca gcaccggcga gcgcgccacg ttgtattcct tctcgaagta cgagccgaag
    13801 atgttgttca ccacgcccgc gtccgcctcg cgccggctca ccgcctggaa gacgtcccgg
    13861 taggattcga actccacgta ctccgcgcgg atgtcgaact tcgcgagcag ctccggcagg
    13921 ctgcccgtgc cgctcgtgta tacgctgccc ctcatgaggg cgatgcgccg gtccgccagg
    13981 tccaggaacg actgcacgac cacgtccggc cgcgtgtaga tcaccgccca gctgatcagc
    14041 gccgtctcct cggtgaactc gaacttctgc tggcgctccg gcgtcagggc cacgtccggc
    14101 atcacgtcga tctcgccgcg ctccaggcgg tccaggcact ccttccacgt gcccttgcgg
    14161 tactcgatcg tccagccttc ctggccggcg atgtgttcga ggatggcggg gaaaaacccg
    14221 ctgacgcggc cgtcgggcag cgagtagatc ttgggcaggt tctcgtaggc gccgacccgg
    14281 acggcctccg cggcgcggcc cggcccggcg gcggccagca gaaggccggc cgcgaccacc
    14341 ccccgcgcca ccgtgtttcc gttacgcttc attcggagag acccggcggt taacgaccgc
    14401 ctcttcgcgg tttattatgg gcttcccgct tccttcgcgc aagcttttcc ccttcctcat
    14461 cccgctccgc ggccgcgtca gaagcacagg cgtacgccgg ccatgacggt cagcacgtcc
    14521 tcgtactcct cgcccggccg gtcgagcgtg aaggtccgca ccgacgcgta caggtccgag
    14581 gcgatcggct ccagtcgctg gttcagcacc acggcgcggg attcggcgcg gtcccccgcc
    14641 tgcgagattt cctcgttgcg cgaatagtcc accgccaggc aggtctcgcc gcacgacagc
    14701 cagtccagcc gccagccgat cttgccgtac caggagaacg gggagtaatc gccctcgacc
    14761 tcctgcccgc cggccgcggc ggtgacgttc agcccgccat ccagccacac ggagcaggat
    14821 ccgctgaact ggtccacgtc cgcgcccttg tgtgcgaccg cgagcgccgc ggcgccctgg
    14881 catgggccga ggtcgcgggc aaagcggagg gccgcgtcgt agaaatcctg gcccgcggcg
    14941 gaggcggccg cggaccagcc gtgccaggcg ggcgtgtcgt agcggagccg gtcatcgcgg
    15001 tgcagcccgt cgaagttgtt gaagacctcc ttgatcgccg gcccgttggt tccgaagacc
    15061 aggctcgccg cggcaaaccg cacctggctg tagctgatca cgtaggtgcc gctcaagtcc
    15121 tcctccgccg agccgttcga cgccatgtcg ccctgcccca gcgccaggga accggcggag
    15181 gggctccggg cgtacgcctc gatcttgcgg ccgtccacct cgaactcggg atcctcgccg
    15241 ccgaacgtca cggccgcgga attgtccgac ttgaagccca gctcgacctg cccgccgaac
    15301 gtccagtccg gccagccgtc cggcgtgtac tctcccgccg cgcccaggcg cgacggcgag
    15361 ctgtcgttgt ccacgtgaaa ccactccgag aagcttccgt tctccgcgta cagcagcgcc
    15421 cggttcacct gcccccacag gcggaccttc gccttccgcg tcgcctccac gagcgcccga
    15481 cccgccgccg gctcgttcgt ctgcgcgcgc gcttcgggcc cgcccaggag cgccgccagc
    15541 aggacaacca ggagacctct tcctttcata cgccggccct cccgtcggtt atcccgcgcc
    15601 tcgtcaaacg gtaacaacct ccgttggtta tagtttcgtc gcgggtgcct cccgacgcaa
    15661 gcgatttccg cccggccgca tcgagacgac gcacccggga ttcccaagca atggaaaagg
    15721 tgatcgggac cggatcaggg ctcgcggacg cggagccggt agacgccgcc cgccgacggc
    15781 tcgacgggga cctgcaccgg gccgccggtg ccgggcatat cgttggtcag gctcgccgcc
    15841 caggcctgtt ccgccaggtt cgactcgccg tccaccgcgt acagccggcc cgtggccgta
    15901 tcgaaaagaa gcccctcccc cgccagagag cggatctcga attgcgcgtc ggggtcgtga
    15961 gggtcgagcc cggccacgta ggattgccag accggcattc cgttggagcc ggtcaggagt
    16021 tccgcggcct ccatgtcggt cgtgaagccg tgttccgcca gccacgggta cggtgtgccg
    16081 aagttcgcgg cgcgcacctc ggcgaagacc gcgtggaagg ctccggtgaa aaggacgcta
    16141 ctccacacga actcgtgcac gccggtgccc aggtcggcgc ccgccgcccc gccgttggtc
    16201 cagagcgagt cgagggtata gtacaggtcg ggcgtgatca ggaaggcggc gtcctcgccg
    16261 gaaaggacgt tggtctggag cggcgagacc tgcccgtgcg ggcccgcatc ggcgaccagc
    16321 gggaagcgct cgttcacggt cagctggttc cgaatccgcc aggtctcgtc cgggtccgga
    16381 tcgaccagcg tcaccgtggt ccagggcttc gcggcgtcgt agagcacgtt ggtcaactcg
    16441 gccacggaga tgatcccgtt tgtggcggca aacataaagc cgacggaatt cgaggggccg
    16501 gagagcgtga cattgggctg cccgggcgtg gccacggccg atgaccagtg aacgcttaaa
    16561 gccgggttcc agttgatcct gggatcgttg gcagaacacc cggcgggcgg gccaaacggc
    16621 atggagggcc caccgaccac cagcagaggc gccggtccaa tcacctgaaa acaagtcgcg
    16681 ggccaaccgc gccagacgcg atccacggcg gtcccgaggt actgcgcctg gatggagtcc
    16741 tccagtatca cccgaacgcc gatgaccggc ggtggataga caggctgccc ggggccgttg
    16801 acgggggtgg catggctgaa tgtgaaagtg gacagcttgt agtcgtgccc ctcgtgctgg
    16861 atcgccggcg gcgaggactg aagcgccagg ggattcgtaa tggcaatggg catgacctgg
    16921 caaaccagcg ccccggaata ggccacctgg aattctggat tcgccgggtc ggcgggaaac
    16981 gggaaccacg tttccacggt caggtggaga aaatgttcaa gcagcgtgtt ggtcccggcc
    17041 tgcaccaggc ggaacgtgtt gctgacgatg acctcgttga tcatcgggac gggctcggcg
    17101 ctgattcgat ccagccgctc ggcgtcggtg ccgccgtacg cgaacctatc gctgtccgcg
    17161 taatcgtgca gcgcggcgat gaacgcgttg agatcgggga tgggcgacgg ggccaggtcc
    17221 gcgagggcgg tctgaatttc cgcgagattc cacaggtccg gatcgccgcc gatgtaggcg
    17281 atgttcgtag atgcgcacgc ggcccaggcc agcgctagcc aggccagccc gcttcccgcg
    17341 atgatccggc ccctcctcat gcggaccgcg ccggattact cgtgcgagat ggcgccgacg
    17401 gggcagccgt cctcggcgcg cagggccttc tcctcgtgct ccggcgggac ggggtcgagc
    17461 tggacgttgg acttgtcgtc cacgagggcg aagaccgccg ggcaggtccg ctcgcatgcg
    17521 ccgcacccga tgcacaggtc atgatccact ttgaatttca tggcggggga acacctagca
    17581 gatcggcggc cggttgtcca accccgcggg cggcgcgtca gaccgccagg cagcgggcgg
    17641 tgagttcgcg gaggacattc agctcgaacg gctttcccag gcattcgaag gcgccttccc
    17701 gcttggcctc gtcggtcaat tcgcgcgtgg ccgagccgct gagcatgacg atatgaacgc
    17761 ccggcgcgag ggccttcatc gcgcggatgc cgtcgatccc gtttcgcccc ggcatctcgt
    17821 agtcggtgat gaccagcccg ggcccgagcc ggcggaacag ttgcagcccc tcgtccacgg
    17881 tagccgcgca ctcgacccgc ccgtagcgcc gcagcagccg ggccagcagg tcccggatgc
    17941 aggaattgtc gtcaatgacc agtatcgtcg ccgccgtttc gctccgcccg tcggtattca
    18001 tgactcttgc tcctctgcct gtgtagtcgt cgcaaagggc cctttcttcc cgcccaagag
    18061 agtttcggcc gaaaaaagaa caagccggga agaatcccgc ccccgcgccg actgatacgc
    18121 tcaagaccgc ccgccggttg accaaccccg accgatgaag ggtgccggcg ggaggcggaa
    18181 agacggaaac aggaggttcg tgatgcaaag gctgatgaaa gtgctgacag gcgtcgggct
    18241 ggcctgcatg accgcgacgg gggcgctcgg cgcttctccg cccttcgaag agatctggga
    18301 ggccgcgggc tccatctcgc cggactacaa caagaacacg cacctgttcg ggcacgccgt
    18361 cgcgatgtcc ggcccgcgga tggccgtcgg ccggccgggc gtgcaactcg ggggcgcggt
    18421 gaactgcggc aaggtgcata tctacacgct ggacagcaac gggcgagaag tcgccaacac
    18481 gctgcgcgtg ctggagccgt cgtcggccgc aaacgacgtg ctgagcgggg cgttcttcgg
    18541 cgccgcggtg gacctgaagg gcacgcagtg catcgtgggc gcgccgggca acgtccgcat
    18601 gaccacggcc gcgcaggaca accggaacgt ctacgcgggc gcggtgtaca tctacgaggg
    18661 caacaattgg cagcgcaccg cgcggctgcg gcccgccgcg ggcgccccgc ggacctccaa
    18721 gttcggcgct tccgtcggca ttgacggcga gttcgccgtc gtgggatcgc cgggggaatc
    18781 gtcggtgtac ttctaccgca aggtcaacgg cgcctggaac caggtggcca aggtcaccaa
    18841 ggcgggtttc cggttcggga cctcggtggc catcagcggc acccgcatcc tcgccggcgc
    18901 gcccgaggag gaagccgggc ggggctgcgt ctatgcctac cagtggagca acaaccagtg
    18961 gcagcaggct caacgaatcc gcggcgtgat cgcgggcgcg caattcggcc acagcgtggc
    19021 gatggacggc ctgcgggccg tggtgggctc gatcgccgag aacacctacc ggggcgccgc
    19081 ccgagtctac gagttcagcc agaaccaatg gcagtggaag gcgcgcctcg cctccgagac
    19141 cgacgtggac taccaggcct tcggcgactc ggtggacgtc agcggctcga agatcgtcgt
    19201 gggctccctg gccgacccct gggacgatcc gaccacgggc gaaacctacg tctacggcct
    19261 ggcctacaat gcctggaacg tgctctatta caagcacctg ccgtggcaga acggcgacta
    19321 cgcgtcctac attcacgacg tcgccatcga cggcaacctg gcggccgggt ccgaagccat
    19381 cgacgcggcg tacggcgaca acgacaggaa tctcggccgg gtggacgtcg tgatgttcga
    19441 ccaccacgag gtcttcttcg agaatgtgcg cgaggccacc tacagcttgt acacgccagg
    19501 gtccgtccgg cagggcacgg cggtgctctt caacatcgaa atcacctggg atgcacgggt
    19561 gccggccgcc gaccgcgtgc tctgggtgca attgtgggac gtgcgcggca tgacctcgat
    19621 ctaccaacat tccttctccg ccggcccatc gcttgtggcc aacggtgcag gcacgtcttc
    19681 ggccctggcc ggcaacatgc cggtccggtt ccgcgtctgg cctgcgcggg cgctgcccgc
    19741 cggcgtcccg ggccccaccg tgatcctgca cgcgcagtac accctgcaat aaactcgacg
    19801 ccggataggc gtccacgcgg tgagacttag tacgcggccc gtcccgccct gcgcggggcg
    19861 ggccgctttc cgttttcccg cggacgctgg aacgacgggc cgggactttt ctgtatacac
    19921 cggcaatccg gcaggttatc gtgtcaccgg taggcagcac ccaagccaca gcgcatgagt
    19981 gagatcagcc cagaacagct cggccggctg caatccgggg accgccgggc atgggacgac
    20041 ttttttgcgc agcacgacga actgatccgc tcagtggtct cctggtcgaa gtggcggttt
    20101 tcgccgccga cgagggagga catggcccag ggcatccgcc tggccctttt caaggccatc
    20161 gagtcgttca acgaggaatc ctccgtcgag tactacgtca agcgcatctg catcaaccgc
    20221 tgcatcgacg aggtgcgccg ccagatccgc gcgcggcaga tcctggtgcc gctggccacc
    20281 ggggacgggc agcggccgga ggaggagacc gcccgcatgg ccgaccggtc ccaggatccg
    20341 ctccgcgacg tgatcaacgc cgaggtcgcc ggccaggtga agcgcgtcct cgagcaggtg
    20401 gacgagctct gccgcgtcgc catccagcgg ttctacttcg cccagcaatc ctacaagcag
    20461 atggcggacg aactgggcat ctcgatcaat accgtcggct cccggctggc gaaatgtctc
    20521 gagaaaatgc gcaagatgtt cagcgccgac ctgtttttcg gggaagattt ccgcgtctcg
    20581 aacgacagtg gccagtgaag gagctccgtg acatgagcga gaccgacaaa ttatgggctc
    20641 tggtgcacgg ccaactgaac gacccgcagg ccgccgagct gcgggaggcg ctgaaggccg
    20701 acccggccct gcagcggcac tatcgccaga tccaggacat ggatcgcctg ctgcgggaga
    20761 ccggctcaca ggcggaggtg ccggaggaac tgctggtaaa aaaagtgctg cacgcctggg
    20821 agacttcgcc cgaggcagga cgctggggcg ccgccgcgcc tgacgcttcc gcgccgacca
    20881 gggtgctgcg attcccgggc gaggcgctgc tctggctccg ccagccgtgg cgcgcgctgc
    20941 agaccgcggc caccctcgcg gcctgcgcgc tggtcctgct cggcgcgcgc aactatttct
    21001 cgccgagcct ggcctggcaa ccggaccggc tggaggccgc ccagtaccgc ggcgacaagc
    21061 cggcggacac cggcccggtc gtcgaagccc tccgggccat ggccgctgat ctccgcgcgg
    21121 agatcgcctc ccattacgag cggcaggccg gccgcacggc gtcgcgctcg gtgctgcgga
    21181 aacgcacgga atggcgcctg gccacgcgca tccaggaact gcccgagagc atgttgtccg
    21241 tccaggtgga cgcctttcgg ccgggcgacc cggccccgga gatccgctgg acgcggcagt
    21301 tcgagaacgc ggaggctttc tcgcgggacc tgccggagtt cggcgggcag gtcgcctcgg
    21361 acctgctccg cgcggcccgc cctgcccaac cgtaggagcg gatcatggat gcccggcgct
    21421 tcttccggtc cctagccgcg gcgggactcg cggggctggc cgccgggtgc gcgcccaccc
    21481 cgcccgccgg gaactccgcg ccgcctccgc acaagacctg gtggaacttc tacgcgcggg
    21541 gcgcggccct ctcgtatgcc ggcgactacg cggccgccgc gcagcagttc cgcatctgcc
    21601 tgggactgga acgcggcgcg gtgtacggct acccgcgcga cacgtggcgc gcgcggacgt
    21661 acggcttcca tttcatcgac gactactttc cccaccgcga gctcggcatc tgctatttcc
    21721 agctcggcca ggccagcaac gccgtccgct tcctggagcg ctcgctgcag cagcagcctt
    21781 cgggccgggc ccgccatttc ctgaaccggg cgcgcgccga actggtccgg ggccgggtga
    21841 gcgcgccgcc ccggatcgag gcggactcgc ccccgccccc gtggagccgg gaccggacgc
    21901 tgctcgtctc cgcgcgcgtt tccggcaaag gctatgtgca ggacgtggcg ctcaacggga
    21961 agccgctgtt caacgaactc gcggtccccg tcctgccggt cgagcaggcg gtggacctgg
    22021 cggagggggc caatgtcatc cggttgtccg cccgcgacct ggcgggccgg gaggcccgcg
    22081 ccgagtggac ggtcaacgcc gattggcagc cgccggcgct cgccgtcctc ggcgcggaac
    22141 gccgggcgga cgggtgggcg gtgcgggcgc ggggctcgga ccgcttcggc gtggcccgca
    22201 tcaccgtgga cgggaaaccg gtgacgaccg atggggggac gcagcaggac ttcgaattcg
    22261 tcgcgcccct gtcgtcgccc ctgccggtcc agatcgagga cttcgcgggc aattcgctga
    22321 tccgcctgtt cagcgcggag accctctccc cgctggcgca ggcgcgcgcc ccgcggtacg
    22381 cggcccggca gccggagggc cggccggaca cgccggccgc cgcggccccg gacgagggcg
    22441 atcgcctgcc gcccggcttg cgcctgcata ccccgctgcg cacgcgggtg ttcaacagcg
    22501 agttcctgat cgaggtcacg accacggacc gcggcggcct gaagtcggtg accatcaacg
    22561 gcgaggacca gttgcccgac gcctcgcggg gctgcgagca attccacttt gcccgccgtt
    22621 gcagcctggc cccggggacc aatacgtacg acgtcgtcgt caccgaccgc gccggccgga
    22681 ccacgcggga ggagtgcgtc gtggtgagcc agtcccccga gtacctggac gaggagtacc
    22741 ggctccggct gggcgtgtcc cccgtcagca cggccctggg ggcggaaccc gtggtggacg
    22801 accgggccga gcgggtcggc cggatgatgg agcacgagct gctcctgtac ccgccgcggt
    22861 ttcgcgtgct cgagcgggcc gagggctggg acgcgctgat cgaggagcag cggttgagcg
    22921 catccgacct ggcggacccg cgcgccgcgc tgcgcgtcgg caagctgatg ccggccgaac
    22981 tgctggcgat aacgctgctg atcccgcagg gcggcagcct gaccctgttc agccgcgtcg
    23041 tggacacggc cagcggcgag atcctgtgcg ccgaggacgt ctacgtgccc gatccggacc
    23101 gggacctcgc ggatcgggtc gaggggctgg tcatgaagat cgagcagcaa tttcccctgc
    23161 tggacgggaa gatcacggag gcggccggcg acatagctac cgtggggatc ggcgcgctgc
    23221 acggcctgaa acccgggatg cgcttcctgg tcctggactg gccgggccgg gagcgcggcc
    23281 ccttggaaga gagcgcggtg cggcggtact cggagcaggc ggtcgagctg cgcatatcga
    23341 gggtctacgc caagaaggcc caggcgcgcg tgagcccgcc ggaagccgcg gccggcgtgg
    23401 cggaaggaga cgtgatctat gcccgatgac gcgagagagg agcccgtggg cgtgaaaaga
    23461 accgcggccc tcctgctggc cctcggcctg tgcttcggct tggtcttcta cctcgggcac
    23521 tcggcctccc gctaccgggg agccgccccc gtggaacccg gcccggcggc gcggccgggg
    23581 ccgctggcct tccacgccgt ggtgatcggg gccagcgact actccgcggc cggcggccgg
    23641 ggatgggagg acctccagac cgcccgggcg gatgcggagg cgatcgccga tgtgctccag
    23701 cgccggtatg gattcacggt cgaagtgttg accgggcagc gcgcctcgaa ggcgggcatc
    23761 ctgaccgcgc tggacaggct ggccgacctg accgaccggg acgccgcgct gatctactac
    23821 gcgggccacg ggcattacga cgccacgctg gacgaggggt tctggatccc ggcggacgcg
    23881 cgccggaccg tggacgggcg cgatgcccgg gaggactgga tctggaacag catgatcacc
    23941 aagatggtga acgcctcgcg ggcgcggcat atcctcgtgg tggcggattc ctgctacgcg
    24001 ggctccctgt ttcgcggcga cgggatgccc gcggagaggg agtggacgtg gtacgcccgg
    24061 gcttttcacc ggccgtcccg ctatctcgtc gccagcggcg acctggaacc ggtgctggac
    24121 agcgggatcg agcacagcat gtttgccggg gcggtgctcg agttcctcga gaatcccgtc
    24181 agccccgtgt tctcggcgtc ggacatggcc ctcgcggtgc gccagaaggt cggcgaactg
    24241 accggccaga tggtccgcat gggcccgctg gcggtgtcat cgcatgccgg gggcgagttc
    24301 gtcttcactt ccgacccatc ccggctggaa ctcgcggacg tcgcggccga tccctcggca
    24361 cagcaacgga gcccggaaac agccgagccg cccggcgact tggagaccgc gcgcgatgcc
    24421 ttatggatgc agcaggcggg cctggcccag tccgcccgtc ggctgaccgc ggaggcctcg
    24481 ccggaagcga agcccgtcac cgccgcggtc gcggcttacc tggacccgga acggcgcagg
    24541 ggccggcagg acgccttgcg ggagttgatc gaacaggtcc gcgagcagcc cccgcgcgcc
    24601 gacgcggcgg accgcgtccg gccccgcgtg ctggcctgcc tcgggccgga cccgactccg
    24661 gccggcggcg aggagcaggt catggcccgg ctgatccgga tctgcctgac ggccgccctg
    24721 caggggcaaa cacgcatcgt ggccgtggag cgggagaatc tcgaggccgc cttgcaggag
    24781 cttcaactgg gcgtctccag tctctccgat gcgcaggcgc agttgcaggt cggccgcttt
    24841 ctcccggcca gcatcctgct gctgggggac atggcgcggc aggatggacg ctgggtggcc
    24901 tggttgcgcc tcgtggacac ggaaaccagc cggatcctga aatcctggaa cacggacctg
    24961 gacccggcga acctgcagga gaactgccgg gagatcgcgg cggacatagc gcgggccgtc
    25021 gcggaaatgc gccccctgga ggccgcggtc acccgcgcgg cgcagggcgg ctatcgcgtg
    25081 gccatcggca gtttccacgg gctggatcca caggcggcgc tggacgtgct ggcggacggg
    25141 aagccggcgg gctcggccgc ggtgactcgt ctcgggatca tggaaagcga catcgccatc
    25201 gaattcgcgg gaggcggaaa accggggcag aataccgccc tccgtgtgcg cgagcggcag
    25261 ccctgattat ttccatgccc gcgcgccgcc cagcgggtat aagagcggat ggaggcatca
    25321 tgccgcgaag tcgaatcggg atctgcatgc ttgcgctcgg aagtctgctt ggaacccagg
    25381 cggcccgcgc agaggatttc gtgatcgaga cgccggccgg gcccgtgaca atctcgctgg
    25441 accatgccgg tccgcagggc agcctggtgc aaggcggcgc gccgtcgtcg ccggccttcc
    25501 gcccgccgag catcttctcc gcgccgctgc cgagcggttc cggcgcgcgg gcgctgggtc
    25561 tggccggcgc gttcacggcg gtggccgatg acgcgacggc ggcctcgtgg aacccggccg
    25621 gcctgatcca gttggagcgg cccgaggcgt ccgtcatgct gcgtgcctcc cgcgagcgca
    25681 acgcatacaa ctcggattcc gacgccttcc gggtgggggt caactcttac gacgatgaga
    25741 acctgaacta cttgagctgc gcgtatccgc tcacgcttct caaccgcaac gtcgtgcttt
    25801 cgatcaacta ccaggaagcc tacgatttca accagcgatt cagcgccgcc gccagcgaag
    25861 gccgcccgcc gtcgtccagc gcgtccacgg aaacgcgcgt ggatacgtcc acccaggtgg
    25921 atcattttga aaacgaactc gacgagggcg tcaccgcctc catcacgttc gtcacgcacc
    25981 tgaccacgcg gcacaccagc gccctgcgcg aactgctgca ggccgacctg gtgaacgacc
    26041 ttgaattcga gcaggagggc atcatcgacg cggtcacgcc cgccatggcg gtggagatcc
    26101 tgccgcagct gtccctcggg ctcgcggtga acatgtacca ggacagtgcc ctgcacggca
    26161 tccgttccaa gaccaccgcc tcgtacacgg gttcgtcgac cctggacagc gccgcgcagg
    26221 agacgcgtgt cacctcgtcg acctacgagt acgagggcct catccgctat cccccgccct
    26281 tccccgagat ccccttttcg agctcgggcg agttccccga tttcacagac agctctacat
    26341 ccggggatac ctccggcgtc gtggtggacg gtgtgtacga ggaggaaaat gtcctggacg
    26401 acctgcgcgg cttcaacacg acgctcggac ttctatggac cgtctcgcgg ttcctgagct
    26461 tgggcggctc ggtggacctg ccgtgggtgg cggacgcgga gcagaccaag accatccggg
    26521 atacggctac gacttacaat gcggcccgca cgcgcgtcct ggacaggagt tccaccgaga
    26581 gccttgtgtc caaggacgtg gagatcacgt tccccgccta cttcgccctg ggcgccgtgt
    26641 ggcgatggac ggaccgcctg tatacgtctc tggacgtcag tcagacgctc tggtccgatt
    26701 tcgccttcca ggccgacggc gaggaaaaga tcaacccgct ggacggcagc ccccgtggcg
    26761 agaacccgct cgacgacacc tgggcggtgc ggtccggcat tgagtacctg ctggtcttcc
    26821 cgcgcgtgga aatccctctc cgcgcgggcg tcgcatggga ggagcgtccg gccatcggcg
    26881 aaccggacga atactggagc gccagcgtgg gaacgggcat tgccctgggt cgggaaccgg
    26941 cgcgctggat cctggatgcg gcgtacgtct tcacgcaggg cgatgatgtg ctgggcagtc
    27001 tcgttacgga ccaggatctt cgcacagacg tcaccgagca tcagtttttc gtctcgtgca
    27061 tcaagcattt ctgacgcgcc cgccagcgga gaaaaggggg cacagcgccg caccgaatca
    27121 aggcgttcag ggttctgcac ctcttgggcg ttgtatcaca gtttataaac tgtgatacac
    27181 gggatcggcg ttgatatttc ttagtatttc ctgatatttc tccatccaaa atatcacagt
    27241 ttataaactg tgatacatgg gttttcaccg tgccgcggcc cgtcaggcag ttgagcgaat
    27301 caagcagcag gatccctgtc tcggcggcga acgggaacgc attgaagcac gaacccttgg
    27361 caagaggtcg ctggccgact cgctcggtca aaacggcaat atgtctgcct ccggcctagg
    27421 gacggaattc ctcgatacgg tagaggcgcc gcccttcgac gtccggagcc gtccactcca
    27481 tctcgctgcc gtcccagttc gtccaagggc cggcgatacg cgcccatgat tcgtcgagca
    27541 ggttgctgca tgacgccacc tggtacgcat gagctaccgc tcccagccac cggaaactcc
    27601 agccaccggg cccccagacc gccccgtcct ccggtatcgc aaacacgctg gcccggtcat
    27661 tgggatccgt atccgtgagc agttccgtcc agttgttggc cccgtcgccg tcggtatcct
    27721 cccccgccgc gatgttcgtc ggccccccaa agtagaggtt gatccagcgg gctgggaggc
    27781 tgttcccgtt cgtgggaatg ggcgcgcagc acaggtcccc cagattggtc accccccccg
    27841 ccggcaaggt gggcagcgag aacgccaggt tcgagtaccc gtccgcgaaa accgtcagca
    27901 cacccgcccg cgtgggcaga tacacgttgc tcgggaaggc cccctccaga gacgttgccc
    27961 agtattgccg gtaatccgca tacgccggat acccatcata ctggctgcag gggaggcccg
    28021 aggccgaccc gggcacgaac cggatccgcg cgtttctcaa ccgccggccc gtccactcgt
    28081 cgcgcgccac gccctgcgcc cgggccagcg gctcgagaag gaagggggcc gaaacaaagc
    28141 cgccagcctg gaccacgacg cgacgcggat tcccgtaggc ctggttgttg atgttctcca
    28201 cctgcccggc ctgggtggcc ttctgcgccg gaagacagcc gggcgccgtc acctggatcg
    28261 tgtacgtccc tgccgcaatg ggggtgaact ggatcatgtt gcgacccccg aaggcatacg
    28321 gcgtcccgtt gaggtacgcg accgccccgg ccggcgagcg actcgagttg tacaggtccg
    28381 ccccgaggat gcagaggaat gcctgcgcgg gcgcgtccgg cacgtacacc cgcagttcgt
    28441 actccgtgcc cggccccgcc tggcccgctc cgtatgcgcg aacgcgcgcg acgtatcggc
    28501 cggccggctc gtgatccagc aggatgtact cgcccgcccc ggcgcccgcc ccctgcgagt
    28561 cccgggacac cacgttcgtc aggacgccgt cctccacccg gtagaggtcc agcatcgtgt
    28621 ccacgttcgt gcccagctgc cgcgttcgga cctcgtagac gaagtccgtg atgacgaaga
    28681 actcgaccca gtcctcgtcg ccggccacat gcaggttgtg cacctgcgtg cccgcaacct
    28741 cgaagggctg gacctcgtcg aaggaatcgt ccggctcgta gcggtcggcg ctcaacacct
    28801 gggcctggcg ggggctgccg atcacgccgt cgtggtccat cgcgaagaag gtgcagatgt
    28861 acacgcccgg ctcggtgaaa ttcgtgtagc tcgtctcgta ccgggcggtg tcgttactcc
    28921 aggacaggtc cacgctgacc gaagcgggat cctccgcgtc cgacggcggc gtgaccacgc
    28981 accaggcgcg ctcgacgccc gccgccgcga cgatctccga cgcccacagc gtcaacgcgt
    29041 tcgtcccttc cagcagggtg tcgagcgtga cggccccgat caccggcgct tccgcgccgg
    29101 tcacaaaggc cgacccgatg taggtcacgc ccgccagcgc gccctccgcc ttttcgttcg
    29161 gttcctggtc cccgtcgtca tccagttgcg ggtcctgcgc cccgcgcgcc aggttgaagg
    29221 tcgtgtcgcg cgccgcctgg aaggcttcca gcacgtccgc gccgctgaaa atgtggctca
    29281 agaaaatgcg gctgaaactc aaccatcctt gctgctcgca gagggctggc tcgtccggcc
    29341 cggacccggc gatgaggatg cggtcgcggt ccgggggaat ggtcatgttg gtgatgaacc
    29401 cgcccgcgcc cgcgaaatcc atcaccgcga tgacgagccg gttgctgtcc tgctggtagg
    29461 cgtccagcca ggcgtccaac tgcgcggcgg acagatcctc tccttcggca agatgcagct
    29521 gtccgccggt cgcctcgccg gcgaggtaga tcgccagctt cctcgaggca ccggcccagt
    29581 tcgtgatcgc ctgcccgagc gccgtgagcg agacggcctc gtcgacgccc ggctggccgg
    29641 tctccatgct caagtaataa acggagtccg tggtgaactg ccgcgtctgc agggtctccc
    29701 aggcgttgtt cgccatgccg ttgatgttgg tccagacggc gaggttggtc gtcccgccgg
    29761 ccgccaggat cgcccgctcg tccgcgcccg cctgcgtcac gtacccgtac ttcggcagcg
    29821 agacgccgcc gccctgggca tgcgcgtagt agatgatctt gtaggtgtcg gcctgcccga
    29881 agccctggta gaccgcctcg tatcgttcgc cggtgaacac cagatcgatt tccggaaggg
    29941 aggcgatggg cacgtcggga tcgggattgt aacccggcgg cacgatcacg caccagacgc
    30001 ggtcgatcgg ctgcgcggcg gtcacgttgg ccgcccacag cgtcgccgcg gaatccccgt
    30061 ccaggtactg gttgcccgca atgctcccga tctgcgggac atcctgctcg ccgccgatcc
    30121 gcgcgccgac cggttcgtcg aggccggccc cggcgtcatc caccccggcc acgaagagcc
    30181 cgtccttgtc cgcgtccagc cacgcgtcct ggtaatcgct catggctccg cgggccaact
    30241 ggaacgcctc gcccaggccc aacccgagcc tcaacccgtt gaagaaggct tcggagaaac
    30301 tcaccaggcc gccggccacg aagaagacgg cctggaccgg tccggcgccc gcgatcacga
    30361 cccggctcgc ggcgccggta taggccagcg gcccgataaa actcccggcc tggcagaaat
    30421 ccagcacgac cgtcacgtcc atcggcgcgg cgttctgcaa ctcgtccagc caggcggcca
    30481 ggccggtcgc cgagagggtt tcgccgggat tcagccggaa atagccgccg tgctcgttcg
    30541 tggcgccgtg gtccacgagg tagacgaaga ggttcgaggc gccggacgca aaagtgcgga
    30601 aggcccactc ggcgttggcc cacgtggagg cccgggacac atcggcatac ccatcgccgt
    30661 ccacatcttt ccccgccagg gggctcaagt actggatgct gttcttgctg aagccgcgcg
    30721 cgcgcagcgt gttgaaggcg ttgtcggcca ggtagtcggt ggcctcccag gccggatcgt
    30781 ccgtcagcgc ggccgtcttg ctgccggcca tgacaatggc gcgggcctgc cccgcggaca
    30841 ggctgttgct gacgacctgc gcgaccgcgc ggccgtaccg cccgtcgacc aggcgcgcct
    30901 cgatgatgtc gaggccctgg gtcggcccgg cctggtagac gcccgtgccg gcgttcaggc
    30961 tgccgccgct gcggttggcg acgaactgcc aggccacggg gggatcattg gtcacattga
    31021 aatcgcacgt cgagcccggc tccatcgtgc gttgcgcggg gatcacctgc agcttgtccg
    31081 cctggttccg gtagaacagc aggccgccct cggaggtcgc cagcagcagg tccaagtcgc
    31141 cgtccgcgtc caggtccatg gcagcgccgg actcgacgaa atcgttcaag ccatacgtat
    31201 cgggcggctc atacaggcgc atcgcgtggg tccaccgcgg ctgctgcgcg tcgccgatgt
    31261 tcggataata cacccagtcc cggcccgtca tgccgatcag gtcgttgtcg ccgtcctcgt
    31321 ccatatccat gacgagaggc gcttcgatcg agtccccgaa gaaaccggcc ccggcggcca
    31381 ccggcggcgc ccaggccgcg acggaagccg tcccggtgtt cgcaacgtag aagaatggac
    31441 cgttggtcga accgctcttc aggtacatgt ccagatcccc gtcgccgtcc atgtcgccga
    31501 aggccatctg gccagcattg tagtagctgt tcgtttccgc atggagcacc aggtagccgt
    31561 actcgggggc ggcccacgcc gcgcgcgacg ccgtgccggt gttcaggacc cgcgcgatga
    31621 accagttggt cccatagggg tccccgctca tcttgatgta gaggtccgga tccgagtcgg
    31681 cgtccaggtc ggcaaaggac accgcctgca ggaccccgcc cgtccacgac agataattgg
    31741 tgtcggccag gcgccacatg ggcgtctgcg gggtgccttc gttctcgtac agccagagca
    31801 ggcgctcgac gcctttgagc acgacgaaat cgccgtcccc gtcgccgtcg agatcgcaac
    31861 cggccatgct gtcgccgggc aggtccatca agaagatccg ctcgccccgg acctgccagg
    31921 cggcctgcgt ggcctgcccg atgttcacga gatggtccat ccggccgccc gcctgcccga
    31981 cgacggcgtc cagatcgccg tcctcatcca ggtccgccag ttcgggggca aaggacgcaa
    32041 agaagtccgt ggcggcgatc ccgagttgct gcgtgacggc gggcgcccac cgggcctggg
    32101 ccgccgttcc cgtgttggcc cacaacagcc agaatgcatt ggtgcttccc gggggatagg
    32161 gctcgtagcc ggccacgaga tcgaggtctc cgtcgccgtc cacgtccgcc ggcctcggcg
    32221 aggcataccg gtaattggcg atggggcatg ggaaattggt gcggggctgg ccccaggccg
    32281 ccgacgcggc ggttccgata ttggtgtaga ccaggatatc gccatccgaa ttgccaatga
    32341 agagatcctg atccccgtcg ccgtccacgt ccgccagggc cggggcgctg aacgaccccg
    32401 ccgtgatccc cccatacgcg aagaccggcg cggcccacac ggccgtggtg ggcgtgccct
    32461 ggttttgaaa atagtacagc atcccgaaca actcccccga gatcaggtcg aggtcgccgt
    32521 ccgcgtccat gtccgccagg gccggcgcgt aatcagtatt ccacgccagg cggcggacca
    32581 gcgacggggg cgcccaggcc ggcgaccgcg gggtgccgat gttcttgtaa tacatcagct
    32641 tgtcccggct gccgacgaaa gcgtccgcat cgccgtccgc atccatgtcg ccgaacgccg
    32701 gcttggtgct ccgccccccc ccggaatacg cgccggccag gtaccgccgg tccacgtagc
    32761 cgtccaggac cggttcccac gtcgagtcgc cgaggatgtg gatcacgttg gtgatcacca
    32821 gcccgttggt cgaattcagc ggacggtccg cgctgatcct cacgaggttg gtcagcaggc
    32881 ccggccgcgt ggcccggatc gcatacgaga aagaggcccc ccacgaaggc gtcagggtgc
    32941 acgaactgca cagctggttg ccgttggcgc ggaagtagcc gtccaggatc tccacgggcg
    33001 ccgagaactc gctgacgatc aggacattgg agacgtattc ggcagagagg ttctccaggt
    33061 gcaccttatt cgtgaacggc cgggaaagac aactggcctc ggacagcgtg acctcgacgc
    33121 cgatgcccac ggccgggccc gacgggggca ggaagatggt cgcgaaggcg gcggcgttgt
    33181 cctccgcgtc cgggtcgcct cccgcggagg tcacgctcga cgtccacgcg cagtatcccg
    33241 tcaccgccgg cgagcagacc atccagtaca gcgcggtctg gccggcggcc atctccgtca
    33301 ggcggaacac aaccgccgga tcggccggac ggaactcgta gcccaacgag gccgtcacca
    33361 cgcccacgcc cggcggcagc cggttggtga tcgagacctc gacggcctcc cgcggccccg
    33421 cgttccccac cgtgaccagg agcggatagt ggttcgaaac cgcacggccg ggtccccaga
    33481 aaacgggctc cagggacacg gacaggtccg ccggcggcgc cgcggccatc gtctcgatcg
    33541 cgatctcgtc gaaggcgaac ccgtcttcct ccgccggcga gtcgtcgtac tgctggaact
    33601 tgatcttgaa cccgtcgcac aacggccagc ttccgtcggg gaccaacggg acctcgaacg
    33661 gcttgtaact cgaagacacg aaattggacg aggtcaggcc ctgtaccttg tgccacgtcg
    33721 cgccgccgtc atcgctgacc gccacgccgt ccgcgttgca actcccgctg aacgaatccg
    33781 gcatctcgtg gtcttcgtcg ttgagcgact tgtgccggaa ggacaggacc acgttgctcc
    33841 gcccggccag gtccgtggtg agcaccagtt cgttcaggga gggcgccagg tcgctctgga
    33901 catcgtccat caccgcgtgc cacgcgccgc cggcggggga ccactctccc gtcacgcgga
    33961 ttcgcccctg gccggcggag ttcgtcgccc agcagggcgc gagcgcgccg gattcgaaat
    34021 catcctgaaa gaggatttca gccggcgagg tcggcgggaa atggaacaag gcgagggcga
    34081 tgcccagcag caaggcgctg ccggaggcgg gccgacacac cctccctttc cgccatctga
    34141 ccgtggcgtt cattcctgtc atccgcgctt cactcctgcg cctgaacaca atctatgcag
    34201 ccggaggccg gccgtaaatg ccattcggcc ttccgccccc actttctacg tcgtccgggc
    34261 cgcccttttc ttgtcgagga accatgtaat ccttgacacg attttcggcc ctcaaaccac
    34321 cacgagttct ttagtcgccc ggcgcgctgt tttcgtcccg gccctgctcg ttccggggaa
    34381 gaacacccgc gggtccgtcg actcttgatg gtaaccaacc gaaaccggac aggagcaacg
    34441 ccatgccatc gaccccgcgg aataaactgt cggcctgggc ggccgcgctg tgcgcagcgc
    34501 tcgcggttct gccctccatc gcgatcgcag atgaccttgt cgtgctgtac ccgcgcatcg
    34561 cgcacgaggt gcaggccccg ggcgtgccca tcgtggatga cggggcgaac cggtttgaaa
    34621 tctacaacga cggcctcggg ttcatgagcg gaggctccct ggatgccgcg ctcgggcagg
    34681 tgctgacctg gaggaacttc caggcgcccg acgtggtgtt cgatccgatc caggcccgcg
    34741 ccctgctgct cgggggcagc cggttcgggc gcgcctcgct ctacggcgtc catttcccgg
    34801 acgccgcggt gaactatgcc acgccgatcc ccccgccctt cgacggcgtg gactacacca
    34861 actcctcccg cttctccagc ctcctggtcc cggcgcgcga aagctgcgac gccgtcacgg
    34921 agggcttcct ctggacctgc tccagcgaga accagttgac cctggtccgc tacggctggg
    34981 acggccgcgc cccggccggc cgcgggacgg tggtggatct cggcgcgcgg ggatatcgtt
    35041 actcgtggga tcgcttgtcg ctgcccttgg attacagcca ggacacccgg gtcctggact
    35101 atgatccggc cccgggcgaa ctctgggttc tcgcgctgac cttccacgaa gaaccgggca
    35161 cgatgggcgc gttctcgaac agcctgctgt gcctggacgg cgcgacgggc gaaatccggg
    35221 cggaggtcgc gggcttccgg cacgtggaca cctgggaaat cgatccgcgc aatcgcacgc
    35281 tctgggtcgc ggaccggtcc tccatcatgg gacaggactt catctaccgg atcgaccttg
    35341 ccggcctgaa ggccatgcgg atgaacggcc tgacccgttt cgcccccgac aacccggcct
    35401 ccacgcggct gtcctcgagc gcgttcggac tggaatacat cctcgacatg agcgtcagcc
    35461 cgctggatgg cgcggtctgg atgctctcgc ggcgcgcggg gacaggctac cggctcgcca
    35521 aactcgacgc cgccgggacg ctcgcgtgct ccaggaactt ctccgactcc ggccgcgccc
    35581 cgctgcacga tcccgactac ctgctcgcgg acgacgccgg cggctgctac gtggcgctcg
    35641 gatacgccgg gaatggatcg gtcctgccct ccggcgagcg ggactacgtc cggaccagcc
    35701 tcgcccgctt cgaccgcaac gccgcgctgc ggggcgcggg ctggcagacc cgcacggtga
    35761 gcaaccgccg ctaccgcgcc gaccggcgcc tgatcctgct cgagaacgcg ccctgctccg
    35821 agcagcagat ctgcctgtcg gcggccccct actgcccgcg gcagcggcag acggtctcga
    35881 gccgcgtcct gccttcggac tgtcagggcg tctccaagat tgcgtgggag gtcgagaaac
    35941 tggacatcgg cctcggcacg gtgctgggcc tggacgacgg cttcgacacc gtgtggtccg
    36001 acgagaccgc gttctcgccc tgccgcgcga gcgcgcacgg ccgcagcgag gacctcgggc
    36061 ccctgggcag cggaacctac cgcttcacgg tgcgcgtgac cggctgcaac ggcacgacgg
    36121 gcaagaacga gttgtggttc caggttcccg cggaatgccc gccgacgacc ccgccgggca
    36181 gcgacggcgt ggcggtcgag ccgggcgatc ccggcgaccc gaaccgggcg acgttgtgga
    36241 tcgccctgga tcccgtcccg gccgccgtcg cctcctcggg cgggcgcccg gcctacatct
    36301 tcgaccagta cctgcgctac cggatccagg ccgcagcgcc gggcgacagc attgaacgcc
    36361 tggcctggcg ctggattcat ccggcggacg cacggcgcgg cgcggcgcag gaagtatggg
    36421 atgcgcgaac aaaagacggc tggggggatt tggcttcctt ccgcctgacc gattgcatcg
    36481 gcaccgatcg ctggggcctg ccgctcgcgc acgacttcgg cgagtaccag ctcgaggtca
    36541 ccctccggct ggcctcgggg gtcgacctga cggcccggag cagcaagttc tacatcctgg
    36601 gccacaagga cgcgaaggtc gggatcgccc cggcgccgct ctattgcgcg gattcgtcct
    36661 atcgggtccc gttgtacgcc tggagcgtcc ccgagtatgg attcgacacc gtggacgagt
    36721 tcgccgtcac gtgccaccac ggcctgggcc agcaagacct ccggtccatc ccgtacgcgc
    36781 ccgccatgga ctggtggcct gaggagcccg ggcaggcgga gggctggtgg cgcatcccgc
    36841 agttcctcgt cgagtcggac caaccctcga tcaccctcaa ggccacggcg acaaccctcc
    36901 ggtccgacgg cacaccggtc ccgctctcgg ccgagacgat cgtgctcctg ccctgcccga
    36961 tcaacgggct gctcggcagc acgcatgccg ccaccgccaa gtgccggaag ttcaaggcca
    37021 aaatgctgat gggcagcgcg gagggccgga agatcatcag cgcgttctac cggctcgcgc
    37081 ccgagatgag cgcgctgctg gccaaggacc gcgtgttgag cagccagtgc gcggcgttcc
    37141 tgaaggccgt ccagccggat ctcgcggcgc tgatgcagaa gggcaacccg aagctctcgc
    37201 cgctggcgcc ggagaacctc aagctcgtca cgcccaagaa catccaggcc gcaaaggaca
    37261 tcatgacgcg cgtcaagccg gccgcatcgg ccgacctgcg gagcgtcatc gacgaactgt
    37321 tccgcctgct cgcggccgcc ggcaagtaag ggagagatca tgcactcgat gacctgtcca
    37381 agggggacac tggccttgct cctggcgctc ttccggttcg cgcccgccgc gcgggacctg
    37441 ccggtcaaga ccaggcctta tgcctcctcc accctgcgca cggcgcccaa cgacctgatc
    37501 aacaagctcg acgccgccaa atgaaccaag gagatcccgt catgcagaga acgcattcga
    37561 ccccgacggc ctgggcaagc cggttcctcg tcctggtctg gttgttggcc ggcggcgccg
    37621 ccgcggagtg cggcttcccg tcggacacgg ccgggtggct ggtcttcccg cgccaccgtg
    37681 atgccgcatc gccgggaggc ctctgggggg cgaaccagct gtacacgctg gatcacagcc
    37741 tggccatccg cagcacgcgc acatggtcca atgcgtcggg ccagacgccc ttctgggaca
    37801 gctactgggc cggcggccct tctttcgacc cggaaggctg ccgggtctgg atggcggacg
    37861 gcgaccaacg aagcttctac accgtggata tcgtcacggg aagccggcac tactacaccg
    37921 tggaggcggc ggccgatcgg gacatcctgt ttgacaagga ctgcctctgg ctggcctacg
    37981 actggatccg ggagcgcctc tgggtctccc ataatccatg gatcatttcc gaagaaaacc
    38041 accggatcat gaaattccgc atcgagggga cgactctggt caaggaaagc gatctggatc
    38101 tgcaagcgct cggatacgaa ctgccggccg gtcccgacga aaacggcatc gtccgcgatt
    38161 cgcggagcat ccggctggac ctggtcactg gcgacctctg ggtcctggcc gacacaagcg
    38221 aggacggcta ttccactccc aacatcctgc tctccatgga tggacaagat ggccgggtga
    38281 agtgcgtgtt gaccggtttc gatccgggca ttgatctctg ggatctcaac gaagacgagg
    38341 gttcgctctg ggcttcggtg ggcaatttct acggcgcagg cgacggggcc ggcacgctgc
    38401 tgaaattcga cctgcgcgcc ctgccttcgg gcgtgtatga cggcttttcc gccgccgtcc
    38461 tcgccgcgcc cctctcctcg ctttacctgg cctacccggt ggacatggcg gtatcccgtg
    38521 tggacggcgc gctctggatc tgggacgcac gggatggcct cgagaccggc agcaacgccc
    38581 aacagcattg gaggctggcc cggctttccg cgaccggcgc gctgacgggg agttccgatc
    38641 tcacgctgga tgtggaggct gatacgggca ttacctgctc cgatttcatc gagaccacgg
    38701 ccaacggagg ctgtatcgct gccctggcct acaccctgcc cggcgggaac gaatccaagg
    38761 tgcgcaccgc cctgtacgac gccggcctga accggacggc gggctggcgg gaggttccca
    38821 ttgggaccaa ctggggcttc tacgggcgtg gggcagcgct cccgcgcatc ctgacgccgc
    38881 cctgcctgcc gcccctggcc cacgcgtcca cggaccggcc ccgctatcgc ctcggggaaa
    38941 cggtccggct cacctgtggg gccctgctgc gctgcgaagg cgtccggcaa ctcgcatgga
    39001 gcgtccgaag ttccgcgggg acggaggtcg gggcgggagc atacccggcg ccaggcggct
    39061 gctcgtatgc ggacgcaacg cagacggtcc cgctcgccgg actgccgctg ggcgtctaca
    39121 ccgccaccgt cgtctgcacg agctgcggga gcctgcccac cacggcgaat tgcgtcttcg
    39181 aaatcctcgc ggcggccccg ccccggccca cggtgagcat cctccgcgag ccctacatca
    39241 cggcctacgg gaatcgacag gatgtgcgct tccggctgcg ggcggacgcg cagggaagcc
    39301 tgatctccga gttgcgttgg tgggcagagg acaccaacgg cgtgcgggcg ggcgcggaac
    39361 agaatgcccc cgcgtgggga cgctccagtt acaacgaacg gcacatcctg tccccattgc
    39421 ccccggggga ttactacctc aaggcccgcc tggcttccga ttggggcgac gtcgtggaag
    39481 ccgccagcgg acgatttcgc gtatggccct ttagcaagga ggcccgcgcc ggctttgatc
    39541 ccgaccgcta cgtcatcctc cgctacagcg acacctgcgt ccccgtcgcc ggctgggccg
    39601 ccgccgacaa tgggctttcc ggttatgggg tgcgctggga ggggtatggc ctcgccggcg
    39661 acgccttgcc ctggcgctcc ttctccgccg gctgggagcc gacggccgac tactgggagc
    39721 cgggctgctg gcccatcccc gatgccatct ggccgaatgc gcagacccag gacgtcgcgg
    39781 tcttctacct cgtcggccag gccggcgccg cggggtcgcc cggcatcgtt tccaaccgcc
    39841 ttgccgtcat cctgatgtgc cccctcgaag aaatcctggg cctcgagagc gccgcgacga
    39901 agagcgcgcg cgagttccgc gacctgttcc tgaagaaaag caaggacggc aaggccctcg
    39961 cggaccttta ttacaaacac gcccgcgaat tgagcggcct ggtcgcgaag cagcccgcgc
    40021 tggcgaagca ggccggcgcg ctggtcaccg ccgcgctgcc ggacatcgcc gcgcttttga
    40081 agtcgggcaa accgacgcgg ggcccttggg cgaaggagaa cgtcaagctg gtgaacgcca
    40141 agacgatcgc cgccgcgcgg aacctcctgg ccaagaccaa gccctgcgcc tcggccggcc
    40201 tgcgcgcggc cctcaacgac ctgatcaaca agctcgacgc cgccgtcgcc ctgcgttgag
    40261 ccggacggcc ggcggcgggc gaaacgccgt caataccgga gcgcgtcggt ccggtactcg
    40321 acgcgcaccc cgtggaattg ctggttcacg ccgtcgaacg tgcccacgct taactcgact
    40381 tcgacccagt aacgggtgtt ctcgttgtcg acgatatcat agggagacat catggcggtg
    40441 tccgtctcga tctccgcatc actgcccggc gaactcgccg ccacctgctg caaccaggcg
    40501 atgtactgga tcccggacac ctcatattca tgccgggcca gggccacctc gcacgtcatg
    40561 gcgtaggagc cgagattccg gcagtagctg gacacggccg cgatctccgc gtcctgcggg
    40621 aggtggacgc cggcggcaaa cgaagccgat ttcggcgtgc cggtcaggta caggtatccc
    40681 gagctatacc ggtagttgtg gtcggtatgc tgcggaacga actcctgggg cgggatccac
    40741 aggtagttgg tccgggtata cgcgtaacga tactcgccgg tgatcgtgac gctcccctgg
    40801 accgccaggc tgcccgcggc gtccacgacc aggccggtcc gcgtgccgtt gacgaagccg
    40861 aggggcgagg cgtagtggat ctgcgacgcg aggtagagct tctgccaggg ataggaagcg
    40921 cttcccagcg cgaacaggtt gctgccgtcg ggcaggatgt ggccgttgac ggtcagcgcc
    40981 gacatcaggt tggtggtgcc cacgccgacc cggttgttgc gccgcgacac ggccaggcgc
    41041 gggccattag taagcacgga gtctttctcc aggatctgca gctgttcgct gtccacctcg
    41101 tactgcaggt acatgccgta gatggcgccg cgatcctccg cgagcaggat ctgggcgccg
    41161 tcctcattgg cgttcgtcgc cgcgggcgac acgaccaggg tggcgatctg gccggtgcgg
    41221 ccgctggcga agtggatgtc ccctttcaga ttagtggtat tcagcccgaa cccacgcccg
    41281 gcgcgcacga ggaactggtt ggaggcggcc gatccgaaaa cgccgtcctg ctggtcggcc
    41341 cacacgaagg cgccgggatg cgaggcccgg gcgccgccgc cgccggcgaa gctgtagtcg
    41401 ccggaggcct ggttgtcccg tccgcccggc acgacggcga aggcgccgga caggttgccc
    41461 tgcccgccgc ccacggcccc gtacgcgccc agcgcgaggt tggagccgcc gccagcgacc
    41521 acggaacccg agctgcgggc cgtattattg gagccgccgc cggtcacact gttcgggccc
    41581 tgcgcaagat tctgctgtcc gccgccgatc gcggcgaagt gggcgtccgc gtgattccgc
    41641 aggccgcccg ccacgacgcc ggcggtcccg ttggacacgg tgttgcccag tccgcccccg
    41701 atgaatccgt aggcgtcccc caggaactgg ggcaggctgt cgctgccgcc gcccgcgatg
    41761 gcgccccctg tgccggtcac ggagttcgag ccatgtccga ggataatcag cggactgctg
    41821 gcatccggaa aaaaccgcgc gacgcgggcg tcgtgaaccc gcagttcgaa gggccggtca
    41881 tccagcgtgc ccgcgaaatc cgcgcccagg ggcgggggcc gcgttccggc gttaccgccc
    41941 accgtccaga aggtattgga aaaagacccc acgtccaggt ccggcggcac gacttcaccg
    42001 ttgccgatct tgtcgctggt gaccgccccg aaggccaggt tccggttgct gatgaccccg
    42061 tccgtgaggt ggacgttcac gatcgagttc gtcgcaatct tggcctgggt cacgacagcc
    42121 ggccccagca tcgcggacga tatggcctgg ttcgtcaccg cggcggcgat cagggcatac
    42181 gccacggcgc cgacgcgttc ccggggcatc atcggggtgc cgttgacggt gagagcaagc
    42241 cacacctcgt cacgggtcag cgccaacggc agggagccgg acaacgggtt atcgccgatg
    42301 aaggtcgcgt atagcccgtc cacgacgggc acgtccacat cctgctccgt gtacagggcc
    42361 gtcccgccga cctcccccgg gtagaacgcg aagagcagcg tggcgggccc gttatacaga
    42421 ttggtcccgg agatcaaccg gccctggtaa tggatctggc tggagacctg cgccccggcc
    42481 tggagaccga tcacggccag gaataccaga acgccccgaa gcaccgaccg tcttttcata
    42541 ccccctcccg cgtgctggaa ataccgcgtt cctcgtggag gatatacata ggggcgcggg
    42601 ctgaaacggt caagagcaca acccctgccg cgccggttcg gagaggaaat cggtccgcgc
    42661 attgctagtg tgaggagggg aaggtggagc gggagacggg agtcgaaccc gcgacgttca
    42721 gcttgggaag ctatccggaa tatgccgttt ttcttaatgt atatgatgcc gttgacaatt
    42781 cgttgacaac atgccctctt cggcgcaccc tctcctccat aagcaaggat gcgccatgaa
    42841 aagacggatg cgacgaagct gccccgtgga gacttgcgcc ggcattacga tccgcgagat
    42901 caagcccggc aaatggcaga ccgacctttt ccgcgacgga aaacgggatc gccgggtcca
    42961 tgaaaacctg gagatggcca agcgatacgc gcagctcttg gccacgaaga tcaggaacga
    43021 aggatccagc gctttggacc ttacacagga gcagagacgg gacgccctga aagccttggc
    43081 gattcttcag gacaagacga ctctagcctc cgccgcgcag ttctggtcct tgcataacca
    43141 taacgaggac ggggtcacca tcgagcaact cggggaacgg tggctggccg atctccgccg
    43201 gcggggatgc cgcgagacca cgctgcgcga gcggaagcac aagatcaccc ggctgaccgc
    43261 cgactttggg aataggcccg cggcatcact cacccggcag gacctcctgg attggctcga
    43321 ccgtcagggg ctgaccggtg ttacggccga tggataccgc cgagcctaca agacgatgtt
    43381 caacttcgcc atggacgaga agatcgtcga aatgaaccct gtggccggca tcaaggcttt
    43441 caagacggac gagcggctgc cagtcccgtt cactgtggcc agcgtccaaa cgataatgga
    43501 cgcggcggcg aagcatgccc ccatcatggt ccccaccctc gctgtccagt tctttgccgg
    43561 cctgcggccg ggcgaagcca aagggctgaa atgggaggat gtcaactgga cggagcgtac
    43621 catccgggtt ctgcccgaaa cctccaaaat gcggcggtcc cggcttgtgg agatgaacga
    43681 cacactccga gcctggctgc agccctatcg caagaaggac ggtcccatcg ggatcacctc
    43741 gcagaaccag ttcgatttct acatgagccg aaaatactgc ggggaaaaga agatgggctt
    43801 gctgaaagcc gcaggagtcg aatggataca ggacggtccg agaaagacct tcgcgtccat
    43861 gcactacgcc actcaccagg atgccgccaa attagccgcg atcctggggc atacgggcga
    43921 cgcgggggtc ctgttcaggc actaccgcgg ccttgttaaa ccctcggagg cggctaacta
    43981 ctggcgtctc cggccacgaa ccaagaccac cattattacg ctggaggcca cggcatgaac
    44041 aaagcgagat ccgcgcacac ccgcagattt catgatcgcg ccgttgagct gatcgcgtac
    44101 tacgaggact ccgcagggga ttggtcggag atacagcagc tcttctttgc tttagagaca
    44161 tcctttgatc ccgctttcta catcacgcag cgccggcctg gatcaagtcc agcccgaata
    44221 caactgcagc gcgacaaagc tatcgcggct aaacaagcga agctggcgca ggctctttgg
    44281 gccttcgatc tcggcgtggc aaaacagtac gccgacattc tcaatcaatg cgagaaaccg
    44341 ccggggataa gcggtgtcga ggcccgctcg ccctttttac acgaaatcgg aaagctgaaa
    44401 tcgccaagct tggctaggca ggaaacggag cgaaggaagc gaagccgcga cgcgaaaaag
    44461 cgcgggaaac tcggcgggcg ccccctgaag gacggcaacg aagaaaagtt aagcaaggca
    44521 ttgcgcgaag ccagacggat gaaacgatcg ggcttgtcgc ttaggcgagc ctgcgagttg
    44581 gcggctaagg agtctggttt gactgtcggc tgggaagccc tgaagaagca tttctatcga
    44641 gggaataact aacctccaag gttattccct ggtttatgat ccaggccgga cccaacaagt
    44701 ccggcccttt tttgcatttt tcagggacaa acctaccgca tgtcgttttc ccggactgta
    44761 acataagcgc gagaaaatgc gcgtgacacg taatgcggga gaacgaatga aaaataacac
    44821 agacttggca ggttacttgc ggaggagaca ggccgcagcc tatctgggga tatcccagcg
    44881 atgcttgtcc gactggcaga aacggcgcct tgtcccttac acgaaggttt cgcatcgggt
    44941 ttgcctcttc cggcgagacg atttggacaa agcgcttggg aagttgaaga ttgaggccat
    45001 cgggaactga cgccgcccaa gtccttgcat cacgagaggc attctcgacc atacaatgtc
    45061 cagggggcaa tcaaggccat gaagaaccaa gtttgattca ggcgagcgag gttcagaagt
    45121 gaatgacgtt ctgatttcaa cggcagaggc gtccagccga cggctggaag accttcgcga
    45181 acagttggga atggacagca gccccgaaga tcgcatcgtt gttctcgggc acctgctgac
    45241 ttggtacgcg aaatgggccg gggatccgga aacggattcc ggtctcttct ggaacgtgac
    45301 agaaaagcgg cttggggtgt gggctcagtg gacgcgctcc aacccgctca tgtttgggcg
    45361 cgcgcttgtt aaggcggggt atgtgcagcc ggtgaatgac gtgttcccgg aacaactgct
    45421 acgcaagcgc gatggatacg tggttcccgg ggcgctggat cggtcctgga gcatccaccg
    45481 attgagggcc aacaagcttg acctagtcct cttcctgctt gatccggtgc gacgtgcccg
    45541 tcaagccgcc ttcctgggac tgaagccaac tgatgcccct accggctggc tcgacttctc
    45601 aaggctgtct ggcgacgtgc cggggccggg agaaggcgca tatccatcct ccggagggcc
    45661 tccggaggat ctccagagta attccgtagg ggcttcggag tcgcccccca ggggctcccc
    45721 ccacgtagac gtaaacgtag aaaaaaagaa acgaatgttt ccacgtaaat acgtagggaa
    45781 caaaacgacc acgggagctc cgaagactcg cgatatgttg gagtatattc aagagaacaa
    45841 gtacgagaat ccgctggagt gcctgcgcac gattgacgga acggacgggg ctgtgccgat
    45901 ctggatcaag ggcgtcgagc attgcctttc ggatgtccag accatgctgg ccaacatgac
    45961 ggaaacggac gaggcatggg caaagatcgg caacccagcc gcggttgcca tgtctcggat
    46021 caaggccgtc cttcgggcca ggggaatacg aacggggaac ggacagcaag cataacggac
    46081 acggaggggg gagcccctgg ggggctgctt gagaacttcg cctcaaatcg attatcctga
    46141 ttgtaggcag gccagaagtt gaggccacga atgccctcgc gcgtccgtcc gcgcggacgc
    46201 gcccgtccgt ccgcgcaggc acggacgtgc gcgcgcgcga ggacaggctg cactggattg
    46261 tctgatcgag aacgcggtat tgtgcagttc aatcatcgtc cggccagtct gccgcgttgc
    46321 gtccaccttt ctgtccggcc agatcctgcg aagtgatcat gtcgggatgc tgcgctgcga
    46381 ctctgtgagc aagtcgaggc acgattgctg gaatgccgga cgagaaagcg tgaccgatcc
    46441 gccccgtttc ttttggcttt ccccggccct gtctggtagc gtgcgatcga tcacgattca
    46501 tgccgatgcc aaggcggcgg gtggcctgca caggaaatgc gagaagacca agactgaccc
    46561 gtaggtcgaa tcgggcatgg cagggccgaa aatgaggggg gaacgggttt ttcgaacatg
    46621 gggatgacgc tccaggaact gaagtctcac ctttggaact gcgcagaaat tctgcgcgga
    46681 agtgccgtgg accggacgga ttggaaggcg tatatcctcc ccctgctctt cttcaagcgc
    46741 atctgtgacg tgtgggacga ggagacgtcc gcggcggcga aggtgtatgg ggacgtggac
    46801 cccgtggatt tccccgaggt gcatcggttc agcgtcccgg ccggatgcca ctggagcgat
    46861 gtacgggaga ccccggccaa catcggcgcc gccttctctc acgccatgcg tgagatcgaa
    46921 cgggcgaacc ccgacaccct gtaccgcgta tttggtgcag ccgattgggg caaccgcgaa
    46981 atgctgacgg acgggattct gaaggatctg atcgaagggc tttcggaagt gtcgctcggc
    47041 aatgccgcgg tgagtacgga catcctggga gatgcctacg agtatctgat cggcaagttc
    47101 gcggacgtta ccaagcggaa aaaggctgga gagttctaca cgccgcgcag cgtggtccgg
    47161 atgatggtga agattctgga cccgaagccc ggtgacgcca tctacgaccc ggcctgcggg
    47221 acgggcggca tgcttctcgg agccatcgag catgtgaaac agaacggcgg cgaccctcgc
    47281 acgttcttcg gcaaaattca cggccaggag aagaacctca cgacggccgc catcgcccgc
    47341 atgaatctcg tcctgcacgg aatcgaggac ttcaagatcg tgcgtgagga tactctccgc
    47401 agccccgctt tcaccgattc cagcacgggt gggctgtcta cgttcgactg tgtaatcgcc
    47461 aatcccccct tctccctcaa ggaatggggc cgggagatat gggaggccga tccatggggc
    47521 cgtgcggcct tcggtttgcc gccggacagc tacggtgact acgcctgggt ccagcacatg
    47581 gtggcgtcca tggcaactcg caccggcagg atggctgtgg tgctgccgca aggggcgctt
    47641 ttccgcaagg gtgcagaggg cgagatccgg cggcaactcc tggagcggga cctgatcgaa
    47701 gcggtgatcg gattggcgcc gaacatcttc tacggcacgg ggctggcgcc ggccgtggtg
    47761 atccttcgcc ggaccaagcc ggtcgcgcac aagggcaagg tcctgatcgt ggatgcctcc
    47821 agcctgttgc gcgaaggccg ggcgcagaac tacctggacc cggaacatgc cgagcagatc
    47881 gtcgcctggg tccgggcttt caagagcgtt gaagatcggg cgaaggtcgt gatcctggac
    47941 gagatcaagg cggaagactg gacgctgaat atctcgcgtt acgtcttgcc cccggtcgcc
    48001 gaagacatcc cgccgctgcc ggaggctgtg gcggcgctca aggctgcgct gacggaagcg
    48061 cgagcggccg aggataggct gcgcgaagtg ctgacgacgg gagggtggct gaaatagatt
    48121 gccttgcaga tgcaacattg ccttgcagat gcaatattgc cttgcagatg cataccgctc
    48181 ttggcactat tgggcagcag gaggtgagcc catgaacgat aagagcaaaa acaaccgagc
    48241 agcggacctt gtcagcgaca gcgccatcgt agaggtcaaa gcctctctta cggccgcgcg
    48301 ggggctgcgc gatgccctta cctctctggc cataacactg gcagagcagc agcaaaagcg
    48361 cgggtatctg ctcctcctcg atccgggact aagcaagact ttccttgaaa ccgaattgca
    48421 gcggctcaag gtcgccctgc gccccgggat tgccgagcga cttcatctcg ttgcagcaaa
    48481 aggaggaaaa cttaccgagg gaacacacgc catcccacag gatgattata tgctcctgcg
    48541 tcgcgcactg aataggcagg aagaccagcg aacgggcctc cccgccccca acaaacagaa
    48601 tgaggttttt ctgctcatga tccatcagtg ggtaaccggc aaaggcccca tgacatccaa
    48661 gtggctggag gacacggtgg ggtgtcacta tcgcaccgta gccaacgcca ttgaccagtt
    48721 ggggcatgcc gtccaaaggc attctgatag gagcgtgtcg tttaagtatt tccccgagca
    48781 ggactggggg cgtcttctgg ctgtcgcgca aaagacgcgg gcaacgatgc tgtacgcgga
    48841 cgcctccgac cagccgcggt cgccggaatc ccttctgcga cgaatgaaga gtctgaatag
    48901 gaaggacata gcggtcggtg gcgttctcgg gacgaaacgc tactacaatg aattggacat
    48961 cgttggaacg cctcgcctgg atttgtgcat ccattgcccg gacaatcgag ttgacctggg
    49021 ctttgttcac atgctcgatc ctgcacttga gcgcacccgc gatactcacc gcccggctcg
    49081 ccttgccatc cactttgttc ggcggcaaga cgccctcttt gacagggacc aaaatggtgc
    49141 gcagtgggcc gatccggtgg aatgcctgct ggagttgtac catgcccgcc ttgatcaaca
    49201 ggcgcgaagc ttccaggagt tcttgtcagc acgcgggaga gagttgagtg gcaaatcctg
    49261 aaaacaatat gcggttggtt gatcctgccg tggccctgca ggaaattgca gacgccgtgc
    49321 cgaccgcttg tcgcaagcat gtaattatca tcggcagtct cgccgctggc tactattacc
    49381 gtgaccaact caaaggcatg gccgtccgaa ccaaggatgc cgattgcttg ctatctcccc
    49441 gcgtggccgc tcttaaggcc gggattgcca tcgcagagga actgcgggat gccggctggg
    49501 agttcaaaaa gacaaagggc cataaggtgc caggaaccga ggatactcct gacgctgaac
    49561 tccccgccgt ccgcttgtct ccacccggca agatagaatg gttcatcgaa ttactcacgg
    49621 ttcctgagtc accgggggac cgcggccctc gctggacccg cctgaaaaca aagtatggcg
    49681 atttcgtcct gcccagcttc agattcctct cccttgcgaa tcttgacccc gtggccacgg
    49741 aaatgggcat ttcgatcgcc cggccagaga tgatggcgct tgcaaacctt ctggagcatc
    49801 ccgagatcaa gacggatacc atgtcgggcg gatttgcaga tcgaatggac atcaagcgaa
    49861 gcaacaagga tctcgggcgc gtgctggcga tagcccgcct tgctatgggg cgcgacgagg
    49921 acgcgctcca atcctggccg gcactctgga agacagccct tcaggaccgc tttcccgatg
    49981 agtggcgggt gctggctggt cgcgctggac agggcctgcg ggtcctgctt gcgaacgagc
    50041 aggaacagga ccttgagcag gcactttaca cctgcgccaa tggccttctc gcatccaaga
    50101 agccaacgct gacacagctt cgtattactg gcgggcgcct ccttcaggac gccatcaaac
    50161 cgctggaagc ggaggcccgc aaacgtgcct gaacgactga cacagcagga gcttgagtct
    50221 tacctctggg gcgcggccaa gttgctccgg ggcctggtgg atgcggggga ttacaagcag
    50281 tacatcttcc cgctgctgtt cttcaagcgg ttgtcggacg tgtgggacga ggagtacgcg
    50341 gccgcgctgg cggagacgaa ggacaagaag tatgcacggt cgatggcgga tgatcggttc
    50401 gctattcccg acggcgcgca ctgggcagat gtgcgcgggg cgtcgcgcga cgtgggacgg
    50461 gcgctgctaa cggcgttccg ggcgatcgag gcggcgaacc cggcgcggct ggccggcgtg
    50521 ttcgggaatg cctcctggac cgacaaggcg cagatgccgg acgagacgct caagaacctg
    50581 atcgagcatt tttcgcagca cacgctgagt ctcgcggccg tgccggaaga cgaactgggc
    50641 aacgggtacg agtacctgat caagcagttt gccgacgaca gcgggcacac ggcgcaggag
    50701 ttctacacca accgcacgct tgtccacctg atgacgcaga tgctcgaacc caaggccggc
    50761 gagacgatct atgatccgac ctgcggaacg ggcggcatgc tgatctccgc gctggcggaa
    50821 gtgaaacgac gcggcgggga cgctcgcacg ctggggttgt tcgggcagga gcttatcaac
    50881 atcacggcgt ccattgcccg gatgaacctg gtgctgcacg gcgtggagga tttcgagatc
    50941 gtggccggca atacgttgag cgggccggcg ttcatcgagc gggacaagtt gcggacgttc
    51001 aacgtagtcc tcgccaatcc cccgtactcg atcaagaaat ggaaccgcga agcgtgggcc
    51061 agcgatccct ggggccggaa cttcctcggc actcccccgc agggccgggc cgactatgcc
    51121 ttctttcagc acattctcaa aagcctggac ccgaagaccg ggcgttgtgc catccttttt
    51181 ccgcatggcg tgctcttccg caatgaggaa gcggagatgc ggcggaagct ggttgaactg
    51241 gacctgctgg agtgcgtcct cgggcttggg ccgaatctgt tctacaactc gccgatggag
    51301 gcatgcgtgg tcgtctgccg cagccggaag ccgccggcgc ggcggggcaa ggtgctgctc
    51361 attgacgccg tgaacgaggt cgcgcgggag cgggcgcaga gctttctcaa gccggagcat
    51421 caggcgcgga tactggcggc gtacaaggcg ttcaccgatg agccgggctt cgccaaggtg
    51481 gcggccacga aagagattct ggagaaggac ggcaacctct ccatcccccg ctatgtgcgg
    51541 ccggttgtga acggtaatgg aaacggcggc ggggatgcgc ggagcctcaa gaaggcgtgg
    51601 gcgacattcg aggccagcgg gcgcgagttc tgggaacaaa tggacgagtt ggtcgagact
    51661 ctggacggcg tcgtcgccgg ggaggcgacc gatgcctgag aaggtcaaga aggggtggac
    51721 gcgagtagcg ttcggggatg tggtccaact gtgccgggaa cgggccggcg atccgggtga
    51781 aggcggtttc gaccgctacg ttgggcttga acacctggag ccgggcgacc tgaagattcg
    51841 gcgttggggc aacgtggcgg acggcacgac cttcacgaac gtcttccggg ccggacaggt
    51901 cctgttcggc aagcggcgcg cctatcagcg caaggtcgct ctggccgatt tcgacggcgt
    51961 gtgttccggc gacatctatg tcttggaggc gaaaggcaac cggctgttgc cggagttgct
    52021 gccgttcatc tgccagacgg accgattctt cgagcacgct gttggcacgt cggcgggttc
    52081 gctctccccg cggaccaact gggaaagcct tgccgatttc gagttcgccc tgccgccgtt
    52141 ggaggagcag cggcggattg cggaggtcat tcaggcggct gcacgcttga ccgaacgatg
    52201 gcgggcggtg gtgcatcatc tggctagttt ggtttctgct ctggttgatg ttcctaacaa
    52261 cagggaactg caacgtctgc ccctcgttac gaaggacttg ggggaactgg ccgaaatcac
    52321 gaagttggca ggctttgagt acacgaagtt cgtgaagtac agaccagacg gggaggtcat
    52381 cgcaattcgg cccctcaaca tcaaaggggg caggctggtt ctggaggatg tccagaccat
    52441 tgacaggaga acctcaggct tgcttccccg gtcgaaagta aaagccggtg atgtgctgat
    52501 cacgtacatt ggggagtatg tcggtgaagt gctcctcatc gaggaaagcg agaagtatca
    52561 tctggcaccg aacatagctc gcattacgag tcatggcgcg attatgccac gcctcttgga
    52621 gcgtcttctt cgagggagcg atcttcaacg ccagattgca agtcgtacga cgaccacagc
    52681 gaccccgagc ctaacaatga gttcgcttcg gaaactccga atcacctttc catcgaatgt
    52741 cgaggatcag cggcaccttg tcgatcaagt caacgccgta gactccgcat ctgaggaggt
    52801 caagagtcgc ttggtcgctg cgcgcggatt ggagaagagg atatcggcgg agaccatgaa
    52861 cggagcgtga tgccatgcca ttcaacgaat ccaacaccgt ggaagccttc atccgcgacc
    52921 ggttggcacg cagcgcgcga atcccgcgac ctggcatggt cagcttgccc ggcccggtct
    52981 gggacgaggg cctggggtgg aagtacgcgg cgcccttgaa cgtgccgcgg cagccgcaag
    53041 atgtgctggt cgaatcctgg gtgcgcgagg cgttgatccg gctgaacccg gagatcgcgg
    53101 cgcagcgtga ccgggcagac gatgtgctgt acaagctccg ggccatcatc atgggcgtgc
    53161 ggagcgacgg gcttgtgaag gcgaacgagg agtttgccgc gtggctgaca ggcgagaggt
    53221 ccatgccgtt cggcaagagc ggcgagcatg tcacgatccg gcttctcgac ttcgacgagg
    53281 tggggcgtaa cgagttcgtc gtcacgacgc agttcacgta tcgggcaggc gcaaccgaga
    53341 agaggccgga tctggtgttg ctggcgaacg gcattccgct ggtggtcatc gagaccaaga
    53401 cgccggtgcg ggccagccaa agctggctgg acgccgcaat tgacatccac gaggactacg
    53461 agcggaacgt tccggagctg ttcgtgccga acctgctctc cgtggcgacg gagggtaagg
    53521 aactgcggta cgggtccatc gggctgccag tagaactctg gggtccttgg cgcgtggaag
    53581 ccgagatgcc ggcgccagcc ctgcaggcgg tctccaaggc ggttgactcc ctgctccggc
    53641 cgcatgtgct gctcgatctc ctggcgaact tcacctgcta tgcgacggac aagaagaagc
    53701 ggcggatcaa gatcgtggcc cgatatcagc agtacgacgg cgtgaacaag atagtcgagc
    53761 gcgtggtggc cggccagccg aagaaagggc tgatctggca cttccagggg tcgggcaagt
    53821 cgctcctgat gctttttgca gcgcggaaac tgcggctgca tccggcgctc aagaatccca
    53881 cggtcatcat cgtggtggac cgaattgacc tggacacgca gatttccagc acgttctacg
    53941 cagcggacac gcccaatctg atcaaggccg agagccggga ggaactgcaa cggctgctct
    54001 cgcaggacgt gaggaaaatc atcattacga cgatattcaa gttcggcgag gcggacgggg
    54061 tcctgaatcc gcgcagcaac atcattgcga tggtggacga ggcgcaccgt acacaggaag
    54121 gcgacctggg ccggaagatg cgacaatcgt tgccgaatgc ctttctcttc ggcctgacgg
    54181 gcacgccgat caaccgggcg gatcggaaca cgttctacgc ctttggggcg gatgaggaca
    54241 aagccgggta catgagccgg tacgggttcg aggagtccat ccgggacggg gcgaccaagc
    54301 cgctgcactt cgagccacgg ctgctggagc tgcacattga ccgggaagcg attgacgaag
    54361 cgtaccggga gttgactggc ggattgagcg acttggaccg ggaccgactg gctcagcaag
    54421 cggcgcggat ggctgtgctg gtcaagtcgc cggagcggat cgagaggatt tgcgcggaca
    54481 ttgcccggca ctttcaggag aaggtcgcgc cgaacgggtt cggagcacaa gttgtgactt
    54541 ttgaccggga gagttgcgtg ctctacaaga aggcgctgga tcagcacctg cctccggaag
    54601 cgtccgacat cgtgatgacg gtgaacagcg gggaaacgga atatgcggcg tatgaccggg
    54661 accgggatgc ggaagagaag ctgttggacc ggttccggga tccggccgac ccgctcaagg
    54721 tgctgatcgt cacgtcgaag ctcctgaccg ggttcgacgc gcccattctg caagccatgt
    54781 acctggacaa gccgttgcgc gaccacaccc tcctccaggc gatctgccgc acgaaccgcc
    54841 cctacggcga agcgaagacg catgggctga tcgtggacta cctcggtgtg ttcgatgacg
    54901 tggcgcaggc gatccagttc gacgaggaag gcattacacg ggtcgtcaag aacatcaacg
    54961 aactcaaggc ccagttaccg ccggccgttc agaagtgttt gtcctatttc gccggcgtag
    55021 atcgaacggt cacggggtac gaggggttga tcgcggcaca gaactgcctg ccgaacaata
    55081 atacgcggga taggttcgcg gccgacttca gctaccttag ccgattgtgg gaagcgatct
    55141 ccccggaccc gggtttaaac ccgtacgagg acgactaccg atggctctcg caggtctacg
    55201 actcggtgaa gccctcttcc ggcagcggcc ggttgctctg gcacgcgctg ggtgcgaaga
    55261 cgatagagtt gatccaccag catgttcaca ttgacgcaat ccgggatgac ttggagaccc
    55321 tcgtccttga cgctgacctc ctggaagccg tcctgggcac gccagatccg aacaacaagg
    55381 ccaaggaaat cgaaatcaag gtatcgcggt ggctgcggaa gagactgcat gatccgcggt
    55441 tcaaggccct tgggcagcgt ttggaggagt taaaggagcg gcatgagcag gggctcctga
    55501 ccagcgtgga gttcctgaag gaattgctca atctggcgcg cgaagtggtg acggccgagc
    55561 gggcgaagcc accggttgag cgcgaaattc agggcaaggc cgccttgacc gagcttttcg
    55621 aggaggtgaa gaacccggat acgccgatca tcgtcgagcg cgtcgtgaac gacatcgacg
    55681 aaatcgtgcg gtacatccgt ttcgacggct ggcagaacac ccacgccggc gagcgtgaag
    55741 tgaaaatggc gctacggaaa acgctgttca agtaccggct tcaccaggac caggaacttt
    55801 tcgaccgggc atatgggtat atccgtgagt actattgatc gttcacgcga gctgctgaag
    55861 cggcacggcg cagcgcgagt cctcccgcca ggccgcacct actccagtca cttttttaaa
    55921 cagcgcgttt tcgctggcaa catgagtact gaagcgagag actcgtcatg ctttaaagac
    55981 aagttgcctc cacttcaggc caatgctgcc gcccttgagg cgataacggc acacatttat
    56041 taccttcgag gcaggacgac tggcagaccc tacaagtgcc ggttttttca tgtgacttca
    56101 atcccatgag ctcttttccg aagctacaac ttgatccgcc tactgactgg ccctcattcg
    56161 aggacctctg ctgggggctg tggaagacca tctggcgcga tgaaaacacc cagaagaacg
    56221 ggcgtggcag tcaaccgcaa cacggtgtcg atgtttttgg acgtcccggc aaggcttcga
    56281 cgtgggctgg agtacagtgc aaagggaagg aggtcttcac gcaccaggcg ctcacgccaa
    56341 aagagattgg tcgcgaagtc aagaaggcaa ggaagttcaa gcctccgttg tctgagttca
    56401 tgattgcaac gacggctccg cgtgacgcca gcttgcagga attggctcgt aaagtaacag
    56461 cgcggcatgc caagaggcac tggttctctc tgactgtgct tggttggcac gacattgtgg
    56521 aactcctact tgagtacgaa ccgcccattg ccagtcggtt ttatcccctt gtattcggag
    56581 aaggagtgaa aggagtcctc tggaaaattg agcaacgcct gaccgagtcg cctgaccgtg
    56641 attcaatagc tgctgatgta ccgtactatg cggctgacga ttcaccatgc cacagccagg
    56701 acttgttaca gacatcacag gactcaacga ttacggtcag tgtcacgatg gagcagccga
    56761 atcctggcaa ccctattcgg gcgagggatg ctttgaaggc cctatcgctt ctggcccaga
    56821 tacaagaaat ctgcctcgcg caggccgaca agaatggcgc cacatcgttt gacgcaacac
    56881 ttacttcggc tattgaccaa gccaaggcac gacgaaactc acgggataaa gcggatggcg
    56941 gagcgcaatg aacgccaaac ccacatccgg cgtgactctc atggatgttc cgattctttt
    57001 aacacgggta agacacgaac tcacggccgg gaactacagc gcggcggtta cgctcgcaac
    57061 agagattgaa gcggttcttc aagtcaatga gtcgtcgttg cagaaggtcg cagccgatgt
    57121 tcttcttctg ctggcgcaag cacacgttac gcaggcagag acagcaccac aagacagtac
    57181 tccacacata aagaaggcca acgagttgat cggcaaggcc gagcgaatgt tggaacaagg
    57241 tgcggatacc atcgataaag acatcgctct cagggccgca attgctgctg tcgaagaagg
    57301 agccgaagct ggtttacttc tcttacaggg gcgggccgac cccttcgcga tacgaacacg
    57361 cactgcgtta cttctgaatc agcagaagta cagcgatgca atggcaaccg ttgaaggctc
    57421 ggcgccacat gagtggtggt gcgatgtggc ggtcatggcc tacgccctca atgacaaggt
    57481 agaaaaagct caagagcttg tccgctgggc ggccggcttg tcggatcgga gccgttatct
    57541 ccaatgctgt gtgcggttgg ccgagggcat tctgggacag gtcgttcaag cgcatccgaa
    57601 ggacgtgaac atcctgtcac agaccctaaa tgcgcccgaa cgcgagaaac tcgtggttgc
    57661 agtagaggcc cttggcccca tccttcaacc gattcaggcc gccagaaaac cctcgtccgg
    57721 cctggacatg tccgcgttgc ggatcgcctg gcaggcaaac tatcttctcc agaaccggga
    57781 ggccgctgca gaagcgctta aactaatggc ccagtggact ccggttccct tggatgtggc
    57841 tcgcgggggg atgaccggct acatcgaagc gccggccgac ctgcccgacc gtttgcggcg
    57901 cgatcatcct cgcgatctcg atgcgaacat tctggcggcg atcatcgaat ccgcttcctt
    57961 cggtcgccac aaagtagcgt ttgcacaggt aaaggatctt gtcccgctgg cggatacgga
    58021 cgcaaagaag gaagaactct tcaagctgct gcaaacgcta tggcaaagtc ttgaaggccc
    58081 ggaagccgcc gaatgcgaga cgattgccaa accgctcgtt acccacaatc cgcgactccg
    58141 cacaatcttt gaggcatccg tggccctgcg ccgaggcgat gcagataccg ccgtggcctt
    58201 gttggacggg caaaaggcga cggatgatct cagctggcta cagatgcgcg cgaatgtatg
    58261 tctccagaaa cgacagtttg ctgatgccgt tgatttcctc ttaccggcgg cagagaagac
    58321 gctcgatccc ggattgctgc acaagacggg ggattttgca tttcaagcta agcgctatga
    58381 cacggccgct tggtgctatg agaagctggt agagcgtcag ccgaataacc tcatggtccg
    58441 cggcaacctg gctcacatct acacctttgt tctgcatgat ctagagaaag cggcgggcca
    58501 attccaagcg ctgcgggcgc tagaacccaa caacccgacg catacattaa acctagccgt
    58561 ctgcctggcc cagctgttcc ggcccgagga gagccttgcg ttatacgatg aactatgccg
    58621 tcttgaaaat ccgccgctcc ccacggtcat ggggcgcgca caattgcatc atagcatggg
    58681 acaccccgac gcagcgctga aggcgatgga cccattccgt tccaggttct ggggggaatc
    58741 caccttcttg ctggcgtaca tgggtacggc ctatgcagct ggtcgcgaag atgccgccca
    58801 cgaggcgctg gtcgccctga acgaaatgca gcaggccggc gccgtcaagc cggaggcgtt
    58861 ccgtgccgtt cccaaggatg aagcgttgga aaatttcaag cagtccttca agcaggcaaa
    58921 ggaccgcaac gaatacctgc acgccgaaat gcttaaaggg cgaatgccgt gggtgtgggc
    58981 agatcaggta gcgaacaacg ccatttactg gggctggcgg atacggaccc agaaaatgga
    59041 ctggatcggc gacgaaccga cgaaccgggc gcgatattgc atctacgcaa ccaatggctt
    59101 ccatgcccgc gagtccgagc gcgggcgtcg cgaacttctg ccgctggagt gtcccccggc
    59161 cggtacgcga atcataacgg atatttcagc tctgatcact ctgcaccgcc ttgacctgct
    59221 ggatactgtt ataaaccatt tcggagaaat gcttgtgccc gcgggctacc tcccgacagt
    59281 tctggaggac agccggcgga tggttcttaa ccaacggacg cagcaacaga acgccgaaca
    59341 gattacgaag cagataaacg ccgggcgcgt taccgtattg gccgagcacg agaatcctgc
    59401 ggcaggtatg ctggtcgtgg acgagtacgg ggagacgacg gaacaccggt atcgccttac
    59461 ggacgtgatc cagcctttgt atgccgcagg aatcgtcacc gatgccaatc tagaccgcat
    59521 ctcccgcgtg agcgcgaagc cgaccgcagt ggacgactca catccggctt tgagtcggtt
    59581 ccaggacgtg ctggtggacc tgaccacatt ggagacgatc acaaacgccg ggctgctcga
    59641 cgctgtcgct agtttttacg ggctccggat cacggcggcc gcccatcgcg aagtgattca
    59701 gcgcctgaat gccattgcct gccaggaaga aacgcgccag tggcacatgg acctgtggaa
    59761 gcggctccgg gacgatcccc ggttcaagtt tgtaccgcat accgtaccca aggcaatgcg
    59821 ggacaaagat agcaaggaaa gagattacct cccgtttctg gccagtttca tcgcacagga
    59881 gacgaagacg cctctgctgg ttgacgatcg agcctgtcag gctttcacgc tgaacgaatc
    59941 agcggacatc ctctgcgcag cgtttgggtc ggatgccctg gttcttcgcc tcatggccga
    60001 aggcaaactg gacgccggca agactgcgga tgcgctaaaa cagcttctgg cttggcgcta
    60061 ccgcttcctt gtgctgcccc cggcaattct aaagatactg gccgacatgc accggggaaa
    60121 gccgccagga caagcattgc gagaagtggc ggggtacgtc cacgactgca tgcgcgactc
    60181 ggggttgttc ggtgggccgg aaaagaccga attgggcgat tcgatggcca tgcgcacata
    60241 cctgacttgg gtctccagta tcgccgagtt ccttgtgctc gtgtgggccg acgagggctt
    60301 tgcaccggaa aaggcgacgc gcctgacaac ctgggcctgc gcggaactcc tgccggcccc
    60361 tccccgagtg gttgacgggc ggatgaaagt gcagatcgcg gaaatgactc cgcggttatt
    60421 cctgtcgcat gccctgctga agacggcaaa ccactccggc gagccgcgaa tggccgacgc
    60481 catgaaagcg atgaaagacg gcctgcgcct gacggacgac gagtatcatc gagtcgtatt
    60541 ggaaatccta aatgacacag caggaacagc tccccagccc tgaagagatg agcaaggtgt
    60601 t
//
LOCUS       DAOOTK010000007        57233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_438_length_57233_cov_6.423415, whole genome shotgun sequence.
ACCESSION   DAOOTK010000007 DAOOTK010000000
VERSION     DAOOTK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 57233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57233
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_57233_cov_6.423415"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PK634_01805"
     CDS             <1..689
                     /locus_tag="PK634_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96501.1"
                     /translation="QRCAAELVPREVRQADLPCPLDQLPARHGEEIARILRAETGPLS
                     AQEVADAVSVQLSYGPDDLTLIDWNAALIFDRDAGDVLDVLEFANIELLELRVLDAQL
                     DDSLDRSYELLSRFTWQRLLGLSRMSSLRRVGQMQVDGAILFERVSNALKLLGDQYLA
                     RVYRLASQRFHLAEWNAGNLRKLDTIESLYEKLSDRNANRRLEILEWIIIALIAFEIV
                     WPFVSKRWGL"
     gene            686..1072
                     /locus_tag="PK634_01810"
     CDS             686..1072
                     /locus_tag="PK634_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOW96502.1"
                     /translation="MKTLARRLFYWLPPLAWAGAIFYLSSLTRLPRPPAYPGEDKVIH
                     ALFFGILALWLLRALAGGQGMSAGRAAAFAFAFASLYGAMDEVHQHFVPPRTMDAVDW
                     LADTFGAAVVFLAARCLPRAEGAAGP"
     gene            1069..3132
                     /gene="ligA"
                     /locus_tag="PK634_01815"
     CDS             1069..3132
                     /gene="ligA"
                     /locus_tag="PK634_01815"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOW96503.1"
                     /translation="MTRTQAEKRVAELRRALNRHNRLYYVEARPEITDHEYDALYKEL
                     RVIEEQFPGLVTPDSPTRRVGDEPLKEFRNVRHLVPMMSLDNTYDFDELREFDGRARK
                     LLPGEPIEYVLEPKVDGVSISLRYENGAFTLGATRGDGTTGDDITANLRTIRSLPLSL
                     ALAPSPSPSPSPSPSPSPPPLLEVRGEAYLPVEGFKKLNAQREKDGEELFANPRNACA
                     GSLKQLDSRIVAARPLSAVFYAVGACEGIEFAEQAEVLEALKAFGLPTPQFWRRCRDI
                     EEVIAAAGELQKLEATLPYEIDGAVVKVNSLAQWKKLGTTAKAPRFAIAYKYSHEQAR
                     TKLKAITVQVGRTGTLTPVAELEPVFLAGSTISRATLHNEEEIRRKDIRVGDTVIIEK
                     AGEVIPAVVAVVPEERPRGAKPFDLARHVAGKCPACGGPIRRDPEFVAWRCENIACPM
                     QLKRTILHYASRRAMDIENLGEVLVNQLVDQGLVKDVADLYGLAAEPLAALERMAEKS
                     AANVVEAVAASKGRELWRLINGLGILHVGEGAARKLAAQFRDLDALAAESVEGLQQAR
                     DVGPVMAESVHDFFRNPRNRSVIEKLRKAGVNFKTGPESEAPAAGGPLAGKTVVVTGT
                     LKNYSREGIKDRLHEAGATVTDSVSKKTDYLVVGADAGSKLDKAKKLGVTILDEAALE
                     KMLAG"
     gene            complement(3210..3842)
                     /locus_tag="PK634_01820"
     CDS             complement(3210..3842)
                     /locus_tag="PK634_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW96504.1"
                     /translation="MNTNRRDFDKEAANWDENPARVRLAADVARAIARQVPLTPDMDI
                     LDFGCGTGLVALNFAPLVKSVTGADSSLAMIEVMEAKAREQGLNNVHTRHLLPGGSLA
                     GAYDLIVSSMAFHHIEQPAALLRQLFAALKMPGRLCVADLDSDGGEFHHDNTGVFHFG
                     FERNALGDWFRQAGFSKITATTAAEVTRPGRTGAVRTFSVFLVCGEKNAG"
     gene            complement(3855..4196)
                     /locus_tag="PK634_01825"
     CDS             complement(3855..4196)
                     /locus_tag="PK634_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOW96505.1"
                     /translation="MKNISGRRIASARKRNNNWITQQELSDAAIARGVQLDRAAVAKI
                     ENGLRGVLDYELLTISEILGVELDWLLSERATPAAAEPAAPPHPDELEEPAPSRPQPP
                     ARDENRALIFL"
     gene            complement(4286..5614)
                     /locus_tag="PK634_01830"
     CDS             complement(4286..5614)
                     /locus_tag="PK634_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW96506.1"
                     /translation="MRITIREKGTILLLELDGRLDAFGSSELAGALKQDFDSATVCLL
                     LDMSRVDYISSAGLRILLPVHKELMRRDGALILARLQGYCREVLEVTGVADVLPQFDN
                     LEKALASARRAALRRHNRDHWDHLETAALDHGAVRIMNAGGGPGAVEVLGDVRDVLHA
                     RVTPDQLLSKRFFETEYSIGLGGLGRAPEDFFEIMGEMMTIGGTMVWLPTDGHDTPDF
                     LIPKTDKGHVMIRTGYNISLAGGFDEFMVFESREPSGTTISQLYRDLFQLARRRRPDC
                     KGLLGLALRAQAVAVYGSGVLRSPVAPLAPANGGLITDPSNIGEWMACDREPRHRNVT
                     MLMCGVGADLAMPLDAFDSVELNKVFYLHPANIGAKTELLHNHAVLFKEQPFPERPTD
                     LEDEIQRVVQNGEFVDMRHLLDNSCITRALIGVSYIQTVRPDPHHDPSPA"
     gene            6033..8585
                     /locus_tag="PK634_01835"
     CDS             6033..8585
                     /locus_tag="PK634_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOW96507.1"
                     /translation="MGGWLKGALTGAVLVTALWLLAPQRSIQHEDGAVEITFMGPDGP
                     IKGALEDSIREFERLSRLRHEQDPSYPVYRVISGQHASRNQVEDPTRFIVSVAGGMPP
                     DVIFFDRFAISEWAARGAFAPLDDRLARDEEAWAAWQADPSKPEPWPGAREEPPRAKG
                     ASALAAITPIRADDFFPACWDEAVYRNPLTGEQGTFGIPNNADNRVLLYNKDILIRHG
                     FTNELGEAQPPRTWEELEEMAIAMTERDAAGKIKTIGFIPNYGNSWLYLYGWQAGGEF
                     MSAEGRTCTLNHPRVVEALDFMTRIYDRLGGASDVYAFQTTFQGDALDPFIQGKVAMK
                     IDGVWILSSLAFYGRDLDFGSAPPPMPKRELDAGRAPISWLAGWAYAIPSTARQPDGA
                     WELIRYLVSQRSLAIMLESEYFTYASQGRPYVPRQYPNRAQNEYAFERYVQNNPNMEP
                     KFKAAMKQFNDLLTNSRYRPVTPVGQKLWNAQIWAMEDAIFKKKTAKESLDYYTAIVQ
                     RDLNLLLDPPKGRPVRSWAWFFWVYGALVVLAVVAAWWRDTRRKGLEGSRSAYFRAEW
                     RGGVACALPYLVGLVVFTGGPLLFSIVISFCRFDVLNPAVFTGLRNYLFMLRGDELFW
                     KSLYNTVYMVLGIPLGMALGLGIALLLNLKVRGVAVWRTFFYLPSIVPAVASSILWMW
                     MFNPQSGLLNAALSAVGIEGPNWLQDEHTSKGALILMGLWGAGGGMIIWLAGLKGISE
                     SYYEAAAIDGAGTWHKFVYITLPMLTPYIFFNLVMGLIGTFQIFTQAFIMTAGGPVNS
                     TLFYAYHLFNNAFRYLNMGYAAAMAWFLFLIVVVLTAIQMKISSKWVHYEGE"
     gene            8608..10269
                     /locus_tag="PK634_01840"
     CDS             8608..10269
                     /locus_tag="PK634_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOW96508.1"
                     /translation="MNESAQSDLIRRRLAGLSRALLIYGLLVGGAAVFALPFVWMIGT
                     SFKVDREIFGGKITIFPMRPDPVAASPYMDARYYREPTDADYPRVKPVIEQVLAESGA
                     GDADILAPGLHERLKQVLPDAAWKGTDGELGAAARARLTPELIEDVRDNVARALLLGP
                     VRVRSFDLQEADLTEGAPVSAFWTVESPVVARLVDETNATPPAAVLNYDFGDQRDASI
                     TLTTTLDLPFAADRLHRVTLFLRPDDSWHPLDFTLEKGGTLYRGKRPEYLGNFNWGSV
                     TLQEAGPDDESTKIRLWLPYEKVDAGSQYESRPDKAKLTVILRRNTQAGAWWAKVQRN
                     YRGALDYLPFWRYAATSVFLVILNIVGNVFSCSLVAYAFARLRWPGRDLTFGVMLATM
                     MIPPQVTMIPYFLIIKSLGWYNTLTPLWFVSFFGNAFNIFLLRQFMKGIPRDLEDAAK
                     IDGCNVWQIYWNVILPLIKPTLACIAIFTFMGVWNDFMGPLIYLSDQRLYPLSLGLYA
                     FNVQAGGNYGMMMAGSLMMTLPVVVIFFFAQKYFIQGVTLTGMKG"
     gene            complement(10371..12488)
                     /locus_tag="PK634_01845"
     CDS             complement(10371..12488)
                     /locus_tag="PK634_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96509.1"
                     /translation="MVGCEIARVAWATDGENRGIVMTTHQHKEPRFLDNRLKTAEREW
                     IRTRREQVNADPSGARHRIPYAHPETPGQDVGLALSGGGIRSATFNLGLLQALQRYGH
                     FWKIDFLSTVSGGGYIGSCLTWFMSCLGMEFPLGARRSDNAEFPGAVHNWLRDHGQYI
                     TPGRGMGAFAVAAAVLTGLLVNLAILLPPALLLIQALRVPIGSRPASIWIGIGGAILL
                     SLLVAGMFAQALLSGLTRSSPWFQRLECQCEKWKGRLLLFGLCGLLVSIVPWIHDLLS
                     CKGAAWIRSIKIPAGLSAGGVCSILVALIGGKSKSSALSPGRSFFLCAGLSLLIYALA
                     ITLFSWAGRVFQPAMPFWIFPAVFTAALLLGLLADINRMSLHRFYRNRLRDAYLPRPR
                     LLVPEPERAQYPDADPENSLLKDIQPTRAPYHIINAAQNTVGSKRYKLRERGADNFIF
                     TAKYCGSESAGYASTSEYGLGRTNLATAMAVSGAAVDPHTYATRSRALSFIMSFLNIR
                     LGYWIHRPGHPPPRWNTFWRPLWYRCMGYEMTGKGLSEDSVYLHLSDGGQFENLGLYE
                     LIRRQCRCIIVSDATADAERGFSDLGRAMELVRADFGTRIDINTAPLRIPKALGFYPA
                     CHVIGRIRYADESTGALIYIKPTLMKDTPEDVLAYHRKHPQFPAESTGDQFFSEQQFE
                     AYRELGFQTGRQLADVLEEELDL"
     gene            complement(12470..12844)
                     /locus_tag="PK634_01850"
     CDS             complement(12470..12844)
                     /locus_tag="PK634_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96510.1"
                     /translation="MNRLWRSLAVGVLALLAGLATSCMTRQAVTRAVEGWYVPDPDQV
                     LTVNGEWAMDGLHALFHVDDPPRDGTLYTNTAGDSLVFSRTNGIRVATIRGKNRQKTE
                     YEFLGYGGPNRSAVIGVWWDVK"
     gene            complement(12841..13590)
                     /locus_tag="PK634_01855"
     CDS             complement(12841..13590)
                     /locus_tag="PK634_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96511.1"
                     /translation="MSDQHHPIVAVHGVGSGTNWNRAGFSRELSDHVSRVQRPVRRIE
                     YGEEKPPRRPPCSIFWKEALWEGENDRIDKRVAAGLRLLSVKFPGAWLAAPLLDLLAD
                     VPLYCLTPQRRKIRKVVRDTIRMFPGCVVVAHSLGSVIVADILCQALRDGTLRRGDVS
                     ALVTIGSPLNILGLTKGAKMSGEFPFPWHDLYYPADRIHLKAPLDGKRFPGVDSRPLA
                     RDQGFVQAHTSYWGLSLIAKLVRRLSTGGAP"
     gene            complement(13709..14056)
                     /locus_tag="PK634_01860"
     CDS             complement(13709..14056)
                     /locus_tag="PK634_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96512.1"
                     /translation="MPLTAPDLERFQEASSRATRELEAESPGLPYAVAASRAARRAIA
                     AAGYDPDRTVAAWLSPSFTDDLATRRAADNLLSVILGDPAGAEDFEQILQELSPALQD
                     AARERRARHNGRP"
     gene            complement(14270..18565)
                     /locus_tag="PK634_01865"
     CDS             complement(14270..18565)
                     /locus_tag="PK634_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOW96513.1"
                     /translation="MSSKNRSMDIRVEAFSRRRVLLSLAAASLLLAPFSISAAATTAG
                     NVTGLTLTTNSPSGNITASFALSGGGSVEVTPFAADVVRVDYHWVGPFETDQPMIAKP
                     LADWTSAGATISDQGSKYVIQTSQLDIEVEKTPVKIHFKDKSGFYLLQDDFMEYDAGY
                     NYTGQRGTGSSKLKCQKIMPGGQAYFGLGEYGGPMNRRGREIDCWNTGTFNWGEYQNP
                     TYMNIPFFYGVQPAAGGNPAFVYGVFFNNPCRPLFKFNVSSETRYSFEAGDGRMDYFF
                     IGGGASHTMPSVINRFSELTGRPTMLPKWGLGHHLSRFSYDNQAWVEYIANQATVEDI
                     PLDAVYLDLDYMDANADGNIGDGQIRQLAFNSRYADPAGMVNYCNARGVKVVPLIEPW
                     LQPGDTAHYTDANNNMHFIKDNGGSTVTRNIYTGAVSWFDYSSTPMNTWWKDRIVSWF
                     NSIAFSGIWNDLTEPEGGDGIPYNGLLWVDGKFGTSSTDSRRWWSNERNYFGLRCARQ
                     SYNTMLAKDSNKRPFVLSRSGNAGLQRYGVSWSGDTAANWFYQRATIRFGMGAMISGA
                     AWYGNDVGGFSGTPSAELMVRSTEFNCLTPFFRNHANDNSADREPWRFSEPYKSQLRD
                     LIKLRYRLMPYLYTLAYQSTQTGEPMNVPPVFDYSADANTYGLSDYEFLVGDFILSAP
                     IYNEGASTRTVYLPYAPGVEWYYWPSGSPNSSPSGDKYAGGQTITVSAPLGKAPLFVR
                     SGAIIPMGPSMQYANQSQAAWMDIHCWPSGTSEFTLHEDDGETWDYLGGEYSRRRMVS
                     ARTAAAWDFTIEARQGTYNTGARDFYVYGYNPGTSVVHGVTLNGAPLAQLANFGAGSQ
                     GWLITGEGRIGIKLPDTGAAAALRVSFGDVNDTIQLTAATNSAAENAGSVRVYASRAG
                     AATGAVSVAFATANGTAVAGSDYTATNGTLHWAAGDLANKFVDVVLADDGSYEGHETF
                     SIGLSGAVGADLDSPGQQTVTILDNEPLPPDLLVTNPPTAIVVAEGTTNFTVQGVANA
                     LNWAGLAWSNQLTGATGTGAIGFSWSLAGVGLGIGTNRIVLSATNAGSAVLATDDGTN
                     AVYNDAWDLSDNGGSGWGAWQFYASSANSSENGRFMASAASVNIGAPAWGLYANSGNL
                     SEAKRLLTNALAVGQTVSVRMDNGSINTGSGAGVALQNAAGTTLWEFFFNGGDTFYSM
                     SSTTTDVGWTSTGLDVEFTLTSPTTFRARITPYGSNTRTNTGSLVTAADQSIAVFRAW
                     NYNAGAGSDYDFFFNRLTRLGAAGSGPSTSVVVEIVREGATSQIPQDWRDRYQLTGAG
                     SGDNDDRDGDGFSNLQEYWSDTHPTNGGSFFARLGVAGAGGPGAGPFTVEIGAPTTNS
                     RLYDVSYCSNLVEGTWTPLGYNRPGQADGGALSIAVTNALGTPVHFRSRVFLP"
     gene            18802..21408
                     /locus_tag="PK634_01870"
     CDS             18802..21408
                     /locus_tag="PK634_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96514.1"
                     /translation="MEKQKPAQKVAFILICLGLMMRASAAVTPTLTLWSSERRTVNTA
                     VDRIDTNRLYVATRAVEVSGNVQDPGGGEAVVVEDLLVRMVLPAGVALSEADDLEAAP
                     GMYALWEPAQTALVTRTAGPLTNGVSFRITALLVDSNAWLLPPVDVSRACDVARIGAL
                     GVITQSFVVTLGIAPGSLAGCESVEVSLPDWPYQQAPVQVSLAGSAWPSAFEQDLWGA
                     LPSYHATNPASLGGFYTFTNVLTVSNTTTETVDFMPQAEARCVERVASLGAARRGFVQ
                     LGVSNGPISVISAPHAVIWDGQWRQGETVLTLAAKWSTDVPALTDIKCVRAATIAVNG
                     LDMQELMFSAEGANLMGGLLVAPGGETWDMEPEGGEGLYFEPGSRHAADLAGFTNGSY
                     RIDLLGFTNGVAVSYAFDLRGGSVTQAPAFRSPGPFTNQPRPLVSWPGATPTNVSAVL
                     LILNNAWQDSEEFQMWPGAGQTNHVPENDLFAAIPCSLMYADVLSGSSGGASLLSGYV
                     SQRRGLFNVLTNEAIPAAVHLAAEPAATVVDQPVALGVVNGGLFPGAHVAFQLFFGDG
                     GQTNAPYAQRSYAAAGIYTARVVAADELGIAATGFVAVAVYPSPAFDTATRTGALLDL
                     SLFAVSGAVYRAYCADSLRPQDWMPMAMVLSGTGGPMRVHTALSTARRFYRLESSMPE
                     SGGNVGFEPLEFPVRRTGDIIRVAAFGIPNWSGTDPHNGIDLEVNHGLASSEIVSPVT
                     GVVERIEASETPFSEPPGQLMVTVTIRTPLGTDVSLVLEPSTADPVVKTNQLNAVHVS
                     EGQGVGVGTPVADLIVGALGYPHLHYMVMRGDEFVCPYGYSSPAAQAIFESLAGLPGS
                     SLPDGNICYGEP"
     gene            21504..22667
                     /gene="lpxK"
                     /locus_tag="PK634_01875"
     CDS             21504..22667
                     /gene="lpxK"
                     /locus_tag="PK634_01875"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOW96515.1"
                     /translation="MPDRRMEGLENYLLTVIEDRRPGRFAALLRFGLKQLSLVFATIV
                     QVRLWLYARGLFRHHTLGCQVISVGNLTVGGTGKTPVVEIFARSLQRQGRNVAILSRG
                     YKRDKPPFLTRLARRLSFRKDADPPLVVSDGQRLLLDSTTGGDEPYLLASNLPNVKVL
                     VDKDRVKAGRYAIEKLGCDTLLLDDGFQYLRLKHWADIVLVDRTNPFGNRRLLPRGIL
                     REPIRNIRRASFIFITKSIGDGAPDLRKALRELNPKAEIIECRHSPKYLRDVFTREKK
                     GLEFLRDLEIGAVSGIAAPKGFEDELVRLGAKVIYHKRYADHHRYTQQELLDAINRSL
                     QRGARAIVTTEKDAVRFPMIERRDLPIYFLRVEIELLSGEEDFHACISRICFK"
     gene            22636..23652
                     /gene="waaF"
                     /locus_tag="PK634_01880"
     CDS             22636..23652
                     /gene="waaF"
                     /locus_tag="PK634_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOW96516.1"
                     /translation="MPASPASASSDPGATLVCAPGWVGDGIMAMPALQELRRRNPDSR
                     IVLLVKPAMRPLWELHRAADALLEWPSGLRPAVTAVSAAGCERAVILPNSFRSALVPC
                     LAGIPERRGRPGHWRRWLLTDVVEPSGDPARAHQVYEYLDALVPGVPTGGFEAPRLAV
                     PPAAREKARERLAGLPAPRVALLPGAARGPSKQWPAGHFIELGRRLAERPGAGLVVLG
                     SARETELCGRVASGIGAAARSLAGETSLADWAALLEACDLVVCNDSGGAHVASAVGRP
                     EVVIFGMTDPSRTAPLGDRRRIIRAEGPCSRDIARHDPEAIKKLASITPEQVYQAALE
                     CWAG"
     gene            23721..24488
                     /locus_tag="PK634_01885"
     CDS             23721..24488
                     /locus_tag="PK634_01885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW96517.1"
                     /translation="MREQISACITACDEEQNIRRCLESVKWCDEIVVVDSFSKDRTID
                     ICREYTDRVVQHEWLGYIGQKNLIRGMARHPWVLFVDADEEVSPALRDEIQSLFGAGA
                     GAFAGFQFPRMVYYLGRWIRHGEWYPDIKLRLFRKDLGRSEGQEPHDRVAVNGPVKTL
                     KAPLHHYTYNDIWEHLETMNRFSTITAQEKYRLGERFRWREFLFRPGWRFIKAYFLKG
                     GLLDGPPGLVIALISMFGVYMKYAKLWELEHRKGGAA"
     gene            24485..26626
                     /locus_tag="PK634_01890"
     CDS             24485..26626
                     /locus_tag="PK634_01890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOW96518.1"
                     /translation="MTAPRATRIRATLAVLAYLAGTFLAAAVTAVALVRLMPHAPEEM
                     ARAVLRKGPDKVMSRCLQVFLVLLLPWLLRRLGWRGWHDLGYSAEDRPAARPGLSQLG
                     RGALFGLLTMGAVALAMSAAGARVYVADHGWNLNPLMLVVYAGAALAVGFFEETLARG
                     ILYRVPARIWGALPAALVGSVLFGLAHFVETDPAAFERAGFWPAVAAVLKSTRALPAF
                     SVAEGVRLANLALLGGVLCAFVARTGTIWFAIGAHSGWVFTIRLNGLLTTRVPESGGG
                     WWNGLRADGTDSAWTMLIMASMLAAAAWWPRRRARASAAAGTVRRDLPWERWAWALLL
                     AAAFGVPFSIALAQVCGGLGLACAGLAAIRGRLRIAWPLLFWPALAFAAIAVLSVVLG
                     PEPFRLVKKTGRLLWFLLIPVTAALAPTVPRRTALLKAFAAGSGVLGLKIVLLNSWQA
                     WRAQTVVLAGIPDYFGRMVALGSMTAGQMLMIGLLATAALFLWRRRRGGIPAGWWALA
                     AAQAAGLVLNFKRGSWITAVGLGAVSLAARRRWLWLLGLLALVGAALCLHPVRGRVEQ
                     LQVEWDTQGGGRLTMWTRIAPALVEQYPLGVGYRSVTAPLLMRIDPGVERKRNHLHSN
                     PVQVLVETGWIGLAVYLAWMGLGLAEAARRLRAAPMDGERRAQAVAFTWMLAGLLLNG
                     LVEYNFGDTELMIVLAIVLGALSADPAEDPA"
     gene            26623..28824
                     /locus_tag="PK634_01895"
     CDS             26623..28824
                     /locus_tag="PK634_01895"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOW96519.1"
                     /translation="MKLSPAPRVRPSRYSGHLDPDFWEPGLPEKMESLPALLAEGETV
                     QVGGRSRVVRLVLFRKRRPVEAAVKSFGRASLLKNLSDRLLGTKARRSWLAAEHLRER
                     GVGTPPPVGYLDRWRGLRLLESYYFSEYVQGLTSFRNELIRLYRHEPECARLMALLET
                     VARAVRALHEAGVVHGDLGNQNILLRRAGAGEWRDVQFVDLNRARIRGALSNRARARD
                     ISRITLPSDFLRVFIEMYFAAPPPPAFLRWERFYRRLFAWHTASRSWRHPLRARRERR
                     AARPPETTYPAEPDIWIWDERSGQAISAMRPPDRRHYYPAGNAWRTAAAVARAAFPVR
                     RQYNRLIAECYARPVDLKRRFGVALHPEEDTVEREAALLEELGPIPVMLRFYRHRGPA
                     HWALVARTARALKERGHPVAAALVQDRASVRDPARWADFADQALGQVDGIADWVEIGH
                     AVNRVKWGVWDWREYGRMAQAVAERAPRYPGLKFMGPAGIDFEYPSVLALLDQAPAGF
                     RFHALSHHLYVDRRGAPENTQAGFDLEKKCALARAIAAASPACEDRLIISEVNWPVQG
                     QGVWSPVGSPHVSPGPRHGDPSVGEQEYADFMIRYLAAAVCSGMAERVYWWRLVARGY
                     GLVDDTDPANWRPRPAYEQLRFFLKQVGGGKFMRRLEAPPGERRLALETEAGRSVVIA
                     WTHGAVREIARPEGCTAALDALGREQAGPGDVVRLGGSPVFLLDAGPAREG"
     gene            28834..29565
                     /locus_tag="PK634_01900"
     CDS             28834..29565
                     /locus_tag="PK634_01900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW96520.1"
                     /translation="MKETSGPVEISVIMPAYNEEECVEGTLRELCGVLRKTGRSFEIL
                     AVDDGSTDGTPAVLKKVAAELPELRVIRLAPNSGQSAATKAGFRAARGGVLVTLDADG
                     QNDPADIPRLLEQLDRCDVCCGYRGKRQDSFAKRAGSRLANAVRNRALKEQVRDTGCT
                     LKAFRAEWARELPLEWRGMHRFLPTLLAMRGARIGEIPVRHRPRAAGRSKYTNWGRLK
                     ETIWDLWAVRWMQKRYRRFAVEEQA"
     gene            complement(29732..29920)
                     /locus_tag="PK634_01905"
     CDS             complement(29732..29920)
                     /locus_tag="PK634_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOW96521.1"
                     /translation="MKATIDKDLCTGCELCVTTAPDVFEMGDDNIAQPKGDSVPAGSE
                     DAAQEAAANCPVECIKVG"
     gene            complement(29944..30951)
                     /locus_tag="PK634_01910"
     CDS             complement(29944..30951)
                     /locus_tag="PK634_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOW96522.1"
                     /translation="MDLSTTYMGMSLKNPLVPSAAPVSESVDKIRQMEDAGAAAVVMF
                     SIFEEQVRQEEQLIDDRLAAGTESFAESLSYFPAVGDYHVGPDAYLELIRKAADATDI
                     PIIGSLNGVSHEGWISYARRIQEAGAKGLELNVYYIPTDPKLTGAAVEQEYLDILKAV
                     KSAVTIPVALKIGPYFSSLANMAHQFDQAGADALVLFNRFYQPDLDLENMTVASTLNL
                     STPEDIRLPLRWIAILHGRIKASLAATTGVHGAREAVKYLLAGADVVMTTAALLKKGL
                     RHLTTLVEDLDNWLEENEYESVRQMKGILSQKTVADPAAFERANYIKILEKYKTEYRM
                     V"
     gene            complement(30970..34539)
                     /gene="nifJ"
                     /locus_tag="PK634_01915"
     CDS             complement(30970..34539)
                     /gene="nifJ"
                     /locus_tag="PK634_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOW96523.1"
                     /translation="MSKNITIIDGNEAAAYVAHKTNEVCAIYPITPSSNMGEWADQWS
                     SEGRTNLWGTVPLVAELQSEGGASGAVHGALQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVSARSLACQALSIFGDHSDVMATRQTGFALLASSSVQEVMDMALIAQAAT
                     LESRVPFVHFFDGFRTSHEVNKVELLDDDQIKAMMDDKLIIAHRARALNPERPVMRGT
                     AQNPDVYFQGRETVNPFYAAAPGIVQKAMDRFAKVAGRAYHLFDYVGAPDAERVLVIM
                     GSGAETALDTVNYLRKNGGDKVGVVIVRLYRPFSAAHLLAALPKTAKAIAVLDRTKEP
                     GSAGEPLYQDVVTALAEALGAGSLAAMPKVVGGRYGLSSKEFTPGMVKAVLDELKKTQ
                     PKNHFTVGITDDVAKTSLDYDPAFSTESDKTVRAMFFGLGADGTVGANKNSIKIIGEE
                     TDMDAQGYFVYDSKKAGSRTVSHLRFGSEPIHAPYLISAANFIACHQAIFLEQVDMLK
                     YAVPGATFLLNSPHGKDEVWNTLPRKVQQQIIDKKLKLYVIDGYKVARDTGMGSRVNT
                     IMQTCFFAISGVLPREEAIAKIKESIKKTYGKKGEEIVRKNYEAVDQTLAHLYEIQVP
                     AQATSAHDMPPVVAAEAPEFVRHVTAEIMAGRGDDLPVSAMPADGTWPTGTTKWEKRN
                     ISLAVPAWEPDLCIQCGNCSLVCPHACVRSKLYDPAQLAKAPASFKSVEWKSKEQPGR
                     RYTVQVTVEDCTGCGLCVDVCPKKDKANPAKRAINMTEKAPILEAERANWKFFDSLPD
                     ADRGALDLGSIKDFQFTLPLFEFSGACAGCGETPYVTILSRLFGERALIANATGCSSI
                     YGGNLPTTPWTVNKEGRGPAWSNSLFEDNAEFGFGFRLTLDKHREYALELLQKLAGDV
                     GADLAGAIAAATQHNEQEIGAQRERVARLKQKLAGVKKPEAAHLLAIADALVKRSVWV
                     VGGDGWAYDIGYGGLDHVLACGRNVNALVLDTEVYSNTGGQCSKSTPRGAVAKFAAGG
                     KPGSKKDLAMMAISYGNVYVARVALGANPRQALKAFIEAEAYEGPSLILAYSHCIAHG
                     IDMINGLKQQKLAVDCGHWPLYRFNPDLAAKGQNPFCLDSKKPSVPLKDYAYNEIRFK
                     MLTRSKPEEAKRLIELGQEDVNERWRIVERMAAAPAGAPVEQL"
     gene            34867..35862
                     /gene="dusB"
                     /locus_tag="PK634_01920"
     CDS             34867..35862
                     /gene="dusB"
                     /locus_tag="PK634_01920"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOW96524.1"
                     /translation="MPLSVTTPWALGGLPLATRAVLAPMAGYTDLPFRRLVRSFGGVG
                     LAFSEMVNPDSILRGRSRKVSALVATAPDDRPLGWQIYGTKPGLLAEAAQWFEARGAE
                     LIDVNMGCPQKKICSRGAGAALMKTPELAVEIARRVVESVKIPVTVKIRLGWNAPDAA
                     AELARGLEAAGAAALTVHGRTRAQRFSGAVDLDGIRQVVEAVRVPVIANGDVFTLAAA
                     REMLERTGAAAIMIGRHTLSEPWFLRDVARDLAGEPPLAAPCFAERLAMMRRLYADHI
                     QLYGERFGVSLFRRWIPRFAKGMGVPRPMMIAFLQAATPAEWEARMAEVERGAGV"
     gene            35859..36161
                     /locus_tag="PK634_01925"
     CDS             35859..36161
                     /locus_tag="PK634_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96525.1"
                     /translation="MKDGMAGRRILLLLVIGLAACARADWPFAPPDPGQSWAQDHVIV
                     RFRTRAVAQRLGRSLDVNPGAAEKALGLPAGAAVAGVASLLRGLEPGLTQQAVTNR"
     gene            complement(36185..36622)
                     /locus_tag="PK634_01930"
     CDS             complement(36185..36622)
                     /locus_tag="PK634_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96526.1"
                     /translation="MGDAGGKWSSAEKMSHFRNLALVALRDGKLAGAERDLLNFLARK
                     WGLSDAQVEEVLAHPDRVHLSIPKDEASRFQQLYDLVDMMIIDGVLGAQEKALCVSFA
                     MALGLNSGLIPVIVEEIVAGDQLLRSEEQIQAVIRARVARPRK"
     gene            36773..36907
                     /gene="rpmH"
                     /locus_tag="PK634_01935"
     CDS             36773..36907
                     /gene="rpmH"
                     /locus_tag="PK634_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOW96527.1"
                     /translation="MKRTYQPSKVKRKRTHGFRSRMATKNGRKVLARRRQVGRKRLVV
                     "
     gene            36930..37319
                     /gene="rnpA"
                     /locus_tag="PK634_01940"
     CDS             36930..37319
                     /gene="rnpA"
                     /locus_tag="PK634_01940"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOW96528.1"
                     /translation="MTDAAGSRRAPDAVLGRERRLIRSALFREAYDQGRRQFGRCMVL
                     WLRSGEGAALRLGVVTGRKVGGAVQRNRARRRLREAFRRNRHRFAGTWDVVLVARRAA
                     LDASWDDLSAELLSLARKAGLMGSEDV"
     gene            37440..37685
                     /gene="yidD"
                     /locus_tag="PK634_01945"
     CDS             37440..37685
                     /gene="yidD"
                     /locus_tag="PK634_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOW96529.1"
                     /translation="MKFLLIMLIRLYRVTISPLLGPCCRFEPSCSVYFIEALQRHGFW
                     RGSMLGARRLLKCHPLHHGGFDPVPASSSLRCPGVDR"
     gene            37682..39460
                     /gene="yidC"
                     /locus_tag="PK634_01950"
     CDS             37682..39460
                     /gene="yidC"
                     /locus_tag="PK634_01950"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOW96530.1"
                     /translation="MKRNDILILVALFALWLAWPALDRHVIKKYFFPAPPPAAVEEAP
                     PADTASEPPALAPAESAPAAEAPAPAAEVVAEAEPEDAGPETTATLEDGLLRLAISSR
                     GASVASAELKQYPLTAEADSAPVLLDFSSARALAYGGLAGLSEAVNFSLVSQAGNEIV
                     LERTAASGLQLQRTFQLEDGYLLKVTDRFSNRGLAALEVPGHVMHTGPMPNLPGESAM
                     KGVVNQGVDTLSPGGEGVQHWGAKLPRWFEEVRKEKGLPRIPERIEWPLDTPMEWVAA
                     KNKYFAQVVVPEGGAEKAVVVARRELPEAERQDPAAKPRGAVLADVAVSVQFGATALA
                     PGEAFTRTVQCYIGPKKFDVLSRHGQHMVDVMEFGFWAAIGKILLQVMNGIHAHLWPH
                     NYGIAIILLTVLIRVVFWPITHKSTESMRKMQEIQPLVTAVRQQYKDNAQKQQQAIMA
                     LYKEHKVNPLGGCLPMLIQIPVFIALFVVLRSAIELRFASFLWIRDLSQPENLLAGKL
                     PIALNLLPIIMAVTMAWQQKLTPSGGDPNQQKMMMFMPIMMLVLFYNFASGLSLYWTT
                     NQCLMIAQQLIAQRRKKAREAAAAAR"
     gene            39476..39973
                     /locus_tag="PK634_01955"
     CDS             39476..39973
                     /locus_tag="PK634_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOW96531.1"
                     /translation="MSDQDTLSQVQDEARQTLGELLRLTGFDAKVEASAPAEQEVLLR
                     IECSDAAQLIGRKGQVLDALQFILGRMLLRKAGRDVHFVVDVGDYRERRKEQVLREAM
                     AAIEEVRKTGRPVKLPVMSAHERRLVHHLAAETPGLETFSEPTGEEGRKRVVISPVGE
                     SAPPA"
     gene            complement(40060..40674)
                     /locus_tag="PK634_01960"
     CDS             complement(40060..40674)
                     /locus_tag="PK634_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96532.1"
                     /translation="MKKWIVLAMVGAMAAPAMAQFAGLPYADGAAAPEAGLVRGSAGA
                     VVGDDANAYGGRVTFGVMEGLALFADLGLLDPDEGDTGFTYQGGGQYTLPLDLPVDVA
                     LRGSIGMASYEVTDDADADWLFVNFGALVSKTIEQFTPYGFLGMCYRDVEIDWEGVGD
                     ASEDETDVAIAGGALFALNEQFSLYGELAHIDDLFFGLGGRFQF"
     gene            40907..41680
                     /locus_tag="PK634_01965"
     CDS             40907..41680
                     /locus_tag="PK634_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96533.1"
                     /translation="MKTGRKHMKLVWLAAWGAGALAPAAGAQTLAQQLLAGYDAVQSV
                     SCEVRRDTETVGGNSARALSRVYFQKPDRLHVENVTPVQRRIVADGETFFSYIEGDPK
                     GFSRPVSQLNEEMTIQLRKIPGTAMDHLLKIGDAPETVLPAAEGFPVRRGYEKGRLFV
                     VLSMDPENRLARIEFFDSPEMKNRTAQYDYSAFQPAAGGAVIPCLHRGVLWSGGVETR
                     ETVRISNLAVNQPVPGSLFVAGPFFEGVDFVDDFGNIYE"
     gene            41755..42051
                     /locus_tag="PK634_01970"
     CDS             41755..42051
                     /locus_tag="PK634_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96534.1"
                     /translation="MKKVIVGMLIAAFVLAGLGTVQAAEKGAGRGGLMGFIAGCCFGI
                     RAGSAYNDGKEIHWREWVRLVPVVSIVVAIWDGIDGANGVTSKDYAKQYGETYY"
     gene            42068..42892
                     /gene="yidD"
                     /locus_tag="PK634_01975"
     CDS             42068..42892
                     /gene="yidD"
                     /locus_tag="PK634_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOW96535.1"
                     /translation="MRTRRIPGGWPGILLFFAAARLGAAGADLAQELFDEGRWAECAR
                     ESRRVLLSDPRNETAALHLAVARLRLGGTDEEPLAALTSARDPEIRARAAYELGRLNW
                     AAGDPAAAYPLLRQAFLETRSQPLSLRSGCSLNELLRAFPNLGKDDLALVQSLETCRP
                     LWSRALRAECAAPRARADRGGWLSAPGKWLVSFYRTQIRPAIGARCSLEPSCSAYFLA
                     ASRAHGLLGLPMMADRLVREPSVVAEARRPVPGDGAPRFADPVSDHDFWFHGGGGN"
     gene            42892..43761
                     /locus_tag="PK634_01980"
     CDS             42892..43761
                     /locus_tag="PK634_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96536.1"
                     /translation="MGARTWSAVVALAWLVPACAAAQDSPGLSLAVELSAEGDHAGAA
                     LEYRRLAGDAPSGEARGGWYWASAFEYWRAGEYELADRMLNRAEDAQPALGAPALLLR
                     GENAQSARKWAESDFYFESVLRGGTADDEKAFASRRLAAGRLRQKDVTGAGAALDLAP
                     VPQEAARAALAEYNRGRDKSPLTGGLLGIVPGFGYFYAGEWASGVRSILLNSLFLFGM
                     VDTADHDQWGAFAVITFFEFTWYSGSIYGGIDGAHRFNRDRLETAADGIRGASAFEPD
                     PAAWPAVRLNFRF"
     gene            43805..44530
                     /locus_tag="PK634_01985"
     CDS             43805..44530
                     /locus_tag="PK634_01985"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOW96537.1"
                     /translation="MRRGLHLCLLLAGLLADPARGAAPEETLRYELVWGLVRVGTAEV
                     TTRLVEEDGRPLLRFRFTAKSNRWVEKIYPVEDCIDSFIEPETGLPVKLVKKTSEGWF
                     RCDDELRFDRERGVAAWVDHVRGTNTEYAVAPGAHDFFSLMRSMRELDLRPGREDRAT
                     IAADDRTHDLVIRVEREETLSLRGARVRAYRLRIASEKEGLFVRKVPGAIWITAEPPR
                     TVAQMQVRVPVGSVRAVLLDESK"
     gene            44527..45372
                     /locus_tag="PK634_01990"
     CDS             44527..45372
                     /locus_tag="PK634_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96538.1"
                     /translation="MKKSLVFMAWALAVGGSLRAGAASSAPAGTGSFSPLARQLWKAG
                     GPGWVRSEKNAFHVYGRSPWEVGDVRAEAESAWPAIEESLGPDPAPRPARIFVVGDRA
                     LWGRVLRGADRRKDSLAIQAGDEVFLLREPDAVADPGRLPHELVHLKLWRRFGDRVPL
                     WLDEGLASYLGWAAAARVARAGRAPLDRRLPLYEAAQLQTMEEVQAVRSYSAMGPAFY
                     RQAEEMVAAVARRAGDGRLGEFAEAVALAGVSWQDYLKQLGLSDVEREDAEKDMRDRV
                     TGKAH"
     gene            45369..46523
                     /gene="holA"
                     /locus_tag="PK634_01995"
     CDS             45369..46523
                     /gene="holA"
                     /locus_tag="PK634_01995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOW96539.1"
                     /translation="MKAGAEKSKRPAKPAGGGAERKAIYLVYGEDEYRVRTRAKELVD
                     SLCPPAEAAFGLEIIDAAVENAAEGILAVRRALEALRTVGFLGGRKTVWLRDATLFTK
                     DIAEDLKRAVEELTDQIKAGLPVGQTLVVSALKVFRGSAFFKACEAAGTVEECAKPDK
                     PWQQAEYARGQVMEEFRRHGLKPVGRALDLFLDRVGMDTRSIVQEAEKLAVYLGGRKD
                     ARPEDVQAIVSLSRESAAWDLADAVGARDLQGALKILRQLVFQGESAIGLLHALEFRF
                     REMIVFREALDRGWARPVGEGYGRKLAWREGPEIDAALKALGKDPREGHWFRGMKLAE
                     QAQRFSAAQLRDGHAILLKAHEAMVSSSVPDALQMELLLLKLLAPSPRDA"
     gene            46776..47771
                     /locus_tag="PK634_02000"
     CDS             46776..47771
                     /locus_tag="PK634_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOW96540.1"
                     /translation="MARPLRLQFPGAIYHVTFRGIERRTIFRDSRDYKRMLEALEEMA
                     DRHRVRVYLVCLMTNHAHLLLETPRGNLSAFMGQLLTSYTVYFNLRHRRVGHLTQGRY
                     KAQVVAGDEYLLKLSRYVHLNPVHIKGAEEWPIEERVRRLRAHPWSTYRSYAGLERAW
                     PWIEYGPIRALIGKGAGGAAYRGFVEECLARSEDDFRAVYRSARLALGSEEFQREVEQ
                     KHIAMGRMRRRKEDVSFRRLLGRCEPKAVLEGVARAFGVKPEDLQKRRRNADFRAAAC
                     WYLHAQGGLTQRDAAMWVNLKSGVAVAYQIRRWQIMSREKRLCSALEKLPADWSR"
     gene            47904..48434
                     /locus_tag="PK634_02005"
     CDS             47904..48434
                     /locus_tag="PK634_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96541.1"
                     /translation="MERAWPWIEYGPIRALIGKGAGGAAYRGFVEECLARSEDDFRAV
                     YRSARLALGSEEFQREVEQKHIAMGRMRRRKEDVSFRRLLGRCEPKAVLEGVARAFGV
                     KPEDLQKRRRNADFRAAACWYLHAQGGLTQRDAAMWVNLKSGVAVAYQIRRWQIMSRE
                     KRLCSALEKLPADWSR"
     gene            48983..49366
                     /locus_tag="PK634_02010"
     CDS             48983..49366
                     /locus_tag="PK634_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOW96542.1"
                     /translation="MKTTEQGYSRRAFLKLAAAGAAGAGVVGWLRFTGSERVVVCSKC
                     AAEFAEGHHYKAGPVRGVYCPNCGVELTRLEYDLRQDAMFEDGDKAQSEEARRKWNYG
                     QVPFPNPKLVSRTDKPAIVLSEIKI"
     gene            49963..51132
                     /locus_tag="PK634_02015"
     CDS             49963..51132
                     /locus_tag="PK634_02015"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HOW96543.1"
                     /translation="MQTANKYLAVENFGLLQRQAGVDPELAELVCACARLFSDSQQPW
                     LFYDAGVSPADLKHDFLCGWLAKKNPQIDPRLLEFLTRYSRLMAARRLPVIFSVGHFA
                     RKHDLSERHVRWISRNVLRFYKEFNIPKSDGGARQILAPEGKLLTLQRWILRHILDKA
                     RPHPVACGFVRGRSIMDNVRPHIGRAVVVRVDLKDFFPSITHARIRKVFQGLGYPYRV
                     AVLLTNLCTVNGRLPQGAPTSPALSNLVFLKADRRFTGLAEKMQFRYSRYADDLMFSS
                     DNPRLACLIPLFKQIVCEEGFTVNEDKVRVMRRGQRQQATGVVLNEKPNVRREHVRRL
                     RAAVHRLRTLGPEDVVLGSRRGGAHDPEYVLRGHLAFLRMVNPAQGAALAERLPA"
     gene            51334..52467
                     /locus_tag="PK634_02020"
     CDS             51334..52467
                     /locus_tag="PK634_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOW96544.1"
                     /translation="MILILQPNVDLEGAEYRHLSDTLALLPNVQTRVHHVRGAQQTLT
                     EIYLIGDTKLLSMEDMRNLPCVESVVRVSQAYRVIGRHKDDHRATSFEYNGVRFGQDN
                     LHVFAGLCAVDNRENVERMMKALRDHGQVCTRMGAYKPRTNPYSFQGHGAHCLPYVFE
                     LAGRYGIRVIAMEITHEDHIDQIRAALDAAGRPTGVMLQIGTRNVQNFELLKHAGRQQ
                     EFPILFKRGFGITLEESLNAAEYLASEGNQRIVFCLRGVKTHMGDPHRNLVDFAHVPV
                     VQRLTRLPVCIDPSHSVGGRGRAPDGLLELFHVTAQGVVAGANLALVDFHPDPDRALV
                     DGPQALHMDELGPFLEDVRIAREAYERRLGRIAAAGGHGDKVR"
     gene            52535..53365
                     /locus_tag="PK634_02025"
     CDS             52535..53365
                     /locus_tag="PK634_02025"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOW96545.1"
                     /translation="MKKRHTAVAMLVLGLGLVVCARAAEPVPELWFPVGEKLTYRIYW
                     GMFKVGITTTATGWIEEDGRTRLAIRFRTRTNRILEKLYPVDDFLEAIIDPVTFLPVR
                     FTKRLKEGRYRADETTVFDHARGVAHWRSNMTDKKKDFAIEPDTRDVISFMYFMRSRP
                     LKIGDTVHARVMADEKLYDLYVNIRRRENLKLPEMGRRPSVKLEPEAAFNGLFVRKGK
                     LWMWVSDDKDRFCTKLAAKVPVASVSLWLTKVEVEPDRVVTGESEDDEELEVDDEPAS
                     "
     gene            53349..54353
                     /locus_tag="PK634_02030"
     CDS             53349..54353
                     /locus_tag="PK634_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOW96546.1"
                     /translation="MSLRPERARELLAAFPRQRILVIGDLMLDRYIYGAVHRISPEAP
                     VPVVSVTHEKSMPGGASNTAWNVRSLGGQAAVGGVLGLDAAGADLKAVLAGGGVGLEG
                     LLEMDGLRTIVKTRIIADRQQVVRVDWDDHAPVAEPRARSFCDLIEREARRAAGIIIE
                     DYGKGVVRQPVVDAVLKVARASGIPVGMDPKEGQELDVRGITLATPNRREAFHLAGVP
                     ETRPQAHPLEDQALLGVSEKLLEKWQAAQILVTLGPQGILLRGRAGELHHVPTRAREV
                     FDVSGAGDTVIATCVLALAAGASPIEAAELANYAAGVVVGKLGTATCSPEELMAQMGK
                     "
     gene            54431..55177
                     /gene="kdsB"
                     /locus_tag="PK634_02035"
     CDS             54431..55177
                     /gene="kdsB"
                     /locus_tag="PK634_02035"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOW96547.1"
                     /translation="MTRILGIIPSRYASVRFPGKSLALIAGKPLVQRVVERVQLAKRL
                     DAVLVATDDERIAAAVRPLGVEAVMTDPALPSGTDRVAAAARGRGADIVINIQGDEPL
                     ADPGVIDRLAGVMASDPKWDMATPACRMEDGPEVDQPAIVKVVCAADGAALYFSRSRI
                     PHIRDAADARGAGPVYWQHIGIYAYRQAALERLVAEPPCLLEKLEKLEQLRALHLGYR
                     IKVIEAAKPGLGVDLPEDVAKAEQRLKEEG"
     gene            55286..56110
                     /gene="kdsA"
                     /locus_tag="PK634_02040"
     CDS             55286..56110
                     /gene="kdsA"
                     /locus_tag="PK634_02040"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOW96548.1"
                     /translation="MAATKTIRVGNVPVGGRAPLVLIAGPCVIESREMCLDLAGRLAR
                     WSKKAGAPLIFKASYDKANRTSHRSFRGPGLTRGLEILAEVKERYGLPLLVDVHHETE
                     VPLAAKVADALQLPAFLCRQTDLVLALGESGRVVNIKKGQFLAPWDLGHVIAKVESTG
                     NRKILLTERGASFGYNTLVADMRSLPVLRETGYPVIFDATHSVQSPGGAGDHTGGDGR
                     FAPALARAAVATGCDGVFLETHVRPEKALSDRENAIPWSALDGLWKQLKAIDDVVD"
     gene            56094..56561
                     /gene="lptC"
                     /locus_tag="PK634_02045"
     CDS             56094..56561
                     /gene="lptC"
                     /locus_tag="PK634_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOW96549.1"
                     /translation="MTWWTERMPGALRLAAAAALLFAAPARAQLVSTGATQTVSGFKV
                     PDYDQNNNLKSVLYGDFARVRPDGIAEITNVKLEFYKEGAVEMTVTSPRCVYNQKDGT
                     ARSDADVEIVREGLKITGKGFWWGAKQERFEIYSKARVEIRGARKGMESGGLP"
     gene            56558..57058
                     /locus_tag="PK634_02050"
     CDS             56558..57058
                     /locus_tag="PK634_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOW96550.1"
                     /translation="MKSMFRWGLGLALAAAARVAAGAEAAAPAPEDPATVITSDRLTF
                     DAKQRIALFEYNVLVTDPKMQLAADKLTVQFDEKGQAKTIKAEGRVTITQLDRTAQSE
                     LATYDVASGKIVLSGKPRITRGRDVLEGDVITYWRNEDKMICQPGARLLIYPEEGGVK
                     DQFRGE"
     gene            57084..>57233
                     /locus_tag="PK634_02055"
     CDS             57084..>57233
                     /locus_tag="PK634_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013746238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW96551.1"
                     /translation="MVEARQIVKAYRGRKVVNGVDLSVNRGEIVGLLGPNGAGKTTTF
                     YMIVGL"
BASE COUNT         9222 a        18475 c        19928 g         9608 t
ORIGIN      
        1 gccagcgctg cgccgccgag ctggtgcccc gcgaggtgcg ccaggccgat ctgccctgtc
       61 cgctggacca gctgcccgcc cggcacggcg aggagatcgc ccgcatcctg cgcgccgaga
      121 ccggcccgct ctccgcccag gaggtcgccg acgccgtctc cgtccagctc tcctacgggc
      181 ccgacgacct gacgctgatc gactggaacg cggcgctgat cttcgaccgc gacgccggcg
      241 acgtgctcga cgtcctcgag ttcgccaaca tcgaactgct cgaactccgc gtgctcgacg
      301 cccagctcga cgattcgctg gaccgttcct acgagctgct ttcgcggttt acctggcagc
      361 gcctgctcgg cctctcccgc atgtccagcc tgcggcgcgt cggccagatg caggtggacg
      421 gggccatcct cttcgagcgc gtcagcaacg cgctgaagct cctgggcgac cagtacctcg
      481 cccgcgtcta ccgcctggcc tcccagcgct tccaccttgc ggagtggaac gccggcaacc
      541 tccgcaagct ggacaccatc gagagcctct acgagaaact gagcgaccgc aacgcgaacc
      601 gccggctgga gatcctcgaa tggatcatca tcgcgctcat cgcgttcgag atcgtgtggc
      661 cgttcgtctc gaagcggtgg gggctgtgaa gaccctcgcg cgccgcctct tctactggct
      721 gccgcccctc gcgtgggcgg gggccatttt ctacttgagc tccctcacgc gcctcccgcg
      781 gccaccggcg tacccgggcg aggacaaggt catccatgcc ctgtttttcg gaatcctggc
      841 cctgtggctc ctgcgcgcgc tggcgggggg gcaggggatg tcggccggcc gggcggccgc
      901 gttcgccttc gccttcgcgt cgctctacgg cgcgatggac gaggtacacc agcacttcgt
      961 gccgccgcgg acgatggatg cggtggactg gctggcggac accttcgggg cggcggtggt
     1021 cttcctggcc gcgcgatgcc tcccgcgcgc ggagggggcg gccggaccat gacgcgcacg
     1081 caggctgaaa aacgggttgc ggaactccgg cgggcactga accgccacaa ccgcctctac
     1141 tatgtcgagg cgcggccgga aattaccgat cacgaatacg acgcgcttta caaggaattg
     1201 cgcgtcatcg aggagcagtt ccccggcctc gtcacgcccg actccccgac ccggcgcgtc
     1261 ggcgacgaac cgctcaagga gttccgcaac gtccggcacc tcgtccccat gatgtcgctg
     1321 gacaatacgt acgatttcga cgagctgcgc gagttcgacg gccgcgcgcg caagctgctt
     1381 cccggcgagc cgatcgagta cgtgctcgaa cccaaggtcg acggcgtgtc catcagcctc
     1441 cgctacgaga acggcgcctt caccctcggc gccacccgcg gcgacggaac caccggcgac
     1501 gacatcaccg ccaacctccg caccatccgc tccctccctc tttctcttgc tcttgctcct
     1561 tctccttctc cttctccttc tccttctcct tctccttctc cccccccgct cctcgaagtc
     1621 cgcggcgagg cctacctgcc cgtcgagggc tttaaaaagc tcaatgccca gcgggagaag
     1681 gacggcgagg agctcttcgc caatccgcgc aacgcctgcg ccggctcgct caagcaactg
     1741 gactcccgca tcgtggcggc gcggcctctt tctgccgtct tctacgccgt cggagcctgc
     1801 gagggcatcg agttcgccga gcaggcggag gtgctcgagg cgctgaaggc gttcgggctg
     1861 ccgaccccgc agttctggcg gcggtgccgc gacatcgagg aggtgatcgc cgccgccggc
     1921 gagttgcaga aactcgaggc gacgctgccg tacgagatcg acggcgcggt ggtgaaggtc
     1981 aacagcctgg cccagtggaa aaaactcggc acgacggcca aggccccgcg cttcgcgatc
     2041 gcctacaagt actcccacga gcaggcccgc acgaagctca aggccatcac cgtgcaggtc
     2101 ggccgcaccg ggaccctcac gcccgtcgcc gaactggagc cggtgttcct cgccggctcg
     2161 acgatctcgc gcgcgaccct ccacaacgag gaggagatcc ggcgcaagga catccgcgtc
     2221 ggcgacacgg tgatcatcga aaaggccggc gaggtcatcc cggccgtcgt cgcggtcgtc
     2281 ccggaggagc gcccgcgcgg cgcgaagccg ttcgacctcg ccaggcacgt ggccgggaag
     2341 tgcccggcct gcggcgggcc gatccggcgc gacccggaat tcgtcgcctg gcgctgcgaa
     2401 aacatcgcgt gcccgatgca gctcaagcgg acgattctgc attacgcctc gcggcgcgcc
     2461 atggacatcg agaacctcgg cgaagtgctg gttaaccagc tcgtggacca gggcctggtg
     2521 aaggacgtgg cggacctgta cggtttagcg gcggagccgc tggcggccct ggagcgcatg
     2581 gcggagaaat ccgccgcgaa cgtggtcgag gcggtcgcgg cgagcaaggg gcgggaattg
     2641 tggcggctga tcaacgggct tggaatcctg cacgttggcg agggcgcggc ccggaagctg
     2701 gccgcacaat tccgcgacct cgacgccctg gccgccgaga gcgtcgaagg cctgcagcag
     2761 gcacgcgacg tcgggcctgt gatggccgag agcgtccatg acttcttccg caacccgcgc
     2821 aaccgcagcg taatcgagaa gctgcggaag gcgggtgtca acttcaagac cgggccggaa
     2881 agcgaagccc ccgccgccgg cggaccgctg gccggcaaga ccgtcgtcgt caccggcacg
     2941 ctcaagaact actcgcggga ggggatcaag gaccgcctgc acgaggccgg cgcgacggtg
     3001 acggacagcg tcagtaaaaa aaccgattac ctcgtcgtcg gcgcggacgc gggctccaag
     3061 ctggacaagg cgaagaagct gggcgtgacg atcctcgacg aggccgcgct cgaaaaaatg
     3121 ctcgccggct gattttccaa acattggaaa acacacccgg gaattttcca gggattggaa
     3181 agcgccgcga cggccggccg ccttcaggat cagcctgcgt tcttctcgcc gcaaaccagg
     3241 aagacgctga acgtgcggac ggcgcctgtc cggccgggcc gtgtgacttc cgcggcggtc
     3301 gttgcggtga tcttggagaa gcccgcctgc ctgaaccagt cgcccagcgc gttcctttca
     3361 aacccgaaat gaaacacgcc ggtattgtcg tggtggaact cgcctccgtc ggaatccagg
     3421 tccgccacgc aaaggcgtcc gggcatcttg agggcggcga acaactggcg gagcagggcc
     3481 gcgggctgtt cgatgtgatg gaaggccatg ctgctgacga tcaggtcgta ggcgccggcc
     3541 aggctgccgc cgggaagcag gtgccttgtg tgaacgttat tcagtccctg ctccctcgcc
     3601 ttggcttcca tcacctcgat catcgcgagg gagctatcgg cgccggtgac cgacttgacc
     3661 aggggggcga agttcagggc gacaaggccc gtgccgcaac cgaaatcaag gatgtccatg
     3721 tcgggcgtaa gcggaacctg tcgcgcgata gcccgggcca cgtccgcggc caatcgtacc
     3781 cgggccggat tttcgtccca gttagccgct tccttatcga agtcgcgtct attggtgttc
     3841 atttggcccc atggttacag gaaaatcagc gcccggttct catcccgggc aggcggctgg
     3901 ggacgcgagg gggcgggctc ctctaattcg tcggggtggg gcggggcggc tggctccgcc
     3961 gcggcgggcg tcgcccgctc ggacaggagc cagtccagtt ccacgcccag tatttccgag
     4021 atggtcagca attcgtagtc caggacgccg cgcaacccgt tttcgatctt ggcgacggcg
     4081 gcccgatcca attgaacgcc ccgggcgatc gcggcatcgg acaattcctg ctgggtgatc
     4141 cagttattgt tccgttttcg agcactcgca atccttctgc cgctgatgtt tttcatgcca
     4201 ttgatcattg tcacacctct atgttctaaa atagatcaat acagaagatg cggcgcgccg
     4261 tcaatgtaat aaggggcgcc gtttattatg ctggagacgg atcgtgatgc gggtccgggc
     4321 gcacggtctg gatatagctc acgccgatga gcgcccgcgt gatgcaggag ttgtcgagca
     4381 ggtggcgcat gtccacgaac tcgccgttct ggacgacgcg ctggatttcg tcctcgaggt
     4441 ctgtcggccg ttcagggaaa ggctgttcct tgaagagcac ggcgtggttg tgcagcaact
     4501 cggtcttggc gccgatattg gccggatgca gataaaagac cttgttcaat tccaccgaat
     4561 cgaaggcatc cagaggcatg gcgagatccg cgccgactcc gcacatgagc atggtcacgt
     4621 tccggtgccg gggctcgcgg tcgcaggcca tccattcgcc gatgttcgag ggatcggtga
     4681 tcaacccacc gttggcgggc gcgagcggcg cgacgggcga gcggagcacg ccggagccgt
     4741 acacggcgac ggcctgcgcc cgcaacgcga gacccagtag gcccttgcag tccggccgcc
     4801 ggcggcgggc cagttgaaaa aggtcccggt acaactgcga gatggtggtg ccggacggct
     4861 cccggctctc gaagaccatg aattcgtcga atccgcccgc gagggagatg ttgtagccgg
     4921 tgcggatcat cacgtgcccc ttgtccgtct tggggatgag aaagtccggc gtgtcgtgtc
     4981 cgtcggtcgg cagccagacc atcgtgccgc cgatcgtcat catttcgccc atgatttcga
     5041 agaaatcctc gggcgcgcgg ccgagtccgc cgagcccgat ggagtattct gtctcgaaaa
     5101 accgcttgga gagcagttgg tccggggtca cgcgggcgtg gaggacgtcg cgtacgtcgc
     5161 cgagcacctc gacggcgccc gggccgccgc cggcgttcat gatacggacc gcgccgtgat
     5221 ccagggcggc ggtttccagg tggtcccagt ggtctcgatt gtgccggcga agggccgcgc
     5281 gccgcgcgct ggccaaggcc ttttcgaggt tgtcgaattg cggcaggacg tcggcaacgc
     5341 ccgtgacttc cagcacttca cggcagtagc cctgtagcct ggccaagatc agggccccgt
     5401 cacgccgcat cagttccttg tggacaggca gcaggatgcg gaggccggcg ctgctgatgt
     5461 aatcgacgcg gctcatgtcc agcagcaggc agaccgtagc ggagtcgaaa tcctgtttca
     5521 gggcgcccgc cagttcgctg gatccgaagg catcgagacg cccatccaat tctaaaagca
     5581 ggatagttcc tttttctctg atggtgattc gcataattta atcatacata taatggcaga
     5641 gggggggggc atgcaaccca tcgacgaact ccctgcggtt cgcttgcccg cttctccccc
     5701 gcagaaggta tcacagttta taaactgtga tacacgattt gtttttcgat attacttaag
     5761 aatattgcac ttcttgcggc caaaatatca cagtttataa actgtgatac atggattcgt
     5821 ccggcgttta ttgcttaaag agtgcggcgg gatcgcaaaa ggcttgcggc atcggaaaga
     5881 cctgtgaaag actgggcgcc gttcgccggg attcggccaa ccaatccggc cgaggcccgc
     5941 gtagtagggc gcgttggccc aacgcgccgc catttcggcg gcttgagcca agccgcgcat
     6001 agcggggtca tgtaaggtca ataggattct gaatgggcgg atggctgaaa ggcgcgctga
     6061 cgggggccgt gttggtcacg gcgctctggc tgctggctcc ccagcgctcg atccagcatg
     6121 aggacggcgc ggtcgagatc accttcatgg gccccgacgg cccgatcaag ggcgcgctcg
     6181 aggactccat ccgcgagttc gagcggttga gccgcctgcg ccacgagcag gatccctcct
     6241 atcccgtcta ccgcgtcatc tcgggccagc acgcctcgcg caaccaggtc gaggacccga
     6301 cccgtttcat cgtcagcgtc gccggaggca tgccgccgga cgtgatcttc ttcgaccggt
     6361 tcgccatcag cgagtgggcc gcccgcggcg cgtttgcgcc gctggacgat cgcctcgccc
     6421 gtgacgaaga ggcttgggcg gcctggcagg ccgatccatc gaagcccgag ccgtggcccg
     6481 gcgcccgcga ggagccgcca cgcgcgaagg gcgcctccgc cctcgcggct atcacgccga
     6541 tccgcgccga cgacttcttt cccgcctgct gggacgaggc cgtctatcgc aacccgctga
     6601 cgggcgaaca gggcaccttc ggcatcccga acaacgcgga caaccgcgtg ctgctctaca
     6661 acaaggatat tctcatccgc cacggcttca ccaacgaact gggcgaggcg caaccgcccc
     6721 gcacgtggga ggaactggag gagatggcga tcgcgatgac cgagcgcgac gcggcgggca
     6781 aaatcaagac catcggcttc atcccgaatt acggtaactc gtggctctat ctctatggct
     6841 ggcaggccgg cggcgagttc atgagcgcgg aaggccggac ctgcacgctg aaccacccgc
     6901 gggtcgtcga ggcgctcgac ttcatgacgc gcatctacga ccggctcggc ggcgcgagcg
     6961 atgtctacgc gttccagacc accttccagg gcgacgcgct cgatcccttc atccagggca
     7021 aggtggccat gaagatcgat ggggtctgga tcttgagctc gctggccttc tacggccgcg
     7081 atctggactt cggctccgcc ccgccgccca tgccgaaacg cgaactggac gccggccgcg
     7141 cgccgatctc ctggctcgcc ggctgggcct acgcgattcc ctcgactgcc cgccagccgg
     7201 acggcgcgtg ggaactgatc cgctacctcg tcagccagcg gtcgctggcc atcatgctcg
     7261 aaagcgaata cttcacgtac gccagccagg gccgccccta cgtgccgcgc cagtacccga
     7321 accgcgcgca gaacgagtac gccttcgagc gctacgtcca gaacaacccg aacatggagc
     7381 cgaagttcaa ggcggccatg aagcagttca acgacctgct gacgaactcg cgctaccggc
     7441 ccgtgacgcc cgtcggccag aagctctgga acgcccagat ctgggcgatg gaggacgcga
     7501 tcttcaagaa gaagacggcg aaggaatcgc tggactacta caccgccatc gtccagcgcg
     7561 acctgaacct gctgctcgat ccgccgaagg gccggccggt ccggagttgg gcctggtttt
     7621 tctgggtgta cggcgcgctg gtcgtgctgg ccgtagtcgc cgcgtggtgg cgggatacgc
     7681 ggcgcaaggg gctggagggc tcgcgcagcg cctacttccg ggccgagtgg cgcggcgggg
     7741 tggcttgcgc gctgccgtat ctcgtcgggc tggttgtctt caccggcggc ccgctgctct
     7801 tctcgatcgt catcagcttc tgccggttcg acgtgttgaa cccggcggtg ttcaccggcc
     7861 tgcgcaacta cctgttcatg ctgcgcggcg acgagctgtt ctggaaatcg ctctacaaca
     7921 ccgtgtacat ggtcctcggc atcccgctgg gcatggcgct cgggctgggg atcgcgctgc
     7981 tgctgaatct caaggtccgc ggcgtggcgg tgtggcggac gttcttctac ctgccgagca
     8041 tcgtgcccgc cgtcgcgtcg agcatcctgt ggatgtggat gttcaacccg cagagcggcc
     8101 tgctgaacgc cgcgctctcg gcggtcggga tcgagggccc gaactggctg caggacgagc
     8161 acacgagcaa gggcgcgctg atcctcatgg gcctgtgggg cgcgggcggc ggcatgatca
     8221 tctggctcgc cggcctgaag ggcatcagcg agagttacta cgaggccgcg gcgatcgacg
     8281 gcgccgggac gtggcacaag ttcgtgtaca tcaccctccc gatgctgacg ccgtacatct
     8341 tcttcaacct cgtcatgggc ctgatcggca cgttccagat cttcacgcag gcgttcatca
     8401 tgaccgcggg cggcccggtg aactcgacgc tcttctacgc ctaccacctc ttcaacaatg
     8461 cgtttcgcta cctgaacatg ggctacgcgg ccgcgatggc atggttcctg ttcctcatcg
     8521 tcgtcgtgct gacggcgatc cagatgaaga tctcgagcaa gtgggtgcat tatgaagggg
     8581 agtgaggaaa aggatgaggg atgaaggatg aatgaatccg cccaatccga cctcattcgc
     8641 cgccggctgg cgggcttgag ccgcgcgctg ctgatctacg gcctgctggt cggcggggcg
     8701 gcggtctttg cgctgccgtt cgtgtggatg atagggacca gcttcaaggt cgaccgcgag
     8761 atattcggcg ggaagatcac gatcttcccc atgcggcccg atcccgtcgc cgcctcgccc
     8821 tacatggacg cgcgctatta ccgtgagccc acggatgccg actacccgcg ggtcaagccg
     8881 gtcatcgagc aggtcctggc ggagtcgggt gccggcgatg cggacattct ggctcccggg
     8941 ttgcacgaac ggctgaagca ggtcttgccc gacgccgcgt ggaaggggac ggacggggaa
     9001 ctcggggcgg cagcgcgggc caggctgacg ccggagctga tcgaggacgt ccgcgacaac
     9061 gtcgcgcggg cgctgctgct gggccccgtc cgcgtccgca gcttcgattt acaggaggcg
     9121 gacctcaccg agggcgcgcc ggtctcggcg ttctggacgg tggagagccc ggtcgtggcg
     9181 cggctggtgg atgagaccaa cgccacgccg ccggcggccg tattgaacta cgattttggc
     9241 gaccagcgcg atgcgtcgat cacgctgacc accacgctgg acctgccgtt cgccgccgac
     9301 cggctgcacc gcgtgacgct gttcctgcgg ccggacgatt cgtggcaccc gctcgacttc
     9361 acgctggaga agggcgggac gctgtaccgc ggaaagcgcc cggaatacct cggcaacttc
     9421 aactggggca gcgtgacgct ccaggaagcc ggcccggacg acgagtccac gaagatccgc
     9481 ttgtggctgc cgtacgagaa agtggatgcc ggctcgcaat acgaatcgcg gccggacaag
     9541 gccaagctga cggtgatcct gcgccggaac acgcaggccg gggcgtggtg ggcgaaggtc
     9601 cagcgcaact accgcggcgc gctggactac ctgccgttct ggcgctacgc ggcgacgagc
     9661 gtattcctcg tcatcctcaa catcgtcggt aacgtcttct cctgctcgct cgtcgcctac
     9721 gccttcgcgc ggctgcggtg gccgggccgc gacctgacct tcggcgtgat gctggcgacg
     9781 atgatgatcc cgccgcaggt gacgatgatc ccgtacttcc tgatcatcaa gtcgctgggc
     9841 tggtacaaca cgctcacgcc gctctggttc gtcagcttct tcggcaacgc gttcaacatc
     9901 ttcctgctgc gccagttcat gaagggcatc ccgcgcgacc tggaggacgc ggcgaagatc
     9961 gacggctgca acgtctggca gatctactgg aacgtcatcc tgccgctgat caagcccacg
    10021 ctcgcgtgca tcgcgatctt tacgttcatg ggcgtgtgga acgacttcat ggggccgctg
    10081 atctacctct ccgaccagcg gctgtacccg ctctccctgg gcctctacgc cttcaacgtc
    10141 caggccggcg gcaactacgg catgatgatg gccggctcgc tgatgatgac cctgcccgtc
    10201 gtcgtcatct tcttcttcgc ccagaagtac ttcatccagg gcgtcacgct gacggggatg
    10261 aagggctgac acgcatagcc gttgtttgca ggcccgtcac cgaacggaag caaagcgtgg
    10321 cgacagcggg ggagttgatg aatccggccg ggtcgggctg gagcactctg tcagaggtcg
    10381 agttcctctt ccaggacatc ggcgagctgc cggccggtct ggaagcccag ttcccggtag
    10441 gcctcgaatt gttgttcgct gaagaactgg tctcccgtgg actcggccgg aaactgcggg
    10501 tgtttccggt ggtaggccag cacgtcttcc ggggtatcct tcatgagggt gggtttgatg
    10561 taaatcagcg cgcctgtgga ctcgtcggcg tagcggatcc ggcctatcac atggcatgcc
    10621 gggtagaacc cgagggcctt cgggatccgc aagggcgcgg tgttgatgtc gatgcgtgtc
    10681 ccgaagtccg cgcgcactag ttccatggct cgaccgaggt cggaaaaccc gcgttccgca
    10741 tccgccgtgg cgtcggacac gatgatgcag cgacactgtc gccggatgag ttcatagagg
    10801 cccaggttct cgaactggcc gccgtcggag aggtggagat agacgctgtc ctcggacagg
    10861 cccttcccgg tcatttcgta tcccatgcat cggtaccaca gcggccgcca gaatgtgttc
    10921 cagcgcggag gggggtgccc ggggcgatgg atccaatagc ccagccggat gttcaggaaa
    10981 gacatgatga aactcaaggc tcgcgagcgt gtggcgtagg tatgaggatc gacggcggcc
    11041 cccgaaacgg ccatggcggt ggccaggttg gtgcgcccga ggccgtactc ggacgtgctg
    11101 gcatatccgg ccgattcgga cccacagtat ttcgccgtga agatgaagtt atcggccccg
    11161 cgttccctca acttgtacct cttggagcct acggtattct gggcggcatt gatgatatga
    11221 tagggcgcgc gggtgggttg aatatccttc aagaggctgt tttccggatc ggcgtccggg
    11281 tactgtgcgc gttccggctc gggcaccaga aggcgggggc gaggcaggta ggcatccctc
    11341 agacgattcc gatagaagcg atgcagggac atgcgattaa tgtccgccag gaggcccagg
    11401 aggagcgcgg cggtgaaaac cgcgggaaat atccagaacg gcatcgccgg ctgaaatact
    11461 cggccagccc acgaaaagag cgtaatggct agcgcataga tgagcaagga aaggcccgcg
    11521 cagaggaaaa atgaccgccc cggggaaagg gcgctgcttt ttgatttgcc gccgatcaag
    11581 gccacgagga tcgaacacac gccgcccgcg gacaggccgg cggggatttt tatcgagcga
    11641 atccaggccg cacctttgca ggaaagcagg tcgtgaatcc aggggacgat gcttacaaga
    11701 agaccgcaga gcccgaacag gagcaagcga cccttccatt tttcgcactg gcattccagg
    11761 cgttgaaacc aaggcgagga ccgggtgagg cccgagagca gggcttgggc gaacatgccc
    11821 gcgaccagca gtgacaggag aatggcgccg ccgattccta tccagatgga ggcgggacgt
    11881 gagcctatgg gcacgcgaag cgcttggatc aacaagaggg ccggagggag caggatcgcc
    11941 aaattcacga gaagccccgt caggaccgcc gccgctacgg cgaacgcgcc catgccgcgc
    12001 cccggcgtaa tgtattgacc atggtctcgc agccagttat ggaccgcccc cgggaattcc
    12061 gcgttgtctg accgccttgc gccaagaggg aattccatgc cgaggcagga cataaaccaa
    12121 gtcagacagc ttcctatata tccgccgccg gagaccgttg aaagaaagtc gattttccaa
    12181 aaatgcccgt atcgttgcaa ggcctggagc aggccaaggt tgaacgtcgc ggatcgtatc
    12241 cccccgcccg agagggctaa gcccacatcc tgtccgggtg tctccggatg ggcgtagggg
    12301 atgcgatgcc tggcgccgga tggatccgcg ttcacctgtt cgcgccgggt cctaatccac
    12361 tcgcgctcgg cggtttttag tctattatcc agaaagcgag gctctttatg ctgatgcgtt
    12421 gtcatgacga tccccctgtt ttcgccgtcg gttgcccatg ccacgcgagc tatttcacat
    12481 cccaccacac tccgatcacg gcggaccggt tcgggccgcc gtaacccagg aactcgtact
    12541 ccgtcttctg ccggttcttt ccgcgtatgg tcgcgacgcg gattccgttg gtccggctga
    12601 agacaaggct gtcgcccgcc gtgttggtgt aaagcgtccc gtcgcgcggc gggtcatcca
    12661 catggaacag cgcgtgaagc ccgtccatgg cccattcgcc gttgacggtc aacacctggt
    12721 ccggatccgg aacgtaccag ccctctacag cccgggtcac ggcctgccgg gtcatgcacg
    12781 aggtcgccag gcccgccagc agcgcgagga cgccgacggc gagggatcgc catagacggt
    12841 tcatggggct cctcctgtag acagtctgcg caccaatttg gcaatcagtg ataaacccca
    12901 gtacgaggtg tgtgcctgga cgaatccctg gtcccgcgcc agcggacggc tgtccacgcc
    12961 gggaaaccgc ttcccgtcga gaggcgcctt caaatggatg cgatccgccg ggtaatacag
    13021 gtcatgccat gggaagggaa actcgccgga cattttcgcg cctttcgtca ggccgaggat
    13081 attcagaggg ctgccgatcg tgaccagcgc ggagacgtcg ccccggcgga gagtcccgtc
    13141 gcgcagggcc tggcagagga tatccgcgac gatgaccgag ccgaggctgt gagcaacaac
    13201 aacgcacccc gggaacatcc tgatcgtgtc gcggaccacc ttgcggattt tccttctctg
    13261 cggcgtgaga cagtagagcg gcacatcggc tagcaggtcg agcaacggcg cggcgagcca
    13321 tgcccccgga aattttactg acaatagacg caggccggcg gcgacccgct tgtcgatccg
    13381 gtcattctca ccctcccaca gggcctcttt ccagaagatg gaacagggcg ggcggcgcgg
    13441 cggtttttct tccccgtact cgatccggcg gaccgggcgt tgcacccggc tgacgtgatc
    13501 cgaaagttcg cgggaaaacc cggcgcgatt ccaattcgtt cccgaaccca ccccatgcac
    13561 ggccacgatg ggatgatgtt gatcgctcat gcgcccaccc cctctccgac gccggcaggc
    13621 tcccctcctc ggcggatcat gatgattcgg agaggcgatg ttgtcaatta acccgtagtc
    13681 ggaaggccat gggccagggg gatggccctc aaggccgacc gttatggcgg gcccgccgtt
    13741 cgcgggccgc gtcttgaagg gcgggggata gttcctgaag aatctgctcg aagtcctccg
    13801 cccccgccgg gtcgccgaga atcacggaga gcaggttgtc cgccgcgcgc cgggtcgcca
    13861 ggtcgtcggt gaaggacggc gaaagccagg ccgccacggt gcggtccggg tcgtagcccg
    13921 ctgcggcgat cgcccgccgg gccgcgcggg acgcggcgac ggcgtaggga aggccggggc
    13981 tctcggcctc gagttccctg gtcgcccggc tgctggcctc ctggaaacgc tccaagtccg
    14041 gcgcggtcag cggcatgacg ggcgccccag ccggcggtat ttcgggtgat aggaccgttt
    14101 tgctcgacgc ggcggcgggc atttcggttg aggtgaccga tttgctcgac gccgcggcgg
    14161 gaggcgccgc ctcccgccgc accaggaacc acgcggcccc agcgagcccg aggagtccga
    14221 ggagcgccaa ggcgggccgg aacgctgtac gaggcccgga catggcgcgt cagggcagga
    14281 agacccgcga ccggaaatgg accggcgtgc cgagggcgtt ggtgacggcg atgctcaagg
    14341 cgccgccgtc ggcctgcccg ggccggttgt aaccgagcgg ggtccacgtc ccttccacga
    14401 ggttgctgca gtatgacaca tcgtacagcc ggctgttggt ggtgggcgcg ccgatctcca
    14461 ccgtaaacgg gcccgcgccg gggccgcccg cgcccgcgac gcccaaccgg gcgaagaacg
    14521 agccgccgtt ggtgggatgc gtgtcggacc agtattcctg caggttgctg aacccgtcgc
    14581 cgtcccggtc gtcgttgtcg cccgagccgg ccccggtcaa ctggtagcgg tcgcgccagt
    14641 cctgcgggat ctggctggtc gcgccctcgc ggacgatctc cacgaccacg ctggtcgagg
    14701 ggccggaccc ggcggcgccg aggcgcgtca ggcggttgaa gaagaagtcg tagtccgagc
    14761 ccgcgcccgc gttgtagttc caggcccgga acacggcgat ggactggtcc gcggcggtga
    14821 ccaggctgcc ggtattcgtg cgcgtgttgc tgccgtacgg cgtgatccgc gcgcggaagg
    14881 tggtcgggct ggtcagcgtg aattcaacat ccagcccggt ggacgtccag ccgacatcgg
    14941 tggtcgtgct gctcatgctg tagaaggtgt cgccgccgtt gaagaagaac tcccacaggg
    15001 tcgtgccggc cgcgttctgc aaggccaccc cggcgccgct cccggtattg atggagccgt
    15061 tgtccatccg caccgacacg gtctggccca ccgccagcgc gttggtcagc aggcgcttgg
    15121 cctcggacag gttgccgctg ttggcgtaca ggccccacgc cggcgcgccg atgttcacgg
    15181 acgcggcgct ggccatgaac cggccgttct cgctcgagtt ggcggaacta gcgtagaact
    15241 gccacgcgcc ccagcccgag ccgccgttgt cggacaggtc ccacgcgtcg ttgtacacgg
    15301 cgttcgtgcc gtcgtccgtc gccagcaccg cgctgcccgc gttcgtggcg gagaggacga
    15361 tccggttggt cccgatcccc agcccgacgc ccgcgaggga ccacgagaac ccgatggcgc
    15421 ccgtgccggt cgcgccggtc aactggttgc tccacgcgag gccggcccag ttgagcgcgt
    15481 tggcgacgcc ctgcacggta aagttggtgg tgccctcggc caccacgatg gcggtcggcg
    15541 gattcgtgac gagcaggtcc ggcggcaggg gctcgttgtc gaggatcgtg accgtctgct
    15601 ggcccggcga gtccaggtcg gccccgacgg ccccgctcaa gccgatcgag aacgtctcgt
    15661 ggccctcgta gctgccgtcg tccgcgagga ccacgtcgac gaacttgttc gccaggtcgc
    15721 cggccgccca gtggagcgtg ccgttggtcg cggtgtagtc cgagcccgcg acggccgtgc
    15781 cgttggccgt ggcgaacgcg acgctgaccg cgccggtggc cgcgccggcg cggctcgcgt
    15841 agacgcgcac gctgcccgcg ttctcggccg ccgagttggt cgcggcggtg agctggatcg
    15901 tgtcgttgac gtcgccgaag ctgacgcgca gcgcggcggc cgcgcccgtg tccggcagct
    15961 tgatgccgat gcggccctcg ccggtgatca gccagccctg cgagcccgcg ccgaagttgg
    16021 cgagttgcgc gagcggcgcg ccgttcagag tcacgccgtg gacgaccgag gttccggggt
    16081 tgtagccgta cacataaaaa tcacgcgccc ccgtgttgta cgtgccctgc ctggcctcga
    16141 tggtgaagtc ccacgcggcg gccgtgcgcg cgctgaccat ccggcgccgg ctgtactcgc
    16201 cgcccaggta gtcccacgtc tcgccgtcgt cctcgtgcag cgtgaactcg ctcgtcccgc
    16261 tgggccagca gtggatgtcc atccacgccg cctggctctg gttggcgtac tgcatggacg
    16321 gacccatcgg gatgatcgcg ccggagcgca cgaagagggg cgccttgccc agcggggcgc
    16381 tgacggtgat cgtctggcca ccggcgtact tgtcgccgga cggcgagctg ttgggcgaac
    16441 cggacggcca gtagtaccac tccacgcccg gcgcgtaggg caggtagacc gtgcgggtgc
    16501 tcgcgccctc gttgtagatc ggcgccgaca ggatgaagtc gccgacgagg aactcgtaat
    16561 cgctcaagcc gtacgtgttg gcgtcggcgg aataatcgaa caccggcggc acgttcatgg
    16621 gctcgccggt ctgggtggac tgataggcca gcgtgtagag gtagggcatg agccggtagc
    16681 gcagcttgat caggtcgcgc agctggctct tgtacggctc gctgaagcgc cacggctcgc
    16741 ggtcggccga gttgtcgttg gcgtggttgc ggaagaacgg ggtgaggcag ttgaactcgg
    16801 tcgagcgcac catcagctcg gccgagggcg tgccggagaa gccgcccacg tcgttgccgt
    16861 accaggccgc gccggagatc atcgcgccca tgccgaaccg gatggtcgcc cgctggtaga
    16921 accagttggc cgccgtgtcg ccgctccagc tcaccccgta gcgctgcagg ccggcgttgc
    16981 ccgagcggct caacacgaac ggccgcttgt tggaatcctt cgccagcatc gtgttgtagc
    17041 tctgccgggc gcagcgcagg ccgaagtagt tgcgctcgtt ggaccaccag cgccgggagt
    17101 cggtgctgga cgtcccgaac ttcccgtcca cccacagcag gccgttgtac gggatcccgt
    17161 cgccgccctc gggctcggtc aggtcgttcc agatgccgga aaacgcgatc gagttgaacc
    17221 agctcacgat gcgatccttc caccacgtgt tcatcggcgt gctgctgtag tcgaaccacg
    17281 agaccgcgcc ggtgtagatg ttgcgggtga cggtgctccc gccgttgtcc ttgatgaaat
    17341 gcatgttgtt gttggcgtcc gtgtagtggg ccgtgtcgcc gggctggagc cacggctcga
    17401 tcagcggaac caccttcacg ccccgcgcgt tgcagtagtt gaccatgccc gccgggtcgg
    17461 cgtagcggga gttgaacgcg agctgacgga tctggccgtc cccgatgttg ccgtccgcgt
    17521 tcgcgtccat gtagtcgagg tcgaggtaca cggcgtcgag cgggatgtcc tccaccgtgg
    17581 cctggttggc gatgtactcc acccacgcct ggttgtcgta cgagaagcgc gaaaggtggt
    17641 gcccgaggcc ccacttcggc agcatggtcg ggcggccggt cagctcggaa aaccggttga
    17701 tgaccgacgg catggtgtgc gaggccccgc cgccgatgaa gaagtaatcc atgcgcccgt
    17761 cgccggcctc gaacgagtag cgggtttccg aagagacgtt gaacttgaac agcggccggc
    17821 agggattgtt gaagaacacg ccgtacacga aggccgggtt gccgccggcc gccggctgca
    17881 caccgtagaa gaacgggatg ttcatgtagg tcgggttctg gtactcgccc cagttaaagg
    17941 tccccgtgtt ccagcagtcg atctcccgcc cgcgccggtt catcgggccg ccgtactcgc
    18001 ccagaccgaa gtaggcctgc ccgcccggca tgatcttctg gcatttcagc tttgaggagc
    18061 ccgtgccgcg ctggcccgtg tagttgtagc ccgcgtcgta ttccatgaag tcgtcctgca
    18121 gcagatagaa cccgctcttg tccttgaagt gaatcttgac cggcgtcttc tccacctcga
    18181 tgtccagttg gctggtctgg atcacgtact tcgagccctg gtcgctgatc gtcgcgcccg
    18241 cggacgtcca gtcggccagc ggcttggcga tcatgggctg gtccgtctcg aacgggccga
    18301 cccagtggta atccacgcgg accacgtccg ccgcgaacgg ggtcacctcg accgagccgc
    18361 cgccgctcaa cgcgaacgag gcggtgatgt tccccgacgg gctgttcgtg gtgagcgtca
    18421 gcccggttac gttgcccgcc gtggttgccg cggctgaaat agagaacggc gcaagaagca
    18481 gcgacgcggc ggccagggac agcaggacgc gacgccggga gaaggcctca acgcggatgt
    18541 ccatgctcct gttcttgctg ctcatgcttt acctcctccg cgtatcggca tcagatcgga
    18601 tcacctcgtc cataataacc agccggccgc gtttagtaaa gcgttttaac gcgttttggt
    18661 ggccaaggca tgaggccggc gcaaagaggg ctgggccagg cttggctgtg ttgtccggct
    18721 tcttctgcgg gcgtgacgcg gacggcgaag aaacggtagc atggccgcgt tgcagtcccg
    18781 gctcgcggaa ggaggcgtgc catggaaaaa cagaaacccg cccaaaaagt cgcctttatt
    18841 ttgatctgcc tcggtttgat gatgcgggcc tccgcggcgg ttacgccgac gctcaccctc
    18901 tggtccagcg agcggcggac ggtgaacacg gccgtggacc ggatagatac caacaggctg
    18961 tacgtggcca cgcgcgcggt cgaggtaagc gggaacgtgc aggatcccgg tggaggcgag
    19021 gccgtcgttg tggaggacct gctggtccgt atggtgctgc cggccggcgt ggccttgagc
    19081 gaggcggatg atctggaggc tgcgccgggg atgtacgccc tgtgggagcc cgcccagacg
    19141 gcgctcgtga cgcggacggc ggggcctttg accaacgggg tgagtttccg gatcaccgcc
    19201 cttctcgtcg attccaacgc gtggctgttg cccccggtgg acgtatcgcg cgcgtgcgat
    19261 gtcgcgcgga tcggggcctt gggagtgatc acccagtcct tcgtggtgac gctggggatt
    19321 gcgccgggat cgctggccgg atgcgagtcg gtcgaggtct cgctgccgga ttggccctat
    19381 cagcaagccc cggtccaggt gagcctggcc ggaagcgcct ggccctccgc cttcgagcag
    19441 gacctctggg gcgcgctgcc ttcctatcac gcgacaaacc cggcctcgct gggcgggttc
    19501 tacactttca ccaatgtgct gaccgtctcg aacacgacga ccgagacggt agatttcatg
    19561 ccgcaggccg aggcgcgctg cgtcgagcgg gtggcgtccc tgggggcggc gcgacggggc
    19621 tttgtacaac tgggcgtcag caacgggccc atctcggtga tctcggcccc gcacgcggtg
    19681 atctgggacg gccaatggcg gcagggcgag acggtcctga cgctggcggc gaagtggtcc
    19741 acggatgttc cggcgctgac cgacatcaag tgcgtccggg cggctacgat cgcggtgaac
    19801 ggcctggata tgcaggaact catgttcagc gcggaaggcg ccaacctgat gggcggcctc
    19861 ctggtcgcgc cgggcggcga aacatgggac atggagccgg aggggggcga ggggctttac
    19921 ttcgagcccg gctcgcgcca tgcggcggac ctggcgggct tcaccaatgg atcgtaccgg
    19981 attgacctgc tgggcttcac caacggcgtg gcggtgtcct acgccttcga cctgcgcggc
    20041 gggtccgtga cgcaggcccc cgcgttccgg tcgcccggac cgttcacgaa ccagccccgg
    20101 ccgctggtga gctggcccgg ggcgacgccg accaacgtca gcgccgtcct gctgatcctg
    20161 aacaacgcct ggcaggattc ggaggagttt caaatgtggc cgggcgccgg ccagacgaac
    20221 catgttcccg agaacgacct gtttgccgcc atcccgtgct cgctcatgta cgccgacgtg
    20281 ctctccggca gttccggcgg ggccagcctg ctgtcgggat atgtttccca acgccggggc
    20341 ctgttcaacg tgctcaccaa cgaggcgatc ccggccgcgg tgcacctggc ggccgagccg
    20401 gcggcgacgg tcgtggacca accggttgcg ctgggggttg tgaacggcgg cttgttcccg
    20461 ggcgcccacg tcgccttcca gctgttcttc ggcgatggcg ggcagacgaa cgctccttat
    20521 gcgcaacgga gctatgcggc ggccgggatc tacaccgctc gcgtcgtggc cgccgacgag
    20581 ctcgggatcg ccgcgacggg attcgtggcg gtggccgtgt atccgtcccc ggcgttcgat
    20641 acggcgacgc ggacgggggc gctgctcgac ctctcgctgt tcgccgtgag cggtgccgtg
    20701 taccgcgcat attgcgccga cagcctgcgc ccccaggact ggatgccgat ggccatggtg
    20761 ctctccggga cgggcgggcc catgcgcgtg cacaccgctc tctcgactgc gcggcgattc
    20821 taccggctgg aaagttccat gcccgaaagc ggcgggaatg tcggctttga accgctggag
    20881 tttccggtcc gccgcaccgg cgatatcatc cgggtggcgg ctttcggcat cccgaactgg
    20941 agcggaaccg acccgcacaa cgggatcgat ctggaagtga accatggcct ggcttcctcg
    21001 gagatcgttt ccccggtgac gggtgtcgtg gagcgcatcg aggcctcgga gaccccgttt
    21061 tcggagccgc ccggccaact catggtcacg gtgaccatcc gcacgccgct cgggaccgat
    21121 gtgagcctgg tgctcgaacc ctccacggcc gatcccgtcg tgaagacgaa tcagttaaac
    21181 gcggtgcatg tgagcgaggg gcagggcgtg ggggtcggaa cgcccgtggc ggacctgatc
    21241 gtaggcgcgc tggggtatcc gcacctgcac tacatggtca tgagaggcga cgagtttgtc
    21301 tgcccctatg gatattcctc gcccgcggcc caggcgatct tcgagtcgct ggccggcctc
    21361 ccgggcagca gcctcccgga cgggaatatc tgttacgggg agccttgaga ggtcccaccg
    21421 cgccccaagc cgacgttgac gaccggcccc tccggatggc accatggccc tgaactgcgg
    21481 ggccgcggaa aggggatcaa ggcatgccgg atcgccggat ggaagggctg gagaactacc
    21541 tgctgacggt catcgaggac cgccggccgg gccgttttgc cgccctgctc cggttcgggc
    21601 tcaagcagct ttcgctcgtg ttcgcgacca tcgtccaggt ccgcctctgg ctgtacgcgc
    21661 gcgggctgtt ccgccatcac acgctgggct gccaggtcat cagcgtcggc aacctgaccg
    21721 tcggcggcac cggcaaaacc ccggtggtcg agatcttcgc ccgctcgctc cagcgccagg
    21781 gccgcaacgt cgccatcttg agccgcggct acaagcgcga caagccgccc ttccttaccc
    21841 gcctggcccg ccgcctctct ttccgcaagg acgccgatcc cccgctggtc gtctccgatg
    21901 gacagcggct gctcctcgac tcgaccaccg gcggcgacga gccctacctg ctcgcctcga
    21961 acctcccgaa cgtgaaggtg ctggtggaca aggaccgcgt gaaggccggc cgctacgcga
    22021 tcgagaagct gggctgcgac acgctcctgc tggacgacgg cttccagtac ctgcgcctca
    22081 aacactgggc ggacatcgtg ctcgtggacc gcaccaaccc gttcggcaac aggcggctgc
    22141 tgccccgcgg gatcctgcgc gagccgatcc gcaacatccg gcgcgcctct ttcatcttca
    22201 tcacgaagtc catcggcgac ggcgcgccgg acctgcgaaa ggcgctccgc gaactcaacc
    22261 ccaaggcgga aatcatcgag tgccggcact cgcccaagta cctgcgcgac gtattcacgc
    22321 gggagaaaaa aggcctggag ttcctgcgcg acctggagat cggggcggtc tcggggatcg
    22381 cggcgccgaa gggcttcgag gatgagctgg tccggctcgg cgccaaggtc atctaccaca
    22441 agcgctacgc cgaccaccac cgctataccc agcaggaact cctggacgcg atcaaccgca
    22501 gcctgcaacg cggcgcccgg gccatcgtga cgacggagaa ggacgccgtc cgcttcccca
    22561 tgatcgagcg gcgcgacctg ccgatctact tcctgcgggt ggagatcgag ctgttgagcg
    22621 gcgaagagga tttccatgcc tgcatctccc gcatctgctt caagtgatcc cggcgcgacg
    22681 ctcgtctgcg cccccggctg ggtcggcgac gggatcatgg ccatgcccgc gctccaggaa
    22741 ctccggcgga ggaatcccga ttcgcggatc gtgctgctgg tcaaaccggc gatgcgcccg
    22801 ctctgggaat tgcaccgcgc cgcggacgct ctgctggagt ggccgtccgg cttgcggccg
    22861 gctgtaacgg ccgtctccgc ggccggttgc gagcgggcgg tcatcttgcc caattccttc
    22921 cgctcggccc tcgttccctg cctggccggc attcccgagc ggcgcgggcg gccgggccac
    22981 tggcggcgct ggctgctgac cgatgtcgtg gagccgagcg gcgatccggc gcgcgcgcac
    23041 caggtatacg agtacctcga cgcgctcgtc cccggcgtcc cgacgggcgg gttcgaggcg
    23101 ccgcgcctgg ccgtgccacc ggcggcgcgc gagaaggccc gggagcggct cgcgggcctg
    23161 ccggccccga gggtggcgct gctgcccggc gcggcgcggg gcccgtcgaa gcagtggccg
    23221 gccgggcact tcatcgaact gggccgccgg ctggccgaac ggcccggcgc gggcctggtc
    23281 gtgctcggca gcgcccggga gacggaactg tgcggccgcg tcgcctcggg catcggggcg
    23341 gcggcgcgca gtctggccgg ggaaaccagc ctggcggact gggccgcgct gctggaggcg
    23401 tgcgacctcg tggtctgcaa cgacagcggc ggcgcccatg tcgcgtcggc cgtcggccgg
    23461 ccggaagtcg tgatcttcgg gatgaccgat ccgagccgca ccgcgccgct cggcgaccgg
    23521 cgccggatca tccgggcgga aggcccatgt tcgcgcgaca tcgcccgcca cgatcccgag
    23581 gcgataaaaa aactggcctc gatcacgccg gaacaggtat accaagccgc gctggagtgt
    23641 tgggcgggct aggctccgcg ggcccggggc tttcccccgg ccggcgggcc atgcgttata
    23701 atccgccaag ggataaccgg atgcgcgaac agatctccgc ctgcataacg gcctgcgacg
    23761 aggagcagaa catccgtcgc tgcctcgaaa gcgtgaagtg gtgcgacgag atcgtcgtcg
    23821 tggacagctt cagcaaggat cggacgatcg acatctgccg cgagtacacg gaccgcgtgg
    23881 tgcagcacga gtggctgggc tacatcgggc agaaaaacct gatccgcggc atggcccggc
    23941 atccctgggt gctgttcgtg gacgccgacg aggaggtctc cccggcgttg cgcgatgaaa
    24001 tccagtcgct gttcggggca ggcgccggcg ccttcgcggg ctttcagttc ccccgcatgg
    24061 tctactacct cggccgctgg atccggcacg gggagtggta cccggacatc aagctgcgcc
    24121 tgttccggaa ggacctcggg cgcagcgaag gccaggagcc ccacgaccgc gtcgcggtga
    24181 acggcccggt gaagacgctg aaggcgcccc tccatcatta cacgtacaac gacatctggg
    24241 aacacctgga gacgatgaac cgcttttcaa cgatcaccgc gcaggagaaa tatcgactgg
    24301 gcgagcgctt ccgctggcgc gaattcctgt tccggccggg ctggcgcttc attaaggcct
    24361 actttctcaa gggcggcctg ctggacggcc cgccgggcct ggtgatcgcc ctgatcagca
    24421 tgttcggcgt gtacatgaag tacgccaagt tgtgggaact ggaacaccgg aagggcgggg
    24481 cggcatgacc gcgccgcgcg ccacgcgcat ccgggcgacg ctggcggtgc tggcttacct
    24541 ggccggcacg ttcctcgcgg ccgccgtgac cgccgtcgcc cttgtccggc tgatgccgca
    24601 cgcgccggag gaaatggccc gcgcggtcct ccgcaagggg ccggacaagg tcatgagccg
    24661 gtgcctgcag gtgtttctcg tcctcctcct gccctggctt ctccggcgcc tgggctggcg
    24721 cggctggcat gacctcggct attccgcgga ggatcggccg gcggcgcggc ccgggctcag
    24781 ccagctcggg cgcggcgcgc tcttcggcct gctgacgatg ggggcggtgg ccctggcgat
    24841 gtccgccgcc ggcgcccggg tgtacgtggc cgatcacggc tggaacctga atccgctgat
    24901 gctggttgtg tatgccggcg cggcgctggc ggtcggcttt ttcgaggaga cgctggcccg
    24961 cggcatcctg tatcgggtgc ccgcccggat ctggggcgcg ctgcccgccg ccctggtcgg
    25021 cagcgtcctg ttcgggctgg cccacttcgt ggagaccgat cccgccgcgt tcgagcgggc
    25081 cggcttctgg ccggccgtcg ccgcggtcct gaaatccacg cgggccttgc cggccttctc
    25141 cgtggcggag ggcgtccgcc tcgcgaacct ggccctcctg ggcggcgtgc tgtgcgcctt
    25201 cgtggcccgg accggcacga tctggttcgc gatcggcgcg cacagcggct gggtgttcac
    25261 gatccggctg aacggcctgc tcacgacgcg cgtgccggaa tccgggggcg gctggtggaa
    25321 cggcttgcgc gccgacggca cggattccgc gtggacgatg ctgatcatgg cctcgatgct
    25381 ggcggccgcg gcctggtggc cgcggcgaag agcgcgcgct tccgcggcgg cgggcacggt
    25441 ccggcgcgac ctgccctggg aacggtgggc ctgggccctg ctgctggcgg ccgcgttcgg
    25501 cgtgcctttt tccatcgcgc tggcccaggt ctgcggcggg ctgggcctgg cttgcgcggg
    25561 cctggccgcg atccggggcc ggctgcgcat cgcctggccc ctgttgttct ggccggcgct
    25621 ggccttcgcc gcgatcgccg tcctgtccgt cgtcctcggg cccgagccgt tccggctggt
    25681 gaagaagacg ggaaggctcc tctggttcct tctcatcccg gtcacggccg ccctggcgcc
    25741 gaccgtgccc cggcgcacgg cgctgctgaa agcgttcgcc gccggcagcg gcgtgctggg
    25801 cctgaagatc gtcctgctga actcgtggca ggcctggcgg gcgcagacgg tggtcctggc
    25861 cggcatcccg gactacttcg gccggatggt cgcgctgggg tccatgaccg ccgggcaaat
    25921 gctgatgatc gggctcctgg ccacggcggc gctgttcctg tggcggcgcc gccgcggggg
    25981 catccccgcg ggctggtggg cgctggcggc ggcgcaggcc gccggcctgg tgctcaactt
    26041 caagcggggc tcctggatca ccgccgtcgg gctcggggcg gtgagcctgg cggcccggcg
    26101 ccgctggctc tggttgcttg gcctgctcgc gctggtcggc gcggccctgt gtctgcatcc
    26161 cgttcgcggc cgcgtggagc agctccaggt cgagtgggat acccagggcg gaggccggct
    26221 caccatgtgg acccggatcg cgcccgcgct ggtggagcag tatccgctgg gcgtaggcta
    26281 ccgctcggtc acggcgcccc tgctcatgcg gatcgatccc ggcgtggagc ggaagcggaa
    26341 ccacctgcac tcgaaccccg tccaggtgct cgtcgaaacg ggctggatcg ggctggcggt
    26401 gtatctggcc tggatgggcc tgggcctggc cgaggccgcc cggcggctgc gcgccgcgcc
    26461 gatggacggc gaacgccgcg cccaggccgt cgccttcacc tggatgctcg cggggctcct
    26521 gctcaacggg ctggtggagt acaatttcgg cgatacggag ctgatgatcg tgctggctat
    26581 cgtcctgggc gcgctgtcgg ccgatcccgc ggaggacccg gcatgaagct gtcgcctgcg
    26641 ccccgcgtgc gtccgagccg gtacagcggc catctcgatc ccgacttctg ggagccgggc
    26701 ctgccggaga agatggagtc gctgcccgcg ctgcttgcgg aaggggagac cgtccaggtc
    26761 ggcgggcgca gtcgcgtggt gcggctcgtg ctgttccgga agcgccggcc ggtcgaggcg
    26821 gcggtgaaat ccttcgggcg cgcctcgctg ctgaagaact tgtccgaccg gttgctgggc
    26881 accaaggcgc gccgatcctg gctcgccgcg gagcacctgc gggagcgggg cgtcggcaca
    26941 ccgccgcccg tggggtacct cgaccggtgg cgcggccttc gtctgctgga atcctattac
    27001 ttttccgagt atgtccaggg cctcacgagc ttccgcaacg agctcatccg cctctaccgc
    27061 cacgagcccg aatgcgcccg gctgatggcg ctgctcgaga ccgtcgcccg cgccgtgcgg
    27121 gcgctgcacg aggccggcgt ggtccacggc gatcttggca accagaacat cctgctgcgc
    27181 cgcgcggggg cgggcgaatg gcgcgacgtc cagttcgtgg acctgaaccg cgcccggatc
    27241 cgcggcgcgc tctcgaaccg cgcccgggcc cgggacatct cccgcatcac gctgccctcc
    27301 gatttcctgc gcgtgttcat cgagatgtac ttcgccgcgc cgccgccgcc ggccttcctc
    27361 cgctgggagc gcttctaccg gcgcctcttt gcctggcaca cggcgagccg gagctggcgt
    27421 cacccgctgc gcgcccgccg ggagcggagg gcggcccgtc ccccggaaac cacctatcca
    27481 gccgagccgg acatctggat ctgggacgag cggtccggcc aggcgatcag cgccatgcgg
    27541 cctcccgacc gccgccatta ctacccggcc ggcaacgcct ggcgcacggc ggcggcggtg
    27601 gcccgcgccg cgtttccggt ccggcgccag tacaaccgtt tgatcgcgga atgctacgcc
    27661 cggccggtgg atctgaagcg ccggttcggc gtcgcgctgc atcccgaaga agacaccgtc
    27721 gaacgcgagg cggcgcttct cgaggaactc gggcccatcc cggtgatgct ccgcttttat
    27781 cgccatcgcg gcccggcgca ctgggcgctg gtggcgcgaa cggcgcgggc gctgaaggag
    27841 cgcggccatc cggtcgcggc ggcgctggtc caggaccgcg cctcggtgcg cgatccggcc
    27901 cggtgggcgg attttgcgga ccaggcgctc gggcaggtgg acggcatcgc ggactgggtg
    27961 gagatcgggc acgcggtcaa ccgggtcaag tggggggtgt gggactggcg ggagtacggc
    28021 cgcatggcgc aggccgtggc ggaacgggcc ccgcgttacc cgggcttgaa gttcatgggg
    28081 cccgcgggga tcgatttcga gtacccgtcg gtgctggcgt tgctggacca ggcgccggcc
    28141 ggcttccggt tccatgcgct gtcgcatcac ctgtacgtgg accggcgcgg ggcgccggag
    28201 aacacgcagg cggggttcga cctggaaaag aaatgcgccc tggcccgcgc catcgccgcg
    28261 gcgtccccgg cctgcgagga ccggctgatc atctccgaag tcaactggcc ggtgcagggg
    28321 cagggggtct ggtcccccgt ggggtcgccg cacgtttcgc cggggccgcg gcacggggat
    28381 cccagcgtcg gggagcagga gtacgccgac ttcatgatcc gctatctggc ggcggcggtc
    28441 tgctcgggca tggcggagcg cgtgtactgg tggcggctcg tggcccgggg ttatgggctg
    28501 gtggacgata ccgatcccgc gaactggcgt ccgcgtccgg cctatgagca actgcggttc
    28561 ttcctgaagc aggtgggcgg cggcaagttc atgcgccggc tggaggcgcc gccgggtgaa
    28621 cgccgcctgg ccctcgagac ggaggccggc cgctccgtcg tcatcgcctg gacccacggc
    28681 gccgttcgcg agatcgcccg gccggaaggg tgcacggccg cgctggacgc gttggggcgc
    28741 gagcaggcgg gcccgggaga tgtcgtgcgc ctgggcgggt ctcccgtttt tctgctggat
    28801 gccggcccgg cgcgcgaagg ataattcgct gcgatgaaag aaacctccgg tcccgtcgag
    28861 atatccgtga tcatgccggc gtacaacgag gaggagtgcg tcgagggtac gctgcgcgag
    28921 ctgtgcgggg tgcttcggaa aacgggccgg agtttcgaga tcctagctgt cgacgacggg
    28981 tccacggacg gcacgccggc cgtgctcaag aaggtggccg cggagctgcc cgagttgcgc
    29041 gtgatccggc tggcgccgaa ctccggccag agcgcggcga cgaaggccgg gtttcgcgcc
    29101 gcgcggggcg gcgtgctcgt cacgctggac gcggacgggc agaacgatcc ggccgacatc
    29161 ccgcggctgc tggaacagct cgatcgctgc gatgtctgct gcggctaccg cgggaagcgg
    29221 caggattcct tcgccaagcg ggcgggcagc cggctggcca atgccgtccg caaccgcgcg
    29281 ctgaaggagc aggtccgcga tacgggctgc accctgaagg ccttccgcgc ggaatgggcg
    29341 cgggaactgc cgctcgagtg gcggggcatg caccggttcc tgccgacgct gctggcgatg
    29401 cggggggcgc ggatcgggga aatccccgtg cggcaccggc cgcgggccgc gggccggagc
    29461 aagtacacca actggggccg gctgaaggaa acaatctggg atctctgggc cgtgcgctgg
    29521 atgcaaaagc gctatcggcg atttgccgtc gaggaacagg cctgacccgt cccgtttctc
    29581 cggatacgag ggcctgggcc ggccgggggc gatgctgttc cggcgcgcgg ccgatttcgg
    29641 ttgttccccg gaatcagccc cgcgaattga agcgtaaaaa aaacgagggg ccgaaaggcc
    29701 ccccgtttgc gtcagcgcga tggcggatcg cttagccgac cttgatgcac tcgaccgggc
    29761 agttggcggc cgcttcctgg gcggcgtcct cggagccggc gggcacgctg tcgcccttgg
    29821 gctgggcgat gttgtcgtcg cccatctcga agacgtccgg ggcggtggtc acgcacagct
    29881 cgcagcccgt gcagaggtcc ttgtcgatcg tggctttcat ggtgttctcc tttggtggcc
    29941 ggatcaaacc atccggtact cggttttgta cttctccaga atcttgatgt agttcgcgcg
    30001 ctcgaacgcc gccgggtcgg cgacggtctt ctggctcaag atgcccttca tctgccggac
    30061 ggactcgtac tcgttttcct cgagccagtt gtccaggtcc tcgaccagcg tggtcaggtg
    30121 gcggaggccc ttcttgagca gcgcggcggt cgtcatcacc acgtccgcgc cggcgagcag
    30181 gtacttgacc gcctcgcgcg cgccgtgcac gcccgtggtc gccgccagcg aggccttgat
    30241 ccggccgtgc aggatcgcga tccaccgcag cggcagccgg atgtcctccg gcgtgctcaa
    30301 gttcaacgtc gaggcgaccg tcatgttctc caggtcgagg tccggctggt agaaccggtt
    30361 gaagagcacc agcgcgtccg cgccggcctg gtcgaactgg tgcgccatgt tggccagcga
    30421 gctgaagtac ggcccgatct tcagcgcgac cgggatcgtg acggcggatt tgaccgcctt
    30481 caggatgtcc aggtactcct gctcgacggc ggcccccgtc agcttcgggt ccgtcgggat
    30541 gtagtacacg ttcagctcca gccccttggc gcccgcctcc tggatcctcc gcgcgtacga
    30601 gatccagccc tcgtgcgaca cgccgttcag gctgccgatg atcgggatgt ccgtcgcgtc
    30661 ggcggccttg cggatcagct ccaggtaggc gtccgggccg acgtggtagt cgccgaccgc
    30721 agggaagtag gagagcgact cggcgaagct ctcggtcccg gcggcgagcc ggtcgtcgat
    30781 caactgctcc tcctgccgga cctgctcctc gaagatggag aacatgacca cggcggcggc
    30841 gccggcgtcc tccatctgcc ggatcttgtc cacgctctcg gagaccgggg cggcggacgg
    30901 cacgagcggg ttcttcaggc tcatgcccat gtaggtggtg ctcaagtcca tggcgtgctc
    30961 cttttcttct tacagttgct ccacgggcgc cccggccggc gcggcggcca tccgctcgac
    31021 gatgcgccag cgctcgttga cgtcctcctg gcccagctcg atcagccgct tggcctcctc
    31081 gggcttgctg cgggtcagca tcttgaaccg gatctcgttg taggcgtaat ccttcagcgg
    31141 gacgctcggc ttcttcgagt ccaggcagaa cggattctgg cccttcgcgg cgaggtccgg
    31201 gttgaaccgg tacagcggcc agtggccgca gtcgacggcg agcttctgct gcttgaggcc
    31261 gttgatcatg tcgatgccgt gcgcgatgca gtggctgtag gccagaatca gcgacggccc
    31321 ctcgtacgcc tcggcttcga tgaaggcctt cagggcttgc cgcgggttcg cgccgagcgc
    31381 gacgcgcgcg acgtacacgt tgccgtagct gatggccatc atggccaggt ccttcttcga
    31441 cccgggtttg ccgccggcgg cgaatttcgc caccgcgccg cgcggggtgg acttggagca
    31501 ctggccgccc gtgttggagt agacctccgt gtcgagcact agcgcgttga cgttccggcc
    31561 gcaggcgagg acgtggtcca ggccgccgta cccgatgtcg taggcccagc cgtcgccgcc
    31621 caccacccag acgctgcgct tgaccagcgc gtcggcgatg gcgagcaggt gcgcggcctc
    31681 gggcttcttc acgccggcga gcttctgctt cagccgggcg acccgctcgc gctgcgcgcc
    31741 gatctcctgc tcgttgtgct gcgtcgcggc cgcgatcgcg cccgccaggt ccgcgccgac
    31801 atcgcccgcg agcttctgga gcagctccag ggcgtactcg cggtgcttgt cgagcgtcag
    31861 ccggaatccg aacccgaact cggcgttgtc ctcgaagagc gagttggacc aggccggccc
    31921 gcgcccctcc ttgttgaccg tccacggcgt cgtgggcagg ttgccgccgt agatggagga
    31981 gcagccggtc gcgttggcga tcagcgcccg ctcgccgaac aggcgggaga ggatcgtcac
    32041 gtagggcgtc tcgccgcagc cggcgcaggc gccggagaac tcgaacagcg gcagggtgaa
    32101 ctggaagtcc ttgatgctgc ccaggtccag cgcgccgcgg tcggcgtccg gcaggctgtc
    32161 gaagaacttc cagttcgcgc gctcggcctc gaggatcggc gccttctcgg tcatgttgat
    32221 cgcgcgcttc gcgggattgg ccttgtcctt cttcgggcag acgtccacgc agaggccgca
    32281 gccggtgcaa tcctcgaccg tgacctgcac ggtgtagcgg cggcccggct gctccttgct
    32341 cttccattcg acgctcttga acgaggccgg ggccttggcg agctgcgccg gatcgtagag
    32401 cttcgagcgg acgcaggcgt gcgggcagac caggctgcag ttgccgcact ggatgcacag
    32461 gtccggctcc cacgcgggca cggcgagcga gatgttgcgc ttctcccact tcgtcgtgcc
    32521 ggtcggccag gtgccgtccg cgggcatcgc gctcacgggc aggtcgtcgc cgcggcccgc
    32581 catgatctcc gccgtgacat gccgcacgaa ctccggcgcc tcggccgcga ccacgggcgg
    32641 catgtcgtgc gcgctcgtcg cctgcgcggg gacctggatc tcgtacaggt gggccagcgt
    32701 ctggtccaca gcctcgtagt tcttgcggac gatctcctcg cccttcttgc cgtaggtctt
    32761 cttgatcgat tccttgatct tcgcgatcgc ctcctcgcgc gggaggacgc cggagatggc
    32821 aaagaagcag gtctgcatga tcgtgttcac gcggctgccc atgcccgtgt ccctggcgac
    32881 cttgtagccg tcgatcacgt agagcttcag cttcttgtcg atgatctgct gctgcacctt
    32941 gcgcggcagg gtgttccaga cctcgtcctt tccgtgcggg ctgttcagca ggaacgtcgc
    33001 gccgggcacg gcgtacttca gcatgtccac ctgctcgagg aagatcgcct ggtggcacgc
    33061 gatgaagttc gccgccgaga tcaggtaggg cgcgtggatc ggctcggaac cgaaccgcag
    33121 gtgcgacacg gtccgcgagc cggccttctt cgagtcgtag acgaagtagc cctgcgcgtc
    33181 catgtccgtc tcttcgccga tgatcttgat cgagttcttg ttcgcgccga ccgtcccgtc
    33241 cgcgccgagg ccgaagaaca tcgcgcgcac ggtcttgtcc gactccgtgc tgaacgccgg
    33301 gtcgtagtcc aggctggtct tcgccacgtc gtccgtgatg ccgacggtga aatgattctt
    33361 cggctgggtc ttcttgagtt catccagcac ggccttgacc atgcccgggg tgaactcctt
    33421 ggacgacagc ccgtagcgcc cgcccacgac cttcggcatg gcggccagcg agcccgcgcc
    33481 aagggcctcg gccagcgccg tgacgacgtc ctggtacagc ggctcgcccg cggagcccgg
    33541 ctccttcgta cggtcgagca cggcaatcgc cttcgcggtc ttcggcagcg cggccagcag
    33601 gtgggcggcc gagaacgggc ggtacaggcg gacgatcacc acgccgacct tgtccccgcc
    33661 gttcttccgg aggtagttca ccgtgtccag cgccgtctcc gcgccggagc ccatgatcac
    33721 gagcacgcgc tccgcgtccg gggcgccgac gtaatcgaag aggtggtagg cgcgcccggc
    33781 gaccttcgcg aaccggtcca tggccttctg gacgatgccc ggcgcggcgg cgtagaacgg
    33841 gttgaccgtc tcgcggccct ggaagtagac gtccgggttc tgcgccgtgc cgcgcatgac
    33901 cggccgctcc gggttcaggg cgcgcgcgcg gtgggcgatg atgagcttgt cgtccatcat
    33961 ggccttgatc tggtcgtcgt cgagcagctc gaccttgttc acctcgtgcg acgtgcggaa
    34021 gccgtcgaaa aagtggacga acggcacgcg ggattcgagg gtcgcggcct gggcgatcag
    34081 ggccatatcc atgacctcct gcaccgagga gctggccagc agggcgaagc cggtctggcg
    34141 ggtcgccatc acgtccgagt ggtcgccgaa gatcgaaagc gcctggcagg cgagcgagcg
    34201 ggcggagacg tggaagaccg tcgaggtcag ttcgccggcg atcttgtaca tgttcgggat
    34261 catcagcagc aggccctgcg acgcggtgaa ggtcgtcgtg agcgcgccgg tctgcagggc
    34321 gccgtggacc gcgccgctgg cgccgccctc gctctgcagt tcggccacga ggggcaccgt
    34381 gccccagagg ttcgtgcgcc cctcgctgga ccactggtcg gcccactcgc ccatgttcga
    34441 ggagggcgtg atagggtaga tggcgcagac ttcattggtc ttgtgcgcga cgtaggcggc
    34501 cgcttcgttt ccgtcaatga tcgtgatgtt cttgctcatt tccacacttc tccgttttct
    34561 ggctgcgcgt gaaagtcttc ggaattgaag atttcaggac aaaaaaacgg tgtaccactt
    34621 accacaagcg ccccgcccag cgcaagaacc gtcttgggtt ttttcggagt tgaaccgcgg
    34681 aggtcgcaga ggacgcggag gggcggggag ttggaaccgc gaaacgggca gggaaacagg
    34741 tgcaaggggt agaagttggt tggcacgcaa acaggtcagc cctctgcgcc ctctgcggtg
    34801 aaatctcttc ccagtgcttg gaaaagtatg gtgcattttt cccaaacatc ggaaaatcct
    34861 gaccccatgc ccctatcggt aacaacaccc tgggccctcg gcggcctgcc gctggccacg
    34921 cgcgcggtcc tggcgccgat ggccggctac acggacctgc cgttccggcg gctggtccgg
    34981 tcgttcggcg gcgtcgggct ggccttcagc gaaatggtca acccggacag catcctgcgc
    35041 ggccgcagcc ggaaggtgtc ggccctggtc gccaccgcgc cggacgaccg gccgctcggc
    35101 tggcagatct acggcacgaa gcccggcctc ctcgccgagg ccgcgcagtg gttcgaggcg
    35161 cggggagcgg agttgatcga cgtcaacatg ggctgcccgc agaagaagat ctgcagccgc
    35221 ggcgcgggcg ccgcgctcat gaaaacgccg gaactcgccg tcgagatcgc gcgccgggtc
    35281 gtggagtcgg tgaagatccc cgtcacggtc aagatccggc tcggttggaa cgcgccggat
    35341 gccgccgcgg agctggcccg cgggctggag gccgccgggg cggcggcgct gacggtgcac
    35401 ggacgcaccc gcgcgcagcg gttctcgggg gccgtggacc tcgacggcat ccggcaggtc
    35461 gttgaagccg tgcgtgtccc ggtgatcgcc aacggcgacg tgttcacgct cgccgccgcg
    35521 cgggagatgc tagagcggac cggcgcggcg gcgatcatga tcgggcggca cacgttgtcc
    35581 gagccgtggt tcctgcgcga cgtcgcccgc gacctcgcgg gcgagccgcc gcttgcggcg
    35641 ccctgtttcg cggagcggct ggcgatgatg cgccggctgt acgcggacca catccaactc
    35701 tacggcgagc ggttcggcgt gtcgctgttc cgtcgctgga tcccgcgatt tgccaagggg
    35761 atgggcgttc cgcgcccgat gatgatcgcc ttcctccagg ccgccacgcc tgccgagtgg
    35821 gaggcgcgga tggcggaggt cgagcgcggg gccggggtat gaaagacggc atggcggggc
    35881 ggcgcatcct tcttttgctg gtgatcgggc tggcggcctg cgcgcgcgcc gactggccct
    35941 tcgcgccgcc cgatcccggg cagtcctggg cgcaggatca tgtcatcgtc cggttccgga
    36001 cccgcgccgt ggcgcagcgg ttgggccggt cgctcgacgt gaatccgggc gcggcggaaa
    36061 aggcactggg cctgcccgcc ggcgccgcgg tggccggcgt ggcctcgctg ctccgcgggc
    36121 tggagccggg gctgacacag caggcggtca ccaaccggta ggaacgaacc gaccggcgct
    36181 gcttttactt tcgaggccgg gccacgcgcg cgcggatgac ggcctggatc tgctcctcgg
    36241 accgcaggag ctggtcgccg gcgacgattt cctcgacgat gaccgggatc agcccgctgt
    36301 tcagcccgag ggccatggcg aaggatacgc acagggcctt ctcctgcgcg ccgagcacgc
    36361 cgtcgatgat catcatgtcc accaggtcgt acagttgctg gaatcgcgag gcttcgtcct
    36421 tggggatgga gagatgaacg cgatcgggat gggcgaggac ttcctcgacc tgggcatccg
    36481 agaggcccca cttacgggcc aggaaattca gcaggtcccg ttccgcgccg gccagcttgc
    36541 cgtccctcaa cgccacgagg gccaggtttc ggaaatgact catcttctcc gccgagctcc
    36601 attttccgcc cgcgtcgccc atgccgcctc ctcctggttg gaaatcgcgg cgagcctaac
    36661 acccgcgtcg ccccgggtcc agccctgaac cttccttccc ttcccggttg acttcgcggc
    36721 ggaagctgct acaagggcag ggtcgcagcg aaaaacatac ggaaaaacgg ccatgaaacg
    36781 aacgtatcag ccctccaaag taaagcggaa acggacgcac ggatttcgta gccggatggc
    36841 tacgaagaat gggcgcaagg tgctggcgcg tcgccgccag gtcggccgca agcggctcgt
    36901 ggtttgacgc cgcccgccgc gggggaatca tgaccgatgc cgcgggcagc cggcgcgctc
    36961 cggacgcggt cctgggccgc gagcggcggc tgatccgttc ggcgctgttc cgggaagcct
    37021 acgaccaggg ccggcgccaa ttcgggcgct gcatggtgct ttggttgcgg tcgggcgagg
    37081 gcgcggccct gcgcctgggc gtggtcaccg gccggaaagt cggcggggcg gtgcagcgga
    37141 accgcgcgcg ccggcgcctg cgggaagcat tccggcggaa ccggcaccgg ttcgcgggga
    37201 cgtgggacgt ggtcctcgtg gcgcgccggg cggccctgga cgcgtcgtgg gacgatttgt
    37261 cggccgagtt gctctcgctg gcccggaagg cggggctgat gggcagcgag gacgtataga
    37321 tcagcggaaa gcgttagaag taataaactc ggaaggatgg aacaggagtc aacaaaggta
    37381 acggagaggg ttcgcctccg ttttttctgt tatctcctgt tccggtcttc ccgttgacca
    37441 tgaagttcct tttgatcatg ctgattcggc tctaccgggt cacgatttca cccctgctcg
    37501 gcccctgctg ccggttcgag ccctcctgct cggtctattt catcgaggcg ctccagcggc
    37561 acgggttctg gcgcggctcg atgctcgggg cgcgccgcct gctgaagtgc catccgctcc
    37621 accacggcgg gttcgatccc gtgccggcct cctcttccct tcgttgtccc ggagttgatc
    37681 gatgaaaaga aacgacatcc tgatcctcgt ggccctcttc gccctgtggc tggcctggcc
    37741 ggcgctcgac cggcacgtca tcaagaagta cttcttcccc gccccgccgc cggcggccgt
    37801 cgaggaggcg cccccggccg acacggcctc ggagccgccg gccctggccc cggccgaatc
    37861 ggcgcccgcg gcggaggcgc cggccccggc cgcggaagtc gtcgcggagg cggagccgga
    37921 agacgccggc ccggaaacca cggccacgct ggaggacggg ctgctccgcc tggcgatctc
    37981 ctcgcgcggc gcgtcggtgg cctccgccga gctcaagcag tatcccctga ccgccgaggc
    38041 ggacagcgcg ccggtgctcc tggatttctc ctcggcgcgc gcgctggcct acggcggcct
    38101 ggcgggcctg tccgaggcgg tgaatttcag cctggtgtcc caggccggga atgaaatcgt
    38161 cctggagcgc accgcggctt ccggcctcca gcttcaacgg actttccaac tggaggacgg
    38221 ctacctgctc aaggttaccg accggttctc caaccgcggc ttggcggccc tggaagtgcc
    38281 gggccacgtc atgcacacgg gcccgatgcc caatttgccg ggcgagagcg cgatgaaggg
    38341 cgtggtgaac cagggcgtgg acacgctctc gccgggcggc gagggcgtcc agcactgggg
    38401 cgccaagctg ccgcggtggt tcgaggaggt gcggaaggag aaggggcttc cccgcatccc
    38461 cgagcggatc gagtggccgc tggatacccc catggaatgg gtcgcggcca agaacaagta
    38521 tttcgcgcag gtcgtggtgc ccgagggcgg ggccgagaag gccgtggtcg tcgcgcggcg
    38581 cgagttgccg gaggccgagc ggcaggaccc cgccgccaag ccccgcgggg cggtcctcgc
    38641 cgacgtggcc gtgtcggtcc agttcggcgc gacggcgctg gcgcccggag aggcgttcac
    38701 ccggacggtc cagtgctata tcggcccgaa gaagttcgac gtcttgagcc gccacgggca
    38761 gcacatggtg gacgtgatgg agttcggctt ctgggcggcg atcggcaaga ttttgctcca
    38821 ggtcatgaac ggcatccacg cccacctgtg gccgcacaat tacggcatcg cgatcatcct
    38881 gctcacggtc ctcatccgcg tcgtgttctg gccgatcacc cacaagagca cggagagcat
    38941 gcggaagatg caggagatcc agcccctggt caccgccgtg cgccagcagt acaaggacaa
    39001 cgcccagaaa cagcagcagg cgatcatggc gctctacaag gagcacaagg tcaatccgct
    39061 gggcggctgc ctgccgatgc tcatccagat cccggtcttc atcgccctct tcgtggtctt
    39121 gcgcagcgcc atcgagctgc ggttcgcgag cttcctgtgg atccgcgact tgagccagcc
    39181 ggagaacctg ctggccggga agctgccgat cgcgctgaac ctgctgccga tcatcatggc
    39241 ggtgaccatg gcctggcagc agaagctgac cccctccggc ggcgatccga accagcagaa
    39301 gatgatgatg ttcatgccga tcatgatgct ggtgctgttc tacaatttcg cctccggcct
    39361 ttcgctgtac tggacgacga accagtgcct gatgatcgcg cagcagttga tcgcccagcg
    39421 gcgcaagaag gcccgggagg cggccgcggc ggcgcggtag tcgaggagga acgccatgag
    39481 cgaccaggac acgctctcgc aggtgcagga cgaggcccgg cagacgctgg gcgaattgct
    39541 ccggctgacc gggttcgacg cgaaggtcga ggcctccgcg ccggcggagc aggaggtgct
    39601 gctgcgcatc gagtgctcgg acgcagccca gttgatcggg cgcaagggcc aggtcctcga
    39661 cgccctccag ttcatcctcg gccgcatgct cctgcgcaag gccgggcggg acgtgcattt
    39721 cgtcgtggac gtcggcgact accgcgagcg ccgcaaggag caggtcctgc gcgaggccat
    39781 ggccgccatc gaggaggtcc ggaagaccgg gcggcccgtc aagctgcccg tgatgtccgc
    39841 ccacgagcgg cggctggtgc accacctggc cgccgagacg cccggcctgg aaaccttttc
    39901 cgagcccacg ggcgaggaag gccgcaagcg cgtggtcatc agcccggtcg gcgaatccgc
    39961 gccgccggcg tgaagccgtt ggttcgcgcc gtcgccgggc aaaaaacggc ccgcccccgc
    40021 gaggggacgg gccgtcttgt ttctcctgct cttccaggat cagaactgga accggccgcc
    40081 gaggccgaag aacaggtcgt cgatgtgcgc gagctcgccg tagagcgaga actgctcgtt
    40141 cagggcgaac agcgcgccgc cggcgatggc gacatcggtc tcgtcctcgc tggcgtcgcc
    40201 gacgccttcc caatcgatct cgacatcgcg gtagcacatg ccgaggaacc cgtagggcgt
    40261 gaactgctcg atggtcttgc tgacgagggc cccgaagttg acgaacagcc agtcggcgtc
    40321 ggcgtcatcg gtgacctcgt agctcgccat gccgatggag ccgcgaaggg cgacgtcgac
    40381 gggcaggtcc agggggagcg tgtattgccc gccgccctgg taggtgaacc cggtgtcgcc
    40441 ttcgtccgga tcgagcaagc ccaggtcggc gaacagggcc agcccttcca tgacgccgaa
    40501 ggtcacgcgt ccgccgtacg cattggcgtc atcgccgacc acggcgccgg ccgacccgcg
    40561 aaccaggcct gcctcgggcg ccgccgcgcc gtccgcgtag gggagtcctg caaactgggc
    40621 catcgccggg gcggccatgg cgcctaccat tgccagcacg atccacttct tcatgctttc
    40681 tccttctggt tatgcttccc atcagttgtc atttccggat cggatcagca actaatggtg
    40741 ctattattaa tagcctacca catgatatgg tggcccgtca acgaattaag tggacttttt
    40801 tttagcgaat gcggccgccg cgatcggtgc gggattgcct gccggcccgg acgttggtaa
    40861 aagtcagcgg tattcggcgg gacgcgacac ggctcggaat cgatggatga agacgggaag
    40921 aaagcacatg aagctcgtgt ggctggcggc atggggggcc ggcgccctcg cgccggccgc
    40981 cggcgcgcag accctggcgc agcagctcct cgccggctac gacgcggtgc agagcgtcag
    41041 ctgcgaagtg cgccgggaca cggaaacggt cggcgggaac tccgcgcgcg cgttgagccg
    41101 cgtctacttc cagaagccgg accggctgca cgtggagaat gtcacgcccg tccagcgccg
    41161 gatcgtggcc gacggcgaga cgttcttcag ctacatcgag ggcgacccga agggcttttc
    41221 ccggccggtg tcccagctca acgaggagat gaccatccag ctccgcaaga ttccgggcac
    41281 ggccatggac cacctgttga aaataggcga tgcgccggaa accgtcctcc cggcggcgga
    41341 gggcttcccg gtccgacgcg gctacgagaa gggccggctg ttcgtcgtcc tctccatgga
    41401 cccggagaac cgcctggccc ggatcgagtt cttcgattcg ccggaaatga agaaccggac
    41461 cgcccagtac gactacagcg ccttccagcc ggccgcgggc ggagcggtga ttccctgcct
    41521 gcaccggggc gtgctgtgga gcggcggcgt ggagacccgc gagacggtgc gcatcagcaa
    41581 cctggccgtc aaccagcccg tccccgggag ccttttcgtg gccggcccct tcttcgaggg
    41641 ggtcgatttc gtggacgatt tcggcaacat atacgaataa cgtcttgccc gcgcaaaaaa
    41701 acttgcgcgc ggcatttgta aacgtagagt agggcgaatc aggaaggaga cagcatgaag
    41761 aaagtcattg tcggcatgtt gattgcggcg tttgtcctgg cgggattggg cacggtgcag
    41821 gcggcggaga agggcgcggg ccgcgggggc ctcatgggct tcatcgcggg ttgctgcttc
    41881 ggtatccggg ccggctcggc ctacaacgac ggcaaggaaa tccactggcg cgaatgggtg
    41941 cggctggttc cggtcgtcag catcgtcgtg gcgatctggg acggcatcga cggcgccaac
    42001 ggcgtgacca gcaaggatta cgccaagcag tacggcgaga cctactacta agtccggccg
    42061 taccgtcatg cgtacgagga ggattccggg tggatggccc gggatcctcc tttttttcgc
    42121 cgcggcgcgg ctgggcgcag ccggggccga tcttgcgcag gagctcttcg acgaaggccg
    42181 ctgggccgag tgcgcgcgcg aaagccgccg cgtcctcctg tccgatcccc ggaacgaaac
    42241 ggccgcgctc catctcgccg tcgcccggct gcgcctcggc ggaaccgatg aggaaccgct
    42301 ggccgcgctg acgtccgccc gcgatccgga aatccgcgcc cgcgcggcct acgagctggg
    42361 ccggctgaat tgggccgcag gcgatcccgc cgccgcctac ccgctgctgc gccaggcctt
    42421 cctggaaacg cgatcccagc ccctgtccct gcggagcggc tgctcgctga acgagctgct
    42481 ccgggctttt ccaaaccttg gaaaagacga ccttgccctc gtccaatcat tggaaacctg
    42541 ccggccgctc tggagccgcg cgctgcgcgc ggaatgcgcc gcgccgcgcg cccgggcgga
    42601 tcgcggcgga tggctgtccg cgcccgggaa gtggctggtg tcgttctacc gcacccagat
    42661 tcgccccgcg atcggcgcgc gctgctcgct ggagccgagc tgctcggcct acttcctcgc
    42721 cgccagccga gcgcacggcc tgctcgggct gcccatgatg gccgaccgcc tggtccgcga
    42781 gccgtccgtc gtcgccgagg cgcgccggcc ggtcccgggc gacggggcgc cgcggttcgc
    42841 cgacccggtg tccgatcacg atttctggtt ccacggcggg ggagggaact gatgggcgcg
    42901 cggacctgga gcgccgttgt cgccctggcg tggctggttc cggcctgcgc cgcggcgcag
    42961 gactcgccgg ggctttccct ggccgtggag ctttcggcgg agggcgacca cgccggcgcg
    43021 gccctggagt accgccggct cgcgggcgac gcgccgtccg gcgaggcccg gggcggctgg
    43081 tactgggcct cggccttcga gtactggcgc gcgggcgaat acgaactcgc ggaccggatg
    43141 ctgaaccgcg ccgaggacgc gcagcccgcg ctcggcgcgc ccgccctgct gctgcgcggc
    43201 gagaacgccc agtcggctcg gaagtgggcg gaaagcgatt tctatttcga gagcgtcctg
    43261 cgcggcggga ccgcggatga cgagaaggcg ttcgccagcc gccggctcgc cgccggccgg
    43321 ctgcggcaga aggacgtgac cggcgccggc gccgcgctgg acctggcgcc cgttccccag
    43381 gaagccgccc gcgccgcgct ggcggaatac aaccgcggcc gcgacaagag cccgctgacc
    43441 ggcggcctgc tcgggatcgt ccccggcttc ggctacttct acgcgggcga atgggccagc
    43501 ggcgtccgct cgatcctgct caacagcctg ttcctcttcg gcatggtgga cacggcggac
    43561 cacgaccagt ggggcgcgtt cgccgtcatc accttcttcg agttcacctg gtactccggc
    43621 agcatctacg gcggcatcga cggcgcgcac cgcttcaacc gcgaccggct ggaaaccgcg
    43681 gcagacggca tccgcggcgc gtccgcgttc gagccggacc ccgccgcctg gccggcggtc
    43741 cgcctgaatt tccggttctg aaatcagggt tgaactcccg cggggctgtc cctaccttcc
    43801 cgatatgcgc cgtggccttc acctctgcct gctcctcgcg ggcctgcttg cggatcccgc
    43861 gcgcggcgcc gcgccggagg agacgctccg ctacgagttg gtctggggct tggtccgggt
    43921 cgggaccgcc gaggtgacga cgcggctggt cgaggaggac gggcggccgc tgctccgctt
    43981 ccgcttcacc gcgaagagca accggtgggt ggaaaagatc taccccgtcg aggattgcat
    44041 cgacagcttc atcgagccgg aaaccgggct gccggtgaag ctggtcaaga agacttccga
    44101 gggctggttc cgctgcgacg acgaactgcg gttcgaccgc gagcgcggcg tggccgcgtg
    44161 ggtcgaccat gtccgcggca cgaacacgga gtatgccgtc gcgccgggcg cgcacgactt
    44221 cttctcgctg atgcgcagca tgcgggagct cgacctgcgg cccgggcgcg aggaccgcgc
    44281 gaccatcgcc gccgacgacc ggacgcatga cctggtgatc cgcgtggagc gggaggagac
    44341 cctttccctg cgcggcgcgc gggtgcgggc ctaccggctt cgtatcgcca gcgagaagga
    44401 agggctcttc gtgcgcaaag tacccggggc gatctggatc acggccgagc ccccgcgcac
    44461 ggtggcgcaa atgcaggtgc gggtgcccgt cggcagcgtc cgggccgtgc tgctggacga
    44521 gtcgaaatga agaaatccct tgtattcatg gcgtgggcgc tggccgtggg cggaagtttg
    44581 cgcgccggcg ccgcgtcttc cgcgcccgcc ggcacgggat cgttttcccc gctggcccgg
    44641 cagctctgga aggccggcgg gccgggctgg gtccgctcgg aaaaaaacgc gttccacgtc
    44701 tatggccggt cgccgtggga ggtgggggat gtccgggccg aggcggagtc ggcgtggccg
    44761 gcgatcgagg agagcctggg gcccgatccc gcgccgcgcc cggcgcggat tttcgtggtc
    44821 ggcgaccggg ccctctgggg ccgcgtgctg cgcggcgcgg accgccgcaa ggacagcctg
    44881 gcgatccagg ccggggacga ggtattcctg ctgcgcgagc cggacgccgt cgccgacccg
    44941 ggccgcctgc cgcacgaact ggtgcacctt aaactgtggc ggcgtttcgg cgaccgcgtg
    45001 ccgctctggc ttgacgaggg gttggcctcc tatctaggtt gggccgcggc cgcccgggtg
    45061 gcgcgggccg gccgtgcgcc gctggaccgg cggctgccgc tgtacgaggc cgcccaactg
    45121 cagacgatgg aagaggtcca ggcggtccgg tcgtactcgg ccatgggccc ggctttttac
    45181 cggcaggcgg aggaaatggt ggcggccgtc gcgcggcgcg cgggggacgg gcgcctgggc
    45241 gaattcgcgg aagccgtggc gcttgcgggc gtttcgtggc aggattacct gaagcaactg
    45301 ggcctgtccg acgtcgaaag ggaagacgcg gaaaaggaca tgcgggatcg cgtgaccgga
    45361 aaggcgcatt gaaggcgggc gcggaaaaat caaaaaggcc ggccaagccg gccggcggcg
    45421 gcgcggagcg aaaggcgatc tacctcgtct acggcgagga tgagtaccgc gtgcggacgc
    45481 gcgcgaagga actggtggac tcgctctgcc cgccggccga ggcggcgttc gggctggaaa
    45541 tcatcgacgc cgccgtcgag aacgccgccg agggcatcct cgcggtgcgg cgcgccctcg
    45601 aggccctgcg gaccgtgggt ttcctgggcg ggcggaagac cgtctggctg cgggacgcca
    45661 cgctgttcac caaggatatc gccgaggacc tcaagcgggc ggtggaggaa ctcacggacc
    45721 agatcaaggc cgggctgccc gtgggtcaga cgctggtggt cagcgcgctc aaggtcttcc
    45781 gcggctcggc gtttttcaag gcgtgcgagg ccgcgggcac ggtggaggag tgcgcgaagc
    45841 cggacaagcc gtggcagcag gcggaatacg cccggggcca ggtgatggag gaattccggc
    45901 ggcacgggct gaagcccgtc ggccgggcgc tcgatctgtt cctggaccgc gtcgggatgg
    45961 acacgcggtc gatcgtccag gaggccgaga agctggcggt gtatctcggc ggtcgcaagg
    46021 acgcgcgtcc ggaggacgtg caggcgatcg tgtcgctctc gcgcgagtcc gcggcgtggg
    46081 acctggccga cgccgtgggg gcgcgcgacc tgcagggcgc gctgaagata ctgcgccagc
    46141 tcgtattcca gggcgagagc gccatcggcc tgctccacgc gctggagttc cggttccggg
    46201 agatgatcgt cttccgcgag gcgctggacc gcggctgggc gcggccggta ggcgaagggt
    46261 acggccgcaa gctggcgtgg cgggagggcc cggagatcga cgccgcgctc aaggccctgg
    46321 gcaaggaccc gcgcgagggg cactggttcc gcggcatgaa gctggcggag caggcgcaac
    46381 ggttcagcgc ggcccaactg cgggacggcc acgcgatcct cctgaaggcc cacgaggcga
    46441 tggtcagctc gtcggtgccc gacgcgctgc agatggaact gctgctgttg aagctgctgg
    46501 ctcccagccc gcgggacgct taaatggagg gcggtcggcc tcgcccgcca aaatcggcga
    46561 tggaggccca ccatcgccac gcctgcgggc aagccgtcgc gcttcaaagc cagagcagga
    46621 cacggcgttg cgaacgagcg cgcttttttc ttgcgatacc taaaggggag gatgaccatg
    46681 caaacaaggg caaatggtgg ggagaggcaa atggggtcag ccccaaaaat aaagaataat
    46741 agacattccg gaataatgta ttcatgatct aaagcatggc tagacccctg cgattgcagt
    46801 ttcccggcgc gatctatcac gtgacatttc gaggaatcga gcggcgaacg atcttcaggg
    46861 atagccggga ttacaagcgg atgctcgaag cgctggagga gatggccgac cggcacaggg
    46921 tgcgcgttta cttggtctgc ctgatgacca accacgccca tttgctgctg gaaacgcctc
    46981 gcgggaatct gtcggccttc atggggcagt tgctgaccag ttacacggtg tatttcaatc
    47041 ttcgccaccg gcgggtcggc cacttgaccc agggccgata taaggcgcag gtcgtggccg
    47101 gtgacgagta cctgttaaaa ttgagccgct atgttcatct gaatccggtc catatcaagg
    47161 gcgcggaaga atggccgatc gaggagcgtg tgagacggtt gcgggcgcat ccatggagca
    47221 cgtaccgaag ttacgctggc ttggagcgag cctggccctg gatcgagtac ggaccgatcc
    47281 gcgcactgat agggaaaggc gccggcggcg cggcatatcg tggctttgtg gaggaatgtt
    47341 tagcgcggag cgaggatgat tttcgagcgg tctatcgtag cgcgcggctt gctctagggt
    47401 cggaggaatt ccagcgggaa gtggagcaaa agcatatagc gatgggccga atgcggcgac
    47461 ggaaggagga tgtttccttt cggcggttat tgggccggtg cgaaccgaaa gccgttctgg
    47521 agggagtagc ccgagcattc ggtgtgaagc ccgaggattt gcagaaacga aggcgcaacg
    47581 ctgacttccg tgcggccgcg tgctggtatc tgcatgctca aggcgggctg acccagaggg
    47641 atgcggccat gtgggtgaac ctgaaaagcg gcgttgccgt tgcctatcaa atcagacgct
    47701 ggcaaatcat gagtcgagaa aagcgccttt gtagcgccct tgaaaaacta ccggccgatt
    47761 ggagccggtg aaatgttatt ctttattttt ggggctgacc cctttttccg ggacatatca
    47821 agggcgcgga agaatggccg atcgaggagc gtgtgagacg gttgcgggcg catccatgga
    47881 gcacgtaccg aagttacgct ggcttggagc gagcctggcc ctggatcgag tacggaccga
    47941 tccgcgcact gatagggaaa ggcgccggcg gcgcggcata tcgtggcttt gtggaggaat
    48001 gtttagcgcg gagcgaggat gattttcgag cggtctatcg tagcgcgcgg cttgctctag
    48061 ggtcggagga attccagcgg gaagtggagc aaaagcatat agcgatgggc cgaatgcggc
    48121 gacggaagga ggatgtttcc tttcggcggt tattgggccg gtgcgaaccg aaagccgttc
    48181 tggagggagt agcccgagca ttcggtgtga agcccgagga tttgcagaaa cgaaggcgca
    48241 acgctgactt ccgtgcggcc gcgtgctggt atctgcatgc tcaaggcggg ctgacccaga
    48301 gggatgcggc catgtgggtg aacctgaaaa gcggcgttgc cgttgcctat caaatcagac
    48361 gctggcaaat catgagtcga gaaaagcgcc tttgtagcgc ccttgaaaaa ctaccggccg
    48421 attggagccg gtgaaatgtt attctttatt tttggggctg accccttttt cctgcgtgta
    48481 tcttttcccg ctcatctccc gcctcccttt ccgggccgcc agaaggggag catactaaag
    48541 atcgccctgt ccagtttttg gggaggacgt caggggcaac tacaccccca atgtcgggcc
    48601 atacccggtc agcggtgaca gcctggcgta tcactacgaa aagtcgggaa aacaggcgct
    48661 gactttcctc attgaaatag ccgatgagtt tcgtccggcg aatgcaacag agcatgccaa
    48721 cgctgtgctt ccagggcaac tcttcatgat gcttgcggcc gtggacagga gtttcgcatc
    48781 aaagccgacc gcacgattca ggaagccgca atgacgcact tcaatcacaa ttcgggggtt
    48841 ctgcaatgaa gacaagcagc ggctattccc ggcgagtgct tcttgccgga ggcggcggct
    48901 tgagccaagc cgccctacct aaccagcggg gcgagcgggg cgggtcaagc ctaaactatt
    48961 aactattggc cgtcggagag agatgaaaac aaccgaacaa ggctattccc ggcgggcgtt
    49021 cttgaaactt gcggcggccg gcgcggcggg agcgggggtg gtgggttggc tgcggttcac
    49081 aggaagcgag cgggtagttg tctgctctaa gtgcgcggcg gaattcgcgg aaggacatca
    49141 ttacaaggcg gggcctgttc gcggggtata ctgcccgaat tgcggcgtgg agctgacgcg
    49201 gttggaatac gatttgcggc aggatgccat gtttgaagac ggggataagg cgcagagcga
    49261 agaggcaagg agaaagtgga actatggcca ggtgcctttc ccgaatccga agttggtaag
    49321 ccggacggac aagccggcga ttgttctttc agaaatcaag atttgagggg cggtgcgcga
    49381 tttagttctc cgcaagagcg gggggtgtgg cgattcgcca tgcgcaacgt taaccagaac
    49441 agcgccaagc tctcggccct agccaagcac gggaccgaga gccgaggcgc gcctatacgc
    49501 gcagagaagg taaaccgcgc cgcccctcat ccgttatcaa tgaggttaat ataggccaga
    49561 gacgggagcg ggtagggtgc gctggcccag cgcgccgccg ctcgttgcga gtgcttcttg
    49621 ccggaggcgg cggcttaaac caagccgccc tacctaaccg gatgagtgaa gtagttcttt
    49681 cagaatggaa gatttgaggg gcggtgcgcg atttagttct ccgcaagggc ggagggtgtg
    49741 gcggttcgcc atgcgcaacg ttaaccagaa cagcgccaag ctctcgaccc tagccaagcg
    49801 cgggatcgag agccgaggcg cgcctgtacg cgcagagaag gtaaaccgcg ccgcccctca
    49861 ttatttttaa accgaatatc gaaatccgaa acccgaaaca aattcggaag ccgaatgaag
    49921 gaatgaccaa aacggtgaac ttgaaccggc gcggggtgat ctatgcagac cgcaaacaaa
    49981 tacctggccg ttgaaaattt cggcctgctc cagcggcagg cgggggtgga tcccgagctg
    50041 gcggagttgg tttgcgcctg cgcccgcttg ttcagcgatt cccaacagcc ctggcttttc
    50101 tatgatgccg gcgtcagtcc ggcggattta aagcatgact tcctctgcgg ctggctggcg
    50161 aagaagaatc cgcaaatcga cccccgcttg ttggagttcc tgacgcgcta tagccggttg
    50221 atggcggcgc gacggctccc ggtgattttc agcgtgggtc attttgcccg gaagcatgac
    50281 ttgagcgagc ggcatgtccg ctggatcagc cgcaatgtcc tgcgcttcta caaggaattc
    50341 aatatcccga aatcggatgg cggcgcccgt caaatcctgg cgccggaggg gaagttgctg
    50401 acgctccaac gatggatact ccggcatatt ctcgataagg cccggcctca tccagtggcc
    50461 tgcggcttcg tgaggggacg ctccatcatg gacaacgtgc ggccgcacat cgggcgcgcg
    50521 gtggtcgtcc gggtggactt gaaggacttc ttcccgtcca tcacccatgc ccgaatccgc
    50581 aaggtcttcc aggggctggg ctatccttat cgcgtggccg tgttgttgac gaatctgtgc
    50641 acggtgaatg ggcggttgcc ccagggagcg cccaccagcc cggctttgtc caacctcgta
    50701 ttcttgaaag cggatcggcg ctttacgggg ttggccgaga aaatgcagtt ccgctattcc
    50761 cggtacgccg acgatctgat gttttcctcg gataatcccc ggcttgcgtg tctcatcccg
    50821 ttattcaagc aaatcgtttg cgaggaaggc ttcacggtca acgaggataa agtccgcgtc
    50881 atgcggcggg gccagcggca gcaggcgacg ggggttgtcc tcaacgagaa gccgaacgtg
    50941 cggcgggaac acgtccgccg cctgcgcgcg gcggtacatc gcctgcgaac gctaggcccc
    51001 gaagatgtgg ttctgggttc gcggcggggc ggtgcgcacg atccggaata cgtgctccgg
    51061 ggccatctgg ccttcctccg catggtcaat cccgcgcagg gggcggcgtt ggcggagcgc
    51121 ttgccggcgt agctggtggc gccaagcggt tttgatggtg agtccagttc cgcggggtgg
    51181 gggcgccccg gctcctgcag ggcggtctgc ccgtttctcc ttgtaagaac cgagatgccc
    51241 gtgacaatac cagcgcgatt tcaggccgcc gggcatgaca ggcggcggga aacatgataa
    51301 ggtccaccga ttcaatagcc gcggaatgag agcatgatcc tgatcctgca gcccaacgtg
    51361 gacctggagg gggcggagta ccgccacctg tccgacacgc tcgccctgct gccgaacgtc
    51421 cagacgcggg tccaccatgt ccggggcgcg cagcagacgc tgacggaaat ctacctgatc
    51481 ggcgacacga agctgctctc gatggaggac atgcgcaacc tgccgtgcgt cgagagcgtg
    51541 gtccgggtgt cgcaggccta ccgggtgatc ggccgccaca aggacgacca ccgggcgacc
    51601 tcgttcgagt acaacggggt gcgcttcggg caggacaacc tgcacgtctt cgcggggctc
    51661 tgcgcggtgg acaaccgcga gaacgtcgag cgcatgatga aggccctgcg ggaccacggc
    51721 caggtctgca cgcggatggg ggcctacaag ccgcggacga atccctactc gttccagggg
    51781 cacggcgcgc actgcctgcc gtacgtgttc gagctggccg ggcggtacgg catccgggtg
    51841 atcgcgatgg agatcacgca cgaggaccac atcgaccaga tccgggcggc gctcgacgcg
    51901 gcgggccggc cgacgggggt gatgctgcag atcggcacgc gcaacgtgca gaacttcgag
    51961 ctgctcaagc acgcgggccg gcagcaggag ttcccgatcc tgttcaagcg cgggttcggc
    52021 atcacgctgg aggaatcgct caacgcggcc gagtacctgg ccagcgaggg caaccagcgg
    52081 atcgtgttct gcctgcgcgg cgtgaagacg cacatgggcg acccgcaccg gaacctggtg
    52141 gacttcgcgc acgtgccggt ggtccagcgc ctgacgcgcc tgccggtgtg catcgacccg
    52201 tcgcactcgg tcggcgggcg cggccgggcg ccggacggcc tgctggagtt gttccacgtc
    52261 accgcgcagg gcgtggtggc cggcgcgaac ctggcgctgg tggatttcca tcccgacccg
    52321 gaccgcgcgc tcgtggacgg gccgcaggcg ctgcacatgg acgagctggg gcccttcctg
    52381 gaggacgtgc gcattgcgcg ggaggcctac gagcggcggc ttgggcgcat cgcggcggcc
    52441 ggggggcatg gcgataaagt acggtagggc gcgttggctc aacgcgccgc gtatggcggc
    52501 gggttcggcg aacccgccct accttgagaa gctgatgaaa aagcggcaca cagcggtagc
    52561 gatgctggtc ctgggcctgg ggctcgtggt ctgcgcccgc gcggcggagc cggtccccga
    52621 gctctggttc cccgtcggcg aaaagctgac ctaccggatc tactggggca tgttcaaggt
    52681 cggcatcacg acgaccgcca cgggctggat cgaggaggac gggcgcacgc ggctggcgat
    52741 ccggttccgc acgcgcacca accggatcct cgaaaagctc tatcccgtgg acgacttcct
    52801 ggaggcgatc atcgacccgg tgaccttcct gccggtccgg ttcacgaagc gcctcaagga
    52861 ggggcggtac cgcgcggacg agacgacggt cttcgaccac gcccgcggcg tcgcccactg
    52921 gcggtcgaac atgaccgaca agaagaagga cttcgccatc gagccggaca cgcgggacgt
    52981 catctcgttc atgtacttca tgcgctcgcg cccgctgaag atcggcgaca cggtccacgc
    53041 gcgcgtgatg gccgacgaga aactctacga cctctacgtc aatatccgcc gccgggagaa
    53101 cctcaagctg ccggagatgg gccggcggcc gagcgtcaag ctggagcccg aggcggcctt
    53161 caacggcctg ttcgtgcgca agggcaagct gtggatgtgg gtctccgacg acaaggaccg
    53221 gttctgcacc aagctggcgg ccaaggtgcc cgtggcgagc gtgtcgctgt ggctgaccaa
    53281 ggtggaggtc gagcccgacc gggtcgtgac cggcgaatcc gaagacgacg aggaactcga
    53341 ggtggacgat gagcctgcgt cctgaacgcg cgcgggaact gctggcggcc ttcccgcggc
    53401 agcgcatcct ggtgatcggc gacctgatgc tggaccgcta catctacggg gcggtccacc
    53461 gcatctcgcc agaggccccg gtccccgtgg tctccgttac gcacgagaag agcatgccgg
    53521 gcggcgccag caacacggcg tggaacgtcc gctcgctggg gggccaggcg gcggtcggcg
    53581 gcgtgctggg cctggacgcg gccggcgcgg acctcaaggc ggtgctcgcg ggcgggggcg
    53641 tcgggctcga gggcctgctg gagatggacg gcctgcgcac catcgtcaag acccgcatca
    53701 tcgccgaccg ccagcaggtc gtccgcgtgg actgggacga tcacgcgccc gtcgccgagc
    53761 cccgggcgag atcgttttgc gacctgatcg agcgcgaggc gcggcgggcg gcgggcatca
    53821 tcatcgagga ctacggcaag ggggtggtgc gccagccggt cgtggacgcc gtgctcaagg
    53881 tcgcgcgggc ctcgggcatt ccggtcggca tggacccgaa ggaggggcag gagctcgacg
    53941 tgcgcggcat caccctggcc acgccgaacc ggcgcgaggc cttccacctg gccggcgtgc
    54001 cggagacccg gccgcaggcc catccgctgg aggaccaggc cctgctgggc gtcagcgaga
    54061 aactgctgga gaaatggcag gccgcgcaga tcctcgtgac gctcgggccg cagggtatcc
    54121 tcctgcgcgg ccgcgcgggc gaactgcacc acgtgccgac ccgcgcgcgg gaggttttcg
    54181 acgtcagcgg cgcgggcgac accgtgatcg cgacctgcgt cctggccctg gccgccggcg
    54241 cgagcccgat cgaggccgcc gaactggcca actacgccgc cggcgtcgtc gtcggcaagc
    54301 tcgggacggc cacgtgctcg ccggaggagc tgatggcgca gatggggaag tgaatagtga
    54361 atggtgaatc gtatttcctg aatcctgaac cctgcttgcc cgccggagcc ttggcgaagg
    54421 ggggagtcct atgacccgca tcctcggcat catcccctcc cgttacgcct ccgtccgatt
    54481 tcccggcaag agcctggcgc tgatcgcggg caagccgctg gtccagcgcg tggtcgagcg
    54541 cgtccaactc gcgaagcggc tggacgcggt cctggtggcc accgacgatg agcgcatcgc
    54601 ggcggcggtc cgcccgctgg gcgtcgaggc cgtgatgact gacccggcgc tgccgtcggg
    54661 cacggaccgc gtcgccgccg ccgcgcgcgg gcgcggggcg gacatcgtga tcaacatcca
    54721 gggcgacgag ccgctggccg atcccggcgt catcgaccgg cttgcgggcg tcatggcgtc
    54781 cgacccgaaa tgggacatgg cgacgccggc ctgccggatg gaggacgggc cggaggtgga
    54841 ccagcccgcg atcgtcaagg tcgtctgcgc ggccgacggc gcggcgctgt acttctcgcg
    54901 ctcgcgcatc ccgcacatcc gggacgccgc ggatgcccgg ggcgccgggc ccgtctactg
    54961 gcagcacatc gggatttacg cctaccggca ggcggcgctg gagcggctgg tcgcggagcc
    55021 gccgtgcctg ctggaaaaat tggagaagct cgagcagctc cgcgcgctgc acctgggcta
    55081 ccggatcaag gtcatcgagg cggcgaagcc gggcttgggc gtggatttgc cggaggacgt
    55141 ggcgaaggcg gaacaaaggc tgaaagagga aggctgaagg atgaaagcag aacgtccaac
    55201 gcccaacggc caacgtccaa cttccaacct gaactctgct tgcccgccgg agtcccggcg
    55261 aaggcgggaa ccctggaatc tatttatggc tgcaacaaaa accatccgag tcgggaacgt
    55321 ccccgtgggc gggcgcgcgc cgctggtcct catcgcgggg ccgtgcgtga tcgagagccg
    55381 cgaaatgtgc ctggacctcg cggggcggct ggcgcgctgg tcgaagaagg ccggggcgcc
    55441 gctgatcttc aaggcctcgt acgacaaggc caaccgcacg tcgcaccgtt cgttccgcgg
    55501 gccggggctg acgcgcgggc tggagatcct ggcggaggtc aaggagcggt acgggctgcc
    55561 gctgctggtg gacgtgcacc acgagaccga agtgccgctg gccgcgaagg tggcggacgc
    55621 gctgcagctc ccggcattcc tctgccggca gacggacctg gtgctggcgc tcggcgagag
    55681 cgggcgcgtg gtgaacatca agaagggcca gttcctggcg ccgtgggatc tcgggcacgt
    55741 gatcgcgaag gtcgagagca cgggcaaccg gaagatcctg ctgaccgagc gcggcgcgag
    55801 cttcggctac aacaccctgg tggcggacat gcgcagcctg ccggtgctgc gcgagaccgg
    55861 ctacccggtg atcttcgacg ccacgcacag cgtccagtcg ccgggcgggg cgggggacca
    55921 cacgggcggg gacgggcggt ttgcgcccgc cctggcgcgc gccgcggtgg cgacgggctg
    55981 cgacggggtc ttcctggaga cgcacgtccg ccccgaaaaa gccttgtccg accgggaaaa
    56041 cgcgataccg tggtccgccc tcgacggatt gtggaaacaa ctgaaagcga tcgatgacgt
    56101 ggtggactga aagaatgccc ggcgcgctgc ggctggccgc ggcggcggcg ctcctgttcg
    56161 cggcgccggc ccgcgcgcag ctggtctcca ccggcgcgac gcagacggtc tccgggttca
    56221 aggtgccgga ctacgaccag aacaacaacc tcaagagcgt cctgtacggc gacttcgcgc
    56281 gcgtgcggcc cgacggcatc gccgagatca ccaacgtgaa gctggagttc tacaaggagg
    56341 gcgccgtgga gatgacggtg acctcgcccc ggtgcgtgta caaccagaag gacggcaccg
    56401 cccggtccga cgcggacgtg gagatcgtcc gggagggcct gaagatcacg ggcaagggtt
    56461 tctggtgggg cgcgaagcag gagcggtttg aaatttacag caaggcccgc gtggagatcc
    56521 gcggggcccg gaagggcatg gaatcgggag ggctgccatg aagagcatgt tccggtgggg
    56581 gctgggcctg gcgctggccg cggcggcccg ggtcgcggcg ggggcggagg cggcggcgcc
    56641 ggcgccggag gatccggcca cggtcatcac gtccgaccgc ctgacgttcg acgcgaagca
    56701 gcggatcgcg ctgttcgagt acaacgtcct ggtcacggac ccgaagatgc agctggcggc
    56761 ggacaagctg acggtgcagt tcgacgagaa gggccaggcg aagacgatca aggcggaggg
    56821 ccgggtgacc atcacgcagc tcgaccgcac ggcgcagtcg gagctggcga cgtacgacgt
    56881 ggcctccggc aagatcgtgc tctcgggcaa gccgcggatc acgcgcgggc gggacgtgct
    56941 ggagggggac gtgatcacct actggcggaa cgaggacaag atgatttgcc aacccggcgc
    57001 ccggctgcta atctatcctg aagagggcgg cgtgaaggat cagttcagag gagaataagg
    57061 gcgtggcggc gcacagcact ggactggtcg aggcccggca gatcgtcaag gcctaccgcg
    57121 gccgcaaggt cgtcaacggc gtggacctct ccgtgaaccg cggggagatc gtcggtctgc
    57181 tcggcccgaa cggcgcgggg aagacgacga cgttctacat gatcgtcggg ctg
//
LOCUS       DAOOTK010000008        55940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_458_length_55940_cov_6.644198, whole genome shotgun sequence.
ACCESSION   DAOOTK010000008 DAOOTK010000000
VERSION     DAOOTK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 55940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55940
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_458_length_55940_cov_6.644198"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK634_02060"
     CDS             <1..330
                     /locus_tag="PK634_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW96552.1"
                     /translation="QVLSDSGHAVEELDSGSAFKERWRKNGARPDLIIMDFSMPEYNG
                     LQLCEWLKGEGGAVPVILVSGFAATQPDIAKALRLKRTFFLQKPFSFREMTDVVTVAL
                     GETLIGE"
     gene            complement(432..941)
                     /locus_tag="PK634_02065"
     CDS             complement(432..941)
                     /locus_tag="PK634_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96553.1"
                     /translation="MKKAIGVLLALLFVSVFTLPAAAQESRLGGGVNYWRAIDDVDED
                     FDEDGISWLVSYQYCPSEMFRIEADLEIFPDGVFGRGDTDYAPQAFAILGSWIYAGVG
                     AGIYYFGDPIKDWADDPFYVLRAGLDLEVLPDALHLDIYGRYQFSDWGDIDVDTDTAI
                     LGGCVRFEF"
     gene            1100..4060
                     /locus_tag="PK634_02070"
     CDS             1100..4060
                     /locus_tag="PK634_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96554.1"
                     /translation="MKKTPVLLLAVLALAVFTLPAPADPPNTAILDGRPTEYDGADLG
                     GTFFGAASWGASDVITNLYVTWDAEYLYIALQGWESGNKFAVLLDADPGAGTGATTTT
                     NWVKNAMPYIGYNDVGWQASAAPGAENFGLDFMIASEGYYNNIVRVTYDGAGAPDSNN
                     VEAVFDQGNGVSPRGTPVDMVVQADSTACPLKGFEARIPWSVLYATNRLGEVQPGEVV
                     PQGAQVRVFASLHNNDTNSAYSSPDAIPAQTNAAAGGGWAAGLLTTANYVTYDVDADH
                     DGFPDLAPGDVNAPFLRFVAGVQSNHVVYARFNENVTTNTATNTANWAVNGESPGSVE
                     MLSDATFLLRLTNALPAAGMLVPVAADFIEDASTNSKHTFLCLFPTNAGISTSVTVRF
                     VLQTASGLGRTPGASNFFINGSTEPLEWGYPPATSMPLALIGGPLTSYYRDVTFPPGT
                     GSRVYYKYSGILKNTGTNNFEAIRLQKYADASRWLTLPTDGSSLVVTDYLGVAAHPWR
                     DPNDTNFDFYAMVYTNATRGDAGVRERVTMTFRLDLSGRDLYGVQRVLVVGSDPLRGF
                     NRNGTGIGDWPGNSYVGWDVGGIELVDDGTLGDATAADGVYSRTWSFTTNGLDPAMVG
                     DTPYSLIGGDSYTVPYYGGAWANRRTPRSFAYKYAVVFGAESAVLDPASDVEHYIETG
                     STNIVLDPYVWANSELPFAPAANPPQLVAMQPVAGGTVVLFTNNIGEAQHGVQFSTNL
                     AQGWTDYGQTAAGSGGNWTVTVENASNPMFFRLYAGAHPFRGVWWEPNPLPETGGTMR
                     IYYTQYSRGLAGDRNVQIAGNFPPSSWGPVPMTFMGDGTWYYDLAVDTNVIVSPNFEF
                     KPRNVSGTVWEGMGGGGDNYRAYRGTLRATWTPHSPTNGGTITITYDQAGGPLAAATN
                     INAHLGFDETWNNLTTNVMANIGGTTWQATIPVPTGATLSVNWVFNNKYSGAVTNWDS
                     PGYGGRDWRAFINK"
     gene            4073..4786
                     /locus_tag="PK634_02075"
     CDS             4073..4786
                     /locus_tag="PK634_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOW96555.1"
                     /translation="MTKQHRAFTLIELLVVIAIISILMSILIPVAKLGIENGRRATCR
                     SNLRQIGAALYLYAGDPVMNGEFTLIAAREGAELSNQYPLPPHAMLLGGVMPTKGVDA
                     GFVSNRVREILIDPKVWICPSDKVDGDNLEPVTPAVTIADIGQRNISYMYIAGHRLIS
                     SPENAAIAPMMCDEANAMERGDATPANMPKITDADNHGAEYRNVLYLDGHVAAIQNAD
                     AANSIFTNLVNTDILQSID"
     gene            complement(4801..5535)
                     /locus_tag="PK634_02080"
     CDS             complement(4801..5535)
                     /locus_tag="PK634_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOW96556.1"
                     /translation="MTPPPAFSRNELLLGLEAAEKLTDALVVIVGLGAVGSFALEALA
                     RAGVGRFRLADFDKVRPCNLNRLLTATHSTLGRKKVEAAAARVAEINPDGRVEALDLF
                     ADETSYPRILAGPPDVLIDAIDSVGPKAALLTAAVNAGARRVISCMGAANRGDPLAVK
                     VAMLSKTRDCPLAREVRGRVPKGLQQRILAVYSDEAPWNPPAPPEPDGALRRGRPRRT
                     LGSLCCVTGVFGLVAAREAIRAITDR"
     gene            complement(5532..7316)
                     /locus_tag="PK634_02085"
     CDS             complement(5532..7316)
                     /locus_tag="PK634_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOW96557.1"
                     /translation="MIRDLSIDVSPADAGPRLDRFLADRFPSTSRSLVARAIEQNAIL
                     VNDRPAAKGRRLAAGDRVRVVELLEQADWKAAPNPALPLAVIHEDPAFLVLDKPAGLP
                     VHPLEPGDTRTVVSALIARHPELSAIGPDPLFPAIAHRLDTDTSGLLLAARDESAYRS
                     FRRQFQAGDVDKRYIALVGGAIGTGGRLDQLLAHAGGGAHRMVVVPDGARGRRPMRAI
                     TEYEPREALPGFTRLDVRIRTGVTHQIRCQLASLGHPVAGDALYGSASPGIPRLFLHA
                     AEIAFRHPRAGTECRFHSPLPADLRAALESLRSRGSSTLHEKVPGGRSSRDAALSWID
                     AHCHLQDDRLAPDLDGVLARARAAGVERMVCCGSAESDWNRVVELAGRHPDIIPAFGL
                     HPWYLRERSAEWLPRLERLLIEHPRAGVGEIGLDHALPDRNDAEQEEVFLAQLHLARR
                     LGRPVWIHSRRAWGRMPELLRQFGRHEPGLVLHSYSGPAELMEKLAALNAYFSFSGTI
                     TRSNNRRGREAAARAPADRLLIETDAPDLPPVIRTPDGSAFVPPLNEPAHLVHVAAAL
                     AELRGWTLEETARVTGKNADRILQSLNP"
     gene            complement(7313..8212)
                     /locus_tag="PK634_02090"
     CDS             complement(7313..8212)
                     /locus_tag="PK634_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96558.1"
                     /translation="MKHRLRDYVEYGLVRGLTAIVGRLPHRAALGLGWVLARLVFLVA
                     RRRMHRFERRIAQVFGDAKSPRERRRILWLAWRNLVFNGIESMRTPLVTREWISRVVD
                     QRDVHLLFENMKEGRGAVLAVPHMGNWELAGVGMQLLGARLMIIVRRQRNMLTYEWLN
                     RVRASAGLESFLREAHSFTGIVKGLQHGKVLAILPDLRAKAQFVAAPFLGHTAQIPAG
                     MAAFAREAGVPVIPAYAVREGWTRHRWVGFPPIHPDPAFDRDADIERMTRYVIGFFDQ
                     AIRAHPDQYYWFNGRWVLGEEPP"
     gene            complement(8209..8961)
                     /locus_tag="PK634_02095"
     CDS             complement(8209..8961)
                     /locus_tag="PK634_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOW96559.1"
                     /translation="MRIRKPAITMGFALGLLGAAGGAPAQAGDAAVAPPLAAVPAGVF
                     VMGGSEAQPPRELRRIDMPAFRLGQYEVTVEEWAACLNGGGQPDYPGSPQVTRRGKRF
                     RPARGQGRNPAAWISLADAQAYCRWLSERAGLRARLPTEAEWEYAARGGLEGARFPWG
                     WGAPEGRACFNARAPRRVGAFPPNGYGLCDMAGNVFEWCAPDNAGGPPVARGGSWSER
                     DPRYLRVYNRVPFPAAYRNADVGFRILVEGAE"
     gene            9098..10471
                     /locus_tag="PK634_02100"
     CDS             9098..10471
                     /locus_tag="PK634_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96560.1"
                     /translation="MKCDAARRNGSGMQLLGLLPLIALGGLLGYLLAGDVFSASVRKL
                     AASLSLGLVWAYLFLQWIDLVAEYWALKRDPVPADLDVADRSAVASRLESLCGGSAVT
                     TRVRHLVKSWSLGWHPRQVMGLAGFQSAQAKGQLYGGVVFALILLGVAVWLQGNSLWF
                     WAAMLLLGLTVLARQTLQLRVDRYIECRLLACLPGNIPQTAMTAADLAGALGGAIQSA
                     FKNHVPQPDAMAAAMRTAMDGAMQQVAGAIEKLQKSLGEGQGAVVEKWTQAVHGAASD
                     LKGLQEALKAATGDLKSGLASASGDLKGSLATGAEQWKSVLQGHAQSLAAGTEQWKGV
                     LQNHAQSLDKSLAAVPGQLAKTYGEGAGQVVAAFKDHAEKMAAASQGIQTQLDRIAQL
                     GKEIEKVLHVQQAVDGTLKAVAATEEFRKTLDTLNKHVEASDKLLREAARPKTIRLVE
                     SDDDAKG"
     gene            complement(10540..10959)
                     /locus_tag="PK634_02105"
     CDS             complement(10540..10959)
                     /locus_tag="PK634_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96561.1"
                     /translation="MIPDLQSSILCDDVRQERNGKFILIGLFDAIGAPKFPLVYPRLF
                     MVTRWCSGEGVFKQRTRVLRPDGLSVLAEGKDIPVRLPNAEATATNIEAFMNVVFQEE
                     GTHWIEILLEGDLKVRFPLRVNKIRPPPAGVPPAEEA"
     gene            complement(11039..13129)
                     /gene="fusA"
                     /locus_tag="PK634_02110"
     CDS             complement(11039..13129)
                     /gene="fusA"
                     /locus_tag="PK634_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOW96562.1"
                     /translation="MPRQVQLSNIRNIGIMAHIDAGKTTVSERILFFTGKTHKLGEVH
                     DGAAQLDWMPQERERGITITSAATTTLWRDHRITLIDTPGHVDFTVEVERSLRVLDGA
                     IALFCAVGGVEPQAEKVWHQSEKYNVPKIAFVNKMDRVGADFFAVLEEIQNDLGANAV
                     PVVVPIGKEEAFQGIIDLVSMKALYYDGDNQGVTFREESIPADKMEKARKWRANLVEK
                     CAEQDDALLEKFVEKGDLTKDEILGILRKATIARRVVPVYCGSAFKNKGIQRLLDGVI
                     RCLPAPNEIPPIICAREPEANKREPTDEDPFSALAFKVVSDKHMGKLTYIRIYSGRLE
                     SGCTVLNSSRDKTQRISRLFRMHANRQEVITEAFTGDIVAVVGLSDTRTGDTLCTEAQ
                     PIHLEAIEFPAPVVSISIAPETQGDSERLGEALHRLADEDPTFTVAFDAETSETIISG
                     MGELHLEIIVDRLKREFNVAAKVGRPEVAYRETATVPVEGEGKYIKQSGGRGQYGHVC
                     MKMEPLPPGDGFEFVNDVVGGRIPLQYIPSVEKGVVRALRSGPYAGYPVVDLRVSLYD
                     GSYHEVDSNDFAFQEAARIAFKQMFLKGQPELLEPVMSVEVVAPEEFMGPCASSICQR
                     RGRIESMTRKGDLQIIEGLVPLAEMFGYANALRTLTQGRASFSMHFEHYEAVPDALAE
                     TIVEKRRKEKKVRG"
     gene            13351..16881
                     /locus_tag="PK634_02115"
     CDS             13351..16881
                     /locus_tag="PK634_02115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOW96563.1"
                     /translation="MNLKTVPFVHLHFHTSYSLLDGACQPDRVMERAQELGMTAVAIT
                     DHGVLYGIVDFYKQARSRGIKPVLGCEVYVASDGRRERNTNAPRHHLVLLAENLQGWH
                     NLVRLVSLAHLEGFYYKPRIDKELLAQHHEGLIGLASCLHGEVAAACAHEDPAAAERA
                     ARSYTEILGPGNFFLEIQDHGLPEQKIANRGILEVARRTGLPVVATNDVHYLRPEHAE
                     AHEVLLCLQTQTVMTDPKRMRYASDQFFMKSGEEMQRLFPDHPEVLANTVEIAERCNV
                     ELRLERDLHFPRYEIPGGKDEKSYLEQLGREGLRRRYGIEDLDHPKNEQEKKIRDRFH
                     FELGVIEKTRFLNYFLVVWDFVRFAHEQHIPVGPGRGSGAGSIVAYALGITGIDPLRY
                     NLIFERFLNPERISPPDFDIDFCQARRGEVIDYVREKYGRDNVAQIITFGSLGAKTVI
                     RDIGRVLEIPLGECDRLAKMVPDDPKMTLEIALKQNPEFRKARDTEDHARHILKYAEV
                     LEGLPRNPGTHAAGVVIGEKPLIELVPLQRDKDGEVITQFEMEPLGEIGLLKMDFLGL
                     KTLTVIQEAVDLVRAGRGVELDMANLPMDDAATFDLLNRGDTVGVFQVESKGMRDLLR
                     RIGLTRFEDLIAMIALFRPGPMNMLDDYVNRKHGKVKLTYDHPLMEPILRETYGVMLY
                     QEQVQQVANVLAGFSLGQGDILRRAMGKKKKEVMDEMEEKFVQGCQKTNHVPPRVAKK
                     VFEDISRFAGYGFNKSHSAAYAVLSYQTAWLKVHYPVEFMAALLSSEIGNTDKLPLLV
                     AEGKEMGIEILPPNVNDSDVRFKPVGDAIRFGMAGVKNVGTGAVAAIVAERTARGPFK
                     GLVDFCGRVDAQLVNKKTLESLVKCGAFDFSGMSRGRLFAGLDFAVSHAAAMQRDKRS
                     GQTSLFDLLAPTEGTPVQDELPPGEPWPESQMLAAERELLGFYISGHPLTAYEWALRT
                     FGVANMAGLEAVPSGGAARVGGLVAQFQKKFTKKEEKPMGVFRLELLDGAVEAVAFPE
                     AFREYGVHLQDAAPVMLCGVVKKGDQTKIEVQEIYPLKDVHRMFAERLSVHVSAAGLD
                     DAKLWKIKELLARHPGPTPVTLCLQFPTGEKVFVKADRTFHVEAAEALVRELEHVVGE
                     EGVYVEVVKRACRKKNGARNGRGRWGGRPE"
     gene            complement(16898..18826)
                     /locus_tag="PK634_02120"
     CDS             complement(16898..18826)
                     /locus_tag="PK634_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96564.1"
                     /translation="MRPLFPSLAGAFGLIALCAGLCGGCGGAPGEREFDRGMAEFQRE
                     RWVRARALLEKSIARRPGHADNAVAYHYLGVACWRMGQVQPALEAFEACQRLKPGMAE
                     SLYNTGVIFFEGGDPARAEPLFQEAALARPGDARPLEFIGAIYAQAGQWAEARRVLSV
                     AQARAPQSARILNARACVEARAGKPDQAAFYLMQSLEKDAGYAPALFNLARLHEGQDA
                     KQAESYFEKYIESVRNDPVARTGPQAEYAEHALGDLAAEKPTPAPAPPPAPAAKSFEA
                     LLKEAADEASRGHVSSALHLCLEASGRAERQRDTGKQEKALREAVRLAPQQARAHYAL
                     GRFLAGQDKADAALDCFKTAVELDPRFSLAQLGLAEAAVKAGEFDAALVACRAVVQQE
                     PDNPEALWLQATLYDDPLDMPEPAARTYREFERRFPGDPRVVRARERLKILQPPRTEP
                     PRRPPAEPAPAPGPEAPAPTAADADGARHVPFKPPATPNLQAAVQAYNRGTTYQQQGD
                     LDRAIFYYLRAVENDDSFATAFFNLGIVYTSRRDFDLAKDAYRLALERQPDLVAARYN
                     LAVLLTEQGEPEAAAEQYTLLLKAHPDHAASHYALGLLYAGKAGTYGLARRHYEAFLR
                     LEPEGPAAGAVRDWLSIH"
     gene            complement(18894..19124)
                     /locus_tag="PK634_02125"
     CDS             complement(18894..19124)
                     /locus_tag="PK634_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96565.1"
                     /translation="MMSGLSFFIYGKGASSDIPYRDLWGGLYDGTHHAPRESRFLLTG
                     ILGIGLLLWGAALLWRRRHGTGRWSAQSGPAG"
     gene            complement(19561..19947)
                     /locus_tag="PK634_02130"
     CDS             complement(19561..19947)
                     /locus_tag="PK634_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOW96566.1"
                     /translation="MQSFSVSTRQRTEFVDITRKVESAVEAMGARDGVVTVFVPHTTA
                     GVTVNEHADPDVMADMDAALERVVPWRAGYAHAEGNAAAHVKASLMGSSAQVIIAEGR
                     LRLGTWQGLFLCEFDGPRTRTVWVAR"
     gene            20287..20703
                     /locus_tag="PK634_02135"
     CDS             20287..20703
                     /locus_tag="PK634_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96567.1"
                     /translation="MKLIACAGIVVITLGLVSCGGGKYADAKAAMNDQIAFMDEFVSQ
                     VDKASSAKDVAAAVNRYSEQMTRMLEQSTKLKEKYKGVDLENAPELKEEMEKVKASAM
                     KFAQAFMKIGTQYGNDPEVQTATAKWRETMTKAAQP"
     gene            21131..21379
                     /locus_tag="PK634_02140"
     CDS             21131..21379
                     /locus_tag="PK634_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOW96568.1"
                     /translation="MATPESTRRPAQRILQVFVHRLANAASEGLNNKIQTLIKKACGY
                     RNRSRFKTDIFFDCGGLDLYPPFQPVKSPANMSEELLL"
     gene            21560..30301
                     /locus_tag="PK634_02145"
     CDS             21560..30301
                     /locus_tag="PK634_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOW96569.1"
                     /translation="MSRFSRRISFSNHWKKSAGIFQSLENTRVLFPIIGIGLLGLAAT
                     ASAQTDFEKVTNAVHTVRLELEAALGKPVPSLSLLVQTPADTFFTCSAATPEQAVATN
                     TTFRFASNTKNFTATAVLHMQQQGWLDISDPITNTIPGFSEPYIPTGTNWAIPNKPII
                     TIEQLLRHSAGVYDVDNDDVPGCGGTSYTDYMITLDPAHQFTVEEMAGQAALHQLSYS
                     APDEGYHYSNTGYAMLAEIVGRIYSLHSGSTKKLTDYLYDHIIGGASPVPIPEIHFPN
                     LATDTALPEPFMWGTERRPTNITIIVSNCNMSAQVGEGNGYGTLPALNRFIRATMKGE
                     GVLSHESVRLMQTDTSPLNAGYALGCTFVTDLGFGHNGCRVGNLSHMNYNPETDVSVA
                     VYLPLVDYSDNMDSFTLCFQAIYNAAYRALEALGYPRTPTLAHGSQTNLTLSAGATNT
                     FYFTAAQGAFYGVKISNTVADIFCRVAPGVDPDDAVTVTNNFTWQGHAPGTYRLDVSS
                     TAATECALSLIALKMPDPSDFSALNWTNAFIAAHDVFSRQYAFGDWKSVDWGALYSNT
                     LPRIAAAQALTNEVAYYAALHEYVGSVPDGHVALKGTNAAVPAAWGEEVAGGGYGLAV
                     AELDDGRVIAAALLTHGPAQLAGMEAGAEIVSWDGLAPASAMAQINAAAYPLRALAGP
                     MTSCPQATLAQQRLEQARLLVRGPVGTTVDVQFINPGSLATQAVALTATADTNATFAL
                     LDFAMRQNPSSDPVEYRLMTNGLGYLLIRFEANFAEPENLYVINSNVAEAVKFFVASN
                     APGVIVDVRGNLGGDDQLAATLCGYFHRTNMFYERQTWYNTLNGTFTELTFDSMNGWA
                     PLDYMAIPTQSVFYGGPVAVLVNLGTVSSGEGVAMGIGALPNARVIGFHGTHGSFGMA
                     GATIALPGGYSIHYPFGQSLDENGVIQLDSRNGVGGVPPEIRIPKSAENVLAFAAGTD
                     VELNYASDYLIGLRNYTNTIAQTTALITNLMAQHDIPALSIALVDGANIVWAQGFGTA
                     NREAGTPASADTIYRIASCSKTFAAASIMRLQDDGLLTLQDDVTNTVPAFSLLPRYPD
                     QALTIRELLAHQAQLPGCYFRHADSTIPYDGYYDMSLALMVEDYLVEPRHFLQKYNNN
                     GFTLIEGVVAGANTNGLDYAAFAITNLFQPMGMSNTTFRLADHQTNLLAKVYRSGVLA
                     PDEFVNTPGSGGAYSTAADMARYARFLLGYGTFNGTRILSSNAVAALWADQSAQVALV
                     NKEDYAKSGLGWDQIADPSFAYAGRMAWKDGDSLNYSAIICVMPEHGLAAVALNSIPG
                     ADTAAAPAAKLALKLALQERDGIPLPSTNPPAFPVSPVTVLTEQELAGIIGTYSAAGG
                     YDLVTGTVSAITWITGADGSSPVRYEGLVPHQDGWFSATNAPEFRLSFTNIGGRVILR
                     SHVRYEEYLRIIAQAERFTPAPLSAAWSNRVGKTWITADLEPESYLWLLGFYPTLELG
                     MADGVLSVYSSPCQTLQPQGDDLAFPFSMNAAQPSALQVKHTNGHEVIRYSGYDFRRV
                     DDLPVLQPGQSATGLLAAGASAPFQFSIADPVWHGIHISPSNLILRQATADLSAPGTY
                     SLILQNPGTSAAPYSLRLYNLTSTIARVSTLITNLMATHQLVGCGISLVDGDQTVWQT
                     GFGYADRERGRAADARTPFMIGSISKTFGALAAMQLAEEGLLDLHASITNALPSFAIH
                     QRFADNTITPHTILTHHSGLPGDVFNQGFTVRPLPGVPDAVQAMLANEYALMPTNTMW
                     AYNNSGFVMLGQMFRHLTGQELDVFARARLFDRMGMTNSSIAFDLPAIRQTRARPYVG
                     GQIGPDEYVNLYFAGAIYSTAADMARYMRMLLAGGMGENARVVSNQTLQAMATRQNAG
                     IALDQYASMLNMGIGFVLDPPNLRYMGKVLWHDGGTVYFRSLLRVATEAQLGCFISCN
                     SYEGGAMTYTVVDSALQWAYEEKTGLAPPAPLDPGAPGGAVAPPEVITLATGGVFVTS
                     GGYDTFRYDGTNLFARFNAQLDESPESAWTYRENGWFTPTNAYAPQLEFVQVEGRILS
                     VIQTFDYAVTNLILLGERAAGLAPFHPAWSNRLGRWWATDLHPDDIAWHGDDLRLAWP
                     ALQLTARDNMLLVQVEDLYVMSATNDALAFAAGLGRNKGSSLRAAGPDAIAYMGVHYR
                     AEAGIPQLAPGASTNGSTLGDEIQWFQIPAAAASLTFDLATEHDLAAYLYTEDDEYLG
                     QANRSHALHLDATNAQPVMAAILRNGTNTGPWTLATYTNAIPFYSQVPADEWPAALAA
                     HSNLFPNTDFGHVFARENRERGPGVSPVLKIALARMKSPNPSPRPLFFLNGGPGDSGI
                     RCAYQYFLKAFTGTHDVYLIDPRGVSYSQPSLDFFADEDLPEWQYRLTMLQQADLSAL
                     NTLESSHDVDDIAAAFGVAEADLIGQSYGTLLAQTVMRREPAWLRSVILDGVVAPNIP
                     GLSQAGPVRNEALENLFADVAAHPQASVWYPAFGLALHTLSTNLQNNPVDLVISGETN
                     QMNGLSFLDAVFNQLTSTDLAARERIPNIVWRATRGETAALAELLTEFRKDTNLFMTT
                     CKNMEQQILVIKHDFLPFDSLEAASNACANLPPLVARTALDFMTDAIEGAALFDDYGQ
                     ADNAFTNPVTVSIPTLVINGTYDTQTGTNWAAEVARHLPNAHYVAPPAVGHGVLFATN
                     GCMTQLFRDFLADPTNPPDTSCLAGLVPDFPPPWPANTRDVSVGQTFTNAFEAPDSAQ
                     WNRFTAVSGVVYTVTQTGGDALRVVDENANTLASVQNEAVSWESSGDGRIYLMAIASR
                     AGTNSITLDLPLLFRHLAFNGQRATLNWQSLTNLSVNLWMITNLGGNAEPICVHSNIA
                     AQGWFTTLDHEDETAHPSAFYFLKDTE"
     gene            30645..31415
                     /locus_tag="PK634_02150"
     CDS             30645..31415
                     /locus_tag="PK634_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOW96570.1"
                     /translation="MIEVVAAASRLDADGIMARLDPDPALRYYVQGQSFNYPDLAVFL
                     RQSFADMTTQRVVWLESSARELAPDTVLWTSYGRNPVVEASGKEIEYILGETWVWKKT
                     DGNWRAIHYHESFLEAPSREKRERVESALSEFAASLKIESRNPEPVMAVLNEFVRSHP
                     EVVGSAFAFAPTEEGSWPAPYVHRKGSEIIRKTLPSDSGYERSDWYAVPAASGQPAWS
                     EPYFDTHGAQTMMITYSIPITDTTGDLLGVLTADVAFY"
     gene            31427..32632
                     /locus_tag="PK634_02155"
     CDS             31427..32632
                     /locus_tag="PK634_02155"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOW96571.1"
                     /translation="MTTSNPDDRTRILRTVRGQEVTPDRDVTAACGVEAKNLKRAVFP
                     GKYIQGRGALGELPLLMDSLGGKGLVLASPSAHKNILPACGIDWGSRNTILDSFQGEC
                     CERELSRVAELIGRHQVDILVGMGGGKAIDTAKIAADRAGIPVIIVPTIASTDAPCSG
                     CAVLYSDDHVFESVYYQKMNPAAVLVDERIIVQAPVRFFIAGMGDALATWFEARSCST
                     TQSPNACGGLSPMAGLHIARLCYETLIGYGVAAKLACEQKAITPAFSRVVEANILLSG
                     IGFESGGLGAAHSIHNGFTALEESHAFYHGEKVAFGVLAGLQLADAPPAESEEVFSFS
                     EKIGLPTTLADLGLGKRGRADLMKVAEKACDPVECIHHEAGVITPDKVLDAILAADAI
                     GRSRKGSSG"
     gene            32691..35324
                     /locus_tag="PK634_02160"
     CDS             32691..35324
                     /locus_tag="PK634_02160"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOW96572.1"
                     /translation="MAIAVLAGLSGVWTARAQIPFTNTIAASRATITNVMAEYGIPGC
                     TVVLVESQRVVWAEGFGLADLETQAPVTTNTVMMIGSVSKFFTAIMAAQLADEGALDL
                     NLSVTNYLPDFSMRDRFAGPPEAWTTRNLLDHHAGLPGDIYNGSFGASDYWPGYIAWM
                     IHYFQRDYPLYPPNVVASYCNSGFNLVGEIVTRHDGVDYAVAAENRIFAPLDMPYSSF
                     LPDKPLVAENLATGHSAGQPTPPMIGNMAATGGAFSRPLDMARIITMILADGVAGGSN
                     YLSQAILDEMGTPANAPLDLDIFLQPGLGLDSVADHALAYAGNVWSKSGDTGTFSAML
                     QVLRERQLGVFVNINMSHSAGFTISRAILREALLERDNLPDPGWPPMPDPAATNWPYA
                     ALQDIEGYYITAAGVDRFIAEADGTLTCIHDAQNNTEATTSWYPHVHGRFYPPGLHHE
                     QFVFTNRAGCDLILRYGSSGSARDEIVYGGYAEGLYGVRYTPPAISAAWSNRVGTKWL
                     ACNLWHDDWMYEAGALPAFLLTAGHGILSLAGPQGAVLEPVDDGLAFVGGLLTRGDSA
                     VRIETNALGREFLWFGGYRCERYDDLPELQKGVAVSGAASAHTNALFRYEHGAEGQSI
                     LLALTENASKAVLTVFNADIEPIGRGEGCLALDLSSDAPLIVSITSADPVEFDIKTVD
                     ITEVRAAMRRTLDQYPFVPGFGVAAQEPGFPPILLTEGHARISPPSPEPSLPLQGHEQ
                     FHIASISKTYTAAAIFLLQQRGLLNIADCVTNYAPELNIPRADEITLEMLLQHRSGLP
                     DANNTGWFDGKLENNPLLEFTVEDIVDVAHGLYPNLLFEPGTAYHYTDTGYNILARVI
                     ENVTARITKASWPTMSCGRSD"
     gene            35360..38284
                     /locus_tag="PK634_02165"
     CDS             35360..38284
                     /locus_tag="PK634_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOW96573.1"
                     /translation="MPEPSISTYMLIKGEFQDRSIWNPSVEFGCGSMITTLEELMDMA
                     HAFFMTTNLLDETTHALMMERVSATGLDHYGRGCNYAEGLGWGHDGTMWGALSTARVD
                     TNNGVRLAAVMSGQYEDERLMGSFLAMRSALGLLKNALGYDAGTVGRHEPVVYPVLGA
                     ARQGHPFRFQPLSFNFPTNWAVTGLPDGLTFDPATGEITGATPIRGTHALTVTAQNAY
                     GSVEVELELEVQTGYTNTIAAVSNHIVAAMVEEGTIGVSIALVDDQEIVWAQGFGFAD
                     REAGLPVTTDTVFHIGSVSKAFTAALNLQYAERGLLDLDAPFTNYTPDITWMDRYPAA
                     RAITVKDLLTHHSGLPGDLIRAGFLTQPLGRGYLETTHDLAQTYPVLEPGTINNYCNV
                     GFVLLEGVAEAAAATEGDPRSFAELANARLFEILDMDATSFKFDKPAISNHLAVPYMG
                     GERMPPEYVEIYGTGSIYSRPVDMAQFMKALFSDTPLVLRPETRDLMIADHSTNAPFD
                     AFEWVKTGLGWDTVSDPRLSYAGPAVWKNGGTLAYSAQFLFLPEKKLGVAIAASSPSG
                     IPMAVDALALQHALLERDGLPIPTNAIAWPTNLAAVSQAELDALAGVYVGSANYDLVE
                     SQPGFLSYRRNVPDDGLSLQNLRLRSDGWFRSDDKPDHAISFTNAHGRNLVLHRQNHG
                     FYDTPSILAERFAPPLLPAAWSNRLGRTWIARNAPPDDYIRLLGVAPTLQFIFEDNLL
                     RVNPGGILADRVLEPDSDDLAWIPGLVNRGDSAVQILHLGGVEHVLYAGFVFGPEPAD
                     IPLAGTASNTIAAERFADWYAIQPAGPPAPVGGVDDIQYQLTLSDAPNTFLLRVYEAD
                     GVTPVAERTGNGTLDFSSGNTPLLLNLQPGSDGPPTGAYQIDFSVPLLVRALAVHSST
                     VDMVWQGPANTPVTVEAAAHLDATNTFAVRTNLPPDASLLRQQILSPDSASLQFFRLS
                     SP"
     gene            38430..38672
                     /locus_tag="PK634_02170"
     CDS             38430..38672
                     /locus_tag="PK634_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOW96574.1"
                     /translation="MRINMAGGWRHRPSRGQKREPIYDRDRQEFLDRLEEMTKRYGVG
                     AHAYVLRPNHYHLLIRTPKANASAAMQRLNNGYGMW"
     gene            complement(39154..>40702)
                     /locus_tag="PK634_02175"
     CDS             complement(39154..>40702)
                     /locus_tag="PK634_02175"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HOW96575.1"
                     /translation="VTATTMPAGLTVEFTYDGNAWAPTNVGSYAVTGTVNDINYQGSV
                     ADTLVVSKGVAGVFLQNLAQAYDGTAKSVTATSDPAGLVVEFTYDGNAWAPTNAGSYA
                     VTGTINDVNYEGSAADTLVVSKADQIITDFLPTNGSVFVEDAAVGLSASASSGLPVSF
                     AVGGDPGVINDGTNLTFTGPGEAIIVASQAGAANWNPAPDVTNHYTVLAIVTVDIESP
                     YGITVPTTGRHAYVEGTVITNQAQSPDTRGTTQYVCAGWVATEGLAPDHGDGTQAVVT
                     VINSATLTWLWRTEYRLESGAGPNGSVDRLGGWFTNNEPVSIEALPDPYFVFTNWTGD
                     VAGPDEFTNPLNLVMDGAKSVTAWFTALWTTNRPTPHWWLAQFGITNDFEDAVNDDPD
                     GDAIPTGDEFVMNTDPTNGLSFLHVARISEVYGTNCYDVVWTNSESPFEEVTQTFCDV
                     IGHLLSWPAATGRVYDVEFDTDYPWQNWAPVEGLTNLTTETGWISMTNVLDATALKLY
                     RLRVHLP"
     assembly_gap    40703..40802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40803..48572)
                     /locus_tag="PK634_02180"
     CDS             complement(<40803..48572)
                     /locus_tag="PK634_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HOW96576.1"
                     /translation="MSIGWACGGLFAVLYLAVAVSALGQVLTTNISPTNLPAGHGAVL
                     WQDFETAWTASNTPAGGWPTTRGWSKLIMSNNTDWVRATNGINGHPPRAREGAWSNPG
                     TGTFSNFARYGAASYGNVTRLYTPPVDLTGFQGTPTMAFWHCQVPWPADQDYLYVLVH
                     TNITPETWSNRGARAGAGGWQVLAYYTAGLTNWTQHSLAITNVLGTNMIFAFEGIGEY
                     AYGTCVDDMIVFGNNTAAPVFGQVTNLWQDFETAWTASNTPAGGWPTTRGWTKLIMSN
                     NTDWVRATNGINGHPPRAREGAWTNPVTGTFSNFARYGAASYGNVTRLYTPPLDLSGA
                     QYMPTMTFWHCQVPWPADQDYLYVLAHTNITPEAWTNKGGRAGAGGWQVLAYYTAGVT
                     NWTQRSLTITNVLGTNMVFAFEGIGEYAYGVCVDDLQIISMILDDTPESTNGGIALSP
                     SSLAFAGVYGGASPAGQAFAVSNVGSTAFTWTGTVSAAWLSCVPPGGTVDTGSSTEVT
                     ASVDSSGMAAGVYTANLAIASANATNSPQTLSVTVTVSKAAQTITFPAIGWQHVTNAV
                     GLAATGGGSGEPVTFAVTDGPGAIAEGTNLTFSGAGDVIVVASQAGNANYEAAAEATN
                     IVSVYALSASAGAFAGGNTITISNGYFGTITNVLVGGVAATIKASGAKLVGGAAATIK
                     ASGANWVTITVPFIGTAGVKDIVIQTSDHGDTTLAAAYTVNAAGEIGSSVSVKRWSAV
                     GKELVPGQTNAIGANNTIYGMNYYGNNLYISGAFTNAGGSNCARVARYDGTNWNSMGI
                     GVPKAANVNCIKGGAYGVYAGGYFTNIGGIGGLAVGRWDGTNWNPMGRPPAAGDTNRM
                     GLGFTPTINGYVNWMEPYKSNTVVAVGYFTNTDGRVGSVNYVAQYDGVGWTNMQGGFR
                     NVPLTAAYDRDRDHLYVGGSFTNHYPTNESVHLNFIAQWNGTRWTNMARGLGNRATCM
                     ALHPAGGELYIGGWFTNYTDSAGNKQLANYVVKWDPVSQSLTNVGSGFNNWVYALAVD
                     TNGTLYAAGAFTNTYLSTEPDKSAPKLATKRIAMWNGTHWTNMGCGLSDTALALTVNT
                     NNSDLCAGGFFRIAYQDDGAGTDTWYVAKYGTEDVASSGVEPSSGSVTGGYEVVITGS
                     NLGSGSDITNVTLCGVSGASMVSQSATQVVVVAGVSWAAGLGDVRVYSESYGETVKSN
                     AFTYNGAFLGLRGTNGAEIASDEAASESKGTDFGLVLPGGAVTNTLAITNGGNETLTL
                     TGWTTNGSEGFLVVSGPASVNAGDTADFVVAFAPSAGGTATVAVHIAHDGGGDNPFVL
                     NLAGQSGKQDQTITFPPIGAQPTNAVAGLAATASSGLAVSFVTNGGPGVISDGTNLSF
                     TGAGEVLVVASQAGDATWNPAPEVTNTLHVYALDMWTGPYAGGNTITITNGHFGTITN
                     VLVGGVAASVQASGSDWVTIVVPATGSAGVKDVVIQTDGGDITLAGAYMVNPAGLIGR
                     PGSLTGWVEVVGLPYGQAAFGAGVVSNKLYVAGGRNASSTVTNVTCFDGATWSEVAGL
                     PQPRVDCRAAELNGNLYVVGGSYNSSARTNVYMYSGGVWSEVAGLPADRTAIALAYWR
                     NALYVMGGEVGGTACTNVYRFDGVSWTETKGLPSTRSYNTAAVLDDQLYSIGGKQAGT
                     TYTNVYRFDGTNWTETAGLPQSLCSMVTYTVGGFIYAISGWTGSNASSNTFRYDGETW
                     MEVVSLPQARSSAGCGALGTNMYVVGGDTGSTVHTNVWKGLGGYSGGVLPTSGSSGDV
                     VTIYGIHLGNGGDITNVTLCGVSAAIQSQSATQIVVTAGATAGAILGDVRVFSTSQGE
                     TVKSNGFTYTASGIGISGPAFAPAALGAVVTNIFTVTNFGNEALLITAATNDGEGAAY
                     FNVSALVGLTVEPGTASNFPVVFTASNVGSFTPTCYVDNNSPLPSYAFGLTGAGFQAA
                     PNIGPFAGGNTIAIINGVFGTITNVTVGGVSAPFLPQAANVVLITAPALGTTGTVDIV
                     VQTEEHGDITFAGAYTYNPAGRIGSGQGVKVVDISSAPLTGWSIFLGDLMGDGYISNA
                     LAGDNVLVLNSDMMTQTLERWNWTQALLPQVVFTGTTVDNLDSGSDINGTLSWWNVDA
                     MSFNMIEWTDGIYGYFGIRFQFDGQADYRYGWVEASRNDTDTFSVRRLAFVSVDGQTL
                     QAGQTNLPGASGVLPESGSWTGGYEVVISGTNLCNGTDATNVTLCGMSAAIQSQSATQ
                     IVVIAGQAAAAGVGDVRVFSTSFGETVKSNAFEYLREHQASLVFNPASPQAYLTTNAL
                     TVSGGSGTGAASFEVLSGPGILVDDTNLAVTNSSGTIEIRATKAQDDLYFEASVTGAV
                     VASKAVAEVYLLDLTQTYDGAAKSVTATTMPAGLTVEFTYDGSATAPTAADSYAVTGT
                     VNDANYEGSASDTLTINKAGQTIADFLPTNGSVFVTTDAAGLSAAASSGLAVSFATNG
                     GPGVISDGTNMAFAGAGDVLVVASQAGDANWNPAAEVTNVYTVGKAAAQVYLLDLAQT
                     YDGAAKSVTATTMPAGLTVEFTYDGHAWAPTNAGSYAVTGTVNDANYEGSAS"
     gene            complement(49013..49303)
                     /locus_tag="PK634_02185"
     CDS             complement(49013..49303)
                     /locus_tag="PK634_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96577.1"
                     /translation="MVKADNERGPAPAREAVSRNVFDGILLDLELPNGNGLNWLPELR
                     DSHPDAAIVIAPPLSEKAISSAGAMWREKREGRLYDAEPLQNACRIETGVSY"
     gene            49435..50928
                     /locus_tag="PK634_02190"
     CDS             49435..50928
                     /locus_tag="PK634_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW96578.1"
                     /translation="MEPRAALTRPAARILWIARGVCLLALAGTLLGLLAWCRLKGWSH
                     DEVENIHAGWLVYKGQVPYADFFHHKPPFYWFFISLYYRLAGENLAVMLWARHWMLAT
                     FVLAALFTFLVARELFDEWAGWAAAGILVCNALIHYPAIEPREQGWMLAFLMMGLYVY
                     LRAWRRAFRPRDGLLAGLLLGAAYALHPRAGYPILALALGTGFRCQFQTGWRVVGERK
                     AALALLALAFLGVAAIPYILYGKEFHHLLYRVSLAVAPEDRFPPWGLLKNLYFSTYAI
                     MPFALLGLAACAADLAKKGERQWAAFCALVFFTLANLQIISNPRAFVQAFYSAGPFVA
                     LLCAAAVHWIGRRLTAAQTALGVACLVTGGFLSNVNPSYWTAPDEYAQAERRARYLLE
                     MIPPGERYLGMDAHPIFRLDGSRYWFDMNHVLRSLQLLDPTFRHDVVQELEQTRPYLL
                     GEDVFGKADFDPVQKPRLETYVSERYQRLPNAPFFIRKDALSPDRAP"
     gene            50925..52340
                     /locus_tag="PK634_02195"
     CDS             50925..52340
                     /locus_tag="PK634_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96579.1"
                     /translation="MKILVLNPPAHEGRPYIREGRCMQIGSSWATLWMPLTLAYVAAV
                     LREDGHDVRLADAVADRLDLSGVLALRDEFKPDLVFLNTAFPSIEGDRAAAAALKRRA
                     PGVPIVLLGLFPTLLEDKALDYIAGADFGLIGEPEWAARELAAALAEGGDRTGVKGLA
                     WRRGGQIVVNAPQDLRSHDLDALPFPARDLLHNDAYRLPINGERFTLLNIGRGCPYPC
                     IFCIAPVYYGAPFRKRIVSRIGDEIQECVERHGLRHFLFWGESFTTDAAYAEAICEEI
                     IRRGFRVVWSTTSRVDTLTPRLLGKMKQAGCALLGLGIESADETILAHSGKKTRREDI
                     TGAIRMVHDAGIKTMGHFIFGLPGETRETAGETIRFALRSGLTYAQFYCALPYPKTRL
                     GELAAERGWITSGDYSSYDFTFSILRNESLTAREVKAFRDRAYRRFYFRPRMLFRVLR
                     EVQSVRGLLASMDFLKWIRKK"
     gene            52370..54472
                     /locus_tag="PK634_02200"
     CDS             52370..54472
                     /locus_tag="PK634_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96580.1"
                     /translation="MSRKKKKALKTGAQEDAPAAPKVVPEPDGPKRMALGKTGDATSP
                     SFGLAGWKAVALLFGLALVARLWALGRADLWCDEILFVGRSHAPKSPLRLLAVCWREF
                     LLVTHLPLPEILQNLFLWLCLPFDPDLIYNNGLQRLPAALWGAGSVALLFRLGAKIVR
                     PATAWVAALSMCFFFYPVYYSREVYYYAPLIFFAAAAFNLYADLLIRRPVTRRAAVAL
                     AVCLSAMALSHLTGILLVFLLFLFHLGWLGCSRIRRANPPPDSPPAPPARSTLWFLPA
                     LLLALPFVLRLALQPVEPLMEGSPLSVPRMLLYVLGKFTLGTARPWGILGVGIMVLGF
                     GELLAKGPQREARRSLGLFTLAGFLILAVWQTRTQFAVRYFTVLLPLFMLIWAAGLET
                     IARSAARLGRVGASGAERMTLGLGAVFILAHLAFFLPPLYQLRAKGRDYGGLARWLTE
                     NLRPGTPYFFEYGGWDSRFVPVYFPTLGLAHAITETGYGPEFHAFLREQQTNLVARFP
                     ESAGIEAAGAEWPPLSRFYRRKHELRNEGIIRLIRKGIWFELPLGDRTPGDLNIWYNT
                     RDDAADLAREAGAPAYFDFEGAECAQMAQDVYVHAAQGNRMTVRVVNLAPAPQRGRFR
                     LEGALNTSQALCPLYVTWQNQLLLQTSCVQRTPWILETPPVELPAGEHELVLRAGSRD
                     EAPVLNVAVLQADFLPVP"
     gene            54481..55188
                     /locus_tag="PK634_02205"
     CDS             54481..55188
                     /locus_tag="PK634_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW96581.1"
                     /translation="MPAGKQTGISVVIPCLDEQAGIGAVVRDALRGIRDSGLPGEVLV
                     VDNGSTDGSADIAQQAGARVVREPVRGYGAALRRGMAEAQQAFLVMGDGDRSYDLADI
                     PRLVEPLWRNEADLVMGDRMRDIRPGAMPWLHRRLGNPLFSLLVRVLFRNNTVHDVHC
                     GLRAIRKESYTALQCATTGMEFASEMVVRAFRAKLRIQEIAVVYHPRVGVSKLRSFRD
                     GCRHLRFVIREAFLAKP"
     gene            55801..55891
                     /locus_tag="PK634_02210"
     tRNA            55801..55891
                     /locus_tag="PK634_02210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55835..55837,aa:Ser,seq:gga)
BASE COUNT         9687 a        19032 c        17316 g         9805 t
ORIGIN      
        1 caggtactct ccgacagcgg ccacgcggtg gaggaactgg acagcgggtc cgcgttcaag
       61 gagcgctggc gcaagaacgg ggcgcggccc gacctgatca tcatggattt cagcatgccg
      121 gagtacaacg gcctgcagct ctgcgagtgg ctcaagggcg agggcggcgc cgtgcccgtg
      181 attctcgtca gcggcttcgc cgccacccag ccggacatcg ccaaggccct gcgcctcaag
      241 cggaccttct tcctccagaa acccttctcg ttccgcgaga tgacggacgt cgtcaccgtg
      301 gcgctgggcg agacgctcat cggggaatga ggagggaggc cccgtcccgc gcacgccgcg
      361 gggcgcggcg gctccaaaga aaacgggcgg cgccggagcg ccgcccgttc ttcacgtcgt
      421 tccgatgcgg atcagaactc gaaccgcacg cagccgccga ggatcgccgt gtcggtgtcc
      481 acgtcgatgt cgccccagtc cgagaactgg tagcggccgt agatgtcgag gtggagggcg
      541 tccggcagga cttccaagtc caggccggcg cgcaggacgt agaacggatc gtccgcccag
      601 tccttgatgg gatcgccgaa gtagtagatc cccgcgccga cgcccgcgta gatccagctg
      661 cccaggatgg cgaacgcctg gggcgcgtag tccgtgtctc cccggccgaa cacgccgtcc
      721 gggaagattt ccaggtccgc ctcgatccgg aacatctcgg aggggcagta ctggtaggac
      781 acaagccagg aaatgccgtc ctcgtcgaag tcctcgtcca cgtcgtcaat ggcccgccag
      841 tagttcacgc cgccgccgag acggctttcc tgggccgccg ccggcagggt gaacacggag
      901 acgaacaaca gcgccagcaa tacaccgatc gctttcttca taggcctctc ctttgttatg
      961 ggcggggctc cctgcgcgga gccggcatgc cgccggttgg ccctttattg tagcacgcgt
     1021 gcggcaggcg caagaaaaaa gtcaacaagc ggcgggttgt ctggtattct tcgaacgttt
     1081 aaccatcgga gcgcatccta tgaaaaagac acccgttctc ctcctggcgg tcctcgcgct
     1141 ggcggttttc acgcttcccg cgccggccga tccgccgaac accgcgatcc tcgacggccg
     1201 gcccacggaa tacgacggcg cggacctcgg cggcacgttc ttcggcgccg cctcctgggg
     1261 cgcgagcgac gtgatcacca acctgtacgt cacctgggac gccgagtacc tctacatcgc
     1321 cctgcagggc tgggaatccg gcaacaagtt cgccgtgctg ctggacgcgg accccggcgc
     1381 gggcaccggc gcgacgacga cgaccaactg ggtgaagaat gccatgccct acatcggcta
     1441 taacgatgtc ggttggcagg cctcggccgc ccccggcgcc gaaaacttcg ggctggactt
     1501 catgatcgcc tcggaaggct actacaacaa catcgtgcgc gtgacgtacg acggcgcggg
     1561 cgcccccgac agcaacaacg tcgaggccgt gttcgaccag ggcaacggcg tcagcccgcg
     1621 cggcacaccg gtcgatatgg tcgtccaggc cgactccacg gcctgcccgc tgaaggggtt
     1681 cgaggcgcgc atcccctgga gcgtcctcta cgccacgaac cggctcggcg aggtccagcc
     1741 gggcgaggtg gtcccgcagg gggcgcaggt ccgcgtcttc gccagcctcc acaacaacga
     1801 cacgaactcg gcctacagtt cgcccgacgc cattcccgcg cagaccaacg cggcggcggg
     1861 tggcggttgg gccgccgggc tcctgaccac cgccaactac gtcacgtacg acgtcgacgc
     1921 ggaccacgac gggttcccgg acctggcccc gggcgacgtc aacgcgccgt tcctgcgctt
     1981 cgtcgccggc gtccagtcca accatgtcgt ctacgcgcgc ttcaacgaga atgtgaccac
     2041 gaacaccgcg accaacacgg cgaactgggc cgtgaacggc gagagcccgg gctccgtgga
     2101 gatgttgagc gacgccacct tcctgctgcg cctgaccaac gccctgccgg cggccggcat
     2161 gctggtgccc gtcgcggccg acttcatcga ggacgcctcg accaattcca agcacacctt
     2221 cctgtgcctg ttcccgacca acgcgggcat atccacctcg gtcaccgtcc gcttcgtgct
     2281 gcagaccgcc tcgggcctcg ggaggacgcc gggcgcctcg aactttttca tcaacggctc
     2341 cacggagccg ctggagtggg gctacccgcc cgcgacctcc atgccgctgg cgttgatcgg
     2401 cggcccgctc acgtcgtact accgcgacgt cacgttcccg cccgggaccg ggtcgcgggt
     2461 ctactacaag tacagcggca tcctgaagaa caccggcacg aacaacttcg aggccatccg
     2521 cctgcagaag tacgccgacg cctcgcgctg gctgaccctg ccgaccgacg gcagcagcct
     2581 cgtggtcacc gactacctcg gcgtggccgc ccacccgtgg cgggatccca acgacactaa
     2641 cttcgacttc tacgccatgg tctacaccaa cgccacgcgc ggcgacgccg gcgtgcgcga
     2701 acgcgtgacc atgaccttcc ggctcgacct ctccggccgc gacctctacg gcgtccagcg
     2761 cgtcctcgtc gtcggcagcg acccgctgcg ggggttcaac cgcaatggca ccggcattgg
     2821 cgactggccg ggtaacagct atgtaggatg ggatgtggga ggaatcgaac tggtggatga
     2881 cggaacgctc ggcgatgcga ccgccgctga cggtgtctac tcgcgcacgt ggagttttac
     2941 aaccaacggg ctggatccgg ccatggtagg ggacacgccc tattcgctga tcggcggaga
     3001 tagttacacc gtgccctact acgggggcgc gtgggccaac cgccggaccc cgcgcagctt
     3061 cgcctacaag tatgcggtgg tcttcggcgc ggaatccgcc gtcctggacc cggcctcgga
     3121 cgtcgagcac tacatcgaaa ccggcagcac gaacatcgtg ctcgacccgt acgtctgggc
     3181 caacagcgaa ctgcccttcg cgccggcggc caacccgccg caactcgtgg ccatgcagcc
     3241 ggtggccggc ggaaccgtcg tcctgttcac gaataatatc ggcgaggcgc agcacggcgt
     3301 ccagttctcc accaacctgg cgcagggctg gacggactac ggacagacgg ccgcgggctc
     3361 cggagggaac tggaccgtga ccgtggagaa cgcgagcaac ccgatgttct tccgcctgta
     3421 cgccggggcg catcccttcc ggggcgtctg gtgggagccc aacccgctgc cggagacggg
     3481 cgggacgatg cggatttact acacgcagta cagccgcggc ctggctggcg accgcaacgt
     3541 gcagatcgcc gggaacttcc cgccctcttc ctggggtccg gtcccgatga ccttcatggg
     3601 cgacggaacc tggtactacg acctggcggt ggacaccaat gtcatcgtca gccccaattt
     3661 cgagttcaag ccgcgcaacg tgtcgggcac cgtctgggag ggaatgggcg gcggcgggga
     3721 caactaccgg gcgtaccgcg gcaccctgcg cgccacctgg acaccgcatt cgccgacgaa
     3781 cggcgggacc attaccatca cctacgacca ggcgggcggc ccgttggcgg cggcgactaa
     3841 catcaatgcc catctcgggt tcgacgagac ctggaacaac ctgaccacca acgtcatggc
     3901 caatatcggc ggcacgacat ggcaggcgac gatccccgtg cccaccggcg cgacgttgag
     3961 cgtgaactgg gtgttcaaca acaagtacag cggcgccgtc accaactggg acagtccggg
     4021 ctacggcggc cgcgactggc gggcgtttat caacaagtag aaggagacat ccatgaccaa
     4081 gcagcaccgc gccttcacgt tgatcgagtt gctcgtcgtc atcgcgatca tttcgatcct
     4141 gatgtccatt ctcattcccg tggccaagct gggtattgag aacggacgcc gggccacctg
     4201 ccgtagcaat ttgcggcaga tcggcgccgc gctgtacctg tacgcgggcg atcccgtcat
     4261 gaacggcgag ttcacgctga tcgcggcgcg cgagggcgcg gagcttagta accagtaccc
     4321 tctgccgcct catgccatgc tgctgggcgg agtgatgcca accaaaggcg tcgacgcagg
     4381 ctttgtgagc aacagggtac gggaaatcct gatcgatccg aaagtatgga tttgtcccag
     4441 cgataaagtg gatggcgaca atctggagcc ggtcactccg gccgtcacaa tcgccgacat
     4501 tggacagcgc aacatcagtt acatgtacat cgcggggcac cggctgatct cgtcaccgga
     4561 aaatgccgcc attgcgccca tgatgtgcga cgaggcgaat gccatggaaa gaggcgacgc
     4621 gacgccagcc aacatgccga aaattacgga cgcggacaac cacggggccg agtaccgcaa
     4681 cgtcctctac ctcgacggcc acgtggcggc gatccagaac gccgacgccg ccaacagcat
     4741 cttcaccaac ctcgtcaaca cggatatcct tcagagcatc gattgacggc tcgaccgcgc
     4801 tcaccggtcc gtgatcgccc ggatggcctc ccgcgcggcg acgaggccga aaacacccgt
     4861 gacgcagcac aggctgccga gcgtgcggcg cggccggccg cgccgcaacg cgccatccgg
     4921 ctccggcggc gccggcgggt tccagggggc ctcatccgag tacacggcaa ggatgcgctg
     4981 ctggagtccc ttcggaacgc ggccgcgaac ctcgcgagcc agcgggcaat cgcgcgtttt
     5041 tgaaagcatc gccactttca ccgccaacgg gtcgccccgg ttggccgcgc ccatgcagga
     5101 aatcacgcgc cgggcgcctg cgttcacggc ggcggtcaac agggcggcct tgggcccgac
     5161 gctgtcgatc gcgtcgatca gcacgtccgg cggcccggcc aggatccggg gatacgatgt
     5221 ctcatccgcg aacaggtcca gcgcctcgac ccggccgtcc gggttgattt cggcgacgcg
     5281 cgccgcggcg gcctcgactt ttttgcggcc cagcgtcgaa tgcgttgccg tcaacaggcg
     5341 attcaaattg cagggccgaa ccttgtcgaa gtccgccagg cggaaccgtc cgacgcccgc
     5401 gcgcgcgagg gcctccagcg cgaacgagcc gaccgcccca aggccgacga tgaccaccag
     5461 ggcgtcggtc agtttctccg cggcctccag gccgaggagt agttcgttgc gcgagaaggc
     5521 gggaggaggt gtcatgggtt caggctttgc agtatccggt ccgcgttttt tcccgtaacc
     5581 cgggccgttt cctccagcgt ccacccccgg agctccgcca gggccgcggc gacatggacc
     5641 aggtgcgccg gctcgttcaa tggcgggacg aaggccgatc cgtcgggcgt ccggataacc
     5701 ggcggcaggt cgggcgcgtc ggtctcgatg agcagccggt ccgccggcgc tcgcgcggcg
     5761 gcctcgcgcc cgcgccggtt gttggagcgg gtgatcgtgc ccgagaagga aaaatacgca
     5821 ttgagcgcgg ccagtttttc catgagttcc gcggggccgg agtaggagtg cagcacgagg
     5881 ccgggctcgt ggcggccgaa ctggcgcagc aactcgggca tccggcccca cgcgcggcgg
     5941 ctgtgaatcc agacgggccg gccaaggcgc cgggcgaggt gcagttgagc gagaaagact
     6001 tcctcctgct cggcgtcgtt ccggtccggc agggcgtggt cgagcccgat ctccccgacg
     6061 ccggcgcggg ggtgttcgat cagcaggcgc tcaagccgcg gaagccattc cgcggagcgc
     6121 tcgcgcaggt accagggatg aaggccgaac gcggggatga tgtccgggtg tcgcccggcc
     6181 aattccacga cgcgattcca gtcggattcc gcggaaccgc aacacaccat tcgttcgacg
     6241 ccggcagcgc gggcgcgcgc caggacgccg tcgaggtccg gcgccaggcg gtcgtcctgc
     6301 aggtgacaat gcgcgtcaat ccaggacaag gcggcgtcgc gggagcttcg ccctccaggg
     6361 accttttcat ggagggtcga gctcccgcga gaccgcaacg attcgagcgc ggcgcgcagg
     6421 tccgcgggca ggggggagtg gaatcggcac tcggttcccg cgcgcgggtg gcggaaggcg
     6481 atctccgccg cgtgcaggaa aagccgcggg atgcccggcg acgcggagcc gtacaaggcg
     6541 tcccccgcga cgggatgccc gagcgaagcc agctgacagc ggatctggtg cgtgaccccg
     6601 gtccggatgc gcacgtcgag gcgcgtgaac ccgggcaggg cttcgcgcgg ctcgtattcc
     6661 gtgatcgccc gcatgggccg gcggccgcgc gctccgtccg gaacgacaac catccggtgc
     6721 gccccgcctc cagcgtgcgc caggagctga tccagccgcc cgccggttcc gatcgcgccg
     6781 ccgacgagcg cgatgtatcg cttgtccaca tccccggcct gaaactggcg gcggaaggag
     6841 cgataggcgg attcatcacg cgccgccagc agcagccccg atgtgtccgt atccagccgg
     6901 tgcgcgatgg ccgggaacag gggatcgggc ccgatggccg acagctccgg atggcgggcg
     6961 atcagggcgc tgacgaccgt gcgcgtgtcg ccgggctcca gcggatggac cggcaggccc
     7021 gcgggcttgt cgagcacgag gaaggctggg tcctcgtgga tcacggcaag gggaagggcg
     7081 gggttcggcg ccgccttcca atcggcttgt tcgagcaact cgacgacccg tacccggtcg
     7141 cccgccgcca ggcggcgccc cttggccgcc ggtcggtcgt tgacgaggat ggcgttctgc
     7201 tcgatggctc gcgccacgag ggaccggctt gtggagggaa aacgatcggc gagaaaccgg
     7261 tccagccgtg ggcccgcgtc cgccggcgat acatcgatcg agaggtcccg gatcatggcg
     7321 gctcttcgcc gagaacccat cttccattga accagtagta ctggtccggg tgcgcccgga
     7381 tggcctggtc gaaaaacccg atgacatatc gcgtcatccg ctcgatgtcg gcgtcgcggt
     7441 cgaacgcggg gtcgggatga atcgggggga agcccaccca gcggtggcgc gtccagcctt
     7501 cgcggacggc gtaggccggg atgaccggga cgccggcctc ccgcgcgaag gcggccatgc
     7561 cggcggggat ctgggccgtg tgcccgagga acggcgccgc cacgaactgg gccttggccc
     7621 gcaggtcggg caggatggcc aggaccttcc catgctggag gcccttgacg atccccgtga
     7681 agctatgggc ctcgcggagg aacgactcga ggcccgcgga ggcgcgcacg cggttgagcc
     7741 attcgtaggt gagcatgttc ctctggcgcc gcacgatgat catcaggcgg gcccccagga
     7801 gttgcatccc cacgccggcc agctcccagt tgcccatgtg cgggaccgcc aggacggcgc
     7861 cgcgcccctc cttcatattt tcgaagagga ggtggacatc ccgctggtcc accacgcggc
     7921 tgatccactc ccgggtgacg aggggggtgc gcatggactc gatgccgttg aacacaaggt
     7981 tgcgccacgc gagccagagg atacgccgcc gctcgcgggg ggatttcgcg tcgccgaaca
     8041 cctgggcgat ccgccgctcg aaacgatgca tccgccggcg ggcgaccagg aagaccagcc
     8101 gggccagcac ccagccgagg cccagcgccg cgcggtgcgg cagccggccg acgatcgccg
     8161 tcaggccgcg caccaggccg tactccacgt agtcgcgaag gcgatgcttc attcggcgcc
     8221 ctccaccagg atccggaatc cgacgtccgc attccgatag gcggccggga agggcacgcg
     8281 gttgtagacc cggaggtagc gggggtcgcg ttcggaccag ctgccgccgc gcgcgaccgg
     8341 cggtccgccg gcattgtccg gcgcgcacca ctcgaacacg ttgccggcca tgtcgcagag
     8401 gccgtagccg ttcggcggga atgcgccgac gcgccggggc gccctggcgt tgaagcatgc
     8461 gcgtccttcc ggcgcgcccc acccccacgg gaaccgggcg ccctccagcc cgccgcgcgc
     8521 ggcgtactcc cattccgcct cggtcggcag gcgcgcgcgg aggccggcgc gctcggacag
     8581 ccagcggcag taggcctgcg cgtcggccag gctgatccag gccgcggggt tccggccctg
     8641 tccgcgcgcc gggcggaagc gcttcccgcg ccgggtcacc tgcggcgagc ccgggtagtc
     8701 cggctgcccg ccgccgttca agcaggccgc ccactcctcg acggtgacct cgtactgccc
     8761 gaggcggaag gcgggcatat cgatccggcg cagttcccgg ggcggctggg cctcgctccc
     8821 gcccatgacg aagacgccgg ccggcaccgc cgcgagcggc ggcgccaccg cggcatcgcc
     8881 ggcctgtgcg ggcgcgccgc cggcggcgcc gagcagcccc agcgcgaatc ccatggttat
     8941 ggcgggtttg cgtattctca tgatctgccc ggctgccgtc gccgcccgat gccgcggggc
     9001 gccgatgtaa aggcttatag ccgcgagact cggctttgac aatctcccgt ggaaccccta
     9061 gagtgaattg ttcattcaaa agtgggggag agcctgcatg aaatgcgatg ccgcgcgtcg
     9121 gaacgggtcc ggaatgcaac tgctgggtct gctgccgctg atagccctcg gggggctgct
     9181 tggctacctg ctggcgggag atgtcttctc cgcttcggtt cgcaagctgg ccgcctcgct
     9241 tagcctcggg ctggtgtggg cctacctctt tcttcagtgg atcgatctcg tggccgagta
     9301 ctgggcgctg aagcgcgacc cggtgccggc ggacctggat gtcgccgacc ggtcggccgt
     9361 ggcgtcgcgg ctggagtcgc tgtgcggcgg ctccgcggtc accacccgcg tgcgccacct
     9421 ggtcaagtcg tggagcctgg gctggcatcc ccggcaggtc atgggcctgg cgggcttcca
     9481 gagcgcccag gcgaagggcc agctctatgg cggcgtcgtg ttcgcgctga tcctgctggg
     9541 ggtggccgtc tggcttcagg ggaactcgct ctggttctgg gccgccatgc tgctgctcgg
     9601 cctgaccgtg ctggcgcgcc agacactgca gctgcgcgtg gatcgctaca tcgagtgccg
     9661 gctcctcgcc tgcctgcccg gcaatattcc gcagaccgcc atgaccgcgg ccgacctggc
     9721 cggcgccctg ggcggcgcga tccagtccgc gttcaagaac catgtgccgc agcccgatgc
     9781 gatggccgcc gcgatgcgga cggccatgga cggcgccatg cagcaggtgg ccggcgcgat
     9841 tgagaagctg cagaagagcc tgggcgaggg gcagggggcc gtggtggaga agtggaccca
     9901 ggcggttcac ggcgcggcca gcgacctcaa gggcctgcag gaagcgctca aggcggccac
     9961 cggcgacctc aagagcggcc tggcgtcggc cagcggcgat ctcaagggca gcctggccac
    10021 gggcgccgag cagtggaaat ccgtcctgca gggccacgcc cagagcctgg ccgccggcac
    10081 ggagcagtgg aagggtgtcc ttcagaacca cgcgcagagc ctggacaagt ccctggccgc
    10141 cgtgccgggc cagttggcca agacctacgg cgagggcgcc ggccaggtcg tcgcggcgtt
    10201 caaggaccac gcggagaaga tggccgcggc cagccagggc atccagaccc agctcgaccg
    10261 gattgcgcag ctcggcaagg agatcgagaa ggtcctgcac gtccagcagg ccgtggatgg
    10321 cacgctcaag gccgtcgccg ccaccgagga attccgcaag acgctcgaca cgctcaacaa
    10381 gcacgtcgag gcgtcggaca agctgctgcg cgaggcggcc cggcccaaga ccatccggct
    10441 tgtcgagagc gacgacgacg ccaagggctg atcgaagaag cggcggcgga gagaagcggg
    10501 cgtccggcgt ttccgggcgc ccgttttatc atccgcccgt caggcttctt ccgccggcgg
    10561 cacgccggcc gggggcggtc ggatcttgtt tacgcgcagg gggaatcgca ccttgagatc
    10621 gccttccagc aggatctcga tccagtgcgt gccctcttcc tggaatacga cgttcatgaa
    10681 ggcctcgatg ttcgtcgccg tggcctccgc gttgggcagg cgcacgggga tgtccttgcc
    10741 ttccgccagc acggacaggc cgtccgggcg caggacgcgc gtgcgctgct tgaatacgcc
    10801 ctcgccgctg caccagcggg tgaccatgaa gagccgcggg tagacgagcg ggaacttggg
    10861 cgcgccgatg gcgtcgaaga gcccgatcag gatgaacttg ccgttccgct cctgccgcac
    10921 gtcatcgcag aggatgctcg actggagatc cggtatcatg ccggcggtct tcttcgttcc
    10981 catggcccga gtatgcgcga gcggcccccg aaagagaagc gggaaagaaa tcggggcgtc
    11041 agccccgcac cttcttttcc ttccgccgct tctcgacgat ggtctccgcg agcgcgtcgg
    11101 ggaccgcctc gtagtgctcg aagtgcatgg agaacgaggc gcggccctgg gtcagggtgc
    11161 gcagcgcgtt ggcgtagccg aacatctcgg cgagcggcac gaggccctcg atgatctgca
    11221 ggtcgccctt ccgggtcatg gactcgatgc ggccgcgccg ctggcagatg gagctggcgc
    11281 acggccccat gaactcctcc ggcgcgacga cctcgacgga catgacgggc tcgagcagtt
    11341 cgggctgtcc cttgaggaac atctgcttga acgcgatgcg cgcggcctcc tggaacgcga
    11401 aatcgttgga gtccacctcg tggtacgatc cgtcgtagag cgagacccgc aggtccacca
    11461 ccggataccc cgcgtacggg ccggagcgca gggccctgac cacgcccttc tcgacggacg
    11521 ggatgtactg cagcgggatg cgccccccga ccacgtcgtt gacgaattcg aagccgtcgc
    11581 cgggaggcag gggctccatt ttcatgcaga cgtggccata ctggccgcgc ccgccggatt
    11641 gcttgatgta tttgccttcg ccttccacgg ggacggtcgc ggtctcgcgg taggccacct
    11701 cggggcgccc caccttcgcc gccacgttga actcgcgttt caggcggtcc acgatgatct
    11761 cgaggtgcag ctcgcccatg cccgagatga tcgtctcgga cgtctcggcg tcgaacgcca
    11821 cggtgaacgt gggatcttcg tccgccagcc ggtgcagcgc ctcgcccagc cgctcggaat
    11881 ccccctgggt ttccggggcg atgctgatgg acacgacggg cgccgggaat tcgatggcct
    11941 cgaggtggat gggctgggcc tcggtgcaga gcgtgtcgcc ggttcgcgtg tcgctgagcc
    12001 ccaccacggc gacgatgtcg cccgtgaagg cctcggtgat cacttcctga cggttggcgt
    12061 gcatgcggaa cagccggctg atgcgctggg tcttgtcgcg gctgctgttc agcacggtgc
    12121 acccggattc caggcggccg gagtagatgc ggatgtaggt cagcttgccc atgtgtttgt
    12181 ccgacaccac cttgaaggcg agcgcggaga aggggtcctc gtccgtcggt tccctcttgt
    12241 tggcctcggg ttcccgggcg cagatgatgg gcgggatctc gttgggggcg gggaggcagc
    12301 ggatgacgcc gtccagaagg cgctggatac ccttgttctt gaacgccgag ccgcagtaga
    12361 ccgggaccac gcggcgggcg atggtcgcct tgcgcaggat gcccaggatc tcgtccttgg
    12421 tcaggtcgcc cttctccacg aatttctcga ggagggcgtc gtcctgttcg gcgcacttct
    12481 ccacgaggtt ggcgcgccat ttccgggcct tttccatctt gtccgccgga atgctctcct
    12541 cccggaatgt gacgccctgg ttgtccccat cgtaatagag ggccttcatg gaaaccaggt
    12601 cgatgatgcc ttggaacgcc tcttccttgc cgatgggcac cacgacgggg acggcattgg
    12661 cgcccaggtc gttctggatt tcctccagga cggcgaagaa atccgcgccc acgcggtcca
    12721 tcttgttgac gaacgcgatc ttggggacgt tgtacttttc gctctggtgc cagacctttt
    12781 ccgcctgggg ctccacgccg ccgacggcgc agaagagggc gatggcgccg tccagcacgc
    12841 gcaggctgcg ctcgacctcg acggtgaaat ccacgtggcc gggggtgtcg atcagcgtga
    12901 tccggtgatc gcgccacagg gtcgtcgtgg cggcggaggt gatggtgatg ccgcgctccc
    12961 gctcctgggg catccagtcg agctgggcgg cgccgtcgtg cacctcgccg agtttatgcg
    13021 tctttcccgt gaaaaagagg atgcgctcgc tgacggtggt cttgccggca tcgatgtgcg
    13081 ccatgatgcc gatgttgcgt atgttcgaaa gctgtacctg tctgggcatg gtgttttcct
    13141 tcccgcgggg gtcaatcgcg gttttcgcga ggtcttcccg gccgtaaaaa ggtcgcgact
    13201 atgccggggg aacccccggc aggcaagaga aaaagcgcta aacccggccg tttcccggcg
    13261 gggaaaaatc tttttttggc atcgggctcc cggatgggct ataaggagtt ccggtaccat
    13321 ggcggaagat ttcggcgatc ccgcggagcg atgaatttga agaccgtgcc gtttgtccat
    13381 ttgcattttc acacgtcgta cagcctgctg gacggggcct gccagccgga ccgcgtgatg
    13441 gaacgggcgc aggaactcgg catgacggcc gtggccatca ccgatcacgg cgtgctctac
    13501 ggcatcgtgg acttctacaa gcaggcgcgc agccgcggga tcaagcccgt gctgggctgc
    13561 gaggtgtacg tggcgtccga cggccggcgg gagcggaaca cgaacgcccc gcggcatcac
    13621 ttggtgctgc tggccgagaa cttgcagggc tggcacaacc tcgtgcgcct cgtctccctc
    13681 gcgcacctcg agggcttcta ctacaagccg cgcatcgaca aggaactgct ggcgcagcac
    13741 cacgaagggc tgatcgggct cgcgagctgc ctgcacggcg aggtggcggc ggcctgcgcg
    13801 cacgaggatc cggccgcagc ggagcgcgcc gcccggtcct acacggagat cctggggccc
    13861 ggcaactttt tcctggagat tcaggaccac ggcctgcccg agcagaagat cgccaaccgg
    13921 ggcatcctgg aggtcgcccg ccgcaccggc ctgccggtcg tcgccaccaa cgacgtgcat
    13981 tacctccgcc cggaacatgc cgaggcccac gaagtgctgc tctgcctcca gacgcagacc
    14041 gtcatgaccg acccgaagcg catgcgttac gcctccgacc agttcttcat gaagagcggc
    14101 gaggaaatgc agcgcctgtt ccccgaccat cccgaggtcc tggcgaacac cgtcgagatc
    14161 gcggagcgct gcaacgtcga gctgcggctg gagagggacc tccatttccc gcgctacgag
    14221 atcccgggcg gcaaggacga gaaatcctac ctcgagcagc tgggccggga ggggctccgg
    14281 cggcggtacg gcatcgagga cctcgaccac ccgaagaacg agcaggagaa aaaaatccgc
    14341 gaccggttcc atttcgagct gggcgtgatc gagaagaccc ggttcctcaa ctatttcctc
    14401 gtggtctggg acttcgtccg gttcgcccac gagcagcata tccctgtcgg gcccggccgc
    14461 gggtccggcg cgggcagcat cgtggcgtac gcgctgggca tcacgggcat cgacccgctg
    14521 cggtacaacc tgatcttcga gcggttcctg aaccccgagc gcatctcgcc gccggatttc
    14581 gacatcgact tttgccaggc ccggcgcggc gaggtgatcg actacgtccg cgagaagtac
    14641 gggcgcgaca acgtcgcgca gatcatcacc ttcggcagcc tcggcgccaa aacggtcatc
    14701 cgggacatcg ggcgcgtgct ggagatcccg cttggcgagt gcgaccgact ggccaagatg
    14761 gttccggacg atcccaagat gacgctggaa atcgcgctca agcagaatcc cgagttccgc
    14821 aaggcccgcg acaccgagga ccacgcgcgg cacatcctca agtacgccga ggtgctcgaa
    14881 gggctgcccc ggaatcccgg cacccatgcc gccggcgtgg tgatcggcga aaagcccctg
    14941 atcgagctgg tgccgctcca gcgggacaag gatggcgagg tcatcacgca gttcgagatg
    15001 gagcccctcg gcgagatcgg cctgttgaag atggacttcc tcgggctgaa gaccctcacc
    15061 gtcatccagg aggccgtcga cctcgtccgg gccgggcgcg gcgtcgagct ggacatggcg
    15121 aacctgccca tggacgacgc ggcgacgttc gacctgctca accgcggcga caccgtcggc
    15181 gtgttccagg tggaaagcaa ggggatgcgc gacctgctgc ggcgcatcgg gctgacgcgg
    15241 tttgaggatc tcatcgccat gatcgccctc ttccgccccg gccccatgaa catgctggac
    15301 gactacgtca accgcaagca cggcaaggtg aagctcacgt acgaccaccc cctgatggag
    15361 ccgatcctgc gggagaccta tggcgtcatg ctctatcagg agcaggtcca gcaggtggcc
    15421 aacgtgctgg ccggcttcag cctgggccag ggcgacatcc tccgccgcgc gatgggcaag
    15481 aagaaaaagg aagtcatgga cgagatggag gagaaattcg tccagggctg ccagaagacc
    15541 aaccacgttc cgccccgcgt cgccaagaag gtgttcgagg acatctcccg gttcgccggc
    15601 tacggcttca acaagtcgca cagcgcggca tacgccgtcc tgtcctacca gaccgcgtgg
    15661 ctcaaggtgc actaccccgt ggagttcatg gccgcgctgc tctcgagcga aatagggaac
    15721 acggacaagc tgcccttgct ggtggccgag ggcaaggaga tgggcatcga gatcctgccg
    15781 ccgaacgtca acgacagcga cgtgcggttc aagcccgtcg gcgacgccat ccgattcggc
    15841 atggcggggg tgaagaacgt cggtaccggc gccgtcgcgg cgatcgttgc cgagcgcacg
    15901 gcgcgcggcc cgttcaaggg cctggtggat ttctgcgggc gcgtggacgc ccagctcgtc
    15961 aacaagaaga cgctggagag cctggtcaag tgcggggcct ttgacttcag cgggatgtcg
    16021 cgcgggcggc tcttcgccgg cctggacttt gccgtgagcc acgccgcggc catgcagcgg
    16081 gacaagcgaa gcgggcagac ctcgctcttc gacctcctcg ccccgacgga agggacgccc
    16141 gtccaggacg agttgccccc gggcgagccg tggccggaaa gccagatgct ggccgcggag
    16201 cgggagctgc tgggtttcta catttccggc cacccgctga ccgcctacga atgggcgctg
    16261 cggaccttcg gggtggcgaa catggcgggg ctggaagccg tgccatccgg cggcgccgcg
    16321 cgggtcggcg gcctcgtcgc gcagttccag aagaaattca cgaagaaaga agagaagccg
    16381 atgggcgtgt tccggctgga gctgctcgac ggcgcggtgg aggcggtcgc cttcccggag
    16441 gccttccgcg agtacggggt gcacctccag gacgccgcgc ccgtgatgct ctgcggcgtt
    16501 gtcaagaaag gcgaccagac caagatcgag gtccaggaaa tctacccgct aaaggacgtg
    16561 caccggatgt tcgccgagcg tttgagcgta cacgtctccg ccgccgggct cgacgacgcc
    16621 aagctctgga aaataaagga attgctggcc cgtcatcccg ggccgacgcc ggtcacgctc
    16681 tgcctgcagt tcccgacggg cgaaaaggta ttcgtcaagg cggatcgcac ctttcacgtc
    16741 gaggcggcgg aggctctggt ccgcgagctg gagcacgtgg tgggcgagga aggcgtctac
    16801 gtcgaggtcg tcaagcgcgc gtgtcgcaag aaaaacggcg cgcgcaacgg gcggggccgg
    16861 tggggcgggc gcccggagtg aaggcggcgc ggcggcgtca gtggatactc agccagtcgc
    16921 gcacggcccc ggcggccggg ccttccggtt ccagtctcag gaaggcctcg tagtgccgtc
    16981 gggcgagtcc gtaggtgccg gcctttcccg cgtagagaag gccgagcgca tagtgtgacg
    17041 ccgcgtggtc cggatgggcc ttgaggagca gggtgtactg ctccgcggcg gcttccggtt
    17101 cgccctgctc ggtgagcagc acggccaggt tgtagcgggc ggccacgagg tcgggctgcc
    17161 gctccagggc cagccggtaa gcgtccttcg cgagatcgaa atcccggcgg gaggtgtaga
    17221 cgatgcccag gttgaaaaac gccgtggcga aggagtcgtc gttctcgacg gcgcgcaggt
    17281 agtagaagat ggcgcgatcc aggtcgccct gctgctggta ggtggtgccg cggttatatg
    17341 cctgcacggc cgcctgcagg ttcggggtgg ccgggggctt gaacggcacg tgtcgcgcgc
    17401 cgtcggcgtc tgcggccgtc ggcgccgggg cctcgggccc gggggccggc gcgggttccg
    17461 ccgggggccg gcgcggcggc tccgtgcggg gcggctgcag gatcttgagc cgctcgcggg
    17521 cgcgcacgac gcgagggtcg ccggggaatc gacgctcgaa ctcgcggtag gtccgcgccg
    17581 cgggctcggg catgtccagc ggatcgtcgt acaacgtggc ctggagccac agcgcttccg
    17641 ggttgtccgg ctcctgctgc acgaccgcgc ggcaggccac gagggccgca tcgaattcgc
    17701 cggccttgac cgccgcctcg gccagcccga gttgcgccag ggagaaccgc gggtccagtt
    17761 cgaccgccgt cttgaaacag tccagcgccg cgtccgcctt gtcctgcccg gccaggaatc
    17821 gtcccagcgc ataatgggcc ctcgcctgct gcggggccag ccggaccgcc tcccgcaggg
    17881 ctttttcctg cttacccgta tcgcgttgcc gctctgcccg gcccgaggcc tccaggcaga
    17941 gatgaagcgc ggaggacaca tgcccccgcg aggcctcgtc ggcggcctcc ttgagcaagg
    18001 cctcgaagct cttcgcggcc ggggcgggcg gcggggcggg ggcgggcgtc ggcttttccg
    18061 ccgcgagatc cccgagggcg tgttccgcat attccgcctg ggggcccgtg cgcgcgaccg
    18121 gatcgttgcg caccgactcg atgtacttct cgaaatagct ttccgcctgt ttcgcgtcct
    18181 gtccttcatg cagccgggcg aggttgaaga gagctggcgc gtagccggca tccttttcca
    18241 gggactgcat caggtagaac gcggcctggt ccggtttccc cgcgcgggcc tcgacgcagg
    18301 cccgcgcgtt gaggatgcgg gcggactgcg gggcgcgcgc ctgcgccacc gacaatacgc
    18361 ggcgcgcttc cgcccactgt ccggcctgcg cgtagatggc gccgatgaat tccagcgggc
    18421 gcgcatcgcc aggccgggcg agcgcggctt cctggaagag cggctcggcg cgggccgggt
    18481 cgcccccctc gaagaagatg acccctgtgt tgtagagcga ttccgccatc ccgggcttca
    18541 gccgctggca ggcctcgaag gcttccaggg ccggctgcac ctggcccatg cgccagcagg
    18601 cgacgccgag gtaatggtag gcgacggcgt tatcggcgtg gcccggccgg cgggcgatcg
    18661 atttttccag cagggcccgc gcccggaccc agcgctcgcg ctggaactcg gccatgcccc
    18721 gatcgaactc gcgctccccg ggcgcgcctc cgcacccccc gcagaggccg gcgcacaagg
    18781 cgatcaggcc gaacgctccc gccagcgatg ggaaaagggg ccgcatgatt attacgtctg
    18841 tacctgaagg ataacgcagc ggtgaggtgc ggacaagaaa aaggcaggct tcttcacccc
    18901 gctggcccgg actgggctga ccatcgtccc gttccgtgcc gccggcgcca tagcaaggcg
    18961 gcgccccaca gcagcaggcc gatccccagg atgcccgtca acagaaagcg gctctcccgg
    19021 ggcgcgtggt gagttccatc gtacaggccg ccccagagat cccggtacgg gatatccgac
    19081 gacgccccct tgccatagat gaagaacgac agcccggaca tcatcaagcc ggcgagcagg
    19141 cagcaaaagg cccacgccct gcgcgatggg ccgtccgtgc gatctcgcgg ccccgacgcc
    19201 gcgcccctcg atggccctcg cggttctggc agggggcgat gtcccggccg gttaacgttg
    19261 cgtgcgtcgt tcatatggcg gccaagcgac ggaatgaaca cacccgagcc tgtttattgt
    19321 atgagcgtta cgacgttcac gcaacaagcg cttctgaaca cacgagtcgc actggctact
    19381 cgcgtgtcag aagacgtgac gttttgccga atcaggcatt cgacaaaacg aacgtggcga
    19441 gccccgtttg cgcccgcaac gcgggcacat caccgcgtac ggccacgttg ccgcggccta
    19501 cgcggctcac gtggcggccc ttcgggcgca ttttgcacga cagctcgcgc aaaatgcggg
    19561 ttaacgcgca acccatacgg ttcgcgtccg ggggccgtcg aactcgcaca ggaagagccc
    19621 ctgccaggtt ccgagccgaa gccgcccctc ggcaatgatg acctgcgccg aggaccccat
    19681 gaggctggcc ttcacgtgcg cggccgcatt gccttccgcg tgggcatagc cggcccgcca
    19741 tggcaccacg cgctccaggg cggcgtccat gtccgccatg acgtcgggat ccgcgtgctc
    19801 gttgaccgta acccccgccg tggtgtgcgg cacgaagacc gttacaacgc catcccgggc
    19861 ccccatcgct tcaacggcgg actcgacctt gcgggttatg tccacgaatt ccgtgcgctg
    19921 ccgcgtggaa acgctgaacg attgcatgtc cccgacgatg cgccgccggg cgctggaaat
    19981 caagccatag ccggttgctc ctttctccca tgaacgcagc gctacccgag ccgtgtctgg
    20041 gcaggcgacg gcctatcaat agaaagaaga caggcgggcc ccgttccatg aaaaagggaa
    20101 agaaacgagg gggcggggga ttagcaggat tcgacggggc ccgggcagga ccgggcgcgt
    20161 gccgggtgtg ggcgcgaggg ttttccggcc ggccatgttg tcccgcgatc ctggtcatat
    20221 tcggcctcga cgggtgaaca cgactacgct agcgtgttgc cgacatcgcc ttgaaaggag
    20281 ggcttcgtga aactcattgc ttgtgcggga atcgtggtta tcacgctggg gctcgtgtcg
    20341 tgcgggggcg gcaagtacgc ggacgccaag gcggcgatga atgaccaaat cgccttcatg
    20401 gacgaattcg tgtcccaggt ggacaaagcg agcagcgcga aagatgtcgc cgccgccgtc
    20461 aaccgctatt ccgagcagat gaccaggatg ctggaacaga gcacgaagct caaagaaaag
    20521 tacaaaggcg ttgacctgga aaacgcgccc gagctgaaag aggagatgga aaaggtcaaa
    20581 gcctccgcga tgaaatttgc ccaagccttc atgaagatcg gcacgcagta cggcaacgac
    20641 cccgaggtcc agaccgccac ggccaaatgg cgtgagacca tgaccaaggc tgcgcagccc
    20701 tgacattcat attgcaataa aaatcgagca cctgtggtcg catgcgtgtt ctattataga
    20761 acatacatgg gccatgacca tgtcctcatc agcgggcctg ccgatccttc gggtgccgcc
    20821 ctgtcgatca ggattgtgcc ttcccggata ccgaggccgc ggctggcatc ccgaatgcgc
    20881 ggcccgtttc gggcccgccg cgcgaggcgc tccgcgcgaa gcctcgggcg gtgttgcagc
    20941 gactgattcc agcccgccgg gctaatatca gcattgcttt ttcccgcccc acgccgttct
    21001 ttcgtccgac cttctcctgg atgtgacgta atcctgcctc gccaaccagg gcagcggccg
    21061 cgttttccgg agccaatcgg ctgggcaccc cgggctgaac tcggccactt tttgtaggcg
    21121 gccggaaagg gtggccacgc cggaatccac gcgtcgcccc gcccagcgca ttctacaggt
    21181 ctttgtgcat cgccttgcca acgcggcttc cgagggcctc aacaacaaga ttcagaccct
    21241 gatcaagaaa gcctgcggct accgaaaccg atcacgtttc aagaccgaca tcttcttcga
    21301 ctgcggaggg ctcgacctct accccccttt tcagccagtg aaatcccccg cgaacatgtc
    21361 cgaagaactt ctactgtgag gacattcgct acacgctgcc gcggcatttc cgcgaagacg
    21421 cgtggtaata agcgacatcc cccgccaatc gtggaaacac gttgcacgct tgcatccatg
    21481 tgcgggcgga tgaagctgaa ggggttgtca ggcgaggcca tgtcgcatat aaatctggca
    21541 acgcaaacag gagctccaaa tgagcagatt cagccgccgc atctcgtttt ccaatcattg
    21601 gaaaaaatcc gccgggattt tccaatcatt ggaaaatacc cgcgttcttt ttcccatcat
    21661 tggaataggg ctgctgggtc tggcggcgac ggcgagcgcg cagacggatt ttgaaaaggt
    21721 gacgaacgcg gttcatacgg tacggctcga actggaggcc gcgctgggca agccggtgcc
    21781 ctcgttgagc ctgttggttc agacgccggc ggacacgttt ttcacctgct ccgccgccac
    21841 gccggagcag gcggtcgcca ccaacacgac cttccgcttc gccagcaaca ccaagaattt
    21901 cactgccacc gccgtcctgc atatgcagca acagggctgg cttgatatct ccgatccgat
    21961 cacgaacacg atccccggtt tttcagaacc ctacattccg accggcacca actgggccat
    22021 cccgaacaag ccgatcatta ccattgagca actgcttcgt cacagcgcgg gcgtgtacga
    22081 tgtggataac gatgacgtgc cgggctgcgg cggcacgagt tacacggact acatgatcac
    22141 cctcgacccc gcgcatcaat tcaccgtcga ggaaatggcg gggcaggccg cccttcatca
    22201 gctttcctat tctgcgccgg atgagggcta tcactacagc aacacgggat atgccatgct
    22261 cgcggaaatc gtcgggcgca tttattcgct gcacagcgga agcacgaaaa aactgacgga
    22321 ctatctgtat gatcacatca ttggcggcgc ttcgcccgtg ccgattccgg aaatccattt
    22381 tccgaatctc gccaccgaca ccgcgctgcc ggagccgttc atgtggggga ccgagcggcg
    22441 tccgacgaat atcacgatca ttgtttccaa ctgcaacatg agcgcgcagg tgggcgaggg
    22501 gaacggctac ggcaccctgc ccgcgctgaa tcgcttcatt cgcgccacca tgaagggcga
    22561 aggcgtactg agccatgagt cggtgcggtt gatgcagacc gacacctcgc cgctcaatgc
    22621 gggctatgcg ctcggctgca cgtttgtgac ggatctcggc ttcggccaca acggctgccg
    22681 cgtcggcaac ttgtcgcaca tgaactacaa tccggagacg gatgtttcgg tggccgttta
    22741 tttaccgctg gtggattaca gcgacaacat ggattcgttc acgctctgct ttcaggcaat
    22801 ctacaatgcg gcttaccgcg ccctcgaagc cctcggctac ccgcgcacgc cgaccctcgc
    22861 gcacggctcg cagaccaacc tgaccctgtc cgcaggcgcc acgaacacgt tttatttcac
    22921 cgcggcgcag ggcgcgttct acggcgtgaa aatcagcaac accgtcgcgg atatattctg
    22981 ccgcgtggcg ccgggggtgg atccggacga tgccgtgacc gtcaccaaca acttcacctg
    23041 gcagggccat gcgccgggga cgtatcgtct ggacgtttct tcgaccgcgg ccacggaatg
    23101 cgcgctgtcg ttgattgcac tcaaaatgcc cgatccgtcc gacttcagcg cgctcaactg
    23161 gacgaacgcc ttcatcgccg cgcacgatgt cttttcgcgt cagtatgcct tcggagactg
    23221 gaaatccgtg gactggggcg cgctctacag caacaccttg ccgcgcatcg ccgcggccca
    23281 ggcgctcacc aacgaggtgg cgtactacgc ggcgctgcat gaatatgtgg gctccgtccc
    23341 ggacggacat gtcgcattga agggaaccaa tgccgcggtc cccgccgcct ggggcgagga
    23401 ggtcgcaggg ggagggtatg ggctggccgt ggcggagctg gatgatgggc gcgtgatcgc
    23461 ggcggctttg ctgacccatg gtccggcgca gctcgcgggc atggaggccg gcgcggaaat
    23521 cgtgagttgg gacggactgg cccctgcgtc ggccatggcc caaatcaatg cggcggcgta
    23581 tccgctgcgg gcgctggccg ggcccatgac gagctgtccg caggccaccc tggcgcaaca
    23641 acgcctcgaa caggcgcggc tgcttgtgcg cgggccggtg ggcaccaccg tggatgtgca
    23701 gttcatcaat cccggctcgc tcgccaccca agccgtcgcg cttaccgcca ccgccgacac
    23761 caatgccacc ttcgcgctcc tggactttgc catgaggcaa aatccctcaa gtgatcccgt
    23821 agaatatcgg ctcatgacca acggactggg ctatctgctc atccgcttcg aagccaactt
    23881 tgcggaacct gaaaatctgt acgtcatcaa cagcaatgtg gccgaggccg tgaaattctt
    23941 tgtcgcttcc aatgcgcccg gggtcattgt ggatgtgcgc ggaaatctgg gcggcgacga
    24001 tcaactggcc gcgacgctgt gcggctactt ccaccggaca aacatgtttt acgaaaggca
    24061 gacctggtac aacaccctca acggcacgtt cacggaactc acgtttgatt cgatgaacgg
    24121 atgggcgccc ctggattaca tggccatccc aacgcaatcc gtgttttacg gcgggccggt
    24181 cgccgtgctg gtcaatctgg gcacggtcag ttcgggcgaa ggggtggcca tgggcattgg
    24241 agcgctgccg aatgcccgcg tgatcggctt tcacggcacc cacggctcat tcggtatggc
    24301 cggggccacc attgcgctgc ccggcggcta ctccatccac tacccgttcg gtcaatcgct
    24361 ggacgaaaac ggggtcatcc agctcgacag ccgaaacggc gtcggcggcg tgcctccgga
    24421 aatccgcatc ccgaagtcgg cggaaaatgt gctggccttc gccgcgggaa ccgacgtgga
    24481 attgaactac gcctccgact atttgatcgg gttgcgaaat tacaccaaca ccatcgcgca
    24541 aaccacggcg ctcatcacta atttaatggc ccaacatgat attccggcat tgagcattgc
    24601 gctggtggac ggcgccaaca ttgtctgggc gcagggcttc ggaacggcca accgggaggc
    24661 cggcacgccc gcctcggcgg acaccattta ccgtattgcc tcatgctcca aaacgtttgc
    24721 cgccgcatcc atcatgcgct tgcaggacga tgggctgctg acccttcagg acgatgtcac
    24781 gaatactgtt cccgcttttt ccctgctccc gcgctatccc gaccaggcgc tcaccatccg
    24841 cgaactgctc gcgcaccagg cgcagcttcc gggttgctac ttccgccatg ccgattccac
    24901 cattccctac gatggctatt acgacatgtc gctcgcgctg atggtcgagg actacctggt
    24961 cgagccgcgg cactttctgc aaaaatacaa caacaatggg ttcacgctga tcgaaggcgt
    25021 ggtggccggc gccaacacca acggcctcga ctacgccgcc tttgcgatca cgaacttgtt
    25081 ccagccgatg ggcatgagca acaccacctt ccgtcttgcg gaccaccaga ccaacctgct
    25141 ggccaaggtg taccggagcg gcgtcctcgc gccggatgaa tttgtcaaca cgcccggctc
    25201 cggcggcgcc tattccaccg ccgccgacat ggcccgctat gcccggttcc tgctgggcta
    25261 cggaacattc aacggaacgc gcatcctctc gtccaatgcc gtcgcggcct tgtgggcgga
    25321 ccaaagcgca caggtggccc tggtgaacaa ggaggattat gcgaagagcg gactgggctg
    25381 ggaccagatt gccgatccgt cgtttgccta tgccgggagg atggcctgga aggatggcga
    25441 cagcctgaac tacagcgcca ttatttgcgt catgcccgag catggcctgg ccgccgtggc
    25501 gctcaacagc attcccggcg ccgacaccgc cgccgcgccc gccgccaagc tggccttgaa
    25561 actggcgctg caggagcgcg acggcattcc gctgccctcc acgaatccgc ccgcgttccc
    25621 cgtctcgccc gtcacggtgc tgaccgagca ggagctggcg ggcatcatcg gcacctactc
    25681 cgccgcgggc ggctatgacc tcgtgaccgg cacggtgtcg gccatcacct ggatcaccgg
    25741 cgccgacggc tcctcgcccg tccgctacga ggggcttgtt ccccatcagg atggatggtt
    25801 cagcgcaacg aatgcgcccg aattccgcct gtccttcacc aacatcggcg gacgcgtcat
    25861 cctgcgcagc cacgtgcggt atgaagaata tttgcggatc atcgcacagg ccgagcggtt
    25921 cacgcccgcg ccgttatccg ccgcctggag caaccgcgtc ggcaaaacat ggattaccgc
    25981 cgatctcgag cctgaaagct atctctggct cctgggcttt tacccaacgc tcgaactcgg
    26041 catggccgat ggcgttctct ccgtttactc gtcgccgtgc caaaccctgc aaccccaggg
    26101 cgacgacctg gctttcccct tctccatgaa tgccgctcaa ccctcggcct tgcaggtgaa
    26161 acacaccaac ggccatgaag tgatccgcta cagcggctac gatttccgcc gcgtggacga
    26221 cctgccggtg cttcagcccg gacaatccgc caccggcctc ctggccgccg gcgcttccgc
    26281 gcctttccaa ttcagcattg ccgatccggt ttggcatggg atccatatct ccccctccaa
    26341 cctgattctg cgacaggcaa cggcggacct ttccgcgccc ggaacctatt cgctcatcct
    26401 gcagaatcct ggaacctccg ccgcgcctta ttcgctgcgc ctctacaatc tcaccagcac
    26461 catcgcccgc gtttccacat tgatcaccaa cctcatggcc acccaccaat tggttggctg
    26521 cggcatctcc ttggtggatg gggatcagac ggtctggcaa accggcttcg ggtacgccga
    26581 ccgcgagcgc gggcgggccg cggacgcccg gacccccttc atgatcggtt cgatctcgaa
    26641 gactttcgga gcgctggccg ccatgcaact ggccgaggaa ggcttgctcg atcttcacgc
    26701 ttccatcacg aacgcgctgc cctcctttgc gattcatcaa cggtttgcgg acaacaccat
    26761 caccccgcac acgatcctga cgcatcactc cggcctgcct ggcgacgtgt tcaatcaggg
    26821 attcaccgtg cgcccgctgc cgggcgttcc ggacgccgtc caggccatgc tcgccaacga
    26881 atacgcgctc atgcccacca acaccatgtg ggcctataac aacagcggct tcgtcatgct
    26941 cggccagatg ttccgccacc tcaccgggca ggaactcgat gtcttcgccc gcgcgcgcct
    27001 cttcgaccgc atgggcatga ccaactcctc catcgcgttc gatctgcccg ccatcaggca
    27061 aacccgcgcg cgcccgtatg tcggcggcca gatcggcccg gatgaatatg tgaatctcta
    27121 tttcgcgggc gccatttatt ccaccgccgc cgacatggcc cgctacatgc gcatgctgct
    27181 ggccggcggc atgggcgaaa acgcccgcgt ggtttccaac cagaccctgc aggccatggc
    27241 cacccggcaa aatgccggca tcgcgctgga tcaatacgcc agcatgctga acatgggcat
    27301 cggcttcgta ctcgatccgc ccaacctgcg ctatatgggc aaggtccttt ggcatgatgg
    27361 cgggaccgtc tattttcgca gcctgctgcg ggtcgccaca gaggcgcagt tgggttgctt
    27421 catttcctgc aactcgtatg aaggcggcgc catgacctat acggttgtgg atagcgccct
    27481 ccaatgggcc tacgaggaaa aaaccggcct cgctccgccc gcgcccctcg atcccggcgc
    27541 gcctggcggg gcggtggcgc cgcccgaagt gatcacgctg gccactggcg gtgtcttcgt
    27601 gaccagcggc ggatacgata ccttccgcta cgatggaacc aacctgtttg cgcggttcaa
    27661 tgcgcagttg gacgaatccc cagaaagtgc ctggacctat cgcgaaaacg gctggttcac
    27721 gcccaccaac gcctacgcgc cgcaactgga attcgtccag gtggagggcc gcatcctcag
    27781 cgtgatacaa acgtttgatt atgcggtaac caacctgatc ctgctcggcg agcgcgccgc
    27841 cggactcgcc ccgttccatc ccgcctggag caaccgcctt ggccgctggt gggccacgga
    27901 cctgcatccc gatgatatcg cctggcatgg cgatgacctc cgcctcgcct ggcccgcgct
    27961 gcaactcacc gcgcgcgaca acatgctgct cgtgcaagtg gaggacctct acgtcatgag
    28021 cgccaccaat gacgcccttg cctttgccgc cgggcttggc cgcaacaagg gctccagcct
    28081 gcgcgccgcc ggccctgatg cgatcgctta catgggggtc cactaccgcg ccgaggctgg
    28141 aataccccaa ctggcgcccg gcgcctcgac caatggcagc acccttgggg acgaaatcca
    28201 atggttccag atccccgccg ccgccgcttc gttgacgttt gacctcgcca ccgagcatga
    28261 cctggccgcc tacctctaca cagaggatga cgaatacctc ggccaggcca accgttccca
    28321 tgccctccat ctcgacgcca ccaacgccca gcccgtcatg gccgcgatcc tccgcaacgg
    28381 caccaatacc ggcccatgga ccctcgccac gtacaccaac gccattccct tctactcgca
    28441 agtccccgcc gatgagtggc ccgccgcgct cgccgcgcac tcgaacctct tccccaacac
    28501 cgatttcggc catgtcttcg cgcgcgaaaa tcgcgaaagg ggccccggcg tctcgcctgt
    28561 gctcaaaatc gccctcgccc gcatgaagtc gcccaatccg tccccgcgtc cgctcttttt
    28621 cctcaacggc ggccccggcg acagcggcat ccgttgcgcg tatcagtatt ttctcaaagc
    28681 cttcaccggc acccacgatg tttacctgat tgacccgcgc ggcgtcagct actcgcagcc
    28741 ctccctcgac ttctttgccg acgaagacct tcccgaatgg caataccgcc tcaccatgct
    28801 gcaacaggcc gacctttccg cgctcaacac gctcgaatcc agtcacgacg tggacgacat
    28861 cgccgccgcg ttcggcgtgg cggaagccga cctcatcggc caatcctacg gaaccctgct
    28921 cgcgcaaacc gtcatgcgcc gcgaacccgc ctggctgcgc tccgtcattc tcgatggcgt
    28981 cgtcgccccc aacatccccg gcctaagtca ggccggcccc gtccgcaacg aagcgctgga
    29041 aaacctcttt gccgacgtcg ccgcccatcc ccaggcctcc gtctggtatc ccgccttcgg
    29101 acttgccctc cacaccctct ccaccaacct ccaaaacaac ccggtggatc tggtcatctc
    29161 cggtgaaacc aaccagatga acggattgag cttcctggac gccgtcttca accagctcac
    29221 ctccaccgac ctggccgcgc gcgaacgcat cccgaacatc gtctggcgcg ccacgcgcgg
    29281 cgaaaccgcc gccctcgccg agctcctcac cgagttccgc aaggacacca acctcttcat
    29341 gacaacctgc aaaaacatgg agcagcaaat cctggtcatc aagcacgact tccttccctt
    29401 cgactccctg gaagccgcct ccaacgcctg cgccaatctt ccaccgcttg tcgcccgaac
    29461 cgccctcgac ttcatgaccg acgccatcga aggcgcggcc ctgttcgacg actacggcca
    29521 ggccgacaac gccttcacca atcccgtcac cgtctcgata cccacccttg tcatcaacgg
    29581 gacctacgac acccagaccg gcaccaactg ggccgccgaa gtcgcccgcc acctccccaa
    29641 cgcccattat gtcgcccccc ccgctgtcgg acacggcgtc ctctttgcca ccaacggctg
    29701 catgacccag ctcttccgcg acttcctcgc cgaccccacc aatccccccg acacctcctg
    29761 tcttgccgga ctcgtccccg acttcccccc gccctggccg gcaaacacgc gggacgtctc
    29821 cgtcggccaa accttcacca acgcttttga ggcgcccgac agcgcgcaat ggaaccgctt
    29881 taccgccgtt agcggcgtgg tttataccgt gacgcaaacg ggcggcgacg cgctgcgcgt
    29941 ggtggatgaa aacgccaaca cgttggcctc cgtccaaaat gaggccgtga gttgggaaag
    30001 cagcggcgat gggcggatat acctgatggc gatcgcatcg cgggccggaa caaactcgat
    30061 caccttggac ctgccgctgt tgttccgcca tttggctttc aacggacagc gcgccacctt
    30121 gaactggcaa tcgctgacca acctgtccgt gaacctgtgg atgatcacga atcttggcgg
    30181 taacgcggaa cccatatgcg tccattccaa cattgccgca cagggatggt tcaccacgct
    30241 ggaccatgaa gacgaaaccg cgcatccatc cgcattctac ttcctcaaag acacggaatg
    30301 agctccaagg cctccgctga aaccagcgga cgcgggaact caccgacgcc gggaaatcct
    30361 cttgccggcc tatgttgcgg ccatacccac catggcccgc gactggcgca tccccccgtt
    30421 tccgcccgct ccattcctct gcatgatctt ttcaatatga tttaattttt cccgcagatt
    30481 aacgtataat attttcttaa tatcgaaagg agcggagttc catgcaacaa gtgccggcgt
    30541 caaggatcat cggatggatg gctgtcatgg gcatggcggc cccattggtt tggacttcaa
    30601 cggccttttc cgaaacccaa gcggaagcgg atgtccgggg ggccatgatc gaagtggtcg
    30661 ccgccgcgag ccgcctggat gccgacggca tcatggcgcg gctcgatccg gatccggccc
    30721 tgcgctacta tgtgcagggg caatccttca attatcccga cctcgccgtg ttcctgcggc
    30781 aatcctttgc cgacatgacc acccaacgag tcgtctggct ggaaagctcc gcccgcgagc
    30841 tggccccgga caccgttctc tggacctcct acggacgcaa tcccgtcgtt gaagcaagcg
    30901 gaaaagagat cgaatacatc ctgggggaaa cctgggtttg gaagaaaacg gacgggaact
    30961 ggcgagccat tcactatcac gagtcctttc tggaagcgcc cagccgggaa aaacgcgaac
    31021 gcgtcgaatc ggccctgtcc gaattcgccg cctcgctcaa gattgaatcc cggaatccgg
    31081 aacccgtcat ggccgtcctg aacgaattcg tccggtcgca tccggaggtg gtgggctccg
    31141 ccttcgcctt tgcccctacc gaggaaggct cgtggcccgc cccttacgtt catcgcaagg
    31201 gcagcgaaat catccgcaaa acgttgccgt ccgattcggg gtacgagcga agcgactggt
    31261 acgccgttcc cgccgcaagc ggccaacccg cctggtccga gccctatttc gacactcacg
    31321 gcgcccaaac gatgatgatc acctactcca tccccataac cgacacgacg ggcgacctgc
    31381 tcggcgtcct gacggccgac gtggcctttt actaaagcgt gagctcatga ccacctccaa
    31441 tccagatgac cgaacaagga tactgcgcac ggtgcgcggg caggaagtga cgcccgatcg
    31501 cgatgtgacc gccgcctgtg gcgtagaggc caaaaacctc aagagagccg ttttccccgg
    31561 aaagtacatc caaggccgcg gcgcgttggg cgaactgccg ttgcttatgg attcgctggg
    31621 cgggaaaggc ttggtcctgg cctcgccctc tgcccacaag aacatccttc cggcatgcgg
    31681 aatcgactgg ggctcccgga acacgatcct cgattccttt cagggcgaat gctgcgaaag
    31741 ggaattgtcg cgggtggccg aactgatcgg gcgacaccag gtggatatcc tcgtcggcat
    31801 gggcggcggc aaggccatcg acaccgccaa gatcgccgcc gaccgtgcgg gaatcccggt
    31861 catcatcgtg ccgaccatcg cctcgaccga cgcgccatgc agcggttgcg cggtccttta
    31921 ctcggacgat catgtcttcg agtccgttta ttatcagaag atgaatcccg cggcggtgct
    31981 ggtggacgag cggatcatcg tgcaggcgcc cgtgcgcttt ttcatcgccg ggatgggcga
    32041 tgcgctcgcc acatggtttg aggctcgctc gtgctcgacc acccaatcgc ccaacgcctg
    32101 cggcggactc agccccatgg cgggacttca catcgcccgg ctctgctacg agaccctgat
    32161 cggctacggc gtggccgcca agcttgcctg cgagcaaaag gccatcacgc ccgccttctc
    32221 gcgggtcgtc gaagccaaca tccttttgag cggcatcggg ttcgagagcg gcgggcttgg
    32281 cgcggcgcat tccattcaca acggcttcac cgcgctcgag gaaagccacg cgttttatca
    32341 tggcgagaag gtcgctttcg gcgtcctcgc ggggctgcaa ctcgccgatg cgccgcccgc
    32401 cgaatcggag gaggtctttt ccttctccga gaagatcggc ctgcccacca ctcttgccga
    32461 tctgggattg gggaagcgcg gccgcgccga cttgatgaag gtggccgaga aggcctgcga
    32521 tccggtcgag tgcattcacc atgaagcggg ggtcatcacc cccgataagg ttctcgacgc
    32581 cattctggcc gcggacgcca tcggaagaag ccggaaaggc tcgagtggct gacgcctcgg
    32641 tgttcgggag gctgatcata gacgtcgcgc tacagcaacg aagcgtcggc atggccatcg
    32701 ccgttctggc cggcctctcc ggggtatgga ccgcccgcgc gcagattccc ttcaccaata
    32761 cgattgccgc ttcgcgggcg accatcacga atgtgatggc ggaatacggg atcccgggtt
    32821 gcaccgtggt gctggtcgaa agccagcgcg tggtgtgggc tgaaggcttt ggccttgccg
    32881 acctcgaaac acaagcgccg gtcacgacca ataccgtcat gatgatcggc tccgtgtcca
    32941 agttcttcac cgccatcatg gccgcgcaac tggcggatga gggcgcgctc gatctgaacc
    33001 tgtccgtcac caattacctc cccgacttct ccatgcgcga tcgcttcgcc ggcccgccgg
    33061 aggcctggac cacgcgcaac ctgctggatc atcacgccgg gctgcccggc gacatctaca
    33121 acggttcgtt cggagcgagc gactactggc cgggctatat cgcgtggatg attcattact
    33181 ttcagcgcga ttatccgctc taccccccga atgtcgttgc cagctactgc aacagcgggt
    33241 tcaacctcgt cggcgaaatc gtcacgcgcc acgacggcgt ggattacgcc gtcgccgccg
    33301 agaaccgcat cttcgcgccg ctcgacatgc cgtattcctc ctttttaccc gacaagccgc
    33361 tcgtggccga aaacctggcc accgggcaca gcgccggcca gcccacgccg cccatgatcg
    33421 gcaacatggc cgccaccggc ggcgcctttt cgcgtccgct cgacatggcc cggatcatca
    33481 caatgatcct ggcggacggc gtggccggcg gctcgaatta cctttcgcag gccatcctgg
    33541 acgaaatggg cacgcccgcg aacgcgccgc tcgatctgga catcttcctg caacccggac
    33601 tgggactcga ctccgtggcc gatcatgccc tggcctatgc tggaaacgta tggagcaaaa
    33661 gcggcgacac gggcacgttc tcggccatgc tccaagtcct gcgggaacgg caactgggcg
    33721 ttttcgtcaa catcaacatg agtcattccg ccggcttcac gatctcccgc gccatcctcc
    33781 gggaggcgct gctcgaacgc gacaacctgc ccgatcccgg ctggcccccc atgcccgatc
    33841 cggccgcgac caactggccc tacgccgcgc tgcaggacat cgagggctat tacattaccg
    33901 cggcgggcgt ggaccggttc atcgcggagg cggacggcac gctcacctgc attcacgacg
    33961 cgcagaacaa cacggaggcg accacgtcct ggtatccgca cgtccatggc cggttctatc
    34021 cgccgggcct gcatcacgag cagtttgtct tcaccaaccg ggcgggttgc gacctgatct
    34081 tgcggtatgg cagcagcggc agcgcgcggg atgaaatcgt ctatggcggc tatgccgaag
    34141 gcttgtacgg agtccgctat accccgccgg ccatttccgc cgcatggtcc aaccgcgtcg
    34201 gaacaaagtg gctggcctgc aatctctggc acgacgactg gatgtatgag gccggcgccc
    34261 tccccgcgtt ccttctgacg gcgggccacg gcatcctttc actggccggc ccgcagggcg
    34321 cggtcctgga acccgttgac gacgggctgg cctttgtcgg cggactcctc acgcgcggag
    34381 acagcgcggt gcgcatcgag accaacgccc tgggccgtga gttcctctgg ttcggcggct
    34441 atcgctgcga gcgctacgac gatctgcctg aactgcagaa gggcgtggcg gtttccggcg
    34501 cggcctccgc ccataccaac gccctgttcc gctacgagca cggggccgag ggacaatcca
    34561 tcctgttggc gcttacggaa aacgcgtcca aggccgtcct caccgtgttc aatgccgaca
    34621 tcgagcccat cggaaggggc gagggctgtc tcgcgctcga tctcagctcg gacgcccccc
    34681 tgatcgtcag catcacatcc gccgatccgg tcgagtttga catcaagacc gtggacatca
    34741 cggaagtgcg cgcggccatg cggcggacgt tggatcagta tccgtttgtc cccggcttcg
    34801 gcgtggccgc gcaggaaccc ggcttcccgc ccatcctcct caccgagggc catgcgcgaa
    34861 tcagcccgcc ctcgccggaa ccctccctgc cgctgcaagg gcacgagcag ttccacatcg
    34921 ccagcatctc gaaaacctac acggccgccg cgatcttcct gctccagcag cgcgggttgt
    34981 tgaacatcgc ggactgcgtc accaactacg cgccggaact caacatcccg cgcgccgacg
    35041 aaatcacgct ggagatgctc ttgcagcatc gctccggcct gcccgatgcg aacaacaccg
    35101 gctggttcga cggcaagctc gaaaacaacc cgctcctgga attcaccgtc gaggacatcg
    35161 tggacgtggc ccacgggctc taccccaacc tgctgttcga accgggaacc gcttatcact
    35221 atacggacac gggctacaac atcctcgccc gtgtgatcga aaacgtgacc gcacgaatta
    35281 ccaaggcttc ctggccaacg atgtcctgcg gccgctcgga ctgaccaaca ccttcgcgcc
    35341 ctataaccat caaaccgaag tgccggagcc gtcgatcagc acctacatgt tgatcaaggg
    35401 cgagttccag gatcgttcga tttggaatcc ctcggtcgag ttcggttgcg gcagcatgat
    35461 caccacgctt gaagagttga tggacatggc gcacgccttc ttcatgacca ccaacctgct
    35521 cgacgaaacc acgcacgcgc tcatgatgga acgagtcagc gcaacgggcc tggatcatta
    35581 cgggcgcggc tgcaactatg ccgagggcct cggctggggc cacgacggca cgatgtgggg
    35641 cgcactctcg accgcccgcg tggacacgaa caacggcgtg cgcctcgccg ccgtgatgag
    35701 cggccagtac gaggatgagc gattgatggg ctcctttttg gcgatgcgga gcgcgctggg
    35761 cctgctgaag aacgcgctcg gttacgatgc cggaacagtt ggtcgtcacg aacccgtggt
    35821 gtacccggtc ctcggcgcgg cgcggcaggg gcaccccttc cgctttcagc cgctctcctt
    35881 caattttccc accaactggg cggtgaccgg cctgcccgac ggcctgacct tcgatcccgc
    35941 caccggcgag atcaccgggg ccacgcccat tcgcgggacc cacgccctta ccgtcacggc
    36001 gcagaacgcc tacgggagcg tcgaggtcga attggagctc gaggtccaga ccggctacac
    36061 caacaccatc gccgccgtct ccaaccatat cgtcgccgcc atggtggagg agggcaccat
    36121 cggcgtgtcc atcgcgctcg tggacgacca ggaaatcgtc tgggcgcagg gattcggctt
    36181 cgccgaccgc gaggcgggcc tccccgtcac taccgacacc gtcttccaca tcggctccgt
    36241 ctccaaggcc ttcaccgccg ctctcaatct gcaatatgcc gagcgcggct tgctcgatct
    36301 cgacgccccc ttcactaatt acacccccga catcacgtgg atggaccgct accccgccgc
    36361 ccgcgccatt accgtgaaag acctcttgac ccaccactcc ggcctcccgg gcgacctcat
    36421 ccgcgccggg ttcctgaccc aacccctcgg ccgcggctat ctcgaaacca cccacgacct
    36481 cgcgcaaacc tatcccgtcc tcgaacccgg caccatcaac aactactgca acgtcggctt
    36541 cgtcctcctc gaaggcgtcg ccgaggccgc cgccgccacc gagggcgatc cccgctcctt
    36601 cgccgaactc gccaacgcca ggctgtttga aattctggac atggacgcaa cctccttcaa
    36661 attcgacaag cctgccatct cgaaccatct cgccgtcccc tacatgggtg gcgagcgcat
    36721 gccgcccgaa tacgtcgaaa tctacggcac cggcagcatc tacagccgcc ccgtcgacat
    36781 ggcccagttc atgaaagccc tcttttcaga cacccccctc gtcctgcgcc ccgaaacccg
    36841 cgatctcatg atcgccgacc attccaccaa cgcgcccttt gatgccttcg aatgggtcaa
    36901 aaccggcctt ggctgggaca ccgtctccga tccgcgcctc tcctacgccg gccccgccgt
    36961 ctggaaaaac ggcggcaccc tcgcctacag cgcccagttc ctgttcctgc ccgaaaagaa
    37021 actcggcgtc gccatcgccg ccagctctcc cagcggcatc cccatggccg tcgatgccct
    37081 ggccctccag cacgccctgc tcgaacgcga cggcctgccc atccccacca acgccatcgc
    37141 ctggcctacc aacctcgccg ccgtctcgca ggccgaactc gacgccctcg ccggcgtcta
    37201 tgtcggcagc gccaactatg atcttgtcga atcccagccc ggcttcctct cctatcgccg
    37261 caacgtgccc gatgacggcc tctccctcca aaacctccgc ctccgcagcg acggctggtt
    37321 caggtccgac gacaagcccg accatgccat ttccttcacc aacgcccacg gacgcaacct
    37381 cgtcctgcat cgccagaacc acggcttcta cgatacgccg tccatcctcg ccgaacgctt
    37441 cgctccgccc ctcctccccg ccgcctggtc caaccgcctt ggccggacct ggatcgcccg
    37501 caatgccccc cccgacgact acatacggct cctcggcgtc gcccccacgc tccaattcat
    37561 ctttgaagac aacctcctcc gcgtcaaccc cggcggtatc ctggccgatc gcgtcctcga
    37621 acccgactcc gatgatctcg cgtggatccc cggcctcgtc aaccgcggcg attccgccgt
    37681 ccagatcctc cacctcggcg gcgtcgaaca cgtcctgtat gctggcttcg ttttcggccc
    37741 cgaacccgct gacattcccc tcgccggcac tgcgtccaac accatcgccg ccgaacgctt
    37801 cgccgactgg tacgcaatcc agcccgccgg acctcccgcc cccgtcggtg gcgtggacga
    37861 catccagtac cagctcaccc tctccgatgc gccaaacacc ttcctcctgc gcgtttacga
    37921 agccgacggc gtcacccccg tcgccgaacg caccggcaac ggaacgctcg atttttcttc
    37981 cggcaacacc cctctcctgc tgaacctcca gcccggttcg gacggtcccc caaccggcgc
    38041 ctatcaaatc gacttctccg tccccctcct cgtccgcgcc ctcgcggtgc attcctccac
    38101 cgtcgacatg gtctggcaag gccccgccaa tacgcccgtc accgttgaag ccgccgccca
    38161 ccttgacgcc accaacacct tcgccgtcag gaccaatctc ccgcccgatg cctccctcct
    38221 gcgccagcaa atcctttccc ccgattccgc ctctctacaa ttcttccgcc tctcctcgcc
    38281 ataatccctg cgccctcgcc gatcttcctt cgcatgatta acttcggccc gcgcggtctc
    38341 gtccccgcct cgcgggaggg gtcacgtctc catattcatc aatggatagt caaggccggt
    38401 tgtgggaatc ggaaggcatg gcgcgaccct tgcggatcaa catggcgggc ggctggcgcc
    38461 accgcccctc gcggggacag aagcgggagc ccatctacga tcgagatcgc caggagtttc
    38521 tggaccggct ggaggagatg acgaagcgct acggcgtggg agcccacgca tacgtgctga
    38581 ggccgaacca ctaccatctg ctgatccgca cgcccaaggc gaatgccagc gcggcgatgc
    38641 agcggctgaa caacggctac ggaatgtggt gaaaccgaag gcgagggccg gtgggccacg
    38701 tgttccaggg acggttcaag agcatcgtgg aggaaaaagg gcatgggttg ctagaggtta
    38761 gtctgtacct gcactgcaat ccggtggcgg tgaagcgatt gggataggga aagagggaga
    38821 cggcctcggt tgtgcgggaa acgaggggaa tgggaggacg tcgtcaaggc ggtggagaag
    38881 gggacgggcg agtcctggac gagcttcgta gaccggcacg gggattgggc gttgtgggtg
    38941 gcgcgccggc gggcagggct gacgctgacg gaactgggac ggatggactg ctccgccgtg
    39001 agcgaagcaa cacgctcgct tcgagcgtcg gagcctccgc ctgcccgtcg tccgccgagc
    39061 cagggccgcc gtgtttcaat tgttgaatat ggagacgcga ccctgttctc cgccagaact
    39121 gggaagcctg atctttcgct gccggtcgcc gtgctacggc aggtggaccc gcaggcggta
    39181 cagcttcagc gccgtggcgt ccagcacgtt cgtcatggag atccagccgg tttcggtggt
    39241 caggttggtc aggccttcca ccggtgccca gttctgccac gggtagtccg tgtcgaattc
    39301 cacgtcatac acgcggcccg tagccgccgg ccagctcaat agatggccga tcacgtcgca
    39361 gaaagtctgc gtgacctctt caaacggtga ttcgctattc gtccacacga catcgtagca
    39421 gttggtccca tagacttccg aaatgcgcgc cacgtgcagg aacgacaggc cgtttgtcgg
    39481 atcggtgttc atgacgaact cgtccccggt cggaatggca tccccgtccg ggtcgtcgtt
    39541 caccgcgtcc tcgaagtcgt tggtgatgcc gaattgcgcc agccaccagt gcggcgtcgg
    39601 ccggttagtc gtccagagcg ccgtgaacca ggccgtcacg gatttcgccc cgtccatcac
    39661 cagattcagc gggttggtga actcgtcggg ccccgccacg tcgccggtcc agttggtgaa
    39721 cacaaaatac ggatccggca gggcctcgat gctcaccggt tcgttgttgg tgaaccaccc
    39781 gccgagtcgg tccaccgacc cgttcggccc ggcgccggat tccagccggt actccgtccg
    39841 ccacagccat gtgagcgtgg cggagttgat gaccgtcacc accgcctggg tgccgtcgcc
    39901 atggtccggc gccaggccct cggtagcgac ccatccggcg cagacgtact gcgtggtccc
    39961 ccgcgtgtcg ggcgactgcg cctggttggt gatcacggtc ccttccacgt acgcatggcg
    40021 gcccgtcgtc ggcacggtta tgccgtacgg cgattcgatg tccacggtca cgatggccag
    40081 gacggtgtaa tggttcgtca cgtccggagc aggattccag ttcgcggcgc ccgcctgcga
    40141 ggccacgatg attgcttcgc ccggtccggt gaacgtcagg ttcgtgccgt cgttgatgac
    40201 gcccggatcg ccgccgaccg cgaaggagac cggcaatccg ctcgacgccg aggcgctcaa
    40261 gcccaccgcc gcgtcttcaa cgaaaacact gccgttggtc ggcaggaaat cggtgataat
    40321 ttgatctgct ttcgacacga cgagcgtatc cgcggcggac ccttcgtagt tgacgtcgtt
    40381 gatcgtgccg gtcacggcgt agctgcccgc attggtcggg gcccaggcgt tgccgtcgta
    40441 ggtgaactcc accactagcc ccgccggatc gctcgtggcc gttacgctct tcgctgtgcc
    40501 gtcgtaggcc tgggcgaggt tctgcaagaa cacgcccgcc acgcccttcg acaccaccag
    40561 cgtgtccgcg acgctgcctt ggtaattgat gtcgttgacc gtgcccgtca cggcgtagct
    40621 gccgacgttg gtcggcgccc aggcgttgcc gtcgtaggtg aactcgaccg tcagccccgc
    40681 cggcatcgtc gtcgccgtca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40801 nncgacgccg aaccctcgta gttcgcgtca ttgaccgtgc ccgtcaccgc gtagctgccc
    40861 gcgtttgtcg gcgcccaagc atggccgtcg taggtgaact cgaccgtcag ccccgccggc
    40921 atcgtcgtcg ccgtcacgct cttcgccgcg ccgtcgtagg tctgggccag gtccagcagg
    40981 tacacctgcg ccgccgcttt gcccacggtg tagacattgg tcacttcggc cgccgggttc
    41041 cagttcgcgt cgcccgcctg cgaggccacc acgagcacat cccccgcgcc ggcaaacgcc
    41101 atgttcgtcc cgtcactaat gacgcccggt ccgccgttcg tcgcgaagct cacggccagc
    41161 ccgctcgacg ccgcggccga gagccccgcc gcgtcggtcg tcacgaacac gctgccgttc
    41221 gtcggcagga agtccgcgat ggtctggccc gccttgttaa tcgtcagcgt atccgacgcc
    41281 gaaccctcgt agttcgcgtc attgaccgtg cccgtcaccg cgtagctgtc cgccgccgtc
    41341 ggcgcggttg ccgacccatc gtacgtgaac tccaccgtca agcccgccgg catcgtcgtc
    41401 gctgttacgc tcttcgccgc gccgtcgtag gtctgggtca ggtccagcaa atagacctcg
    41461 gccacagcct tggaggccac caccgcgccc gtcacggacg cttcaaagta caggtcatcc
    41521 tgcgccttgg tggcgcgaat ctcgatggtt ccgctgctgt tcgtcaccgc cagattcgtg
    41581 tcatccacca ggattcccgg accgctcaag acctcgaagc tcgccgcgcc cgtgcccgag
    41641 ccgccggaga cggtgagcgc gttggtcgtg agatacgcct ggggactcgc cgggttgaac
    41701 accagcgagg cctggtgttc gcgcagatac tcgaaggcgt tgctcttcac cgtctcgccg
    41761 aaactcgttg agaacacccg cacgtcgcca acgccggcgg cggccgcctg cccggcgatg
    41821 acgacgattt gcgttgcgct ctggctctga atggccgcgc tcatcccgca caacgtcacg
    41881 ttggtcgcgt cggtaccatt gcataagttc gtgccgctga tgaccacttc gtagccgccg
    41941 gtccaggatc cgctttcggg caataccccg cttgcgccag gaaggttggt ctggccagcc
    42001 tggagggtct ggccgtccac gctcacgaag gccaaccgcc ggacggagaa agtatccgta
    42061 tcgttacggg aagcctccac ccagccataa cggtaatcag cctgcccgtc gaactggaag
    42121 cggatgccga aatacccata gatgccatcc gtccattcaa tcatgttgaa cgacatggcg
    42181 tccacgttcc accaactcag cgtgccgttg atgtccgatc cggagtccag attgtccacc
    42241 gtggttccgg taaagacgac ctgcggcaac aacgcctggg tccagttcca gcgctcgagt
    42301 gtctgcgtca tcatgtcgga attgagcacg agcacgttat cacccgccaa ggcattggag
    42361 atgtagccgt cacccatcag atcgcccagg aagatgctcc acccggtcag gggcgcggag
    42421 gaaatatcca ccaccttgac gccttggccg cttccgatcc ttcccgccgg attgtacgtg
    42481 tacgcccccg caaaggtgat gtcgccgtgt tcctcggtct gcaccacgat gtccaccgtg
    42541 ccggtcgtgc ccagcgccgg agcggtgatc agcaccacgt tcgccgcctg gggaaggaag
    42601 ggagcggaca ctcctcccac cgtcacgttg gtgatggtgc cgaagacgcc gttgatgatc
    42661 gcgatcgtat tcccacccgc gaacggcccg atgttgggag ccgcctggaa tcccgcgccc
    42721 gtcagaccga aggcatagct ggggagaggg ctgttgttgt ccacatagca ggtcggcgtg
    42781 aacgacccga cattcgaagc cgtaaagacc accgggaagt tgctcgccgt cccgggttcg
    42841 accgtcagcc ccaccagcgc cgatacatta aagtatgccg cgccctcgcc gtcgttcgtg
    42901 gcggcggtga tcagcaacgc ctcgttgcca aagttggtca ccgtgaagat gtttgtcacc
    42961 accgcgccga gcgcggccgg agcgaaagcc ggaccggaaa tcccgatgcc cgaagctgtg
    43021 taggtgaagc cattcgactt caccgtctcg ccctggctcg tcgagaacac gcgcacgtcg
    43081 ccgaggatgg cccccgcggt cgcccccgcc gtcaccacga tctgcgtcgc gctctggctt
    43141 tggatggccg ccgacacccc gcatagcgtc acgttggtga tgtcgccgcc gttgccgaga
    43201 tgaatgccgt agatcgtgac cacatccccg ctactcccgg acgtcggcaa cacgccgccg
    43261 ctgtagcctc ccaagccctt ccagacattg gtatgcacgg tggatcccgt atcgccgccg
    43321 acaacataca tattcgttcc caaagcgccg cagccggcgg acgaacgcgc ctgcggaagt
    43381 gagacaactt ccatccaggt ttcgccatca tatcggaacg tgttggacga ggcgttgctg
    43441 ccggtccatc cggagatcgc gtagatgaac ccgccgaccg tatatgtcac catactgcaa
    43501 agcgattgcg gcagtcctgc tgtttcggtc caattagttc catcgaaacg atagacattg
    43561 gtataggtgg tgccggcctg tttcccgccg atggaataga gttgatcatc cagcacggcg
    43621 gccgtattgt agctacgcgt cgacggcaac cctttggtct cggtccagga aaccccgtcg
    43681 aaccggtaaa cgttggtgca cgcagtgccg ccgacctccc cgcccatgac atacagggca
    43741 tttcgccagt aagccaaagc aatcgcagtg cggtctgccg gcaatccggc gacttcactc
    43801 cacacgccgc cggaatacat gtaaacattg gttctcgcgc tagaattata gctgccgcca
    43861 acaacgtaca ggttcccgtt cagctcggcc gcccggcaat ccacgcgagg ctgcggcaag
    43921 ccggccacct cactccaggt ggcgccgtcg aagcaagtga cattggtaac cgtcgaggaa
    43981 gcgttccgtc cgccagcaac atacagctta ttgctcacca ccccggcgcc aaatgccgct
    44041 tgtccgtagg gcaggccgac aacttcgacc caaccggtta aagatccggg ccgcccgatc
    44101 aagcccgccg gattcaccat gtacgccccc gccagggtga tgtcgccgcc atccgtctgg
    44161 atcacgacat ccttcacccc cgccgatccc gtcgcgggca cgacaatcgt cacccagtca
    44221 ctgcccgagg cctggacgga cgccgcgacc ccgcccacca gcacgttcgt gatcgtgccg
    44281 aagtggccgt tcgtaatagt gatcgtgttt ccgccggcgt aaggaccggt ccacatgtcg
    44341 agcgcatata catggagggt gttggtcacc tccggcgcgg ggttccacgt cgcgtcgccc
    44401 gcttgcgagg cgacgacgag cacctcgccc gcgccggtga acgagaggtt cgtgccgtcg
    44461 ctgatgacgc cgggtccgcc gttcgtcacg aagctcaccg ccagcccgct cgacgccgtg
    44521 gccgccagcc ccgccacggc gttcgtcggc tgcgcgccga tgggcgggaa cgtgatcgtc
    44581 tgatcctgct tgccggactg acccgccagg ttcagcacga acgggttgtc gccgcccccg
    44641 tcgtgcgcga tatgcaccgc cacggtggcc gttccgcccg cggacggcgc gaacgccacg
    44701 acgaaatccg ccgtgtcgcc cgcattgacg gacgccggac cgctcaccac caggaagcct
    44761 tccgagccgt tggtggtcca tccggtcagc gtgagcgtct cattgccgcc gttggtgatg
    44821 gccaacgtgt tggtgaccgc cccgccgggc aagaccaacc caaaatccgt gcccttggac
    44881 tcggaggccg cttcgtcgga agcgatctcc gccccattcg tcccccgcag gcccaaaaag
    44941 gcgccgttat aggtgaaggc attcgacttc accgtttcgc cgtaactttc ggaatagacg
    45001 cggacatcgc caagccccgc ggcccatgaa acgcccgcga ccaccaccac ctgcgtcgcg
    45061 gactggctga ccatcgaggc gccactcacg ccgcacagcg tcacattcgt gatgtcgctg
    45121 ccgctgccga gattgctgcc cgtgataacc acttcatagc cgccggtgac ggagccggag
    45181 gatggttcca cgccggagga agccacgtcc tccgtaccat actttgccac ataccacgta
    45241 tccgtgcccg cgccgtcatc ctggtaggcg atcctgaaga agccgccggc gcacagatcg
    45301 ctgttgttcg tattgaccgt caaggcgagc gccgtgtcgc tgaggccgca ccccatgttg
    45361 gtccagtgcg tcccgttcca catggcgatg cgcttggtgg cgagtttcgg ggcggacttg
    45421 tccggttcgg tcgagaggta ggtgttcgtg aacgcaccgg ccgcgtagag cgtgccgttc
    45481 gtgtcaacgg ccagggcata aacccagttg ttgaaacccg agccgacgtt ggtgagggac
    45541 tgtgaaaccg gatcccattt cacgacatag ttcgcaagct gcttgttgcc cgcgctatcg
    45601 gtgtagttcg tgaaccagcc gccgatgtag agttcgccgc cggccgggtg aagcgccatg
    45661 caggtcgcgc ggttacccag accccgcgcc atgttggtcc aacgcgtgcc attccattgg
    45721 gcaatgaaat tgagatgaac cgattcgttg gtcggataat gattggtgaa cgagcctccg
    45781 acgtacaggt gatcgcggtc ccggtcatag gccgccgtca ggggcacgtt gcggaagccc
    45841 ccctgcatgt tcgtccagcc gacgccgtcg tattgggcga cgtaattcac actgccgacc
    45901 cggccatcgg tgttggtgaa atagccgacg gcgacgacgg tattgctctt gtagggctcc
    45961 atccagttca cgtagccgtt gatggtcggg gtgaaaccca agcccatcct gttcgtatcg
    46021 ccggcagccg gcgggcgtcc catggggttc cagttcgtgc cgtcccaccg gcccaccgcc
    46081 aaaccgccga tgccgccgat gttcgtgaaa tagccgcccg cgtacacgcc gtacgccccc
    46141 cccttgatgc aattcacatt ggcggccttc ggcaccccga tccccatgct gttccaattc
    46201 gtgccgtcgt accgtgcgac gcgggcgcaa ttggacccgc cggcattggt gaacgcgccg
    46261 ctgatataga ggttgttgcc gtagtaattc atcccgtaaa tcgtgttgtt cgcgccgatg
    46321 gcgttcgttt ggccgggaac gagctctttc ccgacggcgc tccacctctt cacggacacg
    46381 ctgctcccga tctcgcccgc cgcgttgacg gtgtaagccg ccgccagcgt cgtgtcgcca
    46441 tggtccgagg tctggatgac aatgtccttc acgcccgccg tgccgatgaa cggcaccgtg
    46501 atcgtcaccc agttggcgcc ggaggctttg atcgtcgcgg ccgccccgcc caccagcttg
    46561 gcgccggagg ctttgatcgt cgcggccacc ccgccgacca gcacgttcgt gatcgttccg
    46621 aaataaccat tggaaatcgt gatcgtgttg ccgccggcga aggcgcccgc ggaagcgctc
    46681 agggcataca cgctcacgat gttggtcgcc tccgccgccg cctcgtagtt ggcgttgccc
    46741 gcctgcgagg cgaccacgat cacatcgccc gctccgctaa aggtcaggtt cgtgccttcg
    46801 gcgatcgcgc ccggcccgtc ggtcacggca aaggtcaccg gctcgccgga tccgccgccg
    46861 gtcgcggcca gaccgaccgc gttggtgacg tgttgccacc cgatggccgg gaaggtgatc
    46921 gtctgcgcgg ccttggaaac ggtcacggtc acgctcaagg tctgcggact gttcgtcgca
    46981 ttcgcggagg caatggccag attcgccgtg tacacgccgg ccgccatccc ggagctgtcc
    47041 acggacgccg tgacctccgt gctgctcccg gtatctaccg tgccgccggg cggaacgcac
    47101 gacagccagg ccgccgaaac cgttccggtc caggtaaagg ccgtcgagcc gacgttgctc
    47161 acggcaaagg cctgcccggc gggactcgcg ccgccgtaga cgcccgcaaa cgccagcgag
    47221 gacggactca acgcgatgcc gccgttggtg ctctcgggcg tgtcgtcaag gatcatggaa
    47281 atgatttgca gatcgtccac gcagacaccg taggcatact cgccgatacc ctcgaaggcg
    47341 aagaccatgt tcgtgcccaa gacattggtg atcgtgagtg atcgttgtgt ccaattggtt
    47401 acaccggccg tgtagtaggc cagcacctgc cagcccccgg cgcccgcgcg cccgcccttg
    47461 ttggtccagg cttctggtgt gatgttggta tgcgccagca cgtaaaggta atcctggtcc
    47521 gccggccaag gtacctggca atgccagaag gtcatcgtcg gcatgtactg ggctcccgac
    47581 aggtccagcg gcggcgtgta cagccgcgtc acgttgccat aggaagcggc gccataccga
    47641 gcgaagttgc tgaaggtacc cgttacggga ttcgtccagg cgccctcccg ggcgcgcggc
    47701 gggtggccgt taatcccgtt cgtcgcccgc acccagtcgg tgttgttcga catgatcagc
    47761 ttcgtccagc cgcgggtcgt cggccagccg cccgccggcg tgttgctcgc ggtccaggcc
    47821 gtctcaaagt cctgccacag gttggtgacc tggccaaaga ccggcgccgc cgtgttgttg
    47881 ccgaagacaa tcatgtcatc cacgcaggtg ccataggcat actcgccgat gccctcgaag
    47941 gcgaaaatca tgttcgtgcc caggacattg gtgatcgcga gggagtgttg cgtccagttg
    48001 gttaagccag ccgtgtaata ggccagcacc tgccagcccc cggcgcccgc gcgcgccccc
    48061 cgattactcc aggtttccgg cgtgatattg gtatgcacca gcacgtatag gtaatcctgg
    48121 tcggccggcc aaggcacctg gcaatgccag aacgccatcg tcggcgtgcc ttggaacccc
    48181 gtgaggtcca ccggcggcgt gtagagccgc gtcacgttgc cataggaagc ggcgccatac
    48241 cgagcaaagt tgctgaacgt gcccgttccg ggattggacc aggcgccctc ccgggcgcgc
    48301 ggcgggtggc cattaatccc gttggtcgcc cgcacccagt cggtgttgtt cgacatgatc
    48361 agcttggacc agccgcgggt cgtcggccag ccgcccgccg gcgtgttgct cgcggtccag
    48421 gccgtctcga agtcctgcca caggacagcc ccatgcccgg ccggcaggtt ggtcggcgag
    48481 atgttggtcg tcagcacttg ccccagggcg gacaccgcga ccgccaggta gagcacggcg
    48541 aagagacccc cgcaggccca gccgatgctc atccggcgca gaatgatttt tcctatgcct
    48601 ggaaaaaagc cgagcatttt ttccaagcat tggaaaaacg aggagcgttg tttccaaaca
    48661 tgggggaaaa aacgcagggg gcgttcgagg gagtgcatgc gaggagaatc aatcccggag
    48721 tccgtacgtg ctgtttttgt catggcaacc ctctcgtcca tgttcggctt tgcaaatttc
    48781 aatgatcacg ggaggcagcg gccgcttatg gcgactgctc ttcttttttg gtcggagtcg
    48841 aaacgatcgg aaccgcatcc gcgacataac tcatgcgctg tatgcgtctt cttcatcggc
    48901 gaatcacttg gcacacggga tccctaaagc agtaatcgtg ccgtttgggg caggagcaac
    48961 acaacatgct gaataataaa ttattgcgta tgcgagcgcg cgattatttg tctcaatatg
    49021 agacgcccgt ctcaattcga cacgcattct gaagtggctc cgcgtcatag aggcgtccct
    49081 ctcttttttc gcgccacatc gcgcccgccg aggagatcgc tttctccgaa aggggcggcg
    49141 cgatgacaat ggcggcatcg ggatggctat ccctcagttc cggcagccag ttcaggccgt
    49201 tgccgttcgg cagttccaag tcgagcagga ttccgtcaaa cacgttccgg ctgaccgctt
    49261 cgcgcgccgg cgccgggccg cgctcgttgt ccgccttgac catttcccgc cgccgttacg
    49321 gaatgaccca cgaccttggc cctgctgttt ccgtccgcgg tcggagcctt ctgccggacg
    49381 cctggattct tccttgacgg tcgcgcgtgt gaaatctacc ccttgtgcat gaacatggag
    49441 ccccgtgccg ccctcacccg cccagccgcg cggatcctct ggatcgcccg aggggtatgc
    49501 ctgctggccc tggcgggcac gttgctgggc ctgctggcct ggtgcaggct caaggggtgg
    49561 tcgcacgacg aggtcgagaa tatccacgcc ggctggctgg tatataaagg gcaggtaccc
    49621 tacgcggatt tcttccacca caagccgcct ttttactggt tcttcatcag cttgtactac
    49681 cgcctggccg gggagaacct ggccgtcatg ctctgggcgc ggcactggat gctggccacc
    49741 ttcgtcctgg cggcgctgtt cacgtttctc gtggcgcggg aacttttcga tgagtgggcc
    49801 ggttgggcgg ccgcgggcat cctggtttgc aacgccctga tccactaccc ggccatcgag
    49861 ccgcgcgagc aggggtggat gctggcgttt ctcatgatgg gattgtacgt ctatctgcgg
    49921 gcctggcgtc gcgcgtttcg ccctcgcgac ggccttctcg ccgggctcct gctgggcgcg
    49981 gcctacgcgc tccatccgcg ggccgggtat ccgatcctgg ccctggccct cggcacgggt
    50041 ttccgttgcc agttccagac aggctggcgc gtcgtcgggg aacgcaaggc ggccctggct
    50101 ctattggcct tggccttcct gggcgtggcg gccattccgt atatcctgta cgggaaggag
    50161 tttcaccacc tgctctaccg cgtcagcctg gcggtcgcgc cggaagaccg gttcccgccg
    50221 tggggcctgc tgaagaatct ttatttcagc acctacgcca tcatgccctt cgcgctgctg
    50281 ggcttggccg cctgcgcggc ggacctggcg aaaaagggcg agcggcaatg ggcggctttc
    50341 tgcgcgctcg tgttcttcac gctggccaac ctgcagatca tcagcaaccc ccgcgccttc
    50401 gtgcaggcct tctacagcgc cggccccttc gtggccctgt tgtgcgcggc cgccgtgcac
    50461 tggatcgggc gacgcctgac cgcggcccaa acagccctcg gcgttgcgtg tctcgtgacg
    50521 ggagggttcc taagcaacgt gaacccctcc tactggaccg cgccggacga atacgcccag
    50581 gccgagcggc gggcgcggta tctcctggag atgatcccgc cgggggagcg atatctcggc
    50641 atggacgcgc accccatttt ccggctggac ggcagccgct actggttcga catgaaccac
    50701 gtcctgcgct cgctccagct cctggacccg acgttccggc atgacgtggt tcaagaactg
    50761 gagcagaccc ggccgtactt gctgggtgaa gacgtgttcg ggaaggccga tttcgatccc
    50821 gttcagaaac cgcgcctgga aacctacgtg tcggagcgct accagcggct gcccaacgcg
    50881 ccttttttca tccggaaaga cgccctatcc ccggaccggg ctccgtgaag attctcgttc
    50941 tcaatccgcc ggcgcacgag ggccgtccat acatccgcga aggccgatgc atgcagattg
    51001 gctcgtcgtg ggccacgctc tggatgccgc tgaccctggc ctacgtggcc gccgtgctgc
    51061 gcgaggatgg acacgacgtc cgcctggcgg atgccgtcgc ggatcgcctg gacctctccg
    51121 gggtcctggc cctgcgcgac gagttcaagc cggacctggt gtttctcaac acggcctttc
    51181 cctccatcga aggcgatcgc gccgcggccg ccgccttgaa gcgccgcgcg cccggcgttc
    51241 ccatcgtcct gctcggcctg ttcccgaccc tgctggaaga caaggccctg gactacatcg
    51301 ccggcgcgga tttcgggctg atcggcgagc ccgagtgggc ggcgcgcgaa ttggccgccg
    51361 cgctggccga gggcggcgat aggaccggcg tcaagggctt ggcctggcgc cggggggggc
    51421 agatcgtggt caatgcgccg caggacctcc ggagccacga tctcgatgcg ttgccgttcc
    51481 cggcgcgcga cctgctgcac aacgacgcgt accgcctgcc gatcaacggc gagcgcttta
    51541 ccctgctgaa catcgggcgc gggtgcccgt atccctgcat tttctgcatc gcgccggtct
    51601 actatggcgc gccgttccgc aagcggatcg tatcgcgcat cggcgatgaa atccaagaat
    51661 gcgtggaacg gcacggcctc cgccatttcc tgttctgggg cgaatcgttc acgacggacg
    51721 cggcctacgc cgaggccatc tgcgaggaga tcatccggcg gggcttccgg gtggtctggt
    51781 ccaccacgtc cagggtggac acgctcaccc cgcgcctgct gggcaaaatg aagcaggccg
    51841 gctgcgccct gttgggcctg ggcattgaat ccgccgacga aaccatcctg gcccattccg
    51901 ggaagaagac gcgccgcgag gatatcaccg gcgcgattcg aatggttcat gacgccggca
    51961 tcaagacgat gggccatttc atcttcgggc tcccgggcga aacccgggag acggccgggg
    52021 aaaccatccg cttcgcgctt cgcagcgggt tgacctacgc ccagttctac tgcgcgctgc
    52081 cctatcccaa aacccggctc ggggaactgg ccgccgagcg cggctggatt acgagcggcg
    52141 attattcctc gtacgacttc accttttcga tcctgcgcaa cgagtccctg accgcccggg
    52201 aggtcaaggc cttccgcgac cgcgcgtacc gacgcttcta tttccggccg cgtatgctct
    52261 tccgggtgct ccgggaggtc cagtccgtcc ggggcctgct ggcctcgatg gattttttga
    52321 aatggatacg caaaaaatga tggccgggac atattgaaag gacatggcca tgtctcgcaa
    52381 aaaaaagaag gctctgaaaa caggcgcgca ggaagatgcc cctgcggcgc ccaaggtcgt
    52441 tccggaaccg gacggtccga aacgcatggc cttgggaaaa acaggcgacg cgacctcgcc
    52501 gtccttcggc cttgccggat ggaaagccgt ggccctgctg ttcggcctgg ccctggtcgc
    52561 gcgcctgtgg gccctcggcc gcgccgactt gtggtgcgat gaaatcctct tcgtcgggcg
    52621 ctcgcatgcg cccaagtcgc ccctgcgcct gctggccgtc tgctggcgcg agtttctcct
    52681 ggtcacgcac ctgcccctgc cggaaatcct gcagaacctc ttcctgtggc tgtgcctgcc
    52741 cttcgatccg gacttgatct acaacaacgg cctgcaacgc ctgccggccg ccctctgggg
    52801 cgccggcagc gtggcgctcc tgttccgtct tggcgccaag atcgtccgcc cagccacagc
    52861 ctgggtggcg gccttgtcca tgtgcttctt cttctacccc gtctattact cccgcgaggt
    52921 gtactactac gccccgctca tcttctttgc cgcggcggcg ttcaacctct atgccgacct
    52981 gttgatccga aggcctgtaa cacgacgcgc cgccgtggcg cttgccgtgt gcctgtccgc
    53041 catggccctt tcccatttga ccggtattct gctggtcttc ctgctcttcc tgtttcatct
    53101 cggctggctg ggctgctcca ggatccggcg cgcgaatccg ccgcccgatt cgccgccggc
    53161 gccgcccgcc cgaagcacgc tctggttcct cccggccttg ctgctcgccc tgccgttcgt
    53221 gctccggttg gcgcttcagc cggtggagcc gctcatggaa ggatcgcccc tgtccgttcc
    53281 ccggatgctc ttgtacgtgc tcggcaagtt caccctgggg acggcgcggc cctgggggat
    53341 cctgggcgtc gggatcatgg tcctgggttt cggggaactg ctggcgaaag ggccgcagcg
    53401 cgaagcgcgc cgctcgctgg gcttgttcac gctggccggc ttcctgatcc tggccgtctg
    53461 gcagacgcgc acccagttcg cggtccgtta cttcactgtc ctgctgcccc tgttcatgct
    53521 gatctgggca gccggcctcg aaacgatcgc gcggtccgcc gcccgcctcg gccgggtcgg
    53581 ggcctccggc gccgagcgaa tgaccctggg cctgggcgcg gtgttcatcc tggcgcatct
    53641 cgcgttcttc ctcccgcccc tctaccagct gcgcgccaag ggccgggact acggcgggct
    53701 ggcgcgctgg ctgacggaaa acctgcggcc cgggaccccc tacttcttcg aatatggcgg
    53761 gtgggactcg aggttcgtcc ccgtctattt ccccaccctc gggctggccc acgcgatcac
    53821 ggaaacagga tacgggccgg aattccatgc cttcctccgc gagcagcaga ccaacctggt
    53881 cgcccgcttc ccggaatccg ccggtatcga agccgcgggc gccgaatggc cgccgctttc
    53941 gcgcttctac cggcgcaagc acgaactgcg gaacgagggg attatccgcc tgatacggaa
    54001 gggcatctgg ttcgagcttc cgctgggcga ccgaaccccg ggcgacctga acatctggta
    54061 caatacccgg gatgacgccg cggacctcgc ccgcgaggcc ggcgcgccgg cctacttcga
    54121 cttcgagggc gccgagtgcg cacagatggc gcaggatgtg tatgtgcatg ccgcgcaagg
    54181 caaccggatg accgtgcgcg tggtcaacct ggcccccgcc ccgcagcgcg ggcgctttcg
    54241 cctcgaaggg gccctgaaca cctcgcaagc cctttgtcct ctctatgtca cctggcaaaa
    54301 ccagttgctc ctgcagacct cttgcgtgca acgcactccg tggatcctgg aaaccccgcc
    54361 cgtggaacta cccgccgggg aacacgaact ggtgttgcgg gcgggctccc gggacgaggc
    54421 ccccgtgctg aacgtggccg tcctgcaggc ggacttcctg ccggtgccgt gagcgcgacc
    54481 atgccggccg gaaaacagac gggcatcagc gtcgtcatcc cgtgcctcga cgagcaggcg
    54541 ggcatcggcg ccgtggtgcg ggacgccctg cgggggatcc gggactccgg gctgccgggg
    54601 gaagtcctcg tggtggataa cggcagcacg gacggctcgg cggacatcgc gcagcaggcc
    54661 ggcgcgaggg tggtgcgcga gccggtccgg ggatacgggg ccgccctccg gcgcggaatg
    54721 gccgaggcgc agcaggcctt cctggtcatg ggagacggcg accgctccta tgacctggcc
    54781 gatatccccc gcctcgtgga gcccctttgg agaaacgagg ccgatctggt catgggggac
    54841 cggatgcgcg atatccggcc gggggccatg ccgtggctgc accgccggct cgggaacccc
    54901 ctgttctccc tgctggtccg ggtcctgttc cgcaacaaca ccgtccacga cgtgcattgc
    54961 gggctgcgcg ccatccggaa ggaatcctat accgcgctac agtgcgccac cacgggcatg
    55021 gaatttgcca gcgaaatggt ggtccgggct tttcgcgcga agctccgcat ccaggaaatc
    55081 gccgtggtct accatccccg cgtgggcgtg tccaaactgc ggtccttccg ggacggatgc
    55141 cgtcacctgc ggttcgtgat aagggaagcc ttcctcgcca aaccataacg gcgcgctttt
    55201 catccccgcc aacgggatgg cacagccgga aaggccattg tcgttcgcca tggcgcgtcg
    55261 cgggcggccg actgagcgca ggaaaaccgt caggcaggtt tccggatagg ttcttagcag
    55321 agatcggaaa tcgtaagacg acgtgaccgc cccccgattc cggacccttt tcagtgagag
    55381 tcgtggcccg ttgttgaccc tctttccagc gctacggccg ccgttgtggc ctctgcgctg
    55441 ccgctctttt tatataaaat ggcgggccgc gccatgccgc atgatgtcct tgcacgcttt
    55501 ccacttttct aagacacgcg atgcctcacg aatcccgtcg aacgcattcc ggtcacacac
    55561 caacccgcgg cggcttccct ccgggcagaa ggaaacgccg agctttccca tgtccaattg
    55621 ccacttgtca cccctccggc gtattggtag cttgtgccgc agttctttgt gatttctcgc
    55681 tgtcatatag ccgttgtaac gttgagatcc tcttttctaa caatactgtc acaagacgcg
    55741 ttttttaaac gcgcaataaa acacaataat caacgcaact agcgtgattg caacaagtgc
    55801 ggagaggtgc cagagcggtt gattgggcac gcctggagag cgtgtttacc ccaaaagggt
    55861 aacgtgggtt cgaatcccac cctctccgcc agttttgatt tttccgattt tccgcgacga
    55921 ccgaccacgg gaccgggaag
//
LOCUS       DAOOTK010000009        51734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_514_length_51734_cov_6.036553, whole genome shotgun sequence.
ACCESSION   DAOOTK010000009 DAOOTK010000000
VERSION     DAOOTK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 51734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51734
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_514_length_51734_cov_6.036553"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PK634_02215"
     CDS             <1..804
                     /locus_tag="PK634_02215"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96582.1"
                     /translation="ERGGGAYTYTDSGAPVVESCTFARNAGAEAGGIYLNGSAVLLNS
                     IAWANSASSDSNIVLFGSGQDVQYSTTGPAVAGTGNSAANPAFVDAVSDNFRLGDASP
                     CIDAGTNQAWMAGALELDARPRVAHNRVDQGAYEAILTAWDTDGNGLPDWWEWQYEKV
                     LTGTDPDGDPDGDHMPNRDEYPAGSHPGDGDSFLGLAAPWAGPAADSIVLSWRGVTGI
                     SYRVEQSTNLAQGFTATLFTNIPGVSPYNSRTDSVAGTATRFYRVRLEP"
     gene            complement(806..3319)
                     /locus_tag="PK634_02220"
     CDS             complement(806..3319)
                     /locus_tag="PK634_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOW96583.1"
                     /translation="MVSIPFACRRWILGLGAFAGAACAAEVPVILTWSEDPQTTVTVF
                     WQRDEPGVGTVRYGLTSNYTDEARDAGGVRRHRITLRGLEPGTVYHYSVSSTDGFAAG
                     DRTFRTAPVATDTLHVVVYGDLQGGGEPGGCRGVASRVAAWAPDLVIQMGDLSDEEYG
                     GAGYAPWALFLNVATDVLDRTVFMPTPGNHDYPESGSSLFWPFFSLPQRDIPGSPYSF
                     RAGPVHFQALDSDVGIEAQTNWLMRDLQAAAYDTNVLWIIPYFHRPPYSWGEHEGDEF
                     IRTNWCPLFTLYGGDLVFNGHNHNYQRTVPIRDITYVVAAGGGASLYDTVLDPGAHAY
                     ATTCYHFVSLQITGSVLRYRAVRSDGLVFEDLTWTNSRRKVRVEPAFPARGGTAKTLY
                     DAAQGPLFYASPVWIHLGVDEFGSALVDTSMTWNASSGFWEYTFTVPASTSNRIAFAF
                     HDAAVTNWDNNYGYNWQALLDDMPYEPPTSPPPRLVAAGTPAITGDPAAQNNTGDAFD
                     FSTNGGPLEVRDGSGFGDLGRLYFNHDGSNLYVGGIGADLGGSNNVFVLFLGLDTLTD
                     NAENLWHKDGAPQSLDQLHNLGFVEPMDLAIVFGDEYGDGPAYATFIYGGYDFGQGIY
                     YIGTNTSFFVPVPDARLSQFDGTGTTACVTEDNDGDRRTDRWEASLPWAALNVSGGAT
                     SLTWLLVSGVIASDSTNGNDRYLSATHIGRRAYGTVDEYGNYGCEFVTMEPVKIALEH
                     GDEDGDGMKNLSEFVAGTRLDDAASLLRAKAEEAAADRFVLRWSSESNRTYTLYQTSN
                     LISGFSELAANITSTPPSNIHTAKIGSAQSGFYRVRVAP"
     gene            complement(3346..3546)
                     /locus_tag="PK634_02225"
     CDS             complement(3346..3546)
                     /locus_tag="PK634_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96584.1"
                     /translation="MKDVLLCLAAFLLLTWVIYLKAQIVKLLQQNERMAALLKLKLDK
                     DKAGMEVGGDDQVIGKKPGWMK"
     gene            3648..5042
                     /gene="selA"
                     /locus_tag="PK634_02230"
     CDS             3648..5042
                     /gene="selA"
                     /locus_tag="PK634_02230"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOW96585.1"
                     /translation="MNKDAGDASPLRALPSVDAVLNTARARALAEQHGRARVSGAVRA
                     AVESRRQAIRGGEAARPDAESLAEAAAELLAADRPNLRRVVNATGIVLHTGLGRAVLP
                     GDTLAALAEAVGNCNVQMDLETGERTRREAVVLDLFRRVTGAEDAAVVNNNAAATLLV
                     LAALSRGREAVVSRGELIEIGGSFRLPDIMAESGALLREVGTTNKTHLRDYEKAIGPG
                     TGLLLKVHPSNYRVEGFVAETALREIAALGRTRSVPVIFDLGSGNLVDLRKYGLEGEP
                     TVQDGFRDGADLVLFSTDKLIGGPQGGAVVGRTDLVTAVRKHPLYRAFRVDKLRLAAL
                     EHALRLFLEPDKLEARHPTYFMLARKPDELRRTAEELRAGIAKRQPAWQATVVEEESK
                     LGGGALPTSPLPTFAVSLRAEGLSADRLALALRRADPPVIGRIRDDLVLLDVRTILPG
                     ETEDVLRAIERLAP"
     gene            5039..6946
                     /gene="selB"
                     /locus_tag="PK634_02235"
     CDS             5039..6946
                     /gene="selB"
                     /locus_tag="PK634_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOW96586.1"
                     /translation="MSHPPVIHVTIGTAGHIDHGKTALVKLLTGCDTDRLPEEKARGM
                     TIDLGFATCAMPDGRRVGIVDVPGHERFIHNMVAGATGIDVVLLVVAADDGVMPQTVE
                     HFHIVRLLGIRRGLVAITKSDLVEPARVAEVEAQVRALAAGSFLENAPMRPVSSKTGA
                     GFDAFYDAFAEVVAATVEHRADGPFRMNIERSFVLKGLGVIVSGIPRSGGVRIGDELE
                     LLPAGRRKKVRALQVYGADAEEAFAGECVALRLSDLDREEAGRGRVLATPGYFQPTRF
                     FNAKFLHLPDAGSALKPRTAIRFHVGTSDAPGYLVLPTLDPLPPGGECFVQVQLAEPV
                     IAAPGDPYVARALSPARTLGGGHLVGTDEQKMRRSRGDWVGTREEEEQALHDPSAALL
                     HALRAAGPAALKAEEAAHRALVAGDAARTELDRLVQAGDAVRLGDRYASADSLRQLAE
                     DLAAALKRLHEAQPKEPGFTRADLRRGLTAEPELVEQALKSLCEAGRIRIRGELVGEA
                     GRSVVDESGAALLAVYRETGFQSPRPDELPARLGWPLARAEAALKPLLQAGELVRLSE
                     KVILHPEHLEASRVKLIDYLSRNDRVDAVKFKEVLGTSKKYAIAILEWWDRKGLTRRI
                     GDERILARKPG"
     gene            7055..8260
                     /gene="selD"
                     /locus_tag="PK634_02240"
     CDS             7055..8260
                     /gene="selD"
                     /locus_tag="PK634_02240"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOW96587.1"
                     /translation="MTRSDINAEKRRQVMGRSIKLGHCVCNPKLPCPCPTFKEKDTCL
                     CAGEHVPADPAAPAARLTEHVRSAGCSGKISQKDLHAVLSRLPKIDDPRVLVGLSTGD
                     DAGVYELTPDLLLVQTVDVFTPVVDDPFTFGRIAACNSLSDVYAMGGRPLTALSIVGF
                     PIHVLPGTALSEILRGGMSVLQEAGATLLGGHSLDDEEVKAGFAVTGLVKREQLAANS
                     GARPGDALILTKPLGTGIITLATQLGRASDAARAAAAASMTTLNKSAAEIMVRHGAHA
                     CTDVTGFGLLGHLARVVRESGVTAEIRADALPLLPDVLGLAAAGVFSGANERNAEYSA
                     DITDIESGVPAPLRALLFDAQTSGGLLIALPADRAEAALAEMRAAGLAQAALIGRITE
                     ASDGQIRVA"
     gene            complement(8489..13081)
                     /locus_tag="PK634_02245"
     CDS             complement(8489..13081)
                     /locus_tag="PK634_02245"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96588.1"
                     /translation="MRILPVLAAAGGLVCVTVRAGASTRYVAASGSHSSPYTNWATAA
                     HDIQTAVDAASAGETILVTNGIYDTGGRAMDSGMTNRVVINKAVTVISVNGPDFTFLV
                     GQPCPTNGANGSGAIRVAYLSGGAVLAGFTVTNGYTLALEDSGSAKTSGGGVYVSGDG
                     MISNCVLTGNAAYYYGGGAVDAAVHRSILAGNRARSSGGGADESQLYNCILVGNTSYG
                     GGGASYSRLYNCTVIGNTATNRGGGVSYSYCFSSILADNDAPSGANVYFWSYTNCCLY
                     PATTVGGNSITNEPGFVDGPDGDYRLSATSACINAGLVMPWMTDARDFDLNARVVGAS
                     VDIGAFEYQGLSDPLLVEIVAADLQAVVGYSVPLAGRIVGNAEGFVWQFGDGRGATNQ
                     LYVTNTYAAVGLYEVTLTASNLAGAVVATAAVEIVSADYAYYVATNGDDAAAGTNWAT
                     AKATIQAAADAAGPGCTIWVTNGVYDRGGRVVYGSLTNRVALDKPLFLRSVNGPDVTI
                     IAGAPNADGVPDGAAAVRGVYMASNTVLDGFTVTNGHTLAEGDTTRERSGGGVYAGTT
                     NAFITNCVITGSTADRYGGGCCRGTLYNCRLLGNAATNMGGGAYSAVLFGCLVTDNAA
                     LNGGGLASSAARNGVVRGNRAAQYGGGLYSAAGASESCTVAGNSAGTSGGGAYRGSLL
                     NSIVYYNDAPTGPDFFEGDFTNCCVASWPAGANNITNPPGIVSTLDPRLMPGAAVIGR
                     GTNQPWMAAAVDWDGAPRITGASVDMGAFEFVSESECTGALSAAISCAYTQAPAGFSV
                     PFEALVAGRVQGYEWDFGDGARATSGCVVGHAFAAAGVFPVVLTVSNLAGSVAATTEV
                     AIVEPDCNLYVRPGGDDGAAGTNWVTAFATIQAAVDAASLGCTIWLTNGVYTNGGRAV
                     EAGLTNRAAVDRVVTVRSVNGPAFTAIAGQGGIGPGAVRCVWLGSGARLDGFTLTNGF
                     TLATGTSEKQGGGIWAEGSLAVVTNCWVAGCGAAGSGGGGYGGNYQACVFDGNTAARG
                     GGVSAAELEGCTVTNNRASYGGGTEYGTLSDCRISRNSATNGDGGGAYGGTLTGCLLS
                     GNTAANAGGGAYNAQLSECTLLSNLVSGATGEGGGAYGGTLVDCALVQNSVTNHPSFG
                     GGASAVDLVGCTLTSNRADYGGGAYGGSLSNCAAGWNGAAYGGGAYDSLLNDSSLHHN
                     AASNGAGLYLGSAYDTVFSNNTAVAGGGGACASVLHNCRLLHNDAGEGGGAAGGTIYN
                     CVVLNNTADYGGGVASAESYNCTIVGNDATSFGGGTFWGTPRNCIVYYNTAFASVNAY
                     FGWLTNCCSTPMPDGTDNFIAAPRMADYAAGDVRLLSNSPCINAGTNQDWMIGSRDPD
                     GNHRIILNVVDVGAYEYAYPGVDHDGDGIETAYETGTGVYAGPENTGTDPLSADTDAD
                     RLTDGDELTAGSDPTDDASFLGMILPSAQDAAGLIVSWQSAEGKTYRLERSSNLVWGF
                     DYLVQTNIPATPVVNTVTDATATGTGPYYYRAGVEP"
     gene            13222..14475
                     /locus_tag="PK634_02250"
     CDS             13222..14475
                     /locus_tag="PK634_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96589.1"
                     /translation="MHSSRLWTILLTGLGIMTASAQDKLPSNVSFRLKEADRILARAE
                     RALESNSVASEEWKIETAKTAAVEAREKMSEIEQRYAGQYSPSHPDIVAMQGRIAKLE
                     AAAGGRADAMQQAQAAAGQQAAAAGEASEPWLARMRPHVIGLGQSSHDPDKYLVSSAT
                     QDPAEMQKRLGIYAEASAALAEYKKAGLGDLATDDLKQVAALLENALVQFESSCRQYA
                     EQDLRDADAAIGRLEQFIREQDVNMASKETFLFPERDQIGNAQEIVDRAAGLLKTGDQ
                     RINAMNRRIENLKRADARLRAARIAETRMAPDAYAGADAAELKKSAGQFVLRAQPGAR
                     VLKTAIISPDWKQESALEWTDTTQTALRHRTTRSLTAQVAGKRNADTLLYTVAVSQDL
                     QAGGGWGPTYGHIMFTDPILEANVP"
     gene            14477..14896
                     /locus_tag="PK634_02255"
     CDS             14477..14896
                     /locus_tag="PK634_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96590.1"
                     /translation="MKRALSFGLAFLLFGLVCPARAEPDVTWEEILQRLDLARSPEAV
                     FRDVVRPLIDRPATLEATFEKSDTDEAGRPRQQFRHVSGRHTFVCYTDAESAPAAPGW
                     HGAITGLLVELDFKGEDPQGRRYYILRLRQAGTTRLP"
     gene            complement(14939..15898)
                     /locus_tag="PK634_02260"
     CDS             complement(14939..15898)
                     /locus_tag="PK634_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96591.1"
                     /translation="MPVRVNEVRVRRFAEYFAEIGQVSPFDFCSAGEGTLFPVRGCAG
                     VIDYFFFVTAHQFGFWHLDGGRYGGPMIAPVDGVPRKGSDFIVACATRAWNRDPLFFA
                     REELARAGEEGWAGIFRDDAGVNPLPMWPEHREIIQRYAAWLRAAPRPPAELVAAANA
                     APEPLKAFLDEAGKVPGYAEDPMRKKLQLLAIILENRPEHFLRVTDPWNHRPIIDYHL
                     QRSALRTGLVDVDDARVLADLEARRLVSADTEQDIRSAVYHAVARLVEQSGMSVAAID
                     YFFFMNRTRCPEMREPDCPSCPVREFCARRTKLFQPVFRTEAY"
     gene            complement(15906..16565)
                     /locus_tag="PK634_02265"
     CDS             complement(15906..16565)
                     /locus_tag="PK634_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW96592.1"
                     /translation="MADAEKKEVQAYFDRVAGDFDSIYSGRKSGFARWLDRVFRRDMY
                     DRMRLTLEECSAPELKSILDIGTGSGRFCLPLAPGKERVVGLDFSPAMIELARKHARE
                     QGVADKCRFEVGDFLQARFDEPFDAIMAIGLFDYIREPLVFLKRMKDSVRKKVVATWP
                     TVWTWRAPVRWLRLKLQGCPVYFFTPAQIRKHYADAGFAVTRLERVGKIYFVVAAPAV
                     P"
     gene            complement(16580..17704)
                     /locus_tag="PK634_02270"
     CDS             complement(16580..17704)
                     /locus_tag="PK634_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW96593.1"
                     /translation="MLAIFLISGFVLGYTLCLYPVMIGALARWRGRPWMTIGPGAALP
                     PVSMVIPVFNEERILGEKIANCLALDYPADRIEFIFALDGCTDASESVVAAVGDPRIR
                     CRSFPVNRGKVAVLNDAIPAARGALVVISDASGMVNPGGLRELVAPFADPEVGGAAGV
                     YRVSRADRSQLDSAEHSYHGFEMKLRGWEGLFRTTLSGTGSLFAFRKAEFEPFPAGLI
                     NEDYVQPARLALRGRRVLYIPGAQLVDRLSTRPAAVFRRRVRIAYGNWQQIRYLRGLL
                     NPARGYLFWVFVSHKLLRMLLPFVLVLFLASSAVLGPWVAWSVGLGAAGLVLAGAAGL
                     WLDRHFRNFNPLSVVALVFINIVAVFVGTGKYIAGRKAAW"
     gene            complement(17724..18542)
                     /locus_tag="PK634_02275"
     CDS             complement(17724..18542)
                     /locus_tag="PK634_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOW96594.1"
                     /translation="MKHILSVDLEEWFCVANFEKAIPRESWAARESRIEAQTRALLDL
                     FDRRQARATFFILGWVGERHPDLLKDIVARGHELASHGYAHDLVHRLTPEAFREDLAR
                     GLGVVRDITGLSCRGYRAPSFSLRRSDTWAWEALAEAGIGYDSSVFPVMHDRYGEPDA
                     PRAPFDVPAGGRMIREFPLSTVRVAGRNLPVAGGGYLRLYPLGVTRWAIRRIEAEGLS
                     AVVYLHPWEIDPEQPIPPAHWLRVWRHRVGMKSLLHRLDVLLDAFEFVPFRDMA"
     gene            complement(18599..20599)
                     /locus_tag="PK634_02280"
     CDS             complement(18599..20599)
                     /locus_tag="PK634_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HOW96595.1"
                     /translation="MNSRIYTGTVWHARHEPVKHEFEFPGYWVALDLDELPELDRQIP
                     FFGYNRRRLVSIRDADYLDAGPGTIREKLLRRLGAEGCADGIERVILVTSARLLGYVF
                     NPVSFYYALRADGSLRCAVAEVNNTFGDRHLYVLKGEEAAPVTAWPVGFMHPKEFHVS
                     PFNDISGRYRFSFADIRTALDIRVDLETDDGRPLLQAAMKGEGIPLDRASLKRTLRNY
                     PLNVFLVMPRIVWEAAKLYYRKKLKVHHRPEPVHSMTIRRAAPTRLERLYERLAVRFF
                     SQVRKGRLELALPDGKTVTAGDPAASTAVLRIQSREFFRRLILDGDIGFGESFMAGEW
                     TTPDLPGLLRVLADNLASVDDRKIALSWLGRSINRLRHSLRRNTVRVSRRNIRDHYDL
                     SNDFFRLWLDETMMYSCAIFESPAQSLYDAQMNRLRLLIRKARIRPEHHILEIGSGWG
                     SFAIEAARATGCRVTTITLSTQQLELARQRVREAGLADRISVELCDYRHVKGRYDRIV
                     SIEMLEAVGHEYFGAFFRACDRLLKPDGLVVLQVITIPDQRYDMYRRATDWTQKHIFP
                     GGVLPSLTALSRAMTRHSRFVVEHLENIGPHYARTLREWRERFLASKEQVRALGFDDV
                     FLRKWEYYLAYCEAGFAARVINDLHLVLTRPNNPELDRPDSP"
     gene            complement(20596..21864)
                     /locus_tag="PK634_02285"
     CDS             complement(20596..21864)
                     /locus_tag="PK634_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW96596.1"
                     /translation="MSAPLDIAVIGAGGAGLTAAWLLDRAHRVTVFEKNDYLGGHVRT
                     IVLKDGPDAGAPVDTGFIVMNDRNYPTLTRMLDQLGVPLQNSDMAFGFTDENTGQCYS
                     SDLWRGLFARRRNLVSPRFWRLVADILRFYRSGLRDLSRGLDAGLSLGEYLRRGGYSE
                     SFIRDHILPMGAAIWSTPPGQMMDFPAAGFLQFFRNHGLLELSNRPQWKTLVGGSHAY
                     VKKIQAGFRGTFHARAAAASVKREPDGVTVRLENGEARRFDRVVIAAHADEALKLLAD
                     PDGAEQRLLGPWSYSRNDTVLHTDAAAMPPARDAWASWNYIRERGAPPDAPASLTYWM
                     NRLQSLKTRGEYFVSLNRRQPIPAAARLAAFDDTHPMYTAASMDTQRELADLNGRRNT
                     FFCGSYFSYGFHEDAVKSGAAVARQFGIEL"
     gene            complement(21861..22904)
                     /locus_tag="PK634_02290"
     CDS             complement(21861..22904)
                     /locus_tag="PK634_02290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HOW96597.1"
                     /translation="MKRVIDWMERGLVPDALIRAGIRRLHAERLRMEGAGGVEAIRVR
                     HESFIQHMRRSPVAVATDKANEQHYELPAEFFQLILGPRRKYSSGIWPDGVNTLAASE
                     EAMLDLSARRARLADGQEILELGCGWGSLTLWMAGHFPGARITAVSNSHGQREFIQAE
                     CGRRRLDNVRVITADMNEFQADLLFDRVVTVEMFEHIRNWEELLGRVARWMKPDARMF
                     VHIFTHRELAYPFETGDATDWMARYFFTGGIMPSDSLMLYFQRDLRIEEHWQVNGRHY
                     ARTLEAWLEQLDAARPEALAILEKAYGAAEKRRWLQRWRIFILACAELFGYRGGEGWG
                     VSHYLLRKPGAAG"
     gene            complement(22901..23692)
                     /locus_tag="PK634_02295"
     CDS             complement(22901..23692)
                     /locus_tag="PK634_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOW96598.1"
                     /translation="MTSAWVIPAGWAAMVAFFGLLWLVQRRTGDAGIVDVGWAAGIGL
                     VSVFYAAALDGDVARGVLVAALACGWGFRLAAYIHGRNHGKPEDGRYRQLRQEWGANF
                     QRRLFNFYQFQAVTVIILSLSPWAAMRNPASLVGPWTAAGIAIWILSVGGEALADAQL
                     ARFRRRPENKGRTCRSGLWRYSRHPNYFFEWLHWWVYVLLAVGWGGFWLTLIAPALML
                     YVLLKVTGIPATEAHALASRGEEYREYQRTTNAFFPWFPKRSASP"
     gene            complement(23689..24567)
                     /locus_tag="PK634_02300"
     CDS             complement(23689..24567)
                     /locus_tag="PK634_02300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOW96599.1"
                     /translation="MGVHKVTGASSILRRLDWKEGLPLLFMHLACLAVFWTGVSRAAV
                     AACVAMYTVRVFALTGGYHRYFSHNAFKTSRAFQFVLALLGAAAAQRGPLWWASHHRH
                     HHRFSDTEDDIHSPTRRGFFWAHLGWLLVPDYSRTRYDLIGDFAKYPELRFLDRHPFV
                     APAALVLFLYILGEGLAPAGGANGPQFVIWGFFISTVLVYHVTFCINSLMHMVGSRRY
                     ATPDTSRNNAVLALLSMGEGWHNNHHRYAVAARQGFFWWEVDLTYYTLRALAAIGVVW
                     DLREPPAKVYEEARAR"
     gene            complement(24613..25122)
                     /locus_tag="PK634_02305"
     CDS             complement(24613..25122)
                     /locus_tag="PK634_02305"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOW96600.1"
                     /translation="MEESEARENRPDGYFRRKVGGALLPLFRQGVTPEKIAQCLVLGA
                     VISVFPVLGTTTFLCAVVAVRLKLNLPAIQAVNWLFGGVQLALIWPFLRLGEWVFRVE
                     PLPLSPAELAARLKEHPWEFIQSFQAAVLCAVTGWLLAAVPLAWLTYRIMMGVLVVRD
                     RGKKLPVEA"
     gene            25275..25613
                     /locus_tag="PK634_02310"
     CDS             25275..25613
                     /locus_tag="PK634_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOW96601.1"
                     /translation="MSTRSKKVIVAVLILLASVLYGYTMLTRQSPWEDGVFYEVASDV
                     ISLVYYRADQDTLTLAFRSGPVYEYSSVPASVFYGLMDSHRKGSYYNQRIRGQFPSRR
                     LELSAPGEEI"
     gene            complement(25690..26691)
                     /locus_tag="PK634_02315"
     CDS             complement(25690..26691)
                     /locus_tag="PK634_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96602.1"
                     /translation="MTGNAPLLEIRDLRTWFPVRRGLLNRLVGHVKAVDGVSLHVMGG
                     ETLGLVGESGCGKTTLGRSIAGLERPRSGQILFQGTDMKVLSRAERKRAARHLQMIFQ
                     DPAASLNPRLTVLDALTEGLQEHGLLEGSREAEGARLLAEVGLPPEALYRYPFEFSGG
                     QRQRISLARAISLRPALVVCDEPVSSLDVSVQAQVINLLMDLREKHRLSYLFISHDLS
                     VVRHLSDRIAVMYLGRIVEEGPAETVMGSPLHPYTQALLAAVLAPGQPRGRKLVLPGE
                     PPSPLNPPSGCPFHARCPKAMDQCRLDYPPASATETRRVHCHLYPPRVPDPAPLLAP"
     gene            complement(26688..27668)
                     /locus_tag="PK634_02320"
     CDS             complement(26688..27668)
                     /locus_tag="PK634_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96603.1"
                     /translation="MGALLQIQDLTVAFETDEGRLCAADRVSFDIEEGEVLGLVGESG
                     CGKTVTALSILRLIPSPPGRIESGSILFRGRDLLALPASELRRVRGQAISMVFQDPMT
                     ALSPLHRVGDQLVETLQLHQKIGRRAARSLAVEWLKKVGIPAAAERMDAWPHQLSGGM
                     RQRVMLAMALMLEPALIIADEPTTALDVTIQAQIFNLLLDMKDRSTSVLLITHDMGAV
                     WAMCSRVVVMYASRVVEAGPAEALFRAPLHPYTQGLLRSMPSVQAPGGRLEAIPGQVP
                     SPLNYPTGCHFSDRCPKVFGRCRVAEPPLYTRGEGRGAACFLLDEAGIGP"
     gene            complement(27738..30893)
                     /locus_tag="PK634_02325"
     CDS             complement(27738..30893)
                     /locus_tag="PK634_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HOW96604.1"
                     /translation="MDARGRALAKLESIQFHRNDLRSMRADRNGGIFYVCDFGGMPAA
                     EPGRSAEPGAMSLDSTRDILASISVTNPPAYSSKPGSTNVLFLDFNGHVVSNTMWNTA
                     YTQTVWNTTPFDTDGDNTTFSDLEQRYIRQIWERVAEDYAPFDINVTTVQPPLWHRRT
                     GHALITPTTDAGGKRCPHYGYGGIAYVDVFNYASYSYNAAGCYSPAWITIMSGDSYAN
                     TAEAAAHELGHNMGLSHDGTSTQEYYNGHAATTNAASWGPIMGTGYGRNLSQWSKGEY
                     YDANQPEDDLAIISAKLTYKTDDHGSNSASATPVTVDGLSVTATGLVGRTSDRDVYSM
                     TSGAGVVALSVIPYRCTNGTWGSNSDLVLLLCSSNGTVLASNNIAAAAQASITQSVTY
                     GTYYAHVYPSDAGAPTNTTRSGYTAYGSLGAYALTGQVAVLGEEAPVFDPRGTQTATT
                     GVALAFTVSASGYPAPVLALRSATASSGYSFTPATGELNYTPPLADVGARSFTFTASN
                     SAGVATQVVGVTVVEGPPAAPAAIWASETNAAGFTAAWTAVPGATSYRLDAGMCEDFV
                     DLGGGVLLNEDFQAWNGSTWTNGWTQNSAAQYSSGGVTNSRCVGMNGAGDWILAPACT
                     NPGVLSFYIRTSTDPGNWTAVVQTSSNGTDWIDRASIVESGTGGTIDNTPYQTNIPLN
                     LAGTQYVRWYMTARSQDSCYIDEVQVTRMESAGPCYLPDYSNRVVSGTSQAVTGLTPS
                     ATYYFRARAVSPGGTGVYSSVANVTLPEPAATNQLIVSSLCGNPDPVVGVNWFTSGTW
                     VSAAVGGSPWVSGQTQYVCAGWTGTGSVPSSGSLTNTSFSLTNNSTLTWRWTTNFYFA
                     RTAGTNGAVLGPASDWYPFGASVTVTAAPAEHYCFAGWGGDTQGDTNLTVMTVSLDRP
                     RQVLASFVEIMAACGTPEPWLAAFYPATNDYDAAELTDTDEDGLSAWKEYVAGTDPTD
                     PDSGLELAPAAPVGNQVVVAWPAISGRYYSVYYLTNLAGSVEQPVIENWRAGQTGPAS
                     STNAIAPDVLKRYYRLKVSLE"
     gene            complement(31178..32794)
                     /locus_tag="PK634_02330"
     CDS             complement(31178..32794)
                     /locus_tag="PK634_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOW96605.1"
                     /translation="MTTDVQERRRRWGLNPVTRKRLQRFRDMRRAWGSFWILIVLYAI
                     SLVSELVAGNQPLYMRYRGRSYFPLLRFYPEDVFTGSGRMTRPDYKQIAGSEAFRSDP
                     GRRMVFPLIPFGPLETVRPDSIALPDQVLLTLTREAVVGAVSLSSDGAILSAENCAFF
                     LGVPDADMAGRNIDFFMELPAAVRDGLRARFQNRAAASVSAEAPLRKAGTGLLSLSTY
                     TPRREAPGSVRMLLREAGAGRAATEKLRVDRDGRVATGRPPLWSDLDASRQRILLDKA
                     RSRFEGPVSEERWQEGERWLRAAFDKESVTYPWRPCPGHPLGLDSAGRDVLVRILYSL
                     RTSLTFGLLLVVVTMLVGVVVGAVQGYYGGWVDLLGQRTIEIWESLPFLYILILMGSV
                     FGRSFALLLICYGIFNWIGISYYMRAEFLRLRKQAFVEAAWCMGLPAWKIIFRHILPN
                     ALVPVVTFFPFSLVGAVGVLAALDYLGFGMPPPTPSWGELLAQAQEFNWAWWLSLYPS
                     LALFIVMLLGVFIGEGVRAAFDPRRYSRLE"
     gene            complement(32791..33834)
                     /locus_tag="PK634_02335"
     CDS             complement(32791..33834)
                     /locus_tag="PK634_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOW96606.1"
                     /translation="MDRLDYILRRLLLMIPTFIGITFFSFGLCQFVPGGPVEQVILQM
                     RGLGAGGERMSSAAGPITEEQRKALEKHFGFDKPLPQRYWKWLVHDRIGLAVESYKYP
                     NQTVWQLIVKRFPVSLVFGITGFVLTYLVCIPLGIAKALRHGGAFDLASSLIVFIGYA
                     IPPFAFGMVLKMLFCGVTETFWDLFPVSGFHSAGFAAMSLGAKAKDLFQHMFLPVLCY
                     MVGNFAVLTLLMKNSLLEQIGQDYIRTVLARGATRARAIWGHAFRNALIPIATGFGGI
                     LTVMFAGSVLIERVFEIPGMGRLSLDAIVSRDYMVFMGILSATAILGLLGRLLSDFCY
                     LLIDPRISFRNQS"
     gene            complement(33847..35721)
                     /locus_tag="PK634_02340"
     CDS             complement(33847..35721)
                     /locus_tag="PK634_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOW96607.1"
                     /translation="MKIILITSAFLLLASMAVPGSDALFPGDGWKDAPNPLASPEAVP
                     GGEIAIYAGDYPKSFNYYLDQNVFSAELFGAMYETLLGQHPLTLDYEPGLAERWAISE
                     DKRTFTFWIDPRAAWSDGRPVTAEDVKWTFDAVMKPENITGPHKIFLGRFESPEALDE
                     RTIRFHAKEIHWQNLDALASFQILPRHAFADADFNKINFEFPVVSGSYRLGEIQEGVL
                     AKLERREDWWTRGSARARGVGNFQTLVFKFFAERDNAFEAFKKGEIDLFPVYTAHLWA
                     TQTEGERYEKNWVIKQRVFNYNPIGFQGFAMNGRRPPFDDIRVRRALAHLLDRETMNA
                     TLMHNQYFLHRSYFEDLYGADHPCENKPIRFDRERARELLAEAGWTVNPKSGLREKDG
                     KPLRFRFLSRDAATDKFLVIYQEALKDAGVEMEIEKKDWAAWAKDMDEFNYDMTWAAW
                     SGGLRKDPEPMWSSTEADKRSGQNIAGFRNAEVDALIASQRGNFDIAQRHDICRRIDR
                     LLVAEAPYILLWNINYTRLLYWNRFGAPPTVLGKYGDERAAYWYWWYDEDAAAELKDA
                     MQDGLPLPRKPADVRFDEVFREAPGTGIRTEDAKDAKAPQGEGDDKQEVAPEPLNPNP
                     "
     gene            complement(35739..37433)
                     /locus_tag="PK634_02345"
     CDS             complement(35739..37433)
                     /locus_tag="PK634_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOW96608.1"
                     /translation="MLLLLFLPACGDRIASPAARAWRSQTARIRTLDPARAADVASAQ
                     AVSRIYEGLLQYAYRDRPYRVEPLLAEALPEVSPDGLVYTFRLRRGIRFQDDPCFPGG
                     KGRELTAEDFVYSIKRLADRKTAASGYWIFRDRLAGLDDFREASAGSEPTDYSQRVEG
                     ALALDPHTLQLRLTRPYPQLLWVLTMPYASAVSREAVEYYGAEFDRHPVGTGPFVLAD
                     YRPNYRIEFARNPVWAAAGRIDRDAEGHPIPALDRVVQYVIGDPATAWLMFLDGRLET
                     SAVSRDNWDAVVRGGGDLEPALAARGIRMVRAPTLDLFYIGFNMDDPVVGTNRLLRQA
                     LTCAFDTGEWTRFYNGRIVRPNGPIPPGVAGAPEGEGPFPFDLERARRLLAEAGYPDG
                     VDPRTGRRLVLTLDLGQADNPEMRQSTDLFIQFMDRIGVVIEPRYNNWPTFLGRIERR
                     QVQMFRLSWIADYPDAENFLQLFYSPNASPGSNRSNYRNPEYDRLCEQARVMPDSDER
                     TALYRRMASIIQEDCPWIFTHHPAACVLLQPWARNFEYHDFPYGMEKYYAVEDPEP"
     gene            complement(37522..37977)
                     /gene="secG"
                     /locus_tag="PK634_02350"
     CDS             complement(37522..37977)
                     /gene="secG"
                     /locus_tag="PK634_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOW96609.1"
                     /translation="MLMRILLVVVEIICSLALIGLILIQRSKSEGLGLAFGAGMGESL
                     FGSRAGNILQKLTVIFAAVFLVTTTLLALTYSGDRAQSLMSSQMMGRPAAPVAPSGPI
                     AGPGPDAPAGMGLQEAPAAQPASPMEPAAALPAEAPAPVAEPQPAPAAE"
     gene            complement(38004..38759)
                     /gene="tpiA"
                     /locus_tag="PK634_02355"
     CDS             complement(38004..38759)
                     /gene="tpiA"
                     /locus_tag="PK634_02355"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOW96610.1"
                     /translation="MRKPIVAGNWKMNKTVAEAVALVAEIKAGLQGLDAVDVVLCPPS
                     TALQAVGQAIAGSKLDLGAQNMHWEKSGAFTGEVAAPMIRELGCHYVILGHSERRQYF
                     KETDEIVNKKAKAALAAGLRPIVCVGELLEERESGRTEAVVGAQVRGSLAGLSGQDLL
                     KTVVAYEPVWAIGTGKTATPAQAQDVHAFIRRVLKERSDDHIAQSVRIQYGGSVKASN
                     AAELFHQPDIDGGLIGGASLEAASFLAICRGAL"
     gene            complement(38848..40104)
                     /locus_tag="PK634_02360"
     CDS             complement(38848..40104)
                     /locus_tag="PK634_02360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOW96611.1"
                     /translation="MNKLTIRDVNFKGRRVLCRVDFNVPIRDGVVNDNTRIAAALPTI
                     KRVLDQGGALVLMSHLGRPKGKGFEAEFSLKPVAARLQELLGRPVQFGGDCIGAEALA
                     LAQALKPGEALLVDNTRFYKEEEGKVKLPDDTPEEQKKAAKAEMKAKQKEFAKKLAAL
                     GDGEIYINDAFGSAHRAHASTAIVCQFYKKNVAGLLMEKEIDFMGRALASPERPFVAI
                     LGGAKVSDKVNVITNLLTKVDALVIGGAMAYTFYRAKGLATGKSLVEEDKVDLAKDIL
                     AKAAAAKVKLLLPVDNVIADKFDAAAQTRAVGESIPDGWMALDIGPKSAALFAAEIRK
                     AKTVVWNGPMGCFEMAPFAAGTLAIAKAVAETKCLSIIGGGDSVSAVNKSGLAGKMSH
                     ISTGGGASLEFLEGKQLPGVVALTDK"
     gene            complement(40209..41231)
                     /gene="gap"
                     /locus_tag="PK634_02365"
     CDS             complement(40209..41231)
                     /gene="gap"
                     /locus_tag="PK634_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOW96612.1"
                     /translation="MAIKLGINGFGRIGRLVFRAAVENPKVEVVGINDLFDAKQLAYM
                     LKYDTIHGPFKGTVKAGEGALIVNGKSIRLTAEKDPANLKWNEVGAEYICESTGLFLE
                     KAKAEGHIKAGAKFVVMSAPSKDDTPMFVMGVNHKNFKGEQFVSCASCTTNCLAPVAK
                     VLNDNWGIMEGLMTTVHATTATQKTVDGPGGKKDFRGGRAAAGNIIPSSTGAAKAVGK
                     VIPALKGKLTGMAFRIPTLNVSVVDLTCRLQKAPGADAETAYAAIKAAMKAASEGELK
                     GILGYTEDEVVSSDFNHDARTSIFDAGAGIMLNSNFVKVVSWYDNEWGYSNKLVDFIV
                     YMASAK"
     gene            41422..42246
                     /gene="thiD"
                     /locus_tag="PK634_02370"
     CDS             41422..42246
                     /gene="thiD"
                     /locus_tag="PK634_02370"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOW96613.1"
                     /translation="MNDIPPTPDAPVALTIAGSDSGGGAGIQADLLTFAAFGVFGTSA
                     LTCITAQNPREVTGIQALPPEFVARQIKTVCDAFPVAAAKTGMLFSSPIIRAVAAADV
                     QQGIPILVVDPVMVAASGARLLEADAIEALCGELLSQARVITPNLDEAAILCGRAIES
                     VEQMREAAREIGDRYDVACVIKGGHLAGPEITDVLYDEGQELVMAGARLAQAETHGAG
                     CAFSAAITAGLAKGWLLEDAVTRAREFVRQAMESARPAGAHRPLHFLANYEPDASD"
     gene            42253..44781
                     /locus_tag="PK634_02375"
     CDS             42253..44781
                     /locus_tag="PK634_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOW96614.1"
                     /translation="MAVEPMELAAGTDGRASPRRPFMGLAAFFLAGVGAGLCLDVPCP
                     LLGAGAALLAVAGMLARHAAGSLLTGLAVLLAGWLNVSLAARSPSPREVHALMARDRE
                     QVELVGLVTGDSVTTAARREGQVICRFSLRVEGLHRKTDWERARGTVSIFWRKDAAGP
                     EPRYGERWVWRGVIERRPRFESGGGGSLCGMTPDPDGGRKLSSGHGWKFVSWCLAQRR
                     ACFDLLGLGLERSPDVAALLRTLLLGYRQELSPQLLQAFSTTGTLHIISISGLHVGIM
                     VVLITALLKALGRPRPRWALYLAPALLVYSISTGLSASTVRATIMAMIFWAGPLFRRR
                     PDGLSALALSAILILAFAPLQLVDLGFVFSYSAVAALMMWYPVWVTPFAARLRADPWA
                     PPESKGRRILRQLALYVFSMLLTTAAVWITTTPLSARFFNLVAPMALPSNLLVIPLSF
                     VVLVTGVLTVLFGALSSWMAEVFNHANRVFTSVMLESIEWFSSWRGGYTYVPTPPAWF
                     VAAWFAWMALLRMARRRAVIAAAGLVMLGAGWAWILRGDEACADVLDVGQGQAVFLNL
                     PGSRDVLVDTGPRFSSRDVIRHLRRHGVNGLRALVLTHGDAEHIGGTAAVLDALPVDE
                     IWCAPHLGRSPAYRELLADIRRRGIPLRALTRGDRGLLPGGLEWEVLHPPADRNFRRS
                     DEATLVLRVGREATALMIMGGADGSVEQDLVAQPADLGATVLVTGDHGAEGTCRIDWL
                     ERVGPVAAVISVGLGNVRGQPDRATLSRLREKSIPTWRTDESGPLRIPFQHSAPGRKT
                     PAVQPLVSPAPPVPGAVPGSPDRSERDQTPDGTSMPAPASGPPG"
     gene            complement(44666..45751)
                     /locus_tag="PK634_02380"
     CDS             complement(44666..45751)
                     /locus_tag="PK634_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96615.1"
                     /translation="MDPLSHSVYHVRDKAPAGAPPAAGPPQAPEGYWAESQRQLRRQT
                     LLFVLTVLVSGLLIVVVTTYYYLQISESRQVARTPRRTLERPASPLRFEADAQTMYLL
                     EELKPPAAPEELKDLPLTSASLKQAAYYLVKAERAAKQERFDDALDSYQKVAQIFPDI
                     RGVQGQVGLLAIRLKQFAVAAEAFEKASREEPMTFSMANNLGVAYMGMEDYDKAEASF
                     LTATRMNPQYANSYFNLATLYLRRGQPDKASQRFEEYMTLQPGDTMAGQTYALILLQL
                     EQWDRAAKLLQQIARVAPDVAPIHFRLAQALSHGNSPESAIDALKRAISLVDPKQALA
                     WMSRPEFDLVRNDPGFQELLLELGAQD"
     gene            complement(45755..46963)
                     /locus_tag="PK634_02385"
     CDS             complement(45755..46963)
                     /locus_tag="PK634_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96616.1"
                     /translation="MNPISSPPPDAEPGGTGGAAGYQYHRQRQNDPSRRGRDSRPAPR
                     RDNRATSHAAWYTVLLVLASMGMFLLIVLFISRKTWQMKENARTRDIPDSASVTGAPV
                     DLDRKRVETQELLSGVRRSDAELANEATLLARRGDLLQSAGQYVQAAEMYSEALRLWP
                     DLNSARGELGRLNLRQREYNKALIVLQSAIGTEPDSPALLNDLAVAYFYQNRAAKAAE
                     LLEAALQYDPQFAPAHFNRALCFLLQSDAAAAAAELERYLAQAPGDPKALKEQAFLEA
                     SRSRYAEAYAIQAGALQKKPDWIPLIMDAAAVAALMGDMDKARAHLEHAAAKAPPSVV
                     LQSFRGPAFAELRKTDAGRAFESELEKKDRARQLTGGDEAVAPWDAYSNEPLLSSSLS
                     EKAPETAETE"
     gene            47120..48415
                     /locus_tag="PK634_02390"
     CDS             47120..48415
                     /locus_tag="PK634_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOW96617.1"
                     /translation="MSLRTLGRGVGWLALAVLLTANLVLGARLQNQAGPPDERDTGYE
                     KMALFTRVLEQVREQYVDADKTTYQDLIYGALRGMLESLDPHSQFLDPDMYKDMKDET
                     AGEFGGLGIVIGVKEGMLTVIAPMEDTPGFKAGLLPGDKILEIDGKPTDNLTMEEAVK
                     QLRGAPGTKVTLGILRPKSEGIRTMELVRAVINIPSVKDTDMLEDGIGYLRIVQFNEP
                     TAKAVHEAIQGLQARGMRALVLDLRNNPGGLLTSAIEVAEKFVRRGDMIVFTQGRNEK
                     PLQTFRAKGRQHYTDFPMVILINGGSASAAEIVAGALQDHRRAILVGGKSFGKGSVQS
                     VLQLDDGSAIRLTTARYYTPSKRVIHEQGIEPDVPVAMPPEDLQRLINQRAEPESGKD
                     EADVPASAIRDVQLERALDILKGVMIFTAQQRTSAVARR"
     gene            48459..49511
                     /gene="tsaD"
                     /locus_tag="PK634_02395"
     CDS             48459..49511
                     /gene="tsaD"
                     /locus_tag="PK634_02395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOW96618.1"
                     /translation="MNVLGIETSCDETAAAVVADGWKVLSNCIYSQVASHRPYGGVVP
                     EIASRQHVEILPGILREAIGQSGLGWDDLDAFAVTRGPGLASSLLVGLSAAQSLALRQ
                     RRPLIPVNHVEAHLYSLFLPEPPAAAPPPAPPLPMLVLMVSGGHTALVRFEGLGRYRW
                     LGQSLDDAAGEALDKGANLMGLGYPGGPAIEQAAAGGDPEAISFSRTSIRASPRTGDL
                     DPDLCFSFSGLKTALLYLLRRQPELLGPDRLRHLAASFQEAVFNTLLHRAEQAMEKDA
                     YASFGCVGGVLRNRRLRARIEEAARRRRLPLRMAPPSFCTDNAAMVAGLAGATGGPAP
                     ADPAAVEIRPDLRLSA"
     gene            49600..>51734
                     /locus_tag="PK634_02400"
     CDS             49600..>51734
                     /locus_tag="PK634_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW96619.1"
                     /translation="MTASSSPAAAFFKSLVLSLDLPVIVMDAELRVLLLNPAAEGITG
                     ARTRQAVGQFGGELVGMDRATCEALGFGSGGGATGWKEFVCVPAGSASRLVRARGCTL
                     PWSARRTAVVWILDAPGWHDAGPRLLPSGAPVIIFELDAGGRLVYGAEEFAGLLDRQA
                     DVTTGRLFRELLVEEQRGSFDAVWRKAHGGEAVRNSELRLVHGRGAARPFWVSLFPIK
                     DPRGITLGVRGVAGDLSGQKDLAYALEASEERFSVLFRESSDPILILNMEGDILLANP
                     SFERITGVRSDELFSGEKSWSDFVLPADYEALRGELRQCAATHKDGMTEFRARDAGGQ
                     VVWFEQSHSILHDEVGQPRGIMAVARNIHRHKQHEFELREQAEDMQRRHDRAQALISR
                     LKLFFAQTSSLADDPQRFLQGVCDILFEMYEPLLVILHLSGEGRVVHRGRPDVLADVG
                     PEALEQLQCGVCGQITETGMPFYSHSLDTAPPPQCAARLGSRSLKTCVGAHLRDSSGR
                     IRGSIIMLDDKTRAFDSLDVEVVTVAALQVAARLRAEEQETVRRGLEEHLRQAQKMEA
                     VGMLAGGIAHDFNNILSSILGFTTYLLARTPPGSNIHRDLGLIEQSAVRAADLTRQLL
                     SFARRRHFAKEPVSFNTVIHDVVAILRRSLPANIQIHMRLAPELPPVLGDRGQLNQVI
                     MNMGINAAEAMSGKVGMLTMPYYFFAEML"
BASE COUNT         8510 a        17900 c        17091 g         8233 t
ORIGIN      
        1 gagcgcggcg gcggggcgta cacctacacc gactccggag cgccggtcgt cgagagctgc
       61 acgttcgcgc ggaacgcggg cgcggaagcg ggcgggatct acctcaacgg cagcgcggtc
      121 ctgctgaatt ccatcgcgtg ggctaacagc gcctcgtcgg attcgaacat tgtcctcttc
      181 ggctcgggcc aggacgtgca gtacagcacc acgggccccg ccgtggcggg aaccggcaac
      241 tcggcggcga atcccgcctt tgtggacgcc gtctcggaca acttccggct gggcgatgcc
      301 tcgccttgca tcgacgcggg gaccaaccaa gcctggatgg cgggcgcgct ggagttggat
      361 gcccgtcccc gcgtcgcgca caaccgcgtg gaccagggcg cctacgaggc gatcctgacc
      421 gcgtgggaca cggacggcaa cggtctgccc gactggtggg agtggcagta cgagaaggtc
      481 ctgaccggca cggatcccga cggcgacccc gacggcgatc acatgccgaa ccgcgacgag
      541 tatccggccg gctcgcatcc gggcgacggg gactcgttcc tcggcctggc ggcgccctgg
      601 gccggccccg cggcagactc gatcgtgctg tcctggcgcg gcgtgaccgg aatatcctac
      661 cgcgtcgaac agagcaccaa cctggcgcag ggcttcacgg cgacgctttt caccaacatc
      721 cccggtgttt ctccctacaa ctcccgcacc gattcggtcg cgggcacggc gacccggttc
      781 taccgcgtcc gcctggagcc gtgagtcacg gcgcgacccg gacccgatag aacccgctct
      841 gcgcgcttcc gattttggcg gtatggatat tggacggcgg cgtggaggtg atgttggccg
      901 ccaactcgct gaatccgctg atcaggtttg aggtctggta cagcgtgtag gtccggttgg
      961 attcgctgct ccaccgcagg acgaaccggt ccgcggcagc ctcctccgct ttggcccgga
     1021 gcagggacgc ggcgtcgtcc aggcgcgtgc cggctacgaa ttccgagagg ttcttcatgc
     1081 catcgccgtc ctcgtcgccg tgctcgagcg cgatcttgac cggctccatc gtcacgaact
     1141 cacagccata gttcccgtac tcatccaccg tcccgtacgc tcgccggccg atatgcgtcg
     1201 ccgagaggta gcggtcgttc ccgttggtgg aatcggaggc gatcacgccc gagaccagga
     1261 gccaggtcaa cgaggtcgcc ccgccggaca cgttgagcgc cgcccagggg agcgaggcct
     1321 cccaccggtc cgtgcgtcgg tccccgtcgt tgtcctccgt gacgcaggcc gtcgtacccg
     1381 tcccgtcgaa ctgcgacagc cgggcatcag ggacggggac gaagaaagac gtgttggtgc
     1441 cgatataata gatgccctgc ccgaaatcgt agccgccgta gatgaaagtg gcgtaggccg
     1501 gcccgtcccc gtactcgtcc ccgaagacga tggccaggtc catcggctcg acgaatccga
     1561 ggttatgaag ctggtccagc gactgcggcg cgccgtcctt gtgccacagg ttctccgcgt
     1621 tgtccgtcag cgtgtccaag cccagaaaga ggacgaacac gttattcgac ccgccgaggt
     1681 cggcgccgat cccgccgacg tacaggttcg agccatcgtg gttgaagtag agccgcccga
     1741 gatccccgaa gcccgagccg tcccggacct ccagcggccc gccgttcgtg ctgaaatcga
     1801 aagcgtcccc ggtgttgttc tgcgcggcgg ggtcgccggt gatcgcgggc gtcccggccg
     1861 cgaccagccg cggcggaggg gaggtcggcg gctcgtacgg catgtcgtcc agcagcgcct
     1921 gccagttata gccgtagttg ttgtcccagt tcgtgaccgc cgcatcgtgg aaggcgaacg
     1981 cgatgcggtt cgaggtcgag gcgggcacgg tgaacgtgta ttcccagaaa ccgctggagg
     2041 cgttccaggt catcgaggtg tccacaaggg cgctgccgaa ctcgtccacg ccaaggtgga
     2101 tccagacggg cgaggcgtag aacagcggcc cctgggcggc gtcgtacagg gtcttcgccg
     2161 tcccgccccg cgcggggaag gccggctcga cgcgcacctt gcgccgcgag ttcgtccacg
     2221 tcaggtcctc gaacacgagg ccgtcgctgc ggacggcccg gtagcgcagg acggatccgg
     2281 tgatctggag ggagacgaaa tggtagcagg tggtcgcata agcatgggcg ccaggatcga
     2341 ggacggtatc gtacaacgag gcgccgcccc cggcggccac cacgtaggta atgtcccgga
     2401 tgggaaccgt gcgctggtag ttgtggttat gcccgttgaa aacgaggtct cctccgtaaa
     2461 gagtaaacag cgggcaccag ttggtccgga tgaactcgtc cccttcgtgc tcgccccacg
     2521 aatagggagg acggtggaaa tacggaatga tccagagcac gttggtgtcg tacgccgcgg
     2581 cctgcaggtc gcgcatcagc cagttcgtct gcgcctcgat cccgacgtcc gaatccagcg
     2641 cctggaagtg cacggggccc gcgcggaagg aatacggcga gcccggtatg tcgcgttggg
     2701 gcaggctgaa gaacggccag aagagagagc tgccactctc cgggtagtcg tggttgcccg
     2761 gcgtcggcat gaagacggtc cggtcgagca catcggtggc gacgttgagg aacagggccc
     2821 agggcgcgta ccccgcgccc ccgtactcct cgtccgagag gtcgcccatc tggatcacca
     2881 ggtccggggc ccatgccgcg acccgcgacg cgacaccccg gcacccgccc ggttcgccgc
     2941 cgccctgcag gtcgccgtag accacgacat gcaacgtgtc ggtggcgacc ggcgccgtcc
     3001 ggaaggtccg gtcgcccgcg gcgaagccgt ccgtggagga aaccgagtaa tggtacaccg
     3061 tgccgggctc caggccgcgc agcgtgatcc ggtggcggcg cacgccgccc gcgtcgcgcg
     3121 cttcgtccgt gtagttggag gtcaagccgt accgaaccgt gccgacgccg ggctcgtccc
     3181 gctgccagaa aacagtaacc gtcgtctgcg gatcctccga ccaggtcagg atcaccggga
     3241 cctcggccgc gcaagccgcc ccggcgaacg cgcccaggcc caggatccag cgccggcagg
     3301 caaaggggat ggaaaccatg gcgttactat acccacgaac ggaaattatt tcatccagcc
     3361 cggttttttg ccgatcacct ggtcgtcccc gccaacctcc atgccggcct tgtccttgtc
     3421 caacttcagt ttgagcaggg ccgccattct ctcgttctgc tgcagcagct tgacgatctg
     3481 ggccttgagg tagatcaccc acgtcagcag caggaaggcc gccaggcaaa gcaagacgtc
     3541 tttcatccca tcctcccggt cccgcgcggc atcctgccat gccccgccgc gaaaacgaag
     3601 ccggaattag gttgccctcc cggcggcgct tttggcagct tggcgccatg aacaaggatg
     3661 ccggcgacgc ttcgcccctt cgcgccctgc cctccgtgga cgccgtgctg aacacggccc
     3721 gtgcgcgcgc cttggcggag cagcacggcc gcgcccgggt ctccggcgcc gtgcgcgccg
     3781 ccgtggagtc gcggcggcag gcgatccggg gcggcgaggc cgctcgcccc gatgcggaaa
     3841 gcctggcgga ggccgcggcc gaattgctgg cggcggaccg gccgaatctc cggcgcgtcg
     3901 tcaacgccac cggcatcgtc ctgcacaccg ggctgggccg cgccgtgctg cccggcgaca
     3961 cgctcgccgc cctcgccgag gccgtcggca actgcaacgt gcagatggac ctggaaaccg
     4021 gcgagcgcac gcggcgcgag gccgtggtgc tcgacctttt ccggcgcgtc accggcgcgg
     4081 aggacgccgc cgtggtcaac aataacgccg ccgcgaccct cctggtcctg gccgcgctct
     4141 cgcgcggccg cgaggccgtc gtctcgcgcg gggagctgat tgagatcggc ggctcgttcc
     4201 gcctccccga catcatggcc gagagcggcg cgctcctgcg cgaggtcggc acgacgaaca
     4261 agacccatct ccgcgactac gaaaaggcga tcggcccggg caccggcctg ctgctgaaag
     4321 tgcatcccag caactaccgc gtggagggct tcgtcgccga gacggccctg cgcgagatcg
     4381 cggccctcgg gcggacgcga agcgtgcctg tgatcttcga ccttggcagc ggcaacctcg
     4441 tcgacctccg gaagtacggc ctcgagggcg agccgacggt gcaggacggc ttccgcgacg
     4501 gcgcggacct cgtgctgttc agcacggaca agctcatcgg cggcccgcag ggcggcgccg
     4561 tcgtcgggcg gaccgacctc gtcaccgccg tgcgcaagca cccgctctac cgggcgttcc
     4621 gcgtggacaa gctccgcctc gccgccttgg aacacgccct gcgcctgttc ctggagccgg
     4681 acaagctcga ggcgcggcac cccacctatt tcatgctcgc gcgcaagccg gacgaactcc
     4741 ggcggaccgc ggaagaattg cgcgccggca tcgcgaagcg gcagccggct tggcaggcga
     4801 ccgtggtcga agaggaatcc aagctcggcg gcggcgcgct gccgacctcc cccctgccca
     4861 ccttcgccgt gtcgctccgg gccgaagggt tgtccgccga tcgattggcc ctcgccttgc
     4921 gccgcgccga cccgccggtg atcggccgca tccgggacga cctcgtgctg ctggacgtcc
     4981 gcaccatcct gccgggggaa accgaggacg tcctgcgcgc catcgagcgt ctcgccccgt
     5041 gagccacccc cctgtcatcc acgtcaccat cggcaccgcc gggcatatcg accacggcaa
     5101 gaccgccctc gtcaagctcc tgaccggctg cgacacggac cgcctgcccg aggaaaaggc
     5161 gcgcggcatg accatcgacc tcggcttcgc gacctgcgcc atgccggacg ggcggcgcgt
     5221 cgggatcgtg gacgtcccgg gccacgagcg gttcatccat aacatggtcg cgggggcgac
     5281 gggtattgat gtcgtgcttc tcgtggtggc ggcggatgac ggcgttatgc cgcagaccgt
     5341 cgagcacttt cacatcgtac ggctcctcgg gatccggcgc ggcctcgtgg cgatcaccaa
     5401 gtccgacctc gtggagcccg cgcgcgtggc cgaggtcgag gcacaggtcc gcgccctggc
     5461 cgcgggcagc ttcctggaaa acgcgccgat gcgccccgta tcttcgaaga ccggcgccgg
     5521 gttcgacgcc ttctacgacg ccttcgcgga ggtcgtcgcc gccacggtcg aacaccgggc
     5581 ggacgggccc ttccgtatga acatcgagcg ctccttcgtg ctgaagggcc tcggcgtgat
     5641 cgtctccggg attccccggt ccggcggcgt gcgcatcggc gatgagctgg aactcctgcc
     5701 cgccggccgc cggaagaaag tccgcgccct gcaggtgtac ggcgcggacg ccgaggaggc
     5761 cttcgcgggc gagtgcgtcg cgctgcgtct ttccgacctg gaccgcgagg aggcgggccg
     5821 gggccgcgtc ctggcgacac ccggctactt ccagccgacc cgcttcttca acgcgaagtt
     5881 cctccacctg cccgacgcgg gatccgccct gaagccgcgc acggccatcc gattccacgt
     5941 gggcacctcc gatgcgcccg gatacctcgt cctccccacg ctcgacccgt tgccgccggg
     6001 cggggagtgc tttgtccagg tccagctcgc cgagccggtg atcgccgcac cgggcgatcc
     6061 gtacgtcgcg cgcgccttga gcccggcacg gacgctgggc ggcgggcacc tcgtcgggac
     6121 agacgagcag aagatgcgcc gatcgcgcgg cgattgggtc ggcacccgcg aggaggagga
     6181 acaggcgctc catgatcctt ccgccgccct gctccacgcc ctccgcgccg ccgggccggc
     6241 ggccctgaaa gcggaggagg ccgcgcatcg tgcgctcgtc gccggggacg cggcgcggac
     6301 ggaactggac cggctggtcc aggccggcga cgcggtccgc ctcggcgacc gctacgcctc
     6361 cgccgactcg ctccggcaac tggccgagga cctggccgcc gcgctgaaac ggctgcacga
     6421 ggcgcagccg aaagagccgg gcttcacccg ggcggacctg cggcgaggcc tgaccgcgga
     6481 gccggaactc gtcgagcagg cgctcaagtc gctgtgcgag gccggccgca tccggatccg
     6541 cggcgagctg gtcggcgagg ccggtcggtc tgtcgtggac gaatcggggg cggccctcct
     6601 ggcggtgtac cgggagaccg ggttccagtc gccgcggccc gacgaactgc cagcgcgtct
     6661 cggctggccc ctcgcccgcg cggaggccgc gctcaagccg ctgctccagg cgggggagct
     6721 ggtccggctg tcggagaagg tcatcctgca cccggagcac ctggaagcgt cgcgtgtgaa
     6781 gctgatcgac tacctgtcgc ggaacgaccg cgtggacgcg gtgaagttca aggaagtgct
     6841 cggcacgtcg aagaagtacg ccatcgcgat cctggaatgg tgggaccgca agggcctgac
     6901 gcgccggatc ggcgacgagc gcatcctggc ccggaagccc ggttgaacca cggaggacac
     6961 agagggtcac ggaggagaat caacgctgct catcattccc ctgtgccctc cgcgacctct
     7021 gtggtttaat tccgtccgtc ttcgttggcg cacaatgact cgaagcgata tcaatgcgga
     7081 gaagcggcgg caggtgatgg gccgctcgat caagctgggg cactgcgtct gcaacccgaa
     7141 gctgccctgc ccctgcccga cgttcaagga gaaggacacc tgcctgtgcg cgggcgagca
     7201 tgtgccggcc gacccggcgg cgccggcggc gcgcctgacc gagcacgtcc ggagcgccgg
     7261 ctgctcgggc aagatcagcc agaaggatct acacgccgtc ctctcccgcc tgccgaaaat
     7321 cgatgatccg cgcgtgctgg tcgggctttc cacgggcgac gacgcggggg tctatgaact
     7381 gacgccggac ctgctgctcg tgcagaccgt ggacgtcttc acgcccgtcg tggacgatcc
     7441 cttcacgttc ggccggatcg cggcctgtaa ctccttgagc gacgtgtatg ccatgggcgg
     7501 acggccgctg acggcgctgt cgattgtcgg atttcccatc cacgtcctgc ccggcacggc
     7561 gctctcggaa attctgcgcg gcggcatgag cgttttgcag gaggccggcg cgacactgct
     7621 gggggggcat agcctggacg acgaggaggt caaggccggg ttcgccgtga cgggactggt
     7681 gaaacgggag caactggccg ccaacagcgg ggcgcggccg ggtgacgcgc tgatcctgac
     7741 caagccgctg ggcacgggga tcatcaccct cgccacgcag ctcggccggg cctcagacgc
     7801 cgcgcgcgcc gccgcggcag ccagcatgac cacgctgaac aagtccgccg ccgagatcat
     7861 ggtccgccac ggcgcccacg cctgcacgga cgtcaccggg ttcggcctgc tcggccacct
     7921 cgcgcgcgtg gtgcgggaga gcggcgtgac cgcggagatc cgggccgatg ccctgcccct
     7981 gctcccggac gtcctcggcc tcgccgccgc cggcgttttc tccggcgcca acgagcgcaa
     8041 cgcggaatac agtgcggaca tcaccgacat cgagtccggc gtgccggccc cgcttcgagc
     8101 cctgctgttc gacgcccaga cctccggcgg cctgctgatc gccctgcccg cggaccgcgc
     8161 ggaagcggcg ctcgccgaaa tgcgcgccgc cgggctggcg caggcggcgc tcatcggccg
     8221 gatcacggaa gcgtcggatg gccaaatacg ggttgcctag cccgcttatt gcatgagcgt
     8281 agagacgctc acgcaataag cgcttctggg cacgcgaatt gcataggcaa ttcgcgtgcc
     8341 agaagacgtg acgtattgtc gaatcaagga ttcgacaata cgaacgtggc aagccccgtt
     8401 tgtgcccgca acgcgggcac atcacggccc tttgggcgga taatgcgcgc caatcgcgca
     8461 ttatccgggc tagagtccga aacggaatct atggctcgac gccggcgcgg tagtagtagg
     8521 ggcccgtccc cgtcgcggtg gcgtcggtaa ccgtgttgac gacaggcgtc gccgggatgt
     8581 tggtctgcac caggtaatcg aagccccaga ccaggttgct ggaccgctcg agccggtaag
     8641 tcttcccctc ggcgctctgc caggacacga tgaggcccgc cgcgtcctgc gccgaaggaa
     8701 gaatcatgcc caggaaggaa gcgtcgtctg tcggatcgct ccccgccgtc aactcgtcgc
     8761 cgtccgtcaa ccgatcggcg tccgtgtcgg ccgacagcgg gtccgtgccc gtgttctccg
     8821 gcccggcgta gacgccggtc ccggtttcgt aagccgtctc gatgccgtcg ccgtcgtggt
     8881 cgacgcctgg gtaggcgtac tcgtatgcgc cgacgtccac gacgttcagg atgatccggt
     8941 ggttcccgtc cgggtctcgc gagccgatca tccagtcctg gttcgtcccc gcgttgatgc
     9001 acggagagtt gctcaagagc cggacgtcgc cggcggcata gtccgccatc ctcggcgcgg
     9061 cgatgaaatt gtccgtcccg tccggcatcg gggtgctgca gcagttggtc agccagccga
     9121 agtacgcatt caccgaggcg aaggcggtgt tgtagtagac gatgcagttg cgcggcgtgc
     9181 cccagaacgt gccgccgccg aacgaggtcg cgtcgttgcc gacgatggtg cagttgtagc
     9241 tctccgccga ggccacaccg ccgccgtaat cggccgtgtt gttcagtacg acgcagttgt
     9301 agatcgtccc acccgcggct ccaccgccct cgcccgcgtc gttgtggagc aggcggcaat
     9361 tgtgcagaac agaggcgcac gcgccccctc cgcccgccac ggccgtgttg ttggaaaata
     9421 cggtgtcgta cgcggacccg agatacagcc cggcgccgtt cgacgcggcg ttatggtgca
     9481 gcgatgaatc gttcaggagg ctatcgtacg cgccgccgcc gtaagccgcg ccgttccagc
     9541 ccgccgcgca gttcgagagg cttcccccgt acgccccacc gccgtaatcg gcccggttgc
     9601 tggtcaaggt gcaaccgacc aggtcaacgg cggaggcccc gcctccgaac gagggatggt
     9661 tcgtgaccga gttctggacg agcgcgcaat ccacgagcgt tcccccgtac gctccgccgc
     9721 cctcgccggt cgcgccggag accaggttgc ttagcagcgt gcactcggac aattgtgcat
     9781 tgtacgcgcc gccgccggcg ttggcggccg tgttgccgga cagcaagcaa ccagtcagcg
     9841 tgccgccgta tgcgccgccg ccgtcgccgt tggtcgcgct gttgcggctg atccggcagt
     9901 ccgacagcgt gccgtactcg gtcccgccgc cataactcgc ccggttgttg gtgacggtac
     9961 agccctccaa ctcggccgcg ctgaccccgc cgccacgcgc ggcggtgttg ccatcgaaca
    10021 cgcacgcctg gtagttgccg ccgtacccgc ccccgccgct cccggccgcg ccgcagccgg
    10081 cgacccagca attggtgacc acggccagcg aaccctcggc ccagattccg cccccctgct
    10141 tctccgacgt gccggtcgcc agggtgaatc cattggtcag cgtgaatcca tccaaccgcg
    10201 ccccgctgcc cagccagaca cagcgcaccg ccccggggcc gataccgccc tgccccgcga
    10261 tcgccgtgaa ggccggcccg ttgacgctgc ggacggtgac gacccggtcc acggcggcgc
    10321 ggttggtcag gccagcctcg accgcgcgcc cgccgttcgt gtagacgccg ttggtcaacc
    10381 agatcgtgca gccgaggctc gcggcgtcca cggccgcctg gatcgttgcg aaggcggtca
    10441 cccagtttgt tcccgccgcg ccatcatccc cgcccggccg gacatacagg ttgcagtccg
    10501 gctccacgat ggcgacttcc gtcgtcgcgg cgacggaacc ggcgagattg gacaccgtca
    10561 gcacgaccgg gaacactccc gccgccgcga aggcatggcc gaccacgcac ccgctggtcg
    10621 cgcgcgcgcc gtccccgaaa tcccactcgt agccctgcac ccggccggcg acaagcgcct
    10681 cgaacggcac cgagaacccg gcgggagcct gcgtgtaggc gcacgaaatc gccgcgctca
    10741 acgcgccagt acactccgat tcggaaacga attcgaacgc ccccatgtcc accgacgcgc
    10801 ccgtgatccg cggcgcgccg tcccagtcca cggcggctgc catccatggc tggttcgtgc
    10861 cccgtccgat gacggcggcg cccggcatga ggcgagggtc cagcgtgctg acgatgcccg
    10921 gcgggttggt gatgttgttg gcccccgcgg gccaggaagc gacgcagcag ttcgtaaagt
    10981 cgccttcgaa gaaatcgggg ccggtgggcg catcgttata gtagacgatg gaattcagga
    11041 gcgaaccgcg atacgctccg ccgccgctcg tgcccgcgct gttgccggcg accgtgcaac
    11101 tctccgacgc cccggctgcc gagtagagcc cgccgccata ttgcgcggcg cggttgccgc
    11161 gcacgacgcc gttccgggcc gctgaactgg caagcccgcc cccattcagc gcggcgttgt
    11221 ccgtcaccag gcaaccaaac aggaccgcgg aatacgcgcc gccgcccatg ttcgtggccg
    11281 cgttgcccag cagccggcag ttataaagag tgccccggca gcacccgccc ccgtagcggt
    11341 cggcggtgct gccggtgatc acgcaattgg tgatgaaggc gttcgtggtg cccgcgtaga
    11401 ccccgccgcc gctgcgctcg cgcgtcgtgt cgccttccgc cagcgtgtgg ccattggtca
    11461 ccgtgaaccc gtcgaggacg gtgttgctgg ccatgtagac gccacgcacg gccgcggcgc
    11521 cgtccgggac gccgtcggcg ttcggcgcgc cggcgatgat cgtcacgtcc ggcccgttga
    11581 cgctgcggag gaagagcggc ttgtcgagcg cgacgcggtt ggtcaagctg ccgtagacaa
    11641 cgcgcccgcc ccggtcgtag acgccgttgg tgacccagat cgtgcagccg ggccccgccg
    11701 cgtcggccgc cgcctggatc gtcgccttgg ccgtcgccca gttcgtgccg gccgcggcgt
    11761 cgtcgccgtt cgtcgccacg tagtaggcgt agtccgcgct gacgatttcc acggccgccg
    11821 tggcgacgac ggcgccggcg aggttggagg ccgtcagcgt gacctcgtac aggcccaccg
    11881 cggcgtaggt attggtgaca tacagctggt tcgtcgcgcc gcggccgtcg ccgaactgcc
    11941 acacaaaacc ctccgcgtta cccacgatcc ggccggccag cggaacggag tatccgacaa
    12001 ccgcctggag atcggcggca acgatctcca ccaagagcgg atcggagagg ccctggtatt
    12061 cgaaagcacc gatgtccacg ctggcgccca cgacgcgagc gttcaggtcg aaatcccgcg
    12121 cgtcggtcat ccagggcatg accagcccgg cgttgataca ggccgacgtg gccgaaaggc
    12181 gatagtcgcc gtccgggccg tccacgaacc ccggttcgtt cgtgatgctg ttcccgccga
    12241 ccgtcgtcgc ggggtagagg cagcagttag tgtaggacca gaaatacaca ttcgcgcccg
    12301 acggcgcgtc gttatccgcc aggatgctgc tgaagcaata actgtaggac actccgccac
    12361 cgcggttggt ggccgtgttc ccgatcacgg tgcagttgta caaccggctg taactcgcgc
    12421 cgccgccccc gtaggacgtg ttgccaacaa ggatgcaatt atagagctgg ctctcatcag
    12481 ccccgccgcc gctcgatcgg gcgcgattgc cggccagaat ggagcgatgg accgccgcat
    12541 cgaccgcgcc gcccccgtag tagtaagccg cgttgcccgt cagcacacag ttggagatca
    12601 tcccgtcccc ggaaacgtac accccgcccc cgctggtctt ggcgctgccg ctgtcttcca
    12661 gggcgagggt gtatccgttg gtcaccgtga atcccgccag caccgcgccg ccgctcaagt
    12721 aggccacgcg gatcgccccg ctcccgttcg cgccgttggt cggacagggt tggccgacaa
    12781 gaaacgtgaa gtccggcccg ttgacgctga tcacggtcac ggccttgttg atcacgacgc
    12841 ggttggtcat gccgctgtcc atggcgcgcc cgcccgtgtc gtagatcccg ttcgtgacga
    12901 ggatcgtctc gccggcggag gccgcgtcca cggccgtctg gatatcgtgc gcggcggtgg
    12961 cccagttggt gtacggggac gagtgactgc cggatgccgc tacgtatcgc gtggacgcgc
    13021 cggcccggac cgttacgcag accaagccgc cagccgcagc cagaacaggc aagatgcgca
    13081 tgagctcctt cgcgggaaaa gccccatgaa ccccagtttc aacgtacccg gccacgccat
    13141 aacggttaag cgagctcgcg caaaggaatg gacttgccca cccgtctctc gcggttaaag
    13201 tgagggagaa aggagtacgc catgcactca agccgcctat ggacgatcct tctgacaggc
    13261 ctcgggatta tgaccgcttc cgcccaggac aagctcccaa gcaacgtctc gttccggctg
    13321 aaggaggcgg accgcatcct ggcccgggcg gagcgcgccc tggaatccaa ctcggtggcg
    13381 tccgaggaat ggaagatcga gaccgcgaag accgccgccg tggaagcccg cgagaagatg
    13441 tccgagatcg agcagcggta cgcgggccag tactcccctt cgcaccccga catcgtcgcc
    13501 atgcagggcc gcatcgcgaa actggaggcc gcggccggcg ggcgggcgga cgcgatgcag
    13561 caggcgcagg cggcggccgg gcagcaggcc gccgccgccg gcgaggcctc ggagccctgg
    13621 ctcgcccgaa tgcgcccgca cgtcatcggc ctcgggcaat ccagccacga tccggacaag
    13681 tacctcgtgt ccagcgccac gcaggacccg gcggaaatgc aaaaacggct cgggatttat
    13741 gccgaggcct cggccgcgct ggcggaatac aagaaggccg gcctcggcga cctggccacg
    13801 gacgacctga aacaggtcgc cgctctcctc gagaacgccc tcgtccagtt tgaatcctcc
    13861 tgccgccagt acgccgagca ggacctccgc gacgcggacg cggccatcgg ccgcctggag
    13921 cagttcatcc gcgagcagga cgtcaacatg gcctcgaagg agaccttcct gttccccgag
    13981 cgcgaccaga tcggcaacgc gcaggagatc gtggaccgcg ccgccggcct gctgaagacc
    14041 ggcgaccagc ggatcaacgc gatgaaccgg cggatcgaaa acctcaagcg cgccgatgcc
    14101 cggctgcgcg ccgcccgcat cgccgagacc cggatggcgc cggacgccta cgccggcgcg
    14161 gacgcggcgg agttgaagaa gtccgccggg cagttcgtcc tgcgcgccca gcccggcgcg
    14221 cgggtcctca agacggcgat catcagcccg gactggaagc aggaaagcgc gttggaatgg
    14281 acggacacca cccagacggc cctgcgccac cggacgacgc gcagcctcac cgcgcaggtc
    14341 gccggcaagc gcaacgcgga cacgctgctc tacaccgtgg ccgtcagtca ggacctccag
    14401 gccgggggcg gctgggggcc gacctatggg cacatcatgt tcaccgaccc gatcctggag
    14461 gcgaacgtac cgtagcatga agcgcgcgct gtctttcggc ctggccttcc tgctgttcgg
    14521 gctcgtctgt cccgcgcggg cggaacccga cgtgacctgg gaggaaatcc tccagcgcct
    14581 ggacctggcc cggagcccgg aggccgtatt ccgggacgtg gtgcgcccac tgatcgaccg
    14641 gcccgcgacg ctggaggcca cgttcgagaa gtcggacacc gacgaggccg ggcgtccgcg
    14701 ccagcaattc cggcatgtca gcggccggca caccttcgtc tgctacacgg acgccgagtc
    14761 ggccccggcc gcgcccggct ggcacggcgc catcaccggc ctgctggtgg agttggattt
    14821 caaaggcgaa gacccgcagg gccgacgcta ttatatcctt cgcctgcgcc aggcggggac
    14881 aacgagacta ccttaagctg gaggatcgag ctcccgcgag accgcgccgc ggccgtcctc
    14941 aatacgcctc ggtccggaac accggctgga acagcttcgt gcggcgggcg cagaactcgc
    15001 ggaccgggca cgaggggcaa tccggctcgc gcatctcggg gcaccgtgtg cggttcatga
    15061 aaaagaaata gtcgatcgcc gcaaccgaca tgccggactg ctccaccagc cgcgccacgg
    15121 cgtggtaaac cgccgagcgg atatcctgct cggtgtccgc ggaaaccagg cggcgggcct
    15181 ccagatcggc gagcacgcgg gcgtcgtcga cgtcgaccaa ccccgtccgc agcgcgctgc
    15241 gctggaggtg gtaatcgatg atcggccggt ggttccaagg gtcggtgacg cgcaggaaat
    15301 gctccggcct gttttcgagg atgatcgcca gcaattgcag ctttttccgc atcgggtcct
    15361 cggcatagcc ggggaccttg ccggcctcgt ccaggaacgc cttcagcggc tccggggcgg
    15421 cattggccgc cgccacgagt tccgcgggcg ggcgcggcgc cgcgcgcagc cacgcggcgt
    15481 agcgctgaat gatctcccta tgttcgggcc acatcgggag cggattgacg cccgcgtcgt
    15541 cccggaagat ccccgcccag ccctcctcgc ccgcgcgcgc gagctcctcg cgggcgaaga
    15601 acagcggatc gcggttccac gcgcgcgtgg cgcatgcgac gatgaaatcc gagcccttgc
    15661 gcggtacgcc gtccacgggc gcgatcatgg gcccgccgta ccggcctccg tcaagatgcc
    15721 agaacccgaa ctggtgcgcg gtgacgaaga agaagtagtc gatcacgccc gcgcaaccgc
    15781 gaaccggaaa gagcgtcccc tccccggcgg agcagaaatc aaacggggag acctgaccga
    15841 tttccgcgaa gtattccgcg aaccgtcgga cccggacctc gttgacgcga acaggcatgg
    15901 cgtcttcagg gaacggcggg cgccgccacg acgaagtaga tcttcccgac gcgctccagg
    15961 cgggtcaccg cgaagccggc gtccgcgtag tgcttccgga tctgcgcggg cgtgaagaaa
    16021 tagacggggc agccctgcag tttcagccgg agccagcgga ccggcgcgcg ccacgtccag
    16081 accgtgggcc aggtggcgac gactttcttc cggacgctat ccttcatcct cttcaggaag
    16141 accagcggct cccggatgta gtcgaagagg ccgatggcca tgatggcgtc gaacggctcg
    16201 tcgaaccggg cctggaggaa atccccgacc tcaaaccggc atttatccgc cacgccctgt
    16261 tcccgcgcgt gtttccgggc cagctcgatc atggcgggcg agaaatccag gccgaccacc
    16321 cgctccttgc cgggcgcaag cgggaggcag aaccggccgg agccggtgcc gatgtccagg
    16381 atgctcttca gctccggcgc ggagcactcc tcgagggtca accgcatccg gtcgtacatg
    16441 tcccggcgga agacccggtc tagccagcgg gcgaacccgc ttttccggcc ggagtagatg
    16501 gaatcgaaat cgcccgccac gcggtcgaag taggcctgca cttccttttt ttccgcgtcc
    16561 gccatggccg atcctttcgt caccaggccg ccttgcggcc cgcgatatac ttgccggtcc
    16621 ccacgaagac cgcaacgata tttatgaaaa cgagcgcgac cacgctcaac ggattgaaat
    16681 tgcggaaatg gcggtccagc cacaggcccg ccgcgccggc gaggaccagc cccgcggccc
    16741 ccaggccgac ggaccacgcg acccacggcc ccagcacggc gctggacgcg aggaaaagga
    16801 cgagtacgaa cggcagcagc atgcgcagga gcttgtggga aacgaagacc cagaacaggt
    16861 agccgcgggc gggattcagc aacccgcgga ggtagcggat ctgctgccag ttcccgtagg
    16921 cgatgcgcac gcggcgccgg aataccgccg ccggccgggt cgacagccgg tccaccagtt
    16981 gcgcccccgg gatgtacagc acgcggcgac cgcgcagggc caggcgggcg ggctggacat
    17041 agtcctcgtt gatgaggccg gccgggaacg gctcgaattc cgccttgcgg aacgcgaaca
    17101 gcgagccggt gccgctcaac gtcgtccgga aaagaccctc ccagccgcgc agcttcatct
    17161 cgaagccgtg ataggaatgc tcggcggaat ccagctgcga acggtccgcg cggctcaccc
    17221 ggtagacccc cgccgcaccc cccacctccg ggtccgcgaa cggcgcgacc agctcccgca
    17281 ggccgccggg gttgaccatg ccgctggcgt cggaaatcac cacgagcgcg ccgcgcgccg
    17341 cggggatcgc gtcgttcagc acggccacct tcccgcggtt gaccgggaac gaccggcaac
    17401 gaatccgggg atcgccgacg gcggccacga ccgactcgct ggcgtccgtg cagccgtcga
    17461 gcgcgaaaat gaattcgatc cgatcagccg ggtaatccag cgccaggcag ttcgcgatct
    17521 tctcgccgag gatcctctcc tcgttgaaga ccgggatgac catgctcacc ggcggcaggg
    17581 ccgcgcccgg cccgatcgtc atccaggggc ggccacgcca ccgggccagg gccccgatca
    17641 tcaccgggta caggcagagc gtgtacccga ggacgaaccc gcttatcagg aagatcgcca
    17701 gcatacgcgc cctcgtcgcg gcctcacgcc atgtcccgga acggaacgaa ctcgaacgca
    17761 tccagcaaga catcgagccg gtgcaggagc gacttcatcc ccacccggtg ccgccagacg
    17821 cggagccagt gcgccggcgg gatcggctgc tcgggatcga tctcccaggg atgcagatag
    17881 acgacggccg acagcccctc ggcctcgatc cgccggatgg cccagcgggt cacgccgagc
    17941 ggatacagcc gcaggtatcc cccgcctgcg acgggaaggt tccggcccgc cacgcgcacc
    18001 gtcgagagag ggaattcgcg gatcatccgg ccgcccgcag ggacatcgaa gggcgcccgg
    18061 ggcgcgtccg gctcgccgta ccggtcgtgc atgacgggga acacggacga atcgtaaccg
    18121 atgcccgcct ccgccagcgc ctcccacgcc caggtgtcgg agcgacgcag ggagaaggac
    18181 ggcgcgcggt aaccgcggca ggacagcccg gtgatgtccc gcaccacacc gagcccgcgc
    18241 gccaggtctt cccggaaggc ctcgggcgtc aggcgatgca ccaggtcatg cgcgtagccg
    18301 tgggaggcca gttcgtggcc gcgcgcgacg atgtccttca ggaggtccgg atgccgttcg
    18361 cccacccagc ccaggatgaa aaacgtagcc cgggcttgcc gccggtcgaa caggtcgagc
    18421 agcgcccggg tctgcgcctc gatccggctt tccctcgcgg cccagctttc gcgcgggatc
    18481 gccttctcga agttggccac gcagaaccac tcctccaaat ccaccgaaag aatatgcttc
    18541 atgacgggtc cgaatccgac gggcacggtg ccagcaaaag acgcgaaaaa caaggagatt
    18601 aaggggaatc cggccgatcc aactccgggt tgttcggccg ggtcaggacc aggtgcaggt
    18661 cgttgatcac ccgcgcggcg aagccggcct cgcagtaggc caggtagtac tcccacttcc
    18721 gcaggaacac gtcgtcgaag cccagcgccc ggacctgctc tttcgaggcc aggaaccgct
    18781 cccgccactc gcgcagcgtg cgggcgtagt gcgggccgat gttttccagg tgctcgacga
    18841 cgaaccgcga gtgccgggtc atcgcgcggg agagcgcggt cagcgacggc aggacgccgc
    18901 ccgggaagat gtgtttctgg gtccagtccg tcgcgcgtcg gtacatgtca tagcgctggt
    18961 cggggatcgt gatcacctgc agcacgacca ggccgtccgg tttcagcagt cggtcgcacg
    19021 cgcggaagaa agcgccgaag tactcgtggc ccacggcctc gagcatctcg atggagacga
    19081 tgcgatcgta gcgccccttc acgtggcggt aatcgcatag ctcgaccgag atccggtccg
    19141 ccaaccccgc ctcgcggacc cgctgccggg ccagttcgag ctgctgcgtc gaaagcgtga
    19201 tcgtcgtcac gcggcacccg gtggcgcgcg cggcctcgat cgcgaacgaa ccccagccgg
    19261 agccgatctc caggatgtgg tgctccgggc ggatgcgggc cttgcggatg aggagccgga
    19321 gccggttcat ttgggcgtcg tacagcgatt gagccggcga ctcgaaaata gcgcacgaat
    19381 acatcatcgt ctcatccagc cacagccgga agaaatcgtt gctcaaatcg tagtgatcgc
    19441 ggatgttccg ccggctcacc cgcacggtgt tacgccgcaa ggagtgtcga agccggttga
    19501 tgctgcgccc gagccacgaa agcgcgatct tccggtcgtc cacggaagcg agattgtcgg
    19561 ccagcacccg cagcaggccc ggcaggtcgg gcgtcgtcca ttcccccgcc atgaacgact
    19621 cgccgaaccc gatgtcgcca tccaggatca gccgccggaa gaactcgcgg gattgaatgc
    19681 gcagcaccgc ggtggacgcc gccgggtcgc ccgcggttac ggtcttcccg tccgggagcg
    19741 ccagctccag gcggcccttg cgcacctgcg agaagaaccg gaccgccagc cgttcataca
    19801 aacgctccag ccgcgtcggc gccgcgcgcc ggatggtcat cgaatggacc ggctccggcc
    19861 ggtgatgcac cttgagtttc ttgcggtaat aaagtttggc cgcctcccac acgatccggg
    19921 gcatgacgag aaatacgttg agcggatagt tccgcagcgt ccgcttcagg ctggcccggt
    19981 ccagagggat cccctcgccc ttcatcgcgg cctgcagcaa agggcggccg tcgtccgtct
    20041 ccaggtccac ccggatgtcc agcgccgtcc ggatgtccgc aaaggaaaac cggtagcgac
    20101 cgctgatgtc gttgaacggg gagacgtgga attccttggg gtgcatgaag ccaacaggcc
    20161 acgccgttac cggcgcggct tcttcgccct tcagcacata caagtgccgg tcgccaaacg
    20221 tgttgttcac ctccgccacg gcgcagcgga ggctgccgtc ggcacgcagg gcgtagtaga
    20281 agctcaccgg gttgaagaca tagcccagca gccgcgcgga cgtaacgaga atgacccgct
    20341 cgatcccgtc ggcgcagccc tccgccccga ggcggcgcag cagcttctcg cggatcgtgc
    20401 ccggccccgc gtcgagatag tccgcgtcgc ggatggagac cagccgccgg cggttgtatc
    20461 cgaagaacgg gatctgtcgg tcgagttccg gcagctcgtc gaggtccagc gcgacccagt
    20521 agcccgggaa ctcgaactcg tgcttcaccg gctcgtgccg ggcgtgccag accgtgcccg
    20581 tgtagatcct cgaattcaca gctcgatccc gaactgccgg gccacggcgg cgccggactt
    20641 cacggcgtcc tcgtggaacc cgtagctgaa gtagctcccg cagaaaaagg tgttccggcg
    20701 cccattgaga tcggccagct cgcgttgcgt gtccatcgac gccgccgtgt acatcgggtg
    20761 cgtgtcgtcg aacgccgcga gccgcgctgc cgccgggatc ggttgccgcc ggttgagcga
    20821 aacgaagtac tcgcctcgcg tcttcaacga ctggagccgg ttcatccagt acgtcagcga
    20881 ggccggcgcg tccggcggcg cgccgcgttc gcgaatgtaa ttccaggacg cccaggcgtc
    20941 gcgcgccggc ggcatggcgg ccgcgtccgt atgcagcacg gtgtcgttgc gggaatagct
    21001 ccacgggccc agcaggcgct gctcggcgcc gtccgggtcg gcgagcagct tcaaggcctc
    21061 gtccgcgtgc gcggcgatga ccacgcggtc gaaccgccgc gcctcgccgt tttccagccg
    21121 cactgtcacg ccgtccggct cccgcttcac ggaagccgcc gccgcccgcg catggaatgt
    21181 cccgcggaag ccggcctgga tcttcttcac gtacgcgtgg ctgccgccga ccagcgtctt
    21241 ccactgcggc cggttgctca attccagcag gccgtggttg cggaagaact ggaggaagcc
    21301 ggccgcggga aagtccatca tctgcccggg cggcgtggac cagatcgccg cgcccatcgg
    21361 caggatgtga tcccgtatga aggactccga gtacccgccg cgccgcaggt actcgcccag
    21421 cgaaaggccg gcgtccagcc cccgcgacag gtcgcgcagg ccactccgat agaagcgcag
    21481 gatgtcggcc acgagccgcc agaagcgcgg gctgacgagg ttgcgccggc gcgcgaacag
    21541 gccgcgccac aggtcggagg aataacactg ccccgtgttc tcgtcggtga acccgaacgc
    21601 catgtcgctg ttttgcagcg gcacgccgag ctggtccagc atccgcgtga gcgtcgggta
    21661 gttccggtcg ttcatcacga tgaagccggt gtccacgggg gcgccggcgt cggggccgtc
    21721 cttcaggacg atcgtccgca catggccgcc gaggtagtcg ttcttctcga agaccgtcac
    21781 gcggtgcgcg cggtccagca gccacgccgc cgtaagcccg gccccacccg ccccgatcac
    21841 cgcgatgtcc agcggcgcgc tcatcccgcc gcccccggct tgcgcagcag gtaatgcgag
    21901 acgccccacc cctcgccgcc gcggtagccg aacagctccg cgcaggccag gatgaagatc
    21961 ctccagcgct gcagccagcg cctcttctcc gcggcgccgt aggccttctc caggatggcg
    22021 agcgcctccg ggcgcgcggc gtcgagttgc tcgagccacg cctcgagcgt tcgcgcgtag
    22081 tggcggccgt tgacctgcca gtgctcctcg atccgcaggt cgcgctggaa gtagagcatc
    22141 agcgaatccg agggcatgat cccgcccgtg aagaagtagc gcgccatcca gtcggtcgcg
    22201 tcgccggtct cgaagggata ggccagctcg cggtgcgtga agatgtgcac gaacatccgg
    22261 gcgtcgggct tcatccagcg cgcgacgcgg ccgagcagtt cctcccagtt gcggatgtgc
    22321 tcgaacatct cgaccgtcac cacgcggtcg aaaagtaggt ccgcctggaa ctcgttcatg
    22381 tccgccgtga tcacccggac gttgtcgagc cggcgccggc cgcactcggc ctggatgaac
    22441 tcccgctggc cgtgggaatt cgacaccgcc gtaatccgcg cgccgggaaa atgcccggcc
    22501 atccacagcg tcagcgagcc ccagccgcag ccgagttcga ggatctcctg cccgtccgcc
    22561 agccgcgcgc ggcgcgcgga gagatcgagc atggcctcct cggacgccgc gagcgtgttc
    22621 acgccgtcgg gccagatccc gctgctgtat ttccggcgcg ggccgaggat cagctggaag
    22681 aactccgcgg gaagctcgta gtgctgctcg ttcgccttgt cggtggccac ggcgaccggg
    22741 ctccggcgca tatgctggat gaaggactcg tgccggacgc ggatcgcctc gacgccgccc
    22801 gccccttcca tgcgcaggcg ctcggcgtgc agccggcgga tgccggcccg gatcagcgcg
    22861 tcggggacca ggccccgctc catccagtca atgacccgct tcatggggat gcgctccttt
    22921 tcgggaacca tgggaaaaac gcgttcgtcg tgcgctggta ctcgcggtac tcctcgcccc
    22981 ggctggccag cgcgtgcgcc tccgtcgccg ggatgccggt gaccttcagc agcacgtaga
    23041 gcatcagcgc cggcgcgatc agcgtcagcc agaacccgcc ccacccgacc gccaacagaa
    23101 catagaccca ccagtggagc cactcgaaaa agtagttcgg gtggcgcgaa tagcgccaca
    23161 ggcccgagcg gcaggtccgg cccttgtttt ccggccgccg ccggaaccgc gcgagctgcg
    23221 cgtcggccag cgcctcgccg ccgacggaca ggatccaaat cgcgatgccc gccgccgtcc
    23281 agggtccgac gagcgaagcc gggttgcgca tcgccgccca cggggacagg gacaggatga
    23341 tcaccgtgac ggcctggaac tggtagaaat tgaacagccg gcgctggaag ttcgcgcccc
    23401 attcctgccg caactggcgg taacgcccgt cctccggctt gccgtgattc cgcccgtgga
    23461 tgtaggccgc gagccggaag ccccacccgc aggcgagcgc agccaccagc accccgcgcg
    23521 ccacgtcgcc gtccagggcg gccgcgtaga aaaccgagac cagcccgatc cccgccgccc
    23581 agccgacatc cacgatcccc gcgtcgcccg tccgccgctg caccagccac agcagcccga
    23641 agaaggccac catcgccgcc cagcccgccg ggatgaccca cgcgctcgtc atcgcgctcg
    23701 cgcctcctcg tacactttcg ccggcggctc gcgcaggtcc cagaccacgc cgatcgcggc
    23761 cagcgcgcgc agggtgtagt aggtcaggtc cacctcccac cagaaaaatc cctggcgcgc
    23821 ggccacggcg tagcggtggt ggttgttgtg ccagccctcg cccatgctca agagcgccaa
    23881 cacggcgttg ttccggctcg tgtccggcgt cgcgtagcgg cgcgagccga ccatgtgcat
    23941 gagcgagttg atgcaaaacg tcacgtggta gaccaggacc gtgctgatga agaaacccca
    24001 gatgacgaac tgcgggccgt tcgccccgcc cgccggcgcc agcccctcgc cgagtatata
    24061 taggaataga accagcgcgg cgggcgcgac gaacggatgc cggtccagaa agcgaagttc
    24121 aggatatttc gcgaaatccc cgatcaggtc atagcgggtc ctggaatagt cgggaaccag
    24181 cagccagccc aggtgcgccc agaagaaacc ccggcgcgtc ggcgagtgga tatcgtcctc
    24241 cgtgtcggaa aagcggtggt gatgccggtg gtgcgaggcc caccagagcg gcccgcgctg
    24301 cgccgccgcc gcgcccagca gggccaggac gaactggaag gcccggctgg ttttgaaagc
    24361 attgtgggag aagtagcgat ggtatccgcc ggtcagggca aagacccgga ccgtgtacat
    24421 cgccacgcag gccgccacgg ccgcccggct caccccggtc cagaaaaccg ccaggcacgc
    24481 cagatgcatg aacagcagcg gcagcccttc tttccaatcc aatcgccgca ggatggatga
    24541 tgcgcctgtc accttatgaa cacccattct ttttatccag taatcattag gcgggcaagc
    24601 ccttgaatga cgtcaggcct caacggggag cttcttgccc cggtcccgga ccacgagcac
    24661 gcccatcatg atccggtaag tcagccaggc cagcggcacg gccgccagca gccaacctgt
    24721 aaccgcgcac aacacggcgg cctggaagga ctgaatgaat tcccacggat gttccttcag
    24781 cctcgccgcc agttccgcgg gtgacagcgg cagcggctcc actcgaaaca cccattcccc
    24841 gagccgcagg aagggccaga tcaacgccaa ttgcacgccg ccgaacaacc agttgaccgc
    24901 ctggatcgcc ggcaggttca gtttcagccg gaccgcgacc accgcgcaaa gaaatgtcgt
    24961 ggtgcccaac accgggaata cgctgataac cgctcccaag accaggcatt gcgcgatttt
    25021 ttccggcgta accccctgcc ggaagagagg caacagcgcc ccccccacct tccggcggaa
    25081 atatccatcc ggcctgtttt ccctggcttc gctttcctcc atgtccggct ccaagtaagg
    25141 tcccgcaata tgcccgaaaa gcttgaattt tcaactggaa cctcattttt tcagaccgcg
    25201 cccttttttt aagaaaaagt gcggaatacc tccttcctct ttcctttcta ttaatgaacc
    25261 aggaggagga acaaatgagc acaagaagca agaaagtgat cgtggcggtg ttgatcctgt
    25321 tggcgtcggt tctatatggc tacaccatgc tcacgcggca gtcgccgtgg gaggacgggg
    25381 tgttctacga ggtcgcctcg gatgtgatca gtctcgtgta ttaccgggcc gaccaggaca
    25441 cgctgaccct cgcctttcgg agcgggccgg tctacgagta cagttcggtg ccggcctccg
    25501 tgttttacgg cctcatggat tcgcaccgga aaggctcgta ttacaaccag cgcatccgtg
    25561 gtcagttccc gtcgcggcgg cttgaactct cggcgcccgg cgaggaaatt tgaccgggtt
    25621 gaaccggcgc ccgctcggcg cgcgcccggg tcgcctcgta tcgcgcgggc gggcagggtt
    25681 ccgcagccat catggcgcca acaacggggc tgggtccgga acgcgcggcg gatacaggtg
    25741 gcaatgcacc cgccgggtct ccgtggcgga agccggggga tagtccaggc ggcactgatc
    25801 catggccttc ggacagcgcg catggaaagg acagccggac ggcggattca ggggagaggg
    25861 aggctcgccg ggcaacacca gtttccggcc ccggggctga cccggcgcca ggaccgcggc
    25921 cagaagggct tgagtataag gatgcaaagg cgagcccatc acggtttccg cgggcccctc
    25981 ttccacgatc cggccgaggt acatcacggc aatgcggtcg ctcaagtggc gcaccacgct
    26041 caagtcgtga gagatgaaca ggtacgacag ccggtgcttc tcgcgcaggt ccatgagcag
    26101 gttgatgacc tgcgcctgga cggacacatc gagcgaacta accggttcat cgcatacgac
    26161 cagggccggg cgcagcgata tcgcccgggc caggctgatg cgctggcgct ggccgccgga
    26221 gaactcgaag ggataacgat acagggcctc cggcggaagt ccgacctcgg ccagcagccg
    26281 ggcgccttca gcctcccgcg agccctccag aaggccatgc tcctgcagcc cttcggtcag
    26341 cgcgtccagc acggtcagtc gcggattcag cgaggcggcg gggtcctgga atatcatctg
    26401 gaggtgccgg gcggcgcgct tgcgttcggc gcgggagagg accttcatgt cggtgccctg
    26461 gaacagaatc tgccccgacc ggggccgctc caggccggca atactccgtc caagcgtcgt
    26521 ttttccgcag cccgattccc ccaccaggcc gagcgtttcg cctcccatga catgaagcga
    26581 tacgccgtcc acggccttga cgtggccgac caggcggttg agcaaacccc gccgcaccgg
    26641 aaaccacgtc cgcaggtcgc ggatttcgag caatggagcg ttccccgtca cgggccgatc
    26701 cccgcctcgt ccagcagaaa acacgccgcg ccgcggcctt caccacgggt gtacaaaggc
    26761 ggctccgcta cgcggcaccg cccgaagact ttcggacaac gatcggagaa gtggcatccc
    26821 gtcgggtaat tcagcggcga cgggacctgg cccggaatgg cttccagccg cccgcccggg
    26881 gcctgcacgg acggcatgga gcgcaggagt ccctgcgtgt acggatgcaa cggcgcccga
    26941 aacagcgcct ccgccggccc cgcctccacg acgcgggacg catacatgac caccacccgg
    27001 gagcacatgg cccagacggc gcccatgtca tgggtgatca gcaggaccga cgtgctccgg
    27061 tccttcatat cgagcagcag gttgaagatc tgggcctgga tggtgacgtc cagcgccgtg
    27121 gtgggttcgt cggcgatgat cagcgcgggc tccagcatga gcgccatggc cagcatgacg
    27181 cgttgccgca tcccgccgga aagctggtgc ggccaggcat ccatgcgctc ggccgccgcg
    27241 ggaatgccga ctttcttcag ccactccacg gccaggctcc gggcagcccg ccgcccgatc
    27301 ttctggtgca actgcaacgt ctcgaccagt tgatcgccga cccggtgcag gggcgacagc
    27361 gccgtcatgg gatcctggaa gaccatgctg atggcctggc cgcgaacgcg ccgcaattcg
    27421 ctcgcaggca gcgcaagaag gtcccgcccg cggaacagga tggagcccga ctcgatgcgg
    27481 ccgggcggcg acgggatgag ccgcaggatg ctcaaggccg tgacggtctt gccgcagccg
    27541 gactcgccca ccaggcccag cacctcgccc tcctcgatgt cgaacgacac ccggtccgcc
    27601 gcgcacaacc ggccctcgtc ggtttcgaag gcaaccgtta agtcctgtat ctgcagcaag
    27661 gcgcccatga gtcccgcggc gcattgtcac agatcgccgc caacctttga atcgaaaaag
    27721 aagtcggatc gccgggtcta ctcgagggat accttgagcc ggtagtaacg cttcaacacg
    27781 tcgggcgcga tcgcgttggt cgacgacgcc ggaccggtct ggccggcgcg ccaattctcg
    27841 atgaccggct gctcgaccga accagccagg ttggtgaggt agtagaccga gtagtaccgg
    27901 cccgagatcg ccggccaggc cacgaccacc tggttgccga ccggcgcggc aggcgccagt
    27961 tccaggccgg aatccggatc cgtcggatcg gtcccggcca cgtattcctt ccacgccgac
    28021 agcccgtctt cgtccgtatc ggtcaactcg gcggcatcgt agtcgttggt cgccgggtag
    28081 aacgccgcca gccacggttc cggagttccg caagccgcca tgatctccac gaaagacgcg
    28141 agaacctgtc gcgggcggtc gagggaaacg gtcatgacgg ttaaattggt atcgccctgc
    28201 gtgtcgccgc cccagccagc aaagcagtaa tgctcggccg gcgccgcggt cacggtgacg
    28261 ctcgcgccga acgggtacca gtcgctggcc gggcccagga ccgcgccgtt cgtccccgcc
    28321 gtccgggcga agtagaagtt cgttgtccag cgccaggtca gcgtcgagtt gttggtcaac
    28381 gagaaggatg tgttggtcaa cgagccgctg gagggcacgc tgcccgtccc cgtccaccct
    28441 gcgcagacat actgtgtctg gccgctcacc cacggcgacc cgcccaccgc ggccgagacc
    28501 caggtcccgc tggtaaacca gtttaccccg actacaggat caggattgcc gcacaaggag
    28561 gaaactatca actgattggt cgcggccggc tcgggcagcg tcacattcgc cacggaggaa
    28621 tacacgccgg tgccgcccgg agacaccgcc cgcgcccgga agtagtacgt ggcgctcggc
    28681 gttagccccg tgaccgcctg gcttgtgccc gaaacaacac gattcgagta gtccggaaga
    28741 tagcacgggc ccgcgctctc catgcgcgtg acctgcacct cgtcgatata gcaactgtct
    28801 tgcgatcgcg cggtcatgta ccatcgcacg tattgcgtcc ccgccaggtt cagggggatg
    28861 ttggtctggt agggcgtgtt gtcgatggtc ccgcctgtcc cgctttcgac aatgctcgcg
    28921 cgatcgatcc agtcggtccc gttggaggat gtctggacga cggccgtcca gttgccggga
    28981 tccgtcgagg tccggatata gaaactcaac accccgggat tggtgcaggc cggggccagg
    29041 atccagtcgc ccgcgccgtt catgccgacg caacgcgaat tcgtcacccc tcccgaggag
    29101 tactgggcgg cgctgttttg agtccagccg ttggtccaag tggatccgtt ccacgcctgg
    29161 aaatcctcgt tcagcagcac gcctccaccc agatccacga aatcctcgca catgcccgcg
    29221 tccagccgat aactcgttgc gcccggcacc gccgtccacg cggccgtgaa gccggcggcg
    29281 ttggtctcgc tcgcccagat cgcggccggc gccgcgggcg gcccctcgac cacggtcacg
    29341 cccacgacct gcgtagcgac ccccgcgctg ttgctggccg tgaaggtgaa gctccgcgcc
    29401 ccgacgtccg ccagcggcgg cgtgtaattc aactccccgg tcgccggggt gaagctgtag
    29461 cccgacgagg ccgtggcgct ccgcagcgcc aggaccggcg ccgggtagcc ggacgcgctg
    29521 accgtgaagg ccagggccac gcccgtcgtc gccgtctgcg tcccccgggg atcgaacacc
    29581 ggcgcctcct cacccaggac ggccacctgg ccggtcagcg cgtaagcgcc gaggcttccg
    29641 taagccgtgt acccgctgcg cgtcgtgttc gtcggggcgc cggcgtcgga cgggtagacg
    29701 tgggcgtaat acgtgccgta cgtcaccgac tgggtgatgg aggcttgggc cgcggccgcg
    29761 atgttgttcg aggccagcac cgtcccgttc gaggagcaca gcagcagcac caggtcgctg
    29821 ttgctgcccc aggtgccgtt ggtgcagcgg tacgggatca cggacagcgc cacgacgccc
    29881 gcgccggacg tcatggaata cacgtctcga tccgaggtcc gccccaccag gccggtcgcc
    29941 gttacgctca acccgtcgac ggtcaccggc gtcgccgatg cgctgttgct cccgtggtca
    30001 tccgttttgt aagtcagctt ggccgagatg atcgccaggt catcttcggg ctggttggca
    30061 tcgtagtatt cccccttgct ccattgcgag aggttgcgcc cgtaacccgt gcccatgatc
    30121 gggccccacg aggccgcgtt ggtggtcgcg gcgtggccgt tgtagtactc ttgcgtcgaa
    30181 gtgccgtcat ggctcaagcc catgttatgt cccagttcgt gcgcggccgc ctcggccgtg
    30241 ttggcgtagg aatcgccgga catgatcgtg atccaggccg gggagtagca tccggcggcg
    30301 ttgtaggaat agctggcgta gttgaacaca tccacgtacg caatgccgcc gtagccgtag
    30361 tgcgggcagc gctttccgcc ggcgtccgtg gtgggcgtga tgagcgcgtg ccccgttcgt
    30421 cggtgccaca aagggggctg gacggtggtg acgttgatgt cgaacggcgc gtaatcctcg
    30481 gccacccgct cccagatctg cctgatgtag cgctgctcga ggtcgctgaa cgtggtgttg
    30541 tctccgtcgg tgtcgaacgg cgtggtgttc cacacggtct gggtataggc cgtattccac
    30601 atcgtgttgg aaaccacgtg cccgttgaag tccaggaaca gcacgttcgt cgagcccggc
    30661 ttgctgctgt aggccggcgg gttggtcacg gaaatggacg ccagaatatc tctcgttgaa
    30721 tcgagcgaca tcgcgccggg ttccgcgctc cgtcccggct cggcggccgg catgccgccg
    30781 aaatcgcaga cgtagaagat gccgccgttc cggtccgccc gcatggatcg taggtcgttg
    30841 cggtgaaact ggatcgactc cagcttggcc agcgcgcgcc cccgggcgtc catgggcagg
    30901 gattccagtt ggccgcggag aacgcccgca ggaaggtctt cgactcggac gccgcgcccg
    30961 ccgccgaaaa caccgtggag atccggtccg tattccctga cctgggcacc ggcccgacca
    31021 cagatcagca gaaaaacaag cagaccggcg accgatcggc cgatgcacaa acgccccacc
    31081 tgccctctgg agaggttatt cacggctcgg tcatgctctt cgcttcagaa agagcagatt
    31141 ccgggccaat gggttcggca caccggagga aagacctcta ttccaggcgg gaatatcgtc
    31201 ttggatcgaa ggcggcgcgg acgccttccc caatgaatac accaagcagc ataacgataa
    31261 acaaggctag agacgggtat agcgacaacc accatgccca gttgaactcc tgggcttggg
    31321 cgagcaactc accccagctg ggtgtcggcg gcggcatgcc gaaaccaagg taatccaatg
    31381 ccgccaggac gccgaccgcg ccgaccaggg aaaacggaaa gaacgtgacc acaggcacca
    31441 gggcgttcgg caggatgtgc cgaaagatga ttttccacgc cggcaggccc atgcaccagg
    31501 ccgcctcgac aaacgcctgc ttgcggagcc ggaggaattc ggcccgcatg taataggaaa
    31561 tgccgatcca gttgaaaatc ccgtagcaaa ttagcaacag tgcgaagctg cgtccgaaca
    31621 ccgaacccat caggatcagg atgtagagga agggcagcga ctcccagatc tcgatcgtgc
    31681 gctggcccaa caggtccacc catccgccgt agtatccctg gacggccccg accacgaccc
    31741 cgaccagcat cgtcacgacc acgagaagga ggccgaatgt caacgacgtg cgcaggctgt
    31801 acaggatgcg caccaggaca tcgcggccgg cgctgtccaa cccgagggga tgtcccgggc
    31861 acggccgcca gggataggtg accgattctt tatcaaaggc cgcgcggagc catcgctccc
    31921 cttcctgcca gcgctcctcc gacacaggcc cctcaaagcg cgaccgcgcc ttgtccagca
    31981 gtatccgctg ccgtgaagcg tcgaggtcgc tccacaaggg aggacggccc gtcgccacgc
    32041 gcccgtccct gtccacccgc agtttctcgg tcgccgcgcg accggcgccg gcctcgcgca
    32101 ggagcatgcg cacggagccc ggcgcctcgc gtctcggcgt gtaggtcgac agcgaaagca
    32161 gccccgtgcc cgccttgcgc agcggcgcct ccgccgacac ggacgcggcg gcccggttct
    32221 ggaaccgcgc gcgaagcccg tcgcggacgg cggcaggaag ctccatgaag aagtcgatat
    32281 ttcttccggc catatccgcg tcggggacac cgaggaagaa cgcgcagttt tcggcggaca
    32341 ggatcgcgcc gtcggacgac aggctgacgg cccccacgac ggcctcccgg gtcagcgtca
    32401 gcaggacttg atcgggcaag gcaatggaat ccgggcgcac ggtctccagc gggccgaacg
    32461 gaatcagggg gaagaccatg cggcggccgg ggtcgctccg gaacgcttcg ctgccggcaa
    32521 tctgcttgta gtccggccgg gtcatgcgcc cgctgccggt gaagacatcc tcgggataga
    32581 aacgaagcag cgggaaatag gatcgccccc ggtagcgcat gtacaggggt tggttgccgg
    32641 cgaccagttc ggagaccagg ctgatcgcgt agagaacgat gaggatccag aacgatcccc
    32701 acgcgcgccg catatcgcgg aaccgctgga gccgtttccg ggtgaccgga ttcaggcccc
    32761 accgccggcg ccgctcctgg acgtccgtcg tcatgactgg ttccggaacg agatgcgcgg
    32821 atcgatcagc agatagcaga aatcggagag caggcggccg agcagaccga ggatcgccgt
    32881 ggccgagagg atgcccatga agaccatgta atcgcggctg acgatggcat cgaggctcaa
    32941 tcggcccatg cccgggatct cgaaaacgcg ctcgatcagg acggaacccg cgaacataac
    33001 ggtcaagatg ccgccgaatc ccgtggcgat ggggatcagc gcgttgcgga aggcgtgtcc
    33061 ccagatggcg cgggcgcgtg tggcgccgcg ggccaggacg gtacggatgt aatcctggcc
    33121 gatctgctcg agcagcgagt tcttcatcag cagcgtcagc accgcgaagt tgccgaccat
    33181 gtagcagagc accggcagga acatgtgctg gaacaggtcc ttcgccttcg cgcccaggct
    33241 catggcggcg aaccccgccg agtggaagcc ggaaaccggg aagaggtccc agaacgtttc
    33301 cgtgacgccg cagaacagca tcttcaacac catgccgaag gcgaacgggg ggatggcgta
    33361 gccgatgaag acgataaggc tggaggccag gtcgaatgcg ccgccgtggc gcagggcctt
    33421 ggcaatgccc agcgggatac acacgagata cgtcagcaca aatcccgtga tcccgaacac
    33481 cagcgacacg gggaaccgct tcacgatcaa ctgccagacg gtctggttcg ggtacttgta
    33541 tgactcgacg gccagcccga tccggtcatg cacgagccat ttccagtagc gttgaggcag
    33601 gggcttgtcg aaaccgaagt gcttttcaag ggccttccgc tgttcctccg tgatcggccc
    33661 ggccgcggag gacatgcgtt cgccgcccgc acccaacccg cgcatctgca ggatgacctg
    33721 ctcgaccggc ccgcccggca cgaactggca caatccgaaa ctgaagaacg tgatcccgat
    33781 gaacgtcggg atcatcagga gcagtcggcg gaggatgtaa tccaggcgat ccaaggcggg
    33841 gaggattcag gggttagggt tcaggggctc gggagcgact tcctgcttat catcgccttc
    33901 tccctgcgga gccttggcgt ccttcgcgtc ttccgttcta attccggttc ctggcgcctc
    33961 ccggaaaacc tcgtcgaacc ggacatccgc cggcttgcgc ggcaggggga ggccgtcctg
    34021 catcgcgtcc ttcaattcgg ccgccgcgtc ctcgtcgtac caccagtacc agtaggccgc
    34081 gcgctcgtcg ccgtacttgc ccagcaccgt cggcggggcg ccgaacctgt tccagtacag
    34141 caggcgcgtg tagttgatgt tccagagcag gatgtacggg gcctcggcaa ccaggaggcg
    34201 atcgatgcga cggcagatgt cgtgccgctg cgcgatgtcg aaattaccgc gctggctggc
    34261 gatcagggcg tccacctccg cgttgcggaa ccccgcgatg ttctgacccg accgcttgtc
    34321 cgcctcggtc gaggaccaca tgggctcggg gtccttgcgc agtccgccgc tccacgcagc
    34381 ccaggtcatg tcgtagttga actcgtccat gtccttcgcc cacgcggccc agtccttctt
    34441 ctcgatctcc atttccacgc cggcgtcctt cagggcctcc tggtagatca cgaggaactt
    34501 gtccgtcgcc gcgtcccggg acaggaaacg gaaacggagc ggcttcccgt ccttctcccg
    34561 caagccgctc ttgggattga ccgtccagcc ggcctcggcc agcagctcgc gggcccgctc
    34621 cctgtcgaag cggatcggct tgttttcgca cgggtggtcc gcgccgtaca ggtcctcgaa
    34681 gtacgagcgg tgcaggaagt actggttgtg catcagggtc gcgttcatcg tttcgcggtc
    34741 gaggaggtgc gccagcgccc ggcggacccg gatgtcgtcg aagggcgggc gccggccgtt
    34801 catcgcgaag ccctggaacc cgatggggtt gtaattgaag acccgctgct tgatcaccca
    34861 gtttttctcg tagcgctcgc cctccgtctg cgtcgcccac aggtgcgccg tgtagacggg
    34921 aaagaggtcg atctcgccct tcttgaacgc ctcgaacgcg ttatcgcgct cggcgaaaaa
    34981 tttgaacacc agggtctgga agttgccgac gccccgggcc cgcgccgagc cccgggtcca
    35041 ccagtcctcg cgccgctcca gcttcgccag cacgccttcc tggatctcgc ccagccggta
    35101 ggacccggag acgaccggga actcgaaatt gatcttgttg aagtccgcgt cggcgaacgc
    35161 atggcggggc aggatctgga agctggccag cgcatccagg ttctgccagt ggatctcctt
    35221 tgcgtggaac cggatcgtgc gctcgtcgag cgcctccggc gactcgaagc ggccgaggaa
    35281 aatcttgtgc gggccggtga tgttctccgg cttcatgacg gcatcgaagg tccatttcac
    35341 gtcttccgcc gtgaccggcc ggccgtcgct ccacgccgcg cgcggatcga tccagaaagt
    35401 aaacgtgcgc ttgtcctcgg agatggccca ccgttcggcg agcccgggct cgtagtcgag
    35461 cgtcagcggg tgctggccca gcagcgtctc gtacatcgcc ccgaacagtt cggccgagaa
    35521 cacgttctgg tcgaggtaat agttgaagct cttcggatag tcgccggcgt agatcgcgat
    35581 ctcgccgccc ggcacggcct ccgggctggc caggggattg ggcgcatcct tccagccgtc
    35641 gcctggaaac agcgcgtcgg acccgggaac cgccatggac gccagcagaa ggaacgcgga
    35701 ggtgattagg attattttca tgggaggttc agaaggattc agggttcagg atcttccaca
    35761 gcatagtatt tttccatgcc gtacgggaag tcgtggtatt cgaagttccg tgcccagggc
    35821 tggagcagga cgcaggccgc cggatgatgc gtgaagatcc aggggcagtc ctcctggatg
    35881 atcgaggcca ttcgccggta tagcgcggtc cgctcgtccg agtccggcat cacgcgcgcc
    35941 tgttcgcaca gccggtcgta ttcggggttg cggtaattgc tgcggttgga gcccggcgag
    36001 gcgttgggac tgtagaatag ctgcaggaag ttctcggcgt ccgggtagtc ggcgatccag
    36061 ctcaaccgga acatctggac ctggcgccgc tcgatccgcc cgagaaacgt cggccagttg
    36121 ttgtaccgcg gctcgatcac cacgccgatc cggtccatga actggatgaa aagatcggtc
    36181 gattggcgca tctccggatt atccgcctgc cccaggtcca gcgtcaggac cagccggcga
    36241 cctgtccggg gatccacgcc gtccgggtac ccggcctcgg cgagcaggcg ccgcgccctc
    36301 tcgagatcga agggaaacgg cccctcgccc tccggcgctc cggccacgcc cggcgggatg
    36361 ggcccgttgg gccgaacgat gcgcccgttg tagaaacgcg tccactcccc cgtgtcgaac
    36421 gcgcaggtca gcgcctggcg cagcagcctg ttcgtcccca ccacgggatc gtccatgttg
    36481 aagccgatgt aaaaaaggtc cagcgtcggc gcgcgcacca tccggatgcc ccgcgcggcc
    36541 agcgcaggct ccaggtcgcc accgcctctg acaacggcat cccagttgtc ccgcgacacg
    36601 gccgatgtct ccaggcgtcc atcgaggaac atgagccagg ccgtcgcggg atccccgatg
    36661 acgtactgga ccacgcggtc aagggccggg atcgggtgcc cctccgcgtc ccggtcgatc
    36721 cgtcccgccg cggcccagac cgggttgcgg gcgaactcga tccgatagtt cggacggtaa
    36781 tcggccagga cgaacggccc ggtgccaacg gggtgcctgt cgaattccgc tccgtagtac
    36841 tccaccgcct cgcgcgacac cgcggacgcg tagggcatgg tcagcaccca cagcagttga
    36901 ggatagggcc gcgtcaggcg gagttggagg gtatgcggat ccagggcaag cgctccctcg
    36961 acgcgctgcg agtaatccgt gggctcgctt cccgcggacg cctcgcggaa atcgtcgagc
    37021 ccggccaggc ggtcgcggaa gatccagtag ccggatgccg cggtcttgcg gtccgcgagg
    37081 cgcttgatgg aatagacgaa atcctccgcc gtgagctcgc gcccctttcc gcccgggaag
    37141 caggggtcgt cctggaaacg gattccccgg cgaaggcgga acgtgtagac gaggccgtcc
    37201 ggcgagacct ccggcagggc ctcggccagc agcggctcga cgcggtaggg ccggtcgcgg
    37261 taggcgtatt gcaggagccc ctcgtagatc cggctgacag cctgcgcgga ggcgacatcc
    37321 gccgcgcggg ccgggtccag cgttcggatg cgcgcggtct ggctgcgcca ggcgcgggcc
    37381 gccggggagg cgatccggtc tccgcacgcg ggcaggaaaa gaagcagaag caacgctgca
    37441 gggatgctcc cgaaacaagc caccctcccg ggccaggctc gggagggtgt tggggcccgc
    37501 gtcgtcacgc ggtggctctc tctattccgc ggccggggcc ggttggggtt cggctaccgg
    37561 ggccggagcc tccgccggca gcgcggcggc cggttccatg ggcgaagccg gctgcgccgc
    37621 gggcgcttcc tgcaggccca tgccggccgg ggcgtcggga ccggggcccg cgatcggccc
    37681 ggagggcgcg acgggggcgg ccggccgtcc catcatctgg ctgctcatca acgactgcgc
    37741 gcgatccccg gaataggtca gggccagcag cgtcgtcgtg accaggaata cagccgcgaa
    37801 gatgaccgtc agcttctgca ggatattccc cgcgcgcgat ccgaacaggc tctcgcccat
    37861 gcccgcgccg aaggccaacc ccagcccctc gctcttggag cgctggatga gaatcagccc
    37921 gatcagcgcc agactgcaga tgatttcaac cacgaccagc aaaatgcgca tcaacatatc
    37981 gaccccgctt tccgttcact tccttacagg gcgccccggc aaatggcaag gaaagacgcc
    38041 gcttccaggc tggcgccgcc gatcagcccg ccgtcgatgt ccggctggtg gaacagttca
    38101 gcggcgttgg acgccttgac gctcccgccg tactggatgc gcaccgactg cgcgatgtga
    38161 tcgtcggacc gttccttcaa gacgcgccgg atgaaggcgt gcacatcctg cgcctgcgcc
    38221 ggcgtcgcgg tcttgcccgt gccgatggcc cagaccggct cgtaggccac cacggtcttg
    38281 agcaggtcct gcccgctcaa gccggccaga ctgccgcgca cctgcgcgcc gaccacggcc
    38341 tccgtgcggc cggactcgcg ctcctccagg agttcgccga cgcacacgat cgggcggagc
    38401 cccgcggcca gcgccgcctt cgccttcttg ttgacgatct cgtccgtctc cttgaagtac
    38461 tgacgccgct cgctgtgccc caggatgacg tagtggcagc ccagctcgcg gatcatcggg
    38521 gccgcgacct cgcccgtgaa cgcgccggac ttctcccagt gcatgttctg ggcgcccagg
    38581 tccagcttcg agccggcgat cgcctgcccg acggcctgca gcgcggtgct gggcgggcac
    38641 aggacaacgt ccacggcgtc caggccctgc aggcctgcct tgatttccgc gacgagggcc
    38701 accgcctccg ccacggtctt gttcatcttc cagttaccgg ccacaatagg tttgcgcatg
    38761 ggaggttcca gggttcaggg ttcagggctg gccgcccaag cccccggggc atgggcaggt
    38821 tcagtcccac tattcactat tcactcttca cttgtccgtc aatgccacga cgccgggcag
    38881 ttgcttgcct tcgaggaact ccaggctcgc gccgccgccg gtgctgatgt ggctcatctt
    38941 gcccgccagg ccgctcttgt tcacggcgct gacgctgtcg ccgccgccga tgatgctcaa
    39001 gcacttcgtc tcggccacgg ccttcgcgat ggccagcgtg cccgcggcga acggggccat
    39061 ttcgaagcag cccatcgggc cgttccagac caccgtcttc gccttgcgga tctcggccgc
    39121 aaacagcgcc gcgctcttcg gcccgatgtc cagcgccatc cagccgtccg ggatcgactc
    39181 gccgaccgcc ctggtctgcg ccgcggcgtc gaacttgtcg gcgatgacgt tgtccacggg
    39241 caggagcagc ttgaccttcg cggccgccgc cttggcgagg atatccttcg ccaggtcgac
    39301 cttgtcctct tcgaccaggg atttgccggt cgcgaggccc ttggcgcggt agaacgtgta
    39361 cgccatggcc ccgccgatga ccagggcgtc caccttggtc agcaggttcg tgatgacgtt
    39421 gaccttgtcg ctgaccttgg cgccgccgag gatcgccacg aacgggcgct ccgggctggc
    39481 cagggctcgg cccatgaaat cgatttcctt ctccatcagc aagccggcga cgtttttctt
    39541 gtagaactgg cacacgatcg ccgtcgaggc gtgcgcgcgg tgcgcggagc cgaacgcgtc
    39601 gttgatgtag atctcgccgt ctccgagggc ggccagtttc ttcgcgaact ccttctgttt
    39661 cgccttcatc tcggccttcg cggccttctt ctgctcctcc ggggtgtcgt cgggcagctt
    39721 gaccttgccc tcctcctctt tgtagaagcg cgtgttgtcc accagcagcg cctcgcccgg
    39781 cttgagcgcc tgggcaaggg ccagcgcctc cgcgccgatg cagtcgccgc cgaactggac
    39841 cggccgcccg agcagctcct gcaggcgcgc cgcgaccggc ttcaggctga actccgcctc
    39901 gaaccccttc cccttcggcc ggccgaggtg gctcatcagg accagcgcgc cgccctggtc
    39961 gagcacgcgc ttgatcgtcg gcagcgcggc cgcgatgcgg gtgttgtcgt tgacaacgcc
    40021 gtccctgatc ggcacgttga aatccacccg gcacagcacg cgccggccct tgaagttcac
    40081 gtcgcggatg gtcagcttgt tcatggtctc tgtctccctg gagggcgatt ccgggtaggg
    40141 cgggctcgcc gagcctgccg tttcttccac ggcgctttcg gcgaaagcgc cctacccgtt
    40201 tggcctcgtt acttcgcgga cgccatgtag acgatgaagt ccaccagctt gttggagtag
    40261 ccccactcgt tgtcgtacca cgagacgacc ttgacgaagt tggagttcag catgatgccc
    40321 gcgcccgcgt cgaagatcga ggtgcgcgcg tcatggttga agtccgacga caccacctcg
    40381 tcctccgtgt agcccaggat gcccttgagt tcgccttcgg acgcggcctt catggcggcc
    40441 ttgatcgccg cataggccgt ctcggcgtcc gcgcccggcg ccttctggag gcggcaggtc
    40501 aggtccacga cggaaacgtt cagggtcggg atacggaacg ccatgccggt caacttgccc
    40561 ttgagggccg ggatgacctt gcccaccgcc ttggcggcgc ctgtggagga cgggatgata
    40621 ttgcccgccg ccgcgcgacc gccgcggaaa tccttcttgc cgcccgggcc gtcgacggtc
    40681 ttctgggtgg ccgtcgtcgc gtgcacggtc gtcatcaggc cttccatgat gccccagttg
    40741 tcgttcagta ccttggccac gggcgccaag caattggtcg tgcaggaggc gcaggacacg
    40801 aactgctcgc ccttgaagtt cttgtggttg acgcccatga cgaacatcgg cgtgtcgtcc
    40861 ttggacgggg cggacatgac gacgaacttc gcgccggcct tgatgtggcc ctcggccttg
    40921 gccttctcca ggaacaggcc cgtcgattcg cagatgtact cggcgccgac ttcgttccac
    40981 ttcaggttgg cgggatcctt ctccgcggtg aggcggatgc tcttcccgtt gacgatcagc
    41041 gcgccctcgc ctgccttgac cgtgcccttg aacgggccgt ggatcgtgtc gtacttcagc
    41101 atgtacgcca gctgcttggc gtcgaacagg tcgttgatgc cgacgacctc gactttcggg
    41161 ttctcgacag ccgcccggaa aacaagccgg ccgattcggc cgaatccatt gatgcccaac
    41221 ttgatagcca tttctgcgtc tccctttttt attttttgcg gccggccctg attagggacc
    41281 gggccgattg cccatggtta aacggagccg agaccataca ggatgggccc cgggccgtca
    41341 atcatgcact tttccttggc gtgtatcgct tttccgcggt cccggcttga cgctctgccg
    41401 tttcccgcta ggctgcaacc gatgaacgat atcccgccga ctccggacgc gcctgtggcg
    41461 ctgaccatcg ccggctccga cagcggcggc ggggcgggca tccaagcgga ccttctgacc
    41521 ttcgcggctt tcggcgtctt cggcacttcg gcgctgacct gcatcacggc ccagaacccg
    41581 cgcgaggtaa ccggcatcca ggcgctgcct cccgaattcg tcgcccggca gatcaagacg
    41641 gtctgcgacg ccttccccgt ggcggcggcc aagacgggca tgctgttctc gtcccccatc
    41701 atccgggccg tggcggccgc cgacgtccaa cagggcattc ccatcctggt cgtggatccg
    41761 gtgatggtgg cggcttcggg cgcccgcctg ctggaggcgg atgcgatcga ggccttgtgc
    41821 ggggaactgc tttcccaggc ccgcgtgatc acgcccaacc tggacgaggc ggccatcctg
    41881 tgcggccgcg ccatcgaaag cgtggagcag atgcgcgaag cggcgcgcga gatcggggac
    41941 cgctacgacg tggcgtgcgt catcaagggc ggccatctcg cggggccgga aatcaccgac
    42001 gtgctctacg acgaggggca ggaactggtc atggccggcg cccgcctcgc ccaggccgag
    42061 acacacgggg ccggttgcgc gttctcggcc gcgattaccg ccggcctggc aaagggctgg
    42121 ctgctggaag acgcggtgac ccgggcgcgc gaattcgtcc gccaagccat ggaatcggcc
    42181 cggccggccg gcgcgcatcg cccccttcat ttcctcgcga actacgaacc cgacgcatcc
    42241 gattgaatcg ccatggcggt tgagccgatg gaactggcgg cgggaacgga cggtcgcgca
    42301 tcgccccggc gcccgttcat gggcctggcc gcgttcttcc tggccggcgt aggcgccggg
    42361 ctgtgcctgg acgttccctg tcccctgctg ggcgccggcg ccgcgctgct ggccgtcgcc
    42421 ggcatgctgg cgcggcatgc cgcgggatcc ctgttgaccg gcctggcggt cctgctcgcc
    42481 ggctggctca atgtctcgct ggccgcccgg agtccgtccc cgcgcgaagt ccacgccctg
    42541 atggcgcgcg atcgcgaaca ggtggaactg gtcggcctgg tgaccggcga ctcggtcacc
    42601 accgcggccc gccgcgaggg tcaggtcatc tgccgcttct cgctgcgcgt ggaggggctt
    42661 caccggaaga cagactggga gcgggcgcgg ggaacggtat cgattttctg gcgcaaggat
    42721 gcggccggcc ccgaaccgcg ctatggcgaa cggtgggtct ggcgcggcgt gatcgagcgt
    42781 cgtccccgct tcgagagcgg aggcggcgga tccctctgcg ggatgactcc ggacccggac
    42841 ggcggccgga aactttccag cgggcacgga tggaagttcg tctcctggtg cctggcccaa
    42901 cggcgcgcct gctttgatct gctcggcctg ggcctggagc gaagcccgga cgtcgcggcc
    42961 ctgctgcgca ccctgctgct tggctaccgg caggagctgt ccccgcagct cctgcaggcc
    43021 ttctccacga ccggcacgct ccatattatt tccatctccg gtctccacgt ggggatcatg
    43081 gtcgtgctga tcacggccct gctgaaggcg ctcggccggc cccggcctcg ctgggcgctg
    43141 tacctggcgc cggcgctgct ggtctattcg atctccaccg gcttgagcgc cagcacggtc
    43201 cgggccacca tcatggccat gatcttctgg gccggcccgc tgttccgccg ccggccggac
    43261 ggcctctcgg cgctggccct ctccgccatc ctgatcctgg cgttcgcccc gttgcagctc
    43321 gtggacctgg gctttgtttt ttcgtattcc gccgtggccg ccctgatgat gtggtatccc
    43381 gtctgggtca ccccgttcgc ggcccggctg cgcgcggatc cctgggcgcc gccggaaagc
    43441 aaggggcggc gcatcctgcg ccagctcgcg ctgtatgtat tttccatgct gctgaccacg
    43501 gcggccgtat ggatcaccac gaccccgctt tcggcccgct tcttcaacct cgtcgcgccg
    43561 atggcgctgc ccagcaacct gctggtgatc ccgttgtcgt tcgtggtcct ggtgaccggc
    43621 gtgctgaccg tcctgttcgg cgccctgtcc tcgtggatgg ccgaggtgtt caaccatgcc
    43681 aaccgcgtgt tcacatccgt gatgctggag agcatcgagt ggttttcgag ctggcgcgga
    43741 ggctacacct acgtccccac cccgcccgcc tggttcgtgg cggcctggtt cgcctggatg
    43801 gcgctcctgc gcatggcgcg ccgccgcgcc gtcattgccg ccgccggact tgtcatgctc
    43861 ggcgccggct gggcctggat tctacgcggc gacgaggcct gcgcggacgt gctggatgtc
    43921 ggccaggggc aggcggtttt tctcaacctg ccgggctccc gagatgtcct ggtggacacg
    43981 gggccccgct tctcgtcccg ggacgtgatc cggcacctgc gccgccacgg cgtcaacggc
    44041 ctgcgcgccc tcgtcctgac ccacggggac gccgagcata tcggcgggac cgccgcggtc
    44101 ctggacgccc tgcccgtgga cgagatctgg tgcgcgccgc acctgggccg gtcgccggcc
    44161 taccgcgaat tgctggccga tatccgccgc cgcggcatcc cgctccgcgc gctgacgcgc
    44221 ggcgaccggg gccttcttcc cggcggcctg gagtgggaag ttctgcaccc gcccgccgac
    44281 cggaatttcc gccggtcgga cgaggctacc ctggtcctgc gcgtgggacg ggaggccacg
    44341 gcgctgatga tcatgggcgg ggcggacggc agcgtggagc aggacctggt ggcgcagccc
    44401 gcggacctcg gcgccacggt gctggtgacc ggcgaccacg gggcggaagg gacctgccgg
    44461 atcgactggc tggaacgggt gggtcccgtc gcggccgtaa tcagcgtggg cctcggcaac
    44521 gtgcgcggac aaccggacag ggcaacgctt tcgcgtctgc gggaaaaatc gattccgacc
    44581 tggaggacgg acgaaagcgg tcccctgcgc atccccttcc agcattcggc gcccggccgc
    44641 aagacgcccg ccgtccagcc gctggtcagt cctgcgcccc cagttccagg agcagttcct
    44701 ggaagcccgg atcgttccga acgagatcaa actccggacg ggacatccat gccagcgcct
    44761 gcttcgggtc caccaggctg atggcccgtt tgagcgcgtc gatggcgctt tccggactgt
    44821 ttccatggga gagggcctgg gcgagacgga aatggatcgg cgcgacatcc ggcgcgacgc
    44881 gggcgatctg ctgaagcagc ttggcggcgc ggtcccattg ctccaactga agcaggatca
    44941 gggcgtatgt ctgccctgcc atggtgtcgc ccggctggag ggtcatgtac tcctcgaagc
    45001 gctggctggc cttgtccggc tggccgcgcc gtaaatatag ggtggccagg ttgaaatagg
    45061 agttcgcgta ctgcgggttc atccgcgtgg cggtcaggaa ggacgcctcc gccttgtcat
    45121 aatcctccat gcccatgtag gctacgccga ggttgttggc cattgagaag gtcatgggct
    45181 cctcgcggga cgccttttca aaggcttccg cggccacggc gaactgcttg aggcgaatgg
    45241 ccagcagccc gacctggcct tgaacgcccc ggatgtcggg gaagatctgg gcgaccttct
    45301 ggtagctgtc gagggcgtcg tcgaaacgct cctgcttcgc cgcccgctcc gccttcacca
    45361 ggtaatacgc ggcctgcttg agggatgcgg acgtcaaggg caggtccttg agttcctccg
    45421 gggccgccgg gggcttcaat tcctccagca ggtacatcgt ctgcgcatcg gcctcgaaac
    45481 gcaggggcga ggccgggcgc tccagcgtcc ggcgaggtgt acgggccacc tgccggctct
    45541 cgctgatctg gaggtaatag taggtggtca ccacgacgat cagcaggccc gacaccagca
    45601 cggtcagcac gaacagcagc gtctgccggc ggagctggcg ctgggattcg gcccaatagc
    45661 cctccggcgc ctggggcggc ccggccgcgg gcggcgcccc ggcgggggcc ttgtcgcgga
    45721 cgtgatatac gctgtgggaa agcggatcca tgcctcactc cgtttccgcc gtctcgggcg
    45781 ctttttcgga cagggaggac gagaggaggg gctcgttgct gtaggcgtcc cacggcgcga
    45841 cggcctcgtc tccgcccgtc agttgccggg cacggtcctt cttttcgagc tcactctcga
    45901 aggcgcggcc ggcgtcggtt ttgcgcagct cggcgaaggc aggaccccgg aagctctgca
    45961 gcacgacgga gggcggcgcc ttggccgccg cgtgctccag atgcgcgcgg gccttgtcca
    46021 tgtcgcccat cagcgccgcc acggccgccg catccatgat gagcgggatc cagtcgggct
    46081 tcttctgcag cgccccggcc tggatcgcgt aggcctccgc gtagcggctc ctcgacgcct
    46141 cgaggaaggc ctgttccttg agcgccttgg gatcgccggg cgcctgggcc aggtatcgct
    46201 ccaactccgc ggccgccgcg gcggcatcgg actggagcag gaagcagagc gcccggttga
    46261 agtgggcggg ggcaaactgc gggtcgtact gcaacgccgc ctcgaggagt tccgccgcct
    46321 tggcggcgcg attctggtag aaataggcca ccgccaggtc gttcagcagg gccggggaat
    46381 ccggctcggt gccgatcgcc gactgcagga cgatcagggc cttgttgtac tcgcgttgac
    46441 ggaggttcag gcggccgagc tcgccgcgcg ccgagttcag gtccggccac aggcgcagcg
    46501 cctcggagta catttccgcg gcctggacgt actgcccggc ggactgcagc aggtcgccac
    46561 gccgggccag cagcgtggcc tcgttcgcga gctcggcgtc cgagcgacgg accccggaaa
    46621 gcaactcctg cgtctccacg cgcttccggt cgagatcgac cggcgcgccg gtcaccgagg
    46681 cgctgtccgg gatgtcccgg gtccgcgcgt tctctttcat ttgccaggtc tttcgggaga
    46741 tgaagaggac gatgagcagg aacatgccca tgctcgccag caccaggagg acggtatacc
    46801 acgcggcgtg ggatgtcgcg cggttatcgc ggcgaggcgc cggacggctg tcccggcccc
    46861 gcctcgacgg gtcgttctgc ctctggcgat ggtattggta cccggccgcg ccgccggttc
    46921 cgcccggctc ggcatccggc ggaggagatg agatcggatt catgattgca gttttggctg
    46981 ctgactttag tacatgggcg cgcattgtgc aagccatgcc gcgccgggcc ggctgttgca
    47041 ttgccggcgg gggtgcgcta taccttgcct gcagcccggc ccgcgggatt cctctccggc
    47101 cgggcgggag acgggtgcca tgtccttgag aacattaggt cgcggagtcg gctggctggc
    47161 gctggcggtc ctgctgaccg ccaacctggt cctgggcgcg cgcctgcaga accaggccgg
    47221 ccctcccgat gagcgggaca ccggctacga aaagatggcg ctgttcaccc gcgttctgga
    47281 gcaggtgcgc gagcaatacg tcgacgcgga caagaccacc taccaggacc tgatctacgg
    47341 cgcgctgcgc ggaatgctgg aatccctgga cccgcacagc cagttcctcg atcccgacat
    47401 gtacaaggat atgaaggacg aaaccgccgg cgagttcggc ggcctcggca tcgtgatcgg
    47461 ggtcaaggag ggcatgctga cggtcatcgc cccgatggag gacacgcccg gcttcaaggc
    47521 cggactgctc ccgggcgaca agatcctgga aatcgacggc aagcccaccg acaacctgac
    47581 catggaggaa gccgtcaagc aactgcgcgg cgccccggga accaaggtga cgctgggcat
    47641 cctccgcccg aaaagcgagg gcatacgcac gatggagctc gtgcgggccg tcatcaacat
    47701 cccgagcgtc aaggacacgg acatgctgga ggacggcatc ggctacctcc gcatcgtgca
    47761 gttcaacgag ccgacggcga aggccgtgca cgaggcgatc caggggctcc aggcccgggg
    47821 catgcgcgcg ctggtcctcg acctccggaa caatccgggc ggcctgctga cctcggcgat
    47881 cgaggtcgcg gagaaattcg tccggcgcgg cgacatgatc gttttcaccc agggccgcaa
    47941 cgaaaaaccg ctccagacgt tccgcgcgaa aggccgccag cattacaccg acttcccgat
    48001 ggtcatcctc atcaacggcg gcagcgccag cgcggccgag atcgtggccg gcgcgctcca
    48061 ggaccaccgc cgcgccatcc tcgtgggcgg gaaatcgttc ggcaagggct ccgtgcagag
    48121 cgtcctccag ctcgacgacg ggtccgccat ccggctgaca accgcgcgtt actacacgcc
    48181 gagcaagcgg gtcatccacg agcagggcat cgaaccggat gtccccgtcg ccatgccccc
    48241 ggaagacctg cagcggctga tcaaccagcg cgccgagccc gaaagcggga aggacgaagc
    48301 cgatgttccc gcgagcgcga tccgcgacgt gcagctcgag cgggccctgg acatcctgaa
    48361 gggcgtgatg atcttcaccg cgcagcagcg cacctccgcc gtcgcccggc gttgaagggg
    48421 gcgggttcag ggtccagggc gggttccggc gttctcccat gaatgttctc ggcatcgaaa
    48481 cctcgtgcga cgagacggcg gccgccgtgg tggcggacgg ctggaaggtg ctctccaatt
    48541 gcatctacag ccaggtggcc agccatcggc cctacggcgg cgtggtcccg gaaatcgcct
    48601 cgcgccagca cgtggaaatc ctgcccggga tccttcgcga agcgatcggg cagtccggcc
    48661 tcggctggga cgacctggat gccttcgccg tgacccgcgg cccgggcctg gccagctcgc
    48721 tgctcgtggg cttgtccgcg gcccagtcgc tggccttgcg ccagcggcgc ccgctcatcc
    48781 cggtcaacca tgtggaagcg cacctctact cgctgtttct tcccgagccc ccggctgcgg
    48841 ctcccccgcc cgccccgccc ctgcccatgc tggtcctgat ggtctccggc ggccacacgg
    48901 cgctggtccg attcgagggc ctcgggcgat accggtggct gggccagagc ctggacgacg
    48961 ccgcgggcga agccctggac aagggcgcca acctgatggg cctgggctat cccggcgggc
    49021 cggccatcga gcaggccgcc gcgggaggcg atccggaggc gatctccttt tcccgcacgt
    49081 ccatccgggc cagcccgcgc accggcgacc tcgatcccga cctgtgtttc agcttcagcg
    49141 gcctgaagac ggcccttctc tatctcctgc gccggcaacc cgaactcctc ggcccggacc
    49201 gcctccggca cctcgccgcc agcttccagg aagccgtgtt caacacgctg ctccaccggg
    49261 cggaacaggc catggaaaaa gacgcctatg cctcgttcgg ctgtgtcggc ggcgtgctgc
    49321 gcaatcgccg gctgcgcgcg cggatcgagg aggccgcccg gcggcgacgc ctcccgctgc
    49381 gcatggcgcc cccctccttc tgcaccgata acgccgcgat ggtcgccggg ctggccggcg
    49441 cgacgggggg accggcgccc gccgatccgg ccgcagtgga aatccggccc gacctgcgcc
    49501 tgtcggcctg accgcaacgc ccgccggaaa gccgggacgg cctacaatgc ctttgacgct
    49561 gccgcccggc ttctttatgc tgcccgccaa tagatcgcca tgactgcgtc ctccagcccc
    49621 gccgccgcgt ttttcaaatc gctcgtgctg tcgctcgacc tcccggtcat cgtcatggac
    49681 gccgaactcc gcgtgctgct gctcaacccg gcggccgagg ggatcacggg cgcgcgcacc
    49741 cgccaggccg tcggccaatt cggcggcgag ctggtcggca tggaccgggc cacctgcgag
    49801 gccctcgggt tcggctcggg cggcggcgcc acgggctgga aggagttcgt ctgcgttccc
    49861 gcgggttccg ccagccgcct ggtgcgggcc cggggctgca ccctgccctg gagcgcccgg
    49921 cgcaccgcgg tggtctggat tctcgacgcc cccggctggc acgacgcggg cccccgcctc
    49981 ctgccgtccg gcgcccccgt catcatcttc gagctcgacg ccggcggccg cctcgtctac
    50041 ggcgcggagg agttcgccgg cctgctcgac cgccaggccg acgtcacgac cgggcgcctc
    50101 ttccgcgaac tgctcgtgga ggagcagcgg ggttcgttcg acgccgtctg gcgcaaggcg
    50161 cacggcgggg aggcggtccg caactcggag ttgcgcctgg tgcacggccg cggagcggcg
    50221 cggcctttct gggtttcgct gttcccgatc aaggatccgc gcggcatcac cctcggggtc
    50281 cgcggcgtgg ccggcgacct ctccgggcag aaggacctgg cctatgccct cgaggcctcg
    50341 gaagagcgct tcagcgtcct gttccgcgaa tcctcggacc ccatcctgat cctcaacatg
    50401 gagggcgaca tcctcctggc caacccgtcc ttcgaacgga tcacgggggt ccgctccgac
    50461 gagctcttca gcggcgaaaa aagctggtcc gatttcgtcc tgcccgccga ttacgaagcg
    50521 ctccgcggcg aactccggca atgcgccgcg acgcacaagg acggcatgac cgagttccgg
    50581 gcccgggatg ccggcgggca ggtggtctgg ttcgagcaga gccacagcat cctgcacgac
    50641 gaagtgggcc agccccgcgg gatcatggcg gtcgcgcgca acatccaccg ccacaagcag
    50701 cacgaattcg agctgcgcga gcaggcggag gacatgcagc ggcggcacga ccgcgcccag
    50761 gccctgatct cgcgcctgaa attgttcttc gcccagacca gctcgctggc cgacgatccg
    50821 cagcggttcc tccagggcgt gtgcgacatc ctgttcgaga tgtacgagcc cctgctcgtg
    50881 atccttcacc tgtccggcga aggccgggtg gtccaccgcg gccggccgga tgtgctggcc
    50941 gatgtcgggc cggaagccct ggagcaactg caatgcggcg tgtgcgggca gatcaccgaa
    51001 acgggcatgc cgttctactc ccactcgctc gacacggccc cgccgccgca atgcgccgcc
    51061 cggctgggtt cgcggtcgct gaaaacctgc gtcggagcgc acctccgcga ctcgagcgga
    51121 aggatccgcg gctccatcat catgctggac gacaagaccc gcgccttcga cagcctcgac
    51181 gtcgaggtgg tcaccgtcgc cgccctgcag gtcgccgccc ggctccgcgc cgaggagcag
    51241 gagaccgtgc gccggggcct cgaggagcac ctgcgccagg cccagaagat ggaagccgtc
    51301 ggcatgctgg ccggcggcat cgcccacgac ttcaacaaca tcttgagcag catcctcggc
    51361 ttcacgacct atctcctggc ccggacgccg cccggcagca acatccaccg cgacctcggc
    51421 ctgatcgagc aatcggcggt gcgagccgcg gacctgacgc gccagctgct ctccttcgcg
    51481 cggcggcggc acttcgcgaa ggagcccgtg tccttcaaca cggtcatcca cgacgtcgtg
    51541 gccatcctcc gccgatcgct gccggccaac atccagatcc atatgcggct ggccccggaa
    51601 ctgccccccg tgctcggcga ccgcgggcag ctcaaccagg tgatcatgaa catgggcatc
    51661 aatgccgccg aggccatgag cggcaaggtg ggcatgctca cgatgccgta ctactttttc
    51721 gcggaaatgc tcag
//
LOCUS       DAOOTK010000010        50030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_536_length_50030_cov_5.510315, whole genome shotgun sequence.
ACCESSION   DAOOTK010000010 DAOOTK010000000
VERSION     DAOOTK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 50030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50030
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_50030_cov_5.510315"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK634_02405"
     CDS             <1..459
                     /locus_tag="PK634_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96620.1"
                     /translation="RVSRFRVGARGGSVTLSKSNFLITLAPYFFPLYTILVIALHGLL
                     ALFYDLRAYEPLWLGFVGLTWSFHLTFTVTTLRQRQPDIRIHGRLFSYALIYLLNLLG
                     VGLGIVAVAAPTLAEFGAQLGADMLNLWELGVRLSRGAQAWLQAHGPARP"
     gene            complement(471..908)
                     /locus_tag="PK634_02410"
     CDS             complement(471..908)
                     /locus_tag="PK634_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOW96621.1"
                     /translation="MPAVSEMAGQVSELRVGPNDPVRRGDIVAVLAPARDEVEESRQA
                     LASAETTLAAARTEYLKQVQLRETGRPASPGDEEAARVAMEEASEDRNEALRRIQQVQ
                     REDNRRWIRSPAGGRVTAILVQSNQAVSAGQVVMMMEPGGPPP"
     gene            1234..7347
                     /locus_tag="PK634_02415"
     CDS             1234..7347
                     /locus_tag="PK634_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOW96622.1"
                     /translation="MAFGVLALPALASVGNVTGIARNGDTLLISAGVDTVVVQACRAN
                     LLLVDYRPSGRTSANTLMIATTNWPAAGATINTNGDPILIDTTNLHVEIARAPLRIAV
                     YDATGTNLLIREQAAEGLFADGVRFHHRPGSDFYGIGGYNAWDDSSAGMLRNSGGWVE
                     AGYQGDAGAPLAWTRQGFGVLVDSDGGQFTIGGTNTVFEYCSKTNILLYIAVGPPADI
                     LAAAAEVTGKAPLFPKYAMGFANTEWDITQGELTNIVNGYRSRAIPIDHYIVDFDWKA
                     WSEDNYGEWRWNTTKFPGGPSGALHSQMRAAGMHLSGIMKPRIFVDTVQGNYATANGF
                     WWPGSSEYQDYFTGGWVNDLNFALSACRDWYWDHITNAFSTGITGWWNDEADQKGGGG
                     GFFDNWEFMNMQRSLYEGQRGYSTQRVWSINRNFYLGAQRYAYAMWSGDIDTGFSAMA
                     AQRERMLSALNLGQAWWGMDIGGFNGGDPSSENYARWIQFGAFVPVFRVHGQQYAQRQ
                     PWIYGSTAETAAVKAIRLRYQLLPYIYSCAGRVTETGAGFVRPLFHDFPTDANAANYK
                     DAWMFGDALLAAPVVAEGQTAKSIYLPAGTWFDYFRGTRYAGGQTLSYSLNSTTWTDI
                     PLFVRQGAILPSQPAMNYAGEFPLTNVSVDIFPDTATSTFAYYDDDGTSYAYETGRLF
                     RQEFTVQDRGESMAVDISAASGSYVPPLQTYFCRLYCPTASAVTINGAAATPYASVAA
                     LEAAAGEGWALGTNRFGPLVAVKVAAGAAKSIVASNNAVATPAFAPPGGTFTGAVLVA
                     ISCATEGASVYTTTDGSEPDETDALYAEPLLLAVSTTVKARGFKAGRTPSVVAEASFA
                     RDDNLLRNGGFEAQGSSTNNALYWTTGEPDEHGETWGSGLRVSWRSHSDTWQGAIRGT
                     WAGAGSEGGFWQEAPAVPGRAYRFSAWLWADSDWAPSLQALKIEFFSGATMGETMLVA
                     HTNTFTGVGPTWTNRAVQATAPTNADWVRVVVVASGVSGSGALQFDDLRLDPTNVCTL
                     TIESPHGSPSPAIGAHLVDQGRTITNSVNSPVTSGETQFVCAGWTLAEHAPTSGAGAV
                     MTMTVTNNAVLTWRWTTNLLNPSYLSFSSLAYTSAEADVTARLAIVRSGGSNGEVSVS
                     YRTTGGSASAGLDYAAVTGTVVLADGVVTGSAAVELFHDLQFESGETVEVSLFNPSGA
                     TLAAPTGAVLTILDDDADFGTRTLSVISTHGQPDPPAGLQSFDYGIRLTASVTGTAEQ
                     ATTQYLCIGWAGTESVPASGTTNTTPSFLLTNDSSIAWQWTTNVYFSPAAGPNGSVGG
                     DASGWYPLGAGVVVTATPVTAYSFAGWTGDVPAGHELDNPLALTLGQARAVTATFSAD
                     AGENLLNNPGFEQAGTSSVRALYWDQGDPDTHGEMWGTAARVDWRSHGGTWEGAIQGQ
                     YSGAGTEGGFWQEVVASPGEEYRLSGWFWADDGNPYGPWGAGEQVIKLEFIENGVLLS
                     AATGTLAGVGQAWEERSVSALAPTNADWVRAVILARDISYDGSLQLDDMMLEMVPTLD
                     SPVIQHVVPVDGTSCVLEWIGVAGADGYIVDVATNGAFQPESRASDLFISEYGEGTGS
                     ERYIEIWNGTGAEVDLTAYRLWGISAGGSWFESSLVLSNTLAEGAAHVVVNDNCTSAV
                     LRAAADQFAANAAPMNFSGDDAIGLAKVIGVVTSLIDAVGTTGGDPGTGWGVAGVADA
                     TANHGLIRLASITAGNTDWSTCTNEWAVLPVNSWTNIGQHKMDVTPSDFVPGYEERPT
                     PGVTSLTVTGLATGVTYYFRVRATNAESFSVWSETFEAALASGYGILATAGEHGTVSP
                     SGLVMVAAGAGANFVVQAGPYYVIATILTNGAALTLPPGLTVYTAEWSQVFATGTFHS
                     TFAERQADAHATPWWWLATHGLTNEETSFNEAELGDTDTDGHAAWSEFVADTDPGDSN
                     SVLMAHPGGAAPSGAYTIRWPGSTGRYYHVYRGTNLALPFDRVAGNVPGIAPVTVHTD
                     AVFQGQSPAYYRVRALLAPEE"
     gene            7408..7482
                     /locus_tag="PK634_02420"
     tRNA            7408..7482
                     /locus_tag="PK634_02420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7440..7442,aa:Asn,seq:gtt)
     gene            7603..8025
                     /locus_tag="PK634_02425"
     CDS             7603..8025
                     /locus_tag="PK634_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96623.1"
                     /translation="MSIVRCLVVLAAGLALVAGCSKSEQEPAAPAAAPASEQAPAVAE
                     PAPAPAPAPMIEEVEQKAEEVTETAADVAAQAEASVKEAAGEAMEAASAAVETAKDTA
                     QDAAAAAGQAVEEIKADVMPAATETPPVPAPAEPQPQP"
     gene            complement(8092..9741)
                     /locus_tag="PK634_02430"
     CDS             complement(8092..9741)
                     /locus_tag="PK634_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOW96624.1"
                     /translation="MNRRDPFWAVAVFSLSYTVIAALVTTRTSNAEFMLYIGVMAVLM
                     ALIGLLHRCVRLTAGALWGLSGWGLLHMAGGLVPVPESWPIAGEIRVLYSWWIVPGRL
                     KYDMVVHACGFGVTTWVCWQGLRAALRNRGVAEPRPTAGLLILCVAAGMGFGALNEIV
                     EFAATLTVPETNVGGYVNTGWDLVSNLVGAVASASLIRAFAPRPGPARAHSPGALLAL
                     LMLAAAAHAGDVPAPAVRPVVNREYLPVLLDLIERATNSIEFLALEYHDDATVLRVEA
                     ALAAAVARGVRVQALVENKIEFNAAGVERLRALGAEAELDTPKKMLHSKLVVVDGRKV
                     LLGSTNLTGNSMDNNNETNLLLDDPVLAGAFQRYIAALRADSFAEPDVPSVESGGVKT
                     IVNRQYFPEVLSLFDSATRTVRVIMYGIKYEPKYKDSKVNRLVESLSTAHRRGVDVAV
                     IMDLSDYNDTLNKVNDAARQYLAASGVAVHDDAENITTHAKVIVADDRVVIGSVNWGY
                     DALERRNETCVVVRDPALAAYFAAYFDQIKGPSVDVAPREN"
     gene            9862..10830
                     /locus_tag="PK634_02435"
     CDS             9862..10830
                     /locus_tag="PK634_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOW96625.1"
                     /translation="MPRWKLARFVLALAAPAAFAQVPLDAGLPVYQPVEGISGTIKSV
                     GSDTLNNLMTLWAEGFRRMYPNVQVEIEGKGSSTAPPALIEGTATFGPMSRPMKEAEV
                     DGFKQKYGYAPLALKTSIDMLAVYVNKDNPVEGLTFAQVDAIFSRDRKLGHPEDLVNW
                     GQFGLPGEWATRPISLYGRNAASGTYGFFKEHVLGKGDYKDSVKEQPGSSAVVQGVAS
                     DRGGIGYSGIGYKTADVRAVPLAMAEGEDFVPATPDQAYTGDYPLARFLLVYVNRRPG
                     AELDPLQREFIRYVFSRQGQEDVLKDGYFPVTAAMAREALQAAGIE"
     gene            10851..13283
                     /locus_tag="PK634_02440"
     CDS             10851..13283
                     /locus_tag="PK634_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOW96626.1"
                     /translation="MQPARNRKTRWAVRASDSVSRGLITVGGIGTIVAVFTVCLFLVA
                     VALPLFRPPSIRGAATRSAPARLPPLLIQPDEDGRRAWALAADGSLSAFQMNDSSAAV
                     TERVFSNAPITAVSTSPDGANLALGFEDGTVRRGRVGWAEGAARADFGEAAAFSPPSP
                     VRLLAHAGESFCALTADGRLFFEKITERRNLVTGEVTPRRRAAVLPYEPRTNGEPSFL
                     LLSGLADWALLAWPDGLAIRYNTRQPEKAFIEETLNLLDDPALRLTAMRCLPGQTTVL
                     SGDSGGRVRAWFPVRPDGTNALTRLVAAREFRGAGAPVACLATAGRMRVVAIGYADGS
                     VRVGHVTSGKTLARLPAGPRGPAAALAFLPRDNGLLAIRPDGLSLWRLDPRHPEATWK
                     ALFAPIWYEGAPRPAHTWQSSAATDDFEPKLGLVPLIFGTLKATLYALLFGVPVALLA
                     AVFTSEFLHPRARRVVKPAIEMMASLPSVVLGFFGAIVIAPFVETAVPAVLVSLVTVP
                     VMFLAGAHVWQLLPPALTLRLASWRLLLLLLLLPLGLAAAAGLGPVAERLLFAGDMRR
                     WLDGQLGGPAGGWFFLMLAPAALAAALIMARAIRPRLQGAARSWPRSRVAAADALAFL
                     ADLALTGGLAWASAGLLTAAGWDPRGAALGTYVQRNALIVGFVMGFAVIPVVYTIADD
                     ALAAVPDHLRSAALGAGATPWQTAVTVIIPTAMSGLFSAVMIGLGRAVGETMIVLMAA
                     GNTPIMDWNPFNGFRTLSANIAVELPEAVRNSTHYRVLFLSALVLFALTFVINTAAEL
                     VRLRFRRKAVAI"
     gene            13280..14938
                     /gene="pstA"
                     /locus_tag="PK634_02445"
     CDS             13280..14938
                     /gene="pstA"
                     /locus_tag="PK634_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOW96627.1"
                     /translation="MSGGRAAPRRERTALFAQGEPMIWLTGGGLALALAMIAGLLALV
                     VVQGSVTLWPGPVTRVELNDGRAVMGEVARSESVGATGGDGPVRRRLFRTGNFELTGE
                     HFQWVADSDIRAESRPEWALVVERTAWGRFYGAPQAFLLDGRAAASDPASAWAMYEQH
                     RLAVQRRLRERQRLESRDIGRVNRRIESARLAVKSAELRHGMGSVPHREAVARFEAVE
                     RQGLAEHARLRGRIEALNRENARFQLALATADGWEQAIPLADIVRACAPNRLGLAAKW
                     AVYGSRWLEFLSGAPREANSEGGVFPALFGTALMTVVMSVAVVPFGVMAALYLREYAK
                     AGPLVSLVRIAINNLAGVPSIVFGVFGLGFFCYIVGGWLDRSFFAANLPNPTFGKGGL
                     LWSALTLALLTLPVVIVATEESLAAVPNSMREGSYACGAGKWQTIRRIVLPRALPGIL
                     TGTILAMARGAGEVAPLMLVGAVKLAPELPVDAVAPFLHPERSFMHLGFHIFDLGFQS
                     PNSEAARPMVFTVTLLLIALIFLLNLVAVLLRARLKKKFVTGAF"
     gene            14941..15822
                     /gene="pstB"
                     /locus_tag="PK634_02450"
     CDS             14941..15822
                     /gene="pstB"
                     /locus_tag="PK634_02450"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOW96628.1"
                     /translation="MNTLTATDTLTLEPPAPAGDDRLAAIARGGQFDAVVHPAVDRED
                     ATLEVRDFCLWYGPKKALHHIGMRIPRGKVTALIGPSGCGKSTLLRSVNRINDLLDTV
                     RIEGDMLLNGDSIYAPGVDVIELRKRMGMVFQKPNPFPMSVYENVVYPRRIDGERSRA
                     ALAEVCERSLRRAALWDEVKDRLQESAMSLSGGQQQRLCIARAIAAEPEVLLMDEPCS
                     ALDPIATNKIENLIDELRGRYTILIVTHNMQQASRTSDYTAFMYLGHLVEYGPTADLF
                     RKPRLRETEDYITGRFG"
     gene            15878..16585
                     /gene="phoU"
                     /locus_tag="PK634_02455"
     CDS             15878..16585
                     /gene="phoU"
                     /locus_tag="PK634_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOW96629.1"
                     /translation="MMIHLHKEIENLKKRLLSLCAVVEDNLRQSVQAITERDDRRAEQ
                     VIGRDLEIDQAEIEVEEECLKILALYQPVAIDLRFLVAVLKMNNDLERIGDLAVNIAQ
                     RAIFLNRELPIEVPFDLNGLSEKTQTMFRLSLDALVNLDASLARKVCAMDDEVDAINR
                     AMYDGVKAMIRRQPEHLDALIALLSISRYLERIADHATNIAEDVLYTVQGAIVRHARE
                     FKRLPPEDAPPASVRRD"
     gene            16696..21243
                     /locus_tag="PK634_02460"
     CDS             16696..21243
                     /locus_tag="PK634_02460"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96630.1"
                     /translation="MILRLASLFFVPLAPAATRFLPVPGPPLTHLRTDYQRPFLYALQ
                     EGATSTDACRLLFIDTRTDAIATNCPAGLNASDLAIVYGDTRLYVANWKQAACCTFDL
                     DAQASAGPVALPSECRRLSGGSQGRLLAEKEGVTTSSDVLLLESTSGTTRASWSGRLG
                     DGDIEPTRTNYFHAQNRQLFRLVVTTNAITSDGASEGFPEVGGWDDTVVLSADGQTIF
                     CQGRAIDRRLEVVRTFPEPIRAVSLHGEVAVGTQQVFSTATGDPLLALPAPSSVSAFS
                     GDQRKLYVYDSVSNGLYVVDLTNEIALSNAWLQPEPADGALMGRMPPLCWTPTPVADG
                     YDVYGGAISQEVALAGTGSVTFLGRTTAGRLTPTSALPVQSEYFWRVDALTSSGAVQG
                     AVWRFTSGPVVVQPGEINALTLEHAPTLATQLQVRAVATNVWALTAQAPWLSCTPTSG
                     LGASLVSVALRPEGLSPGRHESALRVESGAFTDTVPVVLQVLTQEVVRVAADRKRARV
                     YALHAAADQLSAVLAFDSASGVMTGLLFVGRGASDMELDPASDDLWCVSGFDSSLSRI
                     ALSTFDHVDTIPFNLGLSGAQVSIYQVAPGPSNYVYVADPYYLPAVHILEKGSGQEFT
                     GATANRTGDLTVNGDGASMLAWRYVSGTPAVWRLDCRTTNLTVVDTFDGHISGNPMDY
                     PTLVSPDGAFVIKHYTKLAYSNLASGAVRIFSEALWSWSPDGRLLAAGSNVLEEASGQ
                     RVYDLPFYTGLQVFSGDGSALVRIHPTTRQWSMVSAALYSAPPAPPAGPIPTDNAVVN
                     PAPDTLHWTVAERTVRYHVYLDTDAATVGAAEPGGTAYLGATTNAEWSLTHALACATT
                     YYWRVDSVGVYTTTTGTVWTFHVPAGTLSPILISTNIPEGFSPAPGIFDVALCGPALG
                     WTVACDCAWMQMAPTSGAGNDTVTGTYDTATLSPGVYTGRISLAMGRDVLAVPLSLTV
                     TTNPALHPIPGGVLFPSWVQVFDGESHADDRGAAAAMDPLDNVVVAGEVRGSGDGDDM
                     LLLKLDPNGQTLWATNYDGSGHGDDAVKDLGADGLGEIYALLDSTGTNGNMDLVVCKY
                     SADGQFLWEYRRNGTANTHDYATALAVPGTGGVVAAGMTVDAATSNDVFLARLNADGT
                     PAWVTTYNGPHETSRDECNDMSTDAGGSIYLTGTSTYPGPPQQWRVFKYSAGGTQEWS
                     QFWYAQSGAAIAVNEATNVWVVSGGGLLRNLDPATGVPRWERTLTFPYMTGFAAYALL
                     PGPRTQVYVAGRASDGKYRYAAAWVGSTGEVLATATVDRDEHSAGHKYLNTAILDAFW
                     QPYLTGKGGGTLRLTPSLGELGRVTGSPTEGIALAADSTGAVYMVGSSDNFTNQNDLV
                     VVKLAPSPSGDFDADQIPNDWEIKTGGNAYDAVPGQDPDDDSMTTWEEYLADTDPTNG
                     TSALAAPALVTRPDGFLYRITGTSTGRLYDIFWKTNLLDAATPWQFSGYSRLGTGGVL
                     EIVTNMPTVDVLFLRSGARLP"
     gene            21475..22572
                     /gene="ugpC"
                     /locus_tag="PK634_02465"
     CDS             21475..22572
                     /gene="ugpC"
                     /locus_tag="PK634_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOW96631.1"
                     /translation="MAKVILEHVCKTYPGGVTAVNDANLEIEDQEFVVLVGPSGCGKS
                     TTLRMVAGLEEISKGAIYIDGRKVNDVPPKDRDIAMVFQNYALYPHMTVYKNMAFGLK
                     LRRYPRDEIDRRVREAADILGIQDLLERKPKALSGGQRQRVAVGRAIVRKPKAFLFDE
                     PLSNLDAKMRVQMRAEISKLHTRLASTMIYVTHDQVEAMTMGDRIVVMKDGLIQQVAT
                     PLELYNRPANQFVAGFIGSPPMNFFPGTLEARAGGLFFQEGSFSVRTTDEQASALSAR
                     AGQPVVFGIRPEDVAEATGAVPAGERIAARVEVVEPMGAEVYVYLSTGKHSFVARVGP
                     QSAVEVNQTVPLTLNMKQAHFFHPETGDALA"
     gene            22614..23789
                     /locus_tag="PK634_02470"
     CDS             22614..23789
                     /locus_tag="PK634_02470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOW96632.1"
                     /translation="MEILTGIAQFLLVTAIAFGAFVLIASHMRFQRLTRAAAEPDAPD
                     APAADPLTAEVAHRLGTAHREPEPFAVILFEPAAAAGQALPDDLEPLLPPLEATLRGS
                     IRAGDGVLRHGGRQIGALLWITRDRVESVAQRLLERIRRPGEPGGRSLPLGIHLATGL
                     ATHPENGSRTHALLQAAHAALDSARAAGGWAARLAASDLPAVPPPAPAVPPKGILDEL
                     TGVLKEDRLITALQRSLSRHRKEDRPLSLLHLDLDHLDRYNEHYGRETGDTLLKGMAA
                     VLQKHAREADLIGRLEGDSFLLIMDCPPSRAVPATQRLLGELRKTPFAVPGGTLRVSA
                     SVGVAGYPDHAAHPRDLARAARYALRAAKRRGGGRPVLFEPAMQTIAEENPREVDSL"
     gene            23786..24544
                     /locus_tag="PK634_02475"
     CDS             23786..24544
                     /locus_tag="PK634_02475"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOW96633.1"
                     /translation="MSAAVPFRPLRLGVNLDHVATLRQVRGTEYPDVLDAARLCLGAG
                     AHGITVHLREDRRHIQDADVFRLKAELDAPLNLEMANAPGIVQVALQVRPAEVCLVPE
                     RRQELTTEGGLDVARQETALRPTIEALSDAGIEVSLFIEADARQLEAAARLGAPVVEL
                     HTGAYCDAAGEREAAAELKRLIEGARRAHALGLQVNAGHGLNTDNLPPVLRVPHLDTL
                     NIGHSLVARSVFVGLDSAVREMLDAMALYEGGEP"
     gene            24541..24975
                     /gene="acpS"
                     /locus_tag="PK634_02480"
     CDS             24541..24975
                     /gene="acpS"
                     /locus_tag="PK634_02480"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOW96634.1"
                     /translation="MRRTTARAGAPVLGVGVDLVENERMQSALRRWGRTFREKVFRPA
                     EQSYCDGKAEPWRHYAGRFAIKEAVSKAFGTGIGEHLGWLDIEVRRDPASGAPSVQLS
                     AKGGRLARRRGVGRVLVSLSHTRHYAVAQALLVGGARKEKTR"
     gene            24972..26543
                     /locus_tag="PK634_02485"
     CDS             24972..26543
                     /locus_tag="PK634_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOW96635.1"
                     /translation="MKIVTAEQMRELDRRTIEEAETPGEVLMERAGQGVADAVARLAE
                     TAGFHHPLVHLIAGRGNNGGDAFAAARFLKEDGFAVQVWIAGAANEIQGDALRHFSRM
                     KDAKITARELPTREDWEESLAYPAAGDLLVDGVLGTGGSGPARGPAAGAIEYINQQSR
                     QALVVAIDLPSGLNADTGAAEGAAVRADLTVTMGLPKRGLLEPAALEYVGALEVVDIG
                     IPAEYITQAALAEDREFIHPNDLRPLLPRRPRATHKGSQGHVLLIGGARGYAGAIAMA
                     ARAALRSGVGLVTVLTPAGVAPIVAGSVLEAMVQGGPETDTGSLSAAFWADWRNRIDK
                     FGAVLIGPGLTRNPEGTAIMRHLIRECPPPLVMDADALSLLEGQPHFIAKCRRTALIT
                     PHPGELARLMAQDIETIQQDRHAAARAAAESTGAVVVLKGAGTLVAETGKPVQMNLTG
                     NPGMATGGTGDVLAGLAAGLLAQGLTPYDAARLAVYLHGRAGDTAALHKSQAGLTALD
                     VLDELPYAFREISIR"
     gene            26612..26968
                     /locus_tag="PK634_02490"
     CDS             26612..26968
                     /locus_tag="PK634_02490"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOW96636.1"
                     /translation="MQRTMLKSKIHRATLTGADLHYEGSIAMDRRLLEAADILPGEQV
                     HVLNLNNGARLVTYAIAAPRGSGRMLLNGPAARLGLAGDPVTILAYANVEDAEARRRK
                     PRIVFVDGKNRIRKPR"
     gene            complement(26977..27657)
                     /locus_tag="PK634_02495"
     CDS             complement(26977..27657)
                     /locus_tag="PK634_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96637.1"
                     /translation="MSLIQEALHRKASEDGPPPPPAPPPRPGRGHPALWVVLLILLFA
                     ASGVALWYLNPSFFIRSKPESGPKPAPLAPAVPRQDEAAPAPSAPKTPAEAAAPPALE
                     PPPPPIETVAPAPVEKEPAMEAPPPVVAEKAPAAAPAAPPSELKPAEKPKDKEEAKAK
                     RWPTLKLTGVMVGHGEKSKGSAFLNGSFVEVGDRIDEVRLVEVDAKGVRLEFQGEKKY
                     LRLGQSLP"
     gene            complement(27654..28463)
                     /locus_tag="PK634_02500"
     CDS             complement(27654..28463)
                     /locus_tag="PK634_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOW96638.1"
                     /translation="MYLDFYGLKEAPFNITPDPRFLYFSARHREAYDHLLYGIEQRKG
                     FIALTGEVGSGKTTVCRAVLAALSKQVRTALVLNPCLTETQLLRAILHDFGLVPKGRD
                     RLAYIEQLNEFLLEQTRQGFNIALFIDEAQDLLPEVMEQVRLLSNLETDQHKLMQIVL
                     AGQPELQARLARPEFRQLRQRIMISCHLLPLSEEETGLYIQHRLAVAGRGEGTGFEPD
                     AVRRVHRYAGGIPRITNTVCDRAMLAGYIAGVRQIGNREVKRALSELEEAS"
     gene            complement(28473..29318)
                     /locus_tag="PK634_02505"
     CDS             complement(28473..29318)
                     /locus_tag="PK634_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96639.1"
                     /translation="MRPRRLVLVLVLLWILAFSLACRLAPRRAADPGAGDSALDRALG
                     VSRVAFGQSFYVGADDYFHRGVGHKHDVVFINSLFQRWSAAVRPSGHIHTEGYSVSEI
                     LPWLRMATAADPGNVEAFLTMAYWLTRLDRLDAAEQVLREAQRLHPRDFRLLGERGRL
                     AFRQRRDGEAARLTDAALRAWPNPLPAGDEESKLERARLLSFRAILGELRGARAPAIR
                     DFEEALQLMPANEGVRHHLALLRSGSADTALPRDALDRLFPVEHACDREEQEHEHEQE
                     QEPHE"
     gene            complement(29315..30103)
                     /locus_tag="PK634_02510"
     CDS             complement(29315..30103)
                     /locus_tag="PK634_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96640.1"
                     /translation="MKRILVMARVVWLDLLRRKDLYVMAILLLTLLFVLMSLNLFGLG
                     AVVRYILDVGLLMAWLFSLVLVVSLTARQLPREESRGTIFPLLAKPLTRAELLLGKWL
                     GSATASVFATLAFYAVIVALTALRGGAVNGPCLGQAFLLHGMALALVSAVALALSARM
                     TYGAAASMTYVLCGAAFFVAPAVPELLVHEPGPSGNVLLALYYALPHLDLFDLRQRLI
                     HDWGPAPWADVVGIAVYGAVWTGLFLLLAWLAYRTKRFRRGAPE"
     gene            complement(30100..30840)
                     /locus_tag="PK634_02515"
     CDS             complement(30100..30840)
                     /locus_tag="PK634_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96641.1"
                     /translation="MAAAIEIRDLRVEYRDKGERVEALGGLNLSVEQGTVVGFLGPNG
                     AGKTTALHVLLGFIQPSAGSAFLFGEDVGRSIARRRIGYLPEHPQLYPFLTGRELLAM
                     AGGLFGLRGPELRRRLDALLEQVGLDGASARRRIGAYSRGMMQRIGLAQALINDPDLV
                     ILDEPTGGLDPIGRLEMRGLIARLREQGKTVFFSSHELSEVELVCDRVAILARGRLVA
                     EGRVDELVRAGERLEAYFLRVVQGAGSA"
     gene            30974..31735
                     /gene="hisF"
                     /locus_tag="PK634_02520"
     CDS             30974..31735
                     /gene="hisF"
                     /locus_tag="PK634_02520"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOW96642.1"
                     /translation="MPAKRIIPCLDINAGRVVKGVRFVVLRDAGDPVQVARAYEAQGA
                     DELTFLDITASHEARDTIVEVVRAVAQQVFMPLTVGGGVRTAADIRRMLQAGADKVSL
                     NTAALLNPGIIDETAKSFGSQCIVLAIDARRRTDGPGWTVFTHGGRKPTEREAVEWAR
                     EGVGRGAGEILLTSMDRDGTQDGYDLDLTRAVAEAVPVPVIASGGVGALDHFYDGIVR
                     GKADAVLAASVFHFGTFTIPQVKEHLKNKGVEVRL"
     gene            31732..32142
                     /locus_tag="PK634_02525"
     CDS             31732..32142
                     /locus_tag="PK634_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOW96643.1"
                     /translation="MNEKEEGLELRLDFNKLTQATAANPGIIPVAVQHADTNEMILVA
                     YTNEQAFRKAVTDRSLVLWSTSRKELWEKGKTSGETFDLIEVRVNCEQNSLLYRVRPR
                     RGGICHTKNRSGQPRNCYYRRLNMETGKLENLDP"
     gene            32190..33719
                     /gene="trpE"
                     /locus_tag="PK634_02530"
     CDS             32190..33719
                     /gene="trpE"
                     /locus_tag="PK634_02530"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOW96644.1"
                     /translation="MSCFPDRKAFLEKAKQGNLVPVWRELLADQDSPVSAYEKVRDHL
                     RRQGHATHTFLLESVEGGENVGRYSFIGGTPRAILRAYGRRVTLQEPGGAEETLEADA
                     DPLDALRRYMTPFRPVADPALPRFIGGAVGFLGYDCVSVFEKRVPTRTDRALRAPDMV
                     FLVTEALIIFDRVRHTIRLIANAWIGDDAAEAYDRAVATIDELTGALRAARPSSLIDR
                     HAPAAEIEPRSNMTRGGFADAVRRAQEYIRAGDIIQVVLSQRFETDFQGDSLDVYRAL
                     RAINPSPYMFCLDLGDSAMVGSSPEVHVRCEDGRAEVRPIAGTRPRSADPAEDARLAA
                     ELLADPKERAEHIMLVDLARNDLGRVCRYGSVHVPELMVIERYSHVMHIVSDVAGELA
                     PGMDPFQLMRATFPAGTVSGAPKIRAMEIIAELEQARRGPYAGAVGYFSFGGNLDSCI
                     TIRTIILDLGKAYVQAGAGIVADSDPDREYEETRNKARGMFKALALARQFAAAREGGA
                     P"
     gene            33716..34291
                     /locus_tag="PK634_02535"
     CDS             33716..34291
                     /locus_tag="PK634_02535"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOW96645.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGADVRVVRNDAATVDEVAAMGA
                     ERIVISPGPCTPKEAGISCAVIERLGGKAPLLGVCLGHQCIGHVYGGRVVRAGRLMHG
                     KTSPIRHRGQGVLAGMPEPFEAIRYHSLLVERASLPADLEITAETDEGEIMGLQHRRL
                     PVFGVQFHPESILTQNGMQILRNFLAVPAGL"
     gene            34349..35458
                     /locus_tag="PK634_02540"
     CDS             34349..35458
                     /locus_tag="PK634_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96646.1"
                     /translation="MDENRDIYQSRGEANPPTGPSRPMDMGLDGLRRRTRATTILLAL
                     FMLGLLSAAIWLIRAQEKKLEREESGEGLPALDVSPVPLPAASQLGPSSTGQLPAADS
                     ALPLEPPTPPEPRTNAPALTPEQAAQAMTELRTGHEYLRARDLDRAEEHTRRALAIWP
                     EWTSALRVLGLIYTQRGQFEQAISVLDQAIAQDPMSAEAVNSLATAYMQKGWMGRAEE
                     LLHKVEIMNPNYWPTHLNLGLLYILKKEYGPAVEYLERAVEQVPKDAGPRNNLGVAQM
                     RIGRFEDARRNFQVIIEQSPDNPAPYFNIAISYVLEKKLDESLEWIRKGAAHCSPMTC
                     QSYLADQAFNSLRNYGPFQQLMPTLYPQLPAPPSP"
     gene            35475..36170
                     /gene="larB"
                     /locus_tag="PK634_02545"
     CDS             35475..36170
                     /gene="larB"
                     /locus_tag="PK634_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOW96647.1"
                     /translation="MNSDAQRENAIHDLGFAQVDLDRPRRRGMPEVIFCAGKTPDQII
                     GIIGAMKQAGQNVLGTRMSPEQFQAISRAIPEAAYHETARCLTLEVAPRPPAAGHVAV
                     VCAGTSDLPVAEEAALTAEWMGARVEVIRDVGVAGLHRLFKHLDTLRRARAVVVVAGM
                     EGALPSVIGGLIDRPIIAVPTSVGYGTHFAGLAPLLAMLNSCAPGITVVNIDNGFGAG
                     AAAALINRIGEPA"
     gene            36167..37348
                     /gene="larC"
                     /locus_tag="PK634_02550"
     CDS             36167..37348
                     /gene="larC"
                     /locus_tag="PK634_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOW96648.1"
                     /translation="MNLLHFDSLGGASGDMLLGALVDLGIEAAALQRELATLPIGPFS
                     IEAPKDAERHISGTRLSVHIPEHDHAGHHGRHLGEIRTIIEEAALPDPVKAMSLKAFG
                     RLAEAEARVHGTGIEEVHFHEVGAVDAIVDIVGNCLALHWLKIEAVSFSPLPLGHGMI
                     RCAHGTFPSPAPATAELLKGFPVEYVDEPHELVTPTGATLLTAWRSVERPPAGSRLLR
                     NGYGFGHRKLDGRPNLIRATLLEAPAAGAVGETCLVLECNVDDTTPELLGALSSRLLE
                     AGALDVFTVAAQMKKQRPGVLLTTLCLPGDRERLLDLVFRGCTTFGVREYLAARTMLE
                     RRFVEAQTDYGPVKIKIGRWRGEDITFAPEMEDCLRLARERNVSVRTVYEAASRARPL
                     A"
     gene            complement(37351..38211)
                     /locus_tag="PK634_02555"
     CDS             complement(37351..38211)
                     /locus_tag="PK634_02555"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOW96649.1"
                     /translation="MKTIGVLANCHKPGAAAALKRLAGSAGKMGLKLLACDETAPMLP
                     RCCAVAARELAGRIDALVALGGDGTLLRGARLLAGRAVPVLGVNLGRLGFMTSVAAKD
                     LGRALKSLVTGRYSISKRAVAECRLVRGGRTLGRYRALNDIVIGWGASSRLMTLNLRI
                     DGEQVATYHCDGLIVATPTGSTAHALSAGGPILHPDCASFVVCAICPHTLSNRPLVLS
                     DRKRLEVEVTRTAKNLILSVDGQEEPSVEPGDRVEIRRSARDVRLVQLPDYSYFSVLR
                     HKLNWRGSSV"
     gene            complement(38208..38948)
                     /locus_tag="PK634_02560"
     CDS             complement(38208..38948)
                     /locus_tag="PK634_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOW96650.1"
                     /translation="MKKIRLDQRMTDLGLAESREKAQRLILAGEVTVDGQVRDKPAFE
                     VGEAAAVAVKTPERFASRGGEKLEAAFLHFRLDVGGLVCLDVGASTGGFTDCLLQRGA
                     AKVYAVDVGHGQLHWRLRQDPRVVVMEGTNARFLYPANFSEPPAFATADVAFISLTLI
                     LPAIVRVLAGGGKIVTLIKPQFEAGREQVGRGGVVRDPAVHAEVIERIRAFGTGELGL
                     SWKGWIPSPLTGPAGNVEFLALWEKGNP"
     gene            complement(38962..39711)
                     /locus_tag="PK634_02565"
     CDS             complement(38962..39711)
                     /locus_tag="PK634_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96651.1"
                     /translation="MRRRQCGMMAAMLATALSAAAETAPMADAPGWRWMVSPMAGADR
                     NELKMRTPMGPVTVTDNGPEYGLVVMALHPNVVVNNFFFYSDVNDCDVWGDLFFANYY
                     ADAKSAVTWNAGVGYLWHEIKPEGAEIKVNVPMIKAGPVFRVKAWHLFLNPYLGYGWE
                     RVDSPGGEQDNDSFLYGLTAAWRWRMLEAGVNYYYQDSQDLDPDEDFNVLRARLIAMV
                     NRTWGVSARVDYMEHSTSDDFSGLIGPVWMF"
     gene            complement(39737..40519)
                     /gene="ccsA"
                     /locus_tag="PK634_02570"
     CDS             complement(39737..40519)
                     /gene="ccsA"
                     /locus_tag="PK634_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOW96652.1"
                     /translation="MNIKTSLLGLLIYAAMGLYAAAFAAGWRRGGRWSRRLFNAGFAV
                     ALGAVLFRGLSAERWPLQNLFEVFLAMGALVWPLALPARRLPVPGARLDPLLGFLVLW
                     PAGFVLAETLRPLPPALQSPLFAPHVLVYLGAYLLLAKAAAVSALQFAGASPEQRDAT
                     DRSAHALASWGFPLLTLGLLLGAWWGKLAWGDYWHWDPKELWSLATWLLYAGYFHARK
                     VLGRGSERLYAVLLLAGFAAIILTLSWVNLSRLFGGLHSYAV"
     gene            complement(40516..41562)
                     /locus_tag="PK634_02575"
     CDS             complement(40516..41562)
                     /locus_tag="PK634_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOW96653.1"
                     /translation="MSEFRRLLQWFALALLFGGAGLSIFGAFLGAEAATRLFNAPPMV
                     VFWIAFALALVVGLAAYPAAWRRPGLLGMHLGSLLILAGAMTGSGAGHRLAEKCGLPP
                     RVRSGVLSLRDGETSADVIDPASGRVLGALPFALRLDAFEIERYPLPSDPPSLLYGVL
                     EPEPGTPRFHWRTRVLEWKPGKTMRLAGAPIQWRVTEFTRPAENEPLSLTVELSADGG
                     PRVHTLVCRPDEPFARLALYPVFPDLTNLDRSASLILPRPEAPVREYRSRLTVLRGGK
                     ARPAEVRVNHPLHVAGYHVYQQSWGSEPEPYTVLRIASDRGWAAVWMGFVLLGAGAAW
                     HFWMRPPRRAEAAL"
     gene            41644..42213
                     /gene="nrfH"
                     /locus_tag="PK634_02580"
     CDS             41644..42213
                     /gene="nrfH"
                     /locus_tag="PK634_02580"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOW96654.1"
                     /translation="MIPWRQCLLLEGLHPGWRLAVFLMGGVLAGAVILVTHISRAASY
                     LSDEPEVCINCHVMNNAYATWRHGSHGHVAACVDCHLPHQNPVAKLAFKGMDGMRHSY
                     VFTFRLEPQVLELSHGAIPVIQENCLRCHHDQLQMVRLAAVTERRCWNCHDSVHGSVV
                     SLSASPDARRPALPSAGLEWFKKQTGDKP"
     gene            42210..43610
                     /locus_tag="PK634_02585"
     CDS             42210..43610
                     /locus_tag="PK634_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOW96655.1"
                     /translation="MNSESAKSRLPLWVGPALFLLTTGVVVLLGLLAFSITERRWETQ
                     RPAIAIQPIAEWEPDNAAWGANYPREYERYLLTRDDTTRTMFGGSFPRDYLEDDPRQV
                     LLFAGYPFSKEYRQARGHYHSVTDVTQTKRISAKNMSSCWTCKSTDVPKRMAEMGVAE
                     FYATPFSEFVGKITHPIGCQDCHDPKTMNLRITRPALREAFAAMGKDIDQATHQEMRS
                     LVCAQCHVEYYFKDGSYVTLPWAGGLGVEQIIAYYDALSFTDWTHAISKTSIIKSQHP
                     DYELYSAGIHAYGGVACADCHMPYRTEGGVKFTDHHVQSPLLNIANSCAVCHRWGEEE
                     IRSRVEAIQTKVANAKLAAEDVLVRAHFDIAAAMQAGATDEELAAARKLVRHAQYRWD
                     FVSSHNGVGFHAPQECTRILGDSAREAQEARLLVARLLAAKGISAEPKYPDLSTRDRA
                     VEVKNAFESGQPTNLL"
     gene            complement(43631..44350)
                     /locus_tag="PK634_02590"
     CDS             complement(43631..44350)
                     /locus_tag="PK634_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW96656.1"
                     /translation="MKNHVTTRWFGDWANEYDATLGKVRRHHQLLDLAARLSGVTDGD
                     RVLDVGCGTGLLSLKFLKRADCRVTAVDNSPDMRRLFQAKIDALGLRDRVRCARQDAA
                     KLRFGKSSFDIIAATVALHHVENKRPMLKTMRSILKPGGRLVIGEVDVDTTGDHADPK
                     RLARILDFLKDEIVEALKEGGVPAFSRMYDNGKKHILNLGEYCVSAGQWRALCLRAGF
                     RRVTIHDLRAFRWFKVVVAVR"
     gene            44484..46022
                     /gene="guaA"
                     /locus_tag="PK634_02595"
     CDS             44484..46022
                     /gene="guaA"
                     /locus_tag="PK634_02595"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOW96657.1"
                     /translation="MHDRITILDFGSQYTQLIARRIREQQVFCEIRRFDTPAADLAAR
                     EPQGIILSGGPASVLVPGAPAADPAIFGLGIPLLGICYGMQLMGRDLGGQVRPGRARE
                     YGSAVVRVLQPDALFRELPPTLDVWMSHGDQVEQLPPGFVALAATDTCPIAAMADPAR
                     ELYALQFHPEVAHTPRGADVLRHFLFDVCACRGDWKMADFAEESVARLRAQVGSGQVL
                     CGLSGGVDSSVAAALLHRAIGKQLHCVFVDNGLLRAGEAEQVEETFGRAFGMDLRVAR
                     AGDLFLERLAGVTDPERKRKIIGATFIDVFSEEARKLDPVRFLAQGTLYPDVIESVSA
                     FGGPSATIKSHHNVGGLPADLKFELIEPLRDLFKDEVRQLGRELGLPDSIVERQPFPG
                     PGLAVRILGAVSAERVALLQQAEIRLQEELRRLPNYREIWQSFCVLLPVQTVGVMGDE
                     RTYENVVAVRAVQSLDGMTADWFRLPHETLDRVASRIINEVRGVNRVVYDISSKPPAT
                     IEWE"
     gene            46187..49420
                     /gene="carB"
                     /locus_tag="PK634_02600"
     CDS             46187..49420
                     /gene="carB"
                     /locus_tag="PK634_02600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOW96658.1"
                     /translation="MPKRTDIKKIMLIGSGPIVIGQACEFDYSGTQACKALREEGYEI
                     VLINSNPATIMTDPATADRTYVEPITPEAIEKIIARERPDALLPTLGGQTGLNVSIEI
                     AERGILEKYGVEMIGANLEVIQRAEDRQLFKAAMAGAGLECLKSRMVTTLEEAEAAAR
                     EIGFPVIIRPSFTLGGTGGGTARDMDELRRTAAGGLKASLKHTALIEESVWGWKEYEL
                     EVMRDRKDNVVIICSIENLDPMGIHTGDSITVAPAQTLTDAEYQEMRDATIKVMRAIG
                     VETGGSNVQFAVNPKTGRMAVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LPNDITRETPASFEPTLDYCVVKVPRFAFEKFAGADPRLGVSMKSVGETMAIGRNFKE
                     ALQKAFRGLEIGVDGLGRREFDRIDPTRLEEGLATPHNERHFYIKYALEQGMPIRDIA
                     AKTHIDPWFIDQMQQIVEIERRIKDVPAGRPLPIELHREAKENGFSDLQIAALRGDRP
                     TQLEARKARTDAGIKPVYRLVDTCAAEFEAYTPYFYSSYGGLCDQTRRTDRKKVMVLG
                     GGPNRIGQGIEFDYCCVHTVMALRELGYETIMVNSNPETVSTDYDTADKLYFEPLTFE
                     DVMNIYENERPLGLIVQMGGQTPLNLARWLTQAGVPILGTSTASIHQAEDREQCRVLL
                     DELGLKQPESGTGMTEAEALAVAERIGFPVMIRPSFVLGGRAMMVAHNKADAGPFIQA
                     AFKASPGFPVLVDRYLDRAIEVDVDVLCDGESVYIGGVMEHVELAGIHSGDSACCTPP
                     HTLHPSIQRRIEDACRRIALKLEVRGLMNAQIAVKNGEIYILEVNPRASRTVPYVSKA
                     TGVPLAKIAARIAVGQKLKDMGLGERPPKVPWFAVKEAVLPFNRFAGVDPILGPEMKS
                     TGEVMGMDFSFPLAYWKSQVAAGQAVPSSGTVVLCAADPDKSWITEIGWELQDLGFTL
                     AATAGTAAALKAAGVESRVLQKVRENKSPNILDLMRANEIVLLINTPTGIKDRVDEVH
                     IRSEAILRSVPIVTTEAGARATIAAIRHMRGQAWDVHALQDFHPAKA"
     gene            complement(49439..>50030)
                     /locus_tag="PK634_02605"
     CDS             complement(49439..>50030)
                     /locus_tag="PK634_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96659.1"
                     /translation="ATTKFGGELDTYVDMTSFGLAPAFLAHQLVLKDAGIWGLVITCA
                     IVVSGVSRLSRFRVVDPFRGQRGYLGLPITTCAGWIALSVLIAESGYISDDVFSLSHG
                     PTATFIWGCVLAMVLLQVSHVHYNKPTKGLRGMAPAVLLITALFFNSRWAVAAAFTLN
                     AFGFYFAFLSPFFNRRHAMLVAYEEEKEDEPAPLPR"
BASE COUNT         8174 a        17434 c        16935 g         7487 t
ORIGIN      
        1 cgtgtgtcgc gcttccgcgt cggcgcgcgg ggcggcagcg tcaccctttc caagtccaac
       61 ttcctgatca ccctcgcgcc ctacttcttc ccgctctaca ccatcctggt gatcgccctg
      121 cacggcctgc tggccctgtt ctacgacctg cgggcctacg aaccgctctg gctgggattc
      181 gtcgggctga cgtggagctt ccacctgacc ttcacggtca cgacgctccg gcagcgccag
      241 ccggacatcc gcattcacgg ccggctcttc tcctacgcgc tgatctacct gctcaacctc
      301 ctcggcgtcg ggctcggcat cgtcgccgtc gccgccccca ccctcgcgga attcggggcg
      361 cagctgggcg cggatatgct gaacctctgg gaactgggcg tccgcctctc gcgcggcgcg
      421 caggcctggc tgcaggcgca cgggccggcc cggccctgaa cgcggggcgg ctatggcggc
      481 ggcccgccgg gttccatcat cattacaact tgcccggcgg ataccgcctg gttggattgc
      541 acgaggatgg ctgtcacccg gccgccggcc gggctccgga tccagcgccg gttgtcctcg
      601 cgctgaacct gctgaatgcg gcgcaaggct tcgttccggt cttccgaggc ttcttccatc
      661 gcgacccgcg cggcctcttc gtcgccgggc gatgcgggac ggccggtctc ccggagctgc
      721 acttgcttga ggtactcggt ccgcgcggcg gcaagggttg tttcagccga cgccagggcc
      781 tgccgcgatt cttcaacttc atccctggcc ggcgccagca cggccacgat gtcgccacgc
      841 cgcacgggat cattcggccc gacgcgcagt tcgctgacct gcccggccat ctcggacacc
      901 gcgggcacag acggaatcga tgaacctgcg gcggcatgcc cgggttcgac aggactcgct
      961 tcccccgccg ggaccggttt cgaagatgga gcgatcgatt cggggatgac ggcctctttc
     1021 cggccgcacg cggacaagag cagtccaacc gccatcgcga ccccgttctt tgtgctaggc
     1081 catcccttca tgccgactca tactaccctt ctccatccgg gctggacaga ggcccgggga
     1141 gcttaaaacg ataaagcgtt taactaagcg ggaatgcagg gttatggtag tgtctacatg
     1201 ccgcagcgca atgtaagagc gcttcttgcc gtcatggcct ttggggtgct tgccctgccg
     1261 gcgctcgcct ccgtgggcaa cgtgacgggg atcgcgcgca acggagatac gttgctgatc
     1321 tcagccggcg tggacacggt cgtggtccag gcctgccgcg cgaacctcct gctcgtggac
     1381 taccgccctt ccgggagaac cagtgcgaac accctgatga tcgccacgac caactggccc
     1441 gccgccgggg ccacgatcaa caccaacggc gacccgatcc tgatcgacac gacgaacctg
     1501 cacgtcgaga tcgcgcgcgc gccgctccgg atcgccgtgt acgacgccac cggcacgaac
     1561 ctgctgatcc gggagcaggc ggcggaagga ttgttcgccg acggcgtgcg gtttcaccac
     1621 aggcccggct ccgatttcta cggcatcggc gggtacaacg cctgggacga ctcgagcgcg
     1681 ggcatgctgc gcaacagcgg cggctgggtg gaggccggtt accaaggcga cgccggcgcg
     1741 ccgctggcct ggacccggca gggcttcggc gtgctggtgg attccgacgg cgggcagttc
     1801 accatcggcg gaacgaacac ggtcttcgag tactgctcca agaccaacat cctcctctac
     1861 atcgccgtcg ggccgccggc cgacatcctc gccgccgcgg ccgaggtcac gggcaaggcg
     1921 ccgctgttcc ccaagtacgc gatggggttc gcgaacacgg agtgggacat cacgcagggc
     1981 gagctgacga acatcgtcaa cggctaccgc tcgcgggcca tcccgatcga ccactacatc
     2041 gtggacttcg actggaaggc gtggagcgag gacaactacg gcgagtggcg ctggaacacg
     2101 accaagttcc ccggcggccc gtccggcgcc ctgcattcgc agatgcgggc cgcgggcatg
     2161 cacctctccg gcatcatgaa gccgcgcatt ttcgtcgaca ccgtccaggg caactacgcg
     2221 acggcgaacg ggttctggtg gcccggcagc agcgagtacc aggactactt cacgggcggc
     2281 tgggtcaacg acctgaattt cgcgctgtcc gcctgccggg actggtactg ggaccatatc
     2341 acgaacgcgt tcagcaccgg gatcaccggc tggtggaacg acgaggcgga ccagaagggc
     2401 ggcggcgggg gcttcttcga caactgggaa ttcatgaaca tgcagcgctc gctctacgaa
     2461 ggccaacgcg gctactcgac ccagcgggtg tggtcgatca atcgcaactt ctacctcggc
     2521 gcccagcggt acgcctacgc catgtggtcg ggcgacatcg acacgggctt ctccgccatg
     2581 gccgcgcagc gcgagcgcat gctctcggcg ctgaacctcg gccaggcctg gtggggcatg
     2641 gacatcggcg ggttcaacgg cggcgatccc tcgtccgaga attacgcgcg ctggatccag
     2701 ttcggagcgt tcgtcccggt cttccgcgtg cacggccagc agtacgccca gcgccagccg
     2761 tggatctacg gctcgacggc cgagacggcc gccgtgaagg cgatccgcct gcgctaccag
     2821 ctgctgccct acatctacag ctgcgccggg cgcgtgacgg agaccggcgc ggggttcgtc
     2881 cggccgctgt tccacgattt cccgaccgac gcgaacgccg ccaactacaa ggacgcatgg
     2941 atgttcggcg acgccctgct cgcggcgccc gtcgtcgccg agggccagac ggcgaagagc
     3001 atctacctgc ccgccgggac atggttcgac tacttccgcg gcacgcgcta cgcgggcggg
     3061 caaaccctct cctattcgct caactcgacg acgtggacgg acattcccct cttcgtccgc
     3121 cagggcgcca tcctgccctc gcagccggca atgaactacg cgggggaatt tcccctgaca
     3181 aacgtgtcgg tggacatctt ccccgatacg gcgaccagca ctttcgctta ttacgacgac
     3241 gacggcacga gctacgcgta cgagaccggc cgcctgttcc gccaggagtt caccgtgcag
     3301 gaccgcggcg agagcatggc ggtggacatc tccgcggctt cgggttccta cgtcccgccg
     3361 ctccaaacgt acttctgccg gttgtactgc ccgaccgcgt cggcggtcac catcaacggc
     3421 gcggcggcca cgccctacgc cagcgtcgcg gcgctcgaag cggcggccgg cgagggctgg
     3481 gcgctcggca cgaaccggtt cggaccgctc gtcgcggtca aggtcgccgc cggcgccgcg
     3541 aagagcatcg tggcctccaa caacgccgtg gccacgccgg ccttcgcgcc gccgggcggc
     3601 acgttcacgg gcgcggtgct ggtcgcgatc tcgtgcgcca cggaaggcgc ctcggtctac
     3661 accacgaccg acggcagcga gcccgacgag acggacgcgt tgtacgccga gccactcctc
     3721 ctcgccgtct cgaccacggt caaggctcgc ggcttcaagg ccggacggac gcccagcgtg
     3781 gtcgccgagg cgtcgttcgc gcgcgacgat aacctgctgc gcaatggcgg gttcgaggcg
     3841 cagggctcat cgacgaacaa cgcgctctac tggaccaccg gcgagccgga cgagcacggg
     3901 gaaacgtggg gctccggcct gcgcgtgagc tggcgcagcc actcggatac gtggcagggc
     3961 gccatccgcg gcacgtgggc cggcgcgggc agcgaaggcg ggttctggca ggaggccccc
     4021 gccgtgccgg gccgcgccta ccggttctcc gcatggctct gggcggattc cgactgggcc
     4081 ccctcgctcc aggctttgaa gatcgagttc ttctccgggg cgaccatggg cgagacgatg
     4141 ctcgtggccc acacgaacac gttcacgggc gtcgggccga catggaccaa ccgcgcggtg
     4201 caggccacgg cgcccaccaa cgccgactgg gtgcgcgtgg tcgtggtcgc ctccggcgtc
     4261 agcggaagcg gcgcgctgca attcgacgac ctgcgcctcg acccgaccaa cgtgtgcacg
     4321 ctgacgatcg agtcgccgca cggcagccct tcccccgcca tcggcgccca cctggtggac
     4381 cagggccgca cgatcaccaa cagcgtcaac agccccgtca cgagcggcga gacccagttc
     4441 gtatgcgccg gctggaccct cgccgagcac gccccgacgt ccggcgcggg cgcggtgatg
     4501 acgatgaccg tgaccaacaa cgccgtcctc acctggcgct ggaccaccaa cctcctgaat
     4561 cccagctacc tgtccttctc gagcctggcc tatacttccg ccgaggcgga cgtgaccgcg
     4621 cgcctggcga tcgtccggtc cggcggcagc aacggcgagg taagtgtgag ttaccggacg
     4681 accggcggat cggcctccgc tggcctcgac tacgcggcgg tcacgggaac cgtcgtgctg
     4741 gccgacggcg tggtcaccgg ctcggcggcc gtcgagctgt tccacgatct ccagttcgaa
     4801 tccggcgaga ccgtcgaggt gtcgctcttc aacccgtcgg gcgcgacgct ggcggcgccg
     4861 accggcgcgg tgctgacgat cctggacgac gacgccgact tcggcacgcg cacgctttcc
     4921 gtgatctcca cgcacggtca acccgatccg ccggccgggc tccagtcttt cgactacggc
     4981 atccggctga ccgcttccgt caccggcacg gcggaacagg ccaccaccca gtacctgtgc
     5041 atcggctggg ccggcacgga aagcgtgccc gcatcgggaa ccaccaacac aaccccgtcg
     5101 ttcctgctga cgaacgattc ctcgatcgcc tggcaatgga ccacaaacgt gtacttctcg
     5161 cccgccgccg gcccgaacgg ctcggtcggc ggggatgcct ccggctggta tccccttggc
     5221 gcgggcgtcg tcgtgaccgc gacgccggtg acggcctact ccttcgccgg ctggaccggt
     5281 gacgtgccgg caggccacga gttggacaat cccctcgccc tgaccctcgg ccaggcccgc
     5341 gccgttacgg cgaccttctc ggcggacgcg ggcgaaaacc tgctcaacaa tcccggcttc
     5401 gagcaggccg gcacctcctc ggtccgggcg ctctactggg accagggcga tcccgacacg
     5461 cacggcgaga tgtggggcac ggccgcccgg gtggattggc gaagccacgg cggcacatgg
     5521 gaaggcgcca ttcaaggaca gtactccgga gccggcacgg aaggcgggtt ctggcaggag
     5581 gtcgtcgcca gccccgggga agaataccgg ctgtccggct ggttctgggc ggacgacggc
     5641 aacccctacg gtccgtgggg cgcgggcgag caggtgatca aactcgaatt tatcgaaaac
     5701 ggggtcctgt tgtccgccgc gaccggtacg ctggccggag tcgggcaggc ctgggaagaa
     5761 cgctccgtga gcgccctcgc gccgaccaac gcggattggg tgcgcgcggt gatcctggcc
     5821 cgcgacatca gctacgacgg ctcgctgcaa ctggacgaca tgatgctgga aatggtgccg
     5881 acgctggact cgcccgtgat tcaacacgtc gtcccggtgg acggcacctc ctgcgtgctg
     5941 gaatggatcg gtgtcgccgg ggcggacggc tacatcgtgg acgtggccac gaacggggcc
     6001 ttccagccgg agagccgggc cagcgacctg ttcatctccg aatacggcga gggtacgggc
     6061 agcgagcggt acatcgagat ctggaacggc accggagctg aagtggacct gacggcgtac
     6121 cgcctttggg gcatcagcgc gggcggcagt tggttcgagt cctccctggt cttgagcaac
     6181 acgctggccg agggagccgc ccacgtggtg gtgaacgaca actgcacgag cgccgtactg
     6241 cgggccgccg ccgaccagtt cgcggccaac gcggcgccca tgaacttctc gggcgacgac
     6301 gcgatcggcc tggccaaggt catcggcgtg gtgaccagtc tcatcgacgc cgtcggcacc
     6361 accggcggcg atcccgggac cggttggggc gtcgcgggcg tggccgatgc cacggccaat
     6421 catgggctga tacgcctcgc ctcgatcacc gccggcaaca cggactggag cacctgcacc
     6481 aacgaatggg ccgtgctccc ggtcaactcc tggacgaaca tcggccagca caaaatggat
     6541 gtcacgccca gtgacttcgt ccccgggtac gaggagcggc ccacccccgg cgtcacgagc
     6601 ctgaccgtca cggggctcgc cacgggcgtg acgtactact tccgcgtgcg ggccaccaac
     6661 gcggaaagct tcagcgtctg gtcggagacg ttcgaggcgg cgctggcctc cggttacggc
     6721 atcctggcca cggccggtga acacgggacc gtatcgccct cgggactggt catggtcgcg
     6781 gcgggcgcgg gcgccaactt cgtcgtgcag gcgggccctt actacgtgat cgccaccatc
     6841 ctgaccaacg gcgccgcctt gaccctgccg cccggcctga ccgtgtacac cgccgaatgg
     6901 agccaggtct tcgccacggg gaccttccac tccacgttcg cggagcggca ggccgatgcg
     6961 cacgcgacgc cttggtggtg gctggcgacg cacggtttga cgaacgagga gacgagtttc
     7021 aacgaggccg agttgggcga cacggacacc gacggccacg cggcctggtc ggaattcgtg
     7081 gccgacaccg accccggcga ttccaattcg gtgctcatgg cgcatcccgg cggcgccgcc
     7141 ccctccggcg cgtacacgat ccgctggccc ggctccacgg gccgctacta tcacgtctac
     7201 cggggcacca acctggcgct cccgttcgac cgcgtggccg gcaacgtgcc cggcatcgcc
     7261 cccgtcacgg tccacacgga tgcggtcttc caggggcaga gtcccgcgta ctaccgggtg
     7321 cgcgcgctcc tcgcgccgga agagtaaaaa ccgggcggga aggcttgact tccgcccgtc
     7381 tttccctagt gtgttgcagc attcggttcc ggcgtagctc aatggtagag cgggtgactg
     7441 ttaatcacta ggttacaggt tcgagccctg tcgccggagc cattccatgc gcgtgtcttt
     7501 tccgaacaag gatgtcgttt tcccttgcaa tccccgggta ttagcggatt ctaggggctt
     7561 caggttcctg caagagaagg agccttacaa aggagattga tgatgagcat cgttcgttgt
     7621 ctggtcgtat tggccgccgg gctggctctc gtggccggct gcagcaagag tgagcaggaa
     7681 ccggccgccc cggctgcggc gcccgcttcc gaacaggccc ctgccgtcgc ggaacccgcc
     7741 ccggctccgg cccccgcgcc gatgatcgag gaagtggagc agaaggccga ggaggtcacg
     7801 gagaccgcgg ccgacgtagc cgcccaggcc gaggccagcg tcaaggaagc cgccggcgag
     7861 gccatggaag ccgcctccgc cgccgtcgag accgccaagg acacggccca ggacgccgcg
     7921 gcggccgcgg gacaagccgt cgaggaaatc aaggccgacg tcatgccggc cgcgacggaa
     7981 acccctcccg ttccggcccc ggccgaaccg cagccccagc cgtaagataa cgtcaaattc
     8041 tgaaaaggcc gtccccgcgc ggggacggct ttttctttct ccaactcgcg ttcagttttc
     8101 ccggggcgcc acgtcgacgg acggcccctt gatttgatcg aaataggcgg cgaaatacgc
     8161 ggccagcgcc ggatcccgaa cgaccacgca ggtttcgttg cggcgctcca gggcgtcata
     8221 tccccaattg acggagccga tgaccacgcg gtcgtccgcg acaattacct tggcatgcgt
     8281 cgtgatgttc tcggcgtcat cgtgtaccgc cacgcccgac gcggccaggt attgccgggc
     8341 ggcgtcgttc accttgttca gcgtgtcgtt gtagtcgctt agatccatga tgaccgccac
     8401 gtccacgccc cgccgatgcg ccgtggacag cgattcgacc aaccggttca ccttgctgtc
     8461 cttgtatttc ggctcgtact tgatgccgta catgatgacg cggacggtcc gggtggcgga
     8521 gtcgaacagc gacagcactt cgggaaaata ctggcggttc acgatggtct tgacgccgcc
     8581 cgattcgacg gacggcacgt ccggctccgc gaagctgtct gcccgcagcg cggcaatgta
     8641 gcgctggaac gccccggcca ggaccggatc gtccaggagg aggttggtct cgttgttatt
     8701 gtccatcgag ttgccggtca ggttcgtgga tcccagcagc actttccgcc cgtccacgac
     8761 gaccaacttg ctgtgcagca tttttttcgg ggtgtcgagt tcggcctcgg cgcccaacgc
     8821 gcgaagccgc tccacgcccg ccgcattgaa ttcgatttta ttttccacca gggcctgcac
     8881 gcgcaccccg cgcgccacgg cggcggccag ggccgcttcc acccggagca cggtcgcgtc
     8941 gtcgtgatac tccaacgcaa ggaactcgat cgaattggtc gcgcgctcaa tcagatcgag
     9001 caaaacgggc aggtactccc gattaaccac cggccggacc gcgggcgcgg ggacgtcgcc
     9061 ggcgtgagcg gccgccgcga gcatcagcag cgcgagcagg gcccccgggg aatgagcccg
     9121 tgccggaccg ggccttggcg caaaggctcg gatcaaagac gcggacgcca cggcgccaac
     9181 gaggttggag accaggtccc agccggtgtt gacgtacccg ccgacgttgg tctcggggac
     9241 ggtcagcgtc gccgcgaatt ccacgatctc gttcagcgcg ccgaacccca tccccgcggc
     9301 gacgcagagg atcagcaggc cggcggtcgg ccgcggttcc gccacgccgc gattccgcag
     9361 cgcggcgcgc aggccctgcc agcataccca ggtcgtgacg ccgaacccgc aggcgtgcac
     9421 gaccatgtcg tacttcagcc ggcccgggac gatccaccag ctgtacagca cgcggatctc
     9481 gcccgcaatc ggccagcttt ccggcacggg caccagcccg ccggccatgt gcagcaggcc
     9541 ccagccgctc aggccccaga gcgcgcccgc cgtaagccgg acgcaccggt ggagcaaacc
     9601 gatcagcgcc atcaggacgg ccatcacgcc gatgtagagc atgaactccg cgttggaagt
     9661 ccgcgtggtc accagggccg cgatcacggt gtaggaaagg gaaaaaacgg ccacggccca
     9721 aaaggggtct cggcgattca tgacgggcgc atcctccggt tcgatttaac gacattctaa
     9781 caaagccctc acacgccgcc aatccccggc ccgcagagtc gccggcggaa ggtacgcgga
     9841 tcgttcaacc cggaggtcag catgccgaga tggaagcttg cgcgattcgt cctggccctg
     9901 gcggcgcccg ccgcctttgc ccaggttccg ctggacgcgg gtctgccggt ctaccagccg
     9961 gtggaaggca tctccgggac gatcaagagc gtgggctcgg acacgctgaa caacctgatg
    10021 acgctgtggg cggagggctt ccggcggatg taccctaacg tccaggtcga gatcgagggc
    10081 aagggctcgt ccaccgcccc gccggcgctg atcgagggca cggcgacttt cgggcccatg
    10141 agccggccca tgaaggaggc ggaagtcgac gggttcaaac agaaatacgg ttacgccccc
    10201 ctggcgctga agaccagcat cgacatgctc gcggtgtacg tgaacaagga taatcccgtc
    10261 gaaggcctga cgtttgccca ggtggacgcg atcttctccc gggaccgcaa gctcggccat
    10321 cccgaggacc tcgtcaactg gggccagttc ggcctgcccg gggaatgggc gacgcggccg
    10381 atcagcctgt acggccgcaa cgccgcctcc gggacctatg gcttcttcaa ggagcatgtc
    10441 ctgggcaagg gcgactacaa ggactcggta aaggagcagc ccggcagttc agccgtcgtg
    10501 cagggcgtgg cctccgaccg cgggggcatc gggtacagcg gcatcggcta caagaccgcc
    10561 gacgtacgcg ccgtgccgct ggcgatggca gagggcgagg acttcgtccc cgcgacgccg
    10621 gaccaggcct acacgggcga ctatcccctc gcccgcttcc tgctggtcta cgtcaaccgc
    10681 cggcccggcg cggagctgga tccgctccag cgggagttca tccgctacgt gttcagccgc
    10741 cagggccagg aggacgtcct gaaggacggc tacttccccg tcaccgcggc catggcccgc
    10801 gaggccctgc aggcggcggg gatcgagtaa tccggatcat cagcccactc atgcaacccg
    10861 cgcgaaacag gaagacccgg tgggccgtgc gcgcctcgga ctcggtttcg cgcgggctga
    10921 tcaccgtcgg cggcatcggg accatcgtcg cggtgttcac ggtctgccta ttcctcgtcg
    10981 cggtcgccct gcccctgttc cgcccgccgt cgatccgcgg cgcggccacc cggagcgccc
    11041 ccgcgcggct cccgccgctg ctcatccagc cggacgagga cgggcgccgc gcctgggcgc
    11101 tggccgccga cggatcgctg tccgcgttcc agatgaacga ctcgtcggcc gccgtcacgg
    11161 aacgggtatt ctccaacgcg ccgatcacgg ccgtctcgac gtcgccggac ggcgcgaacc
    11221 ttgcgttggg cttcgaggac gggaccgtgc ggcgcggccg ggtgggatgg gcggaagggg
    11281 ccgcccgggc ggacttcggc gaggccgcgg cgttttcgcc cccgtctccc gtccggctgc
    11341 tcgcgcacgc gggcgagtca ttctgcgcgc tgaccgccga cgggcgcctg ttcttcgaga
    11401 agatcacgga gaggaggaac ctggtcacgg gcgaggtcac gccacgccgg cgcgcagcgg
    11461 tcctgccata cgaaccccga acgaacgggg agccctcctt cctcctcctt tcgggactgg
    11521 cggactgggc gctgctggcc tggcccgacg ggctcgccat ccgttacaac acgcgccagc
    11581 ccgagaaggc cttcatcgag gagacgctga acctgctcga cgatccggcg ctccgcctga
    11641 ccgcgatgcg ctgcctgccg ggccagacga cggtcctctc gggcgactcc ggcggccgtg
    11701 tccgggcgtg gttcccggtt cggccggacg gcacgaacgc gcttacgcgc ctggtcgccg
    11761 cccgggaatt ccggggcgcc ggagcgcccg ttgcctgcct cgccaccgcc ggccgcatgc
    11821 gcgtggttgc catcggctat gcggatggct cggtgcgcgt cggccacgtg acaagcggca
    11881 agacgctggc caggcttccg gccgggccgc gcgggcccgc ggcggcgctg gctttccttc
    11941 cccgcgacaa cggcctgctg gcgatccggc ccgacgggct ttccctgtgg cggctggacc
    12001 cgcgccatcc cgaggcgacc tggaaggcgc tctttgcccc gatctggtac gagggcgccc
    12061 cgcggcccgc gcatacgtgg caatcctcgg cggccacgga cgatttcgag cccaagctcg
    12121 gcctcgtgcc gctgatcttc ggcacgctca aggccaccct gtacgccctg ctcttcggcg
    12181 tgcccgtcgc gttgctcgcg gcggttttca cgagcgagtt cctgcacccg cgcgcgcgtc
    12241 gcgtcgtgaa gccggccatc gaaatgatgg ccagccttcc gagcgtcgtg ctcggatttt
    12301 tcggcgccat cgtcatcgcg cccttcgtgg aaacagcggt cccggccgtc ctcgtctcgc
    12361 tcgtgaccgt tcccgtcatg ttcctcgcgg gcgcgcatgt ttggcagttg ctgcccccgg
    12421 cccttacgct gcggcttgcc tcgtggcggc ttctccttct ccttctcctt cttcccctcg
    12481 gactcgcggc agccgccggg ctgggcccgg tggccgagcg gctcctgttc gccggggata
    12541 tgcggcgctg gctggatggg caactgggag gcccggccgg cggctggttc ttcctgatgc
    12601 tggccccggc ggcgctggcc gccgcgctga tcatggcccg cgcgatccgg ccccgtctcc
    12661 agggcgcggc gcgctcctgg cctcggagcc gggtcgccgc ggcggatgcg ctggctttcc
    12721 tggcggacct ggccctcacc ggcggcctgg cctgggcgtc agccggcctt cttacggccg
    12781 cgggatggga tccgcgcggc gcagcgctcg gcacctacgt gcagcgcaat gcgctgatcg
    12841 tcgggttcgt catgggcttc gccgtgatcc ccgtcgtcta cacgatcgct gacgacgcgc
    12901 tggccgcggt gccggaccat ctccgctccg ccgcgctggg cgccggcgcg acgccctggc
    12961 agaccgccgt caccgtcatc atcccgacgg ccatgagcgg gctgttctcg gcggtgatga
    13021 tcgggctcgg ccgcgccgtc ggcgaaacca tgatcgtgct catggcggcg ggcaacaccc
    13081 cgatcatgga ctggaacccg ttcaacggct tccggaccct ctccgccaac atcgcggtcg
    13141 aactgcccga ggccgtccgg aacagcaccc attaccgcgt gctcttcctg tccgcgctgg
    13201 tcctgttcgc cctgaccttc gtcatcaaca cggcggcgga actggtccgg ctgcgcttcc
    13261 gccggaaggc ggtggcgata tgagcggcgg acgcgcagct ccgcgtcgcg aacgcaccgc
    13321 cctcttcgcc cagggcgaac cgatgatctg gctgaccggc ggcgggctcg cgctcgcgct
    13381 ggccatgatc gccggcctgc tggcgctggt ggtcgtccag ggctcggtca cgctgtggcc
    13441 cgggcccgtg acgcgggtcg aactgaacga cggccgcgcg gtcatgggcg aagtggcgcg
    13501 ctcggagtcc gtcggcgcga ccggaggcga cgggcccgtc cggcgacgcc tgttccgcac
    13561 gggcaacttc gagctgaccg gcgagcattt ccaatgggtc gccgattccg acatccgcgc
    13621 ggaatcccgg cccgaatggg cgctggtcgt ggagcggaca gcctggggcc ggttctacgg
    13681 cgccccccag gcgttcctgc tcgacgggcg cgcggcggcc tccgacccgg cgtcggcgtg
    13741 ggcgatgtac gaacaacatc gcctggcggt acagcgccgc ctgcgcgagc ggcagcggct
    13801 ggagagccgg gacatcggcc gcgtaaaccg ccgtatcgaa agcgcccgcc tcgcggtcaa
    13861 gtcggcggag ttgcggcacg gcatgggctc ggtcccccac cgggaagccg tggcgcggtt
    13921 cgaggccgtc gaacgccagg gcttggcgga gcacgcccgg ctgcgcgggc gcatcgaggc
    13981 cctgaaccgc gagaacgcgc gctttcagct ggcgctggcg accgcggacg gctgggagca
    14041 ggccatcccg ctggcggata tcgtccgggc ctgcgcgccc aaccggctgg gcctcgccgc
    14101 gaagtgggcg gtctacggct cccgctggct ggaattcctg tccggcgcgc cgcgcgaggc
    14161 caacagcgag ggcggtgtct tcccggcgct gttcggcacg gccctgatga cggtcgtgat
    14221 gtcggtggcc gtcgtgccgt tcggcgtcat ggccgcgctc tacctgcgcg agtatgcgaa
    14281 ggccggcccg ctggtcagcc tcgtgcgcat cgcgatcaac aacctcgccg gcgtgccgag
    14341 catcgtcttc ggcgtcttcg ggctcgggtt cttctgctac attgtcgggg ggtggctcga
    14401 ccggtccttc ttcgccgcga acctgccgaa cccgacgttc gggaagggcg gcctgctgtg
    14461 gtcggcgctg acgctcgcgc tgctgacgct gcccgtggtc atcgtcgcga cggaggagtc
    14521 gctggccgcg gtgccgaact cgatgcgcga gggctcctat gcgtgcggcg ccggcaagtg
    14581 gcagacgatc cgccgcatcg tcctgccgcg cgcgctgccg ggcatcctga ccgggacgat
    14641 cctggccatg gcgcgcggcg cgggcgaggt cgccccgctg atgctcgtgg gcgcggtcaa
    14701 gttggcgccc gagctgccgg tggacgccgt cgcgccgttc ctgcaccccg agcgcagctt
    14761 catgcacctc ggcttccaca tcttcgatct cgggttccag agcccgaaca gcgaggcggc
    14821 gcggccgatg gtcttcaccg tcacgctgct gctgatcgcg ctgatcttcc tgctgaacct
    14881 ggtcgcggtc ctgttgcgcg cgcggctgaa gaagaaattc gtcacgggag cattctgacc
    14941 atgaacaccc tcacggccac cgacaccctg accctcgagc cccccgcgcc cgccggcgac
    15001 gaccgcctgg ccgccattgc ccggggcggg caattcgacg ccgtcgttca tccggcggtc
    15061 gaccgggagg atgcgacgct tgaggtccgc gacttctgcc tgtggtacgg gccgaaaaag
    15121 gcgctgcacc acatcggcat gcgcatcccg cgcggcaagg tcacggcgct catcgggccg
    15181 tcgggctgcg gcaagtccac cctgctgcgc tcggtcaacc ggatcaacga cctgctcgat
    15241 accgtccgca tcgaggggga catgctcctg aacggcgact ccatctacgc gccgggcgtg
    15301 gacgtgatcg agctgcgcaa gcggatgggc atggttttcc agaagccgaa ccccttcccg
    15361 atgagcgtgt acgagaacgt cgtgtacccg cggcggatcg acggcgagcg cagccgcgcc
    15421 gcgctggccg aggtctgcga gcgcagcctg cggcgggccg cgctctggga cgaggtcaag
    15481 gaccggctgc aggagagcgc catgagcctg tccggcggcc agcagcagcg gctgtgcatc
    15541 gcgcgggcga tcgcggcgga gccggaggtg ctgctgatgg acgaaccctg ctccgccctc
    15601 gatcccatcg ccacgaacaa gatcgagaac ctgatcgacg agctgcgcgg ccgctacacg
    15661 atcctgatcg tcactcacaa catgcagcag gcctcgcgga ccagcgacta cacggcgttc
    15721 atgtacctcg gccacctggt cgagtacggc ccaacggccg acctgttccg caagccgcgc
    15781 ctgcgcgaga cggaggatta catcaccggg aggttcgggt gagcgccggt tccggattga
    15841 ccccgaagcc cgcgggccct aacaaggaga cgcgaccatg atgattcatc tccacaagga
    15901 aatcgaaaac ctgaagaagc ggctgctctc gctgtgcgcc gtcgtcgagg acaatctccg
    15961 gcagtccgtg caggccatca cggagcgcga cgaccggcgg gccgagcagg tgatcggccg
    16021 cgacctggag atcgaccagg ccgagatcga ggtcgaggag gagtgcctga agatcctggc
    16081 cctctaccag cccgtggcga tcgacctgcg gttccttgtc gccgtcctca agatgaacaa
    16141 tgacctcgag cggatcggcg acctggccgt gaacatcgcg cagcgcgcga tcttcctgaa
    16201 ccgggagctg ccgatcgagg tgccgttcga cctgaacggc ctgtcggaga agacccagac
    16261 gatgttccgg ttgagcctgg acgcgctggt caacctcgac gcgagcctcg cgcggaaggt
    16321 ctgcgcgatg gacgacgagg tggacgcgat caaccgggcg atgtacgacg gggtgaaggc
    16381 gatgatccgc cgccaacccg agcatctcga tgcgctgatc gcgctgctct cgatctcacg
    16441 ctacctcgag cggatcgcgg accacgcgac gaacatcgcg gaggacgtgc tctacaccgt
    16501 gcagggcgcc atcgtccgcc acgcccgcga attcaagcgc ctgccgccgg aggacgctcc
    16561 gcccgcttcg gtgcgtcgag attgacgacc gtcctgttcg cggataccct gccggtagtt
    16621 gcgcggggag acgtgttcat ctacccaaat ttggaatacg caggcgcgag gcggagggaa
    16681 cgacggctcg cgtccgtgat cctccggctg gccagccttt ttttcgtccc gcttgccccg
    16741 gccgccaccc gattccttcc cgtccccggg ccgccgctga ctcacttgcg gacggactac
    16801 cagaggcctt tcctctacgc cctgcaagag ggcgccacca gcacggatgc ctgccgtctt
    16861 ctcttcatcg acacgcggac cgatgccatc gcgaccaact gcccggcggg cctcaatgcc
    16921 agcgacctgg cgattgttta tggggacacc cgcctgtacg tcgccaactg gaagcaggcg
    16981 gcttgctgca cgttcgatct cgacgcccag gcctctgccg gccccgttgc gctcccctcg
    17041 gaatgccggc gcttgagcgg cggaagccaa gggcggttgc tcgcggaaaa agagggcgtg
    17101 accacgagct cggacgtgct gctgctggaa tccaccagcg gcaccacccg ggcctcgtgg
    17161 agcggccggt tgggcgacgg cgatatcgaa cccacccgaa ccaattactt ccatgcccag
    17221 aacaggcagc ttttccgcct cgtcgtgacc acgaacgcga ttacgtccga cggcgcctcg
    17281 gaaggctttc ccgaggtcgg cggctgggac gacacggtcg tgctgtccgc cgatgggcag
    17341 accattttct gccaaggccg cgcgatcgat cgccgtcttg aagtggtccg gacgtttccc
    17401 gaacccatcc gggccgtgag cctgcacggc gaggtcgcgg tgggcacgca gcaggtgttc
    17461 tctacggcca cgggcgatcc gcttcttgca ctgccggcgc cgtcgtcggt ctcggcattc
    17521 tccggcgacc agcgaaaatt gtacgtgtat gactcggtgt caaacggtct gtacgtggtg
    17581 gatctgacca acgagatcgc gctgtcgaat gcctggctcc agcccgaacc ggccgacggc
    17641 gcgctcatgg gcaggatgcc gcctctgtgt tggacgccga cacccgtcgc ggacggctac
    17701 gacgtgtacg gcggcgcgat cagccaggaa gtggcgctcg cgggcacagg gtccgtcact
    17761 tttcttggcc ggacaacggc cggccgactg acccccacct cggccctgcc ggtccagtcc
    17821 gagtatttct ggcgggtgga cgcgctcaca tcgtccggag ccgttcaggg tgctgtctgg
    17881 cgttttacgt ccgggcccgt ggtcgtccag ccgggtgaga tcaatgccct gacgctggag
    17941 cacgcgccga cgctggcgac gcaactccag gttcgggccg tggcgaccaa cgtttgggcc
    18001 ctgacggcgc aagcgccctg gctgtcctgc acccccacgt cgggcctggg cgcctcgctg
    18061 gtcagcgtcg cgctccggcc ggagggcctg tcgcctggcc ggcatgaatc cgcgctccgg
    18121 gtggaatcgg gcgcattcac ggacaccgtt ccggtggtgc ttcaggtatt gacccaggag
    18181 gtcgtgcggg tcgcggccga ccgcaagcgc gcccgcgtgt atgccctgca cgcggcggct
    18241 gaccagctca gcgccgtgct ggctttcgac agcgcctccg gcgttatgac cggcctgttg
    18301 tttgtcggac gcggcgcgtc ggacatggaa ctcgatcccg ccagcgacga tttgtggtgt
    18361 gtcagcgggt tcgattcgtc gctttcgcgc atcgcgttat ccacgttcga ccacgtggac
    18421 acgattccat tcaacctcgg cctgtcgggc gcgcaggtct ccatctacca ggtggcgcct
    18481 ggcccctcga actatgtgta cgttgcggat ccctattacc tgcccgccgt gcatatcctg
    18541 gagaagggga gcggccagga attcaccggg gccacggcga atcggaccgg cgacctcacc
    18601 gtgaacgggg acggcgcttc catgctggcc tggcgatacg tgtccgggac acccgccgtg
    18661 tggcgcctcg attgccggac caccaacctg actgttgtgg atacgttcga cgggcatatc
    18721 agcggcaacc cgatggatta cccgaccctt gtatctccag acggcgcgtt cgtcatcaag
    18781 cactacacca aactggccta ttccaacctg gcctccggcg cggtcaggat cttctccgag
    18841 gccttatggt cgtggtcgcc ggatggccgc ctgctggccg cggggtccaa cgtgctggaa
    18901 gaagcttcgg gccaacgcgt gtacgacttg ccgttttaca ccggcctgca agtcttctcg
    18961 ggcgacggca gcgcgctggt gcgcattcat ccgacaaccc gccaatggag catggtctcc
    19021 gcggcccttt attcggctcc gccggcgccg ccggccgggc cgataccgac cgacaacgcg
    19081 gtcgtcaatc ccgcgcccga cacactgcac tggacggtcg cggagcgcac ggtgcgttac
    19141 cacgtgtatt tggatacgga tgccgcgacc gtgggcgccg ccgagcccgg ggggacggca
    19201 tacctgggcg ccaccaccaa tgccgagtgg agtctgaccc atgccctggc ctgcgcgacc
    19261 acgtactact ggcgggtgga cagcgtgggg gtgtacacca ccacgaccgg caccgtttgg
    19321 actttccacg tgccggccgg gaccctgtca cccatcctga tctcgacaaa tatccccgaa
    19381 ggattctcgc ccgcccccgg gatcttcgac gtcgccttgt gcgggccggc gctgggctgg
    19441 acggtggcct gcgattgcgc atggatgcaa atggcgccga cgtccggtgc gggcaacgac
    19501 acggtgacgg gaacctacga caccgccacg ctgtcgcccg gcgtctatac gggccgtatc
    19561 agcctggcca tgggccgcga cgtcttggcc gtacccttat cgctgaccgt caccaccaat
    19621 cccgcgcttc acccgatacc aggcggggtc ctctttcctt cctgggttca ggtgttcgac
    19681 ggcgaaagcc acgcggacga tcgcggcgct gccgcggcca tggacccctt ggacaacgtg
    19741 gtggtggcgg gggaagtccg cggatccggc gacggggacg acatgctcct gctgaagctc
    19801 gaccccaatg gccagacgct gtgggcgacg aactacgacg gcagcgggca cggcgatgac
    19861 gccgtcaagg acctgggcgc ggacggcctg ggcgaaatct acgcgctgct cgactccacc
    19921 ggaaccaacg gcaacatgga cttggtggtt tgtaagtaca gcgcagacgg gcagttcctc
    19981 tgggagtatc gccgcaacgg gaccgccaat acccatgact acgcgacggc tctcgctgtg
    20041 ccgggcaccg gcggcgttgt ggctgccggg atgaccgtcg acgccgcgac cagcaatgac
    20101 gtgttcctgg cccggttgaa tgccgacggc acgccggcgt gggtcacgac ctacaacggg
    20161 ccccatgaga catcgcgcga tgaatgcaat gacatgagca cggacgctgg cgggtccatt
    20221 tatctcacgg gcacgagcac gtatcccggg ccgccccagc aatggcgggt gttcaagtat
    20281 tccgccggcg gcacccagga gtggagccag ttctggtacg cgcaatccgg ggccgccatt
    20341 gccgtaaatg aagcgaccaa tgtctgggtg gtgtccggcg ggggactgct tcgcaacctc
    20401 gatccggcca ccggcgttcc ccgctgggag cggaccctga ccttcccgta catgacgggg
    20461 tttgcagcct atgcccttct gcccggcccc cggacgcaag tctacgtggc gggtcgcgct
    20521 tcggacggaa aataccgcta cgccgcggcc tgggtcggtt cgaccggaga ggtcctggca
    20581 accgcgacgg tggatcgcga cgagcacagc gcgggccaca agtatctgaa caccgccatt
    20641 ctcgatgcct tctggcaacc ctaccttacg ggaaagggcg gaggaaccct gcgcttgact
    20701 ccatcccttg gggagttggg gcgcgtgacg ggcagcccga cggagggcat cgccctcgcg
    20761 gccgacagca cgggggccgt gtacatggtg ggctcctcgg acaacttcac aaaccagaac
    20821 gatctggtcg tggtcaaact ggcgcccagt ccctccgggg attttgacgc cgaccagatt
    20881 cccaatgact gggaaatcaa gaccggcggc aatgcctatg acgccgtgcc cgggcaggat
    20941 ccggacgacg attcgatgac gacctgggaa gagtacctgg ctgatacgga tccgaccaac
    21001 gggacctccg cactcgccgc gcccgcgctg gtgacgcggc cggacgggtt cttataccgg
    21061 atcacgggaa cctcaacggg ccggttatat gatatcttct ggaaaaccaa cctgctcgac
    21121 gcggcaacgc cctggcagtt cagcggctac agcaggctgg gaaccggcgg tgtcctggag
    21181 atcgttacga acatgccgac cgtcgatgtt ctattcctgc gttcaggcgc acgcctgccc
    21241 tgaacaaggg gaggcggagt tcgccatcgt caggtcagag taagagccgg ccaccgatcc
    21301 ccccaaggtc cccgttgttg ttcggtacgt gcaggaggag ggcgcagccg gtcgcggcaa
    21361 acggacgaag accccaaatc ctttgacagc gcgcgcggga gggtggtaag catgtccggg
    21421 ttcgtgtttc gcggcgggac cgtccggagg ggcgcggcga aacggaaagc atttatggcc
    21481 aaagtcatcc tcgagcacgt gtgcaagacc tatcccggcg gcgtgaccgc cgtgaacgac
    21541 gcgaacctgg agatcgagga ccaggaattc gtcgtgctcg tcgggccgtc cggctgcggg
    21601 aaatccacca ccctgcgcat ggtcgccggc ctggaggaga tctcgaaagg cgcgatctac
    21661 atcgacggcc gcaaggtcaa cgacgtgccg cccaaggacc gcgacatcgc catggtcttc
    21721 cagaactacg ccctctaccc gcacatgacg gtctacaaga acatggcgtt cggcctcaag
    21781 ctgcggcggt acccccgcga tgaaatcgac cggcgcgtgc gcgaggcggc cgacatcctg
    21841 ggcatccagg atctgctgga acgcaagccc aaggcgctct ccggcggcca gcggcagcgc
    21901 gtcgcggtcg gccgcgcgat cgtgcgcaag ccgaaggcct tcctgttcga cgagcccctc
    21961 tcgaacctcg acgccaagat gcgggtccag atgcgcgcgg agatcagcaa gctgcacacg
    22021 cggctggcct cgacgatgat ttacgtgacg cacgaccagg tcgaggcgat gaccatgggc
    22081 gaccggatcg tggtgatgaa ggacggcctg atccagcagg tcgcgacgcc cctggaactg
    22141 tataaccggc ccgcgaacca gttcgtcgcc ggattcatcg ggtcgccgcc catgaacttt
    22201 ttcccgggca cgctggaggc gcgcgccggc ggcctgtttt tccaggaggg ctcgttctcc
    22261 gtgcggacca cggacgaaca ggcgtccgcc ctgtcggcgc gggccggcca gccggttgtt
    22321 ttcggcatcc gcccggagga cgtggcggag gccacgggcg ccgttccggc cggggaacgg
    22381 atcgcggccc gcgtcgaggt cgtggagccc atgggcgccg aggtgtacgt ctacctgtcc
    22441 accgggaagc actccttcgt cgcccgggtc gggccgcaaa gcgcggtcga ggtgaaccag
    22501 acggtgccgc tgaccctgaa catgaagcag gcccatttct tccatccgga aaccggggac
    22561 gccctcgcgt aggccccggt cggaaacggc cgcgggccac aaggcataag gacgtggaaa
    22621 ttctcacggg catagcgcag tttctgctgg tgacggccat cgccttcggg gcgttcgtgc
    22681 taatcgccag ccacatgcgc ttccagcggc tgacccgggc cgccgcggag ccggacgctc
    22741 ccgatgctcc cgctgccgat cccctgaccg ccgaggtggc ccaccgtctg ggcaccgcgc
    22801 accgggagcc ggaaccgttc gcggtgatcc tcttcgagcc ggcggcggcc gccggccagg
    22861 cccttcccga cgacctggag ccgctgctcc caccgctgga ggccacgctc cggggatcca
    22921 tccgagcggg cgatggagtc ctgcgccacg gcggccggca gatcggcgcg ctgctctgga
    22981 tcacccggga ccgggtcgag tcggtggccc agcgcctgct cgaacggatc cgccgccccg
    23041 gcgaaccggg aggacgctcc ctcccgctgg ggattcacct ggcgaccggc ctggccacgc
    23101 accccgagaa cggaagccgg acgcacgccc ttctccaggc ggcccacgca gcgctggaca
    23161 gcgcgcgggc cgccggaggc tgggccgccc gtcttgccgc atcggacttg cccgccgtcc
    23221 cgccccctgc gcccgccgtc ccgcccaagg ggattctcga cgaactgacc ggcgtcctga
    23281 aggaggatcg actgatcacc gcgctgcagc gctcgctgtc gcgccatcgc aaggaggacc
    23341 gtccgctctc cctgctgcac ctggacctcg accacctgga tcgttacaac gagcactacg
    23401 gccgcgagac cggcgacacg ttgctgaagg gcatggccgc cgtgctgcag aagcacgcgc
    23461 gggaggcgga cctcatcggg cgactggagg gcgacagttt cctcctcatc atggattgtc
    23521 cgccgagccg cgccgtgccg gccacgcagc ggctgctggg cgaactccgc aaaacgccct
    23581 tcgcggtgcc cggcggcacg ctccgcgtca gcgcgagcgt cggcgtggcc gggtacccgg
    23641 atcacgccgc ccacccccgc gacctcgccc gggccgcccg gtacgcgctg cgcgccgcca
    23701 agcggcgggg cggcggtcga cccgtccttt tcgagccggc gatgcagacg atcgcagagg
    23761 agaatccccg ggaggtggac tcgttgtgag cgccgccgtt cctttccgcc cgctgcggct
    23821 gggcgtcaac ctcgaccacg tggccacgtt gaggcaggtc cggggcacgg agtatcccga
    23881 cgtcctcgat gcggcgcggc tctgcctcgg cgccggggcc cacgggatca ccgttcacct
    23941 gcgggaggac cggcgccaca tccaggacgc cgacgtcttc cggctcaagg ctgaactcga
    24001 cgccccgctg aacctcgaga tggccaacgc gccggggatc gtgcaggtcg cgctccaggt
    24061 gcggccggcg gaagtgtgcc tcgtgcccga gcggcggcag gaactgacca cggaaggcgg
    24121 gcttgacgtc gcgagacagg agaccgccct gcgcccgacg atcgaggcgc tgtccgacgc
    24181 cggcatcgag gtgagcctgt tcatcgaggc cgacgcgcgc cagctcgagg cggcggcgcg
    24241 gctcggggcg cccgtggtcg aattgcatac cggggcgtac tgcgatgccg ccggcgagcg
    24301 ggaagccgcg gccgagttga aacgcctgat cgagggcgcc cgccgggccc acgcgctggg
    24361 cctgcaggtc aacgccgggc acggcctgaa cacggacaac ctgcccccgg tcctccgcgt
    24421 cccgcacctg gacacgctga atatcgggca cagcctcgta gcccggtcgg tctttgtcgg
    24481 gctcgactcc gccgtccgcg aaatgctgga tgccatggcg ctgtacgagg gaggcgagcc
    24541 gtgaggcgga ccacggcgcg cgccggcgca ccggtcctgg gcgtcggcgt ggacctcgtg
    24601 gaaaacgagc gcatgcagtc cgccctccgg cgctggggcc ggacgttccg ggaaaaagtg
    24661 ttccggcccg cggagcagtc ctactgcgac ggaaaggcgg agccgtggcg ccactacgcg
    24721 ggacggttcg ccatcaagga agccgtcagc aaggccttcg gcaccgggat cggcgagcac
    24781 ctgggctggc tggacatcga ggtccggcgc gatcccgcca gcggggcccc ctcggtgcag
    24841 ttgagcgcca agggcggccg cctggcccgg cggcgcgggg tcggccgggt gctcgtcagc
    24901 ctttcgcata cccggcacta cgccgtggcc caagccctcc tggtcggcgg cgcccggaag
    24961 gagaagaccc gatgaagatc gtgacggccg aacagatgcg cgaactcgac cgccggacca
    25021 tcgaggaggc ggagaccccg ggcgaggttc tgatggagcg cgccgggcag ggcgtggcgg
    25081 acgccgtggc gcggctcgcg gagaccgccg ggttccacca cccgctcgtg cacctcatcg
    25141 ccgggcgcgg caacaacggc ggcgacgcct ttgcggcggc gcgctttctg aaggaggacg
    25201 gctttgccgt ccaggtctgg atcgcgggcg cggccaacga gatccagggc gacgccctcc
    25261 ggcacttcag ccggatgaag gacgcgaaga tcacggcgcg cgaattgccg acgcgggagg
    25321 actgggagga gtccctggcc tacccggcgg cgggcgacct tctcgtggac ggcgtcctcg
    25381 gcacgggcgg ttcgggtccg gcccggggcc cggccgccgg cgcgatcgag tatatcaacc
    25441 agcagtcccg ccaggcgctg gtggtggcca tcgaccttcc gtcggggctg aacgcggaca
    25501 ccggggccgc ggaaggcgcg gccgttcggg ccgacctgac ggtgaccatg gggctgccca
    25561 agcgcggcct cctggagccg gcggccctgg agtacgtggg cgcgctcgag gtcgtggaca
    25621 tcgggatccc ggcggaatac atcacccagg cggcgctcgc ggaggatcgc gagttcattc
    25681 atcccaacga cctgcggccg ctgctgccgc gccgcccgcg ggccacgcac aagggcagcc
    25741 agggccatgt cctgctgatc ggcggggcgc gcgggtacgc gggcgccatc gccatggccg
    25801 cgcgcgccgc gctgcggtca ggcgtcggcc tggtcaccgt cctgaccccg gccggcgtcg
    25861 cgcccatcgt ggccggctcg gtcctggagg ccatggtcca gggcgggccg gagacggata
    25921 cgggttcgtt gagcgcggcg ttctgggccg actggcggaa ccgcatcgac aagttcggcg
    25981 cggtgctgat cgggcccggg ctgacccgca accccgaggg cacggcgatc atgcggcacc
    26041 tgattcgcga atgcccgcct cccctggtga tggacgcgga cgccttgagc ctgctcgagg
    26101 ggcagcccca tttcatcgcg aaatgccgcc gcacggcgct gatcacgccg catcccggcg
    26161 aactggcccg gctgatggcg caggacatcg agacgatcca gcaggaccgc catgcggccg
    26221 cgcgggccgc cgccgagagc accggcgcag tggtcgtcct taaaggcgcg gggacgcttg
    26281 tcgccgagac tgggaagccg gtccagatga acctgacggg gaatcccggc atggcgacag
    26341 gcggcacggg cgatgtcctg gccggcctgg ccgccggact tctcgcccag ggcctgacgc
    26401 cgtacgacgc cgcgcgcctg gccgtctacc tgcatggccg cgcgggggac acggccgcgc
    26461 tgcacaagtc ccaggccggc ctgaccgcgc tcgacgtgct ggacgaactg ccctacgcct
    26521 tccgggagat cagcatccgc tgaacccggg cgggattgac gaggcgcgat ttggccggat
    26581 actggggccg acacccaccg aggacgccac gatgcaaagg acgatgctga agtcaaagat
    26641 tcaccgggcg acgctgaccg gcgccgacct ccactacgag ggcagcatcg cgatggatcg
    26701 ccggctgctc gaggccgccg acatcctgcc cggcgaacag gtgcacgtcc tgaacctgaa
    26761 caatggcgcc cgcctggtca cctatgccat cgcggccccg cggggctcgg gcaggatgct
    26821 gctcaacggg cccgccgccc ggctcggcct ggccggggat cccgtgacga tcctggccta
    26881 cgcgaacgtg gaggacgccg aggcgcgccg acggaagccc cggatcgtgt tcgtggacgg
    26941 gaagaaccgc atccggaagc cccgctgacg atcggctcaa ggcagggact ggccgaggcg
    27001 aaggtacttt ttctcgcctt ggaattcgag ccggacgccc ttggcgtcga cctcaacgag
    27061 ccggacttcg tcgatccggt cgcccacctc gacgaacgag ccgttcagaa acgcggagcc
    27121 ctttgatttc tcgccgtgcc cgaccatgac gccggtcagc ttcagcgtcg gccagcgctt
    27181 cgccttcgcc tcttccttat ccttcggctt ctcggccggc tttagctcgc tcggcggcgc
    27241 cgcgggggcg gcggcgggcg ccttttcagc gacgaccggc ggcggcgcct ccatcgctgg
    27301 ctccttctcc accggggccg gcgcgacggt ttcaatcggc ggcggcggcg gctcgagcgc
    27361 cggcggcgcg gcggcttcgg ccggggtctt cggagcggac ggcgccgggg cggcctcatc
    27421 ctgacgcggc accgccggcg cgaggggcgc aggtttcgga ccggattccg gcttcgagcg
    27481 gatgaaaaag gagggattca agtaccacaa cgcgaccccc gacgccgcga acagcaggat
    27541 caggagaact acccacaacg cgggatggcc ccggcccggg cgcggcggcg gggccggagg
    27601 aggcggcggc ccgtcttccg acgcttttcg atgcaaggct tcctgaatca ggctcacgag
    27661 gcttcctcca actccgacag ggcccttttc acttctcggt ttccaatctg ccggaccccg
    27721 gcaatgtaac cggccagcat cgcccggtca cagaccgtgt tcgtgatccg cgggatgccg
    27781 ccggcatagc ggtgcacgcg ccgcacggca tcgggctcga agcccgtccc ctcgccccgc
    27841 ccggccaccg ccagccggtg ctgaatatac aggccggtct cctcctcgga aagcggaagg
    27901 agatggcacg agatcatgat gcgctgccgc aactggcgga actcggggcg ggcgaggcgg
    27961 gcctggagtt ccggttggcc ggcgagcacg atctgcatca gcttgtgctg gtcggtttcc
    28021 aggttcgaaa gcaggcggac ctgctccatc acctcgggca gcaggtcctg cgcctcgtcg
    28081 atgaacaggg cgatgttgaa gccctgccgc gtctgctcca ggagaaattc gttgagctgc
    28141 tcgatgtagg ccaggcgatc gcgccctttc ggaaccaggc cgaaatcgtg gaggatggcc
    28201 cggagcaact gcgtctccgt caggcaggga ttgaggacca gggcggtgcg gacctgcttc
    28261 gagagcgcgg ccaggacggc ccggcacacg gtggtcttgc ccgagccgac ctcgccggtc
    28321 agggcaatga agcccttgcg ctgctcgatg ccgtagagga ggtgatcgta ggcctcgcga
    28381 tgccgggcgg agaagtaaag gaagcgcgga tcgggcgtga tgttgaaggg cgcctctttc
    28441 aatccataga aatcaagata catggcgccg cgctactcgt ggggttcctg ctcctgctca
    28501 tgttcgtgct cctgctcttc ccggtcgcaa gcatgctcga ccggaaagag ccggtccagg
    28561 gcatcgcgcg ggagggccgt gtccgccgag ccggaccgga gcagcgcgag atgatgccgg
    28621 acgccctcgt tcgccggcat gagttggagg gcctcctcga aatcccggat agcgggcgcc
    28681 cgggcgcccc gcagttcgcc gaggatcgcg cggaagctca agagccgggc ccgctccagc
    28741 ttcgactcct cgtcgcccgc cggcagcggg ttcggccatg cgcggagcgc ggcgtcggtc
    28801 aggcgcgccg cctcgccgtc ccggcgctgc cggaaggcaa ggcggccgcg ctcgccgagg
    28861 agccggaaat cgcggggatg aagccgctgg gcctcgcgca gcacctgctc cgccgcgtcg
    28921 agccggtcga gccgcgtgag ccagtaggcc atggtgagga aggcctccac gttacccggg
    28981 tcggcggcgg tggccatccg gagccagggc aggatctcgc tgacgctgta gccctcggta
    29041 tggatgtggc cggaggggcg gacggcggcg ctccatcgct ggaacaggct gttgatgaat
    29101 accacgtcat gcttgtggcc gacgccccgg tgaaagtagt cgtccgcccc cacgtagaag
    29161 ctctggccga acgccacgcg gctgacgccg agcgcgcggt cgagcgccga gtctcccgcc
    29221 ccgggatcgg cggcgcggcg cggcgccagg cggcaggcca ggctgaaggc caggatccaa
    29281 agcaggacca gcaccaggac gaggcgccgg ggtctcattc gggcgccccc cggcggaacc
    29341 gtttcgtccg gtaggccagc caggccagca ggaggaagag gcccgtccac accgcgccgt
    29401 agaccgcgat cccgacgaca tcggcccagg gcgcgggccc ccagtcgtgg atcagccgct
    29461 gccggagatc gaacaggtcc aggtgcggca gcgcgtagta gagcgccagc agcacgttgc
    29521 cggagggccc gggctcgtgg accagcagct ccggcacggc gggcgcgacg aaaaaggcgg
    29581 cgccgcacag gacataggtc atggaggccg ccgcgccgta ggtcatacgc gccgacagcg
    29641 ccagggcgac ggcgctgacc agggccagcg ccatcccgtg caggaggaac gcctggccca
    29701 ggcacggtcc gttgaccgcg ccgccgcgca gcgccgtcag cgcgacaatg accgcgtaga
    29761 aggccagcgt ggcgaaaacg gaggccgtgg cgctgcccag ccacttgccc agcagcagtt
    29821 cggcccgcgt cagcggcttg gccagcaggg ggaagatggt tccgcgcgac tcctcccgcg
    29881 gaagctgccg cgcggtcagg ctgacgacga ggaccagcga aaacagccag gccatcagca
    29941 ggccgacgtc caaaatataa cggaccaccg cgcccagccc gaacaggttg agggacatca
    30001 ggacgaacag cagggtcagc agcaggatcg ccatcacgta gagatccttg cggcgcagca
    30061 ggtccagcca gaccacgcgg gccatgacga ggatgcgctt catgccgatc cggccccctg
    30121 caccacgcgc aggaaatagg cttccagccg ttcgccggcg cggaccaatt cgtccacgcg
    30181 cccctccgcc accaggcggc cccgggccag gatggccacc cggtcgcaga ccaactccac
    30241 ctccgacagc tcgtgggagg aaaagaacac cgtcttcccc tgctcgcgca gccgggcgat
    30301 cagcccgcgc atctccaggc ggccgatcgg gtccaggccc cccgtcggct cgtccaggat
    30361 caccaggtcg ggatcgttga tcagcgcctg ggccaggccg atccgctgca tcatgccgcg
    30421 ggaatacgcg ccgatccgcc gccgcgccga ggccccgtcc agccccacct gctccagcag
    30481 ggcgtccagc cggcggcgca actccgggcc gcgcaggccg aacagtccgc cggccatcgc
    30541 gagcaactcg cggccggtca ggaaggggta caactggggg tgctcgggca ggtacccgat
    30601 gcggcggcgg gcgatggacc taccgacgtc ctcgccgaac aggaacgccg aacccgcgga
    30661 gggctggatg aacccgagga gcacgtgcag ggcggttgtc ttgcccgcgc cgttggggcc
    30721 gagaaagccc acgacggtcc cctgctccac cgacaggttc agcccgccca gcgcctcgac
    30781 gcgctcgccc ttgtcgcgat actcgacgcg cagatccctt atttcgatgg ccgcggccat
    30841 atgacgccgc ttcactatgg cccaaaacgc gcggcggaac aagttccgcc cgacccgcgg
    30901 ccgccaataa atccagttga cgtcgcgcct ttcccagtcc acgttttcca gacttggaac
    30961 gtcgggttca atcatgccgg ccaaacggat cataccgtgc ctggacatca acgccgggcg
    31021 cgtggtcaag ggcgtgcgct tcgtggtgct ccgcgacgcg ggcgacccgg tgcaggtcgc
    31081 gcgcgcctac gaggcgcagg gcgcggacga gctgaccttt ctcgacatca ccgcctcgca
    31141 cgaggcgcgg gacacgatcg tcgaggtcgt ccgcgccgtc gcgcagcagg tcttcatgcc
    31201 gctgacggtc ggcggcgggg tgcgcaccgc cgcggacatc cgccgcatgc tccaggccgg
    31261 cgcggacaag gtctcgctga acacggcggc gctgctgaac ccggggatca tcgacgagac
    31321 ggcgaaatcg ttcggcagcc agtgcatcgt cctcgccatc gacgcgcgga ggcggaccga
    31381 cggcccgggc tggacggtct tcacgcacgg cggccgcaag ccgacggagc gcgaggcggt
    31441 cgagtgggcg cgcgagggcg tcggccgcgg cgccggggag atcctgctga ccagcatgga
    31501 ccgcgacggc acgcaggacg gctacgacct ggacctgacg cgggcggtgg cggaggccgt
    31561 cccggtgccc gtgatcgcct ccggcggcgt gggcgcgctg gatcatttct acgacgggat
    31621 cgtgcggggc aaggccgacg cggtgctcgc ggcctcggtc ttccatttcg ggacgttcac
    31681 catcccgcag gtgaaggagc acttgaagaa caagggcgtg gaggtgcggc tgtgaacgag
    31741 aaagaagagg ggctggagct gcggctggat ttcaacaagc tgacgcaggc gacggcggcc
    31801 aacccgggaa tcatcccggt ggccgtgcag cacgcggaca cgaacgagat gatcctcgtg
    31861 gcctacacca acgagcaggc cttccggaag gcggtgaccg accggtcgct ggtcctgtgg
    31921 agcacctcgc gcaaggagtt gtgggaaaag ggcaagacgt cgggcgagac gttcgacctg
    31981 atcgaggtcc gggtcaactg cgagcagaac tcgctgctct accgcgtccg cccgcgccgc
    32041 ggcggcatct gccacacgaa aaacaggagc gggcagccgc ggaactgcta ctatcggcgg
    32101 ctgaacatgg agacggggaa gctggagaac ctggacccct gaaaaagaaa ccgcggatca
    32161 cgcggatgta cgcggatgag gaatcgaatg tgagctgttt tccggacagg aaggcgttcc
    32221 tggagaaggc aaagcagggc aacctggtgc cggtctggcg cgagctgctg gccgaccagg
    32281 attcgccggt ctcggcgtac gagaaagtgc gcgaccacct gcgccggcag ggccacgcga
    32341 cgcacacgtt cctgctggaa agcgtggagg gcggcgagaa cgtcggccgc tactcgttca
    32401 tcggcggaac gccgcgcgcg atcctccggg cctacggccg gcgcgtgacg ctgcaggagc
    32461 ccggcggcgc cgaggagacg ctggaagccg acgccgatcc cctcgacgcg ctccgccgtt
    32521 acatgacgcc cttccgcccg gtcgcggacc ccgccctgcc ccgcttcatc ggcggcgccg
    32581 tcgggttcct cggctacgac tgcgtctcgg tcttcgagaa gcgcgtcccc acccggaccg
    32641 accgggccct ccgcgcgccg gacatggtct tcctggtcac ggaggccctc atcatcttcg
    32701 accgcgtgcg ccacacgatc cggctgatcg ccaacgcctg gatcggggac gacgcggcgg
    32761 aggcgtacga ccgggccgtc gccacgatcg acgagctgac gggggccctg cgcgccgcgc
    32821 gcccgagcag cctgatcgac cggcacgcgc cggccgcgga gatcgagccg cggtcgaaca
    32881 tgacgcgggg cgggttcgcg gacgccgtgc gccgcgccca ggagtacatc cgggcgggcg
    32941 acatcatcca ggtcgtgctc tcccagcggt tcgagaccga cttccagggc gattcgctcg
    33001 acgtctaccg ggcgttgcgc gcgatcaatc cctcgccgta catgttctgc ctggacctcg
    33061 gcgacagcgc gatggtgggc tcctcgccgg aggtccacgt gcgctgcgag gacgggcgcg
    33121 ccgaggtgcg gccgatcgcg ggcacgcggc cgcgctcggc ggacccggcc gaggacgcgc
    33181 ggctcgccgc cgagctgctc gccgacccca aggagcgggc ggagcacatc atgctggtgg
    33241 acctcgcccg caacgacctc gggcgcgtct gccggtacgg ttcggtgcac gtgccggagc
    33301 tgatggtgat cgagcgatac agccatgtca tgcatattgt ctccgacgtg gcgggcgagc
    33361 tggcgccggg gatggacccg ttccagctca tgcgggcgac cttcccggcc gggacggtca
    33421 gcggcgcccc gaagatccgc gcgatggaaa tcatcgccga gctggagcag gcccggcgcg
    33481 ggccctacgc cggcgcggtg ggctacttca gcttcggcgg caacctcgac agctgcatca
    33541 cgatacgcac gatcatcctc gacctgggca aggcctacgt gcaggccggc gcgggcatcg
    33601 tcgcggattc cgacccggac cgcgagtacg aggagacccg gaacaaggcg cgcggcatgt
    33661 tcaaggcgct ggcgctggcc cggcagttcg cggccgcgcg ggagggcggc gcgccatgat
    33721 cctggtgatc gataactacg attccttcac ctacaacctc gtccagtacc tgggcgagct
    33781 cggggccgac gtgcgcgtgg tgcgcaacga cgccgcgacg gtcgacgagg tcgcggcgat
    33841 gggggccgag cgcatcgtga tcagcccggg cccctgcacg ccgaaggagg cgggcatctc
    33901 gtgcgcggtg atcgagcggc tgggcggcaa ggcgccgctg ctcggcgtct gcctcgggca
    33961 ccagtgcatc ggccacgtgt acggcgggcg cgtagtgcgg gccggccggc tgatgcacgg
    34021 caagacctcg ccgatccgcc accgcggaca gggcgtgctc gccgggatgc ccgagccctt
    34081 cgaggccatc cgctaccact cgctgctggt cgagcgcgcc agcctcccgg ccgacctgga
    34141 gatcacggcc gagacggacg agggggagat catgggcctc cagcaccggc gtctgccggt
    34201 gttcggcgtg cagttccacc cggaatcgat cctgacgcag aacggcatgc agatcctgcg
    34261 gaatttcctg gccgtcccgg ccggcttgta agggcgggga aaaccgcgta cagtgacggg
    34321 tgacgcaagc cgacggagca gggcgaccat ggacgaaaac cgagacatct accagtcccg
    34381 cggggaggcc aatccgccga ccggaccgtc ccgtcccatg gacatgggct tggacgggct
    34441 ccgacgccgg acgcgggcca ccacgatcct gctggccctt ttcatgctcg gcctgttgag
    34501 cgcggccatc tggctgatcc gcgcgcagga gaagaaactg gagcgcgagg aatcgggcga
    34561 gggcctcccg gccctcgacg tgagcccggt gccgctgccc gccgcgagcc agctcggccc
    34621 atcctccacg ggccagctgc ccgccgcgga ctccgcgctg cccctggagc cgcccacccc
    34681 gcccgagccc cggaccaacg ccccggcgct gacccccgag caggcggccc aggcgatgac
    34741 ggaactgcgg acgggccacg aatacctgcg ggcgcgcgac ctggaccggg cggaggagca
    34801 cacgcggcgg gcgctggcga tctggccgga gtggacatcc gcgctccgcg tgctcggcct
    34861 gatctacacg cagcgcgggc agttcgagca ggccatctcg gtgctggacc aggccatcgc
    34921 gcaggacccg atgagcgccg aggcggtgaa ctccctggcc acggcctaca tgcagaaagg
    34981 ctggatgggc cgggcggagg aactgctgca caaagtcgag atcatgaacc ccaactactg
    35041 gcccacgcac ctgaacctcg gcctgctgta catcctgaag aaggagtacg ggccggccgt
    35101 ggaatacctc gagcgcgccg tcgaacaggt gccgaaggac gccgggccgc gcaacaacct
    35161 gggcgtcgcc cagatgcgca tcggccgctt cgaggacgcg cgccggaatt tccaggtcat
    35221 catcgagcaa tccccggaca accccgcgcc ctacttcaac atcgccatca gctacgtcct
    35281 cgagaagaaa ctggacgaat ccctggagtg gatccgcaag ggggcagcgc attgctcgcc
    35341 catgacgtgc cagagttacc tggccgacca ggcgttcaac tccctgcgca attacgggcc
    35401 gttccagcaa ctgatgccca ccctgtaccc gcagctgccc gcccccccgt ccccatgagc
    35461 cggagtccgc accgatgaat tctgacgcgc agcgggagaa cgcgatccat gacctgggat
    35521 tcgcccaggt ggacctcgac cggccccgcc gccggggcat gcccgaggtg atcttctgcg
    35581 ccggcaagac gccggaccag atcatcggca tcatcggggc catgaagcag gccgggcaga
    35641 acgtgctcgg cacccggatg tcccccgagc agttccaggc catctccagg gccatcccgg
    35701 aagcggccta ccacgaaacc gcccgctgcc tgaccctcga agtcgcgccc cgcccgcccg
    35761 ccgccgggca cgtcgccgtc gtctgcgcgg ggacgtcgga cctgcccgtc gcggaggagg
    35821 cggccctgac ggcggagtgg atgggcgccc gggtggaagt gatccgcgat gtcggggtgg
    35881 ccggcctgca ccggttgttc aagcatctcg acacactgcg ccgggcgcgc gccgtcgtgg
    35941 tcgtcgccgg catggagggc gccctgccct ccgtcatcgg cggcctgatc gaccggccga
    36001 tcatcgccgt cccgaccagc gtgggctacg gcacgcactt cgccggcctg gccccgttgc
    36061 tggcgatgct caactcgtgc gcgcccggga tcaccgtggt gaacatcgac aacggcttcg
    36121 gcgccggggc cgcggccgcg ctcatcaacc ggatcgggga gccggcatga acctgctgca
    36181 tttcgacagc ctcggcgggg cttccggcga catgctgctc ggggcgctcg tcgacctcgg
    36241 gatcgaggcc gcggcgctcc agcgcgaact cgcgacgctg cccatcgggc ctttcagcat
    36301 cgaggccccg aaagatgcgg aacgccacat ctccggcacg cggctttcgg tccatatccc
    36361 ggagcacgac catgccgggc atcacgggcg gcatctcggc gagatccgca cgatcatcga
    36421 ggaagccgcc ctgcccgatc ccgtgaaggc catgagcctg aaggcattcg gacggctggc
    36481 cgaggccgag gcgcgggtgc acggaacggg catcgaggag gtgcacttcc acgaggtcgg
    36541 cgccgtcgac gccatcgtgg acatcgtcgg caattgcctg gcgctgcact ggctgaagat
    36601 cgaggccgtt tcgttcagcc cgctgccgct cggacacggg atgatccgct gcgcgcacgg
    36661 gaccttcccc tcgcccgcgc cggcgacggc ggaactgctc aagggctttc ccgtggagta
    36721 cgtggacgag ccgcacgaat tggtcacgcc gacgggcgcg acgctcctga cggcgtggcg
    36781 gtccgtcgaa cggccgccgg ccggcagccg cctcctgcgg aacgggtacg gcttcggcca
    36841 ccggaagctc gacgggcggc cgaacctgat ccgggcaacg ctcctcgagg cgcccgcggc
    36901 gggggccgtc ggcgagacct gcctcgtgct ggagtgcaac gtggacgaca ccacgccgga
    36961 gttgctcggc gcgctgtcca gccggcttct cgaggcgggc gccctggacg ttttcaccgt
    37021 cgcggcccag atgaagaaac agcggccggg cgtgctgctg acgacgctct gcctccccgg
    37081 cgaccgcgag cggctgctgg acctggtctt ccgcggctgc acgacgttcg gcgtgcgaga
    37141 atacctggcc gcccgcacca tgctggagcg ccggttcgtc gaggcgcaga cggattacgg
    37201 cccggtgaag atcaagatcg ggcgctggcg cggcgaggac atcacgttcg cgccggagat
    37261 ggaggactgc ctgcgcctgg cccgcgaacg gaacgtctcg gtccggaccg tctacgaggc
    37321 cgcctcccgg gcgcggcccc tggcctgacg tcagacgctg gagccgcgcc agttcagctt
    37381 gtggcggagc acggagaaat agctgtagtc ggggagctga accagccgca cgtcgcgcgc
    37441 gctgcgccgg atttccacgc ggtcgcccgg ctcgaccgac ggctcctcct gcccgtccac
    37501 cgagaggatc aggttcttcg cggtccgggt cacctcgacc tccaggcgct tgcggtccga
    37561 cagcaccagc ggccggttgc tcaacgtgtg cgggcagatg gcgcacacga cgaagctggc
    37621 gcaatccggg tgcaggatgg gcccgccggc ggacagggcg tgggccgtgc tgccggtcgg
    37681 ggtggcgacg atgagcccgt cgcagtgata ggtcgccacc tgctcgccgt cgatccgcag
    37741 gttcaaggtc atcagccgcg acgaggcgcc ccagccgatg acgatgtcgt tgagggcgcg
    37801 gtagcgtccc agcgtccggc cgccgcgcac gaggcggcac tcggccacgg cgcgcttcga
    37861 aatcgagtag cggcccgtga cgaggctctt gagggcgcgg ccgaggtctt tcgccgcaac
    37921 gctggtcatg aagcccaacc ggccgaggtt gacgcccagc acggggacgg cgcgccccgc
    37981 gagcaggcgc gccccgcgca ggagcgtgcc gtcgccgcca agggcgacga gggcgtcgat
    38041 ccgtcccgcc agttcgcggg cggcgaccgc gcaacaccgg ggcagcatcg gggcggtctc
    38101 gtcgcaagca agcagcttga gccccatctt gccggcagag cccgccaagc gcttgagggc
    38161 cgccgcggcg cccggcttat ggcagttggc caacacccct atggtcttca tgggtttccc
    38221 ttctcccaca aggcgaggaa ctccacgttc ccggccgggc ccgtcaacgg ggacgggatc
    38281 cagcctttcc atgatagccc cagttccccc gttccaaaag cccgaatgcg ctcgatcacc
    38341 tcggcgtgga ccgccgggtc ccgcacgacc ccgccccgcc ccacctgctc gcgaccggcc
    38401 tcgaactgcg gcttgatcag ggtcacgatc ttcccgccgc cggccaggac ccggacgatg
    38461 gccgggagaa tcagcgtcaa cgagatgaac gccacgtcgg ccgtcgcaaa ggccggcggt
    38521 tcggagaaat tcgcgggata caggaaccgg gcgttcgttc cctccatcac gacgacccgc
    38581 ggatcctgcc gcagccgcca gtgcagctgg ccgtggccga cgtcgaccgc gtacaccttg
    38641 gccgcgccgc gctggagcag gcagtccgtg aacccgcccg tcgaggcgcc gacatccagg
    38701 cagacgagcc ccccgacatc cagccggaag tgcagaaagg cggcctccag tttctccccg
    38761 ccgcggctgg cgaaccgctc cggcgtcttc acggccaccg ccgccgcttc gcccacctcg
    38821 aaagcgggct tgtcgcggac ctggccgtcg acggtgactt cgccggccag gatcaggcgc
    38881 tgcgccttct cccggctttc ggccaggccg aggtccgtca tccgctggtc gagccggatt
    38941 tttttcatgc ggccggcacg gtcagaacat ccacacgggc ccgatgaggc ccgagaaatc
    39001 atccgaggtg gaatgctcca tgtagtccac gcgcgcgctg acgccccacg tccggttgac
    39061 catcgcgatc agccgggcgc ggagcacgtt gaagtcctcg tccgggtcca ggtcctggct
    39121 gtcctggtag tagtaattga cgcccgcctc cagcatccgc cagcgccagg ccgcggtcag
    39181 cccgtacagg aacgagtcgt tgtcctgctc gccgcccggg ctgtccaccc gttcccaacc
    39241 gtagccgagg tagggattca ggaacaggtg ccacgccttc acgcggaaca cggggccggc
    39301 cttgatcatc ggcacgttca ccttgatctc cgcgccctcc ggcttgatct cgtgccacag
    39361 gtagccgacg cccgcgttcc aggtcaccgc cgatttcgcg tcggcgtagt agttggcgaa
    39421 gaacaggtcg ccccacacgt cgcagtcgtt gacgtcggaa tagaagaaga agttgttgac
    39481 gacgacattc ggatgcagcg ccatgacgac caggccgtac tcgggcccgt tgtcggtcac
    39541 ggtcacgggg cccatcggcg tccgcatctt gagctcgttc cggtccgcgc cggccatggg
    39601 actgaccatc cagcgccagc ccggggcgtc ggccatcggc gccgtctccg cggctgcgga
    39661 caaggcggtc gccaacatcg cggccatcat cccgcattgc cttcttctca tggttcctcc
    39721 cgctcttccg cgtttctcac acggcataac tgtgcagacc gccgaacagc ctcgacaggt
    39781 tgacccacga cagcgtcagt ataatggcgg caaagcccgc caggagcaag acggcataga
    39841 ggcgctccga gccgcggccc aggaccttcc gcgcatggaa atatcccgcg tacagcagcc
    39901 atgtcgccag cgaccacagc tccttggggt cccagtgcca atagtcaccc cacgccagct
    39961 tgccccacca cgcccccagc agcaggccga gggtcagcag cggaaatccc cacgaggcga
    40021 gggcgtgggc cgaacggtcc gttgcgtcgc gctgttccgg cgaggcgccg gcaaactgga
    40081 gggccgacac cgccgcggcc ttggccagca gcaggtatgc gcccaggtag accagcacgt
    40141 gcggggcgaa aagagggctt tgcagggcgg gcggcaacgg gcgcagcgtc tcggccaata
    40201 cgaagcccgc cggccagagg accaggaacc ccagcagggg atccagccgc gcgcccggca
    40261 cgggaagccg gcgcgccggg agcgccagcg gccagaccag cgcgcccatg gcgagaaaca
    40321 cctcgaagag gttctgcagc ggccagcgct ccgcggacag tccgcgaaac aggaccgcgc
    40381 cgagcgccac ggcaaagccc gcgttgaaca ggcgtcggga ccagcggccc ccgcgccgcc
    40441 atcccgcggc aaacgccgcc gcatagaggc ccatggcggc gtagatcagg aggcccaaca
    40501 ggctggtttt tatgttcaca gcgcggcctc cgcgcgtcgt ggcggccgca tccagaaatg
    40561 ccaggccgcg ccggcgccca gcagcacgaa gcccatccat accgccgccc agccccggtc
    40621 gctggcgatc cgcagaaccg tgtacggctc cggctccgag ccccaggatt gctggtacac
    40681 gtgatagccc gctacatgca gcggatggtt gacccggacc tccgccggcc gggccttccc
    40741 gccccgcagg accgtcagcc ggctccggta ttcccggacc ggcgcttccg gccggggcag
    40801 gatcaggctg gccgaccgat ccagattggt caggtccgga aacacgggat acagcgccag
    40861 ccgcgcaaac ggctcgtccg gccggcacac cagcgtatgg acacgaggcc cgccgtccgc
    40921 cgataactcc acggtcagcg acaggggctc gttctccgcc ggccgggtga actccgtcac
    40981 gcgccattga atcggggcgc cggcgaggcg catggtcttt cccggtttcc actccagaac
    41041 gcgcgtccgc cagtggaaac ggggcgtgcc gggctcgggc tccagaaccc cgtacagcag
    41101 cgaaggcgga tcggacggca acgggtagcg ctcgatctcg aacgcgtcca gccgcagcgc
    41161 aaacggcaga gcgcccagaa cgcggccgga cgccgggtcg atcacgtcgg cggaggtttc
    41221 gccatcccgc aacgaaagga cgccgctgcg gacgcgcggc gggagcccgc atttttccgc
    41281 cagccggtgt ccggccccgg acccggtcat ggcgcccgcg aggatcaaca gggagccgag
    41341 gtgcatgccg agcaggcccg gccgccgcca cgcggccgga tacgccgcca ggccgacgac
    41401 gagggccagc gcgaaggcga tccagaacac caccatgggc ggggcgttga acagcctagt
    41461 cgccgcctcg gcaccgagga aggctccgaa aatgctcaag cctgccccgc cgaaaaggag
    41521 ggccagcgcg aaccactgga gcaagcggcg gaactcgctc atggacaatg aatacagcat
    41581 ttcggccgta tcggaaagtc ctttacactc acccgacccg cgcgccatac tgacgtcgtg
    41641 gcgatgatcc cttggagaca atgcctgctg ctcgaggggc tgcacccggg ttggcgcctc
    41701 gcggtcttcc tgatgggcgg ggtattggcc ggcgcggtca tcctggtcac ccacatctcg
    41761 cgcgcggctt cgtacttgag cgacgagccg gaagtctgca tcaactgcca cgtcatgaac
    41821 aacgcgtacg ccacctggcg gcacggcagc cacggccacg tcgcggcgtg cgtggactgt
    41881 cacctgcccc accagaaccc cgtcgccaag ctggcattca agggcatgga cggcatgcgc
    41941 cactcctatg tcttcacctt ccgcctcgag ccgcaggtgc tggagctgtc gcacggggcc
    42001 atcccggtga tccaggagaa ctgcctgcgc tgccaccatg accaactgca gatggtccgg
    42061 ttggcggcgg tcacggagcg gcgctgctgg aactgccacg acagcgtgca cggctcggtc
    42121 gtcagccttt ccgcctcgcc ggacgcccgt cggccggcgt tgccgtccgc cgggctcgaa
    42181 tggttcaaga agcaaacggg agacaagcca tgaacagcga atccgcgaaa tcccgactgc
    42241 cgctctgggt cggcccggcg ctattcctgc tgacgacggg cgtcgtcgtg ctgctcgggc
    42301 tgctggcctt ctccatcacg gagcggcgct gggagaccca gcggccggcg atcgcgatcc
    42361 agcccatcgc cgagtgggag cccgacaacg ccgcctgggg cgccaactac ccgcgcgaat
    42421 atgagcgcta cctcctcacg cgcgacgata ccacgcgcac gatgttcggc ggctcgttcc
    42481 cgcgcgacta cctcgaggac gatccgcgcc aggtcctgct cttcgccggc tacccgttca
    42541 gcaaggagta ccgtcaggcg cggggccatt accattccgt gaccgacgtg acccagacca
    42601 agcgcatcag cgcgaagaac atgtcgagct gctggacctg caaaagcacg gacgtgccga
    42661 agcgcatggc cgaaatgggc gtcgcggagt tttacgcgac gcccttctcc gagttcgtcg
    42721 ggaaaatcac ccaccccatc ggctgccagg attgccacga tccgaagacc atgaacctgc
    42781 gcattacccg gccggccctg cgcgaggcct tcgcggcaat gggcaaggac atcgaccagg
    42841 ccacccacca ggagatgcgc tccctggtct gcgcccagtg ccacgtggaa tactacttca
    42901 aggacggctc gtacgtgacg ctgccgtggg ccggcggcct cggggtggag cagatcatcg
    42961 cgtactacga cgcgttgtcc ttcaccgact ggacgcacgc catttccaaa acgtccatca
    43021 tcaagtccca gcacccggat tacgagctct actccgccgg catccacgcc tacgggggcg
    43081 tcgcgtgcgc cgactgccac atgccctacc ggaccgaggg cggggtgaag ttcaccgatc
    43141 accatgtcca gagcccgctg ctcaacatcg ccaatagctg cgccgtctgc caccgctggg
    43201 gcgaggaaga aatacggagc cgcgtcgagg ccatccagac caaggtggcg aacgccaagc
    43261 tcgcggccga ggacgtcctg gtgagggccc acttcgatat cgcggccgcg atgcaggcgg
    43321 gcgcgacgga cgaggagttg gcggccgcgc ggaagctcgt ccggcacgcg cagtaccgct
    43381 gggatttcgt gtcctcgcac aacggcgtcg gcttccacgc gccccaggaa tgcacgcgca
    43441 tcctcggcga ctcggcgcgg gaggcccagg aggcgcgcct gctcgtcgcc cgcctgctgg
    43501 ccgcgaaggg catcagcgcg gaaccgaaat acccggacct ctccacccgg gaccgcgccg
    43561 tggaggtgaa gaacgccttc gagtccgggc agccgacaaa tctgctgtag gcggccgccg
    43621 ccggggcgga ctaccggacc gcgaccacga ctttgaacca ccggaaggcg cgcaggtcgt
    43681 ggatcgtcac gcggcggaag cccgcccgaa ggcacagcgc cctccactgc ccggcgctga
    43741 cgcagtactc gccgaggttg aggatgtgtt ttttgccgtt gtcgtacatc cggctgaagg
    43801 ccggcacgcc gccctccttc agggcctcga cgatttcatc cttaaggaaa tccaggatcc
    43861 gcgccagccg tttcggatcg gcgtgatcgc ccgtcgtatc cacgtccact tccccgatga
    43921 ccagtcggcc gccgggcttg aggatcgagc gcatggtttt cagcatgggc cgcttgttct
    43981 cgacgtggtg gagcgcgacg gtggcggcga tgatgtcgaa cgaggacttt ccgaaccgca
    44041 gcttcgccgc gtcctgccgc gcgcaacgga cccggtcccg caggcccagc gcgtcgattt
    44101 tggcctggaa caaccgccgc atgtccggcg agttgtcgac ggccgtaacc cggcagtccg
    44161 cgcgcttcag gaacttgagc gacagcaggc ccgtaccgca gccgacgtcc aggacgcggt
    44221 cgccgtccgt cacccccgac aagcgggcgg ccaggtccag cagttggtga tggcgccgaa
    44281 ccttgcccag cgtggcatcg tattcgttcg cccagtcgcc gaaccagcgc gtggtgacgt
    44341 gatttttcat cagccggcca ttgaatgcgc gggcgctttc cttgtcaagc gccgcaggaa
    44401 agcctccgcg aatccgcgct tgctgccgcg cccctccctg tggtatccgt acgcgactcg
    44461 tacggccggc ccgtggagcc gccatgcacg accggatcac catattggat ttcgggtcgc
    44521 agtataccca gctgatcgcc cgccggatcc gcgagcagca ggtattctgc gagatccggc
    44581 gtttcgacac gccggccgcc gacctcgccg cgcgggaacc ccaggggatc atcctctcgg
    44641 gcggcccggc cagcgtcctc gtccccggcg cgccggccgc cgaccccgcg atcttcggcc
    44701 tgggcatccc cctgctcggg atctgctacg gcatgcagct catgggccgc gacctcggcg
    44761 gacaggtccg gcccggccgg gcccgggagt acggaagcgc cgtggtccgg gtgcttcagc
    44821 cggacgccct ctttcgcgaa ctgcccccta ccctcgacgt ctggatgagc cacggggacc
    44881 aggtggagca attgccgccg ggcttcgtcg cgctggcggc cacggacacc tgccccatcg
    44941 ccgccatggc cgacccggcc cgcgagctgt acgcgctgca gttccatccc gaggtcgccc
    45001 acacgccgcg cggcgcggac gtcctgcggc atttcctctt cgacgtctgc gcctgccgcg
    45061 gcgactggaa gatggcggat ttcgcggagg agagcgtcgc ccgcctccgc gcgcaggtcg
    45121 ggagcggcca ggtgttatgc ggcttgagcg gcggcgtgga ctcgtccgtc gccgccgccc
    45181 tgctccaccg cgccatcggc aaacagctcc attgcgtgtt cgtggacaac ggtcttctcc
    45241 gcgcgggcga ggcggagcag gtcgaggaga ccttcggccg cgccttcggc atggacctgc
    45301 gcgtcgctcg cgcgggcgac ctgttcctgg agcggctggc gggcgtcacc gacccggagc
    45361 ggaagcggaa gatcatcggc gcgacgttca tcgacgtgtt ttccgaggag gcgcgcaagc
    45421 tggatcccgt gcggtttctc gcccagggca cgctctatcc cgacgtgatc gagagcgtct
    45481 cggcgttcgg cgggccctcg gcgacgatca agagccacca caacgtcggc gggttgccgg
    45541 cggacctgaa gttcgagctg atcgagccgc tgcgggacct gttcaaggac gaggtgcgcc
    45601 agctcggccg ggagctgggc ctgccggatt ccatcgtcga gcgccagccg ttccccggac
    45661 ccggcctcgc cgtgcggatc ctcggcgcgg tctccgcgga gcgcgtcgcc ctcctccagc
    45721 aggccgagat ccggctccag gaggaactcc gcaggttgcc caattaccgg gagatctggc
    45781 agtcgttctg cgtcctgctg cccgtccaga cggtgggcgt gatgggcgac gagcggacct
    45841 acgaaaacgt cgtcgccgtc cgcgccgtgc agagcctgga cggcatgacg gccgactggt
    45901 tccggctgcc gcacgagacg ctcgaccggg tcgcctcgcg gatcatcaac gaggttcgcg
    45961 gcgtcaacag ggtggtatat gatataagtt cgaaaccgcc ggctacgatc gaatgggaat
    46021 aagaagttga accacagagg acacagaggg attttattca accataggga aacggggatt
    46081 atcatgtttc ctatcattgg aaaaatggcg gtgacttttt ccacacattg gaaaattccc
    46141 ggctttcctc tgcgttcctc tgtgccctct gtggtggata attgaaatgc caaaacgaac
    46201 ggacattaaa aaaatcatgc tcatcggctc gggcccgatc gtcatcggcc aggcctgcga
    46261 gttcgactac tccggcaccc aggcctgcaa ggcgctccgc gaggagggct acgagatcgt
    46321 cctgatcaac agcaacccgg cgacgatcat gacggacccc gcgacggcgg accggaccta
    46381 cgtcgagccc atcacgcccg aggccatcga gaagatcatc gccagggagc ggccggacgc
    46441 cctgctcccg acgctgggcg gccagacggg cctgaacgtc tccatcgaga tcgccgagcg
    46501 cggcatcctg gagaagtacg gcgtggagat gatcggcgcc aacctcgagg tgatccagcg
    46561 cgccgaggac cggcagctct tcaaggccgc catggccggc gccggcctgg agtgcctgaa
    46621 gagccggatg gtcacaacgc tggaggaggc cgaggccgcc gcgcgcgaga tcggcttccc
    46681 cgtcatcatc cggcccagct tcacgctcgg cgggaccggc ggcggcacgg cccgggacat
    46741 ggacgagctg cggcgcaccg cggccggcgg cctgaaggcc agcctgaagc acacggcgct
    46801 gatcgaggag tcggtctggg gctggaagga atacgagctc gaggtgatgc gcgaccgcaa
    46861 ggacaacgtg gtcatcatct gctccatcga gaacctcgac ccgatgggca ttcacacggg
    46921 cgacagcatc accgtcgcgc cggcccagac gctgacggac gccgagtacc aggagatgcg
    46981 cgacgcgacg atcaaggtca tgcgcgcgat cggcgtggag accggcggct cgaacgtcca
    47041 gttcgcggtc aacccgaaaa ccggccgcat ggcggtgatc gagatgaacc cgcgcgtgtc
    47101 ccgcagttcg gcgctggcga gcaaggccac cgggttcccc atcgccaaga tcgccgccaa
    47161 gctggccgtc ggctacacac tcgacgagtt gcccaacgac atcacgcgcg aaacgcccgc
    47221 gagcttcgag ccgaccctcg actactgcgt ggtcaaggtg ccgcgcttcg ccttcgagaa
    47281 attcgccggc gcggacccgc gcctcggcgt cagcatgaaa tccgtcggcg agaccatggc
    47341 catcggacgg aacttcaagg aggcgctgca gaaggcgttc cgcgggctgg agatcggcgt
    47401 ggacgggctg ggccgccgcg agttcgaccg gatcgaccct acccggctgg aggagggcct
    47461 cgccacgccg cacaacgagc gccacttcta catcaagtac gccctcgaac agggcatgcc
    47521 gatccgggat atcgcggcga agacgcacat cgacccgtgg ttcatcgacc agatgcagca
    47581 gatcgtcgag atcgagcgcc ggatcaagga cgtcccggcg ggccgtcccc tgccgatcga
    47641 gctgcatcgg gaggccaagg aaaacggctt ctccgacctg cagatcgccg ccctgcgcgg
    47701 cgaccggccg acgcagctcg aggcgcgcaa ggcccggacg gacgcgggga tcaagccggt
    47761 ctaccggctg gtcgacacgt gcgccgccga gttcgaggcc tacacgccgt acttttactc
    47821 cagctacggc ggcctgtgcg accagacccg ccggacggac cggaagaaag tcatggtcct
    47881 cggcggcggc ccaaaccgaa tcggccaggg catcgagttc gactactgct gcgtccacac
    47941 ggtcatggcc ctgcgcgagc tgggctacga gacgatcatg gtcaacagca accccgagac
    48001 cgtctcgacc gactacgaca cggcggacaa gctctacttc gagccgctga cattcgaaga
    48061 cgtgatgaac atctacgaga acgagcggcc gctgggcctg atcgtccaga tgggcggcca
    48121 gacgccgctg aacctcgcgc gctggctgac gcaggccggc gtgccgatcc tcggcacctc
    48181 gacggccagc atccaccagg ccgaggatcg cgagcaatgc cgcgtcctgc tcgatgagct
    48241 gggcctgaag cagccggaga gcggcacggg catgacggag gccgaggccc tcgcggtggc
    48301 cgagcggatc gggttcccgg tgatgatccg cccctcgttc gtcctcggcg ggcgggcgat
    48361 gatggtcgca cacaacaagg ccgacgccgg gccgttcatc caggccgcgt tcaaggccag
    48421 ccccggcttt ccggtgctcg tggaccgcta cctcgaccgc gccatcgagg tggacgtgga
    48481 cgtgctgtgc gacggcgagt cggtttatat cggcggcgtc atggagcacg tcgaactggc
    48541 cggcatccat tccggcgaca gcgcctgctg cacgccgccc cacacgctgc atccgtccat
    48601 ccagcggcgg atcgaggacg cctgccggcg catcgcgctg aagctcgaag ttcgcggcct
    48661 gatgaacgcg cagattgccg tgaagaacgg cgagatctac atcctcgaag tcaacccccg
    48721 cgcctcccgc acggtcccgt acgtcagcaa ggccaccggc gtgccgctgg cgaagatcgc
    48781 ggcgcggatc gcggtaggcc agaaactcaa ggatatgggc ttgggcgaac ggccgccgaa
    48841 agttccgtgg ttcgccgtca aggaggccgt gctgcctttc aaccggttcg ccggcgtgga
    48901 cccgatcctc ggccccgaga tgaaatccac cggcgaggtc atgggcatgg acttcagctt
    48961 cccgctggcc tactggaaga gccaggtcgc cgccggccag gccgtgccga gctccggcac
    49021 ggtggtgctg tgcgcggcgg acccggacaa gtcatggatc acggaaatcg ggtgggaatt
    49081 gcaggacctg ggcttcacgt tggccgccac ggccggcacg gccgcggcgc tcaaggcggc
    49141 cggcgtggaa agccgggtgc tgcaaaaggt gcgggagaat aaatcgccca acatcctgga
    49201 cctgatgagg gccaacgaga tcgtcctgct catcaacacg cccaccggga tcaaggaccg
    49261 cgtggacgag gtgcacatcc gctccgaggc gatcctccgc agcgtgccca tcgtcaccac
    49321 cgaggccggc gcgcgggcga ccatcgccgc gatacgccac atgcggggac aggcgtggga
    49381 cgtccacgcg ctgcaggatt ttcacccggc gaaggcttga cgtcgcgggg aagcgccgct
    49441 accggggcag cggcgcgggc tcgtcttcct tctcttcctc gtaggccacc agcatggcgt
    49501 gacggcggtt gaagaagggg ctcaagaagg cgaagtagaa tccgaaggca ttcagcgtga
    49561 aggccgcggc cacggcccag cgggagttga agaacagcgc ggtgatcagc aacacggccg
    49621 gcgccatgcc ccggagcccc ttggtcggct tgttgtagtg gacgtgcgac acctggagca
    49681 ggaccatcgc cagcacgcag ccccagatga aggtcgccgt cggcccgtgg ctcagggaga
    49741 acacgtcgtc gctgatgtag cccgattccg cgatcaggac cgagagcgcg atccagcccg
    49801 cgcaggtcgt gatcggcagg ccgaggtagc cgcgctggcc gcggaaggga tccaccacgc
    49861 ggaaccggga aagccgcgag acgccggaga ccacgatcgc gcaggtgatc acgagccccc
    49921 agatcccggc gtccttcagg accaactggt gcgccaggaa cgcgggggcc aggccgaagc
    49981 tggtcatatc tacgtacgtg tccagctcgc cgccgaattt cgtagtggcg
//
LOCUS       DAOOTK010000011        48893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_560_length_48893_cov_6.408739, whole genome shotgun sequence.
ACCESSION   DAOOTK010000011 DAOOTK010000000
VERSION     DAOOTK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 48893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48893
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_48893_cov_6.408739"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PK634_02610"
     CDS             complement(<1..1138)
                     /locus_tag="PK634_02610"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOW96660.1"
                     /translation="MRPCLLKALWLSPWSRHPAFLWRKIRSKLGAEPPLVVRPRPPGE
                     GRCAALPVDGLRRWFEACEWQAEPYPWTRVFHRDFSEEFLLGRCHSGGPLNPEDLASL
                     AAASGPNGARDLTGDIKLPWEFSRGYAFVLQAAGTPSGRRETAAAEIAEKIVLWEEHN
                     RDLGGPNWICAMEVALRAVNWACADALLEGGVSERVGRDRWLDGLWTAGQVVEQRIEA
                     RKVSSNHYLADLLGLAVIGLHFPATADGRRWRRFGVAEMQKALLRQTYRDGGLYESSL
                     PYHALVTEMALLLALFARESLRPAFLRRLERMARVVAACLDAGGDVFPIGDEDGGRVI
                     PLDFISRRGRGEILLQLAEQVFPGADFSRPSEAFFPRSGWWVRRR"
     gene            complement(1119..2423)
                     /locus_tag="PK634_02615"
     CDS             complement(1119..2423)
                     /locus_tag="PK634_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96661.1"
                     /translation="MKKVLVSAYFFMGGSSMGDIRVRRALRNLHRHGYKPVIVTHDRN
                     RDQWPASDDYELVPVRGLDLAKAYRRLRGREGSFAPAPAAGPKDLVSMRTGFSTWIKQ
                     WLLVPDPQMLWGPAALRAAREVLRRERVDAVYASLAPRTDMRVATRVAAEFGLPCVVE
                     YRDLWTSNFYDNLKAPTPLHRWLHARMERTAIRRATRITCVSTGLADYLRARYADLRP
                     EISVDYGFFDPAEYPPRPPREPGRPLTVSYVGKFYFSRQPGVFLEGLRRFVERTGIRP
                     GQFRFQWLGEIIGVDRLRETVEAMGLGPFVEYYGHVPHRQAIETLTRSDVALIVQAPQ
                     DTIHIPGKIYEAMGARTPILYISPPFEGTEIVERTGAGLCCPHDAEAVGETLARFRAH
                     REQGGPWPFREDEVRRFDKDVFLARLAVLFDQAIADHATLPA"
     gene            complement(2420..4594)
                     /locus_tag="PK634_02620"
     CDS             complement(2420..4594)
                     /locus_tag="PK634_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOW96662.1"
                     /translation="MKQVLQDLTQAAVTVVEVPVPEAPSGFVLVRNAASIISAGTESA
                     QLAAAQAGALQKVSERPELIGRAFRMLRDQGLSALMKKVGAKKTGYAPLGYSCAGVVE
                     RAGAGVDRFPPGAPVACAGVGYAVHAEYACVPQNLCAPVPEGVSLEAAAYTTLGAIAM
                     QGVRQAAVALGEGVTVIGLGLVGLLTVQLLKAAGCRVAGVDPAENARKWGRTAGCDET
                     ADPAGAGQAVLRMTRGLGADAVIIAAAASGSEPVQLAGRLARSRGRVVMVGSTGMDIP
                     RQLFFEKELSFCLSRSYGPGRYDPTYEEGGLDYPAEYVRFTEQRNMEAFLQLAQEGKI
                     RPENLTTHRFAFADALRAYDALKNRAEERVGIVLQYPEAPAPRAPRVDRPAAPRAAPG
                     ALGVGFIGAGSYARAMLLPVVSRNGNARLRGVADQRVDASAAAARDFGFAFAAAGAAE
                     VLGDADTQVVFITTRHDSHASLVLEGLKAGKQVWVEKPLALTVEELRAVAAAVRAGPE
                     ARLVVGFNRRFSPLTQFIRARLGEAGPVMIQCRVNAGPAPARHWTQDPKIGGGRLVGE
                     GCHFFDLACCLAGGRPVSVVTRALKGDRADLLPTANFAALVSFDNGSVASLAYSAQGS
                     PLLPKEHLEILAGPGVARLDDFRRAEWYGPARALREKLPQQDKGQAALVQAFLDSARR
                     GGPPPISFEDLLYSSALTLAAQQSLESGREARVRDVLDAAQA"
     gene            complement(4591..6087)
                     /locus_tag="PK634_02625"
     CDS             complement(4591..6087)
                     /locus_tag="PK634_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOW96663.1"
                     /translation="MPLVAVEQTTPSYPDEPPFHPGERYPEAPFPDTAGPNPVYAAVR
                     RLLMRLGMDEAHAGSPDWNPLGAIVRPGDTVVLKPNFVISRNLGGGDLFAVVTHPSVL
                     RALADYCFIALRGRGRLVIADAPEMGCDWDELMRALRLDAVADFYRRAAGFPLEICDL
                     RNFALVDNRKPAYAHNRKPLPGDPAGSTVINLGRESAFYGMDSARYYGADYDRRETIR
                     HHHGETHEYCISNTVLRADALISVPKLKTHKKVGVTLNLKGLVGINTNKNYLIHYRLG
                     TPRTGGDQAPDALAAGVRLKVRVQRWLFDRALARQTAWGDAVYAAARGVWRRLFRPFV
                     GATAGEPIVDAGNWHRNDSAWRMTSDMARILFFADRDGVLRDRVQRRMFCLVDGIVGG
                     EGDGPLAPTARPSGCLVGGENPLAVDLVASRLMGFDPARIRQFDVLKDRRRNFGFSAP
                     EEIRVAQGERVAPGAEYFSARDIDPRLNFQPHSEWKGHIEIASNAEAP"
     gene            complement(6075..7466)
                     /locus_tag="PK634_02630"
     CDS             complement(6075..7466)
                     /locus_tag="PK634_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96664.1"
                     /translation="MKGSPAVREFLSRLYSLVPARRRWPAAFWKWYAFYEDAETWPVE
                     RMEAYQLERIRRLLSSARAGSPFYAEKLKDVRPDDIRSLADFSAAVPATTRRELRENM
                     DRWPCRDRAAKAERGCTSGTTGMALQFLHPPGDRWREWASICHQWKRVGYDPAHSVRA
                     TFRGLTLDPAGVDRRPHSNTVRFAILGFDRARLERYAAEIRRCRAEFFHGYPSALSLL
                     ASTIRHEALDFPQPRAILLASEVVYDWQLAAIREAFPDARLFAHYGCAERAILAGWCE
                     RRPAYHVLSPYGFLEVSPDTGEVMATNLHNDVSPFIRYRMTDTVRGVSADACPDCRRP
                     YRPLVEELVGRQEDYVYSPARGWIGPAIITYPFKGLKAIRETRIVQTAPGELVLQFTS
                     APGAATEDLRREQEHLRHGMHKLLGEEMRVSFEACRDLERDRSGKFKWIVSRLPPPGD
                     HPDGRRGSRPCPS"
     gene            complement(7463..8566)
                     /gene="wecB"
                     /locus_tag="PK634_02635"
     CDS             complement(7463..8566)
                     /gene="wecB"
                     /locus_tag="PK634_02635"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOW96665.1"
                     /translation="MNAAKKIALLYGARPNYMKVFPLWREMKAPGSVLRPLLIHTGQH
                     YDASMSDVFFRNLAMDPPDHALGVGAGSHAAQTGRIMQALEPVLGRERPAALVVVGDV
                     NSTMAGALVAVKMGIPVAHVEAGLRSGDRAMPEEINRVVTDAVADCLLASCRDAVDNL
                     RREGIPEERIHFVGNVMMDSLEAALPRLPESKIVETLGLAPRAYVLVTLHRPSNVDDP
                     GPLRAILGALEALSADRPVVFPVHPRTRKVMESNGVAPARAGFRCLDPLGYLDFLRLE
                     TEAGLVVTDSGGIQEETSYLGIPCLTLRENTERPVTITAGTNRLVPPRGGRLLEEARR
                     ALAQPPGPRPRIEGWDGRAASRIIPVLQGLVTA"
     gene            complement(8563..9750)
                     /locus_tag="PK634_02640"
     CDS             complement(8563..9750)
                     /locus_tag="PK634_02640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96666.1"
                     /translation="MTKQRPLHVVLVAAFPLQGAVPPEAIRPSCRDRPEHPAGWVRRL
                     AVALAGMEGVRVEVVAESRRLVRDYHARVDGLDVTYLRKAEPGRTDPWHLFLPGIRRL
                     RRVLAALRPDVVHGFGTEQAHGLIAVGAFAPAVLKIQGIVDQLQPYVPWPPWKTALWR
                     ALERRAVRRAAGIVANTAFSERWARKVSPGGRIAVIPNICPPAFFDVRPTHAEKLVLC
                     PGALSRIKGTETVLKAFAALGDPEARLVMSGWIGDADDYPGLARRLGVADRVEFPGHL
                     PAERIRELMGRARLVVVGSRMDTSPNVIMEAHAAGLPVAGTRTGGIPDMIADGVDGFL
                     SGVDDAAGLADAMRKLLDNAELCRRFGAAGREKVARLNEPAAVARAHLDLYEQVLGWG
                     EKP"
     gene            complement(9747..11207)
                     /locus_tag="PK634_02645"
     CDS             complement(9747..11207)
                     /locus_tag="PK634_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOW96667.1"
                     /translation="MILRRMFRDTFVYGLSGVVSRFIGFLLVPIYTRMLAPADFGLLD
                     LLTIASSIMVLLGGMQVESGIARGYYEARKAGTLATLVGTGIRLYTLGVAVVGVLGLA
                     AFQFWFVGRGGLTWREAAPLFVGLLPSQWLVLWLLLLRYEGRPYQYAVMALGDVLCTT
                     AFSVTGVCVLRLGVRGILWGMALSKILWATVGLVTRFSGSFRGWSRAEAGRILVYSVP
                     TVPNTLVNWIQNYGNRFILLGALSLAGVGVYGLANRLASPLMVAIWALRLAWDPLATE
                     LIGKPEAPGVYARALDAYLLGMTLACGLLGALGDLFVRVLAPASYAEAGRLVAGIAMA
                     NLWLGSLQVLMMGMNVVRQTYWGVIAFVAGLAANLLPLWLWIGAGGLPTAAWTLLAGS
                     LVTAEIALALSQRCFRIPYHYGVLRGSMAFSILASILFSAWPGLVPAGLGGAADTLLR
                     LAAAGAGWYLLVYLFVPREMRAELVRRVRHRLWKTA"
     gene            complement(11204..12757)
                     /locus_tag="PK634_02650"
     CDS             complement(11204..12757)
                     /locus_tag="PK634_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96668.1"
                     /translation="MTWGGVLWLTLGLAAYFAWQSFIVHRMAGTGSDHHFYVYYVDLL
                     RRGGRRLLYDFPNFLNRTPTVDPQYFHWVLSWAGGPGMLALLSRILNPLFSALSLAVF
                     AVFLASESAGSPVPWGANILLLAFTPQFQHSTNVRNYGLNTRGLGLLLFLLFSIACYQ
                     AGQAASWAWFAAAAGLAWLIIGCNIFAFQSLLLFAAVFLALFRSPVMAGAGLAGLLLF
                     TALHPRYAGHYFRQLLGFWSLYADTLAERFILLRRPSLWGDWVREIPSKWREKRSRAA
                     WYAYENSVAILVVFQPLALCAGAAWAMLPGGATALDLFSGRMAAASLILFLATSFRRT
                     RFLGSPERYVEMAMPFATALGADFLYRVGGFALVAVCAAGQVALSNLQPYMNVFRKKK
                     AAATDELQEFLRGVRARVEQDAGGRPVRFTSNNGEWGKYLLNADWRFVFFWPTVREIG
                     GIPIAELMEDYPFIKTDAVERLVRRYGVNYCLIDRHAGGTLGGGLEAAVLQESPRHVL
                     YRVAPREAA"
     gene            complement(12754..13524)
                     /locus_tag="PK634_02655"
     CDS             complement(12754..13524)
                     /locus_tag="PK634_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HOW96669.1"
                     /translation="MLRTRVIPCLLLRGESLVKTVKFDQFGYIGDPLNTCRIFNELEV
                     DEMTFLDITASREGRGPNYKILQEITNECFMPLAYGGGLRDTAAAERIFQIGFEKVVL
                     NTAPFERPALVTELSRAFGSQSVIVAVDVRRRLLGRYHVCGKSGTEEHRRDPVDWARE
                     VEGLGAGEILLTAIDREGTWNGFDVDLVRRVADAVSIPVIAHGGAGSVADIGAVVREG
                     HASAVALGSLVVYQKKGLGVLVNFPDRAELGRALEGAA"
     gene            complement(13529..14140)
                     /gene="hisH"
                     /locus_tag="PK634_02660"
     CDS             complement(13529..14140)
                     /gene="hisH"
                     /locus_tag="PK634_02660"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOW96670.1"
                     /translation="MITIVNYGLGNLGSIANMLKRIGVPSEIASEPGAVARAKKLILP
                     GVGHFDRAMERLQGTELRRVLDEKALGERVPVLGICLGMQLLTRSSEEGTLPGLGWVP
                     AVTVRFRPPEGSPLKIPHMGWNAVRVSTPSPLTEGFDGDERFYFVHSYHAVVDAPEHS
                     ILKARHGVEFDAAIRSGNVYGVQFHPEKSHRFGMKLLANFARI"
     gene            complement(14140..15312)
                     /locus_tag="PK634_02665"
     CDS             complement(14140..15312)
                     /locus_tag="PK634_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOW96671.1"
                     /translation="MTLTLKGTDLSARKVCARCIYDEGVRGIEFDAGGVCSFCRMVDG
                     LKAQYETGTPAGERRLMAIVEEIRRAGKGKPYDCVVGVSGGTDSSYLVAKALEWGLRP
                     LAAHYDNTWNTAIATENIRKVLGRLKVDLYTHVIDNKEADDIIRSFFLASVPDLDSAT
                     DIALAETLYRAASKYGVRYILEGHSFVAEGVSPLGALYMDGQYIASVHRRFGRLPMRT
                     YPNMPFAAFMKWTLFKRIRKVRPLWYIAYSKSDAREFLEREFDWQYYGGHHLENRITA
                     FHHSYYNPVKFGIDNRNNSLSASVRAGIMSRDEALRIYAAPPYMEPDLLPYFKKRLEL
                     TDAEFDRVMALPPKTFRDYPTYKRRFERLRPLFYLLAKSNLVPMSFYLKYCRKSTA"
     gene            complement(15330..17198)
                     /gene="asnB"
                     /locus_tag="PK634_02670"
     CDS             complement(15330..17198)
                     /gene="asnB"
                     /locus_tag="PK634_02670"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOW96672.1"
                     /translation="MCGIAGILHRNGAPASPAILQRMTDALAHRGPDGEGHWIEGGVG
                     LGHRRLAIIDLSPAGHQPMISADQRHVLIYNGEVYNFRELRAELESAGYWFRSRTDGE
                     VVLLALAAWGVKAFDRFNGMFALAFWDRKERTLLLARDRFGIKPLYYAEQGGVFAFGS
                     EQKAILANPAFERRLDKAALLEYFTFQNIFTDRTLLEDIRLLPAGHYATLDFKDPAAP
                     LRLTRFWDFHFQEPAEQASDEEYREELDRLFRQAVSRQLVTDVELGSYLSGGMDSGSI
                     TAIAAQTYPYMKTFTCGFDLHSASGMELSFDEREKAELMSYTFKTEHYEMVLKAGDME
                     RIFPKFAWHLEEPRVGQSYPNYYAAQLAGKFVKVVLSGGGGDELFGGYPWRYYRAVGG
                     SGFEDYVDKYYLYWQRLIDNSTLWALFAPIKDQVRDVWTRDIFRDVFRKHEHKLEAPE
                     DYINHSLCFEARTFLHGLLVVEDKLSMAHSLETRVPFLDNDLVDFAMRLPIRLKLANL
                     DNVLRADENDLARYYRRTSDGKVLLRQVMKRHIPTEIAEAEKQGFSAPDASWFKGESI
                     EYVKKLLLSPRARIYDLFDPAVARALIEEHLAGHVNRRLFIWSLLSLEHFLRAYLP"
     gene            complement(17212..18240)
                     /locus_tag="PK634_02675"
     CDS             complement(17212..18240)
                     /locus_tag="PK634_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW96673.1"
                     /translation="MNKALCALLRDPVARGPLTWDEAAGELASAGGGRYPVRNGIPRF
                     VLTEDAGQIQTRDAFGFKWAKRDTYDTPEFRAFAAEWYSRKYGFGDEAAMARFFAGRG
                     RILDVGCGSAFSSALYLRHPAWSGRAMWVGADISSAVDVAQERLRDVANTHFVQADAL
                     QLPFADGAFDTVFSEGVLHHTPSTRAALLSAARVVARGGEFLFYVYRKKAPLREYADD
                     YIRGQIAPLTDEQAWEAMRSLTRFGQALAELKASVTVPEDVPLLGIRAGAYDIQRLLY
                     YDVVKMYWNAKLSFEENVHVNFDWYRPRYAHRQTAEEVRAWCAEAGLEIARFHEEESG
                     FSVRAVRR"
     gene            complement(18237..19370)
                     /locus_tag="PK634_02680"
     CDS             complement(18237..19370)
                     /locus_tag="PK634_02680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOW96674.1"
                     /translation="MHKIPLIKPYITDEVKAKVCQVLDGGQLTEGATTREFEALCRQH
                     IGCAHALAACNCTVGMEMALRLFGVGPGDEVIVPDYTYSATAAVARFVGATMVLVDVD
                     PATMLIDYDALEAAVTPKTKAIIPVSMFGNPLDYNRLREIQGRGQFKIIEDSACSIGA
                     SWNGKKVGNWADISVFSFHPRKFITTGEGGLVTTNRDDWAAWMDSYKHFGMMRMAERE
                     DIQFGMIGTNFKMSNILAAVGAVQMKHIEELLAKRESLALRYREKLGGVEGITLPAVT
                     PGGRHSWQTCCVFVEDRNPIMKRMRADGIEVQVGNYALHMHPAFQPSAWCRHAGSLAD
                     SRRVFDRALALPLYYDLTESDQDIVIERLIREVRAERSKGGAT"
     gene            complement(19379..20368)
                     /locus_tag="PK634_02685"
     CDS             complement(19379..20368)
                     /locus_tag="PK634_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOW96675.1"
                     /translation="MDIKGKKIMVIGGAGLIGSHIVDQLLQTDVKEVVVYDNFVRGRM
                     ENLARALKDPRCSVFPLGGDVLQPDVLDTAIKGVDGVFHLAALWLLHCHEYPQSAFEV
                     NIRGTFNVLEACRRHHIQRLVYSSSASVYGDAVEEPMTESHPFNCWTFYGATKIAGEA
                     MMKAYHKRYGVKGVGLRYMNVYGPRQDYKGAYIAVMMKALDCIDRNEQPLVYGDGSQA
                     YDFIHVEDVARANLCAMTSECPVGFFNVGRGVKTSVKELIEVLLKLTGKQSLGINFKP
                     ATEYFVTNRVGCPKAAREKLGFTWKIDLEEGLKSLIDWRKAHIAEVEARRRLV"
     gene            complement(20463..21575)
                     /locus_tag="PK634_02690"
     CDS             complement(20463..21575)
                     /locus_tag="PK634_02690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOW96676.1"
                     /translation="MNVPLLDLKAQYAGIKAEIEPVLREVVESQYFILGPKVQECEKA
                     IAAYCSCARAVGVTSGTDALLVSLMAEGIGPGDEVITTPYTFFATAGSIARTGARPVF
                     VDIDPATYNIDPARIEAKITAKTRAIMPVHLYGQMADMDPIMEMANRRGLVVIEDAAQ
                     AIGSEYKGRRAGSIGHYGCFSFFPSKNLGCFGDGGIVTTNDAARADKLAILRAHGSKP
                     KYYHKIIGGNFRLDALQAAVVLVKLRHLDAWTLGRQANAKRYDKLFAAAGLTDKVALP
                     RVVANRHIFNQYVLRVPRRDELQNFLKDKGIGTEVYYPVPMHLQECFAYLGHKKGDFP
                     ESEKAALETVALPVYPELADAQAAYVVDSIKEFLSA"
     gene            complement(21631..22008)
                     /locus_tag="PK634_02695"
     CDS             complement(21631..22008)
                     /locus_tag="PK634_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOW96677.1"
                     /translation="MSLAKSFEELRIWQEARVLVVDVYSVFGKGTPAQRDLEFRTQAQ
                     AAVLSAMSNIAEGFERKTRVEFARFLDIAKASAGELRSLLYVAEDLGYLNSENAEALR
                     NKTATLSRGIAALTRSLRKRAAF"
     gene            complement(22093..22779)
                     /locus_tag="PK634_02700"
     CDS             complement(22093..22779)
                     /locus_tag="PK634_02700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOW96678.1"
                     /translation="MAPAAASGPAREYFVHETACVDQPCEIGAGTKIWHFSHVLPRVK
                     IGKKCILGQNVHIADGVVMGNNVKIQNNVSVYTGTVIEDDVFLGPSCVLTNVTNPRSQ
                     VNRHALYEKTRFKRGCSVGANATIVCGVTVGRYAFVAAGSVVTKDVPDYALVMGNPAR
                     QKSWMSRHGHRLGAPDKDGVMRCPESGFRYKEVEPGVLKCLDLDEEAPLPAELAKGEK
                     TYDEFKGGKV"
     gene            complement(23401..23748)
                     /locus_tag="PK634_02705"
                     /pseudo
     CDS             complement(23401..23748)
                     /locus_tag="PK634_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(23830..24333)
                     /locus_tag="PK634_02710"
     CDS             complement(23830..24333)
                     /locus_tag="PK634_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96679.1"
                     /translation="MSGSTPQQMRRRFIEAAGQMTQTLGVGRALGQVFTHIYFSRQPQ
                     SLDDLTQSLGISKGSASMTVRQLEQWGALRKVWIKGERKDYYEALDHFGRIIRRALLD
                     VIGRKMESGDHVLQDAEEEMQEAAAGSADDATDWTFLRDRVAKIRAFRGRAQRLWDSS
                     ILKMLLK"
     gene            complement(24651..25625)
                     /locus_tag="PK634_02715"
     CDS             complement(24651..25625)
                     /locus_tag="PK634_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96680.1"
                     /translation="MKLAPIISRFLLLAVLAVRPVAAESLATSAGPLGCAAPEYFFGW
                     ADNRTRVDHVFVLENRTNQALTVRIMDPDPAPTVARELVLAPDSRAQVNLGFSPRGLK
                     GEVTKDFAVELPDFPGQALTLRFRGEARQALQLEPERVAFGTIPVDAAVTQSVVLTFN
                     TSWPLKLHEAQSSSAAVRAAAGPNESGPGYRIAVSTVPPLPVGALSGTVRVLTDDPMQ
                     RDLEIPVSAAVELPLRVEPRELVVPADGVITGELRVVVQSPFRAFAVQEAIAPPSLET
                     SISGEGPAAYAITLRPVPGAVVSGDAYLRIRTDVAAQSDLLVPFRRAE"
     gene            complement(25689..29828)
                     /locus_tag="PK634_02720"
     CDS             complement(25689..29828)
                     /locus_tag="PK634_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW96681.1"
                     /translation="MSRSTIRIPWVMAAVMLVAASAWAAPYMSVNGANGDYLTVHYYI
                     DEPTDSETVYVDFYPNEANVTKAQIWHNVSRRDRATLGDQDATSDPGPDPAGTNYFVA
                     TTMTAGEGGKWYASLPANKTGAYRLNCRYKVSGDANWRWAGVRDTAVVVSPKKARDVI
                     LYELQANVINASGDSEGTRSTFEDFLNPTNYANLNHFTSIGVNTLWIQPIHPIGDHYC
                     VSSDPGSPYSIQNMWEVAPHHSRGNTRAASMVAFSNFAAAAKAQGLDFFFDTIFNHTS
                     WDAEIGRDPANPNNPAGNPSAKIKDTLPQWYSRYVSTDLPCGEYSYNQSLFQYWLPAT
                     SSSQIGPAPAERSDFGKWPDVADLYWGTYPALSNPQTDTDSYWDPNATGADVKRMVEY
                     FAYFGKYWIEKSGGALGGFRCDYAQGLPPQAWEYIINKTRQSKWDFIFMAESLDGGNV
                     SKRAGRHVDIINQNWVWQVLGGGGNTSGMRGVIDGTKTDYGYAGIMRGLINHDQSAPA
                     DKWYTFSRYAVGAVVDGAPQMYQGQELGYTDHWGFSQFRNQFDRWIPNILIWHNMLTL
                     WHNRDNVLQGAYSRVNKGRQRNIASRLHDQWYLNRTGGAGTHEQIFSVLKYEGSKYGW
                     DPAHQNVVLSFVNLTPWTGQSGTFNLAEVTAIYLNPTRYYNIKNLCADDPDAYLWAAP
                     GKTGQELINSGIYINMPADGNGNPETAFVQMLKLEEHGGGGGGDTSSVAWVGNTVHSP
                     TNGAIESSSSITVYTESYPTNAAVSGDVVYTSNGGTTWTNKALVKGAVVGQNDSWTCN
                     LGAFGACKTIQYAIVMRDANTNEEWDSNNDNDYFAYVNCPTVTLSNVNHTPGNGYITP
                     SDGIVVYVETAPLFLAVTGAVAYQVNGGNWTNKGLTYGGAAGQALNRNWWTCNLGAYP
                     ACSTVRYAVVAFDAATNEFWANNNTADYWAYVNCYDLAITNPPTDTQVANGVTTYTLK
                     GKAYGNLAGQLSWTNQATGATGVLATAASWSVADIPLATGANVIAVSGAYTSSAQETV
                     AWDNPTNASYNSGWTNNSNGGSGLGAWALTAGANAGQFRATLADGTNMSVDAAGWGMW
                     AHTDSASDAIRNFNSALAAGDSFSLRWDNNWVDDDPQGSVGFALQNSTNGNRFEFMFI
                     GGGTNYVINDSTSSRHTGIPWTGDGLNITFALTGADAYRLTVGATILTGTLAHAGTSI
                     SKFKSWNWRSGSGWQYNLYLGDMRVTRASTGTTPISDSVTITREGNPDDPDIDGDGIP
                     NDWETEHFLGPTNANAGAMAANGVNTVMETYIAGIDPNNPEAFWKVSPAGVPAVGLQV
                     TWPSLTGRVYQLDRATDLVAGFSELDSNIEADPPVNTYHDATATNPTPYFYRVQAEIA
                     P"
     gene            complement(30008..34171)
                     /locus_tag="PK634_02725"
     CDS             complement(30008..34171)
                     /locus_tag="PK634_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOW96682.1"
                     /translation="MKSMRAAKQTIVCWVLLLAVAAPALGNGNVYVSRFWHNHQPIYW
                     PEWNGNGSQTERIQYAWDSIVLKDGQKYDTSVGHPENNLSDIFGVDDRKAAYQGRPRD
                     SLANINSAGGFAISYSGSLIDNVRNLGANGQLGYGSGWWDGNRQARNWTTPSGARRLD
                     LVGFNYHHSLAPLLPKAVFRKELQVFKQAWWKAWGGNADLSDHSKGYFPTEMAFSHHM
                     IDVLADEGYQWSIVASHHLSRTCPTYLNSANPTGSWNIFSSPPNRADRLGPSPSSGWW
                     YSEPNPGNAAWNVSPFAYQLHKVKYVDPATGAEKSILVVPSDDVLSYRYGYASEGISK
                     IQTYISPFATDPSHPVMVMPATDGDNAWGGGNSSWMEATPQFFNDSGSAGYHQSTPQD
                     FFKAFSNSADTVHIEDGAWIFPEMDYGSPYFLKWIEPPVNATNLPACYPGTKIDLETP
                     GFALKFWSWAPVMSGANWCETAEQILADEGTNVQPWKIQCIYDWDGSYTSPNLVERAW
                     HVYLGGLDSGFNYYGGLGNDDEVKQSLATARAIGLLQTWVNARIANDRTAPTVLKPQR
                     FPYNPGWYTFGWFNNIPSDTRFLKKMGSEFYIWTHAYDVSGITSIVAKVRLDNDGANS
                     MANNQNETYAGGSDVGSWISVPMTKRVLPNTRSALNAAAANGQIDYFVTAPYIADYYF
                     AKITSASVPNFRGKLLDYYIEAVDTRGNTNRTDIQQVYVEDDGGGGVASYATFSDDPR
                     DCAPLAVTFSATGGVLQGVSPVYQQISFDSGTNWARYLMTSAGNNLWVCTNTAPDNAP
                     SALVWFENSAGTVVDSNNGLNWYTAIRDCDEPTGPGAAETAPPSPTGCDPVRIVYHPN
                     GGELKDVSPVHVHVGYNGWQGVILPNPAMTQSNANTWVYTYTPPAGTYQIDVVFNDGG
                     GDSGVWDNHDGADWHFNVSGCAGLAEGVVITNPAGDSLTVTTSVATIGGTAGAGIAGH
                     LKWTNALTGGTGSLAVASSWTIASIPVGDGTNLITVVGSNTSGQVVTKASDAADASAY
                     DPAWDSGDNAGTGFGAWTLYAEGANAGHFRATKTGNPTLTIGNYAWGLWANSQQMAEG
                     SRAFSNAVAVGDSLSFKFQNGGVDGTGTYGSSVGVAIQNAATNDLLRFLFAGGQANYT
                     LADSTGDRNTGLGWTSNGLSITLRMTNTTGYVLTVHPYGSATSTFTGTLISQADSAIR
                     RVRVWNYNSGVNGENNEGRNFYVNDLRVQEVQSLVVVTGDTVRIVRASDGNGDGLPDW
                     WQIEHWGSAGDPDAAPGADPDEDGFTNEQEYRLGTDPEEKSSGLVLRNGVVLNGQYPQ
                     INWISVGGRVYSLQVSTNLLDPEGGFVQFQEVTETAADGVETNRSATHNLPLPASGAR
                     HYRVRLLSP"
     gene            complement(34437..36167)
                     /gene="argS"
                     /locus_tag="PK634_02730"
     CDS             complement(34437..36167)
                     /gene="argS"
                     /locus_tag="PK634_02730"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOW96683.1"
                     /translation="MQNDVLTIEQVLSDWARGAFQAAFPDSIPDGLSVGVVPTADERF
                     GDYQCNAAMSLAKALKKPPREIASALAAAARPEAVAKAEVAGPGFVNLFLDPAWMAGR
                     LEQLGDDPRLGVPGPGRGRTVVIDYSSPNVAKPMHIGHIRSTVIGNALDRLHRFLGWR
                     VIADNHLGDWGTQFGLLIMGFRHFADPAAYETAPIEELERIYVKSYERSKEDPAWLDA
                     ARAELVKLQQGDPENRRLWQSFVEHSLREFNRIYERLGVSFDLVRGESHYNDRLPGVI
                     ERLEEKALARESEGALVVFLDDEKMPVCIVRKSDGAYNYATTDLATVAARAEEFAPDA
                     VIYVTDERQQLHFRQIFAIAKKMGVATRLVHVWFGLMRLPEGTFSTRQGNVIKLERLL
                     DEAQTRALAVVKAASPEMPAEQQGAVARAVGIGAVKYADLSQNPQSLVTFTWEKALAL
                     DGNSAPYLQYACARIASVLDKYRERFPGQDPAAHPIRLTEPIERRLAVRLLRFPEAVV
                     HAAEAFKPNLLADYLYDLAQTYSTFYQNVPFLKAEAGPRESRVRLCELTMKTLRQGLS
                     LLGIETPERI"
     gene            36334..36600
                     /locus_tag="PK634_02735"
     CDS             36334..36600
                     /locus_tag="PK634_02735"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOW96684.1"
                     /translation="MAPAKKKSEKEATPKAPKARKAAAPAAAAPRKRAAAKPALPAVA
                     AEERQKLVELEAYFVAEKDGFRGDSKNYWITAEAIVEARLAGKK"
     gene            36848..38101
                     /locus_tag="PK634_02740"
     CDS             36848..38101
                     /locus_tag="PK634_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96685.1"
                     /translation="MKNPFGRASILICLASVWLGLAPAHAGGLPVIPFIPIIPMTYSN
                     VEAPLQYVQYTPRETRTWTFRHPGAIAMRIRFAAIGFSTPEEGGYPEKRSTIEILNSQ
                     GAVVQTLTADANASFYSASVPGDVIQVRYTNKSNGGLGQSPAQAAPDTFSILGYSYLR
                     LLAAKEVGGAPLNYSVFTPTAGTAYNVSLHNQFQVPVTVKKPYTFNDYSTTKVDLFLN
                     GQFLGSSMGYRPAWDPWNIYFSAQLDMQQMIQLAAGNSTATTASASTLIIRETRYNGT
                     DTNATWKTQYQYKNFPLTFSDPRATHHGNITGQGDTGVGNFDRLVIHLPSRSMVSAWV
                     FSQSGGGSPFVDCDFKVMDANGVTLYEGNEAQVDGHGEGVSLMLGPGAYIFDLWRNSS
                     SCVGYAGGTGHMYIKCDPAAAVTDH"
     gene            complement(38102..39046)
                     /locus_tag="PK634_02745"
     CDS             complement(38102..39046)
                     /locus_tag="PK634_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW96686.1"
                     /translation="MTAGQDGRGKAGWTTAVRPGIKDGMGDPTPEPEAPAPDDWHLKL
                     FEKSLPLQARLAEIASMVGRVPVNQTWLDVSGDGVSSGQLRRLGGTWHTATPDDASAA
                     STLRLVGERVERIREGRLPFGDSLFDGVVILDQLERMPDDALLIAECHRVLKPAGRLL
                     VHAPHLKKRALLRPARRLLGMTDEALGRVRPGYTESVLFDLLKDGFDVQEARSYGRFF
                     VEALDLLAWRGERPLSLAAGADAGSLEKLVLRRARLYPVFQFAAGMDTLLGLTKGYRL
                     IVRARRRPWRPRRAPVLADGRSIAEAALGGKIGTAAPF"
     gene            complement(39043..44904)
                     /locus_tag="PK634_02750"
     CDS             complement(39043..44904)
                     /locus_tag="PK634_02750"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96687.1"
                     /translation="MPSNPGAAAPYTNWVTAATNIQQAIDVAADGDTVLVTNGTYFLS
                     AQINVTNGVAIRSANGFYVTTVNGNNSVRCFDVSNAAAVVEGFAITRGRTTARGGGVS
                     VAAGSVQNCVIVNNVGQNGGAGAYCRPGGVISNCVILSNIYSGSGNGAGGVWCSGGLV
                     RDCTIYSNSAGYASGGGAICSDGGTMENCIVAANTARNGGGIALWAYSVPATIRNCMI
                     YCNTASNTAGGISLESLGIMESSTVVGNWAPQGGGLVVYSTGTVFNSIIVFNEAVDWP
                     NYDAPEAAIQYTAAAPLPPGEGNTERLPDFVNPAAFDFRLRPGSAGVDAGTNQPWMVG
                     AADLNGRPRITGGLVDMGAYEFQPGEGTLVCNPMADTTEGFVPLQVIFTGWVSGTNTA
                     GLYYVWDFNNDGVPDRQGADTRIVTNTYNACGFYSVSLIVSNAAHETDTMVKADYIKV
                     GPAEAYVAPGAGHVFPFTNWVQAATNVQEAVDAGVGGTTVWLSNAVHSLTGQVVVRSG
                     LTIRGMDDPAAAIVEGNGAERCFLMDHRDATLRALTIRGGRAVNGGGVYSGQGGTITN
                     CIFTSNTATRAGGGFYGWNTRVTDSLFVSNTVAIAAYEEVFGGGACVWGPSRIENCAF
                     VDNEARSYDETFGGGVSLEAPAVISNCVLSGNLAESGQSYREAWGGGLYSYGAQVVDC
                     RVSNNTAQADQYDALGGGICAVEGAPIRDCDILANTAVSWSDGDDAEGGGIYGEEASI
                     TNCRILGNVASAPNGTAAGGGLYLDYGSVVTHSTVSSNKVEGGEYSQYGAGAALVWTS
                     RIAHCTVSGNWAETLAGNSVEGIGLWLDRSIAENCRINGNYTVAAGQVDGGGVYGAGG
                     STLRNLLVTDHVATNGGGIYLEGAALAQNCTVAGNEARDGGGLYLSGASSVNVIAYDN
                     EADRHPNLYALASTVDFSCAAPAPAGTGNTADAPDFMDPASGDYRLRPGSPCVDTGTN
                     LAWMAGAADLDGNPRVAGAGPDMGALEYQPGTGPLAANLLADPTQGFPGDLMIFQAIV
                     DGADTAGLYYQWDFNNDGVVDAQGLGLALVTNGFAACGYFSPLLTVSNAAGETAVVLK
                     PDYLKIGPATVFAATNGLHNFPYTNWVDAATNLQAAVSTGIGGTLVRVTNGLYELNEP
                     VVVTDGITIRAEAEAAAIVDGRGLARCFDLRHPQAALEGLLIRGGKAPQGAGVYMEGG
                     VLRRCVLAGNVATTEGGGAYLDMGGVVESSVICSNGGGGLYLFRGGTVSNCAIFANTN
                     AAGVGGGVRMFYGGVLRDSTVVSNRATHGGGVYGSGALVRNSAIRGNLASSFGGGLYV
                     DNNSVVDACTVAGNTASNNGGGAYGVGATFTNCIVCHNQAPAGTNTTGGTFVYSCAIP
                     LPSGTNNTTGDPEFADLANGNLQLLPGSSCLDNALLETWMSGAQDVAGNPRVSHGQPD
                     RGAYEFQFGPLTCNPVAGTRDGFLPLPVTLTGHVSGTNTAALNYVWDVDGDGTPDYQG
                     LGLSVVTHVYSNFGLYAVSLSVSNAAGETATLCKTNYVNVKPPVTYVAPGGAHASPFA
                     TWGTAATNIQAALNVALPGTVVLVTDGVYRVAARIMVTNPVTLRGVNGPAGTILDGGG
                     AAPCVKITHASAVVEGFTIRNGYSSAANNSEGGGVYMSAGTLQQCVVVSNRAGGGSAA
                     KGGGIYAFNATIRNCLLYGNQGMVAANGRGGGIYGWSFTVQNCTICSNTGRWGGGIYS
                     EGSVAVKNSIVMDNIVSVSDTNYYGTITFDHSCAAPQPAGVGNTNAAPLFMDSGNGDY
                     QLQVGSPCIDTGTNLPAVACDLEGVPRPLDGNNDGPAGWDMGCYERTNPMADSDGDGL
                     LDTNELAIGTSPIRADTDGDRANDGEELVAGTDPLDALSVFEISGLRVQGSGRVIRWS
                     SVAGKQYDLSRSTHPQGVFEGVFTNLPATPPLNVHTDQAAAGAGPWLYRVETE"
     gene            complement(45022..46326)
                     /locus_tag="PK634_02755"
     CDS             complement(45022..46326)
                     /locus_tag="PK634_02755"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96688.1"
                     /translation="MFAVGASAQTTRYVVPVNPGAAAPYTNWVTAATNVQDAVSAATA
                     GDTVWVSNGTYYLGSPLEITKGVTARSVNGFEATVLDAQYPVRTNRCLYMNHPAGVVD
                     GFTVRGGHAPGDYFDFPGNCGGGVFFNNGGCVRRCRITGNSATRDGGGVFFYDGSAGG
                     VLEHCEIISNSAQWVGGVCMGAVTGIVRNCVIAYNTASSRAGAARIGEGQMCHCTVVL
                     NNAGDRAGGIEGSGLIVNSILYFNTAVSDNNYSEGPVFETSCTTPDPGAGTGNITNDP
                     VFVDAAAGDFRLTTNSPCVDAGSPTNQLAGDINGIPRPLDGNNDGTNAWDMGAHEFVH
                     PEADSDADWLRDGDEILAGTDPLDPLSLLEVSRLRFQASAPAVSWSSVNGKTYALSRS
                     TNLPAGAWTPVQSGIAGAAPMNTVTDATAVGNGPWHYRIELE"
     gene            complement(46398..>48893)
                     /locus_tag="PK634_02760"
     CDS             complement(46398..>48893)
                     /locus_tag="PK634_02760"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96689.1"
                     /translation="TSRCFYLCHPAARVSALTIRGGRADYGGGVFFERYGLVSNCVVT
                     GNRSDNTGGGLYFENGGEATDCEIWSNSTRSGESWGGGACLEGGVLRRSRIHSHTNGG
                     VRIDGAGTLEACQIYSNRANSGGGVWALGGTVRNCLVIRNQAGSQGGGLYFSGSFSTL
                     ESSTVCKNSAGTSGGGLAHVSGNSAVQNSIVRFNSAPASPDYLAGAYQYTCAAPLPAG
                     AGNTDRDPEFADTAHDDYRLLPGSPCMDTGANLAWMTNATDLAGSPRILNGITDMGAF
                     EYAYGALECNPSADPLIGLEPLRVVLTGYAAGTNTSGLYYQWDYNGDSIWDDQGYGLG
                     VVTQLYEAAGLYTVALAVSNAAGETSARARTNLITVAPLIAYAAPGGTHQYPYTNWAV
                     AATDLAAAVGAAWHGSQILVTDGVYAIRSPLELAVAATLRSVNGPAATVVDAQNSGRC
                     LTLNHPEALAAGLTLTRGRAYQGGGVYFYGGTLSNCIASSCYATNRGGGVYVNSTGLV
                     TRCVLMCNTGDQGGGVYMNQGGGVVRDTRIQANYALTSGGGVNIGAQVLENCLIYSNS
                     CGGNGGGVYSRGGQIRNCTIACNRTYGTLWGGGYYAYTAANIVNTIVYGNIGPATYSN
                     YSGGAWTYSCAAPLPAGAGNIAGNPLFVDPDAYDFRLPTNSPCVDAGAPTNLPLSDID
                     GMPRPLDGNQDGVAGYDMGAYERVHPEADSDADWLLDTNELAIGTSPVEADTDGDWMD
                     DGAEVLAGTLPLDAASFLGLTQIVRQAGAAGVAVRWQSVDGKRYTLSRATNLAAGFDY
                     DVKTNILGIAPANTETDTTAVGSGPWNYRIELE"
BASE COUNT         7716 a        16973 c        16213 g         7991 t
ORIGIN      
        1 cgcgccgccg cacccaccag ccgctgcggg gaaagaaggc ctccgacggc cggctgaaat
       61 cggcccctgg aaaaacctgt tccgccagct ggagcaggat ctcgccgcgg ccccggcgcg
      121 agatgaaatc cagcgggatc acgcgccccc cgtcctcgtc gccgatcggg aagacatcgc
      181 cgccggcatc caggcacgcc gcgaccacgc gggccatccg ctccagacgc cggaggaagg
      241 ccgggcgcag cgattcccgc gcgaacagcg ccagcagcag cgccatctcc gtgaccagcg
      301 cgtggtaggg cagcgaggat tcgtacaatc ccccgtcccg gtaggtctgg cgcagcaggg
      361 ccttctgcat ctccgcgacg ccgaagcggc gccaccgacg cccatccgcc gtggccggaa
      421 aatgcagccc gatcacggcg agacccagga ggtcggccag ataatgattg gacgacacct
      481 tccgcgcctc gatccgctgc tccaccacct gtcccgccgt ccagagcccg tccagccagc
      541 ggtcgcggcc cacccgctcg ctgacgccgc cctcgaggag ggcgtccgcg caggcccagt
      601 tgaccgcgcg cagggccacc tccatggcgc agatccagtt gggcccgccg agatcgcggt
      661 tgtgctcttc ccagaggacg attttttccg cgatctcggc ggccgccgtc tcgcggcgcc
      721 ccgaaggcgt ccccgccgcc tgcagcacga aggcgtaccc gcggctgaac tcccacggca
      781 gcttgatgtc gccggtcaga tcgcgcgcgc cgttcggccc cgaggcggcg gccaggctgg
      841 cgaggtcctc cggattcaac ggcccgccgg agtggcagcg tccaagcagg aactcctcgg
      901 agaaatcccg gtggaacacg cgcgtccacg ggtacggctc cgcctgccat tcgcaggctt
      961 cgaaccaccg gcgcagcccg tccaccggca gggccgcaca ccggccctcg cccggcggcc
     1021 ggggccggac gacaagcgga ggctccgctc ccagcttcga ccggatcttc cgccacagga
     1081 aggccgggtg ccgggaccag gggctcaacc agagggcttt aagcaggcaa ggtcgcatgg
     1141 tccgcgatgg cctggtcgaa caggaccgcc agacgggcga gaaagacgtc cttgtcgaag
     1201 cggcgcacct cgtcctcgcg gaacggccac gggccgccct gctcgcggtg cgcccggaag
     1261 cgggccagcg tctcgcccac cgcctccgcg tcgtgcgggc agcacaggcc ggcgccggtc
     1321 cgctcaacga tctccgtgcc ctcgaagggc ggcgagatgt acaggatcgg cgtccgcgcg
     1381 cccatggcct cgtagatctt gccggggata tggattgtgt cctgcggcgc ctggacgatc
     1441 agcgcgacat cgctgcgcgt cagcgtttcg atcgcctgcc gatgcggcac gtggccgtag
     1501 tactcgacaa acgggcccag ccccatggcc tccaccgtct cccgcaggcg atccacaccg
     1561 atgatctcgc cgagccactg gaaccggaac tgccccggcc gtatcccggt ccgctccacg
     1621 aagcgccgga gcccctcgag aaagacgccg ggctgccggc tgaagtagaa cttgccgacg
     1681 tagctgacgg tcagcggccg accgggctcg cgcggcgggc ggggcgggta ctcggccgga
     1741 tcgaagaacc cgtaatccac gctgatctcg ggacgcagat cggcgtaccg tgcgcggaga
     1801 tagtcggcca ggcccgtgct gacgcacgtg atccgggtcg cgcgccggat cgccgtccgc
     1861 tccatccggg cgtgcagcca ccggtgcagc ggcgtgggcg ccttcaggtt gtcgtagaaa
     1921 ttggacgtcc agaggtcccg gtactccacg acgcagggaa gcccgaactc cgccgcgacc
     1981 cgcgtggcca cccgcatgtc cgtgcgcggc gcgagcgatg cgtacacggc gtccacgcgt
     2041 tcgcgccgca ggacctcgcg cgccgcccgg agcgcggccg ggccccagag catctgcggg
     2101 tcgggcacaa gcagccactg cttgatccac gtgctgaaac ccgtccgcat cgacaccagg
     2161 tccttcggcc cggccgccgg ggccggcgcg aacgagccct cgcggccgcg cagccggcgg
     2221 tacgccttcg ccaggtcaag cccccggacg ggcacgagtt cgtagtcgtc cgacgccggc
     2281 cactggtcgc ggttccggtc gtgggtgacg atgacgggct tatagccgtg ccggtgcaga
     2341 ttgcgcagcg cgcggcggac gcggatgtcc cccatggaac tcccgcccat gaagaagtac
     2401 gcgctgacga ggaccttctt catgcctgcg cggcgtccag cacgtcccgc acccgcgcct
     2461 cgcggccgga ttcaaggctc tgctgcgcgg ccagggtcag ggcggacgaa tagagcaggt
     2521 cttcgaagga gatcggcggc ggcccgcccc ggcgcgcgga atccaggaac gcctgcacca
     2581 gggccgcctg ccccttgtcc tgctgcggga gcttttcgcg gagcgcccgg gccggcccgt
     2641 accactccgc ccggcggaaa tcgtcgagcc gcgcgacgcc cgggccggcc aggatctcga
     2701 ggtgctcctt cggcagcagc ggcgagccct gcgcgctgta tgcgagggag gcgacgctgc
     2761 cgttgtcgaa gctcacgagg gcggcgaaat tcgcggtggg cagcaggtcg gcgcgatcgc
     2821 ccttcagcgc gcgcgtgacc acggagaccg gccggccgcc ggccaggcag caggccaggt
     2881 cgaagaagtg gcagccctcg cccacgagcc gcccgccgcc gatcttcgga tcctgcgtcc
     2941 agtggcgggc cggcgccggg ccggcgttca cccggcactg gatcatgacc ggcccggcct
     3001 cgccgaggcg cgcccggatg aactgcgtga gcgggctgaa gcgccggttg aacccgacca
     3061 ccaggcgggc ttccgggccc gcgcgcacgg cggccgccac cgcgcgcaat tcctcgacgg
     3121 tcagcgcgag gggtttctcc acccagacct gcttgcccgc cttcaggcct tcgaggacga
     3181 gcgaggcatg ggaatcgtgc cgcgtcgtga tgaacacgac ctgcgtgtcg gcatcgccga
     3241 ggacctccgc ggcgccggcc gccgcgaagg cgaaaccgaa gtcccgggcc gcggcggcgc
     3301 tcgcgtcgac gcgctggtcg gccacgccgc gcaaccgcgc gtttccgttc cggctgacga
     3361 ccgggaggag catggccctc gcgtaggagc cggccccgat gaagccgacg cccagcgcgc
     3421 ccggcgccgc gcggggcgcc gcgggccggt ccaccctcgg cgcgcgggga gcgggcgcct
     3481 cgggatactg gagcacgatc cccacccgct cttccgccct gttcttcagc gcgtcgtagg
     3541 cgcgcagggc gtcggcgaag gcgaaccggt gcgtggtcag gttttccggc cggatttttc
     3601 cctcctgcgc gagctggagg aaggcttcca tgttgcgctg ctcggtgaag cggacgtact
     3661 ccgccggata gtcgagcccg ccctcctcgt aggtcgggtc gtagcggccg ggaccgtagg
     3721 agcgcgacag gcaaaaggac agctcctttt caaaaaagag ctggcgcggg atgtccatcc
     3781 ccgtggagcc gaccatgacg acgcgcccgc gcgaacgggc cagccggccg gccagttgga
     3841 ccggctcgct gccggacgcg gcggcggcga tgatgaccgc gtccgcgccc agcccgcgcg
     3901 tcatgcgcaa cacggcctgg cccgccccgg ccgggtcggc cgtctcgtcg cagcccgccg
     3961 tccggcccca cttccgcgcg ttctcggccg ggtccacgcc ggcgacccgg cagcccgcgg
     4021 ccttgagcag ctggacggtg agcaggccga ccaagcccag cccgatcacg gtcacgccct
     4081 cgccgagcgc cacggcggcc tgccgcacgc cctgcatcgc gatggcgccg agcgtcgtgt
     4141 aggccgcggc ttccagcgac acgccctccg gcacgggcgc gcagagattc tgcgggacgc
     4201 aggcgtactc cgcatgcacc gcgtagccca cgcccgcgca ggccaccggg gcgcccggcg
     4261 ggaagcggtc cacgccggcg cccgcccgct ccacgacgcc cgcgcagctg taacccagcg
     4321 gcgcgtaacc ggtcttcttc gcgccgacct ttttcatcag cgcggacagc ccctgatcgc
     4381 ggagcatccg aaaggcgcgg ccgatgagct ccggccgttc cgagaccttc tgcagcgccc
     4441 cggcctgggc cgccgccagt tgcgcgctct ccgtgccggc gctgatgatg gatgccgcgt
     4501 tgcgcacgag cacgaagccg gacggcgcct ccggcacggg cacctcgacc accgtcaccg
     4561 ccgcttgcgt caggtcctgc agcacctgtt tcatggggcc tccgcgttgg acgctatctc
     4621 tatgtggcct ttccattccg agtgaggctg gaaattcagg cgcggatcga tgtcccgcgc
     4681 gctgaagtac tccgcgcccg gcgcaacccg ctctccttgc gcgacgcgaa tttcctccgg
     4741 cgcggaaaaa ccgaagttcc ggcggcggtc tttcagcacg tcgaactgcc gtatccgggc
     4801 cgggtcgaag cccatgagcc ggctggcgac caggtccacg gccagcggat tctccccgcc
     4861 cacgaggcac ccgctgggcc gggcggtcgg cgccagcggc ccgtcgcctt cccctccgac
     4921 gatcccgtcc accaggcaga acatccggcg ctggacgcga tcccgcagca cgccgtcgcg
     4981 gtccgcgaag aagaggatcc gggccatgtc cgaggtcatg cgccaggccg aatcgttccg
     5041 gtgccagttc ccggcgtcca cgatcggctc gccggccgtc gcgcccacga agggccgaaa
     5101 gaggcggcgc cagaccccgc gcgcggcggc gtagaccgcg tcgccccacg cggtctgccg
     5161 cgccagcgcc cggtcgaaga gccagcgctg gacgcgcacc ttcagccgga cgccggccgc
     5221 cagggcgtcg ggcgcctggt cgcccccggt gcgcggcgtg cccagccggt aatggatcag
     5281 gtagttcttg ttcgtgttga ttcccacgag ccccttgagg ttcagggtga cgcccacctt
     5341 cttgtgcgtc ttgagcttcg gcacggagat cagcgcgtcg gcccggagga cggtgttgga
     5401 aatgcagtac tcgtgggtct cgccgtggtg gtggcggatg gtttcccgcc ggtcgtaatc
     5461 ggcgccgtag taccgcgcgc tgtccatccc gtagaacgcg ctttcccggc cgaggttgat
     5521 gacggtgctg cccgccggat cgccggggag cggcttgcgg ttgtgcgcgt aggcaggctt
     5581 ccggttgtcc accagggcga aattccgaag atcgcagatc tcgagcggga agcccgccgc
     5641 ccggcggtag aaatccgcga cggcgtccag gcgcagggcc cgcatgagtt cgtcccagtc
     5701 gcagcccatc tccggcgcgt ccgcgatcac caggcggccc cggccgcgca gggcgatgaa
     5761 gcagtagtcc gcgagggccc gcaggacgga gggatgcgtg acgacggcga agagatcgcc
     5821 ccccccgagg ttccggctga tcacgaaatt cggcttgagg accaccgtgt cgcccggccg
     5881 gacgatcgcc cccagcggat tccaatccgg cgagcccgcg tgggcctcgt ccatccccag
     5941 ccgcatcagc agccggcgca ccgccgcgta caccgggttc ggccccgccg tgtccgggaa
     6001 gggcgcttcc ggataccgct cgccgggatg gaagggcggc tcgtccgggt aggagggcgt
     6061 cgtctgttcg acggctacga ggggcatggg cggctcccgc ggcggccgtc cgggtggtcg
     6121 cccggcggcg gcaggcgcga cacgatccat ttgaatttcc cggagcggtc gcgctccagg
     6181 tcccggcagg cctcgaagga gacccgcatc tcctcgccca gcagcttgtg catgccgtgg
     6241 cggagatgct cctgctcccg gcggaggtcc tccgtcgccg cgccgggcgc ggaggtgaac
     6301 tgcaggacaa gctcgccggg ggcggtctgc acgatgcgcg tttcgcggat cgccttcagg
     6361 cccttgaacg ggtacgtgat gatggccggc ccgatccaac cccgcgccgg gctgtacacg
     6421 taatcctcct gccgcccgac cagttcctcg accagcggcc ggtacgggcg gcggcaatcc
     6481 gggcaggcgt cggcggacac gccgcgcacg gtatcggtca tgcggtagcg gatgaagggc
     6541 gacacgtcgt tgtgcaggtt ggtggccatc acctcgccgg tgtccggcga gacctcgagg
     6601 aagccgtagg gcgacaggac gtggtacgcg ggccggcgct cgcaccagcc ggccaggatg
     6661 gcgcgctccg cgcaaccgta gtgcgcaaag agccgggcgt cggggaacgc ctcgcggatc
     6721 gcggccagct gccagtcata caccacctcg gaggcgagca ggatggcgcg gggttgcggg
     6781 aaatccagcg cctcgtggcg gatcgtcgag gccagcaggc tcaaggcgct cgggtagccg
     6841 tggaagaact cggcgcggca ccggcggatc tccgcggcgt agcgctccag gcgcgcgcgg
     6901 tcgaacccca ggatggcgaa ccgcaccgtg ttggagtgcg ggcggcgatc cacgccggcg
     6961 gggtccagcg tcagcccccg gaaggtggcc cggacgctgt gcgccggatc gtagccgacg
     7021 cgcttccact ggtggcagat gctcgcccac tcgcgccaac ggtcgccggg cggatgcagg
     7081 aattgcagcg ccatgcccgt cgtcccggaa gtgcagccgc gctcggcctt cgccgcccgg
     7141 tcgcggcagg gccagcgatc catgttctcg cgcagctcgc ggcgggtcgt cgccggcacc
     7201 gcggcggaga aatcggcgag cgagcggatg tcgtccggcc ggacatcctt gagcttttcc
     7261 gcgtagaacg ggctgccggc cctggcgctc gacagcaggc ggcggatgcg ctcgagctgg
     7321 tacgcctcca tccgctcgac gggccaggtc tccgcgtcct cgtagaacgc gtaccacttc
     7381 cagaaggcgg cgggccaacg ccgccgcgcc ggcaccaggc tgtacaggcg cgacaggaac
     7441 tcgcgaacgg ccgggcttcc cttcatgcgg tcaccaggcc ttgcagcacg gggatgatgc
     7501 gcgaggccgc gcgcccgtcc cagccctcga tgcgcgggcg ggggccgggc ggctgcgcga
     7561 gggcgcggcg cgcctcctcc agcagccgtc cgccgcgggg aggaaccagc cggttcgtgc
     7621 cggcggtgat cgtgaccggt cgctccgtgt tctcgcgcag cgtcagacac ggaatgccaa
     7681 ggtagctggt ctcctcctgg atgccgcccg agtcggtgac gaccaggccg gcttccgtct
     7741 cgaggcgcag gaaatccagg tatcccagcg gatccaggca gcggaatccc gcgcgggcgg
     7801 gggcgacgcc gtttgactcc atgaccttgc gggtccgcgg atgcaccggg aacacgaccg
     7861 ggcggtcggc ggagagcgcc tccagcgccc cgagaatcgc ccggagaggg ccgggatcgt
     7921 ccacgttgct cgggcgatgc agcgtgacca ggacgtaggc tcgcggggcc agccccagcg
     7981 tctccacgat cttcgactcc ggaagccgcg gcagcgcggc ctccaggctg tccatcatca
     8041 cgttgccgac aaaatggatc cgctcctccg ggatgccttc gcgccggagg ttgtcgaccg
     8101 cgtcgcggca gctggccagg aggcagtccg ccacggcgtc ggtcacgacc cggttgattt
     8161 cctcgggcat cgcgcggtcg ccgctgcgca ggccggcctc gacgtgggcc accgggatgc
     8221 ccatcttgac cgccaccagc gcgcccgcca tcgtggaatt gacgtcgccg accaccacga
     8281 gcgccgcggg gcgctcccgc ccgaggaccg gttccaacgc ctgcatgatc cggccggtct
     8341 gggccgcgtg cgagccggcg ccgacgccga gcgcatggtc cggcgggtcc atcgcgaggt
     8401 tccggaagaa cacgtcggac atgctggcgt cgtagtgctg gcccgtatgg atcagcagcg
     8461 ggcgcaggac cgagcccggc gctttcatct cccgccacag cgggaagacc ttcatgtagt
     8521 tcggccgggc cccgtagagc agcgcgattt tcttcgcggc attcatggct tttctcccca
     8581 cccgaggacc tgctcgtaca ggtccaggtg cgcccgggcc acggccgcgg gctcgttgag
     8641 ccgggccact ttctcgcggc cggcggcgcc gaaccgccgg cacagttcgg cgttgtccag
     8701 cagtttccgc atggcgtccg ccaggccggc ggcgtcgtcc acgcccgaca gaaacccgtc
     8761 caccccgtcg gcaatcatgt ccgggatgcc gccggtgcgg gtgcccgcca ccggcagccc
     8821 ggcggcgtgc gcttccatga tcacgttggg cgaggtgtcc atgcgggacc ccacgacgac
     8881 gagccgcgcc cgccccatca gttcgcggat tcgctccgcc ggaagatggc cgggaaattc
     8941 cacgcggtcg gccacgccca gccggcgcgc gaggcccggg tagtcgtccg cgtcgccgat
     9001 ccagcccgac atcacgaggc gggcctccgg atcgcccagc gcggcgaagg ccttcagcac
     9061 cgtctccgtg cccttgatcc ggctcaacgc gccggggcag aggaccagtt tttccgcatg
     9121 cgtggggcgg acgtcgaaaa aggccggcgg gcagatgttc gggatcacgg cgatgcgtcc
     9181 gccggggctc accttccgcg cccaccgctc cgagaaagcg gtgttggcga cgatccccgc
     9241 agcgcggcgg acggcgcgcc gctccagcgc ccgccacagg gccgtcttcc acggcggcca
     9301 gggcacgtac ggctgcagct ggtccacgat gccctggatc ttcagcacgg cgggggcgaa
     9361 ggctccgacg gcgatcaggc cgtgcgcctg ctccgtgccg aacccgtgca cgacgtcggg
     9421 gcgcagggcc gcgagcaccc ggcgcagccg gcggatgccg ggcaggaaca ggtgccaggg
     9481 atcggtgcgg ccgggctcgg ccttgcggag gtaggtcacg tccagaccgt ccacgcgggc
     9541 gtggtaatcc cggaccagcc ggcggctctc cgcgaccacc tcgacgcgca cgccctccat
     9601 cccggccagc gccacggcca ggcggcgcac ccagcccgcc gggtgttccg gccggtcgcg
     9661 gcaggacggc cgtatcgcct cgggcggcac ggcgccctgg agcggaaagg ccgccaccag
     9721 gaccacgtgt aggggacgtt gctttgtcat gctgtcttcc acaagcggtg gcggacccgc
     9781 cgcacgagct cggcgcgcat ctcgcgcggg acgaacaggt ataccagcag gtaccagccc
     9841 gccccggcgg ccgccaggcg caggagcgtg tccgccgccc cgcccagccc ggccggcaca
     9901 aggcccggcc aggccgagaa gaggatcgac gccaggatcg agaaggccat cgagccgcgc
     9961 agcacgccgt agtggtaagg gatccggaag cagcgttggg acagggccag cgcgatttcc
    10021 gccgtcacga gcgaaccggc cagcagcgtc caggccgcgg tcggcaggcc gcccgcgccg
    10081 atccagagcc acagcggcag caggttggcg gcgagcccgg cgacgaacgc gatcacgccc
    10141 cagtaggtct ggcgcacgac gttcatcccc atcatcagca cctgcagcga accgagccag
    10201 aggttggcca tggcgatacc ggccaccagc cgcccggcct cggcatagct cgcgggcgcg
    10261 agcacgcgca cgaacaggtc gcccagcgcg ccgagcaacc cgcaggccag cgtcatgccc
    10321 agcaggtagg cgtccagggc ccgggcgtac acgccgggcg cctccggctt gccgatcagc
    10381 tccgtcgcca gcgggtccca cgcaagccgc agggcccaga tggcgaccat cagcggcgag
    10441 gccagccggt tggccagccc gtagacgccg acgccggcca gggagagcgc gccgaggagg
    10501 atgaaccggt tcccgtagtt ctggatccag ttgaccagcg tgttggggac cgtcggcacc
    10561 gagtagacca ggatgcggcc ggcctcggct cggctccagc cgcggaagct ccccgagaag
    10621 cgcgtcacca ggcccaccgt cgcccagagg atcttcgaga gcgccatgcc ccagaggatg
    10681 ccccggacgc cgaggcgcag gacgcagacg ccggtcacgc tgaacgcggt cgtgcacagc
    10741 acgtcgccga gcgccatgac cgcgtactgg tagggccgcc cctcgtaccg caggagcagc
    10801 agccacagca ccagccactg gctcggcagc agcccgacga agaggggcgc cgcctcgcgc
    10861 caggtcagcc cgccccggcc gacgaaccag aattggaacg ccgccaggcc cagcacgccc
    10921 acgacggcca cgcccagcgt gtagaggcgg atccccgtgc ccacgagggt cgccagcgtc
    10981 cccgccttcc gggcctcgta gtagccgcgc gcgatccccg actccacctg catcccgccg
    11041 agcagcacca tgatggagga cgcgatcgtc agcaggtcca gcagaccgaa gtccgccggc
    11101 gccagcatcc gcgtgtagat gggcaccagc aggaaaccga tgaaccggct gaccacgccg
    11161 gacaggccgt agacgaacgt gtcgcggaac atccggcgca ggatcacgcg gcctcccgag
    11221 gcgccacgcg gtagaggacg tggcgcggcg actcctgcag caccgcggcc tccagcccgc
    11281 cccccagcgt cccgccggcg tgccggtcga tgaggcagta gttcacgccg tagcgccgca
    11341 cgaggcgttc gaccgcgtcc gtcttgatga acgggtaatc ctccatcaac tcggcgatcg
    11401 gaatgccgcc gatctcccgg accgtcggcc agaagaagac gaaccgccag tccgcgttga
    11461 gcaggtactt gccccactcg ccgttgttgc tcgtgaagcg cacgggccgc ccgccggcgt
    11521 cctgctccac gcgcgcgcgg acgccgcgga gaaactcctg gagctcgtcc gtcgccgccg
    11581 ccttcttctt ccggaagaca ttcatgtacg gctgcaggtt ggacagggcc acctgtcccg
    11641 cggcgcagac ggcgaccagc gcgaagccgc cgacgcggta caggaagtcc gcgcccagcg
    11701 ccgtggcgaa cggcatggcc atctccacgt atcgctcggg cgagcccagg aaccgggtac
    11761 gccggaagga cgtcgccagg aacaggatca gcgaggcggc cgccatccgg cccgagaaca
    11821 ggtccagggc ggtcgctccg cccggcagca tcgcccaggc tgcgcccgcg caaagggcca
    11881 ggggctggaa gacaacgagg atcgccaccg aattttcata ggcgtaccag gcggcccgcg
    11941 agcgtttctc cctccacttc gaagggatct cccgcaccca gtcgccccac aggctgggcc
    12001 ggcgcaggag gatgaagcgc tccgccagcg tgtccgcgta cagggaccag aacccgagaa
    12061 gctgacggaa ataatggccc gcgtaccggg ggtggagcgc ggtgaacagc aacaggcccg
    12121 cgaggcccgc gcccgccatg accggcgacc ggaacagcgc caggaaaacg gcggcgaaga
    12181 gcaggagcga ttgaaaggcg aagatgttgc agccgatgat cagccaggca aggcccgcgg
    12241 ccgccgcgaa ccaagcccag gacgcggcct gcccggcctg gtagcaggcg atggaaaaga
    12301 gcaggaacag cagcaggccg agcccgcgcg tgttgagccc gtagttacgg acgttggtcg
    12361 aatgctggaa ctgcggcgtg aaggccagca gcaggatgtt ggcgccccac gggaccgggg
    12421 aaccggccga ctcggacgcc aggaagaccg cgaacacggc cagcgacagc gcgctgaaca
    12481 gggggttcag gatccgcgag agcagcgcga gcatcccggg cccgccggcc cacgaaagca
    12541 cccagtgaaa atactggggg tcgaccgtcg gcgtgcggtt caggaagttc gggaagtcgt
    12601 acaggagccg gcgaccgccc cggcgcagca ggtccacgta atagacgtag aaatggtggt
    12661 cgctcccggt ccccgccatg cgatgcacga tgaagctctg ccaggcgaaa taggccgcca
    12721 ggccgagcgt cagccacagg acgccgcccc aggtcaagcg gccccctcca gcgcccggcc
    12781 cagttcggcg cggtccggaa aattcaccag cacgcccagc ccctttttct ggtagaccac
    12841 caggctgccc agcgccaccg ccgaggcgtg gccctcgcgc accacggccc cgatgtccgc
    12901 cacgctcccc gccccgccgt gcgcgatcac cgggatgctt acggcgtccg cgacccggcg
    12961 gaccaggtcg acgtcgaagc cgttccacgt gccctcgcgg tcgatcgccg tcagcaggat
    13021 ttccccggcg ccgagcccct cgacctcgcg ggcccagtcc accgggtcgc gccggtgctc
    13081 ctcggtcccg gacttcccgc agacgtggta ccggcccagc aagcggcggc gcacgtcgac
    13141 cgcgacgatc acgctctggc tcccgaaggc gcgggagagc tccgtgacga gggcgggccg
    13201 ctcgaacggg gcggtgttca gcacgacctt ctcgaacccg atctggaaga tccgctccgc
    13261 ggccgccgtg tcccggaggc cgcctccgta cgccagcggc atgaagcatt cgttggtgat
    13321 ctcctgcagg atcttgtagt tcggcccccg cccctcgcgg ctggcggtga tgtcgaggaa
    13381 cgtcatctcg tccacctcga gctcgttgaa gatgcggcag gtgttcagcg ggtcgccgat
    13441 gtagccgaac tggtcgaact tcacggtctt gacgagcgac tcgccgcgca gcagcaggca
    13501 ggggatgacg cgggtgcgaa gcatggggtc aaatccgggc gaagttcgcc agcagcttca
    13561 tgccgaagcg gtggctcttc tcggggtgga actgcacgcc gtacacgttg ccgcttcgga
    13621 tcgcggcgtc gaactccacg ccatgccggg ccttcaggat cgagtgctcc ggcgcgtcca
    13681 cgacggcgtg gtaggagtgg acgaagtaga accgctcgtc gccgtcgaag ccctccgtca
    13741 gcgggctcgg cgtggagacc cgcacggcgt tccagcccat gtgcgggatt ttcagcggcg
    13801 atccctccgg cgggcggaaa cggacggtca cggcaggcac ccacccaagg cccggcaggg
    13861 tcccctcctc gctcgaccgc gtcaggagct gcatcccgag gcagatgccc agcacgggca
    13921 cgcgctcgcc cagcgccttc tcgtccagca cgcgccgcag ttccgtgccc tgcaggcgct
    13981 ccatcgcgcg gtcgaaatgc ccgacgccgg gcaggatcag cttcttcgcg cgggcgaccg
    14041 cgcccggctc ggacgcgatc tccgacggca cgccgatgcg cttcagcatg ttcgcgatcg
    14101 agccgaggtt gccgagcccg tagttgacga tcgtgatcat caggccgtgc ttttccggca
    14161 gtacttcagg tagaagctca tcggcacgag gttcgacttc gccagcaggt agaacagcgg
    14221 gcgcagccgc tcgaagcgcc tcttgtaggt cgggtagtcg cggaacgtct tcggcggcag
    14281 ggccatgacc cggtcgaatt ccgcgtccgt cagttccagc cgcttcttga agtagggcag
    14341 caggtccggc tccatgtacg gcggcgccgc gtagatgcgc agggcctcgt cccggctcat
    14401 gatccccgcg cgcaccgagg cggacaggct gttgttccgg ttgtcgatcc cgaacttgac
    14461 ggggttgtag tagctgtggt ggaaggccgt gatgcggttc tccaggtggt gcccgccgta
    14521 gtactgccag tcgaactcgc gctcgaggaa ttcgcgcgcg tccgacttgg aataggcgat
    14581 gtaccacagc ggccggacct tccggatgcg cttgaagagc gtccatttca tgaaggcggc
    14641 gaagggcatg ttcggatagg tccgcatcgg cagccggccg aaccggcggt gcaccgaggc
    14701 gatgtactgc ccgtccatgt acagcgcgcc cagcggcgaa acgccctcgg cgacgaagga
    14761 atgcccctcg aggatatacc ggaccccgta cttcgacgcc gcgcggtaca gcgtctccgc
    14821 cagcgcgatg tcggtcgcgg aatcgaggtc cggcacgctc gcgaggaaga aggagcggat
    14881 gatgtcgtcc gcctccttgt tgtcgatgac gtgcgtgtac aggtccacct tgagccggcc
    14941 gagcaccttg cggatgttct cggtcgcgat cgcggtgttc caggtgttgt cgtagtgcgc
    15001 cgccagcggg cgcaggcccc actcgagggc cttggcgacc aggtaggagg agtccgtgcc
    15061 gccgctcacg ccgacgacgc agtcgtaggg cttccccttc cccgcccgcc ggatctcctc
    15121 gacgatggcc atcagccggc gctcgcccgc cggcgtgccg gtctcgtact gcgccttcag
    15181 gccgtccacc atgcggcaga acgagcagac gccgccggcg tcgaattcga tcccccgcac
    15241 gccctcgtcg tagatgcagc gcgcgcagac cttgcgggcg gacaggtccg tgcctttcag
    15301 ggtgagggtc atcgggggcg gccgcgggat cagggcaggt aggcccgcag gaagtgctcg
    15361 aggctcaaca gcgaccagat gaacagccgc cggttcacgt ggccggccag gtgctcctcg
    15421 atcagcgcgc gcgccaccgc cgggtcgaac aggtcgtaaa tccttgcgcg ggggctgagg
    15481 agcagttttt tcacgtactc gatgctctcg cccttgaacc agctcgcgtc gggcgccgag
    15541 aagccctgct tctccgcctc cgcgatctcc gtcgggatgt gacgcttcat cacctgccgc
    15601 agcagcacct tcccgtcgct ggtccggcgg tagtagcgcg ccaggtcgtt ctcgtcggcc
    15661 cgcaggacgt tgtccaggtt ggccagcttg aggcggatcg gcagccgcat ggcgaagtcc
    15721 accaggtcgt tgtcgaggaa cggcacgcgg gtctccaggc tgtgcgccat gctcaacttg
    15781 tcctccacca ccaggagccc gtgcaggaac gtgcgcgcct cgaagcagag cgagtggttg
    15841 atgtagtcct cgggcgcctc gagcttgtgc tcgtgcttgc ggaacacgtc gcggaagatg
    15901 tccctcgtcc agacgtcccg gacctggtcc ttgatcgggg cgaacagggc ccacagcgtg
    15961 gagttgtcga tcagccgctg ccagtagagg tagtacttgt cgacgtaatc ctcgaacccg
    16021 gacccgccga cggcgcggta gtaccgccac gggtagccgc cgaacagctc gtcgccgccg
    16081 ccgcccgaca ggaccacctt gacgaacttg cccgcgagct gcgcggcgta gtagttcggg
    16141 tagctctggc cgacgcgcgg ctcctcgagg tgccaggcga acttcgggaa gatgcgctcc
    16201 atgtcgcccg ccttcaggac catctcgtag tgctcggtct tgaaggtgta cgacatcagc
    16261 tcggccttct cccgctcgtc gaacgacagc tccatgcccg aggccgagtg caggtcgaac
    16321 ccgcacgtga aggtcttcat gtacgggtag gtctgcgcgg cgatcgcggt gatcgagccc
    16381 gagtccatgc cgccgctcag gtagctgccc agttcgacgt ccgtcacgag ctgccggctg
    16441 accgcctgcc ggaacagccg gtccagctcc tcgcggtact cctcgtcgga cgcctgctcc
    16501 gccggctcct ggaaatggaa gtcccagaag cgggtcagcc ggagcggcgc cgcgggatcc
    16561 ttgaagtcca gcgtcgcata gtgccccgcg ggcaggagcc ggatgtcctc cagcagcgtg
    16621 cggtcggtga agatgttctg gaacgtgaag tattccagca gcgcggcctt gtccagccgc
    16681 cgctcgaagg ccgggttcgc caggatcgcc ttctgctcgg agccgaacgc gaagacgccg
    16741 ccctgctcgg cgtagtagag gggcttgatg ccgaaccggt cgcgcgcgag cagcagcgtc
    16801 cgttccttcc ggtcccagaa cgccagggcg aacatcccgt tgaaccggtc gaaggccttg
    16861 acgccccacg ccgccagggc cagcagcacc acctcgccgt ccgtccgcga gcggaaccag
    16921 tagccggcgg actccagctc cgcgcgcagc tcgcggaagt tgtacacctc gccgttgtag
    16981 atcagcacgt gccgctggtc ggcggagatc atcggctggt ggccggccgg ggagaggtcg
    17041 atgatcgcca gccggcggtg gccgaggccg acgcccccct cgatccagtg cccctcgccg
    17101 tccggcccgc gatgcgcgag cgcgtcggtc atccgctgca ggatggccgg cgacgcgggc
    17161 gcgccgttcc gatgcaaaat gcctgcgatg ccgcacatgg tgaaaccccc gtcagcgacg
    17221 aaccgcccgc actgaaaacc cgctctcctc ctcgtggaac cgcgcgatct ccagccccgc
    17281 ctccgcgcac caggcccgca cctcctccgc cgtctgccgg tgcgcgtacc ggggccggta
    17341 ccagtcgaag ttgacgtgca cgttctcctc gaacgacagc ttcgcgttcc agtacatctt
    17401 gaccacgtcg tagtacagca gccgctggat gtcgtacgcg ccggcccgga tgcccagcag
    17461 cggcacgtcc tccggcacgg tcacgctcgc cttcagctcc gccagcgcct gcccgaaccg
    17521 cgtcagcgag cgcatggcct cccaggcctg ctcgtccgtc agcggcgcga tctgcccgcg
    17581 gatgtagtcg tccgcgtact cccgcagcgg ggccttcttg cggtacacgt agaacaggaa
    17641 ctccccgccg cgcgccacca cccgcgccgc cgagagcagc gccgcccgcg tcgagggcgt
    17701 atggtgcagg acgccctccg agaagaccgt gtcgaaagcg ccgtccgcga acgggagctg
    17761 cagggcgtcc gcctgcacga agtgcgtgtt cgcgacatcg cgcagccgct cctgcgccac
    17821 gtccaccgcg ctggagatgt ccgcccccac ccacatcgcc cggcccgacc aggccggatg
    17881 gcggaggtac agcgcggagg aaaacgcgct gccgcagccg acgtcgagaa tgcgcccgcg
    17941 cccggcgaag aaacgcgcca tcgccgcctc gtcgccgaac ccgtacttcc gggaatacca
    18001 ctccgccgcg aaggcccgga actccggcgt gtcgtacgtg tcccgcttcg cccacttgaa
    18061 gccgaacgcg tcccgagtct ggatctgccc ggcgtcctcg gtcagcacga agcggggaat
    18121 cccgttgcgc acgggatagc gtccgcctcc ggccgacgcc aattcgcccg ccgcctcgtc
    18181 ccacgtcagc gggccgcgcg ccacggggtc gcggagcagc gcgcacaggg ccttgttcac
    18241 gtcgcgcctc ccttagaccg ctccgcccgg acctcgcgga tcagccgctc gatcacgatg
    18301 tcctggtcgc tctccgtcag gtcgtagtac agcggcagcg ccagcgcgcg gtcaaacacg
    18361 cgccggctgt cggccaggga gccggcatgc cggcaccagg ccgacggctg gaaggccggg
    18421 tgcatgtgca gggcgtaatt accgacctgc acctcgatcc cgtccgcccg catccgcttc
    18481 atgatgggat tccggtcctc gacgaacacg cagcaggtct gccacgaatg ccgcccgccc
    18541 ggcgtgaccg ccggcagggt gatcccctcg acgccgccca gtttttcccg gtaccgcagc
    18601 gccagcgact cgcgcttcgc gagcagttcc tcgatatgct tcatctgcac cgcgccgacc
    18661 gcggcgagga tgttgctcat cttgaagttc gtgccgatca ttccgaactg gatatcctcg
    18721 cgctcggcca tgcgcatcat gccgaagtgc ttgtaggaat ccatccaggc cgcccagtcg
    18781 tcccggttcg tcgtcaccag cccgccctcg cccgtcgtga tgaacttgcg cgggtggaag
    18841 ctgaagacgc tgatgtccgc ccagttgccg accttcttcc cgttccacga cgcgccgatc
    18901 gagcaggcgg agtcctcgat gatcttgaac tggccgcgcc cctggatctc gcgcaaccga
    18961 ttatagtcca gcgggttgcc gaacattgac accggaatga tcgcctttgt tttcggggtg
    19021 acggccgcct cgagggcgtc gtagtcgatc agcatcgtgg ccggatccac gtccaccagc
    19081 accatcgtcg cgccgacgaa gcgcgcgacg gcggccgtgg cggaataggt gtagtcgggg
    19141 acgatcacct cgtcgccggg gccgacgccg aacaggcgca gggccatctc catgccgacc
    19201 gtgcagttgc acgcggccag cgcgtgggcg cacccgatgt gctgccggca cagcgcctcg
    19261 aactcccgcg tggtggcgcc ctcggtcagc tgcccgccgt cgagcacctg gcagaccttg
    19321 gccttcacct cgtcggtgat gtagggcttg atcaggggga tcttgtgcat ggtcaccttc
    19381 aaaccaggcg gcgccgcgcc tcgacctcgg cgatgtgggc cttgcgccag tcgatcagcg
    19441 acttcaggcc ctcctccagg tcgatcttcc acgtgaagcc gagcttctcg cgcgcggcct
    19501 tcgggcagcc gacccggttc gtcacgaaat attccgtcgc cggcttgaag ttgatgccga
    19561 gcgactgctt gccggtcagc ttcagcagca cctcgatcag ctccttcacc gacgtcttca
    19621 cgccgcgccc gacgttgaag aacccgaccg ggcactccga cgtcatcgcg cacaggttcg
    19681 cccgcgccac gtcctcgacg tggatgaaat cgtaggcctg cgagccgtcc ccgtacacga
    19741 ggggctgctc gttgcggtcg atgcagtcca gggccttcat catcaccgcg atgtacgcgc
    19801 ccttgtagtc ctggcgcggg ccgtagacgt tcatgtagcg caggcccacg cccttgacgc
    19861 cgtagcgctt gtggtaggcc ttcatcatcg cttcgccggc gatcttcgtc gcgccgtaga
    19921 acgtccagca gttgaacggg tggctctccg tcatcggctc ctcgaccgcg tcgccgtaga
    19981 cgctcgcgct ggaggagtag accagccgct ggatgtggtg ccgccggcag gcctcgagca
    20041 cgttgaacgt cccgcggatg ttcacctcga aggccgactg cgggtactcg tggcagtgca
    20101 gcagccacag ggccgccagg tggaacacgc cgtccacgcc ctttatcgcc gtgtccagga
    20161 cgtccggctg cagcacgtcg ccgcccagcg ggaacacgct gcaccgcgga tccttcagcg
    20221 ccctggccag gttctccatc cggccgcgca cgaagttgtc gtacacgacc acttccttga
    20281 cgtcggtctg caggagctgg tccacgatgt ggctgccgat cagccccgct ccgccgatca
    20341 ccatgatctt cttgcccttg atgtccatgt agtcctccgt tgatagtctt cttcgatgtg
    20401 caggcccggg tagggcggct gcgccgaagc cgccgcggcg cgcccggcga gcgcgcccca
    20461 cctcatgccg acaggaattc cttgatgctg tccaccacgt acgccgcctg cgcgtccgcc
    20521 agttcgggat aaaccggcag cgccaccgtc tcgagcgcgg ccttttcgct ttccgggaag
    20581 tcgcccttct tgtgcccaag atacgcgaag cactcctgaa gatgcatggg caccgggtag
    20641 tacacttctg tgccgatccc tttgtccttc aggaagttct gcagttcatc gcgccgcggc
    20701 acgcggagga cgtactggtt gaagatgtgc cggttggcca ccaccctggg cagcgcgacc
    20761 ttgtccgtca accccgcggc ggcgaacagc ttgtcgtatc gcttcgcgtt ggcctgccgg
    20821 cccagcgtcc acgcatcgag gtggcggagc ttgaccagca ccaccgccgc ctggagcgcg
    20881 tcgagccgga agttgccgcc gatgattttg tggtagtact tcggcttcga tccgtgcgcg
    20941 cgcaggatag ccagcttgtc cgcccgcgcc gcgtcgttag tcgtcacgat gccgccgtcg
    21001 ccgaagcagc cgaggttctt cgaggggaag aacgagaagc aaccgtagtg gccgatcgag
    21061 cccgcgcgcc ggcccttgta ctccgacccg atcgcctgcg cggcgtcttc gatcacaacc
    21121 aggccgcggc ggttcgccat ctccatgatc gggtccatgt ccgccatctg cccgtacaaa
    21181 tgcaccggca tgatcgcgcg cgtcttcgcg gtgatcttcg cctcgatccg tgcggggtcg
    21241 atgttgtacg tcgcggggtc gatgtccacg aacaccggcc gcgcgcccgt ccgggcgatc
    21301 gagcccgcgg tggcgaagaa cgtgtagggc gtcgtgatca cctcgtcgcc cggcccgatg
    21361 ccctcggcca tcagcgacac gagcagcgcg tccgtgcccg acgtcacgcc gaccgcgcgg
    21421 gcgcacgagc agtaggccgc aatggccttc tcgcactcct ggaccttcgg cccgaggata
    21481 aaatattgcg actcaacgac ttcgcgcagg acaggctcga tctcggcctt gatcccggcg
    21541 tactgcgctt tgaggtccag caggggtacg ttcatgggtc tcctttagtt ggatggtatg
    21601 aaagtgtgaa ggtgcgaagg tggaaaagag ttaaaaagct gcccgtttac gcaggctgcg
    21661 agtcagggcg gcgattccgc gtgacagcgt tgctgttttg tttcttaagg cctccgcgtt
    21721 ttcggagttc aagtaaccaa ggtcttccgc gacgtaaagc agacttcgca attctccggc
    21781 agaggctttc gcgatatcca ggaagcgcgc gaactcgaca cgggtttttc gctcaaaccc
    21841 ttcggcaata ttactcatcg cggacaatac cgcggcttgt gcctgtgttc tgaattcaag
    21901 gtctcgctgc gcaggcgttc ctttcccaaa gacggaatac acatccacca caagaactct
    21961 ggcctcctgc catattcgca actcctcaaa agacttggct aacgacacgc gcaactcctt
    22021 tcacggcctt gatattgcac ctcttcccac cttcccacct tcccaccttc acactttcac
    22081 actttcccac cttcacacct tcccaccttt gaactcatcg tacgtcttct cgcccttcgc
    22141 cagctccgcc ggcaacgggg cctcctcatc gaggtccagg catttcagca cgccgggctc
    22201 gacctccttg tagcggaagc cgctctcggg gcagcgcatg accccgtcct tgtccggcgc
    22261 gccgagccgg tggccgtgcc ggctcatcca gctcttctgc cgcgcggggt tgcccatcac
    22321 gagcgcgtag tcaggaacgt ccttcgtcac cacggagccc gccgcgacga aggcgtagcg
    22381 gccgacggtg acgccgcaga cgatcgtcgc attggcgccg acgctgcagc cgcgcttgaa
    22441 gcgcgtcttc tcgtagaggg cgtgccggtt gacctgcgaa cgcgggttgg tgacgttcgt
    22501 cagcacgcag gacgggccga gaaacacgtc gtcttcgatc accgtgccgg tgtagaccga
    22561 gacgttgttc tggatcttga cgttgttccc catcaccacg ccgtccgcga tgtggacgtt
    22621 ctggcccagg atgcactttt tgccgatctt cacccgcggc aggacgtggc tgaagtgcca
    22681 gatcttcgtc cccgccccga tctcgcaggg ctggtccacg caggccgtct cgtggacgaa
    22741 gtactcgcga gcgggcccgg acgcggcggc cggcgccacc gcgccgccgc cggaggccag
    22801 cgaccgctgg caggcgtcca ggaccgtcag cacgcgcagc ccctcggccc cgtccgtcac
    22861 cggcgcggcg cgggaatcga ggcaggcgat gaagtggcgg cactcctcct tcagcggctc
    22921 ggacgcgtcg agcggtacca cctcggcctc ggccttgacc gccgtcggca cgcggtcctt
    22981 ccactcgacc ttgtgcgggt agaggaccag cttgttcttg gccgtgtcgt cgaacaccgc
    23041 catcttctgc gagccgacga cgaccaggcg ctgctccttg aaggggtgca gccagttgac
    23101 gaagatgtgg gcgcgcacgc cgctggggaa cgcgaactcg ctcgtcgtca cgtccatcac
    23161 cagcgggttc aggtagccgc cgccctggca ggccacggac atgggcattt cgcccagcag
    23221 cgcaagcatc acggagatgt cgtggggcgc gaagctccag aggatgtttt cctccgtgcg
    23281 gatcttgccg atgttcagcc ggttggagta gaggtactgg atctggccga ggtcgcccgt
    23341 cgagatcagg cggcccagcg caatcacggc gggatgaaag cggagaatgt ggccgaccat
    23401 cagaacgcgc ccgagccggc ccgcgaggtc caccagcgcg cggccttcct ccaccttgat
    23461 ggcgagcggc ttctccacga acacgtcctt gccggcctcc atcgccgtgc gggccatccc
    23521 gtaatgcgtc accgccggcg tggcgagggc cacggcgcgg atgccgggat cggccaggac
    23581 gcggccgaaa tcccgcgtga accgcacgtc cgggaacttg gccttccact ccttctccac
    23641 tgccgggctg gtgtcgcaga tcgtgtgcag cgcgcccagc tcgtggaagt tacgggccag
    23701 gttcttgccc cagtacccca tgccgaccac cgcgatgtcc ttaatcatgg catctccctt
    23761 ggaccaggag gcgcaaagga cgccaagcct gatcttctct gcgaaccttg cgccttctgt
    23821 tcaacgatct catttcagca gcatcttcaa aatcgagctg tcccacaacc gctgggcgcg
    23881 gccgcggaac gcgcggatct tcgcaacccg gtcgcgcaga aaggtccagt cggtcgcgtc
    23941 gtccgctgaa ccggccgcgg cctcctgcat ctcctcctcg gcatcctgga gaacgtggtc
    24001 gccggactcc atcttccggc cgatcacgtc cagcagcgcc cggcggatga tccgcccgaa
    24061 atggtcgagg gcctcgtagt agtccttgcg ctcgcccttg atccagacct tgcgcagcgc
    24121 gccccactgt tcgagctggc ggaccgtcat gcttgcgctg cccttgctga tgccgaggga
    24181 ctgcgtgagg tcgtccaacg actgcggctg gcggctgaag tagatgtggg tgaagacctg
    24241 gcccagcgcg cgcccgacgc cgagggtctg ggtcatctgc cccgccgcct cgatgaagcg
    24301 ccggcgcatc tgctgtggag tggagccgct catatttcaa caacgaactg aaggcgcaaa
    24361 gaacgcaaag gaaggaatca ccacggagga cacggaggtt cgcggagtag aaaaccgatg
    24421 gggaagcggg tgaacgcatc aggagcctgc cccctctgcg ctcctctgcg ccctctgcgg
    24481 ttcaacttct gggccatctt ggcgcccctt gcgccttctt tttttccggt cccggcggac
    24541 ttcgtccccg tcacgtcccg gggcgaacgt tcaatttgta ttgaacgtat caaaaaggag
    24601 ggtgagcgca agcgttattt ttatccgcac ggaggcggcc ttgaacgccg ttattcggcg
    24661 cgccggaacg gcacgaggag gtccgactgc gccgcgacat cggtgcggat ccgcaggtag
    24721 gcgtcgccgg acaccacggc gcccgggacc ggccggagtg tgatcgcata ggccgccggc
    24781 ccctcccccg agatggaagt ctccagggac ggcggagcga tggcctcctg caccgcgaag
    24841 gcccggaacg gtgactgcac gaccacccgc agttcgcccg tgatcacgcc gtcggccggg
    24901 accaccagtt cgcggggctc gacacgaagc ggcagttcca ccgccgccga aacgggaatc
    24961 tcgaggtccc gctgcatggg gtcgtccgtc agcacccgca cggtcccgct caacgcgccg
    25021 acgggaagcg gcggcacggt gctcacggcg atccggtagc ccggcccgga ctcgttgggc
    25081 cccgccgcgg cgcggaccgc agccgaggaa ctctgcgcct cgtgcagttt aaggggccag
    25141 ctggtgttga acgtgagcac gacggactgg gtcacagcgg cgtccacggg gatcgtcccg
    25201 aaggccacgc gctcgggctc caactgcagg gcctgccgcg cctcgccccg gaaacgcaag
    25261 gtcagggcct gcccggggaa atcgggcagt tccacggcga aatccttggt cacttccccc
    25321 ttcaggcccc gcggcgaaaa gccgaggttg acctgggctc tcgagtccgg cgccagcacc
    25381 aactcccgcg ccaccgtcgg cgccgggtcc ggatccatga tccgcacggt caacgcctgg
    25441 ttggtccggt tttcgaggac gaagacatgg tcgacccgcg tgcggttgtc cgcccagccg
    25501 aagaagtact ccggcgccgc gcagcccaag ggccccgccg aagtggcgag ggattcggcg
    25561 gcaacaggac gaaccgccag gaccgcgagc agcagaaacc gcgaaatgat gggcgccagt
    25621 ttcattacac gaaacccgga cgggccgtcc cggggcgcgc gggcgccccg ggatccttcg
    25681 ccgccaagct acggcgcgat ttccgcctgg acccggtaga agtacggcgt cgggttcgtg
    25741 gcggtggcgt cgtggtaggt gttcaccggc ggatcggcct cgatgttgct gtccagttcc
    25801 gagaacccgg cgaccaggtc ggtcgcccga tccaattggt agacgcggcc cgtgagcgac
    25861 ggccaagtaa cctggaggcc gacggccggc acgccggcgg ggctgacttt ccagaacgcc
    25921 tccgggttgt tcggatcaat gccggcaatg taggtctcca tcaccgtgtt gaccccgtta
    25981 gcggccatgg ccccggcgtt ggcgttggtc ggaccgagga aatgctccgt ctcccagtca
    26041 ttgggaatcc cgtcgccgtc gatgtccggg tcgtcggggt tgccctcgcg ggtgatggtg
    26101 accgagtcgc tgatcggcgt ggtgccggtt gacgcgcggg tcacccgcat gtcgcccagg
    26161 tagaggttat actgccagcc ggagccggac cgccagttcc aggacttgaa cttgctgatg
    26221 gacgttcccg cgtgggccag cgtgcccgtc aggatggtcg cccccacggt cagccggtac
    26281 gcgtcggcgc cggtcagggc gaacgtgatg ttcagcccgt cgccggtcca cggaatgccg
    26341 gtatgccggc tggacgtgct gtcgttgatc acgtagttgg ttccgccgcc gatgaacatg
    26401 aactcgaacc ggttcccgtt ggtcgagttc tgcagggcga agcccacgga gccctgggga
    26461 tcatcgtcga cccagttgtt gtcccagcgc aggctgaacg aatcgcccgc ggccagagcg
    26521 ctgttgaagt tccggatcgc atccgaggcc gagtccgtgt gcgcccacat gccccagccc
    26581 gcggcgtcca cgctcatgtt ggtcccgtcg gccaaggtgg ctcggaactg gcccgcattg
    26641 gcgccggcgg tcagcgccca ggccccgagg cccgagccgc cgttcgagtt gttcgtccag
    26701 ccactgttgt aggacgcgtt ggtcgggttg tcccaagcca cggtttcctg cgcggaggag
    26761 gtgtaggcgc cgctgacggc gatcacgttg gcgccggtgg ccagcgggat atccgcgacg
    26821 ctccacgagg ccgccgtcgc gaggacgccc gtggcgccgg tggcctggtt ggtccagctc
    26881 aactggccgg ccaggttgcc gtaggccttg cccttgagcg tgtaggtggt caccccgttg
    26941 gccacttgcg tgtccgtcgg cgggttggtg atggccaggt cgtagcagtt cacataagcc
    27001 cagtaatcgg ccgtgttgtt gttggcccag aactcgttgg tggccgcgtc aaacgccacc
    27061 acggcgtagc ggacggtcga gcaggccggg taggcgccga ggttgcaggt ccaccagttg
    27121 cggttcaggg cctggccggc cgcgccgccg tacgtcaggc ccttgttggt ccagttcccg
    27181 ccgttcacct ggtacgccac ggcgccggtg accgccagga acagcggcgc tgtttcgaca
    27241 tacaccacga tcccgtcgct cggcgtgatg tagccgttcc ccggcgtgtg gttgacgttg
    27301 gacagcgtga ccgtcgggca gttcacgtag gcgaagtagt cgttgtcgtt gttgctgtcc
    27361 cactcctcgt tggtgttcgc gtcgcgcatg acgatggcat actggatggt cttgcacgcg
    27421 ccgaacgcgc ccaggttgca ggtccaggaa tcgttctggc cgaccacggc gcccttgacc
    27481 agggccttgt tggtccaggt ggttccgccg ttggacgtgt agaccacgtc gccggacacg
    27541 gccgcgttgg tgggatacga ctcggtgtaa accgtgatgc tggaactcga ctcgatggcc
    27601 ccgttcgtgg ggctgtgcac cgtgttgccc acccacgcga cggaggacgt gtcgccgccg
    27661 ccaccgcccc cgtgctcctc gagcttgagc atctggacga aggccgtctc cgggttgccg
    27721 ttgccgtcgg ccggcatgtt gatatagatg ccgctgttga tcagttcctg gcccgtcttg
    27781 cccggcgcgg cccagaggta ggcgtcggga tcgtcggcgc agaggttctt gatgttgtaa
    27841 tagcgcgtgg ggttcaggta gatcgccgtc acctcggcga ggttgaacgt gccggactgg
    27901 ccggtccacg gagtcaggtt gacgaacgag agcaccacgt tctggtgggc cgggtcccag
    27961 ccgtacttgc tgccctcgta cttcagcacg gagaagatct gctcgtgggt gcctgccccg
    28021 ccggtgcggt tgaggtacca ctggtcgtgc aggcggctgg cgatgttgcg ctggcggccc
    28081 ttgttgacgc ggctgtaggc gccctgcagg acgttgtcgc ggttatgcca cagcgtcagc
    28141 atgttgtgcc agatcaggat gttcggaatc caccggtcga actggttgcg gaactgcgag
    28201 aagccccagt ggtccgtgta cccgagttcc tggccctggt acatctgcgg cgccccatcc
    28261 accaccgcgc ccaccgcgta gcgcgagaac gtgtaccact tgtccgccgg cgcgctctgg
    28321 tcgtggttga tcaggccgcg catgatgccc gcgtacccgt agtcggtctt ggtgccgtcg
    28381 atgacgccgc gcatgccgct cgtgttgccg ccgccgccca gcacctgcca gacccagttc
    28441 tggttgatga tgtccacgtg ccgcccggcg cgcttggaga cgttgccgcc gtccagcgac
    28501 tccgccatga agataaagtc ccacttgctc tggcgcgtct tgttgatgat gtactcccag
    28561 gcctgcgggg gcaggccctg cgcgtagtcg cagcggaagc cgcccagcgc gccgcccgac
    28621 ttctcgatcc agtacttccc gaagtacgcg aagtactcga ccatgcgctt cacgtccgcg
    28681 cccgtggcgt tgggatccca gtagctgtcg gtgtcggtct gcggattgga cagcgcggga
    28741 taggtgcccc agtagaggtc cgccacatcc ggccacttcc cgaaatcgct gcgctcggcc
    28801 ggagcggggc cgatctggct cgaggatgtc gccggcagcc agtactggaa gaggctctgg
    28861 ttgtaggaat actcgccgca cggcaggtcg gtggacacgt agcgcgagta ccactgcggc
    28921 aaggtgtcct tgatcttggc cgacgggttg cccgccgggt tgttcggatt ggccgggtcg
    28981 cgcccgatct cggcgtccca cgaggtgtgg ttgaagatcg tgtcgaagaa gaaatccagg
    29041 ccctgggcct tggcggccgc cgcgaagttg gagaacgcca ccatcgaggc ggcgcgcgtg
    29101 ttgccgcggc tgtggtgcgg cgccacctcc cacatgttct ggatcgagta cgggctgccc
    29161 gggtcgctgg acacgcagta gtggtcgccg atgggatgga tcggctggat ccagagcgtg
    29221 ttcacgccga tcgaggtgaa gtgattcagg ttggcgtagt tcgtcgggtt caggaagtcc
    29281 tcgaacgtgc tgcgcgtgcc ttcggaatcg ccggacgcgt tgatgacgtt cgcctgcagt
    29341 tcgtagagga tgacgtcgcg ggccttcttc ggggagacca cgacggccgt atcgcggacg
    29401 ccggcccagc gccagttcgc gtcgccggag accttgtagc ggcagttcag gcggtaggcg
    29461 ccggtcttgt tcgcaggcag cgacgcgtac cacttgccgc cctcgcccgc cgtcatggtg
    29521 gtcgcgacga agtagttcgt gccggcggga tcgggcccgg gatcgctcgt cgcgtcctgg
    29581 tcgcccagcg tcgcgcggtc gcggcggctg acgttgtgcc agatctgcgc cttggtgacg
    29641 ttggcttcgt tcgggtagaa atcgacgtac accgtctcgg aatccgtcgg ctcgtcgatg
    29701 tagtaatgca ccgtcaggta gtcgccgttg gcgccgttga ccgacatgta tggcgccgcc
    29761 caggcgctcg cggcgacgag catcaccgcc gccatgaccc acggaattcg gatcgtgctt
    29821 ctgctcattg cggtctcctt gattataaga tcgtgtttaa gttaaccgtt ttaccaaaat
    29881 ctgtatagag ctttttccga gcagggcggc ttggctcgct tgtccgccga agcgtaacgc
    29941 gcgaaggcgg aagccgccga tcccggcgcg tttggccaac gcgccctacc tatttatctg
    30001 cactatatta cggcgacagc aaccggaccc tgtaatgcct ggccccgctc gccggcagcg
    30061 gcaaattatg cgtggccgac cggttggtct ccacgccatc cgccgccgtc tcggtcactt
    30121 cctggaattg cacgaacccg ccttccggat ccagcaggtt cgtcgacacc tgcaaactat
    30181 atacccgtcc gcccacgctg atccagttga tctgcggata ctggccgttc agcacaacgc
    30241 cgttgcgaag gaccagcccg gaggatttct cctccggatc cgtacccaga cggtactcct
    30301 gctcgttggt gaagccgtcc tcgtccgggt ccgcgccggg cgcggcgtcc ggatcgcccg
    30361 cggagcccca gtgctcgatc tgccaccagt ccggcaggcc gtcaccgttg ccgtccgacg
    30421 cgcgcacgat ccgcaccgtg tcgccggtga cgaccacgag ggactggacc tcctgcacgc
    30481 gcaggtcatt gacgtagaag ttgcggccct cgttgttctc gccgttgacg ccggaattat
    30541 aattccacac gcgcacgcgc cggatcgcgc tgtccgcctg gctgatgagc gtcccggtga
    30601 acgtgctcgt cgcgctgccg tacgggtgca cggtcagcac gtagcccgtc gtgttggtca
    30661 tccgcagcgt gatgctcaag ccgttactcg tccacccgag gcccgtgttc cggtcgcccg
    30721 tgctgtcggc cagggtgtag ttggcctgcc cgccggcgaa caggaaccgc agcaggtcgt
    30781 tggtagccgc gttctggatg gcgacgccca cgctggagcc gtacgtcccc gtgccgtcca
    30841 cgccgccgtt ctggaacttg aacgagaggg agtcgcccac cgcgacggcg ttgctgaacg
    30901 cccgggagcc ctcggccatc tgctggctgt tggcccacag gccccacgcg tagttgccga
    30961 tcgtcagcgt cgggttgccg gtcttcgtcg cccggaaatg gcccgcgttc gcgccctcgg
    31021 cgtagagcgt ccacgcgccg aagcccgtgc ccgcgttgtc gccgctgtcc caggccggat
    31081 cgtaggccga cgcgtccgcc gcgtcggagg ccttcgtgac cacctgcccg gacgtgttgc
    31141 tgccgaccac ggtgatcagg ttcgtgccgt cgccgacggg gatggaggcg atggtccaac
    31201 tggacgcgac ggccagcgag cccgtgccgc ccgtgagggc gttggtccac ttcaggtgcc
    31261 cggcgatccc cgcgcccgcc gtgccgccga tcgtggcgac ggacgtggtg accgtgagcg
    31321 agtcgccggc cgggttggtg atcacgacgc cctccgcgag gccggcgcag ccgctgacgt
    31381 tgaagtgcca gtccgcgccg tcatggttgt cccagacgcc cgagtcgccg ccgccgtcgt
    31441 tgaacaccac gtcgatctgg taggtccctg ccggcggcgt gtacgtgtag acccacgtgt
    31501 tggcgttgga ctgggtcatg gccgggttcg gcaggatcac gccctgccac ccgttgtagc
    31561 cgacgtgaac gtgcacgggg gacacgtcct tcaactcgcc gccgttggga tggtagacga
    31621 tgcgaacggg gtcgcacccg gtcgggctgg gcggggcggt ctcggcggcg ccggggcccg
    31681 tcggttcgtc gcagtcgcgg atcgccgtgt accagttgag cccgttgttg ctgtccacca
    31741 ccgtgccggc cgagttctcg aaccagacca gcgcgctcgg cgcgttgtcc ggcgccgtgt
    31801 tcgtgcacac ccacaggttg ttgccggcgc tggtcatcag atagcgggcc cagttcgtgc
    31861 cgctgtcgaa gctgatctgc tggtagaccg ggctcacgcc ctgcaggacg ccgcccgtcg
    31921 cgctgaaggt caccgccagc ggcgcgcaat cgcgcgggtc gtcggagaac gtggcgtagg
    31981 acgccacgcc gcccccgccg tcgtcctcga cgtagacctg ctggatgtcc gtccggttgg
    32041 tgttgccgcg cgtgtccacg gcctcgatgt agtagtccag cagcttgccg cggaagttcg
    32101 ggacgctggc cgaggtgatc ttggcgaagt agtaatccgc gatgtagggg gcggttacga
    32161 aatagtcgat ctgcccgttc gccgccgcgg cgttgagcgc gctccgcgtg ttcggcagga
    32221 cgcgcttggt catcggcacg ctgatccacg agccgacgtc cgagccgccc gcgtacgtct
    32281 cgttctggtt gttcgccatc gagttcgcgc cgtcgttgtc cagccggacc ttggcgacga
    32341 tgctcgtgat gccggagacg tcgtaggcgt gcgtccagat gtagaactcc gagcccatct
    32401 tcttgaggaa gcgggtgtcg ctcgggatgt tgttgaacca gccgaaggtg taccagcccg
    32461 ggttgtacgg gaagcgctgc ggcttgagca cggtcggcgc ggtgcggtcg ttcgcgatcc
    32521 gcgcgttgac ccaggtctgc agcagcccga tcgcgcgcgc cgtcgccagc gactgcttga
    32581 cctcgtcgtc gttgcccagc ccgccgtagt agttgaagcc ggagtccagc ccgccgaggt
    32641 agacgtgcca cgcgcgctcg acgaggttcg gcgaggtgta cgagccgtcc cagtcgtaga
    32701 tgcactggat cttccacggc tggacgttcg tcccctcgtc cgccaggatc tgctccgccg
    32761 tctcgcacca gttcgcgccc gacatcaccg gcgcccagct ccagaacttc agcgcgaagc
    32821 ccggcgtctc caggtcgatc ttcgtgcccg ggtagcaggc cggcaggttc gtcgcgttca
    32881 ccggcggctc tatccatttc aggaagtacg gcgacccgta gtccatctcc gggaagatcc
    32941 acgcgccgtc ctcgatgtgc accgtgtccg ccgagttgct gaacgccttg aagaagtcct
    33001 gcggggtgct ctggtgatag cccgcgctgc cggagtcgtt gaagaactgc ggcgtcgcct
    33061 ccatccagga ggaattcccg ccgccccacg cgttgtcccc gtccgtcgcc ggcatgacca
    33121 tcaccgggtg gctcgggtcg gtggcgaaag gcgagatgta ggtttggatc ttcgagatcc
    33181 cttcggacgc atatccatag cggtagctca acacgtcgtc ggagggcacg accaggatgc
    33241 tcttctccgc gccggtcgcc gggtccacgt acttgacctt gtgcagctgg taggcgaacg
    33301 gggacacgtt ccaggcggcg ttgccggggt tcggctcgct gtaccaccag ccggatgacg
    33361 gcgacgggcc gagccggtcg gcgcggttcg gcgggctgga aaagatgttc cacgagcccg
    33421 tcgggttggc gctgttcagg tacgtcgggc acgtgcggct caagtggtgg ctcgcgacga
    33481 tgctccactg gtagccctcg tccgccagca cgtcgatcat gtggtgcgag aaagccatct
    33541 cggtcgggaa gtagcccttc gagtggtcgc tcaagtcggc gttgccgccc cacgccttcc
    33601 accacgcctg cttgaagacc tgcagctcct tgcggaagac cgccttcggc agcagcggcg
    33661 cgagggagtg gtggtagttg aagccgacca ggtcgagccg gcgcgcccct gacggcgtgg
    33721 tccagttccg cgcctgccgg ttgccgtccc accagccgct gccgtagccg agctgcccgt
    33781 tcgcgccaag gttgcgcacg ttgtcgatca gcgaacccga gtagctgatc gcgaacccgc
    33841 cggcgctgtt gatgttggcc agcgaatcgc gcggccggcc ctggtaggcc gccttccggt
    33901 catccacccc gaagatgtca gacaggttgt tctccggatg ccccacgctg gtgtcgtatt
    33961 tctggccgtc cttgagcacg atcgagtccc acgcgtactg gatgcgctcg gtctgcgagc
    34021 cgttgccgtt ccactcgggc cagtagatcg gctggtggtt gtgccagaaa cggctgacgt
    34081 agacattgcc gttcccgagg gccggcgcgg ccacggccag taaaagaacc cagcacacaa
    34141 tcgtctgctt cgcggcgcgc atgctcttca ttgtacctcc ggcggcaaca aggtaaacgg
    34201 acggccagaa agttctatcc ccggctacac aagtaaagcg ctacaccgaa gccgggaggt
    34261 tgttaagagc cggattcgcc caaccgcttc tttacactga aacccccgcg accctgcgtc
    34321 gcgggtgaac gaggttgggg aaaagcggcg aaggtcgccg cttttccctg gccgcgcgct
    34381 cctgccaggc cagtcggcag ggggcgcagc atgctaacat attaatatac agataattat
    34441 atccgctccg gcgtctcgat gccgagcagg gacaggcctt ggcggagggt cttcatggtc
    34501 agttcgcaga gccggacgcg gctttcgcgc gggcccgcct cggccttgag gaacgggacg
    34561 ttctggtaga acgtgctgta ggtctgcgcg aggtcgtaga ggtagtcggc cagcaggttc
    34621 ggcttgaagg cctcggccgc gtggaccacg gcctccggga accgcagcag gcgcacggcc
    34681 agccggcgct cgatcggctc ggtcagccgg atcggatgcg cggccgggtc ctggcccggg
    34741 aaccgctcgc ggtacttgtc cagcacgctg gcgatccgcg cgcaggcgta ctgcaggtac
    34801 ggcgccgagt tgccgtccag cgcgagcgcc ttctcccacg tgaaggtcac gaggctctgc
    34861 gggttctggc tcaagtccgc gtactttacc gcgccgatcc cgaccgcgcg ggcgaccgcc
    34921 ccctgctgct ccgccggcat ctccgggctg gccgccttga ccaccgccag cgcgcgcgtc
    34981 tgcgcctcgt ccagcagccg ctcgagcttg atcacgttgc cctgccgcgt ggagaacgtc
    35041 ccctccggca gccgcatcag cccgaaccag acgtgcacca gccgcgttgc cacgcccatt
    35101 tttttcgcga tggcaaagat ctgccggaaa tgaagctgct ggcgctcgtc cgtcacgtag
    35161 atgaccgcat cgggcgcgaa ttcctccgcg cgcgcggcga ccgtggcgag gtccgtcgtg
    35221 gcatagttgt aggccccgtc gctcttccgg acgatgcaca ccggcatttt ttcatcgtcc
    35281 agaaacacga ccagcgcgcc ctcgctctcg cgggccaggg ccttctcttc caaccgctcg
    35341 atgacgcccg gcaaccggtc gttgtaatgg ctctcgccgc gcacgaggtc gaaggacacg
    35401 cccagccgct cgtagatccg gttgaactcg cgcaggctgt gctcgacgaa cgattgccag
    35461 agcctccggt tctcggggtc gccctgctgg agcttcacca gttccgcgcg cgccgcgtcg
    35521 agccacgcgg gatcctcctt cgaccgctcg tagcttttca cgtagatccg ctcgagctcc
    35581 tcgatcggcg cggtttcgta ggccgccggg tcggcgaaat gccggaagcc catgatcaac
    35641 agcccgaact gcgtgcccca gtcgccgaga tggttgtcgg cgatcacccg ccagccgagg
    35701 aaccggtgca ggcggtcgag cgcgttgccg atgaccgtgg accggatgtg cccgatatgc
    35761 atcggcttgg ccacgttggg gctggagtaa tcgatcacga ccgtccgccc gcggccgggc
    35821 ccgggaacgc cgaggcgggg gtcgtcgccg agttgctcca ggcgcccggc catccacgcc
    35881 gggtcgagga acaggttcac gaagccgggg cccgccacct cggccttcgc tacggcttcc
    35941 ggccgggcgg cggcggccag cgcgctggcg atttcgcgcg gcggcttctt cagcgccttg
    36001 gccagcgaca tggccgcgtt gcactggtag tcgccgaacc gctcgtcggc ggtcggcacc
    36061 acgccgacgg acaggccgtc cgggatcgag tccgggaagg ccgcctggaa ggcgccccgc
    36121 gcccaatccg acagcacctg ctcgatggtc aggacatcat tttgcatcgg gtcacctgtt
    36181 gccgttccga atgcctcatc agaactgcga atccatcaaa aggttgcgca ttccagagaa
    36241 aaaaacgttt gattccttct tgcattcctt tttcatcgcg ctaaagtccg ccgcgaatcc
    36301 ggtatcaaac ccttaaaaat caggaggagt aaaatggcac cggcgaagaa gaaaagcgag
    36361 aaagaagcga ccccgaaggc ccccaaggcc cggaaagcag cggcaccggc ggccgcggcc
    36421 ccgcgcaagc gcgcggcggc gaagcccgca ctcccggccg tcgcggcgga agagcggcag
    36481 aagctggtcg aactggaagc ctatttcgtg gcggaaaagg acggcttccg cggcgattcc
    36541 aagaattact ggatcaccgc cgaggcgatc gtggaagccc ggttggccgg gaagaaataa
    36601 gcaagctgac cgcgtttgga atggaaacgg ccccgattcg tcggggccgt ttttttgttc
    36661 ccctccctta agtcccgtcg tgaatggtcc attacccgtt cgagctaagt gtgttacaac
    36721 aaggtttctg ctttgtgaaa aaagtgttgc acacgccatg ctgaaacgtg taaggtattt
    36781 agctatgaag tgacgcggga ggagagcgct gcttccattc gccaagcaag gaaaggatag
    36841 gcttgtcatg aagaatccgt tcggacgtgc gagcattctg atttgtcttg cgtcggtgtg
    36901 gctgggcctt gcaccggctc acgccggggg cctgccggtg attccgttca tccccatcat
    36961 cccgatgacc tacagcaacg tggaggcccc actccaatac gtgcaataca cgccgcgcga
    37021 gacccgaaca tggacatttc gccatcccgg cgctatcgcc atgcgcatcc ggtttgcggc
    37081 catcggattc tccacgccgg aggagggcgg ctatcccgaa aagcggtcga cgatcgagat
    37141 tctcaacagc cagggcgccg tggtccagac cctcacggcc gacgccaacg catcgttcta
    37201 tagcgcgtcc gttcccggag acgtgatcca ggtgcgctac accaacaagt ccaacggcgg
    37261 gctgggccag tcccctgcgc aggccgcccc ggacaccttc agcatcctcg gctattccta
    37321 tctccgcctg ctcgccgcca aggaagtcgg cggcgcgccg ctgaactact ccgtcttcac
    37381 cccgacggcc ggaacggcct acaacgtcag cctgcacaac cagttccagg tgccggtcac
    37441 ggtcaagaag ccgtacacct tcaacgacta ctcgacgacc aaggtggacc tgttcctgaa
    37501 cggccagttc ctgggaagct cgatgggcta tcgcccggcc tgggatccct ggaacatcta
    37561 cttcagcgcc caactggaca tgcagcagat gatccaactc gccgcgggca actcgacggc
    37621 gacgacggcc agcgcctcca cgctgatcat ccgggaaacc cggtataacg ggaccgacac
    37681 gaacgcgacg tggaagaccc agtaccagta caagaacttc ccgttgacct tctccgatcc
    37741 ccgcgccacg caccatggca acatcaccgg acagggtgat acgggcgtcg gcaatttcga
    37801 ccggctggtg atccacctgc cgtcgcgaag catggtttcc gcctgggttt tttcccagtc
    37861 cggcggcggc agcccgttcg tggactgcga tttcaaggtc atggacgcca acggcgtgac
    37921 cctctacgag ggtaacgagg cccaggtgga cgggcacggc gagggcgtca gcctgatgct
    37981 gggcccgggc gcctacatct tcgacctgtg gcgcaactcg agctcctgcg tggggtacgc
    38041 cgggggcacg gggcacatgt acatcaagtg cgatccggcc gcagcggtga ccgaccactg
    38101 atcagaaagg cgcggcggtt ccgatcttgc cgccgagggc ggcctcggcg atagagcgcc
    38161 cgtccgccag caccggggcg cggcgcggac gccaggggcg acggcgcgcg cgcacgatca
    38221 ggcgatagcc cttggtgagc cccagcaggg tgtccatgcc ggcggcgaac tggaagacgg
    38281 ggtagagtcg cgcgcgccgg aggacgagct tctccaggga tccggcatcc gcgcccgcgg
    38341 cgagcgagag gggccgttcg ccccgccacg ccagcagatc cagcgcctcg acgaagaacc
    38401 ggccgtacga ccgggcctcc tgcacgtcga agccgtcctt gagcaggtcg aagaggacgg
    38461 actcggtgta gccgggccgc acgcggccca gggcctcatc ggtcatgccc agcaggcggc
    38521 gcgcgggccg gaggagggcg cgtttcttga ggtggggcgc gtggacgaga agccggccgg
    38581 cgggcttgag tacgcggtgg cactcggcga tgagcagggc gtcatccggc atgcgctcga
    38641 gctggtcgag gatcacgacg ccgtcgaaca ggctgtcgcc aaacggcagc cgcccctccc
    38701 ggatccgttc gacccgttca cccacgagcc ggagggtcga ggcggcggag gcgtcgtccg
    38761 gcgtcgcggt atgccaggtt ccgcccaggc ggcggagctg cccgctgctc acgccgtcgc
    38821 cgctcacgtc aagccaggtc tggttgaccg ggacgcggcc gaccatcgag gcgatctcgg
    38881 ccagccgcgc ttgcaggggg agcgatttct cgaagagctt gaggtgccag tcgtccggcg
    38941 ccggcgcttc gggttctggc gttggatcgc ccatgccgtc ttttataccg ggccggaccg
    39001 ccgtggtcca gccggctttc ccgcgcccgt cttgcccggc ggtcactccg tctccacccg
    39061 gtacagccac ggtccggcgc cggcggccgc ctggtcggta tgcacgttca gcgggggcgt
    39121 ggcgggcaga ttagtgaaca caccctcgaa aaccccttgc ggatgggtgg agcgggaaag
    39181 gtcgtactgc ttgccggcga cgctggacca ccggatcacc cgccccgaac cctgaaccct
    39241 caaccccgag atctcaaaca ccgacagggc gtcgagcgga tcggtgccgg ccacgagttc
    39301 ctcgccgtcg ttcgcccggt ccccgtccgt gtcggcccga atcgggctgg tgccgatcgc
    39361 cagttcattg gtgtcgagca gcccgtcgcc gtcgctatcg gccatcgggt tcgtccgctc
    39421 gtaacagccc atgtcccagc cggcaggccc gtcgttgttg ccatccagcg ggcggggtac
    39481 accctcgagg tcgcaagcca ccgccggcag gttggtcccc gtgtcgatac agggcgatcc
    39541 gacctggagc tggtaatccc cgttgccgga atccatgaac aggggcgcgg cgttggtgtt
    39601 gccgacgccc gccggctgcg gggccgcgca ggagtggtcg aacgtgatcg tgccgtagta
    39661 gttggtgtcg ctgactgaca ctatgttgtc catgacgatc gagttcttca ccgccacgct
    39721 gccctcggag taaattccgc caccccaccg gcccgtgttg ctgcagatcg tgcagttctg
    39781 caccgtgaag ctccatccgt agatcccgcc gccccgccca ttcgcggcga ccatgccttg
    39841 gttaccgtac agcaaacaat tgcggatcgt ggcattgaag gcatagatgc cgccgccctt
    39901 ggccgcgctg ccgccgccgg cgcggttgga cacaaccacg cactgctgca gcgtgccggc
    39961 ggacatgtac accccgccgc cttcgctgtt gttggccgcc gagctgtatc cgttgcggat
    40021 cgtgaagcct tcgaccaccg cgctggcgtg cgtgattttc acgcacggcg ccgcgccgcc
    40081 gccgtccagg atcgtgccgg ccgggccgtt cacaccgcgc agcgtgacgg gattggtcac
    40141 catgattcgc gccgccacgc gatagacgcc gtcggtgacc agcaccacgg tgcccggcag
    40201 ggccacgttc agcgcggcct ggatgttggt ggcggccgtg ccccacgtgg cgaagggcga
    40261 ggcgtgcgcc ccgccgggcg ccacgtaggt caccgggggt ttcacgttca cgtagttcgt
    40321 cttgcacagc gtcgccgtct cgcccgcggc gttgctgacg ctcaacgaca ccgcgtagag
    40381 gccgaagttg gagtacacgt gcgtgaccac ggacaacccg aggccctggt agtcgggcgt
    40441 gccgtcgccg tccacgtccc aaacgtaatt cagcgcggcg gtgttcgtgc ccgacacgtg
    40501 tccggtcaac gtcacgggca agggcagaaa tccatcccgc gtgccggcga ccggattgca
    40561 ggtcagcgga ccgaattgga attcgtacgc gccgcggtcg ggctgaccgt ggctgacgcg
    40621 cgggttaccg gccacatcct gggcgccgga catccaggtt tcgagcagcg cgttgtccag
    40681 gcaggaggag cccggcagga gctgcaggtt gccgttggcc aggtccgcga actcgggatc
    40741 gccggtcgtg ttgttcgtgc cggaggggag cgggatagcg cagctgtata cgaaggttcc
    40801 ccccgtggtg ttcgtgccgg ccggcgcctg gttgtggcac acgatgcagt tggtgaacgt
    40861 cgccccgacg ccgtaggccc cgccgccgtt gttcgacgcc gtgttgccgg ccacggtgca
    40921 cgcgtccacc acggaattgt tgtccacgta cagaccaccg ccgaaactgg aggcgaggtt
    40981 gccccggatc gcggaattgc gcaccagggc gccgctgccg tacacgcccc cgccgtgggt
    41041 cgcccggttg ctcacgacgg tggaatcccg caggacgccg ccgtaaaaca tgcggacgcc
    41101 gccgccgacc ccggcggcgt tggtgttggc gaagatggcg cagttggaaa ccgtcccgcc
    41161 gcggaagaga taaagcccgc cacccccgtt gctgcagatc acgctcgatt ccacgacgcc
    41221 gcccatgtcc aggtacgccc cgccgccctc ggtcgtcgcg acgttgccgg ccagcacgca
    41281 gcggcgcagc acgccgcctt ccatgtacac gccagcgccc tggggcgcct tgccgccccg
    41341 gatcaataat ccttccaacg ccgcctgcgg atgccggagg tcgaagcagc gcgccaggcc
    41401 ccggccatcc acgatcgccg ccgcctcggc ctcggcccgg atcgttatgc cgtcggtcac
    41461 gacgaccggc tcgttcagtt catacaaacc gtttgttaca cgcaccaggg tcccgccgat
    41521 ccccgtggac acggcggcct gcagattcgt tgcggcatcc acccagttcg tgtacgggaa
    41581 attgtgcagg ccgttggtgg ccgcgaagac cgtggccggc ccgatcttca ggtagtcggg
    41641 cttcagcacc acagcggttt cgccggccgc attgctcacg gtcaacaggg gcgagaaata
    41701 gccgcaggcc gcaaacccgt tggtgaccag ggctagcccg agcccctggg catccaccac
    41761 gccgtcgttg ttgaaatccc actggtaata aaggcccgcc gtatcggcgc cgtccacgat
    41821 cgcctggaag atcataagat cgccgggaaa tccctgcgtg ggatcggcca gcaggttggc
    41881 ggcgagcggc cccgtccccg gctggtattc gagggcgccc atgtccgggc cggcaccggc
    41941 cacgcgcggg ttcccgtcga ggtccgccgc gccggccatc cacgcgaggt tggttcccgt
    42001 gtccacgcaa ggcgagccgg gccggaggcg atagtcgccg gaggccgggt ccatgaaatc
    42061 gggcgcgtcg gccgtgttgc cggtcccggc cggagccggc gcggcgcacg aaaaatccac
    42121 ggtggaggca agcgcgtaga ggttcgggtg ccggtcggcc tcgttgtcgt acgcgatgac
    42181 gttcaccgac gacgcgcccg acagatagag cccgcctccg tcccgggcct cattgccggc
    42241 cacggtgcag ttctgcgcga gcgctgcgcc ttccaggtaa atgccgccgc cgttcgtggc
    42301 gacgtgatcc gtcacgagca ggttgcgcag ggtgctgccg ccggcgccgt acacgccccc
    42361 gccgtccacc tggcccgccg cgacggtgta attgccgttg atccggcagt tttccgcgat
    42421 ggatcgatcc agccacaggc cgatgccctc cacggagttg ccggccagcg tttcagccca
    42481 gttgcccgac acggtgcaat gcgcgatccg gctggtccac acgagggccg ccccggcgcc
    42541 gtattggctg tactcgcccc cttccacctt gttgctcgac acggtggaat gggtcaccac
    42601 gctgccgtag tcgaggtaca atccgccccc cgcggccgtg ccgttgggcg cactggccac
    42661 gttcccgagg atgcggcagt tcgtgatcga ggcttcctcg ccgtagatcc cgccgccctc
    42721 ggcgtcgtcg ccgtcggacc aggacaccgc cgtgttggcc aggatgtcgc aatcccggat
    42781 cggcgcgccc tcgacggcgc agatcccgcc gcccagcgca tcgtactgat cggcctgcgc
    42841 cgtgttgttg ctcacccggc agtccacgac ctgcgcgccg tagctgtaca ggccgccgcc
    42901 ccaggcctcc cggtagcttt gcccggattc ggccaagttg ccggacagga cgcaattgct
    42961 gatcacggcg ggcgcttcga gggatacgcc gccgccgaag gtttcatcgt atgaccgggc
    43021 ctcattgtcc acgaacgcgc agttctcgat gcggctgggc ccccacacgc acgcgccgcc
    43081 accgaacact tcctcgtacg ccgcgatcgc caccgtgttg cttacgaaca aactgtcggt
    43141 cacacgcgtg ttccacccgt aaaagccgcc cccggcccgc gtcgccgtgt tgctggtgaa
    43201 gatacagttg gtgatcgtcc cgccctgacc ggaatagacc ccgccgccgt tgacggcgcg
    43261 cccgccccgg atggtcaggg cccgcagcgt ggcgtcgcgg tggtccatga ggaagcatcg
    43321 ttccgcgccg ttgccctcca cgatcgccgc cgcgggatcg tccatccctc ggatggtaag
    43381 gccggaccgc accaccacct gccccgtaag gctgtggacg gcattggaaa gccagaccgt
    43441 cgtcccgccc acccccgcgt ccactgcctc ctgcacgttg gtcgccgcct gtacccagtt
    43501 cgtgaacggg aacacgtggc ccgcgccggg cgcgacgtac gcctccgccg ggccgacctt
    43561 gatgtagtcg gccttgacca tcgtgtcggt ctcgtgggcc gcgttgctga cgatcagcga
    43621 caccgagtag aacccgcacg cgttgtaggt gttggtcacg atccgcgtgt cggcgccctg
    43681 ccggtccggg acgccgtcgt tattgaaatc ccagacgtaa tacagcccgg cggtgttggt
    43741 gccgctgacc catcccgtga atatgacctg cagcggcacg aatccctcgg tcgtgtccgc
    43801 catgggattg cacacgagag ttccttctcc cggttgaaac tcgtacgcgc ccatgtccac
    43861 aaggccgccg gtgatccgcg gccggccgtt gaggtccgcg gccccgacca tccagggctg
    43921 gttggtcccg gcatccaccc cggcggaccc gggccgcagg cggaaatcga acgcggccgg
    43981 attcacgaag tccggcaggc gttcggtgtt gccctcgccc ggcggcagcg gcgccgccgc
    44041 ggtgtattga atcgcggcct ccggcgcatc gtagttaggc cagtccaccg cctcgttgaa
    44101 aactatgatc gaattgaaga ccgtgccggt cgaatacacc accaggccgc cgccctgcgg
    44161 cgcccaattg ccgaccaccg tgctgctctc catgatcccg agggattcaa gactgatccc
    44221 gccagctgtg ttggaggccg tgttgcagta gatcatgcaa ttgcggatcg tggccgggac
    44281 ggaataggcc cacagcgcaa tcccgccgcc atttctggcg gtgttggcgg ccacgatgca
    44341 gttctccatc gttccgccgt cgctgcagat tgcgccgccg ccgctcgcgt agccggcgga
    44401 gttgctataa atcgtgcaat cccggaccag cccgcccgag caccacacgc cgccggcccc
    44461 gttgccggaa ccggagtaga tgttgctcag gatgacgcag ttcgagatca cgcccccggg
    44521 ccggcagtac gcgcccgcgc cgccgttctg cccgacgttg ttgacgatca cgcagttttg
    44581 aacgctgccg gccgccacgc tgaccccgcc gccgcgtgcc gtggtccgac cgcgcgtgat
    44641 cgcgaacccc tccaccacgg cggcggcgtt gctgacatca aagcagcgca cggagttgtt
    44701 tccgttcacc gtggtcacgt agaaaccgtt cgcgctgcgg atcgcgacgc cgttcgtgac
    44761 attgatttgt gccgatagaa agtaggtgcc gttggtgacc agcaccgtgt cgccgtccgc
    44821 ggccacgtcg atggcctgct ggatattcgt cgccgcggtc acccaattcg tgtatggcgc
    44881 cgccgcgccg ggattggatg gcatgacata atgcgtggtc tgcgccatcg ctgcaccggc
    44941 ccacaacagg tttccaatga ttggaaaaat cccaagcccg gatttccaat gtctggaaaa
    45001 gttcatggtg atgcctcccc tttactccaa ctcgatccgg taatgccacg ggccgtttcc
    45061 cacggccgtc gcatccgtca cggtgttcat tggcgccgcg ccggcgatgc ccgactggac
    45121 cggcgtccat gcgccggccg gcagattggt ggagcgcgag agcgcgtagg ttttgccgtt
    45181 cacgctggac caggacacgg cgggggcgga agcctgaaac cggagacggg aaacctcgag
    45241 gagggaaagg gggtcgagcg gatcggtccc ggcgagaatt tcgtcgccgt cgcgcagcca
    45301 gtcggcgtcg gagtcggctt cgggatggac gaactcgtgg gcgcccatgt cccacgcgtt
    45361 ggtcccgtcg ttgttcccgt cgagcgggcg cgggatgccg ttgatgtcgc ccgccagctg
    45421 atttgtcggc gacccggcat cgacgcaggg cgaatttgtg gtcaggcgga aatcgccggc
    45481 ggcggcatcc acgaacaccg gatcattggt gatgttgccc gtccccgctc ccgggtccgg
    45541 cgtcgtacag gaagtctcga acaccggacc ttcggagtag ttgttgtccg agacggccgt
    45601 gttgaaatag aggatcgagt tcacgatcag cccgctgccc tcaattccac ccgctctgtc
    45661 cccggcgttg ttcaggacaa ccgtgcaatg gcacatctgg ccttctccga tacgcgctgc
    45721 cccggcccgc gagctcgccg tgttgtacgc gatcacacag ttccgcacga tgccggtcac
    45781 ggcccccatg cacacccccc cgacccattg cgccgagttg ctgatgatct cgcagtgctc
    45841 caggacaccc cccgccgacc catcgtagaa gaagacgcca ccgccatcgc gcgtggcgct
    45901 gttgcccgtg atgcggcagc gccggacaca cccgccgttg ttgaagaaca cgccgccgcc
    45961 gcaattgccg ggaaagtcaa aatagtcgcc cggcgcgtga ccgccgcgaa cggtgaaccc
    46021 atccaccacc ccagccggat ggttcatgta gaggcaccga ttcgttcgca ccggatactg
    46081 cgcgtccagc accgtggcct cgaatccgtt cacacttcgg gccgtgacgc ctttcgtgat
    46141 ctcgagcggg cttccgaggt aataggtgcc gttcgacacc cagaccgtgt cgcccgccgt
    46201 cgccgcgctc acggcgtctt ggacgttcgt cgcggccgtc acccagttgg tgtacggcgc
    46261 cgccgcgccg ggattgaccg gcacgacgta gcgcgtcgtc tgcgcggacg cccctaccgc
    46321 gaacacaagg cttccaagga ttggaagaaa ccaacacccc attttccaag gattggaaaa
    46381 actcatctcg cacctcctca ttccaactca atccgataat tccacggccc ggagcccacg
    46441 gccgtcgtgt cagtctccgt gttcgccggc gcgatgccga ggatgttggt cttcacatcg
    46501 tagtcgaacc cggccgccag attcgttgcg cgcgacagcg tgtagcgctt gccgtccacg
    46561 ctctgccagc ggaccgccac ccccgccgcc cccgcctgcc gcacgatctg cgtcaggccc
    46621 aggaacgaag ccgcgtccag cggcagggtc ccggccagca cctccgcgcc gtcgtccatc
    46681 caatcgccgt cggtgtcggc ctcgaccggg cttgtgccga tggccagctc gttggtgtcg
    46741 agcagccagt cggcatccga gtcggcctcg ggatgaaccc gctcatacgc gcccatatcg
    46801 tagcccgcga cgccgtcttg gttgccgtcg agcgggcggg gcatgccgtc gatatcggac
    46861 agaggcaggt tcgtcggggc gcccgcgtcc acgcacggcg agttggtggg cagacggaag
    46921 tcgtaagcat ccggatcgac gaacaacggg ttgccggcga tgttgcccgc gccggccggc
    46981 aggggcgcgg cgcaggagta cgtccacgcg ccgccggagt aattcgagta ggtggccggg
    47041 ccgatgtttc cataaacgat cgtattgacg atgtttgccg ccgtataggc gtaatagccg
    47101 ccgccccata gggtgccata cgttcgatta caggcgatgg tgcaattacg tatctggccg
    47161 ccccggctgt agacgccgcc cccgttgccc ccgcaggagt tgctgtagat caggcaattt
    47221 tccagcactt gagcgccgat attcaccccg ccgccgctgg tcagcgcgta gttggcctga
    47281 atccgggtat cgcgcaccac cccgccgccc tggttcatgt acaccccgcc gccctggtcc
    47341 cccgtgttgc acatcaggac gcaccgggtc acgaggccgg tcgagttgac gtacacaccg
    47401 cccccgcggt tggtcgcgta gcaggaggag gcgatgcagt tcgagagcgt cccgccgtag
    47461 aaatagaccc cgccgccctg gtaggcccgg ccgcgcgtca gggtcaagcc cgcggcgagg
    47521 gcctccggat ggttcaacgt caggcagcgg ccgctgttct gggcatccac aacggtcgcc
    47581 gccggcccgt tgaccgagcg cagggtcgcg gcgacggcga gttccagcgg cgaccggatg
    47641 gcgtacaccc cgtcggtcac gagaatctgc gagccgtgcc acgccgcacc gacggcggcg
    47701 gccaggtccg tcgcggcgac ggcccagttg gtgtacgggt actggtgcgt gccgccgggc
    47761 gcggcgtagg cgatcagcgg cgcgacggtg atgaggttcg tgcgggcgcg cgcgctggtc
    47821 tcgccggccg cattgctgac cgcgagcgcc acggtgtaca ggccggcggc ctcgtaaagc
    47881 tgggtcacca cgccgagccc atagccctga tcatcccaaa tcgagtcccc attgtaatcc
    47941 cattggtaat acagaccgct tgtgttcgtg ccggcggcgt agccggtcaa caccacccgc
    48001 aatggttcca ggccgatgag cggatcggcg gagggattgc attccagagc gccatacgcg
    48061 tattcgaagg cgcccatgtc ggtgatgccg ttcaggatgc gcggggagcc cgcaaggtcc
    48121 gtcgcgttgg tcatccaggc caggttggcg cccgtatcca tgcaggggga gccgggcagc
    48181 aggcggtagt catcatgggc ggtatcggcg aattccgggt cgcggtccgt gttgccggcg
    48241 ccggccggca agggcgcggc gcaggtgtat tgataggcgc cggcgaggta gtcgggcgag
    48301 gccggcgcgg agttgaaccg cacgatgctg ttttgcaccg cgctgttgcc cgagacatgc
    48361 gccaggcctc cgccggatgt gcccgccgag ttcttgcaca cggtcgaact ctccaacgtg
    48421 gaaaaactgc cggaaaaata cagcccgccg ccttgcgagc cggcctggtt gcggatgacc
    48481 aggcaattac ggacggtccc gcccagggcc cagacgccgc cgccgctgtt ggcccggttg
    48541 gaatagatct ggcaggcctc caacgtcccc gctccgtcaa tccgcacgcc cccgttcgta
    48601 tggctatgaa tccgcgagcg tcgcagcacg ccgccctcga ggcaggcgcc gccgccccag
    48661 ctttcgccgg agcgcgtgga gttgctccag atctcgcagt cggtggcctc gccgccgttc
    48721 tcgaagtaca agcccccgcc ggtattgtcg ctgcggttgc ccgtcacgac gcagttggag
    48781 accaacccat agcgctcgaa gaacacgccc cctccgtagt ccgcccgccc gccgcggatg
    48841 gtcagggcgc tgacccgggc ggcgggatgg cagaggtaga aacaacgcga ggt
//
LOCUS       DAOOTK010000012        48819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_562_length_48819_cov_6.225433, whole genome shotgun sequence.
ACCESSION   DAOOTK010000012 DAOOTK010000000
VERSION     DAOOTK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 48819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48819
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_48819_cov_6.225433"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK634_02765"
     CDS             complement(<1..807)
                     /locus_tag="PK634_02765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOW96690.1"
                     /translation="MDSRIDEFLRDWYVTGFRIRVTVSHEETISILADPVEKIACRKC
                     GAHLDVSRMPSFSSVVCPQCGLKQMVPALLGQFLLLELLGAGGMGAVYKALDQALGRY
                     VAIKVMKQAMGNDPQFVENFLREARAAAAINHRNVVQIYSCGQEKGQPYIVMELVSGG
                     RLDEQIGRGDPLDETRVLEIGVGVAEGLKAAHDIGLIHGDIKPANILFDKDHTPKVAD
                     FGLARFIAWQQNRGGEVWGTPYYIAPEKARGQKEDHRSDIYSLGATLYHAL"
     gene            991..1743
                     /gene="lgt"
                     /locus_tag="PK634_02770"
     CDS             991..1743
                     /gene="lgt"
                     /locus_tag="PK634_02770"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOW96691.1"
                     /translation="MDPVCFTLGGRPIYWYGVFMALAFLAAVTHWNWLGRRQQRPPGF
                     GSELAFWIMLAGIVGSRVAYVFSEIDEFRGRWLDVVRVDQGGLIFYGGFLGAILAVWF
                     LAWRRGEPFWALSDFTISALPLGHALGRVGCFLNGCCYGRPTGAPWACASHDALRHPT
                     QLYEAAANLLIYGVLLRALRGGAVKDRPGRLTGLYLMLYPPVRFAVEFLRGDPRMRWA
                     GLSVAQWMSLAMFLLGAALWAGWPRRRSPASP"
     gene            1740..2690
                     /locus_tag="PK634_02775"
     CDS             1740..2690
                     /locus_tag="PK634_02775"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOW96692.1"
                     /translation="MSAANPSGESGRLAFGGPPARLDAWLAAARPDHSRARWQELIRE
                     GRVRVNGLPRKPGHPLRAGDAVEFDIPPPEPVGLKPEPIALDILFEDDHLLVLNKLPG
                     LVVHPAPGHDAGTLVNALLHHCGPGFNVGGDQRPGIVHRLDRDTSGALVVAKTEAALR
                     GLAAQFKNREVRKEYLALAWGRPVPAEGTIETLVGRHPRHRQKMSARPRSGRPAVTHY
                     ETVQALGPASLLRVRIETGRTHQIRVHLAHRGHPVVGDTQYGRARPGVLPVEPERQML
                     HAERLAFRHPVTGAELEFQAPMPPDMRELLRVLSGAAGRD"
     gene            2745..3500
                     /locus_tag="PK634_02780"
     CDS             2745..3500
                     /locus_tag="PK634_02780"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOW96693.1"
                     /translation="MTTKSKPSDPRAGWLALARELESHLLHLREEGATEVEADPVQVR
                     RLAEPPVFPPPAAEGAPETADSLAAIAREVDACARCPLHSTRTRTVPGQGSTAPEIMF
                     IGEGPGADEDQQGLAFVGRAGQLLTKIIEAMGFTRDQVFIGNIVKCRPPGNRVPMPEE
                     MQTCLPFLRRQIALLKPKVIVALGATAVKGLLDVQSGITRLRGQWMSYEGIDLMPTFH
                     PAYLLRNPSGKKDVWEDMKAVLARLGREPPPRK"
     gene            complement(3520..4215)
                     /locus_tag="PK634_02785"
     CDS             complement(3520..4215)
                     /locus_tag="PK634_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96694.1"
                     /translation="MTDETASGSPAPLLPGTHAQILLLCLHEELGKAFLAAVCPRAAG
                     LEGELEIEGHRLHLRVLAGDPRLTAGWDEAVRHAHACALLARFLDVVSLDRIRAIYRR
                     MPLEKNVPLAMLLFRQEGEMDFKISCPSCGQKLWVRDSDGGRRGRCPNCQKGFRLPSQ
                     AGFLRGQLALPDSVATLTVINGRSASCRGALAHLIGHLGGAIVGVTSLDHETLKKSTV
                     RVELPEGDQTAHD"
     gene            complement(4267..4500)
                     /locus_tag="PK634_02790"
     CDS             complement(4267..4500)
                     /locus_tag="PK634_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96695.1"
                     /translation="MPRQFENLTAAELYCPRCRAARPVRERLLLVLPQADLHEYRCAA
                     CGESCGTREVKHGLAGAARAAPGGARLRPRAFP"
     gene            4631..5020
                     /gene="gcvH"
                     /locus_tag="PK634_02795"
     CDS             4631..5020
                     /gene="gcvH"
                     /locus_tag="PK634_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOW96696.1"
                     /translation="MNVPSDLRYTRTHEWARLDGGVLTVGITDFAQHQLSDLTYVELP
                     KVGDEVGAGDEVAVVESVKAASDIYAPCGGVITEVNAALPDRPDLINRDPYGEGWIFK
                     VRPASADEFDALIEPDEYEELLPGSEG"
     gene            5020..5688
                     /gene="lipB"
                     /locus_tag="PK634_02800"
     CDS             5020..5688
                     /gene="lipB"
                     /locus_tag="PK634_02800"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOW96697.1"
                     /translation="MSARAFFLELPLPFSEGVELQERLVEARARGDIPDTVLFLEHAP
                     VVTLGARGRTEHLKLSRDELAARGIELAPASRGGDVTYHGPGQLVMYPILKLGEREAD
                     AHGYLFNLEEVGLRTAADFGVQAWRRPGLNGIWTEAGKLAAIGFRIRRWVTMHGMSFN
                     VDPDLGGFQAIFPCGLREPVTSLRALLGGRCPALSAVRDRMAVHFGAVFQRPLAVRGG
                     NPMP"
     gene            5813..6136
                     /gene="rplU"
                     /locus_tag="PK634_02805"
     CDS             5813..6136
                     /gene="rplU"
                     /locus_tag="PK634_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOW96698.1"
                     /translation="MDAYAVVETGGKQYLVRPGEVVRVERIAAKAGDKVALAPVLAVC
                     SGQAATVIGAPVVEGATVTATVARELREDKVVSFKKKRRKGYRKKIGHRQTLTELKIE
                     AINAP"
     gene            6163..6417
                     /gene="rpmA"
                     /locus_tag="PK634_02810"
     CDS             6163..6417
                     /gene="rpmA"
                     /locus_tag="PK634_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOW96699.1"
                     /translation="MAHKKAGGSSRNGRDSQGQRRGVKVFGGQTVTAGSILVRQLGTR
                     LVAGENVGMARDFSLFALKPGVVQYDRNGKRVNVRLAAAT"
     gene            6476..7486
                     /gene="obgE"
                     /locus_tag="PK634_02815"
     CDS             6476..7486
                     /gene="obgE"
                     /locus_tag="PK634_02815"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOW96700.1"
                     /translation="MKAITFIDRARLYVYAGNGGNGCASFRREKYIPHGGPDGGDGGR
                     GGSVILRADKNVDSLLDLYYEPHRRAEHAGPGRGKQCTGRDGRDLRVKVPPGTLVRHE
                     ADGALVGELLADGQELVVARGGRGGLGNMHFATSSHRAPRECTPGTPGEQFILRLELK
                     SIADIGLVGYPNAGKSTLLRALTAARPKVAAYPFTTLHPVIGTLELGDAESLRIADIP
                     GLIDGAHRGVGLGHDFLRHIERTKLLVFVVDMAGVDGRDPSDDWDSLQRELELYNAEL
                     ARRPGLVVANKMDLPEAEERLKDFRKKTKTRPIPISALAGEGVARLREELVRRWETLA
                     GA"
     gene            7901..8278
                     /locus_tag="PK634_02820"
     CDS             7901..8278
                     /locus_tag="PK634_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96701.1"
                     /translation="MSYGGLARSCTISNNVSDETGGGANLYYGGAAVSALDVFKKKPY
                     EPENSNTGAQHLEHPVRRLMILLHPNTENAKDEGRDHTHHEAEDLIWGRRVLGFCWMP
                     GLHESFGDKVTGERVECLIAQED"
     gene            8403..13064
                     /locus_tag="PK634_02825"
     CDS             8403..13064
                     /locus_tag="PK634_02825"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOW96702.1"
                     /translation="MTRRVGFLVFGLLPALLLPAAKADITSDLQEIADGLVDQGSPAA
                     LLHVEDSALGTNALASGVRYPNEIIEGESVTGELAAAHTMWFHCATVSGQYYGVVVTG
                     FTADVSLQLVNESLGFVTNRSGGWTGWTSQDDGGYDVGLSSTGNTPFVLRFYSFTNTI
                     ARATQWITEQMEMQDVVGLSVALVDGQEIAWARGFGYADLENGIPVTTDTVFRVGSVA
                     KLFTATAALQYHDQDELGLDDPVTNHLPGLRLLYRYPETDSITVRDLLDHHSGLPGDL
                     INSGFTTEPVVGRYAWLTNCLVTTYPIYPPRLIFSYCNSSFTLMEGVIEAVDGSGAPF
                     TTLVASNLFEPLGMEATSFLKDKPAIVSQLAKPYIDGQAMPEEYANIYGTGGMYSRPV
                     DLAQYIRMILAGGVAPGGTRVLGTDSVAEMLTPQATNAPFAPFAPLTAGLGWEAVEWP
                     PLKYAGNLAFKSGWTMVYHANVAALPERDLGFAITLSSPAYIEKTATDFVLKHAVSDR
                     YGIPVPTNTVYFPGATQAVSQAELDALAGVYATGDGYHRVESHPGSLTLVLNAESDEA
                     KRFTNMLLRADGWFSPGASPTNGMLITNIAGRTMLVWRIAYGGSVLTNLHCGERVNPA
                     PLSAAWSNRLGKAWFVLDESPAGYSIALGTSTRLTFTNRDGVLLVQCDYVEGSQVLSP
                     TNDNTAFVSGLINRGDSAVQVFTSNTFEYLRYCGYTFQPAESFPVLYPGSMTNLSLAA
                     GETRWFAVPGWTGDPQGLTITNASAPLRLNLYTPEGYLLGRGTSALPWICPTGGTYYL
                     SVGAMNNASGNLRAYSLTNTIAKATEQIEGLMVSNGVVGLSVALVDGQEIAWARGFGY
                     ADRENGIPVTTDTVFRIGSVSKVFTTTAALQYRDRGKLGLDDSITNYLPGLRLLDRGG
                     FPGTDAVTVRDLLDHHSGLPGDLLNSSMTTEPFGGRFAWLTNYLAGTYPVYPPRLVNS
                     YCNSGFVLMEGVVEAADGSGTPFTDLMSSNLFEPLGMDASSILKDKPDITNHLARPYV
                     NEQVMPEEFINIHGTGGMYSRPVDLAQYIRMILAGGVAPDGTRILETNSVAEMLTPQA
                     TNAPLEPFVWETVGLGWDTVRSPELSYAGDLAWKNGITTVYRANMAVLPNRDLGFAIT
                     VSSPAAFAGGPLYFVLQQAVSDKYGIPVPTNTVYFPAATQAVSQAELDALAGIYAAGD
                     GYHRVESQPGSLTLVLNAHSDEPPPDGSRVVVTNLCLRTDGWFSVGSPPSFSIVITNI
                     AERTALAYRQASGALSETSLIGERFNPEPLPAAWSNRLGKAWYVLDESPVSYFVVMGF
                     SPRLAFTNRDALLLVQSEYTGGSHVLSPTNDNVAFVCGLVNAEDSAVQVFTSNTFEYL
                     RLAGYTWQPEDSIPELTPGVPVSGTNLSTQCNWYRADLPVGTFTLTCSDTNIRMIVYG
                     GMAAPVIAIGSYTFAQTASGSVYVALGTMSSSVPYELDAGRATLILERIEGGPSGMAF
                     TWISQPGTNYTVEVATNLCGDPAFAPLASNLPSSDEIVTTWTAAPPASALEFYRVRLP
                     P"
     gene            13168..14913
                     /locus_tag="PK634_02830"
     CDS             13168..14913
                     /locus_tag="PK634_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96703.1"
                     /translation="MLAILALGGAAQAQWVTQNISLQPGWNAVYLEVQPAPSDCETIF
                     SGHPVEDVWLWNKRFSPVEFELDPDTPLAPNPHWLIWLPSTNTESFLSQVFGLQACQS
                     YLIRVPTSAAPFEISVKGAPVIPHLEWFPHALNLVGFPINPVAPPTFSEFFTAAPQVD
                     TSRGFDNELYTLDASGRGRTIVQPYRDRIQPGRAYWVKCSGALDYEGPLRVTTTIAGG
                     LDFGLVLEEIGLSVANVSTTTAVTVTLKQAASEAPPAGQAELAGPVPLSYMISTLGTS
                     NDLAWTNLPAKGLTKTLAAGETWSLNFGCRRNDMAPYTPAGTNGAAYQSILEIADGSP
                     SLLVRVPVIAEKDNVRRLGLRQDEYDLDDHHSDEGLWVGYAVINEVTCPSYSSTNLLP
                     TESDCRLRLILHSDAYGRVKLLQRVYLAWVGPATNGEYGLFMDEASVPTDSEDVDLIS
                     SAAFPLMAPVALANCDTNRPPTGLTNQLCGTVVLAFNDPTNPFLHRYHPLLDNKDWNF
                     HSYSNAVESRDVTREILMDFNDNVFTGGAPDPFYGISEIGGTYEEILTGLREQPIHVK
                     GAFYLQRVSLVNELH"
     gene            14975..16084
                     /locus_tag="PK634_02835"
     CDS             14975..16084
                     /locus_tag="PK634_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96704.1"
                     /translation="MRTKKRNPFLLHPSSLILLLCSSFILDPSSFSFAQSGAPAVINY
                     QGRLYDPSLGGTGGPLTGVQQIEFRIYDNITGGTLIWGRQFPVSCTDQGVFNVLLNDG
                     GTWLAGATNHLPSVFLGSDRFLELTVVGHGSAISPRQQLVSAPYALQAAHAADALAAG
                     SGFTVNGGVLAVNGLLNMSGLATLNSGLVVRGSTALAGTLGVGGATVVSNTLHVTGNF
                     DVAAPSTIAGFGSIPIGGIIMWSGAPAGIPSGWALCDGGTHEGVPTPDLRNRFVVGAG
                     GAYAVSSTGGVDTVTLDQQQIPSHTHPVYAPNNDVNGSTSQGWPANNNHQSFRSSDRG
                     REYTINSAAVGSIGGGQAHENRPPYYALCYIMRVK"
     gene            16149..23609
                     /locus_tag="PK634_02840"
     CDS             16149..23609
                     /locus_tag="PK634_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOW96705.1"
                     /translation="MVGKKRAAFSNVWKTGCAWLSVLALPAAGAQTPQLTAEFLNPWS
                     VPAPGGLYSNYYVNGVGAPAFGGSTLTNILTAPDGSSSTVTYRSPSTGLVLAFSQYGQ
                     PVESDVAQYDLGDVIPWPAGMVSTSAPPVGFEAVRVGNNTNAYYQITNPVGAVIYLPS
                     TRQVVAAAGGNVEIGWLTTGGVVRTVYTISAVPAERPARVFWTEPPYNAPAVNLSANG
                     QQVFACIHYNNYVTRPVMEAVTTQNAQGEWVVTTQLVSGVWLDGEGVAQKIRAMDVEG
                     IALLEYYADGTFTRSIDLQPIQVMPPTVNIIEADIGSRLLPTDHYYGVDDLIPRIMAG
                     AGDDGRVLKVGLDGPKENWVFAIRRTVEDPWDVEIFWEHQDDRGVVWPFEVDWYALDW
                     PDHATRYVLGEALTNTAPIFLPDSLAAALPNEEPAGIASVAVDGRSFSATAPGHILVQ
                     YSTHDNMWFDVLRAVRHDDRTEYDPTPQLWDIGREILPFADDNYVLYFDGTNEYVEAT
                     MDAGFHGQDGFTVEAWAYRSGRIMNKDGPLVSHTASEGFAGSNDWEIAYGWGSDVYFW
                     THAGTASWSMSGTADLNQWHHFAGVYDGTNVLLYVDGVLRASTAASTALGVANRPLMI
                     GHSVRQNAYFQGKIDEVRIWNTARRAAQISDHMYRSIQGPEDDLAACYPLDEGAGTHI
                     RDAVDGSYGQFHGTPQWSASFHLANTNVSELAEFPGYIYSGRAYNVNRYHYPDEDDPG
                     RASYIFAVNTNDFEIWWARPSSQTDLPSQVYYPSLAVHYHATWPTNAPQIVIAGGRGS
                     GDDVLSAPSIYYQNDGALPGYNPNEEHALVLGGKAYALRDDLNRTNSSQPFVLVDGLD
                     PQTARPTMRTYGVVRTNADYRFEYSVKAGSAIIPPMPLSAMPPCSNTTYAWGPAWEDR
                     KESWWAKAAGNDGGDTNAGMRFFYQVQPSFWFPALGAGSQPALGREVPWLPTNSPPLQ
                     GGSYGTPLAMRYDISWPADRPEMQIAQTLTLATRGLPDIWNQLSVDVIYQQSRTNGHG
                     DSVSLFDPMVEHAVDLDISVINAMVSAGLARKDLTSSRYILSGLAPSLRSRMYYDPDR
                     GESGQLVLGGEYRTTLTGGGYLLPNLLDTQDREDAMTMADPLAGDKKTAWVSAVNALP
                     TNITLIVPNVPYVKAALCSALSAQSNAVGYVTLVFNNSTNAQQVPPSLPVSLSIIKVV
                     PELYNAFLDVIESDDALDEKLSLRYAADYAGRAEEYDFDWRWAEPEGGLPPTTNYAGW
                     SVYGAGIVQGELGVNIEGSSPFTLADHYFAVRYKRHDGLGPTGTNWSEWSYNLAPGWI
                     QRVMNAINPYEQRLHDMIGNSPNLMLSMIGQAGPPYEGAVALNDDAVNSAGLIQIYQT
                     VLDRAISMSLDAGMDDTANNDALLFAASRLNDLYMLLGNEAFADAQDPTIGFGTDSTW
                     FEHYGAAWSSLFCFMNQFPTLLDEELGLLRGRDDTLEPSTELTPVFNRLIWNYTRGIS
                     GGEVAYAMSYNIKGDPTSTVGVVTAEDAKALYPQGHGDAWGHYACALAGFYRLLSNTN
                     FGWNTTPGATLVGNAAVSSDFFDEQKFAEAAAAKARTGAEIVSRTYRKHYTHATGGRR
                     SFCGDSQTNRAWGVDGWASRAGQGAYLDWVAGNSLLLDNLTNLVQVGGGDQPPEGIQR
                     IDRTTVPELDDIAANLEQIQKQLDHADLGLSPLGLDRDVVPFDISAAEIDAGQTHFEQ
                     IYARALGALDNAAAAFDYAQGCTEKLREQWDSVSDFTGAAAESEVDYHDRLLEIFGYP
                     YPDDIGPGKTYPQGYAGPDLVNYQILDLEDLLGVAPTGGAQEVVTMYRVTFTADNNRH
                     DYTYTTNFFTNTTLYVNDKGLKVKPPSWTGRRPAQGDIQSALSDFVKAYYGFLKARDE
                     YANKMAGLSFAYEHFRDTASRLSNEWLVATQVADKKKTLAGWMAALEILAELSEIGSS
                     YGENMAKAVEKALPDIEMQAFPPSVNVSVSIAKAAAEAGNSTVKFWWEVATHGIKSFA
                     ALGKVLGENAVIDLELGTATNALSSEILWGSREVEEKLREQTVVADELMAQIEEMCAA
                     EQQYLAVLKKGERLISERARFRARTAQRLQADRYADMAFRIFRDEALAKYSATFDLAA
                     RYCYLAAKAYDYETGLLGTDTERSPGSLFLSDIVKSRSLGRMQDGTPLAAGTEGDPGL
                     ADAMARMKADWDVVKGRLGFNTPDTETSRFSLRTELFRTSPSTSSDETWRNTLELKRV
                     DNLYDLPEFQEYCIPFSSTTNREPAIVIPFSSYVLSGHNFFGKNLAGGDNAYDPTHFT
                     TKIRSVGIWFTGYNVSFNTNLVTGGGLANEPRVYLVPAGQDIMRSPTRGSETWRTWRV
                     FDQALPLPFDIGRAELDDPDWIPLFDSLGGTLAQRRRHASLRAYHDRGQFDASETCSN
                     ARLVGRSVWNTRWLLIIPGRTLLSDANEAIERFIYGAKLADGTRDGNGIKDIKIFFQT
                     YSIAGE"
     gene            23609..24493
                     /locus_tag="PK634_02845"
     CDS             23609..24493
                     /locus_tag="PK634_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96706.1"
                     /translation="MTAKRHVERNGAWKPSRFLLLCILVSSIQHPVSTFAGMPQPMVV
                     YYGQAKDEYGLPYRNGADVILRIGTNEITRHTINGSISPGVNFTLYVPIDDGRDDTRY
                     VSYAATTGQCVTIVVVDDRGERTIMEGAAVPCIEHPGDLLRINVTAGMDADMDGLPDE
                     WEWQLLLWGDNPLYHTVEDIVGSDDYDGDGQPNDDEYRAGTFAFLDYDYFFAERLART
                     ANSRLRVEFLSVAGKVYRIESAPFELAGWSFAWLPCEYALTDTGALQTGPAEGTGNWF
                     SFYVPDVPSNHVLRLKVE"
     gene            24697..25350
                     /locus_tag="PK634_02850"
     CDS             24697..25350
                     /locus_tag="PK634_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96707.1"
                     /translation="MNLQVGDLGSMYVEQFAPLDGDGSWGLSDTYALGRGLSNSLAVG
                     QWTLLARHGSDVNSFCGFNLRTSTSTESFKTDEILRFGINYDEGANAMRVCFSTNADA
                     DYAYLDLGDDDVRDADLEYSIAWSTVAGSFTLGVGNGKDYVEVTADLPSGSPVAMLGA
                     GIFEMNLDDKDDLTFDDYGISVIPEPGAAGTVVLGAWLLQRLFRSRIRRTSRHVLQV"
     gene            25483..26007
                     /locus_tag="PK634_02855"
                     /pseudo
     CDS             25483..26007
                     /locus_tag="PK634_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611846.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(26293..26369)
                     /locus_tag="PK634_02860"
     tRNA            complement(26293..26369)
                     /locus_tag="PK634_02860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26333..26335),aa:Val,seq:cac)
     gene            complement(26439..27092)
                     /locus_tag="PK634_02865"
     CDS             complement(26439..27092)
                     /locus_tag="PK634_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96708.1"
                     /translation="MNGQGEETGPAHRGSEHELSLVARSAQCILWHAEVEDHGGRELV
                     WKMRVFAEERGEFVDISEIGENFRIHAWYFGRGGEQALRMGEFATGQIRSGAGEYRQE
                     FVCAARDGSTLWFQEDTRIVKLGEKKWHLYGVCMDITGRKRAEQERERILGELKAALA
                     DVRQLRGMLPICANCKKIRDDAGYWEQIESYMRDRIHVEFSHSICPECEKKLYPDLR"
     gene            27192..28229
                     /locus_tag="PK634_02870"
     CDS             27192..28229
                     /locus_tag="PK634_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96709.1"
                     /translation="MKNQTAEGLRLRLEGVGLTASAARRIQAAVLRRGVFPGPAPGLP
                     AALLASVRERSEIPRLALLEKTVSPRDGFAKYLFRGAGPEPFEAVRIPLLHRPDDRKY
                     VACVSSQVGCALGCAFCATGRAGFVRDLAPWEIVDQVLHVRDDSPHPVRGVVFMGMGE
                     PLLNYDAVIAAARILSEPCGPAIAAKAITIGTAGIVSAIRRFTAEGHPFRLLVSLTSA
                     DPALRGALMPVEQAHPTRDLVDALREYHAATGRRVTLAWTMISGFNTGEADAQQLADL
                     ARGLPVKLDLIDVNDPSGRFRPPDAEELGAFRDALRRHLGAPVARRYSGGADIRAACG
                     MLAGCGTAKMP"
     gene            28268..28492
                     /locus_tag="PK634_02875"
     CDS             28268..28492
                     /locus_tag="PK634_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96710.1"
                     /translation="MKSRLPIVVLLPVLFCLPALAEIQSRAVEYRAGDAVMEGYLAWD
                     DPVRGAAYNEKADRRSWRAMRNFLEETLGR"
     gene            complement(28511..29725)
                     /locus_tag="PK634_02880"
     CDS             complement(28511..29725)
                     /locus_tag="PK634_02880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOW96711.1"
                     /translation="MKYRKALLQRVGWAAVAPAWLGLILLAVLGGGLARRMGIGETAP
                     AFLLPLVLAAGVLVLGAAWTLICARLFRVFYGGEQQEVLQAGNWALNRAMGRRVPADE
                     TEGPGEPSPVQQNLDDWLKGMVEEMRQRQEAQSFEIQRDLDLARDFQLAYMDRPYPAI
                     PAVHVPGHLRLSFAHRYQPASALGGDFYNILTLASDCAGIFIADVMGHGTRSALITAI
                     IRTLIDDLAPQGRNARHFVKELNRQFCGLLTNIPGTQFASAFYFVADTTSRVATFASA
                     GHPAPIYAHRGLGRLQRLDVPMPRGAAIGVIPQEDYTAGYCRLNDGDLYVFFTDGLFE
                     AHNAAGEEFGIQRMEKALQKLMYQPARAVVDGLMDAALAFVGNEPVADDICLVAVEVS
                     DKAIDKSAPAAP"
     gene            29866..30306
                     /locus_tag="PK634_02885"
     CDS             29866..30306
                     /locus_tag="PK634_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96712.1"
                     /translation="MTETPHDPKLAAATDIIFNCPFCNKSLAIDLRGAGLMVTCPDCG
                     RDVQVPAPAPGDTLEEVPTPPMTVSAEALAAAHTKIERLVDSLEEIRERRRYLEHLRT
                     ENMARFQQIGGEVEVIQNSLDRIVGLLQAAAADEKKSGEPPPES"
     gene            complement(30330..31787)
                     /locus_tag="PK634_02890"
     CDS             complement(30330..31787)
                     /locus_tag="PK634_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW96713.1"
                     /translation="MSALRFTRSIRWRMLLWLAILLGLALAGLGVTAYQLQRLHRLRD
                     IDQQLERRMALLSLDLRRRGPPPPGPDSERPPPPDKGPPPGRERHRGPPGPWTFGLSD
                     QTKALFDETDPEGFYFATWSRDGAPLRCSSNAPPGIVRPAWTGDDPRIRHRTRTALRE
                     AYYFTERGDGVLAGLPIQSELKTLRRFAGWLILAGGVVLGLGIGGGWLLVGRALRPLR
                     RIGETAGRISSGNLAERIDLAGTESELGRLASVLNAAFARLEASFAQQKQFTADAAHE
                     LRTPVAVILSEAQATLARERTPGEYRAALETCQNTARQMRRLIQSLLSLARFDAGQVR
                     LERAPCDLAQLAQTGADFVRPLAEIKGIGITCDFTSAPAVGDADRLVQMLVNILTNAV
                     DYSRPGGEIRISTRPDSNAASLSVSDTGEGIPEEDQPHLFERFYRGDKARADTGSHAG
                     LGLAICKAIADAHGGRIEISSRPGAGTTVTMRIPS"
     gene            complement(31784..32437)
                     /locus_tag="PK634_02895"
     CDS             complement(31784..32437)
                     /locus_tag="PK634_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW96714.1"
                     /translation="MRVLVVEDEPRLLNNLARALREEGYAVDTAADGADGLGKAEEYD
                     YDAIVLDLLLPRLNGLDVLRRLRALKKTPVLILTAVDGTKDRVRGLDLGADDYLAKPF
                     DLPELLSRLRALIRRAAGTARPVLEAQDVRIDTRSRLVTRAGRDVPLTAREYSILEYL
                     VLHRGEVVTRSTLYMHLFDETSDTLSNLIDVHVFGIRKKLGPDIIATRRGHGYCIEP"
     gene            complement(32496..34343)
                     /locus_tag="PK634_02900"
     CDS             complement(32496..34343)
                     /locus_tag="PK634_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOW96715.1"
                     /translation="MNKATIFGVSAALLALAGPARAVVNADSAANYGGGWTNGSNGGG
                     GFAAWSISGDAGGGWAGNGIWDSANAGLSMGSAFGYVAKGAGSYINLDRSFSQAMNTG
                     DVFSIDLGLNYDAGAGGSKGFALRTAANREIVTVNQAGSAAITVNGANALTNYGTAAM
                     HWTFTQVNATQVTVYATGRSGSEAYSATINATGLCFLANIHFYASAITNDEYAELRQV
                     YFDNLVLSQGTAGTGAFYYAVEDSRATVTGLAENATGAITVPSTLGGYPVTAVGRAAL
                     KDRTNVTSLAFASGVSVTNLGASAFQGCSSLALAVLPSGIGSIPPALFHGCTGLVSVT
                     IPAGVASVGDSAFVGCRALASVTLPAGLTSLGESAFLNCRNLASLDIPDGITSIPGQL
                     CYECRGLGSVAIPAGVTNIGYAAFYNCLGLSSLSLPAGLKTVGHSAFHGCDGLDSVSI
                     AAALGSVGDEAFYACAGLQTIYFNGGVTGLGVGVFGQCAALDGVYFQCDVPALGADGG
                     ADMFTGASEPTAYYLANPSSWSAAFCGAPAEEWTPLLLGPAVTAEAFRFSVDWADGRR
                     VRVQASTNLLNAAWLDLATQTVAGGACEVSDTNWAAFPQRFYRAEPAED"
     gene            complement(34354..36351)
                     /locus_tag="PK634_02905"
     CDS             complement(34354..36351)
                     /locus_tag="PK634_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOW96716.1"
                     /translation="MHTRAKFILAALAGTALLPAGAWAADADYVENAGFTTTVTIAYA
                     GASATLVNHAGAGVTITQNGAMITATSTIAGVEYILSGAATGGYLKIQGAPASKVTLN
                     GVALTCTNGPAINVQSTNRCFVVLADGTDNALADSAVYTESGQGSLYSAGPMIVSGRG
                     RATVTGRKSHGIYSAGYFRMRGGDVAIPSAVKDGIHAVRYFRMDQGTLTIAATSDGID
                     ADAGTVEINGGAIAITSVADDTKGIKCDGTFTVNGGAMNVTVKGVQSKGFKSTGAMTL
                     NGGAMNFVLTGNMYLVITNNYGDPSYCTAIKGDTNITINAGTITISHVGTAGKGISAD
                     GSIVVQGGVLDILTSGGCSAVFTNDLGVKDVAAADGLKADRNLDILGGTITAVSQGNA
                     GDAISCEGAMTIGVAGTTNAPCITAATRGQKVAVSASDYSNPKAIQAGGNLTVNGGTI
                     RASTRNDGGEGLESKARLAVNGGNIEITAYDDCINAASNLAINGGRIYCYSLGNDGID
                     CNGPMQYTGGTIVSSGTTAPEEGFDCDMYTFAIGGGVMVGTGGGSSTPTAGSSTQRSV
                     LYSTTTATSGTVIQVKSASTNIIVYRLPRSHTGGGGGPGGGGMKLLFSTPAITNTTYT
                     IVTGVSVAGGTEFHGLYTGGTVSGGTTTRTFTASSMVTTVN"
     gene            complement(36472..37662)
                     /locus_tag="PK634_02910"
     CDS             complement(36472..37662)
                     /locus_tag="PK634_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOW96717.1"
                     /translation="MPTNDSKSPRDFIAGHIVDMPRSGIRDFFDIVSTMKDIISLGIG
                     EPDFDTPWHVREYTVFALERGATHYTSNMGLLELRRAISKYVEKAFRASYKPETEILI
                     TVGVSEALDLALRATLNPGDEVLYHEPCYVSYRSDVILAHGTPVTVETKRDNQFRLTR
                     AMLEAKATPRTKVLLLNFPNNPTGAILRKEDLVEIAAFARERNLLVITDEIYAELTYD
                     GEHTSIASLPGMKERTIFLHGFSKAWAMTGFRLGYACAPPELTEAMMKVHQYTMLCAP
                     TMSQKAAIEALTKPQTAVVEMTAEYRRRRNYLVKSFEEMGLPCTTPAGAFYAFPYIGH
                     LGIASKDFALKFLDREKVAVVPGTAFGACGEGFVRCAYATSMENIQEAMRRMARFVQS
                     LKKG"
     gene            complement(37631..38110)
                     /locus_tag="PK634_02915"
     CDS             complement(37631..38110)
                     /locus_tag="PK634_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOW96718.1"
                     /translation="MDELLTILKNNALEKPETIARMLNLSVDAVKARIADYEKQGIIR
                     GYQAILNVDQLDISHVTAVIEVKVTPEREGGFDRIASRISRYPEVQSLYLMSGAYDLL
                     LFVAGKDLKEVAFFVSEKLATLDGVVSTATHFMLKTYKHHGVLMETQDADERLQVSP"
     gene            complement(38190..39326)
                     /locus_tag="PK634_02920"
     CDS             complement(38190..39326)
                     /locus_tag="PK634_02920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96719.1"
                     /translation="MGAIVVAVMLVVSALLWAVIFARLRYLETRFPPLDSSTPLPAPP
                     DGWPPVSVVIPARNEERDIAATLRALGRQDYPAFEVIVADDQSADDTVRIAEAAGREL
                     AAFRLIRGADRPDGGWVGKNWALVQGAAAAQHDWLLFLDADVVLHPAALKQAMAAAQA
                     GRADALSVLPAIECRTIWEKTIMPLFATLSALIEPMDRANRPGSAGSRLSGAFILARR
                     VAYEAAGGHRAVAGCLIEDMALAQNFKRGGFALRLFWTHDFLSTRMYDTFRDLWAGLV
                     RLSYPMMEYRPGRLLLAWAAAFAGAWVPWLALAGGWPSAAAGLLLIAVMPVALRKILQ
                     ILRLPRACGLLLPPAALVYCLAATWSAARHYAGRGITWKQRQYH"
     gene            39497..40243
                     /locus_tag="PK634_02925"
     CDS             39497..40243
                     /locus_tag="PK634_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOW96720.1"
                     /translation="MSLDNIRVVLVSPIYGGNVGSVCRAMKNMGLKHLAIAAPRGPLD
                     DLESRKMALHADDILENRQEYPTLAEAVSDCGLVAGCTARPGLYRSHAKSPREWAPHL
                     VQAAKKSKVALVFGTETDGLSNEELACCTQVIRIPSSPEYPSLNLAQAVLICCYEIFL
                     ASGTFELPGEFSPEASGAMREQMFAMWREALLSVGFMKGDKADHMMMGLRRILSRGTL
                     TVKDIRILIGMARQVRWAGSQLSQPGDAEK"
     gene            complement(40277..44722)
                     /locus_tag="PK634_02930"
     CDS             complement(40277..44722)
                     /locus_tag="PK634_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HOW96721.1"
                     /translation="MLVLAASFWGVWKARHTHERGISATAPTLQSRIPARDDPARSVT
                     TLVPSNSAILAPTAQPAMPDEKGFQLSQWPDAVVNRDPDFGTPQLIRRRSGLLSPPAP
                     GAAPREILAAFIRANDRTMTLHPSDLEPPNARLVQDYTTRHNGIRRVRWQQQHEGLDI
                     FGAHLTLNLTKDNEIINVSSRALYFPSVLFHPAETVDEARALEIAREKLGGGAVRKTY
                     LARKVWYPLDMISCVVGWELAIECEGNSDAHDLVIRADTGKIAFDQLLTWAEVGRAGP
                     ARRGGLSQAALPAETISLSVFTNDSPMPTTPGPYEPTNFTPVEASRLMLLLTALDTNA
                     SPEGWIPDGSNELLGNNADVYADWNDDSLPDQARLTGFPYRVFDRTVDLTKHPTNYVN
                     FSQVQGFYWANFYHDRLHALGFDEPAGNFQQDNFGRGGLGGDRFKIEVQNGASLGFSY
                     ANKAYYTGFGDGTEGKLTVSIFSRTSPLRDGALDGHLVLHEMTHGLTSRIIGNGYGLT
                     IVQPRGMAEGWSDFFALNLLSEPSDPPEACYPFGDYAAMYSPPTSNLYFGIRRFPYST
                     DMTKAPQTFADTDPNQIGFPPGIPCNPYFGAPEADQVHNVGEVWCLALWECRANLIEE
                     YGFTGSGLMEQLVVDGLKLTPDNPRFDQARDAILQADLVDNGCSNSIALWRGFVKRGL
                     GYSTFVPEPQSTVGIQEAFDLPFALTTTVTEAVGDGDGYIEPGETANMAVSLTSHELD
                     LSGVTGLLSTSLINITLIVSNAAFADVTAGMTVTSAAPFSFEIATNFPGFTDAMFTLR
                     ITSDKGWFDKPVVVRIGNPYDYPPEILNVAVMNITETNAWVHWNTGIPSTGCVFYGET
                     TNYGMTALSGHPMGTNHTADLTNLVKGTEYHFQIQAFGTNGLTSLSTDMTFRTRSRIY
                     VYADSLATQELGTIEAPFKSLQAAAEAAKTTGDEILVAMGTYTGSNPEAVLNLDGADY
                     DLTITGSYAPDFSEQNTEWYETMIDGQYQRRGIRLDNGANLSISGVTITRGSGEWGGG
                     VHIRKSFASIIQSSIHSCASMNGANAFGGGFYASLGSSVEFFDTDVRSNVARVGGGGF
                     GASQGTHLQFEQSQLAGNSSILSGGVEIELGASITVAGSVIAVNQCETAGGGLRIAPF
                     SSGTIQRSTFSRNFVSAPVNPDRDGGGGILVGGESSVMTICDSVIVSNHSAYGADLNC
                     GAQSEVHASYCNIDDIYGSLTTSNHLINADTMFANAAAGDFHILHGSPCIDAGSPDYA
                     GVDTDMDGESRPYGARVDMGADEFTDTDGDNMSDYWEANYFGSIAVSDGTGNPDNDGL
                     TDFQEYMIQTDPLNPDTDGDEGDDGYEVGLGMNPLNPDQDNDGMADGWEAHRGLNPFN
                     DDAGDDPDGDNAVNLDEYIADTDPLNGDSFLGLLSLGDFWSGKRVDWKGGANAWQMLE
                     FTESIFATGTWETLAAFPPPRPITNAVVIFGLNPTTGVLRIRAQR"
     gene            45097..47037
                     /gene="dnaK"
                     /locus_tag="PK634_02935"
     CDS             45097..47037
                     /gene="dnaK"
                     /locus_tag="PK634_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOW96722.1"
                     /translation="MAKVLGIDLGTTNSCMAIMEGGQPVVVPNSEGGRTTPSVVAFTK
                     SGERLVGAAAKRQAVTNPRHTVFSIKRFMGRKYEEVAHEISLVPYEVVRAGNGDAHVK
                     IGDKTYSPPEISSMILQKMKLDAEAYLGEKITQAVITVPAYFNDSQRQATKDAGRIAG
                     LEVLRIINEPTAASLAYGLDKKKDEKIAVYDLGGGTFDISVLEIGEGVFEVKATNGDT
                     HLGGDDFDQRVINWMADSFKKEQGIDLSKDPMALQRLKEAAEKAKCELSSSQQTEINL
                     PFITADASGPKHLTMSLTRAKLEQLVDDLVGRTVKPVENCLRDAKIEAAKIDEAILVG
                     GMTRMPKVQDIAQQLFKKEPHKGVNPDEVVAVGAAIQGGVLKGEVKDVLLLDVTPLTL
                     GIETLGGIATTLIERNTTIPTRKSEIFSTAADGQSTVEIHVLQGERKMASDNKTVGRF
                     HLDGIPPAPRGVPQIEVTFDIDASGILHVGAKDLGTGKEQKITITASSGLSKQEIEQM
                     TRDASSHAEDDAKRRETAETRNRADATVYETEKFLKEHGAKVSADKKASVESAVAELK
                     ESLKSGDADRMKADLEKVNAGMQALSGELYSQARAQQPGAGPQGGAQQAGGPGGAGPQ
                     GGAQGDVIDADFEMVDENKKKK"
     gene            47107..47397
                     /gene="groES"
                     /locus_tag="PK634_02940"
     CDS             47107..47397
                     /gene="groES"
                     /locus_tag="PK634_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOW96723.1"
                     /translation="MKIQPLGDRILVEPVKEEEVKKGGIIIPDTAKEKPTKGKVIAIG
                     TGKVDDNGKTIPFHVKKGDLVLMPRYGGSEVKIDDKEYQILREDDLLGIIQD"
     gene            47410..>48819
                     /gene="groL"
                     /locus_tag="PK634_02945"
     CDS             47410..>48819
                     /gene="groL"
                     /locus_tag="PK634_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOW96724.1"
                     /translation="MAAKQLVYDAEARQYILRGVEKLSRAVKATLGPRGRNVILDKKF
                     GSPNVTKDGVTVAKEIELEQPFENMGAQMVREVASKTSDVAGDGTTTATVLAEAIYRE
                     GLKNVTAGASPMELKRGIDKATEVVVEQLQKMSKKVKDRQEISQVATISANGDTTIGT
                     IIADAMDKVGKDGTITVEEAKSIETTLDVVEGMQFDKGYLSPYFVTNAEAMEVALEDP
                     YILMYEKKISNLQDLLPVLQNIAKSGRPLLIVAEDVDGEALATLVVNKLRGTLQCCAV
                     KSPGFGDRRKAMMEDVAVLTGGRFISEDLGIKLENIQLSDMGRAKRVTVDKENTTIVE
                     GAGKTSDIQGRIAQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVGAMTESEMKEK
                     KHRVEDALSATRAAVEEGIVAGGGVALLRAAKAITDLKLEGEAAVGRDIVARAVEEPV
                     RMIAENAGVEGSLVVARLRE"
BASE COUNT         8445 a        16085 c        16181 g         8108 t
ORIGIN      
        1 gagggcgtgg tagagcgtgg cgcccaggct gtagatgtcc gagcggtggt cttccttctg
       61 cccgcgcgcc ttttcgggcg cgatgtaata gggcgtgccc cagacctcgc cgccgcggtt
      121 ctgctgccac gcgatgaacc gcgcgagccc gaagtcggcg accttgggcg tgtgatcctt
      181 gtcgaacagg atgttggcgg gcttgatatc gccgtggatc agcccgatgt cgtgggccgc
      241 cttgaggccc tcggccacgc cgacaccgat ctccagcacc cgggtttcgt ccagcggatc
      301 gccccgcccg atctgctcgt cgaggcggcc gccgctgacg agctccatca cgatgtacgg
      361 ctggcccttt tcctggccgc aggaatagat ctggaccacg ttgcggtggt taatggcggc
      421 ggcggcgcgg gcctcgcgca ggaagttctc gacgaactgc gggtcgttgc ccatggcctg
      481 cttcatcacc ttgatggcga cgtagcggcc gagggcctgg tcgagcgcct tgtagaccgc
      541 gcccatgccg ccggcgccca gcagttccag cagcaggaac tggccgagca gcgcgggaac
      601 catctgcttc agcccgcact gcgggcagac cacggaggaa aaggaaggca tgcggctgac
      661 gtccaggtgg gcgccgcatt tccggcaggc gatcttctcg accgggtccg ccaggatgct
      721 tatggtctct tcgtgggaca cggtgacccg tattctgaag ccagtaacat accagtcgcg
      781 caggaattca tcaatgcggg aatccaacgg atatgctgaa ggccggccgt gaaagccttc
      841 gtgcccgcgc ccgtcattcc tcccgttgcg gcggcgcgtt gtccagtgac atgttttata
      901 aaacatgtca cagccgggag gggcgccggc agcaggagcg agggggcggg ggagttgtga
      961 cggggccgcc ggttctgcta taaagggcgc atggatccgg tctgtttcac gctcggcggc
     1021 cgcccgatct actggtacgg ggtcttcatg gccctggcct tcctcgcggc cgtgacccac
     1081 tggaactggc tgggccgccg ccagcagcgc cccccgggct tcggctcgga actggcgttc
     1141 tggatcatgc tcgccggcat cgtcggctcg cgcgtggcgt acgtcttctc cgaaatcgac
     1201 gaattccgcg gccgctggct cgacgtcgtg cgcgtcgacc agggcgggct gatcttctac
     1261 ggcgggtttc tcggcgccat cctggcggtc tggttcctgg catggcggcg cggcgagccg
     1321 ttctgggcac tgtccgattt cacgatctcc gccctcccgc tcggccatgc cctcggccgc
     1381 gttggctgct tcctgaacgg ctgctgctac ggccggccga ccggcgcccc ctgggcctgc
     1441 gcgtcgcacg acgccctgcg ccacccgacc cagctctacg aagccgcggc caacctgctg
     1501 atctacgggg tcctgctccg cgccctgcgg ggcggggccg tgaaagaccg ccccggccgc
     1561 ctcaccggcc tctacctgat gctctatccc cccgtccgct tcgcggtgga attcctgcgc
     1621 ggcgaccccc ggatgcgctg ggccggcctg tccgtcgcgc agtggatgag cctggcaatg
     1681 ttcctgctcg gcgccgcgct ctgggcgggg tggccccggc gacggagccc cgcatcgccg
     1741 tgagcgcggc gaaccccagc ggggaatccg gacggctcgc gttcggcgga cccccggccc
     1801 ggctggacgc gtggctcgcc gccgcccggc ccgatcattc ccgcgcccgc tggcaggagc
     1861 tgatccgcga ggggcgcgtc cgggtgaacg gcctgccgcg caagcccggc cacccgctcc
     1921 gggctggcga cgccgtcgag ttcgacattc cgccgcccga acccgtgggc ctgaagccgg
     1981 aacccatcgc gctggacatc ctgttcgagg acgatcacct cctcgtgctg aacaagctgc
     2041 cggggctcgt ggtccatccc gcgcccggcc atgacgccgg cacgctcgtg aacgcgctgc
     2101 tgcaccactg cggtcccggc ttcaacgtcg ggggcgacca gcggcccggc atcgtgcacc
     2161 ggctcgaccg cgacaccagc ggcgcgctgg tcgtcgccaa gaccgaggcc gcgctgcgcg
     2221 gcctggccgc ccagttcaag aatcgcgagg tgcgcaagga gtacctggcc ctcgcctggg
     2281 gacgtcccgt gccggccgag ggcacgatcg agacgctggt cggccgccat ccgcgccacc
     2341 gccagaaaat gtccgcgcgc ccgcgatccg gccgccccgc ggtcacgcac tatgaaaccg
     2401 tgcaggcgct cggccccgct tcgctcctgc gcgtccggat cgagacgggc cgcacgcacc
     2461 agatccgcgt ccacctcgcc caccggggcc acccggtcgt gggcgacacc cagtacggcc
     2521 gtgcccggcc gggcgttctg cccgtcgagc cggaacgcca gatgctgcac gcggagcggc
     2581 tcgccttccg ccatcccgtc accggcgccg agctcgaatt ccaggcgccg atgccgccgg
     2641 acatgcgcga gctgctgcgc gtgttgagcg gagcggccgg ccgggattga agccctttca
     2701 ttttgcttcc cgccggcgcc gttcttgggc atcctgaacg cccgatgacc acgaaatcca
     2761 aaccgtccga ccctcgcgcg gggtggctgg ccctggcgcg ggagctggag tctcacctgc
     2821 tgcatctgcg cgaagagggt gcgaccgagg tcgaggccga cccggtccag gttcgccggc
     2881 ttgccgagcc gcccgtcttc ccgccgcccg ctgccgaagg ggcgcccgag acggccgaca
     2941 gcctcgccgc catcgcgcgg gaggtcgacg cctgcgcgcg ctgcccgctg cacagcacgc
     3001 ggacgcgcac cgtgccgggc cagggctcga ccgcgccgga aatcatgttc atcggagaag
     3061 gcccgggcgc ggacgaggac cagcagggcc tggcgttcgt cgggcgcgcg ggccagctgt
     3121 tgacgaagat catcgaggcc atgggcttca cccgggacca ggtcttcatc ggcaacatcg
     3181 tcaagtgccg gccgcccggg aaccgcgtgc cgatgccgga ggagatgcag acctgcctgc
     3241 ccttcctgcg gcggcagatc gcgctgctca agccgaaggt catcgtggcg ctcggggcca
     3301 ccgccgtgaa ggggctgctg gacgtgcaga gcggcatcac ccggctgcgc ggccagtgga
     3361 tgagctacga gggcatcgac ctgatgccga ccttccatcc cgcctacctg ctgcgcaatc
     3421 cgtccggcaa gaaggacgtg tgggaggaca tgaaagccgt gctcgcgcgg ctcggtcgcg
     3481 agccgccacc gcggaagtaa cccggcgccg ccgggcgggt cagtcgtgcg cggtctggtc
     3541 gccttccggc agttccacgc ggaccgtgct tttcttcagc gtctcgtgat cgaggctggt
     3601 cacgccgacg atggcgccgc ccaggtgtcc gatcaggtgc gccagcgcgc cgcggcagga
     3661 ggcggagcgg ccgttgatga ccgtcagcgt cgccacggaa tcgggcaggg ccaactggcc
     3721 gcgcaggaag cccgcctggg acgggagccg gaagcctttc tggcagttcg ggcagcggcc
     3781 gcgccgcccg ccgtcgctgt cgcggaccca cagtttctgg ccgcaggaag ggcagctgat
     3841 tttgaaatcc atttcgcctt cctgccggaa cagcagcatg gccaggggta cgtttttttc
     3901 cagcggcatg cggcggtaga tggcgcggat gcggtcgagc gacacgacat ccaggaaccg
     3961 cgccagcagc gcgcaggcat gggcgtggcg caccgcctcg tcccagccgg ccgtcagccg
     4021 gggatcgccg gccaggacgc gcagatgcag gcggtgcccc tcgatttcca gctcgccttc
     4081 gagcccggcg gcgcgggggc ataccgccgc caggaaggcc ttgcccagct cctcgtgcag
     4141 gcagagcagg agaatctggg catgggttcc cgggaggagc ggcgcgggcg acccgctggc
     4201 tgtttcgtcc gtcatggcag cttgctctct actcccatcg ggcatcgggc gcaagggaaa
     4261 aaacgtttaa ggaaaagcgc ggggccggag ccttgctccg ccgggcgcgg cccgcgccgc
     4321 gcccgcgagc ccatgcttca cctcccgggt cccgcaggat tcgccgcacg cggcacagcg
     4381 gtactcgtgc aggtcggcct gcggcaggac cagcagcagg cgctcccgca ccggccgcgc
     4441 cgcgcggcac cgggggcagt acaactccgc ggccgtcagg ttttcaaatt ggcgtggcat
     4501 ggcggtcgca atctaccagc cccgcgcgcc ccctgcaagc cgcgcggcca aacaggactt
     4561 gtcagcccgc ggccggttgg tatagaaggc cccccgtgaa ctgcacccga tggacaatgg
     4621 agacccggcc atgaacgttc cctccgatct tcgctacacc cgcacccacg aatgggcccg
     4681 tctggacggc ggggtgctga cggtgggcat cacggatttt gcgcagcacc agctctccga
     4741 cctgacctac gtcgagctgc cgaaggtcgg cgacgaggtc ggcgcgggcg acgaggtcgc
     4801 cgtggtggaa tcggtcaagg ccgcctcgga catctacgcc ccgtgcggcg gcgtgatcac
     4861 cgaggtcaac gccgccctcc cggaccggcc ggatctcatc aaccgcgatc cgtacggcga
     4921 gggctggatc ttcaaggtgc ggcccgcctc ggccgacgag ttcgatgcgc tgatcgagcc
     4981 cgacgagtac gaagagctgc tccccggctc ggaaggctga tgtccgcccg cgctttcttc
     5041 ctggagttgc cgctgccgtt ctcggagggc gtggaactcc aggagcggct cgtcgaggcc
     5101 cgggcccgcg gggacattcc cgacaccgtc ctgtttctcg agcacgcgcc cgtcgtcacc
     5161 ctcggcgcgc gcggacggac ggagcacctg aagttgtcgc gggacgagct ggcggcgcgc
     5221 ggcatcgagc tggcgcccgc gagccgcggc ggggatgtga cctaccacgg gcccggccag
     5281 ctggtgatgt acccgatcct caagctgggc gagcgggagg ccgatgccca cggttatctt
     5341 ttcaacctgg aggaagtggg cctgcgtacg gcggcggact tcggcgtgca ggcctggcgg
     5401 cggccgggcc tcaacggcat ctggaccgag gccggcaagc tcgcggccat cgggttccgc
     5461 atccggcgct gggtcacgat gcatgggatg agcttcaacg tggatcccga cctgggcggc
     5521 ttccaagcca ttttcccctg cggcttgcgc gagcctgtca cctcgctccg ggccctgctc
     5581 ggcgggcgct gcccggccct ttccgcggtg cgggaccgca tggccgtcca ttttggggcg
     5641 gtcttccagc ggccgctggc ggtccggggc ggcaacccga tgccgtaaaa aagggcccat
     5701 ccggcggctt gacaggtccg gggggctggc ctatcctgtg cgcccttgtt agcagaagtg
     5761 ttgtcccggg cggcccgttc cggccggcga aacccttgga aagaggctct tcatggatgc
     5821 gtatgccgta gtggaaacag gcggaaagca gtacctggtg cggccgggcg aggtcgtccg
     5881 ggtggagcgg atcgcggcga aggccggcga caaggtggcc ctggcccccg tcctcgcggt
     5941 atgcagcggg caggcggcga cggtcatcgg cgcgccggtg gtcgagggcg ccacggtgac
     6001 ggccacggtc gcccgcgagc tgcgcgagga caaggtcgtc tcgttcaaga aaaagcgccg
     6061 caagggctac cggaagaaaa tcggccaccg gcagacgctc accgaactca agattgaagc
     6121 gatcaacgcc ccgtaacccg gacggcgctg gagggaaggt ccatggcaca taaaaaggca
     6181 ggcggcagca gtcgaaacgg gcgcgacagc caggggcagc gtcgcggcgt gaaggtgttc
     6241 gggggccaga ccgtcaccgc gggcagcatc ctggtccggc agctcggcac gcgcctcgtg
     6301 gccggcgaga acgtcggcat ggcgcgggac ttcagcctct tcgcgctgaa gccgggcgtc
     6361 gtccagtacg accgcaacgg caagcgcgtc aacgtccgcc tcgcggcggc gacctgacgg
     6421 tttcccgttc ctgtccgttt cgacaaggcg agccccgggc tcgccttgtt tcgtcatgaa
     6481 agccatcacc ttcatcgacc gcgcgcggct ctacgtctac gccggcaacg gcggcaacgg
     6541 ctgcgcctcg ttccgccgcg agaaatacat cccgcacggc gggcccgacg gtggggacgg
     6601 cgggcgcggc ggcagcgtga tcctgcgggc cgacaagaac gtcgactcgc tgctcgatct
     6661 ctactacgag ccgcaccgcc gggccgagca tgccgggccc ggccgcggga aacagtgcac
     6721 cgggcgcgac ggccgcgacc tgcgcgtgaa ggttccgccg gggacgctcg tgcggcacga
     6781 ggccgacggg gcgctcgtcg gcgagttgct ggccgacggg caggagctgg tcgtcgcccg
     6841 cggcgggcgc ggcgggctgg gcaacatgca cttcgccacg tcgtcgcacc gcgcgccgcg
     6901 cgaatgcacg cccggcacgc cgggcgagca gttcatcctg cgtctcgaac tgaaatccat
     6961 cgcggacatc gggctcgtcg gctacccgaa cgccgggaaa tccaccctgc tgcgggcgct
     7021 gaccgccgcc cggcccaagg tcgccgccta cccgttcacc acgctgcatc cggtcatcgg
     7081 cacgctggag ctcggcgacg cggagagcct gcgcatcgcc gacattccgg ggctgatcga
     7141 cggcgcgcac cggggcgtcg ggctcggtca cgacttcctg cggcatatcg agcgcacgaa
     7201 gctgctcgtg ttcgtcgtcg acatggcggg cgtggacggg cgcgatccgt cggacgactg
     7261 ggacagcctg cagcgcgagt tggagttgta caacgccgag ctcgctcgcc gcccgggcct
     7321 cgtcgtggcc aataagatgg acctgcccga ggcggaggag cggctgaaag acttccggaa
     7381 gaagacgaaa accaggccga tcccgatctc ggccctcgcc ggcgaagggg tggcgcgctt
     7441 gcgggaggaa ctcgtccggc gctgggaaac cctcgcgggc gcctgatccg gcggcgaagg
     7501 caggtgggcg ttactggact cgaaccagtg acctcgtgca tgtgagggac gacaggccgt
     7561 tttcgcattt ccatgaattt acgctactta ctgatatggc gataagttgc gcacgtatca
     7621 gaaaacgcca accgtggtca aatgcacaaa tcggatgcaa aaagtggccg cctccaaacg
     7681 catgtttttc ttccctattc gccgccgcct tgcattcctc cgcccgtgct ctaggtttca
     7741 accatgtgcg gacgctctac cgtcatatcg aagtttcccg agatccacga cgcttggcca
     7801 ctgcccgagg atgagcccga tctgaataaa ctcgtcccgt cgtacaacat cggccggatt
     7861 gcgtgcgcgg ggcgcgtccg tcgcgggcgg cgggctctac ttgtcctacg gcgggctggc
     7921 ccggtcgtgc acgatcagca acaacgtctc ggacgagacc ggcggcggcg ctaacctcta
     7981 ttacggcgga gccgctgttt ctgcccttga tgtcttcaag aagaaaccct acgagcccga
     8041 aaacagcaac accggagccc agcatctgga gcatccagta cgcaggctca tgatcctgct
     8101 gcatccaaat accgaaaacg caaaagacga gggccgcgac catacacatc acgaagctga
     8161 agacttgata tggggcagac gcgtgcttgg tttctgttgg atgccgggtc ttcacgaatc
     8221 gtttggcgac aaagtgactg gcgagcgggt ggaatgcttg atagcccaag aggactaaga
     8281 ccttgagacg agggaacccc agcgagaccc tgccgcctgg acagccttga ccgacgtcgc
     8341 cgatgacggt agtctgcttg cgcacaatcg gcgggccgct gaccgaaaga ggacaatcgc
     8401 gtatgacacg ccgtgtgggt tttctcgttt ttggcctgct gcccgccctg ctgcttcccg
     8461 ctgcaaaagc ggatatcacc tccgacctgc aggaaatcgc cgacggactc gtggatcagg
     8521 gcagtcccgc cgccctcctg catgtcgagg attccgctct cggcacgaac gccctggcgt
     8581 ccggcgtccg ctacccgaac gagatcatcg agggcgagtc cgtcacgggc gaactggccg
     8641 ccgcgcacac catgtggttc cactgcgcga cggtttcggg gcagtactac ggcgtcgtgg
     8701 tgaccggctt cacggctgat gtttcgctcc agcttgtgaa cgagtcgctc ggtttcgtca
     8761 ccaaccgctc gggcgggtgg accggctgga cgagccagga cgacggcggg tacgacgtcg
     8821 gcctgtcctc gacgggcaac acgccattcg ttttacgctt ctactccttc accaacacca
     8881 tcgcacgggc gacacagtgg atcacggagc agatggagat gcaggacgtg gttggccttt
     8941 cggtcgcgct ggttgacggg caggagatcg cctgggcgcg cggcttcggt tacgcggacc
     9001 tcgagaacgg cattcccgtc acgaccgaca ccgtgtttcg cgtcggctcg gtcgccaagc
     9061 tgttcacggc cacggccgcc ctgcagtacc acgaccagga cgaactaggg ctcgatgatc
     9121 cggtgaccaa ccacctgccg gggctccggc tgttgtatcg ttatccggag acggattcga
     9181 tcaccgtccg cgacctgctg gaccaccaca gcgggttgcc gggcgatcta ataaactcgg
     9241 gcttcaccac cgagccggtc gtcggacgat atgcctggct caccaactgc ctggtgacca
     9301 cgtaccccat ttatccgccg cggctgattt ttagctactg caattcgagc tttaccctga
     9361 tggaaggggt catcgaggcc gttgacggct ccggcgcacc gttcaccacc ttggtggcca
     9421 gcaacctctt cgagccgttg ggcatggagg ccacctcttt cttgaaggac aagcccgcga
     9481 tcgtcagcca gctcgcgaaa ccctacattg acgggcaggc catgccggag gaatacgcga
     9541 acatttacgg caccggtggg atgtacagcc gtcccgtcga cctggcccaa tacatccgca
     9601 tgattcttgc gggcggtgtg gcgccgggcg gaacgcgggt cctgggcacc gactcggtgg
     9661 cggaaatgct tacgccgcag gcaaccaatg cgccgttcgc tccgttcgcc ccgttaacgg
     9721 cagggctggg ctgggaggcc gtggaatggc cgccgctcaa gtatgcgggc aacctggcgt
     9781 tcaagagcgg atggaccatg gtctatcacg ccaacgtggc cgcgcttccg gagcgtgacc
     9841 tcggcttcgc catcacgctc agctcgccgg cctatatcga gaagacggcc acggacttcg
     9901 ttctgaagca cgcggtgagc gaccggtatg gaattcccgt gccgacgaac acggtctatt
     9961 tcccgggcgc gacgcaggcc gtgtcgcagg cggaactcga tgcattggcc ggcgtctatg
    10021 cgacggggga tggataccac cgggtggagt cgcaccccgg gtcgctgacc ctcgtgctca
    10081 acgccgaaag cgacgaggcg aagcgcttca ccaacatgct tctccgcgcc gacggctggt
    10141 tctcgccggg cgcctcgccg acgaacggca tgctgatcac gaacatcgcc gggcggacga
    10201 tgctcgtgtg gcggatagcg tacggcggat ccgtgttgac gaacctgcat tgtggcgagc
    10261 gcgtcaatcc cgcgccgctt tctgcggcat ggagcaatcg gctcgggaag gcctggtttg
    10321 tgctggatga atccccggcc ggctacagca ttgccctggg caccagcacg cgtctcacgt
    10381 tcaccaatcg cgacggcgtt ctgcttgtcc agtgcgacta cgtcgaaggg tcccaggtgc
    10441 tctcgccgac caacgacaat acggcctttg tcagcggcct gatcaatcgc ggcgactccg
    10501 ccgtgcaggt ttttacttcc aacaccttcg agtacctgcg ttattgcgga tacacgtttc
    10561 agccggcgga gagcttcccc gtcctgtatc cgggctcgat gaccaacctg tcgctcgcgg
    10621 ccggcgagac ccggtggttc gccgtgccgg gctggacggg cgacccgcag ggcctgacga
    10681 tcaccaatgc ctccgccccg ctgcggctta acctgtacac gcccgagggc tatttgctgg
    10741 gccgtggaac gagtgcgctg ccatggatct gtccgacggg tgggacgtac tacctctcgg
    10801 tcggcgcgat gaacaacgcc tccggcaatc tgcgcgcgta ctcgcttacc aacaccatcg
    10861 cgaaggctac cgagcagatc gaggggctga tggtatccaa cggggtggtc ggtctttccg
    10921 tcgcgctggt ggacggacag gagatcgcct gggcgcgcgg gttcggctac gcggatcggg
    10981 aaaacggcat ccccgtgacg accgacaccg tgttccgtat cggctcggtc tccaaggtgt
    11041 tcacgaccac cgccgccctc caataccgcg accggggcaa actggggctc gacgattcaa
    11101 taaccaacta cctgccgggg ctccgcctgc tggaccgcgg cggtttcccg ggcaccgatg
    11161 ccgtcacggt acgcgacctg ctcgatcacc acagcggttt gccgggcgat cttctgaact
    11221 cctccatgac tactgagccg ttcggcggac gctttgcctg gctcaccaac tacctggcgg
    11281 gcacgtatcc tgtgtatccg ccgcgcctcg tgaacagcta ctgcaactcg ggctttgtcc
    11341 tcatggaggg ggtcgtcgag gccgcggacg gctccggcac gcccttcacg gacctgatga
    11401 gcagcaacct ctttgaaccg ttaggaatgg acgcaagctc catcctcaaa gacaagcccg
    11461 acatcacgaa ccatctcgca agaccctatg tcaatgagca ggtcatgccg gaggaattca
    11521 taaatattca cggcaccggc ggaatgtaca gccgtcccgt cgacctggcc caatacatcc
    11581 gcatgattct tgcaggcggt gtggcgccgg acgggacgcg catcctggag accaactcag
    11641 tggcggaaat gcttacaccg caggcgacca acgcgccctt ggagccgttc gtctgggaga
    11701 cggttggact gggctgggac accgtacgat cgccggaact cagttacgcg ggcgacctgg
    11761 cctggaagaa cggcataacg accgtctatc gcgccaacat ggccgtgctg ccgaaccgcg
    11821 atctcggctt cgccatcacg gtcagctcgc cggcggcttt cgcgggcggg cctctgtact
    11881 tcgttctgca gcaagcggtg agcgacaagt acgggattcc cgtgccgacg aacaccgtgt
    11941 atttcccggc tgcaacacag gccgtgtcgc aggcggaact cgacgcgctg gccggcatct
    12001 atgcggcggg ggacggatac cacagggtgg aatcgcagcc ggggtcgctg accctcgtgc
    12061 tcaacgccca tagcgacgaa ccgccgccgg acggctcccg cgtggtcgtc accaatttgt
    12121 gtcttcgtac cgacggctgg ttctcggtgg gttccccgcc gtcgttcagc atcgtgatca
    12181 cgaatatcgc ggagcggacg gcactcgcgt accggcaggc gtcgggcgcc ctttcggaaa
    12241 cgagcctgat tggcgaacgc ttcaatcccg agccgcttcc cgcggcgtgg agcaaccggc
    12301 tcgggaaagc ctggtatgtg ctggacgaat ccccggtcag ctacttcgtt gtcatgggct
    12361 ttagcccgcg tctcgcgttc accaaccgcg acgcccttct gcttgtccag tccgaataca
    12421 ccggagggtc ccatgtgctc tcgccgacca acgacaacgt ggcctttgtc tgcggcctgg
    12481 tgaacgcgga agattccgcc gtgcaggtgt tcacgtccaa caccttcgag tacctgcgcc
    12541 tcgccggata cacgtggcag cccgaagaca gcatcccgga actgacgccg ggcgtgccgg
    12601 tgagcggcac caacctttcc actcaatgca actggtaccg cgctgacctt ccggtcggca
    12661 cgttcacgct gacctgcagc gacaccaaca tccgcatgat cgtctacgga ggaatggccg
    12721 cgccggtcat agccatcggt tcgtacacct ttgcgcagac ggcttccgga tccgtctatg
    12781 tcgcgctggg cacgatgtcg tcgtccgtgc cgtacgaact cgatgcggga cgcgccaccc
    12841 tcatcctcga acggatcgag ggggggccgt ccggcatggc gttcacatgg atcagccaac
    12901 cgggcaccaa ctacacggtc gaagtggcca ccaatctctg cggcgaccct gcgtttgcgc
    12961 cgctggcgag caacctcccg tcctcggacg aaatcgtgac cacgtggacg gcggccccgc
    13021 cggcatccgc ccttgagttc taccgcgtgc gtctcccgcc gtaagcgcgg aatcatcttg
    13081 acggccggag gccgcaggag taagcaggga tttaatcgcg gggggctttt gagcaggcac
    13141 tcgaatgcag gccgctgggg agccgtggtg ctggcgattc tggcgctggg cggcgcggcc
    13201 caagcccagt gggtcacgca aaacatttct cttcagccgg gctggaacgc ggtctacctc
    13261 gaggtccagc cggcgccttc cgattgcgag acgatcttca gcggccaccc ggtcgaggat
    13321 gtctggttgt ggaacaaaag gttctccccg gtcgagttcg agctggatcc ggatacgccg
    13381 ctggccccga atccgcactg gctgatctgg ctgccgtcca ccaacacgga atccttcctc
    13441 tcgcaagtct tcggcctgca ggcctgccaa tcctatctca tccgtgtgcc gaccagcgcg
    13501 gcgcccttcg agatctcggt gaagggggcc ccggttatcc cgcacctgga atggttccca
    13561 cacgccctga atctggtggg gtttcccatc aacccggtcg ccccgccgac gttctccgaa
    13621 tttttcacgg ccgcgccaca ggtggacacg tcgcgcggat tcgataacga gctgtacacc
    13681 ctcgacgcct cggggcgtgg gcgcacgatc gtccagccgt accgcgaccg gatccagccc
    13741 ggccgggcct actgggtgaa gtgcagcggc gcgctggatt acgaggggcc gttgcgggta
    13801 acgaccacga tcgccggcgg gctggatttc gggctggtgc tggaagagat cggtttgtcg
    13861 gtcgccaatg tctcgaccac cacggcggtc acggtgaccc tcaagcaggc ggcctccgag
    13921 gcgccgccgg ccggccaggc ggagctggcg ggcccggtgc cgctctccta tatgatcagc
    13981 accctcggca cgagcaacga cctggcgtgg acgaacctgc cggcgaaagg gctcacgaaa
    14041 accttggccg cgggcgagac gtggagcctc aatttcggct gccgccgcaa cgacatggcc
    14101 ccgtacacgc ccgcggggac caacggggcg gcgtaccaga gcatcctgga gattgccgac
    14161 gggtcgccgt cgctcctggt ccgcgtcccg gtcatcgcgg agaaggacaa cgtgcggcgg
    14221 ctgggattga ggcaggatga gtacgacctg gacgaccatc acagcgacga gggcctttgg
    14281 gtcggctacg cggtcatcaa cgaggtcacc tgcccgtcgt actcctccac taatctcctt
    14341 ccgacggaat ccgactgccg cctgcgcctg atcctgcact cggacgccta cggccgggtc
    14401 aagctgctgc agcgcgttta cctggcgtgg gtcggccccg cgacgaacgg cgagtacggg
    14461 ctcttcatgg acgaggcgtc cgtcccgacg gactcggagg acgtggacct catcagcagc
    14521 gccgccttcc cgctgatggc gccggtagcc ctggccaact gcgacaccaa ccggcccccc
    14581 acaggcctga cgaaccagct ttgcgggacc gtggtcctgg ccttcaacga ccccaccaac
    14641 ccgttcctgc accggtacca tccgctgctg gacaacaagg actggaactt ccattcctac
    14701 tccaacgccg tcgaatcgcg cgacgtgacg cgggaaatcc tcatggattt caacgacaac
    14761 gttttcaccg gcggcgcgcc ggacccgttt tacggcatca gcgaaatcgg cgggacctac
    14821 gaggagatcc tcaccggact gcgcgaacag ccgattcatg tgaaaggggc cttctatttg
    14881 cagcgggtga gcctggtgaa cgagttgcac tgaaggtcgg gcgcgttggc tccacgcgcc
    14941 gcgcggaagc ggatttataa gaagggacgt gattatgaga acgaagaaaa gaaatccatt
    15001 cctccttcat ccctcatccc tcatcctcct cctctgttca tccttcatcc ttgacccttc
    15061 atccttctcc ttcgcccagt ccggcgcgcc ggccgttatt aactaccagg gccggttgta
    15121 cgacccctcg ttgggcggga cgggcgggcc gctgaccggc gtgcagcaga tcgagttccg
    15181 catctacgac aacatcacgg gcggcacgct gatctgggga cgccagttcc cggtaagctg
    15241 cacggaccag ggggtgttca acgtgctgct gaacgacggc ggcacgtggc tggccggcgc
    15301 gaccaatcac ctgccgtccg tgttcctggg atcggatcgt ttcctggagc tgaccgtggt
    15361 cggccacggc agcgcgatca gtccgcgcca gcagctcgtc agcgcgccct acgcgttgca
    15421 ggccgcgcac gcggcggatg ccctcgcggc gggcagcggc ttcaccgtga acggcggggt
    15481 cctggccgtg aacggcctcc tgaacatgag cgggctggcg accctcaaca gcggcttggt
    15541 ggttcgcggt tcgaccgcgc tggccggcac cctcggcgtc ggcggggcga cggtcgtcag
    15601 caacaccctg catgtcaccg gcaacttcga cgtggccgcc ccttcgacga tcgcggggtt
    15661 cgggagcatc ccgatcggtg gcatcatcat gtggtccggg gcgcccgcgg gcatcccgtc
    15721 cggctgggcc ctgtgcgacg gcggcacgca cgagggggtg ccgaccccgg acctgcggaa
    15781 tcggttcgtg gtgggcgcgg gcggcgccta cgccgtgagc agcacgggcg gcgtggacac
    15841 cgtgacgctg gaccagcagc agatcccttc gcacacccat cccgtgtacg cgcccaataa
    15901 cgatgtcaat gggagcacat cccagggctg gccggccaac aacaatcacc agtcgttccg
    15961 aagctccgac cggggccggg aatacacgat caacagtgcc gccgtgggca gcatcggcgg
    16021 cgggcaggcc cacgagaacc ggccgccgta ctatgccctt tgctacatca tgcgcgtgaa
    16081 atgaactcat gagccggatc gatagaagcc gtttccaaac actggaaaaa tcgggcgcga
    16141 attttccaat ggtggggaaa aaacgggccg cattttccaa tgtttggaaa acgggctgcg
    16201 cctggctgtc ggtcctggcg ttgcccgccg cgggggccca gacgccgcag ctcacggcgg
    16261 agttcctcaa cccgtggagc gtgcccgcgc cgggcggact gtactccaac tactacgtca
    16321 acggcgtcgg cgcgccggcc ttcggcggca gcacgttgac caacatcctc accgcgccgg
    16381 acgggtcgtc cagcaccgtg acctaccgat ccccgtccac cggcctggtc ctcgccttct
    16441 cgcagtacgg ccagccggtc gagtccgatg tggcgcagta tgacctgggc gacgtgatcc
    16501 cctggccggc ggggatggtc agcacgagcg cgccgccggt cgggttcgag gcggtccgcg
    16561 tgggcaacaa caccaatgcc tactaccaga tcacgaatcc cgtcggggcg gtgatctacc
    16621 tcccgtccac ccgccaggtg gtcgcggcag cgggggggaa cgtcgagatc ggctggctga
    16681 cgacgggggg ggtggtgcgc accgtgtaca ccatctccgc ggtgccggcg gagcggccgg
    16741 cgcgggtgtt ctggacggag ccgccctaca acgcgccggc ggtgaacctg agcgcgaacg
    16801 gccagcaggt cttcgcgtgc atccactaca acaactacgt cacccgcccg gtcatggagg
    16861 cggtcacgac ccagaacgcg cagggggagt gggtcgtgac gacgcagctc gtcagcggcg
    16921 tctggctgga cggcgagggg gtggcgcaaa agatccgcgc gatggacgtg gaggggatcg
    16981 cgctgctcga gtactacgcc gacggcacct tcacccggtc cattgatctc cagccgatcc
    17041 aggtcatgcc gccgaccgtg aacatcatcg aggcggacat cggctcgcgg ctgttgccga
    17101 cggaccatta ctacggggtg gacgatttga tcccgcggat catggccggg gcgggggacg
    17161 acggccgcgt cctgaaggtg ggcctggacg ggccgaagga aaactgggtg ttcgccatcc
    17221 ggcgcacggt ggaggatccc tgggacgtgg aaatcttctg ggagcaccag gacgaccggg
    17281 gggtggtgtg gccgttcgag gtggactggt acgcgctgga ctggccggac cacgcgaccc
    17341 ggtacgtcct gggcgaagcg ctgacgaaca ccgcgccgat cttcctgccg gacagcctcg
    17401 ccgcggccct gcccaacgag gagccggcgg gcatcgcgtc cgtcgccgtg gacggccgga
    17461 gcttcagcgc caccgcgccg gggcacatcc tcgtgcaata ctcgacgcac gacaacatgt
    17521 ggttcgacgt cctgcgcgcc gtgcggcacg acgatcggac ggagtacgac ccgaccccgc
    17581 aactgtggga catcggccgg gagatcctgc ccttcgccga cgacaactac gtgctgtact
    17641 ttgacggcac gaacgaatac gtcgaggcga cgatggatgc cgggttccac gggcaagacg
    17701 ggttcacggt cgaggcgtgg gcgtaccggt cgggacggat catgaacaag gatgggcccc
    17761 tggtgtcgca cacggcatcg gagggcttcg ccggttcgaa cgactgggag atcgcgtacg
    17821 ggtggggatc ggacgtgtat ttctggacgc acgccgggac ggcctcatgg agcatgtccg
    17881 gcaccgccga cctgaaccag tggcaccatt tcgccggcgt gtatgacggg accaacgtgc
    17941 tgctgtacgt ggacggcgtg ctgcgcgcga gcacggcggc gtccacggcc ctgggcgtgg
    18001 cgaatcgccc gctgatgatc gggcactcgg tccgccagaa cgcctatttc cagggcaaga
    18061 ttgacgaggt gcggatctgg aacacggcgc gccgcgccgc gcagatctcg gaccacatgt
    18121 accgctcgat ccagggcccc gaggacgatc tcgccgcctg ctacccgctg gacgaaggcg
    18181 cgggaacgca catccgggat gcggtggacg ggtcgtacgg ccagttccac ggaacccccc
    18241 agtggtcggc ctcgttccac ctggccaaca ccaacgtcag cgaactggcc gagttccccg
    18301 gctacatcta ctccggccgc gcgtacaacg tgaaccgtta ccactacccc gacgaggacg
    18361 accccggccg ggcgtcctac atcttcgcgg tcaacaccaa cgatttcgaa atctggtggg
    18421 ctcggccgag cagccagacc gacctgccct cccaagtcta ttatccctcc ctggccgtgc
    18481 actaccacgc gacgtggccg accaacgcgc cgcagatcgt gatcgccggc ggccggggga
    18541 gcggcgacga cgtgttgagc gcgccgtcga tctactacca gaacgacggg gcgttgcccg
    18601 gctacaaccc gaacgaggag cacgcgctgg tcctgggcgg caaggcctac gcgctgcggg
    18661 acgacctgaa ccggaccaac agcagtcagc cctttgtcct cgtggacggc ctggacccgc
    18721 agacggcgcg gcccaccatg aggacgtacg gcgtggtccg gaccaacgcg gactatcgtt
    18781 tcgagtattc ggtgaaggcg ggctcggcga tcatcccgcc gatgccattg agcgcgatgc
    18841 cgccgtgcag caatacgacg tacgcctggg ggccggcgtg ggaggaccgc aaggaaagct
    18901 ggtgggccaa ggcggcgggc aacgacgggg gcgacacgaa tgccggcatg cggttcttct
    18961 accaggtcca gccctcgttc tggttcccgg ccctcggcgc ggggagtcag ccggccctcg
    19021 ggcgcgaggt tccctggctg ccgaccaaca gcccgccgct gcagggcgga tcgtacggca
    19081 cgccgctggc catgcgctac gacatctcgt ggcccgccga ccggccggag atgcagatcg
    19141 cgcagaccct cacgctggcc acgcgcggcc tgccggacat ctggaaccag ttgagcgtgg
    19201 atgtgattta ccagcagtcg cgcaccaacg ggcatggcga cagcgtttcg ttgttcgacc
    19261 cgatggtgga acatgccgtg gacctggata tctccgtgat caacgccatg gtctccgccg
    19321 ggctggcgcg gaaggacctg acctcgtccc ggtacatcct ctccggcctc gcgccctcgc
    19381 tgcgcagccg catgtattac gatccggacc ggggcgagag tggccagctg gtcctgggcg
    19441 gggaataccg caccacgctc acgggcggcg gctacctgct gccgaacctg ctcgacaccc
    19501 aggaccggga ggacgccatg accatggcgg atccgctggc cggcgacaaa aagaccgcct
    19561 gggtcagcgc cgtcaacgcc ctgcccacca acatcacgct gatcgtcccc aacgtcccgt
    19621 acgtcaaggc cgcgttatgc tccgccttga gcgcgcagtc gaacgccgtc ggctacgtga
    19681 ccctggtgtt caacaacagc accaatgccc agcaggtccc cccctcgctg ccggtcagtt
    19741 tgagcatcat caaggtcgtc ccggagctct acaacgcgtt cctggatgtc atcgagtcgg
    19801 acgacgcgct ggacgagaag ctgtcgctcc gctacgccgc cgactacgct gggcgcgcgg
    19861 aggagtacga cttcgactgg cgctgggccg agccggaggg cggcctgccg cccaccacga
    19921 actacgcggg ctggagcgtg tacggcgcgg gcatcgtgca gggcgagttg ggcgtcaaca
    19981 tcgagggctc cagcccgttc accctggcgg accactactt cgccgttcgg tacaagcgac
    20041 acgacgggct gggcccgacc gggaccaact ggagcgagtg gtcctacaac ctggcccccg
    20101 gctggatcca gcgggtgatg aatgccatca acccctacga gcagcgcctg cacgacatga
    20161 tcgggaacag cccgaacctg atgctgagca tgatcgggca ggcggggccg ccgtacgagg
    20221 gcgcggtcgc cctgaacgac gacgccgtca actcggccgg cctgatccag atctaccaga
    20281 ccgtgctgga ccgcgcgatc tccatgagcc tggacgccgg catggacgac acggccaaca
    20341 acgacgccct gctcttcgcc gccagccggc tgaacgacct ctacatgctg ctcggcaacg
    20401 aggccttcgc cgacgcgcag gacccgacca tcgggttcgg caccgactcg acctggttcg
    20461 agcactacgg cgcggcgtgg tcgtcgctgt tctgcttcat gaaccagttc ccgaccctgc
    20521 tcgacgagga actgggcctg ctgcgcggcc gcgacgacac gctggagccg tccacggagc
    20581 tcacgccggt cttcaaccgc ctgatctgga actacacgcg cgggatcagc ggcggcgagg
    20641 tggcgtacgc catgagctac aacatcaagg gcgaccccac cagcacggtc ggcgtggtca
    20701 ccgccgagga cgccaaggcc ctctacccgc agggccacgg cgatgcctgg ggccactacg
    20761 cctgcgccct ggcgggcttc taccgcctgc tctccaacac caacttcggc tggaacacca
    20821 ctcccggcgc gacgctcgtg ggcaatgccg cggtcagctc cgacttcttc gacgagcaga
    20881 agttcgccga ggcggccgcg gcgaaggcgc ggaccggggc cgagatcgtc agccgtacct
    20941 accgcaagca ctacacccac gcgaccggcg gccgccgttc gttctgcggg gacagccaaa
    21001 cgaaccgcgc gtggggcgtg gacggctggg cctcgcgggc cgggcagggg gcctacctcg
    21061 actgggtcgc gggcaactcg ctgctcctcg acaacctgac gaacctcgtc caggtcggcg
    21121 gcggggacca gccgcccgag ggcatccagc ggatcgaccg caccaccgtg ccggagttgg
    21181 acgatatcgc cgcgaacctg gagcagatcc agaagcagtt ggatcacgcc gacctcggcc
    21241 tgagtccgct cgggttggac cgggacgtgg tgcccttcga catcagcgcc gccgagattg
    21301 acgccggcca gacgcatttc gaacagatct acgcgcgcgc cctgggcgcc ctggacaacg
    21361 ccgcggcggc cttcgactat gcccagggct gcacggagaa gctgcgcgag cagtgggatt
    21421 ccgtgtccga cttcaccggc gccgccgccg agtcggaggt ggactaccac gaccgcctgc
    21481 tggagatttt cggctatccc tacccggacg atatcggtcc cggcaagacc tatccccagg
    21541 gctacgccgg gcccgacctg gtgaactatc agatcctcga tctcgaggat ctcctgggcg
    21601 tcgccccgac aggcggggcc caggaggtgg tgaccatgta ccgggtgacg ttcaccgccg
    21661 acaacaacag gcatgactac acctacacca cgaacttctt caccaacacc accctctacg
    21721 tgaatgacaa ggggcttaag gtcaagccgc cgagctggac cggccggcgc cccgcgcagg
    21781 gggatatcca gtccgcgctt tcggacttcg tgaaggcgta ctacggcttc ctcaaggcgc
    21841 gggacgagta cgcgaacaag atggccggcc tgtccttcgc gtacgagcac ttccgagata
    21901 ccgcctcgcg cttgagcaac gaatggctgg tggcgaccca ggttgcggac aagaagaaaa
    21961 cgctggcggg ctggatggcg gccctggaga tactggccga gctctcggag attggttcga
    22021 gctacggcga gaacatggcc aaggccgtgg agaaagcgct gcccgacatc gagatgcaag
    22081 cgtttcctcc ttccgtaaac gtctcggtca gcatcgcgaa ggcggccgcg gaagccggta
    22141 attccaccgt gaagttctgg tgggaagtgg cgacccacgg gataaagagc tttgccgcct
    22201 tggggaaggt tcttggcgag aacgccgtca tagatctgga gttgggcacc gccacgaacg
    22261 ccttgagttc ggagatcctg tgggggtcgc gcgaggtgga ggagaagctg cgcgagcaga
    22321 ccgtggtggc ggacgagttg atggcgcaga tcgaggaaat gtgcgcggcc gagcagcagt
    22381 accttgcggt cctcaagaaa ggagagcggt tgatctccga gcgggcgcgc ttccgggcgc
    22441 gcacggcaca gcgcctgcag gcggaccgct acgccgacat ggccttccgg atcttccgcg
    22501 acgaggccct ggccaagtac agcgccacct tcgatctcgc cgcccggtac tgctacctcg
    22561 ccgccaaggc ctacgactac gagaccggcc tgctgggaac ggataccgag cgctcgcccg
    22621 gaagcctttt cctctcggac atcgtcaagt cgcgaagcct tgggcggatg caggacggca
    22681 cgccgctggc ggcgggcacg gaaggcgacc cgggcctggc cgacgccatg gcccgcatga
    22741 aggcggactg ggacgtggtc aagggccggc tgggcttcaa caccccggac acggagacga
    22801 gccgcttctc gctgcgcacg gagctgttcc gcacctcgcc ttccacgtcg agcgacgaga
    22861 cctggcgcaa cacgctggag ctgaagcggg tggacaacct ctacgacctg ccggaattcc
    22921 aggagtactg catcccgttc tcctccacga ccaatcgcga gcccgcgatc gtcatcccgt
    22981 tctcgagcta tgtcctgtcc gggcacaact tcttcggcaa gaaccttgcg ggcggggaca
    23041 acgcctacga cccgacccat ttcacgacca agatccgatc cgtgggcatc tggttcacgg
    23101 ggtacaacgt atctttcaac accaacctgg tgaccggcgg cgggctggcc aacgagcccc
    23161 gggtctacct ggtcccggcc gggcaggata tcatgcgctc gccgacccgc ggctcagaga
    23221 cctggcggac atggcgggtc ttcgaccagg ccctgcccct gccgttcgat atcggccgcg
    23281 cggagttgga cgatcccgac tggatcccgc tcttcgactc gctgggcggc accctggccc
    23341 agcggcggcg gcacgcctcg ttgcgggcct accatgaccg ggggcagttc gacgcgtccg
    23401 agacctgcag caacgcccgc ctggtcgggc gctcggtgtg gaacacccgc tggttgctga
    23461 tcatccccgg tcgtacgctg ttgtccgacg ccaacgaagc catcgagcgt ttcatctacg
    23521 gggccaagct tgccgacggg acccgcgacg gcaacggtat caaggacatc aagatcttct
    23581 tccagaccta ctcgatcgca ggtgaataat gaccgcgaaa agacatgtcg agcggaacgg
    23641 ggcctggaaa ccaagccggt ttcttctgct ctgcatccta gtatccagca tccagcatcc
    23701 agtatccacc ttcgccggca tgccccagcc catggtcgtg tactacggcc aggccaagga
    23761 cgagtatggc ttgccgtacc ggaacggggc ggacgtcatc ctccggatcg gcacgaacga
    23821 gatcacacgc cacacgatca acggttcgat atcgccgggc gtgaacttca cgctgtatgt
    23881 gcccattgat gacgggcgcg acgacacgcg gtatgtctcc tacgcggcga ccacaggcca
    23941 gtgcgtgacg attgtggtcg tggacgaccg cggcgagcgc acgatcatgg agggcgccgc
    24001 ggtgccctgc atcgagcatc ccggcgacct cctgcgcatc aacgtcaccg ccgggatgga
    24061 cgcggacatg gacgggcttc ctgacgagtg ggagtggcag ctgctcctgt ggggcgataa
    24121 cccgctctac cataccgtcg aggatattgt aggcagcgat gattacgacg gcgatggtca
    24181 gcccaacgac gacgaatacc gggcggggac atttgccttc ctggattacg attacttctt
    24241 tgccgagcgg cttgcccgga cggcgaacag tcgcttgcgg gttgagttct taagcgtggc
    24301 gggaaaggtc taccggatcg agagcgcccc gttcgaactg gccggatggt cctttgcctg
    24361 gcttccttgc gaatacgccc tgacggacac gggcgccctg cagaccggtc cggccgaagg
    24421 cacggggaac tggttctcat tctacgtgcc cgacgtgccg tcgaatcacg tgctgcggct
    24481 gaaggtggag tagcgctgcc ttgccgcaag gcggttcgaa gggtatacat taacactcat
    24541 gaacttgggc cggttgtgtt ggatggtgag cgggttgatg gccctcgccg cccattctgc
    24601 catggccgat ttggtggcct ctgacaacgc cgacaacgat ccgtatcccg acgggtggaa
    24661 tccgggcgac aacgggggct ggggttcacg gcctgggtga atcttcaggt cggcgatctc
    24721 ggctcgatgt atgtggaaca attcgctccg cttgacgggg acggttcctg gggcttgagc
    24781 gacacctacg cattggggcg gggattgtcc aacagtctcg cggtcggtca atggacattg
    24841 ctggcccggc acgggtccga cgtgaactct ttttgcggat tcaacctgcg cacgtccacg
    24901 tccacggagt cctttaaaac ggatgaaatc ctccgcttcg ggatcaacta cgatgaagga
    24961 gccaacgcca tgcgggtttg tttctccacc aacgccgacg cagactatgc atatcttgat
    25021 ctaggtgacg acgacgtgcg ggatgccgat ctggaataca gcattgcctg gtccaccgtg
    25081 gcgggttcgt ttacgctggg cgtggggaac ggcaaggact atgtcgaagt caccgcggat
    25141 ctgccctccg gcagtcccgt ggccatgctg ggcgccggca ttttcgagat gaacctggac
    25201 gataaagatg atctgacctt cgatgactac ggcattagtg tgatccccga gcctggcgcg
    25261 gccgggaccg tcgtactggg cgcctggctt ttgcagcgcc tgttccggag tcggattcgc
    25321 agaacctccc ggcacgttct ccaggtttaa ttatttgctt cattgcgcta gcgaccagct
    25381 gatgagaccg gtttagaacg gaaaccgggt aacactccgg aagctacggt tcgggaagaa
    25441 ccgggaagga gtgttactcg atgaagagga agatgaaccg atgcccccaa cgaacgctgg
    25501 agcatggatt tcgtgacggc gaggctggag aacgggcgtt acttcagagt gctgacgctg
    25561 gtggatcagt acacgaggga atgcttggct ttagaacctg ccttctccat gaacggagtc
    25621 aaggtggccg cctgcctgga ttcggttgtc gccgatcggg gccggcccaa atccatacgc
    25681 gtggataatg gaacggaatt ccagtcgcga gccatggacg gatgggcata ccgccatgga
    25741 gtgcagctgg attttatccg gccaggaaaa ccagtcgaaa acggtttgat agagagcttc
    25801 aacggaaggc tcagggacga atgtctgaat gtgcatctat tctggacaat cgaggatgca
    25861 aaggagaagt tggtggcgtg gcaggtcgat tacaaccaga accgacctca tggatcctta
    25921 ggacaactca cgccaaatga gtatgcggaa agagcccgga aacaagagag aatgtggcct
    25981 ttggagggag aaatcctaaa ggtctagcct gtccagtttt tgggagagga ccaaggccga
    26041 gaccggtgct gacgtcttga ccctttcaca gcttataggt cactcgaacg tccggacgac
    26101 ggcccgttat gtggcgacga attttgaaca ccctcggaag gctatcgacc gcgtgacatc
    26161 cacaatcgtc caactgatgg aatcggcaga aagcgggaaa aaagtggcca gtaaaatggc
    26221 cagtaaaacg acggcaggat tttcagagcc gggaagaggc tgtgcaacca gcgttaaaga
    26281 agcgagttaa ggtggtgggc gttactggac tcgaaccagt gacctcgtgc atgtgaggca
    26341 cgcgctctaa ccaactgagc taaacgcccg ggaaacaagc gtactttgtc cggttcccgt
    26401 ccgggtttca agcggaaaga cggcgggacc cagggccgct atcgcaggtc gggatacagc
    26461 ttcttctcgc actccgggca gatggaatgg ctgaactcga cgtggatgcg gtcgcgcatg
    26521 tagctctcga tctgctccca gtacccggcg tcgtcgcgga ttttcttgca gttggcgcag
    26581 atcggcagca tgccgcgcaa ctgccggaca tccgcgaggg cggccttgag ttcgccgagg
    26641 atccgttcgc gttcctgctc ggctctcttg cgcccggtga tgtccatgca gacgccgtag
    26701 aggtgccact tcttctcccc gagcttcacg atgcgcgtgt cttcctggaa ccaaagcgtc
    26761 gagccgtcgc gggccgcgca cacgaattcc tgccggtact cgcccgcccc cgaacggatc
    26821 tgcccggtgg cgaactcgcc catgcgcagc gcctgctcgc cgccccggcc gaagtaccag
    26881 gcgtggatgc ggaagttttc gccgatctca gagatgtcca cgaattcgcc gcgctcctcc
    26941 gcgaacaccc gcattttcca gaccagctcc cggccgccgt ggtcctcgac ctcggcgtgc
    27001 cagaggatgc actgggcgct ccgcgcgacg aggctcaact cgtgctcgct ccctcgatgg
    27061 gccgggcccg tctcttctcc ctggccgttc atggcgcaga ctatgccacc cccgaaaagt
    27121 gtcgacaagc ccccatcacc gcctcgattc cgcggacccg cggcgctaca ctgggcccca
    27181 tgcaggtcct gatgaaaaac cagaccgcgg agggcttgcg cttgcggctg gagggggtgg
    27241 gcctgacggc ttccgcggcc cggcggatcc aggccgcggt cctgcgtcgc ggcgtcttcc
    27301 ccggtccggc gcccggcctg ccggccgccc tgctggcctc cgtccgggag cggagcgaga
    27361 tcccccgcct ggcgctcctc gaaaagaccg tctcgccccg tgacgggttc gcgaagtacc
    27421 tctttcgcgg cgccgggccg gagcccttcg aggcggttcg catcccgctg ctgcaccggc
    27481 cggacgaccg gaagtacgtg gcctgcgtca gctcgcaggt cgggtgcgcg ctgggttgcg
    27541 cgttctgcgc cacgggccgc gccgggttcg tccgggacct ggcgccctgg gaaatcgtcg
    27601 accaggtgct gcacgtccgc gacgactcgc cgcatcccgt ccgcggcgtc gtgttcatgg
    27661 gcatgggcga gccgctgctg aactacgacg ccgtcatcgc cgcggcgcgg atcctctccg
    27721 agccctgcgg gccggcgatc gcggcgaagg ccattacgat cggcacggcc ggcatcgtct
    27781 ccgcgatccg gcggttcacg gcggagggac atcccttccg tctcctggtc tcgctgacca
    27841 gcgcggaccc ggcgctgcgc ggcgcgctga tgcccgtgga gcaggcccat ccgacccgcg
    27901 acctggtcga cgcgctccgg gagtatcacg ccgccacggg ccggcgcgtg acgctggcct
    27961 ggacgatgat ctccggcttc aacaccggcg aggccgacgc gcagcaactg gccgacctgg
    28021 cgcgcggcct gccggtgaag ctggacctga tcgacgtcaa cgatccgtcc ggccgcttcc
    28081 gcccgccgga tgcggaagaa ctcggcgcgt tccgcgacgc cctgcgccgc cacctcggcg
    28141 cgcccgtggc ccggaggtac agcggcgggg cggacatccg cgccgcctgc ggcatgctcg
    28201 cgggttgcgg aaccgcgaag atgccgtagg ttcctcctcc gggccggatg gcccgaagga
    28261 gaacgccatg aaaagccgcc tgccgatagt agtcctgctg cccgtcctat tctgccttcc
    28321 ggccctcgcc gaaatccagt cccgcgcggt ggaataccgg gccggggacg ccgtgatgga
    28381 gggctacctc gcgtgggacg accccgtgcg gggcgcggcc tacaacgaaa aggccgaccg
    28441 acgctcgtgg cgggccatgc ggaacttcct cgaggaaacg ctcggccgtt gaccgcgccg
    28501 tcgccgggaa tcacggggcg gcgggcgcgg atttgtcgat ggccttgtcg ctgacttcga
    28561 ccgcgaccag gcagatgtcg tcggcgacag gctcgttccc gacgaacgcc agcgcggcgt
    28621 ccatgaggcc gtccacgacg gcccgggccg gctggtacat cagcttctgc agcgccttct
    28681 ccatccgctg gatgccgaac tcctcgcccg cggcattgtg ggcctcgaac aggccgtcgg
    28741 tgaagaagac gtacaggtcg ccgtcgttca gccggcagta gccggccgtg taatcctcct
    28801 gggggatcac gccgatcgcc gcgccgcgcg gcatgggcac gtccagccgc tgcagccggc
    28861 cgaggccgcg gtgcgcatag atgggcgcgg ggtggccggc ggaggcgaag gtggcgacgc
    28921 gcgaggtcgt gtccgccacg aagtagaacg cggaggcgaa ctgggtgccc gggatgttgg
    28981 tcagcagccc gcagaactgc cggttgagct ccttgacgaa atgccgcgcg ttgcggccct
    29041 gcggcgcgag atcgtcgatc agcgtgcgga tgatcgcggt gatcagggcg ctgcgcgtgc
    29101 cgtggcccat gacgtccgcg atgaagatgc cggcgcagtc ggaggcgagg gtgaggatgt
    29161 tgtagaaatc gccgccgagc gcggaggcgg gctggtagcg gtgcgcgaag gagaggcgga
    29221 ggtggccggg gacgtggacc gcggggatcg cggggtaggg gcggtccatg taggcgagct
    29281 ggaaatcgcg cgccaggtcc aggtcgcgct ggatctcgaa ggactgggcc tcctggcgct
    29341 gccgcatctc ctcgaccatg cccttgagcc agtcgtccag gttctgctgc acgggagagg
    29401 gctcgcccgg tccttccgtt tcgtcggcgg ggacccggcg gcccatggcc cggttcagcg
    29461 cccagttccc ggcctgcagg acttcctgct gctcgccgcc gtagaaaacg cggaagaggc
    29521 gcgcgcagat caacgtccag gcggcgccca gaacgaggac cccggccgcg agaaccagcg
    29581 gcaggaggaa ggcgggagcg gtctccccga tgcccatccg ccgggccagc ccgccgccga
    29641 gaacggccag caggatcaag ccaagccagg ccggcgccac ggccgcccat cccacccgct
    29701 gcagaagcgc cttccggtat ttcatgccgc ggtgttatgc catacccccg gcggccgcac
    29761 aaacccgttt ttcccgcccg cgccccgtgg gggaaatggc gtcttgattc gggcaggcca
    29821 cgatggcata gtaacgacaa cagaggatgt ggaggggagg atcccgtgac agaaacacct
    29881 catgatccca agttggccgc cgccacggat atcattttca actgcccctt ctgcaacaag
    29941 agcctggcca tagacctccg cggcgcgggc ctgatggtga cgtgcccgga ctgcggccgc
    30001 gatgtgcagg tgcccgcccc ggcgcccggc gatacgctgg aggaggtccc gaccccgccg
    30061 atgaccgttt ccgccgaggc cctcgccgcc gcgcacacca agatcgagcg attggtcgat
    30121 agcctggagg aaatccgcga gcgccgccgt tacctcgaac atctccgcac ggagaacatg
    30181 gcccgcttcc agcagatcgg cggcgaggtg gaagtgatcc agaactcgct cgaccgcatc
    30241 gtcggcctgc tgcaggcggc tgccgccgac gagaaaaaga gcggcgagcc gccgccggaa
    30301 agttgagcgc ccgccgtccg cgcggcgcat caggacggta tccgcatcgt caccgtcgtc
    30361 cccgcgccgg gccggctgga tatctcgatg cgcccgccgt gcgcgtccgc gatggccttg
    30421 cagatggcta atcccagccc ggcatgcgaa cccgtgtcgg cgcgggcctt gtcgccgcgg
    30481 tagaaccgct cgaagagatg cggctggtct tcctcgggaa tgccctcgcc ggtgtcggac
    30541 accgacaggc tcgcggcgtt cgaatccggg cgcgtggaaa tccggatttc gccgcccggg
    30601 cggctgtaat ccacggcatt cgtgaggatg ttgacgagca tctggaccag ccggtcggcg
    30661 tcgccgacgg cgggtgcgga agtaaaatcg caggttatgc ctatgccttt tatttccgcg
    30721 agggggcgga cgaagtccgc gccggtctgc gccaactgcg ccaggtcgca gggggcgcgc
    30781 tcgagccgca cctggccggc gtcgaaacgg gccagcgaca ggagggattg gatgagcctc
    30841 cgcatctgcc gggcggtgtt ctggcaggtc tcgagggcgg cgcggtattc cccgggcgtg
    30901 cgctcgcggg cgagggtggc ctgggcttcc gacaggatga cggcgaccgg cgtccgcagt
    30961 tcgtgggccg cgtccgccgt gaactgcttc tgctgcgcga aggaagcctc gagccgcgcg
    31021 aaggcggcat tcagcacgga ggccagccgg cccagctcgc tctcggtccc ggccaggtcg
    31081 atgcgctccg cgaggttgcc ggacgagatg cggccggccg tctcgccgat ccgccgcagc
    31141 ggccgcaggg cgcgcccgac gaggagccat ccgcccccga tcccgaggcc gagcacgacg
    31201 cccccggcca ggatcagcca gccggcgaag cggcgcagcg tcttcagttc tgattggatc
    31261 ggcagcccgg ccagcacgcc gtccccgcgc tccgtgaaat agtaggcctc gcggagggcg
    31321 gtcctcgtgc ggtggcgaat tctcggatcg tcgcccgtcc aggcggggcg gacgatgccg
    31381 ggcggggcgt tactcgagca acgcagcggg gcgccgtcgc gcgaccacgt ggcgaagtag
    31441 aacccttcgg gatccgtctc gtcgaacagc gccttcgtct ggtcggaaag cccgaaggtc
    31501 cagggcccgg gcgggcctcg atggcgctcg cgtccgggcg gcggtccctt atccgggggc
    31561 ggcgggcgct cggaatccgg gccgggcggc ggcgggccgc gccgccgcag gtccaggctc
    31621 aagagcgcca tccggcgctc cagttgctgg tcgatgtcgc gcaggcggtg gagccgctgg
    31681 agctggtagg ccgtgacgcc gagccccgcg agcgccaggc cgaggagaat cgccagccag
    31741 aggagcatcc gccagcggat cgaccgcgtg aaccgtaagg cgctcatggc tcgatgcaat
    31801 acccgtgccc gcgccgcgtg gcgatgatgt ccggcccgag ctttttccgg atgccgaaca
    31861 cgtgcacgtc gatcaggttg gacagcgtgt cgctggtctc gtcgaaaagg tgcatgtaca
    31921 gggtgctgcg cgtcaccacc tcgccgcggt ggaggacgag gtattccagg atcgagtatt
    31981 cgcgggccgt cagcggcacg tcccggccgg cgcgcgtgac gaggcgggat cgggtgtcga
    32041 tccgcacgtc ctgcgcctcc agcaccggcc gcgccgtgcc cgccgcccgg cggatcaggg
    32101 cgcggaggcg ggagagcagt tccgggagat cgaacggctt ggcgaggtaa tcgtccgcgc
    32161 ccaggtcgag gccgcgcacg cggtccttgg tcccgtcgac cgcggtcagg atcagcaccg
    32221 gggtcttctt cagcgcgcgg agccggcgga ggacgtcgag gccgttcagg cggggcagca
    32281 ggaggtccag gacgatcgcg tcgtaatcgt attcctccgc tttgccgagc ccgtccgcgc
    32341 cgtcggccgc ggtgtccacg gcgtagcctt cctcgcgcag ggcccgcgcc aggttgttca
    32401 gcaaccgcgg ctcgtcttcc accaccagca ctctcatggc cgagtccatc ctctccgatc
    32461 ggggccgcct tatccagcgc cgaagcaggg caccctcaat cctccgccgg ctccgcgcgg
    32521 taaaaccgtt gggggaaggc tgcccagttc gtgtcgctga cctcgcaggc gccgccggcg
    32581 acggtctgcg tggccaggtc cagccaggcc gcgttgagca ggttggtgct cgcctgcacc
    32641 cggaccctcc gcccgtcggc ccagtccacg ctgaaccgga aagcctccgc cgtgaccgcc
    32701 ggcccgagga gcaggggcgt ccattcctcc gccggagccc cgcagaacgc ggcggaccac
    32761 gaagacgggt tcgcgaggta gtacgccgtg ggctcgctgg cgccggtgaa catgtccgcc
    32821 ccgccgtccg cgcccagggc cgggacgtcg cactggaaat agacgccgtc cagcgcggcg
    32881 cactggccga acacgccgac gcccaggccc gtcacgccgc cgttgaagta gatcgtctgc
    32941 agccccgcgc acgcgtagaa ggcctcgtcg ccgaccgaac ccagcgcggc ggcgatggac
    33001 acggagtcca gcccgtcgca gccgtggaac gcgctgtggc cgacggtctt caggcccgcc
    33061 ggcagggaca gagacgagag gccgaggcag ttgtagaagg ccgcgtaccc gatgttcgtc
    33121 acgcccgcgg ggatggccac cgagccgagc ccccggcact cgtagcacag ctggccgggg
    33181 atggacgtga tgccgtccgg tatgtcgagg gaggcaaggt tgcggcagtt caggaaggcc
    33241 gactcgccga gcgaggtcag cccggccggc agcgtgaccg acgccagggc gcggcacccg
    33301 acgaaggccg agtcgccgac ggaggcgaca ccggccggaa tggtcaccga gaccagtccc
    33361 gtgcagccgt ggaagagcgc cggcgggatc gagccgatcc cggagggcag aacggccagc
    33421 gcgaggctgg aacagccctg gaaggccgat gcgccgagat tcgtcacgct gacgccggag
    33481 gcgaaggcga ggctggtgac gttcgtgcgg tccttgaggg ccgcccggcc gacggccgtg
    33541 accgggtagc cgcccagtgt cgaggggacc gtgatcgcgc ccgtcgcgtt ctccgcgagg
    33601 ccggtgaccg ttgcgcggct gtcctcgacg gcatagtaga aggcgccggt gccggcggtc
    33661 ccctggctca agaccaggtt gtcgaagtag acctgccgga gttcggcgta ctcgtcgttg
    33721 gtgatggccg aagcgtagaa gtggatgttc gcaaggaagc agagccccgt cgcgttgatg
    33781 gtggccgagt aggcctccga cccgctccgg ccggtggcgt acaccgtcac ctgcgtggcg
    33841 ttgacctgtg tgaaggtcca gtgcatggcg gccgtgccgt agttggtcag cgcgttggcc
    33901 ccgttgaccg tgatcgccgc cgacccggcc tggttgaccg tgacgatttc gcggttggcg
    33961 gccgtgcgga gggcgaagcc cttgctgccg ccggcgcccg cgtcgtagtt caagccgagg
    34021 tcgattgaga agacgtcgcc ggtgttcatc gcctggctga aagagcggtc gaggttgatg
    34081 tacgagcccg cgcccttcgc cacgtagccg aacgcgctgc ccatgctcaa tccggcgttc
    34141 gcgctgtccc agatcccgtt ccccgcccac cctccgccgg cgtcgcccga gatgctccac
    34201 gccgcgaagc ccccgccgcc gttggagccg ttcgtccagc cgccgccgta atttgccgcg
    34261 gaatccgcgt tgacgaccgc ccgggccggt ccggccagcg ccagcagcgc cgcgctcact
    34321 ccgaaaatgg tggccttgtt catggtcgcg ctcctagttc accgtggtca ccatggagga
    34381 cgccgtgaag gtcctggtgg tcgtgccgcc cgagactgtt ccgccggtgt acaggccgtg
    34441 gaattccgtg ccgccggcga cggaaacccc cgtgacgatg gtgtaggtcg tgttcgtgat
    34501 cgccggcgtg ctgaagagca gcttcatgcc gccgcccccg ggcccgccgc cgccgcccgt
    34561 gtgggagcgc ggcagccgat acacgatgat gttcgtgctg gccgacttga cctggatcac
    34621 cgtgcccgac gtcgccgttg tggtcgagta caacaccgac cgctgggtgc tgctccccgc
    34681 cgtcggcgtg ctgctcccgc cgcccgtgcc gaccatgacg ccgccgccga tcgcgaaggt
    34741 gtacatgtcg caatcgaacc cctcctcggg ggccgtcgtg ccggaggaaa caatggtccc
    34801 gccggtgtac tgcattggcc cgttgcaatc gatgccgtcg ttgccgaggc tgtagcagta
    34861 gatcctgccg ccgttgatcg cgaggttgct cgcggcgttg atgcaatcgt cgtacgccgt
    34921 gatttcgatg ttcccgccgt tgacggccaa ccgcgccttg ctctcgagcc cctcgccgcc
    34981 gtcgttcctc gtgcttgcgc ggatcgtccc gccgttgacc gtgaggttgc cgcccgcctg
    35041 gatcgccttg ggattggagt agtccgatgc cgacacggcc accttctggc cgcgcgtggc
    35101 cgcggtgatg cacggcgcgt tcgtcgtgcc cgccacgccg atggtcatcg ccccctcgca
    35161 cgagatcgcg tcgcccgcgt tgccctggct caccgccgtg atggtcccgc ccaggatgtc
    35221 caggttccgg tcggccttca ggccgtccgc cgcggcgacg tccttgacgc ccaggtcgtt
    35281 ggtgaaaacg gcggaacaac cgccgctcgt gaggatgtcc aggacgccgc cctggacgac
    35341 gatgctgccg tccgccgaga tgcccttgcc cgccgtcccg acgtgcgaga tggtgatcgt
    35401 ccccgcgttg atcgtgatgt tggtgtcgcc cttgatcgcc gtgcagtagg agggatcgcc
    35461 gtagttgttg gtgatcacca ggtacatgtt gccggtcagg acgaagttca tcgccccgcc
    35521 gttgagggtc atggccccgg tgctcttgaa gcccttggac tgcacgccct tgaccgtcac
    35581 gttcatcgcg ccgccgttga cggtgaacgt gccgtcgcac ttgatgccct tcgtgtcgtc
    35641 ggcgacgctg gtgatggcga tcgcgccgcc gttgatctcg acggtccccg cgtcggcgtc
    35701 gatcccgtcg ctggtcgccg cgatcgtgag cgtgccctgg tccatccgga agtagcggac
    35761 cgcgtggatc ccgtccttga ccgccgaggg gatggccacg tcgccgcccc tcatgcggaa
    35821 gtagccggcg ctgtagatgc cgtgcgactt ccgccccgtg acggtcgcgc ggccacgccc
    35881 gctgacgatc atcgggcccg cgctgtacag cgagccctgg ccgctctccg tgtacacggc
    35941 gctgtcggcc agcgcgttgt ccgtgccgtc cgcgaggacc acgaaacagc ggttggtgga
    36001 ctgcacattg atggccggcc cgttcgtgca ggtcagcgcg acgccgttga gcgtcacctt
    36061 cgaggccggg gcgccctgga tcttcaggta gccccccgtg gccgcgcccg acaggatgta
    36121 ctcgacgccc gcgattgtcg aggtggcggt gatcatggcg ccgttctgcg tgatcgtcac
    36181 gcccgcgccg gcgtggttga ccagcgtcgc cgacgccccg gcgtaggcga tcgtgacggt
    36241 ggtcgtgaag cccgcgttct ccacgtagtc cgcatccgcc gcccaggcgc ccgcgggaag
    36301 aagggccgtc cccgcgagtg cggccaggat gaatttcgcc cgcgtgtgca tcgctttgct
    36361 ccgttttccc gtgatcatac ccggggccgg gttaagaacc ggttaagaaa cggaaaaagc
    36421 ctttccgccg cggcgggcgg cacgcgcaga tgcgggcccc tccggcgtcg gtcagccttt
    36481 tttcaggctc tgcacgaacc gggccatgcg ccgcatggcc tcctggatgt tctccatgga
    36541 ggtcgcgtac gcgcagcgca cgaagccctc gccgcacgcg ccgaacgcgg tgcccggcac
    36601 gacggcgact ttctcgcggt cgaggaactt cagggcgaaa tccttcgacg caatgcccag
    36661 gtgcccgatg tacgggaacg cgtagaaggc gcccgcgggc gtcgtgcacg gcaggcccat
    36721 ctcctcgaag ctcttcacca ggtagttgcg gcggcgccgg tactcggcgg tcatctccac
    36781 gacggccgtc tgcggcttgg tcagggcctc gatggccgcc ttctggctca tggtcggcgc
    36841 gcacagcatc gtgtactggt gcaccttcat catggcctcg gtcagctccg gcggcgcgca
    36901 ggcgtagccc agccggaagc ccgtcatggc ccaggccttc gagaacccgt gcaggaagat
    36961 cgtccgctcc ttcatcccgg gcagcgaggc gatgctcgtg tgctcgccgt cgtaggtcag
    37021 ctcggcgtag atctcgtccg tgatcaccag caggttgcgc tcgcgcgcga acgcggcgat
    37081 ctcgacgagg tcctctttcc ggaggatcgc gcccgtcggg ttgttcggga aattgagcag
    37141 caggaccttc gtccgcggcg tggccttcgc ctccagcatc gcgcgggtca gccggaactg
    37201 gttgtcgcgc ttcgtctcga ccgtgacggg cgtcccgtgc gcgaggatca cgtccgaccg
    37261 gtacgagacg tagcacggct cgtggtagag cacctcgtcg cccgggttca gcgtcgcgcg
    37321 cagggcgagg tcgagggcct cgctgacgcc gacggtgatc aggatctcgg tctccggctt
    37381 gtacgacgcg cggaaggcct tctcgacgta cttcgagatg gcccggcgca gctcgagcag
    37441 gcccatgttg gacgtgtagt gcgtcgcgcc gcgctcgagg gcgaacacgg tgtactcgcg
    37501 gacgtgccag ggcgtgtcga aatccggctc gccgatgccc aggctgatga tgtccttcat
    37561 cgtgctgacg atgtcgaaga aatcgcggat gcccgagcgg ggcatgtcca cgatgtgccc
    37621 ggctatgaaa tcacggggag acttggagtc gttcgtcggc atcctgggtc tccatgagca
    37681 ccccgtggtg cttgtaggtc ttgagcatga aatgggtcgc ggtggacacc acgccgtcca
    37741 gcgtcgccag tttctcgctg acgaagaagg ccacttcctt gaggtccttc ccggccacga
    37801 agagcagcag gtcgtacgcg ccggacatca ggtacaacga ctgcacctcg gggtagcggc
    37861 tgatccgcga ggcgatgcgg tcgaacccgc cctcgcgctc cggcgtgacc ttgacctcga
    37921 tgaccgcggt gacatgggaa atgtcgagct ggtccacgtt caggatggcc tggtagccgc
    37981 ggatgatgcc ctgcttctcg tagtccgcga tccgcgcctt caccgcatcc acggagaggt
    38041 tcagcatccg cgcgatggtc tccggcttct ccagcgcgtt gttcttcaaa atcgtcagta
    38101 attcgtccat aaacgcctcg cttcttaagc cgcacgtgat agccgacgcg ccgccccgcg
    38161 tcgagcgaat tatggggaat gcggccggct caatgatact gccgctgctt ccatgtgata
    38221 ccgcggccgg catagtgccg cgcggcggac cacgtggcgg caaggcagta aaccagcgcg
    38281 gcgggcggga gcagcagccc gcaggcccgc ggcaggcgca ggatctgcag gatcttgcgg
    38341 agcgcgaccg gcatgaccgc gatgagcagc agcccggccg cggccgaagg ccagccaccg
    38401 gccagcgcca gccacgggac ccacgcgccg gcgaacgccg ccgcccacgc gagcagcagt
    38461 cggcccggcc ggtactccat catcgggtag ctcaaccgga ccaggcccgc ccacaggtcg
    38521 cggaacgtgt cgtacatgcg cgtgctcaaa aagtcgtgcg tccagaagag ccgcagcgcg
    38581 aacccgccac gcttgaagtt ctgcgccagg gccatgtctt cgatgaggca gccggcgacg
    38641 gcgcgatgcc cgccggcggc ttcgtaggcg acgcgccgcg cgaggatgaa tgccccggag
    38701 agccgcgagc ccgcgctccc gggccggttg gcccggtcca tcggctcaat cagcgcgctc
    38761 aacgtcgcga acaggggcat gatggttttt tcccagatcg tccggcactc gatcgccggc
    38821 agcacgctta acgcgtccgc gcggccggcc tgcgcggcgg ccatcgcctg cttcagggcg
    38881 gccggatgca gcactacgtc ggcgtccagg aacaggagcc aatcatgctg ggccgccgcc
    38941 gcgccctgga cgagcgccca atttttaccg acccagccgc cgtccggccg gtcggcgccg
    39001 cggatcagcc gaaaggccgc caactcccgg cccgccgcct cggcaatccg gaccgtgtcg
    39061 tcggcggact ggtcatccgc cacgatgact tcgaaggccg ggtagtcctg ccgcccgagc
    39121 gcccgcagcg tggcggcgat gtcgcgctcc tcgttccggg ccgggatgac gaccgacacc
    39181 ggcggccagc cgtcgggcgg cgccggcagg ggcgtcgagg aatccagcgg tgggaatcgc
    39241 gtttcgagat agcggaggcg cgcgaagatc acggcccaga gaagggccga aaccacgagc
    39301 ataaccgcca cgacgatcgc tcccatgcag cggttattta ccacggatta cgcggattgc
    39361 acggatatcc tgtactcccc atccgcgtca tccgtgaaat ccgcgacgga tggagtcatg
    39421 gagggcggag ctctgcgacg ccggagacga tcggaaggtt gtcatccgcg gggtcactgg
    39481 ctattctgcc gacgccatgt ccttggacaa catacgcgtc gtcctggtca gcccgatcta
    39541 cggcgggaac gtcggctcgg tctgccgcgc catgaagaac atggggctga aacacctcgc
    39601 catcgccgcg ccgcgcgggc cgctcgacga cctcgagtcg cgcaagatgg cgctccacgc
    39661 cgacgacatc ctggagaacc gacaggaata tccgacgctg gccgaggccg tttcggactg
    39721 cggcttggtc gccggctgca ccgcgcggcc gggcctctac cgctcccacg ccaaatcgcc
    39781 gcgcgagtgg gccccgcacc tggtccaggc cgcgaagaaa tccaaggtgg ccctggtatt
    39841 cggcaccgag accgacgggc tgtccaacga ggaactcgcc tgctgcaccc aggtcatccg
    39901 tatcccgtcc tcgcccgagt acccctcgct caaccttgcc caggcggtcc tgatctgctg
    39961 ctacgaaatc ttcctggcgt ccgggacctt cgagctgccg ggcgagttct cgcccgaggc
    40021 cagcggcgcg atgcgggagc agatgttcgc catgtggcgc gaggcgctcc tctccgtcgg
    40081 cttcatgaag ggcgacaagg cggaccacat gatgatgggc ctccgccgca tcctctcccg
    40141 cggcaccctg accgtgaagg acatccgcat cctcatcggc atggcccgcc aggtccgttg
    40201 ggcggggagc cagttgtcgc aacctggcga tgcggagaaa taatatcccg gcttcggcgc
    40261 gctgggccag cgcgccctac ctttgcgcac ggatacgcaa tacgccggtc gttggattca
    40321 ggccgaaaat aacgacggcg ttggtgatgg gccggggcgg cgggaaggct gccaaggttt
    40381 cccatgtccc agtagcaaag atactttccg tgaactccag catctgccac gcattcgccc
    40441 cgcctttcca atctacccgc ttcccgctcc agaaatcgcc aaggctcaac agacccagga
    40501 acgagtctcc gttcaacgga tcggtatcgg caatgtattc gtcgagattg accgcgttgt
    40561 ccccgtctgg gtcatctccc gcgtcatcat taaacggatt gagtccgcga tgcgcctccc
    40621 atccgtcggc catcccatcg ttgtcctggt ctgggttcaa gggattcatc cccaagccca
    40681 cttcgtatcc gtcatcccct tcatcgccgt cggtgtccgg attcagagga tcagtctgga
    40741 tcatatattc ctggaaatcg gtcagcccat cgttatctgg attgccggtt ccatcgctga
    40801 ccgcgatgct gccgaagtag ttggcttccc agtaatcaga catattgtcg ccatccgtat
    40861 ccgtaaattc atccgcgccc atatccacac gcgcaccgta tgggcgcgat tcgccatcca
    40921 tgtccgtatc tacaccagcg tagtccggcg aaccggcgtc aatgcagggc gaaccgtgca
    40981 agatatggaa atctcccgcc gctgcattcg caaacatcgt atcggcattg atcagatggt
    41041 tgctggtggt caggctgccg taaatgtcgt cgatgttgca gtaacttgcg tggacttcgg
    41101 attgggcgcc acaatttaga tcagctccgt aggcagaatg attagaaact atcaccgaat
    41161 cgcagatggt cataacgctt gattccccgc cgaccaagat acccccacca ccatctctgt
    41221 ctgggttaac cggggcagaa acgaagtttc tcgaaaatgt acttctttga atagttccgg
    41281 aggagaaagg cgcaatcctg agaccgcctc cagctgtttc acactggttg actgcaatta
    41341 cagaccctgc cactgtaata gatgcgccaa gttcgatttc gacccctccc gaaaggatgg
    41401 aactattccc cgcaagctgt gactgttcaa attgcagatg tgtcccctgt gatgcaccga
    41461 agccaccgcc accaacccgc gccacattgc ttcgaacatc cgtatcgaaa aactcgaccg
    41521 aagaacctag cgaagcatag aatcctcccc cgaaagcatt tgccccattc attgatgcgc
    41581 aactatgaat ggaagactgt attattgagg cgaacgattt tcgtatgtgg actccccctc
    41641 cccactctcc gctgcctctt gtgatggtca ctccactgat ggacagattc gcgccattgt
    41701 ccagcctaat ccctcgacgc tgatactgcc catcaatcat cgtctcatac cattccgtgt
    41761 tctgctccga gaagtcgggg gcataacttc cggttatggt aagatcgtaa tcggcgccgt
    41821 ccagattcag cacggcctcg ggattactgc cggtatatgt tcccatcgcc accagaattt
    41881 cgtcacccgt tgtcttcgcc gcttccgccg ctgcctgcaa cgacttgaac ggcgcttcga
    41941 tggttcccaa ctcctgcgtg gctaaactgt cagcgtaaac gtaaatccgc gaacgcgtgc
    42001 ggaaggtcat gtccgttgag aggcttgtca ggccattggt cccgaatgct tggatctgga
    42061 agtgatactc cgttcctttg accaggttgg tcagatcggc ggtatggttg gttcccattg
    42121 ggtggccgga cagggccgtc atgccgtagt tcgtcgtctc accgtaaaag acacaaccgg
    42181 tcgagggaat gccagtgttc cagtgaaccc aggcgttggt ctcggtgatg ttcatcaccg
    42241 ccacattgag gatttccggc ggatagtcgt agggatttcc gatgcgaacg actaccggct
    42301 tatcgaacca gcccttatca ctggtgattc gcaaggtaaa cattgcatcc gtaaagccgg
    42361 ggaagttggt ggcgatttcg aaggagaacg gcgcggcgga tgtgacggtc attccggcgg
    42421 tcacatccgc gaaagcggcg ttgctcacga tcagggtgat attgataagc gaggtcgaga
    42481 gaaggccggt cacgcccgac aggtccaact catggctggt caggctgacg gccatgtttg
    42541 ccgtttcacc tggttcgata tagccatctc catcgccgac ggcttcggtc accgttgtgg
    42601 tcaatgcgaa cggcaggtcg aaggcttcct gaatacctac ggtggattgc ggctcgggca
    42661 cgaaggtcga gtagccaaga ccacgcttga caaagccgcg ccagagcgca attgaattcg
    42721 agcatccgtt gtccacgaga tccgcttgca ggatggcgtc ccgggcctga tcgaatcggg
    42781 gattgtccgg cgtcagcttg agcccatcca ccaccagttg ttccattaag ccgctgcccg
    42841 tgaacccata ttcctcaatc aggttggctc ggcattccca gagggccagg caccagactt
    42901 cgccaacgtt gtggacctgg tcggcctccg gggcgccgaa gtagggattg caggggattc
    42961 caggcgggaa gccgatttga ttcggatcgg tgtccgcgaa ggtctgcggg gccttggtca
    43021 tgtccgtgga gtacgggaac cgccgaatgc cgaagtacag attggaggtc ggcggggagt
    43081 acatcgcggc gtagtcgccg aagggatagc aggcctccgg cgggtcgctg ggctcgctca
    43141 agaggttcaa cgcgaaaaaa tcgctccagc cttccgccat gccccggggc tggacgatcg
    43201 tcaggccgta cccgttgccg atgattcggg aggtcaagcc gtgtgtcatc tcgtggagga
    43261 ccaggtgtcc gtccaacgcc ccatcacgaa ggggcgaggt ccgggaaaag attgagacgg
    43321 tcagtttgcc ttctgttccg tccccaaaac cggtgtagta ggccttgttc gcatagctga
    43381 agcccaatga agcgccgttc tggacttcga tcttgaaccg gtctccgccc agaccgccgc
    43441 gcccgaagtt gtcctgctgg aagttgcccg caggttcgtc gaagccgaga gcatggagcc
    43501 ggtcatgata gaagttggcc cagtagaagc cctgcacctg ggagaagttt acatagttgg
    43561 tcgggtgctt ggtcaggtcc acggttcggt cgaatacccg gtacgggaag cccgtcaacc
    43621 gggcctggtc gggcaggctg tcatcgttcc agtcggcata gacatccgca ttgttgccca
    43681 gcagctcgtt gctcccgtcg gggatccagc cttccggcga ggcgttggtg tccagggccg
    43741 tcaggagcag catcagccgg gacgcctcga ccggcgtgaa gttcgtcggc tcgtacggcc
    43801 ccggcgtggt cggcatcggg ctgtcgttgg tgaagacgct cagggaaatc gtttctgctg
    43861 gtagggcggc ttggctcaag ccgccgcggc gcgctgggcc agcgcgccct acctctgccc
    43921 acgtcagcaa ttggtcgaag gcaatctttc ccgtatccgc ccggatgacc aggtcatgcg
    43981 cgtcgctgtt gccctcgcac tcgatggcca gctcccagcc caccacgcac gaaatcatat
    44041 ccagcggata ccagaccttc cgcgcgagat aggtcttgcg cacggccccg ccgcccagct
    44101 tttcccgggc gatctccagc gcccgggcct cgtccaccgt ttcggccgga tggaacagca
    44161 ccgacgggaa gtacagcgcc cggctggaca cattgatgat ttcgttgtcc ttggtcagat
    44221 tcagcgtcag gtgcgcgccg aagatgtcca ggccctcgtg ttgctgctgc cagcgcacgc
    44281 gccggatgcc gttgtgccgg gtcgtgtagt cctggaccaa ccgggcgttc ggcggctcca
    44341 ggtcggacgg atgcagggtc atcgtccggt cgttggcccg gatgaacgcc gcgagaatct
    44401 cccgaggcgc ggcccccggc gcgggcggcg acagcaagcc gctacgccgc cggatgagct
    44461 gcggtgtccc gaaatccggg tcgcggttca caacggcatc cggccattga gaaagttgga
    44521 aacccttctc gtccggcatg gcgggctggg cggtgggcgc gaggattgct gaattagaag
    44581 gcacaagcgt cgtgacgctt cgggcaggat catcgcgtgc ggggatccgg gactgcaagg
    44641 ttggagcggt ggccgatatg ccgcgctcat gggtgtgccg ggccttccac acaccccaga
    44701 atgatgcggc caacaccagc atgaccgaaa tcgtcagacg cctcctcata aaagggagag
    44761 tacacaggtc ggtccgtttt gaaaacgaca ccgatccaca atgtcgccta ttccttggtc
    44821 gacaggcccg ccaggtccgt tgggcgggga gccaacttta gcccccgatc gagccagcct
    44881 gatctgcgcc aaaaagccgc tcggcatacg agtgtgtgac aatatgtctc ttgaatgccg
    44941 cgatgcggca tcctttcggt gttgcgagga attattttaa tatgcttatt atcaatatat
    45001 tgcgacgatt accaggcgat ttactgatga cgttggcatg tatgctgctc ttcatccgta
    45061 ggatttttac tggtgtcggc gaaatcgagg tgaaacatgg caaaagtttt gggcattgat
    45121 ctgggtacga cgaactcgtg catggcgatc atggaaggcg gccagccggt ggtcgttccg
    45181 aactccgagg gcggccggac caccccgtcg gtcgtggcgt tcacgaagag cggggagcgg
    45241 ctggtcggcg ccgcggcgaa gcgccaggcc gttacgaacc cgaggcacac ggtgttttcc
    45301 atcaagcgct ttatgggccg gaagtacgag gaagtcgccc atgaaatctc gctggtgccc
    45361 tatgaggtgg tccgcgcggg gaacggcgac gcccacgtga agatcggcga caagacctac
    45421 tcgccgccgg aaatttcgtc gatgatcctc cagaagatga agctggatgc ggaggcctac
    45481 ctgggcgaaa agatcacgca ggcggtcatc acggtcccgg cgtacttcaa cgacagccag
    45541 cggcaggcga cgaaagacgc cggccgcatc gcgggcctgg aagtcctgcg catcatcaac
    45601 gagcccaccg ccgcgtccct ggcctacggc ctggacaaga agaaagacga gaagatcgcg
    45661 gtctacgacc tgggcggcgg cacgttcgac atctcggtgc tggagatcgg cgagggcgtc
    45721 ttcgaggtga aggcgaccaa cggcgacacc cacctgggcg gcgacgattt cgaccagcgg
    45781 gtgatcaact ggatggcgga ttcgttcaag aaggagcagg ggatcgacct ctcgaaggac
    45841 ccgatggccc tgcagcgcct gaaggaggcc gcggagaagg cgaagtgtga gctctccagc
    45901 tcccagcaga cggagattaa cctgccgttc atcacggcag acgccagcgg gccgaagcac
    45961 ctgaccatga gcctgacccg cgcgaagctg gagcagctgg tggacgacct ggtcggccgg
    46021 accgtgaagc ccgtcgaaaa ctgcctgcgg gacgcgaaga tcgaggcggc gaagatcgac
    46081 gaggccatcc tggtcggcgg catgacgcgc atgcccaagg tgcaggacat cgcccagcaa
    46141 ctgttcaaga aagagccgca caagggcgtg aacccggacg aggttgtcgc ggtcggcgcg
    46201 gcgatccagg gcggcgtgct gaagggcgag gtgaaggacg tgctgctgct ggacgtgact
    46261 cccctgaccc tcggcatcga gaccctcggc ggcatcgcga cgacgctgat cgagcgcaac
    46321 acgacgatcc cgacgcgcaa gagcgaaatc ttcagcaccg ccgcagacgg gcagtccacg
    46381 gtggagatcc acgtgctcca gggcgagcgc aagatggcct ccgacaacaa gaccgtgggg
    46441 cgcttccacc tggacggcat cccgccggcc ccgcgcggcg tgccgcagat cgaggtgacc
    46501 ttcgacatcg acgccagcgg catcctgcac gtcggcgcga aggacctcgg cacgggcaag
    46561 gagcagaaga tcacgatcac ggcctccagc ggcttgagca agcaggagat cgagcagatg
    46621 acccgcgacg cctcgtccca cgcggaggac gacgccaagc gccgcgagac ggcggagacc
    46681 cgcaaccgcg cggacgcgac ggtctacgag acggagaagt tcctgaagga gcacggcgcg
    46741 aaggtgtccg ccgacaagaa ggcgagcgtc gagagcgcgg tggccgagct gaaggaatcc
    46801 ctgaagtcgg gcgatgcgga ccgaatgaag gccgacctgg agaaggtcaa cgccgggatg
    46861 caggcgctgt cgggcgagct gtactcgcag gcgcgcgccc agcagccggg cgcgggcccg
    46921 cagggcggcg cgcagcaggc cggcggcccc ggcggggcgg gcccgcaggg cggcgcccag
    46981 ggcgacgtga tcgacgccga cttcgagatg gtggacgaga acaagaagaa gaaataagtg
    47041 ggatgcgccg gcccggcgcc gcccctttac gtagcaacca aacccaaaag ggaggacaga
    47101 gaaggcatga agatacaacc gttgggcgat cggatcctgg tcgagccggt caaggaagag
    47161 gaggtcaaga agggcgggat catcatcccg gacaccgcga aggaaaagcc gacgaagggc
    47221 aaggtcattg ccatcggcac cggcaaggtg gacgacaacg gcaagacgat ccccttccac
    47281 gtcaagaagg gcgacctcgt gctgatgccc cgctacggcg gctcggaggt caagatcgac
    47341 gacaaggaat accagatcct ccgcgaggac gatctgctcg gaatcatcca ggactaaagg
    47401 agaaacccca tggcagcgaa gcaactcgtt tatgatgcgg aagcccgcca gtacatcctg
    47461 cgcggcgtgg agaagttgag ccgggccgtc aaggccacgc tggggccccg cggccgcaac
    47521 gtgatcctcg acaagaaatt cggctcgccg aacgtcacca aggacggcgt gacggtcgcc
    47581 aaggagatcg agctggagca gccctttgaa aacatgggcg cccagatggt gcgcgaggtg
    47641 gccagcaaga ccagcgacgt cgccggcgac ggcacgacga ccgcgacggt cctggccgag
    47701 gcgatctacc gcgaaggcct gaagaacgtc accgcgggcg ccagcccgat ggagctgaag
    47761 cgcggcatcg acaaggccac cgaggtcgtc gtcgagcagc tccagaagat gagcaagaag
    47821 gtcaaggacc gccaggagat ctcccaggtg gcgacgatct ccgccaacgg cgacacgacg
    47881 atcggcacga tcatcgccga cgccatggac aaggtcggca aggacgggac gatcacggtc
    47941 gaggaggcca agagcatcga gaccacgctg gacgtggtcg agggcatgca gttcgacaag
    48001 ggctacctct ccccgtactt cgtcaccaac gccgaggcca tggaagtggc gctggaagat
    48061 ccctacatcc tgatgtacga gaagaaaatc tcgaacctgc aggacctgct gcccgtgctc
    48121 cagaacatcg ccaagtccgg ccgcccgctg ttgatcgtgg cggaggatgt ggacggcgag
    48181 gcgctggcca cgctggtggt caacaagctg cgcggcacgc tccagtgctg cgccgtgaag
    48241 tccccggggt tcggcgaccg gcgcaaggcg atgatggaag acgtcgccgt cctgaccggc
    48301 ggccggttca tctccgagga cctcggcatc aagctcgaga acatccagct ctccgacatg
    48361 ggccgcgcca agcgcgtgac cgtggacaag gagaacacga cgatcgtcga gggcgcgggc
    48421 aagacgtccg acatccaggg ccggatcgcc cagatccgcc ggcagatcga ggagaccacg
    48481 tccgactacg accgcgagaa gctgcaggag cggctggcca agctggccgg cggcgtggcg
    48541 gtcatcaacg tcggcgcgat gaccgagtcc gagatgaagg agaagaagca ccgtgtcgag
    48601 gacgccctgt cggccacccg cgcggcggtc gaggagggca tcgtcgccgg cggcggcgtg
    48661 gcgctgctgc gcgcggccaa ggccatcacc gacctgaagc tcgagggcga ggccgccgtc
    48721 ggccgcgaca tcgtggcccg ggcggtcgag gagccggtgc gcatgatcgc cgagaacgcc
    48781 ggcgtcgagg gctcgctggt ggtcgcgcgc ctgcgcgag
//
LOCUS       DAOOTK010000013        46289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_599_length_46289_cov_5.933880, whole genome shotgun sequence.
ACCESSION   DAOOTK010000013 DAOOTK010000000
VERSION     DAOOTK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 46289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46289
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_599_length_46289_cov_5.933880"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..714
                     /locus_tag="PK634_02950"
     CDS             241..714
                     /locus_tag="PK634_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96725.1"
                     /translation="MNLPTPFFYRIVEREPARVAIVRDILGDWLGGLNRTEGSNSFLL
                     AQLEGKGMNYPWDRRICMPYSGSIVAMTVTLSAGAATNGSATISAAVNGVLNTNLFAT
                     INNTWPTNGAYTAIPPGTLTFSAGDCLGIIGSSTDFDVVPTDRKMIVRLYVTYNP"
     gene            complement(1014..1574)
                     /locus_tag="PK634_02955"
     CDS             complement(1014..1574)
                     /locus_tag="PK634_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96726.1"
                     /translation="MNQILQNSLIRQPRPTFQTSLARGENWSHLPWLVDKLKSFFPDY
                     AGMTVIEHRRNSEWAQAAGIDALITLRSSKTLSIDVKLRSSAYPDVLLEVVSNVERGK
                     PGWIEKDLAMDYLAYGIEPTQTLYLFPFLQLQRAWRMNKADWIRTYGLKTTSTGGWGG
                     KPLYTTQFCSVPVDVLKAAAGETLKA"
     gene            complement(1571..2809)
                     /locus_tag="PK634_02960"
     CDS             complement(1571..2809)
                     /locus_tag="PK634_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96727.1"
                     /translation="MKLSTLEPRDEVEAFVRRHLRDMDSTAYCLYMLALPTLERQQDG
                     YGKPVLASQDSWAQACHFSRPATAKGLRTLAACGLIHLTPGDNGLVTGKRLASKVRRC
                     SIEEIRSLCPREILERFAPPDAERLADVLNRRGVPWHGETISPHWFTTNTGRIESKEA
                     IYRTEGHNTRQERNRAFVASLKPGEVLLNSDYSAAEPSILCHDLQRLGLLTRPVDPAN
                     IYEAILKAASVNRGGAKMLFNPYARRQKTRFISANRDGAKQLFNAIAYSPCRRLVIPQ
                     EWDLPPDHFLRDLIPALERYREHLWKHGRDTPDAPRHVVTLTGRRIEHDRKTRMHRGK
                     PIAWRAQGTVADVLLSTIPAILEAEREGRCRFFFQAHDALFVAVPKDSDFNPAAIMEK
                     AADDAGLPLRVKTTPHEHKP"
     gene            complement(3367..4137)
                     /locus_tag="PK634_02965"
     CDS             complement(3367..4137)
                     /locus_tag="PK634_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96728.1"
                     /translation="MSNPDPETSGLPQRAKRKRRALMTALEIPEQLAIEIHNATKRLN
                     LSEAEKRSFSSDMARVYHYVKNVKKGLGHIVDPLETLSQAKDCLTKIDSALSILEQGC
                     VTESTLLSLALTAGVKPFPCFGGAFSKSSIKAMRAACACELGKLAKLGRTRHRSFQRK
                     IIVDMAANVWKNNLGWQKPPPTGPASPFVSLVQALFSCLGLPGKENATKLIRDVVKRP
                     CFTLYSKEQLLALGYLKPEDCPEYPPEDTTLSPKSQKK"
     gene            complement(4159..5298)
                     /locus_tag="PK634_02970"
     CDS             complement(4159..5298)
                     /locus_tag="PK634_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOW96729.1"
                     /translation="MASIHKLPNCKNWFVQLTNADGKRTFRATHTADRKLARKLADKY
                     DEAARQARARRLTEATARKFIKECYELANLEPLQSDTVRAYAEAWLKRKESELADSSL
                     REYKKTVDHLAASLGKRFERPMDTISRQDAARFRDSLTSRVSGSTTNKYLKIARVLWG
                     SAQRDGLTLDNPFALTPTVRASKGKRRPFTLAELKRILEVCNQEWRGMVLLGLYTGQR
                     IGDLARLTWANTDTVNGEIYFTTGKTGRAMNIPIAAPLMRYFEGLAAPDEPDAPLFPN
                     LVKQPSSTLSKGFRSVLGSAGLAEYSASHDGTGKGRDSKRDNGGLSFHCLRHTATSLL
                     KNAGVGEAVAMEIVGHNSAAVSQRYTHIETSVLREAVNKLPDVTR"
     gene            5394..5474
                     /locus_tag="PK634_02975"
     tRNA            5394..5474
                     /locus_tag="PK634_02975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5454..5456,aa:Ser,seq:cga)
     gene            5857..6669
                     /locus_tag="PK634_02980"
     CDS             5857..6669
                     /locus_tag="PK634_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96730.1"
                     /translation="MLANDIRLRWAILLAVGILCGAAGLLTRTSLKDTGCVPSREKVV
                     SSNTRHDRLDFNVVKGLKVSDADKGWGIRVGSVDIRRKPIGFIRLGAFNEARITDLRV
                     TMSAQQDPSSFATLLHRLSSSPDTLETVLRRGERDRARLAYLLDQFGTRSPYRNTRIS
                     SVHIEGLSLQVSPSNAESDIMLLEADSVDFHGRRCRLNGAVSFGSLDGQRVRCREAEL
                     TLGTDPVISTHPAILQAAGATQEVETLSFPLAALLTGEDLRPYMNQSPTRTR"
     gene            complement(6645..12254)
                     /locus_tag="PK634_02985"
     CDS             complement(6645..12254)
                     /locus_tag="PK634_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96731.1"
                     /translation="MKKRAMMGLVLFAALATDVRCATPPGPVRSGPDAETMLEWNGVC
                     LPFVENVGQVRRPEVRYIANIFGGTILVSEQGWVSLALIGRADADGQREGCVLRESFP
                     SRAAAPVRGMGRIAAPVSYWKGKDLSRRIHDAPAYAAVDLGAVAPGVTLTLRAFGRNV
                     EKLFVVGPDAKPADILCKLEGAKRLSVTGAGRLAAETEGGVVEFTRPVAYQETGGART
                     AVEVAYVVRGLEYGFAVGAYDSSRPLVIDPLLASTFLGGSGDDSMAGLVIHTNGSVFA
                     VGTTVSPDFPSTPGAFETAGPGDEDVFISKFSSGLSNLLASTFLGGAGIDHARAVALG
                     TDGTVYVTGYTSSDDFPAATNAFQDTLFADSAFIARFDPALTNLMAATYLGGNFDDDG
                     RAIRIATNGYVYVAGVTHSASFPTTTNAFALTYPGWGSWSQYTLSVRLKRMQGLTSFR
                     LCESDLGYYEFAFGPGDELDVSKVDHIEWLVTELAATQAVGLVTGVWQDVSISASNGL
                     LRVSLNGTPILNVQDTTPHILTGGDIWLKSYVDTWGRGTTSEAYVDNVLVSNQQGVVW
                     FDDFEQDNLDNLWAFPLPVSDVGPAEWIRTEFEGQFCLHGLTTLTNGDWGYAEASPKE
                     PGSEYAGFVARLNPELTELKAATFLGGEEANYIEDLALGSGGRVHVAGTTYSPDFPTS
                     TNAFDRTFDGDWMNYGVSLRFKLDSGAAELNVRHSEGEDARAGRYRILFRQDHVEVAK
                     ERPKGMLRLLGQANPTLATGTWHQVLAASTSSGLVVRVDGALVLTVSDRDPLVLGGVS
                     LGVGSGSVAAVSFDDLAITNAAGAIYTDDFESGDIAAWTASEGWAVLDDGGNHVLRGI
                     PGGTNVWARADTPWFLYDTEGRCDGFVVTLDGALSTLLGSTFLGGAGQDAATSVAVGS
                     NGTVYAGGWTLSSDYPTSENAYDRGDYSEPGFITRLDQNLTNAQASTLLGEGATIEAL
                     AVARDGSVFVAGDVSGYMPTSWDAYDESWNGFPDVFASRLSGDLTTLLASTLLGGTNE
                     NHAFALALAPDDSVYVAGSTRSPDFPVTNACDAAWGGGEDGFISRLDAFLSRAPRPYI
                     SVQPASRQFGYVLVGVAVTQVFDVVNTGRVPLAIGTVSLTDGLDLVHFKLTNDHCSGQ
                     SVDPLSTETCRVSVIFRPTNVWGKAVHLVIPSDDPDTPQRKVPLYGSGRSTNAAEALF
                     LEGLQALTNYLGDSGLTSDLLTANDKFGQALAQNPNHYAAVWYRLITRVAALPYDSEV
                     AQLLTDLGMPEAGRDLLNWTAELPDPLPPDSPDVGYAVGVLDNRMVNIIEEGLLNLAR
                     IPTNWTGNILLSPEYLPIDNEVQVDAGDAQVVQGILSCGRGLLWMIQSHDWHMSFADL
                     LNDDLTLGDHLETYPMLGTLTNVPRFARAAALFIQAIDYYQTGSSLIRAETDGQDDDL
                     LIFDPDQLNEEAVFRHALEQIRDSLSGTNASPFRFELSQELTLKYMFTNPISPRVLAT
                     GLGAQQIMASNILFQVDRGLANMATLTESFSETLSPDHSPVKRNVEVDYGDIALIRAC
                     LQLCRALILVAQAYDMDDVNLVTEYERNPLLVGDIPAAHTRFLGITNTAALAAASNAL
                     SAALAGYQVASAFIRSEADDQADDLFVIQSNRLWLAEIRRFGHEEDSFRTLVQSLSNS
                     LSGPVLVDYVDHQGAHEFYETMHLGRVFTTNYVTRAHLPEFDATNRPVSGTFPDPTFN
                     GLFPEMSQWHVADALDLGLVDSDGLGLPDLWQLHYFGTLGHNPATVAASGMTLYDEFH
                     ARTDPGNAASCFMLTRGAFQAGDPACVQLRWRSRPYQRYVVEWATNIPYGSWTEEAIL
                     PATPYTNFYTNRVEDTKRKYYRVRVGD"
     gene            12698..14293
                     /locus_tag="PK634_02990"
     CDS             12698..14293
                     /locus_tag="PK634_02990"
                     /inference="COORDINATES: protein motif:HMM:NF038132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038132 family protein"
                     /protein_id="HOW96732.1"
                     /translation="MARIPFVLVVSALLCCSGLDAQAINILGWSSEGTCGTSESNGVV
                     TASPFSSQYGWVSTASGVPDMGLPGIGGSGGPSSGSRIRSPVFRGKAGQALTFFFNYV
                     TSDGSGYSDYAWARLLDTNLNEVALLFTARTTQGGDTIPGFSMPSPAATLIPASTPII
                     AGGPLWSPLGESSGACFGNGCGHTGWIQALYTLPAGGRYILEIGVVNWNDSSYQSGIA
                     FDGVLLDGQDIQEPTPVTLTAVTAQRVEGGVRVSWETAMEFENLGFHVYRSASPDGER
                     TRVNAELIAGTGTSDGAAYSIVDTEAPESGTVFYWLEDVSTSFATEMHEEVALFDDIS
                     DKADEEPPALAKFELGPESSVCRIGYAALKAAGAPVDTTDPATLQVFIDGEETALFVS
                     AWRGPMEDGDYLLFYVPEGTETRSCEIRAGANALRMEEVFAGPNDNGDAVWYGLADET
                     GALAFEATMEVSRYLLVGFADTFLCVLDVTDALHPKILYGYAWFFHEGSEGLYLSYLT
                     EAPAQMMAAGGGAIREIESLSQP"
     gene            complement(14324..15274)
                     /locus_tag="PK634_02995"
     CDS             complement(14324..15274)
                     /locus_tag="PK634_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96733.1"
                     /translation="MDQTLRSPAFQMVIPEGWTFEGGLVWDQRNIAFPAQLIYRVVSP
                     DGQTALEQMPALGFIWAQGGLMGGFGVQYGREMRMPMDAPQVLTEIVIPRYRGNRQPG
                     IVSPPEPAPDYLQAIVTGSGAQDLPPQMRGGRVRIQYDQFIEEIACITAYSQTNGGSL
                     WACAAIIGIRARTDMFPALCPMLRGMIASIQGNPGWHAAVNQVQSSLIQGQMANIEAA
                     GQLSRSISAQNNAMIAQMNARRAATPRMDGGGGGGGAYRFGQAMRGTEDAVDSHDGHT
                     VEIPASGTNAWSNALGDRMVTDDPNFNPNLHSNQDWREMK"
     gene            complement(15377..15451)
                     /locus_tag="PK634_03000"
                     /pseudo
     CDS             complement(15377..15451)
                     /locus_tag="PK634_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            15908..17437
                     /locus_tag="PK634_03005"
     CDS             15908..17437
                     /locus_tag="PK634_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW96734.1"
                     /translation="MELYHNQVTRLQGPASLRLHHRSFGLYFIAANVDADVTVLDFPS
                     RRRFIREIRKGYDIVGLSFITPNYAKAKEMARLVRLHAPGSVIVLGGHGAAIEGVEDL
                     IDCDHVARGEGIRWMRTFLGQEPAAPIVHPVLPCSEYESIFGVPIPGPTPSVLVPGLG
                     CVNACNFCCTSHFFEKAYVPFLRTGREVFEQCCRIAGARRTPVFSVMDENFLKDHARA
                     LDLLAEMEKNRRWFSFYISSSAEAIAAFGIENMVRLGVTMVWIGFESQSADEFAKNAG
                     LDAARLVRDLRRHGVSVLGSAMLCMEHHTPENIGADIDFVVGLNADMIQFMLYTGMPV
                     TRLYEDHKRRGLLKDDLPYEEWHGQNQLNWRHPGFPGGEPKRWLDAAFRRDFEVNSSS
                     IYRMTETAVRGYRTLAAMTSRDACLEARMQQLRRRARLYSHLLPVIERRAVNEKERRR
                     AIALDAEQREILGPIRITERLTRLGARLLAARWDVRLRLLGDRLQPATIVTRYRGGRV
                     R"
     gene            complement(17497..17799)
                     /locus_tag="PK634_03010"
     CDS             complement(17497..17799)
                     /locus_tag="PK634_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOW96735.1"
                     /translation="MATKLYVGNLSFNTTEGDLQRLFEQAGRVVSCTIIMDKFTNKSR
                     GFGFVEMSSQEEANKAIADFNGKDMDGRALTVNEAKPREERPRGGGFGGGGRREWR"
     gene            complement(18106..21231)
                     /locus_tag="PK634_03015"
     CDS             complement(18106..21231)
                     /locus_tag="PK634_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96736.1"
                     /translation="MRLGKWRKVAVCAWCVLSVSACAADWYVKTNGSDGAAGTNWATA
                     KATIQAAVDAANPGDVVWVTNGVYDAGGTTNRIIDSVLTNRVAIYKPLTVRSVNGPAA
                     TFIVGRPASGLGGEGIDAVRCVSLTNGAFLGGFTLTNGHTTGSVSGSDGCGGGVWGAY
                     SNAVVSNCVITGCTAWEGGGAYAGRLEDCRIVGNEAGQGGGVSYSYGPPAKLLRCALF
                     RNSTYNWGGGAYNAELTGCEVVSNFASSGGGGAMYSTLSNCVVNENYGHYGGAAFSCT
                     LANCLMISNSAGYGGGAYRSTLNNCTVAGNIVSDDGGGVLSCTLYNCIVYYNQAPEGP
                     NWYVESYGFATSIYFSCTTPLPPKIGNFTDAPGFVDAAGGNYRLASNSPCVNTGLYQN
                     WMASARDLDGSNRIAHAVVDLGAYEYSGPLTLPVTAATVAGPAVTLTNRFVTNTVSVV
                     PPGATPPLYYSWSPAPTSGQGTAQAVYRYSTPGTKVVTVLVSNLLSGAVGTGIVHIAV
                     ASNAYVAVTGNDANDGFRWATAKRTIQAGVDAVSTGYTVWVSNGVYDTGGRPCGGQTL
                     TNRVSLDKFVIVRSVNGPEHTVIVGQADPFNPDGEYGPAAVRGAYLTNRAVLAGFTVS
                     GGRTDHQEDSPFTAMEYGGGLYCVSTQSVVSNCWVMNNVAYQDGGGVMGGTLQSCRIE
                     SNRAASGGGVAGDMVSGGAVVLSNCVLAGNSTWNWGGGAYNARMHGCRVSGNQAASGG
                     GGAAQSVLYGCVLESNTANSGGGVEGGAAFSCVMQSNAAVWGGAAERSLLVNCTLIGN
                     AASQSGGGAANCVLSNCIAWFNSAPEGTNWYVDEWETTNIAYTCAVPLPPGPGNIAAD
                     PALRDAAAGDWRLATHSPCVDKGTNQAWMTGALDVEGKPRMLNARVDMGACELVPPSW
                     DSNTNGLPDWWEWDYSHSFTGMSATADGDGDRVLNRDEYGARTDPTNAESYLGMALPP
                     LAGGPTGLVVRWRSVKDRTYHVDRATNLLSSSAFTNVFANVPGQADYTSVTDATATAA
                     GPYLYRVRTQP"
     gene            complement(21344..21583)
                     /locus_tag="PK634_03020"
     CDS             complement(21344..21583)
                     /locus_tag="PK634_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HOW96737.1"
                     /translation="MKVKIDRAEDALYLRLDGAPVIESERIAPGVIVDLDRRARVVGV
                     EVLQLSRRVRFAGPRRADLAAPSLVREKPAKKYRA"
     gene            complement(21580..21831)
                     /locus_tag="PK634_03025"
     CDS             complement(21580..21831)
                     /locus_tag="PK634_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOW96738.1"
                     /translation="MRYRLSRHAAHVLHERGIAEEWLERVLREPQKVEPDEEDRALEH
                     RLACIPEHGHRVLRVVVNKSVRPEKVVTVYFDRAMKGKL"
     gene            complement(21828..25040)
                     /locus_tag="PK634_03030"
     CDS             complement(21828..25040)
                     /locus_tag="PK634_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOW96739.1"
                     /translation="MKAAFESFQDELNRLVAAFEQGFQAFTAPDYSEARVREDFLNPL
                     FRALGWDLANRRGLVQSQREVEIESRTDVAGRAKRADYLFRADGRERFVCEAKKPRET
                     LGERYAFQAKRYAWNKGLPLAILTDFEDLKVYIVGGRPRPDRPDDGLWKTWNFRQFSL
                     CAREIWDLLSRERVAAGSIEALLDSLPKARPVKGKARQPWLIRPERTKALDQDFLEYL
                     DEQRLSLARDILANNTREDMLAEGRLNEAVQHILDRLLFLRICEDRDIDTGLRLAKIV
                     ETWRRAYGHEDRRRPKQGHFFREASAAYRAAPPAESLWAAVVGHLQALDRRPPGNMPH
                     FNGNLFKPHFSEDLRVGDQWLCDFIEDLSDDESPYLFNVIPVEILGSVYERFLGKVIQ
                     PRGRGVAADEKPEVRKAGGVYYTPRYIVDYIVEQTVGKQLDEIAGVGRVGSTRRRDGG
                     LGQAALPESPTYAVFDKKTRALKILDPACGSGSFLLRAFERVGEHYQRRFTEHPEDRK
                     PDRCWTDPATGDVHLAVDLKRQILRNNIFGVDLDAQAVEVTQLSLYLKMLEGEDRQTI
                     KQQRELFRDDTALLPPLENNIKHGNSLIASDFSLVPEDLMRVHAFDWDIQFAEIMKGG
                     GFDAVIGNPPYVRMEEFAPIRKYLRSHYKAHEERADVYIYFLEKTLSLIGNTGLIAMI
                     VSNKFTVAKYGQPLRSTIKNMGCVYEIADFAGARVFKGATVRTIVLVMGRNEKSPSSR
                     TRYIPVPSAASFESIESGRLSVAQYANDAEQFLSPGQLTERPWALIPAGRADLLAKLS
                     ERFPDLVEAFGWKPLFGIKTGLNEAFVLDADARQHLLKTDRKNHEVIKPFLFGKDVKR
                     YAIDDSGRHVIYFHNGGHPKEFPAVKTHLERFKSALNQRAAHQEWYELQQPAVALLPL
                     LAKPKIVYPIIAPESRFALDDKGYLVNDKLFVLPTDSRFLLGLLNSKLGNFFFTSVCA
                     RLEGPGACYFEFRSQFVERFPVAGIRHSSPTDRARHDKLVELVDKMLVLVPKLRAEAS
                     ESKRKTLQNAVDATDRQIDRLVYELYGLTGEEIALVEGGA"
     gene            complement(25071..27626)
                     /locus_tag="PK634_03035"
     CDS             complement(25071..27626)
                     /locus_tag="PK634_03035"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96740.1"
                     /translation="MLGTREELERWIAARRGELAGGGIGLDGGEPGRMEPGLFDAARV
                     RVLIARLSSYRDVLHSITHRMLYWAARNADGAFVDLAFWPPPRDAALMARDGVPWWFG
                     TASRQPPARFDVVAISLSVQQEALNLPAAMRRSGLKLSWAARAADARHPLVLLGGNAA
                     GAAPFLHGDAEGKDSGGLVDAVCFGDGLTWLPRVLSAWAEARATGMDRPEFLARLARD
                     LPGTYVPAFYRHEYSGERRAAIRPLRDEPPARVTYRKDPAETWMNGYDGAFIPFSDED
                     TEETLPLAAGCAYRCRFCQTGWMRGSLDVADPAGFLGAARRLRAAMAASDLNLLASDA
                     CSIGRLAALIPPLQEIFPRLSVKSLSLFSLAHRGDLRALVQGLDKREFTFGVEGVSAR
                     LRAWLGKPVSDAELEAVVRELAPAGLRRIKLFFIATGREESADWAELTRLLGAVRAAA
                     PGAALTASFTPLFHAPFTPLQFAEIIPLSGPMIREAEAAARAGGAEFRWSAWPDEIQW
                     TNLLCRLGRAATPLLVKLSLDSGLRYDEHLPPAALQETLAWLRDSGRDVAGRMAECRE
                     EDILPWDDFEGGTPRERLWRQFLRASNEPTEERAVPVSGAGPRPSPRSRKSARPVVFL
                     AWLPPPDAARPGVTLARALLRDFFAARPDAVRWYAGSPELIRPFPGHGLAFVRASFFE
                     GWTPGPAEIRDADGAARRQLLAGPLPGGAAPDPDECVFAVRFAGDARAFAAWMRRRGV
                     PFQDFRNERGLWSVVPASRRHRSGLLYLLNGGSEPSFLAAGLRLSAFLGRDGAALLRG
                     AELIGVYGPPSGHCPACGAAARRALVRTAAPLELCEGPCAGKAGSTSRGRAVT"
     gene            27804..29006
                     /locus_tag="PK634_03040"
     CDS             27804..29006
                     /locus_tag="PK634_03040"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOW96741.1"
                     /translation="MKILIVDKFPDRYIAAIRTLGHDVAYEPGTRATDIAAKSGDADI
                     IVVRSTEVTAETIQASKNLSLIIRAGAGTNNIDRKAASAAGVYVSNCPGTNSVAVAEL
                     AMGLICALDRRLVENVSDLRAGRWNKAEYSKAAGLLGKTLGVIGVGMIGKELIARARA
                     FGLKVKAWSRSLTPEKAEALGVEYAASIAALVPTCDIISVHLAQAAETKGIISREVIA
                     TMKPGALFINTARAGVVDQAALVEAVKAKRIRVGTDVFEGEPEGKDGPFADELGMLPG
                     VYGTHHIGASTEQAQDAVATETVRIIKVYGETGRVENWVNKMKKTPARFQLVVRHYDK
                     PGVLADVLAAIREANVNAEEISNVIFDGAQAACCTIQLDSALDAGRLAKINALSGRVI
                     HAAQVALA"
     gene            complement(29119..29382)
                     /locus_tag="PK634_03045"
     CDS             complement(29119..29382)
                     /locus_tag="PK634_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96742.1"
                     /translation="MSSGANVHFALEEIKKAKGRKLRVVTLLGDHMNRHLTEERCVTR
                     GRTEPLNARPRSHGRALFLRGPSGRLLFDLTPLRRFTIRCIHI"
     gene            complement(29477..30610)
                     /locus_tag="PK634_03050"
     CDS             complement(29477..30610)
                     /locus_tag="PK634_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96743.1"
                     /translation="MNRIRSVLAKEVIQIRRDPRILGLLIVAPVLQLLVLGSAVTTDV
                     REIRLAVRDNDRSYHSREAVRALSSSGYFLTTMLDGPEVRDADRLVHGDAGLVLVIPA
                     DFSRQLLRGRPARVQALVDGSDSNFGVLGLNYLQKALRQYSGGLVRADAADAARRGRA
                     SAPGITAESRAWYNPSLDSRRYMVPAVMGLLLMVATMVITSMALVKEREEGTMEQVIV
                     TPLRPSELIAGKLLPFVGIGFAEVTLAVAVIVFLFRMPLKGSLVLLYAGSGLFLLNTL
                     GLGLLVSTLVKTQQQAMLVATFFIMLPFVLLSGFTFPVENMPAPIRAVAECIPLKFYL
                     RIVRGLFLKGVGLRRLWPDALALLGSGAAILGLAVLNFRKRLD"
     gene            complement(30607..31728)
                     /locus_tag="PK634_03055"
     CDS             complement(30607..31728)
                     /locus_tag="PK634_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW96744.1"
                     /translation="MKRFWAIFKKEFRQIGRDPLSLGLLIFIPALLLVLYGYALSFDV
                     KHIRVAAADYDRTPESRDFLDGLFENPYFDRAGALGSSTEADAVLDRGRARAVLVIPR
                     GYAADLARGGAADVQALVDGADATTAATTIGYLDALAERAALEAQADLRATPAARPAV
                     IAVPRVWFNPELESAKFLVPGLIALLLMLSAVVATSLSIVRERERETMEQMMVSPLRP
                     EELVLGKTLPYVLICLATMAMVLLLGYVLFGVAIRGSFLLLGLATLLFLFAALGMGVL
                     ISAVTRSQQVAFQVAVIASLLPSIILSGFIFPIRNMPLPIQGVTLILAPRYFVSALRK
                     IILKGAPFQAVWMDLLAMLLLGVAFNLLAAHKTRKALWS"
     gene            complement(31725..32678)
                     /locus_tag="PK634_03060"
     CDS             complement(31725..32678)
                     /locus_tag="PK634_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96745.1"
                     /translation="MNREGKEPVIAVDGLTKVFGRFTAVDRLRFNVERGEIFGFLGPN
                     GAGKSTTIRMLCGLLSSTAGTATVGGYDINRDPEAVRQNIGYMSQKFSLYKDLTVAEN
                     IAFFGGVYGLARPRLRERAGAVVRLAGLDGLEDRLAGTLSGAHQQRLALGCAILHEPP
                     ILFLDEPTSGVDPISRRHFWDLIQDLAGRGVTILVTTHFMDEAEFCGRIGFISGGRLV
                     ALDTPAAIKRDAVREDLYEVSLADMQRARERVEAVPGVVATSYFGRHLHVFGRPGELT
                     AEGLGRALAEGGMTVNSVERVPVSLEDAFIRLAATGREAAA"
     gene            complement(32691..34007)
                     /locus_tag="PK634_03065"
     CDS             complement(32691..34007)
                     /locus_tag="PK634_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW96746.1"
                     /translation="MRAKCALLFLALGPAGFAATEPPEPVCADAIVAQALAYSLRLKS
                     AEQDVRAAAASLAQAGAQGLPTVSAEARAAHYEGLKETALSPTMIIPEIPDRYGAVIG
                     VSQPLYTGGRVSSLKQAAALQSDAARQALAGTESDVVLEALSSYWSWSRAFFALRALR
                     ASVDRMEAHARDMKNMNEAGLATDNDRLSTEVLLERTRLQRTEAERNEALARAKLDFL
                     TGASLPSNAAADEAAASPAADPAPESVLLEMAATNRPEVRAAALEARSAAKAVRAARA
                     GYGPELALSARYEQARPNTLIFPPEDEWQDDAFVGVVASWALFDSGLTRGRVLEARAR
                     AEQADLRRRQAEDRVALEVRQARVALANAGERAVVAGRARDSAALNLQSATDLWQNGL
                     ARHADVLDAHAQLTDAEFALVSARADAVVARAALDHAVGILAAPKP"
     gene            complement(34004..34942)
                     /locus_tag="PK634_03070"
     CDS             complement(34004..34942)
                     /locus_tag="PK634_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW96747.1"
                     /translation="METGNSADAIETHGLARSFGETRAVAGLDVLVRRGEMFGLVGPD
                     GAGKSTAIRLLCGILKPDAGEARVLGFDLLREAGPIKSRIGYLSQNFTLYGDLTIDEN
                     IEFFADLHGVRGFRARRDELLDFTRLTPFRRRLAQALSGGMKKKLALACTLIHRPELI
                     LLDEPSTGVDPVSRGEFWNILSGILAQGVTILMTTPYLDEAERCGRIGLMHGGRLFMT
                     GTPAEIRAAMPGRVYEVACPAPASAYRVLRRRWSSANVVLYADRLRLSFREGASALAE
                     AKGLLETEGCGPVRASEAAPSLEDAFVGLLGEGSST"
     gene            complement(34945..35907)
                     /locus_tag="PK634_03075"
     CDS             complement(34945..35907)
                     /locus_tag="PK634_03075"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW96748.1"
                     /translation="MKRIRARVTMVAAGIAVLAGCGMRDGQPDGSGTIECTQVQVSPQ
                     VAGRIVELPGQEGDAVAQGALVAKLDPVDYELKRDEARAALAQAQAQLDLMLAGARDE
                     DIQRAREQVREAGAAAKAAEADRVRIRQVFDKKSATQKQMDDAQAAADRTAAALAAAQ
                     QNLDKLVRGNRQEEIRAAQAAADLARARLAQAEKAVADCVIIAPIAGVITTKVREAGE
                     YVGPGAALLTLSRLDEVWLSVYIPETRLGRVKLGQPARVRIDGDARVFEGKVTFVSPE
                     AEFTPKNVQTPDERAKLVYRVKITLPNPDGVFKPGMPADGYLEK"
     gene            complement(35904..36554)
                     /locus_tag="PK634_03080"
     CDS             complement(35904..36554)
                     /locus_tag="PK634_03080"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOW96749.1"
                     /translation="MARRDSPHGARILEAAIRVFAERGLKGATIRGVGRAAGVNSALL
                     YYYFADKNALFVEAVSRVLERFLAHLKERRRTFRGAAERLRYLVDGIFDYYTRHPERM
                     DLMWLAVSRHPEEFGRALRRLVAGHELVPLEVLAEGMRRGELRRMHPVEAWWSILAMC
                     LFSLKVQTVVRHMGPVPIRRPSAGPAERKEQILGLLLHGVVEKRGGRSPAGRNRGA"
     gene            complement(36765..37187)
                     /locus_tag="PK634_03085"
     CDS             complement(36765..37187)
                     /locus_tag="PK634_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96750.1"
                     /translation="MTARPKSRLKREEMAALAKTAVEKKLRLSSIIAEKEVAYLSAIA
                     DSHRDAGPPSGQSNLSFFAAVIPWVSSFIQVYAKQTDYVAALAWASNQPKRNEKPVSL
                     LTTLMTTYFIERGYERSLSQSKEDKDTWAAIRHRLDYL"
     gene            complement(37184..38098)
                     /locus_tag="PK634_03090"
     CDS             complement(37184..38098)
                     /locus_tag="PK634_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96751.1"
                     /translation="METPQVQGQRVEVDNGRLTVSFSLGAAPGPAEKDRSKWTFAGAA
                     NSVRYHFYEHRLEMLDLFNHHMTRFNPDGHEPEKRCMQIQRLTLFDSRLGTDTPVDVD
                     VFNLEYIRRDEKPEPPSNSDQVVFIDCPLDGNLLGINGLSRCRLRRTLTWKNMANYVL
                     EHMDVVMDTPSGVVDAIEFQAQFLACMDFGLAPQKKPNKDSLEEGWFTCQWLHDSPFA
                     LRFAYGFACNTGLQSFENPVPNYPTPGISQKTRFKTYSWIAGKTKSLDVAHLFTYGTT
                     TDSDGTLIRRLRTELPPSGGCGLAGGRK"
     gene            complement(38161..39831)
                     /locus_tag="PK634_03095"
     CDS             complement(38161..39831)
                     /locus_tag="PK634_03095"
                     /inference="COORDINATES: protein motif:HMM:NF040095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96752.1"
                     /translation="MWRNPDLSLSGTRNLLNPLAGYWGRAAMPQGRTRVSRIHGDLNL
                     GNLLIGLTPNHAPANAFIIDLANCRPAAPTAIDFARLETEFWHEILPTLLTEGKMAED
                     LVTARGMLDFGEGDIKSLTPLVRNAVLFVNAIRREALKILGADQDEYVLGDYMHALYF
                     LHGRALAFPSVQQFVGKCRVALTGAAMALDFLHRLEAGEVRLPPVLPTSAGGLSNAVR
                     ISRPVRRSTRPEPNAGPRREVGRRSADIGSANEDISKAKVRARANPSVQPTASVEIGN
                     GRLTAKFNLNPQLDHTEAARFGSYFAGAATSVKLDDHNMLDIFHQLVVLSGSYAPPDL
                     RTRIMQIRRIRPLGFRPVDLFGQKHVLIGRKDVGPERSIVIQCRFQATGTRRPHARRV
                     LFLNRRFTVKQGDYHFQEDISLTDRSPSVEGAKRLTFYLDFFGYIDMSRSPAKRTDMD
                     GWFTIANVNDNTFAPRFSYGFASSAPLAKDMETSNHCARAALGGERFAWKTYSWSTNR
                     CDHQIVAHRFGYGATTDAEGKEISQLWQHVLKTREPTRETISPIAISP"
     gene            complement(39794..40450)
                     /locus_tag="PK634_03100"
     CDS             complement(39794..40450)
                     /locus_tag="PK634_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96753.1"
                     /translation="MNADTLVRKNNSKLMTKELDSSRREILHAALVDMKLLGRSAVLP
                     NPYSRRSGVLERSAVWFLVIPRPNGAELQIVAKFDEPDRAKKEWQRIEELRGLNTPPQ
                     ALLPVAKNRKQHGVVVYQNANIPTPSGEVSTLQELLQCQLATNVNNCLKALDLCFDAL
                     RPFYLSEPGALRFIAGGRHLCWKDWLPKKAACRTAMLKGARQALPGLVEESRSLSLRH
                     "
     gene            40508..41446
                     /locus_tag="PK634_03105"
     CDS             40508..41446
                     /locus_tag="PK634_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOW96754.1"
                     /translation="MSENYPTPLLDLEERFASETACREYLERLRWPNGFICPQCGVAR
                     AGRMQRGLWHCRGCHRQTSVLAGTVLQDSHLPVRIWFRSMWHITSQKNGVSALGLQRA
                     LGLGSYRTAWLILHKLRRAMVRPGRERLHGVVEVDEAYWGAGEEGVIGRQTETKALVG
                     VAVEVKGTGMGRIRLATLPDLLGTTLQRFLDHAVEPGSTVRTDGLPAYGGMKGYRHER
                     QIQNRQPAGDHLLPHAHRVISLLKRWLVGTHQGAVSHEHLDDYLAEFTFRFNRRMSAS
                     RGKLFYRLVQQVAQVSPQPYEMVTNPQPIVPDESSV"
     gene            complement(41435..41614)
                     /locus_tag="PK634_03110"
     CDS             complement(41435..41614)
                     /locus_tag="PK634_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96755.1"
                     /translation="MKLLMIISPEARQGEIRELIGRHDVRAYTELTRVIGEGATGKHL
                     GTHAWPGQSVIGLYT"
     gene            complement(41686..44868)
                     /locus_tag="PK634_03115"
     CDS             complement(41686..44868)
                     /locus_tag="PK634_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOW96756.1"
                     /translation="MEQRQGFFSSVIQAFLKGNLAVLLILISLAAGAVALLVTPREEE
                     PQIVVPLADVMVMYPGGSAEEVEQLVASRLEKLLYQIDGVEYVYSMSRPGMAVVTVRF
                     FVGEDREESLIKIYNKVFQNIDKTTPGIAGWVIKPIEIDDVPIVTAALYGPGLDAGAL
                     HRVAEEVSGKLQRIPDSARITIHGGRKRVVHVYLDTERLTAYGLSPMEIAGALKISNT
                     QIESGSFAKADREIAVEVGPFLQNVDEVRNLMVGLHHDRPVYLRDVAEVVDGADEVAS
                     YSRIGFGPGSGPTDSESRPAVTIAVAKKKGSNAVMVAREVEKALAGLRGSVIPDEVQV
                     RITRNYGETANEKVNDLVMNLVMGLITVVGMIALAMSWREGVIVGLAIPITYSLTLLF
                     NYLLGYTINRVTLFALILALGLLVDNPIVGVENISRHLALRRFPRLQAVALAMEEVMP
                     PIVLATLSIIASFVPMFFITGMMGPYMAPMALNVPLTMISSLFVALAITPWVSNKLLK
                     EPSADTAAFDVKATATFRVYDRLVRPFLDSRGKGNLLLLVMGGLFVFSIVLAATGLVP
                     LKMLPFDNKNELQLVVDLPEGATLEATDAVVRDLEDYLRTVPEVVDFTATGGEGSPMD
                     FNGLVRHYYLRQGPNVADVRINLLPRQKREMASHGIALRIRKDVEALAARHGALVKIV
                     EAPPGPPVLSTVVAEIYGGPHHDYDELIAAGKTVGARLREEQGVVDVDDTVEADQQKL
                     FFRVDREKAGLNGISTEDVVNSLRLAMSGFPVGTVHVPTEQNELPIVLRLPREKRSDP
                     ARLLTLPVKGRMGNSVQLGEIGRSEENVYDKTIFHKNMRRVVYVTAEMAGRGPAYAVL
                     AMQAWLKKNPLPSGIEASWTGEGEWKITLDVFRDLGLAFSAALLAIYVLLVYETGSYL
                     LPVVIMLSIPLTLIGIMPGFWLLNVLTGRTIGGYADPVFFTATAMIGMIALGGIVQRN
                     AIILIDFIRSATERGQDLKEAIIEAGAVRFRPIFLTAGTTMLGAWPITFDPIFSGLAW
                     SIIFGLFVSTAFTLVVIPVVYAMIYGKKGV"
     gene            complement(44872..45918)
                     /locus_tag="PK634_03120"
     CDS             complement(44872..45918)
                     /locus_tag="PK634_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW96757.1"
                     /translation="MSKLASLIKSAWRPVAGVVGLAVVIVWSAGSCAEKVPPGVVEAP
                     AGIAVPEGAATVEVKAEPLATRIHVVGTTASDEKINLSARIPAYVKAVFARAGDVVTN
                     GQVLVTLDDREIREQLAAAEAQLRRAESEYERARGLFEKNATTQQALTAAESMFQSAR
                     AQVDQVKVMLTYAQITAPIHGIVTDRRIEAGDLANPGMPLLAVYDPARMRLEAPVPVR
                     LIDRLALGQEVDVALERPARTVKGRVAEIVSEVDPSSRTQKVKVRLEGVAGEVLPGTF
                     GRLWVEEEAREAIRIPKSAVYRSGQLEFAQVVRDGRAIRRLVKTGPEYGDDVEILSGL
                     KPGESVLAEPRREE"
     gene            complement(45893..>46289)
                     /locus_tag="PK634_03125"
     CDS             complement(45893..>46289)
                     /locus_tag="PK634_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW96758.1"
                     /translation="RSRGAVAAARAELDAARAEEAKAERNLRLDLTQAYLGAKEAHER
                     MEVTRKSLESAREALRITREQYEQGAADVALLLQAQVGVTAMRTRAVAAEYDTSPRGR
                     TSTGRWGSWWGGISMDQRRTEHEQARFAD"
BASE COUNT         8433 a        15012 c        14495 g         8349 t
ORIGIN      
        1 tagttacttg taggacccgt tttttgcggt tgaccttaag ggcttaatgg tggttgacct
       61 taagggctta atggttgttg ggccgacggg tttgaaattg ctggcgttga cgagagcgga
      121 atactggaat tccaaggggc agcggtcagc aactactact cgttggaatt tgcaaccacc
      181 cttgacgggc cttggactaa ctggggggct gtatctgacc agccgataac aggggcagta
      241 atgaatctgc cgactccgtt tttctaccgg atagtggagc gggaaccggc aagggtggcg
      301 atagtgcggg atatccttgg agactggctt ggcggtctaa acaggaccga aggatcgaac
      361 tcatttcttt tggcccagct cgaaggaaag ggcatgaatt acccatggga caggcgaatt
      421 tgcatgcctt attctggttc aattgttgcc atgacggtca cactttcagc aggagcggca
      481 actaatggct cggcaacgat aagcgccgca gtcaatggcg tccttaatac aaacctcttt
      541 gccactatca ataatacatg gcctacaaat ggtgcttata ccgcgattcc tccaggcacg
      601 cttacattct cggcgggcga ttgcctcgga attattggaa gctcgacaga cttcgacgtg
      661 gtccccacag accgcaaaat gatcgtccgg ctctacgtca cctacaatcc ataactcttc
      721 cccggtgagc gtcggccaag aacgactccc gcgaatacag aaggcggccc cgcaagcagc
      781 gcgcaacctg cgggagttaa cggattagtt ttccgtgagg aatcgtcaaa tataggtcaa
      841 ctaatatatt gcggcatgag agcggggagc ggactcacgg aaaaacgggg gggggcggcg
      901 gctttctata gggtaaagat ttttacaact ggaccggccc agaaacagac accagtggta
      961 aagattattg ccctatacca gcggccgagc cttcgccttg ggcttgcctg cttctatgcc
     1021 ttcagggtct ctcctgctgc cgctttcagc acgtccacgg ggacggaaca gaattgcgtc
     1081 gtgtaaagag gcttcccccc ccaccctccc gtgctggttg tcttcagccc gtaggttcta
     1141 atccagtcgg ccttattcat acgccaagcc ctttgaagtt gaaggaaggg gaacaggtat
     1201 agggtttggg ttggttctat gccgtaggcc agataatcca ttgcaaggtc tttctctatc
     1261 cagccgggct tgcccctctc gacattactg acgacttcca gaagaacgtc gggataggcg
     1321 ctgcttcgca gtttcacgtc aatggaaagg gtcttggaag aacgaagcgt tatcaatgcg
     1381 tcaatcccgg cggcctgtgc ccattcagag ttgcgccggt gttcaatgac ggtcattccg
     1441 gcataatcag ggaagaagga tttcagcttg tccacaagcc acggcaggtg tgaccagttt
     1501 tcgcccctag ccaagctggt ctgaaacgtt ggccggggct gtcggatcaa tgagttttga
     1561 agaatctgat tcatggcttg tgctcgtggg gggttgtctt gacgcgcagg ggcaagcccg
     1621 cgtcgtcggc ggccttctcc atgatcgcgg cggggttgaa gtcggaatct ttcggcacgg
     1681 caacaaaaag ggcgtcatgc gcctgaaaga agaatcggca acggccttcc ctctcggctt
     1741 cgagaatggc cgggattgta gacagcaaaa cgtcggcaac agttccttgc gcccgccatg
     1801 caatcggttt gccccggtgc attctggttt tccggtcgtg ttcgattcgt cggcccgtca
     1861 gcgtgacaac gtggcggggg gcgtcgggag tgtcccttcc atgcttccac aagtgttctc
     1921 gataccgttc aagggccgga atcaggtcgc gcaagaaatg gtcgggcggc aaatcccatt
     1981 cttggggaat cacaaggcgg cggcagggtg aataggcaat tgcgttgaac aactgctttg
     2041 caccatcccg gtttgcagaa atgaaccggg ttttttgtcg gcgggcatac gggttaaaca
     2101 acatttttgc cccgccccgg tttacgctgg cggcctttag aatcgcttca taaatgttgg
     2161 ccgggtccac ggggcgggtc agcaagccaa gcctttgaag gtcgtgacat agaattgagg
     2221 gttcagcggc agaataatcg gagttcaaca atacttcgcc cggtttcaga ctggctacaa
     2281 atgcgcggtt gcgttcctgc cgcgtgttgt ggccttccgt tctgtagata gcttctttcg
     2341 actcaatgcg cccggtgttc gtcgtgaacc aatgcgggga aatggtttca ccgtgccacg
     2401 gcacgccacg ccggttcaga acgtccgcaa gtcgttcggc gtcagggggg gcaaaccgtt
     2461 ccaggatttc acgcggacaa agtgaacgga tttcttcaat ggaacaccgg cgaacctttg
     2521 aagcaagtcg tttgcccgtt accaggccgt tgtcgccggg ggtcaggtga atcaggccgc
     2581 aagcggccag agttcgcaag cccttggccg tcgcggggcg cgaaaaatga caggcttgcg
     2641 cccatgaatc ttgactggcc aggacgggct tgccgtagcc gtcttgttgc ctttcaagcg
     2701 tgggcagggc cagcatatac aagcaatacg ctgttgagtc catgtctctt aaatgccgcc
     2761 ggacaaaggc ttcaacttcg tcgcggggct caagcgtgga cagtttcaac ggcttcaccc
     2821 tgggcccggt tgggcgttag tgactggagg tcaagtttcg agagatacgg ctttgcgttt
     2881 ggcaatcgtc ggcacgtccg gcaactcgcg cacacgttcg ccgggccgcc gcgtagtggc
     2941 aggatcatgc cgggcgtttc cgttccgcac ctgtggcaga aggccgggcg gggtcggtag
     3001 tgttcatacg acgtgcccat gaacacgccg ggcggcaggg gcgggcgggc gctcacgatt
     3061 cgccaccggt cgagtgcttt tcaagaaacg cccatagcga aggcaagtag actgtgcgca
     3121 ctccgcgaag acagccgggc cgcttcaggc aggcactttt tattaaacca cgcctgatca
     3181 gcccgtaaat aaacgccctt gaaaggcccg tattcggctc ctgtccacga gcaggaatcc
     3241 gaatccaacg ctgcaaaccg cccccaatcg tctccatgtt tcccttcccg tcgtggtggt
     3301 tagatacacc acaactatct aactacaatc gtactgtcgg gaaccgttgg ggcctattgc
     3361 cctaagtcac ttcttttgag acttagggct tagggtagtg tcttcgggcg ggtattcggg
     3421 acaatcctcg ggcttcagat agcccagagc aagcagttgc tctttcgaat agagcgtaaa
     3481 acaggggcgc ttcactacgt cccgaataag ttttgtagcg ttttcttttc cgggaaggcc
     3541 aaggcaagaa aacaaggcct gaacaagtga aacaaacggg gacgcggggc cggtcggcgg
     3601 cggtttctgc cacccaagat tatttttcca gacattcgcc gccatatcca cgattatctt
     3661 ccgttgaaag cttcggtgcc gtgtgcgccc aagcttagcc aatttcccaa gttcgcaggc
     3721 acatgcggca cgcatggctt taatactcga ttttgaaaat gctcccccga aacacggaaa
     3781 tggctttaca ccagcggtga gggcaagact caaaagagtt gattcagtca cgcagccttg
     3841 ctccaaaatg gaaagcgcac tgtctatttt cgtcaaacaa tccttggctt gagaaagcgt
     3901 ttccagcggg tcaactatat gccccaagcc tttcttgacg tttttcacgt agtggtaaac
     3961 tctcgccata tcacttgaaa aagaacgttt ctcggcttct gacaaattaa gcctcttcgt
     4021 agcattatga atttcgattg caagttgctc gggaatttca agggcagtca ttagcgcccg
     4081 gcgtttgcgt ttcgcgcgct ggggtaggcc gctggtctct ggatcaggat tgctcatgtt
     4141 ggtgtgcttt cgagtttatt agcgggtcac gtccggcagc ttgttgacgg cttcgcgcaa
     4201 tacgcttgtt tcaatatggg tgtaacgctg tgaaaccgcc gcgctgttat gcccgacaat
     4261 ttccattgca acggcttccc cgacaccggc gtttttcaac aggctggttg ctgtatgccg
     4321 caggcagtga aaacttaacc cgccattatc gcgcttggaa tcccgcccct tgcccgtccc
     4381 gtcatggctt gcgctgtact cggcaaggcc agcagaaccc aagacgcttc tgaagccctt
     4441 tgaaagcgtg ctggaaggtt gcttgaccaa gttcgggaaa agcggggcgt cgggttcgtc
     4501 gggggcggcc agcccttcaa aataacgcat aagcggggca gcaatcggaa tgttcatggc
     4561 tcggcctgtc ttgcccgtgg taaaatatat ctctccgttc accgtgtccg tgttggccca
     4621 tgtcagccgg gccaagtctc caatgcgttg cccggtgtag aggccaagca acaccatgcc
     4681 ccgccattcc tgattacaga cttccagaat ccgcttcagt tcggccagcg tgaacgggcg
     4741 acgcttgccc ttgctagccc ggacggtcgg ggttaacgca aaggggttgt ccaaggtcag
     4801 gccgtcccgt tgagccgaac cccaaagcac gcgggcgatc ttcagatact tgttggtcgt
     4861 ggagccggat accctgctcg tcagagaatc ccgaaagcgg gccgcgtctt gccgggagat
     4921 cgtatccatt ggccgttcaa accgcttgcc caggcttgcg gccaaatggt caaccgtctt
     4981 cttgtattcc cgcaggcttg aatcggccag ttccgattcc ttgcgcttca gccatgcttc
     5041 ggcataggcg cgaaccgtat cggattgcag gggttcgagg ttggccaact catagcattc
     5101 cttgataaac ttacgggccg tcgcttcagt caagcgccgg gcacgggcct gccgggctgc
     5161 ttcgtcgtat ttgtcggcca gctttcgggc caacttccgg tcagccgtgt gggttgccct
     5221 gaacgtccgc ttcccgtccg cgttggtgag ttgaacaaac cagttcttac aattcggcag
     5281 cttgtgaatc gaagccatga cgcttttttc ctttccgttg ggtgtccaca tacaccttta
     5341 tgccattctc tttatatcat accttatatc gttcagggct gtcaatagcg gttgtggacg
     5401 tattattatc taatgatttc cagcatatca gggattgacg ggtgttgcgg gttcgagtcc
     5461 cgtcgtcccc gccaaatctt tattcagcac ccccagagat gggggtgctt tattttcgcc
     5521 ctgatttgac gggcattcgc tacagcctgt cgaatttcca gcaaaagggt gccggaaatc
     5581 tgtcaaaacg ggcttgcggc aggaggggcc tattccactg gcctattcgg ggatgggact
     5641 gacagggact tcagcaacgg cgacccttcc cggtatctgc gtcaatcctc tgcctgtatc
     5701 tcgggagcag cctgtgccag gtcgagtttg atccagggcg ggaggcacaa ggggcgaaag
     5761 ctttttacca gccctgatca acgccacaca gcagcaacta cactgcacac tttattcctg
     5821 caggctgatt cgcgatacta tcatatggag cgctaaatgc tcgcgaatga catcaggctg
     5881 cggtgggcga ttcttctggc tgtcgggatc ctctgtggtg cagccggcct gttgacgaga
     5941 acctccttga aagacacggg atgcgttccc agtcgtgaaa aagtcgtttc gtcgaatact
     6001 cgccatgacc gacttgattt caatgtggtt aaagggctga aggttagtga tgcggacaag
     6061 ggttggggca ttcgagtcgg atccgtggat attcgccgca agccgatagg atttatccgg
     6121 ctcggagctt tcaatgaagc ccggatcacg gacctgcggg tcaccatgtc ggcacaacag
     6181 gatccgtcca gttttgcaac tcttctgcac cgactcagct cgtcaccgga cacgctagag
     6241 accgtgttgc gacgaggcga acgcgaccgg gcaaggctcg cttacctcct cgaccagttt
     6301 ggcaccagat cgccctatcg caacaccagg atttccagcg tccacataga gggcttgtcg
     6361 ctgcaggtat cgccaagtaa tgcggagtcg gacatcatgc tgctggaagc cgactcggtt
     6421 gacttccacg ggaggcgctg tcggctgaac ggtgccgttt ctttcggcag ccttgacgga
     6481 caacgcgtca ggtgccggga agcggagctg accctgggca ccgatccagt gatatcaacc
     6541 catccggcca ttctgcaggc agccggagcc acgcaggaag tcgagacctt gtcttttccc
     6601 ctcgctgccc tgctgaccgg cgaggacctg cggccttaca tgaatcaatc gccaacacgg
     6661 acccggtagt atttgcgctt cgtgtcctct accctattcg tgtagaaatt cgtgtagggc
     6721 gtggccggca ggatcgcttc ctccgtccag ctgccgtacg ggatgttggt ggcccactcc
     6781 accacatagc gttggtaggg acgactgcgc catcgcaact ggacgcacgc gggatccccg
     6841 gcctgaaaag cccctcgggt cagcatgaag catgacgcag cgttgcccgg gtcggtgcgc
     6901 gcatggaatt catcgtacaa ggtcatgccg gaagcggcga ccgtcgccgg attatgcccc
     6961 agtgttccga agtagtgaag ctgccacagg tcgggcaggc ccagtccgtc tgaatccacc
     7021 aatcccagat ccagcgcatc agctacatgc cactgcgaca tctccgggaa caagccattg
     7081 aatgttggat ccgggaacgt gcctgatacg ggcctgttgg tggcgtcgaa ttccggcaga
     7141 tgggctcgcg tcacatagtt cgtcgtaaag acacggccca ggtgcattgt ttcgtagaac
     7201 tcatgagccc cctgatggtc gacataatcc accaggaccg gaccggacag gctgttggac
     7261 aggctctgca ccagcgtccg gaaggagtcc tcctcgtggc caaaccgccg tatctctgcc
     7321 agccatagcc ggttactctg gatgacgaac aggtcgtcgg cctggtcatc cgcttccgat
     7381 cttatgaagg ccgatgcgac ctggtaaccg gccagagcgg cgctcaaggc attcgaggcc
     7441 gcggccagag ccgctgtgtt cgtaatgccc aggaaccgag tgtgcgcggc cggaatgtcc
     7501 cccaccagga gcgggtttcg ctcgtactcc gtgaccaggt tgacgtcatc catgtcatac
     7561 gcttgcgcga caagaataag cgcccggcag agctggagac acgcccggat cagagcgata
     7621 tccccatagt ccacctccac gtttcgcttt accggcgagt ggtccgggga aagcgtctcg
     7681 ctgaacgatt cagtcagcgt cgccatgttc gccagacctc ggtcgacctg gaacaggatg
     7741 ttggaggcca tgatctgctg ggcccccaac ccggtagcca gcacgcgtgg gctgatgggg
     7801 ttggtaaaca tatacttaag ggtcaactcc tgggagagtt caaaccggaa cggcgaagcg
     7861 ttcgtcccgc tcaatgagtc cctaatctgt tcgagcgcat gccggaagac ggcctcttcg
     7921 ttcagttggt ccggatcaaa gatcaggaga tcatcatcct gtccgtccgt ttctgcccgg
     7981 atcagagaag acccggtctg ataataatcg atggcctgga tgaacagggc ggcggcgcgg
     8041 gcaaaccgcg gaacattggt gagcgtcccc agcatcgggt acgtttccag atggtcaccc
     8101 agcgtgaggt cgtcgttgag caggtcggcg aaggacatat gccaatcgtg cgactggatc
     8161 atccaaagca ggccccgccc gcaggacagg atcccttgga ctacctgggc atcgcctgcg
     8221 tccacctgga cctcgttatc aatgggcagg tattcggggc ttaagaggat gttccctgtc
     8281 cagttcgtgg ggatacgcgc caagttgagc aggccttcct cgataatatt caccatcctg
     8341 ttgtcgagca cgccgaccgc gtatccgaca tccggggaat ccggcggcag gggatcgggc
     8401 agttcagccg tccaattcaa gaggtcccgc cccgcctccg gcatgcccag gtccgtcagc
     8461 aactgggcca cctcgctgtc atagggcagc gcggccaccc gagtgatcag tcgataccat
     8521 accgccgcat agtggttcgg attctgcgcc agcgcctgtc cgaacttgtc gttagcggtc
     8581 agcaaatcgc tggtcaggcc cgagtcgccc aggtagttcg tcagcgcctg aaggccctcc
     8641 aggaacagcg cctccgccgc gttggtgctc cgtccgctac cgtacaaggg caccttgcgc
     8701 tgcggcgtat ccgggtcgtc gctcgggata accaggtgga ccgccttgcc ccagacgttg
     8761 gtgggccgga agatcaccga cacccggcag gtctccgtgc taagcgggtc cacggactga
     8821 cccgaacaat gatcgttggt aagtttgaag tgcaccaagt cgaggccgtc ggtgaggctg
     8881 accgttccga tcgccagcgg cacgcggccg gtgttgacca cgtcgaacac ctgggtgacg
     8941 gctacgccga ccaggacgta gccgaactgg cgcgaagcag gctggacact gatatacggg
     9001 cgcggagccc gggaaagaaa ggcatccaat cgggatatga agccgtcttc gcctccgccc
     9061 caggcggcat cgcaggcgtt cgtcacgggg aaatcgggcg accgcgtcga gccggcgaca
     9121 tacacgctgt cgtccggggc cagcgccagc gcaaaagcat gattctcgtt agtaccgccc
     9181 agcagcgtgg aagcgagcag ggtcgtcaag tcacccgaca gcctggaggc aaagacatcg
     9241 gggaagccgt tccaactttc atcataggca tcccacgacg tgggcatata cccgctgaca
     9301 tcgccggcga caaagacaga accgtcacgg gcgacggcca gcgcttcgat ggtcgcgcct
     9361 tctcccagga gcgtcgaggc ctgcgcgttg gtcaggtttt gatccagccg tgtgatgaat
     9421 ccaggctcgc tatagtctcc tctgtcgtag gcattttcgc tggtcggata atccgaagat
     9481 aaggtccagc ctcccgcgta caccgtcccg ttgctcccaa ccgctacgct cgtggccgcg
     9541 tcttgtcccg ccccccccag gaaggtactg cccagcaggg tcgaaagtgc gccgtccagc
     9601 gtgacgacaa aaccgtcaca gcggccttcc gtgtcgtata aaaaccaggg cgtatcggca
     9661 cgcgcccaga cattcgtccc gcccgggatt ccccgcaaca cgtgattgcc gccgtcatcc
     9721 aggaccgccc aaccttccga cgcggtccat gccgcaatgt cgccgctttc gaagtcgtcg
     9781 gtgtagatcg cgccagccgc gttcgtgatc gcgaggtcgt caaaagagac ggcggcgacg
     9841 gaaccggaac ccactccaag cgaaacgcca cccagcacca gcggatcgcg atccgagacc
     9901 gtcaagacca gggccccgtc cacccggaca acgagtccgc tcgaggtcga ggccgcaaga
     9961 acctggtgcc aggttcccgt ggccagggtc ggattcgcct gacccagcag gcgcaacatg
    10021 cccttggggc gctccttggc gacctcgacg tgatcctgcc ggaacaggat gcggtatcgc
    10081 cccgctcgcg cgtcctcccc ctccgagtgg cgcacattca actccgcggc gccggaatcc
    10141 agcttgaaac ggagcgagac cccgtagttc atccaatcgc cgtcaaacgt gcggtcgaag
    10201 gcattggtgc tcgttgggaa atccggggaa taggtcgtgc ccgccacatg gacgcgccca
    10261 ccggaaccga gggccaagtc ctcgatgtaa ttggcttctt ctccacctag aaacgtcgcg
    10321 gccttcagct cggtaagttc aggattcaac cgggctacaa agcctgcata ctcggagccc
    10381 ggctccttgg gggaagcctc ggcataaccc caatcgccat tcgtcaacgt cgtcaagcca
    10441 tgcaggcaga actggccctc gaactcggtc cgaatccact cggcagggcc tacatcggaa
    10501 acggggagcg gaaaggccca gaggttatcc aggttatcct gctcaaagtc gtcaaaccag
    10561 acaacccctt gctggttgga aaccagcacg ttatccacgt aggcctcgga ggtcgttccg
    10621 cgtccccagg tatctacata ggacttaagc caaatatcgc cgccggtgag gatgtgaggg
    10681 gtggtgtctt gtacgttcag gatgggtgtt ccattcaggc tcacgcgaag cagcccgttg
    10741 ctggcgctga tactaacgtc ctgccacacc cccgtaacca gtccaacagc ctgcgtcgcg
    10801 gctaattcgg tcacaagcca ttcgatatga tccaccttgg aaacgtccaa ttcatcgccg
    10861 ggaccgaacg cgaattcgta gtaacccagg tcgctttcac acaggcgaaa actcgtgagc
    10921 ccctgcattc tcttgaggcg gacggacagc gtgtactggc tccacgatcc ccatccggga
    10981 tacgtcagcg cgaaggcgtt tgtggtggta gggaaggacg ccgagtgcgt cactccagcc
    11041 acgtaaacat atccatttgt ggcgatccgg atggcacggc cgtcatcatc aaagtttccc
    11101 ccaaggtacg tagccgccat caggttggtc agcgccggat cgaatcgggc aatgaaggct
    11161 gaatcagcaa acaaggtatc ctgaaaggca ttcgtggccg cggggaaatc atccgaagac
    11221 gtatagcccg tgacatacac ggtgccatcc gtgcccaggg ccacggcgcg cgcgtggtca
    11281 atcccggcgc cgcccaggaa cgtcgaggcg agcaggttgc tcaagcccga tgaaaacttg
    11341 ctgatgaaca catcctcgtc gcccggcccc gccgtctcga aggcgcccgg ggtcgagggg
    11401 aaatccggcg aaacggtcgt gccgacggca aagacgctcc cgttcgtatg aatcacaagc
    11461 cccgccatgc tgtcgtcgcc gcttccaccg agaaacgtgg aggccagcag gggatcaatg
    11521 accaggggcc tggaggagtc ataggcgccc acggcaaagc cgtactcaag gccgcgcaca
    11581 acgtaggcga cttctactgc ggtccgggcg ccgccggttt cctggtacgc taccggccgg
    11641 gtgaattcca cgacgccgcc ctcggtttcg gccgccagcc gaccggcacc ggtcacggac
    11701 aggcgcttcg ccccttccag tttgcacagg atatccgccg gcttcgcgtc cggcccgacg
    11761 acaaacagct tctcgacgtt acggccgaac gccctcaatg tcagcgtgac gccgggcgcg
    11821 accgccccca ggtcgacagc cgcataggcg ggcgcgtcgt gaatccgccg cgagagatct
    11881 tttcccttcc agtacgatac cggggccgcg atacgcccca taccgcgcac cggtgcggcc
    11941 gcccgcgagg gaaaggactc ccgcaaaacg cacccctccc gttgcccgtc agcatccgcg
    12001 cgcccgatca gggcgagact gacccaaccc tgctcgctga ccaggatcgt tccgccaaag
    12061 atgttcgcga tgtatcggac ctcgggccgg cggacctggc cgacattttc gacaaaggga
    12121 aggcaaaccc cgttccattc cagcattgtt tcggcgtcag ggcctgaacg aacaggcccg
    12181 ggaggggtgg cgcatcgcac atcggttgca agggccgcaa acagcacaag acccatcatg
    12241 gcacgcttct tcacaatgac ctcctggccg gaagaaattc aatgcacgga ccacgcccat
    12301 aagccggcac gcccactgtg ttcaatcaac cccctgcatg ctaacgccaa aaacgcccgg
    12361 ggcaaatatt tattaatctt ttagtgccag cgaataacaa atcaagagaa ttatgaatga
    12421 acaggactgg taaagccgcg aggccattga gggggggcgg gtgctgacaa caatgacggc
    12481 tgccgctcga aacaatatcg cctgcgccaa atcggtcgcc gcgcaagtcc gcgggattct
    12541 ggcagacgaa gaacgggccc tatcacgggc gacccggctc ttccggcgtc aggcgccgtt
    12601 ttaagaaaat agatggcgtg atatttttaa gtcagtacac tgctgtccaa ggtatccggc
    12661 ataaaggcaa ctctggccaa gcaaaggaga tcggaccatg gcgagaatac cgttcgtgct
    12721 agttgtttct gcattgctct gctgcagcgg actggacgcg caggccatca acatccttgg
    12781 ttggagcagc gaaggaacat gcggcacatc cgaatccaac ggagtagtga cggcatcgcc
    12841 attcagcagt cagtatgggt gggtctccac cgcctccgga gtgcccgaca tgggattgcc
    12901 gggcatcgga ggttcaggcg gcccttcaag cggttcgcgc attcgctccc ccgtttttcg
    12961 cggcaaggca ggccaggcct tgaccttctt ttttaactac gttacctccg atggatccgg
    13021 ctattcggat tacgcatggg cgaggctgtt ggacactaat ctgaacgaag tggcacttct
    13081 attcacggct cgaaccacgc agggcggaga taccatcccc ggcttcagca tgccatcgcc
    13141 ggcggccacc ctcatacccg ccagcactcc gatcattgcc ggcggacccc tatggagccc
    13201 cctgggcgaa agttcgggcg cctgcttcgg caacgggtgc ggacataccg gctggataca
    13261 ggcgctttat acacttcccg ccggcgggcg ctatatcctg gagatcggtg tcgtgaattg
    13321 gaatgactcg tcctaccaga gcggcatcgc cttcgacggc gtgctgttgg acgggcagga
    13381 tatccaggaa ccgacgcctg tcacgctgac cgccgtgacg gcgcagcggg tggaaggcgg
    13441 cgtgcgcgtc tcgtgggaaa cggccatgga attcgagaac ctgggcttcc acgtctatcg
    13501 ctcggcctcg cccgatggcg agcgcacccg ggtgaacgcg gagttgatcg cgggcacggg
    13561 cacatccgac ggggctgcgt attccatcgt ggacacggaa gccccggaaa gcgggacggt
    13621 cttctactgg ctggaggacg tgagcacgtc cttcgcaaca gaaatgcacg aggaggtcgc
    13681 gctattcgat gatatcagcg acaaggccga tgaggagccg ccggcactgg cgaagttcga
    13741 acttgggccg gaaagcagcg tgtgccgcat cggatatgcc gcgttgaagg ccgccggcgc
    13801 gccggttgat acaaccgatc cggcgaccct ccaggtgttc atcgacggcg aggaaaccgc
    13861 cctgtttgtc tcggcgtggc gcggccccat ggaagatgga gattatcttc ttttctatgt
    13921 gccggaaggc acggagacaa ggagttgcga gatccgcgcg ggcgcaaacg ccctgcgcat
    13981 ggaggaggtc ttcgcggggc ccaatgataa tggcgacgcc gtgtggtacg gcctggctga
    14041 cgagaccggc gcgttggctt tcgaggcaac catggaagtg tcccgatacc tcctggtggg
    14101 gtttgccgac acgttcctgt gcgtgctcga tgtcaccgat gcccttcacc ccaagatact
    14161 gtatggctat gcctggttct ttcatgaagg gagcgagggg ctgtacttga gctacctcac
    14221 agaggcgccg gcgcagatga tggccgccgg cggaggggcc atacgggaga tcgaatccct
    14281 gagtcagccc taggcctaag caccggtacg ggagtcgccg cggctacttc atctcccgcc
    14341 aatcctggtt ggagtgcagg ttgggattaa agttggggtc gtcggtcacc atccggtcgc
    14401 cgagggcgtt cgaccaggca ttggtgccgg acgcgggaat ctcgacggtg tgcccgtcgt
    14461 gcgaatccac ggcgtcttcc gtcccgcgca tggcttggcc aaaccggtag gccccgccgc
    14521 ccccgccacc gccatccatc cgcggggttg cggctcgccg ggcattcatc tgcgcaatca
    14581 tggcattgtt ctgggcgcta atggagcggc tcaactggcc cgccgcctca atgtttgcca
    14641 tctgcccctg gatcagcgaa ctctggacct gattcaccgc ggcgtgccaa ccgggattgc
    14701 cctggatgga cgcgatcatg ccgcggagca tcgggcatag ggctgggaac atatccgtgc
    14761 gagcccggat tccaatgata gccgcgcagg cccagaggct ccctccgttg gtctgggagt
    14821 aggccgtaat gcacgctatt tcctcgatga actggtcgta ctggatgcgc acgcggccgc
    14881 ctcgcatctg tggcggcagg tcctgcgcac ccgatccggt cactatggct tgcaagtagt
    14941 ccggtgctgg ttcgggaggc gagacgatcc cgggctgccg gttgccccgg taacgaggaa
    15001 tgacgatctc tgtcagcacc tgcggcgcat ccatgggcat tcgcatttcc cgcccgtact
    15061 gcaccccgaa cccgcccatc aagccccctt gtgcccagat aaaccccagc gcaggcattt
    15121 gctcaagcgc tgtctggccg tccggagaaa cgacccggta gatcagttgc gcgggaaatg
    15181 cgatgttcct ctgatcccac accaacccac cctcgaaggt ccatccctcc ggaatgacca
    15241 tctggaacgc cggcgagcga agagtttggt ccatgcagac aacgcctctg aatcgcatgc
    15301 cctgcggcgg ggccgccggg ttcggcatcg gcggcgcgac ggggctttct ggcggattcg
    15361 gcgcccggcc gagcggtgcg ccgcaatgcg gacaaaacgc ggccccgatg ggaggaatca
    15421 tttggccaca ctgcgagcac gttacgctca tgttctgcgc ctcccgcgcc attcgttcca
    15481 tgacgccccc gacggcagat atcataacct gacgatgcct ctcgtgaagc tttttaatgc
    15541 tcgggactga tcacgccgcc atcgctcacg atcgcaacac aagtcgggca ggattggacg
    15601 gcgggccgtt tcgaccggtt cattggggga ttgccagacg gtgacgtgga tgatggattt
    15661 gtgcatgggg acatccggtc aggcgccgcc ttccggaccc gcgagcggga tttccttgtg
    15721 cccccgccct gcacgcttca tcctgcgcgc aggatagccg atagaacacc gcatggcgca
    15781 gcacccacag gacatccccg caggcgggcc ccgatggcga gggccgggac gggctccacg
    15841 cctgcttctc tgcggcgttt ttggaccgta cggcattgac gacctgtacg ggcgccggga
    15901 aaacaccatg gagctgtacc acaaccaggt aacccgcctc cagggcccgg cctccctgcg
    15961 cctccatcac cgctcgttcg gcctctactt catcgccgcc aacgtcgacg cggacgtgac
    16021 ggtcctcgac ttcccttcgc gccggcgctt cattcgcgag atccggaagg gctacgacat
    16081 cgtcggcctc tcgttcatca cccccaatta cgccaaggcc aaggagatgg cgcgcctggt
    16141 ccggctccac gcccccggct ccgtcatcgt cctcggcggg cacggcgccg cgatcgaggg
    16201 cgtcgaggac ctgatcgact gcgaccacgt ggcccgcggc gagggcatcc gctggatgcg
    16261 gacgttcctc ggccaggaac ccgcggcgcc gatcgtccac ccggtcctgc cgtgcagcga
    16321 atacgagagc atcttcggcg tccccatccc gggcccgacg cccagcgtcc tcgtccccgg
    16381 cctgggctgc gtcaacgcgt gcaacttctg ctgcaccagc cattttttcg agaaggccta
    16441 cgtcccgttc ctccgcacgg gacgggaggt gttcgagcag tgctgccgga tcgccggggc
    16501 gcggcgcacc cccgtgttct ccgtgatgga cgagaacttc ctgaaggacc atgcccgcgc
    16561 gctggacctg ctcgccgaga tggagaaaaa caggcgatgg ttcagcttct acatttcctc
    16621 gtccgccgag gccatcgccg cgttcggcat cgagaacatg gtccggctcg gcgtgaccat
    16681 ggtatggatc ggtttcgagt cgcagagcgc cgacgaattc gccaagaacg ccggcctcga
    16741 cgcggcccgg ctcgtccggg acctgcgccg gcacggcgtg tccgtgctcg gctcggcgat
    16801 gctctgcatg gagcaccaca cgcccgagaa catcggcgcc gacatcgatt tcgtggtcgg
    16861 cctgaacgcc gacatgatcc agttcatgct ctacaccggc atgccggtga cgcggctgta
    16921 cgaggatcac aagaggcgcg gcctgctgaa ggacgacctg ccgtacgagg aatggcacgg
    16981 gcagaaccag ctgaactggc gccacccggg attccccggc ggcgagccca aacgctggct
    17041 cgacgcggcg ttccggaggg atttcgaggt caactcgagt tcgatctacc ggatgaccga
    17101 gaccgccgtt cgcggatacc gcacgctggc cgcgatgacg tcgagggacg cctgcctgga
    17161 ggcccggatg cagcaactgc gccgccgcgc gcggctgtac tcgcacctgt tgcccgtgat
    17221 cgagcggcgc gccgtcaacg agaaggaacg gcgccgggcg atcgcgctcg acgcggagca
    17281 gcgggagatc ctcggtccga tccggatcac ggaacggctg acgcgcctcg gcgcgcgact
    17341 gctcgccgcg cggtgggatg tccgcttgcg cctgctcggc gaccgcctcc agcccgccac
    17401 catcgtgacg cgctatcgcg gcggccgggt gcgctgaaaa acaggcaggg cgccccgcgc
    17461 gggagacgcc ctgctgaatc catcaaccag ccggcctcaa cgccattcgc gacgaccgcc
    17521 gccgccgaac ccgccgccgc gcggacgttc ctcgcggggc ttggcctcgt tcaccgtcag
    17581 ggcgcgtccg tccatgtcct tgccattgaa gtcggcaatg gccttgttcg cctcttcctg
    17641 ggaggacatt tccacgaatc cgaacccgcg ggatttgttc gtgaacttgt ccatgatgat
    17701 cgtgcaactg acgacgcgac cggcctgctc gaacaggcgc tgaagatcgc cttccgtggt
    17761 gttgaacgac aggttgccta catacagttt cgtggccatg cgttttcttg ctttctgctg
    17821 cgggggagat ttcggtgatt gttcccgacc tgttcgggtt tcagatcgtc ccccgccgaa
    17881 acggtatcgg gcggctggac catccaccat ctcactgtcg aagctctctc tacaagcagg
    17941 ccgcagtgta ccgagtgccg ggaggaatac aaccggtttt ttcgggcacc cgggtaacgc
    18001 acagccctcc gccgcgccgc ttccgaccgg gtcattccgc ggcgggcggg cgttcgtgga
    18061 gggtgaagcg caccccggaa agaccgcatg gatggttccc gccccctacg gttgcgtgcg
    18121 cacgcggtac aggtaggggc ccgcggccgt ggccgtggcg tcggtgaccg acgtgtaatc
    18181 cgcctggccc gggacattcg cgaacacgtt cgtaaaggcg gaggacgaaa gcaggttcgt
    18241 ggcgcgatcc acgtggtagg tccggtcctt cacgctgcgc caccgcacga cgaggccggt
    18301 cgggccgccg gccagcggcg ggagggccat ccccaggtac gactcggcat tcgtcgggtc
    18361 cgtgcgcgcg ccgtattcgt cccggttgag cacgcggtcg ccgtccccgt ccgccgtggc
    18421 gctcatgccg gtgaagctgt gcgagtagtc ccattcccac cagtccggca ggccgttggt
    18481 gttggagtcc caggacgggg gcaccagctc gcaggcgccc atgtccacgc gggcgttgag
    18541 catcctgggt ttgccctcga cgtccagggc cccggtcatc caggcttggt tcgtgccctt
    18601 gtcgacgcac ggcgaatggg tggccagccg ccagtcgccc gcggccgcgt cgcgcagcgc
    18661 cggatcggcg gcgatgttgc ccggccccgg cggcagcgga acggcgcagg tgtaggcgat
    18721 gttcgtcgtt tcccactcat cgacgtacca attggtgccc tcgggcgcgc tgttgaacca
    18781 ggcgatgcaa ttcgacagga cgcagttggc cgcgccgccg ccgctctgcg aggccgcatt
    18841 gccgatcagc gtgcaattga cgagcagcga ccgctcggcg gcgccgcccc acaccgccgc
    18901 gttgctctgc atgacgcaac tgaacgccgc gccgccttcc acgccgcccc cgctgttggc
    18961 cgtattcgac tccaggacgc acccgtacag gacggactgc gcggccccgc cgccgccgga
    19021 tgccgcctgg tttccgctca cccggcagcc gtgcatgcgc gcattgtacg cgccgccgcc
    19081 ccagttccag gtcgagttgc ccgccagcac gcagttggac agcacgaccg ccccgccgct
    19141 gaccatgtcg cccgccacgc cgcccccgct ggcggcccgg ttggactcga tccgacagct
    19201 ttgcagcgtg ccccccatca cgcccccgcc gtcctggtag gcgacgttgt tcatgaccca
    19261 gcagttggac accacggact gggtcgagac gcagtagagg ccgcccccgt actccatggc
    19321 cgtgaacgga gaatcctcct gatgatccgt gcggccgccc gaaaccgtga acccggccag
    19381 gacggcccgg ttggtcaggt aggccccgcg cacggccgcc ggaccatatt ccccgtccgg
    19441 attaaaagga tccgcctgcc cgacgatgac cgtgtgctcc ggcccgttca cgctgcgcac
    19501 gatgacgaac ttgtccaggc tgactcgatt cgtcagggtc tggccgccgc acgggcgccc
    19561 gcccgtgtcg tagaccccgt tggaaaccca caccgtgtag cccgtgctca cggcatccac
    19621 cccggcctgg atcgttcgct tcgccgtggc ccagcggaac ccgtcgttcg catcgttccc
    19681 cgtcaccgcc acgtaggcgt tcgacgccac ggcgatgtgc acgatgccgg tgcccacggc
    19741 cccgctaagg agattcgaga ccagcacggt cacgactttc gtgcccggcg tcgagtaccg
    19801 gtacaccgcc tgggccgtgc cctgaccgga ggtcggcgcc ggcgaccagg aatagtacag
    19861 gggcggcgtc gcgcccggcg ggacgacgga aaccgtgttg gtgacgaacc ggttcgtcag
    19921 cgtgacggcc gggccggcca ctgtcgccgc cgtcaccggc aaggtcaagg gcccgctgta
    19981 ctcgtaggcc cccaggtcca ccacggcgtg ggcgatgcga ttggacccgt ccaggtccct
    20041 tgccgaagcc atccagttct ggtagagccc cgtattcacg caaggggaat tgctcgccag
    20101 gcgataattg ccgccggccg catcgacaaa ccccggcgca tcggtgaaat tcccgatctt
    20161 gggtgggagg ggcgtcgtgc aggaaaagta gatggaggtg gcaaagccat aactctcgac
    20221 ataccagttg ggcccttccg gcgcctggtt gtagtacaca atgcaattgt acagggtgca
    20281 ggacaaaacc ccgccgccgt catcggacac gatattcccg gccacggtgc agttgttgag
    20341 cgtgctccgg tacgcgccgc cgccgtagcc cgcggaattg ctgatcatca ggcagttcgc
    20401 gagcgtgcag ctgaaggccg cgcccccgta gtggccgtaa ttctcgttca ccacgcagtt
    20461 cgacaaggtc gagtacatcg ccccgccgcc gccggagctc gcgaagttgc tgacgacctc
    20521 gcagcctgtc agctcggcgt tataggcccc gccgccccag ttgtaggtcg agtttcggaa
    20581 cagcgcgcac cgcaagagct tggcgggcgg gccatacgaa taagatacgc cgccgccctg
    20641 gcccgcctcg ttgcccacga tccggcaatc ctccagccgc cccgcgtacg cgccgccccc
    20701 ttcccaggcc gtgcacccgg tgatgacgca attcgacacc accgcgttgc tgtacgcgcc
    20761 ccagaccccg ccgccgcaac cgtcggagcc gctgaccgaa ccggtggtgt gaccgttcgt
    20821 cagcgtgaat ccgcccagga acgccccgtt cgtcagggag acgcaccgga cggcgtcgat
    20881 tccttccccc cccagcccgg acgccggccg gcccacgatg aacgtcgccg cgggcccgtt
    20941 cacgctgcgc accgtcagcg gcttgtagat cgccacgcgg ttggtcagca ccgagtcgat
    21001 gatccggttc gtggtcccgc cggcgtcgta cacgccgttc gtcacccaca cgacatcgcc
    21061 gggattcgcc gcgtccaccg ccgcctggat cgtcgccttc gccgtggccc agttcgtccc
    21121 ggcggccccg tcgctgccgt tggtcttgac gtaccagtcc gccgcgcagg cggatacaga
    21181 gagcacacac cacgcacaga ccgccacctt ccgccatttg cccaaacgca tacgattcac
    21241 ctcgatgtat tcgtcggccg ggaaaaacgt gtttccgcgg cggcggcttg ggccaagccg
    21301 ccccgcccgg gaggcgttcc tgcccttgcg tcgctgaacg ggatcacgcg cgatatttct
    21361 ttgccggttt ctcgcggacg aggcttggtg ccgccaggtc cgccctgcgt ggtccggcga
    21421 accggacgcg ccgggagagc tgaagaactt ccacaccgac tacccgggcc cggcggtcca
    21481 ggtccacaat cacaccgggc gcgatccgct cggactcgat caccggagcg ccatcaagcc
    21541 gcaggtacag ggcgtcctcc gcccggtcta tcttcacctt cataacttcc ccttcatggc
    21601 tcgatcaaaa tataccgtga ccaccttctc gggtcgaacc gatttgttca cgaccacgcg
    21661 caaaacgcga tggccgtgtt ccgggatgca ggccagacga tgctccagcg cgcggtcttc
    21721 ttcatcaggc tccacctttt gcggttcacg caagacacgc tccaaccact cctccgcaat
    21781 gcctctctcg tgcaggacat gcgccgcgtg ccgggaaagc cgatacctca tgccccgccc
    21841 tccaccagcg cgatctcttc cccggtcagc ccgtagagtt catacaccag ccggtcgatc
    21901 tgccggtccg tcgcgtccac cgcgttctgt aacgtcttcc gcttggactc gctcgcctcc
    21961 gcccgcaact tcggaaccaa caccagcatc ttgtccacta actcgaccag cttgtcgtga
    22021 cgggcgcggt cagtcggcga agaatgccgt atgccagcaa caggaaatct ttcgacaaac
    22081 tgagatctga attcgaagta acacgcgccc ggaccctcca aacgcgcgca caccgaagta
    22141 aagaagaagt ttccaagttt tgagtttaga agtcccaaca gaaagcgcga atcggttggc
    22201 agaacgaaca gcttgtcatt taccaagtag cccttgtcat ctaaggcaaa gcgagattca
    22261 ggagcaatta tgggatagac gattttgggt ttcgccaaga gcggcaacaa tgcgaccgca
    22321 ggctgctgca gctcatacca ttcctggtga gcggcgcgtt gatttagcgc cgacttgaaa
    22381 cgctccaaat gcgtcttgac tgccgggaac tccttgggat gcccgccgtt gtgaaaatag
    22441 ataacgtgac gaccagaatc atcgatcgcg taccgcttca cgtcttttcc gaataaaaat
    22501 ggtttgatca cttcgtgatt ttttctgtca gtcttgagca gatgttgtct ggcgtccgca
    22561 tccaatacaa acgcctcgtt aaggcccgtt ttgattccga agagtggctt ccatccaaac
    22621 gcctcaacaa gatcgggaaa tctttcagaa agcttcgcaa ggaggtcagc ccgtccggct
    22681 ggaatcagtg cccaggggcg ttcggtaagt tggccgggcg ataagaattg ttcggcatca
    22741 tttgcgtatt gcgcaaccga taaccgaccc gactctattg attcaaagct agcagcactt
    22801 ggaacgggaa tgtatcgcgt ccgactagag ggtgattttt cattccgccc catcaccaag
    22861 acaatcgtac gcaccgttgc gcctttgaaa acacgcgcgc ccgcgaagtc agcaatttca
    22921 taaacgcagc ccatgttctt gatggttgac cgcaatggtt gcccatattt tgccaccgta
    22981 aatttattcg acacaatcat ggcgattagg ccggtatttc ctattaggct taacgtcttc
    23041 tctaagaagt agatgtagac atctgcgcgc tcttcatgag ctttgtagtg cgaacgcaga
    23101 tattttctaa tgggagcgaa ttcttccatg cgaacatacg gcggattccc gatcaccgcg
    23161 tcgaatcccc cacccttcat gatttcggcg aactggatgt cccagtcgaa ggcgtggaca
    23221 cgcatcaagt cttcggggac gagggagaag tccgaggcga tcagggagtt cccgtgcttg
    23281 atgttatttt ccagcggcgg caggagggcg gtgtcgtcgc gaaagagttc gcgctgctgc
    23341 ttgatggtct ggcggtcttc gccttcgagc atcttgaggt agagggagag ctgggtcacc
    23401 tcgacggcct gggcatcgag gtcgacgccg aaaatattgt tccgcaggat ctgccgcttg
    23461 aggtcgacgg cgaggtggac gtcgcccgtg gcgggatcgg tccagcaccg gtcgggcttg
    23521 cggtcttccg ggtgctccgt gaaccggcgc tggtaatgct cgccgacgcg ctcgaaggcg
    23581 cggaggagga agctgccgga cccgcaggcg ggatcgagga ttttcagggc gcgggttttt
    23641 ttgtcgaaga cggcgtaggt agggctttcg ggtagggcgg cttggcccaa gccgccgtcc
    23701 ctgcggcgcg ttgagccaac gcgccctacc ccggcaattt cgtcgagctg cttcccgacg
    23761 gtctgctcga cgatgtagtc cacgatgtag cgcggggtat agtagacgcc gccggccttg
    23821 cggacctcgg gcttctcgtc cgccgccacg ccgcggcccc tgggctgaat gaccttgccg
    23881 aggaaccgct cgtagacgct gccgaggatt tcgacgggga tgacgttgaa gaggtagggc
    23941 gattcgtcgt cgctcaagtc ctcgatgaag tcgcagagcc attggtcgcc gacgcgcagg
    24001 tcctcgctga agtggggctt gaacaggttg ccgttgaaat gcggcatatt gcccggcggg
    24061 cggcggtcca gggcctggag atggccgacc acggcggccc agagcgattc cgccgggggc
    24121 gcggcgcggt acgcggcgct cgcctcgcgg aagaaatgcc cctgcttcgg gcggcggcgg
    24181 tcctcgtggc cgtaggcgcg gcgccaggtc tcgacgatct tcgccagccg caggccggtg
    24241 tcgatgtcgc gatcctcgca gatgcgcagg aagaggagcc ggtcgaggat gtgttggacg
    24301 gcctcgttca gccggccttc ggccagcata tcctcgcggg tgttgttggc caggatgtca
    24361 cgggccaggg agaggcgctg ctcgtcgagg tattcgagga agtcctggtc cagggccttg
    24421 gtgcgttccg gacggatcag ccacggctgg cgcgccttcc ccttgacggg ccgggccttg
    24481 ggcaggctgt cgagcagcgc ctcgatgctg ccggcggcga cgcgctcgcg ggagagcagg
    24541 tcccatattt cgcgggcgca cagggaaaac tgccggaagt tccaggtctt ccagagcccg
    24601 tcgtcggggc ggtcggggcg gggccggccg ccgacaatgt agaccttgag gtcctcgaag
    24661 tccgtcagga tggcgagcgg caggcccttg ttccaggcgt agcgcttggc ctggaaggcg
    24721 taacgctcgc cgagggtttc gcgcggtttc ttggcctcgc agacgaagcg ctcgcggccg
    24781 tcggcgcgga agaggtagtc cgcccgcttg gcgcgcccgg caacatcggt gcggctttcg
    24841 atctccacct cgcgctggct ctggaccagg ccgcgccggt tcgcgaggtc ccagccgagg
    24901 gcgcggaaga gcgggttgag gaaatcctcg cggacgcggg cctcggagta gtccggcgcg
    24961 gtgaaggcct ggaagccctg ctcgaacgcg gcgaccagcc ggttcagttc gtcctggaag
    25021 ctttcgaatg cggctttcat atccccctta accggtcgga aatgaaccga ttatgtaacc
    25081 gctcggcccc gcgaagtcga gccggcttta ccggcgcagg gtccttcgca gagttcgagg
    25141 ggcgcggccg ttcgtacgag ggcgcggcgc gcggccgcgc cgcaggcggg acagtggccg
    25201 gaaggcggcc cgtagacgcc gatgagttcg gcgccgcgca acagggccgc gccgtcgcgg
    25261 cccaggaaag ccgagagccg aaggccggcc gcgaggaacg aaggctcgga gccgccgttc
    25321 aggaggtaca gcagcccgct ccgatgacgg cgggaggccg ggaccacgga ccataacccg
    25381 cgctcgttcc ggaaatcctg gaacggcacg ccgcgccgcc tcatccacgc cgcgaaggcg
    25441 cgggcgtcgc ctgcgaatcg gaccgcgaac acgcactcat ccgggtccgg cgccgcgccg
    25501 ccggggagcg gaccggccag gagttggcgc cgcgccgccc cgtcggcgtc ccggatctcc
    25561 gccgggccgg gcgtccaacc ttcgaaaaag gacgcgcgca caaacgcgag gccatgcccg
    25621 ggaaacgggc ggatcagctc gggcgagccg gcgtaccatc ggaccgcgtc gggccgggcg
    25681 gcgaagaaat cgcgcagcag ggcgcgcgcc agcgtgacgc ccggccgggc ggcgtccggc
    25741 ggcggcagcc aggcgaggaa aacaaccggc ctggcgctct tccggcttcg cggggatggg
    25801 cgcggtccgg cgccggacac cggcacagcc cgctcctccg tcggctcgtt cgacgcccgg
    25861 aggaattgcc gccagagccg ctcgcgcgga gtgccgcctt cgaaatcatc ccagggcaaa
    25921 atgtcctcct cgcggcactc ggccatccgc cccgcgacat cccgcccgct gtcgcgcagc
    25981 caggccaggg tctcctgcag cgccgccggg ggcaggtgct cgtcgtagcg cagcccggaa
    26041 tcgagcgaca gcttcacgag cagcggggtc gccgcccggc ccaggcggca caggagattc
    26101 gtccattgaa tctcgtccgg ccacgcgctc cagcggaact ccgcgccgcc cgcgcgggcc
    26161 gccgcctcgg cctcccggat catcggcccg gaaagcggga tgatctccgc gaattgcagc
    26221 ggcgtaaacg gcgcgtggaa cagcggggtg aaactggcgg tgagcgcggc ccccggcgcg
    26281 gcggcccgaa cggcgccgag gagccgggtc agctccgccc agtccgccga ttcctcgcga
    26341 cccgtggcga tgaagaagag cttgatccgg cggagcccgg cgggcgccag ttcgcggacg
    26401 acggcctcga gttctgcgtc ggacacgggc ttgcccagcc aggcgcgcag ccgcgcgctg
    26461 accccttcga ccccgaaggt gaactcgcgc ttgtccagcc cctgcaccag cgcgcgcagg
    26521 tcaccccggt gcgcgaggct gaagagggag aggctcttga ccgaaagccg cgggaagatc
    26581 tcctgcagcg gcgggatcaa ggccgccagg cgcccgatgc tgcacgcgtc cgaggccagc
    26641 aggttgaggt ccgacgcggc catcgccgca cgcagccgcc gggcggcccc gaggaacccg
    26701 gccgggtcgg cgacatccag cgagccgcgc atccagccgg tctggcagaa ccggcagcgg
    26761 tacgcgcaac cggccgccag cggcagcgtc tcctcggtat cttcgtccga gaacgggata
    26821 aaggcgccgt cgtagccgtt catccacgtt tcggccgggt ccttgcggta ggtcacacgc
    26881 gccggcggct cgtcgcggag cggacggatg gcggcccgcc gctcgccgga atactcgtgc
    26941 cggtagaagg cgggcacgta ggttccgggc aggtctcggg ccaggcgcgc gagaaactcg
    27001 ggcctgtcca tgccggttgc ccgggcctcc gcccacgcgg agaggacccg gggcagccag
    27061 gtcagcccgt cgccgaagca gaccgcgtcg accagtccgc ccgagtcctt cccctccgcg
    27121 tcgccgtgca gaaacggcgc ggcgcccgcg gcgttgccgc cgagcaaaac gagcggatgc
    27181 ctggcatcag ccgcccgcgc ggcccaggac agcttcaggc ccgaccggcg catcgcggcg
    27241 gggagattca gggcctcctg ctgaaccgac aggctgatcg cgaccacgtc gaaccgggcc
    27301 ggcggctgcc tggacgcggt tccgaaccac cacgggacgc cgtccctggc cataagcgcg
    27361 gcatcgcgcg gaggcggcca gaaggccaga tcgacgaagg cgccgtccgc gttccgggcc
    27421 gcccagtaca gcatccggtg cgtgatggaa tgcagcacgt cgcggtagga ggagagccgt
    27481 gcgatcagca cgcggacgcg ggcggcatcg aacaggccgg gctccatgcg gcccggttcg
    27541 ccgccgtcga gtcctatccc gccgccggcc agctcgccgc ggcgcgccgc gatccagcgt
    27601 tccagttcct ctcttgtgcc cagcatcacg ggctcaccat acctgtctct tcggaaataa
    27661 cccagcccct catggggtcc tcgaatactc gcttgtgaac aggaggcaac ggagaaagaa
    27721 gcggcaggag cgacgccctg cttgcaacgg tcgaggacct gccccatgat gaccgcaaga
    27781 ggaagatcaa ccccgagata cccatgaaaa tcctgatcgt cgataaattc cccgaccgct
    27841 acatcgccgc catccgcaca ctcggccacg acgtcgcgta cgagccgggc acccgggcca
    27901 cggacatcgc cgcgaagagc ggcgacgcgg acatcatcgt ggtgcgctcc acggaggtga
    27961 cggcggaaac cattcaggcc tcgaagaacc tgtccctgat catccgggcg ggcgccggga
    28021 cgaataacat cgaccggaaa gcggccagcg ccgcgggggt gtacgtgtcc aactgccccg
    28081 gcaccaattc cgtggccgtg gcggagctgg ccatgggctt gatctgcgcc ctggaccgcc
    28141 ggctcgtgga gaacgtcagc gacctgcgcg cgggccggtg gaacaaggcc gagtactcca
    28201 aggccgccgg actgctcggc aagaccctgg gcgtgatcgg cgtcggcatg atcggcaagg
    28261 agctgatcgc gcgggcgcgg gcgttcggcc tgaaggtcaa ggcctggagc cgctcgctga
    28321 cgcccgagaa ggccgaggcc ctgggcgtgg aatacgcggc ctcgattgcc gcgctggtgc
    28381 cgacctgcga catcatttcc gtccacctgg cccaggcggc ggaaacgaag gggatcatct
    28441 cgcgcgaggt catcgcgacc atgaagcccg gggccctgtt catcaacacg gcccgcgcgg
    28501 gcgtcgtgga ccaggcggcc ctcgtcgagg cggtgaaggc gaagaggatc cgcgtcggca
    28561 cggacgtgtt cgagggcgag ccggaaggca aggacgggcc gttcgccgac gaactgggca
    28621 tgctgcccgg cgtgtacggc acccaccaca tcggcgcctc gaccgagcag gcccaggacg
    28681 ccgtggccac ggaaaccgtg cgcatcatca aggtgtacgg ggagaccggc cgggtcgaga
    28741 actgggtcaa caagatgaag aagaccccgg cccgcttcca gctggtcgtc cgccactacg
    28801 acaagcccgg cgtcctcgcc gacgtgctcg cggccatccg cgaggcgaac gtcaacgccg
    28861 aggagatctc gaacgtgatc ttcgacggcg cgcaggcggc ctgctgcacc attcaactgg
    28921 attccgccct cgacgccggc cggctggcca agatcaacgc gctctccggg cgcgtgatcc
    28981 acgccgcgca ggtcgccctg gcgtagcctg gtccgcctgt ccaggggggg ccgcggaggc
    29041 cccctttttc catcccgccg taatcgtccc gctcattcga tggatgccga ctggatatat
    29101 ttatatatag ccatatggct atatatgaat acagcgtatt gtaaaacgac gcaagggcgt
    29161 gaggtcgaag agcagccggc cggaggggcc gcgtaaaaaa agcgcccgcc cgtgggatcg
    29221 cgggcgggcg ttaaggggtt ccgttcggcc tcgggtcacg caccgctcct cggtcaggtg
    29281 gcggttcatg tggtcgccca ggagcgtgac cacgcggagc ttccggccct tggccttctt
    29341 gatctcctcg agggcgaagt ggacgttggc gccggacgac acgccgcagc acagccgctc
    29401 ctccttcgcg ggaaggtata aattttgaaa acacggcgaa agaggccggg aaagccaacc
    29461 gtggaccagc ccggcatcaa tccagccgct tgcggaagtt gaggacggcc aggcccagga
    29521 tcgcggcgcc ggagcccagc agcgcgaggg cgtccggcca cagcctgcga agcccgaccc
    29581 ccttgaggaa gagcccgcgc acgatgcgga gatagaactt cagcgggatg cactcggcga
    29641 cggcccggat tggcgccggc atgttctcga ccgggaaggt gaaccccgag agcagcacga
    29701 acggcagcat gatgaagaac gtcgccacca gcatcgcctg ctgctgcgtc ttcaccagcg
    29761 tggacaccag caggcccagg cccaacgtgt tgagcaggaa cagcccggac ccggcgtaca
    29821 gcaggaccag gcttcccttc aggggcatgc ggaacaggaa cacgatcacg gcgacggcca
    29881 gcgtgacctc ggcgaacccg atgccgacga acggcagcag cttgcccgcg atcagctcgc
    29941 tcggccgcag cggggtgacg atgacctgct ccatcgtccc ctcctcccgc tccttcacaa
    30001 gggccatgct cgtgatcacc atggtcgcga ccatcagcaa caggcccatc acggccggca
    30061 ccatgtagcg gcgggagtcc aagcttggat tgtaccaggc gcgcgactcg gcggtgatcc
    30121 ccggcgcgga ggcccggccc cgccgcgcgg cgtccgccgc gtccgcgcgg accagtccgc
    30181 ccgagtactg gcgcagcgcc ttctggagat agttcaggcc caggacgccg aagttgctgt
    30241 cggagccgtc caccagcgcc tggacccgcg cgggccggcc gcgcaacagc tgccggctga
    30301 agtccgcggg aatgaccagg acgaggccgg cgtcgccgtg gaccaggcga tccgcgtcgc
    30361 ggacctcggg cccgtccagc atcgtcgtca ggaaatagcc cgaggaggac agcgcgcgta
    30421 cggcctcgcg gctgtggtac gagcggtcgt tgtcgcgcac ggccagccgg atctcccgga
    30481 cgtccgtcgt caccgcggac ccgagcacga ggagctggag gacgggcgcg acgatcagca
    30541 gcccgaggat ccgcggatca cgccggatct ggatcacctc cttggccagg acgctccgga
    30601 tgcggttcat gaccagaggg ccttccgggt tttgtgcgcc gccagcaggt tgaacgccac
    30661 gcccagcagc agcatcgcca gcaggtccat ccacaccgcc tggaacggcg cgcccttgag
    30721 gatgatcttg cgcagcgcgg acacaaagta gcgcggcgcg aggatcagcg tgacgccctg
    30781 gatcggcagg ggcatgttgc ggatcgggaa aatgaagccg gagagaatga tcgagggcag
    30841 cagcgaggcg atgacggcga cctggaaagc gacctgctgg gagcgggtga cggcggagat
    30901 cagcacgccc atgcccagcg ccgcgaaaag gaacagcagc gtcgccaggc cgagcagcag
    30961 gaacgagccc cggatggcca cgccgaagag cacgtagccc agcagcagca ccatcgccat
    31021 ggtcgccagg cagatcagca cgtacggaag cgtcttgccc aggaccagtt cctccggccg
    31081 cagcggcgag accatcatct gctccatcgt ctccctctcc cgctcgcgca cgatggagag
    31141 cgacgtggcc accacggcgg agagcatcag cagcagcgcg atcaggcccg ggacgaggaa
    31201 cttcgcgctc tcgagctccg ggttgaacca gacccgcgga acggcgatca cggccggccg
    31261 cgcggcgggc gtcgcccgca agtccgcctg cgcctcgagc gcggcgcgct cggcgagggc
    31321 gtcgaggtag ccgatcgtcg tggcggccgt ggtcgcgtcc gcgccgtcca ccagcgcctg
    31381 cacgtccgcc gccccgccgc gcgcgaggtc ggcggcgtaa ccccgcggga tgaccaggac
    31441 cgcccgcgcg cggccccggt cgagcacggc gtcggcttcc gtcgaggagc ccagcgcgcc
    31501 ggcgcggtcg aagtagggat tctcgaaaag gccatcgagg aagtcccggc tctccggcgt
    31561 gcggtcataa tccgccgccg cgacccggat gtgcttgacg tcgaaggaca gcgcgtagcc
    31621 gtagagaacc aacagcagcg ccgggatgaa gatcagcagg ccgagcgaca gcgggtcgcg
    31681 cccgatctgc cggaactcct tcttgaagat ggcccagaag cgtttcacgc ggcggcctcc
    31741 cggccggtcg ccgccagccg gatgaacgca tcctccagcg ataccggcac ccgctccacc
    31801 gagttcacgg tcatcccgcc ctccgccaga gcgcggccca ggccctcggc cgtcagttcg
    31861 cccggccggc cgaacacgtg caggtgccgc ccgaagtacg acgtggccac gacgcccggc
    31921 accgcctcga cccgctcgcg cgcgcgctgc atgtccgcca gcgagacctc gtagaggtcc
    31981 tcgcggacgg cgtcgcgctt gatggccgcg ggtgtgtcga gcgccacgag ccggccgccg
    32041 ctgatgaacc cgatgcgccc gcagaactcg gcctcgtcca tgaaatgcgt ggtgaccagg
    32101 atcgtcaccc cgcggccggc gaggtcctgg atcaggtccc agaaatggcg gcgcgagatc
    32161 gggtccaccc cgctcgtcgg ctcgtcgagg aacaggatgg gcggctcgtg caggatcgcg
    32221 cagccgagcg ccagccgctg ctggtgcgcg ccggagagcg tccccgcgag gcggtcctcc
    32281 aggccgtcca gcccggcgag ccggacgacc gcgcccgcgc gctcgcggag ccgcggccgc
    32341 gccaggccgt agacgccgcc gaagaacgcg atgttctccg cgaccgtaag gtccttgtag
    32401 aggctgaact tctgggacat gtagccgatg ttctgccgga cggcctccgg gtcgcggttg
    32461 atatcgtacc ccccgaccgt cgccgtgccc gcggtcgaag agagcaggcc gcagagcatg
    32521 cggatcgtcg tggacttgcc cgcgccgttc ggcccgagga acccgaagat ctccccgcgc
    32581 tccacgttaa agcgaaggcg gtccacggcc gtgaaccggc cgaagacctt cgtcaggccg
    32641 tccacggcga tcaccggttc tttgccttcg cggttcacgg ggatccgggg ctagggtttc
    32701 ggggcggcca ggataccgac ggcatgatcc agcgcggcgc gcgcgacgac cgcgtccgcg
    32761 cgggcggaca cgagcgcgaa ctcggcgtcc gtcagctgcg catgggcgtc cagcacgtcc
    32821 gcgtgccggg ccaggccgtt ctgccagaga tcggtggcgg actgcaggtt gagcgcggcg
    32881 ctgtcccggg cgcgccccgc caccaccgcc cgttcgcccg cgttcgccag ggcgacccgc
    32941 gcctgtcgga cctcgagcgc gacccggtcc tcggcctgcc ggcggcgcag gtccgcctgc
    33001 tcggcgcgcg cccgggcctc gagcacccgg ccgcgggtca ggcccgaatc gaagagggcc
    33061 caggacgcca cgacgcccac gaaggcatcg tcctgccact cgtcctcggg cgggaagatg
    33121 agagtgttcg ggcgcgcctg ttcatagcgg gcggacagcg cgagttcggg cccgtagccg
    33181 gcgcgcgccg cgcggaccgc cttcgccgcc gatcgggcct cgagcgccgc ggcccggacc
    33241 tcgggccggt tcgtggcggc catctccagc aacacggact ccggcgcggg atccgccgcc
    33301 ggcgacgcgg ccgcctcgtc cgcagccgcg ttcgacggca agctcgcgcc ggtcaggaaa
    33361 tccagcttcg cgcgggccag ggcctcgttc cgctccgcct cggtccgctg cagccgcgtc
    33421 cgttcgagca ggacctccgt cgagagtcgg tcgttatccg tcgccaggcc cgcctcgttc
    33481 atgtttttca tgtcccgcgc atgggcttcc atgcggtcga cggacgcccg cagcgcgcgc
    33541 agggcgaaga aggcccggga ccacgaccag tacgaggaca gggcctcgag gaccacgtcg
    33601 gactccgtgc cggccagcgc ctgccgggcc gcgtcgctct gcagcgctgc ggcctgcttc
    33661 aggctggaga cgcgcccgcc ggtgtagagc ggctgcgaga cgccgatcac ggcgccgtag
    33721 cggtcgggga tctcggggat gatcatggtc gggctcaagg ccgtttcctt caggccctca
    33781 tagtgcgccg cgcgcgcctc ggcgctgacg gtcggcaggc cctgcgcccc cgcctgcgcg
    33841 agcgacgcgg cggcggcccg tacgtcctgc tccgccgact tcaggcgcag gctgtacgcc
    33901 agcgcctgcg cgacgatcgc gtccgcgcac acgggctcgg gcggctccgt ggcggcgaat
    33961 cccgccgggc cgagggccag gaataacaac gcgcatttcg ctctcatgtc gaactccctt
    34021 ctccgagaag gccgacgaag gcgtcctcca gcgagggcgc cgcctcgctc gcccggacgg
    34081 ggccgcatcc ctccgtttcc agaagccctt tcgcctcggc gagggcggac gccccctcgc
    34141 gaaacgaaag ccgcaaccgg tcggcgtaca gcacgacatt cgccgacgac caccggcggc
    34201 gtaaaacgcg gtaggccgag gcgggggccg ggcaggcgac ctcgtagacg cggccgggca
    34261 ttgccgcgcg gatttcggcc ggcgtgccgg tcatgaacag ccggccgccg tgcatcaggc
    34321 cgatccggcc gcagcgctcc gcctcatcga ggtacggcgt ggtcatgagg atggtcacgc
    34381 cctgcgcgag gatgccgctc aaaatgttcc agaactcgcc gcgcgagacc gggtccacgc
    34441 ccgtcgaagg ctcgtccagc aagatgagct cgggccggtg gatcagggtg caggccaagg
    34501 cgagcttctt cttcatgccg cccgacaggg cctgggccag gcgccggcgg aagggcgtca
    34561 ggcgcgtgaa gtcgagcagt tcgtccctcc gcgcccggaa cccgcgcacg ccgtggaggt
    34621 cggcgaagaa ttcgatgttc tcgtcgatgg tcaggtcgcc gtacagggtg aagttctgcg
    34681 agaggtaccc gatgcggctc ttgatcggcc cggcctcgcg caggaggtcg aaccccagca
    34741 cgcgcgcctc gccggcgtcg ggtttcagga tgccgcacag caggcggatg gccgtgctct
    34801 tgcccgcccc gtccgggccg acgaggccga acatctcgcc ccggcgcacg agcacgtcga
    34861 gccctgccac ggcgcgcgtc tcgccgaacg agcgcgcgag cccgtgcgtc tcgatcgcgt
    34921 cggctgaatt tcccgtttcc atttttattt ttccaggtaa ccgtccgccg gcatgccggg
    34981 cttgaagaca ccgtccgggt tcggcagtgt gatcttcacg cggtagacca gcttggcgcg
    35041 ctcgtcgggc gtctggacgt tcttcggggt gaactcggcc tcgggggaaa cgaaggtaac
    35101 ctttccctcg aacacgcgcg cgtcgccgtc gatgcggacg cgggccggct ggccgagctt
    35161 cactcggccg aggcgggtct cggggatata gacggagagc catacctcgt ccaggcgcga
    35221 cagggtcagg agcgcggcgc cgggcccgac gtactcgccg gcctcgcgga ccttcgtggt
    35281 gatgacgccg gcgatcggcg cgatgatcac gcagtcggcg acggcctttt cggcctgggc
    35341 cagccgggcc ctggccagat cggcggcggc ctgcgcggcg cggatttcct cctgccggtt
    35401 gccgcggacg agtttgtcca ggttctgctg cgcggccgcc agcgcggcgg ccgtgcggtc
    35461 ggcggcggcc tgggcgtcgt ccatctgctt ctgcgtggcg cttttcttat cgaacacctg
    35521 gcggatgcgc acgcggtccg cctcggcggc cttcgcggcg gccccggcct cgcggacctg
    35581 ctcgcgggcg cgctggatat cctcgtcgcg ggcgccggcc aacatcaggt ccagttgggc
    35641 ctgggcctgg gccagcgccg cgcgggcctc gtcgcgcttg agctcgtaat cgacgggatc
    35701 gagcttcgcc acgagggcgc cctgggccac cgcgtcaccc tcctgccccg gcagctcgac
    35761 gatccggccc gcgacctgcg gggacacttg gacctgggtg cactcgatcg tcccggaccc
    35821 atccggttgg ccgtcacgca tcccgcaccc ggccagcacc gcgatccccg ccgccaccat
    35881 cgtcacgcgc gcccgaattc gtttcatgct ccccgattcc ttcccgcggg cgaacggccc
    35941 ccgcgctttt ccaccacgcc gtgcaacagc aacccgagaa tctgttcctt gcgctccgcc
    36001 gggcccgcgg acggccgccg gatcggaacg ggccccatgt gccggaccac ggtctgcacc
    36061 ttcagcgaga acaggcacat ggccaggatg ctccaccagg cttcgacggg gtgcatgcgc
    36121 cggagttcgc cgcgccgcat gccctccgcc agcacctcca agggcacgag ttcatggccg
    36181 gcgacgagcc ggcggagggc gcggccgaat tcctccgggt gccggctgac cgccagccac
    36241 atgaggtcca tccgctccgg gtgccgcgtg tagtagtcga agatgccgtc cacgaggtac
    36301 cgcagccgct ccgccgcgcc gcggaaggtc cggcgccgtt ccttcaagtg ggccaggaag
    36361 cgctcgagaa cccgcgacac ggcctcgacg aacagggcgt tcttgtcggc gaagtagtag
    36421 tacaacagcg cgctgttcac gcccgccgcg cggccgaccc cgcggatcgt cgcgcccttc
    36481 agaccccgct ccgcgaaaac gcggatcgcc gcctccagga tcctcgcccc atgtggtgaa
    36541 tcgcgccgcg ccattgttgc ctcgcgcgat ttaatcaatt gattaaatca tacctcgagc
    36601 atacccccgc acggtcaggg atcaagaggg aaagcggatc tggcatcagc ctgccagagg
    36661 attgggacca ggaaagcgac gaatccccaa aagagcttcc cctcttgctt cttcgagaac
    36721 aaaccgaata gtgttgcagt gtccggccga gccgggggat cagactacag atagtctagg
    36781 cgatggcgaa tagcagccca agtatccttg tcttccttag actgtgacag tgatctttcg
    36841 tagccgcgtt caatgaaata cgtggtcatc agagtagtca gaagagacac aggtttctcg
    36901 tttctcttgg gctgattact ggcccatgct agcgcggcga catagtccgt ctgcttcgca
    36961 tagacttgaa tgaagctgga cacccaagga atcaccgctg cgaagaacga caagttactt
    37021 tgcccacttg gaggcccagc gtcacgatga gagtcagcga tagcgctcaa gtatgcgacc
    37081 tctttctccg cgatgatgga ggacaatcgc agcttcttct ccacggcggt cttggcgaga
    37141 gctgccatct cctcgcgttt cagtcggctt ttcggtcttg cggtcatttt ctacctccag
    37201 ctaaaccaca acctccacta ggaggaagct ccgtccttaa tctccgaatc agtgtcccat
    37261 cagaatccgt ggttgttcca tacgtgaata gatgggcaac atccagactc ttggttttgc
    37321 ccgcgatcca tgagtacgtc ttgaagcgcg tcttctgtga aatgcccggg gtagggtaat
    37381 tcggaactgg attctcgaaa ctttgtaatc cggtgttgca ggcgaatcca tatgcgaagc
    37441 gcagcgcaaa tggggaatcg tggagccact gacaagtgaa ccatccttcc tcaaggctgt
    37501 ctttgttggg cttcttctga ggcgcgaggc caaaatccat gcacgcgaga aactgggctt
    37561 gaaactcgat cgcatcgaca acaccgcttg gagtgtccat taccacgtcc atatgttcaa
    37621 gcacgtagtt tgccatgttc ttccaagtca gcgtacggcg caaacggcac cgagacaatc
    37681 cgttaatacc gagcaagttt ccatcaagag gacaatcaat aaaaaccacc tgatcgctgt
    37741 tactaggcgg ctcaggcttc tcatccctgc gaatgtactc aagattgaaa acgtccacat
    37801 cgacgggcgt atcggtaccg agccgggagt caaacagagt caggcgctga atttgcatgc
    37861 agcgcttttc aggctcatga ccgtctggat tgaatcgggt catgtggtgg ttaaaaagat
    37921 caagcatttc gagtcggtgt tcgtagaaat ggtaccgtac gctgttggcg gctcccgcaa
    37981 aagtccattt gctgcggtct ttctcggcgg ggccgggcgc ggcacccaag ctgaacgaca
    38041 ccgttagcct gccgttgtca acttcgactc tctgaccttg tacctggggc gtttccattg
    38101 tcgagcaatc tcagactgat tgcgttggtg gtgcaggatt tcatgcctgt gtgtgcacag
    38161 tcaaggagaa atcgcgatag gagaaatcgt ctcacgagtt ggctcccttg tctttaggac
    38221 atgttgccac aactgactga tttctttccc ctcggcatcc gttgtagctc cataaccgaa
    38281 tcgatgggcg actatctggt ggtcgcaacg gttggtgctc catgaatatg tcttccacgc
    38341 aaatctctct ccgcccaatg ctgccctagc gcaatggttg gatgtctcca tatcctttgc
    38401 caatggagcg ctggatgcaa agccatagct aaaacgaggc gcgaaggtgt tgtcatttac
    38461 gttggcgatg gtgaaccacc catccatatc cgttcttttc gctggactcc tgctcatgtc
    38521 aatgtagcca aagaagtcta gataaaacgt aagcctcttc gcgccttcca ctgacgggct
    38581 ccggtcggtc agcgaaatgt cctcctgaaa atggtaatcc ccctgcttca ctgtgaatcg
    38641 cctattcagg aacaagacgc gccgggcatg ggggcggcgc gtaccagtcg cttgaaatcg
    38701 acattgaata acaatactcc tctcaggccc cacatccttt ctgccaatca agacgtgctt
    38761 ttggccgaac aagtcaacgg gacggaaacc tagaggtctg attcggcgaa tctgcataat
    38821 tctcgttcga agatccgggg gggcataact gccgctcaaa acaacaagtt ggtggaaaat
    38881 gtccagcatg ttgtggtcgt cgagcttcac gctcgttgca gcacctgcga aatagctgcc
    38941 gaatcgtgct gcctcggtgt gatccagttg agggttcagg ttgaatttgg cggtcagccg
    39001 accatttccg atctcgacac tagccgtcgg ttgaacactg gggttggcac gcgccctcac
    39061 cttggctttg ctgatatctt cattggctga tccgatgtca gcacttcttc tgcccacctc
    39121 gcggcgcgga ccagcattcg gctccggtcg agtacttctt cgaacggggc ggcttatcct
    39181 cacggcattc gaaaggccac cagcgcttgt cgggaggacg ggcgggagtc ggacttctcc
    39241 tgcttccaat cggtgtagaa agtccaaagc cattgccgca cctgtcagag caacgcgaca
    39301 ctttcccacg aactgctgaa cactggggaa ggccaaggcg cgtccatgaa ggaaatagag
    39361 ggcatgcatg taatcgccga gcacgtactc atcctgatcc gcgcccagaa tctttagggc
    39421 ttcacggcgg atggcgttca caaacagcac ggcattccgc acgagcggag ttagtgactt
    39481 gatgtccccc tccccgaaat ccagcatgcc tcgcgccgta accagatcct ccgccatttt
    39541 gccttcagta agcagtgtcg gcaggatttc atgccagaat tcggtttcca gcctcgcaaa
    39601 gtcaatcgcc gtcggggcgg caggtcggca gttcgcaaga tcaataataa acgcattcgc
    39661 cggcgcgtgg ttcggtgtga gtccaatcaa gagattgccc aagttaaggt caccatgaat
    39721 acgggataca cgcgtgcgtc cctgcggcat agccgcacga ccccagtatc cggcaagtgg
    39781 attcagcagg ttcctagtgc cggagagaga gagatctgga ttcctccaca aggccgggga
    39841 gtgcttgcct cgcccccttg agcattgccg ttctgcatgc agcctttttt ggcagccaat
    39901 ccttccaaca cagatgccgc cctccggcaa tgaaacgtag ggcaccgggc tcgctaagat
    39961 agaacggtcg tagcgcgtca aagcacagat ccaaggcttt gagacagttg ttcacgttgg
    40021 tcgctaactg gcattgaaga agctcctgaa gagtgctcac ttcgcctgaa ggcgtcggga
    40081 tgtttgcgtt ctggtacacc acgacgccgt gttgcttgcg gttcttggca acgggaagca
    40141 gcgcttgggg cggcgtattc aaccctcgca gttcctcgat tcgctgccat tccttcttgg
    40201 cacgatccgg ttcatcgaat ttggcaacta tctgcagctc cgcaccattc ggacgcggaa
    40261 tcacgagaaa ccaaactgcg gaacgctcca gcacaccgct cctgcgggaa tacggattcg
    40321 gcagtacggc gcttctcccc aggagtttca tgtccaccaa ggccgcatga aggatctcgc
    40381 gccgtgagct gtctaattct ttagtcatta atttggagtt gtttttacga actaacgtgt
    40441 cggcattcaa tctttattag ggctatgcac ttgactcatc gcatccaatg ggttgtagca
    40501 tctccccatg tccgagaact acccgacacc cttgttggac ttggaggaac gatttgcttc
    40561 ggaaaccgcg tgccgcgagt acttggagcg gctgcgatgg ccgaacggat tcatctgccc
    40621 ccagtgcggg gtggcgcggg cggggcggat gcagcgcgga ctatggcatt gtcggggttg
    40681 ccatcggcag acctcggtct tggcgggcac ggtccttcag gattcccatc ttcccgtgcg
    40741 gatctggttc cgatccatgt ggcatatcac tagccagaag aacggggtca gcgcgctggg
    40801 cctgcagcgg gccttgggct tggggagtta ccgaacggcc tggttgatcc tccacaagtt
    40861 gcggcgggcc atggtgcggc ctggccgcga gcgtctgcac ggcgtggtcg aggtcgacga
    40921 ggcctactgg ggcgctggag aagaaggcgt gatcggacgg cagaccgaaa ccaaggcgct
    40981 ggtgggcgtg gccgtcgaag tgaaagggac gggcatgggg cggattcggc tggccacttt
    41041 gccggacctg ctgggcacca ccctacagcg ctttctcgac cacgccgtgg agcctggcag
    41101 taccgttcgg accgatggac tgcccgcgta tgggggcatg aagggctacc gccatgaacg
    41161 ccaaatccag aaccggcaac cggcgggcga tcatctcttg ccccacgcgc atcgcgtcat
    41221 ctccttgctc aagcgctggc tggtcggtac ccatcaaggg gcggtctccc acgagcatct
    41281 cgacgactat ctcgccgagt tcaccttccg cttcaatcga cgcatgtccg catctcgcgg
    41341 caaacttttt tatcgattgg ttcaacaagt ggctcaggtc tccccacagc cttacgagat
    41401 ggtgacgaac ccacagccga ttgtgcctga tgagtcaagt gtatagccct attaccgatt
    41461 ggcccggcca cgcgtgcgtg ccgagatgct tgccggtcgc cccctcgccg atgacgcggg
    41521 tcagctcggt gtaggcgcgc acgtcgtgcc gcccgatcag ttcgcggatc tcgccctgcc
    41581 gcgcctcggg agagatgatc atcaacagtt tcatcgtctt ctccttcctc gggagggcgg
    41641 gcttccacgc ccgcctcccg gcgcgggtgg aaccgcgccc tcccctcaca cgcccttctt
    41701 tccgtaaatc atcgcgtaca ccacggggat cacgaccagc gtgaacgcgg tggacacgaa
    41761 cagcccgaag atgatggacc acgccaggcc gctgaagatc ggatcgaacg tgatcggcca
    41821 ggcgccgagc atcgtcgtgc ccgcggtcag gaagatcggg cggaaccgca cggcgccggc
    41881 ctcgatgatc gcttccttca aatcctgtcc ccgctccgtc gcgctgcgga tgaagtcgat
    41941 caggatgatc gcgttgcgtt gcacgatgcc gccgagcgcg atcatgccga tcatcgccgt
    42001 cgcggtgaag aataccggat cggcgtaccc gccgatcgtc cggcctgtca gcacgttgag
    42061 cagccagaag cccggcatga tgccgatcag cgtcagcggg atggacagca tgatcacgac
    42121 cggcagcagg tacgaaccgg tctcgtagac cagcagcacg tagatcgcca ggagcgccgc
    42181 cgagaacgcg aggcccaggt cacggaacac gtccagcgtg atcttccact ccccctcgcc
    42241 cgtccagctc gcctcgattc ccgacggcag cgggttcttc ttgagccagg cctgcatcgc
    42301 cagcacggcg taggcggggc cgcgcccggc catctcggcg gtgacgtaga ccacgcgccg
    42361 catgttcttg tggaagatcg tcttgtcgta gacgttctcc tcggaccggc cgatctcgcc
    42421 gagctggacg ctgttcccca tccgcccctt gacgggcagg gtcagcaggc gcgccggatc
    42481 ggaccgcttt tcgcggggca accgcaggac gatcggcagt tcgttctgct ccgtcggcac
    42541 gtgcaccgtg cccaccggga acccgctcat cgccaggcgc aggctgttga cgacgtcctc
    42601 ggtcgagatg ccgttcagcc cggccttctc acggtccacg cggaagaaca gcttctgctg
    42661 gtccgcctcg accgtgtcgt ccacgtccac cacgccctgc tcctcgcgga gccgggcgcc
    42721 gacggtcttg cccgcggcga tcagttcgtc gtagtcgtgg tgcgggccgc cgtagatctc
    42781 ggccacgacg gtggaaagca cgggcgggcc gggcggcgcc tcgacgattt tcacgagcgc
    42841 cccgtgccgc gccgcgagcg cctcgacgtc cttccggatc cgcagcgcaa tgccatgact
    42901 ggccatctcg cgcttctggc ggggcagcag gttgatccgc acgtcggcga cattcggccc
    42961 ctggcgcagg tagtaatgcc gcacgaggcc gttgaagtcc atcggcgaac cctcgccccc
    43021 cgtcgcggtg aagtccacga cctccggcac ggtccggagg tagtcctcca ggtcgcgcac
    43081 cacggcgtcc gtggcctcca gcgtcgcgcc ctcgggcaga tccacgacca gctgcagctc
    43141 gttcttgttg tcgaacggca gcatcttcag cggcaccagg ccggtcgcgg ccagcacgat
    43201 ggagaacaca aacaggccgc ccatcacgag cagcagcagg tttcccttgc cgcgcgaatc
    43261 gaggaacgga cgcacgagcc ggtcgtagac gcggaaggtc gccgtcgcct tgacgtcgaa
    43321 ggcagccgtg tcggcggacg gctccttgag cagcttgttg gagacccacg gcgtgatggc
    43381 cagggcgacg aagagcgagc tgatcatcgt caacggcacg ttcaacgcca tgggggccat
    43441 gtaggggccc atcatgccgg tgatgaagaa catcggaacg aacgacgcga tgatggacag
    43501 cgtcgccagc acgatcggcg gcatgacctc ttccatcgcc agggcgaccg cctgcaggcg
    43561 cgggaagcgc cggagggcga ggtggcgcga gatgttctcc acgccaacga tcgggttgtc
    43621 caccagcagg ccgagcgcca ggatcagggc gaacagggtc acccggttga tcgtgtagcc
    43681 cagcaggtag ttgaagagca gcgtcagcga gtacgtgatc gggatcgcca gcccgacgat
    43741 cacgccctcg cgccagctca tggccagcgc gatcatgccg acgacggtga tcaagcccat
    43801 gaccaggttc atgaccaggt cgttgacctt ctcgttggcc gtctcaccgt agttgcgcgt
    43861 gatacggacc tggacctcgt cggggatgac gctgccgcgc aggccggcca gcgccttctc
    43921 cacctcgcgg gccaccatca ccgcgttgct gcccttcttc ttggccacgg caatcgtcac
    43981 cgcgggccgg gattccgagt cggttgggcc cgagccgggc ccgaacccga tccggctgta
    44041 gctcgcgacc tcgtccgcgc cgtccacgac ctcggccacg tcgcgcaggt agaccggccg
    44101 gtcgtggtgc aggccgacca tcaggttgcg gacctcgtcc acgttctgga gaaacggtcc
    44161 gacctcgacg gcgatctcgc ggtcggcctt ggcgaacgag ccgctctcta tctgcgtgtt
    44221 ggagatcttc agcgcgccgg cgatctccat gggcgagagg ccgtaagcgg tcagccgctc
    44281 ggtatcgagg tacacgtgga ccacgcgctt gcggccgccg tggatcgtga tgcgcgcgct
    44341 gtccgggatg cgctggagct tgccggagac ctcctccgcg acacggtgga gcgcgccggc
    44401 atcgagcccc ggcccgtaga gcgcggcggt gacgatcggc acgtcgtcga tctcgatggg
    44461 tttgatcacc cagcccgcga tacccggcgt ggtcttgtca atattctgga agaccttgtt
    44521 gtagatcttg atcaggcttt cctcgcggtc ctcgccgacg aagaagcgca ccgtcaccac
    44581 ggccatgccc ggccgggaca tggagtagac gtattccacg ccgtcgatct gatagagcag
    44641 cttctccagg cgcgaggcga ccagttgttc gacctcctcc gcgctgccgc cgggatacat
    44701 caccatcacg tcggccagcg gcacgacgat ctggggctcc tcctcgcggg gcgtgaccag
    44761 cagggccacg gcgccggcgg cgaggctgat caggatcagc aggaccgcca ggttgccctt
    44821 gagaaaggcc tggatgaccg atgaaaagaa tccctgccgc tgttccatgg ctcactcctc
    44881 ccgccggggc tcggccagga ccgactcgcc cggcttcagc ccggatagaa tctccacgtc
    44941 gtctccatac tcgggcccgg tcttcaccag ccgccggatc gcccggccgt cgcggacgac
    45001 ctgcgcgaat tccaactggc ccgagcggta caccgcgctc ttcgggatcc ggatcgcctc
    45061 gcgcgcctcc tcttcgaccc agagccgccc aaacgtgccg ggcagcacct cgcccgccac
    45121 accctcgagc cggaccttga ccttctgcgt ccggctggac gggtccacct cgctgacgat
    45181 ctccgcgacc cggcccttga ccgtgcgcgc ggggcgctca agcgcgacgt ccacctcctg
    45241 gccgagcgcc agccggtcga tcagccggac gggcaccggc gcctcgaggc gcatccgcgc
    45301 ggggtcgtag acggcgagca acggcatgcc ggggttggcc aggtcgccgg cctcaatccg
    45361 gcgatcggtc acgatgccgt ggataggggc ggtaatctgc gcgtaggtca gcatgacttt
    45421 cacctggtcc acttgggcgc gggcggactg gaacatggat tccgccgcgg tcagcgcctg
    45481 ctgggtcgtc gcgtttttct cgaacagtcc gcgggcgcgc tcgtattccg actcggcgcg
    45541 ccgcaactgg gcctccgcgg ccgcgagctg ctcgcggatc tcgcggtcgt cgagcgtcac
    45601 gagcacctgc ccgttcgtca ccacatcgcc agcgcgggcg aagacggcct tgacgtaggc
    45661 cgggatgcgc gcgctcaaat tgattttctc atcggacgcc gtcgtgccga ccacgtggat
    45721 ccgggtcgcc aacggctccg ccttcacctc gaccgtcgcc gcgccttcgg ggacggcgat
    45781 tcccgccggg gcctcgacga cgccgggcgg aaccttctcc gcgcaggaac cggcggacca
    45841 cacgatcacg acggccagcc cgaccacgcc cgcaacgggg cgccaggcgc ttttaatcag
    45901 cgaagcgagc ttgctcatgt tcagttctcc tttggtccat tgaaataccg ccccaccagc
    45961 ttccccatcg cccggtcgag gttcgaccgc gcggtgacgt atcgtattcc gccgccaccg
    46021 cgcgggtgcg catggccgtg acgccgacct gggcctggag caggagcgcg acgtccgccg
    46081 cgccctgctc gtactgctcc cgcgtgatgc gcagcgcctc gcgcgcgctc tccaggctct
    46141 tgcgcgtgac ctccatgcgc tcgtgcgcct ccttcgcgcc caggtaggcc tgggtcaggt
    46201 cgagccgcag gttccgctcc gccttcgcct cctcggcccg cgccgcgtcg agctcggccc
    46261 gcgcggcggc gaccgccccg cgcgaacgg
//
LOCUS       DAOOTK010000014        45125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_618_length_45125_cov_6.467251, whole genome shotgun sequence.
ACCESSION   DAOOTK010000014 DAOOTK010000000
VERSION     DAOOTK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 45125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45125
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_45125_cov_6.467251"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2460)
                     /locus_tag="PK634_03130"
     CDS             complement(<1..2460)
                     /locus_tag="PK634_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOW96759.1"
                     /translation="MSEILAGQADYALLISGMAWLFAAVAARGLAREPASAGVWRWFT
                     WFGVAQGLSDWTDIFLLIRPEVPGLLIARLLLLLFASLVLVEFAAGSFWPAARGAFVR
                     HAAVLILITLAFLGGTAGLLGFLATLRPLLLLPAGLFAALALWRPGPVSAPAANRLPV
                     AAGGLAGYVLVLGLVLPAVPLGIACWLPTEPLFAAVLRVPPEFFRMTGAVLAGSALLG
                     HYSRVRRAASLAGAARRRHSPLLWLALSLAGVLSVGWVATETAGRSRAAAMRDEIRLR
                     TQIATASVPREPVLALRWGDADLESAGYQALKALMMSLRAANADLRFVSLMGVRGGES
                     YVLVDSEPPDSPDYSPPGQHYAEADPEYNRLLARAEDFVIGPLYDRWGTWMTGAAPVA
                     QPDPATVVNLALDIDAGGWAAQIALARLPMLLLVLLISGLVAVFLSVQGRTREEARVK
                     AALADRIRAQQEAVVHLARSATSASGDLAASAREITEQGSAALKTERVSVWLGRPGKG
                     LLRCVDLFERSTGRHSAGAVVQSATCPSYFAALAADRAIDAADASRDPRTAELTDDYL
                     APLRIASLLDAPIRVSGQVEGVVSFEQVGLPRTWQADEIRFAGELADHAAQALLAAER
                     ARAAAERKELEERMQQAQKLESLGILAGGIAHDFNNLLMPIMGHADMVSEELPSDSPH
                     QPNLKEIVGLTEKAAGLCRQMLAYAGRGHPSAEPQDLSQVVHHMQPMLEVAVARRAAV
                     ECHLAEALPPVNVDAAQFRQVVMNLVLNASEAVEARKNAEGRIRVTTDLVEADQGYLD
                     SCVPGSAALPGRYVFVEVDDNG"
     gene            complement(2468..6019)
                     /locus_tag="PK634_03135"
     CDS             complement(2468..6019)
                     /locus_tag="PK634_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOW96760.1"
                     /translation="MRKRTQSGAVVAMLFAACAAAAVEPPVLSGCEYDYPPYCVVKPD
                     GQADGFSVELLRAALAAMGREVTFKAGPWSELKQDLAEGRLQALPLVGRAPERESMFD
                     FTFPYLPVHGTILVRTDNADIRTTADLKGRSVAVLKGDNAEDYLRRSDFGATIVPLSS
                     FETALRELSAGQHDAVVIQKLVAFQLMRQAGITNLQAVGPPLKDFTQSLCFAVREGDA
                     ALLDTLNEGLSIVIADGTFRRLHAKWFAEIEAEGLLRSRIVVGGDNAYPPYEFLDENG
                     RPAGFNVDLTRAIARRMGLSVDIRLDTWATVRAGLESGELDVLQGMFYSAERTEKVSF
                     SPPHSIVHHAAAVRRGAPLPRGLPDLKGLALFVQNRDIMHDWAVAQGLGGQVQPVDSQ
                     ETALGRLHAGAGDAALVSVVQAQYWMKRHGWTGLDVSDVSLLSPEYCYGVRRGHEPLL
                     ALFSEGLAALKESGEYRQIQARWLGPYDASGVPLRRVLALVLVALALAAFFAVLGVLF
                     RRQVGRRTAELRASESLWRSYVESAPYGIYISDARGRLVQVNPEASQITGYNRDELTA
                     LELEDLVAPESRKAALRDFARLKESGEVRGEYAFVTKGGETRWWAVAGAKLTEDRYLG
                     YANDVTDRRAGEKLVRDAQAETQRLLEEARQARRALLSLVEDQRLGQEQLQALSERQD
                     ALLSAIPDIVMEVDANKVYTWANRAGLEFFGPDVVGREARYYFEGEQDTYRRVQPLFD
                     GKSDVIYLESLQRRHDGQKRLLAWWCRTLKDGQGRVTGAISTARDITDQKNLEDQFRQ
                     AQKIDAVGRLAGGVAHDFNNMLGVILGHAEMALEQIKPTQPVYEDLQEIQRAAQRSAD
                     LTRQLLAFARKQTVEPRVLNLNETVSGMLKMLRRLIGEDIELVWQPGANLWSVCIDPV
                     QVDQILANLAVNARDAIRGVGQLTICTENVALRSEDLATHPDAMAGEYILLKVGDNGA
                     GMDPVTQAHLFEPFFTTKGLGKGTGLGLATIYGVVKQNHGFIDVDSRPGRGTSFNIYL
                     PRALEDETTEPAKEGPSSVRGTETILFVEDSESLVKLVTTLLKKRGYNVLAAGLPSSA
                     LDMVRQYPKPIHLLIADVIMPGMNGRDMADRIRALRPDIRVIYMSGYTADVIAHHGVL
                     EPGVDFLQKPVSGKMLLAKIREVLDRKPGAPGTESTT"
     gene            complement(6016..8088)
                     /locus_tag="PK634_03140"
     CDS             complement(6016..8088)
                     /locus_tag="PK634_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW96761.1"
                     /translation="MNAHLAVLMLEAMVVYLLVLWAHSLRGRAGLGPFYALLGGITAV
                     MSWVTDAGVAVRAGGITFMVGSTVFYTAILLGVFVVYVFDGPPATRVAILTVAGVSAM
                     VPVIAAALHVQMRLLGAAPLGYVPLPSLRINAASVAATAADLVFLAVAWEFLGCRGPG
                     MKTWLRAFLSLLGVMGLDVLLFATGAFAGTPGYVGIMGGTLVSRFFIALFAFPFLYAY
                     LEWQSRGNGFALEPRPILAILKQVARARAAPPDPAQQEVTARLRMEETSIESEERYRA
                     FMDATDDLVFIKDDGFRYLFANEAYARFAGRPAASLRGCEDGDLMPPEAAARCRETDR
                     QALARNQVVISFEAIGDQYYETRKFPVSLKGGQRGIGGIIRDITERRKAEEHLRLQAQ
                     LLDSVREAVVASDLDGRITYWGKGAEELYGYKAAEVLGRPYRTFAGSIEPVEEEAFRR
                     AILDKGSWRGEHLQRRRDGAVFWTSTSISAVRNAEGVPCGFIGIDRDITEARTAEQAL
                     RTSEAQFSNALQIARAGHWEYDVARDTFIFNDNFYRIFRTTAAEAGGYTMRSADYARR
                     FCHPDDAALVGREIQAAIQTAEPNASRQIEHRMLYADGQVGYIAVRFFVVKDDLGRTI
                     RTYGVNQDITERKQAETRLREQMEELQRWHKITLGRELRILELKQEVNTLRAEAGKPA
                     KYDSAETE"
     gene            complement(8085..10325)
                     /locus_tag="PK634_03145"
     CDS             complement(8085..10325)
                     /locus_tag="PK634_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOW96762.1"
                     /translation="MSAERKRRMVGRFVAGLAGAGLLLAAGGWGAGVATRAADEDLRN
                     GLLEQAVAVARALDPALVRALSFTAADRDLPAFQRIRHYLRTYAKAAGLRSLYTMALR
                     DGRIVFGPESLEENDPFASPPGTVYEKPSAVDWAAFETAKPVSTGPLTDEYGTFVSAL
                     APVLEPHTGRVLLLVGLDVEARSWRARLARARRFPLATAGSLLGLLALGLALMEWRDR
                     GGLAGRAWLRHTETILIAALGLALTMAAVHLFRHEEDRAREARFRSLARAQTVGILEE
                     LRDLHNRVETLAAFFEASQHVTPEEFNAYVAPQVQSSLAERWLWLLSVPEGQAADAED
                     RVRREGVEDFRIWQPGGPGERVPASGRDHGYPVRYMAPSSNHPAALGYDLGSDPFYRP
                     AIEEARRSGLVSASDPAPLMSSSGRPPGFVVFRAVTAPQEGLVAAAVRLEELTAFPLR
                     RAMSPESPVVNGFFQLVPDRRPRCLSTSLPGHVAGLPGWFGEDSAYILRLPVFIFGNT
                     YAIVVHPGPGWAEKQRALGSGIVGVSGLLLTGLLTALFGLRARHSIALAEEVNARTGE
                     LRQKTAELETFFSSSLNLLCIADADGRFRRLNQEWERALGFSLSELEGRRFLDLVHPD
                     DVEATQQVVAAFGQGREVLNFVNRCRGRDGAYRWIEWRSIPSGRLIYAAARDITEQRR
                     TDQQLKEQLAELRRWQQAMLGREGRVAELKAEVNALLAQAGRALRYGGASAAPAGGGR
                     EREANA"
     gene            complement(10350..11549)
                     /locus_tag="PK634_03150"
     CDS             complement(10350..11549)
                     /locus_tag="PK634_03150"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOW96763.1"
                     /translation="MNLDIRTVFFGYVFSNALCVVVMALLWRHHRGHYDGIGRWLADF
                     FMQFIALGLVALRGQIPDWVSVVLANGLVVGGTMILYTGLERFLGRRSSSLRHYLVLA
                     IFLAAHAWFTWGQPSVHARNINLSLALPVVCGQCAWLMLTRRPGVRPSGAPVVGLVFA
                     AYSLFSLMRLVALAGGLRGSGDFGATLFDTLTILVYQMLFIALTLALFLLVIRRNRDE
                     LEAQGKALRESSALVHGLFDNMPSGVAMFSVRGDGATGDDYVVREINAAGLKLEGKRR
                     AEVIGRTLRELRPNIRPGGLRSVLRQVWRTEQPAFLPPTLYEDEQASRWYEAHIFCLP
                     TGELVCIYNDVTERQGAEHQLRENMEEIARMNKVMMNREERVLELKREVNRLLAEAGR
                     PPRFEAA"
     gene            complement(11998..13317)
                     /locus_tag="PK634_03155"
     CDS             complement(11998..13317)
                     /locus_tag="PK634_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOW96764.1"
                     /translation="MGKESILLVDDSETTRKVFTANLSRQGYEIQGAASLAEARDAVN
                     RNVFDGILLDLELPDGNGLDWLPELRESHPNAAIVIITGTGDIPSAVKAMQYGADHFL
                     AKPVNMDELIVFLRKGREFAELRRRNQFFSGVDRKRSVFYFGTNPAIGKMLELAETAA
                     RNDSSILLFGETGSGKGVLARWIHAHSARADKPFVDINCSSLKGDLLASEIFGHARGA
                     FTSAVESKQGLLEAADGSTLFLDEIGDMDPGVQASFLKVIEERQFRRVGEVKVRRSEF
                     RLICATNHDLQEQCAQGRFRNDLYFRINVFPIEVPPLRELREDIPGLIRHLLREMAGR
                     DLELAEEVMPLLTGYDWPGNIRELRNVLERAWLLAGGRALRAEHFPGLSAPRTPVGGT
                     LDLNRLEEQKIREALRRYGGDVERAAKALGLSRATLYRRIKPMESPE"
     gene            13472..14278
                     /locus_tag="PK634_03160"
     CDS             13472..14278
                     /locus_tag="PK634_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOW96765.1"
                     /translation="MLLPSSGKHLIATVMAALLAVAAFVFRHVAAPDSSRPQSAATAP
                     VARAAPAPSSPTTPSFATAPEDAGPRPDATARLEPPDEEARAAARRTLIETADLREAQ
                     QAAWEMANRLSLDNEDARTILRRLEATGLAPEEADLARTLIWALSLRPDQAPVERIAS
                     FALHHPDARVRRTALRTLGALDDPWAQATLERMALGDREPSVRREAVLFLSLRLPPGA
                     FAALARKALATEPEPSLQAAWNRALVFHRTSAETAAPPPPPGSAEKGSSL"
     gene            14275..15951
                     /locus_tag="PK634_03165"
     CDS             14275..15951
                     /locus_tag="PK634_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96766.1"
                     /translation="MISLRHAIVLTIALAAGLAPALEPLPEYNTGAVPKLKGYGNAPQ
                     EIYAAPPVVLDINTRAHESLDDSWSELTADANSQRDLFALLLATSLGAGLWENHPSSR
                     LEFQLGQLVSLSAVNLKDQHNVLTLDSHFDHSGIAKPILVKEGPGVMAISEFDIRLAR
                     SLLTTNAGMAIVDTPWGLIQMPMTNDWRIGAVSMAHELGHGIGLNHSYLGQYDDVLSA
                     SSVMAINRQYGQMQILAQGLCEDDVAALCRLYPRHAQRLSGATGSVIGRLVDSSGGQD
                     LFGGIATLVAPGERTLYHRISGFDTSLAAAKRGGFFCLDGVKPGTYELRVFPYTHFMA
                     QPIIHALALEEDGSTVSLGLPPEFFFAHDFKWAAVTQVIVKAGECLDLGFVKADEIRG
                     ADKPSLTLAGAQYDGAAKYSAYWFDFWRDGDTQFTHRSGSQPLPTYSARLPCGWYAVQ
                     MFGWNGSAWVTLQPWTWRNFNAKPVTLAWPKKTEPGPGGASMEAREYTAYVNAVTAAG
                     YGLLARVFPVDATSLKTCLPPGKYTLQVQAAFAKGTPLQTALPSTTFVVP"
     gene            16022..17638
                     /locus_tag="PK634_03170"
     CDS             16022..17638
                     /locus_tag="PK634_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOW96767.1"
                     /translation="MSAQTGKIRLWLGDEAVAQAAIDAGISGVYAYPGTPSTEITEYI
                     QKSAEARALGVRSAWSCNEKTAMEEALGMSFAGKRAMACMKHVGLNVAADPFMNAAIT
                     GAHGGLVVVVADDPSMHSSQNEQDSRYYGVFAQVPFFEPADQQEAYDQTREAFDFSER
                     WGVPVMLRLTTRLAHSRANVKRRVEQPRAQNPIRFHDDPRRFVLLPAIARRNYNELLK
                     KLPEFQAAAEASPYNTVEEGSDRSLGIVACGLAVNYLREAYGDEACPHPVLKISQYPA
                     PRKALARMLERCESVLVLEDGYPLVEDLMKGVNARPGAVRGRLDGTLPRAGELDPDIV
                     AQALGHAAPHTHEVPADVVPRPPSLCAGCPHANTYDFLRDAMADHPGGRVFSDIGCYT
                     LGALPPYEAINSCVDMGASVTMAKGAAEAGVHPSVAVIGDSTFMHSGLTGLLDIVEDK
                     TPMTLIIADNLTTGMTGGQPVAAAGRLEDIVKGLGVAPEHVHVLVPLPGNHKANTEIL
                     RKELDYRGPSVIISRRQCVVAARAGKSGIG"
     gene            17658..17942
                     /locus_tag="PK634_03175"
     CDS             17658..17942
                     /locus_tag="PK634_03175"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOW96768.1"
                     /translation="MTTFVMTGRYSAEAVKGISSGRTAKAGKIVQQCGGRIVEVYATM
                     GRNDLLVIAEFPGVGEAMKASVAFNKEFGIAFSTVPALPVKEFDKLVGRK"
     gene            17974..18570
                     /locus_tag="PK634_03180"
     CDS             17974..18570
                     /locus_tag="PK634_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOW96769.1"
                     /translation="MTHQDILIAGVGGQGILSIAGIIGTAAIERGLHVKQCEVHGMAQ
                     RGGAVVSHLRLSEKPIASDLIGRGRADLLLAMEPMEGLRHLPWLKHDGGVLVANRVPV
                     RNLKTYPATEALIAHIDRLQKRRLLDAEALARKAGTVRAVNSVLLGAASAFLMIPAEV
                     IRDAVAQFFDYKGATIVEQNLMAFDLGRAAAEENSKAS"
     gene            18660..19424
                     /locus_tag="PK634_03185"
     CDS             18660..19424
                     /locus_tag="PK634_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOW96770.1"
                     /translation="METISVIPCLDMKDGRVVKGVHFVALRDAADPVEAAQAYSAGGA
                     DEIAFLDITATVEGRRTMFDVVRKVAAVVKVPLTVGGGIKTCADIEEALACGASRVSI
                     SSAAFRNPRMVGEAVRQFGADRIVIAIDADVNDSLPSRREVYIDGGRTKTGRDAADFA
                     REMAALGAGRFLPTSKGADGTKDGYDLILTRAVAEATERPVIASGGAGKLDHFVDAVV
                     EGKASSVLAASVFHFGVFSIRQVKEHLSARGLSVNL"
     gene            19578..20279
                     /locus_tag="PK634_03190"
     CDS             19578..20279
                     /locus_tag="PK634_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOW96771.1"
                     /translation="MQLTVKQAAGILSVAEKTVYRWIQEQRLPAYRIEGQYRINRALL
                     FEWATAKKVNFSPALFQDGEPESRMPSLEQGLVNGGVFYRIEGTNPPSVLKAVVNLLR
                     LPDSTDRDFLFQAMLAREALQSTGIGDGIAVPHVRNPLIIEVDKPLVCLCFLEKPVDF
                     QALDGKPVFVLFTLVTPSVRAHLHLLSRLSFCLRDPTFKELLLRQASRDEILDAVRRI
                     EGLLSAPAPVQEPTL"
     gene            20276..22228
                     /locus_tag="PK634_03195"
     CDS             20276..22228
                     /locus_tag="PK634_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOW96772.1"
                     /translation="MMGSLWALALLLIPGALALLLPRLRWIGAAGAVAGAAVGLVACA
                     RLLGGAAAESLRVHWSIPGGLLYMELDALGAFFLLPVWIVTALAAVYGVEYLGRHPRA
                     PLAAALFNLLAASMVVVVLSRNALLFLAAWEIMALSSFGLVMLEDEKDSVRAAGWTYL
                     AASQLGTAALLLMFILLGSAAGSLDFDRFSAAGRGGWILALGLIGFGTKAGFIPLHTW
                     LPEAHPAAPSHVSAVMSGVMIKMGIYGLVRLLGLLGPIPAAAGWWFTAIGISSGLLGV
                     IYALAQHDLKRLLAYHSVENIGIIMLGLGVGLLGVRYGLPAMAAAGFAGGLFHVLNHA
                     LFKSLLFLGAGSVLHATGTRDLDRLGGLVKTMPVTAAAFLAGALAICGLPPFNGFASE
                     FLVYLGAFHGSTASAQGPVWGGLAVLVALSAIGGLALACFAKAFTVVFLGHAREARPQ
                     PARDPGPAMKAPMLLLASLCLAIGLGAPRLFPAFQYILAGRTDAAAQAAALGDARAAL
                     GTATGVMAVFVALALLAALWRIRRLAGRPVSQSVTWDCGYAAPAATMQYTGSSMAQPL
                     TEFLDPVLRAERHIEPPAGYFPASASFVSHTPDFFQRFLYRPLTALGARLLAPLRRVQ
                     HGNVHLYVLYIALTLLALLAWSLGAR"
     gene            22225..23136
                     /locus_tag="PK634_03200"
     CDS             22225..23136
                     /locus_tag="PK634_03200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009302919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOW96773.1"
                     /translation="MSVSGLIPVLAALTLSPLLFGVINKVKALVAGRQGPPVLQLYYD
                     LLKLSRKGAVYSATTSWLFRAGLVGGVAAVVAALLLVPLGGTPAVFAFRGDFILALYL
                     LGVARFFLVAAAMDTGSAFEGMGASREVFFSALAELALFVVLAALAHGTQTGTLSAML
                     GGVTPELWRAETPSLALLLIALVLVLLAENARIPVDDPNTHLELTMIHEVMVLDHGGP
                     DFALVLYTSALKLWLFAALVVGVLPIHDGPPAAAFAAGLAGMALVAALVGVVESAMAR
                     LRLARVPQLLLGAAALALVGFLLLAAG"
     gene            23141..23773
                     /locus_tag="PK634_03205"
     CDS             23141..23773
                     /locus_tag="PK634_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOW96774.1"
                     /translation="MTNTLLILILLTNLRLLISSRIHACVRWVAAQGILLGLLAVVGR
                     QADITLLGILFALFTAAIKGWVFPWLIFRAIRESEIRREMEPYVGYNLSLAIGLAALG
                     VSFWLGRRLPLPGLPGPLFVPVAFFSMFAGLFLIVSRKQAVTQALGYLVMENGIYLFG
                     VVAVRDEPLLVELGVLLDLLVAVFVMGITMFHIQRDVESLDANQLTQLRD"
     gene            23781..25220
                     /locus_tag="PK634_03210"
     CDS             23781..25220
                     /locus_tag="PK634_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOW96775.1"
                     /translation="MLLHALLLVPAAAGALAFVWRRPGAARFLLPAGAAAHAALTAAA
                     WLVRPASAHPNWLALDDTGLLFLSIASALFLAAAAYAPGYLRGRDRDARTMSEPLFAG
                     CALLFLASMTLVTLSRHFGLLWVAVEATTLASAPLIYYHRDARSLEATWKYLILCSVG
                     IALALLGTFSLSVAGEGAGLACLSVDELVRRAAGLNPTWLQAAALLLLVGYGTKMGLA
                     PMHTWLPDAHSEAPSMVSALLSGALLNCAFLGLLRVRQVVVAAGLQAFDAGAFLVLGV
                     LSVGLAWVFILRQADFKRLLAYSSVEHMGLLAIGIGLGGGGPYATLFHALNHSLVKAA
                     LFLLAGNLLTAYGSKAVEDVRGALRVAPRTARFWLVGLFAITGFPPFGVFVGKFLLLR
                     AAVQAGRIGLAGLLAVLLAMIFIAMSARMLPMVFGPAPGNRSPVRERWGAVLPSLVLL
                     LISLALGLWLPAPLADVLARAARTLGGAP"
     gene            25217..26734
                     /locus_tag="PK634_03215"
     CDS             25217..26734
                     /locus_tag="PK634_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOW96776.1"
                     /translation="MSGGLLLRNGEAVAATRLPVLPVPEWREAILDAVDGRARISSLF
                     GHREPDGRVRLTAVLARDAASTLEVLCADPGDVYPALTPECPQAHLFEREIAEQWAIR
                     PEGHPWFKPVRFHRPLRDVEPVLPECAAAEIQPCVTRFFRMDGDEIHEVAVGPVHAGI
                     IEPGHFRFQCHGETVFHLEISLGYQHRGVERALVGGPDARSIHYAETLAGDTTIGHAT
                     AYSQAIEALAACRVPARAHLLRALALELERLANHTGDLGALAADVGYLPTSAYCGRIR
                     GDFLNLTALLCGSRFGRRFVRPGGVTFDLEAARLQEMAGRFEQAMKDVTVAVNLLWET
                     NSVRARFEETGIVPEQAALEIGLVGPAARASGRERDARVDFPSGAYRFIQPPISTAPG
                     GDVQARAYVRWLEIQRSADFIRDVFKAMVPGEFRAAPGPLKPESFVVTMVEGWRGEIC
                     HAALTDERGRLRRYKVVDPSFHNWFGLALALRGQPISDFPLCNKSFNLSYCGHDL"
     gene            26748..27509
                     /locus_tag="PK634_03220"
     CDS             26748..27509
                     /locus_tag="PK634_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOW96777.1"
                     /translation="MLKIIEARIQQKQRTRPFPPADPRLSDRYQGRPVVDFSRCARDC
                     GRCVAVCPTGAIRKDAAGRMALDLGRCLFCGECAAACPADAIRFTGAYSMSARRKEDL
                     VFGGPWEGKAEALAAQVRRLFGRSLKLRQVSAGGCNACEADTNVLNTPFFDLGRFGIQ
                     FVASPRHADGLLITGPVPANMRTGLLKTWEAIPAPKLVIAVGACAISGGPYADHAEVQ
                     NGAAGLVPVDLFIPGCPPHPFTILDGLVRLLGRIR"
     gene            complement(27731..28846)
                     /locus_tag="PK634_03225"
     CDS             complement(27731..28846)
                     /locus_tag="PK634_03225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOW96778.1"
                     /translation="MSRWLRHLVWIVPLVIFDIWFFGHRRGGGCEPPVSVEEPAAILE
                     QEVAPETHPPWARLVYPTDQQGILQPGVPGVYQPTASGNPDSAMYGSVRTGSRGGRLV
                     PTFHEGLDIAALRRDAQGRPLDEVRAVADGVVAHVNRFGGNSNYGVYVVLRHDDPIGR
                     VFTLYAHLLSLAPGIQPGVVVKPGAVLGRMGNTSSSPIPMARGHLHFEIGLILNGQFG
                     TWFRAKKLKPDHGACHGWNLLGLNPLDFLRFQKEKPEASFLDFMATVPSAFDILLAVR
                     RKPDFFDRYPALWSGEQPGGAIVVACSENGVPLRGRAATAAERGQLGKLKSRIIRVHE
                     DVLGRNGSRLLTRRAGVWVLAVAGEQWREMLLYPSLP"
     gene            complement(28924..29070)
                     /locus_tag="PK634_03230"
                     /pseudo
     CDS             complement(28924..29070)
                     /locus_tag="PK634_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989029.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
     gene            complement(29137..30051)
                     /locus_tag="PK634_03235"
     CDS             complement(29137..30051)
                     /locus_tag="PK634_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOW96779.1"
                     /translation="MDPMIMLLAGGTMLVMAIVAGYTLGWANKVFHVAVDPRIEAINA
                     ALPGANCGGCGCVGCMSYAEAVAGGKIAPDKCTVGGSSCAQAIAKIMGVDLKPSWPYR
                     PVVHCRAHYAERLGRADYRGEKTCTGANLVGGVQGCTYGCLGFGDCVAACNYDAIHVV
                     DGLATVDYDKCIGCGACEKACPRHIISMTPFKSQRIIAVGCSNQDMGKDVKAVCKIGC
                     VGCMACSKASPIFKMTEGKIPRINYDEYDPENMDATLLAVKKCPVKNLIYVGKPSPEH
                     VEAVKDQEKPKIVQVDFKTTVDQAEWRG"
     gene            complement(30059..30799)
                     /locus_tag="PK634_03240"
     CDS             complement(30059..30799)
                     /locus_tag="PK634_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOW96780.1"
                     /translation="MSYIASLLVLAITTALINNMVFHFFVGNCPFIGVSRKLDMAFGM
                     GAAVTFVTTIAAALSWVATYFIMAPGAPLATWLYRAMTHDPTRVSDLSILSYIAYIFL
                     IAWAVQLVEMYVRKFFPALYKAFGVFLPLITTNCVILFVCLEIMKHIGAGAADPWGLD
                     KALVYAFFAGAGFLVAIVIMAGIREELDHCDVPKTLSGPGITLIIAGILSLAFMGLTG
                     VDSNLAKMIKKAPAAETSSVQDSGAQNP"
     gene            complement(30796..31458)
                     /gene="rsxE"
                     /locus_tag="PK634_03245"
     CDS             complement(30796..31458)
                     /gene="rsxE"
                     /locus_tag="PK634_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOW96781.1"
                     /translation="MSQDGPTAVERFLNGIIPENPTYRQILGICPTLAVTNTMESAMT
                     MAGTVLFVLVCSNVLTSLIRGLLKPHLRILVFTLTIAAFVTIADRFLAAYMYDMSKVL
                     GPYIPLVIVNCIIIARCEICAAKQGVWTAFMDAVGQSLGFMLGLMSIATIREILGFGT
                     WFGKWLGWRVIPVTAPEHWSTWVIMILPPGAFITLGLLMGVMNWIEAKHSARAKKATV
                     AA"
     gene            complement(31539..32093)
                     /locus_tag="PK634_03250"
     CDS             complement(31539..32093)
                     /locus_tag="PK634_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOW96782.1"
                     /translation="MKNSYIGQAWLVIVLSLVFGAALAGVQAALKPRIEQNKLNDTLG
                     QIPSLVPGATTGQAETIGGQTVYRALNDQGEQVGWMIPARGLGFADVLDLLVGLNKDL
                     SRVTGLSVLEQKETPGLGDRITEPRWRDQFKGKPTIPDLVVTKTAPKSDSEIQGVTGA
                     TISSVSVAGIVNGAVARLREGLRE"
     gene            complement(32090..33064)
                     /locus_tag="PK634_03255"
     CDS             complement(32090..33064)
                     /locus_tag="PK634_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOW96783.1"
                     /translation="MPSPAIHIAPAPHLFGGALTTQRMMFDVLLALVPVLVAALWQFR
                     LYAVIQLAICMVSAVAAEALFTAMRKKPILVRDGSALVTGAILAFSLPAKAPWYAGAV
                     GSFVAIGIGKVIFGGVGQNIFNPAMLGRAFAMIAFPAALGASAYVVPDSPIDGLTHAT
                     PLTALKMLGQSTGLLPLFLGATNGSIGETSALACLLGGAYLCLRRTASWEIPAGVIAA
                     TAVIGLLVQWFSPVAGWTVLHELCGGALLFGAFFIATDPVSSPLAPRGKFVFGLGVGA
                     LVMLIRKLSGYPEGVMFSVLIMNALVPLINRATIPTPVGGPVPAKKAA"
     gene            complement(33103..34458)
                     /gene="rsxC"
                     /locus_tag="PK634_03260"
     CDS             complement(33103..34458)
                     /gene="rsxC"
                     /locus_tag="PK634_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOW96784.1"
                     /translation="MIAAPSRAPGPGTFARGIHPPERKGFAAEVPIEIMPNPVQVAIP
                     LLQHTGAPCEPLVKVKDEVTLGQKIGEGKGFITAPVHASVAGAVGMPSVTTLPNGRHV
                     KTIPIKTGPNQLSADELKASLLGGDWPVSGLDAQSPEQIAQTAREAGLVGLGGAAFPT
                     HVKLTRNEKRPVDTLLLNGCECEPYLTSDYRLMIEFPRPIVTGALLAARATGATQLVI
                     AVEDNKPAAVESLRRAAEGTGVEVRVMETRYPMGGERQVIPAALDREVPTGGLPLDVG
                     VVVMNVGTSAALAAAVLRGKTLTHRVVSVTGAGIAQPKNVLAPIGSSYKDLIDFCGGL
                     KPDAARVIAGGPMMGFALNDLNITVTKGTSGITVLTHDDLKAVEETACVRCGRCVDVC
                     PLNLVPTRLALASRYQDWDMARRYHLMACCECGCCAYVCPAAIPLVQLMRMGKAQLPK
                     E"
     gene            complement(34455..34688)
                     /locus_tag="PK634_03265"
     CDS             complement(34455..34688)
                     /locus_tag="PK634_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96785.1"
                     /translation="MGGSLPPSAPDALAGGRMVLASASVFLLPLATAIAGALWIRSGP
                     SGQLTGALAGFAAGVAAASILTRFIRRHKKVVP"
     gene            34927..35361
                     /locus_tag="PK634_03270"
     CDS             34927..35361
                     /locus_tag="PK634_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW96786.1"
                     /translation="MDMKKISALVIDDNSEVLTLFKTLFFSEGIACDTAHNGHEGLEK
                     LGQNRYDIVFLDLIMPDVDGETLLGIIHRKFPGLHVVVMSVQDDDGVINEIMALGAKA
                     YLVKPSSTAAILDCVRNVERHKTADEVRADLLNARPQESAGK"
     gene            35384..35935
                     /gene="hpt"
                     /locus_tag="PK634_03275"
     CDS             35384..35935
                     /gene="hpt"
                     /locus_tag="PK634_03275"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOW96787.1"
                     /translation="MRPHHIRDVLFTDAEIARRLDTLVGALADECRAADFVMIGILRG
                     SFMFLADLVRLLYRHDVHPRIDFITLESYGSGTESSGQVRVTKDITLDVRGAHVLLVD
                     DILDTGRTLSFARQWIGEKGAAAVRTCTLLDKPSRRVVPCRADHVGFAVENHFVVGYG
                     LDYDGRYRELPYIARVTFLDEPG"
     gene            complement(35952..36608)
                     /locus_tag="PK634_03280"
     CDS             complement(35952..36608)
                     /locus_tag="PK634_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96788.1"
                     /translation="MSLLDGLKKLFGAKSGGAGRIYILDAAPLHGAARPGDRLSPRDQ
                     VQVLQQLSRFVEREKIQAQAVFEGRPLREVAEGENFGPVQVFFVENAAALPDRIVELF
                     RRAGAGRAVVVSAHPEVEKKVAEQGGSLIRPGTFRRAFDTGGGGGRPDGGGGGDSRMN
                     SRRRRRPRGGGRSDGGGRGRPDGGGNGGGDASQPPQPGSAPAQSQPKDPVRDLIDLVE
                     "
     gene            complement(36722..38428)
                     /locus_tag="PK634_03285"
     CDS             complement(36722..38428)
                     /locus_tag="PK634_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HOW96789.1"
                     /translation="MSRYWASETMARLRARFERLYGPAADRCLSRLAMLVGRYGIEEA
                     PSAPPWSEKDAWLITYADSIRTAGEKPLATLKRFLDRRLAGAISTVHVLPFFPSSSDD
                     GFSVVHYRTVDPALGDWPDVRALKERFNLAFDLVLNHVSRRSGWFLDYLAGVAPGRHF
                     FVEADPAADLSAVVRPRSTPLLHAARTRAGDRRVWTTFSEDQVDLDYSNPDVLFEILD
                     ILLFYIAMGARVIRLDAVAYLWKKPGTSCLHLPETHEIVRVMRDFLQLVSPQTILLTE
                     TNVPHEENVSYFGRGDESQMVYQFSLPPLLLHAFWRGDATALTRWAAALADPPPGCTF
                     LNFTASHDGIGLRPLHGLVPDGEVEWMEKETRDRGGQVSRRRNSDGSESAYELNISWY
                     SAMADPDPGRHAARFLCSQAVMMALKGIPAFYLHTLTSTPNDPDAVSRTGRARSINRH
                     AWNEAELASRLDDPASPASRLLRDLLALLQCRAACPAFHPDAAQAVLDLDPALFALER
                     AAPEGGQRVLALHNCSGRTIEASLPDGAAWEALPGFPARSHPEGGRLALRPYACEWLV
                     QA"
     gene            complement(38425..39699)
                     /locus_tag="PK634_03290"
     CDS             complement(38425..39699)
                     /locus_tag="PK634_03290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96790.1"
                     /translation="MTAEIENIGFISTRFAGTDGVTLEAYKWARVLEGEGHRCFWYAG
                     RLEAPPEASGCVPEAFFGHPENLWINERIWGKTARSPLVTRRIRAMSEYLKETLYDFL
                     RRFDVRIMVLENVLAIPMHVPLGLALSELMSETHLPAIGHHHDFYWERTRFSVNAVND
                     YLDMAFPSRDRELQHVVINQSAQEELARRKAVPSLLVPNVLDFENPPPPSDGYAADAR
                     VELGLAPDDILILQPTRIVPRKGIGHAISLVQALADKRCKLVISHEAGDEGTDYLETL
                     TTYAKKAGVDVRFFATRVADTRQVNGQGQKMYTLWDLYPHADFVTYPSFYEGFGNAFL
                     EAVYFKKPVLVNRYDNFARDIEPKGFRCPIMEGYLTREIVAEVRRILEDPAYRQDMVE
                     HNYQVALRHYSYAVLRRRLRTLIINIRGLANV"
     gene            39847..41025
                     /locus_tag="PK634_03295"
     CDS             39847..41025
                     /locus_tag="PK634_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HOW96791.1"
                     /translation="MSSMEQVLDILPAARKAADDLRDLLLANAVMIGEIPAATGNEQR
                     RVDFLLQRFAESGLSSCSSDERRNGAALLPGSDGRRHILLATGADTGVPDDADQTVEV
                     EPDRLVGPFVVDNSIALAVLATLPLLLERLQLRLRSHVLFLAAARSLGRGNLDGLKYF
                     LANARAPVHFGLCLESVQLGRLNHACLGMLRGEIVCRLPDDYNWAQHGATGSIIPMND
                     VITRINRIPVPRRPFTSIVLGSIHGGISHQNIARQTTLGFEVRSESADILHEIRQRME
                     DLVAEVGAQSGLRVDLDFFARREPGGLDIGHPLVRCGRAVLERLGVTPTTYRTTSGMS
                     AMVDRGIPALTVGVTTGERLGRLDEFDEACAIGPMAAGLAQLVGILLAMDGGHADGPA
                     "
     gene            41012..41989
                     /locus_tag="PK634_03300"
     CDS             41012..41989
                     /locus_tag="PK634_03300"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HOW96792.1"
                     /translation="MDPRDWLARNTFHHSAFQDLRRLVAEKERQGLRLSLCIPTLNEE
                     KTIGKEIMLLRAELVSRYPLFDEIAVIDSGSSDRTREVAAAFGADVYESADILPETGT
                     RRGKGENLWKAIYQLAGDIIVYLDADIKNIRPHFAYGLVGPLIERPEVRYVKAFYDRP
                     LAGAGGTRPSGGGRVTEILIRPLFSLFFPDLAALIQPLSGEYAVRRETLERLSFPIGY
                     GVETAHLMDVYRAHGLEAFAQTDLDRRVHRNQSTGDLGRMAFGILQTFLSRLRPGGGA
                     EALPELHAMLRQFQARDNAYELVEHHIVEEERPPMIDLPAYRQRRGLPP"
     gene            41986..42873
                     /locus_tag="PK634_03305"
     CDS             41986..42873
                     /locus_tag="PK634_03305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOW96793.1"
                     /translation="MTAAPQPDARIEWVRRLAARSAPMAPRPVGIEPRTPALRGIRAV
                     LFDVYGTLLISGAGDIGLSLDAVRADAMAGAFEAAGLGRLDDEPARRAAERFAALIRR
                     RHEERRAAGVDRPEVDIREIWRALLREIGAAPKEDQIGLLAIEYETRVNPAWPVPGAF
                     ELVETLAKRNLVLGLVSNAQFYTPLLIEALTGRAPEAWGFARDMCVWSFEEGAAKPSP
                     GLFRAAAERLRRSHGLEPRAALVVGNDAANDVAPAALLGFRTALFAGDARSYRPQPGH
                     APPDLLLTTLPQIASCLAD"
     gene            42953..43660
                     /locus_tag="PK634_03310"
     CDS             42953..43660
                     /locus_tag="PK634_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOW96794.1"
                     /translation="MSELKTYQIRCPRCRQEQPAGLYESINVKTDPDLRQKLMANAIN
                     VVRCEGCELSFRVDKPLLYHDPSRRIMIYLVPMADDAVENSERLFAQLLMNLENVLPA
                     EVRPPTIGLVFNRTELVERIFLLEAGLNERIIEYLKYLLFTRNADRLNPARKELLFNA
                     EDSTAESLCFVVQDVATRKLEAMLKYDRGVYATLAEMFDRDEQTPRLLELFPGPRISA
                     RALLLKELGREPASPES"
     gene            complement(43620..44426)
                     /locus_tag="PK634_03315"
     CDS             complement(43620..44426)
                     /locus_tag="PK634_03315"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96795.1"
                     /translation="MAPPGPFPCPSAAPAPRTDTDRLRALWREYGQPALIGLGLAAAF
                     LLGFGAYRSYRQNNVVKAAQLLARAGSVEQLQQVVSQYPSTPAAPMAMLTLAARQFDG
                     GQFELAQYTYAQFEQKYAKHPMKSAAELGRAQCLEAMGQMDQAEQAFDAFVAAHPDDY
                     LVPLARLNKARVLAQANKLEEARAAYEDFIAANPESGWLPQAENALDQINRQIRAGPA
                     AVPAPAPAPAVTIPEMSSIPLAPVAADSAAPAPEAPAVTIPETPAPAPAP"
     gene            44689..>45125
                     /locus_tag="PK634_03320"
     CDS             44689..>45125
                     /locus_tag="PK634_03320"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOW96796.1"
                     /translation="MRKMMKVLVPVFMLALVVAGCSKGPVEPQTNMPLTASYALPIDA
                     ALALATLHIYVVGATNHPVTAHRVTAAWDEMTVTWNSFGAAFDAAVVDGFMTDAGWQT
                     TDVTSLVQAWMNGTYANYGILLDEADDQTPRSIYASSESADFPT"
BASE COUNT         7110 a        15564 c        15121 g         7330 t
ORIGIN      
        1 gccgttatcg tccacctcga caaacacgta gcggccgggc aaggcggcgg agcccggcac
       61 gcaactatcc aggtagccct ggtcggcttc caccaggtcc gtggtcacgc gaatccggcc
      121 ctccgcgttc ttccgcgcct ccacggcttc ggatgcgttc aggaccaggt tcatcacgac
      181 ctgccggaac tgcgccgcgt ccacgttgac cggcggcagg gcctcggcca gatgacattc
      241 cacggctgcg cgccgggcca cggccacctc cagcatcggc tgcatatgat ggacgacctg
      301 ggacaggtcc tgtggctcgg cggacggatg cccgcggccc gcgtaggcca gcatctggcg
      361 gcaaaggccg gcggccttct cggtcaggcc cacgatttcc ttcaggttcg gctggtgcgg
      421 cgaatcggac ggaagctcct cgctcaccat gtcggcgtgg cccatgatcg gcatcaggag
      481 attattgaaa tcgtgcgcga tgccgccggc gaggatcccg agcgactcca gtttctgcgc
      541 ctgctgcatg cgctcttcca actccttgcg ctccgcggcg gcgcgggcgc gctcggcggc
      601 cagcagggcc tgggccgcgt ggtcggccag ttcgccggca aaccggatct cgtccgcctg
      661 ccaggtccgc ggcagaccga cctgctcgaa gctcaccacg ccctcgacct ggccggaaac
      721 ccggatgggg gcgtccagca gcgaggcgat gcgcagcggc gccaggtaat cgtccgtcag
      781 ctccgcggtg cgcgggtcgc gcgacgcgtc cgcggcgtca atggcgcggt cggccgcgag
      841 ggcggcgaag tagctcgggc aggtcgcgga ctgcacgacc gccccggccg aatgccggcc
      901 cgtggagcgc tcgaacaagt ccacgcagcg caggaggcct ttccccgggc gaccgagcca
      961 gacgctgact cgttccgtct tcaaggcggc cgagccttgt tccgtgatct cccgcgcgga
     1021 ggccgcgagg tcgcccgagg ccgacgtggc ggaacgggcg agatggacga cggcttcctg
     1081 ctgggcgcgg atgcggtccg cgagcgcggc cttgacgcgg gcctcctcgc gcgtccggcc
     1141 ctggacggac aggaagaccg cgaccaaccc gctgatcagc agcacaagga gcagcatcgg
     1201 caaacgcgcc agcgcgatct gcgcggccca gccgccggcg tcgatgtcca gcgccaggtt
     1261 cacgaccgtg gcggggtcgg gctgcgcgac cggggcggcg ccggtcatcc acgtgcccca
     1321 gcgatcgtac aggggaccga tgacgaagtc ctcggctcgg gccagaaggc ggttatactc
     1381 cgggtcggcc tccgcatagt gctggccggg cggcgagtag tccggcgaat cgggcggctc
     1441 cgaatccacc agcacgtagg attccccgcc gcgcacgccc atcagggaga cgaagcgaag
     1501 gtcggcgttc gcggcgcgca ggctcatcat gagcgccttg agcgcctggt agccggcgga
     1561 ctcgaggtcc gcgtcgcccc agcgcagggc aaggaccggt tcgcgcggca cggaggcggt
     1621 ggcgatctgc gtgcgcagcc gtatctcgtc ccgcatggcc gccgcgcggg agcgccccgc
     1681 ggtctcggtc gccacccagc cgacggacag gacgcccgcc agggagagcg ccagccacag
     1741 gagcggcgaa tggcgccggc gcgcggcgcc cgccagcgac gcggcgcggc gcacccggct
     1801 gtagtggccc agcagcgccg agccggccaa cacggcgccc gtcatacgga agaattccgg
     1861 aggcacacgc agcacggcgg caaacagcgg ctccgtcggc aaccagcagg cgatccccag
     1921 cggcacagcg ggaaggacca ggcccaggac caggacgtaa ccggccaggc ctccggcggc
     1981 caccggcagc cggttcgcgg ccggcgccga aaccgggccc ggccgccaca aggccagggc
     2041 ggcaaacagt cccgcgggaa ggagcagcag cgggcgcagc gtggccagga atcccagcag
     2101 cccggccgtg ccgcctaaaa aggccagggt gatcaagatg agaacggcgg cgtgccggac
     2161 gaacgcgccg cgggcggccg gccagaaact cccggcggcg aattccacga gcacgaggga
     2221 cgcgaacagc agaagcagga ggcgggcgat caacaggccc gggacctccg gccgaatcag
     2281 caggaagata tccgtccagt cggacaggcc ctgggccacg ccaaaccagg tgaaccagcg
     2341 ccagacgccg gccgacgccg gctcccgggc caggcccctg gccgccacgg ccgcgaagag
     2401 ccacgccatc ccgctgatca gcagggcgta gtccgcctgt ccggccagga tctcgctcat
     2461 gcttcgctca cgttgtcgat tccgtccccg gggcgccggg cttccggtcc agcacctccc
     2521 ggattttcgc cagcagcatt ttaccggaaa ccggtttctg gaggaagtcc acgccgggct
     2581 ccagcacgcc gtgatgggcg atcacgtcgg ccgtgtaacc ggacatgtat ataacccgga
     2641 tatccggccg cagcgcccgg atccggtccg ccatatcgcg gccgttcatc ccgggcatga
     2701 tcacgtcggc gatgagcagg tggatgggct tcgggtactg ccggaccatg tccagggcgg
     2761 aactcggcaa gccggcggcc agcacattgt agccccgctt cttcagcagc gtcgtcacca
     2821 gcttgaccag cgattcggag tcctcgacga acaggatggt ttccgttccc ctgacggagg
     2881 acgggccttc cttcgccggc tcggtcgtct cgtcctcgag ggcccgcggc aggtagatgt
     2941 tgaacgaggt gccccggccc ggccggctgt ccacgtcgat gaatccgtgg ttctgcttga
     3001 ccacgccata gatcgtcgcc aggcccaggc cggtgccctt gcccagcccc ttggtggtga
     3061 agaagggctc gaagagatgg gcctgcgtga cggggtccat tcccgcgccg ttgtcgccga
     3121 ccttgagcag gatgtactcc ccggccatgg cgtcgggatg ggtggccagg tcttcgctgc
     3181 gcagcgctac attctcggtg cagatggtga gctgccctac gccgcggatg gcgtcccgcg
     3241 cgttgaccgc caggttggcg aggatctggt cgacctggac gggatcgatg cacacggacc
     3301 acaggttggc gccgggctgc cagaccagtt cgatgtcctc gccgatcagc cggcggagca
     3361 tcttgagcat gccggagacc gtctcgttca ggttcaggac gcgcggctcg accgtctgct
     3421 tgcgcgcgaa ggcgagcagc tggcgggtga ggtcggcgga gcgctgcgcc gcccgctgga
     3481 tctcctggag gtcctcgtac acgggctgcg tgggcttgat ctgctcgagg gccatctccg
     3541 cgtggccgag gatgacgccg agcatgttgt tgaaatcgtg ggccacgccg ccggcgaggc
     3601 ggccgacggc gtcgatcttc tgcgcctggc ggaactggtc ttccaggttt ttctggtcgg
     3661 tgatgtcgcg ggcggtggag atcgcgccgg tcacgcggcc ctgcccgtcc ttgagcgtgc
     3721 ggcaccacca ggccagcagg cgcttctggc cgtcgtggcg tcgctgcagg ctctccaggt
     3781 aaatcacgtc gcttttaccg tcgaacagcg gctggacgcg ccggtaggtg tcctgttcgc
     3841 cctcgaagta atagcgggcc tcacggccga ccacgtcggg cccgaagaac tccaggccgg
     3901 cgcggttcgc ccacgtgtag accttgttcg cgtccacctc catcacgatg tccgggatgg
     3961 cggagagcag ggcgtcctgc cgttcggaga gggcctggag ctgttcctga cccagccgct
     4021 ggtcctcgac caggctcaac agggcgcgcc gcgcctgccg ggcctcctcc agcaggcgct
     4081 gggtttcggc ctgcgcatcc cggaccaatt tctcgccggc gcggcgatcg gtcacgtcgt
     4141 tcgcgtagcc gaggtagcgg tcttccgtca gcttggcgcc ggcgacggcc caccagcgtg
     4201 tctcaccgcc cttcgtcacg aaggcatact cgccccgcac ctcgcccgac tccttcagcc
     4261 gggcgaagtc ccggagggcc gccttgcgcg attcgggggc gacgaggtcc tccagctcga
     4321 gggccgtcaa ctcgtcccgg ttgtagcctg tgatctggga ggcctcggga ttgacctgca
     4381 cgagccggcc ccgggcgtcg ctgatataga tgccgtaggg ggcagactcc acgtagctgc
     4441 gccacaagga ttcgctcgcg cgcagttccg cggtccgccg tccaacctgc cggcggaaca
     4501 gcacgccgag cacggcgaag aacgccgcca gcgccagcgc gaccagcacc agggccagca
     4561 cccggcgcag cggcacgccc gaggcgtcat agggccccag ccagcgggcc tggatctgcc
     4621 ggtactcgcc cgattccttg agcgcggcca gcccctcgga aaacagggcg agcagcggct
     4681 cgtggccccg gcgcacgccg tagcagtact ccggcgagag cagggagaca tcggacacat
     4741 ccaggccggt ccacccgtgg cgcttcatcc agtactgggc ctggacgacc gacacgagcg
     4801 cggcatcgcc ggcgcccgcg tgcaggcggc cgagggcggt ctcctgggag tccacgggct
     4861 gcacctgccc gcccagcccc tgggccacgg cccagtcgtg catgatgtcc cggttctgca
     4921 cgaagagcgc aaggcccttc aaatccggca gcccccgcgg gagcggcgcg ccccggcgca
     4981 cggccgcggc gtggtggacg atcgagtgcg gcggcgagaa gctgaccttc tcggtgcgtt
     5041 cggcggagta aaacatgccc tgcaacacat ccagctcgcc gctttccagc cccgcccgca
     5101 ccgtggccca ggtatccagc cggatatcca cagacaggcc catgcgccgg gcgatggcgc
     5161 gcgtcaggtc cacgttgaac cccgcgggcc ggccgttctc gtccaggaac tcgtacgggg
     5221 gataggcgtt gtcgccgccg accacgatcc ggctgcggag gagcccctcc gcctcgatct
     5281 cggcgaacca tttcgcgtgc agccgccgga acgtgccgtc ggctatgacg atggatagcc
     5341 cctcgttcaa cgtgtccagc agcgccgcat ccccctcgcg cacggcgaag cacaggctct
     5401 gcgtgaagtc cttcagcggc ggcccgaccg cctgcaggtt ggtgatcccc gcctggcgca
     5461 tcaactggaa agccacgagt ttctggatga ccaccgcgtc gtgctgcccg gcggacagtt
     5521 cgcgcagagc ggtctcgaag gacgacaacg gcacgatcgt ggcgccgaag tcggaacgcc
     5581 gcaggtagtc ctccgcgttg tcgcctttca gcacggccac ggaccggccc ttgaggtcgg
     5641 cggtcgtccg aatgtcggcg ttgtcggtcc gcacgaggat cgtgccgtgg accgggaggt
     5701 acgggaacgt gaaatcgaac atcgattcgc gctccggcgc ccggcccacg agcggcagcg
     5761 cctggagacg cccctcggcc aggtcctgct tgagttcgga ccacggcccg gccttaaagg
     5821 tcacctcgcg gcccatggcc gcgagcgcgg cccggagcag ttccacggaa aacccgtcgg
     5881 cctggccgtc cggcttgacc acgcagtagg gaggatagtc gtactcgcag cccgagagga
     5941 ccggcggttc gacggccgcg gccgcgcagg ccgcgaacag catcgccacc accgcgccgg
     6001 attgagtccg cttcctcatt ccgtctccgc gctatcgtac ttcgccggct ttcccgcctc
     6061 ggcccgcagc gtgttgacct cttgcttcag ttccaggatc cgcagctccc ggcccagggt
     6121 gatcttgtgc cagcgctgca gttcctccat ctgctcgcga agccgggtct cggcctgctt
     6181 gcgctccgtg atgtcctggt tcacgccgta cgtgcggatc gtccggccca gatcgtcctt
     6241 gacgacgaag aatcgcacgg cgatgtaccc gacctgcccg tcggcgtaga gcatgcgatg
     6301 ctcgatctgg cgactggcat tcggctcggc ggtctggatg gccgcctgga tttcccggcc
     6361 caccagggcg gcatcatccg ggtggcaaaa gcggcgcgcg tagtccgcgg accgcattgt
     6421 atatccgcct gcctcggcgg ccgtggtccg gaaaatccgg tagaaattgt cgttgaagat
     6481 gaacgtgtcg cgggccacgt cgtactccca gtggccggcg cgcgcgatct gcaacgcgtt
     6541 ggaaaactgg gcctcgctcg tgcgcagcgc ctgctccgcc gtcctcgcct ccgtgatgtc
     6601 ccggtcaatg ccgatgaatc cgcacggcac gccctcggcg ttccggaccg cggagatcga
     6661 ggtggaagtc cagaacacgg cgccgtcccg ccgccgctgc agatgctcgc cccgccacga
     6721 gcccttgtcg aggatcgcgc gccggaaggc ctcctcctcc accggctcga tcgatcccgc
     6781 gaaggtccga tacggccgcc ccaggacctc cgcggccttg tacccgtaga gctcctcggc
     6841 gcctttgccc cagtacgtga tccgcccatc gaggtcggag gccaccacgg cctcgcgcac
     6901 gctgtcgagc aactgggcct ggaggcggag atgctcctcc gccttgcgac gctcggtgat
     6961 atcgcggatg atgccgccga tgccgcgctg cccgcccttg aggctcacgg ggaacttccg
     7021 ggtttcgtag tactggtcgc cgatggcttc gaagctgatc acgacctggt tccgggccag
     7081 cgcctgtcga tcggtctccc gacagcgggc ggcggcctcg ggcggcatca ggtccccgtc
     7141 ctcgcagccc cgcaggctgg ccgccgggcg cccggcaaac cgggcatagg cctcgttggc
     7201 gaacaggtag cggaatccgt cgtccttgat gaagaccagg tcgtccgtgg cgtccatgaa
     7261 ggctcggtag cgttcctcgc tctcgatcga cgtctcttcc atgcgcaaac gcgcggtgac
     7321 ttcctgctgc gcggggtccg gcggcgccgc gcgcgcccgg gccacctgct tgaggatggc
     7381 caggatcggg cggggctcca gggcgaagcc gtttccgcgg ctctgccact ccaggtaggc
     7441 gtaaaggaac gggaacgcga agagcgcgat gaagaaccgg ctgaccagcg tgccgcccat
     7501 gatgccgaca tagcccgggg ttcccgcgaa ggcgccggtg gcgaacagca ggacgtccag
     7561 ccccatcacg cccagcagcg acaggaaggc gcgcagccag gttttcatcc cgggtccgcg
     7621 gcagcccagg aattcccagg ccaccgcgag gaacaccagg tccgccgccg tcgccgccac
     7681 ggaggccgcg ttgatgcgga ggctcgggag aggcacgtaa ccgagcgggg ccgcgcccag
     7741 cagcctcatc tgcacgtgca gcgccgccgc gatgaccggg accatcgccg agacgccggc
     7801 gacggtcagg atcgcgacgc gcgtcgcggg cgggccgtcg aagacgtaca ccacgaacac
     7861 gccgagcagg atcgccgtgt agaacacggt ggagccgacc atgaaggtga tcccgccggc
     7921 ccgcaccgcc acgcccgcgt cggtcaccca cgacatcacc gccgtgatcc cgcccaacag
     7981 cgcgtagaac gggccgagcc ccgcccggcc gcgcagcgaa tgggcccaca ggaccagcag
     8041 gtagaccacc atcgcctcca gcatcagcac cgcaagatgg gcgttcacgc gttcgcctcc
     8101 cgttcccgcc cgccgccggc cggcgcggcc gacgcgccgc cataccgcag cgcccggccg
     8161 gcctgcgcca gcaacgcgtt gacctccgcc ttcagctccg cgacgcggcc ctcccggccc
     8221 agcatcgcct gctgccagcg gcgaagctcg gcgagttgct ccttcaactg ctggtcggtg
     8281 cgccgctgct cggttatatc gcgggccgcg gcatagatca accggcccga gggaatggat
     8341 cgccattcga tccagcggta ggccccgtcc cggccgcggc atcgattgac gaagttcagg
     8401 acttcccggc cctggccgaa ggcggccacg acctgctgcg tggcctcgac gtcgtcggga
     8461 tggaccaggt ccaggaagcg tcgaccctcc agttctgaca gggagaagcc cagcgctctc
     8521 tcccactcct ggttcaggcg ccggaaccgt ccgtccgcgt ccgcaatgca caggagattc
     8581 agcgagctgg agaagaaggt ttccaactcc gccgttttct gccggagctc tcccgtgcgg
     8641 gcgttgactt cttccgcaag cgcgatgcta tgccgcgcgc gcaggccgaa gagggcggtc
     8701 agcaggcccg tcagcagcaa cccggacacg ccgacgatcc cgctccccag ggcgcgctgc
     8761 ttctcggccc agcccgggcc cgggtgcacc acgatcgcat acgtgtttcc aaagatgaac
     8821 accggcagcc gcaggatata cgccgagtcc tcgccaaacc agcccggcag cccggccaca
     8881 tggcccggga gggacgtcga caggcaccgc gggcgccggt ccgggaccaa ctggaagaac
     8941 ccgttcacga cgggagactc cggactcatc gcgcggcgca gcggaaaggc ggtcagctcc
     9001 tccaaccgca cagcggcggc cacgaggccc tcctgcggcg ccgtcacggc gcgaaacacc
     9061 acgaaccccg gcgggcgccc ggacgaggac atcaacggcg cggggtccga ggccgagacc
     9121 aatcccgacc gccgggcttc ctctatcgcc gggcgataga agggatcgga ccccaggtca
     9181 taacccagcg cggccggatg attcgaggac ggcgccatgt agcggaccgg atagccatgg
     9241 tcgcggcccg aggcaggaac gcgttccccg ggcccgcccg gctgccagat gcgaaagtcc
     9301 tcaacgccct cgcggcgcac gcggtcttcc gcgtccgcgg cctgcccctc cggcaccgac
     9361 agcagccaga gccaccgctc ggcgaggctg gactggacct ggggcgccac gtaggcgttg
     9421 aattcctccg gtgtgacgtg ctggctggcc tcgaaaaagg cggccagcgt ctcgacgcgg
     9481 ttgtgaagat cgcgcagttc ttccaggatg cccaccgtct gcgcccgggc aagggacctg
     9541 aagcgggctt cgcgggcgcg gtcctcctcg tggcggaaca ggtgaacggc ggccatcgtc
     9601 agcgccaggc ccagggctgc gatcaggatc gtctccgtgt gccgcagcca ggcgcggccc
     9661 gccaggccgc cgcggtcccg ccattccatc agcgcgagcc caagcgccag caggccgagc
     9721 aggctgcccg ccgtagccag gggaaaacgc ctcgcccgcg ccaggcgggc gcgccagctc
     9781 cgcgcctcca cgtcgaggcc gaccagcagc agcacccggc cggtatgcgg ctcgaggacc
     9841 ggcgccaggg ccgacacgaa cgtgccgtac tcatcggtca gcgggccggt cgaaaccggc
     9901 ttggcggttt caaaagcggc ccagtccacg gcggagggtt tctcgtacac ggtccccggc
     9961 ggcgacgcga acggatcgtt ctcctccagg ctctccgggc cgaacacgat gcggccgtcg
    10021 cgcagggcca tggtgtagag gctgcgcagg cccgcggcct tcgcgtaggt gcgcaggtaa
    10081 tgccggatcc gctggaacgc ggggaggtcc cggtcggccg ccgtgaagga cagcgcgcga
    10141 acaagcgccg gatccagggc gcgcgcaacg gccacggcct gctccagcag cccgttgcgc
    10201 agatcctcgt ccgccgcgcg ggtcgccacc ccggcccccc atccgccggc ggccagaagc
    10261 agtcccgcgc ccgccaggcc cgcgacgaac cggccgacca tcctccgttt tcgttccgcg
    10321 ctcatgccgt gtcttctcgt cgcccgtccc tacgccgcct cgaaccgcgg cggccggccg
    10381 gcttccgcca gcagccggtt gacttccctc ttcaattcga gcacgcgctc ctcgcggttc
    10441 atcatcacct tgttcatgcg cgcgatctcc tccatgttct cccgcaactg gtgctccgcg
    10501 ccctgccgtt cggtcacgtc gttgtagatg cacaccagct cgccggtcgg caggcagaag
    10561 atgtgggcct cgtaccagcg ggaggcctgc tcgtcctcgt agagcgtggg cggcaggaag
    10621 gccggctgct cggtccgcca gacctgccgg agcaccgagc gcaggccacc gggccggatg
    10681 ttcggccgca gttcgcgcag cgtccggccg atgacttccg cccttctctt cccttccaat
    10741 ttcaggccgg cggcattgat ctcccggacc acgtaatcgt cgcccgtggc gccgtccccg
    10801 cgcaccgaga acatggccac gccgctgggc atgttgtcga acagcccgtg gaccagggcg
    10861 gaactctccc gcagggcttt cccctgggct tccagttcgt cgcggttgcg ccggatcacc
    10921 aggaggaaga gggccagcgt gagcgcgatg aacagcatct ggtagaccag gatcgtcagc
    10981 gtatcgaaca acgtggcccc gaaatcgccg ctcccgcgga gccctcccgc cagcgcgacc
    11041 aggcgcatca ggctgaacag gctgtaggcg gcgaagacca gcccgacgac cggcgcgccc
    11101 gacggccgga cccccggccg ccgcgtcagc atcagccacg cgcattggcc gcacacgacc
    11161 ggcagggcca gcgacaagtt gatgttccgc gcgtgcacgc tgggctgccc ccacgtgaac
    11221 caggcgtgcg cggccaggaa aatggccagc acaagataat ggcggagcga cgacgagcgc
    11281 cttccgagaa agcgctccag gcccgtgtac aggatcatcg tgccgcccac gaccaggccg
    11341 ttggcgagga ccaccgagac ccagtcggga atctgcccgc gcagggccac cagccccagg
    11401 gcgatgaact gcatgaagaa gtcggccagc cagcgaccga tcccgtcgta atgtccgcga
    11461 tggtggcgcc acaacagcgc catcacgacc acgcacagcg cgttgctgaa gacgtagccg
    11521 aaaaaaacag tccgaatgtc caagttcatg atgcctgatt tctccgatag gctgcacggt
    11581 ggaggctccc gacctcaagc cggcgcggta gacgctgcga caaacggaaa acgcggatgc
    11641 gggccggctt ttcgatctcc cgtgccaagg gtccgccgta gtggagggaa cagcctttta
    11701 tcgtggccgc tcgtctgttt cggtcggatt cgaaactacc ggcaccgcat ccgcgacata
    11761 aagcaactgt tgaccgattc gctttcatag caaaccgctt ggcacgttca gggtgtgagc
    11821 atgcagccgg catgccaaga acaggagctt ggcgtaatag ttatgatata aactggttgt
    11881 acatttttcc ggaaaccttc cgcgtctcat attgagacgg tgcggctcga aatgagacgg
    11941 gtgatgcagt ggatcgaccc cggcaagggg cgtgcgcgtc tccgggacga agccgcgcta
    12001 ctcgggagat tccatcggct tgatccggcg gtacagggtg gcccgtgaaa gcccgagggc
    12061 cttggcggcc cgctccacat cgccgccgta gcggcgaagc gcctcgcgga tcttctgctc
    12121 ttccagcctg ttcaggtcga gtgttccgcc caccggcgtg cgcggggcgg agaggccggg
    12181 aaagtgctcg gcgcgcaagg cccgcccccc ggccaggagc cacgcccgct ccagcacgtt
    12241 gcggagttcc cggatattgc ccggccaatc gtagccggtc agcaggggca tcacctcttc
    12301 cgccagttcc agatctcgcc ccgccatctc ccgaagcagg tggcggatca ggccgggaat
    12361 atcttcccgc agttcgcgaa gcggcggcac ttcgatcggg aacacgttga tccggaaata
    12421 caggtcgttc cggaagcggc cctgcgcgca ctgttcctgg agatcgtgat tggtcgcgca
    12481 gatcagccgg aactcgctgc ggcggacctt gacctcgccg accctccgga actgccgctc
    12541 ttcgatcacc ttcaggaacg aggcctgcac gccgggatcc atgtcgccga tctcgtcgag
    12601 gaacagcgtg ctgccgtccg ccgcttccag caggccctgc ttggactcga cggcggaggt
    12661 gaaggcgccc cgcgcgtggc cgaatatctc gctggccagc aggtcgccct tcaggctcga
    12721 gcagttgatg tccacgaaag gcttgtccgc gcgggcgctg tgcgcgtgaa tccaccgcgc
    12781 caggaccccc ttgcccgagc cggtctcgcc gaaaagcagg atggaggaat cattccgtgc
    12841 ggccgtctcc gccaactcca gcatcttccc gatcgccgga tttgttccaa aatagaacac
    12901 agaccgcttt cgatctactc cgctgaagaa ctggttcctg cgtcgcaact ccgcgaattc
    12961 tctacccttg cgcaggaaga caataagttc atccatgttg acgggcttgg ccagaaaatg
    13021 gtcggcgcca tactgcatgg ccttgacggc ggacgggatg tcgcccgtgc cggtgatgat
    13081 gacgatggcg gcgttcggat ggctttcccg caactccggc agccagtcca ggccgttgcc
    13141 gtccggcaat tccaggtcga gcaggatccc gtcgaacacg ttccggttca ccgcgtcgcg
    13201 cgcctcggcc agtgaagccg cgccctgtat ctcgtatccc tgccgcgaca ggtttgcggt
    13261 gaacaccttc cgcgtcgtct cggaatcgtc caccaggaga atggattcct tacccatggc
    13321 ttccggcctg ttacccggtc gctccggtcg caagctcatg ggaggagcta gctttttttt
    13381 cggcgcatgt caacggcgcg ccgaccgatt taacaagccg cgtccgaatc actattatat
    13441 taaacgttta atgcccacag aaacagggca catgcttctt ccttccagcg gcaagcatct
    13501 gatcgccacg gtcatggccg cccttctggc cgttgccgcg ttcgtcttcc gacacgttgc
    13561 ggctccggac tcctcacgcc cacaatcggc cgccacggcg cctgtcgcca gggccgcgcc
    13621 ggcgccatct tcgccgacca ccccctcctt cgcgacggcg ccggaggacg caggcccccg
    13681 gcccgacgcc acggcccggc ttgaaccgcc ggacgaagag gcccgggcgg cagcccgccg
    13741 gacgctgatc gagacggcgg acctgcggga ggcgcagcaa gccgcctggg aaatggccaa
    13801 ccggctgtcc ctcgacaacg aggatgcccg gacgatcctg cgaaggctgg aggcgacagg
    13861 cctggctccg gaggaagccg acctggcccg gaccctgatc tgggccttgt cattgcgacc
    13921 ggaccaggcg ccggtggaac gcatcgcttc ctttgccctg catcacccgg acgcccgcgt
    13981 gcggcgcacg gcgttgcgca cgctgggcgc cctcgacgac ccctgggcgc aggccaccct
    14041 cgaacgcatg gccctcgggg accgcgagcc ctcggtgcgg cgggaagccg tcctgttcct
    14101 gtcgctgcgc cttccgccgg gcgcgttcgc cgcgctggcc cggaaggccc tggccacgga
    14161 gcccgagccc tcgttgcagg cggcctggaa ccgcgcgctc gttttccacc ggacatctgc
    14221 cgaaaccgcc gcgccgccgc cccccccggg gagcgcggag aaaggaagct cgctatgatc
    14281 agcctgcgcc atgccatcgt cctgacgatc gccctcgccg ccggcctcgc cccggccctg
    14341 gaaccgctgc cggaatacaa caccggcgcc gtgccgaaac tcaaggggta cgggaacgcg
    14401 ccgcaggaaa tctacgcggc cccgcccgtc gtcctggata tcaacacgcg cgcgcatgaa
    14461 agccttgacg atagctggtc ggagctgacg gcggacgcga acagccagcg ggaccttttc
    14521 gccctgctgc tggccacgtc gctgggcgcg ggcctctggg agaaccatcc ctcctcccgc
    14581 ctggaattcc agctcggcca gctggtctcg ctttcggcgg tgaacctgaa ggaccagcat
    14641 aatgtgctga ccctggacag ccatttcgac cattcgggca tcgcgaaacc catactggtc
    14701 aaggagggtc ccggcgtcat ggcgatctcg gaattcgaca tccgcctggc gcgcagcctg
    14761 ctgaccacga atgccggcat ggcgatcgtg gacacgccgt gggggctgat ccagatgccc
    14821 atgaccaatg actggcgcat cggcgcggtg tcgatggcgc acgagctcgg gcacgggatc
    14881 gggctgaatc attcgtacct ggggcagtac gacgacgtgc tcagcgcctc ctcggtcatg
    14941 gcgatcaatc ggcagtacgg gcagatgcag atcctggccc aggggctgtg cgaggacgat
    15001 gtcgccgccc tgtgccgcct gtatccgcgg cacgcccagc ggctgtccgg cgccacgggc
    15061 tcggtcatcg ggcggctggt ggattcgtcc ggcggccagg atctgttcgg cggcatcgcg
    15121 acgctggtcg cgcccggcga gcgcacgctc taccaccgga tcagcggctt cgacacgtcc
    15181 ctggccgccg ccaagcgggg cggctttttc tgcctggacg gcgtgaagcc gggaacctac
    15241 gagctgcggg ttttccctta tacccacttc atggcgcagc cgattattca cgccctggct
    15301 ctcgaggaag acggttccac ggtgagcctc gggctgcccc cggagttctt cttcgcccac
    15361 gatttcaagt gggccgccgt aacgcaggtg atcgtgaagg cgggcgagtg cctggacctc
    15421 ggcttcgtga aggccgacga gatccgcggc gcggacaagc cgtccctgac gctggccggc
    15481 gcgcagtatg acggcgcggc gaagtactcg gcctactggt tcgacttctg gcgggacggg
    15541 gacacccagt tcacccaccg cagcggctcg caaccgttgc ccacctacag cgcgcggctg
    15601 ccgtgcggct ggtatgccgt gcagatgttc ggctggaatg gctcggcctg ggtgacgctt
    15661 cagccctgga cctggcggaa cttcaacgcc aagccggtga cgctggcctg gccgaagaag
    15721 acggagcccg gcccgggcgg cgcatccatg gaggcccgcg aatacacggc ctacgtgaac
    15781 gcggtgacgg ccgcgggcta cggactgctg gcccgcgtct ttccggtgga tgccacgtcg
    15841 ctgaaaacgt gcctccctcc ggggaaatac accctccagg tccaggctgc cttcgccaag
    15901 gggaccccgc tccagaccgc gctgccgtcc acgacgtttg tcgtgccgtg agcccggcgc
    15961 gcggatgaaa aaggcttgcg ccgggcgcgc catcggttgt taatagcccg tctccttgga
    16021 tatgagcgcg cagacaggca agattcgcct ctggctcggc gacgaggcgg tggcgcaggc
    16081 ggcgatcgac gccgggatct ccggcgtgta cgcctacccc ggcacgccgt cgaccgagat
    16141 cacggaatac atccagaaat ccgccgaggc ccgggcgctc ggcgtccgct ccgcctggag
    16201 ctgcaacgag aagacggcca tggaggaggc gctcggcatg tcgttcgccg ggaagcgcgc
    16261 gatggcctgc atgaagcacg tcgggctcaa cgtcgccgcc gacccgttca tgaacgcggc
    16321 gatcaccggc gcgcacggcg ggctggtcgt ggtcgtcgcg gacgatccct cgatgcactc
    16381 gagccagaac gagcaggaca gccggtacta cggcgtcttc gcccaggtcc cgttcttcga
    16441 gcccgccgac cagcaggaag cctacgacca gacccgcgag gccttcgact tctccgagcg
    16501 ctggggcgtg cccgtgatgc tccgcctgac cacgcgcctg gcccactcgc gcgcgaatgt
    16561 gaaacgccgc gtggagcagc cccgggccca gaatccgatc cggttccacg acgacccgcg
    16621 ccggttcgtc ctgctgcccg cgatcgcgcg gcgcaattac aacgagctgc tgaagaaact
    16681 gcccgagttc caggccgcgg ccgaggcttc gccgtacaac acggtcgaag aaggctccga
    16741 ccgctccctc ggcatcgtcg cctgcggcct cgcggtcaac tatctgcggg aggcgtacgg
    16801 cgacgaggcg tgcccgcatc cggtgctgaa aatctcgcag tacccggccc cgcgcaaggc
    16861 gctcgcgcgg atgctggagc gctgcgagtc ggtcctggtg ctcgaggacg gctacccgct
    16921 ggtcgaggac ctgatgaagg gcgtgaacgc ccggccgggc gcggtccgcg gccggttgga
    16981 cggcacgctc ccgcgcgcgg gcgagctcga ccccgacatc gtcgcgcagg cgctcgggca
    17041 cgcggcgccg cacacgcacg aggttccggc cgacgtcgtg ccgcgtccgc cgtcgctgtg
    17101 cgcgggctgc ccgcacgcga atacgtatga ttttctccgc gacgcgatgg ccgaccatcc
    17161 cggcgggcgc gtgttttcgg acatcggctg ctacacgctc ggcgcgctgc cgccgtacga
    17221 ggcaatcaac tcgtgcgtgg acatgggcgc gtcggtgacg atggccaagg gcgcggccga
    17281 ggcgggcgtg cacccctccg tggccgtgat cggcgactcg actttcatgc actcggggct
    17341 gaccggcctg ctggacatcg tggaggacaa gacgccgatg acgctgatca tcgccgacaa
    17401 cctgaccacg ggcatgaccg gcggacagcc cgtcgcggcc gcggggcggc tggaggacat
    17461 cgtcaaaggc ctcggcgttg cgcccgagca tgtccacgtc ctcgtgcccc tgccgggcaa
    17521 tcacaaggcg aacacggaga tcctgcgcaa agagctggat taccgcgggc cgtccgtcat
    17581 catttcccgg cggcagtgcg tggtcgcggc acgggccgga aaatcgggga tcggttaaca
    17641 aaaaaaggag atcgaagatg acaacgtttg ttatgacggg caggtactcg gcggaagcgg
    17701 tcaagggcat cagcagcggt cgcaccgcga aggccggcaa gatcgtccag caatgcgggg
    17761 gccggatcgt cgaggtctac gccacgatgg ggcggaacga cctgctggtg atcgcggagt
    17821 tccccggcgt gggcgaagcg atgaaggcct ccgtcgcctt caacaaggag ttcgggatcg
    17881 ccttctcgac cgtgccggcc ctgccggtga aggaattcga caagctggtc ggccgcaagt
    17941 agcctggacc tgcacgggcg ccgtccgttg gacatgaccc accaggacat actcattgcg
    18001 ggcgtaggcg gccagggcat cctgtccatt gccggcatca tcggtaccgc cgccatcgag
    18061 cgggggctgc acgtgaagca gtgcgaggtg cacggcatgg cccagcgcgg cggggcggtc
    18121 gtctcgcacc tgcggctctc ggagaaaccc atcgcctccg acctgatcgg gcgcggccgg
    18181 gcggacctgc tgctggcgat ggagccgatg gaagggctgc gccacctgcc gtggctgaaa
    18241 cacgacggcg gcgtgctcgt ggccaaccgc gtgccggtcc ggaacctcaa gacctacccg
    18301 gccacggagg ccttgatcgc gcacatcgac cggctgcaga agcggcgcct cctcgacgcc
    18361 gaggccctcg cgcggaaggc cggaacggtc cgtgcggtca acagcgtgct gctcggcgcg
    18421 gcctcggcgt tcctgatgat tcccgcggag gtgatccgcg acgccgtcgc gcagttcttc
    18481 gactataagg gcgcgaccat cgtggagcag aacctgatgg ctttcgattt gggtcgggcc
    18541 gccgcggaag agaattcaaa agcgtcatga aatgcgggct cgtgggaact cgcccctcca
    18601 ggcagaaaac aggaaatgga gggcggagct cccgcgacgc cggaaagaaa agagtcatca
    18661 tggaaacgat aagcgtgatc ccctgcctgg acatgaagga cggccgcgtg gtcaagggcg
    18721 tgcattttgt cgcgctccgc gacgcggccg accccgtgga ggcggcgcag gcctattccg
    18781 ccggcggcgc ggacgagatc gcgttcctcg acatcacggc caccgtcgag ggccgccgga
    18841 ccatgttcga cgtggtccgc aaggtggccg ccgtggtgaa agtgccgctg accgtgggcg
    18901 gcgggatcaa gacctgcgcg gacatcgaag aggcgctcgc ttgcggggcc tcgcgcgtga
    18961 gcatttcgag cgcggcattc cgaaatccgc gcatggtggg cgaggccgtg cggcagttcg
    19021 gcgcggaccg gatcgtgatc gcgatcgacg ccgacgtcaa cgacagcctg ccgtccaggc
    19081 gcgaggtcta catcgatggc ggccggacga agaccggacg ggacgcggcg gactttgcgc
    19141 gcgaaatggc cgccttgggg gccggccggt tcctgccgac cagcaaaggc gcggacggga
    19201 ccaaggacgg ctacgatctg atcctcacac gcgccgtggc ggaagcaacc gaaaggcccg
    19261 tcattgccag tggtggcgcc ggcaagttgg accattttgt tgatgccgta gtggagggca
    19321 aggcatcgtc cgtgctcgcg gcatccgtat tccattttgg agtattctca ataaggcagg
    19381 ttaaggagca tctctctgca cgaggccttt cggtaaacct gtagtcctgt tctcgaagca
    19441 gctcagcgat ggaatcagcc aattcttctg ttttctcacg gcttcaacat tattgttcta
    19501 atatggatca ttttgtatag ttcttattac tcattatgat ttgacattat tctcatgact
    19561 atgctttatc tccgctaatg caattgacag ttaaacaggc cgccggaatc ctctctgtcg
    19621 ccgaaaagac ggtctaccgc tggatacagg aacagcgcct gcccgcctat cggatcgagg
    19681 ggcagtaccg gataaaccgg gcccttctgt ttgagtgggc gacagccaag aaagtcaact
    19741 tctcgcccgc gctattccaa gacggggaac cggaatcgcg gatgccttcc ttggagcagg
    19801 ggttggtcaa tggaggtgtt ttctatcgga tcgaggggac gaatccgcca tccgtgctca
    19861 aagccgtggt gaatttgctg cggcttcccg actcgaccga tcgcgacttc ctgttccaag
    19921 ccatgctcgc ccgggaagcc cttcagtcca cggggatcgg cgacggtatc gcggtcccgc
    19981 atgtccggaa tccgctgatc atcgaagtgg acaagccgct cgtctgcctg tgcttcctgg
    20041 agaagccggt ggatttccag gccctggacg gtaaaccggt gttcgtgctg ttcaccctcg
    20101 tgacgccttc cgtccgcgcg catcttcacc tgctgtcccg cctctcgttc tgccttcgcg
    20161 atccgacgtt caaggaactg ctgctgcggc aggcgagccg ggacgaaatc ctggacgctg
    20221 tccgccggat cgaggggctt ctatcggccc ccgctcccgt gcaggagccg acactatgat
    20281 gggttcgctg tgggccctgg ctcttctcct tattccgggc gcgctggccc tgctgctgcc
    20341 ccgtttacgc tggatcggcg ccgccggcgc ggtggccggc gcagccgtgg gcctggtcgc
    20401 ctgcgcgcgc ctcctgggcg gcgccgccgc ggaatcgctg cgggttcatt ggtccatccc
    20461 cggcggcttg ctgtacatgg aattggacgc gttgggcgcg tttttccttc tgccggtctg
    20521 gatcgtcacg gcgctcgctg ccgtgtacgg ggtcgaatac ctgggccgcc acccgcgcgc
    20581 cccgctggcg gcggccctgt tcaacctgct ggccgcgagc atggtcgtcg tggtgctttc
    20641 gagaaacgcg ctgctgttcc tggcggcgtg ggaaatcatg gccttgagct ccttcggcct
    20701 ggtcatgctg gaggacgaga aggattccgt gcgggcggcg ggctggacgt acctggccgc
    20761 gtcgcagctt ggtacggccg cgctgctgct catgttcatc ctgctgggat cggccgccgg
    20821 ctctctggat ttcgatcggt tctcggccgc cggccgcggg ggctggatcc tcgcgctcgg
    20881 actgatcggg ttcggcacga aggcgggctt catccccctg cacacgtggc tgcccgaggc
    20941 gcacccggcc gcgcccagcc acgtgtccgc cgtgatgtcc ggcgtgatga tcaagatggg
    21001 catctacggg ctcgtccggc ttctcggtct gctgggcccc atcccggccg cggcgggctg
    21061 gtggttcacg gccatcggaa tctcctcggg cctgctcggg gtgatctacg cgctggccca
    21121 gcacgacctc aagcgcctgc tggcctacca tagcgtggag aacatcggca tcatcatgct
    21181 cggcctgggc gtcggcctgc tgggcgtgcg ctacggcctg cccgcgatgg cggccgcggg
    21241 gttcgccggc gggctgttcc acgtgctgaa ccacgcgctc ttcaaaagcc tgctcttcct
    21301 gggcgccggc tcggtcctgc acgcgacggg gacgcgcgac ctcgaccggc tcggcggcct
    21361 ggtcaaaacg atgccggtca ccgccgcggc ctttctcgcg ggcgcgctgg cgatctgcgg
    21421 cctgccgccg ttcaacgggt tcgccagcga attcctggtg tacctgggcg cgttccatgg
    21481 ctcgaccgcg tcggcccagg gcccggtatg gggcgggctg gcggtccttg tcgcgctgtc
    21541 cgccatcggc ggcctggccc tggcctgctt cgccaaggcg ttcacggtcg tattcctcgg
    21601 ccacgcccgc gaggcccggc cgcaacccgc gcgcgatccg gggccggcga tgaaagcgcc
    21661 catgctgctg ctggcgagcc tctgtctggc gatcggtctc ggcgcgcccc ggctgttccc
    21721 ggcttttcag tacatcctgg ccggacggac ggacgccgcc gcccaggccg ccgcgctcgg
    21781 cgatgcgcgc gcagccctcg gcacggccac gggcgtgatg gccgtgttcg tcgcgctcgc
    21841 gctgctggcc gcgctctggc gcatccgccg actggcgggc cggccggtgt cgcagtccgt
    21901 cacgtgggac tgcggctacg cggcgcccgc ggcgaccatg cagtacaccg gctcgtccat
    21961 ggcgcagccg ctgacggaat tcctggaccc cgtcctgcgt gccgagcgtc atatcgaacc
    22021 gccggccggc tacttccccg cgagcgcgtc cttcgtttcg cacacgccgg actttttcca
    22081 acgtttcctc taccggccgc tcaccgcgct cggcgcccgg ctgctggccc ccctgcggcg
    22141 cgtccagcac gggaacgtgc acctctatgt gctgtacatc gcgctgacgc tgctggcgct
    22201 gctcgcctgg agcctggggg cccgatgagc gtatccggcc tcatccccgt cctggccgcg
    22261 ctgacgctgt cgccgctgct gttcggcgtc atcaacaagg tcaaggccct cgtcgcgggg
    22321 cggcagggcc cgccggtact ccagctttac tacgatctgc tgaagctgtc ccggaaaggc
    22381 gcggtgtaca gcgccaccac gtcctggctg ttccgcgccg ggctcgtggg cggcgtcgcc
    22441 gcggtggtgg ccgctctcct gctggtcccg ctcgggggga cgcccgcggt cttcgcgttc
    22501 cgcggggatt tcatcctcgc gctctacctg ctgggcgtcg cgcgcttctt cctggtcgcg
    22561 gccgcgatgg acacgggctc cgcgttcgag ggcatgggcg cgagccgcga ggtcttcttc
    22621 tccgcgctgg ccgagttggc cctgttcgtc gtgctggccg cgctggccca cggtacgcag
    22681 accgggacgc tttcggccat gctcggcggc gtgaccccgg aactttggcg agccgagaca
    22741 ccctcgctgg cgctgctgct gatcgcgctg gtcctggtcc tgctggccga gaacgcccgt
    22801 atccccgtgg acgatcccaa cacgcacctg gagctgacga tgatccacga ggtgatggtc
    22861 ctcgatcacg gcggcccaga cttcgctctc gttctgtaca cctccgcgct gaagctctgg
    22921 ctgttcgccg cgctggtcgt cggcgtgctg ccgatccatg acgggccgcc ggccgcggct
    22981 ttcgcagcgg gcctcgccgg catggcgctg gtcgccgcgc tcgtcggcgt ggtcgagtcg
    23041 gccatggcgc ggctgcgtct ggcccgcgtc ccgcaactgc tgctcggggc ggccgcgctg
    23101 gcgctcgtgg gattcctgct gctggccgcc gggtgacgcc atgacgaaca ccctcctcat
    23161 tctcatcctg ctgacgaacc tgcggctgct gatctccagc cggatccacg cctgcgtccg
    23221 ctgggtcgcg gcgcagggca tcctgctggg cctgctcgcc gtggtcggac gccaggccga
    23281 tatcacgctg ctcggcatcc tcttcgcgct cttcaccgcc gcgatcaagg gctgggtctt
    23341 cccctggctg atcttccggg cgatccgcga gagcgaaatc cggcgcgaga tggagccgta
    23401 cgtcggctac aacctctccc tcgcgatcgg tctggccgcg ctcggcgtct cgttttggct
    23461 cggccggcgc ctgccgctgc ccggtctgcc gggtccgttg ttcgtgccgg tcgcgttctt
    23521 ctcgatgttc gccgggttat tcctgatcgt cagccgcaag caagcggtga cgcaggcgct
    23581 ggggtacctc gtgatggaaa acggcatcta cctcttcggt gtcgtcgccg tccgcgacga
    23641 gccgttgctg gtggaactcg gcgtgctgct ggacctgctg gtcgcggttt tcgtcatggg
    23701 catcacgatg ttccatatcc agcgcgatgt ggagagcctg gacgcgaacc agcttacgca
    23761 attgcgggat tgaaatggaa atgctgcttc atgccctgtt gctcgtcccc gccgcggcgg
    23821 gcgcgctggc tttcgtctgg cgccggcccg gcgccgcgcg cttcctgctg ccggcgggcg
    23881 ccgccgcaca cgcggcgctg acggcggcgg cctggctcgt ccgcccggcg tccgcccacc
    23941 cgaactggct ggccctggac gacacgggcc tgctgttcct ttccatcgcg agcgcgctgt
    24001 tcctcgccgc ggcggcctac gcgccgggct atctccgcgg gcgcgatcgc gacgcgcgca
    24061 cgatgagcga gccgctgttc gccggctgcg cgctgctgtt cctggccagc atgacgctcg
    24121 tgaccttgag ccgccacttc ggcctgctct gggtcgccgt cgaggccacg accctggcca
    24181 gcgccccgct gatctactac caccgcgacg cccgctcgct ggaagcgacg tggaagtacc
    24241 tgatcctctg ctcggtgggc atcgccctgg ccctgctcgg gacgttctcc ctttccgtcg
    24301 cgggggaggg ggccgggctc gcgtgcctgt cggtggacga actggtccgg cgcgcggccg
    24361 gcctgaaccc gacctggctt caagccgccg cgctgctcct gctggtcggg tacgggacca
    24421 agatgggcct ggcgcctatg catacgtggc tgccggacgc gcacagcgag gcgccctcga
    24481 tggtctccgc gctgctttcc ggcgcgctgc tgaactgcgc cttcctcggc ctgctgcgtg
    24541 tccgccaggt cgtcgtcgcg gccggcctgc aggccttcga tgccggagcg tttctcgttc
    24601 tgggcgtcct gtccgtcggg ttggcgtggg tcttcatcct gcgccaggcg gatttcaagc
    24661 ggctgctggc gtattcgagc gtcgagcaca tgggcctgct cgcgatcggg atcggcctcg
    24721 gcgggggcgg cccgtacgcg acgcttttcc acgcgctgaa tcactcgctg gtcaaggcgg
    24781 cgctgttcct gctggcgggc aacctgctga cggcctacgg gtcgaaggcc gtggaggatg
    24841 tgcgcggggc gctgcgcgtg gcgccgcgga cggcgcggtt ctggctggtg ggcctgttcg
    24901 cgatcaccgg ctttccgccg ttcggtgtgt tcgttggaaa gttcctgctc ctgcgcgccg
    24961 cggtccaggc cgggcggatc gggttggcgg gtctgctggc cgtcctgctg gcgatgatct
    25021 tcatcgcgat gtccgcgcgc atgctaccga tggtcttcgg gcctgcgccc gggaatcgaa
    25081 gcccggttcg cgagcgttgg ggcgcggtcc tgccgtccct ggtccttctg ctgatctcgc
    25141 tggcgctggg gctctggctg cccgcgccgc tggcggatgt tctggcgcga gccgcccgca
    25201 cgctgggagg cgcgccatga gcggcgggct gctcctgcgc aacggcgaag cggtcgccgc
    25261 gacccgcctg ccggtgcttc cggtgcccga gtggcgcgaa gccatcctcg acgccgtaga
    25321 cggccgcgct cggatcagtt cgctcttcgg tcaccgcgag ccggacggac gggtccgcct
    25381 gacggccgtc ctggcgcgcg atgccgcctc gacgctcgaa gtcctgtgcg cggatcccgg
    25441 cgacgtgtat ccggccctca cgcccgagtg cccgcaggcg cacctgttcg agcgcgaaat
    25501 cgccgaacag tgggcgatcc gcccggaggg ccatccgtgg ttcaagccgg tacggttcca
    25561 ccggccgctc cgcgacgttg agccggtgct tcccgagtgc gcggcggcgg aaatccagcc
    25621 gtgcgtgacg cggtttttcc gcatggacgg ggacgagatc cacgaggtgg ccgtcggccc
    25681 ggtccacgcg gggatcatcg agcccggcca cttccggttc cagtgccacg gcgaaacggt
    25741 gttccacctg gaaatttccc tggggtacca gcaccgcggg gtcgagcggg cgcttgtggg
    25801 cgggcccgat gcgcggtcca tccactacgc cgagacgctg gcgggggata cgacgatcgg
    25861 gcacgcgacg gcgtacagcc aggccatcga ggcgctggcg gcctgccgcg tccccgcgcg
    25921 ggcgcacctc ctgcgcgcgc tggccctgga gttggaacgg ctggccaatc ataccggcga
    25981 cctcggcgcg ctggccgcgg acgtcgggta tctcccgaca tcggcgtact gcggacgcat
    26041 ccgcggcgat ttcctgaatc tgaccgcgct gctgtgcggc agccggttcg gacgccgctt
    26101 cgtgcggccc ggcggtgtga cgttcgatct cgaagccgcg cgtctccagg aaatggcggg
    26161 gcgcttcgag caggcgatga aggacgttac cgtcgcggtc aacctgctgt gggagaccaa
    26221 ttccgtccgc gcgcggttcg aggaaaccgg cattgtgccg gagcaggcgg cattggaaat
    26281 cggtctcgtc ggccccgcgg cgcgggcctc gggccgggag cgcgatgccc gcgtggactt
    26341 cccctcagga gcctatcgct tcatccagcc gccgatctcg acggcgccgg gcggcgacgt
    26401 gcaggcccgc gcgtacgtgc ggtggctgga aatccagcgc tctgcggatt tcatacgcga
    26461 tgtattcaag gccatggtgc cgggcgagtt ccgcgccgca cccggaccgc tcaagccgga
    26521 gtccttcgtc gtcacgatgg tcgaaggctg gcgtggcgag atttgtcacg cggccctgac
    26581 ggatgagcgt gggcggttac gccggtacaa ggtcgtggat ccttcttttc ataactggtt
    26641 tggcctggcc cttgcgctgc gcggccagcc gatttccgat ttcccgctct gcaacaagag
    26701 cttcaatctt tcctactgcg ggcacgacct gtgatgggag cgacgccatg ctgaagatca
    26761 tcgaggcccg gatacagcag aagcaacgaa cgcggccttt cccgccggcg gatccgcggc
    26821 tgtctgatcg ttaccagggc cggcccgtgg tggatttctc gcgctgtgcg cgggattgcg
    26881 gtcgatgcgt ggcggtgtgt ccgaccggcg cgatacggaa ggacgcggcc gggcgcatgg
    26941 cgttggacct gggccggtgc ctgttctgcg gcgagtgcgc ggcagcctgc ccggcggatg
    27001 ccatccgatt caccggcgct tactccatgt ccgcccggcg caaagaagac ctcgtgttcg
    27061 gaggtccgtg ggagggaaag gccgaggccc ttgccgcgca ggtgcggcgg ctgttcgggc
    27121 gttcgcttaa actgcggcag gtcagcgcgg gcgggtgcaa cgcgtgcgag gcggacacga
    27181 acgtgctgaa cactccgttt ttcgatctcg gccggttcgg catccagttc gtcgcgtcgc
    27241 cccggcatgc ggacgggttg ttgatcaccg gaccggtgcc ggccaacatg cggaccgggc
    27301 tgctaaagac gtgggaagcg atacccgccc cgaagcttgt gatcgcggta ggggcctgtg
    27361 cgatttccgg cggcccctac gcagatcacg ccgaagtcca gaatggcgcc gccggcctgg
    27421 tcccggtgga cctgttcatc ccgggctgcc cgccgcatcc cttcacgata ctcgacgggc
    27481 tagtgcgcct gctgggtcgc attcggtaag cagaaccact tccacacttt gcaaagtgtg
    27541 gaagtgctta acgctgtatt atacttttat acagagagtt tcgcgaatcc tttgcgattc
    27601 atttcatact cacactttgc aaagtgtgaa aatacctgcg gagggcggtt tccacgcgtt
    27661 gcgccccctc cgaacagcgt aaaagggtga acggggaagg agccggagat tgagtatacc
    27721 cggtgatgct tcagggcaag gagggataca ggagcatttc ccgccattgc tcgccggcta
    27781 cggccagcac ccagaccccg gcgcggcgcg tgagcaggcg gctcccgttg cggcccagga
    27841 catcctcgtg gacccggatg atcctggact tcagtttgcc cagttgtccg cgctccgcgg
    27901 ccgtggccgc gcgcccgcgc aagggtaccc cgttttcgga gcaggccacc acgatggcgc
    27961 cgcccggctg ttcgccgctc cacaacgcgg gatagcgatc aaagaagtcg ggcttgcggc
    28021 ggaccgccaa caggatgtcg aacgccgacg gcacggtcgc catgaaatcg aggaaggagg
    28081 cctcgggctt ttccttctga aatcgaagga aatccagcgg attcagaccc agcaggttcc
    28141 agccatgaca cgcgccgtga tccggtttca gcttctttgc gcgaaaccag gtgccgaact
    28201 gcccgttcag tatcaggccg atctcaaaat ggagatgccc gcgagccatg ggaatgggcg
    28261 acgatgacgt gttgcccatc cgtcccagca cggcgcccgg cttgaccacc acgcccggct
    28321 ggataccggg cgccaagctc aacaggtgcg catagagggt gaagacccgg ccaatcggat
    28381 cgtcatggcg aagcacaaca taaacgccat agttggagtt cccaccaaac cggttgacgt
    28441 gcgcaacgac gccgtcggca acggcccgaa cctcgtcgag cggccggccc tgggcatcgc
    28501 gccggagcgc tgcaatatcc aacccttcgt ggaatgtcgg caccagccgc ccgcctcgcg
    28561 agcccgtccg gaccgacccg tacatcgccg aatcgggatt gccggacgct gtcggctggt
    28621 agactcccgg cacgcctggc tgcagaatgc cctgctgatc cgtcggatac acaagacgcg
    28681 cccagggcgg atgcgtttct ggcgccacct cctgctcgag gatcgcagcc ggctcttcca
    28741 cggaaaccgg cggctcgcac ccgccacccc tccggtgtcc gaaaaaccag atgtcgaaga
    28801 tcaccagcgg aacgatccag accaggtgcc tcagccagcg agacatccgt ctcgccgcgg
    28861 gggcctgcac aggacgattc cttaattcgt gcaggggtaa atgcatggga acaggcagat
    28921 ggcccgaccc gactcgtcgg gttcgccgag ttcgcaccgc tggcattcca ggcccgcgac
    28981 gcggccgtaa tcgtcgccga ggatgcggat cgtgttctgg aggaggtgaa actcgacgcc
    29041 ttcctccttc gcgcgccgga cctcctcggc caactgctgc tttttcagga tagtattcat
    29101 ccccgctgac ccatttcggg aacggccgcc gccggattat ccccgccact cggcctggtc
    29161 cacggtggtc ttgaagtcca cctggacgat cttcggcttt tcctggtcct tgaccgcctc
    29221 gacatgctcg gggctcggct tgccgacgta aatcaggttc ttgaccgggc acttcttcac
    29281 ggcgaggagc gtggcgtcca tgttctccgg gtcgtactcg tcgtaattga tgcgcgggat
    29341 cttgccctcg gtcatcttga agatcgggct ggccttggag caggccatgc agccgacgca
    29401 gccgatcttg cagacggcct tgacgtcctt gcccatatcc tggttcgagc agccgacggc
    29461 gatgatgcgc tgggacttga acggggtcat cgagatgatg tggcgcgggc aggccttctc
    29521 gcaggcgccg catccgatgc acttgtcgta gtcgacggtc gccaggccgt ccacgacgtg
    29581 gatcgcgtcg tagttgcagg ccgcgacgca gtcgccgaac ccgaggcagc cgtacgtgca
    29641 gccctgcacg ccgccgacca ggttggcgcc cgtgcaggtc ttctcgccgc ggtagtccgc
    29701 ccggccgagc cgctcggcgt agtgggcgcg gcagtggacc acggggcggt agggccagga
    29761 gggcttgagg tccacgccca tgatcttcgc gatggcctgg gcgcagctcg agccgccgac
    29821 cgtgcacttg tccggagcga tcttgccgcc cgcgacggcc tcggcgtagc tcatgcagcc
    29881 gacgcagccg cacccgccgc agttggcgcc cggcagcgcg gcgttgatcg cctcgatgcg
    29941 cgggtccacc gcgacgtgga acaccttgtt ggcccacccg agcgtgtagc cggcgacaat
    30001 cgccatcacc agcatcgtcc cgcccgccag aagcatgatc atcggatcca tgggaaaatc
    30061 aggggttctg ggctccggag tcctggacgc tggacgtttc cgccgccggc gccttcttga
    30121 tcatcttcgc caggttgctg tccacgccgg tcaggcccat gaaggccagc gagaggatgc
    30181 ccgcgatgat cagggtgatg ccgggcccgc tcaaggtctt cggcacgtcg cagtggtcca
    30241 gctcctcgcg gatgccggcc atgatgacga tggcgaccag gaatccggcg cccgcgaaga
    30301 acgcgtacac cagcgccttg tccaggcccc acgggtccgc ggcgcccgcg ccgatgtgct
    30361 tcatgatctc gaggcagacg aagaggatca cgcagttggt cgtgatcagc ggcaggaaca
    30421 cgccgaacgc cttgtagagc gccgggaaga acttgcgcac gtacatctcc accagctgca
    30481 ccgcccaggc gatcaggaag atgtaggcga tgtaactcag gatgctcagg tcgctgacgc
    30541 gggtggggtc atgcgtcatg gcccggtaga gccacgtcgc cagcggcgcg ccgggggcca
    30601 tgatgaagta ggtggccacc cagctcaacg ccgccgcgat ggtcgtgacg aacgtcaccg
    30661 ccgcgcccat gccgaaggcc atgtccagct tgcgcgagac gccgatgaac gggcagttcc
    30721 ccacgaagaa gtggaaaacc atgttgttga tcagcgcggt cgtgatcgcc aggacgagga
    30781 gggacgcgat gtagctcatg ccgccaccgt cgctttcttc gcccgcgcgc tgtgcttcgc
    30841 ctcgatccag ttcatgacgc ccatcaggag gccgagggtg atgaacgcgc ccggcgggag
    30901 gatcatgatc acccacgtcg accagtgctc gggggcggtc accgggatca cccgccagcc
    30961 cagccacttg ccgaaccacg tgccgaagcc caggatctcg cggatggtcg cgatgctcat
    31021 caggcccagc atgaatccga gcgactgccc gacggcgtcc atgaatgccg tccagacgcc
    31081 ctgcttggca gcgcagatct cgcagcgcgc gatgatgatg cagttgacga tgaccagcgg
    31141 aatgtacggc cccagcacct tgctcatgtc gtacatgtaa gcggccagga accggtccgc
    31201 aatcgtgacg aaggccgcga tggtcagcgt gaagaccagg atacgcaggt ggggcttgag
    31261 caagccgcgg atcaagctcg tcagcacgtt ggagcagacc agcacgaaca agacggtgcc
    31321 cgccatggtc atggcgcttt ccatcgtgtt ggtcaccgcc agcgtcgggc agatgccgag
    31381 gatctggcgg tacgtcgggt tctccgggat gatcccgttg agaaaccgct ccaccgctgt
    31441 cggcccgtct tgactcatgg tcctttcatc ctgctgctca tcgttcgggc cacgcgctta
    31501 cgcgcgcggc tacgtagccg ccgccgtaag gcggtggcct attccctcaa tccttcccgg
    31561 aggcgcgcca cggcgccgtt cacgatgccc gccacgctca ccgaggaaat cgtcgcgccg
    31621 gtgacgccct ggatttcgct gtccgacttc ggcgccgtct tggtcaccac caggtcgggg
    31681 atcgtgggct tgcccttgaa ctgatcgcgc caccggggct ccgtgatgcg gtcgcccagg
    31741 cccggcgttt ccttctgctc cagcacggaa agtcccgtga cgcgcgagag gtccttgttc
    31801 aggcccacca gcaggtcgag gacgtccgcg aagcccaggc cgcgcgcggg aatcatccag
    31861 ccgacctgtt cgccctgatc gttcagcgcg cggtatacgg tctgaccgcc gatcgtctcc
    31921 gcctggccgg tcgtggcccc cggcacgagc gacgggatct ggcccaacgt gtcgttgagc
    31981 ttgttctgct cgatcctcgg cttcagcgcc gcctgcacgc ccgccagcgc cgcgccgaac
    32041 acgagcgaca gcacgatcac cagccaggcc tggcctatgt acgagttctt catgccgcct
    32101 tcttcgccgg gaccggcccc ccgaccggcg tcgggatcgt cgcgcggtta atcagcggga
    32161 ccagcgcgtt catgatcagc accgagaaca tcacgccctc gggatagccg ctcaacttgc
    32221 ggatcagcat caccagcgcg cccacgccga gcccgaagac gaacttgccg cgcggcgcga
    32281 gcgggctgga gaccgggtcc gtcgcaatga agaacgcgcc gaacagcagc gcgccgccgc
    32341 acagctcgtg gaggaccgtc cacccggcga ccgggctgaa ccactggacc agcaagccga
    32401 tgaccgccgt ggccgcgatc acgccggcgg ggatctccca cgaggccgtc cgtcgcaggc
    32461 acaggtaggc gccgccgagc aggcaggcca gggcgctcgt ttcgccgatc gaaccgttgg
    32521 tcgcgccgag gaacaggggc agcaggccgg tgctctggcc cagcatcttc agcgccgtca
    32581 gcggcgtcgc gtgcgtcagg ccgtcgatcg gggagtccgg caccacgtag gcggaggccc
    32641 ccagcgcggc cgggaaggcg atcatcgcga acgcgcgccc gagcattgcc gggttgaaga
    32701 tgttctggcc gacgccgccg aagatcacct tgccgatgcc gatcgcgacg aacgacccga
    32761 ccgcgccggc ataccagggc gccttcgccg ggagcgagaa ggcgaggatc gcgccggtga
    32821 ccagcgccga cccgtcgcgg accaggatcg gtttcttccg catcgcggtg aagagcgcct
    32881 cggcggcgac cgcgctgacc atgcagatcg ccaactggat caccgcgtag agccggaact
    32941 gccacagggc ggccacgagg acaggcacca gcgccagcag cacgtcgaac atcatccgct
    33001 gcgtcgtcag cgccccgccg aacaggtgcg gggccggggc gatatggatc gccggcgacg
    33061 gcatgacagc cggtgcttcc gttgttgacg tggccgtatc cattattcct tcggcaattg
    33121 ggctttaccc atgcgcatca attggaccag cgggatcgcc gccggacaga cataggcgca
    33181 gcagccgcac tcgcagcagg ccatcaggtg gtagcgccgg gccatatccc agtcctggta
    33241 gcgcgaggcc agggccaggc gcgtggggac caggttcagc gggcagacgt ccacgcaacg
    33301 cccgcagcgg acgcaggcgg tctcctcgac ggccttcagg tcgtcgtggg tcaggaccgt
    33361 gatgccgctg gtgcccttgg tgacggtgat gttcagatcg ttcagggcga agcccatcat
    33421 cgggcctccc gcgatcacgc gggccgcgtc gggtttgagc ccgccgcaga aatcgatcag
    33481 gtccttgtag ctcgagccga tcggcgccag gacgtttttc ggctgcgcga tgcccgcgcc
    33541 ggtgacgctg accacgcggt gcgtcagcgt tttcccgcgc agcacggccg cggcgagcgc
    33601 ggcggaggtc ccgacgttca tgaccacgac gccgacatcc agcggcaggc cgccggtcgg
    33661 tacttcgcga tccagcgcgg ccgggatcac ctgccgctcg ccgcccatcg ggtagcgcgt
    33721 ctccatcacg cggacctcga cgcccgtgcc ctcggcggcc ctgcgcagag actcgacggc
    33781 cgccggcttg ttatcctcaa cggcgataac cagttgtgtt gctcccgtgg cgcgggcggc
    33841 cagcagcgcg ccggtgacga tcgggcgcgg gaactcgatc atcaaacggt agtcggaagt
    33901 caggtagggc tcgcactcgc agccgttcag tagcagcgtg tccacgggcc gcttctcgtt
    33961 gcgcgtcagc ttgacgtgcg tcgggaaggc cgcgccgcca aggcccacga ggcccgcctc
    34021 gcgcgccgtc tgtgcgatct gctccggcga ctgcgcatcc aggccggaca ccggccagtc
    34081 gccgccgagc agggaggcct tcaattcgtc cgcggaaagc tggttcgggc ccgtcttgat
    34141 cgggatcgtc ttcacgtgcc gcccgtttgg gagcgtcgtg acggacggca tgccgacggc
    34201 gccggcgacg gaggcatgga ccggcgcggt gatgaagccc tttccctcgc cgatcttctg
    34261 gcccagcgtc acctcgtcct tgaccttcac cagcggctcg cagggcgcgc cggtatgctg
    34321 gagaaggggg atggccacct gcaccggatt cggcataatt tcgatgggca cttcggccgc
    34381 aaagcctttt cgttcagggg gatggatgcc ccgcgcgaag gtgcctgggc ccggggctct
    34441 ggaaggcgcg gcgatcacgg cacaaccttc ttatgtcggc ggatgaagcg agtgagaatg
    34501 gacgcggcgg ctacgccggc ggcgaagccg gccagcgccc cggtcaattg gccggacggc
    34561 ccgctgcgga tccagagcgc gcccgcgatc gcggtggcca ggggcagcag gaataccgaa
    34621 gcggaagcca gcaccatgcg gccgccggcc agcgcgtcgg gcgcgctcgg cggaaggctg
    34681 ccgcccatgg agcactgctc gcaggctccg gagcatgatt ccggcctgtt ggtctggacc
    34741 tggttcatcg gggccaatat gtctagcgtt ctatgctcgg gatacaagaa aaaaaacagc
    34801 cgggcgaggc ccgttgcttt ttttgtagga aaaccggaag ggcatgtttt atgattgtgc
    34861 ccgtcgcagc gcgccggtag ttagtcacag ggataagccc agttccggcg aatcggcggg
    34921 ccgttcatgg acatgaagaa aatatcggca ctggtcatcg acgacaacag cgaggtgctc
    34981 acgctgttca agaccctttt cttctccgag ggcatcgcct gcgacacggc gcacaacggc
    35041 catgaaggcc tggagaagct ggggcagaac cgctacgaca tcgtgttcct agacctgatc
    35101 atgccggatg tggacggcga gaccctgctg ggcatcattc accggaagtt ccccggactc
    35161 cacgtggtgg tcatgtccgt gcaggacgac gacggcgtga tcaacgagat catggccctg
    35221 ggcgcgaagg cctacctcgt caagccgtca tccaccgccg ccatcctgga ctgcgtgcgc
    35281 aacgtcgagc ggcataagac cgccgacgaa gtccgcgccg acctcctgaa cgcccggccc
    35341 caggagtccg ccggaaaata gccggcgcct ctttttttta cccatgcggc cgcaccatat
    35401 tcgcgacgtc ctgttcaccg acgcggagat cgcccgccgg ctggacacgc tcgtgggcgc
    35461 cctggccgac gaatgccgcg cggcggactt cgtgatgatc ggcatcctgc gcggcagctt
    35521 catgttcctc gccgacctcg tgcgcctgct gtaccggcac gatgtccacc cgcgcatcga
    35581 tttcatcacg ctggaaagct acgggtcggg gacggaatcc tcgggccagg tgcgggtgac
    35641 caaggacatc accctggacg tgcgcggcgc gcacgtgctg ctggtggacg acatcttgga
    35701 caccggccgc acgctctctt tcgcccggca atggatcggg gagaagggcg cggccgccgt
    35761 ccggacctgc acgctgctgg acaagccttc gcggcgggtc gtgccctgcc gggccgacca
    35821 cgtggggttc gcggtggaaa accacttcgt cgtgggctac gggctcgact acgacggccg
    35881 ctaccgggaa ctgccgtaca tcgccagggt cacgttcctc gacgagcccg gctgatcccg
    35941 tctttcgccg ctcactcgac caggtcgatc aggtcccgca cgggatcctt cggctgggac
    36001 tgggccggcg cgcttccggg ctggggcggt tgggatgcat ccccgccgcc gttcccgccg
    36061 ccgtcggggc gaccccggcc gccgccatcc gaacgtccgc cgccgcgcgg acggcgccgg
    36121 cgccgggaat tcatgcggct gtcgccgcct ccgccgccgt ccggccgccc gccgccaccc
    36181 ccggtatcga aggcccggcg gaaagtgccg ggacggataa gggagccgcc ttgctcggca
    36241 accttcttct ccacctcggg gtgagccgag accacgaccg cccggcccgc gcccgcgcgg
    36301 cggaacagtt cgacgatccg gtccgggagc gccgccgcgt tttccacgaa gaaaacctgg
    36361 acggggccga aattctcgcc ctcggcgacc tcgcgcagcg ggcggccctc gaaaaccgcc
    36421 tgggcctgta tcttttcccg ctccacgaac cgcgaaagct gctgcagcac ctgcacctgg
    36481 tcgcgcggac ttaaccggtc cccgggacgc gccgccccgt gcagcggcgc cgcgtccagg
    36541 atgtagatcc ttcccgcccc gccgctcttc gcgccgaata acttctttaa accatccaac
    36601 aaactcatgc tcgctcctgt tcgcccgttt ccgccgggaa tacggcggaa aacacatggt
    36661 ggccggacac tatcacggcc ctcaaagccg tagcaagggg gatttgacgg cgcccgccgc
    36721 gtcaggcctg gaccagccac tcgcaggcat agggccgcag ggccaggcgc ccgccctcgg
    36781 gatgcgagcg cgccgggaat cccggcagcg cttcccaggc ggccccgtcg ggcagagacg
    36841 cttcgatcgt ccgcccgctg cagttgtgga gggccaaaac gcgctggcca ccctccgggg
    36901 ccgcgcgctc aagagcaaac agcgcgggat ccaggtccag gacggcctgg gcggcgtccg
    36961 gatgaaaagc cgggcaggcc gcccggcatt gcagcagggc gagcagatcg cgaagcaggc
    37021 gcgacgcggg cgaggcggga tcgtcgaggc gcgacgccag ttccgcctcg ttccatgcat
    37081 ggcggttgat cgagcgggcc cggcccgtgc gggaaacggc gtccggatcg ttgggggtgg
    37141 acgtcagggt gtgcaggtaa aacgccggga tgcccttcag ggccatcatc acggcctgcg
    37201 agcagaggaa ccgcgccgcg tgccgcccgg gatccggatc ggccatggcg ctgtaccacg
    37261 aaatgttcag ttcgtacgcg ctctcgctcc cgtcgctgtt ccgccggcgc gagacctgtc
    37321 cgccccggtc gcgggtttct ttttccatcc attccacctc gccgtcggga acgagcccgt
    37381 gcagcgggcg caacccgatg ccgtcgtgcg aggctgtgaa attcaggaag gtgcagcccg
    37441 gaggcgggtc ggcgagggcg gccgcccacc gggtcagggc ggtggcgtcg ccgcgccaga
    37501 acgcgtgcag gaggagcggc gggaggctga actggtacac catctgcgac tcgtcgcccc
    37561 ggccgaaata gctcacgttc tcctcgtgcg gaacgttggt ttccgtcagc agaatagttt
    37621 gcggggaaac gagttgcagg aaatccctca tcacccgcac gatctcgtgg gtctccggca
    37681 ggtggaggca ggaggtgccc ggttttttcc agaggtaggc gacggcgtcc aggcggatca
    37741 cgcgggcgcc catggcgatg tagaagagca ggatgtcgag tatctcgaac agaacatcgg
    37801 gattggaata atcgaggtcc acctggtcct cgctgaacgt ggtccagacg cgccggtcgc
    37861 ccgcgcgcgt ccgggcggcg tgcagcagcg gcgtgctgcg cggacggacc acggccgaca
    37921 ggtcggcggc cgggtcggcc tcgacgaaga aatgacggcc cggggcgacg cccgcgaggt
    37981 aatcgaggaa ccagccgctc cggcgggaca cgtggttcaa caccaggtcg aaggccaggt
    38041 tgaatcgctc cttcagggcg cgaacatccg gccagtcgcc gagggcggga tcgaccgtgc
    38101 ggtagtggac caccgagaac ccgtcgtcgg aggaggaggg gaagaagggc aggacgtgga
    38161 ccgtgctgat cgcgccggcc agccgccggt ccaggaatcg tttcagcgtg gccaggggtt
    38221 tttcgccggc ggtccggatc gaatccgcgt aggtgatgag ccaggcatcc ttctcgctcc
    38281 acgggggggc cgacggggcc tcctcgatgc cgtagcgtcc gaccagcatg gcgagccgcg
    38341 acaggcatcg gtccgcggcc ggcccgtaca gccgctcgaa ccgcgcgcgc agcctcgcca
    38401 tggtttcgct ggcccagtag cggctcatac gttcgcgaga ccccggatgt tgatgatcag
    38461 cgtgcgcagg cggcggcgca gcacggcgta gctgtagtgg cgaagagcca cctggtaatt
    38521 gtgctcgacc atgtcctgcc ggtacgcggg gtcctccaag atgcgccgga cctcggcgac
    38581 gatctcgcgc gtcaggtagc cttccatgat cggacaccgg aaccccttgg gctcaatgtc
    38641 ccgggcgaaa ttatcataac gatttaccag cacgggcttt ttaaaataca cggcctcgag
    38701 gaatgcgttg ccgaaacctt cgtagaagct ggggtaggtc acgaagtccg cgtggggata
    38761 gaggtcccag agcgtgtaca ttttctgccc ttgcccgttg acctgccggg tgtcggcgac
    38821 ccgcgtggcg aaaaagcgca cgtcgacacc ggccttcttc gcgtaggtcg tgagggtttc
    38881 gaggtagtcc gtgccctcgt cgccggcctc gtgcgagatg accagcttgc agcgcttgtc
    38941 cgccagcgcc tggaccaggc tgatggcatg cccgatgccc ttgcgcggca cgatgcgggt
    39001 cggctgcagg atcaggatat cgtccggcgc gagaccgagc tcgacgcgcg cgtcggcggc
    39061 gtagccgtcc gagggcggag gcggattctc gaagtccagg acgttgggca cgagcagcga
    39121 gggcacggcc ttccggcgcg ccagctcctc ctgggccgac tggttgatca ccacgtgctg
    39181 cagttcgcgg tcgcgcgagg ggaacgccat gtccaggtaa tcgttcacgg cgttgacgga
    39241 aaagcgggtg cgctcccagt agaaatcgtg atggtgcccg atggcgggga ggtgcgtctc
    39301 ggacatcagc tcgctcaagg ccaggcccag gggcacgtgc atggggatgg ccagcacgtt
    39361 ctcgaggacc atgatccgga catcgaaccg gcgcaggaaa tcgtagagcg tctccttgag
    39421 gtactcgctc atcgcccgga tgcggcgggt gaccagcggc gagcgggcgg tcttgcccca
    39481 gatccgctcg ttgatccaga ggttttccgg gtggccgaaa aacgcctcgg ggacgcaccc
    39541 gctggcctcc ggcggcgcct cgaggcggcc cgcgtaccag aagcagcggt ggccttcccc
    39601 ctccaggacg cgcgcccact tgtacgcctc caaggtcacc ccgtccgtgc cggcgaagcg
    39661 cgtggaaata aagccgatgt tctcgatctc cgccgtcacg ccgcccgcct tccgcgactc
    39721 cctggttcgg tgaccttcac accattattt tagtccggcg cagacgcccg gtcaatcgcg
    39781 cccgcgcaat ccggcccttt ttctctcgac gcccgcgcgc ccgcgcgcta tgaaaaggac
    39841 gcgcccatga gttcaatgga acaagtactg gacatcctgc cggccgcccg gaaggcggcg
    39901 gatgacctgc gcgacctgct cctggccaac gcggtcatga tcggcgaaat tccggccgcc
    39961 accgggaacg aacagcgccg cgtggacttt ctcctgcaac ggttcgcgga gagcggcttg
    40021 tcgtcctgct cttccgatga acggcgcaac ggggcggccc tcctgcccgg gtcggacggg
    40081 cggcgccata tcctgctggc caccggcgcc gacaccggcg tgcccgacga cgcggatcag
    40141 accgtcgagg tggagccgga ccgccttgtc ggcccgttcg tcgtcgataa cagcatcgcc
    40201 ctcgcggtcc tggccacgct gccgctcctg ctggagcgct tgcagctccg gctgcgatcc
    40261 cacgtcctgt tcctggccgc cgcccgcagc ctgggccgtg gcaatctgga cggattgaag
    40321 tacttcctcg ccaacgcccg ggcgccagtc cacttcgggc tctgcctcga aagcgtccag
    40381 ctcggccgcc tgaaccacgc ctgcctcggc atgctccgcg gggaaatcgt ctgccgcctc
    40441 ccggacgatt acaactgggc ccagcacggc gccaccggct ccatcatccc gatgaacgat
    40501 gtcatcacgc gcatcaaccg catccccgtg ccccgccgcc cgttcaccag catcgtgctc
    40561 ggctcgatcc acgggggcat ctcccaccag aatatcgcgc gccagaccac gctgggcttc
    40621 gaggtccgca gcgaatccgc ggacatcctc cacgagatcc ggcagaggat ggaggacctc
    40681 gtcgccgagg tcggcgccca gtcgggcctg cgcgtcgacc tcgacttctt cgcccggcgc
    40741 gagcccggcg gactcgacat cggccacccg ctggtccgct gcgggcgcgc cgtgctggag
    40801 cggctcggcg tgacgccgac gacctaccgg accacctcgg gcatgagcgc catggtcgac
    40861 cggggcatcc cggccctgac cgtgggcgtc accaccgggg aacggctggg ccggctggac
    40921 gagttcgacg aggcgtgcgc catcgggccc atggccgcgg gcctggcgca actggtcggc
    40981 atcctgctcg ccatggacgg aggccacgcc gatggacccg cgtgactggc tcgcccgcaa
    41041 cacgttccac cactcggcct tccaggatct ccggcgactg gtcgcggaga aggagcggca
    41101 gggcctgcgc ctctcgctgt gcatccctac gctcaacgag gagaaaacca tcggcaagga
    41161 gatcatgctc ctccgcgcgg agctggtcag ccggtatccg cttttcgacg aaatcgccgt
    41221 gatcgactcc ggctcctcgg accggacgcg cgaggtcgcg gcggcgttcg gcgcggacgt
    41281 ctacgagtcg gcggacatcc tgccggagac gggcacacgg cgcggcaagg gcgaaaacct
    41341 ctggaaggcc atctaccagc tcgcggggga tatcatcgtc tacctggacg ccgacataaa
    41401 gaacatccgc ccgcactttg cctacgggct cgtcggcccg ctcatcgagc ggcccgaggt
    41461 gcggtacgtc aaggccttct acgaccggcc gctggcgggc gccgggggaa cccggccgtc
    41521 gggcggcggg cgcgtgaccg agatcctgat tcgcccgctg ttctcgctgt tcttcccgga
    41581 cctcgccgcc ctcatccagc cgctgtccgg cgagtacgcc gtgcgccgcg agacgctcga
    41641 gcggctgtcg tttcccatcg ggtacggggt ggaaacggcc cacctgatgg acgtgtaccg
    41701 cgcgcacggg ctcgaggcct tcgcgcaaac cgacctcgac cggcgggtgc accgcaacca
    41761 gtcaacgggg gatcttggcc gcatggcctt cggcatcctt cagaccttcc tctcccggct
    41821 ccggcccggc gggggcgcgg aggccctccc ggaactgcac gccatgctcc gccagttcca
    41881 ggcgcgcgac aacgcctacg agctcgtcga gcaccatatc gtggaagagg agcggccgcc
    41941 catgattgac ctgccggctt accggcagcg gcggggcctg ccgccgtgac cgccgccccc
    42001 cagccggacg cgcgaatcga atgggtgcgg cgcctcgccg cgcgctcggc gccgatggcg
    42061 ccccggcccg tcgggatcga gccccgtacc cccgccttgc ggggcatccg ggccgtgctc
    42121 ttcgatgtct atggcaccct gctgatctcc ggcgccggcg atatcggcct ttcgctggac
    42181 gccgtccgcg ccgacgccat ggccggggcc ttcgaggccg cgggcctggg ccgcctcgat
    42241 gacgagcccg cccggcgcgc cgcggaacgg ttcgccgccc tcatccggcg acgccacgag
    42301 gagcggcgcg cggcgggcgt cgaccgcccg gaagtcgata tccgggaaat ctggcgcgcg
    42361 ctgctccgcg aaatcggcgc ggcgccgaag gaagaccaaa tcggactcct ggccatcgag
    42421 tacgagaccc gcgtcaaccc ggcctggccc gtgcccgggg ccttcgaact cgtggagact
    42481 ctcgcgaaac ggaacctcgt tctcggcctc gtctccaacg cccagttcta taccccgctg
    42541 ctcatcgagg cgctcaccgg ccgcgcgccg gaagcctggg gttttgcgcg ggacatgtgc
    42601 gtgtggtcct tcgaagaggg cgcggccaag ccgtcgcccg gcctgttccg cgcggcagcg
    42661 gagcgcctgc gccgaagcca cggcctggaa ccgcgcgccg cgctggtcgt cggcaacgac
    42721 gcggccaacg acgtcgctcc cgccgccctg ctgggatttc gaacggcgct cttcgccggc
    42781 gacgcccgct cctaccggcc gcagcccggc cacgccccgc cggatctgct gctcaccacg
    42841 ctgccgcaga tcgcgtcctg cctggccgat tgattccaga tccgggatcc cggcggccgg
    42901 acccggttga cgggccaagg gacgacgggt acgctccctg tcctgaaacg ccatgtccga
    42961 actgaaaaca taccagatcc gctgcccgcg ctgccggcag gagcagccgg cggggctcta
    43021 cgagtccatc aacgtcaaga ccgatcccga cctgcggcag aagctcatgg ccaacgcgat
    43081 caacgtggtc cgctgcgagg gctgcgagct gtcgttccgc gtggacaaac cgctgctcta
    43141 ccacgacccg tcgcggcgca tcatgatcta cctggtcccc atggccgacg acgccgtcga
    43201 gaacagcgag cgcctgtttg cccagctgct gatgaacctc gaaaacgtcc tgcccgccga
    43261 ggtccggccc ccgacgatcg ggctcgtttt caaccgcacc gagctcgttg aacgcatctt
    43321 cctgctggag gccgggctca atgagcggat catcgagtac ctgaaatacc tgctcttcac
    43381 gcgcaacgcc gaccggttga atcccgcccg gaaggaactg ctcttcaatg ccgaggactc
    43441 caccgcggaa tccctctgtt tcgtcgtgca ggacgtggcc acgcgcaagc tggaggccat
    43501 gctgaagtac gaccggggcg tgtacgccac gctcgcggag atgttcgacc gggatgagca
    43561 gaccccgcgc ctgctggagc tcttcccggg gccgcgcatc agcgcgcggg cgctgctgct
    43621 caaggagctg gggcgggagc cggcgtctcc ggaatcgtaa ccgccggggc ttccggcgcg
    43681 ggcgccgcgc tgtcggcggc gaccggggcc agcgggatcg atgacatttc cgggatcgtc
    43741 accgccgggg ccggcgcggg cgccggaacc gccgcgggac ccgcccggat ctgccggttg
    43801 atctggtcca gggcgttctc cgcctgcggg agccagccgc tctcgggatt cgcggcaatg
    43861 aaatcctcgt acgccgcccg cgcctcttcc agcttattgg cctgggccag cacgcgggcc
    43921 ttgttgagcc gcgcgagggg gaccaggtaa tcgtccgggt gcgccgcgac gaacgcatcg
    43981 aaggcctgtt ccgcctggtc catctgcccc atcgcctcca ggcactgggc gcgtcccagt
    44041 tccgcggcgg acttcatcgg atgcttggcg tacttctgct cgaactgcgc ataggtatac
    44101 tgcgccagct cgaactggcc gccgtcgaac tggcgggccg ccagcgtcag catggccatg
    44161 ggggccgccg gcgtggaggg atactggctg accacctgct gcaattgctc gaccgaaccg
    44221 gcgcgcgcga gcagttgcgc ggccttcacg acgttgttct ggcggtagga gcggtacgcg
    44281 ccgaatccca ggaggaaggc cgcggccaac cccaggccga tcagcgcggg ctgcccgtat
    44341 tcgcgccaga gggcgcgcag cctgtccgtg tccgtccgag gcgccggcgc cgcagacgga
    44401 cacggaaaag gtcccggcgg agccacaccg ggaatcggca gacgaaataa acggcggggc
    44461 gccggcaggg caacgacgag ccagggacgg atcgtctcag acttcccaag aacgtgagca
    44521 gctaccgcgc agtggcaggg tcaaggttgg ccgtcggggg ttagaagttc tccgggtgaa
    44581 gagggcccgg actgcggtta cggtccgccg tgtgaaagta acagacagta aacgacttac
    44641 ctagacacaa gatcattcaa ccaaaccgct ttgagtaagg agatgcgaat gcgcaagatg
    44701 atgaaggttc tggttccggt gttcatgctg gcgctggtgg ttgccggctg cagcaagggt
    44761 ccggtggagc cgcagaccaa catgccgctg acggcctcgt atgcgttgcc gatcgatgcg
    44821 gcgctggcgc tggcgacgct gcacatctat gttgtgggcg cgacgaacca tccggtcacg
    44881 gcgcaccggg tcaccgcggc ctgggatgag atgacggtca cctggaactc gttcggcgcg
    44941 gccttcgatg cggccgtggt tgacggtttc atgaccgatg ccggctggca gacgaccgac
    45001 gtgacctcgc tggtgcaggc ctggatgaac gggacctacg ccaactacgg gattctgctg
    45061 gacgaggccg acgatcagac cccgcggtcg atttacgcct cgagcgaaag cgctgacttc
    45121 ccgac
//
LOCUS       DAOOTK010000015        44329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_638_length_44329_cov_6.054163, whole genome shotgun sequence.
ACCESSION   DAOOTK010000015 DAOOTK010000000
VERSION     DAOOTK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 44329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44329
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_44329_cov_6.054163"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..3755
                     /locus_tag="PK634_03325"
     CDS             21..3755
                     /locus_tag="PK634_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOW96797.1"
                     /translation="MTRKRLGNGTFADYRYDALNRLSAILHYGPTTNLLIRFSYSYDP
                     RGNVSSCDTLEGIEYYAYDEIGQLTNATGSRDSWMRRYAYDAMGNRTSSFDGQSEWAY
                     GVNNLNQYTNIGASACSYDANGNLAQRQAAGVTNRYTFDAENRLVRVETASVTNEYGY
                     DPLGCRVLIRNGTGAVYQLWSGGQVVLEESAAHGTLGRWVWGAVPDEVVRMDRGAART
                     YYAQDARQSVVGLTDASGGLCEEYRYAPFGEPLGHGTNSNPWRYGGLAYDDVTGLQYH
                     RARYYSPDMGRFLSVDPMGMAGGINIYAYARNSPLQFVDRTGLGVGDMVEIYRAMGEA
                     ALTFSGAILDAVLAVAEAVVYVSTVSVQMQWAYSLSGADGGASFGAGNITHATPTTSS
                     RSAAIGGKYAVRAMTGYGYCPTFVQPPAGSAVIGGEAAFDPQDRGLAARIIVPPDGSL
                     LRSDIPVYGYAGGRNFARFRVEFGRGRDPAEWHLLRSSDHAEESPPDLRDVSWMQGDL
                     DLRGNLATWNTGLKNWEHLPWHPADDPTDLNGAYTVRLVVEGKDGQTVEDRVTCEVGR
                     AIAQCLPGRVVSPDGVATMRFPEQGLLDKFRVFTILPFSELDRPVPEAPAGMQLLTEP
                     YRVREPGEKFIKDIVLEFAAPPGGTAAPDRTGLCRYDATTQAWIWLPTDRDESGKTYR
                     TILQELPAPDAVYALAADATPGRSTVAQAISPPEPLIPAGPGILLDNTFDRDLGSIRP
                     RDRLVGAALARVERAAGDGCLELKKERFGGNFSFTVLDRPFDVREYPQFSFDYRIDQD
                     IKIDIYLRVHGRWHRLRFTDDPVDTCGQDLNISTLGAIPGIMADGQWRSASVDLARFL
                     RRKTRHTQVEEIIFADWDVGGYMALEFGRNRRDATFWLDRIRLAAGGREHDGAVLCLD
                     DYDRDDGRNRLGLPAASYGSDGQACQSSRAQANSQTGSCLALDVDVSRRGAYGGYWSG
                     LGDRDLRGYDWLCFDIRCDEGLRPAVSLKDADGRETAISMGRYVSSAGGDGWSRARIP
                     LQGFSESVDIERVAVVAFGFSDRQEVRKGRVLLDNVRFEPQDSSPAPELAVADFEGAA
                     WERNLMGGRCYTFENGAAGIRCSSIAARAGDDQGRSLMVAYGGSIGLDLGPDGFSYCG
                     WGARLGGLDLSGYQVLEMRLRGQEGGEKPNVYLGDGVVKRGLDVEKYSGISTGWAVVC
                     LPLADFAARGIDLTHVENMEMTFEWDRMSGSLWVDDVGFR"
     gene            3776..5350
                     /locus_tag="PK634_03330"
     CDS             3776..5350
                     /locus_tag="PK634_03330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96798.1"
                     /translation="MMRRDSLYLGAIGWLALLLTILLAGMLEWRQGSPLIGALWIVGF
                     FFVALNLCYLVVVCLVSIRLRARRLPEAEPVRAVRTAILYVVKNEPGDFAGRMRQTMA
                     GNDGPLNELWLISNSDDEALRRSEERVVRALREEFGPHRAHHYRPGSNPLGRKHVAIA
                     QWARAHREYDYFIVCDADSILYPGAVARLVAKAEHPANRRFMVFQSHLQIAGSRTRFA
                     RFLEPGQNIVQKAFCEVNFALFGQSPYYGHGALIRRAPFARLRVPAAVLSHDLWDTAA
                     IDRAGWGIAFCSDVHTHEMFPADYLEFRRRSRRWIVGTLEAWPLLFRRRLTLGTRFHL
                     ALANYIYIVQPVFLLWVLLGLLAHNSLAGPLLKTQAVFLGGSAALDFEMGGISVATVG
                     LMWAYKQRFARGLREALLVLRDAATGTAVLLNNLFYDSIFILLAPWTARSWRPMRKEA
                     VPERTFAECFRAMLPSTAFGAALLAGSLALDSRWTLYSSPILFSFLFGSALVYWTSRP
                     ARAACPDNGAGIRGAV"
     gene            5347..7254
                     /locus_tag="PK634_03335"
     CDS             5347..7254
                     /locus_tag="PK634_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96799.1"
                     /translation="MSILSRCRAGAAFAVAFLAAGTDGAWAGPTEFLFEPNPVYAATN
                     WEFTTDLRIRAPDRNPGAFQIVLVYEPDVLRILDVTPSPSTPFYSYFQADAASFSKGY
                     TRIAALQTQDLSDSMDEVVLRVQWAAIGSGARTSDVSVYVEGLVDVRWRETSGAGTPL
                     AVDVRGVDTNANTIPDAWEEGRYGAPTNAAPGSDEDGDGYTLEQEYWADTDPRDPDSL
                     PLVGVATAGPNAAVFCEGSRLRRYHFDVQDSLVSGTWSNWFSVQPSGDGTIWLLDHDR
                     ETNRFYRLRVQLPISGAAGGGETNEHWTSDAVTDFTALQPLLYRLHPGGAYTLTALDL
                     LSSERFEFRFNLANGSSRANTDWAGGMLWFDGTPFDASVNGIVLGLSASGTTEVKLEA
                     TDTGGRKAVVILGALSRNERNYRVPAAELLAAGGSGFDPGHIRAIAVVVDGAVAGWSR
                     ASGLVNVRTKGVPYDQKSNILLSGTAYDTNALSSFGSYYPAWVPDGGNTSEEGSNGII
                     VATGMPEARGFRYEYDLTADPGAFAYVAIHRGGVLLFRLPAIYTFAAKGTAGARMRVT
                     YRDGDEHMGQVVVLLEEGFRNYSIDFSTMAPADFDRLRVKELTFVQDWTIGTPFLEDA
                     VQIETPGLDYP"
     gene            7423..10239
                     /locus_tag="PK634_03340"
     CDS             7423..10239
                     /locus_tag="PK634_03340"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96800.1"
                     /translation="MLIPCAALADTHFVSASGSAPLAPYTNWAHAASSIQDAVDEAAP
                     GDTVLVADGVYTQGGRAVFGTMTNRVAIDKPIVVRSVNGAQSAAIAGRGASLGSNSVR
                     CVYVGSNATLEGFTLAGGGTHITGDRHHELSGGGAWCETGGLVRLCVISSNVAGYGGG
                     VYGGAVRDSMLCGNEANTGGGACSSRVENCTVVNNRAFHAGGGISAGTIANSIVYYNV
                     ASSDPNWQPGTLSYCCTLPLPPGDGNISDEPGILSFDNPHLVSTSPCIDAGDNGLVAG
                     DADCDGEGRIEGSRVDMGCDEVWSGGITGALSVAISAVSTQAITGYELRFRSVIAGRA
                     EGYEWILAGSSLPGAPVVARNWSVTGTFPVVLRAWNTGAACCATTTVAILAGFTNYAS
                     PRGGHLPPYTNWASAATSIQAAVDAVQVAGGVTMLATGVYEQGSYLTALMRCRFGIDK
                     PMTVRALDPTPWATVIRGQGPLGTSAIRCAYLTAGAVLEGVTLTEGHTRAEGALGDGE
                     LAGGGVFCDGGGTVSNCLIAGNAAHDGGGSFGGTLAGCTIGSNTAAQFGGGAFGSMLR
                     DCLVHDNSAGWGGGCSEGTAERCMIFRNTATNYGGGVFNVRISDSVVFSNHASAGGGA
                     CAGILRNCTVAGNRAALLAGGTLLGEAENSIVVDNMDAWGASNYSGTAFFYSCTTPMP
                     TNGTENQSSAPGFADGPAHTYRLQPWSPCIDRGMNQSWREVAIDVEGAPRIVNGRIDL
                     GAYEFHFGAALRVFLQGPYSSNLDAMTTALASNLPFASPYAADPAAVTEIPSNVVDWV
                     LVQARPGLTNAPTFTRSAFLRSDGAVVSSAGETSLVVEVSPGTTNYLAISHRNHLSAL
                     SAAPIVFTNQAVQYSFTTGAGQYYGGTNAAAQLEPGVWGLMAGDADGDGKITAVDRSI
                     CTSQVGRTGYLGGDFDLDGIVYDK"
     gene            10236..13277
                     /locus_tag="PK634_03345"
     CDS             10236..13277
                     /locus_tag="PK634_03345"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96801.1"
                     /translation="MNNGEKLLFSAVIICMYLITAASADTLYVSTNGLHQTPFTNWMI
                     AARTIQDAVDAATDGDMILVSNGIYNLGGREAGGEHVLTNRVIIDKPITLQAVGGPEH
                     TIIMGSKDPSSDGYGDAAVRGVWMVDGSIIVGFTIEGGSTRTSIGADDNAYGSGAFCQ
                     STNAVISNCVLKGNSAAGIGGGSYGGTLIHCAITSNRASYGGGVYGSAVLASAFDGNW
                     ASDGLYGSNGGGADGSVLQNCMLSGNRADYGGAASESTLCNCFLSGNTAFLGGGSYEC
                     TLNNCTVVGNSAEYDGGSHGDRLYNCIVYYNDAPSGPNHGGSTFEHSCSAPLPSGPGN
                     ISREPGLASASHLALASPCKGAGSSLYASGYDIDREPWLSPPSMGCDELHIGSITGAL
                     NVSIWASYTNVAVGIPVQFRADAVGRTTASVWEWGDGVVSSNQVYAEHVFTSTADYEV
                     VFRVYNESKPSGVAAGVSVHVASRMVHYVNLNSETPSAPFESWETAATNIQDAIDAVS
                     QIGALVLVTNGIYATGGRMIYGALTNRVVVIKRITLQSVNGPAVTVIQGAHDPFTTNG
                     NSAVRCAYLGAHAIMSGFTLTNGSTRTEGDYPSDYRGGGVWCEAGSIVSNCIVAGNTA
                     YFDGGCDGGTINDCLLTGNMALGGLVGWFGGGGSGGGTLNRCILSENYSGDQGGGALY
                     GTLNNCLLVHNSARTGGGAYLCSLNNCTVVGNTASSEGGGVYDGELNNCIVYSNAAPT
                     GSNHNDSVFIRINNSCTIPALAACSNITSDPKLTDQFELTANSPCIDAGVNQEGMAET
                     RDLAGVSRIINGKVDMGAYEFHYNATLQAFLQGPYCTTLHTMKVILPSNMPFSAPYAG
                     DPRAVLQIPSNVTDWVLLEMRSDPSNSAIFSESAFLRNDGRIINAAGETNIIIEVSPG
                     TTNYLVISHRNHLSAMSAQPIVFTNQAVQYSFTTGSGQYYGGTNAAAQLEPGVWGMMA
                     GDADGDGKITAADRSICESQRAQAGYLAGDFTLDGVVTDED"
     gene            13360..16629
                     /locus_tag="PK634_03350"
     CDS             13360..16629
                     /locus_tag="PK634_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOW96802.1"
                     /translation="MLTLYADTHYVDLYNPTPLPPFTNWPGAANSIQAAIDEASDGDT
                     VLVSNGMYVAGGRTLPGVSPVNRVIVTQSITVASVNGPSAAAIVGQGPLGTSAVRCLY
                     LNHPGALVSGFTLTNGHTATMFGTAGGGAYIESGVLSNCIVASSHANDYGGGVHGGML
                     YDCWLKGNTAGKWGGGAIYSVLSNCTIEANTAAWGGGARSCTLYNCRVIGNLATNSGG
                     GAIGGTIYDSELRGNTAISYGGGVYGATIFRSQIANNSALAGGGVGEDCEVHASVLSS
                     NIAQYGGGAGAARLYSCRLEHNVATGTGGGASGGSLFSCAVYNNQAQWGGGTQSSSNW
                     NCSIVANTASGSGGGVRFSWSRNCIIYDNDAPVQPNHEGGDTAYSCTTPAISASGNMS
                     AAPHLVTPFRLASSSPCLGAGSEEYMVGADLDGEPWRIPPAMGCDELYPANLTGSVIL
                     SVIGEFAQAATDFAVVFRADTLSAVASNLWDFGDGVRLTNYLHASHAWSSGGLYTVSV
                     TAYNLNHPTGVTAAATIMVVDHPVHYVNVHSPSPAYPFTNWTTAATTIQSAVNAAIPG
                     SLVLVTDGIYNAGGAITPQGILSNRVVITNGVALRSVNGPDSTFIVGAGPLGSNAMRG
                     VFLSNQGRLDGFTITNGHTRFTDGVIAADKSGGGIFASGGIVTNCIITGNSARTAGGA
                     HFGEYYNCGFFGNRALNYVIWGVIFDGSGYGGGVNQAMLHRCWIEDNLAEDGGGGAGS
                     SQLFNCIVRNNRAFTGYEDKGISASGGGAYRSSLYGCTVISNSAALIGGVDNCRVVNS
                     IVYYNTASQAQWGHNYTTNRYSASTFSGSCTTPHPADAGNISSPPEFADDTGGDYHLA
                     WHSSCVDAGVFFEGIEGLSDYDGRPRVFHRPDIGAYEYVMETDIRVKLAGASGPFSGD
                     MRTDLARLGYVPMHSLYAATQIPRGEMPSNATDWILVELLDPQSGVSMAAEQAILRCD
                     GTVLSDQGGAGLHLEASPGSYFLTIKHRNHLSVMSAQPLDYTNATVFYDFTTGPEKAR
                     DGTNSLVEVTPGAWGMIAGDADGDGKITAVDRSVCTSQVGRTGYLAGDFNLDGVVTTN
                     E"
     gene            16622..19714
                     /locus_tag="PK634_03355"
     CDS             16622..19714
                     /locus_tag="PK634_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOW96803.1"
                     /translation="MNNCEKLLFSAVIICRLLITAASADTHYVSTNGLHQTPFTNWVF
                     AATNIQDAIDAASDGDEVLVSNGVYATGGRVVYGAMTNRVMITNAVTVQSINGPTVTI
                     LQGAGPVGSNAVRCAYVGAHASLVGFTLTNGATWSSGDEFISQSGGGLWCEPGGEISN
                     CVITGNSAASHGGGVYQGSLHNCTVDGNYAGWWGGGAYKSALHDSTVSSNSSFANGGG
                     AYFCSLSNCTVTGNSSEGGGGTLGGALYNCILSRNYADVGGGSFGGELYNCSLTDNSA
                     NWGGGSSQGELNNCTLFANCARLGGGGTESGRLRNCIIWFNSAASGANWNGGTLNYCC
                     TMPIPPGEGNLGCDPLLASAWRLGGASPCIGRGSAAYASGVDIDGESWATPPSMGCDE
                     RSGSVTGDISVAIMAKYTNFAVGFPVEFTAQIEGHPAESWWILGMDGVVSNRPIINHS
                     WASTGTYVVILKAINESYPAGNSASATVNVVLAPVHYVNQSNTFPDSPFTSWHTAATT
                     IQEAVDAATVPGATILVTNGLYDTGGRVINGDMTNRVVIDKPVVVRSVNGPLVTFIRG
                     RGPIGNGAVRCAYVGRNAELAGFSLTGGCTMAMTDERTERCGGGVWCESQAVVSNCVI
                     TGNSASSAGGGVAQGSLFNCTVSGNSASGAGGTYVSFLHSCILSNNSATGEAGGAYQS
                     MLDGCILVDNAAREGGGVIQSILFNCTLLGNSATDSGGGAFQSTLFNCTVVGNRAASA
                     GGTLQGSANNCIIFNNTGGNYVQGTIQYSCTTPMPSGTGNITDTPHFRASNDFHLAAN
                     SPCVDRGQNQQWMVGACDLDGSPRSVNGTVDMGAYEFHFDGNLKAVLQGPYDTNAHLM
                     ATLLSSNVPPTSPYVADARTVGNIPSNVTDWIMLQVRPGITNNPVYARSLFLRNDGRI
                     LSDQGDTNLLIEVSPLTTNYLVVSHRNHLSAMSAQPIIFTNQQISFDFTSSSDQYYAG
                     TNGAVELEPGAWGMIAGDADGDGKITAVDKAMCEGQEGQTGYLAGDFNLDGFVSTNEP
                     "
     gene            19831..23601
                     /locus_tag="PK634_03360"
     CDS             19831..23601
                     /locus_tag="PK634_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96804.1"
                     /translation="MKKVLAALALLCWGAGLQGRAISNDLYFVTNNMGRVTSVPNGEV
                     ILYPQLVVSPPRKTLVAQSTQVMTAQGSTNPITWFMIERPSGGTITVTSPTSVTYQAG
                     ATSDCVDIVEAWDGGNMFGRAYLNVISPAEVAAAGKAIIVAGRKGADDPLWPATDYLA
                     NNAFNTLLYRGYGKEHVQYLNPVPGRDVDGNGTADDIDLESTLGNARLTFTNWAGNTH
                     RLFVYLVDHGEAASTNGYFRLNAGETLPGTTLKGWLDDIQNAYTTEVTVVVDCCYAGT
                     LINQLSYTGVARRILIASSGTNEPAYFIGGGLVSFSDAFFGGIMLGYDVLRAYEQARS
                     AMLTYQGPAYTDNGSGTEGTNVWLGATFVAGKDIPQIGLVCGRQLLMDGTTANLWARD
                     VVSSYPIERVWCMVVPPSHNPTNAADPVQDIPELALVYTNDPGRYHQDYNGFSEEGTY
                     KIIYYAKDIWGSVSLPRQSYVDQASYDERVLLVAGDATNAADGASIRNMANDAWHSLL
                     ERGFATNRLRYLSHEAASQVATSPSLSAISYAITNWAGTTNWGGPADRLTVYIVGQGA
                     NSQLRLNSGEWLAASQLDQSLDLFQASNRTVNVIMDFAGSGAWIPQLAPPVERERINL
                     ASTRAGAPCLREGAGALSFSRFFWRAVFQGSSIGTAFDQARRAVWMASGSWGQTAQLD
                     DNGNGLANEKSQDRLIAQLRYIGPAFRTGNDAPVIGAVTPDTELTGTNRFMLWAQDVV
                     DADGVSNVWCVITEPGYNGRTGVVEVVLAWNAGTQRYETDYSGFSATGVYACTFYAGD
                     RGGEISSPRQAVIRTREGNGVDADEYETDDLPEQAKPLLVGETQTRTLHVSNDVDWAV
                     FFVGDQTNYFHTIRTDHLTNTVDTVLDIYQEGDDGALTLIQHVDQWDPDQGESWGWYP
                     SNGVYFARVSSTNFTEPGGYELALTIDIGGPFDHLLVLAMNGLTAGAVPTGVKAHMAG
                     SDLPFNGAYSVDFVGPDNGTHTVTLTGVPAGWYYVQGYPAAQNVTVPNTPGKAYYAQF
                     VLLPCFGITGTVRDAWTGEWLDGAALSFQATSGRIAGQTSASNWYSQVGGGLPGGVRV
                     ATVNWAVTLAKAGYSNAVKSIAGAGIAAGNSTNWGTVWMVPVDSSGAGLPDAWRDLYF
                     TGAVDPGGDADGDGQSNEEEYWANTHPTNGASLFMYYQAPAAAATGFTLTWPAASGRD
                     YAVERAATLVPGDWVRVHGPQTASAGQTTMSWTDTNAVQGCCYRIVLYPPAP"
     gene            23615..24286
                     /locus_tag="PK634_03365"
     CDS             23615..24286
                     /locus_tag="PK634_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96805.1"
                     /translation="MGIMKRHGWAGRAPVATWRKAIHLAALLVGVTGRIGRGQDADGV
                     ALDVTQLKTLAQQHEQAGEKQAAADIYEIIMAREPSSRQILAARLAILCAEMGATNKA
                     LAWAHEVASANPDPEAYLAGIYEKLGDLTKAQAILKDLVQQEAGSRVMLARFWQLAAV
                     YEKMGQLESSEAMLKRAYEAASSPAEKDCAVARWNQFKQRRETSLDVASPVDPAVPSG
                     SSPMK"
     gene            24461..28519
                     /locus_tag="PK634_03370"
     CDS             24461..28519
                     /locus_tag="PK634_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOW96806.1"
                     /translation="MLAHHLQIIFERKAFCIRLARQKRRRTMKSANLLARTWASITTT
                     ISMVKGGISVFLISHPQWAQLSPSLIVEASPLRRPDLFCRVGKASRPRSIVMLRTIMI
                     AGLGLPHIALAQKAASAHGEAFASATVMEQIAAMGREKNSWTPVQRKISSKLLLETRQ
                     RLGRPITAGLPEIRSAVEVDAAGLTLVDIKAEVSDGLLRRIQHLGGQVINSHVRYSSI
                     RALVPIAQLEILAGETAVKSIKPSDKLTTHKVDTSQGVVTHCADSARTSYGVDGTGVR
                     VGILSDSVESLASLQASGDLPAGVTVLSGQAGSGRSEGTAMLEIVHDIAPGAGLYFAT
                     ANGGQAQFAQNILALQAVGCKVIVDDCSYYEEPVFQDGIIAQAVEIVTAAGCIYFSAA
                     GNEGNLNDGTSGVWEGDFVAAAGRPTPITSGTAHDFGAGINYNTITVDSPSFFNLKWA
                     DALGASANDYDLYLLNSTRTTIYDYSVDLQDGNDDPYESIDSFKFNDTGNTLVIIQKT
                     GGKTRFLHLSANRGRFSRSTAGQIGGHSAAASALSVAAVDVATAGGGVFVGGAANPVE
                     RFSSDGPRRIFYMADGTPITPGDFSSTGGALRQKPDIAAADGVSCSTPGFNLFKGTSA
                     AAPHAAGIAALMIDSGLTTPAQIRQAMTSSAWDIETDGVDRDSGWGLVNALAALGYSI
                     AENVSAPTTPAGLSAGLTNQPLVFSAGGAKSSVGHAVQYRFGWGDGTLSPWTAGTATG
                     KWSAAGSYSVVSQARCISGHATSAWSAARLVAITNTPAGVLGAAAAWMEVPATMISGR
                     TYRVKVAMQNTGTIPWTLAGGFKLGSEMAVDTYRWGLTRVQVTNTVASGAVQVFEFDV
                     TPREVGGRPCAWRMVKEGYRWFGATNSSIVVVSAAASPLMRKINCGGPAVAGGWLADT
                     GCLAGQAYVVTAPIANAGAVAQAVYQSCRYGPGATPFGYSFPDVPNGNYKVKLHFSEP
                     LWTAAGLRLFHIDAEGQRKLSGLDVVKRAGGRNRALVIELVIAVRDGNGLQLQFAAVK
                     DCAMVSGIEIVQESVSLPRINCGGAGIGDFAVDRGGVGGQVYQSAAVIAGAGVTPQAL
                     YQTCRYGPAGAAFSYAFADVPNGAYKVRLHFSEPVWWKAGQRLFDVVIEGVRKEAGLD
                     VWSQAGGINRSLVKEYAVAVSDGNGLQIVFTNATRDCAMVSGIEVIQASAAADGGGLL
                     VRTAAPTAFSANGVDRAAPGPDPMSFSIPAPLDVVTSGNAFEDATGWAAVDQSHETAW
                     IGNPGGNGWWVALAYEPEITASQLELTLGAHSLTNVQCLCSEDADHWVDFHSAATNGP
                     VTLRYLWLVFPADGSDRVPVVLEIAPRVERGD"
     gene            28734..29627
                     /locus_tag="PK634_03375"
     CDS             28734..29627
                     /locus_tag="PK634_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96807.1"
                     /translation="MNLTNWPAMSFGMRPSRKMAWGLLMLVSATATGIRAAEYVVPTN
                     GAAAYGNTGTSYPCLSSACRRYQQVYAASEFASAQSPQLITRLGFRYRTATSTFYLSG
                     TWMEVRLSTTSRNPDGLSATFADNPGADETLVYAGTNWQFAGSSALGKFWMDAYFDRP
                     FHYDRTKGNLLVEIRMPGYYNFSGYTLDATNAASDSVSRLYASATNAAAGTLDSVGAI
                     TEFTFQDVVLSNISAEAVAGGIVLRHASISNAEYRVQVSTNLAGAFANASAIYTASPP
                     FNVVTQFPGSGLKFYRVVREK"
     gene            29782..30351
                     /locus_tag="PK634_03380"
     CDS             29782..30351
                     /locus_tag="PK634_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOW96808.1"
                     /translation="MKKMAAMLVGMMCLGGVAHGESRLGPFASWLTSNDENAFGVGLK
                     YEWLFNGKFGMDARAGYLTDSDMYVIPLELGVVGILPLERLSLYAGVGGGYYIPEDIG
                     MSTPWGPVDGPDATFGFYGVAGIRMPTGKNMEFFAEAKYTRAESDEESTSGPIYRNGQ
                     LVGYTVHQTTKMGMDLDGIGANAGILWKF"
     gene            30584..31009
                     /locus_tag="PK634_03385"
     CDS             30584..31009
                     /locus_tag="PK634_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96809.1"
                     /translation="MSTARSGRGTRGWIGPILAIVLIVAAGGAWWYFEKPAKPPDFGT
                     LAGRWVRPDGGYVVEIKAVGKDGALEAAYFNPKPIHVAVARAFTEAGALNVFIELRDE
                     NYPGSTYTLAYDPAADQLQGLYYQAVMGQRFEVYFERIK"
     gene            31012..32352
                     /locus_tag="PK634_03390"
     CDS             31012..32352
                     /locus_tag="PK634_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005299708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOW96810.1"
                     /translation="MAEVPRRDVWHPAGPGQPVEVFTSLDRYDAPLMMADRGRRRFHV
                     MIKPVGCRCNLNCAYCFYLHKADLPGGPGAGRMSDAVLERFIQQYIEGVTADEVVFSW
                     QGGEPTLLGVEFFRRVVELEKKHARPGQTIQNDLQTNGTLITEEWCAFLKANRFLVGL
                     SIDGPKKLHDRYRVAKDGAPTFDQVFRAARLLAKHGIPFNTLTCVHRENARFPLDVYR
                     FLRRELGSTYLQFIPIVEYKGFERTAPQKWNWERMPRDGEPEARPGHPDSVVTDWSVD
                     PDDWGYFLCRVFDEWRSRDLGKVLVNHFETLVAQHLGLPSQLCVYGEFCGKGVAVEQD
                     GSVYACDHYVYPEYRIGHVNEKPLADTVLSRAQVQFGYAKNEALPNQCRQCEFLKDCW
                     GECPKNRILRTADDEPGLNYLCRGFKRYFAHAVPEAERIADGLRRQPRESRRRM"
     gene            32412..32984
                     /locus_tag="PK634_03395"
     CDS             32412..32984
                     /locus_tag="PK634_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOW96811.1"
                     /translation="MKPNSIRKSGGAAITILLCLLCLGIGFAAGYLARRWQASGSSAM
                     PARCLRVVDTDTIVIHWLYGTNKVRMAGIDAPESKENKKLREQAEMLGIKPAALLQVS
                     QVVIRQMDIMLTGKDVRIVFPRGKVEYDSFGRVLAYIEVGGKDICEMLMRNGLVYPRP
                     EPHPRKERYAQVNAQAKAQRKGIYGMSGAK"
     gene            complement(32997..34670)
                     /gene="gpmI"
                     /locus_tag="PK634_03400"
     CDS             complement(32997..34670)
                     /gene="gpmI"
                     /locus_tag="PK634_03400"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOW96812.1"
                     /translation="MDTLKPLAGCRGPQGPVVLMIMDGVGIGRYEEGDFVRAASTPHL
                     DWLRAHVPFTQLKAHGTAVGMPSDEDMGNSEIGHNAIGCGRVFDQGASLVNKAIESRS
                     LFEGAAWRELVQNGLQKSSALHFLGLFSDGNVHSHISHLEAMLREARAAGVKKAFVHI
                     LLDGRDVPPQSALVYVDRLEAFLKELNAGGATDFAIATGGGRMKITMDRYEADWEMVK
                     RGWDIHVRGAGPAFPSARAAIEAGRAANPSVIDQDLPPFVIVRDGRPVGPIRDGDGVI
                     FYNFRGDRAIEISRAFEEDAFDKFDRGPRPAVLFAGMMQYDGDTGIPHRFLVPPPGID
                     RTMGEYLSATGVRQMAISETQKFGHVTYFFNGNRSGKFDDKLETYVEVPSDRVPFEHR
                     PWMKAAEITDKVIEAIERKRNKFRFIRLNYANGDMVGHTGIPQAVQVAVETVDLCLGR
                     VMKAVEKAGGILVVTADHGNADDMYEHEKKTGAVVMDPRTGQPKIRTAHSLNPVPCGI
                     YDPSGASCVRLSAAAQGGGLGISSLAATCLTLLGHEPPADYTPSIVDAG"
     gene            34811..35701
                     /locus_tag="PK634_03405"
     CDS             34811..35701
                     /locus_tag="PK634_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOW96813.1"
                     /translation="MSAEASGAGEPRIEEHLEAVRSRIMQVMTATAVGPLVAGYRRLL
                     VQGKMLRSRLAWRVGEALQADPAMLVDAGAAIEMIHAASLLHDDVIDGGYLRRGLPAF
                     WVEQGIPGAILLGDLMLFQALRLLSAPGQRALLGEAIRLTGEVCEAESEQELMMREVP
                     STWEAGVDVARRKTGALFALAAGAGAGPETPSLDALRESGFLVGTAYQLADDFLDAHG
                     DAETAGKTLGSDAGRNKATAFRAAAEAGIDVPRYLEALLAQSVDGLARWPTVRAAWAR
                     YLETDLRPALDHNLRCQAAL"
     gene            35787..37079
                     /locus_tag="PK634_03410"
     CDS             35787..37079
                     /locus_tag="PK634_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96814.1"
                     /translation="MRWIRGNRWAVAVVLLAALVVAGTLARVAYRATRVWSVGADFQE
                     RLRYLVETGQTIGEKPASETFSVDYLQTIFGDNPELLEHLKSVVQQGMAQDSTLTAGE
                     VTALLVTYHLQDDGSVADVIVHAVGGFPAARKRPGFHRHGYFFQQIDNDLWTLGNIAI
                     GFLGRDIIVFGADEAATERQRQILDSIFSGEIMMLVAHLQEPLYFSLVLPDPRQAVPP
                     QLRNHVQAVVVKGYLGHNDGRCDILVLTPSPRSSRYALNLFADMKTASEAALRSKWGG
                     VKKMNEWGMPEYDWWAFEMVKTLEQATLEQEFSIIRMGTTFDRVMINAILKSIERMSR
                     DLAAMQGIEDERLDPRLVDARLRNSNPEVPTTYPLHYWSAQHQWGPDWPIPATTNLPP
                     LTSSSETPAPAESPVPAEPAAGTEVPASPAAPATPATP"
     gene            37301..38659
                     /locus_tag="PK634_03415"
     CDS             37301..38659
                     /locus_tag="PK634_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96815.1"
                     /translation="MLLSLATWTPLAWAAPAEYTVKQVSDDHFFSRDPVISDTGFAAW
                     SAYHSEDGEDSASDIHVYDNGQQRSLTGGNVPVGTTHYRPCVYSNTAAWIGSFPYPKT
                     GEGNWVLKEVPAEVRDAPYEELVAFPEYMTTNAVPTEPVTNAPAAETPGEPPPGAPAD
                     EAAAEEVGGPDSARRHPSGVMESVAWFGDEIKRISYDWRNDFGVSLWGRTLAWQKSKG
                     FPFGWEIMVWNDGTMYQLTTNFFYDMAPQVQGTQIAWYGWDGNDYEIYLHDFAAGTTM
                     AITSNRYDDVSPVLWDGQVAWEAYPGADAEVYLWKEGRFQRLSDNPWDDTNPRIWNGK
                     VVWQGFDGDDFEIYLFDGVKSVKLTSNRHDDVNPDIRDGLVCWMAYYDNWDSEIFAWD
                     LDKGEYIAMINEEGQVGSTPGRLSYNEVEDREPRTAGRRVIWQVQQEGKSLIMLAEPK
                     AP"
     gene            complement(38665..40200)
                     /locus_tag="PK634_03420"
     CDS             complement(38665..40200)
                     /locus_tag="PK634_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOW96816.1"
                     /translation="MLARVRSGAVLGVDAFAVEIEVHAGRGDPATIVVGLPDAAVKES
                     KDRVHTALINSGFMPPRGRTTINLAPADIKKEGPSFDLPIALGMLVAEEELAPGRLDG
                     LCVLGELALSGEIRRVRGVLPIAMEARAAGCRGLMIPEDNAEEAAVVEGLPVYPVRTL
                     RQAADFLAGTLALEPYRLDARAVFDQASHGDEDFADVKGQESAKRAFEVAVAGGHNIL
                     TLGPPGTGKTMLARRVPSILPPMTLEEALETTKIHSIAGILAAHQALVALRPFRAPHH
                     TVSDAGLLGGGSHPMPGEVSLAHNGVLFLDELPEFHRNVLEVLRQPIEDGHVTISRAA
                     ASVTFPSRFMLVAAMNPCPCGYFGDTRKECRCSPIQIERYRHRISGPLLDRIDIHVEV
                     PAVAYTELSRAEPGEPSSAIRGRILCARELQRKRFEGARRVYCNAMMRSRDIHKHCPI
                     EDEAQHLLKMALTELHFSARTYDRILKVARTIADLDGSETLRTPHVSEAIQYRSLDRR
                     YWV"
     gene            complement(40214..41431)
                     /locus_tag="PK634_03425"
     CDS             complement(40214..41431)
                     /locus_tag="PK634_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96817.1"
                     /translation="MRFPGNQILAVWLAVWAGLSAAAEPGRPPDFPTDWTNLNELAQF
                     HAEETQYLLEHKDLDAASLEVQQALSLAPDHPTLLWWAADLYTKRGQYAMSESYWARL
                     SELYPSNAWVFARWGNVFFELDRFDRAQVALARAIELDPAQLSSRYLLASIHLMNGRR
                     DLAEPLLEGLPLDEVRQMATWIQTDADLLAGKFGYRPLEALCQLALGRRSAGPAESGS
                     EPTVEEWKARMGSVAVALAGAMEALTNRQWAAVRDGLEAAREAGLESPDATRDHALAL
                     YRLGEKTRAIEELEQLIAQRPEWPGAQRIYGLVLLEEREYDKAVEALVEARKMADSDA
                     ETAFALICAYSAARRFTDARDTARALPPEQARQVAAWAAGGHPYDSLLETNRDLRTWL
                     RNLGAEPPPATPD"
     gene            41575..42510
                     /locus_tag="PK634_03430"
     CDS             41575..42510
                     /locus_tag="PK634_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOW96818.1"
                     /translation="MKKHAIAAVLMMIPALAGWADPVKVAILDFEDRTGQASDALLGG
                     AIKSGALADKGLDLLGRRLLDAETFVLVDRRDFINQIEQLQPTDSGAPTPTKPSFLHA
                     AQALNMDVVLRGSLLSFSPGKQTVRQGGYETEFVTLSLRISVEAVDPVDGTVIAMADG
                     AAAGQFRQTAEHFTSLSEDDALGLLDKAVDQVVPVLKAKLDQRQAAQAARPRVKISVK
                     SSADPALVEIDGILVGSTPLENFEVYQGDHVLTVGKPGHRDITKRILLEQDSAIEVPM
                     MRTEMSAEEWKDILEKVRMNVVIGEPGIVITPLAE"
     gene            complement(42621..>44329)
                     /locus_tag="PK634_03435"
     CDS             complement(42621..>44329)
                     /locus_tag="PK634_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW96819.1"
                     /translation="DADVDAPEAWDITTGSNSIVIAIIDDGVEWEHPDIIDNLFANEP
                     EGIPGVDGDGNGYTNDLHGWDFVNDNNDSGPKDADDQHGMCCAGVAASAGNNGLGVAG
                     IAYGCNILPIKVIHGTNWVTPSAMASAIRYSAGLDVDGTNRWRGADVISMSLGFTALP
                     VTDAAFDDAVTKGRGGKGTPVLASSGNNASGHSYFTRNPSGTNGTPCYIEFWYRKNDS
                     VTNGEDAVWVSSVKLPDAGGTWERFDSPDLPAGWSVTGDVPFSIVDDPSHAYGSGRYA
                     ARSGAIGDSQGSCLRTPAFYTDRDKALEFQAWVSSEKTKDWLTVEWHYLNTGNTNIEY
                     ITSGIPGDRMWLPGDHEIDYYIHYPASHSNVIAVGASTDFDYRSDYSQYGTGLEFVVP
                     SSGGHLSPLEDTHQIVTIDRTSTNGYNTAEGSDGDYTATFGGTSAACPLAAGIAALIL
                     STDTNLTWQEVRDRMRASCERIGPVPYDENGWNEYYGYGRLNAWAAVAGIPWDLVLTN
                     PVVTDTRTYRAFNTITAFDQFRVQSPGDVTLNAGNKITLGPGFQAVSGAVFRTRLGEV
                     TP"
BASE COUNT         7938 a        13474 c        14744 g         8173 t
ORIGIN      
        1 accgggatgc cggcgggcgc gtgaccagaa agcgcctggg gaacgggacg tttgcggact
       61 accgctatga tgccctcaac cggttgtccg cgatcctgca ctacggtccg accacgaacc
      121 ttctgatccg gttctcctat agctacgatc cgcgcggcaa cgtgagttcc tgcgatacgc
      181 tcgagggcat cgagtactac gcctatgacg agatcggcca gttgacgaac gcgacggggt
      241 ctcgcgacag ctggatgcgc cgctatgcct acgacgcgat gggcaatcgc acgtcgtctt
      301 tcgacggtca gagcgagtgg gcctacgggg tcaataatct caaccagtac accaatatcg
      361 gcgcctccgc ctgcagctac gatgccaacg ggaacctggc gcaacgccag gcggccggcg
      421 tgacgaaccg ctacacgttc gatgccgaaa accgcctcgt gcgcgtggaa accgcctcgg
      481 tgacgaacga atacggttac gatcccctgg gctgccgtgt cctcatccgg aacgggacgg
      541 gcgcggttta tcagctttgg agcggcgggc aggtggtcct cgaggaaagc gcggcgcacg
      601 gcacgctggg ccggtgggtg tggggcgccg tcccggacga ggttgtgcgc atggaccggg
      661 gcgcggcgcg cacctattat gcccaggacg cccggcagag cgtcgtgggc ttgaccgatg
      721 cctcgggggg gctgtgcgag gaatatcgat acgcgccctt cggagaaccg ttggggcacg
      781 gcacgaacag caatccgtgg cgatacggcg gattggccta cgatgacgtg acgggcctgc
      841 aataccaccg ggcgcggtac tactcgccgg acatgggccg gttcctgtcc gtcgatccga
      901 tggggatggc gggtggcatc aatatctacg cctatgcgcg caattcaccc ctccagtttg
      961 tggaccgcac cggtcttggc gtcggcgaca tggtcgagat ctaccgggcc atgggggagg
     1021 cggcgctgac gttcagcggc gcgatactgg acgccgtgct ggcggtggcc gaggccgtcg
     1081 tctacgtgag cacggtcagc gtgcagatgc aatgggcgta cagcctatcg ggcgcggacg
     1141 gaggcgcctc cttcggcgcc ggcaacatca cgcacgcaac gccgacgacc agcagccggt
     1201 cggccgccat cggcgggaag tacgccgtcc gggccatgac gggctatggc tactgtccca
     1261 cgttcgtgca gccgccggcc ggatcagcgg tcataggcgg cgaagcggca ttcgatccgc
     1321 aggaccgcgg gctggcggcc aggatcatcg tgccgcccga cggcagcctg ttgcgatcgg
     1381 atatcccggt ttacggctac gccggcgggc ggaatttcgc gcgcttccgc gtggaattcg
     1441 ggcgcggccg cgatccggcg gagtggcatc tgctccgttc ttcagaccac gcggaagaga
     1501 gccctcccga tttgcgggac gtgtcctgga tgcagggcga cctggatctc cgcggcaacc
     1561 tggccacgtg gaatacgggg ctcaagaact gggagcacct gccgtggcat ccggcggacg
     1621 acccgaccga tctcaacggc gcctacaccg tgcgcctggt cgtcgagggg aaggacgggc
     1681 agacggttga agatcgcgtg acatgcgagg tgggccgggc catcgcgcaa tgcctcccgg
     1741 gccgtgtggt cagccccgat ggcgtcgcga cgatgcgctt cccggagcag ggcctgctgg
     1801 acaagttccg cgtcttcacg atcctgccgt tttccgagct cgaccggccc gttccggagg
     1861 cgccggcggg catgcagttg ctgacggagc cgtaccgggt gagggagccg ggggagaaat
     1921 tcatcaagga catcgtgctc gaattcgccg ccccccccgg cgggacggcg gccccggaca
     1981 ggacgggcct ctgccggtat gatgcgacaa cacaggcgtg gatctggctc ccgacggatc
     2041 gcgacgaatc cggaaagaca taccggacga tccttcagga actgcccgcg ccggatgcgg
     2101 tctacgcgct cgccgcggat gcgacgccag gccgatccac cgtagcgcag gccatctcgc
     2161 ccccggagcc gctcatcccg gcggggcccg gtatcctgct cgacaacacg ttcgatcggg
     2221 acttgggctc catccggccg cgggatcggc tggtcggcgc ggcattagcc cgggtggagc
     2281 gcgccgccgg cgacggatgc ctggagttga agaaagagcg gttcggcggc aatttctcgt
     2341 ttacggtgct ggatcgcccg ttcgacgtcc gcgagtaccc gcagttctct ttcgattacc
     2401 gcatcgacca ggatatcaag atcgacatct atttgcgggt tcacgggcgc tggcaccgcc
     2461 tgcggttcac cgatgacccg gttgatacct gtggccagga tctcaatatt tcgacgctcg
     2521 gggccatccc cggcatcatg gcggacggtc aatggcgctc ggcgagcgtg gatctggctc
     2581 gcttcctgcg aaggaaaacc cggcacacgc aggtggagga aatcatcttc gccgattggg
     2641 acgtgggcgg atacatggcc ctggagttcg gccggaacag gcgtgacgcg acgttctggc
     2701 ttgaccgcat acgcctggcg gccggcggcc gggaacacga cggcgccgtt ctgtgtttgg
     2761 acgactatga tcgcgacgac ggccgcaatc ggctcgggct tccggcggcg tcctatggtt
     2821 cggacgggca ggcctgccag tcttcccgcg cgcaggcgaa ctcgcagacc ggctcctgcc
     2881 tggcgctgga cgtggatgtg agccgccgcg gggcctacgg aggctactgg tccggcctgg
     2941 gggaccgcga cctgcggggc tacgactggc tctgttttga catccggtgc gacgaaggac
     3001 ttcggccggc tgtttcgctg aaagacgcgg acgggcggga gaccgcgatt tccatgggcc
     3061 ggtatgtctc gagcgcgggc ggggacggat ggtccagggc gcgcatcccg ctgcaaggat
     3121 tctccgaatc ggtggatatc gaacgcgtgg ccgtcgtggc ttttggcttt tccgatcggc
     3181 aggaggtccg aaagggacgg gtgctgctgg acaacgtgcg gttcgagccg caggactctt
     3241 cgcccgctcc tgaactggcg gtggccgatt tcgagggcgc ggcttgggag cggaacctga
     3301 tgggggggcg gtgctacacg tttgaaaacg gggcggccgg cattcgctgc agttccatcg
     3361 ccgcccgggc gggagacgac cagggccgca gccttatggt ggcgtatggc ggcagcatcg
     3421 ggctcgatct ggggccggat gggttctcgt actgcggttg gggcgcccgg ctgggcgggc
     3481 tggacctgtc cggataccag gtcctcgaga tgcggctgcg cggccaggag ggcggagaga
     3541 agcccaacgt gtacctgggc gacggcgtgg tgaagcgcgg cctggatgtc gaaaaataca
     3601 gcggcatcag cacgggctgg gccgtggttt gcctgcccct ggccgatttc gccgcccgcg
     3661 gcattgattt gacccatgtg gaaaacatgg aaatgacgtt cgagtgggat cggatgtcgg
     3721 gaagcctgtg ggtggacgac gtgggcttcc gataaaacgc gtgcagacga ggggcatgat
     3781 gaggcgcgat tcgttgtatt tgggagccat cggatggctg gccttgttgc tgaccatcct
     3841 gctggccgga atgctcgaat ggcggcaggg gagcccgctg atcggcgccc tgtggatcgt
     3901 cggcttcttt ttcgtcgcgc tgaatctgtg ctacctcgtg gtggtttgcc tcgtctcgat
     3961 ccggctgcgc gcccgccgcc tgccggaggc ggagccggtc cgcgccgtcc gcaccgccat
     4021 cctgtatgtc gtcaagaacg aacccggcga tttcgccggg cgtatgcggc aaacgatggc
     4081 cggcaacgac ggcccgctca acgagctgtg gctgatatcg aattccgacg acgaggcgct
     4141 gcggcggagc gaggagcgcg tcgttcgggc gctgcgcgag gagttcgggc cgcatcgcgc
     4201 gcaccattac cgaccgggct ccaaccccct cggccggaag catgtggcca tcgcgcaatg
     4261 ggcgcgcgcc caccgcgaat acgactattt cattgtctgc gacgccgact ccattctcta
     4321 tcccggcgcc gtcgcccggc tggtggccaa ggcggagcat ccggccaatc gccggttcat
     4381 ggtgttccaa tcccacctgc agatcgcggg cagccggacg cggtttgccc gttttcttga
     4441 gccgggtcag aacatcgtgc agaaggcctt ctgcgaggtg aacttcgccc tcttcggcca
     4501 gtcgccctac tatggccacg gcgcgctcat ccggcgcgcc ccgttcgccc ggctgcgcgt
     4561 gccggccgcc gtgctcagcc acgatctgtg ggacacggcg gccatcgacc gcgcggggtg
     4621 gggcatcgcc ttctgctccg acgtgcatac gcacgaaatg ttcccggccg actatctcga
     4681 gttccgccgg cggagccggc gatggatcgt gggcaccctg gaggcctggc ccctgctgtt
     4741 ccgccgccgg cttacgctcg gcacgcggtt tcatctcgcc ctggccaact acatctacat
     4801 cgtgcagccc gtgttcctgc tctgggtgct cctgggcctg ctggcccaca acagcttggc
     4861 gggcccgctg ctgaaaacac aggccgtgtt cctgggcggg tcggcggcgc tggatttcga
     4921 aatggggggt atctcggtgg ccaccgtggg cctcatgtgg gcctacaaac agcgcttcgc
     4981 gcgcggcctc cgcgaggcgc tgctggtgtt gcgggatgcc gcgaccggca cggccgtgct
     5041 cttgaacaac ctgttctacg actccatctt catcctgctg gcgccctgga ccgcgcgaag
     5101 ctggcggccg atgcgcaagg aggccgtgcc ggaacggacg ttcgcggaat gtttccgcgc
     5161 catgctgccg agcacggctt tcggcgcggc cctgttggcc ggcagcctgg ccctggattc
     5221 ccggtggacg ctctattcca gcccgatcct cttcagtttc ctgttcggca gcgcgctcgt
     5281 gtactggacc tccaggcccg cgcgggcggc ctgcccggac aacggcgccg ggataagggg
     5341 ggccgtatga gcatcctttc tcgctgccgc gctggcgcgg ccttcgcggt cgcgtttctc
     5401 gccgcgggca cggacggcgc ctgggccggc cccacggagt tcctgttcga acccaatccc
     5461 gtctacgcgg ccaccaattg ggaattcacc acggacctgc gcatccgggc gccggaccgg
     5521 aacccgggcg cgttccagat cgttctcgtc tacgagcccg atgtgctgcg catcctcgac
     5581 gttacgccca gcccgagcac gcccttctat tcctatttcc aggccgacgc ggcctcgttc
     5641 tccaagggct atacccggat cgcggccctc cagacgcagg atctctcgga ctccatggac
     5701 gaagtggtgc tgcgcgtgca atgggccgcc atcgggtccg gggcgcggac ttcggatgtg
     5761 tccgtctacg tggagggcct cgtggacgtc cgttggcgcg agaccagcgg cgccggcacg
     5821 ccgctggccg tggacgtgcg cggggtggac acgaatgcca acacgatccc ggacgcctgg
     5881 gaagaagggc gctacggcgc gcccaccaac gcggcccccg gttcggacga ggacggcgac
     5941 ggctatacgc tggagcagga atactgggcc gacacggacc cgcgcgaccc ggactcgctg
     6001 cccctggtgg gagtggccac ggccggcccc aacgcggccg tcttctgcga ggggtcgcgt
     6061 ctgcgccgct atcacttcga cgtgcaggac tccctggttt ccggaacctg gtcgaactgg
     6121 ttctccgtgc aaccgtctgg cgacggcacc atctggcttc tcgatcatga ccgggagacc
     6181 aaccgcttct accggctgcg cgttcagctt ccgatctccg gggcggcggg cggcggcgag
     6241 accaacgagc attggacctc cgacgcggtg acggacttca cggccctgca gcccctgctg
     6301 taccggctgc atcccggcgg cgcgtacacg ctgacggctc tggacctgct gtcttccgag
     6361 cggtttgaat tccggttcaa cctggccaat ggaagttcca gggctaatac cgattgggcc
     6421 ggcggcatgc tgtggttcga cgggaccccg ttcgatgcgt ccgtcaatgg cattgtgctc
     6481 ggcttgtccg cgtccggcac aaccgaagtc aaactggagg ccacggatac gggcggccgg
     6541 aaggcggtgg tcatactcgg cgcattaagc cgtaatgaac ggaattacag ggtgccggcg
     6601 gccgaactgc tcgcggccgg cggcagcggc ttcgatccgg ggcacatccg agccatcgcg
     6661 gttgtggtag acggcgcggt ggccggctgg tcccgggcga gcgggttggt gaatgtccgg
     6721 acgaagggag ttccatacga ccagaagtcg aatattctgc tgtccgggac ggcctacgac
     6781 accaacgcgc tgtcgtcatt cggctcgtac tacccggcat gggtgccaga cggagggaat
     6841 acgagcgagg aagggtcgaa cggaattatc gtcgccacgg gcatgccgga agcgcgcgga
     6901 ttccgctatg aatatgatct gactgcggat cccggcgcgt ttgcatacgt tgcaatccat
     6961 cgcggggggg tgctcctctt tcgcctccca gccatctaca cgtttgcagc caagggcaca
     7021 gcaggagcgc gaatgcgagt gacgtatagg gatggagatg aacacatggg gcaggttgtc
     7081 gtgcttctgg aagagggatt tcgaaactac agcattgact tcagcaccat ggctcccgcg
     7141 gattttgatc gtttgcgtgt gaaagagctt acattcgttc aggattggac cataggcacg
     7201 ccgttcctgg aagatgccgt gcagattgag acccccggct tggactatcc ctaggctccc
     7261 gacatcctga ccaacaggag ttggccgcgt gcgcgcgaag agaggccccc gggctggtga
     7321 atagaaggtt ttcttcgtga atccatcgac aagaaaacga tgaaaagtgt tcgacagctt
     7381 ggagccgccg cgtcggcgcg ggcgacgtgt ttagctggtt gcttgctaat cccctgtgcg
     7441 gcgctggccg acacgcactt tgtcagcgcc agcgggtccg cgccgctggc tccctacacc
     7501 aattgggcgc acgcggcgtc gagcattcag gacgccgtcg acgaggcggc gccgggcgac
     7561 accgtgctgg tcgccgacgg cgtctatacc cagggcggcc gcgcggtctt tgggaccatg
     7621 accaaccggg tagccatcga caagcccatc gtggtccgca gcgtcaacgg cgcccagtcc
     7681 gccgccattg cgggccgggg ggcctcgctc ggcagcaaca gcgtccgttg cgtctacgtg
     7741 ggcagtaatg caacgctgga gggcttcacc ctggccggcg gggggaccca tataacggga
     7801 gatcggcatc acgaattgag cggcggcggc gcctggtgcg aaaccggcgg cctcgtgcgc
     7861 ctctgcgtca tctccagcaa cgtggccggc tatggcggcg gcgtgtatgg cggcgccgtg
     7921 cgggacagca tgctctgcgg caacgaagcc aataccgggg gcggcgcctg cagcagccgg
     7981 gtcgaaaact gcacggtggt caacaaccgc gcattccatg ccggcggcgg catcagcgcg
     8041 gggaccatcg ccaacagcat cgtctattac aacgtcgcca gttcggaccc gaactggcag
     8101 ccggggacgc tctcgtactg ctgcaccctg ccgctgccgc cgggcgacgg caacatctcc
     8161 gacgagcccg gcatcttgag cttcgacaat ccccatctcg tgtcgacttc gccctgcatc
     8221 gatgccggag acaatggcct ggtcgcgggc gacgcggatt gcgacggaga gggccgcatc
     8281 gaaggttcac gcgtggacat gggatgcgat gaggtctggt ccggcggcat caccggggcc
     8341 ctgtccgtcg cgatctccgc ggtatctacg caggcgataa cgggctatga actacgcttc
     8401 cgctccgtca tcgcggggcg cgccgaggga tacgaatgga tcctggccgg cagttcgttg
     8461 cccggcgcgc cggtggtcgc gcggaattgg agtgtcaccg gaaccttccc ggtggtcctt
     8521 cgcgcctgga acacgggcgc ggcatgctgc gcgacgacga cggtcgccat tttggccggc
     8581 ttcaccaact acgcctctcc gaggggcggg caccttcccc cctacacgaa ctgggcgtcg
     8641 gccgccacgt ccatccaggc ggcggtggac gccgtgcagg tggcgggcgg tgtcaccatg
     8701 ctggcgacgg gcgtctacga gcaggggagc tacctgacgg cgctcatgcg gtgccgcttc
     8761 gggatcgaca agccgatgac ggttcgggcc ctggatccga cgccatgggc gacggtcatc
     8821 agggggcagg ggcccctggg aaccagcgca atacggtgcg cctacctcac ggccggcgcc
     8881 gtgctggaag gtgtcacgct gacggaaggc cacacccgcg cggagggcgc gctgggggat
     8941 ggcgaactgg cgggcggcgg ggtcttctgc gacggcggcg gaacggtgtc gaattgcctg
     9001 atcgcgggaa atgccgcgca cgatggcggg gggagcttcg ggggcacgct ggccggatgc
     9061 acaatcggca gcaacacggc cgcgcaattc gggggcggcg cattcggcag catgctgcgc
     9121 gattgcctcg tccatgacaa ctcggccggt tggggaggcg gctgcagcga ggggaccgcc
     9181 gagcggtgca tgatcttccg caacaccgcg accaactacg ggggcggcgt gttcaatgtg
     9241 cgtatttcgg actcggtcgt tttcagcaac cacgcatcgg cggggggcgg ggcgtgcgcg
     9301 ggcatccttc gcaactgcac ggttgccggg aacagggcgg ccctcctggc cggcgggacg
     9361 ctgctgggcg aggcggaaaa ctcgattgtc gtcgacaaca tggacgcgtg gggcgcctcc
     9421 aattacagcg gcacggcttt tttctactcg tgcacgacac cgatgccgac caacggaacg
     9481 gaaaaccagt cctccgcgcc cggcttcgcg gacgggccgg cgcacaccta tcggctgcag
     9541 ccctggtcgc cctgcatcga ccggggcatg aaccagtcct ggcgggaagt cgcgatcgac
     9601 gtggaaggcg ccccccgcat tgtgaacgga cggattgacc tgggggccta tgaattccat
     9661 ttcggcgccg ccctgcgggt gttcctccag gggccgtaca gctcgaacct ggacgccatg
     9721 accacagccc tggcgagcaa ccttccgttt gcctcgccct atgccgccga ccccgccgcc
     9781 gttacggaaa ttccatccaa tgtagtggat tgggtgctgg tccaggcgcg cccggggctc
     9841 accaacgcgc ccacatttac ccgcagcgcc ttcttgcgca gcgatggcgc cgtggtgtcc
     9901 tccgccggcg agaccagcct ggttgtcgag gtgtcgccgg ggacgaccaa ctacctggcg
     9961 atcagccatc gcaaccatct ctcggccctg tcggccgcgc ccattgtgtt cacgaaccag
    10021 gcggtgcaat actcgttcac caccggagcg ggccagtact acggcgggac caacgccgcc
    10081 gcgcaactgg aacccggagt ctggggcctg atggccgggg atgcggatgg ggatggaaaa
    10141 atcacggcgg ttgaccgatc gatttgcacg tcgcaggtcg ggaggaccgg ttatctcggc
    10201 ggcgattttg atttggatgg tattgtttac gacaaatgaa taatggcgaa aagctgctgt
    10261 tttcggcggt gataatctgc atgtatctga tcactgcggc atcagccgac acgctttacg
    10321 tctcgaccaa cggccttcac cagacgccgt tcacgaactg gatgatcgct gcacgcacta
    10381 tccaggatgc agtcgatgcg gcaacagatg gcgacatgat tttggtgagc aacgggattt
    10441 acaacttagg tggacgggag gcgggcggtg agcatgtgct gaccaatcgc gtgataattg
    10501 acaagcccat cactctgcag gctgtaggag gtcccgaaca cacaatcatt atgggatcaa
    10561 aagatccaag tagtgatgga tatggcgatg ctgctgtgcg cggtgtctgg atggtagacg
    10621 gctcgatcat tgtgggcttt acaatcgaag gaggctcgac gaggacatcg attggcgctg
    10681 atgataatgc gtacggtagt ggcgcttttt gccaaagcac aaacgcggtg atatccaatt
    10741 gcgtgttaaa agggaattcg gccgccggta ttggcggcgg gagttatggc ggcactttaa
    10801 tccattgcgc aatcacgtcg aacagagcga gctatggcgg gggagtgtat gggagcgcgg
    10861 tgcttgccag tgcattcgat ggaaattggg cctcggacgg cctctatgga agtaatgggg
    10921 gtggcgccga tggaagcgtc ttacaaaact gcatgctgtc ggggaatcgc gccgactatg
    10981 gcggtgctgc cagtgaaagc acattatgca attgctttct tagcggaaat acggcgtttc
    11041 taggtggcgg ctcttatgaa tgcactctga acaattgcac ggtggtcggg aattccgctg
    11101 aatatgatgg gggatcgcat ggggatcgtc tgtacaactg cattgtatat tacaatgatg
    11161 ctccctcggg gcccaatcat ggcggttcga cgtttgagca cagttgttct gcgccgctgc
    11221 cgagcggccc cgggaacatt agtcgtgaac ctggcttggc aagcgcgtct catttggcat
    11281 tggcatcgcc ttgcaagggc gccggcagca gcttgtacgc atcaggatat gacatcgata
    11341 gagagccttg gctaagcccc ccgtcgatgg ggtgcgatga attgcatatt ggctccatca
    11401 ccggggcgtt gaatgtcagc atatgggctt cctataccaa tgttgcggtc gggatacctg
    11461 tgcagttccg agcagatgct gtcggtcgga caacggctag tgtgtgggaa tggggcgatg
    11521 gggtggtgtc tagcaatcaa gtgtatgccg agcatgtttt cacctctact gccgactacg
    11581 aagtggtatt tcgtgtttat aatgaaagca agccatcagg ggttgctgcc ggcgtttcag
    11641 ttcatgttgc gagtcgtatg gttcactatg tgaacctcaa cagcgagacc ccttcggcgc
    11701 cgtttgaatc ttgggagact gctgcaacca atattcagga cgccattgat gctgtttctc
    11761 agattggcgc attggtcctt gtaacaaatg gcatctacgc aacgggtggc cggatgatct
    11821 atggggcttt gaccaatcgc gtggtcgtaa tcaaacgcat cacgctgcaa agcgttaatg
    11881 gtccggccgt gactgtcata caaggggcgc atgatccatt cacgactaat ggtaattcgg
    11941 cggtgcggtg tgcctatttg ggggcgcatg ctatcatgtc gggcttcacg ctaacgaacg
    12001 gctcaacacg caccgaaggc gactatccca gcgactatcg aggcgggggt gtatggtgtg
    12061 aagcgggcag cattgtatcc aattgcattg tggccgggaa cacggcgtat tttgacggag
    12121 gctgcgatgg cggaaccata aacgattgcc tcctaacggg aaacatggcg cttggaggcc
    12181 tcgtaggatg gtttggtggt ggcggctcag gtggtggcac gctgaatagg tgtatactga
    12241 gcgagaacta ttcaggggac caggggggcg gcgctctcta tggaacacta aataattgtt
    12301 tacttgttca caattctgcc cgcacaggag ggggcgcgta cttgtgcagt ttaaacaact
    12361 gtaccgtggt tggcaatacc gcttcaagtg aaggtggcgg ggtatatgat ggggaactga
    12421 acaattgcat tgtctactcc aatgctgcac cgacaggctc aaatcacaac gattccgtgt
    12481 tcatcagaat caataacagt tgcacaattc ctgcgttggc cgcatgcagt aacataacta
    12541 gcgaccccaa gcttacagac caatttgaac ttacggcgaa ttctccctgc atcgatgcgg
    12601 gcgtgaatca ggaaggcatg gccgagacaa gggatttagc tggggtatcg cggatcatca
    12661 atggcaaggt cgatatggga gcttatgagt tccattataa tgccactttg caggcatttc
    12721 tgcagggccc ttactgcaca accttgcaca ccatgaaggt aatcctgccg agcaatatgc
    12781 ccttttccgc tccgtatgcc ggagatccgc gcgccgtttt gcagattccg tccaatgtga
    12841 ccgattgggt tttgctggag atgcgttccg acccgagcaa ttcggcgatc ttctccgaaa
    12901 gcgcctttct gagaaacgat ggacggatta tcaatgcggc aggcgaaacg aatatcatca
    12961 tcgaggtgtc gccagggaca acgaactacc tggtcatcag ccaccgaaat cacctgtccg
    13021 ccatgtcggc ccagcccatc gttttcacga accaggcggt acaatactcg ttcaccaccg
    13081 gatcgggcca gtactacggc gggaccaacg ctgccgcgca actagagccc ggcgtgtggg
    13141 ggatgatggc cggggatgcg gatggggatg gaaagattac ggccgcggac agatcgatat
    13201 gtgagtcgca acgggcgcag gctggctact tggctggtga tttcaccttg gatggggtcg
    13261 tgacggatga ggattagccc acagtcgagt gttcatcgtt ccagttctgg gccgagaatt
    13321 ctgtggtttg caatgttgct gatgacggct cgggggtcaa tgcttactct ttatgccgac
    13381 acccattacg ttgacctcta caacccgacc cctcttccac cgttcaccaa ctggcccggc
    13441 gcggcgaata gtatccaggc cgccatcgac gaggcttccg atggcgacac tgtactagtc
    13501 agcaatggca tgtatgtcgc aggggggcgg acgctccctg gagtcagccc ggtcaatcga
    13561 gtcatcgtaa cgcaaagcat aaccgtcgcc agtgtcaatg ggccgtccgc tgccgcgatc
    13621 gtgggacaag gaccgctggg gacttcggct gtgcgttgct tgtacctgaa ccatcccggc
    13681 gcgctggttt cgggattcac cctcaccaat gggcataccg ccactatgtt tggaaccgct
    13741 ggtggcggcg cctatattga gagcggggtt ctctcaaact gcattgtcgc ttccagccat
    13801 gctaatgatt atggaggagg ggtccacggt ggcatgctct acgactgctg gctcaaggga
    13861 aacacagccg ggaaatgggg cggcggcgca atctacagcg tcctgtccaa ctgcactatc
    13921 gaggcgaata cagcagcgtg gggaggcggc gccaggagtt gcactcttta caactgccgc
    13981 gttattggaa acctggccac gaactccggt ggcggagcaa taggtggaac catctatgac
    14041 agtgagcttc gagggaacac cgcaatttca tatggtggag gggtttacgg cgcaacaatc
    14101 tttcgcagcc agattgccaa taacagcgcg ctggctggag gaggtgtggg ggaagattgt
    14161 gaggtgcatg ccagtgttct gtcctccaac attgcccaat atgggggcgg cgcaggagca
    14221 gcgcgcctgt attcctgccg actggagcac aatgttgcta caggcactgg tggtggcgca
    14281 agcggcggat ccctcttcag ttgcgcggtc tataacaacc aggcgcagtg gggcggaggg
    14341 actcagagtt cgtcgaactg gaactgcagc attgtggcca atacagcgag cggctccggg
    14401 ggcggggttc ggttttcatg gtcgcgcaat tgcatcattt acgacaacga tgcgcccgtc
    14461 cagccgaatc acgaaggtgg tgataccgcc tactcatgca ccacacccgc gatttcggcg
    14521 tcggggaata tgagcgcggc gcctcacctc gttactccct tccgcctggc ctcctcctcc
    14581 ccttgcctgg gtgccggcag cgaggagtac atggtgggcg cggatctgga tggggagccg
    14641 tggcgcatcc ctccggccat gggttgtgac gagttgtatc ctgcgaatct aacgggctct
    14701 gtcatcctgt ccgttatcgg agaattcgcg caggcggcaa cggattttgc ggttgtcttt
    14761 cgtgcggaca ccctctccgc cgtggcctca aatctctggg atttcggtga cggcgtgcgg
    14821 ctaacaaact atctgcatgc cagccatgcc tggtcaagcg gagggctata caccgtatcc
    14881 gtcacggcct acaatctgaa ccacccaacc ggagtcacgg ccgccgcgac gattatggtg
    14941 gtggatcacc ctgtccatta cgttaatgtg cattcgcctt cacccgcata tccctttacc
    15001 aactggacca ctgccgcgac aacgattcag tcggcagtga acgctgcgat ccctggcagt
    15061 ctcgtgctgg taaccgacgg catttataac gctggcggcg cgattacgcc gcagggcata
    15121 ttgtcgaacc gggtggtgat caccaatggt gtagcgttgc gaagcgtgaa cgggccggat
    15181 tcgactttca ttgtgggcgc agggccgttg gggagcaatg ccatgcgggg tgtttttctg
    15241 tccaatcagg gccgcctcga cggatttaca atcaccaatg gtcacacgcg ttttacggat
    15301 ggtgtgatcg ccgccgacaa gtcgggcggc ggcatctttg cttccggggg gattgttact
    15361 aattgtatca tcacgggtaa ctccgcacgg accgcgggcg gcgcccactt cggcgagtac
    15421 tacaattgtg gattcttcgg gaaccgtgcc ctgaattacg ttatctgggg tgtcatcttc
    15481 gacggcagcg gatatggcgg tggcgtcaac caggccatgc ttcaccgctg ttggatagaa
    15541 gataaccttg cggaagacgg gggcggaggc gcgggcagca gccagctctt caactgcatc
    15601 gtcaggaaca atcgagcctt caccggatac gaagacaagg gcatttcagc ctctggcggc
    15661 ggcgcgtaca gaagtagcct ttatgggtgt acggtgatca gcaattcggc cgccttgatt
    15721 ggcggcgtgg acaattgtcg ggttgtgaac tccattgtct attacaacac agcttcgcag
    15781 gcccagtggg gacacaatta cacgaccaac agatactctg ccagcacctt ctcgggctct
    15841 tgcaccacgc cacatcccgc cgatgcgggc aacatatcct ccccgccgga gttcgctgat
    15901 gacaccggtg gtgattatca tctcgcgtgg cattcgtcct gcgttgatgc gggcgtattt
    15961 tttgagggga ttgaggggtt gtccgattac gacggccggc cacgtgtgtt tcatcggccg
    16021 gatattggag cttatgagta cgtcatggaa accgacatcc gcgtgaaatt ggcgggagca
    16081 tcagggccat tttccggtga catgcgaacc gacctggctc gacttggtta cgtgccgatg
    16141 cattcactct atgccgcaac ccagatacct cgcggtgaaa tgccttcgaa tgcaacggac
    16201 tggattctgg ttgaactctt ggatccccag tcaggcgtgt cgatggccgc ggaacaggcg
    16261 atattgcgct gcgacggcac agtccttagc gaccaagggg gtgcaggtct tcatctggaa
    16321 gcgtctcccg gctcctactt cctcacgatc aagcatcgga accatctatc cgtcatgtcc
    16381 gcgcagccgc tggattacac caacgccact gttttctatg atttcaccac aggccccgaa
    16441 aaggcgcgag acggaacgaa cagcctcgtg gaagtcacgc ccggcgcctg gggcatgatc
    16501 gccggcgacg cggacgggga cgggaagatc acggccgttg accgatccgt ttgtacatcg
    16561 caagtcggac gaacaggcta tctcgccggt gatttcaatt tggatggtgt ggtgacgacg
    16621 aatgaataat tgcgaaaagc tgctgttttc agcggtgata atttgccggt tattgatcac
    16681 tgccgcatcg gcggatacgc actatgtctc caccaacggg ctgcaccaga cgccgttcac
    16741 gaactgggtt tttgccgcga cgaatatcca ggatgcgatt gatgcggctt ctgatggtga
    16801 cgaagtactg gtgagcaatg gggtgtatgc aaccgggggg cgggtggtat atggagccat
    16861 gaccaaccgt gtgatgatta ccaatgcggt gacggttcaa agcatcaacg gccccaccgt
    16921 aaccatcctt cagggcgcgg gccccgttgg gagcaatgct gtgcggtgcg cctatgtggg
    16981 tgcccatgcg agtctagtgg gatttacgtt gacgaacggc gcgacatggt caagcggtga
    17041 cgaatttatt tcgcaaagcg gtggtggctt atggtgcgaa ccgggcggtg aaatatcgaa
    17101 ttgcgtgatc accggaaact ctgcggcctc acacggtggt ggggtatacc aaggctcgct
    17161 ccacaactgc acagtcgatg ggaattacgc aggttggtgg ggcggcggag cgtataaaag
    17221 tgcgctgcat gactccaccg tttcatcgaa ttcctcattc gcgaacggcg gcggtgccta
    17281 cttctgctcg ctctccaact gtacagtcac cggcaattcc tccgagggtg ggggtggaac
    17341 cttgggcgga gcgctctaca actgcatact ttccaggaac tatgccgatg tcggtggtgg
    17401 atcgttcggg ggggagttgt ataattgttc actgaccgat aactccgcta actggggcgg
    17461 tggatcatcg caaggtgaac tgaacaactg caccctcttc gcaaactgtg cgcgattggg
    17521 cggtggtgga actgagtcgg gccggcttcg aaactgcatt atctggttca actccgcagc
    17581 tagcggggcc aactggaatg gaggcacact caactactgt tgcaccatgc cgatcccgcc
    17641 aggcgagggc aacctaggct gcgatcccct gcttgccagc gcctggcgcc ttggtggcgc
    17701 atcgccgtgt atcggccgag gtagcgctgc gtatgccagc ggtgtggata tagatggtga
    17761 atcatgggct actcctccct ccatgggttg tgatgaacga tccggctccg tgacaggcga
    17821 tatatccgtc gccatcatgg caaaatatac aaacttcgcg gtggggttcc cggttgaatt
    17881 cactgcgcag atagaaggcc atcccgcaga gagttggtgg attcttggga tggacggtgt
    17941 ggtcagcaat agacccataa ttaaccactc gtgggcttcg accggaacgt atgtggtaat
    18001 cctgaaggcg atcaacgaaa gttatcctgc tggcaattcg gcttcggcaa cggtcaacgt
    18061 ggtgcttgcg cctgtacact atgtcaacca gtcgaatact ttccccgact cccccttcac
    18121 atcatggcac acagcagcga ccaccattca agaggcggtc gatgcagcca cggttccagg
    18181 ggcaaccatt ctggtgacca acgggcttta tgataccggt ggtcgcgtaa taaacggaga
    18241 catgaccaac cgggttgtca tcgataaacc ggtcgttgtg cgaagcgtga acggaccgtt
    18301 ggtaacgttc atccggggga ggggcccgat cggcaatgga gcggtgcgct gtgcgtacgt
    18361 cggccgaaac gctgaattgg cggggttctc cctgacaggt ggttgcacaa tggcaatgac
    18421 ggatgaacgc acggagcgat gcggcggggg cgtgtggtgc gagagtcagg ccgtggtgtc
    18481 caattgcgtg ataacgggca attcggcttc ttcggcgggt ggtggggtgg cgcagggatc
    18541 tttgttcaat tgcactgttt ctggaaattc cgcgagcggc gccggcggca cctacgttag
    18601 ctttctccat agctgcattc tttcgaacaa ctccgccacc ggcgaagcag ggggcgcata
    18661 tcaaagcatg ctcgacggct gcattctcgt tgataacgcg gcccgcgaag ggggtggtgt
    18721 tatacaaagt atcctgttca actgcacact gttgggcaat tcggccacgg actcgggtgg
    18781 tggggcattc caaagcactc tcttcaactg cacggttgtg ggcaatcgag ccgcaagcgc
    18841 aggcgggacg cttcagggct cggcgaacaa ttgcatcata tttaacaaca ctggcggaaa
    18901 ctatgtgcag ggaactattc aatacagttg tacaacgccg atgccgtctg ggacgggaaa
    18961 catcacggat accccccatt tcagagcatc aaatgatttt catctggctg caaattctcc
    19021 gtgtgtcgat cggggccaga accagcagtg gatggttggg gcctgtgatt tagatggcag
    19081 cccgcgcagt gtcaatggaa cagtggacat gggggcgtat gaatttcatt tcgacggaaa
    19141 tctgaaggcg gttctccagg ggccgtatga cacgaatgcc cacttgatgg cgacattgtt
    19201 gagcagcaat gttccaccaa cttctcctta tgtggccgat gcgcggacag ttggaaacat
    19261 accgtccaat gtcacggatt ggattatgtt gcaggttcgg ccgggcatta ccaataatcc
    19321 tgtttacgct cgatcgctgt tcctccgaaa tgatggcagg attctttcgg atcagggcga
    19381 tacaaatcta ctgatcgagg tctcgccgct gacgaccaat tacttggtcg tctcccaccg
    19441 caaccatctt tccgccatgt ccgcccaacc cattattttc actaaccagc aaatctcgtt
    19501 cgatttcacg agcagttcgg accagtatta tgccggaact aatggcgcgg ttgaactgga
    19561 gccgggcgcg tgggggatga tcgcgggcga tgcggatggg gatgggaaga tcacggcggt
    19621 ggacaaggca atgtgcgagg ggcaggaagg acagacgggt tatctggccg gggatttcaa
    19681 tctggatggg tttgtgagca caaacgagcc gtgagctagg tcggaagagg ttgcggagat
    19741 ggccttgatt ggccacatgc gggtgccgga ttcatgtgtc gatttgacat ggctgtgaat
    19801 acaatgagcc cagtcctgcg agggggatgt atgaaaaaag tactggcagc tttagcgctt
    19861 ctttgctggg gggccggcct ccagggccgg gctatttcaa acgacctgta cttcgtcacc
    19921 aacaacatgg gccgagtcac ctccgtcccc aacggcgagg ttattcttta tccgcaactg
    19981 gtggtcagtc ctccacgaaa gacattggta gcgcaaagca cacaggtgat gaccgcacaa
    20041 ggttcaacaa atcccatcac ctggttcatg atcgagcggc cgagcggcgg aacgattacg
    20101 gtgaccagcc ccacgtccgt gacgtaccag gcgggcgcga cttccgattg cgtggacatc
    20161 gtcgaggcct gggacggcgg gaatatgttc ggtcgcgcgt acttgaacgt gatcagcccg
    20221 gcggaagtgg cggcggcggg caaggcgatc atcgtggcgg gccgcaaagg cgccgatgat
    20281 ccgttgtggc cggcgacgga ctacctggcc aacaatgcgt tcaatacgtt gctgtaccgg
    20341 ggctacggga aggagcatgt gcagtacctc aacccggtcc ctgggcgcga cgtggacggc
    20401 aatggaacgg ccgacgatat tgatcttgaa tcgaccctcg gcaacgcgcg cctgaccttc
    20461 accaattggg ctggcaatac gcatagactc tttgtctacc ttgtggatca cggcgaggcg
    20521 gcctccacca acgggtactt ccggctcaat gccggggaga cgctgccggg cacaacgctg
    20581 aaaggctggc tggacgacat ccagaacgcc tacaccacgg aagtcacggt ggtggtggat
    20641 tgctgctatg ccgggaccct gatcaaccag ttgagctaca cgggggtcgc caggcggatt
    20701 ctgatcgcct cctccgggac caacgagccg gcgtatttca tcgggggcgg gctggtgagt
    20761 ttttccgacg ccttcttcgg cggcatcatg ctcggctacg acgtgctgcg ggcctacgaa
    20821 caggcgcgca gtgccatgct gacctaccag ggcccggcct acacggacaa cgggagcgga
    20881 accgagggaa ccaatgtctg gctgggggcg acgttcgtgg cggggaagga tattccgcag
    20941 atcgggctgg tgtgcggccg ccagctgctg atggacggga cgacggcaaa cctgtgggcg
    21001 cgcgatgtcg tgtcgtccta tccgatcgag cgggtctggt gcatggtggt gccgcccagc
    21061 cacaatccga ccaacgcggc ggatccggtg caggacatac cggaactcgc cctcgtctac
    21121 accaacgacc ccgggcgtta ccaccaggac tacaacgggt tcagcgaaga gggaacctat
    21181 aagatcatct actacgccaa ggacatctgg ggcagcgtgt ccctgccgcg gcagagctac
    21241 gtggaccagg cgagttacga cgagcgggtg ctcctggtgg ccggcgacgc caccaacgcg
    21301 gccgacgggg cgtccatccg gaacatggcg aacgatgcct ggcacagcct gctggaacgg
    21361 ggtttcgcga cgaaccggct gcgttacttg agccacgaag cggcttcgca ggtcgccacc
    21421 tcgccctccc tctccgcgat ctcctatgcc attaccaact gggcgggcac gaccaactgg
    21481 ggcggaccgg cggataggct gaccgtctac atcgtgggac agggcgccaa cagccaactg
    21541 cgcctgaact cgggggagtg gttggcggcc tcccagttgg atcaatcgtt ggacctgttc
    21601 caggcctcca accggaccgt gaatgtgatc atggactttg ccgggtcggg ggcgtggatt
    21661 ccgcaattgg cgccgccggt ggagcgggag cggatcaatc tggccagcac gcgggccggc
    21721 gcgccgtgcc tgcgagaagg cgcgggcgcc ttgagctttt cgcgcttctt ctggcgggcg
    21781 gtgttccagg gcagcagcat cggaacggcg ttcgaccagg cgcggcgggc ggtctggatg
    21841 gcctcgggtt catgggggca gacggcccag ctggacgaca acggcaatgg cctggcgaat
    21901 gaaaaatccc aggaccggtt gatcgcccag ctgcgataca tcgggccggc tttccgaacg
    21961 ggcaatgatg cgcccgtcat tggcgccgtg acgccggaca cggaattgac cgggaccaac
    22021 cggttcatgc tgtgggcgca ggacgtggtg gacgcggacg gcgtctcgaa cgtctggtgt
    22081 gtgatcaccg aacccggata caacggccgc accggcgtcg tggaggtggt cctggcgtgg
    22141 aacgctggaa cgcagcgata cgagacggat tacagcggct tttccgcgac aggggtatac
    22201 gcctgcacct tctacgccgg ggatcggggc ggcgaaatct cgtcgcctcg gcaggccgtg
    22261 atccgcacgc gcgaggggaa tggcgtggac gccgacgaat atgaaacgga cgacctgccg
    22321 gaacaggcga agcccctgct cgtcggcgag acccagacgc gaacgcttca cgtgtccaac
    22381 gatgtggact gggccgtgtt tttcgtggga gaccagacca actacttcca cacgatccgg
    22441 acggatcatt tgaccaacac ggtggacacg gtgctggata tttaccagga gggcgatgac
    22501 ggggccttga ccttgatcca gcacgtggac cagtgggacc cggaccaggg cgagtcctgg
    22561 ggatggtatc cgagcaatgg ggtctacttc gccagggtga gctcaacgaa tttcacggaa
    22621 cccggaggct atgaactggc cttgacgata gatatcgggg ggcccttcga ccatctgctg
    22681 gtcctggcga tgaacggcct gacggcgggg gccgtgccga ccggggtcaa ggcgcatatg
    22741 gcgggttcag acctgccatt caatggggcc tattccgttg atttcgtggg tcccgacaac
    22801 gggacgcaca cggtgacgct gaccggagtt cctgcgggct ggtactatgt acagggctac
    22861 ccggcggccc agaacgtcac cgtgcccaac acgccgggca aggcgtacta cgcgcaattc
    22921 gtgctcctgc cgtgctttgg cataacggga acggtgcgag acgcgtggac cggggaatgg
    22981 ctggatggag cggccctgtc gttccaggcg accagcggac ggattgctgg acagaccagc
    23041 gcgagcaact ggtattcgca ggtcggcgga gggctccccg gcggggtgcg cgtggccacg
    23101 gtcaactggg ccgtgacgct ggccaaggcg ggctactcca atgccgtgaa gagcattgcg
    23161 ggagctggta tcgcggcggg caattctaca aactggggga ccgtgtggat ggtccccgtg
    23221 gacagttcgg gcgccggttt gccggatgcc tggcgcgatt tgtatttcac cggggccgtg
    23281 gatcccggcg gcgatgccga cggcgacggc caatccaatg aggaagaata ttgggctaac
    23341 acccatccga ccaacggcgc cagtttgttc atgtactatc aggcgcccgc ggcggcggcg
    23401 acaggcttca cgctcacgtg gccggccgcc tccggccggg actacgccgt ggagcgagcc
    23461 gccaccctgg tgccgggcga ctgggtccgc gtccatggcc cgcagacggc gtcggccggc
    23521 cagaccacca tgtcatggac ggacaccaac gcggttcagg gctgctgcta ccgcattgtg
    23581 ctgtatcctc ccgcccccta ggtgggctaa tgccatgggc atcatgaaac ggcacggctg
    23641 ggcagggcgc gctccggtgg ccacgtggcg aaaagctatc catcttgcgg cgcttctagt
    23701 cggtgtgacg ggcaggatcg ggcgcggtca ggatgccgat ggggtggccc tggacgtgac
    23761 acagcttaaa acactggccc agcagcacga gcaggccggg gaaaagcagg ccgccgcgga
    23821 tatctacgaa atcattatgg ccagggagcc ctcctcgcgg cagatcctgg cggcccgcct
    23881 ggccatcctt tgtgcggaaa tgggcgcgac caacaaggcg ctcgcttggg cccacgaggt
    23941 cgcctccgcc aatcccgacc cggaagctta tctcgccggg atttatgaga agcttggcga
    24001 tctgacgaaa gcccaggcga ttttaaagga tcttgttcaa caagaagcgg gctccagggt
    24061 gatgttggct cgtttctggc aactggctgc tgtttatgaa aagatgggtc agttggaatc
    24121 ttcagaagcc atgctgaagc gtgcttacga agcggcttcc agtccagcgg agaaagactg
    24181 cgcagtggct cgttggaatc aattcaagca gcgtcgagaa accagcctgg atgtagcttc
    24241 acccgtggat cccgctgtgc cgtcgggatc gtcgccaatg aagtagtctg gacagtcgcc
    24301 agcccaacaa ccaagccttt tggtttcagc gcggcatggt tctgcttcgc attaacaccc
    24361 cgaatagaga aggcgcgccc tccttgcgcc gccgattccc gaactggccg tttgtcgctc
    24421 gccatgaaag cccgcgtaac ctgttcgtat acagcaggta atgctcgcgc atcatttgca
    24481 aattattttt gaaagaaagg cgttttgcat ccgactagct aggcagaaaa ggagaagaac
    24541 aatgaaaagt gcaaatctac tcgcaaggac ttgggcaagc atcaccacga caatatcaat
    24601 ggtcaagggc gggatttcgg tgttccttat ctcgcacccg caatgggctc aactaagtcc
    24661 ttctttgatt gttgaggcgt cacccctgag gcgcccagac ctattttgtc gagttggaaa
    24721 agcgagccgg ccgcgttcga ttgtgatgct gaggacgatt atgattgccg gcctgggact
    24781 cccccatata gcactggccc agaaagctgc atccgcgcac ggcgaagcat ttgcctcggc
    24841 gacggtgatg gaacagattg ctgcaatggg gcgagagaag aactcttgga ctcccgtcca
    24901 gcggaaaatt agttcgaagc ttcttcttga gacaagacaa cggcttggac ggccaatcac
    24961 tgcaggtttg cccgagatac gctctgccgt ggaggtcgac gccgcaggac ttacacttgt
    25021 ggacatcaag gccgaagtga gtgatgggct gctgcgtcgc attcaacacc ttggtggtca
    25081 agtgattaac agccatgtgc gatacagctc aattcgcgct ctcgtcccca ttgcgcaact
    25141 ggagatcttg gcgggagaga cagccgtcaa atccattaag ccatctgata aactgacaac
    25201 ccacaaagtc gacacttcac agggagtggt gacgcattgt gccgatagtg cgcgtacaag
    25261 ctacggcgtt gatggtacgg gtgtccgcgt tggcatcctc tctgacagtg tggaatctct
    25321 cgcctccctt caggcatccg gcgatcttcc tgcaggggta accgtacttt cagggcaggc
    25381 cggtagcggt cgtagcgagg ggacggcgat gttggagatc gtgcacgata tcgctcctgg
    25441 tgcaggcctc tatttcgcca cggcaaacgg cggacaggca cagtttgcgc agaacatact
    25501 ggccttgcag gccgttgggt gtaaggtgat cgtggatgac tgtagctact acgaagaacc
    25561 agtctttcaa gacgggatca ttgcgcaggc cgttgaaata gtcactgccg cgggctgcat
    25621 ctacttctcc gcggcgggca acgagggcaa cctaaacgac ggaacatccg gcgtgtggga
    25681 aggcgatttc gtggcagcag ccggccgtcc gacgccaatt acaagtggca ccgctcacga
    25741 tttcggtgct gggatcaact acaacactat cacagttgat tccccttctt tcttcaactt
    25801 gaaatgggcg gacgctttgg gggcatccgc gaatgactac gacctatatc ttctcaactc
    25861 aacacgcacg acaatatacg attattctgt cgatttgcag gatggcaatg atgaccctta
    25921 cgagtctatc gacagcttca aattcaatga tacaggcaac accctggtaa ttatccagaa
    25981 gaccggcggt aaaacgcgtt tcctgcatct gagcgcaaac agagggcgct tctcccggtc
    26041 gacggcgggg caaatcggcg ggcattcggc tgctgcatca gccttgagcg tcgctgccgt
    26101 agatgtcgcc acagcgggcg gaggggtttt cgttggaggc gcggcaaacc cagtggagag
    26161 gtttagctct gacggaccca ggcggatatt ttacatggct gacggaactc caattacgcc
    26221 cggtgatttc tcctcgacag ggggagcgct gcgccagaaa ccagacatcg ctgcggcaga
    26281 cggtgtgagt tgttccactc cgggattcaa tctgtttaag ggaacctctg cggctgctcc
    26341 acatgctgcc gggattgctg cgctcatgat cgacagcggg ttgaccacgc cggcgcagat
    26401 caggcaggca atgacgagtt cagcctggga tattgaaacg gacggtgttg accgtgattc
    26461 aggctggggg cttgtcaacg cgctggctgc gcttggatat tccattgccg agaatgtcag
    26521 cgctccaacc acccctgcgg ggctgtctgc cggattgacc aatcagccgc tggtgttttc
    26581 ggcgggcggg gcgaagagca gcgtcgggca cgccgtgcag tatcgtttcg gatggggcga
    26641 cgggaccttg tcgccctgga cagcggggac ggccaccggg aagtggagcg cggccgggtc
    26701 ctatagcgtt gtctcccagg cccgctgcat cagcggccac gcgacgtcgg cctggtccgc
    26761 ggcccggctt gtcgccatca ccaatacgcc ggcgggcgtg ctcggcgcgg cggcggcgtg
    26821 gatggaggtc ccggcaacca tgatttccgg ccggacctac cgcgtgaaag tggccatgca
    26881 gaacacggga acgatcccat ggaccctggc gggcgggttc aagctgggat ccgaaatggc
    26941 ggttgatact taccggtggg gcctgacgcg ggttcaagtc accaacacgg tggcttcggg
    27001 ggccgtgcag gtctttgaat tcgatgttac gccccgcgaa gtgggcggcc ggccttgcgc
    27061 gtggaggatg gtgaaagagg gctaccgttg gttcggggcc accaacagct cgatcgttgt
    27121 ggtctccgcg gccgcgtctc ccctcatgcg gaaaatcaat tgcgggggcc cggcggtggc
    27181 gggaggatgg ctggccgata ccggttgtct ggcggggcag gcctatgtgg tgaccgctcc
    27241 catcgccaat gcgggagccg ttgctcaagc ggtctaccag tcttgccggt atggcccggg
    27301 tgcgaccccg ttcggctaca gttttcctga tgtccccaac ggcaactaca aggtgaagct
    27361 gcacttctcg gagcctctct ggacggcggc cggactgcgg ttgttccata tcgatgcgga
    27421 ggggcagcgg aaactgtcgg gcctggatgt cgtcaagcgg gccggcggaa ggaatcgcgc
    27481 gctggtgatt gaactggtga ttgccgtccg ggatggcaac gggctgcagc tgcaattcgc
    27541 ggcggtgaag gactgtgcga tggtcagcgg catcgaaatc gtgcaggaat ctgtgagcct
    27601 gccgcgaatc aactgcggcg gcgccgggat cggcgacttc gcggtagacc ggggcggcgt
    27661 cgggggtcag gtttaccagt ctgcggcggt gatagccggc gccggcgtta ctcctcaagc
    27721 gctctaccag acttgtcgat atgggcctgc cggcgccgcc ttcagctatg cttttgccga
    27781 cgtgccgaat ggcgcttaca aggtccggtt gcatttctcg gagcccgttt ggtggaaggc
    27841 gggacaacgc ctgttcgatg tggtgatcga aggagtgcgc aaggaagccg gcctggatgt
    27901 gtggtctcag gccggaggta tcaatcggtc cctggtgaag gaatatgcgg tcgcggtttc
    27961 agacggtaac ggcctgcaga tcgtcttcac caacgccacc agggactgcg ccatggtcag
    28021 cgggatcgag gtcatacaag cctcggccgc cgcggacgga ggcggcttgc ttgtccgcac
    28081 ggctgcgcca acggccttct ctgcgaacgg ggttgaccgt gcggcccccg gcccggatcc
    28141 gatgtcgttc tcaatcccgg ccccgctgga tgtcgtgacg agcggcaacg ccttcgaaga
    28201 cgcaacgggc tgggcggcgg ttgaccagtc ccatgaaaca gcctggatcg ggaatcccgg
    28261 cggcaacggg tggtgggtgg cgcttgcgta tgagccggag atcacggcga gccagctgga
    28321 gctgaccctg ggcgctcatt cgctcacgaa cgtgcagtgc ctgtgcagcg aagacgccga
    28381 tcattgggtc gatttccatt cggcggccac caatggcccg gtgacgcttc gctatctgtg
    28441 gctcgtgttt cctgccgatg ggtcggaccg agttcccgtg gtcctggaaa tcgcgccccg
    28501 tgtcgaacgg ggtgattgat ccttccgcgc cgctgcctgt gatgccggcg gggaggaggg
    28561 gggtgcaggt ccgcgaaagc ccgcggcctg catccctgca caggctgtat cccgccagcg
    28621 ctcgcgtaat atgttgtgcg caagcaggat gcattgcgat gactccagtg aaaaatagtg
    28681 aaagaaacgc gcttctgatc cgactgattg aatagaccat gaaaggagcc tccatgaact
    28741 tgaccaactg gccggcgatg tcgttcggaa tgcgtccgtc tcggaagatg gcctggggac
    28801 tgctcatgct ggtgtccgcg acggccaccg gcatccgggc ggctgaatac gtggtgccga
    28861 cgaatggcgc tgctgcctat ggaaacaccg gaacgagcta tccctgcctg tcctcggcgt
    28921 gccgccgata tcagcaggtg tatgccgcca gcgagtttgc gtcggcccag agcccgcagc
    28981 tcatcacccg gctcggattc cgctatcgca ccgcgacgag caccttttat ctgtccggca
    29041 cgtggatgga ggtgcggctg tccacgacga gtcgcaaccc cgacggattg agcgccacgt
    29101 tcgcggacaa tccgggcgcg gatgaaacgc tggtctatgc ggggaccaac tggcagttcg
    29161 ccgggtcgtc cgccctcggc aagttctgga tggatgccta tttcgatcgg cctttccact
    29221 acgacaggac gaagggaaac ctgctcgtgg agatccgtat gccgggctat tacaacttct
    29281 ccggctacac gctggacgcg accaacgcgg cgtccgattc cgtttcccgg ctgtacgcgt
    29341 ccgcgaccaa tgcggccgcc ggcaccctgg atagcgtcgg cgccatcacg gagttcacct
    29401 tccaggacgt ggttctttcc aacatcagcg cagaagctgt ggccggcggg atcgtcctcc
    29461 ggcacgcgag catcagcaac gccgagtacc gcgttcaggt gtcgaccaac ctggccgggg
    29521 ccttcgcgaa tgcctcggca atatacaccg cgagcccgcc tttcaatgtg gtcacgcaat
    29581 tcccggggag cgggctgaag ttctaccggg tggtccggga gaaatgagcg ggcccgggcg
    29641 gtcgcgagat cggccaggat tggagaataa tcgctacagg ggaaggaggt tcgccggcag
    29701 cattgaccgc gtgtggatgt aagcgctcgt gccgtgattc agggaattgt tgaaacaaaa
    29761 caaacacaag gagagatcca aatgaagaag atggcggcta tgttggtcgg tatgatgtgc
    29821 cttggagggg tcgcgcatgg cgagagtcgg cttggtccgt tcgcctcctg gctcacctcc
    29881 aacgacgaga atgcattcgg cgtgggtctg aagtacgaat ggctcttcaa cggaaagttc
    29941 gggatggatg cgcgtgccgg ctacctgacg gatagcgata tgtacgtcat tcccctggaa
    30001 ctgggcgtgg taggcatcct gccgctggag cgactgtctc tgtatgccgg ggtgggcggc
    30061 gggtactata tccccgaaga catcggcatg agcacgccgt gggggccggt ggacgggccc
    30121 gatgccacct tcggcttcta tggagtggcc gggatcagga tgccgacggg caagaacatg
    30181 gagttcttcg cggaagcgaa gtacaccagg gcggaaagcg atgaggagag cacgtcggga
    30241 cccatttacc gaaacggcca actcgtcggg tatacggtcc atcagaccac gaagatgggc
    30301 atggatctgg acgggatcgg cgccaacgcc ggcatcctct ggaagtttta gccgcgtata
    30361 agcgcctcga caccgtggtt catcctgcgc agccgcaggg tgaaccacgg tttgcatttc
    30421 cggcagggat ctgtgggcgt cgggccgtaa tcgtttccat cggcgcgtgt gcgagcggca
    30481 gtcctgcgcc tgcgatatct ccttgtcgtt gtccgttctg gtgctatgaa tacgcagcac
    30541 aggcgggccg gatcgaagga agcagcaacc ggttggacga aggatgagca cggcaagaag
    30601 cgggaggggg acgcggggct ggatcgggcc gatcctggcg atcgtcctga tcgtggcggc
    30661 cggcggcgcg tggtggtatt tcgaaaagcc ggcgaagccg ccggacttcg ggacgctggc
    30721 cggacgctgg gtcaggccgg acggcggtta cgtggtggag atcaaggcgg tggggaagga
    30781 cggcgcgctg gaggccgcgt acttcaaccc aaaacccatc cacgtggccg tggcccgcgc
    30841 cttcacggag gccggcgccc tgaacgtgtt catcgaactc cgcgacgaga attaccccgg
    30901 ctccacgtac acgctggcct acgacccggc ggccgaccag ttgcagggcc tctactacca
    30961 ggccgtcatg gggcagcgct tcgaggtcta ctttgaaagg atcaagtagg cgtggccgag
    31021 gttcccagga gggacgtatg gcatccggcg ggtcccggcc agccggtcga ggtgttcacg
    31081 tcgctcgacc ggtacgacgc gcccctgatg atggcggacc gcgggcgccg ccggttccat
    31141 gtgatgatca agcccgtcgg ctgccgctgc aacctgaact gcgcctactg cttctacctc
    31201 cacaaggccg acctgccggg cgggccgggc gccgggcgga tgtccgacgc ggtcctcgag
    31261 cgcttcatcc agcagtacat cgagggcgtg acggcggacg aggtcgtgtt ctcgtggcag
    31321 ggcggcgagc cgacactcct cggggtggag ttcttccgcc gggtggtcga actggagaag
    31381 aagcacgcgc gccccggcca gacgatccag aacgacctgc agaccaacgg cacgctgatc
    31441 accgaggagt ggtgcgcgtt cctcaaggcg aaccggttcc tcgtggggtt gagcatcgac
    31501 ggcccgaaga aactccacga ccggtaccgc gtggcgaaag acggggcgcc gacgttcgac
    31561 caggtgttcc gcgcggcccg gctgctggcg aagcacggga ttcccttcaa cacgctgacg
    31621 tgcgtccacc gggagaacgc ccgcttcccg ctggacgtgt atcgattcct gcgccgtgag
    31681 cttggatcca cctacctgca gttcatcccg atcgtcgagt acaagggctt cgagcgcacg
    31741 gccccgcaaa aatggaactg ggaacggatg ccccgggacg gcgaaccgga ggcccggccg
    31801 ggccacccgg attcggtcgt gaccgactgg tccgtggacc cggacgactg gggctacttc
    31861 ctctgccggg tgttcgacga gtggcggagc cgcgatctcg gcaaggtgct ggtcaatcac
    31921 ttcgagacgc tcgtggcgca gcacctgggc ctgccgtcgc agctctgcgt ctacggcgag
    31981 ttctgcggca agggcgtcgc ggtggagcag gacggcagcg tgtacgcctg cgaccactac
    32041 gtctacccgg agtaccggat cggccacgtg aacgagaagc cgctggccga cacggtgctg
    32101 tcgcgggcgc aggtgcagtt cggctacgcg aagaacgagg ccctgccgaa ccagtgccgg
    32161 cagtgcgaat ttctcaagga ctgctggggc gagtgcccga agaaccggat cctccgcacg
    32221 gcggacgacg agccggggct caactacctc tgccgggggt tcaagcggta cttcgcccac
    32281 gccgtgccgg aggcggagcg catcgccgac gggctgcgcc gccagccgcg ggagagccgg
    32341 cggcggatgt gacaggcccg cgaatccctg ttggcgcgcg gcctcccgcg gagtatgctg
    32401 accgccgacc gatgaagccg aactccatcc ggaaatcagg cggggccgcc atcacgatcc
    32461 tgctctgcct cctctgcctc ggcatcgggt tcgccgccgg atacctcgcg cggcgctggc
    32521 aggcctcggg gtcgtcggcc atgccggccc ggtgcctgcg cgtggtggac acggatacca
    32581 tcgtgatcca ttggctctac ggcaccaaca aggtccgcat ggcgggcatc gacgcccccg
    32641 agagcaagga gaacaagaag ctgcgggagc aggccgagat gctcggcatc aagcccgcgg
    32701 ccctgctcca ggtcagccag gtcgtcatcc gccagatgga tatcatgctg acggggaagg
    32761 acgtgaggat cgtgttcccc cgcggcaagg tggaatacga ttccttcggc cgcgtgctgg
    32821 cctacatcga ggtcggcggc aaggacatct gcgagatgct gatgcgcaat gggctggtgt
    32881 acccgcggcc ggagccgcat ccgcgcaagg aacgctatgc ccaggtcaac gcgcaggcca
    32941 aggcccagcg caagggcatc tacggcatgt ccggcgcgaa gtagcgccgc gacgcatcag
    33001 cccgcgtcca cgatcgaggg cgtgtagtcc gccggcggct cgtgccccag cagcgtcagg
    33061 cacgtggccg cgaggctgct gatgccgagc ccgccgccct gcgccgccgc ggacagccgg
    33121 acgcacgacg cgcccgacgg gtcgtagatg ccgcacggca ccgggttgag cgagtgcgcc
    33181 gtgcggatct tgggctggcc cgtgcgcggg tccatgacca ccgcgccggt cttcttctcg
    33241 tgctcgtaca tgtcgtccgc gttcccgtgg tccgccgtga ccacgaggat gccgccggcc
    33301 ttttccacgg ccttcatcac gcgcccgagg cacaggtcca cggtctcgac cgcgacctgc
    33361 acggcctggg gaatccccgt gtggcccacc atgtcgccgt tggcgtagtt cagccggatg
    33421 aagcggaact tgttgcgctt ccgctcgatg gcctcgatga ccttgtccgt gatctccgcc
    33481 gccttcatcc agggccgatg ctcgaacggg acgcggtcgg agggcacctc gacgtaggtc
    33541 tccagcttgt cgtcgaactt gccggagcgg ttgccgttga agaaataggt cacgtgcccg
    33601 aacttctgcg tctcgctgat cgccatctgc cgcacgcccg tggcggagag gtattcgccc
    33661 atcgtccggt cgatgccggg gggcggcacg aggaaccggt gggggatgcc cgtgtcgccg
    33721 tcgtactgca tcatgccggc gaagagcacg gccggccgcg ggccgcggtc gaacttgtcg
    33781 aaagcgtcct cctcgaaggc ccggctgatc tcgatggcgc ggtcgccgcg gaagttgtag
    33841 aagatcacgc cgtcgccgtc gcggatcggg ccgaccggcc ggccgtcccg gacgatcacg
    33901 aagggcggga gatcctggtc gatcacggac gggttggccg cgcggccggc ctcgatggcc
    33961 gcgcgcgcgg aggggaaggc cgggccggcg ccgcggacgt ggatgtccca gccccgcttc
    34021 accatttccc agtccgcctc gtagcggtcc atggtgatct tcatgcggcc gccgccggtg
    34081 gcgatcgcga agtccgtcgc gccgccggcg ttcagctcct tcaggaaggc ctccaaccgg
    34141 tccacgtaga ccagcgcgga ctgcggcggc acgtcgcgcc cgtcgagcag gatatgcacg
    34201 aaggccttct tcacgccggc cgcccgggcc tcgcggagca tggcctcgag gtggctgatg
    34261 tggctgtgca cgttgccgtc ggagaagagc ccgaggaagt gcagggcgga gcttttctgg
    34321 aggccgttct ggaccagttc ccgccacgcg gcgccctcga acagggagcg gctctcgatc
    34381 gccttgttga cgaggctggc gccctggtcg aagacccggc cgcagccgat ggcattgtgg
    34441 ccgatctcgc tgttgcccat atcctcgtcg ctgggcatgc cgacggcggt cccgtgcgcc
    34501 ttgagctggg tgaacgggac gtgggcccgg agccagtcca gatgcggcgt ggaggcggcg
    34561 cggacgaaat cgccttcctc gtaccggccg atgccgacgc cgtccatgat catcaggacc
    34621 accgggcctt gcgggccgcg gcagccggcg agcggtttca acgtgtccat ggctgattct
    34681 ccgggttcgc gcggccggac atccgcgccg cgccttcagg gccgctcaat atgccccgtc
    34741 gggcgggccc gcgcaagggc ggaatcaggg gccggttgac ggcctccgga ttgtcgggta
    34801 gcttggcggc atgagtgcgg aagcgtctgg agcgggggag ccgcggatcg aggagcacct
    34861 ggaggccgtc cggtcgcgca tcatgcaggt catgacggcg acggcggtcg gcccgctggt
    34921 ggccggctac cggcgcctgc tggtccaggg caagatgctg cgcagccgcc tcgcctggcg
    34981 cgtgggcgag gccctgcagg ccgacccggc gatgctggtg gacgccggcg ccgcgataga
    35041 aatgatccat gccgccagcc tgctgcacga cgacgtgatc gacgggggct acctgcgccg
    35101 gggcctgccg gcgttctggg tggagcaggg gatcccgggc gcgatcctgc tcggcgacct
    35161 catgctgttc caggccttgc ggctcctctc ggcgcccggg cagcgcgccc tgctcgggga
    35221 ggccatccgt ctcaccggcg aagtctgcga ggcggaatcc gagcaggagc tgatgatgcg
    35281 cgaagtcccg tccacgtggg aagcgggcgt ggacgtcgcg cggcggaaga cgggcgcgct
    35341 cttcgccctg gcggccggcg ccggcgccgg cccggagacg ccttccctcg acgcgctccg
    35401 ggaaagcggc ttcctggtcg ggaccgcgta ccagctggcg gacgatttcc tggatgcgca
    35461 cggggacgcg gagacggccg ggaaaaccct cggcagcgac gcgggccgga acaaggccac
    35521 cgcgttccgc gcggccgccg aggccggcat cgacgtgccg cgctacctcg aggccctgct
    35581 ggcgcaatcc gtcgacgggc tggcccggtg gccgaccgtg cgggcggcct gggcccgata
    35641 cctggaaacc gacttgcggc ccgcgctgga ccataacctg cgctgccagg ccgcgttgtg
    35701 agccccgcgg gccgccggcc ggcaggtcga agaaggcttg atcaaagggc cgattccggg
    35761 tatagatagt tctttggaga agtcgtatgc gctggattcg cgggaataga tgggcggtcg
    35821 cggtcgtcct gctcgccgcg ctggtcgtgg ccgggacgct tgcgcgcgtg gcgtaccggg
    35881 cgacgcgggt ctggtccgtc ggcgccgatt tccaggagcg cctgcgctac ctcgtggaga
    35941 ccgggcagac catcggcgag aagccggcct ccgaaacgtt ctccgtcgac tacctccaga
    36001 cgatcttcgg cgacaacccc gaactgctcg agcacctgaa gtcggtcgtg cagcagggca
    36061 tggcccagga ctcgaccctg accgccggcg aggtcacggc cctgctggtg acctatcacc
    36121 tgcaggacga cggcagcgtg gccgacgtga tcgtgcacgc cgtgggcggg ttcccggcgg
    36181 cgcgcaagcg gcccggcttc caccgccacg gctacttctt ccagcagatt gacaacgacc
    36241 tctggaccct gggcaacatc gccatcgggt tcctgggccg cgacatcatc gtgttcggcg
    36301 ccgacgaggc cgccacggag cggcagcgcc agatcctgga ttccatcttc tccggcgaga
    36361 tcatgatgct cgtggcccat ctccaggagc ccctgtactt cagcctcgtc ctgccggacc
    36421 cgcgccaggc cgtgccgccg caactgcgca accacgtgca ggccgtcgtg gtcaaggggt
    36481 acctcggcca caacgacggc cggtgcgaca tcctcgtgct gaccccctcg ccgcgctcct
    36541 cgcggtacgc cctcaacctg ttcgcggaca tgaagacggc ctccgaggcc gccctgcgca
    36601 gcaagtgggg cggcgtgaag aagatgaacg agtggggcat gcccgagtac gactggtggg
    36661 cgttcgagat ggtcaagacg ctcgaacagg ctaccctgga gcaggagttc agcatcatcc
    36721 gcatgggcac cacgttcgac cgggtgatga tcaacgcgat cctgaaatcc atcgagcgca
    36781 tgagccgcga cctggcggcc atgcagggta tcgaggacga gcgcctcgac ccccgcctgg
    36841 tcgatgcccg cttgaggaac tccaatccgg aggtccccac gacctacccg ctgcattact
    36901 ggagcgcgca gcaccagtgg gggccggact ggcccatccc ggccaccacc aacctgcctc
    36961 cgctcacatc ctcttccgag actccggccc ccgcggaaag cccggttccg gcggaacccg
    37021 ccgcgggcac ggaagttccg gccagcccgg ccgcccctgc aacgccggca acgccttgac
    37081 caagccggtg tgatgccgag tcacacgtct tacccggttc cggccgattt ggctgcgctt
    37141 tccctattct aaggattgca ctatttccgc ggctgcacta gtatcaagca cttttatacg
    37201 tgtgcccgtc cgcgaatatg ccctttgcgg aacggggttt gcgtattttg gcatgagctt
    37261 gcagcgcata gaacgctgtt gtcgcagcgc agcggggggg gtgttgcttt cgctggcaac
    37321 ctggacaccc ctcgcgtggg cggcgcccgc ggagtacacg gtcaagcagg tgtcggacga
    37381 ccatttcttc agccgcgatc ccgtcatcag cgacacgggc ttcgcggcct ggtcggccta
    37441 ccattccgag gacggcgagg acagcgcgtc ggatatccac gtctacgata acggccagca
    37501 gcgctcgctc acggggggca atgtgcccgt cggcacgacg cattatcggc cctgcgtgta
    37561 cagcaacacc gcggcctgga tcggcagttt cccctatccg aagacgggcg aggggaactg
    37621 ggtcctgaag gaagtgccgg ccgaggtgcg cgatgcgccg tacgaggagc tggtcgcctt
    37681 cccggagtac atgaccacca acgccgtgcc gacggagccc gtgacgaatg ccccggcggc
    37741 ggagacgccg ggagagcctc cgcccggcgc gccggcggac gaggcggccg cggaggaggt
    37801 cggcggccct gattccgccc gccggcatcc cagcggcgtc atggaatcgg tggcctggtt
    37861 cggggacgag atcaagcgca tcagctacga ctggcgcaac gatttcggcg tgagcctgtg
    37921 ggggcggacc ctggcctggc agaagtcgaa gggcttcccg ttcggctggg aaatcatggt
    37981 gtggaacgac gggaccatgt accagctgac caccaacttc ttctacgaca tggccccgca
    38041 ggtccaggga acccagatcg cctggtacgg atgggacgga aacgactacg agatttacct
    38101 gcacgacttt gcggccggca ccacgatggc gatcacgagc aaccgctacg acgacgtttc
    38161 gccggtgctc tgggacggtc aggtggcctg ggaggcctac ccgggcgccg acgccgaagt
    38221 gtacctttgg aaagagggcc gcttccagcg gttgagcgac aatccgtggg acgacaccaa
    38281 cccgcgcatc tggaacggca aggtggtctg gcagggcttc gacggagacg atttcgagat
    38341 ctacctgttc gacggcgtga agtccgtgaa gctcaccagc aaccgccacg acgacgtcaa
    38401 ccccgacatc cgcgacgggc tcgtctgctg gatggcctat tatgacaact gggactccga
    38461 gatcttcgcg tgggacctcg acaagggcga gtacatcgcc atgatcaacg aggagggcca
    38521 ggtcggctcg acgccgggcc gcttgagcta taacgaggtc gaggaccgcg agccgcgcac
    38581 ggcgggccgc cgcgtcatct ggcaggtgca gcaggagggg aaatccctca tcatgctcgc
    38641 cgagcccaag gctccgtgac ctgatcagac ccagtaccgg cggtccagcg agcggtactg
    38701 gatcgcctcc gacacgtgcg gcgtgcgcag cgtctccgac ccgtccaggt ccgcgatcgt
    38761 gcgcgccacc ttaaggatcc ggtcgtacgt ccgcgcgctg aaatgcagtt ccgtcagcgc
    38821 catcttcagc aggtgctggg cctcgtcctc gatcgggcag tgcttgtgga tgtcgcgcga
    38881 gcgcatcatc gcgttgcaat aaacccgcct ggcgccctcg aaccgcttcc gctggagctc
    38941 ccgcgcgcac aggatgcgac cgcggatcgc ggaggagggc tcgccgggct cggcccgcga
    39001 cagctccgtg tacgccaccg cggggacctc gacgtggatg tcgatccggt ccagcagcgg
    39061 gccggagatc cggtgccggt agcgctcgat ctggatcggg ctgcagcggc actccttccg
    39121 cgtgtccccg aagtatccgc acgggcaggg attcatcgcg gccacgagca tgaaccggga
    39181 gggaaaggtc acgctcgccg ccgcccgcga gatcgtgacg tgcccgtcct cgatcggctg
    39241 gcgcaggacc tccaggacgt tgcggtggaa ctcgggcaat tcatccagga acagcacgcc
    39301 gttgtgggcc aggctgacct cgccgggcat gggatgcgat ccgccgccca gcaggccggc
    39361 gtccgagacc gtgtggtgcg gggcgcggaa cgggcgcagg gccaccagcg cctggtgcgc
    39421 cgccaggatg ccggcgatgc tgtgaatctt ggtcgtctcc agggcctcct ccagcgtcat
    39481 gggcgggagg atcgacggga cgcgccgcgc cagcatggtc ttgcctgtcc ccggcgggcc
    39541 cagcgtcagg atgttgtgcc ccccggccac ggccacctcg aacgcgcgct tggcggactc
    39601 ctggcccttg acgtcggcga agtcctcgtc gccgtgcgac gcctggtcga acaccgcgcg
    39661 cgcgtccagg cggtagggct ccagggccag cgtcccggcc aggaaatccg ccgcctgccg
    39721 gagcgtccgc acgggataca cgggcagccc ttccacgacg gcagcctcct cggcgttgtc
    39781 ctccgggatc atcagccccc ggcacccggc cgcgcgggcc tccatggcga tgggcagcac
    39841 gccgcggacg cggcggatct ctccgctcaa ggccagttcg ccgagcacgc acaggccgtc
    39901 cagccggccg ggcgcgagtt cctcctccgc gaccagcatg cccagggcga tgggcaggtc
    39961 gaagctgggc ccttccttct tgatatccgc cggggcgagg ttgatcgtgg tgcgcccgcg
    40021 cggcggcatg aagccggagt tgatcagcgc ggtgtggacg cggtccttgc tctccttgac
    40081 ggcggcgtcg ggcaggccga cgacgatcgt cgccgggtcg ccccggcccg cgtggacctc
    40141 gatctccacc gcgaaggcgt ccacgcccag caccgccccg gatctgaccc tggccagcat
    40201 gcgcgtcgcc ccctcaatcc ggcgtggcgg gcggcggctc ggcgcccagg ttccgcagcc
    40261 aggtccgcaa gtcccggttg gtctcgagga gggaatcgta ggggtgtccg cccgcggccc
    40321 aggcggccac ctggcgcgcc tgctcggggg gcagggcgcg cgccgtgtcg cgcgcgtcgg
    40381 taaagcgccg ggcggcgctg taggcgcaga tcagcgcgaa ggcggtctcc gcgtccgagt
    40441 cggccatctt ccgggcttcg acgagcgcct ccacggcctt gtcatattcg cgctcctcca
    40501 gcagcaccag cccgtatatc cgctgcgcgc cgggccactc cggacgttgc gcgatgagtt
    40561 gctccagttc ctcgatggcc cgggtttttt cgcccaggcg gtacagggcc agggcatggt
    40621 cccgcgtcgc gtccggggat tccaggccgg cctccctggc cgcttccagg ccgtcccgca
    40681 cggccgccca ctgccggttg gtcagggcct ccatcgcgcc ggccagcgcc acggcgacgg
    40741 agcccatccg cgccttccat tcctccaccg tcggctcgga gcccgattcg gccggcccgg
    40801 ccgacctccg gcccagcgcc agctggcaca gggcctcgag cggacggtac ccgaacttgc
    40861 cggccagcag gtcggcatcg gtttggatcc aggtcgccat ctgccggacc tcgtcgagcg
    40921 gcaggccctc aagcagcggc tcggccaggt cgcgccgtcc gttcatcagg tggatgctgg
    40981 ccagcaggta gcgcgacgac agctgggcgg gatcgagttc aatggcccgc gccagcgcga
    41041 cctgggcgcg gtcgaagcgg tccagttcaa agaacacgtt tccccagcgg gcaaacaccc
    41101 acgcgttgga gggatacagc tcggacagcc gcgcccagta gctctccgac atcgcgtact
    41161 ggccgcgctt cgtgtaaagg tccgccgccc accacaggag cgtcgggtgg tccggcgcca
    41221 ggctcaaggc ctgctggacc tccaggctgg ccgcgtcgag atccttgtgt tccagcaggt
    41281 actgcgtctc ctcggcatga aactgcgcca gttcgttcag gttggtccag tcggtgggaa
    41341 agtccggcgg ccggcccggt tcggccgccg ccgaaagccc ggcccagacc gccagccaga
    41401 ccgccaggat ctgattccca gggaatctca tgttctcagc gcgccttttt aacacggagc
    41461 cgccgggccc gcgagcctat cttttccggc attgcgcagg cccgccccgc cgggcatgat
    41521 aaccgttgaa taggccgggc gccccggcgt ctaaaggtgt tggaggacag aaccatgaag
    41581 aaacatgcga ttgcagccgt gctgatgatg atccccgccc tggcgggctg ggcggacccc
    41641 gtgaaggtgg ccatcctgga tttcgaggac cgaaccgggc aggcctccga cgcgctcctc
    41701 ggcggcgcca tcaagtccgg cgccctggcc gacaagggcc tcgacctgct gggccgccgc
    41761 ctgctcgacg ccgagacctt cgtgctcgtg gaccggcgcg acttcatcaa ccagatcgag
    41821 cagctccagc ccaccgacag cggcgcgccg acgcccacga agccgtcctt cctgcatgcc
    41881 gcccaggccc tgaacatgga cgtggtcctg cgcggcagcc tgttgagctt ctcgcccggc
    41941 aagcagaccg tccggcaggg cggctacgag acggaattcg tcacgctctc cctgcggatc
    42001 agcgtcgagg ccgtggatcc cgtcgacggc acggtcatcg ccatggccga cggcgccgcg
    42061 gccggccagt tccgccagac cgccgagcat ttcacctccc tttcggagga cgacgccctg
    42121 ggcctgctgg acaaggccgt cgaccaggtg gtcccggtgc tcaaggccaa gctcgatcag
    42181 cgccaggccg cgcaggccgc ccgcccgcgc gtgaagatca gcgtcaagag ctcggccgac
    42241 ccggccctgg ttgagatcga cggcatcctc gtcggcagca cgccgctgga gaacttcgag
    42301 gtctaccagg gcgaccacgt cctgaccgtc ggcaagcccg gccaccgcga catcaccaag
    42361 cgcatcctgc tggagcagga ctccgcgatc gaggttccga tgatgcgcac cgagatgtcc
    42421 gccgaggagt ggaaggacat cctcgagaag gtgcggatga acgtcgtcat cggcgagccg
    42481 gggatcgtca tcaccccgct ggcggaataa cgcccgcgcg ccgggacgtt tgcggccggt
    42541 tttccaatgg ttggaaaacc ggccgctgtt ttttccaatg tctggaaaaa ccgccggcgc
    42601 ggcccggagg cgcgccgggg tcagggcgtg acctcgccca gccgcgtgcg gaataccgcg
    42661 ccgctcacgg cctggaagcc cggcccgagg gtgatcttgt tccccgcgtt cagcgtcacg
    42721 tcgcccggcg actgcacgcg gaactggtcg aaggcggtga tcgtgttgaa ggcccggtac
    42781 gtgcgggtgt cggtgacgac cgggttggtg aggacgaggt cccacgggat gcccgccact
    42841 gcggcccacg cgttcaggcg gccgtagccg tagtactcgt tccagccgtt ctcgtcgtag
    42901 gggacgggtc cgatccgctc gcaggaggcg cgcatccggt cccgcacctc ctgccaggtc
    42961 aggttcgtgt cggtcgagag gatcagcgcc gcgatccccg ccgcgagggg gcaagccgcc
    43021 gaagtgccgc cgaacgtcgc cgtgtagtcg ccatccgacc cttccgccgt gttgtagccg
    43081 ttggtcgagg tgcgatcgat cgtgacgatc tggtgggtgt cttccaaggg gctcaaatgc
    43141 ccgcccgaac tgggcacgac aaactccagg cccgtgccgt actggctgta gtctgaccgg
    43201 tagtcgaagt ccgtgctggc cccgacggcg atcacgttag agtgcgaagc cgggtagtgg
    43261 atgtagtagt cgatttcatg gtcgccgggc aaccacatgc gatctcccgg gatgcccgag
    43321 gtgatgtatt cgatgttggt gttgcccgtg ttcaggtaat gccattcgac cgtcagccag
    43381 tccttcgtct tttccgacga gacccaggcc tggaactcga gcgccttgtc cctgtcggta
    43441 taaaacgcgg gcgtgcgcag gcaggagccc tgggaatcgc cgatggcccc ggatcgcgcc
    43501 gcataacggc cggacccgta ggcgtgcgag ggatcgtcca cgatggagaa cggcacgtcg
    43561 ccggtgaccg accatcccgc cgggaggtcg ggcgagtcga accgctccca ggtgccgccc
    43621 gcgtcgggca gcttgacgct cgagacccag acggcgtcct ccccgttggt gacgctgtcg
    43681 ttcttccggt accagaattc gatgtaacac ggagtgccgt tggtacccga cggattccgg
    43741 gtgaagtaag agtgccccga cgcattgttg ccggaggagg ccaggacggg tgttcctttg
    43801 cccccgcgtc ccttggtaac cgcgtcatcg aaggcggcat cggttaccgg cagggcggtg
    43861 aatccgaggc tcatgctgat cacatccgct ccccgccacc ggtttgtgcc gtccacgtcc
    43921 aagcccgccg aataccggat ggccgaagcc atggcgctcg gagtcaccca gttggtcccg
    43981 tggatcactt tgatcggcag gatgttgcaa ccataggcga tgccggcaac gcccagcccg
    44041 ttattgcccg ccgacgccgc gacgcctgcg caacacatgc cgtgctggtc gtccgcgtcc
    44101 tttgggcccg agtcgttgtt gtcattaacg aagtcccagc cgtgcaggtc gttcgtgtac
    44161 ccgttgccgt ccccgtccac gccggggatg ccttccggtt cgtttgcaaa aaggttgtcg
    44221 atgatgtccg ggtgttccca ttccacgccg tcgtcaatga tcgcgatcac gatcgagttg
    44281 ctgcccgtcg tgatgtccca cgcctccggc gcgtccacgt cggcgtcgg
//
LOCUS       DAOOTK010000016        43111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_672_length_43111_cov_5.798750, whole genome shotgun sequence.
ACCESSION   DAOOTK010000016 DAOOTK010000000
VERSION     DAOOTK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 43111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43111
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_43111_cov_5.798750"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..828
                     /locus_tag="PK634_03440"
     CDS             151..828
                     /locus_tag="PK634_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOW96820.1"
                     /translation="MDSWLSAIMMGCFLLAAVSAAGAPSERIRVACVGDSITYGAGLA
                     DPARESYPAILQSLLGEGYEVRNFGVNGATALRNGSLPYWILPEFDAAKRFAPRVVVL
                     MLGTNDSHMRNRGEREQYEADLRALARQLADLDTRPAMVLNLPPPCYGVMSSYAQTVL
                     RREILPAIRRVADAERWRVADVHAALSGRPEWFPDGVHPNASGAAAIARTVYETVRAL
                     STEGVSP"
     gene            825..1823
                     /locus_tag="PK634_03445"
     CDS             825..1823
                     /locus_tag="PK634_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOW96821.1"
                     /translation="MSIARLSLVAAALLLASRALAQEPPATNYAPRIACAESSWSFGD
                     QVNTQTIEHVFTLKNEGHATLLIGNVRPTCGCTVAQLSTSSLEPGAEATLAVKLDLRG
                     RSGAQYKQIRVESNDPTQPTYTLTMTGAAIAEVQVNPPNLFLGRLRAEGSATATVEIV
                     TAPSTPLRVTGIASDNAVIGATAETVEEGHRYRLVVRTQPPLPVGHFLGRITVATDHP
                     GAYSNLVVTVTGVVGGGLTVAPTEIVIGSTDAQPLTRHIVLHSPDNVPFSLLGVEVPA
                     GGITATVFPMGGSGYRVQLDNLSSKPELNGAEIRIRTSVEEMPMATIPIRITTPSP"
     gene            1820..2326
                     /locus_tag="PK634_03450"
     CDS             1820..2326
                     /locus_tag="PK634_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOW96822.1"
                     /translation="MNEEGSSLRPNSATALRVAGLLLMAAVAGGLANLAHPRKIAWVE
                     DWSRYIEARALAAGFRLADTATTREIVENRSHLIFDARPAMDFKAGHLPGAVSLPYDA
                     VQNEFQNVAVLLLGGQPILTYCSGLVCDESFLLTEFLRQQGTTNIVLYSGGYEEWTRA
                     GLPVEGRP"
     gene            2323..2748
                     /locus_tag="PK634_03455"
     CDS             2323..2748
                     /locus_tag="PK634_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOW96823.1"
                     /translation="MNRAWMRPAARWALAAVFLAAAVPKILTPHDFAVAVFRYQMLPH
                     SLVNLAALFLPWLELLSALALVAVPSWRDAALVVLGGLLLVFTAAIAFNLYRGIDIAC
                     GCFTVKPGARHMGWWNLGRNFLLILAALIARDRRPGQAR"
     gene            complement(2753..3685)
                     /locus_tag="PK634_03460"
     CDS             complement(2753..3685)
                     /locus_tag="PK634_03460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOW96824.1"
                     /translation="MRRHAWLIPAIGLLLSACRRRESVPPAPSPILPFDGAAAFDEVR
                     RFVAIRPRDAATPGAERAALYLRDRLRELGVEAEVDAFADLCPVGTATFRNVIGRIPG
                     QGKGLVILGSHYDTQAGIGAEFEGANDGGSSTGVLLELARCLAAGPARSWETWLVFFD
                     GEEAMRRYGPADGLHGSRHMARQLAESGRAADVKAVIVLDMIGDRDLTVTVPRNGASW
                     LISEVFESARLEGARDKFSLYPYEIGDDHDPFHQAGLPAVDLIDFHFGSGPGRNDYWH
                     TMEDRMDKIAPESLDLVGRVALRLLARMADRVEP"
     gene            3744..3819
                     /locus_tag="PK634_03465"
     tRNA            3744..3819
                     /locus_tag="PK634_03465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3777..3779,aa:Met,seq:cat)
     gene            3969..5264
                     /locus_tag="PK634_03470"
     CDS             3969..5264
                     /locus_tag="PK634_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOW96825.1"
                     /translation="MSSDKIHTQALHAGQQPDPTTGSRAVPIYQTTSYVFKSTEHAAN
                     LFALKEFGNIYTRIMNPTTDVFEKRIAAIEGGTGALAVASGQSAITLALLAITRVGDE
                     IVAANNLYGGTYQLFHYTFPKLGRTVRFVNSQDLDAFRKAVSPKTRAVYVETIGNPKL
                     DVPDFAAVSKIAHDAGVPLVVDNTVGVGLVRPFDHGADIIVSSATKYIGGHGTSIGGV
                     IVDSGRFKWNNGQFPEFTEPDPSYHGLVYWDALGNVPGMGNVAFILKVRVTLLRDLGP
                     ALSPFNAHQFLLGLETLPLRQARHSENALEVARWLKKHPLVSWVSYPGLEDSPYFATA
                     SKYLKKGFGGLVGFGIKGGLEAGRKFINGVQLFSHLANIGDSKSLVIHPASTTHQQLT
                     AAEQAETGVTPDYIRLSIGLEDIADIKADLDQALKKATG"
     gene            5339..6139
                     /locus_tag="PK634_03475"
     CDS             5339..6139
                     /locus_tag="PK634_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOW96826.1"
                     /translation="MASYGNILQAIGRTPLIRLNRVAAGVASPIYIKAEFLNPGGSIK
                     DRIAVAMIEAAEREGLLPPGGTIVEATAGNTGAGLALAAAVKGYRLIVVMPDTGRNYM
                     TKIYHDGWMQENGFWEDRARSSVTVGRVLGAKLNLRALISVSPRDKLRHAVNLMQAYN
                     ISQIPVIEDARVVGSLSEGTVMKLLHDGLDLANQEISAVMARPLPSLDEKAEISEAYR
                     ILLGGAPGIVATRDGRPTGVVTRADLISSWTAPRGAEADQPRSSSYAI"
     gene            6129..7277
                     /locus_tag="PK634_03480"
     CDS             6129..7277
                     /locus_tag="PK634_03480"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOW96827.1"
                     /translation="MRFETRAIHDGQPPDPVTGAVMTPVYQTSTFQQDGIGRPRAFEY
                     SRSGNPTRAALEAALASLESGRHGLAFASGVAATTAVLNLLGPGDHVVAGEDLYGGTY
                     RLLEKYFGRWGVKTTYANAADPAQVAAAFTERTRLLWLETPTNPLLRLADLQALAEIA
                     RARKAWVAVDNTFASPYFQRPLELGADIVVHSTTKYLGGHSDVIGGAVIVSDDALHQD
                     LKTYQNATGAVPGPWDCWLVLRGLKTLALRMRAHEQNALRLARFLQGHPAVERVYYPG
                     LPAHPQHELAKQQMSGFGGMISLDLKGGFPAVEKFTAGLQLFILAESLGGVESLVCYP
                     PKMTHSSIPPAERRRRGIGDGLVRLSVGLEHADDLEADLRQALGTGSF"
     gene            7337..8407
                     /gene="mnmA"
                     /locus_tag="PK634_03485"
     CDS             7337..8407
                     /gene="mnmA"
                     /locus_tag="PK634_03485"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOW96828.1"
                     /translation="MSAPSRLRVAVGMSGGLDSSVTAALLAEQGHEVIGLTAHLWREG
                     SRCCSLDDARRARQVAEFLDIPHYLVNALGFFGERIVAPFVQEYAEGRTPSPCVRCNE
                     VVKFGLLLREALALGCTHLATGHYARLARREDGWHLSRGRDRRKDQSYFLHRLDQSQL
                     AHVLFPLGDLTKPEVADLARAKNLPVIHDSESQDLCFVNADGYAEFIEKQRPNLHQPG
                     PIVDTAGRVLGRHEGYYHFTIGQREGLGVAAAQRLYVKELHADTNTVVVAPLGETLAP
                     GCRLADVHWIREAPRGPIECAVQLRYRHAGARATVRPTGDGGAQVDFTEPQFAVTPGQ
                     AAVFYDGDDVLGGGWIVAERTP"
     gene            8404..9252
                     /gene="larE"
                     /locus_tag="PK634_03490"
     CDS             8404..9252
                     /gene="larE"
                     /locus_tag="PK634_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOW96829.1"
                     /translation="MTSWTNMAWPAELAGKAAELERALRDMGSVVVAYSGGTDSAFLA
                     AAAHGVLGEQAEAVTADSPSLPRKELEAARELAARIGIRHRVITTREMDNPAYLKNAP
                     DRCFHCKTELFTLLGHLAKENERRAVVDGVNTDDLGDYRPGRAAAGNLKVRSPLLETG
                     WSKADIRQASRLLGLPTADKPASACLASRVPYGTAITREILSTVERAEGALHELGFNV
                     VRVRAHGKFARIELAPGDIERAAREPLRSEIARRVKAAGFRFVALDLQGYRTGSLNED
                     LPSRPT"
     gene            9249..10001
                     /locus_tag="PK634_03495"
     CDS             9249..10001
                     /locus_tag="PK634_03495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOW96830.1"
                     /translation="MNLILIESPELRGDRVHLSDARAEHITAVLKARLGDEIRVGLLN
                     GNRGRAAVIAIEKGAVELDCRFDEPAPPPPRVDLLLALPRPKVMRRLWAQLAAMGVGR
                     IILTNAWKVEKMYFDSHVLRPEGYRPPLIEGLQQAGDTRLPDVHVFKQFKILIEDELD
                     GLFPDGLRLLARPGEGSSVLDAVPAARSGRVLLAVGPEGGWIPYEEELLTAHGFLPVS
                     MGTRPLRTDTACLALLALAHAKQRREAGYHSL"
     gene            complement(10026..11975)
                     /locus_tag="PK634_03500"
     CDS             complement(10026..11975)
                     /locus_tag="PK634_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOW96831.1"
                     /translation="MNTSFPTIGKTAVALLLFSGGAPGAEMMPFAFSDLSVTVAAEGR
                     DLYRLTVRPRGEDPAPEQEAPAGVRVADGWLEFRRPDAPGAAPLWRGRLSSLEAYGRA
                     HVPGSRLEWVARPGEALYGLGQRFGGLDVAGKVVDMWIRDVPGQGEASYYCAPVLFSS
                     SGYALFAADNPEGEFSLNPLGEGWNRYSRAGREWTFYLAFAPTLKELVLKRASIQGPF
                     RGVPDWAWGPWISRNSYENQGEAEEAIRGMTDRGLPVAAIVLEAWKGRYETGEFNRFA
                     HGGWNVDEYFAFCREHGIRNVLWQVPVIPPSHPGLKELAGSGYFVRAPDGSVRWRREW
                     LEGMANVDFTRPEAVVWWKDQMRDAVRLGARGFKADDGEDIKADDVFADGRRGWQLHN
                     EYTTLYAQALYSLFDEEGVDGMLWCRSASLGCERAPGLWAGDQYANWEQYRSLLPAGL
                     SAGLSGAPFWSHDIGGYIGDPSPELYIRWLQFGAFSPFMQYHGIKPREPWLFGHEAEA
                     AYRLLAHLRMNLKPMLIELGREAAATGLPIMRPMILEFPGDSRFLTEDSQYMLGPDLL
                     VAPIFQEGATKRVVKFPAGRWRRLLSPTIHEGPADVEIACGLMDAPVFVREGARLKVQ
                     LARGAALGTWSPDAPVRELRYGRGR"
     gene            complement(12145..12411)
                     /locus_tag="PK634_03505"
     CDS             complement(12145..12411)
                     /locus_tag="PK634_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOW96832.1"
                     /translation="MKEQYFVYVLRSLVAPEKTYVGFSTRPDERLVEHNTFTQTYSSR
                     SAPWERIACIAFARRSVALAFERYLKTPSGKAFLKKHVLEGFES"
     gene            complement(12484..12576)
                     /locus_tag="PK634_03510"
     tRNA            complement(12484..12576)
                     /locus_tag="PK634_03510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12540..12542),aa:Ser,seq:cga)
     gene            12691..13050
                     /locus_tag="PK634_03515"
     CDS             12691..13050
                     /locus_tag="PK634_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96833.1"
                     /translation="MFFQRAVMAVTLALVVAGAARGQDIRLANGMPVPGTLVQAKPEG
                     LEISTPTGPRVLTWETLSAGTRFRLQESYRITFSNILEGASVAERAALYEARKAAPAP
                     EPAPEPKKKTRKKKAAE"
     gene            complement(13176..14039)
                     /locus_tag="PK634_03520"
     CDS             complement(13176..14039)
                     /locus_tag="PK634_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96834.1"
                     /translation="MESVVQTLLLFVDGLGLGPESEHNPLFSGACPRLARLLREEAAP
                     IDAGLGVPGLPQSATGQTALLTGVNAPAVMGRHIEGLPGPRLKEIIREHNLLSALAAR
                     GYRATFANAYYTRDIEEVRSRRFQSVTTVATLAAFGAVRDTADLLAGRAVYQDLTRAS
                     LRERGYDGPFVTPAQAGRDLAALATEHDFTLFEYFQTDRAGHKGDAERTRRVLGEFDE
                     FLGGVLAFAEGERRLLLLTSDHGNIEDAATRTHTGNPVPFVALGAGAGALKIRVRALT
                     DIAPALLDLYP"
     gene            complement(14107..17145)
                     /locus_tag="PK634_03525"
     CDS             complement(14107..17145)
                     /locus_tag="PK634_03525"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOW96835.1"
                     /translation="MTSSEWNQQQGGAAPGAPAGAAPEQNRPDFLDQQEGRFFRRADW
                     SAFWTVLLVTLGVYVYTLAPTVTLEDSGELAVASDYLGVPHPPGYPIWALLTWFFQWI
                     FHWVTYYGFPNPAWSVGLFSAASGALACALLAMLVSRSGSDIIRTWKRATEVLGYRTE
                     TVLCWAAGVSSGLLLAFSPVMWSQSVIVEVYSLNALFQTLLLLLMYRWMCRPKEDHTL
                     YITAFIFGLGLTNHQTLLFMVLGLMAALWFRDRALFRDCLAGLFLCISAYLFFAAYGM
                     NPGPNAPAEVLKQKHLTLTFALLFLFAPVGLFLIQRQLMTEWKRVLILVALVGAGLSF
                     YAYMPFASEQNPPMNWGYPRTWEGFKHAISRGQYERITPTDIFSRKFLEQILSYITDL
                     REQFSSPISLLAVLPFVFFIKLDRRARTWLITALISFFSVSIILVIFQNPALDIQTLF
                     IGRVQFIQSHAVYAIWLGYGLIFVLAWLETLLGGMGIARFLGPVFAVLLPLVLVYQNG
                     YDDNQLLILGGAEQNGHDFGWQFGHWQLRGVNGIRDDLHFKRSDAEFEEIWKSYPTPD
                     YPPEMGTNAIFFGGTDPGRFVPTYMIYSAKCRSDVYLITQNALADNTYMNVMRDLYGD
                     TIWIPSQQDSNLAFQRYVDDVQAGRVPAGAAVTVENGRVSVQGVAGVMMINGILAQMI
                     FEQNKYKHEFYVEESYVIPWMYPYLTPHGLIMKINAEPMKELPPDLVQKDHDFWTWYC
                     DYLLKNPKFDRDVVARKTFSKLRSAIAGLYAFRGAQDTPRREEMYREAEYAFRQAIDL
                     YDLSPEANYRLADILMQQRRFAEARELIENFLKKDKGNDKLQDFLNQISSTDKDDARR
                     NELEASFNQGRGDLQSALELAQLYRRLNLDQQFVGLTMQLLNDGSVPPAFYLEIARIF
                     AEARRADLLAVALQRYVARDPSQPAVWMDLAAVQLALQQTDPCLESIRQAITVGGDAA
                     RDAIRKDQRFNGLRANAQFQQLVPPVQTRLPAALPNLPVF"
     gene            complement(17267..18028)
                     /locus_tag="PK634_03530"
     CDS             complement(17267..18028)
                     /locus_tag="PK634_03530"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOW96836.1"
                     /translation="MEKRTLKPYFVLIALFVAMSVALALTVDVNITDEAGISVALPDQ
                     VGAWRGEEIRYCQGPTCEKEFVRSALENPDVCPLCGSPLAPMSLGEKTLLPPDTIVLK
                     KKYTHPMGQAVFVSIVLSGKERASIHRPEVCLRGQGNKQLGSRVLEVPIANRAALRVM
                     VIDMLRQYMTADQRVVEYPSYYAYWFVGKGRETPHHWQRMMWMATDRILHSVSHRWAY
                     IAVTGIRTPDSDEYREQIRNFVSQLYPLMAIEPHG"
     gene            complement(17992..18972)
                     /locus_tag="PK634_03535"
     CDS             complement(17992..18972)
                     /locus_tag="PK634_03535"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOW96837.1"
                     /translation="MSYTRTSSQLWSERWRLAALPPDALRRAGLASILVGLLYLLFHL
                     QGNTTDIRMFGRSAILWMVSYWHDDLSYSWLIPFISAGVIWCQRKQIAAAPQRTNRWG
                     LALVVLALLVHWMGAKAQQVRVSLFALVLLLWGAPLYLYGWQVMKHLVFPVAFLFFCI
                     PLNFLDELTAPLSLLATTASTHLLNGLGIAVQQVGTRMTVNDGPDMVRWTLEVAAPCS
                     GLRSIMALTILSAVYAYFTQKTLVRKWILFLASVPLAIVGNVMRIITISLMAQAFGQD
                     VAVGLYHDYSGFIFFPVSLGLLIGLGGLLNHHPREIWLRWKSELLSPISS"
     gene            complement(19041..20450)
                     /locus_tag="PK634_03540"
     CDS             complement(19041..20450)
                     /locus_tag="PK634_03540"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOW96838.1"
                     /translation="MLYRTSQFRSQLAQLLDALVTAAAFTFALASRRWLTMWRPDTFP
                     PFDALWQHAWLYLLLLPLWIIILESNGYYQHQFAHSRLDSLKILARANVIGVVTLFFL
                     LYLLKVKHIPRVLVIIFGVMDVILLWIKDAIMSKLVPLWSSPASILLVGGQADRESVQ
                     RYLQQQPAWQVRLLGVLTPAGEPAGDGAGLPILGAVQDLSRILHEHSVDSVLLAPGRQ
                     NFEEIQQLIQTCETEGVEAWLLADFFRTSIATAHMDQFQNRPVLVFRSTPALSWALVA
                     KRIMDMAGALVLVALLSPLLLAIALAVKLTSPGPVFFRQKRCTLHGRVFTMIKFRTMV
                     ADAEQQRAGLEAQNEVSGPVFKIRADPRVTPVGRFLRRHSLDELPQLFNVLAGDMSLV
                     GPRPPIPAEVAKYENWQRRRLSMRAGITCLWQVSGRNALSFEDWMRLDLEYIDHWSLG
                     LDLAILVKTPLAVIRGTGF"
     gene            20550..21674
                     /locus_tag="PK634_03545"
     CDS             20550..21674
                     /locus_tag="PK634_03545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96839.1"
                     /translation="MRIAMMGLRAIGQGSGGVEKAVEEIARRLAARGHAVTVFCRARY
                     NSACAKEFEGVRLVNLPAVYTKHLEAITHTFLAACRALAGYDLVHIHATGPSMLAFLP
                     RLAGRRTVVTVHGLDWKREKWGAGARLFLRLGAWTAGAFPHRTIVVSRELQRFYRERY
                     GRETVPIPNGVTPGVRRPLDRLRRFGVRPDEYLLYLGRLVPEKGGHLLIEAFRAVPGD
                     LKLLIVGGASHSEDYLARLRALAEGDPRIVFAGPLYGEEKEEAYSNARALVFPSTLEG
                     MPLVLLEAMALGCPVLCSDIPENLEVVRPDGAPGSALAALFPAGSADALRAALPPFLS
                     APEAARARAAQAAAYVARTYDWDRITDATEEVYRSLAARV"
     gene            21778..24120
                     /locus_tag="PK634_03550"
     CDS             21778..24120
                     /locus_tag="PK634_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOW96840.1"
                     /translation="MKRFGRIRRGLALLLALLPAVARSGGGPQNVLIVVNDGDPDSQE
                     LGRYYREARGIPERNVCHLNLSAPLHNTFLDIFEKSIRDPILAHIRDEKLSDQIDFLA
                     LCMDTPTRINDSESITAALFYGFKNAPPAPPCQLSPSTLSFYFNAERAFTHDLAGREN
                     PHYLAMMLTGPAPRDARALVDRGAACDGTAPTGTFYLVKPPGDPDRNIRYRLFDELDF
                     HARFMPGFPRRVFSEENTLTGLTDILGYLTGGPNHPETFWTANQYLPGAIADQLTSFS
                     GLLPVPPMGQGSMLQWIGAGATASYGTVSEPCNFLEKFPSPMVLFWYARGFNLAESYW
                     MGVAHPYQGLFVGDPLAAPYARPPRVTVAAPAAGQTVSGTVTVRVSAAGTPECPCAAL
                     DFYLDDLRVGTVTNIRPQAWNEVYLEFDGQEYSYILGESDDLPAAVRGLAREARKNPM
                     LNAVPFGDRLMLMDGRIGKRGSTTPYGARVEQGLGPPPGFGARNLTPALLDGTYAARK
                     YVMLQGAAAAGDAVECRVTLGNGLIATARVEAAEGEAALDVAKRLKAALDSHPVLAGP
                     DGIRIADLKAKADRADFALESRTAGPPGVAILLRYAILPKTPGAGLAPPVAYEGRLSD
                     NMDLMSSRGMLLFHCGRETLAAEFPWDTSGIPDGPHVLRLVARDGTAVQTQGHAFVPV
                     VVRNTDRRCRLTGVESGQVVRAGARLSLGVECEPADFPVGAVDFLVEGKIADRAGKAP
                     FHGTVDTAPYRGGRVSVQVRVADADGRQTLSDPVWISVPR"
     gene            24189..27050
                     /locus_tag="PK634_03555"
     CDS             24189..27050
                     /locus_tag="PK634_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOW96841.1"
                     /translation="MRIRVSALLAAAAALLSPLPGRAGGGPQNVLVILNDQSIESQET
                     GLYYRQLRGIPERNVLHVSVPPASAAAGFHDMTNTAFQTSIVDRISQHIGLYGLSNQI
                     DTLVLCQDLPTRVSDNEGATAVLFYGFKNAPKIVASCSMPTNTASGYYKAERGFRHAD
                     KYAGTNYYLSFMLIGDRLSIAKSNVDVSVRSDGTRPGGCFYLVESSDAERNVRYPLNN
                     NFDFHARFLSGFPAHEITGYGPLNQETNVLGFQWGSSQINDYWGATNRYLPGCMADHM
                     TSFGARFPTDPYNQGKIWNWIRQGASASYGAVNEPCAYIEKFPDPLVFFWYARGFSLG
                     ESYWMSVANPYQGLFIGDPLAAPYADPPAVAVASLASNQVVSGTVAVDIQARTNAAGR
                     PAARVDVFVDDLYAGTPTNVPPRPGNVLQVTISGQPVAYQVAAGDTLYSAVTSLAGRV
                     NASNLAVRAAARGDRLELVYTNYGYFGTGVTYSAGTETGAAAELTVFAQALGSNLLES
                     AYAAREFVQLQAYNGKANTGDTVTCVITLTNGVAATNRIIAAQNETPKQVVTRLVAAI
                     NSHAVLQGTNGAWANTHTYQEVSSFPLEARRPGPEGIRLHVAYTIVPAIPGSGLYPAD
                     SFSDFFNDNESDLTARGNVLFWCGRSPLDTSWAWDTTAWPNGPHTIRAAGRLGTGPEA
                     QGHSVTPVLVSNSAFAARLLSPTNGEPFVLGSNVFLQAEVTNAPGVVTQVVFYVEDKP
                     CSVLTNAPWVSAFDTADAGVGAVSVKALAWDSGGTRAVSPASWIAIQRSPTLDTDSDA
                     LPDSWEQQYFGGLYVYAATNDPDSDGMPNHFEQAHALNPTNNDAAADGDGDKAGNWQE
                     YVAATDPGDSNSVFRVASGSDTNRRPLLSFFADTGRLYEVRYNSDYLTNFNGWLVASP
                     APFAGSGGLTNWLDDGSETEPPPEDVTQRLYRVRVALPD"
     gene            27053..28054
                     /locus_tag="PK634_03560"
     CDS             27053..28054
                     /locus_tag="PK634_03560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOW96842.1"
                     /translation="MKAEPPSTSAARLLQADHAKGIAIALVVICHVEQGLGRAGIAGV
                     PAFWPAFEALCYTFMVPVFFVLSGHFLEASIRRRGLKGVAASLPGTLLYPYVVWTLLQ
                     AALMILTGAGNRHVAWVEVPGLLLRGWMQFWFLHALMLIYALDLLLRGMRVGRFGRLA
                     VALLLDGAAAMRPDWFGAPVRLACQHYLFLALGALAPERCFEALPRRQAIALAVAGFA
                     GLYFLHAHRAACSALLLPAATLAGVAGTLGLSSLLAAAPSLNGWSSLGRYSLEVYCAH
                     VIFSAGTRVVLQRGLHVSLFWPHEVAGVAAGLLLPLGLARVARARGWPLFRPPRRAP"
     gene            complement(28078..29601)
                     /locus_tag="PK634_03565"
     CDS             complement(28078..29601)
                     /locus_tag="PK634_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96843.1"
                     /translation="MASDKIVRVPIALLGIAGFVIGLVASYWVAGFFGFSPALMWSVL
                     VILFVVGVFFLEHPKLYLYFVFFYHAVSFNGLFFGCLYVPIPFIGILDEALLAVPMAI
                     IVMKAIHRQLPRGATFFPLLYLGLALLSWKVNHVPTLNFIRVTLSYGKFYIFWFYARA
                     IGPWSPREKKLWFIMLLALAFLQLPFNVVWQKGLSIRLTADDSRGSIGSAHIVGYMSV
                     IALFLLGGWLFSLKPPVPRKRVALALFMALLIAYDLIFLTDTKHVLLMVPLAAAPMFL
                     FPRIGIRNRVLFGLAMLVFLVASWVYLSFATKSWENRPAMVMHSMKYSGKGAVFRTIT
                     RDLAGEVPLLGLLGAGPGNFCSTVGTYSMRPLANKYVLPYVLTAFRSRGQAAETSVIG
                     SAMSSLYTLWGEFGPISMAFYFWFWFAAMRHLWRLCLQDPGFSFETGQRLGIIGGVIT
                     VFLVGMLIETFNLSMLMLPLWSLVGIHWVSGPEAPTQRKEDELALQVGSSFRMPFRF"
     gene            29741..32080
                     /locus_tag="PK634_03570"
     CDS             29741..32080
                     /locus_tag="PK634_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOW96844.1"
                     /translation="MVLFGQRPGRLGAALLLGALALPAGGQEAARIAYYPFDGDATDG
                     SGNSRDGEIRGAAAAADRFAFRDQALRFDGQNDYVAVPDTGLAVGGDFSIAFWARSIG
                     RQKMYVFGLGAPGGANLDLEVNDESSLWAYWNGGGSTGIRIESRVTNLVEDFSGFAVT
                     NSTLDLGSNLNAYLSTTGWTGSKVFSDGGRAKLGSSAAHGSLTTPSLDLDADLGRSRL
                     LFDLVRWTSDTAVIQVLMSTNGAPFQLAGGEITAPDIMTRQTLDLIDGAADTRICIQA
                     KWTNQCRFKLDNVHISKRADTYEFTDGLWHHVAVCRSGAEVRVYSDGILRETAAQAGA
                     IGASGPLLLGCSSSTSAFWYGEIDDVGLWNRALSEAEVLALSDDRLPEAPWRWTQVQH
                     AAAFSSRDGAGDLVFDGRLWLLGGWAPETEPDPYTLNEVWVSSNGADWAWVTNAPWEP
                     RHTAGYAVHDGKMWVLGGDANRGHYQNDVWSSTNGADWVQVAATVPWSNRVLHYATEF
                     NGCLWVMGGQELPQLVPGTPTNAVFYNDVWRSTNGADWVRVLDHAPWSPRGMISGRAV
                     LSNQLWLVGGGTYQTPNAPDRQYFNDVWSTADGSNWTCATPGSPWQARLYHRVGVYAN
                     RLWITGGAYDQNLNDVWFSADGASWLQLAGTPWPPRHAHSLYLTEAALWITAGSSNDL
                     FCMHDVWRLDSTNAAAWPEPPPLAIDGPATNRMWLGFEGEQHRTYSIELNTNLLSRWE
                     AWQLSSAETTQRVVELDLDAFGPRNFFRLVWPYYVLRPE"
     gene            32141..33319
                     /locus_tag="PK634_03575"
     CDS             32141..33319
                     /locus_tag="PK634_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOW96845.1"
                     /translation="MRLAAIYTLYGRRAGAELFVERTLEELGRQVPDWSITVFCNAQA
                     RDALRERLPGVKAEYVPWLDRQWKKAVWLEWRAPRVVTRQAFDVFWVPSGSASFPGPW
                     EVPTVGTFLDMGGFLVKNRFRRSRTLYCRYWSTPRTLRRAAALTSISQATADDLVRLF
                     PGARTPRVIHLGPSPRATSARLADPRTVIREETGLSLKHILFSPARTDYRGKGRDILL
                     RAYAEYRRRAPAPLPLVLPGPPGEFHDSVLKDIHDLGLEGSAVWPGRVSDACMEALYA
                     ISRALILPSRTEGFGFPVLEAMERGVPVICSDAGSLPEVAGDAALVVPAGNADALLEA
                     LLRLEREPGLREELARRGRERCRAFSWEQTGRAYRDVFETVVSGAAGHPPDNTAGPPA
                     "
     gene            complement(33267..35798)
                     /locus_tag="PK634_03580"
     CDS             complement(33267..35798)
                     /locus_tag="PK634_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOW96846.1"
                     /translation="MGVLAARAEVPFSLIILPDTQYYTDSTTNRSAMFSAQTDWILAN
                     RTNLNIRFVLHEGDITEHTYSYRGTNTLEWQRASQCMAPLDGKVPYLVTYGNHDFFYN
                     AGIFSRNTDRFNEYFPLSRFQATASVVDSFPAGRSDNIGAVFTAGGVDWLVVSLEWGP
                     GDATLAWADQLLNRYPRHRAIVLTHSYLYLDGLWQGRLASHNSDPHEGYGPVNNGLEM
                     WDKFVRKHSNIAFVFNGHAATSPWETGARRVDQGDFSNTVHAVVANYQMMTNGGAGYL
                     RILQFTPDTGRLNVTTYSPHLGNYKTDTNNQFACTNRALFDASPPGSPSGLRVEAVDG
                     STCRLTWEPAADGESGLLGYAVFRDTQQVAFVQGAECTDWSAPPDTLCSYRVVAINGA
                     GCWSEGAASAVVVTPREVRAPAVDNVWAAASNEVRVSFDKAVDSRDAMAPTNYSFGEA
                     LALEGVTLAPGAREVILTTEPMEDGSCYPLLIKGWESHHPDGDRSAGEWLFDHSTPVL
                     QEGFADGDLAGWAAMDEGTLNAPSLWSVQSNRVKQASFIGGPAWNSLFNRQGAFLLWT
                     AEVAAAWSDYAMEADITTGYGGGIGLVFRYQNPSNYYRFEMDQTNRCRRLYRQRNGTN
                     EMLAWNWHGYTVNSNQHIRIEVLADTVRVFVNGADVFGRTIVDALPLASGTVGLWTWR
                     SRPTWFDNITVRPIQIAASTAEYNRYILEYWDEAGNGWQGRGGGKPRPSLNPHADADG
                     DGMTNYKEYVSMTHPMNRDSVFRVSLVATNPGRVSISFPSSTNRLYRVYARRTSTGGT
                     WWPLQSAAFRGDPVQTMREDLLRSSPPPLMPADRRYYRVDVQRPR"
     gene            complement(36076..37215)
                     /locus_tag="PK634_03585"
     CDS             complement(36076..37215)
                     /locus_tag="PK634_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HOW96847.1"
                     /translation="MSAQTEYLGAGSVACLGEVLQRHAPRRVFLVTGRASYEASGARA
                     AIEPLLGATPFVRFHDFESNPRLEDVERGIRLFRESACDLVVAAGGGSVMDMAKSVRF
                     LAAQEAGARDCLRDAGGPRRRGAPLAAIPTTAGSGSEATRFAVLYVDREKESLEHEFV
                     LPDYAIVDPGLTARLSPRQTAISGLDALCQATESFWSVLSTPESQGYARRALGLVLEH
                     LDRAVNRPDAAARAGMAEAAHVAGKAICISKTTACHAVSYPLTSYFGVPHGLAAALTL
                     PSMLAFNAGVTVADCQDARGAAWVGRILGELMALIGADSPAGAAAALTDLMRNVGMPV
                     RLGEAGIRPADIPLILDHGFNPGRVKNNPRRLTREELKALLDAMA"
     gene            complement(37220..38347)
                     /gene="aepY"
                     /locus_tag="PK634_03590"
     CDS             complement(37220..38347)
                     /gene="aepY"
                     /locus_tag="PK634_03590"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HOW96848.1"
                     /translation="MTCEELWAAFRAEGLDFFTGVPDSTFAPWMAFLSANNGQGLTDL
                     AASNEGEAVALAAGYHLATGKVGVVYMQNAGLGNTVNPLTSLADPEVYAIPMILMIGW
                     RGQPGRKDEPQHVKMGRVMRPLLDALEIPWRDLPAAADPAAAVIAELRALALRASAPV
                     ALIVRSGIVEGRAVAPASDRSLTREQALEWLLELLPAAAVSVATTGKTSREVFEIRER
                     RGEGHERDFLTVGSMGLASAIALGVALQKPGAKVVILDGDGAMLMHTGNLAAIGRAAP
                     ANLYHVVFDNGVHDSTGGQRLASASVDFGRVAEAFGYAGGAAAETEDELKAAARSMLA
                     GRGPFLLTVRVRPGARKDLGRPTTTPVRNKTELMKFLGALK"
     gene            complement(38344..39645)
                     /gene="aepX"
                     /locus_tag="PK634_03595"
     CDS             complement(38344..39645)
                     /gene="aepX"
                     /locus_tag="PK634_03595"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006670254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HOW96849.1"
                     /translation="MKQVYVGMCADLIHHGHINIIREARKLGEVTVGLLADKAIASYK
                     RVPLLSYEQRKQIIENIQGVARVVPQETLDYVPNLRALKPDIVVHGTDWREGVQRETR
                     ARVMAALKEWGGELVEPEYTPDVSSTALIREVLQVGSAPDVRRRKLRRLLDLKPLVRV
                     LEVHNGLTARLVEQVSVGEGTAARSFDAMWESSLTDSASKGKPDTGVVDFTSRLQTIE
                     QILDVTTKPMLVDADNGGFAEHFVYLVRTLERQGVSAVVIEDKTGPKRNSLLGTDVAQ
                     EQESAEMFAGKITAGKRAQATDDFMIIARIESLILKKGMEDALRRARAYLEAGADGLL
                     IHSKEKTPDEILRFCEGYRAMGAPAPLAVVPTTYADTPEDRLAEAGVRIVIYANHLLR
                     AAYPAMRKAAESILRHHRCREADEVCMPIKQVLTLIPGAEG"
     gene            complement(39666..40808)
                     /locus_tag="PK634_03600"
     CDS             complement(39666..40808)
                     /locus_tag="PK634_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOW96850.1"
                     /translation="MTAPRQIVLILLDGLRADALDAHPVFDGLKRRGLFCGNMVTYAP
                     YTTASMHAILTGVYGPRNGVDSYFSAAKFRADACRTLAQYLRAAGYETAVDINNRNTI
                     PGQGYERVTEYDEFHPDHRGRLFERHRALLAEARAPGRPYLLSLHNLKTHTELIQKFR
                     EVYPGEKESLYYADPARNEREYRGYAREMGDYVAALLDDLDRARFFRDGLLVVFSDHG
                     CSYGERPGERMYGTYLNDYTLRTFAFFLGAGFAPGARRAGLLRTVDLLPTVLDACGLE
                     PAGDALEPDGRSFFREEAGDRWAFVETAPLAGAHPSPYAPNYHGVVSESRKVLYHSET
                     DALEYLAREGGAWRAAPADAPGAEEWLMRLCACSPRVNRKWLNKAS"
     gene            complement(40805..42325)
                     /locus_tag="PK634_03605"
     CDS             complement(40805..42325)
                     /locus_tag="PK634_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOW96851.1"
                     /translation="MAELERRNLLILLPDALRPDQLGCYGSTRGNSPVVDRLAAEGIV
                     FENAFTVSPVTNVSVSTLFTGCLPKVHGVRLHTSVLARGVPTLAERLRAEGYQTGAVI
                     SCATMDRSRGLDRGFDFYDDAFGPEEPKGIKGADPDERTISRHAGTAVSLALQWLRGL
                     DPGRPFFLFLHLFDTHSPYDPPRDWRESHPTAYAGRADGSEKEGLAIKLGEFVPGPED
                     IAHLNYLYGGELNHTDRQVARLLSALSDLGRAENLLTVFTADHGVHLGERGVWGSGRR
                     LYDRELRVPLVLHGLPGLAGRRVSTLVPNTDVMPTLLEALGCAAPAGLNGRSRLAEAR
                     NPDAADDTVIYAETFMPAAPENQRLAVRNRRWKLITPVPPEERAVLLSRPPGETWQRI
                     RRLLYRLWHNRKGRKEQFRRALDVLFGGREKDAYRRIEDRDRQLLGSEPELYDVRNDP
                     GEEKNVYAARPRVARLMANELAFFSSRYIRRSSAAEQLSPEQLREANEVLRQLGYK"
     gene            complement(42440..>43111)
                     /locus_tag="PK634_03610"
     CDS             complement(42440..>43111)
                     /locus_tag="PK634_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HOW96852.1"
                     /translation="DFEVCLEFGGYGPCGYGSHAHNDTLSVVINFRGSRLFVDPGTYL
                     YTPDPEARNRFRSAAAHNALTRGGREPWPLPTDMPGLFRLPGPDRPGRVTEQDRSGIR
                     VETTTGGTFRCSRRVQVEADRVILDDRWSKAGGEPLTWNFTLAPGVLAERDGAAGFRL
                     VAPSGVLRLACSRPEAEGRVEDGEMSPGYGRREKVQRLRVRLAGGMPEECLWTLSPEQ
                     PPRPG"
BASE COUNT         7075 a        14848 c        14520 g         6668 t
ORIGIN      
        1 ggcggcccgc cgggtaaacc cggggcaagg acacgttata ccgggcggcg cgcccgggcg
       61 caagcgggca ttgttcgcct cccgaaatgc gcttgttccg ccgggaaagg ccggtatatt
      121 ttccgcatcg ccgcaatcgg agtcggaacc atggattcct ggctgtccgc aatcatgatg
      181 ggatgctttc ttctggcggc cgtatcggcg gccggcgccc cgtccgagcg gatccgcgtg
      241 gcctgcgtgg gcgacagcat cacctacggg gccgggctcg ccgatcccgc ccgcgaaagc
      301 tatcccgcca tcctgcagag cctgctgggc gagggctacg aggtgcgcaa tttcggcgtc
      361 aacggcgcga cggccctgcg caacggctcc ctgccctact ggatcctccc cgagtttgat
      421 gccgcgaagc gtttcgcccc ccgggtcgtc gtcctcatgc tcggaaccaa cgattcgcac
      481 atgcggaacc gcggcgagcg ggaacagtac gaggccgacc tgcgggcgct ggcccggcag
      541 ttggcggacc tggacacccg gccggccatg gtcctgaacc tgccgccgcc ctgctacggg
      601 gtgatgagtt cgtacgcgca gaccgtgctc cgccgcgaga tcctgccggc gatccggcgg
      661 gtggccgatg cggaacgctg gcgggtggcg gacgtgcacg cggcgttgag cggccggccg
      721 gaatggttcc ccgacggcgt gcatcccaac gcctcgggcg cggccgccat cgcccggacc
      781 gtgtacgaaa cggtccgcgc gctttcaacg gaaggggttt cgccatgagc atcgcccgcc
      841 tgtccctcgt cgccgccgct ctcctgctcg cctcccgggc cctcgcccag gagccgcccg
      901 ccaccaatta cgcgccgcgg atcgcctgcg ccgagtccag ttggagcttc ggcgaccagg
      961 tcaacaccca gaccatcgaa cacgtgttca cactgaagaa cgagggccac gccacgctgc
     1021 tgatcggaaa cgtccggccg acgtgcggct gcacggtcgc ccagctttcc acgagttccc
     1081 tcgagcccgg cgccgaggcc acgctggccg tcaagctgga cctgcgcggc cgctccggcg
     1141 cacagtacaa gcagatccgc gtggagtcca acgaccccac ccagccgacc tacacgctca
     1201 ccatgacggg cgcggccatc gccgaggtcc aggtcaatcc ccccaacctg ttcctcgggc
     1261 gcctacgggc ggaaggcagc gccaccgcca cggtggaaat cgtcaccgcc cccagcacgc
     1321 ccctgcgcgt cacgggcatc gcgtcggaca acgccgtcat cggcgcaacc gcggagacgg
     1381 tcgaggaggg ccaccggtac cggttggtcg tccggaccca gccgccgctc cccgtcggcc
     1441 atttcctggg ccggatcacg gtcgccaccg accacccggg cgcgtattcc aacctggtcg
     1501 tcacggtcac cggagtcgtg ggcggcgggc tgaccgtggc gccgacggag atcgtcatcg
     1561 gctcgaccga cgcccaaccc ctgacacggc acatcgtgtt gcactcgccc gacaatgtcc
     1621 cgttctcgct cctcggggtc gaagtgcccg ccggcgggat cacggcgacc gtcttcccca
     1681 tgggcggcag cggataccgg gtgcaactgg acaatttaag ctccaagccg gaactgaacg
     1741 gcgcggagat ccgcatccgc accagcgtcg aagagatgcc gatggccacc atccccatcc
     1801 gcatcacgac cccctctccg tgaacgagga gggctccagc ctgcggccga actccgccac
     1861 ggcgcttcgc gtcgcggggc tcctgctgat ggcggccgtg gccggcggcc tggccaacct
     1921 cgcgcatccg cggaaaatcg cctgggtgga ggactggtcg cgctatatcg aggcgcgcgc
     1981 cctggcggcc ggcttccggc tggcggacac cgccacgacc cgcgagatcg tcgaaaaccg
     2041 gtcgcacctg atcttcgacg cgcggcccgc gatggatttc aaggccggcc acctgccggg
     2101 cgcggtctcg ctgccctacg acgcggtcca gaacgagttc cagaacgtcg cggtcctgct
     2161 tctcggcggg cagccgatcc tgacgtactg ttcaggcctg gtctgcgacg aatccttcct
     2221 gctgaccgag ttcctgcggc agcagggcac cacgaacatc gtgctgtact cgggcggcta
     2281 cgaggaatgg actcgggccg gactgcccgt ggagggccgg ccgtgaaccg cgcatggatg
     2341 aggccggcag cgcgctgggc gctcgccgcg gtgttcctcg ccgcggccgt gccgaagatc
     2401 ctgactccgc acgattttgc cgtcgcggtt ttccgctacc agatgctgcc gcattcgctc
     2461 gtcaacctgg cggcgctgtt tctgccctgg ctggaactgt tgagcgccct cgccctcgtc
     2521 gcggtcccga gctggcggga cgccgcgctg gtcgtgctcg gggggctcct gctcgtcttc
     2581 accgcggcca tcgcgttcaa cttgtaccgc ggaatcgaca tcgcgtgcgg ctgtttcacc
     2641 gtcaagcccg gggcgcggca catgggctgg tggaacctcg gccggaactt ccttctgatc
     2701 ctggcggccc tgatcgcccg ggatcggcgc ccgggccagg cacggtaggg cgtcacggtt
     2761 cgacgcgatc cgccatgcgg gccagcaacc gcagcgccac gcggcccacc agatcgaggc
     2821 tctccggcgc gatcttgtcc atgcggtctt ccatcgtgtg ccagtaatcg ttgcggcccg
     2881 ggccgctgcc gaagtggaag tcgatcaggt ccacggccgg caggccggcc tggtggaagg
     2941 gatcatggtc atcgccgatt tcgtagggat aaagcgagaa cttgtcgcgc gcgccctcca
     3001 gccgcgccga ctcgaacacc tcgctgatca gccaggaggc gccgttgcgg ggcaccgtca
     3061 cggtcaggtc gcggtcgccg atcatgtcca gcacgatcac ggccttcacg tccgcggcgc
     3121 ggccggattc tgcgagctga cgcgccatgt gccggctccc gtggaggccg tcggccggcc
     3181 cgtagcgccg catggcctcc tcgccgtcga agaagaccag ccacgtctcc caggatcgag
     3241 ccggccccgc cgccaggcaa cgggcgagtt ccagcaggac gcccgtgctc gagcccccgt
     3301 cgttcgcccc ctcgaactcc gcgccgatgc cggcctgcgt gtcgtagtgg ctgccgagga
     3361 tgacgagtcc tttcccctgt ccggggatcc ggccgatgac gttccggaac gtcgccgtgc
     3421 cgacgggaca gaggtccgcg aaggcatcca cctcggcctc gaccccgagt tcccgcaggc
     3481 ggtcgcgcag gtagagggcc gcgcgctccg cgccgggcgt ggcggcatcg cgcgggcgga
     3541 tcgcgacgaa gcggcggacc tcgtcgaacg ccgccgcgcc gtcgaacggg aggatcggag
     3601 acggggcggg cgggacggat tcgcgccggc ggcaagccga gaggagcagt ccgatcgctg
     3661 gaatcagcca tgcatggcga cgcatgccgt gggagcatat tgacagggcc gccggtagta
     3721 aagtaggtta acgtggcatg gtcggcgggg tagctcagtt ggcagagcag aggactcata
     3781 agcctaaggt caccggttcg atcccggtcc ccgccaccat cctgccgtaa cagtttctga
     3841 aacagcccgt ttggccacct gtgtcatatc tccaaaaaaa gttcgcgccc atctttacaa
     3901 aatgaaagaa tatgttattg tttataatgt ctatagatta aatagagtat aaactgaaag
     3961 gaacggtcat gagcagcgac aagattcata ctcaggcgct ccacgcgggc cagcagccgg
     4021 atcccacgac gggatcgcgg gccgtaccga tctaccagac cacttcctac gtgttcaaaa
     4081 gcaccgagca tgccgcgaat cttttcgcgc tgaaggaatt cggcaacatc tacacgcgca
     4141 tcatgaatcc gacgacggat gtcttcgaaa agcgcatcgc ggcgatcgag ggcggaacgg
     4201 gcgcgctggc cgtggcctcg ggccagtcgg ccatcaccct cgccctgctg gccatcaccc
     4261 gcgtcggcga cgagatcgtc gcggccaaca acctctacgg cggcacctac cagctcttcc
     4321 actacacgtt ccccaagctc gggcgtaccg tgagattcgt aaactcgcag gacctcgacg
     4381 ccttccgcaa ggcggtctcg ccgaagaccc gcgcggtata cgtggagacc atcggcaatc
     4441 ccaagctcga tgtgccggac ttcgccgcgg tctcgaagat cgcccacgac gcgggcgtgc
     4501 cgctcgtggt ggacaacacg gtgggcgtgg gactcgtccg cccgttcgac cacggcgcgg
     4561 acatcatcgt cagctccgcc acgaagtata tcggcgggca cggcacgtcg atcggcggag
     4621 tgatcgtgga ctcgggcagg ttcaagtgga acaacggcca gttcccggaa ttcaccgagc
     4681 ccgatcccag ctaccacggc ctggtctatt gggacgcgct cggcaacgtg cccggcatgg
     4741 gcaacgtggc gttcatcctg aaggtccggg tgaccctgct gcgcgacctc ggcccggcgc
     4801 tgagcccgtt caacgcgcac cagttcctgc tggggctcga gacgctgccc ctgcgccagg
     4861 cgcgccattc ggagaacgcc ctggaggtcg cgcgctggct gaagaagcat ccgctcgtga
     4921 gctgggtcag ctatcccggc ctcgaggaca gcccgtattt cgcgacggcg tcgaagtacc
     4981 tgaagaaggg cttcggcggg ctggtcggct tcggcatcaa gggcgggctg gaggccggca
     5041 ggaagttcat caacggcgtc cagctcttct cgcacctggc gaacatcggc gactcgaaga
     5101 gcctggtcat ccacccggcc tcgacgacgc accagcagct gacggcggcg gagcaggccg
     5161 agacgggcgt caccccggac tacatccgcc tctccatcgg cctcgaggac atcgcggaca
     5221 tcaaggccga ccttgaccaa gcgctgaaaa aagcaacggg gtgacgtcgg gccggcgtcg
     5281 gggccatgta tcacagttta taaactgtga tacatggggt tcggcaagga ctacgggcat
     5341 ggcctcctac ggcaacatcc ttcaggccat cgggcgaacg ccgctgatcc ggctgaaccg
     5401 ggttgcggcg ggtgtcgcct cgcccatcta catcaaggcg gaattcctca atcccggcgg
     5461 cagcatcaag gaccgaattg ccgtcgccat gatcgaggcg gcggagcgcg aaggcctgct
     5521 gccgccgggc ggcacgatcg tcgaggccac cgcgggcaac accggggcgg gcctcgcgct
     5581 ggccgcggcg gtcaagggat accggctcat cgtggtcatg ccggacacgg gccggaatta
     5641 catgaccaag atctaccacg acgggtggat gcaggaaaac gggttctggg aggaccgcgc
     5701 tcggtcgtcg gtcaccgtcg gtcgggtcct gggcgccaag ctgaacctcc gcgccctgat
     5761 cagcgtgtcg ccccgcgaca agctgcggca cgccgtgaac ctgatgcagg cgtacaacat
     5821 ttcgcagatc cctgtgatcg aagacgcccg cgtggtcggc agcttgagcg agggcacggt
     5881 catgaagctg ctgcacgacg ggctggacct cgccaaccag gagatctccg cggtcatggc
     5941 caggcccctg ccctcgctgg acgagaaggc cgaaatctcc gaggcctacc ggatcctgct
     6001 cggcggcgcg cccggcattg tcgcaacccg cgacgggcgc cccacgggcg tcgtgacccg
     6061 ggccgacctg atttcctcgt ggaccgcgcc ccgaggcgcc gaagccgatc aacccaggag
     6121 cagttcctat gcgatttgag acccgcgcca ttcacgacgg ccagccgccc gaccccgtca
     6181 cgggcgcggt gatgaccccc gtctaccaga cctccacctt tcagcaggac ggcatcggcc
     6241 ggccccgcgc gttcgagtac tcgcgctcgg gcaatcccac gcgcgccgcg ctggaggcag
     6301 ccctcgcctc gctggaaagc gggaggcacg ggctggcctt cgcgtccggc gtcgcggcga
     6361 ccaccgccgt gctgaacctg ctcgggcccg gcgaccacgt cgtcgccggc gaagatcttt
     6421 acggggggac gtaccgcctg ctcgaaaaat acttcgggcg ctggggcgtg aaaaccacct
     6481 acgccaatgc ggcggacccg gcgcaggtcg ccgcggcctt cacggagcgc acgcggctgc
     6541 tctggctgga aaccccgacg aatcccctgc tgcggctggc cgaccttcaa gcgctggcgg
     6601 aaatcgcccg cgcgcgcaag gcctgggtcg ccgtggacaa cacgttcgcc agcccctact
     6661 tccagcgccc gctcgaactc ggcgcggata tcgtggtcca cagcaccacg aagtacctcg
     6721 gcggccacag cgacgtgatc ggcggcgcgg tgatcgtttc agatgatgcg ttgcaccagg
     6781 atctcaagac ctaccagaac gccaccggcg ccgtgcccgg cccgtgggat tgctggctcg
     6841 tgctgcgcgg cctcaagacg ctcgcgctcc gcatgcgggc ccacgagcag aatgccctgc
     6901 gcctggcccg cttcctccaa ggccaccccg ccgtcgagcg cgtgtactac cccgggctcc
     6961 cggcgcatcc gcaacacgag ttggccaaac aacagatgtc cgggttcggc ggcatgatca
     7021 gcctggacct gaagggcggc ttcccggccg tcgagaaatt cacggccggg ctccagctct
     7081 tcatcctcgc ggaaagcctc ggcggcgtgg agtcgctggt gtgttatccc ccgaagatga
     7141 cgcacagttc cattccgcct gccgaacgtc gtcggcgcgg gatcggcgac ggcctggtcc
     7201 gcctgtccgt cggcctcgaa cacgccgacg acctcgaggc cgacctgcgg caggcgctgg
     7261 gaaccggctc cttctaaacg gccgccgaaa gcttgccaag accccgccgg tgggcaatct
     7321 tgatacagat gtcatgatgt ccgccccttc cagactccgg gttgccgtcg gcatgagcgg
     7381 cgggctggac agctccgtca cggcggccct gctggccgag cagggtcacg aagtaatcgg
     7441 cctgaccgcc cacctgtggc gcgagggctc ccgctgctgc tcgctcgacg acgcccgccg
     7501 ggcgcggcag gtcgcggagt tcctcgacat cccccactac ctcgtgaatg ccctcggctt
     7561 cttcggcgaa cgcatcgtcg cgcctttcgt gcaggaatac gccgagggcc gcacgccctc
     7621 cccctgcgtc cgctgcaacg aggtggtcaa gttcggcctg ctcctgcggg aggcgctggc
     7681 cctcggctgc acccacctcg ccaccggcca ctacgcgcgc ctggcccgcc gggaggatgg
     7741 atggcacctc tcccgcgggc gcgaccggcg gaaggaccag tcctattttc tccaccgcct
     7801 ggaccagagc cagttggcgc atgtcctgtt cccgctcggc gacctgacca agccggaagt
     7861 cgccgacctc gcgcgcgcaa agaatctgcc cgtgatccac gattccgaaa gccaggacct
     7921 ctgcttcgtg aacgccgatg gctacgccga gttcatcgaa aagcagcgcc cgaatctcca
     7981 tcagcccggc ccgatcgtgg acacggccgg gcgcgtcctg ggccgtcacg aaggttatta
     8041 tcacttcacc atcggccagc gcgaagggct gggcgtcgcc gccgcgcagc ggctctacgt
     8101 caaggaactc cacgccgaca cgaacacggt cgtcgtcgcc ccgctcgggg aaacgctggc
     8161 tcccggctgc cgccttgccg acgtccactg gatccgggaa gcgccgcgcg gaccgatcga
     8221 gtgcgccgtg caactccgct accgccacgc gggcgcgcgg gcgacggtcc ggccgaccgg
     8281 cgacggcggc gcgcaggtgg acttcaccga accgcagttc gccgtcacgc ccggccaggc
     8341 cgcggtgttt tacgacgggg acgacgtgct gggcggcggc tggatcgtcg cggaaaggac
     8401 tccatgacgt cgtggacaaa tatggcatgg cctgcggaac ttgccgggaa ggccgcggag
     8461 ctggagcgcg cgctccggga catgggctct gtcgtcgtcg cgtacagcgg cggtacggac
     8521 agcgcctttc tcgccgccgc ggcgcacggc gtgctgggcg aacaggcgga agcggtgacg
     8581 gcggactccc cttcgctgcc gcggaaggaa ctcgaggcgg cgcgcgaact ggccgcgcgg
     8641 atcgggatcc gccaccgcgt catcacgaca cgggaaatgg acaacccggc ctacctgaaa
     8701 aacgccccgg accgctgctt ccactgcaag accgagctgt tcacgctgct cggccacctg
     8761 gcgaaggaaa atgagaggag ggcggtcgtg gacggggtga acacggacga cctcggcgat
     8821 taccggccgg gccgcgcggc agccgggaac ctgaaggtcc gcagccccct gctggagacc
     8881 ggctggagca aggcggacat ccggcaggcc tcgcgcctgc tgggcctgcc gacggcggat
     8941 aagcccgcct cggcatgcct cgcctcgcgg gtcccgtacg gaacagctat caccagggag
     9001 atactatcca ccgtagagcg ggcggagggc gcgcttcacg aactcgggtt caacgtggtg
     9061 cgcgtgcgcg cgcacgggaa gtttgcgcgg atcgagctgg cgcccggcga catcgagcgc
     9121 gcggcccgcg agcccttgcg ctcggagatc gcgcggcgcg taaaggccgc gggattccgc
     9181 ttcgtggcgc tcgacctgca gggttaccgg accggaagcc tgaacgaaga cctgccgtcc
     9241 cggccgacat gaatctcatc ctcatcgaat ccccggaact gcgcggcgac cgggtccatc
     9301 tttcggacgc gcgcgccgaa cacattaccg ccgtcctcaa ggcgcggctg ggcgacgaga
     9361 ttcgcgtcgg cctgctcaac ggcaaccggg gccgggccgc cgtaatcgcg atcgaaaaag
     9421 gcgccgtcga gctggactgc cggttcgacg aacccgcgcc gccgccgccg cgagtggacc
     9481 tgctgctcgc cctgccccgg ccgaaagtca tgcggcggct gtgggcgcaa ctggcggcga
     9541 tgggcgtcgg ccggatcatc ctgaccaacg cgtggaaggt cgagaagatg tacttcgact
     9601 cgcacgtcct ccggcccgag gggtaccgcc ccccgctcat cgagggactc cagcaggccg
     9661 gcgacacgcg gctgccggac gtccacgtgt tcaagcagtt caagatcctg atcgaagacg
     9721 agttagacgg cttattcccg gacggcttgc gcctgctcgc gcggcccggc gagggatcat
     9781 ccgtcctgga cgcagtcccg gcggcgcggt ccggcagggt gctgctcgcc gtcggccccg
     9841 agggcggatg gatcccgtac gaggaggagt tgctgaccgc ccacggtttt cttcccgtat
     9901 ccatgggcac gcgtccgctg cgcacggaca cagcctgcct ggccttgctg gcgttggcgc
     9961 atgcgaaaca gcgccgggaa gcaggatatc acagtttata aactgtgata catttggaaa
    10021 cccctctatc ggccgcgccc gtagcgcagc tcccggaccg gcgcgtcggg cgaccacgtt
    10081 cccagcgcgg cgccgcgggc cagctgaacc ttgagccgcg cgccctcgcg gacgaagacc
    10141 ggggcatcca tgaggccgca cgcgatctcg acgtccgcgg gcccctcgtg gatcgtcggc
    10201 gacagcaggc gccgccagcg gcccgccggg aacttgacga cccgtttcgt cgcgccttcc
    10261 tggaagatcg gcgcgacgag caggtcgggg cccagcatgt actgggaatc ctccgtgagg
    10321 aaacgcgaat cgccggggaa ttccaggatc atcgggcgca tgatcggcag gccggtcgcc
    10381 gccgcctccc ggccgagttc gatcagcatc ggcttgaggt tcatccggag atgagccagc
    10441 agcctgtacg cggcctccgc ttcgtgcccg aacagccacg gctcgcgcgg cttgatgccg
    10501 tggtactgca tgaacgggct gaacgcgccg aactggagcc agcggatgta gagttcgggc
    10561 gaggggtcgc cgatgtagcc cccgatgtcg tggctccaaa acggcgcgcc gctcaagccc
    10621 gcgctcaagc ccgcgggaag caacgaacga tactgctccc agttggcgta ctggtcgcct
    10681 gcccagagcc cgggcgcgcg ttcgcagccc aggctggcgc tgcggcacca caacatcccg
    10741 tccacgccct cctcgtcgaa caggctgtag agggcctgcg cgtacagcgt cgtgtactcg
    10801 ttgtgcaact gccagccgcg ccggccgtcg gcaaagacat cgtcggcctt gatgtcctcc
    10861 ccgtcgtccg ccttgaaccc tcgcgcgccg agccgcacgg cgtcccgcat ctggtccttc
    10921 caccagacca ccgcttccgg cctcgtgaaa tccacgttgg ccatgccctc cagccactcg
    10981 cggcgccaac ggaccgaacc gtccggcgcg cggacgaagt acccggaccc ggccaactcc
    11041 ttcaggccgg gatgcgaggg gggaatgacg ggaacctgcc agagcacgtt gcggatgccg
    11101 tgctcgcggc agaacgcgaa gtactcgtcg acgttccagc cgccatgcgc gaagcggttg
    11161 aattcccccg tttcgtaccg gcccttccag gcctccagga cgatggcggc cacgggcagg
    11221 ccgcggtccg tcatgccgcg aatggcctcc tcggcctcgc cctggttctc gtagctgttg
    11281 cggctgatcc acggccccca cgcccagtcc ggcacgccgc ggaacgggcc ttgaatggac
    11341 gcgcgcttga gcacgagctc cttcagcgtc ggggcgaagg cgaggtagaa cgtccattcc
    11401 cgaccggctc gcgaataccg gttccatccc tcgcccagcg ggttgaggga gaactcgccc
    11461 tcggggttgt cggcggcaaa cagcgcatac ccggaactac tgaacagcac gggcgcgcag
    11521 tagtagctcg cctcgccctg ccccgggacg tcgcggatcc acatgtccac aacttttccg
    11581 gcgacatcga gcccgccgaa ccgctgaccg agcccgtaga gcgcctcgcc cggccgcgcc
    11641 acccactcca gcctcgagcc cggcacatgg gcgcggccat aggcttcaag cgaagacagc
    11701 cgcccgcgcc acagcggagc ggcgccgggc gcgtccggcc gccggaattc cagccagccg
    11761 tccgccacgc gcacgccggc cggcgcctcc tgttcgggcg ccgggtcctc accccggggc
    11821 cggaccgtga ggcgatagag atcccgccct tcggcggcga ccgtgacgga taggtcgctg
    11881 aaagcaaacg gcatcatttc ggcgcccggc gccccgcccg agaacaggag cagcgcgacc
    11941 gctgtttttc cgatggttgg aaaggaagtg ttcatacgtt tccaatgcct ggaaaaaccg
    12001 ccgccatttt ttccaaacac ggggaaaaag gccagcctgg ttttcgccgc cgctcaagcc
    12061 ccgagcccgg cgaggggcgg agcgcgaagg ctggcggaag gggtgggacc atctacgcct
    12121 cgcgcggcgc gttggcttcg atggtcacga ttcgaacccc tccaggacat gcttcttgag
    12181 aaacgccttg ccggacgggg tcttcaagta cctctcaaaa gccagggcca cggaccgccg
    12241 ggcaaaagca atgcacgcga tccgttccca cggcgcggag cgactggagt acgtttgcgt
    12301 gaaagtgtta tgctcaacca gccgttcgtc cggtcgcgtg ctgaatccca cgtaggtctt
    12361 ttccggagcc accagacttc gcagaacata gacaaaatac tgctccttca cgccccctcc
    12421 tctcctgcga agccaatcgc agccacgagt tccggcgccc cgcggcggaa cgagggcgga
    12481 gattggcgga aggggtggga ttcgaaccca cggagcactt tttgggcgct ctcccgattt
    12541 cgagtcgggt gcctttaacc actcagccac ccttccaagg gaaacccgtc agacggctgg
    12601 caggctttct ttacgtcgca tatcccggcc gccttgtcaa tgtccgcccg attggttatg
    12661 atggatgacc ggaaacaagg acgcgcgccc atgttttttc agcgagccgt catggccgtc
    12721 acgctggcgc tggtggttgc cggcgcagcc cggggtcagg acatccggct ggcgaacggg
    12781 atgcccgtgc ccggcacgct cgtccaggcg aagccggaag gcctggagat cagcaccccg
    12841 accgggccgc gggtgctgac gtgggaaacg ctgtcggccg gcacccgctt ccgcctccag
    12901 gaatcctacc gcatcacgtt ctcgaacatc ctggaaggcg cgtccgtcgc ggagcgggcg
    12961 gcgctgtacg aagcgcggaa ggccgcgccc gcgcccgaac cggcgccgga accgaagaag
    13021 aagacgcgca agaagaaggc cgcggagtag cgcccctgtc cgccgcgggc ggggcttttc
    13081 actgcgcaac aaattgcttc acatccgtga atcaatttgt tatcggcgag gcgcgggaga
    13141 ccaacgggac cggagcgcga acggcggagg ccggttcacg ggtagagatc gagcagcgcc
    13201 ggcgcgatgt cggtcagggc gcgcacgcgg atcttcaacg cccccgctcc cgccccgagg
    13261 gcgacgaacg ggaccggatt gcccgtgtgc gtccgcgtgg cggcgtcctc gatgttcccg
    13321 tggtcgctgg tgagcaggag caggcggcgc tcgccttccg caaaggcaag aacgccgccg
    13381 agaaactcgt cgaattcccc cagcacgcgg cgggtcctct ccgcgtcccc cttgtggccg
    13441 gcgcggtcgg tctggaagta ctcgaagagc gtgaagtcgt gctccgtggc gagcgcggcc
    13501 aggtcgcggc cggcctgggc gggcgtgacg aaggggccgt cgtatccgcg ctcgcgcagg
    13561 ctagcgcgcg tcaggtcctg gtacaccgcc cgcccggcga gcaggtccgc cgtgtcccgc
    13621 accgcgccga aggccgccag cgtggccacg gtggtcacgg actggaagcg gcgcgagcgg
    13681 acctcctcga tatcgcgggt gtagtaggcg ttggcgaagg tcgcccggta gccgcgcgcg
    13741 gcgagggcgg agagcaggtt gtgctcccgg atgatttcct tcaggcgcgg ccccggcagg
    13801 ccctcgatgt gccggcccat gacggcgggc gcgttgacgc cggtgagcag ggcggtctgg
    13861 ccggtagcgc tctggggcag gcccggcacg ccgaggccgg cgtcgatggg cgcggcttcc
    13921 tcccggagca ggcgggccag gcgggggcac gcgccggaga agagcgggtt gtgctcgctt
    13981 tccgggccga ggccgagccc gtccacaaaa aggagaagag tttgaacgac cgattccacg
    14041 ggggcaccat accatcccgc gcccgggacg gctacggcgt cgcggagttc cgcccgccga
    14101 gggcgatcag aaaaccggca ggttgggtag cgcggccggg agccgggtct gcaccggcgg
    14161 gaccaactgc tggaactgcg cgttcgcgcg cagcccgttg aaccgctggt ctttccggat
    14221 ggcgtcgcgg gcggcgtcgc cgccgacggt gatggcctgc cggatggact cgaggcacgg
    14281 gtccgtctgc tgcagggcca gctggacggc ggcgaggtcc atccacacgg ccggctggga
    14341 cggatcgcgc gcgacgtagc gctggagggc gacggccagg aggtccgcgc ggcgcgcctc
    14401 ggcgaagatc cgggcgatct cgaggtagaa ggcggggggc acgctcccgt cgttgagcaa
    14461 ctgcatggtc aggccgacga actgctggtc gaggttgagg cggcggtaca actgcgccag
    14521 ttcgagagcg gattgcaggt cgccgcgccc ctggttgaag gaggcctcga gctcgttgcg
    14581 ccgggcgtcg tccttgtccg tgctggagat ctggttgagg aaatcctgca gcttgtcgtt
    14641 gcccttgtcc ttcttgagga agttctcgat cagctcccgc gcctcggcga agcggcgctg
    14701 ctgcatcagg atgtcggcca gccggtagtt ggcctcgggg ctcaagtcgt agaggtcgat
    14761 ggcctggcgg aaggcgtact cggcctcccg gtacatctcc tcgcggcgcg gggtgtcctg
    14821 ggcgccgcgg aaggcgtaca ggccggcgat ggcgctgcgg agcttggaga aggtcttgcg
    14881 cgcgacgacg tcgcggtcga atttcgggtt cttgagcagg tagtcgcagt accaggtcca
    14941 gaaatcgtgg tccttctgca cgaggtcggg cggcagctcc ttcatcggct cggcgttgat
    15001 cttcatgatc aggccgtgcg gcgtcaggta ggggtacatc caggggatga cgtagctttc
    15061 ctcgacgtag aactcgtgct tgtacttgtt ctgctcgaag atcatttggg cgaggatgcc
    15121 gttgatcatc atcacgccgg cgacgccctg gacgctgacg cgtccgttct cgacggtcac
    15181 ggcggcgccg gcggggacgc ggccggcctg gacgtcgtcc acgtagcgct ggaaggcgag
    15241 gttgctgtcc tgctgggacg ggatccagat cgtgtcgccg tagaggtcgc gcatgacgtt
    15301 catgtacgta ttgtcggcga gcgcgttctg ggtgatgagg tagacgtcgc tgcggcactt
    15361 ggcggagtag atcatgtagg tcggcacgaa gcggccggga tccgtgccgc cgaagaagat
    15421 cgcgttggtg cccatctcgg gcgggtagtc gggggtcggg tagctcttcc agatctcctc
    15481 gaattccgcg tccgagcgct tgaagtgaag gtcgtcgcgg atgccgttga cgccgcgcag
    15541 ctgccagtgg ccgaactgcc agccgaagtc gtgcccgttc tgctcggcgc cgccgaggat
    15601 caggagctgg ttgtcgtcgt agccgttctg gtagacgagc acgagcggca ggagcacggc
    15661 gaacaccggg ccgaggaacc gcgcgatgcc catgccgccg aggagggtct ccagccaggc
    15721 gaggacaaag atgagcccgt acccgagcca gatggcgtac accgcgtggg actggatgaa
    15781 ctgcacgcgg ccgatgaaga gcgtctggat gtccagcgcc gggttctgga agatgacgag
    15841 gatgatgctg acggagaaga aggagatcag ggcggtgatc agccacgtgc gggcgcggcg
    15901 gtcgagcttg atgaagaaca cgaagggcag cacggccagc agggagatcg gcgaggagaa
    15961 ctgctcgcgc aggtccgtga tgtaggacag gatctgctcc aggaacttcc tgctgaagat
    16021 atccgtcggc gtgatgcgtt cgtactggcc gcggctgatc gcgtgcttga agccctccca
    16081 ggtccgcggg tagccccagt tcatgggcgg gttctgctcc gaggcgaagg gcatgtaggc
    16141 gtagaacgag aggccggcgc cgacgagggc gacgaggatg agcacgcgct tccattccgt
    16201 catcagctgc cgctggatga ggaacaggcc gacgggcgcg aacaggaaga ggagggcgaa
    16261 ggtcagcgtc aggtgcttct gcttcaggac ctcggcgggc gcgttggggc cggggttcat
    16321 gccgtacgcg gcgaagaaga ggtacgcgga gatgcacagg aacaggccgg ccaggcagtc
    16381 gcggaacagg gcccggtcgc ggaaccagag ggcggccatc aggcccagca ccatgaagag
    16441 cagggtttgg tggttggtca ggcccagccc gaagatgaac gcggtgatgt acagcgtgtg
    16501 gtcttccttc ggccggcaca tccagcggta catcagcagc aggaggagcg tctggaagag
    16561 ggcgttgagg ctgtacacct cgacgatgac ggactgcgac cacatgaccg ggctgaaggc
    16621 cagcagcagc ccgctggaca cgcccgccgc ccagcacagc acggtttccg tgcggtagcc
    16681 gaggacctcg gtggcgcgct tccaggtgcg gatgatgtcg ctgccggacc ggctgacgag
    16741 catggccagc agggcgcagg ccagcgcgcc ggacgcggcg gagaagaggc cgacgctcca
    16801 ggcgggattc gggaagccgt agtacgtgac ccagtggaag atccactgga agaaccaggt
    16861 caggagcgcc cagatgggat agcccggcgg gtggggcacg cccaggtaat cggaagcgac
    16921 ggccagctcg ccggagtcct ccagcgtcac cgtgggggcc agggtgtaca cgtagacgcc
    16981 cagcgtcacc aggagcacgg tccagaatgc gctccagtcc gcccgccgga agaagcggcc
    17041 ctcctgctgg tcgaggaaat ccgggcggtt ctgttccggc gcggcgccgg ccggcgcgcc
    17101 gggggccgcg ccgccctgct gctggttcca ttccgatgat gtcacgtgtt cccgtccccg
    17161 gtgaaaaacg ccgcaaacgt taaggttttt tgcgcccgcc cgtcaagccc cgtcgtgatt
    17221 ggacagccgg ccgggaaaaa cgttgcgccg cgggatgaga aagcgctcat ccgtgcggct
    17281 cgatggccat caaggggtag agctggctca cgaagttccg gatctgctcc cggtattcat
    17341 cggagtcggg cgtccggatc ccggtcaccg cgatgtacgc ccaccggtgg gatacgctgt
    17401 gcaagatgcg gtcggtggcc atccacatca tccgctgcca atggtgcggg gtctcccgcc
    17461 ccttcccgac gaaccagtac gcgtagtacg agggatactc gaccacccgc tggtccgcgg
    17521 tcatgtactg gcggagcatg tcgatgacca tgacccggag ggccgcccgg ttcgcgatcg
    17581 gcacctccag cacgcggctc cccagctgct tgttgccctg cccgcgcagg cagacctcgg
    17641 gccgatggat gctggcccgc tccttgccgc tcaacacgat ggacacgaac acggcctggc
    17701 ccatcgggtg ggtgtacttc ttcttcagga cgatggtgtc cggaggcaac agggtctttt
    17761 cgccgaggct catcggggcc agcgggctgc cgcacagggg gcagacatcg ggattctcga
    17821 gggcgctccg cacaaactcc ttctcgcagg tcgggccctg gcagtagcgg atttcctcgc
    17881 cgcgccacgc cccgacttgg tcgggcaacg ccacgctgat gccggcctcg tcggtgatat
    17941 tgacgtccac cgtcagcgcc agcgccaccg acatcgccac gaagagggcg atcaggacga
    18001 aatagggctt aagagttcgc ttttccatcg gagccagatc tcccgcgggt ggtggttcag
    18061 caggccgccc aggccgatca gcaggccgag cgagacgggg aagaaaatga agccggaata
    18121 gtcgtggtag aggccgacgg cgacgtcctg cccgaaggcc tgggccatga ggctgatggt
    18181 gatgatccgc atgacgttgc cgacgatggc cagcggaacg gacgccagga aaaggatcca
    18241 cttccggacc agggtcttct gcgtgaagta ggcatagacg gcggacagga tggtcagcgc
    18301 catgatggag cggagcccgc tgcacggcgc cgccacctcc agcgtccagc gcaccatgtc
    18361 cggcccgtcg ttgaccgtca tccgcgtgcc cacctgctgc acggcgatgc ccaggccgtt
    18421 caggaggtgc gtggaggcgg tcgtcgccag aaggctcaag ggcgccgtca gctcgtccag
    18481 gaagttcagg ggaatgcaga agaacaggaa cgccacggga aacacgaggt gcttcatcac
    18541 ctgccagccg tacaggtaga gcggcgcgcc ccagagcagc aggaccaggg cgaagaggga
    18601 cacgcgcacc tgctgggcct tggcgcccat ccagtgcacc agcagggcca gcacgaccag
    18661 cgcgagcccc caccggttcg tccgctgcgg ggcggccgcg atctgcttgc gctggcacca
    18721 gatcacgccg gccgagatga acgggatcag ccagctgtac gacaggtcgt cgtgccagta
    18781 gctgaccatc cacagaatgg ccgagcggcc gaacatccgg atgtccgtgg tgttgccctg
    18841 gagatggaac agcaggtaga gcaggccgac caggatggaa gcgagccccg cccgccgcag
    18901 cgcgtcgggc ggcagggccg ccaggcgcca gcgttcgctc cataactgcg aggatgttcg
    18961 agtgtaggac atcgccggga acaacttggt cagtacccta ccccgttttg ttcagcgggt
    19021 caatgaccgg ggccgcgcgg ttagaatccg gttccccgga tcacggccag gggcgtcttg
    19081 accaggatgg ccaggtcgag gcccagcgac cagtggtcga tgtactccag gtccagccgc
    19141 atccagtcct cgaacgacag ggcgttgcgg ccgctgacct gccagaggca ggtgatcccg
    19201 gcccgcatgc tcaggcgccg ccgctgccag ttctcgtatt tcgccacctc ggcggggatc
    19261 ggcggccggg gccccacgag gctcatgtcg ccggccagga cgttgaacag ctggggcagc
    19321 tcgtccagcg agtggcggcg caggaaacgg ccgaccgggg tcacccgcgg gtcggcccgg
    19381 atcttgaaga cggggccgct gacctcgttt tgcgcctcga ggcccgcgcg ctgctgctcc
    19441 gcgtcggcca ccatcgtgcg gaacttgatc atcgtgaaga cgcggccgtg cagggtgcag
    19501 cgcttctggc ggaagaacac cgggcccggg ctggtcagct tcaccgccag ggcgatggcc
    19561 aggagcaggg gcgacagcag ggccaccagg accagggccc cggccatgtc catgatccgc
    19621 ttcgccacca gcgcccacga cagcgccggc gtgctgcgga agaccaggac gggccggttc
    19681 tggaactggt ccatgtgggc ggtcgcgatg ctggtccgga agaagtcggc gagcagccac
    19741 gcctccacgc cctccgtttc gcacgtctgg atgagttgct ggatctcctc gaagttctgc
    19801 cggccggggg ccagcaggac ggaatccaca gaatgctcgt gcaggatacg ggacaggtcc
    19861 tggaccgcgc cgaggatcgg cagcccagcc ccgtcgcccg cgggttcgcc tgccggcgtc
    19921 agcacgccga gcagccggac ctgccaggcc ggctgctgct gcaggtagcg ctggaccgac
    19981 tcccggtccg cctgcccgcc cacgaggagg atcgaggccg gcgaactcca caggggaacc
    20041 agcttgctca tgatcgcgtc cttgatccac agcaggatga cgtccatcac cccgaagatg
    20101 atcaccagca cgcgcgggat atgcttgacc ttgagcaggt agagcaggaa gaacagggtg
    20161 accacgccga tcacgttggc gcgcgccagg atcttgaggg aatccaggcg cgaatgcgcg
    20221 aactggtgct ggtagtagcc gttggactcc aggatgatga tccacagcgg cagcagcagc
    20281 aggtacagcc acgcgtgctg ccagagggcg tcgaacggcg ggaaggtgtc cggccgccac
    20341 atggtcagcc agcgccgcga cgccagggcg aaggtgaagg ccgcggccgt caccagcgcg
    20401 tcgagcaact gggccagctg ggaccggaac tgggatgtcc tgtagagcat gcccgaccgc
    20461 ccgcaaaaaa agactcaccc gcgggcgatg aacgtgcata atggcgcatg ggattgcgtt
    20521 tttcaatgct tctttgggcg ggatgaacca tgcgcatcgc catgatgggc ctccgggcca
    20581 tcggacaggg ctcgggcggg gtggagaagg ccgtcgagga aatcgcccgg cgcctggcgg
    20641 cccgcggcca cgccgtcacg gtgttctgcc gcgccaggta taattccgcc tgcgccaagg
    20701 agttcgaggg cgtccgcctg gtcaacctgc ccgccgttta tacgaagcac ctcgaggcga
    20761 tcacgcacac gttcctggcc gcgtgccggg cgctggccgg ttatgacctc gtgcacatcc
    20821 acgctacggg gccttccatg ctggcattcc tgccgcgtct cgcggggcgt cggacggtcg
    20881 tcacggtgca cggcctggac tggaagcgcg aaaaatgggg tgcgggcgcg cggctctttc
    20941 tccggctcgg ggcctggacc gccggggcgt tcccgcaccg cacgatcgtc gtgtcccgcg
    21001 aattgcagcg cttctaccgc gagcgctacg gccgcgagac cgtgcctatt cccaacggtg
    21061 tcacgccggg cgtccggcgg ccgctcgacc gcttgcgccg gttcggggtg cggccggacg
    21121 agtacctgct ctacctcggc cggctcgtgc ccgaaaaagg cggccacctc ctgatcgagg
    21181 cgttccgggc cgtgccgggc gacctgaagc tgctgatcgt gggcggcgcc agccacagcg
    21241 aggattacct tgcccggctc cgcgccctgg cggaaggcga cccgcgcatc gtcttcgccg
    21301 ggccgctgta cggggaggaa aaggaagagg cctacagcaa cgcgcgcgcc ctggtgttcc
    21361 cctccacgct ggagggcatg ccgctggtgc tgctggaggc gatggccctc ggctgcccgg
    21421 tgctctgcag cgacatcccc gagaacctcg aggtggtccg gccggacggc gcgccgggct
    21481 ccgcactcgc cgctcttttc ccggccggct cggccgacgc cctgcgggct gcgctcccgc
    21541 cgtttctatc ggcgcccgaa gccgcgcgcg cccgcgccgc gcaggcggcg gcctacgtgg
    21601 cccgcaccta cgactgggac cgcatcacgg acgccacgga agaagtgtat cgctccctgg
    21661 cggcccgcgt ttgacgcccc ggcccgattt ccacactttg caaagtgtgg aaatcggagc
    21721 gcgggcattt cggcatcgtt ttttacgtga atcgtgtgca tcatggatca atacgctatg
    21781 aaaaggtttg ggcgaatccg gcgcggcctg gcccttctgc tggcgctcct gcccgcggtg
    21841 gcgcgctccg gcggcgggcc gcagaacgtg ctgatcgtgg tcaacgacgg cgatccggac
    21901 tcgcaggaac tgggccgcta ctaccgggaa gcgcgcggca tccccgagcg gaatgtctgc
    21961 cacctgaacc tgtccgcccc gctgcataac accttcctcg atatcttcga gaaatccatc
    22021 cgggacccga tcctggcgca tatccgggac gagaaactgt ccgaccagat cgacttcctc
    22081 gcgctctgca tggacacacc gacccggatc aacgacagcg agagcatcac cgccgccctg
    22141 ttctacggct tcaagaacgc cccgccggcc ccgccctgcc agttgtcgcc ctcaacgttg
    22201 agcttctatt tcaacgccga gcgcgccttc acccacgacc tggcgggacg ggaaaacccc
    22261 cactacctgg ccatgatgct gaccggcccc gcgccgcggg acgcccgggc cctcgtggat
    22321 cgcggcgcgg cctgcgacgg caccgcgccc accggcacgt tctacctcgt caagccgccg
    22381 ggcgacccgg atcggaacat ccggtaccgg ctcttcgacg agctggattt ccacgcgcgc
    22441 ttcatgccgg ggttcccgcg acgcgtgttc tcggaggaga acacgctcac gggcctgacg
    22501 gatatcctcg gctacctcac ggggggcccc aaccatcccg agaccttctg gaccgcgaac
    22561 cagtacctgc cgggcgcgat cgccgaccag ttgacgtcct tcagcggcct cctgccggtg
    22621 ccgcccatgg gccagggctc gatgctccag tggatcggcg ccggcgccac ggccagttac
    22681 gggacggtca gcgagccgtg caacttcctc gaaaaattcc cgtctccgat ggttctgttc
    22741 tggtacgcgc ggggcttcaa cctggccgag agctactgga tgggcgtcgc gcacccctac
    22801 cagggcctct tcgtgggcga tccgctcgcc gcgccgtatg cccgaccccc gcgcgtcacc
    22861 gtcgccgcgc cggcggccgg gcagaccgtg tcgggaacgg tcaccgtgcg ggtcagtgcc
    22921 gcgggcaccc ccgagtgtcc ctgcgcggcc ctggacttct acctggacga cctccgggtc
    22981 ggcaccgtga ccaacatacg gccgcaagcc tggaatgaag tctacctgga gttcgacggc
    23041 caggaataca gctatatcct cggtgaatcc gacgacctcc cggcggccgt ccgcggcctg
    23101 gcgcgggagg cccggaaaaa ccccatgctt aacgccgtcc ccttcggcga ccggctgatg
    23161 ctgatggacg ggcgcatcgg gaaacgcggc tccaccacgc cctacggcgc gcgcgtcgaa
    23221 caggggctcg ggccgccgcc cgggttcggc gcgcgcaacc tgaccccggc cctgctcgac
    23281 ggaacctacg cggcgaggaa atatgtcatg ctccagggcg cggccgccgc cggggacgcc
    23341 gttgagtgcc gggtgacgct gggcaacggc ctgatcgcga cggcgcgcgt ggaggccgcc
    23401 gagggcgagg cggcgctcga cgtggcgaaa cgcctgaagg cggccctcga cagtcatccc
    23461 gtcctggccg gcccggacgg aatacggatc gcggacctga aggccaaagc cgatcgcgcg
    23521 gacttcgccc tcgagtcgag gacggccggc ccgccgggcg tggccatcct tctccgctat
    23581 gccatcctgc cgaagacccc gggcgccggg ctggctccgc cggtcgccta cgaggggcgg
    23641 ctctcggaca acatggacct gatgagttcg cggggcatgt tgctgttcca ttgcggccgc
    23701 gagaccctgg ccgcggagtt tccatgggac acttccggca tcccggacgg gccccacgtg
    23761 ctgcgccttg tggcccgcga cggcaccgcg gtgcagaccc agggccacgc gtttgtcccc
    23821 gtcgtcgtgc gcaacacgga ccggcgctgt cggctgaccg gagtggagtc cggccaggtt
    23881 gtccgggccg gcgcccggct ttcgctcggc gtggagtgcg agcccgccga cttccctgtc
    23941 ggcgcggtgg acttcctcgt cgagggaaag atcgcggatc gcgcggggaa agcgcctttt
    24001 cacggcacgg tggacaccgc cccctaccgc ggcggcaggg tgagcgtgca ggtccgggtc
    24061 gcggacgcgg acggccgcca gacgctgtcc gaccccgtct ggatcagcgt gccccgctga
    24121 acccgcgccg cctccttcgg ggcttccctc gcgggtttcc ccggggtatg tttataaaag
    24181 gtgcgatcat gaggatcagg gtttcagcct tgctggccgc ggcggcggcc ctgctgtccc
    24241 ctctcccggg ccgggccggc ggcggccccc agaacgtcct ggtcatcctc aacgaccaga
    24301 gcatcgaatc gcaggaaacc ggcctgtact accggcagtt gcggggcatc ccggaacgca
    24361 acgtgctgca cgtctccgtc ccgccggcct ccgcggcggc ggggttccac gacatgacca
    24421 acaccgcctt ccagacctcg atcgtggacc gcatctcgca gcacatcggg ctgtacgggt
    24481 tgagcaacca gatcgacacc ctggtcctct gccaggacct acccacgcgc gtcagcgata
    24541 acgagggggc cacggccgtc cttttctacg ggttcaagaa cgcccccaag atcgtcgctt
    24601 cctgcagcat gcccaccaat acggcgagcg gctactacaa ggccgagcgc gggttccgcc
    24661 acgcggacaa atacgcgggg acgaattact acttgagctt catgctgatc ggcgaccgcc
    24721 tctccatcgc gaagagcaac gtggacgtca gtgtccggtc cgacgggacg aggccggggg
    24781 gttgcttcta tcttgtcgag agcagcgacg cggagcgcaa cgtccgctac ccgctcaaca
    24841 acaacttcga ttttcacgcg cgtttcctgt cgggcttccc cgcccacgag atcacggggt
    24901 acggccccct gaaccaggag acgaacgtgc tgggcttcca gtggggctcg tcgcagatca
    24961 acgactactg gggcgccacg aaccggtacc tgccgggctg catggccgac cacatgacgt
    25021 ccttcggcgc gcggttcccc accgacccgt acaaccaggg gaagatctgg aactggatcc
    25081 ggcagggcgc gtccgccagc tacggcgccg tgaacgagcc ctgcgcctac atcgagaaat
    25141 tccccgatcc ccttgttttc ttctggtacg cgcgcgggtt cagcctgggc gagagctact
    25201 ggatgagcgt cgccaacccc taccagggcc tcttcatcgg cgacccgctc gccgcgccct
    25261 atgccgaccc gccggcggtc gccgtcgcgt ccctcgcctc gaaccaggtc gtgtcgggga
    25321 ccgtcgccgt ggacatccag gcgcggacca acgccgcggg ccggccggcc gcccgggtgg
    25381 acgtgttcgt ggacgacctg tacgcgggga cgcccaccaa cgtccccccg cggccgggca
    25441 acgtgctgca ggtgacgatc tccgggcagc ccgtggccta ccaggtcgcg gccggcgaca
    25501 cgctgtacag cgccgtcacc agcctcgccg gccgggtgaa cgcgagcaac ctcgccgtgc
    25561 gggccgcggc ccgcggcgac cggctggaac tggtgtacac caactacggg tacttcggga
    25621 cgggcgtgac gtactcggcg gggacggaga cgggcgcggc cgccgagttg acggtcttcg
    25681 cgcaggcgct cggctccaac ctgctcgaga gcgcgtacgc cgcgcgggaa ttcgtccagc
    25741 tccaggccta caacgggaag gccaacacgg gcgacacggt gacctgcgtc atcacgctga
    25801 cgaacggggt cgcggccacg aaccggatca tcgccgccca gaacgagacg cccaagcagg
    25861 tcgtcacgcg gctcgtggcg gccatcaaca gccacgccgt cctgcagggc acgaatggag
    25921 cctgggcgaa cacgcacacc taccaggagg tgtccagctt cccgctcgag gcgcgccgcc
    25981 ccgggccgga gggcatccgc ctgcacgtgg cctacacgat cgtccccgcc atcccgggct
    26041 cgggcctgta cccggccgat tcgttctcgg actttttcaa cgacaacgaa agcgacctga
    26101 cggcgcgcgg caacgtgctc ttctggtgcg gacggtcccc gctggacacc tcgtgggcgt
    26161 gggacacgac ggcctggccc aacggcccgc acacgatccg cgccgccggc cggctcggga
    26221 ccggccccga ggcccagggg cacagcgtga cgcccgtgct ggtcagcaac tcggccttcg
    26281 cggcccggct gctttcgccg accaacggcg agccgttcgt tctgggcagc aatgtgtttc
    26341 tccaggccga ggtcaccaac gcgcccggcg tcgtcacaca ggtggtcttc tacgtggagg
    26401 acaagccctg ctccgtgctg acgaacgccc cgtgggtgtc ggcgttcgac acggccgacg
    26461 ccggcgtcgg cgcggtgtcg gtgaaggccc tggcgtggga cagcggcggg acccgggccg
    26521 tgtcgccggc ctcctggatc gcgatccagc gctcgcccac gctggacacg gacagcgacg
    26581 cgctgccgga ctcctgggag cagcagtact tcggagggct gtacgtctac gcggccacga
    26641 acgacccgga cagcgacggc atgccgaacc acttcgagca ggcgcacgcg ctgaacccta
    26701 ccaacaacga cgccgcggcc gatggcgacg gcgacaaggc cgggaactgg caggaatacg
    26761 tcgcggccac ggacccgggc gactccaaca gcgtcttccg ggtcgccagc ggctcggaca
    26821 ccaaccgccg cccgctgctt tccttcttcg ccgacaccgg ccggctctac gaggtgcgtt
    26881 acaattcgga ctacctgacc aatttcaacg gatggctggt cgcatccccg gcccccttcg
    26941 cagggagcgg cgggctgacc aactggctgg acgacggcag cgagaccgag ccgccgccgg
    27001 aggacgtcac ccagcgcctg taccgggtcc gcgtcgcgct gcccgactag ccatgaaagc
    27061 cgaacctccc tccacgagcg cagcccggct gcttcaggcc gatcacgcca aggggatcgc
    27121 catcgcgctc gtggtcatct gccacgtcga gcaggggctc ggaagggccg ggattgcggg
    27181 cgtccccgcc ttctggccgg ccttcgaggc gctgtgctac acgttcatgg tgccggtgtt
    27241 cttcgtgctg tccggtcact tcctggaagc ctcgatccgg cgccggggcc tgaaaggcgt
    27301 ggccgcgagt ctcccgggga cgctgctcta cccctacgtc gtgtggacgc tcctgcaggc
    27361 cgcgctgatg atcctgaccg gcgccggcaa ccggcacgtg gcctgggtgg aagtcccggg
    27421 cctgctgctg cgcggctgga tgcagttctg gttcctccac gcgctgatgc tgatctacgc
    27481 cctcgacctg ctgctccgcg gcatgcgcgt cgggcgcttc ggacgcctgg cggttgcgct
    27541 gctcctcgac ggcgcggcgg cgatgcgccc cgactggttc ggcgccccgg tccggctggc
    27601 ctgccagcac tatctttttc tggccctcgg cgcgctggcc ccggagcgct gcttcgaagc
    27661 gctgccccgg agacaggcga tcgcgctggc cgtcgcggga ttcgcggggc tctatttcct
    27721 gcacgcgcac cgggcggcgt gctccgcgct gctgctcccc gccgccacgc tcgcgggcgt
    27781 ggcggggacc ctggggctgt catccctgct ggccgccgcg ccctccctga acgggtggag
    27841 cagcctgggg cgttattcgc tcgaggttta ttgcgcgcac gtgatcttct ccgccggcac
    27901 gcgggtggtc ctccagcggg gcctgcacgt ttcgctgttc tggccccacg aggtggccgg
    27961 ggtggcggcg ggcctgctgc ttccgctggg gctggcgcgc gtggcccgcg cccggggctg
    28021 gccgctgttt cggcccccgc gccgggcccc gtgacgggac cggccggacg ccgggcatca
    28081 gaaccggaac ggcatccgga aggacgagcc gacctgcagg gccagttcat cctccttccg
    28141 ttgcgtgggc gcctccggcc cggacaccca atggataccc accaggctcc agagcggaag
    28201 catcagcatg ctcaagttga aggtttcgat cagcatcccg acgagaaaga ccgtgatgac
    28261 gcctccgatg atgccgagcc gctgcccggt ttcgaacgag aacccgggat cctggaggca
    28321 gagcctccag agatggcgca tggcggcgaa ccagaaccag aagtagaagg ccatggagat
    28381 cggcccgaac tcgccccaca gcgtgtagag cgagctcatg gcgctcccga tcaccgaggt
    28441 ttcggcggct tgcccgcggc tccggaacgc ggtcaggacg tagggcagca cgtacttgtt
    28501 ggccaacggc cgcatgctgt acgtgcccac cgtgctgcag aaattgccgg ggccggcgcc
    28561 cagcaggccc agcaggggca cctcgccggc caggtcgcgg gtgatcgttc ggaacacggc
    28621 gcccttgccc gagtacttca tagagtgcat caccatggcc gggcgattct cccaggactt
    28681 cgtggcaaac gacaggtaga cccagctcgc cacaaggaag accagcatag ccagcccgaa
    28741 cagcacccgg ttccgtatcc cgatgcgcgg gaacaggaac atgggcgccg ccgcgagggg
    28801 gaccatcagc agcacatgct tcgtatcggt caggaaaatg aggtcatacg cgatcagcag
    28861 cgccatgaac agcgcgagcg ccacacgctt ccgcgggacc ggcggcttca gcgagaacag
    28921 ccagcccccg agcaggaaga gggcgatgac ggacatgtac cccacgatgt gcgcgctgcc
    28981 gatcgatccc cggctgtcat ccgccgtgag gcggatcgac aagcctttct gccagactac
    29041 attgaagggg agctgaagga acgccagggc caggagcatg atgaaccaca gcttcttttc
    29101 ccgcggcgac cagggcccga tggcgcgggc atagaaccag aagatgtaga acttgccgta
    29161 cgaaagggtc acgcggatga agttgagggt cgggacgtgg ttgaccttcc agctcaacag
    29221 cgccaggccc aggtacagga ggggaaagaa cgtcgcgccg cggggcagtt ggcgatggat
    29281 cgccttcatc acgatgatcg ccatggggac cgccagcagg gcctcgtcga ggatgccgat
    29341 gaacgggatg gggacataca ggcacccgaa gaacaggccg ttgaacgaca cggcgtggta
    29401 gaagaagacg aagtagaggt acagtttcgg gtgttccagg aagaagacgc cgaccacgaa
    29461 cagaatgacc agcaccgacc acatgagggc gggcgagaac ccgaaaaagc cggccaccca
    29521 gtacgaggcc accagcccta tcacgaatcc cgcgatgccc agcagggcaa tggggacgcg
    29581 gactatttta tctgaagcca tggttgccgc ccggcacgcg cagaaaacgg gccgaaagcg
    29641 gcgtaaaccc tatcacatcc cgcaagtcgg gcaagagtta tccgcatccc ggccgcacgt
    29701 ccggggttgc aaaaacacga aggcctgaca gtatgaaatc atggttttgt ttggccagcg
    29761 gccgggccgc ctcggcgccg ccctgctgct cggcgcgctg gcgcttccgg ccggggggca
    29821 ggaggcggct cgaatcgcct actacccctt cgacggcgac gcgacggacg gcagcgggaa
    29881 cagtcgggac ggcgagatcc ggggcgccgc ggcggccgcc gaccgattcg ccttccggga
    29941 ccaggcgctc cgcttcgacg gccagaacga ctacgtcgcg gtgcccgaca ccggcctggc
    30001 ggtgggcggc gacttctcca tcgccttctg ggcgcgatcc atcggccgcc agaagatgta
    30061 tgtgttcggg ctgggcgcgc cgggcggggc caacctggac ctggaagtga acgacgagag
    30121 cagcctctgg gcctactgga acggcggggg cagcacgggc atccgcatcg agagccgcgt
    30181 cacgaacctg gtcgaggatt tttccgggtt cgccgtcacc aacagcacgc tcgatctggg
    30241 ctccaacctc aatgcctacc tttccacgac gggctggacc gggtccaagg tgttttccga
    30301 cggggggagg gccaagctgg gctcgtccgc cgctcacggc agcctgacca ccccctcgct
    30361 ggacctggac gcggacctgg gccggtctcg gctgctcttc gacctggtcc gatggacctc
    30421 cgacacggcg gtcatccagg tcctgatgtc caccaacggc gcgccgttcc agctcgcggg
    30481 cggcgagatc accgcgcccg acatcatgac ccggcagacc ctggacctga tcgacggcgc
    30541 cgcggacacg cggatttgca ttcaggccaa gtggaccaac cagtgccgat tcaaactcga
    30601 caacgtccat atttccaagc gcgccgacac ctacgagttc acggacggcc tgtggcacca
    30661 cgtggcggtc tgccgcagcg gcgccgaggt ccgggtgtac tcggacggca tcctgcggga
    30721 aacagccgcg caggccggcg cgatcggcgc atccggcccg ctgcttctcg gctgcagttc
    30781 ctccacgtcc gccttctggt acggcgagat cgacgacgtg ggcctgtgga accgcgccct
    30841 ttcggaggcg gaagtcctcg cgttgtccga cgaccgcctg cccgaggcgc cctggcgttg
    30901 gacgcaggtc cagcacgcgg ccgccttttc atcccgcgac ggggccggcg acctggtgtt
    30961 cgacggccgc ctgtggctgc tcggcgggtg ggcgcccgag acggagcccg acccgtacac
    31021 cctgaacgaa gtgtgggttt ccagcaacgg cgcggactgg gcgtgggtga ccaacgcgcc
    31081 gtgggaaccc cggcataccg cgggctacgc cgtgcacgac gggaagatgt gggtcctcgg
    31141 cggcgacgcg aaccgcgggc actaccagaa cgacgtctgg tcctccacca acggagccga
    31201 ctgggtgcag gtcgcggcga ccgtgccctg gagcaaccgg gtcctgcatt acgcgaccga
    31261 gttcaacggc tgcctgtggg tcatgggcgg ccaggaactg ccccagctcg tgcccgggac
    31321 gccgaccaac gccgtattct acaacgacgt ctggcgctcc accaacggcg cggactgggt
    31381 ccgggtgctt gaccacgccc cgtggtcgcc ccgcggcatg atctcgggcc gggccgtatt
    31441 gagcaaccag ctctggctgg tcggcggggg cacctaccag acgcccaacg cgcccgaccg
    31501 gcagtatttc aacgacgtct ggagcacggc ggacggctcc aactggacct gcgcgacgcc
    31561 cgggagcccc tggcaggcgc gcctgtatca ccgcgtcggc gtctacgcca accgtctgtg
    31621 gatcaccggc ggcgcatacg atcagaacct caacgatgtc tggttctccg ccgacggggc
    31681 ctcatggctt caactggccg gaacgccctg gccgccccgg catgcccaca gcctttacct
    31741 gacggaggcg gcgctctgga tcacggctgg cagcagcaac gacctcttct gcatgcacga
    31801 cgtctggcgg ctggacagca cgaatgccgc cgcgtggccg gagccgccac cgctggccat
    31861 cgacggcccg gcaacgaacc ggatgtggct gggcttcgag ggcgagcagc accgcaccta
    31921 ctcgatcgaa ctcaacacca acctgctgtc gcgctgggag gcctggcagc tctccagcgc
    31981 ggagactacc cagcgcgtcg ttgaactcga cctggacgcg ttcgggccgc ggaacttttt
    32041 ccgcctggtc tggccatact acgtcctgcg gccggagtag gacgcgcttc tccttcgcgc
    32101 ttccgcccgc ccgccggata gcgtatggtg agcccgaatc atgaggctgg cggcgatata
    32161 caccttgtac ggccggcgag ccggcgcgga actcttcgtc gagcggaccc tggaggaact
    32221 cgggcggcag gtcccggact ggtccatcac ggtcttctgc aacgcccagg cccgcgacgc
    32281 gctccgggag cggctgcccg gcgtgaaggc cgagtacgtg ccgtggctgg accggcagtg
    32341 gaagaaggcg gtctggctcg aatggcgcgc gccgcgcgtc gtcacccgtc aggccttcga
    32401 tgtcttctgg gtgcccagcg gcagcgcgag ttttccgggg ccgtgggagg tcccgaccgt
    32461 ggggaccttc ctggacatgg gcggattcct ggtgaagaac cgcttccgcc gcagccgcac
    32521 gctatactgc cgatactggt ccacaccgcg cacgctgcgc cgggccgcgg ccctgaccag
    32581 tatctcgcag gccacggccg acgatctggt tcgcctgttc ccgggagccc gaaccccgcg
    32641 cgtcatccac ctggggccgt ccccgcgcgc gacgtccgcc cggctcgcag atccccggac
    32701 ggtgatccgc gaggaaacag gcctttcgct taaacacatc ctgttctcgc ccgcccgcac
    32761 ggactacagg ggcaagggcc gggacatcct gctccgcgcc tatgccgagt accggcggcg
    32821 cgcgccggcc cccctgccgc tggtcctccc cgggccgccg ggcgagtttc acgattccgt
    32881 gctgaaggac atccacgacc tgggcctcga gggatcggct gtctggccgg gccgcgtcag
    32941 cgacgcctgc atggaggcgc tctacgccat cagccgggcc ttgatcctgc catcgcgcac
    33001 ggagggattc gggttccccg tcctcgaggc catggaacga ggcgtaccgg tgatctgttc
    33061 cgacgcgggc tcgctgccgg aagtggccgg cgatgccgcc ctggtggtgc ccgcaggcaa
    33121 tgcggacgcg ctgctggaag ccctgctccg cctggagcgt gagccgggcc tgcgcgagga
    33181 actggcgcgc aggggacggg aacgctgccg cgccttctcg tgggagcaga cggggcgggc
    33241 ataccgggac gtcttcgaga ccgtcgtcag cggggccgct ggacatccac ccgataatac
    33301 cgccggtccg ccggcatgag cgggggcggc gaggaccgca gcaagtcctc ccgcatggtc
    33361 tgaaccggat ccccccggaa ggcggcggac tgcagcggcc accacgtccc cccggtcgag
    33421 gtccggcggg cgtacacccg gtacaggcgg ttcgtgctcg aggggaagga aatgctcacg
    33481 cggccgggat tcgtcgcgac caggctcacc cggaacacgc tgtcgcggtt catgggatgc
    33541 gtcatggaca cgtattcctt gtagttcgtc atcccgtccc cgtccgcgtc ggcatggggg
    33601 ttcagcgagg gcctgggttt cccgccgccc cggccctgcc atccgttgcc cgcctcgtcc
    33661 cagtattcca ggatatagcg gttgtattcc gccgtggacg ccgcgatctg gatgggcctg
    33721 acggtgatgt tgtcgaacca ggtcggccgc gaacgccacg tccacaggcc gaccgtaccc
    33781 gaggccagcg gcaaggcatc cacgattgtc cggccgaaca cgtccgcccc gttcacaaaa
    33841 acccgcactg tatccgccag cacctcgatc cggatgtgct gattgctgtt caccgtgtaa
    33901 ccgtgccagt tccaagccag catctcgttg gtcccgttcc gctgccgata taaccgtcgg
    33961 cacctgtttg tctggtccat ctcgaagcgg tagtagttgg aaggattctg gtatcggaag
    34021 accaagccga taccaccacc gtaccccgtc gtgatatccg cctccatggc gtagtcgctc
    34081 caggccgcgg cgacctccgc ggtccacagc aggaaggcgc cctgccggtt gaacagggag
    34141 ttccaggcag gcccgccgat gaaggaagcc tgcttgaccc ggttggattg cacggaccag
    34201 agcgacgggg cgttgagggt tccctcgtcc atcgcggccc agccggccag gtccccgtcc
    34261 gcgaagccct cctgcagcac cggggtggaa tgatcgaaca gccattcccc cgccgaccgg
    34321 tcgccgtccg gatgatgaga ttcccacccc ttgatgagca ggggatagca ggacccgtcc
    34381 tccatgggct cggtcgtcag gatcacctcg cgggcgcccg gggccagggt cacgccctcc
    34441 agggccaggg cctccccgaa actgtagttg gtgggagcca tcgcgtcgcg gctgtccacc
    34501 gccttgtcga aggacacacg cacctcgttc gatgccgcgg cccatacgtt gtcgaccgcg
    34561 ggcgcccgga cctcccgcgg cgtcaccacc accgccgaag ccgccccctc ggaccagcac
    34621 cccgcgccgt tgatggcgac cacccggtag gagcacagcg tgtccggcgg cgcggaccag
    34681 tcggtgcatt ccgcgccctg cacgaacgcc acctgctgcg tgtcccggaa gacagcgtag
    34741 cccagcaggc cgctctcccc gtccgcggcg ggctcccacg tcagccggca cgtcgaacca
    34801 tccaccgcct ccacccggag gcccgacggc gatccggggg gcgaggcatc gaacagcgcc
    34861 cggttggtgc aggcgaactg gttgttcgtg tcggttttat aattccccag gtgcggggag
    34921 tacgtggtga cattcaggcg gccggtgtcc ggcgtgaact gcaggatgcg aaggtaccct
    34981 gcgccgccat tggtcatcat ctggtaattg gccaccaccg cgtgcaccgt gttgctgaag
    35041 tctccctggt ccacgcgccg ggcgcctgtt tcccagggcg atgtcgccgc gtgcccattg
    35101 aacacgaacg cgatattgga gtgcttccga acgaacttat cccacatctc cagaccgttg
    35161 ttcacggggc catagccctc gtgcggatcg gaattgtgac tggccagcct tccctgccac
    35221 aggccgtcca ggtagagata ggagtgcgtc agcacgatcg cccggtggcg gggatagcgg
    35281 ttgagcagct ggtccgccca ggccagcgtg gcgtcgcccg gcccccactc cagggacacg
    35341 accagccagt ccaccccgcc cgccgtgaac acggcaccga tgttgtcaga ccgccccgcc
    35401 gggaaactgt ctacaacaga agctgtcgcc tggaaccggc ttagcgggaa gtactcgttg
    35461 aagcggtccg tgttcctcga gaagattccg gcgttgtaga agaaatcgtg attgccgtag
    35521 gtgaccaagt acggaacctt cccgtccagc ggcgccatgc actggctcgc gcgctgccac
    35581 tccagcgtgt tcgttccgcg atagctgtat gtgtgttcgg tgatgtcgcc ctcgtgcagg
    35641 acaaaacgga tgttcaggtt cgtccggttg gccagaatcc agtccgtttg agccgagaac
    35701 atcgccgaac gattggtcgt gctatcggtg tagtattgcg tatcgggcag gatgatgagg
    35761 gaaaaaggaa cttccgcccg cgcggcaagc acccccatga aaacgacaaa cgggtaaagc
    35821 gctttaccaa acagggcgtg caaatagagg tgctgacgaa tcatcgtgaa atctctgggg
    35881 agattaggag ggctcgggtg tcgtttattt cgatgactcg aaagccgttc tcttcacaga
    35941 aaaaaatagt gcgcaaaaca tactcgttcc caactcgttt tcaataaaaa aaacgccgcg
    36001 acattcgcgg cgatccggca tgcataatgg agggtttccg cccgaaagtc ccgatggggg
    36061 agaggatccc cgcacctagg ccatagcgtc caagagagcc ttcagttctt cccgtgtcag
    36121 ccggcgcgga ttgttcttta cgcggccggg attgaatccg tgatcgagta tcagcggaat
    36181 gtccgcgggc cggatgcccg cttcaccgag ccgaacgggc atccccacgt tccgcatcaa
    36241 atcggtgagc gccgccgcgg cgcccgccgg ggaatcggcg ccgataaggg ccatcagttc
    36301 cccgagaatg cgccccaccc atgccgcccc gcgcgcatcc tggcagtccg cgaccgtcac
    36361 gcccgcgttg aacgccagca tggacggcag cgtcagggcc gcggccaggc catgcgggac
    36421 gccgaaatac gacgtcagcg gatacgacac cgcgtggcag gccgttgtct tggagatgca
    36481 gatcgccttg cccgcgacgt gcgcggcctc ggccatgccg gcccgggcgg cggcgtcggg
    36541 cctgttcacg gcacggtcca ggtgctccag caccaggccc agcgcgcggc gcgcgtaacc
    36601 ctgcgattcg ggcgtagaga gcacggacca gaaggactcc gtcgcctggc acagagcgtc
    36661 cagcccggaa atggccgtct gccgcgggga gaggcgcgcc gtcaggccgg gatccacgat
    36721 agcgtagtcc ggcaggacga actcgtgctc gagcgactcc ttctcccggt ccacgtacag
    36781 caccgcaaag cgcgtcgcct cgctgccgga gccggccgtg gtcgggatcg ccgccagcgg
    36841 cgcgccgcgc cgccgcgggc cgcccgcatc ccgcaggcaa tcccgcgccc ccgcttcctg
    36901 ggcggccagg aagcgcaccg acttggccat gtccatcacg ctgccgccgc cggcggcgac
    36961 gacgaggtcg caggccgact cgcggaacag gcggattccg cgctcgacgt cctccagccg
    37021 cggattgctt tcgaagtcgt ggaaccggac gaacggcgtc gcgccgagga gcggctcgat
    37081 cgccgcccgc gcgccggagg cctcgtagga ggcccgcccg gtgaccagga acacccggcg
    37141 cggcgcgtgc cgttgcagca cctcgcccag gcaggccacc gagccggcgc cgagatattc
    37201 cgtttgcgcg ctcacgggat cacttcaggg cgcccagaaa cttcatcaac tcggtcttgt
    37261 tccgtaccgg cgtggtggtc gggcgcccga gatccttgcg cgcgccgggc cgcacccgca
    37321 ccgtcagcag gaacggcccc cggcccgcca gcatgctccg ggcggccgcc ttgagttcat
    37381 cctcggtttc ggccgcggcg ccgccggcat acccgaaggc ctcggccacc cggccgaaat
    37441 ccacggacgc ggacgcgagg cgctgccccc cggtcgaatc gtggaccccg ttgtcgaaga
    37501 ccacgtggta gaggttcgcc ggcgcggcgc ggccgatggc cgccaggttt ccggtatgca
    37561 tcagcatcgc cccgtcgccg tcgaggatga cgaccttcgc gcccggtttc tgcagggcca
    37621 cgcccagcgc gatcgcggaa gccagcccca tggacccgac ggtcaggaaa tccctctcgt
    37681 ggccctcccc gcgccgctcg cggatctcga acacctcgcg cgaggtcttg cccgtggtcg
    37741 ccacggagac ggccgccgcc ggcaggagtt ccaggagcca ttccagcgcc tgctcccgcg
    37801 tcagcgagcg gtcggaagcg ggggccaccg cgcggccttc cacgattccg ctacggacga
    37861 tcagggcgac cggggcgctg gcacgcaggg ccagggcgcg cagctcggcg atcaccgcgg
    37921 cggcgggatc cgccgccgcg ggcagatcgc gccacgggat ctccagggcg tcgagcagcg
    37981 gcctcatcac ccgccccatt ttgacgtgct gcggctcgtc cttgcgcccg ggctggccgc
    38041 gccagccgat catcaggatc atggggatgg cataaacctc gggatccgcc agcgaggtca
    38101 gcgggttgac cgtgttgccc aggccggcgt tctgcatgta gacgaccccg accttccccg
    38161 tcgccaggtg gtacccggcc gcgagggcca cggcctcgcc ctcgttcgag gccgcgaggt
    38221 ccgtcagccc ctgcccgttg ttcgcggaca ggaaagccat ccacggcgcg aacgtgctgt
    38281 ccggaacgcc ggtgaagaaa tcgagcccct cggcccggaa cgccgcccaa agttcttcgc
    38341 aggtcatcct tccgcccccg ggatcagcgt cagcacctgc ttgatgggca tgcagacctc
    38401 gtcggcctcg cggcagcggt gatggcgcag gatggattcc gcggccttcc gcatggccgg
    38461 gtaggcggcg cggagcaggt ggttggcgta gatcacgatc cggacgccgg cctccgcgag
    38521 ccggtcctcg ggcgtgtcgg cgtacgtggt gggcacgacg gccagcggcg cgggcgcgcc
    38581 catggcccga tagccctcgc agaagcgcag gatctcgtcc ggcgtcttct ccttgctgtg
    38641 aatgagcagg ccgtcggcgc ccgcctccag gtacgcccgg gcgcggcgca aggcgtcctc
    38701 catgcctttt ttcaggatca ggctctcgat gcgggcgatg atcatgaaat cgtccgtcgc
    38761 ctgcgcccgc ttgcccgccg tgatcttccc ggcgaacatc tccgcggatt cctgctcctg
    38821 ggccacgtcc gtgcctaaga gcgagttgcg cttcgggccg gtcttgtcct cgatcaccac
    38881 ggccgacacg ccctgcctct ccagggtgcg cacgaggtag acgaagtgct cggcgaagcc
    38941 gccgttgtcc gcgtccacga gcatgggctt ggtcgtgacg tccaggatct gctcgatggt
    39001 ctggaggcgc gaggtgaagt ccacgacgcc ggtgtcgggc ttgcccttcg aggcggagtc
    39061 ggtcaggctg ctttcccaca tcgcatcgaa cgagcgcgcg gccgtgccct cgccgaccga
    39121 gacctgctcc accagccgcg ccgtgagccc gttatggacc tccagcacgc ggaccagggg
    39181 cttgaggtcg agcagccgcc gcagcttgcg gcggcggacg tccggcgcgg agccgacctg
    39241 cagcacctcc cggatcagcg ccgtcgagct gacgtccggc gtgtattccg gctccacgag
    39301 ctcccctccc cactccttca gcgcggccat cacgcgcgcg cgggtctccc gctggacgcc
    39361 ctcgcgccag tcggtgccgt gcacgacgat gtcgggcttc agggcccgga gattcgggac
    39421 gtagtccaag gtctcctgcg gcaccacgcg cgcgacgccc tggatgttct cgatgatctg
    39481 cttgcgctgt tcgtacgaaa gcagcgggac acgcttgtag gacgcgatgg ccttgtcggc
    39541 cagcaggccg acggtcacct cgccgagctt ccgggcctcg cggatgatgt tgatgtgccc
    39601 gtgatgaatc aggtcggcgc acatcccgac ataaacttgt ttcatctcga ctcccttccc
    39661 ggccttcacg atgccttgtt cagccacttc cggttgacgc gcggcgaaca cgcgcacagc
    39721 cgcatcagcc attcctcggc cccgggcgcg tccgccggcg cggcgcgcca ggccccgccc
    39781 tcccgcgcca ggtattccag cgcgtccgtt tccgaatgat agagcacttt ccgcgattcc
    39841 gaaaccacgc cgtggtagtt gggcgcgtac ggcgacgggt gggctcccgc cagcggggcg
    39901 gtttcgacga acgcccaccg gtccccggcc tcctcgcgga aaaacgaccg gccgtccggc
    39961 tccagcgcgt cgcccgccgg ttcgaggccg caggcgtcca gcacggtcgg caggaggtcc
    40021 accgtgcgca gcagcccggc gcgacgggcg cccggcgcga atccggcgcc gaggaaaaag
    40081 gcgaaggtcc ggagggtgta gtcgttcagg taggtcccgt acatgcgctc gcccggccgc
    40141 tcgccgtagc tgcagccgtg gtcggaaaac acgaccagca agccgtcgcg gaagaaccgc
    40201 gcgcggtcca ggtcgtccag gagcgccgcc acgtagtcgc ccatctcgcg cgcgtacccg
    40261 cggtattccc gctcgttgcg ggcggggtcg gcgtagtaga gcgattcctt ctccccggga
    40321 tagacttcgc ggaacttctg gatcagctcg gtatgcgtct tgagattgtg cagcgagagc
    40381 aggtacggcc ggccgggcgc ccgcgcttcg gccagcagag cgcggtgccg ctcgaacagc
    40441 cggccgcggt ggtcgggatg aaactcgtcg tactccgtca cccgttcgta cccctggccg
    40501 ggaatcgtgt tccggttgtt gatgtccacg gcggtttcat agccggcggc gcggagatac
    40561 tgcgccaggg tgcggcaggc gtccgcacgg aacttcgcgg cggagaagta ggagtccacg
    40621 ccgttgcgcg ggccgtagac gcccgtgagg atcgcgtgca tggaggccgt ggtgtacggc
    40681 gcgtacgtga ccatgttccc gcagaaaagg ccgcgccgct tgaggccgtc aaacacgggg
    40741 tgcgcgtcca gcgcgtctgc gcgcaggccg tcgagcagga tcaacacgat ctgccgaggg
    40801 gcggtcattt gtatcccagt tggcggagca cttcgttcgc ctcgcgaagc tgctcgggcg
    40861 acaattgttc cgccgcggag ctcctgcgga tgtaccggct ggaaaagaag gccagttcgt
    40921 tcgccatcag ccgcgccacg cggggccgcg cggcgtagac gttcttctcc tcccccggat
    40981 cgttccgcac gtcgtacagt tccggctccg atccgagcag ctggcggtcg cgatcctcga
    41041 tccgccggta ggcgtccttc tcgcgcccgc cgaacaggac gtccagggcc cgccggaact
    41101 gctccttccg gcccttgcga ttgtgccaca aacgatagag caggcggcgg atgcgctgcc
    41161 acgtctcgcc gggaggacgc gagagcagga cagcccgctc ctccgggggc acgggggtga
    41221 tgagcttcca ccggcggttg cgcacagcga ggcgctggtt ctccggcgcg gccggcatga
    41281 aggtctcggc gtagatcaca gtgtcgtccg ccgcgtccgg attccgcgcc tcagccaggc
    41341 gcgaccggcc gttgaggccg gccggcgcgg cgcatccgag cgcctcgagg agcgtgggca
    41401 tcacgtcggt gttgggaaca agcgtggaaa cccgccgccc ggcgagaccc ggcaggccgt
    41461 gcagcaccag ggggacgcga agctcacggt cgtagagccg ccggccggac ccccacacgc
    41521 cgcgctcgcc caggtgcacc ccgtgatccg ccgtgaagac cgtcagcagg ttctccgccc
    41581 gcccgagatc gctcaacgcg gacaacaggc gggccacctg ccggtcggta tggttcagtt
    41641 ccccgccgta caggtaattc aggtgcgcga tgtcctccgg gccgggaacg aactcgccca
    41701 gcttgatagc taggccttcc ttttccgatc cgtccgcgcg cccggcgtag gcggtcggat
    41761 ggctttcgcg ccagtcgcgc gggggatcgt agggcgagtg cgtgtcgaac aggtgcagga
    41821 acaggaagaa cgggcggccg ggatcgaggc cgcgcagcca ttgcaacgcg aggcttacgg
    41881 ccgtcccggc atggcgcgag atcgtgcgct cgtccgggtc ggcgcccttg attcccttgg
    41941 gctcctcggg gccgaaggcg tcgtcgtaga aatcgaagcc gcggtccagg ccgcgcgacc
    42001 ggtccatcgt ggcgcagctg atcacggcgc cggtctggta gccttccgcc cggagccgct
    42061 ccgccagcgt cggcacgccg cgggcgagca cggaggtgtg cagccgcacg ccatggactt
    42121 tcgggaggca gccggtgaac agggtgctca cggacacgtt ggtcacgggc gagacggtga
    42181 aggcgttctc gaaaacgatc ccttccgccg ccagccggtc cacgaccggg gagttgccgc
    42241 gcgtcgatcc gtagcagccc agttgatccg gccgcagggc gtcgggcagc aggatcagca
    42301 gattccttct ttccagttca gccatgactc cgcctcttca cgctgccccg ccccggcgcg
    42361 cggccggtgg agaaaatagg acccggccgg gccgcgcgcc aatacaaatc gcaatccatc
    42421 gccggcttcc gccccccgtt cagcccggcc tcggcggctg ttccgggctc aaggtccaaa
    42481 ggcattcttc cggcatcccg ccggccaggc ggactcggag ccgctggact ttctcgcggc
    42541 ggccgtaccc gggggacatc tcgccgtcct ccacccggcc ttccgcctcg ggccgcgaac
    42601 aggcgagccg gaggacgccg gaaggcgcga ccaggcggaa gccggccgcc ccgtccctct
    42661 ccgcgagaac gcccggcgcc agcgtgaagt tccaggtcag cggttcgccc cccgccttcg
    42721 accatcggtc gtccaggatt acccggtccg cctccacctg gacgcgacgg ctacagcgga
    42781 aagttccgcc ggtcgtcgtt tcgacccgga tcccggagcg atcctgttcc gtgacgcggc
    42841 ccggtcgatc ggggccgggc aggcggaaca ggccgggcat gtccgtcgga aggggccacg
    42901 gctcccggcc gccgcgcgtc agcgcgttgt gcgccgccgc cgagcggaaa cgattgcgcg
    42961 cctccggatc cggcgtgtag aggtacgtcc cggggtcgac gaacaagcgg ctgccccgga
    43021 agttgatgac caccgagagc gtatcgttgt gcgcgtggct cccgtagccg catggcccgt
    43081 agccgccgaa ttccaggcag acctcgaagt c
//
LOCUS       DAOOTK010000017        41514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_708_length_41514_cov_5.407390, whole genome shotgun sequence.
ACCESSION   DAOOTK010000017 DAOOTK010000000
VERSION     DAOOTK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 41514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41514
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_708_length_41514_cov_5.407390"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..2687
                     /locus_tag="PK634_03615"
     CDS             459..2687
                     /locus_tag="PK634_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96853.1"
                     /translation="MKTRRRFQKRPYQPPRDKAVFSSVLREIGESVQGRNFAAGLSHA
                     NQLLATPALNRHEQARVLGMVGDSEFHRGAFEEAAVIYQRAATLSLDHDRLWLRPLVG
                     QVQALLKAVRVDEAQMMARHACDLARKKMADFNEQVSSANQLLASQGRVSVSLLPPRE
                     SVVATRMGSIFLREGEPALAREFFEKALLVNPGGACRARQGLARIALAMNDPARALTL
                     AEESIRGGRYAAKTLAAWPLLIAARRRLGGWQISQNLLNGLDMAPPGVRARAVLAITR
                     ELRNGDMRQWREVAQKWSTREGKRFPIVEAELRKMILASARAAPGDAEAKRKLAEELL
                     KTPGLSRNEWLAAAKECIRAGLWEGRRPNLDALIRHADAQYGGDSGPRTAHSLAISCM
                     MAKRHDLARPLLQRNIANLPAGKSLWGKSVWALARMESLLGNHMVAAGWYRTFCEADS
                     IPIRFRLQAQLLWMNALMAAGKPEALIEARPAIERLLAAVQDPEVLLDFARQLYSVSP
                     QLYSWSFQLFEQGERLALANFAAAQQPAVASEILFKLTRRQVNDFNRAGRAIDFWEKL
                     PAEKKEWLWSSQACFWEYLGWLAEAYGRQGGAGAAEVFARTWLDDPATPPEGAVCVGI
                     PYVKWLVGRCRADEALALCDRLVEEAPTHRSCAWAWYWKALKARKQGDAVEAKRCASA
                     IRRAQGVDRRLQPEWKLEARALLLLADLDPRRVDAQAVNYAPADWEAECSRLRLDMDR
                     LP"
     gene            2684..4072
                     /locus_tag="PK634_03620"
     CDS             2684..4072
                     /locus_tag="PK634_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96854.1"
                     /translation="MKKAILLHYLFFAVMSARAQVALTVGCPTPECPSLTVTVPTNAC
                     RPCTGSYVVVCASITNLAAGCYQNSNYNYSISQTNWKWKVDGIEAAPSSGIGECAEFT
                     NKTAGSGQITYSVDASMAVGPITCSVHETAIKPFTSHDLQLYLMGKTNLGINMYDPVC
                     SPTGSLTAKGPLVPEIAELPYTWTLEGVCIFDPVPDSNSTSAVIKEQCTPSPRYQHEV
                     VRVNKHCWTREQRFTVVQVDVSVGVSEGMEETQGVFIAVNDNDSDGSGYPDNTEAPLL
                     SDDPDLMPVTITICPADLPTNQMVSVSGIGHCYEDRRKMTNALSSYSVARVGQGLTLY
                     LEGASAGEVEFIDAVHPESGACDKAKYTVLKVEIVVELEDQEVNGVRSGLKQDTLKFD
                     FNDNVIHTSHLTLQPTTATVDGEQITTKLRVFYVPSCNELNLPGSNTVKVDIDDMVDN
                     HIDQVVNTFQLP"
     gene            4087..5553
                     /locus_tag="PK634_03625"
     CDS             4087..5553
                     /locus_tag="PK634_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96855.1"
                     /translation="MSNALLNDVIDRQWKVEPPAMTSALPSNKLGTLMGRATLRQCAA
                     LLLSCTLHCYAGENMKTPMQDGKSDPAVAIERARQGDPDELLRITQMAATHPQLLPER
                     KPLAQYHQYLQDTNVFVQYFAVQVLAGLKDKSSAEPLRQFIADTERRRKDLDSPGKKN
                     LDALGQKEAVALILASKTAFTALGEIDQGSPMSVKVLASQLNHDIPMEWGGGVAHGAL
                     AKMGHAGLRALLDEAAQPLDDNQKAFLKPAIASINDPDLAVDLYACCMDPKYHEVARK
                     SALWALGVMAKQSADIEQMVINIAEDETSDLRSMAIVRLGYIGSSRSRNTLLELEKTL
                     PQEAKAVQAALLACDATNRLPGMIETFLSPKTPLDEKRRVWGLLEGRSNELLPYARQL
                     LGVNDDGGVPINDLRVRVWIRLFELTKKRSSVELDYSDDERFMVVAGRIIICLTSELD
                     RRYAGTGKYTYAQLQQMALEETRRIVTKWGREEKEAKK"
     gene            5550..6860
                     /locus_tag="PK634_03630"
     CDS             5550..6860
                     /locus_tag="PK634_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96856.1"
                     /translation="MKRDWTCIIVVCLSSCMTAPRVVVAWGTALGSSLDTHHRIAKDV
                     VDNDIDATTYPDLVRFRDKLEDGSNTESHDLPDDNTTQWWGNGVGPAPETWFYADVKP
                     DGQEGTLSLYKKYSFEYAYKEMGFELHCAQDEFVPAHIRRCCHGTPGVYLLDDMEYWA
                     YLFTFGYASSSSPWTYSFTYSGGTSVFEYWLNDSMDDDDRDDIGSDADDEVDASGVLM
                     VDGPSAWGVPNTDWGTYGQPPYYLDEYGIPVLLEMLPGQNEGWDYYGDIADNSIMIRE
                     QLKKSYDGTLTRIKAHSAELPPLVPDDDTHGKPSISAKIFGPNKPVDVSLVAMENRQK
                     TVLVSMLAGAAPMKDTTGKVWDGGEAATYDLAADGNASSLPWKDMITRSWNGDLGTGQ
                     LADGNHSVKMKVKDQDGNDSEERTRSVKYDETKPTGTITVSVLP"
     gene            complement(6987..9074)
                     /locus_tag="PK634_03635"
     CDS             complement(6987..9074)
                     /locus_tag="PK634_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96857.1"
                     /translation="MLLPDPQFSVEANAWAFCAASLTARLLIPGWAWLWLWPPDGEPS
                     GPLARRLILTAQAALTGLLVSLVVVITLAAFGCYTETNEWMALAAATLTGLLAGEASG
                     RFRLAGTLRALLPGAAALLVAFAVLMGLPRRGEWVLGGWDPGIYVCQGVSLSQGGSFH
                     PDPDRFFAQLRVHELGLFTRPQHTYLEAHPVVPLDPARRSLEPFFFPGTPSLIAVLYR
                     CGGLRAATRVNDFAGLLATLAFGAAALALFRRGAPAWFSVLALLAQPAWLFHTHFPTS
                     EMLQLALLSGMALLLARRSLSLGAAVLFGILLFMAEVNRLSFLPFGMLLVFLLAANEL
                     EESTPRHTLARIIQVCALGGGTLFLFFTNLATIERLHQAIPPLFASSLVLVVLMLLFD
                     SGAFPRSARALVFRLQKMALGLGLGLVVALLAVAACAPGWLPDSLSWSLRGAARFMGA
                     GWIGLAALGALFILRTDSPMLHTWCLFLLGGWLASLLNPEIARLLPWAARRNLEYGVP
                     LVALLASGLPGFLWGRGGAPALRRITAVAILAVPLAAHGRAAATAWSATEHDGLTEVL
                     AGAAERTRPDDIIVADHFRWGTPLRFLFDRTVINGELYLDTRRFSRMQDALQVLARLA
                     AKGHRIRFLVSTERGLDVFPVVPSPTRLDWESSEWQDREVVQSPRARAVEVKQKPRRL
                     RLYTWELAAPITE"
     gene            complement(9085..9813)
                     /locus_tag="PK634_03640"
     CDS             complement(9085..9813)
                     /locus_tag="PK634_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOW96858.1"
                     /translation="MKRFADQGAVITGAGSGMGRSLAAGLAAEGARVCLVDRDEKSLA
                     ESVRLLGGPSPSVGSVLCDLTVDAEVSRMVQEAVRTLQRVDLLAHSAGAFRMGAIESL
                     PVAELDFQYRVNTRAPYALTQTLLPELRKSRGQVLFVNSSAGLAAGAKWGAYSATKAA
                     LKMLADSLRAEVNADGIRVLSIFPGRTAGPMQEQIHRLENRPYHADRLLQPDSVAAMA
                     LDALALPRTAEVTDVSIRPMMKSG"
     gene            complement(9815..11131)
                     /locus_tag="PK634_03645"
     CDS             complement(9815..11131)
                     /locus_tag="PK634_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOW96859.1"
                     /translation="MPTSIPPLQDPAAAGREMHALAADLFPICRSITGNGVRETLKRL
                     SALAPMSIREVPTGAPLLDWTAPKEWTIRDAWIKDPSGRKIVDFRASNLHVAGYSIPV
                     RRKIPLAELQQHLHSLPDHPDWIPYRTNYYGEDWGFCLAHRVRSALADGTYEVCIDST
                     LDAGSLTYGELVLPGAGPGEILVTAHVCHPSLADDNLSGLVVAAFLARHLKDTERRLT
                     LRFLFIPGTIGSIAWLALNETAVSRVRHGLVLACVGDAAPFTYKRSRRGDAPVDRAVA
                     HLLKHRPGGGRLVDFSPYGYDERQFNSPGFNLPVGCLMRSMYGTFPEYHTSADNLDFV
                     KPTSLAESAGLVLDVLDVLENNRRFLNLNPKGEPQLGRRGLYSWIGGQNDKAAAQMAL
                     LWVLNLSDGRHSLLDIAERADLPFPAIRNAAAQLQERGLLNEVEEA"
     gene            complement(11137..11667)
                     /gene="rfbC"
                     /locus_tag="PK634_03650"
     CDS             complement(11137..11667)
                     /gene="rfbC"
                     /locus_tag="PK634_03650"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOW96860.1"
                     /translation="MIFAPTAVAGVQRIAPERRGDDRGFFARTLCLKELRAHGIEFQP
                     VQCSTSFNARRGTLRGLHFQAEPRGEDKVIRCTRGRVFDVAVDLRPGSPTFRKAATEI
                     LSPDNGLQLFIPRGCAHGFLTLADDSEIEYMMAEYYFPDLARGVRWNDPAFAIRWPEE
                     PQVISDRDRTYPDFKG"
     gene            complement(11664..12905)
                     /locus_tag="PK634_03655"
     CDS             complement(11664..12905)
                     /locus_tag="PK634_03655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW96861.1"
                     /translation="MVHIALACRFCGHPLSQTFVDLGMSPLANSYLKADQLNRMEPFF
                     PLHVYVCENCFLVQLPEHESAEDIFSDYAYFASYSDSWLAHAKAYVQMISERLKLGPK
                     SLVVELASNDGYLLQYFVEKGVPVLGVEPAANVAKVAEQKGIRTLVKFFGEKLARELA
                     GDGTRADLIIGNNVLAHVPGLNDFVAGMKALLNQGGVITMEFPHLVQLMAHNQFDTIY
                     HEHFSYFSFTAVEKVFAKHGLTLFDVEELPTHGGSLRIYGRHADDASKPVGPRVLDLR
                     RREQAAGFETLEHYRSFGEQVKATKRALLAFLVKAKAEGKRIVGYGAPAKGNTLLNYC
                     GVRTDFLDYTVDRSPHKAGQFLPGSHIPIFPPEKIRETRPDYVLILPWNLKDEITQQM
                     AHIREWGGRFVVPIPTTQVLA"
     gene            complement(12985..14007)
                     /locus_tag="PK634_03660"
     CDS             complement(12985..14007)
                     /locus_tag="PK634_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW96862.1"
                     /translation="MKVLLTGILGYIGAFMGEALQEAHHRVTGLDTGYYARSVLYDKR
                     RAAPSVIHKDIRRVTEEDVTGYDAVLHLGELSNDPLGQLDPTLTHQINHAGSVGLAKL
                     CKKAGVPRFIYSSSCSVYGTGNGEFKDEQSPVSPQTAYAECKARVEREVAELADDRFS
                     PTFLRNATAFGLAPHFRFDLVLNNLSGLAATTGEIRMSSDGSPWRPLVHVRDICRAFV
                     CALEAPREAVHNQVFNVGDTKENYRVREVAEIVAQAFPGCKIATGNGDKDSRSYRVSF
                     DKIRQALPGFACAMTALDGARELKDIFDRIHLTRELFEHRAFIRLKQIRHLLATSQID
                     HQLFWN"
     gene            14165..14599
                     /locus_tag="PK634_03665"
     CDS             14165..14599
                     /locus_tag="PK634_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96863.1"
                     /translation="MTVTLPLRTLQQLQSISADRARAIVKAVDSATRADGSAGPKVEV
                     VEVLPGRSVILVGPSLYLKKIEFLHMAEITPTRFLLTLPSGTGLEALELAIADQLDDV
                     PDDESWEKELLSELLRILKSLRRSRGYFKAEMIFVKTPAARR"
     gene            complement(14607..15800)
                     /locus_tag="PK634_03670"
     CDS             complement(14607..15800)
                     /locus_tag="PK634_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOW96864.1"
                     /translation="MDTPAEPRVEPEKKARRGLLRALGRGIRAGWLYIGATILLLFVF
                     EAGINTLYHWDDFHNHPSRRFEAYRGADWVDAYVREELAVREGWAPFVHWRCEPREGR
                     DLRIGGDGLRRTWTRATPHDPLRRTFRVFVFGGSTTWGTGVRDEYTVPSWLARILSEE
                     YGLDVEVTNFGEIGYVSTQELILLLRQIQQGNLPDLAIFYDGVNDIFSAVQSRQAGLP
                     EHEFNRVREFNVLGRKRKALAALFWGSSQQGLPRFAATLARRLLHAERKPFALPDGLA
                     EDVVRTYAANVKLAEILARASGFPILFYWQPAIYSKASKPPEEQASENRLLFLKEFYL
                     DVYRRVREHPGLAADTAFHDISALLDDRPEACYIDYFHLSEEANGLIARRLAEDARAV
                     LQAPK"
     gene            complement(15820..16890)
                     /gene="rfbG"
                     /locus_tag="PK634_03675"
     CDS             complement(15820..16890)
                     /gene="rfbG"
                     /locus_tag="PK634_03675"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOW96865.1"
                     /translation="MNPNFWSGKKVLVTGHTGFKGSWLSLWLQKKGAQLTGFALPPPT
                     QPSLFELARVGDGMESVVADIATPGPILETLQRVRPEIVFHMAAQPLVQFSYQQPVET
                     YRTNVMGTVHLLEAVRQTPGVRAVVVVTSDKCYENKNWHWPYREFEPMGGRDPYASSK
                     GCAELVAAAYRTSFFGPESDGKYPAGVATVRAGNVIGGGDWTPGRLVPDVMKSLLAGQ
                     NVALRMVGAIRPWQHVLEPLGGYQLIAEQLWENPASCAEAWNFGPREESNQPVAWLVE
                     QVINAWGGEQSWKPDTEVYPHEDRFLKLDSSKAFNLLNWAPRLSLAETVRWIVEWYRV
                     LAAGGDIRQITEQQIRRFEELA"
     gene            complement(16887..17663)
                     /gene="rfbF"
                     /locus_tag="PK634_03680"
     CDS             complement(16887..17663)
                     /gene="rfbF"
                     /locus_tag="PK634_03680"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006277200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HOW96866.1"
                     /translation="MKTVILAGGFGTRLTEETALRPKPMVEIGEKPMLWHIMKIYSAH
                     GINDFIICCGYKAYVIKEYFANYFLHQSDVTLDLRNNKMEVHQHSVEPWKVTLIDTGD
                     KTLTGGRLKRVRPYVGDETFCMTYGDGLTDLDIRALIGFHKEQKTLATLTAVQPPGRF
                     GAFNLSEKQTKIPHFREKSQGDGAWINGGFFVLEPGVFDYIAGDSTTWEQEPLQKMAH
                     DGQLSAYRHGGFWQNMDTLRDKMLLEDLWASGKPPWKVWQ"
     gene            complement(17757..18404)
                     /locus_tag="PK634_03685"
     CDS             complement(17757..18404)
                     /locus_tag="PK634_03685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOW96867.1"
                     /translation="MNRFLGWLGAGFTAWGKLRAVCIRHYLLGRLARAGRGIALGEQV
                     RLYGARRIQVEDGVVIGHHVILRALESYPWTEPAQTFTPELALRRGCYIGHFTQISCV
                     QRVEIGEDVMIADRCFIADNQHTYLDPDRSVKAQPITVCGEVHIGAGSWIGANSSVAG
                     HVRIGRHCVVGANSVVTGDLPDFSVAVGAPARIIKRYDADRRAWRATGPDGAWAS"
     gene            complement(18574..19980)
                     /locus_tag="PK634_03690"
     CDS             complement(18574..19980)
                     /locus_tag="PK634_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOW96868.1"
                     /translation="MNQFLAESEVEAGRIPSRWAHLVNIFFASFGFVALRFILSPIRL
                     KILAELLSKEQYGTLTLINNTVTFVMMVSSLGSLEFLLRKIPGRPDDYQNGMFKTILL
                     YFGGLSVALALAGMGWLAWLPMGKIRLSGSDILACGLLLVLTVHLNQYFHYFLGLSKY
                     ALSRIAQLLQGDTWFIPLIPFFWWGRLQVGHILWIWVIWLALTAALMQRWIRFDRVWP
                     ARASRAELAEVLRFGVPLVPLILGERLFMVQDRYVLVYFMDTEAVANYALCTNIAMVG
                     LMVGLAVLDILLIEFFKLRNRVEGCSIHELANHPELRHHLTNMLRYALLIGLPFGAAL
                     VYLGDPIIRVLSSAKFLDAAPILVWLAPLPLFSFLFVIVGRVLLGFNENARVGAASLA
                     AAIINIELNLILVPRLDERGAALAAVTSYALLVLYLGGRLRVWRWLIWDDLKPVRLLL
                     LLLISLLGCRWSANYLAG"
     gene            complement(20046..20798)
                     /locus_tag="PK634_03695"
     CDS             complement(20046..20798)
                     /locus_tag="PK634_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96869.1"
                     /translation="MKRPSRIGLAAGALLLRAALSHGQMLAEVSSRTADGWQVGAGFE
                     NFTANYTIDTVYKNLTTPDFSWDLFSLHGALALGPRLDVFLVAALIDEARNSDHPDIS
                     GEGGTFGAGLRGLLWEKNGFDLSAYGQLSYATEDFSDERLWDEHIGSHTNERSLEFTE
                     LILGLVASYTRGGFTLYAGPETLAYEDGNYHYTTLLEDGSVFAEETLDIEQTDKTSLR
                     IGARYRFESLWLAVSAALVHEEAELFSAGWVF"
     gene            21017..21574
                     /locus_tag="PK634_03700"
     CDS             21017..21574
                     /locus_tag="PK634_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96870.1"
                     /translation="MLKRFLLIFLLLAGVGLLAGVTGCEDSNDGGDDVVDVEPTDNSD
                     DSADDDADQADDDSDQSNDDAADQQDDVTPTPEPEPEPEPVSVNLTGDWQRPGLGSIY
                     RLNQRGVSLQGAYFELEDPYVSGDIAGTVVGSIIEMDVAVHYTDGIRTDFVAHKSGSI
                     ISSDHIRLVVTSSPVAQGQVQNWYR"
     gene            complement(21582..22586)
                     /locus_tag="PK634_03705"
     CDS             complement(21582..22586)
                     /locus_tag="PK634_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOW96871.1"
                     /translation="MEKSTGTSVTPPKPRKRFRWLGILLMMGVSLVVALALGEWVCRL
                     AHLGDIILYERGMYVESTDPDLAYELKPGYDGTSYGSEVAVNSMGFRGPECSTEPRPG
                     VFRVVCVGDSFTFGMGVKYEQAWPARLQTELPPPAGFREVEVINAGVSGYNLIQYCQE
                     IADKVIPLKPHLVVVGLVGNDLAPSFHAERGYLVVPRKKTTLPIPGKRWLQTHSHLYQ
                     FINMKYQSAWTATLEKKNPQLAHELTWQDRDQKAWAEAQLRLTRMRRELRDRGIQIVV
                     ARLLLPENSPLPDIVHHADVPIVPVEIARQDRLADGHPNAAGHESIAQQLAAVLPNFL
                     "
     gene            complement(22559..23590)
                     /locus_tag="PK634_03710"
     CDS             complement(22559..23590)
                     /locus_tag="PK634_03710"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96872.1"
                     /translation="MRTAQLASAAVSAALWAVAAWAQDSASFRALCAEKAAAAEAAGA
                     TAVEGLGGWLFLPAELRHLGAGRFWGEDAAAVSRATDPASADPLPAIVDFQQQLAKAG
                     VELIFVPVPPKAVIYPEGVSDAPPAGRADEFHREFYEQLRQAGVCVLDLTDDFLAAKA
                     AGPALYCKQDTHWSGQACIVAARKLAGEIFSRPWATDLPRRDWAPETRDVEITGDLWS
                     SLNRPDLPREKLPLRFVRDAAGAPPADDRASPLVFLGDSHNLIFHAGADMQAAGAGLA
                     DQLALELGTAVDVLGVRGSGATPSRINFYRRIKADPAYLSGKKLVIWCLSAREFTEST
                     GWKKVPVLP"
     gene            complement(23590..25305)
                     /locus_tag="PK634_03715"
     CDS             complement(23590..25305)
                     /locus_tag="PK634_03715"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96873.1"
                     /translation="MSAPKQTLSREDIAKIEIGHTDISRGLVRLLFFAFLATIGAVPA
                     WQHAREIGEHRAGRRADALPQCYDTFKCAVRAFRQWPERPGPFFRRVAALNALLLRDI
                     HAYEDELEQSSRLGQAVLPRAQYALTRWLGVGNEKTYVGRDGWLFYRPEIDYLAGPGF
                     LDPKQLRKRAAGGSEWEAAPHPDPVASIARFRDQLAKRGIKLVVMPAPVKPMVHPERF
                     SRAYEGREEALQNPSYADFITRLRMSGAHVFDAADELARARRETGEPQFLATDTHWRP
                     ESMERTAEALAAYLRDIAGLPDAAPAGYRRDAQAITNHGDIALMLKLPAGSDLFPPEA
                     ATIHPVLTAGGLLWQADAASDVLFLGDSFCNIYSLDSMGWGGNAGFVEQLSFCLQRPV
                     DRIVRNDAGAHATRQMLAQELARGRDRLAGKRVVVWEFAARELAVGDWKPVELSLGQP
                     PPATFLVPAPGTVETLSGTVAAIAPVPKPGSVPYKDHIVAIHLVDIARPSGPVPGGEA
                     LVYAWSMRDNVWEPAARLRAGQSIRLQVKNWADVAVQYEAINRTELDDDRLMLEDPCW
                     GEVTE"
     gene            complement(25307..26767)
                     /locus_tag="PK634_03720"
     CDS             complement(25307..26767)
                     /locus_tag="PK634_03720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOW96874.1"
                     /translation="MVFSSHLFIFYFLPAALALYYLMPRRGKHLLLTIASYVFYGWAN
                     PYFVLLMLYSTLIDYVCGLQLARQFRREAWSQPVPLLERNSPRSRGQKAAVVISIVQN
                     LALLGFFKYFNFATESYDALAGWLGFPNLRLDLAFKITLPLGISFYTFQSMSYSIDVY
                     RGDARAIRNFTDFACYVAMFPQLVAGPIIRFSEVADQMMQRTHTLEKFARGIAFFTLG
                     LAKKVLLANPCGKVADTVFNAGSAGCADSWFGVLSYSFQIYFDFSGYSDMAIGLGLMF
                     GFVFAKNFDSPYHSQSITEFWRRWHLSLSTWLRDYLYIPLGGNKHGPVRTYVNLFLVM
                     FLGGLWHGAAWNFVAWGAMHGILLAFERFWNKRPVYHFLPRPLRVGLTYVLVLFTWVF
                     FRAKDLPAAVAYLKSMLGLGTAQDGAGLLQGILYKPYYLLTFALCAVIVWFAPQAWDW
                     SRKITGRKALAVIVLLWLSIVVLSTQAYNPFIYFIF"
     gene            complement(26768..28147)
                     /locus_tag="PK634_03725"
     CDS             complement(26768..28147)
                     /locus_tag="PK634_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96875.1"
                     /translation="MKHHTVAGLFLAAAWLAGAAQADDLDARVRAFTGARTRAVWCRQ
                     AEGAGEDVFARGRQLQVWGYDSGDGLGERVILGAVTNYHRPMITPKGDRIVFNNLRDR
                     LISVVRWDGSGLRTLTNGTAADVWLDPATGVEWVYMMVREIEEFHSGPVIRFQLDNPA
                     VSEVVLDRQDVTWDNFQLSADGTRASGQFPHPRAGYVRLPGGDETVLGKGCWTSMAPD
                     NSYLSWIFDGPHRNLHLFAPDGRHWKVTINGAPGVAGYEVYHPRWSNHARYFAMTGPY
                     KVGPQGKNLIAAGGGAVDVYLGRFSPDFTAVEDWLKLTGADQADFFPDLWIEGGRGAA
                     VTLPAGQPAQAPEAATKPGTRVKVEARLAASIPTPSVASISPYTQALVVYTYEVVKVL
                     EGECSAPRILVAHWAIRDKQVLDLDKEQGGTYPLELELFDAHPELEGERLFMETEEFD
                     LPLFYSTGN"
     gene            complement(28212..29213)
                     /locus_tag="PK634_03730"
     CDS             complement(28212..29213)
                     /locus_tag="PK634_03730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW96876.1"
                     /translation="MGPRHAAWFLLAGFLAATAGRAETAAVDFPAEPAAARRQVAGSP
                     YAADSAGMRLEFVTDATRLVLTVSYADQADMADHRYFLSEGEWIVDGEPRLSFTRASG
                     GGGAQDVELALDGPPRVRKHEIYLPLADRVEFRGLAVNEGARVRRQPPVEGRRVVAYG
                     DSITQGFYATRPSRSYPAQLARATGWNVMNMGFCGRITTPEDAAAIASLKPDAVLVLI
                     GENDVLLGTDLAVFAERYQAFLEELIRRCPGLPIVAATPPEARGRKWNTGLIEDYRQA
                     IRVAAAERPAVHLVEGPELLAGSRKLLADGLHPNDAGFETLARSFENALRPAFDAHP"
     gene            29354..29854
                     /locus_tag="PK634_03735"
     CDS             29354..29854
                     /locus_tag="PK634_03735"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOW96877.1"
                     /translation="MASLIGMSAEVKGKSFPIDRDEITIGRAKDNVVAIESPTVSGHH
                     CAIVRDGARYLLKDLESTNGTRLNSKDVSEAKLRPKDIVQIGSVEFLFDAEQTEAVDT
                     HSYAEAQIEVAPGPTAAPESFNSISPFGARRTESSGVGLLVITLVGLLALVIVGLFVV
                     KLIFSD"
     gene            29861..30520
                     /locus_tag="PK634_03740"
     CDS             29861..30520
                     /locus_tag="PK634_03740"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOW96878.1"
                     /translation="MRLPRRRRPAGFTLLELLVAMLIAGLIVGVVYTLYHTVTLAVSG
                     RRVRDDQVARVGCAFQSVCADLSSLFPGPSDDGLIRLGFAEAGQTNFSDLVFCAVRAP
                     GSEPDMRWAQAVQVRYRVDTPSAGGGELLCETRALAGPGAEAVETNRLLAGVAGFRVS
                     LFDGETWKAEWGGTAERIAPRAARIELETDGGKELVRTEVSIAAGLVVSGRLERAAAG
                     P"
     gene            complement(30541..30780)
                     /locus_tag="PK634_03745"
     CDS             complement(30541..30780)
                     /locus_tag="PK634_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96879.1"
                     /translation="MAGKQAKAHMLGVGLDHRDEHVRITRGPNFTVLMGSEETHESLR
                     GACMKINEKLRRRGKRIETVSAEEFFDMVREAGGS"
     gene            30779..31150
                     /locus_tag="PK634_03750"
     CDS             30779..31150
                     /locus_tag="PK634_03750"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HOW96880.1"
                     /translation="MACASSIVAMQNILGGRARGKGRVPGLLEDAYRRYHRPECIRPD
                     PLEVALRYADPRDQEIAGLVAALLAYGGVKAIMRSATAVLASLGPSPSRTLRQADPRR
                     WRSRRRFAGCARRTPCAGISR"
     gene            31319..34168
                     /locus_tag="PK634_03755"
     CDS             31319..34168
                     /locus_tag="PK634_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96881.1"
                     /translation="MTKLCARRSRAVWVPSSLLLLTLLVPGVTAQELNVDQEFEFARA
                     LIPLGFADYSDKVVQNVLRLHPDMKDRATVIQAEILVSKRKFAEAEEMVKAMDPASPK
                     AQAISLAIGKGYYLIGEQDKARGIFEAFFKQYEGRLPTDADLLTFYREAAFQFGQMLE
                     MAGDLAGAVAAYDKYLQTKPDPNVVRALMAKQAEAHVKLAESLTGAEQETHLVAAEKL
                     STELQWKGLDIWFGQSLITVAHAHLIRGKPADAQKFLTQNMDILKEIDTYIRDNDMSM
                     SMSPMAGARFLLGELYQKDAEALAGGGKKDEAVQAYGKAITEFINVFAQYSDSDWGPK
                     AGLRAQEIKKILVEQYGKQVKWNMDEKAVAQVSEASFRLADNLYLQKKYPDAIGEYTK
                     ILKQYPEGEPSQKALANLLDCYIQGNDALMIKATAEYIGERFGGKDIPAMALLAAGKT
                     YFDRKDEAMYTYVYETYLKHFPKHNRAAAILFTLAGLRKGAGDEAGAAKYYERIVENY
                     PKDQYYPKALSAMAWGYFLATNYEGAVKGFQLFLPAAQPNPDKVQAQFALAECFRMLG
                     RWTEALPEYEKLIGWLVPKDNPYATSAADAKKNADILEKAVFQRAFAYSRMGGSPDEV
                     KANREKGIRAYDQFTASFPNSTLASKALDAKGRMQLEIGQFDAATKTFDDLAAKFPTT
                     DEGKNALFSLIRSAVEVKQFEQALAAFEKMYGQRDKFSPDEFARVGQLMLDGGQYAQA
                     VEAFQYVTVKVPEAALDPKEERALLERALFGLGRAAFEQGQFEAASKAMDDLMTRYPK
                     SGTFYEAKFIQARANKELGKLPEAIAALGDIMKYADDTVLRNRTTYELALMQKQKGDK
                     ADALASYLRVALLADPKNPALTELVEKSLMESIDLGMEMQRYQDVLDSCEQYMQAFPQ
                     GSRIEDARRLRNEARMKAAAAAARPASTNAPAGGK"
     gene            34197..34991
                     /locus_tag="PK634_03760"
     CDS             34197..34991
                     /locus_tag="PK634_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96882.1"
                     /translation="MNTYRSKSAAWKLAGLLLTVAVAAQAAYVILSDNRRMEGTEIRM
                     TADGSVVLTTDRGPVTLSKGQFKLAMADKVPAEWEKVGQAVQAKKYDDAITQLNELAR
                     KYRGLGLDVQAIQMLSKVQMMKGDPGAAVTAIETLFRMNPAAKGSPELAWSFREALLG
                     AKQYDRLNTELNEVVASGSRPDAARAQIMRGDIKMAQGQGEGAVMDYLRTVVFFGSEQ
                     AVQPEALFKAADTLEKLRDPRAKEMFKKLVQEYPSSPEAQQARSRI"
     gene            35041..35880
                     /locus_tag="PK634_03765"
     CDS             35041..35880
                     /locus_tag="PK634_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOW96883.1"
                     /translation="MSSKYLVWAMLAVSLVAWGVTVWAQEPAAAPAAEPAAAPAGAGA
                     DNALALSEEAKAAEEAEANKLDTGFWAMIISSGGLGITLWLSLLATSIAAAGLTIDSF
                     INIREKKIVPQALVTSVRESMEQGDVMKALKICEDAPGSLANILSAGFGNVKEGFDVI
                     QDVVGVAADLEGEKLLQRVAYLNVCANLAPMLGLLGTVQGMIYAFASLATMTAGAAQQ
                     AALALNISQALWTTAAGLVIAIPAISFYTFFRNRATKIMLGMEGLTMDLIKSLRNVEV
                     VEE"
     gene            35929..36354
                     /locus_tag="PK634_03770"
     CDS             35929..36354
                     /locus_tag="PK634_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOW96884.1"
                     /translation="MRKRSEEPGINMTPMIDVVFQLIIFFVTTVDLESKAIDENLKMA
                     MAPHGVAVEKKDPRTVFVDVDSSGAISIARVRMSAPMLRLVLRKSVAEYGQTVPVVIQ
                     ADGRTKHEHVKAVMDSCAAAGLWKVKFAAKKEEAQKPKS"
     gene            36371..36781
                     /locus_tag="PK634_03775"
     CDS             36371..36781
                     /locus_tag="PK634_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOW96885.1"
                     /translation="MARKRRKSEMPAADLPMTPMIDVVFQLLIYFLVTIKPVDVIAHL
                     DVFRPSPEASQEKPDTPPKMIKIVIFPDGFVINDKPVDLPDLDRLLSRLAAIDTKQTI
                     LIMVSAASPHEKLIQVLDLCSKSKLVNLSVVSTN"
     gene            36803..38284
                     /locus_tag="PK634_03780"
     CDS             36803..38284
                     /locus_tag="PK634_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOW96886.1"
                     /translation="MKKLDAKAIARWIKAHIWGPTGSVIIHILIVLALLKLVTGTSSN
                     KAPEIEVMMMEPDAVDLEEFEQELEKLEELPEMVDAVTPPEVSLTEQPPDVQASDFAA
                     PQPNVDFAALDVQQDFQSPLVMQGLFAGRSSGGRASSLREYGGQWGQYTEAAVLRALE
                     WLKNRQNPDGSWKGRLDPDTVGYTGLAILTFLAHGETTASEKYGATVEKGIRYLMGRQ
                     EENGAFFKKFDGSGPVYAQAIATYALSEAYGLTRIPALKPVMDKAVKVLLDGQQAKGG
                     WDYSYAKGARRDTSVTGWHVQALKAAYIAGAETPGIHEAMEKALMDLRSVQDPETGTF
                     GYTSQTDRKSPKSLGMTGVAVLCMQLMGYSSDSAARKGSDVLSSATCDWNDPMNSPFY
                     AWYYITQSRFHRGGAGWSEWNNKVAPTLVRNQDPKGFWPDSKADNVVGERGGAESDPQ
                     TIYRTTLAALTLQVYYRLLPTYKAVAIEPSTPATKEDIAVEIL"
     gene            38394..40163
                     /gene="mutL"
                     /locus_tag="PK634_03785"
     CDS             38394..40163
                     /gene="mutL"
                     /locus_tag="PK634_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOW96887.1"
                     /translation="MPGPAHIRLLSDEVINKIAAGEVVDRPASVLKELLENALDAGAT
                     RVTVEIVAGGRKMIAVSDDGGGMDRDDALLSIERHATSKIRDVDDIEDVATLGFRGEA
                     LAAIAAVSQFTLTTRTAAAEGGTEITVAGGKIRDVRDEGCPAGTRVQVRNLFFNVPAR
                     RKFLRSEQTELSHVRQVFLLYALSHPAVGLALRVDEREVWRLAAGATLNDRLLELFGP
                     GTISQLRPVDFRAGELWLHGYAGIPSLSRGDRSDQYFFINGRPAGAPLLHFALSEAYH
                     TLLPRGRYPVLYLFLELPPGQVDVNVHPAKKEVRFRNTGAVRDAVIEGLRRALGTTPA
                     APEAAPIHPLASVAPPAEAWLPAIRRDQPPAFAYPRLPMAPAEGEAPAPGPSGSPAAA
                     PAAGPWAWCRVLGQVGGLYVVMETPDGVVLMDPHAAHERVLFERFMKEVTAHRVNSQG
                     LLAPETVALLPQDARRVREQLDLLRDMGFGLSEFGADTFLVDALPVCLGNVSAAALLA
                     EVADTLERAGARGGTERWAEEAVAQASCKAAVKARDQLAPEEIEELVRALAATGMPYT
                     CPHGRPTVIFMSFGELHRKFGRA"
     gene            complement(40164..40721)
                     /locus_tag="PK634_03790"
     CDS             complement(40164..40721)
                     /locus_tag="PK634_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96888.1"
                     /translation="MKKNHFDINRPVHLARRDVFFERAAELLYTPFQRLPTAQRIQHI
                     ETVLSLLKTSEQHAFFAIQASRALPREHDMLRFLRLIHDSVQSAHAMMQHQLHLEGEE
                     SFLCQFLSATPEQCVLPAMHYQRRAEDILQGLWNVLQLAHTAYRQLQQANQDQFLPEE
                     RERYRLAYESFREDVMQLPVASALA"
     gene            40962..>41514
                     /locus_tag="PK634_03795"
     CDS             40962..>41514
                     /locus_tag="PK634_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOW96889.1"
                     /translation="MRRVQVRRWTVLALGLAGLAQTGFGAGFALYEGSARGNALGGAM
                     AGSADDASALYCNPAGITQLEGVQVMGGMTLIAPSTKVTTMTPAGSVTTETEENLWIP
                     PHLYATWEASDRVWLGFGMYSRFGLGTEFDENWPGRYNSYYARIRSIELNPNIAYKVN
                     DKFSIAAGLNATWFELKLEQAISP"
BASE COUNT         7328 a        13351 c        14092 g         6743 t
ORIGIN      
        1 catgaagcct gtatcacgga ggaggttcat acaggtgacc ggcgccgcga cagtggggct
       61 ggccttgcat tccatggtgg tcgatcgcgt ctttcggccg cgcgcggcac gcacccctgt
      121 ttggctccga ctcgtatctt ctgcgggtgt cagatcgtcc gatgaagctt tttgcaggcg
      181 cgcccgtttc cgcacggcgg cggatgctat gcgggtcgcg gcacgaagag gaatctccgt
      241 ggagatttgc tgtgaaggat agtgttttcg atttcgccct cgggctgcgc gtaacaacgc
      301 tacgtcgcaa ttcgagggcc ttggccggga accgaaaaaa ccctgcgagg attccaggtt
      361 cccggccgcc tttttatggg taattggtaa cgcgggcttc tcgcctgtgg tcgttgaagc
      421 ggcggctctg tgagccgcct agggaattaa ggcgggacat gaaaacaagg cggcgattcc
      481 agaagcgtcc gtatcaaccg ccccgagaca aggctgtttt ctcttcggtc ttgcgagaaa
      541 tcggtgaaag tgttcaaggg cgaaatttcg cggctggtct gtctcacgcc aatcaactcc
      601 tcgcgacccc agcgttgaac cggcatgaac aggctcgcgt cctgggtatg gtcggcgaca
      661 gcgaattcca tcgcggcgcc tttgaagagg cggcggtcat ttaccaacgg gcggccacgc
      721 ttagcctgga ccatgaccgt ttgtggctgc gtccgctcgt gggccaggtt caagccctgc
      781 tcaaggccgt tcgcgtggac gaggcgcaga tgatggcgcg tcatgcctgc gacctggccc
      841 ggaagaagat ggccgacttc aatgagcaag tgagttcggc caaccagctc ctggcttcgc
      901 agggtcgcgt ttcggtttcc cttctgccgc cgcgcgagag cgttgtcgcg acaaggatgg
      961 ggtccatctt ccttcgggaa ggcgagccgg cgctggctag ggaattcttt gagaaggccc
     1021 tgctggtgaa tccgggcggc gcctgccgtg cgcgacaggg gctggcgcgt atcgcactgg
     1081 ccatgaacga tccagcccgg gcgttgaccc tggcggaaga atccatccgc gggggccgct
     1141 acgcggccaa gacacttgcg gcttggcccc tcctgatcgc ggcccggcgg aggctggggg
     1201 gctggcagat cagccaaaac ctgttgaacg gcctggatat ggcccccccc ggcgtgcgcg
     1261 cgcgcgccgt cctggccatc acgcgagaac ttcggaacgg cgacatgcgc cagtggcgag
     1321 aggtcgcgca gaagtggtcc acccgtgagg gaaagaggtt cccgattgtt gaggcggaac
     1381 tccgcaagat gatcctggcg tcagccaggg ctgcgcccgg tgacgcggaa gcaaaaagga
     1441 aactggcgga agaacttctc aagacgccgg gcttgagccg caatgaatgg ctggcggcgg
     1501 cgaaggaatg catccgcgcc ggtttgtggg aaggacgccg tccgaatctg gacgctctta
     1561 ttcgacatgc tgatgcgcag tatggtggcg attccggccc gcgcaccgcg cacagcctgg
     1621 cgatctcctg catgatggcc aaacgccacg atctggcccg gccgttgctg caacgcaata
     1681 tagccaatct tccggccggg aagagcctgt ggggcaaatc ggtctgggcc ttggcccgca
     1741 tggaaagcct gttggggaat catatggttg cggcgggatg gtaccgaacg ttctgcgaag
     1801 ccgattccat tccgatccgt tttcgcttgc aggcccagtt gctctggatg aacgccctca
     1861 tggccgcagg caagcccgag gccttgatcg aggctcgtcc ggccatcgag cgcctgttgg
     1921 ccgcggttca ggatcctgaa gtgctgttgg attttgcccg tcagctgtac tctgtatctc
     1981 ctcaattgta ctcgtggagc tttcagttgt ttgagcaggg tgaacggctg gcccttgcga
     2041 acttcgcggc ggctcaacag ccggcggtgg cctcggagat ccttttcaag ctgacccgtc
     2101 gacaggtaaa cgatttcaat cgtgcaggcc gcgcgattga tttctgggag aaactgcccg
     2161 cagagaagaa ggaatggctt tggtcgtccc aggcctgttt ctgggaatac ctgggctggc
     2221 tggcggaggc atacggtcgg cagggcgggg ccggcgcggc ggaggtattt gctcgaacgt
     2281 ggctggatga tccggccacg cccccggaag gggcggtgtg cgtgggtatt ccgtatgtca
     2341 aatggctggt aggccgctgt cgcgcggacg aggctttagc cctgtgcgat cggcttgtcg
     2401 aagaggctcc tacccaccgc tcgtgcgcct gggcctggta ttggaaagca ctgaaggccc
     2461 gcaagcaggg tgatgctgtt gaagcgaagc gctgcgcttc cgcgatacgc cgcgctcaag
     2521 gcgtggacag gcgtttgcag ccggaatgga aactggaggc gcgggcgctg ctgctgttgg
     2581 ccgatctcga tccgcgtcgt gttgatgcgc aggctgttaa ttacgcgccc gcggactggg
     2641 aagcggaatg ctcgcgactc cgccttgata tggaccgact gccatgaaaa aggcgatcct
     2701 cctccattac cttttttttg cggtgatgtc cgcccgggcc caggtggcgc tcacggtggg
     2761 atgcccgacg ccggaatgcc cgtccttgac ggtgacggtt ccgacgaatg cttgccggcc
     2821 ctgcacaggc tcctatgttg tcgtgtgcgc cagtataacc aatctagccg cgggatgtta
     2881 tcagaacagc aactacaact actcgatatc ccaaaccaat tggaaatgga aggtggatgg
     2941 tatcgaagcg gctccaagca gcggcatagg cgaatgcgcc gagttcacca acaaaaccgc
     3001 gggaagtggt caaatcactt acagtgtgga tgcctcgatg gccgtcggcc cgattacctg
     3061 ttccgtgcac gaaaccgcga taaagccatt tacctcgcac gatcttcaac tttacctgat
     3121 gggtaaaacc aatctgggca tcaatatgta cgatcctgtt tgcagcccta ctggttcgct
     3181 taccgccaag gggccgcttg tccccgagat cgcggagctg ccctacacat ggacccttga
     3241 gggggtgtgt atcttcgacc cagtgcccga ttccaattca acaagcgcag tcatcaagga
     3301 gcaatgcacg cccagtccca gatatcagca tgaagtcgtg cgggttaata agcactgctg
     3361 gacccgcgag caacggttca cggtggtcca ggtggatgtt tctgtaggcg tgtccgaagg
     3421 catggaagag acccagggtg ttttcatcgc cgtgaacgac aacgacagcg acggaagcgg
     3481 gtacccggac aacacggaag cgccgctcct ttcggacgat ccggacctga tgcccgtcac
     3541 catcaccatc tgtcctgcgg accttcccac aaaccagatg gtttccgtgt cagggattgg
     3601 gcattgctat gaagaccggc gtaaaatgac caacgccctc tcgtcctatt ccgtagcccg
     3661 cgtcggccag ggcctgacgt tgtacctgga gggcgcctcg gcgggcgagg tggaattcat
     3721 cgacgccgtt catccggaaa gcggcgcctg cgataaagcg aagtataccg tcctgaaagt
     3781 ggagatcgtt gttgagcttg aagatcagga agtgaatggc gtcaggagcg gcttgaagca
     3841 ggatacactc aagttcgact tcaacgataa tgtgatccat acttcacatc ttacgcttca
     3901 acccacgaca gcaacggtcg atggcgaaca gatcacaaca aagttgaggg tgttttatgt
     3961 gccgtcatgt aacgaactca acttgccggg ttccaacacc gtcaaggtgg acatcgacga
     4021 catggttgac aaccacattg atcaggtggt caatacgttt caactcccat aaggaactca
     4081 atcgccatga gcaacgcact attgaacgat gtgattgatc gtcaatggaa ggtcgagccc
     4141 cctgctatga catccgctct gccgtccaac aaactcggca cccttatggg cagagccacg
     4201 ctacgtcagt gcgcggcact gctgctctca tgcacgctgc actgctatgc gggagagaac
     4261 atgaagaccc cgatgcagga cggcaaatcc gatcccgcgg tggcgatcga gcgtgcacgg
     4321 caaggcgatc cggatgaatt gcttcgcatc acccagatgg cggcaacgca tccgcagttg
     4381 cttccggagc gcaagccgct ggctcagtat caccagtacc ttcaggatac gaatgtgttc
     4441 gtccagtact ttgctgttca agtgctcgcc gggttgaagg acaaatcaag cgccgaaccg
     4501 ttgaggcagt tcattgcgga cactgaacgc cgacggaaag accttgattc ccccggcaag
     4561 aagaaccttg acgcactagg tcagaaggaa gccgttgcgc tgattttagc cagtaagact
     4621 gcttttacgg cgcttggcga gattgaccaa ggttcaccca tgagcgtgaa ggtcctggca
     4681 tcccagttga accacgacat acccatggag tggggtggtg gcgttgcgca tggcgcgctg
     4741 gccaagatgg ggcacgctgg tttgcgcgca ttgctggacg aggctgccca acccttggac
     4801 gacaaccaga aggccttcct caaacccgcg atcgcgagta tcaatgatcc tgatcttgct
     4861 gtggatctgt acgcctgctg catggacccc aagtatcatg aagtggcccg caagtccgcg
     4921 ctgtgggctc ttggggtgat ggccaaacag tctgccgaca ttgaacagat ggtgattaac
     4981 attgccgaag acgaaacatc agatcttcgg tcaatggcga tagtgcgtct tggatacatc
     5041 ggttcttccc ggtcccgtaa cacgctgctc gagttggaaa agacgctgcc ccaggaagcg
     5101 aaagccgtgc aagccgcact tctggcatgc gacgccacaa accgtctgcc agggatgatt
     5161 gagacgtttc tttctcccaa gacaccgctc gacgagaaaa ggcgggtgtg ggggttattg
     5221 gaagggagaa gcaacgaact ccttccttat gcaaggcagt tgctaggagt caacgacgac
     5281 ggcggtgttc cgatcaatga cttgcgagtt cgcgtatgga ttaggctttt cgagttgacg
     5341 aagaaacgct catcggttga actcgattat tctgacgacg aacgattcat ggtcgttgcc
     5401 gggcgtatca tcatttgcct tacaagcgaa ttggatcgac gatacgccgg gacggggaag
     5461 tatacctacg ctcaactcca acaaatggca ttggaagaaa cgaggagaat agtcaccaaa
     5521 tggggccgag aagagaagga ggcgaagaaa tgaaacgcga ctggacatgc atcatcgtgg
     5581 tttgcctgtc ttcatgcatg acggctcctc gcgtggttgt ggcttggggc accgcgctcg
     5641 gatcaagcct ggatacccat catcggatcg cgaaggatgt cgtggacaat gacatcgacg
     5701 cgacaacgta tcctgacctt gtccgattca gggacaaact ggaagacgga tcgaataccg
     5761 agtcgcatga tttaccggac gacaacacca cccagtggtg gggcaacggt gtcgggcccg
     5821 cccctgaaac gtggttctat gcggacgtga aaccggatgg ccaagagggc accctgtctc
     5881 tgtacaagaa gtacagtttt gaatatgcat acaaagaaat gggtttcgag ctccattgtg
     5941 ctcaagatga gtttgtgcct gctcacatcc gtcggtgttg tcatggcact ccgggtgtgt
     6001 acttgctgga cgacatggaa tactgggcat acttgttcac atttggatat gcgtcgagtt
     6061 ccagtccgtg gacgtactcc ttcacgtatt ccggcggcac ctcggtgttc gaatactggc
     6121 tcaacgattc gatggacgac gatgacagag acgatatagg aagcgatgca gatgatgagg
     6181 ttgatgccag tggcgtgctg atggttgacg gcccctccgc gtggggagtg ccaaacacgg
     6241 attggggaac ctacggccaa ccgccttact acttggacga gtacggcatt cctgtgttac
     6301 ttgaaatgct tcccggtcaa aatgaagggt gggactacta tggagatatc gccgacaatt
     6361 cgattatgat ccgcgaacag ttgaagaagt cctatgatgg aactctcaca cggattaagg
     6421 cgcactctgc tgaactgcca ccgctggtgc cggacgatga cacacatggc aaaccttcta
     6481 tctcggcgaa gatctttggc ccgaataagc ccgttgatgt gtctcttgtg gccatggaga
     6541 accgccagaa gacggttttg gtctcgatgc tcgccggcgc cgctcccatg aaggacacga
     6601 cgggtaaagt ctgggatggc ggcgaagccg ccacatacga cttggcggcc gacggcaatg
     6661 cgtcttcgct tccatggaag gacatgataa cacgttcatg gaacggcgat cttggaactg
     6721 ggcaactggc ggacgggaac catagcgtca agatgaaggt caaagaccaa gatggtaatg
     6781 actcagagga acgaacgcgt tcggtgaaat acgatgagac gaagccaacg ggaacgataa
     6841 cggtaagcgt tctgccgtaa ggtggcggcg gctcaactca taccttgtag tgaccttcat
     6901 gaatagaggc gcccatatgt tcgtagtgca tcatcggggt cagccctcaa atagcgaaga
     6961 gcggcttccg aagtgctgcc cggagttcat tccgtgatcg gggcggcgag ttcccaggtg
     7021 tagagccgca agcggcgggg tttctgtttc acttccacgg cgcgcgcgcg cgggctctgg
     7081 acgacttcgc ggtcctgcca ctccgacgat tcccagtcga gccgggttgg ggaggggacg
     7141 accggaaaga cgtcgaggcc gcgctcggtc gagaccagga accggatgcg atgtcccttc
     7201 gcggccaggc gcgccagcac ctgcagggcg tcctgcatcc ggctgaaccg gcgcgtgtcc
     7261 aggtataatt ccccgttgat cacggtgcgg tcgaaaagga agcgcagcgg ggtgccccag
     7321 cggaaatgat cggccacgat gatgtcgtcg ggccgggtcc gttccgccgc gccggccagg
     7381 acctccgtca gcccgtcgtg ctccgtggcg gaccaggccg tcgcggccgc ccggccgtgc
     7441 gccgccagcg ggacggctag gatcgcgacg gccgtgatac ggcggagcgc cggcgcgccg
     7501 ccacggcccc acaggaagcc gggcaggccg gacgccagca gcgccaccag gggcactcca
     7561 tattccaggt tccggcgggc cgcccagggc agcagccggg cgatctccgg gttcagcagg
     7621 ctggccagcc agccgcccag caggaacagg caccaggtat ggagcatcgg gctgtccgtc
     7681 cgcaggatga agagggcccc cagggcggcc aagccgatcc agccggcgcc catgaaccgc
     7741 gcggcgcccc ggagcgacca cgagagcgaa tccggcagcc agcccggcgc gcaggcggcg
     7801 accgccagca gcgcgacgac caggcccagg cccaggccca acgccatttt ctgcagccgg
     7861 aagacaaggg cccgggcgga acgcgggaac gcgccggaat cgaaaagcag catcagcacg
     7921 accaggacca ggctggacgc gaagaggggc gggatcgcct ggtgcagccg ctcgatcgtc
     7981 gccaggttgg tgaagaacag gaacagcgtg ccgccgccca gcgcgcagac ctggatgatg
     8041 cgggccagcg tgtgccgagg ggtggattcc tcgagctcat tggcggccag gagaaacacg
     8101 aggagcatgc cgaagggcag gaaggatagc cggttgacct ccgccatgaa cagcagaatg
     8161 ccgaacagga ccgccgctcc gagggacagg gaacgccgcg ctagtagcag cgccatgccg
     8221 gagaggagcg cgagttgcag catctccgag gtgggaaaat gcgtgtggaa cagccaggcc
     8281 ggctgggcga gcagggccag gaccgagaac caggccgggg cgccccggcg gaacagggcg
     8341 agcgccgccg cgccgaacgc gagcgtcgcg agcagacccg cgaaatcatt gacccgggtg
     8401 gccgcccgca gtccgccaca gcgatacagc acggcgatca gcgatggggt ccccgggaag
     8461 aagaatggct ccagcgaccg ccgggcgggg tccagcggaa ccaccgggtg cgcttccagg
     8521 tacgtgtgct gcgggcgggt gaataggccg agttcatgca cgcggagctg cgcgaaaaag
     8581 cggtccgggt ccggatggaa cgagccgccc tggctcaagc tcacgccctg gcacacgtaa
     8641 atgcccgggt cccacccgcc cagcacccat tcgccgcgcc gcggtaaccc catgagcacg
     8701 gcgaaggcga ccagcagcgc ggcggccccc gggagcaggg cgcggagggt tcccgccagg
     8761 cggaaccgtc cgctggcctc gcccgcgagc aggccggtaa gcgtggccgc ggcgagcgcc
     8821 atccattcgt tcgtctcggt atagcagccg aacgccgcca gcgtgatgac aacgaccagc
     8881 gagacgagca ggcccgtcaa tgcggcctgc gcggtcagga tcagtcgccg ggccaatggc
     8941 ccgctcggct ctccgtccgg cggccagagc cagagccacg cccagcccgg gatcaggagg
     9001 cgcgcggtca acgacgccgc gcagaaggcc catgcattgg cctcgacgct gaattgcggg
     9061 tccgggagga gcatgggcag ggcctcaacc ggacttcatc atggggcgga tggaaacgtc
     9121 cgtcacttcg gccgtccttg gcagggccag ggcgtccagc gccatggcgg ccaccgaatc
     9181 cggctggagc agccggtccg cgtggtaggg ccggttctcc aggcggtgga tctgttcctg
     9241 catcgggccg gccgtgcggc cggggaagat gctcaacacg cggatgccgt cggcgttgac
     9301 ctccgcgcgc aggctgtcgg ccagcatttt aagggccgcc ttggtcgccg agtaggcgcc
     9361 ccacttcgcg ccggcggcca ggcccgccga ggagttgacg aacaacacct gcccgcggct
     9421 cttccgcagt tccggcagca gggtctgggt cagcgcgtac ggggcgcgcg tgttgacgcg
     9481 gtactggaaa tcgagttcgg cgacgggcag ggattcgatg gcgcccatcc ggaacgcgcc
     9541 ggcgctgtgg gcgaggaggt ccacccgctg gagcgtccgg acagcttcct ggaccatgcg
     9601 tgacacctcc gcgtcgacgg tgaggtcgca cagcacggag ccgacggagg gagacgggcc
     9661 gcccaggagg cgcaccgatt ccgccaggct tttctcgtcg cggtccacga ggcagacccg
     9721 ggcgccctcg gccgccaggc ccgcggccag gctgcgcccc atcccgctcc ccgcgccggt
     9781 gatcacggcg ccttggtcgg cgaatcgttt catgtcatgc ctcctcgacc tcgttcagga
     9841 ggccgcgctc ctgcagttgg gccgccgcgt tgcggatcgc cgggaagggc aggtccgccc
     9901 gctcggcgat atccagcagc gaatgccggc cgtccgagag attcagcacc cacagcagcg
     9961 ccatctgggc cgccgccttg tcgttctgcc cgccgatcca ggagtagagc ccgcgccggc
    10021 cgagctgggg ctcgcctttc gggttcaggt tcaggaatcg ccggttgttt tccaggacgt
    10081 cgagaacgtc cagcacgagg cctgccgatt cggcgagcga tgtcggcttc acgaaatcca
    10141 ggttgtccgc cgacgtgtgg tattccggga acgtgccgta catcgagcgc atcaggcagc
    10201 cgacgggaag gttgaagccg ggcgaattga actgccgctc gtcgtagccg tacgggctga
    10261 aatccaccag ccggccgccg ccggggcggt gtttcaggag gtgggccacg gcccgatcca
    10321 ccggcgcgtc gccgcggcgg ctgcgcttgt acgtgaaggg cgcggcatcg cccacgcagg
    10381 cgaggaccag tccgtgccgg acgcgcgaga ccgccgtctc gttgagggcg agccacgcga
    10441 tcgaaccgat cgtgccgggg atgaacagaa aacggagggt gagccggcgc tctgtgtcct
    10501 tcaggtgccg ggccaggaag gccgccacca ccaggccgct caagttgtcg tcggcgagcg
    10561 acggatggca gacgtgcgcg gtgaccagga tttccccggg gccggcgccc ggcaggacca
    10621 gctcgccgta cgtcagcgag ccggcatcga gcgtggaatc gatgcagacc tcgtacgttc
    10681 catcggccag cgcggaacga acacggtggg ccaggcagaa gccccagtcc tcgccgtagt
    10741 agttcgtccg gtacgggatc cagtccgggt gatcgggcag ggagtgcagg tgctgctgca
    10801 gttcggcgag cggaatcttt ctgcggacgg ggatgctgta acccgcgacg tgcaggttgg
    10861 aggcccggaa atccacgatc ttccggcccg acgggtcctt gatccaggcg tcgcggatgg
    10921 tccattcctt cggggcggtc cagtcgagca ggggcgcgcc ggtcggcacc tcgcggatcg
    10981 acatcggcgc cagggcggag aggcgcttca gcgtctcgcg gacgccgttt cccgtgatgc
    11041 tgcggcagat cgggaacagg tccgcggcca gggcatgcat ttcccggccg gccgctgcgg
    11101 gatcctggag agggggaatc gaggtcggca tggtcgtcat cccttgaagt ccggatacgt
    11161 ccggtcgcgg tccgaaatca cctgcggttc ctccggccac cggatggcga aggccgggtc
    11221 gttccaccgg acgccgcgcg cgagatccgg aaagtagtac tcggccatca tgtactcgat
    11281 ttccgagtcg tcggcgaggg tcaggaaccc gtgggcgcag ccccgcggga tgaagagttg
    11341 caggccgttg tctggcgaga ggatctccgt cgccgccttc cggaacgtgg gcgagccggg
    11401 ccggaggtcc acggcgacgt cgaagacccg gccccgggtg cagcggatga ccttgtcctc
    11461 gccgcgcggc tcggcctgga agtgcaggcc gcgcagcgtg ccccgccggg cgttgaacga
    11521 ggtgctgcac tggacgggct ggaactcgat gccgtgcgcg cggagttcct tcaggcagag
    11581 cgtgcgcgcg aagaacccgc ggtcatcccc ccggcgctcc ggcgcgatgc gctgcacacc
    11641 ggccacggcg gtcggggcga agatcatgcg agcacctgcg tcgtcgggat gggcacgacg
    11701 aaccggccgc cccactcgcg gatgtgggcc atctgctgcg tgatctcgtc cttcaggttc
    11761 caggggagga tcaggacgta gtccggccgc gtctcgcgga ttttttcggg cgggaagatc
    11821 gggatgtggc tgcccggcag gaactggccg gccttgtgcg ggctgcggtc caccgtgtag
    11881 tcgaggaaat cagtccgcac gccgcagtag ttgagcagcg tgttgccctt cgccggcgcg
    11941 ccgtagccga cgatgcgctt gccctcggcc ttggccttca ccaggaacgc cagcagcgcg
    12001 cgcttggtgg ccttcacctg ctcgccgaac gaccggtagt gctccagggt ctcgaagccg
    12061 gcggcctgct cgcggcggcg caggtcgagg acgcgcgggc cgaccggctt ggaggcgtcg
    12121 tccgcgtgcc gcccgtagat ccgcagcgag ccgccgtgcg tcggcagttc ctcgacgtcg
    12181 aacagcgtca ggccgtgctt cgcgaacacc ttctcgaccg ccgtgaagga gaagtaggag
    12241 aagtgctcgt ggtagatcgt gtcgaactgg ttgtgggcca tcagctgcac caggtgcggg
    12301 aattccatgg tgatgacgcc gccctggttt agcagcgcct tcatgcccgc cacgaaatcg
    12361 ttcaggccgg gcacgtgggc cagcacgttg ttgccgatga tcaggtccgc gcgcgtgccg
    12421 tcgcccgcca gttcccgcgc gagcttttcg ccgaagaact tcaccagggt ccggatgccc
    12481 ttctgctccg cgaccttcgc gacgttggcc gcgggctcga cgcccagcac cggcacgcct
    12541 ttctccacga agtactgcag caggtagccg tcgttgctcg ccagctcgac gacgaggctc
    12601 ttcgggccga gtttcagccg ctcgctgatc atctggacgt aggccttcgc gtgggccagc
    12661 cagctgtcgg agtaggaggc gaagtaggcg tagtcgctga agatgtcctc cgcggactcg
    12721 tgctcgggca gctgcacgag gaagcagttc tcgcagacgt acacgtgcag cgggaagaac
    12781 ggctccatgc ggttgagctg gtcggccttc aggtacgagt tggccagcgg cgacatgccg
    12841 aggtccacga acgtctggga taggggatgg ccgcagaagc ggcaggctag cgcgatgtgc
    12901 accataggat gttgctttcc atgggtttgg atgacgctac acgaatcctt tcgtcagcgg
    12961 accgagaata gggcagggac gccctcaatt ccagaacaat tgatggtcga tctggctcgt
    13021 cgccagcagg tgccggatct gtttcaggcg gatgaaggcg cggtgctcga acagctcgcg
    13081 cgtgagatgg atccggtcga agatgtcctt cagctcccgc gccccgtcga gggcggtcat
    13141 cgcgcaggcg aagcccggca gcgcctgccg gatcttgtcg aacgagaccc ggtagctgcg
    13201 gctgtccttg tccccgttgc ccgtggcgat cttgcagccc gggaaggcct gcgcgacgat
    13261 ctcggcgacc tcgcggaccc ggtagttctc cttcgtgtcg cccacgttga acacctggtt
    13321 gtgcaccgcc tcgcgcggcg cctccagcgc gcatacgaac gcccggcaga tgtcccggac
    13381 gtggaccagc gggcgccacg gcgagccgtc gctgctcatc cggatctcgc cggtggtggc
    13441 ggccaggccg gacaggttgt tcaagaccag gtcgaaccgg aagtgcgggg cgaggccgaa
    13501 cgccgttgcg ttgcgcagga aggtggggga aaagcggtcg tccgcgagct cggcgacctc
    13561 gcgctccacg cgggccttgc actcggcgta ggcggtctgg gggctgaccg gcgactgctc
    13621 gtccttgaac tcgccgttgc cggtcccgta cacgctgcac gaggacgaat agatgaacct
    13681 cggcacgccg gccttcttac agagcttggc gaggccgacg ctcccggcat ggttaatctg
    13741 gtgggtgagg gtcgggtcca actggccgag cggatcgttg gacagttcgc cgagatgcag
    13801 gacggcatcg tagccggtga cgtcctcctc cgtgacccgg cggatgtcct tgtggatgac
    13861 cgagggcgcc gcgcgccgct tgtcatatag aacagaccgg gcatagtatc cggtgtccag
    13921 gcccgtgacc cggtgatggg cctcctgaag cgcctcgccc atgaatgcgc cgatgtaccc
    13981 gaggataccc gtcagcaaga ctttcattgg actcccgctc cccgaaaaaa cctgttgaca
    14041 cccggatagt ttctacgtac aaactgcgtc tacaaatcaa tattccaata gcatgggcgt
    14101 ttctgcaacc aataaaaaag gggcgggggg gcggcctcgt aaattcaatg aatccagccg
    14161 tcctgtgacg gttacgctgc ccctgcgcac gctccagcaa ctgcaatcca tctcggcgga
    14221 ccgggcccgg gccatcgtga aggccgtgga ttcggcaacg agggcggacg gctcggccgg
    14281 gccgaaggtg gaggtcgtgg aggtgcttcc gggccgctcg gtgatcctgg tcgggcccag
    14341 cctttacctg aagaagattg agtttctgca catggccgag atcaccccga cccgcttcct
    14401 gctgaccctc ccgtcgggga cgggcctcga ggcgctcgag ttggccatcg cggaccagtt
    14461 ggacgatgtt cccgacgacg aatcctggga gaaggaactc ctgtcggaac tgctccgcat
    14521 tttgaaaagc ctgcgccgga gccgggggta tttcaaggcg gagatgatct tcgtgaagac
    14581 gcccgccgcg cgccgctgac cggcggctac ttcggggcct gcaggacggc ccgggcatcc
    14641 tcggccagcc gcctcgcgat cagaccgttg gcctcttcgc tcaagtggaa gtaatcgatg
    14701 tagcacgcct ccggccggtc atcgagcagg gccgagatgt cgtggaacgc cgtgtccgcg
    14761 gccagccccg ggtgctcccg cacgcggcgg tagacgtcga ggtaaaactc cttcaggaac
    14821 agcagccggt tctcgctggc ctgctcctcc ggcggcttgg aggccttgga gtagatcgcc
    14881 ggctgccagt agaacaggat gggaaacccg ctggcccgcg cgaggatctc ggccagtttc
    14941 acgttggccg cataggtgcg aaccacgtct tccgcgagcc cgtccggaag ggcgaacggt
    15001 tttcgctcgg catgcaggag cctccgggcc agagtcgcgg cgaagcgggg caggccctgc
    15061 tggctggagc cccagaagag cgcggccagc gccttgcgtt tccggccgag gacgttgaac
    15121 tcgcggaccc ggttgaactc gtgctcgggc aggccggcct gccggctctg cacggccgaa
    15181 aagatgtcgt tcaccccgtc gtagaagatg gccaggtcgg ggaggttccc ctgctggatc
    15241 tggcgcagga gcaggatcag ctcctgggtc gagacgtagc cgatctcccc gaaattggtg
    15301 acctcgacgt ccagcccgta ttcctccgac aggatccggg cgagccagga ggggaccgtg
    15361 tactcgtcgc ggacgccggt gccccaggtg gtcgagccgc cgaacacgaa gacgcggaag
    15421 gtgcggcgca aggggtcgtg gggcgtcgcg cgtgtccagg tgcgccgcag cccgtccccg
    15481 ccgatccgca ggtcccggcc ttctctcggc tcgcagcgcc aatgcacgaa cggtgcccag
    15541 ccctcgcgca cggccagttc ctcccgcacg taggcgtcca cccagtccgc gccccggtag
    15601 gcctcgaagc gccggctggg atggttgtgg aaatcgtccc agtgatagag ggtgttgatg
    15661 cccgcctcga agacgaacag gaggagaatc gtggcgccga tgtacagcca gcccgcgcga
    15721 atcccgcggc ccagggcgcg gagaaggccc cggcgcgcct tcttttcggg ttcgacgcgg
    15781 ggctcggcgg gggtgtccat ggccggaggg ccgcccggtt caggccaatt cctcgaaccg
    15841 gcggatctgc tgctcggtga tctgccggat atcgccgccc gccgcgagga cgcggtacca
    15901 ctccacgatc caccggaccg tctcggccag gctcaagcgc ggcgcccagt tcagcaggtt
    15961 gaaggccttg gacgagtcca gcttcaggaa ccggtcttcg tgcggataga cttcggtgtc
    16021 cggcttccag gactgctctc cgccccatgc gttgatcacc tgctccacga gccaagccac
    16081 gggctggttg ctctcctcgc gtggcccgaa gttccaggcc tcggcgcagg aggccgggtt
    16141 ctcccacagc tgctccgcga tcagctggta gccgccgagc ggctccagca cgtgctgcca
    16201 cgggcggatc gcgccgacca tgcgtagggc gacgttctgc ccggcgagca gcgatttcat
    16261 gacgtcgggc accaggcggc ccggggtcca gtcgccgccg ccgatcacgt tgcccgcgcg
    16321 gactgtggcg acgccggccg ggtacttgcc gtccgactcc gggccgaaga aggaggtccg
    16381 gtaggccgcc gccaccagtt cggcgcagcc cttgctgctg gcgtaggggt cgcggccgcc
    16441 catcggctcg aactcgcggt agggccagtg ccagttcttg ttctcgtagc acttgtcgct
    16501 ggtcacgacc accacggcgc gcacaccggg cgtctggcgc acggcctcga gcaggtggac
    16561 cgtgcccatg acgttggtcc ggtaggtttc caccggctgc tggtaggaga actgcaccag
    16621 cggctgcgcg gccatgtgga agacgatctc cggccggacc cgctgcaggg tttccaggat
    16681 ggggcccggc gtcgcgatgt cggcgaccac ggactccatg ccgtcgccga cgcgggccag
    16741 ctcgaacagg ctgggctggg tcggcggggg cagggcgaac ccggtgagct gcgcgccctt
    16801 cttctggagc cacagggaca gccagctgcc cttgaacccc gtgtgcccgg tgaccaggac
    16861 cttcttgccg ctccagaaat tcggattcat tgccacacct tccagggcgg cttaccggag
    16921 gcccacaggt cctcgaggag catcttgtcc cgcagcgtgt ccatgttctg ccagaatccc
    16981 ccgtgccggt aggccgagag ctggccgtcg tgcgccatct tctgcagcgg ttcctgctcc
    17041 cacgtggtcg agtcgccggc gatatagtcg aagaccccgg gctccagcac gaagaacccg
    17101 ccgttgatcc acgcgccgtc cccctgcgac ttttccctga aatgcgggat cttcgtctgc
    17161 ttctccgaga ggttgaacgc gccgaaccgg ccgggcggct ggacggcggt cagggtggcc
    17221 agcgtcttct gttccttgtg aaaaccgatc aacgcgcgaa tgtcgaggtc ggtcaggccg
    17281 tcgccgtagg tcatgcagaa cgtctcgtcc ccgacgtacg gccggacgcg tttcagccgg
    17341 ccgccggtca gcgtcttgtc gccggtgtcg atcagcgtca ccttccacgg ctcgacggaa
    17401 tgctggtgga cttccatctt gttgttccgg aggtccagcg tgacgtccga ctggtgcagg
    17461 aagtagttgg cgaagtactc cttgatgacg taagccttgt agccgcagca gataatgaag
    17521 tcattgatcc cgtgggcgga gtagatcttc atgatgtgcc agagcatcgg cttctcgccg
    17581 atctccacca tgggcttcgg gcgcagcgcg gtctcctccg tcagccgcgt gccgaacccg
    17641 ccggccagta tgacagtctt catgaatcgt ccccgatcgt gacggccctg caaacaatgg
    17701 gctttgttcc taccccgtgg cgcggcgatt ggcaagccta attgccggcc gcgccgtcac
    17761 gaagcccacg cgccgtccgg cccggtcgcg cgccaggcgc gccggtcggc gtcgtatcgc
    17821 ttgatgatgc gcgccggggc gccgacggcc acgctgaaat ccggcaggtc gcccgtgacc
    17881 acggagttgg cgccgaccac gcagtgccgc ccgatgcgca cgtggccggc cacgctggaa
    17941 ttcgcgccga tccacgagcc cgcgccgatg tggacctccc cgcacacggt gatcggctgg
    18001 gccttcacgc tccggtcggg atcgaggtac gtgtgctggt tgtccgcgat gaagcagcgg
    18061 tcggcgatca tgacgtcctc cccgatctcc acccgctgga cgcaggaaat ctgcgtgaag
    18121 tggccgatgt agcagccccg ccggagcgcc agctcggggg tgaacgtctg cgcgggctcc
    18181 gtccatggat agctttccag ggcccgcagg atcacgtggt ggccgatgac gaccccgtcc
    18241 tccacctgga tgcgccgggc cccgtacagc cggacctgtt cccccagcgc gatcccgcgg
    18301 cccgcgcggg caagccgccc gagaaggtag tggcggatgc acacggcccg cagcttgccc
    18361 caggccgtga atcccgcgcc caaccagccg agaaacctgt tcatatccca tcccttccgt
    18421 tctgccgcgc ggcagcccgt ctcaattctt tccgccgcct tttttggcga acgtgccgag
    18481 caccaggtgc aggtccgatt tctgcatcag gcccagcagg accatcgcca gcgccatcca
    18541 caccagcgcc gccgccagca ccgcgaggct gccctagccc gccaggtagt tcgcgctcca
    18601 ccggcagccg aggaggctga tcagcagcag gaggagcagc cgcacgggtt tcaggtcgtc
    18661 ccagatcagc cagcgccaga cccgcagccg cccgcccagg tacaggacca ggagcgcata
    18721 actggtgacc gccgccaggg ccgcgccccg ttcgtccagc cgcggcacca ggatcaggtt
    18781 cagctcgatg ttgatgatcg ccgcggccag ggaggccgcg ccgacccggg cgttttcgtt
    18841 gaagccgagc agcacgcggc cgacgatgac gaacaggaag gaaaacaggg gcagcggggc
    18901 cagccagacc aggatgggcg ccgcgtcgag gaacttggcg ctgctcaaca cgcggatgat
    18961 ggggtccccg aggtacacca gcgcggcgcc gaaggggagc ccgatgagca gcgcgtaccg
    19021 cagcatgttg gtcaggtgat gcctgagttc gggatggttg gccagctcgt ggatcgagca
    19081 gccctcgacc cggttccgca gcttaaagaa ctcgatcagg aggatgtcca gcacggccag
    19141 cccgaccatc aggccgacca tggcgatatt cgtgcacagg gcgtagttcg ccacggcttc
    19201 cgtgtccatg aagtagacca ggacgtagcg gtcctgcacc atgaacaacc gctcgccgag
    19261 gatcaggggc acgaggggca cgccgaagcg aaggacctcc gccagttcgg cccgcgacgc
    19321 gcgggcgggc cagacccggt cgaaacggat ccagcgctgc atgagcgccg cggtcaacgc
    19381 gagccagatg acccatatcc acaggatgtg ccccacctgg aggcggcccc accagaaaaa
    19441 cgggatgagg ggaatgaacc aggtgtcgcc ctggagcagc tgcgcgatgc gcgacagggc
    19501 gtacttggac aggcccagga agtagtggaa gtactggttg agatgcaccg tcagcaccag
    19561 cagcagcccg caggccagga tgtcggaccc ggagaggcgg atcttgccca tcggcagcca
    19621 cgcgagccag cccatgcccg ccagcgccag cgccacggac agcccgccga aatacagcag
    19681 gatcgtcttg aacatcccgt tctggtagtc gtccggccgc cccgggattt tgcggaggag
    19741 gaattccaag cttcccaggg aggacaccat catgacgaac gtgacggtgt tgttgatcag
    19801 cgtcagcgtc ccgtactgct ccttgctcaa cagctccgcg aggatcttca gccggatcgg
    19861 gctcaagatg aaccgcaagg ccacgaagcc gaacgaggcg aagaagatgt tcaccaggtg
    19921 cgcccagcgc gacggaatcc ggcccgcctc gacttcggat tctgccagga actggttcac
    19981 ggtcgattat gactaccgga tacccgccgt tcaggccaga gcaaaagcgg cgggctcaaa
    20041 cggggctaga acacccagcc cgcgctgaac agctcggcct cctcgtggac cagcgccgcg
    20101 ctgacggcga gccagaggga ctcgaaccgg taccgcgcgc cgatccgcag gctcgtcttg
    20161 tcggtctgct cgatgtccag cgtctcctcg gcgaacaccg atccgtcctc gagcagggtt
    20221 gtgtagtggt agttgccgtc ctcgtacgcc agcgtttcgg ggcccgcgta cagggtgaat
    20281 cccccgcggg tgtaactggc gaccaggccg aggatcagct ccgtgaactc gagcgatcgc
    20341 tcgttggtgt ggctcccgat gtgctcatcc cacaggcgct cgtccgagaa gtcctccgtc
    20401 gcgtaggaca actgcccgta ggccgagagg tcgaacccgt tcttctccca caggaggccg
    20461 cgcaggcccg cgccgaaggt cccgccttcg ccggagatgt ccgggtgatc gctgttgcgg
    20521 gcctcgtcga tcagggccgc cacgaggaac acgtccagcc tcgggcccag cgccagcgcc
    20581 ccgtgcaggc tgaaaagatc ccagctgaaa tcgggagtgg tcaggttctt gtacacggtg
    20641 tcgatcgtgt agttcgccgt gaaattctcg aaccccgcgc cgacctgcca tccatccgcc
    20701 gtccggctcg aaacctcggc cagcatctgc ccgtgcgaca gggcggcccg gagaagcagc
    20761 gcgccggccg cgaggccgat acgggatggg cgtttcatga gttaggctcc gtttaatccg
    20821 acccttggct ggccataggc gcgggaatat ccgggaaatg ggaggggggt gtcaacagcg
    20881 gagcgaccgg ccggttatcg cttgatcccg gccggggttc cgtatatacg gtaccccccg
    20941 tggtgaatcg gcatccgacg gactgcaggg aagcgtcgcg gacgccgggc cgatccgccg
    21001 tagaggagcc cgatccatgt taaagcgctt tcttctgatt ttcctgctgc tggcgggcgt
    21061 cggcctgctg gccggtgtca cgggttgcga ggacagcaac gacggcggcg atgatgtggt
    21121 cgatgtcgag ccgaccgata actcggatga ttcggcggat gacgacgccg accaggccga
    21181 cgacgacagc gaccagtcga atgacgatgc ggcggaccag caggacgatg tcacgccgac
    21241 tccggagccc gagcccgagc ccgaacccgt atccgtcaac ctgaccggcg actggcagcg
    21301 gcccggcctg ggctccatct accgcctgaa ccagagaggc gtatcgctcc aaggcgccta
    21361 ctttgagttg gaagacccgt atgtcagcgg cgacatcgcc ggcaccgtcg tcggctcgat
    21421 catcgagatg gatgtcgccg tccattacac ggacggcatc cggaccgatt tcgtcgccca
    21481 caagtccggc tcgatcatca gttcggacca tatccggctc gtggtgacgt ccagcccggt
    21541 ggcccagggg caggtccaga actggtatcg ttgacgccgg atcagaggaa attcggcagg
    21601 acggcggcca gctgctgcgc gatggattcg tgaccggccg cgttggggtg gccgtccgcc
    21661 aaccggtcct gcctggcgat ttccacgggc acgatcggga cgtcggcgtg atgcacgatg
    21721 tccgggagcg gtgagttctc gggcagcaga agccgggcca ccacgatctg gatgccccgg
    21781 tcccgcaact ccctccgcat ccgggtgagg cggagctgtg cctcggccca ggctttctgg
    21841 tctctatcct gccacgtgag ctcgtgagcg agctgcgggt tcttcttctc cagcgtggcc
    21901 gtccaggccg actggtactt catgttgatg aactggtaga ggtggctgtg ggtctgcagc
    21961 catcgcttgc ccgggatcgg cagggtggtc ttcttccgcg gcaccaccag gtacccgcgc
    22021 tccgcgtgga acgagggcgc caggtcgttg ccgaccagcc ccacgaccac caggtgcggc
    22081 ttcaggggga tgaccttgtc cgcgatttcc tggcagtact gtatcaggtt gtatccggac
    22141 accccggcgt tgatgacttc gacctctctg aaccccgccg ggggcggcag ctccgtctgc
    22201 agccgggccg gccacgcctg ttcgtacttc actcccatgc cgaaggtgaa cgagtcgccg
    22261 acgcacacga cccggaacac cccgggccgc ggctccgtcg agcattccgg gccgcggaac
    22321 cccatcgagt tgacggcgac ctcgcttccg taggacgtgc cgtcgtagcc cggcttgagc
    22381 tcgtaggcca ggtcgggatc cgtcgattcc acgtacattc cccgttcgta caggatgata
    22441 tccccgaggt gggccagccg gcacacccat tcgcccagcg ccagggcgac gaccagcgag
    22501 acgcccatca tcagcaggat gcccagccac cggaagcgct tccgcggctt cgggggggtt
    22561 acggaagtac cggtactttt ttccatcccg tgctctccgt gaattcgcgc gcgctcaaac
    22621 accagatgac cagcttcttt ccggacaggt acgccgggtc ggccttgatc cggcggtaga
    22681 agttgatgcg cgacggcgtg gcgcccgaac cgcgcacgcc cagcacgtcc acggccgtcc
    22741 cgagctccag cgccagctgg tcggccaggc cggcgcccgc cgcctgcatg tcggcgcccg
    22801 cgtggaagat caggttgtgg ctgtcgccga ggaacacgag cgggctcgcg cggtcgtccg
    22861 cgggcggcgc cccggccgcg tcgcggacga agcggagcgg gagcttctcc ctgggcaggt
    22921 ccgggcggtt cagcgacgac cagaggtcgc ccgtgatctc cacgtcccgg gtctccgggg
    22981 cccagtcgcg ccggggcagg tccgtcgccc agggccgcga gaaaatctcc ccggcgagct
    23041 tccgcgccgc gacgatgcac gcctggccgg accagtgcgt gtcctgcttg cagtacagcg
    23101 ccgggccggc ggccttggcg gcgaggaagt cgtccgtcag gtccagcacg cacacgccgg
    23161 cctggcggag ctgctcgtag aactcgcggt ggaactcgtc cgcgcgcccc gcgggcgggg
    23221 cgtcgctcac gccttcggga tagatgacag ccttgggcgg caccgggacg aagatcagct
    23281 cgacgccggc cttcgcgagc tgctgctgga aatccacgat cgcgggcagg ggatcggccg
    23341 acgccgggtc ggtggcgcgg ctgacggccg ccgcgtcctc gccccagaac cggcccgcgc
    23401 cgaggtgccg cagctccgcc ggcaggaaca gccagccgcc cagcccttcc accgccgtcg
    23461 cgccggcggc ttccgccgcc gcggccttct ccgcgcacaa cgcgcgaaac gacgccgaat
    23521 cctgcgccca ggccgcgacg gcccacagtg ccgccgaaac ggcggcggat gccagttgtg
    23581 cagtgcgcat tactccgtaa cctctcccca gcagggatct tccagcatca gccgatcgtc
    23641 gtcaagttcg gtgcggttga tggcctcgta ttggacggcg acgtccgccc agttcttcac
    23701 ctgcagccgg atcgactgcc cggcccggag gcgcgcggcc ggttcccaga cgttgtcgcg
    23761 catgctccat gcgtagacca gcgcctcgcc gcccggcacc ggcccggagg gccgggcgat
    23821 gtccacgaga tggatcgcga cgatgtggtc cttgtagggg acgctcccgg gcttcggcac
    23881 gggcgcgatg gccgccacgg tgccggagag cgtctccacc gttcccggcg ccggcacgag
    23941 gaaggtcgcg ggcggcggtt ggccgaggga cagctccacg ggcttccagt ccccgacggc
    24001 cagctcgcgc gcggcgaatt cccagaccac gacgcgcttg cccgcgaggc ggtcgcgccc
    24061 gcgcgccagt tcctgcgcca gcatctggcg cgtcgcgtgg gcgccggcgt cgttgcgcac
    24121 gatgcggtcg accggccgct gcaggcagaa gctcaattgc tccacgaacc ccgcgttccc
    24181 gccccagccc atggagtcaa gcgagtagat gttgcagaag ctgtcgccga ggaacagcac
    24241 gtccgaggcg gcgtccgcct gccagaggag tcctccggcc gtcagcacgg gatggatcgt
    24301 cgccgcctcg ggcgggaaga gatcgctgcc ggcgggcagc ttcagcatca gcgcgatgtc
    24361 gccgtgattg gtgatggcct gcgcgtcccg gcggtacccc gccggcgccg cgtcgggaag
    24421 cccggcgatg tcccggagat acgccgccag cgcctccgcc gtgcgctcca tggattccgg
    24481 tcgccagtgg gtgtccgtcg cgaggaactg gggctcgccg gtttcccggc gcgcgcgcgc
    24541 cagctcgtcc gcggcgtcga acacgtgcgc gccgctcatg cgcagccgcg tgatgaagtc
    24601 cgcgtaggac gggttctgca gggcttcctc gcggccctcg taggcgcgcg agaaccgctc
    24661 cgggtggacc atgggcttga ccggcgccgg catgacgacg agcttgatcc cgcgcttcgc
    24721 cagctggtcc cggaaccggg cgatggacgc caccgggtcg gggtgggggg cggcttccca
    24781 ctcggaaccg cccgcggcgc gtttccggag ctgtttcggg tccaggaacc ccgggcccgc
    24841 caggtaatcg atctccggcc ggtagaacag ccagccgtcg cgcccgacgt acgtcttttc
    24901 attcccgacc ccgagccagc gcgtcagggc gtactgcgcg cggggcagca cggcctggcc
    24961 caggcgcgac gactgttcca actcgtcctc gtaggcgtgg atgtcgcgca ggagcagggc
    25021 gttcagggcg gcgacgcgcc ggaagaacgg gcccggccgt tccggccatt gccggaaggc
    25081 ccggacggcg cacttgaacg tgtcgtagca ctgcggcagc gcgtcggccc gccggcccgc
    25141 gcggtgctcg ccgatctccc gggcgtgctg ccacgcgggc acggcgccga tggtcgcgag
    25201 gaaggcgaag aacaggagcc ggaccaggcc gcgggaaata tccgtgtggc cgatctcgat
    25261 cttcgcgatg tcttcgcggc tcaatgtctg tttcggcgcg ctcatgtcag aatataaagt
    25321 aaatgaacgg gttgtatgcc tgcgtggaca gcacgacgat ggagagccac agcaggacga
    25381 tgacggccag ggccttccgt cccgtgatct tcctcgacca gtcccaggcc tgcggggcga
    25441 accacacgat gacggcgcac agggcgaagg tcagcaggta gtacggtttg tagaggatgc
    25501 cctggaggag gccggccccg tcctgcgcgg tcccgaggcc gagcatgctc ttgaggtagg
    25561 cgacggccgc ggggaggtcc ttggcccgga agaacaccca ggtgaagagc acgagaacgt
    25621 acgtcaggcc cacgcgcagc gggcggggca ggaagtgata gacgggccgc ttgttccaga
    25681 agcgctcgaa cgccagcagg atcccgtgca tcgcgcccca ggccacgaag ttccaggccg
    25741 cgccgtgcca gagcccgccg aggaacatca cgaggaagag gttcacgtac gtgcggaccg
    25801 gcccgtgctt gtttccgccc agcgggatgt agaggtagtc gcgcagccac gtggagagcg
    25861 agaggtgcca gcgccgccag aattccgtga tggactgcga gtggtacggc gagtcgaagt
    25921 tcttggcgaa cacgaacccg aacatcagcc cgagcccgat ggccatgtcc gagtagccgc
    25981 tgaagtcgaa gtagatctgg aacgagtagg acagcacgcc gaaccaggag tccgcgcagc
    26041 ccgccgagcc ggcattgaag accgtgtcgg ccaccttgcc gcagggattg gccagcagga
    26101 ccttcttcgc gaggccgagg gtgaaaaacg cgatgccgcg cgcgaacttc tccagcgtgt
    26161 gcgtccgctg catcatctgg tccgccacct cggagaagcg gatgatcggg cccgcgacga
    26221 gctgggggaa catggcgacg tagcaggcga agtccgtgaa attgcggatc gcccgcgcgt
    26281 cgccgcggta cacgtcgatg gaatagctca tcgactggaa cgtgtagaag ctgatgccga
    26341 ggggcagggt gatcttgaac gcgaggtcca ggcggaggtt cgggaacccg agccagcccg
    26401 cgagggcgtc gtagctctcc gtcgcgaagt tgaagtactt gaagaagccg agcagggcga
    26461 ggttctggac gatggagatc accacggcgg ccttctggcc ccgggatcgc gggctgttcc
    26521 gctccagcag cgggacgggt tgggaccagg cctcccggcg gaactggcgg gcgagctgca
    26581 ggccgcacac gtaatcgatc agcgtcgagt acagcatgag caggacgaaa tagggattgg
    26641 cccagccgta gaagacgtag ctggcgatgg tcaggagcag gtgcttcccg cggcgcggca
    26701 tgaggtagta gagggccagc gccgccggca gaaagtagaa gatgaagaga tgtgaactga
    26761 agaccatcta gttgccggtt gagtaaaaga ggggcaggtc gaactcttcc gtttccatga
    26821 agagtcgttc gccctccagt tcggggtgcg cgtcgaacag ctccagttcc aggggatacg
    26881 tgccgccctg ttccttgtcg aggtcgagga cctgcttgtc ccggatcgcc cagtgggcca
    26941 cgaggatgcg cggggcggag cattcgccct ccaggacctt gacgacctcg taggtgtaga
    27001 ccaccagcgc ctgcgtgtag ggggagatcg aggccacgga gggcgtcggg atgctcgcgg
    27061 ccaggcgcgc ctcgaccttc acgcgcgtcc cgggcttcgt ggccgcctct ggcgcctgcg
    27121 ccggctggcc ggcgggcagg gtcacggcgg cgccgcggcc gccctcgatc cacaggtccg
    27181 ggaagaaatc ggcctggtcg gcgcccgtga gtttcagcca gtcctccacg gccgtgaaat
    27241 cggggctgaa ccggccgagg tacacgtcca cggcgccgcc gcccgcggcg atcaggttct
    27301 tgccttgcgg cccgaccttg taggggccgg tcatggcgaa gtagcgggcg tggttgctcc
    27361 agcgggggtg gtagacctcg tagccggcga cgccgggcgc gccgttgatg gtcaccttcc
    27421 agtggcggcc gtccggcgcg aacaggtgca ggttgcggtg cggcccgtcg aagatccacg
    27481 agaggtagct gttgtccggg gccatcgagg tccagcagcc cttgcccagg acggtttcat
    27541 caccgcccgg cagcctcacg tagccggcgc gggggtgggg gaactgtccg ctcgcgcgcg
    27601 tgccgtccgc cgacaactgg aagttgtccc acgtcacgtc ctgccggtcc aggaccacct
    27661 cggagaccgc ggggttgtcg agctggaacc ggatcacggg cccgctgtga aattcctcga
    27721 tctcccgaac catcatgtac acccactcga cgccggtcgc ggggtccagc catacgtccg
    27781 cggccgtgcc gttggtcagc gtccggaggc cgctgccgtc ccagcggacg acggaaatga
    27841 ggcggtcgcg caggttgttg aagacgatgc ggtcgccctt cggcgtgatc atcgggcgat
    27901 ggtagttcgt caccgcgccg aggatgacgc gttcgcccag cccgtcgccg ctgtcgtagc
    27961 cccaaacctg gagttgccgt ccccgcgcga agacatcctc ccccgcgcct tccgcctgcc
    28021 ggcaccagac cgcccgggtg cgcgcccccg tgaacgcccg gacgcgcgcg tcgagatcgt
    28081 ccgcctgcgc cgcgccggcc agccatgcgg ccgcaaggaa aaggcctgct accgtgtgat
    28141 gtttcatggg agagccgtag aaaaacatat ccgccgccgt ttctcaagtc ccgatcaggg
    28201 ttgtgtcccg atcaggggtg ggcatcgaag gcgggccgca gggcgttttc gaagctccgg
    28261 gccagcgtct cgaagccggc gtcatttgga tgcagcccgt cggccagcag ttttcttgac
    28321 ccggccagca actcggggcc ttccaccagg tgaaccgccg gccgttcggc ggcggccacg
    28381 cgtatcgcct ggcgatagtc ctcgatcagg cccgtgttcc acttgcgtcc gcgggcctcc
    28441 ggcggggtgg cggccacgat gggcagtccg gggcatcgcc ggatcagttc ctccaggaag
    28501 gcttgatagc gctcggcaaa cacggccagg tccgttccga gcaggacatc gttctccccg
    28561 atcaggacca gcacggcatc cggcttcagc gaggcgatgg ccgcggcgtc ttcgggcgtg
    28621 gtgatccggc cgcagaaacc catgttcatc acgttccaac cggtggcccg cgccagttgc
    28681 gccgggtagg aacgcgaggg gcgggtggca taaaagccct gcgtgatcga atctccgtag
    28741 gcgaccacgc gccggccctc gaccggcggt tgccggcgca cgcgggcccc ctcgttcacg
    28801 gccaggccgc ggaactccac ccggtcggcc agcggaaggt aaatctcatg tttccggacc
    28861 cgcggcgggc cgtccagcgc cagctccacg tcctgcgcgc cgcccccgcc ggacgcccgc
    28921 gtaaaggaaa gccggggctc cccgtccacg atccattccc cctcgctcaa gaaataccgg
    28981 tggtccgcca tgtcggcctg gtcggcatag gacaccgtga gaacgagacg ggtggcgtcc
    29041 gtcacgaatt ccaggcgcat cccggcgctg tcggccgcgt agggagagcc ggcgacctgg
    29101 cggcgcgcgg cggcgggctc ggccgggaaa tccacggccg ctgtctcggc gcgacccgcc
    29161 gttgcggcca ggaagccggc cagcaggaac caagctgcat gtcggggccc catcgcgcag
    29221 gagattctag catcgcgccc ccggattgtc gaagccgcat cgcctgccct tttccgcccg
    29281 ggccgaggtc cgcgttgaca gccggggttc cgcgtgacat gataaaaaaa ctctgaatcc
    29341 ggagggcaca ttcatggcct cattgatcgg catgtcggcg gaggtaaaag ggaagagttt
    29401 cccgatcgac cgggatgaaa tcacgatcgg tcgcgccaag gacaacgtcg tggcgatcga
    29461 aagcccgacg gtctccgggc atcactgcgc catcgtccgg gacggcgccc gctacctgct
    29521 caaggacctg gagtccacga acggcacgcg cctgaattcc aaggacgtct cggaagccaa
    29581 gctgcgcccg aaggacatcg tccagatcgg ctcggtcgag ttcctgttcg acgccgagca
    29641 gacggaggcc gtggataccc acagctatgc cgaggcgcag atcgaggtcg cccccggccc
    29701 gaccgccgct ccggagtcgt tcaactccat ctcgccgttc ggcgcccgcc gcaccgagag
    29761 ctccggggtc ggcctgctgg tgatcacgct ggtggggctc ctggccctgg tcatcgtggg
    29821 cctgttcgtc gtcaagctca tcttctccga ttgatccccg atgcgcctgc cgcgacggag
    29881 aaggccggcc ggtttcacgc tgttggaact cctggtcgcg atgctgatcg cgggcctgat
    29941 cgtgggcgtg gtctacaccc tgtatcatac cgtcacgctg gccgtgtccg gccgccgcgt
    30001 ccgcgacgac caggtcgcgc gcgtcgggtg cgccttccag tccgtgtgcg cggacctgtc
    30061 ctccctgttc ccgggcccga gcgacgatgg cctgatccgg ctgggattcg ccgaggccgg
    30121 gcagaccaac ttttccgacc tcgtcttctg cgcggtccgg gcgcccgggt ccgagccgga
    30181 catgaggtgg gcccaggccg tccaggtgcg ttaccgcgtc gatacgccat ccgccggggg
    30241 cggcgaactg ctttgcgaaa cgcgggcgct ggccggcccc ggcgcggaag cggtggagac
    30301 caaccggttg ctggcgggcg tggcgggttt ccgcgtgagc ctgttcgacg gggagacctg
    30361 gaaggccgag tggggcggga cggcggagcg gatcgccccg cgggccgcgc ggatcgaatt
    30421 ggaaacggac ggaggaaaag aactcgtccg caccgaggtc tcgatcgccg cgggcctcgt
    30481 cgtcagcggc cggctggagc gggccgcggc cggcccgtga gtcggaacgc cggccccgct
    30541 tcaggatccg cccgcttcgc ggaccatgtc gaaaaactcc tcggcggaca cggtctcgat
    30601 ccgcttcccg cggcggcgaa gcttttcgtt gatcttcatg caggcgccgc gcagcgactc
    30661 gtgcgtctcc tcggatccca tcaggacggt gaaattcggg ccccgcgtaa tccgcacatg
    30721 ctcgtcccgg tggtcgagtc ccacgccgag catgtgcgct ttcgcttgtt taccggccat
    30781 ggcctgtgca tcctcgatcg tcgccatgca gaacatactc ggcgggcgcg cgcggggcaa
    30841 gggccgtgtc cccggcctcc tggaggacgc gtaccgccgc tatcatcgcc cggaatgcat
    30901 acgccccgac ccgctggagg tggcgctccg ctacgccgat ccgcgcgacc aggagatcgc
    30961 ggggctcgtg gccgcgctgc tggcgtatgg cggcgtgaag gccatcatgc ggagcgccac
    31021 cgccgtgctg gcgagcctcg gcccgtcgcc gtcgcggacg ttgcggcagg cggacccacg
    31081 ccgctggagg tcacggcggc gtttcgccgg ctgtgcccgg aggaccccgt gcgctgggat
    31141 ttcgcgctga cccgggcggc gatatccgag cgcggcggtc gatatcccgc ccgacaaacg
    31201 gattgcgcca gagaaggctt gacggtccct tgccgttaac ctatcaaata ctgtccagag
    31261 gtataccacg cgcgccgtgc ggtcgtagta gatggtgtaa cgagccggtg aggcctttat
    31321 gacgaaacta tgcgcccgtc gttcccgtgc cgtttgggtt ccctcctcgc tcctcctgct
    31381 gaccctgctg gtccccggcg tgacggcgca ggaactgaac gtggaccagg agttcgagtt
    31441 cgcgcgcgcg ctgatcccgc tgggcttcgc ggactactcc gacaaggtcg tgcagaacgt
    31501 gctccgcctg cacccggaca tgaaagaccg cgcgacggtc atccaggcgg agatcctcgt
    31561 gtccaagcgg aagtttgccg aggccgagga aatggtgaag gccatggacc ccgccagccc
    31621 gaaggcgcag gccatcagcc tggcgatcgg caagggctac tacctcatcg gggagcagga
    31681 caaggctcgc gggatcttcg aggccttctt caagcagtac gaggggcgcc tgcccaccga
    31741 cgccgacttg ctgacgttct accgcgaggc cgcgttccag ttcgggcaga tgctggagat
    31801 ggccggcgac ctggcgggcg cggtggccgc ctacgacaag tacctgcaga ccaagcccga
    31861 tcccaacgtc gtgcgcgcgc tgatggccaa acaggccgag gcccacgtca aactggcgga
    31921 gagcctgacg ggggccgagc aggagaccca cctggtcgcg gcggagaagc taagcacgga
    31981 actgcagtgg aagggcctgg acatctggtt cggccagtcg ctgatcacgg tcgcccacgc
    32041 gcacctcatc cgcggcaagc cggccgacgc gcagaagttc ctgacgcaga acatggacat
    32101 cctcaaggag atcgacacgt acatccgcga caacgacatg tccatgtcca tgagcccgat
    32161 ggcgggcgcg cggttcctgc tgggcgagct ctaccagaag gacgccgagg ccctggcggg
    32221 cgggggcaag aaggacgagg cggtccaggc ctacggcaag gccatcaccg agttcatcaa
    32281 cgtcttcgcc cagtacagcg acagcgactg ggggccgaag gcgggcctgc gggcgcagga
    32341 gatcaagaag atcctcgtgg agcagtacgg caagcaggtc aagtggaaca tggacgagaa
    32401 ggccgtcgcg caggtcagcg aggcctcgtt ccggctggcc gataacctct accttcagaa
    32461 gaaatatccc gacgcgatcg gcgagtacac gaagatcctg aagcagtatc ccgagggcga
    32521 gcccagccag aaggcgctgg cgaacctgct ggactgctac atccagggca acgacgcgct
    32581 gatgatcaag gcgacggccg agtacatcgg cgagcggttc ggcgggaagg acatcccggc
    32641 catggcgctc ctcgcggcgg gcaagacgta cttcgaccgc aaggacgagg cgatgtacac
    32701 gtacgtgtac gagacgtacc tgaagcactt cccgaagcac aaccgcgcgg cggcgatcct
    32761 cttcaccctg gccggcctgc gcaagggcgc gggcgacgag gcgggcgcgg cgaagtacta
    32821 cgagcggatc gtcgagaact acccgaagga ccagtactac ccgaaggcct tgagcgccat
    32881 ggcctggggc tacttcctgg ccacgaacta cgagggcgcg gtcaagggct tccagctctt
    32941 cctccccgcg gcccaaccca accccgacaa ggtccaggcc cagttcgcgc tggccgaatg
    33001 cttccgcatg ctcggccgct ggaccgaggc gctcccggaa tacgagaagc tcatcggctg
    33061 gctcgtgccc aaggacaatc cctacgccac gtccgcggcc gacgcgaaga agaacgccga
    33121 catcctcgag aaggccgtgt tccagcgcgc gttcgcctac tcgcgcatgg gcggctcgcc
    33181 cgacgaggtc aaggcgaacc gggagaaagg catccgcgcc tacgaccagt tcaccgcctc
    33241 gttcccgaat tccacgctgg cctccaaggc gctcgacgcg aagggccgca tgcagctcga
    33301 gatcggccag ttcgacgcgg cgacgaagac gttcgacgac ctggccgcca agttcccgac
    33361 gacggacgag ggcaagaacg ccctcttctc cctgatccgc agcgccgtcg aggtcaagca
    33421 gttcgagcag gcgctggcgg ccttcgagaa gatgtacggc cagcgcgaca agttctcgcc
    33481 ggacgagttc gcccgcgtcg gccagctgat gctcgacggc gggcagtacg cgcaggcggt
    33541 cgaggccttc cagtacgtca cggtcaaggt ccccgaggcg gccctcgacc cgaaggagga
    33601 gcgggcgctg ctggagcgcg cgctgttcgg cctcggccgc gccgccttcg agcagggaca
    33661 gtttgaggcc gcctcgaagg ccatggacga cctgatgacg cgctatccca agtcggggac
    33721 gttctacgag gccaagttca tccaggcccg cgccaacaag gagctcggca aactgcccga
    33781 ggccatcgcc gcgctgggcg acatcatgaa gtacgcggac gacacggtgc tccgcaaccg
    33841 gaccacctac gagctggcac tgatgcagaa gcagaagggc gacaaggcgg acgcgctggc
    33901 ctcctacctc cgcgtcgccc tgctggccga cccgaagaac cccgcgctga ccgagttggt
    33961 cgagaagagc ctgatggaga gcatcgatct cgggatggag atgcagcgct accaggacgt
    34021 gctggacagc tgcgagcagt acatgcaggc gttcccgcag ggctcgcgga tcgaggacgc
    34081 gcgccgtttg cgcaacgagg cgcggatgaa ggccgccgcc gcggcggccc ggccggcgtc
    34141 gaccaatgcc ccggccggcg ggaagtgatt tcataacgat ggaaaggaag aagcccatga
    34201 acacctatcg atcgaagagc gcggcgtgga agctggccgg gctgctgctg acggtcgcgg
    34261 tcgcggccca ggcggcctac gtgatcctgt cggacaatcg gcggatggag ggcacggaaa
    34321 tccgcatgac ggcggatggc tcggtcgtgc tcacgaccga caggggccct gtcacgctct
    34381 ccaaggggca gttcaagctg gccatggccg acaaggtgcc ggcggaatgg gagaaggtcg
    34441 ggcaggcggt ccaggccaag aagtacgacg acgcgatcac ccagctgaac gagctggcgc
    34501 gcaagtaccg cggcctgggc ctcgacgtgc aggcgatcca gatgctgtcc aaggtgcaga
    34561 tgatgaaggg cgaccccggg gcggcggtca cggcgatcga gacgctgttc cgcatgaacc
    34621 cggcggcgaa gggatcgccc gaactggcct ggtcgttccg ggaggccctc ctgggcgcca
    34681 agcagtacga ccgcctcaac acggagctca acgaggtcgt cgcgagcggc tcgcgcccgg
    34741 acgcggcccg cgcccagatc atgcgcggcg acatcaagat ggcccagggg cagggcgagg
    34801 gcgcggtgat ggactacctg cgcaccgtgg tcttcttcgg ctcggagcag gccgtccagc
    34861 ccgaggcgct gttcaaggcc gcggacacgc tggagaagct gcgcgacccg cgcgccaagg
    34921 agatgttcaa gaagctggtg caggaatacc cgagttcgcc ggaggcgcag caggcgcggt
    34981 cccggatttg agacgaggat gcaggcaatc gaacggtcac gaggaaagaa ggagaggaac
    35041 atgtcgagca aatacctggt gtgggcgatg ctggcagtca gtctggtggc gtggggcgtg
    35101 acggtatggg cgcaggagcc ggcagcggcc ccggcggctg aaccggcggc ggcgccggcc
    35161 ggagcgggcg cggacaatgc gctggcgttg agcgaggagg ccaaggcggc cgaggaggcc
    35221 gaagccaaca agctggatac cggtttctgg gcgatgatca tctccagcgg cgggctgggc
    35281 atcacgctgt ggctgtcctt gctggcgacc tccatcgccg cggcgggcct gacgatcgac
    35341 tcgttcatca acatccgcga gaagaagatc gtgccccagg cgctggtcac cagcgtccgc
    35401 gagtccatgg agcagggcga tgtgatgaag gccttgaaga tctgcgagga cgcgccgggc
    35461 tcgctggcca atattctctc cgccggcttc ggcaacgtga aggaaggctt cgacgtcatc
    35521 caggacgtcg tcggcgtggc cgccgacctc gaaggcgaga aactgctcca acgcgtggcc
    35581 tatctcaacg tctgcgccaa cctggcgccg atgctcgggc tgctgggcac ggtgcagggc
    35641 atgatctatg cgttcgcctc gctggccacg atgaccgcgg gcgcggcgca gcaggcggcc
    35701 ctggccctga acatctccca ggccctgtgg accacggcgg cgggcctggt cattgccatc
    35761 ccggccatca gcttctatac cttcttccgc aaccgcgcga cgaagatcat gctggggatg
    35821 gaaggcctga cgatggacct catcaagtcg ctgcgcaacg tcgaggtcgt ggaggaatag
    35881 ccgcgggggc gccgcgggag gcggcctcgc aaccggaggg aaaccggcat gagaaaacga
    35941 tcggaagagc cggggatcaa catgacgccg atgatcgacg tcgtgttcca gctcatcatc
    36001 ttcttcgtca ccaccgtgga cctggagtcc aaggccatcg acgagaacct caagatggcc
    36061 atggcccccc atggcgtggc ggttgagaag aaggacccgc gcaccgtctt cgtggacgtg
    36121 gactccagcg gggcgatctc tatcgcccgg gtgcgcatgt ccgcgccgat gctgcggctg
    36181 gtcctgcgca agtccgtggc cgagtacggc cagaccgtcc ccgtggtcat ccaggcggac
    36241 ggccgcacga agcacgagca cgtcaaggcc gtgatggact cctgcgcggc cgccggcttg
    36301 tggaaggtca agttcgccgc gaagaaggaa gaagcgcaga aacccaagtc gtaggggaag
    36361 ggagcaagcc atggcccgaa aacgccgaaa atccgagatg ccggcggccg acctgccaat
    36421 gacgccgatg atcgatgtcg tcttccagct cctgatctac ttcctcgtga ccatcaagcc
    36481 cgtggacgtg atcgcccacc tggacgtctt ccggccgtcg ccggaggcca gccaggagaa
    36541 gccggatacg ccgccgaaga tgatcaagat cgtgatcttc ccggacgggt tcgtgatcaa
    36601 cgacaagccg gtggacctgc cggacctcga ccgcctgctg tcgaggctgg cggccatcga
    36661 taccaagcag accattctca tcatggtcag cgcggcctcg ccgcacgaga agctgatcca
    36721 ggtgctggac ctgtgctcca agtcgaaact ggtgaacctg tcggtggtca gcacgaacta
    36781 ggtcgccccg gagaggatat ttatgaagaa gctggacgct aaagcgatcg cccgctggat
    36841 caaggcccac atctgggggc ccacgggatc cgtcatcatt cacatcctca tcgtcctggc
    36901 gctgctcaag ctggtgaccg gcacgtcgag caacaaggcg ccggagatcg aggtgatgat
    36961 gatggagccg gacgccgtgg acctggagga gttcgagcag gagctggaga agctggagga
    37021 gcttccggag atggtggacg ccgtcacgcc gcccgaggtc tcgctgacgg agcagccgcc
    37081 cgacgtgcag gccagcgact tcgcggcgcc gcagcccaac gtggacttcg ccgcgctcga
    37141 cgtgcagcag gacttccaga gcccgctggt catgcagggc ctgttcgccg ggcggtcgtc
    37201 cggcggccgg gcctccagcc tccgcgagta cggcgggcag tggggccagt acaccgaggc
    37261 cgcggtgctg cgcgcgctgg aatggctgaa gaaccggcag aatcccgacg gcagctggaa
    37321 gggccggctg gatccggaca cggtgggcta caccggcctg gccatcctga ccttcctggc
    37381 gcacggcgag acgacggcct cggagaagta cggcgccacg gtggagaagg ggatccgcta
    37441 cctcatgggc cgccaggagg agaacggcgc gttcttcaag aagttcgacg gcagcggccc
    37501 ggtgtacgcg caggcgatcg cgacctacgc gttgagcgag gcctacggcc tgacccgcat
    37561 ccctgcgctc aagcccgtga tggacaaagc cgtcaaggtg ctgctggacg gccagcaggc
    37621 gaaaggcgga tgggactaca gctacgccaa gggcgcccgc cgcgacacct ccgtcaccgg
    37681 ctggcatgtc caggccctga aggccgccta catcgcgggg gccgagacgc cgggcatcca
    37741 cgaggcgatg gaaaaagccc tcatggacct gcgctccgtc caggacccgg aaacggggac
    37801 cttcggctac accagccaga ccgatcgcaa gtcgcccaag tcgctcggca tgacgggcgt
    37861 cgccgtgctg tgcatgcagt tgatgggcta cagcagcgat tcggcggcgc gcaagggcag
    37921 cgacgtcctg tcctccgcga cctgcgactg gaacgatccc atgaactcgc cgttctacgc
    37981 ctggtactac atcacccaga gccggttcca ccgcggcggc gcgggctgga gcgagtggaa
    38041 caacaaggtc gccccgaccc tcgtgcgcaa ccaggacccc aagggcttct ggccggacag
    38101 caaggcggac aacgtcgtcg gcgagcgcgg cggcgcggaa agcgatccgc agacgatcta
    38161 ccggacgacc ctcgcggcgc tgacgctgca ggtctattac cgcctgctgc cgacctacaa
    38221 ggccgtggcc atcgagccct cgaccccggc cacgaaggaa gacatcgccg tcgagatcct
    38281 gtaacgtccg gcgcttcacc gccgagggcg cagagcacat cgtttctttg cgcccttcgc
    38341 gccttccgcc gccgcagttt gcgcgggccg cgttccgtga tagagttccg ctcatgcccg
    38401 gccctgccca tatccggctc ttgagcgacg aggtgatcaa caagatcgcg gccggcgagg
    38461 tcgtggaccg tcccgcctcg gtcctcaagg aactcctgga aaacgccctc gacgccgggg
    38521 cgacccgcgt gacggtcgag atcgtcgcgg gcggccggaa gatgatcgcc gtctctgacg
    38581 acggcggcgg gatggaccgg gacgacgccc tgttgagcat cgagcgccac gccaccagca
    38641 agatccggga cgtggacgac atcgaggacg tcgccacgct gggtttccgc ggcgaggcgc
    38701 tggccgccat cgccgccgta tcccagttca ccctgaccac gcgcacggcc gccgccgagg
    38761 gcgggacgga gatcacggtc gccggcggga agatccggga cgtgcgcgac gagggctgcc
    38821 cggcggggac ccgcgtccag gtgcgcaacc tgtttttcaa cgtgcccgcg cggcgcaagt
    38881 tcctgcggtc cgaacagacc gagctgtccc acgtccgcca ggtctttctc ctctatgccc
    38941 tctcgcaccc ggccgtcggg cttgccctgc gcgtagacga gcgcgaggtg tggcgcctgg
    39001 ccgccggggc gaccctgaac gaccggctgc tggaattgtt cggccccggc acgatcagcc
    39061 agttgcggcc cgtggatttc cgggccgggg agctgtggct gcacgggtat gcgggcatcc
    39121 cctcgttgag ccggggggac cgttcggacc agtatttctt catcaacggc cgcccggcgg
    39181 gcgcgccgct cctgcacttc gcgctgtccg aggcctatca cacgctgctg ccgcgcggcc
    39241 gataccccgt gctgtacctt ttcctggagc tgccgccggg acaggtggac gtgaacgtgc
    39301 acccggccaa gaaggaagtc cgtttccgaa acacgggcgc ggtgcgcgac gcggtcatcg
    39361 agggcctgcg ccgggccctc ggcacgacgc ccgccgcgcc ggaggcggcg ccgatccacc
    39421 cgctcgcgtc cgtggcgccg ccggcggagg cctggctgcc cgcgatccgc cgggatcagc
    39481 cgccggcctt cgcgtatccg cgcctgccga tggcgcccgc ggaaggcgag gcgccggcgc
    39541 ccggcccgtc gggatcgccg gcggcggccc cggcggccgg gccctgggca tggtgccgcg
    39601 tgctgggcca ggtgggcggc ctgtacgtgg tcatggagac gcccgacggc gtggtgctga
    39661 tggacccgca cgcggcccac gaacgggtgc tgttcgagcg cttcatgaag gaggtgacgg
    39721 cccatcgcgt gaacagccag ggcctgctgg cgccggagac cgtcgccctg ctgccgcagg
    39781 atgcccgccg ggtgcgcgag cagctcgacc tgctccgcga catgggcttt ggcctgtccg
    39841 agttcggcgc cgacaccttc ctggtggacg ccctgccggt gtgcctcggg aacgtttccg
    39901 cggccgcgtt gctcgccgag gtggccgaca ccctggagcg cgcgggcgcg cgcggcggca
    39961 cggagcgctg ggcggaggag gccgtggccc aggcctcgtg caaggccgcg gtcaaggcgc
    40021 gggaccagct ggcccccgag gaaatcgagg agttggttcg cgcgctggcc gccacgggca
    40081 tgccctatac ctgcccgcac ggccggccga cggtgatctt catgagcttc ggcgaactgc
    40141 accgcaagtt cgggcgggcc tgatcaggcc agggccgacg cgaccgggag ttgcatcacg
    40201 tcctcccgga aactttcgta cgccagccgg tagcgctccc gttcctcggg gagaaactgg
    40261 tcctggttgg cctgctgcag ctggcggtag gccgtgtggg ccagctgcag cacgttccac
    40321 agcccctgca ggatgtcctc ggcccgacgt tggtagtgca tggccggcag gacgcactgc
    40381 tccggggtgg cggagaggaa ctggcagagg aagctctcct cgccctcgag gtgcaactgg
    40441 tgctgcatca tcgcgtgggc ggactggacg ctgtcgtgga tcaggcgcag gaaccggagc
    40501 atgtcgtgct cgcgcggcag ggcgcgcgat gcctggatgg cgaagaaggc gtgctgctcc
    40561 gaggtcttca gcagggacag gacggtctcg atgtgctgga tgcgctgcgc cgtcggcagg
    40621 cgctggaagg gggtgtagag cagttcggcc gcgcgctcga agaacacgtc ccgccgggcc
    40681 aggtgcaccg gccggttgat gtcgaaatgg ttcttcttca cgtcgcccgc ctcgctcttc
    40741 cgccgcagaa ccagcctatc acgcgtcccg gcccgtggca aggcgtccgc cgggcggtcc
    40801 ggccgccgcc ttttcacctt gaccccgtgc cgcctgcccg agcatgctgg aaaaccgtca
    40861 agggcgatct ggccgctcgg gaggagccgt cgcagggaga ccgggcccgc gccgggccgt
    40921 ccggatatgc ggaatgagga taaccgaaaa ggaggctgga catgcggcgt gtgcaagtca
    40981 ggcgatggac ggtactggcg ctcgggctgg cggggctggc gcagacgggt tttggcgccg
    41041 ggttcgccct gtatgagggc agcgcccgcg gaaacgccct cggcggggcg atggcgggca
    41101 gcgcggatga cgcctcggcg ctctactgca acccggcggg catcacacag ttggaaggcg
    41161 tgcaggtcat gggcggcatg acgctcatcg cgccctcgac aaaggtcacc accatgaccc
    41221 cggcgggcag cgtgacgacg gaaacggagg agaacctctg gatcccgccc cacctgtacg
    41281 ccacctggga ggcgagcgat cgcgtctggc tcgggttcgg catgtactcc cgcttcgggc
    41341 tcggaacgga attcgacgag aactggcccg gccgctacaa cagctactac gcccgcatcc
    41401 gctcgatcga gctcaacccc aacatcgcct acaaggtcaa cgacaagttc tccatcgccg
    41461 cgggcctcaa cgcaacgtgg ttcgagctga agctcgagca ggcgatttcg ccga
//
LOCUS       DAOOTK010000018        41450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_709_length_41450_cov_5.527044, whole genome shotgun sequence.
ACCESSION   DAOOTK010000018 DAOOTK010000000
VERSION     DAOOTK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 41450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41450
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_41450_cov_5.527044"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="PK634_03800"
     CDS             <1..2083
                     /locus_tag="PK634_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96890.1"
                     /translation="NGALGSVAAGTGVSSGAVLRLQGSVFYTASEPLDLAGTVAGYTG
                     SNTWAGPISLAGPAVFAASSFATLHLSGSIGGAEGVAISGGGVVSIGGAGSSSYAGTT
                     TVHSGTLNLGRSAGPAVPGALVVGGEEAATVRVGWMNQLAATNPVRVQEYGVLDLNSF
                     GLFNPLGQLRWRGGSVSSGNGPMNLVTDVVAEASALSAGLAGIVSLGATDRVFLVENG
                     AAENDMVVSAMLGGGGIRKTGGGRLWLGGGTFESPVRVAEGEVVAGADQALGLGPVFL
                     GEGSALGVGLYFGGNGRLTNRLEVTAGARILGTLPGVATGTWAGPMVVEGALTLQAAA
                     ASRLVIERGLEGAAGAVTVTGGGVVELHGAGTYPGGTVVAGGAMWACHGGSSAVGAGA
                     VQVQAGGALGGTGVVHGPVTLAPGATLNPGCSAGTLAVSNLVSLEDGSRFVVEAEALA
                     YDALDLRRGGAVALAPAAQLLVPGPLPGTDACVIVRGATQVNGIFSGLPSGTALPPPN
                     EDWYIHYKPTGIYLGRNAAPLWYFRGYANEGAAMLSWRTPVELGVLGYDLYRDEGGSW
                     VKVNGDPIPPRDPNGAAYTFEDPLMGPGMTGRYRLVELYDGGLSNELVDCAREFTELA
                     FTAGPRETDGGLELRWRSREDEAYWLERSSDPAQDYVPVAGPLPATPGENTYTNPVSD
                     TRGFYRAVLMP"
     gene            complement(2116..3624)
                     /locus_tag="PK634_03805"
     CDS             complement(2116..3624)
                     /locus_tag="PK634_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW96891.1"
                     /translation="MTFESSPERLEALDYISDGLLVADAGGRVVFSTPSIAGLLGRAP
                     ATLGELSALLKPDGGPSFDSVLNALEREGRWSGEATGHPPRGAERLLDIRAERLLDAK
                     ERTLGYVFVARDVARERLLVKQVMQAQQMELVEHLSGGIAHEFKNLLTIIMAYASLLR
                     DQMPSSDLQADVARIQQAAQAANDLTARLLAVARHSTPVLEDVDIHDVLGDIVALQKK
                     ALPRNITLSMPERETLPRVRADRATLDRALLNLCLNARDAMPEGGKLTLGLAEARLSD
                     QDIQSRPEQRPGHYLVISVSDTGPGIPPEIQDRVFEPFFTTRKAATGMGLTVVRHAVR
                     AMGGWVTLHSEPGLGACFELHLPTADTPSAAVSGPGPDEGPGPGGTERILAVDDDPLA
                     LSIAQRILQRAGYSVHGAPGGEEAIEYFRQRHAEIDLVVLDVVMPYVNGEDVYREIHR
                     IKPGIPVLLVSGFTPKTAERLIRITGAQFLTKPFTQRGLAQAVRDALDRNKA"
     gene            3774..5615
                     /gene="glmS"
                     /locus_tag="PK634_03810"
     CDS             3774..5615
                     /gene="glmS"
                     /locus_tag="PK634_03810"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOW96892.1"
                     /translation="MCGIVGYVGRRDAVPLLVDGLKRLEYRGYDSAGIAVMEKGRLEI
                     RKCVGRLSALEEKLARQPVAGAPGIGHTRWATHGEPSEINSHPHTDSTGRIAVVHNGI
                     IENFESLRHDLEKKGHPFRSETDTEVVPHLIEQELKDGARDLPEAIRRALAKARGAYA
                     LGIVSLDDPDTIYAARLGSPLIVGVGDDGQFIASDVPAILNQTRKVIYLDDGELAILK
                     AGGVRVMKLDGTEVKPDIREVKLQAEAAERAGFETFMRKEIHEQPRVLRSLLDKHTKD
                     AAAVALQDVGFDDAQWKAVRRIMIISCGTAYYAGSYGRLIIEALTGLPVEIELGSEFR
                     YRDAKLSPDMLVVSVSQSGETADTLASIRKARRAGCKALAICNTPGSSLARESDVVFH
                     TEAGPEIGVASTKAYTAQLMAFALLAIHLGRLRGELTAEQERELVRDLHAVPDAIHYV
                     LARGEEIRAIAQKHGDGPSALYLGRKFNYPTALEGALKNKEISYQHAEGYAAGEMKHG
                     PIALINEYLPVVCLCTKTRDEVYEKMISNMKEVEARHGRIIAVASNGDESVLKLAEDI
                     IFVPQVRDEFSPMVNVVALQLLAYFAAQARGTDIDKPRNLAKSVTVE"
     gene            5892..7532
                     /locus_tag="PK634_03815"
     CDS             5892..7532
                     /locus_tag="PK634_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96893.1"
                     /translation="MNPGWNAVYLEVQPDPRLLDEVFAGRAVEGVWKWDRRFSHIEYV
                     TDPCVLEPEDPHWLVWLPPEDSRRFLSRLFELQGGQAYLVKIPTNAAGYSLPVKGRVI
                     IPVTDWFAHGLNLVGFPVHTTYPPTFADFFRFTDEIDTTRGVNNQLFSINSAGRGMTI
                     VQPNRETIAAGRSYWVGCDSDPKYPGPLVVEPSRVGAVDFGSHVVQQELTIRNLGPTA
                     MTVRVTQEASEEESEGFPELAGAVPLSYMVRNASSQWEWIEFPVGGLSEVVAAGGAWT
                     IYLGVRRQDFVAYAPVGTNGALYQSILNVEDTALSLRVRVPVIAEKPAGLMGAELSDH
                     HESEGLWVGQATLDRVNAPAYTNGLLPTPAPLTVRLIVHVDAYGRSRLLQQAVLVWDG
                     SLTNAPHTNGTYALFVNDAEMPADAEEAYRISSTAFPLMPPLLLDGDFTNVLTGTVTV
                     DFDDPTNPFLHRYHPMHDNKNWDFESYTNAVETRTIARDVTLDFEESPTNLVHHPYWG
                     VDQILGTYRETMTGLRAQPIHVEGDFWMERISTINELQ"
     gene            7643..8509
                     /locus_tag="PK634_03820"
     CDS             7643..8509
                     /locus_tag="PK634_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOW96894.1"
                     /translation="MKTRLNAQWLLVILLYSSFILHPSAFSADVPPIINYQGLLTDNL
                     GNAVTAGYYEIEFRVWDSPNLTGAGHLIWGRSFPLHVMSNGTFNILLSNDGGAITNPV
                     SPVTNDLRGSFESTDRYLGLTITHNPLGAVGSPAEISPRQRLVSAPFAFHAQHATETP
                     GTIPIGGIIAWSGAVTNVPDSWALCDGGTYSGQVTPDLRNRFILGAGGVYARGDAGGT
                     NQVTLTIDEIPSHSHGGIRDKGENSAYHATETGGYFWKHDNWKNTDNTGGGHPHENMP
                     PYYALCYIMRVR"
     gene            8553..16757
                     /locus_tag="PK634_03825"
     CDS             8553..16757
                     /locus_tag="PK634_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOW96895.1"
                     /translation="MKAERRKARFSSRFILHPSLRILHRSSFILLFASFLLHPSSFSF
                     ASGYNLDTLYQNRWFEPSPAGGPYTNYFGAWPGGTVSNASTAIYNGHAMGNTNAVSPS
                     NASYRLASSAFGQPVEGIKADVALGDVIPSPPGANTNVPPANFVAVQVGGNSAAYYES
                     TDPPHAAFWSPSTKQVIAAQPDSVAVAWVMTNHTTNVVATYVGAVPSSRPARIFWTES
                     PYDAPRINLNGLFPVIHYSKTVPAPVYHVVTNVNGGVTNITSNVVSGVWIDGQKQMHA
                     KDTSGMFILEYYQAGTYKDEVQPDGIEVVQVLEPDLQVIWADIGSRLLPADSYWAQVD
                     GNGGIIPLVQVGLNETAYMVDQVGPKDNWVFSIKKTVYEPWSLEIYWQHRGLMGVLWP
                     YEKDWYSCDWPLHPQLYVFGSSTQDAAAVLIPDGLTAALMEYMDPAFHAEISTSGRSF
                     SASEPGRCLLKYTTHDDIWFEVVQTVSHTNSTYFDLDPNDWTIGDELTPGPEEAHALA
                     FDGTDDYVSLGTSWVNGRSDWTLALWFNPGAAAPGVLYSEGGSGGVTFDVVLQANRAL
                     SVGFWNKYHLTNHWVSLVTPTNTVRSNAWQYLAVTLSGADDSNGTARLYLDDRSWEAT
                     NVWIVNFDGSQLGLMAASPTSHGITNFFRGRLDEVRIWSEALSTGAIRSNQYDTSDGY
                     SGTQLANYSFDEGEGAVIHNWAGGYHGTAVNGPLWCYGQVTPGADYASFPGYVHVPEG
                     DRYNVHRYDYPTEANPGAESYVFAVNTNFLEVWWANRSRQDGMPALYYPSHVTRYRNL
                     WPTNAPHIVIASGLGSQGDQLVPATNALDFNGTSDYARAPTNPPALGMEGAVTVEAWV
                     QLDAAGDRKIVAKTDSGPLWKGLVLGVKSNACFPELWDAASNHYSCLAGSVPTGAWTH
                     IAFTYETGQYFRAYVNGDLMHQETAGGPISGSTSTVFIGRSSWETPYYVDGRIGEVRI
                     WSVARSASDIRGAMYTRLRGTESGLVLYYPFEPGESDAVLYDEGTNALDATISGADWV
                     ALGRPVAAAESFVFGSPSIYSQNTPGQPGYNPNEEHAMMLGGVAYALRNDLNSDLYSK
                     PFVLVDYLDPNTARPAMKAFEVVRSNTYYRFERSVTAGLPIVPLMPLAALPPCTNTTS
                     DTMPPAWRDRKLEWWARSAGDDGGPSNAILRFYYPIQPTFYFPGKATNAQPALDAEIP
                     WLPDPSSQNGAAGTPVPVTYAVTWPDDVPELKIAQTLTQPTHGLPDLWDQLSIEVVYQ
                     QSLQTNAGSCVTLFDPVAAQGAALSNDVVEAMIESGLATRDLVSDRVRFPGLTPSLYG
                     RVYYDPDRGTNGPLVLEGQYVTPLTGDGYLLLNVLEDYERNQAKSAAVGLEPADKAAW
                     DAAVDSLPVNVIQIQPNTPFVNGALYAGLSNGAGYVTLAFNNSTNGHQVPTALPVSLS
                     ILQVVPELYNGMLQVIEPEDPLDEQLSLRASPDFAGCLEDYEFQWRWEEPEGGLIPNT
                     NFPRWNAYGADTVEATNEITIAGASSFTLADHYFALRYRRTGGSGVTGTNWSEWTYSL
                     APGWIQRVLNGINPFTQCLEDMIENAVDVRSTMISLAGPPYEGDIALNLAAACEAGLI
                     PVYRTVLNRAEDFSIRAGVSDQADNEALLFAASRLHDLYMLLGHEAYADAQDPTISFP
                     RELYEDEHGAEATGLFCFMNQVPNLLEEELALLRGRDDTLEPLVTIGPVYNRLIWNFT
                     KGINGGEPAYAYNYNIRGTPTNTIGAITAEDAKRLYPQGHGDAWGHYLSALSGYYELL
                     SNTNFVWQTKPGATLLGNATVSSDFFDEQKFAEAAAAKARTGVEIVNRAYRLAYEEDA
                     TVRWAGYRDSNTNREWGLAGWASRTGQGVLYDWTVANSLLLDHLTNMTQVGGANEPPE
                     GIQKIDRSTVPELCEIASAADEVQRQLDHADYGMNPLGLDRDTVSFDISPTEIDEGQT
                     HFEQIYDRALQVLYNACVAFDYGRNITLKLREQFDSVYELQEQLAENERDFHNQLIEI
                     YGYPYPDDVGPGGTYPQGYAGPDLVNWQILDLEDLRTDAPTGQPFRVDLYNYVFNSGN
                     EFSGTNYSDYSNLAATNNFTTSVVGTITVYISDSGLKVKPPAWTSRRPAQGELQMALS
                     EMVQGWYALQAKKAEYNQTLYELKVELLHRQADYTRYPGEWSNSVENAERKKETARVV
                     EGLEITSSLLDIVASCLSDVQYMLAGLIPDQYDGLIGPFPHLGVNNHWSSADKVAIAL
                     SVYVRRLAIVAMQAGVTGREKLQERWDADLEKLISNNEYQDILHWATWETQAKLKEQF
                     VKQAEIMEQVQALNQQVEEVRKLLTQGQLLLTQRAQVRSRAAQRIQMNRYQDIAFRIF
                     RDDALRRYKDSFDLAARYVYMAAKAYDYETGLLDSDTRLTPGRRFIEDIVRARLPGRF
                     YVWLGTPETGIDEGEPGLADIMARMKADWEVVEGRFGFNNPDTETSRFSLRTELFRIS
                     ASSNSDGTWAQVLEDHRVDDLRELQEFKRYCIPFSDSTNTEPALVIPFSTLIVAGKNY
                     FGHNLAGGDNAYDPTRQATKIRSAGIWFTGYNVTFNTNTAGNGLANEPRVYLIPTGED
                     VMRSPTREGLETRNWKVLDQALPLPYDVGGMNIDNPDYQPIIDSLTEPLAQIRRFAAL
                     RAYHDRGQFDEAETHNNGRLIGRSVWNTQWLLIIPGRTLLADPEEGIQRFIHGAKVNN
                     VRDGNGIKDIKIFFQTYSIAGE"
     gene            16801..17187
                     /locus_tag="PK634_03830"
     CDS             16801..17187
                     /locus_tag="PK634_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOW96896.1"
                     /translation="MSVEKPMDLKGRLRDYALWIIRPYCSLSKRDDVAVVLGRQVLRS
                     GTSPGAQHREACRAKSTAEFISKMEGGLQELEETEYWLDLLEGSQRLSGDTVAPLMRE
                     TRELNAIFTASIKTAKRRKREQDESA"
     gene            17240..18013
                     /locus_tag="PK634_03835"
     CDS             17240..18013
                     /locus_tag="PK634_03835"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96897.1"
                     /translation="MPQPMVVYYGQAKDDFGWPYTRDATVILRSGTNDVALHSIDGSL
                     TPGINFALYAPLDDGRDTNRYVRNAVRTGEVVTIVVRDAWGERTLLETNSIPAVTTPG
                     DLICVNVTAGSDSDGDGLPDEWEQWLVDLSTNPAITSVADIHAEDDFDGDGVPNGEEY
                     RAGTFAFLDYDYFFIERSRLSAQGWWLIQFLSVPGKVYRLYEAYQVPTYPGDAWSPGV
                     YSTTDAGALGAGVIEGDGGWTSLYIPVTNSPRAYKMHVE"
     gene            18010..18816
                     /locus_tag="PK634_03840"
     CDS             18010..18816
                     /locus_tag="PK634_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96898.1"
                     /translation="MTSFFHRAALLMAVLSLAAGAAHADLVASDNADNDPYPSGWNPG
                     DNAGSGFTAWVSLQAGSPGSMYVENLAPLDGLYSWGLSGTYAVGRGLSNSLAAGQWNI
                     LASHGSDVGSFCGFNLRTSTDDSAFDTDEILRFGINYGEEFDATRIYFSTNGGADYAY
                     IDLGDADLRDSDLEYNVSWSTVSGSFTLGVRNGEDYEEVTADLPSDAAVAMFGAGIFE
                     ASLDERMTFDDYGVEAIPEPGALVTLVLGCGFIQWFMYRRLRRAARLLPF"
     gene            18863..20140
                     /gene="murA"
                     /locus_tag="PK634_03845"
     CDS             18863..20140
                     /gene="murA"
                     /locus_tag="PK634_03845"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOW96899.1"
                     /translation="MSRFIIHGQRKLSGEYRPVGNKNAVLPMLAAALLTDEPVTLRNV
                     PDIRDVEVMLALLEGLGVEVAREGDRVTLRAGSIRRRRLDPDLCRRVRASILLAAPLA
                     ARHGSCVLPPPGGDIIGRRRLDTHFNGLRALGIAVEGGAHYTLRRRQLRGADFLLDEA
                     SVTATENLLMAAVLAEGETTLFNAACEPHVTDLGALLVRMGARIEGLGTNCLRIQGVK
                     TLHGAEHAVGPDLIEVGSFLAAAAATRGELRITAPGNLRDLAVILRVFARLGVETIAE
                     AGDLLLPARQKRRVQRDLGRAIPKIEDGPWPQFPSDLMSVAIVLATQAKGTVLFFEKM
                     FESRMYFVDRLIEMGADIIPCDPHRVVVSGPCRLHGTRLATPDIRAGMSLLIAALCAK
                     GRGVVDQAEVIDRGYERIDERLRALGADIVREG"
     gene            20250..20702
                     /locus_tag="PK634_03850"
                     /pseudo
     CDS             20250..20702
                     /locus_tag="PK634_03850"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            20772..22148
                     /locus_tag="PK634_03855"
     CDS             20772..22148
                     /locus_tag="PK634_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOW96900.1"
                     /translation="MDQVIVVAYLVVVLVLGLWAGRGIRTLQAFAVVNRSYSSWVIFA
                     TLAATFIGGGFTSGNAAKVFLFGIANIVALWGFSFKELLIAAFVAPRSGAFRNCISVG
                     GIMRRQYGRTGQVITGLLGIMVCAGITGAQVGAMGTVFHTFVGTSREWGIAIGCGIVI
                     AYSTFGGMNSVVVTDILQFILLAISMPLALWLGIRHVGGTEALVQAVPAGHLSLFSHM
                     TPVALASLFLSFVLGETLVPPYVQRLLIGKNPRAVAEGSLFAGLFSIPFFAVTGMLGL
                     VAVAIQPDLDSNQAMPFVVNTLLPVGLKGLVIAGLISIVMSSADSFLNAAAVALIGDV
                     INPLRRQELSDRQGLWVAKIATLLVGLGAVLFAMKIENVLDILLFTYNFWAPVVLVPL
                     VATILGVKANRKTFLVGAFCGVVALSIWHWLLKDPQGIDGLVIGVAANLVAFSAMHRI
                     DVLTGPED"
     gene            complement(22157..23359)
                     /locus_tag="PK634_03860"
     CDS             complement(22157..23359)
                     /locus_tag="PK634_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOW96901.1"
                     /translation="MTGPDRPENIYEALLRVSLDINARLELTDVLEGVIRHTRLLLDC
                     EDASLVLWDARRRQFETGASTTRIGGTVATRVRQSGGATRWIVDHGQPCLVPDTRKDP
                     FTANPIIPENGIGAYAGVPVRQGDEVLGVLYALSRRTRGFSDEEVEWMKELAGMAAIA
                     IQNSRLMASLRELNEFKDALRRMAAHDLRQPLSPAIGYLDLALTGSPALPREHFALLE
                     EVRKALMRMRDLVSGILDYERVTEEGELVRQPVDLNAVANEALRPLRETAASRSQRLE
                     FSPVTVVPPVAGDLVLLRQAMSNLVANAIKYTPHGGRIAVATGMEDGSAFFQVRDNGP
                     GIDPEDQKRLFQPFTRLKSAGDTEGTGLGLSLVRKIVERHGGRVSVDSAPGRGSLFRI
                     HLPIAGNA"
     gene            complement(23399..25813)
                     /locus_tag="PK634_03865"
     CDS             complement(23399..25813)
                     /locus_tag="PK634_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOW96902.1"
                     /translation="MKFVGQSATRIDEREKVTGAAPYADDLDFGPNLLHAEIVESPHA
                     HAIIKKIDVSRARKVPGVVKVVTGKDFPYTFGLYMQDRFIFARDRVRFVGEQVAAVIA
                     RDPVTAKRAARLVKVTYEPLPFVLDLKEALKEDALLVHPDLKNYGHCAWFFPVPDTNI
                     AHWRKTRKGDIEAGFREADTVLEDTYTVPRYAHCAIEPHVIVGQCDLSGRLTLWSSSQ
                     SPHTQRHVFAAALAPLGLKHQDIRVITPYVGGGFGGKAGVSMEIIAAALALAVKGAPV
                     KVRWTREQEFYNTYQRQGVIARIKMGVKKDGSITAIEHTLHWDAGAYVEYGANVVNAV
                     GLSATGPYRVPNVKIDSVCVYTNLPPGGPYRGFGYSEFMFGLESHINRIAKMIGLDPV
                     EFRRRNAIREGDTLPYGAHMNPCALVDAIDKTAAEIEWGKKEEAAAPNKVIGKGFACF
                     WKAPAMPPNAASAAFLKFSEDGSVNISVSGMEIGQGYLTVMAQIAAEVLTVPPEKIRV
                     ETPDTDRNAYEWQTVGSHVTWGCGNAVKAAAEEARAKMIEVVHRVHGFAKEDLYLEDE
                     RVKCRAKPDFALAFKDFVINGIQTPRGNFLGGPILASGMFMPEFTSATGDPETSQGGH
                     PNVHYTVGAAGIVLEVDKETGKMRVRKVVLAVDVGQAINPDLVKGQVVGGMLQGLATA
                     LYEDIRYSPAGKLLNPNFTDYKIPTALDLPDAIVPIIIETPQPDGPFGARGVGEHTMI
                     PSAPIVANAIEDATGLRIKSLPLTAEKVALELRGIRYDDVKGETMGLCFMGDPQKYAF
                     FAKSKA"
     gene            complement(25829..26332)
                     /locus_tag="PK634_03870"
     CDS             complement(25829..26332)
                     /locus_tag="PK634_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOW96903.1"
                     /translation="MKKITFTLNGESRTVAVAPHEVLLDVLRDKLGVKSPKVGCERGD
                     CGACTILLDGKPVRSCLILAVEVEGQEITTLEGVGRDGPTPLQESFIRHNAFQCGFCA
                     PGIVLAATALLEKNPKPTAHEVQEAISGNLCRCTGYEAIIESAVEAGRQGTARARKPA
                     ARKSANR"
     gene            complement(26329..27234)
                     /locus_tag="PK634_03875"
     CDS             complement(26329..27234)
                     /locus_tag="PK634_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOW96904.1"
                     /translation="MSIACEFDYRKPKTLSKAIKLLAKRGRDARVLAGGTDLVGWLRD
                     SLVAPDLLVDIKGFEELKGIGFKNRALVLGALATFSDVLESELVAQHFPLLREMAGMV
                     ASNGVRNRATVVGNICSAVPCCDAGPVLQVYNAVVHVRGPNGKRRVPIGQWFLGPRKT
                     AIRPGEIVTSVTVPLPAKKAAGCFVKLKRYRGEDLAQASVAVLAFSGREYRLAFGAVA
                     PIAVRGPRTEALLKGQKLSAGLLEEAGKLAVEEIVPITDIRSTKEYRSLMVEVMVRRA
                     LKTAAARLAGRGPAYGINVMEEDTP"
     gene            complement(27347..28165)
                     /locus_tag="PK634_03880"
     CDS             complement(27347..28165)
                     /locus_tag="PK634_03880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOW96905.1"
                     /translation="MNTDAFFKLMNNARVYDLTQRLSIHTPPWPSYMPLGIQYFKRIA
                     GAHMGQGANGQIITMSNHVGTHMDGEIHFHASGRSIGAVPIQEWCGPAAVVDISKDVG
                     DYDLYSPELLMKRADIRKGDILIINTGYHRYAWDQPQSDELRYFVCHPGPGPGFAEWC
                     LKMKIKWIGVDCGSADHPMNTIIRTWHPARFAEAEQKLKDTYGKAWDEMFPPKKYYQI
                     MHLKLFPKKLVHAENLGGEIDKVSNQRVWINCYPIRGIEMESAQCRIIALAPPK"
     gene            28330..28791
                     /locus_tag="PK634_03885"
     CDS             28330..28791
                     /locus_tag="PK634_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96906.1"
                     /translation="MKIQLLILASVILLWSAARARPEPPADVQLVQIGRGFATTAERR
                     PGDAVRIRVKEKGDGMKDAIVLSRFTPAQVKLTRGSEAGVELKRWPKAEVEAGQVLVY
                     TLEEAAHIGVELQGSTNSRMEAVRRGDSFELTFTSITERWVLEVKFTSPEF"
     gene            complement(28930..30330)
                     /locus_tag="PK634_03890"
     CDS             complement(28930..30330)
                     /locus_tag="PK634_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOW96907.1"
                     /translation="MKINDLFRSDLKVVNLGLASFKEALDSVGVPAVQVDWKPPVDVE
                     PALLRAVRAHRARIEEANKKVTAKILAAQPVLVGLEKALDVIPGMKPNLILHSGPPIT
                     WDRMCGPMRGGIMCALMYEGRAKSPAEAAQLAASGAIEYAPCHEHHAVGPMAGIVSAS
                     MPVFLVKNEEHGNTAYCTLNEGLGKVLRYGAYGEDVIKRLKWMEEVLYPALKKAVAKL
                     GRIDLKNLIAQALHMGDEVHNRNRAATSLLYRALAPAIVTTGRDPQTTADVLEFINKN
                     DHFFLNLSMPASKAGLDAGRNVPGSSVAVVMARNGTDFGVQLAGTGDAWFTGPADVPD
                     ALYFPGYTKADANPDIGDSAITETNGLGGFAIAAAPAIVQFVGGTANDALNYTRQMYE
                     ITAAENNVYQIPSLNFRGTPTGIDVVKVVEKNILPFIDTGVAHKEAGVGQVGAGVLNA
                     PLEPFKKAFEGFARTL"
     gene            complement(30349..30759)
                     /locus_tag="PK634_03895"
     CDS             complement(30349..30759)
                     /locus_tag="PK634_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOW96908.1"
                     /translation="MKTTILFKDESYRIIGACFEVYKEKGNGFLEAVFQECLALELAD
                     QEIPFVEQPRLGLEYKGRKLKQVYEPDFACFDDIIVEIKAVKQLTDEHRAQAINYLKS
                     TGKQLALLVNFGHFPKIEHERFVHQPLSRVSRVS"
     gene            complement(30803..32326)
                     /gene="fdrA"
                     /locus_tag="PK634_03900"
     CDS             complement(30803..32326)
                     /gene="fdrA"
                     /locus_tag="PK634_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HOW96909.1"
                     /translation="MMVRGKIKKGSYYDSITLMRVGKAVAALDGVTDAAVVMGTQTNK
                     GILGASGLYLSDFDASGDTDLLIAVKAEAEAAVEKAMAAVEKELKEATKKKSAGGEAV
                     RPSSLEGAVEALPGANLAIISVAGRYAGDVAQEALERGLHVMLFSDNVSLETEVALKK
                     FGRDHGLLVMGPDCGTAIINGAPLAFANIVPRGPIGVVAAAGTGLQEVTCLIANEGGG
                     ISQALGTGGRDVKKDVGGIMFIEGLKALAADEKTKAILLVSKPPHPDVLKAIETAVKG
                     IKKPVVSMFLGDETSGGPHTLEEAALTVLARAKGESYEEARRQVESRDLEMKNAAKKL
                     AARLKGRKRVRGLFSGGTFCAEAQVIFRKLGLSGVFSNAPTAGAAKLKDSLKSEGHAM
                     VDYGEDEFTVGRPHPMIDFSLRNKRIEAEAADPETAVILLDVVLGFGAHMDPASELAP
                     VVREAAKKVAVVCSVTGTDRDPQNRPHVIEALEQAGAIVLPSNAAACFMAGFIVATL"
     gene            complement(32323..33147)
                     /locus_tag="PK634_03905"
     CDS             complement(32323..33147)
                     /locus_tag="PK634_03905"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOW96910.1"
                     /translation="MKVVSAGDRVEAGAYRLHSRFRRAANFTSPTRLVVLSDRSVGSG
                     PISIVVDGELPKDDSLTVEPDALLLGGRRYSLGPRYDSALPGIPPEPAMVEVLVNRVR
                     AAAHPKSLAFLLDASRIRNFGDGFEQNMIRQIQAGASKLFGEDPTPGVRLLRGCGFGL
                     TPAGDDLLAGALIGWRLREGRVPPRPWTAWKPSLPGGNVLSESFLALAAEGRVNEPMK
                     NLLTALGGAEAETVRAAANRVLAHGETSGADILTGLVLAVHKGSNFQQPTPNRRGP"
     gene            complement(33144..33491)
                     /locus_tag="PK634_03910"
     CDS             complement(33144..33491)
                     /locus_tag="PK634_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96911.1"
                     /translation="MELQVYGYKCRKCGQLHYPFRMVCKKCKANEPHGFDPVPLPKKG
                     KLLTYTDLSTLPADFNVAQLTLGIVELENGIRVTGQVRIPKPKTGMAVRINIETVRSS
                     EFGKNLGMVFTAA"
     gene            complement(33495..34670)
                     /locus_tag="PK634_03915"
     CDS             complement(33495..34670)
                     /locus_tag="PK634_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOW96912.1"
                     /translation="MRNVYVVGVGITSFSRLEFPISEIAAYPAMMALQDAGNPKIDHL
                     YVSNMGGGRVNGQTALASAVVDTLSLTPIGAETIENGPASGSSAIKTGFMAVASGLCD
                     TVMVAGAERMREVNNLEATDFVATLTHPLAEYIYGVTLPSLAGMFTRLYMEKYGVTSR
                     HLAMVAVKNHANAMLNPFAHLHQPITIEGILDSPEALTNNPIVADPLRFFDMCPVSDG
                     GACVILACEEVARKVKRPLVRLAGVGQATDTHCVHERENPTDLLAVRGAAAQAMKMAG
                     IKPSDVSVAELHDAFTILEIAESEEVGFFKKGEGHLALERGETALGGKIPINPSGGLK
                     GKGHPVGATGIGQAHEIVTQLRGEAKKRQVKNAKIGLTTNFGGFGNNVVSLVFKRED"
     gene            complement(34673..35440)
                     /locus_tag="PK634_03920"
     CDS             complement(34673..35440)
                     /locus_tag="PK634_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96913.1"
                     /translation="MAVELEKILRPVDAPLPPFPPKFMKVASGEDMVVRQVSREEIPD
                     LLPHVEPLIHVERDYYDIVAVRVYAELLGYYRHRVQDEYVLIAQINGELAAIVNGRSL
                     NKDVGVSYHTLALRRGLRVGAHAFAAKMEYHMDILGHKEVLIVAESPIGFRRWMIEYH
                     LEKRFNVAHELGGVPSWALTKPLFDAARDTLVVGRRPVPEALLKKAQAAILPPSNPPK
                     PPADLLGATRSMNDPTASALLLSRQRLAKLGEPEGKV"
     gene            35594..37399
                     /locus_tag="PK634_03925"
     CDS             35594..37399
                     /locus_tag="PK634_03925"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOW96914.1"
                     /translation="MSGREWMFMVFQVIGGMSLFIFGMHVMTRGLRAAAGEALRVILA
                     RTGRSRVQGLVLGTLVGFLAHSGAATTMVAGFVNAGLMGLAESVPPMLGANVGTALSM
                     QLISFHIGAYCWMAIGIGFILSMVAPGERARESGNALLGFGLLFLGMTTISEAIAPHK
                     DMLAPWLAHIHGETWTGRLIGVAISAALTAAVTSSGAIIGLCFALIGAGVFTQFDQVF
                     PIVLGSHIGTCIVALTASAAMNVEARRSALGHLVFNLFNVALALLAYPLVRAAVLWSA
                     DGLTRQAANLHLFVMTAVSLIVLPASKPVAGLLRRLWPSSEPPPEPSHLDVELLEKPE
                     QALCASIRELRRMAALCVRCMDTNGVVMLKGCRTSLRRLYADEEVINEVKKAMYRYLG
                     HLTDRYLSRRQTLFLQHVGRCMKDLERIGDHLTAIADISIERWKHPEAMVPEPMFRVW
                     FDLFCAAKEVLVMTEQSLNPDLDDFQGRALSILEARDRYVIMSMDAKAEFAGAAEAKT
                     ITPLGGYYLSRYMADLDRLVRHAKSVAFAERQPDFRVKRTKLDREAKPAGAYEPPTPV
                     KAEDYLARLQRMDWLSDSEPALPEEKRPAPGGGPG"
     gene            complement(37401..38453)
                     /locus_tag="PK634_03930"
     CDS             complement(37401..38453)
                     /locus_tag="PK634_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOW96915.1"
                     /translation="MRLRLRSRVFLYLLALLFFFIAAQSVIYALVEYVGWLQNPAEPL
                     AEGFEEVLRATILDLLLLLPLVLVAWWISGRVIAPVRTIAATADRIRAGRFSERIETA
                     LMPDDETYHLADTINAAFNRYEAAVQRLRRFSGDASHQLRTPIAAIRSLGEVALTQPR
                     DTAQYRETVETMLSELERLTRIVDQLLQLSRLESGALRAQFAPVDLAEVIEKVKSIYL
                     PLCQERGLSLEVRAKPGLLVTGSEELLVELLGNLLDNAIRHAAPGGTVRLSAERRGDS
                     AALAVRDDGPGIPTEFAERIFERFASVPGGRPGRAGLGLALAADIAAAHDGQLALVNP
                     GAPGALFECVLPRRRD"
     gene            complement(38450..39172)
                     /locus_tag="PK634_03935"
     CDS             complement(38450..39172)
                     /locus_tag="PK634_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW96916.1"
                     /translation="MERDKSSADPAPSGLLVIEDDPRVSESLARGLGEAGFEVRTVRL
                     LAEATAAMAGLPPALLILDLSLPDGDGVEWLRQLRRNGHRQPVIILTARDAIADRVIG
                     LEEGADDYLAKPFSFSELLARVRARLRTAARESAADVARVSDLEVDRRHREVRRAGRL
                     VELTAREFDVLAYLAQWAGHPVSRDMLARDIWKVNRRLTPLDNVIDVHLSHLRDKIDR
                     GREEKLIRTVRGVGFMLAGDAP"
     gene            39243..40271
                     /locus_tag="PK634_03940"
     CDS             39243..40271
                     /locus_tag="PK634_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96917.1"
                     /translation="MSTFFHTWEKLFETGRRLRDALREQQECIREVYARLAPYLATPA
                     DTPAGYAVRPGAIPGSFYGWRKNIFSTLFHSVYHLLGVPAPRRMLYGKMIHLFRVWVT
                     SADNLLDDEDKVVVPLEMPGSSRVMRQVVALMAADRVLAELLAEAAASGLIEAEQGAL
                     LARESLRRLLPSAAQEATEEGGIGERPPPEHVLGVIHRLKTGLLFNVAFLGPDLLEPD
                     LAAGRRQDLKEALMQFGLGCQLLDDVRDLARDLIEGRHNYVLSVLAHTEPAALADLRA
                     AARDVSDRLYLRVPHAALPAVRRGLGLMRGGLRTLGEAGLGFGGAEADSMARSMLAVL
                     DLEGLPHA"
     gene            40264..40968
                     /locus_tag="PK634_03945"
     CDS             40264..40968
                     /locus_tag="PK634_03945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW96918.1"
                     /translation="MRKKKQHPRSACARRTAGVTLDHAAPWYDWLAPLMTLGLEARLQ
                     RRVVAGLRTDRPMAALDVGCGTGTLTRQLHAAIPAGPGRRVVGLDAAEAMIAVARRKA
                     AGREGLEFQAALAEELPHADGTFDRAISTFFFHHLNYDLKRRALAELWRILRPGGQAA
                     ILDVDIPYSWFGRLCAYSGYWLFRQPEIKENITGRLREALDAGPFRGHWRIASRHSGY
                     LSLFELKKPETKKETT"
     gene            40965..>41450
                     /locus_tag="PK634_03950"
     CDS             40965..>41450
                     /locus_tag="PK634_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96919.1"
                     /translation="MKRVLMILAAAILAGGMAGASTPDDPIGAAVEKYFAGTFAGLKA
                     VADQKPTEDTLRRVMKPLAEKTDGFFGGTLIDTDFVIREVYFKRDFLARGFDLKKVEQ
                     LDYFWELMRKAPAPQLSEPGHGNIMQPRLIAMRYPVITDGRLESVVSIMVRTGAFLEA
                     TG"
BASE COUNT         6680 a        13686 c        14072 g         7012 t
ORIGIN      
        1 caacggggcc ctgggctcgg tggcggcggg aacgggcgtg agcagcggcg cggtcctgcg
       61 attgcagggt agcgtgttct acacggcttc cgaaccgttg gacctggcgg ggaccgtggc
      121 gggatacacg ggcagcaaca cctgggccgg accgatatcg ctggcgggcc cggccgtctt
      181 cgcggcgagt tcgtttgcga cgttacacct gtccggctcg ataggcgggg cggaaggcgt
      241 ggcgatctcc ggaggcggcg tggtctccat cggcggggcg gggagcagca gctacgctgg
      301 caccaccacc gtccattcgg ggaccttgaa tctcgggcgc agcgccggac cggccgtccc
      361 gggcgccctg gtggtcggcg gggaggaggc ggcgaccgtg cgggtggggt ggatgaacca
      421 gttggcggcg acgaatcccg tccgggtgca ggaatacggc gtcctggacc tgaacagttt
      481 cgggctgttc aacccgctcg ggcaactccg ttggcggggc ggttccgtca gctcgggcaa
      541 cggccccatg aatctggtca ccgacgtggt cgcggaagcc tccgccttat cggcgggcct
      601 ggcgggcatc gtgagcctgg gcgcgaccga ccgggtgttc ctagtggaaa acggcgcggc
      661 ggagaacgac atggtggtct ccgcgatgct ggggggaggc gggatccgca agacgggggg
      721 aggccgcctt tggctgggtg gcggcacgtt cgaatctccc gttcgggtgg cggagggaga
      781 ggtcgttgcc ggagcggacc aggccctggg gctggggccg gtgttcctgg gcgagggaag
      841 cgccttgggc gtggggctgt actttggcgg gaacggccgg ttgaccaacc gcctggaggt
      901 gacagccggc gcgcggattt tgggcacgct gcccggcgtg gcgacgggca cgtgggcggg
      961 gcccatggtc gtggaggggg cgttgacact tcaagctgcc gcagcgagcc gccttgtaat
     1021 cgagcggggc ttggagggtg ctgcgggggc ggtgacggta acaggcggcg gcgtggtgga
     1081 actgcatggc gccgggacct atcccggcgg gacggtcgtg gcgggtggcg ccatgtgggc
     1141 gtgtcacgga gggagttcgg ccgtcggagc gggggcggtg caggtgcaag ccggcggcgc
     1201 gctgggtggg acgggcgtcg tccatggccc cgtgacgctg gccccgggcg ccactttgaa
     1261 tcccggttgc tcggccggca cgttggccgt cagcaacctg gtttccctcg aagacggcag
     1321 ccggttcgtg gtggaggcgg aagcgctggc gtacgacgcc ctggacctcc gccgcggtgg
     1381 cgcggtcgcc ctggcgccgg ccgcgcaact gctcgtgccg ggcccgctgc cggggacaga
     1441 cgcctgcgtc atcgttcgcg gagcaacgca ggtcaacggg atcttttcgg ggctgccttc
     1501 cgggaccgcc ctgcccccgc ccaacgagga ttggtatatt cattacaagc cgacgggcat
     1561 ctacctcggc cggaacgccg caccgctctg gtatttccgc ggctatgcca acgaaggggc
     1621 ggcgatgttg agctggcgga cgccggtcga gctgggcgtg ctgggctacg acctctatcg
     1681 cgacgagggc gggtcgtggg tgaaggtcaa cggcgacccg atcccgccgc gggacccgaa
     1741 tggcgcggcg tacactttcg aggatccgct gatggggccg ggcatgaccg ggcgttaccg
     1801 gctggtcgag ctgtacgatg ggggattgtc gaacgaactg gtggactgtg cgcgcgagtt
     1861 cacggagctg gccttcacgg cgggcccgag ggagaccgat ggcgggctgg agcttcgctg
     1921 gcggagccgg gaggatgagg cgtactggct ggagcggtca tccgaccctg cgcaggacta
     1981 tgtcccggtg gccggccccc tgccggccac gccgggggaa aacacgtaca ccaatcccgt
     2041 ctccgacacg cgcggcttct atcgcgcggt gctcatgccc tagcggcgcc cgcgccgcgg
     2101 ggggcgccgt tttcatcaag ccttgttccg gtccagcgcg tcgcggacag cctgcgcgag
     2161 gccgcgctgg gtgaagggct tggtcaggaa ctgggcgccc gtgatgcgga tcaggcgctc
     2221 ggcggtcttc ggcgtgaacc cggacacgag cagcacgggg atgcccggct tgatccggtg
     2281 gatctcccgg tacacgtcct cgccgttgac gtagggcatc acgacgtcga gcacgaccag
     2341 gtcgatctcc gcgtggcgct gcctgaaata ctcgatggcc tcctcgccgc ccggcgcgcc
     2401 gtggacggaa tagccggcgc gttggagaat gcgctgggcg atgctcaagg ccagcggatc
     2461 gtcgtccacc gccaggatgc gctcggtccc tccgggaccc gggccttcgt ccggccccgg
     2521 gcccgatacg gcggccgacg gggtgtcggc ggtgggcagg tgcagctcga agcacgcgcc
     2581 caggcccggc tccgagtgga gcgtcaccca tccgcccatc gcgcgcacgg catggcgcac
     2641 cacggtcagg cccatccccg tcgcggcctt gcgcgtcgtg aagaacggtt caaacacccg
     2701 gtcctggatc tcgggcggga tgcccggtcc cgtgtctgac accgagatca ccaggtaatg
     2761 ccccggccgc tgctcgggcc gggactggat gtcctggtcc gacagccggg cctccgccag
     2821 cccgagggtc agtttcccgc cttccggcat cgcgtctcgt gcgttcaggc acaggttcag
     2881 cagcgcccgg tccagcgtgg cccggtccgc gcggacgcgg ggcagcgtct cccgttcggg
     2941 catggacagg gtgatgttgc ggggcagggc ctttttctgg agcgccacga tgtcgcccag
     3001 gacgtcgtgg atgtccacgt cctccagcac gggcgtcgag tgccgggcga cggcgagcag
     3061 ccgcgcggtc aggtcgttgg cggcctgggc ggcctgctgg atgcgcgcca cgtcggcctg
     3121 gaggtcggag ctgggcatct ggtcccgcag cagcgaggcg taggccatga tgatggtcag
     3181 gaggttcttg aactcgtggg cgatgccgcc ggacaggtgc tcgaccagtt ccatctgctg
     3241 cgcttgcatc acctgcttca ccagcagccg ctcccgcgcc acgtcgcggg cgacgaacac
     3301 gtaccccagc gtccgttcct tcgcgtccag caatcgttcc gcccggatgt ccagcagccg
     3361 ttcggcgccc cggggaggat ggcccgtggc ctcgccgctc caccggccct cgcgctcgag
     3421 ggcgttcagc acgctgtcga aggacgggcc gccgtccggt ttcagaaggg cggacaactc
     3481 gccgagcgtg gccggcgcgc ggcccagcag gccggcgatc gaaggagtgg agaaaacaac
     3541 ccggcccccg gcatccgcca cgagcaggcc gtcgctgata taatccagcg cttcaaggcg
     3601 ctctggactt gattcaaagg tcatggtgca gcgagcgcgt gcaccagtaa acccggcggc
     3661 tggagaaaag tcaatgggcc gccgaggcgg aggccgatgc gcgcttgcga aggagcagcg
     3721 gtttgccgta ttttcacggg cgagcggcga acgccgccgg atcggaggcc cagatgtgcg
     3781 gcatcgtggg atatgtcggt cgccgtgacg ccgtcccgct gctggtggac ggcctgaagc
     3841 gcctcgagta ccggggctac gactccgccg gcatcgcggt gatggagaag ggccggctcg
     3901 agatccgcaa gtgcgtgggc cggctgtccg cgctcgagga gaagctggcc cgccagcccg
     3961 tcgccggcgc ccccggcatc ggccacacgc gctgggccac gcacggcgag ccctccgaga
     4021 tcaactcgca cccgcacaca gactcgaccg gccgcatcgc ggtcgtccac aacggcatca
     4081 tcgagaattt cgagtcgctg cgccatgacc ttgaaaagaa ggggcacccg ttccgatccg
     4141 agacggacac cgaggtcgtc ccgcacctga tcgagcagga gctgaaggac ggcgcccggg
     4201 acctgcccga ggcgatccgc cgcgccctgg ccaaggcgcg cggcgcgtac gcgctgggca
     4261 tcgtctcgct cgacgacccg gacacgatct acgcggcccg cctgggcagc ccgctgatcg
     4321 tcggcgtcgg cgacgacggg cagttcatcg ccagcgacgt gcccgcgatc ctcaaccaga
     4381 cccgcaaggt catctacctc gacgacggcg agctcgccat cctgaaggcc ggcggcgtgc
     4441 gcgtgatgaa gctcgacggc accgaggtca agccggacat ccgcgaggtc aagctccagg
     4501 ccgaggccgc cgagcgggcc ggcttcgaga cgttcatgcg caaggagatc cacgagcagc
     4561 cgcgcgtcct gcgctcgttg ctggacaagc acacgaagga cgccgccgcg gtcgccctcc
     4621 aggacgtcgg attcgacgac gcccagtgga aggccgtccg gcgcatcatg atcatcagtt
     4681 gcggcacggc ctactacgcc ggcagctacg gtcggctgat catcgaggcg ctgaccggcc
     4741 tgccggtgga gatcgagttg ggcagcgagt tccgctaccg ggacgccaag ctctcgcccg
     4801 acatgctcgt cgtgtccgtg tcgcagtccg gcgagacggc cgacacgctg gcctcgatcc
     4861 gcaaggcccg ccgggcgggc tgcaaggcgc tggccatctg caacacgccg ggcagcagcc
     4921 tggcgcggga aagcgacgtc gtctttcaca cggaggcggg ccccgagatc ggcgtggcct
     4981 cgaccaaggc ctacacggcc cagctcatgg cgtttgccct gctggccatc cacctcggcc
     5041 gcctgcgcgg ggaactgacg gcggagcagg agcgggaact cgtgcgcgac ctgcacgccg
     5101 tgcccgacgc gatccactac gtcctcgcgc gcggcgagga aatccgcgcg atcgcccaga
     5161 aacacggcga cgggcccagt gccctgtacc ttggccggaa gttcaactac ccgacggccc
     5221 tcgaaggcgc cctgaagaac aaggaaatct cttaccagca cgccgagggc tacgcggcgg
     5281 gcgagatgaa gcatgggccc attgcgctga tcaacgaata cctgcccgtc gtctgcctct
     5341 gcacgaagac ccgtgacgag gtctacgaga agatgatctc caacatgaag gaggtcgagg
     5401 cccggcacgg ccggatcatc gccgtcgcca gcaacggcga cgagagcgtt ctgaagctcg
     5461 ccgaggacat catcttcgtg ccgcaggtcc gggacgaatt ctcgccgatg gtcaacgtcg
     5521 tggcgctgca actgctcgcc tacttcgccg cgcaggcgcg cggcaccgat atcgacaagc
     5581 cccgcaacct cgccaagagc gtgacggtcg agtagcgcat ccagcttgta tgcagcgagt
     5641 tgtgcctaca taggcatgtt cttacaagcc ggccgaaaag gcttgtgcgc cgttggggcc
     5701 ccggctatcc tgccttcatt cagcaggggg gggcggtttc acgaggagcg aatgtgccga
     5761 ggcacataag agcgtggagg cgttccagct cgttcacgcc gcggttgatt tcggtccggc
     5821 cttttttatg cgcagcgatt ctctttctgg ccctcacctc ccgcgcccag tggcggaccc
     5881 agtccgttga tttgaatccc ggctggaatg ccgtctacct ggaagtccag cccgacccgc
     5941 ggctgctcga cgaggtgttc gccggccgcg cggtggaggg cgtgtggaag tgggaccgtc
     6001 ggttcagcca tatcgagtac gtgacggacc cgtgcgtcct ggagcccgag gacccgcact
     6061 ggctggtctg gctgccgcca gaggattcgc gccgatttct ctcgcgcctg ttcgagttgc
     6121 agggcgggca ggcttacctg gtcaagatcc cgaccaacgc ggcgggctac tcgctgccgg
     6181 tcaaggggcg cgtgatcatc cccgtgacgg attggttcgc ccacggcctg aacctggtgg
     6241 gctttccggt gcacacaacg tatccgccga cgttcgcgga tttcttcagg ttcacggacg
     6301 agattgacac gacccgggga gtgaacaacc agcttttcag catcaacagc gcggggcggg
     6361 gcatgacgat cgtgcagccg aaccgggaga ccatcgcggc gggccggtcg tactgggtgg
     6421 gctgcgacag cgatccgaaa tacccgggac cgctggtcgt cgagccgtcg agggtagggg
     6481 cggtggactt cgggtcgcac gtggtccagc aggagttgac gatccggaac ctgggtccga
     6541 ccgccatgac ggtgcgcgtg acgcaggagg cgtcggagga ggaatcggag ggcttcccgg
     6601 aactggcggg ggcggtgccg ctctcttaca tggtccgcaa tgcgagcagt caatgggagt
     6661 ggatcgagtt cccggtgggc ggcctgtcgg aggtcgtggc ggcgggcggg gcctggacga
     6721 tctacctggg tgtgcgtcgg caggatttcg tggcgtacgc gccggtgggg acgaacgggg
     6781 cgctgtacca gagcatcctg aatgtggagg acacggccct atcgttgcgg gtccgggtgc
     6841 cggtgatcgc cgagaagccg gcggggctga tgggggcgga gttaagcgac catcacgaga
     6901 gcgagggcct gtgggtgggc caggcgacgc tcgaccgggt caatgcgccg gcctatacga
     6961 acggattgct gcccactccg gcgccgctga ccgtgcggct gatcgtgcac gtggacgcct
     7021 acggccgctc gcgcctactc cagcaagcgg tcctggtgtg ggacggcagc ctgaccaacg
     7081 cgccgcacac gaatgggacg tatgcgttgt tcgtgaacga cgccgaaatg ccggcggatg
     7141 ccgaggaggc ctatcggatc agttcgaccg ccttcccgct catgccgccc ctcctgttgg
     7201 acggcgactt caccaacgtg ttgaccggca cggtgacggt ggactttgac gacccgacca
     7261 atcccttcct gcaccggtat cacccgatgc acgacaacaa gaactgggac ttcgaatcgt
     7321 acacgaacgc cgtcgagacc cgcacgatcg cgcgggacgt cacgctggat ttcgaggagt
     7381 ctccgaccaa cctcgtgcat cacccgtact ggggcgtaga ccagatcctc gggacctatc
     7441 gcgagacgat gacgggcctg cgggcgcagc cgatccatgt ggagggggat ttctggatgg
     7501 agcggattag tacgatcaat gaactgcaat gaaacgatga ccatgaaggt agggcgcgtt
     7561 ggctcaacgc gccgaacagc ggcggcttga gccaagccgc cctacctatc tgaatttaaa
     7621 aagcaggaaa aaggaagtga gcatgaaaac acgtctgaat gcccaatggc tgttggtgat
     7681 ccttctttat tcatccttca tccttcatcc ttccgcgttc tccgccgacg tgcccccgat
     7741 catcaactat cagggtctgc tgacggacaa cctgggcaac gcggtcaccg cgggctatta
     7801 tgagattgag ttccgcgtct gggacagccc gaacctgacg ggcgcggggc acctgatctg
     7861 gggccggtca ttccctctgc acgtgatgag caacgggacg ttcaacatcc tcttgagcaa
     7921 tgacggcggg gcgatcacga acccggtgtc gccggtgacc aacgacctcc gcgggtcctt
     7981 cgagagcacg gaccgttacc tgggcctgac catcacgcac aacccgctgg gcgcggtggg
     8041 ttcgccggcg gagatcagcc cgcgccagcg gctggtcagc gcgccgtttg cctttcacgc
     8101 gcagcacgcg acggagacgc cggggacgat cccgatcggg ggcatcattg cctggtccgg
     8161 cgccgtcacc aacgtcccgg acagttgggc gttgtgcgat ggcggcacct attcaggcca
     8221 ggtcacgccg gacctgcgca accggttcat cctgggggcg ggcggcgtgt acgccagggg
     8281 ggacgcgggc ggcacgaacc aggtgacgct gacgatagac gagatccctt cgcattcgca
     8341 cggcggcatc cgcgacaagg gggaaaacag cgcttaccat gccacggaaa ccggcggcta
     8401 tttctggaag catgacaatt ggaagaacac tgacaacacc ggcggcggcc atccccacga
     8461 gaacatgccg ccgtattatg cgctgtgcta catcatgcgg gtgaggtgaa ggaaaggatg
     8521 aaggcggaag gttgaaagcg gaaggcggaa ggatgaaggc ggaacgaaga aaagcgcgct
     8581 tttcttcacg gttcatcctt catccttctc ttcgcatcct tcatcgttca tccttcatcc
     8641 ttctcttcgc ctccttcctc cttcatccct catccttctc tttcgcttcc ggctacaacc
     8701 tcgacacgct ctaccagaac cgctggttcg aaccgagccc cgcgggcggg ccgtatacca
     8761 attatttcgg cgcgtggccg gggggaaccg tctcgaacgc ctcgacggcg atctataacg
     8821 ggcacgccat gggcaacacc aacgccgtgt cgccctccaa cgcttcctac cgcctggctt
     8881 cgtccgcgtt cgggcagccc gtggagggga tcaaggcgga cgtcgcgctg ggcgacgtga
     8941 ttccttcgcc gccgggggcg aacacgaacg tgccgccggc caatttcgtc gccgtccagg
     9001 tcggcggcaa cagcgcggcc tactatgaga gcacggaccc gccccacgcc gcgttctggt
     9061 cgccctcgac caagcaggtc atcgcggccc agccggacag cgtggccgtc gcatgggtca
     9121 tgaccaatca cacgacaaat gtcgtcgcga cctacgtggg cgcggtgccg tccagccgac
     9181 ccgcccgcat tttctggacg gagagcccgt acgacgcgcc aaggatcaat ctgaacgggc
     9241 tcttcccggt gatccattac agcaagaccg tgccggcccc ggtataccac gtggtcacca
     9301 acgtcaacgg cggcgtgacg aacatcacct ccaacgtcgt cagcggcgtc tggatagacg
     9361 gccagaagca gatgcacgcc aaggacacgt cggggatgtt catcctcgag tattaccagg
     9421 cgggcacgta caaggacgaa gtccagccgg acggcatcga ggtggtgcag gtgctcgagc
     9481 cggacctcca ggtgatctgg gccgacatcg gcagccgcct gctgcccgcc gactcgtact
     9541 gggcccaggt ggacggcaac ggcgggatca ttccgctggt ccaggtcgga ttgaacgaga
     9601 cggcctacat ggtggaccag gtcgggccga aggacaactg ggtgttctcc atcaagaaga
     9661 ccgtgtacga gccctggtcg ctggagatct actggcagca ccgggggctt atgggcgtgt
     9721 tgtggcccta cgagaaggac tggtactcgt gcgactggcc gctgcacccg cagctttatg
     9781 tcttcggctc gtccacccag gacgccgccg cggtgctcat cccggacggc ctgaccgcgg
     9841 cgctgatgga gtacatggac ccggccttcc acgcggagat ttcaaccagc gggcgctcct
     9901 tcagcgccag cgagccgggc cggtgcctgc tgaagtacac gacgcacgac gacatctggt
     9961 tcgaggtggt ccagactgtt tcgcacacca actcgaccta cttcgatctg gacccgaacg
    10021 actggacgat cggcgacgaa ctgacccccg gccccgagga ggcccacgcg ctggccttcg
    10081 acggcacgga cgactatgtc agcctgggca cgagctgggt caacgggcgg agcgactgga
    10141 cgctggccct gtggttcaat cccggcgccg ccgcgcccgg cgtcctgtat tccgagggcg
    10201 ggtccggggg cgtcacgttc gacgtcgtcc tccaggccaa ccgcgccctc tcggtggggt
    10261 tctggaacaa gtatcacctc actaaccact gggtgagcct cgttacgccc acgaatacgg
    10321 tgcgatcgaa cgcgtggcag tatctggccg tgaccttatc gggcgcggat gattccaacg
    10381 ggacggcgcg cctctacctc gacgaccggt cctgggaggc cacgaatgtc tggatcgtca
    10441 acttcgacgg cagtcaactc ggcctcatgg ccgcctcgcc gacctcgcat ggcatcacca
    10501 attttttccg cggtcggctg gacgaggtac ggatctggag cgaggcgttg tccaccgggg
    10561 ccatccggag caaccagtac gacacgtctg acggatactc ggggacgcag ttggcgaact
    10621 actccttcga cgagggcgaa ggggcggtca tccacaactg ggcgggcggc tatcatggca
    10681 cggccgtcaa cggtccgctc tggtgctacg gacaggtcac gcctggggcg gactacgcca
    10741 gtttcccggg ctacgttcat gtcccggagg gggaccgcta caacgtccat cgctacgact
    10801 atcccaccga ggccaacccg ggcgccgagt cctatgtctt cgcggtcaac acgaatttcc
    10861 tcgaggtttg gtgggccaac cggagccggc aggacggcat gccggcgctg tactacccgt
    10921 cgcacgtcac gcgctaccgc aacctgtggc cgacgaatgc cccgcacatc gtgattgcca
    10981 gcggtttggg gagccagggc gaccagctcg tgcccgcgac caacgcgctg gatttcaacg
    11041 gcacgtcgga ctacgcgcgg gcgccgacca atcccccggc cctgggcatg gaaggggcgg
    11101 tcacggtcga ggcgtgggtg caactggatg ccgccggcga tcggaagatc gtggccaaga
    11161 cggattcggg gccgctctgg aagggcttgg tcctgggcgt gaagagcaac gcctgcttcc
    11221 ccgagctctg ggacgccgcc agcaatcact actcctgcct ggccggttcc gtgccgacgg
    11281 gagcctggac gcacatcgcc ttcacgtacg agaccgggca gtacttccgg gcgtatgtca
    11341 acggcgactt gatgcaccag gagacggcgg gcgggcccat cagcgggtcc acgtcgaccg
    11401 tattcattgg gcgttcgtcg tgggaaacgc cgtattacgt ggacggccgg atcggcgagg
    11461 tgcggatctg gagcgtcgcc cggtcggcgt cggacatccg cggggcgatg tacacccggc
    11521 tgcggggcac ggagtcgggc ctggtcttgt actatccttt cgagccgggc gagtcggacg
    11581 ccgtgctcta tgacgaaggg accaacgcgc tggacgcgac gatcagcggc gccgactggg
    11641 tcgccttggg ccggccggtg gctgccgcgg aatccttcgt ctttggctct ccctcgattt
    11701 acagccagaa cacgcccggc cagcccgggt ataatcccaa cgaggaacat gcgatgatgc
    11761 tgggcggggt cgcctacgcc ctgcggaacg acctgaactc cgacctctac tcaaagccct
    11821 tcgtgctggt ggactacctc gatccgaaca ccgcccggcc cgcgatgaag gccttcgagg
    11881 ttgtgcgcag caacacgtat taccgattcg aaaggagtgt cacggccggc ctgccgatcg
    11941 tgccgctcat gcccctggcc gcgctgccgc cgtgcacgaa cacgaccagc gataccatgc
    12001 ccccggcctg gcgcgaccgc aagctggaat ggtgggcgcg gtccgcaggc gacgacggcg
    12061 gacccagcaa cgccatcctg cgtttctatt acccgatcca gccgaccttc tacttcccgg
    12121 gcaaggcgac caacgcgcag cccgcgctgg acgcggagat cccctggctc cccgatccct
    12181 cgagccagaa cggcgcggcg gggacgcccg tgcctgtcac gtacgccgtc acctggccgg
    12241 acgacgtgcc ggaactgaag atcgcccaga cgctgaccca gccgacccac gggctgccgg
    12301 acctctggga ccagttgagc atcgaggtcg tctaccagca gtccctgcag accaacgcgg
    12361 gctcgtgcgt gacgctgttc gatcccgtcg ccgcccaggg cgcggcgctg agcaacgacg
    12421 tggtcgaggc tatgattgag agcggcctgg cgacgcggga cctggtgagc gaccgggtgc
    12481 ggttccccgg gctcacgcct tcgctgtacg gccgggtcta ctacgatccg gatcggggca
    12541 ccaacggccc gctggtcctg gagggacagt acgtgacgcc gctgacgggc gacggctacc
    12601 tcctgctgaa cgtgctggag gattacgaac ggaaccaggc gaagagcgcc gccgtgggcc
    12661 tggagccggc ggacaaggcc gcgtgggacg ccgcggtgga cagcctgccg gtgaacgtca
    12721 tccagatcca gccgaacacg cccttcgtca acggggccct ctacgcgggc ttgagcaatg
    12781 gggcgggcta cgtgaccctg gccttcaaca acagtacgaa cggccaccag gtgccgacgg
    12841 ctctgcccgt cagcctgagc atcctccagg tcgtgcccga gctctacaac ggcatgctcc
    12901 aggtgatcga gcctgaggat ccgcttgacg agcagctctc cctgcgcgcc agcccggact
    12961 tcgcggggtg cctcgaggat tacgaatttc agtggcgctg ggaggaaccc gagggggggc
    13021 tgatccccaa cacgaacttc ccgcgatgga acgcatacgg ggcggacacc gtcgaggcca
    13081 ccaacgagat caccatcgcg ggcgccagct cgttcaccct ggcggatcac tacttcgccc
    13141 tgcggtaccg gcggacgggg ggcagcgggg tgaccggcac gaactggagc gaatggacct
    13201 acagcctggc ccccggctgg atccagcgcg tgttgaacgg gatcaacccg ttcacccagt
    13261 gcctggagga catgatcgag aacgccgtgg acgtccgctc gacgatgatc agcctggccg
    13321 gcccgccgta cgagggcgac attgccctga acctggcggc ggcctgcgag gccgggctca
    13381 tccccgtcta ccgcaccgtg ctcaaccgcg ccgaggactt cagcatccgg gcaggagtca
    13441 gcgaccaggc ggacaacgaa gccctgctct ttgccgcgag ccggctgcac gacctgtaca
    13501 tgctgctggg ccacgaggcc tacgccgacg cccaggaccc gacgatctcc ttcccccgcg
    13561 agctgtacga ggacgagcac ggcgcggagg ccacaggcct tttctgtttc atgaaccagg
    13621 ttccgaacct gctggaggag gagctggcgc tgctgcgcgg ccgcgacgac accctcgagc
    13681 cgctggtcac cattgggccg gtttacaacc ggttgatctg gaacttcacc aaggggatca
    13741 acggcggcga gcccgcctac gcctacaatt acaacatccg cggcacgccg accaacacga
    13801 tcggcgcgat caccgccgag gacgccaagc gcctctaccc gcagggccac ggcgacgcct
    13861 ggggccacta tttgagcgcc ctgtccggct actacgaact gctctcgaac accaattttg
    13921 tctggcagac caagcccggg gcgacgttat tgggcaacgc gactgtcagc agcgatttct
    13981 tcgacgagca gaagttcgcc gaggccgccg cggccaaggc ccggaccggc gtggagatcg
    14041 tcaaccgcgc gtaccgcctg gcctacgagg aggatgcgac cgtccgctgg gcgggctacc
    14101 gggattccaa cacgaaccgc gaatggggcc tcgccggctg ggccagccgc accggccagg
    14161 gcgtgctcta cgattggacc gtggctaact cgctcctgct ggaccacctg acgaatatga
    14221 cccaggtcgg cggcgcgaac gagccgcccg aaggcatcca gaagatcgat cggagcaccg
    14281 tgcccgaatt atgcgagatc gcttcggccg cggacgaggt acagcggcag ctcgaccatg
    14341 ccgactacgg gatgaatccc ctgggcctcg accgggacac cgtgtccttc gacatcagtc
    14401 ccacggagat tgacgagggc caaacgcact tcgagcagat ttacgaccgg gccctccaag
    14461 tcttgtacaa tgcctgcgtg gccttcgact acggccgcaa catcacgctc aagttgcgcg
    14521 agcagtttga ttcggtctac gagcttcagg agcaactggc cgagaacgag agggatttcc
    14581 acaaccagct catcgagatc tacgggtatc cctacccgga cgacgtcggg cccggcggga
    14641 cctatccgca gggctacgcg ggcccggatc tcgtcaactg gcagatcctc gacctggaag
    14701 acctgcggac cgacgcgccg accggccagc cgttccgggt ggacctctac aattacgtgt
    14761 tcaattccgg caacgagttc agcgggacga actactcgga ctactcgaac ctggccgcca
    14821 ccaacaactt caccaccagc gtcgtgggga ccataaccgt ctacatcagc gacagcgggc
    14881 tgaaagtcaa gccgccggcc tggacgagcc gccgccccgc gcagggggag ttgcagatgg
    14941 cgctgtcgga gatggtccag ggctggtacg cgctgcaggc gaagaaggcg gaatacaacc
    15001 agaccctgta cgagctgaag gtggagctgc tgcaccgcca ggccgactac acccggtatc
    15061 ccggcgaatg gtccaacagc gtcgagaacg cggagcgcaa gaaggaaacg gcccgggtcg
    15121 tcgaggggct ggagatcacc tcttccctgt tggatatcgt cgccagctgc ttgagcgacg
    15181 tccagtacat gctcgccggg ctgatccccg accagtacga cggcctgatc ggtccgttcc
    15241 cgcacctcgg cgtgaacaat cactggtcgt ccgcggacaa ggtggccatc gcgctcagtg
    15301 tgtacgtccg gcgcctggcc atcgtggcca tgcaggccgg cgtgaccggc cgcgagaagt
    15361 tgcaggaacg ctgggacgcc gatctcgaga aactgatttc caacaatgag taccaggaca
    15421 tcctgcactg ggcgacgtgg gagacccagg ccaaattgaa ggaacagttc gtcaagcagg
    15481 cggaaatcat ggagcaggtc caggccctga accagcaggt cgaggaggtg cggaagctcc
    15541 tgacccaggg ccagctcctc ctgacccagc gggcccaggt gcgctcgcgg gccgcgcagc
    15601 gcatccagat gaaccgctac caggacatcg cgttccggat cttccgcgac gacgcgttgc
    15661 ggcggtacaa ggactcgttc gaccttgccg cgcgctacgt ctacatggcc gcgaaggctt
    15721 acgactacga gacgggcctg ctggactccg acacgcggct cacgcctggc cgccggttca
    15781 tcgaggatat cgtgcgggcg cggctccccg gccgcttcta cgtctggctg ggcacgcccg
    15841 agaccggcat tgacgagggc gaacccggcc tggccgacat catggcgcgg atgaaagcgg
    15901 attgggaggt ggtcgaaggg cggttcggtt tcaacaatcc cgacacggag accagccgct
    15961 tctccttgcg gaccgagctc ttccgcatct cggcctcgtc caacagcgac gggacctggg
    16021 cgcaggtgct ggaggaccac agggtggatg atctgcgcga gctgcaggag ttcaagcggt
    16081 actgcatccc gttctccgat tcgaccaaca ccgagccggc cctggtcatc ccgttctcga
    16141 ccctcatcgt ggccggcaag aactacttcg gccacaacct cgccggaggc gacaacgcct
    16201 acgacccgac gcgccaggcg acgaagatcc ggtcggccgg catttggttc acggggtaca
    16261 acgtcacgtt caacaccaac accgcgggca atggcctggc caacgagccc cgggtttatc
    16321 tgattccgac gggcgaggac gtcatgcgct cgccgacgcg cgaggggctg gaaacgagga
    16381 actggaaggt gctggaccag gccctgccgc tgccctacga cgtcggcggc atgaacatag
    16441 acaacccgga ttaccagccg atcatagact cgctgaccga accgctggcc cagatccggc
    16501 ggttcgcggc gctgcgcgcg taccacgacc gcggccagtt cgacgaggcg gagacgcaca
    16561 acaacgggcg gctgatcggc cgctcggtct ggaacaccca gtggctgctg atcatcccgg
    16621 gccggaccct gctggccgac ccggaggagg gcatccagcg gttcatccac ggggcgaagg
    16681 tcaacaacgt gcgcgacggc aacgggatca aggacatcaa gatcttcttc cagacgtatt
    16741 ccatcgccgg tgaatgaaga gaacgattaa gcttaacgat aaacgctaaa tggaagaagg
    16801 atgagcgtgg aaaagccgat ggatttgaag ggccgtttga gggactacgc gctgtggata
    16861 atcaggccgt actgttcgct gtccaagaga gatgatgtcg cggtggtttt ggggcggcag
    16921 gtgttaagaa gcgggacatc gccgggtgcg cagcaccggg aggcttgtcg cgccaagtca
    16981 acggccgagt ttatcagcaa gatggaaggt gggttacagg agttggagga gactgaatac
    17041 tggctggact tgctggaagg cagtcagcgt ttgtccggcg atacggtcgc ccccctgatg
    17101 cgcgaaacac gggaactgaa cgccattttc acggcatcca tcaagaccgc caaacgccga
    17161 aagcgtgaac aagatgaaag cgcgtagtct ttctttagcg tttatcattt atcatttatc
    17221 gttctcttcc ttcgccggca tgccgcagcc gatggtggtg tattacggcc aggcgaagga
    17281 cgacttcggg tggccataca cgcgcgacgc gacggtgatc ctgcggagcg ggacgaacga
    17341 tgtcgcgctc cacagcatcg acggctcgct cacgccgggc atcaacttcg cgctgtacgc
    17401 gccgctggac gacgggcggg acacgaaccg ctacgtgcgc aacgcggtgc ggacgggaga
    17461 ggtagtgacg atcgtggtcc gggacgcgtg gggagaacgg acgctgctgg agaccaactc
    17521 catccccgcc gtgacgacgc cgggcgacct gatctgcgtg aacgtcaccg cgggctccga
    17581 ttccgacggg gacggcctgc cggacgagtg ggagcagtgg ctggtggatc tctccacgaa
    17641 cccggcgatc acgtcggtgg cggacatcca tgcggaggac gacttcgacg gagacggcgt
    17701 gccgaacggg gaggagtacc gggcggggac gttcgcgttc ctggattacg actacttctt
    17761 catcgagcgc tcgcggctgt cggcccaggg ctggtggctg atccagttct tgagcgtgcc
    17821 gggcaaggtg taccggctct acgaggccta ccaggtgccg acctatccgg gcgacgcgtg
    17881 gtcgccgggc gtgtattcca ccaccgatgc cggcgcgctg ggggcgggcg tcatcgaggg
    17941 cgacggcggc tggacctcgc tgtatatccc cgtgaccaac tcgccccgcg cctacaagat
    18001 gcacgtggaa tgacgtcctt ctttcacagg gccgcgctcc tgatggccgt gctttccctg
    18061 gccgccggcg ccgcgcatgc cgacctggtg gcgtccgaca acgccgacaa cgacccgtat
    18121 ccgtccggct ggaacccagg agacaacgcc ggctcgggct ttacggcatg ggtcagcctg
    18181 caggccggat cgcccggttc gatgtacgtg gaaaacctgg cgcctctgga cggtttgtat
    18241 tcgtggggct tgagcggcac ctatgccgtc gggcgcggcc tctccaacag cctcgccgcc
    18301 ggccagtgga atatcctggc cagccatggg tcggacgtcg gctcgttctg cggcttcaac
    18361 ctgcgcacct cgacggacga ctccgcgttc gacacggatg aaattctgcg cttcgggatc
    18421 aactacgggg aggaattcga cgcgacgcgc atctacttct ccaccaacgg cggcgcggac
    18481 tatgcgtaca tcgacctggg cgacgccgac ctgcgggatt ccgacctgga gtacaatgtc
    18541 tcgtggtcca ccgtcagcgg ctcgttcacg ctcggggtgc ggaacggcga ggactacgaa
    18601 gaggtgacgg cggatctgcc ctcggatgct gcggtggcca tgttcggggc agggattttc
    18661 gaggccagcc tcgatgagcg catgaccttc gacgattacg gcgtcgaggc cattcccgag
    18721 cccggcgcgc tggtgaccct ggtcctgggc tgcgggttta ttcagtggtt catgtaccgg
    18781 cgcctgcgca gggcggcgcg gttgctcccg ttctagccat tttttgcttc atccttccgc
    18841 cttcatcctt cattcttgtc ccatgtcgcg attcatcatc cacggccagc ggaagctgtc
    18901 cggcgaatac cggccggtgg gcaacaagaa cgccgtgctg ccgatgctgg ccgccgccct
    18961 gctgacggac gagcccgtga cgctccgcaa cgttcccgac atccgcgacg tcgaggtgat
    19021 gctggcgctc ctggaggggc tcggcgtcga ggtggcgcgg gagggcgacc gggtgacgct
    19081 gcgggcgggc tcgatccggc gccggcggct ggaccccgac ctctgccgcc gggtgcgcgc
    19141 gtccatcctc ctggcggcgc ccctcgcggc gcggcacggg agctgtgtct tgcccccgcc
    19201 cggcggcgat atcatcggcc gccggcgcct ggacacgcac ttcaacggcc tgcgcgcgct
    19261 gggcatcgcc gtcgagggcg gggcccacta cacgctgcgc cgccgtcagc tccgcggcgc
    19321 ggattttctc ctcgacgagg ccagcgtgac ggcgacggaa aacctgctca tggccgccgt
    19381 gctggccgag ggcgagacca cgctcttcaa cgccgcctgc gaaccgcacg tgacggacct
    19441 cggcgcgctg ctggtccgca tgggcgcccg gatcgagggc ctgggcacca attgcctgcg
    19501 catccagggc gtgaagacgc ttcacggcgc ggagcacgcc gtcgggccgg accttatcga
    19561 ggtcggcagc tttcttgccg ccgcggcggc cacccggggg gagttgagaa taaccgcgcc
    19621 gggcaacctc cgcgacttgg ccgtgattct ccgggtattc gcccggcttg gcgtggagac
    19681 gatcgcggaa gccggcgacc tgcttttgcc ggcccggcag aaacggaggg tgcaacgcga
    19741 cctgggccgc gcgatcccga agatcgagga cgggccctgg ccgcagttcc cctccgacct
    19801 catgagcgtg gccattgtcc tggcgacgca ggcgaagggc acggtcctgt tcttcgagaa
    19861 gatgttcgag agccggatgt acttcgtgga ccgcctgatc gagatggggg cggacatcat
    19921 tccctgcgac ccgcaccgcg tcgtcgtcag cggcccctgc cggctgcacg gcacccgcct
    19981 cgccacgccc gacatccgcg cgggcatgtc cctgctcatc gccgccctct gcgcgaaggg
    20041 ccggggcgtc gtcgaccagg ccgaggtcat cgaccgcggc tacgagcgca tcgacgagcg
    20101 ccttcgcgcc ctcggcgcgg atatcgtccg ggaaggatga actcgcgatg agcaaggccc
    20161 tggttcccat cgaacacatc gagaggaaga tagttctgct acgcggacag aaggtgatgt
    20221 tgagcaatca ctaggctgaa ctatatggag tttcgcgcaa ggcactggtg caagccgtca
    20281 agagaaatgc ggaaaggttt ccggatgact ttatgtttca gcttgcacag caagaaggtg
    20341 caatcttgaa gtcacaattt gtgacttcaa gttggggtgg ggttcggcgg gcgaggccct
    20401 atgccttcac ggagcagggc gtggccatgc tctcctccgt gcttcacagt tcccgcgcca
    20461 tccaggtgaa catcgcgatc atgcgcgcct ttgtccggct gcggcagatg ctctccgcgc
    20521 acgcggacct ggcccggaag ctggcggaac tggaagggaa gtacgacaag ccgttccgcg
    20581 tcgtcttcga cgctctgcgc gagttgatga atccggcgcc tccgccgcac aagccgatcg
    20641 gttttcaggt ccgggccccg cgcgcccggt accgggcctg tccggcccgt tcgctgtcgt
    20701 aacaaaatgc ttcacgggtg tgaagaataa tgttgcactt gaccgaagag gaatagagag
    20761 gagcaaccgt gatcgatcaa gtcatcgtcg tggcgtacct ggtggtcgta ctggtcctgg
    20821 ggctgtgggc ggggcggggc atccggacgt tgcaggcctt cgccgtcgtg aaccgcagtt
    20881 actcctcctg ggtcatcttc gccaccctgg cggcgacgtt catagggggc gggttcacca
    20941 gcgggaacgc ggccaaggtc ttcctcttcg gcatcgccaa catcgtcgcc ctgtggggct
    21001 tcagcttcaa ggaactgctc atcgcggcct tcgtcgcgcc gcgcagcggg gcgttccgga
    21061 actgcatctc ggtgggcggc atcatgcggc gccagtacgg gaggacaggg caggtcatca
    21121 ccgggctgct cgggatcatg gtctgcgccg ggatcacagg ggcgcaggtg ggcgccatgg
    21181 gtacggtctt ccataccttc gtcggcacgt cgcgcgagtg gggcatcgcc atcggctgcg
    21241 gcatcgtgat cgcctattcc acgttcgggg gcatgaattc cgtcgtggtc acggatatcc
    21301 tgcagttcat actcctcgcc atcagcatgc cgctggcgct gtggctcggc atccggcatg
    21361 tgggcggaac ggaagcgctg gtccaggcgg tgcccgccgg gcacctgtcg ctcttctcgc
    21421 acatgacgcc ggtcgcgctc gcgtcgctgt tcctgtcgtt cgtgctcggc gaaaccctgg
    21481 tgccgcccta tgtccaacgc ctgctgatcg ggaaaaatcc gcgggcggtg gcggaaggct
    21541 ccctgttcgc gggcttgttc tcgatcccgt tcttcgcggt gaccgggatg ctcggcctcg
    21601 tggccgtggc catccagccg gacctggact ccaaccaggc catgccgttc gtcgtcaaca
    21661 cgctgctccc ggtcggcctc aaagggctcg tgatcgccgg cctcatttcc atcgtaatgt
    21721 cctcggcgga ctccttcctc aacgcggcgg cggtggcgtt gatcggcgat gtcatcaacc
    21781 cgcttcggcg acaggagctt tcggaccgcc agggcctgtg ggtggccaag atcgcgacgc
    21841 tgctcgtggg attgggcgcc gtgctcttcg cgatgaagat cgagaatgtg ctcgatatcc
    21901 tgctcttcac gtataacttc tgggcgccgg tcgtcctcgt gccgctcgtg gcgacgatcc
    21961 tgggcgtgaa ggccaaccgg aagaccttcc tggtcggcgc cttctgcggg gtcgtcgcgc
    22021 tttccatctg gcactggctg ctcaaggatc cgcaggggat cgacggccta gtgatcggcg
    22081 ttgcggccaa cctggtcgcg ttcagcgcga tgcaccggat cgacgtgctg acggggccgg
    22141 aagactgacg gcctcgtcag gcgtttccgg cgatgggcag gtggatgcgg aaaaggctgc
    22201 cccggccggg cgcgctgtcg acggacaccc ggcccccgtg ccgttctaca atcttcctca
    22261 cgaggctcaa gcccagcccc gtgccctcgg tgtcgcccgc cgacttcagg cgggtgaagg
    22321 gctggaacaa ccgcttctgg tcctcggggt cgatgcccgg cccgttgtcc cggacctgga
    22381 aaaacgccga gccgtcctcc atgccggtgg ctacggcgat gcggccgccg tgcggcgtgt
    22441 acttgatggc gttggcgacc aggttgctca tggcctgccg gagcagaacc agatcgcccg
    22501 ccacgggcgg gacgacggtg acgggggaga actccagccg ctgggatcga gacgcggcgg
    22561 tttcgcggag cggccggagc gcttcgttgg ccacggcgtt caggtccacg ggctgccgga
    22621 ccagttcgcc ctcctcggtc acgcgctcgt agtccaggat gccgctcacg agatcgcgca
    22681 tgcgcatcaa cgccttgcgg acctcctcga gcagggcgaa gtgctcccgc ggcagggcgg
    22741 gggagccggt gagggcgagg tcgaggtacc ctatggccgg gctcaacggt tgccgcaggt
    22801 catgggcggc catgcggcgg agggcgtcct tgaattcgtt cagctcccgc agcgaggcca
    22861 tcaggcgcga gttctggatg gcaatggccg ccatgccggc gagttccttc atccactcca
    22921 cttcctcgtc cgaaaagccg cgcgtccggc gcgagagggc gtacagcacg ccgaggacct
    22981 cgtcgccctg gcggaccggc acgccggcgt aagccccgat cccgttttcc gggatgatgg
    23041 ggttggccgt gaagggatcc ttccgggtgt cgggcacgag gcagggctgc ccgtggtcca
    23101 cgatccagcg cgtggccccg ccgctctgcc ggacgcgggt cgccacggtg ccgccgatgc
    23161 gcgtggtgga ggcgccggtc tcgaactgcc tgcgccgggc gtcccacagg accaggctgg
    23221 cgtcctcgca gtccaggagc aggcgggtgt gccggatcac gccctcgaga acgtccgtca
    23281 actccagccg cgcgttgatg tccaggctga cgcgcagcag cgcttcgtag atgttctccg
    23341 gcctgtccgg tcccgtcata tcccgctccc gcggaccggc gggctttcgc cgccgggttc
    23401 aggccttgct cttggcgaag aacgcgtact tctgggggtc gcccatgaag cacaggccca
    23461 tcgtctcgcc cttgacgtcg tcgtagcgga tgccccgcag ctcgagcgcg accttctccg
    23521 ctgtcagcgg cagcgacttg atccgcagcc ccgtggcgtc ctcgatggcg ttggcgacga
    23581 tcggcgcgga cgggatcatc gtatgctcgc ccacgccgcg tgccccgaac gggccgtccg
    23641 gctgcggggt ctcgatgatg atcggcacga tggcgtccgg gaggtccagc gcggtcggga
    23701 tcttgtagtc ggtgaagttc gggttcagca gcttgcccgc gggcgagtag cggatatcct
    23761 cgtagagcgc cgtcgccagc ccctgcagca tcccgccgac cacctgtccc ttgaccaggt
    23821 ccgggttgat cgcctgcccg acgtccacgg ccagcacgac cttccggacg cgcatcttgc
    23881 ccgtttcctt gtccacctcc agcacgatgc ccgccgcgcc gacggtatag tggacgttcg
    23941 ggtggccgcc ctggctggtc tccgggtcgc ccgtggcgga cgtgaattcc ggcatgaaca
    24001 tcccgctggc caggatcggc ccgccgagga aattgccgcg cggcgtctgg atgccgttga
    24061 tgacgaaatc cttgaaggcc agcgcgaaat ccggcttcgc gcggcacttc acgcgctcgt
    24121 cctcgaggta caagtcctcc ttcgcgaacc cgtgcacgcg gtggacgacc tcgatcatct
    24181 tcgcgcgggc ctcctccgcg gccgccttga cggcgttgcc gcagccccag gtcacgtgcg
    24241 agccgacggt ctgccactcg taggcgttgc ggtcggtgtc cggcgtctcg acccggatct
    24301 tctccggcgg cacggtgagc acctcggcgg cgatctgcgc catgaccgtc aggtagccct
    24361 ggcctatctc catgcccgag acgctgatgt tgaccgagcc gtcctcgctg aacttcagga
    24421 acgccgccga cgcggcgttg ggcggcatcg cgggcgcctt ccagaaacac gcgaacccct
    24481 tgccgatgac cttgttgggc gccgcggcct cctccttctt gccccactcg atctccgcgg
    24541 cggttttgtc aatcgcgtcg accagcgcgc acgggttcat gtgcgcgccg tagggcaggg
    24601 tgtcgccctc gcggatcgcg ttgcgccgcc ggaactcgac ggggtccagc ccgatcatct
    24661 tcgcgatccg gttgatgtgc gactcgagcc cgaacatgaa ctccgagtag ccgaacccgc
    24721 ggtacggccc gcccgggggc aggttggtgt acacgcacac ggaatcgatc ttcacgttcg
    24781 gcacgcggta cgggccggtg gcggagaggc cgaccgcgtt cacgacgttc gcgccgtact
    24841 cgacgtacgc gcccgcgtcc cagtgcaggg tgtgctcgat cgccgtgatc gagccgtcct
    24901 tcttcacgcc catcttgatg cgcgcgatga cgccctggcg ctggtaggtg ttgtagaact
    24961 cctgctcgcg cgtccagcgg accttgaccg gcgcgccctt gaccgccagg gccagggccg
    25021 cggcgatgat ctccatggag acgcccgcct tgccgccgaa cccgccgccg acgtacggcg
    25081 tgatgacccg gatgtcctgg tgcttcagcc cgagcggcgc cagggccgcg gcgaagacgt
    25141 gccgctgcgt gtgcggcgac tgcgaactcg accacagggt cagccggccg gacaggtcgc
    25201 actggccgac gatgacgtgc ggctcgatcg cgcagtgcgc gtagcgcggc accgtgtacg
    25261 tgtcctcgag gaccgtgtct gcctcgcgga acccggcctc gatgtcgccc ttccgcgtct
    25321 tgcgccagtg cgcgatgttc gtgtccggca ccgggaagaa ccacgcgcag tggccatagt
    25381 tcttcaggtc ggggtggacc agcagcgcgt cttccttcag cgcctccttc aggtccagca
    25441 cgaagggcag gggctcgtag gtcaccttga ccagacgcgc ggcgcgcttc gccgtcacgg
    25501 gatcgcgcgc gatcaccgcg gcgacctgct cgccgacgaa ccggacgcgg tcgcgggcga
    25561 agatgaaccg gtcctgcatg tacaggccga acgtgtacgg gaagtccttg ccggtcacga
    25621 ccttgacgac gccgggcacc ttccgggccc gcgaaacgtc gatcttcttg atgatcgcgt
    25681 gggcgtgcgg gctctcgacg atctcggcgt gcaggaggtt ggggccgaag tccagatcgt
    25741 ccgcgtacgg cgccgcgcct gtgaccttct ccctctcgtc gatgcgcgtg gcggattgtc
    25801 cgacgaattt catggcgttg cctcctcgtc agcgattagc ggatttcctg gccgcgggct
    25861 tgcgggccct cgccgtgcct tgacgtcctg cctccaccgc cgactcgatg atcgcctcgt
    25921 acccggtgca gcggcaaagg tttccggaga tcgcctcctg cacctcgtgc gccgtcggtt
    25981 tcgggttctt ctccagcagc gcggtggcgg ccaggacgat cccgggcgcg cagaacccgc
    26041 actggaacgc attgtgccgg atgaaggatt cctgcagcgg ggtggggccg tcccgcccga
    26101 cgccctccag cgtcgtgatc tcctgcccct cgacctcgac ggcgaggatc aggcagctcc
    26161 ggacgggctt gccgtcgagc aggatcgtgc acgcgccgca atcgccgcgt tcgcagccga
    26221 ccttcgggct cttgacgccg agcttgtccc gcaggacgtc cagcagcacc tcgtgcggcg
    26281 cgacggccac ggtccgggac tcgccgttca gggtgaaggt gatcttcttc atggcgtgtc
    26341 ctcctccatc acgttgatcc cgtaggccgg acccctgccg gcgagccggg cggccgcggt
    26401 cttcagggcc cgccggacca tgacctcgac catcagcgag cgatactcct tcgtcgagcg
    26461 gatgtccgtg atcgggacga tctcctcgac ggcgagtttg cccgcctctt ccagaagccc
    26521 ggccgacagc ttctgtccct tcaacagggc ctccgtccgg ggaccgcgca cggcgatcgg
    26581 cgcgacggcg ccgaacgcca gccggtattc ccggcccgag aaggccagca cggcgacgct
    26641 ggcctgggcc aggtcctcgc cccggtagcg tttcaatttc acgaagcagc cggcggcctt
    26701 cttcgcgggg agggggaccg tcaccgacgt gacgatctcg cccggccgga tcgcggtctt
    26761 gcggggaccc agaaaccact ggccgatggg tacccggcgt ttgccgttcg ggccgcgcac
    26821 gtgaaccacc gcgttgtaga cttgaagcac gggccccgcg tcgcagcagg gaaccgccga
    26881 gcagatgttc ccgaccaccg tcgcgcggtt gcgcacgccg ttcgaggcca ccatcccggc
    26941 catctcgcgc agcagcggaa aatgttgcgc cacgagttcg gactccagca cgtccgagaa
    27001 cgtcgccagc gcgccgagga ccagggcgcg gttcttgaac ccgatgccct tgagctcttc
    27061 gaaccccttg atatccacca gcagatccgg ggcgacgagt gagtcgcgga gccagccgac
    27121 gaggtccgtc ccgcccgcca gcacccgggc gtcgcgcccg cgcttggcca acagcttgat
    27181 cgccttcgac agcgtcttcg gcttccggta atcgaattca cacgcgatag acattacagc
    27241 ctccggatgt taaccgcgaa ttgacgcgaa gtaacgctaa tgatatccgg aaattcgcgt
    27301 taattagcgt tgattcgcgg cttctcgttt tccttttcct ggcggtctat ttcggcggcg
    27361 cgagcgcgat gatccggcac tgcgccgatt ccatctcgat gcccctgatg gggtagcagt
    27421 tgatccacac ccgctggttg ctgaccttgt cgatctcgcc gcccaggttc tccgcgtgaa
    27481 cgagcttctt cgggaacagc ttgaggtgca tgatctggta gtacttcttc ggcgggaaca
    27541 tttcgtccca ggccttgccg taggtgtcct tgagcttctg ctccgcctcg gcgaagcggg
    27601 cgggatgcca ggtgcggatg atggtgttca tcgggtgatc cgcgctgccg cagtccacgc
    27661 cgatccactt gatcttcatc ttcaggcacc actccgcgaa gcccgggccg gggccgggat
    27721 ggcagacgaa gtagcgcagt tcgtcggact gcggctggtc ccaggcatag cggtggtagc
    27781 ccgtgttgat gatcaggatg tcgcccttgc ggatgtccgc gcgcttcatc agcagctcgg
    27841 gcgagtagag gtcgtagtcg ccgacgtcct tcgagatgtc cacgacggcc gcgggcccgc
    27901 accactcctg gatcggcacg gcgccgatgg accgcccgct ggcgtggaag tggatctcgc
    27961 cgtccatgtg ggttccgacg tggttggaca tggtgatgat ctgcccgttg gcgccctggc
    28021 ccatgtgggc gccggcgatg cgcttgaagt actggatgcc cagcggcatg tagctgggcc
    28081 aggggggcgt gtggatgctc aaccgctggg tcaggtcgta gacccgggcg ttattcatca
    28141 gcttgaagaa ggcatcggta ttcatcgggt ggttcctccg tggttgttct gcgggccgat
    28201 catggcgggt attcaacacc tttcaggccg gttcgacaac aaggatgttg cggggagggc
    28261 cggggtggaa aagcgttgac tcctccgcgg agtgcggcca cgctttcttg aggaacagga
    28321 gggcttgccg tgaagataca gctccttatc ctcgcttccg tgattctgct atggtcggcg
    28381 gcccgggcgc gcccggagcc ccccgcggac gttcaactcg ttcagatcgg gcgcggcttt
    28441 gccacgaccg ccgaacggcg ccccggcgat gccgtccgca tccgggtgaa ggaaaaaggc
    28501 gacgggatga aggacgccat cgtgctctcc cgtttcaccc cggcccaggt caagctgacg
    28561 cgcggctcgg aggccggcgt ggagctcaaa cgctggccca aagccgaagt cgaggccggc
    28621 caggtactgg tctatacgtt ggaggaagcc gcgcacatcg gcgtggaact gcagggctcg
    28681 accaacagcc gcatggaggc cgtccgccgc ggcgactctt tcgagctgac gttcacctcc
    28741 atcaccgagc gctgggtgct ggaagtgaaa ttcacctcgc ccgagttttg aagtggccgg
    28801 tgtgcccagt cgcataccta gaccctctag gtatccgctc ccgaacgggt aacgcgcgtt
    28861 ttttcccttg gggccggcgc cgcggccgcc cggccgcgcc gggttttttc cggaccttgg
    28921 aaaacgccgc tacagcgtcc gggcgaagcc ttcaaaggcc ttcttgaagg gctccagcgg
    28981 ggcgttgagc acgcccgcgc cgacctggcc gacccccgct tccttgtggg ccacgcccgt
    29041 gtcgatgaac ggcaggatgt tcttttcgac caccttgacc acgtcgatgc ccgtcggcgt
    29101 gccgcggaag ttgagcgagg ggatctggta gacgttgttc tccgccgccg tgatctcgta
    29161 catctgccgc gtgtagttca gcgcgtcgtt cgccgtgccg ccgacgaact gcacgatcgc
    29221 cggcgccgcc gcgatggcga agccgcccag cccgttggtc tccgtgatcg cgctgtcgcc
    29281 gatgtcgggg ttggcgtccg ccttggtgta gccggggaag tagagcgcgt ccggcacgtc
    29341 cgccggtccc gtgaaccacg cgtcgcccgt gccggcgagc tgcacgccga agtccgtgcc
    29401 gttgcgcgcc atgacgacgg cgacgctgct gccggggacg ttccgcccgg cgtccaggcc
    29461 cgccttgctc gcgggcatgg acaggttcag gaagaagtgg tcgttcttgt tgatgaactc
    29521 cagcacgtcg gcggtggtct ggggatcccg gcccgtcgtc acgatcgccg gggccagcgc
    29581 ccggtagagc agcgaggtcg ccgcgcggtt gcggttgtgc acctcgtcgc ccatgtgcag
    29641 ggcctgcgcg atcaggttct tcaggtcgat ccgcccgagc ttggcaacgg ccttcttgag
    29701 cgccgggtac aggacctctt ccatccactt cagccgcttg atcacgtcct cgccgtaggc
    29761 cccgtagcgc aggaccttgc cgaggccctc gttcagggtg cagtaggccg tattgccgtg
    29821 ctcctcgttc ttcaccagga acacgggcat ggaggcggac acgatgccgg ccatcgggcc
    29881 gacggcgtgg tgctcgtggc agggcgcgta ttcgatcgcg cccgaggcgg cgagttgagc
    29941 cgcctcggcc ggggacttcg cgcggccctc gtacatcagc gcgcacatga tcccgccccg
    30001 catcgggccg cacatccggt cccacgtgat cggcggcccg gagtgcagga tcaggttggg
    30061 cttcatcccg gggatgacat ccagcgcctt ctcgagcccg acgaggacgg gctgggcggc
    30121 caggatcttg gccgtgacct tcttgttggc ctcctcgatg cgggcgcggt gggcgcgcac
    30181 ggcccggagc agcgccggct cgacgtccac cggcggcttc cagtccacct gcaccgccgg
    30241 tacgccgacg gaatccaggg cttccttgaa cgaggccagg ccgaggttga cgactttcag
    30301 atcgctgcgg aacaggtcgt tgattttcat ggggatctcc ggagggaact acgaaacacg
    30361 cgaaacgcgc gaaaggggtt ggtggacgaa acgctcgtgt tcgattttgg ggaagtggcc
    30421 gaagttgacg agcagggcca attgtttgcc tgtggatttt aggtaattga tggcctgcgc
    30481 ccggtgctca tccgtcaact gcttcacggc tttaatctca acaataatgt catcgaaaca
    30541 ggcgaaatcc ggttcataga cctgtttcag tttccggccc ttgtactcca gtccaagacg
    30601 cggctgttcg acaaaaggga tttcctgatc cgcgagttcc aaggccaggc attcctggaa
    30661 aacagcttct agaaatccgt tccctttctc tttgtaaacc tcgaaacacg ccccaataat
    30721 gcgatagctt tcatctttga acagtatcgt tgtcttcatt ttcgcgcctt tcgcgtgttt
    30781 cgtagttccc tcttctccgg gctcacaggg tggctacgat gaagcctgcc atgaaacacg
    30841 cggcggcgtt gctcggcagg acgatggccc cggcctgctc cagcgcctcg atgacatgcg
    30901 gccggttctg cggatcgcgg tccgtcccgg tcaccgaaca gacgaccgcg actttcttcg
    30961 ccgcctcgcg gaccaccggc gccagctccg aggcggggtc catgtgcgcg ccgaagccca
    31021 gcacgacatc cagcaggatg accgccgtct ccgggtcggc ggcctcggcc tcgatgcgct
    31081 tgttgcgcag cgagaagtcg atcatggggt gggggcggcc gacggtgaac tcgtcctcgc
    31141 cgtagtccac catcgcgtgg ccctcgctct tcagcgagtc cttcagcttc gccgcgccgg
    31201 cggtcggggc gttggagaac acgccggata gaccgagctt gcggaaaatg acctgggctt
    31261 cggcgcagaa cgtgccgccg ctgaacagcc cgcgcacgcg cttccgccct ttcagccggg
    31321 cggccagttt tttcgcggcg ttcttcattt cgaggtcgcg ggactcgacc tggcgccggg
    31381 cctcctcgta ggactcgccc ttggcgcgcg cgaggaccgt tagggcggcc tcctcgagcg
    31441 tgtgcgggcc ccccgaggtc tcgtcgccca ggaacatgga aacgacaggc ttcttgatgc
    31501 ccttcacggc cgtttcgatg gccttgagca cgtcggggtg cggcggcttg gaaacgagaa
    31561 gaatcgcctt ggtcttctcg tcggccgcca gggccttgag gccctcgatg aacatgatgc
    31621 cgccgacgtc cttcttcacg tcgcgcccgc cggtgccgag ggcctgagag atcccgccgc
    31681 cctcgttcgc gatcaggcag gtcacctcct gcaggccggt gccggccgcg gccacgacgc
    31741 cgatcgggcc gcgcggcacg atgttggcga aggccagcgg cgcgccgttg atgatcgccg
    31801 tcccgcagtc gggccccata accagcaggc catggtcccg cccgaatttc ttcagcgcta
    31861 cttcggtctc cagcgagacg ttgtcggaga acagcatcac gtgcagcccg cgctcgaggg
    31921 cctcctgcgc cacgtcgccg gcgtagcggc cggcgacgga gatgatcgcc aggttggccc
    31981 cgggcagcgc ctccacggcg ccctccaggc tcgaggggcg cacggcctcg ccgcccgccg
    32041 actttttctt cgtcgcctcc ttcagctcct tctcgaccgc ggccatggcc ttttccacgg
    32101 cggcctcggc ctccgccttg acggcgatca gcaggtcggt gtcgcccgaa gcgtcgaagt
    32161 cggaaaggta cagtcccgac gcgccgagga tccccttgtt ggtctgcgtg cccatgacca
    32221 cggcggcgtc cgtgacgccg tcgagggcgg ccacggcctt gccgacgcgc atcagggtga
    32281 tggagtcgta gtagcttccc ttcttgatct tacctcgaac catcatgggc ctctcctgtt
    32341 gggcgttggt tgttgaaagt tggaaccttt gtgcaccgcc agcaccagcc ctgtcaagat
    32401 atccgcgccc gaggtttcgc cgtgggccag gacgcggttg gcggcggcgc gcacggtttc
    32461 tgcctcggcg cctcccagcg ccgtgagcag gtttttcatg ggctcattca cccgcccctc
    32521 ggcggcgagg gcgaggaagg actcggacag aacgtttcct cccgggaggg acggcttcca
    32581 cgccgtccaa ggccgcggtg gaacccggcc ctcccggagg cgccacccga tcagagcccc
    32641 cgcgagcagg tcgtccccgg ccggcgtaag gccgaacccg caaccgcgga gcagccgcac
    32701 ccccggcgtc ggatcttcac cgaagagctt cgacgcgccc gcctgaattt gccggatcat
    32761 gttttgctca aagccgtctc caaaatttcg gatgcgggac gcgtccagca ggaaggcgag
    32821 gcttttcggg tgggccgcgg cgcgcacacg attgaccagg acttcgacca tcgccggctc
    32881 cggcgggatt cccggaaggg cggagtcata gcgcgggccg agggaataac gccggccgcc
    32941 gaggagcagg gcgtcgggtt caacggtgag cgaatcgtcc tttgggagct cgccgtcgac
    33001 cacgatgctg atcgggccgg acccgacgga tcggtcggat aagacgacca ggcgcgttgg
    33061 gctggtgaaa ttcgccgcgc gccggaaccg ggagtggagg cggtacgcgc ccgcctccac
    33121 ccggtccccg gcgcttacaa ccttcatgcg gcggtgaaga ccatgccgag gttcttgccg
    33181 aactcgctcg agcggacggt ctcgatattt atccggacgg ccatgccggt cttgggcttg
    33241 gggattctca cctggccggt gacccggatg ccgttctcga gctccacgat ccccagcgtc
    33301 agttgcgcga cgttgaagtc cgcgggcagg gtggagaggt cggtataggt cagcagcttg
    33361 cccttcttcg gcaggggcac gggatcgaac ccgtgcggct cgttggcctt gcacttcttg
    33421 cagaccatgc ggaaggggta gtgaagctgg ccgcacttcc ggcacttata accgtagacc
    33481 tgaagttcca tggatcagtc ctccctcttg aagacgaggc tgacgacgtt gttgccgaag
    33541 ccgccgaagt tggtcgtgag accgatcttc gcgttcttca cctgccgctt cttcgcctcg
    33601 ccgcgcagct gcgtgacgat ttcgtgggcc tggccgatgc cggtcgcccc gacggggtgg
    33661 cctttgccct tgagcccgcc ggacgggttg atcgggatct tcccgccgag cgcggtctcg
    33721 ccgcgctcga gggcgaggtg gccctcgccc ttcttgaaga aaccgacctc ctcgctttcg
    33781 gcgatctcga ggatcgtgaa ggcgtcgtgc aactccgcca cgctcacgtc ggagggcttg
    33841 atcccggcca tcttcatggc ctgcgcggcg gcgccgcgca cggctagcag gtcggtcggg
    33901 ttctcgcgct cgtggacgca gtgcgtgtcc gtcgcctggc cgacgccggc gagccggaca
    33961 agcggccgct tgaccttgcg cgcgacctcc tcgcaggcga ggatgacgca cgcgccgccg
    34021 tcggagaccg ggcacatgtc gaagaaccgc agcgggtcgg cgacgatcgg gttgttggtc
    34081 agcgcctccg gcgagtcgag gatgccctcg atcgtgatcg gctggtggag gtgggcgaac
    34141 ggattgagca tggcgttggc gtggttcttg accgcgacca tcgccaggtg gcggctggtg
    34201 acgccgtact tctccatgta gaggcgcgtg aacatcccgg cgagcgaagg cagggtgacg
    34261 ccgtagatgt attccgccag cgggtgggtg agcgtggcga cgaagtccgt ggcctcgagg
    34321 ttattcacct cgcgcatgcg ctccgccccg gcgaccatga ccgtgtcgca caggcccgac
    34381 gcgacggcca tgaagccggt cttgatggcg gacgagccgg aggccggccc gttctcgatg
    34441 gtctccgcgc cgatcggcgt caggctcaac gtgtccacga cggcgctggc cagcgccgtc
    34501 tggccgttaa cccggccgcc gcccatgttg gagacgtaga ggtgatcaat cttcgggttc
    34561 cccgcgtcct ggagagccat catggccggg taggccgcga tctcggagat gggaaactcc
    34621 agccgggaga agctggtgat gccgacgccg acgacgtaga cgttgcgcat ggtcacacct
    34681 tcccttccgg ttcgcccagc ttggccagcc tctggcggct caacagcagc gccgatgccg
    34741 tgggatcgtt catggatcgt gtcgcgccca gaagatccgc gggcggcttg ggcgggttgg
    34801 acggcggcag gatggctgcc tgggccttct tcagcagcgc ctccggcacg gggcggcgtc
    34861 cgacgacgag cgtgtcgcgc gcggcgtcga acagcggctt ggtcagcgcc cacgacggca
    34921 cgccgccgag ctcgtgcgcc acgttgaacc gcttctcgag gtggtactcg atcatccagc
    34981 ggcggaatcc gatgggcgac tcggcgacga tcagcacctc cttgtggccg aggatgtcca
    35041 tgtggtattc catcttcgcc gcgaacgcgt gcgcgccgac gcgcagcccg cgccgcaggg
    35101 cgagggtgtg gtaggacacg ccgacgtcct tgttgagaga gcggccgttg acgatggccg
    35161 ccaactcgcc gttgatctgg gcgatcagga cgtactcgtc ctgcacgcgg tgccggtagt
    35221 agcccagcag ctccgcgtag acgcggacgg cgacgatgtc gtagtagtcg cgctcgacgt
    35281 ggatcagcgg ctcgacatgc ggcaggagat cggggatctc ctcgcggctg acctggcgaa
    35341 cgaccatgtc ctccccgctg gccaccttca tgaacttggg cgggaagggc gggagcggag
    35401 catccacggg acgcaggatt ttttccagtt ctaccgccat atttcctccg gggtgtgcgg
    35461 gcgattcaac tcgcccttgt caaggcacct taacgccgcc gcctcccggg gcgcaagcag
    35521 gaaggcgata aatgtgtatt gcacttcggg cgagcgagga gtacggcttt taccttttca
    35581 cggaggagtg gaaatgagcg gtcgggagtg gatgttcatg gtgttccagg tgatcggggg
    35641 gatgagcctc ttcatcttcg ggatgcacgt gatgacccgg gggctgcggg cggcggccgg
    35701 ggaggccctg cgggtgatcc tggcgcggac cggccgcagc cgtgtccagg gcctcgtgct
    35761 gggcaccctg gtcggcttcc tggcccacag cggcgcggcc acgacgatgg tcgccggctt
    35821 cgtcaacgcc gggctgatgg gcctggcgga gtccgtgccg cccatgctcg gggccaacgt
    35881 cggcacggcg ctctccatgc agctgatctc ctttcacatc ggcgcctact gctggatggc
    35941 catcggcatc gggttcatct tgagcatggt cgcgccgggc gagcgcgccc gggagtcggg
    36001 caacgccctg ctgggcttcg ggctgctgtt cctgggcatg acgacgatca gcgaggccat
    36061 cgcgccgcac aaggacatgc tggcgccctg gctggcgcac atccacggcg agacgtggac
    36121 gggtcggttg atcggcgtgg cgatctcggc ggcgctgacg gcggcggtca ccagcagcgg
    36181 ggccatcatc gggctctgct tcgcgctgat cggcgccggc gtgttcacgc agttcgacca
    36241 ggtctttccc atcgtgctgg gctcgcacat cggcacctgc atcgtcgcgc tcaccgcgag
    36301 cgcggccatg aacgtcgagg cgcgccgctc ggccctcggg cacctcgtgt tcaacctgtt
    36361 caacgtcgcg ctggcgctgc tggcctatcc cctcgtgcgg gcggcggtcc tgtggagcgc
    36421 cgacggcctg acgcgccagg ccgcgaacct gcacctgttc gtgatgaccg cggtttcgtt
    36481 gatcgtgctg ccggcgagca agccggtcgc cggcctgctc cgccggctgt ggccgtcgag
    36541 cgaaccgccg ccggagccca gccacctgga tgtcgaactg ctggagaaac ccgagcaggc
    36601 gctgtgcgct tcgatccggg aactccgccg catggccgcc ctgtgcgtgc gctgcatgga
    36661 tacgaacggg gtcgtcatgc tgaagggctg ccgcaccagc ctgcgccgcc tctacgccga
    36721 cgaggaggtc atcaacgagg tcaagaaggc catgtaccgc tacctcggcc acctgaccga
    36781 ccgctatctc tcccgccgcc agaccctttt cctgcagcac gtcgggcggt gcatgaagga
    36841 cctcgagcgc atcggcgacc acctgaccgc catcgccgac atttccatcg agcggtggaa
    36901 gcacccggag gccatggtgc ccgagccgat gttccgcgtg tggttcgacc tgttctgcgc
    36961 ggcgaaggaa gtgctggtga tgaccgagca gtcgctcaac ccggacctcg acgatttcca
    37021 gggccgcgcg ctctccatcc tggaggcccg cgaccgctac gtcatcatga gcatggatgc
    37081 caaggcggag ttcgccgggg ccgccgaggc caagaccatc acgccgctcg gcgggtacta
    37141 cttgagccgc tacatggccg acctggaccg gctcgtgcgc catgccaagt ccgtcgcctt
    37201 cgccgagcgg cagcccgact tccgcgtcaa gcggaccaag ctcgaccggg aggcgaaacc
    37261 cgcgggcgcc tatgagccgc ccaccccggt gaaggccgag gattacctcg cccggcttca
    37321 gcggatggac tggctcagcg acagcgagcc ggcgctgccg gaggaaaaga ggcccgcgcc
    37381 ggggggcggc cctggctgaa tcagtcccgc cgccgcggca ggacgcactc gaagagggcc
    37441 ccgggggcgc cgggattgac gagcgccagc tgcccgtcgt gggcggcggc gatgtccgcg
    37501 gcaagcgcca gccccaggcc ggcccgaccc ggccggccgc cggggacgct ggcgaaacgc
    37561 tcaaaaatgc gttcggcgaa ctcggtcggt atgccgggcc cgtcatcccg cacggccagc
    37621 gcggccgagt ccccccgccg ttccgcgctc aaccggaccg tcccgcccgg cgcggcgtgg
    37681 cgtatggcgt tgtccagcag attccccagc agctcgacca gcaactcctc gctgcccgtc
    37741 acgagcaggc cgggtttcgc gcggacctcc agcgacaggc cgcgctcctg gcagaggggg
    37801 aggtagatgc ttttcacctt ctcgatgacc tcggcgaggt ccaccggcgc gaactgcgcg
    37861 cgcaaggcgc cggactccag ccgcgacagc tgcagcagct ggtccacgat ccgggtcagg
    37921 cgctccagtt cggacagcat ggtctccacg gtctcccggt actgggcggt gtcgcgcggc
    37981 tgggtcagcg ccacttcgcc caggctgcgg atcgcggcga tgggcgtgcg cagctggtgc
    38041 gaggcgtcgc cgctgaaccg gcgcaggcgc tgcaccgcgg cctcgtagcg gttgaacgcg
    38101 gcgttgatcg tgtccgccag gtggtatgtt tcgtcgtcgg gcatcagggc ggtttcgatg
    38161 cgttccgaga agcggccggc ccggatgcgg tccgccgtcg cggcgatcgt gcgcaccggg
    38221 gcgatcaccc ggcccgagat ccaccaggcc accaggacca gcggcaggag cagcaggagg
    38281 tccaggatcg tcgcccgcag cacttcctcg aagccctcgg ccagcggttc ggccgggttt
    38341 tgcagccagc cgacgtattc gacgagcgcg tagatgacgg actgggcggc gatgaaaaag
    38401 aagagcaggg caagaagata gaggaacacg cggctgcgca ggcggagtct cacggcgcgt
    38461 ctcccgccag catgaacccc acgccgcgca cggtgcggat cagcttctcc tcgcggcccc
    38521 ggtcgatctt gtcgcgaaga tgggataggt ggacgtcgat gacgttgtcc agcggcgtca
    38581 ggcgccggtt caccttccag atgtcccgcg cgagcatgtc ccgggacacg ggatgcccgg
    38641 cccactgggc caggtaggcc agcacgtcga actcccgcgc ggtcagctcc accaggcgcc
    38701 cggcccgccg cacctcgcgg tgccggcggt ccacttccag gtccgacacc cgcgcgacat
    38761 cggcagccga ttcccgcgcc gcggtgcgca gccgggcccg gacccgggcc agcagctccg
    38821 aaaacgaaaa gggcttggcc aggtaatcgt ccgcgccctc ctccaggccg atcacgcggt
    38881 cggcgatcgc gtcgcgcgcg gtcaggatga tgaccggctg ccggtgtccg ttccggcgaa
    38941 gctggcggag ccactccacg ccgtcgccgt ccggcagcga gagatcgaga atcagcaggg
    39001 cggggggcag gcccgccatg gcggcggtgg cctcggcgag aagcctgacg gttcgcacct
    39061 cgaacccggc ctcgcccagc ccgcgcgcga ggctttcgga cacccggggg tcgtcctcga
    39121 tcacgaggag cccggaaggc gcgggatcgg cgctggattt atcacgctcc atcttaagca
    39181 atcttaagac aaatccggtg gaatgccagc gacatccgga ggaaggtgct gtccaaccga
    39241 gcatgagcac cttctttcac acctgggaaa aactgttcga aaccggccgc cggctgcgcg
    39301 acgcgctgcg cgagcagcag gagtgcatcc gcgaggtcta cgcgcgcctg gccccgtacc
    39361 tggccacgcc ggcggatacg cccgccggct acgccgtgcg gcccggcgcc atccccggct
    39421 cgttctacgg ctggcgaaag aacatcttct cgacgctctt ccactccgtc taccacctcc
    39481 tgggcgtccc ggcgccgcgg cgcatgctgt acgggaagat gatccacttg ttccgcgtct
    39541 gggtgacgtc cgccgacaac ctgctggacg acgaggacaa ggtggttgtc ccgctcgaga
    39601 tgccgggctc gtcgcgggtc atgcggcagg tggtggccct gatggccgcc gaccgggtcc
    39661 tcgccgagct gctggccgag gcggccgcgt cggggctgat cgaggcggag cagggcgcgc
    39721 tcctggcgcg ggaatcgctg cgccgcctgc tgcccagcgc ggcgcaggag gctacggagg
    39781 agggcggcat cggcgagcgg ccgccgcccg agcacgtgct cggggtgatc caccggctca
    39841 agacgggcct gctgttcaac gtcgcgttcc tcgggccgga cctcctggag ccggacctcg
    39901 ccgccgggcg gcggcaggac ctgaaggagg ccctgatgca gttcgggctg ggctgccagc
    39961 tgctcgacga cgtgcgcgac ctcgcgcgcg acctgatcga gggccggcac aattacgtcc
    40021 tgtccgtgct cgcgcacacc gagcccgctg cgctcgcgga cctgcgcgcc gcggcgcgcg
    40081 acgtgtccga ccggctgtac ctgcgcgtcc cgcacgccgc gctgccggcg gtccggcgcg
    40141 ggctgggcct gatgcgcggc gggctgcgga cgctgggcga ggccggcctc ggcttcggcg
    40201 gcgccgaggc ggactcgatg gcccgctcga tgctggccgt cctggacctg gaggggctgc
    40261 cgcatgcgta agaagaaaca gcatccgcgg tccgcgtgcg cccggcgcac cgccggcgtc
    40321 acgctggacc acgccgcgcc ctggtacgac tggctggccc cgctgatgac gctgggcctg
    40381 gaggctcggc tccagcgccg cgtggtcgcg ggcctgcgga cggaccggcc gatggccgcg
    40441 ctcgacgtcg gctgcgggac cggcacgctg acgcgccagc tccacgcggc gataccggcc
    40501 ggcccgggcc ggcgcgtcgt cgggctcgac gccgccgagg cgatgatcgc cgtcgcgcgg
    40561 cgcaaggcgg ccggccgcga gggcctggaa ttccaggccg cgctggccga ggaactgccc
    40621 cacgccgacg ggacgttcga ccgcgcgatc tcgacgtttt tcttccatca cctgaactac
    40681 gacctgaagc ggcgcgccct ggcggagctg tggcggatcc tccggccggg cgggcaggcc
    40741 gccatcctgg acgtggacat cccgtattcc tggttcggcc gcctctgcgc gtattccggc
    40801 tactggctgt tccgccagcc ggagatcaag gagaacatca ccggtcgcct gcgcgaggcg
    40861 ctggacgctg gcccgttccg cgggcactgg cgcattgcgt cccggcactc cggctatttg
    40921 agcctgttcg aactgaagaa acccgaaacc aagaaggaga ccacatgaag agagtcctga
    40981 tgatcctggc ggccgccatc ctggcggggg gcatggcggg cgcctcgacg cccgacgacc
    41041 cgatcggcgc cgcggtggag aagtacttcg ccgggacctt cgccggcctc aaggccgtgg
    41101 cggaccagaa acccacggag gacaccctgc gccgcgtcat gaaaccgctg gcggagaaga
    41161 cggacggctt cttcggcggc acgctgatcg acaccgactt tgtcatccgc gaggtgtact
    41221 tcaagcgcga tttcctggcg cgcggcttcg atctcaagaa ggtcgagcag ttggactact
    41281 tctgggagct catgcgcaag gcaccggctc cgcagttgag cgagccgggc cacggcaaca
    41341 tcatgcagcc gcgcctgatc gcgatgcgct atcccgtgat caccgacggc cggctggaat
    41401 cggtggtcag catcatggtc cgtaccgggg cgttcctgga ggcgaccggg
//
LOCUS       DAOOTK010000019        39552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_750_length_39552_cov_6.509203, whole genome shotgun sequence.
ACCESSION   DAOOTK010000019 DAOOTK010000000
VERSION     DAOOTK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 39552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39552
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_39552_cov_6.509203"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2381
                     /locus_tag="PK634_03955"
     CDS             <1..2381
                     /locus_tag="PK634_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOW96920.1"
                     /translation="DDTTLLRAGEVRVEVRRREPKLEDVRIISRDGTLLFDGAASVPA
                     KAFLPEPSASFASWALADQPRLVPPAWGALPPPNDADPASGWNLGNPARDVYVFLPGA
                     GGYRQLVRDLLRLTGPVPLPPLCAFGLIDSRYYPYRQDEALEIIDEYRRRGIPLDIFV
                     LDTDWRVGASHGYEVNRELFPDLPGFIRAAHQRHVRIMLNDHPEPADPRALSPAEIGY
                     RARGLTSLLEQGADFWWFDRNWHTVLGSPAEGLGKETWGMRLYHDIVAAYRPDRRPMI
                     MSNADGIDNGHLNNPPHPAAHRFPIWWTGDTRASWADLERAVRNAVRSGTLSLLPFLS
                     DDLGGHHDTPDPELYVRFVQYGTLSPVCRLHCSYKLHRFPWMFGEPAGTIASEYIRLR
                     YRLLPTLYAAARQASAEGTPMLRRGDLEWPGHAEAASDTQYLLGPDLLVAPILRAATP
                     LAPIPTEFLRGADGTPGLQAEYFGNDRLAGEPAARLVEPLLNHGWFGRPPAPGMEGRG
                     FSARWTGTLGPVPEDGKYRIGVRTDDGVRLWLDDRLLLDEFEQSGEVYKWRDVDLKAG
                     QRCALRVEYRNLGTWHAMFELLWGRPNGPDSPRPVWVPPGMWMDPWTGARIEGPAEQA
                     IPAALHQVPMLIRGGGILFSTPLRLHTGTALWPEIRADLAWYPGDFDTRRELYEDDGF
                     SIAYRQGAGRVTPLRFRRARGTVEIEVEPAAGGLADAGGRRWLFRVHGLTTRPADVHV
                     HDATLLAVAWGAGAGLLPFEASASWARAARPVLEIDVEQSRAGTPVRVEFRA"
     gene            2465..4189
                     /locus_tag="PK634_03960"
     CDS             2465..4189
                     /locus_tag="PK634_03960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW96921.1"
                     /translation="MAAKPFESRIQDLIYNVDVGIGLALMKVGLYLLFVLITMLLYTA
                     TQFRGLKEPAAMDAAQLGRNLATHHRFITQNIRPASLWYLSRKNPAGGLKMDRHPDIV
                     NAPLWPAVLGAAFKVAGTAFATEKPSAVFPPEQWVIVPLGHFFTLMTGLVLFFFGRRL
                     FDRRVAVLGVTLYFLSDTVWRNSISGLGLSLLTFLVVTAFYLAYLAAGQQTEDGRPRG
                     QALLLLAGSAALCILAFYTRYAAAVFAPALMVYIGLSFRKKGWTWAAVFLLVFLLGIA
                     PWLARNALVCGNPLGLAGHLALNGTDSFTANGFDRTLKPSLEFEKVFRDLQTKWMGGL
                     SRFYRQDLWRTGDGLLFGLFVAALFFRFVNTAAHRLRWCLALGIGLLWPVAALFGEET
                     SRLLTVCWPVVLLFGLAFFFVLLDRLQLQARLFELALIVALVVTSALPLAFALLPPRA
                     GPPYPPYFPPYIMHVTRMLTPDEMICSDMSWATAWYGDRTSLLLPSELDSFYEINDYH
                     RRIYGLYFTTITRDKPFVSGLVTGPERTWYPILEGRIPTDFPLNQGFPINNMDQLFLT
                     DRERWSRR"
     gene            complement(4198..5847)
                     /locus_tag="PK634_03965"
     CDS             complement(4198..5847)
                     /locus_tag="PK634_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW96922.1"
                     /translation="MHGSIRLVVGRAVISVLTLLALPSPAEEDPNSPFLQDLTEAYVT
                     LGRANRAREEESWEQALSLYEDAHAQYRRLHNLYPDRQPDIVQYRLADCANQIEALRR
                     RLGPNKRTGPRPASEAAPSRDPASADFADENRSLRERLAAAETNAARTAAMSGQDIAR
                     LTAELQAAEAQRVADRASLEAEKKQLEEARGEIARLKQDLDGAARNLKKIETERDSLR
                     AARETLEETAKSLARELETARARLEAEQAAAAPLVEARQAEQEQRKLLEQAEAQLAEA
                     RQATEALRASLDQSETQRAQLTERAAERQEEAKKDRQARKEQAAELEAVRSQLAEEQA
                     ASASLRERIEAAEARIQELQNSASSPAAGISPSPPDRAAEKIRRAMELERTGKLEAAL
                     TIYRSSPGRPDMVKGAGRCLLKLNRAGEAVDVLTAEAGPAQDAESRLLLGIGLCTLRR
                     YAEAAATLEAALPSDPQNPALHNALGVARIGQGKLAEARQELEQAVALNPDLGDAHLN
                     LALVLAADPSGDRAQVRDHYKRALELGVAPEPHLEKTLGKP"
     gene            6130..6885
                     /locus_tag="PK634_03970"
     CDS             6130..6885
                     /locus_tag="PK634_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOW96923.1"
                     /translation="MSLAPGQQQRYARHLALPQIGEAGQRKLLAGRVLVIGLGGLGSP
                     AALYLAAAGVGCLGLADPDVVEWSNLQRQILHFTPDLGRPKTESAADKIRALNPDVRL
                     RLHAGRFDRAHAAAMLREYDFVVDATDHFATKYFIAEACHAAGRAYAHAGIAAFAGEV
                     MTVLPGHTACYRCLFDGPPPPDPASPLGLLGAVPGVIGCIQAAEAVKYLLGAGELLVN
                     RLLEFDALAMTFREIPVSRNPACPLCGRPDSRD"
     gene            complement(6895..7860)
                     /locus_tag="PK634_03975"
     CDS             complement(6895..7860)
                     /locus_tag="PK634_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOW96924.1"
                     /translation="MSGEWILLFLCLAAAWFFSGIETGLVAINRLRLHHLVRRKVPGA
                     EILQYFLQNPDILLGTTLVGTNIAVTMASTLAVNLGADAFGSSGAWLASAAVTTVLVV
                     FCEYLPKAWFQAFPSHRSLPFAPVLKAARWVLMPASAPLMGLVRLLTPGRPRDDEKSR
                     PLVTREEIVHLAGEGKSTGILTPAEHRMIHEVIELKTKACREIMTPRDRMVYIRQDAP
                     APALLDLARAREINRFPVQDPEKKTFVGIVHIFDVLADANSADKRVRDYMRPCQFVAD
                     HAPVDHILPRMRVTRQHMILVTDDRFEVIGLVTLEDVLDEVLGAL"
     gene            complement(7857..9080)
                     /locus_tag="PK634_03980"
     CDS             complement(7857..9080)
                     /locus_tag="PK634_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOW96925.1"
                     /translation="MTVILIIAMVGLMICSGFFSSSETVFFSLNPLALRRVSQRNPTI
                     GAWIHTLLSRPTQILSTVLIGNTIVNVGIAAIGFLLADRAFPGRGEAISIPVVTVALV
                     IFGEAGPKRIGLLHAERLAVWFAPIMLLLIRALAPLRFLLERLMKSFEPMFRPSGRTL
                     SEEEFETVLEISQEQGILNADELAMLKAIVDLEDLKPGDVMTPRVDLVGVDLGGAPED
                     WIAVARKARRNFLLLYRGQVDNVEGFLDVRKFLLDPEHRIESARQPPFFVPANTPLNQ
                     VLMQFQRDRLRIAVVVDEYGGVAGVITRGDILEEITGEIYNELSKPRPLFQQAGPHRW
                     LVDANISLEEINRKLRLNLRARGADRLAGWIAARAGHIPEQDDVVEADGARVTVMQTI
                     RRRVTLAQIEKVEGP"
     gene            9220..9816
                     /gene="msrA"
                     /locus_tag="PK634_03985"
     CDS             9220..9816
                     /gene="msrA"
                     /locus_tag="PK634_03985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOW96926.1"
                     /translation="MKLWIVPALLLAAVAKGEPVMHENPQENTAIATFGGGCYWCMEA
                     VFQQAPGVLSVVSGFMGGTVRNPTYEQVCAGGTGHAEVIQVRYDPARVSYEQLLEWFW
                     KAHDPTQRNRQGNDVGTQYRSVIFYHDEAQRQAAEASRAALDQSKVFARPVVTEIAAA
                     AEFYPAGKYHQDYYRRNPSQPYCQFVIRPKLNKLGLKE"
     gene            complement(9832..10848)
                     /gene="ruvB"
                     /locus_tag="PK634_03990"
     CDS             complement(9832..10848)
                     /gene="ruvB"
                     /locus_tag="PK634_03990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOW96927.1"
                     /translation="MTDRFVTDTLNKADTEFDISLRPSRFADFVGQPKVKERLELFVE
                     AARGRGDVLGHVLLSGPPGLGKTTLAYILANAMGVNIKVTSGPVIDKPGDLAGLLTNL
                     EKGDFLFIDEIHRLQKSVEEYLYAAMEDYVLDIVIDQGPHSRSVRLNLPRFTLVGATT
                     RSGLLTAPLRSRFGLAARLDYYDTSALQEIVTRSARILNVDVEPAGAREIAGRARGTP
                     RIANNLLAWVRDYAQVKAGNRITREVADRALAMLDIDEHGLDEMDKRILETLLQKFSG
                     GPVGVHSLSVSVGEEPDTIEEVYEPFLIQQGYLKRTPQGRVATERAYEKFGLKPRGQA
                     PELF"
     gene            11224..11505
                     /locus_tag="PK634_03995"
     CDS             11224..11505
                     /locus_tag="PK634_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOW96928.1"
                     /translation="MVIGLLQATLSIPESHSLKDKRMVLRSLKDRALNKMNVSVAEVD
                     QQDLWQAATLAFVTVAAEKDVVEKRLAEVSEHLRSDPRAILLDYETHLL"
     gene            complement(11441..12112)
                     /locus_tag="PK634_04000"
     CDS             complement(11441..12112)
                     /locus_tag="PK634_04000"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOW96929.1"
                     /translation="MNRKEQLVLASASPRRRAFLKSLGFRFRIVPPELNETPNRGERP
                     AHYARRLSEDKAAEVAARIRGPAVIIAADTIVVQGRRILQKPRGRADAGRMLEALSGR
                     WHAVISGLCVARAAGGRVVRRRSRTVRTAVEFKRLTREEIAGYIATGEPMDKAGAYAI
                     QGVGSFMIRAIRGSYTNVVGLPVAELVDILEQDFGLVLHPRGLKASASRSRAGSRGGR
                     TAGAR"
     gene            complement(12141..12371)
                     /locus_tag="PK634_04005"
     CDS             complement(12141..12371)
                     /locus_tag="PK634_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96930.1"
                     /translation="MARTAGRLAFFKLCFEDFASGRENCWIFDFYGILADAGGNLKRS
                     YATGSEDSHPNGSAYRALAFRFRGLFEEAGWP"
     gene            complement(12398..12616)
                     /locus_tag="PK634_04010"
     CDS             complement(12398..12616)
                     /locus_tag="PK634_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96931.1"
                     /translation="MPRTVVGKCCVCGSVRREGRWEAPDRLEAEDAFYTHGYCPACLN
                     RALTELALIDLHLASWPAPRPREAVSAR"
     gene            complement(12806..13636)
                     /locus_tag="PK634_04015"
     CDS             complement(12806..13636)
                     /locus_tag="PK634_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96932.1"
                     /translation="MKRSITWGVVVVLAWLGSSLSAMAQSPLRLVFAAPIEGAAGGVS
                     WSYSDIYVHVDSMDGLGGSWGAVVHHDGGAGGSWIDTPMSLVGHYGTHMLFYARVPRA
                     RMRFAIRATWSYAVSGMTTTRDYWDNNGGADYSVIVQGGAVDGIVGGNVGLVVASNYC
                     RTVSGLPAIPRLCNQYVAGTLVVQSTAPSVVAGIRVTTDNWRHFTDIPVSSAASYGFG
                     YGSAQVRVCRFEQRIGTRMTKPNRTAKFAVYFRDALGREFWDNNFEQDYTVRAGGTIR
                     "
     gene            complement(13743..14975)
                     /locus_tag="PK634_04020"
     CDS             complement(13743..14975)
                     /locus_tag="PK634_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOW96933.1"
                     /translation="MKRTWCALFLLAGLGGPGLRAQELCHWNTDHRFFRLNGDEGSEF
                     VNLFIHDGGFVWTNYAVGGTVTVQSALWLDDNSWVSMEHLKVPISEPFQFWQYSDPTC
                     VARTGQTNSYLEGDDGDLEPGHTWPDRFTVQADTNLVVDNLTGLMWARNADSGHNNWD
                     FMVSAVYWSVAPGDYEDWRLPTVRELESLLDLKRVLPALPAGHPFNNVQLNYWTSTSD
                     AADTNRAWVVSLGDGTIARWNRTNTSPHAWAVRAHTTGKMPVPRTGQTNSIVLGDDGH
                     LRPGVPWPIPRFTVMTDTNLVFDNLFGRMWVRSLGMTPVADWHSAVSYCSGLAFGGYA
                     DWRLPSRREAWSLIDFGNASFLPEGHPFNGLHETFFWTSSTYRADTTRAWGIEGGHLG
                     AHIKIDIGGGIWPVRGDW"
     gene            15423..17660
                     /locus_tag="PK634_04025"
     CDS             15423..17660
                     /locus_tag="PK634_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96934.1"
                     /translation="MMTGNAFRIPGPRGRSPVLLAWSGAVFLAAAGLALAGLPANGLV
                     LHYSFDADNGTTVPDESGGGNLGLITTNLEWTAFGISEGAYRFHGSNEYITAGPMFNW
                     TNFSQGFTLSFWTRQIEAEFRGVYYAGTTGGSGAQTNRFDLGCNIISMGDITFRLFDD
                     EGDAIIRQYPATGVDDGEWHHVALLVSSNANDIHLMLDGADLAVGAGSGTVFQLNGGL
                     KFPFVIGTSSDGGGGPSHTNSPNGYLDDFRVYSRRLASNEAVTLFRMMEDEPNDTVPQ
                     ARPIAPGQTKHHTFKALDDEDWEFFYTFTNYTNYVFSTKNTGTNVDTQLTIYRNLDDG
                     TLGLVTSVNNTAMGSGLGESWTLNNFSNDLYYVKISAPTGLYGGGGGMGPARRAARSD
                     GDAGFGEGSEYQSELVATATPGVRIVYVAAPDNGRQAGVWAKVDSTTLYFPARNEGLA
                     FPGVSEALHLVEVGFKPGVRPWEHPTRPGQLDNPNSSFGNPHWVDLRGSSMFTICVFR
                     CESLNYQQATVRDGYTGAHLSDAAVTCISDSAPFTGTETRRGHAYETSYQQLWRTLAD
                     GSFPTNLMLYPTTWDIRLNRDGYVTRTITDAVAAVGWGYTNNLGDLRLYPVDANFNGI
                     ADAWELAHFPIEAFPIDPAGDLDEDGLSNWAEYMADTDPTNDADRLDLSLTAENDTLR
                     LRWQGRPWRRYRIGMQAVVAEEGWDSATDTVTHSGTSGWEQVWSDVNWLDNSQRVYRL
                     EVLPP"
     gene            17657..19411
                     /locus_tag="PK634_04030"
     CDS             17657..19411
                     /locus_tag="PK634_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96935.1"
                     /translation="MKKRLGKTARWAMSLAMALPAAGKEFTIATPLGEIQVDVPHLTP
                     QGAVVTPEAASVSVFRPPTIFSAPLPSGSGARALGLAGAFTAVADDATAASWNPGGLV
                     QLERPEASFVYRASGEDDTHRVDSETMSVGSDSFHSSGLNYFSGVLPFEAGHRNFVFS
                     INYQEAYDFEQRFTADYTGRSSRRNSETAQASASSTFSETYSLGNGEVTLTSEITTET
                     IRTLDQALASELLTSLDFDQQGLIDAVTPAFAVELTPKLSAGGAVNLYGDDLLGRNPI
                     RSTSTARFAGATDSRVDITERQTTSGAYYYDAVAHVPPVGNFPGFDVPFPRVEGVYEP
                     FTDTTASRDGDLLYYEGTITEENRTDDLTGQNATLGALWTVSRLLGLGFTVDLPWTAE
                     ARQQRTVRETITTYDASRTRVVDVSETRESETRDVEYRFPLYWAAGAVLRLADEFYVT
                     MDVSQTLWSDFTFQAEGEEKLNPLDGTPHGQNPLDDCWSVRAAMEYMLFFSRVEIPLR
                     AGVGWEERPAIGEPDEFWNFSLGTGISLGSGPRRVILDLAYLCSVGENALGSLLPGTG
                     MTSDVEEHQVYVSGIVHF"
     gene            19532..20077
                     /locus_tag="PK634_04035"
     CDS             19532..20077
                     /locus_tag="PK634_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOW96936.1"
                     /translation="MQPEQDSDGRIRDALSRDDPVALDWIWEAYAARLLAFASAILCS
                     HHDAEETLQNVFLKIARNRDRLADAVSLKAYLFTMTRNEACTLIRKRGRREVSVDPRD
                     LWLVPAGNPEPGLPEDAEAAAQRLATLPVRQRDVIVLKVFREMTFDDIAGALDISPNT
                     AASRYRYGMDKLKRRMNRGRP"
     gene            20074..20526
                     /locus_tag="PK634_04040"
     CDS             20074..20526
                     /locus_tag="PK634_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96937.1"
                     /translation="MKTMPDESILKSFRVPGPAPELRARILASARLEWKKRVLPSEWS
                     RLRNPLRALAASIAVAVLGQWANGRLAASPNASVARVREPLPRELTELADRPAVFLIP
                     AVPAADLSSRQAQLRDLLEGPAPLPDMPAPQGQTYDNRQTKGSPASCC"
     gene            20542..21891
                     /locus_tag="PK634_04045"
     CDS             20542..21891
                     /locus_tag="PK634_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96938.1"
                     /translation="MKIAAVRFIKALVALLVALLLLHTSLLILSGLSLRNAYKELRQA
                     GRPIGAADIIPPPVPAGENAAPLYTSAFALLDAKTIGEEPLFSRLAQAARDFSGEPES
                     EEKQAALEQLLGLEDVARALEMIEQASARPKCNFNLQYDQGAALLFPHVNGMLKISRI
                     LAARTKLEARRREGDRAWSAVETNLRMADALRDEPALISALVRIAIFQAALTSAREAA
                     DLAAPDDQTAARLMARLATADDPAPYVLAMDGERILMGEWAFNHVRTQKFFDCLGMEG
                     EACNWKMQLYNLLIGYRPARQADQATYLRAMGEVAQRAARPYWESPVEPDREWDRAMP
                     WYGAISRLILPALGQSRIRAATLQAEVRITRAGLAMIRYKTAQGRYPATLDELVPAFL
                     DEIPRDPFTGEPLVYRPEGDGFVLYSLGQNQQDDGGTAESPDNRDTKTFDIVWRRAR"
     gene            complement(21896..22666)
                     /locus_tag="PK634_04050"
     CDS             complement(21896..22666)
                     /locus_tag="PK634_04050"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOW96939.1"
                     /translation="MPGKVLRAAAALLVAALCSCAPERREPAAGVALVIGINAYEGNW
                     PALSSARPDAEAVARVLEERYGFEVRRLFDAAATRAAILGALDQLCLLDDRATVILFY
                     AGHGVLDEDGEGYWIPFGAVRKEAGIAHADLADRLLVAAPRRVLVISDACFSGSLLGR
                     RLWDGTGNAPDDGRPAKYVIASGDLAPVPDAQGAHSVFAQALLEALTNPPGGALSVAD
                     LVHALQRRLQRLTGQVVRAGPLAPETDGAFVLRGKPGR"
     gene            complement(22656..24629)
                     /locus_tag="PK634_04055"
     CDS             complement(22656..24629)
                     /locus_tag="PK634_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96940.1"
                     /translation="MTIHRVRFLLLACGLLLGGCYSPPPDVGTRADELRFHNWWNYYR
                     RGTERLRAGQTEQAREDFERCLGLRPGAKFGYTRDKWRARTYGVHVLEGYYPHRELGV
                     CLFLLGDVAGAEKHLDLSLRQQPSGRAKHYMNRARRARLQGAKLAPPRVMLTVEDAVV
                     WTAARSFTVTGRAEGEGNVRELRVNGEPEFIELAEPALDFSRTVALEAGANDIRVEAR
                     DLLGREAVTTLRIMADWRGPELLIRSLAREGGGWRARGVCRDDDRLESATLLTNSHPA
                     RLLKPSASRPGVEVDVTIAPGRPAWLEATDRAGNTLRVDLARLVRALQENEPVQSASL
                     QPVLRASNEPEERDLVRPELLINGPLVATVYQDAYYLDGQAGDRGGLAAVLLNGRELL
                     SSDERGALSYTFSQRLPLAPGENRFELAARDRSGNRSVKKLVLTRETPPALDRQYRLS
                     LGLPPALCEPGLKETVCRRLREAFDEEPVRFNVMDGALDWPAGPAETEAGVDLAPYLA
                     LVRKAGTELLAVCSVAREGAGRSIYARVVDPADGQILFVEDVYSEAMEEELPAQVAGL
                     VSKIERRFPLFRAAVIQASRGRIVMDKGAEDGVRAKARAIVAVPGAGEVRMAGGVPVE
                     LQVTRAERSRGIAEPVASAARDVVQEGDVVFAR"
     gene            complement(24721..26091)
                     /locus_tag="PK634_04060"
     CDS             complement(24721..26091)
                     /locus_tag="PK634_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW96941.1"
                     /translation="MDSSWEMEDEFLARLRTGAQEAWTQFFFRFDPFIRSVVCWSKWG
                     FDSTAREDVMQNIRTEIPRAIANYRGEQPFRIFLRGICIHRCADEVRRQIKQRERYLP
                     MDVPTSDGETRHLDYAAGEDFDPVYAIAEAERAALLRQLLEDMGPPCNQALKNFYLED
                     LSYKDMAARSGEPISTIGNRLARCLERLKEKVQKIPFYRIYVPRKATVPVDETDMNTN
                     ESGRPELWKHVHGQADTAEAEALRASVRTDATLRRELEARAQLDARFRRLLKVSEQSQ
                     AALEERIGELWERSRTLAAKPESRILTFWKPALVALAATLALVIGAVTMSPEGLVWMA
                     PGLDAGAVRGPDGVEQAFYSEAELQDFAQSLQQSVASTYADRDGPSGWIARWTGRQRS
                     VRSTIRAFPDGRILATLEAFRSPGDSATGTWSESFANAEDFSLNAGDWAGRVAEEIVA
                     DSEARN"
     gene            26157..33278
                     /locus_tag="PK634_04065"
     CDS             26157..33278
                     /locus_tag="PK634_04065"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW96942.1"
                     /translation="MKMRALFALLAAWGLCGHATVTYATTYYVRAGAAGGGTSWADAR
                     GNLQGAIEACAASGGGEVWVAAGTYYPTLTPNGGSGTREVHFSLRNGVTVYGGFPATG
                     SPTMALRDPENYPTICSGAIGDPDDFDNVYHLFYHPTLAALDETAVLDGFRIEDGRAD
                     GVSDDEGAGMYNEWCSPTIRNCVYTRCYASNCGGAMHNAYSSPRIQGCAFDDNRSGAA
                     AGAIRFIRGSPVIQDCVFTNNVAESYGGALQNMNTTLLVSNCVFANNRATGEMPISHG
                     GAMCSQDSFITVVSSLFKRNAADLGGALAELAGSTHHYVRCTFIYNHSSSDGGAFYGL
                     RAPDVRLTECVLKDNLADYGNGGAIYANDSSVTAISCALDGNEASEGGGLFCDEGSEA
                     LIANSIVSGNVADWAGGSGIHIHWYATCTFVNGVVYGNRTYEHDGGAGVYICSANQSY
                     FYNSIIRGNRDNDDEYVNFSICDELAWPGVAFCNATYDGGVFPGSGNSSADVQFMDVG
                     DPDGADDLWLTADDGYRLREDSPCREAGTVEALPADLTDVDGDGDTAEDIPLDAIRHL
                     RVQGARVDMGAYEFGSAVQPTYSVDFVLGAHGLRTGGGALHQDVVEDHATVAPEVIGI
                     DGWMFTGWDADFSRVTADLTVNAQYSLTAHTVRFDLGAHGARVGGGELVQNVPHGAAA
                     AAPLIRVGVGWYFRDWDTAFDHVTGDLVVHALYQEISIPGDIVYVKAGATSNGSSWAQ
                     AMGDLQAAIDFLGDRGAGQVWVAQGTYVPNSWSWGGWTERTVHFALRENVEVYGGFPA
                     TGTPGMGDRDWMAHPTICSGDLGALYDYSDNAYHVFQVRGVENAVLDGFVIEWGNAND
                     LESPHSGGGGMRIEYGAPTIVRCAIGDNRARYGGGVYCKYGRSPTFTDCRFSDNAAIL
                     KGGGMRNAAGASPDLDACVFHSNEAEWGGGMANDLSYPDLKRCRFEQNTATDWGGALH
                     NAGGYVRMINGLLVQNEAGDGGAVFNSAGLFWAFNTVWHGNRASFSGGALYNEMDADS
                     YLYSSTVTSNTAGSGGGSFSWDADQHVYNSILWGNTGGSFVNMEAAPAVRYSLIQGGY
                     AGGIGVLNQDPVFANPLDPNGADNEWLTADDGLQLQADSIAINFGAASYVTADTMDLD
                     GDADLEEAIPLDAAEGARTVGFRPDLGAYEYPALVTHTVTFNLGVHGHRTGGGALVQE
                     IERHGAADAPVFTTDIGWAFTGWDRAFTDVTAPLAVNATYEFAFETGDICYVRQGATG
                     NGTSWGEATGDLQGAIDAMSSAGGGQVWVAAGAYKPNSFPNGGSGARQQHFSLRNGVA
                     VLGGFPATGDPGFGDRDPDGQLTICTGDIGLSDETSDNTYHVFYHPASAALAAAAVLD
                     GFVIHGGRADGSAPHDSGGGLYNSSCSPTLRHCVFLFNYAQNGGGLYNLSSSPKLTRC
                     RFTMNTAANQGGGMRNYTASSPSLANCVFNGNAATDGGAMYSHSAAPMLVNSVFSGNT
                     ASDEGGGMYNSAAAAMVYCSTFEGNTAAVRGGGIYNTSSSPTVENAILWGNIAPAGSN
                     LYDGASSSSMVGNSTVGGGWAAGYNISSADPLFQNASDPDGADNRWATSDDGLRLAIW
                     SPALNAGGNGSIPTDGHDLDDDGNVAETLPIDLAGKARVTDDTVDPGAYEFEVPVHTV
                     TFDLGAHGMRTGGGELVQRVEHGASASAPALDTEEGWAFTGWDRVFDAVTADLLVTAQ
                     YEFRTFDVTFDLGARGARVGGGELAQVVGYGSAATAPRLTVADHWYFTGWSAAFDEVT
                     SDLAVAAVYSQILPSGGICYVKQGGTENGSSWDKAMGDLQAAINEMRLAGEGQVWVAA
                     GTYRPNSWPNGGTGDVRAMHFALANDVEVYGGFPAAGNPGMVDRAPAANITLCSGDIG
                     AAGVSTDNVYHVFHHGLEAALEPSAVLDGFHLRHGQATGVFPHNDGGGMLNRASSPTV
                     RNCAFSDNVADWGGGMQNINSSSPLVEDCSFINNQGFWGGAMGNQGGSHPSIYDSVFQ
                     GNRATRGAAVYSFEASFQAIADCVFEQNQATENGGALYLDGACGIVSRCEIRGNHAGN
                     SGGGVVLAGNSRLFCSLVAGNSSTMSGAGVYLVTTGRIEYCTIAANVAGKHGGGLHMD
                     GRGVVTASIIYSNSAAAEGPNGHDASALGTYDHCCTTPEVAGAGLVTDPPAFVAPAAG
                     NFRLSAGSPCVDAGPAGAEVVTDLDGRPRPLDGDADGASVLDIGAYELLNADADSDAD
                     EMRDGWELEHALNLLDPADAAENPDKDPFTNRDEAIADTDPFDPGSYLRITQLLFMPE
                     PTLFYWPASHRRAYTVCASANLRTAAWSDLPGATRVVPPASGLQSFHLFGRETEPRGE
                     CYRLRVSYP"
     gene            33359..35686
                     /locus_tag="PK634_04070"
     CDS             33359..35686
                     /locus_tag="PK634_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOW96943.1"
                     /translation="MKERGRLWLCLVLAAAPMSSGAERGGRLYPIMQPDWETRQEWFA
                     SHRAAPRAVIDPQVQSRLLGSVSLLSHVQYTPAERDQSSCGNCWAWAGTGVMEVDLDV
                     QQGVKDRLSVQFLSTCCGKTKCGCQGGWLADVATFYQGKGYTIPWSNPNGQFQNQGGD
                     CGCTCASIGISPNYPLASISYAAIETQGIASNTAIANIKNVLNQNKAVWFGFFFPRSS
                     DWTHFQTFWNNQAESVVYVPPSGVCGATWDSGGGGHAVLVVGYNDDDPNPANHYWLIL
                     NSWGATAGRPNGLMRWTMYLNYNCQSYDRSTWDYNYYWQTLNISWAASSRPVVATSNA
                     TDVTSSSATLRGAVNPRGSATTRWFEYGLTAAYGSSTAGRSAGSGSANSNVSEAVSGL
                     APANTYHFRLAASNAAGVSYGSDLTLTTLAGGATAPDATTDAASSIGPTSAVLHATVN
                     PRGSSTAVCFQYGPTEAYGFTTPTQQVGAGSAPTSAARTISGLSTAALYHARVKAWNA
                     GGTTYGADRTFSTLGAAVTLFSENFEFGGVSPPGWTRQYVRANDHGASIDWYLAYGDG
                     ALWRDMPAAPHGGSWNVFFYDEDYNAWTAVISPPISFGTGYAQSTLTFWHYMAEDYWW
                     GDQDELLVYMGPTTNGPWTRLATYSNNITAWTQQTINLPNPTGTVYLSFDAISHWGYG
                     VAVDDVEVRVKASADPGPVIGRGAAGDGRLVLRWDAAAGEQYRVEYTTNLAAGAWQED
                     AAAGTLAPSDNEVWYTNTPAAGATNRFFRVRRIAP"
     gene            35726..36526
                     /locus_tag="PK634_04075"
     CDS             35726..36526
                     /locus_tag="PK634_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOW96944.1"
                     /translation="MTAPRCILAVLGLALCSGCGTLPDGRRWGHEATLRPGWPRVGQS
                     AWRAAIDPQTWGPLAAAALLQIDNADHEISDWAIEHAPLFGSPEEAEDAVGWTGQALD
                     TAWYASMLLAPGGETAGEWTLNKLRGAAVEFGAVGVAGFTVDTLKEEVGRTRPNQENK
                     RSFPSNHAQRSAVDVTLAARNLDATPMPPAVRWTAKTGLYGVGAVSAWSRVEAAGHYP
                     SDVLAGWAIGHFWGAFLHDAFLWRGEPPVLIELAPAPGGASLILRARF"
     gene            36593..37456
                     /locus_tag="PK634_04080"
     CDS             36593..37456
                     /locus_tag="PK634_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96945.1"
                     /translation="MATTEVEVLRFDNLEQYLFVKNGNYLYKVPYHGGFAVLKVYWCS
                     RGWFERISKTFDNIVIAGQTSFMPRARLRTEQQAMAIWREAGFRVFQIYENVRVEGIE
                     EDGYALYEYVPGRHFHKLLPDESVPREERIRLFRQFLVEWHRRHRLAIDRREPRLIHE
                     NGDLKHVMDYNGELVWFDFEMCYRSRSHVEDLVAREILAYAKSIGDFMKGELFELFLN
                     EMVEHYPDKDLLGHAYTVTFRNRNPVIRVGRNLEFLLRERAKRWNSKYNMALMIRDRL
                     ARLHGQGAASA"
     gene            37431..38276
                     /locus_tag="PK634_04085"
     CDS             37431..38276
                     /locus_tag="PK634_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96946.1"
                     /translation="MDKVQRLLEQLRPWQAERLDGSARGSGLNAVWKIRLPAGPAILK
                     TFSRRRGRAQTLLTEINHRLGGLTSFTARGRRRTEAAILALWRDADLDAPRLLDDPPG
                     LALALPHLCMEFVDGELLSHRLADESRPLAERDALLVRFLRAWSDRHALADERRYPRL
                     IQEHGSFEHVMVAGDRLVTFDLEVSFQRSRDVRACIVAEIVGYVRSLFRLLPAPLAGH
                     YLGVVVREYPRREYLEEVPRRLLRHPNPVLRAIHFLDRRLLRKPGKWHKYRVAELLMS
                     ELKMR"
     gene            38288..39433
                     /locus_tag="PK634_04090"
     CDS             38288..39433
                     /locus_tag="PK634_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW96947.1"
                     /translation="MRIVHLISYFRWTERVEPAADLAIAQQQLGHDVRYLCGHNQGAA
                     PEDCIQGRAARKGLAFDDRFTLDKHFRLLSALRDIAGLRKYMAEFKPDVVHCHQDNAH
                     LLAAFALRGLAPRPLLVRTFYEPGRPSSPGRYWLFCHRRTDGLIVLNEATRAQARGRF
                     GRQPDRVAVILPGIDVDGFANRRDLGRLEHVNVPPGAFVAGMITHIGKVQRLDLALEA
                     VAALTPRHPELRLLIVGRGKPDRWVHEPARRLGIADRVILGGYCRYEELVKAYHTMDA
                     LVYPRHGTDESCRAVREALTAGVPVVAPGIAIMPDLVKEGETGFLADFDSASLANALE
                     KMLRLDPAARARMSAAAVADARARFSRVKQAGKVLEFYERLRGLPRM"
BASE COUNT         6325 a        13620 c        13272 g         6335 t
ORIGIN      
        1 gggatgatac gacccttctg cgcgcgggag aggtgcgcgt cgaggtccgc cgccgggagc
       61 cgaagctgga agacgtgcgc atcatatcga gggacggcac gctgctgttc gacggcgccg
      121 catccgtgcc cgcgaaggcc ttcctgcccg agccgtccgc ttccttcgcg agctgggcgc
      181 tggcggacca gccgcggctc gtgccgccgg cgtggggcgc gctgccgccg ccgaacgacg
      241 cggatcccgc cagcggctgg aacctcggca acccggcgcg ggacgtctac gtgttcctgc
      301 ccggcgcggg cggctaccgg caacttgtcc gcgacctgct ccggctgacc ggcccggtgc
      361 cccttccgcc gctgtgcgcg ttcgggctga tcgacagccg ctattacccg tatcgccagg
      421 acgaggctct ggagatcatc gacgagtacc ggcggcgcgg catcccgctg gatatcttcg
      481 tgctggacac agactggcgc gtcggggcct cgcacggcta cgaggtgaac cgggagctgt
      541 ttccggacct gccgggcttc atccgcgcgg cgcaccagcg ccacgtgcgc atcatgctca
      601 acgaccatcc cgagccggcg gatcctcgcg ccctgtcgcc cgcggagatc ggctaccgcg
      661 cgcgggggct gacgagcctg ctggagcagg gcgcggattt ctggtggttc gaccgcaact
      721 ggcacacggt gctcggttca ccggcggaag gcctcggcaa ggaaacctgg ggcatgcggc
      781 tctaccacga catcgtggcg gcctaccggc cggaccggcg gccgatgatc atgtccaacg
      841 ccgacggcat cgacaacggg cacttgaaca acccgccgca cccggccgcg caccggttcc
      901 cgatctggtg gaccggcgac acgcgggcgt cctgggccga cctggagcgg gcggtccgca
      961 acgcggtgcg cagcgggacg ctctcgctgc tgccgttttt gagcgacgac ctgggcgggc
     1021 accacgacac gccggacccg gaactgtacg ttcgtttcgt tcagtacggg accctgtcgc
     1081 cggtgtgccg cctgcattgc tcctacaaac tgcaccggtt cccctggatg ttcggggagc
     1141 ccgcggggac gatcgcgtcg gagtacatcc ggcttcgata ccggctgttg ccgacgctct
     1201 acgcggcggc ccggcaggcg tctgcggagg ggactccgat gctgcgccgc ggcgacctgg
     1261 agtggcccgg ccacgcggag gcggcgtccg acacgcagta cctgctgggc ccggacctgc
     1321 tggtcgcgcc gatccttcgc gccgcgacgc cgcttgcgcc gattccgacg gagtttttgc
     1381 gcggggcaga cggaacgccc ggcctgcagg ccgagtattt cggcaacgac cgcctcgcgg
     1441 gcgagcccgc ggcccggctc gtggagcccc tgctgaatca cgggtggttc ggccggccac
     1501 cggcgcccgg catggaaggg cgggggtttt ccgcgcgctg gacgggaacc ctcgggcccg
     1561 tgccggagga cgggaagtac cggatcggcg tcagaacgga cgacggcgtg aggttgtggc
     1621 tggacgaccg cctgctgctc gacgagttcg agcagtccgg cgaggtgtac aagtggcgcg
     1681 acgtggacct gaaggccggc cagcgctgcg cgctgcgggt ggagtatcgc aatctcggga
     1741 cctggcacgc gatgttcgag ctgctctggg gccggccgaa cggcccggat tcgccccgtc
     1801 cggtctgggt gccgccgggg atgtggatgg atccttggac gggcgcccgc atcgagggtc
     1861 cggccgagca ggctattccg gcggccctgc accaggtgcc catgttgatc aggggcggcg
     1921 gcatcctgtt ttccactccg ctccggctcc acacgggcac ggcgctttgg cctgaaatcc
     1981 gggcggacct ggcctggtat cccggcgatt ttgacacccg gcgggaattg tacgaagacg
     2041 acggtttctc gatcgcgtac cggcagggtg caggccgcgt aacgcccctg cgctttcgcc
     2101 gggcgcgcgg aacggtggag atcgaggtcg aacccgccgc gggcggattg gccgacgccg
     2161 gtgggcggcg gtggctcttc cgcgtgcacg gattgacgac ccggccggcc gacgtgcatg
     2221 tgcacgacgc gacgctgctg gccgtcgcct ggggggcggg agcggggctg ctgcccttcg
     2281 aggcttccgc gtcgtgggcg cgggcggctc ggccggtatt ggaaatcgac gtggagcagt
     2341 cgcgcgccgg cacgccggtc cgcgtggaat tccgcgcata gggggcggcc atattcggaa
     2401 ttgcccggcc gccggagacc ccgtagcata agcggcggat aggcagggag cgacaggagc
     2461 agccatggca gcgaaaccgt ttgagtcgcg gattcaggat ctgatctaca acgtcgacgt
     2521 cggcatcggc ctggcgctga tgaaggtcgg cttgtacctg ctctttgtcc tgatcaccat
     2581 gctcctgtac acggcgacgc aattccgcgg cctcaaggag ccggccgcca tggacgccgc
     2641 ccagctcggc cggaacctgg caacccacca ccgcttcatc acgcagaaca tccggccggc
     2701 gagcctgtgg tacctgtcgc ggaagaaccc ggcggggggg ctgaagatgg accgccaccc
     2761 ggacatcgtc aacgcgccgc tctggccggc ggtgctgggc gcggccttca aggtggccgg
     2821 gaccgcgttc gcgacggaga agccttccgc cgtgttcccg ccggagcagt gggtgatcgt
     2881 gccgctgggg cacttcttca ccctgatgac cggcctcgtg ctgttcttct tcggccggcg
     2941 cctgttcgac cgacgggtcg ccgtgctggg cgtgacgctc tacttcctga gcgacacggt
     3001 ctggcgcaac agcatctccg ggctggggct gtccctcctg acgttcctgg tcgtgacggc
     3061 tttctacctg gcctaccttg cggcgggcca gcagaccgag gacgggcggc cgcgcggcca
     3121 ggccctgctg ctcctggccg gcagcgccgc gctgtgcatc cttgcgtttt acacccgcta
     3181 cgccgccgcc gtgttcgccc cggcgctgat ggtctacatc gggctctcct tccggaagaa
     3241 gggatggacc tgggcggcgg tcttcctgct ggttttcctg ctcggcatcg ccccctggct
     3301 ggcgcgaaac gccttggtct gcggcaaccc cctcggcctg gccgggcact tggcgctgaa
     3361 cggaacggat tccttcaccg ccaacggatt cgaccgaacg ctgaagccat cgctggagtt
     3421 cgagaaagtc ttccgcgacc tgcagaccaa gtggatgggc ggcctgtcgc ggttctaccg
     3481 gcaggacctc tggaggaccg gggacgggct gctgttcggc ctgttcgtgg cggccctctt
     3541 cttccggttc gtgaacaccg cggcccatcg gctgcgctgg tgccttgcgc tgggcatcgg
     3601 gctgctctgg ccggtcgccg ccctgttcgg tgaggaaacg tcgcgcctgc tgaccgtctg
     3661 ctggcccgtc gttctgctct tcggactcgc gtttttcttc gtgctgctgg accggctcca
     3721 gctccaggcg cggttgttcg agctggccct catcgtggcc ctcgtcgtga ccagcgcgct
     3781 gccgctcgcc ttcgcgctgc tgccgccccg cgcggggccg ccgtacccgc cctatttccc
     3841 gccgtacatc atgcacgtca cccgcatgct cacgcccgac gagatgatct gcagcgacat
     3901 gtcctgggcg acggcctggt acggggaccg cacctccctg ctgctgccct ccgagctgga
     3961 ctccttctac gagatcaacg actaccaccg ccggatctac gggctgtact tcacgacgat
     4021 cacgcgcgac aagccgttcg tcagcggcct ggtcaccggg ccggagcgca cgtggtaccc
     4081 gatcctggag gggcgcatcc cgactgactt cccgctcaac cagggatttc cgatcaacaa
     4141 catggaccag ctcttcctga ccgaccggga gcgctggagc cgccgctgat ccgccggtca
     4201 gggctttccg agtgtctttt ccaggtgggg ctcgggggcg accccgagtt ccagcgcgcg
     4261 tttatagtga tcccggacct gggctcggtc gccggaagga tcggcggcca ggaccagcgc
     4321 gaggttcaag tgggcatccc ccaggtcggg gttgagggcc acggcctgct ccagttcctg
     4381 gcgcgcctcc gccagcttgc cctgcccgat ccgggccacg cccagcgcgt tatgcagcgc
     4441 cggattctgc ggatcggagg gaagggcggc ctccagcgtg gcggcggcct cggcgtaccg
     4501 gcgcagcgtg caaaggccga tcccgaggag caggcgcgat tcggcatcct gcgcgggtcc
     4561 ggcctccgcc gtcagcacgt ccacggcctc gccggcgcgg ttgagtttca ggaggcaacg
     4621 cccggctccc ttaaccatgt ccggtcggcc gggcgacgat cggtagatcg tcagcgcggc
     4681 ctccaatttc cccgtccttt ccagctccat cgcgcgccgg attttttcgg ctgcgcgatc
     4741 cggcggagac ggggatatcc ccgcggcggg ggacgacgcg ctgttttgca actcctgaat
     4801 acgggcttcc gccgcctcga tccgctcgcg cagggaggcc gaagccgcct gttcctccgc
     4861 caactggctc cgtacggcct ccagttccgc ggcttgctcc ttcctcgcct gcctgtcctt
     4921 cttcgcttct tcctgtcgct ccgccgcccg ctcggtcagc tgggcccgct gtgtttcgct
     4981 ttgatcgagc gatgcgcgga gggcttcggt agcctggcgg gcctccgcca gttgcgcctc
     5041 ggcttgctcc aggagtttcc tctgctcctg ttccgcctgg cgggcttcga caagaggcgc
     5101 ggcggcagcc tgctccgctt ccagtcgcgc gcgggccgtc tctagttccc gtgccagaga
     5161 tttcgcggtc tcctccagcg tctcccgggc ggcccgcagg ctgtctcgtt cggtttcgat
     5221 ttttttcagg ttccgggcgg ctccgtcgag gtcctgcttc agccgggcga tttctccgcg
     5281 cgcctcctcc aactgctttt tctcggcctc gagggaagcg cggtcggcga cccgctgcgc
     5341 ttccgccgcc tggagttccg cggtcaaccg cgcgatatcc tgcccggaca tcgccgccgt
     5401 ccgtgcggca ttcgtttccg cggcggccag gcgttcgcga aggctccggt tctcatcggc
     5461 gaagtccgcc gacgcgggat cccgggaggg cgcggcttcc gatgcggggc gcggacccgt
     5521 ccgcttgttc ggccccagcc gccggcgcaa ggcttcgatc tggttggcgc aatcggcgag
     5581 gcgatactgg acgatatcgg gctggcgatc cgggtagagg ttatgaagcc gccggtattg
     5641 ggcatgggcg tcctcgtaca gcgagagcgc ctgctcccag gactcctcct cgcgcgcgcg
     5701 gttcgcccga ccgagggtga cgtaggcctc ggtcaggtcc tgcaggaagg ggctgttcgg
     5761 atcctcctcg gccggggagg gcagggcgag aagcgtaagg acggagatga ccgcgcgccc
     5821 aacgacgagt ctgatgctgc catgcatggc ttatcctccg gccggttccg cgttgtgaag
     5881 catcctagtc ccggcggcgc gcgcggacaa gcccttcatc gcgccggcgt tgcctcgcgc
     5941 aacggccagg aacccgggcc gcgtcccctt cggaaacccc gcgccacact ttgcaaagtg
     6001 tggcgcgggt cagaacagaa aggcgcgcgg ttattggtta atccgctccg caggctcatt
     6061 ccatactttg caaagtgtgg cgcgctgaaa aaacttgatg cgggccctga cacatggcat
     6121 gagggcgtca tgagcctcgc ccccggacag caacagcgtt atgcccgcca cctggcgctt
     6181 ccccagatcg gcgaggccgg gcagcgaaaa ctgctggccg gccgcgtgct ggtcatcggc
     6241 ctgggaggcc tcggctcgcc cgcggccctc tacctggctg ccgccggcgt cggctgcctc
     6301 ggcctagccg accccgacgt cgtcgagtgg agcaacctgc agcgccagat cctccacttc
     6361 actccggatc tcggccggcc caaaacagag tccgccgccg acaagatccg ggcgctgaat
     6421 ccggatgtcc ggctccggct gcatgccggg cgctttgacc gcgcgcacgc ggccgcgatg
     6481 cttcgggaat acgatttcgt ggtcgacgcc acggaccatt tcgcgaccaa gtatttcatc
     6541 gccgaggcct gccatgccgc cggccgggcc tatgcgcacg cgggcatcgc ggccttcgcg
     6601 ggcgaggtca tgaccgtgct ccccgggcac actgcctgct atcgctgcct cttcgacggt
     6661 ccgccgcccc cggacccggc ctcgcccctc gggctgctgg gcgccgtgcc gggcgtgatc
     6721 ggctgcatcc aggcagcgga ggcggtgaaa tacctgctgg gcgccgggga attgctcgtg
     6781 aaccgcctgc tggagttcga cgccctggcc atgaccttcc gggaaattcc cgtgagccgc
     6841 aacccggcct gcccgctgtg tggccggccg gactcccggg attgaggcac gccgtcagag
     6901 cgcgcccagc acctcgtcca gcacgtcctc gagggtgacc aggccgatca cctcgaagcg
     6961 gtcgtcggtg accaggatca tgtgctggcg cgtcacgcgc atccgcggga ggatatggtc
     7021 cacgggggcg tggtccgcga cgaactggca gggccgcatg taatcgcgga cccgcttgtc
     7081 cgcgctgttc gcgtccgcca ggacatcgaa tatgtgcacg atgccgacga aggtcttctt
     7141 ctccggatcc tgcacgggaa agcggttgat ctcccgggct cgggcgaggt cgagcagcgc
     7201 gggcgcgggc gcatcctgtc ggatgtacac catgcggtcg cgcggcgtca tgatctcgcg
     7261 gcacgccttg gtcttcagct cgatgacctc gtggatcatc cggtgctcgg ccggcgtcag
     7321 gatgccggtg ctcttccctt ccccggcgag gtggacgatc tcctcccggg tgaccagggg
     7381 ccggctcttt tcgtcgtcgc ggggccggcc gggggtcagc aggcgcacga gccccatcag
     7441 gggggcgctc gccggcatca ggacccatcg cgccgccttc agcacgggag caaacggcag
     7501 gctgcggtgc gaggggaacg cctggaacca ggccttgggc agatactcgc aaaacacgac
     7561 cagcacggtc gtcaccgccg cgctggccag ccaggccccc gaagagccga aggcatcggc
     7621 tcccaggttg acggccagcg tcgaggccat ggtcacggcg atattggtgc cgaccagcgt
     7681 cgtgcccagc aggatgtccg gattctgcag gaagtactgc agtatctcgg cgcccgggac
     7741 cttgcggcgg accaggtggt gcaggcgcag ccggttgatc gccaccagcc ccgtctcgat
     7801 gcccgagaag aaccacgccg cggccaggca caggaacagc agtatccact cgccgctcat
     7861 gggccctcca ctttctcgat ctgggccagc gtgacgcggc ggcggatggt ctgcatgacg
     7921 gtgacccggg ccccgtcggc ctccaccacg tcgtcctgtt cggggatatg gccggcccgg
     7981 gccgcgatcc agcccgccag gcgatccgcc ccccgggccc ggagattaag ccggagcttg
     8041 cggttgattt cctcgaggct gatgttggcg tccaccagcc agcgatgcgg ccccgcctgc
     8101 tggaagaggg gtcggggctt gctcaactcg ttgtaaatct cccccgtgat ttcctccagg
     8161 atgtcgccgc gggtgatcac gccggccacg ccgccgtact cgtccaccac gacggcgatg
     8221 cgcagccggt cgcgctggaa ctgcatcagc acctggttga gcggcgtgtt ggccggcacg
     8281 aagaacggcg gctgccgggc ggactcgatc cggtgctccg ggtcgagcag gaacttgcgc
     8341 acgtccagaa agccctcgac gttgtccacc tggccgcggt acagcagcag gaagttccgc
     8401 cgggccttgc gggccaccgc gatccagtcc tccggcgccc ctcccaggtc cacgccgacg
     8461 aggtccacgc ggggcgtcat gacgtcgccg ggcttgagat cctccaggtc cacgatggcc
     8521 ttgagcatgg ccagttcgtc ggcgttgaga atgccctgct cctggctgat ttcgagcacg
     8581 gtctcgaact cctcctcgct cagcgtccgg ccgctcgggc ggaacatcgg ctcgaacgac
     8641 ttcatcaggc gttccagcag gaagcgcagc ggcgccaggg cgcggatgag cagcagcatg
     8701 atcggcgcga accacacggc cagccgctcg gcatggagca ggccgatcct cttgggcccc
     8761 gcctcgccga agatcaccag cgcgaccgtg acgacgggaa tcgatatggc ctcgccgcgc
     8821 cccggaaagg cccggtcggc cagcaggaag ccgatggcgg cgatcccgac gttgacgatg
     8881 gtattgccga taagcacggt cgagaggatc tgggtcgggc gggagaggag ggtatgaatc
     8941 caggcgccga tggtcgggtt gcgctggctg acgcggcgca gggccaatgg attgagcgaa
     9001 aaaaagaccg tttcgctgct cgagaaaaac cccgagcaga tcatcaggcc aaccatcgcg
     9061 atgatcagta tgacggtcat gccgttgaac acgccgcggc cagcatagcg gatcggccgg
     9121 ccttttcgag cgtaaagaga gcccggcggc cgccggcctg attgagattc cggcgaaatg
     9181 ggcgatagta ggtcggggaa aacaacgagg gaacgctcga tgaaactctg gatagtcccg
     9241 gccctgctgc tggccgccgt cgcgaaggga gagcccgtga tgcatgaaaa cccgcaggaa
     9301 aataccgcca tcgccacgtt cggcggcggg tgctactggt gcatggaggc cgtgttccag
     9361 caggcgcccg gcgtgctgtc ggtggtctcc ggattcatgg gcgggacggt caggaatccg
     9421 acgtacgagc aggtctgcgc gggcggcacg ggccatgccg aggtgatcca ggtgcgctac
     9481 gaccccgcgc gcgtttcgta cgagcagctt ctcgagtggt tctggaaagc gcacgatccg
     9541 acgcaacgga accggcaggg gaacgacgtg ggcacgcagt accggtcagt gattttctac
     9601 cacgacgagg cgcagcggca ggcggccgag gcctcgcgcg cggcgctgga ccagtcgaaa
     9661 gttttcgcgc gcccggtcgt cacggaaatc gctgcggccg cggagttcta cccggccggg
     9721 aaataccacc aggactacta ccgccggaac ccgtcgcaac cctattgcca gttcgtcatc
     9781 cggcccaagc tgaacaagct cggactgaag gagtagccgc cggggcggac atcagaacag
     9841 ctcgggcgcc tgtcccctgg gtttcaggcc gaatttctcg taggcgcgct cggtcgccac
     9901 acggccctgg ggggtgcgct tgaggtagcc ctgctggatc aggaacggct cgtagacttc
     9961 ctcgatcgtg tccggttcct cgcccacgga cacggacagc gagtggacgc cgaccggtcc
    10021 tccgctgaac ttctggagca gggtctccag gatgcgcttg tccatttcgt ccaggccgtg
    10081 ctcgtcgata tccagcatgg cgagcgcccg gtccgcgacc tcgcgggtga tccggttccc
    10141 ggccttgacc tgcgcgtagt cgcgcaccca ggccagcagg ttgttcgcga tgcggggcgt
    10201 gccgcgggcg cgccccgcga tctcccgggc gccggccggc tcgacgtcca cgttcaggat
    10261 ccgggccgag cgcgtgacga tttcctgcag ggcggaggtg tcgtagtaat cgagccgcgc
    10321 ggccaggccg aaccgcgagc ggagcggcgc cgtcagcagc ccgctgcggg tggtggcgcc
    10381 caccagcgtg aagcggggga ggttgaggcg gacggaccgc gagtgcggcc cctggtcgat
    10441 gacgatatcg aggacgtagt cctccatggc ggcgtagaga tattcctcga cggacttctg
    10501 aaggcggtgg atttcgtcga tgaacaggaa gtcgcccttc tccaaattgg tcagaaggcc
    10561 ggcgaggtcg ccgggcttgt cgatcacggg accggaggtg accttgatat tcacgcccat
    10621 ggcgttggcc aggatatagg ccagcgtcgt cttgccgagg ccgggcgggc cggaaagcaa
    10681 cacgtggccc aggacatcgc cccgcccccg cgcggcctcg acaaacagtt ccagccgctc
    10741 cttcaccttc ggctggccga cgaagtcggc gaagcgggac ggacggaggc tgatatcgaa
    10801 ctccgtatcc gccttgttca gggtatccgt aacgaatcga tccgtcatag gcttctagct
    10861 cgctactata ggggttggaa ggaagccgcg gcaagaggaa tggaacccga cagcgacatg
    10921 aggggatgga gatcctgcta tttcgccatc aggggtgcgc cacactttgc aaagtgtggc
    10981 gcagccttca ggcaccggaa cttcacgcgg aagaacaagc tcccacgcca tctacgacgg
    11041 ccccattcag ccaaataatg cgttatttat tcggctattt aaatatattc gcgccggccc
    11101 aagggcgggg tctctcttat ttcagcccgt atcggccgtc tgaatccgcg ccacactttg
    11161 caaagtatgg cgcgccctcc tcgacggctt tccattggcc gggaaacgcg ctattcttct
    11221 ttcatggtca tcggcctcct gcaagccacg ttgagcattc cggaatccca ttcgctgaag
    11281 gacaagcgga tggtgctgcg cagcctcaag gaccgggcgc tcaacaagat gaacgtcagc
    11341 gtcgcggagg tggaccaaca ggacctctgg caggccgcga ctctcgcgtt tgtcaccgtg
    11401 gcggcggaaa aggatgtcgt ggaaaagcgg ctcgccgagg tcagcgagca cctgcggtcc
    11461 gacccccgcg cgatcctgct cgactacgag acgcacttgc tttaagcccg cgcgggtgca
    11521 gcaccagccc aaaatcctgc tccaggatgt ccaccaactc cgcgaccggc aggcccacga
    11581 cgttcgtgta cgagccccgg atcgcccgga tcatgaacga tccgacgccc tggatcgcat
    11641 acgcccccgc cttgtccatc ggctcgcccg tggcgatgta accggcgatc tcctcccggg
    11701 tcagccgctt gaattcaacg gcggtccgga ccgtgcggct gcgcctccgc acgacccgcc
    11761 caccggctgc gcgcgcgacg cagaggccgg agatcacggc gtgccaccgg ccgctcaacg
    11821 cctccagcat gcgccccgca tccgcccggc cccggggttt ctgcagaatc ctccgcccct
    11881 gcaccacgat ggtgtccgcc gcgatgatca cggccggccc gcggatgcgc gcggcgacct
    11941 cggcggcctt gtcctcggaa aggcggcgcg cataatgcgc ggggcgctcc ccccggttcg
    12001 gggtctcgtt aagttccggg ggaacaatgc ggaaccggaa gcccagcgat ttcagaaagg
    12061 cgcggcgacg cggcgacgcg ctggccagca cgagttgctc cttgcggttc atgcgccgca
    12121 aactatacca cgcccgtcgt tcagggccag ccggcttctt cgaaaaggcc tctgaacctg
    12181 aaggccaacg cccggtacgc cgagccgttg ggatggctgt cctcggagcc ggtggcatag
    12241 ctccgcttga gattcccgcc ggcatcggcc aggatcccgt agaagtcgaa tatccagcaa
    12301 ttctcgcggc cggacgcgaa gtcttcgaag cacagcttga aaaaagcgag ccgccccgcc
    12361 gtcctggcca cgccacgccc gggcgggacg ccggccctta ccgggcggaa acggcctccc
    12421 gcggccgagg cgccggccag gaggccaggt gaagatcgat cagcgcgagt tcggtcaggg
    12481 cccgattcag acaggcgggg cagtagccgt gcgtatagaa cgcgtcctcc gcctccagcc
    12541 tatccggcgc ttcccagcgg ccctcgcgcc ggacgcttcc gcacacgcag cattttccga
    12601 ccaccgtccg cggcataagt cactccccgt cctgctccat ccaaatgtac taacgccgtg
    12661 accgccggct tattgccccg cggtcccgtg cttttgtccc tcaccggggc gtcggcgttc
    12721 aacgaatatt cgcgcccagc gatcgctcgg taaaaaaaag ggaggccccc cggcctccct
    12781 cgcgctcccg tgcgcccggc ctgcgtcagc ggatcgtccc gccagcccgg acggtgtagt
    12841 cctgttcgaa gttgttgtcc cagaactcgc gccccaaagc gtcccggaag taaaccgcga
    12901 acttcgccgt cctgttgggc ttcgtcatgc gcgtcccgat gcgctgctcg aatcggcaca
    12961 cccgaacctg ggcgctgccg tacccgaaac cgtagctcgc cgcgctggaa accgggatgt
    13021 ccgtgaagtg cctccagttg tcggtggtca cccggatacc cgcgaccacg gagggcgccg
    13081 tgctttgcac cacgagggtg ccggccacat actgattgca caggcgcggg atcgccggca
    13141 ggccgctgac cgtgcggcag taattgctgg cgacgacgag ccccacgttg ccgcccacga
    13201 tgccatcgac ggctccgccc tgaacaatga cggagtaatc ggcgcccccg ttattgtccc
    13261 agtagtcacg cgtggtggtc atgcccgaga cggcgtaact ccaggtggcg cgaatcgcga
    13321 accgcatccg ggccctcgga acgcgcgcat aaaacagcat gtgcgtgccg tagtggccga
    13381 ccagcgacat gggcgtgtcg atccaagagc cgcccgcccc cccgtcatga tgcacgacgg
    13441 cgccccaact cccgcccagc ccgtccatgc tgtccacgtg gacatatata tcgctgtacg
    13501 accaactgac ccctccggcc gccccctcga tcggcgcggc gaataccagc ctcaagggag
    13561 actgcgccat cgcggacaag ctgctgccca gccacgccag caccaccacg actccccatg
    13621 ttatcgatct cttcatgttg tttcccttcc tgttaaacgg actcatccac cggccttcca
    13681 acgagttccg aatcatgtgg tgacggctcg aaacacctcc gtttctcgac ggacgccggg
    13741 gcttaccagt ccccccggac gggccagatc cctccgccga tgtctatttt tatgtgggcc
    13801 ccgaggtgtc cgccctcgat cccccacgcc cgggtcgtgt ccgcgcgata cgtgctgctg
    13861 gtccagaaga acgtctcgtg caggccgttg aagggatgtc cctccgggag aaaggatgca
    13921 ttgccgaagt cgatgaggct ccacgcctcg cgccggctgg gcagtcgcca gtccgcgtag
    13981 ccgccgaagg ccaggccgga acagtagctc acggcggagt gccagtcggc gacgggagtc
    14041 attccgagcg agcgcaccca catgcggccg aacaggttgt cgaacaccag gttggtgtcg
    14101 gtcatgacgg tgaatcgcgg gatcggccac ggcacgcccg gccgcaggtg cccgtcgtcg
    14161 cccaggacga tgctgtttgt ctggccggtc cggggcacgg gcattttacc cgtcgtgtgc
    14221 gcgcgcaccg cccaggcatg ggggctggta ttggtccggt tccagcgggc gatggtcccg
    14281 tcgcccaggc tcaccaccca ggcccggttg gtgtcggcgg cgtcgctcgt gctggtccag
    14341 tagttgagct gcacgttatt gaacggatgc ccggccggca gcgcgggcag gacgcgcttg
    14401 aggtcgagga ggctttccag ttcgcggacc gtcggcaggc gccaatcctc gtagtcgccg
    14461 ggcgcgacgc tccagtagac ggccgagacc atgaagtccc agttgttatg gccggagtcg
    14521 gcgttgcgcg cccacatgag gccggtcagg ttgtccacga caaggttggt gtccgcctgc
    14581 acggtgaacc gatccggcca ggtatggccc ggctccaggt cgccgtcgtc gccctccagg
    14641 tacgaattgg tctgcccggt ccgggcgaca caggtcgggt ccgagtattg ccagaattga
    14701 aagggctcgg atatcggcac cttgaggtgt tccatgctga cccagctgtt gtcgtccagc
    14761 cagagggccg actggaccgt caccgttccg cccacggcgt agttggtcca gacgaagccg
    14821 ccgtcgtgga tgaacaggtt gacgaactcg ctgccctcgt cgccgttgag gcggaagaag
    14881 cggtgatccg tgttccaatg acacagctcc tgcgcccgca ggcccggtcc gcccaggcca
    14941 gccagaagaa ataatgcgca ccaggttcgt ttcatggcct tctccttttc ggtctccgcc
    15001 gatgtggtcg cggcaggcgg ctgttttgtc tcgtcccgcc ggaataaatg agaagggggc
    15061 cgtggccggg ccccttactt tcgccgggcg cgtggtaaga tatacgggcc cgctccgggc
    15121 tggaccgcgt cgacgcatgc acgaactacg ccggcaacct ggtggtccag cacgaacgcg
    15181 atcctcgggt tggcgtccgc ggccaggtcg gccggttttt caggatcttt gagtccgagc
    15241 aagcacgttt cccgcctgga agaggcgaca aaggggctcc cggcaacgac cgtaacgggg
    15301 gaggccccgc aaggcgggat gcttccatga acagccggcg acaaggcgat aaccgtcggc
    15361 gggcaagctg gttgcgcttg acgcccggcg agcggacagg ccatgatgga cggatagggg
    15421 gcatgatgac cggaaacgca tttcgcattc cggggccgcg cgggaggagc ccggtcctgc
    15481 tcgcctggtc cggcgccgtc tttcttgcgg ccgccggact cgcgctcgcc ggtctgccgg
    15541 ccaacgggct ggtgctccac tactccttcg acgccgacaa cgggacgacc gtgcccgacg
    15601 agagcggcgg cggcaacctc gggctgatca cgaccaacct ggagtggacg gccttcggga
    15661 tctcggaggg ggcctaccga ttccacggca gcaacgagta catcaccgcc gggccgatgt
    15721 tcaactggac caacttctcg cagggcttca cgctatcctt ctggacgcga cagatcgagg
    15781 ccgagtttcg cggggtctat tatgcgggaa ccacgggcgg cagcggcgcg cagaccaacc
    15841 gtttcgacct cggctgcaac atcatcagca tgggcgacat cacgttccgc cttttcgacg
    15901 acgagggaga cgccattatc cgccagtacc cggccaccgg ggtcgacgac ggcgaatggc
    15961 accacgtggc cctgctggtc agcagcaatg ccaacgatat ccatctcatg cttgacggcg
    16021 cggatctcgc ggtgggcgcg ggctcgggca ccgtgttcca attgaacggc ggcttgaagt
    16081 tcccgtttgt gatcggcacg agcagcgacg ggggcggcgg cccgagccac accaactcgc
    16141 ccaacgggta cctggacgat ttccgcgtct acagccggcg cctcgcctcg aacgaggcgg
    16201 tgacgctctt ccggatgatg gaagacgagc ccaacgacac ggttccgcag gcgcgcccga
    16261 tcgccccggg ccagaccaag catcacacct tcaaggccct ggacgacgag gattgggagt
    16321 tcttctacac ttttacaaat tacacgaatt acgtgttttc caccaagaac accggcacga
    16381 acgtggatac ccagctcacc atctaccgca acctggacga cggcacgctg ggcctggtga
    16441 cgtccgtcaa caacacggcc atgggttcgg ggctcggcga gtcctggacg ctgaacaact
    16501 tcagcaacga cctctactac gtgaagatca gcgcgcccac cggcctctac ggcggcgggg
    16561 gaggcatggg cccggcgcgg cgggcggcgc gatccgacgg cgacgcgggc ttcggggagg
    16621 ggtcggagta ccagtccgag cttgtggcca cagcgacgcc gggcgtgcgg atcgtttacg
    16681 tggcggcgcc ggacaacggg cgccaggcgg gtgtgtgggc caaggtggat tccaccacgc
    16741 tctactttcc cgcgagaaac gaggggctgg cattcccggg cgtgtcggag gcgctccacc
    16801 tcgtggaggt gggcttcaag ccgggcgtcc gcccctggga gcacccgacc cgaccgggcc
    16861 agttggacaa cccgaattcc agctttggga acccgcactg ggtggacctg cgcggctcct
    16921 ccatgttcac gatctgcgtg ttccggtgcg aaagcctcaa ctaccagcag gccaccgtgc
    16981 gcgacggcta caccggggcg cacctgtccg acgcggccgt cacctgcatc tcggacagcg
    17041 ccccgttcac cggcacggaa acgcggcgcg gccacgccta cgaaacttcg taccagcagc
    17101 tgtggcgtac gctcgccgac gggtcgttcc ccaccaacct catgctctat cccaccacgt
    17161 gggatatccg gctgaaccgg gacggctacg taacccgcac catcaccgac gccgtcgccg
    17221 ccgtggggtg gggctacacc aacaacctgg gggacctgcg gctgtacccc gtggacgcca
    17281 acttcaacgg gatcgccgat gcctgggagc ttgctcactt cccgatcgaa gccttcccga
    17341 tcgatcccgc gggcgacctg gacgaggacg gcttgagcaa ctgggccgag tacatggcgg
    17401 acacggaccc gaccaacgac gccgaccggc tcgacctgtc gctcaccgcg gaaaacgaca
    17461 ccctccgcct ccgctggcag ggccggccct ggcggcgata ccggatcggc atgcaggcgg
    17521 tcgtggccga ggagggttgg gacagcgcga cggacaccgt aacccattcc gggacatccg
    17581 gttgggagca ggtctggtcg gatgtgaatt ggctggataa ctcgcagcgg gtgtaccggc
    17641 tggaggtgct gccgccatga agaagaggct gggaaagacg gcgcggtggg ccatgtccct
    17701 ggcgatggcc ctgccggccg cgggcaagga gttcaccatc gccacgccgc tgggcgagat
    17761 ccaggtggac gtgccgcacc tcacgccgca gggcgccgtg gtcaccccgg aggccgcgtc
    17821 ggtctccgtg ttccggccgc cgacgatctt ttccgcgccg ctgccgagcg gatccggcgc
    17881 gcgcgccctc ggcctcgccg gggccttcac ggcggtggcc gacgatgcga ccgccgcgtc
    17941 gtggaacccc ggcggcctgg tccagctcga gcgaccggag gcctcgttcg tctaccgcgc
    18001 gtcgggcgaa gacgacaccc accgcgtgga ctcggagacc atgagcgtcg ggtcggactc
    18061 gttccacagc agcggcctga actacttcag cggcgtcctg cccttcgagg ccggccaccg
    18121 gaacttcgtg ttctccatca actaccagga agcctacgac ttcgagcagc ggttcaccgc
    18181 cgactacacc gggcgctcct cgcgccggaa ctcggagacc gcgcaggcca gcgcctcctc
    18241 gaccttctcg gagacctatt ccctcgggaa cggcgaggtc acgctgacca gcgagatcac
    18301 cacggaaacg atccggaccc tcgaccaggc gctggcctcg gagctgctga cctcgctgga
    18361 tttcgaccag caggggctca tcgacgccgt cacgcccgcg ttcgcggtcg agctgacccc
    18421 gaaactgtcc gcgggcggcg ccgtgaatct ctacggcgac gacctgctcg gccgcaaccc
    18481 catccgctcc accagcacgg cgcgcttcgc cggcgccacg gacagccgcg tggacatcac
    18541 cgagcggcag accacgtccg gcgcctacta ctacgatgcg gtcgcgcacg tcccgccggt
    18601 cggcaatttc ccgggcttcg acgtgccgtt cccgcgggtg gagggcgtgt acgagccgtt
    18661 cacggacacg acggcctcgc gcgacgggga cctcctctat tacgagggca cgatcaccga
    18721 ggagaaccgg accgacgacc tgaccgggca gaacgccacg ctcggcgcgc tctggacggt
    18781 gtcccgcctg ctcgggctcg gcttcacggt ggacctgccc tggaccgccg aggcccggca
    18841 gcagcggacc gtccgcgaga cgatcacgac ctacgacgcc agccggacgc gcgtggtgga
    18901 cgtgagcgaa acgcgggaat ccgagacgcg ggacgtggag taccgcttcc cgctgtactg
    18961 ggccgccggc gccgtgcttc ggctcgcgga cgagttctac gtgaccatgg acgtcagcca
    19021 gaccctgtgg tcggacttca ccttccaggc cgagggcgag gagaaactta atccgctcga
    19081 cgggacgccg cacggccaga acccgctcga cgactgctgg tccgtgcgcg cggcgatgga
    19141 gtacatgctg ttcttctcca gggtcgagat tcccctgcgc gccggcgtgg gctgggagga
    19201 gcgccccgcg atcggcgagc cggacgagtt ctggaacttt tcgctcggca cgggaatctc
    19261 gctgggctcc gggccgcgcc gcgtgatcct ggacctcgct tacctctgct ccgtcggcga
    19321 gaacgcgctg ggcagcctcc tgcccggcac gggcatgacc tcggacgtcg aggagcacca
    19381 ggtctacgtc tcggggatcg tgcacttcta agacggcgtc gcgggagctc cgccctccag
    19441 gaggaatgga tggagggtcg agcgcccgcg agaccgccta aaaatataga caaaaacggg
    19501 cgggttgggt gtctgatcat gcggaggccc ggtgcagccg gaacaggaca gcgatggacg
    19561 gatacgcgac gcgctttccc gcgacgatcc cgtcgcgctg gattggatct gggaggcgta
    19621 tgccgctcgc ttgctcgcgt tcgcctcggc cattctctgc tcgcatcacg acgccgagga
    19681 aacgcttcag aatgttttcc tgaagatcgc ccgaaaccgg gaccgactgg ccgacgccgt
    19741 ctcgctcaag gcctacctgt tcacgatgac ccgcaacgag gcctgcacat tgatccggaa
    19801 gcggggccgc cgcgaggtgt ccgtggatcc ccgcgacttg tggctggtgc ccgcgggaaa
    19861 cccggagccc ggcctcccgg aggacgccga ggccgccgcg cagcgcctgg cgaccctgcc
    19921 cgtccggcag cgggacgtga tcgttctgaa ggtgttccgc gaaatgacgt tcgacgacat
    19981 cgccggcgcg ctggacatct ctccgaacac ggccgccagc cggtaccggt acggcatgga
    20041 caaactcaaa cgccgcatga atcgaggccg accatgaaaa cgatgcccga cgaatccatc
    20101 ctgaaatcct tccgcgtccc cgggcccgcg ccggaactcc gcgcgcgcat cctggcgtcc
    20161 gcccggctgg agtggaagaa acgggttctc ccctccgagt ggagccggtt gcggaacccg
    20221 ctccgcgcgc tcgcggcctc gatcgccgtc gccgtcctcg gacaatgggc caacggccgg
    20281 ctcgcggctt cgcccaacgc tagcgtcgcg cgcgtccgcg agccgctgcc ccgggaactg
    20341 acggagctgg cggaccgtcc cgccgtcttt cttatcccgg cggtgccggc ggccgacctt
    20401 tcttcgcgcc aggcgcaact gcgcgacctt ttggaaggcc cggccccctt gccggacatg
    20461 ccggcgcccc aaggacaaac ctacgataat agacaaacaa aaggcagccc tgcgtcttgt
    20521 tgttgaaaaa ggagacggtc catgaaaata gcggctgttc ggttcatcaa ggcgcttgtc
    20581 gcgctcctgg tcgccctgct cctgctccac acctccttgc tcatcctttc cgggctctct
    20641 ctccggaacg cctacaagga gttgcggcag gccgggcggc cgatcggcgc cgcggatatc
    20701 atcccgcccc cggtgccggc cggcgagaat gccgcgcctc tctacaccag cgcgttcgcc
    20761 cttctggacg ccaaaaccat cggcgaggaa cccctgttct cccgcctcgc ccaggccgcg
    20821 cgggactttt cgggcgagcc ggagtcggaa gaaaagcaag ccgcccttga acaactacta
    20881 gggcttgaag acgtggcccg cgccctggag atgattgaac aggcttccgc gcggccgaag
    20941 tgcaatttca acttgcagta tgaccagggg gccgcattgc tgtttcccca cgtgaacggg
    21001 atgctgaaga tcagccgcat cctggcggcc cgcacgaagc ttgaggcccg gcgccgcgaa
    21061 ggcgaccggg cttggtccgc cgttgaaacc aacctgcgca tggccgatgc gctccgggat
    21121 gaaccggcgc tgatcagcgc ccttgtccga atagcgatat ttcaggcggc cctgacctcg
    21181 gcccgcgagg ccgccgacct ggcggcgccc gacgatcaaa ccgccgcccg cctgatggcg
    21241 cggctggcca cagcggatga tccggcgccg tacgtgctcg cgatggacgg cgaacggatc
    21301 ctcatggggg aatgggcctt caaccacgtt cgcacacaga agttttttga ttgcttgggg
    21361 atggaagggg aggcctgtaa ctggaaaatg cagttgtata atctgctgat cggctatcgc
    21421 cccgcgcgcc aggcggacca ggccacctat ttgcgcgcca tgggcgaggt ggcgcagcgg
    21481 gcggcccggc cctactggga atcacccgtg gagccggaca gggagtggga tcgcgccatg
    21541 ccctggtatg gggccatttc caggctgatc ctgcccgccc tcggccaaag caggatcagg
    21601 gcggccacgc tccaggccga ggtgcggatc actcgcgcgg gccttgcgat gatccgttac
    21661 aagacggccc aaggccgtta cccggccacg ctggatgaac tcgttccggc tttcctggac
    21721 gagatcccca gggatccttt cacgggcgag ccgctggtct accgtccgga aggcgacggg
    21781 tttgtcctct acagcctcgg gcagaatcag caggacgacg gcggcacggc cgaatccccg
    21841 gacaatcgcg acaccaagac cttcgacatc gtatggcgcc gggcgcggtg atccctcaac
    21901 gacccggctt cccgcgcagc acgaacgccc cgtccgtctc gggcgcgagc gggccggcgc
    21961 gcacgacctg gccggtcagg cgctggaggc ggcgctggag cgcatgcacc aggtcggcca
    22021 cggacagcgc ccccccgggc ggattcgtca gcgcctccag cagcgcctgc gcgaagacgc
    22081 tgtgcgcgcc ctgggcgtcc ggcaccggcg ccaggtcgcc gctggcgatg acatatttcg
    22141 ccgggcgccc gtcgtcggga gcgttcccgg tcccatccca cagccgtcgg ccgaggagcg
    22201 agccgctgaa acacgcgtcg ctgatcacca gcacccggcg cggcgcggcg accagcaggc
    22261 ggtcggccag atcggcgtgg gcgatgcccg cctctttccg gacggcgccg aaggggatcc
    22321 agtatccttc gccgtcctca tcgaggacac cgtgcccggc gtagaaaagg atcacggtcg
    22381 cgcggtcgtc gagaaggcaa agctgatcga gggcgccaag gatggccgcg cgcgtggccg
    22441 ccgcgtcgaa cagccgccgg acctcgaagc catagcgttc ctccagcacg cgggccaccg
    22501 cctcggcgtc cgggcgcgcg gaggaaagcg cgggccaatt cccttcatag gcattgattc
    22561 caatgaccag ggcgacgccg gccgccggct cgcggcgctc gggcgcgcag gaacaaagag
    22621 ccgccaccaa cagggccgcg gcggctcgca ggactttacc gggcaaagac gacatcgcct
    22681 tcttgtacca cgtcccgggc cgccgaagcc accggttccg cgatcccgcg cgagcgctcg
    22741 gcgcgggtga cctgcagttc gaccggaacg ccgccggcca tgcgcacttc gccggcgccg
    22801 ggcacggcga cgatcgcccg ggccttcgcg cgcaccccgt cctcggcgcc cttgtccatg
    22861 acgatccgcc cgcgcgaggc ctggatgacc gccgcgcgga agagcgggaa acgccgttcg
    22921 atcttgctga cgaggcccgc gacctgcgcc gggagttcct cctccatcgc ctcgctgtag
    22981 acgtcctcga cgaagaggat ctggccgtcc gccgggtcca cgacgcgggc atagatgctg
    23041 cgcccggcgc cttcgcgggc gacggagcag accgcgagaa gttccgtgcc ggctttccgg
    23101 acgagggcaa ggtaaggggc caggtccacg ccggcctccg tttcggccgg ccccgcgggc
    23161 cagtcgagcg ccccgtccat gacgttgaag cgcacgggct cctcgtcgaa ggcctcgcgc
    23221 aaacggcggc acacggtttc tttcaggccc ggctcgcaca gggcgggcgg caggccgagg
    23281 gaaaggcggt actgccggtc cagagcgggc ggcgtctccc gcgtgaggac cagcttcttc
    23341 acgctccggt ttccggagcg atcgcgcgcg gccagctcga agcggttctc gcccggcgcg
    23401 aggggcaggc gctgcgaaaa ggtgtaggag agcgccccgc gctcgtcgga ggacagcagt
    23461 tccctgccgt tcagcagtac ggcggccagg ccgccccggt cgcccgcctg cccgtccagg
    23521 tagtaggcgt cctggtagac cgtcgcgacg agcgggccat tgatcaggag ttccggccgc
    23581 acgaggtcgc gctcctccgg ctcgttcgag gcccgcagga cgggctgtag cgaggcggat
    23641 tggacgggct cgttctcctg cagcgcgcgg accaggcgcg ccaggtccac gcgcaacgtg
    23701 ttgcccgcgc ggtccgtggc ctcaagccag gccggccggc cgggggcgat cgtgacatcc
    23761 acctccacgc ccggccggga agcggacggc ttcagcaggc gcgccgggtg cgagttcgtc
    23821 agcagggtgg cggattcgag ccggtcgtcg tcccggcata cgccgcgcgc ccgccagccg
    23881 ccgccctcgc gggccagcga gcggatgagc aactccggcc cgcgccagtc cgccatgatg
    23941 cggagtgtgg tcacggcctc gcggcccagc aggtcgcggg cctcgacgcg gatatcgttc
    24001 gcgcccgcct ccagcgcgac cgtccgggaa aagtcgagcg cgggctcggc gagttcgatg
    24061 aattcgggtt cgccgttgac ccgcagctcg cgcacgttac cctcgccttc cgcccggccc
    24121 gtcacggtga acgagcgcgc agccgtccag accacggcgt cctccacggt cagcatcacg
    24181 cgcggcggag cgagttttgc gccctgcaaa cgggcgcggc gcgcccggtt catgtagtgt
    24241 ttcgcgcgcc cgctgggctg ctgccggagc gagaggtcga ggtgcttttc cgcgcccgcg
    24301 acgtcgccca gcaggaagag gcagacgccc aactcgcggt gcgggtagta gccctcgagg
    24361 acatgcacgc cgtaggtccg cgcgcgccac ttgtcgcggg tgtagccgaa tttcgcgccg
    24421 ggccgcaggc cgaggcagcg ctcgaaatcc tcgcgggcct gttcggtttg tcctgctcga
    24481 agacgttccg taccgcgccg gtagtagttc caccagttat ggaagcgcag ctcgtccgcc
    24541 cgggtcccga catccggcgg aggactgtaa catccgccga gcagcaagcc acaggcgaga
    24601 agcagaaatc tgaccctatg aatcgtcatc gctttttctt caccacagag gacgcagagg
    24661 gtcgcggagg acggaggctc tgggatgatt tcctctgcgg acctctgcgc cctccgcggt
    24721 tcaattccgc gcttctgaat cggcgacgat ttcctcggcg acccggccgg cccaatcgcc
    24781 cgcgttgagc gaaaaatcct cggcattcgc gaacgactcc gaccacgtgc cggtcgccga
    24841 atcacccgga gagcgaaaag cctccagcgt ggccaggatt cggccgtccg ggaaggcccg
    24901 tatcgtggat cgcaccgacc gctgacggcc ggtccaccgg gcgatccagc ctgaaggccc
    24961 gtcgcgatcc gcgtacgtgg aggcgaccga ctgctgcagg ctttgggcaa aatcctggag
    25021 ttcggcctcg ctgtagaagg cctgctccac gccatccggc ccgcgcaccg ccccggcgtc
    25081 gaggcccggc gccatccaga ccaggccttc ggggctcatg gtgaccgcgc cgatgacaag
    25141 cgccagcgtc gcggccagcg ccacgagcgc cggtttccag aaggtcagga tacgactctc
    25201 gggcttcgcg gcgagcgtcc ggctccgctc ccagagctcg ccgatccgtt cttcaagggc
    25261 ggcttggctt tgctcggaga ctttcagcag gcgccggaac cgagcgtcca gttgcgcgcg
    25321 cgcttccagt tcgcggcgga gggttgcgtc ggtccggacg ctcgcccgga gcgcctcggc
    25381 ttccgccgta tccgcctggc cgtgaacgtg tttccacagt tccggccggc ctgactcgtt
    25441 ggtgttcatg tccgtctcat ccaccggtac tgtcgcctta cgcgggacat aaatcctgta
    25501 aaaaggaatt ttctgcactt tttccttcag ccgctccagg cagcgggcga gccggttgcc
    25561 gatggtgctg atcggctcgc cgctgcgggc ggccatgtcc ttgtaggaca ggtcctcgag
    25621 atagaaattc ttcagcgcct ggttgcacgg cgggcccatg tcctcaagca gttgccggag
    25681 cagggcggcg cgttcggcct cggcgatggc gtagacggga tcgaaatcct cgccggcggc
    25741 gtagtccagg tgccgcgttt cgccgtcgga cgtcggcacg tccatgggca ggtagcgttc
    25801 ccgctgcttg atctggcggc gcacctcgtc ggcgcaccgg tggatgcaga taccgcgcag
    25861 gaaaatccgg aacggctgct cgccgcggta attggcgatg gcccgcggga tttccgtgcg
    25921 gatgttctgc atcacgtctt cccgcgcggt ggagtcgaac ccccacttcg accagcagac
    25981 aacagagcgg atgaacggat cgaaccggaa gaagaactgc gtccaggcct cttgcgcgcc
    26041 agtccgcaac cgggccagga attcgtcctc catttcccac gaagaatcca tgccgggcca
    26101 caggataaac ccggggcggg ggacgacaca atccatgaat ggaaaggacg atcaccatga
    26161 aaatgcgcgc tctcttcgca ctcctcgcgg cctggggtct gtgtggtcac gccaccgtca
    26221 cctacgccac gacctattat gtccgggcgg gggcggccgg cggcggcacg tcctgggcgg
    26281 acgcgagggg caacctgcag ggcgcgatcg aagcctgcgc cgcctcgggc ggcggcgagg
    26341 tctgggtcgc ggcggggacg tattacccca cgttgacgcc gaacggcggc agcggtaccc
    26401 gcgaggtcca cttctcgctt cgcaatggtg tcacagtgta cggcgggttc ccggcgaccg
    26461 gctcgcccac gatggcgctg cgcgatccgg agaattatcc gaccatctgc tccggcgcga
    26521 tcggcgatcc ggacgacttc gacaacgtgt atcacctctt ctaccatccc accctcgccg
    26581 cgctcgacga gacggcggtc ctggacggat tccgtatcga ggacggccgc gcggacggcg
    26641 tgagcgacga cgagggcgcc gggatgtaca acgaatggtg ttctccgacg atccggaact
    26701 gcgtctatac ccgctgctac gcatcgaact gcggcggcgc gatgcacaac gcgtactcat
    26761 cgccgcgaat ccagggttgc gcgttcgacg acaatcgttc gggcgccgcg gccggggcta
    26821 tccggtttat ccgcggctcc ccggtcatcc aggattgtgt cttcacgaac aatgtggccg
    26881 agtcctacgg cggcgcactg caaaacatga acaccaccct gctggtcagc aattgtgtgt
    26941 tcgccaataa ccgcgcgacc ggcgagatgc cgatttccca tggcggcgcg atgtgcagcc
    27001 aggactcctt catcacggtg gtctcttcgc tgttcaagcg caacgcggcc gatctcggcg
    27061 gcgctttggc cgaattggcg ggcagcacac accactacgt ccgctgtacg tttatctaca
    27121 accatagtag ctccgacggc ggcgcgttct acggccttcg cgctccggac gtccggctta
    27181 cggagtgcgt cttgaaagac aacctggcgg actacggcaa tggcggggcc atctacgcca
    27241 atgacagctc cgtgacggcc atctcctgcg cgctggatgg aaatgaagcc tctgaagggg
    27301 gcggtctctt ttgcgatgaa ggcagcgaag cgctgatcgc caacagcatc gtgtccggca
    27361 acgtcgcgga ctgggccggc ggcagcggaa tacacatcca ttggtatgcc acgtgcacgt
    27421 tcgtcaacgg tgtcgtctac ggcaacagga cgtacgaaca cgacggcggc gccggagtct
    27481 atatctgcag cgccaaccaa tcgtacttct acaacagcat catccgcgga aatcgcgata
    27541 acgacgacga gtacgtcaat ttctccattt gcgacgagct cgcatggccg ggcgtcgcgt
    27601 tctgcaacgc cacgtacgac ggcggggtgt tccccggcag cgggaacagc tcggccgatg
    27661 tccagttcat ggacgtcggc gacccggacg gcgcggacga cctctggctg accgcggacg
    27721 acggctaccg cctgcgcgag gattccccgt gccgcgaggc gggaacggtc gaggcgctgc
    27781 ccgcggatct caccgacgtg gacggcgacg gggacacggc ggaagatatc ccgctggacg
    27841 cgatccggca cctccgcgtg cagggcgccc gcgtggacat gggcgcctac gagttcggct
    27901 cggcagtcca gccgacctac tcggtggatt tcgtcctggg cgcgcacggc ctgcggaccg
    27961 gcggcggcgc gctgcaccag gacgtggtcg aggatcacgc caccgtcgcg cccgaagtca
    28021 taggcatcga cggttggatg ttcacggggt gggacgccga cttcagccgc gtcacggccg
    28081 acctgaccgt caacgcgcag tacagcctga ccgcgcacac cgtccgcttc gacctcggcg
    28141 cgcacggggc ccgggtcggc ggcggcgaac tcgtgcagaa cgtcccgcac ggcgccgcgg
    28201 cggccgcgcc gctcatccgg gtgggcgtcg gctggtactt ccgggactgg gacacggcgt
    28261 tcgaccacgt gaccggcgac ctggtggtcc acgcgctgta ccaggaaatt tcgattcccg
    28321 gcgacatcgt gtacgtgaag gccggcgcga ccagcaacgg ttcgtcctgg gcgcaggcga
    28381 tgggcgacct gcaggcggcg atcgatttcc tcggcgaccg cggcgcgggc caggtctggg
    28441 tcgcgcaggg cacgtatgtt cccaactcgt ggtcgtgggg cgggtggacg gagcgcacgg
    28501 tccatttcgc cctgcgggag aacgtggaag tctatggcgg ctttcccgcg acgggcacgc
    28561 ccgggatggg cgaccgcgac tggatggctc acccgacgat ctgctcgggc gacctgggcg
    28621 cgctctacga ctacagcgac aacgcctatc atgtcttcca ggtgcgaggc gtcgaaaacg
    28681 cggtgctgga cggcttcgtg atcgaatggg gcaacgcgaa cgacctggaa agcccacaca
    28741 gcggcggtgg cgggatgcgc atcgagtacg gcgcgccgac catcgtgcgc tgcgcgatcg
    28801 gcgacaaccg ggcccgctac ggcggcggag tctactgcaa atacggccgc tcgccgacct
    28861 tcacggattg ccggttcagc gacaacgcgg ccatcctgaa gggtggcgga atgcggaacg
    28921 ccgccggcgc cagcccggac ctggatgcct gcgtgttcca cagcaacgag gccgagtggg
    28981 gcggcggcat ggccaacgac ctgtcctatc ccgacttgaa gcggtgccgg ttcgagcaga
    29041 atacggccac ggactggggc ggcgccctgc acaacgcggg cggatacgtg cggatgatca
    29101 acggcttgct tgtccagaac gaggccggcg acggcggggc cgtgttcaac tccgccggcc
    29161 tcttctgggc cttcaacacg gtctggcacg gcaaccgcgc ctcgttctcg ggcggcgcgc
    29221 tgtacaacga aatggacgcc gattcctacc tgtacagttc cacggtcacc agcaacaccg
    29281 ccggatccgg cggcggctcg ttcagctggg acgccgacca gcacgtgtac aacagcatcc
    29341 tgtggggcaa caccggcggc agcttcgtca acatggaggc cgcgcccgcg gtccgctaca
    29401 gcctcatcca gggcggatac gccggcggaa tcggggtatt gaatcaggac ccggtgttcg
    29461 cgaatcccct cgatccaaac ggcgcggaca acgagtggct cacggccgac gacgggctgc
    29521 aattgcaggc ggattcgatc gccatcaact tcggcgccgc ttcctacgtc acggccgaca
    29581 ccatggatct ggacggcgat gccgacctcg aggaagccat cccgctggat gccgcggaag
    29641 gcgcccgcac ggtgggcttc cgcccggacc tgggtgcgta cgagtacccg gccctggtca
    29701 cacacacggt cacgttcaac ctgggcgtcc acgggcaccg caccggcggc ggcgcgctgg
    29761 tccaggaaat cgagcggcac ggcgcggccg atgcgccggt ctttaccacc gatatcgggt
    29821 gggcgttcac gggctgggac cgggccttca cggacgtgac ggcccccctc gcggtgaacg
    29881 ccacctatga gttcgccttc gagaccggcg acatctgcta cgtccgccag ggcgcgaccg
    29941 gcaacggaac ctcctggggc gaggcgacgg gcgacctgca gggcgcgatc gacgcgatga
    30001 gttccgccgg cggcggccag gtctgggtcg cggcgggcgc ctacaagccg aactcgttcc
    30061 cgaacggcgg cagcggcgcg cgccagcagc atttctcgct ccgcaacggc gtcgcggtgc
    30121 tcggcggctt cccggcgacc ggcgatccgg gcttcggcga ccgcgatccg gacggccagc
    30181 tgacgatctg caccggcgac atcgggctgt cggacgagac ctccgacaac acctatcacg
    30241 tgttctacca tccggcgtcc gccgcgctgg cggcggccgc ggtgctcgac ggttttgtga
    30301 tccacggcgg ccgcgcggac ggcagcgcgc cgcacgacag cggcggcggc ctgtacaact
    30361 cctcctgctc cccgacgctg cgccactgcg tcttcctgtt caactacgcc cagaacggcg
    30421 gcgggctcta caacctttcc tcctcgccga aactgacgcg gtgccgcttc acgatgaaca
    30481 cggccgcgaa ccagggcggc ggaatgcgga actacacggc ctcgtcgccg tcgctggcga
    30541 attgcgtgtt caacggcaac gcggcgacgg acggcggcgc gatgtacagc cattccgcgg
    30601 ccccgatgct ggtgaactcc gtcttctccg gcaacacggc gtccgacgag ggcggcggga
    30661 tgtacaactc ggcggcggcg gcgatggtgt actgctcgac gttcgaaggc aacaccgcgg
    30721 ccgtccgcgg cggcggcatc tacaacacct cttcctcgcc gaccgttgaa aacgccatcc
    30781 tttggggcaa catcgcgccc gccggctcga acctgtacga cggagcgtct tcctcctcga
    30841 tggtcggcaa cagcacggtc ggcggcggat gggccgccgg gtacaacatc tccagcgccg
    30901 acccactgtt ccagaacgcg tcggatcccg atggggcgga caaccggtgg gccacgtcgg
    30961 atgacgggct ccgcctggct atctggtccc cggccctcaa cgcgggcggg aacggctcga
    31021 tcccgacaga cggccacgac ctcgacgacg acggcaacgt cgctgaaacg ctcccgatcg
    31081 acctcgccgg caaggcccgc gtaaccgacg acaccgtgga ccccggggcg tacgagttcg
    31141 aagtgcccgt gcacacggtg accttcgacc tcggcgcgca cggcatgcgt acgggcggcg
    31201 gcgaactggt tcagcgcgtc gaacacggcg cgtcggccag cgcgccggca ctcgacacgg
    31261 aggaaggttg ggcgttcacc ggctgggatc gggtgttcga tgccgttacc gcggacctgc
    31321 tcgtcacggc gcagtacgag ttccgcacct tcgatgtcac gttcgacctg ggcgcgcgcg
    31381 gcgcgcgggt cggcggcggc gaactcgcgc aggtggtcgg gtacggcagc gcggccacgg
    31441 ccccgcggct gaccgtggcg gatcactggt atttcaccgg ctggagcgcc gcgttcgacg
    31501 aggtcacgag cgacctggcc gtcgcggccg tgtactcgca gatcctgccc tcgggcggta
    31561 tttgctacgt caagcaaggc ggcacggaaa acggatcgtc ctgggacaag gccatgggcg
    31621 acctgcaggc ggcgatcaac gagatgcggc tggccggcga gggccaggtt tgggtcgcgg
    31681 ccgggaccta ccggccgaac tcgtggccga acggcggcac cggcgacgtc cgcgccatgc
    31741 acttcgcgct ggccaacgat gtcgaggtgt acggcgggtt ccccgccgcg ggcaatccgg
    31801 ggatggtcga tcgcgcgccg gcggccaaca tcaccctttg ctccggcgac atcggcgcgg
    31861 ccggcgtttc gaccgacaac gtctaccacg tcttccatca cggcttggag gccgcgctcg
    31921 agccgtcggc agtgctcgac ggattccacc tccgccacgg ccaggcgacg ggcgtattcc
    31981 cgcacaacga cggcggcggg atgctcaacc gggcgagttc gccgaccgtc cgcaactgcg
    32041 ccttctcgga taacgtcgcg gactggggcg gcggcatgca gaacatcaac tcctccagcc
    32101 cgctcgtcga ggattgctcg ttcatcaaca accagggctt ctggggcggg gccatgggaa
    32161 accagggcgg gtcgcacccg tccatttacg acagcgtgtt ccagggcaac agggccacgc
    32221 gcggcgcggc ggtatacagc ttcgaggcgt cgttccaggc gatcgcggac tgcgtgttcg
    32281 agcagaacca ggcgacggag aacggcggcg cgctctacct ggacggcgcc tgcgggatcg
    32341 tgtcccgctg cgagatccgc ggcaaccacg ccgggaacag cgggggcggc gtcgtgctgg
    32401 ccggcaactc ccggctcttc tgcagcctcg tcgcgggcaa ctcgtccacg atgtcgggcg
    32461 cgggcgtgta cctcgtgacc acgggccgga tcgagtactg caccatcgcg gccaatgtcg
    32521 ccggaaaaca cggcggcggg ctgcacatgg acgggcgggg cgtcgtcaca gccagcatca
    32581 tctacagcaa ctccgcggcg gcggagggcc ccaacggcca cgacgcttcc gccctcggca
    32641 cctacgatca ctgctgcacg acgccggagg tcgccggcgc ggggctggtg accgatccgc
    32701 ccgcattcgt cgccccggcc gccggcaact tccggctgtc ggccggatcg ccctgcgtgg
    32761 acgccgggcc ggccggcgcg gaggtcgtga cggatctcga cggtaggccc cggccgctgg
    32821 acggcgacgc cgacggcgcg agcgttctgg atatcggcgc gtatgagctt ctgaacgccg
    32881 acgccgacag cgacgctgac gagatgcgcg acgggtggga actggagcat gcgctcaatc
    32941 tccttgatcc ggccgacgcc gcggagaacc ccgacaagga tcccttcacc aaccgcgacg
    33001 aggccatcgc ggacacggat ccgttcgacc cgggctcgta cctgcgcatc acgcagctcc
    33061 tgttcatgcc cgagccgacg ctgttctact ggccggcgtc ccaccgccgg gcctacaccg
    33121 tgtgcgcgag cgccaatctc cggaccgcgg cgtggagcga cctgccgggc gcgacccgcg
    33181 tcgtgccgcc cgcttccggc ctgcagtcgt tccatctctt cggccgcgag acggagccac
    33241 gcggggaatg ctaccggctc cgggtctcgt acccgtgaca agaggcggcc cggcggaagc
    33301 gacgcccggc attgacagaa ttccccggat ggggggagaa tgacgggcgg aggtggccat
    33361 gaaagagcgc gggcggcttt ggctgtgtct cgtcctcgca gcggcgccca tgtcctccgg
    33421 cgcggagcgg ggcggccggc tatacccgat catgcagccg gactgggaga cacggcagga
    33481 atggttcgct tcccaccgcg ccgcgccgcg cgcggtcatt gacccgcagg ttcagtcgcg
    33541 gctcctcggc agcgtcagtc tgctctctca cgtccagtac acgccggcgg agcgcgatca
    33601 gtccagctgc gggaactgct gggcgtgggc gggcacgggc gtcatggagg tggacctgga
    33661 cgtccagcaa ggcgtcaagg accgcctgtc ggtgcagttc ttgagcacgt gctgcggaaa
    33721 gaccaagtgc ggctgccagg gcggctggct ggcggacgtg gccacctttt accagggcaa
    33781 ggggtacacg atcccgtggt cgaatcccaa cgggcaattc cagaaccagg gcggcgactg
    33841 cggatgcacg tgcgcatcca tcggcatctc gccgaactat cctctggcct ccatcagcta
    33901 cgccgccatc gaaacccagg gcatcgcctc gaatacggct atcgccaaca tcaagaatgt
    33961 tctaaatcag aacaaggccg tgtggtttgg tttctttttt ccgcgctcga gtgattggac
    34021 gcattttcag actttctgga acaaccaggc cgaatccgtc gtatatgtcc cgcccagcgg
    34081 cgtttgcggg gccacctggg actccggggg cggcggccac gcggtcctcg tggtcggcta
    34141 caacgacgac gatcccaatc ccgccaacca ctactggctc atcctgaact cgtggggcgc
    34201 gaccgcgggc cggccgaacg gcctgatgcg ctggacgatg tacttgaatt acaattgcca
    34261 gtcctatgac cgcagcacgt gggactacaa ctattactgg cagacgctga acatctcctg
    34321 ggccgccagc tccaggcccg tcgtcgcgac cagcaacgcc accgacgtga cctcctcgtc
    34381 cgccaccttg cgcggcgcgg tcaatcccag gggctcggcc acgacccgct ggttcgagta
    34441 cggcctgacg gcggcctacg gctcgagcac cgcggggcgc agcgccggct ccggcagcgc
    34501 gaacagcaac gtgagcgagg ccgtcagcgg cctcgcgccg gccaacacgt accacttccg
    34561 cctcgccgcc tccaacgccg ccggcgtcag ctacggcagc gacctgacgc tgacgacgct
    34621 ggcgggcggg gccacggcgc cggacgcgac caccgacgcc gcgtcgtcga tagggccgac
    34681 ttccgcggtg ctccatgcca cggtcaaccc gcgcggcagc agcacggcgg tctgcttcca
    34741 gtacgggccg acggaagcgt acgggttcac gacgcccacg cagcaagtcg gcgccggctc
    34801 cgcgccgacc tccgcggcgc gaacgatcag cggcctgtcc acggccgcgc tctaccacgc
    34861 ccgcgtgaaa gcctggaacg caggcggcac gacctatggc gcggaccgga cgttcagcac
    34921 gctgggggcc gccgtgacgc tgttttcgga gaacttcgag ttcgggggcg tgagcccgcc
    34981 gggctggacc cggcagtacg tccgggccaa cgaccacggc gcatccatcg actggtactt
    35041 ggcttacggc gacggcgcgc tctggcgcga catgcccgcg gcgccgcacg gcggcagttg
    35101 gaacgtgttc ttttatgacg aggactacaa cgcctggacg gccgtcatca gtcctcccat
    35161 ctccttcggg accggctatg cccagtccac gctcacgttc tggcactaca tggccgagga
    35221 ttactggtgg ggcgaccagg acgagctgct cgtctacatg ggcccgacca cgaacgggcc
    35281 gtggacgcgc ctggcgacct actccaacaa catcaccgcg tggacccagc agacgatcaa
    35341 cctgccgaat cccacgggca cggtctacct ctcgttcgac gcgatcagcc actggggtta
    35401 cggcgtggcc gtggacgacg tggaggtccg cgtcaaggcc tcggcggacc cggggccggt
    35461 catcgggcgc ggagcggcgg gcgacgggcg gctggtcctg cgctgggacg ccgcggccgg
    35521 cgagcagtac cgggtggaat acacgaccaa cctcgcggcg ggcgcctggc aggaagacgc
    35581 ggcggcgggc acgctggcgc cgtcggacaa cgaggtgtgg tacaccaaca ccccggccgc
    35641 gggcgcgacg aaccgcttct tccgcgtgcg ccggatcgcg ccgtaatggg ttgcggtccg
    35701 ccgcggcttc ccgtacactg ccggcatgac ggcgcctcga tgcatcctcg cggtcctggg
    35761 cctcgcgctc tgctccggct gcggcacgct accggacggg cggcgttggg gccatgaggc
    35821 caccctccgc ccgggctggc cgcgggttgg acagagcgcg tggcgggcgg cgatcgatcc
    35881 ccagacgtgg ggcccgctcg cggcggccgc gctgctgcag atcgacaacg cggaccacga
    35941 aatatcggac tgggcgatcg agcacgcccc gctcttcggc tcgccggagg aggccgaaga
    36001 cgccgtcggc tggaccggcc aggcgctgga cacggcctgg tacgcctcga tgctcctcgc
    36061 gcccggcggc gagacggcgg gggagtggac gctcaacaag ctgcgcggcg cggcggtgga
    36121 gttcggcgcc gtgggcgtcg cgggcttcac ggtggatacc ctgaaggagg aggttggccg
    36181 cacgcgcccg aaccaggaga acaaacgcag cttcccgtcc aaccacgcgc agcgatccgc
    36241 ggtggacgtc acgctcgccg cgcgcaacct ggacgccacg ccgatgccgc cggccgtccg
    36301 ctggacggcc aagacgggcc tctacggcgt gggcgccgtc tccgcgtgga gccgcgtgga
    36361 ggccgccggc cactatccgt ccgacgtcct cgccggctgg gcgatcgggc acttctgggg
    36421 cgcgttcctc cacgacgcct tcctgtggcg gggcgaaccg cccgtcctga tcgaactcgc
    36481 ccccgcgccg ggcggcgcgt cgctcatcct gcgcgcgcgg ttctgatcgc cggttgacag
    36541 gccggattcg agcaaggtat cttccgcttt ccgaccccga cggagaagca ctatggcgac
    36601 aaccgaagtg gaagtcctgc gtttcgacaa cctcgagcaa tacctgttcg tcaagaacgg
    36661 caattacctc tacaaggtgc cgtaccacgg cggcttcgcc gtgctcaagg tctactggtg
    36721 cagccgcggc tggttcgagc ggatctccaa gacgttcgac aacatcgtca tcgccggcca
    36781 gacgtcgttc atgccgcgcg cccgcctgcg tacggaacag caggccatgg cgatctggcg
    36841 cgaggccgga ttccgggtct tccagatcta cgagaacgtc cgcgtcgagg gcatcgagga
    36901 ggacggctac gcgctgtacg aatacgtccc cggccggcat ttccacaagc tcctcccgga
    36961 cgaatccgtg ccgcgcgagg agcggatccg gctcttccgc cagttcctcg tcgagtggca
    37021 ccggcggcac cgcctggcca tcgaccgccg cgagccccgg ctgatccacg agaacggcga
    37081 cctcaagcac gtgatggact acaacggcga actggtctgg ttcgacttcg agatgtgcta
    37141 ccgctcccgc tcacacgtgg aggatctcgt ggcgcgggaa atcctcgcct acgccaagtc
    37201 gatcggcgat ttcatgaagg gcgagctctt cgagctgttc ctgaacgaga tggtcgagca
    37261 ctatcccgac aaggacctgc tcggccacgc ctacaccgtc acgttccgca accgcaatcc
    37321 cgtgatccgc gtgggacgca acctggaatt cctgctccgc gagcgggcga agcggtggaa
    37381 ttccaagtac aacatggccc tgatgatccg ggaccgcctg gcacgcctcc atggacaagg
    37441 tgcagcgtct gcttgagcaa ctgcgcccgt ggcaggcgga gcggctcgac gggagcgccc
    37501 gcggctcggg cctgaacgcc gtctggaaga tccggctccc ggccggcccg gcgatcctga
    37561 agacgttttc gcggcggcgc ggccgcgcgc agacgctcct gaccgagatc aaccaccggc
    37621 tcggcggact cacctccttc acggcgcgcg gccgccggcg cacggaggcg gccatcctcg
    37681 ccctctggcg ggacgcggac ctcgacgcgc cgcgcctcct cgacgacccg ccggggctgg
    37741 ccctggccct gccgcacctg tgcatggaat tcgtggacgg cgagctgctt tcgcatcgcc
    37801 tcgcggacga aagccggccc ctggcggaac gggacgccct gctggtccga ttcctgcggg
    37861 cctggtccga ccgccatgcc ctggccgacg agcgccggta tccgcggctg atccaggagc
    37921 acggcagttt cgagcacgtg atggtcgcag gcgaccggct ggtcacgttt gacctcgaag
    37981 tgtccttcca gcggagccgc gacgtccggg cctgcatcgt ggcggagatc gtcggctacg
    38041 ttcgctcgct cttccggctg ctgccggcgc cgctggccgg gcattatctc ggcgtcgtgg
    38101 tccgggagta cccgcgccgc gagtacctgg aggaagtgcc gcgccggttg ctccgccatc
    38161 cgaatcccgt cctgcgcgcg atccatttcc tcgaccggcg cctgctgcgg aagccgggca
    38221 agtggcacaa gtaccgcgtg gccgagttat tgatgtccga actgaagatg cgctagactg
    38281 cccctcgatg cgcatcgttc atctcatcag ctattttcgc tggaccgagc gggtcgagcc
    38341 cgcggccgac ctggcgatcg cccagcagca gctcggccac gatgtccgct atctctgcgg
    38401 gcacaaccag ggcgcggcgc cggaggactg catccagggc cgcgccgcgc gcaagggcct
    38461 ggccttcgac gaccggttca cgctggacaa gcatttccgc ctcctgtccg cgctccggga
    38521 catcgcgggg ctgcggaaat acatggccga attcaagccc gacgtggtgc actgccacca
    38581 ggacaacgcc cacctgctgg ccgccttcgc gctgcgcggc ctggcccccc ggccgctgct
    38641 cgtgcggacg ttctacgagc cgggccggcc gtcgtcgccc gggcgctact ggcttttctg
    38701 ccaccgccgg acggacggcc tgatcgtcct caacgaggcg acgcgagccc aggcgcgcgg
    38761 gcgcttcggc cggcagcccg accgcgtcgc ggtcatcctg ccggggatcg acgtcgacgg
    38821 attcgccaac cgccgggacc tgggccgcct cgagcacgtg aacgtgccgc cgggcgcctt
    38881 cgtcgccggc atgatcacgc acatcgggaa ggtgcagcgc ctggacctgg cgctggaggc
    38941 cgtcgcggcg ctgaccccgc gccatccgga attgcggctg ctcatcgtgg gccgcggcaa
    39001 gccggatcgc tgggtccatg aacccgcccg gcggctcggc atcgccgacc gggtgatcct
    39061 gggcggctac tgccgctacg aggagctcgt gaaggcctac cacacgatgg acgcgctggt
    39121 gtatccccgc cacgggaccg acgaatcctg ccgcgccgtg cgcgaggcgc tgaccgccgg
    39181 cgtgccggtc gtcgcgccgg gaatcgccat catgccggac cttgtgaagg aaggcgaaac
    39241 gggtttcctt gccgatttcg acagcgcctc gcttgccaac gcgctggaaa agatgctccg
    39301 gctggatccc gccgcgcgcg cccggatgtc cgccgcggcc gtcgcggacg cccgcgcgcg
    39361 cttcagccgc gtgaagcagg ccgggaaagt cctcgagttc tacgaacggc tccgcgggct
    39421 gccgcggatg tagtgaggga aaacatcaag cgtgttccgg cgggcagcct tcgcctccgc
    39481 cgcgctacgg cgaaggctgg tgggcgtaac aggacttgaa cctgtgaccc cttccatgtc
    39541 aaggaagtgc tc
//
LOCUS       DAOOTK010000020        38734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_767_length_38734_cov_5.918069, whole genome shotgun sequence.
ACCESSION   DAOOTK010000020 DAOOTK010000000
VERSION     DAOOTK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 38734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38734
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_38734_cov_5.918069"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PK634_04095"
     CDS             <1..1290
                     /locus_tag="PK634_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96948.1"
                     /translation="LRYQTISNILPIVQKMLIASNASAAGASGVNTLVVRETADQLGK
                     IREVIGKIDKPRPQVFIEAKFIELNDQAIKDMGINWQALQGYTIGAAGMNWTLNDERI
                     RKKSTYSGLAETERRAQSDLMGQLYDQYGVSSPAGTEIGSGEAGFVSAPNGLDKKDDS
                     LGLAGRGTIDAISRAQTLDHSIEDSYDRTVSDVRAAVLSASDFSLVLSALKQNSGVSI
                     ISNPKIVVASGETASILVGSKRPNVSAVPLRSDSDGVVQFLYRLDGYLPIGVLVDVTP
                     IVNTESNITVRITPELSRAPRSEDEVIPQTGQRFPVMTISRVDTEFNVENNRTVAIGG
                     LSLTDDEEKIIKVPVLGDIPLIGKYLFTHTKTQKTQNEIVIFVTVALAQPDSMFERSG
                     IPSEGQLIHRHLTAREAQQVETGNGETKAGRKNGKKK"
     gene            complement(1318..2604)
                     /locus_tag="PK634_04100"
     CDS             complement(1318..2604)
                     /locus_tag="PK634_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOW96949.1"
                     /translation="MKKQKKEVIGVVGLGYVGLPLATAFATKFKVVGFDIKESRVKEL
                     KAGRDSTGEADLAHLKNPRLTFTSNPDALRRCTFIIVAVPTPIDESREPDLTPLESSS
                     TTVGKILRKGMMVVYESTVYPGVTEEYCLPILERESGLKLGQFDLGYSPERVNPGDKQ
                     HTVSGIVKVVSGHDARALDRCAAAYGAVIAAGIHRAPNIMTAEAAKVIENVQRDLNIA
                     LMNELSKIFARMGLNTNEVLAASATKWNFHRYHPGLVGGHCIGVDPYYLTHRALQLGY
                     HPEVILAGRRINDGMGNYVAELAIRELVNAGVLPRKARIWVLGMTFKENVPDFRNTRA
                     VDVVASLKAYGAQVYAWEPLVSAGQIKKEFGVDTLTFDRAKDLDAVVLINAHDAFKSI
                     ALSALRKKMRTRVLVDVKNFFPREQARKLGFRYVSL"
     gene            complement(2694..3710)
                     /gene="trpD"
                     /locus_tag="PK634_04105"
     CDS             complement(2694..3710)
                     /gene="trpD"
                     /locus_tag="PK634_04105"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOW96950.1"
                     /translation="MIREAIAKLVENRELTREEALAAMREIMAGEATPAQVAAYITAL
                     RLRGETPAVIAASAAAMREKFTAIPAPGEVVVDTCGTGGDGAHTFNISTAAAFVAAGA
                     GITVAKHGNRSVSSKCGSADVLAELGVKIDCAPEVMAGCLRDIGIAFLFAPVLHPAMK
                     HAIGPRREIGIRTIFNILGPLSNPAGARYGVLGVYHRDLVPVLAEAAASLGAQRLFVV
                     HGADGLDELTTTGDSFVAEVTRGAVKTYTLNPASFGIPRARPEDLRGGDAKENAALVR
                     SLLDGEKGPRRDIVLLNAAAAIVAGGRAADFAEGLQAAARSVDSGSARQKLAALARAS
                     NAGA"
     gene            complement(3727..4863)
                     /locus_tag="PK634_04110"
     CDS             complement(3727..4863)
                     /locus_tag="PK634_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOW96951.1"
                     /translation="MLHSRKEREKLEESCLAPYAVRSAASRGRLYPEPPHDVRAEFQR
                     DRDRVLYSKAFRRLEYKTQVFVNGTADHYRTRLTHTMEMTAVGRTLARALRANEDLVE
                     AIALAHDIGHSPFGHVGEEALDELMKQEGGFDHNLQSCRAVELIEHPYPDFPGLNLTW
                     EVRAGLRKHLAAQPGAALDGHPLGPRQFLEAQIADVADDIAYYAHDVDDGLEAGLISA
                     SELAGLEIWREAQGRLRVDPAADGAARATVRALLDLQVEDVLQTGADRVERFRPASPA
                     DVMSAPEKTVDFSPRMKALLTPLRTFLFERLYYNPAVSNDSQAAVRMMRRLFLHYIAH
                     PDVMGRKARARLASEGLWRTVCDYIAGMTDRYAMEEFERFKLNQ"
     gene            4936..5460
                     /locus_tag="PK634_04115"
     CDS             4936..5460
                     /locus_tag="PK634_04115"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOW96952.1"
                     /translation="MTSKWLYRGRILRLEVQDVQLANGRQAMREIVRHGPAVAVLARL
                     PDGRFLFVRQFRKPVEQALLEVVAGNREAGESAEDCARREMEEETGHRVTRLQALGFI
                     YPSPGYVDERIDLFFAEVEAEPGATRQDEDENVASVRLTRAEALAELKGRVVDSKTLA
                     AWLRSELAGLCGGG"
     gene            5477..6805
                     /locus_tag="PK634_04120"
     CDS             5477..6805
                     /locus_tag="PK634_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOW96953.1"
                     /translation="MAMGVKEGWQRLKNFLLHGIWEADLSALPPGRSLPIRIVRIGQL
                     VVKGFAEDDLAIHASGLTFVTLMSMVPMLAVIFALLKGFGFGQDRINQMMNWVSGMPP
                     EFQAFVDNMRDIANATNFAAMGWVGLVVVLFTSVLVLGTMELSFNRIWGVRVNRGVLR
                     QLANYISILVLIPLLVAGVGTAHASLRGGLLLLPKPFSLLAGSVLRLTTVVTTWLVFW
                     FIYVHLPNTKVKTLPALISSLLGAVCWLVWQKAYISLQVGVARYNAIYGTFASVPIFL
                     AWLYTSWVLILLGAELAFALQNSATYQIERAAENASSNARLIIGLSVLVRAAQALAGQ
                     AARFETAAFGREQRVPVRLLNDIVRLLVRTGFLAETTDKAGSYVLLRSADSIRVKEVV
                     DVVLQDGARPEALGLAHRDPAIERIIAQLETGVDRGLENMTLRECLHPAM"
     gene            6838..8778
                     /locus_tag="PK634_04125"
     CDS             6838..8778
                     /locus_tag="PK634_04125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOW96954.1"
                     /translation="MPPIDFEKDLNPEQRAAALAPDGPVLIIAAAGTGKTRALTYRVA
                     HLVERGVDPRRVLLLTFTNRAAREMLDRARLLVGEGVGGLWGGTFHHMANRMLRRHAG
                     ALGYQLDYTILDQDDARGLVREAADELGLLGKHFPKPDVLLSVFSLAANKEQAVADVA
                     GTWFKNHPIDINDVVRVHESYVKRKRGLQAMDFDDLLVNGLRLFREEPNVLRQYQEHF
                     LHILVDEYQDTNLIQAEWVDRLAEQRRNLLVVGDDFQSIYSWRGADFRNFLRFPKRYP
                     EATVFRLETNYRSVPGVLEVANACIAGNPEQFQKTLRAVRTEETRPVLVRLYGGEEQA
                     RYVVEALKRLRRGGVPMNRVAILYRAHFHAMELQMELAREKIPYVITSGVRFFEQAHV
                     KDACSLLRLLVNPGDELAFQRLMGMLPKVGQKTAIRIWEFLGKRCDLRDPAQAARLRD
                     KLPPAARDSWKQIEPLAAAYVEEHLDEDPGEVLHQFIEAFYDDYAVETFENAERRLED
                     LEALIDYTTKFESAEAFLSETALMTNLDAEADNVQGEPENALKLSTIHQAKGLEWDVV
                     FILWLAENMFPSARAMEDAGGLSEERRLFYVATTRARDQLFLCVPEMRRTRDGGAMYY
                     APSRFVRELPPGLVKEENVGFI"
     assembly_gap    8993..9002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9003..11471)
                     /locus_tag="PK634_04130"
     CDS             complement(<9003..11471)
                     /locus_tag="PK634_04130"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOW96955.1"
                     /translation="MKYLMLSLGLSVLILVLGPAAALAGPSLPVTKPPVADATILTGP
                     PRDAPAVTGQWFRSSSSGIETVRTHEFYDELLGGGSGSAAIAGYVSSITYDPAFNIIA
                     FSIVATINNDTSQTEFPWSGNNSHGESLILPGILSYVGPMYDTKLTAEFAVTDLGNLP
                     LIWNSPYRDRPPYIIADNEDQAAWYCWNPDYTNQERNPAGGFYVPTWDFGDIPQGQFS
                     TQQMDFSVQLPGISPLDTRYPVLQQSFSNQLDVLANRTTSLKVSTWIDEIALDVGQNP
                     EPPLRSSDVSVFHNAEETPDEQLDFGDAPDPSYPTSLATNGARHVIVPGIQMGLLIDA
                     ELDGQPNATATGDDNSNLDDEDGVTFLGSFIIGQSTDVVVQCSVAGTLAVWVDFNTNG
                     SWGDFGENVLGGVAVTQGLNYATINVPASASPGPTYARFRFTTLAVSMNPYGLVNDGE
                     VEDYQVTLQEPLEEGLDYGDAWDGGGVGVGYPTRFIQNGARHAFIPGVYLGARIDTEL
                     DGQPTINADGDDLNPPAGLDDEDGVVLPAVFVAGATATVTVVASVPGYLNAWIDWNSN
                     MSWLNPGEQVFVNQPLSAGINLLPVNVPLPPALVPGGPHSRWRFTTHAPVAPAFTGAE
                     TNGEVEDYEVRLEALDFGDTRAPYPTLLANDGARHRVPSAYWLGAISPDMDPDGMPSG
                     GADGDDLSNLDDEDGMVPSGAFVMGRTNRILVTASTNGYLDAWMDFNWDRDWADAGDQ
                     ICASYALAQGVNIVTVAVPAAIGVGQMGARLRFSSMGGLSSTGLASDGEVEDYWITIY
                     QPGPTTNIVVTNLIRSGVTARILWT"
     gene            11607..13211
                     /locus_tag="PK634_04135"
     CDS             11607..13211
                     /locus_tag="PK634_04135"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOW96956.1"
                     /translation="MANYIFITGGVVSSLGKGLTAASLGRLLISRGLSVRMLKVDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGQPTSKKSNFTAGRVYWEVLKKE
                     RRGDYLGGTVQMIPHITDEIKSRIRALDEAGVDVVLCEIGGTVGDIEGLTFLEAIRQI
                     GLEEGRERTMYIHLTYVPFIRAAGEVKTKPSQQSVARLREIGIVPDVLICRCEVPLSE
                     DVRKKLSLFCNVPLHAVIEEQDVRHTIYELPLVLAEQGLDELVLVRFGMARPPARLAP
                     WRKLVDGIVHPEGAVRIAVVGKYSELQDAYKSLFEAIHHGGFAHRRRAEILKIAAEEV
                     EDGQHRKTLRGADGILVPGGFGTRGIEGKIEAIRIARERKIPFLGICLGMQCAVIEFA
                     RNVGGLKGAHTTEIDKQAPHPVICLMEEQEAVAELGGTMRLGAWPCALKKGSQAAKAY
                     GGAAVSERHRHRYEFNNKYRAELEKKGLMLSGLSPDGRLVEAVELKNHPWFVAVQFHP
                     EFKSQPLAPHPLFKAFIGAACKKKSG"
     gene            13321..14490
                     /gene="carA"
                     /locus_tag="PK634_04140"
     CDS             13321..14490
                     /gene="carA"
                     /locus_tag="PK634_04140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOW96957.1"
                     /translation="MKALIALEDGTCFEGRAFAGPGEAHGELVFNTSMTGYQEILTDP
                     SYHGQIVTLTYPLIGNYGINAEDVESPRAQAAGLVVGECSRIPSNWRATKTLPDYLAE
                     HGILGIDHVDTRAVTLHIRSRGAMKCVMSTTDLDPARLVQKAKDSPGLVGRDICTEVS
                     TPAPYAWPGPHDGLKAFSTIREDGLKAYDSVFGAPPAAPRFRVAVMDCGIKLNQLRLL
                     ARLGCRCEVFSNSTSAAAILASKPDGFFISNGPGDPEGVPHTVAEIRKIVETGLPTFG
                     ICFGHQLLGLAFGGRTYKLKFGHRGGNQPVKNRLTGRVEITSQNHGFCVDKDSLDPAA
                     VETTHVNLNDGTSEGLRHRKLPVMAVQYHPEACPGPHDSTYLFRQFIGLMDSANR"
     gene            complement(14576..15646)
                     /locus_tag="PK634_04145"
     CDS             complement(14576..15646)
                     /locus_tag="PK634_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOW96958.1"
                     /translation="MKRIGLAAVCGWFWAWTALAAGPSAARQFGAEVADAVERVMPAV
                     VVVRTEATVYHPARDMFFGTLYGIPERLAGQGSGVIVRPDGYVLTSHHVIAGAEQVEV
                     VLDDGTKYPADLVGRDPLTDLAVLKIKAPAETKFTAIEPGDSDALRVGEFVIAVGSPF
                     SLDSSVTLGIVSQKGRSVGRLPYEDFIQTDASINPGNSGGPLVDVDGRMVGINAMIQT
                     GGPMVQGSIGIGFAVPVNLAMRVAEQLIERGEVERPWIGIQMGEVDDSPLARRSVRRE
                     AGVRVLEVFNNTPASRVGMAEGDLIIKVNGVAVATPRAVQREIFKRRAGETITLEVQR
                     EDKAMAFEIVTDTMPDFTQRAP"
     gene            15798..16826
                     /locus_tag="PK634_04150"
     CDS             15798..16826
                     /locus_tag="PK634_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW96959.1"
                     /translation="MSLITPVSAQPGTAGRPLSPSAPAVSVVVPFNAWNDWVMECLQG
                     LRRQTVPDFDVWLAPDALPPEPYRARLDAMGWGPRLHVEPTGPGNPSRKRNAALARSA
                     SPVAALVDSDAWPREDWLEKAVALLDDETAVVTGPNLTPPGDEWPRRVAGRVMESPLG
                     FGGGYIRHVPAPRQVVREMPTCNMVLRRLEGLLFREDLDTGEDMMYCSDVRARGKRVL
                     YDPEVVVFHHRRRLFRPFMRQFYAYGFHKGRLARAGSDITYLWQAFPALLVLYLAGWA
                     VSLLLPLPPALRWLAAAPGLLYAVAILAESLRRGRCAAERMAAPAAFAAAHAAYGTGY
                     LAGLLSRR"
     gene            16888..17757
                     /locus_tag="PK634_04155"
     CDS             16888..17757
                     /locus_tag="PK634_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW96960.1"
                     /translation="MKISGFTFIRNGHMLGFPFEESIRSVLPLVDEFIVAMGASQDDT
                     EERLRAIRDPKLRIIPTTWNERIRSDLGVKGFVYGQQKSIALFNCTGDWAFYLEGDEL
                     IHEDDIPKIRASMERNLDNPRVEALYFRYLHFYGNGQTLASSPAWYRREVRILRNTIP
                     AWAPKGLFFITLTSPKGGRYPRAADSDGAIYHYGWVRSEQQMIEKFDQVGKFWPGQKP
                     EIKLDEIDPQSLKPYTGTHPAAIRDWLPPATGLFQANPNHVLTRRERKNRWVARLERL
                     TRADFSKRHFTFA"
     gene            17817..21290
                     /locus_tag="PK634_04160"
     CDS             17817..21290
                     /locus_tag="PK634_04160"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOW96961.1"
                     /translation="MANSRCVGLIAVLVGGMEVNASARMWPDSSSRIVVFSDQLQSGL
                     TAAQRQFAATNLAGTQKMRRADIQALRAYNTNFLCLHYQLGVGNGPELFLDGDAWTSD
                     WSFVTTQTNWFMLNAQTQRVYHTTWNWYLMDCTYTSGLPRNGFPNYWITTCLARIRST
                     EGDGVFADSFTQDAYSFGQCSPTHPWIEDLGLCVSHWIPSLHSFGAACRAAFEADPGG
                     FLYLPNLGGLITSWDPTDFGLGHGGMIEGFCYWGTGNYFDRDDWRLQMDRALALVRTG
                     KTVICQSYPDTSAYSDRMFAIASYLLVKGARTYICLLTTGDVALEYYPEYTIALGGST
                     SPPPAATTSAWYGAWGVYRRGYTNGWTLVNPGGSAVNISNLGGTFYRVTASGGGAVDG
                     AGNYGGSLSTAPVTSLTVAAYSGEVLLFQSQAPSNTQPAALTTVHRSGQTFITWRERS
                     DLSGERYRVYRHAAPISTTNLAQAVPLYTLQKGSARFHANRYNVGGGGTWAPRYTDRL
                     VITNGGPALGVNTGLLVWTLATNDFGGATTGDAYYAVTLLVGATENTGDFSSANSIGP
                     VAEGVADPLPVLVTNYPAVPGGGAVRIYIQYMDLRNWNPSFHAPHARNGFYGQSGLDP
                     AVTGAIQYAYDYAVVEPSCVTSPAPAYISLHGWSGNTYGPVTSDPDAYDWCCYKIFPT
                     DMSETWFFGFARNCDYRTDADPAAGDTIDNFTERRVLRMLYDLIRQPPGPPVDTNRVY
                     VFGGSMGGSGALALALRYPHVFAAAYASQPMTDYSTQGDAGGTPWRDDIEWKWGSVVL
                     NLPIRSDAPGGWADHLKALDGTGIWTWQNHRRTASNAVARATVPLGIGHGTNDFVIEW
                     PTQGEPVYPAFNAGRRCWGGAVIDAGHSWMGWNGLPPTIAPDGSLAPFRRFTVRKDES
                     VPGLSNAEGDAPLPPAGPGGYNQELEWSAGWYDWDSAPTDATNRWQVSLRSLNGATQT
                     VDVTARRLQRFPVAAGTRVLWRNVLVANGTTSQVGVAAADGEGLITVPQVRVTPDGNR
                     LRLDADTGPDSDGDGIRDYWEDAFGFNSASTNDARSDADGDRSPNLDEYCAGTLPRDA
                     SSVFRITRYDDGSLAWLGVPGFRYALETTPALATAFAGHATVTAATFQSEAVVPFGDS
                     TSRFWRVRVDY"
     gene            complement(21284..22300)
                     /locus_tag="PK634_04165"
     CDS             complement(21284..22300)
                     /locus_tag="PK634_04165"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOW96962.1"
                     /translation="MKTMWRIGWLVMLAARLPAPARAEDAPDPAVAWEEAYYLEQGAG
                     DFAAAAERYERLANDERAEPAWRESARLRLAYCRLGGGRDADGRALLEALLDSPDARI
                     ADAAAAKLLDLAGEDTRAVAELLRSKAPAALARWEKARDAARVTLRGSIETWDGRRPS
                     PVHLRVEGRASEDARPVERPVWYADVDAEGRFTCPLPAGEYELVISTPVYERHRQRLV
                     LLPEMKDGFDLAVRLERIQLPDDVHQVYLLGDWLQDWTENLPMTRGSKGVWEVTQSLP
                     AGVHHYKFMVGHYRRWLCDARGAEFQDDARGGFNTVLRLAAGQDVVFRFDENDPRFAG
                     RPRQ"
     gene            complement(22297..22977)
                     /locus_tag="PK634_04170"
     CDS             complement(22297..22977)
                     /locus_tag="PK634_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW96963.1"
                     /translation="MKLRVLIVEDNVALRTGLELELRKREYEVVGAGDGATALRLARE
                     AKPDLVLLDLMLPRLNGYDVCRRLRELNMNVPILILTARTADEDELLSFQVGADDFVR
                     KPFKVDTLAARMQALLHRASAAPAAPQVAFGDCVIDVQAKTLRRGGEPVPLTPREYGL
                     LEYMTLNAGRVLSREQILDRVWGLDYEGTDRTVDRFVTVLRQKIEKDTRRPAHLLTVR
                     TFGYKFEP"
     gene            complement(22974..24947)
                     /locus_tag="PK634_04175"
     CDS             complement(22974..24947)
                     /locus_tag="PK634_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOW96964.1"
                     /translation="MSDLVLRHDARRPLLLLLVLVVVPCAALVLLAHQHVRSVRAAAA
                     RQYRETCEGELAALGLEARARLLQKLEDSAAAARARPREAPAEESGTDSCIETVFFLD
                     PAIPVLYRPYAAPPADDRPQLSAGHPARSRLLDARRAPAGEAERAISLYREVLASSPL
                     PGDVRASVLAEMAAREMAGGRTENALGLYEEWWTAASEASASRLPAALRALETAAAAG
                     LPARTSVWVARVMEEFGRPDRFPRRGEPACLAAFLQPWAPADAARLDGLARWFGELDR
                     LHPGPFQFTAWTGLPFQRAIPTGAAQAPDSLLLVDLERPLRDGRRIGWALSATGFVAR
                     VAGPVFRRFTAVHRGSLDWSAAGAPDRPSVLSQTLPAPLDLVAVRYDLDSSSLFLRLA
                     ASRTRYLTWTFGISVAWLLAGLGAVLYRVGRSMNLAKLQADMLDRVGHELRTPVAAIS
                     VLTESLENSSLDEPTRRQVAGLLRTESGRIQQLVERLLAYARGRRAARRLAFSEGRLD
                     DIVRQAARLFSVESGAGDALPVDVAAGNYAGRFDIQAVSQAILNLLDNAVKYSEPPPR
                     IEMALRRDGPQAVLTVTDHGIGMPPDVLRSIFKPYYRADTTLSASVGGIGVGLAIVQA
                     AVRAHGGRVGVRSTPGHGSVFEIRLPLGGGAEP"
     gene            25103..27499
                     /locus_tag="PK634_04180"
     CDS             25103..27499
                     /locus_tag="PK634_04180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOW96965.1"
                     /translation="MKKEKRLTVLGLTGAMLLGVATETFAIALTRGPYVQMGHFTNQA
                     TIVWYTDSSSDSGVDYGLTGSYGAFAGGNTGTRHEVTVAGLQPGTSYFYRVRSAGVNL
                     ATNRFKSAKAPGQPFRIAMWSDSHYGNTAGLAARMALYEPDLMLAAGDITDDGVLSEI
                     DNNAFSGFGGLMKQIPAYWTPGNHDTRDDFAACREVFTLPGDERNYWFEYADAQIVSL
                     NAEGLPGTAWLDGALAASAKPWKIVFFHEPARSPAAGHGEEESIRDQYVPVMERHGVQ
                     LALAGHNHFYWRSIPLNGVTHLITGRSGDRTRDLGDMPCYSQAGMNGTEAKSFAIADF
                     DGPFMQIRGIKENGDVIDDMVLDRECSFVLDGALDASAVQVAARAGGQTIWAAVAGRY
                     LYLATEDAGEGRDAYVFVATASGGLVASPWAKAGSVMAHDAFVADENDSRYSGWFTGA
                     GAPFNDLLAARSATPWCNGAVLEGVLDLQELYGEVPAVIRLAAAAYATADGGALAASA
                     QCPEGNGNGDIEAGEFVVVNTADLVQPFALDGAADHDGYLVADHNGMKLWAAVHGETL
                     YVATWAAGNGGGPNDHFIFVSDTPGTPGPAPWAKAGQVAFSEAAKPFLADEGADSWVA
                     WHNGGVSARQAAASVNSGQLEGTLNLVEVFGSVPETLYIAVAPYATADAGALYAPSQV
                     PEGDGNGNIDAGEFVAIPVAAIRDANLDGTLDILDPARGFVMSDGASGGNSAPYSVNW
                     SSQPGRRYRVQSTDDLKQPFANRTGELSADQGQFTMTYSDAAPPANGRRFYRVQLLDP
                     "
     gene            27569..28648
                     /locus_tag="PK634_04185"
     CDS             27569..28648
                     /locus_tag="PK634_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOW96966.1"
                     /translation="MIPLFGRGYDVLVNVTNICDARCVMCNIWKNQEGPTSWLRPEMM
                     RSVGPLTTVSFAGGEPFLHKEIVEIVRAVHGRNPRAKVVFSSNGFRTEPIVEKAGRIL
                     AFHKNVQVTISLDGVGEVHDRIRGIPGAWNKVNRTFDRLGEIGLKQRNFAFTITRDNH
                     TALPAVYAHARSKGAGLSLAVAQSSKFLNVEIPPLEFEAVRPHLEPIILAHLKSRNPM
                     DWMRAFFQFGVLRYLATRQRPIGCDALEHQFMIDQAGEVLCCHPLLLRAGNLGEADLP
                     KILSCPAANELKKDLRACHACWEVCTARSAIRANLFRVGLWAAGNKILAHLGLWSGKK
                     GSRLFPLTRERPPLNSQRPTPRESP"
     gene            28645..29310
                     /locus_tag="PK634_04190"
     CDS             28645..29310
                     /locus_tag="PK634_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOW96967.1"
                     /translation="MTTPSRHTSRVVSLQPLSDSGYLLTFERGGLEFEAGRLLTIHGR
                     VPTEDRSYTIASGEKDEHLQVLFRHIPTGQLTPQLAQKNPGDPIEFSGPFGEFVVRDP
                     SHPVIFIATGTGVAPCRAYLRTHPGLPLTLVHGVRVAQDLFFRGEFEKARYLPCVSRE
                     DGTGFHGRVTDFFKSFDFARDAHFHLCGANEMIFDMQVFLLNAGVDESHIFTEAYYYR
                     LHS"
     gene            complement(29292..29528)
                     /locus_tag="PK634_04195"
     CDS             complement(29292..29528)
                     /locus_tag="PK634_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96968.1"
                     /translation="MPWPRRPKGLFGPGPVPRLWPRRLEKALINHPRRIEFVQGLPTP
                     EQEEVLSILISRPASRRQGMEGRSPDRQNYECRR"
     gene            complement(29636..30925)
                     /locus_tag="PK634_04200"
     CDS             complement(29636..30925)
                     /locus_tag="PK634_04200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOW96969.1"
                     /translation="MRLNFESSTLPGGLRVATAVMPGLESVAMGIWAGVGSRCETGRQ
                     AGVCHFIEHMLFKGTSSRSAREISQAIEGRGGYFNGFTQEESTCYYARIAAEYQWEAL
                     DILFDMYRRPRLDPDDLEKERGVILEEIALYRDQPQQLVQEILGSLAWVDHPLGRSLV
                     GTEESVRRIRRRDIREFKQRHYVAANTLVALAGRVDHAACVERAARALGGLERRVPPA
                     PRRVDRAVRQRDVAVHAKEVEQPHVAMAFRLFGRRDPRRYALKLLSIILGENMSSRLF
                     QIVREKHGLAYSIQSSVQLFAETGLLCVQAGVDRRRAPRALDLILAETSRFRGTPVGG
                     RELRRAKDFARGQLRIGLESASNQMMWVGENLLCYNRLIQPSEVLDRIEAVTAEDVLE
                     VARRVLDPRRLSIAMVSPGDATRHTDMIRHKIARLEK"
     gene            complement(30916..31641)
                     /gene="hisA"
                     /locus_tag="PK634_04205"
     CDS             complement(30916..31641)
                     /gene="hisA"
                     /locus_tag="PK634_04205"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOW96970.1"
                     /translation="MIILPAIDLKGGRCVRLRQGRADDAVVYSDDPVEMARRWRDEGA
                     EWLHVVDLDGAFQGRPAHLSVLRAIARAIAIPVEFGGGLRTDADIDAALDAGARRAIL
                     GTRACAEPEQVKSLAARFGARLAVGIDARDGRVQVKGWTETTPMTATDLARRMEGMGV
                     RCLIYTDTSRDGMLAGVNASAMDALCSAVACDVIASGGVTSAEDVRALRALRRPNLAG
                     AIVGKALYEGRATLAVLREAACA"
     gene            complement(31638..32258)
                     /gene="hisH"
                     /locus_tag="PK634_04210"
     CDS             complement(31638..32258)
                     /gene="hisH"
                     /locus_tag="PK634_04210"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOW96971.1"
                     /translation="MIGIIDYGMGNLGSVSNACRFLGLPFSILARPEEMKACRAVILP
                     GVGAFGDCMDHLVRNGYVEAVREWIGASRPFLGICLGLQVLYEGSEESPGVPGLGVLP
                     GRVRRFNLPPEWKVPQIGWNRVRQVRRDCPLFAEVPDGAYFYFVHSYYAEKTNTAVDA
                     GATEYGLEYTSAVWRDNVLAVQFHPEKSQKNGLQMLRNFGRWADRR"
     gene            complement(32313..32903)
                     /gene="hisB"
                     /locus_tag="PK634_04215"
     CDS             complement(32313..32903)
                     /gene="hisB"
                     /locus_tag="PK634_04215"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOW96972.1"
                     /translation="MKKRAAEIKRETRETSIRAKLALDGAGEARVRTGIPFLNHMLEL
                     FARHSLCNLELRAKGDLAVDYHHTVEDVGLVLGEALNRALGDRKGIVRYGWAVVPMDE
                     SLSRVAMDLGGRPYLVYEVEPRKRKIRDFDLGLIEEFFRAFTVQGRMNLHIKTMYGKE
                     PHHIYESVFKAVARALGMACARDPRVRGVPSSKGRI"
     gene            complement(32996..35023)
                     /locus_tag="PK634_04220"
     CDS             complement(32996..35023)
                     /locus_tag="PK634_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOW96973.1"
                     /translation="MKFFSNFKLLIVLMVLGMLLATAVAFMLFERAEVTRAVLASGDE
                     SALNVLRVVNLNIASQYKDLEAFRQYATDRHQQQLRNLTGVVVSHIDTYHKLYEQGAM
                     TEPEARRRALETVQDFRYGHDDYFIIYDTNHCAVSHPDPNVRGRNMSATLDSRGRPIL
                     ATMESEIFQTGEWSCTIAWPRRGETNPVPKLLYFQPYTNWGWYVGTGVYIDDIDADAA
                     RRMGEIMEVLKTTFSQVKVAETGYFFLFDEYGRFLIHPSMTSIDTAMAGSLFTNFVRA
                     AENPGVPYQYQWDKPGDLGRHVYTKYAHVEKFEPLDWYLASSVYKDEMEKPARRIIKR
                     QARYVGVIVLFGMLGAYFLVSRLTRPLARLTHHADRLQASDFAMPEDEAKDILAITFP
                     DEVSRLAATMSSMEQRLKEYLKNLKETIAARERMQSELRIAHEIQMNMLPRHAAEIEA
                     RREIDLVAELVPARDVGGDLYDYFFVDDDRLALVVGDVSDKGVPASLFMSRSIALLRH
                     IAIKESVEPDAIFKMVNRDLLQGNDLCMFLTAWMGILDTRTGELVYTNAGHLPPFVMP
                     VIGGCRLLDLPPGPPLGVIEEADYQARRVRLAVGDGLVVFTDGVTEATDRNVQLYGYA
                     RLEALLSRCRACDAAARIVQTIMHGVKDFVQDMPQSDDIAILCVRRTSSEA"
     gene            complement(35185..35892)
                     /locus_tag="PK634_04225"
     CDS             complement(35185..35892)
                     /locus_tag="PK634_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOW96974.1"
                     /translation="MHLAGYRLHADRLPSRERYPFCLPALLHTPELDLNHGVVLFVGE
                     NGSGKSTLLRALCRNAGIFIWGGFADLSADEEAFLRAVELRWARGPMPGSFFSSETFF
                     NFAQVADHDSAMHEFFGPRGFGAQSHGQSLMAYFSARYRLPGLYFLDEPETALTPRGQ
                     LDLLRLLQAMSRDGHAQFIIATHSPILLACPGARLYGFDGPAIRPVAYRETEHYRFYR
                     DFMENPLPFLGPPRENG"
     gene            complement(35894..37135)
                     /locus_tag="PK634_04230"
     CDS             complement(35894..37135)
                     /locus_tag="PK634_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96975.1"
                     /translation="MPNLEEQRVTGPGEGRTLFVPPMDPCGARLLAAVFRSVGYEAYV
                     LEENAETLAIGHKHTSGGECVPCPLTVGALVKAMQDRKLPPERVIFFMPTACGPCRFG
                     QYAKLDSIIFEKQGWGGIRILSPSAENAYAGLDSGARMRLWHAAVVGDILRKLGMKVR
                     PYEQRPGETDVTLEQGLRRLEKAFEQKDVGPVTPLLAGLVDEVARVARRPEKRPLVGV
                     VGEIYVRCDPFINGQVCRRIEELGGEAWLAPVSEWILYTNYLQTLVASSTSGLRARLE
                     NLRTWLERNLFFEKWEHHYYEVAEPVLHDRKEHSIAEVIQAGAKYLPWQFEGEAILTL
                     GRAVLFVRRDGARAVVNASPMFCMPGTITTSIFPQVERETGAPMVCLFYDGSGDPNQA
                     LVPVMHYLREPGPADVPQGRP"
     gene            complement(37153..>38734)
                     /locus_tag="PK634_04235"
     CDS             complement(37153..>38734)
                     /locus_tag="PK634_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOW96976.1"
                     /translation="QPCPRTSDRCTVYMERDLDLLMARGWSKDQIAAAVLHSVRDNYL
                     NKVVGGLHIGDHVYFQGATARNKALVAAFETELQKPIEVSPFCHLTGSLGMSLLARER
                     VPEAERSARFKGLGFADEKVTVDREVCDLCHNLCNLSIIRAGREVVAWGLKCGRDYGE
                     KRMRVRDLSAYEFWKRRAAAWSDGAGPAAPAPGPRVGLLRGLGTYGYYPFWRTFLREL
                     GAAVVYSPPSSEKILHRGGEITTAEYCAPVVMSHGHARALAEDPGVDYLFVPYMLREP
                     TPEGFTDAHFCCYVQAHPGVLRSIEGMNLGPRLLAPVVDLGRPEEEQVAAIGLAVGEP
                     LKADAAAVRRALRAAKDAQAAFRRHCIELGREALERIEKENALGLVCFGRPYNTTDPG
                     LTLDLPRKVAQMGYHVLYQDMLPFDTADILPGHRNMYWHYGQKILAAAECVARSPRLF
                     GVYFTNFMCGPDSYILTYFKEIMGRAGKPYLCLQFDGHGADAGYLTRIEAALESFHSW
                     SKIPRPAAKEVNSESFSS"
BASE COUNT         6179 a        13558 c        12939 g         6048 t
ORIGIN      
        1 ctgcgctacc agacgatcag caacatccta cccatcgtcc agaagatgct catcgccagc
       61 aacgcctcgg cggcgggcgc gtcgggcgtc aacacgctgg tcgtgcgcga aaccgccgac
      121 cagctcggga agatccgcga ggtcatcggc aagatcgaca agccccggcc ccaggtcttc
      181 atcgaggcca agttcatcga gctcaacgac caggccatca aggacatggg catcaactgg
      241 caggcgctgc agggctatac catcggcgcc gccggcatga actggacgct gaacgacgag
      301 cgtatccgca agaagagcac gtacagcggg ctggcggaga cggagcggag ggcccaatcg
      361 gacctcatgg gacagctgta tgatcaatat ggcgtgtcca gtccggccgg caccgagatc
      421 ggctccggcg aggccggctt cgtcagcgcg cccaacggcc tggacaagaa agacgactcg
      481 ctgggcctgg cgggccgggg gaccatcgac gccatttcgc gcgcgcagac gctcgatcac
      541 tccatcgagg acagttacga ccggacggtc tccgacgtcc gtgccgccgt gctctccgcg
      601 agcgatttct ccctcgtcct gagcgccttg aagcagaaca gcggcgtcag catcatctcc
      661 aaccccaaga tcgtcgtcgc cagcggcgag acggcctcca tcctcgtcgg cagcaagagg
      721 cccaacgtat cggcggtgcc gctgcggtcg gattccgacg gggtggtcca gttcctgtac
      781 cgcctcgacg ggtacctgcc catcggagtg ctggtggacg taaccccgat cgtcaacacc
      841 gaaagcaaca tcaccgtgcg catcaccccc gagctttccc gcgcgccgcg ctcggaggac
      901 gaggtcatcc cccagaccgg ccagcggttc ccggtgatga ccatcagccg cgtggacacc
      961 gagttcaacg tggaaaacaa ccggaccgtc gccatcggcg gcctctccct gaccgacgac
     1021 gaggagaaaa tcatcaaggt gccggtcctg ggcgacatcc cgctgatcgg gaagtacctg
     1081 ttcacccaca ccaagacgca gaagacgcag aacgagatcg tgatcttcgt caccgtggca
     1141 ttggcccagc cggacagcat gttcgagcgg tcgggcatcc cgtcggaggg ccagctgatc
     1201 caccgccacc tgacggcccg cgaggcgcag caggtcgaaa cgggcaacgg cgaaacgaag
     1261 gccggccgca agaacggcaa gaagaaatag cctgccccgg tagcgccgcg cggcgcgtta
     1321 caggctgacg taccggaagc ccagcttccg cgcctgctcg cgcgggaaga agttcttcac
     1381 gtccaccagc acgcgggtgc gcatcttctt ccgcaaggcc gacagggcga tggacttgaa
     1441 cgcgtcgtgg gcattgatca gcaccacggc gtccaggtcc ttcgcccggt cgaaggtcag
     1501 cgtgtccacg ccgaattcct tcttgatctg gccggcgctc acgaggggct cccaggcgta
     1561 cacctgcgcc ccgtacgcct tcaggctggc gaccacgtcg acggcgcgcg tgttgcggaa
     1621 gtcggggacg ttttccttga acgtcatgcc gagcacccat atccgcgcct tccgcggcag
     1681 cacgcccgcg ttcaccagct cccggatggc cagctcggcg acgtagttgc ccatgccgtc
     1741 gttgatccgg cggccggcga ggatgacctc gggatgatag cccagctgga gcgcgcggtg
     1801 ggtcaggtag tagggatcga cgccgatgca gtgcccgccg accagtcccg gatggtagcg
     1861 gtggaagttc cacttggtcg cggaggcggc cagcacctcg ttcgtgttca ggcccatccg
     1921 cgcgaagatc ttggaaagct cgttcatcag cgcgatgttc aggtcgcgct ggacgttctc
     1981 gatcaccttg gccgcctcgg cggtcatgat gttcggcgcc cggtggatgc ccgccgcgat
     2041 caccgcgccg tacgccgccg cgcaccggtc cagggcgcgc gcgtcgtgcc cgctgacgac
     2101 ctttacgatg ccggagaccg tgtgctgctt gtcgcccgga ttcacgcgct ccggcgagta
     2161 gcccaggtcg aactggccca gcttcaggcc ggattcccgc tccaggatcg gcaggcagta
     2221 ctcctccgtc acgcccgggt acacggtgct ttcgtacacc accatcatgc ccttcctgag
     2281 gatcttcccc acggtggtcg aggaggattc gagcggcgtc aggtcgggct cgcgcgactc
     2341 gtcgatcggc gtcggcacgg ccacgatgat gaacgtgcag cggcgcaggg cgtcggggtt
     2401 cgaggtgaag gtcaggcgcg ggttcttcag gtgcgcgagg tcagcctcgc ccgtcgaatc
     2461 gcggccggcc ttgagttcct tgacgcggga ttccttaatg tcgaacccga ccaccttgaa
     2521 cttcgtcgcg aaggccgtag ccagcgggag ccccacgtat cccaacccga cgacgccgat
     2581 gacttccttc ttctgcttct tcatggtgcc cctgccggtt ggaagttgaa ttgggcgctt
     2641 tttataggcg gtttgcccga aaaagcgaag ccaaaagagc aggatcggcg atctcacgcc
     2701 ccggcgttcg aggcccgggc cagcgcggcc agcttctgcc gggccgaccc gctgtccacc
     2761 gagcgggccg ccgcctggag tccctcggcg aagtccgccg cccggccccc ggcgacgatc
     2821 gcggccgctg cgttgagcag cacgatgtcg cggcggggcc ccttctcccc gtccagcagc
     2881 gaccggacca gggccgcgtt ctccttcgca tcgccgccgc gcaggtcctc cggccgggcc
     2941 cgcgggatgc caaaggacgc ggggttcagc gtgtaggtct tcaccgcccc gcgggtgacc
     3001 tccgcgacga acgaatcacc ggtcgtggtc agctcgtcca gcccgtccgc gccatggacg
     3061 acgaacaggc gctgcgcgcc caggctcgcg gcggcctccg ccagtacggg gacgagatcc
     3121 cggtggtaca cgcccagcac gccgtaccgc gcgcccgccg ggttcgagag cgggccgagg
     3181 atgttgaaga tggtccggat cccgatctcg cggcgcggcc cgatcgcgtg cttcatcgcc
     3241 gggtgcagca ccggcgcgaa caggaaggcg atgccgatat cgcgcaggca cccggccatg
     3301 acctccgggg cgcagtcgat cttgacgccc agctcggcca gcacgtcggc gctcccgcac
     3361 ttgctcgaca cgctgcggtt gccgtgcttg gccacggtga tccccgcccc ggccgcgacg
     3421 aaggcggcgg cggtcgagat gttgaacgtg tgcgccccgt cgccgccggt cccgcacgtg
     3481 tccaccacga cctcgccggg cgccgggatg gccgtgaact tctcccgcat cgccgccgcg
     3541 ctggccgcga tgacggccgg ggtctcgccg cgcaggcgca gggcggtgat gtacgcggcg
     3601 acctgcgccg gcgtggcctc gcccgccatg atctcccgca tcgcggcgag cgcttcctcg
     3661 cgggtcagct cccggttctc caccagcttc gcaatcgctt cgcgtatcat ggtcgggccc
     3721 tcccgtttac tggttcagct tgaaccgctc gaactcctcc atcgcgtagc ggtcggtcat
     3781 ccccgcgatg taatcgcaca ccgtccgcca caatccttcc gacgccagcc gcgcgcgcgc
     3841 cttgcggccc atcacgtccg ggtgcgcgat gtagtgcaga aacaggcgcc gcatcatccg
     3901 gacggcggcc tggctgtcgt tgctgacggc gggattataa tacaggcgct cgaacaggaa
     3961 cgtccgcagc ggcgtcagca gggccttcat ccgcgggctg aaatcgacgg tcttctccgg
     4021 cgcgctcatc acgtccgcgg gcgaggcggg ccggaaacgc tccacccgat cggcgccggt
     4081 ctggaggacg tcctcgacct gcaggtcgag cagcgcccga acggtcgccc gggcggcgcc
     4141 atctgccgcg ggatccacgc ggaggcggcc ctgcgcctcc cgccagatct ccagcccggc
     4201 cagctcggac gccgagatca gcccggcctc caggccgtcg tccacgtcgt gcgcgtaata
     4261 ggcgatgtcg tccgcgacgt cggcgatctg cgcctcgagg aactggcgcg ggccgagcgg
     4321 gtgtccgtcc agcgccgcgc ccggctgggc ggcgaggtgc ttgcgcaggc cggcccggac
     4381 ctcccacgtc aggttcaggc cggggaaatc cgggtaggga tgctcgatga gctcgacggc
     4441 gcggcagctc tggaggttgt ggtcgaaccc gccctcctgc ttcatcagtt cgtccagcgc
     4501 ctcctccccc acgtggccga aggggctgtg gccgatatcg tgggccaggg caatggcctc
     4561 gaccaggtcc tcgttcgccc gcagcgcccg cgcgagggtc cggccgacgg cggtcatttc
     4621 catcgtgtgg gtcaggcggg tgcggtagtg gtcggcggtt ccgttgacga acacctgggt
     4681 cttgtactcc aggcggcgga aggccttgga gtagagcacg cggtcgcggt cgcgctggaa
     4741 ctccgcgcgc acgtcgtgcg ggggctcggg atagaggcgg ccccggctcg cggcgctgcg
     4801 cacggcgtag ggggccaggc aggactcctc cagtttttct ctttcttttc tcgaatggag
     4861 catcggttgt atattaggct gatccggtcg attggcaacg acggaggaag gattcgccgc
     4921 gtggaagaga aaaccttgac atccaaatgg ctgtaccggg gccggatcct gaggctggag
     4981 gtgcaggatg tccagctggc aaacgggcgc caggccatgc gcgagatcgt gcggcacggg
     5041 cccgcagtgg ccgtgctggc ccgcctgccg gacggccggt tcctcttcgt gcgccagttc
     5101 cgcaagccgg tcgagcaggc gctgctggaa gtcgtcgcgg gcaaccgcga ggcgggcgag
     5161 tccgcggagg actgcgcccg gcgggagatg gaggaggaga cgggccaccg cgtgacgcgg
     5221 ctgcaggcgc tggggttcat ctatccctcg cccggctacg tggacgagcg cattgacctg
     5281 ttcttcgcgg aggtggaggc cgagcccggc gcgacgcggc aggacgagga cgaaaacgtc
     5341 gcttccgtcc ggctgactcg agccgaggcg ctggccgaat tgaaggggcg ggtcgtggat
     5401 tccaagacgc tggccgcctg gttgcggagc gagctggccg gtttgtgcgg cggcggttga
     5461 gcggggggag gcggacatgg ccatgggcgt aaaagaaggc tggcagcggc tcaagaactt
     5521 cctgctgcac ggcatatggg aggcggactt gagcgccctg ccgccgggcc gcagcctgcc
     5581 catccgcatc gtccgcatcg gccagctcgt ggtcaaggga ttcgccgagg acgacctcgc
     5641 catccacgcg tccggcctga ccttcgtgac cctgatgtcg atggtgccga tgctggcggt
     5701 gatcttcgcg ctgctgaagg gcttcgggtt cggccaggac cgcatcaacc agatgatgaa
     5761 ctgggtcagc ggcatgccgc ccgaattcca ggccttcgtg gacaacatgc gggacatcgc
     5821 caacgccacc aacttcgccg ccatgggctg ggtcggcctg gtggtcgtgc tgttcacgtc
     5881 ggtgctcgtg ctcggcacga tggagctctc gttcaaccgt atctggggcg tccgggtgaa
     5941 ccgcggggtg ctgcgccagc tggccaacta catcagcatc ctggtgctga tccccctgct
     6001 ggtcgcgggc gtcggcacgg cgcacgcaag cctgcgcggc gggctgctgc tgctcccgaa
     6061 gccgttcagc ctgctggccg gctcggtgct gcgcctgacg accgtggtca cgacgtggct
     6121 cgtcttctgg ttcatctacg tgcacctgcc caacaccaag gtcaagaccc tcccggcgct
     6181 catcagcagc ctcctcggcg cggtgtgctg gctggtctgg cagaaggcct acatctccct
     6241 ccaggtcggc gtggcgcgct acaacgcgat ctacgggacc ttcgcgtcgg tgccgatctt
     6301 cctggcctgg ctctacacca gctgggtgct catcctcctc ggcgccgagc tcgccttcgc
     6361 cctccagaat tccgcgacct accagatcga gcgcgcggcc gaaaacgcca gctccaacgc
     6421 ccggctgatc atcgggctct cggtcctggt ccgggccgcc caggcgctcg ccgggcaggc
     6481 cgcccggttc gagaccgccg cgttcggccg ggagcagcgc gtgcccgtcc gcctgctgaa
     6541 cgacatcgtc cgcctgctcg tgcgcaccgg cttcctggcc gagacgacgg acaaggccgg
     6601 cagctatgtc ctgctccggt cggccgactc gatccgggtc aaggaggtcg tggacgtcgt
     6661 gctgcaggac ggggcgcgcc cggaggccct cggcctcgcc caccgcgatc ccgccatcga
     6721 aagaatcatc gcccagctcg agaccggcgt cgaccggggc ctggaaaaca tgacgctccg
     6781 ggagtgcctc caccccgcga tgtaaacgcc tccgcatcgc ccttccgcct ttcccccatg
     6841 ccccccattg acttcgagaa ggacctgaac cccgagcagc gcgccgcggc gctggccccg
     6901 gacgggccgg tgctcatcat cgcggccgcc ggcacgggca aaacccgcgc gctgacctac
     6961 cgcgtcgccc acctcgtcga gcgcggcgtg gacccgcgtc gcgtcctgct gctgaccttc
     7021 accaaccggg cggcccgcga gatgctcgac cgcgcccgcc tgctcgtcgg cgagggcgtt
     7081 ggcgggctgt ggggcggcac gttccaccac atggccaacc gcatgctgcg gcggcacgcc
     7141 ggggcgctcg gctaccagct cgactacacc atcctcgacc aggacgacgc ccgcgggctg
     7201 gtccgcgagg ccgccgacga gcttggcttg ctgggcaagc acttccccaa gcccgacgtg
     7261 ctgttgagcg tcttcagcct cgccgccaac aaggagcagg cggtggcgga cgtcgccgga
     7321 acctggttca agaaccaccc gatcgacatc aacgacgtcg tccgcgtcca cgagtcctac
     7381 gtgaagcgga agcgcggtct gcaggccatg gacttcgacg atctcctcgt caacggcttg
     7441 cgcctgttcc gcgaggagcc gaacgtgctg cggcagtacc aggagcattt cctgcacatc
     7501 ctcgtggacg agtaccagga caccaacctc atccaggccg aatgggtgga ccgcctcgcc
     7561 gagcagcggc gcaacctgct cgtggttggc gacgacttcc agagcatcta ctcctggcgc
     7621 ggcgcggact tccgcaactt cctgcgcttc ccaaagcgct acccggaggc gacggtcttc
     7681 cggctggaaa ccaactaccg cagcgtgccg ggcgtcctcg aggtggccaa cgcctgtatc
     7741 gccggcaacc cggagcagtt ccagaagacc ctgcgcgccg tgcgcacgga ggagacgcgg
     7801 cccgtgctcg tccgcctgta cggcggcgag gagcaggcgc gctacgtcgt cgaggcgctc
     7861 aagcgcctgc gccgcggcgg agtgccgatg aaccgcgtcg ccatcctcta ccgcgcccac
     7921 ttccacgcga tggagctcca gatggagctc gcccgggaga agatccctta tgtcatcacc
     7981 tccggcgtga ggtttttcga gcaggcccac gtcaaggacg cgtgcagcct gctgcgcctg
     8041 ctggtcaacc ccggcgacga actggccttc cagcggctca tggggatgct gcccaaggtg
     8101 ggccagaaga cggccatccg gatctgggaa ttcctcggca agcgctgcga cttgcgcgac
     8161 cccgcccagg ccgcccggct gcgcgacaag ctcccgcccg ccgcgcggga tagttggaag
     8221 cagatcgaac ccctggccgc ggcctacgtc gaggagcatc tcgacgaaga cccgggcgag
     8281 gtcctgcacc agttcatcga ggccttctac gacgactacg ccgtcgagac tttcgagaac
     8341 gcggagcggc gcctggagga cctcgaagcg ctgatcgact acaccaccaa gttcgagagc
     8401 gccgaggcgt tcttgagcga gacggcgctg atgaccaacc tcgacgccga ggcggacaac
     8461 gtccagggcg agccggaaaa cgcgctcaag ctaagcacga tccaccaggc caagggcctg
     8521 gagtgggacg tcgtgttcat cctgtggctg gccgagaaca tgttcccctc cgcgcgcgcg
     8581 atggaggatg ccggcggcct gtccgaggag cgccgcctct tctacgtcgc cacgacccgc
     8641 gcccgcgacc agctgttcct ctgcgtcccc gagatgcgcc gcacccgcga cggcggcgcg
     8701 atgtactacg ccccgtcccg cttcgtccgc gaactccctc cggggctcgt gaaggaagaa
     8761 aacgtggggt ttatttagga ttcagggttc aggaaaagca gaccgactac cttccagccc
     8821 ccttggacgt tctgctccct tccgcgctac ggccccggcg gaggggtgaa cggcgcggcc
     8881 acgcgataga acttcaaccg gtccggcgac gcgctgaagt ccgtctgcgc gttggccggc
     8941 cccatgatat acccgccgaa cgtctgccag gccgcgttgg tgtcgttcag ccnnnnnnnn
     9001 nnggtccaca ggatccgcgc cgtcacgccg ctccggatga ggttggtgac gacgatgttc
     9061 gtggtgggcc cgggctggta gatggtgatc cagtaatcct ccacctcgcc gtcggaggcc
     9121 agcccggtgg acgacaatcc gcccatgctg ctgaaccgca ggcgggcgcc catctggcct
     9181 acgccgattg ctgcgggcac ggcgaccgtc acgatgttca cgccctgggc cagcgcgtag
     9241 ctcgcacaga tctggtcgcc ggcatcggcc cagtccctgt cccaattgaa gtccatccac
     9301 gcgtccagat aaccgttggt ggaagccgtg accaggatgc ggttggtgcg ccccatgacg
     9361 aaagcgcccg agggcaccat gccgtcctcg tcgtccaggt tgctcaaatc atcgccatcg
     9421 gcgcccccgg agggcatgcc gtcggggtcc atgtccggcg aaatcgcgcc cagccagtac
     9481 gcggaaggaa cccggtgccg cgcgccgtca ttcgccagga gggtcgggta cggcgcccgg
     9541 gtgtcgccga aatcgagggc ctcgaggcgg acctcgtagt cctccacctc gccgttggtt
     9601 tccgccccgg tgaaggccgg cgcgaccggc gcgtgggtgg tgaaccgcca gcgggagtgc
     9661 gggccgcccg gcaccagggc cggcggcagc ggcacgttca cgggcagcag gttgatcccg
     9721 gcgctcaacg gctggttgac gaacacctgc tcgcccgggt tcagccagct catgttgctg
     9781 ttccagtcga tccacgcgtt gaggtagccg ggcacggagg ccaccacggt caccgtggcc
     9841 gtcgcgccgg caacgaacac cgccggcaac acgaccccgt cctcgtcatc cagccccgcc
     9901 ggaggattca ggtcgtcgcc gtccgcgttg atggtcggct ggccgtccaa ttcggtgtct
     9961 atgcgcgcac ccagataaac gccggggatg aaggcatgtc gtgcgccgtt ctggatgaaa
    10021 cgggtcggat agcccacgcc gacgccaccg ccgtcccatg catcaccata atcgaggcct
    10081 tcctcgagcg gctcctgcag cgtgacctga taatcctcca cctcgccgtc gttgaccagg
    10141 ccgtaggggt tcatggacac cgcgagagtg gtgaagcgga aacgggcata ggtggggccg
    10201 gggctggcgc tggccgggac gttgatggtg gcataattca atccctgcgt cacggcgacg
    10261 ccgccgagca cattttcgcc gaaatccccc caggatccgt tggtgttgaa atccacccaa
    10321 acggcgagag tgcccgcgac ggaacattgg accacaacat ccgtggattg gccgataatg
    10381 aaagacccga ggaaggtcac gccgtcctcg tcgtcgaggt tgctattgtc gtctccggtc
    10441 gcggtcgcgt tcggctggcc gtcgagttcg gcgtcgatca gcaggcccat ctgaatgccc
    10501 ggcacgatga catgccgcgc gccgttggtg gccagggaag tcgggtagct cgggtccggc
    10561 gcgtcgccga aatcgagctg ttcgtcgggg gtttcctcgg cgttgtggaa cacggacacg
    10621 tcgctgctgc gcagcggcgg ctccggattt tgcccgacat ccagcgcgat ctcgtcgatc
    10681 cacgtgctga ctttcagcga cgtcgtgcgg ttggccagca catccaactg attggaaaag
    10741 gactgttgaa gcacgggata gcgcgtgtcg aggggggata tcccaggaag ctgcacgctg
    10801 aaatccatct gctgcgtgga gaactgtccc tgcggaatat ccccaaaatc ccacgtcggc
    10861 acgtagaagc cgcccgccgg gtttcgctcc tggttggtgt aatccgggtt ccagcaatac
    10921 caggccgcct gatcctcgtt gtcggcgatg atgtacggcg ggcggtcgcg gtacggggaa
    10981 ttccagatga gcgggaggtt gccgaggtct gtgacggcga actcggccgt cagcttggtg
    11041 tcgtacatgg ggcccacgta ggagagtatg ccagggagga tcagggactc cccgtggctg
    11101 ttgtttccgc tccacgggaa ctccgtctgg ctggtatcgt tgttgatcgt cgcgacgatg
    11161 ctgaaggcga tgatgttgaa cgctgggtcg tacgtgatgc tgctgacata gcccgcgatg
    11221 gcggccgagc cgctgccgcc gccgagcagt tcgtcgtaga actcgtgcgt gcggacggtc
    11281 tcgatgcccg agctgctgct ccggaaccac tgcccggtaa cggcgggggc gtcgcgcggc
    11341 gggccggtca ggatcgtggc gtccgccacc ggcggcttgg tcaccggcag ggagggaccg
    11401 gccagggccg cagccggccc caagaccagg atcagcaccg aaagcccaag agaaagcatc
    11461 aggtatttca tggttcacct tcttgttgcg tctctccatg aaaaggataa ccgtgtcgca
    11521 aagaagctgt caatcagatt ggccttgaga acgggccggc gttgtggtta cgtaccgacc
    11581 cgtcatcggc gaagcggcgt gcgtccatgg cgaactacat tttcattacc ggcggcgtgg
    11641 tttcctccct tggcaaaggg ttgacggcag cctctttagg cagactgctg atcagccgcg
    11701 gcctctccgt ccgcatgctc aaggtggacc cctatctcaa cgtcgacccc ggcacgatga
    11761 gtccctacca gcacggcgag gtgttcgtca ccgacgacgg ggccgagacg gacctggatc
    11821 tcggccacta cgaacggttt acggggcagc cgacctcgaa gaagagcaac ttcaccgccg
    11881 gccgcgtgta ctgggaagtg ctcaagaagg agcggcgcgg cgactacctc ggcgggacgg
    11941 tccagatgat cccgcacatc accgacgaga tcaagagccg catccgcgcc ctcgacgagg
    12001 ccggcgtgga cgtggtgctc tgcgagatcg gcggcacggt cggcgatatc gagggcctga
    12061 ccttcctcga ggccatccgc cagatcggcc tggaggaagg ccgcgagcgg acgatgtaca
    12121 tccacctgac ctacgtcccg ttcatccgcg ccgccggcga ggtgaagacg aagccgtcgc
    12181 agcagagcgt cgcgcggctg cgcgagatcg gcatcgtgcc cgacgtcctg atctgccggt
    12241 gcgaggtccc gctttccgag gacgtccgca agaagctgtc gctgttctgc aacgtgccgc
    12301 tccacgcggt gatcgaggag caggacgtcc gccacacgat ctacgagctg cccctcgtcc
    12361 tcgccgagca gggcctggac gagctggtcc tggtccggtt cggcatggcg cgcccgcccg
    12421 cccgcctggc gccctggcgc aagctggtgg acggcatcgt ccacccggag ggcgcagtgc
    12481 ggatcgccgt ggtcggcaag tactccgagc tgcaggacgc ctacaagtcg ctgttcgagg
    12541 ccatccacca cgggggcttt gcccaccggc ggcgcgcgga gatcctgaag atcgccgccg
    12601 aggaggtcga ggacgggcag caccggaaga cgctgcgcgg cgcggacggg atcctcgtgc
    12661 cgggcggctt cggcacgcgc gggatcgagg gcaagatcga ggcgatccgg atcgcccgcg
    12721 agcggaaaat cccgttcctg ggcatctgcc tcggcatgca gtgcgcggtc atcgagttcg
    12781 cccggaacgt cggcggcctg aagggcgccc acaccaccga gatcgacaag caggcccccc
    12841 accccgtgat ttgcctgatg gaagagcagg aggccgtcgc ggagttgggc ggcacgatgc
    12901 ggctgggggc ctggccctgc gcgctgaaaa aggggtcgca ggcggcgaag gcgtacggcg
    12961 gcgccgcggt cagcgagcgc catcgccacc gctacgagtt caacaacaag taccgcgcgg
    13021 aactggagaa aaagggcctg atgctgtccg gcctgtcgcc ggacgggcgc ctcgtcgaag
    13081 ccgtcgaact gaagaaccac ccctggttcg tcgccgtcca gttccacccg gaattcaagt
    13141 cgcagccgct cgcgccgcac ccgctcttca aggcgttcat cggcgcggct tgcaagaaga
    13201 agagcggatg aaccgcggag gacacagagg tcacggagga accaatacat gcgttagcaa
    13261 ttccgccccc cctctccgcg ccctctgcga cctctgtggt gaaaatcgga taacgaaatc
    13321 atgaaagcct tgatcgcatt ggaagacggg acctgcttcg agggccgcgc gtttgccggg
    13381 ccgggcgagg cgcacgggga actggtcttc aacacctcga tgaccggcta ccaggagatc
    13441 ctgaccgatc cgtcgtacca cggccagatc gtcacgctca cctatccgct gatcggcaac
    13501 tacggcatca acgcggagga cgtcgagtcc ccgcgcgcgc aggccgccgg cctggtcgtc
    13561 ggcgagtgca gccgcatccc gagcaactgg cgcgcgacga aaacgctgcc cgactacctg
    13621 gccgagcatg gcatcctggg catcgaccac gtggacacgc gggccgtcac gctccacatc
    13681 cgctcgcgcg gcgcgatgaa gtgcgtgatg tccaccacgg acctggaccc ggcccgcctc
    13741 gtgcagaagg ccaaggactc gcccggactg gtcggccgcg acatctgcac cgaggtcagc
    13801 acgccggccc cctatgcctg gcccgggccg catgacgggc tgaaggcctt ctccaccatc
    13861 cgggaggacg ggctgaaggc ctacgacagc gtcttcggcg cgccgcccgc ggcgccacgc
    13921 ttccgcgtgg cggtgatgga ctgcggcatc aagctcaacc agctccggct gctcgcccgg
    13981 ctcggctgcc ggtgcgaggt gttctccaac tccacatccg cggcggcgat cctggcctcg
    14041 aagccggacg ggttcttcat ctccaacggc ccgggcgacc cggagggcgt gccgcacacg
    14101 gtcgccgaga tccggaagat cgtcgagacc ggcctgccga cgttcggcat ctgcttcggg
    14161 caccagctgc tcgggcttgc gttcggcggc cggacctata agctcaagtt cggccaccgg
    14221 ggcgggaacc agccggtcaa gaaccggctc accggccgcg tggagattac gtcgcagaac
    14281 cacgggttct gcgtggacaa ggattcgctg gacccggccg ccgtcgagac gacgcacgtc
    14341 aacctgaacg acggcacatc cgagggcctg cggcaccgga agctcccggt catggccgtg
    14401 cagtaccacc ccgaggcctg cccgggcccg cacgactcga cctacctctt ccggcagttc
    14461 atcgggctga tggattccgc gaatcgttag ggcgcggcac aacgtggaac ggccgggacg
    14521 gccgtgccgc gcgagcctcc gaacagaatc cgcgggctgt ccgttcggcc gcggctcacg
    14581 gcgcgcgctg tgtgaagtcc ggcatcgtat cggttacgat ctcgaaggcc atggccttgt
    14641 cttcgcgctg cacttccagg gtgatggttt cgccggcgcg gcgcttgaag atctcccgct
    14701 gcacggcccg gggcgtggcc acggcgacgc cgttgacctt gataatcagg tcgccctccg
    14761 ccatgccgac gcgcgaggcc ggcgtgttgt taaagacttc cagcacccgc accccggcct
    14821 cgcggcggac cgaccggcgg gccagcgggg agtcgtccac ttcacccatc tggataccga
    14881 tccacggccg ctcgacttct ccccgctcga tcagctgctc ggccacgcgc atggcgaggt
    14941 tgaccgggac ggcaaaaccg atcccgatgc tcccctggac catcgggccg ccggtctgga
    15001 tcatcgcgtt gatgccgacc atccgcccgt ccacgtccac cagcgggccg ccgctgttgc
    15061 cggggttgat cgaggcgtcg gtctgtatga aatcctcgta cggcagccgc ccgacggagc
    15121 ggcccttctg gctgacgatg ccgagggtga cgctgctgtc gaggctgaac ggcgaaccga
    15181 cggcgatcac gaactcgccg acgcgcaggg cgtccgagtc gccgggctcg atggcggtga
    15241 acttcgtctc cgccggcgcc ttgatcttca gcacggccag gtcggtcagc gggtcgcggc
    15301 cgacgaggtc cgccgggtac ttcgtgccgt cgtcgaggac cacctcgacc tgttccgcgc
    15361 ccgcgatgac gtggtggctg gtcagcacgt aaccgtccgg gcgcacgatc acgccggagc
    15421 cctgcccggc caggcgctcg ggaatgccgt agagggtgcc gaagaacatg tcgcgcgccg
    15481 ggtggtagac ggtcgcctcc gtgcggacga ccacgacggc gggcatcacc cgctccacgg
    15541 cgtcggccac ctccgcgccg aactggcggg ccgccgaagg ccccgccgcc agcgccgtcc
    15601 acgcccagaa ccaaccgcac accgccgcca gccctatcct tttcatgtct tgcttccttt
    15661 ctgcaggtag acaacgcggc gaagacttcc gttcagcgcc gccgtccgcc cggcaaaacc
    15721 ccttgcccgc cgggtggaag cttggtaagc gttacggcaa tccgggcccg gaacgggcgg
    15781 cggcccggcg agcgaccatg agcctgatca ctccagtctc cgcgcaacca ggcacagccg
    15841 ggcggcccct gtccccctcc gctccagccg tcagcgtggt ggtgccgttc aacgcctgga
    15901 acgactgggt gatggagtgc ctgcaaggcc tgcgccggca gaccgtcccc gacttcgacg
    15961 tctggctcgc gcccgacgcc ctcccgccgg aaccgtaccg cgcccgcctc gacgccatgg
    16021 gctggggccc gcggctgcac gtcgagccca cgggtcccgg caacccctcg cggaagcgca
    16081 acgcggccct cgcccggtcc gcctcgccgg tggcggccct tgtcgattcc gacgcctggc
    16141 cgcgcgagga ctggctggag aaagccgtcg ctttgctcga cgacgaaacg gccgtcgtga
    16201 ccggccccaa cctcacgccg cccggcgatg aatggccgcg ccgcgtggcg ggtcgggtca
    16261 tggaaagccc tctggggttc ggcggcggat acatccggca cgtgcccgcc ccgcgccagg
    16321 tcgtccggga gatgccgacc tgcaacatgg tcctgcgccg gctggagggg ctgcttttcc
    16381 gcgaggacct ggataccggc gaggacatga tgtattgctc cgatgtccgg gcgcgcggga
    16441 agcgggtcct gtacgatccc gaggtcgtgg tgtttcacca tcggcgccgc ctcttccgcc
    16501 cgttcatgcg gcagttctac gcctacggct tccacaaagg ccgcctggcg cgggccggct
    16561 cggacatcac gtacctgtgg caggccttcc cggccctgct ggtgctgtac ctggcgggct
    16621 gggcggtcag cctgctgctc ccgctgcccc cggcgctgcg ctggctcgcg gcggcgccgg
    16681 gcctgctcta cgccgtggcg atcctggccg agtccctgcg acgggggcgc tgcgccgccg
    16741 agcggatggc ggcgcccgcg gccttcgcgg cggcgcacgc ggcctacggg accggctacc
    16801 tggcgggctt gctctcccgc cgatgagccc ggccgggatt tctcgttgac aacgggccgc
    16861 gccggccgac actctcttcc gatcaccatg aagatcagcg gcttcacgtt catccgcaac
    16921 ggccacatgc tgggcttccc gttcgaggag tccatccggt ccgtgctgcc gctggtggac
    16981 gagttcatcg tggccatggg cgcgtcgcag gacgacacgg aggagaggct gcgcgccatc
    17041 cgcgacccga agctgcggat catcccgacg acgtggaacg agcgcatccg gagcgacctg
    17101 ggcgtgaagg gcttcgtgta cggccagcag aagtccatcg cgctgttcaa ctgcacgggc
    17161 gactgggcgt tctacctgga gggcgacgag ctcatccacg aggacgacat cccgaagatc
    17221 cgcgcgtcca tggagcggaa cctggacaac ccgcgggtcg aggcgctgta cttccgctat
    17281 ctgcacttct acggcaatgg ccagacgctg gcctcctcgc cggcctggta ccgccgcgag
    17341 gtccggatcc tgcgcaacac gatcccggcg tgggcgccca agggcctgtt cttcatcacg
    17401 ctgacgtccc ccaagggggg gcgctacccg cgcgcggccg acagcgacgg cgcgatctac
    17461 cactacggct gggtgcgctc cgaacagcag atgattgaga agttcgacca ggtcggcaag
    17521 ttctggccgg gccagaagcc ggagatcaag ctcgacgaga tcgatccgca gtccttgaag
    17581 ccatacacgg gcacgcaccc ggcggccatc cgcgactggc tgccgcccgc gacgggcctg
    17641 ttccaggcca acccgaacca cgtcctgacc cgccgcgagc gcaagaaccg ctgggtcgcg
    17701 cgcctcgagc gcctgacccg cgcggatttc tcgaagcggc acttcacctt cgcctgacca
    17761 accctctgaa cccggtggaa ttcccggggt atgttttcac ccacccggac aatgccatgg
    17821 ccaactcacg ttgcgtcggg ctgatcgccg tcctcgtcgg cgggatggaa gtgaatgcgt
    17881 ccgcccgcat gtggccggat tcctcgagtc ggatcgtcgt cttttccgat cagctccagt
    17941 ccggcctgac cgccgcccag cgccagttcg ccgcaacgaa cctcgccggc acccagaaaa
    18001 tgcggcgggc ggacatccag gcgctccgcg cctacaacac gaatttcctc tgcctccatt
    18061 accagctcgg cgtcggcaac gggcccgagc tgttcctcga cggcgacgca tggacctcgg
    18121 actggagctt cgtcaccacg caaaccaact ggttcatgct caacgcgcag acccagcgcg
    18181 tctaccacac gacgtggaac tggtacctga tggattgcac gtacacgagc ggcctgccgc
    18241 gcaacgggtt ccccaactac tggatcacca cctgcctcgc ccgcatccga tccacggaag
    18301 gcgacggggt ctttgcggac agcttcacgc aggacgccta cagcttcggc cagtgcagcc
    18361 cgacccatcc ctggatcgag gacctcgggt tgtgcgtcag ccactggatc ccctcgctgc
    18421 actccttcgg cgcggcctgc cgggcggcct tcgaggccga tcccggcggt tttctctacc
    18481 tgccgaacct cggcggcctg atcacgtcgt gggacccgac ggacttcggg ctcggccacg
    18541 gtggaatgat cgagggcttc tgttactggg gcaccggcaa ctacttcgac cgggacgact
    18601 ggcggctgca aatggatcgc gcgctggcgc tggtccggac cggcaagacc gtgatctgcc
    18661 agagctaccc cgacacctcc gcgtactccg accggatgtt cgcgatcgcg agctacctgc
    18721 tggtcaaggg cgcgcggacg tatatctgcc tgctgaccac gggcgacgtg gcgttggaat
    18781 actatcccga gtacacgatc gcgctcggcg gctcgacctc gcccccgccg gcggccacca
    18841 cctcggcctg gtacggggcc tggggcgtct accgccgcgg gtatacgaac gggtggacgc
    18901 tggtgaaccc cggtggcagc gcggtgaaca tttccaatct tgggggcacg ttctaccgcg
    18961 tcaccgccag cggcggcggc gcggtggacg gcgcggggaa ttacggcggg agcctctcca
    19021 ccgcgccggt cacgtcgctg accgtggcgg cgtactcggg cgaggtcctc ctgttccagt
    19081 cgcaggcgcc cagcaacacc cagccggcgg cgctgaccac cgtccaccgc tcgggtcaga
    19141 cgttcatcac gtggcgcgag cgcagcgacc tgtcgggcga gcgctaccgc gtgtaccggc
    19201 acgccgcgcc gatctcgacg accaacctgg cgcaggccgt gccgctctac acgctccaga
    19261 agggctccgc ccgcttccac gccaaccggt acaacgtcgg aggtggcggc acgtgggcgc
    19321 cgcgctacac cgaccggttg gtcatcacca acggcgggcc ggcgctgggc gtgaacacgg
    19381 gcttgctggt ctggacgctg gcgacaaacg atttcggcgg cgcaacgacg ggcgatgcgt
    19441 actacgcggt cacgctgctg gtcggggcga cggagaacac cggcgacttc tcgtcggcga
    19501 acagcatcgg accggtcgcg gagggcgtgg ccgatcccct gcccgtcctg gtcaccaatt
    19561 acccggccgt tcccggaggc ggagcggtgc ggatatacat ccagtacatg gacctgcgga
    19621 actggaaccc gtccttccac gcgccgcacg cgcgcaacgg cttctacggg cagagcgggc
    19681 tcgacccggc cgtcaccggc gcgatccagt acgcctacga ttatgccgtg gtcgagccgt
    19741 cgtgcgtcac cagcccggcg ccggcctaca tcagcctcca cggctggtcc ggcaacacgt
    19801 acgggccggt gacctccgac cccgacgcct acgactggtg ctgctacaag attttcccga
    19861 ccgacatgag cgagacctgg ttcttcgggt tcgcgcggaa ctgcgactac cggacggacg
    19921 ccgatcccgc ggccggcgac acgatcgata acttcaccga gcgccgggtt ctgcgcatgc
    19981 tctacgacct cattcgccag ccgcccggcc cgccggtgga tacgaatcgc gtgtacgtct
    20041 tcggcggatc catgggcgga agcggcgcgc tggcgctcgc gctccgatat ccccacgtct
    20101 tcgccgccgc gtacgccagc cagccgatga ccgattactc cacgcagggc gacgcgggcg
    20161 gcacgccgtg gagggacgac atcgagtgga aatggggaag cgttgttcta aatttgccca
    20221 ttcgcagcga tgcgccggga ggctgggcgg atcacttgaa ggccttggac gggactggga
    20281 tctggacgtg gcagaatcat cggcgcaccg cgtccaacgc cgtcgcgcgg gccacggtgc
    20341 cgctggggat cggccacggc acgaacgatt tcgtcatcga gtggccgacg cagggtgaac
    20401 cggtctaccc ggcgttcaac gcgggccggc gctgctgggg cggcgcggtc attgacgccg
    20461 ggcattcgtg gatggggtgg aacggtctgc cgccgacgat cgccccagat ggctcgctcg
    20521 ctcccttccg gcgcttcacg gtgcgtaagg atgaatccgt acccggccta agcaatgccg
    20581 aaggcgacgc gcccctccct ccagccggac ccggcggata caaccaggaa ctcgagtggt
    20641 cggccggttg gtacgactgg gacagcgcgc cgacggatgc gaccaaccgg tggcaggtca
    20701 gcctccgcag cctgaacggc gcaacgcaga cggtggatgt caccgcccgc cggctgcagc
    20761 gcttcccggt cgcggcagga acccgggtgc tatggcggaa tgtcctggtc gccaacggga
    20821 ccacgtcaca ggtcggcgtg gcggcggcgg acggcgaggg cctgatcacc gtcccgcagg
    20881 tccgggtgac cccggacggc aaccggctgc ggctggacgc cgacaccggg cccgacagcg
    20941 acggcgacgg aatacgggac tactgggagg atgccttcgg cttcaatagc gcctccacga
    21001 acgacgcgag gtcagacgct gacggcgacc ggtcgcccaa cctcgacgag tactgcgccg
    21061 gcacgctgcc ccgcgacgcc tcctcggttt tccgcattac ccggtacgac gacggcagtc
    21121 ttgcctggct cggtgtcccg ggcttccgct acgcgctgga gaccacgccc gcgctggcca
    21181 cggctttcgc aggccacgcc acagtgaccg cggccacatt tcaatccgag gcggttgtcc
    21241 ccttcggcga cagcacgtcc cggttctggc gcgtgcgggt ggactactga cggggccgtc
    21301 cggcgaagcg gggatcgttt tcgtcgaagc ggaagacgac gtcctgcccg gcggcgaggc
    21361 gcaggacggt attgaacccg ccgcgcgcgt cgtcctggaa ctcggctccg cgcgcgtcgc
    21421 agagccagcg ccggtaatgg ccgaccatga acttgtagtg gtgcacgccc gcgggcaggc
    21481 tctgcgtaac ctcccacacg cccttgctgc cgcgcgtcat cggcaggttc tcggtccagt
    21541 cctggagcca gtcgccaagc aggtagacct ggtgcacgtc gtcgggaagc tggatgcgct
    21601 cgaggcggac cgccaggtcg aatccgtcct tcatctcggg cagcaggacc agccgctggc
    21661 gatggcgctc gtagaccggc gtggagatca ccagctcgta ttcgcccgcg ggcagcgggc
    21721 aggtaaagcg gccttccgcg tccacgtccg cgtaccagac cggccgctcg acggggcgcg
    21781 cgtcctccga agcccggccc tccacgcgga gatgaaccgg cgagggccgc cgcccgtccc
    21841 acgtctcgat ggaaccgcgc agggtcaccc gggccgcgtc ccgggccttt tcccatcgcg
    21901 cgagggccgc cggggccttg ctccggagca actccgccac ggcccgggtg tcctcgcccg
    21961 cgaggtccag aagcttcgcg gcggcggcgt ccgcgatccg cgcgtccggg ctgtcgagca
    22021 gggcctccag cagcgcccgg ccatccgcat cgcggccccc gcccagccgg caatacgcca
    22081 gccgcaaacg ggccgattcc cgccaggccg gttccgcccg ctcgtcgttc gccagccgct
    22141 cgtagcgttc cgccgcggcc gcgaagtcgc ccgccccctg ctcgaggtag taggcttcct
    22201 cccaggcgac ggcggggtcg ggcgcgtcct ccgcccgcgc gggcgccggc agcctcgccg
    22261 ccagcatgac cagccaaccg attctccaca tcgttttcat ggttcgaatt tgtatccgaa
    22321 ggtgcgcacg gtcagcaggt gggccggccg gcgcgtgtct ttctcgattt tctgccggag
    22381 cacggtgacg aaccggtcca cggtccggtc cgtgccctcg tagtccaggc cccagacccg
    22441 gtcgaggatc tgctcgcggc tcaatacgcg cccggcgttc agcgtcatgt attccagcag
    22501 gccgtactcc cgcggcgtca gaggaacggg ctcgccgccc cggcgcagcg tcttggcctg
    22561 cacgtcgatc acgcaatccc cgaaggcgac ctgcggcgcc gccggcgcgg cggacgcgcg
    22621 gtgcagcagg gcctgcatcc gggccgcgag cgtgtccacc ttgaacggct tgcggacgaa
    22681 atcgtccgcg ccgacctgga agctcaagag ctcgtcctca tcggccgtgc gcgcggtcag
    22741 gatgaggatg ggcacgttca tgttcagttc gcggagccgc cggcatacgt cgtacccgtt
    22801 caaccggggc agcatgaggt ccagcagcac caggtcgggc ttcgcctcgc gggcaagccg
    22861 cagggcggtc gccccgtcgc cggccccgac gacctcgtat tcgcgcttgc gcagttcgag
    22921 ctcaagcccc gtgcgcaggg ccacgttgtc ctcgacgatc aggacgcgca gtttcacggc
    22981 tccgctcccc cgcccagcgg aaggcggatt tcgaacacgg aaccatggcc cggcgtggag
    23041 cggacgccga cgcgcccgcc gtgcgcccgg acggcggcct gcacgatggc gagccccacg
    23101 ccgatcccgc ctacgctcgc ggagagggtc gtgtcggccc ggtagtacgg cttgaagatg
    23161 ctccgcagga cgtcgggcgg catgccgatg ccgtggtccg tcacggtcag cacggcctgc
    23221 ggtccatcgc ggcgcagcgc catctcgatc cggggcggcg gctcgctgta cttcaccgcg
    23281 ttgtcgagca ggttcaggat cgcctggctg accgcctgta tatcgaagcg tcccgcgtag
    23341 tttccggccg ccacatccac cgggagcgcg tccccggcgc cggactccac ggaaaagagc
    23401 cgcgcggcct gccggacgat gtcgtccagg cgtccctcgc tgaaggccag gcgccgggcg
    23461 gcgcggcggc ccctcgcgta ggccagcaac cgctcgacca gttgctggat ccggccgctt
    23521 tccgtccgca acagtccggc gacctggcgg cgggtgggct cgtcgagcga cgagttctcc
    23581 aaggactccg tcaggacgga gatcgcggcg accggcgtgc gcagttcgtg cccgacgcgg
    23641 tcgagcatgt cggcctgcag tttcgcaagg ttcatggaac gcccgacccg gtagagcacc
    23701 gcgccgagcc cggccagcaa ccaggccacc gagatgccga aggtccacgt caggtagcgt
    23761 gttcgcgagg ccgcgagccg gaggaacagc gacgaggagt ccaggtcgta ccgcacggcc
    23821 acgagatcga gcggggcggg aagcgtttga gacaggacgg aaggccgatc cggcgcgccg
    23881 gccgcggacc agtccagcga gccgcgatgg acagccgtga accgccggaa caccggcccc
    23941 gcgacccgcg ccacgaaccc cgtcgccgag agcgcccagc cgattcgccg cccgtcccgc
    24001 agcgggcgtt ccagatccac cagcagcagc gaatccggcg cctgcgctgc gccggtcgga
    24061 atcgcccgct gaaagggcag cccggtccac gcggtgaatt ggaacggccc cggatggagc
    24121 cggtccaact cgccgaacca ccgcgccaac ccatccaggc gcgccgcgtc ggccggggcc
    24181 cacggctgga gaaaagccgc caggcaggcc ggttcgccgc gccgcgggaa acggtcaggt
    24241 ctcccgaatt cctccatcac gcgcgcgacc caaaccgagg tcctggctgg aaggcccgcc
    24301 gcagccgcgg tttccagggc gcgcagcgcc gcgggcaggc gcgaagcgct tgcctccgat
    24361 gccgcggtcc accactcctc gtacaggccg agcgcgtttt ccgtccggcc gcccgccatc
    24421 tcgcgcgccg ccatttccgc gaggaccgaa gcgcggacgt ctccgggtag cggggacgac
    24481 gccagcacct cgcggtacag cgatatcgcg cgctccgctt ccccggcggg cgcgcggcgc
    24541 gcgtcgagca gccggctgcg ggcgggatga ccggccgaca gctgcggccg gtcatcggcg
    24601 ggcggagcgg cgtacggccg gtagagcacc gggattgccg gatcgaggaa gaagaccgtt
    24661 tcgatgcacg aatccgtccc gctctcctcg gcgggcgcct cccggggccg cgcccgcgcc
    24721 gccgcggcgc tgtcctccag cttttgcagc agccgcgccc gggcctcgag tccgagggcc
    24781 gccagttcgc cctcgcaggt ctcgcggtac tggcgagcgg ccgcggcccg cacgctgcgg
    24841 acgtgctggt gcgccagcag gaccagcgcg gcgcacggca cgacgacgag gaccagcagc
    24901 aggagcagcg gccgccgggc gtcgtggcga agaaccagat cggacacggg tatctccatc
    24961 gtctttcgcg ggttgcaagc ccgaacctgc ggtcatcctg atgggctcat gttaccggat
    25021 tgttaccgaa aaaaaaagcg cgcggtaata atccggtaac gtgcccgcgc gaggcttcta
    25081 tccgaagaga aaggagcctg ccatgaaaaa ggaaaaacga ttgaccgttc tcgggctgac
    25141 cggcgcgatg ctgctcggcg tggcgacgga aacgttcgcc atcgcgctca cgcgcgggcc
    25201 gtacgtgcag atgggccatt tcaccaacca ggcgacaatc gtctggtaca ccgattcctc
    25261 ctccgacagc ggggtggact acgggctgac cggaagctac ggggcgttcg ccggcggcaa
    25321 cacgggaacc cgccacgagg tgacggttgc ggggctgcag ccgggcacga gctatttcta
    25381 ccgcgtgcgc agcgccgggg tgaacctggc cacgaaccgc ttcaagagcg cgaaggctcc
    25441 gggccagccc ttccggattg ccatgtggag cgattcgcac tacgggaaca cggccggcct
    25501 cgcggcccgc atggcgttgt acgagcccga cctgatgctg gccgcgggag acatcacgga
    25561 tgacggggtg ctttcggaaa tagacaacaa cgcgttctcc ggattcggcg ggctgatgaa
    25621 acaaatcccc gcctattgga cgccgggcaa ccacgacacg cgggacgact tcgcggcgtg
    25681 ccgggaggtt ttcaccctgc cgggcgacga gcggaattac tggttcgagt acgccgacgc
    25741 gcagatcgtt tcgctgaacg cggaagggtt gcccggcacg gcctggctgg acggggcgct
    25801 ggcggcgtcc gccaagccct ggaagatcgt cttcttccac gaaccggccc gctccccggc
    25861 ggccggccac ggcgaagagg aatcgatccg cgaccaatac gtcccggtca tggaacggca
    25921 cggagtccag ctcgcgctcg ccggccacaa tcatttctac tggcgcagca tcccgctcaa
    25981 cggcgtgacc cacctgatca ccggccggtc gggcgaccgg acccgtgacc tgggcgacat
    26041 gccctgctac tcgcaggcag gaatgaacgg gacggaggcc aagtccttcg ccatcgcaga
    26101 tttcgacggg cctttcatgc agatccgcgg gatcaaggaa aacggcgacg tgatcgacga
    26161 catggtgctg gaccgcgaat gttccttcgt gctggacggc gcgctggacg cgtcggccgt
    26221 gcaggtcgcc gcccgcgccg gcgggcagac gatctgggcg gccgtggcgg gccgttacct
    26281 ctacctggcc acggaggacg ccggcgaggg ccgggatgca tacgtcttcg tcgccacggc
    26341 ttccggcggg ctggtcgcca gcccgtgggc caaggcgggc tccgtgatgg cgcacgatgc
    26401 cttcgttgcg gacgaaaacg acagccgcta ctccggctgg ttcaccggcg cgggcgcccc
    26461 gttcaacgac ctcctggccg cgcgctcggc cacgccgtgg tgcaacgggg ccgtgctgga
    26521 aggcgttctg gatctccagg aactgtacgg cgaggtcccc gccgtgatcc gcctcgccgc
    26581 ggccgcgtac gccacggccg acggcggagc gctcgcggcc tccgcccaat gccccgaggg
    26641 caacggcaac ggggacatcg aggccggcga atttgtggtg gtgaacaccg cggacctcgt
    26701 ccagcccttc gcgctggacg gcgccgcgga ccacgacggg tatctcgtgg ccgaccataa
    26761 cggcatgaag ctgtgggcgg cggtgcacgg ggagacgctc tacgtggcca cctgggcggc
    26821 gggcaacggg ggcgggccca acgaccactt catcttcgtc agcgacacgc ccggcacgcc
    26881 cggccctgcc ccgtgggcca aggccggcca ggtggccttc agcgaagcag ccaagccgtt
    26941 cctggccgac gagggcgccg acagctgggt cgcctggcac aacggcgggg tctccgcgcg
    27001 gcaggccgcg gcctccgtca attcgggcca gttggagggg acgttgaacc tcgtcgaggt
    27061 cttcggctcg gtgccggaga cgctgtacat cgcggtcgcg ccctacgcca cggcggacgc
    27121 gggcgccctg tacgcgccgt cccaggtgcc ggagggcgac ggcaacggga acatcgacgc
    27181 cggcgaattt gtcgccatcc ccgtggccgc gatccgcgat gccaacctcg acggcacgct
    27241 cgacatcctc gatcccgccc gagggttcgt catgagtgac ggcgcgtcgg gcgggaactc
    27301 ggccccgtat tccgtcaact ggtcctcgca gcccggccgg aggtaccggg tgcagagcac
    27361 ggacgacctg aaacagccct ttgcgaaccg aacgggcgag ctgtccgcgg accagggcca
    27421 gttcacgatg acctactcgg atgccgcgcc cccggcgaac ggccgtcggt tttaccgtgt
    27481 gcagcttttg gatccgtagg cccgagggcc tgaaataacc gcccggtccg cttgaaagcc
    27541 cgccggaggt gggctacgtt tcgccgccat gatccccctg ttcggccgcg gctacgacgt
    27601 gctcgtcaac gtcacgaaca tctgtgacgc ccggtgcgtc atgtgcaaca tctggaagaa
    27661 ccaggaaggc ccgacatcct ggctgcgccc ggagatgatg cggtccgtcg gcccgctgac
    27721 caccgtcagc ttcgccggag gcgagccgtt cctgcacaag gagatcgtcg agatcgtgcg
    27781 cgccgtccac gggcgcaacc cgcgggccaa ggtggtcttc tccagcaacg ggttccggac
    27841 ggagccgatc gtggagaaag cgggcaggat cctggcgttc cacaaaaacg ttcaggtgac
    27901 catttccctc gacggcgtcg gcgaggtgca cgaccgtatc cgcggcatcc cgggggcgtg
    27961 gaacaaggtc aaccgcacgt tcgaccgcct cggggagatc gggttgaagc agcgcaattt
    28021 cgccttcacg atcacgcggg acaaccacac cgcgctgccg gccgtgtacg cgcacgcccg
    28081 gtcgaagggc gcggggctct cgctggcggt cgcgcagagt tcgaaattcc tgaacgtcga
    28141 gatcccgccg ctcgaattcg aggcggtccg cccccacctg gagcccatca tcctcgcgca
    28201 cctcaagagc cggaacccga tggactggat gcgggccttc ttccagttcg gcgtgctgcg
    28261 ctacctcgcc acgcggcaac ggccgatcgg gtgcgacgcc cttgagcacc agttcatgat
    28321 cgaccaggcc ggcgaggtcc tgtgctgcca tccgctgctg ctccgcgcgg gcaaccttgg
    28381 cgaggccgac ctgccgaaga tcctctcctg ccccgccgcg aacgagctga agaaggacct
    28441 gcgcgcgtgc cacgcctgct gggaagtctg cacggcgcgt tcggccatcc gcgccaacct
    28501 gttccgggtc ggcctgtggg cggcggggaa caagattctc gcgcacctcg ggctttggag
    28561 cgggaagaaa ggctcccggc tgttcccgct gacccgggaa cgtccaccgc tcaacagcca
    28621 acgtccgaca ccccgcgaat ctccatgacc acgccctctc gacacaccag ccgcgtcgtt
    28681 tcccttcagc ccctctcgga ttcggggtat ctcctgacgt tcgagcgcgg gggcctcgag
    28741 ttcgaggccg ggcggctgct gacgatccac ggccgcgtcc cgaccgagga ccgcagctac
    28801 acgatcgcca gcggggaaaa ggacgagcat ctccaggtcc tcttccggca catcccgaca
    28861 ggccaactca cgccgcagct cgcgcagaag aacccgggcg acccgatcga gttctccggg
    28921 ccgttcggcg agttcgtcgt gcgcgatccc tcccacccgg tgatcttcat cgccacgggc
    28981 acgggcgttg cgccctgccg ggcctatctc cgcacgcacc cgggcctgcc gctgacgctg
    29041 gtccacggcg tgcgcgtggc gcaggacctg ttcttccgcg gggagttcga gaaggcgcgc
    29101 tacctcccct gcgtctcgcg ggaggatggg acgggcttcc acggccgggt gacggatttc
    29161 ttcaagtcct tcgacttcgc ccgggacgcg cacttccacc tgtgcggcgc gaacgagatg
    29221 atcttcgaca tgcaggtttt cctgctgaac gcgggcgtgg acgagtcgca catcttcacc
    29281 gaggcgtact actaccgcct gcactcgtaa ttctgccggt ccggcgaccg cccttccatg
    29341 ccctgtcgtc gggacgccgg ccggcttatg aggatggaca gcacctcctc ctgctcgggc
    29401 gtcgggaggc cctgcacgaa ctcgatcctc cggggatggt tgatgagcgc tttctccagc
    29461 cggcgcggcc acaggcgcgg gaccggcccg gggccgaaaa gccctttcgg tcgtcgtggc
    29521 cacggcatcc gatgcgttac tcctggaaac cgcgcaggcc tcgttgcagc gcgcggcgag
    29581 ccaggcgccg atctccacgg atggcgaccc cggaacgccg ccgcggactg gccgcctatt
    29641 tttccagtcg cgcgatcttg tggcggatca tgtccgtgtg gcgcgtggcg tcgccggggg
    29701 agaccatggc gatgcttaag cggcgcgggt cgagaacgcg tcgggcgacc tcgaggacat
    29761 cctcggccgt cacggcctcg atgcgatcga ggacctcgga gggctggatg agccggttgt
    29821 agcacagcag gttctcgccc acccacatca tctggttgct ggcgctctcc agcccgatgc
    29881 ggagctggcc ccgggcgaag tccttcgcgc gccgcagttc ccgcccgccc accggcgtcc
    29941 cgcggaaacg cgacgtctcg gccaggatca ggtccagcgc gcgcggcgcg cgccggcggt
    30001 ccacgcccgc ctgaacgcac agcaggccgg tctcggcaaa cagctgcacg ctgctctgga
    30061 tcgagtaggc caggccgtgc ttctcgcgga cgatctggaa cagccgcgag ctcatgttct
    30121 cgccgaggat gatgctcaac agcttgaggg cgtagcgccg cgggtcgcgc cggccgaaca
    30181 ggcggaaggc catggccacg tgcggctgct ccacctcctt ggcatgaacg gccacgtcgc
    30241 gctggcggac ggcccggtcc acccgccggg gcgccggcgg aacgcggcgc tccagtccgc
    30301 ccagggcccg cgcggcgcgc tcgacgcagg ccgcgtgatc gacccggccc gccagcgcca
    30361 ccagcgtgtt ggccgccacg tagtgccgtt gcttgaattc ccggatgtcc cgccgccgga
    30421 tccgccggac gctttcttcc gtgccgacca gcgaacggcc gagcggatgg tccacccagg
    30481 ccagcgagcc caggatttcc tgcacgagct gttgcggctg gtcgcgatac agggcgatct
    30541 cttccaggat cacgccccgc tccttctcca ggtcgtccgg gtcgaggcgc gggcgccggt
    30601 acatgtcgaa caggatgtcg agcgcctccc actggtattc ggccgcgatg cgcgcgtagt
    30661 agcaggtgga ctcttcctgc gtgaacccgt tgaaatagcc gccccggccc tcgatggcct
    30721 gggagatctc ccgggcgctg cgcgacgagg tgcccttgaa cagcatatgc tcgatgaaat
    30781 ggcagacgcc ggcctgccgg ccggtctcgc accggctccc cacccccgcc cagatgccca
    30841 tggccacgct ctcgagcccg ggcatcacgg ccgtcgcgac gcgcaggccg cccggcagcg
    30901 tgctcgactc gaagttcagg cgcacgccgc ctcccgcagg accgccagcg tcgcccgccc
    30961 ctcgtacagg gccttgccca cgatcgcccc ggccaggttc ggccgccgca gcgcccgcag
    31021 cgcgcgcacg tcctccgcgg acgtcacgcc ccccgaggcg atgacgtcgc aggcgacggc
    31081 ggagcacagc gcgtccatcg ccgaagcgtt gaccccggcc agcatgccgt cgcgggacgt
    31141 atccgtgtag atcaggcagc ggacgcccat gccttccatc cggcgggcca ggtccgtcgc
    31201 cgtcatcggc gtggtctccg tccagccctt cacctggacg cggccgtccc gggcgtcgat
    31261 gcccacggcc agccgggcgc cgaaccgggc ggccagcgac ttcacctgct ccggttcggc
    31321 gcacgcgcgc gtgccgagga tcgcccggcg cgcgccggca tcgagcgccg cgtcgatgtc
    31381 cgcatccgtc cgcaggccgc cgccgaattc gaccgggatg gcaatcgccc gcgcaatggc
    31441 gcgcaggacg gacaggtgcg cgggacggcc ctggaaggcg ccgtcgaggt ccacgacgtg
    31501 gagccattcc gcgccctcgt cccgccagcg ccgggccatc tcgacgggat cgtcggaata
    31561 gaccacggcg tcgtccgcgc gcccctgccg gagccgcacg cagcgcccgc ctttcaggtc
    31621 gatggccggg aggatgatca tcggcggtcc gcccaccgcc cgaaattgcg gagcatttgc
    31681 agcccgtttt tctggctctt ctccgggtga aactggacgg cgaggacgtt gtcgcgccac
    31741 acggccgagg tgtactccag cccgtactcc gtcgcgccgg cgtcgacagc cgtgttcgtt
    31801 ttttcggcgt agtaggagtg cacgaaatag aaatacgcgc cgtccgggac ctccgcgaac
    31861 agcgggcaat cccggcggac ctggcgcacc cggttccagc cgatctgcgg cactttccac
    31921 tccggcggca ggttgaaccg gcggacgcgg cccgggagga cgcccaatcc cggcacgccg
    31981 ggggattcct cgctgccctc gtacagcacc tgcaagccga ggcagatgcc caggaacggc
    32041 cggctggccc cgatccactc gcgcaccgct tccacgtagc cgttccggac gagatgatcc
    32101 atgcagtcgc cgaacgcgcc gacgcccggc aggatcacgg cgcggcaggc cttcatctcc
    32161 tccggccggg ccaggataga gaacggcagc cccaggaagc ggcaggcgtt gctgacgctt
    32221 cccaggttgc ccatgccgta atcgatgatg ccgatcatga gatgagttac ccgcccccca
    32281 tccagcgccc caggccggcg gcccgccgcg ggctagatac ggcccttgct cgacggcacg
    32341 ccccggaccc gcggatcgcg ggcgcaggcc atgcccagcg cgcgcgccac ggccttgaac
    32401 acggattcgt agatgtggtg cggctccttc ccgtacatcg tcttgatgtg caggttcatc
    32461 cgcccctgga ccgtgaaggc gcggaaaaac tcctcgatga ggccgagatc gaaatcccgg
    32521 atcttccgtt ttcgcggctc cacctcgtag accaggtacg gccggccgcc gagatccatg
    32581 gccacgcggc tcaaggattc gtccatcggc accacggccc agccgtagcg cacgatgccc
    32641 ttgcggtcgc cgagggcgcg gttcagcgcc tcgccgagca cgagcccgac gtcctcgacg
    32701 gtgtgatgat aatccacggc caggtcgccc ttggcgcgca gttccaggtt gcaaaggctg
    32761 tgccgggcga acagctccag catgtggttg aggaagggaa tgccggtgcg gacgcgcgcc
    32821 tcgccggcgc cgtcgagggc gagtttcgcc cggatcgacg tttcgcgcgt ctcccgcttg
    32881 atctcggccg ctcgtttctt catgttcgcc tttcgccggg gacggtcccg gactgcggca
    32941 ccattatagg ccgcgggggg ccggcgtcaa accggggtaa ggagggcccg ccgcactagg
    33001 cctccgagct cgttcgccgg acgcagagga tggcgatgtc gtccgactgc ggcatgtcct
    33061 gcacgaaatc cttcacgccg tgcatgatgg tctgcacgat ccgggccgct gcgtcgcacg
    33121 cgcggcatcg ggagagcaac gcttccaggc gcgcgtagcc gtacaactgc acgttccggt
    33181 ccgtggcctc cgtgacgccg tcggtgaaca cgacgaggcc gtcgcccacc gccaaccgca
    33241 cccggcgggc ctggtaatcg gcctcctcga tgaccccgag aggcgggcca ggcggcaggt
    33301 ccagcaaccg gcagccgccg atcacgggca tcacgaacgg cggtaggtgg ccggcgttcg
    33361 tgtacaccag ctccccggtg cgtgtgtcca ggatgcccat ccaggccgtc aggaacatgc
    33421 acaggtcgtt gccctgcagc aggtcgcggt tcaccatctt gaagatggca tcgggctcga
    33481 cggactcctt gatggcgata tgccgcagca gcgcgatgct ccgggacatg aacagggacg
    33541 ccgggacgcc cttgtccgac acgtcgccga ccaccagggc caggcgatcg tcgtccacga
    33601 agaaataatc gtacaggtcg ccgccgacgt cccgggcggg caccagctcc gccaccaggt
    33661 cgatttcccg ccgggcctcg atctccgccg cgtgcctcgg cagcatgttc atctggatct
    33721 cgtgggcgat ccgcagttcg ctctgcatgc gctcccgcgc ggcgatcgtc tccttcaggt
    33781 tcttcaggta ctccttcagc cgctgctcca tgctgctcat ggtggcggcc aggcggctga
    33841 cctcgtccgg gaaggtgatg gcgaggatgt ccttcgcctc gtcctccggc atcgcgaaat
    33901 cgctggcctg gagccggtcg gcatggtgcg tgagccgggc cagcgggcgc gtcagccggg
    33961 agaccagaaa gtaggcgccg agcatgccga aaagcacgat aacgcccacg taccgggcct
    34021 ggcgcttgat gatcctccgg gccggtttct ccatctcgtc cttgtatacc gacgaggcca
    34081 ggtaccagtc gagcggctcg aatttttcga cgtgcgcgta cttggtgtac acgtggcgcc
    34141 ccagatcgcc cggcttgtcc cactggtact ggtacggcac cccgggattt tccgccgccc
    34201 gcacgaaatt ggtgaagagc gatcccgcca tcgccgtgtc gatggaggtc atcgacgggt
    34261 ggatcaggaa ccgcccgtat tcgtcgaaga ggaaaaagta gcccgtctcc gccaccttca
    34321 cctggctgaa cgtcgttttc agcacctcca tgatctcgcc cattcgccgg gcggcgtccg
    34381 cgtcgatgtc gtcgatgtac accccggtcc cgacgtacca gccccagttt gtgtagggtt
    34441 ggaaatacag cagtttcggc accgggttgg tctcgccgcg ccgcggccaa gctatcgtgc
    34501 agctccattc accggtttga aagatctccg actccatcgt cgccaggatg gggcggcccc
    34561 gcgagtcgag ggtcgccgac atgttgcgcc cccgcacatt cgggtccggg tgcgaaacag
    34621 cgcagtggtt ggtgtcgtag atgatgaagt aatcgtcgtg gccgtaccgg aaatcctgca
    34681 cggtttccag ggcccgccgc cgcgcctccg gctcggtcat ggcgccctgc tcgtacagct
    34741 tgtgatacgt gtcgatgtgc gagacgacca cgccggtcag gttccgcaac tgctgctggt
    34801 gccggtccgt cgcgtactgg cggaacgcct cgaggtcctt gtactggctc gcgatgttca
    34861 ggttgaccac gcggaggacg ttcagcgcgg attcgtcgcc cgaggcgagc acggcccggg
    34921 tcacttccgc gcgctcgaac agcatgaagg cgacggccgt cgccagcagc atgccgagga
    34981 ccatcagcac gatgagcaac ttgaaattgc tgaagaattt catgcggggg caagaggccc
    35041 gagcgcttcc cgcgcggctt ggaataccct tccaatgcgc cgcgattccg tgatccgccc
    35101 ctcgtcgtcg ccggttttca cgttgggggt aaattcatcg aaaccgcccg gcatggcaat
    35161 ctttttcccg ccggccggcg ggccctatcc gttctcgcgc ggcgggccca gaaagggcag
    35221 cggattttcc atgaagtcgc ggtagaaacg atagtgctcc gtctcgcggt acgcgaccgg
    35281 ccggatggcg gggccgtcga agccgtagag ccgcgcgccg gggcacgcca gcaggatggg
    35341 cgaatgcgtc gcgatgatga actgcgcgtg gccgtcccgg ctcatggcct ggagcaggcg
    35401 cagcaggtcc agttgccccc gcggcgtcag ggccgtctcg ggctcgtcca ggaagtacag
    35461 gccgggaagg cggtagcgcg cggagaagta ggccatgagc gattgcccgt gcgactgggc
    35521 gccgaagccc cgcggcccaa aaaactcgtg catggcggaa tcgtggtcgg cgacctgcgc
    35581 gaaattgaag aaggtctcgg acgagaagaa cgagcccggc atcggccccc gggcccagcg
    35641 caactcgacg gcgcgaagga acgcctcctc gtcggcggac aggtccgcga acccgcccca
    35701 gatgaagatc ccggcgttcc ggcacaaggc ccgcagcagc gtcgacttgc ccgagccgtt
    35761 ctcccccacg aagaggacca ccccatggtt caggtccagt tccggcgtat ggagcagggc
    35821 cggcaggcaa aaggggtagc gttcgcgcga ggggaggcgg tccgcgtgca ggcgatagcc
    35881 ggccaggtgc atctcacggc cgcccctgcg gcacatccgc ggggccgggc tcgcggagat
    35941 agtgcatcac cggcacgagg gcctggttgg gatcgcccga gccgtcgtag aacaggcaga
    36001 ccatcggcgc gccggtctcg cgctcgacct gcgggaagat gctcgtcgtg atcgtccccg
    36061 gcatgcagaa catcgggctc gcgttgacca cggcccgggc gccgtcccgc cggacgaaca
    36121 ggacggcgcg cccgagcgtc aggatcgcct ccccctcgaa ctgccacggc aggtacttcg
    36181 caccggcctg gatgacctcg gcgatcgagt gctccttccg gtcatgcagg accggctcgg
    36241 ccacctcgta gtaatgatgc tcccacttct cgaaaaacag gttgcgctcg agccatgtgc
    36301 gcaggttctc caggcgcgcc cgcaggccgc tcgtgctgct ggcgacgagg gtctgcaggt
    36361 agttcgtgta gaggatccac tcggagaccg gggccagcca cgcctcgccg cccagttcct
    36421 cgatccggcg gcatacctgc ccgttgatga acgggtcgca ccgcacgtag atctcgccga
    36481 ccacgccgac cagcggccgc ttctccgggc gccgcgcgac ccgcgcgacc tcgtccacga
    36541 gccccgcgag cagcggggtc acgggcccga cgtccttttg ttcgaaggcc ttctcgaggc
    36601 gccgcaagcc ctgttccagc gtcacgtccg tctcgccggg cctctgctcg tacggccgca
    36661 ccttcatgcc cagcttccgc aggatgtcgc cgacgacggc cgcgtgccac agccgcatcc
    36721 gcgccccgct gtcgaggccc gcgtaggcat tttccgccga cgggctcaag atgcggatcc
    36781 cgccccaccc ctgcttctcg aagatgatcg agtccagctt cgcgtactgc ccgaaccggc
    36841 acggcccgca ggctgtcggc atgaagaaga tcacccgctc cggcggcagc ttccggtcct
    36901 gcatggcctt gaccagcgcg ccgaccgtca gcgggcatgg cacgcactcg ccgccggacg
    36961 tgtgcttgtg cccgatggcc agcgtctccg cgttttcctc cagcacgtac gcctcgtacc
    37021 cgacggaccg gaagaccgcc gccagcaacc gcgcgccgca cgggtccatg ggcggcacga
    37081 acagggtccg cccctcgccg ggtccggtca cgcgttgttc ttcaaggttg ggcatgagag
    37141 gggcttcagg gttcaggaag agaacgattc actattcact tccttggcgg cgggcctcgg
    37201 gatcttgctc cacgaatgga aactctccag ggccgcctcg atgcgcgtga ggtaacccgc
    37261 gtccgcgccg tggccgtcga actggaggca caggtagggc ttgccggcgc ggcccatgat
    37321 ctccttgaag tacgtcagga tgtagctgtc cggcccgcac atgaaattcg tgaagtacac
    37381 gccgaagagc cgcggcgacc gggcgacgca ctcggccgcg gcgaggatct tctgaccgta
    37441 gtgccagtac atattgcggt ggccgggcag gatgtcggcg gtgtcgaagg gcagcatgtc
    37501 ctggtagagg acatggtagc ccatctgggc caccttgcgg ggcaggtcca gggtcagccc
    37561 cgggtcggtg gtgttgtacg gccggccgaa gcacacgagg cccagcgcgt tctccttctc
    37621 gatccgctcc agcgcctcgc ggccgagttc gatgcagtgc cggcggaacg ccgcctgggc
    37681 gtccttcgcc gcccgcaggg cccggcgcac cgcggccgcg tcggccttca gcggttcgcc
    37741 cacggccagc ccgatcgcgg ccacctgctc ctcctccggc cggcccaggt ccaccacggg
    37801 ggccagcagg cgcggcccga ggttcatgcc ctcgatggac cggaggacgc ccgggtgcgc
    37861 ctggacgtag cagcagaaat gcgcgtcggt gaatccctcc ggggtgggct cgcgcagcat
    37921 gtagggcacg aacaggtagt ccacgccggg atcctccgcc agcgcgcggg catgcccgtg
    37981 gctcatgacc acgggggcgc agtactcggc cgtcgtgatc tcgccgccgc ggtgcaggat
    38041 cttttccgag gacggcgggg aatacacgac cgccgccccg agctcgcgca gaaaggtccg
    38101 ccagaaggga tagtagccgt acgtgccgag gccccgcagc agcccgacgc gcggtcccgg
    38161 cgcgggagcg gcgggtccgg cgccgtcgga ccaggccgcc gcccgcctct tccagaactc
    38221 gtaggcggag aggtcccgca cgcgcatgcg cttctcgccg tagtcgcgcc cgcacttgag
    38281 cccccacgcc acgacctcgc ggcccgcgcg gatgatggag aggttgcaga ggttgtggca
    38341 caggtcgcag acctcgcggt ccacggtcac cttctcgtcc gcgaagccca ggcccttgaa
    38401 ccgcgccgag cgctccgcct cggggacgcg ctcgcgggcc agcagggaca tcccgagcga
    38461 gccggtgaga tggcagaacg ggctgacctc gatcggcttc tgcaactccg tctcgaaggc
    38521 cgcgaccagc gccttgttgc gggccgtcgc cccctgaaag tagacgtggt cgccgatgtg
    38581 cagcccgccg acgaccttgt tgaggtagtt gtcgcgcacg gagtgcagca cggcggccgc
    38641 gatctggtcc ttgctccagc cccgcgccat cagcaggtcc aggtcgcgtt ccatgtacac
    38701 cgtgcaccgg tcggaggtcc ggggacaggg ctgg
//
LOCUS       DAOOTK010000021        37872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_797_length_37872_cov_5.694899, whole genome shotgun sequence.
ACCESSION   DAOOTK010000021 DAOOTK010000000
VERSION     DAOOTK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 37872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37872
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_37872_cov_5.694899"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PK634_04240"
     CDS             complement(<1..970)
                     /locus_tag="PK634_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96977.1"
                     /translation="MDGRTQTMAGSEEPTPLLGGVSHRRWTGLAAASLIVLLLFALLY
                     ISARRSVINEIRHQAMGIAIAVAESLDPADLEQVHKPEHIGTPPYQRVQHYLGRVVSS
                     NSDIRYIYTMRRALREDAREDDYEFVVDGPARDIDEDGVIAADEESELPGKAYSARHL
                     PELVAAWRQPMADPEVSPDPPYPDLMSGYAPVHNERGQTVAIVGADITATTVRAKLLA
                     LQIVIATVWVVLTLLMIMVVSLFYREQEALETNRALAAELAGRNEMLRAANTELAHNN
                     EQFVREMKLAQSVQLGFLPKSFPRQDKIVFDRYYLTCDMLGGDLFDV"
     gene            1149..>2440
                     /locus_tag="PK634_04245"
     CDS             1149..>2440
                     /locus_tag="PK634_04245"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOW96978.1"
                     /translation="MSSTVLIGAQWGDEGKGKIIDVLSARADWVVRYQGGSNAGHTVE
                     IGEQRFVLHLIPSGLLHPACRGIIGNGVVVDPVALLKEIDGLRERGIEPAGRLFVSDR
                     AHVVLPFHRLLDARREQNRAGGTLIGTTKRGIGPAYGDKASRVGLRMADLVAPDLADL
                     LRPRLAEQNRILEALGAPAMDTAAVVAEYQAAAARLAPFVADTIPMLHAAVARGERIL
                     FEGAQGTMLDIDFGTYPFVTSSNATAGGACAGSGVPPHRIGQVVGVVKAYTTRVGEGP
                     FPTELKDETGARLREEGREFGATTGRPRRCGWFDAVVARHAVMINGIDWWAVTKLDVL
                     DALETLRICTAYECDGRRLEHVPADVRVLERCRPVYEDFRGWRSSTREATRFEDLPER
                     ARAYLARLEELTGAPVGILSVGPRRESTLWLKAAGEGA"
     assembly_gap    2441..2450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2473..3168
                     /locus_tag="PK634_04250"
     CDS             2473..3168
                     /locus_tag="PK634_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOW96979.1"
                     /translation="MPVHVAIIMDGNGRWAKRRGLPRLKGHQEGAESVRAIIRACRNA
                     GVKYLTLYAFSVENWVRPRAEIRGLMMLLKRFLSRQEYELHENKVRLRVIGRLADLPP
                     EIQRELKRVMKATERYDAGQLILALSYGGRAELSDAVRRIARRVRDGEIEPGDVDEKA
                     IAAHLYAPDVPDPDLMIRTSGELRISNFLLWQLSYSELYFTDVLWPDFREPQFREALA
                     EYARRHRRFGDIE"
     gene            3165..4010
                     /locus_tag="PK634_04255"
     CDS             3165..4010
                     /locus_tag="PK634_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOW96980.1"
                     /translation="MSFDKLKYRLPTGLAISAALFSAAWWLPFHGWLVVLLAITAVAL
                     WEFYGLLQAARIPHFPFVGIAGGLALILGTGVLQQAPGRVEIMVLFATLLAVLLRQFP
                     QKDNPRPLETIAGTLFGVLYVAFLFNFFTRLLVDWGDHDGRLLALYAIVVTKLSDVGA
                     FFVGCAIGRHKLIPRISPAKSWEGCFGGIALSVLGSLAAAALARPHWHQVSLGAGDAV
                     ALGLLLSLAGIAGDLTESLFKRAAGVKDSGRILLGMGGLLDVLDSLLFAVPMLYVYAT
                     FFLAG"
     gene            4034..5407
                     /gene="rseP"
                     /locus_tag="PK634_04260"
     CDS             4034..5407
                     /gene="rseP"
                     /locus_tag="PK634_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOW96981.1"
                     /translation="MLTLYTIVVIAFLFGITIFVHELGHFLVARWLGMVIETFSIGFG
                     PALWKRKVGDITYKVGLIPFGGYVALPQMDPTGEGKSEAGQTPVPPAPPGHRMLVALA
                     GVAGNMILAVLIAWIVYLGGQSYAPAEESGLVGYVDPESEVYAAGLRIGDAITAVNDQ
                     PVRSWDDFLVSSALYESPVLRVRAADGAEKSIPAATEKFMGARYVPGLLPMTHCWVLS
                     VDAGSSADKAGIKAGDRIVELDGVRLFSREHLMIQVAACRDRTVPAVIERQGAPMTLE
                     VTPAWDEKTEKVRIGVVFNTLDVKRPMAQIKSHALLIVRLLRALVTPGQSKAAAQAVG
                     GPVAIFGMFWLYVQGSFLMALWFTGLLNVNLAVLNILPIPILDGGHLLFALWEAVTRR
                     KISPRVFNVIMNVFASLLIALFILLVFRDTKRMILPLFRGDAAAEAATNGAPAAADEP
                     AGEPAGP"
     gene            5404..5838
                     /locus_tag="PK634_04265"
     CDS             5404..5838
                     /locus_tag="PK634_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96982.1"
                     /translation="MTQRNWAEGVWVPRWIVALAGVVVVMYVALHILVPPAWMIQRLD
                     SPDGRRTARLLRTRYAAKESLVVKARDRWGLRTLFYSPPLTNDFRVDLGERLYWSGDS
                     RRLFLEMEGRPVWGYDFSERRALAPEEIAGGTPGTRRAAPSP"
     gene            complement(5998..7671)
                     /locus_tag="PK634_04270"
     CDS             complement(5998..7671)
                     /locus_tag="PK634_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOW96983.1"
                     /translation="MKSSLVLVLVLVLVVLGAWLLWQWGFCRFYVEADEMAVIIAKIG
                     DPLPPGQILAKPGQKGVQEAVLGEGRHFLNPWLYERVIKPVIVIPPGKVGVVTSKVGD
                     DLPQDEFLAEPGQKGIWRGVLGPGKYRLNPYGYQVEIANAISIPVGYVGVITSLSGEQ
                     APPGQFAEAGQKGIRKDILQPGLYYINPKALKTDVLEIGVNQVSLIGKAGGEVITKTQ
                     IASQNVAMEQLQRQVLDEQKDKRISYAKRERAQEAVPAPMFEGRLVQQKMPDYTAALS
                     INQFVEFPSRDGFEISLDMTVEFELQPDLIASIFRSYGDLPAVVDKIIMPQILSVSRL
                     KGSAYRAKDFIVGEGREKFQSDLTESLARSLVERKIVVHNALIRHVNVPMQILDPIQQ
                     ASIAVEQDLTNQEKQNTAKKQAELNTQLSLIDQAREQVAQQTEKIKAEIRADQEKQVA
                     MLQADTLKQVADIERQTAQVRADRTRKLGGVEAKVIELVEGEKAKGFELKADAFGDPS
                     GFSLWEFAQSLNPQVQVNILHSGPGTLWTDLEKARLGDLGGAAAIQKEK"
     gene            complement(7668..9203)
                     /locus_tag="PK634_04275"
     CDS             complement(7668..9203)
                     /locus_tag="PK634_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOW96984.1"
                     /translation="MSDDSFPVPVHGRPKPRGAALPVFAGGGALLVIALVLALPLWIW
                     FFWRIEPAAGEIAILIHKTGADLPSGQILALEPGQKGIQLEVLPEGRYFRNPYSWGWK
                     IAAMTDIPAGKLGVLTRLYGADLPPGEIVAGEGTKGILADVLRPGKYRINPYAYDVEH
                     FDAISIRPGSVGVVTSLAGRDVLNSELKPEERNTFLVGAGLKGVVPEVLEPGTYYLNP
                     YLFAVSEVNLQSQRFEMSGDDAITFLTVDGFTVNVEGTLEYAVIREKAALITHRVGDM
                     DDILKKVILPRARGFSRIEGSKHPAINFIVGETRQQFQDNLEAHLRDKCADWGVAIKS
                     VLIRNIKPPDEIASVIREREVAVQNARKFDQQIEQAKSKAELGRQEMLAEQNKLKVEA
                     DTVRIRAVIRAQQDMAVRLTAARKDLEVAQVENEAADFQVQARLSGAEAERDVIKLNN
                     EAQAGVLRGQVKAFRTGMNFARYTLYRKLAPRIATILTGDQKDGLGAVFLPFLPDGKE
                     VSQ"
     gene            complement(9290..9925)
                     /locus_tag="PK634_04280"
     CDS             complement(9290..9925)
                     /locus_tag="PK634_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96985.1"
                     /translation="MQKRIRSVACFMVFLMGFGTWAAVVPYAKRLYPAGDVAAFSTLT
                     VPAGKVVVFDHMQFTSRTVSILFKINTAGSGIDSISGAYYMYITNPSPGQLTATPAPI
                     RLMGGWAISNLSSTAITFFGLAMDDDDLYAGVGNEIDSLNLAGGTLLGGIQLDSPRPV
                     VAVVEQSEDLKTWTEDADAAVQKGDAPAQLEFTTTPADAGAAFRVKARALP"
     gene            10067..11797
                     /locus_tag="PK634_04285"
     CDS             10067..11797
                     /locus_tag="PK634_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOW96986.1"
                     /translation="MKRRPPASKPKVRVPDWAADAIWYQVFPERFRSGCPASDPRPED
                     MDARPVPGWKVSPWGMDWYAQADWEKARGEFYRSVFHRRFGGDLVGLREKLDYLQDLG
                     VNAIYLNPIFMAPSLHKYDAACLHHVDPTFGPDREGDLALIAAANETEDPRTWIWTAA
                     DRYLADLVADVHRRGMRIILDGVFNHVGTHFFGFRDLVKNKRNSRYRDWFRIKEWRAD
                     GTFDYQGWFNHAGLPELARSKTTLAKPVRDYIFAITRRWMDPDGDGDPADGVDGWRLD
                     VAFCVPHGFWKSWARHVKKINPQAYTTGEVVGYADEFLRGDEFDAVMNYMWLYPTLGF
                     FSPSPDALPASELRAKLDELWRRYPRAVNYALQNLLDSHDTGRVLTFLENACPPFRDW
                     DSYFNYPKARENPGLKTTRPGPKAMDALRQMIVFQMTCLGAPMLYYGTEVGMWGANDP
                     DNRQPMLWDDIEHAPETRTLTGRCPSNPRKPDRDLFAFTRRAIALRKERHVLRRGRLR
                     WLDTGSERLLAFERFDRKTSIRVVLNAGDAPAEIRLEKPARDLWTGKTARRGRLALPP
                     RGWAVLEIQG"
     gene            complement(11820..12338)
                     /locus_tag="PK634_04290"
     CDS             complement(11820..12338)
                     /locus_tag="PK634_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96987.1"
                     /translation="MPERYKPATQGRLDNAEQDPALEIALKADTPVISIGEPLYLDVA
                     IRNVSEERVWLPRQPTVLVSWIYPNGVHDNFLKEIREEEHLERSELVCLEPGQQIAQR
                     VEIRTYYFDRPGITEFRARLYGARNTNPKTAETWTGRLQSNSFGVMVERAKKKMSDYR
                     IVTGACPDGPGA"
     gene            12583..12915
                     /gene="cutA"
                     /locus_tag="PK634_04295"
     CDS             12583..12915
                     /gene="cutA"
                     /locus_tag="PK634_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOW96988.1"
                     /translation="MKTWMIYFTAGTAAEAGKIGRALVRDRLAACVNILGRIRSIYRW
                     KGRVEDGREVAALAKTSEPRLPAAIAAIRKRHSYEVPCIVAWPLERGHAPFMKWIADE
                     TAVRTDSR"
     gene            complement(13039..15147)
                     /gene="glgX"
                     /locus_tag="PK634_04300"
     CDS             complement(13039..15147)
                     /gene="glgX"
                     /locus_tag="PK634_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOW96989.1"
                     /translation="MKKQTVQHLLPSTPLLVPYGAHVVEKGVQFTLFSRHASRVWLML
                     FDNPDDAEPAEELELSPDRNRIGDIWHLHVEDAREGQFYLYRMDGRTPRGVANFYDPD
                     QWLLDPYALAVAGAPKWGDARGLKAGETPKDGPLFPKGVIVRDDFDWTEDRTLRIPLA
                     DTVMYEMHLRGYTAHPSSGVGAPGTYRGLIEKIPHLRDLGVTAVELLPIQEFNEMEYY
                     QADDPRRDLRNFWGYSTLAFFAPNGRYARDGVYGRQVREFKQLVMALHQAGIEVILDV
                     VFNHTAENGDGGPTYSFRGIDNAIFYLMEEGRPHYRNFTGCGNTVNSNHPVVRDFIMN
                     CLRYWVLHMHVDGFRFDLASILTRGADGELLPDPPIVEHIAEDPALRDTKIIAEAWDA
                     AGAYQVGTFPNERWSEWNGKYRDEIRRYWKGDPGMLGQLAMRLTGSADLYDKLGQTPM
                     KSINFITCHDGFTLRDLVSYDGKHNEANGEKNRDGENHNHGSGYGVEGPTDDPDILAV
                     RRRQQKNLLTTLFLSMGVPMVLAGDEFSRTQQGNNNAYCQDNEISWVDWSLLEANRDL
                     HAFVKKLIAFRKAHPSLRRTRFLNGKKPEATNADITWHGINGRAPDWEKDCAIACVLN
                     GRSEHTGGPEDDDSLFMIFNAGTEPATFMVPPPPDKPWALALTTEENPPMWYRVKGVF
                     TADARSTSVLASAPFRRFWR"
     gene            complement(15275..16519)
                     /locus_tag="PK634_04305"
     CDS             complement(15275..16519)
                     /locus_tag="PK634_04305"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HOW96990.1"
                     /translation="MISTVLKLMVRLAVILAAILVALFAGELLRFYILLYRIRPLFAD
                     IYSWIVVLILVSVLVYILYLLRRHPPIPQPPRVTGREPDHVRMRKYCRYLVDYLNHMA
                     RNPNLGADRQSVLRQGAGEIREVLGAHPLNEDLARLILKTEEQVVLPALAELNALASA
                     EVRHSVRDVMMGVTLSPYQALDLIMVSYRNLAMAGRIVRLYHGRPAPGDSWRILRDVL
                     VTVATVNALGLTRKLFASLLSEVPLVGRVVEGIGQGLGAGLMTSITGHAAMDRCMSFR
                     GWNAEEERRSLGERMAGFLADVRDLFTKDLLPQLKGLIRSGAQPGAADQPGFWDSIAH
                     GIGSAVDLTARMADTLVVQPVVAVGAGVVQAGTYLTRGAVQAGQTAVRVTRRRVRHAS
                     TGAGRVLRTFGQRLYYLFRGPR"
     gene            16777..17763
                     /locus_tag="PK634_04310"
     CDS             16777..17763
                     /locus_tag="PK634_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOW96991.1"
                     /translation="MDELKIHFDNAREAQDVTGKRLEYLARIEDALGVRLTARDTWLL
                     AEGDPAKAAAALRFVDILRRARSAGISLREHTVMFALRAFREGREQALTQLHACRIDV
                     SPGKAPVFPRTFGQHAYVEAIRGTDISFGIGPAGTGKTYLAMAMAVSTLLRNEVSRII
                     LTRPAVEAGEALGFLPGDMHQKVLPYLRPLYDALYDMMTPDDIERHMVRGIIEVAPLA
                     YMRGRTLNHAFVILDEAQNTTPEQMLMFLTRLGFDSKCVITGDLTQVDLPPYKTSGLL
                     EARRTLRAVEGISIIELSDADVVRHELVQKIIQAYRESREAAAPPAEQESAK"
     gene            17763..20126
                     /locus_tag="PK634_04315"
     CDS             17763..20126
                     /locus_tag="PK634_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOW96992.1"
                     /translation="MILPWSRGRAFRRAPLLAARKTARAPVPTLPLLLGLLLWVSALV
                     VLNLTPDRPYSGLTPGQRAPVTVVAQVDFKCEDLARTEMNRQQAANAVPPAFTIDATP
                     LNTAARALDKLVDRLMAERERQAGLPAPDPEGLVRALGNALDLLGLPLTPDEVQALFP
                     AGAEAGARDAIKKAAREVWGHGIISPDEKASAFQGLASTGAIGVQAADGAWRSPLPLD
                     ELPLPAAAVDAMVKRIPEKVGENALPREALRALLKPWVGPNLAYDPRTTAEQRRAAGV
                     AVEPAIVRVTAGTMLVEAGEPVTPQVLEYLQKHERRRRQLESPYDLLLKQIGNAGLLL
                     GALIAGAGLLHVLSPALLQNPRLLLLLIVISLVSLGPTRFAVNVAGRTGLLSPALIEF
                     VLPLALSPLLAVILLGVPAALMVGVWNSLVMAILFDQSFSMLLLGLFVTAVATLSARD
                     VRQRSRIYRAGVLIGLAEVLAVIAFAAVRGLPRTALLIQAGTGLAGGMVYALLAILLI
                     PLLEWAFRVTTNLTLLELCDLSHPLLQRLAMEAPGTYHHSIVVANLAQEAAQKIGANT
                     LLVRAAAYFHDIGKLAKPEFFIENMRPGVNPHDTLAPRMSSLIITSHVKEGLGLARRY
                     RIPASVAAGVEQHHGNGLVSYFYHRARQQKEEGEANESAVPDQEYRYGGRRPQTREMA
                     ILALADSVEAAARALEDPTPARIENLVHDIAAGKLQDGQFDECPLTLGELATIRRSFV
                     FSLANMLHVRIAYPPDETKPPQPAEPDLSEDGEPEESDPVAGGPDRP"
     gene            20029..20499
                     /locus_tag="PK634_04320"
     CDS             20029..20499
                     /locus_tag="PK634_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HOW96993.1"
                     /translation="MKLSLHNRQSRISLKTANLKKATRWLADRIARDSGLAPWSELAV
                     TFTDDEGIVPVNAACFGKAWPTDVITQPYISPPGGDVIVNVERALAVGGAGPRGEAEL
                     ALYIAHGLHHLAGAVDDTPARRRAMLRQEQAWLRAAENEGLLAALWRAAGPGRR"
     gene            complement(20501..21646)
                     /gene="amrS"
                     /locus_tag="PK634_04325"
     CDS             complement(20501..21646)
                     /gene="amrS"
                     /locus_tag="PK634_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOW96994.1"
                     /translation="MTARYTRRQFLGRCACGGLGAALGAAIPCPAFAQDRRAARWAAA
                     GDLREAAFWEPAGDGRTRCLTCPNECVCAEGGVTACRTRINLGGRLYSMTYGRPCVVF
                     QDALEKNPLYHVTPGQEALGIGTAGCNLRCLYCQNWEFSQYGPWETRNMSLAPDALVE
                     RAVSRGLKWITFSYTEPVAYLEYALDVARLAKQRGLRCAVVTGGFIHPKPLAALLECG
                     AAFSVTLKGADEQFYRDVVACPRDSVWQTIRSIAQSGRWIEVVNLIVPTMNDQPEGIR
                     AIARSLAGLNPDIPLHFLRFAPAYKLKHLPPTPRETLEQARDIAMKEGLRFVYLANLP
                     GHEGASTVCPACRRTLIERVGFKVLRNDIRGGRCPACQAKVPGVDLG"
     gene            complement(21651..23330)
                     /locus_tag="PK634_04330"
     CDS             complement(21651..23330)
                     /locus_tag="PK634_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW96995.1"
                     /translation="MKMQAAVRLLAGALALVGATARAGNGSDFLQLERDIPSLYLLNG
                     LFLTPGQNAGLAARLSEMERMETEAAARVESLVRRYGDADALARLAGRNGGEGRMARP
                     PPELKDIQAQREERERQVRGLAREVYGLLTPAQVEIIDRFTPCFIPPGDFRDPVRVGQ
                     AENHLGVGEPILKRLREVPEFRLDEARDRATEALTRYVLQKRRVKYSEEAEAGVRKEI
                     ETALDQELPRIRSLSDVDLELEKEDILCRLIPLEERPASTDEQLKLIGLYVLNPGCLD
                     VVRKRANTPVPAVSPREASAPRPADTLGSAQRLKAVRLLAGLDLTEGQADRLLPVARS
                     ARDARAEVHARAEEVKAKALTDYRQLRAELADGNPTRETEQACQRWHQAVKTIYEQDL
                     FAALRPYEEQAEQVLTANQSAMFAAAPNPPSPDQGGPMPARRQHHLALRTLEEARAMS
                     PAQFQRDGADLADRFVRECVGQEPDMGVDIPTESERAQDVLEKARGMSQGAFRRQLRD
                     LVAEMSPRRSAPRETAYGTKYDRGEPLPVLGPGAELLFTDAGVELLSKLAR"
     gene            23728..24450
                     /locus_tag="PK634_04335"
     CDS             23728..24450
                     /locus_tag="PK634_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOW96996.1"
                     /translation="MFRKILKALRNNILTGLFLVTPIAVAVFVVLWLFTLITDRIMPF
                     VPRKVGEVYPEILFRLIALLIFLAVLFLLGLLIRNMIGARLYRIGDAILSRIPVFNKI
                     YISVRQVGEALLAQSQTLFQEVVLVEYPRKGLFSMGFVTTPAPPEFIRNLPDGVATPL
                     VAVFIPTTPNPTSGLFIFVPKSETYKMPIGVGDAMKLVVSGGAVYPGAPGRADSRPTF
                     LDKLQNWAEREGRDTAPGAGPS"
     gene            24447..25217
                     /gene="recO"
                     /locus_tag="PK634_04340"
     CDS             24447..25217
                     /gene="recO"
                     /locus_tag="PK634_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOW96997.1"
                     /translation="MILKTTALALRLYPFSNTSRIVSWLTPDQGRIVTMIKGSQRARS
                     LFLGQYDLFYTCELLYHRRERGGLHIARECSPLKWRAGLRHDWKAAATASYLAGLVAQ
                     AVPPEAPHESLFRLLDSALDHLEAAGAGEPFVFWFELRLLEALGLSPRLRNCAACGAE
                     LASSSRRSDFAAARGGLLCAGCVDKTSSGPRVGGDVLGMLAGWQQARLPQGPLNTKCS
                     ARQIQAIEALLSGFLEYHLDVRPVGRREALDVLARRVA"
     gene            complement(25231..25722)
                     /locus_tag="PK634_04345"
     CDS             complement(25231..25722)
                     /locus_tag="PK634_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOW96998.1"
                     /translation="MAKKKGPRHILWAPWRMEYIKRPRESGCFLCNSLRSRADRANGV
                     VLRGRTCAVVLNRYPYTGGHLMVAPLRHVAALDGLTKPELNEMIRLMMRSLGLLRKAL
                     HPHGFNVGLNIGEAAGAGLKDHVHLHIVPRWNGDTNFMPVLGKTQVTPVSLGSLWDLL
                     RGK"
     gene            25872..27584
                     /locus_tag="PK634_04350"
     CDS             25872..27584
                     /locus_tag="PK634_04350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOW96999.1"
                     /translation="MAKEPEKKVLAGRYEIRRPLGAGGVGMVYQAWDLQLHRFVAVKR
                     WRPPDDLVEDSAGTEKLWREAMTLAAIQHPNILTIHDFGVDPEGPYVIMEYVDGETLD
                     RVIQRGPFDRDSFAEASQQILEALIAAHQAGMIHRDLKPQNIMRTRLPSGGWQYKILD
                     FGLARFVTQPTVQSMEGNTSIYGSIFFIAPEQLKHQPLDARTDIYAIGCVCYYMLAGR
                     YPFEGDSIPGVITSHLEHNVKPLGDWRPDLPPSLCNWVMKALSFSPDERWGSAAEALT
                     ALRRILPGTVRTTTIKIVTPVRGPQITPLGAESVPPTTGMLKISPVKKPEGAAESPAG
                     PGLLRRALVYMVPLALLAAIVTALVWLRAPPAPPEAIPAEPAADSAASTEITAWPPYI
                     PEERRALVQLYFKQLRDLQGFKWASIGTVMEALAAVQFKEQFDPSQMQALPNISYHDE
                     TGRTVGELDVVLWNVPSNCAEVVYEAVITDNLRRNKASSWTQLNRFRVAVQERTVHHF
                     IYAHDPTWKMDISQFDHARYGLMGSRGALAEGYESEVDITRAEAEFLQRRIIEYQKNR
                     HAGP"
     gene            27890..29266
                     /gene="amt"
                     /locus_tag="PK634_04355"
     CDS             27890..29266
                     /gene="amt"
                     /locus_tag="PK634_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOW97000.1"
                     /translation="MLAGWAGVARAQEAGPVFTQEMADTVANYKITLDTLWVLITACL
                     VFFMNLGFAMVESGLCRAKNTVNILAKNFIVFGIASLAYYILGWGLMFGDGSGFAGLK
                     GLWFAAGADNSPAAGDAYQGVYSAISWATVPFWAKFFFQLVFAGTAATIVSGAVAERI
                     KFHSFMLFSFILVAILYPITGHWIWGGGWLQRLGFWDFAGSTAVHSVGGWAALAGVLL
                     LGPRLGKYRPDGKPRPVFGHNMSTTALGVFVLWFGWFGFNPGSTMAAAAGDIARIAVT
                     TNSAAAAASLSATIMAWRLLGKPDLSMILNGCLAGLVAVTAPCAFVSVPISIVIGLIA
                     GVLVVLAVLFFDRMKIDDPVGALSVHLVNGVFGTLAVGLFAQADIMPNTTGNGLFFGG
                     GFALLGHQFLGVLAVGGFTFAVALAVWAVIKATLGLRVSEEEELRGLDIGEHGMEAYS
                     GFQVFTTQ"
     gene            29292..29633
                     /locus_tag="PK634_04360"
     CDS             29292..29633
                     /locus_tag="PK634_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOW97001.1"
                     /translation="MKLIIAYIQPERLNAVKQALYEAEVFKMSVTNALGCGQQKGYHE
                     SYRGADVEVNLLKKVRLEIAVNDAFVKPTLDAIIKGARTGRIGDGKIFVMPLEECYRI
                     RTGETGSPAIG"
     gene            complement(29689..31599)
                     /locus_tag="PK634_04365"
     CDS             complement(29689..31599)
                     /locus_tag="PK634_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97002.1"
                     /translation="MSPGKRILFRLLALLLPLLALATIEAGLRLSGRGGYPAFLREVG
                     RLPSGDMLCLVEPAASKPYFFANPDRPGYAEQSTFVMPKPADVVRIFIIGESAAKGYP
                     QPRNLSMAAFLQEILNSAWTDRKAEVISLGTTAVASFPLVYLVRDALKFEPDLLIFYT
                     GNNEFFGAYGTGSINAAGTLPPSALRVLRALRGLALVQALDTWRGRQPDEGVTLMEEM
                     IGRSAIHANSPLREAAARNLAANLGAMLDAAKAAGVPAIVCTTAANESDLAPLGADNE
                     ARARYRRGREQAAAGDRDGARRSFLEARDRDPLPWRPTSQTEEAIRSAAREKGAGLCD
                     IAELFRTESPAGATGRDLVEDHVHLSLRGQARAARAMAEAMGSLPGALRVDPGALAGL
                     PDDAELARRLGANPYDEYRVNHTLRVLYGVPFMKRANPELFARHNTACREAEARMSPG
                     VLAAAREWQTARPHAGGLRPITAMIARALLREGRPAEALPLYEIAARQVPEYTSWHLE
                     YVYFTLACREKLAGGLSEDERAAAARAIAQGVFLLAHGASKTGLTERYVGRLCQLRGE
                     WAEAIPYLLAARPSMDAEDLVACNQALVVSYMKTGKTAEALALADDGIRAGGRFAGIY
                     RKLRDEIERAAR"
     gene            complement(31624..32346)
                     /locus_tag="PK634_04370"
     CDS             complement(31624..32346)
                     /locus_tag="PK634_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW97003.1"
                     /translation="MKKVLFLASLVAAASVQASIIVNDSLSEVFVSANGTMDIVSMEV
                     SHTASDLIFTLAVNGDVTNPDWGNFMIGIANMKDAGSTTGNGWNRPINLDAGSGYGMT
                     HWLGSWVNSGGGAQLWTYSVGAGGGTGGNWSGPAALAAYSFVSGPTSTVTMTVTRSSL
                     GLTGNDTFYFDAYSSGGGGTDSAVDALSNPNQTISDWPNPYTSSGTTGLSTYTIPEPS
                     TAMLMGVGALVGLGVFRRIRRA"
     gene            complement(32471..33040)
                     /locus_tag="PK634_04375"
     CDS             complement(32471..33040)
                     /locus_tag="PK634_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97004.1"
                     /translation="MKKFAQFVVLISGFCLLSPVAGGQEPPPPGEPPLPSEQETLAFL
                     ETNLPEVFENLQRLRQNEPEIFQHEIRMLGHMVRRYNELKRVAPELAAGFLRAQQLDA
                     QCRKVAEALRQAPDEAQKTALKQKLEEMLNEIFDLRLAERELEVKNLERELNKIRTML
                     ETRREAKGQIVERRLRDLTMEVDEAMGWW"
     gene            complement(33037..33804)
                     /locus_tag="PK634_04380"
     CDS             complement(33037..33804)
                     /locus_tag="PK634_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97005.1"
                     /translation="MKISHEELLLYAGGELTPDRRARVEEALRSDPEARKALAEFQEQ
                     EARLARLPVLEPGRDLVAPALTAARRRKTARVLFLYPAWRAAAAALLLLLGGAAWRLW
                     RRAPGIQVARDTTHPPAGLVSDDELAVRLSQLRAAVGGLAPAAPAPALEPEETFAADV
                     KERLETLQGLCRSQVAATRPARPGADFDRRMQQLKSQMDTAREDLLKIEIPADDEIAG
                     RGAPTIQRNTTIRVSLRGYPPLKGPAARPVCQERSPS"
     gene            complement(33801..34391)
                     /locus_tag="PK634_04385"
     CDS             complement(33801..34391)
                     /locus_tag="PK634_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW97006.1"
                     /translation="MAEVSHSGPDDRLDTDLIRLWRGGHESALEDLLRRYQARIFSFL
                     CRMLRNRHDAEDAAQEAFIRAATKLHRFDEAKGTFKSWMFQIAYREGLRMAKKRRRLP
                     IGESAWTEHDVPAPEAVDPGPSPGRQLEEQETAARVAQAVATLPEAEKQVVLLRTYSG
                     LRFREIAEVMKTPLNTTLGRMHNASQRLRKWFGEAP"
     gene            34537..36498
                     /locus_tag="PK634_04390"
     CDS             34537..36498
                     /locus_tag="PK634_04390"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOW97007.1"
                     /translation="MNEAKSFAGEWNMAVRAMMVHAALSVGVLNLVPGVAAGATYYVR
                     PDGGSAQQCNGLVDAPYPGSGEGQPCAWDHPFRALPPGGAPNIAGGDTLIIGPGSYKM
                     GYGAPGADACNSGWPWECAMPPVPSGPDPQHPTRILGASWASGCTNKPELWGAERATF
                     IFNLTGSSNVEIGCLELTDHEGCVEFHSGGLPCERDSFPYGDWCQRGLYAEDARNVYL
                     HDLDIHGMADTGIQAGRLADWTVEDVRLAGNGWSGWDGDIAGDDGNTGTLFFSGFVVE
                     WNGCGETWPGGQATGCWAQTAGGYGDGFGTGRTGGNWIFVDCAFRHNTSDGLDLLYVR
                     EPGSSILISRTLAEGNAGNQLKTSGPAQIENCVVVGSCAFFDGQPFTFNVDNCRAGGN
                     AVSLSLAPTNQASILNCTITSEGDCLVIAGCAGTNCNGSESVYLRNNVFLGHTDFFQP
                     WENTCLTYDEGFPATPFDLDYSVISGVKNDPCPVGPHDLCASPQLAGMTLAAFDARLL
                     SNSPAMDAGTNRPAVGGDFDGVSRPLDGNHDGTNRHDIGALEYAHALADSDGDGMADA
                     DEVTAGTDATNSVALFTMEILRAVPDPVLGWSGVAGRAYDLFSAPAVTGAWVAVEGGT
                     NLPGRDAPQWFTNAAPDERRFYRLGVRVR"
     gene            complement(36520..36693)
                     /locus_tag="PK634_04395"
     CDS             complement(36520..36693)
                     /locus_tag="PK634_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97008.1"
                     /translation="MKMRNLGRWNRIQARFIARLNLSRDPQALLAFASWFAYCVDHRG
                     RLPRLADAAQWKT"
     gene            36869..>37872
                     /locus_tag="PK634_04400"
     CDS             36869..>37872
                     /locus_tag="PK634_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97009.1"
                     /translation="MKKIVCWAVCGWMASAAHGMVRLNDGGLYLSIQGAVNAATNGDT
                     LKIAADFYPEVVIVSNKNLTLEGGYNGAFTARDGGDSVVDAQQAGTTLWFIDSSSRVD
                     RVSLTGGTGHPQNLWCGGGSLLHRSWIEFTDCRVYSNAATFGGGLFVGLDAYAKLTGA
                     TEIYSNAAVWSGGGAFVDGRCDLVHSNTLVYGNTANEGSGGGIWVETGYLRLFQGWVR
                     DNLALPGGSGAAAAGGGVGASGSFVEIGDGAALMQNEAVLGGGLALFNSTGHLGRARI
                     ADCYLGVNSAVERGGGLYASNSVLLAKGLCCWGNDAILAGGGAWLHSCTVNSDTNGMG
                     A"
BASE COUNT         5747 a        12715 c        13080 g         6320 t
ORIGIN      
        1 agacgtcgaa caggtcgccg cccagcatat cgcaggtgag gtagtagcgg tcgaagacga
       61 tcttgtcctg ccgggggaag ctcttcggca ggaagccgag ctgcacggac tgggcgagct
      121 tcatctcgcg cacgaactgc tcgttgttgt gggccagctc cgtgttggcc gcgcgcagca
      181 tctcgttgcg gcccgcgagc tccgccgcca gcgcccggtt ggtctccagg gcttcctgct
      241 cgcggtagaa cagcgagacc accatgatca tcagcagcgt cagcacgacc cacacggtgg
      301 cgatgacgat ctgcagggcc aggagcttgg cgcgcaccgt ggtggccgtg atgtcagcgc
      361 cgacgatggc gaccgtctgc cctcgctcgt tgtggaccgg cgcgtagccg gacatcaggt
      421 ccgggtaggg cgggtcgggg gacacctcgg ggtccgccat cggctggcgc caggcggcga
      481 ccagttccgg caggtgccgg gccgagtagg ccttgccggg cagttcgctc tcttcgtccg
      541 cggcgatcac gccgtcctcg tcgatgtcgc gggcggggcc gtccacgacg aactcgtagt
      601 cgtcctcgcg ggcgtcctcg cgcagggcgc gccgcatcgt gtagatatag cggatgtccg
      661 agttggagga gacgacgcgg cccaggtaat gctggacgcg ctggtagggc ggcgtcccga
      721 tgtgctcggg cttgtgaacc tgttcgaggt ccgcgggatc gaggctctcg gccaccgcga
      781 tggcgatgcc catggcctgg tggcggatct cgttgatcac cgagcgccgt gcgctgatgt
      841 agaggagcgc gaacaacagc aggacgatca ggctggcggc cgccaggccc gtccaccgcc
      901 gatggctgac gccccccaac agcggggtcg gctcctcgct gcccgccatc gtttgtgtgc
      961 ggccgtccat cgcaggattt cccggctgac ctttcaaggc taacataatg cacctgccct
     1021 cgggatgtgt atagaaaaaa cgaaacccgt tgcgccgccc ccccttttcc gttgcaccgg
     1081 ccgggggcat tggatacgtt acagcacccg ggcccccgcg tggagcgccc gcgcaaacaa
     1141 ggcatgccat gtcaagcacg gtattgatcg gggcgcagtg gggcgacgag ggcaagggca
     1201 agatcatcga tgtcctctcc gctcgcgccg attgggtggt ccgctaccag ggcggcagca
     1261 acgccggcca caccgtcgag atcggcgagc agcggttcgt gctgcacctg atcccctccg
     1321 ggctcctcca ccccgcctgc cgggggatca tcgggaacgg cgtcgtcgtg gacccggtcg
     1381 ccctgctgaa ggagatcgac ggcctgcgcg agcggggcat cgagcccgcc ggccgcctct
     1441 tcgtcagcga ccgcgcccac gtggtgctgc cgttccaccg cctgctcgac gcccggcgcg
     1501 agcagaaccg cgccggcggc acgctgatcg gcacgaccaa gcgcggcatc gggccggcct
     1561 acggcgacaa ggcctcgcgg gtcggcctgc gcatggccga cctggtcgcc ccggacctgg
     1621 ccgacctgct gcggccccgc ctcgcggagc agaaccggat cctcgaggcc ctcggcgcgc
     1681 ccgcgatgga caccgccgcc gtcgtcgcgg agtaccaagc cgcggccgcg cgcctggcgc
     1741 cgttcgtggc ggacacgatc ccgatgctcc acgcggccgt cgcgcgcggc gagcgcatcc
     1801 tcttcgaagg cgcccagggc acgatgctgg acatcgattt cggaacctat cccttcgtga
     1861 cctcctccaa cgccaccgcc ggcggcgcgt gcgccggcag cggcgtgccg ccccaccgca
     1921 tcgggcaggt cgtcggcgtg gtcaaggcct acacgacccg cgtgggcgag gggccgttcc
     1981 cgacggagct gaaggacgag accggggcgc ggctgcgcga ggaggggcgc gagttcgggg
     2041 cgaccaccgg ccggccccgc cgctgcggct ggttcgacgc cgtcgtcgcc cgccacgcgg
     2101 tgatgatcaa cggcatcgac tggtgggccg tcaccaagct cgacgtgctg gacgccctgg
     2161 aaaccctccg gatctgcacg gcctacgagt gcgacgggcg ccggctggag cacgtgccgg
     2221 ccgacgtgcg cgtgctggag cgctgccgcc cggtgtacga ggacttccgc ggctggcggt
     2281 cctccacccg cgaggcgacg cggttcgagg accttccgga acgcgcgcgg gcctacctgg
     2341 cgcggctcga ggagttgacc ggcgcgcccg tgggcatcct gtccgtcggg ccgcgccgcg
     2401 agagcacgct gtggctcaag gcggcggggg agggcgcgtg nnnnnnnnnn gaaggaagag
     2461 gccgccccga ggatccccgt ccacgtggcc atcatcatgg acggcaacgg ccgctgggcg
     2521 aagcggcgcg gcctgccgcg cctgaagggg caccaggagg gcgcggaatc cgtccgggcg
     2581 atcatccgcg cctgccggaa cgccggggtc aagtacctga ccttgtacgc gttcagcgtg
     2641 gagaactggg tccggccgcg cgccgagatc cgcgggctga tgatgctcct gaagcggttc
     2701 ctctcgcgac aggaatacga actgcacgag aacaaggtcc ggctgcgcgt gatcgggcgc
     2761 ctggccgacc tcccgcccga gatccagcgc gagctcaagc gggtcatgaa ggcgaccgag
     2821 cgctacgacg ccgggcagct catcctggcg ttgagctacg gcgggcgcgc ggaattgagc
     2881 gatgcggtcc gccgcatcgc gcgccgcgtc cgggacggcg agatcgagcc gggcgacgtg
     2941 gacgagaagg ccatcgccgc ccacctctac gcgccggacg tgccggaccc ggacctgatg
     3001 atccggacca gcggcgagtt gcggatcagc aacttcctgc tttggcagct ttcgtacagc
     3061 gagctatact tcaccgacgt cttgtggcct gacttccgcg agccgcagtt ccgcgaggcg
     3121 ctggccgagt acgcgcggcg gcaccggcga ttcggcgaca tcgaatgagt ttcgacaaac
     3181 tgaaataccg gctgcccacg gggctggcga tcagcgcggc gctgttctcg gcggcgtggt
     3241 ggctgccgtt ccacggctgg ctggtcgtgc tgctggcgat cacggccgtc gcgctctggg
     3301 agttctacgg cctgctccag gccgcccgca tcccgcactt cccgttcgtc ggcatcgccg
     3361 gcggcctggc cctgatcctc ggcacgggcg tcctgcagca ggccccgggc cgggtcgaaa
     3421 tcatggtcct cttcgccacg ctgctggcgg tgctcctgcg gcagttcccg cagaaggaca
     3481 acccccggcc cctcgagacc atcgcgggca cgctgttcgg cgtgctctac gtcgcgttcc
     3541 tcttcaactt cttcacgcgg ctgctggtgg actggggcga ccacgacggg cggctcctgg
     3601 ccctctacgc gatcgtcgtc accaagctct ccgacgtcgg cgcgttcttc gtcggctgcg
     3661 ccatcggccg gcacaagctg atcccgcgga tctcgcccgc gaagtcctgg gagggctgct
     3721 tcgggggcat cgcgctgtcc gtgctcggca gcctggccgc ggccgccctc gcgcgccccc
     3781 actggcacca ggtctccctg ggcgccggcg acgcggtcgc gctgggcctg ctgctgtcgc
     3841 tcgcgggcat cgcgggcgac ctgaccgagt cgctcttcaa gcgcgccgcc ggcgtcaagg
     3901 actccggccg catcctcctc ggcatgggcg gcctgctcga cgtgctggac agccttctct
     3961 tcgccgtccc catgctctac gtctacgcca cgtttttcct cgccggatga tccgccacca
     4021 aaggaagtgc tgaatgctca ccctctacac aatcgtcgtc atcgcgttcc tgttcgggat
     4081 cacgattttt gttcacgaac tcggccactt cctcgtcgcc cgctggctgg gcatggtcat
     4141 cgagaccttc tccatcgggt tcgggccggc gctctggaag cggaaggtcg gggacatcac
     4201 gtacaaggtc gggctgatcc cgttcggcgg ctacgtggcc ctgccccaga tggacccgac
     4261 gggcgagggc aagtccgagg cgggccaaac gcccgtcccg cccgccccgc cgggccatcg
     4321 catgctggtg gcgcttgcgg gtgtcgcggg caacatgatc ctggcggtcc tcatcgcctg
     4381 gatcgtctac ctcggcggcc agtcctacgc gccggccgag gagagcggcc tcgtgggcta
     4441 cgtggacccg gagagcgagg tgtacgccgc cggcctgcgg atcggcgacg cgatcacggc
     4501 cgtcaacgac cagcccgtgc gctcgtggga cgatttcctg gtcagcagcg ccctgtacga
     4561 gagcccggtc ctgcgcgttc gcgccgccga cggcgccgag aagagcatcc cggcggccac
     4621 ggagaagttc atgggcgcgc ggtacgtccc cggcctgctg ccgatgaccc actgctgggt
     4681 cttgagcgtc gacgcggggt cgagcgccga caaggccggg atcaaggcgg gcgatcggat
     4741 cgtcgaactc gacggcgtcc gcctcttcag ccgcgagcac ctgatgatcc aggtggccgc
     4801 gtgccgggac cggaccgtgc ccgcggtgat cgagcgccag ggcgcgccga tgacgctgga
     4861 ggtgacgccg gcctgggatg agaagacgga gaaggtgcgc atcggcgtcg tcttcaacac
     4921 gctggacgtc aagcggccga tggcccagat caagagccac gccctcctga tcgtccgcct
     4981 gctgcgggcg ctcgtgacgc cggggcagtc gaaggcggcc gcgcaggccg tgggcgggcc
     5041 cgtcgcgatc ttcggcatgt tctggctcta cgtgcagggc agtttcctca tggccctctg
     5101 gttcaccggc ctgctcaacg tcaacctggc cgtgctcaac atcctgccga tcccgatcct
     5161 cgacggcggg cacctgctgt tcgccctgtg ggaggccgtc acccgccgga agatcagccc
     5221 gcgcgtcttt aacgtcatca tgaacgtgtt tgcgtcgctg ctgatcgcgt tgtttatcct
     5281 gcttgtcttc cgcgacacca agcggatgat cctcccgttg ttccgcggcg acgccgccgc
     5341 ggaggcggcc accaacgggg cccccgccgc ggcggacgaa ccggccgggg agccggccgg
     5401 gccatgaccc agcggaactg ggccgagggg gtgtgggtcc cgcgctggat cgtcgcgctg
     5461 gccggcgtgg tcgttgtgat gtacgtggcg ctgcacatcc tcgtgccgcc cgcgtggatg
     5521 atccagcgcc tggactcgcc ggacgggagg cgcaccgccc gcctgctgcg cacgcggtac
     5581 gccgcgaagg agagcctcgt cgtcaaggcg cgcgaccgat gggggttgcg gaccctgttc
     5641 tactcgcccc cgctcaccaa tgacttccgc gtggacctcg gggagcggct ctactggagc
     5701 ggggattcgc ggcggctctt cctggaaatg gaaggccgcc cggtctgggg ctacgacttc
     5761 agcgagcgcc gcgcgctggc gccggaagag attgcgggcg ggacgcccgg cacgcgccgc
     5821 gctgctccct cgccgtgaag gcgcgcacga cgaagacgct catctgtacc gcgcgggggc
     5881 tgttcagcgc ctttgtggaa accccgtaga tccgcgccat gtccgagtcc aggtcccgga
     5941 cggttcggat ccgggcttcg ggtgagacct ttcccctctc ctcgctcatg cggggcgcta
     6001 tttttccttc tggatcgccg ccgcgccgcc gaggtcgccc agccgggcct tctccaggtc
     6061 ggtccagagc gtgcccggcc cggagtgcag gatgttgacc tgcacctgcg ggttcaggct
     6121 ctgggcgaat tcccacaggg agaagccgga cgggtcgccg aacgcgtcgg ccttgagctc
     6181 gaagcccttc gccttctcgc cctcgacgag ctcgatcacc ttggcctcca cgccgcccag
     6241 cttgcgcgtg cggtcggccc ggacctgcgc ggtctgccgc tcgatgtcgg cgacctgctt
     6301 gagcgtgtcg gcctggagca tggcgacctg cttctcctgg tcggcccgga tctcggcctt
     6361 gatcttctcc gtctgctgcg cgacctgctc gcgggcctgg tcgatcaggc tcaactgggt
     6421 gttcaactcg gcctgcttct tggccgtgtt ctgcttctcc tggttggtca ggtcctgctc
     6481 gacggcgatg ctggcctgct ggatggggtc gaggatctgc atcggcacgt tgacgtgccg
     6541 gatcagggcg ttgtgcacca cgatcttgcg ctcgacgagc gaccgggcga gggactcggt
     6601 caggtcgctc tggaatttct cgcggccctc gccgacgatg aagtccttgg cccggtaggc
     6661 cgagcccttc agccgggaga cggagaggat ctgcggcatg atgatcttgt ccaccaccgc
     6721 ggggaggtcg ccgtagctgc ggaagatgga ggcgatcagg tcgggctgca gctcgaactc
     6781 gacggtcatg tcgaggctga tctcgaagcc gtcgcgcgac gggaactcga cgaactggtt
     6841 gatgctcaac gcggccgtgt aatcgggcat cttctgctgg acgagccgcc cctcgaacat
     6901 gggcgccggg acggcctcct gggcgcgctc ccgtttcgcg tacgagatcc gcttgtcctt
     6961 ctgctcgtcc aggacctgcc gctggagctg ctccatcgcg acgttctggc tggcgatctg
     7021 ggtcttggtg atcacctcgc cgccggcctt gccgatcagc gagacctggt tgacgccgat
     7081 ctccagcacg tcggtcttga gggccttggg gttgatgtag tagaggccgg gctggaggat
     7141 gtccttgcgg atgcccttct ggccggcctc ggcgaactgg cccggcgggg cctgctcgcc
     7201 ggacagcgac gtgatgacgc cgacgtagcc gacggggatg ctgatggcgt tggcgatctc
     7261 cacctggtag ccgtacgggt tcaggcggta cttgcccgga ccgaggacgc cgcgccagat
     7321 gcccttctgg ccgggctcgg ccaggaactc gtcctgcgga aggtcgtcgc cgaccttcga
     7381 cgtgaccacg ccgaccttgc cgggcgggat cacgatgacc ggcttgatca cccgctcgta
     7441 cagccacggg ttcaggaaat gacggccctc gccgaggacc gcctcctgga cgcccttctg
     7501 gccgggcttg gcgaggatct gtccgggcgg cagcgggtcg ccgatcttgg cgatgatcac
     7561 cgccatctcg tccgcctcga cgtagaagcg gcagaagccc cactgccaga ggagccaggc
     7621 gccgaggacg acgagaacga ggacgagaac gagaacgagg gaggatttca ttgggacacc
     7681 tccttcccgt cgggaaggaa ggggaggaag acggcgccca gtccgtcctt ctggtctccg
     7741 gtcaggatcg tcgcgatgcg gggcgcaagc ttgcggtaga gcgtgtagcg ggcgaagttc
     7801 atgcccgtgc ggaaggcctt gacctggccc cggagcacgc cggcctgcgc ctcgttgttc
     7861 agcttgatca cgtcccgctc cgcctcggcc ccggacaggc gcgcctggac ctggaagtcc
     7921 gccgcttcgt tctcgacctg ggcgacctcg aggtccttgc gcgcggcggt caggcgcacg
     7981 gccatgtcct gctgcgcgcg gatgacggcg cggatgcgga ccgtgtcggc ctcgaccttc
     8041 agcttgttct gctccgcgag catctcctgg cggcccagct cggccttgga cttcgcctgc
     8101 tcgatctgct ggtcgaactt ccgcgcgttc tggacggcga cctcgcgctc gcggatgacg
     8161 ctggcgatct cgtccggagg cttgatgttg cggatcagca ccgacttgat cgccacgccc
     8221 cagtccgcgc acttgtcgcg caggtgggcc tcgaggttgt cctggaactg ctggcgcgtc
     8281 tcgccgacga tgaagttgat ggccgggtgc ttgctgccct caatacggct gaaaccgcgg
     8341 gcgcgcggca ggatgacctt cttcaggatg tcgtccatgt cgccgacgcg atgcgtgatc
     8401 agcgcggcct tctcccggat cacggcatat tccagcgtgc cctcgacgtt caccgtgaag
     8461 ccgtccacgg tcaggaaggt gatcgcatcg tccccgctca tctcgaaccg ctggctctgg
     8521 aggttcacct ccgagacggc gaagaggtag gggttgaggt aatacgtccc gggctcgagc
     8581 acctcgggca ccacgccctt gagccccgcg ccgacgagga acgtgttgcg ctcctcgggc
     8641 ttcagttcgc tgttcagcac gtcgcgcccg gcgagcgagg tcacgacgcc gaccgagccg
     8701 ggccggatgg aaatggcgtc gaagtgctcc acgtcgtagg cgtacgggtt gatgcggtat
     8761 ttcccggggc gcaggacgtc ggccaggatg cccttggtgc cctcgccggc gacgatctcg
     8821 ccgggcggca ggtcggcccc gtacaggcgg gtcaggacgc cgagcttgcc cgccgggatg
     8881 tcggtcatcg ccgcgatctt ccagccccac gagtaggggt tgcggaagta gcgcccctcc
     8941 gggaggacct cgagctggat gcccttctgg cccggctcca gcgcgaggat ctggcccgag
     9001 ggcaggtccg cgccggtctt gtggatcagg atcgcgatct cgccggctgc cggctcgatc
     9061 cgccagaaga accagatcca gagcggcagc gcgaggacca gggcgatcac gagcagcgcg
     9121 ccgccgcccg cgaacacggg cagcgcggcg ccccgcggct tggggcggcc gtgaacggga
     9181 accgggaacg aatcatcgct catggtatcc tccgtgatag agcgtacggc gggcagtttc
     9241 ccacagcggc ttcggaggat gaaagctttt tcgggccggc gccgcgggcc tacggcaggg
     9301 cgcgggcctt gacccggaac gcggcccccg cgtcggcggg cgtcgtggtg aattccaact
     9361 gcgccggcgc atcgcctttc tggacagccg cgtcggcgtc ttcagtccat gtcttcagat
     9421 cctcggactg ctcgacgacg gcgacgacgg gccgcggcga atcgagctgg atcccgccga
     9481 gcagcgttcc gccggccagg ttcaggctgt cgatctcgtt gccgacgccg gcgtacaggt
     9541 cgtcgtcatc catggccagg ccgaaaaaag tgatcgccgt gctggaaagg ttggagatgg
     9601 cccatccccc catcaggcgg atgggggccg gtgttgccgt gagttgtccc ggggaggggt
     9661 tcgtgatgta catgtagtat gcgccgctga tgctgtcgat gccggaaccg gccgtgttga
     9721 ttttaaacag aatcgaaacg gtcctcgatg tgaattgcat gtggtcgaag accaccactt
     9781 ttccggctgg aacggtcagc gtgctgaagg cggcaacatc cccggccgga tacagcctct
     9841 ttgcatacgg caccacggcg gcccaggttc cgaaccccat cagaaacacc atgaaacacg
     9901 ccacgctcct gatccttttc tgcatgacgc cctccttgtg gttcgaacag ctgtccctgt
     9961 gtgtattttg gaccccggcg ctccaaaagg caaggacaag caggggcgcc gttgacggcc
    10021 cgccggggcc gcgtataatg ccgcgccatt tcccggagac ccgacgatga aacgacgccc
    10081 gcccgcctcg aagccgaagg tccgtgtgcc cgactgggcc gccgacgcga tctggtacca
    10141 ggtcttcccc gagcgcttcc gcagcggctg cccggccagc gacccccggc ccgaggacat
    10201 ggacgcgagg ccggttcccg gctggaaggt ctcgccgtgg ggcatggact ggtacgcgca
    10261 ggcggactgg gagaaggcgc gcggcgagtt ctaccgctcc gtgttccacc gccggttcgg
    10321 cggcgacctc gtcgggctcc gcgagaaact ggactacctc caggacctcg gcgtcaacgc
    10381 gatctacctg aatcccatct tcatggcgcc gtccctgcac aagtacgacg ccgcctgcct
    10441 ccaccacgtc gacccgacct tcgggcccga ccgcgaaggc gacctggcgt tgatcgcggc
    10501 ggcgaacgag acggaagatc caaggacgtg gatctggacc gcggcggacc gctatctcgc
    10561 agacctcgtc gcggacgtgc accggcgcgg catgcggatc atcctcgacg gcgtcttcaa
    10621 ccacgtcggc acgcacttct tcggattccg cgacctggtg aagaacaagc ggaactcccg
    10681 gtaccgggac tggttccgca tcaaggaatg gcgcgccgac ggcacgttcg actaccaggg
    10741 ctggttcaac cacgccgggc tgcccgagtt ggcgcggagc aagaccacgc tggccaagcc
    10801 cgtgcgcgac tacatcttcg ccatcacgcg ccgctggatg gacccggacg gcgacggcga
    10861 cccggccgac ggcgtggacg gctggcggct ggacgtcgcc ttctgtgtgc cgcacggctt
    10921 ctggaaaagc tgggcgcggc acgtgaagaa gatcaacccc caggcctata cgaccggcga
    10981 ggtcgtcggc tacgcggacg agttcctgcg gggcgacgaa ttcgacgccg tgatgaacta
    11041 catgtggctc tacccgacgc tcgggttctt ctcgccctcg ccggacgcgc tgccggcctc
    11101 cgaactgcgc gcgaagctgg acgagttgtg gcgccggtat ccgcgggcgg tgaactacgc
    11161 gctccagaac ctgctggact cgcacgacac cggccgcgtc ctgacgttcc tggagaacgc
    11221 ctgcccgccg ttccgcgact gggacagcta cttcaattat cccaaggccc gcgagaaccc
    11281 ggggctgaag accacgcggc ccgggccgaa ggcgatggac gccctccggc agatgatcgt
    11341 gttccagatg acctgcctcg gcgcgccgat gctctactac gggaccgagg tcggcatgtg
    11401 gggcgccaac gaccccgaca accggcagcc catgctctgg gacgacatcg agcacgcgcc
    11461 ggaaacgcgc acgctgaccg gccggtgtcc gtcgaacccg agaaagcccg accgggacct
    11521 gttcgccttt actcgccggg cgatcgccct gcggaaggag cgccacgtgc tgcggcgcgg
    11581 ccggttgcgt tggttggaca ccggcagcga acggttgcta gccttcgagc gtttcgaccg
    11641 gaagacctcg attcgcgtcg tgctcaacgc gggcgacgcg ccggcggaaa tccggctgga
    11701 gaaaccggcg cgcgacctgt ggaccgggaa aacggcgcgg cggggacgct tggccctgcc
    11761 gccgcgaggc tgggccgtgc tggaaatcca aggctagggc gacgcctccg gcggagccgt
    11821 cacgcccccg gcccgtcagg gcaggcgcca gtcacgatcc ggtagtcgga catctttttt
    11881 ttagcgcgct caaccatcac gccgaaggaa ttcgactgca ggcggccagt ccaggtctcc
    11941 gcggtcttcg ggttcgtgtt gcgggcgccg tagagcctcg cccggaactc ggtgatgccc
    12001 gggcgatcaa agtagtaggt gcggatttcg acgcgctggg cgatctgctg gcccggctcc
    12061 aggcacacca gttcgctgcg ctcgaggtgt tcttcctcgc gaatttcctt gaggaaatta
    12121 tcgtgcacgc cgttcgggta gatccagctc accagcaccg tcggctggcg gggcagccac
    12181 acgcgctctt cgctcacgtt gcggatggcg acgtccaggt acagcggctc gccgatggag
    12241 atcaccgggg tgtccgcctt cagcgcgatc tcgagcgcgg ggtcttgctc ggcgttatcc
    12301 agccgcccct gcgtggcggg cttgtagcgc tccggcatgc tcgcgcaccc tgacccaaaa
    12361 aggccggtcg tcacaatgac tgccgctatc gttcgcgcgc gcataaaacc cataaaataa
    12421 tatgcagctt tcgtaccagc gatcaattcc gatgtgtaat tgatttgtaa tccgtttatc
    12481 ataaatggga taagatttgc ggtttcgatc cgggacggcc cggtgttgcc gcctattcta
    12541 aaaaatgata gcgatgatcg gcaggcaaaa cgggagcgct ccatgaagac gtggatgatt
    12601 tatttcacgg ccgggacggc ggcggaggcg gggaagatcg ggcgcgcgct ggtgcgggat
    12661 cgcctggcgg cgtgcgtgaa catcctcgga cgtatccgct cgatctaccg gtggaagggc
    12721 cgggtggaag acgggcgcga agtggcggcg ctggccaaga cctcggagcc gcgcctgccc
    12781 gccgcgatcg ccgcgatccg gaaacgccac agttacgaag tgccctgcat cgtcgcctgg
    12841 ccgttggagc gggggcatgc gccgttcatg aaatggatcg cggatgagac ggcggtccga
    12901 acggacagcc gatagtgccg cgtggtcgcg tggacgaacg gttgaacaca ggaaggtggc
    12961 gcgggcgttc cgcgtacggg cggccatcgc gacggagcgc gacggccacc cgatagattc
    13021 cgccggggaa gagtccgatc accgccagaa ccggcggaag ggcgcggagg ccaggaccga
    13081 ggtggagcgc gcgtcggcgg tgaacacgcc cttgacgcga taccacatcg gagggttctc
    13141 ctccgtggtc agcgccagcg cccacggctt gtcgggcggc ggcgggacca tgaacgtcgc
    13201 gggctccgtc ccggcgttga agatcatgaa gaggctgtcg tcgtcctccg gcccgccggt
    13261 atgctccgac cggccgttga gcacgcaggc gatggcgcag tccttttccc agtccggggc
    13321 gcggccgttg atgccgtgcc acgtgatgtc cgcgttggtc gcctcgggct tcttcccgtt
    13381 caggaagcgg gtgcggcgca gggacgggtg cgccttgcgg aaggcgatga gttttttcac
    13441 gaaggcatgc aggtcccggt tcgcctcgag caggctccaa tccacccagc tgatctcgtt
    13501 gtcctggcag taggcgttgt tgttgccctg ctgcgtccgc gaaaattcgt cgccggcgag
    13561 caccatgggc acgcccatgg acaggaagag ggtggtcagg aggttcttct gctgccgccg
    13621 gcggacggcg aggatgtcgg ggtcgtcggt cggaccctcg acgccgtagc cgctgccgtg
    13681 gttgtggttc tcgccgtcgc ggttcttctc gccgttggcc tcgttgtgct tgccgtcgta
    13741 cgagaccagg tcgcggagcg tgaagccgtc gtggcaggtg atgaagttga tgctcttcat
    13801 cggggtctgg ccgagcttgt cgtagaggtc cgcgctgccg gtcaggcgca tggcgagctg
    13861 gccgagcatc cccgggtcgc ccttccagta gcgccggatc tcgtcgcgat acttgccgtt
    13921 ccactcggac cagcgctcgt tggggaaggt gccgacctgg taggcgccgg cggcgtccca
    13981 ggcctcggcg atgatcttcg tgtcgcgcag ggcggggtcc tcggcgatgt gctcgacgat
    14041 gggcgggtcg ggcagcagct cgccgtccgc gccgcgcgtg aggatgctgg cgaggtcgaa
    14101 ccggaagccg tccacgtgca tgtggaggac ccagtaccgc aggcagttca tgatgaagtc
    14161 gcggacgacg gggtggttgc tgttgaccgt gttgccgcag ccggtgaagt tccggtagtg
    14221 ggggcggccc tcctccatga ggtagaagat cgcgttgtcg atcccgcgga acgagtaggt
    14281 cggcccgccg tcgccgttct cggcggtgtg gttgaagacg acgtcgagga tgacctcgat
    14341 gccggcctgg tgcagggcca tgacgagctg cttgaactcg cggacctgcc ggccgtagac
    14401 gccgtcgcgg gcgtagcgtc cgttcggggc gaagaacgcg agcgtgctgt agccccagaa
    14461 gttgcgcagg tcgcggcgcg ggtcgtcggc ctggtagtac tccatctcgt tgaattcctg
    14521 gatcggcagc agctcgacgg ccgtgacgcc caggtcgcgc aggtggggga tcttctcgat
    14581 cagcccgcgg tacgtgccgg gcgccccgac gcccgaggac ggatgcgccg tgtagccgcg
    14641 caggtgcatc tcgtacatga ccgtgtcggc cagcgggatg cgcagcgtcc ggtcctcggt
    14701 ccagtcgaag tcgtcgcgga cgatcacgcc cttggggaac agcggcccgt ccttcggcgt
    14761 ctcgcccgcc ttcaggccgc gcgcgtcgcc ccacttcggg gcgcccgcga ccgccagggc
    14821 atacgggtcc agcagccact ggtccgggtc gtagaagttg gccacgcccc gcggcgtccg
    14881 cccgtccatg cggtagagat agaactggcc ttcgcgcgcg tcctcgacgt gcaggtgcca
    14941 gatgtcgccg atgcggttgc ggtccgggga cagctcgagc tcctcggcgg gctcggcgtc
    15001 gtccgggttg tcgaagagca tcagccacac gcgcgaggcg tgccggctga acagggtgaa
    15061 ctggacgcct ttttccacga cgtgcgcgcc gtagggcacc agcagcgggg tgctcggcag
    15121 gaggtgctgg acggtttgtt ttttcatgga tcgatggcgc cgggacgggg gtggcgcgca
    15181 gcgccgaacg tccgacgctc agcctccaac gtccagcgtc caagggttgg atgccggttg
    15241 ttggacgttg gctgttggac gttgaaaccc gatttcaccg cggtccccgg aagaggtagt
    15301 acaggcgctg gccgaaggtg cgcaggaccc ggcccgcgcc ggtgctggcg tgccggaccc
    15361 ggcgccgcgt cacgcggacg gcggtctggc ccgcctggac ggcgccgcgc gtcaggtagg
    15421 tgccggcctg cacgacgccg gcgccgacgg cgacgacggg ctgcaccacc agcgtgtcgg
    15481 ccatgcgggc cgtcaggtcc acggccgagc cgatgccgtg ggcgatcgag tcccagaacc
    15541 cgggctggtc cgccgcgccg ggctgggccc cggagcggat caggcccttc aactgcggca
    15601 ggagatcctt cgtgaacagg tcccggacgt ccgccaggaa gccggccatg cgctcgccga
    15661 gcgaccggcg ctcctcctcg gcgttccagc cgcggaacga catgcagcgg tccatggcgg
    15721 cgtggccggt gatcgaggtc atcagtccgg cgccgaggcc ctgcccgatg ccttccacga
    15781 cgcggccgac cagcgggacc tcggacagca ggctggcgaa cagcttgcgg gtcaggccca
    15841 gcgcgttcac ggtggccacg gtgaccagga catcccggag gatgcgccac gagtcgccgg
    15901 gcgcggggcg gccgtggtag aggcggacga tgcggccggc catcgccagg ttgcggtagg
    15961 acaccatgat caggtcgagg gcctggtagg ggctcaaggt cacgcccatc atcacgtcgc
    16021 ggacgctgtg ccgcacctcg gcggaggcca gggcgttgag ttcggccagg gccgggagca
    16081 ccacctgctc ctcggtcttg aggatcagcc gggcgaggtc ctcgttgagc gggtgggcgc
    16141 ccagcacctc gcggatttcg ccggcgccct gccggagaac cgattggcgg tcggcgccga
    16201 gattcgggtt gcgggccatg tgattcaggt aatccacaag gtatcggcag tatttccgca
    16261 tgcgcacgtg gtccggttcc cgcccggtga cgcggggggg ctgggggatg ggcgggtggc
    16321 ggcggagcag ataaaggatg tacaccagca ccgagaccag gatcaggacg acgatccagg
    16381 agtagatatc ggcaaacagg ggtcgaatac ggtagagcag gatgtaaaac cgcagcaatt
    16441 ccccggcgaa aagcgccacc aggatggccg ccaggatgac ggccagccgg accatgagct
    16501 tcaatacggt gcttatcatg gcagaaccga aggcggagac taggccgaga ccggccgcca
    16561 ctcaagccca ttcacaagac cgcttctgcg cactcgttat ttcttgacat ttgcatggcg
    16621 cagcgtaatt gccgttgacg cctgcgcggg gcgggttaca gttacggctt attcatagaa
    16681 gggggagggg cgcacaatcc cggtctgaca tcgggccccc ggatttcgga ctccgcgaaa
    16741 taatcacatc taattaatga ttaaaaggtt gcgctggtgg acgagctgaa aatacacttc
    16801 gataacgcgc gggaagcgca ggatgtcacc ggcaagcggc tcgagtacct ggcccgcatt
    16861 gaggacgccc tgggggttcg gctcacggcg cgcgacacgt ggctgctggc ggagggggat
    16921 cccgcgaagg ccgccgccgc gctgcgtttt gtggacatcc tgcgccgggc gcgcagcgcc
    16981 ggcatcagcc tgcgcgagca cacggtgatg tttgccctgc gggctttccg cgaaggccgg
    17041 gagcaggccc tgacccagtt gcatgcctgc cggatcgacg tgagccccgg caaggccccg
    17101 gtctttccgc gcaccttcgg ccagcacgcc tacgtggaag cgatccgcgg gacggacatc
    17161 tcgttcggca tcgggccggc gggcacgggc aagacctacc tggccatggc gatggcggtg
    17221 tccaccctgc tgcgcaacga ggtcagccgc atcatcctga cccggcccgc cgtggaggcc
    17281 ggcgaggcgc tgggcttcct gcccggcgat atgcaccaga aggtgctgcc ctacctgcgc
    17341 ccgctctacg atgccctcta cgacatgatg acgccggacg acatcgagcg gcacatggtg
    17401 cgcgggatca tcgaggtcgc gccgctggcc tacatgcgcg gccgcacgct gaaccacgcc
    17461 ttcgtcatcc tggacgaggc ccagaacacg acgccggagc agatgctgat gttcctgacg
    17521 cgcctcggct tcgactcgaa gtgcgtgatc accggcgacc tgacccaggt ggacctgccg
    17581 ccctacaaga cctcgggcct gctcgaggcc cggcgcacac tgcgcgccgt ggagggcatt
    17641 tcgatcatcg agctgtcgga cgcggacgtg gtccgccacg agctggtcca gaagatcatc
    17701 caggcctacc gcgagtcccg cgaggcggcg gccccgccgg cggaacagga gtccgccaag
    17761 taatgatcct cccctggagc agaggccggg cgttccggcg cgccccgctc ctggccgcgc
    17821 gcaagacggc gcgcgcgccg gtgccgaccc tgccgctgct cctcggcctc ctcctgtggg
    17881 tctccgctct ggtggtcctg aacctcacgc ccgaccggcc ctacagcggg cttaccccgg
    17941 gccagcgcgc ccccgtcacc gtggtggccc aggtggattt caaatgcgag gacctcgcgc
    18001 ggaccgagat gaaccggcag caggcggcga acgccgtccc gcccgccttc accatcgacg
    18061 cgaccccgtt gaacaccgcc gcccgcgcgc tggacaagct cgtcgaccgg ctcatggccg
    18121 agcgggagcg ccaggccggc ctgccggctc cggatcccga gggcctggtg cgcgccctgg
    18181 gcaacgcgct cgacctgctg ggccttccgc tgacgcccga cgaagtacag gccctcttcc
    18241 ccgccggcgc ggaggccggc gcgcgggacg ccatcaagaa ggccgcgcgc gaggtctggg
    18301 ggcacggcat catctcgccc gatgaaaagg cgtccgcttt ccagggcctg gcgtcaaccg
    18361 gggccatcgg cgtgcaggcg gcggacggcg cgtggcgcag cccgctgccg ctggacgagt
    18421 tgccgctgcc cgccgccgcg gtggacgcca tggtcaagcg catcccggag aaggtcggcg
    18481 agaacgcgct cccgcgggag gccctccggg cgctgctgaa gccgtgggtc ggcccgaacc
    18541 tggcctacga tccccgcacc accgccgagc agcgccgggc ggccggcgtg gccgtcgagc
    18601 cggcgatcgt ccgcgtgacg gccggcacga tgctggtgga ggcgggcgag ccggtgaccc
    18661 cgcaggtgct ggagtacctg cagaagcacg agcgccggcg ccggcagctg gagtcgccgt
    18721 acgacctgct gctgaagcag atcggcaacg cgggcctgct cctgggcgcg ctgatcgcgg
    18781 gcgcgggcct gctgcacgtc ctgtcccccg cgctgctgca gaacccgcgg ctcctcctgc
    18841 tgctgatcgt gatctcgctg gtctcgctgg gcccgacgcg cttcgcggtg aacgtggccg
    18901 gccgcaccgg gttgctctcg cccgcgctga tcgagttcgt gctgcccctc gccctgtcgc
    18961 cgctgctggc ggtgatcctg ctcggcgtgc cggcggccct catggtgggc gtgtggaaca
    19021 gcctggtgat ggcgatcctg ttcgaccaga gcttcagcat gctgctcctg ggcctgttcg
    19081 tcaccgccgt ggccacgctc tcggcgcggg acgtgcgcca gcgctcccgg atctaccggg
    19141 ccggcgtcct gatcgggctg gccgaggtcc tggcggtgat cgcgttcgcc gccgtgcgcg
    19201 gcctgccgcg gacggcgctg ctgatccagg ccgggacggg cctggcgggc ggcatggtct
    19261 acgccctgct ggccatcctg ctgatccctc tgctggaatg ggcgttccgc gtgacgacca
    19321 acctgacgct cctcgaactc tgcgacttga gccaccccct gctgcagcgc ctcgcgatgg
    19381 aggcgccggg cacgtatcac cacagcatag tcgtcgccaa tctcgcccag gaggcggcgc
    19441 agaagatcgg ggccaacacg ctgctcgtcc gcgccgcggc gtacttccac gacatcggca
    19501 agctggccaa gcccgagttc ttcatcgaga acatgcgccc cggcgtgaac ccgcacgaca
    19561 ccctggcgcc gcgcatgagc agcctgatca tcacctccca cgtcaaggag gggctcggcc
    19621 tcgcccgccg ctaccgcatc cccgccagcg tcgccgccgg cgtcgagcag caccacggca
    19681 acgggctggt gtcctatttc taccaccgcg cccgccagca gaaggaggag ggcgaggcga
    19741 acgagtcggc ggtccccgac caggaatacc gctacggcgg ccgccgcccg cagacccgcg
    19801 agatggcgat cctggccctc gcggattccg tcgaggccgc cgcgcgggcg ctggaggatc
    19861 ccacgcccgc ccgcatcgag aacctggtcc atgacatcgc cgccggaaag ctccaggacg
    19921 gccagttcga cgaatgcccg ctgaccctcg gggaactggc cacgatccgg cgctcattcg
    19981 tcttcagcct ggccaacatg ctgcacgtgc gcattgccta tcccccggat gaaactaagc
    20041 ctccacaacc ggcagagccg gatctctctg aagacggcga acctgaagaa agcgacccgg
    20101 tggctggcgg accggatcgc ccgtgattcg ggcctcgccc cctggagcga actggcggtc
    20161 accttcacgg acgacgaggg catcgtcccc gtgaatgccg cctgcttcgg gaaggcctgg
    20221 cccaccgacg tcatcaccca gccctacatc tcgccgccgg gcggcgacgt gatcgtcaac
    20281 gtcgagcgtg ccctggccgt cggcggggcc ggcccccggg gcgaggccga actcgcgctc
    20341 tacatcgccc acggcctcca ccatctggcc ggcgccgtgg acgacacccc cgcccgccgc
    20401 cgcgccatgc tccggcagga gcaggcctgg ctgcgcgcag cggaaaacga gggcttgctg
    20461 gcggctctct ggagggcggc cggccccggc cgccgctgaa ttaccccagg tccacccccg
    20521 gcaccttggc ctggcaggcg gggcagcggc cgccgcggat gtcgttgcgc aggaccttga
    20581 acccgacccg ctcgatcagc gtccgccggc aggcgggaca gaccgtcgag gcgccctcgt
    20641 ggccgggcag gttggcgaga tagacaaatc gcaggccttc tttcatggcg atatcccgcg
    20701 cctgctccag cgtctcgcgc ggcgtcggcg gcaggtgctt gagcttgtac gcgggcgcga
    20761 agcgcaggaa gtgcagcggg atgtccgggt tcagccccgc aagcgaccgc gcgatggcgc
    20821 ggatcccctc cggctggtcg ttcatcgtcg gcacgatgag gttgaccacc tcgatccagc
    20881 ggccggactg cgcgatggag cggatggtct gccagacgct gtcccgcggg cacgcgacga
    20941 cgtcccggta gaactgctcg tcggcgccct tcagggtcac gctgaacgcc gcgccgcatt
    21001 ccagcagggc cgccagcggc ttcgggtgga tgaatccacc cgtgaccacc gcgcagcgca
    21061 ggccgcgctg tttcgcgagg cgcgcgacgt ccagggcata ctcgaggtag gcgacgggct
    21121 cggtgtagga aaaggtgatc cacttcaggc cgcgggagac ggcgcgctcg acgagcgcgt
    21181 ccggcgccag gctcatgttg cgcgtctccc acgggccgta ctgggagaac tcccagttct
    21241 ggcagtacag gcagcgcagg ttgcagccgg ccgtcccgat gccgagcgcc tcctggccgg
    21301 gggtgacgtg gtagagcggg ttcttttcca gcgcgtcctg gaacacgacg cagggccggc
    21361 cgtacgtcat ggagtagagc cgcccgccca ggttgattcg cgtgcggcag gcggtgacgc
    21421 cgccttcggc gcacacgcac tcgttggggc aggtcaggca ccgggtgcgc ccgtcgccgg
    21481 ccggctccca gaaggccgcc tcgcgcaggt cgcctgccgc ggcccagcgc gcggcgcgcc
    21541 ggtcctgggc gaacgcgggg caggggatcg cggcgccgag ggccgcgccc agtccgccgc
    21601 acgcgcagcg gcccaggaac tggcgtcgtg tataccgggc ggtcatcggg tcatctcgcc
    21661 agcttggaaa gcagttccac cccggcgtcg gtgaagagca gttcggcgcc gggccccagg
    21721 accggcaacg gttcgccccg gtcgtacttg gtcccgtagg cggtttcccg gggagccgag
    21781 cggcgcgggc tcatctcggc caccaggtcg cgcaattgcc gccggaacgc cccctggctc
    21841 atcccgcggg ccttttcgag gacgtcctgc gcgcgctcgc tttcggtggg gatatccacg
    21901 cccatgtccg gttcctggcc gacgcattcc cggacgaagc ggtcggccag gtccgcgccg
    21961 tcgcgctgga actgcgcggg ggacatggcc cgggcctctt cgagggtccg cagggccagg
    22021 tggtgctggc ggcgggcggg catcgggccg ccctggtcgg gcgacggcgg gttcggggcc
    22081 gcggcgaaca tcgccgactg gttggccgtc aggacctgct ccgcctgctc ctcgtacggg
    22141 cgcagcgcgg cgaacaggtc ctgctcgtag atcgtcttca ccgcctggtg ccagcgctgg
    22201 caggcctgct cggtctcccg cgtggggttg ccgtccgcca gttcggcgcg gagttggcgg
    22261 taatcggtca gggccttggc cttcacttcc tcggcgcggg catgcacctc ggcgcgggcg
    22321 tcgcgggcgg accgggcgac gggcagcagg cgatcggcct gtccctccgt caggtccagc
    22381 ccggccagca gccggacggc cttcagccgc tgggcgcttc ccagcgtatc ggcgggccgt
    22441 ggcgccgagg cctcgcgcgg cgagacggcc gggaccggcg tgttcgcgcg ctttcgcacg
    22501 acatccaggc agccgggatt aagcacgtac aggccgatca gcttgagctg ttcgtccgtt
    22561 gaagccgggc gctcctcgag ggggatgagg cggcacagga tatcctcctt ttccagttcg
    22621 aggtccacgt cgctcaacga ccggatgcgc ggcagctcct ggtcgagggc ggtttcgatt
    22681 tccttccgga cgccggcctc cgcctcctcg ctgtatttca cgcggcgctt ctggagcaca
    22741 tagcgcgtca gggcctcggt cgcgcggtcg cgggcttcgt cgagccggaa ttccggaact
    22801 tcgcgaaggc gcttcaggat cggctccccg acgccgagat gattttcggc ctgcccgacg
    22861 cgcaccgggt cgcggaagtc gccggggggg atgaaacagg gcgtgaagcg gtcgatgatc
    22921 tcgacctggg cgggcgtcag caggccatag acctcccgcg ccaggccgcg gacctgtcgc
    22981 tcccgttctt cgcgctgggc ctgaatatcc ttgagttcgg gcggcggtcg cgccatgcgg
    23041 ccctcgccgc cgttgcggcc cgccaggcgg gcgagggcgt ctgcgtcgcc gtatcgccgg
    23101 accagcgact cgacccgggc ggcggcctcc gtttccatcc gctccatctc ggacagccgg
    23161 gcggccaggc cggcgttctg tcccggcgtc aggaacaaac cgttcagcag gtagagcgaa
    23221 ggaatgtcgc gctcgagttg gagaaagtcc gagccgtttc cggcccgcgc cgtggcgccg
    23281 acgagggcga gggcgcccgc gagcaatcgg accgccgctt gcatcttcat ggccaagcct
    23341 ccccttctga tactcctagt acgttctcct cgtccattct attgtgccga gtcgcccgcc
    23401 gggaagaaaa ccacaacaag cccgcgtccc ctgttcatgt attatgctca cacttctaga
    23461 agtgtgagca tggcggtcat ggtcatatca gattgcacac aagaataata cgtataattg
    23521 ccatgcgaga cttgttaatg gttctgctca cacttctaaa agtgtgatca tggattccag
    23581 tatcgatggt gcggcggtgc gagagggtcg ggcggcttga tcccgccgag ggatgggcga
    23641 cacacatgcg cggttgtcgg gaggcgcctt tcctcttgct ccttggtcga agccctgtaa
    23701 ggtatccggc agcatcggga tgcaaacatg tttcgtaaaa tcctcaaggc gctgcgcaac
    23761 aatatcctga ccggcctgtt cctggtcacg cccatcgcgg tggcggtctt cgtggtgctg
    23821 tggctcttca ccctcatcac cgaccggatc atgccctttg tcccgcggaa ggtgggcgag
    23881 gtctacccgg aaatcctgtt ccgcctgatt gccttgctga ttttcctggc ggtgctcttc
    23941 ctcctcggcc tgctgatccg gaatatgatc ggcgctcggt tgtaccgcat cggagacgcc
    24001 atcctttccc gcattcccgt gttcaacaag atctacatct ccgtgcggca ggtcggcgag
    24061 gccctgctgg cccagagcca gaccctcttc caggaggtcg tgctcgtgga atacccgcga
    24121 aaaggcctct tttccatggg cttcgtaacc accccggcgc cgccggaatt catccgcaac
    24181 ctgcccgacg gcgtcgccac gcccctcgtc gccgtgttca tcccgaccac gccgaacccg
    24241 acctccgggc tgttcatctt cgtgccgaag agcgaaacct acaagatgcc catcggcgtc
    24301 ggggacgcga tgaagctcgt ggtctccggc ggggcggtgt acccgggcgc tcccggccgc
    24361 gcggacagcc ggcctacgtt cttggacaag ctccagaact gggcggagcg cgagggccgc
    24421 gatacggcgc ccggcgccgg gccctcatga tcctcaaaac gacagccctc gcgctgcgtc
    24481 tctacccctt ctccaacacg tcccggatcg tgtcgtggct gaccccggac cagggccgga
    24541 tcgtcaccat gatcaagggc agccagcgcg cgcgcagcct gttcctgggc cagtacgacc
    24601 tgttctacac ctgtgaactg ctgtatcacc gacgcgagcg cggcggcctg cacatcgcgc
    24661 gcgaatgctc gccgctgaaa tggcgcgcgg gattgcggca tgactggaag gccgccgcca
    24721 cggcatcgta tctcgccggc ctcgttgccc aggcggttcc gccagaggcc ccgcatgaaa
    24781 gcctgttccg gttgctggat tccgcgctcg accacctgga agccgccggt gcgggggagc
    24841 ccttcgtatt ctggttcgag ctgcgcctgc tggaggcgct cggactgtcg ccgcgcctgc
    24901 ggaactgcgc cgcttgcgga gcggaactgg cgtcatcctc ccgccgctcg gatttcgcag
    24961 cggctcgcgg aggcttgctc tgtgcgggct gcgtggacaa gacgtcttcc ggcccgcgtg
    25021 tcgggggcga cgtgctcggc atgctcgcgg gctggcagca ggcccgtttg ccgcaggggc
    25081 cgctgaacac gaaatgctcc gcgcgccaga tccaagccat cgaggccctg ctcagcgggt
    25141 tcctggaata ccacctggat gtccggcccg tcggccggcg cgaggcgctc gatgtcctgg
    25201 cccgacgcgt ggcgtgagtc gcgcggccgg ctattttccc cgcagcagat cccagagcga
    25261 cccgagcgaa acgggcgtca cctgggtttt cccgagcacg ggcatgaagt tcgtgtcgcc
    25321 gttccatcgc ggcacgatgt gcaggtggac gtggtccttc agccccgcgc ccgccgcctc
    25381 gccgatgttc agccccacgt tgaatccgtg cgggtgaagc gccttccgca gcaggcccaa
    25441 cgatcgcatc atcaggcgta tcatctcgtt gagttcgggc ttggtcaggc cgtcaagcgc
    25501 cgcgacgtgc cggagcggag cgaccatcag gtgtccgccg gtgtacgggt agcggttcag
    25561 gaccacggcg caggtccggc cgcggaggac cacgccgttc gcccggtcgg cgcgggagcg
    25621 caggctgttg cacaggaagc agccgctctc gcgcggccgc ttgatgtatt ccatgcgcca
    25681 gggcgcccag aggatatgac gcggaccttt tttcttggcc accatgcacc gccttgcttg
    25741 aaaacgaggc gagcataatc cggcggcggg gagggtcaag gcgcgattcc cgcgggaaca
    25801 gggctcgacg gccggacctg cggggtgcta tgctgccgac gctttgggaa atcgccgctc
    25861 gagtccgggt catggccaaa gagcccgaaa agaaagtgct ggccggccgc tatgaaatcc
    25921 gccgccccct gggcgccggc ggggtcggga tggtctacca ggcctgggac ctccaactgc
    25981 accgcttcgt ggcggtgaag cgctggcggc cgccggacga cctggtcgag gacagcgccg
    26041 gcaccgagaa gctctggcgc gaggcgatga cgctggccgc catccagcac cccaacatcc
    26101 tgaccatcca tgacttcggc gtggaccccg aagggccgta cgtgatcatg gagtacgtgg
    26161 acggcgagac gctggaccgc gtgatccagc gcgggccgtt cgaccgcgac tctttcgccg
    26221 aggcttccca gcagatctta gaggcgctga tcgccgcgca ccaggccggg atgatccacc
    26281 gcgacctcaa gccgcagaac atcatgcgga cgcggctgcc cagcggcggc tggcagtaca
    26341 agatcctgga tttcgggctc gcgcggttcg tcacccagcc gaccgtccag agcatggagg
    26401 gcaacacctc gatctacggc tccatcttct tcatcgcgcc cgagcagctc aaacaccaac
    26461 cgctcgacgc gcgcacggac atctacgcca tcggatgcgt ctgctactac atgctcgccg
    26521 gccgctatcc cttcgagggc gattccatcc ccggcgtgat cacgagccac ctcgagcaca
    26581 acgtcaagcc gctgggcgac tggcggcccg acctgccgcc ctccctgtgc aactgggtga
    26641 tgaaggccct ctcgttctcc ccggacgagc ggtggggcag cgccgccgag gccctgaccg
    26701 ccctgcgccg gatcctgccc ggcacggtgc gcacgacgac catcaagatc gtcacgccag
    26761 tccgcggccc ccagatcacg ccgctgggcg cggagtccgt ccccccgacc acgggtatgc
    26821 tgaagatcag cccggtgaag aagccggagg gcgccgccga atcgccggcg gggcccggct
    26881 tgttgcggcg cgcgctggtc tacatggtgc ccctggcgct gctggccgcc atcgtcacag
    26941 ccctcgtctg gctccgggct cccccggcgc ccccggaggc gattccggcg gaacccgcgg
    27001 ccgactcggc cgccagcacg gaaatcaccg cgtggccccc gtacatcccc gaggagcgcc
    27061 gcgccctggt ccagctctac ttcaagcaac tgcgcgacct gcaaggattc aagtgggcca
    27121 gcatcgggac ggtcatggag gcgctggccg ccgttcagtt taaggagcaa ttcgacccga
    27181 gccagatgca agcgttgccc aatatatcct accatgacga gaccggccgg accgtcggcg
    27241 aactggacgt ggtgctgtgg aacgtgccgt ccaactgcgc cgaggtggtc tacgaggccg
    27301 tcatcaccga caacctgcgc cgcaacaagg cgtccagctg gacgcaattg aaccgtttcc
    27361 gcgtcgccgt tcaggagcgg accgttcacc actttattta tgcccacgac ccgacctgga
    27421 aaatggatat ctcccagttc gaccacgccc ggtacggcct gatgggcagc cgcggcgccc
    27481 tcgcggaggg ttacgaatcc gaggtggaca tcacccgggc cgaagccgaa ttcctccagc
    27541 gccgcatcat cgagtaccag aaaaaccgcc acgccggccc ctgacccgcg cgaatttttc
    27601 ccttccggac agcgcccctc gtttggatat acataaatgt agatatatgt attttatgat
    27661 acaatatagt atgttataac gggcttggtt aattttaggc atttcatgta acacattggt
    27721 tcctggatcg atgcgactgc tttcctccgg tttcccgcgt cgcttggcat gagcgctgct
    27781 aaagcgcgta gcgtcacccg cgcggtgcgg atggcttggc gaaagacgcg gaatgaaagg
    27841 gatgcttatg aaatggaaca ggtgggcggt cggagggctg tctttgctga tgctggccgg
    27901 atgggccggg gtggcccggg cgcaggaagc cgggccggtg ttcacgcagg agatggcgga
    27961 cacggtcgcg aactacaaga tcactctcga taccctgtgg gtgctcatta cggcctgcct
    28021 ggtgttcttc atgaacctgg ggttcgccat ggtggagtcc ggcctgtgcc gggcgaagaa
    28081 cacggtgaac atccttgcga agaacttcat cgtgttcggc atcgcctcgc tggcctatta
    28141 catcttgggc tgggggctga tgttcggcga cggctccggg ttcgccggac tcaagggcct
    28201 gtggttcgcc gcgggggccg acaacagccc ggcggcgggc gacgcctacc agggcgtcta
    28261 ctcggcgatc agctgggcga ccgtcccgtt ctgggcgaag ttcttcttcc agcttgtgtt
    28321 cgccggcaca gcggcgacga tcgtgtcggg cgccgtcgcg gagcggatca agttccactc
    28381 gttcatgctc ttctcgttca tcctcgtcgc catcctgtac ccgatcacgg gccactggat
    28441 ctggggcggg ggctggctgc agaggttggg cttctgggat ttcgcgggct cgacggcggt
    28501 gcactcggtc ggcggctggg ccgcgctcgc gggcgtcctg ctgctcgggc cgcgcctggg
    28561 caagtaccgc ccggacggca agccgcgccc ggtcttcggc cataacatgt ccacgacggc
    28621 gctcggcgtg ttcgtcctct ggttcggctg gttcgggttc aatcccggct ccaccatggc
    28681 cgccgcggcc ggggatatcg cccggatcgc cgtgacaacc aacagcgcgg ccgccgcggc
    28741 cagcttgagc gcgacgatca tggcgtggcg gctgctgggc aagccggacc tgtcgatgat
    28801 cctgaacggc tgcctcgcgg gcctggtcgc cgtcacggcg ccctgcgcgt tcgtgagcgt
    28861 gccgatatcc atcgtgatcg ggctcatcgc cggcgtcctc gtcgtgctcg cggtgctgtt
    28921 cttcgaccgg atgaagatcg acgacccggt gggcgcgctc tcggtgcacc tggtcaacgg
    28981 cgtgttcggg acgctggccg tcggcctgtt cgcgcaggcg gacatcatgc cgaacacgac
    29041 cggcaacggc ctgttcttcg gcggcggctt cgcgctgctg ggacaccagt tcctcggggt
    29101 gctggccgtt ggcggcttca cgtttgccgt ggcgctggcg gtatgggcgg tcatcaaggc
    29161 gaccctcggc ctgcgggtga gcgaggagga ggaactgcgc ggcctggaca tcggcgagca
    29221 tggcatggag gcctacagcg ggttccaggt cttcacgacg caatgatctg cggaatcaaa
    29281 ggagaataac catgaaactg atcatcgcct acatccagcc cgagcggctg aacgcggtca
    29341 agcaggccct ctacgaggcc gaggtgttca agatgtccgt caccaacgcc ctcgggtgcg
    29401 gccagcagaa gggctaccac gagagctacc gcggcgcgga cgtcgaggtg aacctgctca
    29461 agaaggtccg cctggagatc gccgtgaacg acgccttcgt gaagcctacg ctggacgcca
    29521 tcatcaaggg cgcccggacg gggcggatcg gggacggcaa gatcttcgtc atgcccctgg
    29581 aggaatgcta ccgcatccgc acgggggaga ccggctctcc ggccatcggc tgatccttcc
    29641 accccaggcg gcgtcccggg tcctccaccc cgggcgccgc cctcattttt accgggcggc
    29701 gcgttcgatc tcgtcgcgaa gcttccggta tatccccgcg aagcggccgc cggcccggat
    29761 gccgtcgtcg gcgagcgcga gcgcctcggc ggtcttcccg gtcttcatgt aggacacgac
    29821 cagggcctgg ttgcaggcga cgaggtcctc ggcatccatg ctcgggcggg ccgccaggag
    29881 ataggggatc gcctcggccc actcgccgcg cagttggcac aggcggccga cgtagcgctc
    29941 ggtgagcccc gttttcgatg cgccgtgcgc cagcaggaag acgccctgcg cgatcgcccg
    30001 cgcggcggcc gctcgttcgt cctccgacag gccgccggcc agtttctccc ggcaggcgag
    30061 cgtaaagtag acgtactcga gatgccacga ggtgtactcc ggcacctggc gcgcggcgat
    30121 ttcatacagg ggcagcgcct cggcgggccg gccctcgcgc agcagcgcgc gcgcgatcat
    30181 ggccgtgatc gggcgcagcc cgcccgcgtg cggccgggcg gtctgccact cccgagccgc
    30241 cgcgaggacg ccgggcgaca tgcgcgcctc cgcttcgcgg caggccgtgt tgtgccgggc
    30301 gaatagttcg ggattggcgc gcttcatgaa cgggaccccg tacaacaccc gcagcgtgtg
    30361 gttcacgcga tactcgtcgt acgggttggc gcccagccgc cgcgccagtt cggcgtcgtc
    30421 cgggagcccc gccagcgcgc cgggatcgac gcggagggca ccgggcaggg accccatggc
    30481 ttcggccatc gcccgcgccg cgcgggcctg tccgcgcagc gagaggtgca cgtggtcctc
    30541 gacaaggtcc cggcccgtgg cgccggccgg gctttccgtc cggaagagct cggcgatatc
    30601 gcacagcccg gcgccttttt cccgagccgc cgaccggatc gcctcctcgg tttgcgaggt
    30661 cgggcgccag ggcaggggat cccggtctcg cgcctcgagg aaggaccggc gcgcgccgtc
    30721 ccggtcgccg gcggcggcct gctcgcgccc gcgccggtag cgcgcccgcg cctcgttgtc
    30781 cgcgcccagc ggcgccaggt ccgactcgtt ggcggcggtg gtgcatacga tcgccgggac
    30841 gccggccgcc ttcgcggcgt ccagcatggc gcccaggttc gcggcgaggt tccgcgcggc
    30901 ggcctcgcgc aggggcgaat tcgcgtggat ggcggaccgg ccgatcatct cttccatcag
    30961 cgtcacgccc tcgtccggct gccggccccg ccatgtatcc agggcctgga cgagggcgag
    31021 gccgcgcagg gcgcgcagca cccgaagggc ggacggcggg agggtgccgg cggcgttgat
    31081 ggagccggtg ccgtacgcgc cgaaaaattc gttgttgccc gtgtagaaga tcagcaggtc
    31141 cggctcgaac ttcagcgcgt cgcggacgag gtagaccagc gggaaactgg cgacggcggt
    31201 ggtcccgagg ctgatgacct cggccttccg gtccgtccag gcagagttga gtatttcctg
    31261 gaggaacgcg gccatggaga ggttgcgcgg ctggggatag cccttcgcgg cggactcgcc
    31321 gatgatgaaa atgcggacga cgtccgccgg cttcggcatg acgaaggtgg actgctccgc
    31381 gtaacccggc cggtccgggt tggcgaagaa gtagggtttc gacgcggccg gctcgacgag
    31441 gcagagcatg tccccggacg gcaggcggcc gacctcgcgc aggaacgcgg gatagccacc
    31501 gcggccggac agtcggaggc cggcttcaat cgttgcgagg gccaggagcg gaagcagcag
    31561 ggcaagaagt ctaaacagga ttctcttgcc cggggacatc ggcggccggt gtgcggccgt
    31621 cgcttacgcg cgccggatac ggcggaacac gcccagcccg accagcgcgc ccacgcccat
    31681 cagcatcgcc gtcgagggct cggggatggt gtatgtgctc aaccccgttg tgccggagga
    31741 ggtatacggg ttgggccagt ctgaaatagt ctggttggga ttcgacaggg catccaccgc
    31801 cgagtcggtg ccgccaccgc cggacgagta ggcgtcgaag tagaaggtgt cattgcctgt
    31861 gagcccaagc gatgagcgtg taacagtcat ggtcaccgtg ctggtgggcc cgctaacgaa
    31921 actgtatgcc gccaaggccg cggggccgct ccaattgcca cccgtacccc cgcctgcacc
    31981 tacgctgtat gtccaaagct gggctccgcc acccgagttc acccacgacc cgagccaatg
    32041 tgtcatgccg taccccgatc cggcatcgag attaatgggc cgattccatc catttccagt
    32101 tgttgagccc gcatctttca tgttggcgat gccaatcatg aaattgcccc agtcgggatt
    32161 agtcacatct ccgttgacgg caagtgtgaa gatgaggtcc gacgcggtgt gtgagacttc
    32221 catgcttacg atatccatcg tgccgttggc gctgacaaat acttcagaga gtgaatcgtt
    32281 cacaattata gacgcctgta cggatgccgc cgcgaccagt gatgcaagaa acagaacttt
    32341 tttcatggcc ttccccctgc ttgagttgcc gtgatgatcc aataactagc atgaccgccg
    32401 ggccggtttt tgtcaagccg ccgtgaaggc acgtatttct ttgggcggga agccttcccg
    32461 cacgcccggc ttaccaccac cccatggcct cgtcgacctc catggtcagg tcgcggaggc
    32521 ggcgctcgac gatctgcccc ttggcctccc ggcgggtctc cagcatggtg cggatcttgt
    32581 tcagttcgcg ctccaggttc ttgacttcca gttcgcgctc ggccaggcgc aaatcgaaga
    32641 tttcgttgag catttcctcc agtttctgtt tcagggcggt cttctgcgcc tcgtccgggg
    32701 cctggcgcag cgcctcggcg accttccggc attgggcgtc caactgctgg gcgcgcagga
    32761 acccggcggc cagttccggc gcgacccgct tgagttcgtt gtagcggcgg accatgtggc
    32821 cgagcatgcg gatttcgtgc tggaatatct ccggctcatt ttgccggagc cgctggaggt
    32881 tttcgaaaac ctcgggcagg ttggtctcga ggaaggccag cgtttcctgt tccgagggca
    32941 gggggggctc tcccggcggg ggcggttcct gccctcccgc caccggcgac agcaggcaga
    33001 atccggaaat caacaccacg aactgcgcga actttttcat gacgggctcc tttcctggca
    33061 aaccggtctg gccgcgggcc ctttcaaagg cggatacccg cgaagcgaga ctcttattgt
    33121 cgtgttccgt tgtatcgtcg gcgcgccgcg gccggcgatc tcgtcgtcgg ccggaatctc
    33181 gatcttcagg agatcctcgc gcgccgtatc catttgcgat ttaagctgct gcatcctcct
    33241 gtcgaagtcg gcgccgggcc gggcggggcg ggtggcggcg acctgcgaac ggcagaggcc
    33301 ctggagggtt tccaggcgtt ccttcacgtc cgccgcgaag gtctcctcgg gttcgagcgc
    33361 cggggcgggc gccgccgggg ccaggccgcc gacggccgcc cggagttggg acaggcgcac
    33421 cgccaactcg tcgtcggaga cgaggcccgc cggcgggtgc gtcgtatccc gggcgacctg
    33481 gatgcccggc gcgcgccgcc acagccgcca ggcggcgccg ccgaggagca gcagcagggc
    33541 ggcggctgcc gcccgccacg cgggataaag gaacaggacg cgcgcggtct tccggcgccg
    33601 agccgcggtc agggccggcg cgacgagatc gcggcccggt tcgaggaccg gcagccgcgc
    33661 caggcgcgct tcctgctcct ggaattccgc gagcgccttg cgcgcttcgg gatcggagcg
    33721 cagcgcctcc tcgacccgcg cgcgccggtc cggcgtcagt tcgccgccgg cgtacaacag
    33781 cagttcctcg tggctgattt tcatggcgcc tcaccgaacc acttccgcag ccgctgggac
    33841 gcattgtgca tccggccgag cgtcgtgttc aagggggtct tcatgacctc cgcgatctcc
    33901 cggaaccgca gcccggaata ggtccgcaac agcacgacct gcttttccgc ctccggcagg
    33961 gtggccacgg cctgcgcgac ccgcgcggcc gtttcctgct cctccaactg gcggccgggc
    34021 gaagggcccg ggtccacggc ctccggggcg gggacatcgt gctccgtcca ggccgattcg
    34081 ccgatcggca gccgccggcg cttcttcgcc atccgcaggc cctcccggta tgcgatctgg
    34141 aacatccagc tcttgaacgt tcccttcgcc tcgtcaaacc gatgcagctt cgtcgccgcc
    34201 cggatgaagg cctcctgggc ggcgtcctcc gcgtcgtgcc ggttccggag catccggcaa
    34261 aggaaactga atattcgcgc ctggtatctt ctcaacaagt cttccaaggc cgattcgtgg
    34321 ccgccgcgcc acaaccggat caagtcggta tcgagccgat cgtccggccc gctatgtgat
    34381 acctcggcca tggcggtagt attgccggcg ttcgatggag gctgtgcgat agcgtgcatc
    34441 cccgccggcg gagcgtcaag ccggcatcgc cgggaatacg ggcaataggg ccttcgacag
    34501 gagggtagta ccaccagaga ttggaagaag agtacagtga acgaggcgaa atccttcgcc
    34561 ggggagtgga acatggctgt ccgcgcaatg atggtccacg cggcgctgtc tgtcggcgtc
    34621 ttgaaccttg tccccggcgt ggccgccggc gcgacgtatt acgtgcggcc cgacggcggg
    34681 tccgcgcagc agtgcaatgg gctggtggac gcgccgtacc cggggagcgg cgagggccag
    34741 ccctgcgcgt gggatcaccc ctttcgcgcg ctgccgccgg gcggcgcgcc gaacatcgcg
    34801 ggcggcgaca cgctgatcat cgggccgggg agctacaaga tggggtacgg cgcgcccggc
    34861 gcggacgcat gcaacagcgg ttggccctgg gaatgcgcca tgccgcccgt gccgagcggg
    34921 ccggacccgc agcacccgac gcgcatcctc ggcgcgagct gggcgtccgg ctgcacgaac
    34981 aagccggagt tgtggggcgc ggagcgggcg acgttcatct tcaacctgac cggatcgagc
    35041 aacgtggaga tcgggtgcct ggagttgacg gatcacgaag gctgcgtcga gtttcattcg
    35101 ggcggcctcc cgtgcgagcg cgacagtttt ccgtacggcg actggtgcca gcgcggcctg
    35161 tacgcggagg atgcgcggaa cgtgtacctg cacgatctcg atattcacgg gatggcggac
    35221 acgggcatcc aggccgggcg gctggcggac tggacggtcg aggacgtgcg cctggccggc
    35281 aacggatggt ccggctggga cggggatatc gccggggacg acggcaacac cgggaccctg
    35341 ttcttcagcg gcttcgtcgt cgagtggaac ggctgcggcg agacctggcc gggcggccag
    35401 gctacgggtt gctgggcgca gaccgcgggc ggctacggcg acgggttcgg caccggccgg
    35461 acgggcggaa actggatctt cgtggactgc gcgttccggc acaacacgtc cgacggcctg
    35521 gatctgctct acgttcggga accgggctcc tcgattctca tcagccggac gctggcggag
    35581 ggcaatgccg gcaaccagtt gaagacgtcc gggcccgcgc aaatcgaaaa ctgcgtcgtg
    35641 gtggggagtt gcgccttctt cgacggccag ccgttcacct tcaacgtgga taactgccgg
    35701 gcgggcggca acgccgtgtc gctctccctc gcgcccacca atcaagcgtc catcctgaac
    35761 tgcacgatca ccagcgaggg cgattgcctg gtcatcgccg gctgtgccgg cacgaactgc
    35821 aacggctcgg aatccgtgta tctgcgcaac aacgtgtttc ttgggcacac ggatttcttc
    35881 cagccctggg aaaacacctg cctgacgtac gacgagggct tcccggccac gcccttcgac
    35941 ctcgactact cggtgatttc cggcgtgaag aacgatccct gcccggtggg tccccatgac
    36001 ctctgcgcga gcccgcagct ggccggcatg acgctggccg ccttcgacgc ccggttgctg
    36061 tccaacagcc cggcgatgga cgcggggacc aaccggccgg cggtcggcgg cgatttcgac
    36121 ggcgtctcgc gcccgctgga cgggaaccac gacgggacga accggcacga catcggcgct
    36181 ctcgagtatg cgcacgcgct ggccgattcc gacggagacg gcatggccga cgcggacgag
    36241 gtgacggcgg gcacggacgc cacgaacagc gtggccctct tcacgatgga gatcctccgt
    36301 gcggtgccgg atcccgtgct gggatggtcg ggcgttgccg ggcgggccta cgacctgttc
    36361 tccgccccgg cggtgacagg ggcctgggtg gccgtcgaag gcgggacgaa tcttccgggt
    36421 cgggacgctc cgcaatggtt taccaatgcg gcgcccgacg agcgccgctt ctaccggttg
    36481 ggcgtgcgcg tccgctgaaa gcgtcgggtc ggggcgttgt caggttttcc actgcgcggc
    36541 atccgccagg cggggcagcc ggccgcgatg gtccacgcag taggcgaacc aggaggcgaa
    36601 ggccagcagg gcctgcggat cgcgggagag attcaggcgg gcgatgaacc gggcctggat
    36661 ccggttccac cggcccaggt tcctcatttt catgctcatc gaactcatgg cggttctctc
    36721 caccggttaa taacgttgtc gcccgtcatt aatttttctt ccttccggcc tccaacggtt
    36781 gagtcggcgg ccggcggccc cttgtcgcgc cgccccggca ataaacggcc cggcccgggg
    36841 gttacgcctg tggaaagcga gggggaacat gaagaaaatc gtatgctggg cggtttgcgg
    36901 gtggatggcg tccgcggcgc atggcatggt gcggctgaac gacgggggac tgtatctctc
    36961 gattcagggc gccgtgaacg cggcgacgaa cggggacacg ctgaagatcg cggcggactt
    37021 ctatcccgag gtcgtcatcg tttcgaacaa gaacctgacg ctggagggcg ggtacaacgg
    37081 tgcgttcacc gcccgcgacg gcggcgactc cgtcgtggat gcccagcagg ccggtacgac
    37141 gctgtggttc atcgactcca gctcgcgggt ggaccgggtc agcctgacag gcggcacggg
    37201 ccacccccag aacctctggt gcgggggcgg aagcctgctg caccggtcgt ggatcgagtt
    37261 cacggactgc cgcgtgtact cgaacgccgc gacgttcggc ggtggtctct tcgtgggcct
    37321 ggacgcgtac gccaagctca cgggcgcaac ggagatctac tccaacgccg cggtctggtc
    37381 cggcggcggc gcgttcgtgg acgggcgctg cgacctcgtg cacagcaaca cgctcgtgta
    37441 tggcaacacg gcgaacgagg gcagtggagg cggcatctgg gtcgagaccg gttacctgcg
    37501 tctattccaa ggctgggttc gtgacaacct cgccctgccc ggcggctcgg gcgcggccgc
    37561 ggcgggcggc ggggtggggg cgtcgggctc gttcgtcgag atcggcgacg gcgcggccct
    37621 gatgcagaac gaggccgtcc tgggcggcgg cctggccctg ttcaactcga ccgggcacct
    37681 cgggcgcgcg cggattgccg attgctacct cggcgtcaat tcggccgtgg agcgcggggg
    37741 cggcctgtac gcctccaact cggtcctgct ggcaaaggga ttgtgctgtt ggggcaacga
    37801 cgcgattctg gccggcggcg gggcctggct gcattcctgc acggtgaact cggacacgaa
    37861 tggaatgggc gc
//
LOCUS       DAOOTK010000022        36933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_829_length_36933_cov_5.455312, whole genome shotgun sequence.
ACCESSION   DAOOTK010000022 DAOOTK010000000
VERSION     DAOOTK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 36933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36933
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_829_length_36933_cov_5.455312"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PK634_04405"
     CDS             complement(<1..1187)
                     /locus_tag="PK634_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97010.1"
                     /translation="MAGLIPAVFCITNASAADPATPPPDPAQQAVDELSKKSGVKIQQ
                     DGIGNPGGRDIDTKKLTADEYRRVRAAAKELGYNVSERGDSMTITGKDGKPIKEITAH
                     ASDNNADPVGSSARDARNISGDDESFRGRIDPKTGKPAVDPKTGLPKPTVNDILGKKL
                     ADGGHALTKPPAQMTQDDLQNLGKTAKKVMDIGEVKDPVFRKQAEILHKTGDPAQAGI
                     KDINEWQQQAKQKAVEAVRNEEAQIRQEKSQIKRDVDQAKENLKKAQASKDSGKIQAA
                     KNKLLDARTRAIEYNSHLNGTTQKAINHGAGEVIAEANGYQKTTTPEGKTRYVNPQTG
                     ERLTQSQFDERMTKGKQIKLTSSPLPTGKTPAPTAPKPPSTTTKIGGTILLLYGAYHA
                     VQEA"
     gene            complement(1220..1996)
                     /locus_tag="PK634_04410"
     CDS             complement(1220..1996)
                     /locus_tag="PK634_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97011.1"
                     /translation="MNTKGIASLVLLAAILRAFGQEVLPEDANPFPGEATAVEAFNRN
                     PAEETLAALTEVRLQNAARHMRDHVGTESVDALILAESIALAAVQDDPDHLQARLLLG
                     TIYLLADGTYLPAEELIEEHFESALIIDPDNLFALDTLAKWHFLRGEYDRAIEKLERI
                     LMLGNPYIHPARVDMLCAAYRQDEQSERGARFLAGRLARALGNDTLQLGLALMLLEEN
                     KAGRATEMLERIAANDRALEENRQLARATLEELRTKGRRP"
     gene            complement(2201..6766)
                     /locus_tag="PK634_04415"
     CDS             complement(2201..6766)
                     /locus_tag="PK634_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97012.1"
                     /translation="MNMRKPILLMALMLLASWAAPGAQDAKDKDNHSNWTLLPQPPPP
                     PPTDIDGLRNEYDAKVKNLQDATDAAAKKYQKSDGTVDTENPGYKKLQQDYVNQKTAL
                     RNEYNAKHPAAKAWESLQNTPEGGKLKSSGSNPSSPHSDMDITADTPEAAKAAADHLK
                     GQGHKVTYDPETGMYQDHTADTKIWEPETPKSAAGRAKNPEGYTRSGALEHEGIKSPN
                     AQYDPEGYVEDLNRKYEAAKAKGDVRTMNKIAAKMQEATGKPVDPVSGKMKVDADPYE
                     SGEQNFGESEEVKNAKAQNRIKQLDETVKNARDTAKAQSESNKRMRDDLAKKETELGN
                     KKSAKEYTDANKKIENTTPKSEPPKTEPAKTGGSSTEPAKPPSPPPEPGKGAGAGTGG
                     DKPPATTHEPAKPPSSTPEPGKTGTAPPETVKPPTTAPEPVKPPATTEPVKPPSTPEP
                     VKPPTTTEPVKPPSTPPEPVKPPGTTESVKPPSTPPEPVKPPTTPEPVKPPSTEPVKP
                     PTSAPETPPATTEPVKPPSTTPEPIKPPATTPEPVKGPGTTAEPPTPPATTEPVKGPG
                     TPAEPTTTEPVKAPSTPEPAKPPATTPEGVKAPGAPSTGTGPTPPGETWQNPLTGKSL
                     PVGTSGSKIIDGTAKVLESKGAQGTLNVVGTVMVAKDGIQTINKVNEAAAKGDLKTAN
                     VEGSKGIGRLGGGWLGSIGGAKAGAGIGTALGDGPGAVVGGLIGAIGGGILGAWAGEK
                     TGETVGPAVIDTVRDGGEKIMDKYYGTDTGGTGGGKGGTTPGGWDTSGKPGDFDRIER
                     VTGGANKDKGPSGPTTSGFTPPSVHVSSSGGSTLTPVPSGSGSTYKPPTTSSGGIDIS
                     VPGQDIDDIDFGTPPPSGGGKGGKTPTTTKPPVRTTPPPPPKKEPVKKPEPPKPPPVV
                     KPQPPLPPPEVTQPPGIQVVDKGWVKDGNGVIKVTETKDANGKVIKVTHTEYGPDGKV
                     IKTTDYPGGTGQGVTTPGGQPPVNGPDSTGVTEPPGATTGGQDTATPPAEGTTDQPPP
                     PSGGDITLGGGGLGKPPDAANHSEAYNKLYELGWETERIMELEKIICPKNSAPGFHVV
                     DKAEEAAWEPYRKRLEELGKQRDALLNDPNVSYGDKSAFGSGRWDCAKAIADGRVKKE
                     PFLTAGTTVSNVTGSPAIDDNLQPAIDRMTGRDLRTSEAAALPQESVETSFDRDLPLE
                     TVIQERNTQMEEKSENGVAIMGAHTDVKNASNAGHDMIRDAKYTRDAGGQDAQKIASD
                     NQQKIRIEQDKNKNPILNGVLNGIGNGTKTAAGHLGTGLGKGIGDKIFDSGKKTETAS
                     TGGTSGSTPGSSGGTSGPRVSGGTSGGGPDGESYGGTSGGTTSGGQTAPGGGPVCVKC
                     GAPGYMEFPGVGWLCHDCEAAGYGYPPDGTTGTETSGSQPVIMADALCPVCGQMYNPA
                     YGHNCPGASAPTPPPEPPPAEMDSITCNECGYVHQFPKGTTPPRACPRCGCGPDSDKV
                     TCTACGHSWAVPAGQRPSSCPSCGVSF"
     gene            complement(6786..7247)
                     /locus_tag="PK634_04420"
     CDS             complement(6786..7247)
                     /locus_tag="PK634_04420"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOW97013.1"
                     /translation="MKTWKLSALALILVLASNAAPAGPIAHRQLVEKLMDLFSVETMC
                     QSAIDALVKQAVATEPGLAGSETAIRSFLSKQIGWEVLKPAVIELYMGELTEKELAEI
                     VRFYESRTGRKFAALSPAISARVMEMVQERMKQAEPEWETLVGSLLQGAPQ"
     gene            complement(7406..10393)
                     /locus_tag="PK634_04425"
     CDS             complement(7406..10393)
                     /locus_tag="PK634_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97014.1"
                     /translation="MNAGLSARKTILILGLFFPLSESFASSSDKLDAILDPIADKLDP
                     VNRDLKSNVGWTTRKLINETAKKFPGDSGCKIIAKSSKIISLTDSGSTMAGELYEGKW
                     GQACLTAIDGVGKFFSTAAGTVLGGSVGSSGGPVGTVAGGTTGGFIGKKLWEYTGGQI
                     VSILKNDLYNQQAKTRYAGSKNVVNKPGNDPVAWQKAHAGSKSSNQNQKGSNQGTPKG
                     PAITSTTRPGSTTATGNNTGGTAIPGGNAGNNQGHSQGGTAPPGGNTGNNRSGGSVPG
                     GNNGGGNSSGGTPVPVGNNGSANNQGNNQGGNNNWNTGGNNAGGDNQGENQPGDNNGT
                     GNNQGDNNPGGNNRGGTKDGTKGGNNQPDGNNPGQNQGNPPGGDNEGGVNVWAGNQGG
                     DNNGNQGGIHAGEQNEPQSPVGGTTIVKGWAKNSTGTTKVTETTDSNGNVVSVTHTDY
                     DSNGNVISTTTYPASTGEGQTDTNQDGNNRGNNGQVGGNQTGNQMPAIATSSFLTCAI
                     CGKRFEQGTDGVTSSKMTPLPFNSKLSACENCAKADYLTAPSGCDAKEPGKFCLHCGS
                     KFKEAVTRVKDDDSSRAGYTTEIVCPNCGTVLDIQHQTTVNAINTPDKWSNLETSMAS
                     ASHACPKCGQAMTSVLEEHPEYLSCSPPEPSKVNQWRDVCPNCGFRTMILSEAEIRDL
                     GNGKIKAETLAEDETDQSLSVADMRKSEAESLKDTEAISISRPEVLSGMIGQQNERMA
                     KKYDTGLEVMGNNTAIDKASNSGNETLKNAKITLNAGGEEAVKIGTENQHNLRIAQNN
                     TKNILLNGVVNGVNTGVKTAAGHLGENLGGKAGDSIFGGNNKGTNTGNTGRTNTGTDH
                     PPVGPQTVNSSSNTNITSYNGHNYEPDVGGETNTETTTSSSTVDSGPVCVICGGPAYM
                     EFDGVGWLCHDCEKKGYDYPGGPSGNTGTSDGTSSDTTDTTDGSETDTTVEGGDNPGG
                     IDSQTTPTSVPDDSPDLYPPPVP"
     gene            complement(10390..11526)
                     /locus_tag="PK634_04430"
     CDS             complement(10390..11526)
                     /locus_tag="PK634_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97015.1"
                     /translation="MQHPSSRRMLLSLSFLVAPMFGAINSTIWPSAYAQQPEHDAWPN
                     AQDATAHDDRAQDPLSFQEIEGLLLKRVAVFKSACVASPAQAFEDIMGNEARADLTRI
                     NSNTFDKSDSDKLWSYFFNTSVFSLGPSEGASMKVLFFHPWSDTALLTLWTFREDRTE
                     ISRLQFVPAEYLRKGGKLPISMSPLWRQLAAHITPVLAVQWGAVQTIEAFEGLPSTVW
                     VPSPTGAEATSEQVEETLSAAAGIQLGQSYGAVAKLLNAPEYEALRKEIDRCAEDIDH
                     GLATCTSPWRQSLDSRTLDYLEPLQGKIDSFSPAGFLTDGTESIVFMTCDSQPSLGLS
                     LSFQKTDAGFQFQRVGYLDVPTVFTQRKEIQAQLPSAVLEEEQP"
     gene            11858..13177
                     /locus_tag="PK634_04435"
     CDS             11858..13177
                     /locus_tag="PK634_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOW97016.1"
                     /translation="MNKWMIVFMGLLLAAGSALAAEVDLFEQAPWSGGVGLDFMHFEG
                     DQAVEDSAGLFLKFNYAFSPRWSVDLAADLFPSLPNREFTTERPYQLDDDTWALRLGA
                     DGLLHLRTIEDLRWDPYLAAGAGLMIFGDDPSGGQVQPALDAGGGMFYHFNDEWALRG
                     DLRAHTTTLNWEFQALASVGVMWRWGARVPPALQVSGGALDSDGDGLLDTEEQQIGTD
                     PFDPDTDKDGLTDYQEVRETQTDPLNPDSDSDGLTDGAEVNVYTTNPLDADTDDGGVR
                     DGHEVIEDGTDPLNPNDDLQLFTLYIEFDYDKSILKPQYFEKLDVIVKVLQRDPGATA
                     RVEGHADKRKTSQHEYNMKLSERRAKAVVDYLVNTGGIDRTRLVYKGYGFTRPVAPND
                     TEENMQKNRRTEIYIRPSGNVPAVSTTQPMVVPAAEGETPAPAEPAK"
     gene            complement(13233..13427)
                     /locus_tag="PK634_04440"
     CDS             complement(13233..13427)
                     /locus_tag="PK634_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97017.1"
                     /translation="MKYTAQLESSDGEFVASCPELDISAHGLSADNALDALRRNIRFH
                     VELCPCSTVEEDSIELDVRW"
     gene            complement(13505..14647)
                     /locus_tag="PK634_04445"
     CDS             complement(13505..14647)
                     /locus_tag="PK634_04445"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOW97018.1"
                     /translation="MQPGIETLLIHHLDRLRGRRVGLVAHPASVDSNGAHSLQRMLDA
                     GVNLVALFGPEHGFTGRGGAGEVIADGREPVSGLPIRSLYGDTRKPTPEMLCDLDAVV
                     FDLHDLGARPYTYVSTLRYVMEAATDNGKAVIVADRPIPLPRVVDGPMLDPAFESFVG
                     FVRTPVSYGMTPGETARFLREDLCLDLDLFVAPMRGFSRNAPSPTGRWIPPSPAIRSW
                     ACAAGFPATVFFEALPSLDHGRGTDQAFQVVGAPWLDALALADGLARHPLPGVRFEPC
                     SYRAEGAALKGLEVRGIRLDITDPAAFRPVQSGVTLIAILQELYGPEALWDAPGARPE
                     FFDKLMGTDAVRRALLDGAAPEAIAAGWPKRQIAFLETRQASLRYT"
     gene            complement(14748..15731)
                     /locus_tag="PK634_04450"
     CDS             complement(14748..15731)
                     /locus_tag="PK634_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOW97019.1"
                     /translation="MNLDVFLATLLVLDSNPPTFDASPARRDAFLKIGEFIENNTRAG
                     APFDLKTSPNPFWQAIETRLLQALDGIEAEPADAPPTAWQLYNDGVVLTADGLAIGMD
                     VFPFPRRFGWTDSADLTGRLADLLDVLFITHRHPDHYDKALVRACLERGKPVVLPAPL
                     AADWPGVKTVIAADDGLAFKVAGLDVTARRGFHVWRATMEDLPLVYYEIVCPGDFTFL
                     FIGDLDVSKKLEKTAGRDIDLFFVPWRHPNETYEPGHAAQTGRTYDAVQAALDKVRPR
                     ALLFEHLAELEHVYDGFPASYDIAIDLKARVRVPSELLFWGERIAWPSHPR"
     gene            complement(15728..16573)
                     /gene="panC"
                     /locus_tag="PK634_04455"
     CDS             complement(15728..16573)
                     /gene="panC"
                     /locus_tag="PK634_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOW97020.1"
                     /translation="MQIVRSPADMQARSLALRREGRRIGFVPTMGYLHDGHMALVRLA
                     RARSDIVVVSIFVNPTQFGPQEDLAKYPRDFERDEALCREAGTDIVFYPEAKDIYLTG
                     HSVYVVEESLSRGLCGATRPGHFRGVATVVAKLFNIVLPDVAVFGEKDGQQLRVIRRM
                     VRDLNFPVEIVAGPTVREPDGLAMSSRNKYLGPEERRQALCLRRALDRAEALTRDGVR
                     ETGSIRAAMQQVIAEAPLARVDYIEIVDDATLEPVKTVARPAMAALAVFVGKTRLIDN
                     TVLRP"
     gene            complement(16606..18255)
                     /locus_tag="PK634_04460"
     CDS             complement(16606..18255)
                     /locus_tag="PK634_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOW97021.1"
                     /translation="MKQVATFVLLAAWLAARAQGDCLPPDCIAASTNPPGGLAPLNTP
                     QMILLSWDDSVTTDSYALVQSVLTNHVNPNGQPIKATFFVSLDSRIDYSLAQRLFADG
                     HEIAVHTMTHTTGTNTLPDTWRREIVGCRSTLSALGGIPEAAIVGFRAPYLFSNDGSF
                     RILAERSFLYESSLFEDLAGMSPSPAAMTWAYTLDDGAVENVPAVRQPAQPYPGLFEL
                     HLYDLFSTNLSPVTIMDPPEGYDSNAVMNLWKTNFLWHYDGNRAPLTLALHATTTNQW
                     MSNPAHSAWRIAALNDFVEWTLAYPHVYFVAYRDLAAYMRDPVDVSAAPTAAVFQTAR
                     VSPQTNLSACAYPGYRTLRVIGGCPPAYPAPTNLYWQFAPTSGGTVAFNVISQDATHT
                     YAYFVVTNDTAGTIYHWNTAVDISAGQVSWLWDGFWTQTLAHLDVHAKQYNRILAPGG
                     SATVTFRLDTTNPVLFTGASVALDALAPATPTLATIRPDAGSSRVALAWDRTALEYEL
                     HAATQLVGEGWGLSATGVFTEAWTGEVAAPGPVFLRVGGRH"
     gene            complement(18252..19055)
                     /gene="panB"
                     /locus_tag="PK634_04465"
     CDS             complement(18252..19055)
                     /gene="panB"
                     /locus_tag="PK634_04465"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOW97022.1"
                     /translation="MNTSPWTAPKIKALKGKEKIVCLTAYDYAMARLVDAAGIHLVLV
                     GDSLGMTMLGYENTLPVTMDDMIHHTAAVARGVKTALVVGDMPFLSYHASIEQAILNA
                     GRFIQEGGANAVKIEGGAFREPLIHALVQNGIPVLGHIGLTPQSIEVMGGYKVQGRNH
                     EGAKQLLEDARAVEEAGAFALVLEAMPSDLAAEITATVKIPTIGIGAGPACDGQVLVL
                     HDVLGLYGDFKPKFVKRYADLGAQVAQALAQYRQEVQSGAFPGPEQSYE"
     gene            complement(19065..19568)
                     /gene="folK"
                     /locus_tag="PK634_04470"
     CDS             complement(19065..19568)
                     /gene="folK"
                     /locus_tag="PK634_04470"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOW97023.1"
                     /translation="METIISMGSNLGDRLAALTMARRRIAEWPDVHIAAQSPVYETEP
                     VGVLEKYSHLPFLNAVLVLESPWDGHAWYQRLHALEAKLGRKRGLDRFAPRTIDLDII
                     CVGSTSLQSGGLAIPHPHWKERRFVVQPLAEIRPDLVIPGETRPVRQVLAELPAEPRV
                     ALFAKEW"
     gene            complement(19556..20461)
                     /locus_tag="PK634_04475"
     CDS             complement(19556..20461)
                     /locus_tag="PK634_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97024.1"
                     /translation="MPSTLVHLGAQGIVSKAVFRKADLKWVFLGLVIPDVPWILQRAI
                     RVLPFTPNPYDLKLYGVVQSSLAFCLLLGAALALLSSSFWKTWAALAFNSLLHLLLDA
                     AEQKGGNGVFLFAPASWRMTDWDLLPAEGWAVTLLTALGVAVGLWGLYQQVRAPAGPI
                     PPLRSRLLLAAAAGLAAYGLLPLAFIEGPLEADAFSIRTLKDVERRPGRAAAFDRAVF
                     LPEDGGRLRLFSGEVLRVEGISLDRQALVTAGGVFLAPDRFRADRWVAHTPRYRDAAT
                     GLGLAFGAAMWLHAGIRRMRRSWKP"
     gene            complement(20493..24053)
                     /locus_tag="PK634_04480"
     CDS             complement(20493..24053)
                     /locus_tag="PK634_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOW97025.1"
                     /translation="MREIPYNYTSADDGQVIRFLFGAETWARWEGLRAKRVTGRSARL
                     LLRVIGDLFVLKRNPFMVQDLLDHPRRQAAYFDEMRSELKTVRAGAGPNEDVLRIVDQ
                     CERVADELATALAAAPARRAQIRRRLGAIIGEENVLFDPLALVCHATDATDWRLCLPA
                     AVARPGREEEVAPLLAAIRGLGLHAIPRGAGTGLTGGAVPLREGCVMINTEKLNRIRG
                     IAGNALAVEAGVITDDAIEYARRRGKVFATDPTSSWASTIGGNLAENAGGKTAVLWGT
                     AIDNVLRYRMALPDATNLEVRRRDHPGRKIRPDDTVEFDVAGADGRILRTIALRGSDI
                     RKPGLWKDVTNKALGGLPGLQKEGTDGLITSAEFILHEPYPQRRTFCLEFYGDDMDEA
                     SRVIVDISRAFEAAGGEALMALEHFDDQYVRAIGYKNKAPRAHPPKAVLLVDLVGHTP
                     EQLADGTRKLEALLRPYPNTCLFAARDDAEAARFWQDRKKLGAIAAHTNAFKLNEDIV
                     LPLEALARFARYVDERNNDENRRNQQWLTAEFGRRVESAAVAEDAERYSGKIPRARQL
                     CAEAAEQLARAPAEDLRSERIIRELRAGLGQLVSGYRQIEADLDAAERHVRSRRIVLA
                     THMHAGDGNVHVNIPVFSNDRDMMERAAREADGAMRTAVAMGGAVSGEHGIGITKLKH
                     LEPERIRELDEHRRRLDPDGLLNPGKLQDAGALDAVFTPSFNLLELEARILQHGSLEE
                     LAQKIAKCVRCGKCKADCCVFYPARNLFYHPRNKNLAIGALIEALLYDAQRSRSTRFE
                     SLRFLEDIADHCTACHKCLKPCPVDIDTGEVSILERNILKTRHFKHTPAPTRLTLAYL
                     ESRRPLFNRLFRAGVLRAGGACQRLAHRVASGLPKSLIQRVPGHALLDSPMPPAPPGT
                     LRDRLPPCRDNQTLCFRPEGAARASVFYFPGCGSERLFSDVSLAALYLLVKTGVQVVL
                     PPPFLCCGFPAHANADGERHNRIVLHDTIVFSQIRDMFGYLRFDACVATCGTCMEALR
                     AMGLEAVVGCPLRDVSEFVLAGGAEIPGGGAPALYHAPCHDSLAGSGPALLARGGAGP
                     VEPVPHCCSEAGTLALSRPDISSSMLQRKADALQAASEKAGTKLVLTNCPSCLQGLGR
                     NRPRGIEPRHLAVEFARRLGGPEWEAELEPFIRSAEVTTF"
     gene            complement(24147..25256)
                     /locus_tag="PK634_04485"
     CDS             complement(24147..25256)
                     /locus_tag="PK634_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW97026.1"
                     /translation="MVKHVARGGGIERYTEELGSRLARRGHAVRVYSTRHYGALVPEY
                     KGMRIVGVPSLPLTACEKLSAGLASVLHASLSPWADILHLQSIGPGAMGWLPRLRRIP
                     AVVQFHGIEWRRARWSRFGSAVLKGLERSTVRCNRLFTAASQTQCAYFRDAYGIEVRW
                     IPGGAEVKTAPEPREILALGLEPRRYVLFASRLVPDKGAHYLVEAFRRLDTADRLVIA
                     GDVRGAEAYREELRRRAGNDPRIVWPGFVQGRLLEELVGHARVFVLPSDLEGMSQALL
                     EAMGYGTCCLVSDIPENMEALGDAGAAFRRGDAGDLADRLRALLTDPSAAEEYGARAA
                     ARVREQFSWDQITDQFERWYEDILRAAGRRKRRRA"
     gene            complement(25249..26469)
                     /locus_tag="PK634_04490"
     CDS             complement(25249..26469)
                     /locus_tag="PK634_04490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW97027.1"
                     /translation="MKICYLNNHLYLRSGAERVMFTEAALMRQAGHEVSFFGCRGPDD
                     LAREHAAFYPPRVVIERLQGLGKWRHAFRVVYNPAVGRAFRRFLDAVRPEILHAHNIY
                     GGLTTAVLDAARDAGLPVVLTAHDYKLVCPSYLALDHGRVCTACKGGRFYRCLLKRCH
                     KSSLAASALYTAEAYLAAWGRKYAVVRRFICPSRFLRQTFLDNGRAPERVAYVPNHVA
                     TDSIRPSFGEGSYALFVGRLSLEKGLTTLLRATEGLGIPLRIVGEGPLRGGLEDQAVR
                     NGRRGQVVFAGYRAGGDLAREYRDAAFLVIPSEWYENAPMVVLEAFAHGKPVIGAAMG
                     GIPELVEPGKTGLLFPPGDVEALRQALASLWQNRASWGALGAAARRRAEEEFTPDRHR
                     KQLLAIYEDCLRCG"
     gene            complement(26466..27659)
                     /locus_tag="PK634_04495"
     CDS             complement(26466..27659)
                     /locus_tag="PK634_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97028.1"
                     /translation="MASYCLYVLFTFLNEATGGERQMVGAVLFLSVVAACSARRLLPV
                     PLTGLTVALLFSALLPMVALLSGLTAGNEGAPAYTIKYFSLLFVLLAASSLRLPPLYR
                     TPERWWGLVAILSILLIGLARGSGEDRVEGSFANPNNYALVALSLLFFVDRVRDARWF
                     IIGLYALVLALVILSGTTGALMGYMAGLAVALLCTRFARLTCAALAGAALLGALLLAT
                     LHTIDPAALAEMRIVGPLWSKVYIAQQHYADIAAGSDLNFWELGTEYGNVEYTSSIWR
                     LAHWHDLLWKFRRSGVLSRLLGHGIGSSPVFLGKLPHNDYLRLLFEVGVLGLLANLAA
                     WLILFRRAEPSARGPAAMMAAYAITENNLDNFLVMSLFALFLVSARRAIVPPPIPAPP
                     GRVFS"
     gene            complement(27739..29961)
                     /locus_tag="PK634_04500"
     CDS             complement(27739..29961)
                     /locus_tag="PK634_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOW97029.1"
                     /translation="MSTEPSPEQKPASLYGASLYYDHLYGAAPAGLALLDPRRLLRKW
                     RTLTLTLAFAVVAALGYLWAAEKIYRANSLIELSVRRPRILTQQPAVIEDQAGAQQST
                     EVFNTRLEKFKGRTMLEAALQRLDAVLPGAFAPASPASSDSPELRHDKRLKRFEKALT
                     LTLVRRSRLIRVEFEHPDPKVATAACNAFADAAEASAYEENRTTSDAAVAWLEAQAEL
                     QRKDLLKAEDALLAFRQEHKIDALESERKTVDDALQGFNKSLVDVESREAQESALLAK
                     LEQLTLDPERAGEFPADIPRAEEIRSALEQWRVAVVERNSLLAAFTPKHPEVQAKDNV
                     VALYREEALKALERAKTTARSNNNLLREQAETLRRKKDEQIQLAAKLEMQVVERKTRL
                     AALERSRDAADQSFRGILARIQDARMAADENTATVKIIDRATVPLKPVHPNPLHTLAL
                     ALLLGFVGGLGLIVALDFLEDRVTGPEDMEGRGIPILAVVPHVRNADRASIATATIRQ
                     HFSEVVEAFAGLGTMLDSPQHKERSQVILIASSIPGEGKTVASCNLAAILAKKGRRVL
                     LVDFDLRRPRLAGIFPIPPGRPDLLTVQPEANPRMAGLPYPVADCPNLEVIASRPMSQ
                     SNPAVALGALAGALISWARTKYDHVVLDAPPLGLVSDTLALAPLADFTLVMVRPEVSR
                     KRLTWHTIHRMRESGIHSLGLVVNDLDVSKMLYNTYSPYYHYQQHYKAYAPAEENGGG
                     "
     gene            complement(29977..31422)
                     /locus_tag="PK634_04505"
     CDS             complement(29977..31422)
                     /locus_tag="PK634_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOW97030.1"
                     /translation="MSTRTVPAPTPPGCTETRDGREKCLAWGLLLFLSVAAWRWAGLR
                     AADGPFFAAAAALLLAAALVARPRSWWSRDPFFWFGLVFLAYLACQRWNAGRELRFDD
                     AIRRWAYTDPPHPRWPWAFSRPEAAQMLHWFFPAWAAGLCVRCPALSRRGLRPVLHGL
                     AYNAAALALFGTVRELAGAQSLFGYRPAYADFFATFTYTNHAAAYFLLMAALAAGLLF
                     RQVFRRDRPGRRIEKAALAASLLLCLVGANLSLSRAGIILSWALALFIAGYGLARGWR
                     LWRPAARLRMAVLTAAAAVVFVLTVAGAGGEAIRREFTLRRRPAHQAVPGLEQVNLDL
                     SDRPRLWGAAWAIFRDHPWYGTGGWGFRYLAAFYLPRDHWSSLKSNPGRANAHCDPLQ
                     FLVEFGIAGAGGLALAWGALAAGLFRRDLARGALFTLSCAGLTLVAAFSLIDLPFRCP
                     SVLWTWTVVLAALPKCTAREPREVGAAAVKH"
     gene            complement(31432..32088)
                     /locus_tag="PK634_04510"
     CDS             complement(31432..32088)
                     /locus_tag="PK634_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97031.1"
                     /translation="MKTRTAIGLAALALGVATLAASAAFTPPTDEQLNAAAAAPGSKL
                     AALLEEASPDQVVDVVKSVAEKIIGLNLAPEEQAARLADVIRIAFKALPSEEFDVFAA
                     GLGKAVANSAVISGSAGAVTAMRDALGSEGGGMVSSFDQLFLASGGALPPVPHPQPPE
                     PEPVIEEPAATTPAATTTTRTRTRTRTRTTPTTTTTQEEEEEEEPPPPAPGYEGQNLP
                     "
     gene            complement(32112..33308)
                     /locus_tag="PK634_04515"
     CDS             complement(32112..33308)
                     /locus_tag="PK634_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOW97032.1"
                     /translation="MKTKWMGLVLAIGLLAATGWGQVQPGFHAGPLTLSPFAGVAGSY
                     DSNTGRTSSDEQDDIFVESDVGLRLDCQVREVQISGLGFVVQRSYADEDGLDFWAGGE
                     ALTLRIGNPEKITLEAQESWRRVEDLDTYGSAADIGGMSPDAFLDADVRSRREVLEAG
                     TVLTAQLTDKSDVSAGYRYNQTDYRSDDLFDLSGHGGQMEAAHRVTDKTAGLLTLVGG
                     LQESDDVDGEAEYFAARLGVRTRGTDRMSLKAGAGFQQLDRPEDAGTEDGLNFDAVAT
                     WAATDKTSVELEGRNGIQMSSIHLANIVDFSVFRLGVAYRMTPSIGLSASGVYRIDDY
                     ADPVMDEGELVDREDEGVTVKLRADYLIPAKYLRFFGELMYETVDSTIRDYDVGRVNL
                     GVDIGY"
     gene            complement(33322..34041)
                     /locus_tag="PK634_04520"
     CDS             complement(33322..34041)
                     /locus_tag="PK634_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOW97033.1"
                     /translation="MLQAGKEWRTRSLVLALAGGVLLAMATGCASRRAAKAARAVKPP
                     PAAPAPAHSTALPKPNGAEQATLRPGLMVNVTVIVAGKKQIEEAGRRVSETSTLTLPL
                     LGSLAVGGETLDSLTARLTAEYREYYVDPQVMVEFVRDDQPESISPWGSITVLGRVKR
                     PGRVSIPPTRDLTVSVAIQQAGGFDTSAKDTAILVTRRAPDGHTTTRHINLREVGVRG
                     RVENDIVLLPDDVVYVPELVF"
     gene            complement(34158..35195)
                     /locus_tag="PK634_04525"
     CDS             complement(34158..35195)
                     /locus_tag="PK634_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOW97034.1"
                     /translation="MKKRILPALLTLLIALVAAGAIGEFAVRRLGLAPPLPEQFKNYA
                     STPAMPYGPKPHSRASGRSFKGEFEYDYRHNAFGLRDGEHELSKPPGVFRILGLGDSF
                     TYGVGAAFEETYLHRLEQMLNTRPGDHPRVEIIKAGIPRYFPEPERILLEKLGLQFQP
                     DLVIVGFVPNDVIDTFYGLDAVTVDPSGYLLTRQAASPGAVGRFLYKRSHLCRLVLAR
                     FMAWRNDRVENWAAVYEAGGAHEADWRRIEDEFARMAALAGSIGARLVIAHVPRKGPW
                     TDAHDYPARRLAAWAEANGAGFVDTRPAMRAAPSAPPLYYPKDGHCTPTGYAMVAGAI
                     ARGLEEGGWVP"
     gene            complement(35192..36457)
                     /locus_tag="PK634_04530"
     CDS             complement(35192..36457)
                     /locus_tag="PK634_04530"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOW97035.1"
                     /translation="MNEKPASIRDVVGARLCTGCGICENVCPGGAISMTRRGGLYEAA
                     VEPDRCTGCGLCRQVCPGPGVDRSRLGAALFPEDAEVPGLGRCAGCYVGHCADDATRR
                     LGSSGGLVTGLLAWLLKTRRVDGAVTIRSVPGRPLEPEAAVIRDPGDLPAASGSRYCP
                     THFAGVLREIEPGPGRYVFVGLPCHLHALRKLQEARPRFRERIPWAFGLCCSGTRTFD
                     ATEYVLDRMGIAPASVVSLDYRRGDGLGAMRITCADGRTRTREYVEYYPALRSFFIPH
                     RCALCPDHFAELADLAFGEMRVPEFPDHRAGTGAVVARGRRGRGLLEEAAAGGAIRLL
                     PIDRARVLKAQASHLRRKKRDVPARRKLFRRLGVPVPRDDEPAARPCVRDTLAAWRAT
                     LILYTERAIGRRRALWWMIDRLHGGKRAS"
     gene            complement(36454..>36933)
                     /locus_tag="PK634_04535"
     CDS             complement(36454..>36933)
                     /locus_tag="PK634_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOW97036.1"
                     /translation="PHQAYPAGFPGLTPETRLSWDGDDRAAAELVLDAMSDRTGVYAL
                     AGDYSPAEYKGLLKRCELFVGGRMHAVISAVSAGTPSVIMQYSHKASGVMEGLGLQEW
                     VWDIRAPEADLAGLVEKAWAGRAALRARLAERSGRDAEEACRLGDVFAEALQARSCA"
BASE COUNT         5529 a        12142 c        12681 g         6581 t
ORIGIN      
        1 gcctcctgca cggcgtgata ggcgccgtac agcagaagga tggtgccccc gatcttggtg
       61 gtcgtgctgg gtggtttggg ggctgttggc gcgggggtct tgccggttgg cagcggcgag
      121 gaggtcagtt tgatctgctt ccccttcgtc atgcgctcgt cgaactggct ttgggtcagg
      181 cgctcccccg tttggggatt gacataacgg gtcttgcctt caggcgtggt ggtcttctgg
      241 tagccgttgg cctcggcaat gacctccccg gcgccgtggt tgatggcctt ttgcgtcgtt
      301 ccgttaagat ggctgttgta ttcgatcgcc cgggtcctgg cgtccagaag cttgtttttg
      361 gccgcctgga ttttaccgga atccttactg gcctgcgcct ttttcaggtt ttctttcgcc
      421 tggtcgacgt ctcttttaat ttggctcttt tcctgccgga tctgggcttc ctcgtttcgc
      481 accgcctcca cggctttctg tttcgcctgc tgctgccact cgttgatatc cttgatgcca
      541 gcttgggcgg ggtcgccggt tttgtggagg atctccgcct gcttccggaa aacgggatcc
      601 ttcacttcgc cgatatccat caccttcttg gcggtcttgc ccaggttctg cagatcgtct
      661 tgggtcatct gcgccggggg cttcgtcagg gcgtgcccgc cgtccgccag tttcttgccc
      721 aggatgtcat tgaccgtcgg tttgggcagg ccggtcttgg ggtctacggc aggcttgccc
      781 gttttggggt caatcctccc gcggaaactc tcatcatctc cggaaatatt gcgggcatcg
      841 cgcgcactgc tgccgacggg gtccgcgttg ttatctgatg cgtgggcggt gatctccttg
      901 atcggtttgc catccttgcc ggtgatggtc attgaatcgc cccgttcgga tacattgtag
      961 ccgagttctt tggcggccgc gcgaaccctc cgatattcat ccgctgtgag cttcttggtg
     1021 tcgatatcgc gcccgccggg attcccgatc ccgtcctgct ggattttcac gccggacttt
     1081 ttggacaatt cgtccacggc ttgctgtgcg gggtcggggg gaggcgttgc ggggtcagcg
     1141 gccgacgcgt tggtgatgca aaatacggcc gggatcaggc ccgccatgaa cagaatccga
     1201 cgccctgcgg atgcatgctt cacggtcgtc gccccttggt tcgcagttcc tccagcgtgg
     1261 cccgcgccag ttgccggttc tcctccaggg cgcggtcgtt cgccgcgatg cgttcgagca
     1321 tctccgttgc gcgaccggcc ttgttttcct ccagcagcat caaggccagt cccagttgga
     1381 gcgtgtcgtt gccgagggcc ctggccagcc gcccggccag gaagcgcgcg ccgcgctcgc
     1441 tttgttcatc ctgccggtac gcggcgcaca gcatgtccac gcgggccggg tggatgtacg
     1501 ggttgcccag catgaggatt ctttccaact tctcgatggc gcggtcatac tcgccgcgca
     1561 ggaaatgcca cttggccagg gtgtccaggg cgaagagatt gtccggatcg atgatcagcg
     1621 ccgactcgaa atgctcttcg atcaactcct ccgccggcag gtaggtcccg tcggcgagca
     1681 ggtaaatggt tccgagaagg agccgggcct gcaggtggtc cgggtcgtcc tgcacggccg
     1741 cgagggcgat ggattccgcg aggatcagcg cgtcgacaga ctctgttccg acatggtccc
     1801 gcatgtgccg ggccgcgttc tgcagccgaa cttccgtcag ggcggccagg gtttcctcgg
     1861 cggggtttcg gttgaaggcc tcgacggcgg tcgcctcgcc aggaaaggga ttggcgtctt
     1921 cgggcaggac ctcctgcccg aaggcacgca ggattgcggc cagcagaacg agagaggcga
     1981 tccccttggt gttcataaag ggcgactcgt ttcgcgtaat gctaggaatt gaggggggat
     2041 agtcaagcga aaaagagcgg aacctgttaa taatgaggcg gtaacgagcg cccgcatgtc
     2101 gcaaatgtct atcgggcaat gggggtatgg cggcgctcga taaacttcgc ccgccgccgg
     2161 cggacgcgcc tcttcgccag gccggcattc cagttcccgg ctagaaactc acgccgcagc
     2221 tggggcagct ggaagggcgt tgcccggccg gcacggccca ggaatgcccg cacgccgtgc
     2281 aggtgacctt gtcgctgtcc ggcccgcagc cgcacctcgg gcaggcccgg ggcggagtcg
     2341 tgccctttgg aaattggtgc acgtacccgc actcattgca ggtgatgctg tccatttccg
     2401 ccgggggcgg ctcgggcggc ggggtaggcg ccgacgcgcc cgggcagttg tggccatagg
     2461 cggggttgta catctggccg cagaccgggc aaagggcatc ggccatgatg accggttgcg
     2521 acccgctcgt ttcggttccc gtcgtcccgt cgggggggta tccatatccc gccgcctcgc
     2581 agtcgtggca cagccagccg acgcccggga actccatgta gccgggagcc ccgcacttca
     2641 cgcataccgg cccgccgcct ggggccgtct gcccgccgga tgtcgtccct ccgctggttc
     2701 cgccgtaact ctcgccgtcc gggccgccgc cggacgtgcc gcccgagacg cgggggcccg
     2761 acgtgccgcc ggaggagccc ggcgtggacc cgctggtgcc gcccgtgctg gcggtctcgg
     2821 tcttcttccc ggagtcgaat atcttgtcgc cgatgccctt gccgagcccg gtgcccagat
     2881 gcccggccgc cgtcttggtc ccattgccga tcccgttcag tacgccgttc aggatggggt
     2941 tcttgttctt gtcctgctcg atcctgatct tctgctggtt gtcggaggcg atcttttggg
     3001 cgtcctgtcc gccggcatcc cgggtatatt tcgcgtcccg gatcatatcg tgcccggcgt
     3061 tcgacgcgtt cttcacgtcg gtgtgggcgc ccatgatggc cacgccgttt tcgctcttct
     3121 cctccatctg cgtgttccgc tcctggatca ccgtctccag agggaggtcc ctgtcgaagc
     3181 tcgtttccac ggattcttgc gggagagcgg cggcctccga tgtgcgcaaa tctctccccg
     3241 tcattcgatc aatggctggt tgcaaattgt cgtcaatggc cggactgccc gtcacgtttg
     3301 agacagtcgt gccggccgtc aggaagggct ctttcttcac gcggccgtcg gcgatggcct
     3361 tggcgcagtc ccatcgcccc gagccaaagg cggacttgtc gccgtaggag acattcggat
     3421 cgttgaggag cgcgtcgcgc tgctttccga gctcctccag ccgcttcctg taaggctccc
     3481 aggcggcctc ctcggccttg tccaccacgt gaaagcccgg cgcggaattc ttggggcaga
     3541 tgatcttctc cagttccatg atccgttcgg tttcccagcc gagttcatag agtttgttgt
     3601 aggcctcgct gtgattggcg gcgtccggcg gcttgccgag cccgcctcca ccgagcgtga
     3661 tgtcgccgcc ggatggcggg ggcggctggt ccgttgtgcc ttcggcgggc ggggtcgcgg
     3721 tatcctgtcc gcccgtagtt gcccccgggg gctccgtcac cccggtcgag tcaggtccgt
     3781 tgacgggtgg ctgcccgccg ggcgtcgtga cgccttggcc cgtgcccccc gggtagtccg
     3841 tcgtcttgat cactttcccg tccgggccgt actcggtgtg cgtgaccttg atgaccttgc
     3901 cgttggcgtc cttcgtctcc gtcaccttga tgaccccgtt gccgtccttg acccatccct
     3961 tatcgacgac ctgaatcccc ggcggttgcg tgacttccgg cggcggaagg ggcggttggg
     4021 gtttgacgac gggaggtggc ttcgggggtt cgggcttctt gacgggctcc ttcttgggcg
     4081 gcggcggagg ggttgttctg acgggcggct tggtagtcgt cggcgtcttt ccacccttgc
     4141 ccccgcccga aggaggcggc gtgccgaaat cgatgtcatc tatatcctgg ccggggacgg
     4201 agatgtcgat tccgccggac gacgtcgtgg gcggtttgta cgtgctgccg cttccgctgg
     4261 ggaccggcgt gagcgtgctg ccgccggacg aactgacatg gacagatgga ggagtgaatc
     4321 ccgacgtggt cgggccggaa ggccccttgt ccttgttggc gcctcccgtc actctctcga
     4381 tccgatcgaa gtcgccgggt ttgccggagg tgtcccatcc gcccggggtc gttccgccct
     4441 tgcctccgcc cgtgccgccg gtgtcggtgc cgtagtactt gtccatgatc ttttcgccgc
     4501 cgtcgcggac ggtgtcgatc acggcggggc cgaccgtttc gccggttttt tcgccggccc
     4561 acgcgcccag gatgccgccg ccgatagccc cgatcaggcc gccgaccacc gccccgggtc
     4621 cgtcgcccag agccgtgcca atgcccgcgc ctgccttggc gccgccgatg ctaccgagcc
     4681 agccgccgcc gagccggccg atgcctttcg agccctcgac gttagcggtc ttcagatcgc
     4741 ccttggccgc ggcctcgttg accttgttga tggtttgaat gccgtccttg gcgaccatga
     4801 cggttccgac gacatttaag gtcccctggg ctcccttgct ctccagcacc ttcgcggtcc
     4861 catcgataat tttactgccg gatgtgccaa cgggaaggct cttgccagtg agcggattct
     4921 gccatgtctc gcccggcggt gttggtcccg tgcctgtgga tggcgcgccc ggggctttca
     4981 caccttccgg ggtcgtggcc ggcggctttg ccggttcggg cgtcgaaggg gctttaactg
     5041 gttccgtggt tgtcggttcg gctggggtgc cggggccttt aacgggttcg gtggtcgccg
     5101 ggggcgtcgg cggttctgcc gtggttccgg gaccctttac gggctctgga gttgtcgcgg
     5161 gcggcttgat cggctccggc gttgtggaag gaggtttcac aggttcggtt gtggccggcg
     5221 gagtttccgg ggctgatgtc ggaggtttca cgggctccgt ggacgggggc ttgaccggtt
     5281 ctggagtcgt gggtggcttc acgggttccg gtggtgtgga aggcggcttt accgactccg
     5341 tggtgccggg cggcttgacc ggctccggag gtgtcgaggg cggtttgacg ggttccgtag
     5401 tggttggcgg cttcacgggc tccggcgtcg atgggggttt gacgggttcc gtggtcgcgg
     5461 gcggtttgac cggttcggga gctgtggtcg gcggctttac ggtttccggc ggtgcggttc
     5521 ccgtcttgcc gggttccggt gtggaagaag gaggcttcgc gggttcgtgc gtcgtcgcgg
     5581 gcggcttgtc gccgccggtt cccgcgcccg ctcccttccc gggttcgggg gggggcgagg
     5641 gcggcttggc gggctccgtg gatgagccac ctgttttcgc cggctcggtc ttcggcggtt
     5701 cggatttggg cgtcgtgttc tcgatcttct tgttggcgtc cgtgtattcc ttcgcggact
     5761 tcttgttgcc cagctcggtc tccttcttgg ccaggtcgtc cctcatccgc ttgttcgact
     5821 ccgactgggc cttcgccgtg tcccgcgcgt tcttgaccgt ctcgtccaac tgcttgatgc
     5881 ggttctgggc cttggcgttc ttgacctctt ccgactcgcc gaaattctgc tcgcccgact
     5941 cgtagggatc ggcgtccacc ttcatctttc ccgacacggg atccaccggc ttgcccgtgg
     6001 cctcctgcat cttggccgcg atcttgttca tcgtccggac atcgcccttg gccttggccg
     6061 cctcgtactt ccggttcagg tcctcgacgt agccctccgg atcgtactgc gcgttgggcg
     6121 acttgatccc ctcgtgctcg agggcgccgg accgcgtgta gccctcgggg ttcttcgcgc
     6181 gccccgccgc agacttcggc gtctcgggct cccagatttt cgtgtcggcg gtatggtcct
     6241 ggtacatccc cgtctcggga tcgtacgtga ccttgtgccc ctgtcccttg aggtggtcgg
     6301 cggcggcctt ggcggcttcg ggggtatccg ccgtgatgtc catgtccgaa tgcgggctcg
     6361 acgggttgct gccgctgctc ttcagcttgc cgccttcggg cgtgttctgg agcgactccc
     6421 aggccttcgc ggcgggatgc ttggcattgt actcgttgcg cagcgcggtt ttctggttga
     6481 cgtagtcctg ctggagcttc ttgtagcccg ggttctcggt gtccaccgtg ccgtccgact
     6541 tctggtattt cttggccgcc gcgtccgtcg cgtcctgcag gttcttgacc ttggcgtcgt
     6601 actcgttgcg caagccgtcg atatccgtgg ggggcggggg aggcggttgg ggcaggaggg
     6661 tccaattact atggttatcc ttgtccttcg cgtcctgcgc gccgggggcc gcccacgacg
     6721 ccaggagcat cagggccatc agcaggatcg gttttcgcat gttcatgctt caccctcccg
     6781 cggctttact gcggcgcccc ctgcaacagc gaaccgacca gcgtctccca ctcgggttcg
     6841 gcctgcttca tccgctcctg caccatttcc atcacgcgcg ccgagatcgc cggggagagc
     6901 gccgcgaact tccggcccgt ccggctctcg taaaaccgga cgatctccgc cagctctttc
     6961 tccgtgagct cacccatgta caactcgatc acggccggct tcagcacctc ccagccgatt
     7021 tgcttggaca ggaaggagcg aatggctgtt tccgagcccg cgaggccggg ctccgtggcg
     7081 accgcctgct tgaccagggc atctatcgcc gactggcaca tggtctccac gctgaagagg
     7141 tccatgagct tctcgaccag ctgacggtgc gcgatcggcc ccgccggcgc ggcgttcgaa
     7201 gcaaggacaa ggatcagggc caaggcggac agtttccacg tcttcatggc aacctccatt
     7261 gaacacgcca cacggcggcc tctctggtac gaattataat gaaccgatcg gccgccgaaa
     7321 acaagtattt tcagcctctt ttggcgtgcg acaggaccca cgacgacgcg acgggggcca
     7381 cgccgctttc gagcgccgtc gcgtgctacg ggacgggcgg cgggtagagg tccggcgagt
     7441 catccggaac gcttgtgggc gtggtctgcg aatcgatgcc cccgggattg tctcctcctt
     7501 cgaccgtggt gtccgtctcc gagccgtcgg tggtatccgt ggtgtcggat gatgtgccgt
     7561 cggaagtccc ggtgttgccg gatgggccgc cgggataatc gtatcctttc ttctcgcagt
     7621 cgtggcacag ccagccgacg ccatcgaact ccatgtacgc cggcccgccg cagatcacgc
     7681 agacggggcc gctgtcgacg gtactggacg acgtcgtggt ctcggtattt gtctcgccgc
     7741 ccacatccgg ctcgtagttg tggccgttgt aacttgtgat attcgtattg ctgcttgaat
     7801 tgaccgtttg cggtccgacg ggcgggtggt ccgtcccggt attggtgcgc cccgtgttgc
     7861 cggtgttggt gcccttattg ttgccgccga agatcgagtc cccggccttg ccgccgaggt
     7921 tctcgcccag gtgacccgcc gcggtcttga ctccggtgtt caccccattg acgacgccgt
     7981 tcaggagtat gttcttggtg ttgttctgtg cgattcggag gttgtgctga ttttcggtcc
     8041 cgatcttcac ggcctcctcg ccgcccgcgt tcagcgttat cttcgcgttc ttgagcgtct
     8101 cgttgccgct gttcgaggcc ttgtcgatcg ccgtgttgtt gcccatcacc tccaggcccg
     8161 tgtcgtattt cttcgccatc ctctcgttct gctgcccgat catgccggac aacacctcgg
     8221 gcctgctgat actgatcgcc tcggtatcct tcaaggactc ggcttcggat ttacgcatat
     8281 ccgcaaccga gagggactga tcggtttcgt cttcagccaa tgtttccgcc tttatcttgc
     8341 cgttgccgag atcccgaatt tcggcttctg acaggatcat ggttcggaat ccgcagttcg
     8401 ggcaaacatc ccgccattga ttgaccttcg acggctctgg gggggaacaa gacaaatatt
     8461 ccggatgctc ttcgaggacc gacgtcattg cttggccgca ttttggacag gcatggctgg
     8521 cgctggccat ggaggtttcc agattgctcc atttatccgg tgtgttgatg gcgttaacag
     8581 tcgtctggtg ttgaatgtcc aggacggtgc cacagttggg gcagacgatt tcagtggtgt
     8641 agcccgcgcg tgatgagtcg tcatccttta ccctcgtaac cgcctccttg aatttgctgc
     8701 cacagtgaag gcaaaacttc cccggctctt ttgcgtcgca tcccgacggg gcggttaaat
     8761 agtcggcttt tgcgcaattt tcacatgccg acaatttgga gttgaagggc aagggagtca
     8821 tttttgatga cgtgactcca tccgtcccct gttcgaatct tttcccgcaa atcgcgcaag
     8881 tcaggaatga cgatgtggca atcgccggca tctggttgcc cgtttgattt ccgccaacct
     8941 ggccgttgtt tccccgattg ttgccgtctt gatttgtatc ggtctggccc tcgcccgtgc
     9001 tggcgggata ggttgtagta ctgatgacat ttccgttgga gtcgtagtcg gtgtgggtga
     9061 cgctgacgac gttgccgttg gaatctgtcg tctcggtcac cttggtggtg cctgtactgt
     9121 ttttagccca gcccttcacg atggtcgtcc ccccaaccgg ggactgaggc tcgttctgct
     9181 cgccggcgtg tattcctcct tggttcccat tattgtcgcc gccctggttg ccggcccaaa
     9241 cattgacgcc gccttcattg tcccccccgg gcggattccc ttggttctgg cccggattgt
     9301 tcccgtcggg ttggttgttg ccccctttgg tgccgtcctt ggtgccgcct cgattattcc
     9361 caccgggatt attgtcgccc tggttgtttc ccgtgccgtt gttgtcgccg ggctggttct
     9421 cgccctggtt gtccccgccg gcgttattcc cgcccgtatt ccagttgttg ttcccccctt
     9481 ggttattgcc ctggttgttg gcgcttccgt tatttccgac cgggacaggc gtgccccccg
     9541 agctgtttcc gccgccgtta ttacctcccg gaacagagcc accgctcctg ttgttcccgg
     9601 tgttgccgcc ggggggagcg gtgccgcctt ggctatggcc ctggttgttt cctgcattgc
     9661 cgccaggaat agcggttccg cccgtgttgt tgccggtggc agtcgtagaa ccgggccgtg
     9721 ttgtggacgt gatggccggc cccttcggcg tcccctggtt gctccccttc tgattttggt
     9781 tggaggattt cgatcctgca tgtgccttct gccatgccac gggatcattg ccgggcttat
     9841 ttactacgtt ttttgagcct gcatatcttg tctttgcctg ttggttgtaa agatcgttct
     9901 tgagaatgga cacgatctgg ccccccgtgt attcccagag tttcttgccg ataaagccac
     9961 ctgtcgttcc gcctgccacg gtgccgaccg gtccgcccga agaacccacg gagcctccca
    10021 agacggtgcc ggccgcggtg gagaaaaact tgcccactcc atcaatcgct gtgagacacg
    10081 cttgccccca tttgccttca tacaattccc ccgccattgt cgaaccgcta tcggtcaggc
    10141 tgattatttt gctgcttttg gcaatgatct tgcagccgct gtcgccagga aactttttgg
    10201 ccgtttcgtt gatcagcttc ctggtggtcc agcctacgtt gctttttaaa tccctattta
    10261 ctggatccaa cttgtccgca attggatcta aaattgcatc caacttgtcc gaactgctgg
    10321 cgaagctttc ggaaagcgga aaaaagagcc ccaagatgag aattgttttt cgagcagata
    10381 ggcccgcgtt catggttgct cctcctccag gacagccgaa ggaagttgcg cttgtatttc
    10441 tttccgctgg gtaaaaacag tgggtacatc gagatacccg actcgctgga attgaaaccc
    10501 tgcgtccgtt ttttgaaaag acagggacag gccgagactg ggctggctgt cgcaggtcat
    10561 gaatacgatg ctctcggtcc catcggtgag gaatccggct ggagagaacg aatcgatctt
    10621 gccttgaagc ggctcgaggt agtcgagggt gcgcgaatca agggattgac gccagggtga
    10681 agtgcaggtg gcgagcccgt ggtctatgtc ctcggcgcag cggtcgattt ccttccgcaa
    10741 ggcttcgtac tccggagcgt tcaggagctt ggccacggcg ccgtaggact gcccaagctg
    10801 tatgcccgcc gcggcggaca aggtctcctc gacctgttcg gaggttgctt ccgcccccgt
    10861 cggggaggga acccagaccg tggatggaag cccttcaaac gcttcgatgg tttgaacggc
    10921 gccccattgc acagccagta caggggttat atgcgcggcc aactggcgcc aaagggggct
    10981 catgctaatg ggcaatttcc cgcctttcct taagtactcc gcgggcacga attgcagtct
    11041 tgatatttcc gtgcggtctt cgcgaaaggt ccagagcgtt aaaagagcgg tatcgctcca
    11101 gggatggaag aaaagcacct tcatgctggc tccttcagaa gggcccaagc tgaacacaga
    11161 ggtgttgaaa aagtaggacc aaagtttatc gctatcactc ttgtcgaagg tattggaatt
    11221 aatgcgtgtc agatccgcgc gggcctcatt gcccatgata tcctcgaagg cctgtgccgg
    11281 tgacgcgaca caggcggact tgaagacggc gacccgtttc agaagcaatc cttcgatttc
    11341 ctggaaggat aagggatcct gtgcgcgatc gtcatgggcc gtcgcgtcct gggcgtttgg
    11401 ccacgcatcg tgctctggtt gttgggcata cgcggaaggc catatcgtgg agttgatggc
    11461 cccgaacatc ggggccacaa gaaaggaaag cgagagaagc atccgccttg atgatggatg
    11521 ttgcatgagt ttttccgcgc atttgtttgt caatccgctt cctcggagga acacggatta
    11581 tggcggcctc cacgagcaga atcatgcgcc ggttgcttgc tgatgcaagc cttttccatt
    11641 cgttttgcgt gcggcgggtc gctgcgctgc ttgccgaacg tcatcgcgcc cggtgcgtgg
    11701 cgactggggc gcgccggtgc actccagggg gcggcccgga tgggcgggac gatagccgtg
    11761 cctggccgat gggcaggggg gagcttttcg cttgcgcggc ggcgggcacg tgatagtctg
    11821 cacggctggt tcaaggcata gcggagggag tgttccgatg aacaagtgga tgatcgtttt
    11881 catggggttg ctgctggcgg cgggttcggc tctggcggcc gaggtggacc tgttcgagca
    11941 ggcgccctgg tccggcggcg tcgggctcga tttcatgcat ttcgagggcg accaagcggt
    12001 cgaggacagc gccggcctgt tcctgaagtt caactacgcc ttctcccccc gctggtcagt
    12061 ggatctggcg gccgacctgt tcccgtcgct ccccaaccgc gaattcacga ccgagcggcc
    12121 ctaccagctg gacgacgaca cgtgggccct gcgcctcggc gcggacgggc tcctgcacct
    12181 gcgcacgatc gaggacctcc gctgggaccc ctacctcgcg gccggcgccg gcctgatgat
    12241 cttcggcgat gacccgagcg gcggccaggt ccagcctgcg ctggacgccg gcggcggcat
    12301 gttctaccac ttcaacgacg agtgggcgct gcgcggcgac ctgcgcgcgc acacgacgac
    12361 gctgaactgg gagttccagg cgctggcctc cgtcggcgtc atgtggcgct ggggcgcccg
    12421 cgtcccgccc gccctgcagg tcagcggcgg cgcgctggac agcgacggcg acggcctgct
    12481 ggacaccgag gagcagcaga tcggcaccga ccccttcgac ccggacacgg acaaggacgg
    12541 cctgaccgat taccaggagg tccgcgagac ccagactgat ccgctcaacc cggattccga
    12601 ctcggacggc ctgaccgacg gcgccgaggt gaacgtctac accaccaatc cgctcgacgc
    12661 cgacacggac gacggcggcg tgcgcgacgg gcacgaggtg atcgaggacg ggaccgaccc
    12721 gctcaacccg aacgacgacc tgcagctctt cacgctctac atcgagttcg actacgacaa
    12781 gtcgatcctc aagccccagt acttcgagaa gctcgacgtg atcgtcaagg ttctgcagcg
    12841 cgaccccggc gccaccgccc gcgtcgaggg gcacgcggac aagcgcaaga cctcgcagca
    12901 cgagtacaac atgaagctgt ccgagcgccg cgccaaggcc gtcgtggact acctggtgaa
    12961 cacgggcggc atcgaccgga cgcgcctcgt ctacaagggc tacggcttca cccgccccgt
    13021 ggccccgaac gacaccgagg agaacatgca gaagaaccgg cgcacggaaa tctacatccg
    13081 tccgtccggc aacgtcccgg cggtgtccac gacgcagccg atggtcgtcc cggcggcgga
    13141 aggggaaacc cccgccccgg ccgaaccggc gaagtaatcg acgccatttc ggatgagcag
    13201 agggcccgcc gcgtcgcggc gggccctttc gtttaccatc gcacatccag ctcgatggaa
    13261 tcctcttcca ccgtggagca ggggcagagt tcgacgtgga agcggatgtt gcggcggagg
    13321 gcgtcgaggg cattgtccgc gctcaagccg tgggcgctga tatccagttc ggggcaggag
    13381 gccacgaatt ctccgtccga cgattcgagc tgggccgtgt acttcatggc ctaaacgtcc
    13441 gccggaaacg gccagagtca atggatggcc gcgcgctacg taccgtgcgg cgcgacaggg
    13501 cggatcaggt gtaacgcagc gacgcctgcc gcgtttccag aaaggcgatc tgccgtttcg
    13561 gccagcccgc ggcgatcgct tccggcgcgg cgccgtcgag cagcgcgcgc cggacggcgt
    13621 cggtccccat gagcttgtcg aagaattccg ggcgcgcgcc gggggcgtcc cagagcgcct
    13681 ccgggccgta cagctcctgc aggatggcga tcagggtgac gccggattgg accggccgga
    13741 acgcggccgg atcggtgatg tccagccgga taccgcggac ctcgagccct ttgagcgccg
    13801 cgccctcggc ccggtaggaa cagggttcga agcggacgcc cggcaggggg tgcctggcga
    13861 gtccgtcggc gagcgccagg gcgtccaacc acggggcgcc gacgacctgg aaagcctggt
    13921 cggtgccgcg gccgtggtcg agcgagggca gggcctcgaa gaacacggtg gccgggaaac
    13981 ccgcggcgca ggcccacgat cggatcgccg gcgagggggg aatccagcgg cccgtcgggc
    14041 tcggcgcgtt gcgtgagaaa ccgcgcatcg gcgcgacgaa caggtcgaga tcgaggcata
    14101 ggtcctcgcg caggaatcgc gccgtctcgc ccggcgtcat gccgtacgag acgggcgttc
    14161 gaacgaatcc cacgaagctc tcgaaggccg gatcgagcat cggcccgtcc acgacgcggg
    14221 gcagggggat cggccggtcg gccacgatga ccgccttccc gttgtccgtg gcggcctcca
    14281 tcacgtagcg cagcgtcgag acgtaggtgt agggccgcgc gccgaggtcg tgcaggtcga
    14341 agacgacggc gtcgagatcg cacagcatct ccggggtcgg cttgcgggtg tcgccgtaga
    14401 gcgaacggat cggcaggccg gagaccggtt cgcgcccgtc ggcgatgact tcccccgcgc
    14461 cgccgcgccc ggtgaagcca tgctcggggc cgaacagggc gacaaggttc accccggcgt
    14521 ccagcatccg ctgcagcgaa tgcgcgccgt tggaatccac gctggccggg tgggcgacga
    14581 gcccgactcg ccggccgcgg agtcggtcca ggtgatgaat cagtagagtc tcaatgccgg
    14641 gttgcatgac aaggctaacg catagacttt gctcggccgc agatcaagcc gttggtaggg
    14701 cggcttcgcc gaagccgccg ttctccggcg cgttgagcca acgcgcccta cctggggtgg
    14761 gaaggccacg cgatgcgttc gccccagaac agcagttcgg aaggaacgcg gacgcgcgcc
    14821 ttgaggtcga tggcgatgtc gtacgaggcc gggaagccgt cgtacacatg ctccaactcc
    14881 gccaggtgtt caaacagcag cgcgcggggc cggaccttgt cgagcgcggc ctggacggcg
    14941 tcgtacgtgc ggccggtctg cgccgcgtgg ccgggctcgt acgtttcgtt cggatgccgc
    15001 cacgggacga agaacaggtc gatatcgcga ccggccgtct tttcaagttt cttgctgacg
    15061 tccaggtcgc cgatgaacag gaacgtgaag tcgcccgggc agacgatctc gtaatagaca
    15121 agaggcaagt cctccatcgt cgcccgccag acgtggaagc cgcgccgcgc ggtgacatcg
    15181 agtccggcga ctttgaatgc caggccgtca tccgccgcga tgacggtctt aaccccgggc
    15241 cagtccgcgg cgaggggggc gggcaggacg acgggcttgc cgcgctcaag gcaggcgcgg
    15301 acgagggcct tgtcgtagtg gtcggggtgc cggtgcgtga tgaacagcac gtccagcagg
    15361 tcggccaggc gcccggtgag gtcggcggaa tccgtccagc cgaaccggcg ggggaaggga
    15421 aagacatcca tcccgatagc caggccgtcc gcggtcagga cgaccccgtc gttgtagagt
    15481 tgccacgctg tcggcggggc gtcggcgggc tcggcctcga ttccgtcgag cgcctggagc
    15541 agccgggtct cgatcgcctg ccagaaggga ttggggcttg ttttcaggtc gaacggcgcg
    15601 ccggcgcggg tgttgttttc gatgaattcg ccgattttca ggaaggcatc gcgccgggcc
    15661 gggctggcgt cgaacgtggg tggattggag tcgagcacca gcagcgtggc cagaaaaaca
    15721 tccaggttca tgggcgcagt accgtgttgt cgatcagtcg cgtcttaccg acgaacaccg
    15781 ccagggcggc catcgccggg cgcgcaacgg tcttgaccgg ctccagcgtg gcgtcgtcca
    15841 cgatctcgat gtaatccacc cgcgcgagcg gggcctccgc gatgacttgc tgcatggccg
    15901 cgcggattga cccggtctcc cgcacaccat cgcgcgtcaa tgcttccgcc cggtccagcg
    15961 cgcggcgcag gcacagggcc tggcggcgct cctcgggtcc gaggtacttg ttgcgcgagc
    16021 tcatggccag gccgtccggc tcgcggacgg tcggcccggc gacgatctcg acggggaagt
    16081 tcaggtcgcg gaccatgcgc cggatcacgc ggagctgctg gccgtccttc tcgccgaaca
    16141 cggcgacgtc ggggagcacg atgttgaaca gcttggccac cacggtagcc acgccgcgga
    16201 agtggccggg ccgcgtcgca ccgcagagcc cgcgggacag cgactcctcg accacgtaga
    16261 cgctgtggcc ggtcaggtag atgtccttcg cctccgggta gaacacgatg tccgtcccgg
    16321 cctcccggca cagggcctcg tcgcgctcga agtcgcgggg gtacttggcc aggtcctcct
    16381 gcggcccgaa ctgggtcggg ttgacgaaga tgctcacgac cacgatatcg ctgcgcgcgc
    16441 gggcgaggcg gacgagggcc atgtgcccgt cgtggagata gcccatcgtg ggcacgaagc
    16501 cgatgcgccg gccctcgcgg cggagggcga gcgaccgggc ctgcatgtct gccggcgatc
    16561 gcacgatttg catatcgcac actctagcgc ctttccgccc gccgctcaat gccgcccgcc
    16621 gacccggagg aataccgggc ccggcgcggc gacctcgccc gtccaggcct cggtgaaaac
    16681 gccggtggcg gacaggcccc agccctcgcc gaccaactgg gtggcggcgt gcagttcgta
    16741 ttccagcgcg gtccgatccc aggccagcgc gacgcggctg gacccggcgt cgggccggat
    16801 cgtggcgagg gtcggcgtcg cgggcgcgag ggcgtcgagg gcgacgctgg cgcccgtgaa
    16861 cagcaccggg ttggtcgtgt cgagccggaa cgtgaccgtc gcggacccgc cgggggcgag
    16921 gatgcggttg tattgcttcg cgtggacatc gaggtgcgcc agggtctggg tccagaaccc
    16981 gtcccacagc cacgagacct ggcccgcgct gatgtccacc gccgtgttcc agtggtagat
    17041 cgtccccgcg gtgtcgttgg tgacgacgaa gtacgcgtag gtgtgcgtcg catcctggga
    17101 gatcacgttg aaggccaccg tgccgccgct ggtcggcgcg aattgccagt acagattggt
    17161 gggggccgga taggccggcg ggcagccccc gatcactcgc aacgtgcggt acccggggta
    17221 cgcgcaggcc gaaagattgg tctgcggcga cacgcgcgcg gtctggaaca ccgccgccgt
    17281 cggcgcggcg ctcacgtcga cgggatcgcg catgtaggcc gcgaggtccc ggtaggcgac
    17341 gaaataaaca tgggggtacg cgagcgtcca ttccacgaag tcattgagcg ccgcgatccg
    17401 ccaggccgag tgcgccgggt tcgacatcca ctggttcgtg gtcgtggcat gcagggcgag
    17461 cgtcaagggc gcgcggttcc cgtcgtagtg ccacaggaaa ttcgtcttcc acaggttcat
    17521 cacggcgttc gagtcgtagc cctcgggcgg atccatgatc gtgacgggtg aaaggttggt
    17581 cgagaacagg tcgtacaagt ggagctcgaa caggcctgga tacggctgcg ctggttggcg
    17641 gacggccggg acgttttcga cggcgccgtc gtcgagggtg taggcccagg tcatggcggc
    17701 gggcgagggg ctcatgccag ccaggtcctc aaagagcgag gattcgtaga ggaagctgcg
    17761 ctccgccagg atgcggaaac tgccgtcgtt gctgaagaga tagggggcgc ggaagccgac
    17821 gatggccgcc tcggggatac cgcccagcgc ggagagcgtc gaccggcagc cgacgatctc
    17881 ccgccgccag gtgtccggca gcgtgttggt cccggtggta tgcgtcatgg tgtggacggc
    17941 gatctcgtgc ccgtcggcga acagccgctg cgcgagcgag taatcgatcc gggagtccag
    18001 cgacacgaag aacgtggcct tgatcggctg gccgttggga ttgacgtggt tggtcaggac
    18061 gctctgcacc agcgcatagg agtccgtcgt gaccgagtcg tcccacgaca gcaggatcat
    18121 ctggggcgtg ttcaggggcg ccagtccgcc cggcgggttc gtggaggccg cgatgcagtc
    18181 cgggggcagg caatcgccct gcgcgcgggc ggcgagccac gcggccagaa gaacgaacgt
    18241 tgccacttgc ttcattcgta cgactgctcc ggccccggga aggcgccgga ctggacttcc
    18301 tgcctgtact gcgcgagggc ctgcgcgacc tgcgcgccga ggtccgcgta gcgcttgacg
    18361 aacttcggct tgaagtcgcc gtacaggccg agcacgtcgt gcagcacgag cacctgcccg
    18421 tcgcaggccg gtcccgcgcc gatgccgatc gtcgggatct tcacggtggc ggtgatctcg
    18481 gccgcgaggt cgctgggcat ggcctcgagc acgagcgcga acgcgccggc ctcctcgacc
    18541 gcgcgggcgt cttcgagcag ttgcttcgcg ccctcgtggt tgcggccctg gaccttgtag
    18601 ccgcccatga cctcgatgct ctgcggggtg agcccgatgt gccccagcac ggggatcccg
    18661 ttctgcacga gcgcgtggat cagcggctcg cggaacgcgc cgccctcgat cttcaccgcg
    18721 ttggcgccgc cttcctggat gaaccgcccg gcgttgagaa tggcctgctc gatggaggcg
    18781 tggtaggaga ggaacggcat gtcgccgacg accagcgccg tcttcacgcc gcgcgcgacg
    18841 gccgccgtgt ggtggatcat gtcgtccatc gtcaccggca gcgtgttctc gtagcccagc
    18901 atcgtcatgc ccagcgagtc gccgaccagg accaggtgga tcccggcggc gtccacgagc
    18961 cgggccatgg cgtagtcgta ggccgtgagg caaacgatct tttctttccc tttcagggcc
    19021 ttgatcttcg gcgcggtcca gggggatgta ttcacgaggc tatcctacca ctccttggcg
    19081 aaaagcgcga ccctgggctc ggccggcagt tcggcgagca cctgccggac gggccgggtt
    19141 tcgccgggaa tgaccaggtc cggccggatc tccgccaggg gctgcacgac gaagcggcgc
    19201 tccttccagt gcggatgggg gatcgccagg ccgccgctct gcaggctggt gctcccgacg
    19261 cagatgatgt ccaggtcgat cgtccgcggc gcaaagcggt cgagcccgcg cttacgtccc
    19321 agcttggcct cgagcgcgtg caggcgctgg taccacgcat gcccgtccca ggggctctcc
    19381 aggaccagca cggcgttgag gaagggaagg tggctgtact tttccagcac gccgaccggc
    19441 tcggtttcat agaccggcga ctgggccgcg atgtggacgt ccggccactc cgcgatccgg
    19501 cggcgggcca tcgtgagggc cgccagccgg tcgcccaggt tcgagcccat gctgattatg
    19561 gtttccatga tcggcgcatc cggcggatac cggcatgaag ccacattgcc gcgccgaacg
    19621 caagccccag ccccgtggcc gcgtcccggt agcgcggggt atgggccacc catcggtcgg
    19681 cccggaaccg gtccggagcc aggaacacgc cgcccgcggt gaccagggcc tgccggtcca
    19741 gcgaaatgcc ctccacgcgc aggacctccc ccgagaacag gcgcaggcgg cccccgtcct
    19801 ccggcaggaa cacggcgcgg tcgaacgccg ccgcccggcc cggccggcgc tcgacatcct
    19861 tcagggtgcg gatcgagaac gcgtcggcct ccagtggtcc ctcgatgaac gccagcggca
    19921 gcagtccgta cgcggcgagc cccgcggccg ccgcgaggag gaggcggctg cggaggggcg
    19981 ggatcgggcc ggccggcgcg cgaacctgct gatagaggcc ccacaacccg acggccacgc
    20041 cgagcgccgt cagcagcgtg accgcccagc cctccgccgg cagcaggtcc cagtccgtca
    20101 tccgccagct cgcgggcgcg aacaggaaca cgccgttgcc gcccttctgc tccgccgcgt
    20161 cgagtagcag gtgcaacagg gagttgaacg ccagcgcggc ccaggttttc cagaacgagg
    20221 aggaaaggag ggccagcgcc gcgccgagca gaaggcagaa cgccagcgac gactggacta
    20281 cgccgtagag cttcagatcg tacggattcg gggtgaaggg cagaacccgg atcgcccgct
    20341 ggagaatcca cggcacgtcg gggatgacca ggcccaggaa gacccacttc aggtcggcct
    20401 tccggaagac cgccttcgac acgatcccct gcgcgcccag atgaaccaac gtgctcggca
    20461 tatcgtcaga tcctgcgccg gttcgctatc cgtcagaacg tcgtgacttc cgccgatcgg
    20521 atgaacggct ccaattcagc ctcccactcc ggcccgccca gccgccgcgc gaactcgacg
    20581 gcgaggtgcc ggggctcgat gccccgcggc cggttccggc cgaggccctg cagacaggag
    20641 gggcagttgg tgaggaccag tttggtgccg gccttttccg acgcggcctg gagcgcgtcc
    20701 gccttccgct ggagcatgga gctggaaata tccggccggc tcaaggccag cgtgccggcc
    20761 tcggagcagc agtggggaac gggttcgacc ggccccgcgc cgccgcgcgc cagcagcgcc
    20821 gggccgctgc ccgcgagcga gtcgtggcag ggggcgtggt aaagcgccgg cgcgccgccg
    20881 ccggggattt ccgctccgcc ggcgaggacg aattccgaca cgtcgcgcag cggacagccg
    20941 accacggcct ccagccccat cgcgcgcagg gcctccatgc acgtgccgca ggtcgcgaca
    21001 caagcgtcga aacggaggta gccgaacatg tcgcggatct ggctgaacac gatcgtgtcg
    21061 tgcagcacga tccggttatg ccgctcgccg tcggcgttcg cgtgcgccgg gaacccgcag
    21121 cacaggaacg gcggcgggag gacgacctgg accccggttt tgaccagcag gtagagcgcc
    21181 gccagggaga catcggagaa caggcgttcc gagccgcagc ccgggaagta gaagacggag
    21241 gctcgcgccg cgccctccgg ccggaagcag agcgtctggt tgtcgcggca gggcgggagc
    21301 cggtcgcgca gggtccccgg cggcgccggc ggcatgggcg aatccagcag cgcgtggccg
    21361 ggcacacgtt ggatcaggga tttcggcagg ccggacgcca cgcggtgagc gagccgctgg
    21421 caggccccgc ccgcgcgcag cacgccggcc cggaacaggc ggttgaacag cgggcggcgg
    21481 ctctcgaggt aggccagcgt caggcgcgtc ggcgccgggg tatgcttgaa atgccgcgtc
    21541 ttgagaatgt tccgctccag gatggagacc tcgccggtgt cgatgtccac ggggcacggc
    21601 ttgaggcact tgtggcaggc cgtgcagtgg tccgcgatgt cctccaggaa gcgcaggctt
    21661 tcgaaccggg tcgagcgcga gcgttgcgcg tcgtagagca gcgcctcgat cagcgcgccg
    21721 atggccaggt tcttgttgcg cgggtgatag aacaggttcc gggcgggata aaacacgcag
    21781 cagtccgcct tgcatttccc gcaccgcacg cacttcgcga tcttctgcgc cagctcctcc
    21841 agcgagccgt gctggaggat ccgcgcctcg agttccagga ggttgaacga cggggtgaac
    21901 accgcatcga gcgcgccggc atcctgcagc ttgccggggt tcaggaggcc gtccgggtcc
    21961 aggcgtcggc ggtgttcgtc caactcgcgg atgcgctcgg gctcgaggtg cttgagcttg
    22021 gtgatgccga tgccgtgctc gccgctgacc gcgccgccca tcgccaccgc cgtccgcatc
    22081 gccccgtccg cctcgcgcgc ggcccgctcc atcatgtcgc ggtcgttcga gaagaccggg
    22141 atgttcacgt ggacgttgcc gtccccggcg tgcatgtggg tggcgaggac gatgcgccgg
    22201 gagcgcacgt ggcgctcggc cgcgtccaag tccgcctcga tctgtcggta gccggaaacc
    22261 aactgcccga gcccggcccg cagctcccgg atgatccgtt cgctccgcag gtcctcggcc
    22321 ggggcccgcg cgagctgctc cgccgcctcc gcgcacaact gccgcgcccg cgggatcttg
    22381 cccgaatacc tttctgcgtc ctcggcgacg gcggcggact cgacgcgccg cccgaactcg
    22441 gccgtcagcc actgctggtt gcgccggttc tcgtcgttgt tgcgctcgtc cacgtagcgc
    22501 gcgaaccggg ccagcgcttc aaggggtagg acgatgtctt cgttgagttt gaacgcgttc
    22561 gtatgggcgg cgatggcgcc cagcttcttg cggtcctgcc agaaccgggc ggcctccgcg
    22621 tcgtcgcgcg ccgcgaacag gcacgtgttg gggtagggcc ggagcaacgc ctccaatttc
    22681 cgcgtcccgt ccgccagttg ctccggcgta tggccgacga ggtcgacgag caggaccgcc
    22741 ttcggcggat gcgcgcgcgg ggccttgttc ttgtagccga tcgcgcgcac gtactggtcg
    22801 tcgaaatgct ccagcgccat cagcgcctcg ccgcccgccg cctcgaacgc gcgcgagatg
    22861 tccacgatca cgcggctggc ttcgtccatg tcgtcgccgt aaaactcgag gcagaaggtc
    22921 cgccgctgcg ggtacggctc gtggaggata aactcggccg acgtgatgag gccgtcggtg
    22981 ccttccttct gcaggccggg caggccgccc agcgccttgt tcgtcacgtc tttccacaag
    23041 cccggcttgc ggatgtccga gccccgcagc gcgatggtcc gcaggatccg gccgtccgct
    23101 ccggcgacgt cgaattccac ggtgtcgtcg ggccggatct tccggccggg atgatcccgc
    23161 cgccggacct ccaggttcgt cgcgtccggc aaggccatac ggtagcggag cacgttgtcg
    23221 atggccgtgc cccagagtac ggcggtcttg ccgccggcgt tttcggcgag attgccgccg
    23281 atcgtggacg cccaggaact ggtcggatcc gtggcgaaga ccttgccgcg ccgccgggcg
    23341 tactcgatcg cgtcgtcggt gatcacgccc gcctcgacgg cgagcgcatt cccggcgatg
    23401 ccgcggatgc ggttgagctt ctccgtgttg atcatcacac agccctcgcg gaggggcacg
    23461 gccccgccgg tcaggccggt gcccgcgccg cgcgggatcg cgtgcaggcc gaggccgcgg
    23521 atggccgcga gcagcggggc gacctcttcc tcccggcccg gccgcgcgac cgcggcgggc
    23581 aggcagagcc gccagtccgt ggcgtcggtc gcgtggcaca cgagggcgag ggggtcgaac
    23641 aggacgtttt cctcgccgat gatcgcgccg agcctgcggc gtatttgcgc gcggcgggcg
    23701 ggcgcggcgg cgagcgcggt ggccagttcg tccgccaccc gctcgcactg gtccacgatc
    23761 cgcagcacat cctcgttagg gccggcgccc gcgcggacgg ttttcagttc gctccgcatc
    23821 tcgtcgaagt aggcggcctg gcgccggggg tgatcgagca agtcctggac catgaaagga
    23881 ttgcgcttca ggacgaagag gtcgccgatg acgcgcagga gcagccgggc ggaccggccg
    23941 gtgacgcgct tggcgcgcag gccctcccac cgggcccacg tttccgcccc gaacaggaat
    24001 cggatcacct gcccgtcgtc cgccgaggta tagttgtacg ggatctcgcg cacggggcta
    24061 tcaagccgga agagaggccg gccctcaaga agaaaccgcc ctgtgcgtaa cgaaacgctt
    24121 cacggatgtg aagcaacatg ttgcggttac gcgcggcgcc gtttccggcg gccggcggcg
    24181 cgcaggatgt cctcgtacca gcgctcgaac tggtccgtga tctgatccca ggagaactgc
    24241 tcgcggacgc gggccgcggc ccgcgcgccg tactcttcgg ccgcggaggg atcggtgagc
    24301 aacgcccgca gccggtcggc caggtctccg gcgtcgcccc ggcgaaacgc cgcgccggcg
    24361 tcgcccagcg cctccatgtt ctccgggata tcgctgacca gacagcatgt gccgtagccc
    24421 atggcctcca gcaaggcttg tgacatgccc tcgaggtcgg agggcagaac gaagacgcgg
    24481 gcatggccga cgagttcctc gaggaggcgg ccctgcacga agcccggcca gacgatccgg
    24541 ggatcgttgc cggcccggcg ccgcagttcc tcgcggtagg cttcggcgcc gcggacatcg
    24601 cccgcgatca ccagccggtc cgccgtgtcc aggcggcgaa acgcctcgac gaggtagtgc
    24661 gcgcccttgt ccggcaccag ccgggaggcg aacagtacgt agcggcgggg ctcgaggccg
    24721 agcgcgagga tctcgcgggg ctccggcgcg gttttcactt ccgcgccgcc cgggatccag
    24781 cgcacctcga tgccgtacgc gtcccggaag taggcgcact gggtctgcga ggcggccgtg
    24841 aagaggcggt tgcagcgcac ggtcgaccgc tccaggccct tgaggacggc cgagccgaat
    24901 cgcgaccacc gcgcgcgccg ccattcgatg ccgtggaact gcaccacggc gggtatccgg
    24961 cggaggcgcg gcagccaacc catggcgccg ggcccaatgg attgcaggtg gaggatgtcg
    25021 gcccaggggg acagtgaggc gtggagcacc gaggccaggc cggcgctcaa cttctcgcac
    25081 gcggtgagcg gaagggaggg cacgcccacg atccgcatgc ccttatattc aggaacgagc
    25141 gcgccgtaat ggcgcgtcga gtagacgcgg acggcgtgcc cgcgacgcgc caggcgcgag
    25201 ccgagttctt cggtgtagcg ctcgattccg ccgccccggg cgacgtgttt aaccacagcg
    25261 aaggcaatcc tcatatatcg ccagtagttg tttcctgtga cgatcgggcg tgaactcctc
    25321 ctcggcgcgc cggcgggcgg ccgcgcccag ggcgccccac gaggcgcggt tttgccagag
    25381 gctggccagc gcctggcgca gcgcctcgac gtcgccgggc gggaacagga ggccggtctt
    25441 gcccggctcg accaactcgg ggatgccgcc catggcggcg ccgatgaccg gcttgccgtg
    25501 ggcgaacgcc tccagcacga ccatgggggc gttttcgtac cattccgacg ggatcaccag
    25561 gaacgcggcg tcccggtatt cgcgggccaa gtcgccgccc gcccggtatc cggcgaagac
    25621 cacctgaccg cgccgcccgt ttcggacggc ctggtcctcc aggccgccgc gcagggggcc
    25681 ttcgccgacg atgcgcaggg gaatacccag gccctccgtc gcgcgcagaa gcgtggtcag
    25741 tcccttctcc agcgacaggc ggccgacgaa caacgcatat gatccctctc caaaggaagg
    25801 gcggatcgag tcggtggcga catggttcgg cacatacgcc actcgttcgg gagcacgacc
    25861 gttgtccagg aacgtctggc gcaggaagcg gctcggacaa atgaagcgcc gaacgacggc
    25921 gtatttccgg ccccaggcgg cgaggtaggc ctccgcggtg tacagcgccg aggccgcgag
    25981 gctggacttg tggcatcgtt tcagcaggca gcggtagaaa cggccgccct tgcaggccgt
    26041 gcacacgcgc ccgtgatcca gggcgaggta ggacgggcag acgagcttgt agtcgtgcgc
    26101 ggtcaggacc acgggcaggc ccgcgtcccg ggccgcgtcg agcacggcgg tggtcagccc
    26161 gccgtaaata ttgtgggcat ggaggatctc gggccgcacg gcgtccagga accgccggaa
    26221 ggcgcgcccg accgcgggat tgtacaccac gcggaacgcg tgccgccact tgccgagtcc
    26281 ttggagccgc tcgatgacga cgcgcggcgg gtagaacgcg gcgtgttcgc gcgcgaggtc
    26341 gtccggtccg cggcagccga agaagctgac ctcgtgcccg gcctgccgca tcagggcggc
    26401 ctcggtgaac atgacgcgct ccgcgccgct gcggagatag aggtggttgt tgaggtagca
    26461 gatcttcatg agaagaccct ccccggcggg gcggggatcg gcggcggaac gattgcgcgc
    26521 cgcgcgctga ccaggaacag ggcaaacagg ctcatcacga ggaagttgtc gaggttgttc
    26581 tcggtgatgg cgtaggcggc catcatcgcg gccggcccgc gggcggaggg ctcggcgcgc
    26641 cggaaaagga taagccacgc ggccaggttg gccagcaggc cgagcacgcc cacctcaaac
    26701 agcaggcgga ggtagtcgtt gtgaggcagt ttccccagga agaccgggct ggaaccgatc
    26761 ccgtggccga ggagccggga gagaacgccg gaccggcgga acttccacag gagatcgtgc
    26821 caatgcgcca ggcgccagat cgaggaggtg tattccacgt tgccgtactc cgtgcccagt
    26881 tcccagaagt tgaggtcgct gcctgccgcg atgtcggcgt agtgctgctg cgcgatgtac
    26941 accttcgacc agaggggccc gacgatccgc atctcggcga gagccgccgg gtcgatggtg
    27001 tgcaaggtgg ccagcaggag cgcgcccagc agggcggccc cggccagcgc ggcacaggtg
    27061 agcctggcga aacgcgtgca cagcagcgcg acggccaggc cggccatgta gcccatcagg
    27121 gcgcccgtgg tgccggagag aatgaccagg gcgagcacga gcgcatagag gccgatgatg
    27181 aaccaccgtg cgtcccgcac gcgatcgacg aagaacagga ggctcaacgc gaccagtgcg
    27241 tagttgttgg gattcgcgaa cgagccctcc acgcggtcct ctcccgagcc ccgcgcgagg
    27301 ccgatcaaca ggatggacag gatggccacg agcccccacc agcgctctgg cgtccggtag
    27361 agggggggga gtcgaagcga cgaggccgcc aggaggacga acagcaggga gaagtacttg
    27421 atggtgtagg cgggggcgcc ttcatttccg gcggtcaggc cggagagcag ggcgaccatg
    27481 ggcagcaggg ccgagaacaa cagggccacg gtcaagccgg tcaggggcac gggcagaagc
    27541 cgccgggcgg agcaggccgc aacgacggaa aggaagagaa cggcgcccac catctgcctc
    27601 tcgccgccgg tcgcttcgtt cagaaacgtg aacaacacgt agagacaata gctcgccatg
    27661 agaatgcgcc aaggggaccc gcttcccggc gggcgcgcgg ggacggggag ggcctggggc
    27721 gttcccggag gcatgcggtc agccgccccc gttttcctcg gcgggcgcgt aggccttgta
    27781 atgctgctgg taatggtagt aagggctgta ggtgttgtag agcatcttgg agacatcgag
    27841 gtcgttcacg acgagcccca gcgagtgaat gccggactcc cgcatgcggt gaatcgtgtg
    27901 ccaggtcagc cgcttccggc tgacttccgg ccgcaccatc accagcgtga aatcggccag
    27961 cggcgccagg gccagggtat cgctcacgag cccgagcggc ggggcgtcga gcaccacgtg
    28021 gtcgtacttc gtccgcgccc acgaaatcag ggcgcctgcc agcgcgccca gcgccaccgc
    28081 cgggttactc tggctcatcg gccggctggc gatgacctcc aggttcgggc agtcggccac
    28141 ggggtagggc aagcccgcca tgcgggggtt cgcctcgggc tgcacggtga gcaggtcggg
    28201 ccgtccgggc gggatgggaa agatgccggc cagccggggg cggcgcaggt cgaagtccac
    28261 gagcaggacg cgccgtccct tcttcgccag gatcgccgcc aggttgcagc tggccacggt
    28321 cttgccctcg ccggggatgg aactggcgat caggatcacc tgcgaccgct ccttgtgctg
    28381 gggcgaatcc agcatggtgc ccagccccgc gaaggcctcg acgacctcgc tgaaatgctg
    28441 ccggatcgtc gcggtcgcga tggacgcccg gtcggcgttc cggacgtgcg gcacgacggc
    28501 caggatcgga atcccgcggc cctccatgtc ctccgggccg gtcacccggt cctccaggaa
    28561 atcgagggcg acgatcaagc ccaggccgcc cacgaagccc agcagcaggg ccagcgccag
    28621 cgtgtggagc gggttgggat gcacgggctt gagggggacg gtagcccggt cgatgatctt
    28681 gaccgtcgcc gtgttttcgt cggccgccat gcgcgcgtcc tggatgcggg cgaggatgcc
    28741 ccggaaggac tggtcggccg cgtcccggga gcgttccagc gcggcgagcc gcgtcttgcg
    28801 ctccaccacc tgcatctcca gcttggccgc cagctggatc tgctcgtcct tcttccggcg
    28861 cagcgtctcc gcctgctccc ggaggagatt gttgttcgag cgcgccgtgg tcttggcccg
    28921 ctccagggcc ttcaaggcct cctcgcggta gagcgccacc acgttgtctt tcgcctgcac
    28981 ctcggggtgc ttgggcgtga acgcggccag gaggctgttg cgctccacga cggcgacgcg
    29041 ccactgctcc agggccgacc ggatttcctc ggcccgggga atatccgccg ggaattcgcc
    29101 ggcccgttca ggatcgaggg tgagctgttc cagcttggcc agcagggcgc tttcctgggc
    29161 ctcgcggctt tccacgtcga cgagggactt gttgaatccc tgcagggcgt cgtccaccgt
    29221 cttgcgctcg ctttccagcg cgtcgatctt gtgttcctgc cggaaggcca gcagcgcgtc
    29281 ctccgccttg agcaaatcct tgcgctgcag ttccgcctgg gcttccagcc aggcgacggc
    29341 ggcgtcggag gtcgtgcggt tttcctcgta ggcgctggct tccgcggcgt cggcgaaggc
    29401 gttgcaggcg gccgtcgcga ccttcgggtc gggatgctcg aactccacgc ggatcaggcg
    29461 ggagcggcgg accagcgtga gcgtcagggc tttctcaaag cgcttgagcc gcttgtcgtg
    29521 ccggagttcc gggctgtccg aggaggcggg cgaggccggc gcgaaggcgc cgggaaggac
    29581 ggcgtccagt cgctggaggg cggcctcgag catggtgcgc cccttgaact tctcgaggcg
    29641 cgtgttgaag acctccgtgg actgctgggc gccggcctgg tcctcgatca ccgcgggctg
    29701 ctgcgtgagg atgcgggggc gccgcacgct caactcgata aggctgttcg cccggtagat
    29761 cttctcggcg gcccacaggt agccgagcgc cgcgaccacg gcaaaggcca gcgtcagcgt
    29821 cagggtgcgc cacttgcgca gcaggcgccg ggggtccagc agggccagtc cggccggggc
    29881 ggcgccgtag agatggtcat agtagagcga cgccccatac agcgacgccg gtttctgctc
    29941 gggggatggt tctgtgctca tggttgttgc cgcatttcaa tgtttgaccg ccgccgcgcc
    30001 gacctcccgg ggctcccgcg ccgtgcattt cggaagtgcg gccaacacaa ccgtccaggt
    30061 ccacaggacg gatggacaac ggaacggcaa gtcgattaga ctgaacgccg cgaccaaggt
    30121 caagccggcg catgacaggg tgaagagggc gccgcgggcc agatcccgcc ggaacaggcc
    30181 cgccgccagc gcgccccacg ccagcgccag tccacccgcg cccgcgatcc cgaattcgac
    30241 caggaactgc agggggtcgc agtgcgcgtt cgcccggccg ggattgcttt tcagggagga
    30301 ccagtggtcg cgcggcaggt agaaggcggc caggtaccgg aatccccagc cgcccgtccc
    30361 gtaccagggg tggtcccgaa agatggccca ggcggcgccc cagagccgcg ggcggtccga
    30421 gaggtccagg ttgacctgtt ccagtcccgg cacggcctgg tgcgccggac ggcgccggag
    30481 cgtgaactcc cttcggatgg cctcgccgcc cgccccggcg acggtcagga cgaacacgac
    30541 ggccgccgcg gccgtgagca cggccatccg gagccgggcg gccgggcgcc acaaccgcca
    30601 gccgcgcgcc aggccgtagc cggcgataaa gagcgccagc gcccacgaca ggatgatccc
    30661 ggcgcggctc aacgagaggt tggcgccgac gaggcagagg aggaggctgg cggccagcgc
    30721 ggccttttcg atccgccggc ccggccggtc gcggcggaac acctgccgga acagcaggcc
    30781 ggcggccagg gccgccatga gcaggaaata ggcggccgca tggttggtgt aggtgaacgt
    30841 ggcgaagaaa tcggcgtatg ccggccggta tccgaaaagg ctttgcgcgc cggccaactc
    30901 ccggacggtc ccgaacaggg ccagtgccgc cgcgttatac gcgagcccgt gcaggaccgg
    30961 gcgcagcccg cgccgggaga gggcggggca tcggacgcac aggcccgcgg cccacgcggg
    31021 gaagaaccag tgcagcatct gcgcggcctc gggccggctg aacgcccagg gccagcgggg
    31081 atgcggcgga tccgtgtacg cccagcgccg gatcgcgtcg tcgaaccgca gttcccgccc
    31141 ggcgttccac cgctggcacg ccaggtaggc caggaacacg aggccgaacc agaagaaggg
    31201 atcccggctc caccagctgc gcggccgggc aacgagggcc gccgcgagca gcagggccgc
    31261 cgccgcggcg aagaacggac cgtccgcggc gcgcaacccg gcccagcgcc acgcggcgac
    31321 ggagaggaaa agaagaagtc cccacgccag gcacttttca cggccgtccc gggtttccgt
    31381 gcaaccgggc ggcgtcggcg cggggactgt cctcgtactc actctatgcc gtcagggaag
    31441 gttctggccc tcatagccgg gcgccggcgg gggcggttcc tcttcttcct cttcctcctg
    31501 cgtcgtggtg gtcgtcggcg tggtccgggt gcgcgtccgg gtgcgtgtcc gggtggtcgt
    31561 ggtggcggcg ggcgttgtcg ccgccggctc ctcgatcacc ggttccggct cggggggttg
    31621 aggatgcggc acggggggca gcgctcctcc gctggcgagg aacagctggt cgaaggacga
    31681 aaccatgccg ccgccctcgc tgccgagagc gtcccgcatg gcggtcaccg cgccggccga
    31741 gcccgagatg acggcgctgt tcgccacggc cttgccgagc cccgcggcaa acacgtcgaa
    31801 ttcctccgaa ggcagggcct tgaacgcgat gcggatcacg tcggccagcc gggccgcctg
    31861 ctcctccggg gccaggttga ggccgatgat cttctcggcc acgctcttca ccacgtccac
    31921 cacctggtcc gggctggcct cttccagcag cgccgcgagc ttggagcccg gcgccgcggc
    31981 ggctgcgttc agctgctcgt ccgtcggcgg agtgaaggcc gcggaggcgg ccagcgtcgc
    32041 gaccccgaga gccagggccg caagcccgat tgctgtcctc gtcttcatga tctgtttctc
    32101 ctttcctgcc gtcagtaccc gatatccacg cccaggttga cccggcccac gtcgtagtcg
    32161 cgtatcgtcg agtcgaccgt ttcgtacatc agttcgccga agaaccggag gtacttcgcc
    32221 gggatcaggt aatccgcgcg gagcttgacc gtgacgcctt cgtcctcgcg gtccaccagt
    32281 tcgccctcgt ccatgaccgg gtcggcgtag tcgtcgatgc ggtagacgcc gctggccgac
    32341 agcccgatgg acggggtcat gcggtaggcg acgcccagcc ggaacacgga gaagtccacg
    32401 atgttggcca ggtggatgga cgacatctga atcccgttgc ggccctcgag ctccacggag
    32461 gtcttgtccg tcgccgccca ggtggccacg gcgtcgaaat tgagcccgtc ctcggtgccc
    32521 gcgtcttccg ggcgatcgag ttgctggaag cccgcgccgg ccttgaggga catgcggtcg
    32581 gtcccgcggg tccgcacgcc cagccgcgcg gcgaagtatt ccgcttcgcc gtccacgtcg
    32641 tcgctctcct gcaggccgcc gacgagcgtg agcaggcccg cggtcttgtc cgtgacccgg
    32701 tgggcggcct ccatctgccc gccgtggccg gacaggtcga agagatcgtc gctgcggtag
    32761 tcggtctggt tgtagcgata gcccgcgctc acgtcggact tgtcggtcag ctgggcggtg
    32821 aggacggtcc cggcctccag gacttcccgc cggctgcgca cgtcggcgtc gaggaaggcg
    32881 tcgggcgaca tgcccccgat gtccgcggcc gacccgtagg tgtccaggtc ctcgaccctc
    32941 cgccaggact cctgggcctc gagggtgatc ttttccgggt tgcctatgcg cagcgtcagg
    33001 gcctcgccgc cggcccagaa atccaggccg tcctcgtcgg cgtacgagcg ctggaccacg
    33061 aagcccaggc cgctgatctg gacctcacgg acctggcagt ccagccgcag ccccacgtcc
    33121 gactcgacga agatatcgtc ctgctcgtcg gacgacgtgc ggccggtgtt ggaatcatac
    33181 gagccggcga cgccggcaaa cggggaaagc gtcagcggtc ccgcgtggaa ccccggctgc
    33241 acctgtcccc aacccgtggc ggccagcagg ccgatcgcca ggaccaagcc catccatttc
    33301 gtcttcatcg gcaccccctt gttagaatac caattcaggc acgtatacca catcgtccgg
    33361 gagcaatacg atatcgtttt cgacgcgccc gcgcacgccg acctcgcgga ggttgatatg
    33421 gcgcgtggtg gtgtggccgt ccggcgcgcg ccgcgtgacc aggatggccg tgtccttggc
    33481 gctggtgtcg aatccgcccg cctgctggat cgccacgctc acggtcaggt cgcgggtggg
    33541 ggggatgctg acccggcccg ggcgcttgac ccgccccagc accgtgatcg aaccccaggg
    33601 cgaaatgctc tccggctggt cgtcccggac aaattccacc atgacctgcg ggtcgacata
    33661 gtattcccgg tattcggcgg tcagccgggc cgtcagggag tccagggtct cgccgcccac
    33721 ggcgagcgaa cccaggagcg gcagcgtcag ggtgctcgtc tcggaaaccc gccgcccggc
    33781 ctcctcgatc tgcttcttcc cggccacgat gacggtgacg ttgaccatca agccgggacg
    33841 caacgtcgcc tgctccgcgc cgttgggctt cgggagggcc gtcgaatgag ccggggccgg
    33901 agcggccgga ggcggcttga cggcgcgcgc ggccttggcc gccctgcgcg atgcgcagcc
    33961 ggtggccatg gcgagcagca cgccgccggc cagggccagc acaagcgagc gcgtccgcca
    34021 ttctttccct gcttgcagca tccggtttgc tctgaacgta ttgccccgtc tggcgttgtg
    34081 actatccacg ccgtccgcac acccatccat tgcgattgaa cccgccggac ggattgtaat
    34141 caagcatttt ccgcgtttta cggaacccat ccgccctctt ccaggcctcg ggcgatggcc
    34201 cccgccacca tcgcataccc cgtgggcgtg cagtggccat ccttcggata ataaagcggc
    34261 ggcgcggatg gagccgcccg catggccggc cgcgtgtcca cgaagcccgc gccgttcgcc
    34321 tccgcccacg ccgccagccg gcgcgccggg taatcgtgcg catccgtcca cggccccttg
    34381 cgggggacgt gcgcgatcac cagccgggcg ccgatcgatc cggccagggc cgccatccgg
    34441 gcgaactcgt cctcgatccg ccgccagtca gcctcgtgcg cgccgccggc ctcgtacacc
    34501 gcggcccagt tttccacgcg gtcgttccgc caggccatga atctcgccag gacgagccgg
    34561 cacaggtggg atcgcttgta tagaaagcgg ccgaccgcgc ccggcgacgc ggcctgccgc
    34621 gtaaggaggt agcccgacgg gtccaccgtg acggcatcca gcccgtagaa ggtgtcgatc
    34681 acgtcgttgg gcacgaagcc gacgatcacc aggtccggct ggaactgcag gccgagcttt
    34741 tccagcagga tgcgctccgg ttccgggaag taccgcggga tgccggcctt gatgatctcg
    34801 acgcgcgggt gatcccccgg ccgggtgttg agcatttgct ccaggcggtg caggtaggtc
    34861 tcctcgaacg ccgcgcccac cccgtacgtg aagctatcgc ccagcccgag aatccggaac
    34921 acgccggggg gcttcgaaag ctcgtgttcg ccgtcgcgaa gcccgaacgc gttgtgccgg
    34981 tagtcatact cgaattcgcc cttgaacgac cggccgctcg cgcggctgtg cggcttcggg
    35041 ccgtacggca tggcgggcgt ggaggcgtaa ttcttgaact gctccggcaa tggcggggcc
    35101 agcccgagcc ggcggacggc aaactcgccg atcgcgccgg ccgcgaccag ggcgatgaga
    35161 agggtcagaa gggccgggag aatcctcttc ttcatgaagc ccgtttccct ccatgcaggc
    35221 ggtcaatcat ccaccacagc gcgcgccggc ggccgatcgc gcgctcggtg tagagtatca
    35281 aggtggcccg ccacgcggcc agtgtatccc gaacgcaggg ccgcgccgcc ggctcgtcat
    35341 cgcgcggtac gggcaccccc aaccgacgga atagctttcg ccttgcgggc acgtcccttt
    35401 tcttgcgccg cagatgactc gcctgggcct tcagcacccg cgcgcggtcg atcggcagca
    35461 gccggatcgc gcccccggcg gcggcttctt ccagcaggcc ccggccccgg cggccgcggg
    35521 cgacgacggc gccggtgccc gcccggtggt ccgggaattc cgggacgcgc atctcgccga
    35581 aggcgaggtc cgccaactcc gcgaaatggt ccgggcacaa cgcgcaccgg tgggggatga
    35641 agaaggaccg gagggcggga tagtactcga cgtactcgcg ggttcgagtc cggccgtccg
    35701 cgcacgtgat cctcatggcg ccaaggccgt ccccgcggcg gtagtccagc gagacaacgg
    35761 aggccggggc gatccccatt cggtcgagca cgtactcggt cgcgtcgaag gtccgcgtcc
    35821 cgctgcaaca caggccgaac gcccagggga tccgctctcg gaaccggggc cgggcttcct
    35881 gcagcttgcg cagggcgtgc aggtggcagg gcagcccgac gaacacgtag cggccgggcc
    35941 cgggctcgat ctcccgcaag accccggcaa agtgagtggg gcagtaccgc gatccgctgg
    36001 cggctggaag gtccccgggg tcgcggatca cggccgcctc cggctcgagg ggccggccgg
    36061 ggacggatcg gatcgtgacc gcgccgtcta cccggcgtgt tttcagtagc caggccagca
    36121 agccggtcac gagcccgccg gacgagccga gacgccgcgt cgcgtcgtcg gcgcagtgcc
    36181 cgacgtaaca gcccgcgcag cggccgagcc ccgggacctc cgcgtcctcc ggaaacagag
    36241 cggcgccgag ccggctccgg tccacgccgg ggcctggaca tacttgccgg cacagcccgc
    36301 atcccgtgca gcggtccggc tcgacggcgg cttcgtaaag gccgccgcgc cgggtcatcg
    36361 aaatcgcgcc gccggggcag acgttttcac agatgccgca tcccgtgcag agacgcgccc
    36421 ccacgacgtc gcggatgctg gccggctttt cattcatgcg cacgaccggg cctgaagggc
    36481 ttccgcgaac acgtccccga gccggcaggc ctcctccgcg tcccggccgc tccgttccgc
    36541 caggcgcgcg cgcagggcgg cccgcccggc ccaggccttt tcgaccagcc cggcgaggtc
    36601 ggcttccggc gcgcggatgt cccacaccca ttcctgcagg cccagtccct ccatcacgcc
    36661 ggaggccttg tggctgtatt gcatgatgac cgagggcgtg ccggcggata cggcgctgat
    36721 gacggcgtgc atccggccgc cgacgaacag ctcgcatcgc ttcaggaggc ccttgtactc
    36781 cgccggcgag tagtcgcccg caagcgcgta cacgccggtc cggtcggaca tcgcgtccag
    36841 gaccagctcc gcggctgcgc ggtcgtcgcc gtcccacgag agccgggtct ccggggtcag
    36901 gccgggaaag cccgccgggt acgcctggtg ggg
//
LOCUS       DAOOTK010000023        34300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_924_length_34300_cov_6.031567, whole genome shotgun sequence.
ACCESSION   DAOOTK010000023 DAOOTK010000000
VERSION     DAOOTK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 34300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34300
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_924_length_34300_cov_6.031567"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1876)
                     /gene="murJ"
                     /locus_tag="PK634_04540"
     CDS             complement(239..1876)
                     /gene="murJ"
                     /locus_tag="PK634_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOW97037.1"
                     /translation="MTGTRKSAGIVGLAVVGSRVFGLVRELVFAAMFGAGKFLDAFLA
                     AFQIPNLMRDLFAEGALSVAFTTTFTKAHAKEGDESAWGLLSLLLSSVILILGGVCIL
                     GIAASPLLVHVTNFGFHQVPGKFELTVQLTRVLFPFILFVSIAAVIMGALNARHIFGL
                     PASASTVFNIVSIVLGVGLAYLFDPQPDWRHPQFGERALWGVSLGVLLGGVAQMAVQV
                     PALWRLGYRFRWRVNFRDDKLRIVWRLMWPSVIAGAAVQVNVLVNGMFASEIDGARSW
                     LNCAFRLMQFPIGVFGVAIATVTLPAVARHHARSDLAAFGSTVTSSLRLAVFLTLPAA
                     VGLFALAEPIIRLIYQHGRFSAADTANTALALRAYAFGLTGYAAIKVLAPCFYALDRP
                     RIPLRVSLLGIGVNLALNLLMVKVLGWGHVGLATTTAALAFINGTQLFIYLRRLVHPG
                     STGWFGFLAVVAAAAAACGFSAAAAHGRLLALLPAGLPGEILALCAAMAAAVAVYFVL
                     TLALRLPESAEVWDLAWRMLRGKTARPAVSEGPTKNP"
     gene            complement(1982..2458)
                     /locus_tag="PK634_04545"
     CDS             complement(1982..2458)
                     /locus_tag="PK634_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97038.1"
                     /translation="MKTPRFMLVLTTLCLAVTGYAGELSLIGHGSWVSASTPPILQWT
                     EDISVWMDVKVPNWSYHKTVGAVWTPDGWQTVRYAFARYEYTRGDGYEQWGIDIVPAG
                     VLGNSYIGPASWNGRSLLGGGKAEVEYALFYTLPSGYTLWDNNGGWNHHILIASRQ"
     gene            complement(2553..2909)
                     /locus_tag="PK634_04550"
     CDS             complement(2553..2909)
                     /locus_tag="PK634_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97039.1"
                     /translation="MKLEEKLAALEEIQTRMLQASTPEEKRKLTESLLPLMGEVIQLN
                     RADRQYRMTELARSLGLSDANAVRRFMGDVETIYRETQRDLAPFFSQGGPYHPSAPEP
                     YRTEPIPVVGPGQPPQ"
     gene            3408..5006
                     /locus_tag="PK634_04555"
     CDS             3408..5006
                     /locus_tag="PK634_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97040.1"
                     /translation="MKTFLMIALVSLGCLSVLAEDIPVANANALVAALARAESGDRVL
                     VSRGNYVGNFTVPPGVSLLGEPEFMPAGGLMPLFSPWGDTPTVVEQTRIGEKHPTQVR
                     GIFFTGAKMSALEVRGDGIGYVCHNMFEQNRGPRGGAIYSGAAGTIYIQTNYFSANTA
                     ETDGGAVFIGGTASVSLRMNEFLRNGAGTSGGGFAVRGTNAVASDRDFFMDNTAGVDG
                     GSVSVVLNPKPVILNRASLFGSEAGRDGGDVSVDRSLMMVLSGSSSGAMAGRHGGSLA
                     AIDGVIGVSGFNCNSARAAELGGGVYSEASLISCQGSEWTSCAARFGGGMAIRGMPAA
                     GGNPPSIQRNTFRQNRANKDGGALYLADLKEARIENNVLFMNTAGVGDGMAIDPDATA
                     LVDHNTVAHIEYRSGQPRPTAEAIRVRGAGNTTRVVNNIIAGHACGVIGDAGGRVQVL
                     GNDFWLTMLPWRDVPNPAGLLNRQVDPLFVRFDPRRLIGDLHLQVNSPCRNAGVVPIL
                     TTLDRDARRRDAQPDRGAYELPAP"
     gene            5746..8727
                     /locus_tag="PK634_04560"
     CDS             5746..8727
                     /locus_tag="PK634_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97041.1"
                     /translation="MKASVVACIVLAGMAVCAHAGRVFFQDFTNSTDWTSYRGANSNC
                     FNAFIASSGGSASNQFRVAEESLEAARTNYVSWALCRLTDLGVNSYAQYEIELNITTV
                     EQASAGAMNFYFGDGLHSNANAEVTTVLAMKFTVETLPGNRFRAYHANFSSPTNSGPV
                     KFTIIVNSGESPREYTDPLGRTQVVEGGNVDAWCGEQQWIDGLHKIKAGVIPREFKIH
                     SQSGFAVTRFDSFAVYNRPPGEESVYVWAASPQEGPGEEWSNAFHDIQSAIDAAPTGA
                     VIVVTNGVYAAGGCPAPGSGLTNRICITNAITVRSVNGPSSTFIVGSGPFGPAAARGA
                     YVGAGAALIGFTVQGGHTLTNGDELRDQSGGGVFCERGACVQECVLAENLAWRDAGGV
                     RGGALDGCELTGNTACRLGGGALGASLDNCLLQYNEAGDWSGGGGAAESVLSNCVVRH
                     NISAAGKAGGAWRCALAGCVVSGNTAAAYGGGASESTLRDCRVVQNQANEQGGGAAAS
                     ELLSCVVSGNWASVGGGAWECSLTNSTVSDNEAEFGGGAADCIGLDSCVLSGNRAAYG
                     GGAWGCVLTNCELAANTAMDGVGGGVATSLLFDCVLRGNFAAGNSVGGGAFESALHGC
                     RLDSNRSDGAGGGAYNSLLAACVLCSNMAVYGGGAATGALAGCTIVGNQATQGGGAWR
                     ADLAGCLVESNSAVDGGGAYEGRLVGCRMEHNEAANGGAVCGGVLFNCLLVNNRAAES
                     GGGGCHSELHNCTVVGNESAIGAGLCNGAAVNTILYANVCPEGQDHTNSALSHCRAPP
                     PADGPGNMTNDPCFLSAATGDFRLASSSCCIGTGVEEDWMTGPGCRDLAGLPRVVGDT
                     ADLGAYEYPYTSSGIYAPWLEQFGLPLDGSIDHQDPDGDGYSTWQEWRAGTIPTNARS
                     LFRLCWDDSGPPSGRVLRWPGAAGRRYAVDRTQDLLSPGLDRIASGLEGRDGFMTYTD
                     ATATADRPAFYRVLIEDEGGP"
     gene            8929..11943
                     /locus_tag="PK634_04565"
     CDS             8929..11943
                     /locus_tag="PK634_04565"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOW97042.1"
                     /translation="MYGGGRGIRVDDLPACPLRAKLESLVPAARARALRNLEAVSFHR
                     NDLASLRADREGGIFYACALESIREEPPGPTRPLQARAASLSVTNPPLFHSRPGSTNV
                     LFLDFNGHVITNTMWNSDEGIPRWDCPPFDEDSDETTFSDLEQRHIREIWERVAEDFA
                     PFDVDVTTAPPPAWHFRTGHALITSAYDAEGRETPLVYPGGTYGGLAYLNAFTNSAYS
                     YNADNCRSPAWIRQSLNLDLCVQIVAETISHELGHNLGLSHDGTASGVEYHAGFAATT
                     NAPSWGPIMGSVAGRDVTQWSRGEYSDANQKEDDLAIMALRLGYLADDHGASNGAATA
                     ISPAGYAFSVTGRVEQTLDRDVFALTTGVGIVTAAVYPFRATNLSWGANADLVLLLCA
                     SNGTVLASNNAPADVKAVLAPRPVAAGTYYLHVYPAGAGFPSNASSSGYTVYGCLGTY
                     RLAGAVAPPADTVVVTAPTHGSRLFLSNATEIAWLSGFTNPVRVELWRGGAMHTMIAT
                     NLANQGTHSWTVPASVTPGYGYRMRVARADSPAVCGESDGQAAVYGLPVSLFFENFDG
                     SGSLPAGWNEEFVTNTVSWRLQGGGGASGGNSPAAAYTGSNNAHFFSSPRAQTRLVLP
                     ALDIGDVLDPRLSYRLCMADIAMEQLRVLYRTNALAEWAPLTNYYGRYFTSWTQHSFP
                     LSNAAPDCALAFEGRAASSSYGICVDDVEVTGIVLAGELLTNRLEVASDLGLPDPPAG
                     TNWLSVNAPRTISAGTSPLTAGSTQFVCAGWTGTGDVPASGTETALAFTLYVHSSVTW
                     RWRTNYSFDCSAGDDGTVAGATSGWYAAGALITVTAAPNPYCDFTGWGGDAAGDTNDL
                     ALTVTLDRPRQLVANFTPRLAAHHGTPESWLAEHYPEAGDLDAVEISDTDGDGLEAWQ
                     EYIAGTLPGDALSVLELESGGAVSEGQWLLRWPAISGRSYSLWFFTNPISPEDVPVVT
                     SRLAGADGVMACTNAIPPEILRGFYRLTVRRE"
     gene            12230..12604
                     /locus_tag="PK634_04570"
     CDS             12230..12604
                     /locus_tag="PK634_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOW97043.1"
                     /translation="MNCPACKASMAILEYSGVELDFCFSCKGCWLDAGELGLILTGAP
                     DRPDDLKLEKASKGKRRCPRCGSRMLVGPAPATGVEVDACPAGHGFWLDAGELEQIVQ
                     ARGASGRAAAVAKFCADVFGKK"
     gene            12623..13174
                     /locus_tag="PK634_04575"
     CDS             12623..13174
                     /locus_tag="PK634_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOW97044.1"
                     /translation="MGLWIVIGVIVLVVLAYIGMYNSLVSLRNQVKNAWSQIDVQLKR
                     RHDLIPNLIETVKGYVKHERDTLENITKARTNAMQASGAAAAGKAEGELSGAISKLML
                     VVENYPELKANQNFLALQEELSSTENKVGFSRQFYNDAVLRFNTKVETFPSNIVANMF
                     GFKQAEFFEIQVATEREAPKVQF"
     gene            13255..14499
                     /locus_tag="PK634_04580"
     CDS             13255..14499
                     /locus_tag="PK634_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOW97045.1"
                     /translation="MWETIQSNKRKSAVMIVLLATVLMFVGYALGAALDPAAGVLGVL
                     AAMVIWVVLTLTAFAGGEKMLLASAGAREVSREQAPQLYNLVEEMKIASGLPAMPRIF
                     IMDSDVPNAFAVGLKPERAAVAVTTGLMARLNRDELQGVIAHEIGHISNRDTLFMTLA
                     GVMVGAIVILADVFRRSLWYGGGRRRSSSSSKGGQAQAVIAIVAIVVAILAPILARLL
                     YFACSRKREYLADASSAQFTRYPEGLASALDKISQFKPGALKVNQAVSPMFIINPLAA
                     TGGGLSLFSTHPPTADRIRVLRGMGKSASLAAYEEAFRAAHKGSGVIASRDLGASVEA
                     AAREGAAGAADPAAQWRSARDLLHQVNRFLVLPCACGLKIKLPPDFKDSSVTCPRCGT
                     KHEVPVAELAAASAALEAAGRK"
     gene            14496..15779
                     /locus_tag="PK634_04585"
     CDS             14496..15779
                     /locus_tag="PK634_04585"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOW97046.1"
                     /translation="MTGPRAILIRGARLLDPVRNLDAPGDLFLAGGLIAAPPLRIPPD
                     AEIIEARGLAVAPGFIDLHVHLREPGGEEAETIETGCRAAARGGFTTVVAMPNTRPPL
                     DTPELVSAQIRRAEEAGLCRVRPAACITSGRTGGALAPLAELAAAGACAFTDDGTTVA
                     DEALMRRALEQAAALGRPILDHAQDPAREKQGVMHEGAWSARWGLPGIPSSAEIEVVE
                     RDIRLARETGGRVHIQHVSAAGSAALIRRARAAGLPVSGEATPHHLALTDADVRPEDA
                     RWKMNPPLRSEADRAAVLGAVEEGTITVLATDHAPHTAAAKARGFRDAPFGVIGLEAA
                     VGVTYTRLVAGGRMSALDWVRRWTSGPARVLGLPEPSLAPGLPADVALLDLDTEWEIR
                     PESFASKSRNTPFEGWRVRGRAVRTFLGGRTTARA"
     gene            15841..16767
                     /locus_tag="PK634_04590"
     CDS             15841..16767
                     /locus_tag="PK634_04590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97047.1"
                     /translation="MKRPSVWTLVLGWNHAEDTIECLRSLQASEGVDLKLFFTDNGSA
                     PDQVERVCREVPAATVIRHPQNVGVPRGFNAGIAYALQHGADYLFMANNDTTVAPDTI
                     RILVEAAERDPRAGIIMPRIFYYDHRDLVWSAGSRFRRFPPAIVMRKGRGGFEREEDI
                     EFSSLCTVLLRGRMFKDAGLMDPNFVFYYEDYDLCLRIRDAGYAIKLVPAARSWHKVA
                     RITRAGSTSPTFWRNYGRSVAIFQRLQRRRRWLAGPAFLAFQYFRALVEGKRQAWAPF
                     REGLREGRALELKPVPRWDQPPMDPVEVAQAP"
     gene            16820..17815
                     /locus_tag="PK634_04595"
     CDS             16820..17815
                     /locus_tag="PK634_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97048.1"
                     /translation="MIILPAMKLVVQIPALNEEKTIQDVIRSIPRNIPGIRSTSVLVV
                     DDGSTDRTSALAAEAGAIVVRHDRRRGVGAAFRTGLQKSMELDADVIVTIDADGQFNP
                     EDIQKLTEPILRGEADYVTASRFLDPSLTPEMPRMKRWGNDFMARWISSLVGQDFKDV
                     SCGFRAYARDAYLRLVLLGDFTYTHETFLTLAFARLRIREVPILVRGVREHGQSRVAS
                     NLWRYGWRTAGIILRTYRDYRPLRFFNYVAGVLLVAALGFLGFLISVKVRTGMFTPHK
                     WSGFLAAALAGAALVLFLIGLVAEMLDRLRVAQDELVFRVRRMEMDLKRAKQQPS"
     gene            17832..18968
                     /locus_tag="PK634_04600"
     CDS             17832..18968
                     /locus_tag="PK634_04600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW97049.1"
                     /translation="MAKPLVLSYWNVNRPVTGGLRRVTALLEALGPDLVLCQPEPAHP
                     VYETAAYRTNWTRAGGGGINRGLFHFFLPEPARAAREAWRRVRPGLIVHTSIWTFWPL
                     RRAGTPMVLDAHDALGAAMTGRYGRRHPFTLLVRAWERRVARRVDRLFVCSDVDRAYF
                     AREYGVSEDRLSVVPNGVHVRPPAEGPLPAGWEGRLAGKTVLFFMGKPAYQPNAQGLQ
                     FLRDTVLPELERRQPGRFCVVACGGEARPGMAHPALHFAGVVSDEELQAFIRRADLCL
                     SPTFSGSGTRLKILEWLAAGKAVISTPKGAEGLGAVPSRDLVEAEGRDFAAAILSLAE
                     APDRVRALGRAGADFVRQRYDWSSSSQPLWREGMRRWLDPRQTG"
     gene            18965..21166
                     /locus_tag="PK634_04605"
     CDS             18965..21166
                     /locus_tag="PK634_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97050.1"
                     /translation="MNGVPASRPRRAAAFLLRQAPLLLVGAVILWGACRIQTYICAQD
                     PSTYIDQALRLLRAPWGSPEMWQALTRYSPLHTLVLAGSIRCFGTMAPYWVNVFFAWA
                     FLACLWWFLARLWPSAGDRALALAVAVTCLLAGHDANIQFLVYPFREASAFFFIFLSY
                     GLLLKGDAEDRRSFSALAGLAMLLGVAAREPFVMAVPGPLLLAVTGRGRPWARRLAHA
                     GLFLLPFLLAGGAWFTVTAMTGQGTTAQFKDWAELMQSHGAGTFAARTLGNVRYLAGV
                     ILGEIGWSGVALLAAGLYPGRRARIVYLLLVVPALTVGLFYSTYNIYMRYVMSVLLFL
                     GPWAGTGLLNLLALARFLARGTRLRLAWAPLAGSLLLLGSLAVQLASLPASPFTRREV
                     GAFLGRLSGLGTGTVYYVERMNRDIWAALRNHTHLETSRALDVPRNLEAGRPMAFLKP
                     LNAASRARAKGREDGVRIEELLLQSGDLVPWPGVVEPWRLGRGAYAVLQVVPRSRTSV
                     SEPVAIPAGTAGVLWLNFRDVRPEAVKKIRLLGPDGRERLATEMMGQGLQPVWWPGDP
                     AEETNLVLETASSAPLPARVYTGFQPAAEAREFPLDARRLLSATRWFEPPFGQAPARF
                     SPAVFFSEGGRLRLPWPNDPRADRLQLSLEGRVPPDREKTGQLTVRSGPREVPCEANR
                     VGYRSWNIDLTIERESGAREAVLELSYEPVNTNRPLHLTVSQAALRYFGSE"
     gene            complement(21148..23817)
                     /locus_tag="PK634_04610"
     CDS             complement(21148..23817)
                     /locus_tag="PK634_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97051.1"
                     /translation="MLHPWRHKILSGPGARRLAAVLSALTRPGALIAFVVAGLAWFVW
                     NYYYLKPYSDPLNWLTLAHNLHPTDHLQRFPLAYPVFLRFVIRLVGPFYVFVSNLPLL
                     VAIVLLVARLTVLAARECMDARAAAYAGVAAAAGLLYFDSEILVYLTNPYRDPLSHVL
                     LLTSVLLLIAYLGRGGRPWIALVLSALALGLATDVRETAVLMAPVMGWYGLSAWRKNR
                     AIPLGRSVILFGAFLALGVLPLVLQSYFRTGHAVVPPQAVVEGRLLPGLHLRAFVPTL
                     IHALRYFWWRGGWLYAAGLVYGIGLAVVRRRSLLRDLLLPAALVHFIFYCFYWVFVKR
                     YFFIVVLWSVPLAACGALALAERLLCAAGKQRWILPALRSLAALFVLVAVIPLLRPPE
                     MPPFQIPQARRLKADIEKAVPRDAVILTPPGLGGLVSYFTRLEAYAIPILNPTNLEVE
                     IHQHVRERLAAGHPLYLMDSTDDPFGRIVRQLFDTETMARAPADRYHFQPAVGLGDLA
                     LSRLSLWTNTATSLTLEAAPDADDILLFNGATLWLEGVPRRWARLSLNGRVIAPRVGN
                     GTQYYRVDWPSFTPPYRLTLESDQPVPNTFGAGLVRRDEPLVLRFASSGDTVHDAVLE
                     EGFTILKGQTRRKPHWPARATVAVPMPYPPNDLIQVSFYLRVRPTTKESLEVPVDVRF
                     TPDGQPPETRRILRSYIFHRRVLRIPAGAREGQVCRIGLETLDVMSGAPEPPFPPELD
                     RITVHDLSAIRSVDIGSGVDFPHIVSGFYAREGRGEDTMRWTDGKGVLAFWLAADGTD
                     RVVRVDFKPQIRATNAPPAEIALSFNGTDLAAHLVDSNRVEAAIPATLVQPGTNTLTV
                     SSTTWSPHELLGVGDSRQLGIPVDRVGFYSEPK"
     gene            complement(23832..24911)
                     /locus_tag="PK634_04615"
     CDS             complement(23832..24911)
                     /locus_tag="PK634_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOW97052.1"
                     /translation="MKPIVKSIILRVVLMLFVLFCALAALELALRLRGPMPESIKQTP
                     LFDRSRFYFASDAERIHPWVRDTNHVLRVAIVGDSFTTGQGVQPDDTFGMRLERLLNL
                     KKDATPAAVDVFAKGGTSTYMQRGFLDEALATRPRLVILAMYLNDTENFNNYKELRAW
                     RTEMMPPVPTGWLAPLIRRSRALAWFNKKIGDVKSGRGFHRYYERLYDDDYSGWKLFQ
                     DAARDFHKACRDQQVEFLVVIWPCLSFVDRTPYPFQFAHDKIIAHLQSENIRYLDLLD
                     SLRGRSPTRMETLPGADGHPSEIAHRIAAEAIFHYLLNEGLVDAAYIPRNEALQLNKY
                     WEAMYERMAKPESARSEVEPQAPVE"
     gene            complement(24962..26008)
                     /locus_tag="PK634_04620"
     CDS             complement(24962..26008)
                     /locus_tag="PK634_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW97053.1"
                     /translation="MILAFCELGLRAYYRVAPDQQLRHDRPPVSFYDGETRMHPWSRG
                     ATNALRIAVLGDSITVGAGSQPQDAYGARLERLLNLNDGQRPAEVRVWARGGMCTFGE
                     LKFLEEAVAWKPELLILGICLNDTEDPRRKEELTRWRMDALPHQPSRWFARSLGWSRL
                     ATLVYQKLEDARARRGYLEMNRKLFDKEYGGWKLFTKALKDFSETTRRNNIKMVAVVF
                     PLLSHIDDYPFDWIHEQVHEELDAAGIPYLDLLPVYKGLPSERLQAIPRVDAHPNEIG
                     HRIAAEAILKYLLAEKLIYPEYLPAIASRSPEEQWRILSAFMNDVTAVKQDEADRLAG
                     HGEPVAEPAPLTPE"
     gene            complement(26601..27758)
                     /locus_tag="PK634_04625"
     CDS             complement(26601..27758)
                     /locus_tag="PK634_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW97054.1"
                     /translation="MGLRILMLTWNYPPVTGGIEYVAEHLVRGLRARGHAVRVVTAHA
                     KDAPPEPDTWRAARPGLKAYVLFSLARGFSLCRGERPDVIACSSVVSAPAGWMLSRLF
                     RRPLVVLAHGSDILYPGWLYTRAVRWLFGRADRLCANSGRTRDLLIAAGQRPERIVVV
                     PPGVLVPPADVSPPPLPKELSDLVAGRRVLMTVGRLIRRKGVLEFVEQVMPPLVREFS
                     DVLYVVVGDDAHHSLVHHERRRDLIARRVQELGLARHVHLAGRLRDEEVAALYREARV
                     FALPARDIPGDVEGFGIVFLEAALAGLPSVATRVGGILEAVEDGVTGRLVEPDDAEAM
                     RAAVAALLRNDTERRRLGEAGRQRARDQFSWEVISARYETVFQDLAGGKAT"
     gene            complement(27760..29967)
                     /locus_tag="PK634_04630"
     CDS             complement(27760..29967)
                     /locus_tag="PK634_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97055.1"
                     /translation="MDPKPHVPAPRLAPLAAWLFALGILALAAWQIRTAVSGQDDATH
                     RIFARTILSADRSSPEFRQALTHVAPGWPLFLALAIRVAGPWVTAWINVALAGLLLGL
                     YLLVLRRVFGDDRAAGLGLLATLLILLRGYVLNAHFLLYAFRETPSFLFVAAGWLLFL
                     LADDRRKPGLFFAGGCAFVIAAAMREPAVCGAIGPYLWLLFRRKESGRARRACFFGAI
                     LPWLLAGGTWLLARQAVGSPSSGQWQWWSARLLHTPPPAFRDAYVHSLRALLVFLRDA
                     LGYPWLLLGLLGLAGLRRNRAALWFFLFSALAYLFLYATYTAHRRYALAVLLPLCPFA
                     AYGLHLAIDKLSVLAVRLVRRPIAGPARVLAAALLAVGLIRTAWTLQPWGPRITRQDV
                     EQFRRDFDAVVRPEDLVVIEPICRHLGETMIYHLAHRPADPARAGMDGGGARCFYLEP
                     LTRDCFPPGSFRRDLANSASRHLRYRHDVQARVGPDGQPLQLALGGGRYGVYDVRPWS
                     ATSVSARVTLPAGDTAVLWLDFKDGPSNAARRVEVRTDGGQTLASWLVEDARFLQAFI
                     FEKPAEPGAALVDVSGDRPGPSGFVYTVVPAGKPARFEAGPQRGPSLERWFEPAFVGA
                     ALAAPASSGGRLRLPAVHGSYGAFNLALGMKAAPFQGTVSFRYEQDGRAVTNWAVALP
                     QSRIWTGAILDPQQAARPLDLFIDAPGGTTLWFATETVQAAIEPAAAGRNETE"
     gene            30094..30615
                     /locus_tag="PK634_04635"
     CDS             30094..30615
                     /locus_tag="PK634_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOW97056.1"
                     /translation="MRRSCQAGGITCALLAAGWLATAWSGEVPRVEVGGATVSEETPA
                     VRIVRKIARPAAVGRPVDVVVSSGESPQTNGWDVADGDLETAWAGRAGENGWWLALVY
                     EPPLKMTDIELLLAPSSTTNVMILGSADALDWIELPAAPESGLDRIRYLWIVFEPDAS
                     GRRPVVLEVKPIP"
     gene            30666..31925
                     /locus_tag="PK634_04640"
     CDS             30666..31925
                     /locus_tag="PK634_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97057.1"
                     /translation="MRVTAVLKECVRQEVEARASPSRQRRLQSARLARLVARAAVRVP
                     FYREAFAAASLDPAIVRGLDDLARIPVTRKLEYRRCPPGERLAAGVRPEDCRVEHTSG
                     STGIPLNVYLSEGEQAQRSALWLRARLRCGLWPWRRHLLVGYYQPPPVPWFLRRRRIH
                     ATAAEACAILGHFRPQVIGAYTSQLLLLAVELQRRGIPPPRPRVLRTGGETLSPAARQ
                     RLEQFFGAPARDFYAAVEFGLIGSSCPARGGYHLASGDLLVEIVRDGRPAAPGEEGEV
                     LVTSLVAHAMPLIRYEIGDVGALAPLESCPCGNPFPRLAGLSGRSEDMIVLPGGRRAS
                     PRLAAIPFWENTSILQYRVTQRAVGEFEVEVVPDGPLDPGLADRVRSYFREHFEAART
                     DIRVRDRIDPDPQGKLRKVVVLASDGR"
     gene            complement(31911..33527)
                     /locus_tag="PK634_04645"
     CDS             complement(31911..33527)
                     /locus_tag="PK634_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOW97058.1"
                     /translation="MNGAGTGWRAGARKAAGLVSGWIPGGGPLLRAATRAWLAYRSDV
                     PVILISGSSGKTTTCLLLQSLLAERCETVGTRDNDNIDCHQHRFVFRLGARRRRQILV
                     LEAGIQSASNAPRWNAAVASDVLILTMIGHTHLQWLHSREGVFEQKKALLGALKPGGT
                     LLVNADDDLLGTLRSDPRAKTFGVEQEADFRARNVEADARGVRFDLVREGRVLARIES
                     ELLGRHAVYPLLAAASCASLFGLDAEAMRRGLARFRPAPSRVSVGTNREWTVLDDTYS
                     SNPEAAWAALEAMHLLDGRKVVVLGSMLELGERGPDLHRELGRRLARAFPGIQRLFGY
                     APLSEFILQGAVEAGFPAERTAQTDSKFELLARLEDELRPGDVALFKGSNQLALGDVI
                     RRLGRPWFAPPIEAMPAPRAVGAFGAYRIADRRHEGIDLPAEPGTPVRAMADGEIRRA
                     GWNGAYGRAVRIRHFTNTVSLYAHLDRIVRRRGPVAMGEIIGYSGRTGIPRGLGDYDY
                     PHLHVELRVNGVAQDPSVYWDRFRAGSPAV"
     gene            complement(33524..>34300)
                     /locus_tag="PK634_04650"
     CDS             complement(33524..>34300)
                     /locus_tag="PK634_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOW97059.1"
                     /translation="PPPPIRVLPDPAVGLQPAAESEARAELEHQGVPIRPDEVRIGIG
                     LRRWFHIGRRFFPLQWKWRLTGRRPEAPPTHEQLLHNLAGALGRFSEGRRLRLLFFPM
                     YAVPWEGDDLESLDLGRRLRLPSHILNLDIPARRIKALTGLCDLFVGVRLHSAILALG
                     MNVPTLGIGYVRKSSNLFERLGLEEQLVDIAAAAGPGGGDLLLGKLARLHGHGQEIRS
                     EQAARWAPLAAECQSGYADLVRDARSGSLSRRAAGAGSGP"
BASE COUNT         5381 a        11530 c        12104 g         5285 t
ORIGIN      
        1 accaatatga aaaacagacg cttgaagatg taagcaaatt aattcatatc agaaaagatc
       61 acccggcttt acgaggacga aatccgccgc cgccccgagt gctggctgtg gacctacaag
      121 cggtggagga tgatccggcc gggagacgat gtccgagcct atccgttcta cgccgtgcag
      181 cgaaggaatg gagtgcgacg gacctccgct gccgagcggt cgccggcggg cggcgtccct
      241 acgggttctt cgtagggcct tcgcttaccg cgggccgggc tgttttccct cgcagcatgc
      301 gccaggccag atcccacacc tccgccgact ccggcagccg cagggccagc gtcaggacga
      361 agtagacggc gacggccgcg gccatcgccg cgcagagcgc caggatttcc cccggcagcc
      421 cggcgggcag gagcgcgagc aggcggccgt gcgccgcggc ggcgctgaac ccgcaggccg
      481 cggccgcggc cgcgacgacg gccaggaacc cgaaccagcc cgtgcttccc ggatggacca
      541 gacggcggag atagatgaac agctgtgtcc cgttgatgaa agcgagcgcc gccgtggtgg
      601 tcgccaatcc cacatggccc cagcccagga ccttgaccat caggaggttc agcgccaggt
      661 tcacgccgat gcccagcagg ctcacgcgca gggggatgcg cgggcggtcc agcgcgtaga
      721 agcacggggc cagcaccttg atcgccgcgt atcccgtcaa cccgaacgcg tacgcccgca
      781 gcgccagcgc ggtgttcgcg gtgtccgccg cggagaaccg gccgtgctgg tagatgaggc
      841 ggatgatcgg ctcggccagc gcgaacagcc ccaccgccgc cggcagcgtc aggaacaccg
      901 ccaggcgcag cgaggaggtc acggtcgacc cgaacgccgc gaggtcggac cgcgcgtggt
      961 ggcgcgccac ggcgggcagc gtgaccgtgg cgatggccac gccgaagacg ccgatcggga
     1021 actgcatcag ccggaatgcg cagttcagcc acgaccgcgc cccgtcgatc tccgaggcga
     1081 acatgccgtt gaccagcacg ttcacctgca ccgccgcgcc ggcgatcacg ctcggccaca
     1141 tcagccgcca gacgatccgc agcttgtcgt cgcggaaatt cacgcgccag cggaaccggt
     1201 agcccagccg ccacagggcg ggcacctgga ccgccatctg cgccacgccg cccagcagca
     1261 cgcccaggct gacgccccac aaggcccgct cgccgaactg cggatgccgc cagtccggct
     1321 ggggatcgaa gagataggcg aggccgacgc cgaggacgat ggagacgatg ttgaaaaccg
     1381 tggaggccga cgcgggcagg ccgaagatgt gccgcgcgtt cagcgcgccc atgatcaccg
     1441 cggcgatcga cacgaagagg atgaacggaa acagcacgcg cgtgagctgc accgtcagct
     1501 cgaacttgcc cgggacctgg tggaacccga agttggtcac gtgcaccagc agggggctgg
     1561 ccgcgatgcc gaggatgcac acgccgccga ggatcaggat caccgacgac agcagcaggc
     1621 tcaacaggcc ccaggcggac tcgtcgccct cctttgcgtg ggccttggtg aacgtcgtcg
     1681 tgaaggcgac cgacagcgcc ccctcggcga acaggtcgcg catcaggttg gggatctgga
     1741 aggccgcgag gaaggcgtcg aggaacttcc ccgcgccgaa catcgcggcg aagaccagtt
     1801 cgcggaccag gccgaagacc cgcgagccga ccacggccag gcccacgatg cccgccgatt
     1861 tccgggtgcc ggtcatgccg gaccatagca cggcggcaaa accgctttca atgccggcct
     1921 ggtttgaaaa aagaaacgcc cgccgggtgg agggccggcg ggcgtggagg aagatgagaa
     1981 gttattggcg gctggcgatc aggatgtgat ggttccaccc gccgttgttg tcccagagcg
     2041 tgtagccgct gggcagggta tagaagagcg cgtattccac ctcggccttg ccgccgccga
     2101 gcaggctgcg gccgttccat gaggccggtc cgatgtagct gttgcccagg acgccggccg
     2161 gcacgatatc gatgccccat tgctcgtagc cgtccccgcg ggtgtattcg tagcgcgcga
     2221 aggcgtagcg aaccgtctgc cagccgtcag gcgtccagac cgcgccgacg gtcttgtggt
     2281 aggaccagtt cggaaccttg acatccatcc agacgctgat gtcctcggtc cattgcagga
     2341 tgggcggggt gctcgcggaa acccaggaac catgcccaat caagctcaac tcaccggcgt
     2401 atccggtaac ggccaggcac agcgttgtca gcacaagcat gaaacgcggc gtcttcattt
     2461 ccatatcctt tcggcaacag agtgtagcgt tttactaaat cgctctacta gtacgcaggt
     2521 cagctgctgc tgttaagtct tttttaaact ttttattggg gaggctgacc ggggcccacc
     2581 accggtatcg gttcggtcct gtagggttcg ggagcagaag gatggtaggg gccgccttgc
     2641 gagaaaaagg gcgcaaggtc ccgctgggtc tcgcggtaga tggtttccac gtcgcccatg
     2701 aaccggcgga ccgcgttggc atcgctcaag cccagcgagc gcgccagttc ggtcatccgg
     2761 tactgccggt cggccctgtt gagttgaatg acttcgccca tgagcggcag caggctctcc
     2821 gtcagcttgc gtttttcctc cggggtggaa gcctggagca tccgtgtctg gatttcctcc
     2881 agcgcggcca gcttttcctc gagtttcacg aggatagccc gctcgttcgg atcgcttgca
     2941 gacgccagcc ttcttttcaa gcggtccgct tcaagagccc gcgcatcctt ctgcgcggcc
     3001 ggaagcggtc cgctttccgt cgcggctggg gaggggatgg gcgcggcggg cgccggagcg
     3061 gcttcgatcg gtagcgggcg ctgcgtggac gccgccgtgg ccctgacaag ttcctgccgg
     3121 agttcctcca gttggcctcg gagccggagg acctctgcgg ggggagacaa gatctcgcct
     3181 gtggccgggt cttggacgga ttgcgcaacg cgacagggcg accgggtcca cagtgcccat
     3241 gtcaggccgc ccagcgccgc cgcggcgaca agccagggga tgatgattcc agcctttcgc
     3301 atgtcccgca tcataaagag cccgcgccgg gcgcacaagc gcgggccggc gggaagatcc
     3361 gccgagcccg gacgacatga gcagataggc aagaggggag aacattgatg aaaacgtttt
     3421 tgatgatcgc cttggtgagc ttggggtgcc tgtccgttct ggccgaggat attcccgtgg
     3481 ccaacgccaa cgccctcgtg gccgccctgg cccgcgccga atcgggcgac cgggtcctgg
     3541 tgagccgcgg taattacgtc ggcaacttca cggtgccgcc gggcgtttca ctgcttggcg
     3601 agccggagtt catgccggcg ggcgggctca tgcccctgtt ttcgccctgg ggcgatacgc
     3661 ccaccgtcgt ggagcaaacc cgaatcgggg agaagcatcc gacgcaggtg cgcggaatct
     3721 tcttcactgg cgccaagatg tcggcgctcg aggtgcgggg cgacgggatc ggctatgtct
     3781 gccacaacat gttcgagcag aaccgcggtc cgcgcggggg cgcgatttat tccggggcgg
     3841 ccggtacgat ctacatccag actaactact tcagcgccaa cacggcggag acagacggcg
     3901 gggcggtctt catcgggggc acggcgagcg tctcgctgcg catgaacgag ttcctgcgca
     3961 acggggccgg cacgagcggc ggcggcttcg ccgtgcgcgg caccaacgcg gtggcctcgg
     4021 accgggactt tttcatggat aacacggccg gggtggatgg cggttccgtg tccgtggtgt
     4081 tgaatccgaa gccggtcatt ttgaaccggg ccagcctgtt tggcagcgag gcgggccgcg
     4141 acggcgggga tgtgtcggtg gatcggtcct tgatgatggt cctgtcgggc tcctcgagcg
     4201 gcgccatggc gggccggcac ggcggctcac tcgcggccat cgacggggtt atcggggtga
     4261 gcgggttcaa ctgtaattcg gctcgcgccg cggagttggg cggcggcgtc tattcggagg
     4321 cgtccctgat ctcgtgccag ggcagcgagt ggacttcctg cgcggcgcgg tttggcgggg
     4381 ggatggcgat acgcgggatg ccggcggcgg gcggcaatcc gccgtccatc cagcggaaca
     4441 cctttcgaca gaaccgggcg aacaaggacg gcggcgctct ttacctggcc gacctcaagg
     4501 aagcccggat cgaaaacaac gtgcttttca tgaacacggc cggcgtaggg gatggaatgg
     4561 ccatcgaccc cgacgcgaca gccctcgtgg atcacaacac cgtcgcgcat atcgagtacc
     4621 gctcgggcca gccccggccg acggccgagg ccatccgggt tcgcggggcg ggtaatacca
     4681 cgcgcgtggt gaacaatatc atcgccgggc acgcctgcgg cgtcatcggg gacgctggcg
     4741 gccgggtcca ggtcctgggc aatgatttct ggctcacgat gcttccgtgg cgcgacgtgc
     4801 ccaacccggc cgggttgctg aaccggcaag tggatcccct gtttgtccgg ttcgatccgc
     4861 ggcggctgat cggcgacctg cacttgcaag tgaactcgcc gtgccgcaac gcgggcgtcg
     4921 ttccgatcct cacgacgctg gaccgggacg cgcgccgtcg cgatgcccag cccgaccgcg
     4981 gcgcgtacga attgcccgcg ccctgaacac gacgcggagc tattcctcgc acgcgcccac
     5041 ctctacgggc cggcagcgcg cgccgtccgc gtaccggccg ccgctgaagc aaacggcgga
     5101 atgtcacggc cgtttcctgg aaggcgaccc gtcgtccccg ccgcgtttcg cctccgcccg
     5161 ccttcgcgga tgcaactgcg gcgggcaagc aaggccgcgc cccccgctgg aaatggccaa
     5221 agcacttctg aatccgcttc agcgtccttc ctctgcggct ggccggccgg ctgctcgtcg
     5281 atggcagccg tccatattgg ctctgaacga accgacttca ggaccgggct tgatgcgcgg
     5341 ccatttctct ggcctcgcta ttgtcccttg tacgtgactc ggctatgtca tgcgcgacct
     5401 cctttccacc tgcggtccgt tcaccccggt tgtttcatct cggaactcgc cgcgcccctg
     5461 gaatgacgag ttccggctcc atctaataga aagtaagcag gggggcggaa ttccatgaaa
     5521 aacgaggcgt gcgcaactcg tgtcatgata atcggttacc ttggggcccg aaatgagcct
     5581 gaaaaaatgg cgcgggaatg cttgaccgat gttctgccgt gcctaaactg caaaatcacc
     5641 tcgtggtaaa aggagcatca ggaggtgttc cgggggcggc ggcgcggagg tcgaattccg
     5701 acggcaggac aaggcttccg cgcgcctagg gatcaaggag tcggggtgaa ggcatcggtt
     5761 gtcgcatgca tcgtgctggc cggaatggcc gtgtgcgcgc acgcggggcg cgtgttcttc
     5821 caggacttta ccaactccac ggactggacc tcctatcgcg gcgcgaattc caattgcttc
     5881 aacgccttca ttgccagcag cggcggcagc gccagcaacc agttccgggt cgcggaggaa
     5941 agcctggaag ccgcgcgcac caactatgtt tcctgggccc tctgccggtt gacggacctg
     6001 ggcgtcaaca gctacgcgca atacgagatc gagttgaaca tcacgacggt ggaacaggcc
     6061 agcgcggggg cgatgaattt ctacttcggc gacggcctgc acagcaacgc gaatgccgag
     6121 gtcaccaccg tcctcgcgat gaaattcacg gtcgaaacgc tgcccggcaa ccgcttccgc
     6181 gcctaccatg cgaacttcag ttcgccgacc aacagcggcc ctgtgaagtt cacgatcatc
     6241 gtcaactccg gcgaatcgcc gcgggaatac accgatcccc ttggccggac ccaggtcgta
     6301 gagggcggga acgtggatgc ctggtgcggg gagcagcagt ggatcgacgg attgcacaag
     6361 atcaaggccg gcgtcatacc ccgggaattc aagatccact cgcagagcgg tttcgcggtc
     6421 acgcggttcg attccttcgc ggtgtacaac aggccgcccg gggaggagag cgtgtacgtg
     6481 tgggcggcca gtccgcagga gggcccgggc gaggaatggt ccaacgcctt ccacgatatc
     6541 cagtcggcga tcgatgccgc gccgacgggc gcggtcatcg tcgtcacgaa cggcgtgtac
     6601 gcggcgggcg ggtgtcccgc tcccgggtcc ggcctcacca accggatctg catcaccaac
     6661 gcgatcaccg tgcgcagcgt caacgggccc tcgagcacgt tcatcgtcgg cagcggtccc
     6721 ttcgggccgg cggccgcgcg gggcgcctac gtgggcgccg gcgccgcctt gataggattc
     6781 acggttcaag gcgggcatac cctgacgaac ggcgatgaac tccgggacca gtcgggtggc
     6841 ggagtgttct gcgagcgcgg cgcctgcgtg caggaatgcg tcctggcgga gaatcttgcc
     6901 tggcgggacg cgggcggagt ccggggcggc gcgctggatg ggtgcgaatt gaccgggaac
     6961 acggcgtgcc ggctgggcgg gggagccctc ggggcgtcgc tggacaactg cctgctgcag
     7021 tacaacgagg cgggcgactg gtccgggggc ggcggcgccg cggaaagcgt gttgagcaac
     7081 tgcgtggtgc ggcacaacat ctccgccgcg gggaaggcgg gcggggcctg gcgttgcgcg
     7141 ctggccggct gcgtggtcag cggcaatacc gccgcggcgt acggcggggg agcgagcgag
     7201 tccacgctgc gggattgccg ggtggttcag aaccaggcga atgaacaggg cggcggcgcg
     7261 gcggccagcg aactgctctc ctgcgtggtt tccgggaatt gggcgtcggt gggcggcggg
     7321 gcctgggagt gcagcctgac caacagcacg gtttcggaca acgaggcgga attcggcggc
     7381 ggggccgccg actgcatcgg cctggactcc tgcgtgctgt cgggcaaccg tgccgcgtac
     7441 ggcggcgggg cctggggatg tgtcctgacc aactgcgaac tggcggcgaa cacggccatg
     7501 gacggcgtcg gcggcggggt cgccacgagc ctgctgttcg attgcgtcct gcgcggcaat
     7561 ttcgcggccg ggaattccgt gggcggcggg gctttcgaga gcgcgctgca cggctgccgg
     7621 ctggacagca atcgctcgga tggggccggc gggggcgcct ataattccct gctggcggcc
     7681 tgcgtgttgt gcagcaacat ggccgtgtac ggcgggggag ccgcgaccgg cgcgctcgcc
     7741 ggctgcacga tcgttggcaa tcaggcgacg cagggcggcg gggcctggcg ggcggacctc
     7801 gccggctgcc tcgtggagag caactccgcc gtagatggcg gcggcgcgta cgagggccgg
     7861 ctcgtcggct gccggatgga gcacaacgag gcggccaacg ggggcgcggt gtgcggcggc
     7921 gtcctgttca actgcctgct ggtgaacaat cgcgccgcgg agagcggcgg gggcggatgc
     7981 catagcgaac tgcacaattg cacggtggtg ggcaacgagt cggccatcgg ggcggggtta
     8041 tgcaacggcg ccgccgtcaa caccatcctg tatgccaacg tttgccccga agggcaggat
     8101 cataccaaca gcgcgttgag ccattgccgc gccccgccgc cggcggacgg acccggcaac
     8161 atgacgaacg atccctgttt cctgtcggct gcgacggggg acttccggct ggcgtcctcg
     8221 tcctgctgta tcgggacggg tgtcgaggag gactggatga ccggcccggg gtgtcgcgac
     8281 ctggccggcc tcccccgcgt cgtgggcgat acggcggacc tgggcgccta cgagtatccc
     8341 tatacttctt ccggcattta tgcgccgtgg ctggagcaat tcgggttgcc gctggacggt
     8401 tccatcgacc accaggatcc ggacggcgac ggctattcca cgtggcagga atggcgggcc
     8461 ggcaccattc ccacgaacgc gcgctcgctc ttccggctct gctgggacga cagcggcccg
     8521 ccgtcgggcc gcgtgcttcg ctggcccggc gccgccggcc ggcgctacgc ggtggatcgc
     8581 acccaggatc tgctgtcgcc cggcctggac cgcatcgcgt cgggcctgga aggccgcgac
     8641 ggcttcatga cgtacacgga cgcgaccgcg acggccgacc ggccggcctt ctatcgggtc
     8701 cttattgaag acgagggcgg cccctgaccg gccggcctat cgctccgcgc ttctcccggg
     8761 taacctgatt tccagaacga atccgcgcgg gaccccatgc gccggcatga acaaagccat
     8821 aacggagcga gtcgttgggg cgcaacaggc ttgtatgggc tgatcgtcct gtctttcgcg
     8881 ttccgatccg tggccgcgga gcgcttgtcc ggtgctgatg agcatggcgt gtacggaggc
     8941 gggcggggga tccgggtcga cgacctgccg gcgtgcccgt tgcgcgcgaa actggaatcg
     9001 ctcgttcccg ccgcgcgggc gcgggcgctt cggaacctgg aagccgtttc gttccatcgt
     9061 aacgacctgg cctccctccg cgccgaccgc gaaggaggga tcttttatgc ctgcgccttg
     9121 gaatcgatcc gcgaagagcc gcccgggccg acccggccgc tccaggcgcg ggccgcgtcg
     9181 ctctccgtca ccaatccgcc ccttttccac agccgccccg ggtcgacgaa cgtgctgttc
     9241 ctcgacttca acgggcacgt gatcaccaac acgatgtgga acagcgacga gggtatcccg
     9301 cgatgggatt gccccccttt cgacgaggac agcgacgaaa ccacgttcag cgatttggag
     9361 cagcggcata tccgcgagat atgggagcgc gtggcggagg attttgcgcc cttcgatgtg
     9421 gacgtgacca ccgcgccgcc gcccgcctgg catttccgga cgggccacgc gctgatcacg
     9481 tccgcctacg atgccgaggg ccgcgagacg ccgcttgtct atccgggggg cacgtacggc
     9541 ggactcgcct acctgaatgc cttcacgaat tccgcgtatt cctacaacgc ggacaactgc
     9601 cggtcgcccg cgtggattcg gcagagcctg aacctggacc tctgcgtgca gatcgtggcg
     9661 gaaaccatct cccacgaact gggtcataac ctgggcttga gccacgatgg caccgcttcc
     9721 ggcgtcgaat accacgccgg cttcgccgcc acgaccaacg cgccgtcgtg ggggccgatc
     9781 atgggttcgg tggcgggccg ggacgtcacc cagtggagcc ggggtgagta ctctgatgcc
     9841 aaccagaagg aggacgacct cgcgatcatg gccctgcggc tcgggtatct cgccgacgat
     9901 cacggcgcga gtaacggcgc ggccacggcg atctcgccgg ccggctacgc cttttccgtc
     9961 accggccggg tcgagcagac gttggaccgc gatgtctttg cgctgaccac gggcgtgggc
    10021 atcgtcaccg cggcggtgta tcccttccgg gcgaccaact tgagctgggg cgcgaacgcg
    10081 gacctcgttc tgttgctctg cgcgtccaac gggacggtgc tggcctccaa caacgcgccc
    10141 gcggacgtga aggccgtgct cgcgccgcgg ccggtcgccg cggggaccta ttacctgcac
    10201 gtgtaccccg ccggcgccgg cttcccgtcc aacgcgtcga gcagcggcta cacggtctac
    10261 ggctgtctcg gcacctaccg gctcgccggc gccgtggccc cgccggcgga cacggtcgtc
    10321 gtgaccgcgc cgacccacgg cagccgttta ttcctttcca acgcgacgga gatcgcgtgg
    10381 ctgtccggtt tcacgaaccc ggtccgtgtc gagctatggc ggggcggggc gatgcacacg
    10441 atgatcgcga cgaacctcgc caaccaggga acccattcgt ggacggtccc tgcgtcggta
    10501 acgccggggt acggctaccg catgcgcgtg gcccgtgctg acagcccggc cgtgtgcggg
    10561 gaaagcgacg gccaggcggc ggtctatggc ttgcctgttt cccttttctt cgagaacttc
    10621 gacggatcgg gctcgttgcc ggcgggctgg aacgaggaat tcgtgaccaa tacggtttcg
    10681 tggcgcctgc agggcggcgg cggcgcgagc ggagggaaca gcccggccgc ggcgtatacc
    10741 gggtccaaca acgcccattt tttcagttcg ccccgggcgc agacgcggct ggtcctgccg
    10801 gcgctggaca tcggggacgt tctcgacccg cggctgtcct atcgcctctg catggcggac
    10861 atcgccatgg agcaactgcg ggtgctctac cggaccaatg cgctggcgga atgggcgccg
    10921 ctgaccaact actacggccg ctatttcact tcctggaccc agcactcgtt ccccttgagc
    10981 aacgcggcgc ccgattgcgc gctggccttc gagggacggg ccgcttccag ttcgtatgga
    11041 atctgcgtcg atgacgtcga agtgaccggc attgtcctgg ccggcgagct gttgaccaat
    11101 cgcctggagg tcgcgtccga tctcgggctg cccgacccgc ccgccgggac gaactggctc
    11161 tccgtcaatg cgccgcggac gatttccgcc gggacctcgc cgctgacggc gggctcgaca
    11221 cagttcgtgt gcgcgggctg gaccggcacg ggggacgtgc ccgcgtcggg cacggagacg
    11281 gcgctggcct tcacgctcta tgtccactcc tcggtcacct ggcggtggcg taccaactac
    11341 agcttcgact gctccgccgg agacgacggg acagtcgccg gcgcgaccag cggctggtac
    11401 gccgcgggcg ccttgatcac cgtgaccgcc gcgcccaacc cgtactgcga ttttacgggg
    11461 tggggcgggg acgcggcggg tgatacgaac gacctggcgc tgaccgtcac attggacagg
    11521 ccgcggcagc tggtcgcgaa ctttacgccg cgcctggcgg cccaccacgg cacgccggaa
    11581 tcctggctgg ccgagcatta tccagaggcc ggcgatctcg acgccgtgga gatcagcgac
    11641 acggacggcg atgggctgga ggcctggcag gaatacatcg cgggaacgtt gcccggcgac
    11701 gccctgtcgg tactggagtt ggagagcggc ggcgcggtga gcgagggcca atggctcctg
    11761 cgctggccgg cgatctcggg ccgttcgtat tccctgtggt ttttcaccaa cccgatctcg
    11821 cccgaggacg tgcccgtggt caccagccgc ctcgccggcg cggacggtgt catggcctgc
    11881 accaacgcca tcccaccgga gatcctgcgc ggtttctacc gcctcaccgt gcggcgcgaa
    11941 tgaaccgatc gccggccccc ccattcgcgg acaaggacgc ggccatccgc tggccctgcc
    12001 gattccgccc ggctgcaacg gccgcgacaa ggcgcggccc tcccgtttga gatatccagt
    12061 aacccgtgac aagggacgct ggagggcgcc gctccgtcgg cgccgtcagg ccggggcgcg
    12121 acgtgccgtc cacatcgcct gcaggatctt gtccgcgaat cggaggcccc gtttctcctt
    12181 gcattcgccc ggggtgttgg ggataaaacg gcgtggtcgc atcgacgcca tgaactgtcc
    12241 cgcgtgcaaa gcatccatgg ccatcctgga atatagcggg gtggagctgg atttctgttt
    12301 ttcctgcaag ggttgctggc tggacgccgg cgaactgggc ctgattctca ccggcgcgcc
    12361 cgaccggccc gacgacctga agctggaaaa ggcgtcgaag ggcaagcggc gctgcccgcg
    12421 ctgcgggtcg cgcatgctgg tgggcccggc gcccgccacc ggcgtggaag tggatgcctg
    12481 cccggccggc cacgggttct ggctcgatgc gggcgagctg gagcagatcg tccaggcgcg
    12541 cggggcgtcg gggcgggccg cggccgtggc gaaattttgc gcggatgttt ttggtaaaaa
    12601 ataacaggaa aaggagagcg ccatgggtct ctggatagtc atcggtgtga tcgtactggt
    12661 ggtcctcgcg tacatcggga tgtacaacag cctcgtctcc ctgcgcaacc aggtgaagaa
    12721 cgcctggtcg cagatcgacg tgcagctcaa gcgtcgccac gacctgatcc ccaacctgat
    12781 cgagacggtc aagggctacg tcaagcacga gcgggacacg ctcgagaaca tcaccaaggc
    12841 ccgcaccaac gccatgcagg ccagcggcgc ggccgcggcc ggcaaggcgg agggcgagtt
    12901 gagcggggcg atcagcaagc tgatgctggt cgtcgagaac taccccgagc tcaaggccaa
    12961 ccagaatttc ctcgcgctgc aggaggaact gtcctcgacg gagaacaagg tcggcttctc
    13021 gcggcagttc tacaacgacg ccgtgctgcg cttcaacacg aaggtcgaga ccttcccgtc
    13081 gaacatcgtc gccaacatgt tcggtttcaa gcaggccgag ttcttcgaga tccaggtcgc
    13141 gaccgagcgc gaggcgccga aggtgcagtt ctgatgatgt aggggcgtcg cttgcgacgc
    13201 ccgcggccgc gagcgcgccg ggcgccggcg agcggcgccc ttacgcctcc cctcatgtgg
    13261 gaaaccatcc agtcgaacaa gcggaagtcg gcggtgatga tcgtcctgct ggcgaccgtc
    13321 ctgatgttcg tcgggtacgc gctgggcgcg gcgctcgatc ccgccgcggg cgtgctcggc
    13381 gtgctcgcgg cgatggtcat ctgggtcgtg ctgacgctga ccgcgttcgc cggcggcgag
    13441 aagatgctgc tggcctcggc cggcgcgcgg gaggtgtcgc gcgagcaggc gccgcagctc
    13501 tacaacctgg tcgaggagat gaagatcgcc tccggcctgc ccgccatgcc gcggatcttc
    13561 atcatggatt ccgacgtgcc gaacgccttc gcggtggggc tcaagcccga gcgcgcggcg
    13621 gtcgccgtga cgacggggct catggcgcgg ctgaaccggg acgaactgca gggcgtgatc
    13681 gcgcacgaga tcgggcacat ctcgaaccgc gatacgctct tcatgaccct ggccggcgtc
    13741 atggtcggcg cgatcgtgat cctcgcggac gtgttccggc gcagcctgtg gtacggcggg
    13801 gggcgccgcc gctcgtcctc gtcgtcgaag ggcggccagg cgcaggccgt catcgcgatc
    13861 gtggcgatcg tggtggccat cctcgcgccg atcctggccc ggctgctcta cttcgcctgc
    13921 tcgcgcaagc gcgagtacct ggccgacgcc tcctccgccc agttcacgcg ctaccccgag
    13981 ggcctggcct cggcgctgga taaaatctcg cagttcaagc ccggcgcgct gaaggtgaac
    14041 caggcggtct cgccgatgtt catcatcaac cccctcgccg cgacgggggg cgggctctcc
    14101 ctgttcagca ctcacccgcc gacggcggat cgcatccgcg tcctgcgcgg gatgggcaag
    14161 agcgcctcgc tggccgcgta cgaggaggcc ttccgcgccg cgcacaaggg ttccggcgtg
    14221 atcgcatcgc gggacctggg cgcctcggtc gaggcggccg cccgggaggg cgcggcgggc
    14281 gccgccgacc cggccgcgca gtggcgttcc gcgcgcgacc tgctgcacca ggtcaatcgc
    14341 ttcctcgtgc tcccgtgcgc gtgcggcctg aagatcaagc tgccgcccga cttcaaggac
    14401 tcctccgtaa cctgcccgcg ctgcggcacg aaacacgagg tgcccgtggc ggaactggcc
    14461 gccgcctccg ccgccctcga ggcggccggc cggaaatgac cggtccacgc gccatcctga
    14521 tccgaggcgc ccgtctgctg gatcccgtcc ggaacctgga cgcgcccggc gaccttttcc
    14581 tcgccggcgg gcttatcgcc gcgccgcctc tgcgtatccc gccggacgcg gagatcatcg
    14641 aggcgcgggg actcgccgtc gcgccgggct tcatcgacct gcacgtgcac ctgcgcgagc
    14701 cgggcggcga ggaggccgag acgatcgaga ccggctgccg cgccgccgcg cgcggcgggt
    14761 tcaccaccgt cgtcgccatg ccgaacaccc gcccgccgct ggatacgccc gaactcgtct
    14821 cggcgcagat ccgccgggcg gaggaggccg ggctgtgccg ggtgcgcccg gcggcgtgca
    14881 tcacgagcgg ccggaccggc ggcgcgctcg cgccgctggc cgaactggcc gccgcgggcg
    14941 cctgcgcgtt cacggacgac ggcacgaccg tggcggacga ggcgctgatg cgccgcgcgc
    15001 tggaacaggc cgccgcgctg gggcgcccga tcctcgatca cgcgcaggac ccggcccgcg
    15061 agaaacaggg cgtcatgcac gagggggcct ggtccgcgcg ctggggcctg cccggcatcc
    15121 cgtcctcggc ggaaatcgag gtggtcgaac gcgacatccg gctggcgcgg gagaccggcg
    15181 gccgcgtgca tatccagcat gtatccgccg ccgggagcgc ggccctgatc cggcgcgcgc
    15241 gggccgcggg cctgccggtc agcggcgagg cgacgccgca ccacctggcc ctgaccgacg
    15301 ccgacgtgcg gccggaggac gcccgctgga agatgaaccc gccgctccgg tcggaagcgg
    15361 accgcgccgc ggtgctcggg gcggtggagg agggaacaat taccgtcctg gcgaccgatc
    15421 acgccccgca taccgcggcc gcgaaggcgc gcggattccg cgacgcgccg ttcggcgtta
    15481 tcgggctgga ggcggcggtc ggcgtgacgt acacgcggct ggtcgccggc ggccggatga
    15541 gcgcgctgga ctgggtacgc cgctggacgt ccggccccgc gcgcgtgctc ggcctgccgg
    15601 agccgtcgct ggcgcccggc ctgccggcgg acgtggcgct gctggacctg gatacggaat
    15661 gggaaatccg gccggagtcc ttcgcgtcga aatcccggaa cacgccgttc gagggctggc
    15721 gcgtgcgcgg ccgggcggtg cggacgttcc tgggaggccg cacgaccgcc cgcgcgtgag
    15781 ggcctcctgc cggcttgact ttcgtcgggg cggatgtttc cgtgtgcgct cgtgaacggg
    15841 gtgaaaagac cttccgtgtg gacgctggtt ctcggttgga accacgccga ggacacgatc
    15901 gaatgcctgc gcagcctcca ggccagcgag ggcgtggacc tgaaactttt cttcacggac
    15961 aacggcagcg cgccggacca ggtggaacgg gtctgccgcg aggtgccggc ggccacggtc
    16021 atccgccatc cgcagaacgt gggggtgccg cggggcttca acgcgggcat tgcctacgcg
    16081 ctccagcacg gcgcggatta cctcttcatg gccaacaacg acacgaccgt ggccccggac
    16141 acgatccgga tcctggtcga ggccgcggag cgggatcccc gcgcgggcat catcatgccg
    16201 cgcatcttct attatgacca ccgagacctg gtctggtcgg cgggttcccg cttccgccgc
    16261 ttcccgccgg cgatcgtcat gcgcaagggc cggggcgggt tcgagcggga ggaggacatc
    16321 gagttctcca gcctctgcac ggtgctgctg cgggggcgga tgttcaagga cgccggcctg
    16381 atggacccga acttcgtctt ctactacgag gactacgacc tctgcctccg catccgcgac
    16441 gcggggtacg cgatcaagct cgttcccgcg gcgcgctcgt ggcacaaggt ggcccggatc
    16501 acgcgcgccg gctcgaccag cccgacgttc tggcggaact acgggcggag cgtcgcgatc
    16561 ttccagcggc tgcagcggcg gcgccgctgg ctggccgggc cggccttcct ggccttccag
    16621 tacttccgcg cgctggtcga gggcaagcgg caggcgtggg cgccgttccg ggagggactg
    16681 cgcgagggcc gcgccctgga gctgaagccc gtgccgcgct gggatcagcc gccgatggat
    16741 cccgtcgaag tggcgcaggc cccctgacgc ggcggccggg ccgccgggaa aaacgccgtt
    16801 gcttcccgaa accgggtgtg tgatcatttt acccgccatg aagctcgtgg tgcaaatccc
    16861 ggccctgaac gaggaaaaga cgatccagga cgtcatccgc tccatcccgc ggaatatccc
    16921 cggcatccgg tctacgtcgg tcctcgtggt ggacgacggc tcgaccgacc gcaccagcgc
    16981 gctggccgcc gaggcgggcg cgatcgtggt ccggcacgac cgccggcggg gcgtcggcgc
    17041 ggcgttccgg accggcctcc agaaatccat ggaactcgac gccgacgtca tcgtcaccat
    17101 cgacgccgac gggcagttca atccggagga catccagaag ctgaccgagc ccatcctccg
    17161 gggcgaggcc gactacgtga ccgcgtcgcg gttcctggat ccgtcgctga ccccggagat
    17221 gccccgcatg aagcggtggg gcaacgactt catggcgcgc tggatcagct cgctggtggg
    17281 gcaggatttc aaggacgttt cctgcgggtt ccgggcctac gcgcgcgacg cctacctgcg
    17341 gctcgtgctg ctcggcgatt tcacctacac gcacgagacc tttctcacgc tggccttcgc
    17401 gcgcctccgg atccgggaag tgccgatcct cgtccgcggc gtccgggagc atggccagtc
    17461 gagggtggcc agcaacctgt ggcggtacgg ctggcgcacg gcggggatca tcctgcggac
    17521 ctaccgggac taccgccccc tacggttctt caactacgtg gccggcgtcc tgctggtggc
    17581 cgcgctcggg ttcctcggct tcctgatctc cgtgaaggtg cgcaccggca tgttcacgcc
    17641 gcacaagtgg tccggcttcc tcgccgccgc gctggccggg gccgccctgg tgctgttcct
    17701 gatcggcctg gtcgcggaaa tgctggaccg cctgcgcgtg gcgcaggatg aactggtctt
    17761 ccgggtccgg cgcatggaga tggacctgaa gcgcgcgaag cagcagccgt cgtaacgcgg
    17821 gaagccggcg catggccaag cccctcgtgc tgtcctactg gaacgtcaac cggccggtca
    17881 ccggtggcct gcggcgcgta accgccctgc tggaggccct cggccccgac cttgtcctgt
    17941 gccagcccga accggcccac cccgtgtacg agaccgcggc ctaccgcacg aactggacgc
    18001 gcgctggagg gggcggcatc aaccgcggcc tgttccattt cttcctgccc gaacccgcgc
    18061 gcgcggcgcg ggaagcctgg cggcgcgtcc ggcccgggct catcgtgcat acctccatct
    18121 ggaccttctg gccgctgcgg cgggcgggca cgccgatggt cctcgacgcg cacgatgcgc
    18181 tgggcgcggc catgaccggg cggtacgggc ggcgtcatcc gttcacgctc ctggtgcgcg
    18241 cctgggaacg ccgcgtggcg cggcgggtgg accgcctctt cgtctgctcg gacgtggacc
    18301 gggcctactt cgcgcgcgag tacggggtct ccgaggatcg gctctcggtc gtcccgaacg
    18361 gggtgcatgt ccgcccgccc gcggaggggc cgttgccggc cggttgggag gggcggctgg
    18421 ccggcaagac cgtgctcttc ttcatgggga agccggccta ccagccgaat gcccagggcc
    18481 tgcaattcct ccgcgacacg gtgctgccgg aactggagcg gcggcagccc ggccggttct
    18541 gcgtcgtggc ctgcggcggc gaggcgcggc ccggcatggc gcacccggcc cttcatttcg
    18601 cgggcgtggt ttccgacgag gagttgcagg ccttcatccg gcgcgcggac ctgtgccttt
    18661 cgcccacgtt ttcgggttcc ggcactcggc tgaaaatcct ggagtggctg gcggcgggca
    18721 aggccgtgat ttccacgccc aagggcgcgg agggcctggg cgcggttccg agccgggatc
    18781 tggtcgaggc cgaaggccgg gatttcgccg ccgccattct gtccctcgcg gaggcgccgg
    18841 atcgagtccg tgccctggga cgggcgggcg ccgacttcgt gcgccagcgg tacgactggt
    18901 cgtccagcag ccagccgctg tggcgggagg ggatgaggcg ttggctcgat ccacggcaga
    18961 ccggatgaac ggcgttcccg cgagtcggcc ccggcgggcg gcggccttcc tgctccggca
    19021 ggcgcctttg ctgctggtcg gcgccgtgat cctgtggggc gcgtgccgca ttcaaaccta
    19081 tatctgcgcc caggatccct ccacgtacat cgaccaggcc ctgcggttac tgcgggcgcc
    19141 ctggggctcg ccggagatgt ggcaggccct cacccgctat tcgccgctgc acaccctggt
    19201 gctggccggc agcatccggt gcttcggcac catggcgccc tactgggtga acgtgttctt
    19261 cgcgtgggct ttcctggcct gcctgtggtg gttcctggcg cggctctggc cgtccgcggg
    19321 ggatcgcgcc ctggcgctgg ccgtcgcggt gacctgtctg ctggccggcc atgacgcgaa
    19381 tatccagttt ctggtgtatc ccttccggga ggcgtcggcc ttctttttca tcttcctgtc
    19441 ctacgggctg ctgctgaagg gggacgcgga ggaccggcgg agtttcagcg cgctggccgg
    19501 cctggcgatg ctgctcggcg tggccgcccg cgagcccttt gtcatggccg tcccgggccc
    19561 gctcctcctg gccgtgacgg gccgcggccg cccctgggcg cgacgcctgg cccatgccgg
    19621 cctgttcctg ctgccgttcc tgctcgccgg cggcgcgtgg tttaccgtca cggcgatgac
    19681 cgggcagggc acgaccgccc agttcaagga ctgggccgag ctgatgcaat cgcacggcgc
    19741 cggaaccttc gcggcgcgca cgctcggcaa cgtccggtac ctggccgggg tcattctcgg
    19801 cgagatcggg tggtcgggcg tcgccctgct ggcggcgggg ctctacccgg ggcgtcgcgc
    19861 ccggatcgtc tacctgctgc tggtcgtgcc ggctcttacg gtcggcctct tttacagcac
    19921 gtacaacatc tacatgcgct acgtgatgtc cgtgctgctg ttcctcgggc cctgggccgg
    19981 gaccgggctg ctcaacctgc tggcgctcgc gcggttcctg gcccgcggga cgcgcctgcg
    20041 gctggcctgg gcgccgctcg cggggagcct cctgctgctg ggctccctcg cggtgcaact
    20101 ggcgagcctg ccggccagcc ccttcacgcg gcgggaggtc ggcgcgttcc tcggccggct
    20161 ttcaggcctg ggcaccggaa ccgtctacta cgtggagcgg atgaaccggg acatctgggc
    20221 cgcgcttcgc aatcataccc acctcgaaac cagccgcgcc ctcgatgtcc cccgaaacct
    20281 ggaagccggg cggccgatgg cgtttctgaa gccgctcaac gccgccagcc gtgcccgcgc
    20341 caaaggacgc gaggacggag ttcggattga ggaactgctt ctgcaatcgg gcgacctggt
    20401 tccgtggccg ggcgtggtcg agccgtggcg cctgggccgg ggtgcatatg ccgtcctgca
    20461 ggtcgtgccg cgaagccgga cgtcggtgag cgagccggtg gccatccccg ccggaacggc
    20521 cggcgtcctc tggctgaact tccgggatgt ccggccggaa gccgtgaaga aaatccgcct
    20581 gctcggaccc gatggccgtg aacgcctggc cacggagatg atggggcagg gcttgcagcc
    20641 ggtgtggtgg cccggcgatc cggccgagga aaccaatctg gttctcgaga cggcctcgtc
    20701 ggcgccgctg cccgcgcggg tgtacaccgg attccagcct gcggcggagg cccgtgagtt
    20761 tcccctggac gcccggcgcc tgctttcggc gacgcggtgg ttcgagccgc cgttcggcca
    20821 ggcgccggcg cgcttcagcc cggccgtatt tttttccgag gggggccgat tgcggctgcc
    20881 ctggccgaac gatccccgcg cggaccggct gcaactgtcc ctcgagggcc gcgtgccgcc
    20941 cgaccgggaa aagacgggac agctcaccgt gcggtcgggt ccccgggaag tgccctgcga
    21001 agcgaaccgg gtgggctacc gctcctggaa cattgacctg acgatcgaga gggagtccgg
    21061 cgcccgcgag gccgtgctgg aactgtctta cgagccggtg aacacgaaca ggccgctcca
    21121 tctcaccgtg agccaggcgg ccttgcgcta cttcggctcg gaatagaagc cgacgcggtc
    21181 cacgggaatg cccagttgcc ggctgtcgcc gacgcccagg agttcgtgcg gcgaccaggt
    21241 cgtactggac acggtcagcg tgttggtgcc cggctgcacc agggtcgccg ggatggccgc
    21301 ctcaacgcgg ttggaatcca ccaggtgggc cgccaggtcc gtcccgttga acgacagcgc
    21361 gatctccgcc ggcggcgcgt tggtcgcgcg gatctgcggc ttgaaatcca cgcgcacgac
    21421 ccggtccgtg ccgtccgccg ccagccagaa cgccagcacg cccttcccgt cggtccatcg
    21481 catggtgtcc tccccgcggc cctcgcgggc atagaaaccc gagacgatgt gggggaagtc
    21541 cacgccgctg ccgatatcca ccgaccggat ggcggacaga tcatggacgg tgatgcgatc
    21601 cagttccggg gggaagggcg gctcgggcgc gccggacatg acatcgagcg tttccagccc
    21661 gatgcggcac acctggccct cgcgcgcgcc ggcggggatg cgcagcacgc ggcggtgaaa
    21721 gatgtagctc cgcaggatcc gccgggtctc cggcggctgg ccgtcgggcg taaagcgcac
    21781 gtcgaccggc acctcgaggc tctccttggt cgtggggcgc acgcggagat agaaggatac
    21841 ctggatgaga tcgttcgggg ggtagggcat gggcacggcc acggtcgcgc gggccggcca
    21901 gtgcggcttg cggcgcgtct ggcccttgag gatcgtgaag ccctcttcca gcacggcgtc
    21961 gtgcaccgtg tcgccgctgc tggcgaagcg cagcacgagc ggctcgtcgc gccggacgag
    22021 tccggccccg aacgtgttgg gaaccggctg gtcggattcg agcgtcaggc ggtacggagg
    22081 ggtaaaggac ggccagtcca ctcggtagta ttgcgtgccg ttgccgacgc gcggcgcgat
    22141 gacccggccg ttcaggctca atctcgccca acggcgcggc acgccttcca gccagagcgt
    22201 cgcgccgttg aacaggagga tgtcgtcggc gtccggggcg gcctccagcg tgaggctcgt
    22261 cgcggtattg gtccagagcg aaaggcgcga gagcgcgagg tcgcccagcc cgacggccgg
    22321 ctggaagtgg tagcggtccg ccggggcccg ggccatggtt tccgtgtcga acagctgccg
    22381 gacgatccgg ccgaagggat cgtccgtcga atccatgaga tacaggggat gcccggcggc
    22441 cagccgctct cggacatgct gatgaatttc cacctccagg ttcgtgggat tcaggatcgg
    22501 aatcgcgtag gcctcgagcc gggtgaagta ggacaccagc ccgccgaggc cgggcggggt
    22561 caggatgacg gcgtcccggg gcacggcctt ttcgatatcc gctttcagcc gccgcgcctg
    22621 cgggatctgg aacggcggca tctccggcgg gcgcaggagc ggaatcaccg cgaccaggac
    22681 gaagagggcc gccaggctgc ggagggccgg aaggatccat cgctgcttgc cggcggcgca
    22741 caggaggcgt tccgccaggg ccagcgcgcc gcaggcggcc aggggcaccg accacagcac
    22801 gacgatgaag aagtaccgct tcacgaagac ccagtagaag caatagaaga tgaaatggac
    22861 cagcgcggcg ggcagcagca gatcccgcag gaggcttcgt cgccgcacaa cggccaggcc
    22921 gatgccatag accagcccgg ccgcgtacag ccatccgccc cgccaccaga aatagcggag
    22981 ggcgtggatg agcgtgggaa caaaggcccg aagatggagt ccgggcagca ggcggccctc
    23041 gaccacggcc tgtggcggca ccacggcatg cccggtgcgg aagtacgatt gcagcaccag
    23101 cggcagcacg cccagcgcca ggaacgcgcc gaacaggatc accgagcggc cgagcgggat
    23161 ggcgcggttc ttccgccacg ccgacaggcc gtaccagccc atcacggggg ccatcagcac
    23221 cgcggtctcc cggacgtccg tggccaggcc cagcgccagc gccgacagca ccagggcgat
    23281 ccagggccgg ccgccccggc ccaggtaggc gatcagcaga aggacggatg tcagcagcag
    23341 gacatgcgag aggggatcac gataaggatt ggtcaggtag accaggattt cggaatcgaa
    23401 gtaaagcaac ccggccgcgg cggcgacgcc ggcgtacgcg gccgcgcgcg cgtccatgca
    23461 ttcccgggcc gcgaggacgg tcagccgggc gaccaggagc acgatcgcga cgagcagcgg
    23521 caggttcgag acgaacacgt agaatggccc gaccagtcga atcacgaagc ggaggaagac
    23581 cgggtaggcc agggggaacc gctggaggtg gtcggtcggg tggaggttgt gggccagcgt
    23641 cagccagttc aggggatcgg agtacggctt caggtagtag tagttccaga cgaaccacgc
    23701 caggccggcg accacgaagg cgatcagcgc gccgggccgg gtaagggcgg acagaacggc
    23761 ggcgagccgc cgcgcgccgg gcccggacag gatcttgtgg cgccacggat ggagcatgcg
    23821 cacccgatgc ctcactccac gggcgcctgc ggctcgacct ccgagcgggc gctctcgggc
    23881 ttggccatgc gctcgtacat ggcttcccag tacttgttga gctgcagggc ctcgttgcgg
    23941 gggatgtagg cggcgtcgac caggccctcg ttgagcaggt agtggaaaat cgcctcggcg
    24001 gcgatccggt gcgcaatctc gctcggatgc ccgtccgcgc cgggcagggt ttccatccgg
    24061 gtcggcgaac ggccccggag cgaatccagc aggtccagat agcggatgtt ctcggactgg
    24121 aggtgggcga tgatcttgtc gtgtgcgaac tggaaggggt agggggtccg gtccacgaag
    24181 gacaggcagg gccagatcac cacgaggaat tcaacctgct ggtcgcggca ggccttgtgg
    24241 aagtcgcgcg cggcgtcctg gaacagcttc cagccgctgt agtcgtcgtc gtacagccgt
    24301 tcgtagtagc gatggaagcc ccgcccgctc ttcacgtcgc cgatcttctt gttgaaccac
    24361 gccagggccc gcgagcggcg gatgagcggg gccagccagc cggtgggcac ggggggcatc
    24421 atctccgtcc gccaggcgcg cagttccttg tagttgttga aattttccgt gtcgttcagg
    24481 tacatggcca ggatcacgag gcgcggccgc gtcgccaggg cctcgtccag gaacccgcgc
    24541 tgcatgtagg tcgaggtccc gcccttggcg aagacgtcca cggcggccgg cgtggcgtcc
    24601 ttcttgaggt tgagcagtcg ctccagccgc atgccgaagg tgtcgtccgg ctgcacgccc
    24661 tggccggtcg tgaacgagtc gcccacgatg gcgacgcgga gcacgtggtt ggtgtcccgc
    24721 acccagggat ggatccgttc ggcgtcggaa gcgaagtaga accggctgcg gtcgaagagc
    24781 ggggtctgct tgatggactc cggcatcggg ccgcgcaggc gcagcgcgag ttccagcgcc
    24841 gcgagcgcgc agaacagtac aaacagcatg aggacgacgc gcaggatgat ggatttgacg
    24901 atgggcttca tattttcttg attacatccg attgtaaggg cgggggcggc gcgccgcaac
    24961 gtcattccgg ggtcaggggg gcgggctcgg caaccggctc gccgtgtccg gccagcctgt
    25021 ccgcctcgtc ctgcttgacc gcggtcacat cgttcatgaa ggccgacagg atgcgccact
    25081 gctcctccgg agaccggctg gcgattgccg gcaggtattc gggatagatc agcttttcgg
    25141 ccagcaggta cttcaggatc gcctccgcgg cgatgcggtg cccgatctcg ttcgggtggg
    25201 catccacccg cgggatggct tgcagccgtt ccgacggcag ccccttgtag acggggagca
    25261 aatccagata cggtattccg gcggcatcga gttcctcgtg gacctgctca tggatccagt
    25321 cgaacggata atcatcgatg tggctaagga gcggaaagac cacggccacc atcttgatgt
    25381 tgttccggcg cgtggtctcg gagaaatcct tgagcgcctt ggtgaaaagc ttccagccgc
    25441 cgtactcctt gtcgaacagc ttgcggttca tttccaggta gccgcggcgg gcgcgggcat
    25501 cctccagttt ctggtataca agcgtggcca ggcgcgacca gcccagcgaa cgagcgaacc
    25561 agcgggaggg ttgatggggc agggcatcca tgcgccatcg cgtcagttcc tccttgcgcc
    25621 gggggtcctc ggtatcgttc aggcagatgc cgaggatcag cagttccggc ttccatgcga
    25681 cggcttcctc caggaatttc aactcgccga aggtgcacat gccgccccgc gcccagacac
    25741 ggacctcggc gggccgttga ccgtcgttga ggttcaggag gcgctcgagc cgcgccccgt
    25801 aggcatcctg gggctggctt ccggcgccca cggtgatgga gtcgcccagc acggcgatgc
    25861 gcagcgcgtt cgtcgcgccg cggctccacg ggtgcatcct ggtctcgccg tcgtaaaagg
    25921 aaacgggggg gcgatcatga cgcagttgct ggtcgggcgc cacgcggtag taggcgcgca
    25981 ggcccagttc gcagaaggcc aagatcatca ataacagaat tcccgagaac acgatctttt
    26041 tcacgctggt ccgtccggtg tgagcatggg gcgattttgt agcgcgtcgg cgggcatttc
    26101 acaagagcga acacccgcaa ccttgccgat acttctgatg agtctgcgcc ggcccggtcc
    26161 acggatgggc tggaacctct tatgtatcct gtagatgaaa agcggaggcc ggtcagcatc
    26221 gccatgctca cacttctaga agtgtgagca tcagtccgga taggcaatta tgagaactgc
    26281 gtcacaagaa tttgcattac agcatatagt gtcgatttat attagcatca cacttctaga
    26341 agtgtgagca caagtttact gacgtttttt tacaggaacg atcctcactg gagaccgagc
    26401 cgtgccatcg ccgcctgttt ccatcccaaa aggaggccgg tcggcatagg cgcgatgcgt
    26461 tggcggcatg ccctgtcgga tgccggtccg tgacgacgtc aacgttcccg acgattcccc
    26521 gaccagtatt ggcgatcgga tccgcgggtg aatgccgggg ccgggactcc cgtgggcggc
    26581 cgccggtcgc gacgcgcggc ttaggtcgct ttcccgccgg cgagatcctg gaacacggtc
    26641 tcgtaccgcg ccgaaatgac ctcccacgag aactggtcgc gcgcccgctg ccggccggct
    26701 tcgcccaggc gccggcgctc ggtgtcgttc cggagaagag ccgccaccgc agcccgcatg
    26761 gcctcggcat catccggctc caccaaccgg cccgtgacac cgtcctccac ggcctccagg
    26821 attccgccga cacgcgtggc gacggacggc aaccccgcca gggccgcctc gaggaacacg
    26881 atcccgaagc cctccacgtc tcccgggatg tcccgggccg gcagtgcgaa gacccttgcc
    26941 tcacggtaca gggccgccac ttcctcgtcg cggagccggc cggcgagatg cacgtggcgg
    27001 gccagcccca gctcctgcac gcgccgcgcg atcaggtcgc gcctccgctc atggtgcaca
    27061 agggaatgat gagcgtcgtc cccgaccacg acgtacaaca cgtcggaaaa ttcccggacc
    27121 agcgggggca tcacctgctc cacgaactcg aggaccccct tgcgccggat gagccgaccg
    27181 acggtcatca gcacgcggcg cccggccacg aggtccgaca attctttcgg cagcggcggc
    27241 ggggagacgt cggcgggcgg cacgaggacg ccaggcggaa cgacaacgat ccgctccggc
    27301 cgctgcccgg ccgcgatcag caggtcgcgc gtccgcccgc tgttcgcgca cagccggtcg
    27361 gcccggccga acagccagcg gacggcgcgg gtgtacaacc atccggggta gaggatgtcg
    27421 ctgccgtgcg ccagcacgac cagcggccgg cggaacagcc gcgagagcat ccatcccgcc
    27481 ggcgcggaca cgacgctcga gcatgcgatc acgtccggcc gttccccgcg gcagagcgaa
    27541 aaaccccggg caagggaaaa caagacatag gctttcagac ccggccgcgc cgcgcgccag
    27601 gtgtccggtt ccggcggtgc gtccttcgca tgggccgtga ccacgcggac ggcgtggccc
    27661 ctcgcccgca atccgcggac gagatgttcg gcgacgtact cgatcccgcc cgtgaccggc
    27721 gggtagttcc aggtgagcat caggatgcgc aggcccatgt cattccgttt cgttgcggcc
    27781 ggccgcggcc ggctcaatcg ccgcttggac cgtttcggtg gcaaaccata gcgttgtccc
    27841 gccgggcgca tcaataaaaa ggtcgagcgg cctggccgcc tgctggggat ccaatatcgc
    27901 gcccgtccaa atcctggatt ggggcagggc gaccgcccag ttcgtcacgg cccggccatc
    27961 ctgctcgtac cggaaggaca ccgttccctg gaagggggcc gccttcatgc cgagggccag
    28021 gttgaacgcg ccgtaggatc cgtgcacggc cggcaggcgg agccgtccgc ccgacgaagc
    28081 gggcgcggcc agcgcggcgc cgacgaacgc cggctcaaac cagcgctcca gggacgggcc
    28141 gcgctggggg ccggcttcga accgcgccgg cttgccggcc gggaccaccg tgtacacgaa
    28201 gccggaggga cccggtcggt cgcccgatac gtcgacgagg gccgccccgg gctccgccgg
    28261 cttctcgaag atgaaggcct gcagaaaccg cgcatcctcg acgagccacg aagccagtgt
    28321 ttgtcccccg tccgtccgga cctcgacgcg ccgcgcggca ttcgacgggc catccttgaa
    28381 atccagccag agcacggccg tgtccccggc gggcagggtc acgcgcgcgg agacgctcgt
    28441 ggcggaccag ggccgcacgt cgtacacgcc ataccggccg ccgccgaggg ccagctggag
    28501 cggttggccg tccgggccca cccgcgcctg gacgtcatgc cggtaacgaa ggtggcgcga
    28561 cgccgagttg gccaggtccc ggcgaaagga gccgggcggg aagcagtcgc gcgtcagggg
    28621 ctccaggtag aaacaccggg cgccgccccc gtccatcccc gcgcgcgcgg gatcggcggg
    28681 ccggtgcgcc aggtgataga tcatcgtctc gccgagatgc cggcagatcg gctcgatcac
    28741 cacgagatcc tccggccgca cgaccgcatc gaagtcccgg cggaattgct ccacgtcctg
    28801 acgcgttatc cgcgggcccc acggctgaag ggtccaggcc gtgcggatga gcccgacggc
    28861 cagcagggcg gcggccagga cgcgcgcggg tccggcgatg ggacggcgga ccagccggac
    28921 agccagaacg gacaatttat cgatggccag gtgaaggccg tacgcggcga acgggcagag
    28981 cggcagcagc acggccaggg cgtaccggcg gtgcgccgtg taggtcgcgt acaggaagag
    29041 ataagccagg gcgctgaaca ggaagaacca gagcgccgcg cggttgcgcc gcaacccggc
    29101 gaggcccagc aagccgagca gcagccacgg atagcccagc gcgtcccgga gaaagacgag
    29161 cagggcgcgc agggaatgga cgtaggcgtc ccggaaggcc gggggcggcg tgtgcagcag
    29221 ccgcgcgctc caccactgcc attgcccgct ggacggggag cccacggcct gccgcgccag
    29281 gagccaggtc ccgccggcca gcagccacgg caggatcgcg ccgaaaaagc acgcccggcg
    29341 cgcgcgcccg ctttccttcc gccgaaagag cagccacagg tacgggccga tggccccgca
    29401 aaccgccggc tcccgcatgg cggccgcgat gacgaacgcg caccctccgg cgaaaaacag
    29461 gcccggcttc cgccggtcgt ccgccaggag aaaaaggagc caccccgcgg ccacgaacag
    29521 gaacgacggc gtctcgcgaa acgcgtacag gagaaagtgc gcgttcagga catacccgcg
    29581 caggaggatc agcagcgtcg cgagcaggcc cagcccggcg gcccggtcgt ccccgaaaac
    29641 gcggcggaga accaggagat acaggcccag caggaggccg gcgagcgcga cgttgatcca
    29701 ggcggtgacc cacggcccgg ccacccggat cgccagcgcc aggaacagcg gccatcccgg
    29761 cgcgacatgc gtaagcgcct ggcggaattc cggggaggac cggtcggcgg acaggatcgt
    29821 gcgcgcgaaa atcctgtgcg tggcgtcgtc ctggccgctg accgccgtcc ggatctgcca
    29881 agcggccagg gccaggatgc ccagggcgaa cagccaagcc gccaggggcg ccaggcgggg
    29941 cgccggcaca tgaggtttgg ggtccatgtt gctgcggcaa atcgtataac tccgcgaccc
    30001 tgcccgtaaa gcatcaaggt ccggccggac cgcctcgctt ttttttgtgt gccgggagta
    30061 aacttggcgt ataaagaagt gtaggttgca atcatgaggc gctcctgtca ggccggcggc
    30121 ataacctgcg cgttgcttgc ggcgggctgg ctggcgacgg cctggtccgg cgaggtcccg
    30181 cgcgtcgagg tcgggggcgc gaccgtgtcg gaggaaacgc ccgccgtgcg gattgtccgg
    30241 aaaatcgcga ggccggccgc cgtcggccgc cccgtggatg tcgtggtcag cagcggcgaa
    30301 tccccgcaga cgaacgggtg ggatgtggcg gacggcgacc tcgagaccgc gtgggcgggc
    30361 cgcgccggcg aaaacggctg gtggctggcg ctggtctacg agccccctct gaagatgacg
    30421 gacatcgaac tgctcctggc gccttcctcg acgaccaacg tcatgatcct cggcagcgcg
    30481 gatgcgctgg actggatcga actgccggcg gccccggagt cgggcctgga ccggatccgc
    30541 tatctctgga tcgtcttcga gccggatgcc tccggacgcc ggcccgtggt cctggaagtc
    30601 aaacccatcc cgtagccgcc ggcgcgcccg gcgggaccca tccggcggga ggaccggaca
    30661 ggcctatgcg agtaacggct gtgctcaagg aatgcgtgcg gcaggaagtg gaggcgcgcg
    30721 cctccccgtc gcggcaacgc cgtctccagt ccgcgcggct ggcgcgcctg gtggcccgcg
    30781 ccgccgtgcg cgtgccgttc tatcgcgagg ccttcgccgc cgcctcgctc gacccggcga
    30841 tcgtgcgagg tttggacgac ctggcgcgga ttcccgtcac ccgcaagctg gaataccgcc
    30901 gctgcccgcc cggggagcgg ttggccgcgg gcgtgcgtcc cgaggactgc cgcgtggagc
    30961 acacgagcgg gtccacgggg atcccgctga acgtctacct ctcggaaggc gagcaagccc
    31021 agcgctcggc gctctggctg cgcgcgcggc tgcggtgcgg attgtggccc tggcggcggc
    31081 acctcctggt cggctattac cagcccccgc ccgtgccgtg gttcctgcgc cggagacgga
    31141 tccacgcgac ggccgccgag gcctgcgcga tcctcgggca tttccggccc caggtaatcg
    31201 gcgcctatac ctcgcagctg ctcctcctgg ccgtggaact ccagcgccgc ggcattccgc
    31261 cgccgcggcc ccgggtgctg cgcacgggcg gcgagacgct gtccccggcg gcgcggcaac
    31321 ggctggaaca gttcttcggg gcgccggcgc gggattttta cgcggccgtc gagttcggcc
    31381 tcatcggctc ctcctgcccg gcgcggggcg gctaccatct tgcctcgggc gacctgctgg
    31441 tggaaatcgt gcgggacggc cgcccggcgg cgccgggcga ggagggggaa gtcctggtca
    31501 cctcgctcgt ggcgcacgcc atgccgctga tccggtacga aatcggcgac gtcggcgcgc
    31561 tcgcgcccct ggagtcctgt ccctgcggaa atccctttcc gcgcctggcc ggcctgtcgg
    31621 gccgctcgga ggatatgatc gtgctgccgg gcggccgccg cgcctcgccg cgcctggcgg
    31681 ccatcccgtt ctgggaaaac accagcatcc tccagtaccg cgtgacccag cgcgccgtgg
    31741 gcgagttcga ggtggaggtg gtgcccgacg gaccgctcga ccccgggctg gccgaccgcg
    31801 tgcggagcta ttttcgcgag cacttcgagg ccgcgcgcac ggacatccgc gtgcgcgacc
    31861 ggatcgatcc cgatccgcag ggcaagctgc ggaaggtcgt cgtgctggcg tcagacggcc
    31921 ggtgatcccg cgcggaaacg gtcccagtaa accgacgggt cctgcgcgac gccgttgacc
    31981 cgcaattcga cgtggaggtg cgggtagtcg tagtcgccca gcccgcgcgg gatgcccgtg
    32041 cggccggagt agccgatgat ctcccccatg gccacggggc cgcgccggcg gacgatccgg
    32101 tcgaggtggg cgtacaggct gaccgtgttg gtgaaatgcc ggatgcgcac ggcccgcccg
    32161 tacgcgccat tccagcccgc ccgccggatc tcgccgtccg ccatcgcgcg gaccggcgtg
    32221 ccgggctcgg ccggcagatc gatgccttcg tggcgccggt cggcgatccg gtacgcgccg
    32281 aatgcgccca ccgcccgcgg cgcgggcatg gcctcgatcg gaggggcgaa ccacggccgg
    32341 ccgaggcgcc ggatgacgtc gccgagcgcg agctggttgg accccttgaa cagcgcgacg
    32401 tcgccgggcc ggagttcatc ttccagccgg gcgagcagct cgaacttgct gtccgtctgc
    32461 gccgtgcgct ccgccgggaa gcccgcctca acggcgccct gaaggatgaa ctccgagagc
    32521 ggcgcgtacc cgaacagccg ctggatgccg gggaacgcgc gggccagccg gcgcccgagt
    32581 tcgcggtgca ggtccgggcc gcgctcgccc agctcgagca tgctgccgag caccacgacc
    32641 ttgcggccgt ccagcaggtg catcgcctcc agcgccgccc atgcggcctc ggggttggag
    32701 ctgtaggtgt cgtcgaggac cgtccactcg cggttcgttc cgacgctcac gcgggacggc
    32761 gccggccgga accgggccag cccgcgccgc atcgcctcgg cgtccaggcc gaagagcgac
    32821 gcgcaggagg cggccgcgag caggggatac acggcgtggc gacccagcag ttccgattcg
    32881 atccgcgcca ggacgcggcc ttcacggacc aggtcgaacc gcacgccgcg cgcgtcggcc
    32941 tccacgttcc gcgcccggaa atccgcctcc tgttcaacgc cgaacgtctt cgcccgcgga
    33001 tcgctccgca gcgtccccag caggtcgtcg tcggcattga cgaggagcgt gccgccgggt
    33061 ttgagcgcgc cgaggagcgc cttcttctgt tcgaacacgc cctcgcgact gtgcagccat
    33121 tgcaggtggg tatggccgat catcgtgagg atcagcacgt ccgaggcgac ggcggcgttc
    33181 cagcgcgggg cgttggaggc gctttggatg ccggcctcca ggaccaggat ctgccggcgc
    33241 cgccgggcgc cgagccggaa gacgaaccgg tgctggtggc agtcgatatt gtcgttgtcc
    33301 cgcgtgccca ccgtctcgca tcgctccgcg agcaggctct gcagcagcag gcacgtcgtc
    33361 gtcttgccgc tgctgccgct gatgagaatg acggggacat cggagcggta cgccagccag
    33421 gcgcgcgtcg ccgcgcgcag cagcggcccg ccgcccggaa tccagccgga gacgaggccg
    33481 gcggcttttc tcgcgcccgc ccgccaaccg gttcccgcgc cgttcatggc ccgctccctg
    33541 cgccggcggc gcggcgcgat aaggatcctg accgggcgtc gcggaccagg tcggcatagc
    33601 cgctctggca ctccgccgcc aggggcgccc agcgcgcggc ctgctccgac cggatttcct
    33661 gcccatgccc gtgaagccgc gccagcttcc cgagcaggag atccccgccg ccgggcccgg
    33721 cggccgcggc gatgtccacg agctgttctt cgagaccgag ccgctcgaac aggttgctgc
    33781 tcttgcggac gtacccgatc cccagggtcg ggacgttcat gccgagggcc aggatggcgc
    33841 tgtggaggcg caccccgacg aaaagatcgc acaggccggt gagcgccttg atccgccggg
    33901 ccgggatgtc caggttcagg atatgcgagg gcaggcgaag ccgccgcccc aggtccagcg
    33961 attccaggtc gtcgccctcc caggggacgg cgtacatcgg gaaaaaaagc aggcggagcc
    34021 gccggccctc ggaaaaccgg ccgagggcgc cggccaggtt gtgcagcaac tgctcgtggg
    34081 tgggcggggc ctccggccgc cggccggtca gtctccattt ccactggagc ggaaagaaac
    34141 ggcgaccgat gtggaaccag cgccgcagcc cgatcccgat ccggacctcg tccggccgaa
    34201 tcgggacgcc ctggtgctcc agttccgcgc gcgcctcgga ttcggccgcg ggctggagcc
    34261 ccacggcagg atccggcagg acccggatcg gcggcggggg
//
LOCUS       DAOOTK010000024        32904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_981_length_32904_cov_6.485068, whole genome shotgun sequence.
ACCESSION   DAOOTK010000024 DAOOTK010000000
VERSION     DAOOTK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 32904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32904
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_981_length_32904_cov_6.485068"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK634_04655"
     CDS             <1..282
                     /locus_tag="PK634_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97060.1"
                     /translation="WKAGLSRLLRTAIAISLVFATTGMNLQTSGKCPGGGGDITEVFT
                     TFAVMTYLPLAGVLLVDLIARLIFKWEDKKDSEPATPPYSEPAARSPQG"
     gene            329..742
                     /locus_tag="PK634_04660"
     CDS             329..742
                     /locus_tag="PK634_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97061.1"
                     /translation="MRTLALAVVMALIGSIAVFAEDIAIKAFDVEGRISFDEVADAWM
                     YQVNWTTNLGETTWRTSSPPGVARFAAQGDETRVVTVGLQNASCFFRIEATNYPPATR
                     RNGPDSSRNLRDGLRDQSSPRTKRERGNYRNTASA"
     gene            1947..2351
                     /locus_tag="PK634_04665"
     CDS             1947..2351
                     /locus_tag="PK634_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HOW97062.1"
                     /translation="MIKGGCYCGAVRYEVRGRLLLFANCHCPDCRKFTGSAFASVLVT
                     ETQGFEVASGEEQLVPFQSSPGKRRFFCRHCGCHLFSRAEQRPGLVFVRAGSLDDDPP
                     MRPQCHIWTSAKAPWHEISDSLPQHREGLPRN"
     gene            2711..3250
                     /locus_tag="PK634_04670"
     CDS             2711..3250
                     /locus_tag="PK634_04670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOW97063.1"
                     /translation="MKLEAASGRAPPGLLELLRDLGDGENGFMGTPVPSGEMGLEEYL
                     QRCVEGPDPARLRPGLAPQTVFWMIDDDGVAVGMVRMRHSLNDRLRAHGGHIGFYVRR
                     DRRGRGHARAALRMALAELAKRGERKALLTVATDNAPSLRVVEACGGRCDGESVDPES
                     GRRLRRYWIDLPPPAGRPE"
     gene            3264..3833
                     /locus_tag="PK634_04675"
     CDS             3264..3833
                     /locus_tag="PK634_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97064.1"
                     /translation="MRKALFPVMLLAALGYGAVGETPAPVAPDQVVLLDVQGLWGGVD
                     LWIAKDGRAVCRLVGPPKAGGRGLQESRYAFALSAEQQAELAALVDGHGFFTLQTADR
                     QGVPDESRPAIFIRTGARRRAVAKWGNDAHPDFDPLYRFLLAAARAGRTGTLLAEGAY
                     EPDWQPDGFETGRAIRDMTRTPADPPPAE"
     gene            3912..4316
                     /locus_tag="PK634_04680"
     CDS             3912..4316
                     /locus_tag="PK634_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HOW97065.1"
                     /translation="MIKGGCHCGAVRYESDGRVLRFVNCHCPDCRKSSGSAFSSVLAV
                     EAGGFRVVAGEDRVVPYESSPGKFRCFCRTCGSHVFARAAQRPGMILIRAGTIDGDPG
                     VRPEVHIWVGEKAPWHEIGDSLPRHTEGLPPK"
     gene            4313..4765
                     /locus_tag="PK634_04685"
     CDS             4313..4765
                     /locus_tag="PK634_04685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOW97066.1"
                     /translation="MSRLPDPGGPVYCEEFPVPPQAVDVNGHVNNVVYVQWMQDMAVH
                     HSDAVGGTRAMSEAGGRWVVRAHRVEYLAPAFEGDRIRVATWVADFRRVRSLRRYRFE
                     RASDGLLLARGETDWVFVDAATGRPRTVPDDLRRRFPLHAAAGPEAGE"
     gene            4783..5172
                     /locus_tag="PK634_04690"
     CDS             4783..5172
                     /locus_tag="PK634_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOW97067.1"
                     /translation="MDAAARERVLAELHRDVALRQQGYRERALKLFPHVCARCGRAFE
                     GRRLRELTVHHRDHNHDNNPPDGSNWELLCLYCHDHEHEGNLMAGLGAQAGGEGAAPG
                     TFSPFENLDSLLKNGAPPEAGRPGEKP"
     gene            5169..5666
                     /locus_tag="PK634_04695"
     CDS             5169..5666
                     /locus_tag="PK634_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97068.1"
                     /translation="MKKGLAVLLMLAAAARAEERFMLSDPEVGAEYGPFVYEDGAILE
                     VYGRPLTLRRLDTKADALKQKMEQITLPSIEFRNAALPDVVQFLVDASVAGDPEGTGV
                     NIVLPPEPAAGEAAPRTVTLSLRRVSLYDALALVAEVSGLSVRLDERGVVLLEAGGRA
                     GGASP"
     gene            5680..6050
                     /locus_tag="PK634_04700"
                     /pseudo
     CDS             5680..6050
                     /locus_tag="PK634_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
     gene            6047..7717
                     /locus_tag="PK634_04705"
     CDS             6047..7717
                     /locus_tag="PK634_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97069.1"
                     /translation="MNRALCALLLALALVLPAGAAEPEWDIEVGIDNSIYPSLIIATS
                     TMVEDEEEEPDPTMLGDPWGLIGVNIVAPAARTPVRVEMSSGKLIQPSVWEGVLEKKG
                     ETYLVCPVLKFDYDALLAVRQPFPEVVTAKVTVNGQLLPEKSKRVPVRSINDCILAFA
                     DDEEEVTDTSWLIAAYVNENHPVVDQILAEALDAGYVDSFAGYQKEASEVKKEIKAVY
                     RAIQDRGFKYSNITRGSGESATVGAQHIRLIGDQVRGSQANCVEGSVLFASILRKLEM
                     DPFLVLVPGHMFVGVFLDENQEDALCIETTMLGDSSFEDAVATAQQQFEEALPYISGE
                     EEPEEGDATDYTLIDIATARQLGVMPIREAAADSAPAAVPAAHAQRPAPSVADEESSG
                     DGEAVEEEAEAEPEGEDAEADYELETVKVKELGASIDLPGDWETEVEEDSAGDPLFTA
                     SNGDGDLVVNIGIMDAQDMEASALLKTYLEGLGMKREGAVEKMDLAGLPSAIVGASGD
                     MEGEAILATVMICRSGEKAYIVQALGAQEAFTDSQDMLVEIISSFRAR"
     gene            complement(7794..8207)
                     /locus_tag="PK634_04710"
     CDS             complement(7794..8207)
                     /locus_tag="PK634_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97070.1"
                     /translation="MRIALALVVGLFVCGMVMAQEVAAPVAAAEQAVTKAAAATETAA
                     EQVTAAAETAVETVASTVESAQVITLKGRVSVVKEADGSVRAIYINPEAGHGYKVDLK
                     NGEGMTLADKHGKVVEVTGADVNMLFQVKTITVVE"
     gene            complement(8422..9057)
                     /locus_tag="PK634_04715"
     CDS             complement(8422..9057)
                     /locus_tag="PK634_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97071.1"
                     /translation="MAIGGYLSSTPSTQYTIEFFGNSDCDPTGHGEGEVYLGNQFVIT
                     DAAGHAGFTNSLLAKPTPPPSFVTATARDIMRFETSEFSRYLMVDSDGDGMADGWEYS
                     NFGSVTGGDPAGHSDDDGVPNLDEFVAGTEPFKAFDYPRMSSISVSGATVFVSAPTSP
                     SRVYTLEFNQWVDVGSWGASLPARLGTGEPISWGWPVGVTTMYYRCTVALP"
     gene            complement(9094..10893)
                     /locus_tag="PK634_04720"
     CDS             complement(9094..10893)
                     /locus_tag="PK634_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97072.1"
                     /translation="MKAGCWIAVLCFSLGLPRLAAATTYTVTKTADSGDATLRWAIEA
                     ANTNPGPDTIAFSIAAAPYRIRPASALPGIFGGGGPVTLDGTTQPGYAGVPLVTLHGG
                     TAGGEGLYVMNSTSVVIKALVITAWATGVKMQDCVSNRVENCHIWTNTFDGIYMQRTS
                     WSVVGGSTNRGNLVASNGAAGAFILDNSRGNLVSGNRIGTDGLAALPNERGIYLADVD
                     GTVIGGAAASNRNVISASINDGILLSSWASNTTISGNYIGTDQTGTNAMGNRSGVTVS
                     GSRNSIGGASAGARNIISGNSSRGVDLSTADAHDNTVRGNWIGLGAGGQPCPNLYGVF
                     VSGGASTNTIGGSAAGERNVISGNTGDGVYLDGATTTNNTVSGNYIGTGTSGLTRAGN
                     AMDGVALRAPRNRIGGSAAGEGNLISGNGGHGISLANANSRGNIIRGNWIGVNATGNS
                     AINNDHDGIFIIDVADTLIGGAGGAMNVISANGENGIFLSGSGCANTVIAGNRIGLAA
                     LFFADLGNGGAGVLMDAAPSNSVGTGLPGEANVIANNDGDGIRVRDSAAIRNRFIENS
                     LYNNGGLGINLGTDGVTTNDPLDGDTGPNQYQN"
     gene            complement(10979..14734)
                     /locus_tag="PK634_04725"
     CDS             complement(10979..14734)
                     /locus_tag="PK634_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOW97073.1"
                     /translation="MNARLLASVVVLALVSALCSAQVPAVMNYQGRLIQGTNLYNGQV
                     GLSLQLWSAATGGLMLGEDSNTVTAVDGLYATVLGDDMPAGTLAQILNDNTNVYVRVL
                     VNGAALAPRERLASAAYAVIPGLKGVGNVQAGVHSAVGGGETNAVFGAASVIGGGRGN
                     LVDTNARESVIGGGERNWIATEASWTMIGGGLNNRIDTQSYCSVIGGGAGNCVTTLCA
                     YSVIGGGWSNKIHNYADYAAIPGGRENEAGGDFAFAAGRRAKANHQGSFVWADSAGAD
                     FRSTASNQFLIRASGGVAIGTNVSLGALTVAGVVNAESLVGDGSGLIMLDGQNIQAGS
                     ISNAALGVGSVRGTNLAAASIGPDKMTVKYWEINGNNNVTAGVHFVGTTTTNPLEFRV
                     SNARVMRFENAAVDSDPNVIGGHTYNLVAPGRVGATIAGGGGFLQHNEALDNYCAIGG
                     GAGNIAGDDTSKEYATVAGGEDNRAEGGWSTIPGGSDNRASGFYSFAAGRRAKAVDDG
                     AFVWADATDADFSSTANNQFIVRAAGGVGLNESDPRQQLSVGSCLDLYSGASNAPSQP
                     SIRGSSLGNLFLNATNDTYINYDSGHDLIVNLGGGRVGIGTSSPAHALGVAGNGGFSG
                     VVYADSFVGDGSGLTDLDGGNIMAMTVTTDQLAQAYWRLDGNANVAAGTHFIGTVIGV
                     PLDLYAGNTRVLRLGLNHNVLGGDEANTIGGGAVGGTIAGGGNGSFPNRVELNYGTVG
                     GGQGNAAEEVDATVGGGKSNTAAGPATFVGGGRNNTASGTNSVIAGGNNNSAAGAVSF
                     IGGGTNNSVAGAYATLGGGRGNRIGNEGDYGVIAGGISNYLDSMEATIGGGRGNLAAD
                     LATVGGGESNKVHGRGAVIAGGARNSIDALSPYAAVGGGRYHTVEYYATNAVIGGGEE
                     NTITTATRGTIGGGWRNRIDHGDYAFIGGGTSNMTAGAHSAIGGGIGNAITINAPQAA
                     IAGGYGNSVNANAGFIGGGSNNEVHVRSSSVVGGENNMASGEVAVVCGGLQNDVRGFG
                     AFIGAGQDNSATGDLAVVCGGMDNDANGRYAVVPGGRYNVATGNKSLAAGYRSKAGHE
                     GAFLWGDSTEADLVSTAADQFMVRARGGTIFYTDAAMSAGVELTAGAGSWTTLSDRNA
                     KEDFRAVDAREILDRLAELPIATWKYKAQDAAIRHIGPVAQDFRAAFGVGEKPTGITT
                     VDADGVALAAIQGLYEVVKEENARLRSENDELRQRLSAIEQKLGFEP"
     gene            complement(14803..17664)
                     /locus_tag="PK634_04730"
     CDS             complement(14803..17664)
                     /locus_tag="PK634_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97074.1"
                     /translation="MNTWFKKALLVAAAAVVSGPAEGATYIVTNSANSGNGTLRAAIS
                     NANLGAGTDLVVFNLPAGSRTIAVSTELPRITDRIRITGTNHLASGSEPLVTLRAASA
                     VVTNGLFLGAIGCTVTAMRVHGFEAGIMVTSPSCVIQRSWFMSNTDAGVCIKAGGTQI
                     IGNYFATNDTGIQLVQGTTNSVLANYVGIHPLTGVADGNWRGIFIASDKNYIGSPSAD
                     DRNIISGNRLRGIDISWISSSNSIRGNFIGTDAAGTGAVANSEGILINGNDNFIGGTK
                     GASGNLISGNRFNGITMDGLAQSVKRNRIEGNYIGTDVTGSNAIPNDTGIYMEEILCQ
                     SNTIGGALTAARNIISGNTNVGINLYYAPQNTIKGNYIGTDVSGRYALGNGSHGIHIE
                     HAWGTTVGGTNAGAGNVISGNGRHGIFIFGELSDDTVIERNHVGVTAAGGLAISNGWS
                     GIMVSGAPLVTIGGIGAGNVVSGNGRYGIYITSANAHDVTVQGNWIGTTASGQGALGN
                     RYEGVYVDDAPSNRIGGVSAAAGNVVSGNGAAGILITGALSVSNVVQSNFIGTDEDGE
                     DPLPNAREGLWLDTLSGCRVGGDLLVGGGNLISANGTDGIRLINCTDCVLLGNRIGAH
                     AEGAEFGGNTDDGIVITGASTNILIGGDVWTEINIISYNGRNGIVLGNSTNTTGIRID
                     YNWIFDNGGLGIDLGADGVTPNDGGPVYGDGDDGPNGLQNFPVITNVFVDGVDLRLQG
                     FLRSGVSQTYKINFYANTQFDPSGCGEGQISLGSTNVRTSLDTAHFNATVPAPDPVPD
                     YYAATATHIASGDTSEFSHFAMLDSDRDGMPDGWEARYFGSATGGDPAGHGDDDGMPN
                     LDEFYADTDPTDGESFLRIARASTVDYPTHRVTVVTYPSSAYRQYAVEHSDLPGLVTN
                     GWAAGLVGYEAGTGGIATNLDALPPGTTAAVFRVRGMLP"
     gene            17870..18352
                     /locus_tag="PK634_04735"
     CDS             17870..18352
                     /locus_tag="PK634_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97075.1"
                     /translation="MKILLWFLFRAVLAVFCFVYGFKLLFWGHPGAGLVGCGVLLAGA
                     WVIAFPIARILAAPFSAIFWPTGAEPPPPVYGIPESHVKKGLYAEAIREYHEIIRKWP
                     HELKPYVDLLDLAVRRMRDPDLAARIHGEALLKLKKPEERETVTRLYEAMKEARHEEG
                     "
     gene            18339..18596
                     /locus_tag="PK634_04740"
     CDS             18339..18596
                     /locus_tag="PK634_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOW97076.1"
                     /translation="MKKDDLQAIPGVGPSIAADLRSLGVTRVRDLEGRDPQRLYDALV
                     RRTGAPVDRCVLYVFRCAVYFAGHSRHDPEKLKWWNWKDPR"
     gene            18593..19102
                     /locus_tag="PK634_04745"
     CDS             18593..19102
                     /locus_tag="PK634_04745"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOW97077.1"
                     /translation="MKRILVAIIGSGATDKPALIEAARAAGEAIARHGAVTVAGECGG
                     VFGAAFAGARAAGGLTLALTGGDRVPEGHPVADVVLPTGLGPARHAVLAAACCGAVVI
                     EGGMPTLALAAEFLAARKPVVVVAESGGAAGLLAGSSALPGGTRVLSAFDGRAAVDLI
                     FQILGETNP"
     gene            19099..19374
                     /locus_tag="PK634_04750"
     CDS             19099..19374
                     /locus_tag="PK634_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97078.1"
                     /translation="MKSIQNVLAAVVLALWMLVSLSLATAFYMWIGVKAGPGALGEME
                     RALGGITLFVHPGFCLRILWAQVLFCFPVLLAMAVPAAWWLRRWYRT"
     gene            complement(19426..26484)
                     /locus_tag="PK634_04755"
     CDS             complement(19426..26484)
                     /locus_tag="PK634_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW97079.1"
                     /translation="MKVIRIAASFCFLVSMVFPAAAVKVRVTGADAVRQLTDAGGTLI
                     AEYESFSVMEADAAAVAALGDAAEALDDQIRLNAGAVDPSSAEVRSARRLATGFTGKR
                     LHLVQFAGPVKPEWHEELAATGARIVSYIPDNAYLVYSDSDGVTRLQANQARRAYIQW
                     EGAYADTDKIQPGARTTDAQGKARAIGTELFSIQMVADPEANAETLALVDSLKLAPIR
                     SRSENARLGFLNVTVALPADRLADLAARPDVVSIHPYFPPRPFCERQDQIVAGNLNVA
                     GSQPSGTGYLTWLYSKGFTQTQFDASGFIVDIADDGWDNGVAATPANPEFRVSGSAAQ
                     PSRMKYSQWGSTLASAGSWGTDGHGNINISIVGGYNDLTGSPYEDASGYNRGLGVCPF
                     ANLGNTKVFDDAGDWAPTDEQELQYISSNYNKGVRISSDSWGMPGDGVYDVYAQNYDT
                     VTRDAQPGVAGNQECLFVFAAGNDGPTSTTIGAPGTAKNIISVGAAENYNMFGTDGCA
                     VDDTGADNANDIIDFSSRGPCVDQRKKPDIVAPGTHVQGAASFYSGYTGNGVCDKYQP
                     AGQTNYAASSGTSHSTPAVAGGCALVRQHFINQGWTVPSPAMVKAYLMNAARYLDGVD
                     TGDTLPSNNQGMGMMNLGTAFDGAARILRDQLTNDLFTASGQSRRFYGQVSNTGLPVR
                     VTVGWTDAPGSTSGNAYNNNLDLTVVAGGVTYKGNVFSGAYSTNGGTADARNNSESVF
                     LRAGTTGLVQVTVSATSINSDGVPSYGGALDQDFALVVANATAFVPTNVPPSLDPVGH
                     KTVTTNNLLQFTVSASDTIDNDSIRLWAEGLPPWATFAGATNASTVSSQFSGTPLVTG
                     TYAVTFYAADKDGTNSETITVVANDIYCIPTNIIDEGFDGGTTAPAGWTFTSIGGTYA
                     TAGNYGRASPSLKFDLDGDRVETPALNMPSNVTFWIKGQGTDASSFLLVEGYNGSSWS
                     TVGTIVPILTTATTQSLAIAEGISQLRFTYDKSAGNLAFDDVIIAGCGGGSPVNREPT
                     ISAAGGTNQTVTTGSELTFVVTVNDADAESVGVYTSAAPAGANFPTVSGTAPIQSTFT
                     WTPAETGTFTAVFVAFDAQVSVTQGVTIVVNEPAPELLPPVIQAATGVQGTQFNANWL
                     ASANAAGYRLDVSTNSSFTGSGGATTNLAEDFALFTRTNSSADIGGSLDTYTHTAGWT
                     GTKVYENIGTAKIGSSSAKGYITTPTVNLSGNGGAATVTFDLGKYGTDVNAVQVMHAA
                     DGSTFVQVGSDLIPPASMALQTVELTGGTAASKIRITAKGISNNRFYLDNFAIAQGAA
                     GGSYVLGYQARDVGDVTTEPVTGLTENVMYYYRVMAYHATSNSAYSGVTNVLTTAAPD
                     QPPTFTAPASTNQAGTITNLLSFVVSASTAPDADDVTLWAEGVPAWATFATVTNAGGV
                     TNTFSGTPPAGSTNVVTFYAGDKDGTNSRAVQIVVTGGAEVLLPPVILAASGVQANQF
                     DANWQASAGATGYRLDVATNNQFRHTARLPKVSSLSPGDLMMVTADADATEGFDVVPL
                     VDLDAGTVIYFTDNGWSNGWRASEGTVTYTAPGAITAGTVLSYRSVDENGFVKSGSFD
                     LSASGDTILAYQGSAGSPQFLYGIGWAIASPWVTAGTVLTANTSEMPSGLSTGAYTIV
                     ACGTSDNYQYSSANGTSGTKAELLQKTGTAANWAGNDTTAYAKFTPDFTVSSGGGVND
                     YVPGYEDRDVVNVTTYGVTGLAVDVMYYYRVRAYNLTSNSVNSAVTNVLTTAAGDIPP
                     TFTAPASTNQAGTITNLLSFVVSASTAPDADDVTLWAEGVPAWATFATVTNAGGVTNT
                     FSGTPPAGSTNVVTFYAGDKDGTNSLAVQVIVTGGAEVLLPPIVQAASNVDTNQFDAN
                     WLASAGATGYRLDVATNNQFRHTARLPKVSSLSPGDLMIVTVDADDPEGFDVVPLVDL
                     DAGTVIYFTDNGWSNGWRASEGIVTYTAPGAVTAGTVLSYRSVDENGFVKTGASFALA
                     AAGDTILAYQGSAESPQFLYGAGWAIASPWVADGTALTANNSCIPSSLSTGAFTIVSC
                     GSFDNQQYNSASGTTGTKSALLQLVANAANWSGSDATAYAKFTPDFTVSSGGGVNDYV
                     PGYENRDVANVTTYGVTGLAVDVMYYYRVRAYNATTTTANSATTNVLTAAPSGTPPVL
                     NAIGNQSVGIGSNLHFAVTATATEADTVTLTASNLPAGAFFYPTNELASFVWDAASPT
                     GTYTVTFNAADNDGSDEETITITVNGLPVEEPDITGFAVPAGATAAATLTSVNGQSYT
                     LEYTTDLGAKPPVWTPADTKIGDGGAITLSDPTPADMLRIYRIVTP"
     gene            26742..27710
                     /locus_tag="PK634_04760"
     CDS             26742..27710
                     /locus_tag="PK634_04760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOW97080.1"
                     /translation="MDTADFSHLTPEAVINAVEATLGVPCTNICRPLNSYINRVYEVG
                     LDDGGYVIAKFYRPGRWDRVALQDELDFLAELAEEEVPVVPPLPGRDGALLHDLAHTA
                     FAIFPKRGGRPLDEPDANTWTQLGRLLARMHAVGARRLPKNRVTLHPEHSTRQHLDFI
                     LRSGTIPSGPARAYERLVTELIELVAPDFEGVETHRIHGDCHRQNILHRPGEGFHLLD
                     FDDMAVGPAVQDLWMLLPDRVRAARVELESLLEGYETFLPFPRETLGLVEPLRAMRFI
                     HYTAWCARQKADGGFARLAPDWGSQAFWRQETADLERQRQEILDAR"
     gene            28394..28783
                     /gene="crcB"
                     /locus_tag="PK634_04765"
     CDS             28394..28783
                     /gene="crcB"
                     /locus_tag="PK634_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOW97081.1"
                     /translation="MRLLFSMLLVGAGGFVGSVLRYALALAAQRYSISFPHGTLWANL
                     LGCLAIGAISALAAATEVLSPAVRLLLATGICGGFTTMSSFIYELAQLLRDNELWLAA
                     GYFGLTLGGCLAMFWIGALAVKLLLKA"
     gene            28791..29144
                     /locus_tag="PK634_04770"
     CDS             28791..29144
                     /locus_tag="PK634_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOW97082.1"
                     /translation="MELKGEAKLLRIFLGESDKVRHTPLYEAIVKEARSAGLAGATAW
                     RGILGYGPTSRIRSAKILDLSSDLPVVIEINDDEEKINRFLPKLHELFETAQSGGLIT
                     IEKVQVIKYLHGNPA"
     gene            29182..30558
                     /gene="rlmD"
                     /locus_tag="PK634_04775"
     CDS             29182..30558
                     /gene="rlmD"
                     /locus_tag="PK634_04775"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOW97083.1"
                     /translation="MTREIELTAEKMVAEGRALARPDGFVVFVGGALPGERVRARITQ
                     RKKSFAFAQALEILDPSPDRQAPPCPVFGQCGGCAFQHMAYPAQLRAKHQILIEALYG
                     LPGVPGIVAEPLGMEEPVHFRNKMGFAFGMAEGRAVVGLHRRGDWRSVVPAGTCLLQS
                     PESREILARVLAFVQDQRLPVYDEEKQQGLVRHLVVREGKHTGERMVHLHAAERHPAF
                     EQLPAVLGDFASTVLASFHTQVPESAPPGSTTVLSGPGLIHERLNGFTFEIGPATFFQ
                     TNTRQGERLFGLVRDWAADCRPERAVDLYAGTGPIAIHLAAAASQVLGIESHAPSVDA
                     ARRNVELNILSNVRMVCAEAERVGETLREERADLVVVDPPRPGLHPKAKAALLSAAPP
                     NLIYVSCNPATLARDLAKLVESGYALERVQPLDMFPHTFHIEAVALLSRKMNAQHPTA
                     TAHRPGAW"
     gene            complement(30657..31046)
                     /locus_tag="PK634_04780"
     CDS             complement(30657..31046)
                     /locus_tag="PK634_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97084.1"
                     /translation="MMKKMMLLMSVAGFVAASLSARAGGACGAAAAGSKSAGGCGAKV
                     EKAEAAAAPSAQPSPAVQPAGEVKAQTACPVMGGAVNKSIYVDHNGKRVYLCCNACVS
                     TFKADPDKYVKQLTDAGVQLEDAPAAQ"
     gene            31273..>32904
                     /locus_tag="PK634_04785"
     CDS             31273..>32904
                     /locus_tag="PK634_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOW97085.1"
                     /translation="MAKKSEKVLSAPAPEEVQPAAPALAEPAAAGAPEPAKRPAGGAK
                     YDAKSITVLGGMDAVRKRPAMYIGDTGARGLHHCVFEVVDNSVDEALAGYCQRIVVTM
                     NSDGSVSVLDDGRGIPVDMHATEKRPALEVVMTVLHAGGKFDHDSYKVSGGLHGVGVS
                     CVNALSEWMEVEVRRDGGVYHQRYERGKAVSNVEAIGKTKSSGTKVTFYPDHKIFPSL
                     EFSWDILANRLRELAFLNKGIEITLQQEDPAREETFKYKGGIVEFVEHLNKNKNPLHP
                     KVVFFEKEKDRIQVEIALQYSDAFNENIFTYTNNIHTIEGGTHLSGFRSALTRVINQY
                     AKANKLLKGDDETMSGDDVREGLTCVISVKVPDPQFEGQTKTKLGNSEVEGIVASIVN
                     EELGTYFEEHPSVARRIIEKGVLAARAREAARKARDLTRRKGALDSGGLPGKLADCSE
                     KDPELCELFIVEGDSAGGSAKQGRDRRFQAILPLRGKVLNVEKARLDKMLNNNEIRTL
                     ATAIGAGIGKDEFDVAKARYHKVIIMTDADVDGLHI"
BASE COUNT         5129 a        10838 c        11187 g         5750 t
ORIGIN      
        1 tggaaagccg gactctcccg actgttgcgg acggcaattg ccatcagtct ggtcttcgcg
       61 acaaccggca tgaatctcca gacaagtggg aagtgtccgg gtggtggcgg cgacatcacc
      121 gaggtgttca ccaccttcgc cgtcatgacc tacttgccgc tagcgggagt tcttcttgtt
      181 gatttgatcg caaggctcat ctttaaatgg gaagacaaga aggacagcga accagcaact
      241 ccaccctatt cggagcccgc tgcgcggtct cctcaagggt gagttgcgcc gttcgcacaa
      301 aggaaacatg gaggaggggg gggcgcgcat gagaactcta gccttggccg tcgtcatggc
      361 attgatcggc tcgattgccg tgtttgctga ggacatcgcc atcaaggcgt tcgatgtcga
      421 ggggaggata tccttcgatg aggttgcgga tgcgtggatg tatcaagtga actggaccac
      481 caatcttgga gagaccactt ggcgcacgtc atcaccaccc ggtgtggcgc gctttgctgc
      541 ccagggggac gaaacgcggg ttgtaaccgt gggccttcag aacgcctcat gcttcttccg
      601 aatcgaggcg acaaactacc cccccgccac ccgaaggaat ggtcctgatt ccagccggaa
      661 ccttcgtgat gggctacgcg accaatcttc tccaaggacg aagcgggaga ggggcaacta
      721 ccgcaacacg gcgtcggcat aagccctttt tgcatcgaga agtacgaggt aaccaaggcg
      781 ctgtgggatg aagtcagaac gtgggctctc acacaaggct atgcatttga gaattcaggc
      841 tcgggcaagg gcacgaatca ccccgttcac acagtgaact ggtacgatgc tgtgaagtgg
      901 tgcaacgcgc gcagcgaact ggccagtttg acaccgtgct acacgacaaa tggcatgacc
      961 tacaagaccg gcaacaacag caacgtggtc tgcaactggt cggccaatgg atatcgtctg
     1021 cccaccgagg ctgagtggga aagagcggcg cgcgacggag ccgcgtacac gcgattcccc
     1081 tggactgact acacgaacag gatttcgcat gccaaggcga actactgggg atatacgggc
     1141 gtgggttacg atctgagcag tggataccat ccaacgtatg ccaccggggg tactccgtac
     1201 acaagtcccg tgggatcctt tgacccgaat ggctatggga tatacgacct gtcagggaat
     1261 gtgtgcgagt ggtgctggga ctggctcgat ctgaactact acagcaattc acccccctcg
     1321 gatccacgcg gcccagattg cggcgcatct cgagtcatga ggggcggggc atggggctac
     1381 agctcgggat acgccggttg tgccggccgg tactacaacg gccctgtgaa tgagaattac
     1441 gagtgggggt tccgatgcgc aaggggtctc aatccatgag atcgcttcca tatcccgcat
     1501 gcacgccgga agggccgcga acttcggacg gtaccagccg gcatatgcgg agacggagca
     1561 agtatactct gcgaaccgtc gggaggagcg ggcgttttgc ccgaggcggc caaaacgtcc
     1621 gcttgcccgt gtgacgcggg caaaacgtat ccggcggctc ctcggcattt acaccctccc
     1681 tgtgcaactc tatattctcc aagggccggt tcgccgtcac cgcggcaagg ggccttgcct
     1741 gcaacgcagc gtccgtgggt gtgctgagcc cggcccggcg gctgatgagc gccggggcgc
     1801 ggccggatcg taacgccgtt cacccgggaa agagagggcg acatcatgat gctgatggct
     1861 ccacgccgag ttattgcgtt ctccctggcg acatggctgc tgccggccga cgtgagccgg
     1921 gacgggacgg agcaggaggc gcggaaatga tcaagggcgg atgttattgc ggggccgtcc
     1981 ggtacgaggt tcgcggccgg ctgctcctgt tcgcgaattg tcattgcccc gattgccgga
     2041 aattcaccgg atcggcgttt gcctcggtcc tggtcacgga aacccaggga ttcgaggtcg
     2101 cgtccggcga agagcagctc gttccgttcc agtcctcccc gggcaagcgg cgcttttttt
     2161 gccggcattg cgggtgccac ctcttttccc gcgcggagca gcgtccgggc ctggtcttcg
     2221 tgcgcgcggg ctctctcgat gacgatccgc cgatgcggcc gcagtgccac atctggacca
     2281 gcgcgaaagc gccctggcac gagatcagcg actcgctccc gcagcaccgg gaggggctgc
     2341 ccaggaactg accgatccgg cgggcgggag cctctctggc gtaagcggtg ggagtcgtct
     2401 gcaggacagc cgtccctctc cttgccatgc cctcgcggag aacgcgggcg ccggtcgtgg
     2461 gccaggatgg cgtggcggcc cacctttttt cccagtcgcc agccggcgcg gataacggct
     2521 tgaacggccg cccgttccga aatgttggtc agcaggccca aggggcgctc cccggtcatt
     2581 catcctacat gccctgcccg ggcggggaag acatgttcag agatcgtctg aacgcgcgcc
     2641 ggcatgtccg gtatactacg ctggtgttgc cggcgaacgc cggccggggt gcggagggcg
     2701 gggtgttatc atgaaactcg aagcggcgtc ggggcgggcg ccccccggcc ttctggaatt
     2761 gctccgggac ctcggggacg gcgaaaacgg attcatgggc acccccgtgc cctcgggaga
     2821 gatgggcctc gaagagtacc tgcagcgatg cgtggagggc ccggatccgg cccggctgcg
     2881 acccgggctg gcgccccaga cggttttctg gatgatcgac gacgacggcg tggccgtggg
     2941 catggtgcgg atgcgtcaca gcctgaacga ccggttgcga gcgcacggcg gacacatcgg
     3001 gttctacgtg cggcgcgacc ggcgcgggcg cgggcatgcc cgggcggcct tgcgcatggc
     3061 gctggccgaa ttggcgaaac ggggcgaacg gaaggcgttg ctgacggtgg cgacggacaa
     3121 tgcgccctcg ctccgggtcg tcgaggcctg cggtgggcgc tgcgacgggg agagcgtgga
     3181 cccggaaagc gggcggcggc tgcgacgcta ctggattgac ctcccgccgc cggccggaag
     3241 accagaataa aaggagcgat cccatgagaa aggccctatt cccggtcatg ctgctggcgg
     3301 cgctcggcta cggggccgtc ggagagacgc cggcgccggt cgccccggac caggtggtcc
     3361 tgctggacgt ccagggcctc tggggcggag tcgatctgtg gattgcgaag gacggccggg
     3421 ccgtttgccg cctcgtcgga ccgcccaagg cgggcggccg agggctgcag gaatcccggt
     3481 atgccttcgc cctttccgcg gaacagcagg cggaactggc cgcgctcgtg gatgggcacg
     3541 gcttcttcac gctccaaacc gcggatcgcc agggcgtgcc cgacgaatcc cggccagcga
     3601 tctttatccg gacgggcgcg cgtcgccgcg cggtggccaa gtggggcaac gacgcgcatc
     3661 cggattttga tcccctctac cgcttcctgc tggccgccgc ccgggccggc cggacgggaa
     3721 cgctgttggc ggagggcgcg tacgagcccg actggcaacc ggacggcttc gagaccggcc
     3781 gcgcgatccg ggacatgacg cggacgccgg cggacccgcc gccggccgag tagcccggac
     3841 gcctgggatt gaggcggcgg cgagcaggcc gtatagtctt gtcgccgcga ccggaaggaa
     3901 aggaagtcat catgatcaag ggcggctgtc attgcggggc cgtgcgttac gagagcgacg
     3961 ggcgcgtgct gcgtttcgtg aactgccact gcccggactg ccgcaaatct tccggctccg
     4021 cgttcagctc ggtgctggcg gtggaggccg gcgggttccg cgtggtcgcg ggggaggatc
     4081 gcgtggttcc ctatgaatcc tcgcccggga agttccgatg cttctgccgg acgtgcggct
     4141 cgcatgtctt cgcgcgcgcg gcgcagcggc cgggcatgat cctgattcgg gcggggacga
     4201 tcgacggcga ccccggggtg cggcccgaag tccatatctg ggtcggcgaa aaagccccat
     4261 ggcacgagat cggcgatagc ctgccccggc acacggaggg cctgccgccg aaatgagcag
     4321 gcttcccgat cccggcgggc ccgtctactg cgaggaattc ccggttcccc cgcaggccgt
     4381 cgacgtcaac ggccacgtga acaacgtcgt gtatgtccag tggatgcagg acatggccgt
     4441 gcatcattcc gacgcggtcg ggggcacgcg ggccatgagc gaggcgggcg gccggtgggt
     4501 cgtgcgcgcg caccgggtcg agtatctggc cccggccttc gagggcgacc ggattcgcgt
     4561 cgccacctgg gtggccgatt tccggcgcgt gcgctcgctc cggcgctacc gtttcgagcg
     4621 ggcgtccgac gggctgctcc tggcgcgcgg ggagacggac tgggttttcg tggacgcggc
     4681 caccggccgg ccacggactg ttccggacga tctgcgccgc cggttccccc tccatgccgc
     4741 ggcgggcccg gaggcgggcg agtgaagagg ccgtccaggc cgatggatgc cgcggcgcgg
     4801 gagcgggtct tggcggaact gcatcgggac gtcgccctcc gccagcaggg ctaccgcgag
     4861 cgcgccctga agctgttccc ccacgtctgc gcccgctgcg gacgggcctt cgaggggcgc
     4921 cgcctgcgcg aactgaccgt tcatcaccgc gaccacaacc acgacaacaa cccgcccgac
     4981 ggaagcaact gggaattgtt gtgcctctac tgccacgacc acgagcatga gggcaacctc
     5041 atggccggcc tcggggccca ggccggcggc gaaggggcgg ccccgggcac gttcagccct
     5101 ttcgagaacc tggattcgct gttgaagaac ggggccccgc ccgaagcggg ccgtccggga
     5161 gaaaagccat gaaaaaaggc ctcgctgttc tgctgatgct tgccgccgcc gcccgcgcgg
     5221 aggagcgatt catgttgtcc gatccggaag tcggcgcgga gtacgggccg tttgtctacg
     5281 aggatggcgc catcctcgag gtctacggcc ggcccctcac gctccggcgg ctcgatacga
     5341 aagccgacgc gctcaagcag aagatggagc agatcacact cccctccatc gagttccgga
     5401 acgccgccct gccggacgtc gtgcagttcc tcgtggacgc gagcgttgcc ggcgatcccg
     5461 aggggacggg cgtcaatatc gtcctgccgc cggaaccggc cgccggcgag gcggcgccgc
     5521 gcaccgtcac gttgagcctg cggcgggtgt cgctgtacga cgccctggcg ctcgtcgccg
     5581 aggtctcggg gctgtccgtt cggctggacg agcgcggagt agtcctgctc gaggccggcg
     5641 gcagggcggg aggcgcgagc ccgtgatcca acggtgcgcg acgtccggcg cctgctggcc
     5701 gacccgggca tcgtgcgcaa ccgcctgaag atcgaggcgg ccatccggaa agcgcgcggt
     5761 gttctcgcca tccgggagga gttcggctcg ttcgacgcct acctgtcgag gtttgtgggc
     5821 ggccggttca gagccaatgg gccggattga atgaagtgcc ggcccgcagc gtcatatcgg
     5881 acgccatgag ccgggatctg aaaaagcgcg gtttccattt cgtgggcgcc acgatatgtt
     5941 atgcgttcat gcaggcggtc ggcctggtca acgaccacgt cgccggctgt tttcgacgcg
     6001 cggaaattcg gaaaatggca cgggccgaaa tatcgaggag aaaaccatga atcgtgcgct
     6061 gtgcgccctg cttcttgccc tggccctggt ccttcccgcc ggggcggcgg agccggagtg
     6121 ggatatcgag gtcggcatcg acaacagcat ctacccctcg ctgatcatcg ccacgtccac
     6181 gatggtcgag gacgaggaag aggaacccga cccaacgatg ctgggcgatc cctggggcct
     6241 gatcggggtg aacatcgtcg cgccggcggc ccggaccccg gtccgcgtcg agatgtccag
     6301 cggcaaactg atccagccca gcgtctggga gggcgtcctg gagaagaagg gcgagacgta
     6361 tctcgtctgc cccgtcctga aattcgatta cgacgcgctg ctggccgtcc gccagccctt
     6421 cccggaggtc gtgaccgcga aggtcacggt caacgggcaa ctcctgcctg agaaaagcaa
     6481 gcgcgtgccc gtccgctcca tcaacgattg catcctggcc tttgcggacg acgaggagga
     6541 agtcacggac acctcgtggc tgatcgcggc ctacgtgaat gaaaaccacc cggtcgtgga
     6601 ccagatcctg gccgaggccc tggacgccgg ctacgtggac tccttcgcgg gttaccagaa
     6661 agaggcgtcg gaagtgaaga aggagatcaa ggccgtctac cgcgccatcc aggaccgcgg
     6721 cttcaagtac agcaacatca cccggggctc gggcgaatcc gcgaccgtcg gcgcgcagca
     6781 catccggctc atcggcgacc aggtccgggg cagccaggcc aactgcgtcg agggcagcgt
     6841 gttgttcgcg tccatcctgc gcaagctgga gatggacccg ttcctcgtcc tcgtgccggg
     6901 ccacatgttt gtcggcgtct tcctcgacga gaaccaggag gacgcccttt gcatcgaaac
     6961 caccatgctc ggcgattcct cgttcgagga cgccgtggcg accgcccagc agcagttcga
     7021 ggaggccctg ccgtacatct cgggcgaaga ggagcccgag gaaggcgacg ccacggacta
     7081 caccctcatc gacatcgcca cggcgcgcca gctcggcgtg atgccgatcc gcgaggcggc
     7141 ggcggattcc gcgccggccg cggtcccggc cgcgcatgcc cagcgccccg cgccgtccgt
     7201 cgcggacgag gaatcttcgg gcgatgggga ggcggtcgag gaggaggcgg aggcggaacc
     7261 ggaaggagag gacgccgagg ccgactacga gctggagacg gtcaaggtaa aggagctggg
     7321 cgcgagcatc gatctccccg gcgactggga gacggaagtg gaggaggact cggcgggcga
     7381 tcccctgttc acggcgtcca acggggacgg ggaccttgtt gtgaacatcg ggatcatgga
     7441 tgcccaggac atggaggctt cggccctgct gaagacctac ctggagggcc tgggcatgaa
     7501 gcgcgaaggc gcggtcgaga agatggacct tgcgggcctg ccctccgcga tcgtcggggc
     7561 gtccggggac atggaggggg aagcgatcct cgcgaccgtc atgatctgcc ggagcggcga
     7621 gaaggcctat attgtgcagg cgctgggcgc gcaggaggcc ttcaccgaca gccaggacat
     7681 gctggtcgag atcatcagca gtttccgggc ccgctgaacc ggtccctcat ggaagaaaaa
     7741 accgcgccgg gattcccggc gcggtttttc acatccaaca gaccgcggaa gagttactcg
     7801 acgaccgtga tggtcttgac ttggaagagc atgttcacgt ccgcgcccgt gacctccacg
     7861 accttcccgt gcttgtcggc cagggtcatg ccctcgccgt tcttcaggtc caccttgtag
     7921 ccatggccgg cttccgggtt gatgtagatg gcccggacgg aaccatcggc ttccttcacc
     7981 acgctgaccc ggcccttgag ggttatgacc tgggcgcttt cgaccgtcga ggcgaccgtc
     8041 tcgaccgcgg tctccgccgc cgccgtcacc tgttccgccg cggtctccgt cgccgccgcg
     8101 gcttttgtca cagcctgttc cgccgccgcc accggagccg cgacttcctg ggccatgacc
     8161 atcccgcaca cgaacaaacc gacaaccaac gccaatgcga tgcgcatttt ctacgctcct
     8221 ttctagggta tgacgtatac tcttcggggt ggggcgtcac aagctaatac gggccatttc
     8281 aggcgcgaaa ggaacggacg caaaagcctg gattctcgga tcggccgcct tgcagctcgg
     8341 gcaggcgctt aaagcgtttg actgctcgtc ggaccgaagc gtgccatgat tggcgccacc
     8401 atccgtcagg ccgccccgcc gtcagggcag cgccaccgtg cagcggtaat acatggtcgt
     8461 aacccccacc ggccagcccc acgagatggg ctcccccgtg cccagtcggg cgggcaggga
     8521 cgcgccccag ctgccgacgt cgacccactg gttgaattcc agtgtgtaca cgcgagaggg
     8581 cgacgtgggg gctgaaacaa agaccgtggc gccgctgacc gatatgctcg acatgcgcgg
     8641 gtaatcaaaa gccttgaacg gctcggtacc cgcaacgaac tcgtcgagat tcggcacgcc
     8701 gtcgtcgtcg ctgtggccgg cgggatcgcc gcccgtgacc gatccgaagt tggaatactc
     8761 ccagccgtcc gccatgccgt cgccgtcgga gtccaccatg agatagcgcg agaactccga
     8821 ggtctcaaac ctcatgatgt cgcgcgcggt ggcggtgacg aagctgggcg gcggggtggg
     8881 cttggcgagc aggctgttgg tgaacccggc gtggcccgcg gcatcggtga tcacgaactg
     8941 gttgcccagg tacacctcgc cctcgccgtg ccccgtgggg tcgcaatcgc tgttgccgaa
     9001 gaactcgatc gtgtattgcg tgctcggcgt gctgctcaaa tagccgccga tcgccatggt
     9061 ggcgccggca ttggaggcca gggtgaggac cggctagttc tggtactggt tgggccccgt
     9121 gtcgccgtcc aggggatcat ttgtcgtcac gccgtcggtg cccaggttga tcccgagccc
     9181 gccgttgttg tagaggctgt tctcgatgaa acggttgcga atcgcggcgc tgtcccgcac
     9241 cctgatgccg tcgccgtcgt tgttggcgat gacattggcc tcgccgggca gtcccgtgcc
     9301 cacgctgttg ctcggcgcgg cgtccatgag cacgccggcc ccgccgttgc cgaggtccgc
     9361 gaaaaacagg gcggccagcc cgatccggtt cccggcgatg accgtattgg cgcagcccga
     9421 gcccgacaga aagatgccat tttccccgtt ggccgaaatg acgttcatgg ccccgcccgc
     9481 gccgccgatc agggtgtcgg ccacatcaat aatgaaaatg ccgtcgtggt cgttgttgat
     9541 ggccgagttc cccgtcgcgt tcacgccgat ccaattgccc cggatgatat ttccgcggct
     9601 gttggcgttc gccagggaga tgccgtgccc gccgttgccg gagatcaggt ttccctctcc
     9661 cgccgcgctc ccaccgatcc ggttgcgagg cgcccgcagc gcgacgccgt ccatggcatt
     9721 gcccgcgcgg gtcagtccgg acgtgccggt gccgatgtaa ttgccgctga ccgtgttgtt
     9781 ggtggtcgtg gccccgtcca ggtacacgcc gtcgccggta ttgcccgaga ttacgttgcg
     9841 ctcgccggcg gccgatcccc cgatggtatt cgtggaggcg cctcccgaga cgaacacgcc
     9901 atacaggttg ggacagggct gccccccggc gcccaacccg atccagttgc cccggaccgt
     9961 gttgtcatgg gcgtcggccg tggagaggtc caccccgcgc gagctgttgc cggagatgat
    10021 gttgcgcgcg ccggccgagg cccccccgat gctgttccgc gagcccgata ccgtcacgcc
    10081 cgagcggttt cccatggcgt tggtgccggt ctggtccgtg ccgatgtagt tgccggatat
    10141 cgtcgtgttg ctggcccagg acgacagcag gatcccgtca ttgatgctgg cgctgatgac
    10201 gttgcggttt gatgccgccg cgcccccgat cacggtgccg tccacatccg caaggtaaat
    10261 gcccctttcg ttcggcagtg ccgcgaggcc gtcggttccg atccggttgc cgctgacgag
    10321 gttgcccctg gagttatcga gaatgaaggc gccggccgcc ccgttggagg cgacgaggtt
    10381 gccccggttc gtggagccgc cgaccacgga ccagcttgtt cgctgcatgt agatgccgtc
    10441 gaaggtgttg gtccagatgt ggcaattttc cacccggttg gacacgcagt cctgcatctt
    10501 cacgccggtg gcccaggcgg tgatgaccag ggccttgatg acgaccgagg tggaattcat
    10561 cacgtacagc ccctcgccgc ccgcggtacc cccgtgcagc gtgaccaggg ggacgccggc
    10621 gtagccgggt tgcgtggtcc cgtccagcgt gacaggccct cccccgccga agatgcccgg
    10681 cagggcgctg gccggcctga tccggtaggg cgccgccgcg atggaaaagg cgatcgtgtc
    10741 ggggccgggg ttggtgttcg cggcctcgat cgcccagcgc agcgtggcgt cgccgctgtc
    10801 ggcggtcttg gtgacggtgt acgtggtggc ggcggcaagg cgcggcaggc caaggctgaa
    10861 gcacaaaaca gcaatccagc aaccagcttt cataatgccc tccttggagt aagtcgctcc
    10921 cgggcgaagg cttgtctcgc ctcggacaac attcctccat ccccttgccg cggaacagtt
    10981 acggttcgaa tcccagcttc tgttcgatgg ccgaaaggcg ctggcgcagt tcgtcgttct
    11041 cgctgcgcag ccgggcgttc tcctccttca ccacctcgta caggccctgg atggcggcga
    11101 gcgccacgcc gtccgcgtcc accgtcgtga tgcccgtcgg tttctcgccc acgccgaatg
    11161 ccgcgcggaa atcctgcgcg accggcccga tgtggcggat cgccgcgtcc tgcgccttgt
    11221 atttccaggt ggcgatgggc aactccgcca gccggtccag gatttcccgt gcgtccaccg
    11281 cgcggaaatc ctccttggcg ttgcggtcgc tcaaggtggt ccagctcccg gcgccggccg
    11341 tgagttccac cccggcgctc atggcggcgt cggtgtagaa gatcgtgccg cccctggcgc
    11401 ggaccatgaa ctggtccgcc gccgtgctca cgaggtcggc ttcggtggaa tccccccaga
    11461 ggaacgcgcc ttcgtgaccg gccttcgacc ggtagccggc cgccaggctt ttgttgccgg
    11521 tcgcgacatt gtaacggccg cctggcacga cggcgtacct gccgttcgcg tcgttgtcca
    11581 tgccgccaca gaccacggcc aggtcgccgg tcgcgctgtt gtcctgcccg gcgccgatga
    11641 aggccccgaa gccgcggaca tcgttctgga ggccgccgca gacgacggcc acctcgccgc
    11701 tggccatgtt gttttcgccg ccgacgacgc tggaactgcg gacgtggacc tcgttgttcg
    11761 agccgccgcc gatgaagccg gcgttggcgt ttaccgagtt tccatagccg cccgcgatgg
    11821 ccgcctgcgg ggcgttgatg gtgatggcgt tcccgatgcc gccgccgatc gccgagtggg
    11881 cgccggcggt catgttgctg gttcccccgc cgatgaaggc gtaatccccg tggtcgatcc
    11941 ggttacgcca gccgccgccg atcgtccccc gggttgcggt ggtgatcgtg ttctcctcgc
    12001 cgcccccgat caccgcgttg gtcgcgtagt attccaccgt gtggtaccgg ccgccgccca
    12061 ccgcggcgta gggggacagg gcatcgatcg agttgcgcgc gcctcccgcg atgacggccc
    12121 cgcggccgtg caccttgttg gattcgccac cgcccaccgt agcgaggtcc gcggcgaggt
    12181 tgccgcgtcc gccgccgatc gtggcctcca tgctgtcgag gtaattgctg atcccgccgg
    12241 cgatcacccc gtagtccccc tcgttgccga tgcggttgcc gcgcccgccg ccgagggtgg
    12301 cgtacgcgcc cgcgaccgag ttgttcgtgc cgccgccgat gaacgaaacc gcgcccgccg
    12361 ccgagttgtt gttcccgccg gcgatgacgc tgttggtgcc cgaggccgtg ttgttccggc
    12421 cgcccccgac gaaagtggcc ggcccggcgg ccgtgttgga ttttccgccc ccgacggtgg
    12481 cgtctacctc ctccgccgcg ttgccttgcc ctcctccgac cgtgccgtaa ttcagctcga
    12541 cgcggttggg gaacgacccg ttgccgccgc cggcgatggt gccgccgacc gccccgccgc
    12601 cgatggtatt cgcctcgtcg ccgcctaata cattgtggtt caggcccaat cgaagaacac
    12661 gggtgttgcc ggcgtacagg tcgaggggca cgccgatgac cgtcccgatg aaatgcgtgc
    12721 cggccgccac gttcgcgttg ccgtccagcc gccagtaggc ctgggcgagc tggtcggtgg
    12781 tgacggtcat cgccatgatg ttcccgccgt ccaggtccgt cagcccggac ccgtcgccga
    12841 cgaaggagtc cgcgtacacg acgcctgaga agcccccgtt gccggccacg cccagcgcgt
    12901 gcgcggggct gctggtcccg atgcccaccc ggccgccgcc caggttgacg atcaggtcat
    12961 ggccgctgtc gtagttgatg taggtgtcgt tcgtggcgtt gaggaatagg ttgcccagcg
    13021 aggagccccg gatcgagggc tgcgacggcg cattgctggc cccggagtag aggtcgaggc
    13081 agctcccgac gctcaactgc tggcgcgggt cactctcgtt caatcccacc ccgcccgcgg
    13141 cgcggacgat gaactggttg ttggcggtcg agctgaagtc cgcgtccgtg gcgtccgccc
    13201 agacgaacgc gccgtcgtcg accgccttgg cccggcggcc ggcggcgaac gagtagaaac
    13261 cggaagcgcg gttgtcgctc ccgcccggga tggtggacca gccgccctcg gcgcggttgt
    13321 cctccccgcc cgcgacggtg gcgtattcct tgctcgtgtc gtccccggcg atgttgccgg
    13381 cgccgccgcc gatggcgcag tagttgtcca gcgcctcgtt gtgttgcagg aacccgccgc
    13441 cgcccgcgat cgtcgccccg acccggcccg gcgccaccag gttgtacgta tggccgccga
    13501 tgacattcgg atcgctgtcc acggccgcgt tctcgaagcg catcacccgc gcgttggaca
    13561 cccggaactc gaggggattg gtcgtggttg tgccgacgaa atgcacgccg gcggtgacgt
    13621 tgttgttgcc gttgatttcc cagtacttca ccgtcatctt gtcagggccg atgctggcgg
    13681 ccgcgaggtt ggtgccccgc acgctgccca cgcccagggc cgcgttggag atcgagccgg
    13741 cctggatgtt ctggccgtcg agcatgatga ggcccgagcc gtcgccgacc agcgactcgg
    13801 cgttgacgac gcccgccacc gtcagggcgc cgagggaaac gttcgtgccg atcgccacgc
    13861 cgccggaggc ccggatgagg aactggttgc tggcggtcga gcggaaatcc gcgcccgcgc
    13921 tgtcggccca gacgaacgac ccctggtggt tcgccttggc ccggcggccc gcggcaaacg
    13981 cgaaatcgcc gccggcctcg ttctcccggc cgcccgggat cgcggcatag tcggcgtagt
    14041 tgtggatttt gttggaccag ccgccgccga tcacggagta ggcgcacagg gtggtgacgc
    14101 agttgcccgc gccgccgccg atcacggagc aataggactg ggtgtcgatg cggttgttca
    14161 ggccgccgcc gatcatggtc cagctcgcct ccgtcgcgat ccagttccgt tcgccgccgc
    14221 cgatgacgga ttcccgggcg ttggtgtcga cgaggttccc gcggccgccg ccgatgaccg
    14281 acgcggcgcc gaacacggcg ttcgtctcgc cgccgccgac ggcggagtgg acgcccgcct
    14341 ggacgttgcc gacgccctta aggccgggga tcaccgcgta cgccgccgag gccagccgct
    14401 cgcgcggcgc cagggccgcg ccgttgacca gcacgcgcac gtacacgttc gtgttgtcgt
    14461 tcaggatctg cgcgagcgtc ccggcgggca tgtcgtcgcc gaggaccgtg gcgtacagcc
    14521 cgtccacggc ggtcaccgtg ttcgagtcct cgcccagcat caggccgccc gtcgccgcgc
    14581 tccagagctg gagcgacagg ccgacctgtc cgttgtacag attcgtgccc tggatcaggc
    14641 gcccctgata gttcattacc gcggggacct gggcggagca cagcgcggag accaaggcaa
    14701 ggaccacaac cgacgcgagc aggcgagcgt tcatgatcag acctcctggg taggccgcca
    14761 ttataaaaag ggcggaagcc cgatgcaaca acgagacggc ggtcagggca gcatgccgcg
    14821 gacccggaac acggcggccg tggttcccgg cggcagcgcg tccaggttcg tcgcgatgcc
    14881 gcccgtgccc gcctcgtacc ctacgaggcc ggcggcccac ccgttggtga ccaggccggg
    14941 gaggtcggag tgctcgaccg cgtattgccg gtacgccgac gagggatagg tcaccaccgt
    15001 gacgcggtgg gtggggtagt ccaccgtcga ggcgcgcgcg atgcgcagga agctctctcc
    15061 gtcggtcggg tcggtgtccg cgtagaactc gtccaggttg ggcatcccgt catcgtcgcc
    15121 gtggccggcc ggatcgccgc ccgtagcgct gccgaagtag cgggcttccc agccgtccgg
    15181 catcccgtcc cggtcgctgt ccagcatggc gaagtgcgag aactcggagg tgtcgcccga
    15241 ggcgatgtgc gtcgccgtcg cggcgtagta gtcgggcacg ggatcgggcg ccggcacggt
    15301 ggcgttgaaa tgggcggtgt ccagcgaggt ccgcacgttg gtgctgccca ggctgatctg
    15361 gccctcgccg cacccggagg gatcgaactg cgtgttggcg tagaagttga tcttgtacgt
    15421 ctgggacacg ccgctgcgca ggaagccctg gaggcgaagg tcgacgccgt ccacgaatac
    15481 gttggtgatg accgggaagt tctggaggcc gttgggcccg tcgtcgccgt cgccgtacac
    15541 cggcccgcca tcgttgggcg taacgccgtc ggcgcccagg tcgatgccca ggccgccgtt
    15601 gtcgaaaatc cagttgtagt cgatccggat gccggtggtg ttggtgctgt tgcccagcac
    15661 gatgccgttc ctgccgttgt aggagatgat gttgatctcg gtccagacat cgccgccgat
    15721 gaggatgttc gtggaggccc cggtaatgac gatgccgtcg tccgtgttgc cgccgaactc
    15781 cgcgccctcg gcgtgggcgc cgatccggtt gcccagcagc acgcagtccg tgcagttgat
    15841 caatcggatg ccatcggtcc cgttggcgga aatcaggttc ccgccgccca cgagcaggtc
    15901 cccgccgacc cggcagcccg aaagggtatc cagccagagc ccctcccgcg cgttcggcag
    15961 cggatcttcg ccgtcttcat ccgtcccgat gaaattgctc tggaccacgt tgctgaccga
    16021 gagtgccccc gtgatgagga ttccggcagc cccgttgccg ctgaccacgt tgccggcggc
    16081 cgcggagacg ccgccgatcc ggttcgaggg cgcgtcgtcc acgtagactc cctcgtaccg
    16141 gttgcccagc gcgccctgcc cggaggccgt ggtgccgatc cagttgccct gcacggtcac
    16201 atcatgggcg ttggcgctgg tgatgtagat gccgtaccgg ccgttgcccg agaccacgtt
    16261 gccggcgccg atcccgccga tcgtgacaag cggcgcgccg gacaccatga tgcccgacca
    16321 gccgttggaa atcgccagcc cgcccgccgc ggtgaccccg acgtgattcc gctcgatcac
    16381 ggtgtcgtcc gacagctcgc cgaagatgaa gatgccgtgc cgcccgttgc cggagatgac
    16441 gttgcccgcg ccggcgttgg tgccgcccac cgtcgtgccc caggcatgct cgatatggat
    16501 cccgtggctt ccgttcccca gggcataacg gccggagacg tccgtgccga tgtagttgcc
    16561 cttgatagtg ttctgcgggg cataataaag gtttatgccg acgttcgtgt tcccggagat
    16621 gatgttgcgc gcggcggtca gggcgccgcc gatcgtgttg gactggcaga gtatctcctc
    16681 catgtagatc ccggtgtcgt tggggatggc attgctcccc gtgacgtccg tgccgatgta
    16741 gttgccctcg atccggttcc gcttgaccga ctgcgccagc ccgtccatcg tgatcccgtt
    16801 aaagcggttg ccggagatca ggttgcccga ggcgcctttt gttcccccga taaaattgtc
    16861 gttgccgttt atgaggatcc cttcgctgtt ggccaccgcg ccggtcccgg cggcgtccgt
    16921 cccgatgaag ttcccccgga tcgagttgct gctgctgatc cacgagatat cgatgccccg
    16981 caaccggttt cccgaaatga tgttgcgatc gtcggcgctc gggctcccga tgtagttctt
    17041 gtccgaggcg atgaaaatcc cgcgccagtt gccgtccgcg acgccggtca ggggatggat
    17101 cccgacataa ttggccagca ccgagttggt cgtgccctgc accaactgga tgccggtatc
    17161 gttcgtggcg aagtaattgc cgatgatctg ggtgccgccc gccttgatgc acaccccggc
    17221 atcggtgttg ctcatgaacc aggagcgctg gatcacgcag gagggcgagg tgaccatgat
    17281 gccggcctcg aagccgtgga cccgcatcgc tgtgaccgtg cagccgatcg cccccaggaa
    17341 cagcccgttc gtcaccacgg ccgacgccgc gcgcagcgtg accagcggct cgctgcccga
    17401 cgccaggtgg ttcgtgccgg tgatccggat ccggtccgtg atgcggggaa gctccgtgct
    17461 caccgcgatc gtccgcgagc cggccggcag gttgaatacg acgagatccg tgccggcgcc
    17521 gaggttcgca ttgctgatcg ccgcacgcag cgtgccgttc ccgctgttcg ccgagttggt
    17581 gacgatgtac gtggcgcctt cggccggacc ggaaaccacc gccgccgcgg cgacaagaag
    17641 cgcctttttg aaccaagtat tcatggctcc ctccctgcgg tagtcgctcc tgcttcacag
    17701 aatatcccat gtccccgccg cgagaaacgt tattccgcag cgcctttgtg cgggcaggga
    17761 ggcccggctt catccttcgc atgggatcgg gctccccgcg cgctcgatcc gccggacgtg
    17821 tttttcatgt gccgttgtgt tatgaatgga gcccaggcgt aggccggtcg tgaaaatact
    17881 tttatggttc ctgttccggg cggtattggc cgtcttctgc tttgtgtacg gcttcaagct
    17941 gcttttctgg ggccatccgg gcgcgggcct ggtcgggtgc ggggtcctgc tggccggcgc
    18001 ctgggtcatc gcgttcccca tcgcgcgcat cctggccgcg cccttctccg cgatcttctg
    18061 gccgaccggc gccgagccgc ccccgcccgt ctacggcatc ccggaatccc acgtgaagaa
    18121 agggctctat gccgaggcca tccgcgagta ccacgagatc atccggaaat ggccgcacga
    18181 gttaaaaccc tacgtggacc tgctcgacct cgccgtccgc cggatgcgcg acccggacct
    18241 ggccgcgcgc atccacggcg aggccctgct caagctgaag aagcccgagg agcgggaaac
    18301 cgtgacccgg ctgtacgaag ccatgaagga agcccggcat gaagaaggat gatctgcagg
    18361 cgattcccgg cgtcggcccg agcatcgcgg ccgaccttcg ctcgctgggc gtgacccgcg
    18421 tccgcgacct ggaaggccgc gacccgcagc ggctctacga cgccctcgtc cggcggaccg
    18481 gcgcgcccgt ggaccgctgt gtcctctatg tcttccgctg cgcggtctac ttcgcgggcc
    18541 attcgcggca tgacccggaa aaactgaaat ggtggaactg gaaggatccc cggtgaaacg
    18601 gatcctcgtg gcgattatcg gcagcggagc gaccgacaag ccggccctga tcgaggcggc
    18661 ccgcgcggcc ggggaggcca tcgcccggca tggggccgtc acggtggccg gggaatgcgg
    18721 cggcgtgttc ggcgcggcgt tcgccggggc gcgggcggcc ggcgggctga ccctggccct
    18781 gaccggcggc gacagggttc cggaggggca tcccgtcgcg gacgtggtgt tacctaccgg
    18841 cctgggaccg gcccggcacg ccgtgctggc cgcggcctgc tgcggtgccg tggtcatcga
    18901 gggcggaatg ccgaccctgg ccctggccgc cgagttcctg gctgcccgca agcccgtggt
    18961 ggtcgtcgcg gaaagcgggg gcgccgccgg cctgctggcc gggagcagtg cgctgcccgg
    19021 cgggacacgg gtactgtccg cgttcgacgg ccgcgccgcc gtggatctca tctttcaaat
    19081 cctgggagaa acaaacccat gaaatccatt caaaatgtcc tcgccgccgt ggtgctggcc
    19141 ctctggatgc tggtctcgct ctccctggcc accgccttct acatgtggat cggcgtgaag
    19201 gcgggccccg gcgcgctggg cgagatggag cgcgcgctcg gcgggatcac cctgttcgtc
    19261 caccccgggt tctgcttgcg catcctgtgg gcgcaggtcc tgttctgctt ccctgtcctg
    19321 ctcgcgatgg ccgtgcccgc cgcctggtgg ctgcggcggt ggtaccgaac ataaaaaaac
    19381 cctgccggga ctgcccggca gggttttcat gatcctcgcg gcgacttacg gcgtcacgat
    19441 gcggtagatc cgcagcatgt ccgcgggcgt cgggtcagac agtgtgatcg cgccgccgtc
    19501 gccgatcttc gtgtccgccg gcgtccagac cggcggtttc gccccgaggt cggtcgtgta
    19561 ctccagcgtg taactctgcc cgttgaccga cgtcagggtc gccgccgccg tcgcgcccgc
    19621 cgggaccgcg aagcccgtga tatccggctc ctcgaccggc aagccgttga ccgtgatcgt
    19681 gatcgtctcc tcgtccgatc cgtcgttgtc cgcggcgtta aacgtcaccg tgtaggtgcc
    19741 cgtcggcgag gcggcgtccc agacgaacga cgccagctca ttggtcgggt agaagaacgc
    19801 gcccgccggc aggttgctcg ccgtcagcgt cacggtgtcc gcctcggtcg ccgtcgccgt
    19861 cacggcgaag tgcaggttgc tgccgatccc gacggactgg tttccgatcg cgttcagcac
    19921 cggcggcgtc ccggacggag ccgccgtcag cacgttggtc gtcgccgagt tcgcggtggt
    19981 ggtcgtcgcg ttgtacgccc ggacgcggta gtagtacatc acgtccaccg ccagccccgt
    20041 gaccccgtac gtcgtcacat tcgcgacgtc ccggttctcg tagccgggca cgtagtcgtt
    20101 gaccccgccg ccactgctca ccgtgaagtc cggtgtgaac ttggcgtagg ccgtggcgtc
    20161 gctgccgctc cagttcgccg cgttcgctac cagctgcagc agcgccgact tcgtgcccgt
    20221 cgtgccgctg gccgagttgt attgctgatt gtcgaaactg ccgcatgaca cgatggtgaa
    20281 cgcgccggtg ctcaagctcg acgggatgca ggaattgttg gctgtgaggg ccgtgccgtc
    20341 ggcaacccat ggcgaggcga tcgcccagcc agcgccgtac aagaactgcg ggctttcagc
    20401 gctgccctga taggccagga tggtgtcgcc ggccgccgcc agggcgaagg aggcgccggt
    20461 tttcacaaac ccgttttcat ccacggagcg gtaggacaac acggtccccg ccgtgaccgc
    20521 gcccggcgcg gtgtacgtga cgatgccttc ggaggcccgc cacccgttgc tccagccgtt
    20581 gtcggtgaag tagatcacgg tgccggcgtc caggtccacc agcggcacga cgtcaaagcc
    20641 ttccggatca tcggcgtcta ccgtcacgat catcaggtcg cccgggctca acgacgagac
    20701 cttgggcagg cgcgccgtgt ggcggaactg gttgttcgtg gccacgtcca gccggtagcc
    20761 ggtcgcgccg gccgaggcca gccagttggc gtcgaactgg ttggtatcga cgttgctggc
    20821 cgcctggacg atcggcggca gcagcacctc ggcgccgccg gtgacgatga cctgcaccgc
    20881 gagcgaattg gtgccgtcct tgtcgccggc gtagaaggtc acgacgttcg tgctgcccgc
    20941 cggcggcgtg ccgctgaagg tgttggtcac ccccccggcg ttggtcaccg tggcgaacgt
    21001 cgcccacgcg gggacgccct ccgcccacag ggtcacgtcg tccgcgtccg gcgcggtcga
    21061 ggcggacacg acgaagctca acaggttggt gatcgtcccc gcctggttcg tgctcgccgg
    21121 cgccgtaaac gtcggcggga tgtccccggc cgccgtggtc agcacgttgg tcacggccga
    21181 gttgacgctg ttggacgtca ggttgtaggc ccggacgcgg tagtagtaca tgacgtccac
    21241 cgccagcccc gtgaccccgt aggtcgtcac gttgacaacg tcccggtcct cgtagcccgg
    21301 cacgtagtcg ttgaccccgc cgccactgct caccgtgaag tccggcgtga acttggcgta
    21361 ggccgtggtg tcattgccgg cccagttcgc cgccgtcccc gtcttctgca gcagttccgc
    21421 cttcgtcccg ctcgtgccgt tggccgagct gtactggtag ttgtccgagg tcccgcaggc
    21481 cacgatggtg tacgcccccg tgctcaagcc cgaaggcatc tcggaggtgt tcgccgtcag
    21541 caccgtgccc gcggtcaccc acggcgaggc gatcgcccac ccgatgccgt acaggaactg
    21601 cgggctgccc gcgctgccct ggtaggccag aatggtgtcg ccggaggccg acagatcgaa
    21661 gctgcccgac ttgacgaacc cgttctcatc cacggaccgg tacgagagca ccgtgcccgc
    21721 cgtgatcgcg cccggcgcgg tataggtgac ggtgccttcg gatgcgcgcc atccgttgct
    21781 ccagccgttg tcggtgaagt agatcaccgt gccggcgtcc agatccacca gcggcacgac
    21841 gtcaaagcct tccgtggcgt cggcgtccgc cgtcaccatc atcaggtcgc ccgggctcaa
    21901 cgacgagacc ttgggcagcc gcgccgtgtg acggaactgg ttgttcgtcg ccacatccag
    21961 ccggtagccg gtcgcgccgg ctgaggcctg ccagttcgcg tcgaattggt tggcctgcac
    22021 gccgctggcg gcgaggatga cgggcggcag cagcacctcg gcgccgccgg tgacgacgat
    22081 ctgcaccgcg cgggagttgg tgccgtcctt gtcgccggcg tagaaggtca cgacgttcgt
    22141 gctgcccgcc ggcggcgtgc cgctgaaggt gttggtcacc cccccggcgt tggtcaccgt
    22201 ggcgaacgtc gcccacgcgg ggacgccctc cgcccacagg gtcacgtcgt ccgcgtccgg
    22261 cgcggtcgag gcggacacga cgaagctcaa caggttggtg atcgtccccg cctggttcgt
    22321 gctcgccggc gccgtgaacg tcggcggctg gtcgggcgcg gccgtggtca ggacgttggt
    22381 gacgccggaa taggcgctgt tggacgtggc gtggtacgcc atgacccggt agtagtacat
    22441 cacgttctcc gtcaggcccg tgacgggctc ggtcgtgacg tcgcccacgt cgcgcgcctg
    22501 gtagccgagg acgtacgagc cgcccgccgc gccctgcgcg atcgcgaagt tgtccaggta
    22561 gaaccggttg ttggagatgc ccttggccgt gatccggatc ttcgacgccg ccgtgccgcc
    22621 ggtgagctcc acggtctgca gcgccatgga ggccggcggg atcaggtcgg agccgacctg
    22681 cacgaacgtc gagccgtccg ccgcgtgcat gacctgcacc gcgttgacgt cggtcccgta
    22741 cttgccgagg tcgaacgtca ccgtcgccgc gccgccgttg ccggacaggt tcaccgtggg
    22801 cgtcgtgatg tagcccttgg cggaactgga gccgatcttc gccgtgccga tgttttcgta
    22861 gactttcgtc ccagtccacc cggccgtatg cgtgtacgtg tccaggctgc cgccgatgtc
    22921 cgcgctgctg ttggtccggg tgaagagcgc gaagtcttcc gcgaggttcg tggtcgcgcc
    22981 gccgctcccg gtgaacgagc tgttggtgga cacgtccagc cggtagcccg ccgcgttggc
    23041 cgaggccagc cagttggcgt tgaactgcgt gccctgcacg cccgtcgcgg cctggatgac
    23101 cggcgggagc agctcgggcg ccggctcgtt gacgacgatg gtgacgccct gcgtcaccga
    23161 gacctgcgcg tcgaacgcga cgaacaccgc cgtaaacgtc cccgtttccg ccggtgtcca
    23221 cgtgaacgtg ctctggatcg gcgccgtgcc gctcaccgtc gggaagttcg cgccggccgg
    23281 cgcggcgctg gtgtacacgc ccaccgactc ggcgtccgcg tcgttgaccg tcaccacgaa
    23341 ggtcagctcc gagcccgttg tcacggtctg gttcgtcccg cccgccgcgc tgatcgtcgg
    23401 ctcccggttg accggcgagc cgcctccgca cccggcgatg atcacgtcgt caaaagccag
    23461 gttgccggcc gacttgtcgt aggtgaagcg caactgcgaa atgccctccg cgatcgccag
    23521 cgactgcgtg gtcgcagtcg tcaatatcgg gacaatcgtc cccaccgtgc tccagctcga
    23581 cccgttgtag ccttccacca gcaggaagga gctggcgtcc gtgccctgtc ccttgatcca
    23641 gaacgtgacg ttgctgggca tgttcagcgc aggggtttcc acgcggtcgc cgtcgaggtc
    23701 aaacttgagg gagggcgacg cccggccgta gttgcccgcg gtggcgtacg tcccgccgat
    23761 gctcgtgaag gtccatccgg ccggcgcggt ggtccctccg tcgaacccct cgtcgatgat
    23821 gttggtcggg atgcagtaga tgtcgttggc gacgacggtg atcgtctcgg aattggtgcc
    23881 gtccttgtcg gcggcgtaga aggtgaccgc gtacgtgccg gtgaccagcg gcgtgccgga
    23941 gaattggctg ctgacggtcg aggcgttggt cgcgccggca aacgtcgccc acggcggcag
    24001 gccctccgcc cacaggcgga tggagtcgtt gtcgatcgtg tcgcttgcgg agacggtgaa
    24061 ctggagcagg ttgttggtcg tgacggtctt gtggccgacc gggtcgaggc tcggcgggac
    24121 gttggtgggg acgaaggccg tcgcgttggc gacgaccagc gcgaagtcct ggtccagcgc
    24181 gccgccgtag ctcgggacgc cgtcggagtt gatactggtc gcgctgacgg tcacctggac
    24241 gagcccggtc gtgccggcgc ggagaaacac gctctcgctg ttgttgcggg cgtccgccgt
    24301 cccgccgttg gtcgagtagg cgccgctgaa gacgttgccc ttgtaggtca ctccgccggc
    24361 gacgacggtg aggtcgaggt tgttgttgta cgcgttgccc gaggtcgagc cgggggcgtc
    24421 ggtccagccc actgtgacgc gcacgggcag gccggtgttc gacacctggc cgtagaaccg
    24481 ccgcgactgg ccgctggcgg tgaagaggtc gttggtcagc tggtcgcgca ggatgcgggc
    24541 cgcgccgtcg aaggccgtgc cgaggttcat catgcccatg ccctggttat tggagggcag
    24601 ggtatcgccg gtgtccacgc cgtccaggta ccgggcggcg ttcatgaggt aggccttgac
    24661 catggcgggg ctgggcacgg tccagccctg gttgatgaag tgctgccgga ccagcgcgca
    24721 gccgccggcg accgcgggcg tcgagtggct ggtgccgctg gaggccgcgt agttcgtctg
    24781 gccggcgggc tggtacttgt cgcacacgcc gttgcccgtg tagccggagt agaagctcgc
    24841 ggcgccctgg acgtgcgtgc cgggggcgac gatgtccggc ttcttgcgct ggtcgacgca
    24901 cgggccgcgg ctggagaaat cgatgatgtc gttcgcgttg tccgcgcccg tatcatccac
    24961 ggcgcagccg tccgtcccga acatgttgta gttctcggcc gcgccgacgg agatgatgtt
    25021 cttcgccgtg ccgggcgcgc cgatggtcgt gctggtcggc ccgtcgttgc cggcggcgaa
    25081 gacgaacagg cattcctggt tgccggccac gccgggctgc gcgtcgcggg tcaccgtgtc
    25141 gtagttctgc gcgtacacgt cgtacacgcc gtcgcccggc atgccccagc tgtcggagct
    25201 gatgcgcacg cccttgttgt agttggagct gatgtactgc agttcctgct cgtccgtcgg
    25261 ggcccagtcg cccgcgtcgt cgaacacctt cgtgttgccc aggttcgcaa aggggcacac
    25321 gccgaggccc cggttatagc ccgaggcgtc ctcgtacggg ctgccggtca ggtcgttgta
    25381 gccgccgacg atgctgatgt tgatgttgcc gtgcccgtcc gtgccccacg atccggccga
    25441 ggccagcgtg ctgccccact gcgagtactt catgcggctg ggttgagccg cgctgccgct
    25501 cacgcggaac tccgggttgg ccggggtggc ggccacgccg ttatcccacc cgtcgtcggc
    25561 gatatccacg atgaaccccg acgcgtcgaa ctgcgtctgg gtgaagcctt tggagtacag
    25621 ccaggtcagg tagccggtcc ccgacggctg gctgcccgcg acgttcaggt tgcccgcgac
    25681 gatctggtcc tgccgctcgc agaagggccg cggcgggaag tacgggtgga tcgaaaccac
    25741 gtccggccgc gccgcgaggt ctgccaggcg gtcggcgggg agggcgaccg tgacgttcag
    25801 gaagcccagc cgcgcgttct cgctgcggct gcggatgggc gcgagcttga ggctgtccac
    25861 cagcgccagc gtctccgcgt tggcctccgg gtccgcgacc atctggatcg aaaacagttc
    25921 cgtcccgatc gcgcgggcct tgccctgcgc gtccgtcgtc cgcgcgccgg gctggatctt
    25981 gtccgtgtcg gcgtacgcgc cttcccactg gatgtatgcc cggcgggcct ggttggcctg
    26041 gagccgcgtc acgccgtccg agtcggaata gacgaggtac gcgttgtccg ggatgtacga
    26101 gacgatccgc gcgccggtcg ccgccagctc ctcgtgccat tccggcttga ccgggcccgc
    26161 gaactggacc aggtgcaggc gcttgcccgt gaaccccgtc gccaggcgcc gagccgagcg
    26221 cacctcggcg gacgacgggt ccaccgcgcc ggcgttcagc cggatctggt catccagcgc
    26281 ctcggccgcg tcgcccagcg ccgcgaccgc cgccgcgtcg gcttccatga cggagaagga
    26341 ttcgtattcg gcgatcaacg tcccgccggc gtcggtcagc tgccgcacgg cgtccgcccc
    26401 cgtgacgcgg accttcaccg ccgccgccgg aaagaccatg gaaaccaaga agcaaaaact
    26461 cgccgcgatc ctgatcactt tcatgctcgc ctcgctggta ggttatgcgg acaactcatc
    26521 gaaataatgg gggtggagag tacccccaac gcaacgcgaa ggaaagagca attccttgcc
    26581 gcgtaaaaag ttgtggaaca cgaagatgat aaatcgtttt actaaacgaa aatagagccg
    26641 acaaggaaca aggcgtcagg gaattcgggc ccacccgctc tgcctctctg ccactttgcc
    26701 gctctgtcac tttgccactc cgcgtgctac tctctgcccg catggacacc gccgacttta
    26761 gccacctgac gcccgaggcc gtcatcaacg ccgtcgaagc gacgctcggc gttccctgca
    26821 cgaacatctg caggccgctc aacagctata tcaaccgcgt gtacgaggtc ggccttgacg
    26881 atggcggata cgtgatcgcc aagttctacc ggccgggccg ttgggaccgc gtggcgcttc
    26941 aggatgaatt ggatttcctg gccgagctcg cggaggagga ggtccccgtc gtgccgccgc
    27001 tccccggtcg cgacggggcc ctgctccacg acctcgcgca cacggccttc gcgatttttc
    27061 cgaagcgcgg cggccggccg ctcgacgaac ccgacgcgaa cacgtggacg cagctcggcc
    27121 gcctcctcgc gcgtatgcac gcggtcggcg cgcggcggct cccgaaaaac cgcgtcacgt
    27181 tgcacccgga gcattccacg cggcagcacc tagacttcat cctccggtcc ggcacgatcc
    27241 cctccggtcc ggctcgcgcg tacgagcggc tcgtaacgga actcatcgag ctggtagccc
    27301 cggatttcga gggcgtggag acgcaccgca tccacgggga ctgccaccgc cagaacatcc
    27361 tgcaccgccc cggcgagggg ttccacctgc tggacttcga cgacatggcc gtcgggcccg
    27421 ccgtgcagga cctgtggatg ctgctgcccg atcgcgtgcg cgccgcgcgc gtcgaactgg
    27481 aaagcctgct ggagggttac gagaccttcc tgccgtttcc gcgggagacg ctgggcctgg
    27541 tcgagccgtt gcgcgcgatg cggtttatcc actacaccgc gtggtgcgcg cggcagaagg
    27601 cggatggcgg cttcgcccgc ctcgcgcccg actggggctc gcaggcgttc tggcgccagg
    27661 agaccgccga tctcgagcgc cagcggcagg agatcctcga cgcgcgctag cctggataat
    27721 gcgcgactgg cgcgcatgat ccgcccaaag ggcctccacg tgagccgcgt aggccgcggc
    27781 aacgtggccg caggcggtga tgtgcccgcg ttgcgggcac aaacggggct tgccacgttc
    27841 gttttgtcga atccctgatt cgacaaaacg tcacgtcttc tggcacgcga attgcccatg
    27901 caacgcgcgt gctcaaagcg cttattgcgt gagcgtcact atgctcatga cataagcggg
    27961 ctagcgccga atacacccat cgctggctag tccggcgggc gtgggcagcg cgggtcgcag
    28021 cgcgggcaag gcctcccctt gtgtgctggc aggacatgcg tgccgggatt gcgaccttcg
    28081 tgaggtgtct tgcgattctg aagttaatat cacactttgc aaagtgtgga atttagtaac
    28141 acgaaatgga actggtgatt agtaaataat caatattgtt atcttgagca atttccacac
    28201 tttgcaaagt gtgatgttta gagatgggtg aatacaaagg gatggccgcc tgccatggcg
    28261 gaccaggcca ggcgagccgc atcggcgcgg gtggcccctc ctcgttcgtc gggaggcagg
    28321 atggccgtgc ttttcgtggg tgtacgcaaa ggaacgtata cgttttcatg ccgttatgta
    28381 aaaatatcgc cgaatgagac tcctgttctc catgctgctg gtcggcgcgg gcggattcgt
    28441 cggctcggtc ctgcgctacg cgctcgcgct cgcggcgcag cgctactcca tctccttccc
    28501 gcacggcacg ctgtgggcca acctgctcgg ctgcctcgcc atcggagcga tcagcgcctt
    28561 ggcggcggcc accgaggtct tgagccccgc cgtccggctc ctgctggcca cggggatatg
    28621 cggcggcttc acgacgatgt cgtccttcat ctacgagctg gcccagctcc tgcgggataa
    28681 cgagctgtgg ctggcggcgg gatatttcgg gctgacgctg ggcgggtgcc tggcgatgtt
    28741 ctggatcggg gcgctggccg tgaaactgct gctgaaagcg taggggcacc atggaactca
    28801 agggcgaggc gaaactgctg cgaatattcc tgggcgagtc ggataaggtc cggcacacgc
    28861 cgctgtacga ggcgatcgtc aaggaggcgc gctcggccgg actcgccggc gcgacggcgt
    28921 ggcgcggcat cctgggctac ggcccgacca gccggatccg gtcggccaag atcctggacc
    28981 tgtccagcga cctgcccgtt gtcatcgaga tcaacgacga cgaggaaaag atcaaccgtt
    29041 tcctgccgaa gctgcacgag ttgttcgaaa cggcgcagag cggcggcctg atcaccatcg
    29101 agaaggtcca ggtcatcaag tacctgcacg ggaacccggc gtgaaaaccg gcgcggagat
    29161 ggagaggaaa tccgaaagag catgacgcgc gaaatcgaac tgaccgccga gaagatggtc
    29221 gccgagggcc gggcgctggc ccggccggac gggttcgtcg tgttcgtcgg cggcgcgctg
    29281 ccgggcgagc gcgtccgggc gcgcatcacg cagcgcaaga aatccttcgc cttcgcgcag
    29341 gcgctggaga tcctcgatcc gtcgcccgac cggcaggctc cgccgtgccc cgttttcggc
    29401 cagtgcggcg gctgcgcctt ccagcacatg gcctacccgg cccaattgcg cgcgaagcac
    29461 cagatcctga tcgaggccct ttacggcctg cccggcgtgc cgggcatcgt tgccgagccg
    29521 ttgggcatgg aagagccggt ccacttccgc aataagatgg gctttgcgtt cggcatggcg
    29581 gaggggcggg ccgtcgtggg cctgcaccgg cgcggggact ggcggtccgt ggtccctgcc
    29641 ggaacctgcc tgctccagtc gcccgaatcc cgcgagatcc tcgcccgcgt cctggccttc
    29701 gtgcaggacc agcgcctgcc cgtgtacgac gaggagaagc agcaggggct cgttcgccac
    29761 ctcgtcgtcc gcgagggcaa gcacacgggc gagcgcatgg ttcacctgca cgcggccgaa
    29821 cgccacccgg cgttcgagca gttgcccgcc gtcctcggcg acttcgcgtc caccgtgctc
    29881 gcaagttttc acacccaggt tcccgagtcc gccccgccgg gctcgaccac ggttctgtcc
    29941 ggcccgggac tgatccacga gcgcctgaac ggcttcacct tcgagatcgg gcccgcgacg
    30001 ttcttccaga ccaacacgcg gcagggcgag cggctgttcg ggctcgttcg cgactgggcc
    30061 gcggactgcc ggccggagcg ggcggtggac ctctatgccg ggaccggacc catcgccatt
    30121 cacctggctg ccgccgcgtc gcaagtgctt ggcatagaat cccatgcgcc gtccgtggac
    30181 gcggcgcggc ggaacgtgga gctcaacatt ctttccaacg tgcggatggt gtgcgccgag
    30241 gccgagcggg tgggggagac gctgcgcgag gaacgcgcgg acctcgtggt cgtggacccg
    30301 ccgcggccgg ggcttcatcc gaaagcgaag gcggccctgc tgtcggccgc gccgccgaac
    30361 ctgatctacg tctcctgcaa cccggccacg ctggcgcgcg acctggcgaa gcttgtcgag
    30421 tccggctacg cgctggagcg ggtgcagccg ctggacatgt tcccgcacac gttccacatc
    30481 gaggccgtgg cgctattgag cagaaaaatg aatgcccaac atccaaccgc caccgcccat
    30541 cgcccagggg cttggtagtt ggaagtcggt tgttggatgt tgagtcaata aaagcgccgg
    30601 ggtcggcagt ggaggatgcc ggaccccggc gcggtggacg ggaatcttat gcctgactac
    30661 tgggccgcgg gcgcatcctc caattgcacg ccggcatcgg tgagctgctt gacgtacttg
    30721 tccggatcgg ccttgaacgt cgatacacag gcgttgcagc acaggtagac gcgcttgccg
    30781 ttgtgatcca cgtagatgga cttgttgacc gctccgccca tgaccgggca cgcggtctgc
    30841 gccttgacct cgcccgccgg ctggacggct ggcgacggct gggctgacgg cgccgccgcg
    30901 gcttccgcct tttcgacctt ggctccgcat ccgccggcgg atttcgagcc ggccgcggct
    30961 gcgccgcagg cgccgcccgc ccgggcggat aggctggccg cgacgaagcc cgcgaccgac
    31021 atcagcagca tcattttttt catcatactt gtctcctttg caggggaaca tacgacacct
    31081 ttttctcaat gcaaccgacc cccgcccgtt cgcattaaac gcaactattt tgtcaagaaa
    31141 taacatccgc tgtaaaggcc cgctttttgg gcgatttttc gagttttctc gcgggttttc
    31201 gtttgttttt ttattgcgcg agcccccccc gtttggatat attcttgaag ctttttcctg
    31261 gagcgttttt ccatggcaaa gaagtcagaa aaagtattgt ctgccccggc cccggaggag
    31321 gtccagccgg ccgctcccgc cttggccgag ccggccgcgg cgggtgcccc ggaaccggcc
    31381 aagcggccgg ccggcggggc gaagtacgac gccaaatcca tcacggtcct cggcggcatg
    31441 gacgccgtcc gcaagcggcc cgcgatgtac atcggcgaca ccggcgcgcg cggcctgcac
    31501 cactgcgtgt tcgaggtcgt ggacaacagc gtggacgagg cgctcgcggg ctactgccag
    31561 cgcatcgtgg tcacgatgaa ctccgacggg tcggtcagcg tcctggacga cgggcgcggg
    31621 atcccggtgg acatgcacgc cacggagaag cggcccgccc tcgaggtcgt catgacggtc
    31681 ctgcacgcgg gcggcaagtt cgaccacgac agctacaagg tctccggcgg cctgcacggg
    31741 gtcggcgtgt cgtgcgtcaa cgcactgagc gaatggatgg aggtcgaggt tcgccgcgac
    31801 ggcggcgtgt accaccagcg gtacgagcgc ggcaaggccg tctcgaacgt ggaggccatc
    31861 ggcaagacga aaagctccgg cacgaaggtc accttctatc ccgaccacaa gatattcccg
    31921 tcgctcgagt tcagctggga tatcctggcg aaccgcctgc gcgagctggc gttcctgaac
    31981 aagggcatcg agatcacgct ccagcaggag gacccggcgc gcgaggagac cttcaagtac
    32041 aagggcggca tcgtcgagtt cgtcgagcac ctgaacaaga acaagaaccc gctgcacccg
    32101 aaggtggtgt tcttcgagaa ggagaaggac cgcatccagg tcgagatcgc gctgcagtac
    32161 agcgacgcct tcaacgagaa tatcttcacg tacaccaaca acatccacac gatcgagggc
    32221 ggcacgcact tgagcggatt ccgctctgcc ctgacccgcg tgatcaacca gtacgccaag
    32281 gccaacaagc tgctcaaggg cgacgacgag acgatgagcg gcgacgacgt gcgcgaaggc
    32341 ctgacgtgcg tcatcagcgt caaggtgccg gacccgcagt tcgaggggca gacgaagacc
    32401 aagctgggga acagcgaggt cgagggcatc gtggcgtcga tcgtcaacga ggaactgggc
    32461 acctacttcg aggagcatcc gtccgtggcg cggcggatca tcgagaaggg ggtgctggcg
    32521 gcgcgggcgc gcgaggcggc ccgcaaggcc cgtgacctga cgcgccgcaa gggcgcgctg
    32581 gacagcgggg ggctgccggg caagctggcg gactgctcgg agaaggaccc ggagctgtgc
    32641 gagctgttca tcgtcgaggg cgacagcgcc ggcggttcgg ccaagcaggg ccgcgaccgg
    32701 cggttccagg ccatcctgcc cctgcgcggc aaggtgctga acgtcgagaa ggcccgcctg
    32761 gacaagatgc tgaacaacaa cgaaatccgg accctggcca ccgcgattgg cgcgggcatc
    32821 ggcaaggacg agtttgacgt ggccaaggcc cgctaccaca aggtcatcat catgaccgat
    32881 gccgatgtgg acggtctcca catc
//
LOCUS       DAOOTK010000025        31073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1062_length_31073_cov_6.305661, whole genome shotgun sequence.
ACCESSION   DAOOTK010000025 DAOOTK010000000
VERSION     DAOOTK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 31073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31073
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_31073_cov_6.305661"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..626
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..32
                     /rpt_unit_seq="gtttcaatccacgcccccgcgcggggggcgac"
     gene            complement(813..1106)
                     /gene="cas2"
                     /locus_tag="PK634_04790"
     CDS             complement(813..1106)
                     /gene="cas2"
                     /locus_tag="PK634_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOW97086.1"
                     /translation="MLVLVSYDVSTVDNRGKARLRRMAKACLDYGQRVQNSVFECNVD
                     PAQWAILNNRLLDICKEDEDSLRFYYLGSNWRRRVEHYGNKKIADLEEGLLVV"
     gene            complement(1109..2140)
                     /gene="cas1c"
                     /locus_tag="PK634_04795"
     CDS             complement(1109..2140)
                     /gene="cas1c"
                     /locus_tag="PK634_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOW97087.1"
                     /translation="MKKLLNTLFVTTQGAYLCHEGESVLVRVENEVRLRVPIHTIGGI
                     VCFGLVSCSAPLMGLCGERGVRITHLSERGRFHARVEGPVSGNVLLRREQYRRADQPE
                     AASALASSIVIGKIANSRTVLQRAARDHEGKGGMDDIERVVARLGSLLKELENDIPLD
                     NLRGREGEAANLYFGVFDHLITAQKEDFKFVARSRRPPLDNINALLSFLYTLLVHDAS
                     AALETVGLDPQVGYLHALRPGRPALALDMIEEFRPFLADRLALSLVNLRQVQAGGFVK
                     SESGAVVMDDETRKALLLAYQKRKQEEIYHPFIEEDVPVGLLFHVQALLMARHLRGDL
                     DAYPPFLWR"
     gene            complement(2124..2762)
                     /gene="cas4"
                     /locus_tag="PK634_04800"
     CDS             complement(2124..2762)
                     /gene="cas4"
                     /locus_tag="PK634_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOW97088.1"
                     /translation="MYSEDDLLPLSALQHLMFCERQCALIHLEGVWDENRLTAEGRIL
                     HEKVHEGGDETRGDVRIARGLRLRSLRLGITGQADVVEFHRQADGSWQPFPVEYKRGR
                     PKPDTCDEVQLCAQAFCLEEMLGAQIPEGAIFYGQPRRRHGAVFSAELRHQTEASAAR
                     LHDLLDSGLTPRAVYEKKCDNCSLKLSCLPEVAGRGKSAKRYLGQMLHEETA"
     gene            complement(2763..3272)
                     /locus_tag="PK634_04805"
     CDS             complement(2763..3272)
                     /locus_tag="PK634_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOW97089.1"
                     /translation="MIYLLRGQRVMLDSDLATLYGVKTKELNKAVSRNRDRFPADFVF
                     PLAREEVARLRFQIGTSNEGRGGRRYRPYAFTEEGVAMLSGVLRSERAVHVNVAIMRA
                     FVRLRGLLAAHHELAAKLTELERRVASNEEGIQTLFEAIRQLMSPPEPTRKQIGFSVR
                     EKRAHYGRK"
     gene            complement(3323..4189)
                     /gene="cas7c"
                     /locus_tag="PK634_04810"
     CDS             complement(3323..4189)
                     /gene="cas7c"
                     /locus_tag="PK634_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOW97090.1"
                     /translation="MTEAIKNRFEFVYLFDVENGNPNGDPDAGNMPRIDPETGHGIVT
                     DVCLKRKVRNYVQLAKDDEAPFMIYVKEKAVLSTSRAPAYKATEVEGDEEKRIAAARA
                     WMCEHFYDVRTFGAVMSTKVNNCGQVRGPVQFSFGRSVEPIAGLELSITRMAVETEQE
                     AKDQAGDNRTMGRKHAVPYALYRSEGFISAPLAAQTGFSEDDLELLWQALQNMFEHDH
                     SAARGKMAARKLIVFKHADKLGKAPAQALFDLVGVERSGDVSKPARAYSDYKVRVNKA
                     GVPAGVELIEML"
     gene            complement(4193..5926)
                     /gene="cas8c"
                     /locus_tag="PK634_04815"
     CDS             complement(4193..5926)
                     /gene="cas8c"
                     /locus_tag="PK634_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOW97091.1"
                     /translation="MILQALARYYGRLASDSSSGIAPPGYCAQGVTTSLVLGHDGQIL
                     QVRDIRITGKGKPRPALLVIPARPTGRTGASIKPGFLGDETGYALGDDGKGKAERAAE
                     KFDSFRKLHRDLLAGVDDPAAEAMLRFLDTWEAEKAAALDRWMEWVGTTVVFEYADEG
                     YLHNRERLKRAWEHMPQTKDSTGGVCLVTGNPSPIARLHPSIKGVRNAQTTGAALVSF
                     NFDAVCSYGKEQSFNAPVSESAAFEYTTALNHLLKQGHQRIQLADATALYWTAQASKA
                     ESFMGMVFETSDDTAELARLREWLEAVRSGRFPKDLDPSVPFYMLGLAAPAKARLSVR
                     FWYAGTVGEMGERLKRHFEDLAIKRRFENEPEYPALDRLLPETAVLHEAKNIPETLAD
                     AMMRSVLSGGRYPASLLPTLLARAHAEQAAKTKRGKPEANVSYFRAALIKAVLVRNYN
                     MEVSMSLNPDCREPGYLLGRLFAVLERTQEYANPGINATIRDRYYGSASATPAAVFPV
                     LVRLNQHHLSKLSGDKKGLAVTMENLNADIVGGLPERFPPRLNLEQQGMFALGYYHQR
                     LAKKQGTKEEK"
     gene            complement(5923..6570)
                     /gene="cas5c"
                     /locus_tag="PK634_04820"
     CDS             complement(5923..6570)
                     /gene="cas5c"
                     /locus_tag="PK634_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOW97092.1"
                     /translation="MSYGVRVRVRGDYACFTRPEMKAERVSYDVMTPSAARGVLEAIY
                     WKPEIRWAVDKIHVLKPIRFTSVRRNEVSDKASVDNMLTAMRRGQGSLALFVEDKRQQ
                     RAAMILRDVEYVIEAHFEIVSGERNEGKHLDQFNRRVKDGACYHRPYLGCREFPALFE
                     PVAEIPASPVKGERDLGFMLHDIDHANDRRPHFFDAKLVDGVLDVPPFTPAEVKP"
     gene            complement(6567..8696)
                     /locus_tag="PK634_04825"
     CDS             complement(6567..8696)
                     /locus_tag="PK634_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOW97093.1"
                     /translation="MEDHLKCTAELAAKFAKPFGAEDWAYLAGLWHDLGKYSEAFQRR
                     LNGSTEHVDHSTAGAQHAVAGDIDPQAGKLIAYVIAGHHGGLPNGSSAVSSDLQARLR
                     KVVEDFSLVPPDILSPKTLPRQLSWLDKDRYLYQIAFFVRMLFSCLVDADFLNTEAFM
                     KPEQAKARGGLPDIGRMEKRLFRYLEMLPAKGSAAVSHARRRVLHDCVQASSWPPSFF
                     SLTVPTGGGKTLSSLAFALRHARTHGLGRVIYALPFTSIIEQNADVFRKALGDCADGV
                     IEHHSNLDPDERDDVAKLATENWDAPVVVTTNVQLFESIHGNRVSACRKLHNLARSVI
                     ILDEAQALPVALLEPCLEALRELVADYGATVLLCTATQPALNRCAQFPKGIDHVHEII
                     PDPSALAAELKRVNVRVLGKQTDEEVGEALRKHPRALCIVNTRRHARKLHGLLAGDDC
                     FHLSALMCPTHRRAVIATIRERLATPNAACRVVSTQLIEAGVDVDFPVVFRAMAGLDS
                     IAQAAGRCNREGRLDRGETFVFQGEKPPPAGLLRQAAQAAELVLPLFADDPLSLDAVR
                     AYFERHYWTQSDRMDVPGILRRTREFDLKTLWIPFEGIAREFRMIRDDGWPVLVPYDE
                     EARGLIAALRRDGPSGGLLRKLQRFSVNVYAQDLAKLGSAVEQIHERYFVLIRDDAYD
                     PRTGLNVEVSEVFEPEALYFDGKEKRG"
     gene            complement(8840..11320)
                     /locus_tag="PK634_04830"
     CDS             complement(8840..11320)
                     /locus_tag="PK634_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOW97094.1"
                     /translation="MNNFTPRAQQVVQLARKEADRFNHGYVGTEHLLLGLVALGQGVA
                     VNVLRKMGIDLETVRLEVEKAVGVGPATKTAGNVPFTPRMKKVLALAGSEARALNHAY
                     VGTEHLLLGLLREGEGAAARVLKNLEVDLEKTRVEVMKELDPGYDPSMEPPPGEPSGG
                     PAGPEGKPAAAKDSKTPALSTFGRDLTDLANKGQLDPVIGRANEIERVIQILCRRTKN
                     NPVLLGEAGVGKTAIVEGLAQAITRGDVPELLRGKRVVTLDLALMVAGTKYRGQFEER
                     IKAVMDEIRRNKNVILFIDELHSIVGAGSAEGAMDASNIIKPALSRGELQCVGATTLA
                     EYRKFIEKDAALERRFQSITVREPTIEEAVEILRGLRPRYEEHHHAKITDEAIVEAVH
                     LSSRYLTSRYLPDKAIDVMDEAGARGRISAMTRPPDLKEQEKKIEEIRKQKEEAIKLQ
                     QFEQAAAFRDQERQAREEADKAMEAWRKEQDGKIVTVDAEDIRHIVSKWTGVPLTKMA
                     DLESKKLLQMEANIRKTVIGQDAAVAAISKALRRSRAELKDPRRPIGSFILLGPTGVG
                     KTLLAKALAEFMFDDPDALIQIDMSEYMEKFNVSRMVGSPPGYVGYEEGGQLTEKVRR
                     RPYSVVLFDEIEKAHPDTMNILLQILEEGKLTDSLGRSVDFRNTVVLMTSNLGSEFVR
                     KGGAMGFSRTDAAADYDALKRQMLDEAKRVFRPELLNRVEEVIVFRQLGIEDMEKILD
                     LEVAKIRKRLAAKNIELHLSDPARKFLIGKGFDPLLGARPMRRAVEKYLEDPLAEELL
                     SGHLAGSEAIEVTADGDALKFGQLAGAS"
     gene            complement(11331..12395)
                     /locus_tag="PK634_04835"
     CDS             complement(11331..12395)
                     /locus_tag="PK634_04835"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOW97095.1"
                     /translation="MNVDDLIGRPAAWLDAGGGSDPVVSSRIRLARNLHNAAFPGWAG
                     EEECEKIWQSLRPTLQAAPTLSGSYAVGMEELDELDRQILFERHLISHEHAGRGRGSG
                     LVGTPDERVAVMVNEEDHLRLQVIRPGLDIREAWEVMNRLDDEIGGRVRYAFSPRLGY
                     LTACPSNVGTGLRASVMVHVPGLVLMNEINPVIKGVTKIGLAVRGLWGEGTEAVGNMF
                     QVSNQITLGEKEGEIVHGLEQIVLEIVEHEKNARARLRDRKEPVLRDHVGRSLGILQN
                     AHILASKEALDLVSGLRLGLEMGILKCKDRRPLDELLVVTQPAHLQKREGRKLKPRNR
                     DVARAQQVRQVLAESGLKLA"
     gene            complement(12392..12910)
                     /locus_tag="PK634_04840"
     CDS             complement(12392..12910)
                     /locus_tag="PK634_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOW97096.1"
                     /translation="MLCESCQQREATVHLTQVVDDSVKKVHLCEECAAQKGLDVHGPV
                     SISDLLMGLGKAVEPQKEKPSVDERSCPRCHMRTADFKKTGRLGCPDCYEAFAVELGP
                     LIKGMHRSEQHVGKTPGGVPARPPPAPARDDLAALQRALEKAVAAEQYEEAARLRDRI
                     QAARAAAGEKAP"
     gene            complement(12927..13796)
                     /gene="ilvE"
                     /locus_tag="PK634_04845"
     CDS             complement(12927..13796)
                     /gene="ilvE"
                     /locus_tag="PK634_04845"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOW97097.1"
                     /translation="MKIYLDGRLVDESKAMVSVFDHGLLYGDGVFEGIRAYNGRVFKL
                     DDHLDRLYRSAKAIALVIPMTKEALARAVVMTCKANGVRDGYIRLVVTRGVGTLGLNP
                     FTCKKPQVIIIAGGIQLYPKKLYETGMSIITVGTMRNQAEAVNPRIKSLNYLNNVLAK
                     IEAMNAGVMECIMLNPQGYVAEASGDNVFALRGKTLLTPPPWCGSLEGITRAVVMELA
                     PEHGLTVNENVMTRYDLYTADEVFLTGTAAEIISVTNVDQRVIGNGKPGPMTRKLAKH
                     FHAVAQRSGTPIE"
     gene            complement(13842..14540)
                     /locus_tag="PK634_04850"
     CDS             complement(13842..14540)
                     /locus_tag="PK634_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW97098.1"
                     /translation="MSNPAASGLFLGAQGLHKTYRVGATEIPVLRGVSLDVRAGESVA
                     VTGASGAGKSTLLHLLGGLDRPDRGTVRLEMKNLYDLSENARAEIRATRIGFVFQSYY
                     LLPELDVLENVMLPAMSRFGAARAAGRLRRRAAELLDRVGLAARAPHRPAELSGGEQQ
                     RAALARALMNDPDLILADEPTGNLDSETGDQVLAHLFGLTRERGRTLVLVTHNDAVAG
                     RCDRRLRLKDGCLA"
     gene            complement(14533..15759)
                     /locus_tag="PK634_04855"
     CDS             complement(14533..15759)
                     /locus_tag="PK634_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW97099.1"
                     /translation="MALPFSLYLALKYLKPKRTFLSVVTVISVLGVLLGVAVLIIVLS
                     VMSGFDEMWREKILSFNAHITVGGWEVIENEDELVERLRKIEGVSGAAPFVQGLVFVQ
                     HNDRVYTPMARGIDPEQERTVSRIPDHMVDGEFNVEDDNVVIGSDLAGQLGVVPGDRL
                     LVYSPQNFTASDEIYLPEELTVAGIFELGMWEFDVGFLLASLPRARDLYGLEQGVHGI
                     QVMTGDPYHADVVAARIEEELGPGTRVTTWMEQNHQLFAALRVEKNMMFFLLIFITVV
                     AAFGITNTLITITVQKTHEIGLLKALGFPSGSIMRVFIWQGWIDGLIGTVAGIGAGLL
                     VLQYRNVLMGWLNRRFGLELLPKELYHLSEIPAVISWTDIGLVALAVLVICTLAGLIP
                     AWRAAKLDPVRALRYE"
     gene            complement(15796..17238)
                     /gene="lysS"
                     /locus_tag="PK634_04860"
     CDS             complement(15796..17238)
                     /gene="lysS"
                     /locus_tag="PK634_04860"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOW97100.1"
                     /translation="MDQGPRENEFRQQRIENMCRLQSLGYEPFGTPFERTGRLAEILA
                     AFAEQKPAKIAGRIVSKRQMGKSIFAHLQDGAARLQVYVKKDAVGEKAFEAFELLDLG
                     DQIGAEGELFVTRTGEQSLKVNAWTLLSKALLPLPEKWHGLHDVEMRYRYRYLDLIAN
                     PEVRSLFDRRTQAIAAIRKFLSDRGFSEVETPMIQAMAGGATANPFKTHYHALGHDMF
                     LRIAPELYLKRLLVGGYDKVFELNRNFRNEGLDRSHNPEFTMLEIYEAYTDVRGMMRL
                     VQDLVTHVAQTVFGALKVGSVERPYDLALPWPEKTYRELILEKAGADWFDLDLPRARE
                     RAQGMGLAIAPEWAKLDVTHEIYEKLVEKSLAGPVFVTRFPAELIPLAKPCADDPTTV
                     DVFELVMQGREIAPAYSELNDPLEQRRRLEAQAKGDESKVDHDFLTALEHGMPPAGGM
                     GIGIDRLLMVLTGAEAIRDVILFPQLKPKT"
     gene            17357..19255
                     /locus_tag="PK634_04865"
     CDS             17357..19255
                     /locus_tag="PK634_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOW97101.1"
                     /translation="MLTIANMSKAFGPGVLFEDAALQVGPGDRIGLVGPNGSGKSTLF
                     SLILGHDAPDAGEVTLDRGLTVGHLPQETAPAGDETVLQLATAVSPEVVELQRRIKEF
                     EAGRHTESHDFHEVQARFDALGGYRLEPRAKTILHGLAFRERDFDRPLREMSGGWIMR
                     AHLARLLVQQPDLLMLDEPTNHLDLETLQWFQGYLKSYPGAILLISHDRDFLDQVVGS
                     IVEIRARKLVRYRGHYEEYLRQRDARDEQQLAAFKTQQRKIKQLQEFADRFRAKNTKA
                     SQAQSKLKQIERMDKIEAPEADRRAIKFKFPQPARSGHRVIALKDIHHAYGENMVYRG
                     VNFEAERGQRLVLVGPNGAGKSTLLKLLAGVLPVQSGARELGLHVKPGYYAQHRVEML
                     QPDRTVLAEALDTPRALTEEFARTVLGSFLFTGDDVFKQVAVLSGGEKSRLALVKLLL
                     DPPNLLLMDEPTTHLDLSSVEALVRALEPYEGTLIFISHDVYFIRRLARQVLHVDAGR
                     LTLYHGDYQYYLDKTAARAVAAAGTAKPAAPSEEKARKRQEAEARQARSREKRKLEKM
                     VDELEAEIARLEARQAELTRELEDPATYENPGRAVAVNRESQDLVEELARVTKEWEDA
                     AERLEALA"
     gene            complement(19294..21462)
                     /locus_tag="PK634_04870"
     CDS             complement(19294..21462)
                     /locus_tag="PK634_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOW97102.1"
                     /translation="MLTKRTAPAVFLALLLAVVRSALAGYEDFVNFPVTGGTYSNGTF
                     LGRDGSTWSYALCRGDRVITAPSPCMNKETTAANITSGSIAGGCGTLTFQWRMGFSAT
                     NSNGTNVCFDVTVNGALVSAVRLGVPGNTYTSSPVAVNQAGSFVLKFAQHDATAKQVI
                     IDNIAWSPYGGGGTHPPELFLTPPATSVVLSATNLVQVDVTATEPDADELRLWATNLP
                     AGALFAGATGAAPLACTFSWTPTLAQTGHVAVVFHAGDKDGTNRQTLAILVLSNKPYY
                     YGTEGQAGPELRAALHEIISTGSRQLTDDQENTAMKDLDTDPADTNNIICLYRRVPIA
                     KSLYNDPNGWNKEHAWPESRGLGGDGPDQNDVHNLFAADVKVNALRGNLYFDESNPAD
                     PSYRNPTTNSAPQTSMDSDSWEPPDEVKGDLARALFYMDVRYDGLEANTEDIQLQDAS
                     SDPSEGRMGVLTTLRAWHEADPPSLAESNRNEKIFQVYQYNRNPFIDHPEWVASIFDP
                     ETTPPVLQPIGVQTGGVGAWLRFEARATATQNDPVTLTISNAPAGAGLYATNTLGTFE
                     WMPDAAGTWTVRVYAADNDGADSEAVPMVVLVDQDGDGLPSWWEQLHFDGGTNATPSA
                     DSDGDLFSNLQEWIAGSNPRDSNSFLAAEVTWQGGALRVSSEDVNTGRVYTLEQAASP
                     ADGAAWDSLRSTNRLEGGEVLFDLAPTQGGASYRIRAEKP"
     gene            complement(21529..21960)
                     /locus_tag="PK634_04875"
     CDS             complement(21529..21960)
                     /locus_tag="PK634_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOW97103.1"
                     /translation="MKKLLKFLFITSDDPNIGLLVLRVFVGAAMLTHGIPKITSGPEF
                     WEKVGAVMGSIGVPGPAVAWGFMAAVAESVGAALLLLGALTTIASFLLVFTMSIAAFV
                     AHAADPFDVKEKALLFLFAALLFLLKGAGRWSVDRLIRRSA"
     gene            complement(22016..23017)
                     /locus_tag="PK634_04880"
     CDS             complement(22016..23017)
                     /locus_tag="PK634_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOW97104.1"
                     /translation="MSVSRPMLVGMLHVPVNTILASPPAHCDALGLPAAAPRDWELLE
                     KLDPLPPFPGLALSEDDMGAGSQRVRALSFFPFIEDRMLAEAAVYVRHGFPALMLENV
                     AGPYFVRGEQPPVIFWVMQALAERLRVAYPEARIGLQVLAFSDDWAMDIACRCGLDFI
                     RCESVLFEGVRPEGRTPNRGNLAKLYIARQQFLARQGSKSAGPQVYADLQKKHTVFPA
                     ELEPLEPWLDNILFQKLEGVIVTGKATGSEVAEEDLRWTREAIDKVKAYTAKTLGRAW
                     SPPLLVGSGVTIENLPLCARYADGLIVGSSLKRGGYWECPLDEERVKSFAGVWNRPL"
     gene            complement(23244..23951)
                     /locus_tag="PK634_04885"
     CDS             complement(23244..23951)
                     /locus_tag="PK634_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW97105.1"
                     /translation="MKRVKILVAEDDAAIARGLEDALESEGYAAVVARDGRAALDRFA
                     REAFDLVLLDVMMPGVNGYDVCREIRKVNADMPVVMLTAKGEEIDKVVGLELGADDYI
                     TKPFGVRELLARIAAVLRRARRQGPPPEPTAELPDVLRFGEVEVDRKKFTAARGKKVH
                     ALTAREVKLVEYFHARPGEVLGRDAILNAVWGLDYLGTTRTLDQHVAQLRKKIEKDPA
                     RPAIILTVHGAGYRYNA"
     gene            complement(23948..25573)
                     /locus_tag="PK634_04890"
     CDS             complement(23948..25573)
                     /locus_tag="PK634_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOW97106.1"
                     /translation="MKRTAVIIYWLILLVSALAVGAGAFRLLLREHSRLESAGLAAAR
                     GGAQLLADEVALSVQELEQKLGENLAAIPSANRAVTLAEWEQGHPLIRNSFVLDARGA
                     VVYPAPTEPMTGEQERFLRRYEALFSGRAAWTGPAAEQPAAPASARQEFSRLAVAQKA
                     LSSSADSAGVPAVQTGWLPWYADNQIQLLGWALDPADGSRYGVELEMAAVLSRLAPAF
                     AGAGEPYALLDGDGRVVFQSGALASNAVPVVAVPVGAVLPHWQVGAYGTGTATAAARG
                     TFLLLTGLQVGVFVLAIILGGSLLLWQAHRHWRDAARKTSFVSNVSHELKTPLTSIRM
                     YAEMLAEGRVKDDVKRADYLKVIVDQSQRLTRLVNNVLDFSRLEQRRRAYRPEPFDLA
                     GAVGETVESQRTRLQEAGLRVEIAPPAAPCPVVADRDAVTQAVLNILDNAAKYAAGGG
                     ELRVEFGADEQGCRARFLDRGPGIPSAHRARLFEQFYRVDDSLSAKQPGCGLGLSIAR
                     RMMRDLGGDVRFEPREGGGAVFELAVPRERAGS"
     gene            complement(25585..26079)
                     /locus_tag="PK634_04895"
     CDS             complement(25585..26079)
                     /locus_tag="PK634_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97107.1"
                     /translation="MKKTILSAIVILVGLLSGGVRAGGPVTVASVDLNRIQNDVGNQR
                     LMWLDMGEEARAEILNLRRSQDRLMVQCLKEDDDSKQAVLQTQLQSINNKLNMLRSAL
                     GNRGNDPRKALTRYIARRYGETYALVYDAQVERNNWSLIVWSASSVPDLTEEIIQTLD
                     RELP"
     gene            complement(26167..27507)
                     /locus_tag="PK634_04900"
     CDS             complement(26167..27507)
                     /locus_tag="PK634_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOW97108.1"
                     /translation="MKSKIILWAAGLALAAASVAGAARTEPRDVLCRIELDRGVLPAD
                     APQRAVVKVTLDAPTLERDGDRPPVNLAIVLDRSGSMSGEKIEKAKEAAIEALRRLGP
                     RDVFSLVIYDDRVETVIPAQEARNAEWMESRIREIGPGGNTALFAGVSQGAAEVRRNL
                     EGRYVHRIILLSDGLANVGPSSPDDLGRLGASLLKEDISVTTVGVGTDYNEDLMTRLS
                     QNSDGNTYFVENYRDLPRIFAAELGDVLSVVARRVVLKIHCLNGVRPVRIIGRDGRVT
                     RDGVEIQLNQLYGGQQKYALVEVEVPAGAEDQVLEIAAADCRYRSALTDRDRTSSSRV
                     EARFSRREDDVRRSANVAVQEEYIRNEIAVAKDKAVEQWDAGERQQAVEDLRKKSEEL
                     KELGATYDVSVEVLTETEALSGEASELDAVGMDKSDRKRYRTDSYQTRNQQMQQ"
     gene            27711..29039
                     /locus_tag="PK634_04905"
     CDS             27711..29039
                     /locus_tag="PK634_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97109.1"
                     /translation="MKRGLVILVLLAALGLFWGAGRLNRPLREERARQHLNPSDPVGD
                     APPLLTFTTVALGGFRGILADLLWLRAARLQEEGQYFELVQLATWVTRLEPRFPQAWA
                     FHAWNMAYNISVLFAEPANRWRWVRHGITLLRDEGLKYNPGEARLYRELGWLFQHKIG
                     AAMDQTHLFYKEAWAREMAALFDGPAPDYERLRPEVRRRMANEYRLDPDLMRKVEEQF
                     GPLDWRLPQAHAVYWAWRGRPFAAGFELQACNRMIYQSLRDAFLQGRLAYDTDEGLLL
                     MLPNLDVLPGLLRTLEEFIAMPAWSAEMRDIRRSILGDAALMYYTLNREEEARRYFEE
                     WKAGVPSEETPPDFESFVFKNYVGEIEGSAPPDYGVLIEQTLYQSLYWQALGYADRAA
                     GYERLAALCRDRAGSGFPPLDEFRRSVRQRLLGELRSEQARARIRDEAAW"
     gene            29101..29532
                     /locus_tag="PK634_04910"
     CDS             29101..29532
                     /locus_tag="PK634_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97110.1"
                     /translation="MRNILGVVCILFGALGWIGQLISAVNYALAQRLGLQEKSEGTDP
                     LFRTAERNAARWDSLVLWTLILAGVLMLLDSRWWPPVALVAGGIYLDGAGREVAKVWS
                     LKSQGIRIGTPGAQRVQKVFFTMMAAIALGVLACAFRRLAG"
     gene            complement(29542..30816)
                     /locus_tag="PK634_04915"
     CDS             complement(29542..30816)
                     /locus_tag="PK634_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97111.1"
                     /translation="MAWPRTRFQLLLAGVTAWSRFPAAPGALARLQARRLRALLDYAF
                     RHSACYRDAMTARRLRPDDFRAPEDLGKLPVLEKSAVRERAADLLSDEFSFADCPRRS
                     THGTTGEPLGIPTLPLERWLDSVLWARVYPDAGFRPWQRQAKIALPSRIPTRRYLAQR
                     LGLFRRTHVAATEPVAEKIRALRAARPQALVCWASTLDEITAELERADTVLDIPLVFS
                     TSSMLWPVVRERAGRRLGAKVTDIYGSIETGPIAWECEERHGYHVHSDRVIVELLDEA
                     GRPARAGRVVCTVLWRRAFPLIRYALGDLAEWADGPCPCGRPYPLLKALSGRATDLVE
                     LPDGSRVSSVTLRAALFGTPGIEQFQLVQETPTRFRLRVVARDAFTADIERSVIDEFR
                     RQYAGALELRVERVPDLHVAPGAKFRPVVLLK"
     gene            complement(30894..>31073)
                     /locus_tag="PK634_04920"
     CDS             complement(30894..>31073)
                     /locus_tag="PK634_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97112.1"
                     /translation="WPGATGRTYCVYLSTGLQSSVWSPLPGYTNIAGTAGEMTINVGQ
                     PPDSAPRFYKVRVRH"
BASE COUNT         5099 a        10566 c         9996 g         5412 t
ORIGIN      
        1 gtttcaatcc acgcccccgc gcggggggcg actggccggg ccgccgttgg agctgacgtt
       61 ttcgaccagt ttcaatccac gcccccgcgc ggggggcgac aacccgtgcc ggttgcgcgt
      121 cccgacctcg cggttgtttc aatccacgcc cccgcgcggg gggcgactct gggtgatggc
      181 gataaatggg gatgcccttg tttcaatcca cgcccccgcg cggggggcga ccacacgggc
      241 aacctccttg aattgttttt aagggtttca atccacgccc ccgcgcgggg ggcgacatcg
      301 catccgtggt taacggaaac acctgaaatg tttcaatcca cgcccccgcg cggggggcga
      361 cttgcgccac tccgccattg actgtgtacc agtgcatgtt tcaatccacg cccccgcgcg
      421 gggggcgaca tggccccgcg cttctctgcg atctgaagcg ccgtttcaat ccacgccccc
      481 gcgcgggggg cgacattcgt tccagagcaa tctggtccgg ttgatcgccg tttcaatcca
      541 cgcccccgcg cggggggcga cagacgcaag gggtcacgtt cttgtacgtc taatgtttca
      601 atccacgccc ccgcgcgggg ggcgaccgtc cacattctag acgcgcgaat cgcagggtca
      661 atatgatccc ctaccgcgaa gatccttcga tttggttgtc aaagaaccca tctgccggaa
      721 ggcatctgcc cgtatccgtc tcgctacaaa gcaattacgc cgccccgcga atccccctgc
      781 catttgatgg gtgcttgatc ttcgcggagc cgtcagacga caagcaatcc ttcctccaga
      841 tcggcaattt ttttattccc ataatgctct acacggcggc gccagttgga cccgagatag
      901 tagaaacgca ggctatcctc gtcttccttg catatatcca gcaggcggtt attcagtatt
      961 gcccattggg ccgggtccac attgcactcg aaaacggaat tctgcacgcg ttggccataa
     1021 tcaagacacg ccttcgccat ccttcgcagc cgagccttgc cccgattgtc caccgtcgaa
     1081 acatcatagc tgaccagcac gagcatcgct acctccagag gaaaggcgga tacgcatcca
     1141 ggtcgccgcg aagatgccgg gccatcagaa gcgcctgcac gtggaagagc agtccgaccg
     1201 gtacatcttc ctcaatgaac ggatggtata tctcctcctg tttgcgcttc tgatacgcga
     1261 gcaaaagcgc cttgcgtgtt tcgtcgtcca tgaccacggc tcccgattcg cttttcacga
     1321 agccgccggc ctgcacctgc cgcagattca cgagggacag cgccagccgg tccgcgagaa
     1381 acgggcgaaa ttcctctatc atgtcgaggg ccagagcggg cctgcccggg cgtagggcgt
     1441 ggagatagcc gacttgcggg tcgagtccca cggtttcgag cgcggcggag gcgtcgtgaa
     1501 caagcagggt gtagagaaaa gacaatagcg cgttgatgtt gtccaacggc gggcgacggc
     1561 tgcgcgcgac gaacttgaaa tcctccttct gggccgtgat cagatgatcg aacaccccga
     1621 aatacaggtt ggcggcctcc ccttcccgcc cgcgaaggtt atccagcgga atgtcgttct
     1681 ccagttcctt gagcagggag cccagccgcg ccactactct ctcgatatcg tccatgccgc
     1741 ccttgccctc atggtcccgg gcggcacgct gtagcacggt tctcgaattg gcgatcttcc
     1801 cgatgacgat ggagctcgcc agagcggacg cggcttccgg ctgatcggcc cgacgatact
     1861 gctctcggcg caggagcacg ttgccggaga ccggcccttc cacccgcgcg tgaaagcggc
     1921 cgcgctccga aaggtgcgtg atcctaacgc cgcgttctcc gcacaagccc atcagcggcg
     1981 cgctgcagga caccaggccg aaacagacaa tcccgccgat cgtgtggata gggactcgaa
     2041 gccggacctc gttttcgacg cgcaccagca ccgattcgcc ctcatggcac agataggcgc
     2101 cctgcgtcgt cacgaaaagc gtattaagca gtttcttcat ggagcatctg tcccaggtaa
     2161 cgcttggcgg acttgccgcg cccagccacc tccggcaggc aggacaattt caacgagcaa
     2221 ttgtcgcatt tcttctcgta gacagcgcgc ggcgtgaggc cggaatcgag aagatcatga
     2281 agccgggccg ccgacgcttc cgtttggtga cgcagttcag cggaaaagac tgcgccatgc
     2341 cgccgacggg gctggccgta gaagatcgca ccttcgggaa tctgcgcgcc gagcatctcc
     2401 tccaggcaaa aggcctgggc gcaaagctgg acttcatcgc aggtgtcggg cttgggccgc
     2461 ccacgcttgt actctacagg aaacggctgc cacgaaccgt cggcctgccg atggaactcg
     2521 acgacatcgg cctggccggt tatgcccagt cgaagcgaac gcaggcggag cccgcgcgcg
     2581 attcggacgt cgccccgggt ttcgtccccg ccctcgtgca ccttttcatg taaaatccgg
     2641 ccttccgctg tcagccgatt ctcatcccag acgccttcca agtgaatgag ggcgcactgg
     2701 cgctcgcaga acatgaggtg ctggagcgca gagagcggca gcaaatcgtc ttccgaatac
     2761 acttatttcc tcccgtagtg cgctcttttt tctctcacac tgaaaccgat ctgtttgcgg
     2821 gttggttccg gcggtgacat caattgacgg attgcttcaa atagcgtctg aatcccttct
     2881 tcattgcttg cgacccgacg ctccaattcg gtcagtttcg cagcgagttc atggtgggcc
     2941 gccagaagcc cgcgcagacg gacaaaggct cgcatgatcg ccacgttcac gtgcactgcg
     3001 cgctcgcttc gaagcacgcc cgaaagcatc gcaactccct cttcggtgaa ggcgtaaggg
     3061 cgataccgcc tgcctccacg gccttcgttt gaggttccaa tctggaacct caaacgcgca
     3121 acttcctctc gcgcaagagg aaacacaaag tcggccggaa accgatcgcg attccgactg
     3181 accgccttgt tcaactcctt tgtcttcaca ccgtacagcg tggcgagatc gctatccagc
     3241 atcacccgct ggccgcggag gaggtagatc atcccctcca cccgcgaagc ggggagtcgc
     3301 tctctagcca tgatccgccc tcctacagca tctcgatcag ctcgacgccg gcaggaactc
     3361 cagccttgtt gaccctgacc ttgtagtcgg agtaggcccg cgcaggcttg gagacatcac
     3421 ccgacctctc gacgccaacc aaatcaaaca gcgcttgcgc gggggccttc ccaagcttgt
     3481 cggcgtgctt gaagacaatc agctttctcg ccgccatctt gccgcgagcg gcggagtggt
     3541 cgtgctcgaa catattctgg agagcctgcc agagcagttc cagatcgtcc tcggagaagc
     3601 ctgtctgggc cgccaagggg gctgagatga agccttcgct ccggtagagc gcgtacggca
     3661 ccgcatgttt gcgccccatc gtgcggttgt ctccagcttg atctttcgcc tcctgctccg
     3721 tctccaccgc catacgcgtg atactcaatt ccaagccggc aatcggttcg acgctccgac
     3781 caaagctgaa ctgtaccggt cctcgaacct gcccgcagtt gttgacctta gttgacatca
     3841 ccgcgccaaa cgtacggacg tcatagaagt gctcgcacat ccaggcgcgt gccgccgcaa
     3901 tacgtttctc ctcgtccccc tccacctcgg tcgccttgta ggctggggct cgcgaggtcg
     3961 agagcacggc cttctccttc acatagatca tgaagggcgc ctcatcgtct ttggccaact
     4021 gtacgtagtt gcgcaccttg cgtttcaggc atacatcggt gacaatcccg tgccccgttt
     4081 ccggatcgat gcgcggcatg ttcccggcgt ccgggtctcc gttggggttt ccattttcaa
     4141 catcgaacag gtagacaaac tcgaatcggt tcttgatggc ttcagtcatg ggttatttct
     4201 cctccttggt tccctgcttc ttggccaggc gttgatggta gtaaccgagc gcgaacatgc
     4261 cctgctgctc aaggttcaat cgcggcggaa aacgctccgg caatcctccc acgatgtcag
     4321 cgttcaggtt ctccatcgtc acggccaatc ccttcttgtc gccagagagc ttggataagt
     4381 ggtgctggtt cagtcgaaca agtactggga ataccgcagc cggcgtcgcg gatgcggacc
     4441 cgtagtaacg atcacggatg gtcgcattga tccccggatt cgcgtattcc tgcgtccttt
     4501 ccagcaccgc aaacaaccga ccgagcagat aaccgggctc ccgacagtcg ggattgagac
     4561 tcatactcac ctccatgttg tagtttctaa ccagcaccgc tttgatcaaa gcagctcgaa
     4621 agtagctcac attcgcttcc ggctttcccc tcttggtctt cgccgcctgt tccgcgtgtg
     4681 cgcgagccag caaggtcggg agaagggacg cgggatagcg accgccggaa agcacggacc
     4741 gcatcatcgc gtctgccagg gtctcgggga tattcttggc ttcatgcagt acggcggttt
     4801 ccggcagaag ccgatcaagc gcgggatact cgggctcatt ctcaaaccga cgcttgatcg
     4861 ccagatcctc gaagtgccgt ttcagcctct cccccatctc gcccaccgtc ccggcgtacc
     4921 agaagcgcac gctcaaccgc gccttggccg gagcggccaa gccgagcatg taaaacggaa
     4981 cggacgggtc gaggtccttc gggaaacgac cggagcgaac ggcctccagc cattcgcgca
     5041 atcgggccaa ttctgcagta tcatccgagg tctcgaacac catccccatg aacgattcag
     5101 ccttggatgc ttgtgccgtc cagtagagcg cggtggcatc cgccaattga atccgctgat
     5161 ggccctgttt gagcaggtgg ttcaacgctg tcgtgtattc gaacgccgcc gactcactca
     5221 ccggagcgtt gaagctctgc tccttgccgt aggagcagac agcatcgaag ttgaacgaca
     5281 ccagcgcggc gcctgtggtc tgagcgttcc ggacgccttt gatagaagga tgcaagcgtg
     5341 caatgggact tgggttgccc gtaacgaggc agacgccgcc agttgagtcc tttgtctgag
     5401 gcatgtgttc ccaagctcgc ttcagtcgct ctctgttgtg cagataacct tcgtccgcgt
     5461 actcgaagac cacggttgtg ccaacccatt ccatccatcg atcgagtgcg gctgccttct
     5521 cggcttccca cgtatcgagg aatcgcaaca tcgcttccgc agccggatca tccacgcctg
     5581 caagaaggtc gcggtggagc tttcgaaaac tgtcgaactt ctcagccgca cgttccgcct
     5641 tgcccttgcc atcatctccg agcgcgtacc ccgtttcgtc tcccagaaac cccggcttga
     5701 tggacgcgcc cgttctgccc gttggtcgcg ccgggatgac cagaagtgcc ggcctcggct
     5761 tgcccttacc ggtgattctg atgtcgcgca cttgcagaat ctggccgtca tgaccgagca
     5821 caagggatgt cgtcaccccc tgcgcacagt agcccggcgg cgcaatgccc gatgaggaat
     5881 cggaagccaa ccgaccatag taccgtgcaa gcgcctgtag aatcatggct tcacctccgc
     5941 tggtgtgaat ggtggaacat ccagcacacc gtccaccagc ttggcatcga agaagtgcgg
     6001 cctccggtcg ttggcgtggt cgatatcgtg gagcatgaat cccagatcgc gctccccttt
     6061 gacaggcgag gcggggatct ccgcaaccgg ctcgaacaac gccgggaact cacggcaacc
     6121 gagatacggc cggtgatagc acgccccgtc tttcacgcga cggttgaact ggtcgaggtg
     6181 tttgccttcg ttgcgctcgc cggacacgat ctcgaaatgg gcctcaatca cgtactctac
     6241 gtcgcgcagg atcatcgcgg cacgctgctg cctcttgtct tcgacgaaga gcgccaaact
     6301 cccctgtcca cgtcgcatcg cggtcagcat gttgtcgacg gaagctttgt ccgacacctc
     6361 gttccgccgg acgctcgtga agcgaatcgg cttcagcacg tgaattttgt ccaccgccca
     6421 acggatttcc ggcttccagt agatcgcctc caagacaccc cgcgcggccg agggcgtcat
     6481 cacgtcgtag ctgacccgct ccgccttcat ctcaggccgc gtgaagcagg cgtaatcccc
     6541 ccgcactcgc actctcacgc catagctcat cctcgcttct cctttccgtc gaaatagagc
     6601 gcctcgggct cgaacacctc ggacacctcg acattcagac ccgtgcgcgg atcgtacgcg
     6661 tcgtcgcgaa tcagaacgaa atagcgttcg tgaatctgct ccaccgcgct cccgagcttc
     6721 gccaggtcct gcgcgtagac attcacactg aagcgctgga gcttgcggag gagaccgccc
     6781 gacgggccat cgcgacgcaa cgcagcgatc aagcctcgcg cctcctcgtc gtagggcacg
     6841 agcacgggcc aaccgtcatc acgaatcatg cgaaactcgc gggcaatgcc ctcaaacgga
     6901 atccagaggg tcttcagatc gaattcccga gtccgcctca ggattcccgg cacatccatg
     6961 cgatcggatt gcgtccagta atgccgctca aagtaggcac gcacggcatc aaggctcagc
     7021 ggatcatctg cgaagagcgg aagaacgagc tcggcggctt gtgcggcctg ccgcaggaga
     7081 ccggcgggcg gcggcttctc gccctgaaag acgaatgtct ctcctcgatc gagccgtccc
     7141 tcgcgattgc agcgtccggc ggcctgcgcg atcgaatcga gtcccgccat ggcccggaac
     7201 acaaccggga aatcaacatc cacgccggcc tcgatgagtt gcgtgctcac cacccgacaa
     7261 gcagcgtttg gcgtcgccaa acgctccctg atcgtcgcga tgacggcccg acggtgcgtt
     7321 ggacacatca gtgcgcttaa gtggaagcag tcatcgccgg ccagcagtcc atgaagcttc
     7381 cgagcatgcc gacgtgtgtt gacaatgcac aatgcgcgcg gatgtttccg caacgcttca
     7441 cccacttctt catcggtctg cttgcccagc actcgcacat tgacgcgctt cagctcagcc
     7501 gcgagcgcag agggatcggg aatgatctcg tggacatggt caatcccctt gggaaactgc
     7561 gcacaccgat tcagcgccgg ctgggttgcc gtgcaaagaa gaacggtcgc gccatagtcg
     7621 gcaacgagct cccggagagc ctccaggcag ggctccagaa gggcgacagg cagagcctgt
     7681 gcctcatcca ggatgatcac gcttcgcgcc agattgtgca gcttccggca ggcggacact
     7741 cgattgccgt ggatcgactc aaagagctga acattggtcg tcacgaccac cggcgcatcc
     7801 cagttctccg tcgcgagctt cgcgacatcg tcacgttcat ccgggtccag gttactgtgg
     7861 tgttcgatca caccatccgc gcaatcgccc agagccttgc ggaagacatc cgcgttctgc
     7921 tcgatgatgc tggtgaacgg gagagcgtag atgacccggc cgagaccgtg cgtgcgggca
     7981 tggcgcaacg cgaaggccag tgatgacaac gtctttccac cccccgtggg caccgtaagc
     8041 gagaaaaagc tgggcggcca actcgacgcc tgtacgcagt catgcaggac gcgacgacgc
     8101 gcatgactaa cggcggccga gcctttggcc ggcagcatct ccaagtaccg gaaaagcctt
     8161 ttctccattc gtccaatgtc tggaagacct cctcgcgcct tcgcttgttc gggtttcatg
     8221 aaggcttccg tgttcaggaa gtccgcatcc acaagacagg agaagagcat gcgaacgaag
     8281 aacgcgatct gatagaggta tcgatccttg tccagccaag agagctgtct gggcagtgtc
     8341 ttcggcgaga gaatgtcggg tggaacgagc gagaagtctt cgacaacctt ccgcaagcgc
     8401 gcttgaagat cggaacttac ggcggaagag ccgttcggca accccccgtg atggccggca
     8461 atcacgtagg cgatcagctt ccctgcctga ggatcgatgt caccggccac ggcatgttgc
     8521 gcacctgcgg tcgagtggtc gacgtgctct gtcgaaccgt tcagccgccg ctggaatgct
     8581 tctgagtatt ttcccaagtc atgccacagc cccgccagat atgcccaatc ctccgccccg
     8641 aatggcttgg caaacttagc ggcgagttcg gcggtgcact tgagatggtc ctccaagaga
     8701 tgccaccttt tcggcggcgc gctttcccgg ctatgggcgt agaatgggcc cggcatgtat
     8761 gaatctcccc ctgtctctcc aaagccgatt ctctcacgcc tgtcaagcgg agggtttcag
     8821 cagggagatg ggatcggcgt caactcgcgc cggcgagctg gccgaacttg agggcgtcgc
     8881 cgtcggcggt gacctcgatg gcctcggagc cggcgaggtg gccgctcaag agctcttcgg
     8941 cgaggggatc ctcgaggtat ttctccacag cgcggcgcat gggccgcgcg cccaggaggg
     9001 gatcgaatcc cttgccgatc aggaacttcc gggccgggtc ggagaggtgc agttcgatat
     9061 tcttcgccgc gaggcgtttc cggatcttgg cgacttcgag gtcgaggatc ttctccatgt
     9121 cctcgatgcc gagctgccgg aagacgatga cttcctcgac gcggttgagc agttccggcc
     9181 ggaagacgcg cttggcctcg tcgagcatct gcctcttcag cgcgtcgtag tccgcggcgg
     9241 cgtcggtgcg gctgaagccc atcgccccgc ccttgcgcac gaactcggag ccgaggttgg
     9301 aggtcatcag cacgaccgtg ttgcggaagt ccacgctgcg cccgaggctg tcggtcagtt
     9361 tgccctcctc gaggatctgc aggaggatgt tcatcgtgtc cgggtgggcc ttctcgatct
     9421 cgtcgaagag gaccacgctg tagggccggc gccggacctt ctccgtgagc tgcccgccct
     9481 cctcgtaccc gacgtagccc ggcggcgagc cgaccatgcg cgagacgttg aacttctcca
     9541 tgtactcgga catgtcgatc tggatcagcg cgtcggggtc gtcgaacatg aactcggcca
     9601 gggccttggc gagcagggtc ttgccgacgc cggtggggcc gagcaggatg aacgagccga
     9661 tcggccggcg cgggtccttg agctcggcgc gggagcggcg cagggccttg gagatcgcgg
     9721 ccacggcggc gtcctgcccg atgacggtct tgcggatgtt cgcttccatc tggaggagct
     9781 tcttggactc gaggtcggcc atcttggtca gcgggacgcc ggtccacttg gatacgatgt
     9841 ggcggatgtc ctcggcatcg accgtgacga tcttgccgtc ctgctccttg cgccacgcct
     9901 ccatggcctt gtcggcctcc tcgcgggcct ggcgctcctg gtcgcggaag gcggcggcct
     9961 gctcgaactg ctggagcttg atcgcctcct ccttctgctt ccggatctcc tcgatcttct
    10021 tctcctgctc cttgaggtcg ggcggccggg tcatcgcgga gatgcggccg cgcgcgccgg
    10081 cctcgtccat gacgtcgatc gccttgtccg gcaggtagcg cgaggtcagg tagcgcgagg
    10141 agaggtggac ggcctcgacg atcgcctcgt cggtgatctt cgcgtggtgg tgctcctcgt
    10201 agcgcgggcg gaggccgcgg aggatctcga cggcctcctc gatcgtcggc tcgcgcacgg
    10261 tgatggactg gaagcggcgc tcgagggcgg cgtccttctc gatgaacttc cggtactcgg
    10321 ccagggtcgt cgcgccgacg cactgcagct cgccgcggga aagcgcgggc ttgatgatgt
    10381 tggacgcgtc catcgcgccc tcggccgagc ccgccccgac gatgctgtgc agctcgtcga
    10441 tgaagaggat gacgttcttg ttccggcgga tctcgtccat gacggccttg atccgctcct
    10501 cgaactggcc gcggtacttc gtgcccgcga ccatcagcgc caggtcaagc gtgaccacgc
    10561 gcttgccgcg cagcagctcc ggcacgtcgc cgcgggtgat ggcctgggcc aggccctcga
    10621 cgatcgcggt cttgccgacg cccgcctcgc cgaggagcac ggggttgttc ttggtccggc
    10681 ggcagaggat ctggatcacg cgctcgatct cgttggcccg gccgatcacg gggtcgagct
    10741 ggcccttgtt cgccaggtcg gtcaggtcgc gcccgaacgt gctcaacgcc ggggtcttgg
    10801 aatccttggc ggcggccggc ttgccctcgg ggccggcggg cccgccggac ggctcgccgg
    10861 gcggcggctc catggacggg tcgtagccgg gatccagctc cttcatgacc tcaacgcggg
    10921 ttttttcgag gtccacctcg aggttcttca gcacgcgggc ggcggcgccc tcgccttcgc
    10981 ggagcaggcc gagcaggagg tgctcggtgc ccacgtaggc gtggttcagg gcacgggcct
    11041 cgctgccggc cagggccagg accttcttca tgcggggggt gaagggcaca ttgccggcgg
    11101 tcttggtcgc cggaccgacg ccgacggcct tctcgacctc caggcgcacg gtctcgaggt
    11161 cgatgcccat cttgcgcagg acgttgacgg ccacgccctg ccccagcgcg accaggccga
    11221 ggagcaggtg ctccgtgccg acgtagccgt ggttgaaccg gtcggcctcc ttgcgggcca
    11281 actggaccac ctgctgcgcc ctgggtgtaa aattgttcat ggccaaccgc ctacgccagc
    11341 ttcaagccgc tttccgccag gacctgccgc acctgctgcg cccgcgccac gtcccggttg
    11401 cggggcttca gcttgcgccc ctcccttttc tggaggtgcg cgggctgcgt gacgacgagc
    11461 agctcgtcca gcggccgccg gtccttgcat ttcaatatac ccatttccag gcccaatcgc
    11521 aaaccggaaa ccaggtccag cgcctccttg gaggccagga tatgcgcgtt ctgcaggatc
    11581 ccgagcgacc gcccgacgtg gtcgcgcagg acgggctctt tccggtcccg cagacgggcc
    11641 cgcgcgttct tctcgtgctc gacgatctcc agcacgatct gctccaggcc gtggacgatc
    11701 tcgccttcct tctcgccgag ggtgatctgg ttggacacct ggaacatgtt gccgacagcc
    11761 tccgtgccct cgccccacag gccgcggacc gccagtccga tcttggtgac gcccttgatc
    11821 acgggattga tttcgttcat gagcacgagg cccgggacgt gcaccatcac gctggcgcgc
    11881 aggccggtgc cgacgttgga cgggcaggcg gtcaggtagc cgaggcgggg cgagaaggcg
    11941 tagcgcaccc ggcccccgat ctcgtcgtcc agccggttca tgacctccca ggcctcgcgg
    12001 atgtcgagtc ccggccggat cacctgcagg cggaggtggt cctcctcgtt gaccatcacg
    12061 gccacgcgct cgtccggcgt gccgaccagc ccgctgccgc ggccccggcc ggcgtgctcg
    12121 tggctgatca ggtgccgctc gaacaggatc tgccggtcca actcgtccag ctcttccatc
    12181 cccacggcgt aggatccgga gagggtcggc gcggcctgca gcgtggggcg cagggactgc
    12241 cagatcttct cgcactcctc ctcgccggcc cagccgggga aggcggcgtt gtgcaggttg
    12301 cgcgccagcc ggatgcggct gctgaccacc gggtcggacc cgcccccggc atccagccag
    12361 gcggccgggc ggccgatgag atcgtccacg ttcacggggc cttctccccg gccgcggcgc
    12421 gcgcggcctg gatccggtcc cgcagccgcg cggcctcctc gtactgctcc gcggcgacgg
    12481 ccttctccag cgccctctgc agcgcggcca ggtcgtcgcg ggcgggggcc ggcggcggcc
    12541 gggcgggaac gccgcccggc gtcttgccga catgctgctc gctgcggtgc atgcccttga
    12601 tgagcggccc cagctcgacg gcaaacgcct cgtagcagtc cgggcagccc agccggccgg
    12661 tcttcttgaa atccgccgtc cgcatgtggc agcgcgggca ggagcgctcg tcgacggacg
    12721 gcttctcctt ctgcggctcg accgccttgc ccaggcccat cagcaggtcg gagatggaga
    12781 ccgggccgtg gacgtcgagc cccttctggg cggcgcactc ctcgcagagg tggaccttct
    12841 tgacggaatc gtccaccacc tgcgtgaggt gcacggtggc ttcccgctgc tggcaggatt
    12901 cgcaaagcac ggccgttcct cccggctcac tcgatcggtg tgccggaccg ctgggcgacc
    12961 gcgtggaaat gcttcgccag cttccgggtc atggggcccg gcttgccgtt gccgatcacg
    13021 cgctggtcca cgttggtcac gctgatgatc tccgccgcgg ttccggtcag gaacacctcg
    13081 tcggcggtgt acaggtcgta gcgcgtcatc acgttctcgt tcacggtcag cccgtgctcg
    13141 ggcgcgagtt ccatcaccac ggcgcgcgtg atgccctcca gcgagccgca ccacggcggc
    13201 ggcgtgagca gcgtcttgcc gcgcagcgcg aacacgttgt cgcccgacgc ctcggccacg
    13261 tagccctgcg ggttgagcat gatgcactcc atcacgccgg cgttcatggc ctcgatcttg
    13321 gcgagcacgt tgttgagata gttcaggctc ttgatgcgcg ggttcacggc ctcggcctgg
    13381 ttgcgcatcg tgcccacggt gatgatggac atccccgtct cgtagagctt cttggggtag
    13441 agctgtatcc cgcccgcgat gatgatcacc tgcggcttct tgcacgtgaa gggattgagc
    13501 ccgagcgtcc ccacgccccg ggtcaccacg agccggatgt agccgtcccg gacgccgttg
    13561 gccttgcacg tcatcaccac ggcgcgggcc agggcttcct tcgtcatcgg gatcaccagg
    13621 gcgatggcct tggcgctgcg gtacaggcgg tcgaggtgat cgtcgagctt gaacacgcgc
    13681 ccgttgtacg cgcggatgcc ctcgaacacg ccgtcgccgt agagcaaccc gtggtcgaac
    13741 accgacacca tcgccttcga ctcgtccacc agcctgccgt ccagatagat cttcatgtcc
    13801 gccgttctcc aatggcgggg aactctagca caacggcggg cctatgccaa acatccatct
    13861 ttcaggcgca ggcggcggtc gcagcgcccg gcgacggcgt cgttgtgggt caccagcacg
    13921 agggtgcggc cgcgctcgcg ggtgagcccg aagaggtggg ccaggacctg gtcccccgtc
    13981 tcggaatcca ggttgcccgt gggctcgtcg gcgaggatca ggtccggatc gttcatcagg
    14041 gcgcgggcga gcgcggcgcg ctgctgctcg ccgccggaga gctccgccgg ccggtgcggc
    14101 gcgcgggcgg ccagcccgac gcggtccagc agctccgcgg cccgccggcg caggcggccg
    14161 gccgcgcgcg cggcgccgaa ccggctcatc gccggcagca tcacgttctc cagcacgtcc
    14221 agctcgggca ggaggtagta ggactggaac acgaagccga tccgcgtcgc gcggatctcg
    14281 gcccgcgcgt tctcggagag gtcgtacagg ttcttcatct ccagccgcac cgtgccccgg
    14341 tcgggccggt ccaggccgcc gagcaggtgc agcagcgtgc tcttccccgc gccgctggcc
    14401 ccggtcaccg cgaccgactc gcccgcccgg acgtcgagcg agacgccgcg caggacgggg
    14461 atctccgtcg cgccgacgcg gtaggtcttg tgcaggccct gcgcgcccag gaacaggccg
    14521 ctggcggcgg ggttactcat agcgcagggc cctcacggga tcgagcttcg cggcgcgcca
    14581 ggcggggatc agcccggcca gcgtgcagat gaccagcacg gccagggcaa cgaggccgat
    14641 gtccgtccac gagatcaccg cggggatctc gctcaagtgg tagagctcct tcggcagcag
    14701 ctccagcccg aaccggcggt tgagccagcc catcagcacg ttgcggtact gcagcaccag
    14761 caggccggcg ccgatgcccg cgacggtgcc gatcaggccg tcgatccagc cctgccagat
    14821 gaacacgcgc atgatgctgc ccgacgggaa cccgagggcc ttgagcaggc cgatctcgtg
    14881 cgtcttctgc acggtgatgg tgatgagcgt gttcgtgatg ccgaacgcgg cgacgacggt
    14941 gatgaagatc agcaggaaga acatcatgtt tttctccacg cggagcgcgg cgaagagctg
    15001 gtggttctgc tccatccagg tggtcacccg cgttccgggg ccgagctctt cctcgatccg
    15061 cgcggcgacg acatcggcat ggtagggatc gccggtcatc acctggatcc cgtggacgcc
    15121 ctgctccagc ccgtacaggt cgcgcgcccg gggcagcgag gcgagcagga acccgacgtc
    15181 gaattcccac atgcccagct cgaagatccc cgcgacggtc agctcctcgg gcaggtaaat
    15241 ctcgtccgag gccgtgaagt tctgcggcga gtagaccagc aggcggtcgc cggggacgac
    15301 gcccagttgc ccggccaggt cgctgccgat gaccacgttg tcgtcctcga cgttgaactc
    15361 gccgtccacc atgtggtcgg ggatccggct gacggtccgc tcctgctcgg ggtcgatgcc
    15421 gcgggccatc ggcgtgtaca cgcggtcgtt gtgctggacg aagacgaggc cctgcacgaa
    15481 cggcgccgcg cccgagacgc cctcgatctt ccgcagccgc tcgaccagct cgtcctcgtt
    15541 ctcgatgacc tcccagccgc cgacggtgat gtgcgcgttg aagctcaata tcttctcgcg
    15601 ccacatctcg tcgaagccgc tcatcacgga caggacgatg atcaggaccg cgacgccgag
    15661 gagcaccccg aggacggaga tcaccgtcac caccgagagg aacgtccgct tcggcttcag
    15721 gtacttcagc gccaggtaga gtgaaaacgg cagggccatc ggattaaccg cggaggccgc
    15781 agaggctcgc agaggtcatg tttttggctt caattgcggg aacaggatca cgtcccggat
    15841 cgcctcggcg ccggtcagga ccatcagcag gcggtcgata ccgatgccca tgccgccggc
    15901 cgggggcatg ccgtgctcca gcgcggtcag gaaatcgtgg tccaccttgg attcgtcgcc
    15961 cttcgcctgg gcctcgagcc ggcggcgctg ctccagcggg tcgttcagct cgctgtaggc
    16021 cggcgcgatc tcgcggccct gcatgaccag ctcgaagacg tccaccgtcg tcgggtcgtc
    16081 ggcgcagggc ttggccagcg ggatcagctc cgccgggaac cgagtgacga agaccggccc
    16141 ggccagcgac ttctcgacga gcttctcgta gatctcgtgc gtcacgtcca gcttcgccca
    16201 ctccggcgcg atggcgaggc ccatcccctg cgcgcgctcg cgcgcccggg gcaggtccag
    16261 gtcgaaccag tccgcgccgg ctttctcgag gatcagctcg cggtacgtct tctcgggcca
    16321 gggcagcgcc aggtcgtacg ggcgctccac gctgccgacc ttcagcgcgc cgaagacggt
    16381 ctgcgcgacg tgcgtgacga ggtcctgcac gaggcgcatc atcccgcgca cgtccgtgta
    16441 ggcctcgtag atttccagca tcgtgaactc ggggttatgc gaccggtcga ggccctcgtt
    16501 gcggaagttg cggttcagct cgaagacctt gtcgtagccg ccgaccagga ggcgcttgag
    16561 gtacagctcc ggcgcgatgc ggaggaacat gtcgtggccg agcgcgtggt aatgggtctt
    16621 gaacgggttc gccgtcgcgc cgccggccat ggcctggatc atcggcgtct cgacctcgct
    16681 gaagccccgg tccgagagga acttccggat cgcggcgatg gcctgcgtcc ggcggtcgaa
    16741 gagcgagcgc acctcggggt tcgcgatcag gtccaggtag cggtagcgat agcgcatctc
    16801 gacatcgtgc aggccgtgcc acttctccgg cagcggcagg agggccttgg agagcagcgt
    16861 ccaggcgttc accttcaggc tctgctcgcc ggtccgcgtg acgaacagtt ccccttcggc
    16921 gccgatctgg tcgccgaggt cgaggagctc gaaggcctcg aaggcctttt cgccgacggc
    16981 gtccttcttg acgtagacct ggaggcgcgc ggcgccgtcc tggaggtggg cgaagatgct
    17041 cttgcccatc tgccgtttcg acacgatgcg gccggcgatc ttcgcgggct tctgctcggc
    17101 gaaggccgcg aggatctcgg ccagccggcc cgtgcgctcg aagggcgtgc cgaacggctc
    17161 gtagccgagg gattgcaacc ggcacatgtt ctcgatgcgc tgctgccgga actcgttttc
    17221 gcgcgggccc tggtccatgg ttcgtctcct gcgacggtcc caaaggatag gagccgcctc
    17281 ccggcaagtc aaggaagggt ccagccccga attcgtcgct gactccggcg ctccgatgtc
    17341 gtaccgttcg cctcccatgt tgacgatcgc aaacatgtcc aaggccttcg gcccgggcgt
    17401 cctgttcgag gacgccgcgc tccaggtcgg accgggggac cggatcgggc tggtcgggcc
    17461 caacgggtcc ggcaagtcca cgctgttctc gctgatcctg ggtcatgacg cgccggacgc
    17521 cggcgaggtg acgctggacc gcgggctcac cgtcgggcac ctgccccagg aaaccgcgcc
    17581 ggccggcgat gaaaccgtgc tgcaactcgc cacggcggtc tcgccggagg tggtggaact
    17641 gcagcggcgg atcaaggagt tcgaggccgg gcgccacacc gagtcgcacg atttccacga
    17701 ggtgcaggcg cgcttcgacg cgctcggcgg ctaccggctc gaaccccggg cgaagactat
    17761 tctccacggc ctggcgttcc gcgaacgcga cttcgaccgg ccgctgcggg agatgagcgg
    17821 cgggtggatc atgcgggcgc acctcgcccg gctgctcgtc cagcagcccg acctgctcat
    17881 gctcgacgag ccgacgaacc acctcgacct cgagaccctc cagtggttcc agggctattt
    17941 gaagagctac cccggcgcga tcctgctgat ctcgcacgac cgcgacttcc tggatcaggt
    18001 cgtcgggagc atcgtcgaga tccgcgcgcg gaagctggtc cgctaccgcg gccactacga
    18061 ggagtacctc cgccagcgcg acgcccggga cgagcagcag ctggctgcgt tcaagacgca
    18121 gcagcgcaaa atcaagcaat tgcaggaatt cgccgaccgg ttccgcgcca agaacaccaa
    18181 ggcttcgcag gcgcagtcca agctgaagca gatcgagcgt atggacaaga tcgaggcgcc
    18241 ggaggccgac cggcgcgcca tcaagttcaa gtttccccag cccgcgcgca gcgggcaccg
    18301 ggtgatcgcg ctgaaggaca tccaccacgc ctacggcgag aacatggtct accgcggcgt
    18361 gaatttcgag gccgagcgcg gacagcggct cgtgctcgtc ggccccaacg gcgcgggcaa
    18421 gtccaccctg ctcaagctcc tcgcgggcgt gctgccggtc cagtccggcg cgcgcgagct
    18481 cgggctccac gtgaaacccg gctactacgc ccagcaccgc gtcgagatgc ttcagcccga
    18541 tcgcaccgtg ctggccgagg cgctcgacac gccgcgggcg ctgaccgagg aattcgcgcg
    18601 gacggtgctg gggtcgttcc tcttcaccgg cgacgacgtg ttcaagcagg tcgccgtctt
    18661 gagcggcggg gagaaaagcc ggctggcgct ggtcaagctg ctgctcgacc cgccgaacct
    18721 gctgctcatg gacgagccga ccacgcacct cgatctctcc agcgtcgagg cgctggtccg
    18781 ggcgctggag ccgtacgagg gcacgctgat cttcatcagc cacgacgtct acttcatccg
    18841 ccgcctcgct cgacaggtcc tgcacgtgga cgccgggcgg ctgacgctgt accacggcga
    18901 ttaccagtac tacctcgaca agacggccgc gcgggcggtc gccgcggccg gaacggcgaa
    18961 gcccgcggcg ccgtcggagg agaaggcacg aaagcggcag gaggccgagg cgcgccaggc
    19021 gcgctcgcgc gagaaacgga agctggaaaa aatggtggac gagctcgaag cggagatcgc
    19081 gcggctggag gcgcgacagg cggaactgac ccgcgaactg gaagacccgg ccacgtatga
    19141 gaatcccggc cgcgccgtcg ccgtgaaccg cgaatcgcag gacctggtcg aagagctcgc
    19201 gcgcgtcacg aaggagtggg aggacgcggc ggagcggctg gaggccttgg cctgaatcgg
    19261 cgtgacctgg ccgtcccggc cgtgcgccga tgatcagggc ttctctgccc ggatgcggta
    19321 actcgcgccg ccctgcgtcg gggccaagtc gaacaggacc tcgccgcctt ccagccggtt
    19381 ggtgctccgg agggaatccc acgccgcgcc gtcggccggc gaggccgcct gctccagcgt
    19441 gtagacccgg ccggtattca catcttcgct ggacacgcgc agcgcgccgc cctgccaggt
    19501 cacctcggcg gccaggaacg agttgctgtc ccggggattg gatccggcga tccattcctg
    19561 gagattgctg aacaggtcgc cgtccgagtc cgccgagggc gtggcattgg tcccgccgtc
    19621 gaagtgcagt tgctcccacc agctcggcag gccgtcgccg tcctggtcca cgaggacgac
    19681 catcggcacg gcctcggaat ccgcgccgtc gttgtcggcg gcgtagaccc gcacggtcca
    19741 ggtccccgcc gcatcaggca tccactcgaa tgtgcccagc gtgttcgtcg cgtacagccc
    19801 cgcgcccgcc ggcgcgttgc tgatcgtcag cgtgaccgga tcgttctgcg tcgcggtcgc
    19861 ccgggcctcg aaccgcagcc aggcccccac cccgccggtc tggacgccga tcggctggag
    19921 caccggcggc gtggtctcgg gatcgaagat gctggccacc cactccgggt gatcgatgaa
    19981 cgggttgcgg ttgtactggt acacttgaaa gattttttcg ttccggttgc tttccgcgag
    20041 gctcggcggg tcggcctcgt gccacgcccg caaggtcgtc agaacaccca tccgcccctc
    20101 ggatggatcg ctcgaagcgt cttgcagttg gatatcctcg gtgttcgcct ccagcccgtc
    20161 gtaccggaca tccatgtaga agagcgcgcg ggcaaggtcg cccttgacct cgtcgggcgg
    20221 ctcccacgag tcgctgtcca tgctggtctg cggagccgag ttggtcgtgg gattccggta
    20281 ggagggatcg gccgggttgc tctcgtcgaa atagaggttg ccgcgcaggg cgttgacctt
    20341 gacgtcggcg gcgaagaggt tgtgtacgtc gttctggtcc ggcccgtcgc cgcccagacc
    20401 cctcgattcc ggccacgcat gctccttgtt ccacccgttg ggatcattat agaggctttt
    20461 ggcgatcggg acccggcgat agaggcagat gatgttgttc gtgtcggccg ggtcggtatc
    20521 caggtccttc atcgcggtgt tttcctggtc gtcggtaagc tgccgcgagc cggtcgagat
    20581 gatttcgtgg agggcggcgc gcagttccgg gccggcctgg ccttcggtcc cgtagtagta
    20641 cggcttgttc gagagcacca ggatggcgag cgtctgccga ttggttccgt ccttgtcccc
    20701 cgcgtggaaa accacggcga cgtgaccggt ttgggccagg gtcggcgtcc aggagaacgt
    20761 gcaggccagg ggcgccgccc cggtcgcccc ggcaaacaag gcgcccgccg gcaggttcgt
    20821 cgcccacagg cgcagttcgt cggcatccgg ctccgtcgcg gtgacgtcca cctgcacaag
    20881 gttggtcgcg gacagcacca cgctggtcgc cgggggggtc aggaatagtt ccgggggatg
    20941 cgtcccgccg cccccatacg gagaccaagc gatattatcg atgatgacct gtttcgccgt
    21001 cgcgtcgtgc tgggcgaatt taaggacgaa gctccccgcc tggtttaccg ccaccggact
    21061 gctcgtgtac gtgttgcccg ggacaccgag ccggaccgcg ctgaccagcg cgccgttgac
    21121 ggtcacatcg aaacacacgt tggtcccatt ggagttcgtg gcgctgaagc ccatgcgcca
    21181 ctggaaagtc agcgtgccgc agccacccgc gatcgagccc gaggtgatgt tcgcggccgt
    21241 ggtttccttg ttcatgcagg gcgaaggcgc cgtgatcacc cgatcgccac ggcacagggc
    21301 ataggaccac gtcgagccgt cacggcccag gaaggtcccg ttggagtagg tcccgccggt
    21361 cacgggaaaa ttgacgaaat cctcgtaccc ggccaacgcc gagcgcacga cggccagcag
    21421 cagggccagg aaaacggccg gggcggttcg cttcgtgagc atgccgcttt atacccgctt
    21481 tcgcaggaac tgttcacgat cgaaatggcg gcccggctgg ccgcgcgctc acgcggaccg
    21541 ccggatcagc cggtcgaccg accagcggcc cgcgcccttg agcaggaaca ggagcgcggc
    21601 aaacaggaag agcagcgcct tttccttgac gtcgaacggg tcagccgcat gggccacgaa
    21661 ggccgcgatg ctcatggtga agaccagcag gaatgaggcg atcgtcgtga gcgcgccgag
    21721 caggagcagc gcggcgccca cgctctcggc cacggcggcc atgaagcccc acgccaccgc
    21781 cggaccgggc accccgatgg accccatcac tgcgcccact ttctcccaga attccggacc
    21841 gcttgtgatc ttcggaatac cgtgcgtcag catcgccgcg ccgacgaata cccgcagaac
    21901 gagcagcccg atattggggt cgtccgaggt gataaacagg aacttgagca gtttcttcat
    21961 gaccgcctcc catttttccg aaacgtaccg cagggaaccc gtttcgcaag ggaagctaca
    22021 gcgggcggtt ccacacccct gcaaacgact tcacccgctc ctcgtccaac gggcattccc
    22081 agtagccgcc ccgcttcagc gaggagccca cgatcagccc gtccgcgtac cgcgcgcaca
    22141 gcggcaggtt ctcgatcgtc acgcccgacc cgaccagcag cggcggcgac caggcgcggc
    22201 ccagcgtctt cgcggtgtag gccttcacct tgtcgatggc ctcgcgggtc caccgcaggt
    22261 cctcctcggc cacttcgctc cccgtggcct tgcccgtgac gatcacgccc tcgagtttct
    22321 ggaaaaggat gttgtccagc cacggctcga gcggttccag ttcggccggg aacaccgtgt
    22381 gcttcttctg caggtccgcg tacacctgcg gcccggcgct tttgctgccc tgtcgcgcca
    22441 agaattgctg ccgcgcgatg tacagcttcg ccaggttgcc ccggttcggc gtccggccct
    22501 cgggccgcac gccttcgaag agaacgctct cgcaccggat aaaatccagg ccgcaccggc
    22561 acgcgatatc catcgcccag tcatcggaga acgccaggac ctggagcccg atccgcgcct
    22621 ccggatacgc gacccgcagc cgctcggcca gcgcctgcat gacccagaag atcaccgggg
    22681 gctgctcccc ccggacaaaa tacgggccgg ccacgttctc cagcatcagc gccgggaagc
    22741 catgccgaac gtataccgcc gcctcggcca gcatccggtc ctcgatgaac ggaaaaaacg
    22801 aaagggcccg gacgcgctgc gacccggcgc ccatgtcgtc ttccgacagc gccagtccgg
    22861 ggaacggggg cagcggatcg agtttctcca ggagttccca gtcccgcggc gcggcggcgg
    22921 gcaggcccag cgcgtcgcag tgcgcgggcg gcgaggccag gatcgtgttg acgggcacat
    22981 gcagcatccc caccagcatc ggtcgtgaca cgctcatgcc ctgactgtcg ccgaacgatc
    23041 ccgcccctgt caagaaagcg aggctgtgaa ccacgggctc gtgggaactc gcccctccaa
    23101 actccctgca agcaagattt ggagggcgga gctccagcga cgccgccggg tcaaggagac
    23161 gggatgaagg aacaccacgg atttcacgga ttacacggac aagggacagg cgattatccg
    23221 tgtaattcgt gattctttct ggattacgcg ttgtaccggt accccgcgcc gtggacggtc
    23281 aggatgatcg ccgggcgggc gggatctttt tcgattttct tgcgcaactg cgcgacatgc
    23341 tggtccagcg tgcgcgtcgt gccgaggtag tcgaggcccc agacggcgtt gaggatggcg
    23401 tcgcggccga gcacctcgcc cggacgggcg tgaaaatatt cgaccagctt cacctcgcgg
    23461 gccgtgagcg cgtggacctt tttcccgcgg gccgcggtga atttcttccg gtccacctcg
    23521 acctcgccga agcgcagcac gtccggcagt tcggccgtcg gctccggggg cggtccctgc
    23581 cgccgcgccc ggcgcaggac ggccgcgatc cgggccagca gttcgcgcac gccgaagggc
    23641 ttggtgatgt aatcgtccgc gcccagctcc aacccgacga ccttgtcgat ctcctcgccc
    23701 ttggccgtga gcatgaccac gggcatgtcg gcgttgactt tgcggatctc ccggcagacg
    23761 tcgtagccgt tgacgccggg catcatgacg tcgaggagca ccaggtcaaa cgcctcgcgc
    23821 gcgaaccggt ccagcgcggc ccggccgtcg cgcgcgacga ccgccgcgta accctcgctc
    23881 tccagcgcgt cctcgagccc gcgcgcgatc gcggcgtcgt cctcggccac caggatcttc
    23941 acccgtttca tgaccccgcc ctttcccgcg gaactgcgag ttcgaagacg gcgcccccgc
    24001 cctcccgcgg ttcaaaccgc acgtcgccgc ccaggtcgcg catcatccgg cgggcgatgc
    24061 tcaagcccag cccgcaaccg ggttgcttcg ccgagagcga atcatccacc cggtagaact
    24121 gctcgaagag ccgcgcccgg tgcgcggacg ggatgcccgg accgcggtcc aggaaccgcg
    24181 cgcggcagcc ctgttcgtcc gcgccgaact cgacccgcag ttccccgccc ccggccgcgt
    24241 acttggctgc gttgtcgagg atgttcagca cggcctgcgt caccgcgtcg cggtcggcga
    24301 ccacgggaca cggcgcggcc ggaggcgcga tctcgacccg caggccggcc tcctgcaggc
    24361 gcgtccgctg cgactcgacg gtctcgccga ccgccccggc caggtcgaac ggctccggcc
    24421 ggtacgcgcg gcggcgctgc tccagccggc tgaaatcgag gacattgttc acgagccggg
    24481 tcagccgctg gctctggtcc acgatcacct tgagatagtc cgcccgcttc acgtcatcct
    24541 tcacgcggcc ttcggccagc atctcggcgt acatgcggat cgaggtcagc ggcgtcttga
    24601 gctcgtgcga gacgttcgag acgaacgacg ttttccgggc ggcgtcgcgc cagtgccggt
    24661 gcgcctgcca cagcagcagc gacccgccga ggatgatcgc cagcacgaac accccgacct
    24721 ggaggccggt cagcagcaga aacgtccccc gcgccgcggc cgttgcggtg ccggtcccgt
    24781 aggcgccgac ctgccagtga ggcaggaccg cgcccaccgg cacggccacg accgggaccg
    24841 cgttcgaggc gagggctccc gactggaaca cgacgcgccc gtcgccgtcc agcagggcgt
    24901 acggctcccc cgccccggcg aacgcgggcg ccaggcgcga aagcaccgcc gccatttcca
    24961 gttccacgcc gtaccgcgat ccgtccgcgg gatcgagcgc ccagcccagc agttgaatct
    25021 ggttgtcggc gtaccagggg agccagccgg tctgcacggc aggaacgccg gcgctgtccg
    25081 cggacgacga caaggccttc tgcgccacgg ccaggcggct gaattcctgg cgcgcggaag
    25141 caggggccgc cggctgctcc gccgccggcc cggtccaggc cgcgcggccg gagaagagcg
    25201 cttcgtaccg gcggaggaac cgctcctgct cgccggtcat cggctcggtc ggcgccgggt
    25261 agacgaccgc gccccgcgca tcgaggacga aggagttccg gatcaacgga tggccttgct
    25321 cccactcggc cagcgtcacc gcgcggttcg cggacggaat cgcggccagg ttctcgccca
    25381 gcttctgctc caactcctga acggacaagg ccacttcatc cgcgaggagt tgcgccccgc
    25441 cccgcgccgc ggccagcccg gcggactcca gccggctgtg ctcgcggagg agcaggcgga
    25501 acgcgcccgc ccccaccgcc agcgcggaca ccagcaagat cagccagtag ataatgaccg
    25561 cggtgcgttt catcccggcc ccgattatgg cagttcccgg tccagggttt gaatgatttc
    25621 ttcggtcagg tccggcacgc tggacgcgct ccacacgatc agggaccagt tatttctctc
    25681 cacctgggcg tcgtaaacca gggcatacgt ttcgccatac cggcgggcga tataccgggt
    25741 gagggccttc ctcggatcgt tgccccgatt ccccagcgca ctgcgcaaca tgttgagctt
    25801 gttgttgatg gactggagtt gcgtctgaag gacggcctgc ttcgaatcgt catcctcctt
    25861 caggcattgg accatgagcc ggtcctgcga ccggcgcagg ttcaggattt ccgccctcgc
    25921 ctcctcgccc atgtcgagcc acatcagccg ttgattaccc acgtcatttt gaatccggtt
    25981 caggtccacg gacgccacgg tgacaggtcc ccccgcccgg acgccgcccg acagcaagcc
    26041 gaccagtatt acgatcgccg atagaatggt ctttttcatg aaggttcctc gatgcaatga
    26101 aatgccgctt cgacgcatgc cgccgcgcga cgcccgggtg gggcgccgcg cggacgggcc
    26161 cgtgcgctat tgctgcatct gctggttccg ggtctggtag ctgtcggtgc ggtaccgctt
    26221 ccggtcgctc ttgtccatgc ccactgcgtc gagctcactc gcctcgccgg acaacgcctc
    26281 ggtttccgtc aggacttcga cactgacgtc gtacgtggcg cccagttcct tgagctcctc
    26341 gctcttcttg cgcaggtctt ccacagcttg ctgccgctcg ccggcgtccc actgctcgac
    26401 ggccttgtcc ttggccacgg cgatctcgtt gcggatgtac tcctcctgca cggcgacgtt
    26461 ggccgagcgg cgcacgtcgt cctcccgccg gctgaaccgg gcctcgacgc gggagctgct
    26521 ggtgcggtcg cgatcggtca gggcgctgcg atagcggcag tccgccgcag cgatctcgag
    26581 gacctggtcc tcggcgccgg cgggcacctc gacctcgacg agggcgtact tctgctggcc
    26641 gccgtagagc tggttgagct ggatctccac gccgtcgcgc gtgacgcgcc cgtcgcgccc
    26701 gatgatgcgc acggggcgga cgccgttgag gcagtggatc ttcagcacca cgcggcgcgc
    26761 gaccacgctc aacacgtcgc ccagttccgc cgcgaagatc cgcggcaggt cgcggtagtt
    26821 ctcgacgaag tacgtgttgc cgtcgctgtt ctgcgacagg cgggtcatca ggtcctcgtt
    26881 gtagtcggtc ccgacgccga cggtcgtcac ggagatgtcc tccttgagca gcgacgcccc
    26941 gagccggccc aggtcgtccg gcgaactggg gccgacgttg gcgagtccgt cggagagcag
    27001 gatgatccgg tggacgtagc gcccctcaag gttccgccgg acctccgccg cgccctggct
    27061 gacgccggcg aagagggccg tgttgccgcc cggcccgatc tcgcggatgc gcgattccat
    27121 ccactccgcg ttccgggcct cctgggccgg gataacggtc tcgacgcggt cgtcgtagat
    27181 gaccagcgag aagacgtcgc gcgggccgag ccggcgcaag gcctcgatgg cggcctcctt
    27241 ggccttctcg attttctcgc cggacatcga gcccgaccgg tccagcacga tagccaggtt
    27301 cacgggcggc cggtccccgt cgcgttccag cgtgggggcg tccagcgtga ccttcaccac
    27361 ggcgcgctgg ggcgcgtcgg cgggcaggac cccgcggtcc agttcgatac ggcacagcac
    27421 gtcgcgcggc tccgtccggg cggcgcccgc gaccgacgcg gccgccaacg ccaggccggc
    27481 cgcccacagt atgatcttcg acttcatggc ttttctccca ttgttggttg ttctatccgg
    27541 tccggtcaag agcgcgcgct gccccaacct ttccgcacac cattggaccc ccaccacgcg
    27601 gaaatgtcgt cacgggcctg caaaacgttt gtaaaagaaa tgtcatgccc ggcggcccgc
    27661 cagcgccctc ttgtgcgggg gccgggccgg ggcttatagt ggcgccgggc atgaaacggg
    27721 gcctcgtcat cctggtgctg ctggctgcgc tcgggctgtt ctggggcgcg ggccgcctga
    27781 accgaccgct gcgcgaggag cgggcgcggc agcacctgaa cccctccgat cccgtcggcg
    27841 acgcgccgcc gctgctgacc ttcaccacgg tcgcgctcgg cggattccgc gggatcctcg
    27901 ccgacctgct gtggctgcgc gccgctcggc tgcaggagga gggccagtac ttcgagctgg
    27961 tccaactcgc cacgtgggtc acgcggctgg agccccggtt cccgcaggca tgggcgttcc
    28021 acgcctggaa catggcctac aacatcagcg tgctgtttgc cgagcccgcc aaccgctggc
    28081 gctgggtgcg tcacggcatc accctcctgc gcgacgaggg cctgaagtac aatcccggcg
    28141 aagcgcggct ctaccgcgag ctgggctggc tgttccagca caagatcggc gccgcgatgg
    28201 accagaccca cctcttctac aaggaggcct gggcccgcga gatggccgcg ctgttcgacg
    28261 ggccggcccc ggattacgag cgcctccggc ccgaggtccg ccggcggatg gcgaacgagt
    28321 accgcctcga cccggacctg atgcggaagg tggaagagca gttcggcccg ctggactggc
    28381 gcctgcccca ggcccacgcc gtctactggg cctggcgcgg ccggccgttt gccgcgggct
    28441 tcgagttgca ggcctgcaac cgcatgatct accagtccct gcgcgacgcc ttcctgcagg
    28501 gccggctggc gtacgacacg gacgaaggat tactcctgat gttgcccaac ctcgacgtgc
    28561 tgcccggcct gctgcgcacg ctcgaagagt tcatcgccat gcccgcgtgg agcgcggaga
    28621 tgcgcgacat ccgccgctcc atcctcgggg acgccgcgct gatgtactac acgctgaacc
    28681 gcgaggagga agcccgccgc tatttcgaag agtggaaggc cggtgtcccg tccgaggaga
    28741 cgccgccgga ctttgagtcc ttcgtcttca agaattatgt cggcgaaatc gagggttcgg
    28801 ccccgccgga ctacggcgtg ttgatcgaac agaccctgta ccaaagcctg tactggcagg
    28861 cgctcggtta cgcggatcgc gccgcgggct acgaacgcct ggcggcgctg tgccgggacc
    28921 gggccgggag cggattccct ccgctggacg agttccggcg aagcgtccgg cagcggctgc
    28981 tgggcgagtt gagaagcgaa caggccaggg cgcggatccg ggacgaggcg gcctggtagg
    29041 gcgcgttggc caacgcgccg ccgcctccgg cggcctctac cggacgggaa ggagagtgcc
    29101 atgagaaata tactgggtgt cgtctgcatc ctgttcggcg ccctgggctg gatcgggcag
    29161 cttatttcgg cggtgaacta cgcgttggcc caacgactcg gcctccagga aaaaagcgag
    29221 ggcaccgatc ccctgttccg gacggcggag cgcaacgctg cccggtggga cagcctggtg
    29281 ctctggaccc tgatcctcgc cggcgtcctg atgctgctgg acagccggtg gtggccgccg
    29341 gtcgcgctcg tcgcgggcgg gatctacctc gatggcgcgg gccgggaggt cgccaaggtc
    29401 tggagcctga aatcacaggg catccggatc ggcacgcccg gcgcgcagcg ggtgcagaag
    29461 gtttttttca cgatgatggc cgcgatcgcg ctcggggtgc tggcctgcgc cttccggcgc
    29521 ctggccggct gacgggcccg gttatttcag cagcaccacc ggcctgaact tggcgccggg
    29581 cgcgacatgc aggtccggca cgcgctcgac gcgcaactcg agcgcgccgg cgtactggcg
    29641 gcgaaattcg tcgatcaccg agcgctcgat gtccgcggtg aaggcatcgc gcgccaccac
    29701 gcgcaggcgg aaccgcgtcg gcgtttcctg cacaagctgg aactgctcaa tgccgggcgt
    29761 tccaaacagc gccgccctca aggtcacact gctcacgcgc gagccgtccg gcagttccac
    29821 gaggtcggtg gcccggcccg acagggcctt gagcaacggg tacggccggc cgcacgggca
    29881 gggtccgtcg gcccattcgg ccaggtcgcc gagcgcgtag cggatcagcg ggaaggcccg
    29941 ccgccacagc acggtgcaga cgacgcgccc tgcgcgggcg ggacggccgg cctcgtccag
    30001 cagttccacg atcactcggt cgctgtgcac atggtagccg tggcgctcct cgcattccca
    30061 ggcgatgggg cctgtctcaa tcgagccgta gatgtccgtc accttcgccc ccaggcgtcg
    30121 gccggcccgc tcccgaacga ccggccagag catgctcgat gtgctgaaca cgagggggat
    30181 atcgaggacg gtgtccgcgc gctcgagttc ggccgtgatc tcgtccagcg tgctggccca
    30241 gcagaccagg gcctgcggcc gcgcggcgcg cagcgcccgg attttttcgg cgaccggctc
    30301 cgtggccgcc acgtgggtcc gccggaacag cccaaggcgc tgggccaggt agcgccgggt
    30361 cgggatgcgc gacggcagcg cgatcttcgc ctgccgctgc caggggcgga agccggcgtc
    30421 cgggtagacg cgagcccaca ggacggaatc cagccagcgt tccagcggca gcgtcgggat
    30481 gcccaggggt tcgcccgtgg ttccgtgcgt gctccgccgg gggcagtccg cgaaggagaa
    30541 ttcatcgctc aacaggtcgg cggcccgctc gcgcacggcg gatttttcca ggacggggag
    30601 cttccccagg tcttccggcg cccggaaatc gtccgggcga agtcgccggg cggtcatggc
    30661 atcgcggtag caggccgaat gccggaaagc atagtccaac aacgcgcgca ggcggcgggc
    30721 ctggagccgg gccagcgcgc ccggcgcggc ggggaaccgc gaccaggccg tcacgccggc
    30781 cagaagcagc tgaaagcgtg tgcggggcca ggccatgcgc aggagctcca gacaaacccc
    30841 accctagcac ccgcgccgcc cgcaagcaaa ccgacgctgc gtcccgccac cgctcaatgc
    30901 cgcacgcgga ccttgtaaaa ccgtggcgcg ctgtccggcg gctggccgac gttgatggtc
    30961 atctcgcccg ccgtgcccgc gatgttcgtg tagcccggca agggactcca cacgctgctc
    31021 tgcaggccgg tggacaggta gacgcaatag gtgcggcccg tcgcgcccgg cca
//
LOCUS       DAOOTK010000026        30991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1064_length_30991_cov_6.040180, whole genome shotgun sequence.
ACCESSION   DAOOTK010000026 DAOOTK010000000
VERSION     DAOOTK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 30991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30991
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_30991_cov_6.040180"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3276)
                     /locus_tag="PK634_04925"
     CDS             complement(<1..3276)
                     /locus_tag="PK634_04925"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOW97113.1"
                     /translation="MKRKNPGELRAGCGFRLIPLLAAIALISAAGAQRAHADLEVAKS
                     IRGGLTRVNAGTQFTYVLSYRAASTTTNFYNAFLTDVLPVGLNYASAVGTPHTSNITY
                     TAATRTIRVNFINPLPAGSTGEIEINVLYPNGTTPDGTITTNRATAQATNSPPYTSNP
                     ITIMATASNRATLTKTLVGSSVPLNQNVSYTVTLNNNISVGALNITNITMTDTLPPGS
                     IFVAASGGGSYDAGSHSVTWTQQTLGIGNMARTVTVRFPAPPFALSNRVTNVVTAIGT
                     PLGKTPTNWIRGITNIIVNPTTASTFTKSVSEARYVYEGKVVNKTFNMTVNNTGNTPI
                     TNVVVTDEIKPEFKVTRFNTGNFNGTPAGLRTGINVYYQKWTGSGWLDAPGNPYTGLT
                     NTTILVSALGLATNDYITDLRWEFGELPVNYSIAGLSFQADAMTTDRAGNPVVAGDVL
                     TNRADLAYYSYLGPSNRSSTVTLNVLSARPVVQLTKAVTNAVPVKDGDTLTFRLILAN
                     RAEAAAALQNPVVADLLAGYLTYVPGSWWIQSGPAGLPEPTFTSAPNYNGTSNTLLRW
                     DWSSYSLPTNASIEIRFRASLTPGTLYGGKTNQIHLVEWDNPALDTNATLYARDVNDL
                     NANGSTNDYVYYQNLGYTISSVAAMDSYKWVKGELDTNWHRYPESGFTVPGGIADYRM
                     TVKNVGNVPVKNINVIDILPFVGDTGVIDLSARDTEWRPSLAAPITAPPGVTVYYSEE
                     GDPARPDFVPSGPPGSDAPYWSTNPPADFTRTRSVKFDFGAAVLQPNQELEFGWPMRA
                     PVGAPTGGEIAWNSFGYYGTRTDNNTQLLPSEPIKVGIKILPDTNAVYGDYVWFDENL
                     DGIQDNSEGGMNGIRVHLWQDSGPGGIPDGVRDTDTDHYIGFTITGDDYEGHPGYYLF
                     PNMDRGFYYALFEVPAGAVVSPADQGGDDTKDSDVTLMAGSNYWTEITWLDDAEHDLS
                     WDMGLWVDEYEITLVKLAGNAPDGTTLWVESGAPVRYTYQVLNNGNLDLVNIRVRDDK
                     LGLIGIIPRLASGQSAVLTSTPVNVTADVTNVGDVRGRPAVPGGPEIPGLPEATDDDD
                     AVVR"
     gene            complement(3620..5695)
                     /locus_tag="PK634_04930"
     CDS             complement(3620..5695)
                     /locus_tag="PK634_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97114.1"
                     /translation="MLAGPAARAQQASNLAAFHRSGQTFLTWDEAGAGDTAESYRVYR
                     HTGEISTGTLASATLLASVPRGSSYYPAESAFRGSNVYFVIENLGPNLASNRGLFVYT
                     PHDSGAFFYAVTLRTNGRENTNEFGAGNALTAAVSEAEADPEPVLVRTSATGRVRTYT
                     QYMDYHRWNPTLGGYAYNYSVCLPANFSNTVAWPLHLKLVSHGSRYDETETGSWYNRG
                     IQIYGDDPDDTWHYGFHENWDYSKSTHPTSGIIVNYTEQRHLRAIHDVIRDPYYRVDT
                     NRIYAWYSSMGGSGAMTLGFHYPDVFAAVFCWQPVTVYRTADFWRGQDFETLWGATNY
                     NLPIENRGPQAGPITNFNGMGVFDWMDHLAMLTHHPGRDVPMFFFHAGKNDGNIEYET
                     QGEPAIGAFYQARCGFVALIDPSSHSGIAFADWPTASTTNWDIRQLAFRKDISFPAFS
                     RASGNDAAPPPAEPLTNFYNKTLEWSCPWNHFAGDIVDTATNYEIVLRSRAGAQHAAV
                     TVRRPQLFKPAPGQGVVWENVPRGESAPAAMGDLRVDAHGLVTVERFVITTNGNRLRL
                     RSDALADSDSDGLPDWWELARGLNPSSTVDAAGVDTDLDGLVNGDEKRTGTAPLSSND
                     FFRIVAAGEPGPTWSSTPGLVYTVQQTTNYSAWEPVSTGIVAMETRTAIPILFPTNHS
                     GPAYRVFTE"
     gene            5834..8242
                     /locus_tag="PK634_04935"
     CDS             5834..8242
                     /locus_tag="PK634_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOW97115.1"
                     /translation="MRSAYSQVGCLVLLALVSCAPPARAGTVLLENFSYADPAEAHKA
                     WLPKDASPPVTAVPGGVTFPCPFDGGADRVYWDRSVALNLDRYTSLELDLTCDHPEAL
                     RSLTLYFKSGDGWFVWGKPLKRAGRQTLLLLKEEFSTEGRPAGWHRIEAIRVSPWRGS
                     AVAASLTLHALTAAEDSLLIVRATASAQDASEEALSARIARRLSSWLRELGLSHGMVS
                     DDDVAAGILRRARVAILPYNPRPTSSMLAALRTFVQNGGRLVVFYGSDPNLAALMEIK
                     LGRYARTDLPGRWSSMTFVDPSARYLPARVFQESSNILPVTPAGKGGKVIAWWEDALG
                     RRTRDPAWVATSRGLWMTHVLQNDDVRTKQEMLLGLLGRLDPALWQEAARHRLNTAGQ
                     VDSFTSLADAREGIGRLGAASGQEVQPLLERAAALHDQMRALYDEQRFLEVIQAGRSL
                     RQVLTEAYARAQPPRAGEFRGVWEHEGVGWFPGDWSRSCRLLSGAGFTAVFPNLLWGG
                     LAHYPGRVLPESYTSRKLGDQAKSCVEAAHRNGLEAHAWMVCWSLQNAPDDFQLRMKK
                     EGRLQVTATGQTIPWLNPADPRNVDLALAAIRELVTRYPVDGVHLDYVRYPGPDSCFS
                     PVSRREFEKDRGRRVDGWPRAAAPGGAVAAEYQRWRAGRITAFVRAVNREIKTIRPTA
                     KVSAAVYSSYPDCIASVGQDWGAWLRDGLLDFVCPMTYTTDAAGFAALTQKHLALPGA
                     AGRLMPGLGVTADESQLASDQAIEQIVAARRLGAPGFMLFDLSHTLREEILPMLRLGL
                     TSPP"
     gene            complement(9039..9263)
                     /locus_tag="PK634_04940"
     CDS             complement(9039..9263)
                     /locus_tag="PK634_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97116.1"
                     /translation="MDAQFMIGYTTAFDRYNDFQGARTDNETVTPGTIRQALQVALDN
                     APPGMEPPTPIIQRIAQVSAQLQTTPASSP"
     gene            complement(9749..12199)
                     /locus_tag="PK634_04945"
     CDS             complement(9749..12199)
                     /locus_tag="PK634_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97117.1"
                     /translation="MPFLINPAVDVSGQFTEAWEHRLASDGSIGLAVWADNGSDEIKG
                     MRLDAQGRPLDTVALLIAPNSVGAYATERRYPAVMWFQDAFWVIWQEHRGSGPREGLG
                     RRMYPDGRFADTTPFPVETCATSAYRSCVCAGGTNLLMTWSAYSNTYRLWAQTCSPAG
                     QPVGAATQVMAGFSGYVQPGLAYGNFAAGEKSFLLAVAISNDIRGLRLSEGGAVLDAA
                     PLPICVFPGTQANPCPAFGPGPGGTQGWWVAWQDSRSGTVDIHGAFVAQDGTVSHSST
                     GMAFITAASDQVAPSLAWDGSRYLLAWHDYRSPPAFRASVMDSGGGFADTNGTLVSTN
                     DPSTPVVCARTNGSFLAAHGDETVWGQAIEAQDSLLTAGTQRLASVAGAGERYNSGGC
                     DGTNFWVAFEYHPAHSERLGDIAIQRVSPGGIPLAAAPFLVCTNSSDQWAPAAAVGRD
                     TVYVVWTDERNPRDQVWGQRLTLDGQPAGTNVQIGVGATFAYFCDIASDGTNFLAVWD
                     DSDSGKTRGRFVRADGSMGGAFDIGGSDISDPFVTFGGGCYLVLCADDAAIRFRRYRP
                     GGTAIDTSASNLYSGSSVRWEPQAAYHAGGNRFLVTWRDQAAYQILGRTLDASTAALG
                     NVITVLVINAWASSANSAVACDGTDWLCLFRDTRNGPAGLFGARVDAGGALLDAAPFP
                     ILRNATFGELVPPAAPLTNVLILGTRPVYDTPGRVYNTGKSLGLFWQPGAQESPGPDV
                     EGRHVRIDAFGQTAFFFRQSAPASTGYVVETCASLANPDWRPVTNGVLLDSGMGVSAL
                     WREPGGAASSGTYRARGN"
     gene            12444..14462
                     /locus_tag="PK634_04950"
     CDS             12444..14462
                     /locus_tag="PK634_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97118.1"
                     /translation="MSTRDRSPLALALTLAILCAGSASAQAPAVTRWMDPQGRLRPAH
                     APEAAGGPLKMTLARVPSARGAPIPPGAETNRVCLVVHTNVVGALGAALSRYQADLGA
                     AGYSVLLYTYESGSAENLRAYLAGLYAQPNSLAGAVLVGEIPYIVYEMYQDWGNGNEY
                     EDFPCDIFYADLNGTWADTNSAPPFSAGKYDSRSGNLDLEIWISRLKAGNLPALGAET
                     DSLARYFDKNHRYRERQLVPHRRALAYVDDDWSYGTGEDAECLGWRYGDAGVRAVMDA
                     DSTTAADYMAFMTNSYELMFVRSHGYPGGHGFYESGKTAFNYVLTDDYLDLAPPALFY
                     SFFVCSGCDFATSDNLGSLVAFNADDSGLACWGSTKTGGMWDDRYFYEPLAEGASLGS
                     AFKAWFNVCQTNWPSYAPPWWYGMVILGDGALALSGTEPAGSAASGWSAAPVVSSPSV
                     QGGRYQLRRTASLAAPAWTNVGSIVTAAQFAVSLSDTNALVDFAAYRVVKLEDGTSNL
                     LRNASFEMPGSSDSRARYWEDANPDSHGNIWSNVSRVAWRCYYGGAEAAVRGTWAGVN
                     IGGWWQEAPATAGSNYQASAWFWADHSANVWTAAVQELKIEFLDSGYSLLGSSSVGLS
                     GVVESWTQKTVSAVAPANTAWARFVVNVSGASAQGALQFDDAELVRSP"
     gene            complement(14463..17786)
                     /locus_tag="PK634_04955"
     CDS             complement(14463..17786)
                     /locus_tag="PK634_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW97119.1"
                     /translation="MSYRAAWLLLAFLCSAPRAPAQSSRPGWGATPYAGGVTFRVWAP
                     HATNLTVAGEFNGWSTTSRPLTLESATSGVWSADVSGAVTGQNYKFLVNTPTTYKTDP
                     RSRRVNAGDNNNSVITAPSNFTWQGDAYSLTNSRDLVLYEAHAETFNGPSGTFSTFTG
                     RLDHLRNLGVSGIELMPVNEFPSSTSWGYNPGYPFSVEYDYGTPDALRELVRQCHQGG
                     LAVLLDVVHNHWDSGSSLWRFDGWDPDGVHGGQYFYNTDPYATTPWGPRPDYSNDQVC
                     AAIFDTFRMWKNEYRMDGFRWDAPNHILYTTNGVYIPEGLTLITNALAMMAAEYPGTW
                     NIAEDVKELSGFNAYWDFTFLWEIRSVLTQGSDSNRDMPTVARNVAGTFQRVIFTDSH
                     DSAGDLNGGVRLPTAIHSGDPAGYYARKRSTLGAALVLTAPGTPMILQGQEMLETNQF
                     SDTVGADWTRTNSFAGIVRLYRDLIRLRRNLDGVSEGLLGDSCSAYHVDNASKLVAYS
                     RWDSAATDRYTVVAANFANATRSGYPINFPAAGTWYVHFNSDSTNYAADYGNAGSVEV
                     VAAGAPPWGTITIAPYSVLVLSQVPRTGMLIRETAPADRPAGNDDGILDPGETIREDV
                     VLWNKSQLAATNVSVALTSPTPGVTIVQGTSSFEDMAPDAAATNASAYEYRLDPGLAC
                     GSLVQFELAMSFNGQALTSAFEHVIGRFVDLATTTNVFESTDTPKPIADASTTYSDLT
                     ISEPGTNVVSDVNVRLRIHHTYDRDLTLALQHPDGSEVLLVYRRGGSGDHFGTGTCGS
                     AAYTVLDQAAALAISNGSPPFAGAYRPESTLDAFNGKALNGTWRLRMRDAFSRNTGTN
                     LCWSLEIAYEQQGCECAAFSNRPPVAAATQVVHVGYGPTNLALTGTDPDGQSLTFKTA
                     GSPAHGSLSAVDTSTWTAVYLPVHGYTGTDSFDFVASDGLTSSAPAALRVAVQAPPDS
                     DADGMPDDWETAQFTNATAGSATADADGDGMPNLDEYRCNTDPNDSNSVLRLVGQPWA
                     AQGYALRWSSVGGTRYGVEYSSGITGDFQHIARSLAEEMDPAPKGAATNRSFTDDFTL
                     TPPPAAGAPRLYRVRVRDE"
     gene            complement(17920..19251)
                     /locus_tag="PK634_04960"
     CDS             complement(17920..19251)
                     /locus_tag="PK634_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOW97120.1"
                     /translation="MKRSLKGLAVFWGRLSPTRLVAIGYLAYAVAGWIVLCLPVVQAK
                     SVTWLDNLFTSVSALSTTGLVTVSTSGSYNLLGQAIILLLIQVGGIGYMTFGSFVVLS
                     RTGALPERRREIARTVFSVPADLHIDHLLRSVIVFTAIIEAAGAAALYGAFRHAGVQA
                     AAWSSIFHSISAFCTAGFSLYDNSLEAYRSDFWINTILGIVSYLGAVGFIVCLDVWRR
                     VTGRARRITLTSRIILISTFWLTVAGMLLFFICEPSIRALDTDDRLLASLFQVMAALT
                     TVGFNSIPISQLSQASLFLMILLMLVGASPSGTGGGLKTTTFTAILGVMRSAMKGAER
                     VYFWGKEIPAARVWLAMASLGFYAGSLILGSFLLALSESHGFENLLFEAASALGTVGL
                     STGITSQLTHVGKLIVIALMFVGRIGPLAFGLSLFCGGKEPRPSGQEDLAV"
     regulatory      19689..19820
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(20567..20905)
                     /locus_tag="PK634_04965"
     CDS             complement(20567..20905)
                     /locus_tag="PK634_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97121.1"
                     /translation="MNKPDGLARWLGTCGVLEIATGLALLATPAAVAYGLLRISPGGS
                     ATGRLAGAALPAYNLTACAALAWAGPARGNGGVPARTAAALLGGLGLALAIALFGRRR
                     ISAETPTLER"
     gene            complement(21079..23865)
                     /locus_tag="PK634_04970"
     CDS             complement(21079..23865)
                     /locus_tag="PK634_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97122.1"
                     /translation="MKTPSPTVLLWSAAVIGALRGNTPVRAEDATWTGMASANWGDTN
                     NWSPRKLPTASDHALIPAGTPSCTNTSGATVDRLTVGGELTLRNGTFNLTTTGDVAGV
                     LNLHGALLRGAGSLTVDGSFNWTANDIDGLGRIVLSEGSVNVISGAASPNKHLICPVE
                     NRGRLIQRNTGTGSFYIYAGGVVDNWGSYEIEDDSYIAWFGTRGEIRNHGVFQKTGGS
                     GRNDIQVHFRNEDGGLIGSTSSTGLSFSASFSAAGTGSIGTGTVNVTAGSGTLGGVLG
                     GPGALRLAGGSLSVTGASEVGCMTLAGGTLMGADVLTVTQSLAWTAGVITGAFSVVLA
                     PGTTNTISGTAVPNKHLRAVLENRGTVRQFGTADTAFFIYAGGVLDNWGSYEIEDDSC
                     IVRDTSQGEIRNHGVFRKAGGSGRSEIRVYFRNEDGGLIGSTSSTGLAFGASFSAAGT
                     GAISTGTVSVTAGNGSMGGALGGPGVLRLAGGFLSVTGASQIGCFALAGGTLTGAEVL
                     TVTQSLAWTAGVMTGACRMVLSPGTTNTISGTATPNKHLGAALENHGRLRQFDTGSGS
                     FYVNRGGVLDNRGIYQIEDDSDITFNVARGVVTNAGWFLKTDGAGISQVQPPFVNATG
                     GVLASRSGCLMLSVLGMQDGILDAGTGATIQVSGGFTNNGVMRGAGSISLGTATVLNR
                     GTAAPGSSVGRLTITGHFAQASSGVLEVELGGTTPGSGHDQLVVTGNAAVDGTLRVTL
                     DGGYFPAEGSIFAFMVHGARSGSFTTSDLPTLPAGSYWGQVQNANQSYLFREPAFTTG
                     GVATAWYRLKGAGPAAGEAWDQAQWDAIDGLDTDLDGDINSREYVADTNPTNGASFFP
                     RVAFAGDFGFTADMAIPQTSTGRMYAVEATVDLRAGDWALILPEEQGVGGTILFILTN
                     NAPFRSYRTRVRLP"
     gene            complement(24231..24314)
                     /locus_tag="PK634_04975"
     tRNA            complement(24231..24314)
                     /locus_tag="PK634_04975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24278..24280),aa:Tyr,seq:gta)
     gene            complement(24363..26564)
                     /gene="glgB"
                     /locus_tag="PK634_04980"
     CDS             complement(24363..26564)
                     /gene="glgB"
                     /locus_tag="PK634_04980"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOW97123.1"
                     /translation="MKPILHEAAILSVVHGTHGSPYDVLGMHATGAEMPGAVARSYQP
                     YAQRVELLEKESGAAHEMARIHNDGLFEVFFPDRAPFAYRLRMTGYDGHQWELEDPYR
                     FPLQITDFDLYLFGEGTHFRTYEKMGAHPMTIDGVEGVHFAVWAPNAIRVSVIGWFNR
                     WDGRHHPMQHRGSSGLWEIFLPALQPGDLYKFEIKGHHGFLAQKADPYAFFSELRPRS
                     ASVVWNIHRHSWKDAEWMERRGRANWHESPISVYELHPGSWRRVPEEGNRMLTYREMA
                     DQLIPYVKGLGYTHIEMLPISEHPFDGSWGYQTIGYYAPTSRFGNPDDFMYFVDRCHQ
                     EGIGVLLDWVPAHFPKDGHGLAYFDGSHLYEHADPRKGEHKDWGTLIFNYGRNEVRTF
                     LLANALYWADVYHIDGLRVDAVASMLYLDYSRKEGEWIPNQFGGRENLEAVDFLKKFN
                     EIIHAEFPGFLTFAEESTAWPMVSRPTYLGGLGFDFKWNMGWMHDMLEYITKESVHRK
                     YHHNDITFSLLYAFTENFILPFSHDEVVHGKRAMLDKMPGDLWQKFANLRLLYAYMHA
                     HPGKKLLFMGGEFGQWREWNHADSLDWHLCQYDSHHKLQTLVGTLNKLYRDFGALHQV
                     DFSWEGFEWIDFHDWEQSIVSFFRRGKDSRDILVCIFNFTPVPRQGYRVGVPLPGVYE
                     EVLNTDADCFGGSNVVNVHTLNTDEIPWMGRPWSVPLTLPPLGAIWLRLIG"
     gene            26820..27209
                     /locus_tag="PK634_04985"
     CDS             26820..27209
                     /locus_tag="PK634_04985"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOW97124.1"
                     /translation="MARRRKSFNRGAWAVQRERFRLEQAAPPADFAVESVADVLPGVV
                     KKLGLAAPFWMESLRRDWPSIVGPDVAKRSRPGHLENKTLTIFVIHPTWLMELKRMER
                     QVLEIIRQSCGREQVRAVRLALDPDAR"
     gene            27281..27354
                     /locus_tag="PK634_04990"
     tRNA            27281..27354
                     /locus_tag="PK634_04990"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27315..27317,aa:Arg,seq:cct)
     gene            27378..28292
                     /locus_tag="PK634_04995"
     CDS             27378..28292
                     /locus_tag="PK634_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOW97125.1"
                     /translation="MSTAPILSGGAAREAAIRAGVITGGIDTVITLGAMVAANSAVLL
                     ADFFKTFLEFLAVLLSWMAIRRINRGGGATYEYGLGKLENLGSLLVGVLMTVCLLIIT
                     GNAVRNMLHPSHIRGLGVWISMGSQVLYSVINGRLGLRGWRMAKAEASPLMASQARLL
                     LTRAVANVFILASLVLSVALSRFHWAQYIDPLSSLVIAASILMAALGIFSSSVSDLLD
                     RTLEESDQLVILRELARYFDEYEALHGIRSRRSGTDVYIDILLEFNAERKMGEIHEVA
                     EQMRRRIEEQIRGSRVTIGLARTGTPAP"
     gene            complement(28333..28665)
                     /locus_tag="PK634_05000"
     CDS             complement(28333..28665)
                     /locus_tag="PK634_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97126.1"
                     /translation="MKKVLLVALIALAAAPVLLQAQTAPAETPADPAAALAQRVDQLS
                     QKLAEQVQGLQKAIKEIQERLGDSYSRPTAFNSIERRLEDIEKRLDKLARDFDDLEDQ
                     VHRLERGR"
     gene            complement(28734..>30991)
                     /locus_tag="PK634_05005"
     CDS             complement(28734..>30991)
                     /locus_tag="PK634_05005"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOW97127.1"
                     /translation="ALVCGYTEASIGGAEFLGVRDLCLTRFDSFGYPEWTRLWGSEET
                     DEACGVAVAGDGTAWVAGTTEGSFGGQTNQGGADICLTRFDGGSYSGALSTVWGETLY
                     TGFQTGTVRVLLLSYLHGTEVDRAERGAPGAFQFDALPNDTWYELFAFRDVNNNDIWN
                     YGEPVGRYERNPFRPATNMTNVIIRLEEPDSDLDGLSDPYEETGTGTDPDNPDTDGDG
                     LSDGYEVGTGSNPLNPASHAVHDGPVREWTVIRPSTNTAYGLQANGVAVDADNRPVLV
                     GDSHESVEGAPALGSGDILLNAYSASRSSLWVRLHGGTSHDFGQRLARVAGGGFAAAG
                     TTYSAFGGQTNSGAGDLCLVRVNATGTVQWVRIWGSAAFDAGADVAADSSGNFYVAGT
                     TDGAWDGQTNAGNSDLCLSKFDAGGTRQWTRIWGSSADERAGGVGADGSGNVYVAGYT
                     DGSFDGQLNGGIVAVCVTKFDAAGQKLWTRIRGPETAAYAMDIAVSGDGLVYAAGYLY
                     GGADGQPAIGSDDLLAMAFDSAGEWRWTRVWGSPAQDTGYGAALDNAGNLYVVGRTHG
                     DFGGQTNASYMFDACVSKFGPDGTRWWDRIWGSASWDQASDVAPDALGNVYVCGFTAG
                     AFDGQTNNGGTATFLTRFGPGAAQDTDGDGLTDTGEQATGTDPSDFGSSLHMMAQAAQ
                     APASANGWIVRWASVSQRVYRLDRTTNLLTGAWTAVATNIPAVVPVNIYTDTTAVGVN
                     PRFYRAHGSLP"
BASE COUNT         4945 a        10145 c        10613 g         5288 t
ORIGIN      
        1 acggaccacg gcgtcatcgt catccgtagc ctcgggcaga ccggggatct ccggcccgcc
       61 gggcaccgcc ggccggccgc gcacgtcgcc gacattggtc acgtcggccg tgacgttcac
      121 gggcgtgctg gtcaggaccg cgctctggcc gctggcgagc cgcgggatga tgccgatcag
      181 gccgagcttg tcgtcgcgga cccggatgtt cacgagatca aggttgccgt tgttcagcac
      241 ctggtaggtg taccgcacgg gcgcgccgct ctcgacccac agggtcgtgc cgtcgggcgc
      301 gttgccggcc agcttgacaa gcgtgatctc gtactcgtcg acccacaggc ccatgtccca
      361 cgacaggtcg tgctcggcgt cgtccagcca ggtgatctcg gtccagtaat tcgagccggc
      421 catcagggtg acgtcggaat ccttcgtgtc gtcgccgccc tggtcggcgg gggagaccac
      481 cgcgccggcc gggacctcga agagggcgta gtagaagccg cggtccatgt tcgggaagag
      541 atagtagccg ggatggccct cgtaatcgtc gccggtgatc gtgaagccga tgtaatgatc
      601 cgtgtccgtg tcgcgcacgc cgtccgggat gccgccgggc ccgctgtcct gccacaggtg
      661 cacgcggatg ccgttcatgc cgccttcgct gttgtcctgg atgccgtcca ggttctcgtc
      721 gaaccagacg taatcgccgt acaccgcgtt cgtgtcgggc aggatcttga tgccgacctt
      781 gatgggctcc gacggcagca gctgggtgtt gttatccgtg cgcgtgccgt agtagccgaa
      841 ggaattccag gcgatctcgc cgccggtcgg cgcgccgacc ggggcgcgca tgggccagcc
      901 gaattccagt tcctggttgg gctggagcac ggccgcgccg aaatcgaatt tcacggaccg
      961 ggtccgggtg aaatccgccg gcggattcgt ggaccagtac ggcgcatccg agccgggcgg
     1021 gccggagggt acgaagtccg gccgcgcggg gtcgccttcc tcgctgtagt agacggtcac
     1081 ccccggcggg gcggtgatcg gggccgcgag gctgggccgc cactcggtgt cgcgcgccga
     1141 gagatcgatg acgccggtgt cgccgacgaa cggcaggatg tcgatcacgt tgatgttctt
     1201 gacgggcacg ttcccgacgt tcttcaccgt catgcggtaa tcggcgatgc cgcccggcac
     1261 ggtgaagccg ctctccgggt accggtgcca gttcgtgtcc agttcgccct tgacccactt
     1321 gtaggagtcc atggcggcga cgctggaaat ggtgtagccg aggttctggt aatagacgta
     1381 atcgttggtc gagccgttgg cgttcaggtc gttgacgtcc cgcgcgtaca gggtcgcgtt
     1441 ggtgtccagc gccgggttgt cccactcgac caggtgaatc tggttggtct tgccgccgta
     1501 cagcgtgccc ggggtgaggc tggcgcggaa ccggatctcg atggacgcgt tggtcggcag
     1561 cgagtaactg ctccagtccc agcgcaggag cgtgttgctc gtgccgttgt aattcggcgc
     1621 ggacgtgaag gtcggctcgg gcaggccggc cgggcccgac tggatccacc agctgcccgg
     1681 cacgtaggtc aggtacccgg ccagcaggtc ggccaccacc gggttctgca gggcggcggc
     1741 ggcctcggcc cggttggcca ggatcaggcg gaaggtcagc gtgtcgccgt ccttgacggg
     1801 cacggcgttg gtgaccgcct tggtcaactg gacgaccggc cgggcgctca acacgttcag
     1861 cgtgaccgtg gacgagcggt tggacggccc gagataactg taataggcca ggtccgcccg
     1921 attcgtcagg acgtcgccgg cgaccaccgg gttgccggcg cggtcggtcg tcatcgcgtc
     1981 cgcttggaag gacagcccgg cgatggagta gttcacgggc agttcgccga actcccagcg
     2041 caggtccgtg atgtaatcgt tcgtggcgag gccgagggcg gacaccagga tcgtggtgtt
     2101 cgtgagcccg gtgtacggat tgcccggcgc gtccagccag ccgctccccg tccacttctg
     2161 gtaatacacg ttgatgcccg tgcgcagacc ggccggcgtg ccgttgaagt tgccggtgtt
     2221 gaaccgggtc accttgaact ccggcttgat ctcgtcggtg accacgacgt tcgtgatcgg
     2281 ggtgttgccc gtgttgttca cggtcatgtt gaaggtcttg ttgaccacct tgccttcgta
     2341 cacgtatcgc gcctcgctga cgctcttggt gaacgtcgag gcggtggtcg ggttgacgat
     2401 gatattcgtg atgccccgga tccagttggt cggggtcttc ccgagcggcg tcccgatggc
     2461 cgtcacgacg ttcgtgaccc ggttgctcaa ggcgaacggc ggcgcgggga agcggaccgt
     2521 caccgtgcgg gccatgttgc cgatgcccag ggtctgctgg gtccacgtga cgctgtgcga
     2581 gccggcgtcg taggagcccc cgccgctggc ggccacgaag atggagccgg ggggcagggt
     2641 gtcggtcatc gtgatgttcg tgatattcag cgcgcccacg ctgatgttgt tgttcagcgt
     2701 cacggtgtag gacacgttct ggttcagggg cacgctggaa ccgaccaggg tcttggtcag
     2761 cgtggcccgg ttgctggccg tcgccatgat cgtgatggga ttggacgtgt agggcggcga
     2821 gttcgtcgcc tgggccgtgg cgcggttggt cgtgatcgtt ccgtccggcg tggtgccgtt
     2881 cggatacagg acgttgatct cgatttcgcc cgtggatccg gcgggcagcg ggttgatgaa
     2941 attcacccgg atcgtccggg tcgccgccgt gtacgtgatg ttcgaggtgt gaggagtgcc
     3001 cacggccgac gcgtagttca gtccgaccgg gagaacatcc gtgaggaaag cgttgtagaa
     3061 gttcgtcgtt gtgctggccg cgcgatagct caatacatag gtaaactgcg ttccggcgtt
     3121 cacccgcgtc aatccgccgc gaatgctctt ggccacctcg agatcggcat gggctcgctg
     3181 cgcgccggcc gcgctgatga gcgcaatcgc ggcaagcagc ggaatcaacc ggaacccgca
     3241 acctgcacgg agttcccccg gattcttcct tttcatgcag acctccccgg cgtcccgagc
     3301 gacacccttc atcattctgc aagcagccaa cattagtaaa tacacgtcac cgccgacccg
     3361 ccccttttcc aagaccgagc aacggttaac aacttctcgc agttctactt aaagaaatta
     3421 gaccatgtct tccgcgttgt ccactttatt agcattggac cttcggccaa tagagtcttc
     3481 cgtcgctgtg ccgataaaaa agcaagagaa atatcgaatc ccatgcttgt gagttgtaat
     3541 ttgttcaaaa tacgaaaatt atgtaatcgg atagttgcgg cattttccgg atctgctcgt
     3601 gtccattcca aatggccggc tactccgtga aaacccggta ggcggggcca gagtgattcg
     3661 ttgggaacag gataggaatc gcggtccttg tttccatggc gacgatgccg gtggacacag
     3721 gttcccaggc tgaataattg gtggtctgct gcaccgtgta gaccagtccg ggcgtgctgg
     3781 accacgtggg tcccggttcg cccgccgcga caatgcggaa gaagtcgttg gaggaaaggg
     3841 gggctgtgcc cgtccgtttt tcgtcgccgt tgaccaggcc gtccaggtcg gtgtccacgc
     3901 ctgccgcgtc cacggtgctg ctgggattga gcccgcgggc gagttcccac cagtccggca
     3961 gcccgtcgct gtcgctgtcc gccagcgcat cggatcgcaa ccgcaaccgg ttgccgttcg
     4021 tggtgatgac gaagcgctcc acggtcacca gcccatgcgc atccacccgc aggtcgccca
     4081 tggccgcggg cgctgattcg ccccggggca cgttctccca gacgactccc tgtcccggcg
     4141 cgggcttgaa caactggggc cggcgcaccg tcaccgccgc atgctgggcg cccgcgcggg
     4201 atcgcagcac gatttcgtag ttcgtggccg tgtccacgat gtccccggcg aagtggttcc
     4261 agggacagga ccattccagg gtcttgttgt agaagttggt cagcggctcc gcgggcggcg
     4321 gcgcggcgtc gttcccgctg gcccgcgaga aggccgggaa actgatgtcc tttcgaaagg
     4381 ccagttgccg gatgtcccag ttcgtggtcg aggccgtcgg ccagtcggcg aacgcgatcc
     4441 cggaatggct ggacgggtcg atcagggcga caaatccgca tcgcgcctgg taaaatgccc
     4501 cgatcgccgg ctcgccctgg gtctcgtact cgatgttccc gtcgttcttg cccgcgtgga
     4561 agaagaacat cggcacgtcc cggccgggat gatgggtcag catggccagg tggtccatcc
     4621 agtcgaaaac gcccatgccg ttgaagttcg tgatcggccc ggcctgcggt ccgcgatttt
     4681 cgatgggaag gttgtaatta gttgcgcccc agagtgtttc aaaatcctgc ccgcgccaga
     4741 aatccgccgt cctgtacacg gtgaccggtt gccagcagaa caccgccgcg aacacgtcgg
     4801 gatagtgaaa gcccagcgtc atggcgccgc tcccgcccat ggacgagtac cacgcataga
     4861 tccggttcgt gtccacgcgg taatacggat cccggatgac atcgtggatc gcgcgcaggt
     4921 gacgctgctc ggtgtaattc acgatgatcc cgctggtcgg gtgcgtggat ttcgagtagt
     4981 cccagttttc gtggaagccg taatgccacg tgtcgtccgg gtcgtcgccg tagatctgga
     5041 tgccccggtt gtaccacgag ccggtctcgg tttcgtcgta ccggctgccg tgcgagacca
     5101 gtttcaggtg caggggccat gcgaccgtgt tcgagaagtt ggccggcagg cacacgctgt
     5161 agttgtaggc atagccgccg agcgtgggat tccaccggtg gtaatccatg tactgcgtgt
     5221 aggtccgcac ccggcccgtg gcgctggtcc ggacgaggac gggctcggga tcggcctcgg
     5281 cctccgagac ggcggcggtc agcgcgttgc ccgcgccgaa ctcgttggtg ttttcccggc
     5341 cgttggtccg gagcgtgacg gcgtagaaga aggcgccgct gtcgtgcggg gtgtacacga
     5401 agaggccccg gttggacgcc aggttcgggc cgagattctc gatgacgaaa tacacgttgc
     5461 tgccgcggaa cgcggattcc gcggggtagt aggaggatcc gcgggggacc gaggcgagca
     5521 gcgtggccga ggcaagcgtg cccgtcgaga tttccccggt gtgccggtag acccggtagc
     5581 tttccgccgt gtccccggcg ccggcctcgt cccaggtgag aaacgtctgc cccgaccggt
     5641 gaaacgcggc gaggttcgac gcctgctgcg cgcgggccgc cggccccgcc agcattacgg
     5701 ccaggcccgc caccgcgcat gagagagcaa gacgcatgga ctgggtatta atacacaaca
     5761 accgccccga tctcaaaccc ggcgcggcct ttcctgttga cccgcttgga cgggccggta
     5821 cactgccccg cacatgcgaa gcgcgtactc acaagtcggg tgcctggtcc tcctggcgct
     5881 ggtgtcgtgc gcgccgccgg cccgggccgg cacggtcctg ctcgagaatt tctcctatgc
     5941 cgaccccgcc gaggcgcaca aggcctggct gccgaaggac gcctcgccgc ccgtgacggc
     6001 cgtccccggc ggcgtgacgt tcccgtgtcc cttcgacggc ggcgcggatc gcgtctactg
     6061 ggaccgcagc gtggcgctga acctggatcg ctacaccagc ctggaactgg atctgacctg
     6121 cgatcatccc gaggccctgc gctcgctgac cctttatttc aagagcggcg acggctggtt
     6181 cgtctggggc aagccgctca agcgggccgg acggcagacc ctcttgctgc tcaaggagga
     6241 attttcgacc gagggccggc ctgcgggctg gcaccggatc gaagccatcc gcgtctcgcc
     6301 ctggcgcggc tcggcggtgg cggcctcgct gacgttgcac gccctgacgg cggccgagga
     6361 cagcctgctg atcgtgcggg ccacggcctc cgcgcaggac gcctccgagg aagccctctc
     6421 cgcccggatc gcccgccgct tgagcagttg gctgcgggaa ctcggcctct cgcacggcat
     6481 ggtgagcgat gacgacgtgg cggcgggcat cctgcgccgg gcgcgcgtcg ccatccttcc
     6541 ctacaacccg cgccccactt cgtccatgct ggccgccctc cggacgttcg tgcagaacgg
     6601 cgggcggctc gtggtgtttt atggatccga tccgaacctc gcggccctga tggaaatcaa
     6661 gctgggacgt tatgcccgca ccgacctgcc cggccgctgg agctccatga ccttcgtgga
     6721 tccgtccgcg cgctatctcc cggcccgggt cttccaggag tcgtccaaca tccttcccgt
     6781 cacgcccgcg gggaaggggg gcaaggtcat tgcgtggtgg gaggatgccc tgggccgccg
     6841 cacgcgcgac ccggcctggg tcgcgacgtc gcgcggactc tggatgacgc atgttcttca
     6901 gaacgacgac gtgcgcacca aacaggagat gctgctgggc ctgctcggcc ggctcgatcc
     6961 cgccctgtgg caggaagcgg cgcggcatcg gctgaatacc gccggccagg tcgattcgtt
     7021 cacctcgctg gcggatgccc gggaggggat cggtcgcctg ggcgccgcct ccgggcagga
     7081 agtgcagccg ctactcgaac gcgccgccgc gctgcatgac cagatgcggg cgctgtatga
     7141 cgagcagcgg ttcctcgagg tgatccaggc cggccggtcg ttgcgccagg tcctgaccga
     7201 ggcctacgcg cgcgcgcagc cgccgcgcgc cggcgaattc cggggggtgt gggaacacga
     7261 gggcgtagga tggtttcccg gcgactggag ccggagttgt cggttgcttt ccggcgcggg
     7321 cttcacggcc gtgtttccca atctcctgtg gggcgggctc gcgcactacc cgggtcgcgt
     7381 cctccccgaa tcgtacacgt cccggaaact gggcgaccag gccaagtcct gcgtcgaggc
     7441 cgcgcaccgc aacggcctgg aggcgcacgc ctggatggtc tgctggagcc ttcagaacgc
     7501 gcccgacgac ttccagctcc ggatgaagaa ggaaggccgc ctccaggtga ccgcgaccgg
     7561 gcagacgata ccctggctca atcccgccga cccgcgcaac gtggacctgg ccctggccgc
     7621 gatccgcgaa ctggtgacgc gctatccggt ggacggcgtg cacttggatt atgtgcgata
     7681 ccccggcccc gattcctgct tttcgccggt gtcgcgtcgg gaattcgaga aggaccgggg
     7741 gcggcgggtg gacggctggc cgcgcgcggc tgcgccgggc ggagcggtcg ccgcggagta
     7801 ccagcgctgg cgggcgggcc gcatcaccgc cttcgtgcgg gccgtgaacc gcgagatcaa
     7861 gaccatccgg ccgaccgcga aggtatccgc cgcggtctat agctcgtacc cggactgcat
     7921 cgcgagcgta gggcaggact ggggggcgtg gcttcgggac ggccttctgg atttcgtctg
     7981 ccccatgacc tacaccaccg acgcggcggg attcgccgcg ctcacgcaga agcacctggc
     8041 cctgcccggc gctgcggggc gcctgatgcc cgggctcggg gtcacggcgg atgagagcca
     8101 gttggcgtcc gaccaggcga tcgagcagat cgtcgccgcg cgcagactgg gtgctcccgg
     8161 cttcatgctg ttcgacttga gccacacctt gcgcgaggaa atccttccga tgcttcgcct
     8221 gggtctcacg agcccgcctt gattttgtcc gggccgaccg tcgctggccg gaatggccgc
     8281 tgcttcgctg ctcttgtccc gtccctccca ttgcgatgac cctgatgtcg tcctggtggc
     8341 tgtcctgaaa aaaacgtgga attctcgcgc gcgacttcct ttctgtagat ggacgcgcta
     8401 tgcgcgtaaa aaagaaggag gacagggcaa gcaacgaaac agttgtgaca taggcacgct
     8461 ctgatagaac gtgtcacaag cctgacggag cgggcatgaa gcccgggccg atgttccatg
     8521 cgggcgccag gtcgttgtcg gggcacgaat accgcctgcc gaaatatctc gaaacgcggg
     8581 tggacgggag gggagcggga gatactcggc acgggggggc ggtgtttgga cgccggggga
     8641 tggaagccgc gcccgaggtt gccgtggcga ccgggccgtc caaatgacag agggttgggc
     8701 cgtgcgccgg tcccggcgaa tccggtgcgg ccggtgttct gcggccagac ccgatcccgg
     8761 agaacatcgc caggtgaccg tgctttgggg ccagggctcc gcctggagcg ggcgatcgcc
     8821 cgaactgacg cgcctgaaat gagagcacgt cgaagcgggt gtgtgatcga ctcctggcgc
     8881 atccaggcgg ctgatgccgg cgacaggcgg ggcgccgcgg gtccgcgctg gacgggacgg
     8941 acaggcgtgt tggcgcagtc cgggcttgtt gtccattgcg caggatcatc ccgcagcgga
     9001 ttggtgtggc tccgacgcct gggcacgcgt ccattgcgtt acggggaaga tgctggcgtc
     9061 gtctgcagtt gagccgacac ctgcgcgatc cgctggatga tgggcgtggg cggttccatg
     9121 ccgggcggcg cgttgtccaa ggcaacctgt agcgcctgcc ggatggttcc cggcgtcacg
     9181 gtctcgttgt cggtcctcgc gccttggaag tcgttgtagc ggtcgaaggc cgtggtatac
     9241 ccgatcatga actgagcatc cacgtcctgg gcctcggtcg cggtgccgta tcggtgttcc
     9301 gactgccggc gcaaacccgc cgtccggtag aaccgccgtg gagcccgtag gcgtcgcccg
     9361 gcagcaccgc gtccaaaggg ctcttgttca tgagccggga atacagccac ccggcggcct
     9421 ccgtgaccgg gccgctcggc gctccggtca aggtggtcct ggtcttgtcg ggcgcaggcg
     9481 cggggatggt gaacatcccc ccggcgctta cgcggcattg ggatccgtcg ttttcaagaa
     9541 ccgagatctg cccgatcagc cggcctccgg aatcccggat caggattttc ctccgcggcc
     9601 cggctgcccg ccgtgagcag caggaagaaa caggccggct gtatgaaccg gctaaaagcg
     9661 ctggcgcgtc tgaacctcat ggggcggacc ttcctgcccc gtacaacgtg caaccccccg
     9721 ataaagtgca tcccggccac gggcgggctc aatttccgcg cgcgcgatac gttccgctgg
     9781 aggccgcgcc gcccggctcg cgccacagcg cggacacgcc catccccgag tcgaggagga
     9841 cgccgttggt gaccggccgc cagtccgggt tcgccagcga ggcgcaagtc tcgaccacgt
     9901 acccggtgct cgcgggcgcc gactgccgga agaaaaacgc ggtctggccg aaggcgtcga
     9961 tccggacatg gcggccttcc acgtccggac cgggcgactc ctgcgcgccg ggctgccaga
    10021 acaagccgag gctcttgccc gtgttgtaga cccggcccgg cgtgtcatag accggccggg
    10081 tgcccaggat caggacgttg gtgagcgggg cggccggcgg gaccagttcg ccgaaggtcg
    10141 cattgcgcag gatgggaaag ggggcggcat cgagcagcgc gcccccggcg tccacccgcg
    10201 cgccgaagag gccggccggg ccgttgcggg tgtcgcggaa caggcacaac caatcggtcc
    10261 cgtcacaggc gacggcgctg tttgcggaag acgcccaggc gttgatgacg aggaccgtga
    10321 tcacgttgcc gagcgccgcc gtggaggcgt ccagggtgcg gccgaggatc tggtaggcgg
    10381 cctggtcgcg ccaggtgacg aggaaccggt tgccgcccgc gtgataggcg gcctgcggtt
    10441 cccagcgcac ggaagacccc gagtacaggt tcgacgccga ggtgtcgatc gcggtcccgc
    10501 cggggcggta gcggcggaag cggatcgccg cgtcgtccgc gcacaggacg agatagcagc
    10561 cgccgccgaa cgtgacaaag gggtcggaga tgtcggagcc gccgatgtcg aacgcgccgc
    10621 ccatgctccc gtccgcccgg acaaagcgac cgcgtgtttt tcccgaatcg gaatcgtccc
    10681 agaccgccag gaagttggtg ccgtccgacg cgatgtcgca gaagtaggcg aacgtcgcgc
    10741 ccacgccgat ctgcacattg gttcccgccg gctgcccgtc cagagtcagg cgctggcccc
    10801 acacctggtc gcggggattg cgttcgtccg tccagaccac gtataccgtg tcccggccga
    10861 cggccgcggc gggcgcccac tggtcgctgc tgttggtgca gacgaggaag ggggcggcgg
    10921 ccagggggat gcccccgggg gaaacccgct ggatggcgat gtcgccgagc cgctcggaat
    10981 gcgccggatg gtactcgaag gccacccaga aattcgtccc gtcgcatccg cccgagttgt
    11041 aacgctcgcc ggcgcccgcg acggacgcga ggcgctgggt gccggcggtc aggagggaat
    11101 cctgcgcctc gatggcctga ccccagaccg tctcgtcgcc gtgggcggcc aggaacgagc
    11161 cgttggtccg ggcgcatacg accggcgtgg atgggtcgtt ggtggaaacc agcgtcccgt
    11221 tcgtgtcggc gaagcccccg cccgaatcca tcacgctggc ccggaaggcg ggcggcgagc
    11281 ggtaatcgtg ccaggcgagc aggtagcggc tgccgtccca ggcgagcgac ggggcgacct
    11341 ggtcgctagc cgcggtgatg aaggccatgc cggtggagga gtggctgacc gtgccgtcct
    11401 gcgccacgaa ggcgccatgg atgtcgaccg tcccgctgcg gctgtcctgc cacgccaccc
    11461 accaaccctg cgttccgccc ggccccggcc cgaaggccgg acaggggttg gcctgggtgc
    11521 ccgggaacac gcagatcgga aggggcgccg catccagcac cgcgcctcct tcggaaaggc
    11581 gcaggccccg gatatcgttg ctgatcgcga cggcgagcag gaagcttttt tcccccgccg
    11641 cgaagttgcc gtaggccagg cccggttgca cgtagccgga aaagcccgcc atcacctggg
    11701 tcgccgcgcc gacgggttgc ccggcggggg agcaggtttg cgcccacaac cggtacgtgt
    11761 tgctgtaggc gctccacgtc atgagcaggt tcgttccgcc cgcgcagacg caggagcgat
    11821 aggccgaggt cgcgcaggtt tccaccggga acggcgtcgt gtccgcgaat cggccgtccg
    11881 ggtacatccg ccggcccaag ccctcccgcg gcccgctgcc ccggtgctcc tgccagatca
    11941 cccagaaggc gtcttgaaac cacatgaccg cgggatagcg gcgctcggtg gcataggcgc
    12001 ccacgctgtt gggggcgatg agcagggcga ccgtgtccag cggccggccc tgcgcgtcca
    12061 gccgcatccc cttgatctcg tcggacccgt tgtccgccca cacggccagc cctatgctcc
    12121 cgtcggaggc cagccggtgc tcccacgcct ccgtgaactg tcccgaaaca tccacggcgg
    12181 gattaatcag gaacggcatg cccacggttc cggcctgcgc ggccaagacc ccgagaagca
    12241 cacccccccc tatcctgcgc cacagcgcgc cacgattgaa catgatcagc ctccacggga
    12301 aaaaagcaaa acgcgccgag gcccccgttg tccagctttt tccccgtgcc gggacgggct
    12361 ggcccttcgg atcggggcgg gaccggaagc ttgctttgcg gccgtggatt ccgtatgcag
    12421 ggatagcttt agagggattc atcatgtcca cgcgcgaccg cagccccctt gcgcttgcat
    12481 tgaccttggc gatcctgtgc gccgggagcg catccgccca ggcgccggcc gttacgcgct
    12541 ggatggatcc gcagggccgg ctccgcccgg cgcatgcgcc ggaggcggcc ggcgggccgc
    12601 tgaagatgac gttggcccgg gttccgtcgg ctcggggcgc gccgatcccg ccgggagcgg
    12661 agaccaaccg ggtctgcctc gtcgtccaca ccaacgtggt cggggcgctc ggcgcagccc
    12721 tgtcccggta tcaggcggac ctgggcgccg cggggtactc ggtcctgctc tacacctacg
    12781 aaagcggcag cgcggaaaac ctgcgcgcgt acctggccgg actgtacgcc cagccgaact
    12841 cgctggccgg cgcggtgctg gttggcgaga tcccgtacat cgtgtacgag atgtaccagg
    12901 actggggcaa cggcaacgag tacgaggatt ttccctgcga tattttctac gcggacctca
    12961 acggcacgtg ggccgacacg aacagcgcgc cgcccttttc ggcgggcaag tacgattccc
    13021 ggagcggaaa cctcgacctg gaaatctgga tctcgcgcct gaaggccggc aacctgccgg
    13081 cgctgggcgc ggagaccgac agcctcgcgc gctatttcga taagaaccac cgctaccggg
    13141 agcggcaact cgtcccgcac cgccgcgcgc tggcctacgt ggacgacgac tggagttacg
    13201 gcaccggcga ggacgcggag tgcctgggct ggcgctacgg cgacgcgggc gtgcgcgccg
    13261 tgatggacgc cgactccacg acggccgccg attacatggc ctttatgacg aacagctacg
    13321 agctgatgtt cgtccgctcc cacgggtacc ccggcgggca cggcttctac gaaagcggga
    13381 agaccgcctt taactatgtc ctcacggacg actacctcga tctcgcgccg ccggcgctgt
    13441 tctattcgtt cttcgtctgt tccggctgcg actttgccac cagcgacaat ctgggcagcc
    13501 ttgtcgcgtt taatgccgac gactccgggc tggcctgctg gggcagcacg aagacgggcg
    13561 ggatgtggga tgaccgctat ttctacgagc cgctggcgga gggcgccagc ctcggctcgg
    13621 cgttcaaggc ctggttcaat gtctgccaga ccaactggcc ctcctacgcg ccgccgtggt
    13681 ggtatggcat ggtcatcctg ggcgacgggg cgctggcgtt gagcgggacg gagccggcgg
    13741 gttccgccgc ctcgggttgg agcgcggcgc cggtcgtgtc ctcgccgtcc gtccagggcg
    13801 gccgctacca gctccgccgg accgcctcgc ttgcggcgcc ggcgtggacg aacgtcggca
    13861 gcatcgtgac cgcggcccag ttcgcggtgt cgctgtcgga caccaacgcc cttgtcgatt
    13921 tcgcggcgta ccgggtcgtc aagttggagg acgggacgag caacctcctc cggaacgcga
    13981 gttttgaaat gccggggtca tccgacagcc gcgcgcgcta ctgggaggac gccaacccgg
    14041 attcgcacgg caacatctgg agcaatgttt cgcgggtcgc ctggcgctgc tactacggcg
    14101 gggcggaggc ggccgtgcgc ggcacgtggg cgggcgtgaa catcgggggc tggtggcagg
    14161 aggcgcccgc gaccgcgggc tccaattacc aggcctccgc ctggttctgg gccgaccact
    14221 ccgccaacgt ctggacggcg gccgtgcagg agctcaagat cgagttcctc gacagcggct
    14281 actcgctgct gggttcctcc agcgtggggc tttccggcgt cgtcgagagc tggacgcaga
    14341 agaccgtcag cgccgtcgcc ccggcgaaca cggcctgggc gcggttcgtc gtcaacgtga
    14401 gcggcgcgtc cgcgcagggc gcgctgcagt tcgacgacgc ggaactggtc cggagtcctt
    14461 gatcactcgt cccgcacgcg cacccggtag agccgcggcg cgccggcggc gggcggcggg
    14521 gtgagcgtga aatcgtccgt gaacgaccgg ttcgtggccg cgcccttggg cgccgggtcc
    14581 atctcctccg cgagcgatcg ggcaatgtgc tggaaatcgc ctgtgatccc cgaactgtat
    14641 tccacgccgt agcgggtgcc gccgaccgag ctccaacgca gcgcgtagcc ctgcgccgcc
    14701 cagggctggc cgaccagccg gagcaccgag ttgctgtcgt tcgggtccgt gttgcagcgg
    14761 tactcgtcga ggttcggcat cccgtcgccg tccgcgtccg ccgtcgccga gccggccgtc
    14821 gcattcgtga actgcgcggt ctcccagtcg tcgggcatcc cgtccgcgtc ggaatccggc
    14881 ggcgcctgga ccgcgacgcg gagggccgcc ggcgcgctgc tggtgagccc gtccgaggcc
    14941 acgaaatcga acgagtcggt gccggtgtag ccgtgcacgg gcaggtagac cgccgtccag
    15001 gtcgaggtgt cgacggcgga gaggctgccg tgggccgggc tgccggccgt cttgaacgtc
    15061 agcgactgcc cgtcgggatc ggtccccgtg agcgcgaggt tggtcggccc gtacccgacg
    15121 tgcacgacct gtgtggccgc ggcgacgggc ggacggttgc tgaaggccgc gcactcgcag
    15181 ccctgctgct cgtaggcgat ttcgagcgac cagcacaggt tcgtgccggt gttccgggag
    15241 aaggcgtcgc gcatgcggag ccgccacgtg ccgttgagcg ccttgccgtt gaaggcgtcc
    15301 agtgtgctct ccggccggta cgccccggca aacggggggc tgccgttgct gatggccagc
    15361 gccgcagcct ggtcgaggac ggtgtaggcc gccgatccgc acgtccccgt cccgaagtgg
    15421 tcgccgctgc cgccgcgccg gtagaccagc aggacctccg agccgtccgg atgctggagc
    15481 gcgagcgtga gatcccggtc gtaagtgtgg tgaatccgca gccgcacgtt gacgtcgctg
    15541 accacgttgg tgcccggctc gctgatcgtc aggtcggaat aggtggtcga ggcgtcggcg
    15601 atcggtttgg gcgtgtccgt gctctcgaac acgttggtcg tcgtcgccag gtccacgaac
    15661 cgcccgatca cgtgctcgaa cgcgctggtc agcgcctggc cgttgaacga catcgccagc
    15721 tcgaactgga ccaggctccc gcacgccagc ccgggatcga gccggtactc gtacgccgag
    15781 gcgttcgtcg ccgcggcgtc gggggccatg tcctcgaagc tcgatgtgcc ctgcacgatg
    15841 gtcacccccg gcgtcgggga ggtgagcgcg acgctgacgt tcgtcgcggc cagctgggac
    15901 ttgttccaca aaacgacgtc ctcgcggatg gtctcgccgg ggtcgaggat cccgtcgtcg
    15961 ttgccggccg gccggtcggc gggcgcggtt tcgcggatca gcatgcccgt ccgcggcacc
    16021 tgggagagga ccagcacgct gtagggggcg atggtgatgg tcccccacgg cggcgcgccc
    16081 gcggcgacga cctcgacgct gcccgcgttg ccgtagtcgg cggcgtaatt cgtggagtcg
    16141 ctgttgaagt ggacgtacca cgttcccgcg gcggggaagt tgatcgggta gccggaccgc
    16201 gtcgcgttgg cgaagttggc ggcgaccacc gtgtagcggt ccgtcgccgc ggaatcccac
    16261 cggctgtacg ccacgagctt gctggcgttg tccacgtggt aggcgctgca gctgtcgccc
    16321 agcaggccct ccgacacgcc gtcgaggttg cggcgcagtc ggatgaggtc gcggtagagg
    16381 cggacgatgc cggcgaagct gttcgtgcgg gtccagtccg cgcccaccgt gtcgctgaac
    16441 tggttggtct cgagcatctc ctggccctgc aggatcatcg gcgtgcccgg ggcggtcagg
    16501 accagcgccg cgccgagcgt cgagcgcttc cgggcgtagt agccggccgg gtcgcccgag
    16561 tggatcgccg tgggcaggcg gacgccgccg ttgaggtcgc cggccgagtc gtgcgagtcg
    16621 gtgaagatca cgcgctggaa ggtcccggcg acgttccggg cgacggtcgg catgtcgcgg
    16681 ttcgagtcgc tgccctgggt gagcacgctc cggatctccc agaggaaggt gaagtcccag
    16741 taggcgttga atccgctcaa ctccttcacg tcctccgcga tgttccacgt gccgggatac
    16801 tcggcggcca tcatcgcgag ggcgttcgtg atcagcgtca gcccctccgg gatgtagacg
    16861 ccgttggtcg tgtagaggat atggttcggc gcgtcccagc ggaagccgtc catccggtac
    16921 tcgttcttcc acatccggaa cgtgtcgaag atcgccgcgc agacctggtc gttgctgtag
    16981 tccggcctcg ggccccacgg cgtcgtcgcg tacgggtccg tgttgtagaa gtactggccg
    17041 ccgtggacgc cgtccgggtc ccagccgtcg aaccgccaca gggacgagcc ggaatcccag
    17101 tggttgtgga ccacgtccag caggaccgcg aggcccccct ggtggcactg ccgcacgagc
    17161 tcccggagcg cgtccggcgt tccgtagtcg tactcgacgg agaacgggta accgggattg
    17221 tagccccagc tcgtggacga cgggaactcg ttgacgggca tcagctcgat gccgctgacg
    17281 ccgaggttgc gcaggtggtc gagccggccc gtgaacgtcg agaacgtgcc cgacggcccg
    17341 ttgaaggtct ccgcgtgcgc ctcgtagagc acgaggtccc gcgaattggt gaggctgtag
    17401 gcgtcgccct gccaggtgaa gttgctcggg gccgtgatga ccgagttgtt gttgtccccc
    17461 gcgttgacgc gccggctgcg cgggtcggtc ttgtaggtcg tcggggtgtt gaccaggaac
    17521 ttgtagttct ggcccgtcac cgcgccggac acgtccgcgg accagacgcc cgaggtcgcg
    17581 ctctccaggg tgagcgggcg cgaggtcgtg ctccagccgt tgaactcgcc ggcgacggtc
    17641 aggttcgtcg cgtgcggcgc ccagacccgg aacgtcacgc ccccggcgta cggcgtcgcg
    17701 ccccagccgg gccgcgagga ttgggcgggg gcccgcggcg cggaacagag aaaagccagc
    17761 aacagccatg cggcgcggta tgacatgcgt gctccggaat ccgcagtacc tgtacgccaa
    17821 ggcagagggc ctttcaatcc ctgaagcgtc acggagggga cggcgcggtg ccgcgatgtc
    17881 cgcctcggaa ccgggatggc ccgcctgtgg gcccggcgtt catacggcca ggtcctcctg
    17941 cccgctgggc cggggctcct tgcctccgca aaagagcgac aagccgaacg ccagcgggcc
    18001 gatgcggccg acgaacatga gcgcgatgac gatgagcttg ccgacatgcg tcagctggga
    18061 agtgatcccg gtgctcaacc cgaccgtccc cagcgcggag gccgcttcga aaagaaggtt
    18121 ctcaaagcca tggctttccg acagggcgag caggaagctg cccaggatga gcgacccggc
    18181 gtagaacccg agactggcca tcgccagcca gacgcgggcg gccgggattt ccttgcccca
    18241 gaagtagacc cgctcggcgc ccttcatggc gctgcgcatg acgcccagga tggcggtgaa
    18301 ggtggtggtc ttcaagcccc cgcctgtccc ggagggggag gccccgacga gcatcaacag
    18361 gatcatcagg aaaagcgatg cctgggagag ttgcgagatc gggatggagt tgaaccccac
    18421 cgtggtcaat gccgccataa cctggaagag ggatgcgagc agccggtcgt cggtgtccag
    18481 ggcccgaatg ctcggttcgc agatgaaaaa caggagcatg ccggccacgg tgagccagaa
    18541 cgtggaaatc aggatgatcc ggctcgtcag cgtgatgcgc ctggcccggc ctgtgacgcg
    18601 acgccagaca tccaggcaca caatgaagcc gacggccccc aggtagctca cgatcccgag
    18661 aatggtgttg atccagaaat cgctccggta ggcttccagg ctgttgtcgt agaggctgaa
    18721 tccggccgtg cagaacgccg atatgctgtg gaatatcgag gaccatgccg ccgcctggac
    18781 ccccgcgtgg cggaaggcgc cgtagagcgc cgccgcgccg gccgcctcga tgatcgcggt
    18841 gaacacgatg acgcttcgca gcaggtgatc gatgtgcagg tccgccggca cgctgaagac
    18901 cgtgcgcgcg atttccctcc ttcgttccgg caacgcgccc gttcgggaca ggaccacgaa
    18961 ggagccgaag gtcatgtacc cgatgccgcc gacctggatg agcagaagga tgatggcttg
    19021 cccgagcagg ttgtaggagc cggacgtgct caccgtgaca aggccggtcg ttgaaagcgc
    19081 cgaaacggac gtgaacagat tgtccagcca tgtaacggac ttcgcctgca ccacgggcag
    19141 gcaaagaaca atccatccgg ccacggcata cgcaaggtac ccgatcgcga cgaggcgcgt
    19201 aggggagagg cgtccccaaa acaccgccaa gcccttgagt gatcttttca tgctgttctc
    19261 gccttcctgt cgaacaagag attgtccttc atgaaaaagg ggaactgcag caacctgcaa
    19321 aggtcaacgc gggcgataat acagcgctta ttccttccgg atttcaatct atttcccgaa
    19381 tcggggcgca acaggccttt tttcaaggtg ttccggcccg ccggcccacc cgcggaatcc
    19441 cggcagggcg catccgagtt cggccggacg gcgcccgttg agagcggtct gaaagcgcca
    19501 tgtgtttagt gcgcttatgt cgaatgaggt tgagatgcaa aggccaccaa tgggccttaa
    19561 tcgcggcggc gaaggcagaa gtgccgcgag ccgaattgga tcggatgatg acctgcgccg
    19621 gggcggacgt atcggaggcc ttatgacacg ctatccgctt tacacctcct gcagcgggcg
    19681 tatcctagtg ccagcatcag gagtggttct gccgtggagc agagcctgcc aaccggggac
    19741 ggaatcctgc ggtggctcct cgaggcgaga gcctcggggg atgtgccccg ataaagagtc
    19801 ggggaaacaa taatcccggg cgaagaaggc catcctgatg cgggatggcc ttcttgtttg
    19861 ccgagttcct ggtgcgctgg aaggaaggcg cactatggcc cacagaagca gcatccggac
    19921 gaactgggac aatccagacc accatatctg gaacaacaac ggcacgtggt tcgtgcatta
    19981 cacgatccac cggccggact acaccaagga gcgcatccac cgctcgctgg ggacgcgcga
    20041 ccgggccagg gtggacgttg gtgtcgtgca tgaccaccac gccgccgggc gacagccgtt
    20101 ccacgtagcg ccagtcggcc aggcgctggt tcacggagtg ccagccgtcg atgaagagga
    20161 aatcgatggt gtccaggtcc agcccgtcca tcagggcata ggcccggtcc cggtccgccg
    20221 aatccatttg gagtgtgcac acgcgcgcgc cggcgtcggc cagatggcgc ttgtcctcga
    20281 tgtcgatccc aatgtacgcg cagcccgcgg gcttgtgccg caggaggatg cccgtcgagg
    20341 actccggcag cgggttgcgc tggaccccga tttcgacgat gcagagagcg cggcgcgctc
    20401 tgcagcgcct gccggagcac gtcctggttg gcgggcgaga attcgcgcgg gacgtggcgg
    20461 tggaactcgc cgtagaaatc cgtgttgtcg tcgcgctcgg gctcgaggct gggccggtac
    20521 atcaggccgt agatggactc cggtcgctcg tggatggtgt acatgttcat ctctccaggg
    20581 tcggtgtttc agcggagatc cggcgtcggc cgaatagggc gatcgccagc gcgagcccca
    20641 gcccgccgag cagggcggcc gcggtccggg ccgggacgcc cccgttgccc cgcgcgggcc
    20701 cggcccaggc cagcgcggcg cacgccgtga ggttataggc tggcagggcg gctcccgcga
    20761 ggcggccggt ggcgctcccc ccgggggaaa tccggagcag gccgtacgcc acggccgcag
    20821 gggtggccag cagcgccagg cccgtcgcga tttccaggac gccgcaggtg cccaaccaac
    20881 gggccagccc gtccggcttg ttcatgacag ggacctcatg tccgccggct ggtccacggt
    20941 tcgggccgtc ctgtcaatcc gcggcagggt cggcgtcgtt tggcgaacac atgaagtcgc
    21001 aaggcggtat ggctaatccc ctgccgcaca tggcggcggc actcaacgga cgacgggaga
    21061 tgaaacagtc ccggccggtc aaggcaatcg gacccgtgtt cgatacgaac gaaaaggcgc
    21121 gttgttggtc aggatgaata ggatcgtccc cccgacgccc tgttcctcgg gcaggatcaa
    21181 agcccagtcg ccggcgcgca agtccacggt tgcttccacc gcgtacatcc ggccggtcga
    21241 tgtctggggt atcgccatgt cggccgtgaa tccgaaatct ccggcgaaag cgacccgcgg
    21301 gaagaacgac gcgccgttgg ttggattcgt gtcggcgacg tattcacgag agttgatatc
    21361 gccgtccagg tccgtatcca ggccgtctat ggcgtcccat tgtgcttgat cccatgcttc
    21421 gcccgcggcg gggcccgccc ccttgagccg ataccatgcc gtggccacgc ccccggtggt
    21481 aaaagccggt tcgcggaaca agtacgactg atttgcgttc tggacctgtc cccaatagga
    21541 gcccgcggga agcgtcggca agtcactggt ggtaaacgag ccgctacggg cgccatgaac
    21601 catgaaagca aagatgcttc cctcggcggg gaaatatcct ccgtccagcg tcacccgaag
    21661 cgtcccgtcg accgcggcgt tgccggtgac cacgagttgg tcgtgcccgc tcccgggcgt
    21721 tgtgccgccc aactcgactt ccagcacgcc ggacgacgcc tgtgcaaaat gcccggttat
    21781 cgtcaatctc ccgacagaac tgcccggggc ggccgtcccc cggttcagca ccgtggccgt
    21841 gcccagggag atggagccgg cgccccgcat gacgccgtta ttggtaaatc cgccgctcac
    21901 ctggatcgtg gcgccggtgc ccgcatccag gatgccgtcc tgcatgccca ggacggaaag
    21961 catcagacat cccgagcggg aagccagcac gccgcccgtt gcgttcacaa acggcggctg
    22021 aacctggctg atgccggccc cgtcggtttt cagaaaccat ccagcattcg ttacgacgcc
    22081 cctcgcgacg ttgaatgtga tatcggaatc gtcctcgatc tgatagatgc cgcggttatc
    22141 cagcaccccg ccccgattca cgtaaaaaga acctgatccg gtgtcaaact gtcgcagcct
    22201 tccgtggttc tccagcgcgg ctcccagatg cttgttcggc gtcgcggtgc cgctgatcgt
    22261 atttgttgtg cccggcgaca agaccatcct gcaggcgccc gtcatgacgc ccgccgtcca
    22321 cgcaagggat tgggtcacgg tcagcacctc agcacccgtc agggttcccc cggcaagggc
    22381 gaaacatcct atctgcgagg ctcctgtgac cgacaaaaag ccgccggcca aacggagcac
    22441 gcccggtccg cccagggcgc cgcccatgct tccgttgccg gccgtcacgc tgaccgttcc
    22501 cgtgctgatc gcgcccgtcc cggccgcgct gaacgaggcg ccgaaagcaa ggcccgtgga
    22561 agaggtgctc ccgatgaggc ctccgtcctc attccggaaa tagacccgga tctcgctgcg
    22621 gccgctgccg ccggccttcc ggaatacgcc gtgattccgg atctcgcctt gcgaggtatc
    22681 ccgaacgatg caggaatcgt cttcgatctc gtacgaaccc cagttgtcca ggacgccccc
    22741 ggcgtaaata aagaaggccg tatcggcggt gccgaactgt cgcacggtgc cgcggttctc
    22801 cagcacggcc ctcaaatgct tgttgggaac ggccgtgcca ctgatcgtat tcgttgtgcc
    22861 cggcgccagc accacgctga aggcccccgt gatgaccccc gcggtccacg ccaaggattg
    22921 agtcacggtc agcacatcgg cgcccatcaa ggttcccccg gccaaggtca tgcaccctac
    22981 ctctgacgcc ccggtgaccg agagagagcc cccggccaga cggagggccc cagggccgcc
    23041 cagcacaccg cccagcgtcc cgcttcccgc cgtcacattg accgtccccg tgccgatcga
    23101 gcccgtcccg gccgcgctga acgaggcgct aaaagaaagg cccgtggaag aggtgctccc
    23161 gatgaggcct ccgtcctcat tccggaaatg gacttggata tcattgcggc cgctgccgcc
    23221 ggtcttctga aacaccccgt gattcctgat ctcgccgcgc gttccaaacc aggcgatgta
    23281 ggaatcgtcc tcgatctcgt aggagcccca gttgtccacc acgcccccgg cgtagatgta
    23341 aaaggagccg gtgcctgtgt tgcgttggat caaccgcccg cggttctcca cggggcagat
    23401 caagtgcttg ttgggtgacg cggcgccgct gatgacgttg accgagcctt ccgacaacac
    23461 gatcctgcca aggccatcaa tgtcattggc tgtccagttg aaagaaccat ccaccgtcag
    23521 ggatccggcc ccgcgcaaca aggctccgtg caggttcagc acgcccgcca catcgccggt
    23581 ggtggtcagg ttaaacgtgc cgttccggag ggtcagttcg ccgccgaccg tgagccggtc
    23641 cacggtagcg ccggacgtgt tggtgcaact cggcgtccct gccggaataa gcgcgtggtc
    23701 ggacgcggtc ggtaactttc tgggcgacca gttgttggta tcgccccagt tggcggaagc
    23761 cattcctgtc catgtggcat cttccgcgcg gacgggcgtg ttaccccgca acgcgccgat
    23821 caccgccgca gaccacagca ggaccgttgg gctgggtgtt ttcatcgaac cctccgggat
    23881 gcgagacgcc ttgccgtgct ggaaaagcga ccttccttca ggtggatacc catgggcacc
    23941 ctccttcaac aacgtttatg atagcacaac ctcgccggat gagtcgaggc gcgcatgatg
    24001 aatcactaaa ggaggttgtc gcgctcgctt gtggggatga cgcaaggggg cgacagtccc
    24061 gaacagatcg ttagtgatgg gcctgccgac ggcggggatc gcctacggcg agacagcctg
    24121 ctggattcga ggccggagcc gtcctgcgcc ggaattaagg aacttcttgc gtgcggcggg
    24181 cgcgaccggc acacacagga taacgacggg cagtcccacg gcttggggaa tggtggggga
    24241 aggattcgaa ccttcgaagg cgtagagccg gcagatttac agtctgctcc gtttggccac
    24301 ttcggtaccc caccggggga aaaccggaga catgaaagcg gaaaccggaa aataatcaag
    24361 ccctatccga tcagccgcag ccagatcgcg cccagcggcg ggagcgtcag cgggaccgac
    24421 cacgggcggc ccatccacgg gatctcgtcc gtgttcagcg tgtgcacgtt gaccacgttg
    24481 ctgccgccga agcaatccgc gtcggtgttg agcacctcct cgtacacgcc gggcaggggc
    24541 acgccgaccc ggtagccctg gcgcggcacg ggggtgaagt tgaagatgca caccaggatg
    24601 tcgcgcgaat ccttgccgcg ccggaagaag gacacgatgc tctgctccca atcgtggaag
    24661 tcgatccact cgaaaccctc ccacgagaag tccacctggt gcagcgcgcc gaagtcgcgg
    24721 taaagtttgt tcagcgtgcc gacgagggtc tggagcttgt ggtgcgagtc gtactggcag
    24781 aggtgccagt cgaggctgtc cgcgtggttc cactcgcgcc actggccgaa ctcgccgccc
    24841 atgaagagca gcttcttgcc ggggtgcgcg tgcatgtagg cgtagagcag tcgcaggttg
    24901 gcgaacttct gccagaggtc gccgggcatc ttgtccagca tcgcccgctt cccgtggacg
    24961 acctcgtcgt gggagaaggg caggatgaag ttctcggtga aggcgtagag cagggagaac
    25021 gtgatgtcgt tgtggtggta cttgcggtgg acggactcct tggtgatgta ctcgagcatg
    25081 tcgtgcatcc agcccatgtt ccacttgaag tcgaagccca gcccgccgag gtacgtcggg
    25141 cgggagacca tcggccacgc ggtggattcc tcggcgaagg tcaggaagcc ggggaactcg
    25201 gcgtggatga tctcgttgaa tttcttgagg aagtccacgg cctcgaggtt ctcgcgtccg
    25261 ccgaactggt tggggatcca ctcgccctcc ttgcgggagt agtcgaggta gagcatgctc
    25321 gcgacggcgt ccacgcgcag gccgtcgatg tggtacacgt cggcccaata caaggcgttg
    25381 gccagcagga aggtgcggac ctcgttgcgg ccgtagttga agatcagcgt gccccagtcc
    25441 ttgtgctcgc ccttgcgcgg gtcggcgtgc tcgtagaggt gcgagccgtc gaagtaggcg
    25501 aggccgtgcc cgtccttggg gaagtgcgcg ggcacccagt cgaggagcac gccgatcccc
    25561 tcctggtggc agcggtccac gaaatacatg aaatcgtccg ggttgccgaa ccgcgaggtc
    25621 ggggcgtagt agccgatggt ctggtagccc cacgagccgt caaacgggtg ctcggagatc
    25681 ggcagcatct cgatgtgcgt gtagccgagg cccttgacgt acgggatcag ctggtcggcc
    25741 atctcgcggt acgtcagcat ccggttgcct tcctcgggca cgcggcgcca ggagcccggg
    25801 tgcagctcgt agacgctgat gggcgactcg tgccagttag cccggccgcg gcgctccatc
    25861 cactcggcgt ctttccacga gtgccggtgg atgttccaga ccaccgacgc ggagcgcggg
    25921 cgcagctcgg agaagaacgc gtaggggtcc gccttctgcg ccaggaaccc gtggtggccc
    25981 ttgatctcga acttgtagag gtcgcccggc tgcagcgccg gcaggaagat ttcccacagg
    26041 cccgagctgc cgcggtgctg catcgggtgg tggcgcccgt cccagcggtt gaaccagccg
    26101 atcacgctga cgcggatcgc gttgggcgcc cagaccgcaa agtggacgcc ctcgaccccg
    26161 tcgatggtca tcgggtgcgc gcccatcttc tcgtaggtcc ggaagtgcgt gccctcgccg
    26221 aataggtaga ggtcgaagtc ggtgatctgg agcgggaagc ggtaggggtc ctcgagctcc
    26281 cactggtgcc cgtcgtaacc ggtcatgcgc agccggtagg cgaagggggc gcggtcggga
    26341 aagaacacct cgaagagccc gtcgttgtgg atgcgggcca tctcgtgggc cgcgccggac
    26401 tccttctcca gcagttcgac gcgctgggcg tacggctggt aggaccgcgc gaccgcgccg
    26461 ggcatctcgg cgccggtggc gtgcatgccg agcacgtcgt agggcgagcc gtgcgtgccg
    26521 tgcaccacgg aaagaatggc ggcttcgtgc agaatgggtt tcatgtaggg tccctcacaa
    26581 gagttgccgc attgtagaaa aagagccgcg aaattcaagc ccgcatccgc gcccgcgccc
    26641 gtccccctcg atcgccctct ccttctcttg cttcttctcc ccccacacat cggtccacgg
    26701 agaggaggag cgagaaggag aaagagatgg aggaggagca ggaggaagag aaagaggcgg
    26761 aggagttttc gtattccccg cttgccgggg aacgccccgt gcgggaagat atccaagcca
    26821 tggctcgtcg ccgaaagagc ttcaaccggg gcgcgtgggc ggtgcagcgc gagcgattcc
    26881 gcctggaaca ggccgcgccg cccgcggatt tcgccgtgga gtccgtcgcg gatgtcctgc
    26941 ccggcgtcgt caagaaactc ggcctcgccg cgccgttctg gatggagagc ctgcgccgcg
    27001 actggccctc catcgtcggc ccggacgtgg ccaagcgatc gcgaccgggc cacctggaaa
    27061 ataagacgct gaccatcttc gtcatccatc cgacctggct catggaactc aagcggatgg
    27121 agcggcaggt gctcgagata atccgccagt cctgcggccg ggagcaggtg cgcgccgttc
    27181 gcctggcgct cgacccggac gcgcgctgaa ccgccgcggc attctctgat attgccaagg
    27241 aggggccggt cgtttcatta tgcccgccgt tcggagcgat gccccggtag ctcagatgga
    27301 cagagcgctg gattcctaat ccggaggtcc cgagttcaac tctcggccgg ggtgcccgcc
    27361 cggcaccggg aacaatcatg agcacagccc ccatactttc cggcggcgcc gcccgcgagg
    27421 cggccatccg cgccggcgta atcaccggcg ggatcgatac cgtcatcacc ctcggcgcca
    27481 tggtcgcggc caactcggcc gtgctgctgg ccgacttctt caagaccttc ctcgagttcc
    27541 tggccgtgct gctctcctgg atggccattc gccggatcaa ccggggcggc ggggcgacct
    27601 acgagtacgg gctgggcaag ctggaaaacc tcggcagcct gctcgtcggc gtgctgatga
    27661 ccgtctgcct gctcatcatc accggaaacg ccgtgcgcaa tatgctgcac ccgtcgcata
    27721 tccgcggcct cggcgtgtgg atcagcatgg gctcccaggt gctctactcg gtgatcaacg
    27781 gccgcctcgg cctgcgcggc tggcgcatgg cgaaggcgga ggcgtcgccc ctgatggcct
    27841 cccaggcccg cctgctgctg acccgcgccg tcgccaacgt gttcatcctg gcctcgctgg
    27901 tcttgagcgt ggccttgagc cgcttccact gggcgcagta catcgacccg ctgtcctccc
    27961 tcgtgatcgc cgcctccatc ctgatggccg cgctcgggat cttctcctcc tccgtctccg
    28021 acctgctgga ccgcacgctc gaggagtccg atcagctggt catcctgcgg gagctggcgc
    28081 gctacttcga cgagtacgag gccctgcacg gcatccggtc tcggcgctcc gggacggacg
    28141 tgtacatcga tatcctcctg gagttcaacg ccgagcgcaa gatgggcgaa atccacgagg
    28201 tggccgagca gatgcgccgc cgcatcgagg agcagattcg cggcagccgc gtcactatcg
    28261 gcttggcgcg gacgggcact ccggctcctt aggacttcac cgcgctctgc gctcccggca
    28321 attcagcccg ggttaccggc cgcgttccag ccggtgcacc tggtcttcca ggtcgtcgaa
    28381 atcccgggcc agtttgtcca gccgcttctc gatgtcctcc agccggcgct cgatgctgtt
    28441 gaaggccgtc ggccggctgt aggaatcgcc gaggcgttcc tggatttcct tgatcgcctt
    28501 ttgcaggccc tggacctgct cggccagttt ctggctcaac tggtccaccc gctgcgccag
    28561 cgcggccgcc gggtcggccg gtgtctccgc gggcgcggtc tgggcctgga gcaggacggg
    28621 cgcggcggcg agggcgatca acgcgacgag cagtactttt ttcatgcgcg gctcccttgt
    28681 tggtgccgcg ccatacctac cacgccgccg gccgggccac aaggtcggag ggttcagggc
    28741 agcgatccgt gcgcgcggta gaagcgtgga ttgacgccca cggcggtcgt gtccgtgtag
    28801 atattgaccg gcacaaccgc cgggatgttc gtcgccaccg cggtccacgc gccggtcagc
    28861 aggttggtcg tgcggtcgag gcggtacacg cgctgggaga cgctggccca gcgcacgatc
    28921 cagccgttgg cggaggcggg cgcctgcgcg gcctgcgcca tcatgtgcag gctcgagccg
    28981 aaatcggacg ggtccgtgcc ggtggcctgc tcgccggtat ccgtcaggcc gtccccgtcg
    29041 gtgtcctgcg ccgcgccggg gccgaaccgg gtcaggaacg tcgcggtccc gccgttgttc
    29101 gtttgtccgt cgaacgcgcc cgcggtgaac ccgcagacat acacgttgcc gagcgcgtcc
    29161 ggcgcgacgt cgctggcctg gtcccacgac gcgctgcccc agatccggtc ccaccagcgc
    29221 gtgccgtccg gcccgaactt gctgacgcac gcgtcgaaca tgtagctcgc gttggtctgc
    29281 ccgccgaaat cgccgtgcgt gcggccgacg acgtacaggt tgcccgcgtt gtccagcgcg
    29341 gcgccgtacc cggtgtcctg cgcgggggag ccccagacgc gcgtccagcg ccactcgccc
    29401 gccgagtcga aggccatcgc cagcaggtcg tcgctgccga tcgccggctg gccgtccgcc
    29461 ccgccgtaca agtagcccgc cgcgtacacc aggccgtcgc cggacaccgc gatgtccatc
    29521 gcgtaggccg ccgtctccgg gccccggatg cgcgtccaga gcttctgccc ggcggcgtcg
    29581 aacttcgtga cgcacacggc gacgatcccg ccgttgagct gcccgtcgaa cgaaccgtcc
    29641 gtgtaacccg cgacatacac gttcccgctc ccgtcggcgc cgacgccgcc cgcccgctca
    29701 tcggcggacg agccccagat tcgcgtccat tgccgcgtcc cgccggcgtc gaacttggac
    29761 aggcacaggt cggagttccc ggcgttggtc tggccgtccc acgcgccgtc cgtggtcccc
    29821 gcgacgtaga agttgcccga gctgtccgcc gccacgtccg ccccggcatc gaacgcggcc
    29881 gagccccata tccgcaccca ctggacggtc cccgtggcgt tgacgcgaac caggcacagg
    29941 tcgcccgcgc cggagttggt ctggccgccg aacgcgctgt acgtcgtgcc tgccgcggcg
    30001 aagccgcctc cggccacgcg agccaggcgc tggccgaaat cgtggcttgt gccgccatgg
    30061 aggcgcaccc acaggctgct gcggctcgcg ctgtaggcgt tcagcaggat atcgcccgac
    30121 ccgagcgcgg gcgcgccctc gacggattca tggctgtcgc cgaccagcac gggccggttg
    30181 tccgcgtcca cggccacgcc attggcctgg agcccgtacg ccgtgttggt ggaaggccgg
    30241 atcacggtcc actcgcggac gggcccgtcg tgcacggcgt gcgaggcggg gttgagcggg
    30301 ttcgagccgg tgccgacttc gtagccgtcc gacaggccgt cgccgtcggt gtccgggttg
    30361 tccgggtcgg tgccggtgcc ggtttcctcg tacgggtcgg acaggccgtc caggtcgcta
    30421 tcgggctcct ccagccggat gatcacgttg gtcatgtttg tcgcgggccg gaacggattg
    30481 cgttcgtagc ggcccacagg ctcgccgtag ttccagatgt cgttgttatt cacgtcccgg
    30541 aacgcgaaga gctcgtacca cgtgtcgttc gggagcgcgt cgaactggaa cgcgccaggc
    30601 gcgccgcgct cggcccgatc cacctcggtg ccgtgcaggt aactcaagag caggacgcgc
    30661 accgtcccgg tttggaagcc ggtgtagagg gtctcgcccc agaccgtgga gagcgcgccc
    30721 gagtaactgc cgccgtcgaa ccgggtcagg cagatgtccg cgccgccctg gttggtctgc
    30781 ccgccgaacg agccctcggt ggtgccggcg acccaggcgg ttccgtcgcc ggcgaccgcg
    30841 acgccgcagg cctcgtcggt ctcctccgag ccccacagcc gggtccactc gggatagccg
    30901 aaggaatcga accgggtcag gcacaggtcg cggacgccga ggaattccgc gccgccgatg
    30961 gacgcctccg tgtacccgca gaccagggcg c
//
LOCUS       DAOOTK010000027        29440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1136_length_29440_cov_4.210005, whole genome shotgun sequence.
ACCESSION   DAOOTK010000027 DAOOTK010000000
VERSION     DAOOTK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 29440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29440
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_29440_cov_4.210005"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PK634_05010"
     CDS             complement(<1..499)
                     /locus_tag="PK634_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97128.1"
                     /translation="MKPDTPVKVGVRLSSRAVLARGAAAWLLASALGMPGGMAFAATV
                     TYTQQQNQYDTRWDSGGGTYDTGGSTELGMWAGGDQGYTVAWKTFRTASATSSSAREL
                     QVGDEFTIRVYTYGCYYGEIGVSLNDGGSTGAPWANRVSGSRMSVRQDGGNYGSGGGI
                     GSWYAI"
     gene            complement(791..3034)
                     /locus_tag="PK634_05015"
     CDS             complement(791..3034)
                     /locus_tag="PK634_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97129.1"
                     /translation="MNLPGKEPGPSGTWPWAAALLALAFAAYLPVLGAGFVWDDDNHV
                     TENATLTSAQGLRGIWLGLRENETCQYYPLTFTAFWAQYRLWGLNPLPYHLVNVLLHG
                     LNAILLWRLLARWRLPGAWAAAAIFAVHPIHAMSVAWVTELKNVLAGAFMLLTLLAWE
                     AWDRRRTAGLYALALLAYLAAVLSKTAAAALPVLMLLVAWWRKPRLEGRDARAVVPFL
                     LLGLAMGAVTMWVEQRLVWGRGPGLELDYAQRAFLACRSFWFYAAKILWPAGLNFLYP
                     KWTLDPGRAVAWLAPLALAALLAGLVAARRRLGRGPLVVALYFFLAAPALVLLHVLYM
                     MRYTYVSDHWIYFGSPALIALVTAGAARALKDWRQEAAGLLVLVLALLGVLTWMRASA
                     FRDEETLWRTTLARHPGAWLAANNLGILLGREGRYAEARVLFEDARRLEPAYAETLVN
                     LGNIDDALRGPDAALAWFEKALAVDSNNPIAHFNAGFALERLGRREEALAHYRRTLEE
                     RPNTGPAHYNMGNTLARLGRLDEAEVHYRSAIEIDPLDALALSNLGSVLTRAGRHAEA
                     LEILRRAAVLRPDDGQVMFHLGQTLLRLQRTDEAVAALLASARALPDRPDAPRELALA
                     WRARGDRARAESYLRESLRRDPEYRPAQNDLAWLLATGPDGNSARRQEALDLAERLYA
                     SGDPGPPDYLDTLAASYAGMGRFAEAKATALRALDLAQQDGNKEVANALRQRIEQYEK
                     GQPWVEP"
     gene            complement(3031..5553)
                     /locus_tag="PK634_05020"
     CDS             complement(3031..5553)
                     /locus_tag="PK634_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97130.1"
                     /translation="MPKASRGLGLAALALGLAFVVRAAYLYAQSDAPAFRHPEVDAAY
                     HDYWARAMVSGDWTPPPGRPDPEIQPRAYFRPPGYPFFLAAVYRVLGPDYLLPRLAQA
                     ALGLLNVWLAFVAARRWFGAWAGGLAAIFMALYAGFVYFEGELLEPVLLVTLSLGFML
                     ALGPGRAGRPRLRAAAAGALLGLYALVRPNGLLFAPAAIGWLAWRALRAGTPRAWRAP
                     ALALLAGLGVAVAPAAIRNLRAAGDAVLISSNAGINLFIGNHPQAEGVVMFTLPGLGV
                     FGTSFDYPALARAVEARVGHPLKDSGVSRYFTGEALRFIRRDPAGFLRGLARKTLLFW
                     GPVEMGHNKEDYFELKRGPVLRWLPGSFALALALCGMGVFSLRRGAGYPAAAREAAAL
                     MGLYVAAIFLSHLPFFVAGRYRLPVVPFLLILGAAGVAEAVRAWREGRRGAALRACLV
                     GAAVLLPASINWAGYRPNEAKWRYEQFIALQAAGRAEEAQAAFAKLAAREPGFLHALY
                     HQMGTGLADAGRFEEARDFLADALRFKPGVPDTLASLGSVLYRLGRTEEARSYFAEAL
                     RVAPAHRAARLNLAALQAGEGRTEEAIVQYRELLVAAPDDPEVLGPLAAALEQTGRRE
                     EALPFQEQAVRAAPGDAQGHSSLGALYGESGRPAEALREFEAARRIEPGHWPAWFGAG
                     VVLARQGAWEECIRMMTQAARLKPDDRQPYFYMAGALLRLNRPDEALAHYQEALGAYS
                     NYVPWLAEAAWMLATHPLSREPQIAEARRFAGTAAQLTGYRDAQVLDALSAALARDGE
                     FKEAAARAEAGAALAAEAGRTNLAAALRNRAALYKKDKAYLE"
     gene            complement(5564..7825)
                     /locus_tag="PK634_05025"
     CDS             complement(5564..7825)
                     /locus_tag="PK634_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97131.1"
                     /translation="MKDAASSEKQTILLTAALLAAAVFIAYTPVLNAGFIWDDNEFVT
                     ENPDLDTLAGLKTIWFHPKDNPHYFPLLMTVFWVLRQVLGVHPMGYHLVTLAFHIANV
                     LLVWLILRRLGERSAVLAALVFGLHPVHVQSVAWVTELKNTLSGFFFLGALYAWLDAD
                     GPGPAGGRRGWGGYAACLGLFLAALLSKTTVVSLPLAIVLVDAWRGGRLRRRTLVLAA
                     ALAALALAPAWVTMGLERSRNVEGLSAVFTTGERWLVAGRSFWFYLDKLFRPSGLMMI
                     YPPWPMDAARAAQYAPLLLAFLAVAGLWAGRRAWGRAPGLGLLFFAAAVMPIPFLDIN
                     FVLQHAFVADHFLYLPSLGILPVAAAGLYRLAERLKRPGAAFAVPLVLGAMTWAHARV
                     FRDPGTLWRDVLARNPYAPAAHNNLGLHLSKQGDPVGAIEQFRQAIRLHPGSSKAWNN
                     MATELLRIGEEEQAFAALRKAVELNPAYFEARNNLAALLTKRGALDEALIHLRRALEL
                     KPDYFEGYYNLGSLLAGAGRPAEALPPMRRALAMRPQDAGVVNNLSRAISEGLPPAVA
                     AAELRALAASYPGHLPLRVRLAEALWRAGDRAASEAEFEAILRQFPGSVEALLQLALA
                     RQRAGADATARDLLEQAVAAEPDHAGAIHALAWRLATHPDAGQRDGARAVTLARQACE
                     RLSYGDYTLLDALAAALAETGQFEEARRIVLLAVRQAPPDERRGAIEARGRLYEQELP
                     YRDPSFAGVTHDE"
     gene            complement(7882..9414)
                     /locus_tag="PK634_05030"
     CDS             complement(7882..9414)
                     /locus_tag="PK634_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97132.1"
                     /translation="MNLRSGAAWGLAAWAAAGGLAVLTAATRLPYSYDEVSAAHCVWL
                     VSGGATPYADFAVNHPPFYWYLLAPWSRWLPPDARALAVYRAVAIAGRGLFLAFLAGL
                     ACRGLDRGARPWAALGIAVVAVSAPALDYLVKFSPDAISNALLFGALLFLARPGVRPA
                     AAWFAGGFVILVSLLSNIKYGVFALLIGPVALAADLRRGGRPWKPAFWLLAGTAAALV
                     FACASLRFAGIEPGWAWDMVFRYNAFRERLAMARGGLRLELLRHPLLLLYAGAGLAAW
                     LADAARARRLEPLLLAALGFLAASAATVSRPYSQYLGTWFLLAALFPARAWAPRLARG
                     RGAWRALVLLLALAVPLQAAWHYSRPGLRRAREEQQAVFRFMEAATPRDGFVIAHMAY
                     HPVCRSNTFYKFSADMDGREDAYDRVAAAIPGFRPADRFTPAQYARDLERHPPAVIFE
                     GFVRAGRLEGGPYMTAAQAEALGRFLQERAAEYEYLEVSSPRHPEWPPFVVTRRRTPP
                     PP"
     gene            complement(9411..11180)
                     /locus_tag="PK634_05035"
     CDS             complement(9411..11180)
                     /locus_tag="PK634_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97133.1"
                     /translation="MTMVAGAAALLFFINLWAAAAFWAGCPLPFDAAAVLGMASALIV
                     VGWRRKQVAEGKASGRAVRLAAFLLLSLPLYLWIRTHVGHDWLCHIPLTGSIGRGNLP
                     VRDLASPGRLLPYHYGFDVLAGAFLRLARLAAPGRHVESALDATSWAALVLLLAAAWA
                     WWRGFNAGLPEEWRVGRGEMILAMILMLCGGGLVFLGKFLDPSVETWRNNVYAACHPL
                     PLFIGRRPTPLGQAFFLFQMAALPGLRDPARRRPAAWASLAVSNAALWLTSEDLAVAS
                     ALVALGLLLSPAWRAAARFFLGAAAAALAVAAIQGGLLTALVFHPGGAGVAGWRIRPP
                     FMPGWGLGEPGWGQGRFWAAWLAEFPVALWLLPAAGLAARFQKKRLPPEWKALAACAG
                     LFLLLPLFVQARFSPNDLHRLFFLPQMISLLMLPLHLRILLPRPAWRTPILALVAALL
                     VAGPLPALRAEVRRFDALYGPGWQPDLALPLPADRFERGAVWLVSTRYLPALHYNGLF
                     ALSAPFGMYGPNYFKIYPAQHEEWLARCLEDPVRFGATRALLSREDAARLRANGAPFR
                     AEDVIDSAGEPAVLAFFSNGSGP"
     gene            complement(11191..12726)
                     /locus_tag="PK634_05040"
     CDS             complement(11191..12726)
                     /locus_tag="PK634_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97134.1"
                     /translation="MISRGGFLVVAALLAAAAWPASRTRVVTYDEASHAHMAWLLDRG
                     QVPYRDFAANHLPFFWMLVRPLASRLADPLRTMNLARGLALAGHLAFLLLLAAHAARG
                     RDSGARACALGALAFMALLPPVLPFLVEFRPDSLSNPLLAGGLWLLRGRPSRRRAAAG
                     GFLVVAAMLINTKLLLLPVVLAAAALWRADDRRAAWARAGWAAGGAGLALLLAAGVLK
                     GYGIDPRAAWQSAVAYNAALERMHTFGAGLWRALRDQPLPLACAAAGAAGWGWRVARR
                     RERAGAFETGVVLFLLLQAVLATKPWLQYTASWFLLAAVLPAALAARAAEEWPRWWLG
                     PAALVLVLLALRALTAPAAEQGPVIRFADGSRFSPTRTVQARALRYLLDRAAPHDRVV
                     AAFPWHPLCRSNTFYKIISDFDGRGHDGLEWAARNLPGFPRADRLTPEGYRSELEERP
                     PAVVFLGILSEGGPAWSPYSEAQTRALEAFFDAHGAEYGMAEIPGTPFTVMQRVDNTR
                     PAP"
     gene            12852..13274
                     /locus_tag="PK634_05045"
     CDS             12852..13274
                     /locus_tag="PK634_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97135.1"
                     /translation="MSTWTRSGLWFLAAAVALSLATPSLAGWPAPDQMKDIRSIAEQL
                     VKWSNKLKEEYQGKWPGDQDFLNRAGRFCNRCQHLVNIVNVAIDKGEGGRHTQSAYGE
                     VREAWRAVYTASGAGHTDDWKERKEINKLMDRLAPYYQ"
     gene            complement(13350..14291)
                     /locus_tag="PK634_05050"
     CDS             complement(13350..14291)
                     /locus_tag="PK634_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97136.1"
                     /translation="MNKDNDSLITRRALKDLPGVLVLALLCAAGPAFGQILPGTLGVD
                     TTTTLNENNVVLEDGDLVQILWATSNIPYPPDYDGNPDPRTAPMADIAEPSGITNACV
                     RIGDGLVPGAPGYEPGSIVVLVSNTVRDADAYLGTPVFVRVFNASTATGASFYGEAAP
                     QPVPEDGHLYFNIDKTRYALDTRDNDGDGLNNSWEESGGTDPDNPHSDTDGVNDWEEW
                     VAGTDGRDPDSVFILARIQQGAGDEAVVTWDSVAGKRYEVYYTTDPLDAEDPPPTWSK
                     VSGPDPITATGALTELPVSSGLAAGRGHFRVVVLQDE"
     gene            complement(14288..14923)
                     /locus_tag="PK634_05055"
     CDS             complement(14288..14923)
                     /locus_tag="PK634_05055"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW97137.1"
                     /translation="MLGLAIGIVCLTSVPARGDIISLIAYVSSALLLDDGITALPYGS
                     IVYIYGDANGDIDPMAMFNDCYLPGTATDDDILLGVVHIGQPYYNAGGGDIDGTFLSD
                     MVITWDNTEVNIENLYIRFFNTLDFTGGEVAWGQSPLYEAEENFLVVEQDFEGGWEAD
                     SNNCFVVIPEPSTAHLMLLSLALLAGMGASVRKQSRSQRADEKASKEASAS"
     gene            15229..16041
                     /locus_tag="PK634_05060"
     CDS             15229..16041
                     /locus_tag="PK634_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOW97138.1"
                     /translation="MSQLPPSGPRRASRPGGRAAGTIRALILAGQAAWALGAAAAIHE
                     SDHTFWLTIETAPPGATVLAVPGDGREKEQALGVTPCALAVDLNWEVRWFKKRWESLT
                     ILSPGGFCRPTFNTNEGYRLTARLVVRKEGWKDIPIDTHLATLADPGKDWSGKEQWPA
                     KQTLRFVLEALPPASPPSSAAARAAPRRVVLAGAGEGSGKVETGRVTIFCETASAEVW
                     VDGQSAGSAPVELLLRAGKRLVEVRAGDRLVYRREIELEPDASLSFIVPPGE"
     gene            complement(16055..17056)
                     /locus_tag="PK634_05065"
     CDS             complement(16055..17056)
                     /locus_tag="PK634_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOW97139.1"
                     /translation="MKGISHFVSGVMAASFCPWAVRAAAEGNPLYFILGGAFGILPDT
                     IDFKFYRFFYRHDFYVDPDPQNPDPQAIADELARAVDKARTAGRAVRIKLNTVRMGAD
                     YWRQYIVRFDAENQEVQVRIGPVVNTGQVPVPNSEPAERKIGRARLAAPIVQTYDATT
                     RVDIFDGPTFALEADGQGRVVLHFLPWHRNWSHSFFVGAAWALLGWLLWSWQAAVVIM
                     AGYAAHLLEDQLGFMGSNLFFPITKRRFIGLHVMRSGDAVPNFSAVWLSCLLIFWNLY
                     RMTPDLLYHFNFLQLIFFGAVLPIGLFGLVHWLLTRGDKGREGPVELEDEFGDTMTS"
     gene            complement(17070..18407)
                     /locus_tag="PK634_05070"
     CDS             complement(17070..18407)
                     /locus_tag="PK634_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOW97140.1"
                     /translation="MMLNILTVLGVTILGTVLSCTISVLPALHAYNVLGALVLLFYWL
                     IEKGMSIPSEVFLPFMAGIVVGWSMLNTIPSILLGAPDESAMFTVLPGQKYLMSGRGY
                     EGTMMTGVGGLAGVFFLVCVVGPFAPRVLPVVQGVLRPHMHWILWVIITYILMSEWPK
                     GGNRGPAGLAKFADAWRSLGAGLATFFLSGLLGFILLYRSPVSVDMAFQNIMPAFVGL
                     FAVPWCLLNMISGANVPPQRIAHSLELNGDLILRSAAAGGLGGGFAAFFPVITGGIGG
                     LLAGHATAQRDEKVFIMSQGVSKLVYYVGSFMLLFVPGLGLTRGGAAWMMKGLYVPRS
                     YYDYFMVLGAIAISGAVAFLMMGPLTRATIRFIEKVHYRNASLVALVIILAIVYLMTG
                     WGGILVMIVATGIGLIPVLFASRRLNCLGILLLPIACNMSGFGQQIAMWLRLI"
     gene            complement(18423..19961)
                     /locus_tag="PK634_05075"
     CDS             complement(18423..19961)
                     /locus_tag="PK634_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97141.1"
                     /translation="MTTNPLPGHADQPAEITCAQCGQFVGALTRCPRCGARVEKRMSI
                     RVFRYAALALGTIGLGLLYLMATHRELPVVRIGEIKPTMNFAYVRVVGAVSGEPRVFK
                     EAGKVRSLRFSVDDGTGEISVTAFRSQAEALVEQDLVPSPGDRVTVAGALSMTADDDV
                     VMRLQVTEHLQLERAEVPVTVLGEVNGELVGNSILVEGTITAVNPPKPESRQPWVIEL
                     RDDSGEGAITFWPDLYPDIGDKSLLIPGAAVRARVGVRTYKDKLQLMLNRGEDLEVRA
                     DSAERQVAGPGAPAEEKPAPAAAAPRSVPTRVEAATEIADVTVDMKGKEITVLGRVAE
                     LRAPAPGSRAPYTMVLEDQGRRIPVVYWDDVATRLGENAPSTGVLMKVRGLVDVYEKT
                     VQIKVTHSAKVKLVDVEPPAAPAPAGEGLVEIGKLTADMKGRVFTVRGALGEPRSLMK
                     GIVYPLSDGTGQIDLLLWDRMVPGPERDKLEPGRTVKATGAIGDYQGQLQIVPASAQA
                     VELE"
     gene            20096..21565
                     /locus_tag="PK634_05080"
     CDS             20096..21565
                     /locus_tag="PK634_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW97142.1"
                     /translation="MIRASRQRADNERARRRAEQALAESAREYPLLAESLPDALFITD
                     RSGLVCFVNSAACRFFRRPVSALVGKRQTDLFPQPVARQHTRAIQKVCRTGQPHIKME
                     PERLPGTDLWIETRLQPIRNRRGRITHVLGLARDISARVKAESALRESEENFRAMAEN
                     GIDAILIAVPPGHQVFVNRRLAELTGYSIRELHRMHISRLARPPEARRVLDMFRRRLR
                     GEEAPQRYEAMILRKDGRETPVEVSATRTLWQGRAAVMAIVRDVAVQRRLAEERRRLA
                     SRLIEIQERERRLISSALHDHLGQILTLARLEMGAVQPARRDSILHRQKALLRLDEAL
                     ATVRNLATSLRPPILDDLDLETALEDLAGQFGDSGIDIRFSHRGRPSPQSRQIKTCLY
                     RVTQEALTNVVRHAQATRVRIHLDVRAGTTRLTIRDDGKGFDQAAIPESQGIGLAGMR
                     ERLRLCRGRLEIRSVKGRGTGVVAVIPPPGSRVILEDIR"
     gene            21562..22194
                     /locus_tag="PK634_05085"
     CDS             21562..22194
                     /locus_tag="PK634_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW97143.1"
                     /translation="MIRLMLADDHVMLREGLRSQLKQSPDLQVVGEAGTPAEVMEQLP
                     SLKPDVLILDLNFPDGNGITLLGQIGQAWPSCRVVVLTMYNHVRYAQHAMESGARGFV
                     VKGAPFEELVEAIRAVHRGETYMCRTMRGRLAAQKRRGRRPDALGSLSQREFEVLVLV
                     GNGLAMKEIAAQLGVSEKSVTTYRARIQQKLQMRNKAELIRFALENGLVE"
     gene            22438..22998
                     /locus_tag="PK634_05090"
     CDS             22438..22998
                     /locus_tag="PK634_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOW97144.1"
                     /translation="MELHPTMWRTCRVLAGPTRLRLLRQIIAEPGLPVSGLADRAALK
                     LSRASQELRRLSSRELVQAVRQGREVRYRPYPDPQVPSASPLLAALTAALRRGDAMAA
                     DCRRVAAAFSHPRRIAIVRELLRGPRPAPELAQALPMHSRTLYRHLRWLKHTGTVKEE
                     GARFLFVPDANPLLRCIVRLLHRQRE"
     gene            23133..24632
                     /locus_tag="PK634_05095"
     CDS             23133..24632
                     /locus_tag="PK634_05095"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOW97145.1"
                     /translation="MADTVSIRSLQERLEQVVSIRGWLYGKRSGGRIAFLLVRDGTGL
                     CQCVVEAAQAELFSAAESLGQESSLMVTGRVRADSRSPGGAELAVTGLQVLQSASDYP
                     ISRKAHGTDFLMEHRHLWLRSPRQAAILRVRHTLIRAARRYFDDHGFTLVDTPILSPG
                     AAEGAGTLFPVDYFGDTVYLAQTGQLYLEAAAMALGRVYCFGPTFRAEKSKTRRHLAE
                     FWMIEPEIAFAELPELMTWAEGLVCAMVGAALSERREELALLGRNPAALERIAPPFPR
                     LTYSQAVDLLRDPALHRKLEQELEADRARLKTLIPELAALDEQLSRARQGWQQDKLAQ
                     QAADLRERIRDLEAQVAQRPIHIEHARNFAWGEDFGGDEETIISRQFDRPVLITEYPR
                     RIKAFYMKPSPANPDVVLNLDMLAPEGYGEIIGGSQREDDLAALRQRMAEEGMNPAPY
                     EWYLDLRRYGSVPHGGFGLGLERTVAWICGLKHIRETIPFPRLMGRIYP"
     gene            24629..25138
                     /locus_tag="PK634_05100"
     CDS             24629..25138
                     /locus_tag="PK634_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOW97146.1"
                     /translation="MSTRPSRPLKIGVLGPHKATDDELQLGREVGAEIARRGATLLCG
                     GLDGVMAAASEGAKTAGGLTIGILPGDTADGANPFIDIAIPTGLGAYRNMLLVRACDA
                     VIAVRGEYGTLSEIAAALRLKVPVVGLQTWKLVRNGIEDPGLRVAATPSEAVDLALRL
                     AASPPTART"
     gene            complement(25122..25985)
                     /locus_tag="PK634_05105"
     CDS             complement(25122..25985)
                     /locus_tag="PK634_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97147.1"
                     /translation="MRLGRAVLAAAWLVAASRAPAAPPADPNWQEVARGQVDTKALTP
                     RGQTALDMPGLKWRHAHTPHFVVHFEQEIFARKVGRMAEFFYGYIGADLEGATDRLEG
                     RSHIFIFRTQKDWKAFGASAGDVPEWSFSQVAGPVMFLQQAADTSSSGDVLAHEMAHL
                     VVNRFLERPPPLWLNEGLAEFYEEFAYSAFKGIKKSRRSQFRRLNRVFPVASLLEASA
                     YPNDTASVHAFYETSKYLVGWLRLDRPADRFVPFVADLAAGQGYQAVFAARYGLNSVA
                     AIEAEFLKFAR"
     gene            complement(25991..26470)
                     /locus_tag="PK634_05110"
     CDS             complement(25991..26470)
                     /locus_tag="PK634_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOW97148.1"
                     /translation="MSTKLTADEILRMAQRLEEKGARYYRKAAKLHVAGRELLNRIAG
                     QEDQHYQFFEDMRRSLTPAEKPEGEDPYGDLAPYLDAMVDHLKIQINQSPEDALTGRE
                     PLADILRAAIQMEKDAVLFYSGLRDAAAPALGRKRLDDIIHEEMRHIVWLSAQAKAS"
     gene            complement(26496..26996)
                     /locus_tag="PK634_05115"
     CDS             complement(26496..26996)
                     /locus_tag="PK634_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOW97149.1"
                     /translation="MPITFNANEALKVAERIEGNAGAFYRKAAKLHSGERSKDLLFKL
                     AAMEDQHQETFSLMRTQLAAAEKEPTTYDPMDEELLYLKALADQHGGEGSPTAADALT
                     GKESMEQVLRVAIDLEHKTVQFYTGLRDRVPASLGRDKLDKIIAEEKSHVVQLSRELH
                     AARGKA"
     gene            complement(27073..28107)
                     /gene="hypE"
                     /locus_tag="PK634_05120"
     CDS             complement(27073..28107)
                     /gene="hypE"
                     /locus_tag="PK634_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOW97150.1"
                     /translation="MTFPTIGKSASERILLSHGGGGGRTKQLLRDVILPALGYDGSEK
                     LDDAAYVETPDREVAVTTDSYVVRPLFFPGGDIGRLAVSGTVNDLAMQGAAPLALTLG
                     LVIEEGLEIDVLRRAMESVGRTAREAGVRIVTGDTKVIERRTGDGLFINTAGLGSRYR
                     GADTGVGNARPGDAVLVSGTLGDHGVAVMSAREAWGLSSRLESDVAPLWDMVRGLLDA
                     VPGVRCLRDPTRGGLAAALNDIAEASGAGVRIREDALPVRDEVRGVCDLLGLDPLNVA
                     NEGKTVVVCAAADAERALAALRAHPRGRAAAAIGEVVKGPAGRVRLATRLGGERMVDA
                     PSGEDLPRIC"
     gene            complement(28203..29267)
                     /gene="hypD"
                     /locus_tag="PK634_05125"
     CDS             complement(28203..29267)
                     /gene="hypD"
                     /locus_tag="PK634_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOW97151.1"
                     /translation="MSAGESIRAIQALAHSLGRAVQLMEVCGTHTMAAFRTGLRSLLP
                     DNVKLLSGPGCPVCVTPIDYVDKAVALAGRPDTVVATFGDMIRVPGSRESLEQARARG
                     ARVRVVYSPLDALAEARANPGTAVVFLGVGFETTAPTTAWALREAARDTPNFFVLCGH
                     KTMPRAMAALLAGGEVRVDAFLCPGHVSVIIGSAPYEFIPRQYGRPCVVAGFEGEDLA
                     AAIRMALRQLADGRAEVEVQYTRSVSREGNAKAQAAIREAFEECDAAWRGLGVIPGSG
                     LRIRPEWAAHDAERLLAGLDRPPAREPKGCRCGDVLRGLCAPPECPLFRKVCTADQPV
                     GACMVSSEGTCAAYYRFGKT"
BASE COUNT         4751 a        10664 c         9941 g         4084 t
ORIGIN      
        1 cgatcgcgta ccacgacccg atgcccccgc ccgacccgta gttcccgccg tcctgccgaa
       61 cggacattcg cgacccgctg acccggttcg cccacggcgc gcccgtcgag ccgccgtcgt
      121 tcagcgaaac gccgatctcg ccgtaatagc agccgtacgt gtacacccgg atggtgaact
      181 cgtcgcccac ctgcagctcg cgcgcggagg aggacgtcgc gctggccgtg cggaacgtct
      241 tccaagcgac cgtgtagccc tggtcgccgc cggcccacat gcccagctcc gtgctgccgc
      301 ccgtgtcgta cgtgccgccg ccgctgtccc accgcgtgtc atactggttc tgctgctgcg
      361 tgtaggtcac cgtggcggcg aaggccatcc ctcccggcat cccgagcgcc gacgccagca
      421 gccatgctgc cgccccgcgc gccaacaccg cgcgggaaga caaccgtact ccaaccttca
      481 cgggtgtgtc aggtttcatg atcggcctgc ctcaatccgg tcaacccgga acactggatt
      541 attagcaagc ctttccgccc ctccagcgca agcggaaggc tcgtcgtcac gcctccggca
      601 aaaccccttt tctgaaacgc ccttcctgat gaaaagtata gggcgtttgg caattcatgt
      661 caaacagcac cccccccgca tccggcccgc cgaatgctgc ctttccaaag ctacagtcgc
      721 ttgagagagg ccgactgtcc tttccttttt ttaaggagga atactccgca accgatgccg
      781 ggaagacgct ctacggctcg acccagggct gccccttttc gtattgctcg atcctctgtc
      841 gcagcgcatt cgcaacctct ttattcccat cttgttgcgc caagtccaat gcgcggaggg
      901 cggtggcctt cgcttcggca aaccggccca tgccggcgta cgacgccgcc agcgtgtcaa
      961 gataatccgg cgggcccggg tcgccggaag cgtacaagcg ttccgccagg tccagcgcct
     1021 cctgccggcg cgccgagttc ccgtccggcc ccgtagcgag cagccaggcc agatcgttct
     1081 gcgcgggccg gtactccggg tcccggcgca gggattcccg gaggtacgac tcggcccggg
     1141 cccggtcgcc gcgcgcccgc caggcgagcg ccagttcgcg gggcgcgtca ggccggtccg
     1201 gaagggcgcg cgccgaggcc aggagcgcgg cgacggcttc atcggtccgc tgaagccgca
     1261 gcagggtctg gcccaggtgg aacatcacct gaccgtcgtc gggccgcagg accgccgcgc
     1321 ggcgaaggat ctcgagcgcc tccgcgtgac gcccggcccg ggtcagcacg ctgcccaggt
     1381 tggacagggc cagggcgtcg agcggatcga tttcgatcgc ggaccggtaa tggacctccg
     1441 cctcgtccag ccgtcccagc cgggccaggg tgttgcccat gttgtagtgc gccgggcccg
     1501 tgttcggacg ctcctccagg gtgcgacggt agtgcgccag ggcttcctcg cggcggccca
     1561 gccgctccag cgcaaacccg gcgttgaaat gggcgatggg attgttcgag tccacggcca
     1621 gcgccttctc gaaccacgcg agggccgcgt ccgggccgcg cagggcgtcg tcgatgttgc
     1681 ccaggttgac cagggtctcg gcgtaggccg gctcgaggcg ccgcgcgtcc tcgaacaaca
     1741 cgcgcgcctc cgcgtaccgg ccctcgcgcc cgagcaggat tcccaggttg ttcgccgcca
     1801 gccacgcgcc gggatggcgg gccaacgtcg tgcgccagag ggtttcctcg tcccggaacg
     1861 ccgaggcccg catccaggtc aggacgccca gcagcgccag gacaaggacc agcagtccgg
     1921 ccgcctcctg gcgccagtcc ttcagggcgc gggcggcgcc cgccgtgacg agcgcgatca
     1981 gcgccggcga cccgaaatag atccagtgat ccgacacgta ggtgtaccgc atcatataaa
     2041 gcacgtgcag cagcaccagc gcgggcgcgg ccaggaagaa gtagagggcc acgaccagcg
     2101 ggccccggcc cagccggcgg cgcgccgcaa cgagcccggc cagcagagcg gccagcgcaa
     2161 gcggcgccag ccaggcgacg gcgcggcccg gatcgagggt ccacttcggg tacaggaaat
     2221 tcagcccggc cggccacagg attttcgccg cgtagaacca gaacgagcgg cacgccagga
     2281 acgcgcgctg cgcatagtcc agttcgaggc ccggcccgcg cccccacacc agccgctgct
     2341 ccacccacat ggtcaccgcg cccatcgcca ggccaaggag caggaacgga acgaccgccc
     2401 gcgcgtcccg tccttcgagg cggggcttcc gccaccacgc aaccagcagc atcagcaccg
     2461 gcagcgccgc cgccgccgtc ttgctcaaca ccgccgccag gtaggccagc agcgccagcg
     2521 cgtacaggcc ggccgtcctg cgccggtccc acgcctccca cgccagcagc gtcagcagca
     2581 tgaacgcgcc cgccagcacg ttcttgagct ccgtgaccca cgccacggac atcgcgtgga
     2641 tcgggtgcac ggcaaagatc gccgcggccg cccaggcgcc gggcagccgc cagcgggcga
     2701 gcagccgcca gagcaggatg gcgttgaggc cgtgcagcag cacgttgacc aggtgatagg
     2761 gcagcgggtt cagcccccag agccggtact gcgcccagaa cgccgtgaag gtcagcgggt
     2821 agtactggca ggtctcattt tcccgcaggc cgagccagat gccccgcaac ccctgcgccg
     2881 aggtcagcgt cgcgttttcg gtcacgtggt tgtcgtcgtc ccacacgaag ccggccccga
     2941 ggacgggcag gtacgccgcg aacgccagcg cgagcagcgc cgccgcccac ggccaggtcc
     3001 cggacgggcc gggctctttt ccgggcaggt tcattccagg tacgccttat ccttcttata
     3061 gagcgccgcc cggttccgca aggccgccgc gaggttggtc cggcccgcct ccgcggcaag
     3121 ggccgcgccc gcctccgcgc gggcggcggc ctccttgaac tcgccatccc gggccagcgc
     3181 cgcggaaagg gcgtcgagca cctgcgcgtc gcggtagccg gtgagctgcg ccgccgtccc
     3241 ggcgaaacgc cgggcttccg cgatctgcgg ctcgcgcgag agcgggtgcg tggccagcat
     3301 ccaggccgcc tcggcaagcc acggcacgta gttggaatac gccccgaggg cctcctggta
     3361 atgggccagg gcctcgtccg ggcggttgag ccggagcagc gcgcccgcca tgtagaaata
     3421 gggctgccgg tcgtccggct tcaggcgcgc ggcctgcgtc atcatccgga tacattcctc
     3481 ccacgcgccc tgccgggcga ggaccacgcc cgcgccgaac caggccggcc agtgtcccgg
     3541 ctcgatccgg cgcgccgcct cgaattcgcg cagggcctcg gccggccggc ccgattcccc
     3601 gtacagcgcg ccgaggctgc tgtgcccctg cgcgtcgccg ggcgccgcgc gtacggcctg
     3661 ctcctggaac ggcagggcct cctcccggcg gccggtctgc tcgagggcgg cggccagcgg
     3721 gccgagcacc tccggatcgt ccggcgccgc caccaacagt tcgcggtact gcacgatggc
     3781 ctcttccgtc cgcccctccc cggcctggag cgcggccagg ttcagccggg ccgcccggtg
     3841 cgcgggcgcc acccgcaggg cctcggcaaa gtacgagcgc gcttcttccg tccggcccag
     3901 ccgatacagc acggaaccga ggctcgcgag ggtgtcgggc acgcccggct tgaaccggag
     3961 cgcgtccgcc aggaaatccc gcgcctcctc gaaccgcccc gcgtcggcca ggcccgtgcc
     4021 catctggtga tacagcgcgt gcaggaagcc gggctccctg gcggccagct tcgcgaaagc
     4081 cgcctgcgcc tcctccgcgc gccccgcggc ctgcagcgcg atgaactgct cgtaccgcca
     4141 cttggcctcg ttgggccggt agccggccca gttgatcgag gccggcagga gcaccgccgc
     4201 gccgacgagg caggcccgca gcgcggcgcc gcgccgcccc tcgcgccacg cccggaccgc
     4261 ctccgccacg ccggccgcgc ccaggatcag gaggaagggc acgacgggca ggcggtaacg
     4321 gcccgcgacg aagaacggga gatgactcaa gaatatggcc gccacgtaca gccccatcag
     4381 cgccgccgcc tcgcgggccg cggcggggta ccccgcgccc cggcgcaagg aaaagacccc
     4441 catgccgcag agcgccagcg ccagcgcgaa ggagcccggc agccaccgca gcaccggccc
     4501 gcgcttcaac tcgaagtagt cctccttgtt atgccccatt tccaccgggc cccagaacag
     4561 cagcgtcttc cgggccagcc cgcggaggaa acccgccggg tcccggcgga tgaaccgcag
     4621 cgcctcgccc gtgaagtagc gcgagacgcc ggaatccttg aggggatgcc cgacccgcgc
     4681 ctcgacggcg cgcgcaaggg ccgggtagtc gaacgacgtg ccgaacaccc ccagccccgg
     4741 cagggtgaac atcaccacgc cctcggcttg cggatgattg ccgatgaaga gattgatgcc
     4801 cgcgttggag gagatcagca ccgcgtcgcc ggcggcccgc aggttgcgga tggccgcggg
     4861 cgccacggca acgccgagcc ccgcgagcag cgccagcgcc ggcgcccgcc aggcccgggg
     4921 cgtcccggcg cggagcgccc gccaggccag ccagccgatc gcggccggcg cgaagagcag
     4981 gccgttgggc cgcacgaggg cgtacaggcc gagcagcgcc cccgcggcgg ccgcgcgcag
     5041 gcgtggccgc cccgcccgcc cgggccccag cgccagcatg aaccccaggc tcaacgtcac
     5101 cagcagcacc ggctccagca attcgccctc gaagtataca aagccggcgt acaacgccat
     5161 gaagatcgcg gccaacccgc ccgcccacgc gccgaaccag cgtcgagcgg cgacgaaggc
     5221 cagccagacg ttcaacaggc ccagcgccgc ctgcgccagc cggggaagca ggtagtccgg
     5281 gcccagcacg cggtacaccg ccgccaggaa gaagggatag cccggcggac ggaagtaggc
     5341 tctcggctgg atctccgggt cgggccggcc cggcggcggg gtccagtctc cggacaccat
     5401 cgcgcgcgcc cagtagtcat ggtaggccgc gtccacctcg ggatggcgga aggccggcgc
     5461 gtccgactgg gcatacaggt acgcggcgcg cacgacgaag gccaggccca gcgccaacgc
     5521 cgcgaggccc agcccgcgcg atgccttcgg caagggcgtc cgattactca tcatgagtca
     5581 cccccgcgaa ggacggatcc cggtatggca actcctgctc gtagagccgg ccgcgcgcct
     5641 cgatcgcgcc gcgccgttcg tccggcggcg cctggcggac cgccaggagg acgatccgcc
     5701 gcgcctcctc gaattgcccg gtctcggcca gcgccgccgc cagggcgtcg agcagcgtat
     5761 agtcgccgta ggaaagccgc tcgcaggcct gccgtgccag ggtcacggcg cgcgcgccgt
     5821 cgcgctggcc cgcgtcgggg tgcgtggcca gccgccaggc cagggcgtgg atcgcgcccg
     5881 catggtccgg ttccgccgcc acggcctgct ccagcaggtc gcgcgcggtc gcgtccgccc
     5941 cggcgcgctg gcgggccagc gccagctgga gcagcgcctc caccgagccc gggaattgcc
     6001 ggagaatcgc ctcgaattcc gcttccgagg cggcccggtc tcccgcccgc cacagcgcct
     6061 cggccaggcg gacccgcagc gggagatgcc ccgggtagga cgccgccagc gcgcgcagtt
     6121 cggcggcggc cacggccggc ggcagtccct ccgaaatcgc gcgggacagg ttgttgacga
     6181 cgccggcgtc ctgcggccgc atggccaggg ctcggcgcat gggcgggagg gcttccgccg
     6241 gccggccggc gcccgcgagc agggaaccga gattgtagta gccctcgaag tagtccggct
     6301 tcagctccag cgcgcggcgc aggtgaatca gcgcctcgtc gagcgcgccg cgtttcgtga
     6361 gcagcgcggc caggttgttg cgcgcctcga aataggcggg gttgagttcg accgccttgc
     6421 gcagcgccgc gaaggcctgc tcctcctcgc cgatgcggag cagctccgtc gccatgttgt
     6481 tccaggcctt cgagctgccg ggatgcagcc ggatcgcctg ccggaactgc tcgatcgcgc
     6541 cgacgggatc gccctgcttc gacaggtgca gcccgaggtt gttgtgcgcg gcgggcgcgt
     6601 acggattgcg cgccaggacg tcgcgccaga gcgtgccggg atcccggaac acccgcgcgt
     6661 gcgcccaggt catcgccccg aggacgagcg ggaccgcgaa cgccgcgccc ggccgcttga
     6721 gccgttccgc cagccggtag aggccggccg cggcgacggg caggattccg aggctgggca
     6781 ggtacaggaa atggtcggcc acgaacgcgt gctggagcac gaagttgatg tcgaggaacg
     6841 ggatcggcat cacggcggcg gcgaagaaca ggaggcccag gccgggcgcc cgcccccacg
     6901 cgcggcggcc ggcccagagc ccggccacgg ccaggaacgc cagcagcagc ggcgcgtact
     6961 gcgcggcccg cgcggcgtcc atcggccacg gcgggtagat catcatcagg ccggacggcc
     7021 ggaacagctt gtccaggtag aaccagaacg agcggccggc gacgagccag cgttcgccgg
     7081 tcgtgaagac cgccgacagg ccctcgacgt tccgcgagcg ctcgagcccc atcgtcaccc
     7141 aggccggcgc cagggccagc gccgcgaggg ccgcggccag gacgagggtg cgccgccgca
     7201 ggcggccgcc gcgccaggca tccacgagga cgatcgcgag cggcagcgac accacggtgg
     7261 tcttgctcaa cagcgcggcc aggaacaggc cgaggcaggc cgcgtacccg ccccaccccc
     7321 gccgcccgcc cgccgggccc ggcccgtccg cgtccagcca ggcatacagc gcgccgagga
     7381 agaaaaaacc cgacagggtg ttcttcagtt ccgtcaccca ggcgacggac tggacgtgga
     7441 ccgggtgcag cccgaacacg agcgcggcca ggacggcgga ccgctcgccc aggcgacgca
     7501 ggatcagcca gacgagcagg acgttggcga tgtggaacgc cagcgtgacg aggtgataac
     7561 ccatcgggtg cacgcccagg acctggcgca gcacccagaa gacggtcatc aggaggggaa
     7621 agtagtgcgg attgtccttc gggtggaacc agatggtctt gagcccggcg agcgtatcga
     7681 ggtcggggtt ttcggtgacg aactcgttgt cgtcccagat gaagcccgcg ttcagcaccg
     7741 gcgtgtaagc gatgaagacg gccgccgcca gcagggcggc ggtcaggagg atggtctgct
     7801 tttcgctgga cgcggcatcc ttcatgaaag gccgtcgggc agcataccag agccgccccc
     7861 ttgttcaacg cgcgatgccg ctcaaggcgg cggcggagtc cgcctccgcg tgaccacgaa
     7921 cggcggccat tccgggtggc gcggcgagga gacctccagg tattcgtatt ccgcggcgcg
     7981 ctcctgcagg aaccggccca gcgcctccgc ctgcgccgcc gtcatgtagg gccccccctc
     8041 gagccggcca gctctgacga atccctcgaa gatcaccgcc ggcggatggc gttcgaggtc
     8101 gcgcgcgtac tgcgccgggg tgaagcggtc ggccgggcgg aagccgggga tggcggcggc
     8161 cacccggtcg tacgcgtcct cgcggccgtc catgtcggcc gagaacttgt agaacgtgtt
     8221 gctgcggcac accggatgat aggccatgtg cgcgatcacg aatccgtcgc ggggcgtcgc
     8281 cgcctccatg aaccggaaca cggcctgctg ttcctcccgc gcgcgccgga ggccgggccg
     8341 ggagtaatgc cacgccgcct gcagcggaac ggccagggcc agcagcagga ccagcgcccg
     8401 ccacgcgccg cgcccgcgcg ccagtcgggg cgcccaggcc cgcgccggga acagggccgc
     8461 cagcaggaac caggtgccga ggtactggct gtacggccgc gagacggtgg ccgcgcttgc
     8521 cgcgaggaaa cccagcgccg ccaggaggag cggctcgagc cggcgcgccc gcgccgcgtc
     8581 cgccagccac gcggccaggc cggcgcccgc gtagagcagc agcagcggat ggcgcagcag
     8641 ttccagccgc aggccgccgc gcgccatcgc cagccgttcg cggaacgcgt tgtaccggaa
     8701 aaccatgtcc caggcccagc ccggctcgat gcccgcgaac cggagcgacg cgcaggcgaa
     8761 gacgagcgcc gcggcggtcc cagccagcag ccagaaggcc ggcttccacg gacggccccc
     8821 gcgccggaga tccgccgcca gcgcgaccgg cccgatcaac agcgcgaaca cgccgtactt
     8881 gatgttgctc aacagcgaaa ccaggatgac gaagccgccg gcgaaccacg ccgccgccgg
     8941 ccggactccc ggccgcgcga ggaacagcag cgcaccgaag agcagcgcgt tggatatcgc
     9001 gtccggcgaa aacttgacca ggtagtccag cgccggcgcg ctgaccgcga cgaccgcgat
     9061 ccccagcgcc gcccagggcc gcgccccgcg gtccagcccg cggcaggcca ggcccgcgag
     9121 gaacgccagg aacaggccgc gccccgcgat cgccaccgcg cggtagacgg ccagcgcgcg
     9181 ggcatccggc ggcagccacc gcgaccacgg cgcgagcagg taccagtaaa acggcggatg
     9241 gttgaccgcg aagtccgcgt acggcgtcgc cccgccggac acgagccaga cacagtgcgc
     9301 cgccgacacc tcgtcgtacg agtacgggag cctcgtcgcc gccgtcagca cggccaggcc
     9361 gcccgccgcc gcccaggcgg ccagacccca cgcggcgccg gagcgaaggt tcatggcccg
     9421 gatccgttcg aaaagaatgc caggaccgcc ggctcgcccg ccgagtcgat cacgtcctcc
     9481 gcccggaacg gcgcgccgtt cgcgcgcagg cgcgcggcat cctcgcggga caggagggcg
     9541 cgcgtcgcgc cgaagcggac cgggtcttcc aggcagcgcg ccagccattc ctcgtgctgc
     9601 gcgggataga tcttgaagta gttcggcccg tacatcccga acggggcgga gagcgcaaag
     9661 aggccgttgt agtgcagcgc gggcaggtag cgggtcgaga ccagccagac cgcgccgcgc
     9721 tcgaaccggt ccgcaggaag gggcagcgcc aggtccggct gccaaccggg cccgtacagg
     9781 gcatcgaatc gacggacctc cgcgcgcagg gcgggcagcg gtccggccac gagcagcgcg
     9841 gccacgagcg ccaggatcgg cgttctccac gccggccgcg gaagcaggat gcgcaggtgc
     9901 agcgggagca tcagcaggga gatcatttgc ggcaggaaaa aaagccggtg caggtcgttg
     9961 ggcgagaagc gggcctgcac gaaaagcggc agcagcagga acaggcccgc gcaggccgcg
    10021 agcgctttcc actcgggcgg aagccgtttt ttctgaaagc gggcggccag ccccgccgcc
    10081 gggagcagcc acagcgccac cggaaattcc gcgagccagg ccgcccagaa acggccttgc
    10141 ccccagcccg gctcgcccag cccccacccc ggcatgaagg gcggccggat ccgccagccc
    10201 gccacgcccg cgccaccggg gtgaaacacg agcgccgtca gcagcccccc ctgtatcgcg
    10261 gccacggcca gcgcggcggc cgccgccccc aggaagaagc gcgccgccgc gcgccaggcc
    10321 ggcgagagca gcagcccgag cgccacgagc gcggaggcca cggccaaatc ctccgacgtc
    10381 agccacagcg ccgcgttcga caccgccaac gacgcccagg cggccgggcg ccgccgcgcc
    10441 gggtcgcgca atcccggcag ggccgccatc tgaaagagaa agaacgcctg gcccagcggg
    10501 gtcggacggc ggccgatgaa gaggggcagc ggatgacagg cggcgtatac attgtttctc
    10561 cacgtttcca ccgacggatc gaggaacttg ccgagaaaga ccagcccgcc gccgcataac
    10621 atcaggatca tcgccaggat catctcgccg cggccgacgc gccactcctc cggcaggccc
    10681 gcgttgaaac cgcgccacca ggcccatgcc gcggccagga ggaggacgag cgccgcccac
    10741 gacgtcgcgt ccagcgccga ttccacgtgc cttccaggcg ccgcgagcct cgcgaggcga
    10801 aggaaggccc cggccagcac gtcgaacccg tagtgatagg gcagcaggcg acccgggctg
    10861 gccagatcgc gaacgggcag gtttccgcgc cctatgcttc cggtcagcgg gatatgacac
    10921 agccagtcgt gtccgacgtg cgtcctgatc cagagataga gcggcagcga aagcaggaga
    10981 aaagcagcca gccggacggc gcggccgctc gccttgcctt ccgctacctg cttccggcgc
    11041 cagccgacga cgatcaaggc gctcgccatc cccagaaccg ccgccgcatc gaacggcagc
    11101 gggcagccgg cccagaacgc ggccgcggcc cacaggttga tgaaaaaaag gagcgccgcc
    11161 gcaccggcca ccattgtcat ggccgcctcc tcatggggcc ggcctcgtat tgtccacccg
    11221 ctgcatgacc gtgaacggcg tgcccggaat ttcggccatg ccgtactccg cgccgtgggc
    11281 gtcgaaaaaa gcctcgagcg cccgggtctg cgcctcggag tacggcgacc aggccgggcc
    11341 gccctccgac aggatcccaa ggaacacgac cgccggcggc cgctcttcga gttccgagcg
    11401 gtacccctcc ggggtcagcc ggtccgcgcg cgggaatccg gggaggttcc gggcggccca
    11461 ttcgagcccg tcgtgcccgc gtccgtcgaa atcgctgatg attttataaa aggtattgga
    11521 tcggcacagg ggatgccagg gaaacgccgc gaccacccgg tcgtgggggg cggcgcgatc
    11581 gaggagatag cgaagcgcac gggcctggac cgtcctcgtc ggcgagaagc ggctcccgtc
    11641 ggcgaaccgg atcaccggcc cctgttccgc ggccggggcc gtcagggcgc gcagcgccag
    11701 cagaaccagg accagcgccg ccgggccgag ccaccagcgg ggccattcct ccgcggcgcg
    11761 cgcggccagg gcggccggca ggacggcggc gagcaggaac cacgaagcgg tgtactggag
    11821 ccagggcttg gtcgcaagca cggcctggag caggagaaag aggacgacgc ccgtttcgaa
    11881 ggcgcccgcc cgctcgcggc ggcgagccac gcgccagccc catcccgccg ccccggctgc
    11941 ggcgcaggcc agcgggagcg gttgatcccg gagcgcgcgc cagagtccgg ccccgaacgt
    12001 atgcatgcgt tccagggccg cgttgtaggc aacggccgat tgccacgccg cgcgcgggtc
    12061 gatgccgtat cctttcagca ctccggccgc cagcagcagc gcaaggcctg cgccgcctgc
    12121 ggcccagccg gcccgggccc acgccgcccg ccggtcatcg gcgcgccaca gggcggccgc
    12181 cgcgagcacg accggcagca ggaggagttt cgtgttgatg agcatggccg cgacgaccag
    12241 aaaaccgccc gccgccgccc gccgccgcga cggacgcccc cgcagcagcc agagcccgcc
    12301 cgccagcagc ggattcgaca ggctgtcggg cctgaactcg acgaggaacg ggagcaccgg
    12361 cggcagcagc gccatgaacg cgagcgcgcc cagggcgcag gcgcgggcgc cggagtcgcg
    12421 cccccgcgcc gcgtgcgccg ccagcaggag caggaaggcc aggtgcccgg cgagcgcgag
    12481 cccgcgggcc agattcatcg tgcgcagggg atccgccagc cgggacgcca gcggccggac
    12541 gagcatccag aagaagggaa gatggttggc cgcgaagtcg cggtacggga cctggccgcg
    12601 gtccagcagc caggccatgt gcgcgtggct ggcctcgtcg taggtcacga cccgggtccg
    12661 gctggcgggc caggccgccg cggccagcaa agccgcgaca acgaggaacc cgcccctcga
    12721 tatcatgccc cgcctgttca cggcccgtca ttctaaccca ttgcccggcg cggcgacagt
    12781 cccggattgc gttgaccgcg gcccggcggt ccgtatgatg aaggatcgaa acctcaacca
    12841 agggagagca catgagcacg tggacacgca gcggcctgtg gttcctggcg gcggcggtgg
    12901 cgttgagttt ggcgaccccg tccctcgcgg gctggccggc gccggaccag atgaaggaca
    12961 tccgctcgat cgccgagcag ctggtcaaat ggtcgaacaa gctgaaagag gagtaccagg
    13021 gcaagtggcc gggcgaccag gacttcctga accgcgcggg ccgcttctgc aaccggtgcc
    13081 agcacctggt gaacatcgtg aacgtcgcta tcgacaaggg cgagggcgga cggcatacgc
    13141 aatcggccta cggcgaggtg cgcgaagcct ggcgggcggt ctacacggcc tccggcgccg
    13201 gccataccga cgactggaaa gagcggaagg aaatcaacaa gctgatggat cggctcgcgc
    13261 cctactacca gtaagccgct ccggccacgc ggaaaaatac aaaacggccg ccctccgggg
    13321 cggccgtttt tttcatgcgt tgcgcccgct tactcgtcct gcagcaccac gacgcggaag
    13381 tggccgcggc cggcggccag gccggacgac acgggcagtt cggtcagcgc ccctgtggcc
    13441 gtgatgggat cgggcccgct gaccttgctc caggtcggcg gcggatcctc ggcgtccagc
    13501 ggatcggtgg tgtaatagac ttcgtaccgc ttgcccgcca cggaatccca cgtgacgacg
    13561 gcctcgtcgc cggcgccctg ctggatgcgg gccaggatga acacggagtc cggatcgcgg
    13621 ccgtcggtgc cggcaaccca ctcctcccag tcgttcacgc cgtccgtgtc gctgtgcgga
    13681 ttgtccggat ccgtgccacc gctctcctcc caggaattgt tcaggccgtc gccgtcgttg
    13741 tcccgggtgt cgagcgcgta gcgggtcttg tcgatgttga aatagaggtg gccgtcctcc
    13801 gggaccggct gcggcgccgc ttcgccgtag aacgacgcgc cggtcgccgt cgaggcattg
    13861 aagacgcgca caaagaccgg cgtgcccagg taggcgtccg cgtcgcggac ggtgttggag
    13921 accaggacga cgatgctgcc cggctcgtag cccggggcgc ccggcaccag gccgtctccg
    13981 atccggacac aggcgttggt gatgccggaa ggctcggcga tgtcggccat cggtgccgtg
    14041 cgggggtccg ggttgccgtc gtagtcgggc ggatacggaa tattgctggt ggcccacagt
    14101 atctgcacca ggtcgccgtc ttccaggacc acgttgttct cgttcagcgt ggtcgtcgta
    14161 tcgacgccca gcgtgcccgg caggatctgg ccgaaggcgg gcccggcggc gcacagcagg
    14221 gccaggacaa gaactccggg cagatccttc aaggcccgcc gcgttatgag ggaatcgttg
    14281 tctttattca tgacgcgctc gcctccttcg acgccttctc gtccgcccgt tgagatcggg
    14341 attgtttcct gacgctggcg cccataccgg ccagcaacgc cagggaaagc agcatcaggt
    14401 gggccgtgct cggctcgggg atgacgacaa agcagttgtt ggaatcggcc tcccacccgc
    14461 cctcgaagtc ctgctcgacg acgaggaagt tctcctccgc ctcgtagagc ggcgactgac
    14521 cccatgccac ttccccgccc gtgaaatcca gggtgttgaa gaagcggatg tacagattct
    14581 ctatgttcac ttccgtgttg tcccaggtga tcaccatgtc ggagaggaac gtgccgtcga
    14641 tatcgccgcc gccggcgttg tagtacggct gcccgatatg cacgaccccc aacaggatat
    14701 cgtcgtcggt ggcggtgccc ggcaggtagc agtcgttgaa catggccatg gggtcgatgt
    14761 cgccgttggc atccccgtag atgtagacga tcgagccgta cggcagagcc gtgatcccat
    14821 cgtccagcag cagcgccgaa ctcacgtagg cgatcaggct gatgatgtcg ccgcgcgcgg
    14881 gcacactggt caggcagacg atgccgatcg ccaggcccag caaccatctt ttcgctttac
    14941 gtccggtcct catatcacag ggccagttgc tcaaatcaac cgacaccccg cgctcccggc
    15001 cgcgtgaaac agccgatgcg cgaagtatcc aaattacacc tctggattca attactagag
    15061 taccgaggat gtccccggct gtcaacagag cccgcgcggc ccgaatgggc acacggcttc
    15121 gcttcacctg ccgggaccag gagatattca atctattaat ccatataggg ttatgactgg
    15181 cgcgggcgcg cggtttacag ggcggtagcg gagtggtact tatagtacat gtcgcagttg
    15241 ccaccatcag gcccgcgccg ggcaagccga ccgggcgggc gcgccgccgg cacgatccgg
    15301 gcgctcatcc tggccgggca ggccgcgtgg gcgctgggtg cggccgcggc catccacgag
    15361 tcggatcaca cgttctggct gaccatcgag acggcgccgc cgggggccac ggtgctcgcc
    15421 gtgcccgggg acggacggga aaaggagcag gcgctcggcg tgacgccgtg cgcgctggcg
    15481 gtggatttga actgggaagt ccggtggttc aagaagcggt gggagtcgct gaccatcctg
    15541 tcgcccggag ggttctgccg tccgacgttc aacacgaacg agggctaccg cctcaccgcg
    15601 cgcctcgtcg ttcgcaagga aggctggaag gatatcccga tcgatacgca cctcgccacg
    15661 ctggcggacc cggggaagga ttggagcggc aaggagcagt ggcctgcgaa gcagacgctg
    15721 cggtttgtgc tggaagcgct ccccccggca tcgcccccct cctccgcggc ggcccgcgcc
    15781 gcgccgcgcc gcgtggtcct ggccggcgcc ggggaaggat ccggcaaggt ggagaccggc
    15841 cgggtcacga tcttctgcga gacggcctcg gcggaggtct gggtggacgg ccagtccgcg
    15901 ggaagcgctc ccgtcgagct gctcctgcgc gcgggcaagc gcctcgttga ggtgcgcgcc
    15961 ggggaccggt tggtctaccg gcgcgaaatc gaactggagc ccgacgcctc gctctccttc
    16021 atagtcccgc cgggggagtg aacgcgccgc ccgctcaaga cgtcatggtg tcgccgaact
    16081 cgtcctccag ctcgaccggc ccctcccgcc ccttgtcgcc gcgggtcagc agccagtgga
    16141 ccaggccgaa gaggccgatg ggcaggaccg cgccgaaaaa gatgagctgc aggaagttga
    16201 aatggtacag caggtcgggc gtcatccggt acaggttcca gaagatcagc aggcagctca
    16261 accacacggc gctgaaattc ggaaccgcat cgccggagcg catgacgtgc aggcctatga
    16321 accgcctctt cgtgatcggg aagaacaggt tggatcccat gaagccgagc tggtcctcca
    16381 ggaggtgggc cgcgtagccc gccatgatca ccaccgccgc ctgccagctc cagagcagcc
    16441 agcccagcag cgcccaggcg gcgccgacga agaagctgtg gctccagttg cggtgccacg
    16501 gcaggaagtg caggaccacg cgcccctgcc cgtccgcctc cagcgcaaag gtcgggccgt
    16561 cgaaaatatc cacccgcgtc gtggcgtcgt aggtctggac gatcggggcg gccagccggg
    16621 cgcggccgat cttccgctcc gccggctcgc tgttcggcac ggggacctgc ccggtgttga
    16681 cgacgggccc gatgcggacc tgcacctcct ggttctccgc gtcgaaccgc acgatgtact
    16741 gccgccagta atccgcgccc atccgcacgg tgttgagctt gatccggacc gcccgccccg
    16801 ccgtccgcgc cttgtccacc gccctggcca gctcgtcggc gatggcctgg ggatcggggt
    16861 tctgcgggtc cgggtcgacg tagaagtcgt gccggtagaa gaaccggtag aacttgaagt
    16921 cgatcgtgtc cggcaggatg ccgaatgcgc cgccgagaat gaagtagagg ggattgcctt
    16981 ccgcggcggc ccggaccgcc cacgggcaaa acgacgccgc catcaccccg ctgacgaaat
    17041 gggatatgcc tttcatccgg gtcctccgtt caaatcagcc gcagccacat ggcgatctgc
    17101 tgcccgaagc cggacatgtt gcaggcgatc ggcagcagca ggatgccgag gcagttcagc
    17161 cggcgcgacg cgaacagcac cgggatcagc ccgatgccgg tcgccacgat catcaccagg
    17221 atcccgcccc atccggtcat caggtacacg atggccagga tgatcaccag cgcgacgagc
    17281 gacgcgttgc ggtagtgcac tttctcgatg aaccggatgg tcgcccgcgt caggggcccc
    17341 atcatgagga aggcgaccgc gccggagatg gcgatcgcgc ccagcaccat gaagtagtcg
    17401 tagtagctgc gcgggacata gaggcccttc atcatccagg ccgcgccccc gcgggtcagc
    17461 cccagccccg gcacgaagag cagcatgaac gagccgacgt agtacacgag cttcgacacg
    17521 ccctgcgaca tgatgaagac cttctcgtcc cgctgcgcgg tcgcgtggcc ggccagcagg
    17581 cccccgatgc cgccggtgat gaccgggaag aacgccgcga agccgccgcc cagcccgccc
    17641 gccgccgcgc tgcgcaggat caggtcgccg ttcagctcca gcgagtgcgc gatgcgctgc
    17701 ggcggtacgt tggccccgct gatcatgttg agcaggcacc acggcacggc gaagaggccg
    17761 acgaaggcgg gcatgatgtt ctggaaggcc atgtccaccg acacgggcga acggtagagc
    17821 aggatgaacc cgagcaggcc ggagaggaag aacgtcgcga gccccgcccc cagcgagcgc
    17881 caggcgtccg cgaacttggc caggcccgcc ggcccccggt tgccgccctt cggccactcg
    17941 gacatgagga tgtacgtgat gatcacccag aggatccagt gcatgtgcgg ccgcaggacg
    18001 ccttgcacca cgggcagcac gcgcggcgcg aacgggccga ccacgcagac caggaagaag
    18061 acgcccgcca ggccgccgac gccggtcatc atcgtgccct cgtacccgcg gccgctcatc
    18121 aggtacttct ggccgggcag caccgtgaac atggcgctct cgtccggcgc gcccagcagg
    18181 atggagggga tcgtgttgag catggaccat ccgacgacga tgcccgccat gaacggaagg
    18241 aagacctcgg aggggatcga catccccttc tcgatcagcc agtagaacag cagcaccagc
    18301 gcgcccagca cgttgtaggc gtgcagggcg ggcagcaccg agatcgtgca cgacagcacg
    18361 gtgcccagga tcgtgacgcc cagaaccgtc aggatgttca gcatcatcgg gtcgcgctcc
    18421 ggttattcca gctccacggc ctgcgcgctg gcggggacga tctgcaactg gccctggtaa
    18481 tcgccgatcg cgcccgtggc cttcacggtc cggcccggct ccagcttgtc ccgctcgggg
    18541 cccgggacca tccggtccca caggagcagg tcgatctggc cggtgccgtc gctcaaggga
    18601 tagacgatgc ccttcatcag cgaccggggc tcgcccagcg cgccgcgcac ggtgaacacc
    18661 cggcccttca tgtccgccgt cagcttgccg atctccacga ggccttcgcc cgcgggggcc
    18721 ggcgcggccg ggggctccac gtccaccagt ttcaccttcg cggaatgggt gaccttgatc
    18781 tgcacggtct tctcgtagac atccaccagg ccgcggacct tcatcagcac gccggtggac
    18841 ggcgcgttct ccccgaggcg ggtcgccacg tcatcccagt agaccacggg gatccgccgg
    18901 ccctggtcct ccaggaccat cgtgtacgga gcccgggagc ccggcgccgg cgcgcgcagt
    18961 tcggccacgc ggccgagaac cgtgatttcc ttccccttca tgtcgacggt gacatcggca
    19021 atctccgtcg cggcttcgac ccgcgtgggg acggaacgcg gcgcggcggc ggccggcgcc
    19081 ggcttttctt ccgcgggagc gcccggcccg gcgacctgcc gctcggcgga atcggcgcgg
    19141 acctcgagat cctccccgcg gttcagcatc agctggagtt tgtccttgta ggtgcggacg
    19201 cccacgcgcg cccgcacggc ggccccgggg atcagcagcg acttgtcccc gatatccggg
    19261 tagaggtccg gccagaaggt gatggctcct tcgccggaat cgtcccggag ttcgatcacc
    19321 cacggctgcc ggctctccgg tttcggcggg ttgacggccg tgatggtccc ctcgaccagg
    19381 atgctgttgc ccacgagttc gccgttgacc tcgcccagga ccgtcaccgg cacctccgcc
    19441 cgctccaact ggaggtgctc ggtcacctgc agccgcatca ccacgtcgtc gtccgccgtc
    19501 atgctcaagg cgccggccac cgtgacgcgg tcgcccggcg acggcacgag gtcctgctcc
    19561 accagggctt cggcctggga gcggaaggcg gtcaccgaaa tctcgcccgt gccgtcgtcc
    19621 acggaaaagc gcagcgaccg gaccttgccc gcctccttga agacgcgcgg ctcgccggac
    19681 acggcgccga cgacgcggac gtacgcgaaa ttcatcgtcg gcttgatctc gccgatgcgc
    19741 accaccggca gttcgcggtg cgtggccatc aggtacagca ggcccaggcc gatcgtgccc
    19801 agcgccagcg ccgcgtaccg gaagacccgg atgctcatgc gcttctccac gcgcgcgccg
    19861 cagcgcgggc agcgggtcag cgccccgaca aactggccgc actgggcgca ggtgatctcg
    19921 gccggctggt ccgcgtgccc cggcagggga ttcgtcgtca tggctttcct ttccgagccc
    19981 ggccgccgga agcggacccg tggcccattt gggaaggttg actatatcgt ggctgttccg
    20041 gcctgcctag cggattcttc tggacgcccg agggatcgct tgccaccatg accgcgtgat
    20101 ccgagcatcc cgccagcgcg cggacaacga gcgcgcgcgc cgccgggccg agcaggccct
    20161 ggctgaaagc gcgcgggaat atcctctgct ggccgaatcc ctgcccgacg cgctcttcat
    20221 caccgaccgc agcggactgg tctgcttcgt caattcggcg gcctgccggt ttttccgccg
    20281 ccccgtttcc gcgctggtgg ggaagaggca gaccgacctc tttcctcaac ccgtggcccg
    20341 ccagcatacg cgggccatcc agaaggtgtg ccggaccggc cagccccata tcaagatgga
    20401 gccggagcgc ctgccgggca ccgacctgtg gattgagacc cgcctccagc ccatccgcaa
    20461 ccggcggggc cggatcaccc atgttctcgg cctcgcgcgc gacatctcgg cccgcgtgaa
    20521 agcggagagc gccctccgcg aaagcgagga gaatttccgg gcgatggcgg agaatggaat
    20581 tgacgccatc ctcatcgccg tgccgcccgg ccaccaggtg tttgtcaacc ggcgcctggc
    20641 ggaactcacg ggctattcca tccgggagct tcaccggatg catatctcca ggctggcgcg
    20701 ccccccggaa gcccgccgag tgctggacat gttccggcgg cgcttgcggg gcgaggaggc
    20761 gccccagcgt tatgaagcca tgatactgcg caaggacggg agggagacgc ccgtggaggt
    20821 gtcggccacc cgtaccctgt ggcaggggcg cgcggcggtc atggccatcg tccgggatgt
    20881 cgcggtccag cggcgcctgg ccgaggagcg ccggcgcctg gccagccggc tgatcgagat
    20941 ccaggagcgg gaacgacgcc tcatcagcag cgcgctgcac gatcacctgg gacagatcct
    21001 gacgctggcc cgcctggaga tgggggccgt gcagccggcg cgccgcgatt ccatcctgca
    21061 tcgccagaag gcgctcctgc gcctggacga ggcgctggcc acggtccgca acctcgccac
    21121 gtcgctgcgt cccccgatcc tggatgacct cgacctcgaa accgccctgg aggacctggc
    21181 cggccagttc ggcgactccg gcatcgacat ccgcttcagc catcgcgggc ggccctcccc
    21241 gcagagccgt cagatcaaga cctgtctgta ccgcgtcacc caggaggccc tgaccaacgt
    21301 ggtgcgccat gcgcaggcca cgcgggtccg gatacacctg gacgtccgcg cgggcacgac
    21361 ccgcctgacg attcgggacg acggcaaggg ttttgatcaa gccgcgattc cggagtcgca
    21421 gggcatcggg ctggccggca tgcgcgagcg gctgcggttg tgccgcgggc gccttgaaat
    21481 ccggagcgtc aagggccggg ggaccggcgt cgtggccgtg atcccgccgc ccggttcacg
    21541 cgtcatcctg gaggacatcc gatgattcgt ctcatgctgg ccgacgacca tgtcatgctc
    21601 cgggagggtc tgcgctcgca gctcaagcag agccccgatc tgcaagtcgt gggcgaagcc
    21661 ggcacgcccg ccgaagtcat ggagcaactg ccctcgctga agccggacgt gctgatcctg
    21721 gacctgaact ttcccgatgg aaacggcatc acgctcctcg ggcagatcgg ccaggcctgg
    21781 ccctcctgcc gcgtcgtggt gttgaccatg tacaaccacg tgcgttacgc gcagcacgcg
    21841 atggaaagcg gggcgcgggg ctttgtggtc aagggcgcgc ccttcgagga actggtcgag
    21901 gccatccggg ccgtgcaccg gggagagacc tacatgtgtc gcacgatgcg ggggcggctt
    21961 gccgcgcaga aacgccgggg gcgccgtccg gacgccttgg gatccctgtc tcaacgcgag
    22021 ttcgaggtgc tggtcctggt ggggaacggc ctggccatga aggaaattgc cgcccagctc
    22081 ggtgtcagcg agaagagcgt gaccacctac cgcgcgcgca tccagcagaa gctgcagatg
    22141 agaaacaagg ccgaactcat tcgttttgcc ctggaaaacg gcctcgtgga gtagcccccg
    22201 gcgtaggcat aatcctacgg cccgccaccg gtaatgcagt ggacaaaggc gccgccgatt
    22261 tgtgaatcta tcgtttcggg tggacgccat ctgttttgag cttcactcat gcccgcaacg
    22321 caggggccgc cggtgggaag tgaggacggc ggaagggtgg gctgaacgtg aaggccgtaa
    22381 gtgaagcttg agtgcccgcg cggtttccgc gcgggctttt tttcagggaa aaggcgcatg
    22441 gaactgcatc ccaccatgtg gcgcacgtgc cgggtcctgg ccggaccgac gcgcctgcgc
    22501 ctgcttcgcc agatcatcgc cgagccgggc ttacccgtct ccggcctggc cgatcgggcc
    22561 gcgttgaaac tttcgcgggc cagccaggag cttcgccggt tgagttcgcg ggaactcgtg
    22621 caggcggtcc ggcagggccg ggaggtgcga taccgtcctt atcccgaccc gcaggttccg
    22681 tcggcttcgc ccctgctggc ggccttgacg gcagccctgc gccgggggga cgcgatggcg
    22741 gcggattgcc ggcgtgttgc cgcggccttc tcgcatccgc gccgcatcgc catcgttcga
    22801 gagttgctgc ggggcccccg tccggctccc gaactcgcgc aggctctccc catgcactcc
    22861 cggacattgt accgccacct gcgctggctg aagcataccg gcacggtcaa ggaagagggc
    22921 gctcgttttc tctttgtccc ggatgcgaat cccctgttgc gctgcatagt ccgccttctc
    22981 catcgccaac gggagtgagg cctgccgctt ttcattcccg gttcgcaggg cggtgacatg
    23041 ttttataaaa catgtcactc gggccgcgac gccttcccgg cttttttctt ggcgggcccc
    23101 accccggcac ctatgctcgc gccttgttca ccatggcgga taccgtttcc atacgaagtc
    23161 tccaggagcg cctcgagcag gtcgtctcga ttcgcggctg gctgtacggc aagcgttcgg
    23221 gcggacgaat cgccttcctc ctcgttcgcg acggcacggg cctgtgccag tgcgtagtcg
    23281 aagcggcgca ggcggagctt ttttccgccg ccgaatccct gggccaggaa tcgtccctca
    23341 tggtcacggg acgcgtccgg gccgacagcc ggtcgcccgg cggcgcggaa ctcgccgtga
    23401 ccggcctgca ggtgcttcaa tccgcctcgg actacccgat cagccgcaag gcccacggca
    23461 cggatttcct gatggagcac cggcaccttt ggctccgctc cccgcgccag gccgcgatcc
    23521 tccgcgtccg ccacacgctc atccgcgcgg cgcgccggta cttcgacgac cacggcttca
    23581 ccctcgtgga tacgcccatc ctctccccgg gcgccgccga aggcgcgggc acgctcttcc
    23641 ccgtggatta cttcggcgac accgtctacc tcgcgcagac gggccagctc tacctcgaag
    23701 ccgccgccat ggccctcggc cgcgtctatt gcttcggccc gacgttccgc gccgagaaat
    23761 ccaagacccg gcgccacctt gccgagttct ggatgatcga accggaaatc gccttcgccg
    23821 agttgccgga actgatgacc tgggccgagg gcctcgtctg cgccatggtc ggcgccgcgc
    23881 tctccgagcg ccgcgaggaa ctggccctgc tcggacgcaa ccccgccgcg ctggagcgga
    23941 tcgccccgcc cttcccccgc ctgacctact cccaggccgt ggacctcctc cgcgaccccg
    24001 cgctgcaccg caaactggag caggaactcg aggccgaccg tgcgcggctg aaaaccttga
    24061 tccctgaact cgccgccctg gacgaacagc tttcccgcgc gcgccagggc tggcagcagg
    24121 acaagctggc ccaacaggcg gccgacctgc gcgaacggat ccgcgacctc gaggcccagg
    24181 tcgcccagcg gcccatccat atcgagcacg ccaggaattt cgcctggggc gaggacttcg
    24241 gcggcgacga ggaaaccatc atctcccgcc agttcgaccg ccccgtgctg atcaccgagt
    24301 atccgcggcg catcaaggcc ttctacatga agccttcccc ggccaacccg gacgtcgtcc
    24361 tgaacctcga catgctcgcg cccgagggct acggcgagat catcggcggc agccagcgcg
    24421 aggacgacct ggccgcgctc cggcagcgca tggccgagga gggcatgaat cccgccccgt
    24481 acgagtggta ccttgacctc cggcgctacg gcagcgtacc gcacggcggc ttcggcctcg
    24541 gcttggaacg caccgtcgca tggatctgcg ggctcaagca catccgggaa accatcccgt
    24601 tcccgcggct gatggggaga atctatccgt gagcaccagg ccgtcccgcc ccctgaaaat
    24661 cggcgtcctc ggcccgcaca aggcgacgga cgatgaactc cagttgggcc gcgaggtcgg
    24721 cgcggaaatc gcgcggcgcg gggccaccct gctctgcggc gggctggacg gcgtaatggc
    24781 cgcggcctcc gagggcgcga aaacggccgg cggcctgacc atcggtatcc tgcccggcga
    24841 cacggccgac ggcgccaacc ccttcatcga catcgcgatc ccgacgggcc tgggcgccta
    24901 ccgcaacatg ctgctcgtcc gcgcctgtga cgcggtgatc gccgtgcgcg gcgagtacgg
    24961 cacgctctcg gaaatcgccg ccgcgctgcg gctcaaggtg cccgtggtcg gcttgcaaac
    25021 ctggaaactg gtccgcaacg ggatcgagga tcccgggctc cgcgtggccg ccactccctc
    25081 ggaggccgtg gacctcgccc tccgcctggc cgcctcgccg cctaccgcgc gaacttgaga
    25141 aactccgcct cgatcgccgc gacggaattc agcccgtagc gcgcggcgaa gacggcctgg
    25201 tacccctgcc cggccgcgag atccgcgaca aacggcacga accggtccgc gggccggtcg
    25261 agccgcagcc agccgaccag gtacttcgag gtttcgtaaa aggcgtggac ggacgcggtg
    25321 tcgttcggat aggccgacgc ctccagcagc gaggcgaccg ggaacacgcg attcagccgc
    25381 cggaactgcg accggcggct cttcttgatg cccttgaagg cggagtacgc gaactcctcg
    25441 tagaactccg cgaggccctc gttcagccac agcggcggcg gccgctcgag gaaccggttg
    25501 accaccaggt gcgccatctc gtgcgccaac acgtcgccgc tggacgaggt gtccgccgcc
    25561 tgctggagga acatcaccgg ccccgcgacc tgggaaaagg accattcggg cacgtcgccg
    25621 gccgacgcgc cgaatgcctt ccagtccttt tgcgtgcgga agatgaagat gtgcgagcgc
    25681 ccctccagcc ggtccgtcgc gccttcgaga tcggccccga tgtagccgta aaagaactcg
    25741 gccatccggc cgactttccg ggcgaagatt tcctgctcga aatggacgac gaaatggggg
    25801 gtgtgcgcgt gccgccactt caggccgggc atgtcgaggg ccgtctgccc ccgcggcgtc
    25861 agggccttcg tgtcgacctg cccgcgggcg acctcctgcc agttcggatc cgcgggcggc
    25921 gcggccgggg cccgggatgc cgcgaccagc cacgcggccg cgaggactgc ccgcccgagg
    25981 cgcatgacga tcaggacgcc ttggcctgcg ccgagagcca gacgatgtgc cgcatctcct
    26041 cgtggatgat gtcgtcgagg cgcttgcggc ccagggccgg cgcggcggcg tcgcgaagcc
    26101 ccgagtaaaa gagcacggcg tccttctcca tctggatggc cgcgcgcagg atgtccgcga
    26161 gcggttcgcg gccggtcaac gcgtcctcgg gcgactggtt gatctggatc ttgaggtggt
    26221 ccaccatcgc gtccagatac ggggccaggt ctccgtacgg gtcctcgccc tccggtttct
    26281 cggcaggcgt cagggagcgg cgcatgtcct cgaagaactg gtaatgctgg tcctcctggc
    26341 cggcgatacg gttgagcagc tcgcggccgg ccacgtgcag tttcgcggcc ttgcggtagt
    26401 agcgcgcgcc cttctcctcc aggcgctggg ccatccgcag gatttcgtcg gcggtcagtt
    26461 tagtggacat gcgggggtct ccgatgtgtg tgaaatcagg ccttgccgcg ggccgcgtga
    26521 agctcccggg agagctgaac gacatggctc ttctcttcgg caatgatctt gtccagcttg
    26581 tcccggccca ggctggccgg cacccggtcg cgcagcccgg tatagaactg caccgtcttg
    26641 tgttcgagat cgatcgccac gcgcaggacc tgctccatgc tctccttgcc cgtcagcgcg
    26701 tccgcggcgg tcggcgagcc ctcgcccccg tgctggtcgg ccagcgcctt gagataaagc
    26761 agctcctcgt ccatcgggtc gtaggtcgtc ggctccttct cggccgcggc cagctgcgtg
    26821 cgcatcagcg aaaacgtttc ctggtgctgg tcctccatcg ccgcgagctt gaacagcaga
    26881 tccttgctcc gctcgccgga gtgcagcttc gcggccttgc ggtagaacgc gcccgcgttc
    26941 ccctcgatgc gctccgcgac cttcaaggct tcgttggcgt tgaacgtaat cggcatgtcc
    27001 tgctccttcc ctgtttcgat ggcttccgca acctagcaca ggacccggcg aaaggcaaag
    27061 cctgccggcg gttcagcaaa tccgcggcag gtcctccccg ctcggcgcgt ccaccatccg
    27121 ctcgccgccc agccgcgtcg ccagccgcac ccggcccgcc gggcccttca ccacctcgcc
    27181 gatcgccgcc gccgcccggc cgcgcggatg cgcccgcagc gcggccagcg cgcgctccgc
    27241 gtccgccgcc gcgcagacga ctaccgtctt gccctcgttc gccacgttca gcgggtccag
    27301 ccccagcagg tcgcatacgc cgcgcacctc gtcgcgcacc ggcagcgcgt cctcccgtat
    27361 ccgcaccccc gcgccgctgg cctccgcgat atcgttcagc gccgccgcca gcccgccgcg
    27421 cgtcgggtcg cgcagacacc gtacgcccgg cacggcgtcg agcaggccgc ggaccatgtc
    27481 ccacaacggc gccacgtcgc tctccagccg cgacgaaagc ccccacgcct cgcgcgcgct
    27541 catcaccgcc accccgtggt cgccgagcgt gccgctgacg agcaccgcgt cgcccggccg
    27601 cgcgttgccc acgcccgtgt ccgcgccgcg gtaccgcgag ccgagccccg ccgtattgat
    27661 gaacagccca tcccccgtcc gccgctcgat caccttcgtg tcgcccgtga cgatccgcac
    27721 ccccgcctcg cgcgccgtcc ggccgaccga ctccatcgcc cgccgcagca cgtcgatttc
    27781 cagcccctcc tcgatcacca gcccgagtgt cagggccagc ggcgccgcgc cctgcatcgc
    27841 caggtcgttc accgtgcccg acaccgccag ccgcccgatg tccccgccgg ggaagaacag
    27901 cgggcgcacc acgtacgaat ccgtcgtcac cgccacctcg cggtccggcg tctcgacgta
    27961 cgcggcgtcg tccagtttct cgctcccgtc gtagcccagg gccggcagga tcacgtcccg
    28021 cagcagctgc ttcgtgcgcc cgcccccgcc gccgtgggag agcaggattc gttcggaggc
    28081 ggattttcca atggttggaa aagtcatggg tctatttttc caatgtttgg aaaatttgcg
    28141 ctattttttt ccaatggttg gaaaaatccc ggcgtttttt tccaatcatt ggaaacccgg
    28201 cgctacgtct ttccgaaccg gtaatacgcc gcgcaggtgc cctcgctgga taccatgcag
    28261 gcgccgacgg gctggtccgc cgtgcagacc ttcctgaaca gcgggcattc cggcggggcg
    28321 cacaggccgc gcaggacgtc gccgcaccgg cagcccttcg gctcgcgggc gggcggccgg
    28381 tccagcccgg ccagcagccg ctccgcgtcg tgggcggccc actccggccg gatgcgcagc
    28441 ccgctgccgg ggatcacgcc cagcccgcgc cacgcggcgt cgcactcctc gaacgcctcg
    28501 cggatcgccg cctgcgcctt cgcgttaccc tcgcgcgata cgctccgggt atactgcacc
    28561 tcgacctccg cgcggccgtc cgcgagctgc cgcagggcca tccggatcgc cgcggcgagg
    28621 tcttcgccct cgaagcccgc gaccacgcag ggccggccgt actggcgggg aatgaattcg
    28681 tagggcgctg agccgatgat cacgctcacg tggccgggac acaggaacgc gtccacccgg
    28741 acttcgccgc cggcgagcag cgcggccatc gcgcgcggca tggtcttgtg cccgcacagc
    28801 acgaagaagt tgggcgtgtc gcgggcggcc tcgcgcaggg cccacgcggt ggtcggcgcg
    28861 gtggtctcga acccgacgcc gaggaagacg acggcggtcc cgggattcgc ccgcgcctcg
    28921 gcgagggcgt cgagcgggga gtacaccacg cgcacccgcg cgccgcgggc gcgggcctgc
    28981 tcgagcgact cccggctgcc gggcacgcgg atcatgtcgc cgaacgtcgc gacgacggtg
    29041 tccggccggc cggcgagcgc gacggccttg tccacgtagt cgatgggtgt gacgcacacc
    29101 gggcagcccg ggccggagag cagtttcacg ttgtccggca gcagcgagcg cagcccggta
    29161 cggaacgcgg ccatcgtgtg cgtgccgcag acctccatca actggacggc ccgacccagg
    29221 gaatgggcca gcgcctgaat cgcgcggatg gactcgcccg cgctcatgcc ccgcccatat
    29281 ccgccagcgg cttcagcaga tccctgtact cccgcacatc gtcctcggtc cactttcgga
    29341 tcgcgaagcc ggcgtggagc agcaggtaat cgccgggccg ggcgtcgtca atgaatgaaa
    29401 tattcgcctc gcggcggacg ccgccgatct cgacgacggc
//
LOCUS       DAOOTK010000028        28079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1196_length_28079_cov_4.155224, whole genome shotgun sequence.
ACCESSION   DAOOTK010000028 DAOOTK010000000
VERSION     DAOOTK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28079
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1196_length_28079_cov_4.155224"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PK634_05130"
     CDS             <1..1416
                     /locus_tag="PK634_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97152.1"
                     /translation="DEVLPDNDCWLVASRYGALSTVDGVPMIFYGQEQGIQNYNSSDP
                     GGTWYYDGFREDHEENFGKYIPHFKKWNQLTVWSNPPPNSGGIAEWYGKVNRARRLSP
                     ALRSHNRYFLSKVGGGEEARILAVAKYETANAGPAASDVVLAFSLLLRHGEAHTGSSA
                     TYNLQPVWTLLGLNTGKSYNVRNLAASNPDQLLTSGWPKTGASLYNDGIWVNLPADTG
                     GAITDDGALVQYLKLEEVSTNAAPSISLPGPHWLAVGSATNFSVTASDGNGNAVTLTN
                     TVKPSGATFAAGVFAWTALPAELAGTTQAVVFVADDGQGATNSIVTNATQIVVPFDSD
                     GDGMPDGWEWDSFASLTNPPAGDWDGDELSNLDEYTAGTTPTDGDSIFAVETPTPAGT
                     ISVQVPVRTVPGRKYTIYFADDLGGDWSPFANPAQGIGTWTETGGSPSVFSFTDDGSA
                     DTTAGPPLEGRRFYRVKVEKP"
     gene            1452..2651
                     /locus_tag="PK634_05135"
     CDS             1452..2651
                     /locus_tag="PK634_05135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOW97153.1"
                     /translation="MGEERVMVRLKPGREKPVRRGHPWVFSGAVDSIEGAGAPGSVAD
                     VVAHDGTWLARGLLNPGAALQLRIYTRDTAQALDASFFQARLDAALAMRERLFQFAGG
                     RTNAWRLVFSEADGLSGLIVDRYADVLSVQVGAAALVPHLQAIVDHLAARTGLAMHLR
                     IEPDAATREAVDVSGVATPGPSAPGRVIIRENGLSFAVDVAGGQKTGFFLDQRENRLR
                     VAAYARGRRVLSAYCYTGAFEVYAAAAGAVSIVGLDRSEPALELAREQHRLNATTIPV
                     EYRAADVPEALRRYRDAGERFDLIILDPPRFVASQAQLEKGLRAYKDINLLALKLLTP
                     GGILATFSCSGLVSAEAFRTMLGWAETDAGRRLRIGERLTQPADHPGLTSFVEGDYLK
                     GLIGYVE"
     gene            2804..3022
                     /locus_tag="PK634_05140"
     CDS             2804..3022
                     /locus_tag="PK634_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97154.1"
                     /translation="MSKGSGRDVSDCSADQWVRNTRLFRRWECMKQEIQLHKWYESEK
                     AGHDIGWERACVDWMIRFGHHHPEDSIP"
     gene            3129..4223
                     /gene="pyrB"
                     /locus_tag="PK634_05145"
     CDS             3129..4223
                     /gene="pyrB"
                     /locus_tag="PK634_05145"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOW97155.1"
                     /translation="MAKLYRDNPLRKIAWEDFHTLDAAEKGPFFLREGRPYHALNAQQ
                     FTRPILDGLCNLATQVRRIAKTRGGVLFLQDLLCEKRAMLYFAQPSTRTFLSFYAACQ
                     LLGLNPAEVRDTTTSSEIKGESPEDSIRTFSSFFDIIIMRHPAGGFAERIAWLLSNTD
                     RPVPVVNAGSGKDQHPTQALLDIYTLQRSFEKREGIERKKIAFVGDLARGRTVRSLAW
                     LLTLYPGVKQYFVAPPSLQIGPDILAALDRAGMAYELSSDLQAVIPEADAIYMTRIQD
                     EWDTAHESRDVDTNRFHFRVADLARLRPDAVIMHPLPRRREIEPGVDGDPRAVYWRQV
                     RNGMWIRAALILTLFEREREIERYYQDLTS"
     gene            4240..4647
                     /locus_tag="PK634_05150"
     CDS             4240..4647
                     /locus_tag="PK634_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOW97156.1"
                     /translation="MLSKPKSDQLVLRMAKLGVREHDLDEQFIRGSGPGGQKINKTSS
                     TVWLRHRPSGIEIKCGRERSQVLNRYLARRELCDRLEEKILGAKSARQQAIEKIRRQK
                     RRRSRRQKERMLADKRHHSGIKQSRSRSFLSDS"
     gene            complement(4657..5142)
                     /locus_tag="PK634_05155"
     CDS             complement(4657..5142)
                     /locus_tag="PK634_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOW97157.1"
                     /translation="MKDLARRVCVALATGLGLGYSPFAPGTVGSVWGVLIVLLLFPSL
                     PVARQVLLCLGLALVAIPICDVAERHFKKKDPHCIVADEYLTFPISLIGLPVSWSAWW
                     ILPVAFFSNRFFDIVKLPPANQLQRLRGGLGIVIDDVFAALYSLAANHAAYYLLRRWL
                     G"
     gene            complement(5139..6737)
                     /locus_tag="PK634_05160"
     CDS             complement(5139..6737)
                     /locus_tag="PK634_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97158.1"
                     /translation="MIRRRPLLFWLLLAAALGAAAWWVLYTPYRPEHMYRALPAQTAF
                     VSVHRDLAGRWDTIHRNPLIQTLFLAFGVKPEELNELSADPVARQWLQQLASDEALVA
                     YVPSLGTPGEPAWIIVTWLGAKSQRLRWNLRWAKIRELTAHPPYRGWPSWTLDTGGED
                     EPKLTFALVEGMGVGVFSSDPRLLNEALDALDGVQPSLEDQPDFPGRTGIWCRDPKAP
                     DRGWVNPAAVSGGVTSPVGAVSYEWSELQSGGLRGCVTGRDPFAVEFSRAPRLKAGGL
                     DRLLGDLPQGLALLSPSLVLPYMEQPKRAAWMRLMAEIIRLEKAGVVALALLGDDYSG
                     RLKGIKLPTLVGALPVGKSELVSAWLREALDRLNARYRWGLVPREVAAGRGRMTIIEA
                     TSTNFLAGLPPSECPAYMLRDGWLLLASNSEGLEKLADRFDALPTNRPPPVSWMRDLD
                     NEPAPVYVWLDLARAGKTLRMALSTYSLKLLLEDPGGSQETRQKLNEAKAWVDSLMPL
                     SRARFWMRSDGTRMEVSYKLGGER"
     gene            complement(6734..7648)
                     /locus_tag="PK634_05165"
     CDS             complement(6734..7648)
                     /locus_tag="PK634_05165"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOW97159.1"
                     /translation="MGIDLSVEIGGIRMRNPVMVASGTFGYGPEYADLVDLNRLGAIV
                     VKGISPKPVMGNPPARTVETVGGLINAIGLQNPGLEGFLRDGLPFLRGYNVPVIVNIW
                     GRTVEEYEEVAAGFEGAEGVAGLEVNVSCPNIKEGSALFGGDPALFRRVVSAVRAKTR
                     RPLIVKLAPDLSRVAEYARAAEGCGADAISLINSIPAMAIDIETRRPRLGNVSGGLSG
                     PAIKPIALKMVWEAARAVKIPVIGIGGIACAADAIEFLLAGATAVAVGTATFLNPAAA
                     IEIAEGIGDYLARHGLGHVRDVTGGLRS"
     gene            complement(7661..8440)
                     /locus_tag="PK634_05170"
     CDS             complement(7661..8440)
                     /locus_tag="PK634_05170"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOW97160.1"
                     /translation="MENAQILEHRDIQGGYKRLVLRAPGIAPEVRPGQFVHLRVPHLE
                     AAVLRRPFSVFDARDGALSILYKDAGHGTAVLKGAAPGETVSLIGPLGRGFPAECGGR
                     MPVLVAGGYGMAALHLLARALPRRGIAFFGGKTADDVLCVDEFEALGWPVRIATEDGS
                     LGERGLVTAPLDEWMKKPPREGVELFACGPMGMLRAIGERAIAGGWKAWLSLDRHMGC
                     GVGACLTCVQKIHAPDGGWTWQRVCREGPVFESREIVWEDE"
     gene            8556..9359
                     /gene="hemW"
                     /locus_tag="PK634_05175"
     CDS             8556..9359
                     /gene="hemW"
                     /locus_tag="PK634_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOW97161.1"
                     /translation="MPGLYIHVPFCARKCAYCDFYSVPLNAAAAEEYLRALDRECGGL
                     PDGFAPETVFIGGGTPTVLPPAELARLLDLLARRVNLGRVTEWTCEANPVTLDAEKIR
                     ILRGAGVNRVSLGVQSFDDGVLKFLGRLHTAREAVEAFRLLRAEGFDNIGLDLMCAVP
                     GGAIERDLERALALGPEHLSVYGLTFEDGTPLARRRDAGEVAELDEDEQLRQFRVVRE
                     TLKGAGFHHYEISNYARPGRECRHNLLYWGPGEYLGLGPAAHSHWGGQR"
     gene            9375..9650
                     /locus_tag="PK634_05180"
     CDS             9375..9650
                     /locus_tag="PK634_05180"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97162.1"
                     /translation="MEDWAAGAPREFDETLDPEARAREALVFGLRRLDGVGRARFRAE
                     TGFDYHALRGEEIAWLARLGMLEEEGGCLRLTEKGLAVSNAVFAELV"
     gene            9685..10665
                     /gene="amrS"
                     /locus_tag="PK634_05185"
     CDS             9685..10665
                     /gene="amrS"
                     /locus_tag="PK634_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOW97163.1"
                     /translation="MSGGLVQCELCPKGCVIGPGQSGDCRIRVNLDGRLCAVTYGFPV
                     SVHVDPMEKKPLFHFLPDTGILSLATAGCNLHCKNCQNWEISQRNPEEVEATPLPPED
                     IPPLARRTNCLSVAYTYTDPAAYYEYALDSCRLCREAGLRNVLVTAGYINPEPLRELC
                     RYVDAANIDLKAMSDSFYRTVCDGTLQPVLDALVLAKSLGVEVEVTNLVIPTLNDRDA
                     DLVPLARWVKEHLGAETPLHFSRFFPQYQMRHLPPTPEDTLLRARDLARAEGLKHVYV
                     GNVETRDGETTFCPDCGAELIRRDRYVILSNRLRDGRCPDCGREVYGVWK"
     gene            10662..10850
                     /locus_tag="PK634_05190"
     CDS             10662..10850
                     /locus_tag="PK634_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97164.1"
                     /translation="MKRRRFLQVVLAAAAALAAAGVGLLRRASPVRVVRAWRVRRYPG
                     PVRALDEARVRHPGPWTG"
     gene            10892..12427
                     /gene="amrB"
                     /locus_tag="PK634_05195"
     CDS             10892..12427
                     /gene="amrB"
                     /locus_tag="PK634_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOW97165.1"
                     /translation="MHLVRRGGLLPWVVLALGLTSCTRAETPPAAEKKVLATHLAGSW
                     FTADPDELARELQGYMDRAEGPVLENVVALIQPHAGYRFSGPAAGFGARQIAGRPFTR
                     VVVLGPSHRVPMRNLVALPDATHFSTPLGEVPLDLDFMRTLGSSPYFRVIPEAHAGEH
                     SVQIQVPFLQQALGDFRLVPLVVGQLDEAAIRGAAEVLRGLLDEATLVVASSDFTHYG
                     PNYGYLPFRDDVEKNLEKLDMGAVDLIRNKGVAGFQAYCGRTGATICGRDPISILLAL
                     LPADAEARLLRYDASGRVTGDFENSVSYVSLAFTGRWKKEEAMPKPASEFTLTADDQQ
                     ALLKLARGTIEYYLEHGRPPKPEDLGIAVSSTMQQVAGAFVTLHKRGDLRGCIGEIVP
                     RRPVCQAIVEQAVNAAVNDYRFNPVTADELPGLHVEISVLTPPVDVKSWRDIVIGRHG
                     MILSKQGRSAVFLPQVAPEQGWGIEETLTHLSMKAGLPPDAWREGASYKVFEAVVFGE
                     EKK"
     gene            12472..16245
                     /locus_tag="PK634_05200"
     CDS             12472..16245
                     /locus_tag="PK634_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOW97166.1"
                     /translation="MNRDVRVGFRLAVVFGLGFFALAAQSLLFREFFSVFEGNELGIG
                     VFFSSWLLWAGAGALAARPLGRRPRLTAAFDWLPLLYLPAYIAQWHLVHNARALAGVE
                     PYELFPLAKMLPAAMAANAPVSFVTGLLFTLACAWIADAGGLPPARVYFFEAFGGAAG
                     GTAVTAALATGWPSETVFLAAALLPAAAPLAGRRRAALLPLLLLVAALGLRADRAWRQ
                     RNDRASWSRVLPAASFRGRFVTPQATYLYGEYAGQFIVRAWESVAESIPDEERAGEIA
                     ALGLAQAPAARRILVVGGGYSLCERFLELAQVESVTWLHPDPDYPARLLGVLPAERHG
                     RDDRLRVPAADPRRFLADDETRYDLAVLNLPDTMTLSLNRYGTREFYRRLRDRLDPTA
                     GVAAIRFSGGENYLGGELVNLGASVLTTAESVFPTNGLQPGGDSWLFVSVGGELVDSP
                     AAARERFASIPGAGKVCLPERLLSAFSADRIEFQRTAYRQAAERSPAGWLLNTDARPR
                     ALLFALLLAGLQGGLAREAAAAIRALSVMGPWLFLLPWLLYGALRFAYRLRGRPAATS
                     GFDAGFLVFSAGLAGMSFSLVLMFLYQSAFGSIYLQIGLLSALFMLGLFAGGWTVERV
                     LVRLVREPAGLLPLAIALHVGLIACAWGAGGRPAFSALFFLAGFFGGAYVPVAAFRLK
                     RAGRDDAASGAAVEGLDTLGGAAGGLLLGLLILPLHGLAAGLAVTALALAVNLPAHAL
                     RARVSASTDAADRWARRLGYLMAGATILALFVSWMSDRARRTEPGDLLREAAGILVPG
                     QPFAARQAQLTDGRTVSYLEVQGGGFLLTTDQLAPDVSGYGGPITMGVLLDSEGTLQA
                     ARILRAQETPQYRKAAEEWLVTLRGRNLFDMASMREVDGASGATLTSTAVLETLRAAG
                     CLFSQQVLKINPAETARGARRIPFDAKAAVFLGLAVAALALRRRPWPWIRRLFLLVVA
                     ILLGWRWNMQYSAAHVLAAIAGAWPAAGVTIPFLLAAGVPALVAIFGNVYCGYLCPFG
                     ALQELAGEWRPQSWSFDPDRSAWRWARWLKYGLLLALVLGFALRRDLGLADADPLNAI
                     FSPLRSPLALSAGLAVLLLSVVYRRFWCRNLCPAGAFLALLNGLKLTVRWWPPVRPGC
                     CDYGVRHVRELDCILCDRCRTRAATAEARNPVLRAVVFYILLAGAVLYFAPRLRTRGE
                     PSVPAPAAAEPGALRTHRARDVDMPRLRALLDRGLLSTNEALYYRRPEDGAPE"
     gene            16284..17453
                     /locus_tag="PK634_05205"
     CDS             16284..17453
                     /locus_tag="PK634_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97167.1"
                     /translation="MHTAFRFDIRTLGVAVSAGLLAHSVLGVTINHANGATATNAALS
                     VMAKVGQLRWFFTHASVGGNITTGMNVLHGTDTNRYRLTIYNYDGDNSDGAYHGGVGT
                     EGSEGGEDYRAAADPEIVTNGIVYECMRGNPDWSNKLVCFSNSVAVSGWRFPRVNVVI
                     DKFCWIDPYADPTNYCALISALESQYPETLFVYLTMPLSGLNFDENDERNSFNRHVRS
                     FCSAHNKHLLDVADLEAWNLSGVQQTYLSSGVTNQKMSFDYSLDTGGDWHLNAAGRRR
                     MALGWYSLAAALFAADRDGDGITDGDELLAGTRAADSNSALQIRISPASFSSSSNAVF
                     TWTASSGRYYSVQRATGLVNGVTWSNRLADRLGANSFTDTVEAAVQGFYLLSVRQ"
     gene            complement(17459..19285)
                     /locus_tag="PK634_05210"
     CDS             complement(17459..19285)
                     /locus_tag="PK634_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HOW97168.1"
                     /translation="MKKIPTLLLGLAAGVAGFGIDPARALEPGFFYFGSNRVLSVYDG
                     LSGLLTGANGFRLAGSADESRLGSALSAAGDINGDGYGDIVVGAPFFNSPSFREGRAY
                     VLFGPGPALPSQQPTFLNGTNGFQVTGLAADDQLGASVAGLGDINGDGRADVAFGAPQ
                     ADPSGRTGAGAAYVVFGATNFTLNVSLAGLNGTNGFTLAGEAAGFQCASALAPAGDVN
                     GDGRADFLVGAEKATIGGQTNIGRVYLVYGATNYPSSLDLGALNGTNGMLLTGESASD
                     SAGHALAALCDYNGDGLDDILVGAHTAPDFFSAGRAYAIFGSTNRTASLSLAALNGTN
                     GFRIDGDGLFGNCGYAVAASDVNGDGLFDAAVSAHNLNEVYVLFGRADPPASLSVTNL
                     NGTNGFAVAYTNAEFGFGSALAGAARLNVDGYGDLLCGAPTFTADGRASAGEIFAIFG
                     RDVFPRELDPESLNGTNGFRLKGGQALAEAGGALASMSDMNSDTFDELLIGERLFDYS
                     IPGTNVVSTNAGAAYVVSPPGVAELVLYHPELIATGVSSGLYSVTWAGQTGVTYTVYT
                     NGSLAGTSWGEATNLVSGSATTTVERAAGSAPAVFYRISARR"
     gene            complement(19370..22456)
                     /locus_tag="PK634_05215"
     CDS             complement(19370..22456)
                     /locus_tag="PK634_05215"
                     /inference="COORDINATES: protein motif:HMM:NF028070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOW97169.1"
                     /translation="MRHLTRSILFSLGLCLAATSAMAFPTWIGTYGSFERHSNANPGT
                     FTILMNQDYVGLHAEVGVQVNGGSWTTHGMAYSGNADGNSKWTVTPAAAFPAGATVKF
                     YFHGWDDWGGHIYDNNNNQNYAYVVPGSSTGRVGWTTSAAIPANGALYMDIAARDGTI
                     YAVWAERADWNQPETVLFSKKVTGEGWTAPSQLASVSGGMTYPLIAASSRGLHVLYGD
                     NNQTRLVHSANGGTTWSAPIDVASDYAGPLSRYAQLRADDTYTYLAYDNFTAPETSRV
                     RFRRIHKDATAWEAERTIFDRTSYKATVQIKDFEVRGQKMIMSSSLVGWYGGFSTPYC
                     HESTDGGQTWTDRSTGQSYADGQLDADAAGHAYMAMVGGSLPEGLNLAHKDIGAAWGT
                     WTLFWPGGGTVDSFAKIDAGLVAVSTRNELRYYELSTDGGATWSAPALIEGSTSWINK
                     DVRDGNTVHLLARDNNAATYSVISSGPAGAPAVTWIGNTYHWPSGSELDATDDLWINI
                     ESTARGAAVSAGVAYSTDGVNWTNKAMELGGSAGANDWWHANLGKFAAGATVRYAVAV
                     TDGQGTTRWDNNGGQDYRATVASSVTVQTPVFWGLDPYRYDNAKARANGRAPQESNNS
                     FGQFDAGQAVTIVARPVENGNGNLVQTSCSSTALLHYTTTPGDWSNPQTVQGVFSPAG
                     LSNKPIFDFFSFDLGTFPAGTSVEFWMEAKNAAGTGYAQGAGNDFDFSIAAAVGDSDS
                     DGLPDQWELDYFGNLGQSATNNNSDLDGPTYFPIANIIEWVLGLSPNAPNDSMGIKLL
                     WSPAYPSPGEQVNLSYFYVNEGNPLFGKPIYAHVGHNGWQGVYDTAQFGLNGRISRFQ
                     ATITVPAGATEINVCFHDNAGTWDNNGGQNWRIPVQPAGGGGGGVAAGSATTSSKSAA
                     PAVEPAPAPKTLTVKWTSSRSGQWTLVAGQPGWKNVAQIPMRTAANGTRWATYTLPDG
                     ASQVVFAFHKSGQWETNNGRAWSVTLAGGGHHTLSIASARTATKVIHRTLKKSARK"
     gene            complement(22640..23215)
                     /locus_tag="PK634_05220"
     CDS             complement(22640..23215)
                     /locus_tag="PK634_05220"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOW97170.1"
                     /translation="MPNSQRLDFDAIVGFVTEGARVLDLGCGDGSLLERLVREKKAQV
                     RGVELSEDNVRACIARGLSVRHGNIEEGLADYRDGAFDFVILSQTLAYLDAPAPVVKE
                     MLRVGSAAVISFDNAGHWRCRCRALRGGGIGHTLVSGAPRERAITIPQFDDFTREIGA
                     HIRQAVHLTGRRPIARFRRLRATAAVYVISK"
     gene            complement(23278..24402)
                     /locus_tag="PK634_05225"
     CDS             complement(23278..24402)
                     /locus_tag="PK634_05225"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOW97171.1"
                     /translation="MNEIVKTQHATFDEPLKLQSGATLPAPFTMAYETYGTLNAGRSN
                     AILILHALSGDAHVAGRHSAADRKPGWWDDAVGPGRAFDTDKYFLICSNVIGGCQGST
                     GPSSVNPKTGEPFGLQFPVLTVADMVEAQRLLLDRLGIETLLSVVGGSMGGMQALQWT
                     ISHPARVRSAIVLAATAQVSPQSIAFNEVARQAIYADPKWKHGKYTKEEAPKEGLSVA
                     RMIGHITYLSDRSMREKFGRRLQERQKLGYDFATEFAVESYLKHQGDHFTQRFDANTF
                     LFITKAIDYFDLAHGRASLADAFQKVTARFLVVSYSSDWLYPPEQSEALVRALLQNGI
                     DVTYVEIHSDYGHDSFLIETDRLAGLSRDFLARVAAVKAP"
     gene            24604..25242
                     /locus_tag="PK634_05230"
     CDS             24604..25242
                     /locus_tag="PK634_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOW97172.1"
                     /translation="MAEELQHLIERIQKEGVEKADAQAAKIISQAKEKAAAHVREAEE
                     KAKTLVAKGEKDAQAFMERSIRSLEQAARDLLITVGQGVENILSDLVAESVDKAMTIQ
                     VLEQMLVKMAEAYAARGGEESRIELLIGPQDQQDIVKFFGDRYKEQLVRGVKLHVDND
                     IFKGFKVSFADGRVQHDFTREAIAESLVNFLRPQLAEIVHRVAREGSQTDKG"
     gene            25249..25788
                     /locus_tag="PK634_05235"
     CDS             25249..25788
                     /locus_tag="PK634_05235"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOW97173.1"
                     /translation="MKPYYLVASLPTLVLGDPAPLDVASFLAACANLLSEEELAEVTL
                     MLEGRVGESGAAFAKDWLRVDTQLRNAVARVRAGKRSAEARSFLREHEGYDVSLEKAV
                     TDAYTRPNPLERELFLDRHRWQRLDELVLAEPFGFAAVLAYALKLRLVLRWADLNDEA
                     GGQRLTELLKELDKSEGKT"
     gene            25806..25888
                     /locus_tag="PK634_05240"
     tRNA            25806..25888
                     /locus_tag="PK634_05240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25839..25841,aa:Leu,seq:taa)
     gene            26016..27809
                     /locus_tag="PK634_05245"
     CDS             26016..27809
                     /locus_tag="PK634_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOW97174.1"
                     /translation="MSENIGKIVGVNGNLLTVEFNQPVTQNEVGFAALGDLKLKSEVI
                     RIRGRRAELQVFEDTTGLKVGDPVEFTGDLLAVELGPGLLTQIYDGLQNPLPALAEKC
                     GFFLQRGAYLQSLDRQRKWAFTPSAKKGDTVQAGDKLGSVPEGIFEHRIMVPFGVRGK
                     CTVEDVAAQGEYRVEDVVAKIRNERGDVLEVRMMQRWPVKFPIRAYGERLRPVEPLIT
                     KVRIVDTFFPVARGGTYCIPGPFGAGKTVLQQITSRYADVDVVIIAACGERAGEVVET
                     LREFPELTDPRTGKSLMERTLIICNTSSMPVAAREASVYTAVTIAEYYRQMGLHVLLL
                     ADSTSRWAQALREVSGRLEEIPGEEAFPAYLESVIAAFYERGGLVKLRDGGVGSVTIG
                     GTVSPAGGNFEEPVTQSTLKVVGAFHGLSRERSDARRYPAIHPLESWSKYPSFVSDED
                     VREARHVLRQGNEVGQMMKVVGEEGTSLDDFVVYLKGEYVDAVYLQQDAYHAVDAATS
                     SARQQYVFERLVKILRTKMAFDDKDGARKFFQQLTQTTKDWNRAPMPGDDASPASGRR
                     ACHRRPSSVPVTTTSPSSESSAALTTIGSPA"
     gene            27864..>28079
                     /locus_tag="PK634_05250"
     CDS             27864..>28079
                     /locus_tag="PK634_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97175.1"
                     /translation="MSVSTGLSSCTSSAPIFCATSRTSPIIVWLPRPNCAALTMKGEF
                     GAEPNPTVNRRGSESRSRISVNSAASLP"
BASE COUNT         4319 a         9418 c         9720 g         4622 t
ORIGIN      
        1 gacgaggtcc tgccggacaa cgactgctgg ctggtggcct cccggtacgg ggcgctctcg
       61 acggtggacg gcgtaccgat gatcttctac gggcaggagc agggcatcca gaactacaac
      121 tcctcggacc ccggcggcac gtggtactac gacgggttcc gcgaggacca cgaggagaac
      181 ttcggcaagt acatcccgca tttcaagaaa tggaaccagc tcaccgtgtg gtcgaacccg
      241 ccgccgaaca gcggagggat cgcggagtgg tacgggaagg tgaaccgggc ccgccgcttg
      301 agcccggccc tgcgcagcca taatcgctac ttcctctcga aggtcggggg cggcgaggag
      361 gcgcgcatcc tcgccgtggc gaagtacgaa acggcgaacg cggggccggc cgcgtccgat
      421 gtcgtgctcg cgttttccct cctgctgcgg cacggggagg cgcataccgg ctccagcgcg
      481 acgtacaacc tgcagcccgt gtggaccctc ctcggcctga acacgggcaa gagctacaac
      541 gtccggaatc tggccgcgag caacccggac cagttgctga cgagcggctg gccgaaaacg
      601 ggcgcatctc tttacaatga cggcatatgg gttaacctcc cggccgacac gggcggggcc
      661 atcaccgatg acggcgccct ggtgcagtac ctcaagctgg aggaggtctc caccaacgcg
      721 gcgccctcga tcagcctgcc cggcccgcac tggctggccg tgggatcggc gaccaatttc
      781 agcgtgaccg cctccgacgg gaacggcaac gcggtgacgc tgacgaacac cgtgaagccg
      841 tcgggcgcga catttgccgc gggcgtgttc gcgtggaccg cgctgccggc ggaactggcg
      901 gggaccaccc aggccgtggt cttcgtcgcg gacgacgggc agggcgcgac gaacagcatc
      961 gtgaccaatg ccacgcagat cgtcgtgccg ttcgattccg acggcgacgg gatgccggat
     1021 ggctgggagt gggacagctt cgcctcgctg accaatccgc ccgcaggcga ctgggatggc
     1081 gacgagttga gcaacctgga cgagtacacc gcggggacga cgccgacgga cggcgattcc
     1141 atcttcgcgg tcgagacacc gaccccggca ggaacgatct ccgtccaggt gcccgttcgg
     1201 acggtcccgg ggcgcaagta caccatctac ttcgccgacg acctcggcgg ggattggagc
     1261 ccgttcgcca acccggcgca ggggatcggc acgtggacgg agaccggcgg ttcgccgtcg
     1321 gtgttctcct tcaccgatga cgggtccgcg gacaccacgg cgggacctcc gctcgagggc
     1381 cgccgcttct accgggtgaa ggtcgaaaag ccctgattgc tcccggcgcg cttccggcta
     1441 tgatggtcgg catgggcgag gaacgcgtca tggtccggtt gaaacccggc cgcgaaaaac
     1501 cggtgcgccg cgggcatccg tgggtttttt cgggggccgt ggattccatc gagggagccg
     1561 gggcgccggg cagcgtggcg gatgtggtgg cccatgatgg gacctggctc gcgcgcggcc
     1621 tgctgaaccc cggagctgcg ctgcaactcc gcatctacac gcgggataca gcgcaggcgc
     1681 tcgacgcgtc ctttttccag gcaaggcttg atgcggcgct ggctatgcgg gagcggctct
     1741 tccagttcgc cggcgggcgg acgaatgcct ggcggctcgt gttctccgaa gcggacgggc
     1801 tttccgggct catcgtggac cggtacgccg acgtgttgtc cgtccaggta ggcgccgcgg
     1861 ccctcgttcc gcacctgcag gccatcgtgg atcacctggc cgcgcgcacg ggcttggcga
     1921 tgcacctgcg gatcgaaccg gacgccgcga cccgcgaggc cgtggatgtc tccggcgtgg
     1981 cgacgccggg gccgtctgcg cccgggcggg tgatcatccg cgaaaacggg ttgtcgtttg
     2041 ccgtggacgt cgcgggaggg cagaagaccg gcttcttcct ggaccagcgc gagaaccgac
     2101 tccgcgtggc cgcctatgcc cgcgggcggc gcgtgttgag cgcctactgc tacaccgggg
     2161 ccttcgaggt gtatgccgct gcggccggag cggtttccat cgtcgggctc gatcgttccg
     2221 agcctgcgct ggaactggcc cgcgagcagc accggctcaa cgccacgacc atccctgtcg
     2281 agtaccgggc ggcggatgtg cccgaggcgc tgcggcgcta ccgcgacgcg ggcgagcggt
     2341 tcgacctcat catcctggat cctccgcgtt ttgtcgccag ccaggcgcaa ctggaaaaag
     2401 ggctgcgagc ctacaaggac atcaacctcc tggccctgaa actcctgacg ccgggcggca
     2461 tcctcgccac cttttcctgc tcgggactgg tatccgccga ggcgtttcgg acgatgctgg
     2521 gctgggccga aaccgatgcc ggccggcgcc tgcgcattgg cgagaggctg acccaaccgg
     2581 ccgatcatcc ggggctgacc agcttcgtcg aaggcgacta tctaaaaggc ctgatcggct
     2641 atgtcgaata ggggcgccgc tttcataaca tgcttattgg aaggaaatta gatatttgcc
     2701 cgtcgcgcat caggcggcga cagcgaagcg ggcgaattga tattgacatt atttctgaat
     2761 gatataacct ttaagtggta aggtagtgtc catggaaccg gcaatgagca agggttcagg
     2821 cagagacgta tcggattgct cggctgacca gtgggtccgc aatacccgcc ttttccgccg
     2881 ctgggagtgc atgaagcagg aaatccaact ccacaagtgg tacgagagcg agaaggccgg
     2941 acatgacatc gggtgggagc gggcctgcgt ggactggatg atccggttcg gccatcacca
     3001 ccctgaagac tccatcccct gaacggctgg ctcgctgtcc cctgatcgcg ttctgacttt
     3061 ctttttggcc ccttccgcgc taacgtcccg acttgagcgt ggcgccctcg agcccgctcg
     3121 gaggtcctat ggccaagcta taccgtgaca atccgcttcg gaagatcgcc tgggaggact
     3181 ttcataccct ggatgcggcg gagaagggcc cgttttttct ccgcgagggc cgtccctatc
     3241 atgccctgaa tgcccagcag ttcacccgcc cgattctgga cggtctgtgc aacctggcca
     3301 cgcaggtgcg tcggatcgcc aaaacccgtg gcggggtgtt gttcctccag gacctgttgt
     3361 gcgaaaagcg ggcgatgctc tacttcgccc agccttcgac ccgcacgttc ctgtcgttct
     3421 acgcggcctg ccagctgctc ggcctcaacc cggccgaggt gcgcgacacg acgacctcaa
     3481 gcgagatcaa gggcgagtcg ccggaggatt cgatccgcac gttcagttcc ttcttcgaca
     3541 tcatcatcat gcgccacccg gccggcggct tcgcggagcg catcgcctgg ctgctctcca
     3601 acaccgaccg gccggtcccc gtcgtcaacg ccggttcggg gaaggaccag catccgaccc
     3661 aggccctcct ggacatctac acgctccagc gcagcttcga gaagcgagag ggcatcgagc
     3721 ggaagaagat cgcgttcgtc ggcgacctcg cccgcggccg gaccgtgcgg tccctggcct
     3781 ggctgctgac cctgtatccg ggcgtgaagc agtatttcgt ggccccgccg tccctgcaga
     3841 tcggcccgga catcctggcg gcgctggacc gcgccggcat ggcctacgag ctgtcgtccg
     3901 acctgcaggc cgtgatcccc gaggcggatg cgatctacat gacgcgcatc caggacgagt
     3961 gggacacggc gcacgagagc cgcgacgtgg acaccaaccg gttccatttc cgcgtggccg
     4021 acctcgcccg gctccggccg gatgccgtga tcatgcaccc gctgccgcgt cggcgggaga
     4081 tcgagccggg cgtggacggc gacccgcggg ccgtttactg gcggcaggtc cgcaacggca
     4141 tgtggatccg cgccgcgctg atcctgaccc tcttcgagcg cgagcgcgag atcgagcgct
     4201 attaccagga tctgaccagt tgatttcctg aaccctccca tgctctccaa gcccaagtcc
     4261 gaccaactgg ttctgcgcat ggccaagctg ggcgtgcgcg aacacgacct ggacgagcag
     4321 ttcatccgcg gctcgggacc gggcgggcag aagatcaaca aaaccagttc caccgtgtgg
     4381 ttgcgccacc ggccgagcgg catcgagatc aagtgcgggc gggagcggtc gcaggttttg
     4441 aatcgctacc tggcgcgccg ggagctctgc gaccgcctgg aggagaaaat cctgggcgcg
     4501 aaaagcgcgc ggcagcaggc catcgagaag atccgccgcc agaaacgccg ccgctcgcgc
     4561 cgccagaagg agcggatgct ggccgacaag cggcatcact ccggtatcaa gcagagccgc
     4621 tcaagatcct tcctgtcgga ctcttgaaca gggcgttcag cccagccagc ggcggaggag
     4681 ataataggcc gcgtggttgg ccgccaggct gtagagcgcc gcgaagacat cgtcgatgac
     4741 gatcccgagc ccgccgcgca gccgctgcag ctggttcgcc ggcggaagct tgacgatgtc
     4801 gaagaaccgg ttgctgaaga acgcgaccgg caaaatccac caggcgctcc acgataccgg
     4861 caggccgatc agggagatcg ggaacgtcag gtattcatcc gcgacgatgc aatgcgggtc
     4921 ctttttcttg aaatgccgtt ccgcgacgtc gcagatcggg atcgccacca gggccaggcc
     4981 caggcacagc aggacctggc gcgccacggg cagggagggg aagagcagga ggacgatcag
     5041 gacgccccaa accgagccca cggtgcccgg cgcaaagggg ctgtagccca gccccagccc
     5101 ggtggccagc gcgacgcaga cgcggcgggc cagatccttc atcgctcgcc gcccagcttg
     5161 tagctcactt ccatgcgcgt gccgtccgat cgcatccaga accgggcgcg gctcaacggc
     5221 atcagcgagt ccacccaagc cttggcctcg ttgagcttct gccgcgtctc ctgcgagccg
     5281 ccgggatcct cgagcagcag cttgagcgaa taggtggaga gcgccatgcg cagcgtcttg
     5341 cccgcgcgcg ccaggtccag ccatacatac acgggggcgg gctcgttatc caggtcccgc
     5401 atccaggaca ccggcggcgg ccggttcgtg ggcagcgcgt cgaaccggtc ggccagtttc
     5461 tccaggccct cggaattgct ggccagcagg agccagccgt cccggagcat gtaggccggg
     5521 cattcggagg ggggcagacc cgccaggaag ttggtcgagg tcgcctcgat gatcgtcatc
     5581 cggccgcggc cggcggccac ttccctcggg accagccccc agcgatagcg ggcgttgagg
     5641 cggtccaggg cctcccgaag ccaggcggac acgagttcgc tcttgccgac ggggagcgcg
     5701 cccacgaggg tcggcagctt gatgcctttc aggcggccgc tgtagtcgtc cccgagaagg
     5761 gccagcgcca cgacgccggc tttttccagc cggatgatct ccgccatcag ccgcatccag
     5821 gccgcccgct tcggctgctc catgtagggc agcacgaggc tgggcgacag gagcgccagc
     5881 ccctgcggca ggtcgcccag caggcggtcg aggccgccgg ccttcagccg gggcgcccgg
     5941 gagaattcga cggcgaacgg gtcacggccg gtgacgcagc cgcgcaggcc gccggattgc
     6001 agttcgctcc actcgtagga taccgcgccg acgggcgacg tgacgccgcc cgacaccgcg
     6061 gcgggattca cccagccgcg gtccggggcc ttcggatcgc ggcaccagat gcccgtgcgg
     6121 cccgggaagt ccggctggtc ctccagcgac ggctgcacgc cgtccagcgc gtccagcgct
     6181 tcgttcagca ggcgcgggtc ggacgagaac acgccgacac ccatgccctc gacgagggcg
     6241 aacgtgagct tcggctcgtc ctccccgccc gtgtccaacg tccacgaggg ccagccgcgg
     6301 tacggcggat gggccgtcag ctccctgatc ttggcccagc gcaggttcca gcggagccgc
     6361 tggctcttgg ccccgagcca cgtgacgatg atccacgcgg gctcgccggg cgtgcccagc
     6421 gacggaacgt atgcgacaag ggcctcgtcg gaggccagtt gctgcagcca ctggcgcgcc
     6481 accgggtccg cggacagttc gttcagttct tccggcttca cgccgaacgc caggaacagg
     6541 gtctggatca gcggattgcg gtggatagtg tcccagcggc cggcgagatc gcggtggacg
     6601 ctgacaaagg ccgtctgcgc cggcagcgcg cggtacatgt gctccggccg gtagggcgtg
     6661 tagagcaccc accacgcggc ggcgcccagc gcggccgcga gcaggagcca gaaaagcaaa
     6721 gggcgtctgc gaatcatgag cgcaatcctc ccgtcacatc ccgcacgtgg cccagcccgt
     6781 gccgggccag atagtcgccg atcccctcgg cgatttcgat cgcggccgcc gggttcagga
     6841 acgtcgccgt gccgacggcc accgccgtcg cgcccgccag caggaactcg atcgcgtccg
     6901 ccgcgcacgc gatgccgccg atgccgatca ccgggatctt caccgcgcgc gccgcctccc
     6961 agaccatctt cagcgcgatc ggcttgatcg cgggcccgga caggccgccc gagacgttcc
     7021 ccagccgcgg ccggcgcgtc tcgatatcga tcgccatcgc cgggatcgag ttgatcagcg
     7081 agatcgcatc cgccccgcag ccctccgcgg cgcgcgcgta ctccgcgacc cgcgagaggt
     7141 ccggggccag cttgacgatc agcgggcggc gcgtcttcgc ccggacggcg gacacgacgc
     7201 gccggaacag cgccgggtcg ccgccgaaga gagcgctccc ttccttgatg ttcgggcagg
     7261 agacgttcac ctccaggccc gcgacgccct ccgcgccctc gaaaccggcg gcgacttcct
     7321 cgtactcctc caccgtccgc ccccagatat tcacgatcac cggcacgttg tacccgcgca
     7381 ggaaggggag gccgtcccgc aggaacccct cgagtcccgg gttctgcagg ccgatcgcgt
     7441 tgatcaggcc gccgacggtt tccaccgtgc gcgccggcgg gttgcccatc accggtttcg
     7501 gcgagatgcc cttcacgacg atcgcgccca ggcggttcag gtccaccagg tccgcgtact
     7561 ccggcccgta cccgaacgtg cccgacgcga ccatcaccgg attcctcatc cggatgccgc
     7621 cgatttccac tgacagatca atgcccattt ccctgaaacc ttactcgtcc tcccaaacaa
     7681 tctcccggct ctcgaacacc ggcccctcgc ggcatacccg ctgccaggtc cagccgccgt
     7741 ccggagcgtg gatcttctgc acgcaggtca ggcaggcgcc gacgccgcag cccatgtgcc
     7801 ggtccagcga gagccacgcc ttccagccgc ccgcgatggc ccgctcgccg atggcgcgca
     7861 gcatacccat cggtccgcag gcgaacagtt cgacgccttc gcgcggcggc ttcttcatcc
     7921 attcatccag cggcgccgtc accagcccgc gttcgccgag actcccgtcc tcggtggcga
     7981 tccggaccgg ccagccgagc gcctcgaact cgtccacgca gaggacgtcg tccgccgtct
     8041 tgccgccgaa gaacgcgatg ccgcgtcgcg gcagggcccg ggccagcagg tggagcgccg
     8101 ccatgccgta tccgcctgcc accaggacag gcatccggcc tccgcattcg gccgggaagc
     8161 cgcggccgag cgggccgatc aggctcacgg tctcgccggg cgccgcgccc ttcaggaccg
     8221 ctgtgccgtg gccggcgtcc ttgtaaagaa tggacagggc gccgtcccgg gcgtcgaaaa
     8281 cgctgaacgg ccttcggaga accgccgcct ccaggtgcgg gacgcgcagg tgtacgaact
     8341 ggcccgggcg cacctccggc gcgatgccgg gcgcccgcag caccagccgc ttgtatccgc
     8401 cctggatgtc gcgatgctcc aggatctggg cgttttccat tcgcatagga agccagagtg
     8461 tgcgtggttt tttccgggcg gtcaaacccg aagcatgcgg gttttccaat cattgaaaaa
     8521 aacggtaaac tttttttcca atcattggaa aacccatgcc cggcctctac attcacgtcc
     8581 cgttctgcgc ccgtaaatgc gcgtactgcg atttctactc cgtgccgctc aacgccgccg
     8641 cggcagagga gtatctgcgg gcgctggacc gggagtgcgg cgggctgccg gacggcttcg
     8701 cgccggagac ggtgttcatc ggcggcggta cgccgaccgt gctcccgccg gcggaattgg
     8761 cgcggttgct cgacctgctc gcacggcggg tgaacctcgg ccgcgtgacc gaatggacct
     8821 gcgaggccaa cccggtgacg ctggacgcgg aaaagatccg gatccttcgc ggcgcgggag
     8881 tgaaccgcgt ctcgctcggc gtgcagtctt tcgacgacgg cgtgttgaag ttcctgggcc
     8941 gcctgcacac cgcccgcgag gcggtcgagg cttttcgcct gctgcgcgcg gagggcttcg
     9001 acaacatcgg cctggacctg atgtgcgccg tcccgggcgg cgcgatcgaa cgcgacctgg
     9061 agcgggcgct cgcgctcggc ccggagcatt tgtccgtgta cgggctgacg ttcgaggacg
     9121 gcaccccgct ggcgcggcgg cgcgacgcgg gggaggtcgc cgagttggat gaagacgagc
     9181 agctccgcca gttccgcgtc gtgcgcgaga cgctgaaggg cgcgggcttt catcattacg
     9241 agatatccaa ctacgcgcgg ccggggcgcg agtgccggca taacctgctg tactggggac
     9301 cgggtgaata cctcgggctg ggcccggcgg cgcattcgca ttggggcggg cagcggtagg
     9361 gcaatccgcg ccgtctggag gactgggccg ccggcgcgcc gcgcgaattc gacgagacgc
     9421 tcgatccgga ggcgcgggcg cgcgaggcgc tggtgttcgg tctgcgccgg ctcgacggcg
     9481 tgggccgggc gcggttccgg gcggagaccg gtttcgacta ccacgcgctg cgcggcgagg
     9541 agatcgcctg gctcgcgcgg ctgggcatgc tggaggaaga gggcggctgc ctgcgcctga
     9601 cggagaaggg gttggcggtc agcaacgccg tcttcgcgga gctggtttga caggtccggc
     9661 gcgcgggcgg acaatgaacg cagcatgagc ggcggactgg tccagtgcga gctatgcccc
     9721 aagggctgcg tgatcgggcc cggccagagc ggggattgcc gcatccgcgt caacctggac
     9781 gggcggcttt gcgcggtgac gtacggtttt cccgtttccg tgcacgtgga cccgatggag
     9841 aagaagccgc tgttccactt cctgccggac acgggcatcc tgtccctcgc caccgccggc
     9901 tgcaacctgc actgcaagaa ctgccagaac tgggaaatct cgcagcgcaa tcccgaggag
     9961 gtcgaggcga cgcccctgcc gcccgaagac atcccgcccc ttgcgcgacg gacgaactgc
    10021 ctctcggtgg cgtacaccta caccgatccg gcggcctact acgagtacgc gctggacagc
    10081 tgccggctct gccgcgaggc gggcctgcgc aacgtgctcg tcacggccgg ctacatcaac
    10141 ccggagccgc tgcgcgagct gtgccgctac gtcgacgccg cgaacatcga cctcaaggcg
    10201 atgtcggaca gcttttaccg gaccgtctgc gacggcacgc tccagcccgt gctggatgcg
    10261 ctcgtgctgg ccaagtcgct cggcgtggaa gtcgaggtca ccaacctggt catcccgacg
    10321 ctcaacgacc gcgacgcgga cctggttcct ctggcccggt gggtgaagga gcatctcggc
    10381 gcggagacgc cgctgcattt ctcgcggttt ttcccgcaat accagatgcg gcaccttccg
    10441 ccgacgccgg aggacaccct cctgcgcgcc cgcgatctgg cccgggcgga gggcctgaag
    10501 catgtgtacg tcggcaacgt ggagacccgc gacggcgaga ccacgttctg tccggactgc
    10561 ggggcagaac tgatccggcg cgaccggtac gtgatcctgt ccaaccggtt gcgcgacggg
    10621 cgctgccccg attgcgggcg ggaggtgtac ggcgtatgga aatgaagagg cgccgattcc
    10681 tccaggtggt gctggccgcg gcggccgcgc tggcggcggc cggtgtcggc ttgctccgcc
    10741 gcgcgtcgcc cgtccgcgtg gtgcgggcct ggcgcgtgcg gcgctatccg ggtcccgtgc
    10801 gcgcgctgga cgaggcgcgc gtccggcacc cgggcccgtg gacgggttga tgaccgggcc
    10861 cccgtttgat acccttaaca aaggagtgct catgcatctt gttcgacgag gcggcctgct
    10921 gccgtgggtt gtattggctc tgggccttac ttcctgcacc cgcgcggaga cgcccccggc
    10981 ggcggagaaa aaggtcctgg ccacgcatct ggccggatcg tggttcaccg ccgacccgga
    11041 cgaactcgcg cgcgaactgc agggctatat ggaccgcgcc gaggggcccg tcctcgaaaa
    11101 cgtggtggcg ctgatccagc cccacgccgg gtaccggttt tccgggccgg ccgccgggtt
    11161 cggggcgcgg cagatcgccg gccggccgtt cacgcgcgtg gtggtgctgg gccccagcca
    11221 ccgcgtgccc atgcgcaacc tcgtggcgct gcccgacgcg acccatttct ccacgccgct
    11281 gggcgaggtg ccgctggacc tggatttcat gcggacgctg ggctcgtccc cgtatttccg
    11341 cgtcatcccg gaggcgcacg ccggcgaaca cagcgtgcag atacaggtgc cgtttctgca
    11401 gcaggccctg ggcgatttcc gcctggtgcc gctggtcgtg gggcagttgg acgaagccgc
    11461 catccgcggc gcggcggagg tcctgcgcgg tctcttggac gaggctacgc tggtcgtggc
    11521 gagctccgat ttcacgcatt acggccccaa ctacgggtac ctgccgtttc gcgatgacgt
    11581 cgaaaagaat ctcgagaaac tggacatggg cgcggtggac ctcatccgga acaagggcgt
    11641 ggcgggcttc caggcctact gcggccggac cggggcgacg atctgcggcc gggacccgat
    11701 ctccatcctg ctggccctgc tgccggcgga cgccgaggcg cgtctcctgc gctacgatgc
    11761 ttccggccgg gtcaccggcg atttcgagaa ttccgttagc tacgtcagcc tggccttcac
    11821 cggccgctgg aagaaggagg aagccatgcc caagcccgcg tccgaattca cgctgacggc
    11881 cgatgaccag caggcgctgc tcaaactggc ccgcggcacg atcgagtatt acctcgaaca
    11941 cggccgtccg ccgaaacccg aggacctggg aatcgcggtc agctccacca tgcagcaggt
    12001 cgccggcgcg ttcgtcacgc tgcacaagcg gggcgacctg cggggctgca tcggcgagat
    12061 agtgccccgc cggccggtct gccaggccat cgtcgaacag gcggtcaacg ccgccgtcaa
    12121 cgactaccgc ttcaaccccg tcaccgccga cgagttgccg ggcctgcacg tcgaaatatc
    12181 cgtgctgacc ccgccggtgg acgtgaaatc ctggcgcgac atcgtgatcg gccggcacgg
    12241 catgatcctc tccaagcagg gccggtccgc ggtattcctg ccgcaggtcg ctccggagca
    12301 ggggtggggg atcgaggaga cgctgaccca tctctccatg aaggccggcc tgccgcctga
    12361 tgcctggcgc gagggcgcga gttacaaggt gttcgaggcc gtcgtgttcg gcgaggaaaa
    12421 gaaatagttc cgaccgcgga gggcggcgcg ccgtcgccaa tgccaagtgg aatgaatcgc
    12481 gatgtccgtg taggatttcg tctggccgtg gtgtttggtc tgggcttctt cgccctcgcc
    12541 gcgcaatcgc ttctcttccg cgagttcttt tccgtgttcg aggggaacga actcggcatc
    12601 ggcgtgttct tcagttcgtg gcttctgtgg gccggcgcgg gggcgctcgc ggcacggccg
    12661 cttggccggc ggccgcggct gaccgcggcg ttcgactggc ttccgctgct ttatctcccg
    12721 gcctacatcg cccagtggca cctggtgcac aacgcgcggg cgctggccgg cgttgaaccc
    12781 tacgagctct tccccctcgc gaagatgctg cccgcggcca tggcggcgaa cgcgccggtc
    12841 agtttcgtga ccggactgct cttcacgctc gcgtgcgcct ggatcgcgga cgcgggcgga
    12901 ctgccgccgg cccgggtcta tttcttcgag gcgttcggcg gggcggccgg cgggacggcg
    12961 gtcacggccg ccctcgcaac gggctggccg tcggaaacgg tgttcctcgc ggcggccctg
    13021 ctgccggctg cggcgccgct ggcgggacgg aggcgagcgg cgcttctgcc cctcctcctg
    13081 ctcgtggctg ccctcgggtt gcgggcggac cgggcctggc ggcagcggaa cgaccgggcg
    13141 agctggtcgc gtgtgcttcc cgccgcttcg ttccgcggcc ggttcgtcac cccgcaggcg
    13201 acctacctct acggagaata cgccgggcag ttcatcgtcc gcgcctggga gtccgtcgcg
    13261 gaaagcatcc cggacgagga gcgcgccggc gagatcgccg cgctgggcct ggcccaggcg
    13321 ccggccgcga gacggatcct ggtcgtgggc ggcggctact cgctctgtga gcggtttttg
    13381 gaattggcgc aggtcgaatc ggtgacgtgg ctccatcccg atcccgacta tcccgcccgg
    13441 ctgctcggcg tcctgcccgc ggaacggcat ggccgggatg accggctgcg ggtgcccgcg
    13501 gccgacccgc gccgcttcct cgcggacgac gaaacccggt acgacctggc ggtgctgaac
    13561 ctgcccgaca caatgacgct ctccctgaac cggtacggca cgcgggagtt ctaccggcgg
    13621 ctccgcgacc ggctcgatcc gacggccggc gtggcggcga ttcgtttttc aggcggggaa
    13681 aattacctgg gaggcgaact ggtcaacctg ggcgcctcgg tgctgaccac ggcggagtcg
    13741 gtattcccga cgaatggcct gcagcccggc ggcgatagct ggctgtttgt ctcggtcggc
    13801 ggcgaactcg tcgactcgcc cgcggccgcg cgggaacggt ttgcgtctat tccgggggcc
    13861 gggaaggtgt gccttcccga gcggctgctc tcggcgttct ccgcggaccg gatcgagttc
    13921 cagcggaccg cctaccggca ggccgcggag cgatcgccgg cggggtggct gttgaacacc
    13981 gacgcgcggc cccgggctct gctcttcgcc ctgttgttgg ccggactgca gggcgggctg
    14041 gcccgggagg ccgccgcggc aatccgcgcg ctgtcggtca tggggccgtg gctgtttctg
    14101 ctgccgtggc tgttgtacgg cgcgctccgt ttcgcgtacc ggctgcgggg caggccggcg
    14161 gcgacatccg gcttcgacgc gggctttctc gtgttctcgg cgggcctggc cgggatgtcg
    14221 ttcagcctgg tcctgatgtt cctctaccag tcggccttcg gttcgatcta cctgcagatc
    14281 gggctgctct ccgcgttgtt catgctggga ttgttcgcgg gcggctggac cgtcgagcgt
    14341 gtgcttgtgc ggcttgttcg agaacccgcc ggcttgctgc ccctggccat cgcgctgcac
    14401 gtcgggctga tcgcctgcgc gtggggcgcc gggggccggc ctgcattttc ggccctgttc
    14461 ttcctcgcgg gtttcttcgg aggcgcctat gtcccggtcg ccgcattccg gttgaagcgc
    14521 gcgggacgcg acgacgccgc ttccggcgcg gcggtggaag ggctggatac gctgggcggc
    14581 gcggcgggcg gcttgctgct cggtctcctg atcctgccgt tgcatggcct cgccgccggc
    14641 ctggccgtga ccgcgctggc gctggccgtc aacctgccgg cccatgcgct ccgggcccgc
    14701 gtttccgcgt cgaccgatgc ggccgaccgc tgggcgcggc ggctgggata tctcatggcg
    14761 ggcgcaacga tcctcgcgct gttcgtgtcc tggatgtccg atcgcgcccg gcggaccgag
    14821 cccggcgacc tgctgcgcga ggccgccggc atcctcgtgc ccggccagcc tttcgccgcc
    14881 cggcaggccc aactcacgga tgggcgaacc gtgtcctatc tcgaggtgca aggcgggggc
    14941 tttctgctca ctaccgacca actggcgccg gatgtgtccg ggtatggcgg cccgatcacg
    15001 atgggcgtgc tgctggattc ggaggggacg ctgcaggccg cccggattct ccgcgcgcag
    15061 gaaacgcccc agtaccggaa ggccgcggag gaatggctcg tgaccctgcg cgggcggaac
    15121 ctgttcgaca tggcctccat gcgggaggtg gatggcgcga gcggggcgac cctgacctcg
    15181 acggccgtgc tcgaaacgct gcgcgccgcc ggctgcctgt tttcgcagca ggtccttaaa
    15241 ataaatcccg ccgaaacggc gcggggcgcc cgccgcattc cctttgatgc gaaggccgcc
    15301 gtctttctcg gcctggccgt cgcggccctc gcgctgcggc gccgtccgtg gccgtggatt
    15361 cgccgcctct tcctgctggt tgtggcgatc ctgctcggct ggcgatggaa catgcagtac
    15421 tcggccgcgc atgtcctcgc ggcgatcgcg ggggcttggc cggcggccgg cgtgacgatc
    15481 ccgttcctgc tcgccgccgg cgtgcccgcg ctggtcgcga ttttcggcaa cgtctactgc
    15541 ggctacctat gcccgttcgg cgcgctgcag gaactggccg gcgagtggcg gccccaaagc
    15601 tggagcttcg atcccgacag gtccgcctgg cgatgggcgc ggtggctgaa gtacggcttg
    15661 ctcctggccc tcgtgctcgg ctttgccctg cgccgcgatc tcggcctggc ggatgcggat
    15721 ccgctgaacg ccatcttcag cccgttgcgg agccccctgg cgctctccgc aggcctggcc
    15781 gtcctgcttc tgtccgtggt ctatcgccgg ttctggtgcc ggaacctctg cccggccggc
    15841 gctttcctgg cgctgctcaa cgggctgaag ctcaccgtgc gctggtggcc gccggtgcgg
    15901 cccggctgct gcgactacgg ggtgcggcat gtccgcgagc tggattgcat cctatgcgac
    15961 cggtgccgca cgcgggccgc gaccgcggaa gcgcgaaatc ccgttctccg cgccgtggtc
    16021 ttctatatcc ttctcgcggg cgccgtcctg tatttcgcgc cccggctgcg gacgcgcggg
    16081 gagccgtccg tccccgcgcc tgccgcggcc gaacccggcg ccctccggac gcatcgggcc
    16141 cgcgacgtgg acatgccgcg gctgcgcgcg ctcctggatc gcggcctgct ctccacgaac
    16201 gaagcgctgt attaccgccg cccggaggac ggcgccccgg agtaatctgc acaagccggc
    16261 cggcgcgggg tacccttgcg gtcatgcaca ccgcttttcg cttcgacatc cggacgcttg
    16321 gagtggctgt ttccgccggc ctgctcgccc attccgttct cggcgtcacg atcaatcacg
    16381 ccaacggcgc cacggccacg aacgccgcgc tgtccgtgat ggccaaggtg gggcagttgc
    16441 gctggttctt cacccacgcg tccgtcggcg gcaacatcac caccgggatg aacgtcctgc
    16501 acgggaccga caccaaccgc tatcggctca ccatttacaa ctacgacggc gacaacagcg
    16561 acggggccta ccacgggggc gtgggcaccg aggggtccga gggcggcgag gactaccggg
    16621 ccgccgccga tccggagatc gtgaccaacg ggatcgtcta cgagtgcatg cgcggcaacc
    16681 cggactggtc caacaagctg gtctgcttca gcaactccgt ggccgtttca ggctggcgat
    16741 tccccagggt caacgtggtc atcgacaagt tctgctggat cgatccgtat gccgacccga
    16801 cgaactactg cgccctgatc agcgcgctgg aatcccaata ccccgagacg ctcttcgtct
    16861 acctgaccat gccgctctcc gggctgaact tcgacgagaa cgacgagcgg aactccttca
    16921 accggcacgt gcgctcgttc tgctcggccc acaacaagca tctcctggac gtcgccgacc
    16981 tcgaggcctg gaacctgtcg ggcgtccagc aaacgtacct gtcctccggc gtgaccaatc
    17041 agaagatgtc gttcgactac tcgctcgaca ccggcgggga ctggcatctc aacgcggccg
    17101 gccggcggcg gatggcgctg ggctggtact cgttggccgc cgccctgttc gcggccgacc
    17161 gcgacggcga cgggatcacc gacggcgatg aactgctggc cggcacccgg gccgccgact
    17221 ccaattccgc gctgcagatc cgcatcagcc ccgcctcgtt ctcctcctcc tccaacgcgg
    17281 tgttcacgtg gacggcctcc tcgggccggt actacagcgt gcagcgcgcc acgggcttgg
    17341 tgaatggcgt gacctggtcg aatcgcctgg ccgaccgctt gggcgcgaat tccttcaccg
    17401 acaccgtgga agcagcggtc cagggcttct acctgctttc agtccggcag taagcggtct
    17461 agcgccgtgc gctgatgcgg tagaatacgg cgggcgccga gccggcggcg cgctcgaccg
    17521 tcgtggtggc cgaccccgac acgaggttgg tggcctcgcc ccacgaggtc ccggccaggg
    17581 agccgttggt gtataccgtg taggtgacgc ccgtctggcc ggcccaggtc acggagtaca
    17641 ggccgctgga gacgcccgtg gcgatcaact ccggatggta cagcaccagc tcggccacgc
    17701 ccggcggcga gacgacgtag gcggcgccgg cgttggtgga gaccacgttg gtccccggga
    17761 tgctgtagtc gaacagccgc tcgccgatca gcagttcgtc gaacgtgtcg ctgttcatgt
    17821 cgctcatcga ggccagcgcg ccgccggcct cggcgagggc ctgcccgccc ttcaggcgga
    17881 acccgttcgt gccgttgagc gattcgggat ccagttcgcg agggaagacg tcccgcccga
    17941 agatcgcgaa gatttccccg gcgctcgcgc gcccgtccgc cgtgaacgtg ggggcgccgc
    18001 acaggaggtc gccgtagccg tccacgttca gccgggcggc cccggcgagc gccgagccga
    18061 atccgaactc ggcgttggtg taggccacgg cgaatccgtt ggtgccgttc aggttcgtga
    18121 cggacaggct ggccggcggg tcggcgcgcc cgaagaggac atagacctcg ttcaggttgt
    18181 gcgcgctgac ggccgcgtcg aacaggccgt cgccgttcac gtcggaggcg gccacggcat
    18241 acccgcagtt gccgaacaac ccgtccccgt cgatccggaa tccgttggtg ccgttgagcg
    18301 cggccaggga cagggaggcg gtccggttcg tgctgccgaa gatcgcgtag gcgcggccgg
    18361 cggagaagaa gtcgggcgcc gtgtgcgcgc cgaccaggat gtcatccagg ccgtcgccgt
    18421 tgtaatcgca cagggccgcc agggcgtggc ccgcgctgtc ggaggcgctc tccccggtca
    18481 ggagcatgcc gttcgtgccg ttgagcgcgc cgaggtcgag cgaggacggg taattcgtcg
    18541 cgccgtaaac caggtagacg cgcccgatgt tggtctgccc gccgatggtg gccttctcgg
    18601 ccccgacgag gaagtcggcc cggccgtcgc cgttgacgtc gccggccggg gcgagggcgg
    18661 aggcgcactg gaagcccgcg gcctcgcccg ccagcgtgaa gccgttggtg ccgttgaggc
    18721 cggccaggct gacgttcaag gtgaaattcg tggcgccgaa gacgacgtat gccgcgccgg
    18781 cgccggtccg gccggagggg tcggcctgcg gcgcgccgaa ggccacgtcc gcgcgtccgt
    18841 cgccgttgat gtccccgagc cccgcgacgc tcgcgccgag ctggtcgtcg gcggccaggc
    18901 cggtgacctg gaatccgttg gtcccgttga ggaacgtcgg ctgctgggag ggcagggcgg
    18961 ggcccggccc gaacaggaca taggcgcggc cctcgcggaa gctcggcgag ttgaagaacg
    19021 gcgctccgac gacgatgtcg ccgtacccgt cgccgttgat atccccggcc gccgacaggg
    19081 cgctcccgag ccggctttca tccgccgagc cggccaggcg gaagccgttg gctcccgtca
    19141 gcagtccgct caacccgtcg tagacggaca ggaccctatt gctgccgaag taaaaaaagc
    19201 cgggttcgag cgcgcgggcg ggatcgatgc cgaatccggc gacgccggcc gccaggccca
    19261 gcaggagggt cgggatcttt ttcatggcgc gatactcaac aacggtccgt ttaaaaaagc
    19321 aaggcgggag cgcggtggag gacgcgctcc cgcccatcgg atgaaatgcc tatttacgtg
    19381 cagacttctt cagcgtgcgg tggatcactt tcgttgcggt ccgggcgctc gcgatggaca
    19441 gcgtgtgatg gcctccgccg gccagcgtga cgctccaggc ccggccgttg ttcgtctccc
    19501 actggccgct cttgtggaag gcgaagacga cctgggaggc gccgtccggc agggtatagg
    19561 tcgcccagcg ggtcccgttc gcggccgtcc gcatcgggat ctgcgcgacg ttcttccagc
    19621 cgggctggcc ggccaccagc gtccactggc cggagcggga actggtccat ttgaccgtca
    19681 gggtcttcgg cgccggggcc ggctccaccg cgggggccgc cgacttggag ctggtggtcg
    19741 ccgatccggc ggcgacgccg ccgcccccgc cgccggcggg ctgaaccggg atgcgccagt
    19801 tctggccccc gttgttgtcc caggtcccgg cattgtcatg gaagcacacg ttgatttccg
    19861 tggcgcccgc ggggacggtg atcgtggcct ggaaccggct gatgcggccg ttcaagccga
    19921 actgcgcagt gtcgtacacg ccctgccagc cgttgtgccc gacgtgcgcg tagatcggtt
    19981 tgccgaagag gggattgccc tcgttgacgt agaagtagga caggttcacc tgctcgcccg
    20041 ggctcggata ggccggcgac cacaggagct tgatgcccat cgaatcgttg ggcgcgttcg
    20101 ggctcaagcc gagcacccac tcgatgatgt tggcgatggg gaagtaggtc gggccgtcga
    20161 gatccgagtt gttgttggtc gcgctctggc cgaggttgcc gaagtagtcg agttcccact
    20221 ggtccggcag gccgtcgctg tcgctgtcgc cgacggcggc ggcgatggag aagtcgaagt
    20281 cgtttccggc gccctgcgcg taccccgtgc cggcggcgtt cttcgcctcc atccagaact
    20341 cgaccgacgt ccccgcgggg aacgtgccga ggtcgaacga gaagaagtcg aagatcggct
    20401 tgttgctcaa gccggccggg ctgaagacgc cctgcacggt ctgcgggttg ctccagtcgc
    20461 ccggcgtcgt ggtgtagtgg agcagggcgg tgctgctgca gctggtctgg accaggttgc
    20521 cgttcccgtt ctcgaccggc cgcgcgacga tggtcacggc ctggccggcg tcgaactggc
    20581 cgaacgagtt gttcgactcc tgcggcgccc ggccgttcgc gcgggctttg gcgttgtcgt
    20641 agcggtacgg gtcgaggccc cagaagaccg gcgtctgcac ggtcacggaa ctcgccacgg
    20701 tggcccggta gtcctgtccg ccgttgttgt cccagcgcgt cgtgccctgg ccgtcggtga
    20761 cggccacggc gtaacggacc gtggcgccgg ccgcgaactt gccgaggttg gcgtgccacc
    20821 agtcgttggc gcccgcgctg ccgccgagct ccatggcttt gttggtccag tttaccccgt
    20881 ccgtcgaata ggcgacaccg gccgagaccg ccgcaccgcg ggccgtggac tcgatgttga
    20941 tccagaggtc gtcggtggcg tccagttcgc tgcccgaggg ccagtggtag gtattgccga
    21001 tccaggtcac ggcgggcgcg cctgcggggc cggaggaaat gaccgagtag gtcgctgcgt
    21061 tgttgtcgcg cgccagcagg tgcacggtgt tcccgtcgcg cacgtccttg ttgatccacg
    21121 aggtgctgcc ttcgatcagc gcgggggcgc tccaggtcgc gccgccgtcc gtggaaagct
    21181 cgtagtagcg cagctcgttg cgcgtggaca ccgcgaccag gccggcgtcg atcttcgcga
    21241 aggaatccac ggtgccgccg cccggccaga acagggtcca ggtgccccag gccgcgccga
    21301 tatccttatg ggccaggttc aggccctccg gcagcgagcc gccgaccatg gccatgtacg
    21361 cgtggcccgc ggcatccgca tccaactgcc cgtccgcgta ggattgcccg gtggaccggt
    21421 ccgtccaggt ctgcccgccg tccgtgctct cgtggcagta gggcgtggaa aagccgccgt
    21481 accagccgac gagggaggaa ctcatgatca tcttctggcc gcgcacctcg aagtccttaa
    21541 tctgtaccgt ggccttatag ctcgtgcgat caaagatcgt ccgctcggcc tcccacgccg
    21601 ttgcgtcctt gtggatgcgg cggaaccgga cgcgggacgt ttccggtgcg gtgaagttgt
    21661 cgtaggccag gtacgtgtag gtgtcgtccg cgcggagctg cgcgtagcgg ctcaaggggc
    21721 cggcgtaatc gctggcgacg tcgatcgggg cgctccaggt cgtgccgccg ttggcgctgt
    21781 ggaccaggcg cgtctggttg ttgtcgccgt agagaacgtg aaggccgcgg gaggaggccg
    21841 cgatgagcgg gtaggtcatc ccgccgctga cggacgccaa ctgcgagggg gcggtccagc
    21901 cctcaccggt aaccttcttg gagaaaagga cggtctcggg ctggttccag tccgcgcgct
    21961 cggcccacac cgcgtagatc gtgccgtcgc gcgcggcgat gtccatgtac agcgcgccat
    22021 tggccgggat ggcggcggag gtcgtccagc cgacgcggcc cgtgctgctg ccgggcacga
    22081 cataggcgta gttctgattg ttgttgttgt cgtagatgtg cccgccccag tcgtcccatc
    22141 cgtggaagta gaacttcacc gtcgcgccgg ccgggaacgc ggcggcgggg gtgaccgtcc
    22201 acttcgagtt gccgtcggcg ttgccgctgt aggccatgcc atgcgtggtc cagcttccgc
    22261 cgttgacctg gacgccgacc tcggcgtgga ggccgacgta atcctggttc atcaggatcg
    22321 tgaacgtgcc gggattggcg ttcgagtggc gctcgaacga gccgtaggtg ccgatccagg
    22381 tggggaaggc catcgcgctg gtggcggcga ggcacaggcc caacgagaag aggatggaac
    22441 gagtcaggtg gcgcatggac cgtctccttg cattcctagt tgctcttgat gctccctact
    22501 cccgcgaaat tggcccatac agatacactc gcgcgggatg caagtaaagc gttttaccag
    22561 aaaaaatgat gatattgaga aaaagcctgt aaacacggat taaacaggca atttttttct
    22621 ggaaaggctg cgaaaaacac tacttggaaa tgacgtaaac agccgccgtc gcgcgcagcc
    22681 ggcggaaccg ggcaattggg cgccgacccg tcaggtgcac ggcctggcgg atgtgcgctc
    22741 cgatctcgcg cgtgaagtcg tcgaactgcg gaatggtgat ggcccgttcc cggggcgcac
    22801 ccgagaccag cgtatgcccg atgcccccgc ctcgcaaggc gcggcaccgg cagcgccagt
    22861 gcccggcgtt gtcgaacgag atgacggccg ccgatccgac gcggagcatt tccttcacaa
    22921 ccggcgcggg cgcgtcgaga taggcgaggg tctggctcaa gatcacgaag tcgaacgcgc
    22981 cgtcccggta gtccgcgagc ccttcctcga tgttgccgtg ccggacggac aggccgcgcg
    23041 cgatgcaggc gcggacgttg tcctcgctca actcgacgcc gcggacctgc gccttcttct
    23101 cgcggacgag gcgctccagc aggctgccgt cgccgcagcc gagatcgagc acgcgggccc
    23161 cctcggtcac gaagccgacg atggcgtcga agtccaatct ctggctgttc ggcatgggag
    23221 aatccttcct gcgctgcgga tacattcgcg cacggcgcgc ggaaggtcaa gcgcgggtca
    23281 gggggccttc accgcggcca cccgcgccag gaaatcgcgc gacagcccgg ccaggcggtc
    23341 cgtctcgatc aggaaactgt cgtggccata gtcgctgtgg atttcgacgt acgtcacgtc
    23401 gatgccgttc tggagcaggg cgcggaccag cgcctcggac tgctccggcg ggtacagcca
    23461 gtcggaggag taactgacga cgaggaaccg cgccgtgacc ttctggaacg cgtcggcgag
    23521 cgaggcgcgg ccgtgggcca ggtcgaagta gtcgatcgcc ttcgtgatga acaggaaggt
    23581 attggcgtcg aagcgctggg tgaagtggtc gccctggtgt ttgagatagc tctcgaccgc
    23641 gaactccgtg gcgaagtcgt agcccagctt ctggcgctcc tgcagcctcc gcccgaactt
    23701 ttccctcatg gaccggtcgc tcaagtacgt gatgtgcccg atcatccggg cgacggacag
    23761 gccttccttc ggcgcttcct ctttcgtgta ctttccgtgc ttccacttgg gatcggcgta
    23821 gattgcctgg cgcgcgacct cgttgaacgc gatgctctgc ggcgacacct gggccgtggc
    23881 cgcgaggacg atcgcgctcc ggacgcgcgc gggatgggag atggtccatt gcagggcctg
    23941 catcccgccc atgctgccgc cgacgacgga gagcagcgtt tcgatcccga ggcggtcgag
    24001 caggagccgc tgcgcctcga ccatgtccgc gacggtcagc accgggaact ggaggccgaa
    24061 gggctcgccg gtcttcgggt tgacggagga ggggccggtg ctgccctggc agccgccgat
    24121 gacgttggag cagatcagga agtacttgtc cgtgtcgaac gcgcggccgg gcccgaccgc
    24181 gtcatcccac cagccgggtt tccggtccgc cgcggagtgt cgcccggcga cgtgcgcgtc
    24241 gccggagagg gcgtggagga tcaggatcgc attgctccgg ccggcattga gcgtcccgta
    24301 ggtttcatag gccatcgtga acggggcggg cagcgtcgcc ccgctctgca gcttgagcgg
    24361 ttcatcgaac gtcgcatgct gtgttttgac gatttcgttc accgggtcct ctggcgcgtt
    24421 gtttcgcggc accatagcac agggccgggg gcgattccat agcggaaagc ctgtcacggg
    24481 ccgacccgct gcttgcaggc gccctttttc ctcgactttc agggcctcat tctctatcag
    24541 tgtctccctg ttcatgatgc acggaaagcg gggtctttca cgggccccgg gagagtgaaa
    24601 accatggccg aagaacttca gcacctgatc gagcgaatcc agaaagaggg cgtggagaag
    24661 gccgacgccc aggcggcgaa gatcatctcg caggccaagg aaaaagccgc cgcccacgtc
    24721 cgggaggccg aggagaaggc caagacgctg gtggccaagg gcgagaagga cgcccaggcc
    24781 ttcatggagc gcagcatccg cagcctcgag caggccgccc gcgacctcct gatcaccgtt
    24841 ggccagggcg tggagaatat cttgagcgac ctcgtggccg agtccgtgga caaggcgatg
    24901 acgatccagg tcctcgaaca gatgctcgtg aagatggccg aggcctacgc cgcccgcggc
    24961 ggcgaggaga gccggatcga gttgctgatc ggtccgcagg accagcagga catcgtcaag
    25021 ttcttcggcg accgctacaa ggagcagctg gtccgcggcg tcaagctgca cgtcgacaac
    25081 gacatcttca agggcttcaa ggtctccttc gcggacgggc gcgtgcagca cgacttcacc
    25141 cgcgaggcca tcgccgagtc gctcgttaat ttcctccggc cgcagttggc cgagatcgtc
    25201 caccgcgtgg cgcgggaagg ttcgcaaacg gacaagggat gacccggcgt gaagccctac
    25261 tacctggtgg ccagtctgcc gacgctggtc cttggcgacc cggcgcctct cgatgtcgcg
    25321 tccttcctgg cggcctgcgc caacctgttg agcgaggagg agttggcgga ggtgaccctg
    25381 atgctcgagg ggcgggtggg ggaatccggc gccgccttcg cgaaggactg gctccgggtc
    25441 gatacccagt tgcggaacgc cgtcgcgcgc gtccgcgccg gcaagcggtc ggccgaagcg
    25501 cgttccttcc tgcgcgagca cgagggctac gatgtcagcc tggagaaggc cgtcacggac
    25561 gcctacacgc ggcccaatcc gctggagcgg gagctcttcc tggatcggca ccgctggcag
    25621 cggctggatg aactcgtcct ggcggagccg ttcgggttcg ccgccgtgct ggcctatgcc
    25681 ttgaaactcc gcctggtcct gcgctgggcg gacctcaacg acgaggccgg cgggcagcgg
    25741 ctgacggagc tgctcaagga attggacaag agcgaaggca aaacctgaac accgaacact
    25801 ttgccgcccg ggtggcggaa tggcagacgc aagggactta aaatcccttt tccgaaaggg
    25861 agtgcgggtt cgagtcccgc cccgggcacc cggataacgt tgaagatact acagcaaaat
    25921 gaaccgcaga ggtcgcagag agtcgcagag gccggagggc tttacctctg ggagcctccg
    25981 tgccctctgt ggttagttct gcccgggaga gcgccatgag cgagaacatc ggaaagatcg
    26041 tcggggtgaa cggcaacctg ctgaccgtcg agttcaacca gccggtcacg cagaacgaag
    26101 tcggcttcgc cgcgctcggc gacctgaagc tcaagagcga ggtcatccgc atccgcggcc
    26161 ggcgcgcgga gctccaggtc ttcgaggata cgaccgggct gaaggtcggc gacccggtgg
    26221 agttcaccgg cgacctgctc gccgtcgagc tcgggccggg cctgctgacc cagatttacg
    26281 acgggctcca gaacccgctg ccggcgctgg ccgagaagtg cggttttttc ctccagcgcg
    26341 gcgcgtacct ccagtcgctc gaccggcagc ggaaatgggc gttcacgccg tcggcgaaga
    26401 agggcgacac ggtgcaggcc ggcgacaagc tcggcagcgt gccggagggc atcttcgagc
    26461 accggatcat ggttccgttc ggcgtgcgcg ggaagtgcac ggtggaagac gtggcggccc
    26521 agggcgagta ccgcgtcgag gacgtcgtgg cgaagatcag gaacgagcgc ggcgacgtgc
    26581 tcgaggtccg gatgatgcag cgctggccgg tgaagttccc gatccgggcc tacggcgagc
    26641 ggctccgccc cgtcgagccc ctgatcacca aggtccgcat cgtggacacg ttcttcccgg
    26701 tcgcgcgcgg cgggacctat tgcatccccg gccccttcgg cgccggcaag acggtgctcc
    26761 agcagatcac cagccgatac gcagacgtgg acgtcgtgat catcgccgcg tgcggcgagc
    26821 gcgcgggcga ggtggtggag accctgcgcg agtttccgga gctgaccgac ccgcgcacgg
    26881 gcaagagcct gatggaacgc acgctgatca tctgcaacac gagctccatg ccggtcgccg
    26941 cgcgcgaggc gtcggtctac accgccgtca ccatcgcgga gtactaccgg cagatgggcc
    27001 tgcacgtcct gctcctggcc gactcgacca gccgctgggc gcaggcgctg cgcgaggtct
    27061 ccggccggct cgaggagatc ccgggcgagg aggccttccc ggcctacctg gaatccgtga
    27121 tcgcggcctt ctacgagcgc ggcgggctgg tgaagctgcg cgacggcggc gtgggctcgg
    27181 tgacgatcgg cggcacggtc agcccggccg gcggcaactt cgaggagccg gtgacgcaga
    27241 gcacgctgaa ggtcgtcggc gcgttccacg gcctctcccg cgagcggtcc gatgcgcgcc
    27301 gctatcccgc catccatccc ctggagagct ggagcaagta cccgagcttc gtcagcgacg
    27361 aggatgtgcg cgaggcgcgc cacgtgctgc ggcagggcaa cgaggtcggg cagatgatga
    27421 aggtcgtcgg ggaagagggc acctcgttgg acgatttcgt cgtctacctc aagggcgagt
    27481 acgtcgacgc ggtctatctc cagcaggacg cctaccacgc cgtggacgcg gcgacgtcgt
    27541 ccgcgcgcca gcagtacgtg ttcgagcggc tggtgaagat cctgcggacg aagatggcct
    27601 tcgacgacaa ggacggcgcc cggaagttct tccagcagct gacccagacg acgaaggact
    27661 ggaaccgcgc gccgatgccc ggcgacgatg cgtcgccggc gagcggaagg cgtgcctgcc
    27721 accgccgtcc gtccagcgtg ccggtgacca cgacctcgcc gtcgagcgag tcgagcgccg
    27781 cgctgacgac gatcggctcc ccggcgtaga ggtcgggcac gctggccggg taggcctccg
    27841 cgcgacctgg ccaggcgatc gcgatgtcgg tcagcaccgg actttcgagc tgcaccagca
    27901 gcgcgcccat cttctgcgcc acctcgcgga cgtcgccgat catcgtgtgg ctgccgcgcc
    27961 cgaactgcgc cgccttgacc atgaagggcg agttcggcgc cgagcccaat ccgacggtga
    28021 acaggcgcgg gtcggagagc cgctcgcgga tcagcgtgaa cagcgccgcc tcgttgccg
//
LOCUS       DAOOTK010000029        27884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1209_length_27884_cov_6.346222, whole genome shotgun sequence.
ACCESSION   DAOOTK010000029 DAOOTK010000000
VERSION     DAOOTK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27884
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_27884_cov_6.346222"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK634_05255"
     CDS             <1..249
                     /locus_tag="PK634_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOW97176.1"
                     /translation="RAAESNALAMHATMIAAWPPVIYWKPESLAVMKRVAELRAQGLA
                     VYFTMDAGPNLKLLFERKDEFALGAKFSGLQVVAPFGP"
     gene            complement(264..1634)
                     /locus_tag="PK634_05260"
     CDS             complement(264..1634)
                     /locus_tag="PK634_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOW97177.1"
                     /translation="MRRAPQRIFTVSLILAGGFVPDASGATVQVTQRSIPAGIVDLSM
                     MVDTPTNFMTVSAPMEASSLRFTHWTVNGARSNDAFGRAVNPAWFEVHESVDAVAHYM
                     EAPVDSDADGVPDWFEVHFFDSLTNTASSDADADGWSLLEEYRRDGHPRISNSFYSGG
                     ISMRHSEPVCVNLAELFSWSVASDPGGLIPSRSGLATNGVMITATSHWREISGYCFGY
                     WTLNGARQADALGTALSRLTFTVTTSMTAVAHYVPPTQDTDGDGLPDWWESRFLGRFD
                     YGPNADPDSDGFGLLQEFRWELHPVVSNAFLPGGVSMRNSEQVPVVRADPLADVDGDG
                     LPNWWEEQYFGGPTNATPLADSDYDGLPNQDEMAAGTDPTSRLSCLQLEGSVLPDAVD
                     GFVIRWQSVTGRTYSIQRGTNLMWRSSISLIADGICGQPDFTSHTDTTAETEGPYFYS
                     IGIEAP"
     gene            complement(1644..3254)
                     /locus_tag="PK634_05265"
     CDS             complement(1644..3254)
                     /locus_tag="PK634_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOW97178.1"
                     /translation="MKRALRSVFCLTLSLAGPVYAESMPQDSWYFSHQWGGYGSAEGK
                     FNVPRGVAVGPDGRVYVADYTNKRIQVFEADGTFVRTWSCPSPWGLAVSSGGQVFVAG
                     SRTNCILVYEADGTFVRQWGSRGAGSGQFDLPTGVSVAPDGRIWVADSGNHRIQVFET
                     DGTFVRSLGRYGSLDGQFSSPRGAAVGPEGRVYVADYGNQRVQVLEQDGTFVRQWGPV
                     AEARSVTVLPGGRVFVPVSNQILVFESDGTLLRELNLVGLSEAAVAPDGRVLATEWDG
                     HTLHVFEPTYRTFAFNGSIPSPGVVRCRQRPGYSYLDIDYLVADPDSPTVTVAVAAFE
                     GGTNSLERFVRISTLLEGTEAQVGPNVAANTTNRISWNVGADWPADYVELKVRVMASD
                     GRGLLDIGFITLPTNGPEPALTISASPITHDDMLPLWIWLLATNDASIHLSSGLVYGV
                     GGAYDGALLASNTLTLPQGRAFLFDRMGLREATAGEVSRAKTGPGGVTNLWAPRITVG
                     PNRWPGGVNEYGFDTGKWGADAWWVVKE"
     gene            3467..3760
                     /locus_tag="PK634_05270"
     CDS             3467..3760
                     /locus_tag="PK634_05270"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97179.1"
                     /translation="MGLVVISGEEKAVGSRAGMKRTVETSPLYRAWPAKVAADLPEVR
                     AAESNALAMHATMIAAWPPVIYWKPESLAVMKRVAELRAQGWPYTSRWMPGRI"
     gene            3960..7826
                     /locus_tag="PK634_05275"
     CDS             3960..7826
                     /locus_tag="PK634_05275"
                     /inference="COORDINATES: protein motif:HMM:NF028070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOW97180.1"
                     /translation="MTTSRIDSAVALALMLVGTVCSAVPFHVTYLWHMHQPIYYPYKS
                     VNDTTEFNFSVAGVWDGDRYNAYRNWPKDAVQQGADRGMDHAGAQVSYSGSLAENNNN
                     LWGYNSWVGNYRWARNGLKTSLNNPRLDLVGIPYHHSLMPLTFKESMVMQIRLHKEQY
                     KDVWDTGGQYSKGFWPPECAFAEWIIPALVDEGMEWVIVDNGHLFRTVPDFPWNDGSS
                     CRPNKADVNNPGSTALNSEWVQLQNVWAPTQVLAPWSYQPHYVQHVNPWTGVRQKIIA
                     VPAGRYEGNENGRGGYGAFKPENVWGSHVAKNNNAAKPMMILCHSDGDNYGMKNSDAW
                     NGQHGNFLSMCQANADFDHTSVQDYLSMYPPDTNDVIHVEPGSWVGIDGGTPYFDKWL
                     SYENRDGEMPDMWSWSVLVAGINWVLTAEALESGYSMNDVEWGIGTDTAKAWHYYLNA
                     ETSCYWYWDYDRANPWDGNTTRAVNMAIGEANKVLGRHSGSDPVAPSIFPPQRQPYNP
                     GGKMWNESSNASSDFDVWTFVEDVSGLALVKLCWRVDLDGVNPVNETDNEVYAQNAAK
                     VTAWSNATMTASWWPSVKGPNVPDPANRAQRYLGTVSGQHDAMIDYFVEAVDTKGNTN
                     RSDIFHVWVGESTGGGTGPYVEFDPASPDGCNPVTIKYKKSGSPLGAGQVYAHVGRNG
                     WQDTITPDPAMTSSGDYWTYVYSVPTNTTEMNVAFNNGSGVWDNNGGQDWDLAVSNCG
                     TNGGGSGTNASTVAFSPTAPNGCVDVTITYTANDGTLKSAQPVYIHVGHNDWQDVIDP
                     DPTMTALGGGAWQYTYSAPVGTYEINCVFNNGAGTWDNNNKNDWKVTVTGCEDITGLV
                     ITTPASNVTVGADTTQVTVSGLAEAAITGWLHWSNSLTGGSGTCAAGASWSAAGIPLA
                     QGANVIAIAGTNQPVSGTTTTAADNASATAYNTGWTNNQNAGTGFAAWSLYTSTSDVN
                     QAGAFVCSNAPQCNIGTKAWGLYANNGQLSEAKRSFNAALTTADVFFAKFENGYIDTG
                     RGAGIALQNAAGETLWQFFFNGGDTNYSITGNSTDIGWTGAGLEISFRLTATNAYSVQ
                     ILPYGSSTRTVTGSLENLSDKTVARFRVWNYSTGGGSDYDVFFNDPRVTRPGAGGAGT
                     WYADTVTVTRLSGFVDSDGDGMDDAWEQANFGNLTAAHATSDWDQDGAPDGDEFLAGT
                     GPKDTGSVLRASQLVTDGHSGGYLVRWVSVTGRYYDVMFTTNLMDGFAPLVPATNLPG
                     IPPVNVWTDVVPGAQQRFYRIKTGRE"
     gene            complement(7857..9521)
                     /locus_tag="PK634_05280"
     CDS             complement(7857..9521)
                     /locus_tag="PK634_05280"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOW97181.1"
                     /translation="MYALHILTGPWKGKRVTVREPSLLIGRDAECPLRLPDDEISRKH
                     VTIEERPDGLHIRDLGSLNGFLVNGQPAREARLQEGSIIELGRTRLQVRLAAAPARPS
                     RRRIGWLQGWTAAAVAALLMGQLVFLLALSMRGVKLLKVVEKTVALSTPAGQPKATVP
                     SAAPPEVGLAPETEPAPPVVSNELSQLRADVEDLRRQVAEATAPAAPAAVEPVPPPPA
                     PAPLPEPAPAPEPTPVEAATPAETVPVSEPAPAPAEDPLVARAKAMLGEAAAEIQKVN
                     LLEADTLLERIQIMAPDFLPAYIERARLYEQRGLLNKAGEQWAIVLQKSIGNPLYEQA
                     AAERIRLARAAAFVPPPSVVPRRREPAPAEPRLPRRVRLASLEQEKLPVTEGYEEMRL
                     LRIAVRAMASERQLDPDDIRVAVTFFDEEYTSKEVVPTTVSVPSSPLPVSGEGSSGGP
                     YAVTATYMVPAGQRAKEEKASGRRFRYHGYAVRVYYREQLQDEGARPKALLEKLRALP
                     SPFHKPSSPAPKPAATNLPAIPAAPAPADPPAPKSEPLDVDAPVGR"
     gene            9839..10453
                     /locus_tag="PK634_05285"
     CDS             9839..10453
                     /locus_tag="PK634_05285"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOW97182.1"
                     /translation="MKLILASRNRHKLEEIRTLFDLPGLELSSALDHPEIPDVEEDGK
                     TFEANAIKKATALARATGGWALADDSGLEVDALGGAPGVYSARYAGEPVSYPANNAKL
                     LKALEGAADRRARFRCVVALSSPSGKTRTVEGRCEGRIIHGIRGAQGFGYDPLFVPDG
                     FDQTFAEMEAGAKNAISHRGRALQKARAAWHDFLMTDPAEWPAP"
     gene            complement(10650..10982)
                     /locus_tag="PK634_05290"
     CDS             complement(10650..10982)
                     /locus_tag="PK634_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW97183.1"
                     /translation="MQFQDSRMGQAWVIEVGGRVDAAGAALLEAHCQQGLGRGETRLV
                     VDLAGVDFMASAGLRALLSLAKKVKPKGKLVISGARGPVKEIFDLAGFTTILPICDTL
                     EQAAGLVM"
     gene            complement(11053..11472)
                     /locus_tag="PK634_05295"
     CDS             complement(11053..11472)
                     /locus_tag="PK634_05295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW97184.1"
                     /translation="MPDELRLTIANRLDELERAEAEISAFMDRHRVPPARRYAASLAL
                     EEMLSNIIKYGYDDAAEHAIGIRVESGARELALTLEDDGHPFDPTALAAPDTSQRIED
                     RSIGGLGIHLVRRMTDGMTWRREGGKNVVRIVIRRDG"
     gene            complement(11488..11826)
                     /locus_tag="PK634_05300"
     CDS             complement(11488..11826)
                     /locus_tag="PK634_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW97185.1"
                     /translation="MIITHTRQDALALVRIEGRLDAAAAPAAEKQLTDLFAAGARAVA
                     LDGSALEYISSAGLRVLLSLTKKIKAAGGRIALGGLQPQVKEIIEIAGFSSIMPVFAS
                     AEEARRVCAG"
     gene            complement(11823..12182)
                     /locus_tag="PK634_05305"
     CDS             complement(11823..12182)
                     /locus_tag="PK634_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW97186.1"
                     /translation="MQLQITGEEGPIIRLALSGALDLAGVREVELKFLAHTASRGGPA
                     LVDLSGVTFMASLGMRMLLEASRALQRKGAALGLLNPSALVEDALRAAGIADLVPVFH
                     DEARALAALLSNPEKTP"
     gene            complement(12191..12838)
                     /locus_tag="PK634_05310"
     CDS             complement(12191..12838)
                     /locus_tag="PK634_05310"
                     /inference="COORDINATES: protein motif:HMM:NF017530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HOW97187.1"
                     /translation="MLTFAEEIVLLALDEKEGVITELPHQALHAAMSGAVLMELCLLN
                     RVDVDAKQMKVVDTTPTGEPILDAVLKDLQGSHPEHTLAYWLNRMAGHHAHEILRLSF
                     TGLVRKGVLREEKHRFLWVFPDRRYPRLNDREFIEVRARLRQALLGTDIPEVRDIVLV
                     SLLNACRLIPRILADDELPRAHARIAQLSRLDLIGQALAQSIREIEFALSLPMML"
     gene            complement(12970..14643)
                     /locus_tag="PK634_05315"
     CDS             complement(12970..14643)
                     /locus_tag="PK634_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOW97188.1"
                     /translation="MALAWLAAGPALAEPPLLVVYPGGWHAAVTNFAAFKQRLGYDVR
                     LTNHWDATAGQTNSPATLRAALLSFYSSLSATQQPGCVLLVGSFQALPAPVFRVAASD
                     APYYSDIYFSIPDVNFDANGNGTNGEYGAAGDLTSAQFDGFFTALSNKLVVGRIPVAA
                     TAGIETVRRLLDASVAFERETGARKSRALLTAGRIDTSTPADSWEYVVRELAGRLGSN
                     YPARTFVTVAHVASNYTNRAGIDYAVEGDDVSTGYTEGQNIVRGLWQSNDACAFLCNV
                     SHGSSIYDFALRRNGAGLPASVHAAIVLSMSCASYDLGTAAFTAGVAAAYLGSTAVVT
                     PDVATLASAPRMVSADVQQRAAIDILGLTQTVGRVFREEFDYYVDQIPAAGWLTYLMD
                     PAGVLRNVIGFQIIGDPTLRREYPDGDADGLLDPEEATLGTLPGDADTDGDGLPDGYE
                     FLTPTLDPLADNGPDVDGDGSLNGDEWIAGTDPLSDSDYPCLAWTGDLALSWNSVTGR
                     LYTVQQATNAPPFEWQDCSAAATGTGARVEFEPDTAPARVLYRMSISRP"
     gene            complement(15412..16188)
                     /locus_tag="PK634_05320"
     CDS             complement(15412..16188)
                     /locus_tag="PK634_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOW97189.1"
                     /translation="MRTRIRPWLCCAVPALILAGALASPARAGDRPTVVSTVPAVGAV
                     EVDPAMTEITVTFDQDMAGGFSWTGGGPDYPPTTGRPFWRDARTCVLPVKLEAGHYYR
                     VGINSKSHQNFRNQSGLPAPTAAIYFTTAGASEELQAKTRKPTVVEMTPANGATDVDP
                     ATTELRVTFSVPMGGGFSWTGGGPAYPAGVEGKGPYWTVGQLTCALPVKLEPNHTYRL
                     NLNSFSHKNFQSAGGVPLEPVTYTFSTGAGGPTPPAPPLP"
     gene            16394..16582
                     /locus_tag="PK634_05325"
     CDS             16394..16582
                     /locus_tag="PK634_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97190.1"
                     /translation="MLLVSKWILIVAGLLLVLDAAAIVAGVPNPLFGWPMPCPITLAL
                     LGIGLVLFCLASAAFRRP"
     gene            16609..17256
                     /locus_tag="PK634_05330"
     CDS             16609..17256
                     /locus_tag="PK634_05330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97191.1"
                     /translation="MYTIAQQPGPVVGLKYRPSLAPGRDDNVDFYNEFYRPVPREFSA
                     ANQEVLRQALLSVPAPRCIVEIGVQRNPLPESSTGILLAHKPGDCLYIGVDIEDKSHL
                     DNASRRIHTLKMDSADRGRVYRQMEAGGAKTIDFLFIDGWHSVNQCLADWQYVERLAP
                     DGVVVMHDTNVHPGPVAVFEAIDEALFIKAKYCTEGPDWGISVVRRRAVAGEGRV"
     gene            complement(17303..17824)
                     /locus_tag="PK634_05335"
     CDS             complement(17303..17824)
                     /locus_tag="PK634_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97192.1"
                     /translation="MLWEYSLRGNHEHVVRSGSPEHRAQADGAIREYQRQRDEDLQRL
                     LGPDLARIFLQYAEGHDFSRFIRDLKTDLADQGRELPVPAERALLDLMWTAARETGKN
                     SDSGRTDEEQWRIEYAWQSNVLARAGAFLEPDTIRLLDQQFEQFYAIARMGEKSAGQK
                     ESERGHAGKEIAP"
     gene            complement(17936..19972)
                     /locus_tag="PK634_05340"
     CDS             complement(17936..19972)
                     /locus_tag="PK634_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOW97193.1"
                     /translation="MNPKDKRHDPDTIPMSGGAADVTQTMIQEGMLQAPARPGARGAV
                     GRFEILRPLAAGGMGQVFLAREPVTDGRVAIKMIRPRYRGEEWVVRRFLAEAQHMYRM
                     SHPHILKVMEVSDRKEGPYYVMPFIEGGSLAQAIKPGQPLPEDRVLEIARQVADALQY
                     AHARGIIHRDIKPANVLLDNEGRAYLTDFGLLRTVFNDTMLDPDKSAPEGTAAYMSPL
                     AASGKAEDTRCDIYSFGALLYEMLTGCKPYEGPNPKAVADRILAGPPRPILAVNRAAS
                     PALARIAEWCMARELRDRYAEMKDVVRDLDRAGQKEAPLGPHGAAEGQAVMVLKLSGK
                     RSVGKALAAAGTFLQGLCAVSLLWTIIHMIVAHLAADPQQPVSLAQRLGSAAAPAAFG
                     FLLGGVAGWFLLALALWACRYRAPWFYQAMHIIAIPWLLVFPAGTLASVLVLIHLREH
                     KAEFAAEPPAARPTLFMYFESAAVVAIAVFIAGLFGHVAYNMARRQARPSSAAQPASS
                     AAQDRCARAWRVWNEQKYEEAERLFLQATQADPRLADAWNGLGWSRQNMGQTQNAREA
                     FERCVALDPKNAGALNGLGWIAKSERKQHEAIRHWELAIAAEPGATAALAGLASTELE
                     LGYYDLAAKHAQSWLNLEPGNAEAKRILEQARQKRNIMDDLTPRQKLSADLGRD"
     gene            complement(19969..20496)
                     /locus_tag="PK634_05345"
     CDS             complement(19969..20496)
                     /locus_tag="PK634_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97194.1"
                     /translation="MNESSAQVLGVNAGKLAQLLDSGCPVADWRPEELGALFSHQMTA
                     PVQFDLAGFDPAFAERIRLLAGAQGLLVKSFRDLFLHPHPPAALLEAVKDFAKRAAAH
                     REAVLPKAIARMLYYLSISAALVRCGRRITSLSNKDLLTGLRWALGQEWVEKDLHGLL
                     EEGARQLESSRGEAT"
     gene            complement(20622..21269)
                     /locus_tag="PK634_05350"
     CDS             complement(20622..21269)
                     /locus_tag="PK634_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97195.1"
                     /translation="MNELLTAYSPVLRGYLRLVMRLRPEQAEDMVQDFVTRRILEGRL
                     LQRADRTRGRFRSFLLKCFLNFVRSELRKEKAAKRGPPPSQMLNLDDHAQDIADPCQE
                     RRAFDALWARQVLGAALERMQKDCEEKGRQDVWTVFEQRVLNPIFSDNPPRPYEELVT
                     ALGLKSPLQASNLLITAKRGFQRALEAVVRETVANETDIRAEIAALKRALGGVTG"
     gene            complement(21653..22573)
                     /locus_tag="PK634_05355"
     CDS             complement(21653..22573)
                     /locus_tag="PK634_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97196.1"
                     /translation="MRRIRYLVEYCFVRLLLFLLDRLPLSFCARLANGAADIAFRLHA
                     SRRRIAIDNILVSGITADRREAERIARASFRHFARLVAESLKSDAYFSRHPWTECVDW
                     RISPELQQILDDPSRGLILACGHLGNWEIAAQLLSHRKPVVGITRPMNNPYVNRLMQA
                     RKPRNRFELTPKHDANMARLVSALKEGKVLAIMIDQHARDRGMMIDFFGRPAATHTAI
                     ALLHLVTRTPLCFGACVETAPMQFRFHASGPLRFEPTGDKQQDIRRILEHLTRELETA
                     IRAHPEQYMWGHRRWRDVKPAPPSGTPALR"
     gene            complement(22570..22818)
                     /locus_tag="PK634_05360"
     CDS             complement(22570..22818)
                     /locus_tag="PK634_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOW97197.1"
                     /translation="MTREEILGKLRGIMGRTSQAKVDWSRVNEQSSIASLGIDSLAML
                     DLMYDLQQEFGIEFEPQDVVNVSTVGDLAAFIEGRMGA"
     gene            complement(22823..24064)
                     /locus_tag="PK634_05365"
     CDS             complement(22823..24064)
                     /locus_tag="PK634_05365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOW97198.1"
                     /translation="MEKRRVVVTGMGMVTPLGLDESSVCARLFGGQGGIRRITSFDPA
                     TFKSKNGAEIDPEALAAALTAFELKPSDRATDMALAASGHALKQAGLLPAGPGTPGED
                     TAVLIGSGSGPSHAVTESWKAYAAQGARGVRPTTVPRCMLNVLSSQVSIRFGLKGPNY
                     VLVAACSSSTMALGIGYRMILHGYARRVLCGGSDSMFTAPLYTGWDNLGVMSRSPDPD
                     KACRPFDAGRDGFVLGEGAGAVVLESLEEARSRGARIRAEIHGYGETSDATHITRPSV
                     EGQAAAMTAALRSAQMAPGDIDFVNAHGTATKANDVCEAEAIRGVFGSEADRVFVVSS
                     KSFFGHLLGAAGIVETLSAILCLEAGRLHANLNLDQPDPMCNVRLVGRESQAIPLRTC
                     MKNSFGFGGGNAVMILGQYKE"
     gene            complement(24078..24824)
                     /locus_tag="PK634_05370"
     CDS             complement(24078..24824)
                     /locus_tag="PK634_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW97199.1"
                     /translation="MDSLSGQAILITGSTRGIGRAIAAAAASRGATVGIHGRDAQHVQ
                     DICASLAASGGEALACPGDFADPENAREVVRAFLKAAGRLDGLVHCAGTGKARAFRAL
                     TLPDWRATFRVNLEAALLASQEAYLWMRQQKKGAIVHIASLAAHGPGRWMGADYAASK
                     AGLVSLTKSLALEAARFGIRVNAVSPGMVETDMTAALTETLKKNIGIPMNRFARPEEV
                     AEAVLFLLSDASSYLTGQVLHVDGGLWMES"
     gene            complement(25511..27052)
                     /locus_tag="PK634_05375"
     CDS             complement(25511..27052)
                     /locus_tag="PK634_05375"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HOW97200.1"
                     /translation="MKHFRRVLYVLGVALALTTSAKAPDPARSAELFDQAAAELDLRG
                     DMLVYFQIEGLVEGLLGKIKDVAGRIAEREDSAAAAVDIVGRIDSFLRTQGLYDGVSI
                     GISSRPRPDGYYDVKSFIARKPADPAPLFWRMMGGAPRELRVLDLIPADAAVVLSADF
                     RPADVWAFLQAAVQEVGGEQPYGELRAAVEELKTTAGADLDALIASLGGEVAFSLQLS
                     RDRTVELPLDSQRLAVPVPSFLLALGVKDDSLLQLLLAQIREGDLALETRQADGQTTY
                     VAPVVTEEPFPIQIALAQHENYLLVASHPDVMAESLKAFQSGKGLKSEPGFQAITKDL
                     PTLHNGLIFVDERFRTTVYEIQMTALREEMPDEEAELAVEVMNQLFGWSKEGSLASVR
                     VVKPNGILTHAVTPTGGKEVALAGAAMPIGLLAAVAVPSFVKARSRAQEAQMINVVRM
                     VDAAKQQWALENSKEDGADVTEDDIAPYLPAGILDLPPGHTLHINPLGEDPTITRPDG
                     STVTL"
     gene            27544..>27884
                     /locus_tag="PK634_05380"
     CDS             27544..>27884
                     /locus_tag="PK634_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOW97201.1"
                     /translation="MEWSQRQGADIDALLKPRALFAFARLWSAWANPETKAETVSCAI
                     VTCATNDLIRQAHERMPVLLRRDQEAVWLDPALKEPSDLLPLLQPYPADQMELYAVFS
                     GEVSLENNGGFS"
BASE COUNT         4678 a         9240 c         9376 g         4590 t
ORIGIN      
        1 cgcgcggcgg agtccaacgc cctggcgatg cacgcgacga tgatcgcggc atggcccccg
       61 gtgatctact ggaaacccga atccctggcc gtcatgaaac gcgtggcgga acttcgagcg
      121 caggggctgg ccgtatactt cacgatggat gccgggccga atctgaagct tttgttcgag
      181 cgaaaagacg agttcgctct gggagcaaag ttctccggtc tccaggtcgt cgcgccgttc
      241 ggcccgtgaa cggcggggcg ccgttacggc gcttcgatcc cgatggaata aaaatacgga
      301 ccctctgtct ccgcggtggt atcggtgtgg ctcgtgaaat ccggctgtcc gcaaatgccg
      361 tcggcgatca aagagatcga cgagcgccac atgagattgg tgccgcgctg gatcgagtac
      421 gttctgcccg tgacactctg ccatcggatc acgaaaccat ccacggcatc gggcagcaca
      481 gagccctcca gttgcaggca ggagagccgg ctagtcgggt ccgttccggc ggccatttca
      541 tcttgattcg ggagcccgtc atagtcgcta tccgccaggg gcgtggcgtt ggtcggcccc
      601 ccgaaatact gttcttccca ccagtttgga aggccatccc cgtccacgtc cgccagggga
      661 tccgcccgca ccacgggcac ctgttcggag ttgcgcatgg agacgccgcc gggaagaaag
      721 gcgttggaaa ccacaggatg aagttcccat cggaattcct ggagcaggcc gaacccgtcg
      781 ctatcggggt cggcgttcgg gccgtaatcg aagcggccca aaaagcggga ttcccaccag
      841 tccggcagcc catcgccatc cgtatcctgc gtggggggaa cgtagtgggc cacggcggtc
      901 atggaggtgg tcacggtgaa ggtcaaccgg gaaagcgccg tgcccagggc atcggcctgc
      961 cgcgcgccat tcagcgtcca gtagccaaaa cagtagcccg atatttcgcg ccagtggctc
     1021 gttgcagtga tcatgacgcc gttggtggcc agtcctgatc gcgacgggat gagcccccca
     1081 gggtcgctgg cgacggacca gctgaagagc tccgccagat tgacgcacac gggttccgaa
     1141 tggcgcatcg agatgccgcc ggagtaaaaa gagtttgaaa tgcggggatg cccatcccgg
     1201 cggtattcct ccagcaggct ccacccatcc gcgtctgcat ccgacgaggc ggtgttggtc
     1261 aggctgtcga aaaaatgcac ttcaaaccag tccggcacgc cgtcggcatc ggagtcaaca
     1321 ggggcctcca tgtaatgggc cacggcgtcc accgactcgt ggacttcaaa ccaggccggg
     1381 ttgaccgccc gtccgaaggc atcgttcgat cgcgcgccgt tgacggtcca gtgcgtgaag
     1441 cgaagggagg acgcctccat gggagcggac acagtcatga aattcgttgg ggtgtccacc
     1501 atcatactga gatccacgat acccgcaggg atcgagcgct gtgtcacctg caccgtggcg
     1561 cctgacgcgt cgggcacgaa gcctcctgcc agtatcaggc tgacggtaaa gattcgttgg
     1621 ggcgcgcgcc gcatgatcgc atcctattcc ttgaccaccc accaggcatc ggcgccccac
     1681 ttccccgtgt cgaaaccgta ctcgttgacc ccgcccggcc atcggttcgg gcccacggta
     1741 atccgcgggg cccagagatt ggtcacccct ccggggcctg ttttggcccg ggagacttca
     1801 ccggcggtcg cctctcggag gcccatgcga tcgaacagaa aggcgcgccc ctggggcagg
     1861 gtcagggtat tactggccag caacgcgcca tcgtaggcgc cgcccacacc gtataccagg
     1921 cccgaagaga ggtggatgga ggcgtcgttt gtggccagca gccagatcca taggggcaac
     1981 atatcgtcgt gcgtgatggg agaggcgctt atggtcaggg cgggctccgg cccgttggtg
     2041 gggagcgtga tgaacccgat atcaagcaag ccccgaccgt cgctggccat cacccggact
     2101 ttcaattcca cgtagtccgc cggccaatcg gcccccacgt tccaggaaat ccggttggtg
     2161 gtattggcgg ccacgttggg accgacctgg gcttcggttc cctccagtag cgtgctgatc
     2221 ctgacaaacc gctccagcga attggtcccc ccctcgaagg ccgccacggc cacggtcacg
     2281 gtcgggctgt cggggtcggc cacgagatag tcaatatcca ggtaggaata acccggcctc
     2341 tgccggcagc gaaccacgcc gggcgaggga atgctgccgt tgaatgcgaa ggtccggtag
     2401 gtcggctcaa acacgtgcag ggtgtggcca tcccactccg tcgccagcac ccggccgtcg
     2461 ggggcaacgg cggcttcgga caggccgacg agattcagtt cccgcaggag cgtgccgtct
     2521 gattcaaaaa ccaggatctg gttgcttacg ggaacaaaca cccgtccgcc gggaagcacg
     2581 gtcacgcttc gtgcctcggc cacaggaccc cattggcgca caaacgtgcc atcctgttca
     2641 agaacttgaa cgcgctggtt gccgtaatcc gctacataca cccgtccctc aggccccacc
     2701 gcggcgcccc ggggagatga aaactgtcca tcgagcgagc cgtagcggcc cagcgatcgc
     2761 acgaaggtgc cgtctgtttc aaatacctgg atgcgatggt tgccgctgtc cgcgacccag
     2821 atccggccgt ccggcgccac cgagacgccc gtgggcaaat caaactgccc gcttcccgct
     2881 ccccggcttc cccattggcg cacgaacgtg ccatcggctt catacaccag gatgcaatta
     2941 gtcctgctcc cggccacgaa cacttgtccg cctgatgaca cggccaagcc ccagggagag
     3001 ggacaactcc atgtgcgaac aaacgtgccg tccgcttcaa acacctgaat gcgcttgttg
     3061 gtgtaatcgg ccacatacac ccggccatcg ggtccaacgg caacgccgcg cggcacattg
     3121 aacttcccct ctgccgaacc gtatcctccc cactgatgac tgaaatacca ggagtcctgc
     3181 ggcatggatt cagcgtagac cggcccggca agcgacaggg ttaaacagaa gacagagcgt
     3241 aaagcccgtt tcatggcgcg cgcacctcct gactgtacgc ggcggaatcc ccagcggtca
     3301 gtgcttcagc cgctctagtt cagtttcgac ctgcccgctg cgttattcaa gcactaattg
     3361 cagcggcccg cgatcccgct gcgcgggctg atattcgtgc ttcggccaag gaagtcgttc
     3421 cgcttgctat cggcgtccgg gttcgcggcg ctggccggac ctgcggatgg ggctcgtggt
     3481 gatctcgggc gaggagaagg cggtcggctc gcgggcgggg atgaagcgca cggtcgagac
     3541 atcgccgctc taccgcgcct ggcccgcgaa ggtggccgcc gacctgccgg aagttcgcgc
     3601 ggcggagtcc aacgccctgg cgatgcacgc gacgatgatc gcggcatggc ccccggtgat
     3661 ctactggaaa cccgaatccc tagccgtcat gaaacgcgtg gcggaacttc gagcgcaggg
     3721 ctggccgtat acttcacgat ggatgccggg ccgaatctga aggttttgtt cgagcgagaa
     3781 gacgagcccg ccctcgcgtc ggaatttaat ggctttgagg tggttgcgcc gttttcgcgt
     3841 tgagcggcag tgcggaagct cgctaaacgc cgctcgttct tttgcctgct aaagcgcttt
     3901 acaggaatgc ggggcccggg tttgtttaga gcattcgctt tgcacaaagg atatccgcca
     3961 tgacaacttc gcgcattgat tctgctgtcg ccttggccct gatgctggtc ggcaccgtgt
     4021 gctccgccgt gccgttccac gtgacgtacc tgtggcacat gcaccagccg atctactatc
     4081 cgtacaagag cgtcaacgat actaccgaat tcaattttag cgtggccggc gtgtgggacg
     4141 gcgaccgcta caacgcctac cggaactggc cgaaggacgc ggtgcagcag ggcgcggacc
     4201 ggggcatgga ccacgcgggc gcgcaggtga gctattccgg ctcgctcgcg gagaacaaca
     4261 acaacctgtg gggctacaac tcgtgggtcg gcaattaccg ctgggcccgc aacggcctga
     4321 agacttcgct caacaacccg cgcctcgacc tcgtcggcat tccctaccac cacagcctga
     4381 tgccgctgac gttcaaggaa tcgatggtca tgcagatccg cctgcacaag gagcagtaca
     4441 aggacgtgtg ggacacggga gggcagtact cgaagggctt ctggccgccg gagtgcgcgt
     4501 tcgcggagtg gatcatcccg gccctggtgg acgagggcat ggagtgggtg atcgtggaca
     4561 acgggcacct gttccgcacc gtgccggact tcccgtggaa cgacggcagc tcgtgccgcc
     4621 cgaacaaggc cgacgtgaac aaccccggct ccacggcctt gaacagcgag tgggtccagc
     4681 tccagaacgt ctgggcgccg acccaggtcc tcgccccgtg gtcctaccag ccgcactacg
     4741 tgcagcacgt caacccctgg accggcgtac ggcagaagat catcgcggtc ccggcggggc
     4801 ggtacgaggg caacgagaac ggccgcggcg ggtatggcgc cttcaagccc gagaacgtct
     4861 ggggctccca tgtcgccaag aacaacaacg cggccaagcc gatgatgatc ctgtgccaca
     4921 gcgacggcga caactacggc atgaagaact ccgacgcctg gaacgggcag cacggcaact
     4981 tcctgtccat gtgccaggcc aacgccgact tcgaccatac ctccgtgcag gactaccttt
     5041 cgatgtaccc gccggacacg aacgacgtca ttcacgtcga acccgggagc tgggtcggca
     5101 tcgacggcgg cacgccgtac ttcgacaagt ggctctccta cgagaaccgg gacggcgaga
     5161 tgccggacat gtggtcgtgg tccgtcctcg tcgccgggat caactgggtg ctgacggcgg
     5221 aggcgctgga aagcggctac tccatgaacg acgtcgagtg gggcatcggc accgacaccg
     5281 ccaaggcctg gcactactac ctgaacgccg agacctcctg ctactggtac tgggactacg
     5341 accgcgccaa cccgtgggac ggcaacacga cgcgcgcggt gaacatggcg atcggcgagg
     5401 cgaacaaggt gctggggcgc cacagcggct ccgatcccgt cgcgccgagc atcttcccgc
     5461 cccagcgcca gccgtacaac ccgggcggca agatgtggaa cgagtcgtcg aacgcgtcct
     5521 ccgatttcga cgtgtggacg ttcgtcgagg acgtcagcgg tctcgcgctg gtcaagctgt
     5581 gctggcgcgt ggacctcgac ggcgtgaacc cggtgaacga gaccgacaac gaggtgtacg
     5641 cccagaacgc ggccaaggtc acggcgtgga gcaacgcgac gatgaccgcc tcctggtggc
     5701 cctcggtcaa ggggccgaac gtgcccgacc cggccaaccg cgcgcagcgc tacctcggga
     5761 ccgtttcggg ccagcacgat gcgatgatcg actacttcgt cgaggccgtg gacacgaagg
     5821 gcaacaccaa ccgcagcgac atcttccacg tctgggtcgg cgagagcacg ggcggcggca
     5881 cggggccgta cgtcgagttc gatcccgcct cgcccgacgg gtgcaacccg gtgacgatca
     5941 agtacaagaa gtccggcagc ccgctcggcg ccgggcaggt gtacgcgcac gtcggccgca
     6001 acggctggca ggacacgatc acccccgacc cggcgatgac cagctcgggc gactactgga
     6061 cctatgtcta ttccgtgccg accaacacga ccgagatgaa tgtggccttc aacaacggct
     6121 cgggcgtgtg ggacaacaac ggcgggcagg actgggacct ggccgtgtcc aactgcggca
     6181 ccaacggcgg cgggagcggc accaacgcct ccaccgtggc cttcagtccc accgcgccga
     6241 acggctgcgt ggacgtgacg atcacctaca cggcgaatga cggaaccctg aagagcgcgc
     6301 agccggtcta catccacgtc gggcacaacg actggcagga cgtgatcgac ccggacccga
     6361 ccatgaccgc cctgggcggc ggagcctggc agtacaccta ttccgcgccg gtcggcacct
     6421 acgagatcaa ctgcgtgttc aacaacggcg cgggcacctg ggacaataac aacaagaacg
     6481 actggaaggt gacggtgacc gggtgcgagg acatcacggg cctggtcatc accaccccgg
     6541 cctccaatgt cacggtcggc gcggacacga cccaggtcac cgtgtccggg ctcgcggagg
     6601 cggcgatcac gggctggctg cactggagca acagcctgac cgggggcagc gggacctgcg
     6661 ccgccggcgc atcctggtcc gcggccggca tcccgctggc gcagggcgcc aacgtcatcg
     6721 ccatcgccgg cacgaaccag ccggtatccg gtacaacaac gacggccgcg gacaacgcct
     6781 ccgcgacggc ctacaacacg ggctggacga acaaccagaa cgccgggacg gggttcgcgg
     6841 cttggtcgct ctacaccagc acctctgacg tcaaccaggc gggcgcgttc gtctgcagca
     6901 acgccccgca gtgcaacatc ggtaccaagg cctggggcct gtacgcgaac aacgggcaac
     6961 tctccgaggc caagcggtcg ttcaacgcgg ccctgaccac ggcggacgtg ttcttcgcga
     7021 agttcgagaa cggctacatc gacaccggac gtggcgcggg catcgccctg cagaacgccg
     7081 ccggcgagac gctgtggcag ttcttcttca acggcggcga cacgaattac agcatcaccg
     7141 gcaacagcac ggacatcggc tggaccggcg ccgggctgga aatcagcttc cggctcaccg
     7201 ccacgaacgc ctactccgtc cagatcctgc cgtacggcag ctcgacgcgc acggtcaccg
     7261 gtagcctcga gaacctgtcc gacaagaccg tcgcgcgctt ccgcgtctgg aactacagca
     7321 ccggcggcgg cagcgactac gacgtgttct tcaacgaccc gcgggtcacg cggcccggcg
     7381 cgggcggcgc ggggacttgg tacgcggaca cggtcaccgt cacccgcctc tccgggttcg
     7441 tcgacagcga cggcgacggc atggacgacg cctgggagca ggcgaacttc ggcaacctga
     7501 ccgcggcgca cgcgacgtca gactgggacc aggacggcgc gccggacggc gacgagttcc
     7561 tcgccggcac cggcccgaag gacaccggca gcgtgctgcg ggccagccaa ctcgtgacgg
     7621 acggccattc gggcggctac ctcgtgcgct gggtcagcgt gaccggccgg tactacgacg
     7681 tcatgttcac cacgaacctg atggacgggt tcgcgccgct ggtcccggcg accaacctgc
     7741 ccggcatccc gccggtgaac gtgtggacgg atgtggtgcc gggcgcgcag cagcggttct
     7801 accggatcaa gacgggccgg gagtaggccg ggcggccggc tcgcgcggcg ccgtttttac
     7861 cgcccgaccg gcgcgtcgac atcgagcggt tcggacttcg gagccggagg gtcggcgggc
     7921 gccggcgcgg cggggatcgc gggcaggttg gtggcggccg gcttgggcgc ggggctggag
     7981 ggcttgtgga agggagaggg cagggcccgc agcttctcca gcagcgcctt cgggcgcgcg
     8041 ccttcgtcct gcagctgttc gcggtagtag acgcgcacgg cgtacccgtg gtagcggaag
     8101 cgtcggcccg acgccttctc ctccttcgcc cgctgacccg cgggcaccat gtacgtggcc
     8161 gtcaccgcgt agggcccgcc ggacgagccc tcgccgctga ccggcagggg cgaggacgga
     8221 accgagaccg tcgtggggac gacttccttg gaagtgtatt cctcgtcgaa gaacgtcacg
     8281 gcgacgcgga tatcgtcggg gtcgagctgc cgctcgctgg ccatggcgcg gacggcgatc
     8341 cgcagcaggc gcatttcctc gtagccttcc gtgaccggga gcttttcctg ctccagggag
     8401 gccagccgga cgcggcgcgg cagcctcggc tccgccgggg cgggctcccg tcggcgcggc
     8461 acgacggacg gcggcgggac gaaggcggcg gcccgcgcca ggcggatgcg ctcggcggcg
     8521 gcctgctcgt aaagcgggtt gccgatgctc ttctgcagca cgatcgccca ttgctcgccg
     8581 gccttgttga gcaacccccg ctgctcgtac aggcgcgccc gctcgatgta cgcgggcagg
     8641 aagtcgggcg ccatgatctg gatgcgctcc aggagcgtgt cggcttccag caggttgacc
     8701 ttctggatct cggccgcggc ctcgcccagc atggccttgg cgcgcgcgac cagcgggtct
     8761 tccgccgggg ccggcgccgg ttcggagacg ggaacggttt ccgccggcgt cgccgcttcg
     8821 acaggcgtcg gctcgggcgc cggggcgggc tcggggagcg gcgccggggc gggcggcgga
     8881 ggaacgggct cgacggcggc cggggccgcg ggcgccgtcg cctccgctac ctggcggcgc
     8941 agatcttcca cgtccgcgcg cagctgggaa agctcgttgc tcacgaccgg cggcgcgggc
     9001 tccgtttccg gcgccagtcc aacctcgggc ggcgcggcgg acggaaccgt ggctttaggc
     9061 tggccggccg gagtcgacag cgccacggtc ttttccacca ccttcagcag ttttacgccc
     9121 cgcatggaaa gcgccaggag gaagaccagc tggcccatga gcagcgcggc gacggcggcc
     9181 gcggtccagc cctgtaacca cccgatccgt cggcgggagg gccgggccgg cgcggcggcc
     9241 aggcggacct gcaagcgcgt gcggcccagc tcgatgatcg atccctcctg cagccgggcc
     9301 tcccgcgcgg gctgtccgtt gacgaggaat ccgttcagcg agcccaggtc gcggatgtgc
     9361 aagccgtccg gccgctcctc gatggtcacg tgctttcgcg agatctcgtc gtcgggcagg
     9421 cgcagggggc attccgcgtc gcgcccgatg agcaggctcg gttcgcggac ggtcacccgc
     9481 ttgcccttcc aggggccggt caagatgtgc agcgcgtaca tgtcgtatcc gccgtggagc
     9541 gtgggcagtc tgtatcagga cgggcgggag cgtcaacccg ttagtggccg ccgctccgct
     9601 ccctctatca tctgtaacaa atatgctcac ggatgtgaag cgctatgtta tgacttgccc
     9661 ggcgctgggc ggccgcgcgg ccgggcctgg ccccgaacgc tgcccgcccg acaggaatcg
     9721 cgcggctcgc tctcgaacga acccggccgc gcgcgccgcc ggcgatgcgg cagagacggc
     9781 gtccgactga cgcgctttct ggttgcgcgc gccgggccgg ccgctgatga tgggccgcat
     9841 gaaactcatc ctcgcctcgc gcaaccgcca caagctggag gaaatccgca cgctgttcga
     9901 cctgcccggg ctggagctgt ccagcgcgct ggaccacccg gagatcccgg acgtcgagga
     9961 ggacgggaag accttcgagg cgaacgcgat caaaaaggcg acggccctcg cgcgcgcgac
    10021 gggcggctgg gcgctcgcgg acgattcggg cctggaggtg gacgccctgg gcggcgcgcc
    10081 cggcgtgtac tcggcccggt acgccggcga gccggtaagc tacccggcca acaacgcgaa
    10141 gctgctgaag gcgctcgagg gcgcggcgga ccgccgcgcg cggttccgct gcgtcgtcgc
    10201 gctgtccagc ccttccggga aaacccggac cgtcgaaggc cgctgcgagg gccggatcat
    10261 tcatggaatt cgcggcgccc agggtttcgg ctacgatccg ctctttgtcc ccgacggctt
    10321 cgatcagacc ttcgcggaga tggaagccgg cgcgaaaaac gcgatctccc accgcggccg
    10381 cgcgctgcag aaggcccggg ccgcctggca tgatttcctg atgaccgacc cggccgaatg
    10441 gccggccccc tagcccgctt gctgcatgag cgttgcgacg ttcatgcaat aagcgcttct
    10501 gagcacgcga gtggcaatgg cgccccgctc ggcggggcgc ctcaccgcat agggccacgt
    10561 tgccgcggcc tacgcggctc gcgtggcggc ccttcgggcg acagcccgcg caaaatgcgg
    10621 gttggcgccg ttccggacgt tgacccgcct tacatcacca ggccggcggc ctgctccagc
    10681 gtgtcgcata tcggcaggat ggtcgtgaag ccagcgaggt cgaagatctc cttcacgggg
    10741 ccgcgggcgc cggagatcac cagcttgccc ttgggcttga ccttcttggc caggctcaac
    10801 agcgcgcgca ggccggcgct ggccatgaaa tccacgccgg cgagatccac caccaggcgc
    10861 gtctccccgc ggccgagccc ctgctggcag tgcgcctcga gcaacgccgc gcccgccgcg
    10921 tccacgcggc cgcccacctc gatcacccac gcctgaccca tgcggctgtc ctgaaattgc
    10981 atgtgattct cccgtccggt gttttcgctt ctctttacca gaaacccgcc cgcgcggcaa
    11041 cccggccgcg ggtcaaccgt cccggcggat gacaatgcgc acgacgttct tgccgccctc
    11101 gcgccgccag gtcatcccgt ccgtcatccg gcgcaccagg tgaatgccca ggcctccgat
    11161 ggaccggtcc tcgatccgct gcgaagtgtc cggcgccgcg agcgcggtgg ggtcgaacgg
    11221 gtgcccgtca tcctcaaggg tcaacgccag ctcccgcgca ccggactcga cgcggatccc
    11281 gatcgcgtgc tccgccgcgt cgtcgtatcc gtacttgatg atattggaaa gcatttcctc
    11341 gagcgccagc gaggcggcgt accggcgggc cggcggcacg cggtgccggt ccatgaaggc
    11401 ggaaatctcc gcctcggctc gctcgagctc gtcgaggcgg ttggcgatgg tcaggcgcag
    11461 ttcgtcgggc atgggcgcgc tccggcgcta ccccgcgcat acccggcggg cttcctccgc
    11521 cgaggcgaac acgggcatga tgctggagaa ccccgcgatc tcgatgattt ccttcacctg
    11581 gggctgcagc ccgcccagcg cgatgcggcc gccggcggcc ttgatcttct tggtcagcga
    11641 gagcagaacg cgcagcccgg cgctgctgat gtattccagc gcggagccgt ccagggcgac
    11701 ggcgcgcgcg ccggcggcga acaggtcggt gagctgcttt tcggcggccg gcgccgcggc
    11761 ggcgtccagc cggccctcga tgcgtacgag cgcgagggcg tcctgccggg tgtgcgtgat
    11821 gatcatggcg tcttctccgg gttcgacagc agcgcggcga gggcgcgggc ttcgtcgtga
    11881 aaaacgggca ccaggtcggc gatccccgcg gcgcgcaggg cgtcctcgac cagggcggag
    11941 ggattcagca gcccgagggc cgcgcccttc cgctgcagcg cgcgagaggc ctcgagcagc
    12001 atgcgcatgc cgagggaggc catgaacgta acgcccgaga gatccacgag cgccggcccg
    12061 ccgcgggacg cggtgtgggc gaggaatttc agctcgactt cgcgcacgcc cgccaggtcc
    12121 agcgcccccg acagggccag gcggatgatc ggcccttcct cgcctgtgat ttggagctgc
    12181 atgcgtcgtc tcacagcatc atcggcaggc tcaacgcgaa ttcgatttcg cggatggatt
    12241 gggccaaggc ctggccgatc aggtccaggc gggacaattg cgcgatccgg gcgtgcgcgc
    12301 gcggcagttc gtcgtccgcc aggatgcgcg ggatcagccg gcaggcgttg aggaggctga
    12361 ccaggacgat gtcgcgcacc tccgggatat ccgtgccgag cagcgcctgg cgcagccggg
    12421 cgcggacctc gatgaactcg cggtcgttaa ggcgggggta ccggcggtcg ggaaagaccc
    12481 agagaaaccg gtgtttctcc tcgcgcagca cgcccttgcg caccagcccg gtgaaggaga
    12541 gccgcaggat ctcgtgggcg tggtggccgg ccatacggtt cagccagtag gccaaggtgt
    12601 gctccggatg ggaaccctgg agatccttca ggacggcgtc caggatcggc tcgccggtcg
    12661 gcgtcgtgtc caccaccttc atctgcttcg cgtccacgtc cacgcggttg agcaggcaga
    12721 gttccatcag cacggcgccc gacatggccg cgtggagggc ctggtgaggg agttccgtga
    12781 tcacgccctc cttctcgtcg agcgcgagca gcacgatctc ttccgcgaaa gtcaacatag
    12841 ctaccagcga atcctagccc cgcgccggcg cgcgggcaac aggatattcg tcgtggagaa
    12901 cgcgaaaggc ccgcagggtt cacggcccct ccccgaaggc atcttcgcat cctcggcgat
    12961 tcactccgat cagggccggg aaatggacat ccggtagaga acccgcgccg gcgcggtgtc
    13021 gggctcgaat tcaacccgcg cgcccgtgcc cgtagcggcg gcggagcagt cctgccactc
    13081 gaacggcggc gcgttggtcg cctgttgcac ggtgtagagc cggcccgtga cgctgttcca
    13141 ggagagcgcg agatcgcccg tccacgcgag gcagggatag tccgagtccg acagcgggtc
    13201 ggtgccggcg atccactcgt ccccgttgag gctgccgtct ccgtccacgt ccgggccgtt
    13261 gtcggccagc gggtcgagcg tcggggtgag gaactcgtac ccgtccggca gcccgtcgcc
    13321 gtccgtgtcc gcgtcgcccg gcagggtgcc gagggtcgcc tcctcggggt ccagcaggcc
    13381 gtcggcatcg ccgtccggat actcgcgccg cagggtcggg tcgccgatga tctggaaccc
    13441 gatgacgttg cgcaggacgc cggccgggtc catcaggtag gtcagccagc ccgcggccgg
    13501 gatctggtcc acgtagtagt cgaattcctc gcggaacacg cgcccgacgg tctgggtcag
    13561 gcccaggata tcgatcgcgg cccgctgctg gacatccgcg gagaccatgc gcggcgcgga
    13621 ggccagcgtc gcaacgtccg gtgtgacgac cgccgtcgaa cccaggtagg ccgcggcgac
    13681 gcccgccgtg aacgcggcgg tcccgagatc gtaggaagcg caggacatcg agaggacgat
    13741 ggccgcgtgg acgctggccg ggaggcccgc gccgttgcgg cgcagggcga aatcgtagat
    13801 cgagctgccg tgcgagacgt tgcagaggaa agcacacgcg tcgttggact gccagagccc
    13861 gcgaacgatg ttctggccct cggtgtaccc cgtgctgaca tcgtcgcctt ccaccgcgta
    13921 gtcgatgccg gcgcggttgg tgtagttgga cgcgacgtgc gccaccgtga cgaacgtccg
    13981 cgccgggtag ttcgatccga gccgcccggc cagttcgcgg acgacgtatt cccagctatc
    14041 cgcgggtgtg ctggtgtcga tgcgccccgc ggtcagcagc gcccggcttt tgcgggcgcc
    14101 ggtttcccgt tcgaacgcga ccgaggcgtc gagcagacgg cgtacggttt cgatgcccgc
    14161 ggtggcggcg acggggatcc ggccgacaac gagcttgttg ctcaaggcgg tgaagaaacc
    14221 gtcgaactgg gcggaggtga ggtcccccgc cgcgccgtat tcgccgttgg tgccattccc
    14281 gttggcatcg aagttgacgt cagggatgga aaaatagatg tccgagtaat agggggcgtc
    14341 gctggcggcg acccgaaaga cgggcgcggg cagggcctga aaactgccga ccagcaggac
    14401 gcagcccggc tgctgggtgg cggacaggga tgaatagaag gagagcaggg cggcccgcaa
    14461 cgtggccggg ctgttggtct ggccggcagt cgcgtcccag tggttggtca ggcgaacgtc
    14521 gtaacccagc cgctgcttga acgcggcgaa gttggtcacc gccgcgtgcc agccgccggg
    14581 gtagaccacc aacagcggcg gttcggcgag agccggcccg gccgccagcc acgccaaggc
    14641 catgatccgc caactcttgt gcgagcaaag cacgcggggc gcctccttcc ctgactatac
    14701 cgggaagcgg cgaaaaaatt caggcgctgt ctggaaaagc gtggaatgca gtcgggcttc
    14761 gacctttctt ctgatggagc gggagccatc ttggatcgga ggtgcggaag agaaagcgaa
    14821 tacatgactg gcagaacata gaaatcaacc ggccaagaag aagggcggct tcgtcaggag
    14881 aacggtttcg ccggctggtc ccccctggca aacggcgcga caccgctcgc tgtcgcggtg
    14941 cgccgggccg gcgggctcca tcctgtcgcc ttgggccgca cgtcgcatcc ggatggatgc
    15001 aaaagcgaag ggcctatccc gtgccgcccg gggatgcaaa tcgcggcggg atgagtccgt
    15061 ggcctcgcca cgttcggcgc acggggcagg gtctcggggg ccctgcgcgg ccgtcgcatg
    15121 gatcgccatc tggctgactt gttttgcggg gcggttccgg gcgcagtctg aattcaaagc
    15181 gcgagacatg agggggacgg agtggcaggg cgcgctgaag ttggcgggcc ctgatgtgga
    15241 gggggcgctc atccgctgcg tcgttggcct cgcagcaacg cggctttcgg catcggacgg
    15301 agcgtgcgac aggcctatgc ctgcataccc gttgcgtcgc cggtcttgcc cgcgcggcct
    15361 gcggccttgt gagacgcctg cgctgctcgc gaggatggtg ccccgcccgt tctatggaag
    15421 aggcggcgcg ggcggggtag ggcctcctgc tccggtggaa aaggtgtagg tcacgggctc
    15481 gagcggcacg ccgccggcgc tctggaagtt cttgtgcgaa aagctgttca ggttcagccg
    15541 gtaggtgtgg ttcggctcca gtttgaccgg tagcgcgcag gtgagttggc cgacggtcca
    15601 gtaggggccc ttgccctcga cgccggccgg gtacgccggc ccgccgccgg tccacgaaaa
    15661 cccaccaccc atgggcacgc tgaaagtcac acgcaattcc gtagtggccg gatctacatc
    15721 cgtcgcgccg ttggccgggg tcatctcgac gaccgtgggt ttccgggtct tggcttggag
    15781 ttcctcgctc gcgcctgctg tggtgaaata tatggccgcc gtcggcgcgg gcaggcccga
    15841 ctggttgcgg aagttctggt ggctcttgga attgatgccg acgcggtagt agtgtccggc
    15901 ttccagtttc accggcaaca cgcacgtccg cgcatcgcgc cagaacggcc ggccggttgt
    15961 cggcgggtaa tccgggccgc cgcccgtcca cgagaagccg ccggccatgt cctggtcgaa
    16021 cgtgaccgtg atctccgtca tggccgggtc cacctctacg gcgccgaccg ccggcacggt
    16081 cgagacgacg gtcgggcgat ctccggcccg ggccggggag gccagcgcgc cggccagaat
    16141 gagcgcaggg acggcacagc acaaccaggg gcgaatgcgg gttctcataa tgactcctgc
    16201 ttgttgtctt tcaagtctca tgcggctgaa ggtgtagaca gtgggcgccg agacacgttc
    16261 acctttttgg gtagatcgcc attcgcgcgc ggcgccccgg cgccgttttc ccgcacgaat
    16321 cggagccggc cgattcgaag tggatattca gccgcccctc gtgcaatatc ccggggcaag
    16381 aaaaggaaac cccatgctcc tcgtgtctaa atggatactg atcgtggccg ggctgctgct
    16441 ggtgctggat gccgcggcca tcgtcgcggg agtgccgaac ccgctgttcg gctggcccat
    16501 gccctgtccc attacgctgg ctcttctcgg catcggcctc gttctgtttt gcctggcgag
    16561 cgcggccttc aggaggcctt gacaccgcgc gcactggaaa ggaaccccat gtacacgatt
    16621 gcccagcaac cgggtccggt ggtcgggctc aagtatcggc cgagcctggc gcctggtcgc
    16681 gacgataacg tcgatttcta caacgaattt taccgccccg tgccgcgcga gttttcggcc
    16741 gccaaccagg aggtgctccg gcaggccctg ttgagcgtgc cggccccgcg atgcatcgtc
    16801 gagatcggcg tgcagcggaa cccgctgccg gaatcgtcca ccggcatcct cctcgcccac
    16861 aagcccggcg attgcctgta tatcggcgtg gacatcgagg acaaaagcca cctggacaac
    16921 gccagccggc gcatccacac gctgaaaatg gattccgcgg accgcggccg ggtctaccgc
    16981 cagatggagg cgggcggggc gaagactatc gatttcctgt tcatcgacgg ctggcattcc
    17041 gtcaaccagt gcctcgccga ctggcagtac gtggagcgcc tcgccccgga cggcgtggtg
    17101 gtgatgcacg ataccaacgt gcaccccggc cccgtcgcgg tgttcgaggc catcgacgag
    17161 gcgctcttca tcaaggcgaa atactgcacg gagggcccgg actgggggat ctcggtcgtc
    17221 cggcgccgcg ccgtcgccgg agaaggccgc gtgtagaaaa gcgggccgga cagagggggg
    17281 cgcagggagt cgggccggca tcttacgggg cgatttcctt cccggcgtgt ccccgctccg
    17341 actctttttg cccggctgat ttctccccca tccgggcgat cgcgtaaaac tgttcgaact
    17401 gctggtcgag caggcggatg gtgtccggct ccaggaaggc gccggcgcgg gccagcacgt
    17461 tgctctgcca ggcatattca atccgccact gctcctcgtc cgttcgtccg gagtcgctgt
    17521 tcttcccggt ctcgcgggcg gcggtccaca tgagatccag caaggcgcgc tcggccggca
    17581 cgggaagctc ccggccctgg tcggccaggt ccgttttcaa atcacggatg aaccggctga
    17641 aatcgtggcc ctccgcgtat tgcagaaaga tgcgcgcgag gtccgggccc aggagccgct
    17701 ggaggtcctc gtcccgttgc ctctggtatt cccgaatcgc tccgtcggcc tgggctcgat
    17761 gttccgggga acccgagcgc acgacgtgtt cgtggttgcc gcgaaggctg tactcccaca
    17821 gcacctcctc cagggcgcgc tgggtctcgg gcgcgatgtg ttcgagtgcg tagaagttcc
    17881 gataccgaag ggcggcctgc tgcttgtgcg cgcgccgctg aaagaccagc agatactaat
    17941 cgcgacccaa gtccgcgctt aatttctggc ggggcgtcag gtcgtccatg atgttccgct
    18001 tctgccgggc ctgctccagg atgcgcttcg cctcggcgtt cccgggctcg agattcagcc
    18061 agctctgcgc gtgcttggcg gcaaggtcgt agtagcccag ttcaagctcg gtggaagcca
    18121 gcccggccag cgcggcggtc gcgcccggct cggcggcaat ggccagttcc cagtgccgga
    18181 tggcttcgtg ctgcttccgt tcgctcttcg cgatccagcc gagcccgttc agggcgccgg
    18241 cgttcttcgg gtcgagcgcg acgcagcgct cgaaagcctc cctggcgttc tgcgtttggc
    18301 ccatattctg ccgggaccag cccagcccgt tccaggcgtc ggccagcctg ggatcggcct
    18361 gcgtcgcctg caggaacagc ctctccgcct cctcgtactt ctgctcgttc cacacccgcc
    18421 aggcccgggc gcagcgatcc tgggcggcgc tggaagcggg ttgcgccgcg gaggaggggc
    18481 gggcctgccg gcgggccatg ttgtaggcca cgtggccgaa caacccggca atgaaaacgg
    18541 cgatcgccac taccgcggcg ctttcgaagt acatgaagag tgtcggcctg gccgccggcg
    18601 gttccgcggc gaactcggcc ttgtgttccc gcaggtggat caggaccagc acgctcgcga
    18661 gcgtccccgc gggaaagacc agcagccagg ggatcgcgat gatgtgcatc gcctggtaaa
    18721 accacggagc ccggtagcgg caggcccaca gcgcaagggc cagcaggaac cagccggcca
    18781 cgccgcccag gaggaatccg aaggccgccg gcgccgcggc gctccccagt ctctgggcca
    18841 gggatacagg ctgctgcgga tccgccgcca ggtgggcgac gatcatgtgg atgatggtcc
    18901 agagcagcga caccgcgcat aggccctgca ggaacgtgcc ggccgccgcc agcgccttcc
    18961 cgacggaccg cttcccgctc aacttcagca ccatgaccgc ctggccctcc gccgctccgt
    19021 gcgggccgag gggcgcctct ttttgcccgg cccggtcgag gtctcggaca acatccttca
    19081 tctcggcgta ccggtcgcgc agttcgcggg ccatgcacca ctccgcgatc cgcgcgagcg
    19141 ccggcgaggc ggcccggttg acggccagga tcgggcgggg cggcccggcg agaatccggt
    19201 ccgcgaccgc tttcggattc gggccctcgt acggcttgca ccccgtgagc atctcgtaga
    19261 gcagcgcgcc gaacgagtag atgtcgcacc gcgtatcctc ggccttgccc gaggcggcca
    19321 ggggcgacat gtaggcggcc gtgccctcgg gcgcgctctt gtcgggatcc agcatcgtat
    19381 cgttgaacac cgtccgcagc aggccgaagt ccgtcaggta ggcgcgcccc tcgttgtcca
    19441 gcagcacgtt ggcgggcttg atgtcgcggt ggatgatgcc gcgggcgtgc gcgtactgca
    19501 gcgcgtcggc cacctgccgc gcgatttcga gaacgcggtc ctccggcagc ggctggccgg
    19561 gcttgatcgc ctgcgccagg ctgccgcctt cgatgaaggg catgacgtaa tacggcccct
    19621 ccttgcggtc cgacacctcc atgaccttca ggatgtgggg gtgcgacatc cggtacatgt
    19681 gctgcgcctc ggccaggaac cggcggacca cccactcctc gcctcggtac cggggacgga
    19741 tcatcttgat ggccacgcgg ccgtccgtga cgggttcgcg cgcgaggaaa acctggccca
    19801 tgccgcccgc ggcgaggggc cgcaggattt caaaccggcc cactgcgccc cgcgcgcccg
    19861 gccgggccgg cgcctggagc atgccttcct ggatcatggt ctgcgtgaca tccgccgctc
    19921 ccccggacat ggggatcgta tcgggatcgt gccgtttgtc tttggggttc atgtcgcctc
    19981 cccccgcgag gactccagtt gccgcgcgcc ttcctccagc aacccatgaa ggtctttttc
    20041 gacccactcc tggcccagcg cccagcggag gccggtcagc aggtccttgt tgctcaacga
    20101 agtgatccgc cgtccgcacc gtaccagggc cgcggagatg gagaggtaat acagcatgcg
    20161 ggcgatggcc ttgggcagga cggcctcgcg gtgcgccgcg gcgcgcttgg cgaaatcctt
    20221 gaccgcctcg agcagggcgg cgggggggtg ggggtgcagg aacaggtccc gaaaactttt
    20281 cacgaggagg ccttgggcgc ccgccagcag ccggatccgc tcggcgaacg cgggatcaaa
    20341 tcccgccagg tcgaactgca ctggcgccgt catctggtgg ctgaacaggg cgcccagttc
    20401 ctcgggacgc cagtctgcca cggggcagcc gctatccagc aactgtgcga gtttcccggc
    20461 gttgaccccg agaacctgcg cggaagattc attcatggtt cacctcggta atggccctca
    20521 ccttgtacta cgcggcgcgg ggcgagatcc tgcgccggcc gggcggttgt tgtgctttgt
    20581 cgaagagggg ccgattatct tcccttgcgc gggagccggt gctaaccggt tacgccgccc
    20641 agcgcccgtt tcagcgcggc gatctcggcc cgaatgtccg tttcgttcgc caccgtttca
    20701 cggactacgg cttccagggc gcgctggaag ccgcgcttgg ccgtgatcaa caggttggag
    20761 gcctggaggg gggatttcag gcccagggcg gtcaccagtt cctcgtaggg gcggggcggg
    20821 ttgtcggaga aaatgggatt caggacgcgc tgctcgaaaa cggtccagac atcctggcgc
    20881 cctttctcct cgcagtcctt ttgcatgcgc tccagcgcgg ccccgagcac ttgccgggcc
    20941 cacagggcgt caaaggccct tcgctcctgg cacggatcgg caatgtcttg ggcatggtca
    21001 tcgaggttga gcatctgcga tgggggtggc ccgcgcttgg ctgccttttc cttgcgcaat
    21061 tcgctccgga caaaattcaa gaagcacttc agtaaaaaag agcggaaccg gccacgcgtg
    21121 cggtccgcgc gctggaggag gcgtccttcc aggatgcgcc gggttacgaa atcctgaacc
    21181 atatcctccg cctgttccgg gcgcaaccgc atgactagtc tcaagtaacc gcgcagaacc
    21241 gggctataag ccgtcagcag ttcgttcatg gcgtccaggc cggcttcggg ttcaggggcc
    21301 tgggcatggg caacgagagt ccaacgggtt ggggggaaac gaaaagagtc ggcctcctgg
    21361 ttgcattccg gcatatacaa ggcacccctt gctggcgtcg cccatagtat cctcccccgc
    21421 gcgggcgaca caagagtggc gcctaaaaaa gcgcggaatg ccgcccccgt ccttcctttc
    21481 tatttgcagg ggcgccgagc ggtcgccctg gaaaggagcg ggagcgagag gctgcatcga
    21541 ggagtcggaa aatacagcag atcccgttcg gcctggaatt ggccggcggg gcagggttgg
    21601 ggaggggcga ggaacggctg tcgtccgagc gccaggtccg cccggaaccg gattagcgga
    21661 gcgcgggagt tccggaaggg ggagcgggct tgacgtcgcg ccagcgccgg tgtccccaca
    21721 tgtactgctc cggatgggcg cgaatggcgg tctcgagttc ccgggtcagg tgctcgagaa
    21781 tgcggcggat atcctgctgc ttgtcgcccg taggctcaaa gcgcaagggg ccggaggcgt
    21841 ggaaccggaa ctgcatcggc gccgtttcca cgcaggcgcc gaagcagagc ggcgttcggg
    21901 tcacgaggtg cagcagggcg atggccgtgt gcgtggccgc cgggcggccg aagaagtcga
    21961 tcatcatgcc ccggtcccgg gcgtgctggt cgatcatgat ggccagcacc ttgccttcct
    22021 tgagggcgga aaccaagcgg gccatgttcg cgtcgtgctt cggcgtcagt tcgaagcggt
    22081 tccggggctt ccgcgcctgc atgaggcgat tgacatacgg gttgttcatc ggccgcgtaa
    22141 tgccgacgac cggcttgcgg tgggacagca gttgcgcggc gatctcccag ttgcccaggt
    22201 ggccgcaggc caggatcagg ccgcgcgagg gatcgtccag gatctgctgc aattccggcg
    22261 aaatccgcca gtcgacgcat tccgtccacg gatgccggct gaaataggcg tccgacttga
    22321 gggattccgc aacgagccgc gcgaagtgcc ggaacgaggc ccgggcaatc cgttcggctt
    22381 cgcgccggtc ggcggtgatc ccggacacaa ggatgttatc gatggcaatg cgccggcgcg
    22441 acgcatgcag ccggaacgcg atatcggcgg cgccgttggc cagccgcgcg cagaaggaca
    22501 ggggcagccg atccaggagg aacaacaaca accgcacgaa acagtattcg accagatatc
    22561 gaatccgcct cacgcaccca tccgcccctc gatgaaagcg gccagatcgc ccacggtgga
    22621 cacgttcacc acatcctggg gttcgaactc gatgccgaac tcctgctgca ggtcgtacat
    22681 caggtcgagc atggccaggg aatcgatgcc cagcgaggcg atggaactct gttcgttcac
    22741 ccggctccag tccaccttgg cctggctggt ccgccccatg atgccccgca gcttcccgag
    22801 gatttcctct cgcgtcatgc gattactcct tgtactggcc caggatcatc acggcattcc
    22861 cgccaccgaa cccgaagctg tttttcatgc aggtgcgcag cggaatcgcc tgggactccc
    22921 tgcccacgag ccgaacattg cacatcgggt cgggttgatc aaggttcagg ttggcgtgca
    22981 gccgccccgc ctcgaggcag aggatggccg acagggtttc cacgatcccg gccgcgccca
    23041 gcaggtggcc gaaaaatgac ttcgaggaga ccacgaagac ccggtccgct tccgagccga
    23101 acacgccgcg tatcgcctcg gcctcgcaga cgtcattggc cttcgtagcg gtcccgtgcg
    23161 cattgacgaa atcgatgtcg ccaggcgcca tctgcgcgga gcgcagcgcg gccgtcatgg
    23221 cggccgcctg cccctccacg ctgggccggg tgatatgcgt ggcgtcggag gtttcgccgt
    23281 agccgtgtat ctccgcgcgg attcgggcgc cgcgcgaccg ggcctcctcc agcgattcca
    23341 ggaccacggc gccggcgccc tccccgagaa cgaacccgtc gcgcccggcg tcgaaaggcc
    23401 ggcaggcctt gtccggatcg ggcgacctcg acatgacgcc gaggttgtcc catccggtgt
    23461 acagcggcgc cgtgaacatg gaatcgctgc cgccgcagag cacgcggcgc gcgtagccgt
    23521 gcaggatcat gcggtagccg atgcccaggg ccatcgtgga cgaggagcag gcggccacaa
    23581 gcacgtagtt gggacccttg aggccgaacc ggatcgagac ctgggagctc aagacgttga
    23641 gcatgcaccg gggcacggtc gtgggccgca cgccgcgcgc gccctgcgcg gcataggcct
    23701 tccaggattc ggtgacggcg tgcgagggac ccgaacccga cccgatcagc acggccgtgt
    23761 cttcccccgg cgtgcccggc cccgccggga gcaggccggc ctgttttaag gcgtgccccg
    23821 aggccgccaa cgccatgtcc gtggcgcgat cggaaggctt caactcgaac gcggtcagcg
    23881 cggcggccag cgcctccgga tcgatttccg ccccgttctt cgacttgaac gtcgcgggat
    23941 cgaaagaggt tatccgccgg atgccgcctt gccctccgaa gagccgcgcg cagaccgacg
    24001 actcgtcaag ccccaggggc gtgaccattc ccattcccgt gacgacgacg cggcgctttt
    24061 ccatcgtgat cttcctttca ggactccatc cacaacccgc catccacatg caggacctgc
    24121 cccgtgaggt acgacgaggc gtcggacagc aggaacagga cggcctcggc gacttcctcg
    24181 ggccgggcga agcggttcat cggaatccca atgttttttt tcagcgtttc agtcagcgcc
    24241 gcggtcatgt cggtctccac catgcccggc gatacggcgt tgacgcggat gccgaagcgg
    24301 gccgcctcca gggccaggct cttggtcagg ctgaccaggc ccgccttgct tgcggcatag
    24361 tcggcgccca tccagcgtcc ggggccgtgg gcggccaggg aggcgatatg gacgatcgcg
    24421 ccttttttct gttgccgcat ccagaggtag gcctcctgcg aggccagcag ggccgcttcc
    24481 aggttgaccc gaaacgtggc ccgccaatcc gggagcgtca gggcgcggaa ggcccgggcc
    24541 tttccggtgc cggcgcagtg aaccaggcca tccagcctgc cggcggcctt caggaaagcc
    24601 cgcaccacct cgcgagcgtt ctcggggtcc gcaaagtccc ccgggcaagc cagggcctct
    24661 ccgccggaag ccgccaggct ggcgcagata tcctgcacgt gttgcgcatc gcgaccgtga
    24721 atgccgacgg tcgcgcctcg actcgccgcc gccgcggcaa tggcgcgtcc gatgccccgg
    24781 gttgaacccg tgatgagaat cgcctgtccg ctcaagctgt ccatttcgcc tgctcgtttg
    24841 ctccgcccgg aagataaaga tatccctggc caagtgcaag cggagcgtcc gggcgcggaa
    24901 tcgcgcgggg tatcgaacgg caagaccggg atgtttttcg tggaatgagc gccccctcgt
    24961 ttctttcttc tgatgagggg ccgcatgcgc cccgaaagga ggatctcgag aggctgccag
    25021 acgttggtct tcaatcgaag aaagggccgg gcgattccgc atcccgaggc gccccctccg
    25081 cttcgggaga acaactcccc ttggccgttg ctctcccgca ttgtgcatgg gagttcatgc
    25141 aagacgagga taggacctca tgcctgccgg ggcgctagcc cggataatgc gcgactggcg
    25201 cgcattatcc gcccaaaggg ccgccacgtg agccgcgtcg gccgcggcaa cgtggccgca
    25261 cgcggggatg tgcccgcgtt gcgggcacaa acggaaccca cgtacgtttt gtcgaatccc
    25321 tgattcgaca aaacgtcatg tcttctggca cgcgaattgc taatgcaaat cacgtgctca
    25381 gaggcgctta ttgcgtgagc gtcgctgcgc tcgtgaaata agcgggctat ccggccgggt
    25441 gaaagtgcgt ccttcccgat gcgccaatcc cggggcgttt cccgccgcct ccgggagagc
    25501 cggacgatta ttacagggtt acggttgacc cgtccgggcg ggtgatcgtt gggtcttcgc
    25561 cgagcggatt gatgtgcagg gtgtgtcccg gaggcaggtc gaggatgccg gcgggcaggt
    25621 acggggcgat gtcgtcttcc gtgacatcgg ctccgtcctc cttgctgttc tccagcgccc
    25681 actgttgctt ggcggcgtcc accatgcgca cgacgttgat catctgcgct tcctgggcgc
    25741 gcgaacgggc cttgacgaaa ctggggacgg ccacggccgc caggaggccg atgggcattg
    25801 ccgcgccggc cagcgctacc tccttgccgc cggtaggcgt cacggcatgg gtcagaatgc
    25861 cgttgggctt gaccacccgg acgctggcga gcgagccctc cttgctccac ccgaacaact
    25921 gattcatgac ttccacggcc aactccgcct cctcgtcggg catttcctcg cggagggccg
    25981 tcatctgtat ctcgtagacc gtggttctaa aacgctcgtc cacgaagatc agcccgttat
    26041 gcagcgtcgg caagtccttc gtgatcgcct ggaatccggg ttcggacttc aggcccttcc
    26101 cgctctggaa agccttcagg gactcggcca tgacatccgg atgggaagcg acaagcaggt
    26161 agttctcgtg ctgggccagg gcgatctgga tggggaaagg ctcctccgtc acgaccggcg
    26221 ccacgtaggt ggtctggccg tcggcctgcc gtgtttccag cgccaggtcg ccttccctga
    26281 tttgcgccag cagcaactgc agaagggagt cgtccttgac gcccagcgcc agcagaaacg
    26341 agggcacggg caccgccagg cgttggctgt ccagcggcaa ttccaccgtc cggtcccgcg
    26401 agagttgcag cgaaaacgca acctcaccgc cgagcgaggc gatcagggcg tccaggtcgg
    26461 ccccggccgt ggtcttgagt tcctcgacgg cggcccggag ttccccatag ggctgctcgc
    26521 cgccgacttc ctggacggcg gcctggagaa aggcccagac gtccgccggc cggaagtccg
    26581 ccgagagaac gaccgcggcg tcggccggaa tcaggtccag gacgcgcagt tcccgcggcg
    26641 ctccccccat catgcgccag aagagcgggg cgggatcggc aggcttccgg gcgatgaagc
    26701 tcttcacgtc gtagtaaccg tccggtcgcg gcctggaact gatgccgatg ctcacgccgt
    26761 catacaggcc ctgggtccgc aggaacgaat cgatgcggcc aacgatatcg accgcggcgg
    26821 ccgcggagtc ctctcgctcg gcgatgcgcc ccgcgacgtc cttgatcttg cccagcaagc
    26881 cttccaccag gccttcgatc tggaaataca ccagcatgtc gccgcgcaga tccagttcgg
    26941 cggcggcctg gtcgaataac tcggccgaac gcgccgggtc gggggccttg gccgaagtgg
    27001 tcagcgccag cgcgacaccg agaacataca ggactcgtcg gaagtgcttc atggatggtc
    27061 tccttggttg tccgcctctc tgccggccta ttgtccagca agacgtattc cggcgcaagt
    27121 ccgccgtgca atggctgggg agaacaccgt atgtccggcg tggagagttg gcgctcgggg
    27181 ggggggcggc ttgaaccccc tagcccgatc aatgctggcg cggttaaaat acacgtggac
    27241 tcacggccag cctcactacc ttcggaggtc tacgagatag gggcggtctg gcgcaagctc
    27301 ccttcgcatc tgcgaacagc gattctggtg atggtgcgcg gcgcgggtgc agctacccga
    27361 cgatagccgc cttgcattcc tccgtccgtg ctccaagttt cagccatgtg cgggcgccgt
    27421 accgtcatgt cgaagttccc cccccgagat gcacgactcc tggtcgctgc ccgaggatga
    27481 gcccgatctg aataaactcg tcccgtcgta caacatcgcg cctacccaga aggtgctggc
    27541 cgtttggaat ggtcccaacg gcaaggcgct gacatcgatg cgctattgaa accccgggct
    27601 ctattcgcct tcgccaggct ctggtcggcc tgggccaacc ccgaaaccaa ggcggaaacc
    27661 gtatcctgcg ccattgtcac ctgtgcgaca aatgacctga tcaggcaggc gcacgagcgt
    27721 atgccggtcc tcctgcggcg cgaccaggag gctgtatggc ttgatccggc cctgaaggag
    27781 ccaagcgatc tgcttcccct gctgcagcca tatcccgccg accagatgga gctctacgcc
    27841 gtcttctcgg gcgaagtctc gctggagaac aacggcgggt tttc
//
LOCUS       DAOOTK010000030        27609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1227_length_27609_cov_5.085868, whole genome shotgun sequence.
ACCESSION   DAOOTK010000030 DAOOTK010000000
VERSION     DAOOTK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27609
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1227_length_27609_cov_5.085868"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PK634_05385"
     CDS             complement(<1..890)
                     /locus_tag="PK634_05385"
                     /inference="COORDINATES: protein motif:HMM:NF021347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HOW97202.1"
                     /translation="MRDKEEFYGLLMEIEGKEYREYAKLIGDFDFTRYVLKINQIQDA
                     AAGQSTLVLTRVPQVIAGFPAHLMNTPVRRTALEDFLVRKLSARLESLARYDKEGISR
                     RRIPLYAPGQAILPRTSLVVTDEYIEARLYVDLPSRHGCVAADSAREIFFEDLPGVVN
                     ASLIFCNLNQREVEDFVNLMEDADQVRQVLPTRGWVGFVGEGALLARAGDTDMPDYEQ
                     MTPLAIDDPLAVEVDVPNAGRVRGFGIPAGITVILGDRYSGRVDLVRALAAGIYNHVP
                     GDGREWVVSLPDTVQIVAEAG"
     gene            complement(925..1608)
                     /locus_tag="PK634_05390"
     CDS             complement(925..1608)
                     /locus_tag="PK634_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOW97203.1"
                     /translation="MKIACSRCGKENEPTRLFCSNCGAKLNLDGKAWREPVRWGRFLT
                     RIVQVLILAGVGLLLWPVKPQGAVGGRAEAVAYYGKLRLLSEAVERRVMVVQTFSEAE
                     VNAYLAEVLKEHPELSSSEGLSKLGIGEINLRFRPEGVTVTVVALWGPLRMSYEIAGL
                     PRAGGGPFAFDVRGARWGHLPLPGVAAHWMVRRVELMFSGMERERTVLDGLQRVDVGG
                     GLVRVATDS"
     gene            complement(1614..1904)
                     /locus_tag="PK634_05395"
     CDS             complement(1614..1904)
                     /locus_tag="PK634_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOW97204.1"
                     /translation="MPTYEYECRRCGHRFEKFQAMKDRPLQRCPRCRGKIERLPGGGA
                     GILFRGAGFYATDYRSAGYRKAARADKSGESTKAAPAAGSAAKGDTTKSKAG"
     gene            2079..2963
                     /locus_tag="PK634_05400"
     CDS             2079..2963
                     /locus_tag="PK634_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOW97205.1"
                     /translation="MFDVEAYMGAHRSRIEKELDRYLPPAATRPAVLHEAMRYSVFAG
                     GKRLRPILCVATCEAVGGSAEPAWLPAAALELMHTYTLIHDDLPAMDNDVLRRGKPTC
                     HVVFGEANAILAGDALLTLAFEWMARSLPPPPYQPGHYVLELAEAAGSLGVIGGQVED
                     LAAEGQPPTAELVEHIHQRKTAALIRASIRIGAAAGRATEEEFNALTLYGGDIGLAYQ
                     ITDDILDATATTESLGKTAGKDQARRKCTWVEVFGLEASWQKAHALADRAIEALKPLH
                     RKGEALKSLAHFLVNRES"
     gene            complement(3154..4008)
                     /gene="purU"
                     /locus_tag="PK634_05405"
     CDS             complement(3154..4008)
                     /gene="purU"
                     /locus_tag="PK634_05405"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOW97206.1"
                     /translation="MKPNTAILLIHCPDQRGIVAALAGFIASENGNILHLDQHVDTEQ
                     NVFFARVEWALDGFRIPRERLGEAVEQLAGPRRMNWQLFFSDRRPRLAIFVSKQSHCL
                     YDLLARHEAGELDADIPMIVSNHPDLRPAAERFGIPFRVVSVTPGERAAVEEQATRLL
                     ESAGVDTVVLARYMQILSGDFVRRYPDQIINIHHSFLPAFPGAKPYHSAHARGVKIIG
                     ATAHYVTETLDEGPIIEQDVVRVSHRQTVGDLVRQGQDLEKVVLARAVRWHIEKRILV
                     YHNKTVVF"
     gene            4060..5511
                     /gene="gnd"
                     /locus_tag="PK634_05410"
     CDS             4060..5511
                     /gene="gnd"
                     /locus_tag="PK634_05410"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOW97207.1"
                     /translation="MSTADIGLVGLAVMGENLILNMESRGFTVACYNRTASKVEAFVQ
                     GRARGKKIIGCATLEDFCRALRRPRKVMLMIKAGAAVDAQIEALLPHLEAGDVIIDGG
                     NSLFSDSRRRAAFLAARGLLFVGTGVSGGEEGALTGPSIMPGGHEQAWPLLRPIFQAI
                     AARVDNAPCCEWIGADGAGHFVKMVHNGIEYGDMQMIAEAYALLKGALDLPADELSAV
                     FREWNADELNSYLVEITADILAKKDDETGRPLLDLILDSAGQKGTGKWTSQTALDLGA
                     AAPTMAEAVFARCLSALKDERMEASAHLAGPGRKSVGHRIAFIEVVRRALYASKICSY
                     AQGFQIMRAAAAEYGWTLNYGAIAVIWRGGCIIRAQFLGRIKDAFEKQPDLSNLLVAP
                     YFQKALKRCQGGWRRAVTAAVKLGIPVPAFSSALAYYDAYRSERLPANLLQAQRDYFG
                     AHTYERIDKPRGQFFHTNWTGRGGTTASTTYEV"
     gene            5420..6619
                     /locus_tag="PK634_05415"
     CDS             5420..6619
                     /locus_tag="PK634_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97208.1"
                     /translation="MNASTNRADSSSIPTGRGAAARPPRRPTRCEAPRLALVLAALAL
                     AADGRTWTSRSGSQIEADLESVQGEFAVLRAADGRQFKIQVAQLSDADQAFVRESAAS
                     PVPAPAGEPAATPAPPPAAPPAESAGTADPATAWIAFTAPDPMTSDKFPGLVATNLPR
                     MMLVGLQYGPAKTEVLYCAFETTDPQQPPSALHLYSPAAEGYTRTVTLQGTRRKVEES
                     RVTGYKDVRLKAAFGDVQVSATIEFICGIGRSDTVLLNAAVQLARGGAASSCLLGGYL
                     NNDFVYGAGTIPILPLLGKPSLEVKTWMMHGPVLTGFGQVGKLSVIPKTGMASTLAVE
                     VTERESGRSLDRLKGEFNEKKFSAGQPDRTINVSLQLEQGKKVAVKSVMDLGPALGKA
                     EKTSHFY"
     gene            6694..8127
                     /locus_tag="PK634_05420"
     CDS             6694..8127
                     /locus_tag="PK634_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOW97209.1"
                     /translation="MASRIKAAMLAIVGLTGPGPAMAQDIVPAPEPDVVYTIPIRGMI
                     EPALLYVIRRGVREAEAADARAIVFVMDTPGGTVDAAGEIVRTITGLDVPSYAFVENQ
                     AFSAGAIIALATKHIYMAPGSIIGDAMPILSTPFGGVQEMSEDLKEKSVAAVAAIIRT
                     AAEAGGHDKELAEKMVRRELEYKVGDEVISPAGQLLTLTNVEAEREVGDPPRPLLSSG
                     TVADLEAMLREVGLGNAAVRSLEITSAERVARFIAALAPLFLIGGLLGIYIEIKTPGF
                     GLPGILGIISLAIFFWGHHIAGLAGMEELLIFALGALLLAAELFVLPGFGAVGLIGIA
                     LILWALLGAMIQRYPGGPWMPAWQQVQFPLLKLSLALIGTAAIGALIARLLPSSPVFR
                     RLVLSQATRRSEGFAASEDTRSLVGRTGRALTPLRPAGTAEFGEQRIDVVTSGEYIGA
                     GSDVRVVESHGNRIVVEAVGGGPARGV"
     gene            8130..8627
                     /locus_tag="PK634_05425"
     CDS             8130..8627
                     /locus_tag="PK634_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOW97210.1"
                     /translation="MDQTILIYIILLLAGLLLIGIEIFVPGGVIGALGALALIVAMFL
                     GFKAFEPPGGIISALAIVLLTGLGTALWIRFFPRTSAGRHLTLSQDGRDFKSAPPELK
                     EWVGREGIAHSTLRPGGIALIDGHRVDVIAEGRWIESGRRIRVLNVEGTRVIVRDIAD
                     QEKTS"
     gene            8624..9835
                     /locus_tag="PK634_05430"
     CDS             8624..9835
                     /locus_tag="PK634_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97211.1"
                     /translation="MNLRLPSTLALLLLAASAGAAPLSFEAPEEPFVLDEEQALTNEL
                     WLVAPAADIRGAVQDDLFAVGGTLLVAGAMGRDVWALGNEVDLTGRAAGHARLLGRAL
                     DIRGSVAGNLIGLGGSVGLAADSEVGGDAVLVGEDVTVEGRIGGRAQIYAQRATLSGR
                     FGGTVRLYANDIVVHPGTEIRGDLEYTSSRDLILDERVALTGQLIRKEAAAPALRSTP
                     ASWSEALSIQLLLFSAALLVALPFLAVFPGYAGRAVRLLRQSGWKCALAGVAAFALFP
                     MAALILPLTIVGIPLAVVLLLLFILLLYASKFILALAVGGWLLRRRGALSFGRAISTL
                     AVGLALLYGLVNVPGMGLAIGLIILWLGLGSLVLAALAPPSDGAAEPPPLPEPAGGPP
                     PVPPFPGSKQP"
     gene            9852..10877
                     /gene="floA"
                     /locus_tag="PK634_05435"
     CDS             9852..10877
                     /gene="floA"
                     /locus_tag="PK634_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199596.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOW97212.1"
                     /translation="MLTQVVTVLFVLLAIAVLVLIGLFFYFLKVWIRAAMSGARVSLF
                     NLVGMKLRRVPPTLIVDARIRMVKAGMDLQTDPLEAHYLAGGNVINVVQALIAADKAN
                     IDLSFQRAAAIDLAGRDVYDAVRTSVNPKVIDCPDPAKGTTMLDAVAKDGIRLLVKAR
                     VTVRANLERLVGGATEDTIIARVGQGIVSAIGSSLSYKDVLENPDHISRKVLDSGLDS
                     QTAFEIVSIDIADISVAGTGARDVANVGAMLETERAEADKKLRQAEAEGRRAMAIAAE
                     QEMRARVQEMQAKVVEAQAEIPQAIGHAMRAGKLGVMDLMRMQNIMADTSMRESLAEG
                     GHETPKS"
     gene            10907..11512
                     /locus_tag="PK634_05440"
     CDS             10907..11512
                     /locus_tag="PK634_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97213.1"
                     /translation="MKHLLADSAYESLFWLVMAIVWVVVQVIARATKQQLRKGAAPRP
                     TPAAAKSESSLEEFLRSLGAEPLLPPKPAQELEPVPEAAPEPEPAPAIRIRKRRAPRP
                     AEPIAPAAETPPPPAALPRLAPEACVMAEGRRMRGMMVIPKIPVVRVQGLRVPSLSFT
                     GTTVNNIRPGPDRFPLKGREALRLAMLHRLILEPPHNIHPL"
     gene            11678..13363
                     /locus_tag="PK634_05445"
     CDS             11678..13363
                     /locus_tag="PK634_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HOW97214.1"
                     /translation="MHSNRWRRTIGYLCAVLTAGGISKATAQELIAWDSAADPVYDGS
                     WCDRQEGGGGWTTPWMLWTRDTGSCGYFLQTSTSNGDGDRTGDGDIDTNGRAWGLFAR
                     DGVTAWALRGFAPLTPWDTLCLSMDNGWIDGDGVVGMGLQTSDYQNRVEFYFVAGMAH
                     YILHDGDGPRDTGIPFTDEGLRLLFSIVDGQRYILTVIERETGIARTFSGRLSGEANA
                     AIELVRFFNYNANAFGADGAPRDLYVNSLALYTMRPDADERAPQIELLGSSMVTIECG
                     TAFADPGARAFDDMDGDLSDRIATSSDLNVRRPGAYTVTYRVADAAGNEGVATRTVRV
                     VDTIAPTLSRVSDQTVVPSTSAVLAPVFFRAPTATDNSDSVSVVCVPAPGSFLAPGAH
                     TVTCTATDSSGNRSQFVFRVTVLPFLRVAFQAPIQDDNKADDIEKDADQANLFRVGVP
                     MPHIVKLYDCKGRDVTAAVAPLVKLQLNVTEREQRGAGSVQVADPPEKASVVGGAKGA
                     MAPVGDTFQFNLDTQTFEAGTQNNARFFRSLVRAAYVAVPGVFFEEDAMLESR"
     gene            13460..13542
                     /locus_tag="PK634_05450"
     tRNA            13460..13542
                     /locus_tag="PK634_05450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13493..13495,aa:Leu,seq:caa)
     gene            complement(13672..14859)
                     /locus_tag="PK634_05455"
     CDS             complement(13672..14859)
                     /locus_tag="PK634_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97215.1"
                     /translation="MRGNRLASMSMYTELGKRFMRYRVPFLREKLLPMKLTVASTFLC
                     NHRCKMCDIWQVYRKAKAKLDEELTRADFQNIFEQLRTSLLYLDWTGGEPFLRQDMPD
                     ILGDAARICPRLSSVVITTNGLLTDRIVESVEKVARAMPRQIVAIGVSLDGTRDNHNF
                     IRGRDDAYETAVSTLAGLRKLSETRPNVEAKISYTISYRNAGKLETFDRDVLRPLGLN
                     MGDVGFGLEHTGGLYQTAFSPDKAVGRVSEEEFRALARNDIEYALARLKEDDLRLLQR
                     LKSMYRVFFLREIPGFLDQPTKLVMHCSATRNSLYMDPYGFLYPCIVWSRKLGHFKDG
                     IAALLRGELVRDTRRLIDRGQCPICWNACEAIPSLLTSYRLVGCVARGFADARAIRGL
                     LKR"
     gene            14914..16635
                     /locus_tag="PK634_05460"
     CDS             14914..16635
                     /locus_tag="PK634_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW97216.1"
                     /translation="MSAPADRVERWRQDALSVRTGTGAHPKDFDWLAKSIPCQAACPA
                     RTDVPAYLDAIAQGRFDEAYRINLADNVFPAVLGRVCTRPCEPACRHGWEELGSPVAI
                     CFSKRSAADFQDRREPVTLDPVFGPSGKSVAVVGGGPAGLSAARELARWGHRVTVYER
                     ESEPGGLLVQGIPRFRLPREAVAREIEQVRLCGVRILCSQPVGADPSLDTLLSSFDAV
                     IVAAGAQRLHTLPLPGSELGGLRHGLAFLREMNAGGRSKPGDRVVVIGGGFTAVDCAR
                     AARRLGASSVRVLYRRSEEEMHITPGEIREMKDEGISFETQAAPVSFSGSGGRIESAR
                     FIRTRIQDGPGGGRRDPLPVPGSEFDIACDAVLLAIGQAPDTAWLAGRTEPRLFTAGD
                     FASGSDSLIHAIADGKAAARRLDLFLMGAARLRDVAEIVHARRTGRTRAMDEIPRQEM
                     PVLPLPARLPEAEVETGFDRAAAQREAQRCYLCHYKFEIDNDYCIYCDRCLKAKPLPD
                     CIVKVSRLVHDEQDRIAGFIPSTGPEDYRMLYIDSSKCIRCGACRDVCPMACISLQRA
                     SWRAANA"
     gene            16632..17939
                     /locus_tag="PK634_05465"
     CDS             16632..17939
                     /locus_tag="PK634_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97217.1"
                     /translation="MTDATPSGPGPKRLVEAGIALLVVAAALLLATPAWRPPKLFPDS
                     LTYIQWDPIRPPAYCAFVALFGQGAALVQVQSLLSLLSWAFAGWMLGRGAGLFLLTLL
                     AMSLPVAQWNLMLLSESFSYSLVMLAIGLTLRLLRKWSWGGFAAWVAVAALLSFARLS
                     NLFITPFLALPFLARARTAWRAMGAASLALFLAGLWMSATRGADFERMELSNVIMGRI
                     LPDPEARAFFHARGMPLDERVMSYSGRLRSRAYRELWARAPEFRAWLTPEGMRTYRAW
                     LLRDGAAFRAAWRALAEGDSGIGNCLGYGGDVAPRPVAARLLPFYLWLGKLPPALWLA
                     LMLLLPLLDRWRDGPLSPRSLLLPALATAAYAQAFICFHGDADEVPRHMLIVGLLYRV
                     TILAGLCGAVRLAAGLFAGRKREAAPLPPSGQASAQEFAVQDR"
     gene            complement(17907..20066)
                     /locus_tag="PK634_05470"
     CDS             complement(17907..20066)
                     /locus_tag="PK634_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97218.1"
                     /translation="MHRNSRPRRENRTEAACRHVGVLLLLLMAGCLLAPPSAGAKTLK
                     VKIDGTGDYLTIRDAVEAAKDGDTVEIHPGFYYGPENLNSKISGKKLFIKGIVSGDLI
                     AIPEQFTRAFPQLADSRRLHQWKKPVICGQLAKDPAGEALIFGIFGTYRAFWIENAEV
                     ELEDLIIYRTSLLAADPSADNEGGAILVEKGSEVEIRDFEIRETIAATPAGVGGGIAL
                     LSSSRLLMENSVVNACGSTLKGGGVALLGGSQATLVQSAIDGGAGKGGGVFVSDGSLY
                     LSQCQVHGQAEAKGQSPDQCAGGGLLMENSGGVIINSTFSGCGARGYGAGVALKHSSP
                     FVTDCVFYRNEAWGGGGALAMWDDSRPALHKAIVVENTCGKYDRIGHMPLTAPIPNLN
                     GGGVLALASFPKISTSFFGQNGATGQGGGVMLTDSAAVLQDCIFMANGALMGGGLASI
                     GRLAPTNELPGIPPLDIYRCAWVWNGAADPSGLAEGCGGGMLVVDGIPHVDNSFFAQN
                     FAVWGGGAAIKELNIPLPERFRGQRYKYPEKSELRNCTFRENVAAQGGGAVHYTMNIG
                     WPYQNYYYLHHGIANSIFWGNDDAYSNQIYTAGATVYVDSSNVQNGPAGVHAWTGGKA
                     VWGAGNMAADPLFGNGVVLSSASPCRNAGDSSLVWPGETDIQGKARISGPAVDIGAYE
                     LPPSLSEYARWLLEHPSVFEEMQPSLPSSVLYGEFLR"
     gene            20326..20841
                     /locus_tag="PK634_05475"
     CDS             20326..20841
                     /locus_tag="PK634_05475"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOW97219.1"
                     /translation="MTQIACPIEEALARKYPEPVVLVTTRDHDGRANVMAAAWVCFAS
                     SEPWMFLLGIDAGARTYEVIVRTRQFVVAFPHEGMGRETLYAGTNHGHATDKFKETGL
                     AMQDASVVRAPLVRDAVANFECELVEIYQPGDCPLIAGKVVAAHANRDADLRRLCLVG
                     PDYRLGGLRPA"
     gene            20925..21401
                     /locus_tag="PK634_05480"
     CDS             20925..21401
                     /locus_tag="PK634_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HOW97220.1"
                     /translation="MVIGWDIKFRTAWPYYMAQSLAATAVVFLVLLVLHRTNAVVVAS
                     IGSSVYILFALPESPTAGPRRVIGGHAAGLLCGGLWALVPHTSPEGCAAVYALAIGSS
                     LFLMVVTDTEHPPASGTALGVAITGASLALGLSLMVSVTLLSLAHRLLRGRLKNLL"
     gene            21463..23283
                     /locus_tag="PK634_05485"
     CDS             21463..23283
                     /locus_tag="PK634_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97221.1"
                     /translation="MRILLITPPLLQFNTPYPAAPALAAFLKSRGLHVAQADLSLELI
                     LRLFSRRGVLDVARVMRRGNRPSTRHFLAHAREYAATVGAAAAFLQGRRPGLARRIAS
                     RRFLPEGPRFAQADPFELDRFPLPERARHLASLYVDDLADAIRDGVDPRFGLARYAER
                     LAVRAPSFAPIRKALEGPPTRVDRLIDQIARELIRRRRPDLVGITVPFPGAVYGAFRI
                     ARAARRMRPRPRVVLGGGYVSTELRDLSDPRVFDYADYMVLDDGEAPLLALAEHLEGR
                     RPSGRLIRTLARAGGRVVRHDDSEPDVPHRDKPAPDYGGLKLDRYLSMAETLNPMHRL
                     WSDGRWNKLMLAHGCYWRRCAFCDTTLDYIHRFDPAPAFRLANRIEQVRRQTGSRDFH
                     FTDEAAPPALLRALAEELVRRRIRIRWWCNIRFEKNFTPGLARLLARSGCIAVSGGLE
                     AATDRLLRLMNKGITVAQARRVMKAFSEAGIMVHAYLMYGFPTQTAREILEALDTVRR
                     LFKAGCLHSAYWHRFALTAHSPVAQDPAAYGIRLVPARRGGFARNELSYTEPKGFDHD
                     RFGPGLRKAVYNFMHGIGFDAAVRSWFDRGEYRGRSAPRP"
     gene            complement(23233..24483)
                     /locus_tag="PK634_05490"
     CDS             complement(23233..24483)
                     /locus_tag="PK634_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOW97222.1"
                     /translation="MSGRNDLRGALGLGLVVAALCGSAFPESHGRAAARPRVIVQSSG
                     VSVHAKAVPLNELLSDLCRQAGMKWRGESVTMPVDADFDDLPLDIALLRLLGGVNYSL
                     TWAPLPEDPGAASGRRYRPVEVWTGHRDAGERAPEDAGPEESWPSLESCLADLARPGS
                     TRRFARAARALAATGTPEAVRPLFETLRTMPAGEQRNELVHIAGSVTNPAAAAALAEI
                     WLTADADQPAERAASESLARVIDADGLRQLRAACERRSASVADSRFVDFVRRICSPAA
                     ESAVADWTGAEGRAPGTDLEKAAWSAMSRIGTGSAADRLIERADTVPMEEADFLYESV
                     EGMAPTPQALASLRYAAEGNKLATREDTRLAALEALSRFPDGESLALLQRLAEDPSPS
                     IRRAAGGILRAWGAPPPVFAPVEP"
     gene            complement(24498..26726)
                     /locus_tag="PK634_05495"
     CDS             complement(24498..26726)
                     /locus_tag="PK634_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOW97223.1"
                     /translation="MRKIMAFLMAAAVLMMASHASAGTISLSNGKTMSRLAGRAGTTA
                     YFKFTVPAGANRLQVKTWGGYGNCDIYLRQGSLPTFSRYHFASTGSKNTETITVNPAS
                     AGDWYLLVHARANCSGVSLVASYWTEGVLTVSAPAIQPAGGVFTSSVNVALSCATTGA
                     AILYTLDGSEPSPTSTVYAGVFTLKSSATVKARAYKTGMTASSTAAATFTVVSPPSGP
                     ALTTLGNGQIVSALYGSTGSQLYYKLAVPANSTQLVIGTWGGSGDGDLYVRRGAQPTL
                     KAYDYRPYAAGNNEHVVLSQPASGDWYILLYGRSSFSGMNLQAFYRTAGSATVAATPV
                     ITPAGGAFSGSVTFSLSCATAGATIRYTLDGTEPTASSAAYAGPYALKGSATVKAKAF
                     KSGLVNSATAVAVFTVNPSALTTLGNGQIVSALYGSTGSQLYYKLAVPANSTQLVIGT
                     WGGSGDGDLYVRRGAQPTLKAYDYRPYAAGNNEHVVLSQPASGDWYILLYGRSSFSGM
                     NLQAFYRTAGSATVAATPVITPAGGAFSGSVTFSLSCATAGATIRYTLDGTEPTASSA
                     AYAGPYALKGSATVKAKAFKSGLVNSATAVAVFTLNPASVSGVDLDRFSWKPTSANGT
                     TGNLTLPLRWETRRIGMSADYTSDYLGRLNPNSSGNYVVTIPARTKLIIQMHSTICPD
                     PSLPSHGMVLEFSFDPRYAQTLRTTQVTHYNYGSIYSTLRNWTWQLRSSSGGWNKYRL
                     VQ"
     gene            complement(27028..>27609)
                     /locus_tag="PK634_05500"
     CDS             complement(27028..>27609)
                     /locus_tag="PK634_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOW97224.1"
                     /translation="RCLCSRKSKNAQVETSFYGQLKPFAKWLLVPITAALSDEFDRSS
                     DAKAARQYLKDSGVDYRDRQTDENKGYGNSITVDLMGGVEADALRYTSIRGTVTEGLL
                     MISRINDFHKLYFEPRGHCLFFIYKDRPGILGRIGAALAQAGINIDDVRNPHDTKQDK
                     SIAILKVNCAVPGEVVRSIAESIEAFSSFHIEF"
BASE COUNT         4248 a         9567 c         9356 g         4438 t
ORIGIN      
        1 ccggcctcgg cgacgatctg cacggtgtcc ggcagggaca cgacccactc gcgcccgtcg
       61 ccggggacgt ggttgtagat gccggcggcg agggcccgca cgagatcgac gcggcccgag
      121 tagcggtcgc cgaggatcac ggtgatgccg gccgggatac cgaagccgcg gacgcggccg
      181 gcgttgggca catcgacctc gacggcgagc gggtcgtcga tcgccagcgg cgtcatctgc
      241 tcgtagtccg gcatgtcggt gtcgcccgcg cgggccagca gggcgccctc tccgacgaat
      301 cccacccagc cgcgcgtcgg cagcacctgg cggacctgat cggcgtcctc catgaggttg
      361 acgaagtcct cgacctcgcg ctggttcagg ttgcagaaga tcaggctcgc gttgacgacg
      421 ccgggcaggt cctcgaagaa gatttcgcgg gcgctatccg cggccacgca gccgtggcgg
      481 gacggcaggt ccacgtacag gcgggcctcg atgtactcgt ccgtcacgac cagcgaggtg
      541 cggggcagga tcgcctggcc gggggcatac agcgggatcc ggcggcgcga gatgccctcc
      601 ttgtcgtacc gcgcgaggct ctcgaggcgc gcggacagct tgcggacgag gaagtcctcc
      661 agcgcggtcc ggcggaccgg cgtgttcatg aggtgggcgg ggaatccggc gatgacctgg
      721 ggcacgcggg tgagcaccag cgtggactgg ccggcggccg cgtcctggat ctggttgatc
      781 ttgaggacat agcgggtgaa gtcgaaatcg ccgatcaact tggcgtattc ccggtactcc
      841 ttgccctcga tttccatcag caacccgtag aactcttcct tgtctctcat cgtcgtttcc
      901 ttttcagcta cggctttccc cgactcacga atcggtggcc acgcgaacca ggcccccccc
      961 cacatcgacg cgctggaggc cgtcgagcac ggtccgctcg cgctccatgc ccgagaacat
     1021 caactcgacg cgccggacca tccaatgcgc cgccacgccg ggcaacggca gatgccccca
     1081 ccgggcgccg cgcacatcga atgcgaaagg gcctccgccc gcgcgcggga gcccggcgat
     1141 ctcgtaggac atccgcagcg ggccccagag cgcgacgacg gtgacggtga ccccctcggg
     1201 tcggaaccgc aggttgatct cgccgatccc cagcttgctc aagccctccg aactggacaa
     1261 ctcgggatgc tccttgagga cttccgccag gtacgcgttc acctcggcct cggaaaaggt
     1321 ctgcacgacc atcaccctgc gctccacggc ctcggacagc aggcgcagtt tgccgtagta
     1381 ggccacggcc tcggcgcgcc cgcccacggc cccctggggt tttacaggcc agaggagcag
     1441 gcccaccccg gcgaggatca ggacctggac gatccgcgtg aggaagcggc cccagcgcac
     1501 cggctccctc cacgccttgc cgtccaggtt caacttggcg ccgcagttcg agcaaaaaag
     1561 gcgggtgggc tcgttttctt ttccgcagcg ggagcacgcg attttcatga agatcaaccg
     1621 gccttgcttt tcgtggtgtc gcccttggcg gcgcttcccg cggccggggc ggccttcgtc
     1681 gattcgcccg atttgtcggc gcgggccgct ttccggtacc cggcgctgcg gtaatccgtc
     1741 gcgtaaaagc ccgctccccg gaagaggatg ccggcgccgc ccccgggaag ccgctcgatc
     1801 ttcccgcggc accggggaca ccgctgcagc ggccgatcct tcatggcctg gaacttctcg
     1861 aaccggtggc cgcaccggcg gcactcgtac tcatacgtcg gcattgccca tgctccggaa
     1921 aacggaaaac ctctcgttcg aggacgccgg tcgcatcggc ccttgtcccc ttttcatccg
     1981 cttgtcgtgc gataatctag tgaggaggcg gcggtttgtc aattgcactg cgcccgtctg
     2041 ttcttaacac cgggcggccg gggtgatata gttaaaccat gttcgacgtc gaagcctaca
     2101 tgggggcgca ccggtcccgg atcgaaaagg agctcgaccg gtacctgccg cccgccgcga
     2161 cgcggcctgc cgtgctccac gaggccatgc gctacagcgt cttcgccggg ggcaagcggc
     2221 tccggccgat cctgtgcgtg gcgacctgcg aggcggtcgg cggcagcgcc gaaccggcgt
     2281 ggctgccggc cgcggcgctg gagctgatgc acacctacac gctcatccac gacgacctgc
     2341 cggccatgga caacgacgtg ctgcgccgcg ggaaaccgac ctgccacgtc gtgttcggcg
     2401 aggcgaacgc catcctggcc ggggacgcgc tgctgacgct ggcctttgaa tggatggcgc
     2461 gcagtctccc ccctccgccc taccagcccg gtcattacgt cctcgaattg gcggaggccg
     2521 ccgggagcct gggggtcatc ggggggcagg tggaggacct ggcggcggag gggcagccgc
     2581 ccacggcgga gctcgtcgag cacattcacc agcgcaagac cgcggcgctg atccgggcgt
     2641 cgatccgcat cggcgcggcg gcggggcgcg ccaccgagga ggagtttaac gcgctcacgc
     2701 tgtatggagg cgacatcggg ctggcctacc agatcacgga cgacatcctc gacgccacgg
     2761 ccacgacgga atcgctgggc aagacggcgg gcaaggacca ggcgcggagg aagtgcacct
     2821 gggtcgaggt tttcggcctg gaggcctcct ggcagaaagc ccatgccctg gccgaccgcg
     2881 ccatcgaggc cctcaagccg ctgcaccgga aaggcgaggc cctgaagtcc ctcgcccatt
     2941 tcctcgtcaa ccgggaatcc tagtccgccg ccccggccgc cgccgtcgga ccggcatccc
     3001 gtccttccgc tccggccctc cttccctccc cgcgcgatcc cgaaggctac gcaccttatg
     3061 cgcatatgcg cataaggtaa tcaggcggcc tgcaagcgcg cggccgggat cggcttcatg
     3121 gaatgatcgg gcgcgcggcg gcgcggcagg cgatcagaat acgacggtct tgttatggta
     3181 gaccaggatg cgcttctcga tgtgccagcg cacggcgcgg gccagcacca ccttctccag
     3241 gtcctggccc tgccgcacca ggtcgcccac ggtctgccgg tgcgagacgc gcacgacgtc
     3301 ctgctcgatg atcggcccct cgtccagggt ctccgtcaca tagtgcgcgg tggccccgat
     3361 gatcttcacg ccgcgcgcgt gcgcggagtg gtacggcttc gccccgggga atgccggcag
     3421 gaacgagtgg tggatgttga tgatctggtc cggatagcgc cgcacgaaat cgcccgaaag
     3481 aatctgcatg taccgggcca ggacaactgt gtccacgcct gccgactcca gcaaccgggt
     3541 ggcctgctcc tccaccgcgg cccgctcccc cggcgtcacg gacacgacgc ggaatgggat
     3601 cccgaaccgt tcggcggccg ggcgcagatc gggatggttg ctgacgatca tagggatgtc
     3661 cgcgtcgagt tcaccggcct catgacgcgc gagcaggtcg tacaggcaat gggactgctt
     3721 cgagacgaag atcgccaggc gcggccgccg gtcggagaag aacagctgcc agttcatgcg
     3781 ccgcggcccg gccagctgct ccacggcctc gcccagccgc tcccggggga tccggaagcc
     3841 gtccaacgcc cattccaccc gcgcgaaaaa aacgttctgc tcggtatcca catgctggtc
     3901 gagatgcagt atattgccgt tttcagacgc gatgaacccc gccagcgcgg ccacgatgcc
     3961 ccgctgatcg ggacagtgaa tcaacagaat ggcggtgttc ggcttcacgg actgctgcta
     4021 ttctaccgga tgggcaggga cgttcaagga gctacgcgca tgagcacggc ggatatcggg
     4081 ctggtcggac tcgcggtcat gggggagaac ctgatcctca acatggaaag ccgcggcttc
     4141 acggtggcct gctataaccg gacggcctcg aaggtggagg ccttcgtgca gggccgcgcc
     4201 cgcggcaaga aaatcatcgg ctgcgccacg ctcgaggatt tctgccgcgc cctccgccgg
     4261 ccccgcaagg tcatgctgat gatcaaggcc ggcgcggccg tcgacgccca gatcgaggcg
     4321 ctcctcccgc acctggaagc cggcgacgtg atcatcgacg gcggcaacag cctgttctcg
     4381 gattcgcggc ggcgcgcggc gttcctggcg gcccgcggcc ttctcttcgt cggcacgggc
     4441 gtgtccggcg gcgaggaggg cgcgctgacc gggcccagca tcatgcccgg cggacacgag
     4501 caggcgtggc cgctgctgcg gcccatcttc caggccatcg ccgcccgcgt ggacaacgcg
     4561 ccctgctgcg agtggatcgg ggccgacggc gctgggcact tcgtgaagat ggtccacaac
     4621 ggcatcgagt acggcgacat gcagatgatc gccgaggcct acgcgctgct caagggcgcg
     4681 ctggacctgc cggcggacga gctgtccgcc gtgttccgcg agtggaacgc cgacgaactc
     4741 aacagctacc tggtcgagat caccgcggac atcctggcca agaaggacga cgagaccggc
     4801 cgcccgctgc tcgacctgat cctggacagc gccgggcaga aagggaccgg caagtggacc
     4861 agccagaccg cgctggacct cggcgcggcc gcgccgacca tggcggaggc cgtgttcgcc
     4921 cgctgcctgt ccgccctcaa ggacgagcgc atggaggcct ccgcgcacct cgccggcccc
     4981 ggccggaaaa gcgtgggcca ccgcatcgcc ttcatcgagg tcgtccgccg ggccctctac
     5041 gccagcaaga tatgctccta cgcgcagggg ttccagatca tgcgcgcggc ggcggcggag
     5101 tacggctgga cgctgaacta cggggccatc gccgtgatct ggcgcggcgg ctgcatcatc
     5161 cgcgcgcagt tcctcggccg gatcaaggac gccttcgaga agcagccgga cctctcgaac
     5221 ctcctcgtgg ccccctactt ccagaaggcc ctcaagcgct gccagggcgg ctggcgccgg
     5281 gccgtgacgg cggcggtgaa actcggcatc cccgtcccgg ccttctccag cgccctcgcc
     5341 tactacgacg cgtaccggag cgagcggctg ccggccaatc tcctgcaggc ccagcgggat
     5401 tacttcggag cccacaccta tgaacgcatc gacaaaccgc gcggacagtt cttccatacc
     5461 aactggacgg ggcgcggcgg cacgaccgcc tcgacgacct acgaggtgtg aagccccgcg
     5521 gctcgcgctg gtcctggccg cgctcgcgct ggcggcggac gggcggacct ggacgagccg
     5581 atcgggctcg cagatcgagg ccgacctgga atccgtccag ggcgagttcg ccgtgctccg
     5641 ggccgccgac gggcgccagt tcaagatcca ggtcgcgcag ttgagcgacg cggaccaggc
     5701 gttcgtgcgc gagagcgcgg cgtcccccgt cccggccccg gccggcgagc cggcggcgac
     5761 ccccgctccg ccccccgccg ccccgcccgc agaatccgcg gggacggcgg acccggccac
     5821 ggcctggatc gccttcacag cgcccgatcc catgacctcg gacaagttcc ccggcctcgt
     5881 ggcgaccaac ctcccccgca tgatgctcgt cggcctccag tacggcccgg cgaagaccga
     5941 ggttctgtac tgcgcgtttg agacaaccga tccccagcag cctccgtccg cgctgcacct
     6001 ctacagcccg gcggcggagg gctacacgcg gacggtcacg ctccagggca cacgccggaa
     6061 ggtggaggaa tcccgcgtca cggggtacaa ggacgtgcgg ctcaaggcgg ccttcgggga
     6121 cgtccaggtg agcgcgacca tcgagttcat ctgcggaatc ggccgcagcg acacggtcct
     6181 gctgaatgcc gcggtccagc tcgcgcgcgg cggcgccgcg tcctcctgcc tgctcggcgg
     6241 gtacctgaac aacgatttcg tttacggggc gggaacgatc cccatcctgc cgctgctggg
     6301 caaaccctcc ctcgaggtga agacctggat gatgcacgga ccggtgctga cgggattcgg
     6361 acaggtgggt aagttgagcg tgatcccgaa gaccggcatg gcctcgaccc tcgccgtcga
     6421 ggtcaccgag cgcgagtcgg gccgcagcct cgaccggttg aagggggaat tcaacgagaa
     6481 gaagttctcg gccgggcaac cggaccgcac aatcaatgtt tccctccagc tcgaacaggg
     6541 caagaaagtc gccgtgaaat cggtgatgga cctcggcccg gcgctgggca aggccgaaaa
     6601 aacctcccat ttctattgat cccgccgggc cgttgccttg cggcccgcga agaattttgc
     6661 tatctgtaac gacgaacagg aagggaaaag cacatggcct cccgcatcaa agccgctatg
     6721 ttggcgatcg tcgggctgac cggccctggg ccggccatgg cgcaggacat cgtccccgcg
     6781 ccggaacccg atgtcgtcta cacgatcccc atccgcggga tgattgagcc cgccctgctc
     6841 tacgtcatcc ggcgcggcgt ccgggaggcc gaggccgccg acgcccgggc gatcgtgttc
     6901 gtcatggaca cgccgggcgg gaccgtggac gcggcgggcg aaatcgtccg gaccatcacg
     6961 ggcctggacg tcccctccta tgccttcgtc gagaaccagg cgttttcggc cggggcgatc
     7021 atcgcgctgg cgaccaagca catctacatg gcgcccggct cgatcatcgg cgacgcgatg
     7081 cccatcctct cgaccccgtt cggcggcgtg caggagatgt ccgaggacct caaggagaaa
     7141 tccgtcgccg ccgtggccgc gatcatccgg acggcggccg aggcgggcgg gcacgacaag
     7201 gaactcgccg agaagatggt gcggcgcgaa ttggagtata aggtcggcga cgaggtgatc
     7261 agcccggccg gccaactgct gacgctgacc aacgtcgagg ccgagcgcga ggtcggcgac
     7321 ccgccccgcc ccctgctctc cagcgggacg gtcgcggacc tggaagcgat gctgcgggag
     7381 gtgggcctcg ggaacgcggc ggtccgcagc ctggagatca cttccgccga gcgggtggcg
     7441 cgcttcatcg cggcgctggc gccgctcttc ctcatcggag ggctgctggg catctacatc
     7501 gagatcaaga cgccggggtt cgggctgccc ggcatcctgg gcatcatctc cctggccatc
     7561 ttcttctggg ggcaccacat cgccggcctg gccgggatgg aggaactgct gatcttcgcc
     7621 ctgggcgcgc tgctgctggc cgcggaattg ttcgtgctcc cgggcttcgg cgcggtcgga
     7681 ctcatcggca tcgcgctgat cctgtgggcg ctgctgggcg ccatgatcca gcgctatccc
     7741 ggcgggccct ggatgccggc ctggcagcag gtgcagttcc cgttgctcaa gctgtccctg
     7801 gccctgatcg gcacggcggc catcggcgcc ctgatcgccc ggctgctgcc gtcctcgccc
     7861 gtcttccgcc gcctcgtgct gtcccaggcc acgcggcgat cggagggctt cgcggcgtcg
     7921 gaggacaccc gctcgctggt cggccggacc gggcgggcgc tgaccccgct gcgcccggcc
     7981 ggcaccgcgg agttcggcga gcagcggatt gacgtggtca cgtccgggga atacattggc
     8041 gcgggttcgg acgtgcgcgt cgtggaaagc catggcaacc ggatcgtcgt agaggccgtc
     8101 ggcggcggcc cggcgagagg agtttgacta tggatcaaac catcctgatc tacatcatcc
     8161 tgctgctggc cggactgctc ctgatcggca tcgagatttt cgtcccgggc ggggtcatcg
     8221 gggcgctcgg cgcccttgcg ctgatcgtgg ccatgttcct cgggttcaag gcgttcgaac
     8281 cgccgggcgg catcatcagc gccctggcca tcgtcctcct caccgggctg ggcacggccc
     8341 tctggatccg cttcttcccg agaacttcgg cgggacgaca cctgaccctt tcccaggacg
     8401 ggcgtgattt caagtcggct cccccggaac tcaaggagtg ggtcggccgg gagggcatcg
     8461 cgcacagcac gctccggccc ggcgggatcg ccctcatcga cgggcaccgg gtggacgtga
     8521 tcgccgaggg ccgctggatc gagagcggcc gccgcatccg cgtgctgaac gtcgagggga
     8581 cgcgcgtgat cgtacgggac atcgcggacc aggaaaaaac ctcatgaacc ttcgcctccc
     8641 ttcgacgctc gcgcttctcc tgctcgccgc gtccgcgggc gccgccccgt tgagtttcga
     8701 ggcgcccgag gagccgtttg tcctggacga ggagcaggcc ctcaccaacg agttatggct
     8761 ggtcgccccg gccgcggata tccgcggggc ggtgcaggac gacctgttcg cggtcggcgg
     8821 aacgctgctc gtggccggcg ccatgggccg cgatgtctgg gccctcggca acgaggtcga
     8881 cctgaccggg cgcgcggccg gccacgcgcg gctgctgggc cgcgccctcg acatccgcgg
     8941 ctcggtcgcc ggcaacctga tcgggcttgg cgggtccgtc ggcctggccg ccgacagcga
     9001 ggtgggcggc gacgcggtgc tggtcggcga ggacgtgacc gtcgagggcc ggatcggggg
     9061 gcgcgcgcag atctacgcgc agcgggccac gctgagcgga cggttcggcg gcacggtccg
     9121 cctgtacgcg aacgatatcg tggtgcatcc cggcacggag atccgcggcg acctggagta
     9181 cacgtcgtcg cgcgacctga tcctcgacga acgggtcgcg ctgacgggcc agctgatccg
     9241 gaaggaggcg gcggcccccg ccctccgctc gacgccggcg agctggagcg aggcgctctc
     9301 catccagctg ctcctgttct ccgcggccct gctggtggcc ctgccgttcc tggcggtgtt
     9361 cccgggctac gcgggccggg cggtccgcct gctgcggcag tcgggatgga aatgcgcgct
     9421 ggccggggtg gccgccttcg cgctgttccc catggcggcg ctcattctcc cgctgaccat
     9481 cgtcggcatc ccgctggccg tggtcctgct gctgctgttc atcctgctgc tctacgcgag
     9541 caagttcatc ctggcgctgg cggtcggcgg atggctcctg cggcggcgcg gggcgctgtc
     9601 gttcgggcgg gcgatatcca cgctggccgt ggggctcgcg ctgctctacg gcctggtcaa
     9661 cgtgccgggg atgggcctgg ccatcgggct gatcatcctg tggctgggcc tgggcagcct
     9721 ggtcctggcg gccctggcgc cgccgtccga cggggccgcc gagcccccgc ccctgccgga
     9781 gccggcgggc gggccgccgc ccgttccacc tttcccggga agcaagcaac cctgaataaa
     9841 ggagtcgtgt catgctgacg caagtggtca ccgtgttgtt cgtcctgctc gccatcgcgg
     9901 tcctggtcct gatcggcctg ttcttctact tcctgaaggt ctggatccgc gcggcgatgt
     9961 ccggcgcccg ggtcagcctc ttcaacctcg tcgggatgaa gctgcgccgc gtgccgccca
    10021 ccctgatcgt cgatgcgcgc atccgcatgg tgaaggccgg gatggacctg caaacggatc
    10081 cgctggaggc ccactacctg gccggcggca acgtgatcaa cgtcgtccag gccctgatcg
    10141 ccgccgacaa ggccaacatt gacttgagct tccagcgcgc ggcggccatt gacctcgccg
    10201 gccgcgacgt ctacgacgcc gtgcgcacga gcgtcaatcc gaaggtcatc gactgccccg
    10261 acccggccaa gggcacgacc atgctcgacg ccgtggccaa ggacggcatc cgcctgctcg
    10321 tcaaggcccg cgtgaccgtg cgcgcgaacc tcgagcgcct cgtcggcggc gcgaccgagg
    10381 acaccattat cgcccgcgtc ggccagggca tcgtcagcgc catcggctcc tcgctctcct
    10441 ataaggacgt gctggagaac ccggaccaca tcagccgcaa ggtgctggac agcggcttgg
    10501 attcccagac cgccttcgag atcgtatcca tcgacatcgc ggacatcagc gtcgccggca
    10561 cgggcgcgcg ggacgtcgcc aacgtcggcg ccatgcttga gaccgagcgc gccgaggcgg
    10621 acaagaagct gcgccaggcc gaggccgagg gccgccgggc catggccatc gcggccgagc
    10681 aggagatgcg cgcccgcgtg caggagatgc aggccaaggt cgtcgaggcc caggccgaga
    10741 ttccgcaggc catcggccac gccatgcgcg ccggcaagct gggggtcatg gatctcatgc
    10801 gcatgcagaa catcatggcg gacacctcga tgcgggagtc gctcgccgag ggcgggcacg
    10861 agacgccgaa atcctgacgg ggccgttcgt aaagaggagc gcgcgggtga aacatctgtt
    10921 ggcggacagc gcgtacgaga gcctgttctg gctggtgatg gcgatcgtct gggtcgtcgt
    10981 gcaggtcatc gcccgcgcca cgaagcagca gctgcgcaag ggcgccgcgc cgcgcccgac
    11041 gccggccgcc gccaaatcgg aatccagcct ggaggaattc ctccgctcgc tgggggccga
    11101 gccgctgctg ccgcccaagc cggcgcagga gctggagccc gtgccggaag ccgcgcccga
    11161 gccggagccc gccccggcca tccggatacg caagcgcagg gccccgcgac ccgctgagcc
    11221 catcgcgccg gcggcggaga ctccgccccc gcccgccgcc ctgccccgcc tggcgcccga
    11281 ggcctgcgtc atggcggagg gccggcggat gcgcgggatg atggtcatcc cgaagatccc
    11341 cgtggtccgg gtgcagggcc tccgggtgcc gtcgctgtcc ttcaccggaa cgaccgtcaa
    11401 caatatccgg cccgggccgg atcggttccc gctgaaaggg cgcgaggccc tgcgcctggc
    11461 gatgcttcac cgcctgatcc tcgagccgcc gcacaacatc catccattgt aacatcccgc
    11521 cccgcagcag gatgcgcatc cgctcctccc tcttcaggaa gaatcaaacc gatccttcga
    11581 ctgaagagtg cattcggcac gagtaacggc gccgcgcggc ccgagccgcg aacgagacaa
    11641 ccgccccgtg tccgatgaac acaacaaagg gacaacgatg cactcgaatc gatggcggcg
    11701 gacgatcggt tatctctgcg cggtattgac cgcgggcgga atttcgaaag cgacggccca
    11761 ggagttgatc gcatgggaca gcgcggccga tccggtctat gacggctcct ggtgcgatcg
    11821 ccaggaggga ggcgggggct ggaccacgcc ctggatgctg tggacccgcg acaccggcag
    11881 ttgcggatac ttcctgcaga cctccacgtc caacggcgat ggcgaccgga cgggggacgg
    11941 ggacatcgac accaacggcc gggcctgggg cctgttcgcg cgggacggcg tcacggcctg
    12001 ggcgctgcgg ggattcgccc cgctcacgcc ctgggacacc ctgtgcctct ccatggacaa
    12061 cggctggatc gacggcgacg gcgtcgtggg gatggggttg cagacctccg actaccagaa
    12121 tcgcgtcgaa ttctacttcg tggccggcat ggcccactac atccttcacg acggcgacgg
    12181 gccccgcgac acgggcatcc cgttcacgga cgaaggcctg cggctgctct tctccatcgt
    12241 ggacggccag cgctatatcc tgacggtcat cgagagggag accggcatcg cccgcacgtt
    12301 ctccgggcgg ctctcgggcg aggcgaatgc ggccatcgaa ctggtccgtt tcttcaacta
    12361 caacgccaac gcgttcggcg ccgacggcgc gcctcgcgat ctctatgtca acagcctcgc
    12421 cctctacacg atgcggccgg acgcggacga gcgcgccccg cagatcgaac tcctggggtc
    12481 ctccatggtg acgatcgagt gcgggaccgc cttcgccgac cccggcgccc gggcgttcga
    12541 cgacatggac ggggatctgt ccgatcggat cgcgacgagc agcgacctga acgtccgccg
    12601 gcccggcgcg tacaccgtga cctaccgcgt cgcggacgcg gcgggcaacg agggcgtcgc
    12661 cacgcggacg gtgcgggtgg tggacaccat cgcgcccacc ctctcgcgtg tgtccgacca
    12721 gaccgtggtt ccaagcacca gcgccgtgct ggccccggta ttctttcggg ctccgacggc
    12781 gacggacaac tcggactccg tgagcgtggt ctgcgtgccc gcgcccggga gcttcctggc
    12841 gccgggcgca cacacggtca cttgcaccgc caccgattcc tcgggcaacc gaagccagtt
    12901 cgttttccgc gtaacggtcc tgcccttcct gcgcgtagct ttccaagcgc cgatccagga
    12961 cgataacaag gcggacgaca tcgagaagga cgccgaccag gcgaatctct tccgagtcgg
    13021 cgtcccgatg ccgcacatcg tcaaactcta cgactgcaag ggccgcgatg tcacggccgc
    13081 cgtagcgccc ctggtgaagc tccagctcaa cgtcacggag cgcgagcaac ggggcgcggg
    13141 ctcggtgcag gtcgcggatc cgccggagaa ggcttcggtg gtgggcggcg cgaagggggc
    13201 catggcgccg gtcggcgaca cgttccagtt caatctcgat acgcagacat tcgaggccgg
    13261 gacgcaaaac aacgcccggt tcttccgatc cctcgtccgc gcggcctacg tcgccgtgcc
    13321 gggagtcttc tttgaggagg acgccatgct ggagagccgc tgacgaacag cccgggaagc
    13381 ccagccgaca ttcgcgggca tggaggcggc cgcttgccaa acggtcgcct tcaccctata
    13441 aacggggctg ctcgcaaaag ccggagtggc ggaatggcag acgcactgga ctcaaaatcc
    13501 agcgccttta cgggcgtgag ggttcaactc cctcctccgg catatcctgc aaacaatgat
    13561 tgaaagcgtc ttggtcaaca aggacaggtc cgttctgaac accccgcctc ggcgtacctc
    13621 cgctcgccga gcaacacgaa atcgtccgcc gcgcgctgcg tgcgtccagc gctatctctt
    13681 cagcagcccg cggatcgccc gggcgtccgc gaaaccgcgc gccacgcaac cgaccaaccg
    13741 gtaggaggtc agcaggctgg ggatggcctc gcaggcgttc cagcagatcg ggcactggcc
    13801 ccggtcgatc aggcggcggg tgtcccgcac gagttcgccg cggagcagcg cggcgatgcc
    13861 gtccttgaaa tgcccgagct tgcggctcca cacgatgcac ggatagagga agccgtacgg
    13921 gtccatgtac agcgagttgc gcgtggcgga gcagtgcatg accagcttcg tcggctggtc
    13981 caggaacccg gggatctccc ggaggaagaa cacgcggtac atgctcttca gccgctgcag
    14041 gaggcgcagg tcgtcctcct tcaggcgcgc cagggcatac tcgatgtcgt tccgggccag
    14101 cgcgcggaac tcctcctccg acacccggcc gacggccttg tccgggctga acgccgtctg
    14161 gtagagcccg ccggtatgct ccagcccgaa ccccacgtcg cccatgttca gcccgagcgg
    14221 gcgcagcaca tcccggtcga aggtctccag cttcccggcg ttgcggtagg agatcgtgta
    14281 gctgatcttc gcctcgacgt tggggcgcgt ttcggacagc ttgcgcaggc ccgccagggt
    14341 gctgacggcc gtttcgtagg cgtcgtcgcg cccgcggatg aagttgtggt tgtcccgcgt
    14401 gccgtccagg ctgacgccga tggccacgat ctgcctcggc atggcccgcg cgaccttctc
    14461 gacgctctcg acgatgcggt cggtcaggag cccgttggtg gtgatgacga cggacgacaa
    14521 cctggggcag atgcgcgcgg cgtcgcccag gatgtcgggc atgtcctggc gcaggaacgg
    14581 ctccccgccg gtccagtcca ggtacagcag gctggtccgg agttgctcga agatgttctg
    14641 gaaatccgcg cgggtgagtt cctcgtccag cttcgccttg gccttgcggt acacctgcca
    14701 gatgtcgcac atcttgcaac ggtggttgca gaggaacgtc gaggccacgg tcagcttcat
    14761 gggcagcagt ttctcgcgca ggaacggaac gcgataccgc atgaaacgct tgccgagttc
    14821 ggtgtacata ctcatactgg ccagccggtt gcctctcatg cgcagtatac gtcaaagacg
    14881 ctcgcgcgca atggccattc gggagccggg cccatgagcg cgcccgccga ccgggtcgaa
    14941 cgctggcgcc aggacgccct gtcggtgcgg accggaacgg gcgcgcaccc gaaggatttc
    15001 gactggctgg cgaagagcat cccctgccag gccgcctgcc cggcccgaac cgacgtcccg
    15061 gcgtacctcg acgccatcgc gcagggccgc tttgacgagg cctaccgcat caacctggcg
    15121 gacaacgtgt tcccggcggt gctgggccgc gtctgcacgc gcccctgcga gcccgcctgc
    15181 cggcacggct gggaagaatt gggaagcccg gtggccatct gcttctccaa gcggtccgcc
    15241 gcggacttcc aggaccgccg ggagccggtc acgctggacc cggttttcgg gcccagcggg
    15301 aaaagcgtgg ccgtggtcgg cggcgggccc gccggcctgt cggccgcccg cgagttggcg
    15361 cggtggggac accgggtgac cgtctacgag cgcgagagcg agccgggcgg cctgctggtc
    15421 cagggcatcc cccggttccg cctgccccgg gaggcggtgg cgcgcgagat tgaacaggtc
    15481 cgcctgtgcg gggtccgtat cctgtgcagc cagccggtcg gcgccgatcc ctcgctcgat
    15541 accctgctct cgtccttcga cgccgtgatc gtggccgcgg gcgcgcaacg cctccacacc
    15601 ctgcccctgc ccggttccga gttggggggc ctccggcacg ggctcgcttt cctgcgcgag
    15661 atgaatgccg ggggccgatc gaagccgggc gaccgcgtgg tcgtgatcgg cggcgggttc
    15721 acggcggtgg actgcgcgcg cgcggcccgc cggctgggcg cctcctcggt ccgcgtcctc
    15781 taccggcgct ccgaagagga aatgcacatc acgcccggcg agatccgcga aatgaaggac
    15841 gaaggcatct cgttcgaaac gcaggcggcg ccggtgtcct tttccgggag cgggggccgt
    15901 atcgaaagcg cgcggttcat ccggacgcgc atacaggacg gcccgggcgg ggggcggcgg
    15961 gatcccctcc ccgtccccgg cagcgagttc gacatcgcgt gcgacgccgt gctcctggcg
    16021 atcgggcagg cccccgacac agcctggctc gcgggccgga ccgagccccg gctgttcacg
    16081 gcgggcgact ttgcgtccgg ctcggattcc ctgattcacg ccatcgccga cggcaaggcg
    16141 gcggcccgcc ggctggacct gttcctcatg ggcgccgcac gcctgcgcga cgtcgcggag
    16201 atcgtccacg cgcgccgcac cggccgcacg cgggcgatgg acgagatccc gcggcaggag
    16261 atgcccgtcc tgccgctccc ggcccggttg ccggaggcgg aagtcgagac gggattcgac
    16321 cgggcggcgg ctcaacgcga ggcgcagcgc tgttacctgt gccactacaa gttcgagatc
    16381 gacaacgact actgcatcta ctgcgatcgc tgcctgaaag caaagccgct gcccgactgc
    16441 atcgtcaagg tgagccgcct ggtccacgac gagcaggacc gcatcgcggg cttcatcccg
    16501 tccaccgggc ccgaggatta ccggatgctc tacatcgatt cctcgaagtg catccgctgc
    16561 ggggcgtgcc gcgacgtgtg ccccatggcc tgcatctccc tccagcgcgc aagctggcgc
    16621 gccgcgaacg catgaccgac gccaccccat ccgggccggg cccgaaaagg ctcgtggagg
    16681 cgggcatcgc cctcctcgtt gtggccgccg cactcctcct cgccaccccc gcgtggcgtc
    16741 ccccgaagct ctttcccgac tccctgacct acatccagtg ggacccgatc cggccgccgg
    16801 cctactgcgc cttcgtcgcc ctcttcgggc agggcgccgc gctcgtccag gtccagagcc
    16861 tgctgtcgct gctctcgtgg gcattcgccg ggtggatgct ggggcggggc gcgggccttt
    16921 tcctgctgac gcttctcgcc atgagcttgc cggtggccca atggaacctc atgctgctgt
    16981 ccgagtcctt ctcgtactcg ctggtcatgc tggccatcgg cctgaccctg cggttgctcc
    17041 ggaagtggtc ctggggcggg ttcgccgcct gggtcgctgt cgccgcgctc ctgtcgttcg
    17101 cgcgcctttc caatctcttc atcacgccct tcctggcgct tcccttcctg gcccgcgccc
    17161 ggacggcatg gcgggccatg ggtgcggctt cgctggccct gttcctcgcg gggctatgga
    17221 tgagcgcgac gcgcggggcg gacttcgagc gcatggaatt aagcaacgtg atcatgggcc
    17281 gcatccttcc cgacccggag gcgcgggcgt ttttccacgc gcgcgggatg ccgctggacg
    17341 agcgggtgat gtcctacagc ggacgcctgc gaagccgcgc ttaccgcgag ctctgggcgc
    17401 gggcgccgga gttcagggcc tggctcacgc cggaagggat gcggacctac cgggcctggc
    17461 tgctgcgcga cggcgccgcg ttccgggccg cctggcgggc gctggcggaa ggcgacagcg
    17521 gcatcgggaa ttgcctgggg tacggcgggg acgtggcccc gcgcccggtc gccgcgcggc
    17581 tgctcccgtt ctacctgtgg ctcgggaaac tcccgcccgc gctgtggctg gccctgatgc
    17641 tgcttctccc cctgctcgat cgttggcggg acggcccgct gtctcctcgc tcgctcctcc
    17701 tgccggcgct cgcgacggcc gcctatgccc aggcctttat ctgcttccac ggcgatgcgg
    17761 acgaggtgcc ccgccacatg ctgatcgtcg gactcctgta tcgcgtgacg atcctcgccg
    17821 gcctgtgcgg cgcggtccgg ctggcggccg gcctgttcgc ggggcggaag cgggaagcgg
    17881 ccccgcttcc gccatccggc caagcatcag cgcaggaatt cgccgtacaa gaccgatgac
    17941 ggcaaggaag gctgcatttc ctcgaatacc gagggatgct ccagcagcca gcgtgcgtac
    18001 tcggaaaggg acggcggcag ttcgtacgcg ccgatgtcca ccgccggacc ggagatccgg
    18061 gccttgccct ggatgtccgt ctctccgggc cacacgagac tggagtcgcc cgcgttgcgg
    18121 cagggggagg cgctggagag cacgacgccg ttgccgaaca gcgggtcggc cgccatgttg
    18181 ccggcgcccc agaccgcctt gcctcccgtc caggcatgca cgcccgccgg gccattctga
    18241 acgttcgacg aatccacgta cacggtcgcg ccggcggtgt agatctggtt gctgtaggcg
    18301 tcgtcgttgc cccagaagat ggagttggcg atgccgtggt ggaggtaata gtaattctga
    18361 tagggccagc cgatgttcat ggtatagtgc acggccccgc cgccctgggc ggcgacgttc
    18421 tcgcggaagg tgcaattgcg cagttcggac ttctccgggt acttgtaccg ctgcccgcgg
    18481 aatcgctcgg gcagcgggat gttcagttcc ttgatcgccg cgccgccgcc ccacaccgca
    18541 aagttttggg cgaagaacga gttatccacg tgggggatgc cgtccacgac cagcatccct
    18601 ccgccgcagc cctcggccaa gccggacgga tcggccgcac cgttccagac ccaggcgcag
    18661 cggtagatgt ccagcggcgg gatgccgggc agttcgttgg tgggcgccag ccggccgatg
    18721 ctcgccaggc cgccgcccat gagcgcgccg ttcgccatga aaatgcaatc ctgcaggacc
    18781 gcggcgctgt cggtcagcat cacgccgccg ccctgcccgg tggccccgtt ctggccgaag
    18841 aaggacgtcg atatcttcgg gaaactcgcc agggccagca cgcccccccc gttcaggttg
    18901 ggtatcggcg ccgtgagcgg catgtggccg atgcggtcat attttccgca tgtgttctcg
    18961 acgacgatcg ccttgtgcaa cgcgggccgg ctgtcgtccc acatcgccaa cgcgccgccg
    19021 ccgccccaag cctcgttccg ataaaaaacg caatccgtca cgaaggggga actgtgcttc
    19081 agcgcgacgc cggcgccata accccgcgcg ccgcaaccgc tgaacgtcga gttgatgatg
    19141 accccgccgc tgttctccat gaggagcccc ccgccggcgc actggtccgg ggactgcccc
    19201 ttcgcctcgg cctggccatg tacctggcat tgggaaagat acaacgagcc atccgacacg
    19261 aaaacgccgc cgcccttgcc ggcgccgccg tcgatcgcgc tctgaacgag ggtcgcctgg
    19321 gaacctccca gcagcgccac cccgccgccc ttcagcgtcg agccgcacgc gttcaccacg
    19381 gagttctcca tcagcagccg gctgctggag agcagggcaa tgccgccgcc gacgcccgcc
    19441 ggcgtcgccg cgatcgtttc ccggatttcg aaatcgcgga tttccacctc gctgcccttt
    19501 tcgacgagga tggcgccccc ctcgttgtcc gcgctcgggt cggcggccaa tagggaggtc
    19561 ctgtagatga tgaggtcttc cagctcgact tcggcgtttt cgatccagaa cgccctgtag
    19621 gttccgaaaa tgccgaagat aagcgcctcg cctgccggat ccttggccaa ctggccgcag
    19681 atcaccggtt ttttccactg gtgaagccgg cgggaatcgg ccaactgggg aaaggctctc
    19741 gtgaactgtt ccggaatcgc gatcaggtcg ccggacacga tgcccttgat gaacagtttc
    19801 ttgccgctga tcttggaatt gaggttctcg gggccatagt agaagcccgg atggatttca
    19861 accgtgtccc cgtccttcgc cgcctcgaca gcgtcccgga tggtcagata gtcgcccgtg
    19921 ccgtctatct tcactttgag ggtcttcgcc ccggccgatg gcggcgcgag caggcacccc
    19981 gccatgagca gcagcaggag gacgcccacg tgccgacacg ccgcttccgt ccggttttcc
    20041 cgacgcggcc tggagttccg atgcatgggt tgctccttcc tgggttgggt tgcgttcgac
    20101 tttccgatgc tatcccgatg cccggcgcac ctgctcaaaa gagcgagtaa tcaggccttc
    20161 gggacggtaa agaatgtacg gagaaggcgg acggctgtat agctgaatcg tataagcatt
    20221 tcgtttcgcc cgaggaaatg ggcttccttt ttctttgtgc cgggcggctt gaagactatt
    20281 attaattatg gaacatcgtt tgccggccgc atgggagaac cgacgatgac gcagatcgcc
    20341 tgcccgattg aggaagcgct ggcgcgcaag tatcccgagc ccgtggtgct ggtaaccacg
    20401 cgcgaccacg acggccgggc caacgtcatg gcggccgcct gggtgtgctt cgcgtccagc
    20461 gagccgtgga tgttcctgct gggcatcgac gccggggccc gcacgtacga ggtcatcgtc
    20521 cggacccggc agttcgtggt cgccttcccc cacgagggca tgggccggga aacgctctac
    20581 gccggcacga accacgggca cgcgacggat aagttcaagg agaccgggct ggccatgcag
    20641 gacgcctcgg tcgtccgcgc ccccctggtc cgcgacgccg tcgccaattt cgagtgcgag
    20701 ctggtggaga tctaccagcc cggcgactgc cctctcatcg ccggcaaggt cgtcgcggcg
    20761 cacgcaaacc gcgacgccga tctccgccga ctctgtctcg tcggcccgga ttaccgcctc
    20821 ggcgggttgc ggcccgcgtg aagggccgag gccggaaaca cgcacacggc ggctgacaat
    20881 acggcccgcg ggagctacac tgccgggcca ctgcgcggga tgccatggtc ataggctggg
    20941 acatcaagtt tcgaacggcg tggccgtact atatggccca gagcctggcc gccacggccg
    21001 tcgtctttct cgtcctcctc gtcctgcaca ggaccaacgc cgtggtcgtc gcctccatcg
    21061 gatccagcgt gtacatcctg ttcgcgttgc ccgagagccc gacggccggg ccccgacggg
    21121 tgatcggcgg gcatgccgcc gggctgctgt gcggagggtt gtgggcgctg gtgccgcaca
    21181 cctcgcccga gggctgcgcc gccgtttacg cgctggccat cggcagttcc ctgttcctga
    21241 tggtggtcac cgacacggag catcccccgg cctccggaac ggcgctgggc gtcgccataa
    21301 cgggggccag cctggccctg ggcctttccc tgatggtcag cgtgacgctg ctttcgctcg
    21361 cccatcgcct gctgcgggga cgcctcaaga acctgctgta gcgaacgagc gccgcgcttc
    21421 gcgggttggc tttgcctcgc cgtttctcta gacttcgcct ccatgcggat cttgctgatc
    21481 acgccgcccc tgctgcagtt caacacgccc tacccggccg cgccggcgct ggcggcgttc
    21541 ctgaaatcca ggggccttca cgtcgcgcag gcggaccttt cgctcgaact gatccttcgg
    21601 ctgttctcgc gccggggcgt cctggacgtc gcccgggtca tgcggcgcgg gaatcgccct
    21661 tccacccggc acttcctcgc ccatgcccgc gagtacgccg cgacggtggg cgcggcggcc
    21721 gcctttctgc aggggcgccg cccgggcctg gcgcggcgca tcgcctcccg gcgattcctg
    21781 ccggagggcc cccggttcgc gcaggccgat ccgtttgagc tggaccgctt tcccctgccg
    21841 gagcgggcgc ggcacctggc cagcctgtac gtggacgacc tggccgacgc gatccgcgac
    21901 ggcgtcgatc cgcggttcgg tttggcgcgc tacgcggaga gactggccgt gcgcgcgccg
    21961 tccttcgcgc ccatacggaa agccctcgaa ggcccgccca cccgggtgga ccggctgatc
    22021 gaccaaatcg cccgggagct gatccggcgg cgccggccgg acctggtcgg gatcaccgtc
    22081 cccttccccg gcgcggtgta cggcgcgttc cggatcgcgc gggcggcccg gcggatgcgc
    22141 ccgcggccgc gcgtcgtgct cggcggaggc tacgtcagca ccgaactgcg cgacctgtcc
    22201 gatccgcggg tattcgacta cgcggactac atggtcctgg acgacggcga ggcgccgctg
    22261 ctcgcgctgg cggagcattt ggaagggcgc cggccctccg gacgcctcat ccggaccctc
    22321 gcgcgggcgg gcggacgggt cgtgcgccac gacgacagcg agccggacgt cccgcaccgc
    22381 gacaagccgg cgccggatta cgggggcctg aagctggacc gctacctctc catggccgag
    22441 acgctcaacc ccatgcaccg gctctggtcc gacgggcgct ggaacaagct gatgctcgcg
    22501 cacggctgct actggcggcg ctgcgctttc tgcgacacga cgctcgatta catccaccgg
    22561 ttcgatcccg ccccggcgtt ccgcctcgcg aaccgcatcg agcaggtccg ccggcaaacc
    22621 ggaagccgcg atttccattt caccgacgaa gcggccccgc cggcgctcct gcgcgcgctg
    22681 gcggaggagc tggtccggcg gcgcatccgg atccgctggt ggtgcaacat ccggttcgaa
    22741 aagaacttca cacccggcct ggcgcgcctg ctcgcgcggt cggggtgcat cgcggtcagc
    22801 ggcgggctgg aagccgcgac ggaccgactg ctgcggctca tgaacaaggg catcacggtg
    22861 gcgcaggcgc ggcgcgtgat gaaggcgttt tcggaagccg ggatcatggt ccatgcgtac
    22921 ctgatgtacg gctttcccac gcagaccgcg cgggaaatcc tggaagccct cgacaccgtg
    22981 cggaggctgt tcaaggcggg ctgcctgcac tccgcctact ggcatcgttt cgccctgacc
    23041 gcgcacagcc cggtcgcgca ggacccggcc gcgtacggca tccgcctggt ccccgcgcgt
    23101 cgcggcggct tcgcaaggaa tgagctgtcc tatacggaac cgaagggctt cgatcacgac
    23161 cggttcggcc ccgggctgcg caaggccgtt tacaacttca tgcacggcat cggcttcgac
    23221 gcggccgttc ggtcatggtt cgaccggggc gaataccggg ggcggagcgc cccacgcccg
    23281 tagaatgcct cctgcggccc ttcggatcga tggcgacgga tcttcggcca ggcgttgcag
    23341 caaggctagt gattcgccgt cggggaaacg ggacagggcc tccagggccg cgaggcgggt
    23401 gtcctcgcga gtagccagct tgttgccttc cgcggcgtac cggagggagg ccagcgcctg
    23461 gggcgtgggg gccatgcctt ccacgctttc atacaggaag tccgcctcct ccatcggcac
    23521 ggtatcggcg cgctcgatga gccggtccgc ggccgagccg gtgccgatcc gcgacatcgc
    23581 cgaccacgcc gctttttcca ggtccgtccc gggcgcccgc ccctcggcgc cggtccagtc
    23641 cgcgacggcg ctttccgccg ccggcgaaca gatgcgccgg acgaagtcca cgaagcgcga
    23701 atccgcgacc gaagccgagc gccgctcgca ggccgcccgc agttgccgca gcccatccgc
    23761 atcgatgacc ctcgccaggg attccgaggc cgctctttcc gcgggctgat ccgcatcggc
    23821 ggtcagccat atttccgcga gcgccgcggc ggccgcggga ttcgtgacgc tccccgcgat
    23881 gtggaccagt tcattccgct gctcgccggc cggcatggtg cgcagggtct cgaacagcgg
    23941 ccgcacggcc tcgggcgtgc ccgtggcggc cagcgcccgc gccgcccggg cgaaccggcg
    24001 cgtggacccc ggccgggcca gatcggcgag acaggactcc agggagggcc aggattcctc
    24061 cggcccggcg tcttccgggg cccgctcgcc ggcgtcccgg tgtccggtcc agacctcgac
    24121 cgggcgatag cggcgcccgg atgcggcgcc cggatcttcc ggcagggggg cccacgtgag
    24181 cgagtagttg acgccgccca gcagccggag aagggcgatg tccagcggca ggtcgtcgaa
    24241 gtccgcgtcc accggcatcg tcacggactc gccgcgccac ttcatgccgg cctggcggca
    24301 cagatccgag aggagttcgt tcagcgggac ggcctttgcg tggacggaca cgcccgagga
    24361 ttggacgatc acgcgggggc gggcggccgc ccggccatgg gattccggaa atgccgagcc
    24421 gcacagcgcg gcgacgacca ggcccagccc cagcgcgccg cgaagatcat tccgcccgga
    24481 cacgtccggc ccccgcccta ctggaccagg cggtacttgt tccagccgcc ggagctgctg
    24541 cgaagctgcc aggtccagtt gcgcagcgtg gaatagatgc tcccgtaatt gtagtgcgtc
    24601 acctgggtgg tgcgcagggt ctgcgcgtag cggggatcga aactgaactc caggaccatc
    24661 ccgtgcgagg gcagggacgg atcggggcag atggtgctgt gcatctggat gatcagcttc
    24721 gtgcgggccg ggatggtcac cacgtagttg ccgctggagt tcgggttgag ccggccgagg
    24781 taatccgagg tgtagtcggc gctcatgccg atgcgcctgg tttcccagcg cagcggcagc
    24841 gtgaggttgc ccgtcgtgcc gttggcgctg gtgggcttcc aggaaaagcg gtcgaggtcc
    24901 accccgctga cgctcgcggg gttcagggtg aagacggcga ccgccgtcgc gctgttcacc
    24961 aggccgctct tgaaggcctt ggccttcacc gtcgcgctgc ccttcagcgc gtacggcccc
    25021 gcgtacgccg ccgaggacgc ggtgggctcc gtgccatcca gcgtgtaccg gatggtcgcg
    25081 ccggccgtgg cgcacgagag ggaaaaggtc accgaacccg agaacgcgcc gcccgccggc
    25141 gtgatcaccg gggtggcggc cacggtcgcc gagcccgccg tccggtagaa cgcctgcagg
    25201 ttcatgcccg aaaaactgct ccggccatag agaaggatgt accagtcgcc ggaggcgggc
    25261 tgggagagca cgacgtgctc gttgttgccg gcggcatacg gccggtagtc gtacgccttc
    25321 agcgtgggct gggcgccccg ccgcacgtag aggtccccgt ccccgctccc gccccaggtc
    25381 ccgatcacca gctgggtgct gttggccggc accgcgagct tgtagtagag ttgcgaaccc
    25441 gtggatccgt acagcgcgga aacgatctgg ccgttgccca gcgttgtgag ggccgacgga
    25501 ttcaccgtga agacggcgac cgccgtcgcg ctgttcacca ggccgctctt gaaggccttg
    25561 gccttcaccg tcgcgctgcc cttcagcgcg tacggccccg cgtacgccgc cgaggacgcg
    25621 gtgggctccg tgccatccag cgtgtaccgg atggtcgcgc cggccgtggc gcacgagagg
    25681 gaaaaggtca ccgaacccga gaacgcgccg cccgccggcg tgatcaccgg ggtggcggcc
    25741 acggtcgccg agcccgccgt ccggtagaac gcctgcaggt tcatgcccga aaaactgctc
    25801 cggccataga gaaggatgta ccagtcgccg gaggcgggct gggagagcac gacgtgctcg
    25861 ttgttgccgg cggcatacgg ccggtagtcg tacgccttca gcgtgggctg ggcgccccgc
    25921 cgcacgtaga ggtccccgtc cccgctcccg ccccaggtcc cgatcaccag ctgggtgctg
    25981 ttggccggca ccgcgagctt gtagtagagt tgcgaacccg tggatccgta cagcgcggat
    26041 acgatctggc cgttgcccag cgtcgtgagg gccgggccgc tcggcgggct gacgacggtg
    26101 aaggtcgccg cggccgtcga actggccgtc atgcccgtct tataggcccg ggccttcacc
    26161 gtggcgctgg atttcaacgt gaagacgccg gcgtacaccg tggaggtcgg cgagggttcg
    26221 gagccgtcca gggtatagag gatggccgcc ccggttgtcg cgcaggacag ggccacgttt
    26281 accgagctag tgaacacgcc cccggcgggt tgaatcgccg gcgcggagac ggtcagcacg
    26341 ccctcggtcc agtacgaagc gaccagggag acgccggagc agttcgcgcg ggcatgaaca
    26401 agcaggtacc agtcgccggc ggaagccggg ttgaccgtga tggtctcggt gttcttcgaa
    26461 ccggtggaag caaagtgata gcggctgaag gtcggcagcg agccctgtcg gaggtagatg
    26521 tcgcaattgc cgtagccgcc ccaggtcttg acctggagcc ggttcgcgcc cgccggcacc
    26581 gtgaacttga aataggccgt cgtccccgct ctgccggcca gcctcgacat ggtcttcccg
    26641 ttggaaagcg aaatggtgcc ggccgacgca tgggacgcca tcatcaggac ggcggccgcc
    26701 atcaggaagg ccatgatctt gcgcatgcgt ccggctcctt tcgccttgcg atcatccatc
    26761 gttttttccc cgcattgccc cttgcctata ccctgctgtt agtaaaatgt taataaccat
    26821 gtttaccggc tattccgacg gcagccatcc gcgcgaaaat cacgacgtct aatatcctgt
    26881 ccaaacacaa gttgcaccga gcgcaagata gcgcagccgt actgacaatc gcaacttggc
    26941 aaaaatgccc tggcggggtc aagttttttc gcggcgcaag gcggagctcc ggaccaacag
    27001 gcggccgagg gcatgagcgg gcgccggtca gaactcgatg tggaaggaac tgaaggcttc
    27061 gatgctttcg gcgatggagc gcaccacttc gccgggcacg gcacagttga ccttcaggat
    27121 cgcgatggat ttgtcctgct tcgtgtcgtg cgggttgcgc acgtcgtcga tgttgatgcc
    27181 ggcctgcgcc agggccgccc cgatccggcc gaggatgccc gggcgatcct tgtagataaa
    27241 gaacaggcag tgcccccggg gctcgaagta gagcttgtgg aagtcgttga tgcgcgaaat
    27301 catcagcagg ccttccgtca ccgtgccccg tatgctggtg tagcgcaggg cgtccgcctc
    27361 caccccgccc atgagatcca ccgtgatgga attgccgtag cccttgttct cgtcggtctg
    27421 ccggtcgcgg tagtccacgc cggaatcctt gaggtactgc cgggccgcct tggcgtcgga
    27481 cgagcgatcg aactcgtcgc tcaacgccgc cgtgatcggc acgagcagcc acttggcgaa
    27541 gggcttgagc tgcccgtaga aactggtttc cacctgagca ttctttgatt tcctggagca
    27601 caggcatcg
//
LOCUS       DAOOTK010000031        27121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1257_length_27121_cov_4.344528, whole genome shotgun sequence.
ACCESSION   DAOOTK010000031 DAOOTK010000000
VERSION     DAOOTK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27121
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_27121_cov_4.344528"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK634_05505"
     CDS             complement(<1..354)
                     /locus_tag="PK634_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOW97225.1"
                     /translation="MPDQPSGFLLPQPGERVHLLGIGGVGMAGLAVHLDGRGARVSGC
                     DAGRNRLTDDLERRGIRCVVGHDPAHLEPDVKQVVRSTAVPDSCEEPRERTGMPRARA
                     ARSSSQESGTAVLRTT"
     gene            complement(347..1438)
                     /gene="murG"
                     /locus_tag="PK634_05510"
     CDS             complement(347..1438)
                     /gene="murG"
                     /locus_tag="PK634_05510"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOW97226.1"
                     /translation="MKTAIACGGTGGHIFPGLATAELLRQRGHDIELWLAGKDVETPA
                     LRGWAGPVVTVPAEGLPSGFTPRAVRSVWKLARAARLCRRQMRQRRPDVLLAMGSYAS
                     VGPVYAALREAVPVVLHEANVLPGRAIRLFSRWADAVAVSFEESSYYLRHGELVWTGM
                     PLRAELEQAATAAEQAPSEREFFTVLVMGGSRGAHRLNEIVSKAVSRVHAAGHRVGVI
                     HLAGFADEDAVRRAYEQAGVLHEVFPFMADMAAVYRRADLAVCRSGAATCAELAIFGV
                     PALLVPYPFAAYDHQTANARALEKKGAADVVPEPDLDEEWLADYLVSAMRNPGRLARQ
                     RAAARQRASARGAEALAGLVEKVAEARRA"
     gene            complement(1476..2027)
                     /locus_tag="PK634_05515"
     CDS             complement(1476..2027)
                     /locus_tag="PK634_05515"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOW97227.1"
                     /translation="MSRTLEQPGELRMESTWGVVRVEVRRGRICACVLPALRAEPGQP
                     FRWKTAQVHAATADDRAALAKARRFILSVFRGQPGKPPALEWPETAPFTLRVWRALLT
                     LAPGTVVGYGELARKVGRAGSARAVGRACGANPLPLFIPCHRVRAGDGSLGGFSGGLP
                     WKRLLLDREARAARRSAPRARHP"
     gene            complement(2030..2548)
                     /locus_tag="PK634_05520"
     CDS             complement(2030..2548)
                     /locus_tag="PK634_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97228.1"
                     /translation="MKTQFERRMKELERESRRVRADLRTLTKALDKPERLATVPRPQS
                     LVARERPVAPPRREDPVTSPEEAAPPSGGAVADAPPPGELFAWRSPAAARPASPRAPE
                     PAAPRAGRMVEPTRKAGHLLKDDRFANYFASGSFVGAPPVKGEGRVQRNKAVFMIFIV
                     VIVGFVLYSLIF"
     gene            complement(2576..3322)
                     /locus_tag="PK634_05525"
     CDS             complement(2576..3322)
                     /locus_tag="PK634_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97229.1"
                     /translation="MADTDFFDDDLIKQRDSAKRIKLGPGDEPAVPMGEPASDEVPVR
                     PITDFNLTRMARFKQDMGERSAQASQDIERLRQRQEEIEREKHDMDDLRRKTEEYERG
                     KRDMLERFGQSLVTLEKEELQAEKLSELLAAVRKRFKMMAAELQELNEETWPDESYRD
                     ELTRALSILENARQEYNKSMSRLDAIQGEKGAPAEHPQVTFEDHRGFVDVERSFGYWI
                     KVGLGVSLPLIVFILVLVAVFIVLRSSLLI"
     gene            complement(3348..4076)
                     /locus_tag="PK634_05530"
     CDS             complement(3348..4076)
                     /locus_tag="PK634_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOW97230.1"
                     /translation="MRKGMRWIGGLGLVLLMAAGGCSRSAGSLDQMEERDPLLKRARE
                     RKNQQDVDGAIELFNKALERKPNLARAHLEVGHLYDSQKQDYIRALYHYQRYLELRPQ
                     TEKRPVVEDLIRHARVRFASTLPAQPSGAIEEIALLRQEIEALRARLGEAAKAVPAAA
                     AAATAAAAPAAAQVAGAPRPPEPAPAQPPMSTYIVQPGDNLSRIAERMYGNSGRWSDI
                     YEANRSTLKSPESVRVGQTLVIPK"
     gene            complement(4185..6455)
                     /locus_tag="PK634_05535"
     CDS             complement(4185..6455)
                     /locus_tag="PK634_05535"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOW97231.1"
                     /translation="MKLRSVLFSLLLLSAGGCSRSGPEQVLSTAELIRRWADPLQAAR
                     LDVPDTTLLSSVDPSGKNDDYNHPLRKGPEGWVVLADLEGPGYMSRFWFTGAENGQHR
                     IRLYFDGERKPSVDTTLDGFCGGADPWRHPLAAYENYCWFNLVPIPFRKRLVVMVQEG
                     GFKPGGWPRLFFQINFSRLPPGMTTEEFRGTYSPEVVAEILRVRDAWVAGGLAPVPDG
                     CEARESTLTLQPGEGADGLDVAGPAMIRQLTVTPDYGPIGDAVAREKLGRQLALQVRW
                     NDSDAPSVETPLGDFFGQVWRRVRFPSMFFTLTNDSYVSRFPMPFEKRARVRIENQSG
                     QPVTVSVRADVQSLPAWEAGWGYLHAHWSRTTAQDVGKPHPILLAEGAGKYVGCILAV
                     MSEDRSWWILEGDEQMYADGAAAPKWRGTGLEDYFNGGWYYQNVIARPLHGVPFKSFF
                     RVVQYRLHLLDATKFGKSFRMDFERGPDQASRGAMESVAWYYLAAPAAAGSALGTALG
                     RTPPRDPLAEATVMNELLNYERFGDYRGAREYIEAFLQMHSKFPKAALLRLRELAYRE
                     RLEGFDVVRPEYERFAAEETDPEAVEQAKLLLWFHEDPSHVLVSLSSTAPARALLDGQ
                     EAVTAGRPERAVVKGATAGPGRHALALHTLWSPYPSWLQAAVRTHEGLVVTSPDWKYK
                     FRPAESWGATDYDDSGWPAVGGTGTKGPPEEPFIWMEPNGFVDLQSQAVGLRANDEVW
                     PDKRGFFVFRRVFERP"
     gene            6628..7104
                     /locus_tag="PK634_05540"
     CDS             6628..7104
                     /locus_tag="PK634_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97232.1"
                     /translation="MSGKREDECCPEFDPGPWDDKELEWRDRLFVRDRVRSFLHIPLN
                     FGAVMKRNMIRIQAADAEPAAAAVLSDENSLWGADVYIEVTKEIPGAPMARISGTFLS
                     RVFEGPYRNMGAWIKAMQRHVKEKGRTLRKLYFYYTTCPKCAKKHGKNYVVLLAQV"
     gene            complement(7101..7559)
                     /locus_tag="PK634_05545"
     CDS             complement(7101..7559)
                     /locus_tag="PK634_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOW97233.1"
                     /translation="MKEIEFIARGVLVAGGKVLVCRNRKKGNCYLPGGHIEWNESAPV
                     ALAREMKEETGLACRVGRFLGAVEHTFRHKGERVCEINLVFELRLPSRRPGAPVPSTE
                     EKLEFFWWPIRRLDDIAVEPSPLRRCLDAWRKKPGAASWASTYGRGRRAG"
     gene            complement(7556..8905)
                     /locus_tag="PK634_05550"
     CDS             complement(7556..8905)
                     /locus_tag="PK634_05550"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOW97234.1"
                     /translation="MTKDSPAAPFLDRLYRRSAHGIKMGLEAMEGLAARLDHPERAFV
                     CVHVAGTNGKGSVCAIMERALRAAGWVTGLYTSPHLVRFNERIRVGGAAVSDADLAGL
                     IRRVDAADRDQVAAGGREATFFEFSTAMAFEHFRRSGVQIAVLETGLGGRLDATNIVR
                     PLLSVITRIGIEHTEYLGTTLEQIAGEKAGIVKPGVPVVLGAMPAEARGVIERTARER
                     RAPCVAAEEAVNVRSPRATLDGQSIQVETAGAVYGPLILPLPGAHQLENAATAVAALE
                     YLGSLSSFSCDEAAIRSGLERVWWPARFQVLSRDPAVILDGAHNPDAARVLADTLKRV
                     LGRRELALVVGLLADKDLAGFMRELTPRVKRAWAVQIRNERTLPLDRLVAGIRAAGLE
                     VEGLPLRKALRQAREWAAQRDGAVCVAGSLFLAGEVFETMGIHDLDVLCREAEKEES"
     gene            complement(8940..9827)
                     /gene="accD"
                     /locus_tag="PK634_05555"
     CDS             complement(8940..9827)
                     /gene="accD"
                     /locus_tag="PK634_05555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOW97235.1"
                     /translation="MAFLRKRNYSTVKVRKRDMPDGLWLKCPSCNEIIFKQELEAALN
                     VCPKCGHHFQIGRRQRLDLLIEPGSFEEWDAQLYSVDTLGFTGVAPYLEKLEENRKKT
                     GCRDAMLCGRARIGAHRAAIGVMDFNFLGASMGSVVGEMVTRLIERATRERLPALISC
                     ASGGARMYEGMLSLMQMAKTSGALARHAEAGLPYISLLTHPTTAGVMASFATLGDVIV
                     AEPGALIGFAGPRVIKEATQQDLPAGFQRAEFLLQHGLLDMVVPRKDLKAAIVRLLDF
                     MMGRPAGAPAPKPAAPAEA"
     gene            complement(9849..10577)
                     /locus_tag="PK634_05560"
     CDS             complement(9849..10577)
                     /locus_tag="PK634_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOW97236.1"
                     /translation="MSLSWKAAAARWLDMAYPRWCAGCGGPTADAEGFLCWNCLSAIP
                     FVQLPFCARCGDPIEGRVDHAYTCYTCTADEIHFERARSAVRYRGVAQQVLRAFKYDA
                     ALWVQSDLDRLLHGCVESQFTDLEADAVAAVPLYAARERERGFNQAALLAAALARRLR
                     KPFLSGVLRRIRPTPTQTHLTAPQRAANVKGAFEVRKPVWIEGQRLLLVDDVMTTGAT
                     VNECARVLKEGGAARVYVATLARG"
     gene            10670..11479
                     /locus_tag="PK634_05565"
     CDS             10670..11479
                     /locus_tag="PK634_05565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOW97237.1"
                     /translation="MNTREFAGHSIVRNLAGGGMTRLFVALDSAQNRVVLRYLLENWA
                     RKWRFRKRFFHGAKVLAKLDNPHIVKILKTGYEGRIPYMTMEFVESRTLRDLILYREP
                     LLTQNVLSLMRQMAAALFYIHAQGYLHLDWKPENILVRPDGHIVIIDFDLAMKRKRRP
                     IRVKELPGTPSYIAPEALTRHLVDERSDIYSLGVTFYEMLTYHKPFEGDKIEQVRAAQ
                     IDPNTPPTRPRQYNPDVPVAMESLVLKCLAKQPDDRYPSLSLVIRDLEALL"
     gene            11476..12447
                     /locus_tag="PK634_05570"
     CDS             11476..12447
                     /locus_tag="PK634_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOW97238.1"
                     /translation="MKDLYLRPRRSTRGCLIAVILLLLVILAAWWFWRRPKAEEPAPD
                     SPAAIPATAAAPAPVPAAKEPPPAPEAKAPAPKAPVASRPSPAAADPGLDLLARAREL
                     RASDKLVEARDAAWQALAQSSNEVARTGAEALLNEVNIELLLSPRMMPEKEEYVVASG
                     DSLDKLRKKFGTTVEVIRRSNRIPGDVIRAGDRFRIFKGRFTIQVSKSRNDLVLMMND
                     RFFKRYRVGTGEYGKTPTGDFTIKEKIAQPTWWRPDGKPIPFGDPENVLGTHWLSLDI
                     PGYGIHGTWEPDTIGKQASAGCIRLLNAEVEELFNLVPEGTPVRIVE"
     gene            12468..13466
                     /locus_tag="PK634_05575"
     CDS             12468..13466
                     /locus_tag="PK634_05575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOW97239.1"
                     /translation="MPPYILPFEKPILDMEKKLEDLRAFSQAQNIDVSFEIERMQNKI
                     TEMRREIYENLGAWDKVLVARHPQRPYMLDYLAAMTTDFTELHGDRVHADDRAIIGGF
                     GWLEGRRVMFIGTQKGRDTKSNLERNFGCAYPEGYRKALRLMKLAAKFGAPIVTLIDT
                     PGAYPGIESEERHIAEAIAVNLRDMFRLPVPIVVVVIGEGGSGGALGIGVGDRVLILE
                     YSYYSVISPEGCAAILWKNRSYANRAAEALKLTAPDLLKAGLVDEVLPEPPGGAHADP
                     AAMSATLKAALLRNLEELDATPVEQLLEQRYNKFRAMGVFGEDAAPAPAASPAPAS"
     gene            complement(13468..>14074)
                     /locus_tag="PK634_05580"
     CDS             complement(13468..>14074)
                     /locus_tag="PK634_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97240.1"
                     /translation="ILLDIQLPEMDGYAVAGDRNSIYFGVQLDMAQWLSQHGGRASTL
                     CIRETRHVPGNLTAWRTGYQYFNLPMSMGDDRCVYTNTMTLEAVSPVTAVGRWFHLPN
                     HAMVHAAAQWVVAGGSPGVDVRLRAYALGADGRQQQVAESFHPGVNQGGGQSAVEALS
                     LWLPPGYYRLEMSRTTGSCAGFGYTARMYVRYDPEGSVLYY"
     assembly_gap    14075..14084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14085..14779)
                     /locus_tag="PK634_05585"
     CDS             complement(<14085..14779)
                     /locus_tag="PK634_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97241.1"
                     /translation="MKRIEAFLKIALWMLAGMAVVARGQIPPKPDLPGALPRYQWCLV
                     EAPMEYVQYAPYETRTWTFRHPGATSMKVRFESTGLMIKSAGYPGENSRIEILDGRDR
                     VVQILTGSAGAFDTVSVTGGVIKVRYLNNANGGGGTLFAGAGDRFRVAGYKYYRSILV
                     SEGGAGALGFQVMSPLPLPAQPLKTYRYNACEIPLAVKKPFNWGAQDGARYKVDFFLD
                     GIFLGSSAGYAVAG"
     gene            15098..16066
                     /locus_tag="PK634_05590"
     CDS             15098..16066
                     /locus_tag="PK634_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOW97242.1"
                     /translation="MAQRAGVSIATVSNVLKGRMSEVSAATAERVQRVVEKMGYVKNL
                     TAAALSSQRSGLVGAVLSNIFSASPSQPDVEINPFYGEFLLLLDRELQPTGSTLCLYT
                     GREETFVNFLLERNLDGAILVGIVAKELPPVLERRESRLVFFDSFLQHRVYMRVQTDE
                     LKGGGMAADYLIAQGRKHLVFVGGAVRDLPSNIPAVRFEGASRVCDQSGLRLMLIEES
                     TSFEGGRRAARRVLDLKADGVVAAADVMAAGLIRGLQEAGAKVPDDVAVVGYDDLPLA
                     RMISPPLTTVRQGLPEKVQAAVELLKDGEKGEVRIIKPHLVVRESA"
     gene            complement(16058..17857)
                     /gene="aspS"
                     /locus_tag="PK634_05595"
     CDS             complement(16058..17857)
                     /gene="aspS"
                     /locus_tag="PK634_05595"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOW97243.1"
                     /translation="MMRSHTCGELRKSHVGQRVQLCGWVDTVRDHGGVIFIDLRDRYG
                     VTQVIFDPRDSQEAWDRAQGSRGEYVIRIEGTVEPRPADMANPKLATGEIEVRSNTIT
                     VLNRSATPPFPLDDEEAAKVSEDLRMAYRYLDLRRPQMQKNMRYRHEMVQAIRVYLDR
                     AGFTDIETPMMTKSTPEGARDYLVPSRILPGTFYALPQSPQQYKQLLMLAGFDRYYQI
                     ARCFRDEDLRADRQPEFTQIDMELSFVTPEDIYGIIDGLLAIIMKAAGHGEIALPLPR
                     MKYDDAMNRFGSDKPDVRFGMEMTDVSDVFAGTQLKVFAGVLAKGGVVKALNAKGQGN
                     TSLSVMDDWTAMAKDAGLGGLAYIRVSPEGEWKSPIAKFLSEGERQGLTKKLGMQPGD
                     LVMFGADKLATVNTALGRIRLIAGEAAGLIDPKKFAFTWVTDFPLFERNDEGELQAMH
                     HPFTSPHAEDLAFLDSDPLRVRAQAYDIVLNGVELGGGSIRIHDPELQARMFKILGIP
                     TAEIEDRFGHLIRAFGYGAPPHGGIALGLDRLIMLMVGGESIRDVIAFPKTQKAMDLM
                     MNAPARVDAKQLRDVHIRLDLPVENPATGPASG"
     gene            complement(17862..19136)
                     /gene="hisS"
                     /locus_tag="PK634_05600"
     CDS             complement(17862..19136)
                     /gene="hisS"
                     /locus_tag="PK634_05600"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOW97244.1"
                     /translation="MTVPSFQPLQGMSDLASPEVHLWQEVEEAARRLLALYQVEEVRT
                     PILEPTGLFVRSLGEGTDVVQKEMYTFEDRGGRSVSLRPEGTAGVIRYLAGLGPEAAG
                     ARLYYLGPMFRAERPQAGRRRQFHQLGVELIGPPGPAQDVEAIALQQHLLNAWGLKAA
                     RIEINSRGLPAEQAAVSEGLRKALEPHEPRLCDDCRRRLKANVLRVLDCKQEGCRAVA
                     ETLPAVTDFMGADSRAYLDEVRRLLDLLELPAVINPRLVRGLDYYQHTIWEIRHPALG
                     AQDALAGGGRYRIEWEGAAIEGVGFAAGLERIVTAVQHDRPASAESARGKPATWIVSQ
                     GAKALEQNLVLAQLLRQRGIACGLALDGRSVKAQMRAANRSGAAFAVIRGELEMEKGL
                     FVLKSMKDGAQEELELPALVERLLRASRAEGT"
     gene            complement(19156..19437)
                     /locus_tag="PK634_05605"
     CDS             complement(19156..19437)
                     /locus_tag="PK634_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOW97245.1"
                     /translation="MTKRELVVRIADETGLIQQDVFAVIQKTLDYIIEALKKGDTIEF
                     RNFGVFEVRERKSRIGRNPNRPTQVVTIPARKVVKFKPGRIMKQLITGA"
     gene            complement(19571..19801)
                     /locus_tag="PK634_05610"
     CDS             complement(19571..19801)
                     /locus_tag="PK634_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97246.1"
                     /translation="MARRYHVQGSKHFLIAAIVLMLLGLWCVRDGWFPSEAVRAEKTA
                     HELASFMQFNKSLALLLLVGSVICGYIHTVVR"
     gene            complement(19824..21557)
                     /locus_tag="PK634_05615"
     CDS             complement(19824..21557)
                     /locus_tag="PK634_05615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOW97247.1"
                     /translation="MRWSRQLIPTLREVPQEAEIPSHQLMLRAGLIRKLGAGLYTFLP
                     LGLRALRKVERIVREEMNRAGALEILMPALHPKEVWEQTGRYELLRDVMFKVKDRQQR
                     EMVLGPTHEEIVTDLVAHEVSSYRQLPVNLYQIQTKFRDEIRPRFGLMRAKEFIMKDG
                     YSFDADLDGAEKSYRSMYEAYQRVFQRCGLRAKIVEADTGAMGGSSSHEFMVLADSGE
                     DGLVECDACSYAANLERAESVLRSAPSFADAEKDPEKIATPGLRTVEEVAGFFQSPPA
                     RLIKTLIYVADGKPVAALVPGNRDVNEIKLRRALGAKALELADAATIARVTGAPVGFA
                     GPVGLSIPLVVDAKLRGYRGAVTGANEADAHLRSVDIERDVKVTEFADISFAIEGDGC
                     PRCAGTLRAKRGVEVGHVFKLGTKYSEQLGALYLGEDGQKRPCVMGCYGIGVTRTLQA
                     VIEQSHDDNGIIWPATIAPAEVEVLLINEQHAASVETAGKLVEGLKNVGIEVLFDERA
                     ERPGVKFKDADLLGLPLRINVGERGLAKGSIELRARHSGETLVVPVESAVETACARLA
                     ELKRACAPGGE"
     gene            21731..22147
                     /locus_tag="PK634_05620"
     CDS             21731..22147
                     /locus_tag="PK634_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOW97248.1"
                     /translation="MKKKIYLCGPIMDEHEGLARAWREEAKKRFGQDFILLDPMRRNF
                     KDREVDSANEIVEFDLQDIRDADILLVNYSKNSVGTSMEVFFASQNLGKFVVAFSPYT
                     FKDCSPWMVRFCTKILPNLETATDYIREHFLVQHMA"
     gene            complement(22172..22324)
                     /locus_tag="PK634_05625"
     CDS             complement(22172..22324)
                     /locus_tag="PK634_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOW97249.1"
                     /translation="MSLHSSWKTGSKIAAKRNVLKRFERIESLKKGGKWKDGDRAYGL
                     PKTKGT"
     gene            complement(22406..23020)
                     /locus_tag="PK634_05630"
     CDS             complement(22406..23020)
                     /locus_tag="PK634_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97250.1"
                     /translation="MIWTSATNGEESLVGELRAEVARVAPDADEASDDVARGVAAFWR
                     ERYDRQWLSSDYVEHLIAAAWRGVENPGGPGSPARSLEAWRGLASRLYRSAEWAGGDR
                     VWILDLGKVGGLPCYRLELLWFPVVRRLIEDMAELWDATGGHGVLGVVGGVPAGISGR
                     ALRRWRADLAGHGEAVLARLRRQRGWAELPRLLWMDPVSGHAGD"
     gene            complement(23017..23472)
                     /gene="nrdR"
                     /locus_tag="PK634_05635"
     CDS             complement(23017..23472)
                     /gene="nrdR"
                     /locus_tag="PK634_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOW97251.1"
                     /translation="MRCPKCGQQEDKVIDSRSVRNGDVIRRRRVCLGCSHRFTTYEEV
                     IKAHLRVIKRDGRHEDFDRRKLVNGVTRACEKRPISTQQIETLVDGIAGELESEYERE
                     VPSTVIGKKVMDRLEKLDEVAYVRFASVYRRFRDVNQFLSEVEGLIGRQ"
     gene            23565..24026
                     /locus_tag="PK634_05640"
     CDS             23565..24026
                     /locus_tag="PK634_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97252.1"
                     /translation="MERKWRSCHFSGDRNRHFRYEFCWISGLVKSRFHEEEFTSSPLS
                     SWRVSSPLSSWLPLLSPPLHPRALPSRDWLTAGAAYAIPLTRVNPPRRASIFSANLSS
                     APPPHKGADSRQDLPFVLPILRDGDRSAADLSPTSSKKEQDAVNALHRLEK"
     gene            complement(24045..24710)
                     /locus_tag="PK634_05645"
     CDS             complement(24045..24710)
                     /locus_tag="PK634_05645"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOW97253.1"
                     /translation="MKILVTAGPTREAIDPVRFISNRSSGKMGYAIAAAAAHRGHEVT
                     LVSGPVSLQPAANMNVVNVVTADQMRLAVRRHIRWCDALVMSAAVADWRPAATAVKKI
                     KKRNRRALSLKLVPTVDILRDVRALKGRRIYVGFAAETGAPEKDARRKRKEKGLDLIV
                     ANNVSRQDSGFETDTNRVILLSADGAREAWPLMTKARVGVRLVRWIEQQMLVRLKKVH
                     DKR"
     gene            complement(24707..25243)
                     /locus_tag="PK634_05650"
     CDS             complement(24707..25243)
                     /locus_tag="PK634_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOW97254.1"
                     /translation="MGKRVVLGVTGSIAAYKAAELVRRFTAKSWDVWVMMTEAATKYV
                     GPLTFHALTGHPVAAGKCEATPASVFEHLDLASCDALAIAPCTANVIAKLACGLADDI
                     VTATALACTAPLVVAPAMNEKMWKHPATQANLATLRQRGVKVMDVGHGELACGVVGAG
                     RLADVDAIVAEVSRALGA"
     gene            complement(25250..25870)
                     /gene="gmk"
                     /locus_tag="PK634_05655"
     CDS             complement(25250..25870)
                     /gene="gmk"
                     /locus_tag="PK634_05655"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOW97255.1"
                     /translation="MITLPRHPQVFIVSAPSGAGKTTLCDKLYAEFPSFFYTITSTTR
                     APREGEVDGKNYYFLTQEQFQRKLAEGEFLEHAIVHGNLYGSPKTPVVKALGEGRDVL
                     MNLDVQGAAAVREYARRAPEGDPMRIEVVDIFVVPPSLEVLRNRLTKRGKDTDETIAR
                     RLKQAENEIARWTEYKYMVVNDDLAEAYDNLRAIILAEHHLIRGGK"
     gene            complement(25867..26736)
                     /locus_tag="PK634_05660"
     CDS             complement(25867..26736)
                     /locus_tag="PK634_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOW97256.1"
                     /translation="MTGHGRGQAARGGISAEVEVNSVNRKQLDCSVSLPPVLGALAPR
                     VEEDIRGVLSRGRVGVTVGVQWSAAWRRKSLRVDEDLAEACVHALRKTARRIGLRGDF
                     SPDVLLQLPEVVRFEPAAREADQAWPVIREALRLALGGLVRMRGREGAVLRRDIEKRL
                     KSMEADLSAVRARAPEVVKQYRAKLAARLRDAGFDKEAADERVLRELALFADRADIAE
                     ELTRLASHIGQARRMLRDGGAAGRSLDFLAQEMLREAATIGSKANDAAIARRVVNLKA
                     ELERLREQVQNIE"
     gene            complement(26748..>27121)
                     /locus_tag="PK634_05665"
     CDS             complement(26748..>27121)
                     /locus_tag="PK634_05665"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433990.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOW97257.1"
                     /translation="VGVLQGTRTWVLQDSEGQIRLTHSVSAGLDYAAVGPEHALLRDL
                     KRADYSHVTDREAVDAFYTLARWEGILGALESVHAVAWLMKNARRWGKKDLILVNLSG
                     RGDKDVQQVSAWSAKHGGPKA"
BASE COUNT         4290 a         9562 c         8791 g         4468 t
ORIGIN      
        1 ggtggtgcgc agcacggccg tgccggattc ctgcgaggag ctgcgggcgg cgcgggcgcg
       61 gggcatgccc gttctttccc gcggctcctc gcaggaatcc ggcacggccg tgctgcgcac
      121 cacctgtttc acatccggtt ccaggtgggc cggatcatgc ccgacgacgc accggatgcc
      181 ccttcgctcg aggtcatccg tgagccggtt gcgcccggcg tcgcagccgg agacccgcgc
      241 gccacggccg tccagatgca cggccagccc ggccatgccg acgccgccga tgcccagcag
      301 gtgcacacgc tcgcccggct gcggcagcaa gaacccggat ggctggtcag gcacggcggg
      361 cctccgcgac cttctcgacc aggccggcga gggcctcggc gccgcgggcg gacgcccgct
      421 ggcgggccgc ggctcgctga cgggccaacc ggccgggatt gcgcatcgcg ctgaccaggt
      481 agtcggccag ccattcctcg tcaaggtccg gctcgggcac gacatcggcc gcgcctttct
      541 tctccagcgc ccgcgcgttg gccgtctggt gatcgtaagc cgcgaacggg tacggcacga
      601 gcagcgcggg gacgccgaaa atggccagtt cggcgcacgt ggccgcgccg gaacgacaca
      661 cggcgagatc ggcccggcgg tacaccgcgg ccatgtccgc catgaagggg aagacctcgt
      721 ggagcacgcc ggcctgttcg taggcgcggc gcacggcgtc ttcgtccgcg aagccggcca
      781 ggtgaatcac gccgacgcgg tggcccgccg catggacgcg cgaaacggcc ttcgagacga
      841 tctcgttgag ccggtgcgcg ccgcggctgc cgcccatgac cagaacggtg aagaactcgc
      901 gctcggacgg cgcttgctcc gcggcggtcg ccgcctgctc cagttcggcc cgcagcggca
      961 tgccggtcca cacgagttcg ccgtgccgca ggtagtagct cgactcctcg aaactgacgg
     1021 ccacggcgtc cgcccatctc gagaagagcc ggatggcgcg gccgggcagg acgttggcct
     1081 cgtgcaggac caccgggacc gcctcgcgca aggccgcgta gaccggtccg acggacgcgt
     1141 agctgcccat ggccaggagg acatcggggc ggcgctgccg catctggcgc cggcacagcc
     1201 gcgccgcgcg ggccagtttc cacaccgagc ggaccgcgcg cggcgtgaag ccggaaggca
     1261 gcccctcggc ggggacggtc accaccgggc cggcccaacc gcggagggcc ggggtttcca
     1321 cgtccttgcc ggccagccag agttcgatgt catggccgcg ctgccggagc aactcggccg
     1381 tggccagccc cgggaagata tgcccgccgg tgccgccgca ggcgatggcc gttttcaccg
     1441 ggcccgctcc cgtaaactag tccgcagaaa gcggatcatg gatgtcgcgc ccggggcgcc
     1501 gaccggcggg cggcccgagc ctcccggtcc agcagcagcc gcttccaggg caggccgccg
     1561 gaaaagccgc ccaagctccc gtcgccggcc cgcacgcggt ggcagggaat gaaaaggggc
     1621 aacgggttgg cgccgcaggc cctgcccacg gcccgcgcgc tgcccgcgcg gccgaccttc
     1681 cgcgccagtt cgccgtaccc gacgaccgtt cccggcgcga gcgtcagcaa cgcgcgccac
     1741 acccgcagcg tgaagggcgc cgtctcgggc cactccagcg ccggcggctt cccgggctgt
     1801 cccctgaaaa cggaaagaat gaaacgcctg gccttcgcca gcgccgcgcg atcgtccgcc
     1861 gtcgcggcat ggacctgcgc cgttttccat cggaaaggct gtcccggctc ggcgcgcagg
     1921 gcgggcagga cgcaggcgca aatccgcccg cggcggacct cgacgcgcac tacgccccac
     1981 gtcgattcca tccgcaattc gccgggctgt tccaaggtcc tggacatggc tagaagatca
     2041 ggctgtacag cacgaaaccg acaatgacca cgatgaagat catgaacacg gccttgttgc
     2101 gctggacccg cccctcgccc ttgacgggcg gcgccccgac aaaactgccg ctggcaaagt
     2161 aattggcgaa ccggtcgtct ttcaacaggt gccccgcctt gcgcgtgggc tccaccatcc
     2221 ggcctgcgcg cggcgcggcc ggctccggcg cgcgcgggct ggccgggcgc gcggccgccg
     2281 gggatcgcca cgcgaacaac tcgcccgggg gcggcgcgtc cgcgaccgct ccgccggacg
     2341 gaggggcggc ctcctcgggc gacgtcacgg gatcttcgcg gcgggggggc gccacgggcc
     2401 tttcccgcgc cacgagggac tggggacgag gcacggtcgc cagccgttcg ggcttgtcca
     2461 gcgccttcgt cagcgtccgg aggtcggccc gaacccgacg cgattcgcgc tcgagttcct
     2521 tcatccggcg ctcgaattgc gtcttcatgg cggcgcacat acggcccggc ttcccctaga
     2581 tcagcagcga cgagcgcagg acgatgaaca cggccaccag gacgaggatg aatacgatca
     2641 gcggcaggga tacgccgagg cccaccttga tccagtagcc gaaggagcgc tccacgtcga
     2701 cgaatccgcg gtgatcctcg aaggtcacct ggggatgttc cgccggcgcg cccttctctc
     2761 cctggatggc gtcgagccgc gacatggact tgttgtattc ctggcgggcg ttctcgagga
     2821 tgctcaacgc ccgcgtcagc tcgtcgcggt aggattcgtc gggccaggtc tcctcgttca
     2881 gttcctgcag ctcggccgcc atcatcttga agcgcttgcg gaccgcggcc aggagttcgg
     2941 acagcttttc ggcctgcagc tcttccttct ccagggtcac gaggctctgg ccgaagcgct
     3001 ccagcatgtc gcgcttgccg cgctcgtatt cctcggtctt gcggcgcagg tcgtccatgt
     3061 cgtgcttttc ccgctcgatc tcctcctgcc gctggcggag ccgctcgatg tcctgcgacg
     3121 cctgggcgct ccgctcgccc atgtcctgct tgaaccgggc catgcgcgtc aggttgaaat
     3181 cggtgatggg ccggacgggc acctcatccg atgccggctc gcccatcggg accgcgggct
     3241 cgtcgcccgg ccccagcttg atgcgcttgg cgctgtcgcg ctgctttata agatcgtcgt
     3301 cgaagaaatc ggtgtctgcc atgggggcct cggggggtgt gggtttccta tttcgggatg
     3361 accagcgtct ggccgacccg cacgctttcg gggcttttca gcgtgcttcg gttggcctcg
     3421 taaatgtccg accagcgtcc ggaatttccg tacattctct ccgcgatgcg ggacaggttg
     3481 tcgccgggct gcacgatgta ggtggacatc gggggctggg cgggcgcggg ctccggcggg
     3541 cgcggagcgc ctgcgacctg cgccgcggcg ggcgcggccg cggctgtcgc ggcggcggcg
     3601 gccgccggga ccgccttcgc cgcttcgccg agccgggcgc gcagggcctc gatctcctgg
     3661 cgcaggaggg cgatctcctc aatggccccc gagggctggg cgggcagcgt cgacgcgaag
     3721 cggacccggg catgccggat gagatcctcg accaccgggc gtttttcggt ttgcggccgc
     3781 agttcgaggt agcgctggta atggtagagg gcgcggatgt aatcctgctt ctgggaatcg
     3841 tacaggtggc cgacctcgag atgggcgcgg gccaggttgg gcttgcgctc gagggccttg
     3901 ttgaagagct cgatcgcgcc gtccacgtcc tgctggttct tccgctcgcg cgcgcgcttc
     3961 aacagcggat cccgctcctc catctggtcg aggctgccgg cgctgcgcga gcagccgccg
     4021 gcggccatga gcagcacgag gcccaacccg ccgatccacc gcattccttt tctcacggac
     4081 acccgcatcc cgtttctcca agaccgattc ccgcgcgact cttcgacgcc gggattctta
     4141 accgcccccg ccccatggtc aagcgcaatg ccgtactccg ccggtcaggg ccgctcgaag
     4201 acccgccgga acacgaagaa gccgcgctta tccggccaga cctcgtcgtt ggctcgaagc
     4261 cccaccgcct gcgattgcag gtccacgaaa ccgttcggct ccatccagat gaaaggctcc
     4321 tccggcgggc ccttggttcc cgtgccgccc acggccggcc agccggagtc gtcatagtcc
     4381 gtcgcgcccc acgattcggc gggacggaac ttgtatttcc agtccggcga cgtgaccacg
     4441 aggccttcgt gcgtccgcac ggccgcctgc agccagctgg gatacgggct ccagagcgtg
     4501 tgcagggcca gcgcatgccg gccgggcccc gcggtcgcgc ccttcacgac ggcgcgctcg
     4561 ggccggccgg cggtgacggc ctcctgcccg tccagcagcg cgcgcgccgg cgcggtgctg
     4621 gacaggctca ccaggacatg ggagggatcc tcgtggaacc agagcagcag tttcgcctgc
     4681 tccaccgcct cgggatccgt ttcctcggcg gcgaaccgtt cgtactcggg gcgcacgacg
     4741 tcgaatccct ccagccgctc ccggtaggcc agctcccgta gcctcaacag cgccgccttg
     4801 gggaacttcg aatgcatctg caggaacgcc tcgatatact cccgggcgcc gcggtagtcg
     4861 ccgaagcgct cgtagttgag caactcgttc atgacggtgg cctccgccaa cgggtcgcgc
     4921 ggcggcgtcc ggcccagggc cgttccaagc gccgagcccg ccgcggcggg cgccgcgagg
     4981 taataccacg ccacgctttc catggcgccg cggctcgcct ggtcgggccc gcgctcgaag
     5041 tccatgcgga aagatttccc gaacttcgtc gcgtccagca ggtggaggcg gtactgcacg
     5101 acgcggaaga aggacttgaa ggggacgcca tgcagggggc gggcgatcac gttttggtag
     5161 taccagccgc cgttgaagta gtcctccagc cccgtccccc gccacttcgg cgcggcggcc
     5221 ccgtcggcgt acatctgctc atcgccctcg agaatccacc agctccggtc ctcgctcatc
     5281 acggcgagga tgcagccgac gtacttaccc gcgccctcgg ccaggaggat gggatgcggc
     5341 ttccccacgt cctgcgccgt cgtcctggac cagtgcgcgt gcaggtatcc ccagcccgct
     5401 tcccacgccg ggagcgactg gacgtccgcg cgcaccgaga ccgtgacggg ctgccccgac
     5461 tggttttcga tccggacccg cgcgcgcttt tcgaagggca tcgggaaccg cgacacatac
     5521 gagtcgttgg tcagggtgaa gaacatcgag gggaaccgga cccgccgcca tacctgcccg
     5581 aagaagtcgc ccagcggcgt ctccacggaa ggcgcatcgc tgtcgttcca gcggacctga
     5641 agcgccagtt gacggcccag cttttcgcgg gcgaccgcgt cgccgatcgg gccgtagtcc
     5701 ggcgtgacgg tcaactgccg gatcatcgcc gggccggcga catcaaggcc gtccgcgccc
     5761 tcgccgggct gcagcgtgag cgtggattcc cgggcctcgc agccgtccgg cacgggcgcg
     5821 agcccgccgg ccacccacgc gtcgcgcacc cgcaggattt ccgcaacgac ctcgggggaa
     5881 taggttcccc tgaactcctc cgtggtcatt ccgggcggaa gccgcgagaa gttgatctgg
     5941 aagaacagcc gcggccagcc gccaggttta aagccgccct cctgcaccat gaccactagt
     6001 cgcttccgga acgggatcgg gaccaggttg aaccagcaat agttctcgta ggccgccagc
     6061 gggtggcgcc agggatcggc gccgccgcaa aaaccgtcga gcgtcgtgtc cacggacggc
     6121 ttgcgctcgc cgtcaaagta gagccggatg cggtgctggc cgttctcggc gccggtgaac
     6181 cagaaacgcg acatgtagcc ggggccctcc aggtccgcca gcacgaccca gccctcgggc
     6241 cccttgcgga ggggatggtt atagtcgtcg ttcttgccgc tcggatccac ggatgagagc
     6301 agcgtcgtgt ccgggacgtc caggcgggcc gcctgcaggg ggtcggccca gcggcggatc
     6361 aactcggccg tggacagcac ctgctccggc ccgctccgcg agcagccgcc cgcgcttagc
     6421 aggaggaggg agaaaagaac gcttcggagt ttcatgggac ggactgtagt ggaggccccg
     6481 cggcgagtca acgcccgggg ggcgattctt gacattcagg cggcggccga caagagtatg
     6541 aagagaaggc cgcgtcctgc ctggaggcag gcgcgccgcc tgcatcgcga gagagaccgg
     6601 cgccacatcc cgaaccgagg agcgaacatg agcggaaaac gcgaggacga atgctgcccg
     6661 gagttcgatc ccgggccgtg ggacgacaag gagttggagt ggcgggatcg gcttttcgtc
     6721 cgcgaccgcg tgagaagctt cctgcacatc ccgctgaatt tcggcgcggt gatgaagcgc
     6781 aacatgatcc gaatccaggc ggcggacgcc gagcccgccg cagcggccgt gttgtcggac
     6841 gagaattccc tgtggggcgc ggacgtctac atcgaggtca ccaaggagat cccgggcgcg
     6901 ccgatggccc ggatctcggg gacgtttctt tcccgggtct tcgagggacc ctaccggaac
     6961 atgggcgcgt ggatcaaggc gatgcagcgc cacgtgaagg agaagggccg gacgctccgg
     7021 aagctgtact tctattacac gacctgcccg aagtgcgcga agaagcacgg aaagaactac
     7081 gtcgtgctcc tggcccaggt ctagcccgcg cgccggccgc ggccgtaggt gctggcccac
     7141 gaggcggcgc cgggcttctt cctccacgca tcgaggcatc ggcgcagggg cgagggctcg
     7201 acggcgatgt catcgaggcg acgaatgggc caccagaaga attcgagctt ctcttcggtg
     7261 gaaggcacgg gcgcgcccgg gcggcgggag ggaaggcgca gctcgaagac caggttgatc
     7321 tcgcagacgc gctcgccctt gtggcggaag gtgtgctcga ccgcgccgag gaatcgcccg
     7381 acgcgacagg cgaggccggt ctcctccttc atctcgcggg ccagcgcaac gggtgcgctt
     7441 tcgttccatt cgatgtgacc gccgggcagg tagcagttcc ccttcttccg gttccggcag
     7501 accagcacct tcccgccggc caccagcaca ccccgcgcga tgaactcgat ttccttcatg
     7561 attcctcttt ctccgcctct cggcacagga cgtccagatc gtgaatgccc atcgtctcga
     7621 aaacttcgcc ggccagaaac aacgaaccgg cgacacagac ggccccgtcc ctttgcgcgg
     7681 cccactcgcg ggcctggcgt agcgccttgc gcaggggcag gccctccacc tccaggcctg
     7741 cggcccggat gccggccacc agcctgtcca gcggcagggt ccgctcgttg cggatctgca
     7801 ccgcccaggc tctcttcacc cgcggggtca actcgcgcat gaaaccggca aggtccttgt
     7861 ccgccaacag gcccaccacc agggccaatt cccggcggcc cagcacgcgc ttgagagtgt
     7921 cggccaggac ccgcgccgcg tccggattgt gggccccatc caaaatgacc gccggatccc
     7981 tggacaggac ctggaaccgc gcgggccacc agacccgctc gaggccgctc cggatggccg
     8041 cctcgtcgca ggagaacgag gacaggctgc ccaggtattc gagcgcggcg acggcggtgg
     8101 ccgcgttctc caactggtgg gcgccgggca gaggcaggat gagcgggccg tacacggccc
     8161 cggcggtttc gacctggatg ctctggccat ccagcgtggc gcggggcgac cgcacgttca
     8221 ccgcctcctc cgcggccacg cacggcgcgc ggcgctctcg cgcggtccgc tcgatgaccc
     8281 cgcgggcctc cgccggcatc gcgccgagca cgacgggaac cccgggcttg acgatgccgg
     8341 ccttctcgcc cgcgatctgc tcgagcgtgg tcccgaggta ttccgtgtgc tcgatcccga
     8401 tgcgcgtgat gacgctcaac agcggacgga cgatgttggt ggcatccagc cggccgccga
     8461 gcccggtctc cagcaccgcg atctggacgc cgctccggcg gaaatgctca aaggccatcg
     8521 ccgtggaaaa ttcgaaaaag gtggcctcgc gtccgccggc ggccacctgg tcgcgatcgg
     8581 cggcatccac acgccggatc aggccggcca ggtccgcgtc ggacacggcc gcgccgccca
     8641 cccggatgcg ctcgttgaaa cggacgaggt gcggcgacgt atagagcccg gtcacccagc
     8701 cagccgcccg cagggcgcgc tccatgatgg cgcagaccga tcccttgccg ttggtgcccg
     8761 ccacgtgcac gcagacgaac gcgcgctcgg gatgatccag ccgtgcggcc agcccctcca
     8821 tcgcctccag ccccatcttg atgccgtggg cggaccggcg gtacaggcgg tcgaggaacg
     8881 gcgcggctgg cgagtccttg gtcatggtcc gtgcggcgcc ctcccgctgc gcggcggggc
     8941 taggcctccg ccggggcggc tggtttcggc gcgggggccc cggccgggcg gcccatcatg
     9001 aagtcgagca gccgcacgat ggcggctttc aggtccttcc ggggcacgac catgtcgagc
     9061 aggccgtgct ggagcaggaa ctccgcgcgc tggaaacccg cggggagatc ctgctgggtg
     9121 gcctccttga tcacgcgggg gccggcgaac ccgatcagcg cgccgggttc ggcgacgatg
     9181 acgtcgccca gcgtggcgaa gctggccatg acgccggccg tggtgggatg ggtcaggagg
     9241 ctgatgtacg gcaggccggc ctcggcgtgg cgggccagcg cgccgctggt cttcgccatc
     9301 tgcatgaggc tcaacatgcc ttcgtacatg cgcgcgccgc cggaggcgca ggagatgaga
     9361 gccggaaggc gctcgcgcgt ggcgcgctcg atcagccggg tgaccatctc gccgacgacg
     9421 gagcccatgc tggcgccgag gaaattgaaa tccatcacgc cgatcgcggc ccggtgggcg
     9481 ccgatccgcg cgcggccgca caacatggcg tcgcgacagc ccgtcttctt ccggttctcc
     9541 tcgagcttct cgaggtacgg cgccacgccg gtgaatccga gcgtgtccac gctgtacagc
     9601 tgggcgtccc actcctcgaa actgccgggc tcgatcagca ggtccaggcg ctggcgccgg
     9661 ccgatctgga aatggtgccc gcatttcggg cacacattca gggccgcctc caactcctgc
     9721 ttgaaaatga tctcattgca ggagggacac ttcagccaca ggccgtccgg catatcgcgt
     9781 ttccggacct tgaccgtgga gtagttccgt ttccttaaaa aagccatggg cctctccggt
     9841 ccaccgtgtt acccgcgggc cagcgtggcg acgtagaccc gcgccgcgcc cccttctttc
     9901 aggacccggg cgcactcgtt taccgtcgcg cccgtggtca ttacgtcgtc caccagcagc
     9961 agccgctgcc cttcgatcca gaccggcttg cggacctcga aagcgccctt aacgttagcg
    10021 gccctctggg gggctgtcaa atgagtttgg gtcggggtgg gccggatccg ccgcaggacg
    10081 ccggacagga acggtttgcg caggcggcgc gcgagggcgg cggccaggag ggcggcctgg
    10141 ttgaatccgc gctcgcgctc gcgggcggca tacagcggca cggccgccac cgcgtcggcc
    10201 tcgaggtcgg tgaactgcga ctcgacgcag ccgtgaagga gacggtccag gtccgactgc
    10261 acccagaggg cggcgtcgta cttgaacgcg cgcaggacct gctgggccac gccccgatag
    10321 cggaccgccg accgggcccg ctcgaagtgg atctcgtcgg cggtgcacgt gtagcaggtg
    10381 taggcatggt ccacgcgccc ctcgatggga tccccgcacc gggcgcagaa aggaagttgc
    10441 acgaacggga tggcggagag gcaattccag cacaggaagc cctcggcatc cgccgtcggc
    10501 ccgccgcacc ccgcgcacca gcgggggtag gccatgtcca gccaccgcgc ggccgccgct
    10561 ttccatgaaa ggctcattgg gatcggctga ttacagcccg ccggggggat tccgcgcaag
    10621 gttctttttt tgcctttttt tccgtcccgg atcgggtaag ctacgcgcga tgaacacacg
    10681 ggaatttgcc ggtcattcca tcgtccgcaa cctggccggc ggggggatga cgcgcctgtt
    10741 cgtggcgctg gacagcgcgc agaaccgcgt ggtcctccgc tacctgctgg agaactgggc
    10801 gcgcaagtgg cggttccgca agcggttctt ccacggcgcc aaggtgctgg ccaagctgga
    10861 caacccccat atcgtcaaga ttctcaagac cggctacgag ggccgtatcc cctacatgac
    10921 gatggagttc gtcgagtccc ggacgctgcg cgacctgatc ctttaccgcg aaccgctgct
    10981 cacccagaac gtgctgtccc tgatgcgcca gatggcggcg gcgctcttct acatccatgc
    11041 ccagggctat ctccacctcg actggaagcc ggagaacatc ctggtccggc ccgacggcca
    11101 cattgtcatc atcgatttcg acctggccat gaagcgcaag cgccggccga tccgcgtcaa
    11161 ggagttgccg ggcacgccgt cctacatcgc gcccgaagcg ctcacccggc atctcgtgga
    11221 cgagcggtcg gacatctaca gcctgggcgt gaccttctac gagatgctga cctaccacaa
    11281 gcctttcgag ggcgacaaga tcgagcaggt gcgcgccgcg cagattgatc cgaacacgcc
    11341 ccccacccgg cctcgccagt acaacccgga cgtcccggtg gcgatggaat cgctggtgct
    11401 caagtgcctg gccaagcaac cggatgaccg ctacccgtcg ttgagcctgg tcatccgcga
    11461 cctcgaggcc ctcctatgaa ggacctgtac ctccggcccc gccgctcgac gcggggctgc
    11521 ctgatcgcgg tgattctcct gctgctggtc atcctggcgg cctggtggtt ctggcggcgg
    11581 ccgaaggcgg aagagccggc cccggattcc ccggcggcga tcccggcgac ggccgccgcg
    11641 cccgcccccg tccccgccgc gaaggaaccg cctcccgccc ccgaggcaaa agcgcccgct
    11701 ccgaaggcgc ccgtcgcctc gcggccttcg ccggccgcgg ccgaccccgg gctggacctg
    11761 ttggcgcgcg cccgcgagtt gcgcgcgtcg gacaagctgg tggaggcgcg ggacgcggcc
    11821 tggcaggcgc tggcccaatc ctccaacgag gtcgcccgga cgggcgccga ggcgctcctg
    11881 aacgaggtca acatcgaact gctgttgagc ccgcggatga tgccggagaa ggaggagtac
    11941 gtggtcgcct ccggcgattc gctggacaag ctccgcaaga aattcgggac caccgtcgag
    12001 gtgatccggc gcagcaaccg catcccgggc gacgtgatcc gcgcgggcga ccgcttccgc
    12061 atcttcaagg gccggttcac gatccaggtc agcaagagcc ggaacgacct ggtcctgatg
    12121 atgaatgacc ggtttttcaa gcggtaccgc gtgggcacgg gcgaatacgg gaagaccccg
    12181 acgggcgact tcacgatcaa ggaaaagatc gcccagccga cctggtggcg gcccgacggc
    12241 aagcccatcc cgttcggcga tcccgaaaac gtgctgggca cgcactggct ctccctcgac
    12301 atcccgggct acgggatcca cggcacgtgg gagcccgaca cgatcggcaa gcaggccagc
    12361 gccggctgca tccgcctgct caacgcggaa gtggaggagc tgttcaacct ggtgccggaa
    12421 ggcaccccgg tccgcatcgt ggaataaaac ctggcggagg aacatccgtg cccccttaca
    12481 tcctgccctt cgagaaaccg atcctcgaca tggagaagaa gctggaggac ctgcgcgcct
    12541 tcagccaggc ccagaacatc gacgtgtcgt tcgagatcga gcggatgcag aacaagatca
    12601 cggagatgcg gcgcgagatc tacgagaacc tcggcgcctg ggacaaggtg ctcgtggcgc
    12661 ggcatccgca gcggccgtac atgctggatt acctggccgc gatgacgacc gatttcacgg
    12721 agttgcacgg cgaccgcgtg cacgccgatg accgggccat tatcggcggt ttcggctggc
    12781 tggaggggcg gcgggtgatg ttcatcggga cccagaaggg ccgggacacc aagagcaacc
    12841 tcgagcgcaa cttcggctgc gcctaccccg agggctaccg caaggcgctc cgcctgatga
    12901 agctcgcggc gaagttcggg gcgcccatcg tgacgctgat cgacacgccg ggcgcgtacc
    12961 cgggcatcga gtccgaggag cgccacatcg ccgaggccat cgcggtgaac ctgcgggaca
    13021 tgttccggct gcccgtgccc atcgtggtcg tggtgatcgg cgagggcggc agcggcggcg
    13081 cgctgggcat cggcgtgggc gaccgcgtgc tgatcctgga gtacagctac tactcggtca
    13141 tctcgccgga gggctgcgcg gcgatcctgt ggaagaaccg ctcctacgcg aaccgggcgg
    13201 ccgaggccct caagctcacc gcgccggacc tgctcaaggc cgggctggtc gacgaggtgc
    13261 tgccggagcc gccgggcggg gcgcacgcgg accccgcggc catgtccgcc acgctgaagg
    13321 cggcccttct ccggaacctg gaggaactgg acgcgacgcc cgtggagcag cttctcgagc
    13381 agcgttacaa caagttccgc gccatgggcg tgttcgggga ggacgccgcc ccggcgccgg
    13441 ccgcgagccc cgcgccggcc tcctgattca gtaatacaac acggatccct ccggatcgta
    13501 gcggacgtac atgcgcgctg tgtacccgaa tcccgcgcag gacccggtgg tccgcgacat
    13561 ctcgaggcga tagtaccccg gcggcagcca gagggacagg gcctcgacgg cggattgccc
    13621 gccgccctgg ttcacgccgg gatggaacga ttccgcgacc tgttgctgcc ggccgtcggc
    13681 gcccagcgcg tacgcgcgca agcggacgtc cacgcccggg ctcccacccg ccacgaccca
    13741 ctgcgcggcg gcatggacca tcgcgtggtt cggcaggtgg aaccaacggc cgaccgcggt
    13801 cacgggagac actgcttcga gggtcatcgt gttcgtgtac acgcaccggt catcgcccat
    13861 gctcatcggc aggttgaaat actggtaccc cgtgcgccag gcggtcaggt tcccgggcac
    13921 gtggcgcgtc tcccggatgc agagggtgct cgcccgtccg ccgtgctgcg acagccactg
    13981 ggccatgtcc agctgcacgc cgaaatagat gctgttccga tcgcccgcca cggcataccc
    14041 gtccatttcg ggcaactgga tgtccaggag aatgnnnnnn nnnncccgcc acggcatacc
    14101 cggccgaact gcccaggaaa atcccgtcca ggaagaagtc caccttatac ctcgcgccat
    14161 cctgcgcgcc ccagttgaag ggcttcttca cggcgagcgg gatctcgcaa gcattatatc
    14221 ggtaggtttt cagcggctgc gccggaagcg gtagaggcga catcacttgg aagcccagcg
    14281 ctccggcgcc cccctcgctc accaggatgg aacggtaata cttgtagccc gccacgcgga
    14341 accggtcgcc ggcgcccgca aacagggtcc cgcccccgcc gttggcattg ttgagatacc
    14401 gcaccttgat caccccgccg gtaacggaaa cggtatcgaa cgccccggcg ctcccggtaa
    14461 ggatctggac cacccggtcc cgcccgtcca ggatctcgat gcgggagttc tcgcccggat
    14521 agccggcgct cttgatcatc aggcccgtgg attcaaaacg gaccttcatg gacgtcgcgc
    14581 ccggatgccg gaacgtccag gtcctggttt cgtagggcgc gtactggaca tactccatgg
    14641 gcgcctccac gaggcaccac tggtaccggg gcaacgcacc gggcagatcc ggtttcggcg
    14701 gaatctgccc gcgcgcgacc accgccatgc cggccagcat ccacaacgct attttcaaga
    14761 acgcttcgat cctcttcatc gtcgtatgct ccttttttcc cggcagcctg ccggtatcgc
    14821 gaccctgctc cctgcgagcc tccatgacat cctgcacgga ccgagccgcg ccggccgctg
    14881 gctttgggac caagccgcct ccggcacgcc cgatgatagg aaagtcgtcg ccgaatcgtt
    14941 tttcgtcctc ttttgtaata attatcttgc cagacatgac atcggcccta tttttgtgct
    15001 acatttttaa gacggattaa aaatcgttta aggctcttca tggggtaaat acgtgccact
    15061 gttgtccaga aagccgcgcc ctataaccat tcatgatgtg gcccagcgcg ctggcgtgtc
    15121 gatcgcgacg gtttccaacg tgctcaaggg gcgcatgtcc gaggtgtccg ccgccaccgc
    15181 ggaacgggtg cagcgggtcg tcgagaagat ggggtacgtc aagaacctga cggcggccgc
    15241 cttgtccagc cagcggtccg gcctggtcgg cgcggtcttg agcaatatct tcagcgcctc
    15301 gcccagccag ccggacgtgg agatcaaccc gttctacggg gagttcctgc tcctcctcga
    15361 ccgggaactc cagccgacgg gcagcacctt gtgcctctac accggccggg aagaaacctt
    15421 cgtcaacttc ctcctcgaac ggaacctcga cggcgccatc ctggtgggga tcgtggccaa
    15481 ggaactgccg cccgtcctcg agcggaggga aagccgcctg gtgttcttcg attccttcct
    15541 gcagcaccgc gtctacatgc gcgtccagac ggacgagctc aagggcggcg gcatggccgc
    15601 cgattacctg atcgcccagg gccgcaagca cctggtattc gtgggcgggg ccgtccgcga
    15661 cctgccgtcc aacatccccg ccgtccgttt cgagggcgcc agccgcgtct gcgaccagtc
    15721 cgggcttcgg ctcatgctga tcgaggaatc cacttctttc gaaggcggcc ggcgcgcggc
    15781 gaggcgcgtg ctcgacctga aggccgacgg cgtggtggcc gcggcggacg tcatggccgc
    15841 ggggctgatc cgggggctgc aggaggccgg cgccaaggtc cccgatgatg tcgccgtggt
    15901 gggctacgac gatctccctc tcgcccgcat gatctccccg cccctcacga cggttcgcca
    15961 gggcctgccc gagaaagtcc aggccgccgt ggaactcctg aaagacgggg aaaaaggtga
    16021 ggtgcggatc atcaagccgc acctcgtcgt ccgggaatca gcctgacgcc gggccggtcg
    16081 ccggattctc aacgggcaga tccagccgga tgtgcacgtc ccggagctgc ttcgcgtcca
    16141 ccctggccgg cgcgttcatc atcaggtcca tcgccttctg ggtcttcggg aacgcgatga
    16201 cgtcgcggat ggattcgccg ccgaccatca gcatgatcag ccggtccagc cccagcgcga
    16261 tgccgccgtg cggcggcgcg ccgtagccga aggcgcggat caggtgcccg aaccggtcct
    16321 cgatctcggc cgtcggaatg cccaggatct tgaacatccg ggcctggagt tccgggtcgt
    16381 ggatacggat gctccccccg cccagttcca cgccgttgag cacgatgtcg taggcctgcg
    16441 cccggacccg gagcgggtcg ctgtccagga aggccagatc ctccgcgtgc ggcgaggtga
    16501 acgggtggtg catggcctgc agttcgccct cgtcgttcct ctcaaaaaga gggaagtcgg
    16561 tgacccaggt gaaggcgaac ttcttcggat cgatcaggcc ggccgcctcg cccgcgatca
    16621 gccggatgcg cccgagcgcc gtgttgaccg tcgcgagctt gtccgcgccg aacatcacga
    16681 ggtcgccggg ctgcatgccg agcttcttcg tcaggccctg ccgctcgccc tccgagagga
    16741 acttcgcgat cggcgacttc cactcgccct cgggcgacac gcggatgtag gcgaggccgc
    16801 cgagcccggc gtccttggcc atcgcggtcc aatcgtccat cacggacagt gaggtgttgc
    16861 cctgcccctt cgcgttgagg gccttgacca cgccgccctt cgccagcacg ccggcgaaga
    16921 ccttgagctg ggtgcccgcg aagacgtccg agacgtccgt catctccatg ccgaaccgga
    16981 cgtcgggctt gtcgctgccg aagcggttca tcgcgtcgtc gtacttcata cgcggcagcg
    17041 gcagcgcgat ctcgccgtgg cccgcggcct tcatgatgat cgccagcagg ccgtcgatga
    17101 tcccgtagat gtcctccggc gtgacgaacg aaagctccat gtcgatctgc gtgaactccg
    17161 gctgccggtc ggcgcgcagg tcctcgtcgc ggaagcagcg ggcgatctgg tagtagcggt
    17221 cgaacccggc cagcatgagc agctgcttgt actgctgcgg cgactggggc agcgcgtaga
    17281 acgtgcccgg caggatgcgg ctgggcacga ggtagtcgcg cgcgccctcc ggggtgctct
    17341 tggtcatcat cggcgtctcg atgtccgtga agccggcgcg gtcaaggtag acgcggatcg
    17401 cctgcaccat ctcgtgccgg tagcgcatgt tcttctgcat ctgcgggcgg cgcaggtcga
    17461 ggtagcggta ggccatgcgc aggtcctcgg aaaccttcgc ggcctcctcg tcgtccagcg
    17521 ggaacggcgg cgtcgcgctg cggttgagga cggtgatcgt gttggatcgc acctcgatct
    17581 cgcccgtcgc cagcttcggg ttggccatgt ccgcgggacg cggctcgacc gtgccctcga
    17641 tccggatgac gtactcgccg cgcgagccct gggcccggtc ccaggcctcc tgcgaatcgc
    17701 gcgggtcgaa gatgacctgg gtcaccccgt accggtcgcg caggtcgatg aagatcacgc
    17761 cgccgtgatc ccgcaccgtg tccacccagc cgcagagctg gacgcgctgg ccgacatggc
    17821 tttttctcaa ctcgccgcag gtgtgcgaac gcatcatggg ttcaagttcc ttccgcccgg
    17881 gacgcccgga gcagccgttc caccagggcc ggcaattcca gttcctcctg cgccccgtcc
    17941 ttcatgcttt tcaaaacgaa caggcccttc tccatctcca gttcgccccg gatcacggca
    18001 aaggccgcgc cgctccggtt ggccgcccgc atctgggcct tgacgctccg cccgtccagg
    18061 gcgagcccgc aggcgatgcc ccgctggcgc agcagctgcg ccagcaccag gttctgttcc
    18121 agggccttcg cgccctgcga cacgatccac gtcgcgggct ttccgcgcgc cgactccgcg
    18181 gaggccggcc ggtcgtgctg cacggccgtt acgatgcgtt ccaatcctgc cgcaaacccg
    18241 acgccctcga tcgcggcgcc ctcccactcg atgcggtatc ggcccccgcc cgccagcgcg
    18301 tcctgcgcgc ccagggccgg gtgccggatt tcccagatcg tgtgctggta ataatccagg
    18361 ccgcgcacga gccggggatt gatcacggcc ggcaactcga gcaggtccag cagccgccgg
    18421 acttcgtcga ggtaggcccg ggaatccgcg cccatgaaat ccgtgacggc cggcagcgtt
    18481 tcggccaccg cgcggcagcc ctcctgcttg cagtccagca cccgcagcac gttggccttc
    18541 agcctccggc ggcagtcgtc gcacaacctc ggctcgtgcg gctcgagggc cttgcgaagg
    18601 ccctcgctca ccgccgcctg ctccgcggga aggccgcggc tgttgatctc gatccgcgcg
    18661 gccttcagtc cccacgcgtt caacaggtgt tgctgcaggg cgatggcctc gacatcctgc
    18721 gcggggcccg gcggcccgat cagctcgacg cccagctggt ggaactggcg gcgacgcccc
    18781 gcctgcgggc gctcggcgcg gaacatcggg cccaggtaat acaggcgggc cccggcggcc
    18841 tcgggcccga gcccggccag gtagcggatg accccggccg ttccttccgg ccgaaggctc
    18901 accgaccggc cgccgcggtc ctcgaaggtg tacatctcct tctgcacgac gtccgtgcct
    18961 tcgcccagcg agcggacgaa gagtcccgtg ggttcgagga tcggcgtgcg gacttcttcc
    19021 acctggtaca gcgccaggag gcggcgggcc gcctcctcta cttcctgcca gagatggact
    19081 tcgggggaag caaggtcaga catgccttgc aggggctgaa acgacgggac cgtcatgagc
    19141 tttcaggcgc ggccttcaag ccccggtgat caactgcttc atgatgcgac ccggcttgaa
    19201 cttgaccacc ttgcgggccg ggatcgtcac cacctgggtc gggcgattgg ggttgcggcc
    19261 gatgcgcgac ttgcgctcgc gaacctcaaa caccccgaag ttgcggaact cgatcgtgtc
    19321 acccttcttc agggcctcga tgatgtagtc gagggtcttc tggatcacgg caaacacatc
    19381 ctgctggatg agccccgtct cgtcggcaat ccgcaccacc agctctcgct ttgtcatagt
    19441 tgctgcgctc cttcgccccg ccgggttgtc cggcccggcc gggcgtttca cccggctatc
    19501 tgcttcccaa gcataggata ataaacgcgg gaccccgcag gtcaagggta aagtcggtcc
    19561 cgaaaacgcc tcaccggacc acggtatgaa tgtagccgca gatcacggag cccacgagca
    19621 gcaggagggc caggcttttg ttgaactgca tgaaactggc cagttcgtgc gcggtcttct
    19681 cggcccgaac ggcttccgac gggaaccagc cgtcccggac gcaccagagg cccaacagca
    19741 tcagcacgat cgcggcgatc aggaaatgct tcgagccttg aacatgatat cgacgggcca
    19801 tgcctattcc ttcctgccgc gcttcattcc ccgccgggcg cgcacgcgcg cttgagttcc
    19861 gccagccgcg cgcacgcggt ttccacggcc gattccacgg gcaccaccag ggtctccccg
    19921 ctgtgccgcg cgcgcagctc gatcgagccc ttcgcgaggc cccgctcgcc gacgttgatc
    19981 cgcagcggca gccccagcag gtcggcgtcc ttgaacttca cgcccgggcg ctccgcccgt
    20041 tcatcaaaca atacctcgat gccgacattc ttcaagccct ccacgagctt cccggccgtc
    20101 tccacgctcg ccgcatgctg ctcgttaatc agcagcacct cgacctcggc cggcgcaatc
    20161 gtcgcgggcc agatgatccc attgtcgtcg tggctctgct cgatcaccgc ctgcagggtg
    20221 cgcgtgacgc cgatgccgta gcagcccatg acgcacggcc gcttctggcc atcctcgccc
    20281 aggtacaggg cgcccagctg ctcgctgtac ttggtgccga gcttgaacac gtgcccgacc
    20341 tcgacgccgc gcttggcccg cagcgttccc gcgcagcgcg ggcacccgtc gccctcgatc
    20401 gcgaagctga tgtcggcgaa ctccgtcacc ttcacgtcgc gctcgatatc cacgctccgc
    20461 aggtgcgcgt ccgcctcgtt cgccccggtg acggcgccgc ggtagccccg gagcttggcg
    20521 tcgaccacga gcgggatgga caggccgacc ggcccggcga accccacggg cgcgccggtg
    20581 acccgcgcga tcgtcgccgc gtcggccagc tccagcgcct tcgcgccgag cgcccgccgg
    20641 agcttgatct cgttgacgtc gcggtttccg ggcacgaggg ccgccaccgg cttgccgtcg
    20701 gccacgtaaa tcagcgtctt gatcaggcgc gcgggcggcg actggaagaa gccggccacc
    20761 tcctcgaccg tgcgcaggcc cggcgtcgcg atcttttccg ggtccttttc cgcgtcggcg
    20821 aacgacggcg ccgagcggag cacgctctcc gcccgctcca ggttggccgc gtaggagcag
    20881 gcgtcgcact cgaccaggcc gtcctccccc gagtcggcca ggaccatgaa ctcgtgcgac
    20941 gagcttccgc ccatcgcccc cgtgtccgcc tcgacgatct tggcgcgcag cccgcaccgc
    21001 tggaagacgc gctggtacgc ctcgtacatg gaccggtagc ttttctcggc gccgtccagg
    21061 tccgcgtcga agctgtaccc gtccttcatg atgaattcct tggcccgcat cagcccgaag
    21121 cgcggccgga tctcgtcccg gaacttggtc tggatctggt agagattgac gggcagttgc
    21181 cggtaggaac tgacctcgtg ggcgaccagg tcggtcacga tctcctcgtg cgtgggtccg
    21241 aggaccatct cgcgctgctg gcggtccttg accttgaaca tcacgtcgcg gagcagttcg
    21301 taacggccgg tctgctccca gacctccttt ggatggagcg cgggcatcag gatttccagg
    21361 gcgccggcgc ggttcatctc ctcgcggacg atgcgctcca ccttgcgcag cgcgcgcagg
    21421 ccgagcggga ggaaggtgta caggcccgcg cccagcttgc ggatcagccc ggcgcgcagc
    21481 atgagttgat ggctcgggat ctccgcttcc tgcggcactt ccctcaatgt cggaatcaat
    21541 tgtctggacc agcgcacgat atccatcctt cggtaggggt ttgcggcttc agccggacgc
    21601 ccgttcgcgc ggtgttttcg ccgttttgaa gccccagcgc aacaaaaaac cgcgccggcg
    21661 cggcccgccg ctcttttcca ttgcgccggt tccgcggggc gggtattttc gcgccccgag
    21721 gaacatcacc atgaaaaaga agatctacct ctgcgggccg atcatggacg agcacgaggg
    21781 cctggcgcgc gcctggcgcg aggaggccaa gaaacgattc ggccaggatt tcatcctgct
    21841 cgatcccatg cggcggaact tcaaggaccg cgaagtggac agcgccaacg agatcgtcga
    21901 gttcgacctg caggacatcc gggacgcgga catcctgctg gtcaattaca gcaagaactc
    21961 ggtcgggacg tccatggagg tcttttttgc ctcgcagaac ctgggcaagt tcgtcgtggc
    22021 cttctcgccc tacaccttca aggactgcag cccgtggatg gtgcggttct gcacgaagat
    22081 cctgccgaac ctggaaaccg cgacggacta catccgcgag cacttcctgg tgcagcacat
    22141 ggcgtgagcc ggggcggccc cgcgcgaaag gtcaggtgcc cttggtcttg ggcaggccgt
    22201 aggcgcgatc gccgtctttc cacttcccgc ccttcttgag cgattcgatg cgctcgaacc
    22261 gcttcaggac gttccgtttc gccgcgatct tgctgccggt cttccaactg gagtgaagag
    22321 acatgccgcc atcctacttt tgtcggtccc cgcggacccg ttttgttttt agcagggcgt
    22381 acagataaca gaaagcgcgc ggatgtcaat ctccggcatg gccggagacc gggtccatcc
    22441 acaggaggcg cgggagttcc gcccagcccc gctgccggcg gaggcgcgcc aggaccgcct
    22501 cgccgtgccc ggccaggtcc gcccgccaac gccggagcgc gcggccgctg atccccgcgg
    22561 gaacgccgcc gaccacgccc agcacgccgt ggcccccggt cgcatcccac aattcggcca
    22621 tgtcctcgat cagccgtcga acgacgggga accacaacag ctccagccgg tagcacggca
    22681 gtccgcccac cttgcccagg tccaggatcc acacgcggtc tccgcccgcc cattccgccg
    22741 aacgatacag gcgcgaggcc aggccccgcc aggcctcgag cgatcgggcc ggggacccgg
    22801 ggccacccgg attctccacg ccgcgccacg cggcggcgat caggtgctcg acgtaatccg
    22861 aggagagcca ctgccgatcg tagcgctccc tccagaaggc cgcaacgccg cgcgccacgt
    22921 cgtccgacgc ttcgtccgcg tccggcgcga cgcgggccac ctcggcgcga agttcgccga
    22981 ccagggattc ttctccattc gtggccgagg tccagatcat tgccgcccga tcaatccttc
    23041 cacttcgctc aagaactggt tcacatcgcg gaaacgccgg tacaccgagg cgaaccggac
    23101 ataggccacc tcgtcgagtt tctccaggcg gtccatcacc ttcttgccga tgaccgtgct
    23161 cggcacctcc cgctcatact cgctctccaa ctcccccgcg attccgtcca ccagcgtttc
    23221 gatctgctgg gtgctgatgg gccgcttctc gcaggcccgc gtcaccccgt tgaccagttt
    23281 ccggcggtcg aaatcctcgt ggcgcccgtc gcgcttgatg acccggagat gggccttgat
    23341 cacctcttcg tacgtggtga accggtggct gcagccgaga cacaccctgc ggcgccgtat
    23401 gacatcacca ttccgcacgg aacggctgtc gatgaccttg tcttcctgct ggccgcactt
    23461 gggacatctc atggctgcac acccgttcga taacccctct ccattgggtt aggttaaaga
    23521 atgggacacc atatgtggga ctgtcaaggg aaacttcgcc catcttggaa agaaaatggc
    23581 ggtcctgcca tttttccggt gacaggaacc gccatttcag atacgaattc tgttggattt
    23641 ccggcttggt gaaaagccgg ttccatgaag aagaatttac ttcttcgccg ctttcttctt
    23701 ggcgggtttc ttcgccgctt tcttcttggc tgccattgct atctcccccc cttcatcccc
    23761 gggcgttgcc gtcccgggat tggttgaccg ccggcgctgc ctacgcgatt cctcttacgc
    23821 gggtgaaccc gcctcgaagg gcatctatct tcagcgccaa cctctcctcg gcccctcctc
    23881 cgcataaggg cgccgactca agacaagacc tgccgttcgt tctcccgatc ctgcgcgacg
    23941 gagatcggag tgccgccgat ctctcgccga cttcatctaa aaaagaacag gatgccgtca
    24001 acgcgttgca tcgattggaa aaataaatcg cctccgccgt gtcgtcaacg cttatcgtga
    24061 acttttttta agcgtacgag catctgctgc tcgatccagc gcacgaggcg aacccccacg
    24121 cgcgccttgg tcatcagcgg ccacgcctcg cgcgcgccgt ccgccgagag aaggatcacg
    24181 cggttggtgt cggtctcgaa ccccgagtcc tgccgcgaca cattgttggc cacgatgaga
    24241 tcgagcccct tttccttccg tttcctccgc gcgtctttct ccggcgctcc ggtctcggcg
    24301 gcaaagccga cgtatatccg ccggcccttg agcgcgcgca cgtcccgtaa gatatccacc
    24361 gtgggcacga gtttcagcga gagcgcgcga cggttccgct tcttgatttt ttttacggcc
    24421 gttgcggccg gacgccagtc cgcgaccgcc gctgacatga ccaatgcgtc gcaccagcgg
    24481 atgtggcgtc gcacggccag gcgcatctga tctgccgtga cgacattcac aacattcata
    24541 ttcgcagccg gttgcaatga tacggggccg gagaccaggg tgacctcgtg accgcgatgg
    24601 gccgctgcgg cagcgatcgc ataccccatt ttcccggacg agcggttgct gatgaagcgg
    24661 acaggatcaa tggcctcgcg ggtcggaccg gctgtcacca ggatcttcat gcgcccagcg
    24721 cgcgagagac ttccgcgacg atcgcgtcga cgtctgcgag ccggcccgca cccacgacgc
    24781 cgcacgcgag ttcgccgtgc ccaacgtcca tgaccttcac gccccgctgc cggagcgtgg
    24841 ccaggttggc ctgtgtcgcc ggatgcttcc acatcttttc gttcatcgcc ggggccacga
    24901 cgagcggcgc cgtgcaggcg agcgcggtcg cggtgacgat atcgtccgcc aggccgcagg
    24961 ccagcttcgc gatcacgttg gcggtgcagg gcgcgatggc gagcgcgtcg cacgacgcca
    25021 ggtccagatg ctcgaagacc gacgccggcg tcgcctcgca tttccccgcc gcgacgggat
    25081 gcccggtgag cgcgtggaag gtcagcggcc cgacgtattt cgtcgccgcc tcggtcatca
    25141 tgacccagac gtcccacgac ttcgccgtaa accggcgaac cagttccgcc gccttgtacg
    25201 cggcgatgga cccggtgacc cccagcacga cgcgcttgcc catggccccc tactttcccc
    25261 cgcggatcag gtgatgctcg gccaggatga tcgcgcgcag gttgtcatag gcctccgcga
    25321 ggtcatcgtt gaccaccatg tacttgtact ccgtccaccg tgcgatctcg ttctcggcct
    25381 gcttcagccg gcgtgcgatg gtctcgtccg tgtccttgcc gcgcttggtg agccggttcc
    25441 ggagcacctc cagggacggc ggcacgacaa agatatccac cacctcgatg cgcatcgggt
    25501 ccccctccgg cgcccgccgc gcgtattccc ggacggcggc cgcgccctgg acgtccaggt
    25561 tcatcaggac gtcccggccc tcgcccaacg ccttgacgac aggggtctta ggcgatccgt
    25621 acaggttgcc gtgcacgatg gcgtgctcca gaaattcgcc ctccgccagc ttccgctgga
    25681 actgttcctg ggtcaggaaa tagtagttct ttccgtccac ctcgccctcc cgcggggcgc
    25741 gggtggtgct ggtgatggtg tagaagaagg acgggaactc ggcgtagagc ttgtcgcaga
    25801 gcgtcgtttt cccggcgccc gaaggcgcgg acacgataaa gacctgggga tgtcggggca
    25861 gcgtgatcat tctatgtttt gcacctgttc tcgtaggcgt tccagttcgg ctttcaaatt
    25921 caccacgcgc cgcgcgatcg cggcatcgtt ggccttggac ccgatggtcg cggcctcgcg
    25981 cagcatctcc tgggccagga aatccagcga tcggccggcc gcgcccccgt ccctaagcat
    26041 ccggcgggcc tgcccgatgt ggctcgccag gcgcgtcaac tcctcggcga tgtccgcccg
    26101 gtccgcgaag agggccagtt cgcgaagcac gcgctcgtcg gccgcttcct tgtcgaaccc
    26161 ggcgtcgcgc agccgcgccg ccagcttggc gcggtactgc ttcaccacct ctggcgcccg
    26221 ggcccggacg gcggacaggt cggcctccat gctcttcagg cgcttctcga tgtcgcgccg
    26281 gagaacagcc ccttcccgtc cgcgcatccg cacgagtccc cccagcgcaa gccggagcgc
    26341 ctcgcggatc accggccagg cctggtccgc ctcgcgcgcc gcgggctcga atcgcaccac
    26401 ctccggaagc tggagcagca cgtccggact gaaatcgccg cgcaggccga tgcggcgcgc
    26461 cgtcttccgc agggcatgaa cgcaggcctc ggccagatcc tcgtccacgc gcaggctctt
    26521 gcgacgccac gcggcggacc actgcacgcc gaccgtcacg ccgacgcgcc cgcgggacag
    26581 aacaccccgg atatcctcct ccacacgcgg cgcgagcgcg cccaggacgg gcggcaggct
    26641 gacggagcaa tccagctgct tgcggttgac ggagttgacc tccacctccg cgctgatccc
    26701 tccgcgggcc gcctggcccc gcccatgccc tgtcatgctc tggatgctca tgcctttggc
    26761 ccgccgtgct tggcgctcca cgcggacacc tgctgcacgt ctttatcgcc gcggccggac
    26821 aggttgacaa ggatcaagtc cttcttcccc cagcgtcgcg cgtttttcat cagccacgcc
    26881 acggcgtgga ccgactccag cgctcccagg atgccctccc accgcgccag ggtgtagaac
    26941 gcgtccaccg cctcgcggtc cgtcacgtgg ctgtagtccg cccgcttcaa atcccgcagg
    27001 agagcgtgct ccggaccaac ggcggcgtag tccaggccgg cgctgaccga gtgggtcagg
    27061 cgaatctgcc cctcgctgtc ctgcagcacc cacgtgcgcg taccctgcag cacccccacc
    27121 c
//
LOCUS       DAOOTK010000032        27106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1258_length_27106_cov_4.934309, whole genome shotgun sequence.
ACCESSION   DAOOTK010000032 DAOOTK010000000
VERSION     DAOOTK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27106
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1258_length_27106_cov_4.934309"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..916)
                     /gene="folD"
                     /locus_tag="PK634_05670"
     CDS             complement(26..916)
                     /gene="folD"
                     /locus_tag="PK634_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOW97258.1"
                     /translation="MTAKILDGKALSRQLRAELAAQVAALRAGGIVPGLGVLLVGDNP
                     ASRAYVTAKEKACGELGMHSSEVRLPASASRGEILRVVSEFNRDPRVHGILVQLPLPD
                     GSMEQEVLETVDPAKDVDGFHPVNVGRAVLGLPAFLPCTPHGILQMLRRAGIPVAGAH
                     AVVIGRSHIVGRPLANLLSQKGEAGNATVTLCHTATRDLAQHTRHADIVIAAAGRPNT
                     VRADMIREGAVVVDVGMNRVADSSAPRGYRLVGDVAFDEVREKASWITPVPGGVGPMT
                     ITMLLFNTVAAARGGPAGSL"
     gene            1042..2151
                     /locus_tag="PK634_05675"
     CDS             1042..2151
                     /locus_tag="PK634_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97259.1"
                     /translation="MPTLSRMQERIAQRPSLVDALRQIAAGLLAAATGILLLLFLVSS
                     LYGGFKMATPLGSLSCRHPGPLLLWTALCAGVWALMSRPATRGEDDAAPPFSRAGAIG
                     LLLAATALLWLRFLYRSYEPVFGHRPVPTVPFLFTHLLFLFSGLAALGVWRQLRRTEA
                     EPAALFAALFTALSPLWFHVSAPVVAGYAAIALLWWGAPRLERAPGWLLATALIPVAA
                     AVLRHWGWAGNRLTDQFAAAAPGLCAWLLLPGLAGWWFSRRTRRPWMRAALLACAWSV
                     VCAGARGEAGSLGAVLGLPLIASLLAVGGGWLLSAVPAERGWLARNVIMICLAGILLG
                     MVRDGLDRSIRSPFAPPLPGPEAPAAASEPEEAPE"
     gene            2148..4073
                     /locus_tag="PK634_05680"
     CDS             2148..4073
                     /locus_tag="PK634_05680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW97260.1"
                     /translation="MKPAGDRRSLAIAAALLGLCLLAFFFKYELRYGPAPYLFYGDAN
                     TGQPNSDAVTWNIHALHLVEGRGFGDYLKQFRSRNYVPPGHPLILGLFYYLFGKDPEA
                     AGWCVAILFSLLPWVTYLWGREMWGRRVGLIMAALAALHAPYIHIGFSLMSEPTTVFT
                     TAVSLWFGARMIRRPGLSSAALAGLAFGFSALVRPSALAFLWGLAVALLMARPPAGGE
                     AVRTPGRPRLVALAALILCTLVPLGLWQYRNYRVHGRAAAVYSSISARHAWHGAHPKY
                     GPGFYSREAWHETLWREPYAPELAMIQRLQAETDEFIRADRFKYWMSCIWRIKVLFPE
                     FMHREAGIRWSHGGIGRIHFMALSAMAILGLLRALPLRVRLSGPDGSTDLPGALWAAS
                     LLIGLAAALAGAGIYGASDRYRWPLEFALIPFAALFLDELLRLSRAPFFTRGALTSSW
                     PPLPAPLRFARRLGLAALALVVLAYAAGLARARRHPDSPEDAAPRLTEAQVLRGIDRV
                     GLRTEFDGQSPRWITYDQVFTEQAANFGALTGLNMKLVAWWGRLRFVQTDSRGLARAG
                     YVILDPRPGDFGGARLPIRRAPNARGPYRHVRDGDAVTLLARLYYEPGPIQTPLLVFY
                     DILPGRFDGPAPGGAEP"
     gene            4070..5128
                     /locus_tag="PK634_05685"
     CDS             4070..5128
                     /locus_tag="PK634_05685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW97261.1"
                     /translation="MTDSRAAWWWKRLLLAMVTAGATVVAGELALRLVRFSAPVQPIT
                     IVDREGKSDPGDNRGLIPDSELQFRFNPGAEWRGRRINSMGFLGREVESNKAPGTVRV
                     ICMGDSCTATGNPPYSDFLHELLTGSPPTPQPWEAFNMAVHGYSTEHGLRLFRRRTRH
                     LQPDYVTLYYGWNDHWRDDLTDRLKLARRAGPVWGRVQKLLNRSRLYQFVVSRAAVRQ
                     EPDADHYVLRVPPEEYRQNLESFIAEIRAAGAVPILLTAPRAAVLTPILVAHRQVARI
                     EDAYRLHDEYGEITREVARETGAPLLDLARIFEAERQTVLFSDDGIHQNKDGRRRIAA
                     EIYKLLVDLNATRSPEAR"
     gene            complement(5097..5918)
                     /locus_tag="PK634_05690"
     CDS             complement(5097..5918)
                     /locus_tag="PK634_05690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOW97262.1"
                     /translation="MKPAARFLLAAGITLLALAGAGYWRWRVAWRVPGPDSRVSIPVF
                     VLHRVLPGEATEYIISPDRLKKLVRELNRRGFTPVSLGQLHAALHDRGPLPRKPAMIT
                     FDDAYLDMYVHALPVLRKHGWPAVFFAPTGKMSDPPAERVVWGDGPDPRAMQWPELIA
                     LKEAGMEIGSHAWTHINLAKALPETVNAELETSRREIAERIGVAPMALAYPGGRHSRE
                     VRRAAAEAGYRLGFLSGGGPIPLGVEDLYALPRVHVPGYADPAAIVSALPENVWR"
     gene            complement(5919..7811)
                     /locus_tag="PK634_05695"
     CDS             complement(5919..7811)
                     /locus_tag="PK634_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOW97263.1"
                     /translation="MNIFYVIFSVLVTGGIFAWLFSHVSLAEVAGLLRGVDPRGVALF
                     VLASLLMSVFRAWRYRIILRVSGPAPGTLPLFLAVIVRNTFSDLLPARLGSLVYIYVA
                     TARLGIPFGAASSSFALAFVFDMIALAPLIAWAAVGLGGASVWVLAGFGLVLAAGMIA
                     VLHWLPALVGIVGRRVAAFSWLGHERGPRWAKALEDVACELRAAREAGIYGRVFVLSL
                     MVRLFKYAALYFFLFALVAPLGYGWAALPVPRVLAGLCAAEAAASLPVSGLAGFGAYE
                     GAWAMAFQLLLFPAPLARATSIAHHLFTQLYGYGSGAVALLLLLLPFWRRARPPEREA
                     LESAGRFAARLAAALTVAAGMAVLLYRVCPEARAEAAPVSAGDVSALENLAGRVRGRV
                     VFQRPDGIYVVRLGARDPERIVSPGEFPRWSPDGRDIAFLQSNRVMRLRLDGGEPEAL
                     ADAFAPRALAWHPSGREIYFTDGLLIRAVNVETRAARDVLSGHVFRELDVAPDGRLAA
                     TVRGRGVSIMGFDPGAGRSWRIAGGCSASLSPDGNRVTNNEGDHRRLSIRDAVSGARL
                     GTVNAPAGMAFDNQWWSNDPNWLASRSEGLFEDVFVHDLLKNEAVRVTFVGQCDRPDL
                     FIESGD"
     gene            8041..9795
                     /locus_tag="PK634_05700"
     CDS             8041..9795
                     /locus_tag="PK634_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97264.1"
                     /translation="MNSRDSRRIILPVLLLAAAGLRLLYFGEIRGRADFIHPGLDAGY
                     HDYWARGIAFDRWTPPPDQPDPLIRQLPYFRPPGYPYFLSAIYRLIGAAPAPARLVQF
                     ALGVLNVFLAFWFTRRWFGEAAGLIAAALAGTYWVFIYYEGELLEPVLLITLTWLALL
                     ALGQWTADGRPAWLLGAGLALGASALARPNALAWLPAVALWLAWRIGDWKKAARPAGL
                     LVAGTLLAVLPVTARNYIVARDVVLISSNGGINLLLGQDPEAVADHASSETGNWSCFD
                     YPALLAKASAKAGRPLKASEASRWFARKARQQIIGRPGVTARLLALKTLLFWGPREVS
                     NNKIEELERAASPVLRRLPLSFSFVLAWGLVGGALTWRKRRDTLPVAALLLLLIAAYF
                     LSFLPFIAAGQYRAPLTPLLMVFAAVGLVETGRLAAGSKPVEAVAGLAAALALWFVIG
                     INYTGYCPSEARWHLGRAISLERAGDLAASETAYREVLRAKPDLALAHNRLGVILAKQ
                     DRTAEALKCFEAAVAADPALTDARFNLGLALALQNRLAEAIPEFEAVLRIQPDHADAR
                     HNLEQARRLLGSPKANEP"
     gene            complement(9848..12091)
                     /locus_tag="PK634_05705"
     CDS             complement(9848..12091)
                     /locus_tag="PK634_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97265.1"
                     /translation="MQIVRRFARSEAAWLGIILLAALVLRVLYLAEARRDPSFAMPAS
                     DAAFHDYWARALVTGDWTPPPETPDPRVPSTPFFRPPGYPYFLAAAYRILGGRYMAPR
                     ILQMGLGILNVLLAFRLGRKMFDSAAGLVFAALMAVYWSFIYFEAEFLEPVLLVTLGL
                     LMVLLLASWLQSPRVWMAAAAGLCLGLYALTRPNVLVFAAALPLWMLWITHRRRGEKR
                     IRWLHFGLLAAVALATILPATIRNYRRGHDRVLICSNGGINLYIGNNPAANGIFVEPP
                     ELRPFRSVFDWPALVKRLEGEAGRPLRHSQVSSMLTRAAARFAWRYPRRALELAGRKT
                     LLFWGPVEVGNNKEDHWERHFSPLLRRLPGNFTAALALALVGFWLWRRRPAAPAGSRA
                     GAAESVALILLLVLSLFASYLPFFVAGRFRAPIVPFLLLFAAYALSEWWKLFREQRMR
                     EAVPGLAAAVILYLVLGVNWAGHRPQEADYHYFRGLAFERLGQTQPAMSELVLAIRQR
                     PGHAPTLKLLGGLLLNIQRPEEAVECLDAAARLLPDDAKVRHLRGVALARTGRLPEAR
                     AEFEEALRLHPGNADVLFNLGMTLARMGEFARADEILALGLAAAPDRTDLLVTRAMVA
                     WKQGRAREALDLVGKADRLKPGDPEILNQRAWMLAVAPEPSDRRPEEAIRLAAEACRL
                     IGRDHPPFLDTQAAALAAAGRFEEAAQTARRALELGAGSPEEFQREVQERLALYQAGR
                     AYVEPAR"
     gene            12127..13407
                     /locus_tag="PK634_05710"
     CDS             12127..13407
                     /locus_tag="PK634_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOW97266.1"
                     /translation="MALTDMRIFRQLVIVLLPALWLGGLPAEAATPRAFRLLSESNTE
                     AVGLTPAGTLTWSNQLPAGRFQVEQNFNLSTGQWHSFISGPATATWHSLKLWDFNAPT
                     GMVYIPAGDYVRGDGYAWDLRSLPVHTAWVSAIYMDRREVTKALWDEVRDWGLTNGFT
                     DLSVGVGGGKRVMVTVTNDGVPTNIWVNIEAGPEHPVVYITWYDMAKWSNARSLRESL
                     TPPYYTDTALTTVYRTGQVDLATNQVNWECTGYRLPTEAEWEKGARGGLIQHHYPWPS
                     TGTSLPDVIDGTRANYWESRDVFDQGTTPAGYYDGSQVVTNELGEIQPVQDMANAYGL
                     YDMCGNVWEWCWDYYSFTSYTWAVQNTNLRDIRGPPFHDTAQPYRVDRGGSWRTSTNA
                     LNDMRCVQRWFETAGWASPSLHRGFRTVRRPVTP"
     gene            13415..15151
                     /locus_tag="PK634_05715"
     CDS             13415..15151
                     /locus_tag="PK634_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97267.1"
                     /translation="MRSIRQIAGRAALASLVPLTLFGIAELALRVLDLGYPTEFLLET
                     TVRGRAALVDNPFYGYRFFTPALARNPAPILTSRDKPADTVRVVVLGESAAQGDPLMQ
                     FGLPRMLQTVLNGAAATGRFEVINAAMTAINSSVIVDIARETTACRPDLYVLYIGNNE
                     VIGPYGPGTVFTALPGLTRMAPWRVSLTGWRLAFFLREGLTSLFPSRPGSSKWSGLAM
                     FSERPVPFGDRSLGPMYDLFERNIRAIVNCARDAGAQVILSTVAVNLSDCPPFGGPEA
                     ASAFALAREQQDRAGYKKARDLDIYRFRTDGMLNERLRKLAGELRVPLADAERAFDDA
                     DADADFFVDHVHFTFSGTHRLASTIAGAVAGLYPGRFPPAAWPDEARCRGELFYTPWS
                     ELQMAMIMLDRRRQPPFDRQPDNAACLLRLEDEAKRLSVQIAQTDPAELASRYEALKS
                     KNPEDFFLPFDWGAILSDRSRWADAAEVLLPALELVPLHFEARKLPALALCRSGKTEE
                     AAGVLLGPEKRRGYFVADYALEIMGLLAAEGQKEQAAALGRALLEKDPRLPWHECIEA
                     MTKRLEGRRADE"
     gene            15148..16743
                     /locus_tag="PK634_05720"
     CDS             15148..16743
                     /locus_tag="PK634_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97268.1"
                     /translation="MKITRNELLASAALTLLVFFLYAPAVRFAFVDYDDPIYITENSW
                     VQQGLSAEGFRLSLREWVSGNWHPLTMWTHMLDAELFGLNPAGHHAQSILWHAANTVL
                     LFLLLSRIAGGFWPGLFIAALWAAHPLNADSVAWISERKNLVSTFFWLATIGLYIRFT
                     RRPSPHALMAVIAGFALALMAKPMPVTLPFTLLLLDFWPLRRVEGIGRSAWPTWKKLA
                     LEKLPLFVIGAVFILTTLKTQVLAGATGMSSFLSLHARLAWLPIFYLDYLRLFFWPRD
                     LCVLYPAPTAAPSIPASAGALLILLLITAGALVLARRRPWLAAGWFWFLGTLAPVIGL
                     VSVGLHRIADRYLYVPMLGLLLMVVWRLFDAASKRWRPAAFALAAAVVLVLGLVTRHD
                     LPHWRNSEALFRRALAVTRDNYAMHYNLGRQLTLQRKFDEAIAEFREAIRLRPDYALA
                     HNNLGWTLCLRDDNEEALPSFRESLRLMPDNHQARFNLGLALMRLGRYAEAREQLEIL
                     LQQAPAYPEAQDVYQQLLKAGRS"
     gene            complement(16782..>17368)
                     /locus_tag="PK634_05725"
     CDS             complement(16782..>17368)
                     /locus_tag="PK634_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97269.1"
                     /translation="YCRGVARFLAGDPAGALADYDRALEIMPHYVKCRLSRAAILVQQ
                     ARPEAALADLDQAVSMEPTTHALAFRGYVLSRLKRYAEARESLDQAVQRDPQQPKAWD
                     ALAWLLATAEDPAVRDGARAVQSAEEAVRLQPHDPAHLDTLAAALAETGRFDAAIATA
                     ERALALAREKQAGDLAERIRVHAEIFRKGIPVRE"
     assembly_gap    17369..17378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17379..18691)
                     /locus_tag="PK634_05730"
     CDS             complement(<17379..18691)
                     /locus_tag="PK634_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97270.1"
                     /translation="MKSSLLKAGYALLLALTVGLVYQNTLDGPFVLDDQDSIVGNPAI
                     RRLESLSEALNPPPAECTFCTRPFINLTMCANYALGALNPRGYHLTNMAFHLAAVMAL
                     FGLLSRAFRRLGFADAEAAQWSFFCALIWAVHPLATAAVNYPSQRGELAVGLFFFLML
                     YGLDRAHGMRLAGSWPSMSMDKVCEGERPREPKVAILWLALSVFACLLGMGSKESMIV
                     APVLALIYGRIFLAPSWKELLRGRVPYFAALAATGLWPVYRHVVYSPHLAHAGLRADT
                     WWDYLFTQAWGLNRMIRLVLWPHPLVFDYGSILIRSLGEVWIPALGLLLLLAATGWAL
                     VRQPPWGFAGLCFFSFLAPSVFFPAIGQTIAEHRMYVPLACAAALIVMGLRNLSRFWK
                     WPTAAAPVLLCVAALSLAVASRHRNAAYRSEVALYTDSIARWPINA"
     gene            complement(18833..19396)
                     /locus_tag="PK634_05735"
     CDS             complement(18833..19396)
                     /locus_tag="PK634_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW97271.1"
                     /translation="MRKIIIGLVALAAALSAMGATGWYQDYVLISLNGDPAGYYWIGG
                     DPTFGTQFNGAFLGNVGAANSVRIGADMRYWSDTADRDGGAFYYTLDGTGYQEILWTQ
                     TYLGGNDYQGTEPATHVVVQGGSPGTHTLTVWAKSWGTGQGDSWLSGVSGENYSATFF
                     IQSIPEPTTLVLVGLGVAGLLAYRRRR"
     gene            complement(19467..20030)
                     /locus_tag="PK634_05740"
     CDS             complement(19467..20030)
                     /locus_tag="PK634_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW97272.1"
                     /translation="MRKLIVALVALMAAVSAMGALGWYSDYVLVSLNGGADGYYWIGL
                     DPTFGTQFNGANLGSVVQGQMVELGADMRYWSDTADRDGGAYYRTIDGTGFQEVIWTQ
                     TYLGGNDYQGLAASSDNVAAGLAVGTHTLTVWAKSWGTGQGDSWLTGVTGENYVATFG
                     ITAIPEPTTLVLVGLGVAGLLAYRRRR"
     gene            20339..21532
                     /locus_tag="PK634_05745"
     CDS             20339..21532
                     /locus_tag="PK634_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOW97273.1"
                     /translation="MNAQPIRSITLLGSSSGRNAGDAALIAAIMDNVDRALGRRVRYE
                     IPTPRPDYLRHHYPNDVHPVGMMPWQLSVKMLGLPTLRSILRTDLTLVFDAILFDRSL
                     YNPLFNFLSTLYLLLPLARKLGKRMGCYNVSVGPIRTRAGARMLRRVLGCMDFVAVRD
                     TEALDLFREIGLEHPRVRLTADAAIGAPAADRARVDEIFRKAGLTPGEPVLGININRY
                     LDTWASRSRPPMGAEAFLGTYAGALRRVWRELRVPLVFACTQHHDVEITQELMRRLEP
                     DVRAALIANRDYDHAETKGALGRLDLLCGMRLHSLILASSGLAPIIGIAYQPKVNYYF
                     NTLGLGDFVLGFDEFTEEKLAAHILRGWRERARLRATLEQRIPAMTLQAARTADLVAG
                     MERGV"
     gene            21567..21923
                     /locus_tag="PK634_05750"
     CDS             21567..21923
                     /locus_tag="PK634_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOW97274.1"
                     /translation="MRDHTKLRAFELADQVVMLTYQITRHFPKAEAYGLTAQMRRAAV
                     SIASNIVEGCARGSQPDYLRFLEIAFGSLRELHYQLTVARRLGYCDEAGFIEGDTIML
                     EAEKVLGALIRALRRN"
     gene            22072..24546
                     /locus_tag="PK634_05755"
     CDS             22072..24546
                     /locus_tag="PK634_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97275.1"
                     /translation="MSSQNVEKGHARAMVPGDHLQLLYHFELLRDMLAGRVPWFHNVY
                     EFNTGDDAERYRPSAYFAPFSLPYALLAAAGGRALAWNLVGFLSLWLTALATALLAGR
                     FATSRLAVYGAALAALLIPFRWVNLLGGSPAGLAMAWVPLTALGVDLAVRDRRMGGGL
                     LAGLAILLACWSDTQVFFFCTISLPLWLLLSWIGSAETGTRDLKRVAAALAPIALFLL
                     AAFFYRYLLHEHLSSSGMGRGRSLAEVKLFSPTWPGFWQWAAEGLQSHIYIGWMLPLL
                     LLAGGYALRRNVRTGAPGSFSTFFVFLLCAAAIGDVMILALGVHGPVDGLALKIVRKL
                     LPPYSMVRQTAKIFCLVPTLAALALALGLEGWLAFATARPRLARGAAILGLLLLAAEM
                     RGQVRPTVCLLDSGQGAYAAVAEDARGAGNPPGALVLPLWPGDSSWASIYEYYAQMHG
                     IRMINGYSPVVRRDYVEQVFRALESANQGWLTDEQLDLLARMKTGYVLVHENAFPEKV
                     SPFPVGLTLDRLRRHPRLSFLGQAENVWAFRIEPAAAPRAPAPPGPGCPVAFPARRWE
                     WESVESGNGTAIEDASAGGGAFWRASDPGAWAGTRPVRAAPDPALYWLVRGRGRGAVE
                     AITLSGEAALPASRLAFDSTEWTWQSIPVSGHDTYGPLQLRFARAEGSVDLDVAMLCS
                     GSWPQAWPPGDRVEIPAACLFHGGHSGGDEVIFRARHDPESAILYGPKLPLPPGLYRM
                     ELVFSSGAPAGEPLGRFNVRTREEDESGAVRVTAGRPCVTTWRQTGSLPVNAVFVYAR
                     NHDVKVVGLIIEAVHAAQEGLALPGL"
     gene            24509..24949
                     /locus_tag="PK634_05760"
     CDS             24509..24949
                     /locus_tag="PK634_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97276.1"
                     /translation="MLRKKDWLYRVYSVGSVFWLLTYQSIPGCSDTGPSGPPPVGADI
                     HGKWKGEFYRTDRGYQIEVTARVEQESDAVIIDTSKTEGVAQLLTGSISSNGYLRLTD
                     ANTGEKWTSYFGPASETNLVVADYAETPTIEHPEPGLYVLALHR"
     gene            24980..26638
                     /locus_tag="PK634_05765"
     CDS             24980..26638
                     /locus_tag="PK634_05765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOW97277.1"
                     /translation="MKRDLARLSDESFDLVIIGGGVYGLSAAWDAALRGLRVAVLEKG
                     DFGAATSSGSLKLIHGGLRYLQHLNFARMRISINERHRMLRIAPHLVHPLEFVMPCYG
                     HAMKGPEAMRLALLFNDLMSFDRNRGVDRAHAIPNGRTLSPAECLDRLPGIRREGLTG
                     GAVFYDAQMHNSERLTLSFGLSAAEQGAALANYAEVTGFHVEQDSIRSVIARDRLSDR
                     MFHVAGRLFLNLTGPWADITSRLLTSPSPDRRVVRSKGIQLVTRALPPAAAFPIESRQ
                     RDLTAVIKRGGRNYFVTPWRGLSILGTTDTLYEGSPDEFAITEADIGEFLAEINALYP
                     AGLERKDVRFWIGGMRPLGDEDTNPEHAKASHKSQIRDHAREGGPSNLISAVGVKYTV
                     CRHIAENLVDLAVRRLGAAAGRCRTRETPLAGGDTGDFESFLAESRRVAPAGTSEHLA
                     RNYGTRLRSVLALVSGDPSLARPLADSTEVLRAEVVHAVREEMAMRLDDVVLRRTGLG
                     TLGHPGRPTLEECARLMAAELGWSPARQADELAAVERVFTPPAG"
     gene            complement(26660..>27106)
                     /locus_tag="PK634_05770"
     CDS             complement(26660..>27106)
                     /locus_tag="PK634_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97278.1"
                     /translation="ASAAAAGSEEPAAAGNPEEPAAAAGQEELGIAAYEYQGELFVDT
                     FCETLPLNDGAGGFVSNPDNMHETLKFVFPASFRDQISRVTVFTSDGRFFDEFWREFP
                     DEEDGRPRYYGTKPVELYPANMYLRVLMNDGSCRSLQHPNPQQRFT"
BASE COUNT         4294 a         9494 c         8951 g         4357 t
ORIGIN      
        1 gatatagcgg ttgagcgtgc gcaccctaga ggctccccgc gggaccgccg cgcgcggccg
       61 ccaccgtgtt gaacagcagc atcgtgatcg tcatcggccc caccccgccg ggcaccggcg
      121 tgatccacga cgccttctcc cgcacctcgt cgaacgccac gtcgcccacc aggcggtatc
      181 cccgcggggc cgaggaatcc gccacgcggt tcatgcccac gtccaccacc acggcgccct
      241 cgcggatcat gtccgcgcgg acggtgttcg gacggccggc cgccgcgatc acgatgtccg
      301 cgtgccgcgt atgctgcgcc aggtcgcggg tggcggtgtg gcacagcgtc acggtcgcgt
      361 tgccggcctc gcccttctgc gaaagcaggt tggcgagcgg ccgcccgacg atgtggctgc
      421 gcccgatgac caccgcgtgg gccccggcca ccgggatccc cgcgcgccgg agcatctgca
      481 ggatgccgtg cggcgtgcac gggaggaagg ccggcaggcc cagcacggcc cgccccacgt
      541 tgaccgggtg aaacccgtcc acgtccttgg ccggatcgac cgtctcgaga acctcctgct
      601 ccatggaccc gtccggcagg ggaagttgga ccaggatgcc gtgcacccgc ggatcgcgat
      661 tgaattccga gacgacccgg aggatttccc cccgcgaggc cgacgccggc aggcggacct
      721 cggaggaatg catgcccagt tcgccgcagg ccttttcctt ggccgtgaca taggcgcgcg
      781 aggccgggtt atccccgacc agcaggacgc cgaggcccgg cacaatcccc ccggcgcgca
      841 aggccgccac ctgcgccgcc aattcggccc gcagttgccg cgacagggct ttgccgtcga
      901 gtatcttcgc cgtcatgatc ctgaacccca cgagtcggcc ggcattatcg caaaaaattg
      961 ccgcttgggg ataaaaaacc gcggagccgg acgcggggta ttccggccga gaacgccccc
     1021 gccttgacaa gacagggccg cgtgcccacc ctatcgcgca tgcaggaacg catagcacag
     1081 cgaccttccc tcgtcgacgc ccttcggcag atcgccgccg gacttctggc cgccgcgacc
     1141 ggcatcctgc tgctcctgtt cctggtctcc tccctgtacg gcggcttcaa gatggccacg
     1201 ccgctgggtt cgttatcctg ccggcatccc gggccgctgc tgctgtggac cgcgctctgc
     1261 gccggggtgt gggcgctgat gtcacgcccg gcgacgcgcg gggaggacga cgcggcgcct
     1321 cctttcagcc gggcgggcgc catcgggctg ctgctggccg cgaccgccct cctgtggctt
     1381 cgcttcctgt accggtccta cgagccggtg ttcggccacc gcccggtgcc gacggttccg
     1441 tttcttttca cgcatctcct ttttctgttc tccggcctgg ccgcgctcgg ggtctggcgg
     1501 caactccggc ggaccgaggc ggagccggcg gcgcttttcg cggccttgtt cacggccttg
     1561 tctccgctct ggtttcacgt ctccgcgccc gtcgtcgcag gctacgcggc catcgcgctg
     1621 ctgtggtggg gcgcgccccg cctggagcgc gccccgggct ggctgcttgc gacggcgctc
     1681 ataccggttg cggccgccgt tctgcgccac tggggctggg ccggaaacag gctgacggat
     1741 cagttcgctg ccgcggctcc cggcctttgc gcctggctgc tcttgccggg gctggccgga
     1801 tggtggttca gtcggcgaac ccggcggccc tggatgcggg ccgccctgtt ggcgtgcgcg
     1861 tggagcgtgg tctgcgccgg cgcgcgcggg gaggccggca gcctcggcgc cgtcctgggg
     1921 ctgcccctga tcgcaagcct gctggccgtg ggcggcgggt ggttgctgtc cgccgtgccg
     1981 gcggaacgcg ggtggctggc gcgcaatgtc atcatgatct gcctggcggg catcctgctc
     2041 ggaatggtga gggacggcct ggaccgcagc atccgttccc ccttcgcccc gccgctgccc
     2101 ggaccggaag ccccggccgc cgcatccgag ccggaggagg cgccggagtg aaaccggccg
     2161 gcgaccggcg cagcctggcc atcgcggccg ccctgctggg gctctgcctg ctggccttct
     2221 tcttcaagta cgaactccgg tacgggccgg cgccctacct tttctacggc gacgcgaaca
     2281 ccggccagcc caactccgac gccgtgacgt ggaacatcca cgcgctgcac ctcgtggagg
     2341 gacgcgggtt cggcgactac ctcaagcagt tccggtcccg gaattacgtc ccgccgggcc
     2401 atccgcttat cctcggattg ttctactacc ttttcggcaa ggatccggaa gccgccggct
     2461 ggtgcgtggc catcctgttc agcctgctgc cgtgggtcac ctacctctgg gggcgcgaga
     2521 tgtgggggcg gcgcgtcggc ctgatcatgg ccgcgctcgc cgcgctgcac gcgccgtaca
     2581 tccacatcgg cttcagcctg atgtccgagc cgacgacggt attcaccacg gccgtgtccc
     2641 tgtggttcgg cgcccgcatg attcggcggc ccggactgtc ctcggcggct ctcgccggcc
     2701 tggcgttcgg cttctcggcc ctggtccggc cttccgccct ggccttcctg tggggcctgg
     2761 ccgtcgcgct cctgatggcg cggccgccgg ccggcggcga agccgttcga acgcccggcc
     2821 gcccccgcct ggtcgcgctg gccgcgttga tcctctgcac gctggttccc ctgggcctct
     2881 ggcaataccg caactaccgg gtccacggcc gggcggccgc cgtctactcg tccatcagcg
     2941 cgcggcacgc ctggcacggg gcgcatccga agtacggccc gggcttctac agccgggaag
     3001 cctggcacga gacgctgtgg cgcgagccgt acgccccgga gctggccatg atccagcggc
     3061 ttcaggccga gacggacgag ttcatccgcg ccgaccggtt caagtactgg atgtcctgca
     3121 tctggcggat caaggtgctg ttcccggaat tcatgcaccg cgaggccggc atccgctgga
     3181 gccacggcgg catcggccgc atccatttca tggcgctctc cgcgatggca atcctcggac
     3241 tgctgcgcgc cctgcccctc cgcgtgcgcc tctcgggccc ggacggctcg acggatctgc
     3301 ccggcgccct gtgggcggcc agcctgctca tcggcctggc cgcggccctg gccggcgccg
     3361 gcatttacgg cgcctcggac cgctaccggt ggccgctcga gttcgcgctg atcccgttcg
     3421 ccgccctgtt cctcgatgag ttgctccgct tgagccgcgc gccgttcttc acgaggggcg
     3481 cgctgaccag ttcatggccg ccgctcccgg cgccgctgcg cttcgcccgg cggctgggcc
     3541 ttgcggcgct ggcgcttgtc gtgctggcgt acgccgccgg cctggcgcgg gcgcggcgcc
     3601 atcccgattc gccggaggat gccgcgcccc ggctgacgga agcgcaggtg ctccgcggca
     3661 tagaccgggt cggactgcgg acggagttcg acggccagtc gccccgctgg atcacctatg
     3721 accaggtttt tacggagcag gccgcgaact tcggcgcgct gaccggcctc aacatgaagc
     3781 tcgtggcctg gtggggccgc cttcgcttcg tgcagaccga ttcccggggg ctggcccggg
     3841 ccggctatgt catcctcgac ccgcggcccg gcgatttcgg gggcgcgagg ctcccgatcc
     3901 gccgcgcccc gaacgcgcgc ggcccctacc gccacgtgcg ggacggcgac gcggtgacgc
     3961 tgctggcccg cctgtactac gagcccggcc ccatccagac tccgctcctg gtcttttatg
     4021 acatcctgcc cgggcgcttc gacggcccgg cccccggagg cgccgaacca tgacggattc
     4081 ccgcgcagcg tggtggtgga agagattgct gctcgccatg gttaccgccg gcgccaccgt
     4141 ggtcgcgggc gaactggcgt tgcggctggt ccgcttttcc gcgcccgtcc agccgatcac
     4201 catcgtcgat cgcgagggca agagcgaccc gggcgataac cgggggctga tcccggattc
     4261 ggagctccag ttccggttca atccgggcgc ggagtggcgc gggcggcgga tcaactcgat
     4321 gggtttcctg ggccgcgagg tcgaatcgaa caaggcgccg ggcacggtgc gggtcatctg
     4381 catgggcgac tcgtgcacgg cgacgggcaa cccgccgtat tcggatttcc tgcacgagct
     4441 gctgaccggc tcgccgccca cgccgcagcc gtgggaggcc ttcaacatgg ccgtccatgg
     4501 ctattccacc gagcacggcc tgcggctctt ccggcgccgg acgaggcacc tgcagccgga
     4561 ttacgtcacg ctgtattacg gctggaacga ccattggcgc gacgacctca ccgaccggct
     4621 caagctcgcg cgccgggccg ggcctgtctg ggggcgggtc cagaagctgc tgaaccgctc
     4681 gcgcctgtac cagttcgtgg tcagccgcgc cgccgtgagg caggagcccg acgcggacca
     4741 ttacgtcctg cgcgttccgc ccgaggaata ccggcagaac ctggagagct tcatcgcgga
     4801 aatccgcgcg gcgggcgccg tgcccatcct tctcaccgcg ccgagggccg ccgtgctgac
     4861 cccgatcctg gtcgcccacc ggcaggtcgc ccggatcgag gacgcctacc ggctgcacga
     4921 cgagtacggc gagatcacgc gcgaggtggc ccgggagaca ggcgccccgc tgctggacct
     4981 ggcccggatc ttcgaggcag agcggcaaac cgtcctcttc agcgacgacg ggatacacca
     5041 gaacaaggac gggcgccgcc gtatcgcggc ggagatctac aaactcctgg tggatctcaa
     5101 cgccacacgt tctccggaag cgcgctgacg atcgccgccg ggtccgcgta gccgggcaca
     5161 tgcacgcgcg ggagggcgta caggtcctcg acaccgagcg ggatgggccc gccaccgctt
     5221 aagaatccca gccggtatcc ggcctccgcg gcggcccggc gcacctcgcg gctgtggcgg
     5281 ccgcccggat acgccagggc catcggcgcg acgccgatgc gctccgcgat ctcgcgccgg
     5341 ctggtctcca actcggcgtt gacggtttcg ggcagcgcct tcgccaggtt gatgtgggtc
     5401 caggcgtgcg acccgatttc catgcccgcc tccttcagcg cgatcagttc gggccactgc
     5461 atggcccggg gatcggggcc atcgccccac accacgcgct cggccggcgg gtcggacatc
     5521 ttgcccgtcg gcgcgaagaa cacggcgggc cagccatgct tccggagcac cgggagggcg
     5581 tgaacgtaca tgtcgaggta ggcgtcgtcg aacgtgatca tcgccggctt ccggggaagc
     5641 ggaccgcgat cgtgcagcgc ggcgtggagc tgtcccaggg aaacgggcgt gaatccgcgc
     5701 cggttcagtt cgcggaccag tttcttgaga cggtccgggc tgatgatgta ttccgtcgcc
     5761 tcgcccggca gcacgcggtg cagtacgaaa accgggatcg agacgcggct atccggcccc
     5821 ggcacccgcc aggccacgcg ccagcgccag tatcccgcgc ccgcgagggc caggagggtg
     5881 atgcccgccg ccaggagaaa tcgcgctgct ggtttcattc agtcgccgga ttcaatgaaa
     5941 aggtcgggac gatcgcattg ccccacgaag gttacgcgaa cggcctcgtt tttcaagaga
     6001 tcgtgcacga agacatcctc gaacaggccc tcggaccggg aggccagcca gttcggatca
     6061 ttggaccacc actggttgtc gaaggccatg ccggccgggg cgttcaccgt gcccagccgc
     6121 gccccggaca ccgcgtcgcg gatcgacagc ctgcggtggt cgccctcgtt gttcgtgacg
     6181 cggttcccgt ccggcgacag gctggccgag cacccgccgg caatgcgcca cgagcggccg
     6241 gcccccggat cgaagcccat gatggacacg ccgcgcccgc ggaccgtggc cgccagccgc
     6301 ccgtcggggg cgacatccaa ctcgcgaaac acgtggccgg aaagcacgtc ccgcgccgcg
     6361 cgggtctcca cgttcacggc ccggatcagc aagccgtcgg tgaaatagat ttcccgaccg
     6421 gacggatgcc aggccagcgc ccggggcgca aaggcgtccg ccagcgcttc cggttcgccc
     6481 ccgtcgagac ggagccgcat cacgcgattg ctctggagaa aggcgatgtc ccgcccgtcc
     6541 ggcgaccagc gcgggaattc gcccggcgac acaatccgtt ccgggtcccg cgcgcccagg
     6601 cgtacgacgt agatgccgtc cggcctttgg aacacgaccc tgccccgcac ccggccggcc
     6661 aggttttcca gcgcggagac gtcgccggcc gacacgggcg ccgcttccgc gcgggcttcc
     6721 ggacagacgc ggtaaagcag gaccgccatg ccggcggcga cggtcagcgc ggcggccagt
     6781 cgcgcggcaa accgtccggc cgattccagg gcttcccgct ccggcggccg cgcccggcgc
     6841 cagaagggca gcagaagcag gagcagcgcc acggcgcccg acccgtaccc gtagagctgg
     6901 gtgaacaggt ggtgggcaat gctggtcgcg cgggccagcg gcgcgggaaa cagcaacagt
     6961 tgaaaggcca tcgcccacgc gccctcgtac gcgccgaagc cggcgagccc ggagaccggc
     7021 aggcttgcgg ccgcctccgc ggcgcacagg cccgcgagga cccgcgggac cggcagcgcc
     7081 gcccagccgt atcccagcgg cgccacgagc gcaaagagga agaaatagag cgccgcgtac
     7141 ttgaacagcc tgaccatcag cgagagcacg aacaccctcc cgtatatccc ggcctcccgc
     7201 gccgcgcgca actcgcaggc cacgtcctcc agcgccttcg cccagcgagg gcccctctcg
     7261 tgacccagcc acgagaacgc cgcgacgcgc cggccgacga tgccgaccaa cgccggaagc
     7321 caatgcaaaa ccgcgatcat gccggcggcc aggaccagcc cgaaccccgc gaggacccag
     7381 accgaggccc cgcccaaccc gaccgccgcc caggcgatca gcggcgccag cgcgatcatg
     7441 tcaaaaacga acgcgagggc aaagctcgac gaggccgcgc cgaacgggat gcccagccgc
     7501 gccgtggcca cgtagatgta gaccagggag cccagccgcg ccgggagcag gtcggagaac
     7561 gtgttgcgga cgatcaccgc caggaacagc ggcagcgtcc ccggcgcggg cccggagacg
     7621 cgcaggatga tccggtaccg ccaggcgcgg aagacgctca tcaagagcga agccaggacg
     7681 aacagcgcca cgccccgcgg gtccacgccc cggagcaggc cggccacctc cgccagggac
     7741 acgtggctga agagccacgc gaagatccct cccgtgacca ggacgctgaa gatgacatag
     7801 aaaatattca cgacgatatc cgggcggcgc tccgccctcg cgcattatcc agtccgcgcc
     7861 ggcccgggca accatttttc acggcttgcc ttctcgcctc gcgagcgagc aggaaggaat
     7921 gcccggttac gcggcagacg gccgccagga agcacgatcc agaccggcga actctgtcgt
     7981 ttcgcttccc ggcgtcaatt ttttctttgt caaaccggct gcccgccgta ccctgcccgc
     8041 atgaattccc gggacagccg ccgcatcatc ctgcccgtcc tgctgctggc ggcggccgga
     8101 ctgcggctcc tgtacttcgg cgagattcgc ggccgcgcgg acttcattca tcccgggctc
     8161 gacgcgggct accacgatta ctgggcgcgc gggatcgcgt tcgaccggtg gacgcccccg
     8221 cccgaccagc ccgatccgct gatccggcaa ctgccctatt tccggccgcc gggctacccc
     8281 tatttcctgt cggcgatcta tcgcctgatc ggggcggcgc ccgcgcccgc gcggctggtc
     8341 cagttcgccc tcggcgtgct gaacgtattc ctggcgttct ggttcacgcg ccgctggttc
     8401 ggcgaggcgg ccggcctgat cgccgccgcg ctggccggga cctactgggt cttcatttac
     8461 tacgagggtg aactgctcga gcccgtgctg ctgatcacgc tgacgtggct cgcgctgctg
     8521 gcgctcgggc aatggaccgc ggacggcagg ccggcctggc tgctcggcgc aggcctggcc
     8581 ctgggcgcgt ccgcgctggc gcgcccgaac gcgctggcct ggctgcccgc cgtcgcgctc
     8641 tggctggcgt ggcggatcgg cgactggaag aaagcggcgc gccccgccgg cttgctggtc
     8701 gcgggaacct tgctggccgt cctgcccgtg acggcgcgga attatatcgt cgcgcgggat
     8761 gtcgtcctga tctcgtcgaa cggcggcatc aacctgctgc tgggccagga tcccgaggcc
     8821 gtggcggatc acgcctccag cgagaccggc aactggagct gcttcgacta tcccgcgctc
     8881 ctcgcaaagg cctctgcgaa ggccggccgg cccctgaaag cgtccgaggc gtcgcgctgg
     8941 ttcgcccgga aggcgaggca gcagatcatc gggcggccgg gcgtgaccgc gcgcctgctc
     9001 gccctcaaga cgctcctctt ctggggcccc cgggaagttt ccaataataa gatcgaggaa
     9061 ctggagcgtg cggcctcgcc ggtcctgcgc cgccttccgc tttccttctc cttcgtgctg
     9121 gcctggggcc tggtcggcgg cgcgctgacg tggagaaagc gccgggacac gctgccggtg
     9181 gccgcgctgt tgctgctcct catcgcggct tacttcctgt cgtttctccc cttcatcgcg
     9241 gccgggcagt accgcgcccc gctgacgccg ctgctgatgg ttttcgccgc cgtcgggttg
     9301 gtcgaaaccg ggcgcctggc ggccggatcg aaacccgtcg aggccgtggc cggcctcgcg
     9361 gccgcgctgg cgctctggtt cgtcatcggg atcaactaca ccggctactg tcccagcgaa
     9421 gcgcgctggc acctcgggcg cgcgatcagc ctcgaacgcg ccggcgacct ggcggcctcg
     9481 gaaacggcgt accgcgaggt gctgcgcgcc aagccggacc tggcgctggc ccataaccgc
     9541 ctgggcgtga tcctggcgaa gcaggaccgc acggccgagg cgctgaagtg cttcgaggcg
     9601 gcggtggcgg ccgatcccgc cctgaccgat gcccggttca acctcgggct cgccctggcc
     9661 ctgcaaaacc ggctcgccga ggccatcccg gaatttgaag cggtcctgcg aatccagccg
     9721 gatcacgccg atgcgcggca caacctggag caggcgcggc gactgctcgg atcgccgaaa
     9781 gcgaatgagc cgtaggcggg gcgcgtcggc tcaacgcgcc gaccgcggcg gcgtgggcca
     9841 agccgcccta ccgggccggt tccacgtaag cccggccggc ctggtataag gccaatcgct
     9901 cctggacttc gcgctggaat tcttccggcg accccgcgcc gagttccagc gcgcgccggg
     9961 ccgtctgcgc cgcctcctcg aagcgaccgg cggccgccag cgccgcagcc tgcgtgtcga
    10021 ggaacggcgg atggtcccgg ccgatgagcc gacaggcttc ggcggccaac cgtatcgcct
    10081 cttccgggcg gcgatcggac ggctcgggcg ccacggcaag catccaggcc cgctggttca
    10141 atatttccgg atcgcccggc ttgaggcggt ccgcctttcc aaccaggtcc agcgcctccc
    10201 gggcccgccc ctgcttccac gccaccatgg cgcgcgtgac aagcaggtcg gttcggtcgg
    10261 gcgcggccgc caggccgagg gccaggattt cgtcggcccg cgcaaactcg cccatgcgcg
    10321 cgagcgtcat gccgagatta aacaggacgt cggcgttgcc ggggtgcaac cggagcgctt
    10381 cttcaaactc ggcccgcgcc tcgggcaggc ggccggtccg ggcgagcgcc acgccgcgca
    10441 gatggcggac cttggcatcg tcgggcagca ggcgggccgc cgcgtcgagg cactcgacgg
    10501 cctcctcggg ccgctggata ttcaggagca gtcctcccag cagcttgagc gtcggcgcat
    10561 ggcccgggcg ctggcggatg gccaggacca attcgctcat tgcgggctgg gtctgaccca
    10621 ggcgctcgaa ggccaggccg cggaaataat ggtaatccgc ctcctgcggc cggtggccgg
    10681 cccagttgac gccgagaacc aggtagagaa tcacggcggc cgcgaggccc ggcacggcct
    10741 cgcgcattcg ctgctcgcga aagagcttcc accactcgga cagcgcgtac gcggcgaaca
    10801 ggagcagaaa cggaacgatc ggcgcgcgga agcggccggc cacgaagaac ggcaggtacg
    10861 acgcgaacaa cgagagaaca aggagcaaga tcagggccac ggattccgcg gctccggcgc
    10921 gcgatccggc gggagccgcc ggccggcgac gccacagcca gaacccgacg agcgcgaggg
    10981 ccagcgcggc cgtgaaattg ccgggcagcc ggcgcaacaa cggcgagaaa tggcgctccc
    11041 aatggtcttc cttgttgttg ccgacctcca cgggccccca gaaaagaagc gttttcctcc
    11101 cggccagttc cagggcgcgg cgtggatacc gccacgcgaa ccgggccgcc gcccgcgtca
    11161 gcatcgacga gacctgggaa tgccgcaagg gccggccggc ctcgccttcc agcctcttca
    11221 cgagggcggg ccaatcgaac accgagcgga agggccgcag ttccggcggc tccacgaaga
    11281 tgccgttggc ggcgggattg ttgccgatgt acaggttgat cccaccgttc gagcagatca
    11341 gcactcggtc gtgaccccga cggtagttcc ggatcgtggc gggcaggatc gtggccagcg
    11401 cgacggcggc cagcagcccg aaatgaagcc accggatccg cttctccccg cgccggcgat
    11461 gcgtgatcca gagcatccac agcggcagcg cggcggcaaa gacaaggaca ttgggccgcg
    11521 tcaacgcgta caggcccagg cagaggccgg cggcagcagc catccagacg cgcggcgact
    11581 ggagccacga ggccagcagg aggaccatca gcagccccag cgtgaccagc aggaccggct
    11641 ccaggaattc cgcctcgaag tagatgaacg accagtagac ggccatgagc gcggcgaaca
    11701 ccaggccggc ggcggaatcg aacattttcc ggcccagccg gaacgccagc aggacgttca
    11761 ggatgcccag tcccatctgc aggattcgcg gcgccatata gcggccgccc aggatacggt
    11821 aggcggccgc caggaaatac gggtagcccg gcggccggaa gaacggcgtg ctcggaaccc
    11881 gcgggtcggg cgtttccggc ggcggggtcc aatcgccggt gaccagcgcc cgcgcccagt
    11941 agtcgtggaa ggccgcgtcg gaggccggca tcgcgaacga cgggtcgcgc cgggcctcgg
    12001 ccaggtagag gacgcgcagg accagcgcgg ccagcaggat gatccccagc caggcggcct
    12061 cgctccgcgc aaaacgtctt acgatttgca tcgccgcgca ttatagctta caaaaaggct
    12121 tgcacaatgg cattgaccga catgcgtatc ttcagacaac tcgttatcgt tctgctgccc
    12181 gcgctatggc tgggaggcct gccggccgag gccgcgacgc cgcgcgcgtt ccgcctgctc
    12241 tccgaatcca ataccgaggc cgtcgggctg acgccggccg gcacgctgac gtggagcaat
    12301 caactgcccg cgggccggtt ccaggtggag cagaatttca acctgtccac cgggcagtgg
    12361 cacagcttca tctccggccc ggccacggcg acgtggcaca gcctgaagct ctgggacttc
    12421 aacgcgccga cgggcatggt gtacatcccg gcgggggatt acgtgcgggg ggacggctac
    12481 gcgtgggacc tccgctcgtt acccgtacat acggcttggg tcagcgccat atacatggac
    12541 cgtcgtgaag ttacaaaggc cctgtgggat gaggttcggg attgggggct cacaaacggt
    12601 tttacggatt tgtcggtcgg cgttggcggg ggcaagcgcg tgatggtcac ggtgaccaat
    12661 gacggcgtgc cgaccaacat ctgggtcaac atcgaggccg gccctgaaca cccggtggtg
    12721 tacatcacct ggtatgatat ggccaagtgg agcaatgccc gctcactccg ggaatcactg
    12781 acgccgccct actacaccga cacggccctc acgacggtat accggaccgg gcaggtggac
    12841 ctggccacga atcaggtgaa ttgggaatgc accggatacc ggttgccgac tgaagccgaa
    12901 tgggagaaag gcgctcgagg cggtttgatc cagcatcact atccttggcc cagtacgggg
    12961 acctcactgc cggacgtcat cgacggaact cgggccaatt actgggaaag ccgcgatgtc
    13021 tttgatcagg gcaccacgcc ggcgggatac tacgacgggt cccaggtcgt cacgaatgaa
    13081 ctgggcgaaa tccagcccgt ccaggacatg gccaatgcct atggcctgta tgatatgtgc
    13141 gggaatgtct gggagtggtg ctgggattac tactcgttta cctcgtacac gtgggccgtg
    13201 cagaacacaa acctgcggga catccgcggc ccacctttcc acgatacggc acagccctat
    13261 cgcgtggacc ggggcggaag ttggcgaact tcgaccaacg cattgaacga tatgcgttgt
    13321 gtacaacgct ggtttgaaac ggccggatgg gcatcaccca gcctgcatcg cggcttccgc
    13381 accgtccggc ggcctgtgac tccatagcaa gcccatgcgc tcgatccggc agatcgcggg
    13441 gcgggcggcg ctggcgtcgc tggttcccct gacgctgttc ggcatcgcgg aactggccct
    13501 gcgcgtcctg gatctcggct acccgacgga gttcctgctc gaaaccacgg tccgcggccg
    13561 ggccgcgctg gtggacaatc ctttttacgg ctaccgcttt ttcacgcccg ccctggcgcg
    13621 caatcccgcg ccgatcctga cctcccgcga caagccggcg gacaccgtcc gcgtcgtggt
    13681 cctgggcgaa tctgccgcgc agggcgatcc gctgatgcag ttcggcctgc cgcgcatgct
    13741 ccaaaccgtc ctaaacgggg ccgccgcaac cggtcggttt gaggtcatca acgccgcgat
    13801 gaccgcgatc aattcgtccg tgatcgtgga cattgcgcgg gagaccactg cctgccggcc
    13861 cgacctgtac gtcctctaca tcggcaacaa cgaagtgatc ggcccctacg gccccggcac
    13921 cgtgttcacc gcgctgcccg gcctgacgcg gatggccccg tggcgcgtct ccctgaccgg
    13981 ctggcgcttg gcgttctttc tccgcgaggg gctgacctcc cttttcccgt cccgaccggg
    14041 ctcttcgaaa tggtccggcc tcgcgatgtt ctcggagcgc cccgtgccgt tcggcgatcg
    14101 cagcctgggg ccgatgtacg acctgttcga gcggaacatt cgggccatcg tgaattgcgc
    14161 ccgggacgcg ggcgcgcaag tcattctctc gacggtcgcc gtgaatctct cggattgccc
    14221 gccgttcggc ggcccggagg ccgcctcggc cttcgcgctg gcccgggaac aacaggatcg
    14281 cgcgggctac aaaaaggccc gggatctgga catctaccgc ttccgcaccg acggcatgtt
    14341 gaacgagcgt ctccggaagc tggccgggga actgcgcgtg ccgctggcgg acgcggaacg
    14401 ggccttcgat gacgccgacg ccgacgcgga ctttttcgtg gaccacgtgc atttcacctt
    14461 ctccgggaca caccggctgg cctcgaccat cgcgggggcc gtggccggcc tgtaccccgg
    14521 tcgtttcccg cccgccgcct ggcccgacga ggcccggtgc cgcggggaac tcttttacac
    14581 gccgtggagc gagcttcaga tggcgatgat catgctcgac cgacgacgcc aaccgccgtt
    14641 cgaccggcag ccggacaacg ccgcgtgcct gctccgcctc gaggacgagg cgaaacgcct
    14701 gtccgtccag atcgctcaaa ccgacccggc ggaactcgcg tcccgatacg aggcgctgaa
    14761 atcgaagaac ccggaagact ttttccttcc gtttgactgg ggggccatcc tgtccgaccg
    14821 gtcgcgatgg gcggatgcgg cggaggtcct gctcccggcg ctggaactcg tccccctgca
    14881 tttcgaggcc cgcaaactgc cggccctggc gctgtgccgc agcgggaaga cggaagaagc
    14941 ggccggcgtg ctcctgggtc ccgagaagcg gaggggctat ttcgtggccg actatgcgct
    15001 ggagatcatg ggcctgctgg cggcggaggg acaaaaggaa caggccgccg ccctgggccg
    15061 ggccctgctg gaaaaagacc cgcgcctgcc gtggcacgag tgtattgaag cgatgacaaa
    15121 acgcctggag gggcggcggg cggacgaatg aagatcacca ggaacgagtt gcttgcctcg
    15181 gccgcgctga cgctgctggt ttttttcctc tatgccccgg ccgttcggtt cgctttcgtc
    15241 gattacgacg acccgatcta catcaccgaa aacagctggg ttcaacaggg cctctccgcg
    15301 gagggattcc ggctttcgct gcgggaatgg gtgagcggca actggcatcc gctgacgatg
    15361 tggacgcaca tgctcgatgc agagctgttc ggactgaacc cggccggtca ccacgcacag
    15421 agtatcctct ggcacgcggc gaacaccgtg ttgctgttcc tgttgttgag ccggatcgcc
    15481 ggcggcttct ggcccggcct gttcatcgcc gcgctctggg ccgcgcatcc gctcaacgcg
    15541 gacagcgtcg cctggatctc cgagcgcaag aacttggtca gcaccttttt ctggctcgcg
    15601 acaatcggtc tgtacatccg atttacccgc cgcccctcgc cgcacgcgct gatggccgtg
    15661 atcgccggct tcgccctggc cctgatggcc aagcccatgc ccgtcacgct gccgttcacg
    15721 ctgctcctgc tcgatttctg gccgcttcgg cgggtcgagg ggatcggccg ttccgcctgg
    15781 ccgacctgga agaaactggc cctggagaaa ctgccgctct tcgtcatcgg cgccgtgttc
    15841 atcctgacca cgctgaagac ccaggtccta gcgggcgcca cgggcatgag cagtttcctc
    15901 tcccttcacg cccgcctggc ctggctgccg atcttctacc tggattacct gcgcctgttc
    15961 ttctggcccc gggacctgtg cgtgctctat cccgccccga ccgcggcccc gtcgatccca
    16021 gcgagcgcgg gcgcgctgct gatcctgctg ctgatcacgg cgggcgcgct ggtcctggcg
    16081 cggcggcggc cctggctggc cgcgggctgg ttctggttcc tcggcaccct ggcgccggtg
    16141 atcggactcg tgtccgtcgg cctgcaccgg atcgccgacc ggtacctgta tgtcccgatg
    16201 ctggggctcc tgctcatggt cgtgtggagg ctcttcgacg ccgcgagcaa acggtggcgc
    16261 ccggcggcct tcgcgctggc cgccgcggtc gtcctcgtcc tcgggctggt cacgcggcac
    16321 gatctcccgc actggcgcaa cagcgaggcg ctatttcgcc gggccctggc cgtgacgcgg
    16381 gacaattacg ccatgcacta caacctgggc cggcagctca cgcttcagcg gaaattcgac
    16441 gaggcgatcg ctgaattccg cgaggccatc cggctccggc ccgactacgc gctggcgcac
    16501 aacaatctcg gctggacgct ctgcctccgg gacgataacg aggaggccct tccgtcgttc
    16561 cgggaatccc tgcggctgat gccggacaac caccaggcgc gtttcaacct cggtctagcc
    16621 ctgatgcgcc tgggacgcta cgccgaggcg cgcgagcagc ttgaaatcct gctccagcag
    16681 gcgccggctt atccggaagc gcaggatgtg taccagcagt tgttgaaggc cggccgttcg
    16741 taatcgcgcc gcccaccgat cacaacccca ccttcccggc ttcactcccg caccgggatt
    16801 cccttccgga atatctccgc gtggacgcga atccgttccg ccaggtctcc ggcctgcttc
    16861 tcgcgagcca gcgccagagc cctttcggcc gtcgcgatgg ctgcatcgaa gcgcccggtc
    16921 tcggccagcg ccgcggcgag cgtatcgaga tgggcgggat catggggctg aagccgcacg
    16981 gcctcctccg ccgactgcac ggcgcgggcc ccatcgcgca cggccggatc ctccgcggtg
    17041 gccagcagcc aggccagcgc atcccaggcc ttgggttgtt gcggatcgcg ctgcaccgcc
    17101 tggtcgagac tctcccgggc ctccgcgtaa cgcttcaggc ggctcaacac gtagccgcgg
    17161 aaggccagcg catgggtggt cggctccatg gatacggcct ggtccaggtc tgccagcgcc
    17221 gcctcgggac gggcctgctg aaccaggatg gcggcccggc ttaggcggca cttgacgtag
    17281 tgcggcatga tttccaacgc ccggtcataa tcggccagcg ctccggccgg atcccccgca
    17341 agaaaacgcg cgacaccccg gcagtagtnn nnnnnnnngc attgatgggc caacgggcaa
    17401 tcgagtcggt atacagcgca acttccgacc ggtatgccgc gttgcggtgg cggctggcga
    17461 cggcgaggga aagagccgcc acgcagagca ggacgggcgc cgcggccgtc ggccatttcc
    17521 agaagcgcga cagattccgc aggcccatga cgatcagggc cgccgcacaa gccaggggaa
    17581 catacatgcg atgctcggca atggtctggc cgatggccgg gaaaaaaacc gacggggcca
    17641 ggaaagaaaa gaagcacagg cccgcgaagc cccacggcgg ctgacggacc agcgcccagc
    17701 ccgtggccgc caggagaagc aggagtccca gcgcgggaat ccacacctcc cccagcgacc
    17761 ggatcaagat gcttccgtaa tcgaagacaa ggggatgggg ccagagcacc agtcgaatca
    17821 tgcgattcaa gccccaggcc tgcgtaaaca gataatccca ccacgtatcc gcgcgcaacc
    17881 cggcgtgcgc cagatggggg gaatagacca cgtggcgata gacgggccat agccccgtcg
    17941 cagccagggc cgcgaaatac ggaacgcgcc cgcgaagcaa ctccttccat gagggcgcca
    18001 ggaagatccg gccgtagatc agcgccagga ccggcgccac gatcatgctt tccttgctgc
    18061 ccatgccgag caggcaggcg aagacggaga gcgccagcca gaggatcgcg accttcggct
    18121 cgcggggacg ctcgccctcg cagaccttgt ccatactcat ggaaggccag cttcccgcga
    18181 gccgcatccc atgggcccgg tcgaggccat agagcatcag gaagaagaaa agcccgaccg
    18241 ccagttcgcc gcgctgcgag gggtagttga cggccgccgt cgccagggga tgcacggccc
    18301 aaatcagcgc gcaaaagaaa ctccattgcg ccgcttcggc atcggcgaac cccaggcggc
    18361 gaaaggcccg gctcaacagc ccgaacaggg ccatgacagc cgccaggtga aaggccatgt
    18421 tggtcaggtg gtatccgcgc ggattcaagg cccccaaggc atagttcgcg cacatggtca
    18481 ggttgatgaa aggccgggtg cagaacgtgc actccgccgg cggcggattc aatgcttcgg
    18541 acaacgattc caggcggcgg atggcgggat tgcctacaat cgagtcctga tcatccagca
    18601 caaaaggtcc gtccagcgtg ttctggtaca ccagcccgac agtcaacgcg aggagcaagg
    18661 cataacccgc cttgagcaga gatgatttca tgccggagcc cacagtagcg gaaaatgaag
    18721 aaaagttcca acaaccaaca gccgcggcat ctcgttgcgg ctacagttga ttgacgaaga
    18781 aaagaagcgg gcccgggagg acccgcttcc ttcgttctgc ggatgttacg aactagcgac
    18841 gacggcgata ggccagcagc ccggccacgc cgaggcccac gaggaccagc gtcgtcggct
    18901 ccgggatact ctggatgaag aacgtggcgc tgtagttttc gccgctgaca ccggacagcc
    18961 aactgtcccc ctgccccgtg ccccagctct tcgcccacac cgtcagggtg tgcgtccccg
    19021 ggctgccgcc ctgcaccacc acgtgggtcg ccggctcggt gccctggtag tcgttgccgc
    19081 cgaggtaggt ctgggtccag aggatttcct ggtagcccgt gccgtcgagg gtgtagtaga
    19141 acgccccgcc gtcacgatcc gccgtatcgc tccagtaccg catgtccgcg ccgatacgaa
    19201 ccgagttggc cgcgcccaca ttgccgagga aggcgccgtt gaactgcgtg ccgaatgtag
    19261 gatcgccgcc tatccagtag tagcccgcgg gatcgccgtt gaggctgatc aggacataat
    19321 cctgatacca gcccgtggcg cccatcgcgg atagagcggc cgccaaggcc acaagaccga
    19381 taatgatttt gcgcataatg gtgttctctg tgaaaaaaaa gaagcgggcc cgtgaaggcc
    19441 cgcttctttt caaccgtcat gccgtcttag cgacgacgac gataggccaa cagaccggcc
    19501 acgccgaggc ccacgaggac cagcgtcgtc ggctccggga tcgccgtgat gccgaacgtg
    19561 gcgacatagt tttcgccggt gacgcccgtc agccagctgt caccctggcc cgtgccccag
    19621 ctcttcgccc acacggtcag cgtgtgcgtg ccgacagcca agcccgccgc gacgttgtcg
    19681 ctgctcgccg cgaggccctg atagtcattg ccgccgaggt aggtctgggt ccagatgact
    19741 tcctggaagc ccgtgccgtc gatcgtgcga tagtacgcgc cgccatcgcg gtccgccgta
    19801 tcgctccagt accgcatatc cgcgccgagt tccaccatct gaccctgaac aacgctgccc
    19861 aggttcgcgc cattgaactg cgtgccgaac gtcggatcga ggccgatcca gtagtatcca
    19921 tccgcgccgc cattaaggct gacgaggacg taatcactgt accagcccaa ggcgcccatc
    19981 gcggacaccg cggccatcaa ggccaccaac gctacaatca gtttacgcat ttctctgtca
    20041 ctccttttac gggtttgcct gcacttaagt gaatgaccac aacagccact cacaagtcgg
    20101 taaatcgttt aacaaagaga ggtgcacctt ggcaacaact atttttaaat aaagtgcatt
    20161 ttcaagtttt ccttcgaaat tcatgctgtt ttttaatctg aacggcccgc tgtgtgggcg
    20221 tcaggatgcc ctttcggagg caggctgacc agggcaacta tttttttcca cctgaacaaa
    20281 gcgggcacgc cttaaggatt ggctccatcc cgccgcccat tgtacagtgt gccgtcatat
    20341 gaacgcccag ccaatccgct cgatcacgct gctcggctcc tcgtccggcc ggaacgccgg
    20401 cgatgccgcg ctgatcgccg ccatcatgga caacgtggac cgcgccctgg gacgccgggt
    20461 ccgctacgag attcccacgc cgcgcccgga ctacctccgc catcactacc cgaatgacgt
    20521 tcatccggtc ggcatgatgc cgtggcagtt gtcggtcaag atgctgggcc tgccgacgct
    20581 gcgctccatc ctgcgcacgg acctgacgct ggtcttcgac gccatcctgt tcgaccgctc
    20641 tctctacaac ccgctcttca acttcctctc gacgctctac cttctcctcc cgctggcccg
    20701 gaagttgggc aagcgcatgg gctgctacaa cgtcagcgtc gggcccatcc ggacccgggc
    20761 cggcgcacgc atgctccggc gcgtgctcgg ctgcatggac ttcgtcgccg tccgggacac
    20821 ggaagccctc gacctgttcc gcgagatcgg gctcgagcat ccccgcgtgc gcctgaccgc
    20881 ggacgcggcg atcggcgccc ccgcagccga ccgggcgcgg gtggatgaga ttttccgaaa
    20941 ggccgggctg actcccggcg agcccgtgct gggcatcaac atcaaccgct acctggacac
    21001 gtgggcctcc aggagccggc cccccatggg cgcggaggcc ttcctcggga cgtacgcggg
    21061 ggcgctccgc cgggtctggc gggaactgcg cgtgccgctg gtcttcgcgt gcacccagca
    21121 tcatgacgtc gagatcacgc aggagctgat gcggcgcctg gaacccgatg tccgcgcggc
    21181 cttgatcgcc aaccgcgact atgatcacgc cgagaccaag ggcgcgctgg gccggctgga
    21241 cctgctgtgc ggaatgcggc tgcactcgct gatcctggcc tcctccgggc tggcgcccat
    21301 catcgggatc gcctaccagc cgaaggtgaa ttactacttc aacaccctgg gcctgggcga
    21361 cttcgtgttg ggcttcgacg agttcaccga ggagaaactg gccgcccata tcctccgggg
    21421 ctggcgcgag cgcgcccggc tccgcgcgac gctggaacag cgcatcccgg cgatgaccct
    21481 ccaagccgcc cgaacggccg acctggtcgc ggggatggag agaggggttt aggctgtagg
    21541 ggggcttagg ctgtaggggt gatgagatga gggatcatac caagctgcgt gcttttgaac
    21601 tggccgacca ggtggtcatg ctgacctatc aaataacccg ccatttcccg aaggcggaag
    21661 cgtacggatt gaccgcccaa atgagaaggg ccgctgtttc cattgcctcg aacatcgtgg
    21721 aaggatgcgc gagagggagt caaccggatt accttcgttt ccttgaaatc gcattcggct
    21781 cgctgcgaga gcttcactat caacttactg ttgcgcgacg cttgggatac tgcgacgagg
    21841 ccggcttcat cgaaggcgac acgatcatgc tggaagccga aaaagtgctc ggggcattga
    21901 tccgtgcact acgaaggaat tgacaaacct acagccgacg cccctacagc ctaaaaccct
    21961 cccgtgtcct cctcttccac gcgactgttc atcgtcacct tgatcctaac cgccgccgtg
    22021 tggctttcct tttcctggcc gctcgcgctg cacctccgcg acggaatccc gatgtcgtcg
    22081 cagaatgtcg agaaaggcca cgcgcgggcg atggtcccgg gggatcatct tcaactcctt
    22141 taccacttcg aactgctccg cgacatgctc gcggggcgcg tcccgtggtt ccataacgtc
    22201 tacgaattca acacgggcga cgacgcggag cgctaccggc ccagcgccta cttcgcgccg
    22261 ttttcgcttc cctatgccct gctggccgcc gccgggggcc gcgcgctcgc gtggaacctg
    22321 gtcgggttcc tgtcgctgtg gctgaccgcc ctggccacgg cgctgctcgc cgggcgtttc
    22381 gcgaccagcc ggctggccgt gtacggggcc gccctggccg ccctgcttat tccattccgg
    22441 tgggtcaacc tgctgggcgg cagcccggcg ggactcgcca tggcctgggt gccgctcacg
    22501 gcgctcggcg tggacctggc ggtgcgcgac cgccggatgg gcggcgggct cctcgcgggc
    22561 ctggccatcc tcctggcgtg ctggagcgac acgcaggtgt ttttcttctg cacgatcagc
    22621 ctcccgctgt ggctgctgct ttcctggatc ggcagcgcgg aaaccgggac gcgcgacctg
    22681 aaacgcgtcg cggcggcgct ggcccccatc gcgctgtttc tcctggccgc attcttctac
    22741 cggtatctcc ttcacgagca cctgtcctct tccggcatgg gccgcggccg cagcctggcc
    22801 gaggtgaagc tgttctcgcc cacgtggccg ggcttctggc agtgggcggc ggaaggattg
    22861 cagagccaca tctacatcgg ctggatgctt ccgctcctgc tgctggccgg ggggtacgct
    22921 ctccggcgga acgtccggac gggcgcgccc ggaagctttt ccacgttttt tgtcttcctg
    22981 ctctgcgcgg cggcgatcgg ggacgtgatg atcctggcgc tgggcgtaca cggaccggtc
    23041 gacggcctgg cgctcaagat cgtgcgcaag ctgctcccgc cctactccat ggtgcgccag
    23101 acggccaaga tcttctgcct ggtcccgacg ctggcggcgt tggccctggc gctgggtctc
    23161 gagggctggc tggcgttcgc gaccgcgcgc ccccgtctcg cgcggggcgc ggcgatcctc
    23221 gggctcctgc tgctggcggc ggagatgcgc ggacaagtgc gcccgacggt atgcctgctg
    23281 gattccgggc agggcgccta tgccgccgtg gccgaagacg cgcgcggcgc cggcaacccg
    23341 cccggcgcgc tggtgctgcc cctctggccg ggcgattcgt cgtgggcgtc catctacgag
    23401 tattacgcgc agatgcacgg gatccggatg atcaacggct acagccccgt cgtacgccgg
    23461 gattacgtgg agcaggtctt ccgcgccctg gaaagcgcca accagggctg gctgaccgac
    23521 gagcaactgg acctgctggc ccggatgaaa accggttacg tgctggtgca cgagaacgcg
    23581 ttccccgaga aagtgagccc gtttccggtc ggcctcacgc tggatcgatt gcggcggcac
    23641 ccgcgcctgt cgttcctggg ccaggcagaa aacgtgtggg ccttccgcat cgagccggcg
    23701 gccgcccccc gcgcccccgc cccgcccggc cccggctgcc cggtcgcctt cccggcccgc
    23761 cgatgggaat gggaatccgt cgaatccggc aacgggacgg ccatcgagga tgcgtccgcg
    23821 ggcggcgggg cgttctggcg ggcgtccgac ccgggagcct gggcgggcac ccgtcccgtg
    23881 cgcgcggcgc ccgatcccgc cctgtactgg cttgtccgcg gccgcggccg tggcgcggtt
    23941 gaggcgatca cgttgagcgg cgaggcggca ctccccgcgt cccgcctcgc gttcgattcc
    24001 actgaatgga cctggcaatc gatccccgtg tccgggcacg acacctacgg accgcttcaa
    24061 ctccggttcg cccgcgcgga aggctccgtg gatctggatg tggcgatgct ctgttcggga
    24121 agctggccgc aggcctggcc acccggcgat cgcgtggaga tcccggcggc ctgcctgttc
    24181 cacggcgggc acagcggggg agacgaagtg atcttccgcg cccggcacga cccggagtcc
    24241 gcgattctct acggccccaa gctgccgctg ccgcccggtt tgtaccgcat ggaactcgtg
    24301 ttttcctccg gcgccccggc cggcgagccg ctcggccggt tcaacgtccg gacgcgggag
    24361 gaggatgagt ccggcgccgt aagggtgacc gccgggcggc cttgcgtaac aacttggcga
    24421 caaacaggaa gcttgcctgt gaatgccgtc tttgtatatg ctcgaaacca cgatgttaag
    24481 gtggtcggtc tgatcatcga ggcggtccat gctgcgcaag aaggattggc tttaccgggt
    24541 ctatagcgtc ggttccgttt tctggttgct cacctaccaa tccattccgg gctgctcgga
    24601 cacgggaccc agcggcccgc cgccggtcgg cgcggacatc catgggaaat ggaaaggcga
    24661 gttctaccgg accgaccgcg gctatcagat cgaggtgacc gcccgggtgg aacaggagtc
    24721 cgatgccgtc atcatcgaca ccagcaagac cgaaggcgtc gcgcaactgc tgacgggctc
    24781 catcagctcc aacggctacc tgcgcctgac cgacgccaac accggcgaga aatggaccag
    24841 ctacttcggc ccggccagcg agacgaacct agtggtggcc gactacgccg agacccctac
    24901 catcgaacat cccgagcccg gcctgtacgt gctcgcgctg caccggtgag cggcccgcca
    24961 tgacctgcga gcgccattct tgaaacggga cctagccagg ctttcggacg aatcgttcga
    25021 ccttgtcatc atcgggggcg gcgtgtacgg cctgtcggcg gcctgggacg ccgccctgcg
    25081 cggcctgcgg gtggccgtgt tggaaaaggg cgacttcggg gcggcgacgt cgtccggctc
    25141 gctgaagctg atccacggcg gcctgcgcta cctgcagcac ctgaatttcg cccgcatgcg
    25201 catctccatc aacgagcggc atcgcatgct gcgcatcgcg ccgcacctgg tccacccgct
    25261 ggaattcgtc atgccctgct acgggcatgc catgaagggg cccgaggcga tgcgcctggc
    25321 cctgctcttc aacgacctga tgtcctttga ccgcaatcgc ggcgtggacc gggcgcacgc
    25381 gataccgaac ggccgcacgc tgtcgccggc cgaatgcctc gaccggctgc cgggcatacg
    25441 gcgggaaggc ctgaccggcg gggcggtgtt ctacgacgcc cagatgcaca actccgagcg
    25501 cctgactctg tcgttcgggc tttccgccgc ggagcagggc gccgcgctgg ccaactacgc
    25561 ggaggtcacg ggatttcatg tcgagcagga ctccatccgc tcggtcatcg cgcgcgaccg
    25621 gttgagcgac cggatgttcc atgtcgccgg ccgcctgttc ctgaacctga cgggaccgtg
    25681 ggcggacatc acctctcgcc tgctgacctc gccgtccccc gaccgccgcg tcgtccggtc
    25741 caagggcatc caactcgtca cgcgcgcgct gccgccggcc gccgcgttcc cgatcgagag
    25801 ccggcagcgc gacctgaccg ccgtcatcaa gcgcggcggc cgcaactact tcgtcacgcc
    25861 gtggcggggc ctgtccattc tcggcaccac ggacacgctg tacgagggct ccccggacga
    25921 gttcgcgatc accgaggccg acatcgggga attcctggcg gagatcaatg cgctctaccc
    25981 ggccggcctg gagcgtaagg atgtccgatt ctggatcggc ggcatgcggc cgctgggcga
    26041 cgaggacacg aatcccgaac atgccaaggc ctcccacaag tcgcagatcc gcgaccacgc
    26101 ccgcgagggc gggccgtcga acctcatcag cgccgtcggg gtgaagtaca cggtctgccg
    26161 gcacattgcc gagaacctcg tggacctcgc cgtccgccgg ctgggcgcgg ccgccggccg
    26221 ctgccgcacg cgggagaccc cgctggccgg gggcgacacc ggggatttcg agtccttcct
    26281 agccgagtcg cggcgcgtcg cgccggccgg aacttccgag caccttgccc ggaactacgg
    26341 cactcggctc cggagcgtcc tggccctggt ctccggggat ccttccctgg cgcggccact
    26401 ggcggactcc acggaggtcc tgcgcgccga ggtggtccat gcggtccggg aggaaatggc
    26461 gatgcgcctg gacgacgtgg tcctgcggcg gacgggcctg ggcacgctcg gccatcccgg
    26521 gcgccccacg ctggaggaat gcgcccgcct catggcggcg gagctcggct ggtcccccgc
    26581 ccgccaggcg gacgaactcg cggccgtcga gcgggtgttc accccgcccg ccggctgaag
    26641 ggcgcgaccg cccgaccgtt caggtgaagc gctgctgcgg gttggggtgc tgcagcgagc
    26701 ggcacgaacc atcgttcatc agcacccgca ggtacatgtt cgcgggatac aactcgacgg
    26761 gcttggtgcc gtagtaacgg ggccgcccat cctcctcgtc cgggaactcc cgccagaatt
    26821 cgtcgaagaa ccgcccgtcg ctggtaaaca cggtgacccg gctgatctga tcgcggaagc
    26881 tcgcggggaa aacgaatttc agcgtctcgt gcatgttgtc cggattgctg acgaacccgc
    26941 cggccccgtc gttcagaggc agcgtctcgc agaaggtgtc gacgaacaac tcgccctggt
    27001 actcgtaggc cgcgatgccg agttcctcct ggccggcggc cgccgcgggc tcttccgggt
    27061 ttcctgccgc cgcgggctcc tccgagccgg ccgcggcggc ggacgc
//
LOCUS       DAOOTK010000033        26577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1285_length_26577_cov_5.887904, whole genome shotgun sequence.
ACCESSION   DAOOTK010000033 DAOOTK010000000
VERSION     DAOOTK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 26577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26577
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_26577_cov_5.887904"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK634_05775"
     CDS             complement(<1..185)
                     /locus_tag="PK634_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97279.1"
                     /translation="MLSTPALIKVLRIPAAYPALYFFLPQRQRNAANKAGDGGVKKKV
                     VVLSGRRSKVLPTIAGP"
     gene            311..3061
                     /locus_tag="PK634_05780"
     CDS             311..3061
                     /locus_tag="PK634_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97280.1"
                     /translation="MTRHLVGRMILALLLASAARAQVAPTPSNGIPTGDYSAGFEKFY
                     QMGLPNVRGAKYVRLNVFSGALAQGASRVYELDLGGNAWLLGEENGRGRFVMNPGRAC
                     DVLEYKLLRGRKAGGAGPAAGVWQDADLARDVAKTVSFLEQKIEDRKENDWAFRQNGY
                     GYLLLLAIHYHAQGYTREAHRICDLLFEHAGDFRMVLVQALNVLADLQYEESCDRFAE
                     SGDWAAYRADLEALLARYPAGWQTRPAVSRVAEKVQARIEKPAPPPLAGEDLTDEDRR
                     LAEELAEAALVPMVFRDLLVLPDSAPGGKEENAAHPLARIRARGLAAVPLLLALLKDD
                     YLTRMDTWSLGDGNSRMYIEFSSGGLPDELADRLYESLRRPLARGELAARLLQPLLPT
                     AASAEYGSTDREALYEDAKAWYAEHRNQSPVELARYYLAEGQDAQQRAAIQYLLAHGA
                     EEDAQALEKRLLEQAGDNWMRDMNLVTLYVTRRGPAARGFVDQYEAALRAGTGEETTL
                     TGQPAMDEQVDRLLKNLRQLASGETLEEFVRKIASGNEPYEAWLSLLWQRVSQEKPNV
                     ALAALLKAVPQASDARTAGELLGLARILRGGFGRREGDEAGEPLRMADHADLWRAVLA
                     DRRSIEDPGELSGATLGDQAAYAVEMLYGEQPATPREGTSYYNLGNRISDLMQRRAEA
                     RLEGKTGAELPELPSADRVTAERRSELSAIFGMGGPQDVPRKLSGLNNDELLALAELA
                     EKDSDLNAKLRPASLLIRDARFLGLADEETASWKAAIGKPLDADLARRALEETRSAVA
                     AGLPHTVSFTRRPILDGVVIVIEHGDAARSGMRMRADVTSAVMGALHLDDGGAGARWP
                     VGGSDAEADEGGESDDQDFWAALEHMLHDGEHVCAAGQLIFTGLPAERSDPDGASGTD
                     EE"
     gene            3087..4070
                     /locus_tag="PK634_05785"
     CDS             3087..4070
                     /locus_tag="PK634_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOW97281.1"
                     /translation="MADIGQDKWKKAQEAVALLRQGVAQVIVGQQQIVEEMLVALLCR
                     GHCLIVGVPGLAKTLLVSTLGQLLGLAFHRIQFTPDLMPMDIIGSEILQEDASGSRRK
                     FEFTAGPIFANLILADEINRTPPKTQAALLEAMQEKQVTVAGRTMPLAEPFIVYATQN
                     PIEHEGTYPLPEAQLDRFFFNLLIDYPTLDEERQIVRQTTGRQRPAVSKALDAAQVLE
                     LQDAVLDVPVPDHVLEYILKLVTASRPSREPADPFVRQYVEWGAGPRASQTLTLAAKA
                     LALLRGQPAASVEEVRHVAKPVLRHRVIPNYNATGEGISVERIVDHLLETV"
     gene            4070..4945
                     /locus_tag="PK634_05790"
     CDS             4070..4945
                     /locus_tag="PK634_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOW97282.1"
                     /translation="MGALRFKYLKPEDIRKLAGYEFAPRALVEGYLAGRHRSRARGSS
                     IEFRDYRPYVPGDDPALVDWRVYGRTDRHYLRTYEQETDMECHVFLDSSASMGFGRAI
                     PKLEYASFFTAALCYLVVRHNDRVSLQIFDEGIRRFFPPGSTGRHLRNLMAALEENRP
                     GNPTSVAAALRRSFPLLKRRGTLIVVSDFFDDPAAIFAALNAYLHRGFKVHLFHVLAP
                     EELELERRGLLHFVDLEDGRRVIAHTDSLARPYGEAMRAHIASLRELSVRRKVDYVLA
                     RTDTHYFNLFDRLTT"
     gene            4942..6957
                     /locus_tag="PK634_05795"
     CDS             4942..6957
                     /locus_tag="PK634_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOW97283.1"
                     /translation="MSFVFLNAILWPLAALAAVPLLLHLFARSKPPVYRFSSVEFILR
                     IVRTTLRVKRPQDWLLLLLRTALVVTLVLLFLRPLYFASRALAGPLGRKNVVVIVDAT
                     GSMGCNEGGRTRFATACAEASEVLAGLTSADRANVVWLDAEPDAVFPEMGVNMGYLRA
                     ALSRAELRPQTGDIAGALRLAVSMLENQDGRREICLVSDFQRAAWEKASLAVPDSIRL
                     VNLCVAQGDGENGALTDLVVDPPAPLKGEEVTIHAEVANYSARPRQQTVFLELGEGRY
                     NQDLMIPPWEKAGVSFRHRFRQPGIHTATVSLNEDRFTADDRRWAVIQVADSLSVGVS
                     GTNTAEGRFWLRALGALSWVSAEPIDPARLPKEAAYDAVFLADGNGADADRFADMLGR
                     GCAMVCVPSRGTPVGPLLAAAGVDASRVPAEAAWEILSGPTAARVARKEDRLFQLFAG
                     GEYGDPAGGVFRARWRLPGDAAAAGETLIAYEDGLPALVRYRHGGLLYLWALPLDPEV
                     SDWAARVEFVPFLGELLLSSRSARGGGATAAMPGDRLAWRVDREILASDVTLQHGAGE
                     ALPVLAQREAEGTRVVSESAADTGVYTWKYEGEAQQVTAVNFPALESDLRPIPVKEIE
                     QQGRVALAAGRRARELRDGLPLWPYLLLAVVGWALLEGGVQVWAERT"
     gene            6954..9164
                     /locus_tag="PK634_05800"
     CDS             6954..9164
                     /locus_tag="PK634_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97284.1"
                     /translation="MNPLLPIEDLALWAAVLAAGAGFLAWRSSAKCRPRIRLLLAGLR
                     VAGMACLAVIALNPGRWSDLSRRNPSELAILLDRSASMATRDAGEATRWDAASALVRD
                     VRRRAGGPEHTPLYVFADDVEPAGVRDPGDFKADGGATDIRRAVEGVLNLSRSGGRNL
                     AGILLLSDGRQVGGAASGDAALRARARDAPVFPLALGSAIERKDLSIEPLRRHHVTFK
                     GQRTRLVARVRASGLGPIRPAVRLLDGDGAVLGEETLSLEGDGEATVSFAVTPEGRGH
                     QEYRFETAGWEGESTRANNEARVGVAVLDGRIRVLMVEGVPYWDSKFIAQLLRRQPNI
                     EVISVYRLSADRFFRVETDLADAAQITGSVFPDSAAELDLYDLVVFGKGAEYFLTPER
                     IRLLRDFVRDRGGGLLFARGKAYNGEFPELEALEPMEWGERVASPFSVRPTDAGSAAG
                     LFGDMLPGPEDAVWGRLPPLQFAQRVRKLKAFSDVLVEGHSELAGHEQSFPVVVRRRF
                     GKGMVVVVNADGLWQWDFFPSVKDASNMYQEFWSQLVQWSATFSEFLPGSRYALGLSA
                     RVAHPGEPVRVRVTQRLPEPGAPAPEVRVQQGAEPARTLWPAPVPEEPGRWEAVFSLD
                     RPGSYQVALRAPGEQDAAAGVSDTLEVLAAPREEDNVSADPDFLRELAALSGGEVVTP
                     ETLDVVVRRLKEGEEDVKSGRATWEPAWDRAWFLVFTAFCFAAEWFVRRRNGLL"
     gene            9161..11938
                     /locus_tag="PK634_05805"
     CDS             9161..11938
                     /locus_tag="PK634_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97285.1"
                     /translation="MTAHDKPTELGRLLRQRLRQWQAGLAQLALLQALSRAILVLLGY
                     FVLDYFLALGSAARFALGALLLGLLAAVLAARGIRIAALTPRDMAVRADRRIGGRRRA
                     VLGALELETWLAGGAAREAGLMAYLAGQAVGRAADQVRRLDLRDALLEEEFCRHRRVL
                     YACAAAAALVFLVNVPATRTILARIFLPGRDVPPYSRYTFQVEPASPVVLYGGDLAVR
                     VEIGGRTVGGEAGFLTRAGGRIHRAACFQESATRYAQRLEGVIEPVEFCFTVGRARSR
                     WCPVDVLFDPHIAAAEVVLVPPAYSGKPARRFYLGAEDVSGLKGTRVELTLTSNRPLR
                     DGVIHWRFENDNDSAPALSGEKVGDHTVRFTWILRATATAEITVRDIRGTPNASPFKM
                     AQRVVPDEPPSVALSEPPPFSLATPGITVPVRGAADDDLGLRQVDLARALVGYRDRLQ
                     TVGPPEPASGFEYEGELALPKLGVEAGQVLELYLEAMDSNPSLAGISVSEVARIQIIS
                     EEEYALMLRVRISAEEFAGRFNEVHRDFTNLQEALRALSDELGKSERDADAVERAREE
                     ARRAAGRAAKAFDEVARDFPAFDMERRLQEAAAGMADRMRETEALLAGSPPPHDGLQE
                     QVDAFLAEYAAGRRALDEQVRDAGEAALLAEIMESAAHYARLLAEQEALVRRLSRFEG
                     GGRKEDLPILADLESRQADIRRQLTELLDRIDAQAGRLRLDYQQLTADMEAFVEKARE
                     LEIVPTMEDAELKARNHDGAETCRLAGRALEKMKELAGPEKGNCFGGMCQGQLRFDVR
                     QNMRSTLEQMLAAITMRICSGEGSMPGMGRGGSGLGGGGDDGYWMGGQSPLDVAVYGP
                     ERRAYRSSPAGGSGAGRGKGPGDHRIGKQESDRVAPAASGPVAAESMPLENVPENYRE
                     SVKRYFSAEEP"
     gene            11941..12588
                     /locus_tag="PK634_05810"
     CDS             11941..12588
                     /locus_tag="PK634_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOW97286.1"
                     /translation="MKRILFVAGLAAGLAAAAPAKEGVVQCANLIYGGTHTSRCFSDE
                     FLSAVQRDTAIATERRFKSVKLASEDLFSYPFVIITGEAEFHFTAKEREHLKKYLESG
                     GFLLASAGCSNKDWDRAFRREMKTLFAEKGLRPIEMDHPLFRTVHAITELKLHHPAAA
                     ARLEGIEVDGKLVVVYSPHGLNDTAHTEGCCCCGGNEITNSLQVNVNILVYALLY"
     gene            12572..16993
                     /locus_tag="PK634_05815"
     CDS             12572..16993
                     /locus_tag="PK634_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOW97287.1"
                     /translation="MRCFTDPWRFGKVLGLAGLLAAARSPAAEPPPDAAPATGVVTLV
                     WFPDLNAALNRAEAEYRPVVTLFTSPGCGWCRRLKAETLDDPEVRKLLQHFALVEIDV
                     MEDPDSAQRYSLRGVPTLLFLASDGRVRGGVSGYVSAPELCTLLQKELNPEFLAGQDA
                     PYRDLLKALDARKVPDEMWPAILAGMGEAQKRTELRDRIFALKPFPSASLVERLKDAR
                     LAVRLGALEILEEMAGASRDFDPWQSPEGEANREPLRQWQEWASGATGAVARVYAVLT
                     PEEIRTSLLDLVSDHRERSLRAMRLLEHGGPGTADAVAAFLSGHPDLPAGARGRIREL
                     QYVLLLPELAGTDVRALAHRLVFGNLDTRLKAIQDLPKFRAAATIVLADALEDPEPLI
                     REAAVDSLARADPRRAMDILGRHVSVEKDNEVVFAILRVLGGLTGRKGLDILSSYLQS
                     ENEDLVVAALQSLGRLKTQGVSDKVGACLKDTRWRVRAAALEAAASLKANSLADQVMA
                     LLDDPDPFVRDSAIHTLAALGAKRAAGRLEKLFLENDELKGPIVSAFLQMELRLPVSF
                     QAALKDRPPNVLLSVIEAVVEDGEFKDLSLIAPLTDHPDLDVACAAIQPLAQKGMSHA
                     PYRQRLADILRAGQPEKVVTVLESIEIEESNLSPSYATAVDFDALGAEGGSGAGTNSL
                     TELFEAFKPGGAEPPAPAPAPAETGAAGGLLKALGFKTAAPARSAVSGPDSLADAVQP
                     YAGPEYEGRTRFAAALLLTSFGQTGQVAFLQQTMGDRTEDERQQVAQCVRPRMRDAFL
                     PLLDALLDDESSAVRVAAVSAAVERGGSRGLERMMAALLRPGGRLKPGEVYSHDIESV
                     LRRQEARPRIRECAMLLLQQSNAPPRQALGLVLLQKTWQPSDRTAVWPFLYAGDPWVR
                     RAAFHALGQNDKASFRKVLDCAVADSSEYVRAVVPKVYERGMSRWTHYFSLDEFEADQ
                     YYWSVQDNSARLQDMEVAALRTLAKDADPRIRIESLMALLSNRQGVDLAELVRAAEAV
                     PDRRAVADRLGRYLTDNSARLGPEFSVLLPFLDESEQNESQKEKVRKILEKNRPAGEL
                     PVVAALDRPIEAVIRDAQTAPVPEVAAPSVRVIFFRSYGCRDCERAEAMFPQLREVFP
                     GLQIDIHDIRKTRAMSLNEALCERFGVPDRIRLVTPALFTAAGYSIKEDITFARLGEL
                     LTRSAGADDAWSRVEEAELDQAAASIGSRYRDIRLGVIAAAGLLDGVNPCAFATIIFL
                     LSYLQVTRRTSRQIAQVGLAFIAGVFVAYYAMGLGLVEIVSRLGVLRRFSLVVNLLLA
                     AFTLLIAILSVRDGIRCLRGRLADITLQLPGFLKDRIHTVIREGARHARFVAAAFVAG
                     LVVSVLELACTGQVYAPTILFMLKTGEERFAAMGYLLAYNAAFVAPLFVVFALAVFGL
                     RSDALIRFMNRHAAGVKFATAGLFLLLFAVFVWSLRG"
     gene            complement(17005..18042)
                     /locus_tag="PK634_05820"
     CDS             complement(17005..18042)
                     /locus_tag="PK634_05820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOW97288.1"
                     /translation="MNSRAGIVCIIAGLTVLAGPARETAAITITNSGIVRLVLNFSDQ
                     LQERLRTAYLDQLGPYQDHLWPIGRSTTPDTPFSSPYGPRLKASESYRYDWHRGVDIP
                     APLFTPVYAIASGTVTKAGFTNSYSDALVQLKHRQADGSGYYYSNYQHITNWYVNVGD
                     SVTQGQWIADSGCSTSGFDHLHFEIRDSSSWQSNCVNPFLAMPYSNSGPPYVQIDSVD
                     SSTPSRPVVQVTVTTPAEELDLNTVKATVYRWSTNPAAYVVYDSHTFDMMYWNKLHTP
                     TGTTATATLDQSNFNGVVISPAVFSETSTEYSITLTFTNLTGPGYTTNVYVSVQAEDV
                     EGESDSDRCGL"
     gene            18297..20315
                     /gene="fusA"
                     /locus_tag="PK634_05825"
     CDS             18297..20315
                     /gene="fusA"
                     /locus_tag="PK634_05825"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOW97289.1"
                     /translation="MKELAIGNVRNVVLVGHTGAGKTSLLDAVLYKLGVNDRLGSPAE
                     GTSVADWSDEEKDRKITIWAKPFNAVYASAQGGKYDLVITDTPGYADFYGQTVAATAV
                     ADAGLVVVDAASGIQVGTNRSWRRCETLGLPRGIVITGLDKENTSFDDTLAGLRAVWG
                     DRCRPATVLGKDKKSVADVFTGGGEVESLKSALVEAAAETDDQLIEKYLGGETLAPAE
                     IADGLRKAVATGKLIPVFVTLAKTGAGIPELLEGLVRFFPSPADREFKDAEGAVVAAG
                     ADAPFVGQVWKAVNDPFVGQLTILRIFGGTLRSDSEIYNATKEQKERIGPIYLLNGKK
                     QESVAEAGAGEIVALAKLKATVINDVLCAPGKSAKLTPIQFPNPTAAYAVAPKTQGDE
                     DKLSTGLHRVADEDPTIRVERNTETKELILYGMGDLHLDTTLQRMKKRHNVEIALSTP
                     KVPYRETVTALGEGHYKHKKQTGGRGQYGEVYLRVQPMKSGETEWFEDALVGTNVPRN
                     FLPAIQKGCVEGMNRGALAGYPVTNTKVSIYDGSSHDVDSSEIAFKIAAARAFSDGMS
                     KARAVLLEPIVTAKIMVPDQYMGDITGDMNHKRGRILGIGTEDGMQVITAEVPQAEMF
                     RYSSELRSITGGRGSFEMEFTRYDIVPSNVAQKIIAEAAKNKKAAEEE"
     gene            20399..21151
                     /locus_tag="PK634_05830"
     CDS             20399..21151
                     /locus_tag="PK634_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOW97290.1"
                     /translation="MSGHSKWANIKHKKGAADAKRGKIFSKIAKEIMIVVGASGKDVA
                     SNPALRTLIQKARAVNMPADNIDRAIKRGTGELQGAKLEEIMFEGFAAGGVALIVQAL
                     TDNRNRTVAEVRHSFTLHGANLAGQGQVMRTFQRKGQILVDAAGVDEDKLMELVLEAG
                     AEDMRREEDQFEIITDPAQYPAVAEAINKAGITAASSEVTLLPLTWTPVTDKAKASSL
                     LAFIDELEELDDVQNVYSNFDIPAELLEALNK"
     gene            21170..21667
                     /gene="ruvC"
                     /locus_tag="PK634_05835"
     CDS             21170..21667
                     /gene="ruvC"
                     /locus_tag="PK634_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOW97291.1"
                     /translation="MSVLGIDASLRSTGYGVVARQGASFRCLDQGLVRNPPAHLHSEC
                     LAAIHARVTALLETHRPEAVALEGGFYFKNAKTALILGEVRGAVIAACALRGVPVYEY
                     APRRVKQGLTGAGAASKEQVGRMVASILGLTELPPEDAADALAIALCHFHGRTGIAAL
                     QPKPI"
     gene            21756..24386
                     /gene="clpB"
                     /locus_tag="PK634_05840"
     CDS             21756..24386
                     /gene="clpB"
                     /locus_tag="PK634_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOW97292.1"
                     /translation="MQLDRLTIKSQEALQRAQQLAGDYGHAEVDVDHLVLALIDQEDG
                     VVRPLLERLGIPAEQLREPLERSLSARARVEGGAERYLSRSLRQALDAAFRVAEKMKD
                     EYVSGEHLLLAALPQADSETARVAARLGLTPDAVLKALQAVRGNQRVTDPAPEDKYEA
                     LKKYGRDLTEAARKGKLDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVAGDVPEGLKNKQVVSLDLGSMIAGAKYRGEFEDRFKAFLKEVIGAEGRIILF
                     IDELHTLVGAGRAEGAVDASNMIKPPLARGELRCVGATTLDEYRKHIEKDAALERRFQ
                     PVQVNEPTVEDTIAILRGLRERYQVHHGVRIQDGALVAAARLSHRYISDRFLPDKAID
                     LVDEAASRLRMEIDSLPTEIDEVERRIMQLEIEREALRQEKDEASRERLAALEKELAE
                     QREKSRGMKLHWQQEKEHIAEIRKLTEALDLLRGEEEAAKRNGQLERAAEILYGRIPK
                     IEAQVKAAQQKLADLQKNQRMLKEEVDAEDIAAVVSNWTHIPVSRLMETEKAKLLHME
                     ERLRKRVVGQDEAVAAVSNAVRRSRSGLQDPNRPIGSFIFLGPTGVGKTELARALAEF
                     LFDDEHAMVRLDMSEFMEKHTVSRMIGAPPGYVGYEEGGYLTEHVRRKPYSVVLFDEI
                     EKAHPDVFNVLLQILDDGRLTDGQGRTVDFTNTVVIMTSNIGGPAIHEALQRKPDLKS
                     SDSAYEQLESRVYEELRAQFKPEFLNRVDEIVIFHSLGREQIKQIVEIQLARVRKNLE
                     ERHIAMDLTDAARDFLAEEGYDPSFGARPLKRAIQRRILDALATEILKGAVAEGDRVT
                     ADAARDGSGLAFRVAPRPGT"
     gene            24518..24979
                     /locus_tag="PK634_05845"
     CDS             24518..24979
                     /locus_tag="PK634_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOW97293.1"
                     /translation="MTVREIQVEVKGLVPTQSGVGVFLGHGDKVIAIFVDHSVAAAIT
                     MFMHKLKKPRPLTHDLIGSILAGLGVKAQKVVVNDLKDDTFFARLFLLQEGELGRNLV
                     EIDSRPSDAIAVALQQGCPIYVNTSVWERAEDMSWALKQNPPEAPSTEEHS"
     gene            complement(24999..26087)
                     /locus_tag="PK634_05850"
     CDS             complement(24999..26087)
                     /locus_tag="PK634_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOW97294.1"
                     /translation="MKIVDKYLFKAVLVPLLYCLAGFILIYIIYDLFDNLPDFIEGRT
                     PLLSVIRFYCFLMPSVLIIIAPVSLMLAVLYSLSHLTKNNELTAMRACGISLYRLMAP
                     LVILGLSASILVGVIHETLGPWSAYWAHQFIVMQKSKGDLSTHVVSPLPYRNELGRRD
                     WMIGAFNTQTFDMRGVNVIQQRPDGSEEYRIVAKSGHWYDGRWWLQDLSFQAYDAGSN
                     PLGPPRFEIHREMTDYNEVPSDFINEIKDPEYLSSLEILTFLKTHKRLSNETVSRVRA
                     NLHHRLSMPWNCLVVTLLGIPFGAKSGRHGAFLGIVLALSLFFGYYVFVNVGLAFGKK
                     QLIAPWIGGWLPNLAFLAVGLILIHRMR"
     gene            complement(26161..>26577)
                     /locus_tag="PK634_05855"
     CDS             complement(26161..>26577)
                     /locus_tag="PK634_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97295.1"
                     /translation="FSTFSLDFLQALESRLTAILSQAGQPLSLDELMKRAGTETLASL
                     REPAAAIECLLDHAPAVASTTDRRFFLYRNGAGPYVVEVLGRLDRPAHYRKITSTFNE
                     SLKPAKRKGAGYVLELLIATPRCTRVDRGIYDLKAE"
BASE COUNT         4250 a         8837 c         9501 g         3989 t
ORIGIN      
        1 caaggaccgg ctatcgttgg tagtactttc gagcggcgtc cagaaaggac gaccactttt
       61 tttttcaccc ctccatcgcc cgctttattc gcggcgttcc tctggcgctg cggtaaaaaa
      121 aaataaagtg ccggataggc ggccggaatg cgtagtacct tgatgagggc cggggtggac
      181 agcatccgcg ctttcactgg ggaaggtgat tgtattagta tgggcgcgtc acaccggggt
      241 gaatgaacgg acatccgcgc gaagcggtca taaccatgtg tttcaattcc gccagggagc
      301 gcgacatccc atgacccggc acctcgttgg ccggatgatc ctggcgctcc tgctggcctc
      361 cgccgcgcgg gctcaagtgg cgccgacgcc ttccaacggg atccccaccg gcgactacag
      421 cgcgggattc gagaagttct accagatggg gctgccgaat gtccgcggcg cgaagtacgt
      481 ccggttgaac gtcttcagcg gcgccctggc tcagggggct tcgcgggtat acgagctgga
      541 cctcggcggc aacgcctggc tgctgggcga ggagaacggc cgcgggcggt ttgtcatgaa
      601 cccgggccgc gcgtgcgacg tcctcgaata caagctgttg cgaggacgga aagcgggcgg
      661 ggccggtccc gccgccggcg tgtggcagga cgcggacctg gcccgcgacg tggcgaaaac
      721 cgtctccttc ctggagcaga agatcgagga tcggaaggag aacgactggg cgttccgcca
      781 gaacggctac ggctacctgc tcctgctcgc catccattac cacgcccagg gctatacgcg
      841 cgaagcccac cgtatctgcg acctgctgtt cgagcacgcc ggcgatttcc gcatggtcct
      901 ggtccaggcg ctgaacgtgc tcgcggacct ccagtacgag gagtcctgcg accgcttcgc
      961 cgagagcggc gactgggcgg cgtaccgggc ggacctggag gccctgctgg cgcgctatcc
     1021 ggcgggctgg cagacccggc ctgccgttag ccgcgtggcc gaaaaggtcc aggcgcgcat
     1081 cgagaagccg gcgccgcctc cgctggccgg cgaagacctg acggacgaag accgccgtct
     1141 ggcggaagag ttggccgagg ccgccctcgt gccgatggtt ttccgcgacc tgctggtgct
     1201 gccggactcc gcgcccggtg gcaaggagga gaacgcggcg cacccgctgg cccggatccg
     1261 ggcgcggggc ctggcggcgg tcccgctgct gctggccctc ctgaaggacg actatctcac
     1321 gcggatggac acgtggagcc tgggcgatgg gaactcgcgg atgtacatcg agttctcgtc
     1381 gggcgggctc ccggatgagc tggcggaccg cctctacgag agtctgcgcc gccccctggc
     1441 gcgcggcgag ctggccgcgc ggttgcttca gccgcttctc ccgacggccg cgagcgcgga
     1501 atacggttcg acggaccggg aggctttata cgaggacgcg aaggcctggt atgccgagca
     1561 ccggaaccaa tccccggttg aactggcccg ctactacctg gccgaaggcc aggatgcgca
     1621 gcagcgggcc gcgatccagt acctgctggc ccacggggcg gaggaagacg cacaggcgct
     1681 cgagaagcgc ctgctggagc aggcgggcga caactggatg cgcgacatga acctggtgac
     1741 cctctacgtc acgcggcgcg gtcccgccgc gcgcggattc gtggaccagt acgaggccgc
     1801 gttgcgagcc gggacgggcg aagagacgac gctgaccggg caacccgcta tggatgagca
     1861 ggtcgatcgc ctgctcaaga acctccggca actggcctcg ggcgagacgt tggaggagtt
     1921 tgtccggaag atcgcgagcg gaaacgaacc gtatgaggcg tggttgagcc tgctctggca
     1981 gcgcgtgtca caggagaaac cgaacgtggc gcttgccgcc ctgctcaagg ccgtgccgca
     2041 agcgtccgat gcccgcacgg cgggggaact gctgggcctg gcgcggatcc tgcgaggagg
     2101 cttcggccgg cgggaaggag atgaggccgg ggaaccgctc cgcatggccg atcatgccga
     2161 cctgtggcgg gcggtcctcg cggaccggag gtccatcgag gatcccggcg agttatccgg
     2221 cgcgacgctg ggcgaccagg ccgcgtacgc cgtggaaatg ctctatggcg agcagccggc
     2281 gactccgcgg gaaggaacct cgtattacaa tcttggaaac cggatcagcg acctgatgca
     2341 gcgtcgcgcc gaggcgcggc tggagggcaa aaccggggcc gagctgccgg aactcccatc
     2401 cgccgaccgc gtcaccgcgg aacgccggtc cgagctctcc gccatcttcg gcatgggcgg
     2461 gccgcaggat gtcccccgca agctctccgg gctgaacaac gacgagttgc tcgcgctggc
     2521 cgaactggcg gagaaggatt ccgacctcaa cgcgaagctg cggcccgcct cgctgcttat
     2581 ccgggacgcc cggttcctcg gcctggcgga tgaagagacg gcctcgtgga aggccgcgat
     2641 cgggaagccc ctggacgcgg acctcgcccg ccgcgcgctg gaggagaccc ggagcgcggt
     2701 ggcggccggc ctcccgcaca ccgtgagctt cacccgccgg ccgatcctgg acggcgtggt
     2761 catcgtcata gagcatgggg atgccgctcg gtccggcatg aggatgcgcg ccgacgtgac
     2821 ttcagcggtc atgggcgcgc tccacctgga tgacggcggg gccggcgcgc ggtggccggt
     2881 gggcggatcg gacgcggaag cggacgaggg cggggaaagc gacgaccagg atttctgggc
     2941 ggcgctggaa cacatgttgc atgacgggga gcatgtctgc gccgcgggcc agttgatctt
     3001 cacgggcctg cccgcggagc gaagcgaccc ggacggggcg tccgggacgg acgaagagta
     3061 aacgataacc aaccagcgag gatcgtatgg ccgacatcgg acaggacaag tggaagaagg
     3121 cgcaggaggc ggtggcgctg ctgcggcagg gcgtggcgca ggtcatcgtc ggccagcagc
     3181 agatcgtgga ggagatgctc gtggccctgc tgtgccgcgg gcactgcctg atcgtgggcg
     3241 tgccgggcct ggcgaagacc ctgctggtga gcacgctggg ccagctgctc ggcctcgcgt
     3301 tccaccgcat ccagttcacg ccggacctca tgccgatgga tatcatcggc agcgagatcc
     3361 ttcaggagga cgcgagcgga tcgcgccgga agttcgagtt cacggccggc ccgatcttcg
     3421 cgaacctgat cctggcggac gagatcaacc ggacgccgcc gaagacccag gccgcgctgc
     3481 tggaggcgat gcaggagaag caggtcaccg tcgccggccg caccatgccg ctggccgagc
     3541 cgttcatcgt gtacgccacg cagaacccga tcgagcacga gggcacatac ccgctgccgg
     3601 aggcgcagct ggaccggttc ttcttcaacc tgctgatcga ttacccgacg ctcgacgagg
     3661 agcgccagat cgtccggcag accaccggcc ggcagcggcc cgcggtgtcg aaggcgctcg
     3721 acgccgcgca ggtgctcgag ctccaggacg ccgtcctcga cgtgcccgtg ccggaccacg
     3781 tgctggagta catcctcaag ctggtcacgg cctcgcgccc gtcgcgcgag ccggccgacc
     3841 cgttcgtccg gcagtacgtc gagtgggggg cgggcccgcg cgcgtcgcag accctgacgc
     3901 tggcggccaa ggccctcgcg ctgctgcgcg gccagccggc ggcctcggtc gaggaggtgc
     3961 gccacgtggc caagcccgtg ctgcggcacc gcgtgatccc caactacaac gccacgggcg
     4021 aggggatcag cgtggagcgg atcgtggacc atctcctgga aaccgtctag tgggcgccct
     4081 gcgattcaaa tacttgaagc cggaggacat ccggaagctg gcgggctacg agttcgcccc
     4141 gcgcgcgctc gtcgagggct acctcgccgg gcgccaccgc tcccgggccc gcgggtcgtc
     4201 catcgagttc cgcgactacc ggccctacgt gcccggcgac gacccggccc tcgtggactg
     4261 gcgggtctac gggcggacgg accggcacta cctgcggacg tacgagcagg agacggacat
     4321 ggagtgccac gtcttcctcg acagcagcgc gtcgatgggc ttcggccgcg cgatccccaa
     4381 gctggagtac gcctcgttct tcacggccgc gctctgctac ctggtcgtgc ggcacaacga
     4441 ccgcgtgtcg ctgcagatct tcgacgaggg catccggcgc ttctttccgc ccggcagcac
     4501 cggccgccac ctgcgcaacc tgatggccgc gctcgaggag aaccggcccg ggaacccgac
     4561 gtcggtggcc gccgccctcc gccggtcctt cccgctgctc aagcggcgcg gcaccctgat
     4621 cgtggtctcc gacttcttcg acgatccggc ggcgatcttc gccgcgctga acgcctacct
     4681 gcaccgcggc ttcaaggtcc acctgttcca cgtcctggcg cccgaggaac tggaactgga
     4741 gcggcgcggg ctgctccact tcgtggacct cgaggacggg cgccgggtca tcgcgcacac
     4801 ggacagcctg gcgcggccct acggcgaggc catgcgcgcc cacatcgcca gcctccgcga
     4861 gctgtcggtg cgccggaagg tggactacgt cctggcgcgg accgacacgc attacttcaa
     4921 cctgttcgac cggctgacga catgagcttc gtgttcctca acgcgatcct gtggccgctg
     4981 gcggcgctgg cggcggtgcc gctgctgctg cacctgttcg cgcggagcaa gccgccggtc
     5041 taccggttca gctcggtcga attcatcctg cggatcgtcc gcaccacgct gcgcgtcaag
     5101 cggccgcagg actggctgtt gctgctgttg cggaccgctc tggtcgtcac gctggtgctg
     5161 ctcttcctcc ggccgctcta cttcgcgagc cgcgccctgg ccgggccgct ggggcggaag
     5221 aacgtcgtgg tgatcgtgga cgcgacggga tccatgggct gcaacgaggg cggacgcacg
     5281 cggttcgcca ccgcctgcgc cgaggcctcc gaggtgctgg ccgggttgac gtccgcggac
     5341 cgggccaacg tggtctggct ggacgccgag cccgacgccg ttttcccgga gatgggcgtc
     5401 aacatggggt acctccgcgc cgccttgagc cgcgccgaac tgcggccgca gaccggcgac
     5461 atcgcgggcg ccctgcggct cgccgtttcg atgctggaga accaggacgg ccgccgcgag
     5521 atctgcctgg tctccgattt ccagcgcgcg gcctgggaaa aggccagcct ggcggtcccc
     5581 gattcgatcc ggttggtcaa cctgtgcgtg gcgcagggcg acggggagaa cggcgcgctg
     5641 acggacctgg tcgtcgatcc gccggccccg ctgaaaggcg aggaagtcac cattcacgcc
     5701 gaggtggcca actactccgc ccggccgcgc cagcaaaccg tcttcctgga actcggggag
     5761 ggccgctaca accaggacct gatgatcccg ccgtgggaaa aggccggcgt gtcgttccgc
     5821 catcgtttcc gccagcccgg catccacacg gcgaccgtct cgctgaacga ggatcgcttc
     5881 accgccgatg accgccggtg ggccgtgatc caggtggcgg acagcctgtc cgtcggggtc
     5941 tccggcacga acacggcgga aggccggttc tggctccggg ccctcggggc gttgtcctgg
     6001 gtcagcgccg aaccgatcga tcccgcgcgc ctgcccaagg aggcggccta cgacgccgtg
     6061 ttcctggcgg atgggaatgg cgcggacgcg gaccggttcg cggatatgtt gggccggggg
     6121 tgcgcgatgg tctgcgtgcc gtctcgcgga acgcccgtgg gcccgctgct ggccgcagcc
     6181 ggcgtcgacg cttcccgggt tcccgccgag gccgcctggg agatcctgtc cggcccgacg
     6241 gccgcccgcg tggcgcgtaa ggaggaccgg ctcttccagc tcttcgcggg cggggaatac
     6301 ggcgacccgg ccggcggcgt gttccgtgcc cgctggcgtc tgcccggcga cgccgcggcg
     6361 gcgggcgaga cgctgatcgc ctacgaggat gggcttccgg cgctcgtccg ctaccggcac
     6421 ggcggcctcc tgtacctgtg ggccctgccg ctcgatccgg aggtcagcga ctgggcggcg
     6481 cgcgtcgagt tcgttccgtt cctgggcgaa ctgctgctgt ccagccggag cgcccgggga
     6541 ggcggcgcga cggcggccat gcccggcgac cggctggctt ggcgcgtgga ccgggaaatc
     6601 ctggcgtccg acgtaacgct tcagcacggg gccggggagg cgcttcccgt cctggcgcag
     6661 cgcgaggcgg aggggacgcg cgtggtttcc gagtcggcgg ccgacaccgg cgtctacacc
     6721 tggaaatacg agggtgaggc gcagcaggtc acggcggtca acttccccgc gctcgagtcg
     6781 gacctgcgtc cgatcccggt gaaggaaatc gagcagcagg gccgcgtggc gctggccgcg
     6841 ggccggcgcg cgcgggagtt gcgggacggg ctcccgttgt ggccgtacct gctgctggcg
     6901 gtggtcggat gggcgttgct ggaaggcggc gtgcaagtgt gggcggagcg gacatgaacc
     6961 ccctgctgcc gatagaggac ctggcgctct gggcggccgt gcttgccgcc ggcgcgggct
     7021 tcctggcgtg gcggtcgtcc gccaagtgcc ggccgcgcat ccgcctgctg ctggctgggt
     7081 tgcgggtcgc cgggatggcc tgcctggccg tcatcgccct gaatcccggc cgctggtccg
     7141 acctgtcgcg gcggaatccg tccgagttgg ccatcctcct cgaccggagc gccagcatgg
     7201 ccacccgcga tgccggggag gcgacgcgct gggacgcggc ctcggcgctg gtccgggacg
     7261 tccggcgccg cgccggaggt ccggaacata caccattgta tgtattcgcc gacgacgtcg
     7321 agccggccgg cgtccgcgat cccggggatt tcaaggccga cggcggcgcc acggacatcc
     7381 gccgcgccgt cgaaggggtg ctcaacctgt cccgctccgg cggccggaac ctggcgggca
     7441 tcctgctcct gtccgacggg cgccaagtcg ggggcgcggc ttccggggac gcggcgttgc
     7501 gcgcccgcgc gcgggatgcg ccggtgtttc cgctggcgct cggcagcgcg atcgagcgca
     7561 aggacctgtc gatcgagccg ctgcgcaggc accatgtgac gttcaagggc cagcgcacgc
     7621 gcctcgtcgc gcgcgtccgc gcctcgggcc tgggccccat ccgtcccgcg gtgcgcctgc
     7681 tggacgggga cggcgcggtc ctcggcgagg agacgctgtc gctcgagggc gacggcgagg
     7741 cgacggtgtc cttcgcggtc acgcccgagg gccggggcca ccaggaatac cgcttcgaga
     7801 ccgccgggtg ggagggggag agcacgcgcg cgaacaacga ggcgcgcgtc ggcgtggccg
     7861 tcctggacgg ccgcatccgc gtcctgatgg tcgaaggggt cccgtactgg gacagcaagt
     7921 tcatcgcgca gctgctgcgc cgccagccga acatcgaggt catctcggtg taccggctct
     7981 ccgccgatcg cttcttccgg gtggaaaccg acctggccga cgcggcccag atcacggggt
     8041 cggtcttccc cgacagcgcg gccgagctcg acctctacga cctcgtcgtg ttcggaaagg
     8101 gcgcggagta cttcctgact cccgagcgca tccggctgct ccgcgatttc gtgcgcgacc
     8161 gcggcggcgg cctgctgttc gcccggggca aggcctacaa cggcgagttc cccgagctgg
     8221 aggcgctcga gccgatggag tggggggagc gcgtcgccag ccccttcagc gtccggccca
     8281 ccgacgcggg ctcggcggcg ggcctgttcg gcgacatgct gcccggcccg gaggacgcgg
     8341 tctggggccg gctgccgccg ctccagttcg cccagcgtgt gcgcaagctc aaggccttca
     8401 gcgacgtgct ggtggagggc cacagcgagc tggccggcca tgagcagtcg ttcccggtcg
     8461 tcgtccggcg gcgcttcggc aagggcatgg tcgtggtcgt gaacgcggac gggctctggc
     8521 agtgggactt cttcccgtcg gtgaaggacg caagcaacat gtaccaggaa ttctggtcgc
     8581 agctggtcca atggtcggcc accttctccg aattccttcc gggctcgcgc tatgccctcg
     8641 gcttgagcgc ccgcgtggcg catcccggcg agccggtccg cgtgcgcgtg acccagcgcc
     8701 tgccggagcc cggcgcgccc gccccggagg tgcgcgtgca gcagggcgcg gagccggcgc
     8761 gcaccctctg gcccgcgccg gtcccggagg agcccggccg ctgggaagcg gtcttttccc
     8821 tcgaccggcc cggctcttac caggtcgcgc tgcgcgcccc cggggaacag gacgcggcgg
     8881 ccggcgtgtc cgacaccctc gaggtgctcg ccgcgccgcg cgaagaggac aatgtcagcg
     8941 ccgacccgga tttcctccgc gagctggcgg ccctatcggg cggggaggtc gtgacgcccg
     9001 aaacgctaga cgtcgtggtc cgccggttga aggagggcga agaggacgtg aagtccggcc
     9061 gggcgacctg ggagccggcg tgggaccggg cctggttcct ggtcttcacg gcgttctgtt
     9121 tcgcggcgga atggttcgtg cgaaggagga acgggctgct gtgaccgcac acgacaaacc
     9181 gaccgaactg ggccggctgc tgcgccagcg cctccgccaa tggcaggcgg gcctggcgca
     9241 gctggcgctg ctccaggcgc tgtcgcgcgc catccttgtc ctgctcggtt acttcgtcct
     9301 cgattacttc ctggccctgg gctccgcggc gcgcttcgcg ctgggcgccc tgctgctcgg
     9361 cctcctggcg gccgtcctgg ccgcccgcgg catccgcatc gcggcgctca cgccgcgcga
     9421 catggccgtc cgggcggacc gccggatcgg gggccgccgc cgcgccgtcc tgggcgcgct
     9481 cgaactcgag acctggctcg ccgggggggc cgcgcgggag gccgggttga tggcctatct
     9541 cgctgggcag gcggtcggcc gcgccgcgga ccaggtccgc cgcctcgatc tccgtgacgc
     9601 gctcctggag gaggaattct gccggcaccg gcgcgtcctc tacgcctgcg ccgcggcggc
     9661 cgcgctcgtg ttcctcgtga acgtgcccgc gacgcgcacg atcctggccc ggatcttcct
     9721 gcccggccgc gatgtccctc cgtacagccg ctacaccttc caggtcgagc cggcgagccc
     9781 cgtggtgcta tacgggggcg acctggcggt tcgggtggaa atcggcgggc ggaccgtcgg
     9841 cggggaggcg ggcttcctga cccgcgccgg cggacggatc caccgcgcgg cctgcttcca
     9901 ggaaagcgcc acgcgctatg cccagcggct ggaaggcgtg atcgagccgg tcgagttctg
     9961 cttcaccgtc ggccgcgccc ggagccgctg gtgccccgtg gacgtgctgt tcgacccgca
    10021 catcgccgcc gcggaggtcg tgctcgtgcc gccggcctac agcgggaagc ccgcccgccg
    10081 cttctatctc ggcgcggagg acgtgagcgg cctgaagggc acccgggtcg agctgacgct
    10141 cacgagcaac cggccgctgc gggacggggt cattcattgg cgtttcgaaa acgacaacga
    10201 ctcggcgccg gcgctttccg gcgagaaggt gggcgaccac accgtgcgtt tcacgtggat
    10261 cctgcgcgcc acggcgacgg cggagatcac cgtgcgggac atccgcggca cgcccaacgc
    10321 atcgccgttc aagatggccc agcgcgtcgt gccggacgag ccgccgtccg tcgccttgag
    10381 cgagcccccg ccgttctccc tggccacgcc cggcatcacc gtcccggtcc gtggcgcggc
    10441 cgacgacgat ctcggcctgc ggcaggtcga cctggcccga gcgctggtcg gctaccgcga
    10501 tcgcctgcag acggtgggac cgcccgagcc cgcctccggc ttcgagtacg agggcgaact
    10561 ggcgctcccg aagctgggcg tcgaggcggg ccaggtgctg gaactgtacc tggaggctat
    10621 ggacagcaac ccgagcctcg ccggcatatc ggtctccgag gtggcccgca tccagatcat
    10681 ctcggaggaa gaatacgccc tcatgcttcg cgtccggatc tcggccgagg aattcgcggg
    10741 ccgtttcaac gaggtgcacc gcgatttcac gaacctgcag gaggccctgc gtgcgttgag
    10801 cgacgagctg ggcaagagcg agcgggacgc cgacgcggtg gagcgggcgc gggaggaggc
    10861 tcgccgcgcg gccgggcgcg ccgcgaaggc gttcgacgag gtggcccgcg atttcccggc
    10921 gttcgacatg gagcgccggc tccaggaagc ggccgccggc atggcggaca ggatgcggga
    10981 gaccgaggcc ctgctggccg gcagcccgcc gccccacgac gggctccagg agcaggtcga
    11041 cgcgttcctg gccgaatacg ccgcgggccg ccgcgcgctg gacgagcagg tccgggatgc
    11101 cggcgaggcg gccctcctcg cggagatcat ggaaagcgcc gcccactatg cacggctcct
    11161 cgccgagcag gaggcgctcg tgcgccggct ctcgcgtttc gagggcggcg gacggaaaga
    11221 ggacctcccc atcctggccg acttggaaag ccggcaggcc gatattcgcc ggcaactgac
    11281 cgaattgctc gaccggatcg acgcgcaggc gggccggctc cggctggact atcagcaact
    11341 gacggccgac atggaggcct tcgtcgagaa ggcccgcgag ttggagatcg tgccgacgat
    11401 ggaggacgcc gagctgaagg cgcgcaacca cgacggggcg gagacctgcc gcctggccgg
    11461 ccgggcgctg gagaaaatga aggagctggc cgggcccgaa aagggcaact gtttcggcgg
    11521 catgtgccag ggccagctgc gcttcgacgt gcggcagaac atgcgctcga ccctcgagca
    11581 gatgctggcg gccatcacga tgcggatttg cagcggggag ggctccatgc cggggatggg
    11641 acgcggcggg tccggcctgg gcggcggcgg ggacgacggg tattggatgg gcgggcagtc
    11701 gccgctggac gtggcggtct acgggcccga acggcgcgcg taccgatcct cgcccgccgg
    11761 cgggtctggc gccggccggg ggaagggccc gggcgaccac cggatcggga aacaggaaag
    11821 cgaccgcgtg gcgcccgccg cctccggccc cgtcgcggcg gaaagcatgc cgctggagaa
    11881 cgtgcccgag aattaccggg aatcggtgaa gcgctacttc agcgcggagg agccatgagc
    11941 atgaaacgga ttctcttcgt cgccggcctc gcggcgggcc tcgccgcggc cgcccctgcc
    12001 aaggagggcg tggtccaatg cgccaacctc atctacggcg gcacccacac gtcgcgttgc
    12061 ttcagcgacg agttcttgag cgccgtccag cgcgacacgg ccatcgccac ggagcggcgg
    12121 ttcaagagcg tcaagctcgc ttccgaggac ctgttcagct atcccttcgt catcatcacc
    12181 ggcgaggccg agtttcattt caccgcgaag gagcgcgagc atctcaagaa gtatctcgag
    12241 agcggcggct tcctactggc gtcggccggc tgctcgaaca aggactggga tcgcgccttc
    12301 cgccgggaga tgaagaccct cttcgcggaa aaggggctcc gcccgatcga gatggatcat
    12361 cccctcttcc gcaccgtcca cgccatcacg gagctcaagc tccatcatcc cgcggcggcg
    12421 gcccgcctgg aaggcatcga ggtggacggc aagctggtgg tggtgtactc gccgcacggg
    12481 ctgaacgaca cggcccacac cgaggggtgc tgctgctgcg gcggcaacga gatcacgaac
    12541 tccctgcagg tgaacgtcaa catcctggta tatgcgctgc tttactgatc catggcggtt
    12601 cgggaaggtc ctgggcctgg cgggcctgct ggcggccgcg cgttcgccgg ccgcggagcc
    12661 gccgccggac gcggccccgg ccaccggcgt cgtgacgctg gtctggttcc cggacctgaa
    12721 cgccgcgctc aaccgggcgg aggcggaata ccggcccgtc gtgacgctct tcacgtcgcc
    12781 gggctgcggg tggtgccgcc ggctcaaggc ggaaacgctc gatgatccgg aggtccgcaa
    12841 gctgctccag cattttgccc tggtggagat cgacgtgatg gaggaccccg attcggcgca
    12901 acgctacagc ctgcgcggcg tgccgaccct gctgtttctg gcctcggacg ggcgggtgcg
    12961 cggcggcgtg tccggctacg tctccgcgcc ggaactgtgc accctgctgc aaaaagagct
    13021 caacccggag ttcctggccg gccaggatgc gccctatcgg gacctgctca aggccctcga
    13081 tgcccggaag gtcccggacg aaatgtggcc tgcgatcctg gccggaatgg gcgaggcgca
    13141 gaaacggacc gaactgcgcg accggatatt cgccctgaag ccgtttcctt ccgcgtctct
    13201 cgtcgagcgc ttgaaggatg cgcgcctggc ggtccggctg ggggccctgg agatcctcga
    13261 ggagatggcg ggcgcctcgc gggatttcga tccctggcag tctcccgaag gcgaggccaa
    13321 ccgggaaccg ctgcggcagt ggcaggagtg ggcctccggc gcgaccggcg ccgtggcccg
    13381 ggtttatgcg gtcctgacgc ccgaggaaat ccggaccagc ctgctggacc tcgtatccga
    13441 ccaccgtgag cgttcgctcc gcgccatgcg cctcctggag catggcgggc cggggaccgc
    13501 cgatgcggtg gcggccttcc tgtcggggca ccccgatctg ccggcgggcg cccggggacg
    13561 catccgggag cttcagtacg tcctgctgct gccggaactg gcggggacgg acgtgagggc
    13621 cttggcgcac cggctggttt tcggcaacct ggatacgcgc ttgaaggcca tccaggacct
    13681 gccgaagttc cgggccgcgg ccacgatcgt cctggcggac gccctcgagg atcccgagcc
    13741 gctgatccgg gaagcggcgg tggacagcct ggcgcgggcc gacccgcggc gcgcgatgga
    13801 catcctcggc cggcacgtaa gcgtcgaaaa ggacaacgag gtggttttcg cgatcctccg
    13861 cgtcctgggc ggcctgaccg gccggaaggg cctggatatc ctgtcgtcct atctccagag
    13921 cgaaaacgag gatctggtgg tcgccgctct gcaaagcctg ggacggctca agacccaggg
    13981 cgtgtcggac aaggtcggcg cctgcctgaa agatacccgc tggcgggtgc gggcggccgc
    14041 gctcgaggcg gccgcgagct tgaaagccaa cagcctcgcc gaccaggtca tggcccttct
    14101 cgacgatccc gatccgtttg tccgcgattc ggccatccat accctggccg cgctgggcgc
    14161 caagagggcc gcgggccggc tggagaagct cttcctggaa aacgatgaac tcaagggccc
    14221 gatcgtcagc gcctttctcc agatggagct gcgcctgccg gtatccttcc aggcggcgct
    14281 gaaagaccgt ccgccgaacg tgctgctctc ggtcatcgag gcggtcgtgg aagacggcga
    14341 gttcaaggac ctctcgctga tcgctcccct gaccgatcat cccgatctgg acgtggcgtg
    14401 cgcggccatc cagcccctgg cgcagaaggg catgagccac gcaccctacc ggcagcggct
    14461 ggcggacatc ctgcgggccg gacagcctga aaaagtggtc accgtgctcg aatccatcga
    14521 gatcgaggag tcgaacctgt cgccgtccta cgcgacggcc gtggactttg acgccctcgg
    14581 cgccgaggga ggctccggag cgggaaccaa ctcgttgacc gaattattcg aggcgttcaa
    14641 gccgggcggc gcggagccgc ccgcgccggc gcccgccccg gcggaaacgg gcgccgccgg
    14701 cggcctgctc aaggccctgg gattcaagac cgccgcgccc gcccggtccg cggtgtcggg
    14761 tccggactcg ctcgccgacg ccgtgcagcc ctatgccgga ccggaatacg agggccggac
    14821 ccgcttcgcg gcggcgctcc tgttgacgag cttcggccag accggccagg tggctttcct
    14881 gcaacagacg atgggcgacc gaaccgagga tgagcggcag caggtggcgc agtgcgtccg
    14941 tccgcgaatg cgggacgcct tcctgccgct cctggacgcg ctgctggacg acgagtcgtc
    15001 tgccgtgcgg gtcgccgccg tgtccgccgc cgttgagcgc ggcgggagcc gcggcctgga
    15061 gcgaatgatg gcggcgctgc tccgcccggg cggccggctc aagccggggg aggtgtacag
    15121 ccacgacatc gagtccgtcc tgcgccggca ggaggcgcgg ccgaggatcc gcgagtgcgc
    15181 catgctgctt ctgcaacaga gcaatgcgcc gcccaggcag gcgctcgggc tggtgctgct
    15241 ccagaaaacc tggcaaccct ccgaccggac cgcggtgtgg cccttcctgt acgcgggcga
    15301 tccctgggtg cggcgggcgg cctttcacgc cctgggccag aacgacaagg catcgttccg
    15361 gaaggtgctc gactgcgccg tggcggattc ctccgagtat gtccgggccg tggtgcccaa
    15421 ggtttatgag cggggcatgt cccgctggac gcattacttc agcctggatg aattcgaagc
    15481 cgatcaatac tactggtccg tgcaggacaa ctcggcgcgc ctccaggaca tggaggtggc
    15541 ggccctgcgg actctggcga aggatgccga cccgcgcatc cggatagaaa gcctgatggc
    15601 cctgctttcc aaccgccagg gggtcgacct tgcggaactg gtccgggccg cggaggccgt
    15661 cccggatcgc cgggcggtgg cggaccgcct gggccgctac ctcacggata actccgcgag
    15721 gctcgggccg gaattctcgg tgctgttgcc cttcctggac gaatccgagc agaatgaaag
    15781 ccagaaggag aaggtccgca agatactgga gaagaaccgg ccggccgggg aactgccggt
    15841 cgtcgccgct ttggatcgtc ccatcgaggc ggtcattcgc gatgcgcaga cagcccccgt
    15901 gccggaggtg gccgcgccga gtgtgcgcgt gatcttcttc cgcagctacg ggtgccgcga
    15961 ctgcgagcgg gcggaggcga tgttcccgca attgagggag gtgttcccgg gcctccagat
    16021 cgacatccac gacatccgga agacccgggc catgagcctc aacgaggccc tgtgcgagcg
    16081 gttcggcgtg ccggaccgga tccgcctggt cacgccggcc ctgttcacgg cggccggcta
    16141 cagcatcaag gaggacatca cgttcgcgcg gttgggcgaa ctgctgacgc gatccgccgg
    16201 ggcggacgac gcctggagcc gggtggagga agccgaactc gaccaggcgg ccgcttccat
    16261 cggcagccgc taccgggata tccgtctcgg ggtgatcgcc gcggccggcc tgctggacgg
    16321 cgtcaacccc tgcgccttcg cgacgatcat cttcctgctg tcctacctgc aggtcacccg
    16381 gcgcacctcc cgccagatcg cccaggtggg cctggctttc atcgccgggg tgttcgtggc
    16441 ctactatgcc atgggactgg gcctcgtgga gatcgtctcc cggctcggcg tgctccgccg
    16501 cttcagcctc gtcgtcaatc tcctgctcgc ggcgttcacc ctgctgatcg cgatcttgag
    16561 cgtgcgcgac ggcatccgct gcctccgcgg ccggctggcc gacatcacgc tgcagctgcc
    16621 cggcttcctc aaggaccgga tccacacggt catccgggag ggcgcgcgcc acgcccgttt
    16681 cgtggcggcc gcgttcgtgg ccgggctggt cgtctccgtg ctggaactgg cctgcaccgg
    16741 ccaggtctac gcgccgacga tcctgttcat gctcaagacc ggcgaggagc gtttcgcggc
    16801 catgggatac ctgctcgcgt acaacgccgc cttcgtcgcg cccctgttcg tcgtgttcgc
    16861 gctggcggtg ttcggcctgc gcagcgacgc gctcatccgc ttcatgaatc gccacgcggc
    16921 gggcgtgaag ttcgcgaccg ccgggctgtt ccttctcctg ttcgcggtct tcgtctggag
    16981 cctgcgcgga tagccggcgg ccggctacag tccgcagcgg tccgaatccg attcgccctc
    17041 gacgtcctcg gcctggacgc tgacgtacac gttggtggtg tagcccggcc ccgtgaggtt
    17101 ggtgaaggtg agggtgatcg agtactcggt gctggtctcg gagaagaccg ccgggctgat
    17161 cacgaccccg ttgaagttgg actggtcgag cgtggccgtc gcggtcgtgc cggtcggggt
    17221 atgcagcttg ttccagtaca tcatgtcgaa ggtgtgcgag tcgtacacca cgtaggccgc
    17281 cgggttcgtg gaccagcggt agaccgtcgc cttgaccgtg ttcaggtcca gttcctccgc
    17341 cggggtggtg acggtgacct gcacgaccgg gcgggagggc gtggaggaat ccacgctgtc
    17401 gatctggaca tagggcggcc cggagttgga gtagggcatg gccaggaagg gattcacgca
    17461 gttggactgc caggaactgc tgtcccggat ctcgaaatgc aggtgatcga acccggacgt
    17521 gctgcacccg gagtcggcga tccactggcc ctgggtgacg gaatcgccca cgttcacgta
    17581 ccagttggtg atgtgctggt agttgctgta gtagtagccg ctgccgtcgg cctggcggtg
    17641 cttgagttgg acgagcgcgt cgctgtagct gttggtgaac cccgccttgg tcaccgtccc
    17701 ggacgcgatc gcatagaccg gggtgaacag cggcgcgggg atgtccacgc cccggtgcca
    17761 gtcgtagcgg tagctttccg aggctttgag ccgggggccg tagggcgagg agaacggggt
    17821 gtccggcgtc gtcgagcggc cgatgggcca gaggtggtcc tggtacggcc cgagctggtc
    17881 caggtaggcc gtgcgcaacc gctcctgcag ctggtccgag aagttcagga cgaggcgaac
    17941 gatgcccgag ttggtgatcg taatggccgc ggtctcccgc gccggccccg cgagaacggt
    18001 cagaccggcg atgatgcaga cgatgcccgc ccgtgagttc atggagtgcc tcccaccgct
    18061 ggttcgcttc tgcccttgaa catgccccgt tcgcggcgtc ctgttaagcc cggattcgca
    18121 aggccatccg gggcccggat ggcggccggc ctggtcaggc gcgtccggac caaaccggtt
    18181 aaggggccgg cgggttaaaa aacgttgcga aagccggatt ctttgtgtat gaatccctgt
    18241 cccttggaat cagcaaaggc cgctttctgg aatccgaatc ggtaaaggaa cgcgctatga
    18301 aggaattggc gatcggtaac gtgcgcaacg tggtgctcgt gggtcacacg ggcgccggca
    18361 agaccagcct gctcgacgcg gtgctgtaca agctcggtgt aaacgaccgg ctcggttcgc
    18421 cggccgaggg caccagcgtg gcggactgga gcgacgagga gaaggatcgc aagatcacga
    18481 tctgggccaa gcccttcaac gccgtctatg cgtccgcgca gggcgggaaa tacgacctgg
    18541 tcatcaccga cacgcccgga tacgccgatt tctacggcca gaccgtggcc gcgaccgcgg
    18601 tggccgacgc gggcctcgtg gtcgtggacg cggcctcggg catccaggtc ggcacgaacc
    18661 gttcgtggcg gcgctgcgag acgctcggcc tgccccgcgg catcgtcatc accggcctcg
    18721 acaaggagaa caccagtttc gacgacaccc tggcgggcct gcgcgccgtc tggggcgacc
    18781 gctgccgccc ggcgaccgtc ctcggcaagg acaagaagtc ggtcgcggac gtgttcaccg
    18841 gcggcggcga ggtggaatcg ctcaagagtg ccctggtcga ggcggccgcc gagaccgacg
    18901 accagctcat cgagaagtac cttggcggcg agaccctggc gccggcggag atcgcggacg
    18961 gcctgcgcaa ggccgtggcc acgggcaagc taatccccgt tttcgtaaca ctggcgaaga
    19021 ccggcgcggg tatcccggaa ttgctcgagg gcttggtgcg cttcttcccg tccccggccg
    19081 accgcgagtt caaggacgcc gagggcgccg tcgtggccgc gggggccgac gccccgtttg
    19141 tcggccaggt ctggaaggcc gtgaacgacc cgttcgtcgg ccagttgacc atcctgcgga
    19201 tcttcggcgg cacgctccgg tccgacagcg aaatctacaa cgcgacgaag gagcagaagg
    19261 agcgcatcgg cccgatctac ctgctgaacg gcaagaagca ggaatcggtg gccgaggccg
    19321 gggcgggcga gatcgtcgcg ctggccaagc tgaaggccac cgtcatcaac gacgtcctct
    19381 gcgcgcccgg caagagcgcg aaactgacgc ccatccagtt cccgaacccg acggccgcgt
    19441 acgccgtggc gccgaagacg cagggcgacg aggacaagtt gagcacgggc ctgcaccgcg
    19501 tggcggacga ggacccgacg atccgcgtcg aacgcaacac ggagaccaag gaactgatcc
    19561 tgtacggcat gggcgacctg cacctggaca cgaccctgca gcgcatgaag aagcgccaca
    19621 acgtcgagat cgcgttgagc accccgaagg tcccgtaccg cgagacggtc acggcgctgg
    19681 gcgaggggca ctacaagcac aagaagcaga ccggcggccg cggccagtac ggcgaggtct
    19741 acctgcgcgt gcagccgatg aaatccggtg aaacggaatg gttcgaggac gccctcgtcg
    19801 gcaccaacgt cccgcgcaac ttcctgcccg cgatccagaa gggctgcgtc gagggcatga
    19861 accgcggcgc cctggccggc tacccggtga cgaacaccaa ggtcagcatc tacgacggct
    19921 cgtcgcacga cgtggactcc tcggaaatcg ccttcaagat cgccgctgcg cgcgcgttca
    19981 gcgacggcat gagcaaggcg cgggccgtcc tgctcgagcc catcgtcacg gcgaagatca
    20041 tggtgcccga ccagtacatg ggcgacatca cgggcgacat gaaccacaag cgcgggcgca
    20101 tcctgggcat cggcacggag gacggcatgc aggtcatcac cgccgaggtg ccgcaggcgg
    20161 agatgttccg ctacagctcc gagctgcgga gcatcaccgg cgggcgcggc tcgttcgaga
    20221 tggaattcac ccggtacgac atcgtgccgt ccaacgtcgc gcagaagatc atcgccgagg
    20281 cagcgaagaa caagaaagca gcggaggaag agtaacccgt agccggtcac cggtcacttt
    20341 aactggtcac ttattgccgg ttactggtca cggataaccg gtcggagagg agcggaccat
    20401 gagcggtcac agcaagtggg cgaacatcaa gcacaagaag ggcgccgccg acgccaagcg
    20461 cggcaagatc ttcagcaaga tcgccaagga aatcatgatc gtcgtcgggg cctccggcaa
    20521 ggacgtcgcc tccaacccgg ccctgcggac gctcatccag aaggcgcgcg ccgtcaacat
    20581 gccggcggat aacatcgacc gggcgatcaa gcgcggcacg ggcgaactcc agggcgcgaa
    20641 actggaagaa atcatgttcg aggggtttgc cgccggcggc gtggccctga tcgtccaggc
    20701 cctgaccgac aaccgcaacc gcacggtcgc ggaagtccgc cactcgttca ccctgcacgg
    20761 cgccaacctg gcggggcagg ggcaggtgat gcgcaccttc cagcgcaagg gccagatcct
    20821 ggtggacgcg gcgggcgtgg acgaggacaa gctcatggag ctggtcctcg aggccggcgc
    20881 cgaggacatg cggcgcgagg aggaccagtt cgagatcatc accgatcccg cgcagtaccc
    20941 ggccgtggcc gaggcgatca acaaggccgg gatcaccgca gcgagttccg aggtcacgct
    21001 gctgccgctg acctggaccc cggtgacgga caaggccaag gcgtcgtccc tgctcgcgtt
    21061 catcgacgag ttggaggagt tggacgacgt gcagaacgtc tactccaatt tcgatatccc
    21121 ggccgaattg ctcgaagccc tcaacaagta gggccggcct ccggctccca tgagcgtgct
    21181 gggcatagac gcctcgctcc ggtccaccgg ttacggcgtc gtcgcccggc agggcgcctc
    21241 cttccgatgc ctggaccagg gcctcgtccg caaccccccg gcccatctcc attccgaatg
    21301 cctcgcggcc attcatgccc gggttaccgc cctgctggag acccatcggc ccgaggcggt
    21361 ggcgctggag ggcggcttct acttcaagaa cgcgaagacg gctctgatcc tgggcgaggt
    21421 gcgcggggcg gtcatcgcag cctgcgccct gcgcggcgtg ccggtctatg aatacgcgcc
    21481 ccgccgggtg aagcagggat tgacgggcgc gggcgcggcg tcgaaggagc aggtcggccg
    21541 catggtcgcg tccatcctgg gcctgaccga gttgccgccc gaggacgccg ccgacgcgct
    21601 cgcgatcgcc ctctgccact tccacggccg caccggcatc gcggccctgc agccgaagcc
    21661 gatctgaagg ccgccgggtc cgtctccgct ccctgcaaca ttgaaatgtc gccgcgttcg
    21721 cgtaatcata aggttaaatc agcggagtga ggcttatgca actggatcgt ctgaccatca
    21781 agtcgcagga ggcgctgcag cgggcgcagc aactggcggg ggattacggc cacgccgagg
    21841 tggacgtgga tcatcttgtc ctggcgctga tcgaccagga agacggcgtc gtccgccccc
    21901 tgctcgagcg gctcgggatc cccgccgagc aactgcgaga gccgctggag cggtcccttt
    21961 ccgcccgcgc ccgcgtcgag ggcggcgcgg aacgctacct gtcccgctct cttcgccagg
    22021 ccctggacgc ggcgttccgc gtggccgaga agatgaagga cgagtacgtc agcggcgagc
    22081 acctgctgct cgccgcgctg ccgcaagcgg acagcgagac cgcgcgcgtc gccgcccggc
    22141 tcggcctgac gccggacgcc gtcctcaagg cgctgcaggc ggtccgcggc aaccagcggg
    22201 tgaccgaccc ggccccggag gacaagtacg aggccctcaa gaaatacggg cgcgacctga
    22261 ccgaggccgc gcgcaagggc aagctggacc cggtgatcgg ccgcgacgcc gagatccggc
    22321 gggtggtcca ggtgctctcg cgccgcacga agaacaaccc cgtgctcatc ggcgagccgg
    22381 gcgtcggcaa gacggcgatc gtcgagggcc tggcccagcg catcgtcgcg ggcgacgtgc
    22441 cggagggcct gaagaacaag caggtggtct cgctggactt ggggtccatg atcgcgggcg
    22501 cgaagtaccg gggcgagttc gaggaccggt tcaaggcctt cctcaaggag gtcatcggcg
    22561 cggagggccg gatcatcctg ttcatcgacg agctgcacac gctggtcggc gccggccggg
    22621 ccgagggcgc ggtggacgcg tcgaacatga tcaagccgcc gctggcgcgc ggcgagctgc
    22681 gctgcgtcgg cgcgacgacc ctggacgaat accggaagca catcgagaag gacgccgccc
    22741 tcgagcggcg cttccagccg gtccaggtca acgagcccac ggtggaggac accatcgcca
    22801 tcctgcgcgg gctgcgggag cgctaccagg tgcaccacgg cgtgcggatc caggacggcg
    22861 cgctggtcgc ggcggcgcgg ctgtcgcacc gctacatcag cgaccggttc ctgccggaca
    22921 aggcgatcga cctcgtggac gaggccgcca gccgcctgcg catggagatc gacagcctgc
    22981 cgaccgagat cgacgaggtc gagcggcgga tcatgcagct cgagatcgag cgggaggcgt
    23041 tgcgccagga aaaggacgag gcgtcgaggg aacggctggc tgcgctggag aaggagctgg
    23101 ccgagcagcg cgagaagagc cggggcatga agctgcactg gcagcaggag aaggagcata
    23161 tcgccgagat ccgcaagctc accgaggccc tcgacctcct gcgcggcgag gaggaagccg
    23221 ccaagcggaa cggccagctc gagcgcgccg ccgagatcct gtacgggcgc atcccgaaga
    23281 tcgaggcgca ggtcaaggcc gcccagcaga agctcgccga cctccagaag aaccagcgga
    23341 tgctgaagga ggaggtggac gccgaggaca ttgccgcggt cgtctcgaac tggacgcaca
    23401 tcccggtgag ccggctgatg gagacggaga aggccaagct cctgcacatg gaggagcggc
    23461 tccggaagcg ggtcgtgggg caggacgagg cggtggccgc ggtctccaac gccgtgcggc
    23521 ggtcccgctc cgggctccag gacccgaacc ggccgatcgg ctcgttcatc ttcctcgggc
    23581 cgacgggcgt cgggaagacg gagctcgcgc gggcgctggc cgagttcctg ttcgacgacg
    23641 agcacgcgat ggtccggctg gacatgtccg agttcatgga gaagcacacc gtcagccgga
    23701 tgatcggcgc gccgccgggc tacgtcgggt acgaggaggg cggctacctg accgagcacg
    23761 tccggcgcaa gccgtactcg gtcgtccttt tcgacgagat cgagaaggcg cacccggacg
    23821 tgttcaacgt cctgctgcag atcctggacg acggccggct gacggacggg caggggcgca
    23881 cggtggattt caccaacacg gtggtcatca tgacctccaa catcggcggc ccggcgatcc
    23941 acgaggcgct gcagcgcaag ccggacctga aaagcagcga ctcggcctac gagcagctgg
    24001 agagccgggt gtacgaggag ttgcgggcgc agttcaagcc ggagttcctc aaccgcgtgg
    24061 acgagatcgt gatcttccac agcctgggcc gcgagcagat caagcagatc gtcgagatcc
    24121 agctggcgcg cgtccggaag aacctcgagg agcggcacat cgcgatggac ctgaccgatg
    24181 ccgcccggga tttcctggcc gaggagggtt acgacccgtc cttcggcgcg cggccgctca
    24241 agcgggcgat ccagcggcgg atcctggacg cgctggcgac ggagatcctc aagggcgcgg
    24301 tggccgaggg cgaccgcgtg accgcggacg cggcccggga cgggagcggc ctggccttcc
    24361 gggtcgcgcc ccgccccgga acctgaccgc cgattgcgct ttattcgccg cggggggggc
    24421 gatatagaag aagcgcatgg acaccgtcct gtcagcggcc atgatcatcg gcatcttcgt
    24481 catggtgatc ctgatcacgc aggacatgtg aggaccgatg accgtccgcg aaatacaggt
    24541 cgaggtcaag ggcctggtgc ccacgcagag cggggtgggc gtgttcctcg gccatggcga
    24601 caaggtgatc gccatcttcg tggatcacag cgtggccgcc gcgatcacga tgttcatgca
    24661 caagctcaag aagccccgcc cgctgaccca cgacctcatc ggcagcatcc tcgcgggcct
    24721 cggcgtgaag gcccagaagg tcgtggtgaa cgatctgaag gacgacacct ttttcgcccg
    24781 gctgttcctg ctccaggagg gcgagttggg ccggaacctc gtggagatcg attcgcggcc
    24841 cagcgacgcc atcgccgtgg cgctgcagca gggctgcccg atctacgtga acacgtccgt
    24901 ctgggagcgg gccgaggaca tgtcctgggc gctcaagcag aacccgccgg aagcgccgtc
    24961 cacggaagag cactcctgat ccggcccgcc cgcgggcctc accgcatgcg gtggatgagg
    25021 atcagcccga cggccaggaa ggccaggttc ggcagccagc cgccgatcca gggcgcgatc
    25081 aactgcttct tgccgaaggc caggcccacg ttgacgaaga cgtagtagcc gaagaacagg
    25141 ctcaaggcca gcacaatgcc gaggaaggcg ccgtgccgcc cgctcttggc gccgaacggg
    25201 atccccagca gcgtgacgac gagacagttc cacggcatgg agaggcggtg gtgcaggttc
    25261 gcgcgcaccc gcgacacggt ttcgttcgac aggcgcttgt gggtcttcag gaaagtcagg
    25321 atctcgaggg acgagagata ctcggggtcc ttgatctcgt tgatgaagtc ggaggggacc
    25381 tcgttgtaat ccgtcatctc ccggtgaatc tcgaagcggg gcggccccag ggggttgctc
    25441 cccgcgtcgt acgcctggaa ggacaggtcc tgcagccacc accggccgtc gtaccagtgg
    25501 cccgacttgg ccacgatccg gtactcctcc gatccgtcgg gccgctgctg gatcacgttc
    25561 acaccgcgca tgtcgaaggt ctgcgtgttg aatgcgccga tcatccagtc gcgccggccg
    25621 agctcgttgc gatagggaag cggggagacc acgtgcgtgg agaggtcgcc cttgctcttc
    25681 tgcatcacga tgaactggtg cgcccagtac gcggaccagg ggccgagggt ttcgtggatc
    25741 accccgacga ggatggaggc ggacagcccg aggatcacga ggggcgccat gagccggtag
    25801 aggctgatcc cgcacgcgcg catggccgtg agctcgttgt tcttcgtcag gtgcgaaagg
    25861 ctgtagagca ccgcgagcat cagcgaaacc ggcgcaatga tgatgagcac ggacggcatc
    25921 aggaagcaat agaatcgaat caccgagagc aggggcgtgc ggccctcgat gaagtccggg
    25981 agattgtcga acaggtcgta gatgatgtag atcaggatga agcccgccag gcagtacagc
    26041 agcggcacga ggaccgcctt gaacaggtat ttatccacga ttttcatgct gtttccgggc
    26101 cggcgccgtt caccggccgc gaaactctag ccggttcccg gcggaacggc aagcgcggcg
    26161 ctactcggcc ttcaggtcgt agatcccccg gtccacgcgc gtgcagcgcg gcgtggcgat
    26221 cagcagctcc aggacgtacc cggcgccctt ccgcttggcc ggtttcaggg attcgttgaa
    26281 ggtggaggtg atcttccggt agtgcgccgg tcgatccagg cggcccagca cctccacgac
    26341 gtacgggccg gccccgttcc ggtagaggaa aaagcggcga tccgtcgtcg aggccaccgc
    26401 gggggcgtgg tcgagcaggc attcgatggc ggcggcgggc tcccggaggg aggccagggt
    26461 ctcggtcccc gcccgcttca tcagctcgtc gagcgagagg ggctggccgg cctgggacag
    26521 gatcgcggtc agccgggatt ccagcgcctg caggaaatcc agggagaagg tggagaa
//
LOCUS       DAOOTK010000034        25675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1340_length_25675_cov_5.763739, whole genome shotgun sequence.
ACCESSION   DAOOTK010000034 DAOOTK010000000
VERSION     DAOOTK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_25675_cov_5.763739"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /gene="aroA"
                     /locus_tag="PK634_05860"
     CDS             <1..1088
                     /gene="aroA"
                     /locus_tag="PK634_05860"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOW97296.1"
                     /translation="TGGKFLEPVDPLDLGNSGTGMRLLAGLLAGLPITAELTGDESLR
                     SRPMNRIKEPLEKMGAVVELLGEKGRPPIRIRGGNLKGMDYAIPVASAQVKSCILLAT
                     LFAEGTTTVTEVLPTRDHTERLFLNAGIQLEVDGLRVRMKGYGAKGPKLKARPWEVPG
                     DFSSAAYALAAVAARPDSSVTVSGVGLNPRRTALLDVLRRMNARVEVATRSGTDEAEP
                     FGDVTVSGARLTGTTVGGGEIPALIDELPLVAILGALAEGETVIRDARELRVKESDRI
                     TCMANNLKLLGVDVEEREDGMLIRGPARLQPVAGIQSYGDHRIAMSLAVLGLYGEGPV
                     CIHNIACVLTSYPAFWDHLIALGAHVE"
     gene            1078..1764
                     /gene="cmk"
                     /locus_tag="PK634_05865"
     CDS             1078..1764
                     /gene="cmk"
                     /locus_tag="PK634_05865"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOW97297.1"
                     /translation="MSNNHSVSVVAIDGPSASGKSTVSKRVAQELKFTYVDSGSLYRG
                     MTWKALRESVNIGQPEQVLDLMNRMKLEFYAENRVVLFTIDGEDPGLQLRSEPVRENV
                     ADIAAIPGVRAFLVARLQEMVRFGDIVMEGRDIGTVVFPESPFKYYLDADPEERARRR
                     SLELKNMEGVGDVSKVLDSLTRRDKKDSTRKTAPLQIALGAKVINSTSMTIEEVVAQI
                     VAEVRAALGT"
     gene            1761..2405
                     /locus_tag="PK634_05870"
     CDS             1761..2405
                     /locus_tag="PK634_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOW97298.1"
                     /translation="MKDRCLLYRFVRGSTAIWLKVWLKLESHGAENVPAAGGCVLASN
                     HASYLDPPIVACGLKHRIVRFMARDTLFAGTFRNWFFHGIRCVPLDRTRGDVAALRKG
                     IQILKEGEVLGLFPEGTRSPDGQLKPAKGGIGFLVAKAGVPVVPVYVGGSFEAFPKGA
                     QRIRRGKVFIYYGQPIAFEEIARLGQDRDSYQAIGDLIMSKIAHLREQALVSPR"
     gene            2674..3360
                     /locus_tag="PK634_05875"
     CDS             2674..3360
                     /locus_tag="PK634_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOW97299.1"
                     /translation="MIYTCWGVVLACGKDEQFTPEISTPFINLSGKPILVHALTAFEQ
                     CVDISYVLVVAEKTRIDSVLGMAQMYGLSKVKKVIAGFGSRQASVQAALKNLDEEANL
                     IVVHEGSRPFVKPELISEVIKSTKRYGSGVAAVPLADAVKETRKGLTVATSVTGGAYW
                     LAQTPAAFRRDLLEKGLKAAAKKKVELDDEASALDLVKEEVRLVEGPSMNMRIRTASD
                     FALAMALLKG"
     gene            3402..4118
                     /locus_tag="PK634_05880"
     CDS             3402..4118
                     /locus_tag="PK634_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97300.1"
                     /translation="MKRIGGAMCILAAAFLAGCTMARMAVPPDLPASASAMPVEGRKT
                     FSFDESFHFGGYRVESVRRGWTHSTAWRFLGFESGKAKQKYEFALKAAGGTSWKAHCA
                     TGVRWKEMEFENFLDSKGTLTWPLHSDTGFACTFEGGEGAPSWKLAMEQGADDLVLNG
                     VLTDGTAVIRVEGIRKLKGTPIPLGEPSGFLFLGPDGLVGAVEVINNGTVWIAGDVSP
                     ELQSAVACTSASLLLYRALK"
     gene            complement(4143..4691)
                     /locus_tag="PK634_05885"
     CDS             complement(4143..4691)
                     /locus_tag="PK634_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97301.1"
                     /translation="MPSRLSSRLGLAVLAAAVALYGALAWHHRDVSRISRRLDRLRKL
                     FNKEAGESPLTALRRAHEMAGAFTASPEIRLGPYLPDLFSRQELVVAVHRARLLLEEI
                     EVTIRDKLITVSDDRQSATTDLAAEGVYLYRGEQSRDITEIRLTWVKENGEWLIRSAV
                     PLETIQPPGKVRRMIQRGLANP"
     gene            complement(4691..5206)
                     /gene="lepB"
                     /locus_tag="PK634_05890"
     CDS             complement(4691..5206)
                     /gene="lepB"
                     /locus_tag="PK634_05890"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOW97302.1"
                     /translation="MNDESQPASLLKKFTVGENVKTTVRRAILLALFCLLLFNYVLLP
                     VRVRGISMEPTFHDGSWHVANLLQFATRGPRRGEVVVISMVGGRRTLYLKRVLGLPGE
                     TIHFFNGQMIVNGREETEPYLGDRGKWTLPQVTLAADEYFVAGDNRTAPLETHTLGVV
                     KRKKIAGGVFR"
     gene            complement(5203..6918)
                     /locus_tag="PK634_05895"
     CDS             complement(5203..6918)
                     /locus_tag="PK634_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOW97303.1"
                     /translation="MTPQLLPAPRRLRLLGGWLAGSAAPRLESGAGSPESYSLTVDRD
                     VRIAAADEVGLLHGQRTLRQLRHQYGARLPRLRIEDAPAFPNRGYSLDISRGRVPTLA
                     SLKTLVGKLSALKVNRLQLYIEHVFDFGFDPDIGAGSDPLTARDIRELDRHCRARHVE
                     LIPCLACFGHMGKALSLPRYRGLAEVEWPARDWESATWRQRLRGATLNPRRPESRRLI
                     RRMLDEFLPLFSSARFNMCGDETYDLGRGVNAGWVRRRGVAALYLEHLEFVRGVAREH
                     GKQLMFWGDVMQQHPESIRRVPRDCIALDWGYERHTDFEKAGRFIRAGIETWVCPSTR
                     GYKVVFNEVEEARANIAGYARTGRRLGASGLLVTDWGDMGHFNLPAASLHGLALGAAM
                     GWNPDSSEGAVFDRAWSIQALGDPAGKAAALFAQAGSAGWARWPLLLQGPDADPPPPE
                     AARLAARQAAACRRRTRRFAGLRPNGTLLSNDVEELAVAAEAMELTAQRLALGPGRAR
                     PGQKPALNRRRIVVFIRALESFFRRYARVWTKTSRPAGLKDLARAFRQAARSWREWAK
                     KEGTP"
     gene            6954..7259
                     /locus_tag="PK634_05900"
     CDS             6954..7259
                     /locus_tag="PK634_05900"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOW97304.1"
                     /translation="MKEQVQGEGTARRRVSVRYEGRVQGVGFRFTVVQVSEGFDVTGF
                     VRNEYDGSVGLVAEGPEAGLRDFLQAIHLSPVGRHIRGEQAVWSAAEGGFHGFGVKF"
     gene            7332..8054
                     /locus_tag="PK634_05905"
     CDS             7332..8054
                     /locus_tag="PK634_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOW97305.1"
                     /translation="MKYAILGDIHANLEALTAVLADAKEQGVTHYACVGDVVGYNANP
                     VECVNLIRELSPTVVRGNHDHYCSHDDNLSGFHPLAADAVDWTRKQLPEEMRLYLRKL
                     KLVAMIESFTLVHSTLDMPEMWGYVFDKLEAEAHFHYQTTAICFFGHTHVPLAFEKAD
                     GVRCGLYTRIQVTRGKKYFINVGSVGQPRDGDPRASYAIYDMIRNQVELRRVAYDFRI
                     TQKKILDAGLPGRVAARLAVGR"
     gene            8098..8958
                     /locus_tag="PK634_05910"
     CDS             8098..8958
                     /locus_tag="PK634_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97306.1"
                     /translation="MKQSGWLAGWIGLSLACAASGQVEVGCRLEASRALLFEPVRVTV
                     RVTNNSGHPLHFGGSSPDARLGFSIEQAPGEPVLTTGQPLLAAPLDLAPGASREVTIN
                     LLPSYRIHATGPYTIQARVDLGDEAYVSQKAYLDVLPGFEVGRLAVGLAGASKDTRIY
                     TIKTLTRDRMELLFLRVDDDKSGRCLGVFDLGRIVRQFQPQMAGDSRGHVHVLHQSSP
                     WKFTHTEINPDGIPVAARDFSAYSTEVRLEKEPDGSVIVRGVRPAPDPSLFQPPATLT
                     PEPAGGKKRR"
     gene            9008..9094
                     /locus_tag="PK634_05915"
     tRNA            9008..9094
                     /locus_tag="PK634_05915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9042..9044,aa:Leu,seq:cag)
     gene            complement(9589..10407)
                     /locus_tag="PK634_05920"
     CDS             complement(9589..10407)
                     /locus_tag="PK634_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOW97307.1"
                     /translation="MSGILESLQYAFVQRALLAGLFIALGCGLLGVFLVLRRYAMIGE
                     GLSHFAFGTVGLALLLGWTPLYVALPLVALASLVILYLPERAAMYGDAAIGMVSAVGV
                     AAGVLLSSLGGGFNVDLFSFLFGDILSVSRAETRLAVGLALGVVTLIALFYHELFAVT
                     FDEAGARVVGIRASRINRLLALLTALMVVLGIRVVGTMLVSSLLIFPAVTALQFRRGF
                     RATLVLAAVLGTTSVLAGLLLSLALDAPAGAAIVLVNAAAFAAAFLATRHVRAA"
     gene            complement(10404..11177)
                     /locus_tag="PK634_05925"
     CDS             complement(10404..11177)
                     /locus_tag="PK634_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOW97308.1"
                     /translation="MRVNPIEVNDLSASYGAGTALDHVSWRAGSGEFWAVVGPNGSGK
                     TTLLKTLLGLLPAAAGEIALFGTPTGRFGDWHRIGYLPQFSGIASPRFPATVREVVGM
                     ERLAAKRFPRWLGPADATAVTDSLARLGIADLAARRIGELSGGQRQRVLLARALVNRP
                     PLLLLDEPTLALDPDSRNAFYELLAEMNRRDGVTIVLVTHDSATAGNYASHLLYLDRK
                     VVFSGSFSDFCSSPEMTSRFGAFAQHTICHQHDRAGGAP"
     gene            complement(11168..12091)
                     /locus_tag="PK634_05930"
     CDS             complement(11168..12091)
                     /locus_tag="PK634_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOW97309.1"
                     /translation="MRNLAQWTAWMALACAAAIAARAEPVRVVTTIFPLYDWARAVGG
                     ERVDVAQLLPPGVEAHSYAPRPSDVFALNRADVFLYLGADLEPWAADLVRGIENPRVA
                     VIEAGREVPREHGHDEDDEEDGEHHHEASDPHIWLDPVLAQTVVSTIAAGLAQADPAG
                     AAEYAARAGRYNAQLAALDEDIRAGLANCRHRQILYGGHFAFGYFARRYGLTHLSPYA
                     GFSPNAAPTPAQMAELVKAIRASGQTTLFVEELLDPKVGRTLAEETGTRLEVLHGAHN
                     LTREERTRGDVTYLSLMRENLAKLRKALGCE"
     gene            complement(12166..13635)
                     /locus_tag="PK634_05935"
     CDS             complement(12166..13635)
                     /locus_tag="PK634_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOW97310.1"
                     /translation="MLQASCDVLLCTSNARYIHPAFGLRCLLANLGPLQSRARLLESD
                     IKTEPSTVAEAILAEAPRIVGLGAHVWNATRLADIAARLKAARPDLVLVLGGPELTSE
                     ETDLTRPADYIVVGEGEEVFRRLCVDLLEGRRPAGRFLRAAPVDLSRLALPYELYTDA
                     DLKHRITYVESSRGCPYHCDYCLSAGDAPVRYFPLPPLLDALDRLLDRGARTLKFVDR
                     TFNLDLERCCALLAFFLERMRPGLFVHFEMVPDRFPDELRGLLARFPTGSLQLEIGVQ
                     TFNPEVAARIRRPLRPELVEENLAFLRKRTNAILHTDLIAGLPGETAESFAAGFDRLA
                     RLDPHKIQIGILKRLPGAPLARHDGPGAMRYNPRPPYEILSTSAMKEAELIEIKRFAR
                     FWERVVNRGHFPRTAPLLWAGAASPYRAFAEFSRWLFARFGRDYRLPLTELADALFEF
                     LTEARGLSRERTATSLLNDYREPGRKDTPRGLRPYTARG"
     gene            13767..15245
                     /locus_tag="PK634_05940"
     CDS             13767..15245
                     /locus_tag="PK634_05940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97311.1"
                     /translation="MHNNHLGMSAFQRVALVNVRLESRQRIPVNLLVLAACIKDRTRV
                     TVFDPDPDQGDLRGLVEFQPDLVGISFMTQTALRAKELYDILRAALPSAHFVLGGVGP
                     TVEKEAVFERFRPDAVVVGEGEKTLRRIVDGEPLDQVPGVYRTGRPFTPGEVIWNLDE
                     IPLPAYECMPDFEKYLCPPGGIRGKWFDAGTPMIMTGRGCPYQCTFCSSHLMFTRKVR
                     RRSVDNVLQEIRRLRNEYGIEAVHFLDDTFNAPPSWARNFCEALLREPYRLTWGCQVR
                     VNLFDPDLARLMRKAGCVQVDIGVESGSPKVLKALKKGETPEQTAAAFRAAHEAGIAP
                     MATFLVGCPEETMEDIELTRQLVKRIKPSFSEFYFLIPFPGSELFEQATANGWLKDRS
                     YEGRGMVDRPVMEINFTMEQQVEIRRAYFQLVGWRNLAGFVSLNVLWAMMKSLRPSMV
                     AAALKEFWKTRNLRDAMQAYVHALRAAYGRRAAQQRSQRAAP"
     gene            15345..16616
                     /locus_tag="PK634_05945"
     CDS             15345..16616
                     /locus_tag="PK634_05945"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOW97312.1"
                     /translation="MKTATVSRGQPEVVGRPRWLAFLICGALALAALLALGAVTQMAG
                     SYSAANRLAAEGWGDGAQIIVRTRIAALWHGAFIALCAGAFFLLLSGRRAGWSFLQVA
                     MARWGLVLVMAADAILLARHYVHSMPRTAVQENEVVRLLKTGIPDKRVAMLTQEGFYN
                     NWLTYLFPYHGIKSVNITQMPRMPAEYERFLQAVGRHPLRFWQLSAVGYVMAPVGAWN
                     QIQADPNMKDAFAPALEYNVGLRDGDLVVVPATAYSPGQHVVLAFRKPAPYAALFAGW
                     AAANDDEALRRLADPAVEPFQVLQVAPETADDLPASQAQGLTGQARVLAYRSGRMRIG
                     ISADRPAILRVAEKFDPGWQARVDGRPAPVRRVDFLFMGVAVAPGDHEVELCYRSGAG
                     SLYVLGLGVLLCGGAALSLLVRRPAGEAAHG"
     gene            16609..18243
                     /locus_tag="PK634_05950"
     CDS             16609..18243
                     /locus_tag="PK634_05950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW97313.1"
                     /translation="MDRPSCGPGRALLKRLLLAVVMTALVLAALEGALRLAGFRHEPR
                     QKVLWKPTVAGFIGTEEFYIQTDFAPPGYIWVSQPNTPYTDRYGFRRPEVPVEKQPGQ
                     FRVAFLGGSTTQGGYRPYPERVIRLLNAATGGTRYEMLNVGCSSYSTHQSLIALERWV
                     WPRHPDLVIVYHGWNDQIVQADGFSDHEKDALLSLAPGSRPSWAVRVQNMRLAQGVGR
                     LLQAVDRSWPRPRVPVDRFRSNLDEFAERCRKRGTRLMIFVRPESRRYPLPAYEAGAL
                     EHFSRAYKTTNTLELYKAIHADYSAAQREPREGAEVFDAWAVVNRLYERQQAGEFGPG
                     VEIWQNDSIHLRPLGEELLAQELAPAIAPEAAEAVKTFVQSGRYWSFLAGEFLNERLP
                     HEAVYCARRAKEADPALTAAMDELTRRAEKDFEFVALFEEGRWGGTDPAFESKLRKLS
                     KCLGMRPADHGVLVQIFRTCLYSNRPEMAADALAGYRPASAPHRYEWLNMRLQSLLAG
                     NRLQEAEGVAVEMLNMNPREAQVRGVLDQIRARRTP"
     gene            18240..19490
                     /locus_tag="PK634_05955"
     CDS             18240..19490
                     /locus_tag="PK634_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97314.1"
                     /translation="MNGPLTDRQTDRMDRFLFVAILLLVAVAIFRSILLPGRFLFTTD
                     DNIGDLALRKALLPGGYSGGWYDGMLLGFNMVIPPGWTHLLLWLSPLIPFVNWMHGLD
                     LVLASLALAAFLRRKDVSWPACAVAALAAFWVGSNFTLTYAGHIMKFGVLMWAAAYLW
                     WVDRAVETGRPALAVLAGGALGAMFLEQADVALFFALALGPYALFRLGQTAGAQGRKW
                     AVVLVPLLLSALIIAFHPLWEGYRANVQGVTSMEQENPGEKWAFITQWSWPPEESIDF
                     VAPGYMGWRSGEPAGPYWGRMGRSEGWEQTRRGFQNFKLENQYLGAIPLLLALAALAG
                     ARQKPEIIFWGVAALVTLLLSFGKYFPLYAVFYKLPVINNIRNPNKFLQVFQLVLAIL
                     SAYGFDLVLKAGRKPDPIGAPPRA"
     gene            complement(19505..21058)
                     /locus_tag="PK634_05960"
     CDS             complement(19505..21058)
                     /locus_tag="PK634_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97315.1"
                     /translation="MNEPVSSPSRRALDALPYLALAAYIVMTFVLIPRHELWRDEAAD
                     WLYTRWGPSLAAVLHDLGYQTDPGGWHTALYFLLKVFPSPLAMQVFHSLMAIAGVFLF
                     IRFGPFTRLEKILYPFGFLVFYEYNIVARNYVPILLFLCLAATLYAQRFRRPWLYMLP
                     LALMAQTHMYGGLFAGTLALLYVIELAAAHRTRWREYTPFLAPLFALGLVFLVSLYQL
                     IPFAEVRAVLSGGGEIDYFQTARGVLLRLAAPVVKPQFYFWPANTVSDQHSTLLALLG
                     LAGLLYLVHAKPVPLGYFLVTGGIIAYLLVFRGSNMRFIGMLWLLLVFTQWIGRYYPA
                     PGRWADSRWMKRYPLLRAAIFSAFLALQAIGTVTAFRMDFQHPFSGGRETAQFLNAHL
                     KSTNVLVGTVLSYATQSIQPYMRPEFPPFYCIEQERFYQGLICDAQWYENDKKVPPVE
                     AIQRLIRKADSGTYDQVLLITLMPLQAPGVRVIGPFPLDRRVLCGDESFFVHEIDVDT
                     LRAQLEANR"
     gene            21182..23218
                     /locus_tag="PK634_05965"
     CDS             21182..23218
                     /locus_tag="PK634_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97316.1"
                     /translation="MAGNNNSVDEGRPAASGRTGLMLGLAVLLALSIGIRFYKLGVTD
                     TRSDEVELVEQLQGGVKPVEYFKWFIEVFQAGRQMPLSRVATCIYMRTFELPVTLFHV
                     RTPFAMLSALTPLFLVFLGRWWAGPRLGWILGVLGLVNPFALFWGRIGLAYGFVLPFV
                     VLTAAFFVPLLNNAAGGRPLPRGLLAGVTAAAVLGSYSHLSAWPFTGLLWLALFFLVL
                     KKAAWNFRAVPARCWIAFAVWALSIAPWAYSFLTAVNTKWDILDKISNDPVYTFAAMR
                     RLPFVMGWGRGVPGVFLTLGLPLLGLVLAWKNRGLRPGLALTLLIGVVLFAMLALMMT
                     LSGGIYTLRYFTPLWIIPILLAGLAIHEAAAWVGRRWPRPGAATLSAALFLAGVAAHA
                     AAPLWWTVNLTGHPTPYSHVSRWLDAHLPKGAPAIVDRWFEPSHEMRFHAPTNTYVTF
                     TVPNEPLKNFLELKWRDTVKQFALRYPDAAYVELIKCYFYVPTVGGWEWPRQYFARHA
                     VITNEPALKLRQRWLGMTEDYYADNTNRIVCEIFYNTTEDMLEKARAAGQPFFLQYGE
                     GWRYAKTQDFRDWRMLEGAAEVELFNLTGAPQEARLTVRGAAVEGTKQVRSSTGAQET
                     FPANQRHEWAVVLTSLPPGRTVVRLTDSLGPAAHAVLLVEDLRLAADQGASITR"
     gene            complement(23194..24603)
                     /locus_tag="PK634_05970"
     CDS             complement(23194..24603)
                     /locus_tag="PK634_05970"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW97317.1"
                     /translation="MQRRVRLFSLHALWIGLAIAPCARAITRYVALSGASIPPYTNWA
                     MAAATIQTALDASQDGDTILVSNGVYSTGSRVTPGAILPNRVVITNNVILQSLNGPTN
                     TVIAGDRGTPVRCVYASGNAAIIGFTLTNGTARATGSDKDTWGGGLFFNGASDGLVSN
                     CIVSGNFALNGQGGGISLYNANVCVCDCVIDGNSADGGGGMRLQAGLPTLRNLVLTHN
                     ESDGGGGAIHYYHASGTARNLLIAGNSSITQGGGLYFDGSDAELDSVTIAANSAPSGY
                     GGGVMVSYDSSPSLHNCILWDNTTEQIAFNPEWYDIEITLSYSDVQGGEAGIVTHGVG
                     PVNWLAGNTNINPLFEADGSYRLQNLSPCVDVGENMAWMPDATDLAHNPRIVNGLVDM
                     GAFEYGVDRDISIRVSAVDVVWFGYVGETYQVQGAMSLLNPVWENIGSPVAGTGAYMY
                     VMDVTRDTGHRYYRVIEAP"
     gene            complement(24667..25347)
                     /locus_tag="PK634_05975"
     CDS             complement(24667..25347)
                     /locus_tag="PK634_05975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOW97318.1"
                     /translation="MEWGRKIKRSIIRCGIPPSSSLDFFNSLLALGRWMRAHPAPRHF
                     ERRLQLFEHVAGLMGGEPFDYLEAGVMFGDSIREWSQLSAHPETRFYGFDTFTGLPEA
                     WQTGLKKFEPGSFSNDGQVPRVDDARVTFIKGLFQDTLPGFLAEYKPQKRLIVHCDAD
                     LYTSTLYWLCTLNGLLKTGTILLFDDFAVPTHDFRAFLDYTGAFRKPYKVLATAEVDF
                     EKIAFELT"
     gene            complement(25448..>25675)
                     /locus_tag="PK634_05980"
     CDS             complement(25448..>25675)
                     /locus_tag="PK634_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97319.1"
                     /translation="LQRILEKHGFAVRRREGSYIFPFENSRLGVWVARRVPRWGAQVF
                     VAAEKQRDVRIEEGFHPDMPALLKWLKGEEA"
BASE COUNT         4292 a         8351 c         8773 g         4259 t
ORIGIN      
        1 gaaccggcgg caagttcctg gaaccggtgg acccgctgga cctgggcaat tccggcaccg
       61 ggatgcggct gctcgccggg ttgctcgccg gcctgccgat cacggccgag ttgacgggcg
      121 acgagtccct ccgttcgcgt cccatgaacc gcatcaagga gcccctggaa aaaatggggg
      181 ccgtcgtaga gctgctcggc gaaaagggcc gcccgccgat ccgtatacgc ggcggcaatc
      241 tgaagggcat ggactatgcc atccccgtcg ccagcgcgca ggtgaagtca tgcatcctgc
      301 tggcgacgct cttcgcggag gggacgacga ccgtcacgga agtcctgccc acgcgggatc
      361 acaccgagcg gctcttcctc aacgcgggga tccaactgga agtggacggc cttcgcgtgc
      421 ggatgaaggg ctatggcgcc aagggcccca agctcaaggc gcgcccctgg gaggtgcccg
      481 gcgacttctc gtcggccgcg tatgccctgg ccgccgtcgc ggcccggccg gattcctcgg
      541 tcaccgtgag cggggtcggg ttgaatcccc gccgcaccgc gctgctggac gtgctccggc
      601 gcatgaatgc ccgcgtcgag gtggccacgc gcagcgggac cgacgaggcc gagccgttcg
      661 gcgacgtaac cgtttccggc gcccggctca caggcacgac cgtcggcggg ggggagatcc
      721 ccgcgctcat cgacgagttg ccgctcgtcg ccatcctcgg ggccctggcc gagggcgaga
      781 cggtcatccg cgacgcccgc gaactccgag tcaaggaatc cgaccgcatc acctgcatgg
      841 ccaacaacct gaagctgctc ggcgtggacg tcgaggagcg cgaggacggc atgctcatcc
      901 gcggcccggc ccgcctgcag cccgtggccg gcatccagag ctacggcgat catcgcatcg
      961 ccatgtcgct ggccgtgctc ggactctacg gcgaggggcc ggtgtgcatt cacaacatcg
     1021 cttgcgtgtt aacatcctat cccgcctttt gggatcatct cattgcattg ggggcacatg
     1081 tcgaataacc attccgtcag cgtcgtggcc atcgatgggc cgtccgcgtc cggcaagtcc
     1141 accgtgtcca agcgggtcgc ccaggaattg aaattcacct acgtggattc cggctcgctc
     1201 taccggggca tgacctggaa ggccctgcgc gagagcgtca acatcggcca gcccgagcag
     1261 gtgctcgacc tgatgaaccg gatgaagctc gaattctacg ccgagaaccg ggtcgtgctg
     1321 ttcaccatcg acggggagga tcccggcctg caactccggt ccgagccggt ccgggaaaac
     1381 gtggccgaca ttgccgccat ccccggggtg cgcgcattcc tggtggcccg gctgcaggag
     1441 atggtccggt tcggcgacat cgtcatggaa ggccgcgaca tcggcaccgt ggtgttcccg
     1501 gaatcgccgt tcaagtacta cctcgatgcc gaccccgagg agcgggcccg ccgccggagc
     1561 ctcgaactca agaacatgga gggcgtgggg gacgtcagca aggtgctcga ttcgctgacc
     1621 cgccgggaca agaaggacag cacccgtaag accgctcccc tgcagatcgc gctcggggcc
     1681 aaggtgatca actccacctc catgacgatc gaggaggtcg tcgcgcagat cgtcgccgag
     1741 gttcgggccg ccctgggaac atgaaagacc gttgcctgtt gtaccggttt gtccgcggga
     1801 gcacggcgat ctggctcaag gtctggctca agctggaatc ccatggcgcg gaaaacgtgc
     1861 ccgccgccgg cggatgcgtg ctggcctcta accacgcgag ctatctcgat cccccgatcg
     1921 tcgcctgcgg tttgaagcac cggatcgtgc gcttcatggc ccgcgacacg ctgttcgccg
     1981 ggacgttccg aaactggttc ttccacggga tccggtgcgt gccgttggac cggactcgcg
     2041 gcgatgtggc ggccttgcgc aaggggatcc agatcttgaa ggagggagaa gtgctgggcc
     2101 tgtttcccga aggcacgagg tcgccggacg gccaattgaa acccgccaag ggcgggatcg
     2161 gcttccttgt cgccaaggcg ggcgtgccgg tggtgccggt ttacgttgga ggcagcttcg
     2221 aggcgttccc gaagggcgca cagcgcatcc gtcgcggcaa ggtctttatt tactacggcc
     2281 agccgatagc ctttgaggaa attgcgcggc tgggacagga cagggattcc taccaggcca
     2341 tcggcgatct gatcatgtcc aaaatcgcgc atcttcgaga acaggctctc gtttccccgc
     2401 gctgaacggg acattctgtc tcctcgcaac ctgatgcggc gcggaaactg ataaaatcga
     2461 gttgcatatt tgctttataa tgaagatgat ataaaatcac cgcaaggcgt gataggggga
     2521 aatacgtagg agtgtaagaa aacagcatcc ataaaccgtt gaaatcaaaa ggattcttga
     2581 catcacacag catgtcggta aacatttcca cgaattagcc gcgcgtctct ggcgtggcag
     2641 ggttgttctg aacatcgaat cgggaatgga ccgatgatct acacgtgttg gggtgttgta
     2701 ttggcctgcg ggaaggatga gcaattcacc cctgaaattt ccactccttt catcaacctt
     2761 tcaggaaaac cgatcctcgt tcatgcgctg accgcattcg agcaatgcgt ggacatttca
     2821 tacgtccttg tcgtagccga gaaaacgcgc atcgacagtg ttctgggcat ggcccagatg
     2881 tacggtttgt caaaagtgaa aaaggtgatc gcgggttttg gctcgcgcca ggcgtccgtg
     2941 caggccgcgc tcaagaatct ggacgaggag gcgaacctga ttgtcgtgca cgagggttca
     3001 cgcccgttcg tcaaacccga gttgatcagc gaggtcatca agagcacgaa gcgctatggc
     3061 tccggcgtgg cggccgtgcc gctggccgat gccgtcaagg aaacccgcaa ggggctgacc
     3121 gtggccacct ccgtgacggg cggcgcgtac tggctcgcgc agacgccggc ggcgttccgg
     3181 cgcgacctgc tggaaaaggg cttgaaggcc gcggcgaaga agaaggtgga gttggacgac
     3241 gaggcatccg ccctcgacct ggtcaaggag gaggtgcgcc tggtggaagg cccctccatg
     3301 aacatgcgga tccgcacggc ttccgatttc gccctggcga tggccctctt gaaagggtga
     3361 cgcgggctcc tctccgcggc gcccaggcac aggagcattc catgaaacga atcggcgggg
     3421 ccatgtgtat cctggcggcg gccttcctgg cggggtgcac catggcgcgc atggccgtgc
     3481 cgcccgacct gccggcctcg gcgtccgcca tgcccgtgga aggacgcaag accttcagct
     3541 tcgacgaatc gtttcatttc ggcggctacc gggtggaatc ggtgcggagg ggttggaccc
     3601 attccacggc ctggcggttc ctcggcttcg agagcgggaa ggccaagcag aagtacgagt
     3661 tcgcgctgaa ggcggccggc gggacttcct ggaaggcgca ttgcgccacg ggcgtccggt
     3721 ggaaggagat ggagttcgag aacttcctgg acagcaaggg caccctgacg tggcccctgc
     3781 attcggatac ggggttcgcc tgcaccttcg agggcggcga gggcgcgccg tcctggaagc
     3841 tggcgatgga gcagggcgcc gatgacctgg tcctgaacgg cgttctcacg gacggaacgg
     3901 ccgtcatccg ggtcgagggc atccggaaac tcaagggcac tccgatcccg ctgggcgagc
     3961 cttccggctt tctcttcctc ggtcccgacg gcctggtggg cgcggtggaa gtgatcaaca
     4021 acggcaccgt ctggatcgcc ggcgacgtct cgccggagtt gcagtcggcg gtggcctgca
     4081 cgtcggcctc cctgctgctc taccgcgccc tgaagtaatg ccgcccgggc cgctgatccg
     4141 gcctacgggt tggccaggcc gcgctggatc atccggcgca ccttgcccgg aggctggatg
     4201 gtctccagcg gcacggccga gcggatcagc cactcgccgt tctccttgac ccacgtgagg
     4261 cggatctccg tgatgtcgcg gctctgttcg ccccggtaga gatagacgcc ttccgccgcc
     4321 aggtccgtgg tggcggattg ccggtcgtcg gacaccgtga tcagcttgtc gcggatggtg
     4381 acctcgattt cctccaggag caggcgggcg cgatgcacgg ccacgaccag ttcctgccgg
     4441 ctgaacaggt cgggcaggta cggccccagg cggatctcgg gcgaagcggt gaaggcgccg
     4501 gccatttcgt gggcgcggcg cagcgcggtc aggggcgact cgccggcctc cttgttgaac
     4561 agcttccgca ggcgatccag gcgccggctg atgcggctga cgtcgcgatg gtgccaggcc
     4621 agcgcgccgt acagcgccac ggccgcggcc aggacggcca gccccaggcg cgatgagagg
     4681 cgcgaaggca tcaacggaag accccgccgg cgatcttctt ccgcttgacg acgcccaaag
     4741 tatgggtttc cagcggggcg gtccggttgt cgccggccac gaaatactcg tcggccgcca
     4801 gcgtgacctg gggcagcgtc cacttgccgc ggtcgcccag gtaaggctcc gtctcctcgc
     4861 ggccgtttac gatcatctgg ccgttgaaga agtggatcgt ttcccccggc agcccgagca
     4921 cccgcttgag atagagggtg cggcggccgc cgaccatcga gatcaccacc acctcgcctc
     4981 gccggggccc ccgggtcgcg aattgaagca ggttggccac gtgccaggag ccgtcgtgga
     5041 acgtgggctc catgctgatg ccccgcacgc gcacgggcaa caggacgtag ttgaagagca
     5101 ggaggcagaa cagcgcgagc aggatcgccc ggcgcacggt ggttttgacg ttctcgccga
     5161 cggtgaactt cttcagcaaa gacgccggct gactctcatc gttcatggtg tgccttcttt
     5221 cttcgcccat tcgcgccatg atcgggccgc ctgccggaag gcccgggcca aatccttcag
     5281 cccggccggc cgggaggtct tcgtccagac ccgcgcatac cgtcggaaaa aagactccag
     5341 cgcgcggata aatacaacaa tccgccgccg gtttaaagcc ggtttttggc ccggccgggc
     5401 gcggccgggg cccagggcca gccgctgcgc ggtcagctcc atggcttcgg ccgcgaccgc
     5461 gagttcctcg acgtcgttcg agagcagcgt gccgttcggg cggaggccgg cgaatcggcg
     5521 ggtccgccgg cggcaggccg cggcctgccg ggccgcgagc cgggcggctt ccggcggcgg
     5581 cgggtccgcg tccgggccct gcaggagaag cggccaacgg gcccagccgg cggagcccgc
     5641 ctgcgcgaag agggccgcgg ccttcccggc gggatcgccg agggcctgga tggaccatgc
     5701 gcggtcgaac acggcaccct cgctgctgtc cgggttccag cccatggccg cgccgagggc
     5761 caggccgtgc agcgaggcgg cgggcaggtt gaaatggccc atgtcgcccc agtcggtcac
     5821 caggaggccc gatgcgccga gccgccggcc cgtcctcgcg tacccggcaa tattggcgcg
     5881 ggcttcctcc acctcgttga acacgacctt gtacccgcgc gtcgaggggc acacccaggt
     5941 ctcgatcccc gcccggatga accggccggc cttttcgaaa tccgtgtgcc gctcgtagcc
     6001 ccagtccagc gcgatgcaat cccgggggac gcggcggatg gattccgggt gctgctgcat
     6061 cacgtcgccc cagaacatca gctgcttgcc gtgctcccgg gcgacgcccc ggacgaattc
     6121 caggtgctcg agatacagcg ccgcgacgcc gcgccggcgg acccagccgg cgttcacgcc
     6181 gcgccccagg tcgtaggttt cgtccccgca catgttgaag cgggcggagg agaagagggg
     6241 caggaactca tccagcatgc gccggatcag ccggcggctt tccggccggc ggggattcag
     6301 cgtcgcgccc cgcagccgct gacgccaggt cgcgctctcc cagtcgcgcg ccggccattc
     6361 gacctctgcg aggccccggt agcgcggcag gctcaaggcc ttgcccatgt gcccgaagca
     6421 ggccaggcag gggatcagtt cgacgtggcg ggcgcggcag tgccggtcca gttcccggat
     6481 atcccgcgcg gtcagcggat cggagcccgc cccgatgtcc gggtcgaacc cgaaatcgaa
     6541 cacgtgctcg atgtacaact gcagtcggtt caccttcagg gcggacagct tcccgacgag
     6601 cgtcttcagc gaagccagtg tcgggacccg gccgcggctg atgtccagcg agtagccccg
     6661 gttcgggaag gcgggcgcgt cctcgatccg gagccggggc agcctcgccc cgtactggtg
     6721 gcggagttga cggagcgtgc gctgcccgtg gagcaggccg acctcgtcgg cggccgcgat
     6781 ccggacgtcg cggtcgacgg tcagcgagta gctctcgggc gagccggcgc cggattccag
     6841 ccgcggcgcg gcgctcccgg cgagccatcc gccgaggagg cgcaggcgac ggggcgcggg
     6901 aagtaattgc ggtgtcatcg cgtcgggtct agtataaacg ggtaacacgg cttatgaaag
     6961 agcaagtcca aggggagggg acggctcgcc ggcgggtgag cgtgcgctac gaggggcggg
     7021 tccagggggt cggcttccgt ttcacggtcg tgcaggtcag cgagggattc gacgtcacgg
     7081 gctttgtccg caacgagtat gacggctcgg tgggcctggt ggccgagggg ccggaagccg
     7141 gcctgcgcga tttcctgcag gccatacatc tttcgccggt gggccggcac atccgcggcg
     7201 agcaggcggt atggtcggcc gccgagggcg ggttccatgg attcggggtg aagttctgac
     7261 gaaggcggct ttacattggg cgaagacccg ctatgcttgc gtgaattcgc ggtgaaagcc
     7321 gcggtcggac catgaaatac gcgatcctcg gagacattca tgccaacctc gaggcgctga
     7381 ccgccgtcct ggccgatgcc aaggagcagg gggtcacgca ctacgcctgc gtgggcgacg
     7441 tggtcggcta taacgccaac cccgtcgaat gcgtcaacct gatccgcgag ttgagcccga
     7501 ccgtcgtccg gggcaatcat gatcattact gctcccacga cgacaacctg agcgggttcc
     7561 atccgctggc agccgacgcg gtcgattgga cccgcaagca actgcccgag gagatgcggc
     7621 tgtacctgcg gaagctcaag ctggtggcca tgatcgagtc gttcaccctg gtccacagca
     7681 ccctggacat gccggagatg tggggctacg tcttcgacaa gctggaggcc gaggcgcatt
     7741 tccactacca gaccacggcc atctgcttct tcgggcacac ccacgtgccc ctcgcgttcg
     7801 agaaggccga cggcgtgcgc tgcgggctct acacccgcat ccaggtcacg cgcgggaaga
     7861 aatatttcat caacgtcggc agcgtgggcc agccccgcga cggggacccg cgagcttcct
     7921 acgcgatcta cgacatgatt cggaaccagg tcgaacttcg ccgcgtggcg tacgatttcc
     7981 ggatcacgca gaagaaaatc ctcgatgccg gattgcccgg acgcgtggcg gcccgcctgg
     8041 ccgtgggccg ctgaaacggc gacgacccgg aatccgtggg ccgggggaga ctacagcatg
     8101 aagcaaagcg ggtggcttgc gggatggatc ggcctgtcgc tggcctgcgc ggcatccggc
     8161 caggtggagg tgggatgccg cctcgaggcc tcgcgcgcgc tgctgttcga gcccgtccgg
     8221 gtcaccgtgc gcgtgacgaa caacagcggc caccccctcc atttcggcgg ctcaagcccg
     8281 gacgcgcgat tgggattcag cattgaacag gctccgggcg agcctgtgtt gacgacggga
     8341 cagcccctcc tggcggcgcc cctcgacctg gccccggggg cgtcccggga agtgacgatc
     8401 aacctgctgc catcgtaccg gattcacgcc acgggaccct ataccatcca ggcccgcgtc
     8461 gacctgggcg atgaggccta cgtctcgcag aaggcctacc tggatgtgct gcccggcttc
     8521 gaggtcgggc gcctggccgt gggactggcc ggcgcctcca aggacacgcg catctacacg
     8581 atcaagacgc tgacccgcga ccggatggag cttctcttcc tccgggtgga cgacgataag
     8641 agcgggcgct gcctgggcgt gttcgacctg ggccggatcg tgcgccagtt ccagccgcag
     8701 atggccggcg acagccgcgg ccacgtgcac gtgctgcacc agtccagccc ctggaagttc
     8761 acgcacacgg agatcaatcc cgacggcata ccggtcgccg ccagggattt ctcggcctac
     8821 agcaccgagg tccgcctgga gaaagagccc gatggctcgg tcattgtccg cggcgtgcgg
     8881 cccgcgccgg atccttcgct gttccagccc ccggcgacgc tgacccccga gccggccggc
     8941 gggaaaaaaa ggcgatgacg ccacgctcgg tgtgtggtat atatttcctc gtagccaaac
     9001 gcccggtgca cccgtggtga aattggcata cacgcaagat tcaggttctt gtgccagcga
     9061 tggcgtggag gttcgactcc tctcgggtgc accatcttct cttccctgat cggtcgcccg
     9121 cgcacgggcg cgccgcccgc cgcgggcggc agcgcggggg ccatcgcggg gcggggcgcg
     9181 ggcatcgccg ccgccaggaa ctggcggcgg aacgactcct gctgctcggg cgtcatcctc
     9241 ttccagttgt aatcgaggat cgaccatagg atgcttcccg cgcacgccag ttgcttctgc
     9301 tccgccggaa gcatgggaaa gcgctgcgcc aggtccgtgg tgaacgcgcg ctggagttcc
     9361 ggcgccgtca ccggcccgtt cagattcgat gtccggcccg actcctcggc cagaatccag
     9421 ggcgcctcgg cggcgcgggc gtggaggcct gaaggcagga cggccgccac ggccgcgatg
     9481 catagccgga aaacatgctg gttcattcag cctccccggt tgctgtcggt caaggatacc
     9541 cgctttcccg cctggagaac agggctccgg gcgggcggca tccggccctc atgccgctcg
     9601 gacatggcgg gttgccagga acgccgccgc gaacgccgcc gcgttgacga gcacgatcgc
     9661 ggcccccgcg ggcgcgtcca gcgcgagcga gagcagcagt ccggccagca ccgaggttgt
     9721 gccgaggacc gccgcgagga ccagcgttgc ccggaacccg cggcggaatt gaagggccgt
     9781 gacggcggga aagatcagca ggctggagac cagcatcgtg cccaccaccc ggatgccgag
     9841 caccaccatc aacgccgtca gcagcgccag caggcggttg atgcgcgagg cgcggatgcc
     9901 cacgacccgc gccccggcct cgtcgaacgt gacggcgaac agctcgtgat agaacagggc
     9961 gatgagggtg acgacgccga gggccagtcc gacggccagg cgcgtttccg cccggctgac
    10021 ggacaggatg tcgccgaaca ggaagctgaa caggtccacg ttgaaaccgc cgcccaggct
    10081 ggaaagcagg acgccggcgg ccacgccgac cgccgacacc atgccgatgg cggcatcgcc
    10141 gtacatcgcc gcgcgctcgg gcaggtagag gatgaccagc gacgccagcg ccacgagcgg
    10201 gagggcgacg tacagcggcg tccatcccag caggagcgcg aggcccaccg tcccgaacgc
    10261 gaagtgggaa aggccctccc cgatcatcgc gtagcgccgg aggacgagga acacgccgag
    10321 caggccgcag cccagggcga tgaacaggcc agcgagcagc gcgcgctgca cgaaggcgta
    10381 ctggaggctt tccaggatac cgctcaaggc gcgcctcccg cccggtcgtg ctggtggcag
    10441 atcgtgtgct gggcgaaggc cccgaaccgg gatgtcattt ccggcgaact gcagaaatcg
    10501 gagaacgaac cggaaaagac gaccttccgg tcgagataga gcaggtggct ggcgtagttg
    10561 ccggccgtgg cgctgtcgtg cgtgacgagg acaatggtca cgccatcccg gcgattcatc
    10621 tccgccagca gttcgtaaaa ggcgttgcgg ctgtcggggt cgagcgccag ggtcggctcg
    10681 tccagcagga gcaagggcgg gcggttcacg agcgcgcggg cgaggagcac ccgctggcgc
    10741 tgtcccccgg aaagctcgcc gatgcgacgg gcggcgaggt ccgcgatgcc caggcgggcc
    10801 aggctgtccg tcacggccgt ggcgtccgca gggcccagcc agcggggaaa ccgcttggcg
    10861 gccagccgct ccatgccgac gacctcgcgc acggtggcgg gaaagcgggg ggacgcgatg
    10921 ccgctgaact ggggcaggta accgatccga tgccagtcgc cgaaacggcc ggtgggcgtc
    10981 ccgaacaggg cgatctcgcc ggccgccgcc gggagcagcc ccagcagcgt cttcagcagg
    11041 gtcgtcttgc ccgagccgtt gggtccgacg accgcccaga actcgccgct tcccgcccgc
    11101 caggacacgt ggtccagcgc cgtgccggcg ccgtaggacg cggaaaggtc gttgacctcg
    11161 atcgggttca ctcgcatccc agggccttcc gcagcttggc gagattttcc cgcatcagcg
    11221 agaggtacgt cacgtcgccg cgcgtgcgct cctcgcgggt caggttatgg gccccgtgca
    11281 gcacctccag ccgggtgccc gtttcctccg cgagggtccg gccgaccttc ggatccagca
    11341 gttcctcgac aaacagcgtg gtctggcccg aggcccggat ggccttgacc aactccgcca
    11401 tctgcgccgg ggttggcgcc gcgttcggcg agaagccggc gtacggcgag agatgcgtca
    11461 gcccgtaccg ccgggcgaag tagccgaacg cgaaatgccc gccgtacagt atctgccggt
    11521 gccggcagtt cgcgaggccg gcccggatgt cctcgtccag agccgcgagt tgggcgttgt
    11581 accgaccggc gcgcgcggcg tactccgccg ccccggccgg atcggcctgc gccaggcccg
    11641 cggcgatcgt ggacaccacg gtctgcgcca gcacggggtc gagccagatg tgcgggtccg
    11701 aagcctcgtg atggtgttcg ccatcctctt cgtcgtcctc gtcgtgtcca tgctcgcggg
    11761 gcacctcccg gccggcctcg atcacggcaa cgcgggggtt ctcaatcccg cggaccagat
    11821 cggcggccca cggctcgaga tcggcgccga ggtagaggaa gacatccgcc cggttcagcg
    11881 cgaagacgtc cgagggcctc ggcgcgtagg agtgcgcctc cacgcccggc gggagcagtt
    11941 gcgctacgtc gacccgctcc ccgccgaccg cgcgggccca gtcataaagg ggaaaaatgg
    12001 tggtcacaac gcggaccggt tcggcccgcg ccgcgatcgc tgcagcgcag gcgagggcca
    12061 tccatgccgt ccattgggcc aggttcctca tgtgtccgtc catgttattc aaaatgcgcc
    12121 gaaaatgaag ccggaatcga ttcgactaac ccacggattt gcggatcagc cgcgcgcggt
    12181 atacggcctt aatccccggg gcgtgtcctt gcgccccggc tcccggtagt cgttcaagag
    12241 gctcgtcgcg gtccgctctc gggacagccc gcgcgcttcc gtcaggaact cgaacagcgc
    12301 gtcggccaac tccgtcagcg gcagcctgta gtcgcggccg aaccgcgcga acagccatcg
    12361 gctgaattcc gcgaaggccc tgtacgggct ggcggccccg gcccagagga gcggcgccgt
    12421 ccggggaaaa tgcccgcggt tgaccacccg ctcccagaag cgggcgaagc gtttgatttc
    12481 aatcagttcc gcttccttca tggcggacgt ggagaggatc tcgtacggcg ggcgcggatt
    12541 gtaccgcatc gccccgggcc cgtcgtgccg cgcgaggggc gcgccgggca gccgcttcag
    12601 gatgccgatc tggatcttgt gcggatcgag ccgcgccagc cggtcgaatc ccgcggcgaa
    12661 actctccgcc gtctcgcccg gcagcccggc gatgaggtcg gtgtggagga tggcgttcgt
    12721 gcgcttccgc aggaaggcca ggttttcttc cacgagttcg ggccgcagcg ggcggcggat
    12781 gcgcgcggcc acctccgggt tgaaagtctg gacgccgatt tcaagctgga gcgagcccgt
    12841 ggggaagcgc gccaggaggc cgcgcaattc atccggaaac cggtcgggca ccatctcgaa
    12901 atggacgaag aggcccggtc gcatccgctc gagaaagaac gccagcaacg cgcagcaccg
    12961 ttccaggtcg aggttgaacg tgcggtccac gaacttgagc gttcgcgcgc cgcgatcgag
    13021 cagccggtcc agggcgtcca gcagcggcgg caggggaaag tagcggacgg gagcgtcccc
    13081 ggccgaaagg cagtagtcgc agtgataggg gcagccccgg gacgactcga cgtaggtgat
    13141 gcggtgcttc aggtcggcgt cggtgtagag ctcatagggc agggcgagcc ttgacaggtc
    13201 gactggcgcg gcccggagga accggccggc gggtcgccgg ccctccagca agtccacgca
    13261 caaccggcga aaaacctctt ctccctcgcc gacaacgatg taatcggccg gacgagtcag
    13321 gtccgtctct tccgacgtca gttccggccc gccgagcacg aggaccaggt caggccgggc
    13381 ggccttcaat cgcgcggcga tgtcggcgag gcgcgtcgca ttccagacgt gcgcgccgag
    13441 gcctacgatc cggggcgcct cggccaggat ggcctccgcg acggttgaag gctccgtttt
    13501 gatatccgat tccagcaggc gggcccggga ttggaggggg cccaggttgg ccagcaggca
    13561 gcgcaacccg aacgccgggt gaatgtacct ggcattcgaa gtgcacagaa gaacatcgca
    13621 ggaagcctga agcatggggg cactatagct gaacgaagcc ggccggacca tcctgccgcc
    13681 ccgcggcccg tcgcgctgca gggagcctgc gccgctccgg atcgccccgc cgccgttgcg
    13741 gattggcttg ccgcggagtc ccggccatgc acaataacca ccttggcatg agcgcgtttc
    13801 aacgagtggc cctggtcaac gtacggctgg aatcgcgcca gcggatcccg gtgaatctgc
    13861 tggtgctggc cgcctgcatc aaggatcgga cccgcgtcac ggtgttcgat cctgacccgg
    13921 accagggcga cttgcgcggc ctcgtggagt tccaacccga cctggtcggc atcagcttca
    13981 tgacccagac ggctttgcgg gcaaaggaat tgtatgacat cctgcgcgcc gccctgccgt
    14041 ccgcgcattt cgttctcggc ggcgtcggcc cgaccgtgga gaaggaggcc gttttcgagc
    14101 gcttccgccc ggacgccgta gtggtcgggg aaggagagaa gaccctgcgc cggatcgtgg
    14161 acggcgaacc gctggatcag gtcccgggcg tctaccggac gggccggccg ttcacgccgg
    14221 gtgaggtgat ctggaacctc gacgagatcc cgctgcccgc ctacgagtgc atgccggatt
    14281 tcgagaagta cctctgcccg ccgggcggca tccgcggcaa gtggttcgac gcgggcacgc
    14341 cgatgatcat gaccggccgc ggctgcccgt accagtgcac cttctgctcc tcgcacctga
    14401 tgttcacgcg caaggtgcgg cgccggtcgg tggataacgt cctgcaggaa atccggcgcc
    14461 tgcggaacga gtacggcatc gaggccgtcc atttcctgga cgacaccttc aacgccccgc
    14521 cgtcctgggc ccggaatttc tgcgaggcgc tgctccggga gccctaccgt ctcacgtggg
    14581 gctgccaggt ccgcgtgaac ctgttcgacc cggacctggc ccgcctgatg aggaaggccg
    14641 gctgcgtcca ggtggacatc ggggtggagt ccggctcgcc gaaggtgctc aaggccctga
    14701 aaaagggcga gaccccggag cagacggccg ccgctttccg cgccgcccac gaggccggca
    14761 tcgcgccgat ggccacgttc ctcgtaggct gcccggagga gacgatggag gacatcgaac
    14821 tcacccggca actggtcaaa aggatcaagc cgtcgttttc cgaattctac tttctcatcc
    14881 cgttccccgg ctcggagctg ttcgagcagg ccacggcgaa cggctggctg aaagaccggt
    14941 cctacgaggg gcgcggcatg gtcgaccggc cggtcatgga gatcaacttc acgatggagc
    15001 agcaggtcga gatccggcgg gcctacttcc agttggtggg atggcgcaac ctggcggggt
    15061 tcgtgtcgct caacgtgctg tgggccatga tgaaatccct gcggccctcg atggtcgcgg
    15121 cggccctgaa ggagttctgg aagacccgca acctgcgcga cgccatgcag gcctatgtgc
    15181 atgcgctgcg ggcggcttat gggcggcggg cggcgcagca gcggagccag cgcgccgcgc
    15241 cgtaaaccgg tcagcctatt ctgcccgacc gtccagggca acaaacgatc gaggagcttt
    15301 aataagttga agaacatccg gccgttcctt aagaatagca ccgcatgaaa acagcgaccg
    15361 tgtcccgagg ccagccggaa gtcgtcgggc gcccccgttg gctggcattc ctgatctgcg
    15421 gcgcgctggc cctggcggcg ctcctggccc tcggggccgt cacccagatg gccggctcgt
    15481 attcggcggc taaccggctg gccgcggaag ggtggggcga cggcgcccag atcatcgtgc
    15541 gcacccggat cgccgcgctg tggcatggcg cgtttatcgc gctttgcgcg ggggcctttt
    15601 tcctgctcct gtccggacgc cgggccggct ggtctttcct tcaggtcgcg atggcgcggt
    15661 gggggttggt cctggtcatg gcggcggatg cgatcctgct ggcgcgccac tacgtgcact
    15721 ccatgccgcg gaccgccgtg caggaaaacg aggtggtccg gctgctcaag acgggcatcc
    15781 cggacaagcg ggtggccatg ctcacccagg aaggcttcta taacaactgg ctgacctacc
    15841 tctttcccta ccacggcatc aagtcggtca acatcaccca gatgccgcgc atgccggcgg
    15901 aatacgagcg gttcctgcag gcggtcgggc gccatcccct gcgtttctgg cagctgtccg
    15961 cggtcggcta cgtcatggcc cccgtcggcg cttggaatca gatccaggcc gatccgaaca
    16021 tgaaggacgc cttcgcgccg gccctggagt acaacgtggg cttgcgcgac ggcgatcttg
    16081 tcgtggtccc ggcgaccgcg tactcgcccg gccagcatgt cgtcctggct tttcggaaac
    16141 ccgcgccgta cgcggccctt tttgcggggt gggccgccgc gaatgacgac gaggcgctgc
    16201 gccgcctggc cgatccggca gtggaacctt ttcaggtgtt gcaggtggct cctgaaaccg
    16261 cggacgacct gccggcatcg caagcgcagg gactgaccgg gcaggcgcgc gtcctcgcct
    16321 accggtcggg ccgcatgagg atcgggattt cggcggaccg gccggccatc ctgcgcgtgg
    16381 ccgagaagtt cgatccgggc tggcaggctc gcgtggatgg gcggcccgcg ccggtgcggc
    16441 gggtggattt cctgttcatg ggcgtggccg tggcgcccgg cgatcatgaa gtcgagctgt
    16501 gctatcgctc cggcgcgggc agcttgtacg tcctgggcct gggggtgctc ctgtgcggcg
    16561 gcgccgcgtt gagcctgctg gttcgtcgtc cggccgggga ggccgcccat ggataggccg
    16621 tcctgcggtc cgggccgcgc cctgctcaaa cggctcctgc tcgccgtggt catgacggcg
    16681 ctggtgctgg cggccctcga gggagccttg cgcctggccg ggtttcgcca tgaaccccgg
    16741 cagaaggtgc tctggaaacc cacggtggcc ggcttcatcg gcacggaaga gttctatatc
    16801 cagaccgatt tcgctccgcc cggctatatc tgggtgtcgc agccgaatac cccgtacacg
    16861 gaccgctacg gcttccgccg ccccgaggtc cccgtcgaga agcaacccgg gcaattccgc
    16921 gtggcgttcc tggggggctc caccacgcag ggcgggtacc ggccgtatcc cgagcgggtg
    16981 atccggctgc tgaacgcggc cacgggcgga acccgctatg aaatgctgaa cgtcggctgc
    17041 tcgtcgtatt cgacgcacca gagcctgatc gcgctggaac gctgggtgtg gccccgccac
    17101 ccggacctgg tcatcgtgta ccacgggtgg aacgaccaga ttgtccaggc ggatggattc
    17161 agcgaccacg aaaaagacgc cttgctgtcc ctggcccccg gcagccgccc ctcgtgggcc
    17221 gtgcgggtgc agaatatgcg gctggcccag ggcgtcggcc ggctgctgca agcggtcgat
    17281 cgctcctggc cgcgcccgcg ggtgcccgtg gaccggttcc ggagcaatct cgacgaattc
    17341 gcggaacgct gccggaagcg cgggacgcgc ctgatgatct ttgtccgccc ggaatcccgc
    17401 cgctacccgc tgccggcgta tgaagccggc gcgctggagc acttcagccg cgcctacaag
    17461 accaccaaca cgctggagct gtacaaggcc atccacgcgg attactccgc cgcgcagcgc
    17521 gagccgcggg agggcgccga agtgttcgat gcctgggccg tggtcaaccg gctgtacgag
    17581 cggcagcagg ccggcgagtt cgggcccggg gtggagatat ggcagaacga cagcattcat
    17641 ctccgcccgc tgggcgagga actgctggcc caggaactcg cgcccgccat tgcgccggag
    17701 gcggcggagg ccgtgaaaac ttttgtgcaa agcggtcgct attggtcctt cctggccggg
    17761 gagttcctga acgaacggct gccccacgag gccgtctatt gcgcacgccg ggccaaagaa
    17821 gccgatccgg cgctgaccgc ggcgatggac gagttgaccc gccgcgccga aaaggatttc
    17881 gagttcgtgg cgttgttcga ggagggccgg tggggcggca cggacccggc gttcgaatcg
    17941 aagctccgga aactctcgaa atgcctcggg atgcggcccg cggaccatgg cgtcctggtg
    18001 cagattttca ggacctgcct gtattcgaac cggccggaga tggcggcgga cgccctggcg
    18061 ggataccggc cggcctccgc cccgcaccga tacgaatggc tgaacatgcg gcttcagtcg
    18121 ctgctcgcgg ggaaccggct ccaggaagcg gaaggtgtgg ccgtcgagat gttgaacatg
    18181 aatccgcgcg aggcgcaggt gcgaggcgtg ctggaccaga ttcgcgcacg gaggacgcca
    18241 tgaacgggcc gttgacggac cggcaaaccg accgcatgga tcgcttcctg ttcgtcgcca
    18301 ttctcctcct cgtcgccgtt gccattttcc gctcgatcct tctgccgggg cggtttcttt
    18361 tcaccaccga cgacaacatc ggcgacctgg cgctgcggaa ggcgctgctc cccggcggct
    18421 attcgggcgg ctggtacgac ggcatgctgc tgggtttcaa catggtgatc cctcccggct
    18481 ggacccattt gctgctctgg ttgtcgccgc tgattccctt tgtcaactgg atgcacgggc
    18541 tggaccttgt gctggcctcg ctcgcgctgg cggcgttcct ccggcgcaag gacgtgagct
    18601 ggcccgcctg cgccgtcgcg gcgctcgccg ccttctgggt cggcagcaat ttcacgctga
    18661 cctacgccgg ccacatcatg aagttcggcg tgctgatgtg ggcggcggcc tacctctggt
    18721 gggtggatcg cgccgtggag accggccggc ccgccctcgc ggtcctggcg gggggggcgc
    18781 tgggcgcgat gttcctggag caggcggacg tggcgctgtt cttcgcgctc gcgctgggac
    18841 cgtatgccct tttccggctc gggcagacgg ccggggccca gggcaggaag tgggcggttg
    18901 tcctggttcc cctgctgctg tccgccttga taattgcctt ccacccgttg tgggaaggct
    18961 atcgcgccaa tgtgcagggc gtgacgtcga tggaacagga aaaccccggg gagaagtggg
    19021 cgttcatcac gcagtggagc tggccgcccg aggagagcat tgatttcgtc gcgcccggct
    19081 acatgggctg gcgcagcggg gagccggcgg gcccgtactg ggggcgcatg ggccggtccg
    19141 agggatggga gcaaacccgg cgcggcttcc agaatttcaa gctggagaac cagtacctgg
    19201 gcgccatccc gctcctcctg gccctggccg ccctggccgg cgcgcggcag aagcccgaga
    19261 tcattttctg gggcgtcgcc gcgctggtga cgctgctgct gtcgttcggg aagtactttc
    19321 ccctgtacgc cgtcttctac aagctgccgg tcattaacaa tatccgcaat ccgaacaagt
    19381 tcctgcaagt gttccagctg gtgctggcca tcctgtccgc ctatggattc gaccttgttc
    19441 tgaaggccgg ccggaagccg gatccgattg gagcgccgcc gcgcgcgtga gcgcgaacag
    19501 gccgctaccg gttcgcttcc aactgcgccc gcagggtgtc cacgtcgatc tcgtggacaa
    19561 agaagctctc gtcgccgcac aagacccgcc ggtccagcgg gaacgggccg atcacccgga
    19621 cgcccggggc ctgcaggggc atcagggtga tcagcaacac ctggtcgtag gtcccggaat
    19681 ccgctttccg gatcagcctt tggatggcct ccacgggcgg cactttcttg tcgttctcgt
    19741 accactgggc gtcgcagatc aggccctggt agaaacgctc ctgctcgatg caatagaacg
    19801 gcgggaactc gggccgcatg tagggttgaa tggactgggt ggcgtaggac agcacggtgc
    19861 ccacgagcac gttggtgctc ttgaggtggg cgttcaggaa ctgcgccgtc tcgcgtccgc
    19921 ccgaaaaggg gtgttgaaaa tccatgcgga atgccgtgac tgttccgatg gcttgcaggg
    19981 ccaggaacgc ggaaaagatc gcggcccgaa ggaggggata ccgcttcatc catctggagt
    20041 ccgcccaccg gccgggtgcc gggtaatagc ggccgatcca ctgggtgaag accagcagca
    20101 gccagagcat gccgatgaag cgcatgttgc tgccccggaa gaccagcagg taggcgatga
    20161 tcccgccggt gaccagaaag taacccaggg ggacaggctt ggcgtggacc aggtagagaa
    20221 ggccggccag gccaagcagg gccagcaggg tggagtgctg atcgcttacc gtgttggcgg
    20281 gccagaagta gaactgcggc ttcacgaccg gcgccgccag gcgaagcagg acgccccgcg
    20341 ccgtttggaa gtagtcgatc tctccgccgc cggacagcac cgcgcgcact tccgcaaacg
    20401 ggatcaactg gtagagcgac accaggaaaa caagcccgag cgcgaacagg ggcgccagga
    20461 agggggtgta ttcacgccat cgcgttcgat gggccgccgc caattcgatc acgtacagca
    20521 gcgccagcgt cccggcgaac aggccgccgt acatgtgggt ctgggccatc agggccagcg
    20581 ggagcatgta cagccagggg cgccggaagc gctgcgcgta gagcgtcgcc gccaggcaca
    20641 ggaaaagcag gatgggcacg tagttgcgcg ccacgatgtt gtactcgtag aagaccagga
    20701 acccgaaggg gtacaggatt ttttccagac gcgtgaacgg gccgaaacgg atgaacagga
    20761 aaacgcccgc gatcgccatc agcgaatgga aaacctgcat ggcgaggggc gaggggaaga
    20821 ccttgaggag gaaatacaag gcggtatgcc agccgccggg atccgtctgg tagccgaggt
    20881 catgcagcac ggcggccagc gaagggcccc atcgcgtata cagccagtcg gccgcctcat
    20941 cgcgccacag ttcgtggcgg gggatgagga cgaaggtcat cacgatgtac gccgccaggg
    21001 cgagataggg cagggcgtcg agcgcgcgac gggacgggct ggacacgggt tcgttcaccg
    21061 gtgtgttctc acgcgtcaga ctctaccagc gccatgatcg gcctgcaagc gggcgcccgg
    21121 cgatggacaa ggacccggcc ccgaggtagt atgcggcggg ccaggggccc gggctgatca
    21181 tatggcgggg aacaacaatt ccgtggatga aggccgcccg gccgcgtccg gccgcacggg
    21241 gctgatgctc ggcctggccg tcctgctggc gctgtcgatc ggcatccgtt tctacaagct
    21301 gggcgtgacg gacacgcgct cggacgaggt ggagctggtt gaacagctcc agggcggcgt
    21361 taaaccggtc gagtacttca agtggttcat cgaggtgttc caggccggcc gccagatgcc
    21421 tctgtcgcgc gtggcgacct gcatctatat gcggacgttc gaattgccgg tgaccctttt
    21481 ccacgtccgg accccgttcg ccatgttgag cgcgctgacg ccgctgttcc tggtgttcct
    21541 cgggcggtgg tgggccggcc cgcgcctggg ctggatcctc ggcgtcctgg ggctggtcaa
    21601 tccgttcgcc ctgttctggg ggcgcatcgg gctggcctat ggcttcgtgc tcccgttcgt
    21661 cgtcctgacc gcggccttct ttgtgccgtt gctgaacaac gccgccgggg ggcggccgtt
    21721 gccgcgcggc ctgctcgccg gtgtcacggc ggccgcggtc ctcggctcgt attcccacct
    21781 gtccgcgtgg cccttcacgg ggctgctctg gctggcgctc ttcttcctgg tgctgaaaaa
    21841 agcggcgtgg aatttccgcg cggtgcccgc gcgctgctgg atcgcgttcg cggtctgggc
    21901 gctctccatc gccccgtggg cctattcctt cctcacggcc gtcaacacca agtgggatat
    21961 cctcgacaag atttccaacg atcccgtgta cacgtttgcc gcgatgcgcc ggctgccctt
    22021 cgtgatggga tggggccggg gcgttccggg cgtgttcctg accctgggcc ttccgctgct
    22081 gggcctcgtc ctggcctgga agaaccgcgg cctgcgaccg ggcctggcgc tgacgctgct
    22141 gatcggcgtg gtcctctttg ccatgctggc gctcatgatg accttgagcg gcggcatcta
    22201 cacgcttcgc tactttacgc cgctgtggat cattccgatc ctgctggccg ggctggcgat
    22261 ccacgaggcg gcggcatggg tgggccggcg ctggccgcgt cccggggcgg cgaccctgtc
    22321 cgcggcgttg ttcctggcgg gcgtggcggc gcatgccgcc gcgccgctct ggtggaccgt
    22381 caacctgacg ggccatccca ccccctacag ccacgtcagc cggtggctgg acgcccacct
    22441 tccgaagggc gccccggcga tcgtggaccg atggttcgag cccagccacg agatgcggtt
    22501 ccacgcgccc acgaacacgt atgtgacctt caccgtcccg aacgaaccgc tgaagaattt
    22561 tctggaactc aagtggcgcg acacggtgaa gcagtttgcc ctgcgctacc cggacgcggc
    22621 ctatgtcgaa ctgatcaagt gctacttcta cgtgcccacg gtcggcggct gggaatggcc
    22681 gcgccagtat tttgcgcgcc acgcggtcat caccaacgag ccggcgctca agctccggca
    22741 gcgttggctg ggcatgaccg aggactacta cgccgacaac accaaccgca ttgtctgcga
    22801 aatcttttac aacaccaccg aggacatgct cgaaaaagcg cgcgcggccg gccagccgtt
    22861 cttcctccag tacggagagg gatggcgcta cgcgaaaacc caggatttcc gggattggcg
    22921 catgctggag ggcgcggcgg aagtcgaact cttcaatctg accggcgcgc cgcaggaggc
    22981 ccgcctgacg gtccggggcg ccgccgtgga gggaaccaag caggtgcgga gttccacggg
    23041 agcgcaggag accttcccgg ccaaccagag gcatgagtgg gccgtggtcc tgacgtcgct
    23101 tccgcccggc cggaccgtcg ttcggttgac cgacagcctg gggccggccg cccacgccgt
    23161 cctgctggtg gaagacctcc ggctggccgc ggatcaaggc gcttcgatca cgcggtagta
    23221 tcggtgcccg gtgtcccggg tgacgtccat cacgtacatg taggccccgg tgccggccac
    23281 cgggcttccg atgttctccc acaccgggtt gagcaggctc atggcgccct gcacctggta
    23341 ggtttccccg acgtatccga accacacgac gtccacggcg gaaacccgga tcgagatgtc
    23401 gcggtccacg ccgtactcga aggcccccat gtccaccagg ccgttcacga tgcgggggtt
    23461 gtgcgccaag tcggtggcgt ccggcatcca ggccatgttc tcgccgacgt ccacgcaggg
    23521 cgaaagattc tggaggcggt acgagccgtc cgcttcaaag agcgggttga tgttcgtatt
    23581 gcccgccagc cagttcaccg gtccgacgcc gtgcgtgaca atcccggcct ctccgccctg
    23641 cacgtccgag taggaaaggg tgatctcgat gtcgtaccat tccgggttga aggcaatctg
    23701 ctcggtcgtg ttgtcccaga gaatgcaatt atgcagagac ggactgctgt cgtaggaaac
    23761 catgactcct ccgccgtatc ccgacggcgc ggaattggcg gctatcgtca cgctgtccag
    23821 ctccgcgtcc gatccgtcaa agtaaaggcc gccgccctgg gtgatggacg aattccctgc
    23881 aatcaggagg ttacgggccg tgccggacgc gtggtagtag tggatggcgc cgcccccgcc
    23941 gtcggattcg ttgtgggtca gcacgaggtt ccgcagcgtg gggaggccgg cctgaaggcg
    24001 catgcctccg ccgccgtcgg cgctgttgcc gtcgatcacg cagtcgcata cgcagacatt
    24061 ggcgttgtaa aggctgatcc cgccgccctg tccgttcaac gcgaaattgc cgctgacaat
    24121 gcagttggaa accagcccgt ccgacgcccc gttaaagaaa aggccgcccc cccaggtatc
    24181 cttgtccgag cccgtcgccc tggcggtgcc attggtcagc gtaaagccga tgatcgccgc
    24241 attgcccgag gcataaacgc agcgaacagg cgtgcctcgg tcgcccgcga tcaccgtatt
    24301 ggtggggccg ttgaggcttt ggaggatgac gttgttggta atcaccacgc ggttcggcag
    24361 gatcgcgccg ggtgtgacgc ggctccccgt agaatacacc ccattgctta cgaggatcgt
    24421 gtcgccgtcc tgcgaagcgt ccagcgccgt ctggatcgtg gcggccgcca tggcccaatt
    24481 ggtgtacgga gggatggagg ccccggagag cgcgacgtag cgggtgatgg cccgggcgca
    24541 gggggcgatg gccagaccga tccaaagcgc atgaagagag aaaagccgaa cgcggcgctg
    24601 catgactcgc ctccttggtt gattagagga accagtcctc taaccccata ttcggcggcg
    24661 ttcgcctcat gtcaattcaa aagcgatttt ctcgaaatcc acttcggccg tcgccaggac
    24721 cttgtacggt ttcctgaacg cgcccgtgta atccaggaag gcccggaaat cgtgggttgg
    24781 aaccgcgaag tcgtcgaaga gcaggatggt gcccgtcttg agcagaccgt tcaacgtgca
    24841 cagccaatac aacgtcgagg tatagaggtc cgcatcgcag tgaacgatca ggcgcttctg
    24901 cggcttgtac tcggccagga atccggggag cgtgtcctga aacagcccct tgataaacgt
    24961 gacccgcgca tcgtccacgc ggggcacctg cccgtcgttg ctgaaggacc ccggctcgaa
    25021 ctttttcaac ccggtctgcc aggcctccgg caggcccgtg aaggtgtcga acccgtagaa
    25081 ccgggtttcc gggtgcgccg agagttggct ccactcgcgg atcgaatcgc cgaacatgac
    25141 cccggcctcg aggtagtcga acggctcgcc gcccatgaga cccgcgacat gctcgaagag
    25201 ttggaggcgg cgctcaaaat gccgcggcgc gggatgcgct cgcatccacc ggccgagggc
    25261 cagcagcgag ttgaagaagt caagggagga cgatggcgga atcccgcacc ggatgatgct
    25321 tctcttgatc tttcggcccc attccatttt taccctcctt gttgcgaaag catgatcact
    25381 gtccatcgtc gagcggcaga acatgaactt gttttttgtc ctcgccggag cgaggcgctt
    25441 cgtgccttca cgcctcttcg cccttcagcc acttcagcag ggcgggcatg tcgggatgga
    25501 agccctcctc gatccggacg tcccgttgct tttccgccgc cacgaagacc tgcgcgcccc
    25561 agcgcgggac gcgccgggcc acccagacgc ccagccggga gttctcaaag gggaagatat
    25621 agctcccttc gcgccggcgc accgcgaagc cgtgcttctc caggattcgc tggag
//
LOCUS       DAOOTK010000035        25479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1350_length_25479_cov_6.836021, whole genome shotgun sequence.
ACCESSION   DAOOTK010000035 DAOOTK010000000
VERSION     DAOOTK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25479
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1350_length_25479_cov_6.836021"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK634_05985"
     CDS             <1..334
                     /locus_tag="PK634_05985"
                     /inference="COORDINATES: protein motif:HMM:NF020136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HOW97320.1"
                     /translation="GGFSSLQYYFDPIDMSACRTAVIRLKGDGKRHQFLVEAERDARH
                     YLVYEFETGVDWQTVEIPLAERVPACRGDRLDIPNFPGQILAQVRFFIANRKAESLRL
                     EIDGIWLR"
     gene            584..1066
                     /gene="nuoE"
                     /locus_tag="PK634_05990"
     CDS             584..1066
                     /gene="nuoE"
                     /locus_tag="PK634_05990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOW97321.1"
                     /translation="MSQEIQADADLRAADIEAVIVPFRGRNGMLIPLLQKMQERFGYV
                     PPESIGPIAKALDVFPVEIYGILTFYAQFCLEPRGRHTIRVCQGTACYIMGGKDLLDH
                     VQGKLGIQVEQTTPDRRFTLERVACLGCCGMAPVVMVDNDFYGRCTPAKMDEVLELYK
                     "
     gene            1079..2845
                     /gene="nuoF"
                     /locus_tag="PK634_05995"
     CDS             1079..2845
                     /gene="nuoF"
                     /locus_tag="PK634_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOW97322.1"
                     /translation="MAALSSHRILICVGTSGVAAGAEKVAAAFQSELERRGLLEKFDI
                     VRTGDRGLFRDVLVDVITPELGRVVYEFIQPEHVAEIVEEHLVHGRPVEKRRAGQDYE
                     AFFKNQTRIVLANCGEIDPEHIEDYRRVGGYQALRKAMDMTPQAVIEEVHKAGLRGRG
                     GAGFSTGQKWRFCREAKGDLKYVICNADEGDPGAFMDRSLLEGDPHAVIEGMIIAGYA
                     IGAREGVIYCRAEYPLAVRRLTLAIEQARREGCLGSNILGCGFDFDVSIMQGAGAFVC
                     GEETALIASIEGRRGTPRVKPPFPAHQGLWGQPTLINNVETFGNIPPILRRGWEWFAA
                     IGTERSRGTKVFALAGQVRNAGLVEVPMGMTLRELIFGAGGGMSKRRIGLKGVQLGGP
                     SGGCVPESLLDTPIDYESITGTGAIMGSGGLIIMDERTCMVDVARYFLSFTVAESCGQ
                     CTPCRVGLKRMHEALEEITAGHGTPEHIAFLQEMGETIKSTALCGLGQTAANPVLTTL
                     RYFRHEYDAHVHDKECPAHACKALRHFEVIESACTKCGRCFKACPANAIEWKKGQAAR
                     IDLERCTRCQTCYGACDFMAIR"
     gene            2845..4944
                     /locus_tag="PK634_06000"
     CDS             2845..4944
                     /locus_tag="PK634_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW97323.1"
                     /translation="MKLTIDGKTVEARDGQTVLQAALDAGLYIPNLCYHPDLRPVGAC
                     RLCIVEIENMRGLPTSCTTPARDGMVVRTNTEAVLKLRQNLIWLILSEQSEPILEDSQ
                     LKKVVDWAGARNLLSSYRAQPKKRPVRDDDPYFIRDPNLCILCGRCVRICQDVRGVGA
                     IGIVNRGIESRVDTSAGADMRTAECRFCGACVEVCPTGALRDREPIPWADREKRILAC
                     QNACPAGIHIPQYVSLIAEGRFQEALEVIRDRAPFPFVLGCVCPHPCEQACRRCQMDE
                     PVAIRELKRFVASVDTGEWKARVTMRPPTGKKTAVIGSGPAGLTCAWFLRRLGHEVTV
                     YEAAPKPGGSLRASIPRYRLTDEVLDQEIRGIEEIGVKIVTGARVESLDPLFAEGVEA
                     VFLAVGAARGTKMGIPGEDDPRVQDGLTLLEAINRGHDVKLGGTVAVVGGGNVAMDVA
                     RTAVRLGAKRVVMLYRRTRQEMPASPEEIEYAIEEGVEIRYLVAPQRVVARPEKLQLE
                     CIRMELGEPDASGRRRPVPVPGSEFVQEADHLIMAIGQKPAVPESLGVALDRRDRLQV
                     DPATWSTSRPGVFSGGDVVSGPATVIEAINAGRNAAAAMDRYLGGSGRIEESLASLRK
                     QNPRLGREEHFAGKKRPKTPILPLERRTRSFDEVESSFNAATAVQEANRCLRCGLRLT
                     ICAAPLPPPTRPAEPAR"
     gene            complement(5070..6074)
                     /locus_tag="PK634_06005"
     CDS             complement(5070..6074)
                     /locus_tag="PK634_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOW97324.1"
                     /translation="MMQQSKSVKLAILKVLQELDTPAGAARIVQHLSSEGANLQPRTI
                     RFYLLQMDREGWTKTVSRRRGRELTAKGRDELAHANIIEKVGFISSRIDALSYRMTFR
                     NSAGVGTLIANVSTIEKSNLLRALEYMNPVFSRKLAMGNRLAVAREGASLAGFNVPKG
                     RVMLGTVCSVTVNGILLAERIPVTSRFGGLVEIRDGKPVRFVELIAYSGTTIDPLEFF
                     INAGMTRVRECAKTGSGIVGASFREVPAVALDDVRRIGKEMERQGLGGLLALGQPNQP
                     LLDIPVAEGRCGMIVLGGMNPIAALREAGVLVTIQSLAGLDECSTFQPFQSLRNRYPG
                     "
     gene            6327..7664
                     /gene="gdhA"
                     /locus_tag="PK634_06010"
     CDS             6327..7664
                     /gene="gdhA"
                     /locus_tag="PK634_06010"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOW97325.1"
                     /translation="MSYIQDVLQMVEKRNVGEPEFMQAVREVLESLDPVIQRHKKYEE
                     GRILERLVEPERQILFRVPWQDDQGRIQVNRGFRFEFNSAIGPYKGGLRFHPSVNQSI
                     LKFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSHHEVMRFCQSFMTELFRHIGSDTD
                     VPAGDIGVGGREIGFLFGQYKRLKNEFTGVLTGKGLKWGGSLIRPEATGYGSVYFAEE
                     MLKTRGETFKGKVCAVSGSGNVAQYTVEKLNQLGAKVVTLSDSNGTIYDKAGITAEKL
                     AFAMELKNVKRGRIKEYADHFKCEYQEGKTPWFVPCQCAFPSATQNEITGEDAQMLLK
                     NGCYLVAEGANMPSTPAAIDQFLQAKILYGPGKAANAGGVATSGLEMAQNSMHLTWSR
                     EEVEQRLKGIMVSIHKACFETAKEYGQPGNYVMGANIAGFVKVADAMLDQGLV"
     gene            8049..9335
                     /locus_tag="PK634_06015"
     CDS             8049..9335
                     /locus_tag="PK634_06015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOW97326.1"
                     /translation="MKDKAYNLFEMAQRQFDQVAGLLELDDGVRQLLRQPLREFQFLI
                     PVRMDDGQVKVFNGFRVQHNDARGPAKGGIRFHPHENIDTVRALAMWMTWKCSVVDIP
                     LGGGKGGVICDPHNLSAREQEQICRGWVRQMAKNVGPFQDVPAPDVMSTGQHMLWILD
                     EFETIHGGRYPGFITGKPVGMGGSLGRTEATGFGVIFVLREAMKVLGIDPSKTTASVQ
                     GFGNVAQYAIRKYHELGGKVTCVACWNNKDKTSYAFRKKDGIDADQLAGIADSFGDMD
                     KDKARGLGYEVLPGDEWIAQDVDILIPAALENQVTAANAGKIGPRVKIIVEGANGPTT
                     PEADEMIKARKIFLIPDFLANAGGVTCSYFEQVQCNMNYFWEKKEVLEKLDSKMTAAF
                     HAVNQLAQKRKLYMRDAAYVIAIDRVAQAVKSRGWV"
     gene            9346..12534
                     /locus_tag="PK634_06020"
     CDS             9346..12534
                     /locus_tag="PK634_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOW97327.1"
                     /translation="MPSRRNSRGQSLDNMLEALRERAKELDCLYHVEELLDQHDLPLA
                     DLFQRMAGIIPSGWQFPDICQARITFEDAVYKSPDYAPAVCGHAADIRVEGMKSGSLE
                     VSYRQEVPMKGNDCFLEKEHKLIHTIADRIGQTVQHRRLRQMRTEWETARAELTGQNS
                     REWMVIVDLIRRTDPAMLNYLSRQMLYRLFWHGITEAREIVRHSDLIGDEHLHEESNS
                     PTETQTVEKMLTLSEAVFALAEKHLSPEDILASIQKWIQEKRTSFLIKAVDNIDTTLE
                     EIIDAVMLYKTMTEKAVSLPPTTEKWLRVSLLRRFFSDHLEYLKTAKENVEVADFHDL
                     VQHIIYPAGSSGNLGGKSAGLFLAYSILSKDREAAMENLKVPKTYYIPADALTVFLHI
                     NDLEAINEQKYKDLEQVRLEYSNIIHLLKCSRFPTDVVKGLTAALDDFGDTPLIVRSS
                     SLLEDRVGASFSGKYKSLFLANHGTRPERLQALLDAIAEVYASMFGPDPIEYRGAWGL
                     LDFHEKMAIMIQQVVGRRIGSYFLPAFSGVAFSNNEFRWSPRIKREDGLVRLVPGLGT
                     RAVDRVSDDYPILISPGAPELRVNVTPDEVRRYSPKRMDVINLEAGRFETVNTKDFLR
                     KHGPEIPGVNQMVSVFHEGGIPAPAGLSTDFANDDVIVTFEGLVRKTTFMKQLQAMLK
                     RLQEKMGRPVDIEFASDGTHFYLLQCRSQSLTGSNLPSAIPSDIPEDRIVFSANRFVS
                     NGTIPEITHIVYVDPDGYAALTSRNDLLDVGRAVGMLNRLLPKRQFILMGPGRWGSRG
                     DIKLGVQVTYSDINNTAVLIEIARRKGNYVPELSFGTHFFQDLVEASIRYLPLYPDDE
                     GVRFNEAFLKQSPNILPGILRPFEHLAGAVRVIDVPKSTGGRILSILLNADQVEALGF
                     LAEPLDRTEDAPRAPEAAHEPPPAKDHWRWRLRMAEQIAERLGPAHFGVKAMYVFGST
                     ANASAQPGSDIDLLIHFAGTEQQRADLMTWLQGWSSSLAEMNYLRTGHQSTGLLDVRL
                     VTDEDVAGKKGYAAKIGSVTDPARPLRLKQDG"
     gene            complement(12658..15267)
                     /locus_tag="PK634_06025"
     CDS             complement(12658..15267)
                     /locus_tag="PK634_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOW97328.1"
                     /translation="MLDPTLTTGLPALDQVLKGLLPGDNIVWQVDAVEEYQEWVEPFG
                     RAAIRNGKRLIYFRFARHQSLLSAGKGIEVHELDPEQGFETFIATIHSVIDQAGPGVF
                     YVFDCLSRLAADWCSDQMLANFFMLTCPYLYDLETVTYFALYRNYHTSHAIGPIQATT
                     QLFLDTYRHKGDLYVRPVKVQHRYSPTMHMLHVWHGDSFRAVTASAVISEILTLSQWS
                     GLASDSSIGFWETSFIRARELIQSGEYRPDLPGRGKDVFETLIKMIISRDPGMQRLIS
                     RYLTLQDILDVRKRMIGTGLIGGKTVGMLLSRAIVKKADRRFVEEMEEHDSFFVGSDV
                     FYDFLVRCGVWWIRQKQRNPETFLEGAEQARRRIITGEFPPYILRQFEEMLDYFGQSP
                     FIVRSSSLLEDNYGHSFAGKYESVYCANQGPRERRMQDLLAAIRTIYASSMSEKALSY
                     RADRGMLDRDEQMALLVMRVSGAMYGRNFYPQMAGVGFSFNPYAWAPEIDPKAGVIRL
                     VFGLGTRAVNRSDDDYTRIVALNAPEKRPETNFDEVAQYAQRRMDYLDLEANQLVSDY
                     FTDVVTGDEDFPIEMFASEDAAPSARGGKPHRILTFDSLLARTGFVKDLREILHVLQV
                     AYNHPVDIEFTANFVGDGRYKINLLQCRPLQVQGAALVEMPDVKVGREDLIIAARGAV
                     IGQSRLNRIDRLVYVSPDLYSRLPVQDRHEVARLLGEINRARGAAGGPCRAGVETVML
                     LGPGRWGSGSPHLGIPVTFSEINRASILCEIVAMHENLVPDVSLGTHFLNELVERNIL
                     YLALFPQQGENFLNKEFFEKTPSRLLEIVPGAGKMKEVVRVLDAAQIGGGAIIKIMAD
                     AVRQRVLVYFERD"
     gene            15476..16726
                     /locus_tag="PK634_06030"
     CDS             15476..16726
                     /locus_tag="PK634_06030"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOW97329.1"
                     /translation="MNEKVNTFEVAQAQFDEAADRLGLESSLREFLRWPQREYKFTVP
                     VKMDDGSTKTFHGYRIQYNDARGPCSGGLRWHANESLDAGRASAAWMTWRTALVDIPL
                     GGASGGLVCDLRKLTEGERERLARGWMRIMAQPLAEHRDVLTPDLYTNPQIMAWMADE
                     FGSVMGHARPGVVSGKPLALGGILGWSDATARGGVLVVGEACQTLGIDPKGTYAIQGF
                     GNAGQNVALLHPEILGGGRLVAVSDTSGGVINTEGIDPKALVAYKLKTGSVTGFPGAQ
                     PVCGEDLLDLDVDVLYPAALENVITRMNAEQIKAKIVCELADGPTAPEADRTLHIKGV
                     HIIPDILASAGSVTVGYFEQVQNASNYTWPLDSVHKQLARIMAHAYRNVLTMHKKQNV
                     PMRLAAYLVAVHRVAEAVKLRGWV"
     gene            complement(16782..17345)
                     /locus_tag="PK634_06035"
     CDS             complement(16782..17345)
                     /locus_tag="PK634_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOW97330.1"
                     /translation="MADIEFVSDAEIYRRVIQAEVPQARRFLWLATADLKDLHVAHGR
                     HMIPFLGVLSSLCEQGVAIRLLHAKEPGPNFRRDFDRYANLVTDMERILCPRVHFKSV
                     IVDGRLAYTGSANLTGAGMGAKSPHRRNFEAGIITTEASLVQQIMDQYDRVWMGAYCD
                     DCGRQRHCPDQIADVGPRRHHRADRSR"
     gene            17632..18765
                     /locus_tag="PK634_06040"
     CDS             17632..18765
                     /locus_tag="PK634_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOW97331.1"
                     /translation="MRKKESKTTHYHGFAIRPTGFGTWMADNCDRTKRLRKCFDTLAE
                     AKTWAENKSVDLKNHGLLALTLSDPDRRDAVAALTILSGATTLEKAAREYIRRHPQHN
                     AEKLSWTCWRYLRQLYREGARPVTIKNARWKLRLWCKTEGENLTAGISRTEVDAWIER
                     RGYKGQSRRGYERQLNALLSFYAGTRKRRELADATPPPVFTPQDVERLMFAAEQHDPG
                     MVAPLTLLLFCGLRPYEMMRLTWDKIHLAEGEVIVPPEASKVRQARAVKLEPCALRWL
                     AASRGSGNVAPGQYELRRRREALMEKAEIEAWPPDVARHTFASALYALKSDAAYVAAQ
                     LGHFGSLGVFARHYKAVMRQADAERFWTIEPHKKENVMVLGAS"
     gene            18804..19589
                     /locus_tag="PK634_06045"
     CDS             18804..19589
                     /locus_tag="PK634_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97332.1"
                     /translation="MKERDYERILASLSPEQHKRLLEIAELDAKVPKQEKKVRRFFKQ
                     RAKKREEEIKKLVSLSPKDVLEQCRCWILDFFDDRIIEKQFGVTGIKSAFAPQQKNTH
                     SQSLEDLRQSFCSWLSRLSDRTQWRYFTTLLKYRAIPTEFFIEPFFQYIKELADHPEK
                     AARSAGKAAIVYSMTGRGGSIKGGRLNRLQSSEILGVCLKTFDGMRKQTKCPDSWLGR
                     KETNERVVTYDAEQVYDLTIERLHTSLSVEAEKKREAERNRRE"
     gene            20515..20760
                     /locus_tag="PK634_06050"
     CDS             20515..20760
                     /locus_tag="PK634_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97333.1"
                     /translation="MSVEIFKGSESERYTPRTKAFCRRCNQNTPTEIHELPGGLGNCC
                     AWCGCCRKGKPYFSKAEADSLKRLFAAEGEHEQATPC"
     gene            20738..21964
                     /locus_tag="PK634_06055"
     CDS             20738..21964
                     /locus_tag="PK634_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97334.1"
                     /translation="MSKRHLVEPRAELESYIRKHFPDLTGPEMNVYFNALPHLETIKP
                     KSAYGAKVLASEDDWCMASGHNRRTVHKALVDLAARGPISLTPGDNGRTTGKRKAAIV
                     RRNSIAEIQTACPREVLTAFVPADAEQLCAILNERGIPWHGQTIKPRWTVQRTGRIGY
                     GGSIMVQPGHNTKAARIEAFRASLRPGERLIEADFSSAEPSLLCHELEQNNLIPPGID
                     PAKIYSEIQRAAKSSRDDAKTRFQKTVYSPYPNLYVPEAWNLEQGHFLREVISGAERY
                     RNRLWDQGKPTKDQPRHVHTATGRLIQGVSRERIHRGKVLAWRLQGTIADIFLSIIPA
                     VLEAEQRGECRLFLPLHDALYVSVPEGSTFNPARLMQEKAAELGLPLKVTTKEHGQGG
                     GQRRRPGGRRDRTGLE"
     gene            22548..23777
                     /locus_tag="PK634_06060"
     CDS             22548..23777
                     /locus_tag="PK634_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOW97335.1"
                     /translation="MNLRRTGLLLTVVMQAHLSMAEMFDIEAFRSNGLLMWTDIYSNG
                     VYSIQWASSPTDEWNKAWSKLESLTATGGTMSVNIPMYFRITYRPFFAPHTNIVLVAG
                     GGQPLGPPYDFYMSKYEVQNCEWVDFLNDAQIHTNDIRGTNMFFASGGNVYMNSSMSS
                     DEILFSIASSDLIYDTGNPVGARYSVWLVPSNRPITGVSWYGSLKYCNWLTLVAGRGE
                     DQRCYSEGTSGSAWHPSGIAYADWYDNFSPPERLAWLNNFRGFRLPMDDYCLRASYFN
                     EWYKAAAWNGQTNTTYAYGRNSIDGQDACWMSSSHPYRMYTIGTTPVGYYDGTSHGGS
                     FQTRASSNFFGLYDLSGNVAEWLSDAWGPNDPDMRTRGGFWSANFDYAATDYLDWDRQ
                     KSTCANYLGFRIVTTGP"
     gene            complement(24046..>24465)
                     /locus_tag="PK634_06065"
     CDS             complement(24046..>24465)
                     /locus_tag="PK634_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97336.1"
                     /translation="PVHFYWVKGHSKDEHNKAVDKAAKASAKAPLNAPLTRVNVRRKH
                     TTRPLSRGSVEMMGQRITIRIVTSEWLPIQRLWKYRYEVVSKASRFRGNIDFIYSRLH
                     LGAGHTYYVLFNSDAANPQVEKLFRETKPKQRITGQD"
     assembly_gap    24466..24475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24476..24861)
                     /locus_tag="PK634_06070"
     CDS             complement(<24476..24861)
                     /locus_tag="PK634_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97337.1"
                     /translation="MITKENALNIYIDGSSRNSPRVGGIGVRFILTNSVGQDETEDIP
                     FLGYRGATNNQMELQAGIMALKEMKKRTLPDYIRQVAIHSDSRYVTDNYQRALFQWSG
                     NRWRNQNGRPVDNAEQWKELVKLIRK"
BASE COUNT         5139 a         7787 c         7864 g         4679 t
ORIGIN      
        1 cggcgggttt tcgtccctgc agtactactt cgacccgatc gacatgtcgg cctgccgcac
       61 cgccgtcatc cggctaaagg gcgacggcaa gcgccaccag ttcctcgtgg aggcggaacg
      121 cgacgcgcgg cattacctcg tgtacgagtt cgagaccggc gtggattggc agacggtgga
      181 gatccccctc gcggagaggg tccccgcgtg ccgaggagac cgcctggata tcccgaactt
      241 ccccggtcag atcctggcgc aggtccgatt cttcatcgcc aacaggaaag cggaatccct
      301 ccgccttgaa atcgacggga tctggctccg gtaggctcgc tcctgcagga tatctcggtc
      361 gccttcacgg cacggcgcgc cgggatcaga aggcctcttg ccggtcgaaa gaatcgcact
      421 ctcatcgcgc ctattcagcg gaccgaagcg gtcggtcagc gcccgatcgg ggccgtacga
      481 aaaaacttga gaaaacggcg cctccggata gagtgcccgg ctcctgtgtt gcacaggggc
      541 cgaagggcgt gtaagttttc caacggcgca tcagacctcg aaaatgagtc aggaaataca
      601 agccgatgcg gacctgcgcg ccgctgacat cgaagcggtc atcgtcccgt tccgcgggcg
      661 caacggcatg ctgattccgc tgttgcagaa gatgcaggag cggttcggct acgtgccgcc
      721 ggagtccatt gggcccattg cgaaggcctt ggacgtgttc cccgtcgaga tttacggcat
      781 cctgacgttt tacgcgcagt tctgcctcga gccgcgcggc cggcacacca tccgtgtctg
      841 ccagggcacg gcctgctaca tcatgggcgg caaggacctg ctcgaccacg tgcaaggcaa
      901 actgggcatc caggtcgagc agaccacgcc ggaccgccgc ttcacgctcg agcgcgtagc
      961 ctgcctgggc tgctgcggca tggcgccggt ggtgatggtg gacaacgact tctatgggcg
     1021 ctgcaccccg gcaaaaatgg acgaggtcct ggagttgtac aagtaaggtc ttgccctcat
     1081 ggcggctcta tcttcccatc gaatcctcat ctgcgtcggc accagcggcg tggccgcggg
     1141 cgccgagaag gtggccgccg ccttccagtc ggaactggag cggcgcggct tgctggagaa
     1201 gttcgacatc gtccgcaccg gcgaccgcgg cctgttccgg gacgtgctgg tggacgtgat
     1261 cacgccggag ctggggcggg tggtgtacga gttcatccag cccgagcacg tcgccgagat
     1321 cgtcgaggaa cacctcgtcc acggccggcc ggtcgagaaa cggcgggcgg gccaggacta
     1381 cgaggcgttt ttcaaaaacc agacgcggat cgtcctggcc aactgcggcg agattgatcc
     1441 ggagcacatc gaggactacc ggcgggtcgg gggctaccag gccttgcgaa aggccatgga
     1501 catgacgccg caggccgtga tcgaagaggt tcacaaggcc ggcttgcgcg gccgcggggg
     1561 ggcgggcttc tcgacagggc agaagtggcg attctgccgc gaggccaagg gggatcttaa
     1621 gtacgtcatc tgcaacgccg acgagggcga cccgggcgcg ttcatggacc gcagcctgct
     1681 ggagggcgat ccgcacgccg tgatcgaggg aatgatcatc gccggctacg ccatcggggc
     1741 gcgggagggc gtgatctact gccgggcgga gtacccgctg gccgtccggc ggctgaccct
     1801 ggccatcgag caggcgcggc gggagggctg cctgggctcg aacatcctgg gctgcgggtt
     1861 cgacttcgat gtgagcatca tgcagggcgc gggggcgttc gtctgcggcg aggagacggc
     1921 cctgatcgcc tccatcgagg ggcggcgcgg cacgccgcgc gtcaaaccgc cgttccccgc
     1981 gcaccagggg ctgtggggcc agccgacgct gatcaacaac gtcgagacct tcggcaacat
     2041 cccgcccatc ctccggcgcg gatgggaatg gttcgccgcg atcggcacgg agcgcagcag
     2101 ggggaccaag gtcttcgcgc tggcgggcca ggtgcggaac gccggcctgg tcgaggtgcc
     2161 gatgggcatg acgctgcgcg aactcatttt cggggcgggc ggcggaatga gcaagcggcg
     2221 gatcggcctg aagggcgtgc agctcggcgg cccctcgggc ggctgcgtgc cggagtcgct
     2281 gctggacacg cccattgact acgagtccat caccggcacg ggcgcgatca tggggtccgg
     2341 cggcctgatc atcatggacg agcgcacctg catggtggac gtggcgcgct acttcctgtc
     2401 cttcacggtg gcggagtcgt gcggccagtg caccccctgc cgcgtcgggc tcaagcgcat
     2461 gcacgaggcg ctggaggaga tcaccgccgg gcacggcaca ccggagcata tcgctttcct
     2521 ccaggagatg ggcgagacga tcaagagcac ggccctgtgc ggcctcggcc agaccgcggc
     2581 caaccccgta ctgaccacgc tgcgctattt ccgccacgag tacgacgcgc acgtgcacga
     2641 caaggaatgc cccgcgcacg cctgcaaggc cctgcgccat ttcgaggtga tcgagtcggc
     2701 ctgcaccaag tgcggccgct gtttcaaggc ctgcccggcg aacgccatcg agtggaagaa
     2761 gggccaggcg gcccggattg acctcgagcg gtgcacccgc tgccagacgt gctacggcgc
     2821 ctgcgatttc atggccatcc gatgatgaaa ctgaccatag acggcaaaac ggtggaagcc
     2881 cgggacggac agacggtcct gcaggccgcc ctcgacgcgg gcctctacat tcccaacctc
     2941 tgctatcacc cggacctgcg gcccgtcggc gcctgccggc tgtgcatcgt ggagatcgag
     3001 aacatgcgcg gcctgccgac gtcctgcacg acgcccgcgc gcgacggcat ggtcgtccgc
     3061 accaacaccg aggcggtcct caagctgcgc cagaacctga tctggctgat cttgagcgaa
     3121 cagtcggagc cgatcctcga ggacagccag ttgaagaagg tcgtggactg ggcgggcgcg
     3181 cgcaacctgc tttcgtccta tcgtgcgcag ccgaaaaagc ggccggtccg cgacgacgat
     3241 ccctacttca tccgcgaccc gaacctgtgc atcctgtgcg ggcgttgcgt gcgcatctgc
     3301 caggacgtcc gcggcgtcgg cgcgatcggc atcgtcaacc gcggcatcga gagccgggtg
     3361 gacaccagcg ccggcgcgga catgcggacg gccgaatgcc ggttctgcgg cgcgtgcgtc
     3421 gaggtctgcc cgaccggcgc gctgcgcgac cgggagccca ttccctgggc cgaccgcgag
     3481 aagcggatcc tggcctgcca gaacgcctgc cccgcgggca tccacatccc gcagtacgtc
     3541 tccctgatcg ccgagggccg gttccaggaa gcgctggagg tcatccgcga tcgcgcgccg
     3601 ttcccgttcg tgctcggctg cgtctgcccg catccgtgcg agcaggcgtg ccgccgctgc
     3661 cagatggacg agcccgtggc catccgcgag ctcaagcgct tcgtcgcctc cgtggacacc
     3721 ggcgagtgga aagcccgcgt caccatgcgg ccgccgacgg gaaagaagac cgccgtgatc
     3781 gggtccggcc cggccggcct gacctgcgcg tggttcctgc ggcggctggg ccacgaggtc
     3841 acggtctacg aggccgcgcc gaagcccggc ggcagcctgc gcgccagcat tccgcgttac
     3901 cgcctgacgg acgaggtgct cgaccaggaa attcgcggca tcgaggagat cggcgtgaag
     3961 atcgtcaccg gcgcgcgcgt ggaatccttg gacccgctct ttgcggaggg tgtcgaggcg
     4021 gtcttcctgg cggtcggcgc cgcgcgcggg accaagatgg gcatcccggg cgaggacgat
     4081 ccgcgcgtgc aggacggcct gacgctcctg gaggcgatca accggggcca cgacgtgaag
     4141 ctcggcggca cggtggccgt ggtcggcggc ggcaatgtcg ccatggacgt cgcgcgcacg
     4201 gcggtgcgcc tgggcgcgaa gcgcgtggtc atgctctacc gccggacccg ccaggagatg
     4261 cccgcctcgc cggaggaaat cgagtacgcc atcgaagagg gcgtggagat ccgctatcta
     4321 gtcgccccgc agcgggtcgt cgcccgcccg gagaagctgc aattggagtg catccggatg
     4381 gaactgggcg agcccgacgc cagcggccgg cgccgccccg tcccggtgcc gggcagcgag
     4441 ttcgtccagg aggcggacca cctgatcatg gccatcgggc agaagcccgc cgtgccggag
     4501 agcttgggcg tggccctgga ccgccgggac cggctccagg tggatcccgc gacgtggagc
     4561 acctcgcgcc cgggcgtgtt ttccggcggc gacgtggtca gcggccccgc gacggtcatc
     4621 gaggccatca acgccggccg aaacgccgcc gcggcgatgg accgatacct cggcggttcg
     4681 ggccgcatcg aggaatccct ggcctccctg cgcaagcaga acccgcggct cggccgcgag
     4741 gagcatttcg ccggcaagaa gcgtccgaag acgcccatcc tgccgctgga gcggaggacc
     4801 cggtcgttcg acgaggtgga gtcctcgttc aacgcggcca cggccgttca ggaagcgaac
     4861 cgctgcttgc gctgtggcct gcggttgacc atctgcgcgg cgccgctgcc gccgccaacg
     4921 cgcccggccg aacccgcccg gtaaaaaaac gccgcgcccc ggatcgttca ggctggaaac
     4981 tggagagcag gaactgatcc ccgccgatcg gcagcacgcg acttgtggtc atgatatcga
     5041 agtcgccgtg gccggatgga tggtgtccct catcctggat agcggttccg tagcgactgg
     5101 aaaggctgaa atgtgctgca ctcatcgaga cccgccagcg actgaatggt caccaggaca
     5161 ccggcttcgc ggagggcggc gatcggattc atcccgccga gcacgatcat gccgcaacgg
     5221 ccctccgcca ccggaatatc cagcaacggc tgattcggct ggcccagggc aagaagcccc
     5281 cccaatccct gccgctccat ctccttgcct atgcgacgca catcgtccag cgccacggcc
     5341 ggcacctcgc gaaaactggc ccctacaatg ccggaacccg tcttggcgca ctcgcgcacg
     5401 cgggtcatgc cggcgttgat gaagaactcc agcggatcga tggtcgtgcc gctgtaggcg
     5461 atcagctcga cgaaccggac cggcttgccg tcgcggatct ccaccaggcc gccaaaccgg
     5521 gacgtcaccg ggatgcgctc ggccagtaga atgccgttga ccgtcacgct gcacaccgtg
     5581 ccgagcatca cccttccttt cggcacgttg aagccggcca gcgacgcccc ttccctggcc
     5641 acggccagcc gattccccat ggcgagtttc cgcgaaaata cggggttcat gtattcgagc
     5701 gcacgcagca gattgctttt ctcgatcgtc gaaacattgg caataagcgt cccgacgccc
     5761 gcactgtttc ggaacgtcat gcgatagctc aacgcatcaa tccgcgaaga gataaatccg
     5821 actttctcga taatgttcgc gtgcgcgagt tcgtcgcggc ccttcgcggt cagttcgcgg
     5881 cccctccggc ggctcacggt tttcgtccag ccctcgcggt ccatctggag caaataaaag
     5941 cggatggtgc gcggctggag attcgcgccc tcggatgaaa gatgctggac gatgcgagcc
     6001 gccccggccg gagtgtccag ctcctgaaga actttcagaa tggcgagttt gaccgatttg
     6061 ctctgctgca tcatcgagtc ctccttacga tttcctgaca cccgccctgc cgtttccgtt
     6121 tgccgcttgt gctcacccta tcgatgctaa ttgctcaaaa aacgggcatc tcgcgactgc
     6181 tcattgttat aatgatacgg caatattgcc atatcagcaa taatactttt aatatgaata
     6241 tatttggaag agtgctcgcg ggtgtctact ttatggctgt ccattaccgt attgagtcac
     6301 tgaacgaatg aataaggagc aagttcatga gttacattca ggacgtactt cagatggtgg
     6361 aaaagcgaaa tgtcggggag ccggagttta tgcaggcggt gcgcgaggtg ctggagtctc
     6421 tcgatccggt cattcagcgc cataagaaat acgaggaagg gcgcattctc gaacgtctcg
     6481 tggagcccga gcggcagatc cttttccgcg ttccgtggca ggacgaccag gggcgcatcc
     6541 aggtcaaccg cgggtttcgc ttcgagttca acagcgcgat cggcccctac aagggcggct
     6601 tgcgctttca tcccagcgtc aaccagagca tcctgaagtt cctcggcttc gagcagatct
     6661 tcaagaacgc gctgacgacg ctccccatgg gcggcggcaa gggcggttcg gacttcgacc
     6721 cgaagggcaa gtcgcaccat gaagtcatgc ggttctgcca gagcttcatg accgagctgt
     6781 tccgtcacat cggctcggac acggacgttc cggccggaga tatcggcgtg ggcggccgcg
     6841 agatcggttt ccttttcggc cagtacaagc gcttgaaaaa cgagttcacc ggcgtcctca
     6901 ccggcaaggg cctgaagtgg ggcggctcgc tgatccggcc cgaggcgacc ggctacggtt
     6961 cggtctactt cgccgaggaa atgctcaaga cccgcggcga gacgttcaag ggcaaggtct
     7021 gcgcggtgtc cggctccggc aacgtggccc agtacacggt cgagaagttg aaccagctcg
     7081 gggcgaaggt ggtcacgctt tccgactcga acggcacgat ctacgacaag gccggcatca
     7141 ccgccgagaa gctcgcgttc gccatggagc ttaaaaacgt caagcgcggt cgcattaagg
     7201 agtacgccga ccatttcaag tgcgagtacc aggagggcaa gacgccgtgg ttcgttccct
     7261 gccagtgcgc ctttccgagc gccacacaga atgagatcac gggcgaggac gcccagatgc
     7321 tgctgaagaa cggctgctac ctcgtcgccg aaggcgcgaa catgccgagc accccggcgg
     7381 cgattgacca gttcctgcag gcgaagatcc tctacggacc gggcaaagcg gccaatgccg
     7441 gcggcgtggc cacctccggc ctggagatgg cccagaattc gatgcacttg acctggtcgc
     7501 gcgaggaagt ggagcaacgg ctgaaaggga tcatggtctc gatccacaag gcgtgcttcg
     7561 agacggccaa ggaatacggc cagcccggca attatgtcat gggcgccaac atcgccggct
     7621 tcgtcaaggt cgcggacgcg atgctggacc aggggctcgt gtaatcggtg acacaacgtc
     7681 cttggggatc cggcgggcgg ctgaaatagc cgcccgcctt ttttttcgcg cggcacggcc
     7741 cgcgcccaat attccgctag cccggtttat tcatgaacgc cagccgttca tgaataaacc
     7801 gcttggcaca cgggctgcgg tgcagtccgt gcgccaagac gtgacgggac acggtgtgcg
     7861 ccgtgtcccg aacgtggcaa gccccgtttc ggcccgccgt ggcgggccga tcacggccgc
     7921 gaagcggcgg attattcgcg cttcgcgaat aatccgggct aggcaggtcc gcggcgtctg
     7981 tgataggagg gaagtggctt ttcccaggga gtgcatcctc acatcaagaa aggaagaacc
     8041 gcgcgacgat gaaagataaa gcgtacaatc tgttcgagat ggctcaacgt cagtttgacc
     8101 aggtagcggg cttgctcgaa ctcgatgacg gggtccgaca gcttctgcgg cagcccctgc
     8161 gcgaattcca gttcctgatc ccggtgcgca tggacgacgg ccaggtcaag gtcttcaacg
     8221 ggttccgcgt gcagcacaac gacgcgcgcg gcccggccaa ggggggcatc cggttccacc
     8281 cgcacgagaa catcgacacg gtccgcgccc tggccatgtg gatgacctgg aagtgctccg
     8341 tggtggacat tcccctcggc ggcggcaagg gcggcgtgat ctgcgatccg cacaacctga
     8401 gcgcgcgcga gcaggagcag atctgccggg gctgggtccg ccagatggcg aagaacgtgg
     8461 gccccttcca ggacgtgccc gcccccgacg tcatgagcac cggccagcac atgctgtgga
     8521 tcctggacga gttcgagaca atccacggcg ggcggtaccc gggcttcatc accggcaagc
     8581 ccgtcgggat gggcgggtcg ctcggccgga ccgaggccac gggcttcggc gtgatcttcg
     8641 ttctgcgcga ggcgatgaag gtcctcggga tcgacccgtc gaagaccacc gccagcgtcc
     8701 agggcttcgg gaacgtggcg cagtacgcca tccggaagta ccacgaactg ggcggaaagg
     8761 tgacctgcgt ggcgtgctgg aacaacaagg acaagacgtc ctacgcgttc cggaaaaagg
     8821 acggcatcga cgccgaccaa ctggccggca tcgccgactc cttcggcgac atggacaagg
     8881 acaaggcccg cgggctgggc tacgaggtgc tgccgggcga tgaatggatc gcccaggacg
     8941 tggacatcct gatcccggcg gccctggaga accaggtcac cgcggccaac gccggcaaga
     9001 tcggcccgcg cgtgaagatc atcgtcgaag gcgccaacgg ccccaccacc cccgaggcgg
     9061 acgagatgat caaggcgcgg aagatcttcc tgatcccgga cttcctggcc aacgcgggcg
     9121 gcgtgacctg cagctacttc gaacaggtcc agtgcaacat gaactacttc tgggagaaga
     9181 aagaggtcct ggagaagctg gactcgaaga tgacggcggc ttttcacgcg gttaaccaac
     9241 tcgcccagaa gcggaagctc tacatgcggg acgcggccta cgtgatcgcc atcgaccggg
     9301 tggcccaggc ggtgaagagc cgcggctggg tctagaagcc tgaccatgcc atcccgacgg
     9361 aacagccggg gccagtcgct ggacaatatg ctcgaggccc tgcgcgagag ggccaaggag
     9421 cttgactgcc tctaccacgt ggaggaactg ctcgaccagc acgacctccc gctggccgac
     9481 ctgttccagc gcatggccgg catcattccc tcgggctggc agttcccgga catctgccag
     9541 gcgcgcatca cgttcgagga cgcggtgtac aagtccccgg actacgcccc ggcggtctgc
     9601 ggtcacgccg cggacatccg ggtggagggg atgaagtccg gttcgctgga ggtctcctac
     9661 cggcaggaag tgccgatgaa gggcaacgac tgcttcctgg agaaggaaca caagctcatc
     9721 cacaccatcg ccgaccgcat cggccagacc gtccagcacc gcaggttgcg acagatgcgc
     9781 accgagtggg agacagcccg agcggagctg accggccaga actcgcgcga gtggatggtg
     9841 atcgtcgacc tgatccggcg gaccgacccc gccatgttga actacctctc gcggcagatg
     9901 ctgtaccgcc tgttctggca cggcatcacg gaggcccggg aaatcgtgcg ccacagcgac
     9961 ctgatcggcg acgagcacct ccacgaggag tcgaactcgc cgaccgagac gcagaccgtg
    10021 gagaagatgc tgaccctcag cgaagcggtg ttcgcgctgg ccgagaagca cctgagcccg
    10081 gaagacatcc tggcctccat ccagaagtgg atccaggaga agaggacgag cttcctcatc
    10141 aaggccgtcg acaacatcga caccacgctc gaggagatca tcgacgccgt catgctctac
    10201 aagaccatga cggagaaggc cgtcagcctg ccgccgacca cggagaagtg gttgcgggtg
    10261 tcgctgctcc ggcgcttctt ctcggatcac ctcgagtacc tgaagaccgc gaaggagaac
    10321 gtcgaggtcg cggatttcca cgacctggtc cagcacatca tctatcccgc cggaagcagc
    10381 gggaacctgg gggggaagag cgccggcctg ttcctggcgt actccatcct gtccaaggac
    10441 cgggaagcgg cgatggagaa cctgaaggtc cccaagacgt actacatccc cgcggacgcc
    10501 ctcacggtct tccttcacat caacgacctc gaggcgatca acgaacagaa gtacaaggac
    10561 ctcgaacagg tgcgcctgga gtattccaac atcatccacc tcctgaagtg ctcgcggttt
    10621 ccgaccgacg tcgtgaaagg cctgacggcg gccctcgacg atttcggcga caccccgctg
    10681 atcgtgcgaa gctcgagcct cctggaggac cgggtcggcg cctcgttctc ggggaaatac
    10741 aagagcctgt ttctcgccaa ccacggcacc cgcccggagc gcctgcaagc cctgctggac
    10801 gccatcgcgg aagtgtacgc ctcgatgttc ggccccgatc ccatcgagta ccggggcgcc
    10861 tgggggctgc tggacttcca cgagaagatg gcgatcatga tccagcaggt cgtcggccgc
    10921 cgcatcggca gctacttcct cccggccttc tccggcgtgg ccttctccaa caacgagttc
    10981 cgctggtctc cgcggatcaa gcgcgaggac gggctggtcc ggctggtgcc gggcctcggc
    11041 acgcgcgccg tcgaccgcgt gagcgacgac taccccatcc tgatctcccc cggcgcgccg
    11101 gaactccgcg tcaacgtcac gccggatgaa gtccggcgct actcgccgaa gaggatggac
    11161 gtcatcaacc tggaggccgg gcgtttcgag accgtgaaca ccaaggattt cctgcggaaa
    11221 cacggcccgg agatcccggg cgtcaaccag atggtctccg tcttccacga aggcgggatt
    11281 ccggccccgg cggggctcag cacggatttt gcgaacgacg atgtgatcgt cacgttcgag
    11341 ggcctggtcc ggaagacgac gttcatgaag cagctgcagg ccatgttgaa gcggctccag
    11401 gagaagatgg gccggccggt ggacatcgaa ttcgcctcgg acggaaccca cttctacctc
    11461 ctgcagtgcc ggagccagag cctgacgggc agcaacctgc ccagcgccat ccccagcgac
    11521 atcccggagg accgtatcgt cttctcggcc aaccgattcg tctccaacgg caccattccg
    11581 gaaatcacgc acatcgtcta cgtcgacccc gacgggtatg ccgccctgac cagccggaac
    11641 gacctgctcg atgtcggccg ggcggtcggc atgctgaacc gtctgctccc caagcggcag
    11701 ttcatcctga tggggcccgg acgatgggga agccgcggcg acatcaagct cggcgtccag
    11761 gtgacctact ccgacatcaa caacacggcc gtcctgatcg agatcgcgcg gcgcaagggc
    11821 aactacgtcc ccgaactgtc gttcggaacg catttcttcc aggacctggt cgaggcgtcc
    11881 attcgctacc tcccgctcta ccccgacgac gagggggtcc gcttcaacga ggcgtttctc
    11941 aagcagtccc ccaacatcct gcccggaatc ctgcgcccgt tcgagcacct cgccggcgcc
    12001 gtgcgagtca tcgacgtgcc gaagtccacg ggcggccgga tcctcagcat cctcctaaat
    12061 gccgaccagg tcgaggcgct cgggtttctc gcggagccgc tggacaggac ggaggacgcg
    12121 ccgcgcgcgc ccgaggcggc gcacgagcct ccgccggcca aggaccactg gcgctggcgc
    12181 ctccggatgg ccgagcagat cgcggagcgg ctcggcccgg cccacttcgg cgtgaaggcg
    12241 atgtacgtct tcggcagcac cgccaacgca tcggcccagc ccggcagcga catcgatctc
    12301 ctcatccatt tcgccggcac cgagcagcag cgcgccgacc tcatgacgtg gctgcagggg
    12361 tggagttcgt cgctcgcgga aatgaactac ctgcgcaccg gccaccaatc cacggggctc
    12421 ctggatgtgc gcctcgtcac cgacgaggac gtggccggca agaagggcta tgccgcgaag
    12481 atcggctccg tcacggatcc cgcccgcccc ctccgcctca agcaggacgg ctgacgctca
    12541 cccccccgga tcgtgctcgt gctcgacgaa ggaccgcggc gcgacttttc ccgaggacga
    12601 gcacgagggc cgctgtcgct gaggacgagt gcgagatttc accctggcga tgaagtctca
    12661 atcccgctcg aagtagacca gcactcgctg gcggacggcg tcggccatga tcttgatgat
    12721 cgctccgccg ccgatctgcg cggcgtcgag gacgcggacg acctccttca tcttccccgc
    12781 gccgggcacg atttcgagga gacggctggg tgtcttctca aagaactcct tgttgaggaa
    12841 attttcgccc tgttggggaa agagtgccag gtagaggatg tttctctcga ccagctcgtt
    12901 gaggaagtgc gtgccgaggg aaacgtccgg caccaggttc tcgtgcatcg ccacgatctc
    12961 gcacaggatc gaggcgcggt tgatctcgct gaacgtcacg gggatgccga ggtgcgggct
    13021 gccggacccc cagcggccgg gcccgagcag catcaccgtc tcgacgccgg ctcggcacgg
    13081 gccgcccgcc gcgccgcgcg cgcggttgat ctcgccgagc aggcgggcga cctcgtggcg
    13141 gtcctgcacg ggaagccggc tgtacaggtc cggggaaaca tagaccagcc ggtcgatccg
    13201 gttgagccgg ctctggccga tgaccgcgcc gcgcgccgcg atgatcaggt cctcgcggcc
    13261 caccttgacg tcgggcatct ccacgagcgc ggcgccctgg acctgcaggg gccggcactg
    13321 caacaggttg atcttgtacc ggccgtcgcc gacgaaattc gcggtgaact cgatatccac
    13381 cgggtgattg taggccactt gcagcacgtg aagaatctcg cgcaggtcct tgacgaaacc
    13441 cgtccgcgcc aggagcgagt cgaacgtgag aatccggtgc ggcttcccgc cgcgggcgga
    13501 cggcgcggcg tcctcggacg cgaacatctc gatggggaaa tcctcgtcgc ccgtgaccac
    13561 gtcagtgaag tagtcggaca cgagctggtt ggcctcgagg tccaggtagt ccatccgccg
    13621 ctgggcgtac tgggccacct cgtcgaagtt ggtctccggc cgcttctccg gcgcgttgag
    13681 cgccacgatc cgggtgtaat cgtcgtccga gcggttcacc gcgcgcgtgc cgaggccgaa
    13741 gacgaggcgg atgacgccgg ccttcgggtc gatctccggc gcccacgcgt acggattgaa
    13801 cgagaacccg acgccggcca tctgcgggta gaaattccgg ccgtacatcg cccccgagac
    13861 gcgcatgacc agcagggcca tctgctcgtc ccggtccagc atcccgcggt ccgcccggta
    13921 gctcaacgcc ttctcgctca tggagctggc gtagatcgtc cggatcgcgg ccagcaggtc
    13981 ctgcatgcgc cgctcccggg ggccctggtt ggcgcagtag acgctctcgt acttgccggc
    14041 gaacgagtgc ccgtagttgt cctccagcag ggagctggaa cggacgatga acggggactg
    14101 accgaagtag tccagcatct cctcgaactg gcgcaggatg tacggcggga actcgcccgt
    14161 gatgatgcgg cgccgcgcct gctcggcccc ctcgaggaac gtctcggggt tcctctgctt
    14221 ctggcggatc caccagacgc cgcagcgcac gagaaagtcg tagaacacgt cggaccccac
    14281 gaagaacgag tcgtgctcct ccatctcctc cacaaaacgc cggtcggcct tcttgacgat
    14341 ggcgcgcgac agcagcatgc ccaccgtctt gccgccgatc agccccgttc cgatcatgcg
    14401 cttgcgcacg tccaggatgt cctgcagggt caggtacctc gagatcaggc gctgcatgcc
    14461 cgggtcgcgc gagatgatca tcttgatgag cgtctcgaag acgtccttgc cgcggccggg
    14521 caggtcgggc cggtactcgc cggattggat cagttcgcgc gcgcgaatga agctcgtttc
    14581 ccagaagccg atactgctgt ccgacgcgag ccccgaccac tgcgacagcg tgaggatctc
    14641 cgagatcacc gcgctcgcgg tgacggcccg gaagctgtcg ccgtgccaga cgtggagcat
    14701 gtgcatggtg ggcgagtacc gatgctggac cttgaccggc cgcacgtaga ggtcgccctt
    14761 gtgccggtac gtgtcgagga aaagctgcgt ggtcgcctgg atcggcccga tcgcgtgcga
    14821 cgtgtggtag ttgcggtaca gggcgaagta cgtgaccgtc tccaggtcgt agaggtacgg
    14881 gcacgtcagc atgaagaaat tggccagcat ctggtcggaa caccaatccg cggccagccg
    14941 agagaggcag tcgaagacgt agaacactcc cggaccggcc tgatcgatga cggagtgaat
    15001 cgtcgcgatg aaggtttcga atccctgttc ggggtccaac tcatggacct cgatgccctt
    15061 gccggcggac agcagggatt gatggcgcgc gaatcggaag taaatcagcc gcttgccgtt
    15121 gcgaatcgcc gcccgaccaa agggctcgac ccactcctgg tactcctcca cggcatccac
    15181 ctgccagacg atattgtcgc cggggagaag tcccttcaga acctggtcca gggccggcag
    15241 cccggttgtg agcgtcggat caagcatggc ggccaaaatt cccttcctgg ggcggcggga
    15301 tgtggggtgc ggcatgcatg gcggttgttt cgcccatgag gatcgttact gtatgttctg
    15361 aagcagggca tggcaaggac ttctccatgc ggcacgtctc catgcggcag acttctcaac
    15421 gcgctcgcaa cccggtataa ggaagccgaa cttcggcaag agaggagcgc tggctatgaa
    15481 tgaaaaagtc aacacgtttg aggtggccca ggcccagttt gatgaagccg ccgatcggct
    15541 cggcctggaa tcctccttgc gtgaatttct ccggtggccc cagcgcgaat acaagttcac
    15601 cgtgccggtg aagatggacg acggctccac gaagaccttc cacgggtacc gcatccaata
    15661 caacgacgcg cgcggcccgt gcagcggggg gttgcgttgg catgccaacg aaagcctgga
    15721 cgcggggcgc gccagcgcgg catggatgac gtggcgcacg gcgctggtgg acattcccct
    15781 cggcggcgcg agcggcggac tggtctgcga cttgaggaaa ctgaccgagg gggagaggga
    15841 gcgcctcgcc cggggctgga tgcgcatcat ggcccagccg ttggccgagc atcgcgacgt
    15901 gctcacgccc gatctctaca cgaacccgca aatcatggct tggatggcgg acgagttcgg
    15961 ctctgtcatg ggccacgcgc ggcctggcgt ggtctccggc aaaccgctgg cgctgggcgg
    16021 gatcctgggc tggagcgatg cgacggcccg cggcggggtg cttgtggtgg gcgaggcgtg
    16081 ccagaccctg ggaatcgacc cgaagggcac gtacgcgatc cagggcttcg gcaacgccgg
    16141 gcaaaacgtg gcgctgctgc atcccgagat ccttggcggc ggacgattag tggcggtgtc
    16201 cgacacgagc ggcggtgtga tcaacacgga aggcatcgat ccgaaagcgc tcgtcgccta
    16261 caaattgaag accggcagcg tgaccggctt cccaggcgcc caaccggtct gtggggagga
    16321 cctgcttgat ttggacgtgg atgttttgta ccccgccgcg ctcgaaaacg tgatcacgcg
    16381 catgaacgca gagcagatca aggcgaagat cgtgtgtgag ctggccgacg gccccaccgc
    16441 gcccgaggcc gaccgaacgc ttcacatcaa gggcgtccat atcattcccg acatccttgc
    16501 cagtgcgggc agtgtcacgg tcggctactt cgaacaggtc cagaacgcgt cgaactatac
    16561 gtggcccctg gatagtgtcc ataaacagtt ggcgcgcatc atggcccacg cgtatcggaa
    16621 tgtcctcacg atgcacaaga agcagaacgt cccgatgcgc ctcgccgcgt acctcgtcgc
    16681 cgtccatcgc gtcgcggagg cggtgaaact gcgtggctgg gtatgaggga aagtctcccg
    16741 acgacgtaac cggaccgccg tgacttggtg gcgtctgggc cttaccgcga tcggtctgca
    16801 cggtggtgtc gccgaggccc cacatcggct atctgatccg gacagtgtcg ctggcgcccg
    16861 caatcatcgc agtacgcccc catccaaacg cgatcatact ggtccatgat ttgttgaacc
    16921 aaggaggcct ccgtcgtgat gatcccggcc tcgaaattcc gccgatgcgg gcttttagcg
    16981 cccatgccgg cccccgtaag gttagcgctg cccgtatagg ccaggcgtcc atcaacgatc
    17041 acggatttga aatgaacccg cgggcacagg atgcgttcca tgtcggtgac gagattagca
    17101 tagcggtcaa aatcgcgcct gaaattgggc cccggctcct tggcgtggag caggcgtatg
    17161 gccacgccct gttcgcatag cgatgacagc acgcccagga agggaatcat gtggcggcca
    17221 tgggccacgt ggagatcctt caggtcggct gtcgccagcc acagaaatcg ccgggcttga
    17281 ggcacctcgg cctggataac ccgccgataa atctccgcat cgctgacaaa ctcgatgtcc
    17341 gccatgcctg gattatgcct ggcccatcct tacattcaaa cccacaatcc agccgcaaag
    17401 cgtggagaat agcgaattgt cgccacctgt cgcagcctgt cgaaaaaaag agagttggtg
    17461 ctcggggggg gacttgaacc cccacggctt gcgccacatg cccctcaaga ctgcgccgaa
    17521 caccctagat ttaccaaata ttactcaaat aatgcggcat attgcaagtt tgattgacac
    17581 gttcttgaca cacttggcgc gtggggtata atggaagata ggagaaccac catgcgcaag
    17641 aaggaatcca agacaaccca ctatcacggc tttgcaatcc gcccgacggg attcgggacg
    17701 tggatggcgg acaactgcga ccggaccaag aggctgcgca agtgctttga cacacttgcc
    17761 gaagccaaga cctgggcgga gaacaaaagc gtggacctga aaaatcatgg ccttttggct
    17821 ctgaccttat ccgaccccga ccgccgggac gctgtggccg ccttgaccat tttaagcggc
    17881 gcaacgaccc ttgaaaaggc tgcccgagag tatatccggc ggcatccaca acacaacgcg
    17941 gaaaagctgt catggacctg ctggcggtat ctgcgccagt tgtaccggga gggcgctcgc
    18001 ccggtcacga tcaagaatgc ccgatggaag ctgcgcctat ggtgcaagac cgagggcgag
    18061 aacctgacgg cgggcataag ccggacggaa gtggatgcat ggattgaacg gcggggctac
    18121 aaggggcaat cccgccgggg ctatgaacgc cagttgaacg ccctgctttc cttctatgcc
    18181 gggacgcgga agcgccggga attggccgac gccaccccgc cccccgtgtt caccccgcaa
    18241 gacgttgagc ggctgatgtt cgccgccgaa cagcatgacc ccggcatggt cgcccccttg
    18301 accttactgc tgttctgtgg cctccgccct tatgaaatga tgcgcctgac gtgggacaag
    18361 attcaccttg cggaaggtga agtgatcgtg ccgccagagg cttccaaggt ccgccaagcc
    18421 cgagccgtca agctggagcc ttgcgccttg cgctggctgg ctgcaagccg gggaagtggc
    18481 aacgtagccc ccggccagta tgaactccgt cgccgccgtg aagccctgat ggaaaaggcc
    18541 gagattgaag catggccccc ggacgtagcc cggcatacgt tcgcaagcgc cctgtacgcc
    18601 ctcaagagcg atgccgccta tgtcgccgcc caactgggcc actttgggag cctgggcgtc
    18661 tttgcccgtc attacaaggc tgtcatgcga caggccgacg ccgaacgctt ctggactatc
    18721 gagccgcaca agaaggaaaa tgtgatggtg ctgggggcga gttgaacagg gtcaagcaaa
    18781 acggaatgaa gggaagatgc cgcatgaaag aaagagatta tgaaagaatc ctcgcctctt
    18841 taagcccaga gcaacataag cgacttctgg agatagcaga actggatgcg aaagtgccga
    18901 agcaggaaaa gaaggtaaga cgctttttca agcagagggc caaaaagaga gaagaagaaa
    18961 taaagaaatt agtcagccta tcacccaaag acgtactgga acaatgtcgg tgctggattc
    19021 tggatttctt tgatgaccgt atcattgaaa aacagtttgg agtcactggc ataaagagtg
    19081 cttttgcccc tcaacagaaa aatactcatt cacaatcctt ggaggactta cgccaatcct
    19141 tttgtagctg gctatcgcgg ttaagcgaca gaacgcagtg gcgctatttc actacgctat
    19201 tgaaataccg ggctattcca acagagtttt ttattgagcc tttctttcag tacataaagg
    19261 agttggccga tcatcccgaa aaagccgcgc ggtcagcagg taaagcggcg attgtctatt
    19321 ccatgaccgg gcgcgggggt tcaatcaagg gggggcgctt gaatagactt caatcaagcg
    19381 agattcttgg tgtttgtcta aagaccttcg acggtatgcg caagcagaca aaatgccccg
    19441 acagttggct tggcaggaaa gaaacaaacg agcgcgttgt cacttatgac gcagaacaag
    19501 tttatgactt aaccattgag cgattacaca cgtcgttgtc agtcgaagcc gaaaaaaaac
    19561 gtgaggccga aagaaaccgc cgcgaataaa tggcccatgc tgggcgcatc acataagaaa
    19621 ccttggcgca cttaggcgtc aagcaattcc ccaactcctg cgtgttagca gtcaaaacag
    19681 cctgataatg cttcaagttg agttgacgtg tccctctcat tattttgcac ctactctaac
    19741 tctcgcgctt ttagccattc ccctccgcca acccgcccat agttagggca taatccgttg
    19801 ttggcaagcc agatgctact agttgcaggt agcagataca tgatgcgact ttataacgaa
    19861 gcgtgcatgt ataggccgca agcgcggcga agtgcgcgga ttggtgaaga gctatggatg
    19921 cggttttgtc tagcgacctg atgcgaccca acgaggccgc aagtttcctt cgggtgtcca
    19981 ggcggtgttt gtccgacatg caacggaggc ggttggtgcc ctacgcaaaa cttgggcaca
    20041 gactggttct ctttcgccgg gcggacctcg accggttact tacgcggcga acgattaaag
    20101 ctatcggtgc gtcatgatcg ccgtcgcccg aagcgcgccc ctggctcaag cccgccgtgc
    20161 cgtcgaaacg acccatgaat cggtgggctt gggcgtgatc ctcccggtga acccagttgg
    20221 attgaccccg gcgacaaacc ccgatcatgc cccgattcaa ggccgtggcg ggcgcgaaaa
    20281 cgaccctgaa aaggcaaagg ctgtcgagaa gcaagcggaa gccatgcaag ccgtccctgt
    20341 gaggctccgg ggcttggttg ccagagcttt tgaggggaaa tcccttcgtt cctgcgtatc
    20401 ggcaaaatgc cttgagtgcc tagggtacga ggaccgcgaa gggatcacgg attgccgtgg
    20461 atacacctgc cccctttggc gagttcgtcc ttaccagaag aaagcgggac aatcatgagt
    20521 gtcgagatct tcaagggtag cgagtccgaa cgttacacgc ccaggacaaa agccttttgc
    20581 cgccgctgca atcagaacac cccgaccgag attcacgagc ttccgggcgg gcttggaaac
    20641 tgctgcgcat ggtgcggttg ttgtcgcaag ggaaagccct atttcagcaa ggccgaggcg
    20701 gattcactaa agcggctttt cgccgcagaa ggtgaacatg agcaagcgac accttgttga
    20761 gccccgcgca gagcttgaat cgtacatacg caaacacttc cccgacctga ccggtccgga
    20821 aatgaacgtg tatttcaacg ccctgcccca tcttgaaacc atcaagccca agtccgctta
    20881 tggtgccaag gtgttggcct ctgaagatga ttggtgcatg gcgtcggggc acaaccggcg
    20941 aactgtacac aaggctctag tggaccttgc agcccgaggg cctatcagtt taacgcccgg
    21001 cgacaatggg cggaccaccg gcaagcgcaa ggccgcgatt gtgcggcgaa actctattgc
    21061 ggagattcaa acagcctgcc cgcgtgaagt tctgacagca tttgttcccg cagatgccga
    21121 acagctttgc gccattctta acgaacgcgg catcccttgg catgggcaaa caatcaagcc
    21181 ccgatggacg gtgcagcgaa caggccgaat cggttacggc ggctcgatca tggtgcagcc
    21241 tggtcacaat accaaggccg cacggattga agcattccgc gccagcttgc gaccgggtga
    21301 acggctgatt gaagcggact tctcatcggc ggagccttcc cttctttgcc atgagcttga
    21361 acaaaataac ctgatcccgc ccggcattga cccggcaaaa atctattccg agatacaacg
    21421 cgctgcgaag tcttcacgcg acgacgcaaa gacccgtttc cagaaaacgg tctattcgcc
    21481 atatcccaat ctgtacgtcc cggaagcgtg gaatttggag caaggccatt ttctccggga
    21541 agtcatttcc ggggcggaac gctaccgaaa ccgactatgg gaccaaggca agccgaccaa
    21601 ggaccaaccc cgtcatgttc acaccgctac cggccgcctt attcaagggg tgtcacgcga
    21661 acggatacac agggggaagg ttctcgcatg gaggctacaa ggaaccatcg cagacatctt
    21721 cctttcgatc ataccggccg tgttggaggc cgaacagcgc ggtgaatgcc ggttgtttct
    21781 tccgcttcat gatgccctct atgtttccgt tcccgagggg tcaacattca acccggcccg
    21841 gctcatgcag gagaaagcgg cggaacttgg attgcctttg aaggtaacta caaaagaaca
    21901 cgggcaggga ggggggcaga ggaggcgacc agggggcaga cgggacagga caggactgga
    21961 atagggtgtc tttttacact caacaaggca tgaaaaggcc gttattttgt gtgcaataat
    22021 gcaccctatc aatggggcgc aggagggtgg ctatcccgat atggattgtg ggcaacacct
    22081 tcaaaaccac cggtatttac tgaccctatt ttggctttag ctgcacgttg ccatgtagaa
    22141 acaggatgcg agcctggttc aggcatgccg ccattttgca ggagtgtaat tgtcgtcaat
    22201 gcgtgtttat aattgtcgtt gaccaccgtc ccatcataac gttatgaagc taccccaccg
    22261 ccccgaaaag ccgcgaaaat ccgtagtgcc gatcctgggc caatatccac ccctgccttt
    22321 tgtttacagg gttatacgcc ttataattgt caagtaatac ccgccagaat ccgtgagggc
    22381 tttacagaag ttcacgcttt attaactggt ctctttgcgc ctgtctgcat ggcaccgggg
    22441 aatagtcgcc gagtttaaca tcgggttgtt ctgcctgccc gttgcccgtc cttcttgcca
    22501 aaccctggca ccaaggcgta atctgcttct gacgctaggg gtgatacatg aaccttcgac
    22561 gcaccggact gcttcttacg gttgtaatgc aggcgcacct ctcaatggca gagatgtttg
    22621 acatagaggc ttttcgcagc aatggcttgc tgatgtggac agacatttat agtaacggcg
    22681 tttactcaat tcaatgggca tcctcgccaa ccgatgaatg gaacaaggca tggtccaaac
    22741 tggaatccct gaccgcaacc ggcggaacaa tgtcagtaaa tatcccgatg tactttcgca
    22801 ttacttatcg tccattcttt gctccacata caaatatcgt tcttgttgcc ggaggtggac
    22861 aaccgctagg ccccccgtat gatttttata tgagcaaata cgaagtacag aattgtgagt
    22921 gggttgattt tctaaacgac gcccaaattc atacgaacga tattcgtgga accaatatgt
    22981 tcttcgcatc tggtggaaat gtgtacatga attctagcat gtctagtgat gaaatactgt
    23041 tttccattgc gagttccgac ttaatctatg atacagggaa cccagttggg gcgagataca
    23101 gcgtgtggtt ggttccgagc aatcggccca tcacaggtgt gtcgtggtat ggctcgctga
    23161 agtactgcaa ttggttgacc cttgttgctg gacggggcga agaccagcga tgttattctg
    23221 aaggcacaag cgggtcggct tggcatccaa gcggcattgc ttatgcagat tggtatgaca
    23281 actttagtcc tccagaaaga ttagcttggc tgaacaattt caggggtttt cgactgccga
    23341 tggatgatta ttgtttaagg gcaagctatt tcaatgagtg gtacaaggcg gcggcgtgga
    23401 atggccagac aaatactaca tatgcatatg ggcgaaattc aattgatggg caggacgctt
    23461 gctggatgag cagttcgcat ccttatcgaa tgtatacaat tgggaccacg cccgtcggct
    23521 attacgacgg gacaagtcat ggcggctcat ttcagacgcg agcaagcagt aatttcttcg
    23581 gcctttatga tttaagcgga aatgttgcgg agtggctttc agacgcttgg ggtccaaatg
    23641 acccagacat gagaacccgc ggaggattct ggagtgcaaa ctttgattat gcagccacag
    23701 attatctcga ctgggatcgc cagaaatcta cttgtgcaaa ctatctagga ttcagaatcg
    23761 ttacaacggg gccttgaact ggtctcgata cttcgcaacg agcaccagcc ccgcccatca
    23821 taacgtgatt aaccccgatc atatattatt ttcggggtga atgacctctc cgctcgccac
    23881 aagaccccta cggacgcggc aaccctgccc ctgcgctaag tccgaggaaa agctggcagc
    23941 ctacccggct aaatgacggc atggcgggat tggggcgggg atgaatgttc accgccacga
    24001 catattacgc gcaaatattc atctcgatac tggtcctgca cataatcaat cctgaccagt
    24061 aatccgttgt ttcggcttgg tctcacggaa cagcttttcg acttggggat ttgcggcatc
    24121 actattaaac agaacgtaat aagtgtgacc ggcgccaaga tggagccgag agtatatgaa
    24181 gtcaatattg cctcggaatc tgctcgcttt cgatacgact tcgtacctgt acttccatag
    24241 tcgttggatt ggaagccatt cgcttgtcac tatccgtatc gtaattcgct gccccatcat
    24301 ctcaacactt cccctggaga gcggcctggt tgtatgcttt cttcttacat tcacacgagt
    24361 gagcggcgca ttcaaaggcg ctttagcaga cgctttggct gctttgtcca cggctttgtt
    24421 gtgctcgtcc tttgagtgac ctttcaccca ataaaaatgg acgggnnnnn nnnnnatctt
    24481 cctgatgagc tttacaagtt ctttccattg ctccgcatta tcaaccggcc gaccattttg
    24541 attgcgccaa cgatttcctg accattggaa cagagccctt tgatagttgt ctgtgacgta
    24601 acgtgagtcg gagtgaatgg ccacctgtct aatgtagtcg ggcaacgtgc gcttcttcat
    24661 ttcctttagg gccataatgc cagcttgaag ttccatctga ttattcgttg cccccctgta
    24721 tcccagaaaa ggaatgtctt ctgtttcatc ttgcccgacg gaattggtta ggatgaatct
    24781 cacgccaatg ccacccaccc tcggcgaatt ccgtgaagag ccatctatgt agatattcag
    24841 ggcattctct ttcgtaatca ttttatgacc tgtacagcgc tctaggataa tttcccttgg
    24901 ttgcaatgtc tattccgaaa caacgtgtca gcctatcatc gcgcaattaa tcccgaccct
    24961 ttttttgggg ggaataacaa cttgagcgcc ccgcgaaggc cccccggcgg ggagaacggg
    25021 cacacccccg ccctccccct gacgcctggc gctgtccctt ggcgcggttt gcggctgcga
    25081 taccggcccg gcttggcgcg tcccgtcact gtgttcctgc cgctgtgatc ttgtgaccag
    25141 tccgccgccg tccagtttca cccgtggcct ttaacggctc cagcttgccc cgggcggggc
    25201 tgattatttg tcgggtgtag cgccgggttg aatcggggct tgtcgggcgt ttcctgcgtt
    25261 tgagaggggc aggcataagc ggcctccgcc cggctgtttg acacgggctt gacacaatcc
    25321 gaagcattac cccgcgtttt cgtgctagat tgctctaatc ctgcataagg ccataaacgg
    25381 ccacaacagc taggataata cagtatttac agggggtttt aaactggtgc tcgggggggg
    25441 agtcgaaccc ccacgccttt cggcacatgc ccctcaaac
//
LOCUS       DAOOTK010000036        25328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1364_length_25328_cov_5.903059, whole genome shotgun sequence.
ACCESSION   DAOOTK010000036 DAOOTK010000000
VERSION     DAOOTK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25328
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_25328_cov_5.903059"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK634_06075"
     CDS             <1..245
                     /locus_tag="PK634_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97338.1"
                     /translation="VLVLTSETNQVGVGVVATDGGDLVTYIEHESPSITPEERAARIA
                     AKVRDHNGKKQAAKDGLNGQLQQRLENLERLLGVRE"
     gene            249..512
                     /locus_tag="PK634_06080"
     CDS             249..512
                     /locus_tag="PK634_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97339.1"
                     /translation="MISKTIAVSAGSLAALASAAPASPEALEAVSKWPVTVVLGAVCC
                     FCVYLMYRQGSDYRASLDKIAGALTKLSENLAQRPCIRDPHND"
     gene            516..989
                     /locus_tag="PK634_06085"
     CDS             516..989
                     /locus_tag="PK634_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HOW97340.1"
                     /translation="MNTNAILLKVPDPEGRFDGAFRFEYVNGRDWAVAGDISYRTQAG
                     PVSTVRAGFTTDFATIPPFLWPFMPPAGDGRNFYGLAALWHDWLYWHGAVEGRPITRA
                     YADSIFLELMLYLGVSRPLARLMWAAVRSFGWYLWRKYRRHNENRIDPVGRPAAL"
     gene            946..1365
                     /locus_tag="PK634_06090"
     CDS             946..1365
                     /locus_tag="PK634_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97341.1"
                     /translation="MKIASILLVALLLCNGCATALTNQLLQRQSAVRVEVDGKTIMAG
                     VDLFALDEVVEHPFIHGFAAVIDLGAGYLAYDALEDSGGSSSTHSTTAYTIQTGDNSP
                     VYFMDNSTVNNNQSSTPAPAPAEDSTEETAQRARRAR"
     gene            1365..1487
                     /locus_tag="PK634_06095"
     CDS             1365..1487
                     /locus_tag="PK634_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97342.1"
                     /translation="MKLLAVILAALMLAGCGTPKGYDWDTTPDHDGEEYVGGAP"
     gene            1484..2401
                     /locus_tag="PK634_06100"
     CDS             1484..2401
                     /locus_tag="PK634_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW97343.1"
                     /translation="MKLLCVDSQGERIHAPYRDLLPDIELRGTPTPEKWRDTICHEHG
                     SLCGWLLALPAQAAILAGELIEKVELVFCRIFDQNARFIPGSEERILEVIREERPDVV
                     SRSWGQWDLDDTLGEFYAQVEWADWAERYAALQAEIGFLDVGAAGNDDDNDKDDDVVY
                     PQRLLNSCCIIGAAHRSGKPAPWSGDGAGVLCVMWGDRVYSPDATGRWRLWSGTSAAT
                     PKFAGAAALRRLQTSALKLAVMHEATRPTKAVYANMTHHAKWGFGNWEEAWQKYARLM
                     PQDLMPPPAPKGLRSLIAPIEFFDFSRPC"
     gene            complement(2413..2655)
                     /locus_tag="PK634_06105"
     CDS             complement(2413..2655)
                     /locus_tag="PK634_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97344.1"
                     /translation="MQGKKVVRLPRVERGTFSSGGPENEKIAFFAVFHFPEMKGAFQP
                     CKQRTKNTIAGQNKARLRTVRNRGQVTHYCPHKKRR"
     gene            complement(2726..3538)
                     /locus_tag="PK634_06110"
     CDS             complement(2726..3538)
                     /locus_tag="PK634_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOW97345.1"
                     /translation="MDMQAIQTFVSTTLTQLAVKILAAIAFWVIGRWLIGHIVALSRA
                     GMNRSKIDPTLTKYLGSIIAIALNVALALGILGYFGIQTTSFAAMLAGAGVAVGAAWS
                     GMLGNFAAGAFMLVLRPFKVGDFVQIGGLVGTVHELGLFTTTLITPDNVSTLVGNSKV
                     FSDTIQNFSIRPVRRVERTAQLAGSVDPLDAIARFKAAVAQIPNVAKDPAPEVNLLDI
                     NLLGAVISVRPYCHTDHYWQVYFDTNEAIIRVCKEAGWPAPTPTQIMKTTAA"
     gene            complement(3720..4403)
                     /locus_tag="PK634_06115"
     CDS             complement(3720..4403)
                     /locus_tag="PK634_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOW97346.1"
                     /translation="MINPDVFHFLNQLKANNNRRWFVQHKAEYAAARAAALETIDHVI
                     AGMARFDPPIVEQDPEDCLFRINRDVRFSRDKSPYKAHFGAFITDRGRKVDRAGYYLH
                     VEPGDSMLAGGLYMPPALPLRAVRQAILRDGRPLRRIVSRPNFVKLFGDTLPGRRLKT
                     APRDIPRDHPDLDLLQLTSFEVALPLKDARLRKKDFVEYAVGVFEVMHDYIAWLNRAL
                     DSFAPDRSA"
     gene            complement(4457..5920)
                     /locus_tag="PK634_06120"
     CDS             complement(4457..5920)
                     /locus_tag="PK634_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97347.1"
                     /translation="MSEHESTDTRAGGKPRFYYQKVAAQLKAGWSARKEIAEIRRRQQ
                     AVPTAPEEFGRNSARIAELQPAVRQMNKTVAIIAVLALTLAWAVTQSGGARMDVVAQI
                     GTGRALIARFAGVSSARAALQATLKQLAPAFDRMPGANRGFADQDDRMGGAWFEASQQ
                     GEPVAGFMWACVGPRGATVTVIHDRRAAAPQSFRQLAAAISGAAETGGGAAPARSAPM
                     QQAAFPDGSGSVQLPADWQLVRASRGQGRAVGPRGQVAFLNIGLQVWTPEKAQQAQMF
                     GGNYPFVAPYVPDPVQAFVNLAPALSQSEMQAGSPGFTVADVLGSERMDFQGYPAALV
                     HLALDFQGHRHQALACVMCSPPAGYGAMDWVFTMTLVTTPEELAAQDFPAMLKILQTS
                     QISSGEVGRRLSSAAETVRSIGDIIRRGEGGGGAGDNRQAPQDAFDETIRGWRTIEDA
                     VTGTRRDVALGHSTEIVDELNKHYGPDRFREIPLGTN"
     gene            6001..6360
                     /locus_tag="PK634_06125"
     CDS             6001..6360
                     /locus_tag="PK634_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97348.1"
                     /translation="MSATNAEGSAASADQAFATPVFFARGDFNGDGVVAQDEMDSVFA
                     GYWRDNPTVISNLLGAGSETVQLAVSNMANWGLAVETSTDLVTWSTLLDSAVPVFQFF
                     DPDATNGSPRFYRLRAP"
     gene            6464..7384
                     /locus_tag="PK634_06130"
     CDS             6464..7384
                     /locus_tag="PK634_06130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97349.1"
                     /translation="MDASRRMAEDNSRRMEPPGPRVHIITLNWENEPVTARLLAALRE
                     LRYPNRETVVVDNGSADGSADRLAAAFPEAVILRAGRNLGFGAGNNLAIRRALEAGAD
                     YVWLINNDARPAPDALDALARAAEAAPRAGAVGAAIYHEDRPNALQAWGGGTVARWLG
                     WARLATRPGAPVEYITGTSMLLRCAALREAGLFDERFFLYWEDAELCARLRRAGWEMA
                     VAGEAKVWHASSATTGRDAELRMFHITRSLALYLGARSRAPGVESLVATLFQAAGKLV
                     RGNRAAARGAWLGRRAGLEELRAARAPGRS"
     gene            7389..8621
                     /locus_tag="PK634_06135"
     CDS             7389..8621
                     /locus_tag="PK634_06135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW97350.1"
                     /translation="MPGLASLAKSALALPGRAARGAAALARRALAPAPDPDRLLIFDD
                     AFPHLLSAFRVAEYNEVLRAFPRARVFSTGACFRSFKDRRSFAGVRREYETRHPEFRG
                     RVSKFHPLLDFRGRLAYTIFLHNILFFLPVIEKRRLPFVFTLYPGYRLRVPETTAELR
                     RVCGAPGFRGVIVTQPVTREYLLEEKLCPAERIEMVYGGVVPADVLAAASVPRRRMGR
                     DKDTLDICFVGFKYVEGDLFKGYDVFVEAARRIARAIPAARFHVVGTFGPGDADSAEL
                     GGRIRFYGPQPTAFFPEFYARMDLILSPNDLPRCIPGAFNGFPTGCCAEAALSGVAVF
                     CTDPLGQNVGLKDGEDIVLINRRPDDIAEVVGRYATGYDALCRLGERGRAAFRRIFDL
                     RTQMDPRLKVIARALEEG"
     gene            8626..9924
                     /locus_tag="PK634_06140"
     CDS             8626..9924
                     /locus_tag="PK634_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOW97351.1"
                     /translation="MDTDAPKKPYRVLVFPGSTGIALEIQKALAGCKEVELFSAGVDG
                     PTHAPYLFARHFTAPRVDQPGWVEALNRLISEQRIDFVFPAHDDVVEALALRAGEVRA
                     RIVSSPPETCLVCRSKRETYKRLKDAVPVPVVYDRPEAVPAYPAFLKPDRGEGSREAQ
                     VVRHRRHLEVLLEQSPDYVVMEYLPGREYTVDCFSDREAGLLFCGGRERVRTKAGICI
                     HSRTAENPAFRRHALAISAVLPLHGAWFYQLKEDREGRLKLMEVGPRIAGTMSVHRVQ
                     GVNFPLLSLYEQLRVPVEILRNPVEVEVDRVLVSHYRPVVSYDTVYVDLDDTLILRGR
                     VNLPVVRFLYQCVNEGKRLVLLTRHAGDPEATLRRRRLAGLFDEIVRLEAAESKADFV
                     REPSAIFIDDSFRERKAVGERRGIPTFDTSMLELLFDERA"
     gene            9942..10697
                     /locus_tag="PK634_06145"
     CDS             9942..10697
                     /locus_tag="PK634_06145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOW97352.1"
                     /translation="MSTPKLSAYYVSDEETQGYAEILRALRDCPIPDDERLKHLPLFL
                     NRSLLGHILFMDELYRLILPVHGLIMEFGVRWGRNLALFTMLRSLYEPHNFSRRVVGF
                     DTFGGFPSVSPKDGSSKSIKVGTLAVTPGYEEFLAGLLGSHEKLAPRSHLRKFELVKG
                     DATETLPRYLKDHPETIVALAYIDFDLYEPTKKCLELIRDRLTKGSVLAFDELALPEF
                     PGETAAFQEIIGCRHARLRRSPLVPYQSYLVVE"
     gene            10700..11986
                     /locus_tag="PK634_06150"
     CDS             10700..11986
                     /locus_tag="PK634_06150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW97353.1"
                     /translation="MAGRDPARGFVSRAAAALRWAGEGAAALARRPFEPPPPADRLAI
                     FDDIFPCPFSSFRLAEFTACLAAFPSAQVFSTGTALRMLGGTASFRDVLAAYARDYPA
                     YAPRVAKYHALKNLRGRAAYAVFLNNAQAFLAAIERNRLPFAFTLYPGGGFALKQAVS
                     DEKLRRVCSSPFFRKVIATQPATLDYLVGGGFCRPEQVELIFGIVLPLNLLARAAPPR
                     KRKGRDKDTFDVCFVAFKYMPGGVDKGYDLFVEAARILAARRPDIRFHIVGPYDAGDA
                     DVAALGDRIRFYGPQPTGFFPAFHAGMDLLLAPGPAFARSPGEFDGFPLGACIEAALC
                     GVALFSTDPLGQNRYFKDGEELVVIPRDSDGIVDLVDRYYRDYDRLCELGRAGQAACR
                     RLYGWEAQMAPRLRILAELLGVEPPRLDAEARAELP"
     gene            11983..13566
                     /locus_tag="PK634_06155"
     CDS             11983..13566
                     /locus_tag="PK634_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97354.1"
                     /translation="MTPKPNIVLVGIAGTPDDFLLSPYALKAWLAAKPAIRDGAEIEI
                     LSYPFVPAGQRAEAAERILRDVCAREPRLAGFACYLWNRETVDLLAAGLKARRPDTLV
                     ALGGPEICEEEIRAGRFDGASADFLVFGEGEKPLEALALHLLGARPGLDGVRGLARRE
                     EGRWVTHGADAIERLDQAPSPYLTGAIPDELLARPGIRANVESQRGCNFRCAYCFYHK
                     DFRGIRYRDPGAVVEEMALVDRRGIRMCRITDANFISDREHAKTILRGLVERRLQLSL
                     FAEILPQFVDEELAGLFGEFVRCAPGNVLMLGIGLQTTHEESLRAIRRAIPLRHFERA
                     FDLLAGQGAILKTDLILGLPRETRESYFRTIEFIAEKLRRGTHFLSLAVLRVLPGSDM
                     AEIARREGLVPDTRDAGRFVYETPTMPRPHMLECLRLNAAAFRVLSTVHMTQGPRIRD
                     LYFEAKDRLGVPHVRLLRRLARDFFDYLQARRTDFVKPDFPNAEDYASKDIRRDIPDD
                     WLAERLARLGEAGLPETES"
     gene            13573..14403
                     /locus_tag="PK634_06160"
     CDS             13573..14403
                     /locus_tag="PK634_06160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020327198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97355.1"
                     /translation="MRTCNYLPLGRMARAVRGAWRMLLAAGGTRECPACGRRIRRFQP
                     FPKEAADLLARHRFRLPWKSSAWETFNREEYACPFCDATDRERLYALYLRERLAPGLG
                     DGLRLLDIGPSRPMTRFVRRRLRAAYRSADLCNPEADDRVDLEDMRIYRDGEFDVLLC
                     SHVLEHVPDDRRAMTELFRVLRPGSWGIVMTPIPLGVEKTDEDPGVTDVAERLRRFGQ
                     EDHIRIYAGADFTERLQKVGFRVTKLGRGHFGPEAFERCGLTPQSALYVVDKPMGAGA
                     "
     gene            14400..15149
                     /locus_tag="PK634_06165"
     CDS             14400..15149
                     /locus_tag="PK634_06165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97356.1"
                     /translation="MTADLGHILEYWDREDVESMYDKHLLNAEIELIRRRLAPGLKIL
                     DAGCGEGEGTLAYSMLPGAKVHAVDFSATRLRKAAACVAGRDNVTFQQVDFLKPYRLD
                     RDFDAIVSQRFLINLPAWELQGRVLLELMDHLKPGGRLLILEGSQPGVDELNGFRAAC
                     GLEPIPVKWHNLFFDDEVLVAFMRERGHPLAEVDGLGAYFLLTRGVRPLLDAKLDWNC
                     EFNRRAAAPAVESALRLGARFSRLKLWVFRK"
     gene            15146..16351
                     /locus_tag="PK634_06170"
     CDS             15146..16351
                     /locus_tag="PK634_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOW97357.1"
                     /translation="MKPSLQPLAILGGRPAFAERLYVGRPNIGDRGRFLARVNDILDR
                     RWLTNNGHYVHEFEKALAGLTGARHFIAACNGTVALELAVRALGLRGEVILPSFTFVA
                     TAHALQWQEITPVFCDIEPGSHTLDPARVEERITPRTSGIIGVHLWGTPCAVEDLAAI
                     ARRRDLRLVFDAAHALGSTRGGRPVGGFGDAEVFSFHATKFLNTFEGGGIATNDDELA
                     ARLRLMKNFGFAGYDEVVYLGTNGKMSEVQAAMGLTGVESLDEFIACNRRNHERYRAA
                     LAGLAGVRLLEPAQGERHNFQYVVLEIDETAALARDELLRVLWAENVVARRYFHPGCH
                     RMEPYRTLFPDVAARLPVTERICRRVLVLPTGTAIGEAEIDAIGSILRAALARGAEVR
                     AALARSPGP"
     gene            16348..17316
                     /locus_tag="PK634_06175"
     CDS             16348..17316
                     /locus_tag="PK634_06175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW97358.1"
                     /translation="MSDPGGPRPRVSVAMITFQHERFIAHAIESVLMQDAGFPFELVI
                     GDDASTDHTREVAGEYGRRHPGRVRLLTAESNQGVARNFVKTLRECRGQYVALLEGDD
                     YWTRPDKMRKQADFLDAHPEHAACFHPVKVRADGEAGTRHVQRVRPPRGGYGLEDLLR
                     ANFMHTCSVMSRNHLIPDWPEPFLDLKMLDWPYHILNALRGTVGFLPEAMAVYRLHAA
                     SAWSAGRLRDRIAETRRMLERVNAHLGGRYDRVVRRSLAECDCQEAMQFAREGNRRAA
                     RPLAASCVRAYRSGNGPPLAALLALGAVMAWPALYRLVLAKKPLWR"
     gene            complement(17305..18444)
                     /locus_tag="PK634_06180"
     CDS             complement(17305..18444)
                     /locus_tag="PK634_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOW97359.1"
                     /translation="MRIGVLARGLAVRVGGVTTFLENVIPLLPRLDPANRYVIYHHSA
                     RHGGAFPGAEEHRLRGPGTLWQENVALPLALRRRPVDVLLCAKNFVPAFLPRGVKTVA
                     IVYDLLYYPVRGASIDEYKPLDVLAFRALFPRGLRRADRIIAISESTRRDLLDLFPLD
                     PARVRAVPLGVSPPAPGLLAEEALRSVRSRYGLDRPYFFYCGSLSPRKNAVRTLEAFA
                     RIRDRVPHNFVVTAGKSWKDRAVFRAVERLGLRDRFRRLGAVPAADLPALYRAADAFV
                     YVSLYEGFGLPILEAMSCGCPVLTSRASAMPEAAGDAALLVDPADVGAIAEGLERILK
                     DRALADGLRVRGARRAAEFTWEATARHIIEVLAELRPADRPPLSP"
     gene            complement(18441..19922)
                     /locus_tag="PK634_06185"
     CDS             complement(18441..19922)
                     /locus_tag="PK634_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97360.1"
                     /translation="MDEPTREESFRAVSGAMAIGAGQAAIILVSLCTTMLMPRLFGAE
                     DFGRWVLFRSVISLAMILCVSGSSELMGRYYVALRAEGRDADAGRLFKLVFAFRLALA
                     GLGALASGLLLAASTRVALGGEAAVLSGASVLLLVIGMSFHAVLYSERRMPRVAALNL
                     LNASLVPAVVLAAYAAAGFRWVPRACVLGDALYALAAFALARGPMQWAAGWPPRARWP
                     EYGRFVATAGFSNALVSVYGNLPPYLMAGAGMDAAQIAFMGLAVRVGDIVRMTLVSVG
                     GALFPSITWVYEREGLDRALRWQGIATRFGAVAALAAAGVFAAAGGGLVRLVWGAAYD
                     GAVPAIGLVLFSVVFLWIGFQHGWLGMLLKSATPFAAGSLALVLSFPLLFRVLGPAGG
                     ARGVAQAQFLCAAGFMALVMTHVRLGWKQGLPVARLAAPALAAAAAWPLGFGSRLGLP
                     AGLAAAALWAACFAAVTFLSGSLRWAEVAEIAGRLRRAPGKPA"
     gene            complement(19992..20876)
                     /locus_tag="PK634_06190"
     CDS             complement(19992..20876)
                     /locus_tag="PK634_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97361.1"
                     /translation="MNTLAFTLAGVRFQLRGARDDLVPRLPEAYLKFRAGPPSGPAQA
                     EYRVLDPRDASLAPVETGALLWQSETWRLGRTGDGRLGIELQRVADGQWIPVVNAQPD
                     FSRGDIRPLYGRMTAPSEYALNHPYDQTILLNRLLHFGAGVVHACGIEDRGRGLIFAG
                     RSGAGKTTLARLWRGLGATLLNDDRVILRNSAAGVLLESSPWHGEDPQVNSATVPLRA
                     IVHLNQARENRLRRLEGNEPLTALLATTVAPFYSEHGLEQLLDAWTAVTARTPSYRLD
                     FTPDARAVELCRAEFRDA"
     gene            complement(20886..21053)
                     /locus_tag="PK634_06195"
     CDS             complement(20886..21053)
                     /locus_tag="PK634_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97362.1"
                     /translation="MDKQNEQAPKKYTKPAVKRIKLEDKRVVSMAACKTGPDDVPCQD
                     EFGGQGFDYGS"
     gene            complement(21291..22370)
                     /locus_tag="PK634_06200"
     CDS             complement(21291..22370)
                     /locus_tag="PK634_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97363.1"
                     /translation="MSCSDCTIPEIGSADFLDELARRPAGEDTLPMSGSIELTLRCNL
                     RCRHCYIHAPGAADGEMDTARARKVLDALADAGVLSLLVTGGEIFLRRDFREIYLHAR
                     RRGFLMTLFTNATRVDDGLADFLAAQPPRRIEISIYGHTAETYERITGGPGSFAAFRR
                     GLDRLVARDLPVYLKSMVLRSNVHEFAAMKAWAESLGRPFRYDAIVNARLDGGTEPFA
                     ERLPPEEAARWQVEADAEPRSPAEWRGKTEGWGPDPRLFRCGAGRVTFHVDPRGMLHP
                     CLMWRRDPYDLLDGSVAGWRRHMAELLRRRAPADSECASCLDRTMCLNCAATSAHEAS
                     APGLPAAYYCAVCRARATRRPSAGK"
     gene            complement(22367..22657)
                     /locus_tag="PK634_06205"
     CDS             complement(22367..22657)
                     /locus_tag="PK634_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOW97364.1"
                     /translation="MSRHFTRSPDLVERTIRGEHLLVPVARSEATLGSLFTLNETARF
                     VWDRAGAGQAEDGIAAALAREFGVSPAQAAADTRRVLDELLSIGALRLKGGP"
     gene            complement(22718..23863)
                     /locus_tag="PK634_06210"
     CDS             complement(22718..23863)
                     /locus_tag="PK634_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97365.1"
                     /translation="MKPRRTAILPAALAALSAAAQSVHYVRPSDTNPAITNYDNFHAA
                     FIPTGATSRTPLVLFLPGTGASPAQYEEFARRAASNGFHAIGLQYVNGDTVSTLCGGD
                     SDTNCHARIREEILFGTNASPWVEVDPGNSVAGRLEALLRYLTNQYPAEAWGAYVEED
                     GRAAWSNLIVAGHSQGASHAAFLAKKEAVRRALLFGNEADWVTGLGAAPWFSWPSPTP
                     TNRYIALTHRQDILLLQEATWTALGVAGTQVLAEASTDPACEWTHRLVTEVAPATGSG
                     WLAYHSAPIADLYTPRSNGVPVLLPVWTWMLNGPASWPALNIQWSNSGPVVSFAAERE
                     ARYRLQVAADLTAGWSNSAALVTNIDGWVDWVPPGGAPASFFRLGLE"
     gene            complement(23867..24550)
                     /locus_tag="PK634_06215"
     CDS             complement(23867..24550)
                     /locus_tag="PK634_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW97366.1"
                     /translation="MNPVRLVLLDEQRLFRELLATTLDKARDLSVVGEAGGSCRAVAV
                     CRETRPDVAIMDLRLPHINETELATCLRKHLPALRIVALTDLTDAYTIRRAVDAGLQG
                     YVEKGQPLTVLLQAVRHVAAGETYFTEIYQQRVRETAEEPFSFPKVLSAREQQILALV
                     AEGRTSRAVAQQLGLSSRTVESHRRNIMKKMDLKDMAGLVRFAVLQGFRFEECRLHDN
                     GQCAGCAGS"
     gene            24738..>25328
                     /locus_tag="PK634_06220"
     CDS             24738..>25328
                     /locus_tag="PK634_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97367.1"
                     /translation="MKKMMQRIVKPLSLAGLLWASAGSALAGPAPVQTYFIPFDEFQL
                     WRTMTNVSDTTVGTNLINVVSIAPGSTNTIIYYDHFEDGYESDIANPTQSTTRVWGDN
                     NTTNGIPPGFASDLINAGSILVLRSTVNVPHVTSPIIYDGEDKIGATKAVAVTYVAWP
                     IDPGPVLADGVDVYDTSRYGTNFYAPVGQNVSSNNHL"
BASE COUNT         3804 a         8635 c         9029 g         3860 t
ORIGIN      
        1 ccgtgctggt cctgaccagc gagaccaacc aggtcggcgt gggcgtcgtg gcgacggacg
       61 ggggcgacct tgtgacgtac atcgagcacg agtccccgag catcaccccg gaagagcggg
      121 cggcccgtat cgcggccaag gttcgggacc acaacggaaa aaagcaggcc gcgaaggacg
      181 gtctgaacgg ccagttgcag caacgccttg agaacctgga gcgcctcctg ggcgtgcgtg
      241 agtaagccat gatctccaaa accatagccg tttccgccgg aagcctggct gccctggcca
      301 gcgccgctcc ggcctccccg gaggctttgg aggcggtgag caaatggccg gtcaccgtgg
      361 tcctgggcgc cgtctgctgc ttctgcgtgt acctgatgta ccggcagggc agcgattacc
      421 gagcgagcct cgacaagatc gccggggcgc tcacgaagct ttctgagaac ctggcccaac
      481 ggccctgcat ccgggacccc cacaacgact gatccatgaa caccaacgcc attctgctca
      541 aggtgcccga tccggagggc cggttcgatg gggctttccg cttcgagtac gtgaacggca
      601 gggattgggc ggtggccggg gatatctcct atcgcaccca agctgggccg gtctcgacgg
      661 tccgggcggg attcacgacg gactttgcca cgatccctcc gttcctctgg ccctttatgc
      721 cgcctgcggg cgatggtcgg aacttctacg gcctggcggc gctttggcat gactggctct
      781 actggcatgg ggccgtcgag gggcggccaa tcacccgcgc ttacgctgat agcatcttcc
      841 tcgaactcat gctctacctc ggcgtctcgc gcccgctcgc ccgcctcatg tgggccgctg
      901 tccgctcttt tggttggtac ctgtggagaa aataccggag gcacaatgaa aatcgcatcg
      961 atcctgttgg tcgccctgct gctctgtaac ggctgcgcca cggcgctgac gaaccaactc
     1021 ctgcagcgtc aaagcgcggt ccgcgtggaa gtggacggca agaccatcat ggccggggtg
     1081 gatctcttcg ccctggacga agttgtcgag catcccttca tccacggctt cgccgcggtc
     1141 attgatctcg gggccgggta cctagcctat gacgccctgg aggatagcgg cgggagcagt
     1201 tccacccaca gcactacggc ctacacgatc cagaccggcg acaattcgcc cgtgtacttc
     1261 atggataact cgacggtgaa caacaaccag agcagcacgc cggccccggc cccggccgag
     1321 gatagcaccg aggaaacggc ccaacgcgct cggagggccc ggtagtgaag ttgcttgccg
     1381 tcattcttgc agcgctgatg ctggccggct gcggcacgcc gaagggctac gactgggaca
     1441 ccacgccgga ccacgatggc gaggagtacg tcggaggggc cccgtgaagc tgctgtgcgt
     1501 ggacagtcag ggcgagcgaa tccatgcccc gtaccgggat ctcctgcccg acatcgagtt
     1561 gcgcgggacg cccacgcccg agaagtggcg ggatacgatc tgccacgagc atggctcgct
     1621 ttgcggctgg ctcctggcgt tgcccgccca ggcggcgatc ctggccgggg aactcatcga
     1681 gaaggtcgag ctggtcttct gccgcatctt cgaccagaac gcccggttca tccccggctc
     1741 ggaggagcgc atccttgagg tgatccgcga ggagcgaccg gacgtggtct cgcggtcctg
     1801 gggccagtgg gatttggacg atacgctcgg tgagttctac gcacaggttg aatgggccga
     1861 ctgggcggag cgctacgccg cgctccaggc cgagatcggg ttcctcgacg tcggcgccgc
     1921 gggcaacgac gatgacaacg acaaggacga tgatgttgtc tacccgcagc ggctcctgaa
     1981 tagctgctgc atcatcggcg ccgcgcaccg gtccgggaag cccgcgccct ggtcgggcga
     2041 cggggcaggg gtgctctgcg tcatgtgggg cgaccgggtt tactcgccgg acgccactgg
     2101 ccgctggcgc ctgtggtcag ggaccagcgc cgccacgccg aagtttgccg gtgccgccgc
     2161 gcttcgccgg ttgcagacat ccgcgctcaa gctggcggtc atgcacgagg ccacgcgccc
     2221 gaccaaggcg gtctacgcga acatgacgca tcacgcgaag tggggcttcg gcaactggga
     2281 agaagcctgg cagaagtacg cacgcctgat gccccaggat ttgatgccgc ctccggcgcc
     2341 caaagggctt cgaagtctca ttgctccgat tgaattcttt gacttcagcc gtccctgctg
     2401 aagtcatgac gattagcggc gttttttgtg ggggcaatag tgcgtcactt gaccacgatt
     2461 tcgcaccgtt ctcaaccttg ctttattttg tccggcgatg gtatttttcg tcctttgttt
     2521 gcagggctga aatgcgcctt tcatctcggg aaaatgaaaa acagcgaaaa acgctatttt
     2581 ttcattttcc gggcctccgg agctgaaggt tccgcgttca actcgcggca ggcgcaccac
     2641 cttttttccc tgcattcgcg ccgatccgct tgatcggcgg gcagtcttct cccgcggacg
     2701 gggggatgcg aggcgcggag ccgggtcagg cggccgtggt cttcatgatc tgcgtcggcg
     2761 tcggggccgg ccagccggcc tccttgcaga cgcggatgat cgcctcgttg gtgtcgaagt
     2821 agacctgcca gtagtggtcg gtgtggcaat agggccggac cgagatcacc gcgcccagca
     2881 ggttgatgtc gagcaggttg acctcgggcg ccgggtcctt ggcgacgttc ggaatctggg
     2941 cgaccgccgc cttgaaccgg gcgatggcat cgagcgggtc cacgctgccc gccagctgcg
     3001 ccgtgcgctc gacgcgccgc accggccgga ttgagaagtt ctggatcgtg tcgctgaaca
     3061 ccttgctgtt gccgaccagg gtgctgacgt tgtcgggcgt gatgagggtg gtggtgaaca
     3121 ggcccagctc gtgcacggtg ccgacgaggc cgccgatctg cacgaaatcg cccactttga
     3181 acggccgcag cacgagcatg aaggcgccgg cggcgaagtt gccgagcatg ccgctccagg
     3241 ccgccccgac cgccacgcct gcgccggcca gcatcgccgc gaacgacgtc gtctggatcc
     3301 cgaagtagcc gaggatgccc agggccagcg cgacgttcag cgcgatggcg atgatcgagc
     3361 ccaggtactt cgtcagcgtc ggatcgatct tgctccggtt catccccgcc cggctcaacg
     3421 ccacgatgtg gccgatcagc caccggccga tcacccagaa ggcaatggcc gccaggatct
     3481 tcaccgcgag ctgggtgagg gtggtgctga cgaacgtttg aatcgcttgc atgtccatga
     3541 cgccgattct ccttgtatga cgcctgcgcg caggcggtta tgctgaccgg atgctgccct
     3601 cttcgcgcgc aacctgcccc aacagcccgg ctgatgcaag aacaaacaag ccgccgtccc
     3661 tccccctcat ccgtccctcc tcttgcttct ccgcgccctc cgcggttctc gtccttccgt
     3721 caggcgctcc ggtccggcgc gaaggagtcg agcgcgcggt tgagccatgc gatgtagtcg
     3781 tgcatgacct cgaaaacgcc gacggcgtac tccacgaaat ccttcttccg gagccgggca
     3841 tccttcaggg ggagagcgac ctcgaagctg gtgagctgga gcaggtccag gtccgggtga
     3901 tcccggggga tgtcgcgcgg cgcggtcttc agccggcgcc ccggcagcgt gtccccgaac
     3961 agcttgacga agttcggccg cgagacgatc cggcgcaggg ggcggccatc gcgcaggatc
     4021 gcctgccgga cggcccgcag gggcagggcc ggcggcatgt agagcccgcc cgccagcatc
     4081 gagtcgccgg gctcgacgtg gaggtagtag cccgcgcggt ccaccttccg cccgcggtcg
     4141 gtgatgaacg cgccgaagtg cgccttgtac ggcgacttgt cccgcgaaaa ccggacgtcg
     4201 cggttgatcc ggaaaaggca gtcctccgga tcctgctcga cgatcggcgg atcgaaccgc
     4261 gccatgcccg cgatcacgtg atcgatcgtt tccagcgccg cggctcgcgc cgcggcatac
     4321 tcggccttgt gctggacgaa ccagcggcgg ttgttgttgg ccttgagctg gttcaggaaa
     4381 tggaagacat ccgggttgat catgggggga gtgtaccacg gcccctgatg ttttccagcc
     4441 ggcgggccgg gcgcaatcag tttgtcccta gagggatttc tcggaaccgg tccggcccgt
     4501 agtgcttgtt cagctcgtcc acgatctcgg tcgagtggcc gagggcgacg tcccgtcgcg
     4561 tgccggtcac cgcgtcctcg atcgtgcgcc agccccggat cgtctcgtcg aaggcgtcct
     4621 gtggagcttg gcgattgtcg cccgcccctc cgcctccctc gccgcggcgg atgatgtcgc
     4681 cgatcgagcg gacggtctcc gcggccgagc tcaagcgccg gccgacctcc ccgctgctga
     4741 tctgcgaggt ctgaaggatc ttcagcatgg ccgggaagtc ctgcgctgcg agttcctcgg
     4801 gcgtggtgac cagcgtcatg gtgaagaccc agtccatcgc gccgtagccg gcgggcggcg
     4861 agcacatgac gcaggccagc gcctggtgcc ggtgtccctg gaagtcgagc gcgaggtgga
     4921 cgagggccgc gggatagccc tggaagtcca tccgctcgct gccgagaacg tccgcgacgg
     4981 tgaagcccgg gctgccggcc tgcatctcgg actggctcaa ggccggcgcg aggttcacaa
     5041 aggcctggac ggggtcgggg acatagggcg ccacgaacgg gtagttgccg ccgaacatct
     5101 gggcctgctg ggccttttcg ggagtccaga cctgcaggcc gatattcagg aaggcgacct
     5161 ggccccgggg tccgacggcc cggccctggc cgcgcgacgc ccgcaccagc tgccagtcgg
     5221 cgggcaactg gaccgagccg ctcccgtcgg ggaaagcggc ctgctgcatg ggcgcggacc
     5281 ttgcgggggc cgcgccgcca cccgtttcgg cggccccgga gatcgccgcg gccagttgac
     5341 ggaatgactg cggcgccgcg gcgcggcggt catgaatcac cgtcacggtg gccccccgcg
     5401 gcccgacaca ggcccacatg aaaccggcga cgggttcgcc ctgctgcgac gcctcgaacc
     5461 atgcgccgcc catccggtcg tcctggtccg cgaagccccg gttcgcgccc ggcatccggt
     5521 cgaaggccgg ggccagttgc ttcagggtgg cctggagggc ggcgcgggcc gacgagacgc
     5581 cggcgaaccg cgcgatcagc gcccggcccg taccgatctg cgcgaccacg tccatgcgcg
     5641 ccccccccga ctgcgtgacc gcccaggcga gcgtcaacgc gagcaccgcg atgatcgcca
     5701 cggtcttgtt catctgccgg accgccggct gcagttccgc gatccgggcg ctgtttcgcc
     5761 cgaattcctc cggcgccgtg gggacggctt gctggcgccg gcggatctcg gcgatttcct
     5821 tgcgcgcgct ccagccggcc ttcagctggg ctgcgacttt ctggtagtag aagcgcggct
     5881 tcccgccggc ccgggtgtcc gtggattcgt gctcgctcat ggtgcctcct gccgcgcata
     5941 agcctgacgg gtcacttcga aatgaaatcg taatcacggg tttgggtagg gacaagtgaa
     6001 atgtcggcga cgaacgccga ggggagcgcc gcctcggcgg accaggcgtt cgcgacgccg
     6061 gtcttcttcg cccgcggcga cttcaacggc gacggcgtcg tggcgcagga cgaaatggat
     6121 tccgtgttcg ccggctactg gcgcgacaac ccgacggtga tctcgaacct gctgggcgcg
     6181 ggcagcgaga cggtgcagct ggcggtgagc aacatggcga actgggggct ggcggtcgaa
     6241 acctcgaccg acctggtgac gtggtcgacc ctactggact ccgccgtccc cgtgttccag
     6301 ttcttcgacc ccgacgccac caacggctcg cctcgatttt accggctgcg ggcgccgtga
     6361 ggcccgcgcc cgccggctcg cagcgcccac gtttctccgg gcttcccgcg cgcgttcggc
     6421 cgtgctatgc atgtcacgaa attcccttga tcctggacgc aacgtggacg cgagccgccg
     6481 catggccgaa gacaacagcc gcaggatgga gcccccgggg ccgcgggtgc atatcatcac
     6541 gctgaactgg gaaaacgagc cggtcacggc ccggctcctc gcggccctgc gcgaactgcg
     6601 ttacccgaac cgggagacgg tcgtggtgga caacggttcc gcggacggct ccgcggaccg
     6661 cctggccgcc gcgttccccg aggccgtgat cctccgcgcc gggcggaatc tcgggttcgg
     6721 cgcggggaac aacctcgcga tccgccgggc gctggaggcc ggcgccgact acgtgtggct
     6781 catcaacaac gacgcccggc ccgcgcccga tgccctcgac gcgctggcgc gcgcggcgga
     6841 ggccgcgccg cgcgcgggag cggtaggcgc ggcgatttac cacgaggacc gccccaacgc
     6901 gctccaggcg tggggcgggg gaacggtcgc gcgctggctc ggctgggccc ggctggcgac
     6961 ccggcccggc gctccggtcg agtacatcac gggcacgagc atgctgctcc ggtgcgcggc
     7021 gctgcgggag gcgggtcttt tcgacgagcg gttcttcctc tactgggagg acgcggagct
     7081 gtgcgcccgc ctgcgtcggg ccggctggga aatggccgtc gccggcgaag cgaaggtgtg
     7141 gcacgcctcg tccgcgacga cggggcgtga cgccgaactc cggatgttcc acatcacgcg
     7201 cagcctggcg ctgtacctgg gcgcgcgctc ccgggcgccg ggcgtcgagt cgctcgtcgc
     7261 cacgctgttc caagcggcgg gcaagctggt gcgcgggaac cgggcggcgg cgcgcggcgc
     7321 gtggctcggc cggcgggcgg ggctggagga actccgcgcc gcccgggcgc cggggaggtc
     7381 gtgacgacat gcccggcctg gcctcgctcg caaaatccgc gctggccctg cccggccgcg
     7441 cggcccgggg cgcggcggcc ctggcgcggc gggccctggc acccgcgccc gacccggacc
     7501 gcctgctgat cttcgacgac gccttccccc acctgctgtc cgcgttccgc gtggcggagt
     7561 acaacgaggt cctgcgggcg tttccccggg cccgcgtgtt ctcgaccggg gcctgcttcc
     7621 ggtccttcaa ggaccgccgt tcgttcgccg gcgtgcgccg ggagtacgag acgcgccacc
     7681 cggagttccg cggccgcgtg tcgaagttcc atccgcttct ggatttccgc gggcggctgg
     7741 cctacacgat tttcctgcac aacatcctgt tcttcctgcc ggtgatcgag aagcgccggc
     7801 tccccttcgt cttcacgctc tatcccggct accggctgcg ggtcccggag acgaccgccg
     7861 agctgcgccg ggtgtgcggc gcgcccgggt tccgcggcgt gatcgtcacg cagccggtga
     7921 cgcgggagta cctgctggag gaaaagctgt gcccggcgga gcggatcgag atggtctacg
     7981 ggggcgtggt gcccgccgac gtgctggcgg cggcgtccgt cccgcgccgg cggatgggcc
     8041 gcgacaagga cacgctggac atctgcttcg tcggcttcaa gtacgtggag ggcgacctgt
     8101 tcaaggggta cgacgtgttc gtcgaggcag cgcggcggat cgcccgcgcc atcccggcgg
     8161 cgcgcttcca cgtggtcggc acgttcgggc cgggcgacgc ggattcggcc gagctgggcg
     8221 gccgcatccg gttctacggg ccgcagccga cggcgttctt cccggagttc tacgcccgca
     8281 tggacctcat cctgtcgccc aacgacctgc cccgctgcat ccccggcgcc ttcaatggct
     8341 tcccgacggg ctgctgcgcg gaggcggccc tgtccggcgt ggcggtgttc tgcacggacc
     8401 cgctggggca gaacgtcggg ctgaaggacg gcgaggacat tgtcctcatc aaccggcggc
     8461 cggacgacat cgcggaggtt gtgggccgct acgcgaccgg ctacgatgcc ctgtgccggc
     8521 tgggcgaacg cggccgggcg gcgttccgcc ggatcttcga tttgcggacg cagatggacc
     8581 cgcggctgaa ggtgatcgca cgggccctgg aggaaggctg acggcatgga cacggacgcg
     8641 ccgaaaaaac cgtaccgggt gctggtgttc ccgggcagca cggggatcgc gctggagatc
     8701 cagaaggcgc ttgccggctg caaggaggtc gaactgttct cggccggcgt ggacggcccg
     8761 acccacgcgc cgtacctctt cgcgcgccat ttcacggcgc cgcgcgtgga ccagcccggg
     8821 tgggtggagg cgctcaaccg gctgatctcg gagcagcgga tcgatttcgt gttcccggcg
     8881 cacgacgacg tggtcgaggc cctggcgctt cgcgcggggg aggtccgggc gcggatcgtg
     8941 tcgtcgccgc ccgagacgtg cctggtctgc cggtcgaagc gggagacgta caagcgattg
     9001 aaggacgcgg tgccggtgcc ggtggtgtac gaccggccgg aggcggtgcc ggcctacccg
     9061 gcgtttctca agccggaccg tggcgagggc tcgcgcgagg cccaggttgt ccgccatcgc
     9121 cggcacctgg aggtcctgct cgagcaatcg cccgactacg tggtcatgga atacctgccg
     9181 gggcgggagt acacggtgga ctgcttctcc gaccgcgagg cggggctgct gttctgcggc
     9241 gggcgcgagc gcgtccggac caaggccggc atctgcatcc acagccggac cgcggagaac
     9301 ccggccttcc ggcgccacgc cctcgcgatc tccgccgtcc tcccgctgca cggcgcgtgg
     9361 ttctaccagc tcaaggagga ccgggaaggg cggctcaagc tgatggaggt cgggccgcgc
     9421 atcgcgggca cgatgtccgt gcaccgcgtg cagggcgtga actttccgct gttgagcctc
     9481 tacgagcagc tgcgcgtgcc ggtcgagatc ctccgcaacc cggtcgaggt cgaggtggac
     9541 cgcgtcctcg tcagccacta ccgcccggtc gtctcgtacg acacggtcta cgtggatctc
     9601 gacgacacgc tgatcctgcg cgggcgggtc aacctgcccg tggtccgctt cctgtaccag
     9661 tgcgtcaacg agggcaagcg cctggtcctg ctgacgcggc acgcgggcga cccggaggcg
     9721 acgctgcgcc ggcgccggct cgcggggctt ttcgacgaga tcgtccggct ggaggccgcc
     9781 gagagcaagg ccgacttcgt ccgggagccg tccgcgatct tcattgacga cagcttccgc
     9841 gaacgcaagg ccgtgggcga acgccgcgga atcccgacct tcgacaccag catgctggag
     9901 ctgctattcg acgaacgagc ctgacgccaa ggagaaacgc catgtccacc cccaagttga
     9961 gcgcctacta cgtgtcggac gaggagacgc agggatacgc cgagatcctc cgcgcgctgc
    10021 gcgactgccc gatccccgac gacgagcggc tcaagcatct cccgctcttc ctgaaccgct
    10081 ccctgctcgg gcatattctg ttcatggacg agctgtaccg gctgatcctg ccggtgcacg
    10141 ggctgatcat ggaattcggc gtgcgctggg gccgcaacct agcgctgttc accatgctcc
    10201 gcagcctgta cgagccgcac aacttctcgc gccgggtcgt cggcttcgac acgttcggcg
    10261 ggttcccgtc cgtctcgccg aaggacggct cgtcgaagtc catcaaggtc ggcacactcg
    10321 ctgtcacgcc gggctacgag gagttcctcg ccggcctgct gggcagccac gagaagctcg
    10381 cgccgcgcag ccacctgcgg aaattcgagc tggtcaaggg cgacgcgacg gagacgctgc
    10441 cgcgctacct caaggaccac cccgagacga tcgtcgcgct ggcgtacatc gacttcgacc
    10501 tctacgagcc gacgaagaaa tgcctggagc tgatccgcga ccgcctgacc aagggcagcg
    10561 tgctcgcctt cgacgagctc gcgctgcccg agtttcccgg cgagaccgcg gcgttccagg
    10621 agatcatcgg gtgccgccac gcgcggctgc gccgcagccc gctggtgccg tatcagtcct
    10681 acctggtcgt cgagtagccg tggccgggcg cgatccagcg cgggggttcg tgtcgcgggc
    10741 cgcggcggcc ctgcgctggg cgggggaggg ggcggccgcg ctggcccggc ggccgttcga
    10801 gccgccgccg ccggccgacc ggctggcgat cttcgacgac atttttccct gccccttctc
    10861 ctcgttccgc ctcgccgagt tcaccgcctg cctcgcggcg ttcccgtcgg cgcaggtatt
    10921 ctcgaccggc acggccctgc gcatgctggg cgggaccgct tcgttccggg acgtcctggc
    10981 cgcgtacgcg cgcgactacc cggcgtacgc gccgcgggtc gcgaaatatc acgcgctgaa
    11041 aaacctgcgg ggccgcgcgg cctacgcggt gttcctcaac aacgcgcagg cgttcctcgc
    11101 ggccatcgag cggaaccggc tacccttcgc gttcacgctc tacccgggcg gcgggttcgc
    11161 gctgaagcag gcggtgtcgg acgagaagct gcgcagggtc tgctcctcgc cgtttttccg
    11221 gaaggtcatc gccacgcagc cggcgaccct cgactacctg gtcggcggcg ggttctgcag
    11281 gccggagcag gtggagttga tcttcggaat cgtgctcccg ctcaacctgc tcgcccgcgc
    11341 cgcgccgccg cggaagcgca agggccggga caaggacacg ttcgacgtct gcttcgtcgc
    11401 gttcaagtac atgccgggcg gcgtggacaa gggctacgac ctgttcgtcg aggcggcgcg
    11461 gatcctcgcg gcgcggcgcc cggacatccg gttccacatc gtcggcccgt acgacgcggg
    11521 cgatgcggat gtggccgcgc tcggcgaccg catccggttc tacgggccgc agccgaccgg
    11581 attcttcccc gcgttccacg ccggcatgga cctgctgctc gcgcccggcc cggcgttcgc
    11641 gcggtcgccg ggcgagttcg acggtttccc gctgggcgcg tgcatcgagg cggcgctctg
    11701 cggcgtggcc ctgttcagca ccgatccgct cgggcagaac cgctatttca aggacggcga
    11761 ggaactggtc gtcatcccgc gcgactcgga cggcatcgtg gacctcgtgg accgctatta
    11821 ccgcgactac gaccggctgt gcgaactggg ccgcgccggg caggccgctt gccgccggtt
    11881 gtacgggtgg gaggcgcaaa tggcgccgcg gctgcggatc ctcgcggagt tgctcggcgt
    11941 cgagccgccg cggctggacg cggaagcgag agcggagctg ccgtgacgcc gaagccgaac
    12001 atcgtgctcg tcgggattgc cggcacgccg gacgacttcc tgctgtcgcc ctacgccctg
    12061 aaggcctggc tggccgcgaa gcccgcgatc cgcgacggcg cggagatcga gatcctgtcc
    12121 tatcccttcg tccccgcggg ccagcgggcg gaggcggcgg agcggatcct gcgcgacgta
    12181 tgcgcgcgcg aaccgcgcct ggccgggttc gcctgctatc tttggaaccg ggagactgtc
    12241 gacctgctcg cggccggcct caaggcgcgg cgcccggaca cgctcgtcgc gctcggcggg
    12301 cccgagatat gcgaggagga gatccgcgcg gggcggttcg acggcgcgtc cgcggatttc
    12361 ctcgtcttcg gcgagggcga gaaaccgttg gaggcgctgg cgctccacct gctcggcgcg
    12421 cggcccggac tcgacggcgt gcgcggcctg gcgcggcggg aggaggggcg gtgggtgacg
    12481 cacggggccg atgccatcga gcggctcgac caggcgccgt ccccgtacct gaccggcgcg
    12541 atcccggacg aactcctcgc gcggccgggc atccgggcga acgtcgaaag ccagcgcggc
    12601 tgcaatttcc gctgcgccta ctgcttctac cacaaggatt tccgcggcat ccggtaccgc
    12661 gatcccggcg cggtggtgga ggaaatggcg ctagtggacc gccggggcat ccggatgtgc
    12721 cggatcacgg acgcgaactt catttccgac cgcgagcacg cgaagacgat cctccggggc
    12781 cttgtcgaac gccggttgca actgtccctg ttcgcggaga tcctgccgca gttcgtggac
    12841 gaggaattgg ccgggctgtt tggcgagttt gtccgctgcg cgcccggcaa cgtgctgatg
    12901 ctggggatcg gcctgcagac cacccacgag gagtccctgc gggcgatccg ccgggcgatc
    12961 ccgctgcgcc atttcgagcg cgcgttcgac ctgctcgccg gccagggcgc gatcctcaag
    13021 acggacctca tcctcggcct gccgcgcgag acgcgggaga gctacttccg gacgatcgaa
    13081 ttcatcgcgg agaagctgcg ccgcgggacg cacttcttga gcctcgccgt gctgcgggtc
    13141 ctgcccggct cggacatggc ggagatcgcg cggcgggagg gactcgtgcc cgacacgcgg
    13201 gatgccggcc gtttcgtgta cgagaccccg acgatgccgc ggccgcacat gctggaatgc
    13261 ctgcggctca acgccgcggc gttccgcgtg ttgagcaccg tgcacatgac gcagggcccg
    13321 cgcatccgcg acctctactt cgaggcgaag gaccgcctgg gcgtcccgca cgtgcgactg
    13381 ctgcggcggc tcgcccggga ttttttcgac tatctccagg ctcgccgcac cgacttcgtg
    13441 aagccggatt tcccgaacgc ggaggactac gcgtccaagg acatccgccg ggacattccg
    13501 gacgactggc tggcggagcg gctggcccgg ctgggcgagg ccggcctgcc ggagacggag
    13561 agctgaacgg cgatgcgcac gtgcaactac ctgcccctgg gccggatggc ccgcgcggtg
    13621 cgcggcgcgt ggcggatgct gctcgcggcg ggcggaacgc gcgaatgccc ggcgtgcggg
    13681 cggcggatcc ggagattcca gcctttcccg aaggaggcgg ccgacctgct cgcccggcac
    13741 cggttccggc tgccgtggaa atcgtccgcc tgggaaacct tcaaccggga ggagtacgcc
    13801 tgcccgttct gcgacgcgac ggaccgcgag cggctctacg cgctgtacct gcgggagcgg
    13861 ctggcgccgg gcttggggga cgggttgcgc ctgctggaca tcgggccgtc gcggccgatg
    13921 acccgtttcg tccgccgccg gctgcgcgcc gcgtaccgct cggcggacct gtgcaatccg
    13981 gaggcggacg accgggtgga tcttgaagac atgcggatct accgggacgg cgagttcgac
    14041 gtgctgctct gttcccacgt ccttgagcat gtccccgatg accggcgtgc gatgaccgag
    14101 ctgttccgcg tcctgcggcc gggcagctgg gggatcgtca tgacgcccat cccgctgggt
    14161 gtcgaaaaga ccgacgagga cccgggcgtg accgacgtgg cggagcggct ccgccggttc
    14221 gggcaggagg atcacatccg gatttacgcc ggcgcggact tcacggagcg gttgcaaaag
    14281 gtcgggttcc gcgttacaaa gctgggccgc gggcacttcg ggccggaggc cttcgagcgg
    14341 tgcggcctga cgccgcagag cgcgctgtac gtggtcgaca aaccgatggg agcgggagca
    14401 tgacggccga cctgggacac atcctcgagt actgggaccg cgaagacgtc gagtccatgt
    14461 atgacaagca cctgctcaac gccgagatcg agttgatccg ccggcgtctc gctccgggct
    14521 tgaagatcct cgacgcgggc tgcggggagg gcgagggcac gctggcctac tcgatgctgc
    14581 cgggcgcaaa ggtccacgcg gtggacttct ccgcgacccg cctgcgcaag gcggcggctt
    14641 gcgtggccgg ccgggacaac gtcaccttcc agcaggtgga cttcctcaag ccgtaccggc
    14701 tcgaccgcga cttcgacgcc atcgtctccc agcggttcct catcaacctg cccgcgtggg
    14761 aactgcaggg ccgggtcctg ctggagctca tggatcacct caagcccggc gggcgcctgc
    14821 tgatactcga gggcagccag ccgggcgtcg acgagctcaa cggcttccgc gccgcgtgcg
    14881 gcctggagcc gatcccggtc aagtggcaca acctgttctt cgacgacgag gtcctggtcg
    14941 cgttcatgcg ggagcgcggt catccgctgg cggaggtgga cgggttgggg gcctacttcc
    15001 tgctgacccg cggcgtgcgc ccgctgctgg acgcgaagct cgactggaac tgcgagttca
    15061 accgccgcgc cgccgcgccg gcggtcgagt ctgcccttcg gctcggggcg cgcttctcgc
    15121 ggctcaagct ctgggtgttc cggaagtgaa gccgtccctc cagccgctgg cgatcctcgg
    15181 cggccggccc gcctttgccg agcgcctgta cgtagggcgt ccgaacatcg gggaccgcgg
    15241 gcgtttcctg gcgcgcgtga acgatatcct ggaccggcgc tggctgacca acaacgggca
    15301 ctacgtccac gaattcgaga aggccctggc cggcctgacg ggcgcgcggc atttcatcgc
    15361 cgcctgcaac gggaccgtcg cgctcgaact ggcggtccgc gcgctcggcc tgcgcggcga
    15421 ggtgatcctc ccttcgttca cgttcgtggc caccgcgcac gccctccagt ggcaggagat
    15481 cacgccggtg ttctgcgaca tcgagccggg ttcccacacg ctggacccgg cccgggtcga
    15541 ggagcggatc acgccccgga ccagcgggat catcggcgtg cacctgtggg gcacgccgtg
    15601 cgccgtcgag gacctcgcgg ccatcgcccg ccgccgcgac ctccgcctgg tcttcgacgc
    15661 cgcgcacgcc ctcggcagca cccggggggg gcggccggtc ggcggcttcg gcgacgccga
    15721 ggtcttcagc ttccacgcga cgaagttcct caacaccttc gagggcggcg ggatcgcgac
    15781 caacgacgac gagctggccg cccgcctgcg cctgatgaag aacttcgggt tcgcgggcta
    15841 cgacgaggtc gtgtacctcg gcaccaacgg caagatgagc gaggtgcagg ccgcgatggg
    15901 cctgactggc gtcgagagcc tcgacgagtt catcgcctgc aaccggcgga accacgagcg
    15961 gtatcgcgcc gcgctggcgg gcctggccgg cgtgcggctg ctggagccgg cgcaaggcga
    16021 gcggcacaat ttccagtatg tcgtgctcga gatcgacgag accgctgccc tggcgcgcga
    16081 cgaactgctc cgcgtgctct gggcggagaa cgtcgtcgcc cgccgctact tccatcccgg
    16141 ctgccaccgg atggagccgt accggacgct gttcccggac gtcgcggccc gcctgccggt
    16201 gaccgaacgg atctgccggc gcgtcctggt cctcccgacg gggacggcga tcggcgaggc
    16261 cgagatcgac gcgatcggct cgatcctgcg cgcggccctg gcgcgcggcg ccgaggtccg
    16321 cgccgccctc gcccggagcc ccggcccatg agcgaccccg gcgggccccg gccgcgcgtc
    16381 agcgtcgcga tgatcacctt ccagcacgag cgcttcatcg cccacgcgat cgagagcgtg
    16441 ctcatgcagg acgccggctt tccattcgag ctggtgatcg gggacgacgc ctcgaccgac
    16501 cacacgcggg aggtcgccgg ggaatacggc cgccgccacc cgggccgcgt ccgcctgctg
    16561 acggcggaat cgaaccaggg cgtggcgcgc aacttcgtga agacgctacg ggagtgccgc
    16621 gggcagtatg tagccctgct cgaaggcgac gactactgga cccggcccga taaaatgagg
    16681 aaacaggcgg atttcctcga cgcgcacccg gaacatgccg cgtgttttca cccggtcaag
    16741 gtccgggccg acggcgaggc cgggacgcgg cacgtgcagc gggtgcgccc cccgcgcggc
    16801 ggatacgggc tggaggacct gctccgcgcg aacttcatgc acacctgctc cgtcatgagc
    16861 cgaaaccatc tcattcccga ttggcccgag ccgttcctgg acctcaagat gctcgactgg
    16921 ccctaccaca tcctgaacgc cctgcgcggc acggtcggat tcctgccgga ggcgatggcg
    16981 gtgtaccggc tccacgcggc cagcgcgtgg tcggccggcc ggctccggga ccgcatcgcg
    17041 gagacgcgcc gcatgctgga acgggtcaac gcgcatctcg gcgggcgata cgaccgggtc
    17101 gtccgccgct ccctggcgga atgcgactgc caggaggcga tgcagtttgc gcgcgagggc
    17161 aaccggcggg cggcgcgccc gctggccgcg tcgtgcgtcc gcgcgtaccg gtcggggaac
    17221 ggcccgccgc tcgcggccct gctcgccctg ggcgcggtga tggcctggcc ggcgctgtac
    17281 cggctggtgc tggcgaagaa gccgctatgg agatagcggc gggcggtccg cgggccgcag
    17341 ttcggccagc acctcgatga tgtggcgggc ggtggcctcc cacgtgaatt ccgccgcgcg
    17401 ccgcgcgccg cgcacccgca ggccgtcggc cagagcgcgg tccttcagga tacgctccag
    17461 gccttcggcg atggcgccga cgtcggccgg atccaccagc agggccgcgt cgcccgcggc
    17521 ctccggcatg gcgctggcgc gggaggtcag caccgggcag ccgcaggaca tcgcctcgag
    17581 gatcggcagg ccgaatccct cgtagagcga cacgtacacg aacgcgtcgg ccgcccggta
    17641 gagcgccggc aggtcggcgg cgggcacggc gcccaggcgc cggaaccggt cccgcaggcc
    17701 cagtcgttcg acggcgcgaa acaccgcgcg gtccttccag gacttgccgg ccgtcaccac
    17761 gaaattgtgc gggacccggt cgcggatccg ggcgaaggcc tcgagcgtgc ggacggcgtt
    17821 cttgcgcggc gagaggctgc cgcaatagaa gaagtagggg cggtccaggc cgtaacgcga
    17881 ccggacgctt cgcagggcct cctcggcgag caggccggga gcgggcgggg aaaccccgag
    17941 gggcacggcc cggacgcgcg cgggatccag cgggaacagg tccagcaagt cccggcgggt
    18001 ggactcggag atggcgatga tccggtcggc gcgccgcagg ccgcgcggga agagcgcccg
    18061 gaaggcgagg acgtccagcg gcttgtactc gtcgatgctc gcgccgcgga ccgggtagta
    18121 gagcaggtcg tagacgatcg cgacggtttt cacgccgcgg ggaaggaagg cggggacgaa
    18181 gttcttggcg cagagcagga cgtccacggg ccggcggcgg agcgcgaggg gcagggcgac
    18241 attttcctgc cacagcgtgc cggggccccg gagccggtgt tcctccgctc ccgggaacgc
    18301 cccgccgtgg cgggcgctgt ggtggtagat cacgtaccgg ttggcgggat cgaggcgggg
    18361 cagcaggggt attacgtttt ccaggaacgt cgtaacgcct cccacgcgca ccgcaagccc
    18421 ccgggccagc acgccgattc tcatgccggt ttccccggcg cccgccgcag gcgccccgcg
    18481 atctcggcga cctcggccca gcgcaggctg cccgagagga acgtcacggc cgcgaagcag
    18541 gccgcccaca acgcggcggc ggcgaggccg gccggcagcc cgaggcgcga gccgaacccc
    18601 aggggccatg ccgcggcggc ggccagcgcg ggcgccgcca gccgggcgac cggcaggccc
    18661 tgcttccacc cgagccggac gtgcgtcatg accagcgcca tgaagccggc ggcgcagagg
    18721 aactgggcct gggcgacgcc ccgggcgccg cccgcgggcc cgaggacgcg gaagaggagc
    18781 gggaacgaca ggaccagcgc gaggctgccg gcggcgaacg gcgtggcgct tttcaggagc
    18841 atgcccagcc acccgtgctg gaacccgatc cagaggaata cgacggagaa cagcacgagg
    18901 ccgatcgcgg ggaccgcgcc gtcgtaggcc gcgccccaga ccagccggac cagcccgcct
    18961 ccggcggccg cgaacacgcc ggccgcggcg agcgcggcga ccgcgccgaa gcgtgtggcg
    19021 atgccctgcc agcggagggc gcggtccagg ccctcgcgtt catacaccca cgtgatcgag
    19081 ggaaacagcg cgccgccgac ggagaccagc gtcatccgga cgatatcccc gacacggacg
    19141 gccagtccca tgaaggcgat ctgggcggcg tccatgccgg cgccggccat caggtagggc
    19201 ggcaggttcc cgtagacgct gaccagggcg ttggagaatc ccgcggtggc gacgaagcgg
    19261 ccgtactcgg gccagcgggc gcggggcggc cagccggccg cccattgcat gggcccgcgg
    19321 gccagggcga acgccgccag cgcgtacagg gcgtcgccca gcacgcaggc gcggggcacc
    19381 catcggaatc cggcggccgc gtaggcggcc agcaccacgg ccgggaccag cgacgcgttg
    19441 agcaggttca gcgcggcgac ccgcggcatg cggcgctcgc tgtacaggac ggcatggaag
    19501 ctcatgccga tgaccagcag caggacgctg gcgcccgaga ggacggccgc ctcgcccccg
    19561 agcgcgacgc gcgtggacgc ggccagcagg aggccgctgg ccagcgcgcc gaggccggcc
    19621 agggccagcc ggaacgcgaa cacgagcttg aagagccgtc ccgcgtcggc gtcgcggccc
    19681 tccgcccgca gcgcgacgta gtagcggccc atcagttcgc tcgagcccga gacgcacagg
    19741 atcatggcca ggctgatcac gctgcggaaa aggacccacc ggccgaaatc ctccgcgccg
    19801 aagaggcgcg gcatcagcat ggtcgtgcac agcgagacca ggatgatggc ggcctgcccc
    19861 gcgccgatcg ccatggcgcc ggacacggcg cggaacgatt cctctcgagt cggttcgtcc
    19921 accggatgct ccaggccggg catcatagcg gagggggcgg cgcattccaa tcccgtcgag
    19981 gcgggcggtg gtcaggcgtc ccggaactcg gcccggcaca attccacggc gcgggcgtcg
    20041 ggagtgaagt ccagccggta ggagggcgtc cgggcggtca cagccgtcca ggcatcgagg
    20101 agctgttcca ggccgtgttc cgagtagaac ggcgcgacgg tggtcgccag gagggccgtg
    20161 agcggctcgt ttccctcgag ccggcgcagg cggttttccc gcgcctggtt caggtggacg
    20221 atggcacgga gggggacggt ggcgctgttg acctgcggat cctcgccgtg ccagggggag
    20281 gattcgagca ggacgcccgc ggccgagttg cgcaggatga cccggtcgtc gttcaggagg
    20341 gtggcgccga ggccgcgcca gaggcgggcg agggtggtct tccccgcgcc ggagcgcccg
    20401 gcgaagatca ggccccggcc ccggtcctcg atgccgcagg cgtgcacgac gcccgcgccg
    20461 aagtggagca ggcggttcag gaggatcgtc tgatcgtacg ggtggttgag ggcgtactcg
    20521 gagggcgcgg tcatgcggcc gtagagcggg cggatgtcgc cccgcgaaaa atcgggttgc
    20581 gcgttgacca cgggaatcca ctgcccgtcg gccacgcgct ggagttcgat gccgagccgg
    20641 ccatcgccgg tgcgcccgag gcgccaggtt tcgctttgcc agaggagcgc gcccgtctcc
    20701 acgggagcga gcgaggcgtc ccgggggtcc aggacgcgat actcggcctg cgcggggccg
    20761 gagggcggcc cggcgcggaa cttcaggtag gcttccggca gccgcgggac caggtcatcc
    20821 cgggcgccgc gcaactggaa ccggactccg gcgagggtga acgccagcgt gttcaagacg
    20881 cgggatcagg agccgtagtc gaagccctgg ccgccgaact cgtcctggca gggcacgtcg
    20941 tccgggccgg tcttgcaggc ggccatggag accacgcgct tgtcttcgag cttgatccgc
    21001 ttcacggcgg gcttggtgta cttcttcggc gcctgttcgt tttgcttgtc catagggcct
    21061 gctcctcttc ttcgattcgc tcgcgatcgt ccgaactatc gcacagatcg gtcgggatgc
    21121 aaccccggat ttccgcttcg ccggccgcgc gccctcgccg gccaaggtag tgctgtcctt
    21181 tattccatat atagccatat ggctatatat gaatatatac taggctggct caacggcacg
    21241 ggccgcgtgg ggggcgaagg gccgcggaag cggcggcccg gtagacggtc ctatttgccg
    21301 gcggagggcc gccgggtcgc ccgcgcgcgg cagacagcgc aatagtaggc cgccgggagc
    21361 ccgggggcgg aggcttcgtg ggcggatgtg gcggcgcagt tgaggcacat cgtgcggtcc
    21421 aggcacgagg cgcattcgct gtccgcgggt gcccggcgcc gcaggagctc cgccatatgc
    21481 cggcgccatc cggcgacgga cccgtccagc aggtcgtagg gatcccggcg ccacatgagg
    21541 cagggatgca gcatcccgcg cggatccacg tggaaggtca cccggcccgc cccgcagcgg
    21601 aagagccgcg ggtcggggcc ccagccctcc gtctttcccc gccactccgc gggactccgg
    21661 ggctcggcgt cggcctcgac ctgccaccgc gcggcctcct cgggcggcag ccgctcggcg
    21721 aacggttccg tgcccccgtc gagccgcgcg ttgacgatcg catcgtaccg gaacggccgg
    21781 ccgagcgatt cggcccaggc cttcatcgcg gcgaactcgt ggacgttgct gcgcaggacc
    21841 atgctcttga ggtacacggg aaggtcccgc gcgaccagcc ggtcaaggcc gcgccggaac
    21901 gccgcgaacg agcccggccc gccggtgatg cgctcgtacg tctccgcggt gtgcccgtag
    21961 atgctgatct cgatccggcg gggcggctgc gccgcgagga agtcggcgag cccgtcatcc
    22021 acgcgcgtgg cgttggtgaa gagcgtcatc aggaagccgc gccgccgggc atggaggtag
    22081 atctcccgga agtcgcgccg caggaagatc tctccgccgg tgacgaggag ggagagcacg
    22141 ccggcgtccg ccagcgcgtc gagcaccttc cgcgcgcgcg cggtgtccat ctcgccgtcg
    22201 gcggcgccgg gggcgtggat gtagcagtgc cggcagcgca ggttgcagcg cagcgtcagc
    22261 tcgatgctgc cggacatcgg cagcgtatcc tcgccggccg ggcgtcgggc tagttcgtcc
    22321 aggaagtccg cggagccgat ctcggggatc gtgcagtcgg agcagctcat gggccgccct
    22381 tcagccggag tgcgccgatg gacaacagtt cgtcgagcac gcgccgggtg tcggccgcgg
    22441 cctgggcggg cgagacgccg aattcccggg cgagggcggc ggcgatcccg tcctccgcct
    22501 ggccggcgcc ggcgcggtcc cagacgaacc gggccgtttc gttgagggtg aagaggctgc
    22561 cgagcgtcgc ctcggagcgg gcgacgggca cgaggagatg ctcgccgcgg atggtgcgct
    22621 ccacgaggtc ggggctgcgg gtaaagtgac ggctcatgtc gatccctttc tgtccggaag
    22681 tataccgcgc gcggcgtgcg ttcgccaatc ggcgccctca ttccagcccc agccggaaga
    22741 acgaggcggg ggcgccgccc ggcgggaccc aatccaccca tccatcgata ttggtcacca
    22801 gcgccgcgct gttgctccac ccggccgtca ggtccgccgc gacttgaagc cggtagcggg
    22861 cttcgcgctc cgccgcgaac gagacaacgg gcccgctgtt cgaccattgg atgttcaggg
    22921 cgggccagct cgccggcccg ttgagcatcc aggtccagac ggggaggagg accgggacgc
    22981 cgttggagcg cggggtatac agatccgcga tgggcgcgga atggtacgcc agccagccgg
    23041 acccggtggc cggggccacc tcggtcacca ggcggtgtgt ccactcgcag gcgggatcgg
    23101 tggacgcctc ggcaaggacc tgggtgcccg ccacgcccag cgcggtccac gtggcctcct
    23161 ggaggagcag gatgtcctgg cggtgggtca gggcgatgta ccggttggtc ggggtgggcg
    23221 atggccacga aaaccaaggc gcggcgccca ggcccgtcac ccagtcggcc tcgttgccga
    23281 acagcagggc gcggcggacc gcctcttttt ttgccaggaa cgccgcgtgg ctcgcgccct
    23341 gggagtggcc ggccacgatc aggttcgacc acgccgcgcg accgtcctcc tccacgtagg
    23401 cgccccaggc ttcggccggg tactggttgg tgaggtagcg gagcagcgcc tcgaggcggc
    23461 cggccacgct gttgccggga tccacctcca cccagggcga ggcattcgtt ccgaagagga
    23521 tctcctcgcg gatccgggcg tggcagttcg tgtcggaatc gccgccgcac agggtggaaa
    23581 cggtgtcccc gttcacgtac tgcaggccga tcgcatggaa gccgttggag gccgcgcgcc
    23641 gcgcgaactc ctcgtattgc gcgggcgagg ctccggtgcc gggcaggaac aggaccagcg
    23701 gggttcgcga tgtcgcgccg gtcgggatga aggccgcgtg gaagttgtcg taattcgtga
    23761 tggccgggtt cgtatcgctg gggcgcacgt agtgaacgga ctgggcggcg gccgacaggg
    23821 cggccagcgc cgccggcaga atggccgtgc gccgcggctt cacgcttcag ctcccggcgc
    23881 agccggcgca ctgaccgtta tcgtgcagcc ggcactcctc gaacctgaag ccctgcagga
    23941 cggcgaaccg gaccaacccg gccatgtcct tgaggtccat tttcttcatg atgttccgcc
    24001 ggtgcgattc cacggtgcgc gagctcaacc cgagctgctg cgcgaccgcg cggctggtcc
    24061 ggccttcggc gacgagcgcg aggatctgct gctcgcgggc gcttaggacc ttcgggaacg
    24121 agaagggctc ctcggccgtt tcgcgcacgc gctgctggta tatttcggtg aaataggttt
    24181 cgccggcggc gacatgccgg acggcctgga ggaggaccgt cagcggttgg cccttttcca
    24241 cgtaaccctg cagtcccgcg tccacggcgc ggcggatggt gtaggcgtcg gtcaggtccg
    24301 tcagcgcgac gatacggagc gccgggaggt gtttccggag gcaggtcgcg agttccgtct
    24361 cgttgatgtg cgggagccga aggtccatga tcgcgacgtc gggccgggtt tcgcggcata
    24421 cggcgaccgc ccggcaggag ccgcccgcct cgcccaccac cgagagatcc cgggccttgt
    24481 ccagcgtcgt cgccagcaat tcccggaaca accgctgctc gtcgagcagc acaaggcgca
    24541 ccggattcat gggaaaaccc tactcgcgaa ggcctgattt gcaagcgttc cgtagaaata
    24601 cgcagaagcc gccgattgaa ttaccaccac tccgggcgag attagctccc tgggaaaaaa
    24661 agcgtcgggg tcaatttgag aaccgtatcc acagaaccaa gcgaccggcc agatcggccg
    24721 ggagcaggga ggtccgcatg aaaaagatga tgcagcgaat cgtgaaaccg ttgagcctgg
    24781 ccggccttct gtgggcaagc gccgggtccg cgctcgccgg gcccgcgccg gtgcagacct
    24841 atttcattcc gttcgacgag tttcaactct ggcgcacgat gacgaacgtc agcgacacga
    24901 cggtcgggac gaacctcatc aacgtcgtct ccatcgcgcc gggctcgacc aacacgatca
    24961 tctactacga ccacttcgag gacggctacg aaagcgacat cgccaatccc acgcaatcca
    25021 ccacccgcgt gtggggcgac aacaacacca ccaacggcat accgcccggc ttcgcgagcg
    25081 acctcatcaa cgcgggcagc atcctggtgc tgcgcagcac ggtgaacgtg ccccacgtca
    25141 cgtcgccgat catctacgac ggcgaggaca agatcggggc gaccaaggcc gtggcggtca
    25201 cgtacgtggc ctggccgatc gaccccgggc cggtgctggc ggacggcgtg gacgtgtacg
    25261 acacatcgcg ctacggcacg aacttctacg cgccggtcgg ccagaacgtc agctcgaaca
    25321 accacctc
//
LOCUS       DAOOTK010000037        23490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1507_length_23490_cov_5.060252, whole genome shotgun sequence.
ACCESSION   DAOOTK010000037 DAOOTK010000000
VERSION     DAOOTK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23490
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_23490_cov_5.060252"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PK634_06225"
     CDS             <1..1258
                     /locus_tag="PK634_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOW97368.1"
                     /translation="EALATLSRGDVDLVLLDVLMPQRDGISLLREIIDSFPHLAVVMV
                     SGSTSVRPVVESIQAGALDYLPKPFEVDDARRMVARALERAALKRNVEILQREVLREF
                     PVQDIVGHAPAFLKAIENVRKAAETSATVLIEGESGTGKELMARLLHFQSNRREEPFV
                     AVHCASLPETLMESELFGHEKGAFTNADRRRLGRFDVAGAGTIFFDEIGEMTLATQVK
                     LLRVLQEHEYMRVGGTQTIKTRARVVAATSRDLRAEVEAHRFRDDLFYRLNVVPVRLP
                     ALRDRREDIPRLVEHYFTAFRAALNARARGFSADARSLIGQYDWPGNVRELRNLVERV
                     LVLHGQQEEIGPECLPDEIRLRERGAGVAPLSLEEAVNDFERRMVVKALAEAGGVQTR
                     AAERLGTTRRILRYRMEKLNIPRNGD"
     gene            1331..1747
                     /locus_tag="PK634_06230"
     CDS             1331..1747
                     /locus_tag="PK634_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOW97369.1"
                     /translation="MKFYRKELWFNTPARRAFVNITPQVEECLRESGIREGLLLCNAM
                     HITASVFINDNESGLHADFEKWLEKLAPEKPYTQYAHNGAEDNADAHLKRTVMGREVV
                     VAVSGGKLDFGPWECIFYGEFDGRRRKRVLVKIIGE"
     gene            1758..2936
                     /locus_tag="PK634_06235"
     CDS             1758..2936
                     /locus_tag="PK634_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOW97370.1"
                     /translation="MRKTWIALLLISALSAGAAESWVSFVITANDLPENGWVHITGNH
                     EKLGSWNPSAVPLQKRADGFWVLKVPVETGSTLEYKFTLGSWDTEALAADGSVPPNHV
                     LRVGGDTTVREVVTRWPTPRDPAAPKYTGTVEYHAGMAGEGILPRDVLVWLPPSYATD
                     PERRYPVLYMHDGQQVFDPATSTHGADWGVDETATRLIAEGRLQEIIVVAATCTSNRF
                     PEYSETEAGSAFRRFLARDLKAFIDAKYRTMPDRAHTAVMGSSMGGRVSFLLAWEYPD
                     VYSMAGCLSPSFSDETLALARAKQEDPPAVRFYMDNGGIGLEERLQTGIDQMLPLLQD
                     MGFEQGDNLVWFRDIAAEHNEAAWAKRVWMPLLYFFGQGPQDWIRELPPITGGEPSAP
                     "
     gene            2938..5166
                     /gene="priA"
                     /locus_tag="PK634_06240"
     CDS             2938..5166
                     /gene="priA"
                     /locus_tag="PK634_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOW97371.1"
                     /translation="MPRIARVVVEVARNREFDYLIPARLEGQLRVGMQVVVPFGHSEA
                     RGYVVGLSDEARHPKLKEIRDTVGQAVLVDERLLQLARWMSEYYLAPVERTIHTVLPA
                     PVRRKGTGFKERLLVQPAEAAMDDLAVERLRAKAPKQAAVLDALRAGGPAFLNDLARA
                     AKTTSAAVRALEKKGWVRIGRQAVHRDPLAGQELLRTQPLALMPQQKEALDLVCRAMD
                     TLEPPVVLLYGVTGSGKTEVYLQAIARALEKGQGAIVLVPEISLTPQTIERFHGRFGD
                     TIAVLHSSLSDGERHDEWHRVRQGRARVVIGARSALFAPVEKLGLIVVDEEHEPTYKQ
                     EETPAYHARDVAVMRGHLEGCAVLLGSATPSLESFQNARIGKYGLARMPARVDHRTMP
                     AVRVVDLREEAERAGRPNVFSGDLVDAIHSRLSRAEQTMLFLNRRGFATSLVCPQCGY
                     VARCNECSVAMTYHREPGAPEILLCHVCGERRIVPARCPNDKCRTPAFKFAGVGTQRI
                     ESIISRLFPDACVRRMDSDTTGRKDAHREILGEFRAGRIDILVGTQMIAKGLHFPNVT
                     LVGVLYADLSLHMPDFRAAERTFQLLTQVAGRAGRGQATGEVIIQTYTPFHPAVQAAR
                     RLDYEGFFDQEIEFRKELGYPPFGHLVCLHLQGPGEAKVQFAGEALVRALKPKLPASV
                     KLAGPTPAPLARARGQYRYQVLLRAPSTRSMTAPLKAVLETFKWPEDVRCIVDVDAVS
                     LL"
     gene            5427..8114
                     /locus_tag="PK634_06245"
     CDS             5427..8114
                     /locus_tag="PK634_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOW97372.1"
                     /translation="MRSGHGGGVRDGLMALLLLGCAAPLAAHVLPFEEDDTLEEIRSK
                     IQHNGYRFTVNTNRFTNMTPGERRAMLGGRPWEGPEPAPAVQFKARRAEDLPSAYNIT
                     NLNGHTYIGPIHDQGLCGCCWIFGALAAAESAFNLDRGRYDNQCVDFSEQYAMDCYSM
                     PDSPYRHINAFGCEGGSHWIAAERLVAYGVTEETNAPYEMMQLDWERHMGKPLTRFKS
                     WGRLPVNDIPAIKTAIMTYGAVAARVAASPAFTFYSSGIFEDANTNADLSSCNHVVAL
                     VGWDENGGDGYWILRNSWNVDWGEGGYMRIKYTSAHAAGGVIYIEYDPDEVSAVPVCL
                     SASDGAYTNQVRLTWWAADGAEGYRIFRDNGADTNDAIQIGITSSTNYDDTSAVAGTM
                     YSYWLKSTNGCGISGFSYFQDSGWRASPTGAIGVLPPMLSYAVAYGSSNPAAQSFIVT
                     NSGAIGFNFTNSTTYSADASGWWQAVPGTGSVTAFASLALTGTVDVAGLNAGTYYANS
                     MIASSEADNSPYSLVVALTVNKGDQIITFPKIPDQVVTNTVGISATASSGLPVVLRVV
                     SGPAVLDGTNLSFTGAGWVNVKAYQAGDTNWNEVYVYQGFRVFGLYTLTIESAHGTCS
                     PVVGIYTNVEDSVLTNTVTGADIHGDTQYVCAGWAGSGSVPPEGGTTDCTFTITSDSV
                     LTWLWTTNYWLASTGGLHGSVTVPSGWQPAGATTAITATADGYYHFTNWTGDVSGPDV
                     QANPLNLLIDAPKAVTAHFAENLTTNTGTPEWWLAQYGLTNGGVSFEQAAADDTDRDG
                     EAAWEECVAGTDPTNRVSALGIHDIELSGTLIEIEWPSVSGRLYSVFCATNLPVGEFN
                     LLGTASNLQATPDVNTYTNTTDTNVPVLFYRIGVRKGLE"
     gene            8179..8610
                     /locus_tag="PK634_06250"
     CDS             8179..8610
                     /locus_tag="PK634_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOW97373.1"
                     /translation="MSRVKIELPESLPFVTELEVRIGDVNYGRHLGNDAVLGLLHEAR
                     LRFLKQNGFTEENAGGAAMIMLDAVIVYAAQAFHGDRLRAEVGAGDFGACGCDFYYRL
                     TRIADGREIARAKTALAFFDYAAHRIARMPAEFKARLATPA"
     gene            complement(8582..9184)
                     /locus_tag="PK634_06255"
     CDS             complement(8582..9184)
                     /locus_tag="PK634_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97374.1"
                     /translation="MKKRLLVIAAAGLLMQGSVRAQQPSQADMAEFMKAMQAMTAAAS
                     NSAPVVDFRALKALLPESLDGFTRTSATGEKNTAMGMTVSQAEARYEQGDASIEINVS
                     DNGGMGGLMSFAQAAWASSEIDRETDTGFERTTAYGSHKAREEYDNEDQRGTIEILAG
                     GRFMVKATGYKVAWEALQAAVKKIDLDALAALKPASPAAP"
     gene            9293..9691
                     /locus_tag="PK634_06260"
     CDS             9293..9691
                     /locus_tag="PK634_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97375.1"
                     /translation="MRHPKAIGWEDKLRALFDRIDDRLEERYGKQYPLHSNRPPRGTT
                     SSREMDGLFDIGAAFSAGYGSAVGRGYVVEIRMATPARVPPAVMERIEQEVVSMIEAE
                     VKTEFPGRDLKVSRDGPVYKIHGDLSLGEA"
     gene            complement(9756..10490)
                     /locus_tag="PK634_06265"
     CDS             complement(9756..10490)
                     /locus_tag="PK634_06265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOW97376.1"
                     /translation="MAFDKKSIRAVIFDYGNTVVEFSRTQVVVCDAALADVLEKHFGK
                     PDFEKLSAIRDRNRLAPYAGDPPLYRENDLVEITTAMIQELYGLDPSPEQLAEILRAR
                     FEVFVRIVRAEPEVYDLLDRLAQRYRLGLLSNYPDGDAIRESLAKTGLDAYFKSVVVS
                     ADVHLVKPHPVPFLTILDDLRVLADQAVLVGDNWVADVQGGKRAGLNVVMLRQWETPE
                     KLPRKPGDFEPDVTIGKLAELDALLL"
     gene            10553..11797
                     /locus_tag="PK634_06270"
     CDS             10553..11797
                     /locus_tag="PK634_06270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW97377.1"
                     /translation="MAIRWVRRVFHAGWERNVVVTGVSQFFSIMGFAFALPFAPYYMQ
                     DQLGVTDPARLKFWVALFGAATPLSLALFSPLWGHLADRYSRRLMLLRANFAGAAIVM
                     LMGLVRSVEALCLLRLMQGMFTGTMTAAQTMVSVQAPPHRSGQALGALTAAVFSGSLT
                     GAFVGGVVADWFGYRAAFLVGGAFLLVSGLLVALWTTEDAPGAPHAPEARAADERLAG
                     QGVLPVLLLFGLLSFVRQFDVSFLPLLVQDIHGGLAGASIRTGALNASGGIAGLLAAL
                     VVGRVADRSTPARLAVLLTLGAGLLMAPQAWTRTFGFLFPVRFAMVFCTGALDPVMQI
                     WLTRITPVRRRGAVFGWAGTMRSAGWFLAPLGSGLVAARYGIRSIFLVGGLLFALVLP
                     AIRWAERSRREPVDPTGPRPTR"
     gene            complement(11772..13049)
                     /locus_tag="PK634_06275"
     CDS             complement(11772..13049)
                     /locus_tag="PK634_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011174699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOW97378.1"
                     /translation="MKPSLVPVIIFILCYALFVVLPKRRSWIALGGGALLVLTGSLGW
                     RTALTETIQWNVIALFFGTLVLAEMFMQSRMPAVMAEFLVDRTRTVRGALLAVCALSG
                     GLSMFVENVAVVLLVAPVALSLSDKLKLSPVPLLIAIAISSNLQGTATLIGDPPSMIL
                     GGYMKMSFNDFFVYQGKPGIFFAVQVGALASLAVLAWQMRAHREPTVIVPQERIRSRV
                     PGLLMLALILGLSVTSVFDPDFKWLAGTLTLVLAAVGLLWYRFRARWQSLRELVGTLD
                     WDTTLFLIGVFVLVGGLSESGWLDVFASWISAHLGGCLFGAFAAIVLVSVALSAFVDN
                     VPFLLAMIPVVQKVADHLEAPVPVLMFGLLIGACLGGNITPIGASANVVAIGLLRKRG
                     YVVTFREFMAVGVPFTVAAVIAACAFTWWVWAP"
     gene            13164..13658
                     /locus_tag="PK634_06280"
     CDS             13164..13658
                     /locus_tag="PK634_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOW97379.1"
                     /translation="MDIFKAIEARHSSRKFTRAPVPRAHLEVIADAGRRAPTARNEQP
                     WEFVAVTDRARLKELAGLAENGKFIADAGACLAVVSRPSKYYLEDGCAATENILLAAT
                     ALGHQTCWVAGDKKPYAPAVCRLLGAPEGFLLVSLVAIGKAEATGGRAPKRALDEVLH
                     WEKF"
     gene            complement(13665..14927)
                     /locus_tag="PK634_06285"
     CDS             complement(13665..14927)
                     /locus_tag="PK634_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOW97380.1"
                     /translation="MPTHRSQESGRDVIVVGGGPAGLMAALSAARRGASVRICEQLER
                     PGLRLMASGGGRGNLTNTLAPEAMMARFGRRGRFIQPALAGLGPVSLRRLLADLGVPT
                     FSPDGFHVYPSSESAADVQQALVRAGEDAGVDFRLACPVQSLAIEKGAAIGVHTSGGL
                     VPAGRVILTTGGRSYPRLGGGEQGYALARQAGHTVTLLLPALAPLVIAEPWPPSCAGA
                     ALPDVECTLSREGRSAPAARGALLFTHRGLSGPAALDLSGDVSARLQAGAPVAAFLNL
                     APGRRPSDWLQEIGQWAHVGGGKTVVRWLDDFLPRSVAAALAGACGLPDSLTASRISQ
                     EQRRRLADHLAGWPVTITGTGGFEQAMVTRGGVALDDIDPHTLSSRLVKGLHFAGEIV
                     DLDGPCGGFNLQWAFSSGWLAGHSAAGA"
     gene            15041..17833
                     /locus_tag="PK634_06290"
     CDS             15041..17833
                     /locus_tag="PK634_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW97381.1"
                     /translation="MKVPFPWRRNPVGPAGRRGRAWCLAAGAALAWGLGPGLFADSQS
                     DTTAFLQGKKVLVLCSYHYGMRWTDSICAGIRSVFDDPQGKWHVLQYEFMDAKRISSP
                     EYFEELARLFAMKFKPREFDVIIASDDDALSFLLQYRERLFESTPVVFCGVNYFQPEM
                     LANQSDITGVVESFDLVATLDAALMLHPNAKLVYVVNDQSSTGRANNRRIEDVMPRYA
                     ERAHFRFTGPASMAELLEEIRGLPDNAVILLMTFNRDRLGEVFRYRDAARLVTGATPL
                     PVYGVWAFYLGDGIVGGMLTRGETHGRAAAEMAMRILGGERAESIPVILQCPNQYMFD
                     YNQLRRLGINVSKLPPGSLVIHKPEPMYRFRKQTVWLVVSALAVLGLLVLAHAINVNK
                     RLQAQHALQEANEKVRRESADRRQAEQERERLAAAVAQMSDGVIISDSQGVICYVNPA
                     LERMTGYQSAELLGQSARIFGRPEDVRQVRQVLEALRHSENWRGRMRTRRKDGTLRDE
                     DVLICPVKDSAGRVGHYLAIRRDVTESVALQNQLMETQKIETVGEIAGGIAHDFNNML
                     TVILTSAQFLADGVAGDPKLRKDAGEIVRTAKRASELARELLTFCRKQPLELKARNVN
                     EIVFGLEAMFHHILGERVRLVLDLTREACIAQVDKGRLEQALANLVMNARDAIPAQGV
                     VTLRTALARVEDEPAAEFLEPPMDAAGRFVRISVQDTGTGIPENIRQRIFEPLFTTKP
                     AEQGTGLGLSVVYGIIKQHGGRLALATQVGRGSTFKIYLPAAYIPPANLAATGAIAPV
                     PSGRESILLVEDERSVRDVIARIMTHLGYKVTAADCAESALELMDRTTGPLDLLFTDI
                     VMPGMDGVSLAREVLRRRPGLRIVFASGYSQEHLSKDPKFACIPLLKKPFEAGDLARL
                     IRRVLDGPEQRPGPAA"
     gene            17859..18842
                     /locus_tag="PK634_06295"
     CDS             17859..18842
                     /locus_tag="PK634_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOW97382.1"
                     /translation="MKEAPRFLRAGWMLCWLLLGATAALQVAAWNHARAFLRFQHRGA
                     SPTPIESLGARDKVRLLFRGAAKPRPENRRAPADVGLTAETVRFSAADGTGLEAWFVN
                     APGDKPPVLFFHGYASARSSLLDEAAMARDLGHPVLLVDFRGSGGSDGNSTTLGYLEA
                     LDVKAAVSWIRTRLPGRPACFLYGQSMGAAAVLRAVTIHDVAAGGLVLEAVFDTMLNA
                     VRNRFKAMGWPAFPAAESLVLWGGVQCGFNGFRLNPADYARSVKIPTLVLYGDRDLRL
                     TLKQARRVFDRLAGPRLMVEFPRASHSSCLASDPARWRDTMSRFLSGEATP"
     gene            19027..20319
                     /locus_tag="PK634_06300"
     CDS             19027..20319
                     /locus_tag="PK634_06300"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOW97383.1"
                     /translation="MKEIGIGLLGFGTVGAGVVEGLQQNGKLLEERLDLRLALRRLAD
                     LDLDSDRGVKADRALMTRDAGEAIRDPNVDIVVELIGGVTKAKEFIRQALELGKPVVT
                     ANKALLAEAGAELHRLAAEKKTALYFEASVGGGIPIIKAVREGLVANRITSVYGILNG
                     TCNYILTRMEEEKLPFEQVLKAAQQAGYAEAEPSLDVDGHDTAHKAVVLASLAHGFPV
                     PMSAVHVEGIRNISAGDISYAAEMGYRIKLLAVIKQGGNGVEARVHPTLVPHAHPLAA
                     VSGVFNAVLVSGDIVGDTLYYGRGAGRRATASAVLADLADAARDLSSGARRPPVLRRE
                     SGKLRPIGEIVTRCYLRLSLLDKPGVLALVAQALGRKGISIASVMQKETRVGQHVPVL
                     VVTHSAPESAFRAALAEIDRLEVVGAPTVRIRIEDFQE"
     gene            20374..21726
                     /locus_tag="PK634_06305"
     CDS             20374..21726
                     /locus_tag="PK634_06305"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOW97384.1"
                     /translation="MKVAKFGGTSLADAGQILKVLEIVRADPDRRIVVVSAPGKRSKD
                     DTKVTDLLIACAKAALARGEAEAELALVVHRFAEIGRELDLPAALLTDIEQDLRGRLA
                     ADRSHKGLFTDAVKAAGEDYSARCVAAAFTRKGVPATYTCPREAGMVLSDEPGNAQVL
                     PESYDKLAALRDRPGVTIFPGFFGYTKAGRIVTFPRGGSDITGAILAAAVKADAYENF
                     TDVDSVYSADPRIVPDARPIELLTYREMRELAYSGFGVFHDEAILPAVHANIPINIRN
                     TNRPSAPGTMVVPERPAHTGGVVGISSSDGFCTLFLSKYLMNREIGFGRRLLQILEEE
                     GLSYEHAPSGIDNTSVILRDHQFSPEKERHVVGRIQKELAADDVEVERGLALLMVVGE
                     GMRYTVGLASRATQALAGAGVNIEMMNQGSSEISMMFGVKAEDRKKAIKALYHAFFAA
                     "
     gene            complement(21745..>23490)
                     /locus_tag="PK634_06310"
     CDS             complement(21745..>23490)
                     /locus_tag="PK634_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOW97385.1"
                     /translation="PDAVVNYPGTAFPYLAANLVFTNDSNLKTQVAADGQPYPLVTNL
                     IARSCTIAVHGDTVGVVGVTTPELATLSSPGGVIVLTNVAAEAQAAVHALTNAGLNKI
                     ILLAHLQQLENELDLAGRLAGVDILIAGGSHTLLAKPGDRLRAGDTRYGDYPLVRTDL
                     VGRVVYVLNTAANYRYVGRFVADFDAGGFITNVSTASGAYATDDQGVADTGGYPPAPA
                     VTGVVAVLAGIIDAKDGNLFGRTAVYLNGIRESVRTEETNLGDLSADANLYRAGLTDP
                     AASLSLKNGGGIRDSIGAILSEGGGSIRVPPLANPRVGKQDGDVSQLDIENALRFNNS
                     LSILTITAQDLRDAVEWGVAGSGTPGQFPQVGGLEFSFNPTNAPMTYSTTEDGTPTNI
                     LFPGERLRSLVARRADGQRDLVVENGALVGDSNRTFRLVTLEYMADGGDRYFALTRGI
                     QRTNLVPADAVKTFTTDGGEQKALADYLWALGECSRPDTEASADGRIQRLNAREDDVL
                     KPGIVSLARAAGVVRVDFSTLPGKSYQAESAPALAGPWAAIPALPPVAGNGYDTNVVF
                     SPGGPAEQFFRLEME"
BASE COUNT         3880 a         7675 c         8153 g         3782 t
ORIGIN      
        1 cgaggccctg gcgacgcttt cccgcgggga cgtggatctc gtcctcctcg atgtactcat
       61 gccgcagcgc gacggcatct cgctgctgcg ggagatcata gatagcttcc cgcacctggc
      121 ggtagtcatg gtcagcgggt cgacctcggt gcggccggtc gtcgaatcga tccaggccgg
      181 cgcgctggac tacctcccga agccgttcga ggtggacgat gcccggcgca tggtcgcccg
      241 cgccctcgag cgcgccgcgc tgaagaggaa tgtggagatc ctgcaacgcg aggtcctgcg
      301 cgaattcccc gtgcaggata tcgtcggtca cgcgcccgcc tttctcaagg cgatcgagaa
      361 cgtccggaag gcggcggaaa ccagcgccac ggtgctgatc gagggcgaaa gcgggacggg
      421 caaggaattg atggcgcgcc tgctgcactt ccagagcaac cgccgggagg agccgttcgt
      481 ggcggtccat tgcgcgtccc tgccggagac gctgatggaa agcgagctct tcggccacga
      541 gaagggcgcg ttcacgaatg ccgaccggag acgcctgggt cgtttcgacg tggccggggc
      601 ggggaccatc ttcttcgacg agatcggcga aatgacgctg gccacccagg tcaagctgct
      661 gcgggttctc caggaacacg agtatatgcg cgtggggggc acccagacga tcaagacgcg
      721 cgcgcgcgtg gtggcggcca cgtcccgcga cctccgcgcc gaggtggagg cccatcgctt
      781 ccgggacgac ctgttctatc gcctcaacgt cgtgccggtc cggctccccg cgctccgcga
      841 tcgccgggag gacatccccc ggctggtgga gcattacttc accgcgtttc gcgcggcatt
      901 gaatgcgcgc gcccgcggat tctccgccga cgcgcggtca ttgattgggc aatatgactg
      961 gcccggcaac gtgcgcgaac tccgcaacct ggtcgaacgg gtgctggtgc tgcacgggca
     1021 gcaggaggaa atcggtccgg aatgcctgcc cgatgaaatc cgactccggg aacgcggcgc
     1081 gggggtggcg ccgctctcat tggaagaggc cgtcaacgat ttcgagcggc gcatggtcgt
     1141 caaggctctt gccgaggcgg gcggcgtgca gacacgggct gccgaacggc tgggcaccac
     1201 gcggcgaatc ctgcggtatc gcatggagaa actgaatatc cccaggaacg gggactagcc
     1261 agcccggtgc cgcgttgtcg agttcccttc acctgtgtta ctctgcttca aaagccggga
     1321 ggccgactcc atgaaatttt accgcaagga actctggttc aacacgccag cgcggcgggc
     1381 gttcgtcaac atcacgccgc aggtcgagga gtgcctgcgc gagagcggca tccgagaggg
     1441 gctgctgctc tgcaacgcca tgcacatcac cgccagcgtg ttcatcaacg acaacgagag
     1501 cgggctgcac gccgactttg agaagtggct ggagaagctc gcgccggaaa agccctacac
     1561 gcagtacgcc cacaacggcg ccgaggacaa cgcggacgcc cacctgaagc gcaccgtcat
     1621 gggccgcgag gtggtcgtcg ccgtcagcgg cggcaaactg gatttcgggc cgtgggagtg
     1681 cattttctac ggcgagttcg acggccggcg ccgcaaacgc gtgctggtga agatcatcgg
     1741 ggaataaagg agatgccatg cgaaagacct ggatcgcgct actgttgatc tcggcgctgt
     1801 cggccggcgc cgcggaaagc tgggtttcgt tcgtgatcac tgcgaacgat ctcccggaaa
     1861 acggctgggt gcacatcacg ggcaaccatg aaaagctcgg aagttggaac ccgtccgccg
     1921 tgccccttca gaagcgcgcc gacggtttct gggtgctcaa ggtgcccgtc gagaccggct
     1981 cgacgctgga atacaagttc acgctcggct cgtgggacac cgaggcgctg gccgccgacg
     2041 gctccgtgcc gccgaaccac gtgctccgcg tcggcggtga tacgaccgtg cgcgaggtcg
     2101 tcacccggtg gccgacgccc cgcgatccgg ccgcgccgaa gtacacggga accgtggaat
     2161 accacgccgg catggcgggc gaaggcatcc tgccgcgcga cgtgctggtc tggctaccgc
     2221 caagctacgc gaccgatccg gagcgccgct atcccgtcct ctacatgcac gacggccagc
     2281 aggtcttcga tcccgccacc tccacccacg gcgcggactg gggcgtggac gagaccgcga
     2341 cgcggctgat cgccgagggc cgcctgcagg agatcatcgt cgtggccgcc acctgcacca
     2401 gcaaccggtt cccggagtac tcggagacgg aagcgggctc cgcgttccgc cgattcctcg
     2461 ccagggatct gaaggcgttc atcgatgcga agtaccgcac catgccggac cgggcgcata
     2521 ccgccgtcat gggttcctcc atgggcgggc gcgtctcctt cctgctggcc tgggaatacc
     2581 cggatgtcta ctcgatggcc ggctgcctct cgccgtcgtt ctcggacgag acgctggccc
     2641 tcgcccgggc gaagcaggaa gacccgcccg cggtccggtt ctacatggac aacggcggga
     2701 tcggcctcga agaaaggttg cagacgggca tcgatcaaat gctgcccctg ttgcaggaca
     2761 tgggatttga acagggggac aacctggtct ggttccgcga catcgcggcc gagcacaacg
     2821 aggcggcgtg ggcgaagcgc gtctggatgc cgctgctcta tttcttcggc cagggcccgc
     2881 aggactggat acgggaattg ccgccgatca ccggcgggga gccctccgcg ccgtagcatg
     2941 ccgcgcatcg ccagagtggt cgtcgaggtc gcgcgcaacc gcgagtttga ctatctgatc
     3001 ccggcccggc tcgaggggca gctccgggtc ggcatgcagg tcgtcgtgcc gttcggccat
     3061 tccgaggccc gcggctatgt cgtcggcttg tccgacgagg cgcggcaccc gaagctcaag
     3121 gaaatccgcg acaccgtcgg ccaggccgtc ctcgtggacg agcgcctgct gcagctggcg
     3181 cgctggatga gcgaatacta cctggcgccc gtcgagcgga ccatccacac ggtcctgccc
     3241 gcgccggtcc ggagaaaagg gaccggcttc aaggagcggc tcctggtcca gccggccgag
     3301 gcggcgatgg acgacctggc cgtggagcgc ttgcgggcga aggccccgaa gcaggccgcc
     3361 gtgctggacg cgctccgggc gggcggtccg gcgttcctca acgacctggc gcgcgccgcg
     3421 aagaccacgt ccgccgccgt ccgggcgctg gagaaaaagg gatgggtgcg gatcggccgg
     3481 caggcggtcc accgcgatcc gctggccggg caggagctcc tgcgcacgca gccgctggcg
     3541 ctgatgccgc agcagaagga ggccctcgac ctcgtgtgcc gcgccatgga caccctggag
     3601 ccgccggtcg tgctgctcta cggcgtcacg ggcagcggca agacggaggt ctacctccag
     3661 gccatcgccc gcgcgctgga gaaggggcag ggggccatcg tgctcgtgcc cgagatatcc
     3721 ctgacgcccc agaccatcga acgcttccac ggccggttcg gcgacactat cgccgtgctg
     3781 cacagctccc tctccgacgg cgagcggcac gacgagtggc accgggtccg ccagggccgg
     3841 gcgcgcgtgg tcatcggcgc ccgctcggcc ctgttcgcgc cggtggagaa gctcgggctg
     3901 attgtcgtgg acgaggagca cgagccgacc tacaagcagg aggagacccc ggcctaccac
     3961 gcccgcgacg tcgccgtgat gcgcggccac ttggagggct gcgcggtgct gctgggctcc
     4021 gccacgccct cgctggagtc gttccagaac gcccgcatcg ggaaatacgg actggcccgg
     4081 atgccggcgc gcgtggacca ccgcaccatg cccgcggtgc gggtggtgga cctgcgcgag
     4141 gaggccgagc gcgcggggcg gccgaatgtc ttttcggggg acctggtgga cgccatccac
     4201 tcgcggctct cccgcgccga gcagaccatg ctcttcctca accgccgcgg cttcgcgacg
     4261 tccctggtct gcccccagtg cgggtacgtg gcgcgctgca acgagtgcag cgtggcgatg
     4321 acctaccacc gggagcccgg cgcgccggaa atcctgctct gccacgtgtg cggggagcgc
     4381 cgcatcgtgc ccgcgcggtg cccgaacgac aagtgccgca ccccggcctt caagttcgcc
     4441 ggcgtcggca cgcagcgcat cgaatccatc atctcccggc tgttccccga cgcctgcgtc
     4501 cggcgcatgg attccgatac gactggccgg aaggacgcgc accgggagat cctcggagaa
     4561 ttccgcgcgg ggcgcatcga catcctcgtc ggcacccaga tgatcgccaa gggcctgcac
     4621 ttcccgaacg tcacgctggt cggcgtgctg tacgccgact tgagcctgca catgccggac
     4681 ttccgcgcgg ccgagcggac cttccagctg ctgacccagg tcgccggccg ggcgggccgc
     4741 gggcaggcga ccggcgaggt catcatccag acctacacgc cgtttcaccc ggccgtgcag
     4801 gcggcccgtc ggctggacta cgaagggttc ttcgaccagg agatcgagtt ccggaaagag
     4861 ctgggctatc ctccgttcgg gcacctggtg tgcctgcatc tccagggccc gggcgaggcg
     4921 aaggtccagt tcgccggcga ggcgctcgtt cgcgccctga agccgaagct gccggcctcc
     4981 gtgaaactgg ccgggccaac ccccgcgccg ctggcccggg cccgcgggca gtatcgctac
     5041 caggtcctcc tgcgagcgcc gtccacccgg agcatgaccg ccccgctcaa ggccgtgctg
     5101 gaaaccttca agtggcctga ggacgttcgc tgcattgtgg acgtggacgc ggtctcgctg
     5161 ctctagtatt cctcatagct catccaatcg gaccggccct cgcggaaccg cccttgcttt
     5221 tggctattcg ttcgatatcc tgatcctctg aaggggggct gcaattccag cagtcaataa
     5281 acatgctggg cgtacatgac ggcctcgcgc ctattcacca cacgcagcgg cgggctcaag
     5341 agctgcatcc aaggcccggt cagcccggac cgcgcctttt ggattcatcc gaacatcgtg
     5401 ttagaaagcg aatcatcgtg aagagaatgc gatcagggca tggcggcggc gttcgcgacg
     5461 ggttgatggc cctgctcttg ctgggctgcg ccgcgccgct ggccgcgcac gtacttccgt
     5521 ttgaggaaga cgacacgttg gaagagatcc ggagcaagat ccaacacaac ggttatcggt
     5581 tcaccgtcaa caccaaccgg ttcacaaaca tgactcccgg ggagcgccgg gcgatgttag
     5641 gcggcaggcc gtgggaagga ccggaacctg cgccggccgt ccagttcaag gccagacggg
     5701 ctgaagacct gccgtccgca tacaatataa ccaatctcaa cggccatacc tacattggtc
     5761 cgattcacga tcagggcttg tgcggctgct gctggatttt cggagcgctg gccgccgccg
     5821 agagcgcctt caatctggat agaggccgtt acgacaacca gtgcgtggat ttctccgagc
     5881 aatacgccat ggactgttat tcgatgcccg attctccgta tcggcacata aacgcctttg
     5941 gttgtgaagg cggctcacac tggatagccg ccgagcggct ggtcgcgtac ggggtgacgg
     6001 aagaaacgaa tgcgccctat gagatgatgc agctcgactg ggaacgacac atgggcaaac
     6061 ccctgacccg tttcaaatcc tggggaagac ttcccgtaaa cgacatcccg gccatcaaga
     6121 ccgccatcat gacctatggc gccgtggcgg cgagagtggc ggcttcgccc gcttttacat
     6181 tttatagcag cggcatcttt gaggatgcca acaccaacgc cgatctgtca tcctgcaatc
     6241 acgtggtggc ccttgtgggt tgggacgaga acggcgggga cggttactgg attttgcgca
     6301 actcgtggaa cgtcgattgg ggcgaaggcg ggtatatgcg cattaaatac acctccgccc
     6361 atgccgccgg cggggtgatc tacatcgaat acgacccgga tgaagtgtcg gccgttcccg
     6421 tgtgcctgag cgcgagcgac ggcgcataca cgaatcaagt gcgtttgacc tggtgggcgg
     6481 cggatggcgc cgaaggatat cggatattcc gcgataatgg cgccgatacg aatgacgcga
     6541 tacagattgg gataacctct tccaccaact acgatgacac cagcgcggtg gccggcacga
     6601 tgtatagtta ctggttgaag tccaccaacg ggtgcggaat cagcggcttc agttacttcc
     6661 aggattccgg ctggcgcgct tcgccaaccg gggcgatcgg agtgctgccg ccgatgttaa
     6721 gctatgcggt cgcctatggc agcagtaatc cggcggcaca gagctttatc gtcaccaact
     6781 cgggcgcgat cggattcaat ttcacgaaca gcacgacgta ttccgccgat gcttccggct
     6841 ggtggcaggc cgtgccggga acaggcagcg tgacggcttt cgcctccttg gcgttgaccg
     6901 gtacggtgga cgtggccggt ctgaatgccg ggacgtatta tgccaacagc atgatcgctt
     6961 cttccgaggc ggacaacagt ccgtacagcc tggtggtggc gttgacggtg aacaagggcg
     7021 atcagatcat cacattcccc aaaattcccg accaggtggt gaccaacacc gtcggcatca
     7081 gcgcgaccgc gtcgtccgga ctcccggtcg tcttgcgggt cgtgtccgga ccggccgtgc
     7141 tggacggaac aaacctatcc tttaccggcg ccgggtgggt gaacgtgaag gcctatcagg
     7201 ccggcgacac gaattggaac gaggtgtacg tgtaccaggg gttccgggta ttcggccttt
     7261 acacgctgac gatcgagtcg gcgcacggaa cgtgcagtcc ggttgtcggg atttacacga
     7321 acgtcgaaga cagtgtgttg accaacacgg tcaccggcgc cgatatccac ggggatacgc
     7381 aatacgtgtg tgccggctgg gccggcagcg gcagcgttcc gcctgaaggc gggacaacgg
     7441 actgtacctt cacgatcacc agcgattcgg tcctgacgtg gctttggact acgaactact
     7501 ggttggccag caccggcggg ctgcacggct ccgtcaccgt cccttccggt tggcagccgg
     7561 ccggcgctac gaccgcgatt acggccacgg cggacggata ctatcacttc acgaactgga
     7621 cgggggatgt gagcggtccc gacgtgcagg ccaatccgtt gaatctgttg atagatgcgc
     7681 cgaaggcggt gacggctcat ttcgcggaga acctgacgac gaacaccggg acgccggagt
     7741 ggtggctggc gcagtacgga ttgacgaacg gcggtgtttc attcgagcag gcggcggcgg
     7801 atgacacgga ccgggacggc gaggcggctt gggaggaatg tgtggccggc acggacccga
     7861 ccaatcgggt ctcggcgctc gggatccatg acattgagtt atccggcacg ctgattgaga
     7921 ttgaatggcc gagcgtgagc ggtcggctct attcggtttt ctgtgcgacg aatctgccgg
     7981 tgggcgagtt caatctgttg gggacggcgt ccaatctgca ggccacgccg gatgtgaaca
     8041 cctacaccaa cacaacggac acgaacgtgc cggtgttgtt ctatcgcatc ggtgtccgca
     8101 aagggctgga atgagaacaa cctttttacg ccggaagcgg ccaaaagttt cccatggttg
     8161 gaaatccgga ggggtcccat gtcgcgcgtg aaaatcgagc tgcccgagtc gctgcctttc
     8221 gtcaccgagc tggaggtccg catcggggac gtgaactatg gacggcatct cggcaacgac
     8281 gccgtgctcg gcctgctgca cgaggcccgg ctgcggttcc tgaagcagaa cgggttcacc
     8341 gaggaaaacg ccggcggcgc ggcgatgatc atgctcgatg ccgtgatcgt ctatgccgcg
     8401 caggcgttcc acggcgaccg gctgcgcgcg gaggtcggcg cgggggattt cggcgcctgc
     8461 ggctgcgact tctactaccg gcttacccgg atcgcggacg gccgggagat cgcccgggcc
     8521 aagacggccc tcgctttctt cgactacgcg gcccaccgga tcgcgcggat gccggcggaa
     8581 ttcaaggcgc ggctggcgac gccggcttga gcgccgcgag cgcgtccagg tcgatcttct
     8641 tcaccgcggc ctggagggcc tcccacgcga ccttgtagcc ggtggccttc accatgaagc
     8701 ggccgcccgc gaggatctcg atcgtgccgc gctggtcctc gttgtcgtac tcctcgcgcg
     8761 ccttgtggct accgtacgcg gtcgtgcgct cgaaaccggt gtcggtctcg cggtcgatct
     8821 cggacgaggc ccacgcggcc tgggcgaagc tcatcagccc gcccatgccg ccgttgtcgc
     8881 tgacgttgat ctcgatggaa gcgtcgccct gctcgtagcg ggcctcggcc tgggacacgg
     8941 tcatgcccat cgcggtgttc ttctcgccgg tggcgctcgt ccgggtgaag ccgtcgagcg
     9001 attcggggag cagggccttg agcgcgcgga agtccacgac cggcgcgctg ttgctggccg
     9061 ccgcggtcat cgcctgcatg gccttcatga actcggccat gtcggcctgg gacggttgct
     9121 gcgcccggac gctgccctgc atcagaagac ctgctgccgc gataacgaga agtcgtttct
     9181 tcattgaatg ctattctccc gtggttgagc cggccgaact ataccgcacg aaggggatgg
     9241 gcgcaaccgc accggccttg cgatccggcc ttccggcgat tagagtcggg gcatgcggca
     9301 cccgaaggcc atcggctggg aggacaagtt gagggcgctc ttcgaccgta tcgacgaccg
     9361 gctggaggag cgctacggga agcagtaccc cctgcattcc aaccgcccgc cgcgcgggac
     9421 gacctccagt cgcgaaatgg acggcctgtt cgacatcggc gccgcgttct ccgccgggta
     9481 cgggtcggcc gtcgggcgcg gctacgtggt ggagatccgc atggccacgc ccgcgcgcgt
     9541 gccgccggcg gtcatggagc ggatcgagca ggaggtcgtg tccatgatcg aggcggaagt
     9601 gaagacggag ttcccgggac gcgatctgaa ggtcagccgc gacgggcccg tgtataagat
     9661 ccacggagac ttgagcctcg gcgaggcgtg aaacggtagg gcgggttcgc cgaacccgcc
     9721 gggactccgg cgcgttgggc caacgcgccc tgccgctaca gcagcagggc atcgagctcg
     9781 gccagctttc cgatcgtgac gtcgggttcg aagtcgccgg gcttgcgggg cagtttctcg
     9841 ggcgtttccc actggcgcag catcaccacg ttcagcccgg cgcgcttgcc gccctgcacg
     9901 tccgccaccc agttgtcgcc gaccagcacc gcctggtccg cgagcacgcg caggtcgtcc
     9961 aggatcgtca ggaacggcac cgggtgcggc ttgacgagat gcacgtcggc ggacacgacc
    10021 acggatttga agtaggcgtc gagcccggtc ttggccaggc tttcccggat ggcgtcgccg
    10081 tcggggtagt tcgagagcag gccgagccgg taccgctgcg cgagccggtc gagcaggtcg
    10141 tacacctcgg gctcggcgcg gacgatgcgg acgaacacct cgaagcgggc gcgcaggatt
    10201 tccgcgagct gctcgggcga gggatcgagg ccgtagagct cctggatcat ggccgtcgtg
    10261 atctcgacga ggtcgttctc gcggtagagc gggggatcgc cggcgtacgg ggccaggcgg
    10321 ttgcggtcgc ggatggcgga cagtttctcg aagtcgggct tgccgaagtg cttctcgagg
    10381 acgtcggcga gggcggcgtc gcagacgacg acctgcgtgc ggctgaattc cacgaccgtg
    10441 ttgccgtagt cgaagatgac cgcccggatg cttttcttgt cgaacgccat ttttccgcta
    10501 ccttctttcc cgccgcggct ttccagaatg ccggaggcgc gcggcgcaat caatggcaat
    10561 ccgatgggtc aggcgcgttt ttcacgcggg ctgggaacgg aacgtcgtgg tcaccggcgt
    10621 gtcacagttt ttctccatca tgggctttgc gttcgccctc ccgttcgcgc cgtactacat
    10681 gcaggaccag ctcggcgtga cggacccggc gcggctgaag ttctgggtgg cgctcttcgg
    10741 cgcggccacg cccctgtcgc tggcgttgtt ctccccgctg tggggccacc tggcggaccg
    10801 gtatagccgg cgactgatgc tgctgcgggc caacttcgcg ggggccgcga ttgtcatgct
    10861 gatgggcctg gtgcgcagcg tggaggcgct ctgcctgctg cgcctgatgc aggggatgtt
    10921 cacggggacg atgacggcgg cccagacgat ggtctccgtg caggccccgc cgcaccggag
    10981 cggccaggcc ctcggcgcgc tgacggccgc cgtgttctcc ggctcgctga ccggggcgtt
    11041 cgtcggcggg gtggtcgccg actggttcgg ataccgtgcc gcgtttctgg tcgggggcgc
    11101 attcctactc gtcagcggcc tgctggtcgc cctctggacc accgaggacg cgcccggcgc
    11161 gccgcacgcg ccggaggccc gcgcggcgga tgagcggctc gcggggcagg gcgtcctgcc
    11221 ggtgctgctg ctgttcggcc tcctgtcgtt cgtccggcag ttcgacgtgt cgttcctgcc
    11281 gctgctcgtg caggatatcc acggggggct ggcgggcgcg tcgatccgga cgggcgcgct
    11341 gaacgcgtcc ggcgggatcg ccggcctgct ggctgcgctc gtggtcgggc gggtggcgga
    11401 tcgctcgacg cccgcgcggc tggcggtgct gctgacgctc ggcgccgggc tgctcatggc
    11461 cccgcaggcc tggacgcgca ccttcgggtt ccttttcccc gtccgcttcg ccatggtgtt
    11521 ctgcacgggg gcgctcgacc cggtgatgca gatctggctg acgcggatca cgcccgtgcg
    11581 gcggcgcggc gcggtgttcg gctgggccgg cacgatgcgg tccgccggct ggttcctggc
    11641 gccgctgggc agcggcctcg tggcggcgcg ctatggaatc cggagcattt tcctggtcgg
    11701 cggactgctg ttcgcgctgg tgctgcccgc gatccggtgg gcggaacgct cgcggcggga
    11761 acccgtggat cctacggggc ccagacccac caggtgaacg cgcaagccgc gatgacggcg
    11821 gcaaccgtga acggcacgcc gacggccatg aactcgcgga acgtcacgac gtagccgcgt
    11881 ttccggagaa gcccgatggc cacgacgttg gccgacgcgc cgatcggcgt gatgttgccg
    11941 ccgaggcagg cgccgatcag caggccgaac atcaggaccg gcacgggcgc ttcgaggtgg
    12001 tcggccacct tctgcaccac ggggatcatc gccagcagga agggcacgtt gtccacgaag
    12061 gcggagagcg ccacggacac cagcacgatc gccgcgaagg ccccgaacaa gcagccgccg
    12121 aggtgcgcgg aaatccatga cgcgaagacg tcgagccacc cggactcgct caagccgccg
    12181 acgagaacga acacgccgat cagaaacaac gtcgtgtccc agtccagggt gccgaccagc
    12241 tcgcggaggc tctgccagcg ggcgcgaaag cggtaccaca gcagcccgac ggccgccagg
    12301 accagggtca gcgtgccggc cagccacttg aagtccggat cgaacacgga ggtcacggac
    12361 aggccgagaa tgagcgcgag catcagcagg ccgggcaccc gtgagcggat cctctcctgc
    12421 gggacgatca cggtcggctc gcggtgcgcg cgcatctgcc aggccaggac cgccagcgag
    12481 gccagcgccc cgacctgcac ggcgaagaag atgcccggct tgccctggta cacgaagaag
    12541 tcgttgaagc tcatcttcat gtagccgccg aggatcatgc tgggcgggtc gccgatcagc
    12601 gtcgcggtgc cctggaggtt cgagctgatc gcgatggcga tgagcagggg gacgggggag
    12661 agcttgagct tgtcggatag gctcaaggcg acgggcgcga cgagcagcac gacggccacg
    12721 ttctccacga acatcgagag gccgccggac agcgcgcaga cggccagcaa cgccccgcgg
    12781 accgtgcgcg tgcggtccac gaggaactcc gccatgaccg caggcatgcg cgactgcatg
    12841 aacatctcgg cgagcaccag cgtgccgaag aacagcgcga tcacgttcca ctggatcgtc
    12901 tccgtcagcg ccgtgcgcca gcccagcgag ccggtgagga cgagcaaggc gccgccgccg
    12961 agggcgatcc acgagcgtcg cttcggcagc acgacgaaca gggcgtagca caaaataaaa
    13021 ataatcaccg gcacgagcga tggtttcata gcgtgtcgcc agtctggagg aaccgtttgc
    13081 ggcgcgtcaa taaaaacacg acgcgggatt gcgcggcggc ccccggcgcg ctatgaatat
    13141 ccaggaaccg gaggaggacg gacatggaca ttttcaaggc gatcgaggcg cggcacagtt
    13201 cccggaaatt cacgcgcgcg cccgtgccgc gcgcgcatct ggaagtgatc gcggacgcgg
    13261 ggcgccgcgc cccgacggcc cgcaacgagc agccgtggga attcgttgcg gtgaccgacc
    13321 gcgcccggct caaggaactg gccggtttgg ccgagaacgg caagttcatc gcggacgcgg
    13381 gcgcgtgcct ggcggtggtg agccggccgt ccaagtacta cctcgaggac ggctgcgcgg
    13441 cgacggagaa catcctgctg gccgcgacgg cgctgggcca ccagacctgc tgggtggccg
    13501 gcgacaagaa gccctacgcg ccggcggtgt gccggctcct cggcgcgccg gagggatttc
    13561 tcctggtctc tctcgtggcc atcggtaaag cggaggccac cggcggccgg gcgccgaagc
    13621 gggcgctgga tgaagtgctg cactgggaga agttctgaag ccggtcacgc gccggcggcc
    13681 gaatggccgg cgagccagcc gctggaaaag gcccactgaa gattgaaccc gccgcagggg
    13741 ccgtccaggt ccacgatttc gcccgcgaaa tgcaacccct tgacgagacg gctcgagagg
    13801 gtgtgcggat cgatgtcgtc cagggccacg ccgccgcgcg tgaccatcgc ctgctcgaag
    13861 ccgccggtcc cggtgatcgt caccggccag cccgcgagat gatcggccag gcgccttcgc
    13921 tgctcctggg aaatgcgcga agcggttagc gaatccggca gcccgcacgc gccggcgagc
    13981 gccgcggcca ccgagcgcgg caggaaatcg tccagccacc gcacgacggt tttcccgcct
    14041 ccgacatgcg cccattgacc gatctcctgc agccagtccg atggtcgtcg gccgggcgcg
    14101 agattgagaa aggcggctac gggcgcgccg gcctggaggc gtgcgctgac gtcgccggac
    14161 aggtccagcg ccgccggccc ggagaggccc cggtgcgtga agagcagggc gccgcgcgcg
    14221 gcgggggcgc tccggccttc gcgggagagg gtgcattcca cgtccggcag cgccgccccg
    14281 gcgcaggaag gcggccacgg ctccgcgatc acgagggggg ccagggcggg aaggagaagg
    14341 gtgaccgtgt gaccggcctg ccgggccagg gcgtatccct gttcgccgcc gcccaggcgc
    14401 ggatagctcc ggccgccggt cgtcaggatc acgcggccgg cggggacgag gccgccggag
    14461 gtatgaacac cgatcgcggc gcccttttcg atcgccagac tttgcaccgg gcacgcgagg
    14521 cgaaaatcga ccccggcgtc ctcgccggcg cggacgaggg cctgctgcac atccgccgcg
    14581 gactcggaag acgggtacac gtggaatccg tccggcgaga aggtcggcac gcccaggtcg
    14641 gccagcagcc ggcggagaga caccggcccc agtccggcga gggccggctg tatgaatcgc
    14701 ccgcggcggc cgaagcgggc catcatggcc tccggcgcca gcgtgttggt caggttcccg
    14761 cgcccgcctc cgctggccat cagccgcagc ccggggcgtt ccaactgctc gcagatccgg
    14821 acggaggcgc cgcgccgcgc cgcggagagc gccgccatca ggccggcggg gcccccgcca
    14881 acgacgatca cgtcccggcc tgattcctgg ctccggtgag tgggcatgcg gggcaggata
    14941 gccgccgctc ggccgggcgt gaagcctaat gccggcaggc gcgaatttcg atttggcgcg
    15001 gcgcctccgg aagagtataa agcaagcagc cgtgacggcg atgaaggtgc cctttccatg
    15061 gcggcgaaac ccggtcgggc cggcgggccg gcggggccgc gcgtggtgtc tcgcggccgg
    15121 ggcggcgctt gcgtggggct tggggcccgg cctctttgcg gattcccaat ccgacaccac
    15181 cgccttcctg caagggaaaa aggtgctggt gctgtgttcc taccactacg gcatgcgctg
    15241 gaccgattcc atctgcgcgg gcatccggtc ggtgttcgac gacccccagg gcaagtggca
    15301 cgtccttcaa tacgagttca tggacgccaa gcggatttcc tcgccggagt acttcgagga
    15361 acttgcgcgg ctcttcgcca tgaagttcaa gccccgggag ttcgacgtca tcatcgcctc
    15421 ggacgatgac gccctttctt tcctgctcca gtaccgggag cgcctgttcg aatccacgcc
    15481 ggtggtgttc tgcggagtga actacttcca gcccgagatg ctggcgaacc agtccgacat
    15541 cacgggcgtg gtggagtcgt tcgacctggt cgcgacgctg gatgccgccc tgatgctgca
    15601 tcccaacgcg aagctcgtgt acgtggtgaa cgaccagtcg tccaccggcc gggccaacaa
    15661 caggcggatc gaggacgtca tgccccggta tgccgagcgc gcgcatttcc gattcacggg
    15721 cccggcgagc atggcggaac tgctggagga gatccgcggg ctcccggaca acgcggtgat
    15781 cctgctcatg acgttcaacc gcgatcgcct gggggaagta ttccgctatc gcgacgcggc
    15841 ccgcttggtc acgggcgcca cgccgctccc ggtctacggg gtctgggcct tctacctggg
    15901 cgacggcatc gtgggcggca tgctgacccg gggggagacg cacggccggg cggccgccga
    15961 gatggccatg cgaatcctgg gaggcgagcg tgccgaatcc atcccggtca ttctgcaatg
    16021 cccgaatcag tacatgttcg attacaacca gttgcgcagg ctcggaatca acgtttccaa
    16081 gctccccccg ggaagcctgg tgatccacaa gccggagccc atgtaccgtt ttcgcaagca
    16141 gacggtatgg ttggtggtgt ccgcgttggc cgtcctcggt ctcctcgtcc tggcgcatgc
    16201 catcaacgtg aacaagcgtc tccaggccca gcacgccctg caggaggcca acgagaaggt
    16261 gcgccgcgag tcggccgacc gccgccaggc cgagcaggaa cgggagcgcc tggcggcggc
    16321 cgtcgcgcag atgtccgacg gggtcatcat ctcggacagc cagggcgtga tctgttatgt
    16381 caatccggcc ctggagcgga tgaccggtta ccaatccgcg gaactcctcg ggcaaagcgc
    16441 ccggatcttc ggccggccgg aggacgtccg gcaggtgcgg caggtgctgg aggcgctccg
    16501 gcacagcgag aactggcgcg gccggatgcg gacccggcgc aaggacggca cgctgcgcga
    16561 cgaggatgtg ctgatctgcc cggtgaagga ctccgccggc cgcgtcggcc attacctggc
    16621 catccgccgc gacgtgacgg aaagcgtggc cctgcagaac cagttgatgg aaacccagaa
    16681 gatcgaaacc gtcggcgaga tcgccggcgg catcgcccac gacttcaaca acatgctgac
    16741 cgtcatcctc acgagcgccc agttcctggc cgacggcgtg gccggcgacc cgaagttgag
    16801 aaaggacgcc ggggaaatcg tgcggaccgc caagcgcgct tccgaactgg cgcgggagct
    16861 gctgaccttc tgccggaagc aaccgctcga gctcaaggcc cgcaacgtga acgagatcgt
    16921 gttcgggctc gaagccatgt ttcatcacat cctgggcgaa cgggtccggc tggtgctgga
    16981 cctgacccgc gaagcctgca ttgcccaggt ggacaagggg cggctggagc aggcgctggc
    17041 caacctggtc atgaacgcgc gcgacgccat accggcccag ggcgtcgtga cgctgcggac
    17101 ggcgttggcg agggtggagg acgaaccggc cgccgaattt ctcgagccgc ccatggacgc
    17161 cgccgggcgt ttcgtccgga tctcggtgca ggacaccggg accggcatcc cggagaacat
    17221 ccggcagcgc atcttcgagc cgttgttcac gacgaaaccg gcggaacagg ggaccggcct
    17281 gggcctttcc gtggtctacg ggatcatcaa gcagcacggc ggacgcctgg ccctggccac
    17341 gcaggtggga aggggcagca cgttcaagat ctatctcccc gcggcctaca tcccgcctgc
    17401 caacctggcc gccaccggcg cgatcgcgcc cgtgcccagc ggccgggaga gcatcctcct
    17461 ggtcgaggac gaacgttcgg tgcgcgacgt catcgcgcgc atcatgaccc acctggggta
    17521 caaggtgacg gccgccgatt gcgcggaaag cgcgctcgaa ctgatggacc ggaccacggg
    17581 ccccctggac ctcctcttca ccgacatcgt catgccgggg atggatggcg tgagcctggc
    17641 ccgcgaggta ctccgtcgcc ggcccgggct gcgtatcgtc tttgcgtcgg gttattccca
    17701 ggaacacttg agcaaggatc cgaagttcgc ctgcatcccc ctgctgaaga agcctttcga
    17761 ggccggcgac ctggcgcggt tgatacgaag agtgctggac ggccccgaac aacgccccgg
    17821 tccggccgca tgatgtccgg gcaggagtgt tttccatcat gaaagaggcg ccgcggttcc
    17881 tcagggcggg atggatgctg tgctggctgc tgctgggcgc gacggcggcg cttcaggtcg
    17941 cggcctggaa ccacgcccgg gctttccttc gcttccagca ccggggggcg tcgccaacac
    18001 ccatcgagtc cttgggcgcc cgggacaagg tccgcctgct gttccgcggg gcggccaagc
    18061 cgcgtcccga gaaccggcgg gcgccggccg acgtcgggct gacggcggag acggtgcgct
    18121 tttccgcggc cgatgggacc ggactggagg cctggttcgt gaacgcgccc ggcgacaagc
    18181 cgccggtcct gttcttccac ggatacgcat cggcccggtc cagcctcctg gacgaggccg
    18241 ccatggccag ggacctgggc catcccgttt tgctcgtgga cttccgcggg agcggagggt
    18301 cggacggaaa cagcacgacc ctggggtacc tcgaggcgct ggacgtaaag gcggccgtgt
    18361 cgtggatcag gacccggctt ccgggccggc ccgcgtgctt cctttacggc cagtccatgg
    18421 gcgccgccgc cgtgctgcgc gcggtgacca tccacgatgt cgccgcgggc ggccttgttt
    18481 tggaggcggt gttcgatacc atgctgaacg ccgtgcgcaa ccggttcaag gccatgggct
    18541 ggccggcctt ccccgccgcc gagtcgctgg tgctgtgggg cggcgtgcag tgcggcttca
    18601 acggcttccg cctcaatccc gccgactatg cccggtcggt gaagattccg accctggtcc
    18661 tgtacgggga ccgcgacctc cgcctgaccc tcaagcaggc ccggcgcgtt ttcgaccggc
    18721 tggcgggccc ccgcctcatg gtggaattcc cacgggcgtc ccattcctcc tgcctggcct
    18781 ccgatccggc gcgctggcgg gacacgatga gccgcttcct ttcgggagag gccacgccct
    18841 gatccggtcc atctttctgc ttgtaacccc ggtcaaacgc tgtagattcg gaccatatga
    18901 accgcgcggg tattgtcctt gtcacgatga cgggaggctc ttgagagggc cggcggcgga
    18961 tacttttttc gttgtggccc cctcgcgtga gggggttttt cgtatcggtc aacgggatgg
    19021 atggacatga aagaaatcgg cataggtttg ctcggcttcg gcaccgtcgg cgccggggtg
    19081 gtggaggggc tccagcagaa cgggaaactg ctcgaggagc gtctggactt gcggctggcc
    19141 ctgcgccgac tggccgacct cgatctggac tcggaccgag gtgtgaaggc ggaccgggcc
    19201 ctgatgaccc gcgacgcggg cgaggcgatc cgcgatccga acgtggacat cgtcgtcgaa
    19261 ttgatcggcg gcgtgaccaa ggcgaaggaa ttcatccggc aggcgctcga actcggcaag
    19321 cccgtggtca cggctaacaa ggccctgttg gcggaggccg gcgccgaact tcaccggttg
    19381 gccgcggaga agaagaccgc gctctatttt gaggccagcg tgggcggcgg catcccgatc
    19441 atcaaggccg tccgcgaagg cctggtcgcg aaccggatca ccagcgtgta cggcattctc
    19501 aacggcacgt gcaactacat cctcacccgg atggaggagg aaaaactgcc gttcgagcag
    19561 gtgctcaaag ccgcgcagca ggcggggtac gccgaggccg aaccgagctt ggacgtggat
    19621 ggccatgaca cggcccacaa ggccgtggtc ctggcctcgt tggctcacgg ctttcccgtg
    19681 ccgatgtccg ccgtgcacgt cgagggcatt cgcaacattt ccgccgggga catcagctac
    19741 gccgccgaga tgggctatcg catcaagctg ctggccgtga tcaagcaggg cgggaacggc
    19801 gtcgaggcac gcgtgcaccc gaccctcgtg ccgcacgccc acccgctggc ggcggtcagc
    19861 ggagtgttca acgccgtgct ggtttccggc gacatcgtgg gcgatacgct ctactacggc
    19921 cgcggcgcgg gccgccgcgc gacggcgagc gccgtcctgg ccgacctggc cgacgcggcg
    19981 cgcgacctgt catccggcgc gcgccgcccg cccgtcctcc ggcgagagtc cggcaagctg
    20041 cggccgatcg gcgagatcgt cacccgctgc tacctgcgcc tgtcgctgct ggacaagccg
    20101 ggcgtgctcg cgctggtggc gcaggccctc ggccgcaaag gcatcagcat cgcctcggtg
    20161 atgcaaaagg agacccgcgt gggccagcac gtgcccgtgc tggtcgtgac ccattccgcg
    20221 ccggagagcg cgttccgggc cgccctggcc gagatcgaca ggctggaggt ggtcggcgcg
    20281 ccgaccgtga ggatccggat cgaggatttc caggaataga gggcgacggg cccaccgccc
    20341 tccccgagca aacggaatgg agtttgtcgc atcatgaaag tagccaaatt cggcgggacc
    20401 tcgctggcgg acgcggggca gatcctgaaa gtgctcgaga tcgtccgggc cgacccggat
    20461 cggcggatcg ttgtagtctc ggcgccgggc aagcggtcca aggacgacac gaaggtcacc
    20521 gacctgctga tcgcctgcgc gaaggcggcg ctggcgcgcg gcgaggcgga agcggaattg
    20581 gcgctcgtgg tgcatcgctt cgccgagatc ggccgcgaac tggacctgcc ggccgccctg
    20641 ctgacggaca tcgaacagga tttgcgcggc cggctggccg ccgaccgcag ccacaagggc
    20701 ctgttcacgg atgccgtcaa ggccgcgggc gaggactaca gcgcccgctg cgtcgccgcc
    20761 gccttcacgc ggaaaggcgt gccggccacc tacacctgtc cccgcgaggc cggcatggtc
    20821 ttgagcgacg aacccggcaa cgcccaggtg ctgccggaat cctacgataa gctggccgcc
    20881 ctgcgcgacc ggcccggcgt gacgatcttc cccggcttct tcggctacac gaaggccggc
    20941 cggatcgtca ccttcccgcg cggcggatcg gacatcaccg gcgcgatcct ggccgccgcc
    21001 gtcaaggcgg acgcgtacga gaatttcacc gacgtggatt cggtctattc cgcggacccg
    21061 cggatcgtgc cggacgcccg gccgatcgaa ctactgacct accgcgagat gcgcgagctg
    21121 gcctactcgg gattcggcgt gttccacgac gaggcgatcc tgcccgcggt gcacgcgaac
    21181 atcccgatca acatccgcaa caccaaccgg ccgtcggcgc cgggcacgat ggtcgtgccg
    21241 gagcggcccg cgcacactgg cggcgtggtg ggcatctcca gcagcgacgg gttctgcacg
    21301 ttgtttttga gcaagtattt gatgaatcgc gagatcggat tcggccggcg gctgctccag
    21361 atcctcgagg aggagggcct gtcctacgag cacgcgccct ccggcatcga caacacgagc
    21421 gtcatcctgc gcgatcacca gttctcgccg gagaaggaac gccacgtcgt cggccggatt
    21481 caaaaggagc tggccgcgga cgacgtcgag gtggagcgcg gcctggccct gctgatggtc
    21541 gtgggcgagg ggatgcgcta taccgtcggc ctggcttcgc gcgcgacgca ggccctggcc
    21601 ggggccggag tcaacatcga gatgatgaac cagggttcgt cggaaatcag catgatgttc
    21661 ggggtcaagg cggaggatcg caaaaaggcc atcaaggccc tgtaccacgc attcttcgcg
    21721 gcgtgagact ccgcccggcg cgggctattc catctccagc cggaagaact gctccgccgg
    21781 gccgcccggc gaaaagacga cattcgtgtc atacccgttg cccgccacgg gggggagggc
    21841 cgggatggct gcccagggac cggccagcgc cggggccgac tcggcctgat agctcttgcc
    21901 cggcagcgtg ctgaaatcca ctcggaccac gccggcggcg cgggccaggc tcacgattcc
    21961 gggcttgagc acgtcgtctt cgcgcgcgtt caggcgctgg atgcgcccgt cggccgaggc
    22021 ctccgtgtcc ggccggctgc actcgcccag ggcccagagg taatcggcca acgccttctg
    22081 ctcgccgccg tcggtggtga aggtcttgac cgcgtcggcc ggcaccaggt tggttctctg
    22141 tattccccgg gtcaacgcga agtagcggtc tccgccgtcg gccatgtact ccagcgtcac
    22201 cagccggaag gtccggttgg agtcgccgac gagcgctccg ttctcgacca ccaggtcgcg
    22261 ttgaccatcc gcccggcgcg cgaccagcga ccggagccgc tctccgggaa aaaggatgtt
    22321 ggtcggcgtc ccgtcttcgg tggtggagta ggtcatgggg gcgttcgtgg gattgaagct
    22381 gaactccagg ccgccgacct gcgggaactg tcccggcgtg ccggatcccg cgacgcccca
    22441 ctcgacggcg tcgcgcaggt cctgggccgt gatcgtcagg atactcaagc tgttgttgaa
    22501 gcgcagcgcg ttctcgatgt ccaactgcga gacgtcgccg tcctgcttgc ccacgcgcgg
    22561 attagcgagc ggcgggaccc ggatcgaacc gccgccctcg gacaggatgg cgccgatgga
    22621 atcgcggatg ccgccgccgt tcttcagcga gaggctggcc gccggatccg tcagcccggc
    22681 ccggtagaga ttggcgtcgg cggacaagtc gcccaggttg gtttcctcgg tccgcacgct
    22741 ttcgcggatg ccgttgaggt acaccgccgt gcggccgaac aggttgccgt ccttggcgtc
    22801 gatgatcccg gccaggaccg ccaccacgcc ggtgacggcc ggggccggcg ggtatccgcc
    22861 ggtgtcggcg acgccctggt cgtcggtcgc gtacgcgccg ctcgcggtgg aaacgttggt
    22921 gatgaagccg cccgcgtcga aatcggccac gaagcgcccg acgtaccggt aattggcggc
    22981 cgtgttcagc acgtaaacca cccggcccac gaggtcggtg cggacgaggg gatagtcgcc
    23041 gtaccgcgta tcgccggccc gcaaccggtc gccgggcttg gccagcaggg tgtgggagcc
    23101 gccggcgatg aggatgtcca cgccggccag gcgcccggcc aggtcgagct cgttttccag
    23161 ttgctgcagg tgagccagca ggatgatctt gttcagcccc gcgttggtca gcgcatggac
    23221 ggcggcctgc gcctcggcgg ccacgttggt cagcacgatc acgccgccgg ggctggacag
    23281 ggtcgccagc tcgggcgtgg ttacgccgac cactcccacc gtgtcgccgt ggacagcgat
    23341 ggtgcaactc cgcgcgatca ggttggtcac gagcggatag ggctggccgt cggcggcgac
    23401 ctgcgttttc aagttgctgt cgttggtgaa aacaaggttg gcggccaggt aggggaaggc
    23461 cgtgccggga tagttgacga ccgcgtccgg
//
LOCUS       DAOOTK010000038        23310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1518_length_23310_cov_6.590411, whole genome shotgun sequence.
ACCESSION   DAOOTK010000038 DAOOTK010000000
VERSION     DAOOTK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23310
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_23310_cov_6.590411"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PK634_06315"
     CDS             <1..1504
                     /locus_tag="PK634_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97386.1"
                     /translation="LSWPDRQAWEQAYERGVAAFRSRDWAAALGHFDAASAKDDAYAE
                     LAYRRAVCLQQLGRLAEAEPVFARALDLDAFRYRVDSRLNDAIRTLGASLPGEVELVD
                     VARAFRNNGASRDTEWFVDHVHFSFEGTYELARLWADALARVPPFRDLSPAANGEWSL
                     EEAKAALLFTPVAELGFVSALLGRYGQPPFNQQFDVNERIAAYNRRLGALTLQIQAMD
                     VEQADARFAAHEERNPGDLAAPYLRGQLLLSFNRYGKAAEALDSVLRRQPHRRDLRSL
                     LARALAAQGRADEAAEALVGWTRRHGFLAAQAAVGHMNELVQNGQEDEALAYGRAVAA
                     RLRAADYRWRVRREIGGLEYLTTRRDEALEAMRRGDPDTAAARWSDLMQRRPDWPVAP
                     YWLSVMRSGRGDREEGLALIQRALNSWGYARASYHLGLWEARFGDPGKAKELFRDAAR
                     AAGEDARLAASLAWVLTAHPNPDVRDPALAQERLAPAEPPPAGFVPMNFF"
     gene            complement(1608..2633)
                     /locus_tag="PK634_06320"
     CDS             complement(1608..2633)
                     /locus_tag="PK634_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOW97387.1"
                     /translation="MAIADGWYHVTSRGIDRRAIFHDDHDRRHFLDLLGALPERFGIR
                     IHAYVLMTNHYHVLVQTPQGNLSQGIHWLNCSYGIWSNCRHRRVGPLYQGRFKAVVVD
                     GEGSWALDLGRYIHLNPIRTKRMNLGKKEQKVERMGWALPDKTEAESRVEALRAYRWS
                     SYRAYAGYASLPEWLTSDTLMGRLSEAKRKSPATYRRWVEEGVLDGKEESPWLKMKGG
                     LALGSERFLSVLRKMVKGDRREQTPLRKLESDRTFDQVVKAVETAKGEKWETFRDRRG
                     DEGRDLALWLARKHCRMTLKALGDKAGGMDYLAVSKAVSRMAERLNKDRTIRQILAKA
                     ESDMSNV"
     gene            2897..3175
                     /locus_tag="PK634_06325"
     CDS             2897..3175
                     /locus_tag="PK634_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97388.1"
                     /translation="MNSLAQVALGCLNQHMIMIGHQHIGVNSNPEPFRQGAQQVKKMS
                     PVMIVVKYRPPINAPGRHMIPAIRYHHSKGSRHARTLPVFLSLVNPYV"
     gene            complement(3747..8222)
                     /locus_tag="PK634_06330"
     CDS             complement(3747..8222)
                     /locus_tag="PK634_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOW97389.1"
                     /translation="MFLDGTKHHFTKQTDGGTKLFRMEATIDPFSNRYSYAYSNGLLS
                     QVTGPNTNQYLRFSYQPATNSVPAGSIEFYYIETNASEVLLAGSFNGWSAQPMTQTSG
                     VWQRVETLARGWHYYKFIVRYAGDTNDYWRIDPDNPTWAYTGSASDLQTNSVAVVDVY
                     LISSVLASDGRCVTYQYGNWMPSLEAVDIPLVRADYGDGTYAEYSYYQPADDLYRKAL
                     MKTARDPHYEGPGRAIWYVYQTNLAFTGEIYEERSMVNSQMLSRLLLDNNNADKRIVV
                     DADGHQTVQDFGTNAYATNAVGYVTRGIHHAVGGDVGMLWKWIDAMGRTNVYERTVHF
                     GRATSVWNNVRGTRVFRYTDDMYPFYLAERMDEAGRTTKYQRDDKHRVVRVDYPDGSF
                     ETNSYNDSGQMVCSLRRNGAADSYEYDERGRNTCLIDGLGNRTCYAYDDCDRLAAISN
                     SLGYVTFFSYNWRGQVTNAVFPDGTQERLWYDEYGVLTGRTDRAGGMRILARDAFGFL
                     NAETDPTGATTYYQHDLSGRLLKTITPLALIVSNTYDGIGRKTREIYADDGTFREWHY
                     DPDGVRTQYDRLAGPPTTFKYDPHGRLQCVTDPLNRTVSYGYDAAGNCTHETNALGEA
                     IVHTFDLAGRVTSTRDCNEHTVSNVYDETGRLVQQIDANSNANIYTYDALGRLLCLTR
                     GGPGFTPALLQTNSYNALGWIITSTDANGLMRSNTYDVIGRPLRMYMPDGAYNENQYS
                     NTFLVKTLDRAGRQTVLQRDILGRVTNQVDNAGQSVRFVYEALGNLSDLYDQNGARTR
                     FQYNAEGRQTAKIYADDTQYTYGYDAEGRLAIKLDAKNKTTVYQYDYVGNLTNIVYAA
                     DPAVSFTFDALNRMTRMVDGVGTTLYAYSGSCGALVSEDGPFENDALSYEYDNGKRLL
                     SVTSVLSAVFYSYDALDRISAVVGRVGPNAPLTNTYTYHANGRLPSDLLRGNGTRTRY
                     EYDLLKRLTNLVHETDASEVLASYAYTLNNADERTRVAIGAGTPSSRTIDYAYDPIGQ
                     LIGADSDQPGHSFAYSYDPAGNPVQQDNNGFVLTNVFNDLNQNVTSLWSGAVAVLGTA
                     NLTNGQISVQGGPASTRADGLGGLIYAATNLPVSDGTNTYTALLSDPFGRVSTSAVSV
                     IARNRGYGYDANGNMTNDGQLAYTWDDADRLIEVGRVGSNAPPLMTCRYDGLGRRRER
                     ILSAVAGGGDPGSTNRYAYNRWLVVAVLDGSDLVLETYTHGPDLSGTLGGAGGIGGIL
                     TDFRPLTSDLRHFHFDGNGNVIACTDTNQTIVASLEYGPFGTLLTRSGSFTPRYRFSS
                     KEWDDPVGLYCYTYRFYSPALGRWLSRDPVGEHGGINVFTFTMNSPHSKLDPFGLCET
                     CGTGPGTGYRCCGGLPYSIINEGCCDDKDRYGKEYECCENKKVYDRKPRKDCPTATGF
                     CACNGGVWGQPSTWASAICSAIGGAYGGTPGSIIGGFVGIFIGEGYCNGKVCCVE"
     gene            complement(8213..8791)
                     /locus_tag="PK634_06335"
     CDS             complement(8213..8791)
                     /locus_tag="PK634_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97390.1"
                     /translation="MKKALTGILLYAVVAGSPRCSYAASSCGNPPQPPDVCGVCGGTT
                     TDPDCCNQNNGGGGNSPAAPGSGWGGAANGPSCSSCGSGANPFNPFSGSVGREIKDLE
                     LAHTVGAFKLQMTRHFASRWDAYPHIAFDTPFGGAGYWRHSWQWSILDFGTNEIGQER
                     IRIIYPNGKFGNFAKDAVSAFFPMAPITTSCF"
     gene            complement(8788..9288)
                     /locus_tag="PK634_06340"
     CDS             complement(8788..9288)
                     /locus_tag="PK634_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97391.1"
                     /translation="MKKKRIWVSLAMYACCAFVSTAGYRAVDEPQRRVASGSAADTYS
                     RSSKSIEPAGGWTEISVTRTNHEIQLGNHAGVTDQLTLNPDEEVVVVARKPHYPKGIS
                     VFLYTLHGGLVNGKPTDSMTTDGTGTISFRFQIGPWSGNYPLILRSGGREEEISFWVT
                     KKDEKR"
     gene            complement(9285..9938)
                     /locus_tag="PK634_06345"
     CDS             complement(9285..9938)
                     /locus_tag="PK634_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOW97392.1"
                     /translation="MNVPFMPTAGRTAFGLLFALCLHARAEGPAWWTTRGVLDTNAPA
                     GDYAPVNQGQLKHIAVQACSELDDSIAQFGGAGTAISNLVAGFSNTTNYAPVNIGQLK
                     NTALPFCERLMAIGYTNDYPWAGASATNDYAAANIGQMKYLFSFDLVLDTDEDGMPDW
                     WELANGFDPEGPSDAEEDPDADGYSNQEEYQAGTDPAGFNPSPVVAITYPRDGEVLP"
     gene            complement(9962..10963)
                     /locus_tag="PK634_06350"
     CDS             complement(9962..10963)
                     /locus_tag="PK634_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97393.1"
                     /translation="MTRRLLCVWMVLVACCFAVLAQMPQQFVYVGETTNGPTIQLLLR
                     LYTNDVGGVCLFEDSNTVQVADGYYSALVGDTPTTGTLSEALGCGEVYIEPVVNGAPE
                     ARELLHPNAYALVADGVLQGKITSAMIATGAIQQAHIQSGAIQSAHISEGAVSYWQIQ
                     NGTVGTNDLNVGELDGRFVNVTGDTLAGDINLGGKKIGGVATITFANNPPYISGPVLS
                     NYYTSIYINNGRILTSADNIDHLYSFYSLGGGWAGNYGLSWLKLRSVTSAGATWTTNY
                     TASFYVTSNVVNEIRVTGHFNMQGSAISNGVFVGNGSALYVLPQGGLSMGTFTNGLP"
     gene            complement(11268..13214)
                     /locus_tag="PK634_06355"
     CDS             complement(11268..13214)
                     /locus_tag="PK634_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97394.1"
                     /translation="MLRRLILRNFLPGSITTDDPSGLESPGQSMHKRYPACLVMGLLL
                     LGLAAHLGADVWPRDAFSWMDPEQYYHWAGRIASGGATLRGFPVASIYPLLLAPALAV
                     CGSIGFALATNAIWLFFMAWGVYSLCRHIPLTIPAPVVLATMVSSPLLLGLSRELYLE
                     FGLTALVTLTYAAWFARRRPPGRTGFDFFPVLFGLGFALKMTWPVFFVGPVLATLVRA
                     IRTRDGRQCLWLAGAVLAPPAVVVGLFWLLNPAGFAYYMSAGNTALRSMRLLGPPDVI
                     SSASALYYPAAFGRYGLFLLAPFFLWLAVRASLPDRAVSKTDREIVGDLWLWLIVPIA
                     LLTLQVVKEPRHIAPCVVPAVLLLFKGLDRLRARATRVIVSGVVLTLAATQYALTASR
                     AVYAPYYLNRSLNIEALKQVLFENMPGREPYVDEAGVIDVPRWLFTRSLLISGFPPNE
                     SLALVWAFRPGIVMDMENFTRPHIEYATGYEEFMELFSLAGANAYNRRCGWNGCYRTL
                     DRDQAARGADALILFSAPGIKPSMKRDGFSCATTVPLADGSAMSVYLPARAPSESFRQ
                     TYAKTFLKENPGASDLELNTVYYALLLQQYLRTGSTALDISSAGLPAGFVPGPERRNI
                     YFISMYTPLARRLDEALARAQKQP"
     gene            13756..15459
                     /locus_tag="PK634_06360"
     CDS             13756..15459
                     /locus_tag="PK634_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97395.1"
                     /translation="MEIVAQSGMKSSFGAALGIGLSIILIYGACLAFFIPRDGFWAAD
                     TGCKFIQMEGLIQSGYRVFDIRWPGAAVDPDLAWAPIPYPFGIKIGGKLFLQYTPIFA
                     LLASVPYRLIGFAGLYVLPCLAGLLLLPAVYGIARETGSRPGAAPLAVLLTGLAGPLW
                     FYSLSFWEHTLAVALLGWSIWAGLCHLRTGTALPLVVAALLSGLAIYVRDELYLFAGI
                     WAVVQGFLSPRRRIAWLFPAVAVVTLIPLWIFYYCSLGNPCGYHFVQAKEAFNLARFV
                     SERVVVFRNLFLNAHPQPIVSLLLSIPLLSLGLFRSLDDRWAPGIFLLMGLSGLLILV
                     TLAASTSPMDTMLYTNGLFAPAPILAVGFARFAPAKCASVMSGSRGLKALVWLTVGYA
                     IGYGCLAPVVSSQGIHWGCRFLLPVYPLLAILAARVITALWRNSLAGKATITAAVGVA
                     VLLQLFSIRLLIQRQTYTKNVASVLSVRPERVFVTNVPLLPTTSLAPLFFSRPIFLVR
                     TPEELTTLLARLREAGVQDVAWLTHEPPHSVPAPGAIRIHDPWRRFLAVDVRTIRVAS
                     P"
     gene            complement(15462..17171)
                     /locus_tag="PK634_06365"
     CDS             complement(15462..17171)
                     /locus_tag="PK634_06365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW97396.1"
                     /translation="MRPDALIQSSAGKSRAVTVLFLAAGVLLHLALGGAAIRAQSAVF
                     DEPPHLLSGYIFLATGKDCFNGLYHPPVQQLMAALPLLGLKPEVPREYVAAPYRDPNP
                     AYALAHRFLFHNGAGTDPDRLLNTGRAGVLVFSGLAAVAIWGVCARFFNPRAAWIAFF
                     LYILNPAVLAAASLVTTDLAVSAFFFLFFLCWGRWEQRGQRRDAVWAGTLLALALASK
                     FTAIAALPVLALWFIARKGRLPFPWSQVGWFAAALILMLALIYRGHELPVFVEGLRGV
                     LFMTKKGQPTFLFGRHSETGWWYYFPALFLIKTPLPLLLGLLAAASAVLRKPMRIPWY
                     LWLPPLVYFVLLCRSDIQIGHRHLLPVYPFLFLAVGLFLADRLPRRPWAIASAALGLW
                     YAAGTAYSAPYFLAYFNETVGGPANGYKLATDSNVDWGQGLRALRDYVLKENISGFYL
                     SYFGTADPNAYGLEHVSVGSVTNPDFHDRDPDLRKERRALLVVSATNYQSTYYEGHRN
                     FEWLHARTPVAVLARSLLVFDITRDADAHRQLAAYFDAVGRSAMAAIERDWAAALDAS
                     PFF"
     gene            complement(17231..20140)
                     /locus_tag="PK634_06370"
     CDS             complement(17231..20140)
                     /locus_tag="PK634_06370"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOW97397.1"
                     /translation="MLDTRESVELCDKDAAHSRRGPAAKSARRAAPAAKKKARPGLAI
                     PRVFSTTGVNPFDAVEWERRKAVIGDEKGKTIFEQDNVMVPRAWSMLATNVVASKYFY
                     GELGTAAREHSARQLIHRVARTIADWGLADGVFASPEDAEIFYNELAWLCLHQHGSFN
                     SPVWFNCGLHHQYGTGKGNGHGLLYFDRAEGRVRRAETQYEKPQCSACFIQSVEDSME
                     GIMRLAHSEAMLFKFGSGTGSDLSTLRSEREKLSGGGRPSGPLSFLKVYDSIASVVKS
                     GGKTRRAAKMNTLKCWHPDVKEFIQAKQNEEKKAWALIEQGYDGNFNGEAYASIGYQN
                     ENLSVRLSDAFMRAVLEDGEWPTRWVTDPAKSGPTYKARDLMRWIAEGTWTCGDPGVQ
                     YEDSIQRWHTCARTGPINSSNPCSEYMFLDDSSCNLASLNLMKFVRDDGGFDVERFRA
                     AVRIFVTAQEIIVDNASYPTESITLNSHRFRPLGLGYANLGSLLMALGLPYDSDRGRA
                     IAGALTAVMHFGAYTQSARLAEALDPFEGFAENRETMLEVVGRHRAALDTLPPGCPPD
                     LLAAARAAADEALEAGKKHGYRNAQVTVLAPTGTIGFMMDCDTTGVEPDIAIVKYKLL
                     AGGGMLKIVNRTVPAALQRLGYGRPQVQEILEYIEHNDTIEGAPGIKDEDLPVFDCAF
                     KPRNGRRYIPYGAHLRMMSAVQPFLSGAISKTVNMPTEATVDEIMQTYIDGWKLGLKA
                     IAIYRDGCKRSQPVNTGKTEKAAEKPGGNGSKARPLRKRMPSTRQSITHKFDIAGHEG
                     YLTVGLYDDGSPGELFITMAKEGSTIGGLMDSFGTAISMCLQYGVPLKTLVEKFAHSR
                     FEPSGFTKNPDIPIAKSLPDYIFRWLGHTFLEGFKAPAPAKSLQPEPAVAVPATDKPA
                     KPAAAPAHGMNVQVLDAQFKNFMEDAPVCDQCGAITVRNGACYRCYNCGNSMGCS"
     gene            complement(20356..21708)
                     /gene="der"
                     /locus_tag="PK634_06375"
     CDS             complement(20356..21708)
                     /gene="der"
                     /locus_tag="PK634_06375"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOW97398.1"
                     /translation="MSEPVPQRVVAIVGRPNVGKSALFNRLAGRRIAIVHEEPGVTRD
                     RLECRAEWAGRRFELTDTGGLSTMDASAAKDLFEAETQRQAELAIREAAALILVVDIT
                     AGVLPLDREVARRLHASGRRTVIAANKADHPGLDDQVSEFESLGFPVFPVSALHNRGL
                     DALMAPILAALPPPADIAEEPPLKVAIVGRPNVGKSSYINKLLGSDRVIVSDVPGTTR
                     DSIDVPFSVGAGESARRYLLIDTAGARKLGRAHTAVERFSLFRAQKSIERADVAVLML
                     DAVEGPGEQDKKLAAQILEHRKGCLLLVNKWDLARGVKEDEYEAALRRVLPFLDFAPI
                     LFVSSLTGYSLRRSIEAIDRVAEQTRRKLTTGLLNRVLHDAVARVQAPHIRGRRMKYY
                     YATQTETAPVTIRLFVNDPKRMTPAYRAYLVRELRNAFGLEGAPIVLQLKASHTGDHP
                     "
     gene            complement(21705..22340)
                     /locus_tag="PK634_06380"
     CDS             complement(21705..22340)
                     /locus_tag="PK634_06380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOW97399.1"
                     /translation="MTADHQTSHHMTLANRVTILRILAVPVFILMLLYYTQGLALGRA
                     EDGYRIAALVVFIVAAATDALDGYLARSRDEITHLGKILDPLADKALLLSALILLTRP
                     ALPSLEPHIPVWFTLLVISRDVVLILGAAVIHAVAGSVVVKPRVVGKVATFFQMITIT
                     WVLMQGRPRLFAVWILLAGSFTFISGVWYLFDGLRQLEKHSAAPGRMPSPP"
     gene            22371..22832
                     /locus_tag="PK634_06385"
     CDS             22371..22832
                     /locus_tag="PK634_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOW97400.1"
                     /translation="MPGSRKARFFAAPAGFRLAPAADRGRISQRKRMTMHWLLLMVVG
                     LAVGALSGLLGIGGGVIVVPVLVMGFGFSQHTAQGTSMALFALPIGLLAAITYYRHGH
                     VNLPAAGLICLGFVIGGLLGARLAVILPELLLRRVFGIFLLVISVRLIFFK"
     gene            22845..>23310
                     /locus_tag="PK634_06390"
     CDS             22845..>23310
                     /locus_tag="PK634_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97401.1"
                     /translation="MASVSVREGVCHALYVFDIGLSIRLDRVRGLLARSDVKTEATTS
                     RRAPKYYGGYQPAPLHVAQRAEALQVADRALSSAVDMVLYDFGAVTVAYAIPFEGPLS
                     ELGRLSCELNAGDALERDARGRVDGLLRRIRRAVSRPRPTGPVEDYLMFEVRQ"
BASE COUNT         4324 a         7081 c         7244 g         4661 t
ORIGIN      
        1 cctctcgtgg cccgaccgcc aggcctggga gcaggcctac gagcggggtg tggccgcctt
       61 ccggtcccgg gactgggcgg ccgccctggg tcattttgat gccgcctcgg cgaaagacga
      121 cgcctatgcg gaactcgcct atcggcgggc tgtttgcctg cagcaactgg gccggctggc
      181 agaggccgag cccgtcttcg cccgggccct ggacctggat gcctttcgct atcgcgtcga
      241 ttcgcggctg aacgacgcga tacggacgtt gggcgcctcg cttcccggcg aggtggaatt
      301 ggtggatgtc gccagggcgt tccggaacaa cggggcgtcc cgcgacacgg aatggttcgt
      361 ggatcatgtg catttttcct tcgagggcac ctatgagctg gcgcggttgt gggcggacgc
      421 gctggcccgt gtcccgccct tccgggatct ttccccggcg gcgaacggcg agtggtcctt
      481 ggaagaggcc aaggccgccc tgctgttcac gcccgtggcg gaactcggct tcgtgtccgc
      541 gctcctgggc cgctacggcc agccgccgtt caaccagcaa ttcgatgtca acgagcggat
      601 cgccgcctac aaccgccgcc tcggggcgtt gaccctccaa atccaagcga tggacgtgga
      661 gcaggccgac gcccgattcg ccgcgcatga agaacggaat cccggcgatc ttgccgcgcc
      721 ttatctccgg gggcagcttt tgctttcctt caaccggtac ggcaaggccg cggaagccct
      781 cgattcggtc ctgcgccgcc agccccaccg ccgcgacctg cgcagcctgt tggcccgcgc
      841 gctcgcggcc cagggtcggg cggacgaggc cgcggaggcg ctggtcggtt ggacgcgccg
      901 gcacggattc ctggccgccc aggcggcggt cggccacatg aatgaactgg tgcagaacgg
      961 ccaggaagac gaggcgctgg cctatggccg ggccgtggcc gcgcgcctgc gggccgcgga
     1021 ctaccggtgg cgggtccgcc gtgaaatcgg cggactggaa tatctgacca cccgccgtga
     1081 cgaagccctg gaagcgatga gaaggggcga cccggacacc gcggcagccc gctggtccga
     1141 cctgatgcag cggcggcctg actggccggt cgcgccgtat tggctgtccg tcatgcgaag
     1201 cgggcgcggg gaccgggagg aagggctggc gctgatccag cgagccttga actcgtgggg
     1261 ctacgcgcgc gcgagttacc accttggtct ctgggaggcc cgctttgggg atccgggcaa
     1321 agcgaaggag cttttccggg atgcggctcg ggcggccggc gaggatgcgc gcctggcggc
     1381 cagcctggcc tgggttctga cggcgcatcc gaaccctgac gtccgcgatc ccgccttggc
     1441 gcaggaacgg cttgctcccg ccgagccgcc gcccgccggc ttcgtcccga tgaatttctt
     1501 ttgatgggcg cgatcacatg gaaaacggca tgcacagggc ggccaatccc ggggcgatcg
     1561 cgtgggcggc cgggctgctg gtcgcgcgag gaagccgggg gccgcgtcta gacattagac
     1621 atatcgcttt ctgctttagc cagaatttgc cggatggtcc ggtccttgtt caggcgctcc
     1681 gccatgcgcg acacggcttt gcttacggcc agataatcca tcccgccagc cttgtctccc
     1741 agggccttca gggtcatccg gcagtgcttg cgagccaacc ataacgccag atcgcgaccc
     1801 tcatcgcccc ggcgatcccg aaaggtttcc catttctcac cctttgccgt ttcaactgcc
     1861 ttaacaacct ggtcaaaggt ccgatcgctc tccagcttcc ttaatggggt ttgttccctc
     1921 cggtctcctt taaccatctt gcgcaggacg gacaggaatc gctcgctccc aagcgccagt
     1981 ccccctttca tcttcagcca agggctttct tccttcccat ccaatactcc ttcttccacc
     2041 cagcgccggt aggtggccgg acttttccgc ttcgcttccg atagccgtcc catcagtgtg
     2101 tccgaggtta accactcggg gagcgacgca tatccggcat acgcccgata cgaactccaa
     2161 cgatacgcgc gcaaagcctc tactctggac tccgcctccg tcttgtccgg caaagcccac
     2221 cccattcgct caactttctg ttccttcttg cccagattca ttcgtttggt tcgaatcgga
     2281 ttcaggtgaa tataccggcc gagatcaaga gcccagcttc cctccccatc caccaccacc
     2341 gccttaaatc gtccttgata caaagggccc actcgccgat gccggcagtt ggaccagatg
     2401 ccatagctgc aattcaacca atgaattccc tggctcaagt tgccctgggg tgtttgaacc
     2461 agcacatgat aatgattggt catcaacaca taggcgtgaa ttcgaatccc gaaccgttcc
     2521 ggcagggcgc ccagcaagtc aagaaaatgt cgccggtcat gatcgtcgtg aaatatcgcc
     2581 cgccgatcaa tgccccggga cgtcacatga taccaaccat ccgctatcgc cactcgaagg
     2641 ggcctcgcca tgcccgcaca ctgcccgttt ccccgcccct cgtcaatcct aatgtctaat
     2701 gtctagactc gaccccacct gcccgtagac tcgaccccgc ctgcccgcga atcggattca
     2761 ggtgaatata ccggctgata tcaagagccc agcttccctc cccatccacc accaccgcct
     2821 taaatcgtcc ttgatacaaa gggcccactc gccgatgccg gcagttggac cagatgccat
     2881 agctgcaatt caaccaatga attccctggc tcaagttgcc ctggggtgtt tgaaccagca
     2941 catgataatg attggtcatc aacacatagg cgtgaattcg aatcccgaac cgttccggca
     3001 gggcgcccag caagtcaaga aaatgtcgcc ggtcatgatc gtcgtgaaat atcgcccgcc
     3061 gatcaatgcc ccgggacgtc acatgatacc agccatccgc tatcaccact cgaaggggtc
     3121 tcgccatgcc cgcacactgc ccgttttcct ctccctcgtc aatccttatg tctaatgtct
     3181 agactcgacc ccgcccgccc cgacgaccag actagcccca gccagcttca agtcaagctc
     3241 gatatttgat atattaggtc tgacccgcaa cgctacagaa cgtgcgaata ctgttgcgcc
     3301 acgcaggaag gcctcggagc acagcggccg ctttggccaa ctcgctatta ccgacatcat
     3361 tccttcttcc gttgtcccat ggccagcgct ctcggacccg ttaccactcc gtagagttgc
     3421 cgaacattat cttctcggac aattgaaccc tcatgattct ttgggcaaca ggccgtctag
     3481 actcgacccc gccgcccccc tttcatatgc ataagtcgtt caggctctaa aacaacctgt
     3541 ggtccgaccc cggacgcctc gtctttgaac tgcccactcc tttgagggct acaagcatga
     3601 tcgtaaaggc gacaaattga ataagcactt gtgctttctc gctgtaatgc tgggacgacg
     3661 attctctggc gatggcaccg aataatacag atgcaagaat gcagaacaat gtgtggacca
     3721 gtgaagccat cttatgaacg ttggttctat tcgacacaac acaccttccc attacagtaa
     3781 ccctccccaa tgaatatacc gacaaagcct ccaataatgc tgccaggagt gccaccatac
     3841 gcgccgccta tagctgaaca tattgcactt gcccaggttg agggttgacc ccaaacgcct
     3901 ccgttgcatg cacaaaaacc agttgctgtt gggcaatctt tcctaggttt gcgatcataa
     3961 accttcttat tctcgcaaca ctcatattcc ttgccatatc tatctttatc gtcgcagcaa
     4021 ccttcgttta taattgagta aggtaggccc ccacaacatc tgtagcctgt tcctggtcct
     4081 gttccgcagg tctcgcacaa accgaaagga tctaatttgc tgtgagggct gttcatagta
     4141 aaagtaaata cgtttatgcc cccatgctct ccgactggat cgcgggagag ccaccgcccg
     4201 agagcgggcg agtaaaatct gtaggtgtag caatacagcc caacaggatc atcccattcc
     4261 ttcgaggaga accgatagcg aggggtgaag gaaccggagc gcgtcaggag cgtgccgaac
     4321 gggccgtatt cgagggaggc gacgatggtc tggttggtat cggtgcaggc gatgacgttg
     4381 ccgttgccgt cgaagtggaa gtgccggagg tcggaggtca gaggtcggaa gtcggtcagg
     4441 attccgccga tgccgccggc gccgccgagg gtgccggaga gatcggggcc gtgggtgtag
     4501 gtttcaagga ccaggtccga gccgtcgagg acggcaacga cgagccagcg gttgtacgca
     4561 tagcggttgg ttgagccggg gtcgccgccc ccggctacag cagacaggat gcgttcgcgg
     4621 cggcggccga ggccgtcgta gcggcaggtc atcaggggcg gcgcgttgga gccaacgcgc
     4681 cctacctcga tcagacgatc ggcgtcgtcc catgtgtacg ctaattgccc gtcgttggtc
     4741 atgttcccat tggcatcata gccatagcct cggttccggg caatcacgga aacggcgctg
     4801 gtggacacgc ggccgaaggg gtcggacagg agggcggtgt aggtgtttgt gccgtcagag
     4861 acgggaagat tggtggcggc gtagatgagg ccgccaagac catcggcccg cgtggaagcg
     4921 ggccctccct gaacggaaat ctggccgttg gtgaggttgg ccgtgccgag gacggcgacg
     4981 gcgccggacc agaggctggt gacgttctgg ttgaggtcat taaagacatt ggtcaacacg
     5041 aagccgttat tgtcctgctg gaccgggttt ccggcgggat cgtaggaata ggcgaaggaa
     5101 tggccgggtt gatcggagtc ggcgccgatg agctggccga tggggtcgta ggcgtaatca
     5161 atcgtgcggg acgagggcgt cccggctcca atggctaccc tggttcgctc atcggcattg
     5221 ttcagcgtgt aagcatagga ggccaggacc tcgctggcgt cggtctcatg gaccaggttc
     5281 gtcaggcgtt tgaggagatc gtattcgtac cgggtgcggg tgccgttgcc gcgaaggagg
     5341 tcggagggca agcggccgtt ggcatggtac gtgtaggtgt tggttagcgg cgcgttgggg
     5401 ccaacgcgcc ctaccacggc ggaaatgcgg tcgagggcgt cataggaata gaaaaccgcg
     5461 gataacacgg atgtcacgga taagaggcgc tttccgttgt cgtactcgta ggacaaggca
     5521 tcgttttcaa atgggccgtc ttcggaaacc agtgccccgc aactgccgga gtaggcatag
     5581 agggtcgtgc caacgccgtc taccatgcga gtcattcggt tgagggcgtc aaaggtgaaa
     5641 gagaccgcgg gatcggcggc atagacgatg ttggtcaggt tcccgacgta atcgtactga
     5701 taaacggtag ttttgttttt ggcatcgagc ttgatcgcca accggccttc ggcatcatat
     5761 ccataggtgt actgggtatc gtcggcatag attttggcgg tctggcggcc ctcggcgttg
     5821 tactggaacc gggtgcgggc tccgttctgg tcgtacaggt ctgacaggtt acccagggcc
     5881 tcatacacga atcgaacgct ttgccccgcg ttatccacct ggttggtgac gcgcccgaga
     5941 atgtcccgct ggagcacggt ttgtcgtccg gcccgatcaa gggttttgac gaggaacgta
     6001 ttggagtact ggttttcgtt gtacgcgccg tcgggcatgt acatgcgcag cgggcgtcca
     6061 attacatcat acgtgttgga gcgcatcagg ccgttggcgt ccgtggaggt aatgatccat
     6121 ccaagggcgt tatacgaatt ggtttggagc aaggccgggg tgaaacccgg ccctcccctt
     6181 gtgaggcaga ggagccggcc cagagcatcg taggtgtata tgttggcgtt tgaattggcg
     6241 tcaatttgtt gaaccagtct tccggtctcg tcgtagacgt tactgaccgt gtgctcattg
     6301 caatcgcgcg tggacgtaac ccgaccggct aggtcaaagg tgtggacgat ggcttcaccc
     6361 aaggcgttgg tttcgtgcgt acagttgccg gctgcgtcgt aaccgtagga aactgttctg
     6421 ttcaggggat ccgtaacgca ctgaagacga ccatgtggat cgtatttgaa cgtggtcgga
     6481 ggccccgcca ggcggtcata ttgcgtccgc acgccatcgg gatcatagtg ccactcgcga
     6541 aatgttccgt catccgcgta gatttctcgt gttttccggc ctatgccgtc gtaggtattg
     6601 ctaacgatca gcgccaaagg cgtaattgtc ttaagcagac gtcccgacag atcgtgctga
     6661 taataggtag tagctccggt cggatccgtt tcagcgttaa gaaatccgaa ggcgtcgcgg
     6721 gccagtattc tcatgccacc tgcgcggtcc gtgcggcccg taagcacccc gtactcgtca
     6781 taccacaggc gctcctgcgt tccgtcaggg aagactgcgt tcgttacctg cccccgccaa
     6841 ttgtaggaaa aaaaggtcac atatcccaac gaattgctga tggccgcgag acgatcgcag
     6901 tcgtcgtatg cgtaacacgt cctgttgccc aggccatcaa tcaagcatgt gttccgaccg
     6961 cgttcgtcat attcatatga gtccgcggcg ccattcctcc ttaaagaaca aaccatctgt
     7021 ccggaatcat tgtacgagtt cgtttcgaag ctcccatcgg gatagtctac cctgaccacc
     7081 cgatgcttgt cgtctcgttg atactttgta gtgcggcccg cctcatccat cctttcggcg
     7141 agatagaagg ggtacatatc gtccgtatag cgaaacaccc tggtgcctcg aacgttgttc
     7201 cagacggacg tcgctctccc aaagtgcacg gtccgctcat acacattggt ccggcccatc
     7261 gcgtcaatcc atttccacag catccccaca tcgccgccga cggcatggtg aatgccgcgg
     7321 gttacgtagc ccacggcatt ggtggcatag gcgtttgtgc cgaaatcctg gacggtttga
     7381 tggccgtccg catccaccac gatgcgctta tcggcattgt tgttatccag gagaagccgg
     7441 gaaagcatct ggctgttcac catactgcgt tcctcgtaaa tctcccccgt gaaagccaga
     7501 tttgtttgat acacatacca gatggcgcgg ccaggccctt cgtaatgagg atcgcgcgct
     7561 gtcttcataa gtgctttgcg atacagatcg tcggcaggtt gatagtagct gtattccgca
     7621 taagtcccgt ctccataatc agcgcggacc aacgggatgt ccaccgcctc aagactgggc
     7681 atccagttgc cgtattggta agttacgcag cgcccgtcgc tggccagtac cgatgagatc
     7741 agatacacgt ccacgacggc aacggaattc gtctgcagat ccgacgccga accggtgtag
     7801 gcccatgttg gattatccgg atcgatcctc cagtaatcgt tggtatcgcc ggcgtatcgg
     7861 acaatgaact tgtagtagtg ccagccgcga gcaagcgtct ccacgcgctg ccacacgccg
     7921 cttgtctggg tcatgggctg agctgaccag ccgttgaagc tccccgcgag caggacctcc
     7981 gatgcgttcg tttcgatgta gtagaactcg atgctccccg cgggcacgga gttggttgcc
     8041 ggttggtaac tgaagcgaag gtactggttg gtattgggcc cggtaacctg tgaaagcagg
     8101 ccattggaat aggcatacga atagcggttg gaaaatggat ctattgtggc ttccatgcga
     8161 aataacttgg tgccgccatc tgtctgttta gtgaaatggt gttttgtgcc gtctagaaac
     8221 atgaggtagt aattggtgcc atcgggaaga atgcgctcac ggcgtctttg gcgaaattcc
     8281 cgaacttccc atttggatat ataatccgaa tacgctcctg gccaatttcg ttggtcccga
     8341 aatcaagaat gctccactgc cagctgtgcc tccagtagcc cgcgccgccg aaaggggtat
     8401 caaaagcgat atgcgggtag gcgtcccatc ggctggcgaa gtggcgcgtc atttgcagtt
     8461 taaaggcccc gacggtatga gccagttcca agtccttgat ttcgcggccg acactgccgg
     8521 aaaagggatt gaatggattc gccccgcttc cacaactgct gcacgatgga ccattagcgg
     8581 caccacccca gcccgatccg ggcgcggccg gcgagtttcc gcctcctcca ttattctgat
     8641 tgcagcaatc gggatctgta gtagtccctc cgcaaactcc acaaacatca ggcggttgcg
     8701 gtggattccc gcatgacgag gcggcgtagg agcatcgggg gctgccggca actactgcgt
     8761 aaagaagaat cccggttaaa gcctttttca tcgcttctca tccttctttg ttacccagaa
     8821 gctgatctct tcttctcgcc cgccagaccg caggattaag ggataatttc ccgaccatgg
     8881 ccctatttgg aaccggaacg atatcgtccc ggttccgtcg gtcgtcatag aatccgtagg
     8941 tttgccgttc accaacccgc catgtaacgt gtacaggaag acgcttattc ccttcggata
     9001 gtgaggcttc ctggcaacaa caaccacttc ttcgtccggg tttagcgtta gttgatcagt
     9061 cactcccgcg tggttgccga gttggatttc atggttggtc cgcgtgacac tgatctcggt
     9121 ccaccctccc gccggttcaa tcgatttact gcttcgactg taggtgtcgg ccgcggagcc
     9181 cgacgccacg cgccgctggg gctcgtcgac agctcgatag ccggcggtac tcacaaaggc
     9241 acagcaggca tacatggcca atgaaaccca tatacgcttt tttttcatgg cagcacctct
     9301 ccgtctctgg ggtatgtaat tgcgactacc ggcgaagggt tgaatcctgc gggatcggtg
     9361 cccgcttggt attcttcctg attggaatag ccgtccgcgt cggggtcctc ctccgcgtcg
     9421 gacgggcctt cgggatcgaa tccattcgcg agttcccacc agtcaggcat gccgtcctcg
     9481 tccgtatcca ggaccaggtc gaacgagaac agatatttca tttgcccgat attggcggcg
     9541 gcatagtcgt tggtcgccga ggctccggcc cagggatagt cattggtata gccgatggcc
     9601 atcaggcgct cacagaacgg caaggccgta ttcttgagct gcccaatatt gaccggggcg
     9661 tagttggtcg tatttgaaaa cccggccacc aagttggaga tggccgtgcc cgctccgcca
     9721 aattgggcga tggaatcgtc cagttcggaa caggcctgaa cggctatgtg cttaagctgc
     9781 ccctggttga ctggggcata atctccagcc ggcgcgttgg tatccagcac cccgcgcgtc
     9841 gtccaccatg ccgggccctc tgcgcgggca tggagacaaa gcgcaaacag caagccaaag
     9901 gccgtcctgc cggccgtcgg catgaaagga acattcatct tggcctcctg tcttcttggc
     9961 gttatgggag gccattagtg aatgttccca tggacaggcc gccctgcggc aggacataca
    10021 gggctgaacc gttcccgaca aaaacgccgt tgctgatggc gcttccctgc atgttgaagt
    10081 gcccggtgac gcggatttcg ttgacgacat ttgatgtcac gtagaaactg gccgtgtagt
    10141 tcgtggtcca ggttgctccc gccgacgtta cagatcttag cttgagccac gacaggccat
    10201 agttgccggc ccagcccccg cccaaggaat agaacgagta cagatggtca atattgtcag
    10261 cacttgttaa tattctgcca ttattaatat aaatggaggt atagtagttc gaaagaacgg
    10321 ggccactgat atatggggga ttattggcaa atgtaatagt cgccacaccg cctatcttct
    10381 ttcctcctaa atttatgtcc ccagccagcg tgtcgcccgt gacgttcaca aatcggccat
    10441 ccaattcccc gacgttcaga tcgttggtgc cgacggtccc attctgaatc tgccagtagg
    10501 acacggcccc ctcgctgatg tgggccgact ggatggcgcc gctttgaatg tgcgcttgtt
    10561 gaatggcccc ggtggcgatc atggcggaag tgatcttgcc ttgcagcacg ccatcagcga
    10621 ccagagcata agcattggga tgaagcagtt ctcttgcttc gggcgccccg ttcacgacgg
    10681 gctcaatata cacttccccg cagcccaagg cttcagaaag cgttcccgtc gtgggcgtat
    10741 cccccacaag ggcagaatag tatccatccg ccacctggac ggtattggaa tcttcgaaga
    10801 ggcagacacc accaacatcg tttgtataga gccgcaacag gagttgaatc gtcggcccgt
    10861 tcgttgtctc ccccacatag acaaattgtt gaggcatttg cgcgagaaca gcgaaacaac
    10921 aggcaacgag caccatccat acgcacagca agcgacgggt catgacggtt tcctcccttt
    10981 attacgcgtc gagcaaactc gcggcggggt tcaactcgac ggctgtttcc tccgcacctc
    11041 gcaccttgta cgtttaataa tcgcttcttt ctgtggatac gtaagaaaaa aacgaatcaa
    11101 tcgcaggata ccttctgcca ggaagagagt catcagagtg aatctacctg cgcctgatcc
    11161 cctgggctct cattcgcgcc gatggtgtcg tggccctgga gaagcttggt ttcaggttgg
    11221 gctctcttcg gcggttgttt cttgtttgat atgggcttgc cttgccgtca gggctgcttt
    11281 tgggcgcgag ccaatgcctc gtcgaggcga cgcgcaagag gggtatacat ggagataaag
    11341 taaatgttcc gcctctccgg tcccggcaca aatccggcgg gaagtcctgc cgatgagatg
    11401 tcgagcgccg tactgcccgt gcggagatac tgctgtaaca acagggcata gtaaacagta
    11461 ttgagctcca agtcggacgc gcccgggttt tctttcaaga acgtcttggc gtaagtctgg
    11521 cgaaacgatt cggatggggc gcgggccggg aggtaaacac tcattgccga cccgtcggcg
    11581 aggggaacgg tcgtagcgca tgaaaatccg tctctcttca tggagggctt gatcccgggg
    11641 gcggagaaga ggatcagggc gtctgcgcct cgtgccgctt ggtcgcgatc gagcgtgcga
    11701 tagcagccgt tccagccgca gcggcgatta taggcgttag cgcccgcgag cgaaaaaagc
    11761 tccatgaact cctcatagcc ggtcgcatac tcgatgtgtg ggcgggtgaa attctccata
    11821 tccatcacaa ttccggggcg aaaggcccaa acgagcgcca gggattcatt cgggggaaag
    11881 ccggatatga gaaggctgcg cgtgaacaac catcggggga cgtcaataac gcccgcctcg
    11941 tccacgtacg gctcacggcc gggcatgttt tcgaagagga cctgctttaa ggcctcgata
    12001 ttcaaagacc ggttcaggta gtagggggca tatacagcac ggctggcggt gagcgcgtat
    12061 tgagtcgccg ccagagtcag cacgaccccg gaaacgataa cgcgggtcgc gcgagcgcgc
    12121 agccgatcca gacccttgaa aagcagcagc accgcgggta ccacgcaggg ggcgatatgc
    12181 cgcggctcct tcacgacctg caaggttagc agggcgatgg gtacgatcag ccacaaccat
    12241 aggtccccca cgatttctct atccgtcttg gataccgcgc ggtcgggcaa ggaggcccga
    12301 acggccagcc acaggaaaaa cggggccagt aaaaaaagac catagcgacc gaaggccgcg
    12361 ggatagtaaa gtgccgatgc cgagctgatc acgtcgggcg gaccaaggag ccgcatcgaa
    12421 cgcaacgccg tgtttcccgc gctcatataa taggcgaatc cggctggatt cagaagccag
    12481 aaaagcccga caaccactgc cgggggagca aggacggccc cggccagcca caggcattgc
    12541 cggccgtcgc gtgtccggat ggcgcgtacc agggtggcga ggactggccc gacaaaaaaa
    12601 acgggccatg tcattttaag cgcgaagccc aggccgaaca aaacggggaa gaaatcaaaa
    12661 ccggtacggc cggggggccg gcggcgtgca aaccacgccg cataggtaag cgtaaccagt
    12721 gccgtcagtc cgaattcgag gtacaactcg cgtgatagtc ccagcaggag gggggaggaa
    12781 accatcgtgg ccagaacgac aggcgcgggg atagtcaggg ggatgtgtcg gcacagcgaa
    12841 tacacacccc aagccatgaa aaagagccat atggcattag tggccagcgc gaatccaata
    12901 gatccgcaaa cggcaagggc aggggccagc aggaggggat agatggaggc aacgggaaat
    12961 ccccgcaggg tggcgccgcc cgaggcgatc cggccggccc agtgatagta ctgttcggga
    13021 tccatccagg aaaacgcgtc ccggggccac acatcggcac ccagatgcgc cgcgagcccc
    13081 aggagaagga gccccatcac taaacaagct ggatatcgtt tatgcatgct ttggccgggc
    13141 gattcgagcc cgctcggatc gtctgtcgta atactgcccg gcaagaaatt gcgcaatatc
    13201 aatcggcgca gcattccaac tgcgggtcgc gaaatacgac tgcggccacg ataaccttgc
    13261 cactctccac gcgcttacgc gcgcggctgc gtagccgtcc cggtaacggg gcggccggca
    13321 tttcatggcg gattagtaaa aaggctattt gaattgtcgc cattgcggag acattagtat
    13381 acgttcgcca ttgatgacac gcagaaatag cctttggcgc aagctggcca ttgcgttggc
    13441 tatgcctgta ttgttgcttg ttcttgccga ggtgcttgtg cgtctcttcg gggctaatcg
    13501 atcaggcagg aactcctggc ggtggggccg attctgggga tgccacagtt caggagcggt
    13561 tcgacaacta ttactccggc tacgaaatac ctgccgcttt ccaagcgctt acgcgcgcgg
    13621 ctacgtagcc gccccggtaa cggggtggca ggtatttcat ggtcggagta gtatgagcgg
    13681 tcgccgctca caagccgggc aggctgcggc atgcccatga gctgcttcga aaaagcggga
    13741 taagcgcagg ccggcatgga gattgttgca cagtctggga tgaaatcatc ctttggcgcc
    13801 gcccttggga tcggcttgtc gattattttg atctatggcg cgtgtctcgc tttctttatc
    13861 ccccgcgatg gtttttgggc tgccgacacg ggctgcaaat ttatacagat ggaggggctt
    13921 atccaaagtg gatatcgtgt ttttgatatt cgctggccgg gggcggccgt ggatcccgac
    13981 ttggcatggg cacccattcc atatcctttc ggtataaaga tcggagggaa gctctttctt
    14041 cagtacacgc cgatatttgc actgcttgct agtgtgccgt acagactcat cggattcgcc
    14101 gggctctacg tgctcccctg cttggcgggc ctcctattgt tgccggccgt atatggcata
    14161 gcgcgcgaaa ccggttcccg acccggcgcg gcgccgctag cggtcctgct gacggggctg
    14221 gccggccctt tgtggtttta tagcctttct ttctgggagc atacgctggc cgttgccctg
    14281 ttgggttggt ctatctgggc agggctgtgc caccttcgga caggcaccgc gctgccattg
    14341 gtcgtcgccg cgctgctgtc cggattggcg atctatgttc gagatgaatt atatcttttc
    14401 gccggcatat gggctgttgt gcagggcttc ctgtcgcccc gccgacgtat cgcatggcta
    14461 ttccccgccg ttgcggttgt taccctgata ccattatgga tattctatta ttgttcattg
    14521 ggtaatcctt gtggttatca ctttgtccag gcgaaagagg ccttcaatct tgcacgcttc
    14581 gtatccgaac gagtggtggt ttttcgcaat ctgttcctta acgcccatcc acaaccaatc
    14641 gtgtcactcc tgttgagcat acccttgctg tctctcgggc tgtttcgctc gttggatgat
    14701 cgctgggcgc ctgggatctt tctactcatg gggttgagcg ggctcttgat tttggttaca
    14761 cttgcggcct cgacatcgcc gatggatacg atgttgtaca ctaatgggct ttttgctccc
    14821 gcccccatac tggccgttgg gtttgcccgc ttcgcgccgg ccaagtgtgc gtcagtgatg
    14881 tctggctccc ggggattgaa agccttggtg tggctgactg ttggatacgc gatcggatac
    14941 ggctgccttg ccccggtggt cagttcgcag gggattcatt gggggtgccg ctttctgctc
    15001 cccgtctacc cgcttctcgc cattcttgcc gcgcgcgtca taacggcttt gtggagaaac
    15061 tcgctcgcgg gtaaggcgac cataaccgct gccgtgggcg tcgctgttct tttgcagttg
    15121 ttctcgatac gccttctcat tcaacgtcag acctatacga aaaacgtcgc gagtgttttg
    15181 tctgttcgtc ccgaaagagt ttttgttacc aatgtccctt tgctgcccac gacgtccctt
    15241 gcgcctctct ttttcagccg ccccatcttc ctcgtgcgca ctccggaaga gttgacgacc
    15301 ctgctggctc gtttacggga agcaggcgtt caggatgtgg cctggttaac gcacgagcca
    15361 ccccatagcg tacccgcacc gggcgccatt cgtattcacg atccctggcg ccgcttcctc
    15421 gctgtggacg ttcgcacgat ccgtgtggcg tcaccataaa atcagaagaa tggggaagcg
    15481 tccagcgcgg cggcccagtc ccgctcgatc gccgccatgg cggaccggcc gacggcgtcg
    15541 aagtaggccg caagctgccg gtgcgcgtcg gcatctctcg tgatgtcgaa taccagcagg
    15601 ctccgcgcga gcacggcaac cggcgtccgc gcgtgaagcc actcgaagtt gcgatgcccc
    15661 tcgtagtagg tgctctggta attcgtggcg gacaccacca gcagcgcccg ccgctccttg
    15721 cgcaggtccg gatcccggtc gtggaaatcc ggattcgtca cggagcccac cgagacgtgt
    15781 tccagaccat aggcgttcgg atcggccgtc ccgaagtacg acagataaaa tccgctgatg
    15841 ttctctttta gaacataatc acggagcgcc cggagaccct gcccccagtc cacgttggaa
    15901 tccgttgcca acttgtaccc gttcgcggga ccgccgaccg tctcgttgaa gtaggccaga
    15961 aaatacggcg cggaatacgc ggtcccggcg gcgtaccaga gccccagcgc ggccgacgcg
    16021 atcgcccacg gccggcgcgg caaccggtcg gccaggaaca gccccaccgc gagaaacagg
    16081 aacgggtaca ccggcaggag gtggcgatgc ccgatctgga tgtccgagcg gcagagcagg
    16141 acgaagtaga ccagcggcgg aagccacaaa taccacggaa tccgcatggg cttccgcagg
    16201 acggccgacg cggccgcaag caggcccagc agcaaaggca agggcgtctt gatgaggaag
    16261 agggccggga agtagtacca ccagccggtc tcgctgtgcc ggccgaagag aaatgttggt
    16321 tgtcccttct tggtcatgaa caggacgccc cgcagtccct cgacgaagac cggcaattcg
    16381 tgcccgcggt agatcagcgc cagcatcagg attaaagcgg cggcgaacca gccgacctgg
    16441 ctccacggga acggcaggcg ccccttgcgc gcgatgaacc agagggcgag aaccggcagc
    16501 gcagcgatcg cggtgaactt gctggcgagg gccagggcca gcagcgtgcc ggcccacacg
    16561 gcatcccgcc gctgcccgcg ctgctcccag cgcccccagc agaggaagaa caggaagaag
    16621 aacgcggaga ccgccaggtc cgtggtcacg aggctggccg ccgccagcac cgcggggttc
    16681 aggatgtaga ggaagaacgc gatccaggcc gcgcgcggat tgaagaaccg ggcgcagacg
    16741 ccccagatcg cgacggcggc gaggccggaa aagaccagca cccccgcgcg cccggtgttg
    16801 agcaggcggt cgggatccgt gcccgcgccg ttgtggaaga gaaaccgatg ggccagcgcg
    16861 taggcgggat tcggatcgcg gtagggcgcc gcgacgtatt cgcggggtac ttccggcttc
    16921 agcccgagga gtggaagcgc ggccatgagt tgctggacgg gcggatggta cagcccgttg
    16981 aagcaatcct tccccgtcgc caggaaaatg tatccgctca agaggtgcgg gggctcgtcg
    17041 aagacggccg actgcgcccg gatcgcggcc ccgcccagcg ccaggtgcag caggaccccg
    17101 gcggccaaaa acaggaccgt gacggccctg cttttaccgg cggaactctg gatcagcgcg
    17161 tccggcctca cgccccgtgt tcctagcagg ggtgggcgcc ccgttcaacg agaaagggat
    17221 ggcgccgcgc ctacgaacac cccatcgagt tgccgcagtt gtagcagcgg tagcaggcgc
    17281 cgttgcggac ggtgatcgcg ccgcactggt cgcagaccgg ggcgtcctcc atgaagttct
    17341 tgaactgcgc gtcgagaacc tggacgttca tcccgtgcgc cggggccgcg gcgggtttgg
    17401 cgggcttgtc tgtggccggg accgcgaccg ccggttcggg ctgcagcgac ttcgccgggg
    17461 ccggggcctt gaagccctcg aggaacgtgt ggccgagcca ccggaagatg tagtccggca
    17521 gcgacttggc gatcgggatg tccgggttct tggtgaagcc gctgggctcg aaccgcgagt
    17581 gcgcgaattt ctcgaccagc gtcttcagcg gcacgccgta ctggaggcac atggaaatcg
    17641 ccgtgccgaa ggagtccatc agcccgccga tcgtgctgcc ttccttggcc atggtgatga
    17701 acaactcgcc gggcgagccg tcgtcgtaca gcccgacggt caggtagccc tcgtgcccgg
    17761 cgatgtcgaa cttgtgggtg atcgactgcc gcgtgctggg catccgcttg cgcagcgggc
    17821 gcgccttgga gccgttgccg cccggtttct cggcggcctt ctcggtcttg cccgtgttca
    17881 ccggctggct gcgcttgcag ccgtcgcggt agatcgcgat ggccttcaag cccagcttcc
    17941 agccgtcgat gtacgtctgc atgatctcgt ccacggtcgc ttccgtgggc atgttgacgg
    18001 tcttgctgat ggccccggag aggaacggct gcaccgcgct catcatccgc aggtgcgcgc
    18061 cgtaggggat gtaccgccgc ccgttgcgcg gcttgaaggc gcagtcgaac accggcaggt
    18121 cctcgtcctt gatgcccggc gcgccctcga tggtgtcgtt gtgctcgatg tactcgagga
    18181 tctcctggac ctggggccgg ccgtagccga gccgctggag cgcggcgggg acggtgcggt
    18241 tgacgatctt cagcatcccg ccgccggcga ggagcttgta cttcacgatc gcgatgtccg
    18301 gctccacgcc cgtcgtgtcg cagtccatca tgaacccgat cgtgccggtc ggcgccagga
    18361 cggtgacctg ggcgttgcgg tagccgtgct tcttcccggc ctccagcgcc tcgtccgccg
    18421 ctgcgcgcgc ggcggccagc aggtcgggcg ggcagcccgg cggcagcgtg tccagcgcgg
    18481 cgcgatgccg gccgacgact tcgagcatgg tctcccggtt ttcggcgaag ccctcgaacg
    18541 ggtcgagcgc ctccgcgagg cgcgcggact gcgtgtacgc cccgaagtgc atgaccgccg
    18601 tcagcgcgcc cgcgatcgcg cggccgcggt cgctgtcgta gggcagcccg agggccatca
    18661 gcagcgagcc gaggttggcg tagccgagcc ccagcgggcg gaaccggtgg ctgttgagcg
    18721 tgatggactc cgtcgggtag ctcgcgttgt ccacgatgat ctcctgcgcc gtgacgaaga
    18781 tccggaccgc ggcgcggaac cgttcgacgt cgaagccgcc gtcgtcccgg acgaacttca
    18841 tcaggttcag ggaggccagg ttgcaggagc tgtcgtcgag gaacatgtac tcgctgcacg
    18901 ggttgctgga gttgatcggg cccgtgcggg cgcaggtgtg ccaccgctgg atgctgtcct
    18961 cgtactgcac gcccgggtcg ccgcaggtcc acgtgccctc ggcgatccag cgcatcaggt
    19021 cgcgcgcctt gtacgtcggc cccgacttgg cgggatcggt cacccagcgc gtcggccact
    19081 cgccatcctc gagcaccgcg cgcatgaacg cgtccgacag ccgcacgctc aagttctcgt
    19141 tctggtagcc gatggacgcg taggcctcgc cgttgaagtt gccgtcgtag ccctgctcga
    19201 tcagcgccca ggccttcttc tcctcgttct gcttggcctg gatgaactcc ttgacgtccg
    19261 ggtgccagca tttcagcgtg ttcatcttcg ccgcgcgccg cgtcttgccg ccggacttca
    19321 ccacgctggc gatcgagtcg tagaccttga ggaacgagag cgggccgctg ggccggccgc
    19381 cgccgctcaa cttctcgcgc tcgctgcgga gggtggacag gtccgacccg gtgccgctgc
    19441 cgaacttgaa gagcatggcc tcgctgtgcg cgagccgcat gatgccctcc atcgagtcct
    19501 cgaccgactg gatgaagcag gccgagcact gcggcttctc gtactgggtc tccgcgcggc
    19561 gcacgcggcc ctcggcgcgg tcgaagtaga gcaggccgtg cccgttgccc ttgccggtgc
    19621 cgtactggtg gtgcagcccg cagttgaacc agaccggcga gttgaacgag ccgtgctggt
    19681 gcaggcagag ccacgcgagt tcgttataga aaatctccgc gtcctccggc gatgcgaaca
    19741 cgccgtccgc gaggccccag tccgcgatcg tccgcgcgac ccggtggatg agctggcgcg
    19801 cggagtgctc gcgcgcggcc gtcccgagtt cgccgtagaa atatttggac gcgacaacgt
    19861 tcgtcgcgag catggaccat gcgcgcggca ccatcacgtt gtcctgctcg aagatcgtct
    19921 tgcccttctc gtcgccgatg accgccttgc gccgctccca ctccaccgcg tcgaaggggt
    19981 tcacgcccgt cgtgctgaac acgcggggaa tcgcgaggcc gggccgggct ttcttcttgg
    20041 ccgccggggc ggcgcgccgg gcgcttttcg ccgcgggccc tcgccgtgaa tgcgccgcgt
    20101 ccttgtcgca aagctccacc gattcgcgcg tgtccaacat gaaaatcctc cttattacaa
    20161 ctcgctgaaa acaagacggt tatggctgcg aacgaaccag gcgaaccacc acatattgat
    20221 gcttcgcacc atgacctcgc aaaatacccc tatatatagg ggcgtcaacc ttaaaaagga
    20281 acttaaagaa aaatgggcgg aatggctttg ctttcagccg gattgtggag gcgccacgcc
    20341 ctcgcggcgt cagcgttacg gatggtcgcc ggtatggctg gccttcaact gcagaacgat
    20401 cggcgcgcct tcgagcccga acgcgttccg cagttcgcgg accaggtacg cgcggtaggc
    20461 gggcgtcatc cgtttgggat cgttgacgaa gagccggatg gtcaccggcg ccgtttccgt
    20521 ttgcgtggcg tagtaatatt tcatccggcg cccgcggatg tgcggggcct ggacccgcgc
    20581 gacggcgtcg tgcagcacgc ggttcaacag gccggtggtc agcttgcgcc gggtctgttc
    20641 ggccacgcgg tcgatggcct cgatgctgcg gcgcagcgag tagcccgtca ggctggacac
    20701 gaacaggatc ggcgcgaagt cgaggaaggg caggacacgg cgcagcgcgg cctcgtactc
    20761 gtcctccttg acgcccctgg cgaggtccca cttgttgacc agcagcaggc agcccttccg
    20821 gtgctccagg atctgcgcgg ccagcttctt gtcctgctcg cccggcccct cgacggcgtc
    20881 gagcatcagc acggccacgt ccgcgcgctc gatgctcttc tgcgcccgga acaggctgaa
    20941 ccgctcgacg gcggtgtgcg cgcggccgag cttgcgcgcg ccggcggtgt cgatcagcag
    21001 gtatcgccgc gcggattcgc cggcgccgac ggagaagggg acgtcgatgc tgtcgcgcgt
    21061 ggtgccgggc acgtcggaaa cgatcacccg gtcgctgcct agcaacttgt tgatataaga
    21121 ggacttgccg acattcggcc gcccgacgat cgccaccttg aggggcggct cctcggcgat
    21181 gtcggcgggc ggcggcaggg cggcgaggat gggcgccatg agcgcgtcga ggccgcggtt
    21241 gtgcagcgcg gaaaccggga acacgggaaa gccgaggctc tcgaactccg aaacctggtc
    21301 gtccagcccc gggtgatcgg ccttgttggc cgcgatgacg gtgcggcgcc cgctggcgtg
    21361 aagccgccgg gccacctcgc ggtccagggg cagcacgccc gcggtgatgt ccacgacgag
    21421 gatcagcgcg gcggcttccc ggatggccag ttccgcctgg cgctgcgtct cggcctcaaa
    21481 gaggtccttg gccgcggacg cgtccatcgt ggaaagcccg cccgtgtcgg tcagctcgaa
    21541 gcgacggccg gcccattcgg cgcggcattc cagccggtcg cgcgtgacgc cgggctcctc
    21601 gtggacgatg gcgatgcggc ggccggcgag gcggttgaag agcgcggact tgccgacgtt
    21661 cggccggccc acgatggcga ccacccgctg gggaacgggc tcgctcatgg cggggagggc
    21721 atgcgcccgg gagcggcgga gtgtttctcg agttggcgca ggccgtcgaa caggtaccac
    21781 acgcccgaga tgaacgtgaa cgaaccggcg agcaggatcc atacggcgaa cagcctcggc
    21841 cggccctgca tgagcaccca cgttatggtg atcatctgga aaaacgtggc gaccttgccg
    21901 accacgcgcg gtttcaccac cacggatccg gcgacggcat gaatcacggc cgcgccgagg
    21961 atcagcacca cgtcccggct gatcacgagc agggtgaacc agacggggat atgcggttcg
    22021 agcgagggca gggccgggcg ggtgagcagg atgagggcgg acagcagcag ggccttgtcc
    22081 gccagcgggt ccaggatctt cccgaggtgc gtgatctcgt cccgcgagcg cgcgagatag
    22141 ccgtcgaggg cgtcggtggc ggcggccacg atgaatacga ccagggcggc gatccggtag
    22201 ccgtcctcgg cccggccaag ggcgagtccc tgggtataat agaggagcat caggatgaaa
    22261 accggcacgg ccaggatccg gaggatcgtg acccggttgg ccagcgtcat gtggtgactg
    22321 gtctggtggt ccgccgtcat cggtaagcct cgactcccgg gccatgtacc atgccgggtt
    22381 cccgaaaagc acgctttttc gcggcgccgg ccgggttccg cttggctccg gcggcggacc
    22441 ggggtaggat tagtcaaaga aaaaggatga cgatgcactg gctgttgctg atggtggtgg
    22501 gcttggctgt cggggccttg agcgggctcc tgggcatcgg cggcggcgtg atcgtcgtgc
    22561 cggtgctggt gatgggcttc ggcttttccc agcatacggc gcaggggacg agcatggcgc
    22621 tgttcgcgct gccgatcggc ctgctggcgg ccatcaccta ctatcgccat ggccacgtga
    22681 acctgccggc cgcggggctg atctgcctgg gttttgtgat cggcggcctg ctgggcgcgc
    22741 gcctggccgt gatcctcccc gaactgctgc tgcgccgcgt gttcgggata tttctgctgg
    22801 tgatatcggt gcgcctgatt ttttttaaat aaggatttca tcccatggct tcggtcagcg
    22861 tccgcgaagg ggtctgccac gccctctatg ttttcgacat cgggctctcc attcgcctcg
    22921 atcgcgtccg gggtctgctg gcgcggtcgg atgtgaagac cgaggcgacc accagccgcc
    22981 gggccccgaa gtactacggg ggctaccagc ccgcgccctt gcacgtcgcc cagcgggccg
    23041 aggccctgca ggtggccgac cgcgccctgt cctccgccgt ggacatggtc ctgtacgact
    23101 tcggcgcggt gaccgtggcc tacgccatcc cgttcgaggg ccccctttcc gaactgggcc
    23161 gccttagctg cgagctcaac gccggggacg cgctggagcg cgacgcccgc ggccgcgtcg
    23221 acggcctgct gcgccgcatc cgtcgcgccg tctcgcgccc gcgcccgacc ggcccggtcg
    23281 aggactacct catgttcgag gtgcgccagg
//
LOCUS       DAOOTK010000039        23048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1541_length_23048_cov_4.891228, whole genome shotgun sequence.
ACCESSION   DAOOTK010000039 DAOOTK010000000
VERSION     DAOOTK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23048
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_23048_cov_4.891228"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1729
                     /locus_tag="PK634_06395"
     CDS             5..1729
                     /locus_tag="PK634_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOW97402.1"
                     /translation="MVTKAPAGSTIMDAADSVGIKIPRLCYHPHLSILGACRVCLVEV
                     EGQRNPVASCAYPVADGMKVRTSSNQLRRLRRDVVELILDSHPKDCQTCERNGNCELQ
                     KLSYDLGIRERLFEGERKQPPKDLSSPAVIRDPEKCILCGRCVRVCNEVQGVYNLSQH
                     QRGFQICVAPAHEGDMLNSVCVQCGQCANVCPTAAIIEHSATDEVFKALEDPAKHVVV
                     QTAPSIRAAIGEGFGFEPGTPATGKMISALRTLGFDRVFDTNFGADLTIVEEAHEFIR
                     RLKNNDRLPLITSCSPGWIKFMEHFYPELIPHASTCRSPMTMLSVLLKTYYAEKQGLD
                     PKNIYVVGVMPCTAKKYEARRPEHADANGVPYTDAVLTTRELIWMMKTFGIEFKHLHD
                     GDFDNPLGFSSGAADIFGTTGGVMEAALRTAYETVTGQPCENLDFVEVRGVEGVKESK
                     VKIGDQTLNVAVSNGLQNAKRLFDRVLKGDKKYHMIEVMACPGGCIGGGGQPYPPPGL
                     HIMDAKLLRMRAKALYAIDHHKQIRKSHENPYIQQLYKHYLGEPNSPKAHELLHTHYE
                     AKLPRGTR"
     gene            1726..2244
                     /locus_tag="PK634_06400"
     CDS             1726..2244
                     /locus_tag="PK634_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOW97403.1"
                     /translation="MTAQEEKVTTLAELPEPIARFIDETRDRPNADSYLVAVLQMIQK
                     HFGYLPAGALNTVSQKMQIPAAKVTGVATFYHFFTFVPKGKHTVSVCMGTACYVKGAG
                     ILVDRLKDLLGIRQIGHASKDGQFTLEVARCVGACALAPVLIVDDKVYANVRPDELPK
                     ILSKYGFKPKAE"
     gene            2292..2717
                     /locus_tag="PK634_06405"
     CDS             2292..2717
                     /locus_tag="PK634_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW97404.1"
                     /translation="MSDGKTILIVDDDPDFVESNRDLLEAYGYSVHAAHNGADGLDLA
                     RKVRPDVLILDVMMATNTEGFEVARKIPETPELKGLRVLLVTGVTKDMHLPFGFEPDA
                     SWLPVDRVLEKPIAPNRLLTEIQKVLGEANPKGENSHAG"
     gene            2707..3099
                     /locus_tag="PK634_06410"
     CDS             2707..3099
                     /locus_tag="PK634_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW97405.1"
                     /translation="MPASKKTILIVDDDRDFVESNKDLLEANGYRVISAHDGASGLAL
                     AKKERPALMILDVMMATNTEGFEVSRRIPESPELKNMPVLMVTGIRKEMHLPFAFEPD
                     ETWLPVSSLFEKPIEPATLLAEIKKRIG"
     gene            complement(3127..3696)
                     /locus_tag="PK634_06415"
     CDS             complement(3127..3696)
                     /locus_tag="PK634_06415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97406.1"
                     /translation="MTAPHKHHACPVWIGWLLLNPLRRLLQDPRKILAPCVRPGMRAL
                     DFGCAMGFFTLPLAELVGPTGRVYAVDVQDGMLDQLKRRAARRGLSGRIFIRRCGHED
                     FGLQEFEGAMDFILAFAVIHEVPDVQRLLAAFHRALKPGGTLLIAEPKGHVDGPAFEA
                     EVRIAKETGFILRTTPRVGCAYAALLAKQ"
     gene            complement(3703..4692)
                     /gene="hydE"
                     /locus_tag="PK634_06420"
     CDS             complement(3703..4692)
                     /gene="hydE"
                     /locus_tag="PK634_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOW97407.1"
                     /translation="MLSKNDILDELRAPDSHALRGRADAARREHCGEGVFLRGIVEFS
                     NCCARSCRYCGLRRENRRLKRYRMSPAEIRQAASTLLRWGLRTVILQSGDDAHYTTAM
                     LCDIIRDIKTDAPGAAITLSIGERPLEDYEAFREAGADRYLLKHETVNATLYDRIHPG
                     QSHAVRMSIIERLRELGYQVGVGFLVGLPGQTAEDLAGEILFLQDFQPDMAGIGPFLP
                     QADTPFADQQAGERETVLRLLALARIASPGTHLPATTALATLDPSEGFAAGLRFGCNV
                     IMVNGTPDSYRASYRLYDKRVCADLESARRAIRLAGRTASLERGDSLRLQPAE"
     gene            complement(4777..6273)
                     /gene="hydG"
                     /locus_tag="PK634_06425"
     CDS             complement(4777..6273)
                     /gene="hydG"
                     /locus_tag="PK634_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOW97408.1"
                     /translation="MTTQLTERSSWIRNRIARDQIDKYRTDDGDFIPDAEIEARLRAN
                     REPDPARIRAIIAKSEAIQTLEPDETAALLHVTDPDLWTEMKTAAARIKRKVYDNRIV
                     TFAPLYLSNRCVNNCLYCGLRCANAAIERRVLTQDEIRREAEVLAGQIGHKRLIVVYG
                     EHPDSAIDYMAESLRTIYSARVKARIGFGQIRRINVNAPPLRVEELKILHEVGLGTYQ
                     VFQETYHHDTYARLHPAGTIKGDYAWRLYAMHRAMEAGIDDVGLGALFGLADWRFEVM
                     GLLYHARELERAFGIGPHTISFPRLEPAESTPFTQESGFLVSDEDMERAVVVLRLSVP
                     YTGMILTARENAGMRRRLIPLGCTQTDASSRIGVGAYSEAYESQQGSRQQFLLGDTRS
                     LDDVVRELAEMGYITSFCTAGYRCGRTGQCIMDLLRSGREGKFCKLNAVLTFREWLDD
                     FASPATQAAGEKVLQHELAEIREQNPKGYPVFMQFYERTARGERDLFL"
     gene            complement(6288..7550)
                     /gene="hydF"
                     /locus_tag="PK634_06430"
     CDS             complement(6288..7550)
                     /gene="hydF"
                     /locus_tag="PK634_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOW97409.1"
                     /translation="MEKTPKSLRLHIGLFGRTNVGKSSFLNLVAGQDVALTSPIPGTT
                     TDVVEKAMELLPVGPVAFLDTGGLDDTSELGAGRVDRTRRALDRSDVAVLLLEPNRWT
                     AFEDELLGEFTKRKTPVIAVINKADLEQPTAAFIEAVRAKIPRLLQCASHPFTGAERD
                     RVVHQFKEHLLEICPDHVVAAPSLLGDLVPPGGLIVLIIPIDKEAPKGRIILPQVQAI
                     RDALDHGEMVAVCRETEYTALLSQLARPPDLVVCDSQVVHRMVAETPPGVKCTTFSIL
                     FSRFRGDLARQTEGVSAIDRLRPGDRVLIAEACTHHAVEDDIGRVKIPRWLKKHLGFD
                     VEVTVCSGRDWPDDVASYSLIILCGACMLTQREVLARIEKAAAAGVPITNYGLCISHL
                     QGVLPRVLEPFGIEQKAQSKTAWAGAPS"
     gene            complement(7614..7862)
                     /locus_tag="PK634_06435"
     CDS             complement(7614..7862)
                     /locus_tag="PK634_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOW97410.1"
                     /translation="MKKRLGFVGIVVENRKKSAGLVNQVLTEFGNLIYGRMGIPHLEE
                     DHSVITLIVRATTDEVGSLTGKLGQIEGVSVKSAMTSK"
     gene            8049..9146
                     /locus_tag="PK634_06440"
     CDS             8049..9146
                     /locus_tag="PK634_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW97411.1"
                     /translation="MSRMRIAFLLQDTGRIYGAERATLDLARGLRSAGVEVAAWLIEE
                     SRLGLEKSELKTAILEAGLECSALPVHSAFSRDLLRMLREKAGREKPDVLHTIGPKAT
                     VHAAWALRGRVPLVTTVHGWLFRPDLKERFYEWLELRAFRRFDRVITLSRHYEQWLAA
                     RVGAGRVVRIPSGFDARSVAAVGDRGHQPMTFGMLGRLSWEKNHELFFRAARRLQDDK
                     ASARFLVAGEGMERPRLEALRAGLGLESAVEMAGYVASEEFFRQVHVLVQCSRMENLP
                     YSIMEAMARERPVLATRVGGLPDLVEDGVTGFLVEPGDDAALADRMRKLSDDPDLALR
                     MGKAGRAKLQQEFAPEACIKQHLELYGELTA"
     gene            9150..9860
                     /locus_tag="PK634_06445"
     CDS             9150..9860
                     /locus_tag="PK634_06445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW97412.1"
                     /translation="MSEPLVSIITSTYNRSPMLRRAVESVRAQTFTDWEHIIVGDCTP
                     DDTEAVVASFNDPRLRFFNLPEKSPPGSHGAIAKNHGIQRMARGGYIAYLDDDDRYRP
                     LFLEVMMGYLRVHPDAALAYCRSMYRDKDTGHRLWGNPFQRWLHGYSKEKLRRYNFLN
                     TNCVIHKKSVAEEVGGWDPGYYFDDYELWLRISERHDFHYVNRVLVETFVSEPSFLVR
                     AVTKGWRILRHGRTTPVN"
     gene            9933..10697
                     /locus_tag="PK634_06450"
     CDS             9933..10697
                     /locus_tag="PK634_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97413.1"
                     /translation="MIGGKKITVVMPAYNAAQTLEKTVQALDRQVVDDIIVVDDCSSD
                     ETVARAKELGLFVHRHERNLGYGGNQKSCYRLALQRGADIIVMVHPDYQYDPRLVPAM
                     ASMIASGLYDAVLGSRILCGGTLAGGMPYYKYMANRLLTAFQNILIGQKLSEYHTGFR
                     AFSRKVLETLPLEENSNDFVFDNQMLCQAVAFRFRLGEISCPTKYFAEASSISFRRSV
                     TYGLGVLRATMALALHQRGWAKNERLFGEQGRRLTP"
     gene            10694..11680
                     /locus_tag="PK634_06455"
     CDS             10694..11680
                     /locus_tag="PK634_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97414.1"
                     /translation="MSEEASGAPGCPACGAAWGNEVMACTASDGREFRYGRCPGCGLY
                     RLTTLPGPAEIAGFYDAAYYGAGERKFPALMDQVRNLFLRRRATRVAGWAGRAAGRAL
                     DVGCGDGRFLGHMQGLGWQVAGTELPGAAFDRAQKIPGIQLKAGTLTGGLFPPASFDA
                     ITIWHVLEHVPDPLAMLRACRELLAPGGLLVVEVPNTDSWQNQLAGPLAFNVDPPRHL
                     YQFGPESLGRLLARAGFSIRRRETSSLEMGVIGAAQSVMNGLIKPRDLFYDLLRSRGK
                     CPGAPAAKWISLLLAVPLVPAGLLFTVAETLAGHGPALRLICEGAANSSGGS"
     gene            11700..13322
                     /locus_tag="PK634_06460"
     CDS             11700..13322
                     /locus_tag="PK634_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW97415.1"
                     /translation="MKDRFLVVLGENLRLCGAGGRFLDYGCGRGDVAEYLIRGGGFTE
                     GLAYDPSVTAGQESSSGPREGGRQLAYRRTLPEARGDFDVAVLFDVIEHVPEPARVLE
                     DIRAQVKPGGWLAVTVPYNPHEWGVDDDFYGHLRRLSMRGVVSMLEAGGWGVIRALDP
                     SFPSFWFIRRVYLMTRRFLRQPIPAGADVAGTDMHRSLASSRQSAWDTGGPVQRMLSG
                     GLLPWKLIRRFDLYFESIFRGFELFILCRKREGEASCGVCGCGRFVYHRFFHRYSLQR
                     CTYCETEKILAADSREAGSDGLRPSPVHGLIRRWRARRVRRLAGRASGAMVAVVRNPA
                     EDIDLAELQAGGREVRCLGLPEWLAAPAQSCAVVALFHVIELAPEVDMVLDRLDAAVA
                     HGGHVLLEYPNSGSWLKRAFRWRWFGYDPPNHRHIVDSRKLCDRMGLRNYHLVRERHF
                     APEYSFFIFIQTLINVLLSFQRDALYRWLRGVGTSPVDRAAAWLSIPLAVLLAPLFVI
                     YQPLASWFRRGCVVQQVYRRTDIASASQGGGA"
     gene            13326..14723
                     /locus_tag="PK634_06465"
     CDS             13326..14723
                     /locus_tag="PK634_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97416.1"
                     /translation="MDPEIPVRSGGGVAWLDRHYLFLVTVLLLLVYMFTYFGLNQLPI
                     RDDGAGYYAYLPSLLLYGDPSFETDARAHYGGEFPRWTYVRRYPPTGRYLNSLNIGVS
                     VMTAPFFVAGHALTYWFGWPWKGGWPWMEMRYPPDGYSMFYQHAAGLAGLFYSLIGLV
                     LWKRLLARRFSKGVVLATMTALLLGTNLLNYVAAETVHAHSFTFFLAAALMTLTEAWY
                     ERPGRLQVSLALGAVAALLFLVRQLNVLVLLWVPLYGIVGRRDVGARAVFFLKNGRDI
                     AVMGLTAFAVMIPQFLMWKYSSGHFIVKAYQHMGASSFGSPQVLDVLFGVYKGLFVWF
                     PIFVPAVLGILCLKGPAAAYRLPVIVIFAVYILAISSLKIWTSAGGFGNRYVVDTVIL
                     MGFPLAAFFSSLSSRWTRRTVAVIVLLCIFQALFLLTLLYRREMTFYGLDAQGFFDVF
                     WWRKESLVNWLRTRF"
     gene            14923..>23048
                     /locus_tag="PK634_06470"
     CDS             14923..>23048
                     /locus_tag="PK634_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97417.1"
                     /translation="MNYHHFTRRRWNSMGWPTLLLIPLFMAAGTAPADAATYYVNDAS
                     TNGDVYCSNMGSDANPGTNAAAPKLTLTNLFASVTLLPGDMVYIDTGGYSNYTVNVPA
                     SGTAGNLITFQGSTNYGAGGTVFRQNNAASNVFSLAGRSYIGLRDLTVRDGQCGVYMT
                     GSFCDFHRITAIRNSRGFWSQSGGVNAMRYCVAAENVIGVSGFDTTTFNQGVLWSNTT
                     AISTYTSSSIALSNSVIVGGIAFTLLPEAAEYNVYWRTDMSSNTVPSVRTLSEMQEAM
                     GHSFRSIVADPQFGDPAGYDFHPRSVTGRYVQATKTWTTDAVHSVLIDFGNPASAYVN
                     EPAPNGSNINAGVWGNTAEASKSRTNAWLLAVSFNDGGTLQQTGRLYWTAGNIPTTEK
                     VRLDYSRDSKATWRMIATNVAVTNLYYAWDASAVTSGAVMYWRVARETNTNFFDVSDT
                     NFALRGLTPVPYYVNDTGTWGDVYTHFPGSSANDGLTPDTPKDTIQGILDAYSLGGDH
                     TIYVDTGYYTGQTVVVTTSDSGWSNRYMTIQGSTNYLYDGTVLNRGSAAADVVILSAV
                     RRVKLRDLTLLNGRYGVFASSSLDCEYERLTARFNDTGFRGSSGNNHVFRRCAAAQNT
                     TGAAGFNLSVLWDYGVSWSNTTAFAPSVPSAWSVSNSVIVGGTAFGGAAAPGGGDYNL
                     FWNAAIHSSYTSLSALQKGANGWWNCAYREPFFVDPTNADFHPQSMVNTYSNGTWAKY
                     ANHSACIDLGDPAGSYALEPWPHGTNVNLGIYGNTAEASQSRTNAWLMAMNYNDGGVL
                     NAQLGERVYWKFGDISTTNRVRIELSRDGGATWVVAGSNILASIGYWDWANTNFASAR
                     LARWRVVLESNTNIFSATSYTNFTFQNGRYEYYLNDENITNDVYCTGPGSDTNTGTSA
                     GSPKYSLKSLVDAHEIQPGDIIYYDTGLYHYSADQAVSSLDTGVSNLPVTIQGSTNWA
                     AGGTVIERRTGPYGLDLSGAAYYWIENLTVTNAQTAVRIQGTNGLRLVRVSVKRSGNG
                     FEVASAADVVLEHCIAADNTGNGLLVSGAGSDVQYIRGVHWQNQGAAVRVAAGRVAVS
                     NTAIVVSHPTAFGYHAATPTNIVGDYNALHVESNGVAAYLTSLGRNLDSLDAWSVETG
                     QERMSLRTDPLFANPFYGDFHLKTDQRDSRWITGSGWYPGWDAQTSPLIDAGDPASGF
                     TNELAYNGGRINIGRYGNTAEASKRGMSNRLYAASLRQGGWVRGTSTLHWVVGGAATG
                     HFVKIEFSPDGGASWLTLTNRIAASAESFAWNTWAVTNTPAGLWRVTSTNTPALFDQP
                     TNFFAVRNASLSVYINDASTNGDVYAAAVGGPTNWQATAARPLNSLPRALTAFDLEPG
                     DTVYVDTGAYTNSEDTVFLRRHNGSSAQPLLVLGSTNAAAGGSVLTRAGDGATVNNLR
                     FDYAQFVIVSNLALRGGRTGVRMNGGGQVQLSRVDVSGCVSNGVEVLNSTNVLLSRVA
                     AYNNPGYGLNVFMANGVQWLQSVLWSNAAGAAKIVNGSLVVTNSVLEAAVDGTYVFTL
                     ASNAVVRSDFNDILARNGAYPALDGFYVYKNLSRWKDRTTNDIHSLTHDPLFVDAANG
                     DFHAFSQAGRFNPTSLTFVADSTNSPLIDTGPTDWPFAAETGTNGYRVNLGLYGHHGQ
                     ASRSRTNGWLLALTLNDGGSIRGTGTIYWVAGGVTTGQQITIDYSWDAGENWTNIATN
                     VAASAGNIPWATDRVRATSQGVLRYRSQPDASVEAMTERLFSINNTSLTFYVNDAYTN
                     GDVYTTAPGQTNYDGLTEDYPADSILTILRRYDPQPGDRFLVDSGTYVMPSSVTIDSS
                     TMGSLTNLVLFQGSTNQAVGGTVLDFGGQVYGFSIENTEGINLRHLRIQNARSGVRLR
                     VVTNCFVESVWVRGGGHGFEIEGSRNVELTRCAAVGAATSGVYNVFSSNTIWRNGVLW
                     SNAIGVLLANVSAVPNPPANYMVFSNSVVGAFGTSAVAYTINSGVLLADYNGLHLAEG
                     ARMARSYRVPFEVLSDGVARWRAWSTQDLNSVAGDPALCDPGAMDFHPLSPRGRYSVL
                     GKGFIATDTVASILIDVGPTNATDWASESSPNGSRVNIGMYGGTVEASRTPTNSFLGV
                     ISLNDGGVASGTNFPLRWMARGNATGHLVRLDFSWNYGTNWQTIVTNWPARAGTYTWN
                     TTNFTSTLFGLWMVSDETELGLASVSAQPFALRNTNFLFYVNDTGTVGDVYCDAAGAE
                     GQTGRGTNVPMLSIQRVLDAYDLEGGDIVYVDTGVYTGQDARLTVSQLDTAPPGGDTR
                     LCLVGSTNALAGGSVLDGAAGLFGLNMQEAYGVDARDFSIQNMASAAVRVAQSGQCAL
                     YNVQTRDGAVGFDLQRSIDSLLSQCSSVGSDTGLRNLYATNTVLQNCVLWSNRLAVSL
                     GSYAGAAMRNGVVVSNSVLGLFGAQRFAYEGETASLQADYNNVYLQGGAYAARRPGLT
                     FSETIDTVSRWVAQAGQDRHSLSHDPGFAQDGVDFHPKSARGRWAVTNYVYTDVTNSI
                     LLDAGAPGTNYFNEPSPNGSRLNVGPYGNTTQASLSPTNARLTVVSLNDGGRALGTNQ
                     YLYWVASGDATGHTVKISYSGDGGATWTTLVSGVSALASNYLWDTTTWPSTLLGMWEV
                     RSQTDTNIFGRNSTVFSVRNEPMRFYVNDANTNFDVYCTAAGHSTNNGLFAHTP"
BASE COUNT         3886 a         7483 c         7830 g         3849 t
ORIGIN      
        1 cggcatcgtc accaaggcgc cggccgggtc cacgatcatg gatgccgccg actccgtcgg
       61 gatcaagatc ccgcggctct gctaccaccc gcacttgagc atcctcggcg cgtgccgcgt
      121 gtgcctggtc gaggtcgagg gccagcgcaa cccggtcgcg tcctgcgcct atcccgtcgc
      181 ggacggcatg aaggtccgga cctcctccaa ccagctccgg cgcctgcgcc gcgacgtggt
      241 ggagctgatc ctcgacagcc acccgaagga ctgccagacc tgcgagcgca acgggaactg
      301 cgagctccag aagctgtcct acgacctggg catccgcgag cggttgttcg agggcgagcg
      361 caagcagccg ccgaaggacc tgtcttcgcc ggccgtcatc cgcgacccgg agaagtgcat
      421 cctgtgcggg cggtgtgtcc gcgtatgcaa cgaggtgcag ggtgtttaca acttgagcca
      481 gcaccagcgc ggcttccaga tctgcgtcgc gccagcccac gagggcgaca tgctgaactc
      541 ggtgtgcgtg cagtgcggcc agtgcgcgaa cgtctgcccg acggcggcga tcatcgagca
      601 cagcgccacc gacgaggtgt tcaaggcgct ggaggatcca gccaagcacg tcgtggtcca
      661 gaccgcgccg tccatccgcg cggcgatcgg cgaggggttc ggcttcgagc ccggcacgcc
      721 ggcgacgggc aagatgatca gcgcgctgcg cacgctcggt ttcgaccggg tgttcgacac
      781 gaacttcggc gccgacctga cgatcgtcga ggaggcgcac gagttcatcc ggcgcctgaa
      841 gaacaacgac cgactgccgc tgatcacgtc ctgctcgccg ggctggatca agttcatgga
      901 gcatttttat cccgagctga tcccgcacgc gtcgacgtgc cgctcgccga tgacgatgct
      961 ctccgtgctg ctgaagacgt actacgcgga gaagcagggg ctcgacccga agaacatcta
     1021 cgtggtcggc gtcatgccct gcacggcgaa gaagtacgag gcgcgccggc ccgagcacgc
     1081 ggacgcgaac ggggtgccgt acaccgacgc cgtgctcacg acgcgcgagc tgatctggat
     1141 gatgaagacc ttcgggatcg agttcaagca cctgcacgac ggcgacttcg acaacccgct
     1201 gggcttctcc tccggcgcgg cggacatctt cggcacgacg ggcggcgtga tggaggccgc
     1261 gctgcgtacg gcgtacgaga ccgtcaccgg ccagccgtgc gagaacctgg acttcgtcga
     1321 ggtgcgcggc gtcgagggcg tgaaggagtc gaaggtgaag atcggcgacc agacgctgaa
     1381 cgtggccgtg tccaacggcc tgcagaacgc caagcggctg ttcgaccgcg tgctgaaggg
     1441 ggacaagaag taccacatga tcgaggtcat ggcgtgcccc ggcgggtgca tcgggggcgg
     1501 cggccagccg tacccgccgc cgggcctgca catcatggac gccaagctcc tgcgcatgcg
     1561 ggccaaggcg ctgtacgcga tcgaccacca caagcagatt cggaagtcgc acgagaaccc
     1621 gtacatccag cagctctaca agcactacct cggcgagccg aatagtccca aggcccatga
     1681 actgctgcac acgcattacg aagccaaact gccccggggg acccgatgac agcccaggaa
     1741 gaaaaagtca ccacgctggc cgaactgccc gagcccatcg cgcggttcat cgacgagacg
     1801 cgcgaccggc cgaacgcgga cagctacctc gtggccgtgc tgcagatgat ccagaaacat
     1861 ttcggctacc tgcccgccgg cgcgctgaac accgtgtcgc agaagatgca gatcccggcc
     1921 gcgaaggtga ccggggtggc gacgttctac cacttcttca ccttcgtccc caagggcaag
     1981 cacaccgtgt ccgtgtgcat gggcacggcc tgctacgtca agggtgcggg gatcctggtg
     2041 gaccgcctga aggacctgct cggcatccgg cagatcggcc acgcgtcgaa ggacggccag
     2101 ttcacgctgg aggtcgcccg ctgtgtgggc gcctgcgccc tggcgccggt gctgatcgtg
     2161 gacgacaagg tctacgccaa cgtccggccg gacgaactgc cgaaaatcct gtcgaaatat
     2221 ggtttcaaac cgaaagcgga atagttatat gtaacaggca gggcgcgttg gcgcaacgcg
     2281 ccgtgatgcg aatgagcgac ggcaagacca tcctgatcgt ggacgacgat cccgacttcg
     2341 tggaatccaa ccgggacctc ctcgaggcct acggctactc cgtccacgcc gcgcacaacg
     2401 gcgccgacgg cctggacctg gcgcggaagg tcaggccgga cgtgctgatc ctcgacgtga
     2461 tgatggcgac caacaccgag ggtttcgagg tggcgcgcaa gatccccgag acgcccgagt
     2521 tgaaggggct ccgcgtgctg ctggtcacgg gcgtcacgaa ggacatgcac ctgcctttcg
     2581 gcttcgagcc ggacgcgagt tggcttccgg tggaccgcgt gcttgaaaaa cccatagccc
     2641 ccaaccggct cctgaccgag atccagaagg tgctgggcga ggcgaacccc aaaggagaga
     2701 actcccatgc cggctagcaa gaaaaccatc ctgatcgtgg atgacgatcg cgatttcgtg
     2761 gaatcgaaca aggacctgct cgaggccaac gggtaccgcg tgatttccgc ccacgacggc
     2821 gcgagcggcc tggcgctggc caagaaggag cggccggccc tgatgattct cgacgtcatg
     2881 atggcgacga acacggaggg gttcgaggtc agccggagaa tccccgagtc gcccgagctc
     2941 aagaacatgc cggtcctcat ggtcacgggc atccgcaagg agatgcacct gcccttcgcc
     3001 ttcgagccgg acgagacctg gctgccggta tccagcctgt tcgaaaagcc catcgagccc
     3061 gccacgctgc tggccgagat caagaagcgg atcgggtgac gcgggcggag ccgcgcccgc
     3121 tccgaactac tgcttcgcga gaagggccgc gtaagcgcag cctacgcggg gcgttgtgcg
     3181 gaggatgaac cccgtttcct tcgcgatcct cacttccgcc tcgaaagccg gcccgtccac
     3241 gtgccccttg ggctcggcga tgagcaacgt gccgcccggc ttgagcgccc ggtgaaaagc
     3301 ggccagcagg cgttggacat ccggcacctc gtggatgacc gcgaaggcca ggatgaaatc
     3361 catggcgcct tcgaattcct gcaacccgaa gtcctcgtgc ccgcaacgcc ggatgaagat
     3421 ccggcccgaa agcccgcgcc gggccgcgcg ccgtttcagc tggtccagca tgccgtcctg
     3481 gacatccaca gcgtacaccc ggccggtcgg cccgacgagt tcggccagcg gcagcgtgaa
     3541 gaaccccatg gcgcagccga aatccagcgc gcgcatgccg ggccggacgc agggcgcgag
     3601 gatcttccgg ggatcctgga gcaacctgcg gagcgggttc agcaggagcc agccgatcca
     3661 aacgggacag gcatggtgtt tatgcggggc tgtcatgatg tattactccg ccggttgaag
     3721 ccgcagggaa tccccccgct ccaggctggc cgtgcggccg gccagtcgga tggcgcgccg
     3781 cgcggattcc aggtccgcgc aaacgcgctt gtcgtacagg cggtagctgg cgcggtacga
     3841 atcaggagtc ccgttgacca tgatcacgtt gcaaccgaaa cgcaagccgg cggcgaagcc
     3901 ctcggacgga tcgagcgtgg ccagcgcggt cgtggcgggc aggtgcgtgc cgggcgacgc
     3961 gatgcgggcc agcgccagca ggcggagcac ggtctcccgc tcgccagcct gctggtcggc
     4021 gaacggagtg tccgcctgcg gaaggaacgg gccgatcccg gccatgtcgg gctggaaatc
     4081 ctgcaggaag agtatttcgc ccgccaggtc ctccgcggtc tgacccggca ggccgacgag
     4141 aaaaccgacg cctacctggt agcccagttc gcgcagccgt tcgatgatgc tcatccgcac
     4201 ggcgtgggac tggccgggat gaatgcggtc gtacagcgtg gcgttgacgg tctcgtgctt
     4261 cagcaggtag cgatccgcgc ccgcctcgcg aaaggcctcg tagtcctcca ggggccgctc
     4321 gccgatggac agcgtgatgg ccgcgccggg cgcgtcggtc ttgatgtccc ggatgatgtc
     4381 gcacagcatg gccgtggtgt agtgcgcgtc gtcaccggat tgcaggatga cggtgcggag
     4441 gccccagcgc aggagggtgg acgcggcctg ccggatttcc gcggggctca tgcggtagcg
     4501 cttgaggcgg cgattctcgc ggcgcagccc gcagtaccgg caactccgcg cgcagcaatt
     4561 ggagaactcc acgatgccgc gcaggaacac cccctcgccg caatgctccc gccgcgccgc
     4621 gtccgcccgg cccctcaacg catgcgagtc cggggcccgg agctcatcaa gaatatcgtt
     4681 cttcgacagc atcggaatcc tcaactgcga aacacgcgaa atacgcgaaa gaaaagatga
     4741 ggaactcaat tttcgcgcgt ttcgtagttc cttcttctac aggaacaaat cccgctcccc
     4801 ccgcgccgtc cgttcgtaga actgcatgaa caccggatag cccttcgggt tctgctcccg
     4861 tatctcggcc agttcgtgct gcagcacttt ttcgcccgcc gcctgcgtgg ccgggctggc
     4921 gaagtcgtcc agccactcgc ggaacgtcag gaccgcgttc agcttgcaga acttcccttc
     4981 gcgcccgctg cggagcaggt ccatgatgca ctggcccgtc cgcccgcacc ggtagccggc
     5041 cgtgcagaac gaagtaatat agcccatctc cgcgagttcg cgcaccacgt cgtcgagact
     5101 gcgggtgtcg cccagcagaa actgttggcg cgagccctgt tgcgactcgt aggcttcgct
     5161 gtacgcgccg accccgatgc gcgacgacgc gtcggtctgc gtgcagccga gcgggatcag
     5221 ccgccgccgc atcccggcgt tttcccgcgc ggtcaggatc atgccggtgt acggcacgga
     5281 gagccggagg acgaccaccg cgcgctccat gtcctcgtcg cttacgagga acccggactc
     5341 ctgcgtgaag ggcgtgctct ccgcgggctc gaggcggggg aaggagatcg tgtgcgggcc
     5401 gattccgaac gcgcgctcca actcccgcgc gtgataaaga agccccatga cctcgaagcg
     5461 ccagtcggcc aggccgaaca acgcgccgag accgacgtcg tcgatcccgg cctccatcgc
     5521 ccggtgcatg gcgtacaacc gccaggcgta gtcgcccttg atcgtgccgg cgggatgcag
     5581 ccgggcatac gtgtcgtggt ggtaggtctc ctggaaaacc tggtacgtgc cgagcccgac
     5641 ctcgtgcaga atcttcagtt cctccacccg caggggcggc gcgttgacgt tgatgcgccg
     5701 gatctgcccg aagccgatcc gcgccttgac cctggcgctg taaatcgtgc gcagcgattc
     5761 cgccatgtag tcaatcgcgg aatccggatg ctccccgtaa acgacaatta acctcttgtg
     5821 accgatttgc ccggccagca cctcggcctc gcgccggatc tcgtcctgcg tcagcacgcg
     5881 tcgctcgatc gccgcgttgg cgcagcgcag cccgcagtag aggcagttgt tgacgcaccg
     5941 gttgcttaaa tagagagggg cgaacgtgac gatgcggttg tcgtagacct tccgcttgat
     6001 ccgcgcggcg gccgtcttca tctccgtcca caggtcggga tcggtgacgt gcagcagggc
     6061 ggcggtctcg tccggttcca gggtctggat ggcctcggat ttcgcgatga tggcgcggat
     6121 gcgagcggga tcaggctccc ggttcgcgcg cagccgcgct tcgatctcgg cgtcggggat
     6181 gaaatcgccg tcgtcggtcc ggtacttgtc gatctggtcc cgcgcaatgc ggttgcggat
     6241 ccaggaggag cgttcggtga gttgtgtggt catgagaggg tcggggttca ggacggggct
     6301 cctgcccacg ccgtcttgct ttgcgccttc tgttcaattc cgaatggttc cagcacccgg
     6361 ggcagcacgc cttgcaggtg cgagatgcac aggccgtagt tcgtgatcgg cacgcccgcc
     6421 gcggcggcct tctcgatccg ggccagcacc tcgcgctggg tcagcatgca cgcgccgcac
     6481 aggatgatga gcgagtacga cgccacgtcg tccggccagt cgcggcccga gcagaccgta
     6541 acctccacgt cgaacccgag atgcttcttc agccagcgcg ggatctttac gcggccgatg
     6601 tcgtcctcca cggcgtggtg cgtacaggcc tcggcgatca gcacgcggtc gccgggccgc
     6661 agccggtcga tggccgacac gccctcggtc tgccgcgcga ggtcgccgcg gaaccgggag
     6721 aagaggatcg aaaacgtcgt gcacttcacg ccgggcggcg tctccgcgac catccggtgg
     6781 acgacctggg agtcgcacac cacaagatcc ggcgggcgag ccagttgcga aagcagggcg
     6841 gtgtactcgg tttcccggca gacggcgacc atttcgccgt ggtccagcgc gtcgcggatc
     6901 gcctggacct gcgggagaat gatccgccct ttcggcgcct ccttgtcgat cgggatgatc
     6961 agcacgatca gcccgccggg cgggaccagg tcgccgagca ggctgggcgc cgcgacgaca
     7021 tggtcggggc agatttccag caggtgctcc ttgaactggt gaaccacgcg atcgcgctcc
     7081 gcgcccgtga acgggtgact ggcacactgg agcaggcgcg ggattttcgc gcggaccgct
     7141 tcgataaaag cggccgtcgg ttgttccagg tccgctttat tgatgaccgc gatgaccggc
     7201 gtcttgcgtt tggtgaattc gccgagcagt tcgtcctcga acgccgtcca gcggttcggc
     7261 tccagcagca ggaccgcgac atcgctgcga tccagcgcgc ggcgggtgcg gtcgacccgg
     7321 ccggccccga gctcggacgt gtcgtcgaga ccgccggtgt ccaggaacgc aaccgggccg
     7381 acgggcagca gttccatggc cttctccacg acgtccgtgg tcgtgccggg tatgggcgaa
     7441 gtcagggcga cgtcctggcc tgcgacgagg ttcaggaagc tggacttccc gacgttcgtc
     7501 cggccgaaga ggccgatgtg cagccgcagg gatttgggtg ttttttccat gagcttgatt
     7561 caattgtagg aagccggctt gctggcgact gctatcgcga gcaagctcgc ttcctacttc
     7621 gaggtcatcg ccgatttgac agacacgccc tcgatctggc ccagcttgcc cgtcaggctg
     7681 ccgacctcgt ctgtggtcgc gcggacgatc agggtgatga cgctatggtc ctcttccagg
     7741 tggggaatgc ccatgcggcc gtagatcagg ttgccgaact cggtcagcac ctggttgacc
     7801 agccccgcgc tcttcttgcg gttctccacg acgatcccga cgaatcccag tcttttcttc
     7861 atatgttcct cccgataata aaaaaaccgg cccgcctgag aggcgatgcc ggatgcattt
     7921 tttccgttcc gcccttgaac tcagagtttg cgctcctcgt cgcaggtttc gcgcactgta
     7981 catgccgccg agtccggcgt caaggccgac cgggttttcg tttggcctgc ggggcgggcg
     8041 ggggtagcat gtcccgcatg cggatcgcgt ttctattgca ggacaccggg cgcatttacg
     8101 gggccgagcg ggcgacgctg gacctcgcgc gcggcctgcg gtccgccggc gtcgaggtcg
     8161 ccgcgtggct gatcgaggaa tcgcggctgg ggctggaaaa gagcgagttg aaaaccgcga
     8221 tcctcgaggc gggcctggag tgttccgcgt tgccggttca cagcgccttc tcgcgcgatc
     8281 ttcttcggat gctccgggaa aaagcgggac gcgaaaaacc agatgtcctt catacgatcg
     8341 gcccgaaggc cacggtgcat gcggcgtggg ccttgcgggg ccgggtgccg ctggtgacca
     8401 cggtccacgg ctggctgttc cggcccgatc tgaaagagcg gttttacgag tggctcgaac
     8461 tgcgcgcctt ccggcggttc gaccgggtga tcacgctgtc gcgccattac gagcagtggc
     8521 tggccgcgcg ggtgggggcc gggcgcgtgg tccggattcc atcgggattc gacgcaaggt
     8581 ctgtagccgc ggtcggcgac cgcggccacc agcccatgac gttcggcatg ctcggccggt
     8641 tgagttggga gaagaaccac gagctgttct tccgggccgc gcgccggctc caggatgaca
     8701 aggcgtccgc acgctttctc gtggcgggcg aggggatgga gcggccccgg ttggaggcgt
     8761 tgcgggccgg gctggggctg gaatcggccg tcgagatggc gggatatgta gccagcgagg
     8821 aattcttccg gcaggtccat gtgctggtac aatgctcccg gatggaaaac ctcccgtaca
     8881 gcatcatgga agccatggca cgggagcggc ccgtcctcgc gacgcgcgtc ggcgggctgc
     8941 cggacctcgt ggaggacggc gtcaccgggt tcctggtcga gccgggcgac gacgcggcgc
     9001 tggccgaccg gatgaggaag ttgtccgacg atccggacct ggcgctgcgg atgggaaaag
     9061 ccggccgagc gaaactgcag caagagttcg cgccggaagc ctgcatcaag caacatctgg
     9121 aactatacgg tgaactgacg gcctgatcca tgtccgaacc cctcgtcagc atcatcacca
     9181 gcacctacaa ccggtcgccg atgctgcgcc gcgcggtgga gtcggtgcgc gcgcagacgt
     9241 tcacggattg ggagcacatc atcgtcgggg attgcacgcc ggacgacacg gaagcggtgg
     9301 tggcgtcgtt caacgacccg cggctgcggt ttttcaacct gccggagaaa tccccgccgg
     9361 gcagccacgg ggcgatcgcg aagaaccacg gcatccagcg gatggcgcgg ggcgggtata
     9421 tcgcgtacct ggacgacgac gaccgttacc gcccgctttt cctggaggtc atgatgggct
     9481 acctgcgggt gcatccggac gccgcgctcg cgtactgccg gtcgatgtac cgggacaagg
     9541 acaccggcca ccgcttgtgg ggcaacccgt tccagcgctg gctgcacggc tactcgaagg
     9601 agaagctgcg ccgctacaac ttcctgaaca cgaactgcgt catccacaag aagagcgtcg
     9661 cggaggaggt gggcgggtgg gatcccggct actactttga cgattacgag ttgtggctgc
     9721 ggatcagcga gcggcacgat tttcactacg tcaaccgcgt gctggtggaa accttcgtga
     9781 gcgagccgtc gttcctggtc cgggcggtca cgaagggctg gcggatcctg cggcacggcc
     9841 ggacgacgcc ggtgaactga cgccgcgcgt tggtcttgac aggggccgaa caatggtcat
     9901 cctatgacgg tgccggaagg ggaaccacgg acatgatcgg cgggaaaaaa ataacggtgg
     9961 tcatgcccgc gtacaacgcg gcccaaaccc tcgagaagac cgtccaggcc ctggaccgcc
    10021 aggtggtgga cgatatcatc gtggtggacg actgcagctc cgacgagacg gtcgcgcggg
    10081 cgaaggaact cgggttgttc gtccaccgcc acgagcgcaa cctcggctac ggcggcaacc
    10141 agaagagctg ctatcgcctc gcgctccagc gcggcgcgga catcatcgtc atggtgcacc
    10201 cggactacca gtacgacccg cgcctcgtgc cggcgatggc ctcgatgatc gcctcgggcc
    10261 tgtacgacgc ggtcctgggc tcgcggattc tctgcggcgg caccctggcc ggcggcatgc
    10321 cgtactacaa gtacatggcc aaccggctgc tgaccgcgtt ccagaacatc ctgatcgggc
    10381 agaagctgtc ggagtaccac acggggttcc gggcgttctc gcgcaaggtg ctggagacgc
    10441 tgccgctgga ggaaaactcg aacgacttcg tgttcgacaa ccagatgctg tgtcaggccg
    10501 tcgcttttcg tttccggctg ggcgaaattt catgcccgac gaaatacttt gccgaggcct
    10561 cctcgatcag cttccgccgg tccgtgacct acgggctcgg cgtgttgcgc gcgaccatgg
    10621 ccctcgccct gcatcagcgc ggctgggcga agaacgagcg cctgttcggg gagcagggga
    10681 gacggctgac cccatgagcg aggaagccag cggcgcgccc gggtgcccgg cttgcggggc
    10741 ggcatggggt aatgaagtca tggcgtgcac ggccagcgac ggccgggagt tccgctacgg
    10801 ccggtgcccc gggtgcgggt tgtatcggct cacgaccctg cccggcccgg cggagatcgc
    10861 cggattctac gacgccgcgt attacggggc cggggaacgg aagttcccag cgctcatgga
    10921 ccaggtgcgc aatctcttcc tccgccggcg cgcaacccgg gtcgcgggct gggcgggccg
    10981 cgcggcaggt cgcgcgctgg acgtggggtg cggcgacggg cggttcctcg gacatatgca
    11041 gggattgggc tggcaggtcg ccggcacgga actgccgggc gcggccttcg accgggcgca
    11101 gaaaatcccc ggcatccagc tgaaggcggg caccctgacg ggcgggctct ttccgccggc
    11161 ctcgttcgac gcgatcacca tctggcacgt gctggagcac gtgccggacc cgctggcgat
    11221 gctgcgcgcc tgccgggaac tgctcgcgcc cggcgggctg ctggtcgtgg aagtgccgaa
    11281 caccgacagc tggcagaacc agttggccgg tccgctggcg ttcaacgtgg atccgccgcg
    11341 ccacctgtac cagttcggcc ccgagtccct gggccggctg ctcgcgcggg ccgggttctc
    11401 catccggcgt cgcgaaacat cgtcgctgga aatgggcgtc atcggggcgg cgcagtccgt
    11461 gatgaacggg ctgatcaagc cgcgggacct gttctacgac ctgctgcggt cccgcgggaa
    11521 atgcccgggg gctccggcgg cgaaatggat atccctgctg ctggccgtgc cgctggttcc
    11581 cgcggggctg ctcttcaccg tggccgaaac cctggcggga cacgggccgg cgttgaggct
    11641 gatttgcgaa ggcgccgcga attcgtccgg agggagttga cgtggcgaat ttccagccca
    11701 tgaaagatcg gttcctcgtg gtgctgggcg agaacctgcg gctctgcggc gcgggcgggc
    11761 gcttcctgga ttacggatgc ggccggggcg acgtggcgga gtacctgatc cgcggcgggg
    11821 gtttcacgga ggggctggcc tacgatccct cggtgacggc cgggcaggaa agctcctcgg
    11881 ggccgcgcga gggcggccgg caactggcct atcgccggac gcttccggag gcgcgcggcg
    11941 acttcgacgt ggcggtgctc ttcgatgtca tcgagcacgt gcccgagccg gcgcgggtcc
    12001 tcgaggatat ccgggcccag gtcaagcccg gcggctggct ggcggtcacg gtgccctaca
    12061 atccccacga gtggggcgtg gacgacgatt tctacggcca cctgcgccgc ttgagcatgc
    12121 gcggtgtcgt gtccatgctc gaggccggcg gctggggcgt gatccgggcg ctcgacccgt
    12181 cgtttccctc cttctggttc atccgccgcg tctacctgat gacccggcgt ttcctgcggc
    12241 agcccatccc cgccggcgcc gacgtggcgg gcacggacat gcatcgctcg ctggcctcct
    12301 cccggcagag cgcatgggac accggggggc cggtccagcg catgttgtcc ggcgggctcc
    12361 tgccctggaa actgatccgc cgcttcgatc tctacttcga gtccatcttc cggggcttcg
    12421 agctgtttat cctgtgccgg aaacgcgagg gcgaggccag ctgcggcgtc tgcggctgcg
    12481 gccgttttgt gtaccaccgg tttttccacc gctacagcct gcagcgctgc acgtactgcg
    12541 agacggagaa gatcctggcg gcggacagcc gggaggcggg gtcggacggc ttgcggccgt
    12601 cgccggtcca cggactgatc cgccggtggc gcgcgcggcg cgttcgccgt cttgccggcc
    12661 gggcctccgg cgccatggtc gcggtggtgc ggaatccggc ggaggatatt gacctggccg
    12721 aactccaggc cgggggccgc gaggtccggt gtctgggctt gcccgagtgg ctggcggcgc
    12781 cggcgcaatc gtgcgcggtc gtagcccttt ttcacgtcat tgaactggcg cccgaggtgg
    12841 acatggtgct ggaccgattg gatgcagcag tggcgcacgg cggccacgtg ctgctcgagt
    12901 acccgaacag cgggtcgtgg ctcaagcggg cgttccggtg gcgctggttc ggttacgatc
    12961 cgccgaacca ccggcatatt gtcgattccc ggaaactgtg cgaccggatg gggttgcgaa
    13021 attaccacct ggtccgggag cggcatttcg cgccggaata ctccttcttc atcttcatcc
    13081 agacgctgat aaacgtcctg ctttcgttcc agcgggatgc gttgtaccgt tggctgcggg
    13141 gcgtcggcac gtccccggtg gaccgggcgg cagcctggct gtccattccg ctggcggtgc
    13201 tgctggcgcc gctgttcgtc atctaccagc cgctggcctc gtggttccgc cggggctgcg
    13261 tggtgcagca ggtgtaccgg aggaccgaca tcgcgtccgc atcgcaaggc gggggtgcgt
    13321 aagaaatgga tcccgagatt ccagtgcgga gcggcggggg cgtggcctgg ttggaccggc
    13381 attacctgtt tctcgtcacg gtcctgctgc tgctcgtgta catgttcacg tacttcgggc
    13441 tgaaccagct tcccatccga gacgacgggg cggggtatta tgcgtacctg ccgtcgctgc
    13501 tgctctacgg cgacccgagt ttcgaaaccg acgcgcgggc gcactacggc ggggaattcc
    13561 cgcgctggac ctacgtgcgg cgttatccgc cgaccgggcg ttacctgaac tcgctgaaca
    13621 tcggcgtgtc cgtgatgacc gcgccgttct tcgtcgcggg gcacgcgctg acgtactggt
    13681 tcggctggcc gtggaagggg gggtggccgt ggatggagat gcggtacccg ccggacgggt
    13741 attcgatgtt ctaccagcac gccgcggggc tggccgggct cttttactcg ctcatcggcc
    13801 tggtgctgtg gaagcggctg ctggcccggc ggttttccaa gggtgtcgtg ctggcgacga
    13861 tgaccgcgct cctgctcgga acgaacctgc tcaattacgt cgcggcggag acggtgcacg
    13921 cccattcgtt cacgttcttc ctcgccgccg cgctgatgac cctgacggag gcctggtacg
    13981 aacggccggg tcggctccag gtttccctcg cgctcggcgc ggtggccgcg ctgctgttcc
    14041 tggtccgcca gcttaacgtc ctggtgctgc tgtgggtgcc cctctacggg atcgtcggcc
    14101 ggcgggatgt cggcgcgcgc gcggtgttct tcctgaagaa tgggcgggac atcgcggtga
    14161 tgggcctgac cgccttcgcg gtcatgatcc cgcagttcct gatgtggaaa tactcctcgg
    14221 gtcatttcat cgtaaaagcc taccagcaca tgggggcgtc cagtttcggc tcgccgcagg
    14281 tgctggatgt cctgttcgga gtgtacaagg gcctgtttgt ctggtttccg atcttcgtgc
    14341 cggcggttct cgggattctt tgcctgaagg ggccggccgc cgcgtaccgc ctgcccgtga
    14401 tcgtgatctt cgcggtctac atcctggcca tatccagcct gaagatatgg acttcggccg
    14461 gcgggttcgg caaccggtac gtggtggaca cggtgattct catgggcttt ccgctggcgg
    14521 ctttcttcag ttccttgagc tcgcgctgga cccggcggac cgtcgccgtc atcgtgctcc
    14581 tgtgtatttt ccaggcgttg tttttgctga cgcttctgta tcgccgggag atgacgttct
    14641 acgggctgga cgcgcagggc tttttcgacg tcttctggtg gcgcaaggag tcgctggtaa
    14701 actggttgcg aaccaggttc taggccgtct cccgtttttg caaaacggcg gttaaagcaa
    14761 tttacaccaa cgcttaaccg ggtattatat attaaggacg tgaggcgtat caggttgtgg
    14821 ggcgggccgg gttctgcggc cggggcacag gcgggcggaa gcgttgtctg caacgacgaa
    14881 tcaaggcttt acgaagacgg acagaggaag gcggatagca ctgtgaacta ccatcacttt
    14941 acccggcgcc ggtggaatag catgggatgg cccacgctgc tgttgattcc ccttttcatg
    15001 gcggccggca ccgcgccggc ggacgccgcc acgtactacg tgaatgacgc ctccacgaac
    15061 ggcgacgtgt attgcagcaa catgggcagc gacgccaatc ccggcaccaa cgccgccgcg
    15121 cccaagctga cgctgaccaa cctgttcgcg agcgtgacgc tgctgcccgg cgacatggtc
    15181 tacatcgaca ccggcggcta ttcgaattac acggtcaacg tgccggcctc ggggacggcg
    15241 ggcaacctga tcacgttcca gggcagcacc aactacgggg cgggcggaac ggtgttccgc
    15301 cagaacaacg ccgcctccaa cgtgttcagc ctggcgggcc gctcgtacat cggcctgcgc
    15361 gacctgaccg tgcgggacgg acagtgcggc gtgtacatga ccgggtcgtt ctgcgatttc
    15421 caccggatca ccgccatccg gaactcacgc ggattctggt cgcagagcgg cggcgtgaac
    15481 gccatgcgct actgcgtggc ggcggagaac gtcatcggcg tgtcggggtt cgacacgacc
    15541 acgttcaacc agggcgtgct gtggagcaac accacggcca tttcgaccta cacgagctcc
    15601 tcgatcgcgt tgagcaacag cgtcatcgtc ggcggcatcg ccttcacgct gctgcccgag
    15661 gcggcggagt ataacgtgta ctggcgcacg gatatgagca gcaacaccgt gccctcggtc
    15721 cggacgctgt cggagatgca ggaggccatg ggacactcgt tccgcagcat cgtggcggac
    15781 ccccagttcg gggatcccgc gggctacgac ttccatccgc gcagcgtgac gggccggtac
    15841 gtgcaggcga ccaagacctg gacgaccgac gccgtgcact cggtgctgat cgacttcggc
    15901 aacccggcct ccgcgtacgt caacgagccc gcgcccaacg ggagcaacat caacgccggg
    15961 gtctggggga acacggcgga ggccagcaag agccggacga acgcgtggct cctggcggtc
    16021 agtttcaacg acggcggcac gctgcagcag acaggccgcc tgtactggac ggccggcaac
    16081 attccgacca ccgagaaggt gcggctggac tattcccgcg acagcaaagc cacgtggcgg
    16141 atgatcgcga ccaacgtggc ggttacgaac ctgtactacg cgtgggacgc ctcggctgtc
    16201 acgtccggcg ccgtgatgta ctggcgggtg gcccgggaga ccaacaccaa cttctttgac
    16261 gtctcggaca cgaatttcgc tttgcgcggt ctgacgccgg tcccgtacta cgtcaacgat
    16321 accggcacgt ggggcgacgt gtacacgcat ttccccgggt cgtcggccaa cgacgggctc
    16381 acgcccgaca cgccgaagga caccatccag gggatcctcg acgcctattc gctgggcggg
    16441 gaccacacta tctacgtgga cacggggtac tacacgggcc agaccgtggt cgtcaccacc
    16501 tcggattccg gctggtcgaa ccggtacatg acgatccagg gcagcactaa ttacttgtac
    16561 gacgggaccg tgctgaaccg ggggagtgcc gcggcggacg tggtgatcct ctcggccgtc
    16621 cggcgggtga agctccgaga tctcacgctc ctgaacggcc gctacggcgt gtttgccagc
    16681 agcagcctgg actgcgagta cgagcggctg acggcccggt tcaacgacac gggcttccgc
    16741 ggctcctcgg gcaacaacca tgtcttccgg cgctgtgcgg cggcccagaa cacgacgggc
    16801 gccgccgggt tcaacctttc cgtgctgtgg gactacggcg tgagctggag caacacgacg
    16861 gccttcgcgc cgtccgtccc ctcggcctgg agcgtgagca acagcgtgat cgtcggcggg
    16921 acggccttcg gcggggcggc ggcgcccggc gggggcgact acaacctgtt ctggaacgcg
    16981 gcgattcact ccagctacac gagcctgtcc gcgctccaga agggcgccaa cggctggtgg
    17041 aactgcgcct accgggagcc gttcttcgtc gacccgacga acgcggactt ccacccccag
    17101 agcatggtca acacctactc gaacgggacg tgggcgaagt acgccaacca cagcgcctgc
    17161 atcgacctgg gcgacccggc ggggtcctat gccctggagc cctggccgca cgggacgaac
    17221 gtcaacctcg gcatttacgg caacacggcg gaggccagcc agagccgcac gaacgcgtgg
    17281 ctgatggcca tgaattacaa cgacggcggc gtgctcaacg cccagctcgg ggagcgcgtg
    17341 tactggaagt tcggagacat ctccacgacc aaccgggtgc ggatcgagct ctcgcgggac
    17401 ggcggggcga cctgggtggt ggcggggtcc aacatcctgg cctccatcgg gtactgggac
    17461 tgggccaaca cgaacttcgc ctcggcgcgc ctcgcccgct ggcgggtggt gctggaatcc
    17521 aacacgaaca tcttctccgc gacctcgtac acgaacttca cgttccagaa tgggcgctac
    17581 gagtactacc tgaacgacga gaatatcacg aacgatgtct actgcaccgg gccgggcagc
    17641 gacaccaaca cgggcacctc ggccgggagc ccgaagtaca gcctcaaatc gctcgtggac
    17701 gcgcacgaga tccagcccgg cgacatcatt tattacgata ccgggctcta tcactactcg
    17761 gccgaccagg ccgtcagcag cctggacacg ggcgtcagca acctgccggt cacgatccag
    17821 ggcagcacga actgggcggc gggcgggacc gtgatcgagc ggcgcacggg gccctacggc
    17881 ctcgacttga gcggcgccgc gtactactgg atcgagaatc tcaccgtcac caacgcgcag
    17941 acggccgtcc ggatccaggg caccaacggg ctgcgcctgg tccgcgtgag cgtcaagcgc
    18001 agcggcaacg ggtttgaggt ggccagcgcg gccgacgtgg tgctggagca ttgcatcgcg
    18061 gcggataaca cgggcaacgg gcttctcgtc agcggcgcgg gcagcgacgt ccagtacatc
    18121 cgcggggtgc attggcagaa ccagggcgcc gccgtgcgcg tggccgccgg gcgcgtggcc
    18181 gtgagcaaca cggcgatcgt cgtgtcgcac ccgacggcgt tcgggtacca cgcggccacc
    18241 ccgaccaaca tcgttggcga ttacaacgcc ctgcacgtgg agagcaacgg cgtggcggcc
    18301 tatctgacct ccctgggccg gaacctggac tcgctggacg cctggtcggt ggagaccggg
    18361 caggagcgca tgagcctgcg gaccgacccg ctgttcgcga acccgtttta cggggatttc
    18421 cacctgaaga ccgaccagcg cgacagccgg tggatcacgg gctcgggctg gtatcccggc
    18481 tgggatgcgc agacctcgcc gctcatcgac gcgggcgatc ccgcgagcgg cttcaccaac
    18541 gagctggcct acaacggcgg ccgcatcaac atcggccggt acggcaacac ggcggaggcc
    18601 agcaagcgcg gcatgagcaa ccggctgtac gcggccagcc tgcggcaggg cgggtgggtg
    18661 cggggcacca gcacgctcca ctgggtcgtg ggcggcgccg cgaccggcca cttcgtcaag
    18721 atcgagttct cgcccgacgg cggcgcgtcg tggcttaccc tgaccaaccg gatcgccgcc
    18781 tcggccgagt ccttcgcgtg gaatacgtgg gcggtgacga acacgccggc tggattgtgg
    18841 cgcgtgacca gcaccaacac gcccgcgctg ttcgaccagc cgaccaactt cttcgcggtc
    18901 cgcaacgcct cgctttccgt gtacatcaac gacgcgtcca ccaacgggga cgtgtacgcc
    18961 gccgcggtcg gcgggccgac gaactggcag gcgaccgccg cccggccgct gaattcgctg
    19021 ccgcgagcgc tgacggcgtt cgacctggag ccgggcgaca ccgtctacgt ggacacgggc
    19081 gcgtacacca acagcgagga cacggtcttc ctccggcggc acaacggctc cagcgcgcag
    19141 cccctgctgg tgctcgggag cacgaacgcc gccgcgggcg ggtcggtgct gacccgcgcg
    19201 ggcgacggcg cgaccgtcaa caacctccgc ttcgactacg cgcagttcgt gatcgtatcc
    19261 aatctggcgc tacgcggcgg gcggaccggc gtgcggatga acggcggcgg ccaggtccag
    19321 ttgagccggg tcgacgtcag cgggtgcgtc agcaacggcg tcgaggtgct gaactccacg
    19381 aacgttctcc tgtctcgcgt ggcggcctac aacaatcccg gctacggcct gaacgtattc
    19441 atggccaacg gcgtccagtg gctgcagtcc gtcctctggt cgaacgccgc cggagcggcg
    19501 aagattgtga acggcagcct cgtggtcacc aacagcgtgc ttgaagcggc cgtggacggc
    19561 acctatgtct tcacgctggc atccaacgcc gtcgtgcggt ccgacttcaa cgacatcctg
    19621 gcccggaacg gcgcctatcc cgcgctggac ggcttctatg tctacaaaaa cttaagccgg
    19681 tggaaagacc ggaccaccaa cgacatccac agcctcacgc acgatccgct gttcgtggac
    19741 gcggccaacg gcgatttcca cgccttcagc caggccgggc gcttcaaccc gacctcgctc
    19801 accttcgtgg ccgactccac caactccccg ctgatcgaca cggggccgac ggactggccc
    19861 tttgccgccg agacgggcac gaacggatac cgggtcaacc tggggctgta cggccatcac
    19921 gggcaggcca gccggagccg gaccaacgga tggctcctgg cgctgaccct gaacgacggc
    19981 ggcagcatcc gcgggaccgg cacgatctac tgggtggcgg gcggggtgac gacgggccag
    20041 cagatcacga tcgattactc gtgggatgcg ggcgagaact ggaccaacat cgcgaccaac
    20101 gtggcggcct ccgcggggaa catcccgtgg gcgaccgacc gggtgcgggc caccagccag
    20161 ggcgtgctgc gctaccggag ccagccggac gcctccgtcg aggcgatgac ggagaggttg
    20221 ttcagcatca acaacacctc gctgaccttt tacgtcaacg acgcctacac caacggcgac
    20281 gtctatacca cggccccggg gcagaccaac tacgacggct tgaccgagga ctacccggcg
    20341 gattccatcc tgacgatcct gcggcgctac gatccgcagc ccggcgaccg gttcctggtg
    20401 gacagcggga cctacgtcat gccgagctcg gtgaccatcg acagctcgac gatgggatcc
    20461 ctgaccaacc tggtgctgtt ccagggcagc accaaccagg cggtgggagg cacggtgctg
    20521 gacttcggcg ggcaggtgta cggattcagc atcgagaaca cggaaggcat caacctgcgg
    20581 catctgcgca tccagaacgc gcggagcggc gtccggctcc gcgtggtcac caactgcttc
    20641 gtcgagtcgg tctgggtgcg gggcgggggg catggattcg agatcgaggg cagccggaat
    20701 gtcgagttga cccgctgcgc ggccgtcggg gcggcgacaa gcggcgtgta taatgtgttt
    20761 tcatccaata cgatatggcg caacggagtc ctctggtcca atgcgatcgg cgtgctgctg
    20821 gcgaacgtca gcgccgtgcc gaatccgccg gcgaactaca tggtcttctc caacagcgtg
    20881 gtgggcgcgt tcggcacctc cgcggtggcg tacacgatca acagcggcgt gctgctggcg
    20941 gattataatg gcctccatct ggcggaaggg gcgcgaatgg cccggtcgta ccgcgtgccc
    21001 ttcgaggtgc tttccgacgg cgtggcgcgc tggcgcgcct ggtccacgca ggacctgaac
    21061 agcgtggccg gcgacccggc cctgtgcgat ccgggggcga tggacttcca cccgttgagc
    21121 ccgcggggac gctacagcgt gctgggcaag ggattcatcg ccaccgacac ggtggcatcg
    21181 atcctgatcg acgtcgggcc gaccaacgcc accgactggg ccagcgagtc gagcccgaac
    21241 gggtcgcgcg tgaacatcgg catgtacggc gggaccgtcg aggcgagccg cacgccgacc
    21301 aactcgttcc tcggggtgat cagcctgaat gacgggggcg tggcgtccgg gaccaatttc
    21361 ccgctccgct ggatggcccg gggcaacgcg acgggccacc tggttcggct ggatttctcc
    21421 tggaactacg ggacgaactg gcagacgatc gtcacgaact ggccggcccg cgccgggacc
    21481 tatacgtgga acaccaccaa tttcacgtcc accctgttcg gcctgtggat ggtttcggac
    21541 gagacggaac tggggctggc gtcggtctcc gcgcaaccct ttgccctccg gaacaccaac
    21601 ttcctcttct acgtcaacga caccgggact gtgggcgacg tgtattgcga cgccgcgggc
    21661 gccgagggcc agacgggccg gggcacgaat gtcccgatgc tttcgatcca gcgggtcctg
    21721 gacgcgtacg acctcgaggg cggggacatc gtgtacgtgg acacgggggt ctacacggga
    21781 caggacgcgc ggctgaccgt gtcgcagttg gacaccgcgc ctcccggcgg ggatacccgc
    21841 ctgtgcctgg tgggcagcac caacgcgctg gcgggcgggt cggtgctgga cggggcggcc
    21901 gggctgttcg ggctgaacat gcaggaagcg tacggcgtgg acgcgcggga cttctcgatc
    21961 cagaacatgg ccagcgccgc cgtgcgggtc gcccagtccg ggcagtgcgc gctgtacaac
    22021 gtgcagaccc gggacggggc ggtcggcttc gacctgcagc ggtcgatcga cagcctgttg
    22081 agccagtgct cctccgtcgg ctcggatacc ggcctgcgca atctctacgc gacgaacacg
    22141 gtgttgcaga actgcgtgct gtggtcgaac cggctcgccg tcagcctcgg gtcctatgcc
    22201 ggcgcggcca tgcgcaacgg ggttgtcgtg tccaacagcg tgctgggcct gttcggggcc
    22261 cagcggtttg cctacgaggg cgagaccgcg tccctgcagg ccgattacaa caacgtgtac
    22321 ctccagggcg gggcgtacgc ggcgcggcgt ccaggcctta ccttctccga gacgatcgac
    22381 accgtgtccc gctgggtggc gcaggcgggg caggatcgcc acagcttgag ccacgatccc
    22441 ggcttcgcgc aggacggcgt ggatttccac ccgaagagcg cgcgggggcg ttgggcggtg
    22501 accaactacg tgtatacgga tgtgaccaac tcgatcctgc tggacgccgg cgcgccgggg
    22561 acgaattact tcaacgagcc ttcgcccaac gggagccgcc tgaacgtcgg cccctacggc
    22621 aacacgacgc aggccagcct ctcgcccacg aacgcccggc tgacggtggt gagcctcaac
    22681 gacggcggcc gcgccctggg cacgaaccag tacctgtact gggtggcctc gggcgacgcg
    22741 acggggcata cggtgaagat ctcctattcc ggggacggcg gcgccacgtg gacgacgctg
    22801 gtctccggcg tgtccgcgct cgccagcaac tacctctggg acaccacgac gtggccctcg
    22861 accctgctgg gcatgtggga ggtccggagc cagaccgaca cgaacatctt cggccgcaac
    22921 tcgacggtgt tctcggtgcg caacgaaccg atgcggttct acgtcaacga cgccaacacc
    22981 aacttcgacg tgtactgcac ggccgctgga cattccacca acaacggcct gttcgcgcat
    23041 acccccaa
//
LOCUS       DAOOTK010000040        22954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1550_length_22954_cov_6.480763, whole genome shotgun sequence.
ACCESSION   DAOOTK010000040 DAOOTK010000000
VERSION     DAOOTK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22954
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_22954_cov_6.480763"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PK634_06475"
     CDS             <1..563
                     /locus_tag="PK634_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOW97418.1"
                     /translation="YDREKLQERLAKLAGGVAVINVGAATETEMKEKKARVEDALHAT
                     RAAVEEGIVPGGGVALLRCQPALADVKLDGDAGIGVDIVRKALEAPLRQLTANAGVDG
                     SIVIQEVKKQKGSYGYDVAKDEYVDLVKTGIIDPTKVARSALQNSASIASLLLTTECM
                     VAEIPEKEKKPMPGAGGGMGGMEDMY"
     gene            648..890
                     /locus_tag="PK634_06480"
     CDS             648..890
                     /locus_tag="PK634_06480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOW97419.1"
                     /translation="MNVKYMELAKEKVPNIPLLINMVSRRVRQLNRGERPLVKPDNMQ
                     MSPMNIALKEIAEGKLSAEISFVPVRKTVESNPLSL"
     gene            918..1409
                     /gene="smpB"
                     /locus_tag="PK634_06485"
     CDS             918..1409
                     /gene="smpB"
                     /locus_tag="PK634_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOW97420.1"
                     /translation="MAPAPSPARPAVKTMATHRKAFRDYHILEKIEAGIELRGAEVKS
                     IREGRFSLTESHALVDGGQVMLMGLHVQPFTCSRVEEHDPLRPKRLLLHRREIDRLAG
                     QTAVKGCALIPLRVYFKKGLVKVELALGKGKQSEDKRETLKRRTAEREAERAIASRTR
                     RRG"
     gene            1423..2091
                     /gene="nfi"
                     /locus_tag="PK634_06490"
     CDS             1423..2091
                     /gene="nfi"
                     /locus_tag="PK634_06490"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066291.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOW97421.1"
                     /translation="MPAPIRHSWNLAPAQAIALQGKLAARVSIRPLPSGIRWIAGLDA
                     AFSEDECVAAVVLWDTRRKEVVEQRIARAPLRFPYIPGLLSFREAPALLAALEKLERR
                     PDVLMCDGQGLAHPRRFGIACHIGVLAEIPSVGCAKSLLVGEFREPAKRRGSRAALAD
                     RGERIGTVLCTRDGVRPVIVSVGHLANLATAEKLVLRCAVSYRLPEPTRLADQLCGAT
                     AHLV"
     gene            complement(2237..3103)
                     /gene="rsmA"
                     /locus_tag="PK634_06495"
     CDS             complement(2237..3103)
                     /gene="rsmA"
                     /locus_tag="PK634_06495"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOW97422.1"
                     /translation="MNLTHPSEVKELLSRLGLRPRRTLGQNFLIDANILRILLRTAEL
                     HREEQVLEVGPGLGVLTEWLVRYAGRVVAVEKDPVLFGFLKTHFAEAPHLELIHADIL
                     DVDLETLLAGGISKVVANLPYSVGSRFLVDLFQTAHPPTRIVVTVQREVARRLAAKPR
                     AEDYGLLSILAQTAFAVNIVKEISPTCFLPPPEVRSAIVELVRLPDDREPRSRNCAAL
                     LKLLQDAFARRRKQLAPFLKARAGLTDSGTGILLDRLGIAPRARPEDISPEQWVLLSN
                     ALAPESDSQARH"
     gene            complement(3186..4157)
                     /gene="pdxA"
                     /locus_tag="PK634_06500"
     CDS             complement(3186..4157)
                     /gene="pdxA"
                     /locus_tag="PK634_06500"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOW97423.1"
                     /translation="MSAMLRIGITLGDVNGIGPEIALRAVARGGWPANVRFVLVGSDA
                     LVRAQARALRLRLPGDRVSVWDPAPALAPDWKPGRIRADASRAAGAWIRAAAAACLDG
                     RLDAMVTAPICKEGFQKAGIHVPGHTELLAELTGTRRYAMMLFGGPLRVVLATRHLPL
                     ADVARKLKRADVLEAIRITGEALPWLGCRGGRIGVCGLNPHAGDGGAIGREEITVIAP
                     AIRAARREGFNAVGPVPADVIFRQAVRGDFDAVVAMYHDQGLGPLKMLAFETGVNVTL
                     GLPIVRTSPDHGTAFDIAGRGRANPSSIVEAIRQAIALTRRRNPWKK"
     gene            complement(4181..5152)
                     /locus_tag="PK634_06505"
     CDS             complement(4181..5152)
                     /locus_tag="PK634_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOW97424.1"
                     /translation="MKRMRGMLGRMAAAWLAWAASAGARSVAVDGYAAMVNQRVILRS
                     EVFAMVYPLQQKLKLEHSGAELEKKLEEAYTNVLESLIERALIVEEFNRQGHQLPDRA
                     IDTQVNSLISERFNNNRMAFLDALAEERLTLAEWREQAKDGLIVTILRRTEILDRVAV
                     APRQVREYYEKNLDRYRTPEEAQLRLIVLHPGATPEERAAKIEEARRIRERLAGGEDF
                     ATVAKTASEGPKAAQGGDMGWMQTSELRAELAAGVKDLEPGQLSEVIEASDEIYLAKV
                     EGRKQAAVTSFQDARQEIEEQLHKQEMDRLYDAWIARLKNRFYVKVF"
     gene            5279..6946
                     /gene="ilvD"
                     /locus_tag="PK634_06510"
     CDS             5279..6946
                     /gene="ilvD"
                     /locus_tag="PK634_06510"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOW97425.1"
                     /translation="MRSDKVKTGFERAPHRSLLRATGLTTEAMSKPFIAICNSYTDII
                     PGHVHLRKVGDLVRKAILAAGGTPFEFNTIGICDGIAMGHAGMKYSLPSRELVADCVE
                     TMVNAHLFDGMVCIPNCDKIVPGMVMGALRCNIPTLFVSGGPMRAGKMPDGRAVDLIS
                     VFEGIGAFKAGHISEEQLEEISCHACPGAGSCSGMFTANSMNCLCEAIGLALPGNGTI
                     LAEDPKRRSLWRAAGKRIVEMVREDLKPRDIVTKESLDNAFVLDMAMGGSTNTVLHTL
                     AIAREAGVDYRLDRINELSKKTPNICKVSPSSPYHVEDVDRAGGISAILREISRVRGL
                     LNLDCRTVTGKTLGKLIARAKSKDPACIRPVENAYSPEGGLAILYGNLAPEGSVIKTA
                     GVDPKMLMHEGPAIIFESQEDACEGILAGKVKPGHVVVIRCEGPKGGPGMQEMLSPTS
                     YIMGQGLGDKVALITDGRFSGGTRGACIGHVSPEAAEGGPIGLLKDGDLISIDIPARR
                     LDVKLSDGELAARRQSWRAPEPRFKTGWLARYIKFATSASTGGALKE"
     gene            complement(6956..7348)
                     /locus_tag="PK634_06515"
     CDS             complement(6956..7348)
                     /locus_tag="PK634_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOW97426.1"
                     /translation="MKAPAQATNSPAGGWSAMLRARMIHNAAVRSQPAAQHGELRIEV
                     PTRRPGWLVPPLSWVVRPPAFRTLTLDALGVEVWTLCDGQRTVEAVIDSFAEKNRLTF
                     HEARVSVTAYLKSLLQQGALAVAADKNP"
     gene            complement(7345..8244)
                     /locus_tag="PK634_06520"
     CDS             complement(7345..8244)
                     /locus_tag="PK634_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97427.1"
                     /translation="MLHPMNADHGPAGQTWIWRHFRLELPADWEMLQFSRDMEKGRCA
                     FADRDRFRLEFDWRKVPGAPDFERMMGDYRARLESSSGAAVSARSAGAWHGLQVRRPH
                     GSSSRFGAYFAENGCLVELVFLWPGEPEDPLERSILDGFAAEPETKAGGRRWRAFGLD
                     CVAPASMKLAQCVAQPALAGLEFAGSRAPDRWSFRRLGLVPHWMKTSVGEWLDAQTAG
                     RVQEHQRSSSDKAGHRMEKVEGHFIPRGLLQRRGRYEAAAWICPRDGRLYHAERIRPR
                     GDDTNAEPPDGYIACCPGVRWPP"
     gene            complement(8290..9570)
                     /locus_tag="PK634_06525"
     CDS             complement(8290..9570)
                     /locus_tag="PK634_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97428.1"
                     /translation="MSRGAFIALLGLALAARAPAVDPPIYVHIVMHNEEGGDYHTNPP
                     AFLRERSNLVVFASMLSSHGVMFNWQSDWSFLRAVTNFDHGTPETGGANVVAWLKNMG
                     FEVDPHAHESDEGQHSYADVAFLMGQCGVPPSDIAGGFIAWPPTSSVLAHLSGTVTGL
                     QYTAETWMPAVLWGGGTASHRDETNLWMSGIWRPKDTNYFTRHDDAAPVPHIGNFGGK
                     QWRWLNLDRLVELRGRGLLQTNRIYTCAIFAGQSELLPPFIVEFETNLLLRMTNSDLV
                     WAGLKQTYEAWQTAYGSEPNLFPFDGSLDSDADLMADGWEASNLWDLIASDGTNDGDG
                     DSMSDLGEFITGTHPADTSSCFAAGIHAVTGATCAISWSARSGNTYYLTSTTNLLAGR
                     WLTNHVSAGIDGGQAHTDTPPVGAVMYRVRVTRP"
     gene            9662..10423
                     /locus_tag="PK634_06530"
     CDS             9662..10423
                     /locus_tag="PK634_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOW97429.1"
                     /translation="MAQGPYDCLYLSPHLDDAILSCAGQISLRTREGRSVLIYTAMAA
                     EQVPSPLQANGARFLKLCGLGPDAGRVRRAEDARACARVGAKPDHGDLPEAVYRCDAS
                     GRELYPTHESLFEAIRPEDEGLMDRLARSFGALPRAAAVFAPLGAGGHVDHVLVRRAA
                     EQVFGAGLSYYEDYPYAVCRDVVRRLTQPRRAWSTVVVKLDAGARRARAAAILEYASQ
                     WPMLFGSRAAFRWRDGWRLWLHGGERLWRRASSGS"
     gene            10563..11597
                     /locus_tag="PK634_06535"
     CDS             10563..11597
                     /locus_tag="PK634_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOW97430.1"
                     /translation="MFDRLLGLFSSDIGIDLGTANTLVYVKDRGIVIREPSVVAIQAG
                     TNRVLAVGEEAKRMLGRTPGNIVAIRPLKAGVIADFEITEAMLRYFIRKVHNRRMLVR
                     PRVICAVPSGITEVEKRAVKDSATHAGAREVYLIEEPMAAAIGVGLPVQEPAGNMIVD
                     IGGGTTEVALISLAGIVLSRSVRVGGDEMDEAIVQYMKRVYNLMIGERTAEEIKIAIG
                     SAYPQGEETSMEVKGRDLVAGLPKTLTVTSEEVRNALQEPITAIVEAVRMTLERCPPE
                     LSADLVDRGVVLAGGGALLRGMDKLVAEQTGLPVHVAEDPLSAVAEGTGVVLHELAFL
                     RKVASVSRSY"
     gene            11730..12548
                     /gene="mreC"
                     /locus_tag="PK634_06540"
     CDS             11730..12548
                     /gene="mreC"
                     /locus_tag="PK634_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOW97431.1"
                     /translation="MRERGIVTWLVLAAVLLALLNLPGPVSRRTKSALRDAVAPLQEL
                     ATRSWRKLVEGARTVRGFGGLVREHQAMSAEMIHLRNRVQELRALEWENQELRRQLDY
                     GRSAERHLVPCEVIARDMTGWWHTLRLNKGADHGIGPDMAVVTQDGLIGRTIEASPKT
                     ADVLLISDPLCRVSARIAPAGAFGIVEGTGPSPAGQAICRMQFINRNQPLQAGSPVVT
                     AGLGGVFPPGLLIGYVEKVEVDEGGLYQRASVVAQADVGAATYVFVVLPGKAAP"
     gene            12545..13048
                     /locus_tag="PK634_06545"
     CDS             12545..13048
                     /locus_tag="PK634_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97432.1"
                     /translation="MKTMVLLFALIVGAVVQALAPNWRVMGQAHMPVMLGIVLYYCLS
                     PDRALMWLAAVLAGFLQDALGQIPLGYSSFCFCLVALVVSRFREDVFAGDVLMHAVSG
                     ALSAGAVTLALSVLLTRDGAVILSAGRLALKLIGSILLGAVVVPVVFHALAGADRMLG
                     NRPEEAS"
     gene            13048..14862
                     /gene="mrdA"
                     /locus_tag="PK634_06550"
     CDS             13048..14862
                     /gene="mrdA"
                     /locus_tag="PK634_06550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOW97433.1"
                     /translation="MALRDLLDEEQWRARVVLWLMIAAFGGLLYALWQMQVARGATYQ
                     AGLEKQSVRRVRLPGLRGRLFDRHGRCVADNRSGYTISLYLEELRRPGKWSNTIVRVE
                     ALLDDLSRTLGLPRQVTAEEIRNHIRYRLPLPLPVWRDVGETALARLAEQELGLSAVE
                     ISAEAVREYPFGASACHVLGYCGRADPPTEEEAFHYYIPELVGRAGLEKRYDELLRGR
                     AGGQLMLVDVSGFRFKDLGAQPAAAGRDLLLALDMRAQQVLDGALAGRVGAGVIVDPS
                     TGDVLALASSPGFDPNRFTPVLPPETWRLINEDPQRPLMNRAIASRYSPGSTFKAVVA
                     LAALASGRGSPATTFSCPGYFNLGRARFACWYTYGHGALDLRHAIERSCNVYFFQLGL
                     QAGLEPIRRAAAELGLGRATGIELEGEYPGLLPSDEWKRNTFHDGWRNGDTCNLSIGQ
                     GFLSVTPLQMALLTAALANGGTLRAPRLVLGTRAPGAEAFERLPPAPSARVDLPPDAI
                     RLVRQGMRDVVMSPNGTGRLAQIPETDAAGKTGTAEYGKKGSGLKLGWMICFAPFDEP
                     RYAIALVLEEADTGGRTTAPIMQHVLKGLFETAAPARG"
     gene            14869..15981
                     /gene="rodA"
                     /locus_tag="PK634_06555"
     CDS             14869..15981
                     /gene="rodA"
                     /locus_tag="PK634_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOW97434.1"
                     /translation="MDWTTAKWLLKRMDWFLALAVLLLLGTGIAFVYSASYRGDDLPV
                     PGFYRRQMLWAALGVAVFLAFMLTDYRRLRAAAGWFYGVSLVLLVLVLVMGKKVYGAY
                     RWLSVFGVQVQPSEFAKLALIIMLARFLGRPGRDLQHPGTLVRALILLAVPFALIVKE
                     PDLGTAMILVPVTALMLFVAGTPLRQLFVLGLVGLMLAPLGWLAMDDYQKERVHVFLN
                     PGRDPLGAGWNKIQSEIAVGSGKLWGKGWLEGKQNVLGFLPRTVAPTDFVYSVIAEEK
                     GFVGSAILLSLYAVVLGGGIRAAVEARDRAGRLMAVGMTGLLFSQVFVNIAMTIGLLP
                     IAGLPLPLISYGGSSMLTTLAGLGITQSVYTRRYSL"
     gene            15994..17610
                     /locus_tag="PK634_06560"
     CDS             15994..17610
                     /locus_tag="PK634_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOW97435.1"
                     /translation="MWSFMAKLMGKKEIHKEIIVNVESLETRVAVMEEGNLEEFYIER
                     PSDEHIVGSIFKGRIQNIEDSLQAAFVDIGMKKNAFVHYWDMIPEDAARLEAEEGIQR
                     QRTDRQKRFAPGEMAKRFPIGSEIVIQVTKAAIGTKGPRVTANLSIPGRYLVMMPGSS
                     LKGVSRKIDDGKERERLKKVLARLPQIPNIGLIVRTAGEGARKVSFVRDVRVLLETWK
                     EVEERIRTLPAPCCVYHEPDLVERVVRDSLTEDVDRIVIDSREAYDHIRELVARVSRR
                     ARGRIKLYDGAVPIFEHFEVERQLENAFRRKVWLKSGGYIVIDETEALVAIDVNTGRH
                     KGGNTQEEAILQVNTEAAREVARQMRLRNIGGLVVIDFIDMKHRRHQNAVYKTLRESL
                     RNDRARTNVLAISQLGLLEMTRQRMEESIRATTYMDCPYCEGRGKVKSTLSMSVEIQR
                     HIVEALRRHHGVPLKVSVNPVVMDRLRKEDEATLVEIEQKHGGRLTFVSDSSRHMEEF
                     TIVHAETGEELYSNVERPAPKPAAGANHCG"
     gene            complement(17659..18081)
                     /locus_tag="PK634_06565"
     CDS             complement(17659..18081)
                     /locus_tag="PK634_06565"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOW97436.1"
                     /translation="MPARILRTVLTACLPALLLSEGSARAEDPPATVTASFTAEGGEQ
                     SVTLAPGQALEVRLPGNPTTGYGWTVEAFEAQEILGGPADVEYTPQAPDRMGAGGVFT
                     FRFSAAKAGSTTLRFVYRRSWEKDEPALHTAALNVTVE"
     gene            complement(18097..18786)
                     /locus_tag="PK634_06570"
     CDS             complement(18097..18786)
                     /locus_tag="PK634_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOW97437.1"
                     /translation="MKSFVADGPPGRGRLLMLDLDGTLLDTRHDLATAVNAVRAGYGL
                     RPLSVGTVTRYVGDGLAELVQRALPGRDDDWPEAVERCRRLYRRHMLDRTAPYPGVIA
                     GLRRLKKAGYRLALVSNKPAAACRRLLRHFGLARLFDRIAGGDSTERRKPHPEPLRVT
                     MRALGFRPAESWMIGDHRTDLETARRAGVRSVFLSRGMGRRGGQRSHRTFARFSELTD
                     YFTRPHAAGKR"
     gene            complement(18839..20512)
                     /locus_tag="PK634_06575"
     CDS             complement(18839..20512)
                     /locus_tag="PK634_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOW97438.1"
                     /translation="MDSNINTISVTLEDGSQIRCAANTPVASILPQRRSKEGLEYLGA
                     LVNNDAVSLSYPLEVDSQVSLLTRADSHGFRIYRRSVCFLLGKAVKALYPEARFAVEH
                     SMSDGFYCSFELDGKTISKEHMAAIERHMRETVERDVPIERRKIAFTEAVEQFEREKQ
                     WDKYNLLRYRNPPKIVTYGCEGFSDLAHGPLAMSTGALSLFKLIPYTPGFVLQFPERE
                     TPKELSEFVPQPQLFQIFKEHKEWGRILGVNTVGRLNEIIANKEIGDFIKIAEAFHEK
                     KIARIADHIHQHRDEIKWILIAGPSSSGKTTFAKRLAVQLRVNGLRPVTVSVDNYFVS
                     RDRTPKDENGEPDFENIETLDLPLLNEHLAKLDRGEKVELPYFNFEKGDREFRGETMK
                     IEPDQMVIVEGIHGLNPRMTEAIPPAHKFKIYISALTQLNLDSNNRVSTTDNRLVRRL
                     VRDHNFRGNPALMTLGMWPSVRRGEKKWIFPFQHEANIAFNSALDYELAVLKPYVEPL
                     LAEVKPVHPQYAEARRMQDFLASFLGVSDRLVPPTSLLREFIGRSSFSY"
     gene            20743..22623
                     /locus_tag="PK634_06580"
     CDS             20743..22623
                     /locus_tag="PK634_06580"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW97439.1"
                     /translation="MNWRRFVAWGFSLFVSWHARAETRYVVPVNPGAAPPYTNWATAA
                     TTIQDAVHASAAGDTVWVTDGVYTVTAPLLVTNGIAIRSVNGRGPVVVDGQDQRRCFE
                     LGSADAQLDGLVLVRGAAVQGGGIYSLGATITNCAVYACRASNSAAGVYLAGGLLVDS
                     DVSSNRIISSSGDALWGAGICAVSSAVVRGCRVDTNSLSANNGGNVWGGGLYLENARV
                     EHSSVRANYGYSSLDDAEGGGICAFEDSRVVSCVVESNRLTASSSVAAGGGISAYDSM
                     VTGTVVRANHARAGTYGNGYGGGIMGMNADIAGCVVDSNRLYAADDQGIGGGIYLEAG
                     HLTDTAVRSNYLETVNDNAYGGGVGLFAGSLMESCDVSGNQVELEDDTVYGVGVYCVN
                     STVSVCRITGNMDISAAGGSLGGGVYVSGASGVRSCFMAGNFAVQGGGLCLVNSAAQV
                     INCTAVSNQAYYGGGLRQSAGLLLNSILVDNTTLDSNPNHYLTGGEAAYSCSAPPLPG
                     EGNDERRPQFVNAAAGNYALLPGSPGVDDGFNEDWMTNATDLAGRARIMKATVDRGAY
                     ELDPASGPLACNPVANPAEGFYPLQVVFTAAVAGADTAGLYYGISTAMGPLRSRATDW
                     PW"
     gene            22617..>22954
                     /locus_tag="PK634_06585"
     CDS             22617..>22954
                     /locus_tag="PK634_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97440.1"
                     /translation="MVTNLFDGYGSFNVSLTVSNAAGSAGTLSPGCVKVGPEIAYVAT
                     NGAHSFPFTNWVTAATNPHAAYEAAVAGTRVFVGTGTHRMASELYVDRAVAFEGQGGV
                     TDVIVHGGNTS"
BASE COUNT         3591 a         7669 c         7991 g         3703 t
ORIGIN      
        1 actacgaccg tgagaagctc caggagcgcc tggcgaagct cgcgggcggc gtggcggtca
       61 tcaacgtcgg cgcggcgacc gagacggaga tgaaggagaa gaaggcccgc gtcgaggacg
      121 cgctgcacgc gacacgcgcg gcggtcgagg aaggcatcgt tccgggcggc ggcgtggccc
      181 tgctgcgctg ccagccggca ctggccgacg tcaagctgga cggcgacgcc ggaatcggcg
      241 tggatatcgt ccgcaaggcc ctcgaggccc cgctgcgcca gctgacggcg aacgcgggcg
      301 tggacggctc catcgtcatc caggaagtca agaagcagaa gggcagctac gggtacgacg
      361 tggccaagga cgagtacgtg gacctggtca agaccgggat catcgacccg accaaggtcg
      421 cccgcagcgc gctgcagaac tcggccagca tcgcctcgct gctgctgacc accgaatgca
      481 tggtcgccga aattcccgaa aaggaaaaga agcccatgcc cggcgccggc ggcggaatgg
      541 gcggcatgga agacatgtac taagcccgga aagggcggtt gacacgaacc gggcggggtg
      601 cctatagtgc gccccgcccg gcttttcaga gactggaggc tgtaacggtg aacgtgaaat
      661 acatggaatt ggcgaaggaa aaagtgccca atatcccgct gcttatcaac atggtgtcgc
      721 gccgcgtgcg ccagttgaac cgcggcgagc ggcccctggt caagccggac aacatgcaga
      781 tgtcccccat gaacatcgcc ctcaaggaaa tcgccgaggg caagctgtct gccgaaatct
      841 cctttgtgcc cgtccgcaag acggtggaaa gcaacccgct ttccctgtag tgcccgcggc
      901 gggccggaac ggttcgcatg gccccggctc cctctccagc gcgtcccgcc gtcaagacga
      961 tggcgacgca ccggaaggcg tttcgggatt accacatcct cgagaagatc gaggccggca
     1021 ttgaactgcg cggcgccgag gtcaagtcca tccgcgaggg ccggttcagc ctcaccgaga
     1081 gccatgccct cgtggacggc ggccaggtga tgctgatggg gctgcacgtc cagcccttta
     1141 cctgttcccg ggtcgaggag cacgatcccc tgcggcccaa gcgcctgctt ttgcaccggc
     1201 gcgagatcga ccggttggcc ggccagaccg ccgtcaaggg ctgcgcgctc atcccgcttc
     1261 gcgtctactt caagaagggc ctggtgaagg tcgaactcgc gctgggcaag ggcaagcagt
     1321 ccgaggacaa gcgcgagacc ctcaagcgcc gcaccgccga gcgcgaagcc gagcgcgcca
     1381 tcgcctcgcg cacccggcgg cggggatgac cggggactgc tcgtgcctgc gcccatccgg
     1441 cattcatgga acctggctcc cgcccaagcc atcgccctcc aggggaaact ggctgcccgt
     1501 gtttctatca ggccgttgcc ctccggcatc cgctggatcg ccggactcga cgccgccttt
     1561 tcagaagacg aatgcgtggc cgccgtcgtg ctttgggata cgcggcgcaa ggaagttgtc
     1621 gagcagcgga tcgcccgcgc gccgctgcga ttcccgtata tccccggcct gctatcgttc
     1681 cgcgaggcgc cggccctgct cgccgcgctg gagaagttgg agcgccggcc ggacgtattg
     1741 atgtgcgatg ggcaggggct ggcgcacccg cgccggttcg gcatcgcgtg tcacatcggc
     1801 gttctcgcgg agattccctc cgtaggctgc gccaagagcc ttctcgtcgg cgagttccgg
     1861 gagcccgcga aacggcgcgg ttcgcgcgcg gcgctggcag accgcgggga acgcatcggc
     1921 acggtcctgt gcacgcggga cggcgtacga cccgtgatcg tcagcgtcgg gcacctggcc
     1981 aatcttgcga cggccgagaa actggtgtta cgctgcgcgg tcagctaccg cctgcccgag
     2041 cctacgcgcc tggccgacca gctttgcggc gcgaccgctc atctggtgta gagaaagggc
     2101 tcctttgcga tgtatcacag tttataaact gtgatatttt ccggcacccc aatcacgaaa
     2161 acattagtaa atatcatgat ttccaatcgt aaaatgcgtg tatcacagtt tataaactgt
     2221 gatacatgag gagggtttag tgccgggctt gggaatccga ttctggcgct agggcattgg
     2281 acagaagcac ccattgttcg ggcgatatat cctccggccg cgcgcggggc gcgatgccta
     2341 gccgatcgag aaggattccc gtcccggaat ccgtcagtcc ggcgcgagcc ttcaggaagg
     2401 gcgcgagttg cttgcgccgc ctcgcgaacg cgtcttgaag gagcttcaac agcgccgcgc
     2461 agttgcgcga ccgcggctcg cggtcgtccg gcaggcggac cagttccacg atcgcggagc
     2521 ggacctcggg cggcggaagg aagcaggtcg ggctgatctc cttgacgatg ttcacggcga
     2581 acgccgtctg tgcgaggatg ctgagcagcc cgtaatcttc cgcgcgcggc ttcgccgcga
     2641 gccgccgtgc cacctcgcgc tggacggtca ccacgatgcg cgtcggggga tgcgcggtct
     2701 ggaacaggtc cacgagaaac cggctgccga cggagtaggg caggttggcg acgaccttgg
     2761 aaatgccgcc ggcaagcagg gtttccaagt ctacatcaag gatgtcggcg tgaatcagtt
     2821 cgagatgagg agcctccgcg aaatgcgtct tcaggaaacc gaacaggacc gggtcctttt
     2881 ccaccgccac cacgcggccc gcgtagcgaa cgagccactc cgtcaatacg ccgagccccg
     2941 gcccgacttc gagcacctgt tcctcccggt gcaactcggc tgtgcgcagc aggatgcgga
     3001 ggatgttggc gtcgatcagg aagttctgcc ccagcgtccg gcgcggccgc agtccgagtc
     3061 gggacagcag ctctttcacc tcggaggggt gggtcaggtt catgggggag gtgaatggtg
     3121 aacggtgaat agtgaatagt aaggcgtgat tggaagtcca ctattcacca ttcactactc
     3181 actattcact tcttccaggg attccggcgc cgcgtgagcg cgatggcctg gcggatggcc
     3241 tcgacgatgc tggacggatt ggcccggccc cggcccgcga tatcaaaagc cgtgccgtgg
     3301 tcgggcgagg tgcggacgat gggcaggccc agcgtgacgt tcacgccggt ctcgaacgcg
     3361 agcatcttca gcgggccgag gccctggtca tggtacatgg ccaccacggc gtcgaagtcg
     3421 ccgcggacag cctggcgaaa aatgacatcc gcgggcacgg gtccgacggc attgaaccct
     3481 tctcggcgcg cggcgcggat ggcgggggcg atgacggtga tctcctcgcg gccgatggcg
     3541 ccgccgtcgc cggcgtgcgg attgaggccg caaaccccta tgcgcccgcc tcggcagccc
     3601 agccacggga gtgcttcacc ggtgatccgg atggcctcca gcacgtccgc ccttttcagc
     3661 ttccgcgcca cgtcggcgag cggcaggtgc cgggtggcga ggaccacgcg aagcgggccg
     3721 ccgaagagca tcatggcgta gcggcgcgtc ccggtcagct cggccagcag ttccgtgtgg
     3781 ccggggacat ggatgccggc tttctgaaat ccttctttgc agatcggcgc ggtgaccatc
     3841 gcgtccagcc ggccgtccag gcacgcggcc gccgcggcgc ggatccaggc gccggccgcg
     3901 cgcgaggcgt cggcgcggat ccgcccgggc ttccagtccg gagcaagcgc gggagcggga
     3961 tcccagacgg atacgcggtc gcccgggagc cgcaaccgga gggcgcgggc ctgggccctg
     4021 acgagtgcgt cagaacccac gagcacgaat cggacgttgg cgggccagcc cccgcgggcg
     4081 acggcgcgca gggcgatttc cggcccgatc ccgttgacat cgccgagggt gattccaatg
     4141 cggagcatcg cgctcatggc tcggtacggc tcgaagacgt tcagaacacc ttgacgtaga
     4201 agcgattctt caggcgcgcg atccaggcgt cgtagagacg atccatttcc tgcttgtgca
     4261 gctgttcctc tatttcctgg cgcgcgtcct ggaaggatgt gacggccgcc tgcttccggc
     4321 cctcgacctt ggccaggtaa atctcgtcgg aggcttcgat gacctcgctc aactggcccg
     4381 gctcgagatc ttttacgccg gcggccagtt cggcccgcag ctcggaggtt tgcatccacc
     4441 ccatgtcgcc gccctgcgcg gccttgggtc cctcggacgc ggtcttggcg acggtcgcga
     4501 aatcctcgcc gcccgcgagc cgctcgcgga tgcggcgagc ctcctcgatc ttggcggcgc
     4561 gctcctcggg ggtggccccg ggatgcagga cgatcagccg gagttgcgcc tcctccgggg
     4621 tccggtagcg gtcgaggttc ttctcgtagt attcccggac ctggcgcggc gcgacggcca
     4681 cgcggtcgag gatttccgtg cggcgcagga tggtaacgat gaggccgtcc ttggcctgct
     4741 cccgccattc ggcgagggtc agccgctctt cggcgagggc gtcgaggaag gccatccggt
     4801 tgttgttgaa ccgctcgctg atcaggctgt tgacctgggt gtcgatggcg cggtcgggca
     4861 gttggtggcc ctggcggttg aactcctcga cgatcagcgc gcgctcgatc aggctttcca
     4921 gcacattcgt gtaggcctcc tcgagcttct tctccaactc ggcgcccgag tgctccagct
     4981 tcagtttctg ctgaagcggg tagaccatgg cgaagacttc gctccggagg atcacgcgct
     5041 ggttcaccat ggcggcatag ccgtccacgg ccaccgaccg cgccccggcg gacgccgccc
     5101 aggccagcca ggccgccgcc atcctcccca gcatccctcg catgcgtttc atggccccag
     5161 cactatggca aaagaggcct cgccgcgcaa cagcctttcg accggtccga ttctccttgc
     5221 ctttcgggcg aggcatcccc atttctaggg ccttggaacg gacgaaaagg aaatcaccat
     5281 gagaagcgac aaggtcaaga cgggtttcga gcgcgcgccg caccgcagcc tcctgcgggc
     5341 cacggggctc accaccgagg cgatgagcaa gccgttcatc gccatctgca actcctacac
     5401 ggatatcatt cccggccacg ttcacctgcg gaaggtcggc gacctggtcc gcaaggccat
     5461 cctggcggcc ggcggcacgc cgttcgagtt caacaccatc ggcatctgcg acggcatcgc
     5521 gatgggccac gcgggcatga agtattcttt gcccagccgc gagttggtgg ccgactgcgt
     5581 cgagacgatg gtcaacgccc acctgttcga cggcatggtc tgcatcccga actgcgacaa
     5641 gatcgtcccg ggcatggtca tgggcgcgtt gcgctgcaac atcccgaccc tcttcgtcag
     5701 cggcggcccg atgcgcgcgg gcaagatgcc cgacgggcgc gccgtggacc tgatcagcgt
     5761 gttcgagggc atcggcgcgt tcaaggccgg ccacatttcg gaggagcaac tcgaagaaat
     5821 ctcgtgccat gcctgtcccg gcgccggctc ctgttccggc atgttcacgg cgaactccat
     5881 gaactgcctc tgcgaggcga tcggcctggc gctgcccggc aacggcacga tcctggccga
     5941 ggacccgaag cggcgcagcc tctggcgcgc agcgggcaag cgcatcgtcg agatggtgcg
     6001 cgaggacctg aagccgcgcg acatcgtcac gaaggagtcc ctggacaacg ccttcgtgct
     6061 ggacatggcc atgggcggca gcacgaacac cgtcctgcac acgctggcca tcgcgcgcga
     6121 ggcgggcgtg gattaccggc tcgatcgcat caacgagctg tcgaagaaaa ccccgaacat
     6181 ctgcaaggtc tcgccgtcgt cgccgtacca tgtcgaggac gtcgaccgcg cgggcggcat
     6241 cagcgcgatc ctgcgcgaga tcagccgcgt ccggggcctg ctgaacctgg attgccgcac
     6301 ggtcaccggc aagacgctcg gcaagctgat cgcgcgggcg aagagcaagg atccggcgtg
     6361 catccggccg gtcgagaacg cgtacagccc ggagggcggc ctggccatcc tctacggcaa
     6421 cctcgcgccg gagggctcgg tgatcaagac cgccggggtg gacccgaaga tgctcatgca
     6481 cgagggcccc gcgatcatct tcgagtcgca ggaggacgcc tgcgaaggca ttctcgccgg
     6541 caaggtgaag cccggccacg tggtggtgat ccgctgcgag gggccgaagg gcgggcccgg
     6601 catgcaggag atgctctcac cgacgagcta catcatgggg caggggctgg gcgacaaggt
     6661 ggcgttgatc acggacggtc ggttcagcgg cggcacgcgc ggcgcgtgca tcggccatgt
     6721 atcgcccgag gcggcggagg gcgggccgat cggcctgctg aaagacgggg acctcatctc
     6781 catcgacatc ccggcccggc ggctggacgt gaagctttcg gacggcgagt tggcggcgcg
     6841 caggcagtcg tggcgcgccc cggagccccg attcaagacc ggctggctgg cgcgctacat
     6901 caagtttgcc acgagcgcca gcacgggcgg cgcgctgaag gagtaggccg gcggatcaag
     6961 ggtttttgtc ggcggccacg gcgagggccc cctgttggag gagggacttg aggtaggccg
     7021 tcacggacac ccgggcctcg tggaacgtca gcctgttctt ctccgcgaag ctgtcgatga
     7081 ccgcctcgac ggtgcgctgc ccgtcgcaca gcgtccatac ctcgaccccc aaagcatcca
     7141 gggtcagcgt gcggaacgcg ggcgggcgca cgacccagga gaggggcggg accagccagc
     7201 ccggccggcg cgtgggcacc tcgatgcgta gctcgccgtg ctgtgccgcg ggctgcgacc
     7261 ggacggccgc attgtggatc atccgggccc ggagcatggc gctccagccg cccgccggcg
     7321 aattcgtggc ctgcgcgggc gccttcaagg cggccacctg acgcccgggc agcaggcgat
     7381 atacccgtcc ggcggttcgg cgttcgtgtc gtcaccgcgc gggcgtatcc gctccgcgtg
     7441 gtatagccgt ccgtcgcggg ggcagatcca ggcggctgct tcgtagcgtc cgcgccgttg
     7501 gagcaggccg cgcgggatga aatggccctc gaccttttcc atgcgatggc cggctttgtc
     7561 cgaagaggag cgttgatgct cctgcacgcg gccggctgtt tgcgcgtcga gccactcgcc
     7621 gacggatgtt ttcatccagt gcggcacgag cccgagccgg cggaacgacc atcggtccgg
     7681 ggcgcgcgat ccggcgaatt ccaagcccgc aagcgccggc tgcgcgacgc attgggccag
     7741 tttcatggaa gccggcgcca cgcaatccaa tccgaaagcg cgccaccgtc ggccgccggc
     7801 cttcgtctcg ggctccgcgg cgaagccgtc caggatgctc cgctccagcg gatcctcggg
     7861 ttcgcccggc cagaggaaga ccaattccac caggcacccg ttctccgcga agtaggcgcc
     7921 gaaccgcgag gacgaaccgt ggggccgacg gacctggagg ccgtgccagg cgccggcgga
     7981 acgggcgctg acggccgccc cgctcgagga ttccagccgg gcgcggtagt cgcccatcat
     8041 gcgctcgaag tccggcgcgc cggggacctt cctccagtcg aattcgaggc ggaaccggtc
     8101 ccggtcggcg aacgcgcagc ggcccttctc catgtcgcgc gagaactgga gcatttccca
     8161 gtccgcgggc aattcgagcc ggaaatggcg ccagatccag gtctggccgg ctgggccgtg
     8221 gtccgcgttc atcgggtgca gcatggccgc gccatcggtg tttggcaaaa aaattcgcag
     8281 gcgggccggt cagggccgcg tgacgcgcac gcggtacatc acggcgccga cgggcggggt
     8341 gtccgtgtgc gcctgtccgc cgtcgatccc ggcggacacg tggttggtga gccagcgccc
     8401 ggccagcagg ttggtcgtgc tggtcaggta gtaggtgttg cccgaccggg cggaccacga
     8461 gatggcgcag gtggccccgg tgaccgcatg gatgcccgcc gcgaagcagc tggaggtgtc
     8521 ggccggatgg gtccccgtga tgaactcgcc gaggtcgctc atcgagtccc cgtcgccgtc
     8581 gttggtcccg tcgctggcga tcaggtccca caggttgctc gcctcccagc cgtccgccat
     8641 caggtccgcg tcggaatcca gggacccgtc gaaagggaac aggttgggct cgctgccgta
     8701 cgcggtctgc caggcctcgt aggtctgctt caggccggcc cagacgaggt cgctgttggt
     8761 catgcgcagg aggaggttgg tctcgaattc cacgatgaaa ggcggcagca attccgactg
     8821 gccggcgaag atggcgcacg tgtagatccg gttggtctgg aggaggccgc gcccgcgcag
     8881 ttccaccagc cggtcgaggt tgagccagcg ccactgcttc ccgccgaagt tgccgatgtg
     8941 cgggaccggc gccgcgtcgt cgtgccgcgt gaagtagttc gtgtccttcg gccgccagat
     9001 gccggacatc cagaggttgg tctcgtcgcg gtgcgaggcg gtgccgccgc cccagagcac
     9061 ggccggcatc cacgtctcgg ccgtgtactg caggcccgtg accgtgccgc tcaagtgggc
     9121 gagcacggag ctggtcggcg gccaggcgat gaatccgccg gcgatgtcgg agggcggcac
     9181 gccgcactgc cccatcagga aggcgacgtc ggcgtagctg tgctggccct cgtccgattc
     9241 gtgcgcgtgc ggatcgacct cgaaacccat attcttcagc caggccacga cgttggcgcc
     9301 gccggtttcc ggcgtgccgt ggtcgaagtt ggtcaccgcg cgcaggaacg accagtcgga
     9361 ctgccagttg aacatgacgc cgtgcgagga aagcatgctg gcgaagacca ccaggttcga
     9421 gcgctcgcgg aggaaagcgg gcggattggt gtggtaatcc ccgccctcct cgttgtgcat
     9481 gacgatgtgg acgtagatcg gaggatccac ggcaggcgcg cgcgcggcga gcgccaggcc
     9541 cagcagtgcg atgaacgccc ccctgctcac ggcggatagg ataccgccgg cgggcggcgg
     9601 ctgttaagcg gcccggcggc tcgcggattg ctttgaggcg gcggcttgat aaactccggc
     9661 catggcgcaa gggccctatg actgcctgta tctttccccg cacctcgacg acgccatcct
     9721 ctcctgcgcc gggcagatca gcctgcggac ccgcgaaggc cgctcggtcc tgatctacac
     9781 cgcgatggcg gcggaacaag tgccctcgcc gttacaggcc aacggcgcgc ggttcctcaa
     9841 gctgtgcgga ctggggcccg atgccggccg cgtccggcgc gccgaggacg cccgggcctg
     9901 cgcgcgcgtc ggggcgaagc cggatcacgg cgacctgccg gaggcggtct accggtgcga
     9961 cgcgtcgggg cgggagttgt acccgacgca cgagtcgctg tttgaagcga tccgtcccga
    10021 ggacgagggc ctcatggacc ggttggcgcg gagcttcggc gccctgccgc gcgcggccgc
    10081 ggtgttcgct ccgctcggcg cgggcggtca cgtggatcat gtcctcgtcc gccgcgcggc
    10141 ggagcaggtc ttcggcgcgg ggctttcgta ctacgaggac tacccctatg ccgtttgccg
    10201 ggacgtggtg cgccggctga cgcagccgcg ccgggcctgg tcgacggtgg tcgtcaaact
    10261 ggacgccggg gcgcggcggg cgcgggcggc ggcgatcctc gaatacgcgt cgcagtggcc
    10321 gatgctgttc ggcagccggg cggcctttcg atggagggac ggctggcgcc tctggcttca
    10381 cggcggcgaa cggctctggc ggcgcgcgtc atcgggatcg taacaattat cttcacaccc
    10441 gtgaagcgat ttgttgcgaa tgggcgggcg cgggcggcgt cccggtttta tcgcttgacg
    10501 ttttgcgcgg tgttgtcgca aagtacgcgg cgaaacgagg cagcagggga caagggccat
    10561 tcatgtttga cagactttta gggctttttt ccagcgatat cggcatcgac ctgggcacgg
    10621 ccaacacgct ggtctacgtc aaggaccgcg ggatcgtgat ccgggagccg tccgtcgtgg
    10681 ccatccaggc cggcaccaac cgcgtgctgg ccgtgggcga ggaggccaag cggatgctgg
    10741 gccgcacgcc gggcaatatt gtcgccatcc gtcccctgaa ggccggcgtg atcgcggact
    10801 tcgagatcac cgaggccatg ctccgttact tcattcgcaa ggtgcacaat cgccgcatgt
    10861 tggtccggcc gcgcgtcatt tgcgcggtcc ccagcggcat cacggaagtt gaaaaacgcg
    10921 ccgtcaagga ttcggccacc cacgccggcg cgcgggaggt atatctgatc gaggagccga
    10981 tggcggccgc catcggcgtc gggctgccgg tccaggagcc ggcgggtaac atgattgtgg
    11041 acatcggcgg cggcaccacc gaagtggcgc tcatttcgct cgccggcatc gtcctctccc
    11101 ggagcgtccg ggtgggcggc gatgaaatgg acgaggcgat cgtccagtac atgaagcggg
    11161 tttataattt aatgataggg gagcgcaccg ccgaggaaat caagatcgcc atcggctccg
    11221 cctaccccca gggagaagaa acgagcatgg aggtcaaggg ccgcgacctg gtggccggtc
    11281 tgcccaagac cctgaccgtg acgtccgagg aagttcgaaa cgctttgcag gaaccgatca
    11341 ccgccatcgt cgaggccgtg cgcatgaccc tggaacgctg tccgccggaa ctgtcggcgg
    11401 acctggtgga ccggggcgtc gtcctggccg gcggcggcgc gctgttgcgg ggcatggaca
    11461 agctggtggc cgagcaaacc ggcctgccgg tccatgtggc cgaggatccc ttgagcgccg
    11521 tcgccgaggg gaccggcgtc gtcctgcatg agctggcctt cctccgcaag gtggccagcg
    11581 tcagccgcag ctactgatcc tctccggatg tccgatcgct cccgtccgcc gggcggatgg
    11641 cttctccccg accagaacgg aatgtgcgtg gcgtattgga ccataggttc gggagccggg
    11701 acccccggcg gacgtgaccg ggagagggca tgagggaacg gggtatcgta acctggctgg
    11761 tgctggcggc ggtgctgctg gccctgctca acctccccgg gccggtctcc cgccggacca
    11821 agtcggcgct gcgcgacgcc gtcgcccccc tccaggaact ggcgacccgg tcctggcgta
    11881 aactcgtcga gggcgcccgc acggtccggg gcttcggcgg cctggtccgc gagcaccagg
    11941 cgatgtcggc cgagatgatc cacctccgca accgcgtcca ggagctccgc gcgctggaat
    12001 gggagaacca ggaactgcgc cgccagctcg actacggccg ttccgcggag cgccacctgg
    12061 tcccgtgcga ggtcatcgcg cgcgacatga ccggctggtg gcacacgctg cgcctgaaca
    12121 agggggcgga ccacggcatc ggcccggaca tggccgtggt cacgcaggac ggcctcatcg
    12181 gccggaccat cgaggcgtcc ccgaagaccg cggacgtgct gctgatctcc gatcccctct
    12241 gccgcgtctc cgcgcgcatc gcgccggccg gcgcgttcgg catcgtcgag ggcaccggcc
    12301 cgtcgcccgc ggggcaggcg atctgccgga tgcagttcat caaccgcaac cagcccctgc
    12361 aggcgggcag cccggtggtc acggccggcc tgggcggcgt gttcccgccc ggcctgctga
    12421 tcggctacgt cgagaaggtc gaggtggacg agggcgggct gtaccagcgc gccagcgtgg
    12481 tggcccaggc ggacgtcggg gcggcgacgt acgtgttcgt ggtgctgccc ggaaaggcgg
    12541 cgccatgaag accatggtcc tcctcttcgc gctcatcgtg ggcgcggtgg tccaggcgct
    12601 ggcgcccaac tggcgggtga tgggccaggc ccacatgcca gtcatgctgg gcatcgtcct
    12661 ctactactgc ctttcccccg accgcgcgct gatgtggctg gccgccgtgc tggcgggatt
    12721 cctgcaggac gcgctcgggc agatccctct cggctattcc tcgttctgct tctgcctcgt
    12781 cgccctcgtc gtcagccggt ttcgcgagga cgtgttcgcg ggcgatgtcc tcatgcacgc
    12841 cgtctccggc gcgctctcgg cgggcgcggt gacgctggcg ttgagcgtcc tgctgacccg
    12901 cgacggcgcg gtcatcctgt ccgccggccg cctcgcgctg aagctgatcg gctccatcct
    12961 gctcggcgcc gtcgtcgtcc ccgtggtctt ccacgccctc gcgggcgcgg accggatgct
    13021 gggcaaccgc cccgaggagg cctcctgatg gccctccgcg acctgctcga cgaggaacag
    13081 tggcgcgccc gggtcgtgct ctggctcatg atcgccgcgt tcggcggcct gctctacgcg
    13141 ctctggcaga tgcaggtggc ccgcggcgcc acctaccagg ccggcctgga gaagcagagc
    13201 gtgcggcgcg tccgcctgcc cggcctgcgc ggccggctgt tcgaccgcca cggccggtgc
    13261 gtggccgaca accgctccgg ctacacgatc tcgctctacc tcgaggagct gcgccggccg
    13321 ggcaagtgga gcaacaccat cgtccgcgtg gaggccctcc tcgacgacct gtcccggacg
    13381 ctcggcctgc cgcggcaggt gacggcggag gagatccgca accacatccg ctaccgcctg
    13441 ccgctgccgc tgcccgtgtg gcgcgacgtc ggcgagaccg cgctggcccg cctcgccgag
    13501 caggaactcg ggctgtccgc cgtcgagatc agcgccgagg ccgttcgcga atacccgttc
    13561 ggcgcgtcgg cgtgccacgt cctgggctac tgcggccgcg cggacccgcc gacggaggag
    13621 gaggccttcc actactacat ccccgagctg gtcggccgcg cggggctgga gaagcgctac
    13681 gacgagctcc tgcgcggccg cgccggcggc cagctgatgc tggtggatgt ctccgggttc
    13741 cgcttcaagg acctcggcgc gcagccggcc gcggccgggc gcgacctgct gctcgcgctc
    13801 gacatgcggg cccagcaggt cctggacggc gcgctcgccg ggcgcgtcgg cgccggcgtc
    13861 atcgtggacc cgtccacggg cgacgtcctg gccctggcca gctcgccggg gttcgatccc
    13921 aaccgtttca cgcccgtgct cccgccggag acgtggcggc tcatcaacga ggatccccag
    13981 cgccccctga tgaaccgcgc gatcgcctcc cggtactccc ccggcagcac gttcaaggcg
    14041 gtcgtcgccc tcgccgcgct ggcctcgggc agggggtcgc ccgccacgac gttctcgtgt
    14101 cccggctact tcaacctggg ccgggcgcgc ttcgcctgct ggtacacgta cggccacggc
    14161 gcgctggacc tgcgccacgc gatcgagcgc tcctgcaacg tctacttttt ccagctcggc
    14221 ctgcaggccg ggctggagcc gatccgccgg gccgccgccg agctcggcct gggccgcgcg
    14281 accggcatcg agctcgaggg cgagtacccc ggcctgctgc cgagcgacga gtggaagcgg
    14341 aacaccttcc acgacggctg gcgcaacggc gacacgtgca acctttccat cgggcagggc
    14401 ttcctgtccg tgacgccgct gcagatggcg ctgctcacgg ccgcgctggc caacggaggg
    14461 acgctgcgcg cgccccgcct ggtgctgggc acccgcgcgc ccggggcgga ggcgttcgag
    14521 cggctgcctc ccgcgccgtc cgcccgcgtc gacctgccgc cggacgcgat ccggctcgtg
    14581 cgccagggca tgcgcgacgt ggtgatgtcg cccaacggca ccggccggct cgcgcagatc
    14641 ccggagaccg acgccgccgg caagaccggc accgccgagt acgggaagaa ggggagcggc
    14701 ctgaagctcg gctggatgat ctgcttcgcg cccttcgacg agccgcgcta cgccatcgcc
    14761 ctcgtgctcg aggaggccga caccggcggg cggacgaccg ccccgatcat gcagcacgtg
    14821 ctcaagggct tgttcgaaac cgccgcgccg gcgcggggat gacgcgacat ggattggacc
    14881 accgccaaat ggctgctgaa gaggatggac tggttcctcg cgctcgccgt cctgctgctc
    14941 ctcggaaccg gcatcgcctt cgtctacagc gcctcctacc gcggcgacga cctgcccgtg
    15001 ccgggcttct accgccgcca gatgctctgg gccgcgctcg gcgtcgcggt cttcctggcc
    15061 ttcatgctca ccgactaccg ccgcctgcgc gccgccgcgg gctggttcta cggcgtgagc
    15121 ctcgtgctcc tggtcctcgt gctggtcatg ggcaagaagg tgtacggggc ctaccgctgg
    15181 ctctccgtgt tcggcgtcca ggtccagccc tccgagttcg cgaagctggc gctgatcatc
    15241 atgctggcgc gcttcctcgg ccggcccggc cgcgacctgc agcatcccgg cacgctcgtg
    15301 cgcgcgctga tcctgctcgc cgtccccttc gcgctgatcg tcaaggagcc ggacctgggc
    15361 acggcgatga tcctcgtgcc cgtgacggcg ctgatgcttt tcgtggcggg cacgcccctt
    15421 cggcagctgt tcgtgctcgg gctggtcggc ctgatgctgg ccccgctggg ctggctggcg
    15481 atggacgact accagaagga gcgcgtccac gtcttcctga acccgggccg cgacccgctg
    15541 ggcgccggct ggaacaagat ccagtccgag atcgccgtgg gctcgggcaa gctgtggggc
    15601 aagggctggc tcgagggcaa gcagaacgtg ctgggcttcc tgccccgcac cgttgcgccc
    15661 accgatttcg tgtattccgt gatcgccgag gagaaggggt tcgtcgggtc ggcgatcctg
    15721 ctgtcgttgt acgccgtcgt actgggagga ggcatccggg ccgccgtaga ggcccgggac
    15781 cgggcgggcc ggctcatggc cgtgggcatg accggcctgc tgttcagcca ggtgttcgtg
    15841 aacatcgcga tgacgatcgg gctgctgcct atcgccggcc tgccgctgcc gctgatcagc
    15901 tacgggggct cctccatgct gaccacgctc gcgggcctgg gcatcaccca gagcgtctac
    15961 acgcgccggt attcactgta aggagaagaa aacatgtggt ccttcatggc caagctgatg
    16021 ggtaagaaag agatacacaa agagatcatc gtcaacgtcg agagcctcga gacgcgggtc
    16081 gccgtcatgg aagagggcaa cctggaggag ttctacatcg agcgcccgag cgacgagcat
    16141 atcgtgggca gcatcttcaa gggccggatc cagaacatcg aggacagcct gcaggccgcc
    16201 ttcgtggaca tcggcatgaa gaagaacgcc ttcgtccact actgggacat gatcccggag
    16261 gacgccgcgc gcctggaggc cgaggagggc atccagcgcc agcggaccga ccgccagaaa
    16321 cggttcgcgc ccggcgagat ggccaagcgc ttcccgatcg gctccgagat cgtcatccag
    16381 gtcaccaagg ccgcgatcgg caccaagggc ccgcgcgtga cggcgaactt gagcatcccc
    16441 ggccgctacc tggtgatgat gccgggcagc agcctgaagg gcgtgtcgcg caagatcgac
    16501 gacggcaagg agcgcgagcg gctcaagaag gtgctcgccc gcctgccgca gatcccgaac
    16561 atcggcctca tcgtccgcac cgcgggcgag ggggcgcgca aggtgagctt cgtgcgcgac
    16621 gtgcgcgtgc tgctggagac ctggaaggaa gtcgaggagc gcatccgcac cctcccggcg
    16681 ccgtgctgcg tctaccacga gcccgacctc gtcgagcgcg tcgtccgcga ctcgctgacc
    16741 gaggacgtgg accgcatcgt catcgactcc cgcgaggcct acgaccacat ccgcgagctg
    16801 gtcgcccgcg tctcgcgccg cgcccgcggc cgcatcaagc tctacgacgg cgccgtgccg
    16861 atcttcgagc acttcgaggt cgagcgccag ctcgagaacg ccttccgccg caaggtctgg
    16921 ctcaagtccg gcggctacat cgtcatcgac gagaccgagg cgctcgtggc catcgacgtc
    16981 aacaccggcc ggcacaaggg cggcaacacg caggaggagg ccatcctcca ggtcaacacg
    17041 gaggccgccc gcgaggtcgc ccgccagatg cgcctgcgca acatcggcgg gctcgtggtc
    17101 atcgacttca tcgacatgaa gcaccgccga caccagaacg ccgtctacaa gacgctccgg
    17161 gagtcgctgc gcaacgaccg cgcccgcacg aacgtgctgg cgatctccca gctcggcctg
    17221 ctcgagatga cccgccagcg catggaggag agcatccgcg cgaccaccta catggactgc
    17281 ccgtactgcg aggggcgcgg caaggtgaag tccaccttga gcatgagcgt cgagatccag
    17341 cgccacatcg tcgaggccct gcggcggcac cacggcgtgc cgctgaaggt cagcgtcaac
    17401 cccgtcgtca tggaccggct gcgcaaggag gacgaggcca cgctggtcga gatcgagcag
    17461 aagcacggcg ggcggctgac gttcgtgtcc gactcctcgc ggcacatgga ggagttcacc
    17521 atcgtccacg ccgagaccgg cgaggagctc tactccaacg tcgagcggcc cgccccgaag
    17581 ccggcggccg gggcgaacca ctgcgggtag cgcggaccga acgggtgacg gggagagccg
    17641 gcttcgccgc ggcgggccct actcgaccgt gacgttcagc gccgcggtat ggagcgccgg
    17701 ctcgtccttt tcccaggagc ggcggtagac gaagcgcaag gtcgtcgagc cggccttcgc
    17761 cgcggagaag cggaacgtga acacgccgcc cgcgcccatg cgatccggcg cctggggcgt
    17821 gtactccacg tccgccggcc cgcccaggat ctcctgcgcc tcgaaggcct ccaccgtcca
    17881 cccgtacccc gtggtcgggt tgccgggcag gcggacctcc agggcctggc ccggggccag
    17941 cgtcacggat tgttcgccgc cttccgcggt gaagctcgcg gtgacggtgg ccggcgggtc
    18001 ttccgcgcgg gcgcttccct ccgacaggag gagggccggc aggcaggcgg tcaggacggt
    18061 ccggaggatt cgggcaggca tggcattttc cctcgatcag cgtttcccgg cagcatgggg
    18121 ccgcgtgaaa tagtccgtca actcggaaaa gcgcgcgaag gtccggtggg accgctggcc
    18181 gccgcgccgg cccatgcccc gggacaggaa cacgctccgc acgcccgcgc gcctcgccgt
    18241 ttccaggtcc gtccggtggt cgccgatcat ccacgactcg gccggtcgga accccagcgc
    18301 gcgcatcgtg acccgcaggg gctccgggtg cggcttcctc cgctcggtcg aatcgccgcc
    18361 cgcaatgcgg tcgaacagcc gcgcgaggcc gaagtggcgc aggaggcgcc ggcaggcggc
    18421 ggcgggcttg ttgctgacga gggccagccg gtagcccgcc tttttcagcc ggcggagccc
    18481 ggcgatcacg ccggggtagg gcgcggtccg gtcgagcatg tgccgccggt agaggcggcg
    18541 gcagcgctcg acggcctcgg gccagtcgtc gtcgcgcccc ggcagcgcgc gctgcacgag
    18601 ttccgccagg ccgtcgccga catagcgcgt gaccgtgccg acggacagcg ggcggagccc
    18661 gtagccggcg cgcacggcgt tgaccgccgt ggccaggtcg tggcgggtgt cgagaagcgt
    18721 cccgtccagg tcgagcatca gcagccggcc gcggcccggc ggaccatccg caacaaaact
    18781 cttcacgggt gtgaatgctt ttgttgcgct cgcgccgcgg cccgcgggcc cgcccgtcct
    18841 agtagctgaa gctgctgcgg ccaatgaatt ccctcaacag cgaggtcggc ggcacgagcc
    18901 ggtccgacac gcccaggaaa ctcgccagga agtcctgcat ccgccgcgcc tccgcgtact
    18961 gcgggtggac gggcttgacc tcggccagca ggggctcgac gtagggcttg agcaccgcga
    19021 gctcgtaatc cagcgccgag ttgaacgcga tgttcgcctc gtgctggaac gggaagatcc
    19081 acttcttctc gccgcgccgc acgctgggcc acatgccgag cgtcatcagc gccgggttcc
    19141 cccggaagtt gtggtcgcgc accagccggc gcacgagccg gttgtccgtc gtggacaccc
    19201 ggttgttcga gtcgaggttc agctgcgtca gcgcgctgat gtagatcttg aacttgtgcg
    19261 ccggcgggat ggcctcggtc atccgcgggt tcaggccgtg gatgccctcg acgatgacca
    19321 tctggtccgg ctcgatcttc atggtctcgc cccggaactc gcggtcgccc ttctcgaagt
    19381 tgaagtaggg cagctccacc ttctccccgc ggtccagctt ggccaggtgc tcgttcagca
    19441 gcggcaggtc cagggtctcg atgttctcga agtccggctc gccgttctcg tccttcggcg
    19501 tccggtcccg gctgacgaag tagttgtcca cggagaccgt caccggccgc agcccgttga
    19561 cgcggagctg gacggccagc cgcttggcga acgtggtctt gcccgaggag gacggcccgg
    19621 cgatgaggat ccacttgatc tcgtcccggt gctggtggat gtggtcggcg atccgggcga
    19681 tcttcttctc gtggaacgcc tcggcgatct tgatgaagtc gccgatttcc ttgttggcga
    19741 tgatctcgtt gaggcggccg accgtgttga cgccgaggat ccggccccac tccttgtgct
    19801 ccttgaagat ctggaacagc tgcggctgcg gcacgaactc ggacagctcc ttcggcgtct
    19861 cgcgctccgg gaactgcagg acgaacccgg gcgtgtaggg gatcagcttg aacagcgaca
    19921 gggcgcccgt gctcatggcc agggggccgt gggccaggtc cgagaagccc tcgcacccgt
    19981 aggtcacgat cttgggcggg ttgcggtagc gaaggaggtt gtacttgtcc cactgcttct
    20041 cgcgctcgaa ctgctcgacg gcctccgtga aggcgatctt gcgccgctcg atgggcacgt
    20101 cgcgctcgac cgtctcgcgc atgtggcgct cgatggcggc catgtgctcc ttggagatcg
    20161 tcttaccgtc cagctcgaag ctgcagtaga acccgtcgct catcgagtgc tcgaccgcga
    20221 agcgcgcctc ggggtacagg gccttgaccg ccttgcccag caggaagcac accgaccggc
    20281 ggtagatgcg gaacccgtgc gagtccgccc gcgtgaggag cgagacctgg ctgtccacct
    20341 ccagcgggta ggacagcgag acggcgtcgt tgttgaccag ggcgccgagg tattccaggc
    20401 cttccttcga gcggcgctgg ggcaggatgg acgccaccgg ggtgttggcg gcgcagcgga
    20461 tctgggaacc gtcctccagc gtgacgctga tggtgttgat gttcgaatcc atgacttctc
    20521 tccttcgtgg cttccgaaaa aaggattcca gggcggaaaa aactcctttt cccagccctg
    20581 gaaaaacacg tcctgttttt ccaatatttt cgtctaatac taccccgctt tttcaagcat
    20641 gggaaacatt ttctgcctcg gtggccggcg cccgcccgcg gcattgcggt cttgtcatgg
    20701 cgggtgcggc ggttaagatg acttcagaat cagggggggg ccatgaactg gaggcgtttt
    20761 gtggcgtggg ggttcagtct gttcgtctcg tggcatgccc gcgcggagac gcggtatgtc
    20821 gtgccggtga atcccggcgc ggcgccgccg tacaccaact gggcgacggc ggcgacgact
    20881 atccaggacg cggtccacgc ctcggcggcg ggcgatacgg tatgggtgac ggatggcgtg
    20941 tacacggtca ccgcgccgct cctcgtgacc aacggcatcg cgatccgcag cgtcaacggg
    21001 cggggcccgg tcgtcgtgga tggccaggat cagcgccgct gtttcgagct gggatcggcg
    21061 gacgcgcaac tcgacgggtt ggtgctggtc cgcggggccg cggtgcaggg cggcgggatt
    21121 tattcgctcg gcgcgacgat caccaactgc gccgtctatg cctgccgcgc atccaactcg
    21181 gcggccgggg tctacctggc cggcgggctg ctggtggata gcgacgtgag cagcaaccgg
    21241 atcatctcgt cttccggcga cgcgctttgg ggcgccggca tttgcgccgt cagctccgcg
    21301 gtcgtccgcg gatgccgcgt ggacaccaat tcgctttcag ccaacaacgg aggaaacgtt
    21361 tggggcggcg gactgtacct ggagaacgcg cgggtggaac attcgagcgt gcgggcgaat
    21421 tacgggtact ccagcctgga tgacgccgag ggcggcggca tctgcgcctt cgaggacagc
    21481 cgggtggttt cgtgcgtcgt ggaatccaac cggttgacgg cttcctcctc ggtggccgcc
    21541 ggaggcggca tctcggccta cgactccatg gtcaccggca ccgtcgtgcg ggcgaaccac
    21601 gcccgcgcgg gcacctacgg caacggatac ggcggcggca tcatgggcat gaacgcggac
    21661 atcgccggct gcgtggtcga ttcgaaccgc ttgtacgccg cggacgacca gggcatcggc
    21721 ggcgggatat atctggaagc aggtcatctg acagacaccg cggtccggag caattacctg
    21781 gagaccgtga acgacaacgc ctacggcggc ggcgtcggcc tgttcgccgg cagcctgatg
    21841 gagtcctgcg acgtgtccgg caaccaggtc gaactggagg acgacaccgt ctacggggtc
    21901 ggggtgtact gcgtcaattc aacggtttcg gtgtgccgca tcacggggaa catggatata
    21961 tcggcggccg ggggcagcct cggcggcggc gtgtatgtct ccggcgcatc gggggtccgg
    22021 agctgcttca tggccggtaa cttcgccgtg caaggcgggg gcctttgcct cgtgaattcg
    22081 gcggcccagg tgatcaattg cacggcggtc agcaaccagg cttattacgg cggcgggctg
    22141 cgccagtcgg ccggcttgct gctcaacagc atccttgttg acaacacgac gctggattcc
    22201 aatcccaacc actatctgac tggaggggag gccgcgtatt cctgttccgc cccgccgctg
    22261 ccgggcgagg gcaacgacga gcgccggccg cagttcgtca acgccgccgc cggcaactat
    22321 gccctcctgc ccggttcgcc gggcgtggac gacggattca acgaggactg gatgaccaac
    22381 gccacggatc tcgccggccg tgcgcgcatc atgaaggcaa ccgtggaccg cggggcctat
    22441 gagctcgatc ccgcgagcgg cccgctcgcg tgcaacccgg tggcgaatcc ggcggagggc
    22501 ttctatccgt tgcaggtggt gttcaccgcc gcggtggcgg gcgcggacac ggcgggcttg
    22561 tattacggga tttcaacggc gatgggtcct ttgagatcga gggctacgga ttggccgtgg
    22621 tgacgaacct cttcgacgga tacggaagct tcaacgtgtc gctgacggtc agcaacgcgg
    22681 ccgggtcggc cggcacgctg agccccgggt gcgtgaaggt cgggccggag atcgcctacg
    22741 tggccaccaa cggcgcgcac agcttcccgt tcaccaactg ggtgacggcc gcgaccaacc
    22801 cgcacgcggc gtacgaagcg gcggtggccg gcacgcgcgt cttcgtcggg acgggcaccc
    22861 atcgcatggc cagcgagctc tacgtggatc gcgcggtcgc cttcgaaggg cagggcggcg
    22921 tgacggacgt gatcgtgcat ggcggcaaca cctc
//
LOCUS       DAOOTK010000041        22923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1552_length_22923_cov_6.823815, whole genome shotgun sequence.
ACCESSION   DAOOTK010000041 DAOOTK010000000
VERSION     DAOOTK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22923
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_22923_cov_6.823815"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..656
                     /locus_tag="PK634_06590"
     CDS             90..656
                     /locus_tag="PK634_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOW97441.1"
                     /translation="MDGITLLIVAAVVVGLFLLVTFNTLVGRRNKVRNAFGTVDVLLK
                     KRYDLIPNLVATVQGYMQHERTVLQQITDLRSRAMGGPLPPEQLFRVNAQLGQLLGGV
                     RAAIENYPQLKASQNALQLQAALNEVEEQISAGRRAYNAAVTDLNNALEMFPSNLVGS
                     LFGFQKAVFFEAGEAERGAVPVQLGGAP"
     gene            653..1720
                     /locus_tag="PK634_06595"
     CDS             653..1720
                     /locus_tag="PK634_06595"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HOW97442.1"
                     /translation="MTSEPPSAADSAAPGSPVRTEEEFRAFYQGVLLPELESVDRERR
                     AVRGRVLVLWLSLLGLIFLSLLIFGGLQASGPPGAAVVVVVGGIGLALFGFWLAAFAR
                     LSRAKRAYIARYKNTVIGHIIRFIEPGLAYEPDSFVAEAVYQKSGLFPRAWDRYRGDD
                     FIHGRAGRTPLFFSELHTEYKTRQRTSDNRTRETWHTIFHGLFFVCEFNKTFQGRTVV
                     LPDRLEGLLGDLGAALQFSRSQYGELVKLEDPEFERMFAVYGSDQVTARYILSTSLMA
                     RLMAFRGKARQDIRLAFVDSNLYAAVSFNRDLFEPRVWRSLIDYEEIRGYFEILQLVI
                     GIVEDLSLNTRIWGERALSGA"
     gene            complement(1730..2569)
                     /locus_tag="PK634_06600"
     CDS             complement(1730..2569)
                     /locus_tag="PK634_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOW97443.1"
                     /translation="MTTAISRRRFLKMTGPLGAIGLVSSYPFFIERYLVATNTYRIPV
                     PRLPPAFAGLRVVHLSDLHFGALMPMRVIADVIRRANELGGDLIVCTGDYVHERNAAR
                     QIDAVWPELARLSAPLGVHSVLGNHDHWADTARSEHWLEQTGQGLRHRVAALEKDGQQ
                     LWLAGAGDLWEDHRRLDDLLARIPDAGCRIVLAHNPDTADTPFAGRADLMISGHTHGG
                     QVRLPFLGAPILPVRNKRYSSGLCRSPRGLGVFISRGIGWAYLPVRFNCLPEIAVLEL
                     RPA"
     gene            complement(2574..5360)
                     /locus_tag="PK634_06605"
     CDS             complement(2574..5360)
                     /locus_tag="PK634_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOW97444.1"
                     /translation="MNTRFILLLATSLMLGHDGPAAEPAAPSPAPHKMTGRVLDGIGA
                     ALAGIAVHCYLPYVNSSGWEAATDADGRFEFTRLPPWEQVVAAVDPSGRLAPALVEPA
                     IAGRSHDIVMRPDWGATAGRVVDEAGEPVAGATVTVYLASGKLLQDADSRRRYGGGEH
                     MVRWSRSSVTDAKGEFSVEAFTATPRLVEVIKDGYLDMAFPVATSPCVLTVYAREDIR
                     RELKTLGGPMGAEYLRCLHALDPPWLGAETLDALLPRVQAIADGRESHALVTPELARQ
                     ALFLLSPPRPARGTVSDAEGRPLAGIPVYYGYPYVGGDHRMTTDAEGRFETEPLRYAG
                     GFLAVVDPSGARAPQMKEGIEGGRAYDFTLTTNRARLAGVATDAQGRPLPQCRITAVL
                     LPARILDEEFGAPPRFYLSSLEPLIGESPWAVTTTSGPEGRFELGVPPASRAQPAFAE
                     KEGYLGAMALASNTPVVLALMPLEELGDALGARPPAEQVALLRRLTPPAMKSLPVDVK
                     KSIRETLQAISNDAADTTPSAAAEAEGLLRLLNLEACPLEANDYAGAFSAFAKEMDAH
                     YPFFDLKGIRGNWEAAKVNLAAEAAACTNDAEFMRVMLAAILVLRDGHMHIADPRVEP
                     AFPETRYGGVSFHPATEDRVVVMESFPELAATLPPGTVVTKVNGSPARAWFEEQAESL
                     WEKGGYPSRQRARLFAYRYGFRWEEGDFEKPPEFEIEYVPAGTTEPATLKRTADRETR
                     GWPHAYALPPGLREGPGVFHAVLSNGYGYAWCRRMQDEAAFIDIVKGFQGAPGLIVDL
                     RGCGGGGGGRASAEAIKALNVPVVGLIDAGTYSAGETFARDLAQIAGACLMGSTTAGA
                     SSSKRHWPLPNGFGTVVYAERSRWGIGGRPIEYNGIEPDETVEIVPEELQQGLNSGIL
                     RASERLDRVRQGGK"
     gene            5482..8199
                     /locus_tag="PK634_06610"
     CDS             5482..8199
                     /locus_tag="PK634_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW97445.1"
                     /translation="MRRLGLAFFLSGIGLALPAGAWDIRYSPVYPSIDQEIQIEIRGA
                     TQGAMLHWGVNAVGMIWDQAIPEYRPPGSILEGVATRTPMQGPDERGVLRLTLGPFNN
                     TNQLIRSLNFAIHWNDGTWANKDEKNYNIPVSFGRIGASPEEPTINDRVVVRVRRSRP
                     GGLLRWGVNAENQLWKPPRKEYWPPGTFATPDGLAVDTPLGKPDSNGVSTVALGPFNR
                     ADQVVRTLHMAVHWGDDWDTDAGRNYNVGISLETGPEAPSVRLLSPQDGEVIVDNPQV
                     QLQAEGADRAEMWLDGRSIASVGETPFELKIPFRQLKYGRHQLTARAEREGLVGLQEI
                     FFWKLPPHRVENLPQGVPWGATVNPDGTATFTLHAPGKKFISLVGDFNGWDPFADMMT
                     ASPDGTWWTTRPVSNGAPRYQFCIEGRQFLADPYARDIEWKDEAGREGHQPWHARAVL
                     EVGQPPFAWTATNYTRPPLDQLAIYEFHIDDLAPGGGFTGVIARLDYIRDLGFNAIEP
                     MPWMEFTVDRSWGYNPAFHFAPESAYGTPGELKRLIDEAHRRGLAVIMDAVFNHMDRN
                     SALYQLYGADYEASPYFRLFRGENWGFPDLDQQSRAFKRYVEDAIRCWLTEYRVDGIR
                     FDATRFVEWEGYNDWGAGWLAWAGRQADAGSYMIAEHIPSDPELIRRTEMDATWGDSF
                     RWVVRGYVEEGRLDRDEFARLLDPAQLGFGGVSERIAYTESHDEERVMRDLRRRGYSR
                     DEAERRALLALALTLTAPGPAMVYAGQEFGEDTAKTVGSNPLNWGRTEGWFGRPARRL
                     RDAARALVRLRTTHPALRTGAVTLDQQGLPDGVAVYTRGEAGAAVVVAANFGRRERRV
                     TVNLPGAGPWRDVLRGNPASPLRGDRITIALEPGGVAVWATDLSLASVR"
     gene            8273..9259
                     /locus_tag="PK634_06615"
     CDS             8273..9259
                     /locus_tag="PK634_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOW97446.1"
                     /translation="MKRTTPCRRWAALILLVMPLAAAGWGGRLHMDIGREAAQAAPDE
                     MKLWRMYARLVARYSVHPDLWKDYDPTEGERHYIDLEAYAPLTATNLPSDPTALRNGL
                     YRYHGHVPWVIMDLQSKLTAAMATGNWVQAASLAAAQAHYVGDLHQPLHTTENYDGQL
                     NGQNGAHMRWESDMPAIHWRSGLMPEAQAEYISNLWPAVLGWLDQSHGRAVDVLAADY
                     DALRVADPARDPETYHDYLWEATGDLFIEQVHRAACALASLWYTAWVDAGRPEIPPPP
                     ASLSEASVWVPEPQPEGMASWPFLAAFGVIAVVIVLLSMRRRPEPHRRDPSL"
     gene            9312..10052
                     /locus_tag="PK634_06620"
     CDS             9312..10052
                     /locus_tag="PK634_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOW97447.1"
                     /translation="MKTLLVTSRITFVPGNYDALVAGLAACPQIGGLLALDNRSRKLL
                     AKALGLAAGGAPRIGLQLLRNWFGPSARRRADAFAAAGKPAWTMPTIHSPEAVGLVRD
                     HGFDLVLNARTRFIYKADILSAPRLGCINIHHGLLPGQRGTMCDLWALFEGRPAGFSI
                     HVMIPQVDAGPILAREQVSDGTDRDYPAYLLKASRREREVAAGLLKDIEESGRFHASN
                     NSSIPGLALYRSPTFRQVWAMRRKGIRL"
     gene            10049..11761
                     /locus_tag="PK634_06625"
     CDS             10049..11761
                     /locus_tag="PK634_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOW97448.1"
                     /translation="MKDEERIGHQPGRAPLNNVISYIERHRAAFPERLALRWVETSDL
                     AAWDGRPASRLPHREISYSRFAEGIDRVAAGLKDLGLRSGDRAIIFLPMGVLMYTAMF
                     AVQRLGAVAVFLDSWARRHQLGASAACVQPKAMISHRAAFDLVAGLPEFGGLEIRIVA
                     GPGADASFTARLEDMLQRAGSVPVEAVEPEDTALVTFTTGSSGAPKGANRTHRFLCAQ
                     HEALASLLPYEPADADLPAFPIFSLNNLASGVTTVIPALDLAAPGPRDAALLATQLLR
                     EGVTCATLSPSMLVGVSRFCAEAGIELAGLRRVVTGGAPISRDNVRDFLRIAPRAACW
                     VLYGSTEVEPMAHIEAREMLAQAGKPDPEIVEEGVNVGRVSPELEYKFIRIRRGPIDL
                     KQESWAALEVPPGEVGEFIVTGDHVCRDYFNNEDAFRRAKIVDERGRVWHRTGDLARL
                     DPAGHLWIVGRIHNVIERGGRLCFPVRAEIILKQLPFVKQAAFLGLPDPGLGERTAAV
                     AALDPAGCDERRARDEILRLFAKNGIPVDDVFFVEAIPMDPRHHSKVEYDVLRKQLEG
                     GRRP"
     gene            11758..12654
                     /locus_tag="PK634_06630"
     CDS             11758..12654
                     /locus_tag="PK634_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOW97449.1"
                     /translation="MNSEARLFRAWMTFIRERFPPAANVVMAVFFSLGNAAVASRLAG
                     IPFPVWRWNMAAVVTVLFFFRLRCFDEIKDYATDLVLTPTRPLARGLLTLRQVRIMFG
                     VVTAAEFLLVAPLGFPALAAHAAAVVYSYLMYREFFVGAWLRPRLTLYAVAHTAVSIL
                     LGWSIAAQVLGPAHGKFPVSGLLFGIVNWMLFNVFEFGRKTFAPEEEKPGADSYSSLF
                     GPSGAVALCMSQVGIALLALARGPGRVVPQLALAAVLLAAGLVYAARATPATARAYRT
                     VSGVYLILFYLLVTVQAWPGGL"
     gene            12764..15433
                     /locus_tag="PK634_06635"
     CDS             12764..15433
                     /locus_tag="PK634_06635"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOW97450.1"
                     /translation="MIVSAQSVESFARREAGGKGFNLYRMGRLGLPVPEWVALGRGAF
                     ERFAAGGLRGRIEELVASLAPEQASAEIEKLILAAPMPETVSDEAARAWSLISGGGAI
                     SVRSSAADEDGAAHSFAGQLSSFLYVASLDDALRCLRRCWASAFSARALAYRRERGLP
                     SRGIGIAVIFQRMVDPKVSGVLFTCDPAAKDPSRYVVSAVYGAGEGLVSGALDADTTW
                     LDAATGAPARRVIALKTEQFRRGPSGECAREAVDPDRREQPCLDDARLAELYRLGRTL
                     HDYFGRPQDIEWALAGGQLCLLQTRPVTSLDENAAGYPNLWDNSNIVESYGGLTLPLS
                     FSFALRNYRGVYIQFCEVLGVPREVIKDMEPTLAHMLGCVNGRVFYNLYNWYKLVGVL
                     PGFRRNREFMETMMGVRESLAPEIADRIRPHPSWDTLAGRGRRLVTGLSFLVHHFRIQ
                     RLVDRFLRDFDREYREFRHRPYDRMRSDEIFNVYLEMERRMMSRWKAPIINDFLVMVH
                     FGLFRKLTARWLGETDPNLQNDLLAGEGHMESAEPTRRLIELAGRAAAQPDLRALIER
                     TPEADLLEALNQSAFKEFFGEVRDYLDRFGFRCMNEMKLEEADLFTDPSFFFACLKNY
                     LRGGTTDLAALDRRRAELRERAEALVAGRLAGWRLRLHRWSMKHARKAVRNRENTRFA
                     RTRVYGVARTMFQAMGEDLCGQGLLERPRDIFYLEMDEISGLHMGTLTAYDLKAVVAL
                     RKKAYAEFAAAEPKTRFQTRGPVYWRNRFRAEPETPDRPADGSADLRGLPCCPGVVEG
                     VVKVVQAPGDDLKLNGEILVAPRTDPGWVPLYPSVSGLLVERGSLLSHSAIVAREMGL
                     PAIVAVRGLTATLSSGMRVRMDGQAGTIKILDR"
     gene            15519..16820
                     /locus_tag="PK634_06640"
     CDS             15519..16820
                     /locus_tag="PK634_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HOW97451.1"
                     /translation="MPAPRKIAAEILNHLLAGTKAGDEVHRLARRPPEKEPLPPHVPH
                     GNDHTAAGLEKRRAFLKAQGFCIEQLSGLGPDPAPESLEGHIENFVGYAKVPVGVIGP
                     LRVNGAHAHGDFYVPLATSEGALVASYNRGAYVISQSGGAAAMCLTESVSRSPCFAFR
                     SLADAGLFLAWTLPRFESFQKVVATTSGHARLLDMRTVMLGKELYLILDFTTGDASGQ
                     NMVTLAADALARHLVGSAPVKPERWYVEGNLSGDKKATMLSFLFARGKKVVAEAVVRR
                     DLLKKVIHITVDEMLAGWQVSILGGIQSGAIGAQGHFANALAALFIACGQDAACVAEA
                     SVGLTRMDKTADGDLYVSISLPNLIVGTVGGGTTLPGAREGLAMLGCAGEGGARKFAE
                     ICAVTALAGEISLTGAIIAGEFSKAHARYGRKRKAEKPDGG"
     gene            16810..17892
                     /locus_tag="PK634_06645"
     CDS             16810..17892
                     /locus_tag="PK634_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HOW97452.1"
                     /translation="MADDLAQRVRFDFIHYANCWEDPRLLFEALQPVGKRILSIASGG
                     DNSLALLGEGAEVVAADLSRAQLACIELKVAAIRELDDEALLAFLGLRPSADRLAVHD
                     KLLRLRLSENAAAFWNGHPGDIAAGIVHAGKFENYFRLFRTRVLPLIHGRRTVESLLA
                     ARDAAGRRDFYDRRWNNRRWRLMFRLFFSRWVMGRFGRDPEFFRYVEGSVADRILARA
                     EHALTVLPTHDNPYLEYILTGNFARALPPYLEPERLARVRANLDRLTLFEGPAQEAPG
                     EFDGFNLSDIFEYMPPDVAERVYGQLLDKARPGARLAYWNMLVPRACPEPYRARVKAL
                     DELGARLLEQDRAFFYRRFVVAEARA"
     gene            17889..18371
                     /locus_tag="PK634_06650"
     CDS             17889..18371
                     /locus_tag="PK634_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97453.1"
                     /translation="MKARLTNKRRFSLTRRDAELFRLARRADHRTLAIWARDCAKRVM
                     PYFESARPADPRPRQALEALQAWIDTGQFKMAVIRKASLSSHAAARAVGEDNAARSAA
                     RAAGQAVATAHVQTHAPGAALYAQQARHRAADPAEADAAAASERDWQLAHLRKRMGKE
                     "
     gene            18379..19767
                     /locus_tag="PK634_06655"
     CDS             18379..19767
                     /locus_tag="PK634_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97454.1"
                     /translation="MEESSSWWAGGAADLWGALLFGALFLPLLGVAEWLRRRGSFGPE
                     ATRKLVHIGGGLLSLALPGLVRSPAVVLAMSAALSLLFLWAKRRAALASLHGVARRTS
                     GTEYFPLAVFLVYVATYGQYWLYACSMLVLAVADAFAALIGSRYGRHRYEVEDEVKSV
                     EGSGVFLLIAFLAILLPMLLLTSLPRVVCVLSALLVAVLVTGFEAVSMGGADNLFIPL
                     GVAVILAKITTKPVPEIVYQNASLAALSLGLALAARRTPSFNAGGMILFALFVFGAWS
                     LGSERWALPVLAAFVVYAGARTFIPGPGGAPARVRVRILFRVLVIPLLLLVTGNMLRR
                     GPEFYAPFVTAMAVVLAFGLWNHVLWARRPAGARRRALLWLAALAAWAVEIVPAWLLL
                     PGRPATSGLWLPAGLVLAASALDDLLLGPDPSFEGERAWPAWRLVLAAAAGAAVLLAQ
                     RAGGLAPWPYPP"
     gene            19849..20262
                     /locus_tag="PK634_06660"
     CDS             19849..20262
                     /locus_tag="PK634_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOW97455.1"
                     /translation="MKKYMPRELAGLAAKIRRFNRHRDWDKYHAPKNLAMGVMIEAAE
                     LAEHFLWQETAESRRPDARARAAIRDEVGDVLILLLNLCDKLDIDPVAAALNKLEHNR
                     RKYPAALVRGKALKYDQYPERAARGTKRRPPLSVR"
     gene            20466..20732
                     /locus_tag="PK634_06665"
     CDS             20466..20732
                     /locus_tag="PK634_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97456.1"
                     /translation="MTGSAQFGTAGLMTYHQVVYVPNISFSARYNGSIEPPRFLSLVE
                     LPVIDSMQRDGKLSFSGVATSTVASVEWAASLAEPGRTNGVSAP"
     gene            complement(21013..22287)
                     /locus_tag="PK634_06670"
     CDS             complement(21013..22287)
                     /locus_tag="PK634_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOW97457.1"
                     /translation="MVPEELVAGGTGTSLAVTSAGEPHVLTVCSGVVVHAARTGGLWT
                     NTPIRAGDAASLCFMNDEAAYLAYSAREYDGDTYHEVVLAVMATGSVYDLGMLLDGSL
                     STYTCAGRAANEHHDYVNPVLRCADGVIHVAARHQRDRDASYKPGGVFCPQTHDERIE
                     YIRIEYPLTQIASSEWSYYHHVFLRDSCLCMTTGASACLVFTAGPAAYLSPCSIPWSE
                     TSLGFRQVSAVDFSSDGLLGTFADDTPSWFQVMTNGARDHLYEIGATSPGWNMDVCME
                     HMALLFVDDQDAVSNEVFLILRLDRDEDGLDDRWEYHFFGSPTNGLPGKDSDEDSFTD
                     AEEQAAGTDPTNGMSFFRLEAFAPSDLPSCVLSWQGRTGRSYTLQGSADLPAPSETLA
                     EVSLIQDDRVFITNAPPESATRRLYWLTVDRP"
     gene            complement(22688..>22923)
                     /locus_tag="PK634_06675"
     CDS             complement(22688..>22923)
                     /locus_tag="PK634_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOW97458.1"
                     /translation="AERMVVELRDKLTAGEALEAVAGAAAGEPDARLRDAILALVSLG
                     YKQADAQKRVRDVLPGVGPKTTVEEIVRKALAP"
BASE COUNT         3436 a         7761 c         8035 g         3691 t
ORIGIN      
        1 tgctttactc tggcccaagt acggcggttc gcacgaggtt caagagacac cgccatttcg
       61 atccgagcca aactcgccgg ggaggcgcta tggacgggat aacccttctg attgttgcgg
      121 ccgtcgtcgt cgggctgttc ctgctcgtga cgttcaacac gctggtcgga cgccggaaca
      181 aggtccggaa cgcgttcggc accgtcgacg tgctgttgaa gaagcggtac gacctgatcc
      241 cgaacctcgt ggcgaccgtg cagggctaca tgcagcacga gcggacggtg ctgcagcaga
      301 tcacggacct gcgcagccgc gcgatgggcg ggccattgcc gccggaacag ctctttcgcg
      361 tgaatgcgca actggggcaa ctgctcggcg gagtgcgcgc cgcgatcgaa aactatcccc
      421 agctgaaggc gagccagaac gcgctccagt tgcaggccgc gctgaacgag gtcgaagagc
      481 aaatttccgc cggccgccgg gcgtataacg cggccgtgac ggacttgaac aacgccctgg
      541 aaatgttccc gtcgaacctc gtcggttcgc tcttcggatt ccagaaggcc gtgttcttcg
      601 aggccggcga ggcggagcgg ggcgccgtgc cggtccagct tggcggcgcc ccgtgacatc
      661 ggagccgcca tcggccgctg attccgcggc ccccggctcg ccggtccgca ccgaggagga
      721 attccgggcg ttctaccagg gcgtcctgct cccggagctg gagagcgtcg accgggagcg
      781 cagggccgtg cggggccgcg tcctggttct gtggctttcc ctgctcgggc tgattttcct
      841 ctcgctgctc attttcggcg ggctgcaggc gtcgggcccc ccgggcgcgg cggtcgtggt
      901 ggtggtcggc gggatcggcc tcgcgctgtt cggattctgg ctggccgcct tcgcgcggct
      961 ttcccgggca aagcgggcgt atatcgcccg ctacaagaac acggttatcg gccacatcat
     1021 ccggttcatc gaaccgggcc tggcgtacga gcccgactcc ttcgtggccg aggcggtgta
     1081 ccagaagagc ggcctgttcc cgcgcgcgtg ggaccggtac cgcggggacg atttcatcca
     1141 tggccgcgcg ggccgcaccc cgctgttctt ctccgagctg cacacggagt acaagacgcg
     1201 gcagcgcacg tcggacaacc gcacccggga aacctggcac acgattttcc acggcctgtt
     1261 cttcgtgtgc gaattcaaca agacgttcca ggggcgcacg gtcgtcctgc cggaccggct
     1321 ggagggcctg ctcggcgacc tgggcgcggc gcttcagttc agccggtccc agtacggcga
     1381 gctggtcaaa ctggaagacc ccgagttcga gcggatgttc gccgtctacg ggtcggacca
     1441 ggtcacggcg cgctacattc tctccaccag cctgatggcg cgcctgatgg ccttccgggg
     1501 caaggcgcgg caggacatcc gactcgcgtt cgtggactcc aacctgtacg cggcggtctc
     1561 gttcaaccgc gacctcttcg agccgcgcgt ctggcgctcg ctgatcgact acgaggagat
     1621 ccgcggctac tttgaaatcc ttcagctcgt gatcggcatc gtcgaggact tgagcctcaa
     1681 cacgcgcatc tggggcgagc gggccctctc cggcgcctga ccgggcgcgc tacgccgggc
     1741 gcagttcgag gaccgcgatc tcgggcaagc agttgaaccg gaccgggagg taggcccatc
     1801 cgatgccccg ggagatgaat acgcccagcc cgcgcggcga acggcagagc ccgctggaat
     1861 agcgcttgtt tcgcaccggc aggatggggg ccccgaggaa cgggaggcgc acctgcccgc
     1921 cgtgggtatg cccggagatc atgagatccg cgcggccggc gaagggcgtg tcggcggtgt
     1981 cgggattgtg ggccaggacg atccggcagc cggcgtccgg gatccgcgcc agcagatcgt
     2041 cgaggcgccg gtggtcctcc catagatccc cggcgcccgc cagccacagc tgctgcccgt
     2101 ccttttccag ggccgcgacc ctgtgccgca ggccctgccc ggtctgctcg agccagtgct
     2161 ccgaccgcgc ggtgtcggcc cagtggtcgt ggttgccgag gacggagtgc accccgagcg
     2221 gcgcggagag ccgggcgagc tcgggccaga cggcgtcgat ctggcgcgcg gcattccgct
     2281 cgtgcacgta atcccccgtg cagacgatca ggtctccgcc gagttcgttg gcgcgccgga
     2341 tcacgtcggc aatcacccgc atcggcatca gcgcgccgaa gtgcaggtcg ctcaagtgaa
     2401 ccacccgcag gccggcgaag gccggcggca gccgcggcac gggaatccgg taggtgttcg
     2461 tggcaaccag gtagcgctcg atgaaaaacg ggtagctgga gaccagcccg atcgcgccga
     2521 gcgggccggt catcttcaga aatcggcggc ggctgatcgc cgtggtcatg cccctatttc
     2581 ccgccctggc ggacgcggtc caggcgctcc gaggcgcgga ggatgccgga gttcaggccc
     2641 tgctgcaatt cctccggcac gatctccacc gtttcgtcgg gctcgatccc gttgtactcg
     2701 atcggccggc cgccgatgcc ccagcggctc cgctcggcat agacgaccgt gccgaacccg
     2761 ttcggcaacg gccagtggcg ttttgatgac gacgcccctg ccgtcgtcga gcccatcagg
     2821 caggcccccg cgatctgcgc caggtcgcgc gcgaaggtct cgcccgcgga ataggtgccg
     2881 gcgtcgatca ggccgaccac gggcacgttc agggccttga tggcctcggc cgaggcgcgg
     2941 ccgcccccgc ccccgccgca gccgcgcagg tcgacgatca ggcccggcgc gccctggaat
     3001 cccttcacga tgtcgatgaa cgccgcctcg tcctgcatgc gccggcacca ggcgtaaccg
     3061 tacccgttgc tcaagacggc atggaagacg cccggcccct cccggaggcc gggcggaagg
     3121 gcataggcgt ggggccagcc ccgcgtttcc cggtcggccg tccgcttcag cgtcgcgggt
     3181 tcggtcgttc ccgcggggac atattcgatt tcgaactcgg gcggtttctc gaaatcgccc
     3241 tcttcccagc ggaagccgta gcggtaggcg aacagccggg cccgctggcg cgagggatac
     3301 cctccctttt cccagagaga ttccgcctgc tcctcgaacc aggcgcgggc cgggctcccg
     3361 ttgaccttcg tgacgaccgt gcccggcggg agcgttgcgg cgagttcggg aaaggattcc
     3421 atgaccacga cgcggtcttc cgtggccggg tggaacgaga cgccgccgta gcgggtctcc
     3481 gggaatgccg gctccacgcg cggatccgcg atatgcatgt gcccgtcgcg caggacgagg
     3541 atcgccgcga gcatcacgcg catgaattcc gcgtcattcg tgcaggcggc ggcctcggct
     3601 gccaggttga ccttggccgc ctcccagttc ccgcggatgc ccttgaggtc gaagaaggga
     3661 tagtgcgcgt ccatttcctt cgcgaaagcc gagaaggcgc cggcgtagtc gttggcctcg
     3721 agcgggcagg cctcgaggtt caggaggcgc aagagcccct cggcctccgc ggccgcggac
     3781 ggcgtcgtgt ccgcggcgtc gttcgaaatg gcctgcaggg tctcccggat ggattttttc
     3841 acgtcgacag ggaggctctt catagccggc ggcgtgaggc ggcgcaggag cgcgacttgt
     3901 tcggcgggtg gccgcgcgcc cagggcgtcc cccagttctt ccagcggcat cagcgccagg
     3961 acgaccggcg tgttggaggc aagggccatc gcgccgaggt agccctcctt ttccgcgaac
     4021 gccggttgcg ccctggaggc gggcggaacg cccagttcga agcggccctc cggcccggag
     4081 gtcgtggtga cggcccaggg agattcgccg atcaggggct ccagcgacga caggtaaaac
     4141 cttggcggcg cgccaaattc ctcgtcgaga atacgggccg ggagcagcac ggccgtgatc
     4201 cggcattgcg gcagggggcg gccctgcgcg tccgtcgcga cgcccgccag ccgcgcgcgg
     4261 ttggtcgtca gcgtgaaatc atatgcgcgg ccgccttcga tcccttcctt catttgcggc
     4321 gcccgcgccc cggacggatc cacgacggcc aggaacccgc ccgcgtagcg caggggctcg
     4381 gtttcaaagc gcccctccgc gtccgtcgtc atccggtgat ccccgcccac gtacgggtag
     4441 ccgtagtaca ccgggatgcc cgcgagcggg cgtccttccg catccgacac cgtccccctc
     4501 gcgggcctgg gcggggacag aaggaacagg gcctggcggg ccaactccgg cgtcacgagg
     4561 gcgtgcgatt cccggccgtc ggcgatggcc tgcactctcg gcaacagggc gtccagcgtt
     4621 tccgcgccca gccacggcgg gtccagcgcg tggaggcagc gaaggtactc cgcgcccatc
     4681 gggcctccca gcgttttaag ctcccgccgg atgtcctccc gggcatagac cgtcaggacg
     4741 cacggcgacg tggcgacggg gaaggccatg tcgaggtagc cgtccttgat cacctcgacg
     4801 aggcggggcg tggccgtaaa ggcctcgacg gaaaattcgc ccttcgcgtc cgtcacggac
     4861 gagcggctcc agcggaccat gtgctctcct ccgccgtagc gtcgccggga atccgcgtct
     4921 tggagcagct tccccgacgc caggtagacg gtcacggtgg cgccggcgac gggctcgccc
     4981 gcctcgtcca cgacgcgtcc ggcggtcgcg ccccaatccg gccgcatcac gatgtcgtgg
     5041 gaccgccctg cgatggcggg ttccacgagc gcgggcgcga ggcgcccgga cggatccacg
     5101 gccgccacga cctgctccca cggggggagc cgggtgaact cgaagcgccc gtccgcgtcc
     5161 gtcgcggcct cccagccgga ggagttcacg taggggagat agcagtggac ggcgatcccg
     5221 gcgagcgcgg ccccgattcc gtccagaacg cggccggtca ttttatgcgg cgcgggcgag
     5281 ggggctgcgg gttcagcggc cgggccgtca tggcccagca tcaggctcgt tgcgagcaac
     5341 aggatgaatc gtgtgttcat tgccctgtat agacagccgc cggcccgaat agtctcacgg
     5401 ggatctgcgg tctttttcgc gggcgctttt tttcattctc ggctggccgc gcggcggggc
     5461 gaccgggtaa agtgccggga catgaggcgc ctcggtttag cgtttttcct ttcgggcatt
     5521 ggcttggcgc tcccggccgg ggcctgggat atccggtaca gcccggtcta cccgtccatt
     5581 gaccaggaaa tccagatcga gatccgcggc gcgacgcagg gcgcgatgct gcactggggc
     5641 gtcaacgccg tgggcatgat ctgggatcag gccatccccg agtaccgccc gcccggctcc
     5701 attctggaag gcgtggccac gcggacgccc atgcaggggc ccgacgagcg cggggtgctg
     5761 cggctaaccc tgggcccgtt caacaacacg aaccagctca tccggtcgct gaatttcgcc
     5821 atccactgga acgacgggac ctgggcgaac aaggacgaaa agaactacaa catcccggtc
     5881 tccttcggcc gcatcggggc cagcccggag gagccgacaa tcaacgaccg cgtcgtcgtc
     5941 cgcgtccggc gcagccggcc gggcgggctg ctccgctggg gcgtcaacgc ggagaaccag
     6001 ctgtggaagc cgccgcggaa ggagtactgg ccgcccggca ccttcgccac gccggacggg
     6061 ctggccgtgg acacgccgct cggcaagccg gacagcaacg gcgtctccac cgttgcgctc
     6121 ggcccgttca accgcgcgga ccaggtcgtc cgcacgctgc acatggccgt gcactggggc
     6181 gacgactggg acaccgacgc gggccgcaac tacaacgtgg ggatttcgct ggagaccggc
     6241 ccggaggcgc cctcggtccg cctgctctcg ccgcaggacg gcgaggtcat cgtggacaac
     6301 ccccaggtcc agctgcaggc cgagggcgcg gaccgggcgg agatgtggct ggacggccga
     6361 tccatcgcgt cggtcggcga gacgcccttc gagctgaaga tcccgttccg ccagctgaag
     6421 tacggccggc accagctcac ggcgcgcgcc gagcgcgagg ggctcgtggg cctgcaggag
     6481 atcttcttct ggaagctgcc gccgcaccgc gtggagaacc tgccgcaggg cgtgccgtgg
     6541 ggcgcgaccg tcaacccgga cggcacggcg accttcacgc tccacgcccc gggcaagaag
     6601 ttcatatcgc tcgtcggcga tttcaacggc tgggacccgt tcgccgacat gatgaccgcc
     6661 tcgccggacg gcacgtggtg gacgacgcgg ccggtctcga acggcgcgcc ccggtaccag
     6721 ttctgcatcg agggccggca gttcctggcc gatccctacg cccgcgacat cgagtggaag
     6781 gacgaggccg gccgcgaggg ccaccagccg tggcacgcgc gcgccgtgct ggaggtcggc
     6841 cagccgccgt ttgcctggac ggccacgaac tacacccggc cgcccctcga ccaactggcc
     6901 atctacgagt ttcacatcga cgacctggcg cccggcggcg ggttcaccgg cgtgatcgcc
     6961 cggctggatt acatccggga cctgggcttc aacgccatcg agccgatgcc gtggatggag
     7021 ttcacggtcg accggagctg gggctacaac cccgcgttcc acttcgcgcc cgagtcggcg
     7081 tacggcacgc ccggcgagct caagcggctg atcgacgagg cgcaccggcg gggcctggcc
     7141 gtgatcatgg acgcggtctt caaccacatg gaccgcaact ccgcgctcta ccagctctac
     7201 ggcgccgact acgaggccag cccgtacttc cgcctgttcc gcggcgagaa ctggggcttc
     7261 ccggacctgg accagcagtc gcgcgccttc aagcgctacg tcgaggacgc gatccgctgt
     7321 tggctgacgg agtaccgcgt ggacgggatc cgcttcgacg cgacgcgctt cgtcgagtgg
     7381 gagggctaca acgactgggg cgcgggctgg ctggcgtggg cgggtcggca ggcggacgcg
     7441 ggcagctaca tgatcgccga gcatatcccc agcgatcccg agttgatccg gcggacggag
     7501 atggacgcca cgtggggcga ctcgttccgc tgggtcgtcc gcggttacgt cgaggagggc
     7561 cgcctcgacc gcgacgagtt cgcccggctg ctcgacccgg cgcagctggg cttcggcggc
     7621 gtgtcggagc gcatcgcgta caccgagagc cacgacgagg agcgggtcat gcgcgacctg
     7681 cggcgccgcg gctactcccg cgacgaggcg gagcgccgcg ccctgctggc gctggcgctg
     7741 acgctgacgg cgcccggccc cgccatggtg tacgcgggcc aggagttcgg cgaggacacg
     7801 gccaagaccg tcgggtccaa cccgctgaac tgggggcgca cggaaggctg gttcggccgg
     7861 cccgcgcggc ggctgcggga cgcggcgcgg gcgctcgtcc ggctgcggac gacgcacccc
     7921 gcgctgcgca ccggcgcggt gaccctcgat cagcagggcc tcccggacgg cgtggcggtc
     7981 tacacgcgcg gcgaggccgg ggcggcggtc gtcgtggccg cgaacttcgg gcggcgcgag
     8041 cgccgcgtga cggtgaacct gccgggcgcc gggccgtggc gggacgtcct gcgcgggaat
     8101 cccgcgtctc cgctccgggg cgaccggatt acgatcgcgc tcgagcccgg cggcgtggcc
     8161 gtctgggcga ccgacctctc gctggcctcg gtccgttgac cgcccgcttc ttccccgcga
     8221 aggcggcttg acctttccgg aatccgggcg tacgtagggc agggcttggc gaatgaaaag
     8281 aacgactcca tgccgccggt gggcggcgct gattctcctg gtgatgccgc tggcggcggc
     8341 ggggtgggga ggccgcctgc acatggacat cggccgcgag gcggcccagg ccgcgccgga
     8401 cgagatgaag ctctggcgca tgtacgcgcg gctggtggcc cgctacagcg tgcatcccga
     8461 cctgtggaag gactacgatc ctaccgaggg cgagcggcat tacatcgacc tcgaggccta
     8521 tgccccgctg accgccacca acctgccgtc cgatccgacg gcgctgcgga acggcctgta
     8581 ccgctaccac gggcatgtgc cgtgggtcat catggacttg cagtcgaaac tgaccgccgc
     8641 catggccacc gggaactggg tccaggccgc cagcctggcc gcggcccagg cgcactacgt
     8701 gggcgacctg caccagccgc tgcacaccac ggaaaattac gacggccagc tcaacgggca
     8761 gaacggcgcg cacatgcgct gggaatccga catgcccgcc atccactggc gcagcggcct
     8821 catgccggag gcgcaagcgg agtatatctc caacctctgg cctgccgtcc tgggctggct
     8881 ggaccagtcg cacgggcggg ccgtcgacgt cctggccgcg gactacgacg ccctgcgggt
     8941 ggccgacccg gcccgggatc ccgaaacgta ccacgactac ctgtgggagg ccaccggcga
     9001 cctgttcatc gagcaggtcc accgggcggc ctgcgccctg gccagcctct ggtacacggc
     9061 ctgggtggac gccgggcggc cggagatccc gcctccgccg gccagcttga gcgaggcttc
     9121 cgtctgggtg ccggagcccc agcccgaggg catggcctcc tggccgttcc tggcggcgtt
     9181 cggcgtcatc gcggtcgtca tcgtgctgtt gagcatgcgg cgccgtcccg agccccaccg
     9241 gcgcgatcct tcgctctgac gggccggcac atccggcgtg caattatcgc gcgcgcggct
     9301 acactggccg cgtgaagacg ctgctggtca cctcgcggat caccttcgtc cccgggaact
     9361 acgacgcgct cgtggcgggg ctggccgcgt gcccgcagat cggcgggctg ctggcgctcg
     9421 ataaccggag ccgcaaactc ctcgccaagg cgctggggct ggccgccggg ggcgccccgc
     9481 gcatcggcct gcaactcctg cgcaactggt tcggtccgag cgcgcggcgg cgggcggacg
     9541 cttttgccgc ggcaggcaaa ccggcgtgga cgatgccgac catccattcg cccgaggccg
     9601 tcggccttgt ccgcgatcac ggattcgacc tcgtcctgaa cgcgcggacg cgcttcatct
     9661 acaaggccga catcctgtcc gcgccgcggc tcggctgcat caacatccac cacggcctgc
     9721 tgcccgggca gcgcggaacg atgtgcgacc tctgggcgct tttcgaaggg cggcccgccg
     9781 gcttctcgat ccatgtcatg atcccccagg tcgacgcggg acccatcctc gcccgcgagc
     9841 aggtctcgga cggaaccgat cgtgattacc cggcctacct gctcaaggcc agccgccggg
     9901 aacgcgaggt cgccgccgga ctcctgaaag atatcgaaga atcgggccgc ttccatgcct
     9961 ctaataatag ttccatccca gggctagcct tataccgaag ccccaccttc cgacaggtct
    10021 gggccatgcg cagaaaaggg atacggctgt gaaggacgag gaacggatcg gccatcaacc
    10081 gggtcgcgcg ccgctgaaca atgtcatcag ttacatcgag cggcatcgcg ccgcgtttcc
    10141 agagcggctc gcgctgcgct gggtggagac ttcggatctc gccgcgtggg acggtcgtcc
    10201 cgcctcgcgc ctgccgcacc gggaaatttc ctacagccgg ttcgcggagg gtatcgaccg
    10261 cgtggcggcc ggcctgaagg atctcggact gcggagcggc gaccgcgcga tcattttcct
    10321 gccgatgggc gtgctgatgt acaccgccat gttcgccgtg caacggctcg gggccgtcgc
    10381 ggtcttcctc gactcctggg cgcgccgcca ccagctgggc gcgagcgcgg cctgcgtgca
    10441 gcccaaggcc atgatcagcc accgcgccgc cttcgacctg gtcgccggcc tgccggaatt
    10501 cggcgggctc gaaatccgga tcgtcgcggg gccgggcgcc gacgcctcgt tcacggcgcg
    10561 gctggaagat atgctccagc gcgccggctc cgtccccgtg gaggccgtgg agccggagga
    10621 taccgccctc gtcacgttca ccaccggcag ctcaggcgcg cccaagggcg cgaaccgcac
    10681 gcacaggttc ctctgcgcgc agcacgaggc gctggcctcg ctcctgccct acgagcctgc
    10741 ggacgcggac ctgcccgcgt ttccgatctt ctcgctcaac aacctggcct ccggagtgac
    10801 cacggtcatc ccggcgctcg atctcgccgc gcccggcccg cgcgatgccg ctctgctcgc
    10861 gacgcagctc ctgcgcgagg gcgtgacctg cgccacgctg tcgccctcga tgctggttgg
    10921 tgtgtcgcgc ttctgcgcgg aggcggggat tgaactcgcc ggcctccgac gcgtggttac
    10981 cggcggcgcg ccgatcagcc gggacaacgt ccgcgacttc ctgcgtatcg cgccgcgcgc
    11041 ggcctgctgg gtcctctacg gctcgaccga ggtcgagccc atggcgcaca tcgaggcgag
    11101 ggagatgctg gcccaagccg ggaagcccga ccccgagatc gtcgaggagg gcgtcaacgt
    11161 cgggcgcgtc agcccggaac tggaatacaa gttcatccgg atccggcgcg gcccgatcga
    11221 cctgaagcag gaaagctggg cggccctcga ggtcccgccc ggcgaggtcg gcgagttcat
    11281 cgtcaccggc gaccacgttt gccgagacta cttcaacaac gaggacgcgt tccgacgggc
    11341 caagatcgtg gacgagcgcg gccgggtgtg gcaccggacc ggcgacctgg cgcgcctgga
    11401 cccggcgggc catctctgga tcgtcggccg gatccacaac gtcatcgagc gtggggggcg
    11461 cctgtgcttc cccgtgcgcg cggagatcat cctcaagcag cttcccttcg taaaacaggc
    11521 ggcttttctt ggcctgcccg atcccgggct cggggagcgc actgccgccg tcgcggcgct
    11581 ggaccccgcg ggctgcgatg agcgaagggc tcgcgacgag atcctccgcc tcttcgccaa
    11641 gaacggcatc cccgtggacg acgtattctt cgtcgaggcc atccccatgg acccccgcca
    11701 ccattcgaag gtggaatacg acgtgttgcg gaagcagttg gagggcggcc ggaggccatg
    11761 aacagcgagg cccgtctttt ccgcgcctgg atgacgttca tccgcgagcg cttcccgccg
    11821 gcggcgaacg tcgtgatggc ggtctttttc tccctgggca acgcagccgt ggcgtcgcgg
    11881 ctcgcgggga ttccgttccc agtgtggcgc tggaacatgg cggcggtggt gacggtgctg
    11941 ttctttttcc ggctgcggtg tttcgacgag atcaaggact acgcgacgga tctcgtgtta
    12001 accccgaccc gcccgctggc gcgcggcctg ctgaccctgc gccaggtgcg gatcatgttc
    12061 ggcgtggtca cggcggcgga attcctgctc gtcgcgccgc tgggcttccc cgcgctggcg
    12121 gcgcacgcgg cggcggtcgt gtacagttac ctgatgtacc gcgagttctt cgtcggcgcg
    12181 tggctgcgcc cgcggctgac gctctacgcg gtggcgcaca ccgccgtctc gatcctgctc
    12241 ggctggtcca tcgcggccca ggtcctcggc cccgcgcacg ggaagttccc ggtgtccggg
    12301 ctgctcttcg ggatcgtgaa ctggatgctg ttcaacgtgt tcgagttcgg gcgcaagacc
    12361 ttcgcgccgg aggaggaaaa gcccggggcg gacagctact cgtcactgtt cggcccgtcg
    12421 ggcgccgtgg cattgtgcat gagccaggtc gggattgcgc tgctggcgct ggcccggggg
    12481 cccgggcgcg tggttcctca actggccctc gcggcggtcc tgctggcggc gggactcgtt
    12541 tacgcggcgc gcgcgacgcc ggcgacggcg cgcgcgtacc ggacggtatc gggtgtatac
    12601 ttgatcctgt tctacctgct ggtaacagta caggcctggc cgggaggact ataaacttcg
    12661 tggtagggcg cgctggccca gcgcgccgcg aacggcggct tgagccaagc cgccctaccc
    12721 tggggcgctt gccgatgaag tcaggcgtgg ggggaatagc ggaatgatcg tgagcgcgca
    12781 atccgtcgag tccttcgccc ggcgcgaggc cggaggcaag gggttcaacc tctaccggat
    12841 gggccggctc ggcctgcccg ttcccgaatg ggttgcgctc gggcgggggg ccttcgagcg
    12901 cttcgccgcc ggcggcctgc gcggccggat cgaggagctc gtcgcctccc tcgcgccgga
    12961 gcaggcctcc gccgaaatcg aaaaactgat tctcgccgcg ccgatgccgg agaccgtgtc
    13021 cgacgaggcc gcgcgggctt ggtcgctcat ctcgggcggc ggcgccatct cggtgcggtc
    13081 ttccgccgcg gacgaggacg gcgccgcgca ctcgtttgcg ggccagctgt caagcttcct
    13141 ctacgtggcc tcgctggacg atgcgctccg ctgcctgcgg cgctgctggg cctcggcgtt
    13201 ctccgcgcgg gcgctggcct accggcgcga gcgcggcctg ccgtcccgcg gcatcgggat
    13261 cgcggtgatc ttccagcgca tggtggaccc aaaggtttcc ggcgtgctgt tcacgtgcga
    13321 cccggcggcg aaggatccgt cgcgctacgt cgtcagcgcc gtctacgggg cgggggaggg
    13381 cctggtctcg ggcgcactgg acgcggacac gacctggctg gacgcggcca cgggcgcgcc
    13441 cgcgcggcgg gtgatcgcgc tgaagaccga gcagttccgg cgcgggccgt cgggcgagtg
    13501 cgcgcgcgag gcggtggacc cggaccggcg ggaacagccg tgcctggacg acgcgcggct
    13561 ggcggagctg taccgtctgg gccggacgct gcacgactac ttcggccgcc cgcaggacat
    13621 cgagtgggcg ctggccgggg gacaactgtg cctgctgcag acccggccgg tcacgtcgct
    13681 cgacgagaac gcggcgggct acccgaacct ctgggacaac tcgaacatcg tcgagagcta
    13741 cggcgggctg acgctgccgc tgtcgttctc cttcgccctg cgcaactacc gcggcgtgta
    13801 catccagttc tgcgaggtgc tcggcgtgcc gcgcgaggtg atcaaggaca tggagccgac
    13861 gctcgcccac atgctcggct gcgtgaacgg gcgcgtgttc tacaacctgt acaactggta
    13921 caagctcgtc ggcgtcctgc ccggcttccg ccgcaaccgc gagttcatgg agaccatgat
    13981 gggcgtgcgc gagtcgcttg cgccggagat cgcggaccgg atccggccgc acccgtcgtg
    14041 ggacacgctc gcggggcgcg gacggcggct ggtcaccggc ctctcgttcc tcgtccacca
    14101 cttccgcatc cagcgcctcg tggaccggtt cctgcgcgac ttcgaccgcg agtaccggga
    14161 attccggcat cggccctacg accggatgcg cagcgacgag atcttcaacg tctacctcga
    14221 gatggagcgg cgcatgatga gccggtggaa agcgccgatc atcaacgatt tcctcgtcat
    14281 ggtgcacttc ggcctcttcc ggaagctcac ggcgcggtgg ctgggcgaaa cggatcccaa
    14341 cctccagaac gatttgctcg cgggcgaggg gcacatggag agcgccgagc cgacccgccg
    14401 gctgattgag ctcgccggcc gcgccgccgc gcagcccgac ctgcgcgcgt tgatcgagcg
    14461 gaccccggag gccgacctgc tcgaggcgct gaaccaaagc gcgttcaagg agttcttcgg
    14521 cgaggtgcgg gactatctcg accgcttcgg gttccgttgc atgaacgaga tgaagctgga
    14581 ggaggcggac ctcttcaccg atccgtcgtt cttcttcgcc tgcctcaaga actacctgcg
    14641 cggcggcacg accgacctcg ccgcgctcga ccgccgccgg gccgaactgc gcgagcgggc
    14701 cgaggcgctc gtggcggggc ggctggccgg ctggcgcctg cgcctgcacc gctggtccat
    14761 gaagcatgcg cggaaggccg tgcgcaaccg ggagaacacc cgcttcgccc gcacccgcgt
    14821 ctacggcgtc gcgcgcacga tgttccaggc gatgggcgag gacctctgcg ggcagggcct
    14881 cctcgagcgg ccgcgcgaca tcttctatct cgagatggac gaaatctccg gcctgcacat
    14941 gggcacactg acggcgtacg acctcaaggc cgtcgtggcg ctgcgcaaga aggcctacgc
    15001 ggaattcgcg gcggcggagc cgaagacgcg gttccagacg cgcggtcccg tctactggcg
    15061 caaccggttc cgggcggagc ccgagacccc ggaccggccg gcggacggga gcgccgacct
    15121 gcgcggcctg ccgtgctgcc ccggcgtcgt cgagggcgtg gtcaaggtcg tgcaggcgcc
    15181 gggcgacgac ttgaagctca acggcgagat ccttgtcgcg ccgcggacgg accccggctg
    15241 ggtgccgttg taccccagcg tgtcgggcct gctcgtcgag cgcggcagcc tgctgtcgca
    15301 ctccgcgatc gtcgcgcggg agatgggcct gccggcgatc gtcgcggtcc gcggcctgac
    15361 cgcgacgctc tcgtcgggca tgcgcgtacg gatggacggc caggcgggga ccataaagat
    15421 tttagaccgc taattaacgc gaattgacgc gaattcggaa atgactttcc ttcgcgttaa
    15481 ttggcgttaa ttcgcggtga agagaaagga acggaaccat gcccgcgcca aggaagattg
    15541 cggctgaaat cctgaaccat cttctcgccg gaacgaaggc cggggacgag gtccatcggc
    15601 tcgctcgccg gccgccggaa aaggagccgc tgccgcccca cgtgccgcat gggaacgacc
    15661 atacggcggc ggggcttgaa aaacgtcggg ccttcctcaa ggcgcagggc ttttgcatcg
    15721 agcaactctc gggcctgggc ccggaccccg cgccggaatc gctggagggg cacatcgaga
    15781 atttcgtcgg gtacgcgaag gtcccggtgg gcgtcatcgg cccgctgcgg gtcaacgggg
    15841 cgcacgcgca cggcgatttt tacgtgccgc tcgcgacgtc cgagggcgcg ctggtcgcgt
    15901 cgtacaaccg cggcgcgtac gtcatcagcc agtccggcgg cgcggcggcg atgtgcctga
    15961 ccgagagcgt gtcgcgctcg ccctgcttcg cgttccgctc gctagccgac gcgggactct
    16021 tcctcgcgtg gacgctgccg cggttcgaat cgttccagaa agtggtggcg accacgtccg
    16081 gccacgcgcg cttgctggac atgcggaccg tgatgctagg caaggaactg tacctgatcc
    16141 tcgacttcac cacgggcgac gcgtccgggc agaacatggt cacgctcgcg gccgacgcgc
    16201 tggcgcgaca cctcgtcggg agcgcgccgg tgaagcctga acgctggtac gtcgagggca
    16261 acctgtccgg cgacaagaag gccacgatgc tgtccttcct gttcgcccgg ggcaagaagg
    16321 tcgtggccga ggcggtcgtc cggcgcgacc tgctgaagaa agtcatccac atcacggtcg
    16381 acgagatgct cgcgggctgg caggtctcca tcctcggcgg catccagagc ggcgcgatcg
    16441 gcgcgcaggg ccatttcgcc aacgcgctgg ccgccctgtt catcgcgtgc gggcaggacg
    16501 ccgcctgcgt cgcggaggcg tccgtgggcc tgacgcgcat ggacaagacc gcggacggcg
    16561 acctgtacgt gtccatcagc ctgccgaacc tgatcgtcgg caccgtcggc ggcgggacga
    16621 ccctgcccgg cgcgcgggaa ggcctggcga tgctgggctg cgcgggggaa ggcggggccc
    16681 ggaagttcgc cgaaatctgc gcggtcaccg cgctcgcggg cgagatctcg ctgaccggcg
    16741 cgatcatcgc gggcgagttc tcgaaggcgc acgcgcgcta tgggcggaaa cgcaaggcgg
    16801 agaaacccga tggcggatga cctggcgcaa cgcgtgcggt tcgatttcat ccactacgcc
    16861 aactgctggg aggatccccg gctgctgttc gaggcgctgc aaccggtcgg caagaggatt
    16921 ctctccatcg cgtccggcgg cgacaacagc ctcgcgctgc tcggagaggg cgcggaggtc
    16981 gtcgccgccg acttgagccg cgcacaactg gcctgcatcg agctgaaagt ggcggcgatc
    17041 cgggaactgg acgacgaggc tctgctcgcc tttctcggcc tccggccctc ggcggaccgg
    17101 cttgcggtcc atgacaaact acttcgcctg cgcctgtccg aaaacgccgc ggcgttctgg
    17161 aacgggcatc ccggggacat cgctgcgggc atcgtccacg ccggcaagtt tgagaactac
    17221 ttccggctct tccggacgcg cgtgctgccg ctgatccacg ggcggcggac ggtggagtcg
    17281 ttgctggcgg cccgcgacgc ggcgggccgc cgggatttct acgaccggcg ctggaacaac
    17341 cggcgctggc ggctgatgtt ccggctcttc ttcagccgat gggtcatggg gcgattcggc
    17401 cgcgacccgg aattcttccg ctacgtcgaa ggctccgtcg ccgaccgcat cctggcccgc
    17461 gccgagcacg ccctgaccgt tctgccgacg cacgacaatc cgtacctcga atacatcctc
    17521 acggggaatt tcgcccgcgc cctgccgccg tacctcgagc ccgaacgcct ggcccgtgtc
    17581 cgggccaacc tggatcggct cacgctgttc gagggcccgg cgcaggaggc gcccggcgag
    17641 ttcgacggct tcaacctctc cgacatcttc gagtacatgc cgcccgatgt agccgagcgc
    17701 gtctacgggc aactgctcga caaagcccgg cccggcgcgc ggctggccta ctggaacatg
    17761 ctcgtcccgc gcgcctgccc cgaaccgtat cgcgcgcgcg tgaaggcgct ggacgaactc
    17821 ggcgcccggt tgcttgagca ggaccgcgcg tttttctacc gtcgcttcgt ggtcgcggag
    17881 gcccgggcat gaaagcgcga ttaacaaaca aacgcaggtt ttcgctgacc cgccgtgacg
    17941 cggaactgtt caggctggcg cgccgggcgg accatcggac gctggcgatc tgggcgcggg
    18001 attgcgcgaa gcgagtgatg ccgtatttcg aatcggcgcg gcctgcggat ccccggccgc
    18061 gccaggccct cgaagcgctg caggcgtgga tcgataccgg gcagttcaag atggcggtca
    18121 tccgcaaggc gtcgctctcg tcgcacgcgg cggcccgcgc ggtcggcgag gacaatgccg
    18181 cgcgctccgc ggcccgcgcg gcgggccagg ccgtcgccac cgcccacgtt caaacccatg
    18241 ctccgggggc ggcgttgtat gcccagcagg cccgccaccg ggctgccgat cccgcggagg
    18301 ccgacgccgc ggcggccagt gaacgcgatt ggcaattggc gcatttgcgg aaacggatgg
    18361 ggaaggaata ggtggggagt ggaagagtct tcgtcatggt gggcgggcgg ggcggcggat
    18421 ttgtggggcg cgctgctgtt cggcgcgttg ttcctgcccc tgctgggcgt ggcggaatgg
    18481 ctgcgccggc gcggttcgtt cgggccggag gcgacgcgga aactggtgca catcggcggc
    18541 gggctgttga gcctggccct gccggggctg gtccggtcgc cggccgtggt gctggccatg
    18601 agcgcggcgc tgtcgctgct gttcctgtgg gccaaacgcc gcgcggccct ggccagcctg
    18661 cacggcgtcg cccggcggac gagcggcacg gagtatttcc cgctggcggt gttcctcgtc
    18721 tacgtcgcca cgtacggcca gtactggctc tacgcctgct cgatgctggt cctcgccgtc
    18781 gcggacgcat tcgcggcgct gatcggatcg aggtacgggc ggcaccggta cgaggtggag
    18841 gacgaggtca agagcgttga ggggtcgggc gtcttcctgc tgatcgcgtt cctcgcgatc
    18901 ctcttgccga tgctgctgct gaccagcttg ccgcgcgtcg tgtgcgtgct gtcggcgctg
    18961 ctggtggcgg tgctggtgac cgggttcgaa gcggtgagca tgggcggggc ggacaacctg
    19021 ttcataccgc tcggcgtcgc cgtgatcctc gccaagatca cgacgaagcc cgtgccggag
    19081 atcgtgtatc agaatgccag cctggccgcg ttgagcctcg gactggcgct ggcggcgcgg
    19141 cggactccgt ccttcaacgc gggcggcatg atcctgttcg cgttgttcgt cttcggcgcg
    19201 tggtcgctcg gctcggagcg gtgggcgctc ccggtgctgg cggctttcgt ggtctacgcg
    19261 ggagcgcgaa cgttcatccc cgggcccggc ggggccccgg cgcgcgtccg cgtgcggata
    19321 ttgttccggg tcctggtgat cccgctgctc ctgctggtga ccggcaacat gctccggcgc
    19381 ggcccggagt tctacgcgcc gttcgtcacc gcgatggcgg tggtgctggc cttcgggcta
    19441 tggaaccacg tgctgtgggc gcggcgcccg gccggcgccc ggcgccgggc gctcctatgg
    19501 ctggccgcgc ttgccgcgtg ggccgtggaa atcgtgccgg cctggctgct gctgccgggg
    19561 cgtccggcca cgtccggcct gtggcttccg gcgggacttg tcctggccgc gtccgcgttg
    19621 gacgatctgt tgctcgggcc cgatccgtcg ttcgaggggg agcgcgcctg gccggcctgg
    19681 cggctcgttc tcgcggccgc ggcgggcgcc gccgtgctgc tcgcgcagcg cgcgggcggg
    19741 ttggcgccgt ggccgtatcc cccatgatgt ccgccgctcg tcgccgtagg aattgcccgc
    19801 aggatggcct tgtgctatgg atccaaaaag tgcggctccg gagaagctat gaaaaaatat
    19861 atgccgcgcg aactggccgg cctggctgcg aaaatccgcc gcttcaaccg ccaccgcgac
    19921 tgggacaagt accatgcgcc gaagaacctg gccatgggcg tgatgatcga ggcggcggag
    19981 ctggccgaac atttcctctg gcaggagacc gcggagagcc gacggcccga cgcgcgcgcc
    20041 cgggcggcga tccgcgacga ggtgggcgac gtgctcatcc tcctgctgaa cctctgcgac
    20101 aagctggaca tcgatcccgt cgcggccgcg ctgaacaagc tggagcacaa ccggcggaag
    20161 tacccggcgg ccctcgtccg gggcaaggcg ctcaagtacg accagtaccc ggagcgggcc
    20221 gcgcgcggga cgaagcggcg ccctcctcta tccgttcggt aagagtctac ttcaccaaca
    20281 acggggagat cggtgcagcc gagggcgccg ttcaggcgcc ggatttggtt gagtttgtcg
    20341 gcgcgtcagc atcggcgtcc acactcacga acgccatgct agcgggtaga gccgatggaa
    20401 cgtttttcac gataagcgcg gatgcagttg acatgacgac cgttttcaca ttccaagcat
    20461 tcagaatgac gggctccgcg caatttggga cggccggatt gatgacctac caccaggttg
    20521 tttatgttcc gaatatcagc ttctccgcga ggtacaatgg cagcattgag cctccgcgct
    20581 tcctctcgct ggtcgaattg ccggtgatcg actccatgca gcgcgatggg aagctgtcgt
    20641 tctccggagt cgccacgagc accgtggcgt ccgtcgagtg ggccgccagc ctggcggagc
    20701 cgggccgaac caacggggta tccgctcctt agcgttcccg tgacccatgc cgtgacgacg
    20761 aacgacctcc cgaggttttt cagggtccgg ggcatcccga caccggacga aggtgtcctg
    20821 ccgccggcgc cgccgtgctg ctcgcgcagc gtgtgggcgg gttggcgccg tggccctttc
    20881 cccacggatg cctccggctc aatcgttgcc ggattctgtg actctcggac cccggaagca
    20941 ggggatgccc agcgtatccc gaagcgcgga tcgatttggt tgtccggggc ggggaaggct
    21001 gggctccagt tgtcagggcc ggtccacggt cagccagtag agccgtcgcg ttgcgctttc
    21061 ggggggggcg ttggtgataa agacccgatc gtcctggatg agggacactt ccgcgagcgt
    21121 ctcggaaggg gccggcagat cggcgctccc ctgcagggta taggagcgcc ccgttcgtcc
    21181 ttgccaggac agcacgcatg acggcaggtc cgaaggcgca aaggcctcca gccgaaagaa
    21241 ggacattccg tttgtcgggt ccgtcccagc cgcttgctcc tcggcatcgg tgaaggaatc
    21301 ttcgtcagag tcttttccgg gcaggccgtt ggttgggctg ccaaagaaat gatattccca
    21361 gcggtcgtcc agcccgtcct cgtcgcggtc gagccggaga atcagaaaga cctcattcga
    21421 cacggcatcc tggtcatcca caaacagcaa ggccatgtgt tccatgcaca cgtccatgtt
    21481 ccaacccggc gatgtcgccc cgatctcgta taggtgatcc cgggcgccgt tggtcatgac
    21541 ctggaaccat gacggggtgt catccgcaaa agttcccagc agtccgtccg atgagaaatc
    21601 gacggccgaa acctgtctaa acccaaggga ggtttccgac caggggatcg aacaggggga
    21661 caggtaggcc gccgggccgg cggtaaacac caggcaagcc gacgcgccgg tagtcatgca
    21721 taagcaggag tcccggagga atacatgatg gtaatacgac cattcggatg acgcgatctg
    21781 ggtgaggggg tattcgatgc ggatgtactc gatccgctca tcatgcgttt gtggacagaa
    21841 gacgccgccc ggcttgtagg aggcatcgcg atcccgttga tggcgggccg ccacgtggat
    21901 gacgccgtcg gcgcaacgca gcaccgggtt cacgtaatcg tgatgttcgt tggctgcgcg
    21961 cccggcgcag gtgtacgtgg ataagctgcc gtccaatagc atgccgagat cgtacaccga
    22021 acctgtcgcc ataacggcaa ggaccacctc atgataagta tccccgtcat actcccgggc
    22081 ggaatacgcc aggtaggccg cttcatcgtt catgaagcac aacgacgccg catctcccgc
    22141 ccggatcggg gtgtttgtcc acaggccgcc ggtcctggcg gcgtgcacga ccacgccgga
    22201 gcaaacggtc aggacatgag gttctccggc gctggtcacc gccagggaag ttccggttcc
    22261 gcccgcgacg agttcttccg ggaccacgca agcgccctcg ccggtaaacg actgatagta
    22321 cgtctcgctg ttccgactgt agataatatg cagccggccc cgggggtcac actccatcag
    22381 gggggccgtc tcatcccatg cgttggtcgt aagttgaagc tgcctatagg aggcgacgtc
    22441 ccgaccttcg ccatcgcagg tcgcgacaca aagaaacaag ccgacgtata caggccatga
    22501 tgggtggcgc ctcatgctgg gaaccccggt ccgaagttag ccgcgcggca tggttgacgg
    22561 caacataaat cggccgtatg ggcgggggct cggacgggaa cgcggcccca tccggctcga
    22621 cgccgggccg cattggaatg accggtcctc ggcatggagg actcgtcgcc gccggccgag
    22681 gcgggcgtca gggcgccagg gccttgcgga cgatttcttc gacggtggtt ttgggcccga
    22741 cgccgggcag gacgtcgcgg acgcgtttct gggcgtcggc ctgcttgtag ccgagggaga
    22801 ccagggccag gatggcgtcg cgcagccgcg cgtccggctc gccggcggcc gccccggcga
    22861 cggcttcgag ggcctcgccc gcggtgagct tgtcgcgcag ttccacgacc atgcgctcgg
    22921 cgg
//
LOCUS       DAOOTK010000042        22425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1585_length_22425_cov_4.836567, whole genome shotgun sequence.
ACCESSION   DAOOTK010000042 DAOOTK010000000
VERSION     DAOOTK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22425
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1585_length_22425_cov_4.836567"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PK634_06680"
     CDS             complement(<1..698)
                     /locus_tag="PK634_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOW97459.1"
                     /translation="MKPTRRLYALVGAVLTLLMFRHPAEAQRARIESRAADPYQGAIL
                     LAADGEVLFEDKADAPGYPASMVKMMNLLLILEGIDRGAWKLQDPIVITAESAGMGGS
                     QVFLKEGEMFPIEELLYAMMIQSANDAAVALALKVAGTREGFVELMNRRAAELGMRST
                     RFHSVHGLPPAEGQEPDVSTPRDMARLCLALLRRPDALPFTSTIKRPFRPDAPEPFIM
                     ETHNRLLKTFEGCDG"
     gene            complement(695..1747)
                     /locus_tag="PK634_06685"
     CDS             complement(695..1747)
                     /locus_tag="PK634_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOW97460.1"
                     /translation="MSLGEIIGPVQRWDAAGRSTCIALLFRVEGSSPRPPGSRLAVND
                     QGEMAGYISLGCVEGDVREHVRQVLGGAPASVVHYGVADETALGVGLSCGGRIDVLLT
                     RHEPADPAWRALCAASARGHAVLVTRIQPADGRQMLLDSAGTQWGGLGMASLDQQAAQ
                     AARDLEGERRAILRPLGEDLCLFEPISPPARLILVGASPVAASLCKLASAIGMDVTVV
                     DPRRDFANPQLFPGARRIVLEWPEKGLAEAGAGPEDGVAILSHDAKFDLPGLESALRA
                     GCRYVGLLGGRKTQEQRRKALAERGFSADDLARLRGPIGLRIGSVTPDEIAVSILAEI
                     ISLSPSAIRKKEEHHE"
     gene            1854..3299
                     /locus_tag="PK634_06690"
     CDS             1854..3299
                     /locus_tag="PK634_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOW97461.1"
                     /translation="MSHAMNDRDPVLTPGGYRETLKVAWPLILSMGSFTLMQFTDRLF
                     LAWYSAVSIQAALPAGILSFTMVSGFMALAGYANTFVAQYHGAGNGPGCSRATAQGTL
                     LALASWPLMLALIPLGLWLLRISGHAPAVLAEERAYFAILMLGGVTAPLGAAVGGFFS
                     GRGDTFTTMVTNVMGNVLNIVLDYAMIFGRWGFPEMGIRGAAWATVISGFVSPALLLL
                     LYFSRRYDQPFRTRAEFRWDAVLLRRMLRFGLPAALHLVLDVGAFTLFVLLTGRLGAT
                     ALAASNIALSINNIAFMPLIGISIAASILVGQYQGRRQGAIAEKAGWTSLKLGWIYMG
                     VIALTFALFPQAYFRLFTWRGGAELPLENILPVGRWLLLMMAAWGLLDAVNLILSGAL
                     KGAGDTRFVMIYSVVMAWGFWMSGEMVIIFLLGGGIVPAWAWMTAFVFVMAAGFLWRF
                     RSGRWKTIELIERQTPIQPTRPGAEALIAGD"
     gene            complement(3390..4109)
                     /locus_tag="PK634_06695"
     CDS             complement(3390..4109)
                     /locus_tag="PK634_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97462.1"
                     /translation="MDTGPAIPPRAAERETGSPRLEAAELLLLARGLLCVFWGIPLSL
                     LMFFGTLELRLAAGLRVPVFAFGLMLIFLGVVHMSRVKSLSPRWARRTRETQLILLIL
                     VYLAPFVYWWQAMPHVPYYCVNVLAIVVVSMWGFLAVNRLAAEIGNRFGEHAFVIEAR
                     LSGWLAVVFLLGPGTFAVLRTILATASIDNSGLATLVLVPYLVPRWMYALTLLPFTMT
                     MMMVWRAKELCLRAIRQAEPV"
     gene            complement(4162..4632)
                     /locus_tag="PK634_06700"
     CDS             complement(4162..4632)
                     /locus_tag="PK634_06700"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOW97463.1"
                     /translation="MANVKKVTVTAHQTESMRMDLQARHHRVIIDQPASSNGQDAGPT
                     PLEYLVASLAGCMGAIGRIVARQQKLNIRWMDFVVEGDIDVDGLLGRSTTSRVGFQGF
                     RIKARIDGDLSPEQKQAFLHEVERRCPVSENLVNATPIRLEVVEDLGGIKGPAV"
     gene            complement(4691..>5501)
                     /locus_tag="PK634_06705"
     CDS             complement(4691..>5501)
                     /locus_tag="PK634_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97464.1"
                     /translation="DHFIFVVTNPDAWVSAPWAKSGTVPPWSAYLADENDSLYASWFN
                     AGGSAITDLGLARCAAYFENGGRIEGVIDLAAILGSGFTQAVYLAVAPYSSSDGGALQ
                     SAYQVPAGNGNGDLWGSSEFFQLAPGDADGDGINDDADPDADGDGMADAWEEFRFGDL
                     DAADGGDEDGDGATNLEEWRACTDPGDTGSVFRIQYADASTTGLALSWPAAYGKTTAV
                     YRAEGPCFSNHLAWTSAQVFAGPTNFPFGTNRYSAGFDSSARFFRLGLSSR"
     assembly_gap    5502..5511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5512..6796)
                     /locus_tag="PK634_06710"
     CDS             complement(<5512..6796)
                     /locus_tag="PK634_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOW97465.1"
                     /translation="MLAGLMLAAMSLSARADEVRVMSFNLRYASAAEDQNSWKQWDST
                     NHLPQRRQFVCRVITNRAPDVIGFQEGEDEQLDYLEANLPAHYGIERQKPSGGSGNEN
                     AAFGYNTNTVELLDRGVFSLGPSPGGGYWNNPAGTNFQPYLYFTNMGWSFPRLALWGK
                     FRWRATGQEFLFYTTHFDINNDPQVASAKLIADDALCRAARMPGSPLAIVVGDFNSYH
                     ADNDWKLFTGSHTFDGVTGDFTDAWQQVHGTWTGSGTFHGWAGGTPSTSQHIDWILHR
                     GGFTATQAVVVTDTTTATNLSDSSTSTLYPSDHYPAFAILRLPAVASDYDGDGLPDAT
                     ERALGDINPVDADSDGDGLLDGQEDLDGDGTVDGGETDPSTANAGAQRPTDVRNAQMD
                     GILDHDAAFAAENSGMNLYYKFDGRYLYVATWDARE"
     gene            6899..8212
                     /locus_tag="PK634_06715"
     CDS             6899..8212
                     /locus_tag="PK634_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW97466.1"
                     /translation="MGRYLARRLLQMIPTVLGVLLITFVLFNVVGGSPASMVLGEHVS
                     PATLEDFDEQRGFNKPLLFGRWAKTRAWPDGRRPDPAAWEPGERIVPLKFALRPNTGY
                     RLVLEARAAGGDFPNIGKTKVHFFQCLEKTGAFFPMSGKNDAEFSNVWKKTEIIFQTL
                     ESTSASDLRLAVQGTPLEIRSVQLRRRMASPWDSQFFHFLRKIARLDFGTSSSLNQPV
                     TRLLAEGIGPTLALTIPIFTLELALAVSLALVCAFWRDRWPDRLAVVVAVGLMSVNYL
                     VWIVAGQYVLAHRLGWFPVWGFESWRYLLLPVVIGVISGLGSSLRFYRTVMLDEMGKG
                     YVRTAFAKGVPRRGVLFRHVLRNALIPLITNVAVTLPYLYTGSLLLESFFGIPGLGYL
                     GVNAVNSADVDVVRAIVLIGSALYVIVNLLTDVAYAVVDPRVRLE"
     gene            8209..9102
                     /locus_tag="PK634_06720"
     CDS             8209..9102
                     /locus_tag="PK634_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW97467.1"
                     /translation="MKEGSGGRSLWAEAWTRLRGRRMTRACLWVIAAYTLMAVYGEVV
                     YRVNHALDRTPSYQRTDLSQAYRPPSWRHPMGTDGLGRDVMQRLVQGTRVAFQVGIVT
                     SLIAIPIGVVLGLLAGYFGGRTDDLIVWLYSTFASVPGLLFILAIAMVVGRGLLGVYL
                     GIGLTTWVGICRLIRGETIKHRAQPYVLAARALGLGNARILFRHILPNVFHIVIVTFT
                     LRFPLSIGTEVFMSFLGIGVQGEPSWGTMIGNARLRLWHGAWWEMTFVTLAVFGLVLA
                     FNLLGDALRDALDPRLRTAER"
     gene            9190..12789
                     /locus_tag="PK634_06725"
     CDS             9190..12789
                     /locus_tag="PK634_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97468.1"
                     /translation="MRNLVVAVAVCVGFASALAQVPSLINYQGRLVNDTNLVNETVGL
                     SLQLYDDPSAGTLLYEDSNQVAVVDGLYSTFIGDDTISGTLTGALASAEVWIQVLING
                     APLMPREQLVSAGYALQAADVPGNVFWRTDGNAGTTAGTHFVGTTDEQPLHLRADNAT
                     VLRLDGEPNPPNLIGGHASNYADPGVNGAFIGGGGDPLGFSPNRVSDHYGVVGGGMNN
                     VAGDTGDTVSNSSFATVAGGSANTAYGAYSFIGGGAINQIFGASPYSVIAGGYNNIAR
                     GTNATVGGGSLNEARRAGATIAGGENNYAEGPYAFIGGGRQNKANDSYSFSAGHRAES
                     LHEGSFVWADNSTPTAFASTSSNQFLIRAAGGVGINTNGPFTEALTVNGGIRATGSVA
                     AASFAGDGSGLTMLHGTNLVIGSVSNAAIGADAVTGDKVMDGTITDDDVAANTFWQTT
                     GNGGTAAGTHFVGTTDTQPLDLRANNTRVMRLDGNYNPPNVIGGSMANTVDVGVAGAT
                     IGGGGDPAMVFINRISDDYGFVGGGLGNRAGNSGGTAADAAFATVAGGLKNVANGLFS
                     TIGGGDMNAVTTVAQGGAIAGGETNVVSGRNAFIGGGKLNAATGSWSVVAGGLANRAE
                     GRGSAVGGGSNNVINSQAWDSIIAGGFNNRADENRAFIGGGQGNYSTGQMSVIAGGYM
                     NTNMAVGAVIGGGGLNFNTGVYATVAGGLFNKALGPNAVIGGGLRNEAAGYAAVVAGG
                     TNNSTLSGMTAIGGGGLNFIGVNAEYATIAGGQSNTVNDTSPGAVVGGGRNNVAGGSD
                     VMVGGGQANLAAGAFSTIAGGSYNTANGMGSFIGGGTNNTTAPWAAYSVIAGGAANVM
                     DTHESFMGGGSTNTIRTNAYQSAIVGGNGNTIETNAYRSVIVGGYHNTILAYADHNAI
                     VGGMANVLGGSYFGFIGGGNENEIQGDYDVLVGGNDNYISSQANYAFIGGGSGNIITN
                     FYGHGSFIGGGYYNSVSGRWSFVAGGYRNLMGGEYGVIAGGSSNVVNGDYAVVPGGRE
                     NIASAPYSFAAGRMASAVHTGAFVWADSTGSTFSSTTNDQFAIQASNGVYIAQDLGSD
                     RKAKFGERFRDNSVVAWAKVDDDGSLLASFNVHTVTNAAVGRYDILLKSGARLSSACI
                     PVATAERDGQPASAATVRLVSIDQTATNHFRVYINDGTFAPVNNEFTVAVFGR"
     gene            12878..13582
                     /locus_tag="PK634_06730"
     CDS             12878..13582
                     /locus_tag="PK634_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOW97469.1"
                     /translation="MSTTIERLLIIQERDRAIARLVRETKDIPARKKLIEDRLKAHQD
                     VLHTAQEDLKKAQAGIKQVEIEVDSRRQKIQRFREQQIQIKKNEEFRALEREIAAVQK
                     EIRAEEDREIEFMEQVEKARAVIAEREADLKKEDARVREDQKVFDRRLEELQAEIAKL
                     QTDRKALAEQVDATWLGRYERILAKTGDYALVPVEHGACGGCHMNLPPHLIHEARKGL
                     SAAQCSYCSRMLYWKP"
     gene            13688..14084
                     /gene="rnpB"
                     /locus_tag="PK634_06735"
     ncRNA           13688..14084
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK634_06735"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            14160..15038
                     /locus_tag="PK634_06740"
     CDS             14160..15038
                     /locus_tag="PK634_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97470.1"
                     /translation="MRGLSIARWVALLVAVAAVAGFALLYRRLPSSPPAAAVPEAVLA
                     WPSPSAPDAEDWAVFQPAAGAAPAELSPLARRFRLAGTFFSIGEEQDERPPSARLAIL
                     DDVEKKQQFVVREQETFDDVQVVRILQDHVVLTSHGQQEELWLSFHGEAAESPPPAAP
                     AGEAVEPTLEQNRFGRRVGETRWVLSRQALMDYYQELANDPERIAALYMTMKPDYKEN
                     RIAGYQVDIEGEQEFLDAVGLKQSDFIRKVNSMNMTSQRRAEYFISEFLQERVSAVVL
                     DIEREGKEQKLIYLIR"
     gene            complement(15023..17203)
                     /locus_tag="PK634_06745"
     CDS             complement(15023..17203)
                     /locus_tag="PK634_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOW97471.1"
                     /translation="MAEFTPSERKRILDFLHRPDYRPLRQHELARALAWSGPVLRRAL
                     RDMEREGLIVRLRKNRWAAPRGAPTLAGRLRVSVDGFGFVTPEQTGRPDIYIPAESLG
                     PALDGDRVLIALSTEGEVPDEREARTRGRVVRVLERRHERVVGRLRRTAYYGYVIPDN
                     PRIRENVRVRGFAPGLAQPEEDRLVVVDLEPREREQESLSGTVVEDLGPADAPGADML
                     SVMRSFDVRSEFPAEALRQADHESPAPAPALFHDRHDLRGQLVFTIDPEDAKDFDDAV
                     SLVRADDGRWRLGVHIADVSRYVPVDSPVDVEARRRGTSVYLADRVIPMLPPRLTTDV
                     CSLQPGVPRLTHSVFILFDPLGGVLDFSTGPSVIRSAARLDYDQVQACFDGGAGGSIP
                     AEVRASLAEMRELAALLRRRRMAEGSLDLAMPEIRCRMDSAGRVLEIRKRGSTEAYQL
                     IEEFMLAANQAVAGRIARQKAPSLYRIHDEPDAAQWERMQVELRALGLASLPPRKDTL
                     SRLARLAAASPMSYAAQLAILRNLKRAVYAPVPGLHFGLGFDCYTHFTSPIRRYPDLV
                     VHRVLKALETRAAPPYSADDTARLATHCSQTEAQADEAESDSIVRKRLDYYHGLMARD
                     ETGPFEGVIIGLHARGLVVELRDTLQRGHLPFSALPEDHYVTDRDRMKITGRRRDRSW
                     RVGQEVRVRLARVDARRRSVGLLLEEASADARTRRREKRRQRMR"
     gene            17272..18714
                     /gene="glgA"
                     /locus_tag="PK634_06750"
     CDS             17272..18714
                     /gene="glgA"
                     /locus_tag="PK634_06750"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOW97472.1"
                     /translation="MRVLYAASEIMPFASTGGLADVGAGLPKALLARGGDISRIMPLY
                     RRVAEGGYALKDTGLRLDIPVGFQMHQAEVWYTEDPPPRTYFIRRDEFFDRSQLYSLP
                     ERDYDDNFERFVFFQKAIVSLIDTLQWHPDIVHCSDWQTGLLPLFLRHGIQGSGRKQT
                     ERTIFTIHNLAYQGLFPGSQYSQTNLPFFCFNMDCAEFYGSINCIKAGITSANLVTTV
                     SQTYAQEIRTEEYGCGLHGILAQMGDRLVGVVNGVDYSLWNPKVDPHLAKAYGPDDIS
                     GKKDCKEELLRVMGLTISPEAPLIGMVSRLVDQKGLDILTEAMPALMESNAGFVLLGA
                     GQDKYQKLCLEWASRWPGRFAARLGFDNPLSHKIEAGADIYMMPSRFEPCGLNQLYSL
                     KYGTIPVVHATGGLDDTIQDIDTEGHEGTGFKFRTYTAEGLAHATQRAIALYQRSDLW
                     PSVIRRAMAQDFSWERAAEDYQKRYQQLMS"
     gene            complement(19018..20112)
                     /gene="lepB"
                     /locus_tag="PK634_06755"
     CDS             complement(19018..20112)
                     /gene="lepB"
                     /locus_tag="PK634_06755"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOW97473.1"
                     /translation="MNLFQFRRYRQWVHHLVQESRHVVNVRGDILPAEALEELARRTQ
                     ALQEAWKQRHPEHVEEAAEELSEYILRINPPRRFAKITEYVEIITVALAVAMGFRTYF
                     IQPFKIPTGSMQPTLYGITVGTQYAPKWHDRFPVSLVSMALFGERYTEVKAKVSGQVG
                     SQFGNEEEHIVFYVQDVPHPVRKGMTLFFAPGDYVAKGQVLASGRVKLGDHIFVNKLR
                     YNFSRPRRGDITVFSTDGIQYPNIRPNSFYIKRMTGLPEETVAIDPPYLVVDGKKITE
                     PYPFARLVNERDKGYVGYSLARDQAAIPVYLGVQGESRVLGDDEYLMMGDNTEHSYDG
                     RYFGPVKKEYLVGPAFMVYWPFTRRWGRVQ"
     gene            complement(20123..21925)
                     /gene="lepA"
                     /locus_tag="PK634_06760"
     CDS             complement(20123..21925)
                     /gene="lepA"
                     /locus_tag="PK634_06760"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOW97474.1"
                     /translation="MSELALIRNFCIIAHIDHGKSTLADRMLELTGTIQKREFRDQVL
                     DDMDLERERGITIKAHPVTMNYTARDGQTYCFNLIDTPGHVDFSYEVSRSLSACEGAL
                     LVVDVTQGVEAQTVAHTLLAAEHGIQIIPVLNKIDMPNADVVAVAHQIEEVLAIPMGD
                     SLQVSAKTGQGVAEVLEAIVQRIPSPVEKDEAHTRALIFDSEYDTFRGGVIYVRMFDG
                     TLSPGNRIRLMSIARDYEVKEVGIFTPKPVVRPTLEPGEVGYLMANIKNASDIQIGDT
                     ITRSDRPAPQPLPGFKEFHPMVFTGLYPVQSDDYEKLRASIEKFSLNDSAFVHQAETS
                     AALGFGFRCGFLGLLHMEVVQERLRREYDVDIIATYPSVIYRVYLTDGAMVEVDNPAH
                     LPELTKIERIEEPMIKAFIICHNEHIGDMMQLIMDRRGEVTKTESLDTRRVMLTCSVP
                     LNEILIDFYDRLKSASRGYASMDYEHAGYRESDLVKMDILVHGEVVEAFAAIVHRSKA
                     EYRGRQICKSLKELIPPHMFAIAIQAAINKKIIARETVRAFRKDVTAKCYGGDVTRKR
                     KLLERQKEGKKKLKQIGQVSIPQEAFISVLKTSD"
     gene            complement(21929..>22425)
                     /locus_tag="PK634_06765"
     CDS             complement(21929..>22425)
                     /locus_tag="PK634_06765"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOW97475.1"
                     /translation="TKLPLSVHLMMSRPDGLADAFLDAGANTLLIHVEARCDPAALLG
                     RIRERGARAGLTLNPETPAEAALPYRERADEILCMTVHPGFGGQSFLEEVLPKIRGLR
                     EALPGMALSVDGGLNDETTARCAEAGADIFLVGSSLFTADGDMAARIRSMRAAIQKSL
                     AGRG"
BASE COUNT         3794 a         7321 c         7486 g         3814 t
ORIGIN      
        1 ccgtcacacc cctcgaaggt tttcagcagc cggttgtgcg tttccatgat gaacggttcc
       61 ggcgcgtcgg gacggaaggg ccgcttgatc gtggaggtga acggcagcgc gtccggcctt
      121 cgcaacagcg ccaggcacag ccgggccatg tcgcggggcg tgctgacgtc cggctcctgg
      181 ccttccgcgg gaggcaggcc atgaacggag tggaagcgcg tgctgcgcat ccccagttcg
      241 gcggcgcgcc ggttcatcag ctccacaaag ccctcccttg tcccggccac cttgagggcc
      301 agggccacgg cggcatcgtt ggcggattga atcatcatgg catacagcag ttcctcgatc
      361 gggaacatct cgccttcctt gagaaacacc tgcgatccgc ccatccccgc cgactcggcg
      421 gtgatgacaa tgggatcctg caatttccag gcgccgcggt cgatgccctc gaggatcaac
      481 aggaggttca tcatcttcac catgctggcc gggtagccgg gcgcgtccgc cttgtcctcg
      541 aacaggacct ccccgtcggc cgccaggagg atcgcgccct ggtacgggtc cgcggcccgg
      601 ctctcgatcc gggcccgctg cgcctccgcg gggtgccgga acatgagcag ggtgagaacg
      661 gcgccgacaa gcgcatacag gcgacgggtg ggcttcattc gtgatgctcc tctttcttgc
      721 ggatggcgga cggcgacagg gaaatgattt cggccaggat ggagacggct atttcatccg
      781 gggtgaccga tccgatgcgc agcccgatcg gcccccgcag ccgggccagg tcgtccgcgg
      841 aaaacccccg ctcggcgagg gccttccttc gctgctcctg ggtcttgcgc ccgcccagca
      901 acccgacata gcggcatccc gcgcgcagcg cgctctccag gcccggcaga tcgaacttgg
      961 catcatggct caatatggcc acgccatcct cgggcccggc ccccgcctcg gcgagccctt
     1021 tctccggcca ttccagcacg atccggcggg cgcccggaaa cagctggggg ttggcgaaat
     1081 cgcggcgcgg gtcaacgacc gtgacgtcca tcccgatggc cgaggccagc ttgcagaggg
     1141 aggcggcgac gggcgaggcc ccgaccagga tcagtcgggc cggcggagag atcggctcga
     1201 agaggcaaag gtcttcgccc aggggccgca ggatggcccg gcgctccccc tccaggtccc
     1261 gcgccgcttg cgcggcctgc tgatcgaggg aggccatgcc cagtccgccc cattgcgtcc
     1321 ccgcgctgtc gaggagcatc tgccgtccat ccgccggctg gatacgcgtg accaggacgg
     1381 cgtgtccgcg cgctgatgcc gcgcaaagcg cccgccatgc ggggtccgcg ggttcgtgcc
     1441 gggtcagcag cacatcgatc cggcccccgc aggagaggcc gacgcccagc gcggtttcat
     1501 ccgcgacccc gtagtggacc acgctcgccg gcgcgccgcc cagcacctgc cgcacatgct
     1561 cgcggacatc gccctcgacg cagcccagcg agatatagcc ggccatttcg ccctggtcgt
     1621 tgacagccag gcgggagccg ggcgggcggg gcgacgagcc ctccacccgg aaaagcaggg
     1681 cgatgcaggt cgaccggccc gcggcgtccc accgctgcac cggcccgatg atttccccca
     1741 ggctcatgtt ttggtccggg cctgctttac cataaacgac ccggcgcggg aagccggtct
     1801 tttgcgcggg gaaggattgc ggaagcccgg acaaatccgg tagtgtgctc ggcttgtccc
     1861 atgcgatgaa cgaccgcgat ccagtcctga cgccgggcgg ttaccgggaa accctgaagg
     1921 tggcctggcc cctgattctc agcatgggct cgttcacgct gatgcagttc acggatcggt
     1981 tgttcctggc ctggtacagc gcggtctcca tccaggcggc gttgccggcg ggcatcctgt
     2041 cgttcaccat ggtcagcggg ttcatggcgc tcgccggcta cgccaacacc ttcgtggcgc
     2101 agtatcacgg ggccgggaac gggcccggct gcagccgcgc cacggcgcag ggtaccctgc
     2161 tggccctggc gtcgtggcca ttgatgctgg cgctgatccc gctgggcttg tggctgctgc
     2221 ggatcagcgg ccatgcgccg gcggtgttgg cggaggagcg ggcctatttt gccatcctga
     2281 tgctgggcgg cgtgacggcg cccctcggcg cggcggtcgg cgggtttttc agcgggcgcg
     2341 gcgacacgtt cacgaccatg gtcacgaacg tgatggggaa cgtgctcaac atcgtcctgg
     2401 actacgccat gattttcggc cgctggggct ttcccgaaat ggggatacgc ggcgcggcct
     2461 gggcgacggt catctcgggg ttcgtcagcc ccgcgctgct cctgctgctg tacttttccc
     2521 gtcgctacga ccaaccgttc cggacccgcg cggaattccg ctgggacgcg gtcctcctgc
     2581 ggcggatgct gcgcttcggc ctgcccgccg ccctgcacct ggtgctagac gtcggggctt
     2641 tcacgctctt cgtcctgctg acgggccgcc tcggcgcgac ggccctcgcc gccagcaaca
     2701 tcgcgttgag catcaataac atcgcgttca tgccgctgat cggcatcagc atcgccgcgt
     2761 cgatcctcgt cggccagtac caggggcggc ggcagggcgc catcgccgaa aaggccggat
     2821 ggacctcgtt gaagctgggc tggatctaca tgggcgtcat agccctgacc ttcgcgctgt
     2881 tcccgcaggc ctacttccgg cttttcacct ggcggggcgg ggcggaactg cccctcgaga
     2941 acatcctgcc ggtggggcgc tggctcctgc tgatgatggc cgcgtggggc ctgctggacg
     3001 cggtcaacct gatcctctcg ggggcgttga agggggccgg ggacacgcgc ttcgtgatga
     3061 tctactccgt ggtcatggcg tggggcttct ggatgtcggg ggagatggtg atcatcttcc
     3121 tgctgggcgg cggcatcgtg ccggcctggg cctggatgac ggcgtttgtc ttcgtgatgg
     3181 ccgccgggtt cctgtggcga tttcggtccg gccgctggaa gaccatcgag ttgatcgagc
     3241 gccagacccc gatccagccc acccgtcccg gcgccgaggc cctgatcgcc ggcgactgat
     3301 gcccgccggc cgatttccac actttgcaaa gtgtggaaaa tttcccgcgg tgaaaacgga
     3361 ccggattgcg ctcgcgccgc cgctggcctt taaaccggct ccgcctggcg gatcgcgcgc
     3421 aggcagagtt ccttggcccg ccagaccatc atcatcgtca tcgtgaaggg cagcagggtc
     3481 agcgcgtaca tccagcgcgg gaccaggtag ggcaccagca ccagggtggc gagcccgctg
     3541 ttgtcgatcg acgccgtggc caggatggtg cggagcacgg cgaacgtccc cggccccagc
     3601 aggaaaacca ccgccagcca gccgctcaag cgggcctcga tcacaaaggc gtgttccccg
     3661 aaccggttgc cgatctccgc cgccagacgg ttcacggcca ggaaccccca catcgacacg
     3721 accacgatcg ccagcacgtt gacgcagtag tacggcacgt gcggcatggc ctgccaccag
     3781 tagacaaagg gcgcgaggta gaccaggatc agcaggatga gctgggtttc gcgcgtgcgc
     3841 cgggcccagc gcggcgacag tgatttcacg cggctcatgt ggactacgcc gagaaaaatc
     3901 agcatcaggc cgaacgcgaa gaccggcacc cgcagcccgg cggccagccg caattccagc
     3961 gtcccgaaga acatcagcag ggacagcggg atcccccaga atacgcagag gaggccgcgg
     4021 gccaaaagga gcagttcggc cgcctccagc cgcggcgacc ccgtctctct ttccgccgcg
     4081 cggggcggga tggcagggcc cgtgtccatg gtcggattat gcgaaggccc ctgccgcccg
     4141 gcaagcgcgg ggcgcggaag atcagacggc gggacccttg atgcccccga gatcctcgac
     4201 gacctcgagt cggatcggcg tcgcgttcac caggttctcg gacaccggac agcgtcgttc
     4261 cacctcgtga aggaacgcct gcttctgctc cggcgacagg tcgccgtcga tccgggcctt
     4321 gatccgaaag ccctggaaac cgacacgact cgtcgtcgac cggccgagca gcccgtcgac
     4381 gtcgatgtcg ccctcgacca cgaaatccat ccagcggatg ttcagcttct gctggcgcgc
     4441 gacgatgcgc ccgatggctc ccatgcaacc ggcgagggag gccaccaggt attccagcgg
     4501 cgtcggtccc gcgtcctgcc cgttgctgct ggcaggctga tcgatgatga cccggtgatg
     4561 ccgggcctgc aggtccatcc gcatgctctc ggtctggtgg gcggtaacgg tgactttctt
     4621 gacgttggcc atggcgcgct ccttttgccg tcttgcctta atccagcagc gccgttttgt
     4681 caaaccgcgc ctagcggctc gataaaccca ggcggaagaa ccgggccgac gaatcgaagc
     4741 ccgcggaata ccggttagtg ccgaaaggaa agttcgtggg ccccgcaaaa acctgcgcgg
     4801 acgtccaggc caggtgattt gaaaaacagg ggccttccgc gcggtaaacc gcggtcgtct
     4861 tgccgtaggc cgccggccag ctcaacgcga ggcccgtcgt cgacgcatcg gcgtattgga
     4921 tgcggaacac cgagccggtg tcgcccggat ccgtgcaggc tcgccactcc tccaggttgg
     4981 tggcgccgtc cccgtcctcg tcgccgccgt cggccgcgtc gagatcgccg aatcggaatt
     5041 cctcccacgc atcggccatg ccgtcgccgt ccgcgtccgg gtcggcatca tcgttgatcc
     5101 cgtcgccgtc cgcatcgccg ggcgcgagtt ggaaaaattc ggacgagccc cagaggtcgc
     5161 cgtttccgtt gcccgccggg acctggtagg ccgactgcag cgcgccgccg tcggagctgg
     5221 agtagggggc cacggcgagg tacacggcct gtgtgaagcc cgagccgagg atcgcggcca
     5281 ggtcgatcac gccctcgatc cggccgccgt tctcgaagta cgccgcgcag cgggccagcc
     5341 ccaggtccgt gatcgccgag ccgccggcgt tgaaccagct cgcgtacaaa ctgtcgttct
     5401 cgtccgccag gtacgcgctc cacggcggca ccgtgcccga cttggcccac ggcgcggaca
     5461 cccacgcgtc gggattggtc accacgaaga tgaaatggtc cnnnnnnnnn ncctcgcgcg
     5521 cgtcccacgt cgccacgtac aggtagcgcc cgtcgaattt gtaatacagg ttcatcccgc
     5581 tgttctcggc ggcgaacgcg gcgtcatggt ccaggatgcc gtccatctgc gcgttccgca
     5641 cgtcggtggg ccgttgcgcc ccggcgttgg ccgtcgaggg gtccgtctcg ccgccgtcca
     5701 ccgtcccgtc gccgtcgagg tcctcctgcc cgtcgagcag cccgtcgccg tccgagtcgg
     5761 cgtccacggg attgatgtcg ccgagcgcgc gctccgtcgc gtccggcagg ccgtccccgt
     5821 cgtagtccga cgcgacggca ggcaggcgca ggatggcgaa ggccgggtaa tggtcggacg
     5881 ggtacagggt cgaggtcgag ctgtcggaaa gattggtcgc cgtggtcgta tccgtcacca
     5941 cgaccgcctg cgtcgccgtg aagccgccgc ggtgcaggat ccagtcgata tgctgcgacg
     6001 tggacggtgt gccgccggcc cagccgtgaa acgtgcccga gcctgtccac gtcccgtgga
     6061 cctgctgcca cgcatccgtg aagtcgcccg tgacgccgtc gaaggtgtgc gagcccgtga
     6121 acagcttcca gtcgttgtcc gcgtggtacg agttgaaatc gccgacgacg atcgccagcg
     6181 gcgagccggg catccgcgcg gcccggcaca gggcgtcgtc cgcgatcagc ttcgcgctgg
     6241 cgacctgcgg atcgttgttg atgtcgaaat gcgtcgtgta gaagaggaat tcctggcccg
     6301 tcgcgcgcca ccggaacttc ccccacagcg ccagccgcgg aaagctccag cccatgttcg
     6361 tgaagtagag gtacggctgg aaattcgtgc ccgccgggtt gttccagtag ccgccgccgg
     6421 ggctgggccc gagggagaac acgccgcggt cgagcagttc caccgtgttc gtgttgtacc
     6481 cgaatgccgc gttctcgttg ccgctccccc cgctgggttt ctgccgttcg atcccgtagt
     6541 gggcgggcag gttggcctcg aggtagtcga gctgctcatc ctcgccctcc tggaacccga
     6601 tcacgtccgg cgcgcggttg gtgatcaccc ggcagacgaa ctgccggcgc tggggcaggt
     6661 ggttggtgct gtcccactgc ttccaggaat tctggtcctc cgccgccgag gcatagcgga
     6721 ggttgaagct catcacccgg acctcgtcag cgcgcgcgga gaggctcatg gcggccagca
     6781 taaggccggc cagcaaggga agtcgactcc tcatgggcgt catcataccg cggcgaaccg
     6841 tttcaggtta atagcgcatt ctggacgacc ggcccgcggt tcgctaccgt ggcgcgacat
     6901 ggggcgctat ctcgccaggc ggctgctgca gatgatcccg accgtgctcg gggtgctgct
     6961 gatcaccttc gtcctcttca acgtcgtcgg cggcagcccg gcctccatgg tcctcggcga
     7021 gcacgtctcg ccggcgacgc tggaggactt cgacgagcag cgcgggttca acaagccgct
     7081 cctgttcggc cgctgggcca aaacccgcgc gtggcccgac ggccgccggc ccgatcccgc
     7141 cgcgtgggag cccggcgagc gcatcgtgcc gctgaaattc gccctgcggc cgaataccgg
     7201 ctaccgcctg gtcctcgaag cccgggcggc gggcggggat tttccaaaca ttggaaaaac
     7261 caaggtccat tttttccaat gtttggaaaa aacgggcgca ttttttccaa tgtctggaaa
     7321 aaacgacgcg gaattttcca atgtctggaa aaaaacggag atcattttcc aaacattgga
     7381 aagcacttcg gcctctgacc tgcgcctcgc cgtccagggc accccgctgg agatccggtc
     7441 ggtccaactc cggcggcgga tggccagccc gtgggactcg cagttcttcc acttcctcag
     7501 gaagatcgcg cggctggact tcggcacgtc gagcagcctg aaccagccgg tgacgcgcct
     7561 gctggcggag gggatcgggc cgacgctggc gctgacgatc ccgatcttca cgctggagtt
     7621 ggcgctggcc gtgagcctgg cgctggtctg cgcgttctgg cgcgaccggt ggcccgaccg
     7681 cctcgccgtc gtcgtcgcgg tcgggctcat gagcgtgaac taccttgtct ggatcgtcgc
     7741 gggccagtac gtgctcgcgc accggctcgg ttggttcccc gtctggggct tcgagtcgtg
     7801 gcgctacctg ctgctgccgg tcgtcatcgg cgtgatcagc gggctgggca gcagcctgcg
     7861 gttctaccgg acggtcatgc tcgacgagat ggggaagggg tacgtccgca cggcgttcgc
     7921 caagggcgtg ccgcgacgcg gcgtgctctt ccgccacgtc ctgcgcaacg ccctgatccc
     7981 gctgatcacc aacgtcgcgg tcacgctgcc ctacctctac acgggcagcc tgctgctgga
     8041 gagcttcttc ggcatcccgg ggctggggta cctcggcgtc aacgcggtga actcggcgga
     8101 cgtggacgtc gtgcgcgcga tcgtcctgat cgggtccgcc ctctacgtga tcgtgaacct
     8161 gctgaccgac gtcgcgtacg ccgtcgtgga cccgagggtg aggctggaat gaaagagggt
     8221 tcaggaggga gaagtctgtg ggccgaggcc tggacgcggc tgcgcgggcg gcggatgaca
     8281 cgggcctgcc tgtgggtgat cgccgcctac acgctgatgg ctgtttacgg cgaggtggtg
     8341 taccgcgtga accacgccct ggaccgcacg ccgtcctacc agcgcacgga tctgtcgcag
     8401 gcctaccggc cgccgtcgtg gcggcacccg atggggacgg acgggctggg ccgcgacgtg
     8461 atgcagcggc tcgtgcaggg cacgcgcgtc gcgttccagg tcggcatcgt cacctcgctc
     8521 atcgcgatcc cgatcggcgt tgtgctcggc ctgctggcgg ggtacttcgg cgggcgcacg
     8581 gacgacttga tcgtgtggct ctactccacc ttcgcgtcgg tgcccgggct gctgttcatc
     8641 ctggcgatcg cgatggtcgt gggacggggg ctgctgggcg tctacctcgg gatcggattg
     8701 acaacgtggg taggcatctg ccggctgatc cgcggcgaga cgatcaagca ccgggcgcag
     8761 ccgtacgtcc tcgcggcgcg ggcgctgggg ctggggaacg cgcgcatcct gttccggcac
     8821 atcctgccga acgtatttca tatcgtgatc gtgacgttca cgctccggtt ccctctgtct
     8881 atcggcacgg aggtcttcat gagcttcctc gggatcggcg tgcagggcga accgtcgtgg
     8941 gggacgatga tcggcaacgc gcggctgcgg ctctggcacg gggcttggtg ggaaatgacc
     9001 ttcgtcacgc tggcggtttt cgggttggtg ctggccttca acctgctggg cgacgccctg
     9061 cgcgacgcgc tcgacccgcg cctgcggacg gcggagcggt aggttttcaa ataattctct
     9121 ttaacagaac cgcgcggcgc acgtattcta taagccaata gtgctcaagg gataattaag
     9181 gaggctccca tgagaaacct tgtcgtggcc gtggcggtgt gtgtcggttt cgcgtcggcg
     9241 ctggcccagg ttccttccct gatcaactac cagggccggc tcgtgaacga cacgaacctg
     9301 gtcaacgaga cggtgggcct gtcgctgcaa ctctacgacg atccctcggc cggaaccctg
     9361 ctctacgagg actccaacca ggtcgccgtc gtggacggcc tctattctac gttcatcggc
     9421 gacgacacga tcagcggcac gctgaccggc gcgctggcgt cggccgaggt ctggatccag
     9481 gtgctcatca acggcgcgcc cctgatgccg cgtgaacagt tggtatccgc cggctacgcc
     9541 ctccaggccg cggacgtgcc gggcaacgtg ttctggagga cggacggcaa cgccgggaca
     9601 acggccggga cgcacttcgt cgggaccacg gatgagcagc ccttgcacct gcgcgccgac
     9661 aatgcgaccg tgctgcggct cgacggcgag cccaatccgc ccaacctgat tggcggccat
     9721 gcctcgaact acgcggatcc gggagtcaac ggcgccttca tcggcggggg cggcgatccc
     9781 ctgggatttt cccccaaccg ggtgagcgat cattacgggg tggtcggggg cgggatgaat
     9841 aacgtggcgg gcgacacagg cgatacggtg tcgaacagtt cctttgccac ggtggccggc
     9901 gggagtgcga acaccgccta cggcgcgtat tcgtttattg gcggcggagc catcaaccag
     9961 atattcggcg cgagcccgta cagcgtgatc gccggcgggt acaacaacat cgcccggggc
    10021 acgaacgcga cggtgggtgg cggcagcttg aacgaagccc ggcgcgccgg cgccaccatc
    10081 gccggagggg agaacaacta cgcggaaggt ccgtacgcgt tcatcggggg cggacggcaa
    10141 aacaaggcca acgacagcta cagtttctcc gccggtcaca gggcggaatc ccttcacgag
    10201 ggctccttcg tctgggcgga taactccacg ccgacggcgt ttgcctccac gtcctccaac
    10261 cagttcctga tccgcgccgc cggcggcgtg gggatcaaca ccaacgggcc gttcaccgag
    10321 gcgctgaccg tgaatggcgg catccgggcg acgggctccg tggcggcggc ttcgttcgcc
    10381 ggcgacgggt cgggcctgac gatgctccac gggacaaatc tggtgatcgg ctcggtctcc
    10441 aatgcggcga tcggcgccga cgccgtcacc ggcgacaagg tgatggacgg cacgatcacg
    10501 gacgacgacg tggcggccaa caccttctgg cagaccaccg gcaacggcgg gacggctgcg
    10561 gggacgcact tcgtgggcac gacggacacg cagcccctcg acctgcgcgc caacaacacg
    10621 cgcgtgatgc ggctggacgg caactataat ccgccgaacg tcatcggggg cagtatggcg
    10681 aacaccgtgg acgtcggtgt ggccggcgcc acgatcggcg gcggcggcga tcccgccatg
    10741 gtgtttatca accggatcag cgacgattat gggttcgtcg gcggcggctt gggcaaccgc
    10801 gcaggcaaca gcgggggcac ggccgccgat gcggctttcg ccactgttgc cggcggcttg
    10861 aaaaatgtgg ccaacggtct gttttctacc atcggcgggg gggatatgaa tgccgtgacc
    10921 acggtggccc agggcggggc gatcgccggc ggcgagacca acgtggtgag cggccggaac
    10981 gccttcatcg gtggcggcaa gttgaacgcg gccacgggga gttggtccgt ggttgccggg
    11041 ggcctggcga accgggccga agggcgcggc agcgccgtcg ggggcggctc caacaacgtc
    11101 atcaactccc aggcttggga cagcatcatc gccggcggtt tcaacaaccg cgccgacgag
    11161 aatcgcgcct ttatcggcgg cggacagggt aactacagca ccggccagat gtcggtcatc
    11221 gccggcggct acatgaacac gaacatggcc gtcggcgccg tcatcggcgg cggcgggctt
    11281 aacttcaata ccggcgtcta tgccaccgtg gcgggcggac tattcaataa ggcgctgggt
    11341 ccaaacgcgg tcataggggg tggattgcgc aacgaggcgg ccgggtacgc ggccgtggtg
    11401 gccggaggca ccaacaacag caccttgtcc ggcatgacgg ccatcggcgg cggcggcctc
    11461 aatttcatcg gggtgaacgc cgagtatgcc acgatagccg gcggccagag taatacggtc
    11521 aacgacactt ctccgggcgc cgtcgtcggc ggcggaagga acaacgtggc aggcggctct
    11581 gacgtcatgg tgggcggagg acaggccaac ctcgccgcgg gcgctttctc cacgatagcc
    11641 gggggcagct acaacaccgc caacggcatg ggatccttca tcggcggcgg caccaacaac
    11701 accaccgcgc cttgggccgc gtattccgtc atcgccggcg gagcggccaa cgtaatggac
    11761 acccacgaat cctttatggg gggcggctcc accaacacca tccggaccaa tgcctaccag
    11821 agcgccatcg tgggagggaa cggcaacacc atcgagacca atgcctaccg cagcgtgatc
    11881 gtcgggggat accataacac catcctggcc tatgccgacc acaacgcaat tgtcggaggc
    11941 atggcaaatg tcttgggtgg aagctacttc ggatttatcg ggggcggaaa tgaaaacgag
    12001 atccagggcg attatgatgt cctggtgggt ggcaacgata actatatcag cagccaagcc
    12061 aactatgcct tcatcggagg aggcagcggg aacatcataa cgaactttta cggccacgga
    12121 tcgttcatcg gaggcggata ttacaacagc gtcagcggac gctggtcgtt tgtcgccggc
    12181 ggctacagaa acctgatggg cggcgaatac ggcgtgatcg ccgggggcag cagcaacgtg
    12241 gtcaacggcg actatgcggt cgtgccgggc ggccgggaga atatcgcctc ggccccgtac
    12301 agcttcgccg ccgggcgaat ggccagcgcg gtacacacgg gcgcgttcgt ctgggcggat
    12361 tcgacgggca gcacgttcag ttcgaccacg aacgaccagt tcgcgatcca ggccagcaac
    12421 ggcgtgtaca ttgcccagga cctcggcagc gaccgcaaag ccaagttcgg cgagcgattc
    12481 agggacaact ccgtggtcgc ctgggccaag gtggacgacg acgggagcct gttggcaagt
    12541 ttcaatgtcc acactgtgac gaacgcggcc gtgggacgat acgacatcct gctgaaatca
    12601 ggcgcgcgcc tttccagcgc ctgtattccc gtggcgaccg ccgaacgcga tggtcaaccc
    12661 gccagcgccg cgacggtccg cctggtcagc atagaccaga cggccaccaa tcacttccgc
    12721 gtctacatca acgacgggac cttcgcgccg gttaataacg aattcacggt ggccgtcttc
    12781 ggtcggtagg ccgtcgtgat agggtaagcg accgagcttg acgcccggcg ttccggcgga
    12841 tagactgccg cccgaacgtg ttaaggagaa ccagcgggtg tctacgacga tagaaaggct
    12901 gttgatcatc caggaacgtg accgtgccat cgcccgcctt gtccgcgaaa ccaaggacat
    12961 ccccgcccgc aagaaactga tcgaggatcg cctcaaggcc caccaggacg ttttgcacac
    13021 cgcgcaggag gacctgaaaa aggcccaggc cgggatcaag caggtcgaaa tcgaggtcga
    13081 cagccgccgc cagaagatcc agcgctttcg cgagcagcag atccagatca agaagaacga
    13141 ggagttccgc gccctcgagc gtgaaatcgc cgcggtccag aaggaaatcc gcgccgagga
    13201 ggaccgtgag atcgagttca tggagcaggt cgagaaggcc cgcgccgtga tcgccgagcg
    13261 cgaggcggac ctgaaaaagg aagacgcccg cgtccgcgag gaccagaagg tgttcgaccg
    13321 ccggctggaa gagcttcaag ccgagatcgc caagctgcaa accgaccgca aggcgctggc
    13381 cgagcaagtg gacgcgacct ggctgggccg ctacgagcgc atcctggcca agaccggcga
    13441 ctatgccctg gtgcccgtcg aacacggcgc ctgcggcggc tgccacatga acctgccccc
    13501 gcacctcatc cacgaggccc gcaagggcct gtcggccgcc cagtgcagct actgcagccg
    13561 gatgctgtac tggaagccgt agtgtagcgc cgcacggtgt gcggcggttg cgccacgccg
    13621 tggcgcacta tagccgtccc cctcgatatc catcgctttt tcacccgccg ggagtatagt
    13681 gattccggag ccggggcgga cggtcgctgc ggtcccgccg cgaggcgggg ccggggagga
    13741 aagtccggac tccgcagggc gggatgtccc ctggaaacgg gggaggccct tcgccatatc
    13801 gaagggcacg gaaagcgcca cagaaaatat accgccccgg gccgccgaaa ggcggaacgg
    13861 ggtaagggtg aaaaggcggt gtaagagacc gccgcccccg gcggaaacgc cgggggcagg
    13921 gcaagcccca tccggagcaa gatcgaatag ggagcgagag gcggcccgcc tcgttcgtcc
    13981 cgggcaaccg ggacggtaag ctccgggttg atcgcgcaga taaatgaccg tacaccccga
    14041 cgtggtcggg gggacagaat ccggcttaca gccccggctc catttttgtc acgccttttc
    14101 aaggcgatcc ctgtattttg tcgcttccgc gaaacggaca atctagagta ggctgccata
    14161 tgaggggctt gtccatcgcg cgctgggtgg cgctgctcgt ggccgtcgcg gcggtcgcgg
    14221 gcttcgccct gctgtaccgc cgcctgccgt cgagcccgcc ggcggccgcc gtcccggagg
    14281 ccgtcttggc gtggccttct ccttctgcgc cggacgccga agactgggcg gtgttccagc
    14341 ccgccgcggg cgccgcgccc gcggagttga gcccgctcgc gcgccgcttc cgcctggccg
    14401 gcacgttttt ttccatcggg gaggagcagg acgaacggcc cccttccgcg cggctggcga
    14461 tccttgacga cgtggaaaag aaacagcagt tcgtcgtccg cgaacaggag accttcgacg
    14521 acgtccaggt cgtgcgcatc ctccaggacc acgtcgtgct gacctcgcac gggcagcagg
    14581 aggagctgtg gttgagcttc cacggcgagg ccgcggaatc gccgccgccg gccgcccccg
    14641 ccggcgaggc cgtcgagccg acgctggagc agaaccgctt cgggcggcgg gtcggggaga
    14701 cgcgctgggt attaagccgg caggcgctga tggactacta ccaggaactg gccaacgatc
    14761 cggagcgcat cgcggccctc tacatgacca tgaagccgga ttacaaggag aaccgcatcg
    14821 cgggctacca ggtggacatc gagggcgagc aggagtttct cgacgccgtc gggctgaagc
    14881 agagcgactt catccgcaag gtcaactcga tgaacatgac cagccagcgc cgcgcggaat
    14941 acttcatcag cgagttcctc caggagcggg tcagcgctgt ggtgctcgac atcgagcgcg
    15001 agggcaagga gcagaagctg atctatctca tccgctgacg ccgtttctcg cggcgcctgg
    15061 ttcgcgcgtc cgccgacgct tcctccagca ggagtccgac cgatcgccgc cgcgcgtcca
    15121 cgcgggccag ccggacgcgg acctcctggc ccacgcgcca cgaccggtcc cgccgccggc
    15181 cggtgatctt catgcggtcc cggtcggtca cgtagtggtc ctcgggcagg gcggagaagg
    15241 ggaggtggcc gcgttgcagc gtgtcgcgca gttcgacgac caggccgcgg gcgtgcagcc
    15301 cgatgatcac gccctcgaac ggacccgtct cgtcgcgcgc catgaggccg tgatagtagt
    15361 ccaatcgctt gcggacgatg ctgtccgatt ccgcctcgtc ggcctgggct tccgtctgcg
    15421 aacagtgggt cgccaggcgg gcagtatcgt cggcggaata tgggggcgcg gcgcgcgtct
    15481 ccagggcctt cagcacgcgg tgcaccacga gatcgggata acgccggatc ggcgaggtga
    15541 agtgcgtgta gcagtcgaac ccgaggccga agtgcaggcc gggcaccggg gcgtagacgg
    15601 cgcgcttgag gttccggaga atggcgagct gcgcggcgta ggacatgggc gacgcggccg
    15661 cgagccgggc caggcgggac aaggtatcct tccgcggcgg gagcgaagcg agtcccagcg
    15721 cccgtagctc gacctgcatg cgctcccact gcgcggcgtc cggctcgtcg tggatgcggt
    15781 agagcgaggg ggccttctgc cgggcgatcc ggcccgccac ggcctggttc gccgccagca
    15841 tgaactcctc gatgagctgg taggcctcgg tcgaaccgcg cttccggatc tcgagcaccc
    15901 ggccggcgct gtccatccgg caacggatct ccggcatcgc caggtccagg ctgccctccg
    15961 ccatgcgccg gcgtcgcagc aacgcggcca actcgcgcat ctccgccagc gacgcccgga
    16021 cttccgccgg gatggacccg cccgcgccgc cgtcgaagca cgcctgcacc tggtcatagt
    16081 ccaggcgggc ggccgaccgg atcaccgagg ggccggtcga gaaatccagg acgccgccga
    16141 gcggatcgaa gaggatgaac acggaatggg tcaggcgcgg cacgcccggc tgcaagctgc
    16201 atacatcggt ggtcagccgg ggcggcagca tggggatgac gcggtcggcc aggtagacgc
    16261 tggtgcctcg gcgccgggct tcgacgtcca ccggggaatc gaccgggaca tagcgggaca
    16321 cgtccgcgat gtgcacgccg agccgccagc gcccgtcgtc tgcgcggacg agggaaacag
    16381 cgtcgtcgaa gtccttcgcg tcctccgggt cgatcgtgaa aaccagctgc ccgcgcaggt
    16441 catgtcgatc gtggaacagg gcgggggccg gcgccggtga ctcatggtcg gcctgccgga
    16501 gcgcctcggc tggaaactcc gaacgcacat cgaacgagcg catgacggac agcatgtccg
    16561 cgccgggcgc atcggcgggg cccaggtctt ccacgaccgt gccggacagc gactcctgct
    16621 cgcgttcccg gggttccagg tccaccacga ccaggcgatc ctcttccggc tgcgccaggc
    16681 cgggggcgaa cccgcgcacc cgcacgttct cgcggatccg gggattgtcg gggatgacgt
    16741 acccatagta ggccgtgcgc cgcagccggc cgaccacgcg ctcgtgcctt cgttccagga
    16801 cacggaccac gcggccgcgg gtccgggcct cccgctcgtc cgggacctcc ccctcggttg
    16861 aaagggcgat cagcacgcga tcgccgtcaa gggcgggccc gagcgactcg gcggggatgt
    16921 agatgtcggg ccggccggtt tgctccgggg tgacgaaacc gaagccgtcg acgctcacgc
    16981 ggaggcggcc ggccagggta ggggcaccgc gtggcgcggc ccagcgattc ttccgcaacc
    17041 ggacgatcaa accctcccgc tccatgtcgc gcagggcgcg ccgcaggaca gggccggacc
    17101 aggccagggc ccgcgccagt tcatgctggc gaagcggccg gtagtccggt cgatggagaa
    17161 aatcgagtat ccgtttccgt tcggatggtg taaattctgc catagagata gactatatcg
    17221 ggtttggcgc ggcaggcgag cggaagcggg gcgggttcct ggagaaatga aatgagggtt
    17281 ctttacgcgg ccagcgagat catgccgttt gcctccacgg gcggactcgc ggatgtcggg
    17341 gcaggccttc cgaaagccct gctggcccgc ggcggcgaca tatcgagaat catgcccctc
    17401 taccggcgcg tcgcggaagg cggctatgcc ctcaaggata ccggcctgcg gctggacatc
    17461 ccggtaggat tccagatgca ccaggccgag gtctggtaca ccgaagatcc gccgccgcgg
    17521 acctatttca tccggcgcga tgaatttttc gaccggtccc agctctacag cctgcccgag
    17581 cgcgactacg acgacaattt cgagcggttt gtcttcttcc agaaggccat cgtcagcctg
    17641 atcgataccc tgcaatggca tccggacatc gtccactgca gcgactggca gaccggcctg
    17701 ctgccgcttt tcctacggca cggcatccag ggatcgggcc gtaaacagac ggagcgcacc
    17761 atctttacca ttcacaacct cgcgtaccag ggcttgttcc ccggctcgca gtactcgcag
    17821 acaaacctgc ctttcttctg cttcaacatg gattgtgcgg agttttacgg cagcatcaac
    17881 tgcatcaagg cgggcatcac ttcggccaac ctcgtcacaa ccgtcagcca gacgtatgcg
    17941 caggaaatcc ggaccgagga atacggctgc ggcttgcacg gtattctggc ccagatgggg
    18001 gaccggctgg tgggcgtggt caacggcgtg gactactcgc tgtggaatcc gaaggtcgat
    18061 cctcaccttg cgaaggccta tgggccggac gacatatcgg ggaaaaagga ctgcaaggaa
    18121 gaactgcttc gggtgatggg tcttaccatc tccccggagg cgcccttgat cggcatggtc
    18181 agccggctcg ttgaccagaa aggccttgac atcctgacgg aggcgatgcc ggccctcatg
    18241 gaaagcaatg caggctttgt gctgctcggc gccgggcagg ataagtacca gaaactgtgc
    18301 ctcgaatggg cgtcccggtg gccgggccga tttgccgccc gtcttggatt cgataatcct
    18361 ctttcccata agatcgaggc gggagcggac atctacatga tgccgtcacg atttgagccg
    18421 tgcgggctga accagttata cagcctcaag tacggcacga ttcccgttgt gcatgccacg
    18481 ggcgggttgg acgacaccat acaggacatt gatacagagg gccatgaagg gacgggcttc
    18541 aaattccgaa cctacacggc cgagggcttg gctcacgcga cgcagagagc gatagccctt
    18601 tatcagcgga gcgatttatg gccatcggtg attcgccggg ccatggccca ggatttctct
    18661 tgggagaggg ccgccgagga ctaccagaag cggtatcagc aactcatgtc ctgatccatt
    18721 tgcaggattc tgtccgttaa cttcgaacgt cgcacggccg cgcttccgat ccattttcca
    18781 ccagctattg tggttttgcc gatcctttag tagacataag atatattatg cgacgttaac
    18841 tatgaggccg ccacgggcgg gtttctggca agcgaattcg aggcaaggcg atcttcctgg
    18901 catcacacgt ggttgggttg ccaaggacgc tgattgaccc tcgctggtcg gaccatgcca
    18961 ggcatggcga gaggtcttcg tccccttaca gtcggacgcg ggtaagaccg cgctgtttta
    19021 ctgcacgcgg ccccagcgcc gggtaaaagg ccagtaaacc atgaaggcgg ggccgaccag
    19081 gtactctttc ttcaccggcc cgaaataccg gccgtcgtaa ctatgctcgg tattgtcccc
    19141 catcatgaga tactcatcgt cgcccaggac gcgggattca ccctgcacgc ccaaatagac
    19201 gggaatagcc gcctggtccc gggccagact ataaccgaca tatcccttgt cccgctcgtt
    19261 gacgagacgg gcaaaaggat agggctcggt gatctttttc ccgtccacca ccagatacgg
    19321 cggatcgatc gccacggtct cttccggcag gccggtcatg cgctttatgt aaaacgagtt
    19381 gggccggatg ttcggatatt ggatcccgtc cgtgctgaac acggtgatgt cgccccggcg
    19441 cggccgtgaa aagttgtatc gcagcttgtt cacgaagata tggtcgccca gcttgacgcg
    19501 tcccgaggcg agaacctggc ccttggccac gtaatcgccc ggcgcgaaga agagcgtcat
    19561 gcccttgcgc acgggatgcg gcacgtcctg cacgtagaag acgatgtgtt cttcctcgtt
    19621 tccgaactgg ctgccgacct gcccggacac cttggccttg acctccgtgt accgctcgcc
    19681 gaacagcgcc atggaaacaa gactgacagg aaagcggtcg tgccacttgg gagcatactg
    19741 tgtcccgacc gtgatcccgt acagcgtcgg ctgcatggag cccgtcggga tcttgaacgg
    19801 ctggatgaag tacgtgcgga accccatggc cacggccagg gcaaccgtga tgatctcgac
    19861 gtattccgtg atcttggcaa agcgccgcgg cgggttgatc cgcaggatgt attcggacag
    19921 ttcctcggcc gcctcctcga cgtgctccgg gtggcgctgc ttccacgcct cctggagtgc
    19981 ctgcgtccgc cgggccagct cctccagcgc ctccgcaggc aggatgtcgc cccgcacgtt
    20041 gaccacgtgc cgggactcct gcacgaggtg atgcacccac tggcggtacc gacggaattg
    20101 aaacaggttc atggtccttc gcctagtcgc tggtcttgag cacggaaatg aacgcctcct
    20161 gcgggatgct gacctgcccg atctgcttga gcttcttctt gccttccttc tgccgctcca
    20221 gcagcttccg cttccgcgtc acgtcgcccc cgtagcactt ggcggtcacg tccttccgga
    20281 acgcccgcac ggtctcccgc gcgatgatct tcttgttgat cgccgcctgg atggcgatgg
    20341 cgaacatgtg cggcgggatc agctccttga gcgatttgca gatctgccgg ccccggtatt
    20401 ccgccttcga ccggtgcaca atggccgcaa acgcctcgac gacctcgccg tgcaccagga
    20461 tgtccatctt gaccaggtcg gactcccggt acccggcgtg ctcgtagtcc atcgaggcgt
    20521 agccgcggct ggcgctcttc aagcggtcgt agaaatcgat caggatctcg ttgagcggca
    20581 cgctgcacgt cagcatgacg cggcgcgtat cgagggactc ggtcttggtc acctcgccgc
    20641 gtcggtccat gatcaactgc atcatgtcgc cgatgtgctc gttgtggcag atgatgaacg
    20701 ccttgatcat cggctcctcg atccgctcga tcttggtcag ctccggcagg tgcgcgggat
    20761 tgtccacctc gaccattgcg ccgtcggtca ggtacacgcg gtagatcacg ctcggatacg
    20821 tggcgatgat gtccacgtcg tactcgcggc gcaggcgctc ctggacgacc tccatgtgca
    20881 gcaggccgag gaacccgcag cggaacccga accccagcgc cgccgaggtc tcggcctggt
    20941 ggacgaaggc gctgtcgttg aggctgaact tctcgatgct ggcccggagc ttctcgtagt
    21001 cgtccgactg gaccgggtag agcccggtga acaccatcgg atggaattcc ttgaacccgg
    21061 gcagcggctg cggcgcgggc cggtcggaac gcgtgatcgt gtcgccgatc tggatgtccg
    21121 acgcgttctt gatgttggcc atcaggtagc cgacctcgcc cggctcgagg gtcggccgca
    21181 ccaccggctt cggggtgaag atcccgactt ccttgacctc gtagtcgcgc gcgatgctca
    21241 tcaggcggat cctgttgccc gggcttaacg tgccgtcgaa catgcgcacg tagatcacgc
    21301 cgccgcggaa cgtgtcgtat tccgagtcga agatcagggc ccgggtatgg gcttcgtcct
    21361 tttccaccgg agacgggatg cgctggacga tcgcctcgag gacctcggcc accccctgcc
    21421 ccgtcttggc gctcacctgg aggctgtcgc ccatggggat ggccaggacc tcctcgatct
    21481 ggtgcgccac cgccaccacg tcggcgttcg gcatgtcgat cttgttgagc acggggatga
    21541 tctggatgcc gtgctccgcg gcgagcagcg tgtgggcgac ggtctgcgcc tcgacgccct
    21601 gggtgacgtc cacgaccagc agcgcgccct cgcacgccga aaggctccgg gagacctcgt
    21661 aggagaaatc cacgtggccg ggggtgtcga tgaggttgaa gcaataggtc tgcccgtccc
    21721 gcgctgtgta attcatggtg acgggatgcg ccttgatcgt gatgccccgc tcgcgctcga
    21781 gatccatgtc gtcgagaacc tggtcgcgga actctcgctt ctggatggtc ccggtcagct
    21841 ccagcatccg gtcggcaagc gtggacttcc cgtgatcgat gtgcgcaata atgcagaagt
    21901 tgcggatgag ggcgagttcg ctcatggatc agccccgacc cgccagggac ttttggatgg
    21961 ccgcccgcat ggaacgaatc cgcgccgcca tgtcgccgtc ggccgtgaaa agcgaactgc
    22021 ccacgagaaa gatgtccgcg ccggcctcgg cgcagcgggc ggtggtttcg tcgttcaggc
    22081 cgccgtccac cgagagggcc atgcccggca gcgcctcgcg caggccccgg attttcggca
    22141 gcacctcttc caggaacgac tgcccgccga atccggggtg cacggtcatg cagaggatct
    22201 cgtccgcccg ctcccggtac ggcagggcgg cctcggccgg cgtttcggga ttgagcgtca
    22261 ggcccgcgcg ggcgccccgt tcccggatgc ggcccagcag cgcggccggg tcgcatcggg
    22321 cctccacgtg gatcagcagc gtgttcgcgc cggcgtccag gaaggcgtcg gccagcccgt
    22381 ccggccggct catcatcaga tggacgctca acggcagttt cgtca
//
LOCUS       DAOOTK010000043        22242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1601_length_22242_cov_4.738450, whole genome shotgun sequence.
ACCESSION   DAOOTK010000043 DAOOTK010000000
VERSION     DAOOTK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22242
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_22242_cov_4.738450"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK634_06770"
     CDS             <1..358
                     /locus_tag="PK634_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97476.1"
                     /translation="DAIALRALQRLKERGLIEKAEIEPFYVELTGIARRYLESRFGLR
                     APERTTEEFIREASVSGLLSAAHQALVRDFLEQSDLVKFARFAPGIPEMNAAFASAEK
                     LVVETRPAPATQGGPA"
     gene            355..1350
                     /locus_tag="PK634_06775"
     CDS             355..1350
                     /locus_tag="PK634_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOW97477.1"
                     /translation="MSLQHPWFLLLLLLVPGLVWWRHGRARRPTLQFSDGRALGRLPV
                     SWAVRARWALPVLYALALFCLVVALARPRRGLAESRVRTEGVDIVLLSDVSTSMRAED
                     LSTATRTMNRLDAAKAVMEAFIQARPDDRIGIVAFAAMPYTLAPLTLDHGWLVQQMGR
                     LETGMVEDGTAIGDAIAAAINRLRDSKARSKVVVLLTDGMNNRGVLSPENAAEAARAM
                     GIKVYTVGAGGDEPAPVPISSPFGGRQYAFQPSDIDEPMLKYVAETTGGLYFRARNFK
                     GLDDVYRQIDEMEKTEVDVEHYTRFEERFMPWVIAALVLLGLERLLGLARLEAAP"
     gene            1350..2369
                     /locus_tag="PK634_06780"
     CDS             1350..2369
                     /locus_tag="PK634_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOW97478.1"
                     /translation="MKWGAPELLFLLALLLPAAWLVFFLLRRRERRLARLVDGSQFAL
                     LAPGRNPQRVRTRHLAWLGALALMLAALARPQWGTRWEEVRRRGLDVLVVLDTSKSML
                     AEDIKPNRLQQAKWGIRDLLRQLRGDRVGLVAFAGSAFLQCPLTIDYPAFLMTLDDVY
                     VGLIPRGGTAIAQALRTAMDSFEEDAGSDRALILITDGEDHEGKPLAMVPELKKRGIR
                     VFAVGVGTLEGELIPAPDGSGSSAFLKDRENRVVKSSLNEDLLARLAVETGGMYVRST
                     AEDFGLDRIYEKGLSELRRDEHEGRMVRMHEDRFPWALGGAFILLAAESLLRERPRRG
                     KERPA"
     gene            2366..3235
                     /locus_tag="PK634_06785"
     CDS             2366..3235
                     /locus_tag="PK634_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97479.1"
                     /translation="MKNILLASLLLSALRAGAADDPRAQLRAGLREYEQKQYESALQQ
                     FEGAAAGAATNELDAAVPAYNAANALLQLDRADEASQRYQDALRSPDLGLQGRAFYNR
                     GNSLYRLAEAAASAPGPAMPLPAAPPAATTPPTAPQAPGLDTAGQAVEESLKMYERAM
                     FLDPADEDAKANYELALQLKEKIQQMQQQQQQKQQKDQPQQQDKKEQEQKEKEQEQEQ
                     EQEQEQEQEQEKKQGQEQKQEQAAKPEPTPAEQMTPEEARLLLDRMKQEEQSYRDKLQ
                     VIMGRPVPVDKDW"
     gene            3241..5070
                     /locus_tag="PK634_06790"
     CDS             3241..5070
                     /locus_tag="PK634_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOW97480.1"
                     /translation="MARRGSIRAALIGGAALAAAAGQAAFAVTADMDVNPRAVSVGES
                     AICNMTVHGLDNPPPPSLPAVPGLQISPVGTERSWSMGPGGQDSSVSFRFRVLPLQAG
                     KFSIGPIAYEAEGQRMDIPAVELEAVDQGAAATSDGGGSPIADLLFATISAQPTNVYI
                     QQVFDVVLCIYSRNITLGRDVELGNMPASGLSLPPFQEMGSTREVVRNQIYDVRRFRC
                     KAQALTAGAFTLEPNIRVQVVVPRDRRRMSPFDDLFNDPFGNPFMGRYQTQAVDLRAE
                     PVVLNVQPLPPADGVPGFAGAVGRFAFDVQVKPTEVSAGEPVTLSMQIGGVGNIENIS
                     PPAIAAGPDFRLYEPRQVSRDIDGARSGGRVLYEQVVIPRSDTNAQLPALSFSYFDPE
                     LGRYETVTRGPFDLVVHPATNGAGRVIQAAADVPEARTMILGSDIVYLKPPPPRWPRP
                     GNAPSIRAVFWGVQAVPALAVLGLFLAARRREQMSRDVARARRARAPRSARPALHRAE
                     KALAGDRRGEFVDALQEALAAYLGNRLNLPAGAVTPDVIMARLEKSALPADGIARARD
                     LLAFCERERYGQGSGHVEGDRALLAARLEGLRGILKACERVRL"
     gene            5070..5873
                     /locus_tag="PK634_06795"
     CDS             5070..5873
                     /locus_tag="PK634_06795"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97481.1"
                     /translation="MDIRAPCRIALAALLAFAPRPAAAQPSDEWPAAKAQQMFLEAGQ
                     AYDQGRLADAVEAYETLRGRGLRFQELFFNLGNAYFRQGDLGRAIVNYRRAWVLNPGD
                     PDTRANLRYALQTAGVPEPALRGTQRFLFRASRETWRWIAVGAWWLAAGLAGAYLWSR
                     SRPAWMAKLAVALLAVMLVAAWGALAWDRLCRAPEIVVEGKGHEALFAPLAGSTAHFA
                     LPEGSIGRVVEHSGNWIRIASGGQEGWIQAEHGLPVCYWLVENSLFSHD"
     gene            5976..6110
                     /locus_tag="PK634_06800"
     CDS             5976..6110
                     /locus_tag="PK634_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97482.1"
                     /translation="MALLLSVDRTNISARLAARATILLTGSAMALCLICGTADWWLKR
                     "
     gene            complement(6126..7235)
                     /gene="alr"
                     /locus_tag="PK634_06805"
     CDS             complement(6126..7235)
                     /gene="alr"
                     /locus_tag="PK634_06805"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOW97483.1"
                     /translation="MKTSWVQVDLKRLAENIRAVRLALRPPTEILFVVKANAYGHGLV
                     PVAVHAARHGVRWFGVAYLHEALQVRQVLPDVRILVLGATEPSDVPLLVGRRITPVVI
                     SEKHAVELGQAAKSMGKTLPVHVKVDTGMGRLGIWWEDAGAVLDRLGAPCGLEIEGLC
                     THFATVEPRKPAPARVQWERFAAASAAVEQRVGGRLFRHVSSSRAALYFRDWDVDAVR
                     PGIVLYGYGAADPALRFHTKPILQWKAHVMQVKRVPAGFSVGYYSQFTTTAPTALATL
                     SVGYADGYNRLLGNKGVVLIGGWRCPVVGRVSMNWITVDLGHDRTAQEGDEAVLIGEQ
                     RSESIWAGELAAACRTIPYEILTDINAALDRRYVE"
     gene            7340..8335
                     /locus_tag="PK634_06810"
     CDS             7340..8335
                     /locus_tag="PK634_06810"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOW97484.1"
                     /translation="MGARIQSVSTDGRAVEQAAACLRAGGLVVFPTETVYGLGADALN
                     PKAVARVFEVKQRPRFDPLIVHIADIEWLRDIAAAFPPAARKLADRFWPGPLTFVLPK
                     TDRVPDLVTAGLPSVAVRMPRHPLALDLIRQVGGPVAAPSANRFGHLSPSTAEDARTE
                     VGEGADMILDGGPCPVGIESTIVSLLDPNPLLLRPGGLPLEDIESVIGPLARARHADE
                     RPQSPGRLLRHYAPRTPLSLVSAVPPRADRAGAGLLSPGPVPEAGGFAMVEMLSPALD
                     AAEAAARLFSALRRLDAAGLSRIYALEGPERGLWRAINDRLRRASVRDAPDEIPG"
     gene            8332..9471
                     /locus_tag="PK634_06815"
     CDS             8332..9471
                     /locus_tag="PK634_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW97485.1"
                     /translation="MKKALNLGAALAVLVCLGGIFAWSEFVPALRKAYGYTAAQTQWI
                     FGLTIASFTTSMIGAGRLLYRWGPARVAATGGLLFAAGYLVGAFTCDRFAGLLAGLGL
                     LAGCGIGFGYVAPLATVMAWSPGRRGLAGGIAVAGFGSGAILLASLAAAGWRHGLEVS
                     RLFGIIGLSYGAVILLAAAGLSRPPDSVVSAGAGRARPWKDARLRRLFLGMFCGTFAG
                     LIMVGNLKPIALSGGIAAGAATASISILAGAVLLLLPARASGPLFGLGAGLIGLGFGA
                     CFVVYAAEVAAHYGAGQVGGVYPLVFLAYGLAGLTGPALGGWLFDVSGTYRYSMLLAA
                     AVAASGAALARMPAGRGAPTAPAFPSCHRPLFFTDSSIADRTKGT"
     gene            9474..10397
                     /gene="speB"
                     /locus_tag="PK634_06820"
     CDS             9474..10397
                     /gene="speB"
                     /locus_tag="PK634_06820"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOW97486.1"
                     /translation="MTDSSHFQPAWNFLGLPDELAAPERARAWILPIPYEGTTSYGAG
                     TREGPSAIIAASRQVELFDREFDGEPAMAIGIHTMNPLGLEHGSPEAMVAAIENAVAG
                     ILSGTPRPEQLVVLGGEHSISGGVARGMKKAGLKDFVTVHVDAHADLRAEYEGTPYSH
                     ACAARRIVEVSPIFQVGIRNLSEGEERFRRGNKRIRTVFSEEANAGRGWLKELAKFVK
                     GKQVYFTIDLDGLDPSIMPAVGTPEPGGLSWERLLDVARVVCREAADVPVFDVVELAP
                     IPALKGPDFLAAKLAHKIVALSLYRRPARRR"
     gene            10415..11587
                     /gene="speY"
                     /locus_tag="PK634_06825"
     CDS             10415..11587
                     /gene="speY"
                     /locus_tag="PK634_06825"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOW97487.1"
                     /translation="MSKITLPKTFISKYPHKFAKGKTRFLSGKRILPPPITGKESVPE
                     LVNRTFLAYNAARLQEGCRLFAEKMLANDDVTVGMSLAGALTPAGLGAAAVVPLIKAG
                     FVDWIVATGANLYHDMHHAMNLPLYKGTASIMDPVLRRLGVVRIYDIFLDYNDVLMRT
                     DDILRKILIQPEFQKEMGTPELHYLLGKYCAEFEKQTGLKDVSVLAAAYRAGVPCFCS
                     SPGDSTIGMNVAGVELRGNKLRVNPSICVNESTAIVLDAKQRRKKTGVVLVGGGSPKN
                     FTLQTEPQIQEVLRIKEAGHDYFLQFTDARPDTGGLSGATPSEAVSWGKIDPNQLPDA
                     VVCYLDSTVGLPMLTHYALAQRKPRKLKRLYDKRGAIMARLTKEYFKHNPDATRIG"
     gene            11601..12743
                     /locus_tag="PK634_06830"
     CDS             11601..12743
                     /locus_tag="PK634_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOW97488.1"
                     /translation="MPSPALLKQLAKKHGTPLFVVDHDVLRRNFAEFRKGLPRVQPYY
                     AVKANPDTAIVRTLFEAGASFDVASMPEFRIAYENIRKMPPKPRQHWIWDKIIYANPI
                     KDNDTLRQLNPYKPLVTYDNLEEIRKVRKYAPHAGLMLRLNVPNTGSVVELSSKFGAS
                     AGEAVDLIAAAFKAGLVVEGVSFHVGSQCANFQNFVQALAIADGVMKEARSRGYAPVK
                     ILDIGGGFPAPYDRHVRPFGELAAVLNAEFDRLFPPDMEILAEPGRFLVATAATLVVE
                     IIGKSRRDGKLCLFINDGVYGTLSGVLFDHCQYHLKAFRKGPTEICSVFGPTCDALDV
                     VSVAEDLPTNLKLGDLLYSENVGAYTTASATHFNGMPLARIVALNR"
     gene            12826..13851
                     /locus_tag="PK634_06835"
     CDS             12826..13851
                     /locus_tag="PK634_06835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW97489.1"
                     /translation="MDTKPKRRLVWLTRTFLLLILGLLAAEETCRLLPAPVSLPVDKT
                     RISFWADQDRQHPWLTNGSNALRVAMLGDSITAGAGVRGQVIFGGQLEWLLNLNRDTP
                     PAMVGVFAQAGTSPVNQKFLLRKALDIQPSLLILGTTVSDLEDRKVPEPLMSLMAATR
                     PRDPPPDMTGLARYSCALARWARAREKRRVHEATRQYYDFLFREDYSGWVSYCYAVRA
                     FRDACRARQIEFMVLVLPPMNDVREHAESLDKHHRMLRRFLAGEQIPCLDLLPLLREY
                     AAERLELVPGVDSHFNELGHRVIAENLFAYLLANNLIPQTYVPQHMSHPSLARLWGAH
                     QPRCPAP"
     gene            complement(13930..14003)
                     /locus_tag="PK634_06840"
     tRNA            complement(13930..14003)
                     /locus_tag="PK634_06840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13967..13969),aa:Arg,seq:tct)
     gene            14105..15202
                     /locus_tag="PK634_06845"
     CDS             14105..15202
                     /locus_tag="PK634_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOW97490.1"
                     /translation="MRKLGAALGMLVAAGFLGARAATLEIRVTGLSTSGPVRAAVFAS
                     REAYEKKEPLREMTAEPDAEGAPFHFEGLPPGEYATRVFQDLNDNSSLDRHGFLPAEP
                     FGFSGNFLPRLGPPSFSDLAFRIGEEDVRETISLIPPPRRGEWSAGAALIAKTESYRG
                     MDDRVIPLPFVSYAGPRLHWFGPVASLAVYESGGFSVAGTARYTFDGYEQDDAPIFED
                     MPDRDATLEAGARAGWAFAPRWRIDTSVAHDLLGRHGGFRAGLGLRRTFNAGELTAVP
                     EVALEWMDDAVAGYYYGVQPAYAREDRRAYAPDDAWEPRLGISLLYPVRPKWILSLRL
                     AARLLPDELTDSPLVGDDEEITAFMSVSRSL"
     gene            complement(15232..18600)
                     /locus_tag="PK634_06850"
     CDS             complement(15232..18600)
                     /locus_tag="PK634_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOW97491.1"
                     /translation="MRALVRYPVKAACIALALSALSGRASIQTNGPVSSVQVVTNAAS
                     GNRSFIFHLSPTGAVEVAPHAADAVRVRFHWDSLWDKEEVGIAKPFSNWPAFSVNFSE
                     SGSTQFVITSSQLRIEVQKTPVFNIAFRDPSGYELLRARRMEYDWNYQPGSDSSYEMV
                     NWGSVSVSNLPNGFKLKAVMEMPADEAYFGLGEFAGPLNRRGYNIQGWNQDTYYWQES
                     RNPMYMTLPMYYGMRPATNGTPARAYGVFFNNPSRPTFRMGTQWGDAYSFEAGDGQLD
                     YYFFGGGADHAPLDVLNRYSELIGYPAKMPRWAYGYHHSRHSYWVQHQVHTLLDQFRQ
                     YGVPLDAVYLDIGTQDNYHQMTFNAGYTNVAGLIQYAASKGVRVVPLIEPLLKTSDPM
                     YSEAYSSLHFLKANDLSTYVGTNFLGNVSWLDFSSSFARDWWKAKMTNYLHQYPFPGI
                     WNDLNEPNENAMALNVVYYCDDKYGGGTNNNDSRKWHQVNKNTFNVRQTSLTYDTLKT
                     RDPVGRPFVLSRAGWPGVQAYAGGWSGDNIASWDHLRHNIRLGISVMISGQAMFGHDI
                     GGFINHPSNEMMTRWVQWGVFNPFARAHSMDVEGEPDRPPWAFDSPYFEIIREHIRFR
                     YELMPYLYTLAHRSTTNGTPMNTPPAFHFRADGNMYATNSQPNEYDFMVGDSLLVAPV
                     WTEGARQREAYLPYGTWWYDYRYDTRHAGGQTLAVPASVASIPLFVREGSIIPMGPWM
                     NYTDESAPGYLNLHVWPAASAEFTLYEDDGISTNYLAGQYASARLTSQTNAASFSFTV
                     GARQGSFDPGERDYYIVAHDVPPASGVTLGGSAVTRVAPAAIRTNTGAAWCYDAASRR
                     LTVKVPDTGGERAVVANYRGSETAAPAAYTSSHSNLAVAGTFNNWNEGARNMRKVTNN
                     TWAAVLELPATNRVEFKFVANDSWSTNWGDLNQGGQQVPISGTADSSALNIVATNLAA
                     GLYTFRFNDQTLAYSVQTAASSDSDGDGMDDEWETDYGFQPLNRNDAAGDESGSGLNN
                     LGKYIAGLDPLSPTATFEVDGLFPAGAGGFRLAWDAVSGRFYGIWFTTNDLRMPSWAE
                     VPGLTNLVGNGPTNALDTNTSSDLRFYRIGVRRQ"
     gene            18676..19155
                     /locus_tag="PK634_06855"
     CDS             18676..19155
                     /locus_tag="PK634_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOW97492.1"
                     /translation="MKIVLTGAIGAGKSTVVREVMSRLGWREPAGFCTRRQRDEVIVE
                     TWRGERRVCARRQDGRAPPYEVMPGAFADFAVKCLGAGREPVVLDELGLLELEVMEFT
                     GAVADLFRRDGPVLAVIQERALQRWREIIGAANIRRVCRVEAENRDRLPDEICARLR"
     gene            complement(19171..21513)
                     /locus_tag="PK634_06860"
     CDS             complement(19171..21513)
                     /locus_tag="PK634_06860"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW97493.1"
                     /translation="MRERFLIGMWLILLAAAAAQEPLGVTSGPGWTTFRVWAPNARSV
                     EVVGDFNGWKASAAERLALDKASGIWSGTLQRSLPSGAYRYLINGNLPRRDPYARAVT
                     PDGRSSAFYDPAAFAWQDDRPAARPIEDLVIYEMHIGAFYDPKPQDGLPGTFQDAIKA
                     LGHLVELGVSAVCVMPVHEFNGLHSWGYNPSDVFAVEQAYGGPDGLKAFVKACHKAGI
                     AVHLDVVHNHYGPDNLDLLRFDGPGGEREGGIYFYDQPGLASTPWGPRPRFDEPMVRR
                     FIKDNTMMWLEEYRVDGFRWDSPINIRAWREGAEPIPAGSQMLEDINSQIRSRFPQAH
                     SIAEDSMDIGNFHGSWDFEFHHQVLPVLKAATDAERGIGPIGSALEWRPSTMARVIYV
                     DNHDEAGQINRQTRIASDVDPANPAGERARRLCALGAVLTLTAPGVPLILMGNEFQES
                     GPFHDNRPLDWGKKQRHAGQFALHRDLIRLRRNLDGRGEALKGMEIRVPILDSERQWA
                     VYWRWHERAPGAPMVAAVNFSEQPVENAEIPFPAPGPWDMLVDTERAQYGGPVRQEIV
                     RAILADKPAGKVRMTLPPFSARLFALAPGARAATSPSEAPEETGATRKQAASLYTAMR
                     LISNVNNWDPKGWPMKLVKDFEWEGRAEFRQAENVELKLAADDGFLYWGAADDWAVPV
                     PHTGTLKRRGDGVKLEGALDGAYRVRFNEDTLEFRIEPAGAGEAPGEFRTWTGASGSR
                     VQARLVSVKGGQVILERPDGQRVSITIDGLSAADQEYLQR"
     gene            21725..>22242
                     /locus_tag="PK634_06865"
     CDS             21725..>22242
                     /locus_tag="PK634_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOW97494.1"
                     /translation="MNTFRSRIAAAGAAVLAASAPCALSESSRFDLGEITPAGPTPQV
                     LLGAADRTDGGAAELRGAADLANAAALLPGVSPDRDGDFNAKTIRVRGFGLNQAPLFL
                     DGLPAGPPPGGRADLGRLGLLDMADISVARGFSPLAYGPNTLAGAVNAVSRKPARSRE
                     LLLRGGAFSGEGL"
BASE COUNT         3358 a         7750 c         7603 g         3531 t
ORIGIN      
        1 ggacgcgatc gcgctccggg cactccagcg gcttaaggag cgggggctga tcgaaaaggc
       61 ggagatcgag ccgttctatg tcgaactgac cggcatcgcg aggcgctacc tggaaagccg
      121 cttcggcctg cgggcccccg agcggaccac cgaggaattc atccgcgagg cgtcggtctc
      181 gggcctgctt tccgcggcgc accaggcgct ggtccgggat ttcctggagc agagcgacct
      241 cgtcaagttc gcccgtttcg cgccgggtat tccggagatg aacgccgcct tcgcgtcggc
      301 tgaaaagctg gtcgtggaaa cccgccccgc cccggcgacg cagggaggcc cggcatgagc
      361 ctccagcacc cgtggtttct cctgctgctc ctgctcgtgc cgggactcgt ctggtggcgg
      421 catggacgcg cgcggcggcc caccctgcag ttcagcgacg gccgggcgct ggggcgcctt
      481 cccgtttcct gggcggtgcg cgcccgctgg gccctgcccg tcctgtacgc cctcgccctg
      541 ttctgcctgg tcgtcgccct cgcccgcccg cgccgggggc tggccgagag ccgcgtgcgg
      601 accgaaggcg tggacatcgt gctgctctcg gacgtctcga cgtccatgcg cgccgaggac
      661 ctctccaccg ccacgcgcac gatgaaccgg ctcgacgccg ccaaggcggt catggaggcg
      721 ttcatccagg cgcggccgga cgaccggatc gggatcgtcg ccttcgccgc catgccgtac
      781 accctcgcgc cgctgacgct ggaccacggc tggctggtgc agcagatggg acggctggag
      841 acgggcatgg tggaggacgg cacggccatc ggcgacgcca ttgccgccgc gatcaaccgg
      901 ctacgcgaca gcaaggccag gagcaaggtc gtcgtgctgc tgaccgacgg catgaacaac
      961 cgcggcgtgt tgagcccgga gaacgccgcc gaggcggcgc gcgccatggg catcaaggtc
     1021 tacaccgtcg gcgccggcgg cgacgaaccc gcccccgtgc cgatctccag ccccttcggc
     1081 ggacggcagt atgccttcca gccctcggac atcgacgagc ccatgctgaa atacgtcgcg
     1141 gaaacgacag gcggcctgta cttccgggcc cggaatttca aggggctgga cgacgtgtac
     1201 cggcagatcg acgagatgga gaaaaccgag gtggacgtcg agcactacac gcggttcgag
     1261 gagcggttca tgccgtgggt catcgcggcc ctggtcctgc tggggctgga gcggctcctc
     1321 gggctcgcga ggctggaggc ggcgccgtga tgaaatgggg agcgccagag ctgctgtttt
     1381 tgctcgccct gctcctgccc gcggcgtggc tggtcttctt cctgctccgc cgccgcgagc
     1441 ggcggctggc ccggctggtc gacggctcgc agttcgccct gctggcgccc ggccggaacc
     1501 cgcagcgcgt gcggacgcgg cacctcgcct ggctcggggc cctggccctg atgctggcgg
     1561 ccctggcgcg gccccagtgg ggcacgcgct gggaggaagt ccgccggcga ggcctcgacg
     1621 tgctggtggt gctggacacg tcgaagagca tgctggccga ggacatcaag cccaaccgcc
     1681 tgcaacaggc caagtggggc atccgcgacc tcctccggca actgcgcggc gaccgcgtgg
     1741 gcctcgtcgc gttcgccgga agcgccttcc tgcaatgccc gctgacgatc gactaccccg
     1801 ccttcctcat gacgctggac gacgtgtacg tcgggctcat cccccgcggc ggcacggcca
     1861 tcgcccaggc cctgcgcacc gcgatggaca gcttcgagga ggatgccggc tccgaccgcg
     1921 ccctcatcct catcacggac ggcgaggatc acgaggggaa gcccctcgcg atggttcccg
     1981 agttgaagaa gcgcgggatc cgcgttttcg cggtcggcgt gggcacgctg gagggtgaac
     2041 tgatccccgc gcccgacggc agcgggtcgt cggcgtttct caaggaccgc gagaatcgcg
     2101 tggtgaaatc cagcctgaac gaggacctgc tggcacggtt ggcggtggag accggcggca
     2161 tgtacgtgcg ctccacggcg gaggatttcg gcttggaccg gatttacgag aaggggctct
     2221 cggaactccg ccgcgacgag cacgagggcc gcatggtccg gatgcacgag gaccggttcc
     2281 cctgggcgct tggcggggcc ttcatcctgc tggcggcgga gagcctgctc cgcgagcggc
     2341 cgagacgggg aaaggagcgg ccggcatgaa gaatatcctc ctggcctccc tgcttctctc
     2401 cgcgctgcgc gccggcgcgg ccgacgatcc ccgggcgcag ctgcgcgccg ggttgaggga
     2461 atacgagcag aagcagtacg agtccgccct gcagcagttc gagggcgcgg cggcgggcgc
     2521 ggcgaccaac gagctggacg ccgccgttcc ggcatacaac gccgccaacg cgctcctgca
     2581 gctcgaccgc gccgacgagg cctcgcagcg gtaccaggac gccctgcgct ccccggacct
     2641 cggactccag ggccgggcct tctacaaccg ggggaactcg ctctaccggc tcgcggaggc
     2701 ggcggcctcc gcccccggcc cggccatgcc gctgcccgcc gcgcccccgg ccgcaacgac
     2761 cccgccgacg gcgccccagg cccccggcct ggacacggcc ggccaggccg tcgaggaatc
     2821 actcaagatg tacgagcgcg cgatgttcct ggatccggcc gacgaagacg ccaaggccaa
     2881 ttacgaactg gcgctgcagt tgaaggaaaa gatccagcag atgcagcaac aacaacagca
     2941 gaagcagcaa aaagaccagc cgcagcagca ggataagaaa gagcaggagc agaaggagaa
     3001 ggagcaggag caagagcaag agcaggaaca agagcaagag caggagcaag agaagaagca
     3061 ggggcaagag cagaagcagg agcaggccgc gaagccggaa ccgacgccgg ccgagcagat
     3121 gacgcccgag gaggcgcgcc tgctgcttga caggatgaag caggaagagc agtcgtaccg
     3181 ggacaagttg caggttatca tggggcgccc ggtgcccgtg gataaggatt ggtagaaggc
     3241 atggctcgac ggggatccat tcgagcggcg ttgatcggcg gcgcggccct ggccgcggcg
     3301 gccgggcagg cagccttcgc tgtcacagcg gatatggatg tcaacccgcg cgctgtgtcc
     3361 gtcggcgaat ccgcgatctg caacatgacc gtccacggcc tggacaatcc cccgcccccc
     3421 tcgctgcccg ccgtcccggg cctgcagatc agccccgtcg gcacggagcg gagctggtcc
     3481 atgggtcccg gcggccagga tagctccgtg agcttccgct tccgggtgct gccgctgcag
     3541 gccggcaagt tctccatcgg gccgatcgcc tacgaggccg agggtcaacg gatggacatc
     3601 cccgcagtcg agctggaggc ggtggaccag ggagcggcgg cgacctcgga cggcggcggt
     3661 tcgccgatcg ccgacctgct cttcgcgacg atctccgcgc agccgacgaa cgtgtacatc
     3721 cagcaggtgt tcgacgtggt cctttgcatc tactcgcgca acatcaccct cgggcgggat
     3781 gtcgagctgg gcaacatgcc ggcctcggga ttgagcctgc cccccttcca ggagatgggt
     3841 tcgacccgcg aggtggtccg caaccagatc tacgacgtgc gccggttccg ctgcaaggcg
     3901 caggccctga cggcgggcgc gttcacgctt gagccgaata tccgcgtgca ggtcgtggtg
     3961 ccgcgcgacc gccgccggat gtcgccgttc gacgacttgt tcaacgatcc gttcggcaat
     4021 ccgttcatgg gccgctacca gacgcaggcc gtggacctgc gggcggagcc cgtggtcctg
     4081 aacgtgcaac ccctgccccc cgccgacggc gtgcccgggt tcgccggggc cgtcggccgc
     4141 tttgcgttcg atgtccaggt gaagccgacc gaggtcagcg ccggcgagcc ggtcacgttg
     4201 tccatgcaga tcggcggcgt gggcaacatc gagaacatct cgccgcccgc gatcgccgcc
     4261 ggcccggatt tccgcctgta cgaaccgcgg caggtgtccc gggacatcga cggcgcccgc
     4321 tccggcgggc gggtgctcta cgagcaggtc gtcatcccgc ggtcggacac caacgcccag
     4381 ctccccgcgc tctccttttc gtatttcgac ccggagctcg gccggtacga gactgtcacg
     4441 cgcgggccgt tcgacctggt cgtccacccc gcgaccaacg gcgcggggcg cgtcatccag
     4501 gcggcggccg atgtccccga ggcgcgcacg atgatcctcg gcagcgacat cgtctacctc
     4561 aaaccgccgc cgccccgctg gccccggccg ggaaacgcac cgtcaatccg cgcggtcttc
     4621 tggggcgtcc aggccgtgcc ggcgctggcg gtgctcggcc tgttcctggc cgcgcggcgg
     4681 cgcgagcaga tgagccggga cgtcgcgcgc gcgcgccggg cccgcgcgcc gcgcagcgcc
     4741 cggcccgccc tgcaccgcgc cgaaaaggcc ctggccgggg atcgccgcgg cgaattcgtg
     4801 gacgccctgc aggaggccct ggccgcctac ctcgggaacc ggctcaacct ccccgcgggc
     4861 gcggtcacgc cggatgtgat catggcccgg ctcgagaagt cggcgctgcc ggccgacggg
     4921 atcgcccgcg cgcgggatct cctggcgttc tgcgagcggg agcgatacgg acagggttcg
     4981 ggccatgtgg aaggcgaccg ggcgctgctc gcggcgcgcc tcgagggatt acgcggcatt
     5041 ctcaaggcct gcgaaagggt gcgtctctga tggacatccg cgctccatgc cggatcgcgc
     5101 tggccgcgtt gctggccttc gccccgcgcc ctgccgcggc ccaaccctcc gacgaatggc
     5161 cggccgccaa ggcgcagcag atgttcctcg aagccggcca ggcctatgac caggggcggc
     5221 tggcggacgc ggttgaagcc tacgagacgc tgcgcgggcg cgggctgcgg ttccaggagc
     5281 tcttcttcaa cctcggcaac gcctacttcc ggcagggcga tctcggccgc gccatcgtca
     5341 attaccgccg cgcctgggtg ttgaacccgg gcgacccgga cacccgggcc aacctgcgct
     5401 acgcgctgca gaccgccggc gttcccgaac cggccctgcg cgggacccag cgcttcctct
     5461 tccgcgcaag ccgggaaacc tggcgttgga tcgccgtggg cgcatggtgg ctcgccgccg
     5521 gcctggcggg cgcctacctg tggtcgcgtt cacggccggc ctggatggcg aagctggcgg
     5581 tggcgctgct cgcggtgatg ctcgtggcgg cctggggagc gctcgcctgg gaccgcttgt
     5641 gccgcgcgcc ggagatcgtc gtggagggaa agggccacga agcgctgttc gccccgctgg
     5701 ccgggtccac cgctcatttc gcgctgccgg aaggaagcat cggccgggtt gtggagcatt
     5761 ccgggaactg gatccggatc gccagcggcg ggcaggaagg ctggatccag gccgagcacg
     5821 gcctgcccgt atgctattgg cttgtcgaga acagcctctt cagtcatgat tgaatgaacg
     5881 aggacgctcg attcatgagc acgccgcatc caaagccgcg atacaataag ggttttttct
     5941 ggaccgcctt ggtgttgttg ttcatcggcg ggaccatggc gttgcttctg tcggtggacc
     6001 ggaccaacat ctcggcgcgg ttggccgcgc gcgcgaccat cctgctcacg ggttccgcga
     6061 tggcgctgtg cctcatctgc ggcaccgccg actggtggct gaaacggtag gcgcggccgc
     6121 gcccctcatt ccacgtagcg ccggtcgagg gccgcgttga tgtccgtcag gatctcgtag
     6181 gggatggtgc ggcaggcggc cgccagttcg ccggcccaga tggattcgga tcgctgctct
     6241 ccgatgagca ccgcttcgtc cccctcctgc gccgtgcggt cgtgaccgag atcgacggtg
     6301 atccagttca tgctcacgcg gccgacaacc gggcagcgcc agccgccgat gaggacgacg
     6361 cccttgttcc ccagcaggcg gttgtagccg tcggcatagc cgacgctcaa cgtggccagc
     6421 gcggtcgggg cggtggtcgt gaactggctg tagtacccca ccgagaaccc ggccggcacc
     6481 cgcttgacct gcatgacgtg ggccttccat tgcaggatcg gcttcgtgtg aaagcgcagg
     6541 gcgggatccg cggccccgta gccgtagagg acgatgccgg gccgcacggc gtccacgtcc
     6601 cagtcgcgaa aatagagcgc ggcgcggctg ctcgacacgt gccggaaaag gcggccgccg
     6661 acgcgctgtt ccacggcggc cgaggccgcg gcgaaccgct cccactggac gcgggccggg
     6721 gccggcttgc ggggctccac cgtggcgaag tgcgtgcaga gcccttcgat ctcgaggccg
     6781 catggggcgc ccaggcggtc cagcacggcg ccggcgtcct cccaccagat cccgagccgg
     6841 cccatcccgg tgtccacctt cacatgcacc ggcagggtct tgcccatgga cttcgcggcc
     6901 tgcccgagtt ccaccgcatg cttttcgctg atcacgacgg gggtgatgcg ccggccgacc
     6961 agcagcggca catcggacgg ctcggtcgcc cccagcacga gaatccggac gtccggcagg
     7021 acctggcgca cctgcagcgc ttcgtgcagg tacgccacgc cgaaccagcg gaccccgtgg
     7081 cgggccgcat gcacggccac cggcaccagg ccatgcccgt aggcattggc cttcacgacg
     7141 aacaggattt ccgtcggagg gcgcaaggcc agccgcacgg cgcggatatt ctccgccagc
     7201 cttttcaaat cgacctggac ccacgatgtt ttcatcggaa acgcgcccat tatgcctatt
     7261 gtctcccaag ctgcaagggg atcgtcaccg cctcggcgga ttgacatgcc gcggcaccgc
     7321 ccgtaccttc gcagcacaaa tgggcgcccg catccaatcc gtctctaccg acggacgcgc
     7381 cgtcgaacag gcggccgcct gcctccgggc cggaggactg gtcgtgttcc ccaccgaaac
     7441 cgtctacggc ctcggcgccg atgcgctgaa cccgaaggcc gtggcccgcg tattcgaggt
     7501 caaacagcgc ccccgtttcg acccgctgat cgtccatatc gccgacatcg agtggcttcg
     7561 cgatatcgcc gcggcgttcc cccccgcagc ccggaaactt gccgaccgct tctggcccgg
     7621 cccgctgacc ttcgtcctgc cgaaaacgga ccgcgtcccc gacctggtca cggcgggcct
     7681 gccttccgtg gccgtccgca tgccgcgcca tccgctggcc ctggacctga tccgccaggt
     7741 cggcggcccg gtggccgcgc ccagcgccaa ccggttcggg cacttgagcc cttccaccgc
     7801 ggaggacgcc cggacggaag tcggggaggg cgcggacatg atcctcgacg gcgggccctg
     7861 cccggtcggc atcgaatcca cgatcgtttc ccttctggat cccaatcccc tcctcctgcg
     7921 acccggcggg ctgccgctcg aggacatcga atcggtcatc ggcccgctcg cgagagcgcg
     7981 gcatgcggac gagcggccgc aaagccccgg ccgcctgctt cggcactacg cgccccgcac
     8041 gccgctttcg ttggtgtccg ccgtgccgcc ccgggccgac cgggccgggg ccggcctgct
     8101 ttcccccgga cccgtcccgg aggccggcgg cttcgccatg gtcgaaatgc tctccccggc
     8161 gctggatgcc gccgaggccg cggcgcggct cttcagcgcg ctgcgacgcc tggatgccgc
     8221 ggggttgtcc cgcatctatg cgctcgaggg ccccgagcgc ggactctggc gcgcgatcaa
     8281 cgaccggctc cgccgggcct ccgtgcgcga cgccccggac gaaatccccg gatgaagaag
     8341 gccctgaacc tgggcgccgc cctggccgtg ctcgtgtgcc tgggcggcat cttcgcctgg
     8401 agcgagttcg ttccggccct ccggaaggcc tatggataca ctgcggccca gacccaatgg
     8461 atcttcgggc tcacgatcgc ctcgttcacg accagcatga tcggggccgg ccgattgctg
     8521 taccgctggg gccccgcccg ggtggccgcg acgggcgggt tgctgttcgc cgccgggtat
     8581 ctcgtcgggg ccttcacctg cgaccgcttc gccggcttgc tcgcgggcct gggcctcctg
     8641 gccggatgcg ggatcgggtt cggctacgtg gcgccgctgg ccacggtcat ggcctggtcg
     8701 ccgggccgtc ggggactggc gggcggcatc gcggtcgccg ggttcggaag cggcgcgatc
     8761 ctcctggcct ccctggcggc cgcgggatgg cgccacgggc ttgaggtatc ccggctcttc
     8821 gggatcatcg gcctgtcgta cggagccgtg atcctgctgg cggcagccgg tctttcccgg
     8881 ccgccggact ccgtcgtttc tgccggcgcc gggcgggccc ggccctggaa ggacgcgcgc
     8941 ctccgacgat tgtttctcgg catgttctgc ggcaccttcg ccgggttgat catggtcggc
     9001 aacctcaaac ccatcgcgct ctccgggggc atcgcggcgg gcgccgccac ggcctccatc
     9061 agcatcctgg ccggcgcagt cctgctgctg ctccccgccc gcgcgagcgg gccccttttc
     9121 ggcctgggcg ccggcctcat cgggctcggg ttcggcgcct gtttcgtggt gtacgcggcg
     9181 gaggtggcgg cccactacgg cgccgggcag gtcgggggcg tctatcccct cgtcttcctg
     9241 gcctacggcc tcgccggact caccggcccc gcgctcggcg gctggctttt cgatgtctcc
     9301 ggcacgtatc gctactccat gctgctggcc gccgcggtcg cggcgtcggg cgcggcgctc
     9361 gccaggatgc ccgccgggcg cggagccccg acggcgccgg catttccgtc ttgtcaccgc
     9421 cctctttttt tcacagattc atccatcgcg gatcgaacga agggaacttg agcatgacgg
     9481 attcgagcca ctttcagccg gcctggaact tcctgggcct gcccgacgaa ctcgcggcgc
     9541 cggagcgcgc ccgggcgtgg atactgccga tcccctacga gggcacgacg tcgtacggcg
     9601 ccggcacccg cgagggcccg tcggccatca tcgcggcgtc gaggcaggtg gagctcttcg
     9661 accgtgaatt cgacggtgag ccggcgatgg cgatcggcat ccacaccatg aatcccctcg
     9721 gcctcgagca tggctcgccg gaggccatgg tggccgcgat cgagaacgcc gtcgcgggca
     9781 tcctgtcggg cacgccgcgg cccgagcagc tcgtcgtgct cggcggcgag cactcgatct
     9841 ccggcggcgt cgcgcgcggg atgaagaagg ccggcctcaa ggattttgtc acggtccacg
     9901 tggacgccca tgccgacctg cgggcggagt acgagggcac gccgtacagc cacgcctgcg
     9961 cggcgcggcg catcgtcgag gtctcgccca tcttccaggt cggcatccgc aacctgtccg
    10021 agggcgagga acgcttccgg cgcgggaaca agcggatccg gaccgtgttt tccgaggagg
    10081 cgaacgcggg ccgcggctgg ctgaaggagc tggcgaagtt cgtgaaagga aagcaggtgt
    10141 acttcaccat cgatctcgac ggcctcgatc cctcgatcat gcctgccgtc ggcacgccgg
    10201 agcccggcgg gctgtcctgg gaacggctgc tggatgtcgc ccgcgtggtc tgccgcgagg
    10261 cggcggacgt gcccgtgttc gacgtcgtcg aactggcccc gatccccgcc ctgaagggcc
    10321 cggacttcct ggcggccaag ctggcccaca agatcgtcgc gctgtcgctg taccgtcgtc
    10381 ccgcccgcag gcgttgaaac gcaagaggag aaccatgagc aagatcacgc tgccgaaaac
    10441 gttcatcagc aagtaccccc acaagttcgc caaggggaag acccggttcc tgtcgggcaa
    10501 acgcatcctg ccgccgccga tcacggggaa ggagagcgtg ccggagctgg tcaaccgcac
    10561 gttcctcgcg tacaacgccg cgcgcctcca ggagggctgc cggctgttcg cggagaagat
    10621 gctggccaac gacgacgtca cggtcggcat gagcctcgcg ggcgcgctga cccccgcagg
    10681 cctcggcgcg gccgccgtcg tgccgctgat caaggcgggc ttcgtggact ggatcgtcgc
    10741 caccggcgcg aacctttacc acgacatgca ccacgcgatg aacctgccgc tctacaaggg
    10801 caccgccagc atcatggacc ccgtcctgcg ccgcctcggc gtggtccgca tctacgacat
    10861 cttcctcgac tacaacgacg tgctcatgcg cacggacgac atcctgcgca agatactgat
    10921 ccagccggag ttccaaaaag agatgggaac gccggaactg cactacctgc tcggcaagta
    10981 ctgcgccgag ttcgagaagc agacgggcct caaggacgtc tccgtgctcg ctgccgcgta
    11041 ccgcgccggc gtgccgtgct tttgctcctc gccgggcgac tcgaccatcg gcatgaacgt
    11101 cgccggcgtg gagctgcgcg gcaacaagct gcgcgtcaac ccaagcatct gcgtcaacga
    11161 gagtacggcc attgtgctgg acgccaaaca gcgcaggaag aagaccggcg tcgtcctcgt
    11221 cggcggcggc tcgccgaaga acttcacgct ccagacggag ccgcagatcc aggaggtcct
    11281 tcgcatcaaa gaggccggcc acgactattt cctccagttc accgacgccc ggccggacac
    11341 cggcgggctc tccggcgcga caccgtccga ggccgtgagc tggggcaaaa tcgatccgaa
    11401 ccaactgccg gacgccgtcg tgtgctacct ggattccacc gtgggcctac cgatgctgac
    11461 gcattacgcg ctggcgcagc gcaagccgcg caagctgaag cggctctacg acaagcgcgg
    11521 cgcgatcatg gcccgcctga cgaaggagta cttcaagcac aatccggacg cgacgcggat
    11581 cgggtgacgg gagccgcgcc atgccctccc ccgccctgct gaagcagctc gcgaaaaagc
    11641 acgggacgcc tctcttcgtg gtggatcatg acgtgctgcg gcgcaatttc gccgagttcc
    11701 ggaaaggcct gccccgcgtg cagccgtact acgcggtcaa ggcgaacccg gacacggcga
    11761 tcgtgcggac gctgttcgag gcgggcgcga gcttcgacgt ggcctcgatg ccggagttcc
    11821 ggatcgccta cgagaacatc cggaagatgc ccccgaagcc ccgccagcac tggatctggg
    11881 acaagatcat ctatgccaac ccgatcaagg acaacgacac gctccggcag ctgaatccgt
    11941 acaagccgct ggtgacgtac gataacctcg aggaaatccg caaggtccgg aagtacgcgc
    12001 cgcacgcggg gctgatgctg cggctcaacg tgccgaacac gggctcggtg gtcgagctgt
    12061 cgtccaagtt cggggcgtcg gcgggcgagg cggtggatct catcgccgcg gcgttcaagg
    12121 cggggctggt ggtcgagggc gtgagtttcc atgtcggcag ccagtgcgcg aatttccaga
    12181 acttcgtgca ggcgctggcg atcgcggatg gagtgatgaa ggaggcgcgg tcccgcgggt
    12241 acgcgccggt gaagatcctg gacatcggcg gcgggttccc ggcgccctac gaccggcatg
    12301 tgcggccgtt cggggaactg gccgcggtgc tgaacgccga attcgaccgg ctgttcccgc
    12361 cggacatgga gatcctggcc gagcccggcc gattcctcgt cgccacggcg gccacgctgg
    12421 tggtcgagat catcggaaaa tcgcggcggg acgggaagct gtgcttgttc atcaacgacg
    12481 gcgtttacgg cacgctctcc ggcgtcctgt tcgaccactg ccagtaccac ctcaaggcgt
    12541 tccgcaaggg cccgacggaa atctgctccg tgttcggccc cacctgcgac gccctggacg
    12601 tggtgtccgt ggccgaggac ctgccgacca acctgaaact gggcgacctg ctctacagcg
    12661 agaacgttgg cgcgtacacg accgccagcg cgacgcactt caacggcatg cccctggcca
    12721 ggatcgtagc cctgaaccgg tgaggcccgt gaccgggccc gcggaccatg ccgccgtttt
    12781 gcgagttgcg ccgcgccgga ggatggtgct tacttcttcc accctatgga cacgaagccc
    12841 aaacgccggc tcgtctggct gacgcgaacc ttcctgctgc tgatcctcgg actgctggcc
    12901 gcggaagaga cctgccggct cctgcccgcg ccggtctcgc tgccggtgga caaaacgcgc
    12961 atcagcttct gggcggacca ggatagacag catccgtggt tgacgaacgg ctccaacgcg
    13021 ttgcgcgtgg cgatgctggg cgattccatc accgcggggg ccggggtccg gggccaggtc
    13081 atcttcggcg gccagctgga atggctgctc aacctgaacc gcgacacgcc gccggcgatg
    13141 gtgggcgtgt ttgcccaggc gggcacgtcg cccgtcaacc agaagttcct gctcaggaag
    13201 gccctggaca tccagcccag cctgctgatc ctgggcacca cggtcagcga cctggaagac
    13261 cgcaaggtcc cggaacccct gatgagcctg atggcggcca cgcggccccg ggatccgccg
    13321 ccggacatga ccggcctcgc ccggtatagt tgcgcgctgg cccgctgggc tcgggcgcgc
    13381 gagaagcggc gcgtccacga ggcgacccgg cagtattacg attttctgtt ccgggaggat
    13441 tactccggct gggtatcgta ttgctacgcc gtgcgcgcct tccgggacgc ctgccgcgcc
    13501 cgccagatcg agttcatggt cctcgtcctg ccgccgatga acgatgtgcg ggagcatgcg
    13561 gaatccctgg acaagcacca ccgcatgctg cgccgatttc tggccgggga acagatcccc
    13621 tgcctcgacc tgctcccgct gctgcgagag tatgcggcgg agcggctgga actggtgccg
    13681 ggcgtcgact cgcatttcaa cgagctgggc caccgcgtca tcgccgagaa cctgttcgcc
    13741 tacttgctcg ccaacaacct catcccgcag acgtatgtgc cgcagcacat gagccacccc
    13801 tcgctggccc ggctctgggg ggcgcaccaa ccgcgctgtc ccgcgcctta gggcgccttc
    13861 gatccgcgca ccgcccgaca gcaggcgcag gaactcctgg aatacagaaa gtgcgggaat
    13921 cacaaggggc acccccggcc cgactcgaac gggcgaccgt cggattagaa atccgatgct
    13981 ctgtccagct gagctacggg ggtatctcca agcccggcac cacgatagga acgaccggcc
    14041 ggcttttcaa gcccgcaacg cacggcttgc gcgccgcgga acctcatgta cgttatgcgc
    14101 tcacatgagg aaactgggtg cagccttggg catgctcgtg gccgccggct tcttgggcgc
    14161 gcgggccgcc acgcttgaaa tccgggtcac ggggctctcc acctccggcc ccgtgcgcgc
    14221 ggcggtcttt gcatcgcgcg aggcctacga gaaaaaagag ccgctgcggg aaatgaccgc
    14281 ggagcccgat gcggagggcg cgccgttcca ttttgaaggt cttccgcccg gcgagtatgc
    14341 cacccgcgtg ttccaggatc tcaacgacaa cagctcgctc gaccggcacg gatttctgcc
    14401 cgcggagccg ttcggttttt cggggaactt cctgccgcgc ttggggccgc cttccttttc
    14461 cgacctggcg ttccggatcg gcgaggagga tgtgagggaa accatttcac tgatcccgcc
    14521 cccccgccgc ggggaatggt cggcgggcgc cgcgctgatc gcgaagacgg agtcgtaccg
    14581 cggcatggac gaccgggtca tccccctccc cttcgtcagt tacgcaggac cgcggctgca
    14641 ttggttcgga ccggtggcca gcctggccgt gtacgagagc gggggattct ccgtggccgg
    14701 cacggcgcgc tacacgttcg acggctacga gcaggacgat gccccgatct tcgaagacat
    14761 gcctgatcgc gacgccaccc tcgaggccgg ggcgcgggcc ggttgggcgt tcgcgccccg
    14821 atggcgcatc gacacctccg tcgcccacga tctcctgggc cgccacggcg ggttccgggc
    14881 cggcctcggc ctgcgcagaa cgttcaacgc gggggaactg accgccgtcc ccgaggtcgc
    14941 gctcgaatgg atggacgacg ccgtggccgg ctactactac ggcgtgcagc cggcctatgc
    15001 gcgggaagac cggcgcgcct acgcgccgga cgacgcctgg gagccgcgcc tcggcatttc
    15061 gctgctgtac ccggtccggc cgaaatggat tctctccctt cgcctcgccg cccgccttct
    15121 gccggatgag ctgacggaca gcccgctggt cggggacgac gaggagatca cggcgttcat
    15181 gagcgtgagc cgatccctgt aacccggggc cgcggcggcg cgcggccggc tctattgccg
    15241 ccggacgccg atccggtaga agcgcaggtc ggaactggtg ttcgtgtcca gcgcgttggt
    15301 cgggccgttg cccacgagat tcgtcaggcc gggcacctcg gcccaactgg gcatgcgcag
    15361 gtcgttcgtc gtgaaccaga ttccgtaaaa ccgcccgctg acggcatccc acgccaggcg
    15421 gaacccgccg gcgcccgccg ggaacaaccc gtccacttcg aacgtcgccg tcggactcaa
    15481 cgggtcgagg cccgcgatgt acttgccgag attgttcagc ccgctgccgc tctcgtcccc
    15541 ggcggcgtcg ttgcggttga ggggctggaa gccgtagtcg gtctcccact cgtcgtccat
    15601 gccgtcgccg tcgctgtcgg aggacgcggc ggtctgcacc gagtacgcga gggtctggtc
    15661 gttgaagcgg aaggtgtaga gcccggccgc caggttggtg gccacgatat tgagggccga
    15721 ggaatccgcc gtcccggaaa tcggcacctg ctggccgccc tggttcaggt cgccccagtt
    15781 cgtcgaccag gagtcgttgg ccacgaactt gaactccacg cggttcgttg ccggcagttc
    15841 cagcacggcg gcccacgtgt tgttcgtcac cttccgcatg ttccgcgcgc cctcgttcca
    15901 gttgttgaag gtgccggcga cggccaggtt gctgtggctg gacgtgtagg ccgccggcgc
    15961 ggcggtttcc gagccccggt agttcgccac gacggcgcgc tcgccgccgg tgtcgggcac
    16021 tttcacggtc aggcggcggg acgccgcgtc gtagcaccaa gcggcgccgg tgttcgtgcg
    16081 gatggcggcg ggcgccacgc gggtcaccgc cgagcctccg agcgtcacgc cgctcgcggg
    16141 cggcacgtcg tgcgccacga tgtagtagtc gcgctcgccg ggatcaaagg agccctgccg
    16201 cgcgccgacg gtgaaggaga acgaggcggc gttggtctgg ctggtcagcc gcgcgctcgc
    16261 gtactggccg gccaggtagt tggtggagat cccgtcgtcc tcgtacaggg tgaactcggc
    16321 cgaggcggcg ggccagacgt gcaggttcag gtagccgggc gcgctctcat ccgtgtagtt
    16381 catccagggg cccatcggaa tgatggagcc ctcgcggaca aagagcggga tcgacgcgac
    16441 ggaggccggc acggccaggg tctgcccgcc cgcgtgccgg gtgtcgtatc gatagtcata
    16501 ccaccacgtg ccgtacggca ggtaggcctc gcgctgccgg gcgccttccg tccagaccgg
    16561 cgccaccagc agcgagtcgc cgaccatgaa atcgtactcg ttgggctggc tgttggtggc
    16621 gtacatgttg ccgtcggccc ggaagtggaa ggccggcggc gtattcatcg gcgtgccgtt
    16681 ggtcgtggac cggtgcgcca gcgtgtagag gtagggcatc agctcgtagc ggaagcggat
    16741 gtgctcgcgg atgatctcga agtacgggct gtcgaacgcc cagggcgggc ggtccggctc
    16801 gccctcgacg tccatcgagt gcgcccgggc aaacgggttg aacacgcccc actgcaccca
    16861 gcgggtcatc atctcgttgg acgggtggtt gatgaacccg ccgatgtcgt ggccgaacat
    16921 cgcctggccg gagatcatga cgctgatccc cagccggatg ttgtgccgga ggtgatccca
    16981 gctcgcgatg ttgtccccgc tccaccctcc ggcgtaggcc tggaccccgg gccagcccgc
    17041 ccggctcaag acgaaggggc ggccgacggg gtcgcgcgtt ttcagcgtgt cgtaggtcag
    17101 cgaggtctgc cggacgttga acgtgttctt gttgacctgg tgccacttgc ggctgtcgtt
    17161 gttgttggtg cccccgccgt acttgtcgtc gcagtagtac accacgttca gggccatcgc
    17221 gttttcgttg ggctcgttga ggtcgttcca gatgccgggg aagggatact ggtgcaggta
    17281 gttcgtcatc ttcgccttcc accagtcccg cgcaaaagag ctgctgaagt cgagccacga
    17341 gacgttgccc aggaaattcg tgccgacgta ggtggacagg tcgttggcct tcaggaaatg
    17401 caggctggag tacgcctcgc tgtacatcgg gtcgctcgtc ttcagcagcg gctcgatcag
    17461 cgggacgacg cgcacgccct tgctggccgc gtactggatc aggccggcga cgttcgtgta
    17521 gcccgcgttg aacgtcatct ggtggtagtt gtcctgcgtg ccgatgtcga ggtacaccgc
    17581 gtccagcggc acgccgtact gccggaactg gtcgagcagc gtgtgcacct ggtgctgcac
    17641 ccagtaggaa tgccgggaat gatggtagcc gtacgcccac cggggcatct tggcggggta
    17701 gccgatcagc tcggagtaac ggttcagcac gtccagcggc gcatgatcgg ccccgccgcc
    17761 gaagaagtag taatcgagct ggccgtcccc cgcctcgaag gaataggcgt cgccccactg
    17821 cgtgcccatg cggaacgtcg ggcgcgacgg gttgttgaag aacacgccgt acgcccgcgc
    17881 cggcgtcccg ttcgtcgccg ggcgcatccc gtagtacatc gggagcgtca tgtacatcgg
    17941 gttgcgggat tcctgccagt agtacgtgtc ctggttccag ccctggatgt tgtagccgcg
    18001 ccggttcagc ggccccgcga actcgcccag cccgaagtag gcctcgtcgg cgggcatctc
    18061 catgaccgcc ttgagcttga agccgttcgg caggttgctg accgatacgg aaccccagtt
    18121 gaccatctcg tacgagctgt cgctgcccgg ctggtagttc cagtcgtact ccatccgccg
    18181 cgcgcgcagc agttcgtagc cggacgggtc gcgaaacgcg atattgaaca ccggcgtttt
    18241 ctgcacctcg atccgcagct ggctgctcgt gatcacgaac tgcgtgctgc cgctctcgct
    18301 gaaattcacg ctgaacgccg gccagttgga gaacggcttc gcgatgccga cctcctcctt
    18361 gtcccagagc gagtcccagt ggaaccggac gcggaccgcg tccgcggcgt gcggcgcgac
    18421 ttccaccgcg ccggtcgggg aaaggtggaa gatgaaactc cggttgccgg aggccgcgtt
    18481 ggtcacgacc tggaccgacg acaccgggcc gttggtctgg atcgacgccc ggccggaaag
    18541 ggccgacagc gccagcgcga tacacgccgc cttcaccgga tatcgaacga gtgctctcat
    18601 gcccggggcc tccgcctgat tcgtttttac tttacaccca aataaatacg gagtccagta
    18661 tcggtctcgc cacccatgaa aatcgtgctt acaggcgcca tcggagcggg aaaaagcacc
    18721 gtcgtccgcg aggtcatgag ccggttgggc tggcgcgagc cggccggctt ctgcacgcgg
    18781 aggcagcgcg acgaggtgat cgtcgaaacg tggcgcggcg agcgccgcgt ctgcgcccgg
    18841 cggcaggacg ggcgcgcgcc tccctacgaa gtgatgcccg gggccttcgc cgacttcgcg
    18901 gtgaaatgcc tgggcgccgg gcgcgaaccc gtggtcctgg acgaactcgg cctcctggaa
    18961 ctggaggtga tggaattcac cggggccgtg gcggacctct tccgacgcga cgggccggtg
    19021 ctggccgtca tccaggagcg cgccctgcaa cgttggcggg aaatcatcgg ggcggcgaat
    19081 atccgccgcg tctgccgggt cgaagccgaa aacagggacc ggcttccgga cgaaatctgc
    19141 gcgcgcctgc gctgaacatc gccgacggcg tcaacgctga aggtattcct ggtcggccgc
    19201 gctcaagccg tcgatggtga tcgacaccct ctgtccgtcc ggccgttcga ggatcacctg
    19261 gccgcccttg acggaaacca ggcgggcctg cacccggctg ccgctggcgc ctgtccacgt
    19321 ccggaactcg cccggcgcct cccccgcccc ggccggctca atgcggaact ccagcgtgtc
    19381 ctcgttgaag cgcacccggt acgccccgtc gagcgcgccc tcgagcttca cgccgtcgcc
    19441 gcgccgcttg agcgtgccgg tgtgcgggac cggcaccgcc cagtcgtccg ccgcgcccca
    19501 gtagaggaag ccgtcgtccg ccgccagctt cagctcgacg ttctccgcct gcctgaactc
    19561 ggcccggcct tcccactcga agtccttcac cagcttcatc ggccagccct tcggatccca
    19621 gttgttcacg ttgctgatga ggcgcatcgc cgtgtacagg gaggccgcct gcttgcgcgt
    19681 ggcgccggtc tcctcgggcg cctcggacgg agacgtcgcg gcccgggccc ccggcgccag
    19741 cgcgaacagc cgcgcgctga acggcggcag cgtcatgcgg accttgccgg ccggcttgtc
    19801 cgccaggatc gcgcggacga tctcctgccg gacgggcccg ccgtactgcg cgcgctcggt
    19861 gtccacgagc atgtcccagg gccccggcgc ggggaacggg atctcggcgt tctccaccgg
    19921 ctgctccgag aaattgacgg ccgccaccat cggcgcgccg ggcgcgcgct cgtgccagcg
    19981 ccagtacacc gcccactgcc gctcgctgtc gaggatcggg acgcggatct ccatcccctt
    20041 cagcgcctcc ccccgcccgt cgaggttgcg gcgcagccgg atgagatcgc ggtgcagggc
    20101 gaactggccc gcgtggcgct gcttcttgcc ccagtccagc ggccggttgt cgtggaacgg
    20161 cccgctctcc tggaactcgt tgcccatcag gatcagcggc acgcccggcg cggtcagcgt
    20221 gagtactgcg cccagcgcgc agagccggcg cgcgcgttcg cccgccgggt tggccggatc
    20281 gacgtccgag gcgatccgcg tctgccggtt gatctggccc gcctcgtcgt ggttgtccac
    20341 gtagatcacc cgcgccatgg tggacggccg ccactcgagg gcgctcccga tcggcccgat
    20401 gccgcgctcg gcgtcggtgg ccgccttgag cacgggcagg acctggtggt ggaactcgaa
    20461 gtcccacgag ccgtggaaat tgccgatgtc catcgagtcc tcggcgatgc tgtgcgcctg
    20521 cgggaaccgg ctgcggatct ggctgttgat gtcctccagc atctgcgagc ccgcggggat
    20581 tggctccgcc ccttcgcgcc aggcgcggat gttgatcggg gaatcccagc ggaacccgtc
    20641 cacgcggtac tcctccagcc acatcatcgt gttgtccttg atgaaccggc gcaccatggg
    20701 ctcgtcgaac cgcggccgcg gcccccacgg ggtgctggcg agccccggct ggtcgtagaa
    20761 gtagattccg ccttcccgct cgccgcccgg gccgtcgaag cgcagcaggt cgagattgtc
    20821 cgggccgtaa tgattgtgga ccacgtccag gtgcacggcg atcccggcct tgtggcaggc
    20881 cttgacgaac gccttcaggc cgtcgggccc gccgtacgcc tgctcgacgg cgaatacgtc
    20941 gctggggttg tatccccagc tgtgcaagcc gttgaactcg tggacgggca tgacgcacac
    21001 ggcgctgacg ccgagctcca cgaggtggcc gagcgccttg atggcgtcct ggaacgtccc
    21061 gggcaggccg tcctgcggct tggggtcgta gaacgcgccg atgtgcatct cgtagatcac
    21121 gaggtcctcg atcggccggg ccgcgggccg gtcgtcctgc cacgcgaagg cagccgggtc
    21181 gtagaacgcc gaactgcgcc cgtcgggtgt cacggcccgc gcgtacgggt cgcgccgggg
    21241 caggttgccg ttgatcaggt agcggtacgc gccggacggc aggctgcgct ggagcgtgcc
    21301 ggaccagatg cccgaggcct tgtccagggc cagccgctcg gcggccgagg ccttccagcc
    21361 gttgaaatcg ccgacgacct cgaccgagcg cgcgttcggc gcccagaccc ggaacgtggt
    21421 ccaccccggc ccgctggtga cgcccagcgg ctcctgggcc gccgcggcgg cgagcaggat
    21481 caaccacatg ccgatgagga accgttctcg catggcgttc tcccgcttcc tgtacccggc
    21541 cgcgagaccg ggctcaagcc tgtttcttaa caataggtcc cataggacgt atacgaccta
    21601 tattctcttg cctcccccct ttttcgcctc tatgactatc ggccaagagg ctgcgcgcga
    21661 aagcgagccg gcgggcggcc cggaccctgg tgtcggcgcg ggcccatcac gaaggagatc
    21721 ggcgatgaac acattccgca gccggatcgc ggcggcgggc gcggccgtcc tggcggcctc
    21781 cgcgccctgc gcgctgtccg aatcctcccg cttcgacctc ggcgagatca cgcctgccgg
    21841 cccgacgccg caggtccttc tcggcgccgc ggatcgcacg gacggcgggg ccgccgaact
    21901 ccgcggggcc gcggacctcg cgaacgccgc cgccctgctc cccggcgtct ccccggaccg
    21961 cgacggcgac ttcaacgcga aaaccatccg cgtgcgcggc ttcggtttga accaggcccc
    22021 gctgttcctc gacggcctgc cggccggccc gccccccggc ggccgggcgg acctcggccg
    22081 gctcgggctg ctcgacatgg cggatatcag cgtggcgcgc gggttcagcc ccctggccta
    22141 cggccccaac acgctggccg gcgcggtcaa cgcggtcagc cgcaagccgg cgcgctcgcg
    22201 cgaactgctg ctccggggcg gcgcgttcag cggcgagggg ct
//
LOCUS       DAOOTK010000044        21969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1632_length_21969_cov_5.183901, whole genome shotgun sequence.
ACCESSION   DAOOTK010000044 DAOOTK010000000
VERSION     DAOOTK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21969
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_21969_cov_5.183901"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1031
                     /gene="nadC"
                     /locus_tag="PK634_06870"
     CDS             156..1031
                     /gene="nadC"
                     /locus_tag="PK634_06870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOW97495.1"
                     /translation="MMTPLPDLACDPRLAELVRRALEEDLGAGDCTSEALIGAEESAG
                     AAILSRGSCVVAGLPVAAEVFRQVDAGLDCRVKARDGDPVERDQEVLVLWGRARSILA
                     AERVALNFLQRLSGIATLTRQFARKAAPYGVQILDTRKTTPTLRFLEKYAVLCGGGAN
                     HRIGLYDRVLIKDNHRAAWSRRGGNGLAGAVEEARRRFPGLVIEIEVENEDELREALA
                     ARPDWILLDNMKPGRLARCVTLAKGLCKLEASGGITLKTVEAVARTGVDALSVGALTH
                     SAPAADFSLEFGAGA"
     gene            1036..1668
                     /locus_tag="PK634_06875"
     CDS             1036..1668
                     /locus_tag="PK634_06875"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOW97496.1"
                     /translation="MAEHPPLVYTLREYDSVASTNDIAKTEAVQGAPEGLAILARRQE
                     RGRGRQGRVWLSPEGQGVYLSVLLRPAIPAYEANWLGVIGALAVAGALEQAGVGDLTL
                     KWPNDVLARGRKIAGILVEPRIEAETVEFAVLGMGINVMQDRTEWREDLRDKVTSCRL
                     EGVETEPRRLAGLVLESLGRVYAGFRAGRRQPLLREWSLRTGTSTLPVLE"
     gene            1739..1833
                     /locus_tag="PK634_06880"
     tRNA            1739..1833
                     /locus_tag="PK634_06880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1773..1775,aa:Ser,seq:gct)
     gene            1941..2156
                     /locus_tag="PK634_06885"
     CDS             1941..2156
                     /locus_tag="PK634_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97497.1"
                     /translation="MAHRGDVGVRGDRFARRRHPSSAGTAPTNTTDALLLGVVPSAAG
                     PELFWNGRDRRRYRAWGTAGVEDGAWP"
     gene            2263..4293
                     /locus_tag="PK634_06890"
     CDS             2263..4293
                     /locus_tag="PK634_06890"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOW97498.1"
                     /translation="MKSSRTVSTAFAFVLIVSFAIPGLSQVPSLVAYQGRLLNGTNLY
                     NGPAGIVFRVYDHSDTNLAVLWMEATSVVQVTDGFYATFIAPSNGAADWAWLRSVPPS
                     GDAWWLEVEINGSRFQPLERMAATPYAMVAGAVTNGAISGDMIGSQTVQSYNIADGTL
                     QEQDFDSGVVSSRVIANGSILFADVNQNGAAIGQIIQWNGAAWTNANAVGFKSYSENG
                     TFVINPSAVGTDSIAQGYATYAGAHYSVVGGGRDSRIEARATNAVIGGGFGNRVGTNS
                     FASVIGGGWSNRIGNDVQLGAIGGGYQNRMDGLQASSVIGGGNFNHTYTGAHFAVIGG
                     GKENEIWTNSEYAVIGGGYGNDIDPLARYSMIGGGEGNSIKYGTLHGVVDGGWNNSIV
                     YDSDESVIGGGRDNSIFGDTAWSVIAGGYNNSIQASTPQGVISGGGNNRIGEEANNAV
                     IAGGAVNVISNQSQSAVISGGGNHVIRTNASYAVIGGGENNEIAANAHHATIPGGKQG
                     RANHPGVFLWADASSISTFASATSNEAAFRCSGGVRFTSGSGAANQTVAWTPGSASWT
                     FSSDRSLKEGFVPVDGGTVLERVATLPIAEWNYIGYEERHIGPMAQDFQAAFPLRGSS
                     DKALNSLDLDGVSLAAIQGLYELLNKKDAEIADLRRRLESIESRLNADGVGP"
     gene            4533..7622
                     /locus_tag="PK634_06895"
     CDS             4533..7622
                     /locus_tag="PK634_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97499.1"
                     /translation="MFRISFDQPPDPRRVRILLDTDGPVSGEMVTGADYMMEGQVLYR
                     YPPRARGWKWDTVEPTVTLVEGSTLVAFLPVPPLRGAGSWAVEWLSGEWEVENRLPES
                     GMEPCDFASLSEFRWSTPLQPEDIGDLLRAASASPSAARAGNFDSPAWSNAPPDSTNA
                     PAPAPGGIPLQARLVDAVTGQAAELQPDAIASDGQRWRWTGRALDMEWGLIWRQDSPR
                     DVSVSVRVKSDRARCIRLGLGWRMDLEKAIWHDDARSRAALDRSGARVMRPVAVSYGR
                     RGRVSGFPFGVVTRGSDNYMAETDPAEPRAFEIEADPAASFYGLWYDVALSTQASNFP
                     GQAEIRCSFRTFAGEGGEAFRKALELFYQRHPYFPARKAPVCGFGRPWEKPGFPRTVE
                     FACEARDVWTPPGPETGTPAGCMTFLFLEPWSYWLQAPARLARDEARAGKWLKLLTAG
                     TSRDSELASAAQVGAARRTDGRLAWQEVGLPWHTGLFLRVSAQPRLAPSPEAPLNQAM
                     ADWRLYRNVMHSAPVHGLYFEPPPDAAPMDYNPAALGVAVHPATFSTNRLQPGLASDL
                     EVASDLAVWAAAVRASGGYTAGRPSEAQRAFAAPSLDFIAADFKTWPGDDADSARERS
                     ADSLRALAGLKPVALLRSDAQRPLDADELARHLEWCLFRGFLPGFYSRARGADRLYEQ
                     AQSDEAIRGILEKHLPLIRRLAAAGWRPSGPAAVEPDAIWVESFGRARRPAGQVTVRN
                     PTGERVRGKLRLPGEKGPSMVIDPLTGHCHVTADGPGEFPLVLMPHEIGLRDWFALSE
                     AKEEADFLAGWTGGAREAPAALTNIASVRAEMEWGACCNVLVPVPAVRGDRNEIVIEV
                     RSAGAARLRAVRPKVITSRAFRPADGAAVTVAPGQTERLTAVFEDGDLADDPWLEVQW
                     ELERGEEKMTCVRLLKPRVADAFVFDPPGRRIEAKGGQAVIEIPVRSYSNRPRPLALH
                     WEGDHQGGDRAVELPPDSGTVLRFPIRKKRADSGQMLVEVRSGDSVAFQRWFDVVFR"
     gene            7648..9045
                     /locus_tag="PK634_06900"
     CDS             7648..9045
                     /locus_tag="PK634_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOW97500.1"
                     /translation="MMRASRIAWFLRLAVLSGLLGGTLLLLYWNRIGDPRFYRFLETD
                     AGRWMAGTFRWYGAEAPFIPDERDESRMTPEEIATLRRMEERVRAEQPTHELLFTDGQ
                     ALQGRVIEERPQEVVFVRTYGDSAEVTAVIPRRRLESIRRLEPPAPSISYRDVRFQME
                     FPDLNYYRRPPYSLLTDESFFRVEETVEILEVLHEQFVGIWRGLMEEARPDRSIQVLF
                     FSRQDAFRAYQRQHAPRMESSAGFYSPRLDRLVLFNQRGSERVAELRRQVEEEEARHR
                     AGRLAPEVEARLREWRRQTDQDIEQLAENQTRATIRHEGAHQLFYDFGIHSRHGVEGE
                     WLIEGMATYCEHSPIGAADPTRLNSLRQALEEGRLIGFEELVNLRDESGLLSFGVEER
                     IGLAYDQAWGLVHFLMQPERRNAFFGYLRYLRDPGAFREIRKTGPLALLCRFLEMAPD
                     ELWQQWLEYILQKPS"
     gene            9154..11214
                     /locus_tag="PK634_06905"
     CDS             9154..11214
                     /locus_tag="PK634_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOW97501.1"
                     /translation="MPGRREWVVVALALLAACAESRSEAARIVRVGVYQNPPKIFINA
                     EGRPDGLFVDLLETVAAQENWQLDFMLGSWADHLRALEEGRLDLLPDMAYSAERAARF
                     KLSQIAVLSSWLEVFARHGLPLNTLDDLDGRRVAVLDESIQLQVIREQLPDLGIQVEA
                     EILPSHDEVMAAVQEGRADAGIVNRFFDGAAARRYDVMPTAIIFQPTKLVFAAPASSD
                     GQLLDILDKRLARLKKDRSSAYYRILGQWLGESAPAVRYPRWMRNTLIGLLALVVALG
                     LLGLAMRRVIRVYTRDLRDKTRQLEEALHEVAKAHEEAIKQERLHALGRMASGIAHDF
                     NNCLLSILGYSELMLLKPQAGPDDRAEIQHSLEMIRTAAQDAAVIVKRMQAFYRAQQG
                     DTRLEPVDLNAVVRDAVELSRPSWKTQAESAGIRIHVETSLGQIPFVQGSPSELRDML
                     VNLIINAVHAIEREGTLRVSTSARGEWVALEVKDTGCGMTPEVRARCMDPFFTTKGEK
                     GTGMGLAMVHGIVLDYQGKIVIDSEPGKGTTVTILLPISSKAAAPVPAARALPGLGPR
                     RILVVDDDPRVLKTMSAMLRSVGHVVEVVSSAHEALDRMEVGRPDILLTDLSMPDMTG
                     MDLLHEVRRRGWTLPCLILSGTPEVAQKYSDDPIPILRKPISLTDLNRALAEALASPP
                     GSAG"
     gene            11323..11751
                     /gene="fabZ"
                     /locus_tag="PK634_06910"
     CDS             11323..11751
                     /gene="fabZ"
                     /locus_tag="PK634_06910"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOW97502.1"
                     /translation="MPRLENDEIQKVLPHRFPMLLVDRILECDDKERVVGIKNLSINE
                     PFFQGHFPGHPVMPGVLQLEAMAQVGGVLLSRVLNQPGRIAYFMAIDNARFRKIVQPG
                     DQLRIEVTFEKLRMGMAKVHGVCSVDGDTAAEADLMFVYK"
     gene            11759..12532
                     /gene="lpxA"
                     /locus_tag="PK634_06915"
     CDS             11759..12532
                     /gene="lpxA"
                     /locus_tag="PK634_06915"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOW97503.1"
                     /translation="MARIHPTAIVDPGAELGENAEIGPYCVLGPHVRIGDGARLMAHV
                     FVDGWTTLGAGCAIFPFASLGTQTQDLKFKGGAPRVEIGERTTLREYVTVNAATADGD
                     VTRIGDRCHIMAYAHIAHDCRVGNEVIMANCATLAGHILVEDQAIIGGLCGVHQFVRI
                     GRMSIIGGCSKVTQDVPPFMMADGHPLAIHGINSVGLKRRGVGEEAQRQLKNAYRILY
                     REGLSTSQALERLAAAGAEGPEIRELVEFIRHSNRGIVR"
     gene            12533..14452
                     /gene="gspD"
                     /locus_tag="PK634_06920"
     CDS             12533..14452
                     /gene="gspD"
                     /locus_tag="PK634_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOW97504.1"
                     /translation="MKMRILGLAVLLAAAWAVRAAPPSAPIEPRINFSFDQVDVRLLV
                     KLVGDLTGRRFVVDKGVAGTVTVISPAPVPSSEVYPLFVSILEAHGYTVAEKEGLCYV
                     MPLPERGVTGGRVIGPEGSVPAGGLVTRVIRVQYISALELRKILEPLVRGGKSGALAA
                     FGPSNHLLVTDTADSVKQIERVVAELDRPGAGETAEFIPLEHASAEEVAAQLMTAMRS
                     LSSSGATLSRHVQQVAEGGASLPAEVAVIPAASANGLVLVGAPVQIAELKRIVAMMDV
                     EPVAGKGRLHAVFLKYLSADEAGKSLNALLAKTAEKDQRQRIAIEPNISNNALIVEAS
                     AQDFELVKALLAELDQVPQQVLVEVLIAEVALNKNLDLGVEWAIIESPKDGSTTPVSR
                     SRPGDSDAVMDMITEGVFPQGLSVGVARGTYTDLAGNEVPNIPILIKALAQNRDVRIL
                     SNIPLWAQNNTEASLSVVENIPVLKSTIEGGSGTARDVIQNIDRMDVGIKLKLTPHVN
                     PDREVLMQLNPSIEAIVDEGPEDKFAPTIAKREVSTTVTVPDRATVIISGLIREDEMK
                     AVGKVPLLGDLPLVGWLFRSQSTRKVRTNLLIFVTPHVVTDVREAAAMKRDLERQAQL
                     INAQTNLNQRPARRK"
     gene            14456..16156
                     /locus_tag="PK634_06925"
     CDS             14456..16156
                     /locus_tag="PK634_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOW97505.1"
                     /translation="MRLDEVLVGRGRVTPEQVEEARARAGKAGGDVELGRALVDAGLL
                     ADADLLDALGELYGCDVLREPADNLLDPELVAQLPVEWARAHAMLPVRQGARVVVLTS
                     DPSDLGALDDLALILGTEPAPLLASRREILAAIEHCYVRKTDSPGDLLRGMKPAAGDT
                     PERPADDLLRTSEQAPVTQLINLILLEALKARASDVHLEPYASHLRLRYRIDGMLYEQ
                     SSPPKHLEAPLVSRLKVMARLDIAEKRLPQDGTARVRVGEQEVDIRVSTIPVAEGERV
                     VLRLLNRESTVLPLSKLGMPDSLLARFRELVAQPNGVILVTGPTGSGKTTTLYAALQE
                     LDKAHANILTIEDPIEYQLPHIGQIQVKPKIGLTFASGLRHILRQDPDVILVGEIRDV
                     ETAEIAVRASLTGHLVFSTLHTNDAVSAVIRMADMGVESYLLAAALRAALAQRLVRRL
                     CPHCRRPAVMTDEQARALGSRGGEWVGSPVWQPGECSRCRGGYHGRLGVFELMLVDSA
                     VQEAVRAGRPLGEIRELAARQGMRALHDDALDKIRSGETSVAEVLRAVGRHDVRGAAG
                     "
     gene            16153..17358
                     /locus_tag="PK634_06930"
     CDS             16153..17358
                     /locus_tag="PK634_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOW97506.1"
                     /translation="MKTFAYQGFDHSGRSSAGLVEALDAKEAREKLFARGILVEGLRP
                     AGQGKAGRFRRTGAFDASARAMVYRELAALLKAGLPLTQALEIILSAPETGAHQMLLA
                     DTRDRLREGSSFAEALAAHRAVTPFEKSVVQVGERTGTLDVVLDRIARFLEEESQVRE
                     RIQTALLYPVIVLIMAIAIGMLTMGVMLPRMARLLEEANMDLPAITRFTLALGRWSAP
                     VLLLLIVALGLAGGWLVRRMRAAPEVRQLWNRRAFRWPLAGRMYTALVNLRFARTLAL
                     LLDGGVPLVEALPLAGRATGSSWTGRLAAEQAEAMRHGRNLADALRQIPPLAGSLPVW
                     IQAGEAGGKLSVLLEHAADRYQQQWSRVIARSLGLLEPLLIVLVGGFVLLVALATLLP
                     ILSLNQTLH"
     gene            17368..17772
                     /locus_tag="PK634_06935"
     CDS             17368..17772
                     /locus_tag="PK634_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97507.1"
                     /translation="MRPFNALLSIGLTGTFLFGSTLLAGLVQLHRGDPGHNWTHRDMR
                     LSLAETRNRTEIYIGGELLQDHLGRKGLTATGPDGTVYPVVERDIRIRLNNWPERRAE
                     LAMRAILEAFLTGLSVAALMIGIVQARRRNQP"
     gene            17921..18073
                     /locus_tag="PK634_06940"
     CDS             17921..18073
                     /locus_tag="PK634_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97508.1"
                     /translation="MLEISLPGLSMHIDLLNSWQLSDSIWFGLGVPLIPLRVERCEPT
                     GISQPG"
     gene            complement(18059..18778)
                     /locus_tag="PK634_06945"
     CDS             complement(18059..18778)
                     /locus_tag="PK634_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOW97509.1"
                     /translation="MMQAVILAGGRGTRLSAVWADRPKALVPVAGRPFLEWQLDWLAR
                     GGVRRVHIAGGHMADRIEAWLDSPEAPRALSLTISREPAPLGTGGGLKYAEPGIASDP
                     FLALNGDSLLPGLDFQSLGKAWRSISNDWKQPPAMLAVTRIEEAGRYGTVEFDEGKRV
                     TAFREKAKRESGWVNGGVYVIPRRLLAQLEPGRNLSLETDVFPSLAAQGLLAVFPAPP
                     PLLDMGTPEGIAAMEQYLARL"
     gene            complement(18775..20325)
                     /locus_tag="PK634_06950"
     CDS             complement(18775..20325)
                     /locus_tag="PK634_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOW97510.1"
                     /translation="MNAAPATPREDARRMREVTLLYHVSRVLDQSLDMRDVVTPVLEA
                     LSEYMDMKYATLTLLNRKTGDILIEAAHGLSPQQARRGRYKLGEGVTGQVVLTGKPAI
                     IPKTSESPLFLDKTRRGKRADTSFLCVPIKVEQEVVGALSVDLPYRPSANLEDDVRLL
                     TIIASMIAQAVKLRRSAQEEQERLEIENQRLREELRSRFRPSNIIGNSHEMQIVYDQI
                     AQVAKTPTTVLIEGETGTGKELVAAAIHYNSERADKPFVKAHCAALPENLIESELFGH
                     VKGAFTGALADRKGRFELAHGGTLFLDEIGDIPLSIQIKLLRVVQEREFERIGGTATI
                     KTNCRLITATNKDLPAMVAAGKFREDLFYRLHVFPIYVPPLRKRKADIVLLADHFLEK
                     YSKENNKKVRRLSSAVIDMLMSYHWPGNVRELENCIERAVLVAEGDVIHPYHLPPTLQ
                     TAEAVGTAPSGALKGLVESYERDLIRDALKSSRGNVAAAARTLESTQRILGYKIMKYK
                     IKPKQYVS"
     gene            complement(20322..>21969)
                     /locus_tag="PK634_06955"
     CDS             complement(20322..>21969)
                     /locus_tag="PK634_06955"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOW97511.1"
                     /translation="VSREAAAGGASLVVFPELSLSGYPPEDLVLKRHFLEDCEAQIRR
                     LAGELPPETVVIAGCPRAAGEKAANTAVVFHGGREAACYRKMVLPNYGVFDEKRVFEA
                     GDRAFFLDLDGLRIGVHICEDSWDLDEAAARLLRDQGLDALVNLSASPYHRGKLALRE
                     NVLREAARQLGCPVLYANLVGGQDELVFDGASLALSRDGVLLARARQFEEDVLLVTVA
                     GEVGRRGPPPAPSQVFSLGIRSSVAPRQASTSAARVEPALEDPAEVYAALKLGLRDYV
                     DKNGFGKVVVALSGGIDSALVATLASDALGPDRVKGVTMPSQYSSAETLGDAGLLARN
                     LGIELFTLPIRRLYETYVEELAPAFGARPPDVTEENLQARIRGNLVMALSNKFGWLVL
                     TTGNKSELATGYCTLYGDMVGGFAVIKDVPKTLVFELARWRNRPPGKAPIPPSTIGRA
                     PSAELRPNQKDADSLPPYDLLDAILERYVELDESVPAIVAAGFEPATARRVARLVDRS
                     EYKRRQGAPGIKITPKAFGRDRRMPITNLYDERAEGERLP"
BASE COUNT         3444 a         7276 c         7721 g         3528 t
ORIGIN      
        1 ggcgctgacg aaatacgtca ttggccgaag acgaaaggga tctcggagag gaaccatgaa
       61 acgcacgaaa ccatagaatt cccatcatgt tttcttttcg tgtcttcagt gtatttcgtg
      121 atatccaccg tctttcagga tgttagtctc tgatcatgat gacccctttg ccagacctgg
      181 cttgtgatcc gcggctggcg gaactcgtcc gccgcgcgtt ggaggaagac ctcggcgcgg
      241 gcgactgcac gtcggaggcc ctgatcggcg cggaggaatc ggccggtgcg gctatcctgt
      301 cccgcggctc gtgcgtggtc gcgggccttc cggtggccgc ggaggtcttc cggcaggtgg
      361 atgccgggct ggattgccgc gtgaaggcgc gggacggcga cccggtcgag agggaccagg
      421 aggtgctcgt cctgtggggc cgcgcccggt ccatcctggc cgccgagcgc gtggcgctca
      481 attttctgca gcgtctgtcc ggcatcgcga cgctgacccg gcagttcgcc cgcaaggcgg
      541 cgccgtacgg cgtccagata ctggacacgc gcaagacgac gccgaccttg cgtttcctgg
      601 agaaatacgc cgtgttgtgc ggcggcggag ctaatcatcg catcgggctg tacgaccggg
      661 tgctgatcaa ggacaaccat cgcgcggcgt ggtcgcggcg cggcggcaac ggactggcgg
      721 gggccgtgga ggaggcgcgc cgccgttttc ccggcctcgt gatcgagatc gaggtggaga
      781 acgaggacga attgcgcgag gcgctggcgg cccggccgga ttggatcctg ctggacaaca
      841 tgaagccggg ccggctcgcc cgctgcgtga ccctggccaa gggcctgtgc aaactcgagg
      901 cttccggcgg catcacgctg aagaccgtcg aggccgtcgc gcgcacgggc gtcgacgcgc
      961 tctccgtcgg ggcgctgacg cattccgcgc cggccgcgga tttctcgctc gaattcggag
     1021 ccggagcgta gcgcgatggc cgaacatccg ccgctggtct ataccctccg ggaatacgat
     1081 tccgtcgcgt cgaccaacga catcgcgaag accgaggccg tgcagggggc cccggaggga
     1141 ctggctattc tcgcccgacg gcaggagagg gggcgcgggc ggcagggccg ggtctggctc
     1201 tcgccggagg ggcagggggt ttatctttcc gtcctgctgc gccccgccat tcccgcgtac
     1261 gaggccaact ggctgggcgt gatcggggcc ctggctgtcg ccggcgcgct ggagcaggcc
     1321 ggggtaggcg acctcacgtt aaaatggccc aacgacgtgc tcgcgcgggg ccgcaagatc
     1381 gcgggcatcc tcgtggaacc ccggattgag gccgagacgg tcgagtttgc cgtgctcggc
     1441 atggggatca atgttatgca ggaccggaca gagtggcggg aagacttgag ggacaaggtc
     1501 acgtcctgcc ggctggaagg cgtagagacg gaaccccgcc ggctggccgg gctggtcctc
     1561 gagtcgctgg gccgcgtcta cgccggcttt cgcgctggcc gccgccagcc gcttctgcgg
     1621 gagtggagtc ttcgcaccgg cacctccacc ctgcccgtac tggaatagca gcgaaaagcc
     1681 gcttgcgcac gtgggggacc tccggtaagt tccacaacga agtacgcggc cccgcaatgg
     1741 agaggtggct gagcggctga aagcaacggt ttgctaaacc gtcatactgg accaaatccg
     1801 gtatcggggg ttcgaatccc cccctctccg ccagatgcac acgagttcgt ttccttctta
     1861 actgcttgat ttcagaagga ttatcgagcc tgtcggcacg attgttgctg ttgaacatcc
     1921 catccggcaa tgagaagggc atggctcatc gcggcgatgt gggtgtgcgg ggtgaccggt
     1981 tcgcccgccg acgtcaccca tcctccgccg gcacggcgcc caccaacacg accgacgcgc
     2041 tgctcctcgg cgtcgtcccg tccgcggcgg gccccgagtt gttctggaat ggccgggatc
     2101 gccggcgcta ccgggcatgg gggaccgccg gcgtcgaaga cggagcctgg ccctgaaacg
     2161 ccgctccttg tcgacggccc acggcgtatt tttgaattgg atgaagcacg cgcggaggct
     2221 atacttgcgc cgtgggtgaa tgcgcatgac aaggaggggg tcatgaaatc atcacggact
     2281 gtttcgacag catttgcgtt tgtgctgatc gtgtcctttg cgataccggg cctgtcccag
     2341 gtgccgagcc tggtggccta ccagggccgg ttgctcaacg gcaccaacct gtacaacggg
     2401 cccgccggca tcgtcttccg cgtgtatgac cactccgaca ccaatctcgc cgtgctgtgg
     2461 atggaggcca ccagcgtcgt ccaggtgacg gatggcttct atgccacgtt catcgccccg
     2521 agcaacggcg ccgcagactg ggcctggctc cggagcgtgc ccccgtcggg cgacgcgtgg
     2581 tggctggagg tcgagattaa cggcagccgg ttccagcccc tggagcggat ggccgccacg
     2641 ccgtacgcca tggtggccgg agccgtgacc aacggggcca tcagcggcga catgatcgga
     2701 agccagaccg tgcagagcta caacatcgcg gacgggacgc tccaggaaca ggatttcgat
     2761 tcgggcgtgg tgtcctcccg ggtgatcgcc aacggaagca tcctgtttgc agacgtcaac
     2821 cagaacggcg cggccatcgg ccagatcatc cagtggaacg gcgccgcctg gaccaatgcc
     2881 aacgccgtgg gtttcaagag ctactcggaa aacggcacgt ttgtgatcaa tccgtcagcg
     2941 gtgggcacgg attccatcgc gcagggatac gccacctacg cgggcgcgca ttattccgtg
     3001 gtgggcggcg gcagggacag ccggatcgaa gcgcgcgcca ccaacgcggt catcggcggc
     3061 ggttttggga acagggtggg gaccaattcc ttcgcatccg tgatcggcgg gggctggagc
     3121 aaccggatcg gcaacgatgt ccagctgggc gccatcggcg gcggctatca gaaccgcatg
     3181 gacggcctcc aggcctcgag cgtgatcggc ggcggcaact tcaaccacac gtacaccggc
     3241 gcccactttg ccgtcatcgg cggcggcaag gagaacgaga tatggaccaa ttccgagtac
     3301 gccgtcatcg gcggcggcta cggaaatgac atcgatcccc tggcccgtta ttccatgatc
     3361 ggggggggag agggcaatag catcaagtac ggcacgctgc acggagtcgt tgacgggggc
     3421 tggaacaact ccatcgtgta cgactccgac gaatccgtga taggcggggg acgggataac
     3481 agcattttcg gcgacacggc ctggtcggtg atcgcgggag ggtacaacaa cagcatccag
     3541 gcctccacgc ctcaaggggt catctccggc ggcggcaata accggatcgg cgaggaagcc
     3601 aacaatgccg tgattgccgg cggggccgtg aatgtcatca gcaaccagtc gcagagcgcg
     3661 gtgatcagcg gaggcggaaa ccacgtcatc cgcaccaatg cctcctacgc cgtgatcggc
     3721 gggggagaga acaacgagat tgccgcgaac gcgcatcatg cgacaatccc gggcggcaag
     3781 caggggcggg ccaaccatcc gggggtattc ctgtgggccg atgcctcgag catcagcacc
     3841 tttgcgtccg cgaccagcaa cgaggcggcc ttccgctgtt ccgggggcgt gcggttcacc
     3901 agcggttcgg gagccgccaa tcagaccgtc gcctggacgc cgggctctgc ttcgtggacg
     3961 ttcagcagcg atcgctcgtt gaaagagggg ttcgttccgg tggacggcgg gacggtgctg
     4021 gagcgcgtcg cgaccctgcc gattgccgag tggaactaca tcggttacga ggagcgccat
     4081 attggtccca tggcccagga tttccaggcg gcattccccc tgcgggggtc ctccgacaaa
     4141 gcgttgaaca gcctggacct cgatggcgtg tccctggccg ccatccaggg cctgtacgaa
     4201 ctcttgaaca agaaggatgc cgagatagcg gatctgcgcc gccggctgga atcgatcgag
     4261 agccggttga atgccgacgg cgtcggaccg tgaccgtctc gtcaggcccc cttcgagcgc
     4321 ggtcgcgagc cgacctacgc gcctcgcgca cggcgcccgc cggcagtggt ttttcattcg
     4381 cggcgcgcgc tgaaatttga gacaagcacg tggagatgag atctcgaatc aaagcgggcg
     4441 ccctggttgt ctgggccgcc ctgtgttcgg gcgccatggc ggggccgctt atcctggggg
     4501 ccgagccctt ccgtgacgag gagggaagcg ccgtgtttcg gatatccttc gatcagcccc
     4561 cagacccccg acgtgtccgg atcctgctgg acacggacgg gccggtcagc ggcgagatgg
     4621 tgaccggcgc ggattacatg atggagggcc aggtcctcta tcgctatccc ccgcgcgccc
     4681 gcggatggaa gtgggacacg gtcgagccga cggtaacgct cgtggagggc tcgacgctcg
     4741 tggccttcct ccccgtgccg ccgctgcggg gcgccggcag ctgggcggtc gaatggcttt
     4801 ccggggaatg ggaagtcgag aaccgcctgc cggaatcggg aatggagccc tgcgacttcg
     4861 cctcgctgtc cgaattcagg tggagcacgc cgctccagcc cgaggatatc ggcgacctcc
     4921 tgcgggcggc gtccgcctcg ccgtcggcgg cccgggctgg gaacttcgat tcccccgcgt
     4981 ggagcaacgc gccgccggac tccacaaacg cgccggcccc cgcgccgggt gggatccctt
     5041 tgcaggcgcg gctggtcgac gccgtgaccg ggcaagccgc ggaactccag cccgacgcga
     5101 ttgcgtcgga cggccagcgt tggcgctgga ccgggcgcgc gctcgacatg gagtgggggc
     5161 tgatctggcg gcaggactcg ccgcgggacg tctccgtgtc ggtccgcgtc aagtcggacc
     5221 gggcccgctg tatccgtctc ggcctcggct ggcgcatgga cctggagaag gccatctggc
     5281 atgacgacgc ccgttcccgc gcggccctcg accgttccgg ggcgcgcgtc atgcggcccg
     5341 tggcggtctc gtacggccgc cgcggacggg tgtcggggtt tccgttcggc gtcgtgacgc
     5401 ggggcagcga caattacatg gcggagaccg atccggcgga gccccgcgct tttgaaatcg
     5461 aggccgaccc ggcggcgtcg ttctacggct tgtggtacga cgtggccctg tccacgcagg
     5521 cgtcgaactt cccgggccag gcggaaatcc gttgctcgtt ccgcaccttc gcgggcgagg
     5581 gcggggaggc gttccggaaa gccctggagc ttttttacca gcgccatccc tatttcccgg
     5641 cacggaaggc gcccgtgtgc ggcttcggcc gtccgtggga aaaacccggc ttccctcgaa
     5701 cggtggaatt cgcctgcgag gcccgggacg tgtggacgcc gcccggcccg gaaaccggaa
     5761 caccggccgg atgcatgacg ttcctgttcc tggagccgtg gtcctactgg ctccaggctc
     5821 cggcgcgcct ggcccgcgat gaggcgcgcg cagggaaatg gctgaaactg ctcacggcgg
     5881 ggaccagccg cgacagcgaa ctcgcgtcgg ccgcgcaggt cggcgccgcg cgccggacgg
     5941 acggccgcct cgcgtggcag gaggtcggct tgccctggca taccggcctg ttcctccggg
     6001 tgagcgctca gccgcggctg gcaccgtcgc cggaggctcc actgaaccag gccatggccg
     6061 actggcgcct gtaccggaac gtcatgcaca gcgcgcccgt gcacggcctg tatttcgagc
     6121 cgcccccgga cgccgcgccg atggactaca atcccgccgc cctgggcgtg gctgtccatc
     6181 ctgcaacgtt ctcaacgaat cgtcttcaac ccggcctggc gtcggacctg gaagtggcgt
     6241 cggacctggc cgtctgggcg gcggccgtcc gggcatcggg cggctatacc gccgggcgtc
     6301 cctcggaagc gcaacgggct ttcgccgcgc cgtcgctgga cttcatcgcc gcggatttca
     6361 agacatggcc gggcgacgat gccgattcgg cacgggagcg ttccgccgat tcccttcgag
     6421 cgctggccgg gttgaagccg gtggcgcttc tccgctcgga tgcccaacgc ccgctcgacg
     6481 cggacgaact cgcgcgccac ctggaatggt gcctgttccg gggattcctg ccgggatttt
     6541 acagccgcgc gcggggggcg gaccggttgt atgagcaggc ccagtcggac gaggccatcc
     6601 gcggtatcct ggagaagcac ctcccgctga tccgccggct ggcggccgcc gggtggcggc
     6661 cgtccggtcc cgcggcggtc gagccggatg cgatatgggt ggaatccttc ggccgcgcac
     6721 gtcgtcccgc cggccaggtt acggtgcgca acccgaccgg ggaacgggtg cgcgggaaac
     6781 tgcgcctgcc cggggagaag gggccttcga tggtcatcga tccattgacc ggccattgcc
     6841 atgtgacagc cgatggacct ggcgagttcc cgttggttct catgccccac gagatcgggc
     6901 tccgggattg gtttgccctg tctgaagcga aggaagaagc ggatttcctg gccggctgga
     6961 cgggcggggc gcgggaggcc ccggccgcgc tgaccaacat cgcctccgtc cgcgcggaga
     7021 tggaatgggg cgcctgctgc aatgtgttgg tccccgtgcc cgcggtccgc ggagaccgga
     7081 acgaaatcgt gatcgaggtc cggagcgcgg gcgcggccag attgcgcgcg gttcggccca
     7141 aggtcatcac cagccgggcc ttccgtccgg cggacggcgc ggccgttacc gtggccccgg
     7201 gacagaccga acggctgacc gcggtcttcg aggacggcga tctcgcggac gatccctggc
     7261 ttgaagtcca gtgggaactg gagcgcggag aggaaaaaat gacgtgcgtg cgcctgctca
     7321 agccccgggt ggcggacgcc ttcgtattcg acccgccggg ccgccgcatc gaggccaagg
     7381 gcgggcaggc cgtgatcgag atcccggtcc gcagctactc gaaccgcccg cgtccgctgg
     7441 cgctccactg ggagggcgac caccagggcg gggaccgggc cgtcgaactg cctcccgact
     7501 ccggcacggt gctgcgattc ccgatccgga agaaaagggc ggactccggc cagatgttgg
     7561 tcgaagtccg ctcgggggat tcggtggcct ttcagcgatg gttcgacgtg gtgttccgct
     7621 gatccggcgg gcggaacgcg gttttggatg atgcgcgctt ctcgcatagc gtggtttctt
     7681 cgcctggcgg tcctgtccgg actgctgggc gggaccctgc tcctgctcta ctggaaccgg
     7741 atcggcgatc cgcgtttcta ccgattcctg gagacggatg ccggacgctg gatggcgggg
     7801 accttccgct ggtacggggc agaggcgccg ttcatccccg acgagcgcga cgaatcccgg
     7861 atgactcccg aggaaatcgc cacgttgcga cggatggagg agcgggtgcg ggccgagcag
     7921 ccgacgcacg aactcctgtt caccgacggg caggcgctcc aggggcgggt gatcgaggag
     7981 cggccgcagg aggtggtctt cgtgcgcacg tacggcgaca gcgccgaggt caccgccgtg
     8041 atcccgaggc ggaggctgga atccatccgg cgcctggagc cgcccgcccc gtcgatctcc
     8101 taccgggacg tccgcttcca gatggaattt ccggatttga attactaccg ccggccgccc
     8161 tacagcctgc tcaccgacga atccttcttc cgcgtcgagg agacggtgga aatcctggag
     8221 gttttgcacg agcagttcgt cgggatctgg cggggcctga tggaggaggc ccgccccgac
     8281 cggagcatcc aggttctttt cttctcgcgg caggacgcgt tccgggccta ccagcgacag
     8341 cacgcgccgc gcatggagag ttcggccgga ttctacagcc cgcgcctcga ccgcctggtc
     8401 ctgttcaacc agcgcgggtc cgagcgcgtg gcggaactcc ggcggcaggt ggaggaagaa
     8461 gaggcgcgcc accgcgcggg gcgcctggcc cccgaggtcg aggcccggct gcgcgaatgg
     8521 cgccggcaga cggaccagga catcgagcag ttggccgaaa accagacgcg cgccacgatc
     8581 cggcacgagg gggcgcacca gctgttctac gacttcggca tccattcgcg tcacggcgtg
     8641 gaaggcgagt ggctgatcga ggggatggcc acctattgcg agcattcgcc gatcggcgcg
     8701 gccgatccca cgcggctgaa ctcgctgcgc caggcgctgg aggagggccg cctgatcggc
     8761 ttcgaggagc tcgtgaacct tcgggacgaa tcgggcctgc tctccttcgg ggtcgaagag
     8821 cggatcgggc tcgcgtacga ccaggcctgg gggctggtgc atttcctcat gcagcccgag
     8881 cgacggaacg ccttcttcgg ctatctgcgc tatctccgcg atccgggcgc gttccgcgaa
     8941 atccgcaaga cggggccgtt ggccctgctg tgccggttcc tggagatggc gccggacgag
     9001 ctgtggcagc aatggctgga atacatcctc caaaagccgt cttgatgatc cggtccccgg
     9061 cgcctatcat ctgggcccag gggaggttgt aatcgggcgg gggcccgcgt tcggacggtg
     9121 ccgcaggctc ttcgcaaccg tggagggtcg agcatgccgg gtagacggga atgggtcgtc
     9181 gtggcgctgg ccctgctggc ggcgtgcgcc gagagccgct ccgaggcggc gcgcatcgtg
     9241 cgcgtcggcg tctatcagaa tccccccaag atcttcatca acgccgaggg caggccggac
     9301 ggcctgttcg tcgacctgct ggaaaccgtg gccgcgcagg aaaactggca gctcgacttc
     9361 atgctcggca gctgggcgga ccacctgcgc gcgctggagg agggccggct cgacctgctc
     9421 cccgacatgg cttactcggc ggagcgcgcc gcccgcttca agttgagcca gatcgcggtg
     9481 ctgtcctcgt ggctggaggt gttcgcccgg cacggcctgc cgctcaacac gctggacgat
     9541 ctcgacgggc ggcgggtggc cgtgctggac gagagcatcc agttgcaggt gatccgggag
     9601 cagttgccgg acctcgggat ccaggtggag gcggagatcc ttcccagcca cgacgaggtc
     9661 atggcggccg tgcaggaggg gcgggcggac gcggggatcg tgaaccgctt cttcgacggc
     9721 gcggcggcgc ggcgctatga cgtgatgccg accgcgatca tattccagcc cacgaaactc
     9781 gtcttcgccg cgccggcgtc gagcgacggc cagctgctgg atatcctcga caagcggctg
     9841 gcccggctga agaaggaccg gtcgtcggcc tactaccgga tcctgggcca gtggctcggg
     9901 gaaagcgccc cggcggtccg gtatccgcgc tggatgcgca acacgctcat cgggctgctg
     9961 gcgctggtcg tggccctggg cctgctgggg ctggcgatgc gtcgcgtgat ccgcgtgtac
    10021 acccgggacc tgcgcgacaa gacgcgccag ctcgaggagg ccctgcacga ggtggccaag
    10081 gcccacgagg aggccatcaa gcaagagcgc ctgcacgcgc tgggccgcat ggccagcggc
    10141 atcgcccacg acttcaacaa ctgcctgctg tccatcctgg gctattccga gctgatgctg
    10201 ctcaagccgc aggcggggcc ggacgaccgg gcggaaatcc agcacagcct ggagatgatc
    10261 cggaccgccg cccaggacgc ggcggtgatc gtcaagcgca tgcaggcctt ctaccgggcg
    10321 cagcaggggg atacgcggct tgaaccggtc gacctgaacg ccgtggtccg ggacgcggtc
    10381 gaattgagcc ggccgagctg gaagacgcag gcggaatccg ccggcatccg gatccacgtg
    10441 gagaccagcc tgggccagat ccccttcgtg cagggctcgc cgtcggagct gcgcgacatg
    10501 ctggtgaacc tgatcatcaa cgcggtgcac gcgatcgagc gcgagggcac gctgcgcgtg
    10561 tccacgagcg cccgcggcga gtgggtggcc ctggaggtca aggacacggg ctgcgggatg
    10621 acgccggaag tccgggcgcg gtgcatggac ccgttcttca cgacgaaggg ggaaaagggc
    10681 accggcatgg gtctcgcgat ggtgcacggc atcgtgctgg actaccaggg gaagatcgtc
    10741 atcgacagcg agcccgggaa aggcacgacc gtcaccatcc tgcttccgat ctcgtcgaaa
    10801 gcggcggccc cggtgccggc ggccagggcc ctgccgggcc tggggccgcg gcggatcctg
    10861 gtcgtggacg acgatccgcg cgtgctgaag acgatgtcgg ccatgctgcg ctcggtgggg
    10921 cacgtcgtgg aggtggtctc ctccgcccac gaggcgctgg accgcatgga ggtggggcgg
    10981 ccggacatcc tcctcaccga tctctccatg ccggacatga ccggcatgga cctgctccac
    11041 gaggtccggc ggcggggctg gacgcttccg tgcctcatcc tttccggcac gcccgaggtc
    11101 gcgcagaagt attccgacga cccgatcccg atcctccgca aaccgatttc cctgacggac
    11161 ctgaaccgcg ccctggccga ggccctcgca tccccgccgg gatcggcggg ttgacgccgg
    11221 cgcggattac tgtttccccg ttggaacccc tttgttatag tgccctgttg tccggagctt
    11281 ggacggcccg gcgcggccgg agatggccgg gaaggagtct tcatgcccag attggaaaac
    11341 gacgaaattc agaaagtact gccccatcgc ttccctatgc tgctggtgga ccgcatcctg
    11401 gagtgcgacg acaaggagcg cgtggtcggc atcaagaact tgagcatcaa cgagcccttt
    11461 ttccagggcc atttcccggg gcatcccgtg atgcccggcg tgctccagct ggaggccatg
    11521 gcgcaggtgg gcggcgtcct gttgagccgg gtgctgaacc agccggggcg catcgcgtat
    11581 ttcatggcca tcgacaacgc gcgcttccgg aagattgtcc aacccggcga ccagctgcgc
    11641 atcgaggtga ccttcgagaa gctgcgcatg ggcatggcca aggttcacgg cgtgtgcagc
    11701 gtggacgggg acacggccgc cgaggcggac ctgatgttcg tgtataagtg aggacggcat
    11761 ggcgcgcatc catccgacgg ccatcgtgga tccgggcgcg gagctgggcg aaaacgccga
    11821 gatcgggccg tactgcgtcc tcggcccgca cgtccggatc ggcgacggcg cccggctgat
    11881 ggcgcacgtc ttcgtggacg gctggaccac gctgggggcg gggtgcgcca tttttccctt
    11941 cgccagtctc ggcacgcaga cccaggatct caagttcaag ggcggcgcgc cccgcgtgga
    12001 gatcggggag cggaccacgc tccgcgagta cgtcacggtc aacgcggcca cggcggacgg
    12061 cgacgtgacc cggatcgggg accgctgcca catcatggcc tacgcgcata tcgcgcacga
    12121 ttgccgcgtc ggcaacgagg tcatcatggc caactgcgcg acgctggcgg gccacatcct
    12181 cgtggaggac caggcgatca tcggcggatt gtgcggcgtg caccagttcg tccggatcgg
    12241 ccgcatgagc atcatcggcg ggtgctccaa ggtcacgcag gacgtgcccc cgttcatgat
    12301 ggccgacggc cacccgctgg cgatccacgg gatcaacagc gtcgggctca agcggcgcgg
    12361 cgtgggcgag gaggcgcagc gccagttgaa gaacgcctac cgcatcctgt accgcgaggg
    12421 gttgtccacg agccaggcgc tggagcggct ggccgcggcg ggcgcggagg ggccggaaat
    12481 ccgggagctt gtcgagttca tccgccactc gaaccggggg atcgtgcgct aaatgaaaat
    12541 gaggatcctg ggcctggccg ttctgctggc cgccgcgtgg gcggtccggg cggcgccccc
    12601 gtcggcgccg atcgaaccga gaatcaactt cagcttcgac caggtggacg tccgcctgct
    12661 ggtcaagctg gtgggggacc tgaccgggcg gcgcttcgtc gtggacaagg gcgtggcggg
    12721 cacggtcacg gtcatctccc cggcgccggt cccgtcgtcc gaggtgtatc cgctgttcgt
    12781 ttccattctc gaggcgcacg gctacaccgt ggcggagaag gaaggcctgt gctacgtgat
    12841 gccgctcccg gaacgcgggg tgacgggcgg gcgggtcatc gggccggaag gcagcgtgcc
    12901 ggccggcggg ctggtgaccc gggtgatccg cgtgcagtac atcagcgccc tggaactgcg
    12961 gaagatcctc gagcccctgg tgcggggcgg caagagcggg gccctggcgg ccttcgggcc
    13021 gtccaaccac ctgctggtga ccgacacggc cgacagcgtc aagcagatcg agcgggtcgt
    13081 ggccgaactg gaccggccgg gggcggggga gacggccgag tttatcccgc tggagcacgc
    13141 ctcggcggag gaggtggccg cccagctcat gacggcgatg cgcagcctgt cctcgtcggg
    13201 cgccacgttg agccggcacg tgcagcaggt cgccgagggc ggggcctcgc tgcccgcgga
    13261 ggtggcggtc atcccggccg cctcggcgaa cggcctcgtg ctcgtcgggg cgcccgtgca
    13321 gatcgcggag ctcaagcgga tcgtcgcgat gatggacgtc gagccggtgg ccgggaaggg
    13381 gcggctccac gccgtcttcc tgaaatacct ctccgcggac gaggccggca agagcctgaa
    13441 cgcgctgctg gccaagacgg ccgaaaagga ccagcgccag cggatcgcca tcgagcccaa
    13501 catctcgaac aacgccctga tcgtcgaggc gtcggcccag gatttcgagc tggtcaaggc
    13561 gctgctcgcc gaactggacc aggtgccgca gcaggtcctg gtcgaggtgc tgatcgccga
    13621 ggtcgcgctg aacaagaacc tggacctggg cgtggagtgg gcgatcatcg aatcgccgaa
    13681 ggacggctcg accacgcccg tgagccgctc ccggccggga gacagcgacg cggtcatgga
    13741 tatgatcacc gagggcgtct tcccgcaggg cctgtccgtc ggcgtcgcgc gcggcacgta
    13801 cacggacctg gccggcaacg aggtgcccaa catccccatc ctgatcaagg ccctggcgca
    13861 gaaccgcgac gtgcggatcc tctccaacat cccgctctgg gcgcagaaca acacggaggc
    13921 gagcctctcc gtcgtggaga acatcccggt cctcaagtcc accatcgagg gcggttccgg
    13981 cacggcgcgg gacgtgatcc agaacatcga ccgcatggac gtggggatca agctcaagct
    14041 caccccgcac gtgaatccgg atcgcgaggt gctgatgcag ctgaacccca gcatcgaggc
    14101 gatcgtcgac gaggggcccg aggataagtt cgcgccgacc atcgccaagc gggaggtttc
    14161 aaccacggtc accgtgcccg accgcgcgac ggtcatcatc agcggtctca tccgcgagga
    14221 cgagatgaag gccgtcggca aggtgccgct gctgggcgac cttcccctgg tgggctggct
    14281 gttccgcagc caatcgacgc gcaaggtgcg gacgaacctg ctgatcttcg tcacgccgca
    14341 cgtcgtcacg gacgtccgcg aggcggccgc gatgaagaga gacctggagc gccaggcgca
    14401 gctgatcaac gcgcagacga acctgaacca gcggccggcc cggaggaagt aagcgatgcg
    14461 cctggacgag gtgctggtgg gccggggcag ggtgacgccg gagcaggtgg aggaagcccg
    14521 cgcccgggcg gggaaggcgg gcggcgacgt ggagctcggc cgggcgctgg tcgacgccgg
    14581 gctcctggcc gacgccgacc tgctggacgc gctgggcgag ctctacggct gcgacgtgct
    14641 gcgcgagccg gcggacaacc tgctcgaccc ggaactggtg gcgcaactgc ccgtggagtg
    14701 ggcgcgcgcc catgccatgc tgcccgtgcg ccagggcgcg cgcgtggtcg tgctgaccag
    14761 cgatccctcc gacctcggcg ccctggatga cctggcgctg atcctgggca cggaaccggc
    14821 gcccctcctg gcctcccgcc gcgaaatcct ggcggccatc gagcactgct atgtccggaa
    14881 gacggattcg ccgggcgacc tgctgcgcgg catgaaaccc gcggccgggg atacccccga
    14941 gcggccggcg gacgatctcc tgcgcacctc cgagcaggcc ccggtcacgc agttgatcaa
    15001 tctcatcctg ctcgaagccc tgaaggcgcg cgcatcggat gtgcatctcg agccctatgc
    15061 cagccacctg cggctgcggt accggatcga cggcatgctc tacgagcaat cctcgccgcc
    15121 gaaacacctc gaggcgccgc tggtttcccg cctgaaggtg atggcccggc tcgacatcgc
    15181 ggagaagcgc ctgccgcagg acggcacggc gcgcgtgcgg gtcggcgagc aggaggtgga
    15241 catccgcgtc tcgacgatcc ccgtggccga gggcgagcgg gtcgtgctgc gcctgctcaa
    15301 ccgggaatcc accgtgctgc ctctctcgaa actgggcatg ccggattcgc tgctggcccg
    15361 gttccgcgaa ctggtggccc agcccaacgg cgtgatcctc gtcaccggcc cgacgggcag
    15421 cggcaagacg accacgctgt acgcggcgct ccaggaactg gacaaggcgc acgccaacat
    15481 cctgaccatc gaggatccca tcgagtacca gctcccgcac atcgggcaga tccaggtcaa
    15541 gccgaagatc ggcctgacct tcgcgtccgg gttgcgccat atcctgcgcc aggatcccga
    15601 cgtgatcctt gtgggggaaa tccgggacgt ggagacggcg gagatcgccg tgcgcgcctc
    15661 gctgaccggg cacctggtct tcagcaccct gcacacgaac gacgcggtca gcgcggtgat
    15721 ccggatggcg gacatgggcg tggagtcgta cctgctggcc gcggcgctgc gcgcggccct
    15781 ggcccagcgc ctggtccggc gcctgtgccc gcattgccgc cggcccgcgg tcatgacgga
    15841 cgagcaagcc cgggccctgg gctcgcgggg cggggagtgg gttgggagcc cggtctggca
    15901 gcccggggaa tgctcccggt gccggggcgg ataccatgga cggctgggcg tgtttgagct
    15961 catgctggtg gatagcgccg tccaggaagc ggtgcgggcc ggccggccgc tgggcgagat
    16021 acgggaacta gccgcgcggc aaggcatgcg cgcgctgcac gacgacgccc tggacaagat
    16081 ccggtcgggc gaaaccagcg tggccgaggt gctgcgcgcg gtggggcgtc acgacgtccg
    16141 cggagccgcc ggatgaagac gttcgcgtac cagggattcg accattccgg ccggtccagc
    16201 gcgggattgg tggaggccct ggacgccaag gaagcccggg aaaagctgtt cgcgcgcggg
    16261 atcctcgtgg aagggctgcg tccggccggc cagggaaagg ccggccgttt ccggcggaca
    16321 ggtgcgttcg acgcatcggc ccgcgccatg gtctaccggg aactggccgc gctcctcaag
    16381 gccggcctgc cgctgaccca ggccttggaa atcatcctgt ccgcccccga gacgggcgcg
    16441 caccagatgt tgctggccga tacccgcgat cgtctgcgcg aaggctcgtc cttcgcggaa
    16501 gccctggccg cacaccgggc ggtgacgccc ttcgagaagt ccgtggtcca ggtgggggaa
    16561 cgaaccggca ccctggatgt ggtgctggac cgcatcgcgc gtttcctgga agaggaaagc
    16621 caggtccgcg aacgcatcca gaccgcgctg ctctatccag tgatcgtcct gatcatggcc
    16681 atcgcgatcg gcatgctcac gatgggcgtc atgctgcccc gcatggcccg cctgctggaa
    16741 gaggccaaca tggaccttcc cgccatcacg cgtttcaccc tggcgctggg acgctggagc
    16801 gccccggtcc tgcttctcct catcgttgcg ctgggcctgg cgggcggttg gctggtccgg
    16861 cgcatgcgcg ccgcgccgga ggtccggcag ctatggaatc gacgggcttt tcgctggccg
    16921 ctggcggggc ggatgtacac ggccctggtg aacctgcggt ttgcgcgcac gctggccctc
    16981 ctcctggacg ggggcgtgcc cctggtggag gcgctcccgc tggccggccg ggcgaccggc
    17041 agctcctgga ccggccgcct ggcggcggaa caggccgagg ccatgcgcca cggccgcaac
    17101 ctggccgatg cgctccgcca gatcccgccg ctggctgggt cgctgccggt atggatccag
    17161 gccggcgagg ccggtggcaa actttcggtg ctgctcgaac acgccgcgga ccgttaccag
    17221 cagcagtgga gccgggtcat cgcccgcagc ctcggcctcc tggaacccct gctgatcgtg
    17281 ctcgtgggcg gttttgtgct gctggtggcc ctggccaccc tgctgccgat cctgtccctg
    17341 aaccagacgc tgcactagga ggacatcatg cgcccgttca atgcgctgct ttcgatcggc
    17401 ctgaccggca ccttcctttt cggcagcacc ctgctggccg gattggtgca actgcatcgc
    17461 ggtgatcccg ggcacaactg gactcaccgg gatatgcgcc tttcgttggc cgaaacacgc
    17521 aaccggaccg agatctatat cggcggcgaa ctgctccagg atcacctggg acggaaaggc
    17581 ctgacggcca cgggccctga cggcaccgtg tacccggtcg tcgaaaggga catccgcatc
    17641 cgattgaaca actggccgga gcggcgagcc gaactcgcga tgcgcgccat cctggaggca
    17701 tttttaacgg gcctgtccgt ggccgccctt atgatcggca tcgttcaggc gcgccggcga
    17761 aaccagccgt gagagagccc ggctgcgcac cggatgggtg atcgcccttg ccgggctgct
    17821 cgcgggctgc gcgggcgttc gtaccggcta cttcagccgg ccctacaccg gcgcgccagg
    17881 gcggtgtgcc gggccttcca tcgcttacga acgcgaccgc atgctggaga tcagtctgcc
    17941 cggactctcg atgcacatcg acctgctgaa cagctggcag ctgtccgact cgatctggtt
    18001 cggactgggc gtccccctga ttccccttcg ggtcgagcga tgcgagccca cggggatttc
    18061 acagccgggc taggtactgc tccatggccg ctatgccttc cggcgttccc atgtccagca
    18121 gggggggggg cgcggggaag accgccagca gcccctgcgc cgcgagagag gggaagacgt
    18181 ctgtttccag cgaaaggttc cggccgggct ccagctgcgc cagcaggcgg cgggggatca
    18241 cgtagacgcc gccgttgacc cagccggatt cgcgcttcgc cttttcgcgg aaggcggtca
    18301 cccgtttccc ctcgtcgaat tccacggtcc cgtagcgccc ggcttcctcg atccgggtga
    18361 cggccagcat cgcgggcggc tgtttccaat cattggaaat gctgcgccat gcctttccca
    18421 atgattggaa atccaggccc ggcagcaggc tgtcgccgtt cagcgccagg aacggatcgc
    18481 tcgcgatccc cggctccgcg tatttcaggc cgccgccggt ccccagcggg gccggttccc
    18541 ggctgatggt aagcgacagg gcgcgcggcg cttccgggct gtccagccat gcctcgatcc
    18601 ggtcggccat gtgccccccc gcgatatgga cccgccggac cccgccacgc gcgagccagt
    18661 ccaactgcca ttcgaggaac ggccggccgg cgacggggac cagcgccttc gggcggtccg
    18721 cccacaccgc gctcaaccgg gtgccccggc cgcccgcgag gatgacggct tgcatcatga
    18781 cacgtactgc ttgggcttga tcttgtactt catgatcttg tacccgagga tgcgctgggt
    18841 gctctcgagg gtccgcgcgg cggcggcaac gttcccgcgc gacgacttga gcgcgtcccg
    18901 gatcaagtcc cgctcgtagg actccacgag gcccttgagc gcgcccgatg gcgccgtgcc
    18961 cacggcctcg gccgtctgga gggtcggggg caggtggtag ggatgaatca cgtcgccctc
    19021 ggctacgagc acggcccgct cgatgcagtt ctccagctcg cgcacgttgc ccggccagtg
    19081 atagctcatc agcatgtcga tgaccgcgct cgagagccgc cgcaccttct tgttgttttc
    19141 cttggagtac ttctcgagga aatggtcggc cagcaggacg atatccgcct tacgcttgcg
    19201 cagcggcggc acgtagatgg ggaacacgtg cagccggtag aacaggtctt cccggaactt
    19261 gcccgccgcc accatcgccg gcaggtcctt gttcgtggcc gtgatcagcc ggcagttggt
    19321 cttgatcgtc gccgtgccgc cgatccgctc gaactcgcgt tcctggacaa cccgcagcag
    19381 cttgatctgg atggacagcg ggatgtcgcc gatctcgtcg aggaacagtg tgccgccgtg
    19441 ggccagctcg aatcggccct tgcggtcggc gagggcgccc gtgaaggcgc ctttgacgtg
    19501 cccgaagagc tcgctctcga tcaggttttc cggcagcgcg gcgcaatggg ccttcacgaa
    19561 cggcttgtcc gcccgctcgc tgttgtagtg gatcgccgcc gcgaccagct ccttgccggt
    19621 gcccgtctcg ccctcgatca gcacggtggt cggcgtcttg gccacctggg cgatctggtc
    19681 gtagacgatc tgcatctcgt gcgagttgcc gatgatgttg gagggccgga accggctgcg
    19741 cagctcctcc cgcagccgct ggttctcgat ctcgaggcgc tcctgctcct cctgcgcgct
    19801 gcggcgcagc ttgaccgcct gcgcgatcat ggaggcgatg atggtcagca ggcgcacgtc
    19861 gtcctccagg ttggccgacg ggcggtaggg caggtccacg ctcagggccc cgacgacctc
    19921 ctgctcgacc ttgatcggca cgcacaggaa cgacgtgtcg gcgcgcttgc cccggcgcgt
    19981 cttgtcgagg aacagcggcg attcgctggt cttggggatg atcgccggct tcccggtgag
    20041 cacgacctgg ccggtaacgc cctcgcccag cttgtaccgc ccccgccgcg cctgctgcgg
    20101 agacaggccg tgcgccgcct cgatcaggat gtcgccggtc ttccggttga gcagggtcag
    20161 cgtggcgtac ttcatgtcca tgtattcgga cagcgcctcg agcacggggg tcaccacgtc
    20221 gcgcatgtcg aggctctggt ccagcacccg gctgacgtgg tacagcaggg tcacctcgcg
    20281 catgcggcgc gcgtcttccc gcggggtcgc cggggcagcg ttcatggcag ccgttctcct
    20341 tccgctcgct cgtcgtataa attggtgatc ggcatccggc ggtcccgccc gaaggccttc
    20401 ggcgtgatct tgatgcccgg cgcgccctgg cgccgcttgt actcgctgcg gtccaccagg
    20461 cgggccacgc gccgggccgt ggccggctcg aacccggcgg cgacgatcgc cgggacgctt
    20521 tcatccagct cgacgtaccg ttccaggatc gcgtccagca ggtcgtaggg cggcagcgaa
    20581 tccgcatcct tctggttcgg ccgcagctcc gccgacggcg cgcgcccgat cgtggagggc
    20641 gggatcggcg ccttccccgg cggccggttc cgccaccggg ccagttcgaa gaccagcgtc
    20701 ttgggcacgt ccttgatgac ggcaaacccg ccgaccatgt ccccgtagag cgtgcaatac
    20761 cccgtggcca actcgctctt gttgcccgtc gtcaggacca gccacccgaa cttgttggag
    20821 agcgccatca ccaggttgcc ccggatgcgg gcctgcaggt tctcctccgt cacgtccggc
    20881 gggcgcgccc cgaaggccgg cgccagttcc tccacgtagg tttcgtacag ccggcggatc
    20941 ggcagcgtga acagttcgat ccccaggttc cgcgccagga ggccggcatc gcccagcgtc
    21001 tcggccgagg agtactgcga gggcatcgtc acgcccttga cccggtccgg ccccagcgcg
    21061 tcggaggcca gcgtcgcgac cagcgcggag tcaatgccgc cgctcaaggc gacgaccacc
    21121 ttgccgaagc cgttcttgtc cacgtagtcc cgcaggccca gcttcagggc ggcgtagacc
    21181 tccgcgggat cctccagcgc gggctccacg cgggcggcgg aggtcgatgc ctgccgcggc
    21241 gcgacgctgc tccgtatgcc caggctgaaa acctgcgaag gggcaggggg cgggccccgc
    21301 ctcccgactt cgccggccac ggtcacgagc agcacatctt cctcgaattg ccgggcgcgc
    21361 gccagcagga ccccgtccct ggacagcgcc aggctggcgc cgtcgaagac cagctcgtcc
    21421 tggccgccca ccaggttggc gtagagcacc gggcagccca gttgccgggc cgcctcgcgc
    21481 aggacgtttt cgcggagcgc gagcttgccc cggtggtagg gcgaggccga gagattcacg
    21541 agggcgtcca gaccctggtc gcggagcagg cgcgcagcgg cctcgtcgag gtcccaggag
    21601 tcctcgcaga tgtggacgcc gatccgcaag ccgtcgaggt cgaggaagaa cgcgcggtct
    21661 ccggcttcga aaacgcgctt ctcgtcgaac accccgtaat tcggcagcac catcttgcgg
    21721 tagcaggcgg cctcccgccc cccgtgaaag accacggccg tattggccgc cttttcgccg
    21781 gccgcccggg gacacccggc gatcacgacc gtttccggcg ggagttcacc cgcgaggcgg
    21841 cggatctgcg cctcgcaatc ctcgagaaaa tgccgcttga ggaccaggtc ctcgggcgga
    21901 tagccggaaa gcgagagttc cgggaagacg accagcgacg cgccccctgc ggcggcctcc
    21961 cggctcacc
//
LOCUS       DAOOTK010000045        21930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1635_length_21930_cov_5.658286, whole genome shotgun sequence.
ACCESSION   DAOOTK010000045 DAOOTK010000000
VERSION     DAOOTK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21930
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1635_length_21930_cov_5.658286"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1970
                     /locus_tag="PK634_06960"
     CDS             288..1970
                     /locus_tag="PK634_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97512.1"
                     /translation="MPLHAATNYVNVANPTPVAPYTNWAMASTNIQLAINRAAAGGLV
                     LVTNGTYTGARKSVYPYGYTMLAVTNGVILRSVNGPEATTINGLGDRRGAIISNATVE
                     GFTFTNCVGDGILAYKGGGAALVGGWGRLNNCILTGNRGVNYGGSAAVFSLGIISNCV
                     IRGNRATTAGGGICMYEGGLVVDCVITNNFVGGAAGARGGGIACEIGGVVSNCQIVNN
                     LSWMQGGGISIFTNGTAANCLIAGNTANTNNLEGEGGGAYLHMGGLLRNCVISNNFGL
                     WQGGAVKFYYMGGVVRGCLIRDNSTLSWGGAMMMRSGGRAESCTMVHNTGGECGGIYF
                     YAMGGTSLNSIVYHNTGGDLTNAYGGSVLYSCVPNTGFGAGTITNDPAFVDLAGGDFR
                     LSTNSPCINRATNQAWMSGAQDLAFAGRVQNDIADIGALESSFWSRYEDADADGLSDG
                     DEAPMGTDRLDPDSDDDSMNDGDEVYAGSHPTNASSFFQVETPDEAPGEFPVIRWDSV
                     AGRTYRLERTTNLSSSAFSIFQSGIPADPPVNTHTDATAAAEGPWFYRVVVE"
     gene            2113..3960
                     /locus_tag="PK634_06965"
     CDS             2113..3960
                     /locus_tag="PK634_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97513.1"
                     /translation="MQTRLTRGNAMLLLAVAVIAVYANGIQGPFVLDDSYSIVENPAV
                     RALWPPWMALNPPPVNMTFYARPVINLTACVDYAIWGLNPMGFRLTNVIFHLLATLAF
                     FSLVRRTLATVGRSDGEAVFIGFAAALLWAAHPLLTAAVNYPSQRGELAVGLFLFLML
                     YALNRAALADSTSSRSWLALSVLLCLLGMGSKETMAAAPFLALAYDRVFLAGSWREAL
                     RRRWGFYLLLALTWIWPLWKHLTFSPQAQGDVMNPTTWHAYLLTQTWGLNRMIRLMLW
                     PHPLIFDYGLGMALRLADVRAAAIGLVLLMGFGAWSWFRFPRVGFAALCFFAVLAPAS
                     LFPVTGQPIAEHRMYVPLAPAMLLLVLGAEALGRDRRWNARHAGLALAAAAVILGGLT
                     AHRNTRFRTAAALWTDTIAKWPGNARAWYNRGYYYQGQGQTDAALADYARSLELNPRY
                     VNPLVNRAKIHFDRRDLPAAAADLDAALAIMPDWPSILTRRAMIRRLLGRHEEAAADL
                     DHAARLEPENAVFINELAWVLATAPDDAVRDAPRALELATRAAALTDRDYHALDTLAA
                     ARAANGQFEAAQALAREAVAQAQQQGDPAAARRIGERLARYEQGLAWRE"
     gene            4061..4792
                     /locus_tag="PK634_06970"
     CDS             4061..4792
                     /locus_tag="PK634_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97514.1"
                     /translation="MTTTLFIPTLNEIESMRQILPRIRREWVDQILVVDLSTDGTAEY
                     ARGQGCEVVRQQVKGLRHAYREGFPHVRGDVVITFSPDGNSIPEVIPALIAKMKEGFD
                     MVIASRYRPPAKSADDDWMTGFGNWLFTRAINLLHGHAWGRPYTDALVMFRAYRTSLY
                     RELELDLDDGYAPERWFRTIMGVEPLLSVRAAKRRLKIGEVPADEPARIGGTRKLQLV
                     RWPCAYMSQVIRETWRWRKKAGPPA"
     gene            4789..4980
                     /locus_tag="PK634_06975"
     CDS             4789..4980
                     /locus_tag="PK634_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97515.1"
                     /translation="MKKRPTSRRRSRAKKPADRVVLSRAEYEALLRDARAKAVYDEIA
                     AYLLNGIETLLNDESPPAP"
     gene            4977..6494
                     /locus_tag="PK634_06980"
     CDS             4977..6494
                     /locus_tag="PK634_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97516.1"
                     /translation="MNISLVYPQWTEEYGQIAHFAKKAGKWPPLNLAYLAAIAEAAGH
                     RVRITDAEAEALPMETTVDRVLAEKPDLVGVTATTPFYHIACDLAAQIRKRSAAPIAI
                     GGPHVTVLREAAFPREMDFAFIGEADRTWPAFLERFADGRDPGDVPGLLYRQDGKVRY
                     TGPALPVEDLNALPVPARHLLKADRYFLGTLEGAKRFTSIMTVRGCPYRCIFCSTKVF
                     GRDTRRRDPPLVLDEIRDCVERFGIEHFMFMDDTLTLHREHIRAICRLLIEARLNITF
                     EGSTRANLVDEETVRLMRKAGLIRLSFGLESVDPGIRKTMRKQVPLESYIQANRLTRK
                     HGIETLNSCMIGLPGETRETIRATLRFLRESREIEQANISIAVPYPGTELYEMARRGE
                     MGLRLETEDFSQYRRYNAAVMTVGDLSPADLVEIQNEAFASIYLAPWRWRPMIAKSGA
                     MGAVLTARRLARALKEGRSDFLTDRQLGISAAAPSLPPSAPDAEPGDPDSSELVH"
     gene            6616..7803
                     /locus_tag="PK634_06985"
     CDS             6616..7803
                     /locus_tag="PK634_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOW97517.1"
                     /translation="MTTTFKSKRYDLAVDTITEADIRELIDWLRAYPRLTMAHVTREF
                     EKAWSEWLGVRYSVFCNSGSSANLLMYAALDSTGRSRNRGIVVPAVGWSTTVGPSLQL
                     GWKPVMCEADPLRYSLDARALERLLREHRPDNVILVHVLGTPADMEPILALQEKYKFN
                     LMEDCCAAHGARFRGRKVGTFGAMSSFSFFYGHHMSTIEGGMVSTNDEELYHHLLMLR
                     SHGWCKDLPPEKYRELMAEHGVDSFHCPFTFILPGFNLRPLDLTARIGLSQLPRLDET
                     VRRRAENHRRYQQRLEGAVEFARVGEADLISSISFGGVAASNAQRKVIVSALVKNEID
                     TRLFSAGNLGRHPFWTKRHGVFSAPVADRIHDGGFFLPNNQSLDAGDIDFVSDVVIKA
                     VRG"
     gene            7821..9323
                     /locus_tag="PK634_06990"
     CDS             7821..9323
                     /locus_tag="PK634_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97518.1"
                     /translation="MASDILFIHPGNQKAIYQDLAREFTAVDPPIWALLLAGALRRKG
                     LDVAIHDAHIQGWDADIARDALRRHEPRLAVILVYGHHPSASTQTMPAARAALAALKE
                     AAPDLPVALGGTHPSALPEQTLREEPADFIVQGEGLHTLLGLAAHYHGKQRPEDIPGL
                     WRRTENGPAFNGPAPVARKLDEELPEYAWDLLPGLHNYRAHNWHCFQDFARSKAPDFS
                     DVRSPYASIYSSLGCPYDCDYCCIHTLTGGKPGLRPWPIEHVVEVAAHLAQKHGVRNI
                     RLADELFVLNPARVERFCDRMIERGLDMNFWSYARVDTIPEALLPKMKKAGLNWLCLG
                     IESANERVRASVNKMLRRDIVSTVRKIQDHGICIIGNYMFGLPEEDAGTMQETLDLAL
                     ELNCEFVNFYTVMAYPGSRLYAQARAAGTVPDRWEAFSQHSFYTQPLPTRRLTGAEVL
                     AFRDRAFETYFRDARYQAMIRRKFGPAVCAHLEKMLGFKIRRKLLESASA"
     gene            9354..10181
                     /locus_tag="PK634_06995"
     CDS             9354..10181
                     /locus_tag="PK634_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOW97519.1"
                     /translation="MAATLKAKAYELKKTMLETCIRAGTGHVTSSLSCAEILTTLFHG
                     GALRFDPKRPAWEDRDRFILSKGQASPMLYTVLGDLGYFPKEDLQKFAQAGGPFGVHL
                     QKSVPGAEITCGSLGHGFGLAAGMALAARMDRRNHLVFTLLGDGECYEGSIWETALFA
                     GHHRLNNLVAIVDRNYQCVLDFTENFLALEPMEEKWEAFGWRVKRVDGHSFDDLLDAL
                     KGVRSRRSVQPLVILADTVKGAGVGFMACNPIWHGAAPRGDEAQQALRELERSARDE"
     gene            10174..11103
                     /locus_tag="PK634_07000"
     CDS             10174..11103
                     /locus_tag="PK634_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOW97520.1"
                     /translation="MNNVSQRDAFWTRLYEVARQDRRVIAVAADMGAPALDQFRRDLP
                     GQFVNAGIAEQNGLLVAAGLALEGKHPFLYAIAPFATFRVLELIRVENSIMDIPVTIV
                     GVGAGFGYEDSGPTHHILEDIAALRAFPHVTIHSVSDSTMAAAVAEHSCGMKHANYVR
                     LDRAVLPELYQAAPDLAKGFAVLRPSKAGWLIGTGYMTHKALRLANRLAGEGLDLGVM
                     DLFAMPFDEKAFVAALGKAPRLFTLEEHFLAGGMGSLVLETLNRHGLAIPVKRFGLEM
                     DKGYSYVYGGRDRILAGYELSEDDLAKAIRNWK"
     gene            11223..12962
                     /locus_tag="PK634_07005"
     CDS             11223..12962
                     /locus_tag="PK634_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97521.1"
                     /translation="MSGNASSAPPPARLRWGPALLIFLSVFVAFGGALRNDFVRFDDD
                     LYVTGNPHVRAGLTREGVRWAFTSMHDANWFPLTLASHMLDVSLFGLNPAGHHFTSLL
                     LHALNGALLYGLLRRLTGRAWPAAFAAAVWALHPLRVESVAWVSERKDVLCALFGLLT
                     LLAYERYARRPTAGRYLATGLYFALGLMAKPMLVTLPFVMLLLDYWPLGRGPAEGWRH
                     LALEKLPLLAIAATACVVTFLVQRAGGALKTIDQFALAVRAGNAVAAYAEYIRLLLWP
                     ANLAVFYPHPGVVPPWSRLLPAMAVLLGFTALALRLGRRQPHLPVGWFWYLGMLIPVI
                     GLVQVGIQSHADRYTYLPHIGLLILLAWSADRWLGERTTARMAAFAVLLPLAWAAHRQ
                     TATWRDSESLFLRAIAVTGPNAVARNNLGAHYATQGRRAEAEEHLRRSLEIQPGYADA
                     QFNLANLLLEAGRRPEAIAEYRATLEYRPHHLPSLNNLAWLLATDPDSNAEDRAESLV
                     LAERAIGPTRSPDAGLLDTLAAALAANGRFEEAARAAAQALDLARAAGQEDLARRLER
                     RLAAYREGRIRRE"
     gene            12959..14449
                     /locus_tag="PK634_07010"
     CDS             12959..14449
                     /locus_tag="PK634_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97522.1"
                     /translation="MKGDVLACTILALAPLVSAAALAGLIRFSRRADGRRRDAVTWLA
                     RIMIPVFLVSAGLAAGEAYFRFFHDASDSYGLSRVARRWFERHWRLNRSGFRDDAEYT
                     IQCAPGRRRITFVGDSFTAGHGLANVRDRFANRLPDGRAYEVHVLAKAGYDTGEMLEL
                     LQDLRAQGYGMDEVVLVYVPNDISDIAPGHARMNRRIFARPAGFWVNHSLLFNTLYHR
                     GRARTDPDVAGYYRYVEKAYSGPTWEEQQRRLLALRDLAESQGGRFRVVTFPFVQGLG
                     AGNEFRAVHAKLATFWTGQGVPHLDLLGVFESAPGRRWTVNRYDAHPNAAAHALAAEA
                     IGAFLQTGARPAAPAPSAGPAGPLLQPILTTKERAQRGAAELAQSRFAEAVADWRIAL
                     AARPDSAEILNNLAWILATCRDPALRDGAEAILLAERACALATPDEAGFRDTLAAAYA
                     EAGRFDEAIRAAELARDIARHAGDEALAARTEQRLVGYRRGRPWRD"
     gene            14558..16606
                     /locus_tag="PK634_07015"
     CDS             14558..16606
                     /locus_tag="PK634_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97523.1"
                     /translation="MLALLLALLTFALFRPAARYGYVKLDDDQYVASNLRVTTGLTAD
                     NVRWAFHTVYEDWWLPLLWMSFMADSEVYGPVPAGYHTTNILLHALNAGLLCWILFRL
                     TGSRWRSFFCAALFAVHPLRVESVAWITARKDVLSGLFFLLGLLAYVRYVERPGAARF
                     AVLAALMLMGLLSKAIVLVLPFVLLLLDYWPLRRAGDPRDPAGLKTWRGLLAEKIPLF
                     LLAGVFILINLGTHVAGRGADSNLSAATRLGLIPPNYWAYLAKICWPVRLVLFYPEHD
                     VVRWPVTLAALAGLLGVTLLLLRCRKAAPHLAVGWLWFLVALFPVIRGVRLGLAAYAD
                     RFAYLPSIGLGLALVWGVAALAERRRSLAAAAALAGLLLLGAGAWKTRAQLPLWADSF
                     TMFGRLVRLEPDCDLANNSFGKALMERGPLAEALPYFKKAAALNPAGSRGVVNYADAL
                     IRLGRDDEAIAWLQDARAKGYPEVTELDVLLGFAYLNTGRAEEAVPRLQRVSRNRPEQ
                     FGWRIELVRALYEAGRPDEAAAEIRRLQAAGLSSIRDFDSLAPHYADVWRAGEKLHAW
                     RFFQNALKRRPGDVATLNLAAWLLATDDAPPAPPEEAVGLAQRAVELSGGPDPVLLDT
                     LAAALAAAGRYDEAERAAGEALGLAQARGMSGLVLRIGPRRALYAEGKAWREPPADGL
                     "
     gene            16618..17556
                     /locus_tag="PK634_07020"
     CDS             16618..17556
                     /locus_tag="PK634_07020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97524.1"
                     /translation="MPQELKIVEGGCPLCGSRNGELRLAGLRDVENRVPGEYAISRCR
                     DCQLHYLSTRPDEASLPGCYDTNYHVRFDRAVSPLMRGLFNARYALRMRRLLRHQGGR
                     PLTSLLEIGCGDGNLLAYLEKAFPAPVELVGVELDARHIRLPEGSRIRLHEADFDQLD
                     LGRQFDVVVMYHVLEHLVRPVETLRRIRSLLKPGGLLLFQVPNWNTCWRSLFPRHWNG
                     LQIPRHQFFLDPPRLADLCARGGFRLEAMSGLMDPGDFAVSVCNWITERLHLKTLPRR
                     AWFYLPAVILGAGLVAMTNLVTGQSGEMEAIARPAG"
     gene            17606..19780
                     /locus_tag="PK634_07025"
     CDS             17606..19780
                     /locus_tag="PK634_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97525.1"
                     /translation="MKNAAVRWIHVPLLALAVFLVYGRTLDAPFLLDDNYSIVGNPAL
                     RRLWPLTAALNPPPVNMTFCTRPFIHLTMCADYARSGLAPLGYHRTNLLFHLAASLAL
                     FGLARRTLARAGVEEKASRALGLAIALLWAVHPLNTTAVNYLSQRGELGVGLFLFLTL
                     YGLNRATERRSPGPAGSARAAGWLAAAVFFCLLGMGSKENMAAAPFLAMAYDRIFLGA
                     SWREVFRRRGFFHLALLLTLLWPVARILVYTPHVPKTGFDTDIYWRYPLTQAWGLARM
                     LRLAAWPHPLIFDYGNQLVNHAGQVWIPLLFMAALAGLTAWALFRHPKLAFPALCFFA
                     LLAPSSSFFPVTGQPIAEHRLYAALAGPLALAALGVWRLTGRRAGLFLPVAGTWVVAL
                     GIAAGCRNSLYTNRVVLWSDTVAKRPASERAWNSLGQAFNDEGRHEEALKAYERALAL
                     EPTSLVHVNAGITLNSLGRSEEALRQYREALRLDPSNADAENRLGIALASLGRQEEAV
                     EAQRRALALRPDHALAHLYLAESLAALGRLDEALPHYREAIRINPTLLEPHRGLAVAL
                     LAQGRTKEARSALLDGARLCGAPAQAYFDFSCALIDKGFAEEGLDMARRHLNLEPWHA
                     VSLNNAAWILATSTNAALRDGEEAVQLARRAVDRSGKTVPAIWATLAAAQAEAGQFQD
                     AVASAKQALELAREQGATNLAAPYEARLDIYRKGQPWRDNGN"
     gene            19759..>21930
                     /locus_tag="PK634_07030"
     CDS             19759..>21930
                     /locus_tag="PK634_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97526.1"
                     /translation="MARQRELKQKASPRAPAPAARKWMPPAWMAALALGLAVAAVYRG
                     TLNVSFLMDDGYNIVENPAIRRLWPPSAVLNPPTVEMTFYSRPFINLTMCVDYALSGL
                     DPRGYHRTNLLFHLAAVLALFGLVRRALRRAGVEEPAARGLGFAAALLWAVHPLTTAA
                     VNYLSQRGELGVGLFLFLLLYGLDRAAGDPADPTAPSPRVARAWLAAAVFSCLLGMGS
                     KESMAAAPLLAMTYDRIFLATSWKEMFRRRGLFYLALALTLLWPISRHFPVSSHIPKG
                     GFTPDTFWRYPLTQAWGLARLLGLSVWPAALVFDYGEGLVRGPAEVWPQLLLIFALLA
                     LTGWALRRHPRAGFAALCFWALLAPSSSFFPVTGQPVAEHRMYAPLAALVALAATGLW
                     RLARRHERGRLFAVAVLVLALALGAAGYRRTALHASRVALWQDTVAKRPGSERGWNSL
                     GQALQDERRFGDALAAYEKAIAIKPTALGYVNAGVALHSMGRYEDAARYARQALELDP
                     SSELAESRLGIALASLGRHEEAIAAERRALELKPDSAIAHLYLAKSLAGLQRYDEALP
                     HYREAIRFQPDQLEPYAGLAVALFRAGRPGEARQVMRDVVRASRVPGLVYFELSRRLI
                     EEGLEGDGLEMARRHLDLEPGHIGSLNNAAWLLATSTNAALRNGAEAVRFARRAVEQS
                     AQPHPALLATLAAAQAEAGQFPEAVATTGRALDLARERGDTN"
BASE COUNT         3176 a         7807 c         7498 g         3449 t
ORIGIN      
        1 aacctggtca cgggcgattg ggcggcggtc gcgggccaga ccaacctgcc gggcgtcgcc
       61 gggttcatgt actacacaaa cgcgttcgcc gacccggcgc ggcacttccg gccgacggtg
      121 acgcggacga actggtgagg aaaatcatga tgccgtgcat gcgcagcccc ggggcagggc
      181 gcgctcgcgc cgtgagaggg gcgttgcccc caacgggggc tcacgcccga gcgcgccgcg
      241 gcggcttggg ccaagccgcc ctaccccttg tattcgtttc cctcatcttg ccactccacg
      301 ccgcgacgaa ctacgtgaac gtcgccaacc ccacgcccgt ggcgccctac accaactggg
      361 cgatggcctc gaccaacatc cagctcgcga tcaaccgggc ggcggcgggc gggctggtcc
      421 tcgtgaccaa cggcacgtac accggcgccc gcaagtccgt ttacccgtac ggttacacca
      481 tgctcgccgt caccaacggg gtaatcctgc gaagcgtgaa cggacccgag gcgaccacga
      541 tcaacggcct gggcgacaga cggggcgcaa tcatcagcaa cgccacggtc gagggcttca
      601 ccttcaccaa ctgcgtcggc gacgggatac tggcctacaa gggcgggggg gcggccctgg
      661 tcggcggctg ggggcggttg aacaactgca tcctgaccgg caaccggggc gtcaattacg
      721 gcggcagcgc cgcggtcttc tccctcggca tcatcagcaa ctgcgtcatc cgcggcaacc
      781 gagcgaccac ggcgggcggc ggcatctgca tgtacgaggg cgggctggtc gtggattgcg
      841 tcatcaccaa caatttcgtc ggcggcgccg ccggcgcgcg gggtggcggg atcgcctgcg
      901 agatcggcgg ggtggtcagc aattgccaga tcgtgaacaa tctgtcctgg atgcagggcg
      961 gcggcatttc gatcttcacg aacgggacgg cggccaactg cctcatcgcc ggcaataccg
     1021 ccaataccaa caacctcgag ggcgagggcg gcggggccta cctgcacatg gggggcctcc
     1081 tgcgaaactg cgtgatctcc aacaatttcg gcctctggca gggcggcgcg gtgaagttct
     1141 attacatggg cggcgtggtc cgcggctgcc tgatccgcga caactccacg ttgagctggg
     1201 gcggcgcgat gatgatgcgc tccggcgggc gcgccgagag ctgcacgatg gtccacaaca
     1261 ccggcggcga gtgcggcggc atctatttct acgcgatggg cggcacgagc ctgaactcca
     1321 tcgtctacca caacaccggc ggcgacctga ccaacgccta cggcggctcg gtgctctaca
     1381 gctgcgtgcc gaacaccggc ttcggcgcgg gcacgatcac gaacgacccc gcgttcgtgg
     1441 acctcgcggg cggcgatttc cggctctcga ccaactcgcc ctgcatcaac cgcgccacga
     1501 accaggcctg gatgtccggc gcgcaggacc tggcgttcgc cggccgcgtc cagaacgaca
     1561 tcgccgacat cggcgcgctg gaatcgtcct tctggagccg ctacgaagac gcggacgccg
     1621 acgggctctc cgacggcgac gaggccccga tgggcacgga ccggctcgac cccgactccg
     1681 acgacgactc gatgaacgac ggcgacgagg tctacgccgg gagccacccg accaacgcct
     1741 cctccttctt ccaggtcgag acgccggacg aggcgccggg cgaattcccc gtcatccgct
     1801 gggacagcgt cgcgggccgg acgtaccggc tcgagcgcac caccaacctc tcttcgagcg
     1861 cgttcagtat tttccagtcc ggcatccccg ccgacccgcc ggtgaacacg cacaccgacg
     1921 ccaccgccgc cgcggaaggc ccgtggttct accgggtcgt ggtggagtga aaaaaagcgc
     1981 ggcgccgttt ccggcgccgc gccactttaa tcagcacact ggatccggca acgggcacag
     2041 ccttaagcaa tccgaatgaa acctggagaa acgactttcg ccgccgcttc ttcggccgta
     2101 gaatgccggg ccatgcagac gaggctgacc agagggaacg cgatgctcct tctcgccgtg
     2161 gcggtgatcg cggtctatgc caatggtatc caggggcctt tcgtcctgga tgattcctat
     2221 agcatcgtgg aaaacccggc ggtccgcgcc ctctggccgc cctggatggc cctgaacccg
     2281 ccgcccgtca acatgacctt ctatgcgcgg ccggtgatca acctcacggc ctgcgtggac
     2341 tacgcgatct ggggcctgaa ccccatgggc ttccgcctga cgaacgtcat ttttcacctg
     2401 ctggccacgc tcgccttttt cagcctggtc cggcgcacgc tcgcgaccgt cggccgatcg
     2461 gacggcgagg cggtgttcat cggattcgcc gccgccctgc tctgggcggc gcaccccctc
     2521 ctgaccgccg cggtgaacta tccctcgcag cggggcgaac tggcggtggg cctgttttta
     2581 ttcctgatgt tatacgccct gaaccgggcg gccctggccg actccacctc cagccgatcg
     2641 tggctggcgt tgtccgttct gctgtgcctg ctgggcatgg gcagcaagga gacgatggcc
     2701 gccgcgccgt tcctggcgct ggcgtacgac cgggtgttcc ttgccggctc gtggcgcgag
     2761 gcgctgcgcc ggcgatgggg cttctacctg ctgctcgcgc tgacctggat atggccgctc
     2821 tggaaacacc ttaccttctc gccgcaggcc cagggcgacg tcatgaatcc gacgacgtgg
     2881 cacgcctacc tgctgacaca gacgtggggc ctgaaccgca tgatccggct gatgctgtgg
     2941 ccgcatccgc tgatcttcga ctacggcctc ggcatggcgc tccgcctcgc cgacgtccgc
     3001 gccgccgcca tcgggcttgt cctgctcatg ggcttcggag cctggagctg gttccgcttc
     3061 ccccgcgtcg ggttcgcggc cctctgtttc ttcgcggtcc ttgcgcccgc gagccttttc
     3121 cccgtgacgg gccagcccat cgcggagcac cgcatgtacg tcccgctggc gccggcgatg
     3181 ctgctgctgg tcctcggggc cgaggccctg gggcgggacc gccggtggaa cgcgcggcat
     3241 gcggggctcg ccctggcggc cgccgccgtg atcctcggcg gcttgaccgc gcatcgcaac
     3301 acgcggttcc ggaccgccgc ggcgctgtgg accgacacga tcgccaagtg gcccggcaac
     3361 gcgcgggcct ggtacaaccg gggctactat taccaggggc agggacagac cgacgccgcg
     3421 ctggccgatt acgcccggag cctggagttg aacccgcgct acgtcaaccc gctggtcaac
     3481 cgcgccaaga tccatttcga tcgccgggac cttcccgcgg cggcggccga cctcgacgcc
     3541 gcgctggcca tcatgccgga ctggccgagc atcctgacgc ggcgcgcgat gatccgccgc
     3601 ctgctcgggc ggcacgagga ggccgcggcg gacctcgatc acgccgcgcg gctggagccg
     3661 gagaacgcgg tgttcatcaa cgagctggcc tgggtcctgg ccacggcgcc ggacgacgcg
     3721 gtgcgggacg cgccccgggc cctggagctg gcgacccgcg ccgccgcgct gacggaccgc
     3781 gattaccacg cgctcgacac cctcgccgcc gcccgggcgg cgaacgggca gttcgaggcg
     3841 gcgcaggcgc tcgcccggga ggcggtcgcg caggcgcagc agcagggcga cccggccgcg
     3901 gcgcggcgga tcggcgagcg gctggcccgc tacgagcagg gccttgcctg gcgggaatag
     3961 cgtctttttt ccgggcgttt cacggcgcgg cgtgctataa gaaacccatg tttcggcgga
     4021 cgacagaagc ggcccctccg cgccgggagc cccggctttc atgacgacca cgctcttcat
     4081 ccccacgctg aacgagatcg agtccatgcg ccagatcctg ccgcgcatcc ggcgggaatg
     4141 ggtggaccag atcctggtcg tggacttgtc caccgacggc acggcggaat acgcgcgcgg
     4201 gcagggttgc gaggtggtgc ggcagcaggt caaggggctg cggcacgcct accgcgaggg
     4261 ctttccccac gtgcgcggcg acgtcgtgat cacgttcagc ccggacggca attcgatccc
     4321 cgaggtcatc ccggcgctga tcgcgaagat gaaggaaggc ttcgacatgg tcatcgcctc
     4381 gcgctaccgg cccccggcga agagcgcgga cgacgactgg atgacgggtt tcgggaactg
     4441 gctgttcacg cgcgccatca acctgctcca cgggcatgcg tggggccgcc cgtacacgga
     4501 cgccctcgtc atgttccgcg cctaccggac ctcgctgtac cgggagctcg agctggacct
     4561 cgacgacgga tacgcccccg aacgctggtt ccggaccatc atgggcgtgg agccgctgtt
     4621 gtcggtgcga gcggccaagc gccggctgaa gatcggggag gtcccggcgg acgaaccggc
     4681 gcggatcggc ggcacgcgga agctgcaact ggtccgctgg ccctgcgcgt acatgagcca
     4741 ggtgatcagg gagacctggc gctggcgcaa gaaagcggga ccgccggcat gaagaaacgc
     4801 cccaccagcc ggcgccgaag ccgggcaaag aaaccggcgg accgcgtggt gctgtcgcgc
     4861 gccgagtacg aggcgctgtt gcgcgatgcg cgcgccaagg cggtctacga cgagatcgcc
     4921 gcctacctgc tgaacggcat cgagacgctc ttgaatgacg agtctccgcc cgccccatga
     4981 acatcagcct cgtatatccg cagtggacgg aggagtacgg ccagatcgcg cacttcgcca
     5041 aaaaggcggg caagtggccg cccctgaacc tcgcctacct ggcggcgatc gcggaggccg
     5101 ccggccaccg ggtccgcatc accgacgccg aggccgaggc gctgcccatg gagacgaccg
     5161 tggaccgggt gctggcggaa aagcccgacc tcgtcggcgt cacggcgacg acgcccttct
     5221 accacatcgc ctgcgacctg gcggcgcaga tccggaagcg ctcggcggcg cccatcgcga
     5281 tcggcgggcc gcacgtgacg gtgctgcgcg aggcggcgtt cccgcgggag atggacttcg
     5341 ccttcatcgg cgaggccgac cggacgtggc cggccttcct ggagcgcttc gccgacgggc
     5401 gcgaccccgg cgacgtgccg gggctgctct atcgccagga cggcaaggtc cggtacaccg
     5461 gcccggccct tccggtggag gacctgaacg ccctgcccgt gcccgcgcgg cacctgctga
     5521 aggcggaccg ctatttcctc gggacgctgg agggcgccaa gcggttcacg tccatcatga
     5581 ccgtgcgcgg gtgcccctat cgctgcatct tctgcagcac gaaggtgttc gggcgcgata
     5641 cgcggcgacg cgacccgccg ctggttctcg acgagattcg cgattgcgtc gagcgcttcg
     5701 ggatcgagca tttcatgttc atggacgaca cgctcacgct gcaccgcgag cacatccgcg
     5761 ccatctgccg cctgctgatc gaggcgaggc tgaacatcac attcgagggc agcacgcggg
     5821 ccaacctggt ggacgaggag acggtgcggc tgatgcggaa ggccgggctg atccggctgt
     5881 cgttcggcct ggagtcggtg gacccgggca tccgcaagac gatgcgcaag caggtgccgc
     5941 tggagagtta catccaggcc aaccgcctga cccggaagca cgggatcgag acgctgaact
     6001 cgtgcatgat cggcctgccg ggcgagacgc gcgagacgat ccgcgccacg ctgcggttcc
     6061 tgcgcgaatc gcgcgagatc gagcaggcca acatcagcat tgccgtgccc tatcccggca
     6121 ccgagctgta cgagatggcc cggcgcgggg agatgggcct gcggctggag accgaagact
     6181 tctcgcagta ccgccgctac aacgccgccg tgatgacggt cggcgacttg agcccggccg
     6241 acctcgtcga gattcagaac gaggccttcg ccagcattta cctggccccg tggcgctggc
     6301 gcccgatgat cgccaagtcc ggcgccatgg gggccgtcct gaccgcccgc cggctggcgc
     6361 gagccctgaa ggagggccgc tcggacttcc tgaccgaccg tcagctgggg atctccgccg
     6421 ccgccccatc cctcccgccc tcggcgccgg acgccgagcc gggcgacccg gacagttccg
     6481 agctggtcca ttgagggccg cccgcggccc cgttccaggc ggtttgattt ctctgttcaa
     6541 cgacccgtgg tttaggatag agagtgacgg tttacggcgg gcccccgcgt attcgggcaa
     6601 agccgcggcc gggcgatgac aacgaccttc aagagcaagc gctacgacct cgcggtggac
     6661 accatcaccg aggccgacat ccgcgaactg atcgactggc tgcgcgccta tccccgcctg
     6721 acgatggcgc acgtgacgcg ggaattcgag aaggcctggt cggagtggct gggcgtccgg
     6781 tactcggtgt tctgcaactc gggttcgtcg gcgaacctgc tgatgtacgc ggccctggac
     6841 agcacggggc ggtcgcggaa ccgcgggatc gtcgtgcccg cggtgggctg gagcaccacg
     6901 gtcgggccga gcctgcagct cgggtggaag ccggtcatgt gcgaggccga ccccctccgc
     6961 tacagcctgg acgcgcgcgc gctggagcgc ctgctccgcg agcatcgccc ggacaacgtg
     7021 atcctggtcc acgtgctcgg cacgcccgcg gacatggagc cgatcctggc gctgcaggaa
     7081 aagtacaagt tcaacctgat ggaggactgc tgcgcggcgc acggcgcgcg cttccggggg
     7141 cgcaaggtcg ggacgttcgg ggcgatgtcg tcgttctcct ttttctacgg gcaccacatg
     7201 tcgacgatcg agggcggcat ggtctcgacc aacgacgagg agctgtacca ccacctgctg
     7261 atgctgcgca gccacggctg gtgcaaggac ctgccgccgg agaagtaccg cgagctcatg
     7321 gccgagcacg gcgtcgactc gttccactgc ccgttcacgt tcatcctgcc ggggttcaac
     7381 ctgcgtccgc tggacctgac cgcgcgcatc gggttgagcc agctgccgcg gctcgacgag
     7441 acggtgcggc gccgcgcgga gaaccaccgc cgctaccagc agcggctcga gggcgcggtg
     7501 gaattcgccc gcgtgggcga ggcggacctg atcagcagca tctccttcgg cggcgtggcg
     7561 gcgtcgaacg cccagcggaa ggtcatcgtg tccgccctgg tgaagaacga gatcgacacg
     7621 cgcctcttct cggccggcaa cctcgggcgc catcccttct ggacgaagcg gcacggcgtc
     7681 ttctcggccc cggtggccga ccgcatccac gatggcggct tcttcctgcc caacaaccag
     7741 tccctggatg cgggcgacat cgatttcgtg agcgacgtgg tgatcaaggc cgtcaggggc
     7801 tagaccggaa ggcgggccgc atggcgtcgg acattctgtt catccatccc ggcaaccaga
     7861 aagccatcta ccaggacctc gcccgtgagt tcacggccgt tgatcccccg atctgggcgc
     7921 tgctgctggc gggcgcgttg cggcgcaagg gcctcgatgt cgccatccat gacgcgcaca
     7981 tccagggctg ggacgcggac atcgcgcgcg acgcgctgcg gcggcacgag ccgcgcctcg
     8041 ccgtgatcct ggtgtacggg caccatccct cggcctccac gcagaccatg cccgccgcgc
     8101 gcgccgcgct ggccgcgctc aaggaggccg cgccggacct gcccgtcgcg ctgggcggca
     8161 cgcacccgtc cgcgctgccc gagcagacgc tgcgcgagga gcccgcggat ttcatcgtgc
     8221 agggcgaggg cctgcacacc ctcctcggcc tcgccgcgca ctaccacggg aagcagcgcc
     8281 cggaggacat cccgggcctg tggcggcgga cggaaaacgg gccggccttc aacggcccgg
     8341 cgcccgtggc gcgcaagctg gacgaggagc tgccggagta cgcgtgggac ctgctgccgg
     8401 gcctccataa ctaccgcgcg cacaactggc attgcttcca ggatttcgcc cgctcgaagg
     8461 cgcccgactt ctccgacgtg cgctcgccgt atgcctcgat ctactcgtcg ctcggctgcc
     8521 cctacgattg cgactactgc tgcatccaca cgctcaccgg cggcaagccg ggcctgcggc
     8581 cgtggccgat cgagcacgtc gtggaggtcg ccgcgcacct cgcgcagaaa cacggcgtgc
     8641 ggaacatccg gctcgccgac gagctgttcg tcctgaaccc cgcgcgggtg gagcggttct
     8701 gcgaccggat gatcgaacgc gggctggaca tgaacttctg gtcctacgcg cgggtggaca
     8761 cgatccccga ggcgctgctg ccgaagatga agaaggccgg gctcaactgg ctgtgcctcg
     8821 gcatcgagtc ggccaacgag cgggtgcgcg cgtcggtgaa caagatgctg cggcgcgaca
     8881 tcgtgtccac cgtgcggaag atccaggacc acggcatctg catcatcggc aactacatgt
     8941 tcggcctgcc ggaggaggac gccggcacga tgcaggagac gctcgacctc gcgctggagc
     9001 tcaactgcga gttcgtcaac ttctacaccg tcatggccta tcccggctcg cggctgtacg
     9061 cgcaggcccg cgcggcgggg accgtgccgg accgctggga ggccttctcc cagcacagct
     9121 tctacacgca gcccctgccc acgcggcgcc tgaccggcgc cgaggtgctg gcgttccgcg
     9181 accgggcctt tgagacctat ttccgcgacg cgcgctacca ggccatgatc cgccggaagt
     9241 tcgggcccgc cgtttgcgcg cacctcgaaa agatgctagg gttcaagatc aggcggaagc
     9301 tgctggagtc cgcttcggcg tagggagatc gcgcgaaagc cgggaggcat gctatggcgg
     9361 cgacactgaa ggccaaggcc tacgaactga agaagaccat gctggagacc tgcatccgcg
     9421 cgggcaccgg gcacgtcacc agctcgctct cctgcgcgga aatcctgacg accctgttcc
     9481 acggcggcgc gctgcggttc gacccgaagc gccccgcctg ggaggaccgc gaccggttca
     9541 tcttgagcaa ggggcaggcc agcccgatgc tctacaccgt cctcggcgac ctgggctact
     9601 tcccgaagga ggacctgcag aagttcgcgc aggcgggcgg gccgttcggc gtgcacctgc
     9661 agaagagcgt gccgggcgcg gagatcacct gcggctcgct cggccacggg ttcggcctcg
     9721 ccgcgggcat ggcgctcgcc gcgcggatgg accgccggaa ccacctggtg ttcaccctgc
     9781 tcggcgacgg ggagtgctac gagggctcga tctgggagac cgcgctgttc gccgggcacc
     9841 accgcctgaa caacctcgtc gccatcgtgg accgcaatta ccagtgcgtg ctcgacttca
     9901 ccgagaactt cctcgcgctc gagccgatgg aggagaaatg ggaggcgttc ggctggcgcg
     9961 tgaagcgcgt ggacgggcat tcgttcgacg acctgctcga cgcgctgaag ggggtccggt
    10021 cgcgccgctc cgtgcagccg ctggtgatcc tcgccgacac ggtcaaggga gccggcgtgg
    10081 gattcatggc ctgcaatccc atctggcacg gcgccgcgcc gcgcggcgac gaggcccagc
    10141 aggccctgcg cgagctcgaa aggagcgccc gcgatgaata acgtcagcca gcgcgacgcc
    10201 ttctggaccc gtctttacga ggtcgcccgg caggaccgcc gggtgatcgc cgtcgccgcg
    10261 gacatgggcg cgcccgccct cgaccagttc cggcgcgacc tgcccgggca gttcgtcaac
    10321 gcgggcatcg ccgagcagaa cggcctgctc gtcgccgccg gcctggccct ggagggcaag
    10381 cacccgttcc tctacgccat cgccccgttc gccacgttcc gcgtcctcga gctgatccgc
    10441 gtcgagaaca gcatcatgga catccccgtg acgatcgtcg gcgtcggcgc gggcttcggt
    10501 tacgaggatt ccggcccgac ccaccacatc ctcgaggaca tcgccgcgct gcgggcgttc
    10561 ccgcacgtca cgatccacag cgtcagcgac tccacgatgg ccgccgccgt cgcggagcat
    10621 tcctgcggga tgaagcacgc gaactacgtc cggctcgacc gcgccgtcct gccggagctc
    10681 taccaggccg cgccggacct cgcgaagggc ttcgccgtcc tgcggccgag caaggccggg
    10741 tggctgatcg ggaccggcta catgacgcac aaggcgctgc gcctcgcgaa ccggctcgcc
    10801 ggcgaggggc tggacctcgg cgtgatggac ctcttcgcca tgcccttcga cgagaaggcc
    10861 ttcgtcgcgg cgctcggcaa ggcgccccgc ctgttcacgc tggaggagca tttcctggcc
    10921 ggcggcatgg gctcgctggt cctcgagacg ctcaatcgtc acggcctggc gatccccgtg
    10981 aagcggttcg ggctggagat ggacaagggc tactcctacg tgtacggcgg ccgcgaccgc
    11041 atccttgccg gttacgaact gtccgaggac gacctggcca aggccatccg gaactggaag
    11101 taacaggagg caacggagag agcggagaaa gctgccgcct ccgttcattc cgttggctcc
    11161 tgttcagcct gttcagtttt ttcttttcca atgtctggga aaagggatta gccttttttc
    11221 caatgtctgg aaacgcctcc agcgccccgc cgccggcccg gctccggtgg ggccccgccc
    11281 tgctgatatt cctgtccgtc ttcgtcgcct tcggcggcgc gctgcggaat gatttcgtgc
    11341 ggtttgacga cgatctctac gtcaccggca atccgcacgt ccgcgcgggg ttgacgcggg
    11401 agggcgtccg ctgggccttc acgtccatgc acgacgccaa ctggttcccg ctgaccctgg
    11461 cctcccacat gctcgacgtc tcgctcttcg gcctgaaccc cgccggccac catttcacca
    11521 gcctgctgct gcacgccctg aacggcgcgc tgctgtacgg cctgctgcgg cggctgaccg
    11581 gccgggcctg gcccgcggct ttcgcggcgg cggtatgggc cctgcatccg ctgcgcgtgg
    11641 agtccgtggc ctgggtgtcc gagcgcaagg acgtgctctg cgccttgttc gggctgctga
    11701 cgctgctggc ctacgagcgg tacgcgcgcc ggcccacagc cgggcgctac ctggcgacgg
    11761 gtctttattt tgcgctcgga ctgatggcca agccgatgct cgtcacgctg ccgttcgtca
    11821 tgctgctcct ggactactgg cccctcggac gcgggccggc cgagggatgg cggcacctgg
    11881 cgctggaaaa actcccgctg ttggcaatcg cggcgaccgc gtgcgtcgtg accttcctcg
    11941 tccaacgcgc gggcggggcc ctgaaaacga tcgatcagtt tgcgctggcc gtgcgggccg
    12001 gcaatgccgt ggcggcctat gccgagtaca tccgcctgct gctctggccg gcgaatctcg
    12061 ccgtcttcta tccgcatccc ggcgtcgttc cgccgtggtc ccggctgctc ccggcgatgg
    12121 ccgtcctgct gggcttcacg gcgctcgcgt tgcgcctcgg gcgacggcag ccgcacctgc
    12181 ccgtcggctg gttctggtat ctcggcatgc tgattccggt catcggcctc gtccaggtcg
    12241 gcatccagtc gcatgcggac cgctacacgt atcttccgca tatcgggctg ctgatcctgc
    12301 tggcctggag cgcggaccgg tggctggggg aaaggacgac ggcccgaatg gccgcgttcg
    12361 cggtcctgct cccgctggcc tgggccgccc accggcagac cgcgacctgg cgcgacagcg
    12421 aaagcctgtt ccttcgcgcc atcgccgtca ccggcccgaa tgccgtcgcc cggaacaacc
    12481 tgggggctca ctacgccacg cagggccgcc gcgccgaggc ggaggaacac ctgcggcgga
    12541 gcctggagat ccagccggga tatgccgacg cgcagttcaa cctggccaac ctgctcctcg
    12601 aagcgggccg gcgtcccgag gcgatcgccg agtatcgcgc cacgctggag tacaggcccc
    12661 atcacctccc gtcgctgaac aacctcgcct ggctgctggc caccgacccg gattccaatg
    12721 cggaggaccg cgccgaatcc ctcgttctcg ccgagagggc gatcggcccg acccgctctc
    12781 ccgacgccgg ccttctcgac accctcgccg ccgcgctggc cgccaacggc cggttcgagg
    12841 aggccgcgag ggcggcggcc caagccctcg acctggccag ggcagcgggc caggaggacc
    12901 tcgcccggcg actggaacgg cggcttgccg cctaccgcga gggaaggatc cggcgggaat
    12961 gaaaggcgac gttctcgcgt gcacgatcct ggcgctcgcg ccgctggtca gcgcggccgc
    13021 gctggcgggc ctgatccgct tctcccgccg cgccgacggc cggcgccgag atgcggtcac
    13081 ctggctggcg cggatcatga tcccggtttt cctggtgtcc gccggactgg ccgccggcga
    13141 agcctacttc cggtttttcc acgacgcgtc tgattcctac gggctctccc gcgtcgcccg
    13201 gcgctggttc gagcggcact ggcggctgaa ccgctcgggc ttccgcgacg acgcggaata
    13261 cacgattcag tgcgctcccg ggaggcgccg gatcacgttc gtcggcgatt ccttcacggc
    13321 gggccacggc ctggcgaacg tcagggaccg tttcgcgaac cggctgccgg acggaagggc
    13381 atacgaggtt cacgtcctgg ccaaggccgg ttacgacacc ggcgagatgc tggaactgct
    13441 gcaggacctg cgggcgcagg gctacgggat ggacgaggtg gtgctggtgt atgtgcccaa
    13501 cgacatttcc gacatcgcgc ccgggcacgc ccgcatgaac cggcggatct ttgcccggcc
    13561 ggcgggattc tgggtaaacc acagcctgct gttcaacacg ctgtaccatc gcgggagggc
    13621 gaggacggac ccggacgtgg cggggtacta ccggtacgtg gagaaggcct actcgggccc
    13681 aacgtgggag gaacagcaga ggcggcttct ggccctgcga gacctggcgg aaagtcaggg
    13741 cggccggttc cgcgtcgtga ccttcccctt cgtgcagggc ctgggggccg gcaacgagtt
    13801 ccgcgctgtt catgcaaagc tcgccacctt ctggacgggg cagggagttc cgcatctcga
    13861 cctactgggg gtgttcgagt ccgcgccagg ccgccgttgg accgtcaatc ggtacgacgc
    13921 gcatccgaac gccgccgcgc atgcgctggc ggcggaggcg ataggcgctt tccttcagac
    13981 cggcgcgcgc cccgccgctc cggccccatc ggccggcccc gcgggaccgc tgttacagcc
    14041 gatcctgaca accaaggagc gggcgcagcg cggcgccgcc gagttagcgc aaagcaggtt
    14101 tgcggaggct gtggcagact ggcggatcgc gctggcggcg cggccggatt ccgcggagat
    14161 tctgaacaac ctggcgtgga tcctggctac ctgccgggac ccggccttgc gcgacggcgc
    14221 ggaagcgatt ctcctggcgg aacgggcatg cgcgctcgcc acgccggacg aggccgggtt
    14281 ccgggacacc ctggccgccg cgtacgccga ggccgggcgg ttcgacgagg cgatccgcgc
    14341 cgcggagctg gcgcgggaca tcgcgcgcca cgccggcgac gaagcgctgg ccgccaggac
    14401 cgagcaacga ctggtaggct accggcgcgg acggccgtgg cgggattgac cgcgcacacg
    14461 ctccggatgc cgcctctttt gttttccaat cattggaaat ttcgactact ttttccccat
    14521 gtttggaaac ccgacagcct cgcggcgggc gcccgccatg ttggccctgc tgctggcgct
    14581 gctgacgttc gcgctcttcc ggcccgcggc acgctacggc tacgtcaagc tggacgacga
    14641 ccagtacgtc gcgagcaacc tccgcgtgac cacgggcctg acggcggaca acgtccggtg
    14701 ggcctttcac acggtctacg aggactggtg gctccccctg ctctggatgt cgttcatggc
    14761 ggacagcgag gtctacggtc ccgtgcccgc cgggtaccac accaccaaca tcctgctcca
    14821 cgcgctcaac gcggggctgt tgtgctggat cctgttccgg ctcacgggat cccgctggcg
    14881 aagtttcttc tgcgccgccc tgttcgccgt gcacccgctg cgcgtggaat cggtggcctg
    14941 gatcaccgcg cgcaaggacg tgctgagcgg gctcttcttc ctgctcggcc tgctggccta
    15001 cgttcgatac gtcgagcggc cgggcgcggc gcgattcgcc gtcctcgcgg cgctgatgct
    15061 gatggggctc ctgtcgaagg ccatcgtgct ggtcctgccc ttcgtcctgc tgctgctgga
    15121 ctactggccg ctgcggcggg ccggcgaccc gcgggatccc gccgggctga aaacctggcg
    15181 cgggctcctc gcggaaaaaa ttccgctgtt cctgctggcc ggcgtgttca tcctgatcaa
    15241 cctgggcacg cacgtcgcgg gccgcggggc cgattcgaac ctgtccgcgg cgacgcggct
    15301 cggcctgatc ccgcccaatt actgggcgta tctcgccaag atctgctggc cggtccggct
    15361 cgttcttttc tatcccgagc atgacgtcgt gcgctggccg gtcaccctgg ccgccctggc
    15421 gggcctgctc ggcgtcacac tcctgctgct gcggtgccgc aaggccgcgc cgcacctggc
    15481 cgtgggctgg ctctggttcc tcgtcgcgct gtttcccgtg atccgcggcg tgcggctcgg
    15541 gctggcggcg tacgcggacc ggttcgccta cctgccgtcc atcgggctgg gcctggctct
    15601 cgtctgggga gtcgcggccc tcgcggaacg gcgtcgatcg ctcgccgcgg ccgccgcgct
    15661 ggcgggcctg ctgctgctcg gcgccggcgc atggaaaacc cgcgcgcaac ttccgttatg
    15721 ggcggactcg ttcaccatgt tcggccgcct ggtccggctc gagccggatt gcgacctggc
    15781 gaacaacagt ttcggcaagg cgctgatgga gcgggggcca ttggccgagg cgctgccgta
    15841 cttcaagaag gccgcggcgc tcaacccggc gggatcccgc ggcgtggtca actatgccga
    15901 cgccctgatc cggctcgggc gcgacgacga ggccatcgcc tggctgcagg acgcccgcgc
    15961 gaaggggtat cccgaggtga ccgaactgga cgtcctgctc ggcttcgcgt acctgaacac
    16021 cggccgcgcg gaggaggccg tgccgcgcct ccagcgggtc tcgcggaacc ggcccgagca
    16081 gttcggctgg cgcatcgaac tcgtccgggc cttgtacgag gccggccggc ccgacgaggc
    16141 cgccgcggag atccgccgcc tccaggccgc ggggctttct tccatccgcg atttcgacag
    16201 cctggccccc cactatgccg acgtgtggcg cgcgggggag aagctccacg cgtggcgttt
    16261 cttccagaac gcgctgaagc ggcgtcccgg cgacgtcgcg acgctgaacc tggcggcgtg
    16321 gctgctcgcg acggatgacg ccccgcccgc cccgccggag gaagcggtcg ggctggcgca
    16381 gcgggcggtg gagttgagcg gcgggccgga tcccgtgctg ctcgacaccc tggccgccgc
    16441 gctcgcggcg gcggggcgct acgacgaggc cgagcgcgcg gccggcgagg ccctgggact
    16501 cgcccaggcg cgcgggatga gcggcctggt cctgcgcatc gggccgcggc gcgccctgta
    16561 cgcagaagga aaagcctgga gggaaccccc ggcggacgga ttgtaaagga gcgcggcatg
    16621 ccacaggaac ttaaaatcgt cgagggcggt tgcccgctgt gcgggagccg gaacggcgaa
    16681 ctccggctcg cgggcctgcg ggacgtggag aaccgggtgc ccggcgagta cgccatttcg
    16741 cggtgccggg attgccagct gcactacctc tccacgcgac ccgacgaggc ctcgcttccc
    16801 ggctgttacg acaccaacta ccacgtccgc ttcgaccggg cggtgagccc cctgatgcgc
    16861 ggcctgttca acgcccggta cgccctgcgc atgcgccggc tgctccggca ccaaggcggg
    16921 cgcccgctca cgtccctcct cgagatcggc tgcggcgacg ggaacctcct ggcctacctg
    16981 gaaaaagcgt tcccggcccc ggtggaactc gtgggcgtcg aactcgacgc gcgccacatc
    17041 cgcctgcccg agggcagccg cattcgcctg cacgaggccg atttcgacca gctcgacctg
    17101 ggccggcagt tcgacgtggt ggtcatgtac cacgtcctgg agcacctggt gcgcccggtg
    17161 gaaacgctgc ggcgcatccg ctcgctgctg aaaccgggcg ggctcctgct gttccaggtg
    17221 cccaactgga atacctgctg gaggagcctt tttccccggc actggaacgg cctccagatc
    17281 ccgcgccacc agttcttcct ggacccgccg cggctggccg acctgtgcgc ccggggcggg
    17341 ttccgccttg aggccatgtc gggcctgatg gatcccggcg acttcgcggt gtcggtttgc
    17401 aactggatca ccgagcgcct ccatctcaag accctgcccc gccgcgcatg gttctacctg
    17461 ccggcggtga tcctgggcgc gggcctggtg gcgatgacca acctggtcac cggccagtcc
    17521 ggcgaaatgg aggccatcgc gcggcccgcg gggtaggacg ctgccgcgtt gccgtgggtt
    17581 ccgcgccgat gtatcatggt ccgcgatgaa aaacgcggcg gtccgttgga tccacgtccc
    17641 cctgctggcg ctggccgtgt tcctcgtcta cggccggacg ctcgacgccc cgttcctgct
    17701 ggacgacaac tacagcatcg tcggtaatcc cgccctccgc cggctgtggc cgctgacggc
    17761 ggcgctgaat cccccgccgg tgaacatgac cttctgcacg cggcccttca tccacctgac
    17821 catgtgcgcg gattatgccc gaagcgggct ggcgccgctc ggctaccacc ggaccaacct
    17881 gctgttccac ctggcggcct cgctggcgct gttcggcctc gcgcgccgca cgctcgcgcg
    17941 ggccggcgtc gaggaaaagg cctcgcgggc gctgggcctg gccatcgcgc tgctctgggc
    18001 cgtgcacccg ttgaacacga cggcggtgaa ttacctgtcc cagcgcggcg agctgggcgt
    18061 cggactgttc ctgttcctga ccctgtacgg cctcaaccgc gcgacggagc gccggtcgcc
    18121 cggcccggcc gggagcgcgc gggccgcggg ctggctcgcc gcagcggtgt tcttctgcct
    18181 cctgggcatg ggcagcaagg agaacatggc cgcggcgccc ttcctggcga tggcttacga
    18241 ccggatcttc ctgggcgcgt cgtggaggga ggtgttccgc cggcgcggct ttttccacct
    18301 ggccctcctg ctgaccctgc tctggccggt cgcccgcatc ctcgtctaca cgccgcatgt
    18361 cccgaagacg ggcttcgaca cggacatcta ctggcgctat cccctgaccc aggcctgggg
    18421 cctggcgcgg atgctgcggc tggcagcctg gccccatcct ctgattttcg actatggcaa
    18481 ccagcttgtg aaccacgcgg gccaggtctg gatcccgctg ctcttcatgg ccgcgctcgc
    18541 ggggctcacg gcatgggccc tgttccgcca tccgaaactg gccttccccg ccctgtgctt
    18601 cttcgccctg ctggccccct cctccagctt cttccccgtc acgggccagc cgatcgcgga
    18661 gcaccgcttg tacgcggcgc tcgccggccc gctggcgctg gccgcgctgg gcgtgtggcg
    18721 gctgacgggc cggcgggccg ggctcttcct gccggtcgcc gggacatggg tggtcgccct
    18781 gggtatcgcg gcgggctgcc gcaacagcct gtacacgaac cgggtcgtgc tgtggtccga
    18841 caccgtcgcc aagcggccgg ccagcgaacg cgcctggaac agcctcgggc aggcgttcaa
    18901 cgacgaagga cgccatgagg aggcgctgaa ggcctacgag cgggctctcg cgctggaacc
    18961 gacctcgctc gtccacgtga acgcggggat cacgctgaac tcgctgggcc gcagcgagga
    19021 agccctgcgc cagtaccgcg aggcgctgcg cctggatccc tccaacgcgg acgcggagaa
    19081 ccggctgggc atcgccctgg cctcgctcgg ccggcaggag gaggccgtcg aggcccagcg
    19141 gcgcgccctg gccctgcggc ccgaccatgc cctggcccac ctgtacctcg cggagagcct
    19201 cgcggccctg gggcgcctgg acgaggccct gccccattac cgcgaggcga tccggatcaa
    19261 cccgaccctc ctggagccgc accgcggcct ggccgtcgcg ctgctcgcgc aggggcgaac
    19321 gaaggaggcc cgctccgcgt tgctcgacgg cgcgcggctc tgcggggcgc cggcccaggc
    19381 gtatttcgat ttctcctgcg cgctgatcga caagggcttc gccgaggagg ggctggacat
    19441 ggcccggcgc cacctgaacc tggagccatg gcatgccgtt tcgctgaaca acgcggcctg
    19501 gattctggcc acgtccacca acgcggcgct gcgcgacggc gaggaagcgg tgcaactcgc
    19561 ccggcgcgcc gtggaccgct ccgggaagac cgtcccggcc atctgggcca cgctggcggc
    19621 cgcccaggcc gaggccgggc agttccagga cgccgtcgcc tccgcgaaac aggccttgga
    19681 actggcccgg gaacagggcg cgaccaacct ggccgccccg tacgaagccc gcctggatat
    19741 ctataggaag ggacagccgt ggcgagacaa cgggaactga agcagaaggc ctcgccgcgc
    19801 gcgccggcgc cagcggcgcg gaaatggatg ccacccgcct ggatggccgc gctggcgctc
    19861 ggcctggccg tcgcggccgt ctaccgcggc acgctgaacg tctccttcct catggatgac
    19921 ggctacaaca tcgtcgagaa cccggccatc cggcggctgt ggccgccgtc cgccgtcctg
    19981 aatccgccga ccgtggaaat gacgttctac tcgaggccgt tcatcaacct gaccatgtgc
    20041 gtggactatg cgctctcggg cctggatcct cgcgggtatc accggaccaa cctcctgttc
    20101 cacctggccg ccgtgttggc gctgttcggc ctcgtgcgcc gcgcgctccg gcgcgccggg
    20161 gtcgaggaac ccgccgcgcg ggggctggga ttcgccgccg cgctgctctg ggccgtgcac
    20221 ccgctgacga cggccgcggt gaactatctt tcccagcgcg gggaactggg cgtcgggctc
    20281 ttcctgttcc tgctgctgta cggcctggac cgggccgcgg gcgatccggc ggatccgaca
    20341 gccccgtcgc cgcgggtcgc ccgggcctgg ctggccgcgg ccgtcttctc ctgcctgctc
    20401 ggcatgggga gcaaggaatc catggccgcg gcgcccctgc tggccatgac ctatgaccgg
    20461 atcttcctgg ccacgtcgtg gaaggaaatg ttccgccggc gcgggctctt ctacctcgcc
    20521 ctcgcgctga ccctgctctg gccgatctcc cggcatttcc ccgtttcgtc gcacatcccc
    20581 aagggcgggt tcacgccgga cacgttctgg cgttatcccc tgacccaggc gtggggcctg
    20641 gcccgcctgc tggggctgtc cgtctggccc gcggcgctgg tctttgatta tggcgagggc
    20701 ctggtgcgcg ggccggccga ggtctggccg caactgctgc tcattttcgc cctgctcgcg
    20761 ctgacgggct gggcgttgcg tcgccacccg cgcgccgggt tcgcggccct gtgtttctgg
    20821 gccctcctgg cgccctcgtc cagcttcttc cccgtcacgg ggcagccggt tgccgaacac
    20881 cggatgtatg ctccgctggc ggcgctggtc gcgctggcgg cgaccgggct gtggaggctc
    20941 gcgcgccggc atgaacgcgg gcgcctgttt gcggtggccg tcctcgtgct ggccctcgcg
    21001 ctcggcgcgg ccggctatcg acgcaccgcg ctgcacgcgt cccgcgtggc cctttggcag
    21061 gacaccgtcg ccaagcggcc cggcagcgag cgcggatgga acagcctggg ccaggccctg
    21121 caggacgagc gccgtttcgg cgacgccctg gccgcctacg agaaggccat cgcgatcaag
    21181 cccacggccc tgggttacgt gaatgccggg gtcgccctcc actcgatggg ccgctacgag
    21241 gacgccgccc ggtacgcccg ccaagcgctg gaactggatc cgtcctccga gctggccgaa
    21301 agccgcctgg gcatcgccct cgcctcgctc ggccggcacg aagaggccat cgccgccgag
    21361 cggcgcgcgc tcgagctcaa gcccgactcg gccatcgccc atctctacct ggccaaaagc
    21421 ctggccggac ttcagcgcta cgacgaggcg ctgccgcact accgcgaggc gatccgcttc
    21481 cagccggacc agctcgaacc gtacgcggga ttggcggtgg ccttgttccg cgccggccgc
    21541 ccgggcgagg cgcgccaggt catgcgcgac gtcgtgcgcg ccagccgcgt ccccggcctg
    21601 gtctatttcg agctctccag gcggctgatc gaggagggtc tggaaggcga cgggctcgag
    21661 atggcccgcc ggcacctgga cctggaaccg ggccacatcg gctccctgaa caacgcggca
    21721 tggctcctcg ccacctccac caacgccgcc ctgcggaacg gcgcggaggc cgtgcggttc
    21781 gcgcggcgcg ccgtcgagca atccgcgcag ccccacccgg cgctcctggc cacgctcgcc
    21841 gcggcccagg ccgaggccgg gcagtttccg gaggccgtcg cgaccaccgg gcgcgccctg
    21901 gacctggcgc gggaacgggg cgacaccaac
//
LOCUS       DAOOTK010000046        21658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1663_length_21658_cov_6.295561, whole genome shotgun sequence.
ACCESSION   DAOOTK010000046 DAOOTK010000000
VERSION     DAOOTK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21658
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_21658_cov_6.295561"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="mvaD"
                     /locus_tag="PK634_07035"
     CDS             complement(<1..799)
                     /gene="mvaD"
                     /locus_tag="PK634_07035"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOW97527.1"
                     /translation="MATKRDIVGRILAGRGMKPGAAGCAFAPANIALVKYWGKRDEEL
                     NLPVTSSLSVSLGRLGSEVEIQPAGPADVVVLNGQELPPDSSFARRVSAYLDLFRPAG
                     AGFRVDARNTIPTAAGFASSASGFAALVRALDQLFGWRLETRELSILARLGSGSACRS
                     VYEGFVEWRAGEAADGMDSYAVPLAETWPDLRMGLVVISGEEKAVGSRAGMKRTVETS
                     PLYRAWPAKVAADLPEVRAAIRERDFHRLGRAAESNALAMHATMIAAW"
     gene            complement(820..1206)
                     /locus_tag="PK634_07040"
     CDS             complement(820..1206)
                     /locus_tag="PK634_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97528.1"
                     /translation="MRNKILAMLMSLWMGAGLVLVGCEDDGGGDDSPVDVTGTWTGEI
                     VKESGETGTGTLILVQNGTNVTGTDHDTWTANAAVQGSVDGRNVELTIMDDVGTHLSG
                     HVSGDRMDYTWTSGDGTSGSGTLTRQ"
     gene            complement(1240..2187)
                     /gene="mvk"
                     /locus_tag="PK634_07045"
     CDS             complement(1240..2187)
                     /gene="mvk"
                     /locus_tag="PK634_07045"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOW97529.1"
                     /translation="MLLQPCLVTASFQASAPGSLMLMGEHAVVHGRRALVAAVNRRIR
                     VRLEPRGDGQVRIASALGEVEMPLGRLRSPAPFRFVMAMLRRYEARLPSGFDLNIESG
                     FPSTVGLGSSAAVTVATCAALERWTRSRRPPPWDLFVASREVIREVQGLGSGADVAAS
                     VFGGILLYRAAPAQVQRLKRTIPLVVLYSGGKTPTPEVVRRVQRAMKAQPAFYGDIFD
                     LMDRGCAAGFQALRAGALKRFGELLDIQQGLMEAIGVSNARMAELVYALRGERGILGS
                     KISGSGLGDCVIGLGRSDRTDWPGPRIEAEISSEGVKVE"
     gene            2269..3078
                     /locus_tag="PK634_07050"
     CDS             2269..3078
                     /locus_tag="PK634_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOW97530.1"
                     /translation="MNWLTELHPVLQALLATLFTWGVTALGSAGVFFFKSVNRRVLDA
                     MLGFAAGVMIAASFWSLLAPSIELAAEMGMVKWWPPLAGFLAGALFLYGIDRILPHLH
                     LNLSLDQAEGIHTHWKRSILLILAITLHNIPEGLAVGVAFGAVAAGIESTSLGGAIAL
                     AVGIGLQNFPEGMAVSLPLRREGISRWKCFWYGQASGLVEPVAGVIGAAAVLMMRPLL
                     PYALAFAAGAMIFVVVEELIPESQSSEKTDVATLGAIAGFAVMMLLDVALG"
     gene            complement(3093..3470)
                     /locus_tag="PK634_07055"
     CDS             complement(3093..3470)
                     /locus_tag="PK634_07055"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOW97531.1"
                     /translation="MTSSLQRWIRIAEQEVERTRGRLPPSLRDRAAEIPVTCVPRPTR
                     AMQRDGVDPELLGLFVGEALPMTEAPGGDLPAQILLFIENLADYSDCDEDIFQEEVRT
                     TYLHELGHYLGLDEDELVERDLE"
     gene            complement(3467..4897)
                     /locus_tag="PK634_07060"
     CDS             complement(3467..4897)
                     /locus_tag="PK634_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW97532.1"
                     /translation="MLALINTNRMRPAIAPVGLDYAGGAARAAGIEVAWLDLGLADDP
                     SAAAKAFFKERSPRLVGLTLRNADDSFWPSAASFVEPLRDRVRALRRLTKAPIVLGGA
                     GFSIFASEILDFTGADYGIRGDGEAALPALYRAVSAGDSPDRVPGLVTPRGANPPAWD
                     GDGPLNVPTNRDVAENAAYFRLGGQIGLETKRGCPLACDFCADPIAKGARARCRPPAD
                     TADEAEALLRQGVDVFHLCDGEFNVPVDHARAVCEEWIRRRLGERVRWYAYLSVTPFD
                     AGLARAMRQAGCVGINFTGPAATPTMLRAYGVRHRVEDLAAAVRAARAAGITTMVDLM
                     LGGPGETPTTVRQAVDFLRILKPDCVGAALGVRLYPGLPLTRRIAAEGPLETNPAIRR
                     RYEGPVNLLWPTYYIAAALGRRPAAVVRDILAGDPRFFEPPPDEESGQKSYNYNDNAP
                     LVDAIAAGARGAYWDILRQVRQRSAT"
     gene            5024..6727
                     /locus_tag="PK634_07065"
     CDS             5024..6727
                     /locus_tag="PK634_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOW97533.1"
                     /translation="MQRRIALLVLCVSAARLQAASPAREAVSFRHTWDVGLGRSVFVV
                     GSHADLGDWSPESAVKLYWTSGNVWTGRVAVQAGAALEYKFIARTNSADQYCNGDNVE
                     WMAGTNWPAQVAAQPDAPYAGKTILYHSGWTNAGLVYRVGTNWFGAAMSRVGAGRNGG
                     EYLYQASGFGAAGEPIEFVPYGYSGGVQYWDHAPYGGYGDSNYYTTLDVFFLQDGHVF
                     NYRPPTNVSASRIIGTNVTSSWAPTIPSRGVRIYLPRGYDQNTWKRYPVLYMHDGTNV
                     FQPGGTLGCWNADLLADREISQGRMRESIIAAVDTTPKRDSEYCPAGDQYGPEPGTGD
                     QYANFLVHNVRPMVDTHYRTLNDFDNTLTAGSSMGGLISAYLGLATNVFGKIGAFSPA
                     FLVAPNFIAWMDAQDTRGVRMYMDDGTVDLDVDLWPDTWAAYDLLMQDGYVPNDDLLM
                     VVGCGQTHNEAAWASRLPRVYQYLLSLWDEPNLLAQEEYPPEVGLPGATNYAVSFTAL
                     RGRTYALERSLTLAGGSWSNAASLAEESMPWAPRTLADTNVPAVSGPVFYRVSAGAWP
                     P"
     gene            complement(6753..9125)
                     /locus_tag="PK634_07070"
     CDS             complement(6753..9125)
                     /locus_tag="PK634_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOW97534.1"
                     /translation="MAPAGGPEAGYAALAYGADAIYLGLPRFSARAEAVNFPLDDIDA
                     ITAYAHALSPRRRVFAALNTLILERELDGIIESLGALSEIGVDALIVQDLGIYRVAQR
                     HFPEFRLHASTQMAVHNRAGVERLRELGFARATLARELTLDEIRDCAAVPGIEAEVFI
                     HGTLCYAYSGLCLMSSHLLGRSGNRGRCAYLCRDRFSSPDEGKGSFLFNMKDLALPSR
                     IDALREAGVASCKIEGRMKGPLYVAATVKYYRRLLDGGLDAAGRAECEADIQSIFSRP
                     WTELHVEDRRGGEVVDPETVGHRGTPAGTVEGSRRDKGGKSWLRIAATRPIERHDGLQ
                     IDLPGQDRLFGFPVDDLRVKGRSAFESPAGAIEVGLPADCPPVPAGVTVYCASSQAVK
                     RKYRFEMPRPGVFRARRPVRVELDLKPDLLHARADAGASIVAEAGLPGQFEPAKDPAK
                     TELAARQAFEKLGDTRFELASMELRNPAGLFVPVSRLNALRRELMDALDTKRQESLSR
                     RVEAVRLAMAERAGGPRVVAAEAATWSLKIDNPASVAALDGGDWLGVEEVVVPALSAF
                     EGLVEKIGRERLRVGLPLIVRAWESAELRERIEALRAAGWEKWEATGLGSWARGPDLS
                     ADWPVYVMNTAAARQVLEMGAGRFLLSPEDGLENMRSLLARFGARAGVIVFQDTPLYI
                     AENCIQVAREGRCPGPAACEFRQIELRSSHDDRLLAVNRKCRMIVVNRSPFCLADRLP
                     ELQAAGARHFRADFSWRPYSPEEVRDLWRQLRAGRAPRGSHRGNFERGLV"
     gene            complement(9200..10495)
                     /gene="purB"
                     /locus_tag="PK634_07075"
     CDS             complement(9200..10495)
                     /gene="purB"
                     /locus_tag="PK634_07075"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOW97535.1"
                     /translation="MIPRYTRPEMGAIWTDENRFNTWLEIELLACEAMHELGEVPAAD
                     LKRIRARAKFSPRRIDEIERKVNHDVIAFLTNVAEHVGPSSRFIHKGLTSSDVLDTAL
                     SVQLVRAADRLIAGVKAVRRAVAAKARRYKYTPMIGRTHGIHAEPITFGLKMALMYDE
                     FGRALRRLESAREIVRVGKISGAVGTHAHLDPFVEKYVCRKLGLKAAALSTQVLQRDR
                     HAEYVAALALVGTSVERWAQEFRHLQRTEVREVEEFFGRGQKGSSAMPHKRNPIVAER
                     LCGLARLLRGYAVAAMENVALWHERDISHSGAERVLLPDATIALDYMLAKIEGLVRGL
                     QVYPKQMARNLALTGGLIHSQQLLLALAEKGLSREDAYGLVQFHAMESWRTGRPLKEL
                     VLGDPDVLAVLPAKEAAAVFDLAPHFRRVDRAFRLVGLA"
     gene            complement(10492..11157)
                     /locus_tag="PK634_07080"
     CDS             complement(10492..11157)
                     /locus_tag="PK634_07080"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOW97536.1"
                     /translation="MRGAEAAILTVRGMKVILDAELARLYGVPTKRLNEQVRRNTEKF
                     PPDFMFQLSAEELKAIRSKNATSSLYPADNRKDVSNWSQIATGSSQHTDNQADGSAGP
                     QTDSKSQKHRGQTYRSYAFTEHGAIMAATVLNSPQAVQMTVFVVRAFVRMREQLLGQA
                     EMEARLAEIERTLLSHDAALRTLYQKIRPLLLPPPEPPRKQIGFQVKEPRIRYSAKKG
                     RPR"
     gene            complement(11234..13501)
                     /locus_tag="PK634_07085"
     CDS             complement(11234..13501)
                     /locus_tag="PK634_07085"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOW97537.1"
                     /translation="MAMARTRDTIYLGGDFKYLGPWTGYGFVSSTATGAVRAPALDIN
                     DQVNCAISDGRGGWFIGGAFTHVGGLTRQRLARIRPDGSPDPDWAADADADAECIVLS
                     QDGTRLYVGGRFTLINGQIRTNLASLTALTGAVEAWSPLFFSTNLPQYVGALALSPDD
                     RTLYVGGNFTHINGIYRPDLAALSTETAGVTAWDPPRYISYINTLSIAPDGNTVYLGG
                     VFTNMGGQARRNLAAVTLAGAATAWNPNSSMAGITVSEILPAPSESAVYVAGTFSSLG
                     GAARIGLAKVDDVNGNALAWDAGADAGVTSLERSPDSQTLYVGGGFSRIGGQSRVRMA
                     AINAVSGVATSWFIHCNGSPLAAKFSTGGNLFIGGSFTSVGGVPRQYLAAVDDASGRV
                     TAWNPGTDYMVDHVFLSRDGHTVYVAGLFTNVGGQARFRVAALDEQGQVTSWNPNPNN
                     AVMAIEASPDGRFLYVGGAFTSIGGSSCNYLAVLDANGHARGWSAGANFHVEDLAFSP
                     DGRTLYVSGAFWELGGQPRWWLGSVDTNGVATAWNPQPSFMLDDMVLSPDGRTLFISG
                     DFDNIAGTPRNHLASVDATNGAVTAWAPNANERVYDLALSADRQTLYAVGSFTNISGQ
                     ARRHLAAYALDGTLRSWNPGIDETTAAVAPAPNDQSLAVGGSFREVAGVSRRAFALFD
                     SLAPTVRRADWSTGFRAVLTNLTVGSTATLERTASPIQPSWAFVTNTVVGSNTAASLT
                     DAWPTNRAAIYRVRL"
     gene            complement(13688..16054)
                     /locus_tag="PK634_07090"
     CDS             complement(13688..16054)
                     /locus_tag="PK634_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HOW97538.1"
                     /translation="MTAKAKGPLSRDELRKMDAYWRAANYLSVGQIYLLDNPLLEKPL
                     TLKDIKPRLLGHWGTTPGLNFLYAHFNRAIRARDLDMIYIIGPGHGGPATVAQAWLEG
                     TYSEVFPNITPDAEGLKKLFKQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAA
                     FDNPDLVVACIVGDGEAETGPLAASWHANKFLNPARDGAVLPILHLNGYKIANPTVLA
                     RIGGDELAKLFEGYGYKPYFVEGSDPAAMHQLMAATLDRALDEIRAIQQKARRGGKMV
                     RPRWPMIVFRTPKGWTGPKSVDGKPAEDSWRSHQVPLSEMSKKPGHVKRLEKWLKSYR
                     PRELFDKAGRLVPELAELPPKGARRMSANPHANGGLLLRDLRMPDFRDYAVAVKKPGT
                     AEAEATRVMGQFLRDVMKGNPCNFRVFGPDETASNRLGAVFEATDKAWMADVLATDEH
                     LAPGGRVMEILSEHTCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWLKTTRD
                     IPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKAEIIRVYLPPDANTLLS
                     VTDHCLRSRHYVNVIVAGKQPALQYLDIDAAIKHCTKGIGIWNWASNDESVEPDVVMA
                     CAGDIPTLETLAAVDILRKNFPDLKVRVVNIVDLMTLQPDTEHPHGLSDKDFDSLFTA
                     DKPVIFAFHGYPWLIHRLTYRRTNHENFHVRGYKEEGTTTTPFDMVVMNELDRFNLVD
                     DVIDRVPRLRNTAAHVKQWLHDKLIEHKQYIRRRGEDLPEIRDWKWPY"
     gene            16157..16738
                     /locus_tag="PK634_07095"
     CDS             16157..16738
                     /locus_tag="PK634_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97539.1"
                     /translation="MKRFAAISAVMMLAAAGWAEPPVSKPLAQPLSISIIYPIQLAAQ
                     DEAVHGLRLNLIYGINAEVSGLDIGLFNGVDGDVHGYQYALVNYVGGDLHGAQTAFAL
                     NLVRGNGSGEQFALVNFLDQDLTGWQVGVFNRVGSVQGCQVGLINLADDMTGLQLGGI
                     NWTTELDGLQIGVLNFAVGKESWQFLPIVNANW"
     gene            complement(16741..17238)
                     /locus_tag="PK634_07100"
     CDS             complement(16741..17238)
                     /locus_tag="PK634_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOW97540.1"
                     /translation="MQELRIEDFQENPFRLIGQQWMLITAGTREKFNTMTASWGGLGV
                     LWDRHVCFAFVRPSRYTFEFLEKSAGFTLCFFDESWRDKLMFCGSNSGRNVDKVKKTG
                     LSPVTTKGGNVWFEQARLMIECRKIAFTDLDPKHFLDPTIRQFYEGPDYHRIYIGEIE
                     RILSR"
     gene            complement(17337..17732)
                     /locus_tag="PK634_07105"
     CDS             complement(17337..17732)
                     /locus_tag="PK634_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97541.1"
                     /translation="MKRMAILMACAMAVFAAGCEDDEDSGGGSGVTGTWSGSGFYDAG
                     VALNDLTMVLTQDGDAVSGSYDITRPNRHMVGSVSGSNSGGTLDLTLTPNGTVNGTVD
                     GDTMTIEWWESGFDGRVEGGEAHLTLTRQ"
     gene            complement(17797..18375)
                     /locus_tag="PK634_07110"
     CDS             complement(17797..18375)
                     /locus_tag="PK634_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97542.1"
                     /translation="MKKSIVVLLLAVSGAWGSGTNFIVSNVPNALTIDAGIKQPLRLV
                     RGITYHFDVFVSSFYSFLLTTSSVGGSSAGMVTNGVVGFQPLFDGDLRFTPDSNTPDR
                     IYYQSPIEVNLGAPIDVFDPPELATWTATGGLHAAARNVFGPTAYVDRAASLAAQDWT
                     PVATNASGQTNLFLVLPAADPAAVYRLRMELP"
     gene            complement(18452..19432)
                     /locus_tag="PK634_07115"
     CDS             complement(18452..19432)
                     /locus_tag="PK634_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HOW97543.1"
                     /translation="MPKKEQFESAGWVTYRPQIKVVDCTVRDGGLMNSHRFTDDFVRR
                     VYQTCVAAGVDYMEFGYRASKKIFPPKDFGPWKFSGEEDIRRIAGENKTGLKICVMAD
                     AERTDYREDILPKKDSVVDCIRVACYAHQIPTAIDMVLDADEKGYETTVNLMAVSTVP
                     VFELDKALEVIAQSPAAAVYVVDSYGSLYGEQIADLVGRFAVAMKGKKEIGIHTHNNQ
                     QLAFANTIEAIIKGATRLDATMMGLGRGAGNCPMELLLSFLKNPKYLERAVLQCVQDT
                     ILPLRKELDWGYSIPYAITGVLNEHPRDAIKLRESDHPDDYVAFYDEMNE"
     gene            complement(19550..21652)
                     /locus_tag="PK634_07120"
     CDS             complement(19550..21652)
                     /locus_tag="PK634_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOW97544.1"
                     /translation="MADAVAWGGAGDLAVDDPGTVRTDVASSEQNYLHVLPGDTDADT
                     SLQAPNDVYAAAGAAWALGAATPGAVNARQASGAIRVQPANPDRDADGIPNEQDNCPD
                     TFNPVQADIDRDLLGDECDPDKDGDGALNAADNCPCDRNPDQADLDHDGLGNACDNDR
                     DGDGVSNDEDNCPDTANPDQADADHDGIGDACDSDLDGDGVANISDNCPNTPNSNQAD
                     ADRDGVGDACDNIIDSDRDGIADASDNCPNTPNPDQADADHDGIGDACDNLVDTDRDG
                     VADASDNCRDKANPGQEDLDHDGIGDACDSDLDGDGVANISDNCPNTPNSNQADADRD
                     GVGDACDNIIDSDRDGIADASDNCPNTPNPDQADADHDGIGDACDNLVDTDRDGVADA
                     SDNCRDKANPGQEDLDHDGIGDACDSDLDGDGVANQSDNCPVNANPDQADLDGNGRGD
                     ACDVLALGHVSRSFIQGACDAEDRLVIECDTFPIGAAQTVKVEYSTDRARWQSVEMVR
                     SGRPGAYEGWRREFAPFSRNEGISYRLRVTDLQGAVQVHDNVGRYFTTTTFSLTTHAP
                     EFAPMLSLLRNKYFVLINGTAAAQTVPLSMAGSVKPIEVRVRPVQYGDGSVYQASAKC
                     LTAEMLVSTNAQFRISTTISGVFHPGTPAAPDYFSFDLGMVKRPIIVYMKFKASNNLG
                     VTFLGIKPYVYTLNITKL"
BASE COUNT         3284 a         7472 c         7283 g         3619 t
ORIGIN      
        1 gccatgccgc gatcatcgtc gcgtgcatcg ccagggcgtt ggactccgcc gcgcggccga
       61 gccggtgaaa atcgcgctcg cgaatcgccg cgcgaacttc cggcaggtcg gcggccacct
      121 tcgcgggcca ggcgcggtag agcggcgatg tctcgaccgt gcgcttcatc cccgcccgcg
      181 agccgaccgc cttctcctcg cccgagatca ccacgagccc catccgcagg tccggccagg
      241 tctctgccag cggaaccgcg tagctgtcca tcccgtccgc cgcctcgccc gcccgccatt
      301 ccacgaaacc ctcgtacacc gaccggcacg cgctgccgct gccgagccgc gccaggatcg
      361 aaagctcccg cgtttccagc cgccacccga acagctgatc cagcgcgcgg accagcgccg
      421 cgaatccgga cgccgaggac gcgaagcccg cggccgtcgg gatcgtgttg cgggcgtcca
      481 cccggaagcc cgcccccgcc ggcctgaaga gatccaggta cgccgagacc cgccgggcaa
      541 acgaggaatc cggcggcaat tcctgtccgt tcaagaccac cacatccgcc ggaccggcag
      601 gttgaatctc cacctccgag cccagccggc cgagcgacac ggacaggctc gaggtcacgg
      661 gcaggttcaa ttcctcgtcc cgcttgcccc agtatttgac cagcgcgatg ttcgccggcg
      721 cgaaggcgca acccgccgcg ccgggcttca tgccccggcc cgcgaggatg cggcccacga
      781 tgtcgcgctt ggtcgccata ttttccgaac ccccgtccat tactgccggg tgagcgtccc
      841 gctgccgctg gtcccgtccc cgctggtcca tgtgtagtcc atgcgatccc ccgacacatg
      901 accggacaag tgcgtcccga cgtcatccat aatggtcagc tcgacgttgc gcccgtcgac
      961 cgagccctgc acggcggcat tggccgtcca ggtgtcgtgg tcggtgccgg tcacgttcgt
     1021 cccgttttgc acaaggatca gggtcccggt ccccgtctcg cccgattcct tgacgatctc
     1081 gcccgtccag gttcccgtca catcgacggg cgaatcgtcg ccgccgccgt cgtcctcgca
     1141 tccgaccagg acaaggccgg cgcccatcca gaggctcatc agcatcgcca ggattttatt
     1201 ccgcatcgcg tccccccgtt ttgttgcgcg cgagcccgcc tattccacct tcacgccttc
     1261 cgagctgatc tccgcctcga tccgcggccc cggccagtcg gtccggtcgg atcgccccag
     1321 cccgatgacg caatccccca gcccggaccc ggaaatcttg gagccgagga tgccgcgctc
     1381 gccgcgcagc gcgtagacca gttccgccat cctcgcgttg ctgacgccga tcgcctccat
     1441 cagcccctgc tggatgtcga gcagttcccc gaatctcttc agcgcgcccg cgcgcagcgc
     1501 ctggaagccc gccgcgcagc ccctgtccat gaggtcgaag atatcgccgt aaaaagccgg
     1561 ctgcgctttc atcgcgcgct gaacgcgccg caccacctcg ggggtcggcg tcttgccgcc
     1621 ggagtagagc acgacgagcg ggatcgtccg cttcagccgc tggacctggg ccggggcggc
     1681 gcggtagagc agaatgccgc cgaacacgct ggccgcgaca tcggcgcccg accccaatcc
     1741 ctggacttcc cgtatcacct cccggctcgc gacgaacagg tcccacggcg gcggccgccg
     1801 cgaacgcgtc cagcgctcca gcgcggcgca cgtggcgacc gtgacggcgg ccgaggagcc
     1861 cagccccacg gtggacggga agccggactc gatgttcagg tcgaagccgg acggcaagcg
     1921 ggcttcgtac cggcgcagca tggccatcac gaaccgaaac ggggccggcg agcggagccg
     1981 ccccagcggc atctccacct cgcccagcgc ggaggcgatg cggacctgcc cgtcgccccg
     2041 cggctccagc cgcacgcgga tgcgccggtt gacggcggcg acgagggcgc ggcggccgtg
     2101 gaccacggcg tgctcgccca tgagcatcag gctgcccggc gccgaggcct ggaaggaagc
     2161 ggtcaccagg cacggttgta gcagcatcgc ccgcgccgcg caatccgccg gctggttttt
     2221 tcgcccgccg ggtgtatgct tccgtagcga acggggggac ttcacggaat gaactggctg
     2281 accgaactgc acccggtgct ccaggccctg ctggcgacgc tctttacctg gggcgtgacc
     2341 gcgctgggct cggccggcgt cttctttttc aagagcgtga accgccgcgt gctggacgcc
     2401 atgctcggct tcgccgcggg cgtgatgatc gccgcgagtt tctggtcgct gctcgcgccc
     2461 tccatcgagc tggccgcgga gatgggcatg gtgaagtggt ggcccccgct ggccgggttc
     2521 ctggccggcg cgctgtttct gtacggcatc gaccgcatcc tgccgcacct gcacctgaac
     2581 ctgtcgctcg accaggccga gggcatccac acgcactgga agcgcagcat cctgctgatc
     2641 ctggccatca cgctccacaa tatcccggag gggctggccg tgggcgtggc cttcggcgcc
     2701 gtggccgccg ggatcgaatc cacctcgctg ggcggcgcga tcgccctggc ggtgggcatc
     2761 ggcctgcaga acttccccga gggcatggcc gtctccctgc ccctccgccg cgaggggatc
     2821 tcccgctgga aatgcttctg gtacggccag gcctccggcc tcgttgagcc cgtcgccggc
     2881 gtcatcggcg cggccgccgt gctgatgatg cgcccgctgc tcccctacgc gctcgccttc
     2941 gcggccggcg ccatgatctt cgtggtggtc gaggagctga tccccgagtc gcagagctcc
     3001 gagaagaccg acgtcgccac gctcggcgcg atcgccgggt tcgccgtcat gatgctcctg
     3061 gacgtggcct tgggatagcc tgcccgcgcc gcctactcca ggtcccgctc aaccagctcg
     3121 tcctcgtcca ggccgaggta gtggcccaat tcgtgaaggt aggtggtccg cacctcctcc
     3181 tgaaagatgt cctcgtcgca gtccgagtag tcggcaagat tttcgatgaa cagcaggatc
     3241 tgcgcgggca ggtcgccgcc cggcgcctcc gtcatcggaa gggcctcgcc gacaaacagg
     3301 cccagcagtt ccgggtccac gccgtcgcgc tgcatggccc gcgtgggccg gggcacgcag
     3361 gtgaccggga tctccgccgc gcggtcgcgc agcgagggcg gcaggcgccc gcgcgtgcgc
     3421 tcgacctcct gctcggcgat acggatccag cgctggaggg aggaggtcat gtggccgatc
     3481 gctgcctgac ttgtctcaag atgtcccagt acgcgccccg cgcccccgcg gcaatggcgt
     3541 ccacgagcgg cgcgttgtcg ttgtagttgt agcttttctg ccccgactcc tcgtccggcg
     3601 gcggctcgaa gaaccgcggg tcgcccgcga ggatgtcgcg aacgaccgcc gcgggacgcc
     3661 ggccgagggc cgcggcgatg tagtaggtcg gccagagcag gttgaccgga ccctcgtagc
     3721 ggcggcgaat cgccgggttg gtctccaggg ggccttccgc cgcgatccgg cgggtcagcg
     3781 gaagcccggg atacaaccgc acgcccagcg cggccccgac gcagtcgggc ttgagaatcc
     3841 gcagaaagtc caccgcttgc cgcacggtcg tgggcgtctc ccccgggccg ccaagcatga
     3901 ggtcgaccat ggtcgtgatc cctgccgcgc gcgccgcccg caccgccgcg gccaggtcct
     3961 cgactcggtg ccgaacgcca tacgcgcgga gcatcgtcgg cgtggccgcg ggcccggtga
     4021 agttgatccc gacgcacccc gcctgccgca tcgcccgcgc gaggccggcg tcgaacggcg
     4081 tgacggacag gtaggcatac caccgcaccc gctcgcccag cctgcgccgg atccattcct
     4141 cgcagaccgc ccgcgcgtgg tcgacgggca cgttgaattc gccgtcgcac aggtggaaca
     4201 cgtcgacgcc ctgccgcagg agggcctccg cctcgtccgc cgtgtcggca ggcgggcggc
     4261 accgggcgcg cgcgcccttg gcgatcgggt cggcgcagaa atcgcaggcc agcgggcagc
     4321 cgcgcttggt ctccaggccg atctgccccc cgagccggaa ataggcggca ttctccgcga
     4381 catcccgatt cgtcgggacg ttcagggggc cgtcgccgtc ccaggccggc gggttcgcgc
     4441 cgcgcggcgt gaccagcccg ggcacgcgat ccgggctgtc gccggcgctc accgcccggt
     4501 acaacgccgg cagggccgct tcgccatccc cgcggatgcc gtagtccgcc cccgtgaaat
     4561 cgaggatttc gctggcgaaa atggaaaagc ccgcgcctcc cagcacgatc ggggctttcg
     4621 taaggcggcg cagcgcgcgc acccggtcgc gcaacggctc gacgaacgac gcggcgctgg
     4681 gccagaacga atcgtccgcg ttgcgcagcg tcagcccgac gagccgcggc gagcgctcct
     4741 tgaagaacgc cttcgcggcg gccgatggat cgtcggccag acccaggtcg agccacgcga
     4801 cctcgatccc ggccgcccgg gcggcacccc ccgcgtagtc cagcccgacc ggcgcgatgg
     4861 ccggccgcat gcggttggta ttgatcagcg cgagcacggg atcaggatag cgcttttcgc
     4921 gcccgccggg aagcggcgat tcccggcctg gccgcaactg ggcatttgcg tttcgcgggg
     4981 catggggtta gcatccctcc gcttttcaca aagaggaatt tccatgcaac gacgtatcgc
     5041 gcttcttgtg ctgtgtgtct ccgcggcccg cctccaggcc gccagccccg cgcgggaagc
     5101 cgtgtccttc cggcacacct gggacgtcgg gctcggccgc agcgtgttcg tggtcggcag
     5161 ccacgcggac ctcggcgact ggagcccgga gtccgccgtg aagctgtact ggacctccgg
     5221 caatgtctgg accgggcggg tcgccgtgca ggcgggcgcg gcgctggagt acaagttcat
     5281 cgcgcggacg aattcggcgg accagtattg caacggcgac aacgtcgagt ggatggccgg
     5341 aaccaactgg cccgcgcagg tcgccgcgca gcccgacgcg ccgtacgccg gcaagaccat
     5401 cctctaccac agcggctgga ccaacgccgg gctcgtgtac cgcgtcggga ccaactggtt
     5461 tggcgcggcc atgagccggg tcggcgcggg acggaacggc ggcgagtacc tctaccaggc
     5521 ctcgggattc ggggcggccg gcgagccgat cgagttcgtt ccgtacggct attccggcgg
     5581 cgtccagtac tgggaccacg cgccttacgg cggctacggc gacagcaact actacaccac
     5641 cctggacgtc ttcttcctgc aggacgggca tgtcttcaat taccggccgc ccaccaacgt
     5701 gtccgccagc cggatcatcg gcaccaacgt cacatcgagc tgggcgccga cgatcccctc
     5761 gcgcggcgtc cgcatctacc tgccgcgcgg gtatgaccag aacacgtgga agcgctaccc
     5821 cgtgctctac atgcacgacg gcacgaacgt attccagccg ggcgggacgc tcggctgctg
     5881 gaacgccgac ctcctcgccg accgcgaaat cagccagggc cgcatgcggg agagcatcat
     5941 cgcggccgtg gacaccacgc ccaagcgcga ctcggagtat tgtccggccg gcgaccagta
     6001 cgggcccgaa ccgggcacgg gagaccagta cgccaatttc ctcgttcaca acgtgcggcc
     6061 gatggtggac acgcactacc gcacgctgaa cgatttcgac aacacgctca ccgcgggctc
     6121 gtccatgggc ggcctgatca gcgcctacct cggcctcgcc accaacgtct tcgggaaaat
     6181 cggggccttc tcccccgcgt tcctcgtcgc gccgaatttc atcgcctgga tggacgcgca
     6241 ggacacccgg ggcgtgcgca tgtacatgga cgacggcacg gtggacctcg atgtggacct
     6301 gtggcccgac acgtgggcgg cgtatgacct gctgatgcag gacgggtacg tccccaacga
     6361 cgacctgctc atggtcgtcg gctgcggcca gacccacaac gaggccgcgt gggcctcgcg
     6421 cctgccgcgg gtctaccagt acctgttgag cctctgggac gagccgaacc tgctggccca
     6481 ggaggaatac ccgccggaag tcgggctgcc gggcgcgacg aactacgccg tctccttcac
     6541 cgccctgcgc ggccggacct acgcattgga acgctcgctg acgctggccg gcggctcctg
     6601 gagcaacgcc gcctcgctcg cggaggaatc catgccctgg gcgccgcgga cgctggcgga
     6661 tacgaatgtg cccgcggtca gcggccccgt attctaccgc gtgtcggccg gcgcctggcc
     6721 gccgtaaaaa agccacctcg ttaccgaggc ggctacacga gcccccgctc gaaattcccg
     6781 cgatggctgc cgcgcggcgc gcggccggcc cggagttgcc gccaaaggtc gcggacttct
     6841 tccggtgaat aaggccgcca cgagaagtcc gcccggaaat gccgcgcgcc cgcggcctgc
     6901 agctccggca gccggtcggc caggcagaac ggcgaacggt tgaccacgat catccggcac
     6961 ttccggttca cggccagcaa ccggtcgtcg tgggaggacc gcaactcgat ctgccggaat
     7021 tcgcaggcgg ccgggccggg gcagcgccct tcccgcgcga cctggatgca gttctcggcg
     7081 atgtagagcg gcgtgtcctg gaacacgatc accccggcgc gcgcgccgaa acgggccagc
     7141 agcgaccgca tgttctccag cccgtcctcc ggcgagagca ggaaccggcc cgcgcccatc
     7201 tccagcacct gccgggcggc ggcggtgttc atgacgtaca ccggccagtc cgcggaaaga
     7261 tcgggcccgc gcgcccatga tcccagaccg gtcgcttccc acttctccca gcccgccgcg
     7321 cggagggctt caatccgctc ccgtaattcc gccgactccc aggcgcggac gatcagcggc
     7381 aggccgacgc gcaggcgctc gcggccgatt ttctccacca gcccttcaaa cgcggaaagg
     7441 gccggcacga cgacctcttc cacgcccagc cagtcgccgc catccaacgc ggcgacgctg
     7501 gccgggttgt cgattttgag gctccacgtc gccgcctcgg ccgcgacgac gcgcggccct
     7561 ccggcccgct ccgccatcgc caagcgtaca gcttccacgc gccgggagag cgattcctgc
     7621 cgcttcgtgt cgagcgcatc catcagttcc cggcgcagcg cgttgagtcg ggagaccggg
     7681 acgaagaggc ccgccggatt ccgcaattcc atggaggcca gctcgaatcg ggtgtcaccg
     7741 agcttctcga acgcctgcct cgccgcaagc tccgttttcg cggggtcctt ggccggttcg
     7801 aactggcccg gcaggccggc ttccgccacg atgctggcgc cggcatcggc gcgggcatgc
     7861 aacaagtcgg gcttcaggtc cagttccacg cggaccggcc gccgcgcgcg gaataccccg
     7921 gggcgcggca tctcgaagcg gtatttcctt ttcacggcct gcgaggaggc gcagtacacc
     7981 gtcacgcccg cgggaaccgg cggacagtcc gcgggcaggc cgacttcgat cgcgccggcg
     8041 ggcgattcga atgcggaacg cccttttacc cgcaggtcgt ccaccgggaa cccgaacagc
     8101 cggtcctggc cgggcaggtc tatctgcagg ccgtcatgcc gctcgatcgg gcgcgtcgcg
     8161 gcgatccgca gccacgactt cccccccttg tcgcgccgcg agccttccac cgtgcccgcc
     8221 ggcgtcccac ggtggccgac cgtttccggg tccacgacct cgcccccgcg ccggtcttcg
     8281 acatgcagct cggtccacgg ccggctgaag atcgactgga tatccgcctc gcattccgcg
     8341 cggcccgccg catcgagtcc gccgtccagc aggcggcggt aatacttcac cgtcgcggcg
     8401 acgtagagcg ggcctttcat gcggccttcg attttgcagg acgccacgcc ggcctcgcgc
     8461 agggcgtcga tccggctcgg cagcgcgaga tccttcatgt tgaacaggaa cgagcccttg
     8521 ccctcgtccg gcgaggagaa ccggtccctg cagaggtacg cgcaccggcc gcggtttccg
     8581 ctgcggccca gcaggtgcga cgacatcagg cacaagccgc tgtacgcgta gcagagcgtg
     8641 ccgtgaatga agacctcggc ctcgatcccc ggtacagcgg cgcagtcgcg gatttcgtcc
     8701 agcgtcagtt cgcgggcgag cgtcgcgcgc gcgaagccca gctcgcgcag ccgctccacg
     8761 cccgcgcggt tatggacggc catctgcgtg ctggcatgga gccggaattc gggaaaatgt
     8821 ctttgggcca cccggtaaat gccgaggtcc tgcacgatca gcgcgtccac gccgatctcc
     8881 gagagcgcgc ccagcgactc gatgatcccg tccagctccc gctccaggat cagcgtgttc
     8941 agcgcggcga acacgcggcg gcgcggcgac agggcgtgcg cgtaggcggt gatggcgtcg
     9001 atgtcgtcca gcgggaaatt gaccgcctcc gcgcgcgcgg aaaaccgcgg caggcccagg
     9061 tagatcgcgt ccgcgccgta cgccagcgcg gcataacccg cctccggccc gcccgccggc
     9121 gccatgagtt ctattccgtg tcggcgtcgc ggagctccgc cccccgaatc cttgcgcgct
     9181 gcattcatga aggggcgagt tacgccagcc cgaccagccg gaacgcccgg tccacgcggc
     9241 ggaagtgcgg ggcgaggtcg aagacggccg cggcttcttt cgcgggcagg acggccagga
     9301 catcgggatc gccgaggacc agttccttca gcggccgccc ggtgcgccag ctttccatgg
     9361 cgtggaactg gaccagcccg taggcgtcct cgcgggagag ccctttctcc gccagggcga
     9421 ggagcaactg ctgggaatgg atcagcccgc cggtcagcgc caggttgcgc gccatctgtt
     9481 tcgggtagac ctgcaggccg cgcacgagcc cctcgatctt cgcgagcatg tagtccagcg
     9541 cgatcgtcgc gtcgggcagg agcacgcgct ccgcgccgga gtgcgagatg tcgcgctcgt
     9601 gccagagcgc gacgttctcc atcgcggcca ccgcgtagcc gcgcaggagc cgggcgaggc
     9661 cgcaaagccg ttcggcgacg atcgggttgc gcttgtgcgg catggcggac gagcccttct
     9721 gcccgcggcc gaagaactcc tcgacctcgc gaacctcggt ccgctggagg tgccggaact
     9781 cctgcgccca gcgctcgacc gacgtcccga ccagcgccag cgcggcgacg tactccgcgt
     9841 gccggtcgcg ctggagcacc tgggtcgaga gcgcggccgc cttcaggccc agctttcggc
     9901 aaacgtattt ctccacgaac gggtcgagat gcgcgtgcgt gccgaccgcg ccggagatct
     9961 tgccgacccg gacgatttcc cgggccgatt ccaggcggcg gagggcgcgg ccgaattcgt
    10021 cgtacatcag ggccatcttg aggccgaacg tgatcggctc ggcgtggatc ccgtgcgtgc
    10081 ggccgatcat cggcgtatac ttatagcgtc gcgccttcgc ggcgacggcg cggcgcaccg
    10141 ccttgacgcc ggcgatcagc cggtccgccg cccggaccag ctggacggac agcgcggtgt
    10201 cgagcacgtc ggagctggtc aggcccttgt ggatgaagcg cgaggacggg ccgacgtgct
    10261 ccgcgacgtt cgtgaggaag gcgatgacgt cgtggttgac cttccgctcg atctcgtcga
    10321 tgcggcgcgg gctgaacttc gcgcgggcgc ggatgcgctt gagatcggcg gcgggcacct
    10381 cgccgagctc gtgcatcgcc tcgcaggcca gcaactcgat ctcgagccag gtgttgaacc
    10441 ggttctcgtc cgtccagatc gcgcccatct cgggccgggt gtaacggggg atcatcgcgg
    10501 gcgtcctttc ttcgcggaat accgaatgcg cggctccttg acctggaagc cgatctgttt
    10561 tctcggcggc tccggcggcg gcaggagcag cgggcggatt ttctgataaa gagttcttaa
    10621 cgccgcatcg tgggagagca acgttcgttc aatttcggcc aaccgcgctt ccatctccgc
    10681 ctgccccagc agttgctcac gcatgcggac aaacgcccgc acgacgaaca cggtcatctg
    10741 caccgcctgt ggactgttca gaaccgttgc cgccatgatc gcgccgtgtt cggtgaaggc
    10801 gtaagatcgg taggtttgcc cccggtgttt ttgagacttg ctgtccgttt gcggcccagc
    10861 cgaaccatct gcttgattat cagtatgttg cgatgaaccg gtcgcaattt gcgaccagtt
    10921 cgacacatct ttcctattat cagcaggata aagagaactg gtcgcatttt tcgacctgat
    10981 ggcctttagc tcctctgcag aaagctgaaa catgaaatcg ggcgggaatt tctcggtatt
    11041 ccgccgcacc tgctcgttga gccgcttggt cggcacgccg tacaggcggg ccagttcggc
    11101 atcgaggata actttcatcc cccgcaccgt caggatcgcg gcttcggctc cacgcatttt
    11161 ttcaggtttc acggtcattc ggatcggccc cggccctctc ataccgtttt cgggccgggg
    11221 gaacaacggc attctacagc cgcacgcggt agatcgccgc gcggttggtg ggccaggcgt
    11281 ccgtgaggct cgcggccgtg ttgctgccga cgacggtgtt tgtgacgaag gcccaggacg
    11341 gctggatcgg cgacgcggtc cgctcgagcg tggccgtgct cccgacggtc aggttggtca
    11401 ggacggcgcg gaagcccgtg gaccagtccg cgcgccggac cgtcggcgcg agggaatcga
    11461 agagagcgaa cgcccgccgg gacacgcccg ccacctccct gaacgagccg ccgaccgcca
    11521 gcgactggtc gttgggcgcg ggcgcgacgg ccgccgtcgt ctcgtcaatc ccgggattcc
    11581 aactccgcag cgtcccgtcc agcgcatagg cggcaaggtg gcgtcgggcc tgcccgctga
    11641 tgttggtgaa ggatccgacg gcgtaaagcg tttgccggtc ggcggaaagg gccaggtcat
    11701 aaacccgctc gttggcgttg ggggcccatg ccgtcaccgc gccgttggtc gcgtccacgg
    11761 aggcgaggtg gttccggggc gtgccggcga tgttgtcgaa atcgccactg atgaagagcg
    11821 tccggccatc gggcgagagg accatgtcgt cgagcatgaa gctgggctgc ggattccacg
    11881 ccgtggcaac gccgttggta tccaccgacc cgagccacca gcgcggctgc ccgccgagtt
    11941 cccagaacgc gcccgagacg tacagcgtcc ggccgtccgg cgagaaggcg agatcctcga
    12001 catggaagtt agcccctgcg ctccagccgc gcgcgtggcc gttggcgtcg aggacggcca
    12061 gatagttgca gctgctgccg ccgatgctgg tgaacgcgcc gcccacgtac aggaaccggc
    12121 cgtcgggcga ggcctcgatg gccatgacgg cgttgttcgg attcgggttc cacgaagtca
    12181 cctgcccctg ctcgtccagc gccgccacgc ggaagcgggc ctggcccccg acgttggtga
    12241 acaggccggc gacgtagacc gtgtgcccgt cgcgcgacag gaagacatgg tctaccatgt
    12301 agtcggtccc ggggttccaa gcagtcactc gcccgctggc gtcgtccacg gcggccaggt
    12361 attggcgggg cactccgccg acgctggtga agctgcctcc gatgaacaag ttcccgccgg
    12421 tggagaactt ggcggccaac ggactgccgt tgcaatgaat gaaccaggac gtcgccacgc
    12481 cgctgaccgc attgatggcg gccatgcgga ctctcgattg gcctccgatc cgcgagaacc
    12541 cgccgcccac gtagagggtt tgactgtcgg gcgagcgttc gagactggtc accccggcgt
    12601 ccgcgcccgc atcccaggcc agggcattgc cgttgacgtc atccaccttg gcgagtccga
    12661 tccgcgcggc cccgcccagg gagctaaacg tgccggcgac gtacacggcg gattccgacg
    12721 gggccggcag gatttcggaa acggtgatac cggccatgct cgagttcgga ttccacgccg
    12781 tggcggcgcc cgcgagcgtc acggcggcca ggttgcgccg ggcctgcccg cccatgttgg
    12841 tgaaaacgcc acccaggtag accgtattcc catcgggggc gatggaaagc gtgttgatat
    12901 acgagatgta tcgcggcggg tcccaagcgg tcactccggc ggtctcggtg gacagcgcgg
    12961 ccaggtcggg gcggtaaatg ccgttgatgt gagtaaaatt gccgccgacg tacagcgtcc
    13021 tgtcgtccgg agaaagcgcc agggcgccca cgtattgcgg caggttggtc gagaaaaata
    13081 aggggctcca ggcctcgacc gcgccggtca gggcggtgag cgacgcaagg ttggtgcgga
    13141 tctgaccgtt gatgagggtg aagcggccgc cgacgtagag gcgggtgccg tcctggctca
    13201 acacaatgca ttccgcatcg gcgtcggcgt cggccgccca gtccggatcc ggcgagccgt
    13261 cgggccggat gcgggccaga cgctgccggg tgagtccgcc gacgtgggtg aacgcgccgc
    13321 caatgaacca gccgccgcgt ccgtctgaaa tggcgcaatt cacctggtca ttgatgtcca
    13381 acgcgggggc ccggacggcg cccgtcgccg tgctggaaac gaaaccgtat cctgtccacg
    13441 ggccgaggta cttgaaatca ccgccgaggt aaatcgtgtc gcgcgtccgg gccatggcca
    13501 tgacgcggtg gtccgttatc caagcggacg cgcgcgggac ggattgcggc tcatcggcga
    13561 aagcggccgt gcagagcatt ccaagaaccg cgatccaccg cctggtccct tgccgggcca
    13621 tcctcaccct ccgcggacaa gaatatacga ttcccgtccg ggagaaaagc agagggacta
    13681 gccgcgctca atacggccac ttccagtcgc ggatctcggg caaatcttcg ccgcgccggc
    13741 ggatgtattg cttgtgctcg atgagcttgt cgtggagcca ctgcttcacg tgcgcggcgg
    13801 tgttgcgcag gcgcggcacg cggtcgatga cgtcgtccac gaggttgaac cggtcgagct
    13861 cgttcatcac gaccatgtcg aacggcgtgg tggtcgtgcc ctcctccttg tagccgcgga
    13921 cgtggaagtt ctcgtggttg gtccggcggt aggtgagccg gtggatcagc cacgggtagc
    13981 cgtggaaggc gaagatcacg ggcttgtcgg cggtgaagag gctgtcgaag tccttgtccg
    14041 agagcccgtg cgggtgctcc gtgtccggct gcagggtcat caggtcgacg atattgacca
    14101 cgcggacctt caggtccggg aagttcttcc gcaggatgtc cacagccgcg agcgtctcga
    14161 gggtcgggat gtctccggcg caggccatga ccacgtccgg ttcgacgctc tcgtcgttgc
    14221 tggcccagtt ccagatgccg atgcccttgg tgcagtgctt gatcgccgcg tctatgtcga
    14281 ggtactgcag cgcaggctgc ttgccggcga cgatgacgtt gacgtagtgc cggctgcgca
    14341 ggcagtggtc ggtgacggag agcagcgtgt tcgcgtcggg cgggaggtag acgcggatga
    14401 tctcggcctt cttgttgacc acgtggtcga tgaaccccgg gtcctggtgg ctgaacccgt
    14461 tatggtcctg ccgccagacg tgcgaggtga gcaggtaatt gagcgaggcg atcggccttc
    14521 gccacgggat gtcccgcgtg gtcttgagcc acttggcgtg ctggttgaac atggaatcca
    14581 cgatgtggat gaacgcctcg taacaggaga agaagccgtg gcggccggtc agcaggtagc
    14641 cctcgagcca gccctggcac gtgtgctcgc tcaagatttc catgacgcgg ccgcccggcg
    14701 cgaggtgctc gtcggtcgcc agcacgtcgg ccatccaggc cttgtccgtt gcctcgaaca
    14761 ccgcgccgag ccggttagac gccgtctcgt cgggcccgaa cacccggaaa ttacaggggt
    14821 tgcccttcat cacgtcgcgc aggaactggc ccatgacgcg ggtggcctcg gcctcggccg
    14881 tgccgggctt tttcacggcg acggcgtaat cgcggaagtc gggcatgcgc aggtcgcgca
    14941 ggagcaggcc gccgttggcg tgcgggttgg cgctcatgcg gcgcgcgcct ttcgggggga
    15001 gttcggccag ttcggggacc agccggccgg ccttgtcgaa cagctcgcgc ggccggtagc
    15061 tcttcagcca tttctcgagc cgcttgacgt ggccgggctt cttcgacatc tcggagagcg
    15121 ggacctggtg cgagcgccag gaatcctcgg cgggcttgcc gtccacggac ttcggtccgg
    15181 tccagccctt gggggtgcgg aagacgatca tcggccagcg cggccggacc atctttccgc
    15241 cgcgccgcgc tttctgctga atggcgcgaa tctcgtcgag cgcgcggtcc agcgtcgcgg
    15301 ccatgagctg gtgcatggcc gccgggtcgg acccctccac gaagtagggc ttgtagccgt
    15361 agccctcgaa cagcttcgca agctcgtcgc cgccgatccg cgcgaggacg gtcgggttgg
    15421 cgatcttgta gccgttgagg tggaggatcg gcagcacggc cccgtcgcgg gccgggttga
    15481 ggaacttgtt cgcatgccag ctcgcagcca gcgggccggt ctccgcctcg ccgtcgccga
    15541 cgatgcaggc gacgacgagg tccgggttgt cgaacgccgc gccgtacgcg tgcgagaccg
    15601 cgtacccgag ctcgccgccc tcgtggatgg agccgggcgt ctcgggcgcg acgtggctcg
    15661 ggatgccgcc ggggaacgag aactgcttga agagcttctt caggccctcc gcgtccggcg
    15721 tgatgttcgg gaacacctcg ctgtacgtcc cctccagcca ggcctgggcg acggtcgcgg
    15781 gcccgccgtg gccggggccg atgatgtaga tcatgtccag gtcgcgcgcc cggatggcgc
    15841 ggttgaagtg cgcgtagagg aagttcaggc cgggcgtcgt gccccagtgc ccgagcaggc
    15901 gcggcttgat gtccttcagc gtcagcggct tttccagcag ggggttgtcc agcaggtaga
    15961 tctggcccac ggacaggtag ttcgcggccc gccagtaggc gtccatcttt cgcagctcgt
    16021 cgcgggacag gggtcccttg gccttcgcgg tcatgttggt tctccgggtg aaacgcggaa
    16081 cttgttgaaa ttatcgcccg gttcgcccaa acttcaaccc gtaatccgtg aagacggcca
    16141 accaggaggg aaagccatga agcggttcgc ggcgatttcg gcggtcatga tgctggcggc
    16201 ggcgggatgg gcggagcctc ccgtgtccaa gcctctcgcc cagcccctgt cgatctccat
    16261 catctatccc attcagctgg ccgcgcagga tgaggcggtc cacgggctcc ggctcaacct
    16321 gatctacgga atcaacgccg aggtcagcgg cctggacatc ggcctgttca acggcgtcga
    16381 cggggacgtg cacggctacc agtacgcgct ggtcaactac gtgggcggcg acctgcacgg
    16441 cgcgcagacc gccttcgcgc tcaacctggt ccggggcaac ggatccggcg agcagttcgc
    16501 cctggtcaat ttcctcgacc aggatctcac cggctggcag gtgggcgtgt tcaaccgcgt
    16561 cggctccgtg cagggctgcc aggtcgggct catcaacctg gccgacgaca tgacggggct
    16621 gcagttgggc ggcatcaact ggaccaccga gctcgacggc ctgcagatcg gcgtgctgaa
    16681 tttcgccgtc ggcaaggagt cctggcagtt cctgccgatc gtcaacgcga actggtaagg
    16741 ctacctcgac aggatccgct cgatctcccc gatgtagatc cggtggtaat ccgggccctc
    16801 gtagaactgg cggatcgtgg ggtcgaggaa gtgcttcggg tcgaggtcgg tgaaggcgat
    16861 cttccggcac tcgatcatca gccgcgcctg ttcgaaccag acgttcccgc ccttcgtcgt
    16921 cacgggcgag aggccggtct ttttcacttt gtccacgttc cggccggagt tggagccgca
    16981 gaacatcagc ttgtcccgcc agctctcgtc gaagaagcac agggtaaagc ccgccgactt
    17041 ttccaggaac tcgaacgtgt agcgcgaggg ccggacgaag gcgaagcaga cgtggcggtc
    17101 ccagagcacg ccgagcccgc cccaactcgc ggtcatggtg ttgaatttct cccgcgtccc
    17161 ggcggtgatc agcatccact gctggccgat cagccggaac gggttctcct ggaaatcctc
    17221 gatccgcagt tcttgcatcg ggctcatgcc ggcctcccgt gtcctgtccg ttaccgaacg
    17281 atagcgcaac ccggccgccg gcccaaccgt cccgccgcgg gcgggcgccc cggcgtttac
    17341 tgccgggtca gggtaaggtg ggcctccccg ccttccaccc ggccgtcgaa gccgctctcc
    17401 caccactcga tcgtcatcgt gtccccgtcc acggtgccgt tcacggtccc gttgggcgtg
    17461 agcgtgaggt ccagggtgcc gccgctgttc gatccggaca cgctgccgac catgtgccgg
    17521 ttcgggcggg tgatgtcgta ggagccgctc accgcgtccc cgtcctgggt gagcaccatc
    17581 gtcaagtcat tgagcgccac cccggcgtcg taaaagccgg agccgctcca cgtgccggtg
    17641 acgcccgagc cgcccccgct gtcctcgtcg tcctcgcagc cggctgcaaa caccgccatg
    17701 gcgcaggcca tcaggatcgc cattcttttc atggagccct cctgcgggca gtatgcccct
    17761 gcgctccgcg cggcgcaagc ccggccttcg cccgcgtcag ggcaactcca tgcgcaggcg
    17821 gtaaacggcc gcgggatcgg cggcgggcaa aaccaggaag aggttggtct ggccgctggc
    17881 attcgtcgcc acgggggtcc agtcctgggc cgccaggctg gcggcccggt ccacgtaggc
    17941 ggtgggcccg aagacgttgc gcgccgcggc atgcaagccg cccgtcgcgg tccaggtcgc
    18001 cagttccggc ggatcgaaga cgtcgatcgg ggccccgagg ttcacttcga tcggggactg
    18061 gtagtagatc cggtcgggcg tgttgctgtc gggcgtgaag cgcaggtcgc cgtcgaagag
    18121 cggctggaag ccgacgaccc cgttcgtcac catcccggcc gaggacccgc cgacggagga
    18181 cgtggtcagg aggaacgagt agaaggagga cacgaacacg tcgaagtggt acgtgatccc
    18241 gcgcaccagc cggaggggtt gcttgatgcc cgcgtcgatg gtcagcgcgt tcggcacgtt
    18301 gctgacgatg aaattcgtgc cggagcccca cgcccccgag accgccaaca gcaggacgac
    18361 tatcgacttt ttcatgcgcg cccccgcttg gaacggacaa tatagtccgt ccagcgccac
    18421 attcaagggg cgcgccgccg gcgcgtcgcg gctactcgtt catctcgtcg tagaacgcca
    18481 cgtagtcgtc ggggtggtcg ctctcgcgca gcttgatggc gtcgcggggg tgctcgttga
    18541 gcacgccggt gatggcgtac gggatgctgt agccccagtc caactccttc cgcagcggca
    18601 ggatggtgtc ctggacgcac tggaggacgg cgcgctcgag gtacttgggg ttcttcagga
    18661 agctcaacag cagttccatg ggacagttgc ccgccccgcg ccccaggccc atcatcgtcg
    18721 cgtccagccg cgtggcgccc ttgatgatcg cctcgatggt gttcgcgaag gcgagctgct
    18781 ggttgttgtg cgtgtggatg ccgatctcct tcttgccctt catcgccacg gcgaaccggc
    18841 cgaccaggtc ggcgatctgc tcgccgtaca gcgagccgta gctgtccacg acgtacacgg
    18901 cggcggcggg gctctgcgcg atcacctcca gcgccttgtc gagctcgaac acggggaccg
    18961 tggacacggc catcaggttg acggtcgttt cgtagccttt ctcgtcggcg tccagaacca
    19021 tgtcgatcgc cgtcgggatc tggtgcgcgt agcaggccac gcggatgcag tccaccacgc
    19081 tgtccttctt ggggaggatg tcctcgcggt agtccgtgcg ctcggcgtcg gccatgacgc
    19141 agatcttcag gccggtcttg ttctcgcccg cgatgcggcg gatgtcctcc tcgccgctga
    19201 acttccaggg gccgaaatcc ttgggcggga agatcttctt cgaggcgcgg tatccgaact
    19261 ccatgtagtc cacgcccgcg gccacgcacg tctggtagac gcggcggacg aagtcgtcgg
    19321 taaaccggtg gctgttcatc aggccgccgt cccggaccgt gcagtccacg accttgatct
    19381 gcgggcggta cgtgacccag cccgcgcttt cgaattgctc tttcttcggc atacgctgac
    19441 cctcccccgc gcccggccgg gcgcgggacc aagagtatgc cccaatcgcc ggatttctca
    19501 agctccggcg gcggcgcgcc ggcgcgcggg acgccgcggc ccggcctcgt cagagcttgg
    19561 tgatgttcag ggtatacacg tagggcttga tgcccaggaa ggttacgccc aggttgttgc
    19621 tcgccttgaa cttcatgtag acgatgatcg gcctcttcac catccccagg tcgaagctga
    19681 agtagtccgg cgccgccggc gtgccggggt ggaagacgcc ggagatcgtc gtggagatcc
    19741 tgaactgcgc gtttgttgac accagcattt cagccgtcag gcacttggcc gacgcctggt
    19801 agacgctgcc gtccccatat tgcacggggc gcactcgcac ctcgatgggc ttgacggagc
    19861 ccgccatgga cagcggcacg gtctgcgccg cggccgtccc gttgatgagc acgaagtact
    19921 tgttgcgcag gagggaaagc atcggcgcaa actcgggggc gtgcgtcgtc aagctgaacg
    19981 tcgtcgtcgt gaagtagcgg ccgacattgt cgtgcacctg cacggcgccc tgcaggtcgg
    20041 tcacccgcag ccggtagctg atcccctcgt ttctcgaaaa cggagcgaac tcgcgccgcc
    20101 agccctcgta ggcgcccggc cggccggaac ggaccatctc gaccgactgc cagcgggccc
    20161 ggtccgtgct gtattcgacc ttcaccgtct gggcggcgcc gatggggaac gtgtcgcact
    20221 cgatgaccaa acggtcctcg gcgtcgcagg cgccctgaat gaacgagcgg ctcacatggc
    20281 ccagcgcgag aacatcgcac gcatcccccc gcccgttgcc gtccaggtcc gcctggtccg
    20341 ggttggcgtt caccgggcaa ttgtcggact gattggccac gccgtcgccg tccaggtcgg
    20401 agtcgcaggc gtcgccgatc ccgtcgtggt ccaggtcttc ctgcccggga ttcgccttgt
    20461 cccggcagtt gtccgacgcg tcggccaccc catcccggtc cgtgtccacc agattgtcgc
    20521 acgcgtcccc gatgccatcg tggtccgcgt cggcctggtc cgggttcggc gtgttcgggc
    20581 agttgtcgga ggcatcggca atcccgtcgc gatcggagtc gatgatgttg tcacaggcat
    20641 cgccgacgcc gtcgcggtcg gcatcggcct ggttggagtt cggcgtgttc gggcagttgt
    20701 cggagatatt ggccacgccg tcgccgtcca ggtcggagtc gcaggcgtcg ccgatcccgt
    20761 cgtggtccag gtcttcctgc ccgggattcg ccttgtcccg gcagttgtcc gacgcgtcgg
    20821 ccaccccatc ccggtccgtg tccaccagat tgtcgcacgc gtccccgatg ccatcgtggt
    20881 ccgcgtcggc ctggtccggg ttcggcgtgt tcgggcagtt gtcggaggca tcggcaatcc
    20941 cgtcgcgatc ggagtcgatg atgttgtcac aggcatcgcc gacgccgtcg cggtcggcat
    21001 cggcctggtt ggagttcggc gtgttcgggc agttgtcgga gatattggcc acgccgtcgc
    21061 catccaggtc ggagtcgcag gcgtcgccga tcccgtcatg gtccgcgtca gcctggtcgg
    21121 gattagccgt gtcggggcag ttgtcctcgt cattcgaaac accgtccccg tcgcggtcgt
    21181 tgtcgcacgc attaccgagc ccgtcgtggt ccaggtcggc ctggtccggg ttccggtcgc
    21241 aggggcagtt gtccgcggcg ttcagcgcgc cgtccccgtc cttgtccggg tcgcactcgt
    21301 cgcccaggag atcccggtcg atgtccgcct ggacgggatt gaacgtgtcc gggcagttgt
    21361 cctgctcgtt cgggatgccg tccgcgtccc ggtccggatt ggccggctgg acgcggatgg
    21421 cgccgctcgc ctgccgggcg ttgacggcgc ccggggtagc ggcgcccagc gcccaggccg
    21481 cgcccgccgc ggcgtagaca tcgttgggcg cctggaggct cgtgtcggcg tcggtatcgc
    21541 ccgggagaac gtgaaggtaa ttttgctcgg aggaagccac gtcggtccgc accgtgcccg
    21601 gatcgtccac ggcgaggtcg ccggcgccgc cccaggcgac ggcatcggcc acggcgcc
//
LOCUS       DAOOTK010000047        21460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1688_length_21460_cov_5.046858, whole genome shotgun sequence.
ACCESSION   DAOOTK010000047 DAOOTK010000000
VERSION     DAOOTK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21460
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1688_length_21460_cov_5.046858"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3967
                     /locus_tag="PK634_07125"
     CDS             <1..3967
                     /locus_tag="PK634_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97545.1"
                     /translation="AATDLAADTDGDGFKNFLADLDPPLFNTDDNSGHPNHVAGGDRL
                     MRIAGSTTDPLEADSDGDGLPDGIEDANRNGRLDAGETDPNNSDTDGDGKLDGTEDIS
                     HDGLVDGDSNTNRLYDAGEAWLETDPLNADTDGDGLPDGWENRSCLSALDNGTNSLAT
                     AAAGDGTADFGPAGNPDLDALSNLQEYTGGTDPCYADNGQAEPGDQITIGAGKSLGTL
                     AGVDVSEQFMDWTADDCHTLDEVHGAGNLRERGDVYFWDDDYDTSRDLVAFYSRDGRD
                     LNEFYFRLDFYDLQYQAEEGHMDFYVVIDTGNTSTGEKALPDSVNCASDMGWELVVAV
                     YETGIANVLIDQDPLLNTVNSWDDLSAHGVVSRPDFIRGVSFRADYDAVEVAIDRAAL
                     IQAGWNGISELHYQVYTTRDGTQDSPRGPGDVGGTSDLADAILDDERGSDDAVLNGWI
                     SQGWKANRAKLAVLLHGNQPAVPASEIQKQIRSGAGDSATGYFRALDANEFFGLPLNL
                     RISGQLASTIEWAASDTDPQKDGPSFNARIRRMVREGQAALLAGPYAEQALPFFSSTL
                     NQASLEMGQLALNTIYGTAFPNWWDVYFYPERMMDGPTLTQLAGLGAYFGVVDQYTHL
                     YNWFGREAALKEEGFKLNEVNGVKLFAINQQAADDIFVRHDGGLSLGLRGLLSKKANE
                     WWARDQAVVMFSLWENFGNTTNAAAYDRNLDWIANHPWIQVVTLEQIAKGEVDITGDG
                     AGDYWSTVARGSGLALSPQANDYVHFSSGSDFGNWYDGSANEESLRDRKFTYEGATQT
                     VKTFGDAGTAGTILGDAWAAVAGAGTSREGLLGRMVLGQGLWETAFHNEDETDRSRWS
                     YGAYKHPDSNYDTLAGFALTAQAQARLGALYGRVAQWATALPAGATNTAEDVDLDGES
                     EYVLCNQRVFAVFEDNGGRMIAAFVRDPASGRVFQGIGNTLAYAEGESEDEGVSNLDG
                     SGNPLAKRTSALKDWWAAGPNTNGYVNSLYAAAAVAGGWRLTSADGKIQKTVTLASAT
                     ATTFEVQYQVQPSITALYVRHGLAPNLYSLALNGQKYLSGPTDAGGRVNLAQDDRVDT
                     VAVSVGYADAGHGAAYNPAAVDHGGAFAARNLRNQAQVQQVEFSGSGTFSFDIRLSAG
                     RTDTDGDGTPNESDTDDDNDGYSDTIEQTVDPLTGEAQSDPWNAGSVPPDIDGDGITD
                     ALDADRDGDGVSDDDEFLAGTDPRDGNSALVLQAIVKPAGGRARIEWPTVGGRRYTVG
                     YARNVAGQGLVWTDIPESAFQVWETDVAEGEADTESFTDDGTLTGSPAPVAYRVHAHA
                     P"
     gene            4070..5776
                     /gene="gspE"
                     /locus_tag="PK634_07130"
     CDS             4070..5776
                     /gene="gspE"
                     /locus_tag="PK634_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOW97546.1"
                     /translation="MTANDEYILEILESVGLINREQGRAAREAAQAEDKNVIETLSAS
                     GAVSKMDILKALANQFGMETITLTGLEIPQDVLDMVPGEVARRYKIVPVFKNDNMLTV
                     ALSDPLDVETLDSLRYVLKCNVEGVVAPAEEIETALNHYYAHSGTTVESMLEEITEGT
                     VAMPTGGQILAGEGEVTEADAPIIKLVSLIIMEAFRNRASDIHLEPLAKKFRVRYRID
                     GVLHEVDSPPKRLQSAVISRLKIMGNMSIAEKRLPQDGRIQINVMGRDLDLRVSSIPA
                     SHGESIVMRILDKQSLALGLPQLGFFSDDQQTFERLIGLSDGILLVTGPTGSGKTTTL
                     YACLNYINRPDRKIITVEDPVEYQMSGINQVHVRTDIGLTFAAALRSILRQAPNIIMI
                     GEIRDMETAEIAVNASLTGHLVFSTLHTNDAPSAIIRMIDIGVKPFLVASSTRAIMAQ
                     RLVRKTCEKCKEPYKATDAELRLLGPAAHQLAQAQLFRGKGCSDCNFTGYRGRLGIFE
                     IFIINDEVRHMIFERVSATELRSKARGLGMRTLREDGLRKVVAGVTTLPEVLRVTMGD
                     EG"
     gene            5776..6873
                     /locus_tag="PK634_07135"
     CDS             5776..6873
                     /locus_tag="PK634_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOW97547.1"
                     /translation="MSTSASQIGMSDLLSLVVSEGASDLHLAVGVPPVLRLHGSLHPL
                     DADVLKPEDTERLMKSITSDEHQQRVKEQGGTDFGFGFGDLARFRVSVFKQKGHIGLV
                     LRLIPSKLMSLEEIGLPAQVKDLLFRPRGLILVTGPTGSGKTTTLASMINIINEERDC
                     HIITIEDPVEYYHSHKKSVITQREIGVDVPAFKEALRRGLRQDPDVILVGEMRDLETM
                     EAAITAAETGHLVFATLHTTGAARTVDRIVDAFPTNQQEQIRTQLASSIVAVISQLLL
                     VRKDKPGRIAAFEIMIATPSIRSLIRDNKTFRITSDIQTGARWGMMTLDAHLMALYER
                     NLIGYEDLITKPQDPEAVYQKLEASGLRPRK"
     gene            6879..8591
                     /locus_tag="PK634_07140"
     CDS             6879..8591
                     /locus_tag="PK634_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOW97548.1"
                     /translation="MAKADYSDALLQALSEEQIITSQQAEEVLDEHERTGKPVREVLM
                     DMDMVSEERLLDVIAAQLGTGIVNLHDLEISQDVVRTVPGSIARMYNVVPVELGPNSV
                     TLATYELLSPEVVDELQFVLTRDVSFVVAREEDIKSYVGRYYGDESESVSDMLSALES
                     ELQDAGDILKTGARGDDIANIEAAASSTPVVRFVNLVLYQAVQDRASDIHFEPFENEF
                     KIRYRVDGALYEMAPPPKHLALPVTSRLKVVAGLNIAERRIPQDGRIQLTVAGRPIDF
                     RVSTLPTQFGESVVLRVLDRSTVSLDLENLGMPDDVFQAFLEDIDKPNGIIIVTGPTG
                     SGKTTTLYSALRRINTIESKLLTAEEPVEYDIEGLIQNPINDAIGLTFARMLRAFLRQ
                     DPDIIMVGEVRDIETAEISIQASLTGHLVFTTLHTNDAAGAITRLIDMGVEPFLIAST
                     LEAVLGQRLVRTICQNCKTPYQPEKDILDQLGLSLESVGGRPFYYGAGCTYCNQTGYK
                     GRCGIYEYLRVRDPVRALINERKPTLIIRDKAIELGMRTLREDGIRAIFDGQTTVEEV
                     LKYT"
     gene            8634..9893
                     /locus_tag="PK634_07145"
     CDS             8634..9893
                     /locus_tag="PK634_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOW97549.1"
                     /translation="MAKFSYTALDAKGRETQGEIEADNQTSALSKIREKGLFPTQVAD
                     AAAKPAKKKATKGAGGMMKMELRMPAFLSRVKTKQLMVWTRQLATLVNAGLPLLRGLR
                     VLTKQEKNPLLKRTVSEMAESIESGSTFAEALAQHPRIFNKLFVNMVKAGEVGGVLDV
                     VLLRLAEFMEKAQKIKSKVVSAMVYPVVVLIMAGSILTFLMIFIVPKFQDIFADLLEG
                     EQLPGLTRMVLSVSKTMTSRLPIVVGIVVILVVLAKILAKARFGRLVMDNLKLKMPIF
                     GTLVRKTAIARFTRTLGTLMTSGVPVLQALNIVRDTVQNEVISKAVGLIHDSVKEGEN
                     MTRPIEACGVFPPMVVSMVEVGEETGGLPEMLMKIADSYDDDVDNTVVGLTSIIEPIL
                     IIFLAIIVGTIVIALFLPLISIIGKMS"
     gene            9922..10500
                     /locus_tag="PK634_07150"
     CDS             9922..10500
                     /locus_tag="PK634_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97550.1"
                     /translation="MTGRRIRPSTAIAAGIALLLVSWIVPGFWHARRQRAQAYARAGA
                     RALQQAAQHFYSDYGVWPVDQRVSVRDIHFGDARPNAEVLNALRGVAGPGNEDHAVNP
                     HRTVYFDPLAAGPGRPGLDPGGGFVDPWGSPYHLVFDADLSGLCEVEDTIYGGQIGIG
                     ILVWSCGPDRRSDTPDDILTWTPSKSEPLEPL"
     gene            complement(10859..11263)
                     /locus_tag="PK634_07155"
     CDS             complement(10859..11263)
                     /locus_tag="PK634_07155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOW97551.1"
                     /translation="MYKQPYRIRMHDTDAAGILFFTSQLRIAHETFEAFLDGVGLGLG
                     VIMGARDYRIPIVHAESDYRAPVHLGDLLSVEVALARVGRTSFALRTRFVSAAGLETG
                     AVTTVQVAVDARTWQKRPLPDELRRALAAGLS"
     gene            complement(11278..11790)
                     /locus_tag="PK634_07160"
     CDS             complement(11278..11790)
                     /locus_tag="PK634_07160"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HOW97552.1"
                     /translation="MNTLAWYYARRGATERCGPVPTRVLRALLTDGRLSPDDLVWRDG
                     LREWIRAGDVPEFQSDGSASPALSPALRRWMTLAGVLSIFSGIGGCLTLFGAATGVLM
                     IIAGSAMLGARTALGSISSADPAWAPFMDQLARYARAMTWALFLGILGTVFFVCFYFG
                     LFAGILSGIP"
     gene            11927..12811
                     /locus_tag="PK634_07165"
     CDS             11927..12811
                     /locus_tag="PK634_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97553.1"
                     /translation="MSDCRSIGRPLATSVILALWAAMMLWLARAKAFPDRFGPAPAGY
                     PDLLAEAPAAEDSWMRLIFRGRTAGYARRRIETQEHDPAAHVVISYQLVARMTVWGVA
                     QDIRASAEASLDAWHQLQGFSLVLRAGTTVFRAEGRRAGASTFDVELSGAAGTSRLTL
                     EIPDEAILYSPLQQLLVKRLSPGQRLNLKAMDPFSFKPSDWTVEAESWENIEIAGRPA
                     RALALRLASGPLSLRAWMDENGRILREESALGWTLEACEAAQALSWAGEGAPGTETEQ
                     DWGGYFDWLRPGDTGPKE"
     gene            12815..13579
                     /locus_tag="PK634_07170"
     CDS             12815..13579
                     /locus_tag="PK634_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW97554.1"
                     /translation="MESMIHVGGISKSFGPVRAVQNLSLDIPRGQLFCFLGPNGAGKT
                     TTIKMLCGLLYPDAGDIRIGGRDLRADPLSVRRITGYIPDFPFLYERLTAAEFFDFSG
                     ELYGLPAVEIRGRREESFVEFGISDYAHTLIKDLSHGYRQRLIYAVTLLHRPEVLFVD
                     EPFIGLDPFTIRLIKNLLREQAGAGMTIFLTTHILALIEDLADRIGIIVGGRLVAAGT
                     RSELERASPAAGGSLEEIFFSLAGPERPAGGTGIHA"
     gene            13572..15239
                     /locus_tag="PK634_07175"
     CDS             13572..15239
                     /locus_tag="PK634_07175"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97555.1"
                     /translation="MRELALLLGKDWRVARHRRRLWSEQSRFKIVFVILFAVGLLGGL
                     WALFLHGFRFLESLGGIGLMLVYRLFYLFFFGLGLMLLLSSVVTTYTTFFRSEETGYL
                     LLKPVPPGTLTLYKAIQSAFYTSWAFFFMIVPFIGAFAMHERLPLRFLAWLVLYSIPF
                     VLLYSLLGTLACLLALPVFRRFRAARWAVPVAAALAAGAWLLKTWPLPGDSSLSPTLT
                     RLVPAMQWASHPLWPSWWMAEGGLALARGKWTDVAMLWGVLASNVLLAGLLVEAAGRR
                     FLLPDWQGAHFAAGHRRRRGVLLPSLDRWLGFLPRDIRTLMIKDARLFLRDPAQWSQA
                     LLFFGLLALYFLNLRTLHYHVLPAAWRNLIAFLNIFSVCSIMASLGSRFVYPQLSLEG
                     HSFWILGLAPTTMGRVLAAKFMASVFGLGAVSLLLMTLSTWMLSVPAAVRVIALLVAL
                     AMTLVVSALSTGLGAVFIDLKQRNPSAIVSGFGGTLNLVLNLGFMLAGILPLGLVFHL
                     RAQPGTDPGGWTAALAAALAWLLLLAGLLAAGALYAGWNSLRRREFG"
     gene            15303..15590
                     /locus_tag="PK634_07180"
     CDS             15303..15590
                     /locus_tag="PK634_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97556.1"
                     /translation="MEDINIDCPFCGQNLDADEDLVGTNVPCPACGKIFRVVQPVRSQ
                     VNAQRIAPPPPPPAPPPNREDMIATTRIELPPEYLSPERKSRIITIKRPGH"
     gene            15669..16457
                     /locus_tag="PK634_07185"
     CDS             15669..16457
                     /locus_tag="PK634_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW97557.1"
                     /translation="MKALTKVGAFAIAKVRSAVTLSAAVWGVLRVALRRSTWPRTSRN
                     VLARQILFTGFEATRFVSLLAVAVGLSIVVQTQMWLARVGQTEFLGPVLVMVVIREAG
                     PLLVNFVIIGRSGTAIATELGNMKIAGEVYLLDAQGLDPFTYLVVPRAVGAAISIFCL
                     TVVFILVSFVSGYVSGWVLQANPGPIDLFVNSVFRAVQPADAGNLFIKTIIPGLTTGA
                     ICCLEGLSVHTAVTEVPQAATRAVVRSTIALFIVSAVVSAVTYL"
     gene            16463..17161
                     /locus_tag="PK634_07190"
     CDS             16463..17161
                     /locus_tag="PK634_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW97558.1"
                     /translation="MDEEKPILELESVGAASGAEHDQGLASLSLALRRGERLLILLDP
                     FERPGLFGDLASGLVEPDAGRVVFDGHDWARLGPARAAAARARIGRVYERPLWVSNLD
                     VDENITLVQRHFTTRPEPEIDAEAVALARSFGLEDLPRLRPARVPPRDLRVADWTRAF
                     LGRRDLLILERPLRDAPPDKAAGLVRAARAASDRGAAVIWLQDAGDRNIVKEFEPARV
                     FEWAGAQWAPVERN"
     gene            17164..18084
                     /locus_tag="PK634_07195"
     CDS             17164..18084
                     /locus_tag="PK634_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOW97559.1"
                     /translation="MAKPFKFRYVNEITGGFVLLTLVLVIVGIILAGNAQRWFEPVHQ
                     LTLTFPPEGSFDLQKGAEVRILGALVGTVESIAVDEAGGMTGEITIRGNFIRFVRVDS
                     KAIAKKKFGVAGDAFVEITKGVGAQLEEGAVLACEKDTELMAMVQDVVQQIRTNTVPA
                     IQQVQKAVEEYTKVAEELHRPEGNLQQLLSHLNGLAAGLEKGEGTAGQLLRDPSLANE
                     LREITAKVNASLQQVSHILANVEQITAALRDETGDMPGLVLQGRETLRESEKLIEGLQ
                     KHWLLRKYVEADEPTARVPASAVIGLEVSP"
     gene            18081..19028
                     /locus_tag="PK634_07200"
     CDS             18081..19028
                     /locus_tag="PK634_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97560.1"
                     /translation="MRRAWFCLAAGLLAAGCSSPPKPARPSDPDLDRYASSAQISFKH
                     GDYAQAEKLYRRALDRARAADDALEIGNNAYNLAVCLVALGRNTEARPLLHEARGALE
                     ELRKNTAEVLLLDASAARNLGILDAASALLLEARAQMSRDKDRALWIQSALVEAQVAC
                     DRNDAPGALRLLDAVSQDLKQLDDPLLKGRNAGLLARVAWMTGQPATAAAWLDKEAGW
                     YRKAGRPPQEISAVLRRAGEAWGQAGQNRESADRYYRAARSLFGQGRANEALKCIGPA
                     LTAAEKAADRGLMARIAALFDEIRREMTAEAADGARAGK"
     gene            19077..19562
                     /locus_tag="PK634_07205"
     CDS             19077..19562
                     /locus_tag="PK634_07205"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOW97561.1"
                     /translation="MRIRSMAWIAGLLALGQAAWAATEMSVQIKSGQIRSTPSYLGQV
                     LATVEYGARVEVLQQRGDWMEVQAAGAPKGWMHSSALTKKKVVLASGSEDVRTGTSGE
                     ELALAGKGFNKEVEAEFKQQNPSVDFKWVDWMEKTRFTIDDLTAFLKAGGVKSAEGGA
                     Q"
     gene            19568..20476
                     /locus_tag="PK634_07210"
     CDS             19568..20476
                     /locus_tag="PK634_07210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOW97562.1"
                     /translation="MLFPVAVALLAAAWWTGCGETVKVAGQLGAAVGVATGSMTTNQA
                     ASLIRSTEAVAKTFEDITPEQEYYIGRAVAATILNKYKPYSNEEAHRYLNLLGQSLAL
                     ASTKPETFGGYHFLILDSNEINAFAAPGGLILVTRGMLRCCRSEDALASVLAHEIGHV
                     QNQHGLKAIKQGRLTSALTILAVESAKSFGSEDLAELTSAFEGSITDITGTLMNSGYA
                     RGQERDADKGAVKILEQVGYGPQALVDMLEQMEQKLKPGGLDFAKTHPDPEDRIRDIR
                     GWIGSAPERAEPAARQARFEKAMQGI"
     gene            20488..>21460
                     /locus_tag="PK634_07215"
     CDS             20488..>21460
                     /locus_tag="PK634_07215"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOW97563.1"
                     /translation="MGKKLLYGILVGLAAALAALGLWRWGKLEPVENIAWRWRVRWLA
                     QPSPETPRIKIILLDQASLDWGKKEMGLSWPWPREMYSAILDFCGRAGARSVAFDVLF
                     TEPSFYGVSDDETFGAAIGRTRAFVAALYIGGETAGDAAAWPEGAPPPWPEFQGLEDW
                     RSGRHAFTGRVVRAAFPVPELVQPGMAWGNVSDQPDMDGVFRRAALFRVFDGRAVPSL
                     GLAAWLADQKATGADVAVRLEPSWLDVGGRRLPIDRRGRATLRYVGRSGTHPSFSAAS
                     IIQSELRLREGGEPVVKPEEFRDAYVFFGFSAPALLDLRPTPISRVYP"
BASE COUNT         3617 a         7138 c         7343 g         3362 t
ORIGIN      
        1 ggcggcgacc gacctggccg cggacaccga cggcgacggg ttcaagaact tcctggccga
       61 tctcgatccg ccgctgttca acacggacga caacagcggg cacccgaacc acgtcgcggg
      121 cggcgaccgg ctcatgcgca tcgccgggag caccaccgac cccctcgagg cggactcgga
      181 cggggatggc ctgccggacg gcatcgagga cgcgaaccgc aacggccggc tggacgcggg
      241 cgagaccgat cccaacaact ccgacaccga cggcgacggc aagctggacg gcacggagga
      301 catcagtcac gacgggctcg tggatggcga ctccaacacc aaccggctct acgacgcggg
      361 cgaggcgtgg ctggagaccg atccgctgaa cgcggacacc gacggcgacg ggctgccgga
      421 cggctgggag aaccggtcgt gcctctccgc gctggacaac ggcacgaaca gcctcgccac
      481 cgcggcggcc ggcgacggaa ccgcggactt cgggcccgcg ggcaacccgg acctcgacgc
      541 gttgagcaat ttgcaggaat acaccggcgg cactgatccg tgctacgcgg acaacggcca
      601 ggcggagccg ggcgaccaga tcacgatcgg cgcgggcaag tcgctcggca cgctggcggg
      661 ggtggacgtg tccgagcagt tcatggactg gacggccgac gactgccaca cgctggacga
      721 ggtccacggc gcgggcaacc tgcgcgagcg cggcgacgta tatttctggg acgacgacta
      781 cgacaccagc cgcgacctgg tggcgttcta ttcccgcgac gggcgcgacc tcaacgagtt
      841 ttacttccgg ctggatttct acgacctgca gtaccaggcc gaggaaggcc acatggattt
      901 ctacgtcgtc atcgacaccg gcaacacgtc cacgggcgag aaggccctgc cggacagcgt
      961 caactgcgcc agcgacatgg gctgggagct ggtggtggcc gtgtacgaga ccgggatcgc
     1021 caacgtgctc atcgaccagg acccgctgct gaacaccgtg aacagctggg acgatctctc
     1081 cgcgcacggc gtcgtgtcgc ggccggactt catacgcggc gtcagtttcc gcgcggacta
     1141 cgacgcggtg gaggtggcca tcgaccgggc cgcgctgatc caggccggct ggaacgggat
     1201 cagcgaactg cactaccagg tttacaccac gcgggacggc acgcaggaca gcccgcgcgg
     1261 gccgggcgac gtcggcggca cgagcgacct cgcggacgcg atcctcgacg acgagcgcgg
     1321 cagcgacgac gccgtgctga acggctggat ttcgcagggc tggaaagcca accgggccaa
     1381 gctggcggtg ctgctgcacg gcaaccagcc cgcggtgccg gcctccgaaa tccagaagca
     1441 gatccgcagc ggtgcgggcg attcggcaac gggctacttc agggcgctgg acgcgaacga
     1501 gttcttcggc ctgccgctga acctgcgcat ctccgggcag ctggcctcga cgatcgagtg
     1561 ggcggccagc gacaccgacc cgcagaagga cgggccctcg ttcaacgcgc gcatccggcg
     1621 gatggtccgc gaaggtcagg ccgcgctgct cgccggcccg tacgcggagc aggcgctgcc
     1681 gttcttcagc tcgacgctga accaggcgtc gctggagatg ggccagctgg cgctgaacac
     1741 gatctacggg acggcgttcc cgaactggtg ggacgtctat ttctaccccg agcgcatgat
     1801 ggacgggccg acgctcacgc aactggccgg gctaggcgcg tatttcggcg tcgtggacca
     1861 gtacacgcac ctctacaact ggttcggccg cgaggcagcc ctcaaggagg aggggttcaa
     1921 gctcaacgag gtcaacgggg tcaagctctt cgcgatcaac cagcaggcgg ccgacgacat
     1981 cttcgtgcgg cacgacggcg gtttgagcct ggggctgcgc ggcctgctct cgaagaaggc
     2041 caacgagtgg tgggcgcggg accaggcggt ggtgatgttc agcctctggg agaacttcgg
     2101 caacacgacc aacgcggcgg cctacgaccg caacctggac tggatcgcca accacccgtg
     2161 gatccaggtc gtcaccctgg agcagatcgc gaagggcgag gtggacatca cgggcgacgg
     2221 ggcgggggat tactggtcca cggtcgcgcg cggcagcggg ctggccttga gcccgcaggc
     2281 caacgactac gtgcatttct ccagcggctc ggacttcggc aactggtacg acggcagcgc
     2341 caacgaggaa tcattgcgcg accggaagtt cacctacgag ggcgccacgc agacggtcaa
     2401 aacgttcggc gacgcgggca cggcggggac gatcctgggc gatgcgtggg ccgcggtggc
     2461 cggcgccggg accagccgcg aggggctgct cgggcggatg gtgctcggcc agggcctgtg
     2521 ggagaccgcg ttccacaacg aggacgaaac cgaccgcagc cggtggagct acggggcgta
     2581 caagcacccg gattccaact acgacacgct tgccggcttc gcgctgaccg cgcaggcgca
     2641 ggcgcggctg ggcgccctgt acggtcgggt ggcgcagtgg gcgacggcgc tgccggccgg
     2701 cgcgaccaac acggccgagg acgtcgatct cgacggcgag agcgaatacg tgctctgcaa
     2761 ccagcgcgtc tttgccgtgt tcgaggacaa cggcgggcgg atgatcgccg cgttcgtccg
     2821 cgatccggcc agcgggcgcg tattccaggg catcggcaac accctcgcct atgccgaggg
     2881 cgagagcgaa gacgagggcg tgagcaacct cgacggatcc gggaacccgc tggcgaagcg
     2941 cacctccgcg ctgaaggact ggtgggccgc cgggccgaac acgaacggct acgtcaacag
     3001 cctctatgcc gcggccgccg tggccggcgg gtggcggctg acctcggccg acgggaagat
     3061 ccagaagacc gtcacgctgg cctccgccac cgccacgacg ttcgaggtgc agtaccaggt
     3121 ccagccctcg atcacggcgc tctacgtgcg gcacggcctg gcgccgaacc tgtacagcct
     3181 ggcgttgaac gggcagaagt acctctccgg cccgactgac gccggcgggc gcgtgaacct
     3241 ggcgcaggac gaccgcgtgg acacggtggc cgtatcggtg ggctacgcgg acgcggggca
     3301 cggcgcggcg tacaacccgg cggcggtgga tcacggcggc gcgttcgccg cgcgcaacct
     3361 gcgcaaccag gcccaggtcc agcaggtcga attctccggc agcggcacgt tcagcttcga
     3421 catccggctc tcggccgggc ggaccgacac cgatggcgac ggcacgccaa acgaatccga
     3481 cacggatgac gacaacgatg gctactccga cacgatcgag cagacggtgg acccgctcac
     3541 cggcgaggcg cagtccgacc cgtggaacgc gggctccgtg ccgccggata tcgacggcga
     3601 tgggatcacg gacgcgctgg atgccgaccg cgacggggat ggcgtcagcg acgacgatga
     3661 attcctggcg ggcaccgatc cccgggacgg caactccgcg ctggtgctgc aagcgatcgt
     3721 caagccggcg ggcgggcggg cgcggatcga gtggccgacc gtcggcggcc ggcgctacac
     3781 ggtggggtat gcgcgcaacg tggcggggca ggggctggtc tggaccgaca tccccgagtc
     3841 ggcgttccag gtctgggaga cggatgtggc cgagggcgag gcggataccg agtccttcac
     3901 ggacgacggc acgctgaccg gcagccctgc gcccgtggcc taccgtgtcc acgcgcatgc
     3961 cccgtgaggg cggaggcatt cgttttgaca gcctgcgacg cgccgtgata gtgtgtcctc
     4021 tgaacaaagt cgttgcggtg ctctatactt cgggccgggg acgatccgct tgacggcgaa
     4081 cgacgaatat attctggaga tcctcgagag cgtcggcctg atcaaccgtg agcaggggcg
     4141 cgccgcgcgg gaggccgccc aggccgagga caagaacgtc atcgagaccc tctccgcctc
     4201 cggcgccgtc tcgaagatgg acatcctgaa ggcgctggcc aaccagttcg ggatggagac
     4261 catcaccctg accggcctgg agatcccgca ggacgtgctg gacatggtgc ccggcgaggt
     4321 ggcccgccgc tacaagatcg tccccgtctt caagaacgac aacatgctga cggtggcctt
     4381 gagcgacccg ctggacgtcg agacgctgga cagcctgcgc tacgtgctga agtgcaacgt
     4441 cgagggcgtg gtcgcgcccg ccgaggaaat cgagacggcc ctcaaccatt actacgcgca
     4501 cagcggcacc acggtggaat ccatgctgga ggagatcacc gagggcacgg tggccatgcc
     4561 caccggcggc cagatcctgg ccggcgaggg agaggtgacc gaggccgacg cgccgatcat
     4621 caagctggtc agcctgatca tcatggaggc gttccgcaac cgcgcgtcgg acatccacct
     4681 cgagccgctg gccaagaaat tccgcgtccg gtaccgcatc gacggcgtgc tccacgaggt
     4741 ggacagcccg ccgaagcgcc tgcagtccgc ggtgatcagc cgcctgaaga tcatggggaa
     4801 catgagcatc gcggagaagc ggctgcccca ggacggccgc atccagatca acgtgatggg
     4861 ccgcgatctc gacctgcgcg tgtcgtccat cccggccagc cacggcgaga gcatcgtcat
     4921 gcgtatcctg gacaagcaga gcctggcgct gggcctgccg cagctcgggt tcttctcgga
     4981 cgaccagcag accttcgagc gcctgatcgg cctttccgac ggcatcctgc tcgtcaccgg
     5041 gcccaccggg tcgggcaaga cgacgacgct ctacgcctgc ctgaactaca tcaaccggcc
     5101 cgaccgcaag atcatcacgg tggaggaccc ggtcgagtac cagatgtcgg gcatcaacca
     5161 ggtgcacgtc cgcacggaca tcgggctgac gttcgcggcg gcgctgcggt ccatcctgcg
     5221 ccaggcgccg aacatcatca tgatcggtga aatccgcgac atggagacag cggagatcgc
     5281 cgtcaacgcg tcgctgacgg gccacctggt gttcagcacg ctgcacacca acgacgcccc
     5341 gagcgcgatc atccgcatga tcgacatcgg cgtgaagccc ttcctcgtcg cctcctccac
     5401 gcgcgcgatc atggcccagc gcctggtgcg caagacctgc gagaagtgca aggagccgta
     5461 caaggccacg gacgcggagc tccggctgct ggggcccgcc gcccaccagc tggcccaggc
     5521 ccagctgttc cggggcaagg gctgctcgga ctgcaacttc accgggtacc ggggccgcct
     5581 gggcatcttc gagatcttca tcatcaacga cgaggtgcgg cacatgatct tcgagcgggt
     5641 ctcggcgacc gagctgcgct cgaaggcgcg cgggctcggg atgcgcacgc tgcgcgagga
     5701 cggcctgcgc aaggtcgtcg cgggcgtcac cacgctgccc gaggtcctgc gcgtgacgat
     5761 gggggatgag ggctgatgag cacgagcgcc tcccagatcg gcatgagcga cctgttgagc
     5821 ctggtcgtca gcgagggggc gtccgacctg cacctcgccg tgggcgtccc gccggtcctc
     5881 cgcctccacg gctcgctgca cccgctcgac gcggatgtcc tcaagccgga ggacaccgag
     5941 cggctgatga agagcatcac ctccgacgag caccagcagc gggtgaagga gcagggcggc
     6001 acggacttcg gcttcgggtt cggcgacctg gcccggttcc gcgtcagcgt gttcaagcag
     6061 aaggggcaca tcggcctggt gctgcgcctg atcccgtcca agctcatgtc gctggaggag
     6121 atcggcctgc cggcccaggt caaggacctg ctcttccggc cgcggggcct gatcctggtg
     6181 accgggccga cgggctcggg caagacgacg accctcgcca gcatgatcaa catcatcaac
     6241 gaggagcgcg actgccacat catcacaatc gaggacccgg tcgagtacta ccactcgcac
     6301 aagaagagcg tgatcaccca gcgcgagatc ggcgtggatg tcccggcgtt caaggaggcc
     6361 ctgcggcgcg gcctgcgcca ggacccggac gtcatcctcg tcggcgaaat gcgcgacctc
     6421 gagaccatgg aggcggcgat taccgcggcg gagacgggcc acctggtctt cgcgaccctg
     6481 cacacgaccg gcgcggcccg tacggtggac cgcatcgtgg acgccttccc gacgaaccag
     6541 caggagcaga tccgcacgca gctcgcgtcc agcatcgtcg ccgtgatctc ccagttgctg
     6601 ctcgtgcgca aggacaagcc cggccgcatc gcggcgttcg agatcatgat cgccacgccg
     6661 tccatccggt cgctgatccg ggacaacaag accttccgga tcacgtcgga tatccagacc
     6721 ggcgcgcgct ggggcatgat gacgctggac gcgcacctga tggccctcta cgagaggaac
     6781 ctgatcggct acgaggactt gatcacgaaa ccgcaggatc ccgaggcggt gtaccagaag
     6841 ctggaggcgt cgggcctccg gccgaggaag taacggccat ggcgaaagcg gactacagcg
     6901 acgcgctgct gcaggcgttg agcgaggagc agatcatcac gtcgcagcag gcggaggaag
     6961 tcctcgacga gcacgagcgc acgggcaagc cggtgcgcga ggtgctcatg gacatggaca
     7021 tggtttccga ggagcgcctg ctggacgtca tcgcggcgca actcggcacg ggcatcgtca
     7081 acctgcacga cctggagatc tcgcaggatg tcgtgcgcac cgtgccgggc agcatcgcgc
     7141 ggatgtacaa cgtggtgccc gtggaactcg gcccgaactc cgtgaccttg gccacctacg
     7201 agttgctgag cccggaggtc gtggacgaac tgcagttcgt gctcacccgg gacgtctcgt
     7261 tcgtggtcgc ccgcgaagag gacatcaaga gctacgtcgg ccgttactac ggcgacgaga
     7321 gcgagtcggt cagcgacatg ctttccgcgc tggaatccga gttgcaggat gcgggggaca
     7381 ttctgaagac gggcgcccgg ggcgatgaca tcgccaatat cgaggcggcc gccagttcca
     7441 cgcccgtggt gcggttcgtg aacctcgtgc tctaccaggc cgtgcaggac cgcgcctcgg
     7501 acatccactt cgagccgttc gagaacgagt tcaagatccg ctaccgcgtg gacggcgcgc
     7561 tgtacgaaat ggccccgccg ccgaagcacc tggccctgcc cgtgacctcg cgcctgaagg
     7621 tcgtggccgg gctgaacatc gccgagcgcc gcatcccgca ggacgggcgc atccagctga
     7681 ccgtcgcggg ccggcccatc gacttccgcg tgtccaccct gccgacgcag ttcggcgaaa
     7741 gcgtggtgct ccgcgtgctc gaccgcagca cggtgtcgct cgacctcgag aacctgggca
     7801 tgccggacga cgtgttccag gccttcctgg aggacatcga caagcccaac ggcatcatca
     7861 tcgtcacggg tccgaccggg tccggcaaaa cgacaacgct gtactccgcc ctgcgccgga
     7921 tcaataccat cgagtccaag ctgctgacgg ccgaggagcc ggtggaatac gacatcgagg
     7981 ggctgatcca gaacccgatc aacgacgcca tcggattgac gtttgcccgg atgctgcgcg
     8041 ccttcctgcg ccaggacccg gacatcatca tggtcggcga ggtgcgcgac atcgagacgg
     8101 cggagatctc catccaggcc tcgctgacgg gccatctcgt gttcactaca ctgcacacga
     8161 acgacgcggc cggcgcgatc acgcggttga tcgacatggg ggtggagccg ttcctgatcg
     8221 cctcgacgct ggaggccgtg ctgggccagc ggctcgtgcg caccatctgc cagaactgca
     8281 agacgcctta ccagcccgag aaggacattc tggaccagct ggggctgagc ctcgaatccg
     8341 tcggaggccg gccgttttat tacggggccg gctgcaccta ctgcaaccag acgggctaca
     8401 agggccggtg cggcatctac gagtacctgc gcgtgcgcga tccagtccgc gcgctgatca
     8461 acgagcgcaa gccgacgctg atcatccgtg acaaggcgat cgagctgggc atgcggacgc
     8521 tgcgcgagga cgggatccgg gcgattttcg acgggcagac caccgtcgag gaagtactga
     8581 agtacaccta gccgggggag agaggcaacc cgatcgcggg gggagagagg accatggcga
     8641 aattttcata cacggcgctg gacgccaagg gacgcgagac ccagggcgag atcgaggccg
     8701 acaaccagac ctccgccttg agcaagatcc gcgagaaggg cctgttcccg acccaggtcg
     8761 cggacgccgc ggccaagccc gccaagaaga aggccacgaa gggcgccggg ggtatgatga
     8821 agatggagct gcggatgccc gcgttcctct cccgcgtgaa gaccaagcag ctcatggtct
     8881 ggacccgcca gttggccacg ctggtcaacg ccgggctgcc cctgctgcgc ggcctccgcg
     8941 tgctgaccaa gcaggagaag aatcccctgc tcaagcgcac ggtgtccgag atggccgagt
     9001 ccatcgagtc gggcagcacc ttcgccgagg ctctggcgca gcacccgcgc atcttcaaca
     9061 agctgttcgt caacatggtc aaggccggcg aggtcggcgg cgtgctggac gtcgtgctcc
     9121 tgcggctggc cgaattcatg gagaaggcgc agaagatcaa gagcaaggtt gtcagcgcca
     9181 tggtctaccc ggtggtcgtg ctcatcatgg ccggcagtat cctgacgttc ctcatgatct
     9241 tcatcgtccc caagttccag gacatcttcg ccgacctgtt ggagggcgag caactccccg
     9301 gcctgacgcg catggtcctc tccgtcagca agaccatgac cagccgcctg cccatcgtcg
     9361 tcgggatcgt cgtgatcctc gtggtcctgg ccaagatcct cgcgaaagcg cgtttcgggc
     9421 ggctggtcat ggacaacctc aagctgaaga tgcccatttt cggcacgctg gtccgaaaga
     9481 ccgccatcgc ccggttcacc cgcacgctgg gcacgctcat gacctccggc gtgccggtcc
     9541 tgcaggccct gaacatcgtc cgcgacaccg tccagaacga ggtcatcagc aaggccgtcg
     9601 gcctgatcca cgacagcgtg aaggaaggcg aaaacatgac gcggcccatc gaggcgtgcg
     9661 gcgtgttccc tccgatggtg gtcagcatgg tggaggtcgg cgaggagacc ggcggcttgc
     9721 ccgaaatgct gatgaagatc gcggacagct acgacgacga cgtggataac accgtggtcg
     9781 gcctgacgtc tatcatcgag ccgatcctga tcatattcct ggccatcatc gtcggcacca
     9841 tcgtcattgc gctgttcctg cccctgatct cgatcatcgg caagatgagc tgacgggcgg
     9901 cgggccggca gggccgtgac catgaccggg cgaagaataa ggccgtcgac agccatcgcc
     9961 gcgggcatcg cgctgttgtt ggtttcatgg attgttccgg gtttctggca tgcgcgaagg
    10021 caacgtgccc aggcctacgc ccgggcgggc gcgcgggcgc tccagcaggc cgcgcagcat
    10081 ttctactccg attacggggt atggcccgtg gatcagcgag tgtccgtccg ggatatccat
    10141 ttcggagacg cgcggccgaa tgccgaggtc ctgaacgctc tccgcggtgt cgcgggtccg
    10201 ggcaacgaag accatgccgt gaatccgcat cgcacggtgt acttcgatcc gctggccgcg
    10261 ggcccgggtc gtcccggatt ggacccgggg gggggattcg tggatccgtg gggcagtccc
    10321 tatcatcttg tgttcgatgc cgacttgagt gggttgtgcg aagtcgagga cacgatatac
    10381 gggggacaga tcgggatcgg catccttgtg tggtcctgcg ggcccgaccg gcgctcggac
    10441 acgccggacg acatcttgac ctggacgccg tccaagtcgg agccgctgga acccctctag
    10501 tgcttcgcgt aaacggcagt cgtcccatct cgcgacatgc cgcaggtctt cattgatgga
    10561 aagagctcga cctggacagc tgcggcccta ataggggaag acgcctatct ccaggcaaac
    10621 gcggaatatc acagtttata aactgtgata catgcctttt gagggcaaaa ttcatcataa
    10681 ccattaggaa atacgcacat cgtgaaatac tagatatcac agtttataaa ctgtgataca
    10741 cgggcttccc ggtgcatttc ctgagcggcg acgatctcgt accgcgatgg attgcttgta
    10801 atatggagac gaattgttgt tgcttcgacg tcgcttggcc accagagagt gcgcggccct
    10861 aactgagtcc agcggccagg gcgcggcgca gttcatcggg cagggggcgt ttttgccacg
    10921 tgcgggcgtc cacggccacc tgcaccgtcg tcaccgcgcc ggtctccagg ccggcggcac
    10981 tcacgaatcg ggtgcgcagc gcgaaggacg tgcggcccac ccgcgccagg gccacttcca
    11041 cggaaagcag atcgcccagg tgcacgggcg cgcggtagtc ggactccgcg tgcacgatcg
    11101 ggatccgata atcccgcgcc cccatgatca cacccagccc gagtccgacc ccgtccagaa
    11161 aggcttcgaa cgtctcgtgc gcgatgcgca gctggctggt gaaaaagaga atgccggcgg
    11221 catccgtgtc gtgcatgcgg atgcgatagg gttgtttgta catggcgggc cttcaggtca
    11281 ggggatgccc gacaggatgc cggcaaagag gccgaaatag aagcagacga agaacaccgt
    11341 gcccaggatg cccaggaaga gggcccaggt catggcccgg gcatagcgcg ccaactggtc
    11401 catgaacggc gcccacgcgg gatcggcaga ggatatggat cccagcgccg tccgggcgcc
    11461 cagcatggcg ctcccggcaa tgatcatcag cacgccggtg gccgcgccga agagcgtgag
    11521 gcagccgcct atgccgctga agatcgacag caccccggcc agcgtcatcc aacgacgaag
    11581 ggcaggggac agggccgggg aggcgctccc gtccgactgg aattccggca cgtctcccgc
    11641 ccggatccat tcccgcaggc catcgcgcca gaccaggtca tcgggcgaga gacggccatc
    11701 ggtcagcagg gcccgcagca cccgcgtggg caccggcccg caacgctccg tggcgccgcg
    11761 gcgggcgtag taccatgcaa gagtgttcat cgttcgtcgt gtttcactat aagccatgcg
    11821 caggcccatg ccacatcttt tcaatacgcc ggggcatgac cggtcggagt cactttgccc
    11881 tgttcaagcg gttcgcggcg cgggtatgtt cagttcccgt tgcgacatga gcgattgccg
    11941 atccataggg cggcccctgg cgacatcggt catccttgcg ctctgggcgg ccatgatgct
    12001 ctggctggcc cgggccaagg cgttccccga tcgcttcggc ccggcccccg ccggctaccc
    12061 cgatctcctc gccgaagcgc ccgccgccga ggactcatgg atgcggctga tctttcgcgg
    12121 caggacggca ggctatgccc ggcgacggat cgaaacacag gagcacgatc cggcagccca
    12181 cgtggtgatc agctaccagc tggttgcgcg gatgacggtc tggggcgtcg cgcaggatat
    12241 ccgggcctcg gccgaggcga gcctggacgc gtggcatcag ttgcagggat tcagtctcgt
    12301 cctgcgagcc ggaacgaccg tattccgtgc ggagggccgg cgagccggcg cctcgacgtt
    12361 tgacgtggaa ctgagcgggg ccgcgggcac gtcgcgcttg acgctcgaaa tcccggacga
    12421 ggccatcctc tattcccccc tgcagcagtt gctggtcaag cggctgtcgc ccggccagcg
    12481 gctgaacttg aaagccatgg atcccttctc gttcaagccg tcggactgga cggtggaagc
    12541 cgagtcctgg gagaacatcg aaatcgcggg ccgacccgcc cgggcgctgg cgctccggct
    12601 cgcctcgggc ccgctttccc tccgcgcctg gatggacgag aacgggcgca tcctgcgcga
    12661 ggagtccgcg ctgggctgga cactcgaggc ctgcgaggcg gcacaggccc tgtcctgggc
    12721 cggtgaagga gcgcccggca cggagaccga acaggactgg ggcgggtatt ttgactggct
    12781 gcggcccggg gacacgggcc cgaaggagta ggcgatggaa tcgatgattc acgtgggcgg
    12841 aatttcgaag tcgttcggcc cggtgcgcgc ggtgcagaat ctgtcccttg atatcccgcg
    12901 gggccagttg ttctgcttcc tcgggcctaa cggcgccggc aaaacgacca cgatcaagat
    12961 gctgtgcggc ctgctgtatc ccgacgcagg cgacatccgc atcggcggcc gcgacctccg
    13021 cgcggacccg ctctccgtgc ggcggatcac cgggtacatc cccgatttcc ccttcctgta
    13081 cgagcggctg accgcggccg agttctttga tttttccgga gagctctacg gcctgcccgc
    13141 tgtcgagatc cgcgggcgcc gggaggagtc gttcgttgag ttcggcattt cggattatgc
    13201 tcatacgctg atcaaggatc tgtcccacgg ctaccggcag cgcctgatct acgccgtcac
    13261 gctgctgcac cggcccgagg tcctgtttgt cgacgagccg ttcatcgggc tggatccatt
    13321 caccatccgg ctgatcaaaa acctgctccg cgagcaggcg ggggcgggca tgacgatctt
    13381 cctgaccacg cacatcctgg cgctgatcga ggacctggcc gaccgcatcg ggatcatcgt
    13441 cggcgggcgt ctcgtggcgg ccggtacccg ttccgaactg gagcgggcga gcccggcagc
    13501 gggcggcagc ctggaggaga tctttttcag cctggcgggg cccgagcgcc cggccggcgg
    13561 gacgggtatc catgcgtgag ctggccctgc tgctcggcaa ggactggcgg gtggcgcgcc
    13621 accgccggcg cctgtggtcg gagcagtccc gcttcaagat cgtcttcgtc atcctgttcg
    13681 cggtcgggct gctcggaggc ctctgggcgt tgttcctgca cgggttccgg ttcctggagt
    13741 cgctcggcgg gatcggcctc atgctggtct accggctgtt ctacctgttt tttttcgggc
    13801 tgggcctgat gctgctgctc tcctcggtcg tgaccacgta caccacgttc ttccggtcgg
    13861 aggagaccgg ctatctcctt ctcaagcccg tgccgccggg gacgctgacc ctgtacaagg
    13921 ccatccagtc cgccttctac acctcgtggg cgttcttctt catgatcgtg cccttcatcg
    13981 gcgccttcgc catgcacgaa cggctgccgc tccggttcct cgcctggctc gtgctgtact
    14041 ccatcccgtt cgtgctgctc tactccctgc tggggacgct ggcctgcctg ctggcgctgc
    14101 ccgtgttccg gcgtttccgc gcggcgcgct gggcggtccc cgtcgcggcg gcgctggccg
    14161 ccggcgcgtg gctgctgaag acgtggccgc tgccggggga cagcagcctg tccccgacgc
    14221 tgacgcggct cgtgcccgcc atgcaatggg cttcgcatcc gctgtggccg agctggtgga
    14281 tggccgaggg gggactggcc ctggcccgcg ggaagtggac cgacgtggcg atgctctggg
    14341 gcgtgctggc gtcgaacgtg ctgctggccg ggctgctcgt cgaggccgcc ggccgtcggt
    14401 tcctgctgcc cgactggcag ggcgcgcatt tcgccgccgg ccaccgccgc cgccgcgggg
    14461 tcctgctgcc gtcgctggac cggtggctgg gcttcctgcc gcgcgatatc cggaccctga
    14521 tgatcaagga tgcccgcctc ttcctgcgcg atccggccca atggtcccag gcgctgcttt
    14581 ttttcggcct gctggcgctg tacttcctga acctgcgcac gctgcactac cacgtgctgc
    14641 ccgccgcctg gcgcaacctg atcgccttcc tcaatatctt cagcgtgtgt tccatcatgg
    14701 cctcgctggg ctcccggttc gtctacccgc agttgagcct cgagggacac agcttctgga
    14761 tcctcggcct ggccccgacc acgatgggcc gcgtgctcgc cgccaagttc atggcgtccg
    14821 tcttcggcct cggcgccgta agcctcctgc tgatgacgct ctccacatgg atgctgtccg
    14881 tcccggccgc ggtgcgggtc atcgcgctgc tggtcgcgct ggccatgacc ctcgtggtgt
    14941 ccgcgctctc caccgggctc ggcgcggtct tcatcgatct gaagcagcgg aacccgtccg
    15001 ccatcgtctc cgggttcggg ggcaccctga acctggtgct gaacctcggc ttcatgctgg
    15061 ccggcatcct gccgctcggc ctcgtcttcc acctgcgggc ccagccgggc acggatccgg
    15121 ggggctggac ggcggcgctg gccgccgcgc tggcctggct gctcctcctt gccggcctgc
    15181 tggcggcggg cgccctgtac gccggctgga acagcctgcg ccgccgggaa ttcggctgac
    15241 cggtcaattt tgcttcacaa gctttccgcg cagggatacg attgacactg gaggcagcgg
    15301 acatggaaga cattaacatc gattgcccct tttgcggtca gaacctggac gccgacgagg
    15361 acctggtcgg aaccaacgtg ccgtgtcctg cctgcggaaa aatcttccgc gtcgttcagc
    15421 ccgtacggtc ccaggtgaac gcccagcgaa tcgcgccgcc gcctccgccg ccggccccgc
    15481 ccccgaaccg cgaggacatg attgccacga cgcggatcga actgcccccc gagtacctct
    15541 ctcccgagcg gaagagccgg atcatcacca tcaagcggcc cggccactga tcccgcagcc
    15601 gtccctttcg ggcttggcgc ggcggcgcgc ttttgagaaa ttctgcccgc cgggaatcga
    15661 aggcattcat gaaggccctg actaaagtcg gagcgtttgc catcgccaag gtccgctcgg
    15721 cggtcaccct gtcggccgcc gtctggggcg tgttgcgggt cgccctccgg cgctccacct
    15781 ggccccgcac ctcccgcaac gtgctggccc ggcagatcct tttcaccggg ttcgaggcca
    15841 cccgattcgt atccctgctt gccgtggccg tcggcctctc gatcgtggtc cagacccaga
    15901 tgtggctcgc gcgggtgggg cagaccgagt ttctggggcc ggtcctggtc atggtcgtga
    15961 tccgcgaggc ggggccgctg ctggtcaact tcgtcatcat cggccgcagc ggcacggcca
    16021 tcgcgacgga actcgggaac atgaagatcg ccggggaggt ctacctgctg gatgcccagg
    16081 ggctcgaccc cttcacctac ctcgtcgtgc cgcgcgccgt gggcgcggcg atttcgatct
    16141 tctgcctgac cgtcgtcttc atcctggtct ccttcgtcag cggctatgtc agcggctggg
    16201 tgctgcaggc caatcccggc ccgatcgatc tcttcgtcaa cagcgtcttc cgggccgtgc
    16261 agcccgcgga cgccggcaac ctgttcatca agaccatcat tcccggcctg accacgggcg
    16321 ccatctgctg cctcgagggg ttgagcgtgc atacggccgt caccgaggtg ccccaggcgg
    16381 ccacgcgggc cgtggtgcgc tccaccatcg ccctgttcat cgtgtcggcc gtggtctccg
    16441 cggtgaccta cctgtgagcg tcatggacga agagaaaccc atcctggaac tcgagagcgt
    16501 cggcgccgcg tccggcgcgg agcacgatca gggcctggcc tcgctctcgc tggccctccg
    16561 gcgcggcgag aggctgctga tcctgctgga tcccttcgaa cgcccgggcc tttttggcga
    16621 cctggcctcg ggcctggtcg agcccgacgc cggccgggtt gtcttcgacg gtcacgactg
    16681 ggcccgcctg ggcccggcgc gggcggccgc cgcccgcgcg cgcatcggcc gcgtgtacga
    16741 gcggcccctc tgggtcagca acctggacgt ggacgagaac atcacgctgg tccagcgcca
    16801 tttcaccacc cggccggagc ccgagatcga cgccgaggcg gtggccctgg cccgctcgtt
    16861 cggcctcgag gatctgcccc ggctgcggcc ggcccgggtg ccgccgcgcg acctgcgcgt
    16921 ggcggattgg acccgggcgt tcctgggccg gcgggacctg ctgatcctgg agcgccccct
    16981 gcgcgatgcg ccgccggaca aggccgccgg cctcgtccgg gccgctcggg cggccagcga
    17041 ccggggcgcg gcggtaatct ggttgcagga cgccggggac cggaatatcg tcaaggagtt
    17101 cgagccggcg cgcgtgttcg aatgggccgg cgcgcaatgg gcgccggtgg aaaggaacta
    17161 agcatggcca agcccttcaa gttccgctac gtgaacgaga tcacgggcgg gttcgtcctc
    17221 ctgaccctcg ttctcgtgat cgtcggcatc atcctggccg ggaacgccca gcgctggttc
    17281 gagcccgtcc accagctcac cctgaccttt ccgcccgaag gctcgttcga cctccagaag
    17341 ggggccgagg tccggatcct cggggcgctg gtcggcaccg tggaatccat cgccgtggac
    17401 gaggcgggcg gcatgaccgg cgaaatcacc atccgcggca atttcatccg gttcgtgcgc
    17461 gtcgactcga aggcgatcgc gaagaagaag ttcggggtgg ccggcgacgc gttcgtggag
    17521 atcaccaagg gcgtcggggc gcagctcgag gagggcgccg tgctggcctg cgagaaggac
    17581 accgaactga tggccatggt ccaggacgtg gtgcagcaga tccgcacgaa cactgtcccg
    17641 gcgatccagc aggtgcagaa ggccgtggag gaatacacca aggtcgccga ggaactgcac
    17701 cgcccggaag gcaacctgca gcagttgctc tcgcacctga acggcctggc cgccggcctg
    17761 gagaagggcg agggcacggc cggccagctg ctccgggacc cgtccctggc caacgagctg
    17821 cgcgagatca ccgcgaaggt gaacgcctcg ttgcagcagg tcagccacat cctggccaac
    17881 gtggagcaga tcaccgccgc cctccgcgac gagacgggcg acatgccggg gctggtcctc
    17941 cagggccgcg agacgctgcg cgaatccgag aagctgatcg aggggctcca gaaacactgg
    18001 cttctgcgca agtacgtgga ggccgatgag cctaccgcgc gcgtcccggc ctcggccgtg
    18061 atcggcctgg aggtgtcgcc atgaggcggg cgtggttctg tctcgcggcg ggcctgctcg
    18121 cggcggggtg ctcctcgccg ccgaagcccg cgcgcccctc cgacccggac ctcgatcggt
    18181 acgcgagttc ggcccagatt tccttcaagc atggcgacta cgcgcaggcc gagaaattgt
    18241 accgccgcgc tttggaccgc gcgagggcgg ccgacgatgc cctggaaatc ggcaacaacg
    18301 cctacaacct ggcggtgtgc ctggtcgccc tgggccggaa cacggaggcc cgcccattgc
    18361 tccacgaggc gcgcggggcg ctggaggaac tccggaagaa cacggcggag gtgctcctcc
    18421 tggatgcctc ggcggcccgg aacctcggga tactggatgc cgcctcggcc cttctgctcg
    18481 aggctcgcgc ccagatgagc cgcgacaagg accgggcgct gtggatccag tcggccctgg
    18541 tcgaagccca ggtggcctgt gaccggaacg acgcgcccgg ggcgcttcgc ctcctcgatg
    18601 ccgtgtcgca ggacctcaag cagctcgacg acccgctgct caagggccgg aacgccggcc
    18661 tgctggcccg cgtcgcatgg atgaccggtc agccggccac ggccgcggca tggctggaca
    18721 aggaagcggg ctggtaccgg aaggccggtc ggccgcctca ggagatatcc gccgtgcttc
    18781 gccgcgcggg cgaggcgtgg ggacaggccg gccagaaccg ggagtcggcg gatcgctact
    18841 accgggcggc gcgcagcctg ttcggacagg gaagggccaa cgaggcgctg aagtgcatcg
    18901 gcccggccct aacggcggcg gaaaaagccg ccgatcgcgg gctgatggcc cgaattgccg
    18961 ccctttttga tgaaatcagg cgggaaatga ccgccgaggc ggcggacggc gcccgagccg
    19021 gtaaataaac ggggcgcccg gccgtctaat ggatggaagc ggaaaggaat ccggacatga
    19081 gaatacgaag catggcatgg atcgcggggc tgctggccct tggccaggcg gcttgggccg
    19141 cgaccgagat gagcgttcag atcaagtcgg ggcaaattcg ctccaccccg tcctacctgg
    19201 gccaggtcct ggccacggtg gaatacggcg cgcgcgtcga ggtgctgcag cagcgcggcg
    19261 actggatgga ggttcaggcg gcaggcgccc cgaaagggtg gatgcactcg tcggcgctca
    19321 cgaagaagaa ggtggtgctg gcttccggtt ccgaggatgt ccggaccggg acctcgggcg
    19381 aagaactggc cctggccgga aagggattca acaaggaagt ggaggcggag ttcaagcagc
    19441 agaacccgag cgtggatttc aagtgggtgg actggatgga gaaaacccgc ttcaccattg
    19501 acgatctcac ggccttcctc aaggcgggcg gcgtgaagag cgccgaagga ggcgcccagt
    19561 gaagaagatg cttttccctg tggcggtcgc gctgctcgcg gcggcatggt ggaccgggtg
    19621 cggcgaaacc gtgaaggtgg ccggccagtt gggcgccgcg gtcggcgtgg cgaccggcag
    19681 catgacgacc aaccaggccg cctcgctcat ccgctcgacc gaggcggtgg ccaagacctt
    19741 cgaggacatc acgcccgagc aggagtacta catcggccgc gcggtggccg cgaccatcct
    19801 caacaagtac aagccctatt cgaacgagga ggcgcaccgc tacctcaacc tgctcggcca
    19861 gagcctggcg ctggcctcga ccaagcccga aaccttcggc gggtatcatt tcctgatcct
    19921 cgacagcaac gagatcaacg ccttcgcggc gccgggcggg ctgatcctgg tcacgcgggg
    19981 catgctccgc tgctgccgct ccgaggacgc gctggcctcc gtgctggccc acgagatcgg
    20041 ccatgtccag aaccagcacg gcctgaaggc gatcaagcag ggccggctga cctccgccct
    20101 caccatcctc gccgtcgaga gcgccaagag tttcggcagc gaggacctgg cggaattgac
    20161 ctcggccttc gagggcagca tcaccgacat caccggcacg ctgatgaaca gcggctatgc
    20221 ccgcggccag gaacgcgacg cggacaaggg cgccgtgaag atcctggagc aggtcggtta
    20281 cggcccgcag gcgctggtgg acatgctcga gcagatggag cagaagctga agcccggcgg
    20341 gctcgatttc gccaagacgc acccggaccc ggaggaccgg atccgggaca tccgcgggtg
    20401 gatcggttcg gcgccggagc gggccgagcc ggccgcgcgc caggcccggt ttgagaaggc
    20461 gatgcagggc atctaggcgc gccggtcatg gggaagaagc ttctctacgg gattctcgtc
    20521 ggcctggccg cggcgctggc ggcgctcgga ctgtggcgct ggggtaagct cgaacccgtg
    20581 gagaacatcg cctggcggtg gcgcgtccgc tggcttgccc aaccgagccc ggagacgccg
    20641 cggatcaaga tcatcctgct cgaccaggcc agcctcgact ggggcaagaa ggagatgggg
    20701 ttgtcgtggc cttggccgcg cgagatgtat tccgcgatcc tggatttctg cggccgggcc
    20761 ggcgcccgtt ccgtggcctt cgacgtgctc ttcaccgagc cgtcgttcta cggcgtgagc
    20821 gacgacgaga ccttcggggc cgccatcggg cggacgcgcg cgttcgtcgc cgctctgtac
    20881 atcggcggcg agacggcggg cgacgccgcc gcatggccgg aaggcgcgcc gccgccctgg
    20941 ccggaattcc agggcctgga ggactggcgg tccggccggc acgccttcac gggccgggtc
    21001 gtccgcgccg ccttcccggt cccggaattg gtccagccgg gcatggcctg gggcaatgtc
    21061 tccgaccagc ctgacatgga tggcgtgttc cggcgcgcgg cgctcttccg ggtgttcgac
    21121 gggcgggccg tcccgtcgct gggcctcgcc gcgtggctgg ccgaccagaa ggcaaccggc
    21181 gccgacgtgg cggtgcgcct ggagcccagt tggctggacg tgggcgggcg gcggctcccg
    21241 atagaccggc gcggacgcgc cacgctgcgc tacgtcggac ggtccggcac gcatccttcg
    21301 ttcagcgcgg cctcgatcat ccagtcggaa ctccgcctgc gcgagggcgg ggagcccgtc
    21361 gtgaagccgg aggaattccg cgacgcctac gtcttcttcg gattcagcgc cccggccctg
    21421 ctcgacctgc ggcccacgcc gatcagccgc gtctatcccg
//
LOCUS       DAOOTK010000048        21187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1726_length_21187_cov_5.555130, whole genome shotgun sequence.
ACCESSION   DAOOTK010000048 DAOOTK010000000
VERSION     DAOOTK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21187
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_21187_cov_5.555130"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK634_07220"
     CDS             <1..480
                     /locus_tag="PK634_07220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903443.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOW97564.1"
                     /translation="EGGWTRLLPGGESLPIYDAAMRYAGAGTPLVVLAGKMYGAGSSR
                     DWAAKGTRMLGVRAVIAESFERIHRSNLVEMGVLPLEFTGGQNAEKLGLTGREEIAIA
                     GIAGGLAPGKLLDVDAGGASFRVQCRIDSAIEVEYYRQGGILPYVLRQAMGTHSSGS"
     gene            565..1392
                     /gene="thyX"
                     /locus_tag="PK634_07225"
     CDS             565..1392
                     /gene="thyX"
                     /locus_tag="PK634_07225"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOW97565.1"
                     /translation="MAHTYSPEAEKLLDQEIKVLDQGFVRLVDYMGSDARIVQTARVS
                     YGAGTKTVREDAGLIDYLLRHEHTSPFEHVVIELHCKLPIFVARQWIRHRTARLNEIS
                     ARYSVMADEFYLPDEAHINAQSADNKQGRSDAEVPPELRRKVLELLQRDQGMAYASYE
                     EMIKDGIARELARINLPLSLYTQWYWQMDLHNLFRFLELRMDSHAQWEIRQYAAAIAQ
                     IARAVAPVAFDAFERHRLKGRHFSADELDALRRMLKGEENPLTGSRRREFERKLTAT"
     gene            complement(1406..2068)
                     /locus_tag="PK634_07230"
     CDS             complement(1406..2068)
                     /locus_tag="PK634_07230"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOW97566.1"
                     /translation="MNWKQPIGKLYRHAPAIRAALLVFGSALLLAQLALGGWRSARSS
                     DAELADHQILSWSLEAAELSPVWVPEDCLVEAVIQVESGGDPRKVGKAGERGLMQIKR
                     GTWRDVTRRLYGKPVSFDRAFEPELNRIVGRAYLAHLQDFLGRNQSHWKTDELSLLLA
                     CYNAGPTRVAKGGFHPDRWPVLTREYVQRVTALHEHYLAEGPPSARAVMAQVPAPRPP
                     GS"
     gene            complement(2301..2591)
                     /locus_tag="PK634_07235"
     CDS             complement(2301..2591)
                     /locus_tag="PK634_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97567.1"
                     /translation="MISRICDRFGQPLETGGLRYIARIQVYAAYDPLEITFDDLGRDY
                     SAEIRRIIEECRDLTEEELMRDVYVDFSFDLCPACQKAYIRDPLRASEPGNG"
     gene            2699..2872
                     /locus_tag="PK634_07240"
     CDS             2699..2872
                     /locus_tag="PK634_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOW97568.1"
                     /translation="MTTASDGKWVCTCCGYVYDPATGDAALGLPAGVPFEQLPENWKC
                     PICYAAKDAFDPL"
     gene            complement(3035..3628)
                     /locus_tag="PK634_07245"
     CDS             complement(3035..3628)
                     /locus_tag="PK634_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97569.1"
                     /translation="MARVALKQLSVAQLQGELKRRQGALPRLEKKRKKLAAALAKVEE
                     AIADLGVVVSRIKGTGKAQPRRGRPAKRGRPAKRGRPAKRGRPAGKARRVKNAQSLGD
                     VLVKVLTGIKSMKVAEAAEAVAKTGYKSKAKNFKLIVNQTLVKDKRFRSVRRGRYALS
                     RAGKPAAAKPAAKKARVKKAAAKKAPAPKTPVAPPAG"
     gene            3766..4374
                     /locus_tag="PK634_07250"
     CDS             3766..4374
                     /locus_tag="PK634_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW97570.1"
                     /translation="MAAEHGILDAQAAAWLDEYRQGRVEALGRVVEQYRRPLFGFILN
                     MTEGREDAEEVFQETWRRALRHADRLDGRRLLSWLFRVAHNLIVDRVRAKKPVESLNS
                     EPRAEEPGRAPLADRLPAPGPTPAGESAGRDLDRRIRQAIDRLPPEQREVFLMRIEGD
                     VPFREIARLQEVSINTALARMQYALKKLREDLKDEYAAWVEE"
     gene            4377..4922
                     /locus_tag="PK634_07255"
     CDS             4377..4922
                     /locus_tag="PK634_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97571.1"
                     /translation="MNCETTRNRILLDQSRELSRVGRWLLAGHLARCPECRAYRAGLT
                     GLAGAVRQVRWDESFDAGLAGRVAAGAAVPAPRAKRGRERAPVFLARPALAYGALSVL
                     LALAFVLILRPFQRPPPLARTAPDSALAWDADVDDRLATLDVLVNQDDADWTETSGAA
                     ADSGDVDSIARQLLELEGEQI"
     gene            4919..5620
                     /locus_tag="PK634_07260"
     CDS             4919..5620
                     /locus_tag="PK634_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97572.1"
                     /translation="MKSIHRRFLTAGIIAGLAFAIRAAAQDFDEASPPPPEAPDLAEP
                     PGPPPGEAGPVDRWMERLEKRNPDEYLRYREMREKDPEGFRRELAGRLGRERLQTVMN
                     AHPRLRVFLDSLPESERMDVLQALGRALRPGGPPHEPNAGELRPLHEEVARLAREYRQ
                     STDQAQREQRRADLKLKLGELFDAREQGRADHIARIQAEVAKLQQALDSRRAQRDEIV
                     ERRLQELTADESSKW"
     gene            complement(5652..5963)
                     /gene="rsfS"
                     /locus_tag="PK634_07265"
     CDS             complement(5652..5963)
                     /gene="rsfS"
                     /locus_tag="PK634_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOW97573.1"
                     /translation="MAREVLSDRKGEDPVLLDMRGLSGVTDYYLVVTGRNAPHLKAMA
                     DELERALSRGGARCFRRAGAPESGWVVADYLDAVIHLFSADARSYYALEKLWGDAPRV
                     R"
     gene            complement(6000..6611)
                     /gene="nadD"
                     /locus_tag="PK634_07270"
     CDS             complement(6000..6611)
                     /gene="nadD"
                     /locus_tag="PK634_07270"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOW97574.1"
                     /translation="MSDRLGILGGTFNPVHLGHLMMAQDALERFRLDRVIFMPCARPP
                     HKAARDLAPDADRLAMLRRAVRGDPRFTVSDLEIRRGGISYTIDTIKALQRNHPRARL
                     HFIIGSDSLFELHAWREVGELLRRCVFIALERPGYSLARMTARRLRLPEPWPRRLART
                     VFRGHVMEISSTEIRARAAAGLSLRYLVAPAVAGYIRSKKLYR"
     gene            complement(6608..7384)
                     /locus_tag="PK634_07275"
     CDS             complement(6608..7384)
                     /locus_tag="PK634_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97575.1"
                     /translation="MKKMGTILLGIVVVVAILIALLFFATSGLPKAADEFFSLVREGK
                     VGEAYLATAAEFRAATGEEEFSAFLRDSALGQYASASWPTRAIENNRGRLEGTVTTRD
                     GGQIPVELDLVKEGGAWKILNLRKAAAGLAQGGASTVEIPPDAELQRMTSEAIGLLAE
                     AINKNDFSGFRAHISQLWQSQVTEEQLREAFKEFAEKQIDLAPLKSQTPVFSQKPAVD
                     ENGALNLRGYYATTPDQVRFELSFIYEHPQWKLSSIGLHL"
     gene            7663..9099
                     /gene="purF"
                     /locus_tag="PK634_07280"
     CDS             7663..9099
                     /gene="purF"
                     /locus_tag="PK634_07280"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOW97576.1"
                     /translation="MNNRNGKLDLAEPREACGLFGVFGVPETARAIHAGLFALQHRGQ
                     EGAGIVVSNGEKIRSIKGMGLVAEVFATRSLAELEGHLGIGHVRYSTTGSSRPQNVQP
                     IVFECVDGLWALAHNGNLTNSQAMRRSYQEAGAIFQTSTDSEILMHLIADPAYRGRPR
                     RVERALAELQGAFSFLLMTRDSVLAARDRYGFRPLSVGRLGGGYVFASETCALEQVGA
                     TFERDVLPGELVVVDAKGIRGSTFCEPVSGPLGQCIFEHVYFARPDSTIFGQSVHETR
                     VALGRQLAREHPVEANLVMPVPDSGSSAALGYSLESGLPMDYGFIRNHYIGRTFIMPE
                     SDQRSGQVDLKLAVVPGVVRDKRVVVVDDSIVRGTTSRRRIAALRKAGAREIHLRISC
                     PPIRHPCYFGIDFPSRKDLAAAQHPLEDIRRFIGADSLAFLRLEGLLAAFEQPGDYCT
                     GCFSGKYPAPVPEQHSKTALENAQAAPA"
     gene            9096..13676
                     /gene="gltB"
                     /locus_tag="PK634_07285"
     CDS             9096..13676
                     /gene="gltB"
                     /locus_tag="PK634_07285"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOW97577.1"
                     /translation="MNPLPPAAQGLYHPRHEHDACGVGFVCHMRGEKSHDVIHKALRI
                     LVNLTHRGACGCDEKTGDGAGILMQFPHAFFAGRAPEAGLKLPGEREYGAGLVFLPRD
                     ARERGHCVRLFGDAIRAEGQVLLGWRNVPVHNEGIGDLARQVEPFIAQVFIGRGPGLD
                     DEAHFERKLFVIRKVMERAVRESDLREKKYFYIPSLSSQTFVYKGLLLADQIEPFFPD
                     LTDTAMVSGLALVHQRYSTNTFPTWDLAQPFRFLCHNGEINTLRGNINWMNTRQYLFQ
                     STRFGDDMAKLFPIATPGASDSAILDNAVELLYHTGRPLPQAVMMLIPEAWQNHRTMP
                     DEKKAFYEYHANLMEPWDGPASIPFTDGRCMGAVLDRNGLRPSRYTVTRDGFVIMASE
                     TGVLDIPPQDIEHKGRLQPGRMLLVDTERGRIVDDEEIKSEAGRRRPYRAWLNEQQVT
                     LTSLPRSMQPDTGEFPDLRRHQQLFGYTLEDLRIVLMPMATRGLEAIGSMGTDTPLAV
                     LSSRPQLLYNYFKQLFAQVTNPPLDAIREELVTSLHLYVGPEQNLFEETPEHAHRLKL
                     DQPILNNEELNKIRELRVGNLRAVTLPILYPVAEGGVGLRRAMDELCAAASRAIRSGA
                     TMLVLSDRGATRQKAPIPALLATAGVHHHLIREGQRTRCGLIVESGEPREVHHFAMLI
                     GYGAGGINPYLAYETLGAIVRDGWIPDIKDTLKAHTYYVKAINKGLLKVMSKMGISTL
                     QSYCGAQIFEAVGLGPEIVDRYFTATPSRVGGVGLDEIAAEVKMRHQRAFPAQDVPAL
                     LELDVGGQYQWRRGGEYHLFNPLTIAKLQQATRTGDAKVYAEYAKLINEQHTQLCTLR
                     GLLEFKSDGPPLPLEEVEPWTSIARRFKTGAMSYGSISQEAHETLAIAMNRIGGKSNS
                     GEGGEDPERFLPDPVGHWRISAIKQVASGRFGVTSNYLLSAKELQIKMAQGAKPGEGG
                     QLPAEKVYPWIAKTRHSTPYVQLISPPPHHDIYSIEDLAQLIHDLKNANTQARISVKL
                     VSEVGVGTVAAGVAKGKADVVLISGWDGGTGASPETALKHSGLPWELGLAETHQTLVL
                     NDLRSRIVVECDGKLMTGRDVAIACLLGAEEFGFSTAPLVSLGCVMMRVCHLNTCPVG
                     IATQDPDLRRKFAGKPDYAVHFFRFIAEELRQIMARLGFRTVDEMVGRVDRLEARRAV
                     EHWKARGMDLSKILFKPDMPPHVGTHRTRAQDHGIEKALDHRIIELAAPALERAEPVE
                     IDLPIRNVHRTVGTMLSSQISRYYGEEGLPDNTITLRCKGSAGQSFCAFGARGLTVTV
                     EGDANDYFCKGLSGARVAIFPPREATFVPEKNILIGNVALYGATAGDVYIRGLAGERF
                     CVRNSGARAVVEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGGIAYVLDEAGDFGRY
                     RCNPEMVELEEARDPADEAELRELVEGHWKQTGSTVARRLLDDWESALPRFVKVMPVD
                     YKRALGLVGRPQVIVPKTEPVYVSDTDVLERL"
     gene            13678..15141
                     /locus_tag="PK634_07290"
     CDS             13678..15141
                     /locus_tag="PK634_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOW97578.1"
                     /translation="MGKPTGFKEYTRELPATRPVEARVGDYRELVEPFPTDKLRQQAA
                     RCMDCGVPFCHSGCPLGNIIPDWNDLVYRNRWREALDRLHATNNFPEFTGRLCPAPCE
                     ESCVLGINEPAVTIELIEKTIIERGFENGWVVPQPPAARTGKTVAVVGSGPAGLACAQ
                     QLNRAGHAVTVFERADRIGGLLRYGIPDFKLEKGIIDRRLKLMEAEGVVFRPGVNVGA
                     DLPVEDLKNFDAVVLCGGATKPRDLNAPGRELEGIHFAMEFLGPQNRLVAGDRPEHDG
                     FRAISARGKRVIVIGGGDTGSDCIGTSLRHGAKSVTSFELMPMPPKDRPAGQPWPYYP
                     MRLRTSSSHQEGGRRFWAISTKRFVGAGGRLRSLITVNLEFVKKDGRLQMVEVPGSEH
                     EWPTELVLLALGFLGPEPDGVVARLGVDLDERGNVKTEHYATHVKGVFSAGDMRRGQS
                     LIVWAISEGREAARAVDEYLMGFSLLPAKGGPDLPRV"
     gene            complement(15142..15882)
                     /locus_tag="PK634_07295"
     CDS             complement(15142..15882)
                     /locus_tag="PK634_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOW97579.1"
                     /translation="MRFLLYNLRYCAGTGATFHFPVPGRGYLKRTSRTLRQVTHFIRS
                     QDPDIIGLVEVDSGSYRSRRLNQAEELARELGHFHVYQSKYPGDSRLHYLPLAGSQGN
                     ALLTRERIHAQRFHYFERGVKRLVIELELKNLAIFLVHLALRRRTREAQLEALVPLVR
                     AAKKPCIVAGDFNTFGGREEIQLFLRATGLRNAAGRAVLSHPSWAPKRQLDYILHGPE
                     IAVTRFAVPAVTLSDHLPLICDFKVAAS"
     gene            16011..16814
                     /locus_tag="PK634_07300"
     CDS             16011..16814
                     /locus_tag="PK634_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97580.1"
                     /translation="MNHGFDILRYRAALNQYREGFLMTYRAEIESFDAADKLFPRWLS
                     MAPPLPAAKGRDPITPAPFLLLMQRQARNAFEALTECQSARAWAALRPFVEAPLILGK
                     WLDDPQNAVVWLSRNSGKSARKDYQDVYSGDALKPQNLQGGDAIRLVLARLGDDFLQT
                     QARFYTRPVTFAPGGPRGAPDGADDPADLRAHLYAALHLLWFVLRSTGRMLAPAAGNR
                     PELKVDLERVEREFAAPVMALARENETHKNVLTQLGLWPHELLQAPRHG"
     gene            16898..19933
                     /locus_tag="PK634_07305"
     CDS             16898..19933
                     /locus_tag="PK634_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97581.1"
                     /translation="MLARRGHLRRILLLALFPAALRAATHYVSPSGLHQSPFTNWEGA
                     ATTIQAALSSVFFVTGNTVLVAAGTYTGPGNNNLDFGGRQITLQSVDGPSNTIIACTT
                     GQRGLRLEGGSVNSHVIGFTIQGATVPGAVWISSRSDPVFEDCWILNNHTLPVESRHS
                     VSSEYNDDGQLTLETTVDVDIYSGSGGGLVIQDAAPVFRRCVIGGNRAGESGGGALMV
                     SNSAPLFEDCLFLQNEAFSSSSMVQRAYAYDEFGYPVQLVETHSEAGAGEGGGVALLN
                     STARFSHCIISDNYAAQAGGGIWAAGGLVILATNRVERNRTMTNPASHSVTQTIAYDP
                     FGNPVYSQTDTGPGMYEGRGGGLALSNAAARLCGTGFFNNQAAESGGGIWLSYGSLTA
                     DTSRIEGNVSGTNSISTTFIQVIAYDEFGHVVYSSELLWASNVVAGGTGGGMAARESS
                     IHLRGSSLSSNQAKRAGGGLYAESSVIELQDVRLEDNRGSLCGAGLYMASNSSARVSD
                     SVFMQNQCSATSDRTNVTIIAYDEYGSVVYEGQEIATAEGRSFGGAIFLHAGNLLITN
                     SLFSGNATPSRGGGLFALSATAHVDSCRFYANKAQSTRFEKTVKVFSGSLVRCTTNTT
                     WWGAGGALGAESSVVAIVGCTIASNEAGDFAGGMCFQSGSTVTVENCALYDNLAGFAH
                     WSTTRLNEASGQYETVEYAVITGRGAACYVEASALTLRHVTVNANNATARMDTVCAAA
                     NASLRAVNTIFGDHSLGIETDSVAILSFSRVPPGWPGDGNLTNDARVVQDWRLAADSP
                     CIDAATNIPALAADIEGENRWDDPAHPNAGSFADMGADEFVDIDGDGMADAWEGSCVG
                     SLLTLGDGRDYDRDDAGDRDEYEHGLNPAAASSDGDPMTDGWELERGTDPLADDGQAD
                     PDNDTLVNADEFVADTDPLDGLDYLHLSQLAGGGVSNNVILSWWSSARRTYRVFTSSN
                     ILDWVEADVQPGTGTLMTYEDDRPPTRRNLLRVSAEIPP"
     gene            20247..21092
                     /locus_tag="PK634_07310"
     CDS             20247..21092
                     /locus_tag="PK634_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOW97582.1"
                     /translation="MRITGLVLTTLWLANLPAGGQEPSLTAVSLAPSLTELVFALGLG
                     DRLVGRSSACDYPPEALALPVLGDFGRPNQEALLAAHPDLVLATDLEKPGLRGYLEGQ
                     GIKVLVLSCENWTGLLDTGRAVARELGDAEAGIRWAQDMEVRRTAIEQRANTFWAGRE
                     RPSVYIEIWSDPPMTAGKESFANDLVTLAGGRNIGAALDRSYANASSEWILAQDPDVI
                     VLAYMTTNAAAERLAQRPGWDRLRAVREDHVCAGINPDWLLRPGPRMLDGAEALSEWL
                     MKTTD"
BASE COUNT         3578 a         7163 c         7165 g         3281 t
ORIGIN      
        1 gagggcggct ggacgcggct cctgcccggc ggcgagagcc ttccgatcta cgacgcggcc
       61 atgcgctatg ccggggcggg cacgccgctg gtcgtgctgg ccggcaagat gtacggcgcc
      121 ggctcgtcgc gcgactgggc ggccaagggc acgcggatgc tcggcgtgcg cgccgtgatc
      181 gccgagagct tcgagcgcat ccaccgcagc aacctggtcg agatgggcgt gctgcccctg
      241 gaattcaccg gcggccagaa cgcggagaag ctcgggctga ccgggcgcga ggagatcgcc
      301 atcgcgggca tcgccggcgg gctggcgccg ggcaagctgc tcgacgtcga cgccggcggc
      361 gcctccttcc gggtccagtg ccggatcgac tccgcgatcg aggtcgagta ttaccggcag
      421 ggcggcatcc tgccgtacgt gctgcggcag gcgatgggga cccattcgtc cggttcataa
      481 tcgtcctcgt cctcgtaatc gaaggaagag agcgcccgta agaatacgag gacgaagggg
      541 aacagatggg acaaagaggt taacatggct cacacgtaca gccccgaggc cgagaaactc
      601 ctggaccagg aaatcaaggt cctggaccag ggcttcgtcc ggctggtgga ttacatgggc
      661 agcgacgcgc ggatcgtgca gaccgcccgc gtgtcgtacg gggccggcac gaagaccgtc
      721 cgcgaagacg ccggcctgat cgactacctc cttcgccacg agcacacctc ccccttcgag
      781 cacgtggtga tcgagctgca ctgcaagctg ccgatcttcg tcgcgcgcca gtggatccgt
      841 caccggacgg cccgcctgaa cgagatctcc gcccgctaca gcgtcatggc cgacgagttc
      901 tacctgcccg acgaggcgca catcaacgcc cagagcgccg acaacaagca gggccgctcg
      961 gacgcggaag ttccgccgga gctccgccgc aaagtgctgg agctgctgca gcgcgaccag
     1021 ggcatggcct atgcctccta cgaggagatg atcaaggacg gcatcgcgcg cgagctggcg
     1081 cggatcaacc tgccgctgtc gctctacacg cagtggtact ggcagatgga cctccacaac
     1141 ctgttccggt tcctggagtt gcggatggac tcgcacgcgc agtgggagat ccgccagtac
     1201 gccgccgcga tcgcgcagat cgcgcgcgcc gtcgccccgg tggcgttcga cgccttcgag
     1261 cggcaccgcc tgaagggccg tcacttctcg gccgacgaac tcgatgccct gcggagaatg
     1321 ctcaaggggg aggaaaaccc gctgaccgga tcccggcggc gcgagtttga gcggaagctg
     1381 accgcgacct gaacgcggga ggccgtcagg aacccggcgg ccggggcgcg ggaacttgcg
     1441 ccatgacggc tctcgcggac ggcggcccct cggccaggta atgctcgtga agcgccgtca
     1501 cgcgctggac gtattcgcgg gtaaggacgg gccagcggtc aggatgaaag ccgcctttcg
     1561 cgacccgggt cggccccgcg ttgtagcagg ccagcagcag ggacaactcg tcggtcttcc
     1621 agtgggactg gttgcggccg agaaaatcct gcaggtgggc caggtaggcc cggccaacga
     1681 tgcgattcaa ctccggctcg aaggctcggt cgaaggacac cggcttgccg tacagcctgc
     1741 gcgtcacgtc gcgccaggtg ccccgcttga tctgcatcag gccgcgttcg ccggccttgc
     1801 ccaccttgcg ggggtcgccg ccggattcca cctggatcac cgcctccacc aggcaatcct
     1861 ccggaaccca gaccggcgac agttcggcgg cctccaagct ccaggacagg atctggtgat
     1921 ccgcaagctc ggcgtccgaa gagcgcgcgc tccgccaccc gcccagcgcc agttgggcca
     1981 gcagcaaagc ggagccgaac accagcagcg ccgcccggat cgccggcgcg tggcgataca
     2041 gcttgcctat gggctgtttc cagttcatcc gtcagctttt ttgcccctca ccggggccga
     2101 acaaccgaaa tccttgagaa actcgaaacg actatcggaa aaaaatgagc cggcagtgta
     2161 ccccaggaag aaacggatgc aacataaact ttatactatt ttgtcaatat atatatgatg
     2221 ttaaaacgat ttattaaaca ggaataatga tgaaatacgg tgggcagtat atgcccctgc
     2281 gacccctatg tgccggtttc tcacccgttt ccaggctcgg aagcccgcag cgggtcgcgg
     2341 atatacgctt tctggcaggc cgggcaaagg tcgaaactga aatccacgta gacgtcccgc
     2401 atgagttcct cctcggtgag gtcccggcac tcctcgatta tgcgccggat ttccgccgaa
     2461 tagtcgcggc ccaggtcgtc gaaggtgatt tccagcgggt cgtaggccgc atagacctgg
     2521 atgcgcgcga tgtagcgaag cccccccgtt tcgagcggtt gcccgaagcg atcgcagata
     2581 cggctgatca tgtccgcctc ccttacgggc cacttgagcc ttgtccggaa caggcctttt
     2641 ctctatggtg gcaggccccg gggcgaaggg cgagcaaaag agggaaagga tcacggagat
     2701 gaccacggcc agcgatggga aatgggtgtg cacctgctgc ggttatgtct acgacccggc
     2761 cacgggcgac gcggcgctcg gcctgccggc cggcgtgccg ttcgagcagt tgcccgagaa
     2821 ctggaagtgc ccgatatgct acgcggccaa ggacgcgttc gatcccctgt gaggcgcgcc
     2881 gatttgactc ggcgcgaccg ggctcgcctt cctgctctgc ccgccgcggc tcgtgaaacg
     2941 gaggttccac gtccccgcct ccgcgctgat ggaagacgaa aaaaaggccc cgacgccggg
     3001 cgtcggggcc gatgcggact ggcgctggac cgggtcaacc ggccgggggt gccaccggcg
     3061 tcttcggggc gggcgccttc ttcgccgccg ccttcttcac gcgcgccttc ttcgccgcgg
     3121 gtttcgcggc ggccggcttg ccggcccggg acagcgcgta gcggccgcgc cggacgctgc
     3181 ggaaccgctt gtccttgacg agcgtctggt tgacgatcag cttgaagttc ttggccttgg
     3241 acttgtatcc cgtcttggcc acggcttccg ccgcctcggc gaccttcatg gacttgatcc
     3301 cggtcagcac tttgaccagg acgtcgccca gcgactgggc gttcttgacc cggcgggcct
     3361 ttccggccgg acggccgcgc ttcgcggggc gaccgcgctt ggcgggacgg ccgcgcttcg
     3421 ccggacgccc gcggcggggc tgggctttcc cggtcccttt gatccgtgaa acgacgacgc
     3481 cgagatcggc gatcgcctct tccaccttcg ccagggccgc ggccagcttc ttccgcttct
     3541 tctccaggcg cggcagcgcg ccctgccgtc gcttcaattc gccctgcaac tgggccacac
     3601 tcaactgctt caaggccact cgtgccattt cccttatctc cttctcatcc aattgaggat
     3661 gttaggtgcg caataaaccc cgcggcgcct aacttgtcaa actaaattag attcgcgcca
     3721 tacactaatt taggttccgt tccgtattat ggtataagaa agcgcatggc ggcggaacat
     3781 ggcatcctgg acgcgcaggc ggcggcctgg ctggacgagt accggcaagg ccgggtcgag
     3841 gccttgggcc gcgtggtgga gcagtatcgc cgcccgctct tcgggttcat tctcaacatg
     3901 acggaggggc gcgaggatgc ggaggaggta ttccaggaga cctggcgccg ggccctgcgc
     3961 cacgcggacc gactggacgg ccggcgcctg ttgagctggc tgttccgggt ggcccacaac
     4021 ctgatcgtgg accgcgtccg cgcgaaaaag ccggtcgaat cgctgaacag cgagccccgg
     4081 gccgaggagc cgggacgcgc cccgctcgcg gaccgcctcc cggcgccggg cccgacgccg
     4141 gccggcgaga gcgcggggcg ggatctcgat cgccgcatcc gacaggccat cgaccggctg
     4201 ccgcccgagc agcgggaggt cttcctgatg cgcatcgagg gcgacgtgcc gttccgcgag
     4261 atcgcccggc tccaggaggt ctcgatcaat acggcgctgg cgcgaatgca gtacgcgctg
     4321 aaaaagttga gggaagattt gaaggacgaa tatgccgcgt gggtggagga atgatcatga
     4381 actgtgaaac gaccaggaac cgcattctgc tggaccagtc gcgggagctt tcccgggtcg
     4441 gccgctggct gctcgccggg cacctggccc gctgccccga atgccgcgcg taccgcgccg
     4501 ggctgaccgg actggctggg gccgtccgcc aggtgcgctg ggacgagtca ttcgacgccg
     4561 ggctggccgg gcgggtcgcg gccggcgcgg ccgtccccgc cccccgcgcg aaacgcgggc
     4621 gagaacgcgc gcccgtcttc ctggcgcgcc cggcgctggc ctacggggcg ctcagcgtcc
     4681 tgctcgccct ggcgttcgtg ctgatcctgc ggcctttcca gcggccgccg ccactcgccc
     4741 ggaccgcgcc ggactccgcg ctcgcgtggg acgccgacgt ggacgaccgc ctggccacgc
     4801 tcgacgtcct ggtcaaccag gacgacgcgg actggaccga aacgtccggc gcggccgcgg
     4861 acagcggcga cgtggattcg atcgcccggc agttgctgga gctggaagga gagcagatat
     4921 gaaaagcatc caccgacgat ttctgacggc gggcatcatc gcgggcctgg ctttcgcgat
     4981 tcgcgcagca gcccaggact tcgacgaagc ctcgccgccg cctcccgagg cgccggacct
     5041 ggcggagccg ccggggccgc cgccgggcga agccgggccc gtggaccgct ggatggagcg
     5101 gctggagaaa cgcaacccgg acgaatacct tcgctaccgc gaaatgcggg agaaggatcc
     5161 cgaggggttc cgccgggaac tcgcggggcg gctgggccgc gagcggctgc agacggtcat
     5221 gaacgcccat ccccggctgc gggtgttcct cgacagcctt ccggaatcgg aacgcatgga
     5281 cgtgctccag gcgctgggcc gcgccctccg gcccggcggc ccgccgcacg aaccaaacgc
     5341 cggcgaactg cggccgctcc acgaagaggt ggcccggctg gcccgggagt accggcagag
     5401 cacggaccag gcgcagcggg agcaacggcg ggccgacctg aaactcaagc tgggcgagct
     5461 gttcgacgcc cgggaacaag gacgggcgga tcacatcgcg cgcatccagg ccgaggtggc
     5521 caagctgcag caggcgctgg attcccgccg ggcgcagcgc gacgagatcg tcgaacgacg
     5581 gcttcaggag ctgaccgcgg acgaaagctc gaagtggtag ggcgagaccg gccgcgcccg
     5641 acgcccgggg ttcagcggac ccgcggggcg tcgccccaga gcttctccag cgcgtagtag
     5701 gaccgggcgt cggcggagaa gaggtggatc accgcatcga ggtaatcggc gaccacccag
     5761 ccgctctccg gcgcgcccgc gcgccggaag catcgcgcgc cgccgcggga gagggcgcgt
     5821 tccagctcat ccgccatggc cttgaggtgg ggcgcgttgc ggcccgtgac gaccaggtag
     5881 taatcggtaa ccccggagag tccgcgcatg tccagcagga cggggtcttc ccccttccgg
     5941 tcggacagga cctcgcgggc cagcttggct aattggagcg gttcgatctt gcgttctcct
     6001 taccggtaca gtttcttgct tcgaatatac ccggcgacgg ccggcgcgac aagatagcgg
     6061 aggctcaatc cggcggccgc gcgcgcccgg atctcggtgg aggaaatctc catcacgtgc
     6121 ccgcggaaga cggtccgcgc caggcgccgg ggccagggct ccggtaggcg caggcggcgc
     6181 gcggtcatcc gggccaggga atagcccggc cgctccagcg cgatgaacac gcagcgccgc
     6241 aacagctcgc cgacctcccg ccaggcgtgc agttcgaaca gcgaatccga cccgatgatg
     6301 aaatgcagcc gcgcccgcgg gtggttgcgc tgcaacgcct tgatcgtgtc gatggtgtac
     6361 gagatgccgc cccgccggat ctccaggtcg gagaccgtga accggggatc gccgcgaacg
     6421 gcccggcgca gcatcgccag ccggtcggcg tccggcgcga gatcccgcgc ggccttgtgc
     6481 ggcgggcggg cgcagggcat gaagatcacg cgatccaggc ggaagcgctc gagcgcgtcc
     6541 tgggccatca tgagatgccc gaggtgcacc gggttgaacg tcccgccgag tatgccgagg
     6601 cggtcgctca caggtgcagg ccgatgctgc tcaatttcca ctggggatgc tcgtagatga
     6661 acgacagctc gaaccggacc tggtccgggg tcgtggcata gtagccgcgg aggttcaacg
     6721 cgccgttctc gtccaccgcg ggcttctggc tgaagaccgg cgtctggctc ttcagcgggg
     6781 ccaagtcgat ctgcttctcg gcgaattcct tgaaggcttc ccggagttgc tcctcggtga
     6841 cctggctttg ccacaactgc gagatgtgcg cgcggaagcc gctgaagtcg tttttgttga
     6901 tcgcctccgc caggaggccg atggcttccg aggtcatgcg ctgaagttcg gcgtccggcg
     6961 ggatctcgac ggtcgaggcg ccgccctggg caagccccgc ggcggccttg cgcaggttga
     7021 ggatcttcca cgcgccgcct tccttcacga ggtccagttc caccgggatc tggccgccgt
     7081 cgcgggtcgt cacggtcccc tcgagccggc cccggttgtt ctcgatggcg cgggtcggcc
     7141 acgaagcgct cgcgtactgc ccgagcgcgc tgtcccggag gaacgccgaa aactcctcct
     7201 cgcccgtcgc cgcgcggaac tcggcggccg tggcgagata ggcctcgccg actttcccct
     7261 cgcggaccag ggagaaaaat tcgtccgcgg ccttcggcag gccgctcgtc gcaaagaaca
     7321 gcagggcgat caggatggcc accaccacca cgatcccgag cagaatcgtc cccatttttt
     7381 tcatcgcggc ctcctccgtt cggccagctt tcattatccc ccgcccggcc cgcgagtcaa
     7441 cgcccgcttc cggcgcccgc ccgtttgacg aaccggcccg cgcaatgcta tagacagcca
     7501 tccagcggaa ttacaatttt gtatgaaacg cccgcaacgg ctatacaaaa aagtagcata
     7561 taaacggtct gtaaattgaa attgctgctt ttccgcggga aaccgtcggg cgacgcgaag
     7621 atggaatggt tctagcttga agaaaaagga aggggcagaa gcatgaacaa tcggaacggg
     7681 aagttggatc tggcggagcc acgggaggct tgcgggctgt tcggcgtctt cggcgtgccg
     7741 gagacggccc gggcgattca tgccggcctc tttgcgctgc agcaccgggg ccaggaaggc
     7801 gccgggatcg tcgtcagcaa cggggagaag atccgctcga tcaagggcat gggcctggtg
     7861 gcggaggtct tcgcgacccg ctccctcgcc gagctcgagg ggcacctcgg catcgggcac
     7921 gtccgctatt ccaccaccgg ctcgtcccgc ccgcagaacg tgcagccgat cgtcttcgag
     7981 tgcgtggacg gcctgtgggc gctggcgcac aacggcaacc tgaccaattc ccaggcgatg
     8041 cggcgctcgt accaggaggc cggcgcgatc ttccagacga gcacggacag cgagatcctg
     8101 atgcacctga tcgcggaccc ggcctaccgc ggccggccgc gccgcgtgga acgcgcgctc
     8161 gcggagctgc agggcgcctt ctccttcctg ctgatgaccc gcgactcggt cctggcggcg
     8221 cgggaccgct acgggttccg cccgctgtcc gtcggccgcc tgggcggggg gtatgtcttc
     8281 gccagcgaaa cctgcgcgct ggagcaggtc ggcgccacct tcgagcgcga cgtgctgccc
     8341 ggcgagctgg tggtcgtcga cgcgaagggc atccggggca gcacgttctg cgaaccggtc
     8401 agcgggccgc tgggccagtg catcttcgag cacgtctact tcgcgcggcc ggacagcacg
     8461 atcttcggcc agagcgtcca cgaaacgcgg gtcgcgctcg gccgccagct cgcgcgcgag
     8521 catcccgtcg aggccaacct cgtgatgccg gtcccggaca gcggcagctc ggcggcgctc
     8581 ggctactcgc tggagagcgg cctgccgatg gattacggtt tcatccgcaa ccactacatc
     8641 gggcggacgt tcatcatgcc ggagtcggac cagcggtcgg gccaggtgga cctgaagctg
     8701 gccgtcgtgc ccggcgtggt gcgggacaag cgggtcgtgg tcgtggacga ctcgatcgtc
     8761 cgcggcacga cgtcgcgccg ccggatcgcg gccctgcgca aggccggcgc gcgggaaatc
     8821 cacctgcgca tctcgtgccc gcctatccgg cacccctgct atttcggcat cgacttcccg
     8881 tcgcgcaagg acctggcggc ggcccagcac ccgctggagg atatccgtcg gttcatcggg
     8941 gcggacagcc tggccttcct ccggctggag ggcctgctgg cggcgttcga gcagccgggc
     9001 gactactgca cgggctgctt ctccgggaag tacccggcgc ccgttccgga acaacactcc
     9061 aaaaccgcgc tggaaaatgc gcaggcggcg cccgcatgaa tccccttccc cccgcggccc
     9121 agggcctcta tcacccgcgc cacgagcacg acgcctgcgg cgtcggcttc gtctgccaca
     9181 tgcgcggcga gaaatcgcac gacgtgatcc acaaggccct gcgcatcctc gtcaacctga
     9241 cccaccgcgg cgcgtgcggc tgcgacgaga agaccggcga cggcgcgggc atcctcatgc
     9301 agttcccgca cgccttcttc gcgggccgcg cgcccgaggc ggggctgaag ctgccgggcg
     9361 agcgggagta cggcgccggg ctggtcttcc tgccccgcga cgcgcgcgag cgcggacact
     9421 gcgtcaggct cttcggggac gcgatccggg ccgagggcca ggtcctgctc ggctggcgga
     9481 acgtgccggt gcacaacgag gggatcggcg acctggcgcg gcaggtcgag ccgttcatcg
     9541 cccaggtctt catcggccgc ggacccggcc tcgacgacga ggctcacttc gagcgcaagc
     9601 tgttcgtcat ccgcaaggtc atggagcgcg cggtccgcga gtccgatctg cgcgagaaga
     9661 agtacttcta catcccgagc ctttccagcc agacgttcgt gtacaagggc ctgctgctcg
     9721 ccgaccagat cgagccgttc ttcccggacc tgaccgacac ggccatggtc agcggcctgg
     9781 cgctggttca ccagcgttac agcaccaaca cgttcccgac gtgggacctg gcccagccgt
     9841 tccggttcct gtgccacaac ggcgagatca acaccctgcg cggcaacatc aactggatga
     9901 acacccggca gtatctcttc cagtcgacgc ggttcggcga cgacatggcc aagctcttcc
     9961 ccatcgccac gccgggcgcg agcgactcgg cgatcctgga taacgccgtc gagcttctgt
    10021 accacaccgg ccgccccctg ccccaggcgg tgatgatgct catccccgag gcgtggcaga
    10081 accaccgcac gatgccggac gagaagaagg ccttctacga gtaccacgcc aacctgatgg
    10141 agccgtggga cggcccggcc tccatcccgt tcaccgacgg ccggtgcatg ggcgcggtgc
    10201 tggaccgcaa cgggctgcgc ccgtcccgct acacggtgac gcgcgacggg ttcgtgatca
    10261 tggcctccga gaccggcgtc ctggacatcc cgccgcagga catcgagcac aaggggcggc
    10321 tccaaccggg ccgcatgctg ctggtggaca ccgagcgcgg gcggatcgtg gatgacgagg
    10381 agatcaagag cgaggccggc cggcgccgcc cctaccgcgc gtggctgaac gagcagcagg
    10441 tgacgcttac atccctgccg cggtccatgc agccggacac gggcgagttc ccggacttgc
    10501 ggcggcacca gcagctgttc ggctacacgc tcgaggatct gcggatcgtc ctgatgccga
    10561 tggcgacccg cgggctggag gcgatcggct cgatgggcac cgacacgccg ctggccgtgc
    10621 tctcgtcgcg gccgcagctg ctctacaact acttcaagca attgttcgcc caggtgacca
    10681 atcccccgct cgacgcgatc cgcgaggagc tggtgacgtc gctccacctg tacgtcggtc
    10741 ccgaacagaa cctcttcgag gagacgccgg agcacgccca ccggctcaag ctggaccagc
    10801 cgatcctgaa caacgaagag ctgaacaaga tccgcgagtt gcgcgtcgga aacctgcgcg
    10861 cggtcacgct cccgatcctc taccccgtcg ccgagggcgg cgtggggctc cgccgggcga
    10921 tggacgagct gtgcgcggcc gcgtcgcggg ccatccgctc cggcgcgacg atgctggtgc
    10981 tctccgaccg cggggcgacg cggcaaaagg cgccgatccc ggcgctgctg gcgacggccg
    11041 gcgtgcacca ccacctgatc cgcgagggcc agcgcacgcg ctgcgggctg atcgtcgaga
    11101 gcggcgaacc gcgcgaggtg caccacttcg ccatgctcat cggctacggg gccggcggga
    11161 tcaaccccta cctggcctac gagacgctcg gggcgatcgt gcgcgacggc tggatccccg
    11221 acatcaagga cacgctcaag gcgcacacct actacgtcaa ggcgatcaac aagggcctgc
    11281 tgaaggtcat gtccaagatg ggcatctcga ccctgcagag ctactgcggc gcgcagatct
    11341 tcgaggcggt cgggctgggg cccgagatcg tggaccgcta cttcacggcc acgccgtcgc
    11401 gcgtcggcgg cgtggggctc gacgagatcg ccgccgaggt gaagatgcgc caccagcggg
    11461 ccttcccggc ccaggacgtg ccggcgctcc tggaactcga cgtcggcggc cagtaccagt
    11521 ggcggcgcgg gggcgagtac cacctgttca acccgctgac catcgcgaag ctgcagcagg
    11581 ccacccgcac gggcgacgcc aaggtctacg ccgagtacgc gaaactgatc aacgagcagc
    11641 acacgcagct ctgcaccctg cgcgggctgc tggagttcaa gtcggacggc ccgccgctgc
    11701 cgctggagga ggtcgagccg tggacgtcca tcgcgcggcg gttcaagacg ggcgcgatgt
    11761 cctacggctc gatcagccag gaggcgcacg agacgctcgc gatcgccatg aaccgcatcg
    11821 gcggcaagag caacagcggc gagggcggcg aggaccccga gcgcttcctg cccgacccgg
    11881 tcggccactg gcggatcagc gcgatcaagc aggtcgcctc cggccgcttc ggcgtgacga
    11941 gcaattacct gttgagcgcc aaggagctgc agatcaagat ggcccagggc gccaagcccg
    12001 gcgagggcgg ccagctcccc gcggagaagg tctatccctg gatcgcgaag acgcggcact
    12061 cgacgccgta cgtgcagctc atctcgccgc caccgcacca tgacatctac tcgatcgagg
    12121 acctggcgca gctgatccac gacctgaaga acgccaacac ccaggcacgg attagcgtga
    12181 agctggtttc cgaggtcggc gtcggcacgg tggccgcggg cgtggccaag ggcaaggcgg
    12241 acgtggtgct gatcagcggc tgggacggcg gcaccggcgc ctcgcccgag accgcgctga
    12301 agcactccgg cctgccgtgg gagctgggcc tggcggagac gcaccagacg ctggtgctca
    12361 acgacctgcg cagccggatc gtcgtcgaat gcgacggcaa gctgatgacc gggcgcgacg
    12421 tcgccatcgc ctgcctgctg ggcgcggagg agttcggctt ctcgaccgcc ccgctggtct
    12481 cgctgggctg cgtgatgatg cgggtctgcc acctcaacac ctgccccgtc ggcatcgcga
    12541 cgcaggaccc ggacctgcgc cggaagttcg cgggcaagcc ggattacgcc gtccacttct
    12601 tccggttcat cgccgaggag ctgcggcaga tcatggcccg gctgggcttc cgcacggtgg
    12661 acgagatggt cgggcgcgtg gaccggctcg aggcgcgccg cgcggtcgag cactggaagg
    12721 cccgggggat ggacctctct aagatcctct tcaagcccga catgccgccc cacgtcggca
    12781 cccaccggac ccgggcgcag gaccacggca tcgagaaggc cctggatcat cggatcatcg
    12841 agctggccgc gccggcgctc gagcgcgcgg agccggtcga gatcgacctg ccgatccgga
    12901 acgtccaccg gacggtcggc acgatgttga gcagccagat ctcgcgctac tacggcgagg
    12961 aaggcctgcc ggacaacacg atcacgctgc gctgcaaggg ctccgcgggc cagagcttct
    13021 gcgcgttcgg cgcgcgcggc ctgaccgtca cggtcgaggg cgacgcgaac gattacttct
    13081 gcaagggcct gtccggcgcc cgcgtggcga tcttcccgcc gcgcgaggcg acgttcgtcc
    13141 cggagaagaa catcctgatc ggcaacgtgg ccctctacgg cgccacggcc ggcgacgtgt
    13201 acatccgggg cctcgcgggc gagcgcttct gcgtgcgcaa cagcggcgcg cgcgcggtcg
    13261 tcgagggcgt cggcgaccac ggctgcgagt acatgaccgg cggccgcgcg gtgatcctcg
    13321 ggccgaccgg ccggaacttc gcggccggca tgagcggcgg catcgcctac gtcctggacg
    13381 aggccggcga tttcggacgc taccggtgca acccggaaat ggtcgagctc gaggaggccc
    13441 gcgacccggc ggacgaggcc gagctgcggg agctggtgga gggccactgg aagcagaccg
    13501 gcagcacggt cgcgcggcgg ctcctggacg actgggagtc cgccctgccc cgcttcgtca
    13561 aggtcatgcc ggtggactac aagcgcgccc tcggcctcgt cgggcggccg caggtgatcg
    13621 tgccgaagac cgagccggtg tacgtcagcg acaccgacgt gctggaaagg ttgtgacatg
    13681 ggcaaaccca caggcttcaa ggaatacacg cgcgagctgc cggcgacgcg gccggtcgag
    13741 gcgcgggtcg gcgactaccg cgagctggtc gagccgttcc cgacggacaa gctgcgccag
    13801 caggcggcgc gctgcatgga ctgcggcgtg ccgttctgcc acagcggctg cccgctcggc
    13861 aacatcatcc cggactggaa cgacctggtc taccgcaacc gctggcgcga ggccttggac
    13921 cgcctgcacg cgacgaacaa cttccccgag ttcaccggcc ggctgtgccc cgccccgtgc
    13981 gaggagtcct gcgtgctggg catcaacgag cccgcggtga ccatcgagct gatcgagaag
    14041 acaatcatcg agcgcgggtt cgaaaacggc tgggtcgtcc cgcaaccgcc ggccgcgcgg
    14101 acgggcaaga cggtggccgt cgtcggctcc gggccggcgg gccttgcctg cgcccagcag
    14161 ctcaaccgcg cgggccacgc cgtgaccgtc ttcgagcgcg cggaccggat cggcggcctg
    14221 ctgcgctacg ggatccctga cttcaagctg gagaaaggga tcatcgaccg gcgcctgaag
    14281 ctgatggagg ccgagggcgt cgtgttccgg ccgggcgtga acgtgggcgc ggacctgccc
    14341 gtcgaggact tgaagaactt cgacgccgtg gtcctgtgcg gcggcgcgac gaagccccgg
    14401 gacctgaacg cgccgggccg cgaactggag gggatccatt tcgccatgga gttcctcggg
    14461 ccgcagaacc ggctcgttgc cggcgaccgg ccggagcacg acgggttccg cgcgatctcc
    14521 gcgcgcggca agcgggtcat cgtcatcggc ggcggcgaca cgggcagcga ctgcatcggc
    14581 acgagcctgc ggcacggcgc gaagtccgtc accagcttcg agctgatgcc gatgccgccg
    14641 aaggacaggc cggccggcca gccgtggccc tactacccga tgcgcctgcg cacgagctcg
    14701 tcgcaccagg agggcgggcg ccggttctgg gccatcagca ccaagcgctt cgtcggcgcg
    14761 gggggccgcc tccgcagcct gatcacggtc aacctcgagt tcgtgaagaa ggacggccgc
    14821 ctgcaaatgg tcgaggtgcc gggctcggag cacgagtggc cgaccgagct ggtccttctg
    14881 gccttggggt tcctcgggcc ggaaccggac ggcgtcgtcg cgcggctggg cgtggacctg
    14941 gacgagcgcg gcaacgtgaa gacggagcac tacgccaccc acgtcaaggg tgtgttttcg
    15001 gccggcgaca tgcggcgcgg ccagtcgctg atcgtctggg caatctccga gggccgcgag
    15061 gccgcgcggg ccgtggacga gtacctgatg ggcttttcgt tgctgccggc caagggcggg
    15121 ccggacctgc cgcgggtatg atcagctcgc cgctaccttg aagtcgcaga tcagcggcag
    15181 gtggtcggac agcgtcacgg ccggcaccgc gaaacgcgtc accgcgatct ccgggccgtg
    15241 caggatgtag tcgagctgcc gcttcggcgc ccagctcggg tgggagagca cggcccggcc
    15301 cgcggcattc cgcagccctg tcgcgcggag gaacagctgg atctcctccc ggccgccgaa
    15361 cgtgttgaaa tccccggcga caatgcacgg ctttttcgcc gcccggacca gcgggaccag
    15421 ggcctccagc tgcgcctccc gggtgcgtcg ccgcagggcg agatgcacta ggaagatcgc
    15481 caggttcttg agttccagct cgatgaccaa ccgcttcacg ccgcgctcga agtagtggaa
    15541 gcgctgcgcg tggatccgtt cgcgcgtcag cagggcgttg ccctggctcc cggccagggg
    15601 caggtagtgc agccgggaat cgccggggta cttggactgg tacacgtgga agtggcccag
    15661 ctcgcgggcc agttcctccg cctggttcag ccggcgggag cggtaggaac cgctgtcgac
    15721 ctcgaccaag ccgatgatgt cggggtcctg ggagcggatg aagtgggtca cctgccgcaa
    15781 ggtccgcgag gtgcgtttca ggtagccgcg cccggggacg gggaaatgaa acgtcgcgcc
    15841 ggtgccggcg cagtatcgca ggttgtagag caggaatcgc acgccgctat ccttatcccg
    15901 gacgcgcccc ggatgcaacg acggagcggg cgggcggatc gcgaaatcgc tttcctgccc
    15961 cgcgcgggac gctatgatgg ggtgcaaaca accggacgga gaaccgcacc gtgaaccatg
    16021 gcttcgacat cctccgctat cgcgccgcgc tcaaccagta ccgcgaaggg ttcctgatga
    16081 cctaccgcgc ggagatcgag tcgttcgacg cggcggacaa gctgtttccc cgttggctgt
    16141 ccatggcgcc gcccctgcct gccgcgaagg gccgcgaccc gatcacgccg gcgccgtttc
    16201 tgctgctcat gcagcggcag gcgcggaacg ccttcgaggc gctcacggag tgccagtccg
    16261 cccgcgcctg ggcggcgctg cggccgttcg tcgaggcccc gctgatcctg ggcaagtggc
    16321 tggacgatcc gcagaacgcc gtggtctggc tctcccgcaa ctcgggaaaa agcgcgcgca
    16381 aggactacca ggacgtctat tctggcgatg cgctcaagcc ccagaacctg cagggcggcg
    16441 acgcgatccg actcgtcctg gcccggctcg gtgacgattt cctccagacc caagcgcgct
    16501 tctacacgcg gcccgtcacg ttcgcgcccg gcgggccccg gggcgcgccc gacggagcgg
    16561 acgatcccgc cgatctccgg gcgcacctct acgccgcgct gcacctcttg tggttcgtcc
    16621 tgcgctcgac cggccgcatg ctggccccgg ccgccgggaa ccggcccgag ttgaaagtgg
    16681 acctggagcg cgtcgagcgc gaattcgccg cgccggtcat ggccctcgcc cgggaaaacg
    16741 agacgcacaa gaacgtcctg acccagctcg gcctgtggcc gcacgagctg ctccaggccc
    16801 cgcggcacgg gtaaccggtc ccggaggcct gacgaaacga cgcgcggcac tcccctttgc
    16861 agttggcccg cgcccgcaac cgcgctatca tggcctcatg ctggcgcgca gagggcatct
    16921 tcgccggatt ttgctgttag cgctttttcc cgccgcgctc cgggccgcca cgcattatgt
    16981 ctccccctcc ggcctccatc agtcgccgtt caccaactgg gaaggcgccg ccaccaccat
    17041 ccaagcggcg ctgagcagcg tgttcttcgt cacgggcaat acagtcctgg tggcggcggg
    17101 tacgtacacc ggccccggca acaacaatct ggattttggg ggcaggcaga tcacgctcca
    17161 gtcggtcgac gggcccagca acacgatcat cgcctgcacc accggccaac gaggacttcg
    17221 tctcgagggc ggcagcgtaa actcgcatgt gatcggcttc accatccaag gcgccaccgt
    17281 tccaggagcc gtatggatct cctcccggtc cgatcccgtt ttcgaagact gctggattct
    17341 caataaccac acccttcccg tcgagtcgag gcactccgtc tcgtcggaat acaatgacga
    17401 cggccagctc accctcgaga ccacggttga tgtagacatc tactcgggat cgggaggcgg
    17461 cctcgtgatc caggatgccg cgccggtctt tcgccggtgc gtgatcggcg gcaatcgcgc
    17521 cggcgaatcc ggaggcggag ccctcatggt gtccaacagc gccccgcttt ttgaagactg
    17581 tctcttcctg cagaatgaag ccttttcctc ctcctcgatg gttcagcgcg cttatgccta
    17641 tgacgaattc ggatatccgg tgcagttggt tgaaacccat tccgaggcgg gcgcgggtga
    17701 gggaggcggc gtcgcgctcc tcaattccac ggcccgattc agccactgca tcatctccga
    17761 taattacgcc gcccaggcgg gaggcggaat ctgggccgcc ggcggattgg tcatcctggc
    17821 cacgaaccgt gttgagcgca accgcaccat gaccaacccg gcttcccatt cggttacaca
    17881 gaccatcgcc tacgatccgt tcggcaatcc ggtttatagc caaacagaca caggccccgg
    17941 gatgtacgaa ggccgcggcg gcggcctcgc gctttccaac gccgcggccc gactctgcgg
    18001 aaccggtttt tttaacaacc aggccgctga atccggcgga ggaatctggt tgtcgtacgg
    18061 ctcgctcacg gccgacacca gccggatcga aggaaatgtc tcgggcacca acagcatcag
    18121 caccacgttt attcaggtca tcgcctatga tgaattcggc catgtcgtct actcatccga
    18181 actcctttgg gcctccaatg ttgtcgcggg cggcaccggg ggcgggatgg cggcccggga
    18241 gtcctcgatc cacctgcgag ggagcagctt aagctccaat caggccaaga gggcgggcgg
    18301 cggtctctac gccgaatcgt ccgttattga actgcaggat gtccgactgg aggacaatcg
    18361 cggctcccta tgcggagcgg gcctgtacat ggcttccaat tcgtccgccc gtgtatccga
    18421 ctctgtattc atgcaaaacc aatgcagcgc gacgagcgac cgaaccaatg tgaccatcat
    18481 cgcctacgac gagtacggaa gcgtggttta cgagggccaa gagatcgcga cggctgaagg
    18541 ccgctcgttt ggaggcgcca tctttcttca cgcaggcaac ctgttgatca ccaacagtct
    18601 cttcagcggc aacgcgacgc caagccgcgg cgggggattg ttcgctttgt cggccacggc
    18661 tcacgtggac tcgtgccgtt tttacgccaa caaggcccag tccactcgat ttgaaaagac
    18721 cgtcaaagtg ttctccggtt ccctggtccg ctgcacgacc aacacgacct ggtggggagc
    18781 gggcggggcg ctgggcgcgg aatcgtcggt cgtggccatc gtcggctgca cgatcgcgag
    18841 caacgaggcg ggcgatttcg cgggcgggat gtgcttccag tccggttcga ccgtgaccgt
    18901 ggagaactgc gccttgtatg ataacctagc cggcttcgcg cattggagca cgacgcgctt
    18961 gaacgaggcc agcggtcaat acgagaccgt cgagtacgcg gtcatcaccg gccggggcgc
    19021 ggcgtgctac gtcgaggctt ccgcactgac cctgaggcac gtcactgtga acgccaacaa
    19081 cgccacggcg cgcatggaca cggtgtgcgc cgcggccaac gcctcgctgc gggcggtcaa
    19141 cacgatcttc ggcgaccact ccctgggcat cgagaccgat tccgtggcca tcctttcctt
    19201 ctcgcgcgtt ccccccgggt ggccgggcga cgggaacctc accaacgacg cccgcgtggt
    19261 ccaggactgg cggctcgcgg ccgattcgcc gtgcattgac gcggccacga atatcccggc
    19321 cctcgccgcg gacatcgagg gggagaaccg ttgggacgat cccgcgcacc cgaatgccgg
    19381 ctccttcgcc gacatgggcg cggacgagtt cgtggatatc gatggcgacg ggatggccga
    19441 tgcctgggag ggatcctgcg tcggcagcct gctgactctg ggcgacggcc gggactacga
    19501 ccgtgacgat gccggggacc gggacgaata cgagcacggc ctgaatcccg cggccgccag
    19561 ttccgacggc gacccgatga cggacggctg ggaactggag cgcgggaccg atccgctcgc
    19621 ggatgacggc caggcggacc cggataacga caccctggtc aacgcagacg agttcgtcgc
    19681 cgacaccgat cccctcgacg gattggatta tctccacctc tcccaactgg ccggcggcgg
    19741 cgtgagcaac aacgtcatcc tctcctggtg gagcagcgcc cggcggacct accgcgtttt
    19801 tacatccagc aacatcctgg actgggtcga ggcggacgtt cagccgggca cggggaccct
    19861 catgacgtac gaggacgaca ggccgccaac tcggaggaac ctgctccgcg tcagcgccga
    19921 gattccgccc tagcccgctt attgcatgag cgtagagacg ctcacgcaat aagcgcttct
    19981 gggcacgcga attgcatagg caattcgcgt gccagaagac gtgacgtatt gtcgaatcaa
    20041 ggattcgaca atacgaacgt ggcaagcccc gtttgtgccc gcaacgcggg cacatcaccg
    20101 cgtgcggcca cgttgccgcg gcctacgcgg ctcacgtggc ggccctttgg gcggataatg
    20161 cgcgccaatc gcgcattatc cgggctagca ggccgccagg taaaaggatt tgtcggcctc
    20221 tcccgagaag cctaggatgc tgctccatgc gcataaccgg gctcgttttg acaacgcttt
    20281 ggctggcgaa tttaccggcc ggaggccagg aaccgtccct caccgccgtc agcctggccc
    20341 ccagcctgac ggaactcgtc ttcgcgctcg ggctcggcga ccggctcgtc gggcgatcct
    20401 cggcctgcga ctacccgccg gaagccctgg cacttccggt gctcggcgat ttcgggcggc
    20461 cgaaccagga ggccctgctg gcagcgcatc ccgatctggt cctggccacc gacctcgaaa
    20521 agcccggcct gcgtggatac ctagagggtc aaggtatcaa agtgctggtc ctgtcgtgcg
    20581 agaactggac gggcctgctg gacactggac gcgccgtcgc ccgcgagctc ggggacgccg
    20641 aagccgggat ccgctgggcg caggacatgg aagtccggcg gacggcgatt gaacagcgcg
    20701 cgaacacttt ctgggccggg cgggaacggc ccagcgtgta catcgagatc tggagcgatc
    20761 cgccgatgac cgcggggaag gaatccttcg cgaacgacct ggtcacgctg gcgggggggc
    20821 gaaatatcgg cgcggcgctg gaccgctcgt acgcgaacgc gagttcggaa tggatcctgg
    20881 cgcaggatcc ggacgtcatc gtgctcgcct acatgaccac gaacgcggcg gcggaacggc
    20941 tggcccaacg gccgggatgg gaccggctcc gggccgtccg cgaggaccac gtttgcgccg
    21001 gcatcaaccc ggactggctc ctgcgcccgg gtccgcgtat gctcgacggc gcggaggcgc
    21061 tctcggaatg gctgatgaaa acaacggatt gaccacggat ttcgcggatg agcgcggatg
    21121 acctatccgc gaagtccgcg ctatccacgg ccccgttcct tcggatgaat aaacaaagaa
    21181 tattcat
//
LOCUS       DAOOTK010000049        19706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1897_length_19706_cov_5.440639, whole genome shotgun sequence.
ACCESSION   DAOOTK010000049 DAOOTK010000000
VERSION     DAOOTK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19706
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_19706_cov_5.440639"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..442
                     /locus_tag="PK634_07315"
     CDS             215..442
                     /locus_tag="PK634_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011105773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOW97583.1"
                     /translation="MPENIVGTQVRRLRYQQELSQALLAAKLNRLGWDISRGTLAKIE
                     AGIRCISDKEVFLLAKALKVPLNQLYSGGRA"
     gene            complement(1018..1094)
                     /locus_tag="PK634_07320"
     tRNA            complement(1018..1094)
                     /locus_tag="PK634_07320"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1058..1060),aa:Asp,seq:gtc)
     gene            complement(1164..2384)
                     /locus_tag="PK634_07325"
     CDS             complement(1164..2384)
                     /locus_tag="PK634_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOW97584.1"
                     /translation="MRIKAFQALRPPADRAARVASPPYDVVDTAEARRQAEGNPDCFF
                     HVSRPEIDLPDGTGLYDEIVYRTAAANFAAFRDRGTLLPDPEPGLYLYRLTWGAHAQT
                     GLIAVCHADDYAHNVIRKHEKTRQAPEDDRTRHLVAVEAHTGPVLLAYRDQPEVDRLV
                     SGECRVPPLYDVTAADGIRHEIWKARDPAALERLFAAVPAAYIADGHHRAAAAARIAR
                     ERRAAGGPAAEESDWFLAVLFPASRLKILPYNRCVRDLNGLAPADFLRLVGETFEVTP
                     AAHPAPEHPGRVHLFLGNQWHRLSWPSAAMEDPVASLDASVLQDRLLGPVLGIDDPRK
                     NPRIEFVGGIHGTKELEARVRSGRAAAAFSLYPTTVEQLMAVADRGLTMPPKSTWFEP
                     KLRDGLVIHPLKSF"
     gene            complement(2468..5143)
                     /locus_tag="PK634_07330"
     CDS             complement(2468..5143)
                     /locus_tag="PK634_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW97585.1"
                     /translation="MRPVRRPSIRAQALAATLFAGLLPLLFFLVTAEIIAYQSLVRGE
                     IRVLRTLVRVAGHQIGYVMQRAAADLQLLAANPAWSLPGGDVDLMLRDMQQMKTMSRL
                     FSDITVYDRGGGVVASSSYENWENEQYTDCFRKAIEGQSSVSPPGRIIGQDGLFLRFF
                     APITGADGQTRYVLSARCPFDRVAETLRGLRLGQGGYLMLMDTGGNVLDHPDPARALQ
                     KFDPDRPASFWSGNPQGFYRQPDGQRFAYAAMVLGPDHIGSASPWILVGLRPHREILT
                     PLWMGLLLLSGMVFITIAVVYLLGSLQSRRLARPIVEAAAAAQRVGRGDLTVTVPEQG
                     PREIHHLAEAFNRMIREVRQSRADLEHLVEQRTGRLHESQQKLEQISAHLRAAYESIA
                     DGLLMAEWPSGRIVTANQRVEDLFELAPNTVSGSTLDRLAELIRGKLAAGAGDIFHVR
                     QFTENPADIAVEEWELMSPRRQTLSVYSAPATGPGGEVTARLWMFRDLTRQRQLEDEL
                     RQAQKMEAIGRLAGGIAHDFNNLLTGILGNLSMADMELPRASGLRQYLEPARQAARRA
                     AELVNQLLGFSRRSTLQLQACAVNRLARELHELLRHSVNPRIEFRLELQTDLWDVAAD
                     ATQIHQVLMNLSMNSVDAMPSGGRLTIRTRNVRIERDEARQWLDAREGDFVKVSVEDT
                     GHGMAPEVQRHLFEPFFTTKGPGRGTGLGLAMSYGIVKQHGGWITCYSEVRQGSTFSL
                     YLPRLAQPAGAAKPAGPAEPAVRGGRERLLVVDDEAAVREIVRVILHRWGYTVEEAAN
                     GPEALRLVEANPPDLILLDLTMPELSGLETLQRIRVLAPRVPVIISSGYVLEPGAPEL
                     DPSIAPQGIVQKPFEAHSLARTVREVLDRAGAAPS"
     gene            complement(5140..6246)
                     /locus_tag="PK634_07335"
     CDS             complement(5140..6246)
                     /locus_tag="PK634_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOW97586.1"
                     /translation="MNRLTWPGLAVCAAMLAAGCGRSSAPPGTEPPAERLLRVLNWED
                     YIDPALVEQFTERTGIPVDYQTFATQDELVGLIRSDPGRSDLVIVDESVIQTLIDLQL
                     LRTMDASRLAGLRHLDPPGTTLPPDINPDYAAPYHFGCTLLAYRKDQAPEPPAAWEAL
                     WDERYRGRIMMPVDFQEVFAVTLLSLGHPMNSGDPRELEEARERILRQAPLVLEYTDA
                     TTIQEALNSGEAALGVLYNGDAARIAATNSQIGIVLPPEGVPLWVDCFCFPRDARNVE
                     AALAFMDFLMEPDIAAQNAAFLHYATPNRAARALMSPALLEDPAVFPPAAIRARSSYY
                     LKPDVARLRMYNSTAAEVRRLRGALGLGPGTADP"
     gene            6376..7116
                     /gene="pyrH"
                     /locus_tag="PK634_07340"
     CDS             6376..7116
                     /gene="pyrH"
                     /locus_tag="PK634_07340"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOW97587.1"
                     /translation="MAREQKTARSRFKRVLLKLSGEALQDREGGQSLSPAILASITGQ
                     VRAARALGVQVAIVVGGGNIFRGLPGEGHGIDRVTGDYMGMLATMINALAIQATLSRN
                     GVDARAISAIAMPALADTFSRRTAVHHLDKGRVVVFGAGTGNPFFTTDTAAALRASEI
                     GADLLLKATKVDGIYTEDPVKNPKARRFTTLTYQEALQRQLKVMDAAAFSLCQENRIP
                     IIVFNFFKRNELVRALKGEAVGTLVRAG"
     gene            7131..7694
                     /gene="frr"
                     /locus_tag="PK634_07345"
     CDS             7131..7694
                     /gene="frr"
                     /locus_tag="PK634_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOW97588.1"
                     /translation="MESLDDVLLESDDKMTKAVEFLQQELSGLRTGKASPSLVENVQV
                     EYYGTQTRLRQLAGIATPEPRLIVINPYDPTSLQAIERAILAANIGITPINDGRIIRI
                     PIPELSEERRKDLVKVGHRMAEEARVAIRNVRREANDQIKALQKGGKITEDERDEGLK
                     EIQNYTDTYIGKVDALIAAKEKDVLTV"
     gene            7845..9662
                     /locus_tag="PK634_07350"
     CDS             7845..9662
                     /locus_tag="PK634_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97589.1"
                     /translation="MKRILVSLALVLCAVSSLAVVQIQGFPTLYLQITDAVHWAISGD
                     TLLISTGVFNEAVDITGKDLVLDGGYTNDCSYKINDAYSVIDADAFFPGAGHGSALDI
                     SGSIVVLKDLHIRGGEWALGTTAGYGGGLDIRSDSTVTARNCRVYSNTALGKGGGIYV
                     EDSILYLVNTEVASNHTYLASGLLVTPYGWGGGLCAERSRVEAGGDSPFFLNWAASAG
                     GGLCGSESRLYLHDSSVDISDNSASNGGGVAVFGGELEIATSADIHRNVASNNGGGLL
                     LTGGATGILRGASTTVGTKSAGSNRALAGSGGGVYASNALLVVSNAAGVFCNTAASAG
                     GGVFLLGSACVVDNADIGVGGSTNGALAGGGLAVIDSRLEFRGAAAVAGGQAFMGGGL
                     FATNSLLIVGPDAVIGDAQPTNANVALGGGGIYADTCEVQLQGKVWNNASLGGAGMGL
                     MRCSLLASNAQIVGNIGSTNPTAYGGGLAMTGGTGILHSVTIVSNVCYHGGGAALLGD
                     VRAEFWPAAFIAYNRAEADGGGLYCSNANSLVWLDQATIQSNSARRNGGALCVVTGEV
                     QVGSAYLHANSAGADGGALYATNALVTFWSDAEAVAFIT"
     gene            9706..11196
                     /locus_tag="PK634_07355"
     CDS             9706..11196
                     /locus_tag="PK634_07355"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOW97590.1"
                     /translation="MINLNGFTNLYLTSNAASGNGGGAWLMYSFLWSGGRVEIGGNDA
                     AGSGGGVHADSGSFVMLQPSPAGRPNIYNNTAVDNGGGLCVLEAQASLKDVRIGLDFS
                     GNPSANRCLGSAAGDGGGGLALLQSRAWLTNCWVAYNRAEYYGGGIEVSGSRLTMKGN
                     PALLSPGDFHPPNRIFGNVVSNGSLGIGGGLHAVDSTVRIEDALVYSNLALYGGGLHA
                     DAPSTVLVVNAAILLNQASVTGGGVRAYGSITHMDLLHCAVVNNSRGGISVASSAEVS
                     LTNCIVGLNQSEQITAGHSVSYSDVAGGYAGAGNIQLDPFFILSDVYDVRLGYGSPCI
                     NNGAVAAAVAADAIGEPRPFGAGYDMGAYEYNGDTYDTDGERMTDGWEADHSLDPWVG
                     DALDNDDSDEYLNIEEYISDTDPFDGDSHLQITSITNGATRDVGFLSSTARVYALYTT
                     PHMTNDATWTIVGTNLNLRGNGVTYTAIDTNAPAGQAFYRVDVQVP"
     gene            complement(11257..11697)
                     /locus_tag="PK634_07360"
     CDS             complement(11257..11697)
                     /locus_tag="PK634_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97591.1"
                     /translation="MENLPWLARWSLYGLMSLMGVLGLMIGAFQFRTMRGGVFHNCDG
                     SKDDWHEQPTHYGIALADVCVACPVSTLGVILAFAGSRWGFYLLALASFWWLWANVMT
                     TATSLKFHQPKITLMWFFTFPLGALLGLAYLVLTFVHFDALYRP"
     gene            complement(11784..14240)
                     /locus_tag="PK634_07365"
     CDS             complement(11784..14240)
                     /locus_tag="PK634_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97592.1"
                     /translation="MRTNRTSRMSIVIVLAAALTASAQGPNIPNLINYQGRLLNGTNL
                     FNGKVSISFRFFDAPTGGAPACVDSNDVPVADGLYSTYIGNHITWGSLETAMMQTQVW
                     IEVVIGTNTLSPREQMVAVGYARYAAGLPANAVQTHHVGADQIYGFHLASGAVSSNKI
                     NWAAMPAGLQDGDQEGIAAETDPVFDTANHQGDVTGVWTAMKIAPGAVTSNKIDWAAM
                     PAGLQDGDDGYSPLGYDENGAFAAAPQAIGNNAIALGYGAVARADYSAVGGGRNNIIS
                     NNANYSTIAGGDDNRVGDNANYAALAGGVGNRIANEASYASLVGGWGNQVGYNAWYGT
                     IGGGYQNEVSTNAQAATVAGGNNNRIAAEARNAVIAGGLQNRVYASATNAAIGGGSGN
                     MISNVASYATIGGGGGNRIGTNTAYAAIGGGKDNHVNAQARFGFIGGGTLNTMGTGAE
                     AGTIGGGSGNTVDNGATYATVGGGQNNQVGDNSGSAVIAGGSSHSIGNESPGAAIGGG
                     NLNTTGTNCDSTVIGGGQQNDMGNNCGHSVIGGGEDNDLGRGSHHAVVAGGQNNDMAG
                     DAFHSFMGGGWNNKIESSARLSFVGGGEANVIRTNAIHAVISGGDNNVIGTNAGASVI
                     GGGYQNQIGKDAQAATIPGGYLCKVSANANGAFAAGTHAVAAHEGSFVLTDSQMSDHT
                     SAGADTLNTRFQGGIHLNGGTSELVIDNTGIEATGPCRGTYPRPDYDSGWQAISLGGT
                     LALTHSLGGDTDDYVVDIQFNDTDGVQDINQGSGQWTDSADRNVGAFWRKLTATSVEV
                     YRASSDPYADEVRVRIWVYQ"
     gene            14405..16018
                     /locus_tag="PK634_07370"
     CDS             14405..16018
                     /locus_tag="PK634_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97593.1"
                     /translation="MKQILIALVALADSLAGKSAAAPGYGEPISLTTGVVRRVGTVTA
                     LQNAVAAVNAAGVPATILVSNGTYVLTDWALPVTCDGVIIRSLGGDRDAVVIRGPDEG
                     ASASLQHVFWVTASHVTIADLTFGWCLYHGIQAHGETPYNVSHLWVHNCRIVNCNEQF
                     IKGTSIDGDPEGVTDGLVENCLFEFTSGWAYQFYTGGIDAHKAANWTVRDNLFRNIRA
                     TDGLAEHAIHFWKRNTARPQNVAVERNVIVNCDRGIGFGLGGWADGHDGGTSVIRNNV
                     VYNDGAGPNTDVGIGLESATGVKADNNTVVVPYWAPIEFRFAGSSNLVFRNNLAGGAI
                     TARDGAPAPARSNNLESVQAEWFRSLTNGDLRLKPGVTGVLDKGCAIAAFADDVDGDA
                     RPAGAAWDIGADEYDPWTADSNFDGVPDGWYLQHGLNPTSLTVAVEDEDEDEYDNKEE
                     WIALTDPTNGASFFRVAGIGQVSPFTVTLSCSTARVYTLEQAAPPATGDWSRVPGAIH
                     VSGAAHGTLALADTNPAPRRLYRVSVGLP"
     gene            16105..19179
                     /locus_tag="PK634_07375"
     CDS             16105..19179
                     /locus_tag="PK634_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOW97594.1"
                     /translation="MMTIRVFPRPLSVRFGLFIWGAAALLSVAPVRAQDDAYEPNNAT
                     NEATDLSASPGIWLSDNLGLGIQCDSDWYKIAVPAGLERVTVDCLSSAARGNITIALY
                     DSNAVRLAWSDSWEDRDGLEFVVPTNGTYYVLVYGANRCNAYNLQWDRQSPAGGADDA
                     YETNDTASGAFDLKARRGTPLSLIAGLGRQCDPDWYRIYVAPGSERLMAQCVYTNDPG
                     AFGLELRNEMGQQVALAAPPMDRSTLDLRVPVAGTYYLQVPGGAACQEYDLWWTCAIP
                     PNRAGPPVVVALFFNENFVDTSTLTEGEAYNVWKALEAMSVRVNTFTGITEAAFSLAL
                     GGANVLLIPELEGGHLASSLDPATLAILRGFVAGGGCLVIHGQYLGNDAAFVNAVFGW
                     SVQPQGDHSDATSTNNPGATAGTAFEEGPPSLGGENGLYPWLAASLPPGSSSLYQFES
                     GGDADTTVAVIPYGAGRAVFLAWDWYNGAPVGMQDKGWNGILLSALAQCSCEPLLTAE
                     RRSGPDTLVWLDGGFKHTYDIMYTDEDLKGSPAWTPFGILGSRIVTAPLEDFSFTDDY
                     TALTSGGPSATGRRAYRVMRHPAVTAPEVAILLDANYVDMTWGAIPDAEGPNLVAALQ
                     RMRYGISSIAGITAQDFANALRPGGFLLIPELERGNLGADLSAGARAAISNFVQAGGT
                     MIVHADIGASDENLVNTLFGFGLAGIGDMSYMDTTSTAAVAGTEYERCPATLPGIDGL
                     YPWIPGTLPPGASVLYETPANGTNYPSVVVIPQGLGRVVLLAWDWWRAAPLGRQDGGW
                     NEALDSTFRNWSDKNLFVGYYDLQAGQGVVPQHEPIETAGHTGVCLFDLAAEDLAGID
                     VLFAQNPANAGYAEEFTNRLDAIRQAVMGGMTLVFHDRATTNLTAQMLPGGQGIGFTR
                     DMSLPLANDIQILTAGTFVTDGPGGVLNNGSLDNGNASSHGYADEATLPAGAVRILSR
                     GAATEVVTFAYPCGAGWVVYSSIPLDFFLGGGSTNFREIYAPNVVEYGGWLP"
     gene            19281..>19706
                     /locus_tag="PK634_07380"
     CDS             19281..>19706
                     /locus_tag="PK634_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOW97595.1"
                     /translation="MMGKRGGLILAGWLAAGAAGAAELTFTVMAANLSGSDFEYDESA
                     DRVFQGLKPDIVAIQEWTVAGGDRRAFVDRNFGKDFFFCVENEPRDKVPNGVISRWPI
                     LSAGTWDDPQVPDRGFAWARIDLPHVTNDLYVVSVHLHSS"
BASE COUNT         3160 a         6508 c         6808 g         3230 t
ORIGIN      
        1 cgcccaggag ccattttaac aaggacccca ttcagggctt agatgagagt caagccgcca
       61 cgcccaatga tagagcggtg attacaaagc cgattgcctt cattgcgtgt ctcctttttt
      121 cggacaactc cccctcggaa aagttgctcc gatactggag ccttttaagc ccgtcctttg
      181 gctacggtca agttccgaaa tcggagcatg acacatgccg gaaaatatcg tggggacgca
      241 agtgcggcgg ctacgctatc agcaggaact ttctcaagcg ttgttggctg ccaagctgaa
      301 ccggctgggg tgggacatta gccgaggaac actggcaaag attgaagccg gaattcgttg
      361 tatttcagac aaagaagtgt tcctgctggc aaaggccctg aaggtgcctc tcaaccagtt
      421 gtattcaggc gggcgcgcct gatctggtgt ccaacaacct gcgagggaag gagagtcgcc
      481 gccttttttc ggggagacga caactctgcg ccccgcgaag cccccgccgg cggggacaac
      541 gggcacaccc ccgccccccc ttggcgcggt tcacggccac gataccggcc cggcttggcg
      601 cgtcccgttg ccgggttcta gccgccgtta tcctgtgtgt aaaaaaagcc gtcggcgtcc
      661 ggcccttgtc ctgatacctg ccgggagaga tcgggccgat tctctttcac catgcgtatc
      721 cccttgaagc cgctggcctg ctacaatgcc cgctagtggc ctttaaacgc ccgtggcggg
      781 gcgatctgta gggttaaata gaatggtgaa ctaccgttca gtttccatgc tgcaacattt
      841 tttcgggggc tcgtgtcttg ctccccggcc cgctggccgc ctgtcctgaa cacttccgcg
      901 aacgtcggca accgtcacgg ctttcaatgc ccatgataaa aaaagtgata taagaagtgg
      961 cctgaatcag agccatgatt aaaagaaaag ccaatattca caagggtttt gtgagattgg
     1021 cggggacgac gggactcgaa cccgcaacac ccagatcgac aatctggtac tctaaccaat
     1081 tgagctacgt ccccggccca cgcaaaacgg aagcagtcta gtcggcgcgg cccgtttttc
     1141 aagcggaaac cacgcgcttt ttttcagaac gatttcagag ggtggatcac cagcccgtcg
     1201 cgcagcttcg gttcgaacca cgtgctcttg ggcggcatgg tcaggccgcg gtccgcgacg
     1261 gccatgagct gctccacggt ggtcgggtag agcgagaacg ccgcggcggc gcgcccgctc
     1321 cggacgcgcg cctcgagctc cttcgtgccg tggatcccgc cgacgaactc gatgcgcggg
     1381 ttcttgcgcg ggtcgtcgat gccgagcacc ggcccgagca ggcggtcctg gagcacgctg
     1441 gcgtcgagcg aggccaccgg gtcctccatc gcggcggacg gccaggagag ccggtgccac
     1501 tggttgccga ggaagaggtg cacgcggccc gggtgctccg gcgcggggtg ggcggccggc
     1561 gtcacttcga acgtctcgcc gacgaggcgg aggaagtccg ccggggcgag gccgttgagg
     1621 tcgcgcacgc accggttgta gggcaggatc ttcaggcggc ttgccggaaa gagcaccgcg
     1681 aggaaccagt cgctctcctc cgcggccggg ccgccggccg cgcgccgctc gcgggcgatg
     1741 cgggccgcgg cggccgcccg gtggtggccg tcggcaatgt acgcggcggg gaccgcggcg
     1801 aagaggcgct cgagggccgc cgggtcccgg gccttccaga tttcgtgccg gatgccgtcg
     1861 gcggccgtca cgtcgtacag gggcgggacg cggcattccc cgctcacgag gcggtcgacc
     1921 tcgggctggt cgcggtaggc gagcaggacc ggcccggtgt gggcctcgac ggccaccagg
     1981 tggcgcgtcc ggtcgtcctc cggggcctgc cgcgttttct cgtgcttgcg gatcacgttg
     2041 tgcgcgtagt cgtcggcgtg gcagacggcg atcaggccgg tctgcgcgtg ggcgccccag
     2101 gtcagccggt agaggtacag gccgggctcc gggtcgggaa ggagcgtgcc gcggtcgcgg
     2161 aacgcggcga agttggcggc cgcggtccgg tacacgatct cgtcgtaaag cccggtgccg
     2221 tcgggcaggt cgatctccgg ccgggaaacg tggaagaagc aatccgggtt gccctccgcc
     2281 tgccggcggg cctcggcggt gtcgacgacg tcgtacggcg ggctggccac gcgggcggcg
     2341 cgatcggcgg gcgggcgaag ggcctggaag gctttgatgc gcatcgaaac aatccttgtt
     2401 ggtgcggttc agctcactcg gcggatgtcg tatagcacgg gcgcggcccg cttgtccacc
     2461 gccggcctca cgagggcgcg gcgcccgccc ggtcgagcac ctcccggacg gtgcgggcga
     2521 gcgagtgggc ctcgaagggt ttctgcacga tcccctgcgg cgcgatcgac gggtccagct
     2581 cgggcgcccc gggctccagg acgtagccgc tggagatgat gaccggcacg cgcggcgcca
     2641 ggacgcggat ccgctggagc gtctcgagcc cggacaactc gggcatggtc aggtccagca
     2701 ggatgagatc gggcggattg gcctcgacga ggcgcaacgc ctccggcccg ttggcggcct
     2761 cctccacggt gtagccccag cgatggagga tgacgcggac gatctcgcgg acggcggcct
     2821 cgtcgtccac caccagcagc cgttcccgcc cgccgcgcac ggcgggttcc gcggggccgg
     2881 ccggcttcgc ggcgccggcc ggctgggcca ggcggggcag gtagaggctg aaggtggagc
     2941 cctggcggac ctccgagtag caggtgatcc acccgccatg ctgcttgacg atgccgtagg
     3001 acatggccag gccgaggccg gtgccgcgcc cggggccctt ggtggtgaaa aacggctcga
     3061 agaggtgccg ctgcacctcc ggggccatgc cgtgcccggt gtcctcgacc gagaccttca
     3121 cgaagtcgcc ctcccgcgcg tcgagccact ggcgggcctc gtcgcgctcg atgcggacgt
     3181 tgcgcgtccg gatcgtcagc cggccgccgg acggcatggc gtccacggag ttcatggaga
     3241 ggttcatgag gacctggtgg atctgcgtcg cgtccgcggc gacgtcccac aggtcggtct
     3301 gcagttccag gcggaactcg atccgcgggt tcacgctgtg gcggagcaac tcgtgcaact
     3361 cgcgggcgag gcggttgacg gcgcaggcct ggagctgcag ggtgctgcgc cgggagaagc
     3421 cgagcagctg gttcaccagc tcggcggcgc ggcgggcggc ctggcgggcc ggctcgaggt
     3481 actggcggag gccggaggcc cggggcagct ccatgtcggc catggacagg ttgccgagga
     3541 tgccggtcag gaggttgttg aagtcgtgcg cgatgccgcc ggcgaggcgg ccgatggcct
     3601 ccatcttctg cgcctgccgg agttcgtcct cgagctgccg ctggcgggtc aggtcccgga
     3661 acatccacag gcgcgcggtg acctcgccgc cgggcccggt ggcgggggcg gagtaaacgg
     3721 aaagggtctg gcggcgcggc gacatcagct cccattcctc gaccgcgatg tcggcggggt
     3781 tctcggtgaa ctgccggacg tggaagatgt cgccggcccc ggcggcgagc ttgccgcgga
     3841 tcagctccgc cagccggtcc aacgtgctgc cggagaccgt gttcggcgcg agttcgaaga
     3901 ggtcctcgac ccgctggttg gccgtcacga tgcggccgga gggccactcc gccatgagga
     3961 ggccgtcggc gatggactcg taggcggcgc gcaggtgcgc gctgatctgc tccagcttct
     4021 gctgcgattc gtgcaggcgc cccgtccgct gttcgaccag gtgttcaagg tccgcgcggc
     4081 tctggcgcac ctcccggatc atgcggttga acgcctcggc gaggtggtgg atttcgcggg
     4141 gcccctgctc ggggacggtg acggtgaggt cgccccggcc cacgcgctgg gccgccgccg
     4201 cggcctcgac gatggggcgc gccagccgcc ggctctgcag gctgccgagc aggtagacga
     4261 cggcgatggt gatgaacacc atgccgctca acagcagcag ccccatccac agcggggtca
     4321 ggatctcgcg gtgggggcgg agccccacga ggatccaggg gctggcggag ccgatgtggt
     4381 cgggcccgag caccatggcg gcgtacgcga aacgctggcc gtcgggctgc cggtagaatc
     4441 cctgggggtt gccggaccag aaactcgcgg gccggtccgg gtcgaatttc tggagcgcgc
     4501 gggccgggtc ggggtgatcg aggacgttgc cccccgtgtc catgagcatc aggtacccgc
     4561 cctggcccag ccgcaggccg cggagcgtct ccgcgacgcg gtcgaagggg cagcgcgcgc
     4621 tcaacacgta gcgggtctgg ccgtccgccc cggtgatcgg cgcgaagaag cgcaggaaca
     4681 ggccgtcctg cccgatgatc cggccgggcg gcgagacgct gctctgcccc tcgatcgcct
     4741 tccggaagca gtccgtgtac tgttcgttct cccagttctc gtagctcgag gaggcgacca
     4801 cgccgccgcc ccggtcgtag accgtgatgt cggagaacag ccggctcatg gtcttcatct
     4861 gctgcatgtc ccgcagcatg agatccacgt cgccgcccgg caggctccag gcggggttgg
     4921 cggcgagcag ctgcaggtcg gcggcggccc gctgcatcac gtagccgatc tggtggccgg
     4981 ccacgcggac cagcgtgcgc agcacccgga tctccccgcg caccagtgac tggtaggcta
     5041 tgatctccgc ggtgacgagg aagaacagca gcggcagcag tccggcgaac agcgtggcgg
     5101 cgagggcctg cgcgcggatc gaggggcggc ggaccggcct catgggtccg ccgtccccgg
     5161 cccgagcccg agcgctcccc ggaggcggcg gacctccgcg gccgtggagt tgtacatgcg
     5221 gaggcgggcc acgtccggct tgaggtagta gctgctccgg gcgcggatcg cggcgggcgg
     5281 gaacaccgcc gggtcctcga ggagggcggg ggacatcagg gcgcgcgccg cgcggttcgg
     5341 cgtggcgtag tggaggaagg cggcgttctg cgcggcgatg tccggctcca tcaggaagtc
     5401 catgaacgcc agcgccgcct cgacgttccg cgcgtcgcgc gggaagcaga agcagtccac
     5461 ccagagcggc acgccctcgg gcgggagcac gatcccgatc tggctgttcg tggccgcgat
     5521 ccgggccgcg tccccgttgt acagcacgcc cagcgcggcc tcgccggaat tcagggcctc
     5581 ctggatggtc gtggcgtccg tgtactcgag gaccaggggg gcctggcgga ggatccgctc
     5641 gcgggcctcc tcgagctcgc gcggatcgcc ggaattcatc gggtggccga gcgacagcag
     5701 cgtcaccgcg aacacttcct ggaaatcgac gggcatcatg atgcgtccgc ggtagcgctc
     5761 gtcccagagc gcctcccagg cggcgggcgg ctcgggcgcc tggtccttgc ggtaggccag
     5821 cagcgtgcag ccgaaatggt acggcgccgc gtagtccggg ttgatgtcgg ggggcagcgt
     5881 cgtgccgggc ggatcgagat ggcgcagccc ggccaggcgg gaggcgtcca tggtccggag
     5941 caactggagg tcgatgagcg tctggatgac gctttcgtcc acgatgacca ggtcgctgcg
     6001 gccgggatcg gagcggatca ggccgacgag ttcgtcctga gtggcgaagg tctggtagtc
     6061 cacggggatg cccgtccgct cggtgaactg ctccaccagc gccggatcga tgtagtcctc
     6121 ccagttcagg acccggagca ggcgctcggc cggcggctcg gttcccggcg gggcggaact
     6181 ccgcccgcac ccggccgcca acatcgcggc gcacacggcc aagcccggcc aggtcagtcg
     6241 gttcatggtg tcggcatgcc ttgctggctt ggatagtacg ggcggcgcga gcggcggtca
     6301 agacggcgcg ccgctgcttt acacgggggg cgggttcctt taccatgccg gggagtcagg
     6361 cgaaaggagc ggtgcatggc ccgggagcag aaaacggcgc ggtccaggtt caagcgggta
     6421 ttgctcaagt tgagcggcga ggcgctccag gaccgcgagg gcgggcagag cttgagcccc
     6481 gccatcctcg cgtccatcac gggtcaggtc cgcgccgcgc gggcgctggg cgtgcaggtc
     6541 gccatcgtcg tcggcggcgg gaacatcttc cgcggcctgc cgggcgaggg gcacggcatc
     6601 gaccgcgtca cgggcgacta catgggcatg ctcgcgacca tgatcaacgc cctggcgatc
     6661 caggcgacgc tctcccgcaa cggcgtggac gcccgcgcga tcagcgccat cgccatgccc
     6721 gccctggcgg ataccttctc gcgccgcacc gccgtccacc acctcgacaa gggccgcgtc
     6781 gtcgtcttcg gcgccggcac gggcaacccg ttcttcacca ccgacaccgc cgccgcgctg
     6841 cgcgcgagcg agatcggcgc cgacctgctg ctcaaggcga ccaaggtgga cggcatctac
     6901 accgaggatc ccgtgaagaa cccgaaggcg cgccggttca cgacgctgac ctaccaggag
     6961 gcgctccagc gccagctcaa ggtcatggac gccgccgcgt tctcgctctg ccaggagaac
     7021 cggataccga tcatcgtctt caacttcttc aagaggaacg aactggtccg cgcgctcaag
     7081 ggggaggccg tcggcaccct cgtccgcgcc ggttgaacaa ggagaccgcc atggaatcgc
     7141 tcgacgacgt gctgctggaa tcggacgaca agatgaccaa ggccgtggag ttccttcagc
     7201 aggagctctc ggggctgcgc accggcaagg cctcgccgag cctggtggag aacgtccagg
     7261 tggaatacta cggcacgcag acgcgcctgc gccagctcgc gggcatcgcc acgcccgagc
     7321 cgcgcctgat cgtgatcaac ccgtacgacc ccacctcgct gcaggccatc gagagggcga
     7381 tcctcgcggc caacatcggc atcacgccga tcaacgacgg ccggatcatc cgcatcccga
     7441 tccccgagtt gagcgaggag cggcgcaagg acctggtcaa ggtgggccac cgcatggcgg
     7501 aggaggcgcg cgtcgccatc cgcaacgtcc ggcgcgaggc gaacgaccag atcaaggccc
     7561 tgcagaaggg cggcaagatc accgaggacg agcgggacga ggggctcaag gagatccaga
     7621 actacaccga tacctacatc ggcaaggtgg acgccctgat cgccgcgaag gaaaaagacg
     7681 tcctgacggt gtagaaagcc gagcgtccag cgctcaccat ccaacgccca agtgttggaa
     7741 gttggacgct ggttgttgga cgttgaaaag gccttaacat tttgtattca tggccctatt
     7801 ataacgttta agtctcgggt cggctgcaaa aaggaggtcg tctcatgaag cggatactgg
     7861 tttccctggc gctggtgctg tgtgccgttt cctccctggc ggtggtgcag atccaggggt
     7921 tcccgacctt gtaccttcaa attaccgatg ccgtgcactg ggcgatcagc ggagacaccc
     7981 ttctcatctc cacgggcgtg ttcaacgagg ccgtggacat caccggcaag gacctggttc
     8041 tggacggcgg ctataccaac gattgctcgt acaagatcaa tgatgcgtac agcgtcattg
     8101 atgccgatgc cttcttcccg ggggcagggc atggttcggc gctggacata tcaggaagca
     8161 tcgtcgtgct gaaagacctt cacatccggg gaggcgaatg ggcgctgggc acgacggccg
     8221 gctatggcgg cgggttggac atccggtccg acagcacggt cacggcccgc aattgccggg
     8281 tctacagcaa cacggccctg ggcaagggcg ggggcatcta cgtcgaggat tccattctct
     8341 acctcgtgaa caccgaggtg gccagcaatc acacgtacct cgcgtcgggt cttttggtga
     8401 ccccctacgg ttggggcggc ggcctctgcg ccgagcgcag ccgggtggag gcgggcgggg
     8461 actcgccctt cttcctgaac tgggccgcca gcgcgggcgg cgggctgtgc ggctcggaaa
     8521 gccggctgta cctgcatgac tcctccgtgg atatcagcga taacagcgcg tccaacggcg
     8581 gcggggtcgc cgtcttcggc ggcgaactgg agatcgccac cagcgcggac atccaccgca
     8641 atgtcgcctc caacaacggg ggcggactcc ttctcacggg cggggccacg ggcatccttc
     8701 gcggcgcgag caccaccgtc ggcaccaagt ccgccggctc caaccgggcc ctggccggat
     8761 caggcggcgg cgtctacgcc tccaacgcgc tcctggtggt cagcaacgcg gcgggcgtgt
     8821 tctgcaacac ggccgccagc gccggcggcg gggtgttcct cctcggcagc gcgtgcgtcg
     8881 tggacaacgc ggatatcggc gtgggcggca gcaccaacgg ggccttggcg ggcggcgggc
     8941 tggcggtgat cgactcccgg ctggaattca ggggcgccgc ggccgtggcg ggcggccagg
     9001 ccttcatggg cggcgggctc ttcgctacca attccctgtt gatcgtgggg cccgacgccg
     9061 tcatcgggga cgcgcagccg acgaatgcca acgtggccct gggcggcggc ggcatctatg
     9121 cggacacgtg cgaggtgcaa ttgcagggta aggtctggaa caacgcgagc ctgggcgggg
     9181 cggggatggg tctgatgcgc tgctccctgc tcgccagcaa cgcgcagatc gtcggcaaca
     9241 tcgggtccac gaatccgacg gcctacggcg gaggcctggc gatgactggc ggcaccggca
     9301 tcctgcacag cgtgacgatc gtcagcaatg tttgctatca tggcggcggg gcggccctgc
     9361 tgggcgacgt gcgcgcggaa ttctggcccg cggctttcat cgcctacaac cgcgccgagg
     9421 ccgatggcgg cggcctgtat tgcagcaacg ccaacagcct cgtatggctg gaccaggcga
     9481 ccatccagag caactccgcg aggcggaacg gcggggcgct gtgcgtggtg accggggagg
     9541 tccaggtcgg cagcgcgtat ctccacgcga actccgccgg cgccgacggc ggagccctgt
     9601 acgccaccaa tgccctggtc acgttctggt ccgatgcgga ggccgtcgcc ttcatcacgt
     9661 aaaaattcgg cgcaacgggg cggcggtctg gccgccagcc attcggtgat caatctcaac
     9721 gggttcacca acctgtatct cacctccaac gccgcctccg gcaacggcgg cggagcctgg
     9781 ttgatgtaca gcttcctctg gagcgggggc cgggtcgaga tcggcggcaa cgacgccgcc
     9841 ggctcgggag gcggagtcca tgccgacagc ggcagctttg tcatgctgca accctctccg
     9901 gctggccgcc ccaacatcta caacaacacg gccgtggaca acggcggcgg cctctgcgtt
     9961 ttggaggctc aagccagcct gaaagatgtc cgcatcggcc tcgatttcag cgggaatccc
    10021 tccgccaacc ggtgcctggg atcggcggcc ggcgatggcg gcggggggct ggccctgctg
    10081 cagtcccgcg cctggctgac caactgctgg gtggcgtaca accgggccga atactacggc
    10141 ggaggcatcg aggtgtccgg ctcgaggctg accatgaaag gcaatccggc gctcctgtcg
    10201 ccgggtgatt tccatccgcc gaacaggata ttcggcaacg tggtgtccaa cggcagcctg
    10261 ggcatcggcg gcggcttgca cgccgtggac agcaccgtcc gcatcgagga tgccctcgtg
    10321 tacagcaacc tcgcgctgta cggcggggga ttgcatgccg atgcgcccag cacggtcctg
    10381 gtcgtcaacg cggccatcct gctgaaccag gcctcggtca ccgggggcgg cgtgcgggcc
    10441 tacggcagca ttacgcatat ggatcttctc cactgcgcgg tcgtgaacaa tagccgcggc
    10501 ggcatcagcg tggcctcttc cgcggaagtg tccctgacca actgcatcgt gggcctgaac
    10561 caatccgaac aaatcaccgc cggccattcg gtcagttaca gcgacgtggc cggcggttat
    10621 gcgggcgccg gcaatattca gctcgacccc ttcttcatct tgagcgatgt gtacgatgtg
    10681 cgcctcggtt acggttcccc ctgcatcaac aacggcgcgg tggcggcggc ggtggcggcc
    10741 gacgccatcg gcgagccccg cccgttcggc gccggttacg acatgggcgc ctacgagtac
    10801 aacggcgaca cctacgacac cgacggcgag cgcatgacgg atggctggga ggcggatcac
    10861 agcctcgatc cctgggtggg tgacgcgctc gacaacgacg attcggacga gtacctgaac
    10921 atcgaggaat atatcagcga tacggatccc ttcgacgggg acagccacct gcaaatcacc
    10981 tcgatcacca acggcgcgac gcgcgatgtc ggcttcctgt cttccacggc ccgggtctat
    11041 gccctctaca cgacgccgca catgaccaac gacgcgacgt ggacgatcgt ggggaccaac
    11101 ctcaacctgc gcggcaatgg cgtgacatac acggcgatcg acaccaacgc gccggcgggc
    11161 caggccttct accgcgtgga cgtgcaggtg ccgtaggagg atgagggcgg gacccctcgt
    11221 cccgcgccat cagggcccgg cgtctccgtt ccgacgtcac ggcctgtaca gcgcgtcgaa
    11281 atggacgaag gtcaggacga gataggccag gccgagcagc gcgccgaggg ggaacgtgaa
    11341 gaaccacatc agggtgatct tcggctggtg gaacttcagg ctggtggccg tggtcatgac
    11401 gttggcccag agccaccaga agcttgccag cgccaggagg tagaagcccc aacgcgagcc
    11461 cgcgaacgcg aggatcactc cgagcgtgct gaccgggcag gcgacgcaga catcggcgag
    11521 ggcgatgccg tagtgcgtgg gctgctcgtg ccagtcgtcc ttcgagccgt cgcagttgtg
    11581 gaacacgcca ccccgcatcg tgcggaactg gaacgcgccg atcatcaggc ccagcacacc
    11641 catcaggctc atcagcccgt acaggctcca ccgcgctagc cagggcaggt tttccatgtt
    11701 tcctcggctt cccccgcgtc aaggttcgac cacgatccag tattcgcccg gcgggctgga
    11761 acggtaaagc cgccgggtct ccgtcattga taaacccaga tgcgcacgcg gacttcatca
    11821 gcataagggt cactggacgc ccggtacact tccacactcg tggccgtcag tttgcgccag
    11881 aacgcgccga cgttgcggtc cgcggagtcc gtccactgcc cggacccctg attgatgtcc
    11941 tggacgccat cggtatcgtt aaactggatg tccacgacat aatcatccgt atctcctccc
    12001 aggctgtgcg tgagcgcaag ggttccgccc aacgagatgg cctgccatcc gctgtcgtag
    12061 tccggccggg ggtacgtgcc gcggcagggg ccggtggcct cgatgccggt gttgtcaatg
    12121 accagctcgc tggttccgcc gttcaggtgg atgccgccct ggaagcgggt gttgagcgtg
    12181 tcggcgcccg cgctggtgtg atcggacatc tggctgtcgg tcagcacgaa cgagccctca
    12241 tgcgcggcga cggcatgcgt gccggccgcg aaggcgccgt tggcgttggc ggagaccttg
    12301 cacagataac cgccggggat ggtcgcggcc tgcgcgtcct tgccgatctg gttctggtag
    12361 cctccgccga tgacggaagc cccggcgttg gtgccgatga cattattgtc accccccgat
    12421 atcacggcgt gaatcgcatt ggtacgaatc acattcgctt ccccgcctcc cacaaaagac
    12481 aatcgcgcac tgctttcgat cttgttgttc cagccgccgc ccatgaacga atggaacgcg
    12541 tcgccggcca tgtcgttatt ctggccgccg gccaccacgg catggtggga tccccgtcca
    12601 agatcattgt cctcgccgcc gccgatcacg gagtgcccgc agttgttgcc catgtcgttc
    12661 tgttggccgc cgccgatgac ggtcgagtcg caattggtcc cggtcgtgtt taagttgccg
    12721 ccgccgatgg ccgcgccggg cgattcattt ccaatgctgt gggatgatcc tccggcaatg
    12781 accgcggatc cgctgttgtc gcccacctgg ttgttttgcc cgccgccgac cgtcgcatag
    12841 gtggccccgt tatcaaccgt gttgccggag ccgccgccga tcgtgccggc ttccgcgccg
    12901 gtccccatgg tgttgagcgt gcccccaccg ataaagccaa aacgggcttg cgcattcaca
    12961 tggttatcct taccgccgcc gatggcggca taggcggtgt tcgtgccgat gcgattgcct
    13021 ccgccgccgc cgatggtggc atagctggcc acgttgctga tcatgttgcc ggagccgccc
    13081 ccgatcgcgg cgtttgtcgc ggaagcatag acccggttct gaaggccccc ggcgatcacg
    13141 gcgttccgcg cctcggccgc gatgcggttg ttgttgccgc ccgccacggt ggcggcttgc
    13201 gcgttcgtgc tgacctcgtt ctggtagccg ccgccgatcg tgccgtacca ggcgttgtat
    13261 cccacctggt tgccccaacc gccgacgagg gaggcatagc tggcctcgtt ggcaatccgg
    13321 ttgccgaccc cgcccgcaag ggccgcgtag ttggcgttgt cgccgacgcg gttgtcgtca
    13381 ccgccggcga tcgtcgagta gttggcgttg ttcgagatga tgttgttccg gccgccgccg
    13441 accgcgctgt agtcggcccg ggccaccgcg ccataaccca gggcgatggc gttgttgccg
    13501 atcgcctgcg gggccgccgc gaaggccccg ttctcgtcgt atcccagcgg cgagtacccg
    13561 tcgtccccgt cctgcagccc cgcgggcatg gcggcccagt caatcttgtt gctggtcacg
    13621 gccccggggg cgatcttcat ggccgtccat acgccggtca cgtcaccctg gtggttcgcc
    13681 gtgtcgaaca cgggatcggt ttccgccgcg atcccctcct gatccccgtc ctgcaagcct
    13741 gcaggcatcg cggcccagtt gatcttgttg ctgctcaccg ccccggaggc caaatggaat
    13801 ccgtagatct gatccgcccc gacgtggtgc gtctgcaccg cgttggcggg gaggccggcc
    13861 gcgtaacggg cgtagcccac ggccaccatc tgttcccgag gcgacagggt gttcgttccg
    13921 atcaccacct cgatccagac ctgcgtctgc atcatggcgg tctccaggct gccccaggtg
    13981 atgtggttcc cgatgtaggt cgagtacagg ccgtccgcga ctggcacgtc attggagtcc
    14041 acgcaggccg gggccccgcc cgtgggggcg tcgaagaatc ggaacgagat ggataccttg
    14101 ccgttgaaga ggttcgtccc gttcagcagc cggccctggt aattgatcag gttcggaatg
    14161 ttcggcccct gcgcggacgc ggtcagcgcg gcggcgagga caataacgat ggacattctg
    14221 ctcgtgcgat tggttctcat gatcccctcc aagcctcctg tcatggcttc cattctaccg
    14281 gtcaggtcgt ggcccgtcat gggaaaatcg catcctgctc cccgatgcga caaataagcc
    14341 catcccgacg accagagcca tgggaaatcg atccccggca tgggatgtgc ttggaggaga
    14401 tggaatgaag cagatcctca tcgcgctggt ggcgctggcg gacagccttg ccgggaagtc
    14461 cgcggccgcg ccgggctatg gcgagccgat ctcgctcacc accggcgtgg tccggcgggt
    14521 ggggacggtc accgcgctgc agaacgccgt cgccgcggtc aatgccgcgg gcgtcccggc
    14581 tacgatcctg gtctccaacg ggacctatgt cctcaccgac tgggcgctgc cggtcacgtg
    14641 cgacggcgtg atcatccgca gcctgggcgg cgaccgcgac gccgtggtga tccgcgggcc
    14701 ggacgagggc gcgtccgcct cgctccagca cgtcttctgg gtgaccgcct cgcacgtcac
    14761 catcgcggac ctgaccttcg gctggtgcct ctaccacggc atccaggcgc acggcgaaac
    14821 gccgtacaac gtctcccatc tctgggtcca caactgccgg atcgtcaact gcaacgagca
    14881 gttcatcaag gggacctcga tcgacggcga tcccgagggc gtgacggacg gcctggtgga
    14941 gaactgcctg ttcgaattca cgagcggctg ggcctaccag ttctacacgg gcgggattga
    15001 cgcccacaag gcggccaact ggaccgtgcg cgacaacctg ttccggaaca tccgcgcgac
    15061 cgacggcctc gccgagcacg cgatccattt ctggaagcgc aacacggcgc gcccgcagaa
    15121 cgtggccgtc gagcgcaacg tcatcgtgaa ttgcgaccgg ggcatcgggt tcgggctcgg
    15181 cggctgggcg gacggccacg acggcggaac gagcgtgatc cggaacaacg tcgtctacaa
    15241 cgacggggcg ggcccgaaca cggacgtcgg catcggactg gaatccgcga cgggcgtgaa
    15301 ggcggacaac aacacggtgg tcgtcccgta ctgggcgccg atcgagttcc ggttcgcggg
    15361 cagctcgaac ctggtctttc gcaacaacct ggccggcggg gccatcacgg cccgcgacgg
    15421 cgccccggcg ccggcgcggt cgaacaacct ggagagcgtc caggcggaat ggttccggag
    15481 cctgacgaat ggcgacctgc gcctcaagcc gggcgtcacg ggtgtcctgg acaagggttg
    15541 cgcgatcgcg gccttcgcgg acgacgtgga cggcgacgcc cggcccgcgg gcgcggcgtg
    15601 ggacatcggc gcggacgagt acgatccgtg gacggccgac tccaacttcg acggcgtgcc
    15661 ggacgggtgg tacctgcagc acggcctgaa cccgacgagc ctgaccgtgg cggtcgagga
    15721 cgaggacgaa gacgagtacg acaacaagga ggaatggatc gcgctgaccg atccgaccaa
    15781 cggcgcgtcg tttttccgcg tcgcgggaat cggtcaggtg agcccattta ccgtcacgct
    15841 gtcctgctcg acggcgcggg tgtacacgct ggagcaggcc gccccgccgg ctacgggtga
    15901 ctggtcgcgg gtgcccggcg ccatccatgt ttccggcgcg gcccacggta cgctggccct
    15961 ggcggacacc aacccggccc cccgccggct gtaccgcgtg agcgtgggcc tgccgtaagc
    16021 ttttcgagtg tacacaccgg ggaactccgt gatttactat ttcttcgtcg tgccgggggg
    16081 aggccgtctc gcatggaggg aatcatgatg acgatccgcg tattcccgcg cccgttatcc
    16141 gttcgcttcg gcctgttcat ctggggggcg gccgcgctgc tgtccgttgc gccggttcgg
    16201 gcgcaggacg atgcctatga gcccaacaac gcgacgaacg aggcgacgga cctgtccgcg
    16261 agtcccggga tctggctttc cgacaacctc ggcctcggca tccagtgcga cagcgactgg
    16321 tacaagattg ccgtgccggc cggcctcgag cgggtgacgg tggactgcct ctcctccgcg
    16381 gcgcgcggca acatcaccat cgccctgtac gacagcaatg ccgtgcggct ggcctggtcc
    16441 gacagttggg aggaccgcga cggattggag ttcgtcgtcc cgacgaacgg cacctactac
    16501 gtgctggtct acggggccaa ccggtgcaac gcctacaacc tgcaatggga ccggcagtcg
    16561 ccggcgggcg gggcggacga tgcgtacgaa accaacgaca cggcctcggg cgccttcgac
    16621 ctgaaggcgc gccgcgggac gcccctctcg ctgatcgccg ggctgggccg gcaatgcgac
    16681 ccggactggt accggatcta cgtcgcgccg ggctccgagc ggctgatggc gcaatgcgtg
    16741 tataccaacg atcccggcgc gttcggactc gaactgcgga acgagatggg gcagcaggtg
    16801 gcgctggccg cgccgccgat ggatcggagc accctggatc tccgggtgcc ggtggccggg
    16861 acgtactacc tccaggtgcc gggcggggcg gcatgccagg agtacgacct gtggtggacg
    16921 tgcgcgatcc cgcccaaccg ggccggcccg ccggtcgtcg tggcgctctt ctttaatgag
    16981 aacttcgtgg acacgtccac cctgaccgag ggcgaggcgt acaacgtctg gaaggccctg
    17041 gaggccatgt cggtccgggt gaacaccttc accggcatca ccgaggcggc gttttccctc
    17101 gccctgggcg gcgccaacgt cctgctgatc cccgaactcg aggggggcca cctggcgtcg
    17161 tcccttgatc cggctaccct ggccatcctc cgcggcttcg tcgccggggg agggtgcctg
    17221 gtcatccatg gtcaatactt gggaaacgac gccgccttcg tgaatgccgt gttcggatgg
    17281 tccgttcagc cgcaaggcga tcattccgac gcgacctcca cgaacaatcc cggcgcgacg
    17341 gccgggaccg cgttcgagga gggtccgccg agcctcggcg gggagaacgg actgtatccg
    17401 tggctcgcgg ccagcctgcc cccgggcagt tccagcctct accagtttga gtccggcggc
    17461 gacgcggaca ccaccgtggc cgtcatcccg tacggcgccg gccgcgcggt cttcctggcg
    17521 tgggattggt acaacggcgc gccggtcggg atgcaggaca agggctggaa cggcatcctg
    17581 ctctccgccc tggcccagtg ctcctgcgag ccgctcctga cggccgagcg gcggtccggc
    17641 cccgacacgc tggtctggct cgacggcggc ttcaaacaca cctatgacat catgtacacc
    17701 gacgaggacc tgaaggggag cccggcctgg acgcccttcg gcatcctggg ctcgcggatc
    17761 gtgacggcgc cgctagagga tttttccttc accgacgact acacggccct gaccagcggc
    17821 gggccgtccg cgaccgggcg tcgcgcgtac cgggtgatgc gccatcccgc ggtgaccgcc
    17881 cccgaagtcg cgatcctgct cgatgcgaat tacgtggaca tgacgtgggg cgccatcccg
    17941 gatgcggaag gcccgaacct ggtggcggcg ctgcagcgca tgcgctacgg gatctcgtcc
    18001 atcgccggca tcaccgcgca ggacttcgcc aacgccctcc ggccgggcgg gttcctgctc
    18061 attccggaac tggagagggg caacctgggc gccgatctct ccgcgggcgc gcgggcggcg
    18121 atcagcaact tcgtccaggc aggcggcacg atgatcgtgc acgcggatat cggcgcctct
    18181 gacgagaacc tggtcaacac actgttcggc ttcggcctcg ccggcatcgg cgacatgagc
    18241 tacatggaca ccaccagcac cgcggcggtc gccggcacgg agtacgagcg gtgtccggcg
    18301 acgctccccg gcatcgacgg gctctatccc tggattcccg gtacgctgcc cccgggcgcg
    18361 tccgtcctgt acgagacgcc ggccaacggc accaactatc ccagcgtcgt cgtcatcccg
    18421 cagggcctgg gccgggtggt gcttctcgcc tgggactggt ggcgtgccgc gccgctgggc
    18481 aggcaggacg gcggctggaa cgaggcgctc gactccacgt tcaggaactg gagcgacaag
    18541 aacctcttcg tgggctacta tgacctgcaa gccgggcagg gcgtcgtccc ccagcatgag
    18601 cccatcgaga cggccgggca cacgggcgtg tgccttttcg atctggccgc cgaggacctg
    18661 gccgggatcg acgtcctctt cgcgcagaat cccgccaacg ccggctatgc cgaggaattc
    18721 accaaccgcc tcgacgccat ccggcaggcc gtcatgggcg gcatgacgct ggtcttccac
    18781 gaccgggcga cgaccaatct caccgcccag atgctcccgg gcggccaggg catcggcttc
    18841 acccgcgaca tgtcgctgcc gctggccaac gacatccaga tcctcacggc cgggacgttc
    18901 gtcacggatg gccccggcgg ggtgctgaac aacggctcgc tggacaacgg gaatgcctcc
    18961 tcgcacgggt acgcggacga ggccacgctg ccggcgggcg cggtccgcat cctgtcgcgc
    19021 ggcgcggcga cggaggtcgt caccttcgcc tatccctgcg gcgccgggtg ggtcgtgtac
    19081 tcctcgattc ccctggactt cttcctgggc ggcggctcca cgaacttccg ggagatctac
    19141 gcgccgaacg tcgtggagta cggcggctgg ctgccctgat ccccgcggcc tgaaaataaa
    19201 tagtcgcctc ccggcggccg gccggggtag gctggccggg tcttttccaa tgattggaaa
    19261 agcgcggaag gatttttcca atgatgggaa aacgcggcgg gctgatcctg gcggggtggc
    19321 tggcggcggg cgcggcgggc gcggccgagc tcacgttcac cgtcatggcc gccaaccttt
    19381 ccggctccga tttcgagtac gacgagtccg cggaccgggt cttccagggc ctgaaaccgg
    19441 acatcgtcgc gatccaggag tggaccgtgg cgggcggcga ccggcgggcg ttcgtggacc
    19501 ggaatttcgg gaaggacttc tttttctgcg tggagaacga gccgcgcgac aaggtgccca
    19561 acggcgtcat cagccgctgg ccgattctgt ccgccgggac atgggacgat cctcaggtgc
    19621 cggatcgcgg ttttgcttgg gcgcggattg atctgccgca cgtcaccaac gatctctacg
    19681 tggtcagcgt ccatctgcac agcagc
//
LOCUS       DAOOTK010000050        19262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1959_length_19262_cov_4.615349, whole genome shotgun sequence.
ACCESSION   DAOOTK010000050 DAOOTK010000000
VERSION     DAOOTK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19262
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1959_length_19262_cov_4.615349"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PK634_07385"
     CDS             <1..747
                     /locus_tag="PK634_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97596.1"
                     /translation="LPAGRWSEKAFCGVFADWLFSSPADPGLWDRMVDALIESPSMAA
                     SGLARIVLQQDDSREVEKHWELWLARQAQVRRIGERSAADTLAEFEARLTARPGEWPE
                     ISARHLPESLTMEELIPYRAEPWLPAVALRLQVGLGTQGFGLDAGLRRVRDLYLAYLS
                     ALAPGRPPGLFGWLRSSRPGEARLRELLRAADEALAAYKEEQRDRARFVDAAEAEFSP
                     KAAEPGAMEREWPRAEMQRFVDEASREAPR"
     gene            923..2299
                     /gene="argH"
                     /locus_tag="PK634_07390"
     CDS             923..2299
                     /gene="argH"
                     /locus_tag="PK634_07390"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOW97597.1"
                     /translation="MVRNRLTTVGAIDPEVLAYTAGDDIALDLALAEADALGTAAHVT
                     MLSRMPVDPPVITAAECRKVVRALIGIVGKARAGGLRIGLADQDIHLAVERMLTEKLG
                     EAGRKVHTGRSRNDQVALDLRLHAKVRLIEVIESAGGLARVLVEFARRHAAVPMVGRT
                     HMQPAMPSSVGLWADAHAEGLLDDAALLLAVYSLNDQCPLGSAASYGVPLPIDRQLVA
                     DLLGFSRPIHNVLYANNARGRNEQAILSALAGIMGTLSRLAQDLILWSMPEFGYFTLP
                     SGFCTGSSIMPQKRNPDVLELVRARAAAVASSAALVGELVRAAPSGYNRDIQEAKGPF
                     LKGLSVTRDTLRILGRLVGGIEVHPEALRAAFTPDVFATDRALELVAGGMPFREAYHH
                     VKAHLDELGRMDPDAAIARKTHLGAPAGLDFQADLARCRDLARFAQDERRKLEKAFSR
                     LMGVAWRL"
     gene            2334..4067
                     /locus_tag="PK634_07395"
     CDS             2334..4067
                     /locus_tag="PK634_07395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOW97598.1"
                     /translation="MTEEQSGEEAPKEEVFSTTMRIDITPHLKEQLASSFGPGPAQTR
                     RTGRVTIKIPKKTGDASDLGSSDFQEILQNVYDATLITDQNGRIASVNPRAIQFFRGE
                     RAEIVGRNIVELISGASEDLVPTIRKNLENDRYTLIQAYCLRGDGTIFPAEISANHLH
                     LSARDYLSFFIRDISWRKEAEERLRTGYTAIQNSGDGIAIADLEGAISFCNPAMTALC
                     GEAATEHMIGRNLREFLAEPDKADEILQNVSGHNVWVGELEMRRVDGATFYAQGSVAP
                     NVNADDELTGMVLSVLDITNLKQAQYQLECFARELQEKNTLLESDQIMAREIQQAFLP
                     RQHPVFPRGAKAEDSILKVGHLYLPSGAVGGDFFDMLVLSDSQAGLFISDVAGHGMRA
                     ALVVATIRGLMEELAPIAGDPAAFLTQLNRAYTGIFSQTEQLTFTTACYLVLDLKTGR
                     MQYTTAGHPFPFHLRRSAGTVSMLPAGEGGKGPALGLFDTASYGSATGILEPGDVLLF
                     YTDGLSEAEGTNRELFEDHEMVASLQKLLPKEPGALLTDLVAAARQFKGATDFEDDVC
                     LLAVELARLLS"
     gene            complement(4101..4182)
                     /locus_tag="PK634_07400"
     tRNA            complement(4101..4182)
                     /locus_tag="PK634_07400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4146..4148),aa:Leu,seq:tag)
     gene            complement(4227..5153)
                     /locus_tag="PK634_07405"
     CDS             complement(4227..5153)
                     /locus_tag="PK634_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOW97599.1"
                     /translation="MAASGKMLGIVAGVVALILLVACGGLVFLNQQQAKEAAGLKDAF
                     LKAASAAGIADLTPESLKDPAALQRVQDTITAQGQELATTKDALTTAGTEAANAKSEA
                     AAQIQKAQEQAAQVAELTTQLAGKDGEITTAKAAADKAAQERQAAQQEAAKLKTDLDA
                     ATKKVQELEAAAAPAPTAEGAEAAAEGGATEAVEAAAEGAEEAAPAAPAEEPEPEGRG
                     IGKSEMFRSIKYTAADQVLVLKLADGQKLTYKDVPEPVYGGMLNSADIDKYYRFKIQG
                     VFSSTPEDKQAIRDFWRNINNRPVRGDLKVIE"
     gene            5342..6262
                     /locus_tag="PK634_07410"
     CDS             5342..6262
                     /locus_tag="PK634_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97600.1"
                     /translation="MTRRKKSAGPAGPGGEAEPPIAAWTQREHPEWLDEQAWAEWRAL
                     CALGLCRHAVQESLASFAGFHFRRLVSRYAFRVNAPGEARALTDRDGWHLFETHLTAR
                     QTREGKRYKDWLFARVPPDANSPMQAVAGGAVLLMRDAAREYLAREFAPAGQVSLSSP
                     VNSAESGRLALEDLLPDAASPADEVARREYEALAREHAEEWFAALGARDRMLLLARHM
                     GLPLNHPVVEQAAGCRKSKACASLRNFVENLAADLRRQFADDPAESVRLLATLTLEAL
                     NQRIDQWGRQEPRCADLFSLASRHEETTPG"
     gene            6240..6938
                     /locus_tag="PK634_07415"
     CDS             6240..6938
                     /locus_tag="PK634_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97601.1"
                     /translation="MKKLPPVKQAAASSARRQWLQQWLHEWTVDLALRGDGEEPPPDA
                     VGRPPEAGPSAAPGQVRLLFPCTGPEGARLCYLAVLREVRPGHFLAAPFGRFSVPATP
                     GEWRTPRAEAPLRVLELWNARVLSGAALAASWTVDELLPREREDALAVYQAWYARQAP
                     PGEPDIRTGPPLIHPLDPRHEYMEEECAWMDGVAARFFSRAEKASAFPASAPDELPLA
                     AEPRKPYRARRRKK"
     gene            7499..7936
                     /gene="ssb"
                     /locus_tag="PK634_07420"
     CDS             7499..7936
                     /gene="ssb"
                     /locus_tag="PK634_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOW97602.1"
                     /translation="MVAMNKVLLMGNLTRDPEIRKTPGGASVLDLGLAINERYKGKDG
                     KVVENVCFTDVVVWGRQAESCARYLTKGAPVLVEGSLRFDQWKTEDGQNRSRLKVHAF
                     HVQFLGGKEPGVEDPSDEAAEQEPARVERKPTERVAVPRAAVR"
     gene            8505..8999
                     /locus_tag="PK634_07425"
     CDS             8505..8999
                     /locus_tag="PK634_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOW97603.1"
                     /translation="MPREIAASKKAGGRSRKPAKGAKHVAAGRGGAKPVLLAGGNPQI
                     AKADGDAPVQAYLAAMPGWESDVGRRLDALVVSNVPGVCRAVRWNSPFYGVQGRGWFM
                     GFHVFTRYIKVSFFRGASLRPAPSGGTGKDARWIDIHESDLDEAQMANWIRQAAALPG
                     WGKA"
     gene            9085..9702
                     /gene="wrbA"
                     /locus_tag="PK634_07430"
     CDS             9085..9702
                     /gene="wrbA"
                     /locus_tag="PK634_07430"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOW97604.1"
                     /translation="MGANVYVVFYSTYGHVYKMAEAVAAGVREVAGAEARLVQVPELM
                     PEAALVKSGAKAARQAFAHVPVIQPDQLAEADAIIFGTPTRFGNMSAQMRNFLDQTAG
                     LWLKGALVGKVGSVFASTGTQHGGQETTITSFHTSLFHHGMIVVGVPYAEQRLLNMQE
                     ITGGTPYGATTLAAGDGSRQPSENELAIARFQGRHVAQIASRLRG"
     gene            complement(9719..10912)
                     /locus_tag="PK634_07435"
     CDS             complement(9719..10912)
                     /locus_tag="PK634_07435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOW97605.1"
                     /translation="MAGDSITRRSFLKALTLAGTGSLLSAGLPASAAGEGGGTVPRRI
                     LGRTGVHISSLCLGGIFDVPANQHILHRALQLGVTCWDTAEGYHGDRSEAGFGQFFEK
                     QPAARRDVFLITKTDAHHPEGLSNSLKRSLEKLRTDHIDLYLIHDLRGVETLTDDVRD
                     WAEAAKGSGRIRYFGFSTHRNMAQQLRQAAALGWLDAVLSACNFRLLQAADMQAALEQ
                     CGAAGLGVIGMKAQGGGPVPADAGSDLAGQFLGRGFTAAQAKLKAIWENPAIASICSQ
                     MHVARNLEENSAAAMDRTALSSADRARLALFAQATCEGYCAGCERFCGPALGSRIPIS
                     DLMRGLMYARDYGNPERARDCLRAVGPGIRDAICSTDFTLAERACPQGLPIGRLAREA
                     LELLA"
     gene            complement(10975..12771)
                     /locus_tag="PK634_07440"
     CDS             complement(10975..12771)
                     /locus_tag="PK634_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOW97606.1"
                     /translation="MKITWVRRISQVFFLLLFLWFCLVTTVGEEWWRLRGWPVNWFLQ
                     LNPLTALGVVLGTGSLPAGLEWSLLVIALTVILGRFFCGWVCPFGTLHQAIGWLGLRR
                     AAPAEQCLRRAFRPAQRIKYYLLAFLLAAAAGGLLAPAVGRLAGQSARLESIREFAAG
                     SLQTGWLDPISFMHRAVNLVILPLADRGIHVLWTGARLAQGAALIGGLFLAALLVNLW
                     IPRFYCRYVCPLGALYGVLGRFALWRVGKRQRDCSNCLKCEAACEGACQPAGRIRTHE
                     CVLCMNCLHACPDDLVGYRLARSASGERAAPDLTRRGVIASLLAGFAVAPLGRLAGGR
                     DPRRIRPPGAGPEREFLTRCLRCGQCMRICPTNILHPALTEAGLEGFGTPVAKFSLGI
                     GGCQLRCAACSQICPTGAIRPLTLNEKIGRGAFRERGPVRMGTAFVDRGRCLPWAMNR
                     PCIVCEENCPVSPKAISIQEIFEPLPGRRREIARGDELTLFFEGETDAAARVSDDLYC
                     RIAGDFDAEPRRIIECGPAQWSIDPNHPWESPPPKGSVAELLVLLQRPVVDPERCIGC
                     GVCESVCPLATVRAIRVTADGESREARSGFLL"
     gene            complement(12768..13760)
                     /locus_tag="PK634_07445"
     CDS             complement(12768..13760)
                     /locus_tag="PK634_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOW97607.1"
                     /translation="MSRPPLDPERREFLARGLKAAAALAATGLAGHALGWRTGRADRE
                     EVHDWPVPDFSQPAADPRLAIVTGPDRTTNLRKAMEALGGWARFVKPGDGVLLKVNAA
                     FASPATLGATTHPDLVAEAVAQCLRAGASRVVVTDHPIHDQASCFEWSGIGAAARRAG
                     AELWVPREDDFAPVTLPGGRLLRHWPFLKGPFKGIRRLIGLTPVKDHYRSGASLTLKN
                     WYGLLGGRRSVFHQDIHTLIAELAQLVRPTLVILDGTTTMIRNGPTGGSLADLKATDT
                     LVVGTDPVAVDAFGVTLLGREASDLPHLARAAAAGAGTASYELLNPVRIQAGAS"
     gene            complement(13757..14755)
                     /locus_tag="PK634_07450"
     CDS             complement(13757..14755)
                     /locus_tag="PK634_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97608.1"
                     /translation="MARKTGWGETLLGLAILAALAGVGTAVYFEQFRPHPAAYSADKP
                     DYLPPPAPRPAAAARPSRVNPPEGMTALGPSEYFDPDTLYEKIDGRAELYLPAGFRSL
                     ECRRLGIPGDEDSVVETFLYDMGSPRSAYSVFSQQQREDAEPRDFTRYAYLAENALFF
                     CHGSYYLEIIAGTAAASAREALRKAAAQVMADLPAEPADLPELARLPEAHRLAGHPRL
                     YRTDGLGFAPLTDLFVADYQIGEFQLSGFVCARGSEAEAAKLAQAYQQFIMDDGAMLL
                     PPIPELPDARVLDFYGTWQVLFTRGPYFAGVHQAGDRLSAEQLALLLDRQLSEAAP"
     gene            14905..15726
                     /locus_tag="PK634_07455"
     CDS             14905..15726
                     /locus_tag="PK634_07455"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOW97609.1"
                     /translation="MKKAGWLGWVAIVLAGAGTAAAAPVRARHSEAELTAEQAAIRAG
                     ETVWLALAIRLDPGWHTYWLNPGDSGMAPRLAWSLPEGFSAGALAAPPPARFESEGLV
                     SLGYGERVVFLVPITAPAGWGGGPATVGLKATWLVCSDLCLPESATFSLELNGAAAAA
                     PEGLFAEARARLPLPPDGWTLRALRRGREVHVSVEPPAGSGAESPMVFFPARADEFTY
                     DTATVPQQGPALRLQISPLAETFPEKLMGVLVAHGTALALDIPIETPVEAEGVVP"
     gene            15723..16328
                     /locus_tag="PK634_07460"
     CDS             15723..16328
                     /locus_tag="PK634_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOW97610.1"
                     /translation="MKGCARTAVILMVVGLALIVWAEISPDGAAPDFTLPGIDGVPHS
                     LSSARGKYVVLEWFNYDCPFVRKHYGGGNMQALQKKYTEAGAVWFSICSSAPGKQGHY
                     PAEKMAELAKQRNVASTAVLLDPDGRVGRLYGAKTTPHIFIIDPEGKLIYQGAVDSVP
                     SADPADITSAVNYVRAALDAALAGQPVEKKVTAPYGCSVKY"
     gene            complement(16479..17618)
                     /locus_tag="PK634_07465"
     CDS             complement(16479..17618)
                     /locus_tag="PK634_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOW97611.1"
                     /translation="MIMLLIFPIVLLSYAAMHAYMGIKLVRAFPRLRLFTAWLLIVFT
                     GLFAAPFLARWFGNRGHPLLAELAGAAGYTWIAIVFWFFSVGLLLDAWNLLVRLASLF
                     SARAARRRVSPRGTVGIAGIVAAAAMAWGLAEARWLRVETIVLRSDRLPAGTTYRVAQ
                     ISDLHLTARRGGWLVDTVVSAVHALKPDLIVSTGDLIDAPAAQLRDKAGPLRRLAAPG
                     GRYAVLGNHEYFAGVSEALAFHQAAGFTLLRGAAAEPLPGLWLAGVDDPAGSFTHQPY
                     FISERLLAAARPADRFVVLLKHQPLVSRPEKAWFDLQLSGHTHGGQMFPFEWLLKPIY
                     PLRRGLHEVAPGRWLYVNRGAGTWGPPMRLLARPEITLIVIEGTR"
     gene            18043..18642
                     /gene="thpR"
                     /locus_tag="PK634_07470"
     CDS             18043..18642
                     /gene="thpR"
                     /locus_tag="PK634_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOW97612.1"
                     /translation="MNQDINEAGDEVLRAFIALEISDEVRAALDRLQRDLMRGEARVG
                     WVTPARIHLTLAFLGDIFAAQLPGLADALDAVAEGCPPFAFDVAGTGAFGPPKSPRVV
                     WAGVEEPTGALVRLQAAAAVAVTGLGFRIEERAFHPHLTLGRVRDRRGAADLTSRLES
                     FRTLRCGRVEVRRVLLLQSVLSSQGAEYRVRHSSELKGA"
     gene            18689..>19262
                     /gene="recA"
                     /locus_tag="PK634_07475"
     CDS             18689..>19262
                     /gene="recA"
                     /locus_tag="PK634_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOW97613.1"
                     /translation="MPAALSAVLAQIQKEFGEGAIMRLGKEEAHVQVPSISTGALTLD
                     LALGIGGVPRGRIIEIFGPESSGKTTLVLHIIANAQKNGGVAAFIDAEHALDPAYARK
                     IGVNLDDLLVSQPDSGEEALTIADQLVRSNSVDVVVIDSVAALAPRAELEGAMGDSHV
                     GLQARLMSQAMRKLTGILNKSKTCCIFTNQI"
BASE COUNT         3089 a         6371 c         6790 g         3012 t
ORIGIN      
        1 ctacccgcgg gtcgctggag cgagaaggct ttttgcggcg tgtttgcgga ctggctgttt
       61 tcatccccgg ccgatccggg cctgtgggat cgcatggtcg atgccctgat cgaatcgcca
      121 tcgatggcgg cctccggtct cgcccggatc gtgttgcagc aggatgactc gcgcgaggtg
      181 gagaagcact gggagctctg gctggcgcgc caggcgcagg tccgccgcat cggggaacgc
      241 tccgcggcgg acacgctggc ggagttcgag gcccggttga cggcgcggcc gggcgagtgg
      301 cccgagatct cggcgcgcca cctgccggaa agcctcacga tggaggagtt gatcccgtat
      361 cgggcggagc cctggctgcc ggcggtggcc ctgcggctgc aggtcggcct gggcacgcaa
      421 ggcttcgggc tggatgccgg cctgcggcgg gttcgtgatc tgtacctggc gtatctttcc
      481 gccctggcgc cgggtcggcc gccgggcctg ttcggctggc tgcggtcgtc ccgccccggg
      541 gaggcgcgcc tgcgcgagct gctgcgggcc gccgacgagg ctcttgcggc ctacaaggaa
      601 gagcagcgcg atcgcgcccg atttgtcgac gcagcggagg cggagttcag tccgaaggcc
      661 gcggaacccg gcgcgatgga gcgggaatgg ccccgtgcgg agatgcaacg cttcgtcgac
      721 gaagccagcc gggaagcgcc ccgttgagga gccgacgccg caacggcgcc tctcgcgcgt
      781 gtatcacagt ttataaactg tgatacatgg ccggcgccgc ggatccggcc cgcggatcgg
      841 gtttccggcc ggaaggtcgt gcgcgcttga ctccggacgg gcagtctata ggctggaccg
      901 aagataaacc agacggtgac ccatggtccg gaatcggcta acgacggtcg gcgcaatcga
      961 cccggaagtg ctcgcgtaca cggccgggga tgacatcgcc ctggacctgg ccctggccga
     1021 ggccgatgcg ctcgggaccg ccgcccacgt caccatgctt tcccgcatgc cggtcgatcc
     1081 gcccgtgatc acggccgcgg aatgccggaa ggtggtccgc gccctcatcg gcatcgtcgg
     1141 caaggcgcgg gccggcgggt tgcggatcgg gctggcggac caggatatcc atctggcggt
     1201 cgagcggatg ctcaccgaga agctcggcga ggccggccgc aaggtgcata ccggccggag
     1261 ccggaacgac caggtggccc tcgatctccg gctccatgcc aaggtgcggc tgatcgaagt
     1321 gatcgagtcg gcgggcggac tggcccgggt tcttgtcgag tttgcccggc ggcacgccgc
     1381 cgtgccgatg gtggggcgga cccacatgca gcccgccatg ccgtcgtcgg tgggcctgtg
     1441 ggcggatgcg cacgcggaag ggctgctgga cgacgcggcc ctgctgctgg ccgtgtactc
     1501 gctgaacgac cagtgcccgc tgggctccgc ggcgagctac ggcgtcccgc tgccgatcga
     1561 ccggcaactc gtggccgacc tgctgggatt cagccggccg atccacaatg tcctgtatgc
     1621 gaacaatgcc cggggccgga acgaacaggc catcctgtcc gcgctggcgg gcatcatggg
     1681 caccctgtcg cggctggcgc aggacctgat cctgtggagc atgccggagt tcggctactt
     1741 cacgctgccc tccggtttct gcacgggcag cagcatcatg ccccagaaac ggaaccccga
     1801 cgtgctggaa ctggtgcggg cccgggccgc ggcggtcgcg tcctcggccg ccctcgtcgg
     1861 cgagctggtc cgcgcggcgc cgtcgggcta caaccgggac atccaggaag ccaagggccc
     1921 gttcttgaag ggcctctcgg tcacccggga tacgttgcgg attctcgggc ggctggtcgg
     1981 cggcatcgag gtccacccgg aggcgctgcg ggcggccttc acccccgacg ttttcgcgac
     2041 agaccgggcg ttggaactgg tggccggcgg catgccgttc cgggaggcct accatcacgt
     2101 caaggcccac ctggatgagc tgggccggat ggatcccgac gcggcgatcg cgcggaaaac
     2161 gcatctcggc gcgccggccg gcctggattt ccaggccgac ctggcccggt gccgcgatct
     2221 ggcgcggttc gcgcaggacg agcggcggaa actcgaaaag gcattctcgc ggttgatggg
     2281 cgtggcatgg cggctgtgaa atcccgggcc ccgggcggcc ggacggagtg cgcatgacgg
     2341 aagagcaatc aggcgaagag gccccgaagg aagaagtgtt ttccaccacg atgcggatcg
     2401 acatcacccc gcatctcaag gagcagctgg cctcctcgtt cggccccggc cccgcgcaga
     2461 cgcgccgcac cggccgcgtg accatcaaga tccccaagaa gaccggggac gcctccgacc
     2521 tcggcagctc cgacttccag gagatcctcc agaacgtcta cgacgccacg ctgatcaccg
     2581 accagaacgg ccggatcgcc agcgtgaacc cgcgggccat ccagttcttc cgcggggagc
     2641 gtgcggagat cgtgggccgc aacatcgtgg agctgatctc cggcgcgagc gaggacctgg
     2701 ttcccacgat ccgcaagaac ctcgagaacg accgctatac cctgatccag gcctattgcc
     2761 tccggggcga cgggacgatc tttcccgccg agatctccgc caaccacctc cacctctccg
     2821 cccgggacta cttgagtttc ttcatccgcg acatctcgtg gcgcaaggag gccgaggagc
     2881 gcctgcgcac cgggtacacc gccattcaga actccggcga cggcatcgcc atcgccgacc
     2941 tcgagggggc catcagcttc tgcaaccccg cgatgaccgc cctgtgcggc gaagcggcca
     3001 ccgagcacat gatcgggcgg aacctgcggg agttcctcgc ggagccggac aaggcggacg
     3061 agatcctgca gaacgtcagc gggcacaacg tctgggtcgg cgagttggag atgcggcgcg
     3121 tcgatggcgc gacgttctat gcccagggct ccgtcgcccc gaacgtgaac gccgacgacg
     3181 agcttaccgg catggtgctc tccgtcctcg atatcaccaa cctcaagcag gcccagtacc
     3241 agctcgaatg cttcgcccgc gagctccagg agaagaacac cctgctcgag agcgaccaga
     3301 tcatggcacg ggagatccag caggccttcc tgccccggca gcatcccgtc tttccgcgcg
     3361 gcgcgaaggc cgaggacagc atcctgaagg tgggccacct ctatctcccc agcggcgccg
     3421 tcgggggcga tttcttcgac atgctggtgc tttccgacag ccaggcgggc ctgttcatct
     3481 ccgacgtggc cggccacggc atgcgcgccg ccctcgtcgt agcgaccatc cggggcctga
     3541 tggaggaact ggcgcccatc gccggcgatc ccgccgcgtt cctgacccag ctgaaccgcg
     3601 cctatactgg catcttcagc cagacggaac agctgacctt cacgaccgcc tgctatctcg
     3661 tgctcgactt gaagaccggc cggatgcagt acacgaccgc gggccatccg tttccgttcc
     3721 acttgcggcg cagcgccggc accgtcagca tgctgcccgc cggggaaggc ggcaaggggc
     3781 ccgccctcgg cctcttcgac acggcctcgt acgggtccgc cacggggatc ctcgagccgg
     3841 gcgacgtcct gctgttctat accgacgggt tgagcgaggc ggagggcacc aaccgcgaac
     3901 tcttcgagga ccacgagatg gtggcctcgc tgcagaagct gttgccgaag gagcccgggg
     3961 cgctgctgac cgacctcgtg gccgccgcgc gccaattcaa gggcgcgacc gatttcgaag
     4021 acgatgtttg cctgctggcc gtggaactcg cccggctgct ttcctgatcc ggaaaccccg
     4081 cgccgccggc cggggtgggg tgggcgagcc gggagttgaa cccggacccc tcacgggaca
     4141 ggatcctaag tcctgcgcgt ctgccagttc cgccactcgc ccgttgaatg ccgaatcctg
     4201 acgccggcct cgcgccggcg tcatcgctat tcgatgacct tcaggtcgcc gcgaaccggc
     4261 ctgttgttga tgttgcgcca gaagtcccgg atcgcctgct tatcctccgg cgtgctggag
     4321 aagacgccct ggatcttgaa gcggtagtac ttgtcgatgt ccgcggaatt cagcatgccg
     4381 ccgtagaccg gctcggggac gtccttgtat gtcagcttct gtccgtccgc gagcttcagg
     4441 accagcacct ggtcggcggc cgtgtacttg atggagcgga acatctccga cttcccgatg
     4501 ccccggcctt ccggctccgg ctcctcggcg ggtgccgccg gcgcggcctc ttcggcgcct
     4561 tctgccgcgg cttccacggc ctccgtcgcg ccgccttctg ccgccgcttc ggcaccctct
     4621 gccgtggggg cgggcgccgc cgccgcttcc agttcctgca ccttcttcgt cgccgcatcc
     4681 aggtcggtct tcagcttggc ggcttcctgc tgcgcggcct gcctttcctg cgcggccttg
     4741 tcggcggcgg ccttcgcggt cgtgatttcg ccgtccttgc cggccagctg cgtcgtgagt
     4801 tcggcgacct gggcggcctg ttcctgggcc ttctggatct gggcggccgc ttccgacttg
     4861 gcgttcgcgg cttccgtgcc cgcggtcgtc agcgcgtcct tcgtggtcgc gagttcctgg
     4921 ccctgtgccg tgatcgtgtc ctgtacccgc tgcagggcgg cagggtcctt gagcgactcg
     4981 ggcgtcagat ccgcgatgcc cgcggcggac gccgccttga ggaacgcatc cttcagcccg
     5041 gcagcttcct tggcctgttg ctggttcagg aacaccagcc ccccgcacgc caccagcagg
     5101 atcaacgcca ccacgcccgc cacgatgccc aacattttgc ccgaagccgc catacgagcc
     5161 tccttccgat tatggttccg tccccgagat ggaatcgaat atccgggcca acatcaacct
     5221 ttttctggaa aacgcaatcg gcgccttttt ttgttgtgga atgagcgcga ccttctacct
     5281 ttctatggat aggaaagatc attcgcgacc gaggggacga aaggaagcga gggggcgtgt
     5341 cgtgacgcgt cggaaaaaga gcgcgggccc ggccggaccg ggcggcgagg cggagccgcc
     5401 gattgcggcc tggacgcagc gcgaacaccc ggaatggctg gatgagcagg cctgggccga
     5461 gtggcgcgcg ttgtgcgcgc tcggtctgtg ccgccacgcc gtccaggagt cgctcgcctc
     5521 ctttgccggc tttcacttcc gccgcctggt gagccgctat gcgtttcgcg tcaatgcccc
     5581 gggcgaggcc cgcgccctta cggaccggga cggatggcat ctttttgaga cccacctgac
     5641 cgcccgccag acgcgggagg gcaagcggta caaggactgg ctgttcgccc gtgttccacc
     5701 tgatgcgaat tcgcccatgc aggccgtcgc gggaggcgcc gtgctgctga tgagggatgc
     5761 cgcccgcgag tacctggccc gcgagttcgc gccggctggg caggtctcgt tgagcagtcc
     5821 cgtgaattcc gcggagtccg gccgcctggc gctggaagac ctgttgccgg atgccgcgag
     5881 cccggccgac gaagtggcgc gccgcgagta cgaagccctg gcccgcgaac acgcggagga
     5941 atggttcgcg gcgctgggcg cccgcgatcg catgttgctc ctggcgcggc acatgggcct
     6001 gccgttgaac catccggtgg tcgaacaggc tgcaggctgc cggaaaagca aggcctgtgc
     6061 ctcgctgcgg aacttcgtgg agaacctggc ggccgacctg cgccgccagt ttgccgacga
     6121 tccggcggag tcggtccgcc tgctggccac gctgactctc gaggcgttga atcagcgcat
     6181 cgatcaatgg ggccggcagg aaccccgatg cgccgacctg ttctcgctgg catcccgtca
     6241 tgaagaaact acccccggtt aaacaggcag cggcatcctc cgcccgccgg cagtggctgc
     6301 agcagtggct gcacgaatgg acggtggatc tcgccctgcg gggcgatggg gaggagcctc
     6361 cgcccgatgc ggtgggccgt ccgcccgagg ccgggccgtc cgccgcgcct gggcaggtga
     6421 ggctgctgtt tccctgtacc ggcccggagg gcgcgcgctt gtgttacctg gcggtcctgc
     6481 gcgaggtgcg gccggggcat ttcctcgcgg ctccgttcgg ccgtttctcc gtgccggcca
     6541 cgcccggcga gtggcgcacg ccgcgcgcgg aagccccgct gcgcgtcctc gagctctgga
     6601 acgcccgcgt cctgtccggc gcggcgctgg ccgccagttg gacggtggat gaacttctgc
     6661 cgcgggagcg ggaggatgcg ctggctgtgt atcaggcctg gtacgcgcgt caggccccgc
     6721 ccggcgagcc ggatatccgg acgggcccgc ccctgatcca tcccctggat ccgcgacacg
     6781 agtacatgga ggaggagtgc gcgtggatgg acggagtcgc ggcgcgtttt tttagccgtg
     6841 cggagaaagc gtccgccttt cccgcttcgg ccccggacga actgccgctg gcggcggagc
     6901 cgcgcaaacc ctatcgtgcg cgaaggcgga aaaaatagga attcccgatc cggaacggat
     6961 ctgaaaaacg agtagtgggc ttaacggaag acaccgcgtt gtcgggccgc gcgggatttc
     7021 cggcaaagcg gatttggcgc tggcaatggg ttggtcacta cgcgtaatcc ttcctaatcc
     7081 cggccctggc gcgatgattc gtcccggctt gaattcggca tgaaccgcgg cgcgctgcgg
     7141 agaggaatgt gtcttggtca gacgggcgga aaacaaggca atgaccaggc ggaacattct
     7201 tttctgctcg actccgagcc cgcctgtccc cggtcggctc gctgctcttt ccttgtgttg
     7261 gcatcgcgct ctgcgatctc cgtggtgaat cattcggctt cgcagtcccc gattcccagc
     7321 gggctgcggt cttcagcgcg ggccgcgtcc gcctccaata ggatgcggca aatctccgcg
     7381 ccgtgtactt ttttaaaaaa agcatggaat ggctggaagc ggcttccttt ctaaggatga
     7441 ggccgggcga gtacgtaccg gccgcgcaaa caggtcaacg aaagaaggag gacaggtcat
     7501 ggtggcgatg aacaaggtat tgctgatggg caacctgacg cgcgatccgg agatacggaa
     7561 aacgcccggg ggagcctcgg ttttggacct cgggctcgcg atcaacgaaa ggtacaaggg
     7621 caaggacggg aaggtggtgg agaacgtctg ttttacggat gtggtggtct gggggcggca
     7681 ggccgagtcg tgcgcgcgct atctgaccaa gggcgcgccc gtgcttgtgg aggggagcct
     7741 tcgcttcgac cagtggaaga ccgaggacgg tcagaaccgg tcgaggttaa aagtccatgc
     7801 tttccacgtg caattcctcg gcggcaaaga gcctggcgtc gaggatccat ccgatgaggc
     7861 ggccgagcag gagccagcca gggtggagcg caaacccacc gaacgggtag cggttccgcg
     7921 cgccgccgtc cggtaggggc ggattcagaa acgccatggc cgcggcgtta tgcctgaccg
     7981 cgccgggcgg acgctacgga ccggcaggtt gcgtgatggt gcggcgacag cggccctgag
     8041 atgggtgaat aggctccggt ccggattggc gatgaccgtc acgcggtctg cgggttaagc
     8101 gcctggtgcg cgggcggcaa ggccgtccgc gcgccgcgcc gacgcgcgag gcggcgggcc
     8161 cggccgcgtg aggtcgggca ggagcgtcgg tgttgaagtg ttcctggccg cgtttcgcgt
     8221 ccaatacggg gatacgacgg gcgtggcgag tttgattccg acctgcatgg ccggtcacgg
     8281 tcgcaagcgg accggggaac tgacttgggc ggggacgtgg catttccacg cagcgcgccg
     8341 gacatgacgc gagggtcgat gcgcgcgttt gcgccgaggc gacgcgcgcc atcaactgaa
     8401 ccggtcctcc ggttgaagag tccatataat attgtatctt ttcctctgca cgtttgggcg
     8461 ccagaagaaa cggataagaa ggggaagaaa aacgggctga ccatatgcca cgagaaattg
     8521 cagcgagtaa aaaagccggc ggcaggtcca gaaaacccgc caagggagcg aagcacgtgg
     8581 ccgccgggcg gggtggcgcg aaaccagtgc tccttgcggg aggcaatccg cagattgcga
     8641 aggcggatgg ggacgcccct gtgcaggctt acctggcggc catgccgggc tgggaaagcg
     8701 atgtcgggcg ccgcctggat gcgctggtcg tgagcaacgt gccgggtgtt tgcagggccg
     8761 tgaggtggaa ttctcccttc tacggggtcc agggccgggg ctggttcatg ggatttcatg
     8821 ttttcactcg ctatatcaag gtatccttct tccggggagc gtcgctgcgg cccgctccat
     8881 ccggaggcac gggcaaggac gcgcgctgga tcgatatcca cgagagcgat ctcgacgagg
     8941 cgcagatggc gaactggatc cggcaagcgg ccgccctgcc cggctggggc aaggcctgac
     9001 cgcgatttcc gggaggacat gtgaggtggg cggcgcaggg aacaaggggg caagagggcg
     9061 gtgtgtaaaa agaaagggaa gaatatgggc gcgaatgtat atgtcgtgtt ttacagcacg
     9121 tatgggcatg tctacaaaat ggccgaggcg gtcgcggccg gcgtccggga agtggcggga
     9181 gcggaggcga ggcttgttca ggttcccgaa ctgatgccgg aggcggcgct ggtgaagagc
     9241 ggcgcgaagg cggcgcggca ggcctttgcc catgtgccgg tcatccaacc ggatcaactg
     9301 gcggaagccg acgcgattat cttcggaacg cccacgcggt tcggcaacat gagcgcgcag
     9361 atgcgcaact tcctggacca gacggccggt ttgtggctga aaggcgccct ggtcggcaag
     9421 gtcgggagtg tgttcgccag cacgggcacg cagcatggcg gccaggagac cacgatcacg
     9481 agtttccata ccagtctgtt ccatcacggc atgatcgtcg tgggcgtccc gtacgccgag
     9541 cagcggttgc tgaacatgca ggagatcacg ggcggcacgc cctacggcgc gaccacgctg
     9601 gcggccgggg acggctcgcg acagccaagc gaaaacgagc tggccatcgc ccgtttccag
     9661 gggcgccacg tggcgcagat agcgtcccgg ctccggggtt gagccccgcc gctcccggtc
     9721 aggccagcag ctcgagcgcc tcgcgggcca ggcggccgat cggcagcccc tgcgggcagg
     9781 cccgttctgc cagcgtgaag tcggttgagc aaatcgcgtc ccggatgccg ggcccgacgg
     9841 cgcgcaggca gtcgcgggcc cgctccgggt tgccgtaatc ccgcgcgtac atcagccctc
     9901 gcatgaggtc ggagatcggg atgcgggagc ccagggccgg tccgcaaaat cgctcgcaac
     9961 ccgcgcaata tccttcgcag gtggcttgcg caaaaagcgc cagccgcgcc cggtcggcgc
    10021 tcgacaaggc cgtacgatcc atggccgccg cggaattttc ctccagattc cgggccacgt
    10081 gcatctgcga gcagatgctg gcgatggcgg gattctccca gatggctttc agcttggcct
    10141 gcgcggcggt gaacccccgc cccaggaact ggcccgccag gtcggagccg gcgtcggcgg
    10201 gcacggggcc tccgccctgg gccttcatcc cgatcacgcc caggccggcg gccccgcatt
    10261 gctccagcgc cgcctgcata tccgccgctt gcagaaggcg gaaattgcag gccgacagga
    10321 ccgcgtccag ccagcccagg gccgccgcct ggcgcaattg ctgcgccatg ttccggtgcg
    10381 tgctgaagcc gaagtaacgg atccggcccg accccttggc ggcctccgcc cagtctcgga
    10441 cgtcgtcggt cagagtctcc acgccgcgca ggtcgtggat cagatagagg tcgatatggt
    10501 ccgtgcgcag cttctccagc gagcgcttca gcgagttgga caggccttcg gggtgatggg
    10561 cgtcggtctt ggtgatgaga aagacgtcgc gccttgcggc gggctgcttt tcgaagaatt
    10621 ggccgaaccc cgcctcgctt cggtcgccgt ggtatccttc cgccgtgtcc cagcacgtga
    10681 cgcccagttg cagcgcgcgg tgcaggatat gctggttggc cggcacgtcg aaaatgccgc
    10741 cgaggcagag cgacgatatg tgaacgcctg tccgtcccag gatccggcgc ggaaccgttc
    10801 cgccgccttc tccggcggcg gacgctggaa gcccggcgga tagaagcgag ccggtgcccg
    10861 ccagcgtcag ggccttcaga aaggatcgcc gagtgatcga atcgcctgcc atcgcggtga
    10921 ctccctaatg ggtggcaccc accgtacccc ccggccggcc gggacggtag ggcgctacag
    10981 cagaaatccg ctgcgggcct cgcggctttc gccgtcggcg gtcacccgga tggcccggac
    11041 ggtcgcgagc ggacacacgc tctcgcacac gccgcacccg atgcaccgct ccggatcgac
    11101 gaccggccgc tggagcagca cgagcagctc cgcgacgctt cccttcggcg gcggcgattc
    11161 ccacgggtgg ttgggatcga tgctccattg ggcgggcccg cactcgatga tccggcgcgg
    11221 ctcggcgtcg aaatccccgg cgatgcggca atagaggtcg tccgatacgc gggccgcggc
    11281 gtcggtctcc ccctcgaaaa acaacgtgag ttcgtccccc cgcgcgattt cacgccggcg
    11341 gccgggcagg ggctcgaaga tctcctggat gctgatggcc ttcgggctga ccgggcagtt
    11401 ctcctcgcat acgatgcagg ggcggttcat ggcccagggc aggcagcggc cccggtcgac
    11461 gaaggccgtg cccatccgca cggggccgcg ctcgcggaag gcgccgcgcc cgatcttctc
    11521 gttgagcgtc agcggccgga tggcgccggt ggggcagatc tggctgcagg ccgcgcaccg
    11581 caactggcag ccgcctattc ccaggctgaa cttcgcgacc ggcgtgccga atccttccag
    11641 gccggcttcg gtgagcgccg ggtggagaat gttggtcggg caaatgcgca tgcactgccc
    11701 gcagcggagg catcgggtca ggaattcgcg ttcgggtccg gcgcccggcg ggcggatccg
    11761 ccgcgggtcg cggccgccgg ccaggcggcc cagcggcgcc acggcgaaac cggccagcag
    11821 ggaggcgatc actccgcgcc gcgtgaggtc cggcgcggcg cgctcgccgg aggcggaacg
    11881 cgccagccgg tagccgacca ggtcatcggg gcaggcatgc aggcagttca tgcacagcac
    11941 gcattcgtgc gtgcggatgc ggcctgccgg ctggcaggcg ccttcgcagg cggcttcgca
    12001 cttcaggcag ttcgagcagt cgcgctgtct cttcccgacg cgccatagcg caaagcggcc
    12061 caggacgccg tacagcgcgc ccagcggaca cacgtagcgg cagtagaaac gggggatcca
    12121 cagattgacc agcagggcgg caagaaacag cccgccgatg agcgcggcgc cctgggccag
    12181 ccgcgcgccg gtccagagga catggattcc gcgatcggcg agcggcagga tcaccaggtt
    12241 caccgcgcga tgcatgaagg aaatcgggtc gagccagccc gtctgcaggc tgccggcggc
    12301 gaactcgcgg atgctctcca gccgcgcgga ttgcccggcc aggcgcccga ccgccggcgc
    12361 gagcaggccg cccgccgccg cggccagcag gaacgcgagc aggtaatact tgatccgttg
    12421 cgcgggccgg aaggcgcggc gcaggcactg ctccgcgggc gcggcgcggc gcaggcccag
    12481 ccagccgatc gcctggtgca gcgtaccgaa cggacagacc cacccgcaga agaaccggcc
    12541 caggatcacc gtcagcgcga tgactagcaa ggaccattcc agacccgctg gcagcgatcc
    12601 cgtgccgagg accacgccga gggccgtcag cggattcagt tgcaggaacc agttgaccgg
    12661 ccacccgcgg agccgccacc actcttcgcc gaccgtcgtg acgaggcaga accacaggaa
    12721 caacagcaga aaaaagacct ggctgatccg ccggacccac gtgatcttca tgaagcccct
    12781 gcctggatgc gtaccgggtt gagcagttcg tagctggccg tccccgcgcc cgccgcggcc
    12841 gcccgcgcca ggtgggggag gtccgacgct tcgcggccga gcagcgtgac gccgaacgcg
    12901 tccaccgcga cgggatccgt gccgacgacc agggtgtccg ttgccttgag gtcggccagc
    12961 gagccgccgg tcgggccgtt gcggatcatc gtcgtcgtcc cgtccaggat gaccagcgtg
    13021 ggccgcacga gctgcgcgag ttcggcgatg agcgtgtgga tgtcctggtg gaacacgctg
    13081 cgccgcccgc cgagcagtcc gtaccagttc ttcagggtca ggcttgcgcc gctgcggtag
    13141 tgatccttga ccggcgtcag cccgatcagc cggcggatgc ccttgaaggg gcccttgagg
    13201 aacggccaat gccggaggag ccgtccgccc ggcagcgtca ccggcgcgaa atcgtcctcg
    13261 cgcggcaccc acagctccgc gccggcgcgc cgcgccgccg cgccaatccc ggaccattca
    13321 aaacaggaag cctggtcgtg gatcggatgg tccgtgacca cgacgcgcga tgcccccgcc
    13381 cgcaggcatt gcgcgacggc ctcggccacg aggtccggat gcgtcgtcgc cccgagcgtc
    13441 gcgggcgagg caaaggccgc gttgaccttc aacagcacgc cgtccccggg cttcacgaag
    13501 cgggcccacc cgcccagggc ttccatggcc ttgcgaagat tcgtcgtgcg gtccggcccg
    13561 gtgacgatcg ccaggcgggg atcggcggcc ggttgcgaga agtccggcac cggccagtcg
    13621 tggacttcct cccggtcggc ccggccggtt ctccagccga gcgcgtggcc cgcgagcccg
    13681 gtcgcggcca gggcggccgc cgccttcagg ccgcgcgcca ggaactcgcg gcgctccgga
    13741 tcgagaggcg gccggctcac ggcgcggcct ccgaaagctg ccggtccagc agcagggcca
    13801 gttgctccgc gctcaagcga tcgccggcct ggtggacgcc cgcgaaatac ggcccgcgcg
    13861 tgaacaggac ctgccaggtg ccgtaaaaat ccaggacccg ggcgtccggc agctccggga
    13921 tcggcggcag cagcatcgcg ccgtcatcca tgatgaactg ctggtaggcc tgggccagtt
    13981 ttgccgcctc cgcctcgctc ccgcgggcgc agacgaatcc ggacaactga aactcgccga
    14041 tctggtaatc cgccacgaac aggtcggtca gcggcgcgaa gcccaacccg tccgtacggt
    14101 agagccgggg atgcccggcg aggcggtgcg cctccggcag ccgcgcgagc tccggcaggt
    14161 ccgcgggctc cgctggcaaa tccgccatga cctgcgccgc cgccttgcgc agcgcctcgc
    14221 gggccgacgc ggcggccgtg cccgcgatga tttccaggta gtacgacccg tgacagaaga
    14281 aaagggcgtt ctccgccagg taggcgtacc gcgtgaaatc ccgcggctcg gcgtcctcgc
    14341 gctgctgctg gctgaacacc gagtacgcgg agcgggggga gcccatgtcg tacaggaacg
    14401 tttccacgac gctgtcctcg tcgccgggga tccccaggcg gcggcactcg agcgatcgaa
    14461 atccggccgg caggtagagt tcggcccggc cgtcgatctt ctcgtagagc gtgtccgggt
    14521 cgaaatattc ggacgggccg agcgccgtca tgccctcggg cgggttcacc cgggagggcc
    14581 gcgcggccgc ggccggacgc ggggcggggg gcgggaggta gtccggcttg tcggcggagt
    14641 acgcggccgg gtgcgggcgg aactgctcga aatagacggc cgtgcccacg ccggccagcg
    14701 cggccaggat ggccaggccc agcagcgttt ccccccagcc ggtttttctc gccatgagtc
    14761 gaatctccgg aacgggcgcc actatacgga aacgcgggcg caggtaaaac agagaatgcg
    14821 ccgcgggatg gaacggcccg gctcgcgcct cgaaagaagg ctcgcccgtt tgacttttca
    14881 tcagtcatgg tgttttgtgc cggcatgaaa aaagcaggct ggctgggctg ggtggcgatc
    14941 gtcctcgcgg gggccgggac cgcggcggcg gcgcccgtgc gcgcacggca cagcgaggcc
    15001 gaactgacgg cggagcaggc cgccatccgg gccggcgaaa ccgtgtggct ggcgctggcc
    15061 atccgcctcg atcccggctg gcacacctac tggctcaatc ccggcgactc cggcatggcg
    15121 ccgaggctcg cgtggagcct gccggaaggt ttttcggcgg gcgcgctggc ggcgccgccc
    15181 ccggcgcgct tcgagtccga ggggctggtc agcctgggct acggggaacg cgtggtcttt
    15241 ctcgttccga tcaccgcccc cgcgggttgg ggcggcgggc cggcgaccgt cggactgaag
    15301 gccacgtggc tcgtttgcag cgacctctgc ctgcccgagt ccgcgacctt ctcgctggag
    15361 ctcaacggcg cggccgccgc ggcgcccgaa gggctgttcg ccgaggcccg ggcgcgcttg
    15421 ccgctcccgc ccgacggctg gacgctccgg gcgttgcgcc gcggccgcga agtgcacgtc
    15481 agcgtggagc cgccagccgg ctccggcgcg gagtcgccca tggtcttttt ccccgcgcgg
    15541 gccgacgaat tcacctatga cacggccacc gtgccgcagc aaggccccgc gctccggctg
    15601 cagatttccc cgctggccga gaccttcccc gaaaaactga tgggcgtgct ggtcgcgcat
    15661 ggcacggctc ttgctctgga tatacccatt gaaaccccgg tcgaagcgga aggagtcgtg
    15721 ccatgaaagg ttgcgcgcgc acggcggtga tcctgatggt cgtagggttg gcgctgatcg
    15781 tgtgggccga gatcagcccg gatggcgccg ccccggactt caccttgccg ggcatcgacg
    15841 gcgtcccgca ctcgctgtcg tcggcccgcg ggaagtacgt cgtgctcgaa tggttcaact
    15901 acgactgccc cttcgtgcgc aagcactacg gcggcgggaa catgcaggcg ctgcagaaaa
    15961 agtacaccga ggcgggcgcg gtctggtttt ccatctgttc ctcggccccc ggcaagcagg
    16021 gccactatcc cgccgaaaaa atggcggaac tggcgaagca gcggaacgtg gcgtccaccg
    16081 ccgtgctgct ggacccggac ggccgggtcg gccggctcta cggggccaag acgacgccgc
    16141 acattttcat aattgatccg gaggggaagc tgatctacca gggcgccgtg gacagcgtgc
    16201 cctcggctga tccggcggac atcaccagcg cggtcaacta cgtccgggcc gcgctcgacg
    16261 ccgcgctcgc gggccagccg gtggaaaaga aagtcaccgc gccgtacggc tgctccgtga
    16321 agtactagcc cggcccgccg gttttccaat gtttggaaaa aacgcggccg aattttccaa
    16381 acattggaaa aatagcttcc ttttttccaa acattggaaa aaaacggccc tgattttcca
    16441 atgcttggaa aaccgctgtc cgcgctcgcc ggtccggact accgggttcc ctcgatcacg
    16501 atcagcgtga tctccggccg cgcgagcagc cgcatcggcg gcccccaggt gcccgcgccg
    16561 cggttgacgt acagccagcg gccgggcgcg acttcgtgca ggccgcgccg cagcgggtag
    16621 atcggtttca ggagccactc gaaggggaac atctggcccc cgtgcgtgtg gccggacagt
    16681 tgcaggtcga accaggcctt ctccgggcgg ctgacgaggg gctggtgctt gagcagcacg
    16741 acgaaccggt ccgccggccg cgccgccgcc agcagacgct cgctgatgaa atagggctgg
    16801 tgcgtgaacg agccggcggg atcgtccacg cccgccagcc atagcccggg cagcggttcc
    16861 gcggcggcgc cgcgcaggag cgtgaagccg gcggcctggt gaaaggccag ggcctcggac
    16921 acgccggcga aatactcgtg gttgccgagc acggcgtagc gtccgcccgg ggcggccagc
    16981 cggcgcagcg gtccggcctt gtcccggagc tgggcggccg gcgcgtcgat caggtcgccg
    17041 gtgctcacga tcaggtccgg cttcagcgcg tggacggcgg agacgacggt atcgaccagc
    17101 cagccgccgc gccgcgccgt gaggtgcagg tcggagatct gcgcgacccg gtatgtcgtg
    17161 ccggccggca ggcggtccga gcgcagcacg atggtttcga cgcgcagcca gcgggcctcc
    17221 gccagtcccc aggccatggc cgcggccgcg acgatccccg cgatcccgac cgttccgcgg
    17281 ggcgagacgc gccggcgcgc cgcccgcgcc gagaagagcg aggcgaggcg gaccagcagg
    17341 ttccacgcat ccagcagcag gccgacggag aagaaccaga acacaatggc gatccaggta
    17401 tagcccgcgg cgccggcgag ttcggccagg agcgggtgcc cgcggttccc gaaccagcgc
    17461 gccaggaacg gcgcggcaaa caggccggtg aagacgatca ggagccaggc cgtgaacagc
    17521 cggagccgcg ggaaggcccg gaccagcttg atccccatgt acgcatgcat cgcggcgtag
    17581 gacagcagca cgatggggaa gatgagcagc atgatcatga cgcgctcacc atacgggcct
    17641 gccgtcacgg gcgcaagcgc cgggcgggcg ctccgcgcgt ggcgcgcccg tcggctcccc
    17701 tttgaatcgc aacaatattc ttcacgggtg tgaagagtat tgttacgcaa taaaacgtaa
    17761 aataaacgtg gaatgggttc ctgcggtttc ctttctatgg gtgagggaag cggcggagcg
    17821 gatacccggg gggggcaatt ggagattaga gatggaggcc tgtttctggt agataagccg
    17881 tgtgaaaagg cggtggtcgg aagcgggcgg ccgttgcctg caggcggcga attcaccgga
    17941 cgcctccctc gggcggcgtc ggaaccagcg cggcgtggcg gatgaatgga agcgaccgcg
    18001 ggcgggatgc gcgtggcgtg ggggcgatcg cgagtacgct gcatgaatca ggacataaac
    18061 gaagccgggg atgaggtgct gcgggccttc attgccctgg agatctctga cgaggtccgg
    18121 gcggcgttgg atcgcctgca gcgggatctc atgaggggcg aggcgcgggt gggatgggtg
    18181 acgccggcgc ggattcatct gaccctggcg tttctcggcg acatctttgc cgcgcagctt
    18241 cccggtctgg cggacgcgct ggatgccgtg gcggaggggt gtccgccgtt cgccttcgac
    18301 gtggccggga cgggcgcgtt cggcccgccg aaatcgccgc gggtggtctg ggccggggtc
    18361 gaggagccga cgggggcgct ggtccggttg caggccgcgg cggccgtggc ggtgacgggg
    18421 ctgggcttcc ggatcgagga gcgcgccttt catccgcacc tgacgttggg ccgggtgcgg
    18481 gaccggcgcg gcgcggccga tttgacttcg cggctggagt cctttagaac tctgcggtgc
    18541 ggcagggtgg aggtccggcg cgttctgctg ctgcaaagcg ttttgagttc ccagggagcc
    18601 gagtaccggg tgcgtcattc gtccgaattg aaaggagcat gagagcatgg cagcgaaatc
    18661 cagtcaggcg gcgagcgcgg agaaaagcat gccggcggcg ttgagcgccg tcctggccca
    18721 gatccagaag gaattcggcg agggcgcgat catgcgcctc ggcaaggagg aggcgcacgt
    18781 ccaggtgccc tcgatctcca cgggcgccct gacgctggac ctggccctcg gcatcggcgg
    18841 cgtgccgcgc gggcgcatca tcgagatctt cggccccgag tcctcgggca agaccacgct
    18901 ggtcctgcac atcatcgcca acgcccagaa gaacggcggc gtagcggcgt tcatcgacgc
    18961 ggaacacgcc ctcgacccgg cctatgcccg caagatcggc gtcaacctcg acgacctgct
    19021 ggtctcccag ccggactccg gcgaggaggc tctgacgatc gccgaccagc tcgtccgcag
    19081 caattccgtg gacgtggtgg tgatcgactc cgttgccgcg ctcgcgccgc gcgcggagct
    19141 cgagggcgcc atgggcgatt cacacgtcgg cctgcaggcc cgcctgatgt cccaggcgat
    19201 gcgcaagctc accggcatcc tgaacaagtc caagacctgc tgcatcttca ccaaccagat
    19261 cc
//
LOCUS       DAOOTK010000051        19139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1977_length_19139_cov_4.996594, whole genome shotgun sequence.
ACCESSION   DAOOTK010000051 DAOOTK010000000
VERSION     DAOOTK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1977_length_19139_cov_4.996594"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK634_07480"
     CDS             complement(<1..889)
                     /locus_tag="PK634_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOW97614.1"
                     /translation="MHFHALADWTGEQSRLPFSIRVLLESVLRRQGHPAFRAEHVRAL
                     ARWTPDATQKEEVPFLPARVLLQDFTGVPCVVDLAALRGAMARAGRDPKAIEPQIPVD
                     LVIDHSVQVDETASPGALEANMRLEFERNAERYEFLRWGQTAFRKLRVVPPGLGICHQ
                     VNLEHLAQVVATETDAEGRVVAFPDTLVGTDSHTTMINGAGVLGWGVGGIEAEAAMLG
                     QPIPVLTPVVTGVRLTGRLAAGVTATELALTVTQLLRKHGVVEQFVEYFGPGLDTLSL
                     ADRATVANMAPEYGATMGFF"
     gene            1287..2030
                     /locus_tag="PK634_07485"
     CDS             1287..2030
                     /locus_tag="PK634_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97615.1"
                     /translation="MIRKALLVLALVSLTAGVSRGESSRLMLSVENKMPALHHLELGA
                     LAQYEDVPAVFPAWSDSEAYLVEPYARFGLFRNFTVFTRVPYAAVEPDSGDSYDGLGD
                     VSAGFELVAFEDYFDFPYIIPHAEVIFDTADEGLGYGDTRLLGGVAIGTVVDDQWHYV
                     LDVGYMSSDDDDDLYRLSGGFVWDLNKQCSLLAEGLVTGEDYEDNSYTLYGQGGISYR
                     PFEELSYVFYAGTGDAVDFSAALKIAYSF"
     gene            2056..2355
                     /locus_tag="PK634_07490"
     CDS             2056..2355
                     /locus_tag="PK634_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOW97616.1"
                     /translation="MNRLTSPYRCHLFICGNDRQGARKSCADGGAGPAIKDILKDQVA
                     ARGWKGPVRVSQSGCLGLCERGPNVMIYPQGLWFSAVTPADADAILAEVEKLLKG"
     gene            complement(2406..2960)
                     /gene="rfbC"
                     /locus_tag="PK634_07495"
     CDS             complement(2406..2960)
                     /gene="rfbC"
                     /locus_tag="PK634_07495"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOW97617.1"
                     /translation="MQCEKTPLPGVLVFQPDRFGDSRGFFMETFHRDKYRPLGLDREF
                     VQDNFSRSGRGTLRGLHYQLRRPQAKLVMVMAGEVFDVAVDIRRGSPHFGKGFGMVLS
                     GDNRRQLFIPEGFAHGFCVVSEQADFLYKCTDFYDPADDRGILWSDPAIGIHWPGKDF
                     LLSSKDGRLPKLADLPESELPVFG"
     gene            complement(2969..3844)
                     /gene="rfbA"
                     /locus_tag="PK634_07500"
     CDS             complement(2969..3844)
                     /gene="rfbA"
                     /locus_tag="PK634_07500"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOW97618.1"
                     /translation="MKGIILAGGSGTRLYPITRAVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVISTPEDLPRFRALLGDGSQIGLRFAYAEQPRPDGLAQAFLIGRAFLAGSPS
                     ALILGDNIFYGHGLQEICRAAGRLTEGGLIFGYPVKDPERYGVVEFDPTGRVLGIEEK
                     PAKPRSRYAVPGLYFYDGSAADVAAELKPSARGELEITDLNLAYLRRGTLRVELLGRG
                     FAWLDTGTHESLQQASNFVQTIEDRQGLKIACIEEIAFRLGYIDAAQLRRLASDMMKN
                     EYGRYLARIAAGTEG"
     gene            complement(3940..4995)
                     /gene="rfbB"
                     /locus_tag="PK634_07505"
     CDS             complement(3940..4995)
                     /gene="rfbB"
                     /locus_tag="PK634_07505"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOW97619.1"
                     /translation="MNNLLVTGGCGFIGANFIRYLLEEAPGFTGRVINVDKLTYAANP
                     LSLADIEKRFPGRYVLVRADIVDRPAMARALDAHGVDAIGHFAAESHVDRSIVRPDDF
                     IQTNVVGTHVLLELFRERRDRIARFHHVSTDEVFGSLGPTGYFTEDTPYRPNSPYSAS
                     KAASDHLARAWHETYGLPITISNCSNNYGPYQFPEKLISLMILNALEGKPLPVYGDGK
                     NVRDWLYVRDHCTAIWRVLTRGAPGRTYNVGGRSERPNLDVVGLVCDLVDELAGPRPG
                     GKPRRELIAFVKDRPGHDRRYAIDCSRIERELGWRPEESFETGLRKTVRWYMEHREWV
                     DQVRTGEYQRWIKAHYG"
     gene            complement(4992..5879)
                     /gene="rfbD"
                     /locus_tag="PK634_07510"
     CDS             complement(4992..5879)
                     /gene="rfbD"
                     /locus_tag="PK634_07510"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOW97620.1"
                     /translation="MRIFVTGSKGQLGRDSLDVFASLAEVAGLDLPDLDIADAGAVER
                     TLSAFKPDAVINAAAYTQVDRAETDREAARRANADGPRVLAEYVERHGGLLVHVSTDY
                     VFDGRRPPPRPYVETDPTGPASVYGRTKLEGEEAVRRATERHLILRTAWLYGASSRGF
                     LRAILGRAVSNPGAPLRVVNDQFGSPTWSWRLARQIRKALEGGARGLYHAAGEGACTW
                     FDFAAEFLRLLNVPARVEPCPATEYPRPAPRPRNSVLENARLKAEGLQVMRPWAEDLA
                     EFARLHGERWLREAREAKA"
     gene            5983..6240
                     /locus_tag="PK634_07515"
     CDS             5983..6240
                     /locus_tag="PK634_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HOW97621.1"
                     /translation="MSTEIEIGTITHYFGHLNVAAIKLTASLAVGDTLRIRGHTSDLT
                     VTVDSIQIEHKSVQKAAQGDNVGITVPGHVREHDKVYKVAP"
     gene            6258..7721
                     /locus_tag="PK634_07520"
     CDS             6258..7721
                     /locus_tag="PK634_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOW97622.1"
                     /translation="MDALDLKLDRLDRSFPPDFTPEQVARGKTLFFKRLALSAHRFYG
                     GKMQTVPRVAAFGFNWFNVWYTPGVSAVSTTIRDDHEASYDLSNRGNLVAVVSDSSRV
                     LGDGDVSPSGGLGVMEGKAFLMKMLGGVDAAALCMDSRGPDGKHDPDAIIDFVRRLQP
                     SFGAVNLEDIAQPNCYRVLDVLRESCEIPVWHDDAQGTGCVTLAGLLNALKVAGKELA
                     SARIVLYGAGASNTTIARLILGAGGDPGRLILFDTQGSLGRHREDIRADARFYRKWEL
                     CERTNPGGVRTVEEAVRGADALIALSKPGPGTVKPGWIRAMGPRAIVFACANPVPEIY
                     PHAAKEAGAFVVATGRGDFPNQVNNSLGFPAILKGALLVRARKITDGMAIAAARSLAQ
                     FAEKKGLSPEHIIPTMEEAGVFPQEAADVAMQAVRDGVAGRALSREEVYRRTEEGIRD
                     ARRLLDLMLEQKLVSRPPPELLRDALEGAVAEVRGAR"
     gene            complement(7671..8564)
                     /locus_tag="PK634_07525"
     CDS             complement(7671..8564)
                     /locus_tag="PK634_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW97623.1"
                     /translation="MSTILVTGASGFLGEALVRRLAHAHRVVGAGFQHARDALDLREP
                     AQVRCLLDAAQPDVAIHAAAYRDPDFCEAHPEEAARLNVEPVRAMAAALPPAARLVLV
                     STDYVFDGEAPPYREDRERRPASVYGRTKAAAEDLALARANSLVVRIPLLIGIEPAHR
                     GPSGFVLQIARQVLDGKPAEADDVLVRFPTWTEDVVAAVEFLLERSAAGVFHVSAPRG
                     ATRYRWTLEVARLLGRNADHLVPLRAPVPRPARRPVNSQLSTEKLRFAGFDRFTDFAA
                     VARQILARASDLGDGALQRIP"
     gene            8681..9985
                     /locus_tag="PK634_07530"
     CDS             8681..9985
                     /locus_tag="PK634_07530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOW97624.1"
                     /translation="MKITIVGTGYVGLVTGACFSEVGHQVLCVDKDERKVNMLRANKM
                     PIYEPGLDEVVARNVAAGRLRFSTSLAEGTEYAEVIFIAVGTPPGVEGQADLSYVEQV
                     VREVAEHMSSYRLLVEKSTVPVNTAEQIKRTVLRYSRKDVAFDVASNPEFLREGTAIE
                     DALQPDRVVVGVESERASQLLRAIYEPIIARGGGVFVEMGIASAELTKHASNSFLAMK
                     ISYINAVSRICELAGADVEQVANGMGLDRRIGRQFLKAGVGYGGSCFPKDVDAFVHLA
                     DQLGYDFDLLKNVRQINEAQRDHVMQKIRHELWVIQDKTVAILGLAFKPCTDDVREAA
                     SLYFVPRLREAGAMLRLWDPVATDNFKALYPDLAYFTDIEACVRDADLVLILTEWSQI
                     ADFGLERLRSLMKCPVVVDGRNMFDPKEMRAKGFAYHCVGRP"
     gene            10082..10261
                     /gene="rpsU"
                     /locus_tag="PK634_07535"
     CDS             10082..10261
                     /gene="rpsU"
                     /locus_tag="PK634_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOW97625.1"
                     /translation="MSEVKVRKGESVEKALRRLKKKMDKEGIMREIRSHRHYEKPSEK
                     RRRKQARARLRALKG"
     gene            10326..11255
                     /locus_tag="PK634_07540"
     CDS             10326..11255
                     /locus_tag="PK634_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOW97626.1"
                     /translation="MIIGLTTRWNAREHTEGERMIEEILALGFDHVELGYDLRLDLVP
                     GVMRMVEQKAVRVWSVHNFCPVPVGAPKGHPELFSLSDRDPRERERAVQHTERTIRFA
                     GEVGARVVVAHAGNVDMTKYSRELLALCAEGRLGTPAYEKTKLKLQITREKKAGPHLE
                     GLRESVKRLLPALEAAGVALALENLPTWEAVPTEVEMERLCREIDSPHLRYWHDIGHG
                     HIRAMLGFINPERWLERLSPWLAGMHIHDVRPPALDHAMPPGGLVDFTRFRDRMKSDM
                     VRILEPGPGAPADTVRGALAYLRGVWEGPGTAT"
     gene            11272..12240
                     /gene="gmd"
                     /locus_tag="PK634_07545"
     CDS             11272..12240
                     /gene="gmd"
                     /locus_tag="PK634_07545"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOW97627.1"
                     /translation="MKKALITGVTGQDGSYLAEWLLGKGYAVHGMVRRSSTETFERIG
                     HIKDRLNLVQADLLDELSLIKALEEARPDEIYNLAAMSFVPTSWSQPVLTGEFTGIGV
                     TRLLEAVRLVCPKARVYQASSSEMFGKVREVPQTELTPFHPRSPYGVAKAYGHFITVN
                     YRESYGLFAVSGILFNHESPRRGKEFVTRKITDGVARIRAGLQDKLPMGNIEAKRDWG
                     FAGDYVRAMWLMLQQPEPDDFVIATGENHSVREFLELAFARAGLHWEDHVVIDSALLR
                     PAEVEHLLGDCAKARAKLGWKPEVDFPGLVRMMVDADLERYGVRSK"
     gene            12242..13180
                     /locus_tag="PK634_07550"
     CDS             12242..13180
                     /locus_tag="PK634_07550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOW97628.1"
                     /translation="MNVLVTGAGGFVGRHVVADLAAAGHAPIGFDRPGVPLPPAASPR
                     SGDILDRAALDALVRELQPGGLIHLAGAAFVPMGWTDPDAVFSANLLGAIRVMEAFRH
                     HAPRARLLMVTSAEVYGRSGAPGRLDEESPLLPDNLYALSKQAVDSAARMAAAHYGMP
                     IVVARPGNHIGPGQSPSFVAAAFAEQVAAIALGRAEPVVRVGNLDSLRDFLDVRDVAR
                     AYRILLERGEPGRAYNISSGKLVRVGDLLEGLCRLAGIRPRVEVDPARYRPAEEPRPL
                     DSGRIRRELGWEPAVPLDGTLRAILDEALRRAGKAG"
     gene            13185..14225
                     /locus_tag="PK634_07555"
     CDS             13185..14225
                     /locus_tag="PK634_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOW97629.1"
                     /translation="MSRARRFLSLGVRLALCIGLMVFLLRRLDLSEFGRVFAGAAGQW
                     PWWLAGLGFTFLGLFAGAWRWGEILAAQGLRISFGQVFSIFFIGQFFNAFMLGACGGD
                     VARAYYVARRAGGRRTEAASTVLVDRAIGLWVTIAFGCLMIASRHALFAHHPASRWPA
                     MLMWILLAGSVLAAWLLFRRNLAEHPWLAARAKPASRLGALARRMYAAFYLYRQRPGL
                     VAGAAALSLVNVFGLTMACWSFARSLGVGVPLLVFFALFPVITVIAAIPLTPGSLGVR
                     ESLFVTLFAAVQVGDETAMSLSLLVYAGSLVWSLFGGAIFLGYAAGEGHGFREELEAI
                     QHDAPAAGEGLP"
     gene            14222..14509
                     /locus_tag="PK634_07560"
     CDS             14222..14509
                     /locus_tag="PK634_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOW97630.1"
                     /translation="MTWAQAAPGGARLVLRIVPRADRNAVQGLHGDALKIRLQAPPVD
                     NKANEALVEFLAGRLSLPRHSIRLVAGASGRNKQVLVTGLEPGEIVRRLTG"
     gene            complement(14499..16214)
                     /locus_tag="PK634_07565"
     CDS             complement(14499..16214)
                     /locus_tag="PK634_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97631.1"
                     /translation="MNAHPPVPTGELEKYSSALTLSDMEIFVFPELLYSLVLANLMSP
                     RLWAWRQDPWFAKLDSLTPYRRVLRLKQFVIDHYEFNLDLDTWGLTTKEREIERFRPW
                     MDEATIAQSNALFGYEGDKYYFSLDIRRHFGLDKYEGHVIPYWKTETLEAMDAFRFKE
                     GYRAGAGECVSLSTLYAAALYAICGIPLEDIFLMGTALHSQNFVDVGDGILTNNRRVV
                     TKAMWFNGTELTAKAQRALRHEQVTIVAHPSGYIHTVYPEATIAPAAWDRFRDRLRGF
                     LATDINMEILANYLRQKPELQSCFQITHDFGGKARHIPAEKVYHYEHTSSFKVSDRTR
                     DKLLEEIEEDEFFAEPLDGRIPLNKFEDFFRRNKIDLTREEDVQKLMKEFTCYNMRAS
                     DIIRDLVGFTRLEPRLPSGEKRFRDVPAIGLRPGMTREHIVAEVEALRGKSRIADLAF
                     YAYRDLSRCDWAPFLKAALERSPVTLKRAEGQDDRQAAAWLEGLPNESIYDGARVAQP
                     DEVANYGRGDGLEKALGLAGIWKNRHPGEPVELDCRPDRVRLSQGARRVEWPSAKGLE
                     KNVTL"
     gene            16332..17729
                     /locus_tag="PK634_07570"
     CDS             16332..17729
                     /locus_tag="PK634_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97632.1"
                     /translation="MSTDEQSAEQPRSAPGDLVLELNFVPTWARQPPAANPYAGFEGE
                     RRREPRDRDRPPRGGPGAGPGRGPGRGPKPRDRDERERRGFPSRRPPREGPVEPAPAP
                     VAVSFLPERRHLGVLAHELRASGRAYPLADLASRFLQTPACHEVKIEARGGSGGQPGS
                     QLVQCRACQRLFQNLSEFTGHALAVHLDTVFVREQQQAEPPAGAFNCVARCRLSGELL
                     GPPNYHAYNARLQELWRTRFAHMTLDEYRSHIETVREPEQIEKWKQSVSVRTAYRFKD
                     QPELPTLSEAEAQKIFAEQHLPKLALTGRRFIIRAELAQQLTDQGLRRAVRESWFRES
                     RHPYSVMMALRPAFHHMHLYLFRTAGGQTFATSIQPHPLEPRRAVKSIAEVLHYLRRH
                     PGCARHELVEKLRPGMAADSPQAAMVLSPLRWLIEKGHVIEFFDGTLSVPGGPPSAHE
                     PHDHAPGGEAPAAHP"
     gene            17726..18772
                     /gene="hisC"
                     /locus_tag="PK634_07575"
     CDS             17726..18772
                     /gene="hisC"
                     /locus_tag="PK634_07575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOW97633.1"
                     /translation="MNRSRIRQAVERMRAYTPGEQPQEPGFIKLNTNENPYPPTPAIR
                     EALERPPDRLRLYPDPLCQELRRELARLHGCRAEQVFVGNGSDEVLALCVRAFVERDG
                     TIGFFDPSYSLYPVLAAAEEIAVRPVPLKPDFTWDMPAGYAASLFFLTTPNAPTALQY
                     PRETVRSFCAGFDGVVVLDEAYVDFAREHFMDMALEFGHVLVARSLSKSYSLAGLRVG
                     YVVGAEPLIAAMYKIKDSYNVDRLAQEIALAAIRDQAHMRANAARIRATRERAAAALR
                     TRGFEVGPSEANFLWARPSRPAARDLYEALRARKILVRYFPGERTGAHLRVTIGTDGE
                     MDALLRALDEIGAG"
     gene            complement(18780..>19139)
                     /locus_tag="PK634_07580"
     CDS             complement(18780..>19139)
                     /locus_tag="PK634_07580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOW97634.1"
                     /translation="VLVLTMPLWNCGMPAIMKAWLDQVLVPGVVFKYEAGSIIPLHHI
                     TRAILLASSSDIVREGDPQDQLTPQLTAILNSVGIQNFSIAWADGQHPSHLDAEDRKA
                     TAIEAAQELADEVVEEA"
BASE COUNT         2864 a         6543 c         6723 g         3009 t
ORIGIN      
        1 ggaagaatcc catcgtcgcg ccgtactccg gcgccatgtt cgccaccgtc gcgcggtcgg
       61 cgagggacag ggtgtccagg cccgggccga agtactccac gaactgctcg acgacgccgt
      121 gcttccgcag cagttgggtg acggtcagcg cgagttccgt cgccgtgacg cccgccgcga
      181 gccggccggt gagtcgcacg ccggtgacga cgggcgtgag cacggggatg ggctggccga
      241 gcatggcggc ctcggcctcg atcccgccga cgccccaccc gagcaccccg gcgccgttga
      301 tcatcgtcgt gtgcgagtcc gtgccgacga gcgtgtcggg gaacgcgacc acccggccct
      361 cggcgtcggt ctccgtggcg acgacctgcg cgagatgctc caggttgacc tggtggcaga
      421 tgcccaggcc gggcggcacg acccgtagct tgcggaaggc ggtctggccc cagcgcagga
      481 actcgtagcg ctccgcgttg cgctcgaact cgaggcgcat gttcgcctcg agcgcgcccg
      541 gcgaggcggt ctcgtccact tggacggagt ggtcgatcac gagatccacg gggatctggg
      601 gctcgatggc cttcgggtcg cggccggcgc gggccatggc gccgcgcagc gcggccaggt
      661 ccacgacgca gggcacgccc gtgaaatcct gcagcagcac gcgggcgggc aggaagggga
      721 cctcttcctt ctgcgtggca tccggcgtcc atcgggcgag ggctcggacg tgctccgccc
      781 ggaaggccgg atggccttgt cgccgcagaa ccgattcgag caggacgcgg atggagaagg
      841 ggaggcggct ctgttcgccg gtccagtcgg ccagggcgtg gaaatgcatc gtccgaccgt
      901 tcttcagggt gagatggtcg agcgttttct tctcgtggtt catgatgggt tcccggttga
      961 aaacggggcc gaccttaagg ccaaccgcgg ggcaaagtca accggcgccg aaccgcccgc
     1021 atcccggttc ggcggatgcg taacaaatcg cttcacacgt gtgaatatta ttgttacgca
     1081 ttcaatacgg ggaaggcggt gcggctgcat cggccggtcg cttcgcggcg gccgcgcccg
     1141 gcggtcggcg cggcccggcg ggggcctccg cgcgccggag aacccgcgaa cctctttttt
     1201 ttgtagcatt tgaggggaag gctcattatg gtgtcgggaa atacaggagc ggccataaag
     1261 caacctgcac cacggggaga aacatcatga tccgcaaagc actgcttgtc ctcgccttag
     1321 tgtccctgac cgccggcgtg agccgcggcg aatccagccg gctgatgctg tcggtcgaaa
     1381 acaagatgcc cgccctgcac cacctggaac tcggggccct ggcccagtac gaggacgtgc
     1441 cggccgtctt ccccgcctgg agcgattcgg aagcctacct ggtggagccg tacgcgcgct
     1501 tcggcctgtt ccgcaatttt accgtcttca ctcgcgtccc gtacgcggcc gtcgagccgg
     1561 attccgggga ttcatacgat gggctgggcg atgtctcggc cggtttcgaa ctcgtggcct
     1621 tcgaggacta cttcgacttc ccctacatca ttcctcatgc ggaagtcatc ttcgacacgg
     1681 ccgacgaagg cctggggtat ggcgacaccc gcctgctggg cggcgtggcg atcggcaccg
     1741 tggtggatga ccagtggcat tatgtgctcg acgtcggtta catgtcctcc gatgacgatg
     1801 acgacctgta ccggctgtcc ggcggcttcg tctgggacct gaacaagcag tgctcgctcc
     1861 tcgcggaagg cctcgtcacg ggcgaggact acgaggacaa cagctacacg ctctacgggc
     1921 agggcggcat cagctaccgt ccgttcgagg aactgtccta cgtcttctac gcgggcacgg
     1981 gcgacgcggt cgatttctcc gccgcgctga agatcgcgta cagtttctaa cctccgattc
     2041 gcgggagggc ggggcatgaa ccggctgacg tctccctacc ggtgccacct gttcatttgc
     2101 ggcaacgacc gccagggcgc gcgcaagtcg tgcgccgacg gcggggcggg cccggcgatc
     2161 aaggatatcc tcaaggacca ggtcgccgcc cgcggctgga aggggccggt gcgcgtgtcg
     2221 cagagcggct gcctcggcct gtgcgagcgc ggccccaacg tgatgatcta tccccagggc
     2281 ctctggttca gcgccgtcac gcccgccgac gcggacgcca tcctggccga ggtcgaaaag
     2341 ctgctcaagg ggtagggcgc gttgaatcca cgcgccggac agacggcggc ttgagccaag
     2401 ccgccctacc cgaacacggg caattccgac tccggcaggt ccgccagctt cggcaggcgc
     2461 ccgtccttcg acgacagcag gaaatccttc cccggccagt gtatgccgat cgccgggtcc
     2521 gaccacagga tcccgcggtc gtcggcgggg tcgtaaaagt ccgtgcactt gtacaggaag
     2581 tccgcctgct cgctcaccac gcagaagccg tgcgcgaagc cctcggggat gaacagctgg
     2641 cggcggttgt cgccggagag caccatgccg aatcctttcc cgaagtgcgg gctgccgcgc
     2701 cggatatcca ccgccacgtc gaagacctcg ccggccatca ccatcaccag cttggcctgc
     2761 ggccggcgca gctggtagtg caggccccgc agcgtgccgc ggccggagcg cgagaagttg
     2821 tcctgcacga actcgcggtc caggccgagc gggcggtact tgtcgcggtg gaacgtctcc
     2881 atgaagaagc cgcgcgagtc gccgaaccgg tccggctgga agacgagcac gccgggcagc
     2941 ggggtctttt cgcattgcag ggacatggtc agccttccgt ccccgcggcg atgcgcgcca
     3001 ggtagcggcc gtactcgttc ttcatcatgt ccgacgccag ccgccggagc tgggcggcgt
     3061 cgatgtagcc gagccggaag gcgatctcct cgatgcaggc gatcttgagc ccctgccggt
     3121 cctcgatggt ctgcacgaag ttcgaggcct gctgcagcga ctcgtgcgtg ccggtgtcca
     3181 gccacgcgaa cccgcgtccg agcagctcca cgcgcagcgt cccgcggcgg aggtacgcga
     3241 ggttgaggtc cgtgatctcc agctcgccgc gggccgacgg cttgagctcc gccgcgacgt
     3301 ccgccgccga gccgtcgtaa aagtatagcc ccggcacggc gtaccgggac ctgggtttcg
     3361 ccggcttctc ctcgatgccg agcacccggc ccgtcgggtc gaactccacc acgccgtatc
     3421 gctcggggtc cttgacgggg tagccgaaga tcagcccccc ctccgtcagc cggccggcgg
     3481 cgcggcagat ttcctgcagg ccgtggccgt agaagatgtt gtcgcccagg atcagcgccg
     3541 acgggctgcc ggcgaggaac gcgcgcccga tgaggaacgc ctgggccagg ccgtcgggcc
     3601 ggggctgctc ggcgtacgcg aagcgcaggc cgatctgcga gccgtcgccc agcagcgccc
     3661 ggaagcgggg caggtcctcg ggggtggaga tgaccaggat gtcccggatc cccgcgagca
     3721 tcagcacgga gagcgggtag tagatcatcg gcttgtcgta gaccggcagg agctgcttgc
     3781 tcaccgcccg cgtgatcggg tagagccgcg tccccgagcc gcccgccagg atgatgcctt
     3841 tcatgccgtg ctcccggttt tccaatcatt ggaaaaacca cgcccatttt ttccaatcat
     3901 tggaaaaaaa gcgagccgag ttttccaaac attggaaaat cagccgtagt gcgccttgat
     3961 ccaccgctgg tactcgccgg tgcgcacctg gtccacccac tcgcggtgct ccatgtacca
     4021 ccggacggtc ttgcgcaggc cggtctcgaa cgactcctcc ggccgccagc ccagctcgcg
     4081 ctcgatccgc gagcagtcga tcgcgtagcg ccggtcgtgg cccggccggt ccttgacgaa
     4141 ggcgatcagc tcgcgccgcg gcttcccgcc cggccgcggc ccggccagct cgtccaccag
     4201 gtcgcagacc aggccgacga cgtcgaggtt cggcctctcg ctccggccgc cgacgttgta
     4261 ggtgcggccc ggcgcgcccc gcgtcagcac gcgccagatc gcggtgcagt gatcgcggac
     4321 atagagccag tcgcggacgt tctttccgtc gccgtagacg gggagcggct tgccctcgag
     4381 ggcgttgagg atcatcaggg agatcagctt ctcggggaac tggtacggcc cgtagttgtt
     4441 cgagcaattg gagatcgtga tcggcaggcc gtaggtctcg tgccaggcgc gggcgaggtg
     4501 gtcggacgcc gccttggacg cggagtaggg gctgttgggc cggtacggcg tgtcctcggt
     4561 gaagtatccg gtcgggccga gcgagccgaa gacctcgtcc gtgctgacgt ggtggaagcg
     4621 cgcgatccgg tcccgccgct cgcggaacag ctcgagcagg acgtgcgtgc cgacgacgtt
     4681 ggtctggatg aaatcgtcgg gccggacgat ggagcggtcc acgtgcgact cggcggcgaa
     4741 gtggccgatc gcgtccacgc cgtgcgcgtc cagcgcgcgg gccatggccg ggcggtcgac
     4801 gatgtcggcg cgcacgagga catagcggcc ggggaaccgc ttctcgatgt ccgccaggct
     4861 caaggggttg gcggcgtagg tgagcttgtc cacgttgatc acgcggccgg tgaagccggg
     4921 ggcctcctcg agcaggtagc ggatgaagtt ggcgccgatg aagccgcagc cgccggtgac
     4981 gagcagattg ttcatgcctt cgcctccctc gcttcccgga gccatcgctc gccgtggagc
     5041 cgcgcgaatt ccgccaggtc ctccgcccac ggccgcatga cctgcaggcc ctcggccttc
     5101 agccgcgcgt tctccaggac cgagttgcgc gggcgcggcg ccgggcgcgg atactcggtc
     5161 gccgggcacg gctccacgcg ggccggcacg ttcaggagcc ggaggaactc cgcggcgaaa
     5221 tcgaaccacg tgcaggcccc ttcgccggcg gcatgataca ggccgcgggc gccgccttcc
     5281 agcgccttcc ggatctgccg ggccaggcgc caggaccacg tgggcgagcc gaactggtcg
     5341 ttgacgacgc gcaggggggc gccgggattc gacaccgcgc ggccgaggat ggcgcgcagg
     5401 aagccgcggc tgctcgcgcc gtagagccag gccgtgcgca ggatgaggtg ccgctccgtg
     5461 gcccgccgca ccgcctcctc gccctcgagc ttggtgcgcc cgtacacgga ggccgggccg
     5521 gtcggatcgg tctcgacgta ggggcggggc ggggggcgcc ggccgtcgaa gacgtagtcc
     5581 gtcgagacgt ggacgagcag cccgccgtgg cgctcgacgt attccgccag cacgcgcggg
     5641 ccgtccgcgt tggcgcgccg ggcggcctcc cggtcggtct cggcccggtc cacctgcgtg
     5701 taggccgcgg cgttgatcac cgcgtcgggc ttgaaggcgg agagcgtccg ctccaccgcg
     5761 cccgcgtcgg cgatgtcgag gtccggcagg tccaggccgg ccacctccgc gagggaggcg
     5821 aagacgtcca ggctgtcccg gcccagctgg cccttcgatc ccgtgacgaa tattctcatg
     5881 cggggcgcca gtctacgcga ggcgcgcggc aaaagccagc ggaagaactt gattcgggcc
     5941 gcgcgcgtgc tagcgtggtg gcgtgcacac gggaggtgct ccatgtcaac ggaaattgaa
     6001 atcggaacga tcacgcatta cttcggtcac ttgaacgtgg cggcgatcaa gctgacggcc
     6061 tcgctggcgg tcggcgacac actgcgcatc aggggccaca cgtccgacct gacggtgacg
     6121 gtggactcca tccagatcga gcacaagtcc gtgcagaagg cggcgcaggg cgacaacgtc
     6181 ggcatcacgg tgccgggcca cgtgcgcgag cacgacaagg tctacaaggt cgcgccctga
     6241 ccggccggat ggaacggatg gatgccctgg acctgaaact ggaccggctc gaccggtcct
     6301 tcccccccga tttcaccccc gagcaggtgg cccggggcaa gacgcttttc ttcaagcggc
     6361 tcgccctctc cgcgcaccgc ttctacggcg gcaagatgca gaccgtcccc cgggtcgccg
     6421 ccttcggctt caactggttc aacgtctggt acacccccgg cgtgtcggcc gtctcgacga
     6481 ccatccgcga cgaccacgag gcctcctacg atctctccaa ccgcggcaac ctggtcgccg
     6541 tggtcagcga ctcctcgcgc gtgctggggg acggcgacgt gtccccgtcc ggcggcctgg
     6601 gcgtgatgga gggcaaggcc ttcctgatga agatgctcgg cggcgtcgac gccgcggccc
     6661 tctgcatgga cagccgcggc ccggacggga agcacgaccc cgacgccatc atcgacttcg
     6721 tccgccgcct gcagccctca ttcggcgcgg tcaacctcga ggacatcgcc cagcccaatt
     6781 gctaccgcgt gctcgacgtc ctgcgcgagt cgtgcgagat ccccgtctgg cacgacgacg
     6841 cccagggcac cggctgcgtc acgctcgccg gcctgctcaa cgccctgaag gtggcgggca
     6901 aggagctggc gtcggcccgg atcgtcctct acggggcggg cgcatcgaac accaccatcg
     6961 cccggctgat cctcggcgcg ggcggcgatc ccggccggct gatcctgttc gacacgcagg
     7021 gctcgctcgg ccggcaccgc gaggacatcc gcgccgacgc gcggttttac cgaaagtggg
     7081 agctgtgcga gcggaccaac ccgggcggcg tgcggaccgt ggaggaggcc gtgcgggggg
     7141 cggacgcgct gatcgcgctg tccaagccgg ggcccggcac ggtcaagccg ggctggatcc
     7201 gcgccatggg cccgcgcgcg atcgtcttcg cctgcgcgaa ccccgtgccg gagatctacc
     7261 cgcatgccgc gaaggaggcc ggcgccttcg tcgtggccac cgggcgcggc gacttcccga
     7321 accaggtgaa caactcgctg ggcttccccg ccatcctcaa gggcgcgctg ctggtccgcg
     7381 cgcgcaagat caccgacggc atggccatcg ccgcggcgcg ctcgctggcg cagttcgcgg
     7441 aaaagaaggg cctttcgccg gagcacatca tcccgacgat ggaggaggcc ggggtcttcc
     7501 cgcaggaggc cgccgatgtc gccatgcagg ccgtccgcga cggcgtggcc ggccgggcgc
     7561 tctcccgcga ggaagtgtac cgtcgcacgg aggagggcat ccgggacgcc cgccggctgc
     7621 tcgacctgat gctggagcag aaactggttt ctcgtccgcc gcccgagttg ctacgggatg
     7681 cgttggaggg cgccgtcgcc gaggtccgag gcgcgcgcta aaatctgccg ggcgaccgcc
     7741 gcaaaatcgg tgaaccggtc gaaacccgcg aatcgcagtt tctccgtcga caactgggaa
     7801 ttcaccggcc ggcgggccgg cctcgggacg ggcgcccgga gcgggaccag gtgatcggcg
     7861 ttccggccca gcagccgcgc gacctccagc gtccagcgat agcgcgtggc gccccgcggc
     7921 gcgctgacgt ggaaaacgcc ggcggcgctt cgttccagca ggaactcgac ggccgccacc
     7981 acgtcctcgg tccacgtggg gaaccgcacc agcacgtcgt ccgcttccgc cggcttgccg
     8041 tccaggacct gccgggctat ctgcagcacg aagcccgacg ggccgcggtg cgccggctcg
     8101 atcccgatca gcagcgggat gcggacgacc aggctgttcg cgcgcgcaag ggcgaggtcc
     8161 tcggccgccg ccttggtgcg gccgtagacg ctggcgggcc gccgctcgcg gtcctcccgg
     8221 tagggcggcg cctccccgtc gaagacatag tccgtgctga ccaggaccag gcgcgccgcc
     8281 ggcggcaggg cggcggccat cgcgcgcacc ggttcgacat taaggcgcgc ggcctcctcc
     8341 gggtgcgcct cgcagaagtc ggggtcgcga taggcggcgg catggatcgc cacgtcgggc
     8401 tgcgccgcgt ccagcaggca gcggacctgc gcgggctcgc gcaggtccag cgcgtcgcgc
     8461 gcgtgctgga agcccgcgcc cacgacccgg tgggcgtggg ccagccggcg gacgagggct
     8521 tcgcccagga acccgctggc gccggtgacc aggatggtgc tcatggccgt tcccgaccgt
     8581 aacagatggc cccgcgtccg tgaatcgatt tcgcggccgg ggttgcattc tccgcgcgcg
     8641 acgggtagga tgcgccgatt tcctgatgga gcgaacgggc atgaagatca ccattgtggg
     8701 cacgggatat gtcgggctgg tcacgggcgc ctgcttttcc gaggtcggcc accaggtcct
     8761 gtgcgtcgac aaggacgagc gcaaggtcaa catgctccgc gccaacaaga tgcccatcta
     8821 cgaacccggc ctcgacgagg tcgtggcgcg caacgtggcc gcgggccgcc tgcgcttctc
     8881 cacgtccctg gccgagggga ccgagtatgc ggaggtgatc ttcatcgccg tcggcacgcc
     8941 gcccggcgtc gaggggcagg cggatctttc ctacgtcgag caggtcgtcc gcgaggtggc
     9001 cgagcacatg tcctcctacc ggctcctcgt ggagaagagc acggtcccgg tgaacacggc
     9061 ggagcagatc aagcgcacgg tcctgcgcta ctcgcgcaag gacgtcgcgt tcgatgtggc
     9121 ctccaacccg gaattcctcc gggaaggcac ggccatcgag gacgccctcc agccggaccg
     9181 cgtggtcgtc ggcgtcgaga gcgagcgcgc gtcgcagctg ctccgggcca tctacgagcc
     9241 gatcatcgcc cgcggcggcg gggtgttcgt cgagatgggc atcgccagcg ccgagctgac
     9301 gaagcacgcg tccaactcct tcctcgcgat gaagatctcc tacatcaacg cggtcagccg
     9361 catctgcgag ctggccggcg cggacgtgga gcaggtcgcc aacggcatgg ggctcgatcg
     9421 ccggatcggc cgccagttcc tcaaggccgg ggtggggtac ggcggctcgt gcttccccaa
     9481 ggacgtggac gcgttcgtgc acctggccga ccagctcggg tacgatttcg acctgctcaa
     9541 gaacgtgcgg cagatcaacg aggcccagcg cgaccacgtg atgcagaaga tccggcacga
     9601 gctctgggtc atccaggaca agacggtcgc catcctgggc ctggcgttca agccctgcac
     9661 cgacgacgtg cgcgaggcgg cctcgctcta cttcgtcccg cggctccgcg aggccggcgc
     9721 gatgctgcgc ctgtgggacc cggtggccac ggacaacttc aaggcgctct acccggacct
     9781 ggcctatttc acggacatcg aggcctgcgt gagggacgcg gacctggtgc tgatcctgac
     9841 cgagtggtcg cagatcgccg atttcgggct ggagcggctc cggtccctga tgaagtgccc
     9901 cgtggtggtg gacgggcgca acatgtttga tccgaaagag atgcgtgcca aggggttcgc
     9961 ctaccactgc gtgggccgcc cgtgagttgc cgtttgacaa accccgggag ttggcttaca
    10021 cgtgcccggg atcatcagcc gtagaaacac atcgagtggc ggaagggggg aaatacaccc
    10081 agtgtcagaa gttaaagtca gaaaaggcga gtcggtggaa aaggcgctgc gccggctcaa
    10141 gaagaagatg gacaaggaag ggatcatgcg ggagatccgc tcgcaccggc attacgaaaa
    10201 gccgagcgag aagcggcgcc gcaagcaggc gcgggcgcgc ctgcgggccc tgaagggctg
    10261 attccttcgg ttcgcgcgaa aactctcctc cacccggggg agagtttttg ccgttacggg
    10321 gcgtgatgat tatcggcctg accacgcggt ggaatgcccg ggaacacacc gagggcgaga
    10381 ggatgatcga ggagatcctc gccctcgggt tcgaccacgt ggagctgggg tacgacctcc
    10441 gcctcgacct cgtgcccggc gtgatgcgga tggtggagca gaaggccgtc cgcgtctgga
    10501 gcgtgcataa tttctgcccc gttcccgtgg gcgccccgaa ggggcacccg gagctgttca
    10561 gcctgtccga ccgcgatccc cgggaacgcg agcgggcggt gcagcacacg gagcggacga
    10621 tccggttcgc gggggaggtc ggcgcgcggg tggtcgtggc gcacgcgggc aacgtggaca
    10681 tgacgaagta cagccgcgag ctgctggccc tctgcgcgga gggacggctg ggcacgccgg
    10741 cctacgagaa gaccaagctc aagctccaga tcacccgcga aaagaaggcg gggccccacc
    10801 tggagggact gcgggagagc gtcaagcggc tgctgcccgc cctcgaggcg gccggcgtgg
    10861 cgctggcgct ggaaaacctg ccgacgtggg aggccgtgcc gaccgaagtg gagatggagc
    10921 ggctctgccg ggagatcgac agcccgcacc tgcgctactg gcacgacatc gggcacgggc
    10981 acatccgggc gatgctcggg ttcatcaatc ccgagcgctg gctggagcgc ctgtcgccct
    11041 ggctggcggg catgcacatc cacgacgtcc ggccgccggc gctcgaccac gccatgccgc
    11101 cgggcggact ggtggatttt acgcgcttcc gtgatcggat gaaatcggat atggtgcgca
    11161 ttctggagcc ggggccgggc gcgcccgcgg acacggtccg gggggcgctc gcctatctcc
    11221 ggggggtctg ggaaggcccc ggaaccgcaa cgtaagcagg ggaagaaagg catgaaaaaa
    11281 gcgctgatca ccggggtcac gggccaggac gggtcgtacc tggcggaatg gctgctcggc
    11341 aagggctacg ccgtccacgg catggtgcgc cgctccagca cggagacgtt cgagcgcatc
    11401 ggccacatca aggaccggct gaacctggtc caggccgacc tgctcgacga gctttcgctg
    11461 atcaaggccc tggaggaggc gcggcccgac gagatctaca acctcgcggc catgtccttc
    11521 gtgccgacct cctggagcca gccggtgctg acgggcgaat tcaccggcat cggcgtcacc
    11581 cgcctgctcg aggccgtccg cctcgtgtgc ccgaaggcgc gcgtgtacca ggcttccagc
    11641 agcgagatgt tcggcaaggt gcgcgaggtc ccgcagaccg agctgacgcc cttccacccc
    11701 cgcagcccgt acggcgtggc caaggcgtac ggccatttca tcaccgtcaa ctaccgcgaa
    11761 agctacggcc tcttcgcggt ctccggcatc ctcttcaacc acgagagccc ccgccgcggc
    11821 aaggagttcg tcacccgcaa gatcaccgac ggcgtggcgc gcatccgggc cggcctccag
    11881 gacaagctgc cgatgggcaa catcgaggcc aagcgcgact ggggcttcgc cggcgactat
    11941 gtccgggcca tgtggctgat gctccagcag cccgagcccg acgacttcgt catcgccacg
    12001 ggcgaaaacc attccgtccg cgagttcctc gagctcgcct tcgcccgcgc cggcctccac
    12061 tgggaggatc acgtcgtgat cgattccgcc ctgctgcgcc ccgccgaggt ggagcacctg
    12121 ctcggcgact gcgccaaggc gcgcgcgaag ctgggctgga agcccgaggt cgatttcccc
    12181 ggcctcgtgc ggatgatggt ggacgccgac ctcgaacggt acggggtccg gagcaagtaa
    12241 cgtgaacgtg ctggtcaccg gcgccggggg gttcgtcggc cgccacgtgg tggccgacct
    12301 ggccgccgcc gggcacgcgc cgatcggctt tgaccggccg ggggttccgc tgccgcccgc
    12361 cgcgagcccg cgctccggcg acatcctgga ccgggcggcc ctggacgccc tcgtgcggga
    12421 actccagccc ggcggcctca ttcacctggc cggcgcggcg ttcgtgccga tgggctggac
    12481 cgatcccgat gccgtgttct cggccaacct cctgggcgcg atccgcgtga tggaggcctt
    12541 ccggcatcat gccccccgcg cccgcctgct catggtgacc tccgccgagg tgtacggccg
    12601 gtccggcgcc cccgggcgcc tggacgagga gtcgccgctg ctcccggaca acctgtacgc
    12661 cctgtccaag caggccgtgg attccgcggc gcggatggcc gcggcgcatt acggcatgcc
    12721 gatcgtcgtc gcgcgccccg gcaaccatat cggccccggg cagtcgccga gtttcgtcgc
    12781 ggccgccttc gcggagcagg tggccgccat cgcgctgggc cgcgcggaac ccgtggtgcg
    12841 cgtgggcaac ctcgacagcc tccgcgattt cctggacgtc cgcgacgtcg cccgcgccta
    12901 ccgtatcctg ctggagcggg gggaaccggg ccgggcctac aacatttcgt ccgggaagct
    12961 ggtccgcgtc ggcgatcttc tggaagggct ctgccggctg gccggcatcc ggccgcgcgt
    13021 cgaggtcgac ccggcgcgct accgcccggc cgaggagccg cggcctctcg acagcggccg
    13081 gatccgccgc gagctcggtt gggagccggc cgtcccgctc gacgggacgc tgcgggccat
    13141 cctggacgag gccctccggc gcgccgggaa ggcggggtga gcccgtgagc cgcgcccggc
    13201 gtttcctttc cctgggcgtt cgcctggcgt tgtgcatcgg gctgatggtt ttcctgctcc
    13261 ggcggctgga cctttcggaa ttcggccgcg tcttcgccgg cgccgccggg cagtggccct
    13321 ggtggctggc gggcctcggc ttcaccttcc tcggcctttt cgccggcgcc tggcgctggg
    13381 gcgagatcct ggccgcgcag gggctgcgga tctccttcgg acaggtcttc agcatcttct
    13441 tcatcggcca gttcttcaac gccttcatgc ttggcgcgtg cggcggcgac gtggcgcggg
    13501 cctactacgt ggcccgacgc gcgggcgggc ggcggaccga ggcggcctcg accgtgctcg
    13561 tggaccgggc gatcgggctg tgggtgacga tcgccttcgg ctgcctgatg atcgcctcgc
    13621 ggcacgccct gttcgcgcac catcccgcga gccgatggcc cgcgatgctg atgtggattc
    13681 tcctggcggg ttccgtcctg gccgcgtggc tgcttttccg ccgcaacctg gccgagcatc
    13741 cgtggctggc cgcccgggcg aagccggcgt cccgcctggg cgcgctggcc cggcggatgt
    13801 acgcggcgtt ctacctgtac cggcagcggc ccggcctggt ggccggcgcg gccgccctgt
    13861 cgctggtcaa cgtgttcggc ctgacgatgg cctgctggag tttcgcgcgc agcctgggcg
    13921 tgggcgtccc gctgctcgtc tttttcgcgt tgttccccgt catcaccgtg atcgccgcga
    13981 ttcccctgac gcccggcagc ctcggcgtgc gcgaaagcct cttcgtgacg cttttcgccg
    14041 ctgtgcaggt cggcgacgaa accgcgatgt ccctctcgct cctggtctat gccggcagcc
    14101 tggtctggag cctgttcggc ggcgcgatct tcctgggcta cgccgccggg gaaggccatg
    14161 gcttccgcga ggagctggag gccatccagc acgacgcgcc ggcggcgggc gaggggttgc
    14221 cgtgacctgg gcgcaagcgg cgcccggcgg cgcgcggctc gtgctgcgca tcgtcccgcg
    14281 ggccgaccgc aatgccgttc agggccttca cggcgatgcc ctgaaaatcc ggctccaggc
    14341 gccgccggtg gacaacaagg ccaacgaggc gctggtggaa ttcctggccg gccggttgag
    14401 cctgccgcgg cacagcatcc gcctcgtcgc gggcgcttcc gggcgcaaca aacaggtcct
    14461 cgtcaccggg ctcgagccgg gcgaaatcgt ccgccggctt acagggtgac gtttttttcc
    14521 aggccttttg ccgacggcca ttccacgcgc cgcgcgccct gcgacagccg gacgcggtcg
    14581 ggccggcagt ccagctcgac cggttccccg ggatggcgat tcttccatat cccggcgagg
    14641 cccagcgcct tttccagccc gtcgccccgc ccgtagttgg cgacctcgtc gggctgcgcg
    14701 acgcgggccc cgtcgtagat ggattcgttc ggcaggccct ccagccacgc ggcggcctgc
    14761 cggtcgtcct ggccttccgc ccgtttcagc gtgacgggac tgcgctccag cgccgccttg
    14821 aggaacggcg cccagtcgca ccgcgacagg tcccggtagg catagaacgc gaggtcggcg
    14881 atccgcgatt tcccgcgcag ggcttcgacc tccgccacga tatgctcccg cgtcatgccc
    14941 gggcggaggc cgatggccgg gacgtcccgg aaccgttttt ccccggacgg caggcgcggt
    15001 tcgaggcgcg tgaacccgac caggtcgcgg atgatgtcgc tcgcgcgcat gttgtagcac
    15061 gtgaattcct tcatgagctt ctgcacgtcc tcctcgcgcg tcaggtcgat cttgttccgc
    15121 cggaagaagt cctcgaactt gttgagcgga atccggccgt ccagcggctc cgcgaagaac
    15181 tcgtcctcct cgatctcctc cagcagcttg tcccgcgtgc ggtcgctgac cttgaacgag
    15241 ctcgtgtgct cgtagtggta gaccttctcc gccgggatgt gccgcgcctt gccgccgaaa
    15301 tcgtgggtta tctggaagca cgactgcagt tccggcttct ggcgcaggta gttggccagg
    15361 atctccatgt tgatgtccgt ggccaggaag ccccgcagcc tgtcgcggaa ccggtcccac
    15421 gccgccggcg cgatcgtggc ctcggggtac acggtatgga tgtacccgct ggggtgggcg
    15481 acgattgtca cctgttcgtg gcggagcgcg cgctgggcct tggcggtgag ctcggtgccg
    15541 ttgaaccaca tggccttggt caccacccgc cggttgttgg tgaggatgcc gtcgccgacg
    15601 tccacgaagt tctgcgagtg cagcgcggtg cccatcagga agatgtcctc cagcgggatg
    15661 ccgcagatcg cgtagagggc ggcggcgtag agcgtggaca gcgacacgca ttcgccggcg
    15721 cccgcgcggt agccttcctt gaagcggaag gcgtccatgg cctcgagggt ctccgtcttc
    15781 cagtagggga tgacgtggcc ctcgtacttg tccaggccga agtgccggcg gatgtcgagg
    15841 gagaagtagt acttgtcgcc ctcgtagccg aagagggcgt tactctgcgc gatggtcgcc
    15901 tcgtccatcc acggccggaa acgctcgatc tcgcgctcct tcgtggtcag cccccacgtg
    15961 tcgaggtcca ggttgaactc gtagtggtcg atgacgaact gcttgagccg caggacgcgg
    16021 cggtacggcg tgagcgagtc gagcttcgcg aaccagggat cctgccgcca ggcccagagg
    16081 cgcggcgaca tgaggttggc gagcaccagg ctgtagagca gctcggggaa cacgaaaatc
    16141 tccatgtccg acagggtcag cgcggacgag tacttctcca gttcgccggt cgggacgggc
    16201 ggatgagcat tcatgccctc accgtagcac ggcggcggcg gacaatcaaa gcaaggggcg
    16261 cggatgcatt tgctttccct cggggcggcc atctgctaga acgtcggggg tcgcatggcg
    16321 gcgagaacac gatgagcacc gacgaacagt ccgcggagca accccggtcc gcgcccgggg
    16381 acctcgtcct ggaattgaat ttcgttccga cgtgggcccg ccagcccccg gcggccaatc
    16441 cctacgcggg cttcgaaggc gagcggcgtc gggagccgcg cgatcgcgat cgcccgcctc
    16501 gcggcgggcc gggagcaggg ccgggccggg ggccggggcg cggcccgaaa ccgcgcgatc
    16561 gcgacgagcg ggagcggcgc ggctttccct cccggcgccc gccgcgcgag gggcccgtcg
    16621 aacccgcgcc ggcgcccgtg gcggtcagct tcctgccgga gcgcaggcac ctgggggttc
    16681 tggcgcatga actccgcgcc tccggccggg cctatcccct ggccgacctg gcgtcccgct
    16741 tcctgcaaac gccggcctgc cacgaggtga agatcgaggc gcggggtggt tcgggcggcc
    16801 agcccggttc ccagctggtc cagtgccgcg cgtgccagcg gctgttccag aacctttcgg
    16861 aattcaccgg ccatgccctg gccgtccacc tggacacggt gtttgtccgc gagcagcagc
    16921 aggcggagcc gccggccggg gcgttcaact gcgtggcgcg ctgccgcttg agcggcgaac
    16981 tgctcgggcc gcccaactac catgcctaca acgcccggct ccaggagctg tggcgcaccc
    17041 gcttcgcaca catgacgctg gacgaatacc gctcgcacat cgagaccgtc cgcgagccgg
    17101 aacagatcga aaaatggaag cagtcggtct ccgtccgcac ggcataccgg ttcaaggatc
    17161 agcccgagct gccgaccctg tcggaggcgg aggcgcagaa gatctttgcc gagcagcacc
    17221 tcccgaagct ggccttgacc gggcggcggt tcatcatccg ggccgagttg gcccagcagt
    17281 tgacggacca gggcctgcgc cgggccgtcc gggagtcgtg gttccgcgag agccgccatc
    17341 cgtattccgt gatgatggcg ctgcgcccgg ccttccatca catgcatctc tacctcttca
    17401 ggacggccgg cggacagacc ttcgcgacat cgatccagcc gcacccgctc gagccgcggc
    17461 gcgccgtgaa atccatcgcc gaggtgctgc actacctgcg gcggcacccg ggctgcgcgc
    17521 gccacgagtt ggtcgaaaag ctgcggccgg ggatggccgc ggattcgccc caggcggcca
    17581 tggtgttgag cccccttcgc tggctcatcg aaaagggcca cgtgatcgag ttcttcgacg
    17641 ggaccctctc cgtgcccggc gggcctcctt ccgcgcacga accgcacgat cacgctcccg
    17701 gcggcgaggc gcccgccgcg cacccatgaa ccgatcccga atccggcagg ccgtggagcg
    17761 gatgcgcgcc tacacgccgg gcgagcagcc gcaggagccc ggcttcatca agctcaacac
    17821 gaacgagaat ccgtacccgc cgacgccggc gatccgcgag gcgctcgagc gcccgccgga
    17881 ccggttgcgc ctgtacccgg acccgttgtg ccaggaactg cggcgcgagc tggcgcggtt
    17941 gcacggctgc cgggcggagc aggtcttcgt cggcaacggc tcggacgagg tgctcgccct
    18001 gtgcgtccgc gcgttcgtgg agcgggacgg gacgatcggc ttttttgacc cgtcgtattc
    18061 cctctatccc gtgctcgcgg ccgccgagga gatcgccgtg cggcccgtgc ccctgaagcc
    18121 ggatttcacc tgggacatgc ccgcggggta cgcggcgtcg ctgttcttcc tgaccacgcc
    18181 gaacgcgccc acggcgctgc agtatccgcg ggaaacggtc cggtccttct gcgccggatt
    18241 cgacggcgtg gtcgtgctcg acgaggccta cgtggatttc gcgcgggagc atttcatgga
    18301 catggcgctg gaattcggcc acgtcctcgt cgcccgctcg ctgtcgaaat cctactccct
    18361 ggccgggttg cgcgtcgggt atgtcgtggg cgcggagccg ctgatcgccg cgatgtacaa
    18421 gatcaaggat tcctataacg tcgaccgcct ggcgcaggag atcgccctcg ccgcgatccg
    18481 ggaccaggcg cacatgcggg ccaacgcggc gcgaatccgc gcgacgcggg agcgggcggc
    18541 cgcggccctg cggacgcgcg gcttcgaggt cggaccgtcg gaggcgaatt tcctgtgggc
    18601 gcggccgtcc cggccggcgg ccagggacct gtacgaggcc ctgcgggcgc gaaagatact
    18661 ggtgcgctac ttcccggggg agcggaccgg cgcgcacctg cgcgtcacga tcggcacgga
    18721 cggcgagatg gacgcgctgc tgcgcgcgct cgacgagatc ggcgccggct gaccgggggt
    18781 caagcctctt ccacgacttc gtccgccagt tcctgcgcgg cctcgatggc cgtggccttg
    18841 cgatcctccg cgtcgaggtg gctcgggtgc tgcccgtcgg cccacgcgat ggagaaattc
    18901 tggatgccca cggagttcag gatggccgtg agctggggcg tgagctggtc ctgcggatcg
    18961 ccctcgcgca cgatgtccga ggaggaagcg agcaggatgg cccgggtgat gtggtgcagg
    19021 gggatgatgc tgccggcctc gtacttgaag accacgccgg gcaccagcac ctggtccagc
    19081 cacgctttca tgatcgccgg catgccgcag ttccacaggg gcatcgtcag cacgagcac
//
LOCUS       DAOOTK010000052        19067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_1989_length_19067_cov_4.742794, whole genome shotgun sequence.
ACCESSION   DAOOTK010000052 DAOOTK010000000
VERSION     DAOOTK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19067
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_19067_cov_4.742794"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1596)
                     /locus_tag="PK634_07585"
     CDS             complement(145..1596)
                     /locus_tag="PK634_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOW97635.1"
                     /translation="MAALSAQDIEMIARQIAQQLGGAAAPAAAAPRKEEPAATAQPEL
                     GVYGGIDDAVKAAQQAFPKYAALSLQARGKIIAAIRKTMLENATALAKSAHEETGLGR
                     FEDKIIKNQLVAEKTPGVEILTPEAVTGDHGLMLTEPAPFGVIGSITPCTNPTSTIIN
                     NAIAMIAAGNTVVFNPHPSAKKCSAETVALLNKVITANGGPSNVVVCVAQPTIESAGA
                     MMKHPGIRLLVVTGGGGVVKAAMASGKRAICAGPGNPPVVVDETADIEKAARDIVRGA
                     SFDNNIICVEEKEVIVVASVADRLIKAMQQHDAVLIDKARLPQMEQTIFTKMAGPRGH
                     AEINRAFIGKNAEVILSKMGMSVPSTVRLGIVEVEETHPLLWTEQMMPILPITRVPTA
                     DYAIDLAIEMEGHNRHTFIMHSKNLDNLSRMAKECNASIFVKNGPSQAGLGFGGEGCT
                     SFTIASPTGEGLTTPRSFSRWRRCVLVDHFRIV"
     gene            complement(1621..1908)
                     /locus_tag="PK634_07590"
     CDS             complement(1621..1908)
                     /locus_tag="PK634_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOW97636.1"
                     /translation="MNLGRVIGTVVATRKEQSMDGIKFMLVRHVDPEGKETGSFVVAA
                     DAVGAGPDELVLVATGSSARQTVATDKRPCDAVIMAIVDSWDVDGTEKYKK"
     gene            complement(2008..2307)
                     /locus_tag="PK634_07595"
     CDS             complement(2008..2307)
                     /locus_tag="PK634_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOW97637.1"
                     /translation="MADALGMIETRSFPAVVEAADAMVKAAKVELVSYERTGGGYVSV
                     IVRGDVAAVKASCDAGQIAASRVGEVVAVHVIARPHVNVDAVLPLGRAAEAKAKK"
     gene            complement(2361..2993)
                     /locus_tag="PK634_07600"
     CDS             complement(2361..2993)
                     /locus_tag="PK634_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97638.1"
                     /translation="MAELRTYVFLDSLQPQFASFQATVSQGFLPRPGQAALFVEISPG
                     MEINRVTDVALKATNVTPGMQIVERLYGMLEIHSDEQADVRAAGRAILESLGLKEEDR
                     IKPEVTTSQVIRRISDYHTQLINRMRHGNMIEPGQTLYVMECAPAGYAALAANEAEKA
                     ARINVLEVRAFGSFGRVYLGGEERDIDVGYRAAEQAIADITGRPAKGGGG"
     gene            complement(3013..3663)
                     /gene="deoC"
                     /locus_tag="PK634_07605"
     CDS             complement(3013..3663)
                     /gene="deoC"
                     /locus_tag="PK634_07605"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006321050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOW97639.1"
                     /translation="MIDHTILKPDASTADVQKLCNEALKHGFFSVCVNSGYVKQAAAL
                     LRGSPVKVCSVVGFPLGAQPPEIKALEARRAIREGAREIDMVINIGALKSGDDALVLK
                     DIRAVVETCKESRVLCKVILETVLLTNDEKVRACEMSMKARADFVKTSTGFSTGGATA
                     DDIALMAKTVAPKKLGVKASGGVRTYADAMKMIRAGATRIGSSSSIKMLEEAAAAQ"
     gene            complement(3841..4275)
                     /locus_tag="PK634_07610"
                     /pseudo
     CDS             complement(3841..4275)
                     /locus_tag="PK634_07610"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
     gene            complement(4545..6326)
                     /locus_tag="PK634_07615"
     CDS             complement(4545..6326)
                     /locus_tag="PK634_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOW97640.1"
                     /translation="MAMLITKFHSLIRNRLLWAGFLVIIVFTFVIWGTTTSGGKDQEN
                     ANSAGKLDGEYVPMEEFQRSNFSTYMSIVLNVGRPLNVTAELDREIKQAAWRRLAALR
                     QAEALGLIANNDEVIATIRAHQGFQYEGQFSPQHYQAFVQNVLNRLGFSEMQFEEHVR
                     QEIALQKLQYMLQQAVLITPYEINRAIATLSDSFEVDYVAIGEKNVEDEVKVGEDEAR
                     SLFLKDPAAFTIPPKVTLRYVALPLSGFMTNVTATEEEALVYYDDFMDSFVRTNRVRV
                     AEAGAPEGTNTEAEAAWSNEVVQLTFDEVKTNILERMTRERARDKAVEAATELVIQLA
                     PDREGQAPAFEDVMAKAGMEIRTAGPFAENEPVEGVDAGLDFNRAAFALRKTSDEYFS
                     DAIAGSNTVYVLALTERTESRVPEFEEVAKDALEAARRQAISEAITKKAQAVREVALT
                     AKEAKGLTFSDAAQSFKLEPVHVGPFSLTDGLEETNEYNEALLKTVLQLNQGEVSEPV
                     AAEDAVLVIHVAKRGPGDAAGLEPMRDQIVDSIRRQRGRTLFGEWESYLLRKGQLEDR
                     MQVPDEEEPLEENAEPAEENVPPDADA"
     gene            6477..7145
                     /locus_tag="PK634_07620"
     CDS             6477..7145
                     /locus_tag="PK634_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97641.1"
                     /translation="MNKLAMGLGLLALAALLAAGLALRKAAECREEIRVLRAAAPVFV
                     PAPAAPVEQPPPVPDVAPVPVAVSAAGGEVQRVEVAPAAPAAPAPPPLPEHAARDLEN
                     FDRAMDREFDRLDGRAAAPANAAEQDVVTRLKEQLLKLDEIRARAESAAPEENESLRA
                     EMQQVMGSIISLGAAHRRNRIEDLARQAGYDSEQEIAAFLDEVDRIYRETELDWASLF
                     NRGP"
     gene            complement(7122..7850)
                     /locus_tag="PK634_07625"
     CDS             complement(7122..7850)
                     /locus_tag="PK634_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97642.1"
                     /translation="MHIARYPLVLLLALAGDVFPEDTPMPTGPSAEESAPALASNVVA
                     RVGDFDVPYDWFLHEFRSSFFRHTGEPDVREAVFQPFLERMTLYALARHEGLPENPAL
                     RARIRERVDGMRAHMDYQLAMVEVGMVVQAYLESRGLTGDSIQVGDEDLQAFFDRHVK
                     GKPGAPKTLGEVPAEIREQMRQNIAMTKYAETVSAALAGWKTNVPVTVNSSLIQSVPL
                     PEMDGAQPGLPLPAADASGPAVEE"
     gene            7948..9048
                     /gene="aroB"
                     /locus_tag="PK634_07630"
     CDS             7948..9048
                     /gene="aroB"
                     /locus_tag="PK634_07630"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOW97643.1"
                     /translation="MACKIKVDLGARSYEIRVGEGVLAQVGALCRELELGPSALVVSD
                     EHVNPLYGPAVESSLRAAGFQVGRVAVPAGESSKAEARLFRLYDEALDRGLDRRAFVV
                     ALGGGVVGDLAGYFAASYLRGIRYVQVPTSLLAMVDSSVGGKTGINLPRGKNLVGAFH
                     QPVLVAADITTLRSLPPREYAAGLAEVVKYGVIRDAAFFARLEANLAGLRSGEPALLE
                     DVIAHCCRIKAEVVRLDERERSLRAILNFGHTLGHALEQVTGYGTYLHGEAVSLGMAF
                     AARLSTRLAGLPADEAERIENLLRALGLPVDRPDLDWSAARRAMTLDKKTQDRVLKFV
                     LADRIGAARPGVEAPDAVLEEVWTEGRQPKKR"
     gene            9052..9657
                     /locus_tag="PK634_07635"
     CDS             9052..9657
                     /locus_tag="PK634_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97644.1"
                     /translation="MSAVSEWIVREYFEAQGFLVRQPSKYQVSARPKQAEEEIDLLVV
                     NPKASGHVIAEDVLWGVPEIRQVARAVVSVRGWHTERISPKLVARMPELVRFADAEAL
                     KSALRDLGPGPVARILCLPDLPAARPMVSKTLEVLREKGIDGVLLFRPMLLELAAHVD
                     AQKNYEKSDLLQLLRILKKHDLLKDAQLDLFKKGRHASGER"
     gene            9675..10331
                     /locus_tag="PK634_07640"
     CDS             9675..10331
                     /locus_tag="PK634_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW97645.1"
                     /translation="MRTPWRRLLSRIELEGIPWPGACFYNALSRSSPFQKHYALVADE
                     VAVLRPASVLDIGTGPGWLLLRLRERLPGASLHGADISRAMVDKAEKNLPGSGIVLKC
                     AGSEALPYPDASFDVVVSTGSLHHWKNPVAGLNEIRRVLTPGGRALVYDLVRKMPEDV
                     ARRVRAEYGRFRATLMWLHSFEEPFYSAAEMETLAAQSDFAGGETGFTGALCRLRLDR
                     "
     gene            complement(10467..11744)
                     /locus_tag="PK634_07645"
     CDS             complement(10467..11744)
                     /locus_tag="PK634_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97646.1"
                     /translation="MKRVLTIVTGISVVVFFLLHPLCARASEANYQQYLLGDRGAGMG
                     GAACAIAEGVEACYYNPAGLARTPNNTLSLSANLYGFQRLTLDDALFPGEDFEADAFQ
                     TVPSSAGGTYRLGTNLVAGFAVFAPKKMSYFETLAFPESQHYYTLSQDHQVLWLGPSA
                     GWQVSPKLSVGASVYGVYSTFNSQQDLYWGDFGQSYAAGYEYSSLSLLGVAGLQYRWN
                     PLWSLGFRAQTPSALLFSDGKALEHIVGVMDRQLRRDVSYADDVDVDNMTPACFTVGV
                     ARELPKAWTVGADLSCHLAEDFTRLKGTDQNGQPFEVPVERELVVDAAVGAEYVLWKK
                     YPLRAGFFTSRSAAPDPDVDDPYALTQTDLYGVSFSAGTRTRNSAVNIGINYLFGDGT
                     DIGWAQAGGRLEQVLVDVREEYLYFTFNTSYYF"
     gene            complement(11888..15130)
                     /locus_tag="PK634_07650"
     CDS             complement(11888..15130)
                     /locus_tag="PK634_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOW97647.1"
                     /translation="MGGMRHGWLALAVGMALGAPAATLDYASHLGGSDVEYAGGIAVG
                     PAGNVIVAGETGSSGFPSTMEPWSGDRDLFLAKFGTTGSNLIYSTLYGGSGFEGLGGM
                     AFAPNAKIYVAGSVAGAGGYDILVVCFSNSGHVCYNTTVSGPEEDIARHIAVDPDGYA
                     YIVGETGGGLPVTGVAAYGQTYGGGNYDGYLLRLDPTGGVNLCAYLGTTNNDAGYAVA
                     LDASTNVYVALNVGYASRESAYLVKLTSRGSNQQFMAPLYRIYTSNDFARGRALAVDG
                     RTNCYVAGETYTTNFPIAGPAIQPKPGTGSDGFLAIVDGASAGGTVTYSSYFGGAEDQ
                     AATSIVLDGQTNIVVAGENYFFSPFSDIFVARLNSAGSTLLNTVTISEPDHQFSPCIA
                     LDLSSNVWILAETMDDILPTTNAYDRTFAGGFRDAILMQLNWNRGATVTVSSVAATEG
                     TGGTSNFTFKVTMAPTFGAGLAYNYAASNQSAVAGSDFSENRGTNLVFTPGIATQAVT
                     VLVTGDASDESNETFTVNLLQVRNATVAAAQGVGAINDDDPAPSASIADVTVTERDLT
                     QNTNANFVVSLSAASGLIVTMNCATANGTAVAPGDYTNRPSTKLSFNPGESARTVTVV
                     VVGDVMDEDNETFVVNVSGPGNATLADAQGQGTITDNDPAPSFTIGNASTNELDAGQT
                     DSAVVRAALSPPSGKTVTVQWGTVNGTATAGADYASGNGTLTFTAGQTNQAITRPVYG
                     DDLDEFNETFQVTLRTPTNAALLDGTGVVTIVDNDSPPHFILPDSSTNELDAGASNNM
                     AFTVQLSAPSGKPVSVNWGTSNGSAWAGTDYAAGAGLLSLTPGQTNGTVTRPVLGDNL
                     DEFDETFLLTFRTPTNASIADSNAQGAILDNDPPAAASFVDEWLYPLEGDTGSTTVNH
                     RVILYEPSGKTVTVQYATFNASALAGSDYTAAGGALVFAPGQTSFPVACTVLGDTVDE
                     TNEYFTVRVTNIVNGGQDILEERVIIVDDDVDLALLNTLFAAADGTVRLSWPAFTGKT
                     YRVQYHTNLLSGTDWAPLGDDIVAVQTNISTNYPAGTISRRFYRVIQLIP"
     gene            15335..18607
                     /locus_tag="PK634_07655"
     CDS             15335..18607
                     /locus_tag="PK634_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOW97648.1"
                     /translation="MSARSGIPFIIVACLAGRGLVARADTGWVACAIAPPGAVADGAA
                     WRLAGWADTNWQAGASTQAVDAGSYSMEFLDIAGWQAPASRPVTVTAGATTNVDALYM
                     AGRLSMRGTNGAVLASDEAPSTAKGTEYPTLTALRRSATNTFGICNTGDMALAISGVT
                     TSGNGAASFSVVNWPASLAAGETGAFSVIFRPQVTGLLTGRMEIAHNGPGSPFLLRLK
                     GYAEPDMMTIHLFTQRVDGIERNVFSRDSFTVLEVLQTLRDILAGTNLPSVINSFGGH
                     PSYNPHFTEGDTGAFSNDLEYLLGPFMQSGEVFTHAIAVGEKSYVDEFGNYFTTTEPQ
                     QYEDLPSSATNTLIFYAGRMTAAAGVAEWLTNVEIVVRETCYRLRVEDDTFAPPAPDG
                     LAASQGTYSNFVRVAWSNAPFADHYVLWRAPSATGAQTRIGGALTTNVHDDASAATGQ
                     VYCYWVMATNAAGASPASGPVQGWRTMDTNPAVWCAGIRRDGGSGLILQWNASYSNSG
                     YTVEWTESLAAQDWTAWAPTDQWPIVAIVWTAAPPQAAAFFRIRAAPPSNAVIQTLFM
                     DFDGLPVTAGLGKPGSAAPWLYTTATENPLAGTPMTDYDRAGWRNTNAYGPQTNTLSM
                     FYLYYTTDNSVHMGFGTHGYLEIDDGNALSGRSLRYTVTGGVNANGTNGVLVTAKAHF
                     TNSLNLGVDPIAGGVRIGHPYLYFANTSPKETPVPFPEAARANRFSAYFHAPASLTNL
                     DDEARPLATVNIGPYNGIGGHWYHEFCTEGGGWTHALCDGHPQHNNAWAATNYPYPSK
                     SLRDMGEAYFTNWYRWYVTFKPYTGIADAPYDAWFDQVEFLHDPEPQNNETICSPSIT
                     YFPASTNFQIGFMDKYKDNGKSYSTYELRYAFQPIGNAGWSNAVAARILTNAEFGIQA
                     RADGKFQKFNPYYQAVWAPFELAATGDTARLTAGTTVYFAIKDISQVGGDGMLPVTNS
                     GIGPNAIGGRDYSAYSTNFDYAGDQPVLLLIKRTDFRIPAAATNGVLTLTFSPGSMRA
                     AGARWRINGGAWNAKASQWVPEGVYTVEWADVSGWITPPPVTQLVQRGQGPVLTGVYQ
                     SP"
     gene            18696..19064
                     /locus_tag="PK634_07660"
     CDS             18696..19064
                     /locus_tag="PK634_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97649.1"
                     /translation="MNFRCGLGVLAVALALGVTGCEDEDYDHVPPDGQGSIILDNYSP
                     DDIHVYIDGVETNEVKDYEDEAYDMTGGVHRVILDGKDNDRYGAWDVDVVVGQLTVVE
                     IRTSDWNWDDYEVSIHLEEP"
BASE COUNT         2923 a         6338 c         6574 g         3232 t
ORIGIN      
        1 cgatcagggt caggaagcgg cccgggctga tcatcccgct tttgatcacc gagatcggcg
       61 ccttcttgag catggcgtcg gtcgcgtgga tcccgggcgc gatgtcccgg aactcgacga
      121 tggcgatggc ggggtatttc ttcatcagac aatccggaag tggtccacga gcacgcaccg
      181 gcgccagcgc gagaagctgc gcggcgtcgt caggccctcg cccgtcgggc tggcgatggt
      241 gaacgaggtg cagccctcgc cgccgaagcc gaggcccgct tgcgacgggc cgtttttcac
      301 gaagatgctg gcgttgcact ccttggccat ccggctcagg ttgtccaagt tcttggagtg
      361 catgatgaag gtgtggcggt tatgcccctc catctcgatg gccaggtcga tcgcgtagtc
      421 cgcggtcggg acgcgcgtga tcggcaggat gggcatcatc tgctcggtcc agagcagggg
      481 atgggtttcc tcgacctcga cgataccgag ccggacggtg ctgggcacgc tcatccccat
      541 cttggaaagg atgacctcgg cgttctttcc gatgaacgcg cggttgatct ccgcgtggcc
      601 gcgcgggccg gccatcttgg tgaagatggt ctgttccatt tgcggcagac gggccttgtc
      661 gatcagcacg gcgtcgtgct gctgcatggc cttgatcagc cggtcggcga cggaggcgac
      721 gacgatgact tccttctcct cgacgcagat gatgttgttg tcgaacgagg cgccgcggac
      781 gatgtcgcgc gcggccttct cgatgtccgc ggtctcgtcc acgaccaccg gcgggttgcc
      841 cggcccggcg cagatcgccc gcttgccgct ggccatggcg gccttgacca cgccgccgcc
      901 gcccgtgacg acgagcaggc ggatgcccgg gtgcttcatc atcgccccgg cgctctcgat
      961 cgtcggttgg gccacgcaga ccacgacgtt cgacggcccg ccgttcgccg tgatgacctt
     1021 gttcagcagc gccaccgtct ccgcagagca cttcttcgcg gacgggtggg ggttgaacac
     1081 gaccgtgttg ccggccgcga tcatggcgat ggcgttgttg atgatcgtcg aggtcgggtt
     1141 cgtgcagggc gtgatcgagc cgatgacgcc gaagggcgcg ggctcggtga gcatcaggcc
     1201 gtggtcgccg gtgacggcct cgggggtcag gatctcgacg ccgggggtct tctcggcgac
     1261 gagctggttc ttgatgatct tgtcctcgaa ccggccgagc cctgtctcct cgtgggccga
     1321 cttggcgagg gcggtcgcgt tctccagcat ggtcttgcgg atcgccgcga tgatcttgcc
     1381 tcgggcctgg agcgagaggg cggcgtactt cgggaacgcc tgctgggcgg ccttgaccgc
     1441 gtcgtcgatg ccgccgtaca cgcccagttc gggctgcgcg gtcgcggccg gctcctcctt
     1501 gcggggcgcc gcggcggcgg gtgcggcggc gccgcccaac tgctgggcga tctgccgggc
     1561 aatcatctca atatcttgag cggagagcgc tgccatgact caatctcctt gcgcggcccg
     1621 tcacttcttg tacttctcgg tcccgtccac gtcccagctg tccacgatgg ccatgatcac
     1681 ggcgtcgcag ggccgcttgt ccgtggcgac ggtctgccgt gcggagctgc ccgtggcgac
     1741 caggaccagc tcgtccggcc ccgcgccgac cgcgtcggcg gcgaccacga agctcccggt
     1801 ctccttgccc tcggggtcca cgtgccggac gagcatgaac ttgatgccgt ccatgctctg
     1861 ctccttgcgc gtggccacga ccgtgccgat gactctgccc aggttcatgc gtatctcccg
     1921 tgtgaatggc gagtagtgaa tggtgaatgg tcggatagcc atcaaccatt cactatcaac
     1981 catctcctcc gccggccgcc ggcgggctta cttcttggcc ttggcttcgg ccgcgcgacc
     2041 cagcgggagc accgcgtcca cgttgacgtg cgggcgcgcg atgacgtgga cggcgaccac
     2101 ctcgccgacc cggctcgctg cgatctggcc ggcgtcgcag gacgccttga ccgccgcgac
     2161 atcgccgcgg acgatcacgc tgacgtagcc gccgccggtc cgctcgtagc tgaccagctc
     2221 gaccttcgcc gccttgacca tggcgtcggc cgcctcgacc accgccggga acgaacgggt
     2281 ttcaatcatg cccaatgcat ctgccatgtt ctcttctcct tttttcgtac ggtttgctta
     2341 acattgttct cgagcgcaga tcagcccccg ccgcccttcg ccggccggcc ggtgatgtcc
     2401 gcgatcgcct gctcggcggc gcggtagccg acgtcgatgt cccgctcctc gccgccgagg
     2461 tagacgcggc cgaagctgcc gaatgcgcgg acctcgagga cgttgatgcg ggcggccttc
     2521 tcggcctcgt tcgcggcgag ggccgcgtag ccggccggcg cgcactccat gacgtagagc
     2581 gtctggcccg gctcgatcat gttgccgtgc cgcatgcggt tgatgagctg ggtgtggtag
     2641 tccgagatac gccggatcac ctggctcgtg gtgacctcgg gcttgatccg gtcctcctcc
     2701 ttcaggccca gcgactcgag gatcgcccgg cccgcggcgc ggacgtccgc ctgctcgtcg
     2761 gagtggatct cgagcatgcc gtacaaccgc tcgacgatct gcatgccggg cgtgacgttg
     2821 gtcgccttga gcgccacgtc ggtgacgcgg ttgatctcca tgcccggcga gatctcgacg
     2881 aacagggcgg cctgccccgg gcgggggagg aacccctgcg acaccgtcgc ctggaacgac
     2941 gcgaactgcg gctgcaggct gtccaggaat acgtaggttc ttagttcggc catggctgtc
     3001 ttcctttccc gcctactggg cggcggctgc ttcctccaac atcttgatgc tgctgctcga
     3061 gccgatgcgc gtcgcgccgg cgcggatcat cttcatcgcg tcggcgtacg tccggacgcc
     3121 cccggaggcc ttgacgccga gcttcttcgg ggcgacggtc ttcgccatca gggcgatgtc
     3181 gtcggccgtc gcgccgccgg tgctgaagcc ggtggacgtc ttcacgaaat cggcgcgcgc
     3241 cttcatgctc atctcgcagg cgcggacctt ctcgtcgttc gtcaggagaa cagtttcgag
     3301 gatcaccttg cacagcaccc gcgattcctt gcaggtctcg accacggcgc ggatgtcctt
     3361 gagcaccagc gcgtcgtcgc cgctcttcag ggcgccgatg ttgatgacca tgtcgatttc
     3421 cctggcgcct tcgcggatcg cgcggcgggc ctcgagggcc ttgatctccg ggggctgggc
     3481 ccccagcggg aacccgacga ccgagcagac cttgaccggc gagccgcgca ggagggcggc
     3541 ggcctgcttg acgtagcccg agttcacgca gacggagaag aacccgtgct tcagggcctc
     3601 gttgcagagt ttctggacgt ccgccgtcga ggcgtccggc ttgaggatgg tgtggtcgat
     3661 catcctggcg agatccttcg gcgccagttt cgcgggctgg gccgacggga ccgcggccgg
     3721 ggcgccggcg aggatatcct tcacgcgggc ggtgatcttc gcaatatctt caggcgagag
     3781 gtccaaggcg ttctcctttt cgttccgggc cgggccgccg gcccagtgtt tctccaggtc
     3841 cgcgatcatc tggacccgct tcaggtgccg ttcggccgtg cactcggtgt ggatgaaaat
     3901 gtccgcgatc gccttgacct ggtccagact attctggccc gcgccgaggg tcaagacgtt
     3961 cgcgccgttg tgctcgcggc agttgcgggc gagggcctcg ttgtagcacg ccgccgcgcg
     4021 cacgccggga attttgttgg ccgtcatggc ggagccgatg cccgcgccgt cgatcatgat
     4081 gccgatgtcg cactggccgg acgagaccag ccgggctacc gcggcggcga tgcgggggta
     4141 atccaccgcg tccttgctgt gggtgccgca gtcctggatc ccgtgtccct ggcccttgag
     4201 ccaggcggcc agggcttcct tcatctcgta gccgccgtga tcggcgccca cggcgatgcg
     4261 caatggcttg ctcatagacg aagcgtgact tatgccactt cgtccccgtg aagacaagag
     4321 cggttttcgc cgaaatcgcc gctgtatttc ataacaatcg cggcggggca ttaccggtgc
     4381 atcccgcacc atcccgccaa gggcagcggg ccgggcgacc cggacccgcg gagcagcatg
     4441 gccaccgacg gggggaccag atacctccgg cgggacctca cgggcctggt cctcgccgat
     4501 ctgggtcgag cggccgtagg ggccggccgc cgggcgaagg aggatcaggc gtcggcgtcc
     4561 ggcggaacgt tctcttcggc cggctcggcg ttctcttcca acggctcctc ctcgtccggc
     4621 acctgcatcc ggtcctcgag ctggcctttc cggaggagat aagattccca ctcgccgaac
     4681 agcgtccgcc cgcgctggcg ccggatggag tcgacgatct ggtcgcgcat gggttcgagg
     4741 ccggcggcgt cgcccgggcc ccgcttcgcc acgtggatca cgaggaccgc gtcctcggcc
     4801 gcgaccggtt cgctgacctc gccctggttg agctggagca ccgttttcag cagcgcctcg
     4861 ttgtactcgt tcgtctcctc gaggccgtcg gtcaagctga acgggccgac atgaacgggc
     4921 tccagcttga aggattgcgc ggcgtcgctg aacgtcaggc ccttggcctc cttcgcggtc
     4981 agcgccacct cccggacggc ttgcgccttc ttcgtgatgg cctcgctgat cgcctgccgg
     5041 cgcgccgcct cgagcgcgtc cttcgccact tcctcgaatt cgggaacgcg ggactccgtg
     5101 cgctccgtca gcgccaagac gtacaccgtg ttgctgccgg cgatggcgtc gctgaagtac
     5161 tcgtcggagg ttttgcgcag ggcgaaggcg gcccgattga agtcgaggcc ggcgtccact
     5221 ccttccacgg gctcgttttc cgcgaagggc ccggcggtcc ggatttccat gccggccttg
     5281 gccatcacgt cctcgaacgc cggggcctgg ccttcccggt cgggcgcgag ctggatcacg
     5341 agttccgtcg cggcctccac ggccttgtcc cgggcgcgct cgcgggtcat ccgctcgagg
     5401 atgttggtct tgacctcgtc gaaggtcagc tgcacgacct cgttggacca ggcggcctcg
     5461 gcctccgtgt tcgtgccctc gggcgcgccg gcctccgcga cgcggacgcg gttggtgcgc
     5521 acgaacgaat ccatgaaatc atcgtagtaa accagcgcct cctcctcggt cgccgtcacg
     5581 ttggtcatga agccggacag cggcagcgcg acgtagcgca gggtgacctt cggcgggatc
     5641 gtgaacgcgg cgggatcctt caggaagaga gagcgggcct cgtcctcgcc gaccttgacc
     5701 tcgtcctcga cgttcttctc gccgatcgcc acgtagtcga cctcgaagga atcgctcaag
     5761 gtggcgatgg cccggttgat ctcgtagggg gtgatcagga cggcctgctg gagcatgtac
     5821 tggagtttct ggagggcgat ctcctgccgg acgtgttcct cgaactgcat ctcggagaag
     5881 cccaggcggt tcaggacgtt ctggacgaag gcctggtagt gctgcgggct gaactggccc
     5941 tcgtactgga agccctggtg cgcgcggatg gtggcgatga cctcgtcgtt gttggcgatc
     6001 aggccgaggg cctcggcctg acggagcgcc gccagccggc gccacgcggc ctgcttgatc
     6061 tccctgtcca gctccgcggt gacgttcagg ggccggccga cgttcaggac gatggacatg
     6121 tacgtgctga agttcgagcg ctggaattcc tccatgggca cgtactcgcc gtccagtttc
     6181 ccggccgagt tggcgttttc ctggtccttc ccgcccgagg tggtggtccc ccagatgaca
     6241 aaggtgaaga cgatgatcac gaggaacccc gcccacagca ggcggttccg gatcagactg
     6301 tgaaacttgg taatcagcat ggccatgatc gcacgcgccc tttctgtcta ccctgaacct
     6361 ggtacgaaac gccgaaacat aaacacccgc ccccgcccgg tcaacagcga atcggccggg
     6421 cccgcggcgc ccggattgag ttggcaggcc ggggcggatg gtgtatggga ggccgcatga
     6481 acaagcttgc catgggcctg ggcctgctgg ccctggccgc gctgctggcg gccggtctcg
     6541 cgctgcggaa ggccgcagaa tgcagggagg aaatccgtgt cctgcgggct gcggccccgg
     6601 tgttcgttcc cgcgccggcc gctccggtcg aacagccgcc gcccgtgccg gacgtcgcgc
     6661 cggtacccgt ggcggtgtcg gccgccggcg gcgaagtgca gcgggtggaa gtggcgcccg
     6721 cggctcccgc cgcgccggcg cccccgccgc tgccggaaca cgcggcgcgc gacctggaga
     6781 atttcgatcg ggcgatggac cgggaattcg accggctgga cggccgggcg gcggcgccgg
     6841 cgaacgccgc cgagcaggac gtggtgacgc ggctcaagga gcagttgctc aagctggacg
     6901 agatccgggc ccgcgccgaa agcgcggcgc cggaggaaaa tgaatcgctg cgcgcggaaa
     6961 tgcagcaggt catgggctcc atcatttccc tcggcgccgc gcaccggcgg aaccggatcg
     7021 aggacctggc gcggcaggcc ggctacgaca gcgaacagga gatcgcggcg ttcctcgacg
     7081 aggtggaccg gatctaccgc gagacggaat tggactgggc gtcactcttc aaccgcgggc
     7141 cctgatgcgt cggccgctgg caagggcagg cccggctgcg cgccgtccat ctccggaagc
     7201 ggcacggatt ggatcaggct cgagttgacc gtcaccggca cgttcgtttt ccagccggcc
     7261 agggcggcgc tgacggtctc ggcgtatttc gtcatggcga tgttctggcg catctgttcc
     7321 cggatctccg ccgggacttc gcccagcgtt ttcggagcgc cgggcttccc cttgacgtgc
     7381 cggtcgaaga aggcttggag gtcctcgtcg ccgacctgga tggaatcccc cgtcaggccg
     7441 cgggattcga ggtacgcctg cacgaccatg cccacctcga ccatggccaa ctggtaatcc
     7501 atgtgcgcgc gcatgccgtc gacgcgctcc cggatccgcg cgcgcagcgc ggggttctcg
     7561 gggaggccct cgtgccgggc gagggcgtag agggtcatgc gctcgaggaa cggctggaag
     7621 accgcctcgc ggacgtccgg ctcgccggtg tggcggaaga aggaggagcg gaactcgtgc
     7681 aggaaccagt cgtatggcac gtcgaagtcg ccgacgcggg cgacgacgtt ggaagccagg
     7741 gcgggggcgg attcctccgc cgaggggccc gtcggcatcg gggtgtcttc agggaaaacg
     7801 tccccggcga gggccaggag caggaccagc gggtagcgcg cgatgtgcat gggggcacgg
     7861 tagcgcggaa cccgcgcggt ggcaacaggg aagaggggct ctatgatgtt gacaccggcc
     7921 agccccggcc ctatttgctc atcattgatg gcctgtaaaa tcaaagtgga tctcggcgcg
     7981 cgctcctacg aaatacgcgt cggggaaggc gtgctggcgc aggtcggcgc gctgtgccgc
     8041 gagctcgagt tgggcccctc cgccctggtc gtttccgacg agcacgtgaa tccgctgtac
     8101 ggcccggcgg tggagtcgtc cctgcgcgcg gccgggttcc aggtcgggcg ggtggccgtc
     8161 cccgcgggcg agtcgtccaa ggccgaggcc cggctgttcc gcctgtacga cgaggcgctg
     8221 gaccgcggcc tggaccgccg ggcctttgtc gtcgcgctcg gcggcggggt ggtgggcgac
     8281 ctcgcgggct actttgcggc cagctacctg cgcggcatcc gctacgtcca ggtcccgacc
     8341 agcctgctcg cgatggtgga cagctccgtc ggcggcaaga cgggcatcaa cctgccgcgc
     8401 ggcaagaacc tcgtgggcgc gttccaccag ccggtcctgg tcgcggccga catcaccacg
     8461 ctgcgctcgc ttccgccgcg cgagtacgct gccgggctgg ccgaggtggt gaaatacggc
     8521 gtgatccgcg atgccgcttt cttcgcacgc ctcgaggcga acctcgccgg gctgcggtcg
     8581 ggcgagcccg cgctgctgga agacgtcatc gcccactgct gccggatcaa ggcggaggtg
     8641 gtgcggctgg acgagcgcga gcgcagcctg cgcgcgatcc tgaatttcgg gcataccctg
     8701 ggccacgccc tcgagcaggt caccggctac gggacttacc tgcacggcga ggcggtcagc
     8761 ctgggcatgg ccttcgcggc gcggctttcg acgcgtctcg ccggcctgcc cgcggatgag
     8821 gcggaacgca tcgagaacct gctgcgggct ctcggcctgc cggtcgaccg gccggacctc
     8881 gactggtccg cggcgcgccg ggccatgacg ctggacaaga agacgcagga ccgggtgctg
     8941 aaattcgtgc tggccgaccg gatcggcgcc gcgcggcccg gcgtcgaagc gccggatgcc
     9001 gtgctggagg aagtctggac cgagggacgt cagccgaaga aaaggtgagc tatgtccgcc
     9061 gtcagcgaat ggatcgtccg tgaatatttc gaggcgcagg gcttcctggt gcgccagccg
     9121 tcgaagtacc aggtctccgc gcggcccaag caggccgagg aggaaatcga cctgctggtc
     9181 gtcaacccga aggcctcggg gcatgtcatc gcggaggatg tcctgtgggg cgtcccggag
     9241 atccggcagg tggctcgcgc ggtcgtcagc gtgcgcggct ggcacacgga acgcatcagc
     9301 ccgaagctgg tcgcgcggat gcccgaactt gtccggttcg ccgacgcgga ggcgctgaaa
     9361 tccgcgctgc gcgacctggg gccggggccc gtcgcgcgca tcctctgcct gcctgacctg
     9421 ccggccgcgc gcccgatggt gtcgaagacc ctggaggtcc tgcgggagaa ggggatcgac
     9481 ggcgtgctgc tgttccgccc gatgctcctg gaactcgcgg cccacgtgga cgcgcagaaa
     9541 aactacgaga aatccgacct gctccagctc ctgcgtatcc tgaagaaaca cgacctgctc
     9601 aaggacgccc agctcgacct gttcaagaag ggccggcacg cctccggaga gcggtagcaa
     9661 ccgccgggag cggcatgcgc acgccctggc gcagactgtt gtcccggatc gagctggagg
     9721 gcatcccctg gcccggcgcc tgtttttaca acgccttgag ccggagttcg cccttccaga
     9781 agcactatgc cttggtggcg gacgaggtcg ccgtgctgcg gccggcgtcg gttctcgaca
     9841 tcgggaccgg gccgggctgg ctgctgctcc gcctgcgcga gcggctgccg ggcgcgtcgc
     9901 tccacggggc ggacatctcg cgggcgatgg tggacaaggc cgagaagaac ctgccgggga
     9961 gcggcattgt actgaagtgc gccggctcgg aggcgctgcc gtatcccgac gcctccttcg
    10021 acgtcgtcgt gtccaccggt tccctccatc actggaagaa tcccgtggcg gggctgaacg
    10081 agatccggcg cgtcctcaca cccggcgggc gcgcgctggt ctacgacctg gtccgcaaga
    10141 tgccggaaga cgtcgcccgc cgcgtgcggg cggaatacgg gcggttccgc gcgacgctga
    10201 tgtggctgca ctcgttcgag gagccgttct acagcgccgc ggaaatggaa acgctcgccg
    10261 cgcaaagcga cttcgcgggc ggggagaccg ggttcaccgg cgcgctctgc cggttgcggc
    10321 tcgaccggta atgcgagcct tcgtgaacgt cgcttgaaaa aacggcgtcg cggagctccg
    10381 ccctccatgg cgtacctggc ccggtgcttg gtggagggtc gagctcccgc gagaccgggc
    10441 gcgtctatac cggcctgatg gatacgtcag aagtagtacg acgtgttgaa ggtgaagtag
    10501 aggtactcct cccgcacgtc cacgaggacc tgctcgaggc ggccgccggc ctgggcccag
    10561 ccgatgtcgg tgccgtcgcc gaagaggtag ttgattccga tgttgaccgc gctgttccgc
    10621 gtgcgcgttc cggcgctgaa gctgacgccg tacaggtcgg tctgggtcag cgcgtaggga
    10681 tcgtccacgt ccgggtcggg ggcggcggac cggctggtga agaacccggc gcgcagcggg
    10741 tatttcttcc agagcacgta ctcggcgccg accgcggcgt ccacgaccag ctcgcgctcc
    10801 acgggcacct cgaagggctg gccgttctgg tcggtgcctt tgagccgcgt gaagtcctcc
    10861 gcgagatggc aggagaggtc cgcgccgacc gtccaggctt tcgggagctc gcgcgcgacg
    10921 ccgactgtga aacaggccgg agtcatgttg tccacgtcga cgtcgtcggc atacgacacg
    10981 tcccgccgca actggcggtc catcacgccg acgatatgct ccagcgcctt gccgtcgctg
    11041 aagagcaggg cggacggggt ctgcgcccgg aagccgaggc tccagagcgg attccaccgg
    11101 tactgcaggc cggccacgcc gagcagggac aggctgctgt actcgtagcc cgccgcgtac
    11161 gattgcccga aatcgcccca gtacaggtcc tgctgggagt tgaacgtgct gtagacgccg
    11221 tacacggacg cgccgacgga cagcttcggg gaaacctgcc agcccgccga cggcccgagc
    11281 cacaggacct ggtggtcctg gctcaacgtg tagtagtgct gcgactcggg gaaagccagg
    11341 gtctcgaagt acgacatctt cttcggcgcg aacaccgcga agcccgcgac gagattcgtg
    11401 cccagccggt acgtgccgcc cgccgaggac ggcacggtct ggaacgcgtc ggcctcgaaa
    11461 tcctcgccgg ggaacagggc gtcatccagt gtcaggcgct ggaagccgta gaggttggcc
    11521 gagagcgaca gcgtgttgtt cggcgtgcgc gccaggccgg ccggattgta atagcaagcc
    11581 tccacgcctt ccgcgatcgc gcaggccgcg ccgcccatgc cggccccccg gtcgccgagc
    11641 aggtactgct ggtagttggc ctccgaggcc cgcgcgcaga ggggatggag cagaaaaaag
    11701 acgaccacgc tgatgccggt cacgatcgtc aaaacgcgct tcatgagggc ctcctttgca
    11761 gtacgagatg ctagccgaag catcgggtct tgtgaagcct gacatagagg gatcgaaggc
    11821 gcccgcagga cacatcacgg cgcggacgga gtgttgtgcg caggatgtcg ctgcccggga
    11881 aggctgatca gggaatcagc tggatcacgc ggtaaaaccg ccgggagatg gttcccgccg
    11941 ggtagttggt gctgatgttg gtctgcaccg caacgatatc gtcacccagc ggagcccagt
    12001 cggtcccgct caagagattg gtgtgatatt gcacgcggta ggtcttcccg gtgaacgcgg
    12061 gccaggagag ccggacggtg ccgtccgcgg cggcgaagag cgtgttcagc agggccaggt
    12121 ccacgtcgtc gtccacgatg atcacccgct cctccaggat gtcctggccc ccgttgacga
    12181 tgttggtgac ccggacggtg aaatactcgt tggtctcgtc caccgtgtcg ccgaggacgg
    12241 tgcacgccac ggggaaactg gtctgtccgg gcgcgaagac cagggcgccg cccgcggcgg
    12301 tgtagtccga gccggccagc gccgaggcgt taaaggtcgc gtactgcacc gtcaccgtct
    12361 tgccgctggg ttcgtacagg atcacccggt ggttcacggt cgtcgagccc gtgtcgccct
    12421 cgagcgggta gagccactcg tccacgaagc tcgcagccgc cggcggatcg ttgtccagga
    12481 tggcgccctg ggcgttgctg tccgcgatgg acgcgttagt cggcgtgcgg aacgtcagca
    12541 ggaaggtctc gtcgaactcg tcgaggttgt cgcccaggac cggccgcgtc acggtcccgt
    12601 tggtctgccc gggcgtgagc gaaaggagac ctgccccggc ggcatagtcc gttccggccc
    12661 atgcggagcc attgctcgtg ccccagttca cggagacggg tttgccgctg ggggccgaca
    12721 gttggaccgt gaaggccatg ttgttgctgg cgccggcgtc gagttcgttc gtgctcgaat
    12781 cgggcagtat gaaatgggga ggggagtcgt tgtccacgat ggtcacgacg ccggtgccgt
    12841 ccagcagcgc cgcgttggtg ggggtccgca gggtgacctg gaacgtctcg ttgaactcgt
    12901 ccaggtcgtc gccgtacacg ggacgggtga tggcttggtt cgtctgcccg gcggtgaacg
    12961 tcagcgtgcc gttgccgctg gcgtagtccg cgcccgccgt cgccgtgccg ttcaccgtgc
    13021 cccactggac ggtcaccgtc ttcccgctgg gcggggacag cgccgcccgg acgacggcgg
    13081 aatccgtctg gccggcatcg agctcgttcg tcgaggcatt gccgatcgtg aaggatggcg
    13141 cggggtcatt gtcggtgatc gtgccctggc cctgggcgtc cgcgagggtg gcgttgccgg
    13201 gtccgctcac gttgacgacg aaggtctcgt tgtcctcgtc catgacgtcg ccgacgacca
    13261 cgacggtcac ggtccgggcc gactcgccgg gattgaacga gagcttggtc gagggccggt
    13321 tggtgtagtc gccgggagcg acggccgttc cattcgccgt ggcgcagttc atggtcacga
    13381 tcagcccgct ggcggcggac aggctcacga cgaaattcgc gttggtgttc tgggtgaggt
    13441 cccgctcggt gaccgtcacg tcggcgatgg aggcggacgg cgcggggtcg tcgtcgttga
    13501 tggcgccgac cccctgggct gccgccacgg tcgcgttccg gacctgcaag agattgacgg
    13561 tgaacgtctc gttggactcg tccgaggcgt cgcccgtcac caggaccgtg accgcctgcg
    13621 tggcgatccc gggcgtgaag accaggttgg tgccccggtt ctcgctgaag tcggagcccg
    13681 cgacggcgga ctggttggag gccgcgtagt tgtaggccaa ccccgcgccg aaggtcggcg
    13741 ccatcgtgac cttgaaggtg aaattgctgg tccctcccgt cccctccgtg gccgcgacac
    13801 tgctcacggt caccgtggcg ccgcggttcc aattcagctg catcaggatc gcgtcgcgga
    13861 aaccgccggc gaaggtcctg tcgtaggcgt tggtcgtcgg gaggatgtca tccatcgttt
    13921 ccgcgaggat ccagacgttg ctggagaggt cgagggcgat gcatggcgag aactggtggt
    13981 ctggttcgct gatcgtgacg gtgttcagca gcgtggatcc cgcgctgttc agccgcgcca
    14041 cgaagatgtc gctgaacggg ctgaagaagt agttctctcc cgcgaccacg atgttggtct
    14101 gcccgtcgag gacaatcgag gtggcggcct ggtcctccgc gccgccgaag taggaggagt
    14161 aggtgacggt gcccccggcg gaggccccgt cgacgatggc gagaaagcca tccgagccgg
    14221 tgcccggctt aggctggatc gcgggcccgg cgatcgggaa attcgtggtg tacgtctctc
    14281 ccgcgacgta gcagttcgtc cgcccgtcca cggcgagggc gcggccgcgg gcgaagtcgt
    14341 tgctcgtata aatccggtag aggggcgcca tgaactgctg gttggagccg cggctggtca
    14401 gcttgaccag gtaggcggac tcgcggctgg catacccgac attcagggcg acgtacacgt
    14461 tcgtggaggc gtcgagcgcc acggcgtacc cggcgtcatt gttcgtggtg ccgagatagg
    14521 cgcagagatt gacgccgccg gtcggatcga ggcggagaag gtacccgtcg taattgccgc
    14581 ccccgtaagt ctgcccgtaa gccgccacgc cggtcacagg caggccgccg ccggtttctc
    14641 cgacgatgta ggcgtagcca tccgggtcca cggcaatgtg gcgggctatg tcctcctccg
    14701 gtcccgagac cgttgtgttg tagcacacgt gtccgctgtt gctgaagcag accaccagga
    14761 tgtcgtagcc gccggcgccc gcgaccgagc cggcgacgta gatcttggcg ttcggcgcga
    14821 aggccatgcc gccgagcccc tcgaagccgc ttccgccgta cagcgtggag tagatcaggt
    14881 tgctgcccgt cgtgccgaac ttggctagaa aaagatcccg gtcgccgctc caaggttcca
    14941 tcgtggaggg gaagcccgac gacccggtct cgccggcgac gatcacgttg cccgcgggcc
    15001 cgaccgcgat gcctcccgcg tactccacgt ccgaaccgcc gagatgcgag gcatagtcca
    15061 gcgtcgccgc cggcgcgccc agcgccattc cgacggccag cgccagccat ccatgccgca
    15121 tgccgcccat cgtcgtgttc ctcctgaaaa gccctttccg ccccaaggat atcggccgcg
    15181 tcaggaactg cacggaaaaa agatcagggc gggccggctc ctcgcttgcc ctgtgcgcaa
    15241 gccccgggta ggctggccgt tgagcggtat gccgtccatc cgggctccgg agagggcggc
    15301 gtgccgggga ggaaaagggg aacccgagta cccgatgagc gcacgaagcg gcattccttt
    15361 tatcatcgtc gcgtgcctgg ccggccgggg cctcgtcgcg cgggccgaca cgggctgggt
    15421 cgcctgcgcc atcgccccgc ccggcgccgt ggccgacggg gccgcgtggc ggctggccgg
    15481 ctgggccgac acgaactggc aggccggcgc ctcgacgcag gccgtggacg ccggcagcta
    15541 ctccatggag ttcctggaca tcgccggctg gcaggcgccc gcctccaggc ccgtaaccgt
    15601 gaccgccggc gccacgacca acgtggacgc gctgtacatg gccggacgcc tctcgatgcg
    15661 gggcaccaac ggcgccgtcc tcgccagcga cgaagccccg tcgacggcga agggcaccga
    15721 gtatcccacc ctgacggccc tgcggcggtc ggcgacgaac acgttcggca tctgtaacac
    15781 cggcgacatg gcgctcgcga tcagcggggt gaccaccagt gggaacgggg ccgcttcgtt
    15841 ctccgtcgtg aactggccgg cgtccctggc cgccggcgag acgggcgcct tcagcgtcat
    15901 cttccggccc caggtcactg gcctgctgac cgggcgaatg gagatcgcgc acaacggccc
    15961 gggctccccg ttcctcctcc gcctcaaggg ctacgcggaa ccggacatga tgaccatcca
    16021 cctcttcacg cagcgcgtgg acggcatcga gcgcaacgtc ttctcgcgcg acagcttcac
    16081 ggtgctcgag gtcctgcaaa ccctccgcga catcctcgcc gggaccaacc tgccctccgt
    16141 gatcaacagc ttcggcggcc acccgagcta caacccgcac ttcacggaag gcgacacggg
    16201 ggccttcagc aacgatctgg agtatctgct ggggccgttc atgcagtcgg gcgaggtgtt
    16261 cacccatgcg atcgcggtgg gcgagaagtc gtacgtggac gagttcggca actacttcac
    16321 caccacggag ccgcaacagt acgaggacct gccctcctcg gcgaccaaca cgctgatctt
    16381 ctatgccggc cgtatgaccg ccgccgcggg cgtcgccgag tggctgacga acgtcgagat
    16441 cgtcgtgcgc gagacgtgct accgccttcg cgtggaggac gacacgttcg cgccccccgc
    16501 gccggacggc ctggcggcct cgcaggggac ctattctaac ttcgtgcggg tcgcctggtc
    16561 gaacgcgccg ttcgcggacc actatgtcct ctggcgcgcg ccgtccgcga ccggcgccca
    16621 gacgcgcatc gggggggcgc tgaccacgaa cgtccacgac gacgcctcgg ccgccacggg
    16681 ccaggtgtac tgctactggg tcatggcgac gaacgcggcg ggcgcgagcc cggccagcgg
    16741 ccccgtccag ggttggcgga cgatggacac gaacccggcg gtctggtgcg cgggcatccg
    16801 gcgggacggc gggagcggcc tgatcctgca gtggaacgcc tcgtacagca actcgggcta
    16861 cacggtcgaa tggacggaat cgctggcggc gcaggactgg acggcgtggg cgccgacgga
    16921 ccagtggccg atcgtggcga tcgtctggac ggccgccccg ccgcaggcgg ccgctttctt
    16981 ccgcatccgg gcggcgccgc cctccaacgc cgtcatccag accctcttca tggatttcga
    17041 cggactgccg gtcaccgccg ggctcggcaa gccgggctcc gcggcgcctt ggctctacac
    17101 gacggccacc gagaacccgc tagccggcac ccccatgacg gattacgacc gcgcgggctg
    17161 gcgcaacacg aacgcgtacg ggccgcagac aaacaccctc tccatgttct atctctacta
    17221 cacgaccgac aacagcgtgc acatgggttt cggcacgcac ggctacctgg agatcgacga
    17281 cgggaacgcc ctttccggcc gcagcctgcg ctacacggtc accggcggcg tcaacgcgaa
    17341 cggcacgaac ggggtcctcg tcaccgccaa ggcccatttc acgaacagcc tgaacctggg
    17401 cgtcgatccg atcgccggcg gcgtcagaat cgggcatccc tacctctact tcgcgaacac
    17461 cagcccgaag gagacgccgg tcccgttccc cgaagccgcg cgcgcgaacc gcttcagcgc
    17521 gtacttccac gcgcccgcct cgctgaccaa tctcgacgac gaggcgcgcc cgctggccac
    17581 ggtcaacatc ggcccgtaca acgggatcgg cggccactgg taccacgagt tctgcacgga
    17641 gggaggcggc tggacccacg cgctgtgcga cgggcatccc cagcacaaca atgcgtgggc
    17701 ggcgaccaac tacccgtacc ccagcaagag cctgcgcgac atgggcgagg cgtacttcac
    17761 caactggtat cgctggtacg tcacgttcaa gccctacacc gggatcgccg acgcgccgta
    17821 cgacgcctgg ttcgaccagg tcgagttcct gcacgatccg gagccgcaga acaacgagac
    17881 catctgctcc ccgtccatca cctacttccc cgcgtcgacc aacttccaga tcggcttcat
    17941 ggacaagtac aaggacaacg ggaagagcta ctctacgtac gagctgcgct acgccttcca
    18001 gccgatcggg aacgcgggct ggtccaacgc cgtcgcggcg cgcatcctca ccaatgccga
    18061 gttcgggatc caggcgcgag ccgacgggaa gttccagaag ttcaacccgt actaccaggc
    18121 ggtctgggcg cccttcgaac tcgccgccac cggcgacacg gcccggttga ccgcgggaac
    18181 gaccgtctac ttcgcgatca aggacatttc gcaggtgggc ggagacggca tgctgccggt
    18241 caccaacagc ggcatcggcc cgaacgcgat cggcgggcgc gactactccg cgtacagcac
    18301 caacttcgat tacgccggcg accagccggt cctgctgctc atcaagcgaa cggacttccg
    18361 gatccccgcc gccgccacca acggcgtcct gaccctgacc ttctcgccgg gttcgatgcg
    18421 cgcggccggc gcccgatggc ggatcaacgg cggcgcttgg aacgcgaagg cctcacagtg
    18481 ggttcccgaa ggcgtgtaca ccgtcgagtg ggcggacgtc agcggctgga tcacccctcc
    18541 gcccgtcacc cagctcgttc agcggggcca gggccccgtc ctgaccggcg tgtaccagtc
    18601 cccgtgaggc cgcgcggagc aggcggttct tgcattcggg ggcggggttt tctaccattc
    18661 gaacaggagc ccgcgatggg gaaaggatga gcaccatgaa ctttcgttgc ggattgggcg
    18721 tgctggccgt ggcgctggcg ctgggggtga ccgggtgcga ggacgaggac tacgaccacg
    18781 ttccgccgga cgggcagggg agcatcatcc tcgacaatta ctcgcccgac gacatccacg
    18841 tctatatcga cggcgtggag accaatgagg tgaaggacta cgaggacgag gcttacgaca
    18901 tgaccggcgg cgtccaccgc gtgatcctcg acggcaagga caacgaccgt tacggcgcct
    18961 gggacgtgga cgtcgtcgtc gggcaactca ccgtcgtcga gatccggact tccgactgga
    19021 actgggacga ctacgaggtg agcattcatc tcgaagaacc ttgaacg
//
LOCUS       DAOOTK010000053        18794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2035_length_18794_cov_5.779284, whole genome shotgun sequence.
ACCESSION   DAOOTK010000053 DAOOTK010000000
VERSION     DAOOTK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18794
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2035_length_18794_cov_5.779284"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PK634_07665"
     CDS             complement(<1..1140)
                     /locus_tag="PK634_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97650.1"
                     /translation="MKRNLLWPRGLAVLLALRAAAGAFGQTPAAGEPTWDGLAQECPA
                     GLDPHRWQRLLDQFKADGLPPASAETCLGPAREAARDGLPAEAVMGRLEEGATKRAGA
                     EALAKAAEQRLQHLKTAGSLLREAGYGQRSPRHDELMMSVTLAMESGLSAPTLRGVLA
                     QGSGGQSERLRSIVEAGETMVLDGMDEPTVGLMMTDFTQRNMRRSEIMRATRFAVQQH
                     NARMEGSRIRQQLWNGAGSGGRWSEGGAGDGSGNGAGPGGGGGGYSSRGGGSSRAGGV
                     YGSPAAGSPGSAGSGGGPTTSSGSPGSGPGPGSSYSSPGTSPSSSGPSSSTPGNTSSD
                     AGSGSPGTGGSGSNQGGSGSNQGGSGSGQGGSGSGQGGSDNQGNSG"
     gene            complement(1144..1749)
                     /locus_tag="PK634_07670"
     CDS             complement(1144..1749)
                     /locus_tag="PK634_07670"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOW97651.1"
                     /translation="MKQDYHKRFHERLDGETIPPDATDAEEEARLTAYRAELGRLENR
                     RVRAPADLAARVMEAITPAPRPTFLEWLWTLVPTRRQWAIPALAGAVLMLALLPVLRQ
                     RSALSGPPRMLVHFQIHAPGAERVELVGDFNNWTAGTIQLQGPDASGHWTTDVELPEG
                     RYEYQFLVNGTTWVTDPGAEIRRPDGFGRENAVRVVYEERS"
     gene            complement(1746..2285)
                     /locus_tag="PK634_07675"
     CDS             complement(1746..2285)
                     /locus_tag="PK634_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW97652.1"
                     /translation="MNASDAEIVAQCREGDTNAFAELVRRNQDSVFNLVWSMTGNWHE
                     AADIAQETFIRAFRKLHSYKPEFSFRNWVMSIGANLTRNRFRSFSRRRRMQETLTRMQ
                     DADVAPAPGLPDEGLEAALGQMPETLRTALLLKHVEGQSYEEIARTLGIGLSAAKMRV
                     ARGRDELVHLLTTERERES"
     gene            2444..2707
                     /locus_tag="PK634_07680"
     CDS             2444..2707
                     /locus_tag="PK634_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97653.1"
                     /translation="MKTAVAVLAMATGILLAFDTAQAAGGNARQRKGNGYGTATRTGS
                     ASGTALQTRAQDRLRDGSCLTSATAATQDRLQSRLRDGSCLNK"
     gene            complement(2925..4055)
                     /locus_tag="PK634_07685"
     CDS             complement(2925..4055)
                     /locus_tag="PK634_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97654.1"
                     /translation="MKKLAVIFLGLLAALPAGARIKLVALPDRDTTMIRLDNPAATVV
                     EEERVLTLQKGLNQVDFSWKGVTIDQDSIRLAVLSPADKVQLLNVSYPPGEGALVWQL
                     SCAEACNAKVRISYLLSGIDRLVTYTGTAEKDESKLDLESFLVLRNFSGEDFIDAQVR
                     LDYGEPFSMSAKHEETRQRLFLSKTGLPIVKTFKWDAREKPWDPSRLDENVGIPVYYA
                     IANTEAKGLGENALWDGKVRVFQKDGHGSTIFVGEDNPGFVPVGEDLEIRVGEGRDVV
                     VTQRQKVNRQINIKRNSSNDIILYDNEELIETEVENFKDQPAVLVLTQYIPGEWTMRE
                     ASLDYELKDHETLEFRVELKPKDKITLKMDFVRSNIRTRDLR"
     gene            complement(4052..5395)
                     /locus_tag="PK634_07690"
     CDS             complement(4052..5395)
                     /locus_tag="PK634_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOW97655.1"
                     /translation="MKKWTALALAACGFVLPALAGIDLVTLPERDSVQLTIYNSADLT
                     LVREVRKLTLRKGLNKLSFGWANTLIDPTSLRLRAVDKPDAVKLLDVAYPPRINSQAV
                     WNVQSEVEGEVLVEITFFTSGISWRAFYMATLSQDEKDMLLEGYVRVTNNSGEDYANA
                     QTRMIVGVVNLIDEIADLARRKEPYGRPYPEVVMAAPMALRMSRAPNAMALAGAMDYA
                     EEASPKEIVKEGLSEYFLYTIPGTETIPNQWSKRLPSFKPVRVPVVNLFRYDEERYGS
                     TTMRLLFFKNDEKHHLGETPLPDGFFKVYRTADQQDHLSYEGATPIKYIPVEQEAELN
                     LGAARGVKVEPKLMKIQTDQYLFNGTNGNISGYDQAEDWTLELNNYRDIPVRVEVFRR
                     LRHPYVDEIKPKGELASFEKQDAQRVKFVVELSPNTEREITYRVVYHEGDRRERR"
     gene            complement(5472..7163)
                     /locus_tag="PK634_07695"
     CDS             complement(5472..7163)
                     /locus_tag="PK634_07695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW97656.1"
                     /translation="MKELLKKTAWLWPLLAAAALLALQVPWTETLTYQRYDEGYTGCL
                     MTSRALGFRLHDDLWIDQPPLFFTLLFAWFHWFGASLLKGRALVFLLSVLFSGLFFDT
                     IRRRSTPAAAWLALVFLLTRPVYAMMSVTMIQELPAYTFAGLALWLATVRTGHKRPGL
                     LLALSAVAMGCALLVKLSAAPFVPLIAFDLFCRPRQPAARPSSRLLSIAAWGLIAGVS
                     FAAIGWLMGADFRELVQAHLNPMETPILSLRSGTRMLPVFVFANWIPMLLAAPSFVYG
                     LRLREYSFGPPLLWLFLNAVLLNFYKPVADHYEIHVIVPLCWLAGATLARAASGRWLA
                     LAGTVALAAIPGNRFILRAPNWQVYPGTLAAVREYAPRTRWMAAIECQIYPFAAGVNV
                     IPDLYSTSLKRMLSGRMSSENTLQQIKQYRPEQVMMDNAFGYSPELAAYLAAHYRCRL
                     PDNAYAQWVRSDVADPAGGWLADPFRDMALGRNEARLKPFSLALHFLKLNQPPNARLI
                     LEQALELRTPALAPHRRDAWGLLGDILQREGREDEARECFQKARAPLSSGGAPAP"
     gene            complement(7160..8137)
                     /locus_tag="PK634_07700"
     CDS             complement(7160..8137)
                     /locus_tag="PK634_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97657.1"
                     /translation="MRPKILYQSLILLCLAAALLEMSLTLVSPERRGEWNGRFDDQIY
                     TWGHPVRPNLQGLREREIAVPKPAGVFRVLVLGDESTWGPGLAEDERFTALAESRLRQ
                     QDPDRRIEVLNAGLPEATLPVLRDALLFWCEAPVEPDLVVVAFGGGALRLDSNENRPE
                     HVAFARRHPAIGLAAPRALTALRMPQTARLWRRTAARLAEATGAAPSYEEFLAHRYGP
                     DSPAWPEFAGALRDIREFCRARNLPPPVFLSLHVGHPAADWYARAAKQAEESGFVAPS
                     CVADIAPKVDARDINLLNGIHPRHFQAAWAESLSRSVAAALDRQHEVLP"
     gene            complement(8224..10473)
                     /locus_tag="PK634_07705"
     CDS             complement(8224..10473)
                     /locus_tag="PK634_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOW97658.1"
                     /translation="MALDPICGMTVDERTALRTEVDGTVSYFCSNHCRLKHLGHHSPS
                     AIHHSPFFCPMCPGVESDRPGTCPTCGMALEASPTAQADGGEFRLMQRRLAIGLALGL
                     PVFLLGMTHLLPDGLAARIPHRVSAWIQLLLGTPVIFWCGAPLIARGWLSFRTLRLNM
                     FSLILLGVMAAYLFSLAALLTPGLFPDSLLHDGHVPVYFESAAMITVLVLLGQVLELR
                     ARHRTGSALREWMALAPETARRMRDGEEQVVALDRVRPGDILRVKPGDRVPVDGEVIE
                     GRSHVDESMLTGEPLPVEKETGGKVSGGTLNGSGSFLMRAEKVGAETLLARIVQLVSE
                     AQRSRAPIQRLADRVAAWFVPAVIAISALTFLLWWRLGPEPRLEHALVNAVAVLIIAC
                     PCALGLATPMSMTVAVGRGARAGVLIRNARAIEMLGRLRTLVVDKTGTLTEGRPRLTA
                     CVPVPPLTENELLRRAAAVERHSEHPLGEALVAAAREQGLDLPEPSDFQAVPGKGAAG
                     RVGGQPVLVGHSAFLEERDVTGLDTFRALAERLQNDGQTTMLVAADGQLAGLLGVSDP
                     IKDSTPAALDALRARGLRLVIVTGDQERTARAVARRLGIEEVHAGVRPEGKHAFLEQL
                     PPRDRPVAMAGDGINDAPALAAADVGIAMGTGTDIAVQSADVTLLHGDLRGIARAVVL
                     GRAAMRNIRQNLFFAFLYNSLGIPLAAGALYPFFGLLLSPVLAGAAMSFSSVSVVLNA
                     LRLRRTRLD"
     gene            10642..10738
                     /locus_tag="PK634_07710"
     tRNA            10642..10738
                     /locus_tag="PK634_07710"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10677..10679,aa:Sec,seq:tca)
     gene            10803..12596
                     /locus_tag="PK634_07715"
     CDS             10803..12596
                     /locus_tag="PK634_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOW97659.1"
                     /translation="MFHPGKAASWIVGGSLLLAGVAPAARAGEPVDILVLESFGRDVS
                     PWDSTTAAFKKELVRLSPAPVNFHEVTVGVGGLVNPFGDAALVDYLGDLYSRRKPALA
                     VLMAAPAAAFWGRQGEKMFPATPAVIGGIEHRQLPRIKVGSNTVAVVVDFDIAGRLAA
                     VSEVLPEATNIVVVVGDSLLERFWQAECRRAWAPYTNRFQFTWLSGLPFPEICRRVAA
                     LPPRTVIGYGILCVDAAGVSHEKLDALDEIVRVANAPVLGMFEEQLGRGILGGRLFSG
                     EELGLETARVAARVLAGDKPGSLSPPMIRSGPPMFDSRQLRRWKVDERLLPPDSVVRF
                     RQPTLWAQYRWIIAGTLTILLLQTATIAGLLVQRVWRRRAEKAAREVSGRLITAQEEE
                     RRRIARDLHDDLSQRLALLSVELEMAGQSSGPDPAAPGDALGEMAAQVQEISADVHKL
                     SYQLHPAKLDQLGLVTTARTLCHELSVQSGLGIGFSHQNVPRDLPAEVALCVYRVLQE
                     SLGNAIRHSGATGVSAALRMEEGFIRLEVSDNGKGFDSGRARREGGLGLLSMEERARL
                     VRGRLDIHSRPGHGARVELSVPVAAEEEGGA"
     gene            12593..13261
                     /locus_tag="PK634_07720"
     CDS             12593..13261
                     /locus_tag="PK634_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW97660.1"
                     /translation="MSRPRVLLADDHGLLLEALRKLLEPACDVVGTVTDGRALVETAL
                     ELKPDVIVADIGMPRLNGLDAGAQLQARLPAAKMIFLTVNEDPDIAAEAVRRGARGYL
                     LKKSAATELFAAIQQVHAGRSYITPLITREPPEVFVARAQLGRSAPGLSLRQREVLQL
                     LAEGCSMKEAAGILKVTTRTIAFHKYAMMKQHGFRTGAELVQYAVRLGLVADRGGLPP
                     SCQF"
     gene            13313..13750
                     /locus_tag="PK634_07725"
     CDS             13313..13750
                     /locus_tag="PK634_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW97661.1"
                     /translation="MENAQKPARLLIVDDHPPLARQVEQLLRGEFDVVGTLTSGAALD
                     TAVAELHPELIVMDITLPGASGIELARRLTARGCPARIVFLTVHADADYARESMTAGG
                     LGYVIKPRLGSDLAPALRAAREGRSFVSPCPELVGLQGPGSST"
     gene            13795..15468
                     /locus_tag="PK634_07730"
     CDS             13795..15468
                     /locus_tag="PK634_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOW97662.1"
                     /translation="MSNRDVAKQCAAGLGRVGTWAMVGSVLSAGSLVSPASAQSTESA
                     PAGKDGGHPNILVIFGDDIGQANISVFTKGLMGYQTPNIDRIAKEGMLFTDYYAEQSC
                     TAGRSSFITGQCVFRTGLSRVGVPASPIGIRAEDPTIAELIKPLGYATGQFGKNHLGD
                     RNEHLPTVHGFDEFYGNLYHLNAEEEPENYNYPQDPKFRMMFGPRGVLRCKATDVDDP
                     TVDPRFGKIGKQTVEDTGPLTRKRMETIDDETSEAAIDFMQRQVKAGQPFFCWYNSTR
                     MHFRTHVRPEHRSPRGAKARTEYADGMVEHDGTVGSLLKALDDLGIADNTIVIYTTDN
                     GPHANSWPDGATTPFRSEKNTGWEGGFRVPCFIRWPGRIEADRISNEIVSGLDWLPTL
                     LAAAGNPNIKEQLLAGYSAGDRQFRVHLDGYNLLPYLTGQAAESPRREFFYFSDDGDL
                     LNLRYENWKIVFAEQRCTGTMAVWAEPFTPLRLPKFFDLRADPYERADVTSNTYYDWY
                     LDHMYFTIPAAQYALKFIATFKEFPPRQKPPGSEIEKAVDRLLQAAAGR"
     gene            15482..16498
                     /locus_tag="PK634_07735"
     CDS             15482..16498
                     /locus_tag="PK634_07735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOW97663.1"
                     /translation="MNKRVFLGLMGILAAAAWAAAADPLPSWNDGAAKEAILQFVAGT
                     TTEGSPDFVPPAERIAVFDNDGTLWAEQPMYFQGLFMLDRVKRMASQHPEWKEQEPFA
                     SLLKGDLKTAFAGGEAAVGRLLMTTHAGMTSEEFEKMAADWIAGARHPKTGRLLTEMV
                     YQPMLELLAFLRANGFKTFIVSGGGMEFMRPWAERVYGIPPEQVIGSSIKTKFELRDG
                     VPVLVRMPESDFVDDGPGKPVGIQMHIGRRPVAAFGNSDGDLAMLQWTAAGPGARLCL
                     IVHHTDADREWAYDRESHAGRLDKGLDEAAARGWTVVDMKNDWKRVFVFEAEQLVRPD
                     SGPQ"
     gene            16511..>18794
                     /locus_tag="PK634_07740"
     CDS             16511..>18794
                     /locus_tag="PK634_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOW97664.1"
                     /translation="MMRENHGIRQAGRWAAVGSVLATGALFGARATGQEVLPTPPMPF
                     KGQIGLSAADSTPDFPKPVKAPPGAPNVVLVLLDDVGFGASSTFGGPCQTPTFDRLAK
                     SGLRFTQFHTTALSSPTRAALLTGRNHHSVHTGVIMEQGTGYPGYDSLMGGDTATIAE
                     ILKQKGWNTSWFGKNHNVPDWQSSQAGPFDLWPIGLGFEHFYGFVCAETSQWRPAVTE
                     GTTPIEPYLGNPDYHFDYDIADRAIEWVRNQKAVAPDRPFFLYYAPGATHSPHHAKPE
                     WIAKYKGRFDRGWDAVREETLAKQKELGLVPADTQLTPRPERIPAWDTLNAQQKDLYA
                     YMMEVYAGFLEQTDYNVGRVLEGIGKMGQLDNTLVIYICGDNGASAEGMDNGELNELA
                     GLNGVDEDYAEVLKHKDDLGTWKTHNHYPAGWAHAMCSPFQWAKQIASHYGGTRNGMV
                     ISWPARIKDAGGIRPQWHHVIDVVPTVLEACGLQAPSIVNGVAQKPIEGVSMAYTFDN
                     AQAPTARRTQYFEMFGNRAIYHDGWVACTTPRAAPWQDTSRLPPVDIISGYRWELYHV
                     AEDFSEAVNLADQNPDKLRELQLLFYAEAAKYNVLPLDDSKIERLNPAIRPSLTRGRT
                     EFTYYGPVRRIPEGAAPDVKNKSWSVAADVVLATGAEQGVLVTQGGLFGGYALMLRDG
                     KPVFHYNMLNVAHFNIAATTALAPGKHTIAFEYQHDSGVGLGRGGNGILSVDGQPVAQ
                     GRIERTIPLRFTLDETFDIGE"
BASE COUNT         3113 a         6256 c         6487 g         2938 t
ORIGIN      
        1 cccgctgttg ccctgattgt ccgatccgcc ctggccgctg ccggatccgc cctggccgct
       61 gccggatccg ccctggttac tgccggatcc gccctggtta ctgccggatc cgccggtgcc
      121 cgggctcccg ctgccggcat ccgaggatgt attacccggt gtggaggaag aggggccgga
      181 tgaggaaggt gatgtgccgg gcgacgaata ggaactgccg ggtcccggcc cgctgcccgg
      241 gctgccgctg ctcgtggtcg gcccgccgcc gcttcccgcg ctgccgggag atcctgccgc
      301 cggggaaccg tacaccccgc cggcgcggct ggagccgccg ccccggctcg aatacccgcc
      361 cccacctccg ccgggtccgg ccccgttgcc tgatccgtct cccgcgcctc cttcggacca
      421 tcggccgccg ctcccggcgc cgttccacag ttgctggcgg atacgggagc cctccattcg
      481 cgcgttgtgc tgctgcaccg cgaaacgggt cgcccgcatg atttccgagc ggcgcatgtt
      541 gcgctgggtg aagtccgtca tcatcagtcc gaccgtcggt tcatccatcc cgtcgagaac
      601 catcgtctcg cccgcctcca cgatgctgcg caggcgttcg ctctggccgc ccgagccctg
      661 ggccagaacg cctcgcagcg tgggcgcgga cagcccgctt tccatcgcca gggtcacgga
      721 catcatcaac tcgtcgtgcc gcggcgagcg ctgaccgtag ccggcttcgc gcaacaagct
      781 gcccgcggtc ttcaggtgct ggaggcgctg ctcggcggcc ttcgccaggg cctcggctcc
      841 cgcacgtttc gtggcgcctt cctcaagccg gcccatgacg gcctccgcgg ggaggccatc
      901 ccgcgccgcc tctctcgcgg ggccgagaca cgtctctgcc gacgcggggg gcaggccgtc
      961 ggccttgaac tggtcaagca gccgctgcca tcggtgcggg tccagcccgg ctggacactc
     1021 ctgcgccagg ccgtcccacg tcggctcgcc ggcggcgggc gtctggccga aggcaccggc
     1081 tgcggcccgc agcgcgagca gcacggccag gccccggggc catagcaggt tgcgcttcat
     1141 cgcctaactc ctttcctcgt agaccacgcg gacggcattc tcccggccga aaccgtccgg
     1201 ccgtcggatc tccgcgcccg ggtccgtcac ccaggtggtg ccgttgacga gaaactggta
     1261 ctcgtagcgc ccctcgggca attcgacgtc cgtcgtccag tgccccgagg cgtccgggcc
     1321 ctggagttgg atcgtgccgg ccgtccagtt attgaaatcc ccgacgagtt ccacgcgctc
     1381 cgcccctggc gcgtggatct ggaagtgcac cagcatccgc ggcggtccgg acagcgcgct
     1441 tcgctgccgc agcaccggca ggagagcgag catgagcacg gcgccggcca gcgcgggaat
     1501 cgcccactgc cgccgcgtcg ggaccagcgt ccaaagccat tccaggaaag tcgggcgcgg
     1561 ggccggcgtg atggcctcca tgacgcgcgc ggcgaggtcg gcgggcgcgc ggacccgtcg
     1621 gttttcgagc cggccgagct ccgcgcgata cgccgtcagc cgggcttcct cttccgcgtc
     1681 cgtcgcgtcc ggggggatgg tctcgccgtc cagccgctcg tgaaaccgct tgtggtaatc
     1741 ctgtttcatg actcgcgctc ccgctcggtt gtgagcaaat ggaccagttc gtcgcgcccg
     1801 cgggccacgc gcatcttggc cgcgctcaac ccaatgccca gtgtgcgcgc gatttcctcg
     1861 taggactggc cctcgacgtg tttgagcagc agcgccgtgc gcagcgtctc gggcatctgg
     1921 cccaaggcgg cctccaggcc ctcgtcgggc aatcccggcg ccggcgcgac atcggcatcc
     1981 tgcatgcggg tcagcgtctc ctgcatccgc cgccgccggc tgaagctccg gaaccggttg
     2041 cgcgtgagat tggcgccgat gctcatgacc cagttccgaa aagaaaactc gggcttgtac
     2101 gaatgcagtt tccggaaggc ccggatgaag gtctcctgcg cgatatcggc cgcctcatgc
     2161 cagttgccgg tcatgctcca caccaggttg aatacggagt cctgatttcg ccgcaccagt
     2221 tccgcgaagg cgttcgtgtc gccttcccgg cactgggcga cgatctcggc atcgcttgca
     2281 ttcattatct cctgcaactt gtacaccgga ggactctgtt ttgtcacgtg gcgcctctga
     2341 aaaaccgtaa tgtgaccttt ccaggagctt cggtgtatcc ccagtagaac atccggcggt
     2401 tcggatggga cgaaataggg caacagcccg gaaggagcct tgaatgaaga cggccgtagc
     2461 ggttctggcg atggcgacgg ggattttgct ggcgttcgac acggcgcagg cggcgggcgg
     2521 aaacgcccgg cagcgcaagg gcaacgggta tgggaccgcc acccgcacgg gcagcgcttc
     2581 cgggacggcg ctccagacgc gcgcgcagga tcggctgcgc gacggctcct gcctgacctc
     2641 cgccaccgcc gcgacccagg atcgcctgca gagccgtttg cgcgatggtt cgtgcctgaa
     2701 caagtaacac aaggaaagga gcggagacca tgaagaagtg gatgatcgtt ctgctgctgg
     2761 tgctcggggc ggtttcggtg ttcgccggcg gggataaaaa ccgcggcgac aagggcaagg
     2821 gcgccgttca ccaggtcgtg ggaccataaa tcgcgccgcc cgcggggggg cacagttaat
     2881 taattgtgcc ccctccgggg gaacggggct aggccttcaa ggacttaccg cagatcccgc
     2941 gtgcggatgt tgctccggac gaagtccatc ttgagcgtga tcttgtcctt gggcttgagc
     3001 tcgacccgga actcgagcgt ctcgtggtcc ttcaactcgt agtcgaggct ggcctcccgc
     3061 atcgtccact cgcccgggat gtactgggtc agcaccagca ccgcgggctg gtccttgaag
     3121 ttctccacct cagtctcgat aagctcctcg ttgtcgtaga ggatgatgtc gttcgacgag
     3181 ttgcgcttga tgttgatctg gcggttgacc ttctggcgct gggtgacgac cacgtcgcgg
     3241 ccttcgccga cccggatctc caggtcctcg ccgaccggca cgaagccggg attgtcctcg
     3301 ccgacgaaga tcgtcgagcc gtgcccgtcc ttctggaaga cgcgcacctt tccgtcccag
     3361 agggcgttct ccccgaggcc cttcgcctcc gtgttggcga tcgcgtagta gaccgggatg
     3421 cccacgttct cgtcgaggcg cgacgggtcc cacggcttct cgcgggcgtc ccacttgaac
     3481 gtcttcacga tcggcaggcc ggtcttgctc aagaacagcc gctgccgcgt ctcctcgtgc
     3541 ttcgcgctca tcgagaacgg ctcgccgtag tccaggcgaa cctgggcgtc gatgaagtcc
     3601 tcgcccgaga agttgcgcag caccaggaac gactccaggt ccagcttgct ctcgtccttc
     3661 tcggccgtgc cggtgtacgt caccagccgg tcgatcccgg agagcaggta gctgatgcgc
     3721 accttcgcgt tgcaggcctc cgcgcaggag agctgccaca ccagcgcgcc ctcgccgggc
     3781 gggtagctga cgttcagcag ttgtaccttg tccgcggggg agagcaccgc caggcggatg
     3841 gagtcctggt cgatcgtgac gcccttccac gagaagtcca cctggttcag gcccttctgg
     3901 agcgtcagca cgcgctcctc ctcgaccacc gtggcggccg gattgtccag gcggatcatc
     3961 gtcgtgtcgc ggtcgggcag ggcgacgagc ttgatgcggg cgccggcggg cagggccgcc
     4021 agcaggccca ggaaaatgac ggcgagcttt ttcatcggcg ttccctcctg tctccttcgt
     4081 gatagacaac ccgataggtg atttcgcgct ccgtgttcgg cgacagctcg accacgaatt
     4141 tcacccgctg ggcgtcctgc ttctcgaacg aggccagttc gcccttaggc ttgatctcgt
     4201 ccacgtacgg gtgccgtagc cggcggaaga cttccacgcg gacggggatg tcgcggtagt
     4261 tgttcagttc cagcgtccag tcctcggcct ggtcgtagcc gctgatgttg ccgttcgtgc
     4321 cgttgaacag gtactggtcg gtctggatct tcatcagctt cggctcgacc ttcacgccgc
     4381 gcgccgcgcc gaggttcagt tccgcctcct gctccacggg gatgtacttg atcggtgtcg
     4441 cgccctcgta ggagaggtgg tcctgctggt ccgccgtccg gtagaccttg aagaacccgt
     4501 ccggcagcgg cgtctcgccc aggtggtgct tctcgtcgtt cttgaagaac agcaggcgca
     4561 tcgtggtcga gccgtagcgc tcctcgtcgt accggaacag gttcaccacc ggcacgcgca
     4621 cgggcttgaa ggacggcagg cgcttggacc actggttcgg gatcgtctcc gtcccgggga
     4681 tcgtgtagag gaaatactcc gacaggcctt ccttcacgat ctccttcggg ctggcctctt
     4741 cggcgtagtc cattgcgccc gccaaggcca tcgcgttcgg cgcccgggac atcctcaagg
     4801 ccatgggagc cgccatcaca acctccgggt atggccggcc gtacggttcc ttgcgccgcg
     4861 ccaggtccgc gatctcgtcg atcaggttga ccacgccgac gatcatgcgg gtctgggcgt
     4921 tggcgtagtc ctcgccggag ttgttggtca cgcggacgta gccttcgagg agcatgtcct
     4981 tctcgtcctg cgagagcgtg gccatgtaga acgcgcgcca ggagatgccg gaggtgaaga
     5041 acgtgatctc gacgagcacc tcgccctcga cctcggactg gacgttccag accgcctgcg
     5101 agttgatccg cggcgggtag gcgacgtcga gcagtttcac cgcgtcgggc ttgtccacgg
     5161 cgcgcaggcg cagggaggtg ggatcgatca gcgtgttggc ccagccgaag gagagcttgt
     5221 tgagcccctt ccgcagggtc agcttgcgca cctcgcggac gagcgtgagg tccgcggagt
     5281 tgtagatggt gagctgcacg ctgtcgcgct ccggcagcgt gacaaggtca atgccggcga
     5341 gagcgggcag aacgaatccg catgccgcga gcgccagcgc cgtccatttc ttcataggca
     5401 cgttcctcct gttgatggcg gtatcatacc agccgccggg gccttgtcga tgatccctcg
     5461 gctcttgact actacggcgc gggcgcgccg ccggatgaca gcggggcgcg ggctttctgg
     5521 aagcactccc gggcctcgtc ctcccgccct tcccgctgca ggatgtcgcc gaggaggccc
     5581 caggcgtcgc gccggtgcgg cgcgagggcg ggggtgcgga gttccagcgc ctgctccagg
     5641 atcagccgcg cattcggcgg ctggttcagc ttgaggaagt gcagggcgag cgaaaagggc
     5701 ttgagccggg cctcgttccg gcccagcgcc atgtcccgga aaggatcggc cagccagccg
     5761 ccggccggat cggccacgtc gctgcggacc cattgagcat aggcgttgtc ggggagacgg
     5821 caccggtaat gcgccgcgag gtaggcggcc agttcgggcg aatagccgaa ggcgttgtcc
     5881 atcatcacct gctccgggcg atattgcttg atttgctgca gcgtgttctc ggaactcatc
     5941 cgcccgctta acatcctctt cagtgacgtg ctgtagagat cggggatcac gttcacgccc
     6001 gcggcaaagg gatagatctg gcattcgata gccgccatcc agcgggtgcg cggggcatat
     6061 tcccgcaccg cggcgagcgt gccgggatac acctgccagt tcggggctcg caaaatgaaa
     6121 cggttgcccg ggatcgcggc cagcgcgacg gtcccggcca acgccagcca gcggcccgat
     6181 gccgcgcgcg ccagggtggc ccccgccagc caacacaggg ggacgatcac gtggatttca
     6241 taatgatcag ccaccggctt gtaaaagttg aggagcacgg cgttgaggaa aagccagagc
     6301 aacggcggcc cgaacgagta ctcgcgaagg cgcagcccat acacgaatga cggagcggcc
     6361 aggagcatcg ggatccagtt ggcgaacacg aataccggca gcatccgcgt cccgctccgc
     6421 aaggaaagga tgggcgtttc catcggatta agatgtgcct ggaccagttc gcggaagtcg
     6481 gcgcccatca gccagccgat cgccgcgaaa gacacgccgg cgatcaggcc ccaggccgcg
     6541 atcgacagga gccgcgacga cggccgcgcg gccggttgcc ggggccggca gaagaggtcg
     6601 aacgcgatca gcgggacgaa cggcgccgcg gacagcttga cgagcagcgc gcagcccatg
     6661 gcgaccgctg ataatgccag cagcaggccc ggccgcttgt gcccggtacg gaccgtggcc
     6721 agccagagcg ccaggcctgc gaaggtatag gccggcagct cctggatcat cgtcacgctc
     6781 atcatggcgt acacgggacg ggtgagcagg aagaccagcg ccagccaggc cgcggccgga
     6841 gtggagcgcc ggcggatggt gtcgaagaaa aggcccgaga agaggacgga taacaggaaa
     6901 accagcgcgc gccccttgag cagggatgcc ccgaaccagt gaaaccacgc aaaaagaagg
     6961 gtgaaaaaga gggggggctg gtcaatccat aagtcgtcgt gcagccggaa gcccagtgcc
     7021 cgcgaggtca tcaggcatcc ggtgtaaccc tcgtcatagc gctggtacgt cagcgtctcg
     7081 gtccacggca cttgcagcgc gagcagggcg gccgccgcga ggaggggcca cagccaggcc
     7141 gttttcttca gtagctcttt cacggcagga cctcgtgctg gcggtcgaga gcggccgcga
     7201 cgctcctgga caggctctcc gcccaggccg cctggaagtg gcgggggtgt atgccgttca
     7261 gcaggttgat gtcccgggcg tcgactttcg gcgcgatgtc ggcgacgcag gagggcgcaa
     7321 caaagccgga ctcctccgcc tgtttcgcgg cccgggcgta ccaatccgcc gccgggtggc
     7381 ccacgtgcag actcaagaag acgggcggag gcagatttcg cgcccggcag aactcacgga
     7441 tatcccgcaa tgcgccggcg aactccggcc acgccggcga atccggaccg tacctgtgcg
     7501 cgaggaactc ctcgtacgaa ggcgccgcgc ccgtcgcttc cgccaggcgc gccgccgtcc
     7561 ggcgccacag gcgggcggtc tgcggcatcc ggagcgcggt cagggccctg ggcgcggcca
     7621 ggccgatcgc cgggtgccgc ctcgcgaacg cgacgtgctc gggccggttc tcgttcgagt
     7681 ccagccgcag ggcgccgccg ccgaacgcca cgaccacgag gtccggctcc accggggcct
     7741 cgcaccagaa cagcagcgcg tcgcgcagga ccggcagcgt ggcctcggga agcccggcat
     7801 tcagcacctc gatacgacgg tccggatcct gctggcgcaa ccgcgattcg gccagggccg
     7861 tgaatcgctc gtcctcggcc aggcccgggc cccacgtgga ctcatcgccg aggaccagca
     7921 cgcggaaaac gccggcgggc ttgggcacgg cgatctcgcg ttcgcgcaga ccctggaggt
     7981 tggggcgcac gggatggccc catgtataga tctggtcatc gaagcggcca ttccactcgc
     8041 cgcggcgctc cggggacacc agcgtcagcg acatctccag cagggccgcc gcgaggcaaa
     8101 ggaggatcag cgattggtag agaatttttg gccgcatcgg gtttccaatc attggaaaat
     8161 tccgggcggt atttttccaa tgattggaag gaatggcacg cactatttcc aatgattgga
     8221 aaactaatcc agacgggtcc gccgcaggcg cagggcgttg aggacgacgg agacagagct
     8281 gaagctcatg gccgcgccgg ccaggacggg gctcaacagg aggccgaaga agggatacag
     8341 cgccccggcg gcgagcggga tgcccaggga gttgtacagg aacgcgaaga agagattctg
     8401 ccggatgttc cgcatcgcgg cccggccgag gaccacggcg cgcgcaatgc cccgcaggtc
     8461 gccgtgcagc agggtgacgt ccgcgctctg cacggcgatg tccgttccgg tgcccatcgc
     8521 gatcccgacg tcggcggcgg cgagcgccgg cgcgtcgttg atcccgtcgc cggccatcgc
     8581 gacgggccgg tcccgcggcg gaagctgctc cagaaaggca tgcttgccct ccggccggac
     8641 gccggcatgc acctcctcga ttcccagccg gcgggcgacg gctcgcgccg tacgctcctg
     8701 gtcgccggtg acgatgacca gccgcaggcc gcgcgcgcga agggcgtcca aggcggcagg
     8761 ggtggagtcc ttgatcggat ccgatacgcc cagcagcccg gccagttggc cgtccgcggc
     8821 gacgagcatg gtcgtctgcc catcgttttg caggcgctcg gccagggccc ggaaagtatc
     8881 caggccggtg acgtcgcgtt cctccaggaa ggcggaatga ccgacgagga ccggctggcc
     8941 gccgacccgt cccgccgcgc cttttcccgg aacggcctgg aagtccgaag gttcggggag
     9001 gtcgagaccc tgttcccggg cggcggccac cagggcttcg cccagcgggt gctcgctgtg
     9061 gcgctccacg gcggccgcgc gccggagcag ctcgttctcc gtcagcggag ggaccgggac
     9121 acaagcggtc aggcggggcc ggccctcggt cagtgtgccg gtcttgtcca cgaccagcgt
     9181 gcggagccgg cccagcattt cgatggcccg ggcgttgcgg atcaacacgc cggcgcgcgc
     9241 gccgcgcccg acggcgacgg tcatgctcat gggcgtcgcg aggccgagcg cgcaggggca
     9301 ggcgatgatc agcacggcca cggcattgac cagggcgtgc tccaggcggg gctccggccc
     9361 gagccgccac cacaggagga acgtcagcgc cgagatggcg atgacggcgg ggacgaacca
     9421 ggccgccacg cggtcggcga gccgctggat cggcgcgcgg ctgcgctggg cctccgacac
     9481 gagttgcacg atgcgcgcca ggagcgtctc cgcgccgacc ttttccgcgc gcatcaggaa
     9541 cgatccgctc ccgttcagcg tgccgcccga taccttgccg ccggtttcct tctccaccgg
     9601 cagcggttct cccgtcagca tggattcgtc cacgtggctc cggccctcga tcacttcgcc
     9661 gtccaccggg acccggtcgc ccggcttcac gcgcaggatg tccccgggcc gaacccggtc
     9721 cagggcgacg acctgttcct ctccgtcgcg catccgccgc gccgtttcgg gcgcgagggc
     9781 catccattcg cgaagggcgc ttccggtgcg atgccgcgcc cgcagttcca ggacctggcc
     9841 gagcaggacc aggaccgtga tcatggccgc ggactcgaaa tagaccggga catggccgtc
     9901 gtggagaagg gaatccggga agagcccggg cgtgagcagg gcggccaggc tgaagagata
     9961 agccgccatc acgccgagca ggatcaggga gaacatgttg agccgaagcg ttcggaacga
    10021 cagccatccc cgtgcgatca gcggcgcccc gcaccagaag atcaccggcg tgccgagcag
    10081 gagttgaatc caggcggaga cgcggtgggg gatgcgggcc gccaggccgt ccgggagcag
    10141 gtgcgtcatg ccgaggagaa agaccgggag cccgagggcc agcccgatcg ccagccgccg
    10201 ctgcatcaac cggaattcgc cgccgtccgc ctgcgctgtc ggcgacgctt caagggccat
    10261 gccgcaggtc gggcacgtcc cgggccggtc gctttccacg cccgggcaca tggggcagaa
    10321 gaaaggtgaa tggtgaatgg cggatggtga atggtggccg aggtgcttca agcggcagtg
    10381 atttgagcaa aagtagctga ccgtgccgtc cacttccgtc cggagcgcgg tgcgttcgtc
    10441 cacggtcatg ccgcatatgg gatccaaggc catgatgcag tatacatcat ttcttcatcc
    10501 cgctcctact ccttctccct cttcatccgc tcgcgctcaa tagccaaacg ggcaggtcgc
    10561 gatgtggaga aggagaaaga gtgggaggag taggtgttgg acgggagggg cgtgaatttg
    10621 gtagaccatg cgtggcggga ggggagtgac aaggtacctg gtgggcctcg cggacttcaa
    10681 atccgttgtg gggctttaac cggccccggt tgggttcgat tcccactcac tcccgccatt
    10741 gccccggctc cccgggcgac ttggggattg aacgagaacg gccgccgggc ataatcgagt
    10801 tcatgtttca cccgggcaag gcggcgtcgt ggatcgtcgg cgggagcctg ctgctggcgg
    10861 gcgtcgcgcc ggcggctcgg gcgggcgagc ccgtggatat cctggtcctg gaatccttcg
    10921 gccgcgacgt ctcgccctgg gattccacca cggccgcctt caagaaggaa ctggtccggc
    10981 tgtcgccggc ccccgtcaac ttccacgagg tcaccgtcgg cgtcggcggg ctggtcaatc
    11041 cgtttggcga tgccgccctc gtggactacc tcggcgacct ctactcgcgg cgcaagccgg
    11101 cgctggcggt cctgatggcc gcgccggcgg ccgccttctg ggggcggcag ggcgagaaaa
    11161 tgttccccgc cacgccggcg gtcatcggcg gaatcgagca tcgccagttg ccgaggatca
    11221 aggtcggatc caacaccgtg gcggtcgtcg tcgatttcga catcgcgggg cgactggccg
    11281 cggtttccga ggtgctgccg gaggccacga acatcgtggt ggtggtgggc gactcgctgc
    11341 tggagcggtt ctggcaggcg gaatgccggc gggcgtgggc gccctatacc aatcgcttcc
    11401 aattcacgtg gttgagcgga ctgccgttcc cggaaatctg ccgccgcgtg gccgcgctgc
    11461 cgccgcggac ggtcatcggc tacgggatcc tctgcgtgga cgccgccggc gtgtcgcacg
    11521 aaaagctgga cgcgctggat gaaatcgtca gggtggccaa cgcgcccgtg ctgggcatgt
    11581 tcgaggagca actcggccgg ggcatcctgg gcgggcggtt gttttccggg gaggaactcg
    11641 gcctcgagac cgcccgggtg gccgcccgcg tgctggcggg agacaagccg ggtagccttt
    11701 cgccgccgat gatccggagc gggccgccca tgttcgattc ccgtcagctc cgccgctgga
    11761 aggtggacga gcgcctcctg ccgccggaca gcgtcgtgcg gttccgacag ccgacgctgt
    11821 gggcgcagta ccggtggatc atcgcgggca cgctgacgat cctgctgctg cagacggcca
    11881 ccatcgcggg actcctcgtg cagcgggtgt ggcgacgccg cgcggaaaag gccgcgcgcg
    11941 aggtcagcgg ccggctcatc acggcccagg aggaggaacg gcgccgtatc gcccgagatc
    12001 tccacgacga cttgagccag cggctggcct tgttgtcggt cgagttggaa atggccgggc
    12061 aatcctcggg ccccgatccg gcggcgcccg gcgatgcgct gggagaaatg gccgcccagg
    12121 ttcaggagat ctccgccgac gtccataagc tctcctacca gctccacccg gcgaagctgg
    12181 accagctggg gctggtgacc accgcgcgca cgctctgcca cgagctgtcg gtccagtcgg
    12241 gcctgggcat tgggttcagc catcagaacg tgccgcggga cctgccggcg gaggtcgccc
    12301 tgtgcgtcta ccgcgtgctg caagagtcgc tcggaaacgc catccgccac agcggcgcga
    12361 ccggggtctc cgccgccttg cggatggagg aggggttcat ccggctcgag gtttccgaca
    12421 acggcaaggg gttcgacagc gggcgggcgc ggcgcgaggg ggggctggga ctgttgagca
    12481 tggaggagcg ggcccggctg gtgcgcggcc ggctcgacat ccattcgcgc ccggggcacg
    12541 gcgcccgggt ggaactgtcg gtgccggtcg cggcggagga ggaggggggg gcatgagccg
    12601 gccccgggtg ctgctggcgg acgaccacgg gctgttgctg gaggccctcc gcaagttgct
    12661 ggagccggcg tgcgacgtcg tcggcacggt tacggacggc cgggcgctgg tcgagaccgc
    12721 gttggagctg aagccggacg tcatcgtcgc ggatatcggc atgccgcggc tcaacggcct
    12781 ggacgcgggg gcgcaattgc aggccaggct gcccgccgcg aaaatgatct tcctgacggt
    12841 gaacgaggat ccggacatcg cggcggaggc cgtgcgccgc ggcgcgcgcg gctacctgct
    12901 gaagaaatcc gcggcgaccg aactgttcgc ggccatccag caggtccacg ccggccgctc
    12961 gtacatcacc ccgctgatca cgcgcgagcc gccggaggtc ttcgtggccc gcgcgcagct
    13021 cgggcggagc gcgccgggct tgagcttgag gcagcgggaa gtgctgcagc tcctggccga
    13081 aggctgctcg atgaaggaag cggccggcat cctgaaggtc accacccgga cgatcgcgtt
    13141 ccacaagtac gccatgatga agcagcacgg gttccggacc ggcgccgagc tggtccaata
    13201 cgccgtgcgg ctcggcctgg tcgccgaccg cggcggcctg ccgccctcct gtcagttctg
    13261 acagtagctc cgcgccgccc ggattttcta tggtgcctgc ccaagaggca tcgtggagaa
    13321 cgcccaaaaa ccggcgcggc tgctgatcgt ggatgaccat ccgcccctgg cccgccaggt
    13381 ggagcagctg ctccggggtg aattcgacgt cgtgggcacc ctgaccagcg gggccgccct
    13441 ggacaccgcg gtcgccgaac tgcatcccga gctgatcgtc atggatatca ccctgcccgg
    13501 cgcgagcggc atcgagctgg cgcgccggct gacggcgagg ggctgcccgg cgcgcattgt
    13561 ctttctgacc gttcacgcgg acgccgacta tgcccgggaa agcatgaccg cgggcgggct
    13621 gggctacgtc atcaagccgc ggctcggttc ggacctggcg cccgcgctgc gcgcggcgcg
    13681 ggaggggcgg agcttcgttt ccccttgtcc cgaactggtc gggctgcagg gcccaggatc
    13741 cagcacatga acatgaagaa ggaatggaaa cacaggccac aaggagaaag agccatgagc
    13801 aatagagatg ttgccaagca atgcgcggcc ggcctcggcc gcgtgggaac ctgggccatg
    13861 gtcggctcgg tgctctccgc gggatcgctg gtatcgcccg catcggcgca gtcgacggaa
    13921 tccgccccgg ccgggaagga cgggggccat cccaacatcc tcgtcatctt cggcgacgat
    13981 atcgggcagg ccaacatcag cgtcttcacc aaggggctca tgggctacca gaccccgaac
    14041 atcgaccgga tcgcgaagga gggcatgctg ttcaccgact attacgcgga gcagagctgc
    14101 accgccggcc ggtcctcctt catcaccggc cagtgcgtct tccgtaccgg cttgagccgg
    14161 gtgggcgtgc cggcctcccc gatcgggatc cgcgccgagg acccgaccat cgccgagctg
    14221 atcaagcctc tcggctacgc caccggccag ttcgggaaga accatctcgg cgaccggaac
    14281 gagcacctgc cgacggtcca cgggttcgac gagttctacg gcaacctgta ccacctcaac
    14341 gccgaggagg agccggagaa ctacaactac ccgcaggatc cgaagttccg catgatgttc
    14401 ggcccgcgcg gcgtgctccg gtgcaaggcg acggacgtgg acgacccgac ggtggacccg
    14461 cgcttcggca agatcggcaa gcagaccgtc gaggacaccg gcccgctcac ccgcaagcgg
    14521 atggaaacga tagacgacga gacctcggag gcggccatcg atttcatgca gcgccaggtc
    14581 aaggccggcc agccgttctt ctgctggtac aacagcacgc ggatgcactt ccggacgcac
    14641 gtgcggcccg agcaccgcag cccgagaggc gcgaaggcgc ggacggagta cgccgatgga
    14701 atggtcgagc acgatggcac ggtcggctcg ctgctgaagg cgctggacga cctcggcatc
    14761 gcggacaaca cgatcgtcat ctacacgacc gacaacggcc cgcacgccaa cagttggccg
    14821 gacggcgcga cgactccgtt ccgcagcgag aagaacaccg gctgggaggg cgggttccgc
    14881 gtgccgtgct tcatccgctg gcccggccgc atcgaggcgg atcggatttc caacgagatc
    14941 gtcagcggcc tggactggct gcccaccctc ctggcggccg cgggcaaccc gaacatcaag
    15001 gaacagctgc tcgcgggcta ctcggcgggc gacagacagt tccgcgtcca cctcgacggc
    15061 tacaacctgt tgccgtacct caccggccag gcagcggaaa gcccgcgccg cgagttcttc
    15121 tacttcagtg acgacgggga cctgttgaac ctgcggtatg agaactggaa gatcgtcttt
    15181 gccgagcagc gctgcacggg cacgatggcc gtctgggccg agcccttcac gccactccgc
    15241 ctgcccaagt tcttcgacct gcgggccgat ccgtacgagc gggcggacgt gacctccaac
    15301 acgtactacg actggtacct cgaccacatg tacttcacga tcccggccgc gcagtacgcc
    15361 ttgaagttca tcgccacgtt caaggagttt ccgccgcgcc agaagccccc cgggtccgag
    15421 atcgaaaagg ccgtggatcg gctgctgcag gccgccgcag ggcgatagga gaggacagca
    15481 gatgaacaag cgggtttttc tcggtctcat gggaatcctg gccgccgcgg cgtgggccgc
    15541 ggcggccgac ccgctgccgt cgtggaacga cggcgcggcg aaggaagcca tccttcagtt
    15601 cgtggccgga acaaccacgg aggggtcgcc ggacttcgtg ccgccggccg agcgcatcgc
    15661 ggtgttcgac aacgacggga ccctctgggc cgagcagccg atgtacttcc aggggctctt
    15721 catgctcgac cgggtcaagc gcatggcgtc gcaacacccg gaatggaagg agcaggagcc
    15781 gttcgcctcg ctcctgaagg gcgacctgaa aacggccttc gccggcggcg aggctgcggt
    15841 cggccggctg ctgatgacaa cgcacgccgg catgaccagc gaggagttcg agaagatggc
    15901 cgccgactgg atcgccgggg cgcggcaccc gaagacgggc cggctgctca ccgagatggt
    15961 ctaccagccc atgctggagc tgctcgcctt cctgcgcgcg aacgggttca agaccttcat
    16021 tgtctccggc ggcggcatgg aattcatgcg gccgtgggcg gagcgggttt acggcatccc
    16081 gcccgagcag gtcatcggca gcagcatcaa gacgaagttc gagctgcgcg acggcgtccc
    16141 ggtgctcgtc cggatgccgg agagcgattt cgtggacgac gggccgggca agcccgtggg
    16201 catccagatg cacatcgggc ggcggccggt cgccgcgttc gggaactccg acggcgacct
    16261 ggcgatgctc cagtggaccg cggccgggcc aggcgcgagg ctgtgcctga tcgtccacca
    16321 caccgatgcg gaccgcgagt gggcatacga ccgcgaatcc cacgcggggc gcctggacaa
    16381 gggcctcgac gaggcggcgg cgcggggttg gacggtggtg gacatgaaga acgattggaa
    16441 acgcgtgttt gtcttcgagg cagagcaact ggtccggccc gatagcggac cccaataaga
    16501 ggtgagaccc atgatgcgag agaaccatgg aatccggcag gccggccggt gggcggcggt
    16561 aggctcggtg ctggccacgg gggcgctgtt cggcgcccgc gcgacgggac aggaagtgct
    16621 gccgacgccg ccgatgccgt tcaaggggca gatcgggttg agcgcggcgg attcgacgcc
    16681 cgacttcccg aaaccggtga aggcgccgcc gggcgccccg aacgtcgtgc tggtgctcct
    16741 ggacgacgtc gggttcggcg cgtcgagcac gttcggcggg ccgtgccaga cgcccacgtt
    16801 cgaccggctg gcgaagagcg gcctgcgttt cacgcagttc cataccacgg ccttgagctc
    16861 gcccacgcgc gccgcgctgc tgaccgggcg caaccatcac tcggtgcaca cgggcgtgat
    16921 catggagcag ggcacgggct acccgggtta tgactccctg atgggcgggg acaccgcgac
    16981 gatcgccgag atcctgaagc agaagggctg gaacacctcc tggttcggaa agaaccacaa
    17041 cgtgcccgac tggcagagca gccaggccgg cccgttcgac ctgtggccca tcggcctcgg
    17101 gttcgaacac ttctacggtt tcgtctgcgc cgagaccagc cagtggcggc ccgcggtcac
    17161 cgaggggacc acgcccatcg agccgtacct cggcaacccg gactaccact tcgactacga
    17221 catcgccgac cgggccatcg agtgggtccg caaccagaag gccgtggcgc cggatcgccc
    17281 gttcttcctc tattacgcgc cgggggcgac ccactcgccg caccacgcga agccggaatg
    17341 gatcgccaaa tacaaggggc gcttcgaccg cggctgggac gcggtgcgcg aggagacgct
    17401 cgcaaagcag aaggaactgg gcctggtccc ggccgacacg cagctgaccc cgcgcccgga
    17461 gcgcatcccg gcctgggaca cgctcaacgc ccagcagaag gacctctacg cctacatgat
    17521 ggaggtctac gccgggttcc tcgagcagac ggactacaac gtcggccgcg tgctcgaagg
    17581 gatcgggaag atgggccaac tcgacaacac gctggtcatc tacatctgcg gcgacaacgg
    17641 ggccagcgcg gagggcatgg ataatggcga gctcaacgaa ctcgcgggcc tgaacggcgt
    17701 cgacgaggac tacgccgagg tgctgaagca caaggacgac ctgggcacct ggaagacgca
    17761 caaccactac ccggccggct gggcgcacgc catgtgctcg ccgttccagt gggccaagca
    17821 gatcgcctcg cactacggcg gaacccgcaa cgggatggtc atctcctggc cggcccggat
    17881 caaggacgcg ggggggatcc ggccgcagtg gcaccacgtg atcgacgtcg tgccgacggt
    17941 cctcgaggcc tgcggcctgc aggccccgtc catcgtcaac ggcgtggcgc agaagccgat
    18001 cgagggcgtg agcatggcct acaccttcga caacgcgcag gcgccgacgg cgcgccggac
    18061 gcagtacttc gagatgttcg gcaaccgcgc gatctatcac gatggctggg tcgcgtgcac
    18121 gaccccgcgc gcggcgccgt ggcaggatac ctcccggctg cctccggtgg acatcatcag
    18181 cggatatcgg tgggaactat atcatgtcgc cgaggacttc agcgaggcgg tcaacctggc
    18241 cgaccagaac ccggacaagc tgcgcgagct gcaattgctg ttttacgccg aggcggcgaa
    18301 gtacaacgtc ctgccgctgg acgacagcaa gatcgagcgc ctgaacccgg ccatccggcc
    18361 cagcctgacg cgcggccgga cggaattcac gtactacggc ccggtgcggc gcattcccga
    18421 aggagccgcg cccgacgtga aaaacaagtc gtggagcgtc gccgcggacg tggtcctcgc
    18481 gaccggcgcg gagcagggcg tgctcgtgac gcagggcggc ctgttcggcg gttacgccct
    18541 gatgctgcgc gacggcaagc cggtgttcca ttacaacatg ctgaatgtgg cccacttcaa
    18601 catcgccgcg acgacggcgc tcgctcccgg caagcacacc atcgcgttcg agtaccagca
    18661 cgacagcggg gtggggctgg gccggggcgg caacgggatc ttgagcgtgg acggccagcc
    18721 ggtggcccag ggccgcatcg agcgcacgat cccgctccgg ttcaccctgg acgagacctt
    18781 cgacatcggc gagg
//
LOCUS       DAOOTK010000054        18354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2091_length_18354_cov_4.823925, whole genome shotgun sequence.
ACCESSION   DAOOTK010000054 DAOOTK010000000
VERSION     DAOOTK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18354
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_18354_cov_4.823925"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK634_07745"
     CDS             <1..469
                     /locus_tag="PK634_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97665.1"
                     /translation="GGTTASVDESLEEALFWARDHIVDHVMLPDVHPQLHDAVLGRRF
                     KAEGGSMAIIETPTVSCNVRAAELALKGTPVSLTEIRLADSWLAGKGVSLYRGELHDI
                     EAAVDLAVSFLQQTGVRPVHRIIPAPHEALGLQADAGPYFPDTAPTELEGENF"
     gene            471..761
                     /locus_tag="PK634_07750"
     CDS             471..761
                     /locus_tag="PK634_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOW97666.1"
                     /translation="MLLGKVMGRVVLCEAYKGLETVPMLWIQPLDKKGQPKGKPVVAA
                     DSTRMAGPGEFVYYEGGREAAMTLDPWFVPVDHAVVGIVDNLHLADDKELAP"
     gene            758..1030
                     /locus_tag="PK634_07755"
     CDS             758..1030
                     /locus_tag="PK634_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOW97667.1"
                     /translation="MIIGRVIGEIHATIQHPFYAGKKLMVVEKISPEGKPLEDYVVAV
                     DSVGAGPGERVLIVDEGNSARQIVHSTTAPLRSIIVGIIDEITTAN"
     gene            1131..2309
                     /locus_tag="PK634_07760"
     CDS             1131..2309
                     /locus_tag="PK634_07760"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOW97668.1"
                     /translation="MKIILIVLDSIGIGEMPDAAKYGDVGSATLPHLAKAAGGLKAPV
                     MQSLGLGNIQALLPGGKPIDGVPPAGKPLASWGAMQEVSDGKDTVTGHWEIAGLVTIP
                     GFSVFPPNYPSFPPELVEAFEKRTGRKILGNKAASGTAIIDELGPAHMKTGKWIAYTS
                     ADSVFQIAAHEEIVKIPELYKACEIARELCDKYKVGRVIARPFVGKPGAYMRTENRRD
                     FAFKPEESTVMERLVAAGVPVYAVGKIEDIFAHRGISESNHTGNNQASQQAVTAWTKE
                     KKKGLIFANFIDFDMTYGHRRDAKGYARSIEQTDQWLASYLPLLGKDDVLMITADHGN
                     DPTYKGTDHTREFVPLLVYRPGALALNLGIRQGFFDIAQSIAAYFGLPPLPRGTSFLS
                     "
     gene            2494..3255
                     /locus_tag="PK634_07765"
     CDS             2494..3255
                     /locus_tag="PK634_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOW97669.1"
                     /translation="MTGRMGLTAAAVLCVFAVAASAEWERTIFAWDTRLPAQGKFQAS
                     LWGDYYAYEVGDADATTLDGVLYLTYGIADNWSACVAPSFTSWDVDDGDSESGISDTL
                     LTTTYRFMEEKDSGADVAILGRASLPTGDDDKGLGSGNVEPGFLVLAAKTLGPIVAVA
                     NAGATAILDADDGEEDFILRGCIEGIYPLNEQLNINALLSAETARADGADDDVELGLG
                     TRFTPVEQFFVLGAGYVCLTDAYDWGATLATGYEF"
     gene            3377..4684
                     /locus_tag="PK634_07770"
     CDS             3377..4684
                     /locus_tag="PK634_07770"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOW97670.1"
                     /translation="MLPQWIIEKKRDGQALSEEEIRCFIEGYTVGRIPDYQMAAMAMA
                     IYCRGMTAAETAVITDAMMRSGDLVDTSSIKRPKVDKHSTGGIGDKVSLILAPLVACC
                     GVAVPMISGRGLGITGGTLDKMESIPGYRTDLSVKEFVRVIQRCGCSIIGQTGRLAPA
                     DKKLYALRDVTGTVPSIPLITASIMSKKLAEGIDALVLDVKWGKGAFMRTIEDARALA
                     RSMVNVGTHMGKGMAALITDMNRPLGRCAGNALEVIESVQTLKNEGPADLVEVTLALS
                     ARMLLLAKKAKNEKDARAQLKKHLASGKAYEVFQEMVRLQGGDAGSLEKLHQIHAASL
                     QEPMPATAAGTIAAVDAERVGKACVILGAGRTKTDDKVDFAVGVSGLMQVGEKVTKGQ
                     PLVVIHANDENRLEEARKLLDGAFTIRKGAVKEPPLVYETILK"
     gene            4777..5607
                     /locus_tag="PK634_07775"
     CDS             4777..5607
                     /locus_tag="PK634_07775"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOW97671.1"
                     /translation="MDQKRLDKALAFVKSQWPAAKPKVGLICGSGWSDVVAAFKEKAS
                     LSYGNIPGMGSPGVVGHAGKLAWAGLSGIETFVFQGRRHWYEGEGWTPVALPVYLLKQ
                     LGAKAVVLTNAAGGVRADLTPGTLMIIEDHVNLMNANPLLGPHNPAWGPRFPDQSTVY
                     NKELREALERAGKAVGQKLAKGVYLAGSGPLYETPAEIRAWRALGADAVGMSTVPEAQ
                     LANAAGMKVLGLSCITNLAAGVSKNPLTHEEVTATTKAAMAGMKTLIVQLWKELATAV
                     "
     gene            5621..6385
                     /gene="udp"
                     /locus_tag="PK634_07780"
     CDS             5621..6385
                     /gene="udp"
                     /locus_tag="PK634_07780"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOW97672.1"
                     /translation="MSDINKDVQYHINMKPGDIGRYVLLPGDPGRVPKIASFFDDAKE
                     VTFNREYRTYTGTVAGIKVSCTSTGIGCPSTAIAIEELVKIGADTFIRIGTAGALQPE
                     IKLGDLCITTASVREEGTTRQYVPLSYPAVANLDVTVALREAAKKLGFRHHCGIGHCK
                     DAFFIEGDEGLPLAAENKNKWDAWYKSNVLSTSMEAAALFVVSSIRRVRAGEVLAIIG
                     LTYEDKPIVAKVGIEDAIKTAIEAVQILARQDGVAK"
     gene            6382..6825
                     /locus_tag="PK634_07785"
     CDS             6382..6825
                     /locus_tag="PK634_07785"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOW97673.1"
                     /translation="MKPCSLAQLPEPERRLVLEAVAARRKAYAPYSHYKVGAALLDEK
                     GAMHSSCNVEGADYTLTTHAEMGAINAMVRSGVLRLKMMAVAVQSGIGWGMPCGLCRQ
                     KIREFAAGTDVPVLGIKLDEKEEIFEIWASSLEELLPYSFGPEHL"
     gene            complement(6848..8290)
                     /locus_tag="PK634_07790"
     CDS             complement(6848..8290)
                     /locus_tag="PK634_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOW97674.1"
                     /translation="MNHRTRQLVRRIISHVWGPTGSILLHLLVVFLLFKLVVGEPPRK
                     IEEFTITYLPVVPPPPDAFDPPDNKPWDDLPPTPVDMPVQPDVPSSEPPPDFKADDWM
                     ARGPQELAGPPILDDVRSSAILPVGYIQRTTAGRMNGIASYAGGGYSEPSVVKALEWL
                     KKNQAPDGSWGPNKPAMTGLGLLTFLAHGETLSSDAYGETVGRAVRFLMTQQNEAGQF
                     HSTDGQAAPYVHAIATYAVSEAYGMMRVPQLRPVMEKAVGVIVTGQQPGGGWDYRYAK
                     AGRRDTSVAGWQIQALKAARIAGAETPGLAEALERASADLLAVRDPETGRFYYTDRTS
                     HKTDSITAIASLCLQLLGRGRSGAVDSAFRAMGQAACDWEKPGEWPMYAWYYLTQTHF
                     HEGGMAWSRWNGQFSKTLVRHQNEDGSWTSPAGVRGGAGSEAHLGPVYSTTLAALSLQ
                     VYYRVLPTYKQVAAEPVTQAAKNDIGVEIL"
     gene            complement(8406..9053)
                     /locus_tag="PK634_07795"
     CDS             complement(8406..9053)
                     /locus_tag="PK634_07795"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOW97675.1"
                     /translation="MKKRPGAAILFSGGADSMAVAIHYLRRGRPVALLTCYNGAESMM
                     EHAQHRAGLLQKRFRGLVAWEQRDVKDLFHRLAIQHLESDIKTYGTLVCCGCKLAMLA
                     EAILLCRERGLRELADGFRREQIYYPEQTDEYMTPAGRLARSFEIRYAHPLYEATDAD
                     VPRMAAEEGLPPVALQPSCLFAFNRIRPSAKIRQYVNAKLPAVRAFIKKRLATVK"
     gene            complement(9156..10370)
                     /locus_tag="PK634_07800"
     CDS             complement(9156..10370)
                     /locus_tag="PK634_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOW97676.1"
                     /translation="MAYDLIIRKAQLRGKKGLVDIAIEKGVIAKIAAKIAGRAGKVID
                     AGGRLVTESYVNTHLHLCKVYTLALQDTKALSAYHGESMGAAMTAIELAARVKDKYNE
                     KWIVPNVLKALAHAARNGNTHIRAFADVDSKAKLIGVKSLIKARDMFKGIVDVQVVAF
                     PQDGVVREPGTADLIRQAMDLGADVVGGIPWIEYTNADSQKHVDEMAKIAQAYDADLS
                     MLVDDAGDPGLRTLEMLALKTIETGRIGRSLAHHARAMCMYPEPYFRKVAALLRKAGM
                     GVVSDPHTGPLHARVKELLAEGNLVCLGQDDIADAYYPFGQNSMLEVAFLNVHLLWMT
                     TFPEMEKIYDMITTAPAKAINVKNFAVKEGAPAHLVVLDAPTVYEALWYHREPLHVIS
                     HGKLVDLKNLPA"
     gene            complement(10400..10885)
                     /locus_tag="PK634_07805"
     CDS             complement(10400..10885)
                     /locus_tag="PK634_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOW97677.1"
                     /translation="MAEKINLTVNGVPRTVEIEGWERLIDVLRDKLDLTGAKRGCDDA
                     TCGACTVVLNGEAVQSCITGLAKYKDGNVVTIEGLSGGVGKLHPIQQALVDAGAVQCG
                     YCTPGIVLRLYALFGKKPDATDDEIVDALEIHLCRCTGYEAIFEGAKLARGYLKKAAK
                     A"
     gene            complement(10974..13292)
                     /locus_tag="PK634_07810"
     CDS             complement(10974..13292)
                     /locus_tag="PK634_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOW97678.1"
                     /translation="MEFKYIGKDILRPDATAKITGKAKFLADLKLPGMLHCAILRPTV
                     AHAKIVSIDTSEAEKCPGVVKVVTAQGQNYRYGDNIRDFTPMAGDRVRHIGEAVAAVI
                     ADSAHHAREAVKKIKPVYEPLPVYVDARDALKPDAVLIHPDSGDFWHLPGLGPKAGTN
                     IANYYKLVKGDVKKGFADAEVTVEGEFNYPFGSCSAIEPHGAIAWFHEDGLIEIWSSS
                     ICPFVIRDEVARVYDRPISDVRVHIPEVGGCFGYKSDITVEQTIAFIASFVPGRPVKW
                     VATRKEDLVSTLMGHGIRTKMKLGAKKDGTLTAIQATLYHSTGAYADTGIHVCIGATH
                     NCTGPYEFPNCFMESYLTYTNTPPIGAFRGYGHQEGQFGVERLMDMLARKLKMSPFAL
                     REKNYLGEGKTTSLGEKLWKTNGDVRACVANVKKAVFDSPKPKEDEQFYYGRGFVAVM
                     KSPKGAPFSSKGCYLKMNSDGSVCINMGGAEVGQGLRNVVRLVAAEALKIPPERIRVY
                     TEIDTQFSPWEWQTIGSMFTTQGGRAIVRAAEKAIAILKRTAGQVLKCDEDMLDYDGE
                     FVFLKSDPGVRVEVPRLIHGYITGDGITIGEVVQTTSDCRLPRYSGLDPETGQGSAGV
                     EYTFGAQACDIRIEKATGKVLIDHFASSFDVGRVIQPKQIRGQIVGGVMIAIGSTLHE
                     ELKFDKDGKCLNPFFGRYRFPTIKDRPAKQTVVCVETPAVVGPFGARGIGEHPLVGVA
                     PCILNAIQDAIGVEFTELPVTPDKVKKALEGR"
     gene            13462..>18354
                     /locus_tag="PK634_07815"
     CDS             13462..>18354
                     /locus_tag="PK634_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOW97679.1"
                     /translation="MTRTTCLFTLAVCAALGLSLPGRAVAGDPNPATPEDESANWTVT
                     DPVDQDFSEGTPAPASREAAPAVLPVSIHQAEAEYYKQFRAVTESDWDAVRRDLREKE
                     LGAAPRALTGASTPGPFTLQKEVFGWYPSWEGSSYTNFEFTLLSTVAYFNYEVNAGTG
                     YAVTMNSWSNTPLVYWAHSNNVDVVLCATLMSNHAAFFGSAAAQSNLINELVRVVQLR
                     GAEGVNIDFEAIASGYAEELTLFMSNLTVRMHAQVPGSQVSICLPSVDWSSNFDVAEY
                     EGFLDKCIIMGYDYSWSTDDQAGPVAPLKGSAIFSSWCVQKSVTNYLDLGLSPGKLLL
                     GCPYYGYDWPTVSHAMHADTTGDGTARQYPSAESYAGTYGYNWDTNSLTPYVLYGSYR
                     QLWYDDTNSLGLKYDFVNMKELAGIGIWALNYDEGTSDLWDLLASKFAGEADWQSQDP
                     GTTASFYGVDALNGQWAAVGAGGVISTSGDDGLNWTSRVSGTGSLLLNVVGGGGLWVA
                     VGDTGTVVTSSGGATWTTRSTPTNAMLRGAAYGNGAYVTAGAGGVILRSTDGSTWAQQ
                     VSGTTNTIQGVNYVGSLFIATGENSVLLTSSDGISWTSRTSNAGGWLLDAAYGNGAYV
                     AVGIGSRVVTSPDGTTWTRQTNDLPSASANLYRVAFGSGQFVAVGQGGVIWGSTDGSN
                     WTAEVSGTTNFLRGVAFSNDLFMAAGYNGTILTKGAGAPSVEIATAGATVPNATSSQT
                     VTGAANAYVVGTMRWTNSLGGSGTFAAATPWSFSASLSVGTNVLTVTGTNTAGSAASD
                     SVSFIRQAAEGGGGSGSETTAAAQPGGALSDVVVFTSAGHGFTYNSTYGWITGRGLDN
                     QVVEDIGNIDQLNYFAEYCFKAGATVVPFRPLGQQTNEVVLDNTNSARVTFGGTWSNS
                     TSSTYYGEAGATPYRYAYINSTATTAWAIYRPNLPAAGFYPVYAWARSGSDRVKQLYR
                     VYHSGGVTDVRVNHRRVGLGWVWLGTYYFDAGTNHSVNISNYAPDGYLATDVVIADAI
                     RFGNGMGNIDRGAGVSGLERELEASRYWVQSMVGQGMSSTLYDSAGLDDTDDNVGAPN
                     RMAYEMNRESDGGYFDRIYLGFHSNAGSGAGRGPMGLYDTRFSAPYSGMQQVYATKVA
                     AELTNDLGYGDLGVWFPDSFTNNTANLYGSMYGEIFGAIVSNEMNSTIIEVGYHDNLH
                     DTYLLRCPSARRVMAMASYQAIVKYLSASNAAVSSVLLPDPPTDVSAVNDGAGAVTVA
                     WQAPVTNKAGGSAATGYIVYRSTNGFGFGNPVTVPGAATLSATLTNMTAGQIYYFQVC
                     ATNAGGESLASKTVGVRVSPAGYATHLVVNGFERNERRLTPTRYYAYNIDGDVARVIQ
                     RTINSQDYVIQYGTALAAAGRYFDSCDNDAVADGDIDLTDYFAAYWILGRESTADETI
                     SDAEQALVTAFLNQSNRLFVSGTELGWDLQQYGAASDKSFCSNYLKTAYVADDAGTNV
                     AAARASGVFGGLANLVFDYTGTGPVYRAAYPDTLATAGSPASTAALVYGTSAGGASVA
                     AISYSNQYRLVVMGFPFETILDASARTNLMRRTLDFFGSAPADSPVTDITNGNASVAN
                     AVGSIVIGGTNNPAVIGQMTW"
BASE COUNT         3085 a         6249 c         5991 g         3029 t
ORIGIN      
        1 cggcggcacg acggcctccg tggacgagtc gctggaggag gcgctgttct gggcgcggga
       61 ccacatcgtc gaccacgtca tgctcccgga cgtgcacccg cagttgcacg acgccgtgct
      121 cggccgccgg ttcaaggccg agggcggctc gatggccatc atcgagacgc cgaccgtctc
      181 ctgcaacgtc cgggccgccg agctggcgct caagggcacg ccggtgagcc tgacggagat
      241 ccgcctcgcg gacagctggc tggccggcaa gggcgtgagc ctctaccgcg gcgaactgca
      301 cgacatcgag gccgcggtgg acctggcggt ttctttcctg cagcagacgg gggtgcggcc
      361 cgtgcaccgg atcatcccgg cgccgcacga ggcgctcggc ctgcaggcgg acgccggccc
      421 ctatttcccg gatacggcgc cgacggaact cgaaggggag aacttctgac atgctgctcg
      481 gcaaggtcat gggtcgcgtg gtgctgtgcg aggcgtacaa gggcctcgag accgtgccca
      541 tgctctggat ccagccgcta gacaagaagg gccagcccaa gggcaaaccc gtcgtcgcgg
      601 cggattccac gcgcatggcc ggacccggcg aattcgtcta ctacgagggc ggccgcgaag
      661 ccgccatgac cctggaccca tggttcgtgc ccgtcgacca cgccgtcgtg ggcatcgtgg
      721 acaacctgca cctggccgac gacaaggaac tggcgccgtg atcatcggcc gcgtcatcgg
      781 cgagatccac gcgaccatcc agcacccgtt ctatgccggg aagaagttaa tggtcgtcga
      841 gaagatctcc cccgagggca agcccctcga agattacgtc gtcgcggtcg actcggtcgg
      901 cgccgggccc ggcgagcgag tgctgatcgt cgacgaaggc aacagcgccc ggcagatcgt
      961 ccattccacg accgcccccc tccgctccat catcgtcggc atcattgacg agatcacaac
     1021 cgcgaattga cgcgcattat cgctaattcg gccggatttc ccattcgcgt taattggcgt
     1081 caattcgcgg tcctctttgc gctcctcgtg ccttctgtta tattgtcgtt atgaaaatta
     1141 tcctgatcgt cctcgattcc atcggcatcg gcgaaatgcc ggacgcggcg aagtacggcg
     1201 acgtcggctc ggccaccctg ccccacctcg cgaaggcggc cggcggactg aaagccccgg
     1261 ttatgcagtc cctcggcctg gggaatatcc aggcgctgct gcccgggggc aagccgatcg
     1321 acggcgtgcc gcccgcgggg aaaccgctgg cgtcgtgggg cgcgatgcag gaggtctccg
     1381 acggcaagga cacggtcacg ggccactggg aaatcgcggg gctggtcacc atcccggggt
     1441 tcagtgtctt tccgccaaac tacccgtcgt tcccgcccga actggtcgag gcgttcgaaa
     1501 agcgcaccgg ccggaagatc ctcggcaaca aggcggccag cggcacggcg atcatcgacg
     1561 agctgggccc ggcgcacatg aagaccggga agtggatcgc ctacaccagc gcggactcgg
     1621 tcttccagat cgccgcgcac gaggagatcg tcaagatccc ggagctctac aaggcctgcg
     1681 agatcgcgcg cgagttgtgt gacaagtaca aggtcggccg ggtcatcgcc cgcccgttcg
     1741 tcggcaagcc cggcgcgtac atgcgcaccg aaaaccgccg tgactttgcg ttcaagccgg
     1801 aagaatcgac cgtgatggag cggctcgtgg cagccggcgt gccggtctat gccgtcggca
     1861 agatcgagga catcttcgcg caccgcggca tctcggagtc caaccatacc ggcaacaacc
     1921 aggcctcgca gcaggccgtg acggcgtgga cgaaggagaa aaagaagggc ctgatcttcg
     1981 ccaacttcat cgacttcgac atgacctacg gccaccggcg cgacgcgaag ggctacgccc
     2041 gctcgatcga gcagaccgac cagtggctgg cctcgtacct gccgctgctc gggaaggatg
     2101 atgtgctgat gatcaccgcg gaccacggca acgacccgac gtacaagggc accgaccaca
     2161 cccgcgagtt cgtgccgttg ctggtctacc ggcccggcgc gctcgccctc aacctgggga
     2221 tccggcaggg cttcttcgac atcgcgcaat ccatcgcggc ctacttcggc ctgcctcccc
     2281 tgccgcgggg gacgagcttt ctttcttgat gggcgccggc ggcacagcgc gaggtcgatg
     2341 ccttcggccc cgccgcccgt gtatcacagt ttacaaactg tgatacatgc ctcctcgacc
     2401 ccaaggcgga gtttccttgt tgccctcggt acagccgtcg atcataatgc agtcaaccgg
     2461 ggcgtaactc ccggcacgaa aggggaacac gacatgacgg gtagaatggg actgactgca
     2521 gcggctgttc tgtgtgtctt cgctgtcgcg gcgagcgccg agtgggagcg gaccatcttc
     2581 gcgtgggata cgcgcctccc ggcccagggc aagttccagg cgtccctgtg gggcgactac
     2641 tacgcatacg aagtcggcga tgcggacgcc accacgctgg acggggtgct gtacctgacc
     2701 tacggcatcg cggacaactg gtcggcatgt gtggcaccct cgttcacaag ttgggacgtg
     2761 gacgacggcg actccgagag cgggatttcc gacacgcttc tcacgacgac ctaccgcttc
     2821 atggaggaaa aggactccgg ggcggacgtg gccatcctgg gccgcgcgag cctgccgacg
     2881 ggcgatgacg acaagggcct cggcagcggc aacgtggagc cgggcttcct ggtcctggcc
     2941 gccaagacgc tcggaccgat cgtggccgtc gccaacgccg gcgccaccgc gatcctcgac
     3001 gcggacgacg gcgaggagga tttcatcctc cgcggctgca tcgagggcat ctacccgctg
     3061 aacgagcagt tgaacatcaa tgccctcctg tccgccgaga ccgcccgggc ggacggcgcg
     3121 gacgacgatg tggaactcgg cctcggcact cgcttcacgc ccgtggagca gttcttcgtg
     3181 ctgggcgccg gctacgtctg cctcaccgac gcgtacgact ggggcgcgac gctggccaca
     3241 ggctacgagt tctaaccgcc gcgcgaccgg ctgatgatga aagagccgcg ggctccgcgc
     3301 ccgcggctct ttgcgtcctt actcgcccgt gctcattcct tgactttacc ttcccggatc
     3361 aacaggatgc cccattatgt taccccaatg gattattgag aagaaacgcg atggccaggc
     3421 gctttccgag gaggaaatcc gctgtttcat cgagggctac accgtcggcc gcatcccgga
     3481 ttaccagatg gccgccatgg ccatggccat ctactgccgg ggcatgaccg ccgccgagac
     3541 cgcggtgatc accgatgcca tgatgcgctc cggcgatctc gtggacacct cctccatcaa
     3601 gcgccccaag gtggacaagc actccaccgg cgggatcggc gacaaggtct cgctcatcct
     3661 cgcgccgctc gtggcatgct gcggcgtcgc ggtgcccatg attagcgggc gcggcctcgg
     3721 catcacgggc gggacgctgg acaagatgga atccatcccc ggctaccgca cggacttgag
     3781 cgtgaaggag ttcgtcaggg tcatccagcg gtgcggctgc tcgatcatcg ggcagaccgg
     3841 ccggctggcc cccgcggaca agaaactata cgccctgcgc gacgtgaccg gcaccgtgcc
     3901 gtccatcccc ctgatcaccg ccagcatcat gtccaagaag ctcgccgagg gcatcgacgc
     3961 gctggtgctg gacgtgaagt ggggcaaggg cgcgttcatg cgcacgatcg aggacgcccg
     4021 cgccctggcg cggagcatgg tgaatgtcgg cacgcacatg ggcaagggca tggcggcgct
     4081 gatcacggac atgaaccgcc cgctcggccg ttgcgccggc aacgcgctgg aggttatcga
     4141 gtccgtccag accctcaaga acgaaggccc ggcggacctg gttgaagtga ccctcgccct
     4201 ttccgcgcgc atgctcctgc tcgcaaagaa ggcaaagaat gaaaaagatg cgcgggccca
     4261 attgaagaag cacctggcct ccggcaaggc ctacgaggtg ttccaggaga tggtgcgctt
     4321 gcagggcggc gatgccggct cgcttgaaaa gctgcaccag attcatgccg ccagcctgca
     4381 ggagccgatg ccggcgacgg cggcgggaac catcgcggcc gtggacgcgg aacgcgtcgg
     4441 caaggcctgc gtcatcctcg gcgcgggccg gacgaagacc gacgacaagg tagacttcgc
     4501 cgtgggcgtc tccggcctga tgcaggtcgg cgagaaggtg accaagggcc agccgctcgt
     4561 ggtcatccac gccaacgacg agaaccggct ggaggaggcg cgcaagctgc tcgacggcgc
     4621 gttcacgatc cgcaagggcg cggtcaaaga gccgccgctg gtgtacgaaa caatcctgaa
     4681 ataacgaaaa ggaacagaag gcgccaagct cgcaaaggtg atggacacga acttggcgat
     4741 ctttgcgcct cctgttcaat gatcggggga tccgttatgg accaaaagag gctggataaa
     4801 gccctggcat tcgtgaagtc gcaatggccc gcggcgaagc cgaaggttgg actgatctgc
     4861 ggctccggct ggagcgacgt ggtggcggcg ttcaaggaga aggcctccct gtcgtacgga
     4921 aacatccccg gcatgggctc gccgggcgtc gtcggacacg cgggcaagct ggcgtgggcc
     4981 gggctctcgg gcatcgagac cttcgtgttc cagggccggc ggcattggta cgagggcgag
     5041 ggctggacgc ccgtcgcgct accggtttac ctgctgaagc agctcggcgc gaaagccgtg
     5101 gtcctgacca acgccgcggg cggggtccgg gccgacctga cgcccggcac gctgatgatc
     5161 atcgaggacc acgtcaacct gatgaacgcc aaccccctgc tcggcccgca caacccggct
     5221 tggggcccgc gcttcccgga ccagtccacg gtgtacaaca aggaactccg cgaggcgctg
     5281 gagcgcgcgg ggaaggccgt cggccagaag ctcgccaagg gcgtctacct cgccggttcc
     5341 ggcccgctct acgagacccc ggcggagatc cgggcctggc gcgcgctggg cgcggacgcg
     5401 gtcggcatgt ccaccgtgcc ggaggcccaa ctcgccaacg cggccggcat gaaggtgctc
     5461 ggcctttcgt gcatcacgaa cctcgccgcc ggcgtcagca agaacccgct gacccacgag
     5521 gaggttacgg ccaccacgaa ggccgccatg gcggggatga agacgctgat cgtgcaactg
     5581 tggaaggaac tcgcaacggc tgtttagccg ggagaaagcc atgagcgaca tcaacaagga
     5641 cgtccagtac cacatcaaca tgaagcccgg cgacatcggg cgctacgtcc tgctgcccgg
     5701 cgacccgggc cgcgtgccga agatcgcctc gttcttcgac gacgcgaagg aagtcacctt
     5761 caaccgcgag taccgcacgt acaccggcac ggtcgccggg atcaaggtct cgtgcacgtc
     5821 cacgggcatc ggctgcccct cgaccgcgat cgccatcgag gagctggtca agatcggcgc
     5881 ggacacgttc atccgcatcg gcaccgccgg cgccctgcag ccggagatca agctgggcga
     5941 cctgtgcatc accacggcct ccgtgcgcga ggaaggcacg acgcgccagt atgtgccgtt
     6001 gagctatccc gccgtggcga acctcgacgt caccgtcgcc ctgcgcgagg cggcgaagaa
     6061 gctcgggttc cgccaccact gcggcatcgg gcactgcaag gacgcgttct tcatcgaggg
     6121 cgacgagggc ctgccgctcg cggccgagaa caagaacaag tgggacgcct ggtacaagtc
     6181 caacgtgctg tccacctcga tggaggccgc cgcgctcttc gtcgtgtcct ccatccgccg
     6241 cgtgcgcgcg ggcgaggtcc tggcgatcat cgggctgacc tacgaagaca agcccatcgt
     6301 cgccaaggtc ggcatcgagg acgcgatcaa gacggcgatc gaggccgtgc agatcctggc
     6361 ccggcaggac ggggtcgcga agtgaagccg tgctcgctgg cgcagcttcc cgagccggag
     6421 cggcggctgg tcctcgaggc cgtcgccgcg cgccggaagg cgtacgcccc ctactcccat
     6481 tacaaggtcg gcgcggcgct gctggacgag aagggcgcga tgcattccag ctgcaacgtc
     6541 gagggcgcgg actacacgct gaccacgcac gcggagatgg gcgcgatcaa cgcgatggtg
     6601 cgctccgggg tgctccgcct gaagatgatg gccgtcgcgg tccagagcgg gatcggctgg
     6661 ggcatgccgt gcgggctgtg ccgccagaag atccgcgagt tcgccgccgg gacggacgtc
     6721 ccggtgctgg ggatcaagct ggacgagaaa gaggagatct tcgagatctg ggcctcctcc
     6781 ttggaggaac tgctgccgta ctcgttcggg ccggagcacc tgtaatcgcc ggccaggcca
     6841 catcgggcta cagaatttcc acgccgatat cgttcttcgc cgcctgcgtc accggctccg
     6901 ccgcgacctg cttgtaggtc ggcagcaccc ggtaataaac ctggagcgac agcgccgcga
     6961 gggtcgtgct gtaaaccggg cccaggtgcg cctcgctgcc cgcgccgccg cgcacgccgg
     7021 ccggcgaggt ccaactcccg tcctcgttct ggtgccgcac cagagtcttc gagaactgtc
     7081 cgttccaccg cgaccaggcc atcccgccct cgtggaaatg ggtttgcgtc aggtagtacc
     7141 aggcgtacat cggccactcg cccggtttct cccagtcaca tgccgcctgc cccatcgcgc
     7201 ggaaagcgga gtccacggcc ccggatcggc cgcggcccag tagttgcagg cacaacgacg
     7261 cgatcgcggt gatcgagtcg gtcttgtgcg atgtacggtc cgtgtaatag aaccgtccgg
     7321 tctcgggatc gcgaacagcc agcaggtctg cggaggcgcg ttcgagagcc tccgccaggc
     7381 ccggtgtttc cgcccccgca atccgggcgg ctttcagcgc ctgaatctgc cagccggcga
     7441 ccgacgtgtc gcgccgtccg gctttcgcgt agcggtagtc ccaaccgccg cctggctgct
     7501 ggcccgtgac gatcaccccg acggcctttt ccatcaccgg gcgcagttgg gggacgcgca
     7561 tcatgccgta ggcctcgctg accgcgtagg tcgcgatggc atgcacgtag ggcgcggcct
     7621 gtccgtcggt gctgtggaat tgcccggcct cgttctgctg ggtcatcagg aagcgcaccg
     7681 cccttccgac cgtctccccg taggcgtccg aggatagcgt ctcaccgtgg gcgaggaacg
     7741 tcagcaggcc caggccggtc atcgcgggct tgttcggccc ccaggagccg tcgggggcct
     7801 ggttcttttt cagccactcc agcgccttca ccacggaggg ttccgaataa ccgccgccgg
     7861 cgtagctggc gatgccgttc atccggcccg cggtcgtacg ctgaatgtat cccacaggaa
     7921 ggatcgcgga actccgcacg tcatccagta tcggcggacc cgccagttcc tgtggcccgc
     7981 gggccatcca atcgtcggcc ttgaaatccg gcggcggctc ggaagacggc acatccggct
     8041 gcaccggcat gtccacgggc gtcggcggca ggtcatccca aggcttatta tcgggtgggt
     8101 cgaaagcgtc cgggggcggc gggacgaccg gcaggtaagt gatggtaaat tcctcgatct
     8161 tgcgtggcgg ctcgcccacg accagcttga acagcaggaa aacgaccagc aagtgtaaca
     8221 ggatggaccc cgtcgggccc cagacatggc tgatgattct gcgaacgagt tgccgggttc
     8281 gatggttcat ggcatcctcc gctgctcctt gatacgagcg gcggacgcgg tcggatgaca
     8341 aggattggag ccgggacgcc cccgtcccgc aacgcgccgc gagggcgcgg cggctccatc
     8401 cgacactact tcacggtcgc gagccgcttc ttgatgaacg ccctgaccgc gggcagcttg
     8461 gcgttgacgt attgccggat tttcgcggac gggcggatcc ggttgaaggc gaacaggcac
     8521 gagggctgga gcgcgaccgg gggcaacccc tcctccgccg ccatccgggg cacgtccgcg
     8581 tccgtcgcct cgtacagcgg gtgggcgtac cggatttcga acgagcgggc caggcgcccg
     8641 gcgggcgtca tgtattcgtc cgtctgttcc gggtagtaga tctgttcgcg ccggaacccg
     8701 tccgccaatt cacggagccc gcgttcgcgg cacagcagga tggcctccgc cagcatcgcc
     8761 agcttgcagc cgcagcagac cagcgtgccg tatgtcttga tgtcggattc cagatgctgg
     8821 atggccagcc ggtggaacag gtccttgacg tcgcgctgct cccacgcgac caggccacgg
     8881 aacctcttct ggagcaggcc cgcgcggtgt tgcgcgtgct ccatcatgct ctcggcgccg
     8941 ttatagcagg tcagcagcgc cacgggccgc ccccggcgca ggtagtggat cgcgacggcc
     9001 atgctgtccg cgccgccgga aaagagaatg gctgcgccgg gtcgtttctt catgccgtct
     9061 ccatgccgat acaagccgca cgaaacggtc tcgcaggagc tcgaccctcc aagggtttga
     9121 ctacgccgtg gagggcggag ctccgcgacg ccgcgtcagg caggcaagtt cttgaggtcc
     9181 accagcttgc cgtggctgat cacgtgcagc ggctcgcggt gataccatag ggcctcgtat
     9241 acggtcgggg cgtcgagcac gacgaggtgc gcgggggcgc cttccttgac cgcgaagttc
     9301 ttcacgttga tcgccttcgc cggcgccgtg gtgatcatgt cgtagatctt ttccatctcc
     9361 gggaacgtgg tcatccagag caggtggacg ttcaggaacg cgacctccag catgctgttc
     9421 tggccgaacg ggtaatacgc gtccgcgatg tcgtcctggc cgaggcacac gaggttgcct
     9481 tccgccagca gctccttgac ccgggcgtgg agcgggcccg tgtgcgggtc gctgacgacg
     9541 cccatgccgg ccttgcgcag cagcgccgcg accttgcgga agtacggctc gggatacatg
     9601 cacatcgccc gcgcgtggtg cgccagggag cggccgatcc gcccggtctc aatggtcttg
     9661 agggcgagca tctccagcgt ccgcagcccc ggatcgcccg cgtcgtccac gagcatcgag
     9721 aggtccgcgt cgtaggcctg cgcgatcttg gccatctcgt ccacgtgctt ctgcgagtcg
     9781 gcgttcgtgt actcgatcca cgggatgccg ccgacgacgt ccgcgccgag gtccatcgcc
     9841 tgccggatca ggtccgccgt gcccggctcg cggacgacgc cgtcctgcgg gaacgcgacg
     9901 acctggacgt ccacgatgcc cttgaacatg tcgcgggcct tgatcaggga cttcacgccg
     9961 atcagcttgg ccttcgagtc cacgtccgcg aacgcgcgga tgtgcgtgtt accgttgcgc
    10021 gccgcgtgcg cgagggcctt gaggacgttc gggacgatcc acttctcgtt gtacttgtcc
    10081 ttcacgcgcg cggccagctc gatggcggtc atggcggcgc ccatgctctc gccgtggtag
    10141 gcggacagcg ccttggtgtc ctgcaacgcc agcgtgtaga ccttgcacag gtgcaggtgg
    10201 gtgttcacgt acgattccgt caccagccgc ccgccggcat cgatgacctt gcccgcccgg
    10261 cccgcgatct tcgcggcgat cttcgcgatc acgcccttct cgatcgcgat gtccaccagc
    10321 cccttcttcc cccgcaactg cgccttccgg atgatcagat cgtacgccat ggtattcctc
    10381 ccttttcttg tttcgttgtt taagcttttg ccgctttctt caggtatccc cgcgccagtt
    10441 tggcgccctc gaagatggcc tcgtagcccg tgcagcggca gaggtggatc tcgagggcgt
    10501 ccacgatctc gtcgtccgtc gcgtcgggct tcttaccgaa cagcgcgtag agccgcagga
    10561 cgatgccggg cgtgcagtag ccgcactgga ccgcgccggc gtccacgagc gcctgctgga
    10621 tcgggtgcag tttcccgacg ccgcccgaga ggccctcgat ggtgacgacg ttcccgtcct
    10681 tgtacttggc caggccggtg atgcagctct gcacggcctc gccgttgagc acgacggtgc
    10741 acgcgccgca ggtggcgtcg tcgcagccac gcttggcgcc ggtgaggtcc agcttgtccc
    10801 gcaggacatc aatcagccgt tcccagccct cgatctccac ggttcgcggc acgccgttga
    10861 cggtcaggtt gattttttca gccatggtcg tgtcctcttt tcaattcgtt ttcccacagg
    10921 tagggcggct tggctcaagc cgccgtttac gcggcgcgtt cggcgaacgc gccctaccgg
    10981 ccctccagcg ccttcttgac cttgtccggc gtgaccggca actcggtaaa ctcgacgccg
    11041 atcgcgtcct ggatcgcgtt gaggatgcag ggcgcgacgc cgacgagcgg gtgctcgccg
    11101 atgccccgcg cgccgaacgg gccgaccacc gccggggtct cgacgcagac caccgtctgc
    11161 ttcgccggcc ggtccttgat ggtcgggaac cggtagcggc cgaagaacgg gttcaggcac
    11221 ttgccgtcct tgtcgaactt cagctcctcg tgcagcgtcg aaccgatcgc gatcatgacg
    11281 ccgccgacga tctgcccgcg gatctgcttc ggctggatca cccggccgac gtcgaacgac
    11341 gaggcgaagt ggtcgatcag gaccttgccc gtcgccttct cgatccggat atcgcacgcc
    11401 tgcgcgccga acgtgtactc cacacccgcg ctgccctgcc cggtctccgg gtcgagcccc
    11461 gagtagcgcg gcagccggca gtccgacgtg gtctggacga cctcgccgat cgtgatgccg
    11521 tcgccggtga tgtagccgtg gatcagccgc ggcacctcga cgcggacgcc ggggtcggac
    11581 ttgaggaaga cgaactcgcc gtcgtagtcc agcatgtcct cgtcgcactt cagcacctgg
    11641 ccggccgtgc gcttgaggat ggcgatcgcc ttctcggcgg cccggacgat cgcgcggccg
    11701 ccctgcgtgg tgaacatcga gccgatggtc tgccactccc acggcgagaa ctgggtgtcg
    11761 atctccgtgt agacccggat gcgctcgggc gggatcttca gcgcctcggc ggcgaccagc
    11821 cggacgacgt tgcgcaggcc ctgccccacc tcggcgccgc ccatgttgat gcagacgctg
    11881 ccgtccgagt tcatcttgag gtagcagccc ttcgaggaga acggcgcgcc cttgggcgac
    11941 ttcatcaccg ccacgaaccc gcggccgtag tagaactgtt cgtcctcctt cggcttgggc
    12001 gagtcgaaga cggccttctt cacattcgcc acgcaggcgc ggacgtcgcc gttggtcttc
    12061 cacagcttct cgcccagcga ggtggtcttg ccctcgccga ggtagttctt ctcgcgcagg
    12121 gcgaagggcg acatcttgag cttgcgcgcg agcatgtcca tcagccgctc gacgccgaac
    12181 tggccctcct gatggccgta gccgcggaac gcgccgatcg gcggcgtgtt ggtgtaggtc
    12241 aggtacgact ccatgaagca gttcggaaac tcgtacggcc cggtgcagtt gtgcgtcgcg
    12301 ccgatgcaga cgtggatgcc cgtgtcggcg taggcgccgg tggagtggta cagcgtggcc
    12361 tggatcgccg tcaacgtgcc gtccttcttc gcgccgagct tcatcttcgt gcggatgccg
    12421 tggcccatca gcgtgctgac caggtcctcc ttgcgcgtgg ccacccactt gaccggccgg
    12481 cccggcacga agctggcgat gaacgcgatg gtctgctcga ccgtgatgtc ggacttgtag
    12541 ccgaagcacc cgcccacctc gggaatatgc acgcgcacgt cggagatcgg ccggtcatag
    12601 acccgcgcga cctcgtcgcg gatgacgaac gggcagatgg acgacgacca gatctcgatc
    12661 agcccgtcct cgtggaacca cgcgatcgcc ccgtgcggct cgatggccga gcaggagccg
    12721 aacgggtagt tgaactcgcc ctcgaccgtg acctcggcgt cggcgaagcc cttcttcacg
    12781 tcgcccttca cgagcttgta gtagttcgcg atgttcgtcc cggccttcgg gccgaggccc
    12841 ggcaggtgcc agaagtcgcc gctgtcggga tggatcagca cggcgtcggg cttgagcgcg
    12901 tcgcgggcgt cgacgtagac cgggagcggc tcgtagacgg gcttgatctt cttcaccgcc
    12961 tcgcgggcgt ggtgggcgct gtccgcgatg accgccgcga cggcctcgcc gatatggcgg
    13021 acgcgatcgc cggccatcgg ggtgaagtcg cggatgttgt cgccgtagcg gtagttctgg
    13081 ccctgggccg tgaccacctt gaccacgccc gggcacttct cggcctccga cgtgtcgatg
    13141 gacacgatct tcgcgtgggc gaccgtcggc cgcaggatcg cgcaatgcag catccccggg
    13201 agcttgaggt ccgccaggaa cttggccttg ccggtgatct tggccgtcgc gtccggccgc
    13261 aggatatcct tcccgatgta cttgaattcc atgggactcc tccaggcttt atcaataggt
    13321 ttggagatag taccccacgc ccgcggcccg gtgaataata aaacgggcct tttttacatt
    13381 tctggccggt ccgggtactg acttggacat ggggagttcc cggcttgccg gggcaaaacc
    13441 ggtggcctag aattacacaa tatgacccgt acaacctgtc tgttcacgct ggccgtgtgc
    13501 gccgcgctcg gcctgtccct gcccggccgg gccgtcgccg gagaccccaa tccggccacc
    13561 ccggaagacg agagcgccaa ctggacggtg accgaccccg tggaccagga tttctcggaa
    13621 gggacgccgg cgccggcgtc gcgggaggcc gccccggccg tcctgcccgt atccatccac
    13681 caggccgagg ccgagtacta caagcagttc cgggccgtca cggaaagcga ctgggacgcc
    13741 gtgcggcgcg acctgcgcga gaaggaactc ggcgccgctc cccgcgcgct cacgggcgcc
    13801 tcgacgcccg ggccgttcac cctccagaag gaggtcttcg gctggtaccc gtcgtgggaa
    13861 ggctccagct acaccaactt cgagttcacc ctgctctcga ccgtcgccta cttcaattac
    13921 gaggtcaacg cgggcaccgg ctacgcggtc acgatgaaca gctggtccaa cacgccgctg
    13981 gtctactggg cgcattccaa caacgtggac gtggtcctct gcgccacgct catgtcgaac
    14041 cacgccgcct tcttcggcag cgccgcggcc cagagcaatc tcatcaacga gctcgtccgc
    14101 gtggtccagt tgcgcggcgc ggaaggcgtc aacattgatt ttgaggccat cgccagcggg
    14161 tacgccgagg aactgacgtt gttcatgagc aacctgacgg tcaggatgca tgcccaggtg
    14221 cccggctcgc aggtctccat ctgcctcccc tccgtggact ggagcagcaa tttcgacgtc
    14281 gccgagtacg aggggtttct cgataagtgc atcatcatgg gctacgatta ctcgtggagc
    14341 acggatgatc aggccgggcc ggtcgcgccg ctcaagggca gcgccatctt cagttcgtgg
    14401 tgcgttcaga aatccgtcac caactacttg gacctcgggc tctcgccagg gaagctgctc
    14461 cttggctgcc cgtactacgg ttacgactgg ccgacggtct cccacgcgat gcacgccgac
    14521 accacgggcg acggcaccgc ccggcagtac cccagcgcgg agtcgtacgc cggcacctac
    14581 ggctacaact gggacacgaa ctcgctgacg ccctacgtgc tctacggcag ctaccgccag
    14641 ctctggtacg acgacacgaa cagcctcggc ctcaagtacg acttcgtcaa tatgaaggag
    14701 ctggccggca tcggcatctg ggcgctgaac tacgacgagg gcacctcgga tctctgggac
    14761 ctgctcgcca gcaaattcgc gggcgaggcc gactggcaaa gccaggaccc ggggacgacg
    14821 gcctccttct acggcgtgga cgccctgaac ggccagtggg ccgccgtggg cgcgggcggg
    14881 gtgatctcca cctccggcga cgacggcctg aactggacct cgcgcgtctc cggcacgggc
    14941 agcctgctgc tgaacgtcgt cggcgggggc ggcctgtggg tcgccgtcgg ggacacgggc
    15001 acggtggtga cctcgtccgg cggggcgacc tggacgacgc gctccacgcc gaccaacgcc
    15061 atgctgcgcg gcgccgccta cggcaacggc gcgtacgtga cggccggcgc cggcggcgtc
    15121 atcctgcgct cgacggacgg ctccacctgg gcgcagcagg tctcgggcac aacgaacacg
    15181 atccagggcg tgaactacgt cggcagcctg ttcatcgcca cgggtgaaaa cagcgtcctg
    15241 ctcacctcct ccgacggcat ctcctggacc tcgcgcacct cgaacgcggg cggctggctg
    15301 ctggacgccg cgtacggcaa cggcgcgtac gtggccgtgg gcatcggctc gcgcgtcgtg
    15361 acctcgccgg acggcacgac ctggacccgc cagaccaacg acctcccctc ggccagcgcg
    15421 aacctctacc gcgtggcctt cggcagcggg caattcgtgg ccgtggggca aggcggcgtc
    15481 atctggggct cgacggacgg aagcaactgg accgccgaag tatccggcac gacgaatttc
    15541 ctccgcggcg tcgcgttcag caacgacctc ttcatggcgg cgggctacaa cgggaccatc
    15601 ctgaccaagg gcgcgggcgc gccgtccgtc gagatcgcga ccgcgggcgc caccgtgccc
    15661 aacgccacct ccagccagac cgtcaccggc gcggccaacg cctacgtcgt gggcacgatg
    15721 cgctggacca acagcctggg cggcagcggg acgttcgccg cggcgactcc ctggtcgttc
    15781 tccgcctcgc tctcggtcgg gaccaacgtc ctcaccgtca ccggcacgaa tacggcgggg
    15841 tcggcggcca gcgacagcgt gtccttcatt cggcaggcgg cggagggcgg cggcggctcg
    15901 ggatcggaaa cgacggccgc ggcgcagccg ggcggcgcgt tgagcgacgt cgtcgtcttc
    15961 accagcgcgg gccacgggtt cacgtacaac agcacctatg ggtggatcac gggccgcggg
    16021 ttggacaacc aggtggtcga ggacatcggc aacatcgacc agctcaacta tttcgccgag
    16081 tactgcttca aggccggggc caccgtggtc cccttccgcc ccttgggcca gcagaccaac
    16141 gaggtggtgc tggacaacac caactccgcc cgcgtcacct tcggcgggac ctggagcaac
    16201 agcacctcca gcacctacta cggcgaggcc ggcgccacgc cctaccgtta cgcgtacatc
    16261 aactcgaccg cgaccacggc ctgggccatc taccggccga accttcccgc cgccggcttc
    16321 tacccggtct acgcgtgggc gcgcagcggg tcggaccgcg tgaagcagct gtaccgggtc
    16381 taccactccg gcggcgtgac ggacgtgcgg gtcaaccacc ggcgcgtggg cctgggctgg
    16441 gtctggctgg gcacctacta cttcgacgcc gggaccaacc atagcgtgaa catctcgaac
    16501 tacgcgcccg acgggtacct ggccacggac gtggtcatcg ccgacgcgat ccggttcggc
    16561 aacggcatgg gcaacatcga ccgcggcgcc ggcgtgtcgg gcctggagcg ggaactggag
    16621 gcctcccggt actgggtgca gtccatggtc ggacagggca tgagttccac cctgtacgac
    16681 agcgccggcc tcgacgacac cgacgacaac gtcggcgcgc ccaaccgcat ggcgtacgag
    16741 atgaaccgcg agtccgacgg cgggtatttc gaccgcatct atctcgggtt ccattccaac
    16801 gccggctcgg gcgccggccg cggccccatg ggcctctacg acacgcgctt ctcggcgccg
    16861 tacagcggca tgcagcaggt ctacgccacg aaggtcgccg ccgaactgac caacgacctc
    16921 gggtacggcg acctaggcgt ctggttcccc gacagcttca ccaacaacac ggcgaacctg
    16981 tacgggagca tgtacggcga gatattcggc gccatcgtct cgaacgagat gaactcgacg
    17041 atcatcgagg tgggctacca cgacaacctc catgacacgt atctcctccg ctgcccctcc
    17101 gcgcggcgcg tgatggccat ggcttcgtac caggccattg tcaagtactt gagcgcgagc
    17161 aatgccgctg tttcctccgt cctactgccc gatccgccca ccgacgtgag cgcggtcaac
    17221 gacggcgcgg gcgccgtgac ggtggcgtgg caggccccgg taaccaacaa ggcgggcggt
    17281 tcggcggcaa ccgggtacat cgtctaccgc tcaaccaacg gattcggatt cggcaatccc
    17341 gtcacggtgc ccggcgcggc cacgctgtcg gcgaccctga ccaacatgac ggcggggcag
    17401 atctactact tccaggtgtg cgcgaccaac gcgggcggcg agtcgctggc ttccaagacc
    17461 gtcggtgtgc gcgtttcgcc cgcgggctac gcgacgcact tggtcgtcaa cgggttcgag
    17521 cgcaacgagc ggcgcctgac gcccacgcgc tactacgcct acaacatcga cggcgacgtc
    17581 gcgcgcgtga tccagcgcac gatcaactcc caggattacg tcatccaata cgggaccgcc
    17641 ctcgccgccg cgggccgcta cttcgactcg tgcgacaacg acgcggtcgc cgacggcgac
    17701 atcgacctca ccgactattt cgccgcctac tggatcctcg gccgcgaatc gaccgccgac
    17761 gagaccatct ccgacgcgga acaggccctg gtcacggcat tcctgaacca aagcaaccgg
    17821 ctgttcgttt cgggtactga actcggttgg gacctgcaac agtacggcgc cgcctcggat
    17881 aagagcttct gctccaacta cctcaagacc gcctacgtgg cggacgatgc gggaaccaac
    17941 gtggccgccg cccgcgccag cggagtattc ggcggtctcg cgaacctggt gttcgactac
    18001 accggcacgg gccccgtcta ccgggccgcc tacccggaca cgctggccac ggccggctcc
    18061 cccgcctcca cggccgcgct ggtctacggg acgtccgcgg gcggcgccag cgtggcggcc
    18121 atctcttaca gcaaccagta tcgcctcgtg gtcatggggt tcccgttcga gaccatcctg
    18181 gacgcctccg cccgcaccaa cctgatgagg cgcacgctgg acttctttgg cagcgcgccg
    18241 gcggacagcc cggtgacgga catcaccaac ggcaacgcgt ccgtggccaa cgccgtcggt
    18301 tccatcgtga tcggcggcac gaacaacccc gccgtcatcg ggcagatgac ctgg
//
LOCUS       DAOOTK010000055        18250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2100_length_18250_cov_4.894751, whole genome shotgun sequence.
ACCESSION   DAOOTK010000055 DAOOTK010000000
VERSION     DAOOTK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18250
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_18250_cov_4.894751"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK634_07820"
     CDS             <1..371
                     /locus_tag="PK634_07820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOW97680.1"
                     /translation="HRVINGFMIQGGGFTRNMQQKPTRGPVRNEADNGLKNARGTIAM
                     ARTSDPHSATAQFFISVVDNASLDHTSPDPRGYGYCVFGRVVEGLDVVDKIKAVQTTQ
                     RGPFADVPAEPIEIVEAKRQ"
     gene            454..1455
                     /locus_tag="PK634_07825"
     CDS             454..1455
                     /locus_tag="PK634_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOW97681.1"
                     /translation="MASNKNKRYWVGFDLGGTKMMATVFNEGFRPLASEKVKTRAQSG
                     PRQVLSRLLDTIQAAVGQAHLKMSEIAGIGVGCPGPLDLDRGVILTAPNLGWRNVPLK
                     NTLEKRFGCAATIANDVDAGLYGEFRFGAARKARCALGVFPGTGLGGACVYEGKLFRG
                     KKGSCMEIGHIPVERGGRLCGCGQRGCLETVASRLAIAAEAAMAAHRGEAPHLLGLAG
                     TDVGAMKSGTLAKAIEAGDRVVEQIVRNAAQKLGGALAGVVNLLAPDVLVLGGGLVEA
                     MPNLFLGEVRKVIEDKAMKMYADDLKIMPAKLGDAATAMGAAALVADMIKAREEEDA"
     gene            1452..3065
                     /locus_tag="PK634_07830"
     CDS             1452..3065
                     /locus_tag="PK634_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOW97682.1"
                     /translation="MSPDPVPAPESPAPPRNVRMAAVIDIGATAIRMEIAEIDNQGGV
                     RKLDSLSHGVQLGKDTFTRGRIQQSTIQECVDVLKGFRRVMDEYGITKPEQVRAVATS
                     SVREAQNRDTFLDRIYITTRINVRAIDEAEENRLTFMAAQTVLADEPELETGSLLVAD
                     VGGGSTELLLLQEGHIVFSDSYRLGSLRMRETLETHRATTERVRTILGQHIQRLVEQV
                     VRNLPVSTVPVLVAMSGDAHFAAAQLCLDWSERKTARLDYKALAGLNDKLLPTSVAKL
                     VGKYQLTYQEAETVGPALLVYQHLALAFKAEHILVPKASFRDGLLKEMTLRGYWTGSF
                     ADQVRHSALALAEKYNVDAAHAAHVADLCVRLFRELQPEHQLDQRYELLLEVAARLHE
                     VGGFISSRSHHKHSLYIIQHSDLFGLTREDLAIVALVARYHRRALPAATHPEYMALGR
                     DDRITVSKMAALLRVADALERNHLQQVRDLDFAREPGQFVIRVRNVEDLTLERLALKE
                     KGGLFEEVYGMPAVLKEERFEDAEARDGA"
     gene            3055..5169
                     /gene="ppk1"
                     /locus_tag="PK634_07835"
     CDS             3055..5169
                     /gene="ppk1"
                     /locus_tag="PK634_07835"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOW97683.1"
                     /translation="MAPKADRFFNRELSWLAFNRRVLEEAQDASLPLLERLKFLAITS
                     SNLDEFFMIRVGGLQQLRAQGWDGADASGLTVKEQLAEIGQAVRGLMADQYACLLNGL
                     EPRLAEQGIRRMDRGSLTPEQDGYVEAVFEHEIFPIITPMAVDIEEPFPLLPGLGLNL
                     CVRLKAVRGAELPRFAVIPLPRGLARFVTVPTDAGFHYLPIEDLVTAYLDRVFPGEPI
                     LECIPFRITRNADLSVAEDAASDLLSQMKEVLTERKRSPCIRLEIGDKPSDILLGFLR
                     SALDLSDQEVYPAPGPLDLAAFYRLASMTGFDHLKIEDWPPQAPPDLPPQEPMVDVLA
                     RRNVLLYHPYDSFDPVVRFIEEAVNDPNVLAIKQILYRTSENSPVVAALARAADRGKH
                     VTVVVELKARFDEARNIGWATALEHAGVQIIYGLKGLKTHAKICIIVRREPGGIRRYV
                     HFGTGNYNEKTARLYSDISFMTSDEDYGADASSFFNTITGYSQPVRYRKIEAAPIGLR
                     DRLLDLIQNEIERCKQGQPARIMAKLNSLADPELIQKLYEASKAGVKIELNVRGICCL
                     RPGVPGLSDHITVISILDRFLEHSRLFYFHNGGEELVFISSADWMPRNLDRRVELLVP
                     VEDPEGRARLVSILETSLQDNVKARRLRPDGTHERLSAPAKQKAVRSQEVFYRRAVET
                     ARAAASASGPVFEPHRPAGAQP"
     gene            5171..6055
                     /locus_tag="PK634_07840"
     CDS             5171..6055
                     /locus_tag="PK634_07840"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOW97684.1"
                     /translation="MNAPGIQPSESAAEFLRRHILAQAEVIRRTEDGVRAGREGPLHD
                     MRVAIRRLRTLLRAFRKPLAETRAAAVEEGYARLLKELGPARDTDVWMLFLKRHPVLR
                     RGASARFLAAQKREKARRQQQVRERLESAENGALKGALARLIDEDLKPGTLRVQPNPA
                     ADLGRRALRKALKRIHRRARGLPRLSPEKMHRLRIACRKARYVAEFLSGAWDAPLDAL
                     ARRFKAIQDVLGDVHDQDVYLAHLRTCRPRPPARMLRSIHLQRDRDRLRFEEDWARFV
                     KPRYQRRLDRILSPRRSP"
     gene            6175..7887
                     /locus_tag="PK634_07845"
     CDS             6175..7887
                     /locus_tag="PK634_07845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOW97685.1"
                     /translation="MSRARDTASAAPGVEASAGKYPGKGFHIGEYVITDFLGHGSMAS
                     VYLAVDGTGHEVALKVFQEGPGVSATMLERFRREAEASKKLRRHPNIMPIYGTGRDGP
                     YHYIAMESIRNSRTLEAALESTPMSLLRVVEIAIRIARALQYAHTHNIVHRDVKPTNI
                     MIDEFEEPLLSDFGVAELMDWPSCTLTGALTGTPLYMSPEQARGERVGPASDIYSLGV
                     VLYEAVTGVMPYAIQHGSPVKDVLEAVKNELPRRPRLFRKEISPDLEAVLFKALEKEP
                     RDRYVDAEAFARDLERVQAGRRVSAHRFSILAYLRHLARRHQQGLLIFLAMAALLSGA
                     GLLFRQRLLRTRFEEVVRRAQLKNALYMLAEAENGAGFERETPRAWNDIRLARKAMIG
                     SDWSAARDGFRAAADISRSVGDTRTAAIAELDQARCEIMLQDRAAAQALYHRILTNPD
                     ASPAIANMAQMESIELALLAGDRPRAFEVLMLRTPPAEGPIRTAIKGLAGDLSSRAVA
                     ESVERMQHPFQNDAYVAAAIRAYLDGEIRLAVGYMRRAIELSSPPSEWPAPFARQLFN
                     EVDR"
     gene            7890..8432
                     /locus_tag="PK634_07850"
     CDS             7890..8432
                     /locus_tag="PK634_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOW97686.1"
                     /translation="MAPHPPDPGRARTHRRRGFTLIELLVVVLILAIGIVWTVHHVRQ
                     QRLTRRCAEQLWHIYTALEMYEIDRGTLPRLAFFPNDPKQDADSLFTVIQPYTASEGL
                     TVCPALPPAQRALGLTYVWNVENNGRKLHPPGVARWMLVEISALSDSVPPPHEGRYHI
                     LYSDGQVELSENPPPDLRRL"
     gene            complement(8442..9278)
                     /locus_tag="PK634_07855"
     CDS             complement(8442..9278)
                     /locus_tag="PK634_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOW97687.1"
                     /translation="MHRVINHLIQLQELRVARAQQEASMQGARLSQLDASIQTLVEQL
                     PPEIAARYQKIEKKGLLAIVPVANGVCSACGMSLPVSLVHAVHAADSLHHCPNCARML
                     YYPELLPRHIGRRKRRSEPAKVGIDRFSSVDLMIPQLVSQERDEVIAEMCQKMEAEGF
                     VDDAGRLQEEALKREAIISTAVEHGLAFPHVRGVEGGGLTLALGLHRKGIRFGGPGRA
                     LTRIVFFMVIPTAASAFYLKLLSGLAQTFQEEDARDRLFEAETPEQLWKALVKATKST
                     IQ"
     gene            complement(9485..10069)
                     /locus_tag="PK634_07860"
     CDS             complement(9485..10069)
                     /locus_tag="PK634_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOW97688.1"
                     /translation="MVATPSTMLPLGTPAPDFSLTDTVSGKPVSLASFAGRRALLVMF
                     ICRHCPYVRHVEREIARIGRDYAGRDVGMVAISSNDADRHPDDAPAQLKEMAGELGFA
                     FPYCYDESQAVAKAYTAACTPDFFLFDDGRRLAYRGQLDDSRPGNGRPVTGRDLRAAL
                     DAVLAGRPASADQKPSLGCNIKWKPGNAPAYFGS"
     gene            complement(10133..13375)
                     /locus_tag="PK634_07865"
     CDS             complement(10133..13375)
                     /locus_tag="PK634_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOW97689.1"
                     /translation="MRKGLLRALGGLLLVAAGGLALLQTPWAKDRLAAVLARRWSAPG
                     WRVSLGRLDGRLPFELRLDEIRLSDAGGEWLQAAGVRLRLSAGELCAGRLVARELSAR
                     TVHVLRRPELPAAEPTSATRKTGPPRWPMAVDHLRVEGLALEEPVAGARLRLRVLGRA
                     SWDPVAGFAGAIQGERTDALPGAAHATLAWSRSKGWRLGVDLLDAVGGPLARHLPAKP
                     TGPLRARVGARGPLESCQGTLEADAAGAGRLGGSFTLDARPPLAVSFHAELENDGLLR
                     RDVERVELDGALRRLLEQPEGEMAARVQRGEDVVVARAAVRYADRRVEIRDLDARCGA
                     LSVTGGLEFDPAARLAKGRLSAELPYLSDAAARLGWPVGGEARLDVDLDPSGGEQRVR
                     ASGRVRNVTTPWASAEELRMEADMCPLGPDPTGRVEVVARDVGAKRASISNLAVRVDG
                     SRSACDWSVRADGRLARPFELAAAGRWDASEPGTVLTVREAYGRCGPGDFVLDEPARV
                     EWGDGWIAWQPLKGHVGAGRFRTAGSITGGMIRAMARLEGVPLGVIADLGGPPMEGRA
                     EGAIQLQGQVDDPAAELQLNLSAVRPGEALLIPLKPADAELRVCLADGVLRANWAVSG
                     PVQGSGSVGIPFRWSLRPWSLEVRPAAPLDGRMDADVDLARLVPASYLADRVIRGKLH
                     AGLTFGGSLEAPDIEGALALQNGYYENLDLGVVLRDVDIEARSDLSSLTITRATASDG
                     GQGKIALTGRLDYAPARHFPYQVAIRFEDTALAREDHFRLDADGEVQLDGSLAGNRLS
                     GRIDVRELDYESVDRPAASIPQLDVAEINGPERAGEAAAARSLTPALSLDLDIRAPAR
                     VYIRGRGLESEWKGQLRIRGTAAEPALAGSFSVLRGRFVFLGRRFNLESAAINLDGRS
                     PPDPQLDIRATVETGRLTARLDLAGSLSAPAWTLTSDPPQPEDEIMAQLLFNREADRI
                     TALQALRLAYGLNLLHGGGATLDVLGQGQNLLRVDQLDIRQDAEDPALTSIAVGKYVG
                     DRVYVEGEKSLTGQADAVTMELELSRSLRLQTISSPQLREGLRLKWGRDY"
     gene            complement(13395..15191)
                     /locus_tag="PK634_07870"
     CDS             complement(13395..15191)
                     /locus_tag="PK634_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HOW97690.1"
                     /translation="MKVGRPAWPGRICWRTAILLLAARTVLGAETVPPCDVRIEGAPG
                     RALRKELLSVTETWLLRHKPPPTQRLLLRRAEKDVVPLQAALRARGYYAATVEPAYSR
                     HRQKPRITFRIRPGPVYLIRSVSVEAVTGAPPESVAVPSPARLGLVARRPLLAQEVLD
                     AEAQVLRAFQAGGYPAARLAGPAVTVDHAGRVADVRFRVEPGPRVRFGPVRIRGLNRL
                     RESFVRGLIPWREGDWYDIRRVDRLRDRLALSGPLSAVEIDAGRAAADGDEIPVALIL
                     KERKRRRVELGVGYATDGGADARASWLHRNLFGGAEQLELQLGVSESLLFAEASFRRP
                     QFGRPDQDLLLDLKAQSESAEAYTSQWVRAAAGLERELSPKLRAQGGLSLRYSEVEQE
                     EREDYFLWALPARLIRDTSNDLLDPTRGTRLSFLGAPTYDLLNGEMAFFRAQVSAAGY
                     WRLARQPLLVLAWRFAAGSLLGPSRGDIPADERFYAGGGGSIRGYAYQEVGPLDEGDD
                     PLGGRSLLESSVEMRWRATRSMGLVLFADGGSAFESNVPDFDENYLWGAGVGWRYYTS
                     FGLVRVDVAVPLDQRPDVDEDYQFYVSIGQAF"
     gene            complement(15246..17216)
                     /locus_tag="PK634_07875"
     CDS             complement(15246..17216)
                     /locus_tag="PK634_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97691.1"
                     /translation="MNETSPQAGEQKQGGGWLAWVGLAVLALWGIRTIGHSSIWLRLA
                     TGRAIAEQGIPRADAFTFTAAGLPWVNTSWLYDRLLYGLWNLGGAALVTLLHAAFVVL
                     AFGLLIRPARRLAGPVGTALALLGCAWLLSVRFVVSPVLPSLLFAALFIRVLSESPRA
                     WMPVTLLPVQWLWANVHGSFVLGPILFLLFAIERWHGNRESQDKKAVGWAAAMIPAAL
                     VVSLLNPFGAAVFTQTFRDWSLVVFAYVQEWISPFSLQFQRAFFVKNIVTLALLIGAI
                     GLITEKRKLPIAMTSLAVLGAFLVVRSLRHVEFFALLAFPFLALSGSAAGAYLGDRVR
                     PALGTRAAWWSRAGALLVLLLAAGSAAAVLSNHFYESVGSPSVTGFGVASGLYPEKAA
                     EILAKPGFPAKTVHLFQDGNFLLWAAPGRPVFVDQRADLHAPEFFEAVARSLNGEAEA
                     WDALEKKWEPESVLLNGCALGAGPLLRMLLESRRWALAYLDGTSALLIRPTAANQAWL
                     SNEEIQKAGLLVLEQERREYQRRMACCARPVVSPRLIGAGTFYLALDRFPEAELVYDL
                     LTRGSPAMVSAWYSLGMVRMQLGKFDLAATALEQACRRRPRDPMFWLQLGRAYAGAGM
                     AQDAERAFEKGRKLNPALAERFRSGMEAPAAP"
     gene            complement(17297..18184)
                     /locus_tag="PK634_07880"
     CDS             complement(17297..18184)
                     /locus_tag="PK634_07880"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOW97692.1"
                     /translation="MLHDRRSLDLAGGGPFAAALDQAAAMGGTAWVLTFDPHPMKILR
                     PREAPLSLTSTPHKIRLLHELGIEGCLVLAFTPELAALDPGPFLARLTGAWPGARQLV
                     VGSNWTFGHRGRGNADLLRKWVAAQGLQATIVEPVLWDGLAISSTRIRRAVQEGRLDD
                     AAVMLGRPYSLLGTVMAGRRVGTRLGFPTANLDPHNEALPPPGVYAVRAEMKDGAWTG
                     AAFLDSKPHEGLPAGVNLVEVHLLDFSGGELYGRDMEIRFGRRLREVRRFDSLESLRV
                     QIALDVEQVRAEAAGGPGP"
BASE COUNT         2942 a         6546 c         6273 g         2489 t
ORIGIN      
        1 tccaccgcgt gatcaacggc ttcatgatcc agggcggcgg cttcacccgg aacatgcagc
       61 agaaaccgac ccgcgggccg gtccgcaacg aggcggacaa cgggctcaag aacgcccgcg
      121 ggacgatcgc catggcgcgc acctccgatc cccacagcgc gacggcccag ttcttcatca
      181 gcgtcgtgga caacgcctcc ctggaccaca cgtcgcccga cccgcgcggc tacggctact
      241 gcgtgttcgg ccgcgtggtc gagggcctgg acgtggtgga caagatcaag gccgtccaga
      301 cgacccagcg cgggccgttc gcggacgtcc cggcggagcc aatcgagatc gtcgaggcga
      361 agaggcaata gtaggacgcg ctggcccagc acaccgcggc ggcttgagcc aagccgccct
      421 acccggacgc gcccgaaaca aaaaaggact cgcatggctt ccaacaagaa caagcgctat
      481 tgggtcggct tcgacctggg cggcacgaag atgatggcga cggtcttcaa cgagggattc
      541 aggccgctgg cttcggaaaa ggtcaagacc cgcgcacaga gcggtccccg gcaggtgctg
      601 tcccgcctgc tggacaccat ccaggccgcc gtgggccagg cgcatctcaa gatgtccgag
      661 atagcgggca tcggcgtggg gtgcccgggc ccgctggacc tcgaccgagg ggtgatcctg
      721 acggcgccga acttggggtg gcgcaatgtg cccctgaaga acacgttgga gaagaggttc
      781 ggctgcgcgg caacgatcgc aaacgacgtg gacgcagggc tgtacggcga gttccggttc
      841 ggcgcggcgc ggaaggcccg ctgcgcgctg ggcgttttcc cgggcacggg cctgggcggc
      901 gcctgcgtct acgaggggaa gctgttccgg ggcaaaaagg gctcctgcat ggagatcggg
      961 catattccgg tcgaacgggg cggccggttg tgcggctgcg ggcagcgggg gtgcctcgaa
     1021 accgtcgcca gccgtctggc catcgcggcg gaggccgcca tggccgcgca ccggggcgag
     1081 gctccgcacc tgctgggact cgcgggcacc gacgtgggcg ccatgaagag cggcacactg
     1141 gccaaggcca tcgaggcggg ggatcgcgtg gtcgagcaga tcgtgcgcaa cgccgcgcag
     1201 aagctcggcg gcgccctggc cggcgtggtg aacctgctcg cgccggatgt cctcgtcctg
     1261 ggcggcgggc tggtggaagc catgcccaac ctgttcctgg gcgaagtccg caaggtgatc
     1321 gaggacaagg cgatgaagat gtacgcggac gacctgaaga tcatgcccgc gaagctcggc
     1381 gacgcggcga cggccatggg cgccgccgcg ctcgtcgccg acatgatcaa ggcgcgggag
     1441 gaagaggacg catgagcccg gatcccgtcc cggcgcccga gagccccgcg cccccgcgga
     1501 acgtccgcat ggccgccgtc atcgacatcg gcgccacggc gatccgcatg gagatcgccg
     1561 agatcgacaa tcagggcggc gtgcgcaagc tggacagcct ctcgcacggc gtccagttgg
     1621 gcaaggacac gttcacccgc gggcggatcc agcagtccac gatccaggag tgcgtggacg
     1681 tcctcaaggg attccggcgg gtcatggacg agtacggcat cacgaagccg gagcaggtcc
     1741 gggccgtggc gaccagctcg gtgcgcgagg cgcagaaccg ggacaccttc ctcgaccgca
     1801 tctacatcac gacgcgcatc aacgtgcggg cgatcgacga ggcggaggag aaccggctga
     1861 ccttcatggc cgcgcagacc gtcctggcgg acgagcccga gttggagacc gggtcgctcc
     1921 tggtcgccga cgtgggcggc gggagcacgg agctgctgct gctccaggag gggcacatcg
     1981 tattttcgga ttcgtaccgg ctgggctcgc tccgcatgcg ggaaaccctg gagacgcacc
     2041 gggcgaccac cgaacgcgtg cggaccatcc tcggccagca catccagcgg ctcgtcgagc
     2101 aggtcgtgcg caacctgccg gtctccacgg tccccgtcct ggtcgccatg tcgggcgacg
     2161 cgcatttcgc cgccgcgcag ctctgcctgg actggtccga gcggaagacg gcgcgcctcg
     2221 actacaaggc cctcgcgggc ctcaacgaca agctgctccc gaccagcgtc gcgaagctcg
     2281 tgggcaagta ccagctgacc taccaggagg ccgagaccgt cggcccggcg ctgctggtgt
     2341 accagcacct ggcgctggcg ttcaaggccg agcacatcct cgtgccgaag gccagcttcc
     2401 gggacggcct gctcaaggag atgacgctgc gcggctactg gaccggctcg ttcgcggacc
     2461 aggtccgcca ttccgccctg gccctggcgg agaagtacaa cgtggacgcc gcccacgcgg
     2521 cgcacgtcgc ggacctgtgt gtgcggctgt tccgggagct gcagcccgag caccagctgg
     2581 accagcgcta cgaactcctg ctcgaggtcg ccgcgcgcct ccacgaggtc ggcggcttca
     2641 tcagcagccg cagccaccac aagcactcgc tctacatcat ccagcacagc gacctcttcg
     2701 gcctgacgcg cgaggacctg gccatcgtcg ccctggtcgc ccgctaccac cggcgcgccc
     2761 tgcccgccgc gacgcatccc gagtacatgg ccctggggcg cgacgaccgg atcacggtgt
     2821 cgaagatggc cgcgctgctc cgcgtcgccg acgcgctgga gcgcaaccac ctgcagcagg
     2881 tccgcgacct ggacttcgcg cgcgagccgg ggcagttcgt catccgcgtg cgcaacgtcg
     2941 aggacctgac cctggagcgc ctggccctca aggaaaaggg cggcctgttc gaggaggtct
     3001 acggcatgcc ggcggtgctg aaggaggagc ggttcgagga cgcggaggcg cgcgatggcg
     3061 cctaaggccg accggttttt caaccgggag ttgagctggc tcgccttcaa ccggcgcgtg
     3121 ctggaggagg cgcaggacgc ctccctgccc ctgctggagc gcctgaagtt cctcgccatc
     3181 acgagctcca acctcgacga gttcttcatg atccgggtcg gcggcctcca gcagctgcgg
     3241 gcgcagggct gggacggcgc ggacgcctcc gggctgaccg tgaaggagca gctcgccgag
     3301 atcggccagg ccgtacgcgg cctgatggcg gaccagtacg cgtgcctgct gaacggcctg
     3361 gagccgcggc tggccgagca gggcatccgc cgcatggatc gcggcagcct gacgccggag
     3421 caggacgggt acgtggaggc ggtgttcgag cacgagatct ttccgatcat cacgccgatg
     3481 gcggtggaca tcgaggagcc gttcccgctg ctgcccggcc tcgggctcaa cctgtgcgtc
     3541 cggctcaagg ccgtccgcgg cgccgagctg ccgcgcttcg ccgtcatccc cctgccccgc
     3601 ggcctggcgc ggttcgtcac cgtgcccacc gacgcaggct tccactacct gccgatcgag
     3661 gacctggtca ccgcctacct cgaccgcgtg ttccccggcg agccgatcct ggagtgcatc
     3721 cccttccgga tcacccgcaa cgcggacttg agcgtggccg aggacgccgc gagcgacctg
     3781 ctctcgcaga tgaaggaagt cctgaccgag cgcaagcgca gcccgtgcat ccggctcgag
     3841 atcggcgaca agccgtcgga catcctgctc ggcttcctgc gcagcgccct cgacctgtcg
     3901 gaccaggagg tgtaccccgc gcccgggccg ctggacctcg cggcatttta ccggctggcg
     3961 agcatgacgg gcttcgacca cctgaagatc gaggactggc cgccgcaggc accgccggac
     4021 ctcccgccgc aggagccgat ggtcgacgtc ctggcgcggc gcaacgttct gctctaccac
     4081 ccctacgaca gcttcgaccc ggtggtgcgg ttcatcgagg aagcggtgaa cgatcccaac
     4141 gtgctggcca tcaagcagat cctttaccgg acgagcgaga acagcccggt ggtcgccgcc
     4201 ctggcgcgcg cggccgaccg cggcaagcac gtgaccgtgg tcgtggagct caaggcccgc
     4261 ttcgacgagg cgcgcaacat cggctgggcc accgcgctcg agcacgccgg cgtccagatc
     4321 atctacggat tgaaggggct gaagacccat gcgaagatct gcatcatcgt gcgccgcgag
     4381 cccggcggga tccgccgcta cgtgcatttc gggaccggca actacaacga gaagaccgcg
     4441 cgcctgtaca gcgacatcag cttcatgacc tcggacgagg attacggcgc cgacgcgtcc
     4501 tcgttcttca acaccatcac cggctactcc cagcccgtcc gctaccgcaa gatcgaggcc
     4561 gcccccatcg gcctgcgcga ccggctcctg gacctgatcc agaacgagat cgagcgctgc
     4621 aagcagggcc agcccgcgcg gatcatggcc aagttgaatt ccctggccga tcccgagctg
     4681 atccagaagc tctacgaggc ctcgaaggcc ggcgtgaaga tcgaactcaa cgttcgcggt
     4741 atctgctgcc tacggcccgg cgtgccgggc ttgagcgacc acatcacggt catcagcatc
     4801 ctggaccggt tcctcgagca cagccgcctc ttctacttcc acaacggcgg cgaggagctg
     4861 gtcttcatct ccagcgcgga ctggatgccg cgcaacctcg accgccgcgt cgagctgctg
     4921 gtcccggtcg aggatccgga aggccgggcc cgcctggtat cgatcctcga aacgagcctc
     4981 caggacaacg tcaaggcgcg gcggctgcgt ccggacggga cgcacgaacg cctgtcggcc
     5041 ccggcgaagc agaaggccgt ccgcagccag gaggtcttct atcgccgggc cgtcgagacc
     5101 gcccgcgcgg cggcctccgc gtcggggccc gtcttcgagc cgcaccggcc ggccggggcc
     5161 cagccgtgac atgaacgcgc cggggatcca gccttcggaa tcggccgccg agttccttcg
     5221 acgtcacatc ctcgcgcagg ccgaggtcat ccggcggacg gaggacggcg ttcgggcggg
     5281 acgggagggc ccgctgcacg acatgcgggt ggccatccgg cggctgcgca cgctcctgcg
     5341 ggctttccgc aagccgctgg cggagacgcg tgccgccgcg gtagaggaag gatatgcccg
     5401 cctgctgaag gaactcggcc ccgcccgcga cacggacgtc tggatgctgt tcctgaagcg
     5461 ccaccccgtg ctacggcgcg gcgcgtcggc gcgcttcctg gccgcgcaga agagggagaa
     5521 agcccgccgg cagcagcagg tccgcgaacg gctggagagc gcggagaatg gcgcgctgaa
     5581 aggagcgctg gcgcggctga tcgacgagga tctcaagccc ggcacgcttc gcgtccagcc
     5641 gaacccggcg gccgacctcg ggcgccgggc gctgcgcaag gcgctgaagc ggatccaccg
     5701 gcgcgcgcgg gggctaccgc gcctgtcgcc ggaaaagatg caccgcctgc gcatcgcgtg
     5761 ccgcaaggcg cggtacgtcg ccgaattcct ctccggcgcg tgggacgccc cgctggacgc
     5821 gctggcccgg cggttcaagg ccatccagga cgtcctgggc gacgtgcacg accaggacgt
     5881 gtacctcgcg cacctgcgga cctgccggcc ccggccgccg gcgcggatgc tccggtccat
     5941 ccatctccag cgcgaccgcg accggctccg gttcgaggag gactgggccc gcttcgtgaa
     6001 gccgcgctac cagcgcaggc tggatcgcat cctgtccccg cgccgttcgc cctgatggcg
     6061 ggctggccac actttgcaaa gtgtggccag cccctctccg ccgccgaatc atggccgcgg
     6121 ggcgggtttg accttgaatc cggcggcgcg gaggggcaga ttcatgggcg cgccatgagc
     6181 agggcccgcg atacagcctc ggccgcgccg ggcgtggagg cctcggccgg caagtacccc
     6241 ggcaagggct ttcacatcgg cgaatacgtg atcacggatt ttctcggcca cggctcgatg
     6301 gcctccgtgt atctggcggt ggacggcacc ggccacgagg tggccctcaa ggttttccag
     6361 gagggccccg gcgtctcggc gaccatgctc gagcggttcc gccgggaggc ggaggcctcc
     6421 aagaagctgc gccggcatcc caacatcatg ccgatctacg gcaccgggcg cgacggcccc
     6481 taccactaca tcgccatgga gtccatccgg aacagccgca cgctggaggc cgcgctggaa
     6541 tccacgccga tgagcctcct gcgcgtcgtc gagatcgcga tccgcatcgc ccgcgccctc
     6601 cagtacgccc acacgcacaa catcgtgcac cgcgacgtca agccgacgaa catcatgatc
     6661 gacgagttcg aggagccgct cctctccgat ttcggcgtcg cggagctgat ggactggccg
     6721 tcctgcacgc tcacgggcgc gctgacgggg acgccgctct acatgtcgcc cgagcaggcc
     6781 cgcggcgagc gggtcgggcc ggccagcgat atctactcgc tgggcgtcgt gctgtacgag
     6841 gccgtgacgg gcgtgatgcc ctacgccatc cagcacgggt cgcccgtgaa ggacgtgctg
     6901 gaggccgtga agaacgagtt gccgcggcgg ccgcgcctgt tccgcaagga gatctcgccc
     6961 gacctcgagg cggtcctgtt caaggcgctg gagaaggagc cgcgggaccg ctacgtggac
     7021 gccgaggcct tcgcccgcga tctcgagcgg gtccaggcgg gccgtcgcgt cagcgcccac
     7081 cggttttcga tcctcgccta cctgcggcac ctcgcacggc gccaccagca ggggttgctc
     7141 atcttcctgg ccatggccgc gctcctgtcg ggagccggct tgctcttccg gcagcggctg
     7201 ctgcgcacgc gcttcgagga ggtcgtgcgc cgcgcccagt tgaagaacgc cctctacatg
     7261 ctggccgaag ccgagaacgg cgccggcttc gagcgggaga cccctcgggc gtggaacgat
     7321 atccggctgg cgcgcaaggc catgatcggc agcgattggt ccgccgcgcg ggacggtttc
     7381 cgggcggcgg cggacatcag ccgctccgtg ggcgacaccc ggaccgcggc catcgccgaa
     7441 ctggaccagg cccggtgcga aatcatgctg caggaccggg cggccgccca ggccctgtac
     7501 caccgcatcc tgaccaaccc cgacgcctcg ccggcgatcg cgaacatggc ccagatggaa
     7561 agcatcgaac tggccctgct cgcgggcgac cggccccggg cgttcgaggt gctgatgctg
     7621 cgcaccccgc ccgccgaagg gccgatccgg accgcgatca aggggctggc cggcgacctg
     7681 tccagccggg ccgtcgcgga gtcggtggag cgcatgcagc acccgttcca gaacgacgcg
     7741 tacgtggccg cggccatccg ggcctacctg gacggcgaaa tccgtcttgc ggtcgggtat
     7801 atgaggcgcg ccatcgagct atcgtcgccg ccctcggaat ggccggcgcc cttcgcgcgc
     7861 caactcttca acgaggtgga ccgctgacca tggccccgca tcctccagac cccggccgcg
     7921 cacggacgca ccggcgccgc ggcttcacgc tgatcgagct cctcgtggtc gtgctgatcc
     7981 tggccatcgg catcgtctgg accgtccacc acgtgcggca gcagcgactc acgcgccggt
     8041 gcgcggagca gctgtggcac atctacacgg ccctggaaat gtacgaaatc gaccggggca
     8101 cgctgccccg gctggccttt ttcccaaacg atccgaagca ggacgccgac agcctcttca
     8161 ccgtgatcca gccctacacc gcgtcggagg ggctgacggt atgcccggcc ctgcccccgg
     8221 cccagcgggc cctcggcctg acctacgtct ggaacgtgga gaacaacggg cgcaagctgc
     8281 atcctccggg cgtcgccaga tggatgctcg tggagatcag cgcgctgtcg gattccgtgc
     8341 cccctccgca cgaggggcgc taccacatcc tgtactcgga cggccaggtg gagctgtccg
     8401 aaaatccccc gcccgacctg cggcggctgt gaccttcccc gctactggat cgtgctcttg
     8461 gtggccttga ccagcgcctt ccagagctgc tcgggggttt ccgcctcgaa cagccggtcg
     8521 cgcgcgtcct cttcctggaa cgtctgggcc aggccggaca gcagcttgag gtagaaggcg
     8581 ctcgcggccg tggggatgac catgaagaac acgatcctcg tgagcgcccg gcctggcccg
     8641 ccgaaacgga tgcccttgcg gtgcaggccc agcgcgagcg tcaggccccc gccctccacg
     8701 ccgcggacgt ggggaaaggc cagcccatgc tctacggccg tgctgatgat ggcctccctt
     8761 ttcagcgcct cctcctgcag ccggcccgcg tcgtccacga agccttccgc ctccatcttc
     8821 tggcacatct ccgcgatcac ctcgtcgcgc tcctgcgaga ccagctgggg gatcatcagg
     8881 tccacggacg agaagcggtc gatcccgacc ttggcgggct cgctgcgccg tttccggcgc
     8941 ccgatatgtc ggggtaacaa ctcggggtag tacagcatgc gcgcgcagtt cggacaatga
     9001 tgcagcgaat cggcggcatg aacggcgtgc accaggctca ccggcagcga catgccgcac
     9061 gccgagcaca ccccgttcgc cacgggcacg atggccagca ggcccttctt ctcgatcttc
     9121 tggtagcggg cggcgatctc ggggggaagc tgttcgacca gggtctgaat ggaggcatcc
     9181 agttgggaga ggcgggcgcc ctgcatggag gcctcctgtt gggcgcgggc cacgcgcagc
     9241 tcctgcaact ggatcaagtg attgatgacg cggtgcatgc ttcctttctt atgcgcggca
     9301 gcgataacaa cggaatcgct catacacgcc cttcgcccgc cttccgggga caggccgagt
     9361 gacggtttct tctaccaaat gcaaccccgc cctgtaaaga cattccggcg aggagcgtgg
     9421 aagccggatg ggcgcgaatg aacaggcgcc cgggggcggc cgcgcggcag aatggttgcg
     9481 tgttttatga accgaaatag gccggggcgt tgccgggttt ccacttgata ttgcagccga
     9541 ggctcggttt ctggtcggcg gacgccgggc ggcccgcgag cacggcgtcc agggccgcgc
     9601 gcaggtcgcg gccggtcacc ggccggccgt tcccgggccg gctgtcgtcc agttggccgc
     9661 ggtaggccag ccggcggccg tcatcgaaga ggaagaagtc gggcgtgcac gcggccgtgt
     9721 aggccttcgc cacggcctgg ctttcgtcgt aacaataggg aaaggcgaac ccgagttccc
     9781 ccgccatctc cttgagctgc gccggcgcat cgtcggggtg ccggtccgcg tcattcgagc
     9841 tgatggccac catcccgacg tcgcgccccg cgtagtcgcg cccgatccgg gcgatctcgc
     9901 gctccacgtg ccggacgtac gggcaatggc ggcagatgaa catcaccagc agggcgcggc
     9961 ggccggcgaa cgacgcgagg gataccggct ttccgctgac ggtgtccgtc aggctgaaat
    10021 ccggcgcggg ggtgcccagc ggcaacatcg tggacggcgt ggcgaccatg gcggctcctt
    10081 tttcaggaat aaaacccatt tcccggaaga atcaaggccc gctcttctcc cttcaataat
    10141 cccgccccca cttgagccgg agcccttcgc gcaactgcgg gctgctgatg gtctgcaggc
    10201 gcagcgagcg ggacagctcc agttccatcg tgacggcatc ggcctggccc gtcaggctct
    10261 tttccccctc gacgtagacg cggtccccca cgtacttccc gacggcgatc gaggtcaggg
    10321 cgggatcctc ggcgtcctgc cggatgtcca actggtccac gcgcaggagg ttctggccct
    10381 ggccgaggac gtccagcgtg gcgccgccgc cgtgcaggag gttcaggcca taggccaggc
    10441 gcagggcctg cagggccgtg atccggtccg cctcccggtt gaagagcagc tgcgccatga
    10501 tttcgtcctc cggctgcggc gggtcggagg tcagggtcca ggccggggcg ctcaaggagc
    10561 cggccagatc gagccgcgcg gtcagcctcc cggtttccac ggtcgcgcgg atgtccagct
    10621 gcggatcggg cggcgagcgg ccgtccaggt tgatcgcagc gctctccagg ttgaagcgtc
    10681 ggcccaggaa tacgaaccgc ccgcgcagca cgctgaagga gccggccagc gccggttcgg
    10741 ccgccgttcc ccggatgcgc agctggcctt tccattcgga ttccaggccg cgtccccgga
    10801 tgtacacgcg ggccggggcg cggatgtcca ggtccagcga caacgccggc gtcaagctgc
    10861 gggcggccgc cgcctccccc gcccgctccg gaccgttgat ctccgccacg tccagttgcg
    10921 ggatggacgc cgccggccgg tccacggatt cgtagtcgag ctcgcgcacg tcgatgcggc
    10981 cggacagccg gttgccggcc agcgagccgt ccaactgcac ctcgccgtcg gcatccagcc
    11041 ggaagtggtc ctcgcgcgcc agcgcggtgt cttcaaagcg gatggccacc tggtacggaa
    11101 aatggcgggc gggcgcataa tccagccggc cggtcagcgc gatcttgccc tggccgccat
    11161 ccgaggctgt ggcccgggtg atggtcaggc tcgacaggtc ggagcgggcc tcgatgtcca
    11221 cgtcccggag cacgacgccc aggtccaggt tttcatagta accgttctgc agcgccagcg
    11281 cgccctcgat atccggggcc tccaggctcc ccccgaaggt caggccggcg tgcagctttc
    11341 cgcgaatcac gcggtccgca agataagacg ccggcacgag gcgggccagg tccacgtcgg
    11401 cgtccattcg cccatccagc ggcgccgccg gccgcacctc cagagaccag gggcgcaggg
    11461 accaccggaa cgggatgccc acggagcccg aaccctgcac gggacccgaa accgcccagt
    11521 tcgcgcgcag cacaccgtcc gcgaggcaga cacgcagttc ggcatccgcc ggtttaagcg
    11581 ggatcagcag cgcctccccg ggccgaaccg cggacagatt caactgcaat tcagccgccg
    11641 gatcatccac ctggccctgc agttggatcg cgccctccgc ccgcccctcc atcggcgggc
    11701 cacccagatc ggcgattaca cccaggggaa cgccttcgag ccgggccatg gcccggatca
    11761 tccctcccgt gatcgaaccg gccgtccgga agcgaccggc gccgacatgt cccttcaggg
    11821 gctgccaggc gatccagccg tcgccccact ccactcgagc cggctcatcc aggacgaagt
    11881 cgccgggccc gcaccggccg tacgcctcgc gcacggtcag aacggtgccg ggctccgagg
    11941 catcccagcg cccggccgcg gccagttcga aaggccgggc caaccggccg tcggcccgca
    12001 ccgaccagtc gcacgcggac cgggaaccgt ccacgcgaac ggccaggttc gaaatcgagg
    12061 ctcgctttgc gccgacgtcc cgggccacca cctccacgcg gccggtcggg tcgggaccca
    12121 gcgggcacat gtcggcctcc atgcgcagtt cttccgccga agcccacggg gtcgtcacgt
    12181 tacggacccg gccgctggcc cggacgcgct gctcgccgcc cgacgggtcc aggtccacgt
    12241 ccaaccgggc ttccccgccg acgggccagc cgagacgcgc cgccgcgtcg gagagataag
    12301 gcagttcggc ggacaggcgg cccttcgcca gccgggcggc cggatcgaac tccagtccgc
    12361 cggtaacgga cagggcgccg cagcgcgcgt cgagatcccg tatttcgacg cggcgatccg
    12421 cgtaacggac cgcggcccgc gccacgacca cgtcttcccc gcgctgaacg cgcgccgcca
    12481 tttccccctc cggctgctcc agcaggcggc gcagcgcgcc gtccagttcc acgcgctcca
    12541 cgtcccgccg caacagtccg tcgttctcca actccgcgtg aaacgacacg gccagcggcg
    12601 ggcgcgcatc cagcgtgaag gagccgccca gccggccggc ccccgctgcg tcggcctcga
    12661 gggtcccctg gcacgattcg agaggccccc tcgcccccac ccgcgccctg agcggtccgg
    12721 tcggtttcgc cggcagatgc cgggccagcg ggccgccgac cgcatcgagg aggtccacgc
    12781 ccaggcgcca ccccttcgaa cgggaccacg caagggtcgc gtgcgccgcg cccggcaggg
    12841 catcggttcg ctcgccttga atggctccgg cgaagcccgc gacggggtcc cacgaggccc
    12901 gtccgaggac gcgcaatcga aggcgcgctc ccgcgacggg ctcttccagc gccaggcctt
    12961 ccacccggag atgatccacg gccatcggcc aacgaggggg cccggtcttt cgcgttgcgg
    13021 acgtcggctc ggccgccggg agttccgggc ggcggaggac gtgcaccgtg cgcgccgata
    13081 gttcgcgcgc gaccagccgt cccgcgcaga gttctcccgc ggagaggcgc aggcgcaccc
    13141 cggcggcctg cagccactcg cccccggcat ccgacaggcg gatctcgtcc aggcggagtt
    13201 cgaacggcag ccggccatcg agccggccca ggctcaccct ccacccgggc gcgctccacc
    13261 ggcgggccag gacggcggcc aaccggtcct tcgcccaggg ggtctgcaga agcgccaggc
    13321 cgcccgcggc aaccaggagc agaccgccca gcgcgcggag cagccccttc cgcatgccgc
    13381 gggccacccg ccgatcagaa agcctggccg atgctgacat agaattggta atcctcatcc
    13441 acgtccgggc gctggtccaa cgggacggcg acgtccacgc gaaccaggcc gaaggacgtg
    13501 tagtagcgcc agccgacgcc ggcgccccac aggtagttct cgtcgaaatc cggcacgttg
    13561 gactcgaagg cggagccgcc gtccgcgaac aggacgaggc ccatgctgcg cgtcgcgcgc
    13621 cagcgcatct ccaccgagga ttccagcagc gatcgtccgc cgagcgggtc gtcgccctcg
    13681 tcgagcggcc cgacctcctg gtaggcatag cctcggatgg acccgccgcc cccggcgtaa
    13741 aagcgctcgt cggcggggat atccccgcgc gacgggccca gcaggctgcc cgcggcgaac
    13801 cgccaggcca gcacgagcaa aggctgccgg gccaggcgcc agtagccggc cgccgagacc
    13861 tgcgcgcgga agaaggccat ttcgccgttg agcaggtcgt acgtcggcgc ccccaggaag
    13921 gagagccgcg tgccccgcgt cggatccagc aggtcgttgg aggtgtcgcg gatcaggcgc
    13981 gcgggcagcg cccacaggaa atagtcctcc cgttcctcct gttcgacctc ggaataccgg
    14041 agggacaacc cgccctgcgc gcggagcttc ggcgacagct cccgttccag cccggcggcc
    14101 gcccgcaccc actggcttgt ataggcctcc gcggattcgc tctgcgcctt caggtccagc
    14161 agcaggtcct ggtcgggcct cccgaactgg ggccgacgga atgaggcctc ggcgaacagg
    14221 agggattcag acacgcccag ctgcaactcg agctgctcgg cgccgccgaa gaggttgcga
    14281 tgcagccacg acgcgcgggc gtcggcgccc ccgtcggtcg cgtagccgac acccagttcg
    14341 acgcggcggc gcttgcgctc cttcaggata agcgcgaccg ggatctcgtc gccgtcggcc
    14401 gccgcgcgcc cggcgtcgat ctccaccgcg gaaagcgggc cgctcagcgc caggcggtcg
    14461 cgcagccggt ccacccgccg gatgtcgtac caatcccctt cgcgccaggg aatcagcccg
    14521 cggacgaagg actcgcggag acggttcagg ccacggatcc ggacggggcc gaagcggacc
    14581 cgcgggcccg gttcgacccg gaagcggaca tccgcgacgc gcccggcgtg gtccacggtc
    14641 acggccgggc ccgccagccg cgcggccggg tagccgcccg cctggaacgc gcggaggacc
    14701 tgcgcctcgg cgtcgaggac ctcctgcgca aggaggggcc ggcgggccac gaggcccaac
    14761 cgcgcgggcg acgggaccgc gacggattcg ggcggcgcgc cggtgacggc ctcaacggac
    14821 acggaacgga tgagatagac agggcccggc cggatccgga aggtgatccg gggtttttgg
    14881 cgatgccggg aatacgcggg ctccaccgtc gcggcatagt agcctcgggc ccggagggcc
    14941 gcctgcagcg ggacgacatc cttctcggcc cggcggagca ggaggcgctg ggtcggcgga
    15001 ggcttgtgac ggagcagcca ggtttccgtg accgacagga gttcctttcg caaggcgcgg
    15061 ccgggcgcgc cctcaatgcg gacgtcgcag ggcggaacgg tttcggcgcc gagaacggtc
    15121 cgcgccgcca gcagcaggat cgccgttcgc caacagatac gcccgggcca tgctggacgg
    15181 ccaaccttca tgcgatccgc cgaacgctca tggccgcgcg gggcctgaag cgcggggaag
    15241 acacgctacg gggcggcggg cgcttccatg ccggagcgga agcgctccgc gagcgccggg
    15301 ttgagcttcc gccccttctc gaacgcccgt tccgcatcct gggccatgcc ggcgccggca
    15361 taggcccggc ccaactgcag ccagaacatg ggatcgcgcg gccggcggcg gcatgcctgc
    15421 tcgagggcgg tcgcggcgag atcgaatttc ccgagctgca tccggaccat gcccaggctg
    15481 taccaggcgc tgaccatggc gggggagccc cgggtcagca ggtcatagac gagttcggcc
    15541 tcggggaaac ggtccagggc caggtagaag gtcccggcgc cgatcagccg cggcgagacc
    15601 acgggacgcg cgcagcaggc catgcgccgc tggtactccc ggcgctcctg ctccaggacg
    15661 agcaggccgg ccttctggat ctcctcgttg gacagccagg cctggttcgc cgcggtgggc
    15721 cggatgagga gggcggacgt gccgtccaga taggcgagcg cccagcgccg gctctccagc
    15781 agcatgcgga gcagcgggcc cgcgcccagc gcgcagccgt tcagcagcac cgattccggc
    15841 tcccacttct tctccagcgc gtcccaggcc tcggcctctc cgttcaggct gcgggcgacg
    15901 gcctcaaaaa actcgggggc gtgcaggtcc gcgcgctggt ccacgaagac ggggcgcccc
    15961 ggcgcggccc acaggaggaa attgccgtcc tgaaagagat gaacggtctt cgcggggaag
    16021 cccggcttcg ccaggatctc cgcggccttc tcggggtaga gcccgctcgc cacgccgaac
    16081 ccggtcacgg agggcgagcc gacgctctcg tagaaatgat tggagagaac ggccgcggcg
    16141 gaaccggcgg cgaggagcag gaccagcagc gcgccggcgc gggaccacca ggccgcccgg
    16201 gtcccgaggg cgggccgcac ccggtcgccc aggtaggccc ccgccgcgga gccgctcaag
    16261 gccaggaaag gaaaggccag cagcgcgaag aactccacgt gccgcagcga acggacgacg
    16321 aggaaggcgc ccagcacggc caggctggtc atcgcgatcg gcagcttccg cttttcggtg
    16381 atcagcccga tcgccccgat gagcagggcc agcgtcacga tgttcttcac gaaaaaggcg
    16441 cgctggaatt gaagactgaa cggcgagatc cattcctgga cgtacgcgaa caccacgagg
    16501 ctccagtcgc ggaatgtctg ggtgaagacc gccgcgccga agggattgag caggctcaca
    16561 acgagggccg cggggatcat cgccgccgcc cagcccaccg ccttcttgtc ctgcgactcc
    16621 cggttgccgt gccagcgctc gatggcgaac aggaggaaga ggatggggcc cagcacgaag
    16681 ctcccgtgca cgttggccca cagccattgg acgggcaaca aggtcaccgg catccaggcg
    16741 cgcgggctct ccgagagcac acggatgaac agcgccgcga acaggagcga gggcagcacc
    16801 ggggaaacca cgaagcgaac gctcaacagc caggcgcagc ccagcagggc cagggccgtg
    16861 cccaccggcc cggccaggcg ccgcgcgggc cggatcagca ggccgaacgc caggaccacg
    16921 aaggcggcgt gcagcagggt caccagggcg gccccgccga ggttccagag gccgtacagc
    16981 aggcgatcgt agagccagga cgtgttgacc cagggcaggc cggccgcggt aaacgtgaag
    17041 gcgtcggccc gagggatccc ctgctcggca atcgcccgcc cggtggccag gcgaagccag
    17101 atgctgctgt gcccgatggt gcgaatgccc cacaaggcca acacggccag cccgacccag
    17161 gccagccagc cgccgccctg tttctgttcc cccgcctgcg gtgacgtctc gttcatgaat
    17221 tcagccctcc acgcttcttc gaactcggtt cattctgtgg gatgcccggc gcggcggcaa
    17281 gaggtaaaaa gcccggtcac gggcccggcc cgccggccgc ctcggcccgc acctgctcga
    17341 cgtccagcgc gatctgcacg cgcagcgatt ccaggctgtc gaagcggcgc acctcccgca
    17401 gccggcgccc gaaccggatc tccatgtccc gcccgtagag ttcgcccccg ctgaaatcca
    17461 ggagatggac ctcgaccagg ttcaccccgg ccggaaggcc ctcgtgcggc ttgctgtcca
    17521 ggaacgccgc gcccgtccag gcgccgtcct tcatctcggc gcggaccgca tagactccgg
    17581 gcggcggcag cgcctcgtta tgcgggtcca ggttggccgt ggggaagccc agccgcgtgc
    17641 cgacccggcg gccggccatg acggtgccca gcaggctgta cggccggccc agcatgaccg
    17701 cggcatcgtc caggcgcccc tcctgcaccg cgcggcggat ccgcgtgctg gagatggcga
    17761 ggccgtccca caggacgggc tccacgatgg tcgcctgcaa gccctgcgcg gcgacccact
    17821 tccgcagcag gtccgcgttg ccccgcccgc gatggccgaa ggtccagttg gaccccacca
    17881 ccagctggcg cgcgcccggc caggccccgg tgagccgcgc caggaacggc cccggatcga
    17941 gggccgccag ttcgggcgtg aacgccagca cgaggcagcc ctcgatcccc agctcgtgca
    18001 ggagccggat cttgtggggc gtggaggtca gcgagagggg cgcttcccgc gggcgcagga
    18061 tcttcatggg gtgcggatcg aacgtcagca cccaggccgt tccgcccatg gccgccgcct
    18121 gatccagcgc ggctgcgaat gggccgccac cggcaaggtc gagtgaccgc cgatcgtgaa
    18181 gcacgagcct gagcccatcc tggtcagcgt gccctgtacg gtcgtctggc gcggccgccg
    18241 gatcctgcag
//
LOCUS       DAOOTK010000056        17417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2235_length_17417_cov_4.874093, whole genome shotgun sequence.
ACCESSION   DAOOTK010000056 DAOOTK010000000
VERSION     DAOOTK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_17417_cov_4.874093"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK634_07885"
     CDS             <1..637
                     /locus_tag="PK634_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOW97693.1"
                     /translation="LCRRVSYRKFQPIYNEGEGSRKIHAVLKGEVVLHKLGDGTREPV
                     RLAVVRAGEMFGFGEAMLETYYTSAGAATACILLEIGRDDFVRRFLAVPAFRVQVVRA
                     LSKIARYLVDKAARGSGLNDLALYLQALSGECGENRMGKIFIRKKLHQPEVASLLNLS
                     REHVTRLFARLKAEGVVDFNRGYPIIDRAWLDRVVRDKDLAASIQYRDAPF"
     gene            695..>938
                     /locus_tag="PK634_07890"
     CDS             695..>938
                     /locus_tag="PK634_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97694.1"
                     /translation="MKHFLPGLPWLLPVWLLFANRAAGTSLTITITNLPPSGSTAEGR
                     SVALAGTLNGWNNNSTLATVTGGRLVYTFADVGSLPA"
     assembly_gap    939..948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <949..3377
                     /locus_tag="PK634_07895"
     CDS             <949..3377
                     /locus_tag="PK634_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOW97695.1"
                     /translation="WSDKPDGANAAFQFVAPGSWSERAVKADFNSNDGNFRVALVEGA
                     ANTVEIDAGPVPTLVDQAGAVRVNGVAEREVPALDPACFAFPGGRCKALVMSYDDGHV
                     QDRGLVGIFNAHGIRGTFHLNSGSLNSDTFVTTAEIPSLYAGHEVSSHTVHHPYLDGL
                     DDGGVQWEVGTDRATLGAAAGYTIRSLSYPFGAFNNRVISLLAGLGVKSARTTLDSYT
                     LEYFPPNPLKWHPTCHHSAAQGLAEAFAARAEERMALLFIWGHSYELDYNLSNNSWAY
                     MDALCATLGDRGDVWYATMGEVEEYLSAARALEWPAPNRVRNPSGSVTVWTRLAGRLS
                     KVRPGANLTYPAGSVGVSPGDPAENTTITLRYAPGTNAFREAAALQIHIGRDGWQEVR
                     DLAMTREGDLAWTCAIEVPDGARRLDFAFTDGQGLWDDNSGADWSLTIRSAFPVALAP
                     VQCAPGSPAIAERSATGQNSAGESLDLDPSGGALTTSAAAGFGNFGSVYVNADATNLY
                     LGATGCDLSGDNNGMILFLSVGSLADGVANLWNLRGAPAGLDRLHNLGFAPAANVAIV
                     LGDEYGDGAFPHFNLGNGYDFGQGVFYLSSGAAMFQPVPGARLSQFDGSGATPTGTSD
                     DDANRLTDRWEVAIPWTSLNAALGAASLGSLHVSGVIASDSVSGNDRYLSARFLGESA
                     AGELDAYGNFGFHFVTLTGRRVGLPGADSDLDGMTDLHETLAGTDAADPASALVMLRP
                     VPRDGACDLQFESVPGRQYRIQYRTDLAPGGVWLDLPGPLTATGGVCGATDPAAGESR
                     VFYRVRLVGP"
     gene            3441..4154
                     /locus_tag="PK634_07900"
     CDS             3441..4154
                     /locus_tag="PK634_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOW97696.1"
                     /translation="MDPATNQDESYREFKRDLWMSALALVSIGIGLYQLGQAGAAPGM
                     TALDYVDLGIVVVFIAEFVLSARRAGNFRAFLARRWWELPSMIPITGGMITGLGGISL
                     VRGVRLLRLVRAIRLLRVVSVGLRLRRVSGFIARVYRRSQAGSLVAVAAVIVLLGAVA
                     AFAAESRHSEHFATFQNSLWWSLNMFTNVAYVDFQPATGLGRLLAGILQVFGIAFIGI
                     FAGSMANAIMQESKPPAAP"
     gene            4248..5192
                     /locus_tag="PK634_07905"
     CDS             4248..5192
                     /locus_tag="PK634_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOW97697.1"
                     /translation="MILSILLFVLGIALLWVGTEMVLRRIPTLAAWLRVSPLVVTILL
                     VAVMTSLPEFCVSFFAALRGQAAAAVGNIVGSNFVTLTFVAGLCALWRPIVVGPAIRE
                     RESSWMILSAALMLVLAMDGRISRLDGLILLAAYVPFFHGTLVEARRERQHAAPAGVA
                     RPWIDIVFFLGGVGLIILGSSWIVEHGTVMARRLGMSDLLIGVTFYAFGTSLPELAIA
                     LGAVIHRQADVTLGEIYASNIFTALAAAGALCLALPLSVEPIIVQRDLPLTIVAGVLL
                     QMFVTTGRRFVRSEAVVMIALYALFLAAQFMGFNISLP"
     gene            5361..6137
                     /locus_tag="PK634_07910"
     CDS             5361..6137
                     /locus_tag="PK634_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOW97698.1"
                     /translation="MLGALAGDIIGSVYEWNNIKTKQFPLFSPQCFFTDDSVLTVALA
                     DAILTGTPYAQNLKKFFLLYPKRGYGGGFARWASSEDSKPYHSFGNGAAMRISPAGWA
                     YDTLEEVLDKAREFTAVTHNHPEGIKGGQATAAAIFLGRAGRTKDDIRAYVEDNFGYD
                     LSRHVDEIRPAYAFNETCQETVPQAIRAFLDSTDFEDAIRTAVSLGGDTDTLACITGG
                     IAEAFYGGVPPAIRNKAYAILDPRLGDITRAFVARHCAKS"
     gene            complement(6169..8247)
                     /locus_tag="PK634_07915"
     CDS             complement(6169..8247)
                     /locus_tag="PK634_07915"
                     /inference="COORDINATES: protein motif:HMM:NF025622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HOW97699.1"
                     /translation="MPQDATQPTGETQDRDPAGSPDAGALGGRIRRAWERIPARFKKA
                     FALAFGVNVLVFAYLLLQHPLGNHDISRLPWVEYRNQVSVGRWFCFFLYLLVGHAQLP
                     VVNQLWAIVFHVSSGMAAAVYWRRAGGAYPLTVAALMASLVPFVLAYFYYSYQSLCFG
                     AAQLLAIGGLIAAARATRSGVALGALLIMCALASYQPALNVAAVALMLRALLALSDSR
                     ARGETPAMGRFLRTTLGPGALALIAGGVLYRLSLALCRWLGLIEWNAYQLREASLGHL
                     PSRLARSLEAAFAHLVIPQPYMLWPVKLILLALLVMAAWVALRRLGTGPGTRAQRIRY
                     TAWGLLLLLGAIWATKSIFAVSPIRFYYAFRLAGGLTLLYLFGALLAMEAPSPRGRSL
                     AAALAGAALLLFAHQDLVHSGLMVRSNQHDLQMANRILDRIETLPGLDADETYNLIVI
                     GDLPRYAMQRYTERTRRFEEPADYMDEHSMVPPWEPELVFAMLGSSVSLQTPGRDRIQ
                     DVRRQQGYRYALQHAAWPAKDSVALLDGDLVVVVLEGDVAELAKGYAQLARRLRSVDD
                     SDSARVAAVHADIEERLGQLYTRLEYRAGRKLGWIERQAAATQAGWEEAAADGAQPDP
                     GIERLLQQVDEGIRSARARLDSTMAHTNASTAVRLETLQELCNEVLPPLQRALRHARE
                     SNRPALHDGG"
     gene            8400..9479
                     /locus_tag="PK634_07920"
     CDS             8400..9479
                     /locus_tag="PK634_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97700.1"
                     /translation="MRLVPGAREASPPLSNNAERGAELACAGVAGWMAPTPERPEERT
                     GDALLQILRQRGLADTVILGGAARDRLLAVSSRCDVDLQMRIRLTDAERSRIGSIRRL
                     ARFRWPWGGLLYSLAMRCAMRGIQARHEPDWAPLLWDGVLENPEGLFPGTASFRGVRV
                     ERAKRVLVTAEGEIIPENRVPSFELVALSPEGEVFAYDAQTLEDIEKRRVRIAGCGAL
                     SIRTVMRMVSIRRQFAGTSYDEGSWREMEAYVGWLRRGRGAARFHLQSRHARRVILAR
                     LGETIQRAGDQWPLAVEDLDRLGLLPLIESACPEAGSLIRYRRALDGGAAVSGDEAAF
                     CEPLLSSFPRQVFPLDAPPRMGARP"
     gene            9476..10711
                     /locus_tag="PK634_07925"
     CDS             9476..10711
                     /locus_tag="PK634_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW97701.1"
                     /translation="MSAHSAGRNGSSFASSISLLSIMLFTVLGTTILRPVIDLNLNRA
                     GLTKTYIGANGVMWELGCFLAALLLVRGLRRWGQRTLLAVCLSLLPLLILATPPLLPT
                     PFLFPLRFALGFLTTLVCLACITGSVVASGKENHGTALCVITASATGAKALGSAMVRQ
                     VGIDGYGPFLLAGMSVGLGLLLLASLRNGAPKGGLGPQRSPLACAKVSPFALGTLVIL
                     AFCAGACRAAFFTFLPIYATGIGFRPEDGALLLAVAFAGAVILSVPIGRLGDRFGHGR
                     MLVLVMLVVIAASIPLVAPVTGLPWLMVMVFVIGGGFSTTRDLGQAVWSCRHPEPENV
                     IAHLSFWAGLGAVVGVLGTGVFMDLWGPLAFGAAVIGGGVLIMIMAASTDPRALKVDE
                     DTERDMDPLVGANKILFDD"
     gene            10725..12569
                     /locus_tag="PK634_07930"
     CDS             10725..12569
                     /locus_tag="PK634_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97702.1"
                     /translation="MKIGLFQGRDWKKRGMAAAVLLLVLASAAVLVWNRREPPPPPPL
                     PLLPDEASLAESDVGLLATDPATGSGPFPYLVPMVQAIDYRLRDIELAGRNRGYRFRH
                     VFQAGCGSGFLRENTVGNDFDYMITVHLGELATDLADPVQAADDLLTRIESYLGLFYR
                     VVHDQAGPDLAMHQWPDMRGDRLRNRSRVQQHLAESLTAFRDGRPRCFLLDGEQGRRV
                     PCCVAAGELSLPTDLLASLLSNRVQYGPRMYAGIRGVGVLFRFYCDLVYPGPDGPPVT
                     RRNVPVNPLHSSGRVLDMRNGFIGMAPAGAESAAFLRAEIASDPARWVRYRVDVGADL
                     LSQVTRYLDEQKPMKALKRLHQSYDFLEPMLDPDAFAGLRPFLRRHLQDPDVLLCEDI
                     RELSSQAAVAVDEPWLRRLYAGSGDLPRTLRQILLNLARFEKRGPPALAQEAAALKAA
                     LEPIVAEGSRSYGSRERMQIEAALEAIEQQAARWSAALLEPAAGEIAQWRDRIRQELA
                     ACGVRPFRAYGLPTNEVVGILSADMAGVVTMDALNQLAAQTNVPPFQYCSLEPDQIPP
                     DRQGETDYFPCYLWLRPAPGPAEEERFRQALDRIEADMVRSGLRVQPP"
     gene            12566..13429
                     /locus_tag="PK634_07935"
     CDS             12566..13429
                     /locus_tag="PK634_07935"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOW97703.1"
                     /translation="MTTPAMNDGVASARPWKKRLVVAAWAVGVAAILGAGLYVGVHRP
                     ASDARRRSTWKAPSGAQIERDEIYMLLAYAVAARGWQVEGPAKRGFNVGAVLVDSSGS
                     NALFWARNCNVLLQNATQHAELRLMLGYLDAARVYNLMGYTVYVTLEPCAQCAGMMTM
                     TGVRRVVYGQRDPEYGRAFERLQLDTRGNPGRLRGGWKGSYGPYPRAVRVSRSATNYG
                     ARLEEAYKRSKQDLPDFLCSESAKAVFDEARRALDEYRVRYEENQSILSSARKMLEQV
                     PAEFVPLGLRM"
     gene            13467..15773
                     /locus_tag="PK634_07940"
     CDS             13467..15773
                     /locus_tag="PK634_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97704.1"
                     /translation="MKRITYTIGLISALQVGLEILFIKLARYDFGTFSIAVVGVAMLG
                     IGAAGLLVHVWSARRAAVLERSLLALPVLILLSGIVFFSSPQSLGRLGAVFTRLGLEA
                     AAVFLVMGCSAIPVFALMEEQSQRVSLLYGASLLGAALGAVASFVLSHVWGDIVAYAA
                     MSALLAFLWLPPSCAGRVAGLIRGVGVLTAVAGFVLYPFLERRAHPDNLYSRSNSFTR
                     IDVIRDSDTLLHIKTGGVNAGTSVLLDGYAPLARRFRRDASAIPFYLGLKRALILGSG
                     GGKNVCQALAFGVDQVTAVEINGLITDFMEGALPEHVNPYRDPKTRLIVGEGRAAVST
                     LRKAGTRFDLVYVTLATVFGSSGHVFTHTYLMTRDAVRDYLGLVDEGGIVAAYHLGGE
                     RLRAKIARAFFDALLAVEPGAAPEQVAVFAQDAESPMSRFVVMARKGRPFTAAEAEQI
                     KEQVREVEQMQSAREIEAGRSLILLTDNNPFLHNDTEVPLTSRRFFVWNHRMLNPLLG
                     ATAVLCLALCLRGAWRKGKGAGLPFADGLSCLAAFSGIGFAYTLQQTLVLQKLEFFLE
                     HPVTNTFVVLPLSLVGTGLGSMATGVFVRRLSRTGLLLLRLILPSVVVGLAAVPSAWL
                     LGFAWPLPVRIVASAFLVLPCFFLMGTYFPVFFARTARLEPALLPWCWAVNATAAVVG
                     SLFFIVVGMQVGFRALALVPAAVYLLVSLWDWRREQGGAGEGSGTALTSSGAGAFRML
                     VLAGLGVLAILFLLWLVVTRLNILSMHP"
     gene            15787..16023
                     /locus_tag="PK634_07945"
     CDS             15787..16023
                     /locus_tag="PK634_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97705.1"
                     /translation="MNGNARTIGRAIWALLVLAFFYTAFFMLAGGEPGRGSCVTRPDP
                     QGGLVFVVLPLLQLIGVFIGLAMAALLESSGRAS"
     gene            complement(16088..16164)
                     /locus_tag="PK634_07950"
     tRNA            complement(16088..16164)
                     /locus_tag="PK634_07950"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16128..16130),aa:Met,seq:cat)
     gene            complement(16222..17283)
                     /locus_tag="PK634_07955"
     CDS             complement(16222..17283)
                     /locus_tag="PK634_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97706.1"
                     /translation="MSIIREPAAGGGSYVFEDDFEDGDLAGWTEGTTGHWTNTTSGAI
                     TGTRSLKHNLSSVETTSCIYAQPVYSLAGTQTLWRFNLKGGNWDPSADNRFWVYLAAN
                     EADLGGATVDGYAVGVNLAGSSDTVTLWRVANGAAAATIIQSALDWSAYTTGGIQVSR
                     SAAGVWELEVDSNGGFDNLVSAGTGSDTTYTDTSYFGLFFDFTATRAGYLWMDDVRIW
                     QTNSIGEDADSDGMADWWEAMYFTNGVSALPGDDADGDGSPNYNEFIAYTIPTNRGSV
                     FELTSPSNAAGGVFYFTWPSATDRLYTVRVTTNLLNSFSDWSNNIAATPPANIFRDAV
                     HTNDIRLFYNVNVDYPGAP"
BASE COUNT         2445 a         5940 c         6079 g         2943 t
ORIGIN      
        1 gttgtgccgc cgcgtttcgt atcgcaagtt tcagccgatc tacaacgagg gcgaggggtc
       61 ccggaaaatc cacgcggtct tgaagggcga ggtggtcctc cacaagctgg gtgacggcac
      121 gcgcgagccg gtgcggctgg ccgtagtccg ggcgggggag atgttcggct tcggcgaggc
      181 gatgctcgag acctactaca ccagcgccgg cgccgcgacg gcctgcattc tcctcgaaat
      241 cggccgggat gatttcgtcc gccggttcct ggccgtgccc gccttccggg tacaggtcgt
      301 gcgggccctc tcgaagatcg cgcgctacct cgtggacaag gccgcgcgcg gcagcgggct
      361 caacgatctg gccctgtacc ttcaggcctt gagcggcgag tgcggagaaa acaggatggg
      421 caaaatattc atccgcaaaa agttgcatca gcctgaagtc gcgtcgctcc tgaacttgag
      481 ccgcgagcat gtcacgcgcc tgttcgcgcg gctcaaggcc gaaggggtcg tggacttcaa
      541 ccgcggctac ccgatcattg accgcgcctg gctggaccgg gtggtgcggg acaaggacct
      601 ggccgccagc atccagtatc gcgacgcgcc gttctgacct attcggtgat gcacgtcact
      661 gacgcatttc ccgtgcgggc gtagggtggc gcgcatgaaa cacttcctgc ccggcctgcc
      721 ctggctgctc ccggtctggc tgctgttcgc aaatcgcgcc gcggggacca gcctgaccat
      781 caccatcacc aacctgccgc cctccggttc gacggccgag ggccggtcgg tggcgctcgc
      841 ggggacgctc aacggctgga acaacaactc gaccctggcg acggtgacgg ggggccgcct
      901 ggtgtacacg ttcgcggacg tcggatcgct gccggcgcnn nnnnnnnnac tggtcggaca
      961 agccggacgg ggcgaacgcg gcgttccagt tcgtggcgcc gggttcgtgg tcggagcgcg
     1021 ccgtgaaggc ggacttcaat tccaacgacg gcaacttccg cgtcgcgctg gtcgagggcg
     1081 ccgcgaacac ggtcgagatc gacgcggggc ccgtgccgac gctggtggac caggccggcg
     1141 ccgtgcgcgt gaacggggtg gcggagcgcg aggtcccggc cctggacccg gcctgcttcg
     1201 cgtttcccgg tggccggtgc aaggccctgg tgatgagcta cgacgacggc cacgtgcagg
     1261 accgcggcct ggtcgggatc ttcaacgcgc acgggatccg cggcacgttt cacctcaatt
     1321 ccggctcgct gaattccgac acgttcgtca cgaccgccga aatcccgtcg ctgtacgccg
     1381 ggcacgaggt ctcctcgcat accgtccacc atccgtacct cgacggcctc gacgacggcg
     1441 gcgtgcagtg ggaggtcggg acggaccgcg ccacgctggg ggcggccgcc gggtacacga
     1501 tccgcagcct ctcgtacccc ttcggcgcgt tcaacaaccg cgtgatctcc ctgctggccg
     1561 gcctgggcgt gaagagcgcg cggacgacgc tggactcgta caccctcgag tacttccctc
     1621 cgaacccgct caagtggcac ccgacctgcc atcattcggc cgcgcagggc ctggccgagg
     1681 cgttcgcggc gcgcgcggag gaacggatgg ccctgctttt catctggggc cacagctacg
     1741 agctggacta caacctctcc aacaacagct gggcgtacat ggacgcgctc tgcgccacgc
     1801 tgggcgaccg cggcgacgtc tggtacgcga cgatgggcga ggtggaggaa tacctctccg
     1861 ccgcgcgggc gctcgagtgg ccggcgccca accgcgtccg gaatccgtcg ggttccgtca
     1921 cggtctggac ccggctcgcc gggcggctgt ccaaggtccg gccgggggcg aacctgacat
     1981 acccggccgg ctcggtcggc gtctcgcccg gcgatcccgc ggagaacacg acgatcacgc
     2041 tccgctatgc ccccggtacc aacgccttcc gagaagcggc cgcgctccag attcacatcg
     2101 gtcgggacgg ctggcaggaa gtgcgcgacc tggccatgac gcgggagggc gatttggcct
     2161 ggacctgcgc catcgaggtt ccagacggcg cgcgccggct ggacttcgcc ttcaccgacg
     2221 ggcaggggtt gtgggacgac aacagcggcg ctgactggtc gctgacgatc cgatccgcgt
     2281 ttcccgtggc cctcgcgccc gtccagtgcg cgcccggctc gcccgcgatc gcggaacgtt
     2341 ccgccacggg ccagaacagc gccggggaat ccctcgacct cgatccctcg ggcggcgcgc
     2401 tcacaacctc ggcggccgcc ggcttcggga atttcggcag cgtgtacgtc aatgccgacg
     2461 cgacgaacct ctacctcggt gcgacgggat gcgacctgtc cggggacaac aacggcatga
     2521 tcctgttcct gtcggtcgga tcgctggcgg acggcgtggc gaacctgtgg aacctgcgcg
     2581 gcgcgccggc cgggctggac cggctccaca acctggggtt tgcccccgcg gcaaacgtgg
     2641 ccatcgtgct gggcgacgag tacggcgacg gcgcctttcc gcatttcaac ctcgggaacg
     2701 gctacgattt cgggcaaggc gtgttctacc tgtcctccgg cgcggcgatg ttccagcccg
     2761 tgcccggcgc ccggttgtcg cagttcgacg gcagcggcgc gacgccgacg gggacgtccg
     2821 atgacgacgc caaccggctg accgatcgct gggaagtcgc gatcccgtgg accagcctga
     2881 acgccgcgtt gggcgccgcc tcgctgggct cgctgcacgt gtcgggcgtg atcgccagcg
     2941 acagcgtctc ggggaacgac cgctatctct ccgcccgttt tctcggcgaa tccgcggccg
     3001 gcgagctgga cgcgtacggc aatttcgggt tccatttcgt cacgctgacc ggccgccgcg
     3061 tcgggttgcc cggcgcggat tcggacctgg acggcatgac cgacctgcac gagaccctgg
     3121 ccggcaccga cgcggccgac cccgccagcg cgctggtcat gctccggccc gtcccgcggg
     3181 acggcgcgtg cgacctgcaa ttcgagtccg tgcccggccg gcagtaccgg atccagtacc
     3241 ggaccgacct cgccccgggc ggcgtatggc tggatctgcc cggcccgctg acggcgacgg
     3301 gcggcgtgtg cggcgcgacc gacccggcgg ccggcgagtc ccgcgtgttc taccgggtgc
     3361 gcctggtcgg gccgtaaagc gctggtcctt ttccggcttc aaccgccgcc gcgaatccgg
     3421 tataagcgcg gcaaccactc atggaccccg caacaaacca ggacgaatcc taccgggaat
     3481 tcaagcgcga cctgtggatg tcggcgctcg cgctggtgag catcggcatc ggcctctacc
     3541 agctggggca ggccggggcc gcgcccggca tgacggccct cgactacgtg gacctcggga
     3601 tcgtcgtggt cttcatcgcg gagttcgtgc tgtccgcccg gcgggccggc aatttccggg
     3661 ccttcctggc ccggcgctgg tgggaattgc cgagcatgat cccgatcacc ggcgggatga
     3721 tcacgggcct gggcggcatc tcgctggtcc gaggcgtccg tctgctgcgc ctggtccgcg
     3781 cgatccggct gctgcgggtc gtgagcgtgg ggctccggct gcgccgggtg tccggcttca
     3841 tcgcccgcgt gtaccgccgg tcgcaggccg gctcgctcgt ggccgtcgcg gccgtgatcg
     3901 tcctgctcgg cgccgtcgcg gcctttgccg cggagtcgcg ccatagcgag catttcgcga
     3961 ccttccagaa cagcctctgg tggtcgctga acatgttcac caacgtcgcc tacgtggatt
     4021 tccagcccgc gacgggcctc gggcgcctgc tcgccggcat cctccaggtg ttcggcatcg
     4081 cgttcatcgg catcttcgcc ggcagcatgg ccaacgccat catgcaggaa tccaagccgc
     4141 ccgccgcccc gtgagcggac cggcggctcg atcaacgccg attcctcttc cggcgggccg
     4201 cttttaattg cttcccgtcg ggtggccgtt ggtctagaaa ggcgggcatg atcctgtcca
     4261 tccttctctt tgtgctgggc atcgcgctgc tgtgggtcgg cacggagatg gtgctgcggc
     4321 gcatcccgac gctggcggcg tggctgcgcg tctcgccgct ggtggtgacc atcctgctgg
     4381 tcgccgtgat gacctcgctg cccgagttct gcgtgtcgtt cttcgccgcc ctgcgcgggc
     4441 aggccgccgc cgcggtgggc aacatcgtcg gctcgaattt cgtcaccctg acgttcgtcg
     4501 ccggcctctg cgccctgtgg cgcccgatcg tcgtcggccc ggcgatccgc gagcgggagt
     4561 cgagctggat gatcctttcc gcggccctga tgctcgtgct ggcgatggac gggcggatca
     4621 gccggctgga cgggctgatc ctgctggcgg cctacgtgcc ttttttccac ggaacgctgg
     4681 tggaggcgcg gcgcgagcgc cagcacgcag cgccggccgg ggtcgcgcgg ccatggatcg
     4741 acatcgtctt tttcctgggc ggggtggggc tgatcatcct cggctcgagc tggatcgtgg
     4801 agcacggcac cgtcatggcg cggcggctgg gcatgagcga cctgctgatc ggcgtgacgt
     4861 tctacgcgtt cgggacctcg ctgcccgagc tggcgatcgc gctgggcgcg gtgatccacc
     4921 ggcaggccga cgtaacgctg ggcgagatct acgcctcgaa catcttcacc gcgctcgccg
     4981 cggcgggcgc cctgtgcctg gcgctgccgc tgtccgtcga gccgatcatc gtgcagcgcg
     5041 atctgccgct gacgatcgtc gcgggcgtgc tcctgcagat gttcgtcacc accggccgcc
     5101 ggttcgtgcg gtcggaggcg gtggtgatga tcgcgctcta cgcgctgttc ctcgcggccc
     5161 agttcatggg cttcaacatc tcgctgccgt gaggccgtac agcccgcgga ccgtgccggg
     5221 aaaggactct ctccctcctt tttccgtctc ccctccgtgg tttcctcccc attcccccgc
     5281 gtgatccggc cgggcgcgcg gtgaagcgtc tttccaagcg caggggagag tcgtatacat
     5341 aacaaaaagg agggacgatc atgctcggcg cgctggcggg ggacatcatc ggctcggtct
     5401 acgagtggaa caacatcaag accaagcagt ttccgttgtt ctcgccgcag tgcttcttca
     5461 ccgacgacag cgtgctgacc gtggcgctgg cggacgcgat cctcaccggc acgccctacg
     5521 cacagaacct gaagaaattc ttcctgctct acccgaagcg cgggtacggc ggcgggttcg
     5581 cgcgctgggc gagcagcgag gattccaagc cgtaccacag cttcggcaac ggggccgcga
     5641 tgcggatcag ccccgcgggc tgggcctatg acacgctgga ggaggtgctc gacaaggccc
     5701 gcgagttcac ggcggtgacg cacaaccacc cggagggcat caagggcggg caggccacgg
     5761 ccgccgcgat cttcctcggg cgcgcgggaa ggacgaagga cgacatccgg gcgtacgtgg
     5821 aggataactt cggctacgac ttgagccggc acgtggacga gatccggccg gcctacgcgt
     5881 tcaacgagac ctgccaggag accgtcccgc aggcgatccg ggctttcctt gattcgaccg
     5941 acttcgagga cgcgatccgc accgccgtct cgctgggcgg cgacacggac acgctggcct
     6001 gcatcacggg cggcatcgcc gaggccttct acggcggcgt cccgccggcc atccggaaca
     6061 aggcctacgc catcctcgat ccccgcctcg gcgacatcac ccgcgccttc gtggcccggc
     6121 attgcgccaa gtcctgaacc gccgggcggc gagcgtccgc cctgcggttc agccgccgtc
     6181 gtgaagggcc gggcggttgc tttcccgcgc atgacggagg gcgcgctgca agggcggcag
     6241 gacttcgttg cagagttcct gcagcgtctc gagccggacg gccgtgctcg cgttggtatg
     6301 ggccatggtc gaatcgagcc gggcccgggc ggaccggatg ccctcgtcga cttgctgcag
     6361 caggcgctcg attcccggat ccggctgcgc cccgtcggcc gcggcttcct cccacccggc
     6421 ctgcgtggcc gccgcctgcc gttcgatcca gcccagtttt cgcccggccc ggtattccag
     6481 gcgcgtgtac aactgcccga gccgctcctc gatgtccgcg tgcacggccg cgacgcgggc
     6541 ggaatccgaa tcgtccacgg aacgcagccg gcgcgcgagt tgggcgtagc ccttggccag
     6601 ttcggcgacg tctccctcca ggacgaccac gaccaggtcg ccgtccagca gggccaccga
     6661 gtccttcgcg ggccaggccg cgtgctgcag ggcgtaccgg taaccctgct gccgccggac
     6721 gtcctggatc cggtccctcc cgggggtttg cagggacacc gacgacccga gcatggcgaa
     6781 aacgagttcg ggctcccacg gcgggaccat agagtgctcg tccatgtagt cggccggctc
     6841 ttcgaaccgc cgcgtgcgct cggtgtaccg ctgcatcgcg tagcgcggca agtcgccgat
     6901 cacgatcagg ttgtaggtct catcggcgtc caggccgggc agcgtctcga tccggtcgag
     6961 gatccggttg gccatctgca ggtcgtgctg gttgctgcgc accatcaggc ccgagtgcac
     7021 gaggtcctgg tgcgcgaaga ggagcagggc ggcgcccgcg agcgcggcgg cgaggctgcg
     7081 cccccgcgga ctcggcgcct ccatcgccag cagcgcgccg aacagataca ggagcgtgag
     7141 cccgcccgcg agacggaagg cataatagaa gcggatcggg ctcaccgcga agatactctt
     7201 cgtggcccag atcgcgccga ggagcaggag caggccccag gccgtgtacc ggatgcgctg
     7261 cgcccgcgtc ccgggccccg tcccgagccg gcggagcgcg acccacgcgg ccatgacgag
     7321 cagcgcgagc aggatcaatt tgaccggcca gagcatgtac ggctggggaa tgaccaggtg
     7381 agcgaacgcc gcctccaggc tgcgcgccag gcgcgagggc aggtgcccca gggacgcttc
     7441 gcggagctgg taggcgttcc actcgatcag cccgagccaa cggcagaggg ccaggctcaa
     7501 ccggtacagg acgccgccgg cgatgagggc gagggcgccg gggccgaggg ttgtccggag
     7561 gaatcggccc atggcgggcg tctcaccccg ggcgcggctg tccgacagcg ccagcagcgc
     7621 gcgcagcatc aaggcgaccg cggcgacgtt cagggccggc tggtaggagg cgagggcgca
     7681 catgatcagc agcgctccca gcgccacgcc gcttcgggtg gcccgcgccg ccgcgatcag
     7741 tcccccgatg gccagcaact gcgccgcccc gaagcagagc gactggtagc tgtaatagaa
     7801 gtacgcaaga acaaacggca cgagggaggc catcagcgcc gcgaccgtca gcgggtacgc
     7861 gccgcccgcc cgccgccagt agacggcggc cgccatgccg ctcgaaacgt ggaacacgat
     7921 ggcccagagc tggttgacga ccggcagctg ggcgtgcccc acgagcaggt agaggaagaa
     7981 gcagaaccag cgccccaccg aaacctggtt gcggtactcg acccagggca gccgggagat
     8041 gtcgtggttg ccgaggggat gctgaaggag gaggtacgcg aaaacgagga cgttgacacc
     8101 gaacgccagg gcgaacgctt tcttgaagcg ggcggggatg cgctcccacg cgcgccgaat
     8161 gcgcccgccc agggccccgg cgtccggcga gcccgccgga tcacggtcct gcgtttcccc
     8221 ggttggctgt gtcgcgtcct gcggcatgac aaatggaagc tttctcctcc gcttgtcgaa
     8281 acaatgctgc cacgggggaa ggattgtcaa gcgccggagg tcgcgcgcct gttgaccggc
     8341 cggcgcgagt gctgtatcct gcgcctatgg gtggtccaac gaggtgcgac cgagtcgacg
     8401 tgcggttggt cccgggtgcc cgggaggcat ccccccctct ctcgaacaac gcggagcgcg
     8461 gcgccgaact cgcgtgcgcc ggcgtggccg gctggatggc cccgacgccg gagcggccgg
     8521 aggaaaggac gggggacgcg ctcctccaga tcctgcggca gcgcgggctg gcggacacgg
     8581 tcatcctcgg cggcgcggcc cgggaccgcc tcctcgccgt cagcagccgc tgcgacgtgg
     8641 acctgcagat gcgcatccgg ctgaccgatg ccgagcgctc gcggatcggg agtatccgcc
     8701 gcctggcccg cttccgctgg ccgtggggcg ggctgctcta ttccctggcg atgcggtgcg
     8761 ccatgcgcgg aatccaggcg cgccatgagc cggactgggc cccgctgctg tgggacggcg
     8821 tgctggagaa tccggaaggg cttttccctg ggacggccag cttccgcggc gtgcgcgtgg
     8881 agcgggccaa gcgggtcctg gtcacggccg agggggagat cattcccgag aatcgcgtcc
     8941 cgtccttcga actcgtggcc ctctcgccgg agggggaggt cttcgcctat gacgcgcaga
     9001 ccctggaaga catcgaaaag cggcgggtcc gcatcgcggg ctgcggcgcg ctgtcgattc
     9061 gcaccgtgat gcggatggtt tcgatccggc ggcaattcgc ggggacctcc tacgacgagg
     9121 gatcctggcg cgagatggag gcctatgtcg gctggcttcg gcgcggccgg ggcgcggcgc
     9181 gctttcacct gcagtcgcgg cacgcgcggc gggtcatcct ggcgcggctg ggcgagacca
     9241 tccagcgggc cggcgaccag tggccgctgg ccgtggagga cctggaccgg ctcgggctcc
     9301 tgcccctgat cgaatcggcc tgccccgagg cgggcagcct gatccgttac cggcgcgcgc
     9361 tggacggcgg cgccgccgtg tcgggcgatg aggccgcgtt ttgcgaaccg ctgctgtcga
     9421 gcttcccgcg ccaggtcttc ccgctggatg cgccgccgcg gatgggagcg cgtccatgag
     9481 cgcgcactcc gcaggccgga atggctcgag cttcgcctcc tccatttccc tgctctcgat
     9541 catgctcttc acggtgctcg ggacgaccat cctgcgcccg gtgatcgacc tgaacctgaa
     9601 ccgggccggg ttgaccaaga cctatatcgg cgcgaacggc gtgatgtggg aactgggctg
     9661 tttcctggcg gccctcctgc tggtgcgcgg cctccgccgg tggggacagc gcaccctgct
     9721 ggccgtatgc ctgagcctcc tgccgctcct gatcctggcc acgccgcccc tgcttccgac
     9781 gccgttcctg ttcccgctcc gcttcgccct cgggttcttg accaccctcg tgtgcctggc
     9841 gtgcatcacc ggctcggtgg tggccagcgg gaaggagaac cacgggaccg cgctgtgcgt
     9901 catcacggcg tcggccaccg gggccaaggc cctgggctcc gccatggtcc ggcaggtcgg
     9961 catcgacggc tacgggccct tcctcctcgc cggcatgagc gtcggcctcg gcttgctgct
    10021 cctggcgagt ctccggaacg gcgcaccgaa gggcggcctg ggcccgcagc ggtctccgct
    10081 cgcgtgcgcg aaggtctctc ctttcgccct gggcacgctg gtcatcctgg cgttctgcgc
    10141 gggggcctgc cgcgcggcct tcttcacgtt cctccccatc tatgcgaccg gcatcgggtt
    10201 ccgtcccgag gacggcgccc tgctgctcgc cgtcgccttc gccggggccg tgatcttgag
    10261 cgtgccgatc gggcggctcg gcgaccggtt cgggcacggc cgcatgctgg tgctggtgat
    10321 gctggtcgtc atcgccgcct caatcccgct ggtggccccc gtcaccgggc tgccgtggct
    10381 catggtcatg gtctttgtga tcggcggcgg attctcgacg acgcgggacc ttgggcaggc
    10441 ggtgtggagt tgccggcacc ccgagccgga aaacgtcatc gcgcaccttt ccttctgggc
    10501 cgggctcggc gccgtagtcg gcgtcctggg cacgggggtg ttcatggacc tgtggggccc
    10561 gctggccttc ggggccgccg tcatcggggg cggggtgctg atcatgatca tggccgcctc
    10621 cacggatccc cgcgcgctca aggttgatga agacaccgag cgcgacatgg atcccctggt
    10681 gggcgcaaac aagatcctgt tcgacgattg acgcgagacg cacgatgaag atcggactgt
    10741 tccagggccg ggattggaaa aaacgcggga tggcggccgc cgtccttctg ctggtcctcg
    10801 cctccgccgc cgtgctcgta tggaaccggc gggagccgcc cccgccgccg ccgctcccgc
    10861 tgctgccgga cgaagccagc ctggccgagt cggatgtggg gttgctggcg acggatccgg
    10921 ccaccgggag cggccccttt ccctacctgg tcccgatggt ccaggccatc gactaccggc
    10981 tgcgcgacat cgaactggcc ggccggaacc gaggctaccg gttccggcac gtgttccagg
    11041 ccgggtgcgg gtcgggcttc ctgcgcgaga acacggtggg caacgatttc gactacatga
    11101 tcacggttca tctcggcgaa ctggcaaccg acctcgccga tccggtccag gccgccgatg
    11161 acctgctaac ccgcatcgag agctacctgg ggctgttcta ccgcgtggtc cacgaccagg
    11221 cgggccccga cctggcgatg caccagtggc cggacatgcg cggagaccgg ctgcgaaacc
    11281 ggtcccgcgt gcagcaacac ctggccgagt cgctcacggc tttccgggac ggccgccccc
    11341 ggtgcttcct cctcgacggc gagcagggcc gccgggtccc ctgctgcgtg gcggccggcg
    11401 agttgagcct cccgaccgac ctcctggcca gcctgctgtc caaccgggtc cagtacggcc
    11461 cccggatgta cgcgggcatc cggggcgtgg gcgtgctctt ccgcttctac tgcgacctgg
    11521 tgtatcccgg gccggacggg ccgccggtca cgcggcgtaa cgtgcccgtc aacccgctgc
    11581 attcctccgg gcgggtgctg gacatgcgta acgggttcat cggcatggcc cccgccggcg
    11641 cggagtccgc ggcgttcctg cgcgcggaga tcgcctccga cccggcgcgc tgggtgcgct
    11701 accgcgtgga cgtgggcgcg gacctgttga gccaggtgac gcgctacctg gacgaacaga
    11761 aacccatgaa ggccctcaag cgcctgcacc agtcctacga tttcctggag ccgatgctcg
    11821 acccggacgc ctttgcgggc ctgcggccgt ttctgcgccg gcaccttcag gatccggacg
    11881 tgctgctgtg cgaggatatc cgtgaattgt cctcgcaggc cgcggtggct gtcgacgagc
    11941 cctggctgcg ccggctctac gccggctcgg gcgacctgcc ccggaccctg cggcagattc
    12001 tcctgaacct ggcccggttc gaaaagcgcg ggccgccggc gctggcacag gaggccgccg
    12061 cgctcaaggc cgccctcgaa cccatcgtcg ccgaaggcag ccgctcgtac ggcagtcgcg
    12121 agcgtatgca gatcgaggcg gccctcgaag ccatcgagca gcaggcggcc cgctggtcgg
    12181 ccgctctgct ggagccggcc gcgggagaaa tcgcgcaatg gcgggatcgc atccggcagg
    12241 aactggccgc ctgcggagtg cggcccttcc gggcctacgg cctgccgacg aacgaggtgg
    12301 tcggtatcct gtctgccgac atggcgggcg tcgtgacaat ggatgccctg aaccaactgg
    12361 ccgcgcagac aaacgttccg cccttccagt actgctcgct cgagccggac cagatcccgc
    12421 ccgatcgcca gggggaaacg gactactttc cctgctacct gtggctgcgg ccggcgccgg
    12481 gcccggcgga ggaggagcgg ttccggcagg cgctggatcg catcgaggcg gacatggtcc
    12541 ggtccgggct ccgggtgcaa ccgccatgac gacccctgcg atgaacgacg gcgttgcttc
    12601 tgcccggccc tggaagaagc ggctggtcgt ggcggcgtgg gcggtcggcg tggccgcgat
    12661 tctcggcgcg ggtctgtacg ttggcgtcca ccgaccggca tcggacgcgc gccggcggtc
    12721 gacctggaag gctccctccg gggcgcaaat cgagcgcgac gagatctaca tgctgctggc
    12781 gtacgccgtc gcggcaaggg gctggcaggt ggaggggccc gccaagcgcg ggttcaacgt
    12841 gggggccgtg ctggtcgact cgtcgggctc gaacgccctg ttctgggcgc ggaactgcaa
    12901 cgtccttctg cagaacgcca cgcagcacgc ggagctgcgg cttatgctgg gctacctgga
    12961 tgccgcccga gtctacaacc tcatggggta caccgtgtac gtcacgctcg agccgtgcgc
    13021 ccagtgcgcg ggcatgatga cgatgaccgg cgtccgccgc gtggtctacg ggcagcgcga
    13081 ccccgagtac gggcgggcgt tcgagcgcct gcaactcgac acgcgcggga atcccggccg
    13141 cctgcgcggt gggtggaagg gcagctacgg gccgtacccg cgggccgtcc gggtgtcccg
    13201 gagcgcgacg aactatggag cgcgcctgga ggaggcgtat aagcgctcga agcaggacct
    13261 gcccgatttc ctgtgcagcg aatcggcgaa ggccgtcttc gacgaggcgc gccgggcgct
    13321 cgacgagtac cgcgtgcgct acgaggaaaa ccagtccatc ttgtcgtcgg cgcggaagat
    13381 gctcgaacag gtgccggccg aattcgtgcc gctcggcctc cgcatgtgag gccgggcggc
    13441 ggaacgccgg ttttcgcgga aaaagcatga aaagaataac gtataccatc ggcctgatat
    13501 cggccctcca ggtggggctg gagatcctgt tcatcaagct ggcgcgctac gacttcggga
    13561 cgttctcgat cgcggtcgtc ggcgtcgcca tgctgggcat cggcgcggcc ggcctgctgg
    13621 tgcacgtctg gtccgcccgg cgcgcggccg tgctggagcg gtcgctcctg gcgctgcccg
    13681 tcctgatcct cctctcgggc atcgtgttct tctcgtcgcc ccagtccctg ggccgcctgg
    13741 gcgcggtctt cacgcggctg ggcctggagg cggccgcggt cttcctcgtc atgggctgct
    13801 cggcgatccc ggtcttcgcg ctcatggagg agcagtccca gcgggtttcc cttctgtacg
    13861 gcgccagcct gctcggggcg gcgctgggcg ccgtggccag cttcgtgttg agccacgtct
    13921 ggggcgatat cgtcgcctat gccgcgatgt ccgccctgct ggcgttcctc tggctgcctc
    13981 cttcttgcgc ggggcgcgtc gccgggctga tccgcggggt aggggtcctg acggcggtcg
    14041 ccggcttcgt gctctatcct ttcctggaac ggcgggcgca tcccgacaac ctgtattccc
    14101 gcagcaactc cttcacccgg atcgacgtca tccgcgacag cgacaccctc ctgcacatca
    14161 agaccggcgg cgtcaacgcg ggcaccagcg tgctgctcga cggctatgcc ccgctggccc
    14221 ggcgcttccg ccgcgatgcc agcgccatcc cgttctacct gggcctcaag cgggcgctga
    14281 tcctcgggag cggcggcggc aagaacgtct gccaggcgct ggccttcggc gtcgaccagg
    14341 tgaccgccgt cgagatcaac ggcctcatca cggacttcat ggagggcgcg ctgccggagc
    14401 acgtcaatcc gtaccgcgat ccgaaaacgc ggctgatcgt cggggagggg cgggccgcgg
    14461 tctccacgct gcggaaggcc gggacccgct tcgacctggt atacgtcacc ttggccaccg
    14521 tgttcggatc ctcggggcac gtgttcaccc acacctacct gatgacgcgg gacgcggtgc
    14581 gcgactacct gggcctggtg gacgagggcg ggatcgtggc ggcgtaccac ctcgggggcg
    14641 agcgcctgcg cgcgaagatc gcccgggcgt ttttcgacgc gctgctcgcc gtcgagccgg
    14701 gggccgcgcc ggagcaggtg gcggtgttcg cgcaggatgc cgaatcgccc atgtcgcgct
    14761 tcgtcgtcat ggcccgcaag ggacgccctt tcaccgcggc cgaggcggag caaatcaagg
    14821 agcaggtgcg cgaggtcgag cagatgcaga gcgcccggga gatcgaggcg ggccgttccc
    14881 tgatcctgct gaccgacaac aacccgttcc tgcacaacga cacggaggtg ccgctcacgt
    14941 cgcggcggtt cttcgtgtgg aaccaccgca tgctgaatcc gctgctgggt gcgacggccg
    15001 tgctgtgcct ggcgctgtgc ctccggggcg cctggcgaaa ggggaagggc gccggcctgc
    15061 cgtttgccga cggtctgtcc tgcctggccg cgttcagcgg tatcgggttt gcctacacgc
    15121 tgcagcagac cctcgtcctg cagaagctgg agtttttcct tgagcatccc gtgacgaaca
    15181 ccttcgtggt attgccgctc tcccttgtgg ggaccgggtt gggctcgatg gcgaccggcg
    15241 tgttcgtccg ccggctctcc cggaccgggc tgttgctctt gaggctgatc ctgccgtccg
    15301 tggtcgtggg cctggccgcg gtgccctccg cgtggctgct gggtttcgcc tggcccctgc
    15361 cggtccgcat cgtggcctcg gctttcctcg tgctgccttg cttcttcctg atgggcacgt
    15421 actttcccgt gttcttcgcc cggaccgcgc ggctcgaacc ggccctgctg ccctggtgct
    15481 gggccgtcaa tgccacggcc gccgtcgtcg gctcgctgtt cttcattgtc gtcggcatgc
    15541 aggtcgggtt ccgcgccctg gccctcgtgc cggcggccgt gtacctgctg gtcagcctct
    15601 gggactggcg ccgggagcaa ggcggcgccg gggagggatc gggtaccgcg ctcacgtcgt
    15661 caggcgcggg tgcttttcgc atgctggtgc tggccgggct cggcgtgctg gccatcctct
    15721 ttctgctctg gttggtggtg acccggttga atatcctgtc gatgcatccc tgaccagggc
    15781 cttgccatga acgggaacgc ccgcacgatc ggtagagcca tttgggcgct gctggtcctg
    15841 gccttttttt acaccgcgtt cttcatgctc gcgggcggcg aacccgggag aggatcatgt
    15901 gtaactcgac cggatcccca aggcgggctc gtcttcgtag tcctgcccct gttgcagttg
    15961 atcggggtgt tcatcgggct cgcgatggcc gccctcctcg aatcgtccgg ccgcgcttcg
    16021 taacgccacg gctttcagcc ggtcagcgaa aaagggcctg aaattcatca tttcaggccc
    16081 gcaaagttgg tagcgggggt tggatttgaa ccaacgacct tcaggttatg agcctgacga
    16141 gctaccgggc tgctccaccc cgcagtcagg gcgaggaatg tacaggaccc ccgccaggat
    16201 gcaagctatt ttcaggaacc gtcagggcgc gccggggtag tcgacgttga cgttgtagaa
    16261 caggcggatg tcgttcgtgt gaacggcgtc ccggaagatg ttggccgggg gggtggccgc
    16321 gatgttgttg gaccagtcgc tgaaggagtt cagcaggttg gtcgtgacgc gcacggtata
    16381 cagccggtcc gtcgcgctgg gccatgtgaa gtagaatacg ccgcccgccg cgttggaggg
    16441 gcttgttagc tcgaagaccg agccacggtt ggtcgggatg gtgtaggcaa tgaactcgtt
    16501 gtagttgggc gaaccgtcac cgtcggcgtc gtcaccgggc agggcggaga cgccgttggt
    16561 gaagtacatg gcctcccacc agtccgccat gccgtccgag tcggcgtcct cgccgatgct
    16621 gttggtctgc cagatgcgga cgtcgtccat ccagagatac cccgcgcgcg tcgcggtaaa
    16681 gtcgaagaac agcccgaagt aggaggtgtc cgtgtaggtc gtgtccgacc ccgttccggc
    16741 ggagacgagg ttgtcgaacc cgccgttgga gtccacctcg agttcccaca cgccggccgc
    16801 ggaccgagag acctggatgc ctcccgttgt gtatgcgctc caatccagcg cggactggat
    16861 gatcgtagcc gccgccgcgc cgttggccac gcgccagagc gtcacagtgt ccgaggaacc
    16921 ggccaggttc acgcctacgg catagccgtc cacagtcgcc cctcccaggt cagcctcgtt
    16981 ggctgcgagg tagacccaga accggttgtc ggcggacgga tcccagtttc cgcccttgag
    17041 gttgaagcgc cacagggtct gcgtcccggc cagcgaatag accggttggg cgtagatgca
    17101 gctcgtggtt tccacgctgg agagattgtg tttcagcgag cgcgtccccg tgatggcgcc
    17161 ggaagtcgtg ttggtccagt gccccgttgt cccctcggtc cagccggcca ggtcgccgtc
    17221 ctcgaagtcg tcctcgaaga cgtagctccc gccgcccgca gccggctcgc ggatgatgga
    17281 caccgaatcg ctgctcgcgt cgccgaggta gttcgtgccc cggacggtga tgacgttcgt
    17341 gccgacgttg agcccgatgc ttccgctgga ccaggtcgcg gcggcggtct tcctgtcacc
    17401 gagccggttc cgtcacc
//
LOCUS       DAOOTK010000057        17319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2257_length_17319_cov_6.404367, whole genome shotgun sequence.
ACCESSION   DAOOTK010000057 DAOOTK010000000
VERSION     DAOOTK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17319
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_17319_cov_6.404367"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5089)
                     /locus_tag="PK634_07960"
     CDS             complement(<1..5089)
                     /locus_tag="PK634_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOW97707.1"
                     /translation="MKSGCRWFITAALFGLASGASAQGIWISNGLSPSAKGYLAVKVS
                     FGGYCNDGYVGADSGTVGFLYEFLTYFSVNDGGAMLPSATPVDLGSNRVQCSDTLDGE
                     YGKIQYTVLTWIPPGETALYQRWTLTRAGGLGDLRCVPYVDEDVGDTFDDVFIPLGTY
                     ASGSLELHTVDQASRVGLSQGVLKTDERNVTWAGWHADWYDALQEHIRYNGATNFPAA
                     GYINTSHLPLIGGSSPPEYGPSDVTSALAYDVNPASTQAVFTAILGTVPHFDYFTLTV
                     QSQDPSSGAAVTVSPADNEGNGDGSTPLARTYVAGKAVTLTAAAAAGTNVFSRWLLDG
                     ADQGTNRSLVVTLSTNRTATAVYGGGGAATNLLPDLSVRSFRSDKTLVNAGEPFWVEA
                     SVTNYGPGHAGACHARLYLTPSNDFTLQDDFLIGRIPIPALNANTDTWVRWTFTAPHF
                     PFPSNAVWTIVHLDTDSEVTETNENNLFKSVNPFYSILAPTGSVRVLSVFSSNPDVDV
                     SIGVVPPDKNGDANGLTTVTRGYATNTTVSLTAPTFADGNPFDHWLLNGADAGNNAML
                     TVPLTSNQTARAVYGLSAGLSVASQDPDGGVAVEVAPADRNGNADGITPFNRAYTSNL
                     TAVLTAPEMAEGKPFSHWLLDGTNKGASRIVNAALATGSIHSATAVYSVTGALPDLIW
                     SGHAWRTNVLREGDAFWINCIITNIGQGAAGPSHATFHLSPGNDWDLTDDFCAGEQSV
                     GAMGPGGAQSIRWEFAMPDRGSGTYTQWILVDVDCRGEVDESNEASPYKGGSTLLVSD
                     GTATGRTLTVLSSSPDAGIAIDVAPADLACESTGTTAFTRAYTNGAVVTLTAPSNAGA
                     QVFHRWLLDESAADTNRSVAVTLTTNRTARAVYGPAVDDAVLWGSATNEFGRAVRADG
                     AGNVYTVGYGYGPLDGQTNAGDSDIFLRKFGPDGSGVWTRVFGSAGHDEAEELCLDGD
                     ANLYVVGYTEGAFHGESYTGARDAFLAKYSPAGDHQWTHFFGSSFSDYGNGVHVGSDG
                     FVYMAGTTYGAFEGQTNTGGADFFAAKFSADGTLAWTRIWGSVSNDHARGIGPGPSGS
                     AMFGGFTAGALAGQTNTGGNDFLAARLSTATGTSLWTRIWGSASDENAEDIASDTRGN
                     TYLFGYSYGAFHGQTNAGRWDFALTKLNAGGTAAWTRIWGSATNDYGLGVCLDDAGNA
                     YAVGYTTGGLDGQTNSGWYDAAVSAFTPSGTRIWMRLFGGALGDYGMEAAADGARNLY
                     VVGTTYNALFGQFLNGGADLMLVKFATRGNTYALAVQSANPDAGVPVTVTPDERSGIV
                     TGTTPFAQAYLENTRVTLAAPGTMGATRFDRWEVDGVPQGSNRTVAVSLSADREAVAY
                     YEGSSAGGDLVFGSGDDEYAGGVCTAGDGSVYVAGYSDGEFGGQTNAGLFDACLRKHS
                     AAGAVQWTRLWGGDSYDYGQAVAAAPDGGAYVAGYTYGAFDGQTNSGSLDLFLSRFDA
                     AGARQWTRVWGSAEWDYGAAVAVAPDSNVYVAGFAFGALDGQTNNGGADFLLSKFNPA
                     GTRLWTRLWGSPDDEMGRGVCVDGGGDIFVTGSSDGHFDGVVNSGRYDLVVSKFSAAG
                     ARQWSRSKASSAAEVGFGIAADTNGNLYVAGSTDGPLGGEPHAGETDLCLVKFDANGT
                     PQWTRVWGSTAADHGSGVAVDEAGRALV"
     gene            5264..6340
                     /locus_tag="PK634_07965"
     CDS             5264..6340
                     /locus_tag="PK634_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97708.1"
                     /translation="MKAVLRGLGVAAGLVLLPLIGVKIRGYPVTRYLEFPPLTRYVEH
                     AGFSWPAFVVLTLLGLALVVPMVRVLVRAWADRPRSKPARPFPAWGWFGVVFTAAAWV
                     LAWNRFPWFAPLQRFTFTPLWLGYIVLVNALAYRRTGACMMTARPRFFLALFPVSAVF
                     WWYFEYLNRFVQNWYYTGVEHISPLQYFIEATLPFSTVLPAVLGTCEFLGSVFGRRPA
                     ASEPSSGRPAPAVLLLAVAALGLAFLAVWPDMLFPLLWISPLLVLSAMRMLSDRPFLD
                     PGPDGSAARRAALFAASALICGFFWEMWNFHSLARWVYAVPFVNRFHLFEMPLLGFAG
                     YLPFGLECALIGDAVGAWIRKNDE"
     gene            6330..7814
                     /locus_tag="PK634_07970"
     CDS             6330..7814
                     /locus_tag="PK634_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97709.1"
                     /translation="MTNDLTGRLACAARRVNLGAAAAISLYFFILPGLSVLRDLADPA
                     LRSDAIPRAAWRLFEDLTPRYERWARGRIGSGKAESLALHEIAGTEWPLFGSVFYLWS
                     VESLQEDWERRANPAGPAPREFARGAVEAATDLVLDPGHAAWVRLHWGDDYLHRENVF
                     YRMLLISAMTSHRRLTGDARHRPMLREQVETLSAELDRSPHGILNDYPGECYPMDVLM
                     AVAAIRRADAVLETDHSAFAARSLRGFQGARLDPRGLPPYVADPVRGVAFGAARGCGI
                     SYAFMSAPELWPDIARDWYARYEQWFWQEKYGLRGFREFPRDLGGGDWYMDVDAGPVL
                     GGVGVSASAFGVAGSRIMGRFDRAWPLAAQMTVFGWPLPNGTWLTPRILSNAANAPLL
                     GEACILYSLTRRAAPGMEIRTGGSLPPIVVLAWVVYFGLGFLFPAAEVHAVRRWRARG
                     APVRAPALQFAIWLALVLAAAALAVRFGPTAGFAALFPALLFPR"
     gene            7904..8809
                     /locus_tag="PK634_07975"
     CDS             7904..8809
                     /locus_tag="PK634_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97710.1"
                     /translation="MSSLILRGSSIAWKASVLAGATAWAVRGAVADTLFFTNEADWVA
                     ATTNRELFQTTAANVGLADEVDAPAQNNVDVGSNLTFQAVNTGLPWSFRVATLQAGAR
                     FTFSDNEEGAGYPPYLGFEEALSVGDIDDYEDDDFEVVILDGPNLHGVAMDIGENDPL
                     IADGGVYVYDPAAQLIGYSSIVPARSNAFAFLGIVSDAAIGRVVIDEDTGGDDVSVRH
                     FRFARHRTADPAVTAWAPLAGGQARLEWPSASDWRYDVEACADLVGEFAPFATNLPAT
                     PPVNGLTNILGPGRAFFRVRARAEP"
     gene            8887..9453
                     /locus_tag="PK634_07980"
     CDS             8887..9453
                     /locus_tag="PK634_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOW97711.1"
                     /translation="MTAFFEMLTTMQKFYAVCALFGALLFIVRTILMFLGHDDGIDTD
                     HPADMSGDADASFKALSLQGLTAFFMMFGLVALAISKQAGASNLWAVLGGLVAGLFTV
                     WVIGRIFIGMKKLQADGTLRIETAVGQEGEVYLPLAPGKSGQVRVAVQGQLRVFDAVT
                     EGGAELKTGARIRVVRVTAGNVLVVEKI"
     gene            9477..10880
                     /locus_tag="PK634_07985"
     CDS             9477..10880
                     /locus_tag="PK634_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOW97712.1"
                     /translation="MLPIVVLAFVILIVSTIMFLAARYKRCPSDRILVIYGKVADNRS
                     ARCMHGGGTLVWPLIQDYAFLSLTPMTISIPLQNALSQQNIRINVPSTFTVGISVDPN
                     IMLNAAERLLGLESGDIEAMAKEIIFGQLRLTVASLTIEQINQDRESFLESIRKNVEP
                     ELNKIGLYLINVNITDITDESLYIESIGKKAAAEAVNKARVDVAEQDKVGAIGQAEAN
                     REQTVRVAENVAAAEKGKKKAEADQRIYVQGQEAQAVGGENTAKADIADSNSLLAVKQ
                     AAAMQKAEVARREAQVQIQKAQYLAEQERLNAELVVQREIEKRNIEIMAEAEAERIRR
                     EAKGQADGTLLKYEAEAKGVRLVLDSKAAGYTALVKSCNGDAKAAATFLMVEKIEAVV
                     ARQVEAIRNLKIDKITVWDSAGGGEKGSSTANFVSSLIKSVPPLQDVASMAGVELPEY
                     LGRMTEDKAKKKPAGEA"
     gene            11084..15652
                     /locus_tag="PK634_07990"
     CDS             11084..15652
                     /locus_tag="PK634_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW97713.1"
                     /translation="MKTRTSALGAVLFLFMAGTSLAASSLSIFGSDAENRVYHFFIDE
                     SAGETNVITFEFWPNDGTPTVAEIFTTLNHRDQATLSPPDPNTVAQGDASGYWGAYTM
                     ADAGGGKWTLALPIQKCGSYDVRARYKVSGDANWRWYAGRNPVVNISDVATRDMVVYE
                     MQANVVDATGNDYATRSTFASLTNSAKNWNLDYISALGVNTIWLQPFHPIGAKSDCNS
                     GDPGSPYSIKNLFQVAEHLGSDGTRATAMREFTNFVIAADAKGVKVICDVIFNHVATD
                     IEIERDPDNPTNLYANPLAEMRSVKYPWFSRYTGTRSGCPTTKPQSDWANYHYTETAT
                     SASEIGPAPADRNDFVWPDAFDLFWGTYPALGNINDTSDGAWTASADVKKMVEYYSYF
                     IRYWHEKTGGKIGGFRCDFAQGLPRQAWQYLINRAKSLKPELYFVSESLDGGNIAYRA
                     WKGGFDALNENELWAIVEDGDIQSTDLRNVIDQRKTQFGLALILRGTMNHDQGPWIGR
                     KWDGVAMHSVFCSVDGTPQLYEGQELGYDALGQFSRERVEFGRTIPDIRNYHNMNNLW
                     SGRYSGDNESLWHRYKDANLGRSRSLALRVADQYYLDRTGGQGPHPKIFSVLKYTRYG
                     WDPKDQDVVVAFVNLQPGTANSGTFNVNVAPIYLDPTRTYNVKNLASTTPDTYLWASG
                     RSGADIAANGIYVGFSADKGAEGSIAQFLKLEEQGGGPGPSTNLQWIGNTRHWPVNGS
                     ITAADDLWIDIESWPTNTATGGTVVYSTDGVGWNTRELSTNGVAGNNNAWHVNLGQFS
                     AGARIQYAVQIVQSGGTNFWDNSGGTNYLAVVNSGGSGQPLGWIGAVSHWPTNGAITS
                     ADDLWVDIQSWPTSSAISGFVVYSADNGQTWPTMPLSLNATSPSNDFWHANLGRCSSG
                     ASIQYAVSLWDDSGTNQHWANAGGTNYWAFVGGTGSTRSVHWAGNTISRSLRAPCLAA
                     VSLSGDGLKVESSSAIVGTEYRMYRSTNLVSWDLVTNVVAGGSTFSVTNPPSDGPSAF
                     YRAVGQNVPYEVTVFEGDDLIVRAESWPQGRAAGANIVYSSNGQNWYGMSMTWIGTSL
                     NNDLWEANLGFFPKGISVQFAIEIVDDQGFTLWDNNGSQNYSVAIRDPAQPDVAAPVL
                     SYSPSNTTTAAAFLDVTLAAVDDYDAHPLITYTLDGSTPVWDSELYTAPLHITNDLTI
                     KAIAIDASGNTSAVTSVSVKVNDVVELGAFKPYSVNPTLGRAATITIDGSPSDWTTNM
                     LVALDVADDDPRTLGDNWTTHEAPLDFAYLWAAWDDTYLYLAWQYVDLTDIIDGANAG
                     SAAGGKISSNDGILQWIAIDTIQGQGAAKDVWSKNGGQPYWTGTDRPDYQVYMAGSLW
                     QGYISRAVNGVFALDDGGVNYKTAAAAGISYAKGSTLGAGTLWMPSAHHADGRFGALS
                     IATHDTTRDSFYEIRIPLSFLQVTRAQLESNGIGVMMGAGSVSCMDSIPHDETTLDTP
                     GVEVWNSSKEWTDTDRFTTRFARVGHQK"
     gene            complement(15660..15824)
                     /locus_tag="PK634_07995"
     CDS             complement(15660..15824)
                     /locus_tag="PK634_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97714.1"
                     /translation="MHRFESDPRLQPDGGTLMQGGPVIPSPRGLVVAGKWLLRLPAIL
                     ALYFKRKGRL"
     gene            complement(15793..15867)
                     /locus_tag="PK634_08000"
     tRNA            complement(15793..15867)
                     /locus_tag="PK634_08000"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15833..15835),aa:Cys,seq:gca)
     gene            15940..16614
                     /locus_tag="PK634_08005"
     CDS             15940..16614
                     /locus_tag="PK634_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97715.1"
                     /translation="MIRRSYLLRRELACAAVLGGWWASGCQAPPASAVNSEPEQRVAT
                     LRVLGASHELREDQVYPAPETRHVLRWKAGRFEWQITLADRAEPYAAVLLQRPLDLRR
                     YRPKSYLRFRIQPDVAAADLYLGLADRRADGTDPVMVILPLAPYRTWQRNALGYYAIP
                     LKAFGDRGWVLASNRQRAPSLHAGAMDWSRVGEFRIGTQGRPAWRKPFVVSRFEIGVS
                     PRVSEE"
     gene            complement(16624..>17319)
                     /locus_tag="PK634_08010"
     CDS             complement(16624..>17319)
                     /locus_tag="PK634_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOW97716.1"
                     /translation="LKLDTLEPLQKLVRSGRHDFGVAYDGDADRAGFVDEKGNVIPMD
                     MITALIAQTILQHEKGPIFYDLRSSWAVKEVIEENGGTPMMSRVGHAFIKAQMREAKA
                     LFAGELSGHYYFRENGYTESASLAALYIANLVSQSDRPLSELVKPIRRYFASGEINSE
                     VHDVKGVIGRLRAKYAAGRMIELDGLSVEFDDWWFNVRASNTEPLVRLNLEAKTADLM
                     ARHRDEVLAVIRG"
BASE COUNT         2731 a         5921 c         5892 g         2775 t
ORIGIN      
        1 agaccagggc gcgcccggcc tcgtccacgg cgacgccact gccgtggtcg gccgcggtcg
       61 agccccagac gcgcgtccat tgcggggtcc cgtttgcgtc gaatttcacc aggcacaggt
      121 ccgtttcgcc cgcatgcggc tcgccgccga gcggcccgtc cgtcgagccg gcgacataga
      181 ggttgccgtt ggtgtccgcc gcgatgccga acccgacctc cgccgcggac gaagccttgc
      241 ttcgcgacca ttgccgcgcg ccggcggcgc tgaacttgct gacgaccagg tcgtaccgtc
      301 cggagttgac cacgccgtcg aaatggccat cggacgagcc ggtcacgaag atgtcgccgc
      361 cgccgtccac gcagaccccg cgccccatct cgtcgtccgg cgagccccag agccgggtcc
      421 acagccgggt cccggccggg ttgaacttgg agagcaggaa gtccgcgccg ccgttgttgg
      481 tctgcccgtc cagcgcgccg aacgcgaacc cggcgacgta gacgttgctg tccggcgcga
      541 ccgcgacggc ggcgccatag tcccattcgg cggagcccca gacgcgggtc cactgccgcg
      601 cgcccgccgc gtcgaagcgg gaaaggaaga ggtccagcga gccggagttc gtctgcccgt
      661 cgaaggcgcc gtaggtgtac ccggccacgt acgcgccgcc gtcgggcgcg gccgcgacgg
      721 cctgcccgta gtcgtaggaa tcgccgcccc agagccgcgt ccactgcacc gcgccggccg
      781 cggagtgctt ccgcagacag gcgtcgaaga ggccagcgtt ggtctgcccg ccgaactcgc
      841 cgtcgctgta ccccgcgaca tacaccgagc cgtcgccggc cgtgcagacg ccgccggcat
      901 actcgtcgtc gccggacccg aagaccaggt cgccgccggc ggacgagccc tcgtagtacg
      961 cgaccgcctc gcggtcggcg gagagcgaca ccgcgaccgt gcggttcgag ccctgcggca
     1021 cgccgtccac ctcccagcgg tcgaatcgcg tcgcgcccat ggtcccgggc gcggccagcg
     1081 tgacgcgggt gttctccagg taggcctgcg cgaacggcgt ggttccggtc acgatcccgg
     1141 atcgctcgtc gggtgtgacg gtcaccggca cgccggcgtc cgggttcgcg gactgcacgg
     1201 cgagcgcata cgtattgccg cgcgtggcga acttgaccag catgaggtcc gcgccgccgt
     1261 tcaggaactg cccgaacagc gcgttgtacg tggtcccgac cacgtacagg ttccgcgcgc
     1321 cgtcggcggc cgcttccatg ccgtaatccc cgagcgcgcc gccgaacagc cgcatccaga
     1381 tccgcgttcc ggacggcgtg aaggcggata cggccgcgtc gtaccagccg ctgttcgtct
     1441 ggccgtccag cccaccggtc gtatagccca ccgcgtacgc gttgcccgcg tcgtccaggc
     1501 agacgccgag cccgtagtcg ttcgtggccg agccccagat ccgcgtccag gcggccgtcc
     1561 cgccggcgtt gagcttcgtg agcgcgaagt cccagcgccc ggcgttggtc tggccgtgga
     1621 acgcgccgta ggagtagccg aacaggtagg tgttgccgcg ggtatcggag gcgatgtcct
     1681 cggcattctc gtccgacgcc gagccccaga tccgggtcca gaggctcgtg cccgtcgcgg
     1741 tcgagaggcg ggccgcaagg aaatcgttgc cgccggtgtt cgtctggccg gccagcgcgc
     1801 cggccgtgaa gccgccgaac atcgcgctgc ccgacgggcc cggcccgatc ccgcgcgcgt
     1861 gatcgttgct gaccgagccc cagatccgcg tccacgcgag cgtcccgtcc gccgagaact
     1921 tcgcggcgaa gaaatcggcc ccgcccgtgt tcgtctggcc ctcgaaggcc ccgtaggtgg
     1981 tccccgccat gtagacgaac ccgtcgctcc cgacgtgcac gccgttgccg tagtcgctga
     2041 acgaggagcc gaagaaatgc gtccattgat ggtcgccggc cgggctgtac ttggccagga
     2101 acgcgtcccg ggcgccggtg tacgactcgc catggaacgc gccttcggta taccccacga
     2161 cgtaaaggtt ggcgtctccg tccagacaca gttcctccgc ctcgtcgtgg ccggccgaac
     2221 cgaaaacgcg cgtccaaacg cccgagccgt ccggcccgaa cttgcggagg aagatatcgc
     2281 tgtcacccgc attggtctgc ccgtccagcg gcccgtagcc gtatccgacc gtgtagacgt
     2341 tgcccgcgcc gtccgcgcgg acggcccggc cgaactcgtt ggtggccgag ccccacagca
     2401 cggcgtcgtc cacggccggg ccgtacaccg cgcgcgccgt gcggttggtg gtcagcgtca
     2461 ccgcgacgct ccggttggtg tccgcggcgc tctcgtcgag cagccagcgg tggaacacct
     2521 gggcgccggc gttcgacggg gccgtcaggg tgacgacggc cccgttggtg tacgcgcgcg
     2581 tgaaggcggt cgtgcccgtg ctctcgcacg cgaggtcggc cggagcgacg tcgatcgcaa
     2641 tgccggcgtc gggcgaggag gagagcacgg tgagcgtacg ccccgtcgcc gtcccgtcgc
     2701 tgaccagcag cgtcgagccg cccttgtacg gcgaggcctc gttgctctcg tccacctcgc
     2761 cgcggcagtc cacgtccacc aggatccact gcgtgtacgt gccggatccc cggtcgggca
     2821 tggcgaactc ccatcggatc gactgcgcgc caccggggcc catcgcgccg acggactgct
     2881 ctcccgcgca gaaatcgtcc gtcaggtccc agtcgttgcc ggggctcaag tggaacgtgg
     2941 cgtggctggg gccggcggct ccctgaccga tgttcgtgat gatgcagttg atccagaacg
     3001 cgtcgccctc gcgcaggacg ttggttcgcc acgcgtggcc gctccagatc aggtcgggca
     3061 gggcaccggt gacgctgtaa acggccgtgg ccgaatggat tgagcctgtc gccagcgcgg
     3121 cgttgacgat gcgcgaggcg cccttgttcg tgccgtcgag gagccaatgg ctgaacggtt
     3181 tcccttccgc catttcgggc gcggtgagga cggccgtgag gttgctggtg taagcgcggt
     3241 tgaagggcgt gatgccgtcc gcgttgccgt tgcggtccgc gggagcgacc tctacggcga
     3301 cgccgccgtc cggatcctgc gacgcgacgg acagcccggc cgagagccca tacaccgccc
     3361 gcgcggtctg gttgctggtg agcggaacgg tcagcatcgc gttgttcccc gcgtcggccc
     3421 cattgagcag ccagtggtcg aacgggttgc cgtcggcgaa cgtgggcgcg gtgaggctca
     3481 cggtggtgtt cgtggcgtag ccccgggtca cggtggtcag gccgttcgcg tcgccgttct
     3541 tgtccggcgg cacaacgccg atgctgacgt ccacgtcggg attggacgag aacacggaca
     3601 gcacgcggac cgagccggtg ggggccagga tggaatagaa cggattgacg ctcttgaaga
     3661 ggttgttttc gttggtttcc gtcacctcgc tgtcggtatc caggtgcacg attgtccaca
     3721 cggcgttgga cgggaagggg aagtgcggcg ccgtgaaggt ccagcggacc caggtgtccg
     3781 tgttcgcgtt gagggcgggg atggggatcc ggccgatgag gaagtcgtcc tgcagggtaa
     3841 aatcgttact gggtgtcaga tacagccggg catggcaggc gccggcgtgg cccggcccgt
     3901 agttggtgac gctggcctcg acccagaacg gctcgccggc gttcacaagg gtcttgtccg
     3961 accggaacga acggaccgag aggtccggca gcaggttggt cgccgcgccc ccgccgccgt
     4021 acaccgccgt cgcggtgcgg ttggtggaca gggtgacgac cagcgagcgg ttcgtgcctt
     4081 ggtccgcgcc gtcgagcagc cagcggctga acacgttcgt gccggccgcc gccgccgcgg
     4141 tcagcgtgac ggccttgccc gcgacgtacg tgcgcgcgag cggcgtgctg ccgtcgccgt
     4201 tgccctcgtt gtccgcgggc gagacggtga cggccgcgcc ggaggacggg tcctgcgact
     4261 ggaccgttag ggtgaagtag tcgaaatgcg gaacggtgcc gaggatggcc gtgaagaccg
     4321 cctgggtcga ggcggggttg acgtcgtagg ccagcgccga ggtgacgtcg gaagggccgt
     4381 actcgggcgg agacgagcct ccgatgagcg gaagatggga cgtgttgatg tagccggcgg
     4441 ctgggaagtt ggtagcgcca ttgtaccgga tgtgttcctg caaggcgtca taccaatccg
     4501 catgccagcc ggcccacgtg acgttgcgct cgtccgtctt caggacgccc tggctcaagc
     4561 cgacccgcga ggcctggtcc acggtgtgca actcgagcga accgctggcg taggttccca
     4621 gcgggataaa cacatcatcg aaggtgtccc cgacgtcctc gtccacgtac ggcacgcacc
     4681 gcaggtcgcc gaggccgccg gcgcgggtca gggtccagcg ctgatagagt gcggtctctc
     4741 cggggggtat ccaggtgagg acggtgtact ggatcttgcc gtactcgcca tccagcgtgt
     4801 cggagcactg gacgcggttt gagccgagat ccacgggcgt ggcggaggga agcatcgccc
     4861 cgccatcgtt cacggagaaa taggtcagga actcgtaaag aaagccgacg gtccccgagt
     4921 cggcgcccac gtatccgtcg ttgcaatacc cgccgaagct aaccttgacg gccaggtagc
     4981 ctttggccga cggcgagagg ccgttgctga tccagatgcc ctgcgcggac gcgccggacg
     5041 ccaggccgaa caaggcggcg gtgataaacc atcggcaccc gcttttcata cgcgcctccc
     5101 cggcgaatga accgctcctt gtgttgcatt ttcgctccag ccgcctgtcc ccgcaagcct
     5161 ttttcaaagg atacgttgtg aacgcggcgc ttgtgtcggc ggtcagaatc cccggttaca
     5221 acagcggcgg cggggtctat actgttcggt gaaggagttc ggaatgaaag cggtgctccg
     5281 aggtctgggc gtggcggcgg ggctggttct gcttccgttg atcggcgtga agattcgggg
     5341 ctatcccgtg acccggtacc tggagtttcc gcccctgacc cgctacgtcg agcatgcggg
     5401 tttttcctgg cccgcgttcg tcgtgctgac cctgctcggg ctcgcactcg ttgtgccgat
     5461 ggtccgggtc cttgtccgcg cctgggccga ccgcccgcgc tcgaagccgg cgcggccgtt
     5521 cccggcctgg ggctggttcg gcgtggtctt cacggccgcc gcctgggtgc tggcgtggaa
     5581 ccgcttcccc tggttcgcgc cgctccagcg attcacgttc acgccgctgt ggctcggata
     5641 catcgtgctc gtcaacgcgc tggcgtatcg ccggacgggc gcctgcatga tgaccgcccg
     5701 gccgcgcttc ttcctcgcgc tgttcccggt cagcgcggtg ttctggtggt acttcgagta
     5761 cctgaaccgg tttgtgcaga attggtatta cacgggcgtc gagcacattt cgccgctcca
     5821 gtatttcatc gaggccacgc tcccgttctc caccgtcctg cccgcggtgc tggggacgtg
     5881 cgagtttctc ggctccgttt tcggcagacg tccggccgcg agcgagccga gttcgggccg
     5941 acccgcgccg gccgtgctgc tcctggcggt cgccgcgctc ggcctggcct tcctcgccgt
     6001 gtggccggac atgctcttcc cgctgctgtg gatctcgccc ctgctggtgc tttccgcgat
     6061 gcggatgctg tcggaccggc cgttcctcga tccggggccc gacgggagcg cggcccggcg
     6121 cgccgcgctg ttcgccgctt cggcgctgat ctgcgggttc ttctgggaga tgtggaattt
     6181 ccacagcctc gcgcgctggg tctacgcggt gccgttcgta aaccgtttcc acctcttcga
     6241 gatgccgctg ctcggcttcg cgggttacct gcccttcggc ctcgagtgcg cgctgatcgg
     6301 cgacgcggtg ggggcgtgga tcaggaaaaa tgacgaatga cctaaccggc cgccttgcct
     6361 gcgccgcccg ccgcgtgaac ctcggcgcgg ccgccgccat cagcctgtac tttttcatcc
     6421 tgcccgggct gtccgtcctc cgcgatctgg ccgatcccgc gctgcggtcg gacgccatcc
     6481 cgcgcgccgc gtggcggctg ttcgaagacc tcacgccccg ctacgagcgc tgggcgcgcg
     6541 gccggatcgg cagcggcaag gctgagtcgc tggcgctcca cgagatcgcc ggcacggagt
     6601 ggccgctgtt cgggtccgtc ttctacctct ggtccgtcga atcgttgcag gaggattggg
     6661 agcggcgggc gaatccggcc gggcccgcgc cccgcgagtt cgcccgcggc gcggtcgagg
     6721 ccgcgacgga cctggtcctc gaccccggcc acgcggcctg ggtcaggctc cactggggcg
     6781 acgactacct gcaccgcgag aacgtgttct accgcatgct gctgatctcc gcgatgacca
     6841 gccaccgccg cctcaccggc gacgcgcgcc accggcccat gttgcgcgaa caggtggaga
     6901 ccctctccgc cgaactggac cgctccccgc acggcatcct gaacgactac ccgggcgagt
     6961 gctatcccat ggacgtgctc atggccgtcg ccgcgatccg ccgcgcggac gccgtgctgg
     7021 agacggatca ttccgccttt gccgcgcgct cgctccgcgg tttccagggc gcacggctgg
     7081 acccgcgcgg gctgccgcca tacgtcgccg atcccgtccg gggcgtggcc ttcggcgccg
     7141 cgcgcggctg cgggatttcg tacgccttca tgagcgcgcc cgagttgtgg ccggatatcg
     7201 cgcgggactg gtacgcccgc tacgagcagt ggttctggca ggagaagtac ggcttgcgcg
     7261 ggttccggga attcccccgc gacctcggcg gcggggactg gtacatggac gtggacgccg
     7321 ggccggtgct gggcggggtg ggggtctccg ccagcgcctt cggcgtggcg ggctcgcgga
     7381 tcatgggccg gttcgaccgg gcctggccgc tggcggcgca gatgaccgtc ttcggctggc
     7441 cgttgccgaa cggcacgtgg ctgacgccgc gcatcctctc caacgcggcg aacgcgccgc
     7501 tcctgggcga ggcctgcatc ctctacagcc tcacgcgccg cgcggcgccg gggatggaaa
     7561 tccggaccgg tggatcgctc ccgccgatcg tggtgctcgc gtgggtggtg tatttcgggc
     7621 tcggctttct gttccccgcc gccgaggtcc atgccgtccg ccggtggcgc gcgcggggcg
     7681 cccccgtccg cgcgcccgcc ctgcaattcg cgatctggct ggccctcgtg cttgcggcgg
     7741 ccgccctggc cgttcgcttc gggcccaccg ccggcttcgc cgcgctgttc cccgcgctcc
     7801 tgttcccccg ctgatggcgg gtccctttgc tgttgaggcg cggcggtcct tcggctatcc
     7861 tgtggccgtc ggaaccgcac aaggagagcg cggaggaggg ggtatgagca gtctcattct
     7921 gcgtggaagc tccatcgcat ggaaggcctc tgttctggcc ggggcgacgg cctgggccgt
     7981 ccggggcgcg gtggcggaca cgctgttctt cacaaacgag gccgactggg ttgccgccac
     8041 cacgaaccgc gaactgttcc agaccaccgc ggccaacgtg ggcctggcgg acgaggtgga
     8101 cgcgccggcc cagaacaacg tggacgtggg ctccaacctg accttccagg ccgtgaacac
     8161 cggcctcccg tggtccttcc gcgtggcgac gctccaggcc ggcgcgcggt tcacgttcag
     8221 cgacaacgag gagggcgccg gctatccgcc ctacttggga ttcgaggaag ccctttcggt
     8281 cggcgacatc gacgactacg aggacgacga tttcgaggtc gttatcctcg acggccccaa
     8341 cctgcacgga gtggcgatgg acatcgggga gaacgatccg ctgatcgctg acggcggcgt
     8401 ctacgtctac gatcccgccg cccagttgat cggctattcc tccatcgtgc cggcccggtc
     8461 caacgccttt gcctttctcg gcatcgtgtc cgacgccgcc atcggccggg tcgtgatcga
     8521 cgaggatacg ggaggcgacg acgtatccgt ccggcacttc cgcttcgcgc gccaccgcac
     8581 ggccgatccc gcggtgaccg cctgggcgcc gctcgccggg gggcaggcgc gcctcgaatg
     8641 gccgtcggcg tcggattggc ggtatgacgt cgaggcctgc gcggacctgg ttggcgagtt
     8701 cgcgcccttc gcgaccaacc tgcccgccac gccgccggtc aacggcctca cgaacatcct
     8761 ggggcccggc cgcgcctttt tccgcgtccg cgcccgcgcg gagccgtgac ggcggcgtct
     8821 cgatgaaaga atggcttttt tgcccgccat tcgctttaat ggattcaagc agggaggaga
     8881 acggcgatga cggctttttt cgagatgctg accacgatgc agaagttcta cgcggtctgc
     8941 gccctgttcg gcgcgctcct gttcatcgtg cggacgatcc tgatgttcct cggccatgac
     9001 gacggcatcg atacggatca tcccgcggac atgtcgggcg acgccgacgc gagcttcaag
     9061 gcgctttcgc tccagggcct gaccgccttc ttcatgatgt tcggcctggt cgcgctggcg
     9121 atctcgaagc aggccggcgc gagcaacctg tgggctgtgc tcggcgggtt ggtcgcgggc
     9181 ctgttcacgg tctgggtgat cggccgcatc tttatcggca tgaagaagct ccaggccgac
     9241 ggcacgctgc ggatcgagac cgccgtcggg caggaaggag aggtctacct gcccctcgcg
     9301 cccggcaaga gcggacaggt ccgcgtcgcc gtgcagggcc agttgcgcgt gttcgacgcc
     9361 gtcacggagg gcggcgccga gttgaagacc ggcgcccgga tccgggtggt ccgcgtgacc
     9421 gccgggaacg tcctggtcgt cgagaagatt taaggaaaaa aatggaggga cccgccatgc
     9481 tgccgatcgt cgtgctcgcg ttcgtgatcc tgattgtttc gaccatcatg ttcctcgcgg
     9541 cgcgttacaa gcgctgcccg tccgaccgga tcctcgtgat ctacggcaag gtggcggata
     9601 accgctcggc gcgctgcatg cacggcggcg gcacgctcgt ctggccgctg atccaggact
     9661 acgcgttctt gagcctgacg ccgatgacca tcagcatccc gctccagaac gccctctccc
     9721 agcagaacat ccgcatcaac gttcccagca cgttcacggt cggcatcagc gtggatccca
     9781 acatcatgct caacgccgcc gagcgcctgc tgggcctgga gagcggcgat atcgaggcga
     9841 tggccaagga aatcatcttc ggccagctgc gcctgaccgt cgcctcgctg accatcgagc
     9901 agatcaacca ggaccgcgaa agtttcctgg agtccatccg caagaacgtc gagccggagt
     9961 tgaacaagat cggcctctac ctgatcaacg tcaacatcac cgacatcacg gacgagtcgc
    10021 tgtacatcga gagcatcggc aagaaggcgg ccgcggaggc ggtcaacaag gcccgcgtgg
    10081 acgtggcgga gcaggacaag gtgggcgcga tcggccaggc cgaggccaac cgcgagcaga
    10141 ccgtgcgcgt ggcggagaac gtcgcggccg cggagaaggg caagaagaag gcggaggccg
    10201 accagcgcat ctacgtccag ggccaggagg cccaggccgt cggcggcgag aacaccgcca
    10261 aggcggacat cgcggattcc aactccctgc tggccgtgaa gcaggccgcc gcgatgcaga
    10321 aggccgaggt cgcccggcgc gaggcccagg tccagattca gaaggcccag tacctcgccg
    10381 agcaggagcg cctgaacgcc gagctggtcg tccagcgcga gatcgagaag cgcaacatcg
    10441 agatcatggc cgaggccgag gccgagcgca tccggcgcga ggcgaagggc caggccgacg
    10501 gcacgctgct gaagtacgag gcggaggcca agggcgtgcg gctggtgctc gacagcaagg
    10561 ccgccggcta caccgcgctg gttaagagct gcaacggcga cgccaaggcc gccgcgacgt
    10621 tcctgatggt ggagaagatc gaggcggtcg tcgcccgcca ggtcgaggcg atccgcaacc
    10681 tgaagatcga caagatcacc gtctgggatt ccgcgggcgg cggggagaag ggctcgtcca
    10741 ccgcgaactt cgtctccagc ctcatcaaga gcgtgccgcc gctccaggac gtcgcgtcca
    10801 tggccggcgt ggaactgccc gagtacctcg gccggatgac cgaggacaag gcgaagaaga
    10861 agccggccgg ggaggcgtag tccgatatcc tgacggtccg ctgatcccgt cggcaaggcg
    10921 cgtttttcag ccctggcgat agcggccgtg ccgcctatcg aatcttcgct taacagaaaa
    10981 gcgaaaccgc ggtagattag tcgagcgttt taccttcgtt gtttagttcc aatatgggtg
    11041 agtccgtcct tggacgggag aagggatagg gagaggaatg actatgaaaa ctcgaacctc
    11101 tgcgctgggc gccgtcctct ttctgtttat ggcgggcacg agtctcgcgg cttcgagcct
    11161 gtcgatcttc ggcagcgacg ccgagaaccg cgtctaccac ttcttcatcg acgagtccgc
    11221 gggcgagacg aacgtcatca cgttcgagtt ctggccgaac gacggcacgc cgaccgtcgc
    11281 ggagatcttc acgacgctca accaccgcga ccaggccacg ctctccccgc ccgaccccaa
    11341 caccgtggcg cagggcgacg cgtccggcta ctggggcgcc tacaccatgg ccgacgccgg
    11401 cggcggcaag tggacgctcg ccctcccgat ccagaagtgc ggctcctatg acgtccgcgc
    11461 ccgctacaag gtctcgggcg acgccaactg gcgctggtac gccggccgca atcccgtggt
    11521 caacatctcc gacgtcgcca cgcgcgacat ggtggtctac gagatgcagg ccaacgtcgt
    11581 ggacgccacg ggcaacgact acgccacgcg cagcacgttc gcgtcgctga ccaactcggc
    11641 gaagaactgg aacctggact acatcagcgc cctgggcgtg aacacgatct ggctccagcc
    11701 gttccacccc atcggggcca agagcgactg caactccggc gaccccggca gcccgtactc
    11761 gatcaagaac ctcttccagg tggcggagca cctcggctcg gacggcacgc gcgccacggc
    11821 catgcgcgag ttcaccaact tcgtgatcgc cgccgacgcc aagggcgtga aggtcatctg
    11881 cgacgtgatc ttcaaccacg tggccacgga catcgagatc gagcgcgatc ccgacaaccc
    11941 gacgaatctc tacgccaacc cgctggccga gatgcgcagc gtgaagtacc cgtggttctc
    12001 gcgctacacc ggcacgcgca gcggctgccc gaccaccaag ccgcagtccg actgggcgaa
    12061 ctaccactac acggagaccg ccacgagcgc gtcggagatc ggcccggcgc cggcggaccg
    12121 caacgacttc gtctggccgg acgcgttcga cctgttctgg ggcacgtacc cggcgctggg
    12181 caacatcaac gacacgagcg acggcgcgtg gacggcctcg gccgacgtga agaagatggt
    12241 ggagtactac tcgtatttca tccgctactg gcacgagaag acgggcggca agatcggggg
    12301 attccgctgc gacttcgcgc agggcctgcc gcggcaggcg tggcagtacc tgatcaacag
    12361 ggccaagtcc ctcaagccgg agctctactt cgtgtccgag tcgctggacg gcggcaacat
    12421 cgcctatcgc gcgtggaagg gcgggttcga cgcgctgaac gagaatgagc tctgggcgat
    12481 cgtggaggac ggcgacatcc agtcgaccga cctgcgcaac gtcatcgacc agcgcaagac
    12541 gcagttcggc ctggcgctga tcctgcgcgg cacgatgaac cacgaccagg gcccgtggat
    12601 cggccgcaag tgggacgggg tcgcgatgca cagcgtcttc tgctccgtgg acggcacgcc
    12661 gcagttgtac gagggccagg agctgggcta cgacgcgctg gggcagttca gccgggagcg
    12721 cgtggagttc gggcggacga tcccggacat ccggaactac cacaacatga acaacctgtg
    12781 gtccggccgg tacagcgggg acaacgagtc gctgtggcac cgctacaagg acgcgaacct
    12841 cggccggtcg cggtcgctcg cgttgcgggt ggcggaccag tactacctcg accgcaccgg
    12901 cggccagggg ccccatccca agatcttttc cgtcctgaag tacacccgct acgggtggga
    12961 cccgaaggac caggacgtcg tggtggcgtt cgtcaacctg cagccgggca cggccaacag
    13021 cggcacgttc aacgtcaacg tcgcgccgat ctacctggat ccgacccgga cgtacaacgt
    13081 gaagaacctg gcctcgacga cgccggacac gtacctgtgg gcgtcgggcc gcagcggggc
    13141 ggacatcgcg gcgaacggca tctacgtcgg cttctccgcc gacaagggcg cggagggctc
    13201 catcgcgcag ttcctcaagc tggaggagca gggcgggggc ccggggccgt cgacgaacct
    13261 gcagtggatc ggcaacacgc ggcactggcc cgtcaacggc tcgatcacgg cggcggacga
    13321 cctgtggatc gacatcgagt cctggccgac caacacggcc accggcggca cggtggtgta
    13381 cagcaccgac ggcgtgggct ggaacaccag ggagttgagc accaacggcg tggccggcaa
    13441 caacaacgcg tggcacgtga acctcggcca gttctccgcc ggcgcccgca tccagtacgc
    13501 cgtgcagatc gtgcagtcgg gcggcaccaa cttctgggac aattccggcg ggaccaacta
    13561 cctcgcggtc gtgaattcgg gcggcagcgg ccagcccctg ggctggatcg gcgcggtctc
    13621 gcactggccg acgaacggcg ccatcacgtc cgccgacgac ctctgggtcg acatccagtc
    13681 atggccgacg agctcggcga tcagcgggtt cgtcgtctac agcgccgaca acggccagac
    13741 gtggccgacc atgccgctct cgctcaacgc cacgtcgccg agcaacgact tctggcatgc
    13801 caacctcggc cggtgctcca gcggcgcgtc catccagtac gcggtcagcc tctgggacga
    13861 cagcggcacg aaccagcact gggccaacgc cggcggcacg aactactggg cgttcgtcgg
    13921 cggcaccggc agcacgcggt cggtgcactg ggcgggcaac acgatcagcc gctcgctgcg
    13981 ggcgccctgc ctggcggccg tgagcctctc cggcgacggg ctgaaggtgg agtcctcctc
    14041 ggcgatcgtc ggcaccgagt accggatgta ccggtccacg aacctggtgt cctgggacct
    14101 ggtgacgaac gtcgtggccg gcggcagcac cttctccgtc accaatcctc cctcggacgg
    14161 cccctccgcg ttctaccggg ccgtcgggca gaacgtcccc tacgaggtga cggtgttcga
    14221 gggcgacgac ctaatcgtgc gggccgaatc gtggccgcag ggccgcgcgg ccggcgccaa
    14281 catcgtctac agctcgaacg gccagaactg gtacgggatg agcatgacct ggatcggaac
    14341 gagcctgaac aacgacctgt gggaggccaa cctgggcttc ttcccgaagg gcatcagcgt
    14401 ccagttcgcg atcgaaatcg tggacgacca gggcttcacc ctctgggaca acaacggcag
    14461 ccagaactac tccgtggcca tccgcgaccc ggcccagccg gacgtcgcgg cgccggtcct
    14521 gtcgtactcg ccctccaaca cgacgaccgc ggccgccttc ctcgacgtga cgctcgcggc
    14581 ggtcgacgac tacgacgccc acccgctgat cacgtacacg ctcgacggct ccacgccggt
    14641 ctgggacagc gagctctaca ccgcgcccct gcacatcacg aacgacctga ccatcaaggc
    14701 catcgccatc gacgcgtccg gcaacacgtc cgccgtcacg tccgtctccg tgaaggtcaa
    14761 cgacgtcgtc gagctcggcg cgttcaagcc ctactcggtg aacccgacgc tcgggcgggc
    14821 ggcgaccatc accatcgacg gcagcccgtc cgactggacg accaacatgc tcgtcgcgct
    14881 ggacgtggcc gacgacgacc cgcggaccct gggcgacaac tggaccacgc acgaggctcc
    14941 gctcgacttc gcctacctgt gggccgcgtg ggacgacacc tacctgtacc tcgcgtggca
    15001 gtacgtggac ctgacggata tcatcgacgg ggccaacgcg ggctccgcgg ccggcggaaa
    15061 gatctcgagc aatgacggca tcctccagtg gatcgccatc gacacgatcc agggccaggg
    15121 cgcggccaag gacgtctgga gcaagaacgg cgggcagccg tactggaccg gcaccgaccg
    15181 gcccgactac caggtctaca tggccggcag cctgtggcag ggctacatct cgcgcgcggt
    15241 caacggcgtg ttcgcgctgg acgacggcgg ggtgaactac aagacggcgg ccgccgccgg
    15301 catctcgtac gccaagggca gcaccctcgg cgccggcacg ctgtggatgc cgtcggcgca
    15361 ccacgccgac ggccgcttcg gggcgttgag catcgccacg cacgacacga cgcgcgacag
    15421 cttctacgag atccggatcc cgttgagctt cctccaggtc acgcgcgccc agctcgagtc
    15481 gaacggcatc ggcgtgatga tgggggcggg ctcggtctcg tgcatggatt ccatcccgca
    15541 cgacgagacg acgctcgaca cgcccggcgt ggaggtctgg aactcctcca aggagtggac
    15601 ggacaccgac cgcttcacga cccgcttcgc gcgcgtcggg caccagaaat aggccggcac
    15661 tagagccggc ctttccgctt gaagtagagc gcgaggatgg cgggcagccg cagtaaccat
    15721 ttcccggcca ccacgagccc tcgggggctc ggaatgaccg gcccaccctg catcagcgtc
    15781 ccgccgtcgg gctggaggcg cggatcggat tcgaaccgat gcatagaggt tttgcagacc
    15841 tccgccttac cacttggcta ccgcgccgac ggccggccaa agatgcatgg acaacatagc
    15901 agacgggcgc tagaattcca gcgaaaagcg ggacgagtcg tgattcgaag atcatacctg
    15961 ttgcgccggg aactggcctg cgccgcggtg ctgggcggtt ggtgggcctc cggttgccag
    16021 gcgccgccgg cctccgccgt caactcggag ccggaacagc gggtcgcgac gctccgggtg
    16081 ctgggcgcct cgcacgagct gcgcgaggac caggtctacc cggcgccgga gacccgccat
    16141 gtgctgcgat ggaaggccgg ccgcttcgaa tggcagatca cgctggccga tcgcgcggaa
    16201 ccgtacgcgg ccgtgctcct gcagaggccg ctcgacctgc ggcgctaccg gccgaagtcg
    16261 tacctccgat tccgcatcca gcccgatgtc gcggccgccg acctgtacct cggcctcgcc
    16321 gaccgccgcg cggacgggac cgatcccgtg atggtgatcc tgccgctggc cccataccgg
    16381 acctggcagc gcaacgcgct ggggtattac gcgatcccgc tgaaagcctt cggcgaccgc
    16441 ggatgggtcc tggcttcgaa ccggcagagg gcgccgtcgc tccacgcggg cgcgatggac
    16501 tggagccggg tcggtgaatt ccggatcggg acgcagggcc ggccggcatg gcgcaaaccg
    16561 ttcgtcgtca gccggttcga aatcggcgtc agcccgcgcg tctccgagga gtgaggccgc
    16621 cggtcagccg cggatcacgg cgaggacttc atcgcggtgc cgcgccatga ggtccgccgt
    16681 tttcgcctcg aggttcaggc gcaccagcgg ctcggtgttc gaggcgcgga cgttgaacca
    16741 ccagtcgtcg aattccacgc tcaacccgtc cagttcgatc atccgcccgg cggcatattt
    16801 cgctcgcagg cggccgatga cgcccttgac gtcgtggacc tcggagttga tctcgccgct
    16861 ggcgaagtac cggcggatcg gcttcaccag ttccgacagc ggccggtccg attggctgac
    16921 caggttggcg atatacaggg cggccaggga cgcgctctcg gtgtacccgt tctcgcggaa
    16981 gtagtagtgg cccgaaagtt ctcccgcgaa gagggccttg gcctcgcgca tctgcgcctt
    17041 gatgaacgcg tggcccacgc gggacatcat gggcgtgccc ccgttttcct cgatgacctc
    17101 cttcaccgcc cagctgctgc gcaggtcgta gaagatcggg cccttctcgt gctggaggat
    17161 cgtctgcgcg atgagcgccg tgatcatgtc catcgggatc acgttgccct tctcgtccac
    17221 gaaccccgcg cggtccgcgt cgccgtcgta ggccacgccg aaatcgtggc ggccggagcg
    17281 cacgagcttc tgcaggggct cgagcgtatc cagcttcag
//
LOCUS       DAOOTK010000058        17267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2269_length_17267_cov_5.238787, whole genome shotgun sequence.
ACCESSION   DAOOTK010000058 DAOOTK010000000
VERSION     DAOOTK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17267
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2269_length_17267_cov_5.238787"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..9770
                     /locus_tag="PK634_08015"
     CDS             12..9770
                     /locus_tag="PK634_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97717.1"
                     /translation="MDTGVYTSFTGITIGQLDAGDYSNQVRTVIQGSTNEATGGSILT
                     FVSTATGITLEQAEAMELRNLTIRGAQTGVSLSQARRSLLEWLRIEGGNIGFSLASAE
                     GTILRHCLVRDATLTGVYNTSADIAWENGVLWSNRYGTYLAGGVLGFRDSVVAVFGTN
                     AAAHYISSYSVLQADYNALFLNEGALAGLLPRVTPWPDQYFSVANWAAATSNDTHSLA
                     CDPQFADATGGNFRLKSVAGRYSPAITGYVADATTSPLVDAGDPAAAWTNEPLANGSR
                     RNMGMYGHSAQASQTPTNAALTVITFDDGGYGAGIIPLYWLAQGGATSHTARLRYSSD
                     GGAHWQYVATNIAAGAGVYYWDSTTFTSSIRGVWWIQSEQDSLLQDTNQVFFALRNET
                     LRFYVDDASTNGNVYCSAPGNPNHTGTTPADPLDKVQSVLDRWDLEPGDWIYVDTGEY
                     ILSDRIEWDRFDAWQNTNLVYLAQDLDGQQVVLQGSTNEPAGGSRLIRENDNDYLLYL
                     NTAPGVMLRNLTLRNGLAVLRADSSAYGGAEWVRFEEGRIGVDLHDCRAFEMEHCVSR
                     NHAAQGLSVVSSEGTTWRSGVIWSNQIGAYQYQESGKHSSLTLEHTVIGAFGGQSYAL
                     NHISGSWTSDYNNIYVTEGAWPAGMAAWPSPTARTNRLEAVFYWTREMETDYHTLTED
                     PGFVNAAGGDFHLQTTRPGGRYDPILGIWTNDAGFSRIIDAGNPLASFSKEPNPNGRR
                     IDIGLYGNTAQASKTPTNAWINLITLHDGGSVQGSQWLYWVAGGSATGLNVYLDFAHV
                     SGIDMWTNIATNAAASLGENEWNSSGYGRSAAGEVRIVASEDRSVNATSQVPISLRDE
                     TGSIWYFVNNTDTNGDVYTTVPGKSSYPGTTPYLPKASIQDVINTYKLEPVDIVFIDT
                     GEYKLTADIQLDDLDTGSGTNLVTFQGSTNWAAGGSVLNRQVSVGTAVMKLRQVSGIQ
                     LKDLTLRGAAVGLEVAYSDSCLFDNVRSRNNGSTGFQLQESDGIQFRHCIAWNNGGTN
                     GVGIASDQSSMSWSNSLLWANATGAQLTQAGTHFFRNSVLYAFGAGRRIFDLDTETSL
                     SDVDSDYNNLVLGSGATIAEKDQVFGGSDLYGTLIDWQTRTGIDPHSLSHESQFADTV
                     NGDFRLKSQAGRFRLNGTLTNDTVTSPLIDTGDSVTTWTNEPDPNGSRVNIGPDGNWS
                     FASLSRTNPWLLAVSLNAGGTIRGTNTLRWTSGNIATDALVRLEYSRNSGVEWRLIAS
                     NIVNGVTGYTWNVSAETTTVQGAWRVIYQADTNVWDRADVDFIIKNGTVNIYVNDDST
                     NGDVYCRGLTALGNDGQSGLTNTQPMRTPSYAIARYALGPGDTVYIDTGTYNMSSNLY
                     LGELNRGTETEPIRIFGSTNWAAGGTLLNRGNAFEPGIRLQNTHYVELRDLRIMDALD
                     GIEIDNVIGAVLERIEVFKSKNGISTRTLRDGRLDQCVLWNNRNWGLTALSQGTLTFQ
                     RGLFWSNRTGAIQLQSSSLAISNTIIHGQGTSQLYQISEAVPNSDFNILWKEENAILV
                     RDTYNEVTYPNLQSWQRNLNVDEHSVLLNPMLFMPTNGDFHLMSDAVSGRYSRASGRW
                     TNDASHSWAIDAGHPSAPYVNEPNPNGTRMNAGHFGNTWEASRSSTNKALLATSFNDG
                     GLVSGSDVLYWLSRGLTASDLVRIEFSQNNGLEWTVVASNRPATEAGYEWDTSSYPST
                     PLALWRVVLQADTNVADAVDRTFILRNGPIMFYVNDASTNGDVYTRMPGSAAYDGLST
                     NTPMNSLQGILIYDLEAGDTVWVDTGRYTNYAPIEINTLHSGASTARVHIVGSTNVPA
                     GGSVLIWDYSECCPGNGITFRNASWIEMSDLEIRDVETGLQFELYSGNNLIRNVQIYN
                     AEEYGVYVSLSSGNQFKNLLVAGRSGKGLGVSSSPSVYLDSSVIWGNEDSAIEAGTTA
                     LYLTNSVLFASSSSNYCLRLFTNTTVTADYNCYFSTNAAPCAFYNGVVYDKLLRWTEA
                     SAQDVHSLNEDPLFANATNFDFHPRSVIGRYQRGTGWTSDADHSPLIDAGAPTWPFDL
                     EYEPNGGRINIGLYGNTVEASNSRTNEWLRALTGSSGGRISGLFSLAWAGWTFAPTNT
                     VRLDYSYDNGGNWTTVVEHVLLTNWSYLWNSTDAALSPICRWRVVLESNTNIWDINDF
                     FFGINGPFRFYVNDTNRTNDVYTTAIGSDTNFGLYPHIPKATLNNVLETYDLEGGDAV
                     WVDSGVYIITTNNPIVWPAGDSGSPGYPVYLWGNTNEPLTILRGQWAPSPLLANLTFQ
                     ATDLRVAGFNCQDGHMSATGYRLVFDDLEFSNAWFYMIGGAQTARTVRLTAGTVSVAG
                     TGLGLHRFAVREGNIVAGTNAVELINSLVTGGSNPAVSFSGTDLLLRNNTLVGTGTQI
                     RKAGTGNVVIENNIISASGLDRFCILLEDGTMESDYNNLYAVSNAWIGNQDGYWERLI
                     YWQEESGQDLHSVATDPYFADATNGDYHLRSAAGRWTDGGWTNDAVHSPCIDAGHPDS
                     VATNEHLPNGYRVNLGAYGNTEEASWSVTNAWLRALTMNDGGVLRGTNIIRWTSGWLG
                     AGDLVRLEYSTNNFASSYTIAAGISATDGQYIWDTTLVPSSLSAGWRVVLETNTAVEG
                     RTEQPFAVRNSPLPFYVNDASTNNDVYTLQPGSASHDGQSQTTPKDSVKGILDAYDTE
                     PGDLIYVDTGSYTSPVDTRIIWSRGGEALRGSVLIQGSTNYAAGGSVLTRSSLPGTML
                     FDVKASYVSLRDLAIQGANRGIFLESNRFCSLERLMLRSNNYGVVLHGAVGTTNRNLG
                     FSNNRLGGMDILDGRTTLVEFCTFAGNSNFSLRVDDSVANTLQNNIYYLNTISTTNYA
                     GDSNALYSAVIDYNVYHFVTQLVVRAVTNILPVPPDSAITNIVVFTNIEPLCIYGSYS
                     NLQAWQLAEVHDYRSSITNPMLADADRGDFHLRSTTGRYVAGWGWTNDTVNSWALDKA
                     NPASSWTNEPWPNGARANLGMHGNTEQASKGGVATQTLVRTFNQPVYVSETNSIMPLI
                     WHVRNVPTGAVLNVQYSGDYGTNWITILSGVNAYLEYYLWTPTPYYNTYKGKWRVVGA
                     FETNYWDMNDDIFNIFYGEFAFSKVRRDPTLTEVTWRGAWAEEYQLQYATNTTPTTNI
                     VATFIYTNMVGTNVLIRTNVVREILINWTNAPSGSGTNEQANFVSPNGGDLLYVDPTS
                     TGAPPGRLYRVIDKRSAP"
     gene            9767..10675
                     /locus_tag="PK634_08020"
     CDS             9767..10675
                     /locus_tag="PK634_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97718.1"
                     /translation="MTVGRRALVWMAVLAALLAPVGQGSEDRLMAGLRDALHFPLFAL
                     LTWSWGGRFLPCWRTAAVMTLAVLGVEAVQPLVGREAEWRDALFGLAGVVMAMALQGM
                     RQPRHATFWKALMIVAPAIVLCPLLIVGADRWEARRAFPLLAAFRSPWEIGRWTGRGC
                     DLRRVRRDGAWAMRVAVRNTAPAYPGAFLMEAPRDWSGMAALQVAVCWPGPDERELWA
                     RADDRPGSPPYSDRLQKALAMRPGTNRWVISFEEWKSTPGGRPFDFRRVRSLGLFFAE
                     AAPGEALDILEIRLVPASRPPAGAAQ"
     gene            10741..11436
                     /locus_tag="PK634_08025"
     CDS             10741..11436
                     /locus_tag="PK634_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97719.1"
                     /translation="MKLSIIIPVYNEEATVEKLVELVESVAVPTEKELVIVDDCSRDR
                     TREILGELKIRHPNIRLVFHEKNQGKGAALRAGFAAAAGDILLIQDADLEYDPREYPR
                     LLAPILDGHADVVFGSRFLGGGPHRVVFFWHYVGNKILTTLSNMMTNLNLTDMEVCYK
                     VFRREVMRGITIRENRFGFEPEITAKVARGRWRIYEVPISYYGRDYSEGKKITWKDGF
                     RALWCVVKYRFAS"
     gene            11446..11823
                     /locus_tag="PK634_08030"
     CDS             11446..11823
                     /locus_tag="PK634_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97720.1"
                     /translation="MTEPNKVEIICSACGAEALLMREPRYEGFRRIGETLTCSACRHE
                     YPSEEEVPFKAAARPVVFSDADRPAPVRLFQEGEADRLCRHCLHYVVNPFMQWCGVHR
                     KEVEATDTCSRFKRKPDPKPPPP"
     gene            11885..13276
                     /locus_tag="PK634_08035"
     CDS             11885..13276
                     /locus_tag="PK634_08035"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOW97721.1"
                     /translation="MAELLIRNIHTLVTMDAKRRVLRDADIRLEDGRVAEIGSRLPAR
                     PGARELDGRNCAAYPGFVNTHHHLYQTLTRNIPRVQNAKLFDWLVNLYEVWRELGPEA
                     VEVSTRVGVGELLLSGCTTTTDHFYVFPRGAPQEFLDIEIETARKMGVRFHPTRGSMS
                     RGRSQGGLPPDDVVQTPDEILADCDRLISKYHDPKPFAMCRIVLAPCSPFSVTTELLA
                     ETSQYARTKGVFLHTHLCETKDEEDFCQKTLGLRPLDYMEKTGWVGPDVWYAHGIYFS
                     EAEIKRLAETRTGVAHCPASNLRLGSGIAPIPKMLEAGVRVGLAVDGSASNDASNMVR
                     EMQLAMLVHRVGTGVEAMPPQKVVEMATLGGAAVLGQPEIGSLEPGKAADLALFRLDR
                     LDYAGAMADPASALLFCGAGPRAEYTIVAGRVLVEKGRLVGEDEEALTARANAVAERM
                     LRAAEEKRGVLYR"
     gene            complement(13273..>17267)
                     /locus_tag="PK634_08040"
     CDS             complement(13273..>17267)
                     /locus_tag="PK634_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97722.1"
                     /translation="TDPLQADSDGDGMPDDWELANQQNPLSNDPSLDPDTDGLTNLQE
                     YQAGTDPRDADTDDDGLSDGDELTVHLTNPLQADTDSDGLPDGWEIGHGTNANAHDAA
                     DDPDSDGLTNLQEYGRGTEPQQADTDADGMPDGWEVNQYFNPLVANGSTDADSDGLTN
                     LQEYQAGTDPRDADTDDDGLSDYQEAAIHHTNPLDPDTDDDTLPDAWEAGTGLDPLSD
                     AGLNGADGDPDSDTLTNLQEYLLGTHPMNNDTDSDNLTDSAELNLHGTDPRVADTDSD
                     GLPDGWELQYGFNPLSGIEAREQLACWLKFDEASGTTLVNSASAEWRATARGMPGSSR
                     TNGVRGGALVFDGLDDYVAIVQTNLPIVGGPGFTIAAWVWYDPAQPKDFATVCSDSRW
                     VGDKWPGFLVRADKTSRRFSGLVGNSTDASREVGLAEWPDRYAGRWTHVALVQDNGLT
                     RLLINGCIVGEQSNRFESAARPEVWLGQGHVSSTNSGWKGRLDEVRIYTTALSTEDIH
                     ALFEAEADANGDGISNLEAARAGLDPRSPTASPAVEGALDITFVPGAWTTNDPLQYLA
                     FFDDPNPSHEIHLFVERDTLEFTMYDAGGARHSIRHRDLLGGGHLWLHQTNRITASWR
                     GFNSGADTLEMRLFLNGVERNLTLGTRVNNPRLTMYNWEQGDGYYQAAYIRGEWSAAV
                     PSNRVWFGSRESGAHPLNATIVRGRLHTDAFGMVSTNPTPSFSEIDKTAPAAGRPQAL
                     LQSITRPGDIGDYIGPEEMTVLIRRYKQVADAVECEMAWLGEGDQSPSTWDILEDNIR
                     TTIEVGNQEGLDVALSSWFHFDSKICRKYTNSIPQWAERIQIIPEGDVGRAVLAPVNW
                     MHDGKYMATTFDVGDRATVSNFLAAWRQDLSMYSDYSYFLFNEDSLQWVGDPYTDSPT
                     YSSNALAWFREYTTAKYGPAYASIRFPISPIPVRAPDETNPPSYSRVTLDDSVTNRLE
                     LSIDPDLWAKWWEWRYVVFAHLMEGYTRQLAELNSGNYRWKGSIYLASPSVGWALNSG
                     VDLRLLARIPHLDWIVMENTRTYSYGTSPSRTEDEIRLQLQYVKDLASTNLGFGSYVM
                     AQAYPYPTVTNGVTNFTYNITWLTQDVAHAASTSFNAQIVVPYSASVLVNRPGFTSEF
                     QHATYVPEVAEAWLRERFLRLWTPISSLYPNGGTATYASVFFSWSYIDQSQGYEWQLS
                     TSPDFAVTNVEAQAYSSYHGWSILSDPMPTGQALYWRVRSVFHVYRYSDEGAVTGTNV
                     YYGAWSSDTNAPLYLQDDDLDGLPDAWEMYYFNSLIWEGDDDPDGDLVPNSEEYLDST
                     YPGGSGSIGP"
BASE COUNT         3013 a         5707 c         5708 g         2839 t
ORIGIN      
        1 acatcgtcta cgtggacacc ggcgtgtaca ccagtttcac cggcatcacc atcgggcaac
       61 tggacgccgg cgactacagc aaccaggtgc ggacggtgat ccaggggagc acgaacgaag
      121 cgacgggcgg ctcgatcctc acctttgtgt cgacggccac cggcatcacg ttggagcagg
      181 ccgaggccat ggagttgcgg aacctgacga tccgcggggc gcagacgggc gtgtcgttgt
      241 cccaggcgcg gcgctccctg ctggagtggc tgcggatcga aggcggcaac atcggcttct
      301 cgctggccag cgcggagggc acgatcctgc ggcactgcct ggtccgggac gcgacgctga
      361 cgggcgttta caacacgtcg gcggacatcg cctgggagaa cggcgtgctg tggtcgaacc
      421 gctacgggac ctacctggcc ggcggcgtgc tgggcttccg ggacagcgtg gtggcggtct
      481 tcggcaccaa cgcggccgcg cactacatca gcagttactc cgtgctccag gccgattaca
      541 acgcgttgtt cctcaacgag ggggcgctcg cgggcctgct gccgcgcgtg acgccgtggc
      601 ctgaccagta tttcagcgtg gcgaactggg cggccgcgac cagcaatgac acgcacagcc
      661 tggcgtgcga cccgcagttc gcggatgcga cgggcggcaa tttccggctg aagagcgtgg
      721 ccggccgcta ctcccccgcc atcaccggtt acgtcgccga cgcgacgaca tcccctctgg
      781 tggacgccgg ggatcccgct gcggcgtgga ccaacgagcc gctggccaac ggctcgcggc
      841 gcaacatggg gatgtacggc cacagcgccc aggccagcca gaccccgacc aatgcggcgc
      901 tgaccgtgat cacgttcgac gacggcggtt acggggctgg gatcatcccg ctctactggc
      961 tggcccaggg gggcgccacc tcgcacacgg cgcgcctgcg ctactcttcg gacggcggcg
     1021 cccactggca atacgtcgcc accaacatcg cggcgggggc cggggtgtac tactgggatt
     1081 ccacgacctt cacgtcctcc atccggggcg tctggtggat ccagagcgag caggactcct
     1141 tgctccagga caccaaccag gtcttcttcg cgctccggaa tgaaacgttg cgcttctacg
     1201 tggacgacgc gtccaccaac gggaacgtct attgctccgc gccgggcaat cccaaccaca
     1261 ccggcaccac gcccgccgat cccctggaca aggtccagag cgtcctggat cgctgggacc
     1321 tggagcccgg ggactggatc tacgtggaca cgggcgaata catcctgtcc gaccggatcg
     1381 agtgggaccg cttcgacgcg tggcagaaca ccaacctggt gtacctggcg caggacctgg
     1441 acgggcagca ggtggtcctg cagggcagca ccaacgagcc ggccggcggc tcccgcctga
     1501 tccgcgagaa cgacaatgat tacctgctgt acctgaacac ggcgccgggc gtcatgctgc
     1561 gcaacctgac gctgcgaaac ggcctggccg tgcttcgggc ggactcgtcc gcctacgggg
     1621 gcgccgagtg ggtgcgtttc gaggaggggc gcatcggagt ggatctgcac gattgccgcg
     1681 ccttcgagat ggagcactgc gtcagccgca accacgcggc gcaggggctc tccgtggtgt
     1741 cctcggaggg caccacctgg cgcagcggcg tcatctggtc gaaccagatc ggcgcgtacc
     1801 agtaccagga atccggcaag cacagcagcc ttacgctgga gcatacggtg atcggcgcgt
     1861 tcggcggcca gagctacgcc ctcaaccaca tctccggctc atggacgtcg gactacaaca
     1921 acatctacgt gacggaaggc gcctggccgg ccggcatggc ggcgtggccg tcgcccacgg
     1981 cccgcacgaa ccgcctcgag gccgtcttct actggacccg cgagatggag accgactacc
     2041 acaccctgac cgaggacccg ggcttcgtga acgccgccgg cggggatttc cacctgcaga
     2101 ccacccggcc cggcgggcgc tatgatccga tcctcgggat ctggaccaac gacgccggct
     2161 tctcccggat catcgacgcg ggcaacccgc tggcctcttt cagcaaggag ccgaacccga
     2221 acggccggcg catcgatatc ggcctgtacg gcaacactgc ccaggccagc aagaccccga
     2281 cgaacgcctg gatcaacctg atcacgctgc atgacggcgg ctccgtgcag ggctcccagt
     2341 ggctctactg ggtggccggc ggaagtgcga cggggctcaa cgtctacctc gacttcgccc
     2401 acgtcagcgg catcgacatg tggaccaaca tcgcgaccaa cgccgcggcc tccctgggag
     2461 agaacgagtg gaacagcagc gggtacgggc gcagcgccgc cggcgaggtc cgcatcgtgg
     2521 cgagcgagga ccggagcgtc aacgcgacct cgcaggtccc gatttccctg cgcgacgaga
     2581 cggggtccat ctggtacttc gtcaacaata ccgacaccaa cggggacgtg tacacgacgg
     2641 tgcctggcaa gtccagctac ccgggcacga cgccctacct gccgaaggcc tcgatccagg
     2701 acgtgatcaa cacgtacaag ctggagccgg tggacatcgt cttcatcgac acgggcgagt
     2761 acaagctgac cgccgacatt cagctggatg atctcgacac gggctcgggg acgaacctcg
     2821 tcacgttcca gggcagcacg aactgggcgg cgggcggcag cgtgctgaac cgccaggtca
     2881 gcgtcggcac cgcggtcatg aagctgcggc aggtctccgg catccagttg aaggacctga
     2941 cgctgcgcgg ggcggcggtc ggcctggagg tcgcctactc ggacagctgc ctgtttgaca
     3001 acgtgcgctc gcgcaacaac ggatccacgg gcttccagct gcaggaatcc gacggcatcc
     3061 agttccgcca ttgcattgcg tggaacaacg gcggcacaaa cggcgtcggc atcgccagcg
     3121 accagtcgag catgagctgg tccaacagcc tgctctgggc caacgccacg ggggcgcagc
     3181 tgacgcaggc cggcacgcat ttcttccgga actccgtcct gtacgccttc ggggccggcc
     3241 ggcgcatctt cgacctcgac accgagacca gcctttcgga cgtggactcg gattacaaca
     3301 acctggtcct gggcagcggg gccaccatcg cggagaagga ccaggtgttc ggcggcagcg
     3361 acctgtacgg cacgctgatc gactggcaga cgcggaccgg gatcgacccg cacagcttga
     3421 gccacgagtc ccagtttgcc gacacggtca acggggactt ccggctgaag agccaggccg
     3481 gccgcttccg cctgaacggc accctgacca acgacacggt cacgtcgccc ctgatcgaca
     3541 cgggcgactc cgtgacgacc tggaccaacg agccggaccc gaacggcagc cgggtgaaca
     3601 tcgggcccga cgggaactgg tcgttcgcca gcttgagccg gaccaacccg tggctgctgg
     3661 ccgtgtccct caacgcggga ggcaccatcc ggggcaccaa caccctgcgc tggacctcgg
     3721 ggaacatcgc cacggacgcc ctcgtccgcc tcgagtactc ccgcaacagc ggcgtcgaat
     3781 ggcggctgat cgccagcaac atcgtcaacg gcgtcacggg ctacacgtgg aatgtttccg
     3841 ccgaaaccac gaccgtccag ggcgcgtggc gcgtgatata ccaggccgac acgaacgtgt
     3901 gggaccgcgc ggacgtcgat ttcatcatca agaacggcac ggtgaacatc tacgtcaacg
     3961 acgacagcac gaacggcgac gtgtactgcc ggggcctcac cgccctgggc aacgatgggc
     4021 agagcgggtt gaccaacacc cagccgatgc ggacgccctc ctacgccatc gcgcggtacg
     4081 ccctggggcc gggcgacact gtgtacatcg acacgggcac gtacaacatg agcagcaacc
     4141 tgtacctcgg cgaactgaac cgggggacgg aaacggagcc catccgcatc ttcggcagca
     4201 ccaactgggc ggccgggggc acgctgctca accgcggcaa cgcgttcgag ccgggcatcc
     4261 ggctccagaa cacgcactac gtggaactgc gcgacctgcg catcatggac gcgctggacg
     4321 gcatcgagat cgacaacgtc atcggcgccg tcctcgagcg gatcgaggtc ttcaagagca
     4381 agaacggcat ttccacgcgg actctccggg acggccggtt ggaccagtgc gtgctctgga
     4441 acaaccggaa ctggggcctg accgcgctct cgcagggcac cctcacgttc cagcggggcc
     4501 tgttctggag caaccgcacc ggggccatcc agctccagag ctcgtcgctg gcgatctcga
     4561 acacgattat ccacgggcag ggcacgagcc agctgtacca gatcagcgag gcggtcccca
     4621 actccgactt caacatcctc tggaaggagg aaaacgcgat cctggttcgc gacacctaca
     4681 acgaggtgac ctaccccaac ctgcagagct ggcagcggaa cttgaacgtg gacgagcaca
     4741 gcgtcctgct caaccccatg ctgttcatgc cgaccaacgg cgacttccac ctgatgagcg
     4801 acgccgtatc cgggcggtac agccgcgcct ccgggcggtg gacgaacgac gcctcgcact
     4861 cgtgggcgat cgacgccggc cacccgtcgg ccccgtatgt caacgagccg aatcccaacg
     4921 ggacgcggat gaacgccggc cacttcggga acacgtggga ggccagccgg agttccacga
     4981 acaaggccct gctggcgacc agtttcaacg acggcggcct cgtgagcggc agcgacgtgc
     5041 tctactggtt gagccgcggg ctgacggcgt cggacctggt gcggattgag ttctcccaga
     5101 acaacggcct ggaatggacg gtcgttgcgt ccaaccggcc ggccaccgag gccgggtacg
     5161 agtgggacac cagcagctat ccctcgacgc cgctggccct gtggcgggtg gtcctgcagg
     5221 ccgacaccaa tgtcgccgac gcggtggacc ggacgttcat cctgcgcaac gggccgatca
     5281 tgttctacgt caacgacgcc tcgaccaacg gagacgtcta cacccggatg ccgggcagcg
     5341 cggcttacga cggcctgtcc acgaacacgc cgatgaactc gctgcagggg atcctgatct
     5401 acgatctgga ggccggcgac accgtctggg tggacacggg ccggtacacc aactatgcgc
     5461 cgatcgagat caacaccctg cacagcggcg ccagcaccgc ccgtgttcac atcgtgggca
     5521 gcaccaacgt gccggccggc ggctcggtgc tgatatggga ttactccgag tgctgcccgg
     5581 ggaacggcat cacgttccgc aacgcgtcat ggatcgaaat gtccgacctg gagatccggg
     5641 acgtggaaac aggcctgcag ttcgagctgt acagcggcaa caacctgatc cgcaatgttc
     5701 agatttacaa cgccgaggaa tacggcgtgt acgtgagctt gagctccggc aaccagttca
     5761 agaacctgct cgtggccggc cgctcgggca aggggctcgg ggtcagttcg tcgccgtctg
     5821 tctacctgga cagctcggtc atctggggca acgaggattc ggcgatcgag gccggcacca
     5881 ccgcgctcta cctgaccaac tcggtgctct tcgcgtcgtc ctcgagcaac tactgcctgc
     5941 ggctgttcac gaacaccacg gtgaccgcgg actacaactg ctacttcagc acgaacgcgg
     6001 ctccgtgcgc cttttataac ggcgtggtct acgacaagct tctccgctgg acggaagcca
     6061 gcgcccagga tgtccactcc ctgaacgagg atcccctgtt cgccaatgcc acgaatttcg
     6121 atttccatcc ccgcagcgtg atcggccgtt accagcgggg aacggggtgg accagcgacg
     6181 ccgaccactc gccgctgatc gacgccggcg cgccgacctg gccgttcgac ctggaatacg
     6241 aacccaacgg cggccggatc aatatcggtc tctacggcaa cacggtcgag gcgagcaaca
     6301 gccggacgaa cgaatggctc cgggcgctga cggggtccag cggcggccgc atcagcgggc
     6361 tcttctccct ggcctgggcc ggctggacct tcgcgccgac caataccgtg cgcctggact
     6421 actcctatga caacggcggg aactggacga cggtcgtcga gcacgtgctg ctgacgaact
     6481 ggtcgtacct gtggaacagc acggacgcgg ccttgagccc gatctgccgg tggcgcgtgg
     6541 tgctggagtc gaacaccaac atctgggaca tcaacgactt cttcttcggc atcaacggcc
     6601 cgttccggtt ctacgtgaac gacactaacc ggaccaacga cgtgtacacg accgcgatcg
     6661 gcagcgacac caacttcggg ctgtatcccc acatcccgaa ggcgacgctg aacaacgtgc
     6721 ttgagaccta cgacctcgag ggcggggacg ccgtgtgggt ggattccggc gtgtacatca
     6781 tcacgaccaa caatcccatt gtctggcccg ccggcgacag cggcagcccc ggctatcctg
     6841 tgtacctgtg gggcaacacg aacgagcccc tgaccatcct gcgcgggcaa tgggcgccaa
     6901 gcccgctcct ggccaacctc acgttccagg ccacggacct gcgggtggcc gggttcaact
     6961 gccaggatgg gcacatgagc gccaccgggt accggctggt gttcgacgac ctggagttca
     7021 gcaacgcgtg gttctacatg atcggcgggg cgcagacggc ccggacggtc cgcttgacgg
     7081 ccggcacggt gtccgtggcg ggcacgggcc tcgggctgca ccggtttgcc gtgcgggaag
     7141 ggaatatcgt cgccggaacc aacgcggtgg agctcatcaa ttccctggtg accggcggca
     7201 gcaacccggc cgtctccttc tcgggcacgg atctgctgct gcgcaacaac acgctggtcg
     7261 gcaccggcac ccagatccgc aaggccggga ccggcaacgt ggtgatcgag aacaacatca
     7321 tctccgcgtc gggcctggac cggttctgca tcctgctgga agacgggacc atggagtcgg
     7381 actacaacaa tctctacgcc gtcagcaacg cctggatcgg gaaccaggac gggtactggg
     7441 agcgcctgat ctattggcag gaggaatccg ggcaggacct gcacagcgta gccaccgacc
     7501 cgtacttcgc cgacgcgacg aacggggact accacctgcg ctcggcggcc ggccggtgga
     7561 ccgacggcgg ctggaccaac gatgccgtgc actctccctg catcgacgcc ggccatcccg
     7621 acagcgtcgc gaccaacgag cacctgccga acggctaccg ggtcaacctg ggcgcctacg
     7681 gcaacacgga ggaggccagc tggagcgtga ccaacgcctg gctgcgggcg ctcacgatga
     7741 acgacggcgg cgtgctccgg ggcaccaaca tcatccgctg gacatccggc tggctcggcg
     7801 ccggcgacct ggtgaggctg gaatactcga cgaacaactt tgcgtcctcg tacacgatcg
     7861 ccgccgggat ttcggccacg gatggccagt acatctggga caccacgctg gtgccgtcca
     7921 gcctgtcggc cgggtggcgc gtggtgctgg aaaccaatac cgcggtggag ggccggaccg
     7981 agcagccttt cgcggtgcgc aactcgccgc tgccgttcta cgtcaacgac gcctccacca
     8041 acaacgacgt gtacacgctc cagccgggga gcgcgtccca cgacggccag tcccagacca
     8101 cccccaagga ttcggtgaag gggatcctgg acgcctacga cacggagccc ggcgacctga
     8161 tctacgtgga caccgggtcg tacacctcgc ccgtggacac ccgcatcata tggtcgcgcg
     8221 gcggcgaagc cctgcgcggt tcggtcctga tccagggcag cacgaactac gccgccggcg
     8281 gctcggtcct gacgcgcagc agcctgccgg ggaccatgct ctttgacgtc aaggcgtcct
     8341 acgtcagcct ccgcgacctg gccatccagg gcgccaaccg cgggatcttc ctcgagtcca
     8401 accggttctg ctccctggaa cggctgatgt tgaggtcgaa caactacggc gtcgtccttc
     8461 acggggccgt cggcacgacg aaccggaacc tgggcttctc gaacaaccgg ctcggcggca
     8521 tggacatcct ggacggccgc acgacgctgg tggagttctg cacgttcgcc ggcaactcca
     8581 acttctcgct ccgggtggac gactcggtgg ccaacacgtt gcagaacaac atctactacc
     8641 tcaatacgat ctcgacgacg aactatgccg gagactccaa cgccctctac agcgctgtta
     8701 tcgactacaa cgtgtatcac ttcgtgacgc agttggtcgt gcgggccgtc accaatatcc
     8761 tgccggtgcc gccggatagc gccatcacca acatcgtcgt gttcaccaac atcgagccac
     8821 tgtgcatcta cgggagctac tcgaacctgc aggcctggca gttggccgag gtgcacgact
     8881 accgcagcag catcacgaac ccgatgctgg cggacgcgga ccgcggcgat ttccatctgc
     8941 ggtccacgac cgggcggtac gtcgcaggct ggggctggac caacgacacc gtgaactcgt
     9001 gggccctgga caaggccaac ccggcctcgt cctggaccaa cgagccgtgg cccaacggcg
     9061 cccgcgccaa cctcggaatg cacggcaaca cggagcaggc cagcaagggc ggcgtcgcca
     9121 cgcagaccct ggtccgcacg ttcaaccagc ccgtgtatgt cagcgagacg aacagcatca
     9181 tgccgcttat ctggcacgtg cggaacgtgc ccaccggcgc ggtcctgaat gtccagtatt
     9241 ccggcgatta cggcaccaac tggatcacga ttctcagcgg ggtgaacgcc tacctggagt
     9301 actacctctg gacgcccaca ccgtactaca acacctacaa ggggaagtgg cgcgtggtag
     9361 gggcgttcga gaccaattac tgggacatga acgacgacat cttcaacatc ttctacggtg
     9421 aatttgcgtt ctccaaggtc cgtcgtgatc cgacgctgac cgaggttacg tggcgcggcg
     9481 cctgggcgga ggaataccag ttgcagtacg ccaccaacac cacgccgacc acgaatatcg
     9541 tggccacctt tatttatacc aacatggtgg gcaccaacgt gctgatccgg acgaatgttg
     9601 tgcgggagat cctcatcaac tggaccaacg ccccgtcggg ttcggggacc aacgagcagg
     9661 cgaatttcgt gtcgccgaac ggcggggacc tgttgtacgt ggatcccacc tccacgggcg
     9721 ccccgccggg ccgactctat cgagtgatcg acaagagaag cgcgccgtga cggtcggccg
     9781 gcgcgccctg gtctggatgg cggtccttgc agcgctgctg gcccccgtgg ggcagggctc
     9841 cgaggaccgc ttgatggccg gcctgcggga cgcgctgcat ttccccttgt tcgccctgct
     9901 gacctggagt tggggcggcc gttttctccc gtgctggcgg acggccgcgg tcatgaccct
     9961 ggcggttctc ggggttgaag ccgtgcaacc gctcgtcggg agggaggcgg aatggcgcga
    10021 cgcgctgttc ggcctggcgg gcgttgtaat ggcgatggcg ctccagggga tgcgtcagcc
    10081 gcggcacgcg actttctgga aagcacttat gatcgttgcg ccggcgattg tgctgtgccc
    10141 attgctgatc gtcggcgcgg accgttggga ggcgcgccgg gccttccccc tcctggcggc
    10201 attccggtcg ccgtgggaga tcggccgctg gacgggacgg ggttgcgacc tccgacgcgt
    10261 gcgccgggac ggggcgtggg cgatgcgcgt ggcggtccgg aatacggccc cggcctatcc
    10321 cggcgccttt ctcatggagg cgccgcggga ttggagcggg atggcggcgc tccaggtggc
    10381 ggtttgctgg cccgggcccg atgagaggga gttgtgggcg cgggccgacg acaggccggg
    10441 ttcgccgccg tacagcgacc ggctgcagaa ggcgctggcc atgcgtccgg ggaccaatcg
    10501 ctgggtgatc tccttcgagg agtggaagag caccccgggc ggccgtcctt tcgacttccg
    10561 gcgcgtgcgg tccctgggcc tgtttttcgc cgaggcggcc ccgggcgaag cgctggatat
    10621 cctggagatc cggctcgttc ccgcgagccg gccccccgcg ggggctgctc aatagtagtt
    10681 gcatccgggt cccgttgaaa tatcatgacc ggaagtgaat catcgcctcg gagccgcggc
    10741 atgaagcttt ccatcatcat tcccgtctac aacgaggaag ccaccgtcga gaaactggtc
    10801 gagttggtgg aatccgtcgc ggtcccgacg gagaaggaac tcgtgatcgt ggacgactgc
    10861 tcccgggacc ggacccgtga aatcctcggg gagttgaaaa tccgccaccc gaacatccgg
    10921 ctggtctttc acgagaaaaa ccaggggaaa ggcgccgccc tgcgggcggg gtttgccgcg
    10981 gccgcggggg acatcctcct gatccaggat gccgacctgg aatacgatcc gcgcgagtac
    11041 ccgcgccttc ttgcgccgat cctggacggg catgccgacg tggtcttcgg atcgcgtttt
    11101 ctcgggggcg gtccgcaccg ggtggtcttc ttctggcact atgtcggcaa caagatcctg
    11161 accacgctgt cgaacatgat gaccaacctg aacctgaccg acatggaggt ctgctacaag
    11221 gtgttccgcc gcgaggtcat gcgggggatc acgatccgcg agaaccggtt cgggttcgag
    11281 ccggagatca cggccaaggt ggcgcggggg cgctggcgta tatacgaggt gccgatttcc
    11341 tactacgggc gcgactattc cgagggcaag aagatcacgt ggaaggacgg gttccgcgcc
    11401 ctgtggtgcg tggtgaagta ccgtttcgcc agctgacggg ggctcatgac cgagccgaac
    11461 aaagtcgaga tcatctgctc ggcctgcggc gcggaggcgc tgctgatgag ggagccccgc
    11521 tacgagggat tccgcaggat cggcgagacg ctgacctgct cggcctgccg acacgagtac
    11581 ccatccgagg aggaggtgcc cttcaaggcg gccgcgcggc ccgtcgtttt ttccgacgcg
    11641 gaccggcccg cgcccgtccg tctgttccag gagggcgagg ccgaccggtt gtgccggcac
    11701 tgcctgcact acgtggtgaa cccgttcatg cagtggtgcg gtgtgcacag gaaagaagtc
    11761 gaggccacgg acacctgttc ccgcttcaaa cgcaagccgg accccaagcc tcccccgccg
    11821 tgacgccggt ttacaggatg ccgccgccgt cgtatggtag agcgcatgaa aggcaggata
    11881 tcccatggct gaactcctga ttcgcaacat tcacaccctg gtgaccatgg acgcgaagcg
    11941 ccgggttctc cgggacgcgg acatccgcct ggaagacggc cgcgtcgcgg agatcgggag
    12001 ccgcctgccg gcccggccgg gcgcgcggga gctggacggg cggaactgcg cggcgtaccc
    12061 gggcttcgtg aatacgcacc accacctcta ccagaccctg acccggaaca tcccgcgggt
    12121 gcagaacgcg aagctgttcg actggctggt caacctctac gaggtctggc gcgaactggg
    12181 cccggaggcg gtcgaggtca gcacgcgcgt cggggtgggg gagctgctgc tctccggctg
    12241 cacgacgacc accgaccatt tctacgtctt cccgcggggg gcgccgcagg agttcctgga
    12301 catcgagatc gagacggcgc gaaagatggg cgtgcgtttc caccccacgc gcggcagcat
    12361 gagccgcggc cgctcccagg gaggcctgcc gcccgacgac gtggtgcaga cgcccgacga
    12421 gatcctggcc gattgcgacc ggttgatctc gaagtaccac gacccgaagc cgttcgcgat
    12481 gtgccggatc gtgctggcgc cctgttcgcc gttctccgtg acgaccgaac tgctggccga
    12541 gacgtcgcag tatgcgcgga ccaagggcgt cttcctgcac acgcacctct gcgagacgaa
    12601 ggacgaggag gatttctgcc agaagaccct gggcctgcgc ccgctggact acatggagaa
    12661 gaccggctgg gtggggccgg acgtctggta cgcccacggg atctatttca gcgaggccga
    12721 gatcaagcgt cttgcggaga cgaggacggg agtggcccac tgcccggcct ccaatctgcg
    12781 gctgggttcg ggtatcgcgc cgatcccgaa gatgctcgaa gccggcgtcc gggtcgggtt
    12841 ggccgtggac ggcagcgcga gcaacgacgc ctcgaacatg gttcgcgaga tgcagttggc
    12901 catgctcgtg caccgcgtcg gcacgggcgt ggaagctatg ccgccgcaga aagtggtgga
    12961 gatggcgacg ctcggcggcg cggccgtcct ggggcaaccg gagatcggaa gcctcgagcc
    13021 cggaaaggcc gcggatctcg cgttgttccg cctggaccgg ctcgattacg cgggcgcgat
    13081 ggcggatccg gcttccgcgc tgcttttctg cggcgccggg ccgcgggccg agtacacgat
    13141 cgtcgccggc agggtcctcg tggaaaaggg gcggcttgtc ggcgaggacg aggaagcgct
    13201 cacggcccgg gccaacgcgg tggcggagcg catgctgcgg gccgcggaag aaaaacgcgg
    13261 cgtgttgtat cgctaggggc cgatggaccc ggacccgccg ggataggtgg agtcgaggta
    13321 ttcctcgctg ttgggaacga gatcgccgtc gggatcatcg tccccctccc agatcagcga
    13381 attgaagtag tacatctccc aggcgtcggg caggccgtcc aggtcatcat cctgcaggta
    13441 caacggcgcg ttcgtgtcgg atgaccaggc cccgtaatag acgttggtgc ccgtcacagc
    13501 gccttcgtcc gagtagcggt acacgtggaa cacgctgcgc acgcgccagt acagggcctg
    13561 cccggtcggc atcgggtccg agaggatgga ccacccgtgg tagctggagt aagcctgggc
    13621 ctcgacgttg gtgacggcaa aatccgggga cgtgctcaac tgccattcgt agccctggct
    13681 ctggtcgatg taggaccagc tgaagaaaac agaggcatag gtggccgttc cgccgttcgg
    13741 atacagggag ctgatgggcg tccacagccg caggaaacgt tcccggagcc aggcctccgc
    13801 gacttccggc acgtaggtgg cgtgctggaa ctcgctggtg aagccgggcc ggttgaccag
    13861 cacgctcgcg gagtagggca ccacgatctg cgcgttgaag gacgtggagg ccgcgtgtgc
    13921 gacgtcctgg gtcagccacg tgatgttgta ggtgaagttg gtgacgccgt tggtgacggt
    13981 cggatacgga taggcctgcg ccatgacata gctgccgaat cccaggttgg tcgacgcgag
    14041 gtctttcacg tactgcagtt gcagccggat ctcgtcctcg gttcgggacg gcgatgtccc
    14101 gtaggaatac gtgcgggtgt tttccatcac gatccagtcc agatggggta tgcgggcaag
    14161 caggcgcagg tcgacgccgg agttcagggc ccagcccacc gagggagaag ccaggtagat
    14221 cgagcccttc catcggtagt tgccggagtt cagttcagcc aactgccgcg tgtacccctc
    14281 catcaggtgg gcgaacacca cgtatcgcca ctcccaccac ttggcccaca ggtcgggatc
    14341 gatgctcaat tccaggcggt tggtcacgga gtcgtccagt gtcacccggc tgtaactcgg
    14401 cgggttggtc tcgtccggcg cgcgcacagg gatggggctg atggggaagc ggatggaggc
    14461 gtatgcgggc ccgtacttgg cggtggtgta ttcccggaac caggcgagcg cgttggagct
    14521 gtacgtgggg ctgtccgtgt agggatcgcc gacccactgg agcgaatcct cgttgaagag
    14581 gaaatacgag tagtccgagt acatcgagag gtcctgtctc caggccgcca ggaagttgct
    14641 caccgtggcg cggtcgccga catcgaacgt cgtggccatg tacttcccgt cgtgcatcca
    14701 gttgaccggc gccaggaccg cccgcccgac atcgccctcc gggatgatct gaatccgttc
    14761 cgcccactgc gggatcgagt tggtgtactt gcggcatatc ttgctgtcga aatggaacca
    14821 gctgctgagc gccacgtcca gtccctcctg gttgcccacc tcgatggtcg tgcggatgtt
    14881 gtcctccagg atgtcccagg tggatggcga ctggtcgccc tccccgagcc aggccatttc
    14941 gcactccacg gcgtcggcca cctgcttgta gcgccggatc aggacggtca tctcctccgg
    15001 gccgatgtag tcgccgatgt ccccggggcg ggtgatgctc tgcaacaggg cctgcgggcg
    15061 accggccgcc ggcgccgtct tgtcgatctc gctgaaggag ggcgtcgggt tggtggagac
    15121 catgccgaag gcgtccgtgt ggagccggcc tcgcacgatc gtggcgttca gcgggtgagc
    15181 gccgctttcc cggctgccga accagacgcg gttggacggg accgccgcgc tccactcgcc
    15241 ccggatgtac gcggcctggt agtacccgtc gccctgctcc cagttgtaca tcgtcaggcg
    15301 gggattgttc acgcgcgtgc ccagggtcag gttcctttcc acgccgttca ggaacagccg
    15361 catctccagc gtgtccgcgc cgctgttgaa gccccgccaa ctggccgtga tgcgattggt
    15421 ctgatgcagc cacaggtgcc cgccgcccag caggtcgcgg tgccggatgg aatgcctcgc
    15481 gccgcccgcg tcgtacatcg tgaactccag cgtgtccctt tcgacgaaca ggtggatttc
    15541 gtgcgacgga ttgggatcgt cgaagaacgc gagatattgc agcgggtcgt tggtcgtcca
    15601 ggcgccgggg acgaatgtga tgtccagcgc gccctccacg gcgggggagg cagtcggcga
    15661 ccgcggatcc aggccggccc gggccgcctc caggttgctg atgccgtcgc cattggcatc
    15721 cgcttccgcc tcgaagaggg cgtggatgtc ctccgtggaa agggccgtcg tgtagatgcg
    15781 aacttcgtcc agccggccct tccacccgga attggtcgag ctcacgtgcc cctggcccag
    15841 ccacacttcc ggtcttgcgg ccgactcgaa acggttactt tgctctccca cgatgcaccc
    15901 gttgatcagc agccgggtca gcccgttatc ctgcaccagg gccacgtgcg tccagcggcc
    15961 ggcataacga tcgggccatt cggcgaggcc cacctcgcgc gaggcgtccg tggaattgcc
    16021 caccaggccg ctgaaccgtc gcgaggtttt atccgcccgc accaggaacc cgggccactt
    16081 gtcgcccacc caccggctgt cggagcacac ggtggcgaaa tccttcggct gcgccgggtc
    16141 gtaccagacc caggcggcga tcgtgaagcc ggggccgccg acgatgggca ggtttgtctg
    16201 gacgatggcc acgtaatcat ccagtccgtc gaacacgagg gccccgcctc gcaccccgtt
    16261 ggtccggctg gagccgggca tgccccgcgc cgtcgcccgc cattccgccg aggccgagtt
    16321 cacgagcgtg gtgccgctgg cctcgtcgaa tttcagccag caggccaact gctcgcgcgc
    16381 ctcgatgccg gacagcgggt tgaacccgta ttgcagctcc catccgtccg gcagcccgtc
    16441 ggagtccgtg tcggcgacgc ggggatcggt gccgtggagg ttgagttcgg cggagtcggt
    16501 caggttgtcc gagtccgtgt cgttgttcat gggatgcgtg cccaggaggt actcctgcag
    16561 gttcgtcagg gtgtcggagt cgggatcgcc gtccgccccg ttcaggcccg cgtcggagag
    16621 gggatccaga cccgtgccgg cctcccaggc gtcgggcagg gtgtcgtcgt ccgtgtccgg
    16681 gtccagcggg ttcgtgtggt ggatcgccgc ctcctgatag tccgacagcc cgtcgtcgtc
    16741 cgtgtccgcg tcccgcgggt cggtccccgc ctggtactcc tgcaggttcg tcaacccgtc
    16801 ggagtccgcg tccgtcgagc cgttggccac cagcggattg aagtactggt tcacttccca
    16861 cccgtccggc atcccgtcgg catccgtgtc cgcctgctgc ggctccgtcc cgcgcccgta
    16921 ttcctgcagg ttggtcagcc cgtcgctgtc cgggtcgtcc gccgcgtcgt gtgcgttcgc
    16981 attcgtcccg tgcccgattt cccagccgtc cggcagcccg tcgctgtccg tgtccgcttg
    17041 cagcgggttc gtcaggtgca ccgtcagctc atcgccgtcc gacagcccgt cgtcgtccgt
    17101 gtccgcgtcc cgcggatcgg tccccgcctg gtactcctgc aggttcgtca acccgtcggt
    17161 gtccgggtcc aggctcgggt cgttcgagag gggattctgc tggttcgcca gctcccagtc
    17221 gtccggcatc ccgtcgccgt cgctgtccgc ctgcagcgga tcggttc
//
LOCUS       DAOOTK010000059        17139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2290_length_17139_cov_4.610220, whole genome shotgun sequence.
ACCESSION   DAOOTK010000059 DAOOTK010000000
VERSION     DAOOTK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17139
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_17139_cov_4.610220"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PK634_08045"
     CDS             complement(<1..699)
                     /locus_tag="PK634_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97723.1"
                     /translation="MARSRLTRWPGWFRAAADGPAAAPLTAALAALVLAVGAAWFSGI
                     PVPRVHDEFSYLLAADTFAHGRLTNPTHPCWKHFDTMHVLQRPTYQSMYPPAQGLILA
                     LGQRLGHPVIGVWMSVAAMAAAFCWMLRGWLPGRWAWLGALLAVAQIVALGREYDHGV
                     IGYWSQSYWGGAVAATGGALVYGALPRLQRRRLARDGILFGLGLAVLAASRPYEGLVA
                     ALPACALLGLDLARG"
     gene            complement(689..2092)
                     /locus_tag="PK634_08050"
     CDS             complement(689..2092)
                     /locus_tag="PK634_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOW97724.1"
                     /translation="MSGPRHGILERIGLLAGAHAFREILQAVFLIVLARRQMITYGQF
                     MLAMGFGQILLMFAEFGLNKHTICRLAHRPDTETGLVWQVTAVKSLLLALGGGATFLF
                     VLVQSYGLSLGTVILLLSAGVGFDALTSTFYALYQWRGDQRREAAVRSVAAAAGFGYG
                     LAALALGLPPAALALYKLVESLAALAGAMLPILRSSQARPLLPGPSAIGATLRDGLVF
                     ALIELAAILYNKANLFFLQRAAGPGGVAQYSATWQIVDGATNLVCGLLLARTLFPLFA
                     RLLGTDFAAARDLARDTARWLLPVAACLMYGLAIESDRLIPLLYGGEYADAAWMQRWL
                     IISVAIGLFHNLAAYLMLSLGRQALLLGICAAGLVLNIALCALWIPGRPLAGSIAALL
                     ATKGAVALCTLGYAQRRLGLLRLRPLAETGAAALAGAVLLFVLRPLGVRLVAESAALL
                     PMLALMLRWKRRTGHGA"
     gene            complement(2089..3330)
                     /locus_tag="PK634_08055"
     CDS             complement(2089..3330)
                     /locus_tag="PK634_08055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW97725.1"
                     /translation="MANTAKAPGLACILRWFPAPSETFIFDEMTALQRAGAPLHLYTL
                     YGCWRGPLSPDMPADDLPITRLGTRALGRMARAIGFWLKTNPPAARRLWRTVPWRRWR
                     DLEMAGENLWAFLCGFYIARLVRNGGFSHLHAPWANGPASAAWVASQLTGIPFSFSTW
                     AGDIFPPDGALSEKIRDAAFVRSTCRSFVPHLRQQAGLHADKIRVLYNGQDPARFQPA
                     EARFQSPLKLLALGRFVRKKGFHVALHAAARLKAEGVSFRLTVGGDGPENSRLRRLAR
                     SLGLTGDVSFPGFVPRQRTSDLFRDCDVFLMPSVVDPAGDRDGIPNVVLEALLHRIPV
                     VATNIGGIAEVIRDGVTGCLVPPNDPESLAAAVRRLAGGPDAARAMAERGRRFVLQEF
                     HLDTNARRLFELIAQHSGPTP"
     gene            3471..4703
                     /locus_tag="PK634_08060"
     CDS             3471..4703
                     /locus_tag="PK634_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOW97726.1"
                     /translation="MLPRSRFQAFVDMVEQGKEGGYYPFFRPLAESWGPEVDVGGRRL
                     IMVGSNDYLGLSHDPRVVAASQEAARRWGAGPGGSRFLCGNTTLHEALEERLAAFIGK
                     KKAVVHTTGFTTNSGALHCLAATQDVLVCDSESHASIVDGCLFSRGRLLTFRHNQAES
                     ARAKIEAAQEKSPEACFLVTEGVFSMSGDVAPLSELLPLKQRFPSLLVYLDDAHGLGV
                     MGRGGRGTADHFNATAGVDFIMGTFSKALASIGGFIASDDTDALTYIRHQSRPLIFSA
                     ALPASCVAAALAGLDVLEREPERVERLHRNTRLAWEGYRRIGLATRYSGSPILPITVG
                     SEMKAYQLSRELFELGVFALPAVFPAVPRGQAIIRTAYMSTHEPRHIEQVLSVLESLA
                     ERHGLRGVVPEPEPIAAG"
     gene            4978..6165
                     /locus_tag="PK634_08065"
     CDS             4978..6165
                     /locus_tag="PK634_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW97727.1"
                     /translation="MLDFHDLLRVSFRQVRRQRSRYLGVLLTVTLGTSGFIAVNTIGR
                     NVKSNLDRDLDVLGGVTIVKVFFDDEAAGRPPTKFRPATLDALRAIPGVEGISAMAMK
                     MVNARVVLRGERYGFTLVGVDEHFWSVNRFAAVEGRLFSAGEIDSRRTVCVLGAGLAR
                     QLYGTGPAVGQDLLVDGDLYRVTGLLDGPGVGDRDQWAFIPVTTAQDRIHPLIPPNRL
                     YVRCRSVEAVRSVTAAIPDAIARVQPADHLRVEVGWERLKHVQRMVWWVQAFVHIAVA
                     ATLALGGFGIWTIMMSAARSRTREIGLKKAMGAEDRDILAQFLAESATLSLAAGLLGT
                     ALGWLAVRGIGAWLGSRPPPETLWLYMGVSLFVAFALGVGAGFYPSFHASRMEPVTAI
                     KYE"
     gene            6158..6853
                     /locus_tag="PK634_08070"
     CDS             6158..6853
                     /locus_tag="PK634_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW97728.1"
                     /translation="MNDEASRNGQLLQVRGLARRYDTAAESLHVLKQVDLDVRAGESI
                     AIVGPSGSGKSTLLFILGLLLPPSSGSYRMEGRDMLALDRRTQADLRRRAFGFVFQSC
                     NLIEHTTVRENLEFPLMYAGVPRGEREARIREALDAVDMAHRIRHPTNLLSGGEQQRV
                     AVARALVNRPRVILADEPTGQLDRAHGRQIMDHFHRILSGGDKAMIVVTHDPSVAECC
                     ARVCMLEDGVLKG"
     gene            6886..7419
                     /locus_tag="PK634_08075"
                     /pseudo
     CDS             6886..7419
                     /locus_tag="PK634_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
     gene            8019..9269
                     /locus_tag="PK634_08080"
     CDS             8019..9269
                     /locus_tag="PK634_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW97729.1"
                     /translation="MKYRLVMIGVLLAGGAVARAGEPPLTFRDGVARALEHSPYLAVG
                     ELDVAVQRLGEADARSDFIPQFSLRTRYYLDPPAGSDERFQLSFTVDPYNPVEAKLSV
                     EARELLTDLAVLSHLQAVAECLHRVGQGWLELEALSELEAVERAGLELAEAAAAASSD
                     RAARGVGTEPDAQAAGRELEIRRLRLDHLKRTRADVLEGLGALIGASPAEPLNVDAKD
                     AGAEVLGDPPGAETAWEAVRDRSIEAQARELRRKLQEIRILAARAAYIPDLWLGVSTP
                     DPLSGFNDGDFYFSVGVDIPIWDGWKRARDIARQKQELRRQDQSGLLDILDFRARWRA
                     AESACRAAEIEWRAAQAAEERTALRRRQAGEGEAERAARADWLEARRRAIQSGWAWRK
                     ACLDRDALSGALIDRLCKTETEQP"
     gene            9266..10150
                     /locus_tag="PK634_08085"
     CDS             9266..10150
                     /locus_tag="PK634_08085"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW97730.1"
                     /translation="MKAILGALVLAALPAMAAPEETAVRGELFCSVERRVVLPYPGTI
                     TAIAAEVGQDVKRGDPLLQFTLSRRARAELEPLASQASQAAAEAEVAGLEAEAGEARA
                     AAEEAERLAREQLGTRPQADLAKSRVAALDKRLTAARERAEYERRQRDTNLRWLKEGL
                     GIENPENGIPETVPLPAPMDGTVIWISGDARPGVELDPSTPLLTLGVMDPMIVRAHVH
                     ELDVVKLKVGDRAHMTLLARPDAKLEGAIRRIAWAPFPHWLPEPSYFEVELTVPNPDR
                     ALRQGYKVNLQFGDAAAE"
     gene            10154..11305
                     /locus_tag="PK634_08090"
     CDS             10154..11305
                     /locus_tag="PK634_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97731.1"
                     /translation="MSGPAVEILPVRTAREREAFIRFPWQVQAADPCWVPPLVSRQRE
                     FLDPRRGPFFEFGEAECFLARRGGRWAGRISAQVSRRHDEFHGAGTGFFGFFECEDDP
                     EAAGALLRAAADWVRARGRQRLVGPMSFSIYDDIGLLVDGFDSVPALLLTHNPPWYAE
                     LVAAQGFRKAVDWHALRVTNTDIDPAAMQTEAAEIIKRSGLVLRMPSPAEMVRRSDEI
                     LELFNEAWDGNWGHVPFTSLQFKVILGELRPLLRTDLIRLVLDGGRIVAFTITIPDLN
                     PLIRRFDGRLSLWNMLRLYRAAQWAPLKRVKTVLLGVKPVFRNRRLHDAMILDTYAHL
                     VRTQPGIEQCDCSLICEQLRVFIRSLERYGARPYKTYRIYEKEIWSKAR"
     gene            complement(11323..11679)
                     /locus_tag="PK634_08095"
     CDS             complement(11323..11679)
                     /locus_tag="PK634_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97732.1"
                     /translation="MSLRASIFATCLLCAAASGAESPVTGIWRGQVLFFRITVQAAQW
                     DSQAMGVAEVRGPLGGQYTYHFNGLVESNRLHGVHHSGHRFEGERTAEDRISGLLTTR
                     KGRRFRLELRREEPAP"
     gene            complement(11676..12923)
                     /locus_tag="PK634_08100"
     CDS             complement(11676..12923)
                     /locus_tag="PK634_08100"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HOW97733.1"
                     /translation="MNAPRPGRLAGILLLLAGVQVLVMALVSPLNAHPDEELHVTTAR
                     FFLDRWLPPAADDPRVPGHLDPVYGTSYLLASPPQILYPLAAKSARLFGFSNRWALGL
                     RLWSVAAFMVLAAAAWRLAPRTLLPAVCLLWTPQVWYVFSYFNSDAFPFFLSFLIALL
                     VGWPGPTDWRRSAPAALLLGLLLLAKHNYWPFAAFAVLFLALRSGPKRALAVAGLTAM
                     VAAPLLVRDAVINRFEKEERKAALREEYAADAFKPSRLGTPGADPGLALRAQGVSLAD
                     MLTQRPWARRITRSFGGAYGHMDILAPPAYYIIQAVLFTGLLLAAAIAWRRGWAPARL
                     ALLLLAVLAGAAVILIAALFCWMYDFQPQGRYLFPLLPIFLLAGLGLSGDDPRRIPWP
                     VPLALFLLGSYSFALVGLPRLWP"
     gene            complement(12920..13483)
                     /locus_tag="PK634_08105"
     CDS             complement(12920..13483)
                     /locus_tag="PK634_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOW97734.1"
                     /translation="MFGYSDILWEFRLVCRHTRWAPLRAVLVWIYDALQFLGGGWISH
                     EAEFGGKPCLPHGIRGVFVSRHARVGKNSVIFQQVTIGSNVLPDSSGLGAPVVGDECY
                     IGAGAKIVGRVTVGARSRVGANAVVRESVPENGIAIAGAPVGVAREPPDNRFFTRRKG
                     RWFFYRDGQWLDERDPAILEKLNGAFR"
     gene            complement(13494..14822)
                     /locus_tag="PK634_08110"
     CDS             complement(13494..14822)
                     /locus_tag="PK634_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97735.1"
                     /translation="MITDFNLRTFFHPWRIVRHRRFLARAERWPADRLKSWQDARVRE
                     LVRYAYEQVPYYRELFDREGLKPGDIRGAADLPRIPTLTKDIVRERAADLRPREFDTL
                     RPVPVRTSGSTGTPLQFYADRDLAIAKFATFWRAWNRAGYRIGQRWALIAGPVFDDGA
                     LFRRVRSMNALYVSSFNLTAETARRILGELLRFKPVFLRGYPSALYEFSRFIGDPAAL
                     RRLGIRSISTNSENLLPFQREQIEAAFGCRVFDVYSQWEQACVIAQCERGSYHHEAEY
                     GILEILDPLGRSAPPGETGEIAGTNLFNRAMPLIRYRTRDLARISPKSCPCGRAHTVV
                     EAIDGRIEDVVVTPDGRRVGRLDAAFKWNAGFDFAQVVQDAPGGIVVRLVRNEAFRPE
                     ILADLEKHLRDRVGAALGIRFEFPDRIAPDANGKIRFVINKQLHSPAPVP"
     gene            complement(14819..16015)
                     /locus_tag="PK634_08115"
     CDS             complement(14819..16015)
                     /locus_tag="PK634_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOW97736.1"
                     /translation="MKPEPATDKTIFILGAGIYQAPLIRQARIMGLRVLVASRPGPYP
                     GFALADRAFHVDTTDAAAVLGVARAEKIDGICTSGTDVALAALGGVVDALGVPGPSAA
                     ATAIATNKLLMRRAGRQAGVRMPEFRAARTAAEALTAARELGTPVMVKAVDSSGSRGV
                     VKVRGTGEIDSAVAKVRAVTRREEFLVEQFIEGADCGAEAFVLNGRVVYLLPTGCTVF
                     EGEAHVPIGHHLPHSEGLARLAEVRDQVERCVRASRLDHCAMNFDFIVNEAGVHVIEF
                     GARAGATCLPELAGIYGGFNYYEQILRAALGGTPEFPAGTGTPCAAVLLRADRDGVIR
                     EQRNENGPDPEIVDVSFDYRPGDRVRRFRVGPDRIGHIIAKGARPGDALAALDRALRG
                     LRIVIE"
     gene            complement(15990..>17139)
                     /locus_tag="PK634_08120"
     CDS             complement(15990..>17139)
                     /locus_tag="PK634_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOW97737.1"
                     /translation="RIARPPALAAARAQTLLRPSFLGPNLEAYLDMVRRHGLRLTWAA
                     LRGGLRAQGAGFRLLPVLRQALFWLLPVLILAVIFRRIDWPMFRENFTRAAPGLVLLG
                     LLYYPLVILVGALRWRLLLEQFHRERVGYGFALKHYWIGLALGLFGPASIGWDAYRLV
                     AAGRHFGRYAPGAAAIVTEKLLALVTCAVLVVGLYPLLPIEAGPAARAVLTTACALLG
                     AAGLLFLFLLLARRRLDRFVSALAARLLRRAPGTEPPSLIEPVTSWRLLRGTLGLSLA
                     NLLIAALGNQIFFRAVGWPIPYLANLFVLPVLMFVFLLPISFGSLGIREGAYILLYGL
                     FGVPPETALLVSFFNLAGLLFNTSIGVILMAAQGWMRPRTHETRTGNG"
BASE COUNT         2834 a         5760 c         6121 g         2424 t
ORIGIN      
        1 cccgcgcgcg agatcgaggc cgagcagggc gcaggccggc agcgcggcga cgagtccctc
       61 gtagggccgg gacgcagcga gcacggcgag gccgagtcca aacagaatgc catcacgggc
      121 gagtcggcgt cgctgcaggc ggggcagggc gccataaacc agcgcgccgc cggtcgcggc
      181 cacggcgcca ccccagtacg actggctcca gtagccgatc acgccgtggt cgtattcgcg
      241 gcccagcgcc acgatctgcg cgacggccag cagggcgccg agccatgccc accggccggg
      301 cagccagccg cggagcatcc agcagaacgc cgcggccatg gcggcgacgc tcatccagac
      361 tccgatgacc gggtgcccga gccgctgtcc gagggccagg ataagcccct gcgccggcgg
      421 gtacatggac tggtacgtcg ggcgctggag cacgtgcatc gtgtcgaagt gcttccagca
      481 cggatgcgtg ggattcgtca gccggccgtg cgcgaaggtg tccgcggcca gcaggtagct
      541 gaactcgtcg tgcaccctgg gaacgggaat gccgctgaac cacgcggcgc cgacggccag
      601 gaccagggcg gcgagcgcgg ccgtcagcgg ggctgccgcg gggccgtcgg cggcggcccg
      661 gaaccagccg ggccatctcg tgagccggct acgcgccatg cccggtcctc cgcttccacc
      721 gcagcatcag cgccagcatc gggagcagcg cggccgattc cgccacgagc cggaccccga
      781 gcggccgcag gacgaacagg agaacggcgc cggccagcgc cgccgccccg gtttccgcca
      841 gaggacggag gcgcagcagg ccgaggcgtc gctgggcata acccagcgtg cacagggcca
      901 ccgcgccctt ggtcgcgagc agcgccgcga tactgcccgc gagaggccgg ccggggatcc
      961 agagcgcgca cagcgcgatg ttgagaacga ggccggcggc gcagatgccc agcagcagcg
     1021 cctgccgccc caggctcaac atcaggtagg cggccaggtt gtgaaacagg ccgatggcca
     1081 cgctgatgat gagccagcgc tgcatccatg ccgcgtcggc gtactctccg ccgtagagca
     1141 gggggatcag gcggtcgctc tcgatggcca gcccgtacat caggcaggcg gcgaccggca
     1201 gcagccagcg ggccgtgtcg cgggccaggt cccgcgcggc cgcgaagtcg gtgccgagca
     1261 ggcgggcaaa gagggggaac agcgtgcggg cgagcaggag cccgcacacc aggttggtcg
     1321 cgccgtccac gatctgccac gtggcgctgt actgggccac gccgccggga ccggcggcgc
     1381 gctggaggaa aaagaggttg gccttgttgt agagaatcgc ggccagctcg atcagcgcga
     1441 agaccagccc gtcgcgcagg gtcgcgccaa tggcggacgg gccggggagg agcggccgcg
     1501 cctggctgct gcggagaatg ggaagcatgg cgccggccag ggccgcgagc gactcgacca
     1561 gcttgtacag ggccagggcg gcgggaggaa gtccgagcgc gagcgccgcc aacccgtagc
     1621 cgaatccggc ggcggcggcc acgctccgga ccgcggcctc gcggcgctga tccccgcgcc
     1681 actggtacag ggcgtagaac gtgctggtca gcgcgtcgaa gccgacgccg gccgacagca
     1741 gcaggatgac cgtgccgagc gaaagcccgt acgactgcac aaggacgaag agaaaggtcg
     1801 cgccgccccc gagcgccagg agcaggctct tgaccgcggt cacctgccag accaggcccg
     1861 tctccgtatc cggacggtgc gcgagccggc agatggtgtg cttgttgagc ccgaactcgg
     1921 cgaacatcag gaggatctgg ccgaatccca tcgccagcat gaactggccg tacgtgatca
     1981 tctggcgtcg cgccaggacg atcaggaaga ccgcctggag gatctcccgg aacgcatggg
     2041 cgcccgcgag aaggccgatc ctttcgagga tgccgtggcg gggcccgctc atggggttgg
     2101 ccccgagtgt tgcgcgatca gctcgaaaag ccgccgggca ttggtgtcga gatggaactc
     2161 ctgcagaacg aaccggcggc cgcgctccgc catggcgcgg gccgcgtccg gcccgcccgc
     2221 cagccggcgc acggcggcgg cgagggattc gggatcgttg ggcggaacca ggcagcccgt
     2281 gacaccgtcc cggatgacct cggcgatgcc gccgatgttg gtggcgacga cggggatcct
     2341 gtgcaggagg gcttcgagta ccacgttggg aatgccatcc cggtcgccgg ccggatccac
     2401 aacgctgggc atgaggaaca cgtcgcaatc ccggaacagg tcgcttgtcc gctggcgcgg
     2461 cacgaagcca gggaaagaga catccccggt caagcccagt gagcgcgcca gccgtcgaag
     2521 gcggctgttt tccggtccgt cgcctcccac ggtcagtcgg aacgacacgc cctctgcttt
     2581 caaccgcgcc gccgcatgca gcgccacgtg gaatcccttt ttgcgcacga atcggccgag
     2641 cgccagcagc ttcagggggg actggaatcg cgcctccgcg ggctggaagc gggccggatc
     2701 ctgaccgttg tacagaacgc ggattttgtc cgcgtggagg ccggcctgtt gccggaggtg
     2761 tggcacgaaa ctgcggcagg tgctccgcac aaaggccgca tcccggattt tttcggacag
     2821 cgcgccgtcg ggcggaaaaa tgtcgccggc ccacgtggag aagctgaaag ggataccggt
     2881 caactgcgag gcgacccagg ccgccgaggc cggcccgttg gcccaggggg cgtgcaggtg
     2941 cgagaagcct ccattccgga ccagccgcgc gatataaaag ccgcacagga aagcccatag
     3001 gttctcgccg gccatttcca ggtcgcgcca gcgtcgccac ggcaccgtgc gccagagccg
     3061 gcgcgctgcg ggcggattgg ttttcagcca gaacccgatg gccctggcca tccggcccag
     3121 ggcccgcgtg ccgagccggg tgatcggcag gtcgtccgcg ggcatgtccg gcgacagggg
     3181 gccgcgccag catccataca gcgtgtagag atggagcggg gcgcccgcgc gctgcagcgc
     3241 cgtcatttca tcgaatatga aggtttccga aggcgcgggg aaccagcgga ggatgcaggc
     3301 caggccgggc gccttcgccg tatttgccat gttttccgcc atgaagacac gctgtaaggc
     3361 gcgaatcgtt ttaacttgga aatgttacca gcaaacggct atgtttgaaa cagcaaggtg
     3421 cgttcggccg cttacagagg ccgacaccgc aggcggggag actgattttc atgctgccga
     3481 ggagccgttt ccaggcgttt gtagacatgg tcgagcaggg caaggagggg ggctactatc
     3541 ccttcttccg gccattggcc gaatcctggg ggcccgaggt ggatgtcggc ggccggcggc
     3601 tgatcatggt gggctccaac gattacctcg gcctgtccca cgacccccgc gtggtcgccg
     3661 cgtcacagga ggcggcgcgg cggtggggcg ccgggccggg cggatcgcgg ttcctctgcg
     3721 gcaatacgac cctccacgaa gcgttggaag agcggctggc cgccttcatc ggcaagaaga
     3781 aggccgtggt gcacaccacc ggcttcacaa ccaattccgg cgcgctgcac tgcctcgccg
     3841 ccacccagga cgtgctggtc tgcgattcgg agagccacgc cagtatcgtg gacggctgcc
     3901 tcttttcccg cggccggctg ctgaccttcc ggcataacca ggccgaatcc gcccgcgcca
     3961 agatcgaggc cgcgcaggag aagagccccg aggcctgctt cctggtcacc gagggcgtct
     4021 tcagcatgtc gggcgacgtc gccccgctct ccgaactgct gccgctgaaa caacgcttcc
     4081 ccagcctcct cgtctatctt gacgacgccc acggcctcgg ggtcatgggg cgcggcgggc
     4141 ggggcacggc cgaccatttc aacgccaccg ccggcgtgga tttcatcatg ggcacgttca
     4201 gcaaggccct ggcctccatc ggcgggttca tcgcctcgga cgacaccgac gcgttgacgt
     4261 acatccggca ccagtcgcgg ccgctgatct tttccgcggc gctgccggcc tcctgcgttg
     4321 cggccgcgct cgccggcctg gacgttctcg agcgcgagcc ggagcgggtc gagcgcctcc
     4381 atcgcaacac gcggctggcc tgggaaggct accgccgcat cggcctggcg acgcgctact
     4441 ccggttcgcc catcctgccc atcaccgtgg gctcggaaat gaaggcctac cagctctcgc
     4501 gcgagttgtt cgaactcggc gtcttcgcgc tgccggctgt gttcccggcg gtcccgcgcg
     4561 gccaggcgat catccgcacg gcctacatga gcacccacga gccccggcac atcgagcagg
     4621 tgctgtccgt gctcgaatcc ctggccgagc ggcacggcct gcgcggcgtc gtccccgagc
     4681 ccgaaccgat cgccgccggg tgaacaagtt attgagtact attcttgtga tcttacactg
     4741 atccgcgggg ggtgttacaa cgcgcctccg tgtagcggat agcatagcag tatttccccg
     4801 gcggtagaag gaaatatcca ttaccgggcg cggctcggcg cggctgttga ttgtggctct
     4861 tgcgccctgc ggggcgcagg cgcgagggaa gcgttgacgt ccgcgggccg ggcgggcgaa
     4921 cattccaggt tgagccggag gccccggtcg ggcagaatgc cgttccacgg ggcgaacatg
     4981 ctcgattttc acgacctgtt gcgcgtcagt ttccgccagg tgcggcggca gcggtcccgc
     5041 tacctgggcg tgctgctgac cgttacgctt ggcacgtccg gcttcatcgc ggtcaatacc
     5101 atcggccgca acgtcaagag caacctcgac cgcgacctgg acgtgctcgg cggcgtgacc
     5161 atcgtgaagg tttttttcga cgacgaggcc gccggccgcc cgcccacgaa gttccggccc
     5221 gccacgctcg acgccctgcg cgcgatcccc ggcgtcgaag ggatcagcgc gatggccatg
     5281 aaaatggtca acgcccgcgt ggtcctgcgc ggcgagcgct acggcttcac cctcgtcggc
     5341 gtggacgagc acttctggtc ggtcaaccgc ttcgccgccg tggagggccg cctgttctcc
     5401 gccggggaaa tcgacagccg ccgcaccgtc tgcgtgctcg gggccgggct ggcccgtcag
     5461 ttgtatggaa cgggcccggc cgtggggcag gaccttctcg tggacgggga cctctaccgc
     5521 gtcaccggcc tgctggacgg cccgggggtc ggcgaccgcg accaatgggc gttcatcccg
     5581 gtcaccacgg cgcaggaccg catccacccg ctgatcccgc ctaatcggct gtatgtccgc
     5641 tgtcggtcgg tggaagcggt ccggtccgtg acggcggcca tcccggatgc gatcgcgcgc
     5701 gtccagccgg cggaccacct tcgcgtcgag gtcgggtggg agcggctcaa gcacgtccag
     5761 cgcatggtct ggtgggtgca ggccttcgtg catatcgcgg tcgcggccac actcgcgctc
     5821 ggcggattcg gcatttggac gatcatgatg tccgccgcgc gctcccgcac gcgcgagatc
     5881 ggcctgaaaa aggccatggg cgccgaggac cgcgacatcc tcgcgcagtt cctggccgaa
     5941 tccgcgacct tgagcctggc cgcgggcttg ctggggacgg cgctgggctg gctggccgtc
     6001 cgggggatcg gcgcgtggct cgggtcgcgc ccgccgccgg agaccctctg gctgtatatg
     6061 ggcgtcagcc tgttcgtggc cttcgcgctg ggcgtcggcg cgggcttcta cccgtcgttt
     6121 cacgcgagcc gcatggagcc ggtgacggcg atcaagtatg aatgacgaag cgtccaggaa
     6181 cggacagctg ctccaggtcc gcgggctggc ccggcgctac gacacggcgg ccgagagcct
     6241 gcacgtcctc aagcaggtgg acctcgacgt ccgcgcgggg gagagcatcg cgatcgtcgg
     6301 cccgtcgggc tccggcaagt ccacgctgct gttcatcctc ggcctgctcc tgccgccgag
     6361 ctccgggagc taccggatgg agggccggga catgctcgcg ctcgaccgcc ggacccaggc
     6421 ggacctgcgg cggcgcgcgt tcgggttcgt gttccagagc tgcaacctga tcgagcacac
     6481 cacggtccgg gagaacctgg agtttccgct catgtacgcg ggcgtgcctc gcggcgagcg
     6541 cgaggcccgc atccgcgagg cgctggacgc cgtggacatg gcgcaccgga tccggcaccc
     6601 gaccaacctg ctctccggcg gcgagcaaca gcgcgttgcc gtcgcccggg cgctggtcaa
     6661 ccgcccgcgc gtgatcctcg ccgacgagcc gaccggccag ctcgaccgcg cccacgggcg
     6721 gcagatcatg gaccatttcc atcgcatcct ctccggcggc gacaaggcga tgatcgtcgt
     6781 cacgcacgac ccgtccgtcg cggaatgctg cgcgcgggtg tgcatgttgg aagacggggt
     6841 gctgaaggga tgaagcaaat gcaacatagg tcttataaga ccaatatgac ctatctcatc
     6901 ccgcctcacg gcggataccg ggaactcaag tcctttcaga tgtccgagct ggtctatgac
     6961 gggacggtgg ccttctgcgg ccgattcatt gaccgtcgtt cccgaaccca cgaccagatg
     7021 gtgcaggccg cccgcagcgg caagcaaaac atcaccgagg ggtgcatggc ctccggcacg
     7081 tcgaagaaga tggaactcaa gctcatcggc gtggcccggg ccagcctcga agagttgctc
     7141 caggacctgg aggatttcct gcgccagcgc ggcctgccga aatggggcaa agacgatccg
     7201 atggccaaaa agatccgccg cctggcttat gaagtgaata ggtcctatac gacgtataag
     7261 acctatatcg agaactctcc ccccgaggtg gccgccaaca ctttgctttg ccttgttcac
     7321 caaaccaatt ttctcctgga ccagcaactc cgccggctgg agcgcgattt cctggagcag
     7381 ggtggcttca cggaaaggtt gtatcgcgtt cgaaaccagg tccgggagga taggtccgat
     7441 aagacctata cgacctattc ctactggggc cagcacgaca tcctgcagca gcccttgacc
     7501 gcgttcttct gttctcatcg ttgtcccggg cccctggtgg ccaaggcgta cgacctggcg
     7561 cagaagtggc ggttggaggg ccgtccactg atcggaggct ttcattcgcc tgtcgagcag
     7621 gaaatcctac ggaacatgct ccggtcgcag gcgccggtct gcgtggtcct ggcgcggagc
     7681 ctgccgaaac gaatcctggc ggaatggcgc cgcccgctcg atgagcatcg cttgctgttg
     7741 ctctcgcctt tcgatgccag ggtgaagcgc gccacccggg agaccgccgc gcgccgcaat
     7801 caagtcgtcg ccgtgctggc caagacgata ttcgtcgccc acgcggagcc cgggagcgcc
     7861 accgaggcct tcggccggga aatggcggcc gcaggaaaaa cctgcatgac gtttgacgat
     7921 ccccggacgg agaacctgaa ggccgccgga ttcaaaacgc ttcttctttg aactgtacag
     7981 gaatatgcgg ttaacatgaa ttcatggtgc ggcccgggat gaaatacagg ctggtcatga
     8041 tcggggttct gctggccgga ggcgcggtcg cgcgcgcggg tgagcctccc ttgacctttc
     8101 gggacggcgt ggcgcgcgcg ctggagcatt ccccgtacct ggccgtgggc gaactggacg
     8161 tggccgtgca gcgcctgggc gaggcggacg cccggtcgga tttcatcccg cagttctcgc
     8221 tccggacccg ctactacctg gatccgccgg ccgggtccga cgagcgattc cagctctcct
     8281 tcaccgtcga tccctacaat cccgtcgagg cgaagctgtc cgtcgaggcg cgggaactgc
     8341 tgaccgacct ggcggtgctg tcgcacctgc aggccgtcgc ggaatgcctg caccgggtgg
     8401 ggcagggctg gttggaactc gaggccctgt ccgaactgga ggcggtggaa cgggcagggc
     8461 tggaactggc cgaggcggcg gccgccgcat cctcggaccg cgcggcgcgc ggcgtgggga
     8521 ccgagcccga cgcgcaggcg gccggccggg aactggaaat ccgccggttg cggctcgatc
     8581 acttgaaacg cacgcgggcg gacgtgctgg aggggctggg cgcgctgatc ggcgcttcgc
     8641 cggcggagcc gctcaacgtg gatgcgaagg atgccggggc ggaggtgctg ggcgacccgc
     8701 cgggcgcgga gacagcctgg gaagccgtcc gcgatcgctc catcgaggcg caagcccgcg
     8761 aactccggag aaagctgcag gagatccgga tcctggccgc gcgcgccgcg tacatccccg
     8821 acctctggct cggcgtcagc acgcccgatc cgctttccgg cttcaacgac ggcgatttct
     8881 acttttccgt cggcgtggac atcccgatct gggacggctg gaagcgggcc cgcgatatcg
     8941 cacggcagaa acaggagctt cgccgccagg accagtcggg cctcctggac atcctcgatt
     9001 tccgggcgcg ctggcgcgcg gcggaaagcg cctgccgcgc cgcggagatc gagtggcgcg
     9061 cggcgcaggc ggccgaggag cgcaccgcgc ttcggcgccg ccaggccggc gagggcgaag
     9121 cggaacgcgc ggcccgggcc gactggctcg aggcccggcg gcgggccatc cagtcgggat
     9181 gggcctggcg caaggcctgc ctggaccggg atgccctgtc cggcgcgctg attgaccggc
     9241 tctgcaaaac cgaaacggaa cagccatgaa agcaatactc ggtgccctgg tgctggccgc
     9301 cctcccggcg atggcggcgc cggaagaaac cgccgtccgc ggagagttgt tctgctcggt
     9361 ggagcggcgc gtcgtcctgc cgtacccggg caccatcacg gccatcgcgg cggaggtggg
     9421 ccaggacgtg aagcgcggcg acccgctcct gcagttcacg ctgtcgcggc gcgcgcgcgc
     9481 ggaactggag ccgctcgcgt cgcaggcgtc gcaggcggcc gccgaggccg aggtcgccgg
     9541 cttggaggcc gaagccggcg aggcccgggc cgcggcggag gaggccgaac gtctggcgcg
     9601 ggagcagctt gggacccggc cgcaggctga cctggcgaag agccgggttg cggcgctcga
     9661 caagcggctg acggccgccc gggagcgcgc cgagtacgag cggagacagc gagacaccaa
     9721 cctgcgctgg ctcaaggagg gcctcggcat cgagaatccg gaaaacggca tcccggaaac
     9781 tgtcccgctg cccgcgccga tggacgggac cgtgatctgg atttccgggg acgcccggcc
     9841 cggcgtggaa ctcgatccca gcacgccgct cctgaccctg ggcgtcatgg acccgatgat
     9901 cgtccgcgcc cacgtgcacg agctggacgt ggtcaagttg aaggtgggcg accgcgcgca
     9961 catgacgctg ctggcccggc cggacgcgaa actggagggc gcgatccggc gcatcgcgtg
    10021 ggcgccgttc ccgcactggc tgcccgagcc gtcctacttc gaggtcgagc tgaccgtacc
    10081 caaccccgac cgtgccctgc gccagggcta caaggtcaac ctgcagttcg gcgacgcggc
    10141 cgcggagtaa cccgtgagcg gacccgccgt ggagattctg cccgtccgca ccgcgcgcga
    10201 gcgcgaggcg tttatccgct tcccgtggca ggtgcaggcc gcggacccgt gctgggtccc
    10261 gccgctggtg tcgcgccagc gcgagtttct cgatccgcgg cgggggccgt tctttgaatt
    10321 cggggaggcg gagtgcttcc tcgcgcggcg gggcgggcga tgggcgggcc ggatctcggc
    10381 gcaggtcagc cgccggcacg acgaattcca cggggccggg acgggtttct tcggcttctt
    10441 cgagtgcgag gacgacccgg aggcggccgg ggcgctgctc cgggccgccg ccgactgggt
    10501 tcgcgcgcgg ggccgccagc gtctggtcgg cccgatgagt ttctccatct acgacgacat
    10561 cgggctgctc gtcgacgggt tcgattccgt gcccgccctc ctgctcacgc acaacccgcc
    10621 gtggtatgcg gaactggtcg cggcgcaggg cttccgcaag gccgtggact ggcacgccct
    10681 tcgggtcacg aacacggaca tcgatcccgc cgcgatgcaa accgaggcgg cggagatcat
    10741 aaagcggagc ggcctcgtgc tgcggatgcc cagcccggcg gaaatggtcc gccgctccga
    10801 cgagatactg gagctgttca acgaggcctg ggacggcaac tgggggcatg tcccgtttac
    10861 ctcgctccag ttcaaggtca tcctcggcga actgcggccg ctgctgcgca ccgacctgat
    10921 ccggctggtc ctggacggcg ggcgcatcgt ggccttcacg atcacgatcc cggacctgaa
    10981 cccgctgatc cgccgcttcg acggtcggct ctcgttgtgg aacatgctgc gcctctaccg
    11041 cgccgcgcag tgggcgccgc tgaagcgggt gaagactgtc ctgctcggcg tgaagcccgt
    11101 cttccggaac cgccgcctgc acgacgcgat gatcctcgac acctacgcgc acctcgtccg
    11161 cacgcagccc ggcatcgagc aatgcgattg ctcgctcatc tgcgagcagt tgcgcgtctt
    11221 catccgctcg ctggagcggt acggggcgag gccgtacaag acgtaccgga tttacgagaa
    11281 ggagatctgg agcaaggcgc ggtagcgagg cggtcgtccc ggtcagggag cgggttcctc
    11341 gcggcgcagc tccagccgga accggcgtcc ctttcgcgtg gtcagcaggc cggagatccg
    11401 gtcctcggcg gtgcgctcgc cctcgaagcg gtggcccgag tggtggacgc cgtgaagtcg
    11461 gttgctttca acgaggccgt tgaagtggta ggtatactgc ccgccgagcg gcccacggac
    11521 ttcggccacg cccatggcct gcgaatccca ttgcgcggcc tgtacggtga tccggaaaaa
    11581 aagcacctgg ccccgccaga tgccggttac cggcgactcg gcgccggatg ctgcggcgca
    11641 cagcaggcaa gtcgcgaaaa tggaggcgcg gaggctcacg gccataaccg gggaagtccg
    11701 acaagcgcga acgagtacga gcccagcagg aagagggcga gcggaacggg ccaggggatg
    11761 cggcggggat cgtccccgga aagcccgagc cccgcgagca ggaagatcgg gagcagggga
    11821 aagagatagc ggccctgggg ctggaagtcg tacatccagc agaacagggc ggcgatcagg
    11881 atgacggcgg cgccggcgag gaccgcgagc agcagcagcg cgagccgggc gggggcccat
    11941 ccgcgccgcc aggcgatcgc cgccgcgagg agcagccccg tgaacagaac ggcctgaatg
    12001 atgtagtagg ccggcggggc caggatgtcc atgtgcccgt atgcgccgcc gaaactgcgg
    12061 gtgatccggc gcgcccaggg ccgctgcgtg agcatatcgg ccagggagac tccctgcgcg
    12121 cgcagggcga gccccgggtc ggcgccgggc gtgccgagcc ggctgggctt gaaggcgtcg
    12181 gccgcgtact cctcccggag cgccgccttg cgttcctcct tctcgaagcg attgatcacc
    12241 gcgtcgcgga cgagcagggg cgccgcgacc atcgcggtaa ggccggctac ggcgagggcc
    12301 cgtttcgggc cggaccgcag cgccaggaac agaacggcga aggcggcgaa gggccagtag
    12361 ttgtgcttgg cgagcagcag caggccgagg agcagggcgg ccggcgcgct ccgccgccaa
    12421 tccgtcggac ccggccaccc gacgaggagc gcgatgagaa aggacaggaa gaaagggaac
    12481 gcgtcgctgt tgaagtagct gaagacatac cagacctgcg gagtccagag caggcacacg
    12541 gccggcagga gcgtgcgcgg ggcgaggcgc caggccgccg ccgccaggac catgaatgcc
    12601 gcgacgctcc acaggcgcag cccgagggcc catcggttgc tgaatccgaa gagccgcgcc
    12661 gatttcgccg cgagcgggta gaggatttgc gggggcgacg ccagcaggta cgacgtgccg
    12721 tacacgggat cgaggtggcc cggcacccgc gggtcgtccg ccgcgggcgg cagccagcgg
    12781 tccaggaaga aacgcgcggt ggtcacgtgc agttcctcgt cgggatgcgc gttcaacggg
    12841 ctgacgagcg ccatgacgag gacctggacg ccggcgagca ggaggaggat gccggccagg
    12901 cggcccggcc gcggcgcgtt caccggaagg ccccgttgag cttctccagg atcgccgggt
    12961 cgcgctcgtc gagccactgc ccgtcgcggt agaagaacca gcgtcccttg cgccgcgtga
    13021 agaaccggtt gtccggcggt tcccgcgcga cgcccaccgg cgccccggcg atcgcgatgc
    13081 cgttctccgg cacggactcc cgcaccaccg cgttggcccc gacgcgggac cgcgcgccga
    13141 ccgtcacgcg cccgacgatc ttcgcgccgg cgccgatgta gcactcgtcg ccgacgaccg
    13201 gcgcgccgag cccggacgaa tcgggcagca cgttcgaccc gatcgtcacc tgctggaaga
    13261 tgacgctgtt tttcccgacg cgcgcatgcc gcgacacgaa gacgccccgg atgccgtgcg
    13321 gcaggcaggg ctttccgccg aactcggcct cgtgcgaaat ccagccgccg ccgagaaact
    13381 ggagcgcgtc gtagatccac accaggacgg cgcgcagcgg cgcccatcgc gtatgacggc
    13441 atacgaggcg aaactcccac aggatgtcgg aatagccgaa catgagggaa gtattaaggc
    13501 acgggggcgg gggagtgaag ctgtttgttg atcacgaacc ggatcttgcc gttggcgtcc
    13561 ggcgcgatgc ggtcgggaaa ctcgaagcgg atcccgagcg ccgcgcccac gcggtcgcgg
    13621 agatgctttt cgagatcggc caggatttcg ggccggaagg cctcgttcct gaccaggcgg
    13681 acgacgatgc cgccgggcgc gtcctggacc acctgcgcga agtcgaaacc cgcgttccac
    13741 ttgaacgcgg cgtccagccg ccccacgcgc cgcccgtcgg gcgtgaccac gacgtcctcg
    13801 atccggccgt cgatggcttc caccaccgtg tgcgcgcggc cgcaggggca ggatttcgga
    13861 ctgatccgcg ccaggtcgcg ggtccggtag cggatcagcg gcatggcgcg gttgaacaag
    13921 ttggtgccgg cgatctcgcc ggtctcgccg ggcggcgcgg accgtccgag cggatcgagg
    13981 atttcgagga tgccgtattc cgcctcgtgg tggtacgagc cccgctcgca ttgcgcgatg
    14041 acgcaggcct gctcccactg gctgtacacg tcgaagacgc ggcagccgaa cgccgcctcg
    14101 atctgctcgc gctgaaacgg cagcaggttt tcagagttgg tcgagatcga acggatgccg
    14161 aggcgccgga gcgcggcggg atcgccgatg aaccggctga attcgtagag cgccgaggga
    14221 tagccgcgca gaaacaccgg cttgaaccgc agcagctcgc cgaggatccg ccgcgccgtt
    14281 tcggccgtca ggttgaagga cgagacgtag agcgcgttca tgctgcgcac ccgccggaac
    14341 agcgcgccgt cgtcgaacac cgggcccgcg atcagcgccc agcgctgccc gatccggtag
    14401 cccgcccggt tccaggcgcg ccagaacgtc gcgaacttgg cgatggccag gtcgcgatcc
    14461 gcgtagaatt ggagaggcgt gcccgtcgag ccgctggtcc ggaccgggac ggggcgcagg
    14521 gtgtcgaatt cgcggggacg caggtcggcg gctcgctcgc gcacgatatc cttggtcagc
    14581 gtcggaatcc gcggcaggtc cgccgctccg cggatgtcgc cgggcttgag cccttcgcgg
    14641 tcgaacagct cgcggtagta gggcacctgc tcgtaggcgt aacggaccag ctcgcgcacg
    14701 cgcgcgtcct gccagctctt gagtcggtcc gccggccagc gctcggcgcg cgcgaggaag
    14761 cggcggtgcc ggacgatgcg ccaggggtgg aagaaggtgc ggagattgaa atcggtgatc
    14821 attcgatgac gatccggagg ccccgcagcg cgcggtcgag ggccgcgagc gcatccccgg
    14881 gccgggcccc cttcgcgatg atatgcccga tccggtcggg accgacccgg aagcgccgaa
    14941 cgcggtcgcc gggccggtaa tcgaacgaga cgtccacgat ctccgggtcc ggcccgtttt
    15001 cattgcgctg ttcgcggatc acgccgtccc ggtccgcgcg caggaggacg gccgcgcacg
    15061 gcgtgcccgt ccccgccggg aattccggcg tgccgcccag cgcggcgcgc aggatctgct
    15121 cgtagtagtt gaagccgccg taaatcccgg ccagttcggg caggcatgtg gcgccggccc
    15181 gggcgccgaa ctcgatgacg tggacgccgg cctcgttgac gatgaagtcg aaattcatgg
    15241 cgcagtgatc caggcgggac gcgcgcacgc agcgctcgac ctggtcccgg acttccgcca
    15301 gccgggccag cccttccgaa tggggcaggt ggtgaccgat aggcacgtgc gcttcccctt
    15361 cgaagaccgt gcagccggtg ggcaggaggt agaccacgcg accgttcagg acaaacgcct
    15421 cggcgccgca gtccgcgccc tcgatgaact gctcgacgag gaactcctcg cgccgagtaa
    15481 cggcgcggac cttggcgacg gcggaatcga tctcgccggt cccgcggacc ttcacgacgc
    15541 cgcggctgcc ggagctgtcc acggccttga ccatgaccgg ggtgcccaat tcccgcgcgg
    15601 cggtcagcgc ctccgcggcc gtccgcgccg cgcggaactc cggcatccgg accccggcct
    15661 gccgcccggc ccgccgcatg agcagcttat tcgtcgcgat ggccgtcgcc gccgcggaag
    15721 ggccgggaac gcccagggcg tccacgacgc cacccagcgc cgcgagggcg acgtcggtgc
    15781 cggaggtgca gatgccgtcg atcttttcgg cccgggccac gccgagcacc gccgccgcgt
    15841 cggtggtgtc cacgtggaac gcgcggtcgg cgagcgcgaa gccggggtaa gggccgggcc
    15901 ggctggcgac gagcacgcgc aatcccatga tgcgcgcctg ccggatcagc ggcgcctggt
    15961 agatgcccgc gccgaggatg aagatcgttt tatccgttgc cggttcgggt ttcatgtgtc
    16021 cgtggacgca tccatccctg ggcggccatc agtataacgc cgatcgacgt gttgaacagc
    16081 aggcctgcga ggttgaagaa actgaccagg agcgcggtct ccggcggcac gccgaacagg
    16141 ccgtagagca ggatgtacgc gccctcgcga atcccgaggc tgccgaagga aatcggcagc
    16201 aggaacacga acatgagcac cggcaacacg aaaagattcg cgaggtaggg aatcggccag
    16261 ccgacggcgc ggaagaatat ctggttgccc agcgcggcga tcagcaggtt ggccagcgac
    16321 aggcccagcg tgccccgcag caggcgccac gaggtcacgg gctcgatcag cgacggaggt
    16381 tccgtcccgg gcgcgcgccg caacagccgg gcggccagcg cgctgacgaa gcggtcgagc
    16441 cggcgccggg ccaggagcag gaacaggaac aacaggccgg ccgccccgag cagcgcgcag
    16501 gccgtcgtca gcaccgcccg ggcggccggc ccggcctcga tgggcagcag cgggtagagc
    16561 ccgacgacca ggaccgcgca cgtgacgagc gccaggagtt tctccgtgac gatcgccgcc
    16621 gcgccgggcg cgtaccgtcc gaaatgccgt ccggcggcaa cgagccggta cgcgtcccac
    16681 ccgatggaag ccgggccgaa caggccgagc gccagcccga tccagtagtg cttcagcgcg
    16741 aacccgtatc cgacccgttc ccggtgaaac tgctcgagga gcagccgcca gcgcagcgcg
    16801 ccgaccagga tgaccagcgg gtagtacagc aggccgagca gcaccaggcc gggagccgcg
    16861 cgcgtaaaat tctcgcggaa catgggccag tcgatccggc ggaagataac ggccaggatc
    16921 aggaccggca ggagccagaa gagagcctgg cgaaggaccg gaagcaggcg aaatccggcg
    16981 ccctgcgcgc gcaggccccc gcgcagggcg gcccaggtca gccggagccc gtgacgacgg
    17041 accatgtcca ggtaggcctc caggttgggc ccgagaaagg aaggccggag caaagtctgc
    17101 gcgcgcgcgg ccgcgagcgc cggcgggcgg gcgatgcgg
//
LOCUS       DAOOTK010000060        17131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2292_length_17131_cov_6.822558, whole genome shotgun sequence.
ACCESSION   DAOOTK010000060 DAOOTK010000000
VERSION     DAOOTK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17131
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2292_length_17131_cov_6.822558"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..658)
                     /locus_tag="PK634_08125"
     CDS             complement(131..658)
                     /locus_tag="PK634_08125"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOW97738.1"
                     /translation="MKHANLILVGFMGAGKSATGRRVAARLGRRFVDMDAVIEARAGR
                     AIPEIFACEGEAHFRKLERALVLELAGQRDLVIAAGGGVVLNPDNLRDFERTGLVVCL
                     SAALETILKRVGHHRNRPLLEGGDKEQRVRELLENRRALYEAVARQVETTDLTPDEVA
                     ARVLALYGEAPEAPV"
     gene            complement(744..965)
                     /locus_tag="PK634_08130"
     CDS             complement(744..965)
                     /locus_tag="PK634_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOW97739.1"
                     /translation="MATGTVKWFNEKKGYGFIIPDGMNQDVFVHYSAIMSEGFKTLNE
                     GDKVQFEIFQDAKGARARDVTLVNAVAAS"
     gene            complement(1160..1984)
                     /locus_tag="PK634_08135"
     CDS             complement(1160..1984)
                     /locus_tag="PK634_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOW97740.1"
                     /translation="MSDVPPLLWGYLTALVFAFGACVGSFLNVGIYRIPREESVIAPR
                     SHCPHCGKLIAWHDNIPLVSWLVLRARCRQCGEKISIRYFLVESLTAVLFLLVWREYG
                     VDPRTPVYLLIMAGLVLGTFVDLEHLIIPDRVSLGGIAAGLILSPLVPSLHGVTGALA
                     GFKMALIGLAAGAGVLWLVGVLGKLAFKKDAMGLGDVKLLGGLGALLGWQAVVFIIMA
                     SSLVGSLVGVGMIVSGHKEWQSRVPYGPYLAVAAGVWILWGFRWWAAYVTWLRPPM"
     gene            complement(1981..2841)
                     /gene="aroE"
                     /locus_tag="PK634_08140"
     CDS             complement(1981..2841)
                     /gene="aroE"
                     /locus_tag="PK634_08140"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOW97741.1"
                     /translation="MPITGHTKIYAVLGHPVAHTLSPAMHNAAFAALGLDAVYVALDV
                     TPEKLPGALAALADLGFGGLNVTVPLKEVACRALPNLDESARLMGAVNTVQFTPAGPV
                     GHNTDGAGFLRAAEEAFGAPVAGRSMFILGCGGAGRAVALASAGAGVTKFALADLDAG
                     RVRKLAMELETQFMVSEARVIEASSELAGAARAADLVVQATPAGLKPGDASPLGAGAF
                     RAGQWAFDLVYGAPETPFMREAKKSGATAANGLGMLLHQGAKAFEIWTGRAAPADVMR
                     RALEQKVYGA"
     gene            complement(2863..3042)
                     /locus_tag="PK634_08145"
     CDS             complement(2863..3042)
                     /locus_tag="PK634_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97742.1"
                     /translation="MARYSDVFQTLEKVLSIFSKHWKNSGEIFQTLEKPAEKFPMFGK
                     NEGKISNVWKTPPEP"
     gene            complement(3054..3620)
                     /gene="def"
                     /locus_tag="PK634_08150"
     CDS             complement(3054..3620)
                     /gene="def"
                     /locus_tag="PK634_08150"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOW97743.1"
                     /translation="MKKTIHTYGDAVLRKKARPVEKVDDAIRALVRDMLDTMREERGV
                     GLAAEQVGEDVAVCVIEVPPEMDRDDKGLFFNPGATMPLALINPKIVAASRDTETAEE
                     GCLSFPGIYAPITRPVEVTVEYLDTQGKARAQTFRKFVARAVQHEMDHLNGVLFVDHM
                     SALKKIALAGQLKRLKRETKEAMKETNL"
     gene            3714..4475
                     /locus_tag="PK634_08155"
     CDS             3714..4475
                     /locus_tag="PK634_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOW97744.1"
                     /translation="MNEALVVIPTYNEKENIRGVVAAVEKACPSADLLFVDDNSPDGT
                     GRIADEIAAGDPRVHVLHRERKQGLGRAYLAGFRWGLERHYQFLFEMDADFSHNPNDL
                     PRLLEAAGGADLVLGSRFVGGIRVINWPLSRLMLSKGAALYVRIITGMPFMDPTGGFK
                     CYRRAVLEAIPLDEIKSNGYSFQIEMTHQAWRRGFQIVEAPIIFEERRSGQSKMSGGI
                     VGEALWLVWKIFMRNGLRRRPGPAHPRSIAAKAAS"
     gene            4472..5374
                     /locus_tag="PK634_08160"
     CDS             4472..5374
                     /locus_tag="PK634_08160"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOW97745.1"
                     /translation="MSANPLWSAVRAMRPKQWTKNAVVLAAFIFALGDRQQGVAISAI
                     WTAIGAALLFCLASSAVYLFNDVRDLPQDRLHPKKKLRPIAAGELPVPVALGMSAILL
                     IAALQGAWLVSRDLALVIGGYVALQIVYTLALKKVALVDIFMIAGGFVLRALAGAVAI
                     RATISPWLLLCTLLLALFLALCKRRHEKVVLSDVAGATRESMGQYDERLLDQLIAIVS
                     AATLVCYALYTLWPDTVQKFGTAKLGLTIPFVIFGLFRYLDIVYRHEKGDRPEQILLT
                     DVPLLADLFLYGLTVLGVLLYGRP"
     gene            5686..6645
                     /locus_tag="PK634_08165"
     CDS             5686..6645
                     /locus_tag="PK634_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW97746.1"
                     /translation="MPESKPVTLVTGGAGFLGSHLCDLLLGKGHRVIAMDNLITGNLA
                     NIAHLEGRNDFQFIHHNVTEYIDIPGPVDIVFHFASPASPIDYLELPIQTLKVGALGT
                     HKTLGLARAKGSRFLLASTSEVYGDPLVHPQPETYWGNVNPIGPRGVYDEAKRFAEAM
                     TMAYHRFHGVDTRIVRIFNTYGPRMRPRDGRVVPAFITEALKGEPITVFGSGRQTRSF
                     CYVADLIAGIYKLAMSDCHEPVNIGNPSEMTVLDFAEKIRGLCRATSPIVFKPLPEDD
                     PKIRQPDIRKARAVLGWEPATNLDQGLALTIEYFRGLLAKGLI"
     gene            6707..7261
                     /locus_tag="PK634_08170"
     CDS             6707..7261
                     /locus_tag="PK634_08170"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOW97747.1"
                     /translation="MRLRYAKKDGTQMEFELGDRPITIGRSPEADVVVLDEKVSRVHC
                     GIRLWDGEFFIKDLKSRNGTTVNGQRVEMVKLNPGDRIRVGSTTFLFEQEAGPQTDDA
                     LKEMQQAMADGKGYSTILQEIVEDIAAPAAPEGAPPGPAPADQSASPMPPPEKPAPPK
                     TVVKKPAAPKAGAPFKVVIRKPAP"
     gene            7328..10654
                     /gene="ygfK"
                     /locus_tag="PK634_08175"
     CDS             7328..10654
                     /gene="ygfK"
                     /locus_tag="PK634_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOW97748.1"
                     /translation="MSDKFQPLTTEQLARWILDELEGSNSIFGIPRDLFFRPRPDDSF
                     RASVYGKTLETPFGVAAGPHSQMAQNIIIAWLCGARFIELKTVQTLDELEVSKPCIDM
                     QDEGYNCEWSQELKVHESFEEYLRAWILIHLLHHKLGLPGKEPGIVFNMSVGYNLEGL
                     QKPNMQWYLEKMHDAGEDLARDLDIIARRDPAVRSLNIPARMSDNVTLSTMHGCPPGE
                     IEQISKYLLETWGLHTSVKCNPTLIGPADLRRILNQDLGFRDVTVPDIAFEHDLKYAD
                     AVPMLRRLGDAARARNLQFGVKLSNTLEVENWRPIFPAKEKMMYMSGRALHAVTTNLA
                     AKLGREFGGKLMMSFSAGADAFNVADLLAAGMRTITVCSDILKSGGYLRIVQYLDHVR
                     DAIKAAGAKDIPGLIAKKSGGIAGEAGALANLDRYAAAVLKDPRYRKDRFDTSRTKTT
                     RALDLFDCIEAPCTDECPINQKVPQYMTLVREGRVDEAVEITRLDNPLPSVLGRACNH
                     LCENTCIRTHYDDPLAIREMKRFIMDHEVKPHYRPQAPARDVKVAVVGGGPMGLSTAY
                     FLGQAGYQVTVFEARPYVGGMVSGTIPAYRATDKVIEQDLNVIRQLGVEFRFGQEAGR
                     DFTLADLRKQGYRYIALGVGAQKGKTLGVEGETNAGVLDALQFLRDSREGHPPPLGKR
                     IGVIGGGDVAMDCARSAWRLTGAKVTIVYRRTIAEMPAAPEEIAGVLEEGIEIMELVK
                     PLRVIAKDGKMTALEVRKMKLGEKDESGRRRPVDIPGSDFQLPLDNLIVAISQEPSLK
                     FLEGEPVERNKSGYLKVDRVTMETSAPGLYAGGDVVGDGPESIVMAMGDGRKIADAIR
                     RKEETIEERRAAWPAVNETELIRRKARREYRVHLPHRDPADRFNFEEVQLTLSEEEAK
                     REASRCLDCHTYCSLCVSVCPNRAILTYKVKAFERRLADLAARGGKGVASPGTTVRVD
                     QPFQVAVLTDFCNECGNCETFCPTAGAPYKMKPRLYLRRKEFDAEQDNAFMVFRANGH
                     MGIRGRKAGQTHEFVVAGDELRYSAPGWRVKMGTDFSVREAAATGAADGDVLGLDFCA
                     KMAVLLDGLTTSQPALPVAT"
     gene            complement(10667..13183)
                     /gene="mutS"
                     /locus_tag="PK634_08180"
     CDS             complement(10667..13183)
                     /gene="mutS"
                     /locus_tag="PK634_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOW97749.1"
                     /translation="MTDAATPMMSQYRRIRRDLPPDAILFFRLGDFYEMFFEDAKDAS
                     AILDIALTKRQGVPMCGVPYHAADLYLAKLIRAGRKVAICDQVEDPAVARGIVQREVT
                     RIVTPGTVLEENVLESARHNYLAGVGLAGDRIGVALLDLSTGSFTIEEHADPVALRDP
                     LAQAAPSECVVPETAKDDPRVRAVLEACGQTLVTPYEDWTFEPVSATELLARHFEVHS
                     LAGFGCDDRPAAVGAAGAVLHYVVHELRRQVGHIRQLRLRNPAEFMVLDESTAANLDL
                     VASRGPARAGVATTLLRVLDSTRTPMGARLLREWVLRPLTDLGQIVQRHDAVEALFRD
                     RPRLADLRERLGRVRDLERLIARLGAGAGNARDMVGLGGSLVELPGLRDVLAGAPAGP
                     LPDLAAHIRPLPELVDLIARAIVDEPPAVIKDGGLFRKGYHPELDELRAAATEGRQWL
                     ADFQAAEQQRTGIKSLKVRHNKVFGYYIEITKSNLDAVPADYIRKQTIVNAERFITPQ
                     LKEYENKILGAQERALALELELFAELRAAVVKETAAIQQSAEALAAADCLSSLAERAL
                     ALGYVRPAMNGGDALKIREGRHPVIEQMPESERFVPNDTVMDGTANQLIIITGPNMAG
                     KSTYIRQVALIVIMAQVGSFVPAASAELGVVDRVFTRVGASDDLARGRSTFMVEMQET
                     ANILNNATPRSLIVLDEIGRGTSTFDGISIAWAVAEHLHNHAPVKAKTLFATHYHELT
                     DLALTMPGVKNYNVLVRERGDKIAFLRRIVPGAADKSYGIAVGRLAGLPAAVVDRARE
                     ILANLEEGEFGEGGQPKLARHGGGRKRLRDPNQMNLFGQE"
     gene            complement(13229..13525)
                     /locus_tag="PK634_08185"
     CDS             complement(13229..13525)
                     /locus_tag="PK634_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97750.1"
                     /translation="MASEEETNLYGVEPGCPTHGDEKMRECSMCGAEYCRACHPRSTV
                     CPDCAEPSDDDEEEADPDFEDVNDLDEVLEDDEEEEKFDNGEDDLRGGGKDREY"
     gene            complement(13548..14162)
                     /locus_tag="PK634_08190"
     CDS             complement(13548..14162)
                     /locus_tag="PK634_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOW97751.1"
                     /translation="MSIEAMQHPVLVLNRLWQAINVCTADRALALLYTGHAQAVCETP
                     TGFGTFSFREWCDFSPTAGEAGAVRTISLRIRIPRIILLLFFDRLPNKEVKFTRHNVF
                     ERDRNTCQYCGKRFDRRDLNIDHVVPRQAGGLTQWTNVVCSCVDCNRLKANRTPEQAG
                     MRLIRKPKKPRWRPFVEIQFVRTADHSWRHFLDLAYWNVELGED"
     gene            14281..15480
                     /locus_tag="PK634_08195"
     CDS             14281..15480
                     /locus_tag="PK634_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW97752.1"
                     /translation="MKSAFLDKLMERLDRVDPGSLQTHFLRLAREKGLLETLFRAVRE
                     GIIVLDGRGRITYANRAAEQMLGFQADAAAGQSIRRFLREMDWDRLLELDPEEWSRLV
                     SREIEITYPERRLLDFYVVPLTAVAESERGAVVLLRDVTSDRDHEARAVESERLNALT
                     LLAAGVAHEIGNPLNSLHIHLQLIERELGGLPPEKRRELVDLVEVSRREVERLDGILT
                     QFLRALRPAPPNLAPGSVADVLRETLNFLQHEIRDRDVLVEVEAPEALPAIPIDADQI
                     KQAFFNIIRNAIQAMPGGGLLKITLRETDRFVEIAFRDRGPGIPPEDIHRIFEPYQTT
                     RPDGSGLGLAIVQRIVRDHGGQIEVHSRPGEGTTFTLLLPRTGQRLRLLQASPDLEPA
                     APDGGPP"
     gene            15477..16847
                     /locus_tag="PK634_08200"
     CDS             15477..16847
                     /locus_tag="PK634_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOW97753.1"
                     /translation="MKKQPVILIVDDEKNTRDGLARALGRNYAVRLADSAARALQILD
                     EQPADVLLSDIRMPGEDGLALMQRALARTPAPVCILLTAYGSIETAVDAMKRGAYDFL
                     TKPVSLDRLELLLQRALRERDMESENRRLREQLDARFGLEQLIGRSAPMQEVFDSIRQ
                     VGPSRATVLVTGESGTGKELVARAIHQLSPRVHGPFIPLHCAALAPTLLESELFGHEK
                     GAFTGAVERRRGRFELADGGSLFLDEVGEIPAAIQVKILRVLEERKFERVGGQETVEV
                     DVRLIAATNRDLKKMVDEGTFREDLYYRLNVVTIHLPPLRERPDDIPLLVQHFLKELS
                     AENGKTIAGLTPEAMAALAAHTWPGNVRELRNVIERMVVMARGDTLTVRDIPAALRDE
                     SRPRGGLPAGPVSLDEAEKQLILRALKACDDNRTAAARQLGISRRTLHRKLKEYGLAE
                     DPPSSP"
BASE COUNT         2780 a         5897 c         5757 g         2697 t
ORIGIN      
        1 cgtgtccgtc tgcggcgccg tcgcctcgtg tccggcttcg atgggtgctt tttctgccgc
       61 catggccgta tcctctcggt tggaaaactc cgcgtgttta ccccttgccc cgccaaaagg
      121 caactgcgga ttacaccggc gcctccggcg cttcgccgta cagcgccagg acccgcgcgg
      181 cgacctcgtc cggcgtgaga tcggtcgtct ccacctgccg ggcgacggcc tcgtacaggg
      241 cgcgccgatt ttccagcagt tcccgcacgc gctgttcctt gtctccgccc tccaggagcg
      301 ggcggttccg atgatgcccc acgcgtttca ggatggtttc cagcgcggcg gacaggcaga
      361 ccaccagtcc cgtccgctcg aaatcgcgga ggttgtccgg gttgagcact acgccgcccc
      421 cggcggcgat caccaggtcg cgctgcccgg ccagttcaag caccagcgcg cgctccagct
      481 tccggaagtg ggcctctcct tcgcaggcga agatctcggg gatggcgcgg ccggcgcgcg
      541 cctcgatcac cgcgtccatg tccacgaacc ggcgcccgag ccgcgcggcc acccgccggc
      601 ccgtggcgga tttgccggcg cccatgaaac cgacgaggat gaggttggcg tgtttcatcg
      661 cgagcccggg caaagcgccg cagatccccg gaacactccg gggatccgtg gcggcatgag
      721 acacaagcgc ggcaggtccg cccttacgag gcggcgacgg cgttgaccag cgtgacgtcg
      781 cgggcccggg cgcccttggc gtcctggaag atctcgaact ggaccttgtc cccttcgttc
      841 aaggtcttga acccctccga cataatggcg gagtagtgga caaagacatc ctggttcatt
      901 ccgtccggaa tgatgaaccc gtaacctttt ttctcgttga accacttcac agtaccggta
      961 gccataacaa ttacttcctc cacattgcta gagccgcgca ttgtgccgtc cctttccagg
     1021 aacacaagag aaatgtttgg ctcgatgaaa tggggcgtcg ccgcatgaag gcggggcgcc
     1081 ctgaccccgg gaaaccgtca agcgaagcgg cgcgggagag gcgtcgcgcc tccggatccc
     1141 ggcgcggact ccggcgccgt cacatcggcg ggcgcagcca tgtcacgtac gccgcccacc
     1201 agcggaagcc ccacaggatc cacaccccgg cggccaccgc gaggtacggc ccgtacggca
     1261 cgcggctctg ccactccttg tgcccgctga cgatcatacc cacgcccacg agcgagccga
     1321 ccagcgacga ggccatgatg atgaacacca ccgcctgcca ccccagcagc gcgcccagcc
     1381 cgccgagcag cttgacgtcg cccagcccca tggcgtcctt cttgaacgcc agcttgccca
     1441 gcacgcccac cagccacagc accccggcgc ccgccgccag cccgattaac gccatcttga
     1501 acccggccag cgcgccggtc acgccgtgca gcgagggcac aagcggactc aatatcagtc
     1561 ccgccgcgat cccgcccagg gatacgcggt ccgggatgat caggtgctcc aggtccacga
     1621 acgtgccgag gaccaggccc gccatgatca acaggtatac cggcgtgcgc ggatccacgc
     1681 cgtattcccg ccacaccaat agaaagagaa ccgcggtgag cgattccacg agaaaatacc
     1741 ggatcgatat tttttccccg cactgccggc agcgcgcccg caggaccagc cagctgacga
     1801 gcgggatgtt gtcgtgccac gcgatcagct tcccgcagtg cgggcagtgc gagcgcgggg
     1861 cgatcacgga ctcctcgcgc gggatgcggt agatgccgac gttcaggaag ctgccgacgc
     1921 acgcgccgaa ggcgaagaca agggccgtca ggtaccccca gaggagcggc ggcacgtcgc
     1981 tcatgcgccg tacaccttct gctcgagcgc gcgccgcatg acgtccgccg gcgcggcgcg
     2041 gccggtccag atctcgaacg ccttcgcgcc ctggtgcagc agcatgccca gcccgttcgc
     2101 cgccgtcgcg ccgctcttct tggcctcgcg catgaacggc gtttccggcg cgccgtagac
     2161 gaggtcgaac gcccactgcc cggcgcggaa cgcccccgcg cccagcggcg aggcgtcgcc
     2221 cggcttcagc cccgcgggcg tcgcctgcac gacgagatcc gccgcgcgcg cggcaccggc
     2281 gagctcgctc gacgcctcga tgacgcgcgc ctcggagacc atgaactggg tctcgagttc
     2341 catcgccagt ttccggacgc gccccgcgtc gaggtcggcc agcgcgaact tcgtgacccc
     2401 ggcgcccgcc gaggcgagcg cgaccgcccg gcccgcgccg ccgcagccga ggatgaacat
     2461 cgagcggccc gcgaccggcg cgccgaacgc ctcctcggcc gcgcgcagga aacccgcgcc
     2521 gtcggtattg tgcccgaccg gcccggcggg cgtgaactgg acggtgttca cggcgcccat
     2581 caggcgggcg gactcgtcga ggttcggcag cgcccggcag gcgacctctt tcagcggcac
     2641 ggtgacgttc agcccgccga agccgaggtc cgccagcgcg gccagcgcgc ccggcagctt
     2701 ctccggcgtc acgtcgagcg cgacgtagac cgcgtcgagc cccagcgcgg cgaacgcggc
     2761 gttgtgcatc gccggcgaca gcgtatgcgc gaccgggtgg ccgaggacgg cgtaaatctt
     2821 cgtgtggccg gtgatgggca tggggaggga agggttcaga gttcagggtt cagggggcgt
     2881 tttccaaaca ttggaaattt ttccttcatt ttttccaaac attggaaact tttccgccgg
     2941 tttttccaat gtttggaaaa tttcgccgct atttttccaa tgtttggaaa aaatggacag
     3001 cactttttcc aatgtttgga aaacatcgct gtagcgcgcc acgccgcgcg gcgctacaga
     3061 ttcgtttcct tcatcgcctc cttggtctct cgcttgaggc gcttgagctg gccggcgagg
     3121 gcgatcttct tcagggcgga catgtggtcg acgaagagca cgccgttgag gtggtccatc
     3181 tcgtgctgga cggcgcgggc gacgaacttg cggaaggtct gcgcccgggc tttgccctgg
     3241 gtgtcgaggt actcgacggt gacctcgacg ggccgcgtga tgggcgcgta gatgccgggg
     3301 aagctcaagc agccctcctc ggcggtctcg gtgtcgcgcg aggcggcgac gatcttcggg
     3361 ttgatcaggg cgaggggcat ggtcgcgccg gggttgaaga acagcccctt gtcgtcgcgg
     3421 tccatttccg gcgggacctc gatgacgcag acggcgacgt cctcgccgac ctgctcggcg
     3481 gccaggccga cgccgcgctc ctcgcgcatg gtgtcgagca tgtcgcgcac gagggcgcgt
     3541 atggcatcgt ccaccttctc caccggccgc gccttcttgc gcagcacggc gtcgccgtag
     3601 gtatggatcg ttttcttcat gcgcgttccg aactcaccat acgcattccc gcgcggattt
     3661 caacggcgta aacgcttgcc gacccggggg gagtgtccta cattgggggg gcgatgaacg
     3721 aggcgctcgt cgtcattccg acgtacaacg agaaggagaa catccgcggc gtcgtggcgg
     3781 ccgtggaaaa ggcgtgcccg tcggcggacc tcctgttcgt ggacgacaac tcgccggacg
     3841 gcacggggcg gatcgcggac gagatcgcgg ccggcgaccc gcgggtgcac gtgctgcacc
     3901 gcgagcgcaa gcaggggctg ggccgggcgt acctcgcggg attccggtgg ggcctggagc
     3961 ggcactacca gttcctgttc gagatggacg ccgatttttc acacaacccg aacgacctgc
     4021 cgcgcctgct ggaggccgcc gggggggcgg acctcgtgct gggttcgcgg ttcgtcgggg
     4081 gcatccgggt gatcaactgg ccgttgagcc ggctgatgtt gagcaagggc gcggcattgt
     4141 atgtccggat catcacgggg atgccgttca tggacccgac gggcgggttc aagtgctatc
     4201 ggcgggccgt gctggaggcc atcccgctgg acgagatcaa gtcgaacggc tactcgttcc
     4261 agatcgagat gacgcaccag gcgtggcggc ggggattcca gatcgtggag gcgcccatca
     4321 tcttcgagga gcgccggtct ggccagtcga agatgagcgg cggcatcgtc ggggaggcgt
     4381 tgtggctggt gtggaaaata ttcatgcgca acggattgcg gcggagacct ggtccggccc
     4441 atccccgcag catcgcggcg aaggcggcgt catgagcgcg aatcccctat ggagcgcggt
     4501 tcgcgcaatg cggccgaagc aatggaccaa gaacgcggtg gtcctggcgg cgttcatttt
     4561 cgcgctaggc gaccggcagc agggcgtggc gatatcggcc atttggacgg cgatcggggc
     4621 ggcgctgctg ttctgtctgg cttcgagcgc ggtatacctg ttcaacgacg tccgcgatct
     4681 tccgcaggac cggcttcatc ccaagaaaaa actccgtccg atcgcggcgg gcgagttgcc
     4741 cgtgccggtg gcgctgggca tgtcggccat cctgttgatc gcggccctgc agggggcgtg
     4801 gctcgtgtcg cgggacctgg cgctggtcat cggcggctac gtggcgctgc aaatcgtcta
     4861 tacgctggcg ctgaagaagg tcgcgctggt ggacatcttc atgattgcgg gcgggttcgt
     4921 gctgcgggcg ctggcgggcg cggtggcgat ccgggcgacg atttcgccgt ggctgctgtt
     4981 gtgcacgctg ctgctggcgc tgttcctcgc gctgtgcaag cggcgccacg agaaggtcgt
     5041 gctcagcgac gtggccggcg cgacgcgcga gagcatgggg cagtacgacg agcggctgct
     5101 ggaccagctc atcgccatcg tcagcgccgc gacgctggtc tgctatgccc tgtacacgct
     5161 gtggccggat acggtgcaga agttcggcac ggcaaagctc ggcctgacga tcccgttcgt
     5221 gatattcggc ctgttccgct acctggacat cgtgtaccgc cacgagaagg gtgaccggcc
     5281 cgagcagatc ctgttgacgg acgtgccgct gctggccgac ctgttcttgt acggcctgac
     5341 ggtcctcggg gtgctgctgt acgggcggcc gtaggttctg cccctgctca aacgggccgg
     5401 ttccagccag cgctccgcat atcacacttt gcaaagtgtg gaattccatg cccgccagta
     5461 taagcatttt tcattaatta cttgccgatt tttaagattc tgtttttttc acactttgca
     5521 aagtgtgaaa atacctaggg agggcggcgc ggctccgaca ggatagccga aggcgcgcag
     5581 aggaccggcc tgcggatatc gcaacggtcc cgaacgaggg ctgggaaccc cgccctcgtt
     5641 tgcatttgac gggcgcgccg acagtcgtta gcgtgtgctg ccgtcatgcc ggaaagcaaa
     5701 ccagtcacgc tcgtcaccgg aggcgccgga ttcctcggct cccatctctg cgacctgctg
     5761 ctcggcaagg ggcaccgcgt gatcgccatg gacaacctga tcaccggcaa cctcgccaac
     5821 atcgcgcacc tcgagggaag gaacgacttc cagttcatcc accacaacgt caccgagtac
     5881 atcgatatcc ccggccccgt ggacatcgtc tttcacttcg cgtcgccggc cagcccgatc
     5941 gactacctgg agttgccgat ccagaccctg aaggtcggcg cgctgggcac ccacaagacc
     6001 ctcggcctgg cgcgggccaa gggctcgcgg ttcctcctgg cctccacctc ggaggtctac
     6061 ggcgacccgc tggtccaccc gcaacccgag acctactggg gcaacgtcaa tcccatcggc
     6121 ccgcgcggcg tgtacgacga ggccaaacgc ttcgccgaag ccatgaccat ggcctaccac
     6181 cgcttccacg gcgtggacac gcggatcgtc cgcatcttca acacctacgg cccccgcatg
     6241 cggccgcgcg acgggcgcgt cgtgccggcc ttcatcaccg aggccctcaa gggcgagccg
     6301 atcaccgtct tcggcagcgg ccgccagacc cgcagcttct gctacgtcgc ggacctgatc
     6361 gccgggatct acaagctggc catgagcgac tgccacgaac cggtcaacat cggcaatcca
     6421 agcgagatga ccgtgctgga cttcgccgag aagatccgcg gcctgtgccg cgcgacctcg
     6481 ccgatcgtct tcaagccgct gccggaggac gatcccaaga tccgccaacc ggacatccgc
     6541 aaggcccgcg ccgtgctggg ctgggagccg gccacgaacc tggaccaggg cctcgccctg
     6601 accatcgagt atttccgcgg cctgctcgcc aagggcctga tttagcggct tgcgtcccgc
     6661 ccggcggata ccctaggata caccccagtc aacgggacga agcaccatgc gattgcgata
     6721 cgccaagaag gacggcaccc agatggagtt cgaactgggc gaccgcccca tcaccatcgg
     6781 gcggagcccc gaggccgacg tggtcgtgct ggacgaaaag gtatcccggg tccactgcgg
     6841 catccgcctc tgggacggcg aattcttcat caaggacctc aagtcccgca acggcacgac
     6901 ggtgaacggc cagcgcgtgg aaatggtgaa gctcaacccc ggcgaccgga ttcgtgtggg
     6961 ctccacgacg ttcctgttcg agcaggaagc cggcccgcag acggacgacg ccttgaagga
     7021 aatgcagcag gccatggcgg acggcaaggg ctacagcacc atcctgcagg agatcgtcga
     7081 ggacatcgcg gcccccgccg ccccggaagg cgccccgccg gggccagccc cggccgacca
     7141 gtcggcaagc cccatgccgc cgcccgaaaa acccgcgccg cccaagaccg tggtcaagaa
     7201 gccggcggca cccaaggccg gcgcgccctt caaggtcgtg atccgcaagc ccgcgccgta
     7261 acgtccggcg ctccgccgtc gaccggcgtt cgggagtttt gtgcgaagat agaccggagg
     7321 aaagaccatg tccgacaagt tccaacccct cacaacagag caactggccc gctggatcct
     7381 ggatgagttg gagggctcga actccatctt cggcatcccg cgcgacctgt tcttccggcc
     7441 gcggcccgat gattccttcc gcgcgtcggt ctacggcaag accctcgaga cgccctttgg
     7501 cgtcgccgcc ggcccgcact cgcagatggc ccagaacatc atcatcgcct ggctctgcgg
     7561 cgcgcggttc atcgagctta agaccgtcca gacgctcgac gaactcgagg tctccaagcc
     7621 gtgcatcgac atgcaggacg agggctacaa ctgcgagtgg tcgcaggaac tgaaggtcca
     7681 cgagtcgttc gaggaatacc ttcgggcgtg gatcctgatc cacctgctcc accacaagct
     7741 gggcctgccc gggaaggagc ccgggatcgt cttcaacatg agcgtcggct acaacctcga
     7801 ggggctgcag aagcccaaca tgcagtggta cctcgagaaa atgcacgacg cgggcgagga
     7861 cctggcccgc gatctcgata tcatcgcgcg ccgcgacccg gccgtccgct cgctcaacat
     7921 cccggcgcgg atgtcggaca acgtgaccct ctccaccatg cacggctgcc cgccgggcga
     7981 gatcgagcag atctcgaaat acctgctgga gacatggggc ctgcacacga gcgtcaagtg
     8041 caaccccacc ctgatcggcc cggccgacct gcgccgcatc ctgaaccagg acctcggctt
     8101 ccgcgacgtc accgtgccgg atatcgcctt cgagcacgac ctgaagtacg ccgacgccgt
     8161 gcccatgctg cgccggctcg gcgacgccgc ccgcgcgcgg aacctccagt tcggcgtgaa
     8221 actgtccaac acgctcgagg tcgagaactg gcgcccgatc ttcccggcga aggaaaagat
     8281 gatgtacatg tccggccgcg cgctgcacgc ggtcacgacc aacctcgcgg ccaagctggg
     8341 ccgggagttc ggcggaaagc tgatgatgtc cttctccgcc ggcgcggacg cgttcaacgt
     8401 cgccgacctt ctagcggcag gcatgcggac gatcaccgtc tgctccgaca tcctcaagtc
     8461 cggcggctac ctgcgcatcg ttcagtacct ggaccacgtc cgcgacgcga tcaaggccgc
     8521 gggcgcgaag gatatccccg gcctcatcgc gaagaagtcc ggcgggatcg ccggcgaggc
     8581 gggcgcgctg gccaacctcg atcgctacgc agcggccgtc ctcaaggatc cgcgctaccg
     8641 caaggaccgc ttcgacacct cgcgaaccaa gaccacgcgc gcgttggacc tcttcgactg
     8701 tatcgaggcg ccgtgcaccg acgagtgccc gatcaaccag aaggtcccgc agtacatgac
     8761 cctggtccgc gaggggcggg tggacgaggc cgtcgagatc acgcggctgg acaaccccct
     8821 gccgtcggtc ctcggccgcg cgtgcaacca tctctgcgag aacacctgca tccgcacgca
     8881 ctacgacgac ccgctggcca tccgcgagat gaagcggttc atcatggacc acgaggtcaa
     8941 gccgcactac cggccccagg cgcccgcgcg cgacgtcaag gtcgccgtgg tcggcggcgg
     9001 gccgatgggc ctgtcgaccg cctacttcct cggccaggcc ggctaccagg tcaccgtctt
     9061 cgaggcgcgg ccgtacgtcg gcggcatggt ctccggcacg atccccgcgt accgcgcgac
     9121 ggacaaggtg atcgagcaag acctgaacgt catccgccag ctcggtgtcg agttccggtt
     9181 cggccaggag gccggccgcg acttcacgct ggccgatttg cgcaagcagg gctaccgcta
     9241 catcgcgctc ggcgtcggcg cccagaaggg caagaccctg ggcgtcgagg gcgagaccaa
     9301 cgcgggcgtg ctggacgcgc tccagttcct gcgcgactcg cgcgagggac atcccccgcc
     9361 gctcggcaag cgcatcggcg tcatcggcgg cggcgacgtg gccatggact gcgcccgcag
     9421 cgcgtggcgg ctgaccgggg cgaaggtgac catcgtctac cggcgcacga tcgccgagat
     9481 gcccgccgcg cccgaggaga tcgccggcgt gctcgaggag ggcatcgaga tcatggagct
     9541 ggtcaagccc ctccgcgtca tcgcgaagga cggcaagatg accgccctcg aggtccggaa
     9601 aatgaagctg ggcgagaaag acgagtccgg ccggcgccgc ccggtggaca tccccggctc
     9661 cgacttccag ctcccgctcg acaacctgat cgtcgcgatc agccaggagc cctcgctgaa
     9721 gttcctcgag ggcgagccgg tcgagcgcaa caagtccggc tacctcaagg tggaccgggt
     9781 caccatggag acgtccgcgc ccggcctgta cgccggcggg gacgtcgtcg gcgacggccc
     9841 ggaatccatc gtcatggcca tgggcgacgg ccgcaagatc gccgacgcca tccggcgcaa
     9901 ggaagagacg atcgaggaac gccgcgccgc gtggccggcg gtgaacgaga cggagctcat
     9961 ccggcgcaag gcgcgccgcg agtaccgcgt gcacctgccg caccgcgatc cggccgaccg
    10021 gttcaacttc gaggaggtcc agctcacgct ctccgaggag gaggcgaagc gcgaggcctc
    10081 gcgctgcctg gactgccaca cgtactgcag cctttgcgtc agcgtgtgcc cgaaccgcgc
    10141 gatcctgacg tacaaggtca aggccttcga gcggcgcctg gccgacctcg cggcgcgcgg
    10201 cggcaagggc gtcgcctcgc ccggcacgac ggttcgcgtg gaccagccat tccaggtcgc
    10261 cgtcctgacg gacttctgca acgagtgcgg caactgcgag accttctgcc cgaccgcagg
    10321 cgcgccgtac aagatgaagc cgcgactcta cctgcgccgc aaggaattcg acgccgagca
    10381 ggacaatgcc ttcatggtgt tccgcgccaa tgggcacatg ggcatccgcg gccggaaggc
    10441 gggccagacc cacgagttcg tggtcgccgg cgacgagctg cgttacagcg cgccgggctg
    10501 gcgcgtgaaa atggggacgg acttctccgt gcgggaagcg gcggctacag gggcggcgga
    10561 tggtgacgtt ctcggcctcg acttctgcgc gaagatggcc gtcctgctcg acggcctgac
    10621 cacctcgcag cccgcgctgc ccgtggccac ctgagcggga aaaaatctat tcctgcccga
    10681 acaggttcat ctggttcggg tcgcgcaggc gcttgcggcc gccgccgtgg cgagccaact
    10741 tgggctggcc gccctcgccg aactctccct cctcgaggtt ggccaggatc tcccgcgcgc
    10801 gatcgacaac ggccgcgggc agcccggcca ggcggccgac ggcgatcccg tagctcttgt
    10861 ccgccgcgcc gggcacgatc cgccggagga acgcgatctt gtctccgcgc tcgcgcacga
    10921 ggacgttgta attctttacg cccggcatgg ttagcgcaag atcggtcagc tcgtggtagt
    10981 gcgtggcgaa gagggtcttg gccttcacgg gcgcgtggtt gtgcaggtgc tcggcgacgg
    11041 cccacgcgat gctgatgccg tcgaacgtgc tcgtgccccg gccgatctcg tcgagcacga
    11101 tcaggctgcg cggcgtggcg ttgtttagga tgttcgccgt ctcctgcatc tcgaccatga
    11161 acgtgctgcg cccgcgcgcg agatcgtcgc tcgctccgac ccgggtgaac acccggtcca
    11221 cgacgccgag ttctgccgac gcggccggga cgaacgaccc gacctgtgcc atgatcacga
    11281 tcagcgcgac ctggcggatg tacgtggact tgcccgccat gttggggccc gtgatgatga
    11341 tcagttggtt ggccgtccca tccatcacgg tgtcgttcgg cacgaaccgc tcggactcgg
    11401 gcatctgctc gatcaccggg tgacggccct cgcggatctt cagcgcgtcg ccgccgttca
    11461 tcgccggccg gacgtagccc agggccagcg cgcgctcggc gagcgaggac agacaatccg
    11521 cggcggccag cgcctcggcc gactgctgga tcgccgcggt ctccttgacc acggccgcgc
    11581 gcagttcggc gaacagttcc agctccagcg cgagcgcccg ctcctgcgcg ccgaggatct
    11641 tgttctcgta ctccttcaac tgcggcgtga tgaaccgctc ggcgttgacg atcgtttgct
    11701 tgcggatgta gtccgccggg acggcgtcga ggttgctctt ggtgatctcg atgtagtagc
    11761 cgaacacctt gttgtggcgc accttgagcg acttgatgcc ggtgcgctgc tgctcggcgg
    11821 cctggaagtc ggcgagccac tggcggcctt ccgtggcggc ggcccgcaac tcgtccaact
    11881 ccgggtgata gcctttccgg aacaggccgc catccttgat cacggcgggc ggctcatcga
    11941 cgatcgcgcg ggcgatcagg tccacgagtt cgggcagggg gcggatgtgc gcggcgagat
    12001 cgggcagcgg gccggcgggc gcgccggcga gcacgtcgcg caggccgggc agttcgacga
    12061 gggagccgcc gaggccgacc atgtcccgcg cgttgcctgc gccggcgccg agccgggcga
    12121 tcagccgctc caggtcccgg acgcggccca accgctcgcg caggtcggcg aggcgcgggc
    12181 ggtcgcggaa gagggcctcg acggcgtcgt gccgttggac gatttggccg aggtccgtca
    12241 gcgggcgcag cacccattcg cggagcaagc gcgcgcccat cggcgtgcgc gtggaatcga
    12301 ggacgcgcag cagggtggtc gcgacgccgg cgcgggcggg gccgcgggag gcgacgaggt
    12361 cgagattcgc cgccgtggat tcgtcgagga ccatgaactc cgccgggttg cgcaggcgca
    12421 gttgccggat atgcccgacc tggcggcgca gttcgtggac gacgtagtgc aggacggcgc
    12481 cggcggcgcc gacggccgcg ggcctgtcgt cgcagccgaa cccggcgagc gagtggacct
    12541 cgaaatggcg ggccagcagt tccgtggcgg agacgggctc gaaggtccag tcctcgtacg
    12601 gcgtcacgag ggtctgtccg caggcctcga ggacggcgcg gacgcgggga tcatccttcg
    12661 ccgtttccgg gacgacgcat tccgagggcg cggcctgggc cagcggatcg cgcagcgcga
    12721 ccgggtcggc gtgctcctcg atggtgaacg agcccgtgga gaggtcgagc agggcgacgc
    12781 cgatccggtc gccggcgagg ccgacgcccg ccaggtagtt atggcgggcg gactcgagga
    12841 cgttctcctc gaggacggtg ccgggcgtga cgatgcgggt gacctcgcgc tggacgatgc
    12901 cgcgggcgac ggcggggtcc tcgacctggt cgcagatggc gaccttgcgg ccggcgcgga
    12961 tgagcttggc gaggtagagg tccgcggcgt ggtagggcac gccgcacatg gggacgccct
    13021 ggcgcttggt cagggcgatg tcgaggatgg cggaggcgtc cttcgcgtcc tcgaagaaca
    13081 tctcgtagaa atcaccgagc cggaagaaca ggatcgcgtc cggcggcagg tcccgccgga
    13141 tgcgccggta ctgactcatc atgggggtgg cggcatcggt catggtcgcg ctccacgccc
    13201 cggggcgggc ccggcgtacc gggccggatt aatattcccg atccttgccc ccgccgcgga
    13261 ggtcgtcctc cccgttgtcg aacttctctt cctcctcgtc gtcctcaagg acctcgtcga
    13321 ggtcgttgac gtcctcgaaa tcgggatcgg cctcctcctc gtcgtcgtcg gagggctcgg
    13381 cgcagtccgg gcagacggtg gagcgggggt ggcaggcgcg gcagtactcg gccccgcaca
    13441 tgctgcactc gcgcatcttc tcgtccccgt gcgtggggca gccgggttcg acgccgtaaa
    13501 ggttggtttc ttcttcgctg gccacggtgg tctccttcgc gggttgacta gtcctcgccc
    13561 agctccacgt tccagtacgc caggtccagg aaatgccgcc agctgtggtc cgcggtgcgg
    13621 acgaactgga tctcgacgaa cgggcgccag cgcggcttct tgggcttgcg gatcagccgc
    13681 atgccggcct gctcgggcgt ccggttcgcc ttgaggcggt tgcagtccac gcagctgcag
    13741 acgacgttgg tccactgcgt caggcccccg gcctggcggg ggacgacgtg gtcgatgttc
    13801 aggtcgcgcc ggtcgaaccg cttgccgcag tactggcagg tgttccggtc gcgttcgaag
    13861 acgttgtgcc gggtgaactt gacctccttg ttcggcaggc ggtcgaagaa cagcagcagg
    13921 atgatccgcg ggatgcggat gcgcagggag atggtccgga ccgcgcccgc ctcgccggcc
    13981 gtcggggaga agtcgcacca ttcgcggaac gagaacgtcc cgaaaccggt gggcgtctcg
    14041 cacacggcct gcgcgtggcc ggtgtagagc agggcgagcg cgcggtccgc cgtgcagacg
    14101 ttgatcgcct gccagaggcg gttcagcacg agcaccggat gctgcatggc ttcgatggac
    14161 atgggccgcg ttccgttttc gcgttgaata gcacaggcgc cccgcggtgt caaagggctg
    14221 atcgcattga ttttccctgc gcgttttgcc atggtgtccc cgggccggga gcggcccgcc
    14281 atgaaatcgg cgttcctcga caaactgatg gaacggcttg accgggtgga ccccggcagc
    14341 ctgcagacgc acttcctgcg cctcgcccgg gagaaaggcc tgctggagac cctgttccgc
    14401 gccgtgcgcg agggcatcat cgtcctcgac ggccgcgggc gcatcaccta cgccaaccgc
    14461 gcggcggagc agatgctcgg cttccaggcc gacgccgccg cgggccagtc catccgccgc
    14521 ttcctgcgcg agatggactg ggaccggctg ctcgagctcg atcccgagga gtggtcgcgg
    14581 ctcgtctcgc gcgagatcga gatcacctat cccgagcgcc ggctcctgga cttttacgtc
    14641 gtgcccctca ccgccgtcgc cgagagcgag cgcggcgccg tggtcctgct gcgggacgtc
    14701 acctccgacc gcgaccacga ggcccgcgcg gtcgaatcgg agcggctcaa cgccctgacc
    14761 ctgctggccg ccggcgtcgc gcacgagatc ggcaacccgc tgaactcgct gcacatccac
    14821 cttcagttga tcgagcgcga gctgggcggg ctgcccccgg agaagcgcag ggaactggtg
    14881 gacctggtcg aggtcagccg ccgcgaggtc gagcggctcg acggcatcct cacccagttc
    14941 ctccgcgccc tgcggcccgc gccgccgaac ctcgcgcccg gctccgtggc cgacgtcctg
    15001 cgcgagacgc tgaacttcct ccagcacgag atccgcgacc gcgacgtgct cgtcgaggtc
    15061 gaggcccccg aggccctgcc ggcgatcccg atcgatgcgg accagatcaa gcaggcgttc
    15121 ttcaacatca tccgcaacgc catccaggcc atgcccggcg gcggcctgct gaagatcacc
    15181 ctgcgcgaga ccgaccggtt cgtggagatc gccttccggg accgcggccc gggcatcccg
    15241 ccggaggaca tccaccggat cttcgagccc taccagacca cgcggccgga cggctcgggc
    15301 ctcggcctcg ccatcgtcca gcgcatcgtg cgcgaccacg gcggacagat cgaggtccac
    15361 agccggcccg gcgaaggcac gaccttcacc ctcctgctcc cacgcaccgg gcagcgcctg
    15421 cggctgctcc aggcctcgcc ggacctcgag ccggccgcac ccgatggagg cccgccgtga
    15481 aaaagcagcc cgtgatcctg atcgtggacg acgagaaaaa cacccgcgac gggctcgccc
    15541 gcgccctcgg ccggaactac gccgtccgcc tggcggacag cgccgcgcgc gccctgcaga
    15601 tcctggacga gcagcccgcg gacgtgctct tgagcgacat ccgcatgccc ggcgaggacg
    15661 gcctggccct gatgcagcgc gccctcgccc gcaccccggc gcccgtctgc atcctgctca
    15721 ccgcctacgg cagcatcgag acggcggtgg acgcgatgaa gcgcggcgcc tacgacttcc
    15781 tcaccaagcc cgtcagcctc gaccggctcg aactcctcct ccagcgcgcc ctgcgcgagc
    15841 gcgacatgga gagcgagaac cggcgcctgc gcgagcagct cgacgcccgg ttcgggctgg
    15901 agcagctcat cgggcggtcc gcccccatgc aggaggtctt cgactccatc cgccaggtcg
    15961 gcccgtcgcg ggcgacggtg ctggtcaccg gcgagagcgg cacgggcaag gaactggtcg
    16021 cccgtgcgat ccaccagttg agcccgcgcg tccacgggcc gttcatcccg ctccattgcg
    16081 ccgcgctcgc gccgacgctc ctcgagagcg aactgttcgg ccacgagaag ggcgcgttca
    16141 cgggggccgt cgaacggcgc cgcggccggt tcgagctggc ggacggcggg tcgctgttcc
    16201 tcgacgaggt cggcgagatc ccggcggcta tccaggtgaa gatcctgcgc gtgctcgagg
    16261 agcggaagtt cgagcgtgtc ggcggccagg agaccgtcga ggtggacgtt cgcctgatcg
    16321 ccgccaccaa ccgcgacctg aagaagatgg tggacgaggg aacgttccgc gaagacctct
    16381 actaccggct gaacgtcgtc acgatccacc tcccgccgct gcgggagcgg ccggacgaca
    16441 tcccgctgct ggtccagcat tttctcaagg agctctccgc cgagaacggc aagacgatcg
    16501 ccgggctgac gcccgaggcc atggccgcgc tcgccgcgca cacctggccg ggcaacgtgc
    16561 gcgagctgcg gaacgtcatc gaacgcatgg tcgtcatggc gcgcggcgac accctgaccg
    16621 tccgggacat tccggccgcg ctccgcgacg agtcgcgccc gcgcggcggc ctgcccgccg
    16681 ggccggtttc cctcgacgag gcggagaagc agctgatcct ccgcgcgctg aaggcctgtg
    16741 acgacaaccg caccgcggcc gcgcggcagc tcggtatcag ccgccgcacc ctgcaccgga
    16801 agctcaagga gtacgggctg gcggaagacc cgccatcgag cccttgagca acccgccgct
    16861 ccatcggcgg aaatcagggc ttcccttttt caccgccggc tgtatgttgg gccgcttgtg
    16921 aatgcttcga attcaaccgt tattctgggc gcactacgca tccaaatgct ggccccgacg
    16981 ctggtccgta ttgaggaggc tggcccgcgc ggctttgaag accggcccac gttcacagtg
    17041 gtggagcgcg actggccggg ggttcccgtc gagcgtgaaa tccgggatga tacgaccctt
    17101 ctgcgcgcgg gagaggtgcg cgtcgaggtc c
//
LOCUS       DAOOTK010000061        16928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2329_length_16928_cov_4.488710, whole genome shotgun sequence.
ACCESSION   DAOOTK010000061 DAOOTK010000000
VERSION     DAOOTK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16928
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2329_length_16928_cov_4.488710"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..1194
                     /locus_tag="PK634_08205"
     CDS             439..1194
                     /locus_tag="PK634_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97754.1"
                     /translation="MARTLADEGTPAAYLELVRLINNLPDGPERRAALVAATKLDNPA
                     TAALLFETVRVSGDPELVQSAQMSLGGMTNEGVVWLTVALYRGTEDVAQQERFLGVIR
                     HCQQPVFVAPLISVADQSSGDPADPLISAALDTLGLLGTTESIGYLVARLNSAARAGQ
                     DTTALAASLGRVVAPSALADLTGIASGRSPADQMESRVAAARALGHYPLADVEDTLNA
                     ILQNESDPRLINAAQEAMDLATGQVPADFSGTP"
     gene            1223..2023
                     /locus_tag="PK634_08210"
     CDS             1223..2023
                     /locus_tag="PK634_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97755.1"
                     /translation="MKMALKLAACILLLAAGPVARAQVPARIHYQGRLLNGTNLFNGA
                     VPIALQLYSNAAPSAGEAVLYAVTNPSVAVVDGLYALTLGTGTNSGNLPAALARPAVY
                     LQVVVSNTVLLPRERLEAAPYAAALGSTTYIATAQAAVHLGTNGLFGQANAFTPDPFR
                     QPGLFVECSISDDESSGMFLNGNTLVLWSPGDNNDLLRVYDEDDFAGGPRMKLDSDGN
                     LYLTGSVIHTNYISGRTGSESDENADLRRRVAELEARVAELEQRLSDR"
     gene            2188..2427
                     /locus_tag="PK634_08215"
     CDS             2188..2427
                     /locus_tag="PK634_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97756.1"
                     /translation="MIRWLGESSPWTPYLYLYGLGLVTFLIGLRVILKSRACQPGRGH
                     DSAWLAVLLLGYAFFALLHAAWIMAALYIPFKGGG"
     gene            2429..4327
                     /locus_tag="PK634_08220"
     CDS             2429..4327
                     /locus_tag="PK634_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOW97757.1"
                     /translation="MHHGFGIDITTGATIVVAIVSFIYFIGIMVFGTYFSKFSKNLND
                     YFYSGQRFAWWLAAASMVATGIGSYSYLKYSEQGFNTGMSSTMTYMNDWFIVPFFIFG
                     WLPIIYFARVRSIPEYFERRFNRTARYFAVIIIMAYMFFYIGYNLFTMGVAIEGMFGI
                     RQLWSIPVITLFIGIYVTFGGQTAVIFTDLVQGIMLYIAGGLAIVAGLWALGGFGEFW
                     GWLPVAHRLPFVDLVADPKFNASGLFWGEALAGSIAFTFMNQGFIMRYLTIRSVHEGR
                     KAALFNVVVCLPLSAIVVGAVGWLARAIVTKQAAVGGALAGIEPIHIENTYHTFIVVC
                     WETLKRNSWLFGFVVAALTAALMSTIDTLINACAAIGIYDIYKPLIKPHADDRHYLKA
                     ARWASALATVLGLLLVIWFNRQTGSLMAIHYKGIMLIIPPIVTTIFLGAFWKRFNALG
                     ACVSMLIGVVFTMATNWWPGLVRPLAWFVVGNAADIYMPALFGMLVTAVAGVVVSLLT
                     RPDTHKDITGLTVETLDKAMEMYKGGPPNHVPGRPVKRIEVRLDESVPPGLISVSPAA
                     METMKAHEGDMVYMTDARPWLGGLRSQHVRFAAPHAQADRVVLMSKATRDDAFLLDGR
                     PVTLDKIF"
     gene            complement(4469..5878)
                     /locus_tag="PK634_08225"
     CDS             complement(4469..5878)
                     /locus_tag="PK634_08225"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOW97758.1"
                     /translation="MRARDTFDVGCGILAGVADGMAFFAGFLLAVWIRFDSGWIPLIG
                     EGLPPRMMYVYGGGVATLLCLFIFRSLELYRRPQSGRFEDKIPRIVRASGWAILLATA
                     LAFIIRTDPPFSRAVTILAFFTATFLVLLERYLLFRLELHWARHREEINRVIVIGTDA
                     VAAHLKEALKKEPRLRCRVTGFLTTGEDPSDPAIPPDLILGGAEDLEKFIERGEVDQV
                     ILADTSLSHRRMTEIILHCERGLVHFQLVPDLFAVLTSRVQVQHVGDVPLLSVRKWPL
                     DYFWNRAFKRSEDILGSLVGLVISAPIMAVAAVAIRRSSPGPIFYRQVRCGEGGKCFT
                     LYKLRTMREDAEEESGPVWTSENDPRRTRAGAFLRRWNLDELPQFWNVLKGDMSLVGP
                     RPERPVFVEQFKEDVGRYMWRHVHKPGLTGWAQVNGLRGNTSIQDRIQYDLFYLENWS
                     LALDFKILLMTFVARKNAY"
     gene            5944..6486
                     /locus_tag="PK634_08230"
     CDS             5944..6486
                     /locus_tag="PK634_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOW97759.1"
                     /translation="MTRVLVPLAEGCEEMEAVIIIDVLRRAGWAVTGVGLQPGPVRGS
                     RGVILLPDAEWDETNWAEFDLLVLPGGGPGTQRLAQDARVLAAVKGFNDAGKRLAAVC
                     AAPLVLQQAGVLDGRKATCHPSLASRLTRARYMTEPVVVDGRITTSQSAGTCLAFALE
                     LIRQVDGDGKVREVREGLAM"
     gene            6604..6882
                     /locus_tag="PK634_08235"
     CDS             6604..6882
                     /locus_tag="PK634_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOW97760.1"
                     /translation="MALTKSAIQAALAGKTGLAKKQVAAFFDELAKLAYKEAKNSFTI
                     PGIGKLVLVNRAARIGRNPATGEQIQIPAKKVVKFRVAKAAKVAILGK"
     gene            7037..8227
                     /gene="ygeW"
                     /locus_tag="PK634_08240"
     CDS             7037..8227
                     /gene="ygeW"
                     /locus_tag="PK634_08240"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOW97761.1"
                     /translation="MSDVSKQIEIIRRLNASLFGKDFLLTWDKSIAELELIMAVAEAL
                     RGLYRQNGSARCFDSGLAVSLFRDQSTRTRFSFASASNLLGLTVQDLDEGKSQIAHGE
                     TVRETANMISFLTEAIGIRDDMFLGAGHTYMLEVAAALDDGFKSGVLHQRPAVVNLQC
                     DVDHPTQAMADLMWLKEYFGSLENLRGRKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MHVSLAHPEGYDLIPDVVELARKNAGATGGTFTTGASMDEAFRNADIVYPKSWAPFRV
                     MQRRTELLRGNDRDGLKQLEKECLANNARFTGWECTEQKMKLTKNGAAIYLHCLPADI
                     SGVSCKQGEVQDSVFDRYRIQTYKEAGWKPFAIAAMILTSRFKDPAGVLEKLAKGGRP
                     RLIG"
     gene            8237..9181
                     /gene="arcC"
                     /locus_tag="PK634_08245"
     CDS             8237..9181
                     /gene="arcC"
                     /locus_tag="PK634_08245"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOW97762.1"
                     /translation="MPKPLVVIAIGGNSLIKDAKHMTVLDQYLAAGETSHHIAALVNE
                     GYRVAITHGNGPQVGFILMRSEIARTQLHQVPLESCVADTQGAIGYQVEQTLMNELHR
                     QGVRHSIVSVVTQVVVDKDDTAFQNPTKPIGPFFTKEDAFQHQEKDGWAVKEDAGRGW
                     RRVVPSPKPLEIVEEGVIRTLLENDVIVVAVGGGGIPVIRLPDGDLQGCAAVIDKDAA
                     SSLLARHLKADLFVISTGVDKVALDFGKPTQRYLDRMTLAEARRHLKDGQFPAGSMGP
                     KIEAAISYLEDGGREVIITQPHLLEDAIRGKTGTRIVP"
     gene            9195..10463
                     /gene="thrC"
                     /locus_tag="PK634_08250"
     CDS             9195..10463
                     /gene="thrC"
                     /locus_tag="PK634_08250"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOW97763.1"
                     /translation="MFFTGFECIACAATQPSSFKGFTCPACGGNLTVTYDYDGLKNRV
                     GKERLVTFGRPDLFRYAPLLPLERPGLASPLRIGWTPLYPAPRLGTPLGLKHLYLKDD
                     GLNPSASFKDRAGAVALVRARETGARVIAGASTGNAGSSMACLAASVGMPCVIFVPEK
                     APPAKIAQLLVFGARVMAVRGTYDDAFDLCMKVCAERGWFNRNTGHNPFTREGKKTCS
                     FEICEQLGWHAPDRVVVATGDGNILSGIWKGMKDLHAVGLIDRLPKIDCAQSEKSDAV
                     TRAVQNVRQDHPRGAAVDWKRVAVEPVKATTLADSISVDAPRDGLAAVRAVIESGGEV
                     CTVPDEEILSAIPELARGAGVFAEPAAACAWAVTRRLAMSGRIQPEEKVVCLITGNGL
                     KDAGAARKAAGEPTLIKPTVEAALAVLADL"
     gene            complement(10458..11498)
                     /locus_tag="PK634_08255"
     CDS             complement(10458..11498)
                     /locus_tag="PK634_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97764.1"
                     /translation="MNGLPHRSPAVRGSRRAAAALLLLPGLLGPGCSTYHRYRARFME
                     PDYLVTREFFTEAPRHVALLPFAARSGRARDAANAQVCRRAFYQHLSLRSFEETDLRD
                     VDRELLDPKDPETRTRVRKFADALRRLDVLGMSSLLDMELLFRENEALYREHYRRLVD
                     DIVRKELKADSYVVGISRSYGRLYLVVASSIALSTRLELRSVKDDALLWRAQAQRRNT
                     AFPLTLNPFDIPFLLVDIWKNSKGGALDLLAYEVFGSVTATIPPVSKSVEPYIEALQP
                     RTLVYRAPTLWRFRRSGWVDGGTRWPLLLEKRGWFECRGQDGQSFWIFRRDGRRVDRK
                     GKPIIEPPVPAL"
     gene            11610..12032
                     /locus_tag="PK634_08260"
     CDS             11610..12032
                     /locus_tag="PK634_08260"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOW97765.1"
                     /translation="MNRQQILDFANRVQTCHLATVENGEPRVRGMMMYRADESGVLFH
                     TGTGKDVFREIQANPAVEICFNDLQAGIQVRIRGRAQVVEDQALKEEIVERRDFMKPW
                     IKEHGYGLLGVFRVTDCRASVWTFAANFAPKEWVRLSS"
     gene            12029..13690
                     /locus_tag="PK634_08265"
     CDS             12029..13690
                     /locus_tag="PK634_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97766.1"
                     /translation="MTSASLPAASLNLLGVDYLHVRTGDQGDLYLTRHGAPFREHLLP
                     ENWYDMAWFERNREKLIGTGTVYRVRTRPIRGCSKDLVVKWCRVGEEVPMDTFTLSKF
                     VEAEFNSPYEEFSLVMEMRARSTQGMVRTHKPLGIYVPAKKLKLWQTGRSLSRMDQKK
                     AKYRDVELDIYRQYILIYEWIKGVNATEAFERILPDARQRRQELIAFTDRVTNDLHRK
                     GFRVLDTKPEHFIVRPRPDGSLLRTPGGEIAYALVDFELLERTPEREQEVTASRRAAY
                     LQHQKNRFNDSAGKTFPGHLTPCRVMGVDYVYGHSESTQGRLWIVGCDPSLFDYFLPE
                     RWRRTPRRALSKTAQTFYTRSKDLINLVWKVSRVGERPDPEASSEARVRITEHGYNSP
                     FEEFALALELGRRGLATTYPRAIYMTGQEAAPDAYTLDPSRYVSHRGILTPAGDPALR
                     TDHNYITVWGFWNGSDELLATRDVEYCRGVDLLRALEDGLIDADEFTALLDRARAELA
                     HAGFEDLNLKGTHVMLSMNPQGHLIRQESGSPALRFSNFSFLRTK"
     gene            complement(13846..14640)
                     /locus_tag="PK634_08270"
     CDS             complement(13846..14640)
                     /locus_tag="PK634_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOW97767.1"
                     /translation="MKRKIITIDEALCNGCGACVTACAEGALRIVDGKARLVREQYCD
                     GFGDCVGECPTGALKIVEAEAAEYDEAATRRHVAQSGGAAAVRAFDAAASRHEPREHT
                     ARPGGCPGMAVRDFPEGRRPASAVAGHAGLPPQVNASELRQWPVQMHLVPPGAPFFKG
                     RELVIMSTCAPLASADMHWRFLRGRSVVVGCPKLDRTEGYVEKLASILSEPSIPRVLV
                     VRMEVPCCGKLTNLVQEAVRNTGRGDLSMDEVIVSLEGNVTQGHGG"
     gene            complement(14642..16294)
                     /gene="hcp"
                     /locus_tag="PK634_08275"
                     /gene_synonym="priS"
     CDS             complement(14642..16294)
                     /gene="hcp"
                     /locus_tag="PK634_08275"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOW97768.1"
                     /translation="MFCYQCEQTAKGTGCTSFGVCGKDPDTAALLDLLVHAQKGISQY
                     AHRARQLGARDHEVDVFALESLFTTVTNVNFDPRRIEVMLRRAALLKDRARALYEKAC
                     AAGGVAAEKLSGPAAWQPAADLDGLIRQGEQVSVEGRMAVRGADITGLQELLMYGLKG
                     MAAYADHALILGKEDESVYAFMHEALDFLTQEAPTVDQLLGMNLRCGEVNLTVMGLLD
                     AANTGAYGHPVPTPVRITPVKGKAILVSGHDLKDLEELLKQTEGTGVHVYTHGEMLPA
                     HGYPGLKKYRHLVGNYGGAWQDQRKEFDAFPGAILMTTNCIQKPKESYAGRLFTSGLV
                     AWPGVRHLARRDFGPLIEAARSAPGFAADEPEKTILVGFGHHAVLGVADQVIGAVKKG
                     DIRHFFLIGGCDGAKPGRDYYTEFAQAVPKDCVILTLACGKYRFNKLDFGTVAGLPRL
                     LDVGQCNDAYSAIKIASALAGAFGCGVNDLPLSLILSWYEQKAVCILLTLLHLGIRNI
                     RLGPSLPAFLTPPVIKVLVEKFGIQPVGAVEKDLATALGSAA"
     gene            16505..>16928
                     /locus_tag="PK634_08280"
     CDS             16505..>16928
                     /locus_tag="PK634_08280"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOW97769.1"
                     /translation="MNSRALLLVLALGAAGPGCALVRSGVGRAMAPMAEDLAASLQQQ
                     SDVELVREGAPAYLLLLDGLADSGSRQPRILLAAAEANTSYAMAFLDQADRERAKAFH
                     ARARDYGLDVLRRNRKFRAAERAPLMDFVYPVVPGQFTT"
BASE COUNT         2909 a         5793 c         5450 g         2776 t
ORIGIN      
        1 cgacagccgc ggcgccatca ccgagtccag cgaaggcaac aaccagtcca ccaagtccta
       61 cacctactga atggaaaagg gcagatgatc gcggcgcgtc ggcttcgttt tcgtgacgct
      121 gtggcgctgt tggcgctcgc agtcgttttt tcctctgccg gctgccgccg ggcggaagcg
      181 ccgccgcagg aagtccaggt tcccgccgaa agctcaacgc catcccgttc cggatcccat
      241 cccgggtcga cacccgaggc gcccccccca cccttctccg ctgcggcgcc ggatggccgg
      301 acggccgcgc aaaatcccgc gacagccgga gaatccggcc ctgccgcggg gaacgccgcg
      361 acgcccggcg ccccgacagg ctccggggct ggccgcgcgc ccccggcgac ctacgatccg
      421 gccatcagtc ccacggccgt ggcgcgcacg ctcgcggacg agggcacccc ggcggcctac
      481 ctggagctcg tccgcctcat caacaacctg cccgacgggc ccgagcgccg ggccgccctg
      541 gtggccgcca cgaagctgga taacccggca accgcggcgc tcctcttcga aacggtgcgc
      601 gtgtcgggcg atccggagct cgtccagtcc gcccagatga gcctgggcgg aatgaccaac
      661 gaaggcgtcg tgtggctgac cgtggccctg taccggggca cggaggatgt cgcgcagcag
      721 gagaggttcc tcggcgtgat tcgccattgc cagcaaccgg tctttgtcgc gccgctgatc
      781 tccgtagccg accagtcttc gggcgacccg gcggacccgc tgatctcggc ggcgctggat
      841 acgctcgggt tgctcggcac gaccgaatcc atcggatacc tggtggcccg cctgaactcg
      901 gcggcccgcg cgggacagga caccaccgcg ctcgccgcct ccctgggccg ggtcgttgcg
      961 ccctcggcgc tggccgactt gacgggcatc gcgtccggcc gatctccggc ggaccagatg
     1021 gagtcccggg tcgcggccgc ccgcgcgctg ggccactatc cgctggccga cgtggaggac
     1081 accctgaacg ccatccttca gaacgaaagc gacccgcgcc tcatcaatgc cgcgcaggag
     1141 gccatggatc tcgcgacggg ccaggtcccg gcggattttt cgggaacccc ctgatcgcgg
     1201 gccgccaaga aaaggaaggg tcatgaagat ggctttgaaa ctcgccgcgt gcatcctgct
     1261 cctggcggcg gggcccgtcg cccgggcgca ggtgcccgcg cgcatccact accaggggcg
     1321 gctgctcaac ggcacgaacc ttttcaacgg cgccgtgccg atcgccctcc agctatactc
     1381 caacgccgcg ccttccgcgg gagaagccgt gctgtacgcc gtcaccaatc cgtccgtggc
     1441 cgtggtcgat ggcctgtacg ccctgacgct cgggaccggc acgaattccg gcaacctgcc
     1501 ggccgccctg gcgaggcccg ccgtgtacct gcaggtcgtc gtcagcaaca ccgtgctgct
     1561 gccccgcgaa cgcctcgagg ccgcccccta cgctgccgcg ctcgggagca ccacctatat
     1621 cgccaccgcg caggcggccg tccacctggg caccaacggc ctgttcgggc aggcgaacgc
     1681 cttcaccccg gatcccttca ggcagcccgg cctgttcgtc gaatgctcga tctccgacga
     1741 cgaaagctcc gggatgttcc tgaacggcaa tacgctggtg ctctggtcgc ccggcgacaa
     1801 caacgacctg ctgcgggtct acgacgaaga cgacttcgcc ggcggcccgc ggatgaagct
     1861 cgacagcgac gggaatctgt accttaccgg gtccgtgatc cacaccaact atatatccgg
     1921 ccggacgggt tccgaatcgg atgaaaacgc ggacctccgc cggcgcgtgg ccgagctgga
     1981 agcccgcgtg gccgagttgg agcagcgcct ttcggatcga taaggcgcgc ggcttgttcc
     2041 tccgggcgac ggccgcgggc tcaacttgat tgacaagaag cggcgcggca tggaagatga
     2101 actccagatg atatgagttc ccggaagtca gaggccccgc tcattctcgc cggcacgctg
     2161 tttttcgttt atgggtttcc acgcgccgtg atccgatggc tgggcgagtc gagcccttgg
     2221 acgccgtacc tgtacctgta cggcctcggg ctggtgacgt tcctcatcgg gctgcgcgtg
     2281 atcctgaaat cgcgggcctg ccagccgggc cgcggccatg actcggcgtg gctggccgtg
     2341 ctcctgctcg gctacgcctt cttcgcgctg ctccacgcgg cctggatcat ggcggcgctg
     2401 tacattccgt tcaagggagg cgggtgacgt gcaccacggc ttcggcatcg acatcaccac
     2461 gggcgcgacg atcgtcgtgg cgatcgtgtc cttcatctac ttcatcggca tcatggtctt
     2521 cgggacctac ttctcgaagt tcagcaagaa cctgaacgac tacttctaca gcggccagcg
     2581 cttcgcctgg tggctcgcgg cggcgagcat ggtggccacc gggatcgggt cctacagtta
     2641 cctgaagtac tccgagcagg gcttcaacac cggcatgagc agcacgatga cgtacatgaa
     2701 cgactggttc atcgtgccgt tcttcatctt cggctggctg cccatcatct acttcgcgcg
     2761 ggtgcgctcg atcccggagt acttcgagcg gcggttcaac cggaccgccc gttacttcgc
     2821 cgtgatcatc atcatggcct acatgttctt ctacatcggc tacaacctgt tcacgatggg
     2881 cgtggccatc gagggcatgt tcggcatccg ccagttgtgg tcgatccccg ttatcacatt
     2941 attcataggc atttacgtca cgttcggcgg ccagacggcc gtgatcttca cggacctggt
     3001 gcaggggatc atgctgtaca tcgccggcgg cctggccatt gtcgcggggt tgtgggcgct
     3061 gggcggattc ggcgaattct ggggctggct gccggtcgcg caccggctgc cgttcgtgga
     3121 cctggtggcg gatcccaagt tcaatgcgtc gggcctgttc tggggcgagg cgctcgcggg
     3181 cagcatcgcg ttcaccttca tgaaccaggg tttcatcatg cggtacctca cgatccgcag
     3241 cgtacatgag ggacgtaaag cggcgctctt caacgtcgtg gtctgcctcc ccctctcggc
     3301 catcgtggtg ggcgccgtgg gctggctggc ccgggccatc gtgaccaagc aggcggcggt
     3361 cggcggcgcg ctggcaggca tcgagccgat ccatatcgag aacacctacc acacgttcat
     3421 cgtggtctgc tgggaaacgc tgaaacggaa ctcgtggctc ttcgggttcg tcgtcgccgc
     3481 gctcaccgcc gcgctgatgt ccaccatcga cacgctcatc aacgcctgcg ccgcgatcgg
     3541 catctacgac atctacaagc cgctgatcaa gccgcacgcc gacgaccggc actacctcaa
     3601 ggccgcgcgc tgggcctcgg ccctcgccac ggtcctgggg ctgctcctcg tgatctggtt
     3661 caaccggcag accggcagcc tgatggcgat ccactacaag ggcatcatgc tcatcatccc
     3721 gcccatcgtg accacgatct tcctcggcgc cttctggaag cgcttcaatg ccctcggggc
     3781 ctgcgtgtcc atgctgatcg gcgtcgtctt caccatggcc accaactggt ggcccggcct
     3841 ggtgcggccg ctggcctggt tcgtggtcgg caacgccgcg gacatctaca tgcccgcgct
     3901 cttcggcatg ctggtcaccg ccgtggccgg cgtcgtcgtc agcctgctga cgcggcccga
     3961 tacgcacaag gacatcaccg gcctgacggt ggagacgctg gacaaggcca tggagatgta
     4021 caagggcggc ccgcccaacc atgtccccgg ccggccggtc aagcggatcg aggtccggct
     4081 cgacgagtcc gtgccgcccg gcctgatctc ggtctcgccg gccgccatgg agacgatgaa
     4141 agcccacgaa ggcgacatgg tctacatgac cgacgcccgc ccgtggctgg gcggcctgcg
     4201 ttcccagcat gtccggttcg ccgcgccgca tgcgcaggcg gaccgcgtcg tactgatgtc
     4261 gaaggccacg cgggacgacg ccttcctcct cgacggacgg ccggtcacgc tggacaagat
     4321 attctgatgc ctcgggcgcc gtcgccgatg gatgagccgc ccgctccagg caggggaaca
     4381 acggctggcg ccggcccttt tccgcaggta tcacagttta taaactgtga tacctgcctt
     4441 tccggctgtt ccccggcccc gggcaggctc aataagcgtt tttccgggcc acgaaggtca
     4501 tgagcaggat cttgaagtcg agggccaggg accagttttc caggtagaac aggtcgtact
     4561 ggatgcgatc ctggatgctc gtgttgccgc gcagcccgtt gacctgcgcc caacccgtca
     4621 gccccggctt gtgcacgtga cgccacatgt agcggccgac gtcctccttg aactgctcca
     4681 cgaacaccgg ccgctcgggc cgcggcccga caaggctcat gtcgcccttc agcacgttcc
     4741 agaactgcgg cagctcgtcc aggttccagc gccgcaggaa cgccccggcc cgggtccggc
     4801 gcggatcgtt ctcgctcgtc cacaccgggc ccgactcctc ctccgcgtct tcccgcatcg
     4861 tgcgcagctt gtagagcgtg aagcactttc cgccctcgcc gcagcgcacc tgccgataga
     4921 atatcgggcc cggggacgag cgccggatcg ccaccgccgc gaccgccatg atcggcgcgg
     4981 aaatcaccag ccccaccagg ctccccagga tgtcctcgct gcgcttgaag gcccggttcc
     5041 agaagtagtc cagcggccac ttgcgcacgc tcaacagcgg cacatcgccc acgtgctgga
     5101 cctgcacgcg gctcgtcagc acggcaaaca ggtcgggcac cagctggaaa tgaaccagcc
     5161 cccgctcgca gtgcaggatg atctccgtca tgcgccggtg cgacagtgac gtatcggcga
     5221 ggatcacctg gtccacctcg ccccgctcga tgaacttttc gaggtcctcc gcgccgccca
     5281 ggatgaggtc gggcgggatg gccgggtcgg aagggtcctc gccggtcgtc aggaaacccg
     5341 tgacccggca ccgcagccgc ggctccttct tcagcgcctc cttgagatgg gccgccacgg
     5401 catccgtgcc gatcacgatg acgcggttga tctcctcgcg gtggcgcgcc cagtggagct
     5461 ccaggcggaa caagaggtac cgctccagga gcacgagaaa ggtggccgtg aaaaaggcca
     5521 ggatcgtcac ggcgcgagag aacggcgggt ccgtgcggat gatgaaggcc agggccgtgg
     5581 ccaggaggat ggcccaaccg gaggcccgga cgatccgcgg gatcttgtcc tcgaagcgcc
     5641 cggactgcgg ccggcggtag agctccagcg aacggaaaat gaacaggcag aggagcgtcg
     5701 ccacgccccc gccatagacg tacatcatgc ggggcggcag cccctcgccg atcagcggga
     5761 tccagccgct gtcgaaccgg atccacacgg ccagaaggaa gccggcgaaa aaagccatcc
     5821 cgtcggccac tccggccagg atgccgcacc cgacatcgaa ggtatctctc gcgcgcatgg
     5881 aaagacggtt tctttgttgg ttagtaggcg ctttctacca taataagggc aaggagaaaa
     5941 gctatgacaa gggttctggt tccgctggcg gaaggctgcg aggagatgga ggccgtgata
     6001 atcattgatg tcctccggcg ggccggctgg gcggttacgg gcgtggggct ccagccgggg
     6061 ccggtccgcg gctcccgcgg cgtaatcctg ctcccggacg cggaatggga cgaaacgaat
     6121 tgggcggaat ttgatctgct ggtgctgccg ggcggagggc cgggcaccca gcggctggcg
     6181 caggacgcga gggtgctggc ggcggtaaag gggttcaacg acgccggcaa gcgtctcgcg
     6241 gcggtatgcg ccgctcccct ggtcctgcag caagccggcg tcctggacgg ccggaaggcc
     6301 acgtgccatc ccagcctcgc ctcccggctg acccgagccc gttacatgac ggagcccgtg
     6361 gtcgtggacg gacgcatcac gaccagccag tccgccggca cctgcctcgc ctttgccctc
     6421 gaactgatcc gccaggtcga cggcgacggg aaggtccgcg aagtgcgcga ggggttggcg
     6481 atgtaaaggg aaacttgtct cgaaaaaatc gaaaaaaacg cttgttttcc ggccgaagcg
     6541 gggcacaatg caccgcgaat atgtagcagt agcagaacaa caacatcaac gaaggagaca
     6601 gcgatggcat tgacgaaatc ggcgattcag gcggcgttgg cggggaagac gggcctggcc
     6661 aagaagcagg tggcggcgtt cttcgacgag ctggcgaagc tggcgtacaa ggaagcgaag
     6721 aactcgttca cgattcccgg catcgggaag ctcgtgctgg tcaaccgcgc cgctcgcatc
     6781 ggccgcaacc cggccacggg cgagcagatc cagatcccgg cgaagaaggt cgtgaagttc
     6841 cgcgtcgcca aggcggccaa ggtggccatt ctcggcaagt aagcgtcgac gaacagcgca
     6901 ttgaaagcgg aagagatggc gggcctcccg ccatctcttc tgtttttagg gggcttgaac
     6961 tggccctgtg ttttgctacc ataacccacc gcatgagaca gccggactcc cgccccggca
     7021 tggaacagga gaacacatga gcgacgtttc caagcagatc gaaatcatcc gccgcctgaa
     7081 cgccagcctc ttcggcaagg acttcctgct gacgtgggac aagtccatcg ccgaactgga
     7141 gctgatcatg gccgtggccg aggccctgcg cggcctgtat cgccagaacg gctcggcccg
     7201 ctgtttcgat tcgggcctgg ccgtctccct cttccgcgac cagtccacgc gcacgcgctt
     7261 ttccttcgct tccgcgtcga acctgctcgg cctgacggtg caggacctcg acgagggcaa
     7321 gtcgcagatc gcgcacggcg agaccgtccg cgaaacggcc aacatgatct cgttcctcac
     7381 ggaggccatc ggcatccgcg acgacatgtt cctcggcgcc ggccacacct acatgctcga
     7441 ggtggccgcc gcgctggacg acggcttcaa gagcggcgtc ctccaccagc ggccggccgt
     7501 cgtcaacctc cagtgcgacg tggatcatcc cacgcaggcc atggcggacc tgatgtggct
     7561 caaggaatac ttcggctcgc tcgagaacct gcgcgggcgc aagatcgcga tgacctgggc
     7621 ctactcgccg agctatggca agccgctctc cgtgccgcag ggcatcatcg gcctgatgac
     7681 ccggttcggc atgcatgtgt cgctggccca tcccgagggc tatgacctga tcccggacgt
     7741 cgtggagctg gcccgcaaga acgccggcgc gaccggcggg acgttcacga cgggcgcctc
     7801 gatggacgag gccttccgca acgcggacat cgtctacccg aagtcctggg cgccgttccg
     7861 cgtcatgcag cgccgcaccg agctgctccg cggcaacgac cgcgacggcc tgaagcagct
     7921 cgagaaggaa tgcctggcca acaacgccag gttcaccggc tgggaatgca cggagcagaa
     7981 gatgaagctg accaagaacg gcgcggcgat ctacctgcac tgcctgccgg ccgatatctc
     8041 cggggtctcc tgcaagcagg gcgaggtgca ggacagcgtc ttcgaccgct accgcatcca
     8101 gacctacaag gaggccggct ggaagccgtt cgccatcgcg gcgatgatcc tgacctcccg
     8161 cttcaaggac ccggcgggcg tgctggagaa actggcgaag ggcggacgcc cccgcctcat
     8221 cgggtaggac ggcgccatgc ccaaacccct ggttgtcatc gccatcggcg gcaactcgct
     8281 gatcaaggac gccaagcaca tgacggtgct ggatcagtac ctcgccgccg gcgagaccag
     8341 ccaccacatc gccgccctgg tgaacgaggg ctaccgcgtg gccatcaccc acggcaacgg
     8401 accgcaggtg ggtttcatcc tgatgcgctc ggagatcgca aggacccagt tgcaccaggt
     8461 ccccctcgaa agctgcgtgg cggacacgca gggggccatc gggtaccagg tcgagcagac
     8521 gctgatgaat gaactccatc gccagggcgt gcgtcattcc atcgtgtccg tcgtgaccca
     8581 ggtggtggtg gacaaggacg acacggcttt ccagaatccg acgaagccaa tcggcccctt
     8641 cttcacgaaa gaggacgcct tccagcacca ggagaaagac ggttgggccg tgaaggagga
     8701 cgccgggcgc ggttggcggc gcgtggtgcc ctcgcccaag cccctggaga tcgtcgagga
     8761 aggcgtcatc cgcacgctgc tggagaacga cgtgatcgtg gtggccgtgg gcggcggggg
     8821 gatccccgtg atccgtctcc cggacggcga cctgcagggc tgcgcggcgg tgatcgataa
     8881 ggacgccgca tcgagcctcc tcgcgcgcca tctcaaggcc gacctgttcg tgatctccac
     8941 cggcgtggac aaggtggccc tcgacttcgg caagccgacc cagcgctacc tcgaccgcat
     9001 gaccctggcc gaggcccggc ggcatctcaa ggacggccag ttcccggcgg gcagcatggg
     9061 cccgaaaatc gaggcggcca tctcctacct ggaagacggc gggcgcgagg tcatcatcac
     9121 ccagccgcac cttctcgaag acgccatccg cggcaaaacc ggcacgcgaa tcgtgccctg
     9181 aacggagggg gcccatgttc ttcaccggtt tcgaatgcat cgcctgcgcg gcgacgcagc
     9241 cgtcgagctt caagggcttt acctgccccg cctgcggcgg caacctgacc gtcacctatg
     9301 actacgacgg cctgaagaat cgcgtcggga aagagcgcct agtcacgttc ggccggccgg
     9361 acctgttccg ctatgccccc ctgctccccc tggagcgccc ggggctggcg tcgcccctgc
     9421 gcatcggctg gaccccccta tacccggcgc cccgcctggg aacgcccctg ggcctgaagc
     9481 atctgtatct caaggacgac ggcctcaacc cgagcgcgtc gttcaaggac cgcgcgggcg
     9541 cggtggcctt ggtccgggcg cgcgagaccg gcgccagggt gatcgccggg gccagcacgg
     9601 gcaacgccgg cagttccatg gcctgcctcg ccgccagcgt cggcatgccc tgcgtgatct
     9661 tcgtgcccga gaaggcgcca ccggccaaga tagcccagtt gctggtcttc ggggcgcgcg
     9721 tgatggccgt ccgcggcacc tacgacgacg cgttcgatct ctgcatgaag gtgtgcgccg
     9781 agcgcggctg gttcaaccgc aacaccgggc acaatccttt cacccgggag ggcaagaaga
     9841 cgtgcagctt cgagatctgc gagcagctcg gctggcacgc ccccgaccgc gtcgtggtgg
     9901 ccacgggcga tgggaacatc cttagcggca tctggaaggg catgaaggac ctgcatgccg
     9961 tcgggctcat cgatcgcctc ccgaagatcg attgcgccca gtccgagaaa agcgacgcgg
    10021 tcacccgggc cgtccagaac gtgcggcagg atcatccccg cggcgctgcc gtggactgga
    10081 aacgggtcgc ggtggaaccg gtcaaggcca cgaccctcgc cgattccatc agcgtggacg
    10141 cgccgcggga cgggctggcg gccgtgcgcg ccgtgatcga gtccggcggc gaggtctgca
    10201 ccgtgcccga cgaggagatc ctctccgcca ttccggaact ggcgcgcggc gccggcgtct
    10261 tcgcggagcc cgccgccgcc tgcgcctggg cggtcacgcg ccgcctggcg atgtccggcc
    10321 ggatccagcc cgaggaaaaa gtggtctgcc tgatcaccgg gaacggcctg aaggacgccg
    10381 gcgcggcgcg caaggccgcc ggagagccga cgctgatcaa gccgaccgtg gaagcggcgt
    10441 tggcggtcct ggctgattta tagcgcgggg acgggcggct cgataatcgg ttttcctttc
    10501 cggtccacgc gccgtccgtc gcggcggaaa atccagaagg actgtccgtc ttggccccgg
    10561 cactcgaacc agccccgttt ttccaggagc aggggccacc gcgtcccccc gtccacccag
    10621 ccggaccgcc ggaaacgcca gagggtcggc gcgcggtata ccagcgtgcg gggctggagc
    10681 gcctcgatgt acggctcgac cgacttgctc acgggaggga tggtcgcggt taccgagccg
    10741 aacacctcat acgccagcaa atccagcgcc ccgcccttcg agttcttcca gatgtccacc
    10801 aggaggaagg ggatatcgaa cggattcagc gtgaggggga acgctgtgtt ccggcgctgg
    10861 gcctgggccc tccacaggag ggcgtcgtcc ttcaccgatc ggagttcgag ccgcgtggag
    10921 agggcgatgg aagacgcgac caccaggtac agccgcccgt agctccggct gatgccgacg
    10981 acgtaggagt ccgccttgag ctccttgcgc acgatgtcgt cgacgagccg gcggtagtgc
    11041 tcccggtaca gggcctcgtt ttcgcggaag agcaactcca tgtccagcag gctgctcatg
    11101 cccaggacat ccagcctgcg cagcgcgtcg gcaaacttgc gcacgcgggt tcgggtctcg
    11161 ggatccttcg ggtccagcaa ttcgcgatcc acgtcgcgca ggtccgtctc ctcgaaggag
    11221 cgcaggctca agtgctggta gaaagcccgt cggcagacct gggcgttggc ggcgtcgcgc
    11281 gcccggcccg agcgcgcggc aaaggggagc agcgccacgt ggcggggcgc ctcggtgaag
    11341 aactcgcgcg tcaccagata atccggctcc atgaagcggg cccggtaccg atggtaggtc
    11401 gagcaaccgg ggccaagcag gccgggcagg agcagcagcg ccgccgcggc gcgccgcgat
    11461 ccccgcacgg cagggctccg gtgtggcagg ccgttcatgt cgctgccgcc agtttccccg
    11521 cggggacggc cggccgcaag ctcaaacagg ccgctttcgt tctggaacgc gggcccgccc
    11581 tctggcaaag tgaacggcgg gagaacctca tgaatcgcca acagattttg gactttgcaa
    11641 accgggtgca gacttgccat ctggccaccg tggagaacgg cgagccccgc gtgcggggca
    11701 tgatgatgta ccgggcggac gaatccggcg tgctcttcca caccggcacg ggcaaggatg
    11761 tgttccggga aatccaggct aatcccgccg tggagatctg cttcaacgac ctgcaggccg
    11821 ggatccaggt gcgcatccgg gggcgggcgc aggtggtcga agaccaggcg ctcaaggagg
    11881 agatcgtcga gcgccgcgac ttcatgaagc cgtggatcaa ggaacacgga tatgggctgc
    11941 tcggcgtctt ccgcgtgacg gactgccggg cttccgtgtg gaccttcgcc gccaacttcg
    12001 cccccaagga gtgggtgcgc ctctcttcat gacttccgcc tccctgcccg cggcctccct
    12061 gaacctgctg ggcgtggatt atctgcacgt gcgcaccggc gaccagggcg atctctatct
    12121 cacccggcat ggcgcgccct tccgcgagca cctgttgccg gagaactggt acgacatggc
    12181 ctggttcgag cggaaccgcg agaagctgat cgggaccggg acggtctacc gggtccgcac
    12241 ccgcccgatc cgcggctgct cgaaggacct ggtcgtcaag tggtgccgcg tcggcgagga
    12301 ggtgccgatg gacacgttca cgctgagcaa gttcgtcgag gcggaattca acagcccgta
    12361 cgaggaattt tccctggtga tggagatgcg ggcccgttcc acgcaaggca tggtccggac
    12421 gcataagccg ctgggcattt acgtgccggc gaaaaagctg aagctatggc agaccggccg
    12481 ctcgctttcc cgaatggacc agaagaaggc caagtaccgc gacgtcgagc tggacatcta
    12541 tcgccagtac atcctgattt acgagtggat caagggcgtc aacgccacgg aggcctttga
    12601 acggatcctg ccggatgccc ggcagcggag gcaggaactc atcgccttca ccgaccgcgt
    12661 gacgaacgac ctgcaccgga aaggcttccg ggtgctggac accaagccgg agcacttcat
    12721 cgtccgtccc cgtccggacg gcagtctcct gcgcacgccg ggaggcgaaa tcgcgtacgc
    12781 gctggtggac ttcgaactgc tcgagcgcac gcccgagcgg gagcaggaag tcaccgcctc
    12841 gcggcgggcc gcctatctcc agcaccagaa gaaccgcttc aacgactcgg ccggcaagac
    12901 attcccgggg catctcacgc cctgccgcgt gatgggagtc gactacgtgt acgggcattc
    12961 cgagagcacg cagggccggc tttggatcgt cggttgcgac ccctccctgt ttgactactt
    13021 cctgcccgag cgatggcgcc gcacgccgcg gcgggcgctg tcgaaaacgg cgcagacgtt
    13081 ctatacccgc agcaaggatc tgatcaatct ggtctggaag gtgtcccgtg tcggggaacg
    13141 gccggatccg gaggcctcct cggaagcccg cgtacggatc acggagcacg gatacaacag
    13201 ccccttcgag gaattcgcgc tggcgctgga gttagggcgg cggggtctcg ccaccaccta
    13261 tccgcgagcc atctacatga cggggcaaga ggcggcgccc gacgcctaca cgctggaccc
    13321 cagccgctac gtgtcgcacc gcgggattct gaccccggcg ggcgatcccg cccttcggac
    13381 ggaccacaat tacattaccg tctggggctt ttggaacggg tccgacgagt tgctggccac
    13441 gcgcgatgtc gagtattgcc ggggcgtgga cctgctccgg gccttggagg acggcctgat
    13501 cgacgcggac gagttcacgg cgctccttga ccgggcacgc gcggaattgg ctcatgccgg
    13561 gttcgaggat ctgaacctca agggcacgca cgtcatgcta tccatgaatc cgcagggcca
    13621 cctgattcgc caggagagcg gtagcccggc cctgcgcttt tccaacttca gcttcctgcg
    13681 aaccaaatag aaccggataa ctcgatggtc agacacccct gcgccacact ttgcaaggtg
    13741 tggcgcaggg gggatgagta atatccttta tttacaggtg tatttcgcgc tttttccgat
    13801 gagtactatg cggccacact ttgtaaagta tggcgagggc cggcttcagc cgccatgccc
    13861 ctgcgtcaca ttcccttcca acgaaacgat cacctcatcc atggacagat ccccgcgccc
    13921 cgtgttccgc acggcctcct gcaccaggtt cgtcaacttc ccgcaacacg gcacctccat
    13981 ccgcacgacc agcacgcggg ggatgctcgg ctcggacaag atcgacgcga gcttttccac
    14041 gtagccctcg gtccggtcaa gtttaggaca tcccaccacc accgatcgcc cgcgcaggaa
    14101 ccgccagtgc atgtcggccg aggccagcgg cgcgcaggtg ctcatgatca ccagttccct
    14161 gcccttgaaa aagggcgccc cgggcggcac caggtgcatc tggaccggcc attggcgcaa
    14221 ctcggaggca ttcacctgcg ggggcagccc ggcatgtccg gcgacggcgg acgccggccg
    14281 gcgcccctcc ggaaaatccc ggaccgccat ccccggacaa cctcccggcc tcgccgtatg
    14341 ctcgcgcggc tcatgccggg aggcggcggc atcaaaagcc cggaccgccg ccgcgccgcc
    14401 cgactgcgcg acgtgccgac gagtagccgc ctcgtcgtac tcggccgctt ccgcctcaac
    14461 gattttgagc gcgccggtcg gacactcccc cacgcagtcg ccgaatccat cgcaatactg
    14521 ctcccggacc aggcgcgcct tcccatccac gatccgcagc gccccctccg cgcaggccgt
    14581 cacgcacgcg ccgcacccgt tgcagagggc ttcgtcgatg gtgatgatct tccgcttcat
    14641 gtcaggccgc gctgcccagc gccgtcgcca ggtccttctc caccgcgccc acgggctgaa
    14701 tgccgaactt ctccaccagc accttgatca cgggcggcgt caggaaggca ggcaggctgg
    14761 gtccaagccg gatgttccgg atgcccaggt gcagcagggt cagcagaatg caaacggcct
    14821 tctgctcata ccaggacagg atcagcgaca gcgggagatc gttgacgcca cacccgaacg
    14881 cgcccgccag cgcggaggcg atcttgatgg ccgagtaggc atcgttgcat tgccccacat
    14941 ccagcaagcg gggcaggccg gcgaccgtgc cgaaatccag cttgttgaag cggtacttac
    15001 cgcacgcgag cgtcaggatc acgcagtcct tgggaaccgc ttgggcaaac tccgtgtagt
    15061 aatcacgacc cggcttggcc ccgtcgcagc cgccgatgag gaagaagtgc cggatgtcgc
    15121 ccttcttcac cgccccgatc acctgatccg ccacgcccag caccgcgtgg tggccgaagc
    15181 ctaccaggat ggtcttttcc ggctcatccg ccgcgaaccc gggcgccgac cgcgcggcct
    15241 cgatcagcgg cccgaagtcg cgccgcgcca ggtgccggac gcccggccag gccaccagtc
    15301 cgctggtgaa aaggcggccg gcatagctct ctttcggttt ctggatgcag ttggtggtca
    15361 tcaatatcgc gccggggaag gcatcgaatt ccttccgctg atcctgccac gccccgccgt
    15421 agttgccgac gaggtggcgg tacttcttca ggccgggata gccgtgggcc ggcagcatct
    15481 cgccgtgggt gtacacgtgg actccggtcc cttccgtctg cttcagcaat tcctccaggt
    15541 ccttgagatc gtgaccggaa accaggatgg ccttgccctt gacgggcgtg atccgcaccg
    15601 gtgtcgggac gggatgccca taggcgcccg tgttcgcggc gtccagcagc cccatgacgg
    15661 tcagattgac ttcgccgcag cgcaagttca tgcccaggag ttgatccacc gtgggcgcct
    15721 cctgcgtcag gaaatccagc gcctcatgca tgaaggcgta taccgactcg tcctccttgc
    15781 cgaggatcag cgcatggtcg gcgtacgccg ccatgccctt cagcccgtac atcagcagct
    15841 cctgcaagcc ggtgatgtcc gcgccgcgaa ccgccatgcg gccctcgacg gagacctgct
    15901 cgccctgccg gatcagcccg tcgaggtcgg ccgccggctg ccaggcggcc ggtccgctca
    15961 acttctccgc cgccacgccc ccggcggcgc acgccttctc gtacagggcc cgggcccggt
    16021 ccttgaggag cgcggcccgg cggagcatga cttcgatgcg gcgcggatcg aaattgacgt
    16081 tggtgaccgt cgtgaagagc gactcgagcg cgaacacgtc cacctcgtga tcgcgcgcgc
    16141 cgagttgccg ggcgcgatgg gcatactggc tgatgccctt ctgcgcgtgc accagcaggt
    16201 ccagcagcgc ggcggtgtcc ggatccttgc cgcatacgcc gaacgacgtg cagccggttc
    16261 ccttggccgt ttgctcgcat tgatagcaga acatgatgtt tctcctgtgt tgaatgtacg
    16321 ccccattcta ccgcattccg cccggcgtcg tcttgaccca agtcaatagt ttcgcttttc
    16381 ccggcaggct cggtaatcct gccggagagt tcgggcaaac cacccttgat caatgccttc
    16441 ggaccaggcg acgccggtcg ccatttacct tgcggggcgc ggcagatacg atattttatc
    16501 ccctatgaac tcgcgcgcgc ttctccttgt cctggccctg ggcgcggccg gccccggctg
    16561 cgccctggtc cgcagcggcg tgggccgggc catggcgccc atggcggagg atctggccgc
    16621 ctcgctccag cagcaatcgg atgtcgaact cgtgcgcgag ggcgcgcccg cctacctgct
    16681 gttgctggac ggcctcgccg actccggctc ccggcagccg cgcatcctgc tggcggcggc
    16741 cgaggccaac acctcctacg ccatggcgtt cctggaccag gccgaccgcg agcgcgccaa
    16801 ggccttccac gctcgggcgc gcgactacgg gctagacgtg ctgcgccgca accgcaagtt
    16861 ccgcgccgcc gaacgcgcgc ccctgatgga cttcgtgtac cctgtcgtcc ctgggcagtt
    16921 cacaaccc
//
LOCUS       DAOOTK010000062        16832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2351_length_16832_cov_6.543661, whole genome shotgun sequence.
ACCESSION   DAOOTK010000062 DAOOTK010000000
VERSION     DAOOTK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16832
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2351_length_16832_cov_6.543661"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1863
                     /locus_tag="PK634_08285"
     CDS             61..1863
                     /locus_tag="PK634_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW97770.1"
                     /translation="MKFTAFVVTISVCIIFPLNRASAQGSLTPSGAPAPTMKTLDQIE
                     PRIPINSLPYFITNSGSYYLAASLTVGDDHGVVIQADNVTLDLMGFTLQGGTGYGIYA
                     PNAQRNAIIRNGIVSGWGGSGVDLANASGCLVEGITSVSNNNMGISIGPESRARNCAA
                     DLNSSGITAAGPHSTVRDCFARRNMYNGIDAGTFVAVEDCEATSNALHGITCSYDCQV
                     RRNRCVGNGHTGGNGAGIQTAGARNRIENNEAIGNYRGLDIYESPHWILDNVVEKNVD
                     NYSISYDPNFTNDIRLLVSELPLTVGRDCFVQITCDITTSTGGNGITVEGERVTIDLG
                     GHMLYGPGAGGCGIYQDASGKGLTVRNGRLDSWSSTDQAAIQAGQYALIEDVRIDNAF
                     NGIRAEYNATVRNIQLESCYGRCIWTGEKSLVEDCEIKSGVGEGIFVGNDSFVKSCRI
                     RTAYFEDHGNALVAGERALVTDCIVDNVSSGIVVGAHSTVENCAVSQSIGTGITAAEW
                     CVIRNNRVFLNGSYGASGILLTGGGTLVAGNYVAENGGYGVDGGTNLIALYQNVASAN
                     HHGHWNVVAANGVSQFTTPASASTNPVPNMEYSP"
     gene            1874..2749
                     /locus_tag="PK634_08290"
     CDS             1874..2749
                     /locus_tag="PK634_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97771.1"
                     /translation="MNPRFILCGAILALCGLTACGASTIDSLHPYAYGANIGWINALG
                     DTAHGAVIGQYYCTGYVWSANGGWIGLGNGPANGWRYGNADAADWGVNHDGEGRLTGQ
                     AYGANVGWLTFEQTHGQPRVDLRTGNFSGYAWGANVGWISLSNVQAFVRTTRLDPGPD
                     TDGDGVPDPWEYRHTNDLTVLDGGSHDADGDGATDSNEYGADTDPFYDGSLFEIVGFA
                     ARNPSNTVLWTSRPTRLYRVEATNTLAKGSTGWPDVGGGLIGPPPSSPTEMTVGDVTQ
                     PSRDYRVRAILPLSE"
     gene            2833..3738
                     /locus_tag="PK634_08295"
     CDS             2833..3738
                     /locus_tag="PK634_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97772.1"
                     /translation="MMERFLRDIFWVTGPLTGAELLDRARADVLPLWRECRRIPALRR
                     ILFGRRYLRLDRAVEGYARLSPSIRREFLTYTVLGLKEQSTLAAKKAEDLRRAAAAIS
                     QDKINLAREIAVSAMAELNDRALEERFCFLIAGDVIYNMAHRVPRPEKSTGEMVRGSD
                     LDIVVVAADDLPADAIQALDALIYKKKHYLLVHPDYREEVDYLIKDFRRVREQTDFDT
                     FESMVACKIMDESRLLYGSRALFQQVKGLLHERQVPQRLLHLEAAAVKEREEAEQALL
                     APEERFSAELYNHLFFTQEEGEEIY"
     gene            complement(4054..5082)
                     /locus_tag="PK634_08300"
     CDS             complement(4054..5082)
                     /locus_tag="PK634_08300"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOW97773.1"
                     /translation="MPCKVIITRDFDHVSEVAARYVATDIRNVLEKRPEYNIGLATGM
                     SPSGLYKDLAKTANNGGCDSSRIRSFNLDEYIGLPGENPQQRALHPESYSFFMIQELF
                     GLLQKKFRETNVPWGCLIDQEKFIAELKANPGDWEEHGKDKGRSIVIKAAAKSEYLRW
                     VRRGIQEAYEAKIKRMGGIDLHIVGVGGRGHVGFHEAGIPFAGSRVLIVKLDDNTIQN
                     AVTDGHFRSIEESPRYAVSMGAELIYEARKVLLVAMGKRKAEPIVASLAEDPTDAVPI
                     SYGQIFSQRGGEMIYVIDREAAAGVYERMDAIKARGVQIEDVSSGSASRRVQDLIFFR
                     NPETGLMG"
     gene            complement(5103..6458)
                     /gene="glmM"
                     /locus_tag="PK634_08305"
     CDS             complement(5103..6458)
                     /gene="glmM"
                     /locus_tag="PK634_08305"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOW97774.1"
                     /translation="MARLFGTDGVRGVANVYPMTAEMALELGRAAAYVCKRHKKIGPH
                     RIVIGKDTRVSGYMLETALTAGITSMGVDVLLVGPMPTPGIAFITQSMRADAGMVISA
                     SHNPFQDNGIKIFSRDGFKLPDAEEDEIERLITSGEIKDIRSTAHDIGKAHRIDDAVG
                     RYIVFCKNTVPEDVSLDGLKVVLDCANGATYRVAPIIFWELGAEVTAIHCEPDGTNIN
                     ANCGSQHTQDLSAKVRELKADVGLAFDGDGDRLIAVDENGQEITGDHILTICAKMLKE
                     SGRLTNNLVISTVMSNFGFGLALKELGIQHGAAKVGDRYVLEMMQQKGAVLGGEASGH
                     IILLEHHTTGDGILSALQLLVAMRHQNRKLSELATLMQMFPQKTINVDVAKKPPLEGL
                     ADLQAAIKAAEAELGEKGRVLIRYSGTQSMCRVMVEGPNDEIVNRLAKGLADQVKKSI
                     G"
     gene            complement(6479..7696)
                     /locus_tag="PK634_08310"
     CDS             complement(6479..7696)
                     /locus_tag="PK634_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOW97775.1"
                     /translation="MESSPAVKALLQRGVVMPCPAAVEVGDDVHPERIAPGVVLHAGS
                     AVRGAETAIGAGSVIGGEAPATVEDCQLGKKVALKGGYFSGATFLDGANMGSAAHVRP
                     GTLLEEEAGGAHAVGLKQTIFLPYVTAGSLINFCDALMAGGTSRKNHSEIGSSYIHFN
                     FTPHQDKATPSLIGDVPRGVMLDRPAIFLGGQGGLVGPVRIAFGTVIPAGQICRRDIL
                     EENRLYAGAPAAGDGGLRPYSAQLYRSVARVVRNNLVYIGNIWALRRWYEQVRKPWMT
                     GDPIAQACYEGAVARLDEVLEERVKRLGDLAGRMAKSLELAKKESLPEPVLARQRELM
                     ERWPEMEAVLKRGPGEGTGAADRDGFLQAWSAADRGAGYLKAIAALPENARRAGSAWL
                     QALVDELSALWKD"
     gene            complement(7755..8498)
                     /locus_tag="PK634_08315"
     CDS             complement(7755..8498)
                     /locus_tag="PK634_08315"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOW97776.1"
                     /translation="MKGADAIRETVERVGRARRIAALTGAGISTPSGIPDFRSADGLY
                     ADERNANVFDIGEFHRRPEHFYRFAREFYPMLQRAQPNAAHRALAAWEREGKEVHIAT
                     QNIDDLHQRAGSTRVYPVHGTVETSACQDCGVARRTSELVPEVMTGRVPRCGCGGVFK
                     PDITFFGEMLPEEAWNRSVEAMHLADLVLVIGTSLAVYPAASLPDQRRPAAALIVVNR
                     DPTPLDGAADVVLRGDLAEVFGRLEVSPP"
     gene            complement(8510..8686)
                     /locus_tag="PK634_08320"
     CDS             complement(8510..8686)
                     /locus_tag="PK634_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97777.1"
                     /translation="MSKQKILAMVLLILAAIVFIINRERTSVDLLVTTVKSIQSFVFL
                     GFTALGVVIGVLLK"
     gene            complement(8753..9544)
                     /gene="gloB"
                     /locus_tag="PK634_08325"
     CDS             complement(8753..9544)
                     /gene="gloB"
                     /locus_tag="PK634_08325"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOW97778.1"
                     /translation="MKTGRCFEHPLRDALVVIVPVLGDNFSYLLVAGDRAAAVDPADA
                     APLLEQLDARGLKLEWILNTHHHFDHVAGNEELKARTGCRVAGPGCAHIPGLDQAVAD
                     GDRLSVGRQTIEVIGTPGHTRSHLCYYLPSGAALWTGDTLFTGGCGRLIEGGSATMWG
                     SLHTLAMLPDETLVFCGHDYAEENLQFAALLQPDHPAIHQRLVRIQALIREGKATVPS
                     TLAEEKATNPFLRADTPELMQRLGLAGKSAADVFAELRHRKDLFA"
     gene            complement(9522..10412)
                     /locus_tag="PK634_08330"
     CDS             complement(9522..10412)
                     /locus_tag="PK634_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOW97779.1"
                     /translation="MSEAPGSIRVGVAGWSYPDWEGFVYSRREKDKLRFVAGYLDLIE
                     INSTFYRPPAAKTAGAWVRQTADLPEFFFSAKLHRDFTHEGRFSEAAARAFLDGLKPI
                     LDAGRLRHILAQFPFTFAESPEARSLLRAITESLRGTAALVLELRHRSWQASAALEFL
                     VGLGVTVANLDYPVGKDSFDLRECRIGEHGYLRLHGRNRVAWFDRKAGRNETYNYLYS
                     KPEVDDIRARAVSLAKTFRSLTIVANNHYQGKEVANALQLKAMLSGQKVRVPPDLLRQ
                     YPELAEVARERNPHEDGALF"
     gene            complement(10461..12380)
                     /locus_tag="PK634_08335"
     CDS             complement(10461..12380)
                     /locus_tag="PK634_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOW97780.1"
                     /translation="MGLFALATAADAARTGGVYAVTADTLDGGGRRAAGAAYVNDASL
                     GAIGAISGSDPVLKSGYAGQLMDLAGLNMSVAPIPMDEGTTGQVAAVARWDDDTVSPL
                     GANDIQWATPAYPFAAVAADGRLVASIVYADAQAAVTGLYFGLAGCATQTVMDIDSDN
                     FGIYAGDDIKDSWQVEFFGVDNPDGEADADPDGDRRDNRNEYVTGTGPDDEFSFFNLW
                     NERIEHVATQGDVFYHPRLDSRTYEVLRATALLTPDWSDSFAYSESLAGATCTVRDLN
                     IAADATYYRVRVEIIPTNCGEFALAYLSTLWDQHGYALALADFNEDGTLDMCVGYNGP
                     AQILLAATNEGFTPVVTDSGQRLGTGTVNGAAAADLNGDAHVDVLTAGPDGARIWYNS
                     GSAVFTGGLYAATNTYCTAVAIGDLNGDTNNDLFICRGSAAFGFKNLVLTNNRSGVFV
                     PVDQSAFILDRSYAVALGDLDNDGDLDAYVANSVANRCYRNDGRARFTLWGTDSRVDD
                     SRGVAIADLNGDGYRDVVVANAWPGPGIVYLNRTNGVLEAFPLGAASSDGTGVAVADL
                     DSDLRLDIAMSGWEDNTVWFGLDGTNFARSRQSLGYDRGQAVAVGDINRTTADDLVFV
                     HYDSTNTLWLNQCEP"
     gene            complement(12410..14896)
                     /locus_tag="PK634_08340"
     CDS             complement(12410..14896)
                     /locus_tag="PK634_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOW97781.1"
                     /translation="MKTRIYIFMAALLLGAAARAGAQVPPLLNYQGRVAVGGTNHDGT
                     GQFRFALVNGAGSVTFWSNGVNAVTTTVTKGLYAMLLGDTNQVNMAALPSSVFTNTDV
                     RLRVWFAPQGSALQQLSPDQRVAAAGYSLMAANADEVDGLHAVAFAQLSSSPMFTGTV
                     HAAAFAGNGASLTNINADQVDGLHAASFAQLSSSPTFTGTVHAAAFAGNGASLTNVNA
                     DQVDGVHAASFAQLSSSPTFTGTVHAAAFAGSGASLTGVNADQVDGLHAAAFAQLSSS
                     PTFTGTVHAAGFAGSGASLTGVNADQVDGLHAVSFAQLSSSPTFTGTVHAAAFAGSGA
                     SLTGVSADQLDGQHGWYYLNFANMTGAVTDALLSTNIPRLNASQNFSGANHFLSNMGI
                     GATNPQKRLEVRVQAAADGIRVDGAGSGVAPAISFSTNDTVHGELGQAAAGGQYSADA
                     AGGDFVIRNNNGKVLIQNGGGASALAVWTNNYVGIHKANPSTALDVNGTVTATAFDGD
                     GSALDHLYLNNATGTLLTVGQNWGDGPFQVYGSPWAMDQTNVNFGYYFHEWGMWQSFT
                     AGATGELVAVQAWVYSEAGGPWTATFNIYSGEGTGGALLYSHSVAGDGVVRARTFTLE
                     TPVLLARSNQYTFAVEGPSQRLTVRGSVDTYLHGHSNYATNYDYHFTTYFLTTNEPVP
                     ALVVDPYTHNVGIGTSTPTNKLHVVGTVQATAYITSSDSEMKENFQPVDAEAILDGVS
                     ALPVATWNFKDDTSGTHLGPMAQDFHAAFGLGNTERGIFTVDADGVALAAIQGLNRKL
                     ETALQTKEREIAELQQRVEQLEKRLPPPNP"
     gene            complement(14926..15372)
                     /locus_tag="PK634_08345"
     CDS             complement(14926..15372)
                     /locus_tag="PK634_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97782.1"
                     /translation="MKRAARWGIGLAVLVAAGRVGADTWTNAAGDTLEAEAVECDGRT
                     VLFRRPGGQEVRLPLFSLSGNEQRRVQERLNGPLLPADVRPAFDQAALQLERARLLLS
                     EQQLDNQEYAARHDQVIRTFVKACAALSHAENSDEVRQWVERLEAR"
     gene            complement(15369..16223)
                     /locus_tag="PK634_08350"
     CDS             complement(15369..16223)
                     /locus_tag="PK634_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97783.1"
                     /translation="MKRRTLALLLLAVALAGLIPESSAIPPLPSFRYYGLLTDEYGRP
                     VTFDQDTEIALDIDTNECARFTVNEQLGDGINYILEVPVDYAKSMRYASYAARTGDTV
                     AISVYRGGDFQPLMNTSSVPVVGAAGDAVRLNLGMGTDEDHDGMSDLWETQLIIWNSG
                     GQYSDVSEVLPGDDFDGDGVPNIDEFNAGTAPELAGDVFQVYKWMLTADNLLAVRFLT
                     VQGTTYEIQSAPAQVQGQGMQWTSTPFRVVSAGGDSNRYVATTSGFKYFYIEPREDMK
                     LIRLEVVK"
     gene            complement(16234..>16832)
                     /locus_tag="PK634_08355"
     CDS             complement(16234..>16832)
                     /locus_tag="PK634_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97784.1"
                     /translation="TVDFGHNFFGRVLAGGDHAFSSTEFATKIRSVGIWFSNYAANVS
                     YPVGLANTPEAYLIPVGVDVMRSPSDLTGNTLRSWQVLDQAIPVPYTIGSAEINEADW
                     IPLYDSLPDPLAEIRRYSALGAFDDTGVWGDSRINYSSRLIGRSVWNSQWYLIIPAGS
                     LNGDRALALEMFINGRTGDGEGVKDIKLLFETYSYSGN"
BASE COUNT         2807 a         5602 c         5460 g         2963 t
ORIGIN      
        1 tgcgggggaa cggggtacga ttgctgacaa tgctggccgg cgactaacaa gggggattcc
       61 gtgaagttca ctgccttcgt ggtgacgata tcggtgtgca tcatttttcc gttgaaccgg
      121 gcatccgccc aaggatcgct gacgccgtcg ggggcgccgg cgccgacgat gaagacactg
      181 gatcagatcg agccccgcat cccgatcaac agcctcccct acttcatcac caatagcggg
      241 tcgtattacc tggccgcatc cctcacggtc ggcgatgacc acggtgtcgt catccaggcc
      301 gacaacgtca cgctggacct gatgggcttc acactccagg gcggcacggg ctacggcatc
      361 tacgcgccca atgcccagcg aaacgcaatc atccgaaacg gcatcgtctc gggttggggc
      421 ggcagcgggg ttgatctagc caacgcatcg ggctgcctgg ttgagggcat cactagcgtg
      481 agcaacaata atatgggcat ttccatcggg cctgaaagcc gggcccggaa ctgcgcggcg
      541 gacctcaaca gcagcggcat tacggcggcc ggcccccact ccaccgtccg cgactgcttt
      601 gcgcgccgga acatgtacaa cggcattgac gcgggcacgt ttgtcgccgt ggaggattgt
      661 gaggctacat ccaatgccct ccacggaatc acctgcagct acgactgcca ggtccggcgc
      721 aaccgctgtg tcggcaacgg gcacaccggc gggaacggcg ccgggatcca gaccgctggc
      781 gcgcgcaacc ggatcgagaa caacgaagcc atcggcaact accgcggcct ggacatctac
      841 gaaagcccgc actggatcct ggacaacgtc gtggagaaga acgtggataa ctactccatc
      901 tcctatgacc ccaacttcac taacgatatc cggctgctgg ttagcgaatt gccgctcacc
      961 gttggccgtg actgcttcgt tcaaatcacc tgtgacatca ccacctcgac aggcggcaac
     1021 gggatcaccg tggagggcga acgggtgact attgacctgg gcgggcacat gctatacggg
     1081 cccggagcgg gcggctgcgg tatttatcag gatgccagcg gtaaggggct caccgttcga
     1141 aatggcaggt tggacagctg gtccagcacg gaccaggccg ccatccaagc cggtcaatat
     1201 gccctgatcg aggatgtccg gattgacaac gcgtttaacg gaatcagggc cgaatacaac
     1261 gcaacggtac ggaatatcca actggaaagc tgttatggac gctgtatctg gaccggggag
     1321 aagagtctcg tggaagattg cgagataaaa agcggcgtgg gcgaaggtat cttcgtcggc
     1381 aatgacagct ttgtgaaatc gtgtcggatc cgaacagctt acttcgagga tcacggaaac
     1441 gcccttgtcg ctggcgagcg cgccctggta acggactgca tcgtggataa tgtttcgtcg
     1501 ggtatcgtcg tcggggcaca cagcacggtg gagaattgcg ccgtgtcgca aagcatcgga
     1561 accggtatca ccgcggcgga gtggtgcgtc atccggaaca accgggtctt cttgaacgga
     1621 tcatacggcg ccagcggcat tctgctcacg ggcggcggga ccctggtcgc gggcaattac
     1681 gtggcggaga acggcggcta cggcgtggat ggcggaacca atctgatagc cttgtaccag
     1741 aacgtggcct cggcgaatca tcatggacac tggaatgtgg ttgctgcgaa tggcgtctcg
     1801 cagttcacca cacccgcttc ggcctccacg aacccggtgc ccaacatgga atacagcccc
     1861 tgacggacga accatgaatc cgcgtttcat cctgtgcggc gcgatcctgg ccctctgcgg
     1921 gctcacggcc tgcggcgctt ccacgattga ctctctccat ccttacgcct atggcgccaa
     1981 catcggctgg atcaacgcgc tgggcgacac ggcccacggc gcggtgatcg ggcagtacta
     2041 ctgcacgggc tacgtctgga gcgctaacgg cgggtggatc gggctgggca acggcccggc
     2101 gaacggctgg cggtacggca acgccgacgc cgccgactgg ggcgtgaacc acgacggcga
     2161 gggccggctg actggccagg cctacggcgc caacgtcggc tggctcacgt tcgagcagac
     2221 ccacggccaa ccgcgcgtgg acctgcgcac gggcaatttc tccggctacg cctggggcgc
     2281 gaacgtgggc tggatcagcc tgtcgaacgt gcaggcgttc gtgcggacca cccggctcga
     2341 cccggggccg gacaccgacg gggacggggt cccggacccg tgggaatacc ggcacaccaa
     2401 cgacctcacc gtgctggacg gcggaagcca cgacgccgac ggggacgggg caacggattc
     2461 aaacgaatac ggggcggaca ccgatccgtt ttacgacggc agtttgttcg agatcgtcgg
     2521 attcgcggcg cgtaacccgt cgaacacggt gctatggacc tcccggccga cgcgcctgta
     2581 ccgcgtggag gcgaccaaca cgctggcaaa gggttcgacc gggtggccgg atgtcggcgg
     2641 cggactgatc ggcccgcccc cctcctcgcc caccgagatg acggtcggcg acgtgacgca
     2701 accgtcgcgc gactacaggg tccgcgccat cctgccgctg tccgagtaga ttcagatcgc
     2761 acaggctgtc cagaaaacga tgcgccattc cgatcccatg acggaaagcc ggccaaccaa
     2821 agccagggct gcatgatgga acgctttctg cgggacattt tctgggtcac cggtccgctc
     2881 accggggcgg agttgctgga ccgcgcgcgg gccgacgtgc tgcccctctg gcgcgagtgc
     2941 cggcggatcc cggcgctgcg ccggatcctc ttcgggcggc gttatcttcg tcttgatcgc
     3001 gccgtcgagg gctacgcccg cttgtcccct tccatccggc gcgagttcct gacgtacacg
     3061 gtcctggggc tcaaggaaca gtccacgctg gcggcgaaga aagcggagga cctgcgccgc
     3121 gccgcggcgg cgatcagcca ggataaaatc aacctcgccc gggaaatcgc cgtctccgcc
     3181 atggccgagt tgaacgaccg cgcgctggag gagcggttct gcttcctgat cgccggcgac
     3241 gtgatctaca acatggccca ccgcgtgccg cgccccgaga agagcaccgg cgagatggtc
     3301 cgcgggtccg acctcgatat cgtggtcgtg gcggcggacg acctgcccgc ggacgcgatc
     3361 caggccctgg acgccctcat ctacaagaaa aagcattacc tgctcgtcca ccccgactac
     3421 cgcgaggagg tggactatct catcaaggat ttccgccgcg tccgggagca gacggacttc
     3481 gacacgttcg agtccatggt cgcctgcaag atcatggacg agagccgcct gctgtacggc
     3541 agccgcgcgc tgttccagca ggtcaagggc ctgctgcacg agcgccaggt cccccagcgc
     3601 ctcctccacc tggaagccgc ggccgtcaag gagcgcgagg aagccgaaca ggccctcctg
     3661 gcccccgagg agcgcttcag cgcggagctg tacaaccacc ttttcttcac ccaggaagag
     3721 ggcgaggaaa tttactagcc cggataatgc gcggcgcgca ttatccgccc aaagggccgt
     3781 gatgtgcccg cgttgcgggc acaaacgggg cttgccacgt tcgtattgtc gaatccttga
     3841 ttcgacaata cgtcacgtct tctggcacgc gaattgccta tgcaattcgc gtgcccagaa
     3901 gcgcttattg cgtgagcgtc tctacgctca tgcaataagc gggctagagc ggtttgcgaa
     3961 aaagcgtagc cgccagcgtg agcgggcggc acgggaaagc cgagtataac gtggccgcgc
     4021 cgttaccggc gcggccacgt ttcaactgat cctctacccc atcaacccgg tttccggatt
     4081 gcggaagaag atcaggtcct ggacgcggcg cgaggccgag ccgctgctca cgtcctcgat
     4141 ctgcaccccg cgcgccttga tcgcgtccat ccgttcgtac acgccggctg ccgcctcccg
     4201 gtcgatgacg tagatcatct cgccgccgcg ctgcgagaag atctgcccgt acgagatcgg
     4261 cacggcgtcg gtcggatcct cggccagcga ggcgacgatc ggctcggcct tccgtttgcc
     4321 catcgccacg aggagcacct tgcgggcctc gtagatcagc tccgcgccca tcgagaccgc
     4381 gtaccggggg ctttcctcga tgctccggaa atgcccgtcg gtgacggcgt tctggatcgt
     4441 gttgtcgtcc agcttcacga tcagcacccg gctgcccgcg aacgggatgc ccgcctcgtg
     4501 gaagcccacg tgcccgcggc cgccgacccc gacgatgtgc aggtcgatcc cgcccatccg
     4561 cttgatcttg gcctcgtagg cctcctggat cccgcggcgg acccagcgca ggtactcgga
     4621 cttcgcagcc gccttgatga cgatcgatcg acccttgtcc ttgccgtgct cctcccagtc
     4681 accgggattc gccttcagtt cggcgatgaa cttctcctgg tcgatcaggc agccccatgg
     4741 cacgttggtc tcccggaact tcttctgcaa caggccgaac agctcctgga tcatgaagaa
     4801 gctgtagctc tcggggtgca gggcgcgctg ctgcggattc tcgcccggca ggccgatgta
     4861 ctcgtccagg ttgaaactgc ggatgcgcga actgtcgcat cctccgttgt tggccgtctt
     4921 ggccaggtcc ttgtagagcc cggaaggcga catgcccgtg gccagtccga tgttgtattc
     4981 cggcctcttt tccagcacat tccgaatgtc cgtggccacg taccgggcgg ccacttcgct
     5041 gacatgatcg aaatcccgcg tgatgatgac cttgcagggc atgcgctttc tccttcttcg
     5101 aatcagccga tgctcttctt gacctggtcc gccaggccct tcgccaggcg gttcacgatt
     5161 tcgtcgttgg ggccttccac catcacgcgg cacatggact gggtgccgga atagcggatc
     5221 agcacgcggc ccttctcgcc gagttccgcc tccgcggcct tgatcgcggc ctgcaggtcg
     5281 gccagccctt ccagcggcgg tttcttcgcc acgtccacgt tgatggtctt ctgggggaac
     5341 atctgcatca gcgtcgccag ttcggagagc ttccggttct ggtgccgcat ggctacgaga
     5401 agttgcagcg cggacaggat gccgtcgccc gtggtgtggt gctcgaggag gatgatgtgg
     5461 ccggacgcct cgccgccgag cacggcgccc ttctgctgca tcatctccag cacgtaccgg
     5521 tcccccacct tggccgcccc gtgctggatg ccgagttcct tgagggccag gccgaatccg
     5581 aaattgctca tgaccgtcga gatcacgagg ttgttcgtca gccggccgct ctccttgagc
     5641 atcttggcgc agatcgtcag gatgtggtcg ccggtgatct cctggccgtt ctcgtccacg
     5701 gcgatcagcc ggtcgccgtc gccgtcgaac gccaggccga cgtcggcctt cagctcgcgg
     5761 accttcgccg aaagatcctg ggtgtgctgc gagccgcagt tcgcattgat attcgtgccg
     5821 tcgggctcgc aatggatggc cgtaacctcc gcgcccagtt cccagaagat gatcggcgcg
     5881 acgcggtacg tggccccgtt ggcgcagtcc agcacaacct tcagcccgtc caggctcaca
     5941 tcctcgggca cggtgttctt gcagaagaca atgtaccgcc ccacggcgtc gtcgatgcga
     6001 tgggccttgc cgatgtcgtg ggccgtggag cggatgtcct tgatctcgcc cgaggtgatc
     6061 agccgctcga tctcgtcctc ctcggcgtcg ggcagcttga agccgtcccg ggagaagatc
     6121 ttgatgccgt tgtcctggaa gggattgtgc gaggcggaga tcaccatccc ggcgtccgcg
     6181 cgcatgctct gcgtgatgaa cgcgatgccc ggcgtcggca tgggcccgac cagcaggacg
     6241 tccacgccca tcgaggtaat gccggcggtc agggcggtct ccagcatgta gccgctgacc
     6301 cgggtatcct tgccgatcac gatgcggtgc gggccgatct tcttgtgccg cttgcacacg
     6361 taggccgcgg cgcgtccgag ttccagcgcc atttccgccg tcatggggta gacattcgcc
     6421 accccgcgca cgccgtccgt cccgaatagc cttgccatgg ttgttctcct ggttgggtct
     6481 agtccttcca caaagccgag agttcgtcca ccagcgcctg cagccaggcc gagccggcgc
     6541 ggcgcgcgtt ctccggcagc gcggcaatcg ccttgagata gcccgcgccc cgatccgcgg
     6601 cgctccatgc ctgcaggaaa ccatcccgat ccgcggcgcc ggtcccttcc cccggccccc
     6661 gcttcaggac ggcctccatc tccggccagc gttccatcag ctcgcgctgg cgcgcgagca
     6721 cgggctcggg cagcgattcc ttcttcgcca gttccagcga tttcgccatc cgtcccgcca
     6781 ggtcgcccag gcgcttgacg cgctcctcca gcacctcgtc caggcgcgcc acggcgccct
     6841 cgtagcaggc ctgcgcgatc gggtcgccgg tcatccaggg cttccggacc tgctcgtacc
     6901 agcgccgcag cgcccagatg ttcccgatgt agaccaggtt gttcctcacc acgcgggcga
     6961 cgctgcgata cagctgcgcg ctgtacggcc gcaggccgcc gtcgccggcg gcgggcgcgc
     7021 ccgcgtagag ccggttttcc tcgagaatgt cccgccggca tatctggccc gccgggatca
     7081 ccgtgccgaa ggcgatccgc accgggccga ccagcccccc ctgcccgccc aggaagatcg
     7141 ccggccggtc gagcatgacg ccgcgcggca cgtcgccgat cagcgacggc gtggccttgt
     7201 cctggtgcgg cgtgaagttg aaatggatgt acgacgagcc gatctcgctg tggttcttcc
     7261 ggctggtccc gcccgccatc agcgcgtcgc agaaattgat caggctgccg gcggtgacgt
     7321 acgggaggaa gatcgtctgc ttgaggccga ccgcgtgggc gccgccggcc tcctcctcca
     7381 gcagcgtgcc cggccggacg tgcgccgcgc tgcccatgtt cgcgccgtcg aggaacgtcg
     7441 cgccggagaa atagccgcct ttgagcgcca ccttctttcc cagctggcaa tcctccaccg
     7501 tggccggcgc ctcgccgccg atcaccgagc cggcgccgat cgccgtctcg gcgccacgca
     7561 ccgcgctgcc cgcatgaagc acaacacccg gagcaatccg ctccgggtgg acatcgtcgc
     7621 cgacctcgac cgcggccgga cagggcatca cgacgccgcg ttgcaagagg gcttttaccg
     7681 ctgggctgga ttccataggc ttctccaaaa gtgggcgcat tctagaggcg gcaggaccgg
     7741 catctcaagc agaatcacgg cggcgaaacc tccagccgcc cgaacacctc cgccaggtcg
     7801 ccgcgcagca cgacgtccgc cgcgccgtcc aggggcgtcg ggtcccggtt aaccacgatc
     7861 agggcggcgg ccggccggcg ctggtccggc agcgaggcgg ccgggtatac ggccaagctc
     7921 gtgccgatca ccaggaccag gtccgcgaga tgcatggcct ccaccgaccg gttccaggcc
     7981 tcttccggga gcatctcgcc gaagaacgtg atgtccggct tgaacacccc gccgcaaccg
     8041 cagcggggga cgcgtccggt catgacctcg gggaccagtt ccgacgtgcg ccgtgcgacg
     8101 ccgcagtcct ggcaggcgct ggtctcgacc gtgccgtgga cgggatacac gcgggtcgat
     8161 cccgcgcgct gatgcaggtc gtcaatgttc tgcgtggcga tgtggacttc cttgccttcg
     8221 cgctcccagg cggccagggc ccggtgggcc gcgttgggtt gcgcccgctg gagcatggga
     8281 tagaattcgc gggcgaagcg gtagaaatgc tccgggcggc ggtggaactc cccgatgtcg
     8341 aacacgttgg cgtttcgctc gtcggcgtag agcccgtccg cggaccggaa atcggggatg
     8401 ccggacggcg tcgagatgcc ggcgccggtc agcgccgcga tccggcgcgc ccggccgacc
     8461 cgctcaacgg tttcgcggat ggcgtccgcc cctttcatcg ggaccgcggt tacttcagca
     8521 gcacgccgat cacgacgccg agcgccgtga agccgaggaa gacgaaggac tggatgcttt
     8581 tcaccgtggt caccaggagg tctacgctgg tccgctcgcg gttgatgatg aacacgatcg
     8641 ccgccaggat caacagcacc atcgccagta ttttctgttt gctcatgagg cttctcccga
     8701 aatcggttgg attatgcccg gcccggccgc ggttagcagc gaaaaaatcc gcttacgcaa
     8761 acaggtcctt cctgtgccgc agttccgcga acacgtccgc cgccgatttc ccggccagtc
     8821 ccagccgttg cattagttcc ggcgtatcgg cgcgcagaaa cgggttcgtg gctttttctt
     8881 cggccagcgt ggacggcacc gtggccttcc cttcccggat cagcgcctgg atgcgcacca
     8941 gccgctgatg aatcgccgga tggtccggct gcagcagcgc ggcaaattga aggttctcct
     9001 cggcatagtc gtgcccgcag aagaccagcg tctcgtcggg cagcatggcg agcgtatgca
     9061 gcgaacccca catcgtcgcc gagccgccct cgatcagccg tccgcagccg ccggtgaaga
     9121 gcgtatcgcc cgtccacagc gcggcgcccg acggaaggta gtagcagaga tgacttcgcg
     9181 tatggccggg cgtgccgatc acctcgatgg tctgccgccc caccgagagg cggtcgccat
     9241 ccgccacggc ctggtcgagc ccggggatat gcgcgcagcc ggggcccgcg acccggcagc
     9301 ccgttcgggc tttcagttcc tcgttgccgg cgacatggtc gaagtggtga tgcgtgttga
     9361 ggatccattc cagcttcagg ccccgggcat ccagctgttc gagcagcggg gcggcgtccg
     9421 ccgggtccac ggccgcggcc cggtcgcccg cgacaaggag atagctgaaa ttgtcgccca
     9481 gcacgggcac gatcacgacg agcgcgtcgc gcagcggatg ctcaaaacag cgccccgtct
     9541 tcatggggat tcctttcgcg ggcgacttcc gccagctctg ggtattgtcg cagaagatcc
     9601 ggcggcacgc gaactttctg gccggagagc atcgccttga gctgcaatgc gttggcgact
     9661 tccttgccct ggtagtggtt gttggcgacg atcgtgaggg agcggaacgt ctttgccagg
     9721 gaaacggccc gggcgcggat gtcgtccact tccggcttcg agtacaggta attgtaggtt
     9781 tcgttccttc ccgctttccg gtcgaaccac gccacccggt tccgcccgtg caggcgcaga
     9841 tacccatgct cgccgattcg gcattcgcgc aggtcgaagg agtcctttcc aacggggtag
     9901 tcgaggttgg ccacggttac gcctagtccc accaggaact cgagggccgc ggaagcctgc
     9961 caggaccggt ggcgcagttc cagcaccagg gccgccgtgc cgcgcagcga ttcggtgatg
    10021 gctcgcagca gtgagcgcgc ctccggggat tccgcgaaag tgaacgggaa ctgggcgagg
    10081 atgtgccgca gccggcccgc gtccaggatg ggcttcaatc cgtcgagaaa ggcgcgggcc
    10141 gcggcttccg agaaacgccc ttcatgagtg aagtcgcggt gaagcttggc gctgaagaag
    10201 aactccggga ggtccgctgt ctgccggacc caggcgccgg ccgtctttgc ggcgggcggg
    10261 cggtagaagg tgctgttgat ctcgatcagg tcgagatagc ccgcgacgaa ccgcagcttg
    10321 tccttttccc ggcgggagta aacgaatccc tcccaatccg ggtaggacca gccggccacc
    10381 cccacgcgaa tactgcccgg ggcttcgctc atgcgttccg agtataggcc gttcgcccgg
    10441 cggatacccg gggaaaatgg ctacggctcg cactggttga gccagagcgt gttcgtggaa
    10501 tcgtagtgta cgaagacgag gtcgtcggcg gtcgtgcgat tgatgtcccc caccgcgacg
    10561 gcctggcccc ggtcgtagcc cagcgactgg cggctccggg cgaagttggt gccgtcgagg
    10621 ccgaaccaga cggtgttgtc ctcccagccg gacatggcga tatccagccg aaggtcgctg
    10681 tccaggtccg ccacggccac gcccgtgccg tcggacgatg ccgcgcccag cgggaacgcc
    10741 tccagcacgc cgttggtccg gttgaggtac acgatgcccg ggccgggcca ggcgttcgcg
    10801 acgacgacgt cccggtaccc gtcgccgttc aagtcggcga tcgccacgcc gcggctgtcg
    10861 tccacgcggc tgtccgtccc ccacaaagtg aagcgggcgc ggccgtcgtt ccggtagcac
    10921 cggttcgcga cgctgttcgc gacgtaggcg tccaggtcgc cgtcgttgtc gagatcgccc
    10981 agcgccacgg cgtagctgcg gtcgagaatg aacgccgact ggtccacggg cacgaacacg
    11041 ccggagcggt tattggtcag cacaaggttc ttgaaaccga acgccgcgct gccccggcag
    11101 atgaacagat cattgttcgt gtcgccgttc aggtccccga tcgccacggc ggtgcaatag
    11161 gtattcgtcg ccgcatacag gccgccggtg aacacggcgc ttccgctgtt gtaccagatg
    11221 cgcgcgccgt ccgggcccgc cgtgagcacg tccacgtgcg cgtcgccgtt gaggtcggcc
    11281 gcggccgcgc cgttcaccgt gccggtgcca agccgctgac cgctgtcggt cacgacaggc
    11341 gtgaaaccct cgttggtggc cgcgagcagg atctgcgcag gcccgttgta acccacgcac
    11401 atgtccagcg ttccgtcctc attgaaatcc gccagcgcca gggcatagcc atgctggtcc
    11461 cacagcgtcg acaggtaggc cagggcgaac tcgccgcagt tggtggggat gatttcaacc
    11521 cggacgcggt agtaggtcgc gtcggcggcg atgttcagat cccgcacggt gcaggtggcg
    11581 ccggcgaggg actcggaata ggcgaacgaa tcgctccagt cgggggtcag cagggccgtg
    11641 gcccgcagga cctcgtaggt ccgggaatca agccgcggat ggtagaacac gtcgccctgc
    11701 gtcgctacgt gctcgatccg ttcgttccac aggttgaaga aggagaactc gtcgtcgggt
    11761 cccgtgccgg tgacgtattc gttccggttg tcccgccggt cgccgtccgg gtcggcgtcg
    11821 gcctcgccat ccgggttatc cacgccgaag aactcgacct gccaggaatc cttgatgtcg
    11881 tccccggcgt atatgccgaa gttgtccgaa tcgatatcca tgaccgtctg cgtcgcgcaa
    11941 cccgccaggc cgaagtagag ccccgtgacg gccgcttggg cgtcggcata cacgatggag
    12001 gccaccagcc ggccgtcggc cgcaacggcg gcgaacggat aggcgggcgt tgcccactgg
    12061 atatcgttgg cgcccagcgg cgaaacggta tcgtcatccc agcgcgccac ggcggccacc
    12121 tggccggtgg tgccttcgtc catcgggatg ggcgcgacgc tcatgttcag gccggccagg
    12181 tccatgagct gtcccgcgta gccgctcttc aggacgggat cgctgccgga aatcgccccg
    12241 atggcgccca ggctggcgtc gttgacatac gccgcgcccg ccgcgcgccg cccgccgccg
    12301 tccagcgtgt cggccgtgac cgcgtagacg ccgcccgtgc gcgccgcgtc cgccgcggtg
    12361 gcgagcgcga acagtcccac caagacggct atgccctgtc gtctcgtgat catggattgg
    12421 gcggaggcaa tcgcttctcc aactgttcca cgcgctgctg gagttccgcg atctcccgct
    12481 ccttcgtctg caaagcggtc tccagtttcc ggtttaagcc ctggatggcg gccagggcga
    12541 cgccgtcggc gtccacggtg aagatgcccc gctccgtgtt gccgagtccg aacgcggcgt
    12601 ggaaatcctg ggccatcggg cccaggtgcg tgccggacgt gtcatccttg aaattccacg
    12661 tcgccacggg cagcgccgag acgccgtcca ggatggcctc cgcgtccacg ggctggaaat
    12721 tctctttcat ctcgctgtcg ctcgatgtga tgtacgccgt ggcctggacc gtgccgacga
    12781 cgtgcagctt gttggtcgga gtcgaggtgc cgatgccgac gttgtgcgtg tacggatcga
    12841 cgaccagggc cgggaccggc tcgttggtgg tcaggaagta agtggtgaag tgataatcgt
    12901 aattggtcgc gtagttgctg tgcccgtgga gatacgtatc gacggagccc cgcaccgtaa
    12961 gcctttggga cggaccctcc acggcgaagg tatactgatt gctgcgggcc agcaaaaccg
    13021 gggtttccag tgtgaacgtg cgggcgcgca cgacgccatc gcctgccacg gaatgagagt
    13081 agagcaacgc cccccccgtt ccctcgccgc tgtaaatatt gaacgtggcc gtccaaggcc
    13141 cgcccgcttc cgagtacacc caggcctgaa cggcaaccaa ttcgccagtc gctccagcgg
    13201 tgaaggattg ccacatcccc cattcatgaa aatagtagcc gaaattgaca ttcgtctgat
    13261 ccatcgccca gggggagccg tagacctgaa acggcccgtc gccccaattc tggcccacgg
    13321 tcagcaacgt gccggtagcg ttgttcagat acaagtggtc gagcgcggag ccgtcgccat
    13381 caaatgcggt ggcggtcacc gttccgttca catcgagggc cgtggacggg ttggccttgt
    13441 ggatgcccac gtagttgttg gtccacacgg ccagggccga ggcgccgccg ccgttctgga
    13501 tgagcacctt gccgttgttg ttgcggatca cgaagtcgcc gcccgcggcg tcggcactgt
    13561 actggccgcc cgcggccgcc tggccgagtt ccccatgcac ggtgtcgttg gtgctgaagg
    13621 aaatcgccgg ggcgaccccg ctgccagcgc cgtccacccg gatgccgtcg gcggccgcct
    13681 gcacccggac ctccagccgc ttctgcgggt tcgtcgcgcc gatgcccata ttggatagga
    13741 aatggttggc cccgctgaag ttctgcgacg cgttgaggcg cgggatgttg gtggacagca
    13801 gcgcgtcggt caccgcgccc gtcatgttgg cgaagttcag gtagtaccag ccgtgctgtc
    13861 cgtccagttg gtccgcgctg acacccgtga gtgatgcgcc actgccggca aacgcggcgg
    13921 catggaccgt gcccgtgaac gttggactgg aactcaactg cgcgaaggac acggcgtgca
    13981 gcccgtccac ctggtccgcg ttgacccccg tcagggaggc gccgctcccc gcaaagccgg
    14041 ccgcatgaac cgtgcccgta aacgtcgggc tggagctcaa ctgggcaaag gccgccgcat
    14101 gcaatccgtc cacctggtca gcgttcacgc ccgtcagcga agcgccgctg cccgcgaacg
    14161 cggcggcatg aaccgtgccc gtgaacgtcg ggctggaact caactgggcg aaggacgcgg
    14221 catgtactcc gtccacctgg tcggcgttca cattcgtcaa tgaagcgccg ttccctgcga
    14281 aggcggccgc atgaacggtc cccgtgaacg tcgggctgga actcaactgg gcgaaggacg
    14341 ccgcatgcag tccatccacc tggtccgcgt tgatgttcgt cagtgaggcc ccgtttcctg
    14401 cgaaggcagc ggcatgaacc gtgcccgtga acatcgggct ggaactcaac tgggcaaagg
    14461 ccacggcgtg gaggccgtcc acttcgtcgg cgttggccgc cattagcgag tatccggccg
    14521 cggcgacgcg ttggtccggc gagagctgct gcaatgccga gccctggggc gcgaaccaca
    14581 cgcgcagccg cacgtcggtg ttggtgaaca cgctggacgg cagcgcggcc atgttgacct
    14641 ggttcgtgtc gcccagcagc atcgcgtaca ggcccttggt cacggtggtg gtcacggcgt
    14701 tgacaccgtt gctccagaat gtgacggacc cggccccgtt caccagcgcg aaccggaact
    14761 ggcccgtgcc gtcgtggttc gttccgccca cggcgacccg gccctggtag ttcagcagcg
    14821 ggggcacctg cgccccggcc cgggccgccg ccccgaggag cagcgcggcc atgaagatat
    14881 atatgcgcgt tttcaaggtt ggttctcctg tttgatgatc ctcgttcacc gcgcctcgag
    14941 ccgttccacc cactgccgga cctcgtcgct gttctcggca tgggacaacg cggcgcaggc
    15001 cttcacgaac gtgcggatga cctggtcgtg ccgcgcggcg tattcttgat tatccaactg
    15061 ctgctccgaa agaaggagtc gcgctcgctc caactggagc gcggcctggt caaaggctgg
    15121 gcgcacgtcg gccgggagca gcggcccgtt gagccgttcc tggacgcgcc gctgctcgtt
    15181 ccccgagaga ctgaaaagcg gcagccgaac ctcctgcccg cccgggcgcc ggaatagcac
    15241 ggtccggccg tcgcattcga ccgcctcggc ttcgagggtg tctccggccg cgttggtcca
    15301 ggtgtccgcg ccgacccggc ccgccgccac caggaccgcc aggccgatcc cccatcgcgc
    15361 cgcccgcttc atttcacgac ctccaggcgg atcagtttca tgtcctcccg cggctcaata
    15421 tagaagtatt taaagcccga agtcgtggcc acataacggt tgctgtcgcc gccggcgctg
    15481 acgacgcgga acggcgtgga ggtccactgc atcccctgcc cctggacctg cgcgggcgcg
    15541 gactggattt cgtaggtcgt gccctggacg gtcaggaacc gcacggcgag caggttgtcc
    15601 gccgtcagca tccacttgta gacctggaag acgtctcccg cgagttccgg tgcggtccca
    15661 gcgttgaact catcgatatt gggcacgccg tccccgtcga agtcgtcgcc gggcaacacc
    15721 tcgctgacgt ctgaatactg gccgcccgag ttccagatga tcaactgcgt ctcccacagg
    15781 tcggacatgc cgtcgtggtc ttcgtccgtc cccatgccca ggttgaggcg cacggcatcg
    15841 cccgcggccc cgaccaccgg cacggaggac gtgttcatca gcggctggaa atcgccgccg
    15901 cggtagacgc tgatggcgac cgtgtcgccg gtgcgcgcgg cgtacgacgc gtaccgcatg
    15961 ctcttcgcgt agtccaccgg cacttccagg atgtagttga tcccgtcgcc gagctgctcg
    16021 ttgacggtga accgcgcgca ttcgttggtg tcgatgtcga gcgcaatttc cgtgtcctgg
    16081 tcgaacgtaa ccgggcggcc gtactcgtcc gtgagcagcc cgtagtaccg aaaggacggc
    16141 agcggcggga tggcgctgga ctccgggatc aggcccgcaa gcgcgacggc cagcagcagc
    16201 aaagcaagcg tgcgtctttt catggtcgcc tctctaattt cctgaatacg aataggtttc
    16261 aaacagcagc ttgatgtcct tgaccccttc gccgtcgccg gtccggccgt tgatgaacat
    16321 ctcgagcgcc agcgcgcggt cgccgttcag cgagccggcg gggatgatga ggtaccactg
    16381 ggagttccag acggagcggc cgatcagccg gctgctgtag ttgatgcgcg agtcgcccca
    16441 gacgccggtg tcgtcgaacg cgccgagggc gctgtagcgt cggatctccg ccagcggatc
    16501 gggcagggag tcgtagagcg ggatccagtc ggcctcgttg atctccgcgc tgccgatcgt
    16561 gtacggcacc gggatggcct ggtccagcac ctgccacgag cgcagcgtgt tgccggtcag
    16621 gtccgacggc gagcgcatga cgtccacgcc gacggggatc aggtaggcct ccggcgtgtt
    16681 cgccagaccc accgggtacg acacgttcgc ggcatagttg ctgaaccaga tgccgacgct
    16741 gcggatctta gtcgcgaact cggtggagct gaacgcgtgg tcgccgccgg cgagcacccg
    16801 gccgaagaag ttgtggccga aatccacggt cg
//
LOCUS       DAOOTK010000063        16821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2356_length_16821_cov_5.690684, whole genome shotgun sequence.
ACCESSION   DAOOTK010000063 DAOOTK010000000
VERSION     DAOOTK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16821
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_16821_cov_5.690684"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK634_08360"
     CDS             complement(<1..370)
                     /locus_tag="PK634_08360"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOW97785.1"
                     /translation="MMKRRVWRWGIALALLAAGPMVRADLVPHPPSEAQAGDGMSTGD
                     RIAHYLEQKGWPKELVVLSISALPIVELRGAVPVGINLLGMNWAWVFFLSVVGNMIPI
                     PFILLFLGPFSRWCMKFRAGK"
     gene            complement(367..1059)
                     /locus_tag="PK634_08365"
     CDS             complement(367..1059)
                     /locus_tag="PK634_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW97786.1"
                     /translation="MSAEVKQSGSVAVVIPAFREERHIGGVVRAVRAEGFEAVVVDDG
                     SDDRTAEVAGEAGAVVIRHDRNQGKGVALQHGFEYAAAHGYEAAVTLDADGQHRPEEI
                     RRFVEAYGRGDAPVLIGNRMADTSTMPLIRRWTNRFMSWLLSRLMGQAVPDTQCGFRL
                     YRLDAIPPRDGASERFDAESEILLEIARRGVRIGSVPISTVYGEERSKVNPWTDTIRF
                     FRMLRRYRRERA"
     gene            complement(1056..2000)
                     /locus_tag="PK634_08370"
     CDS             complement(1056..2000)
                     /locus_tag="PK634_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOW97787.1"
                     /translation="MTGYWPYRIAAFLSRALPERLAYWVGLRFSDLFYRRDQKGRAAV
                     MGNIATVLRARGVQPARDALDGFARKTFQYFGKYLVDFFRFSRLTQEDIERRVSIEHR
                     ERLDQSLALGRGLILVTAHFGNWELGGAVIAGLGYPVNAVVLPQRLEKLNRLFQGQRE
                     RRGLRVIPMGSSVFNLIRCLRRKELLALLADRDFTGKSDPVEFFGKPAHMPVGPAWLS
                     FKTGAPVLVGFLIREVDDTFRLRLYPPILPGPDASIEGIRARINEILEKEIAERPYQW
                     FMFDPFWDAQAPVQAAQAPAPFDEAGPVDYVAGQGKTP"
     gene            complement(1997..3121)
                     /locus_tag="PK634_08375"
     CDS             complement(1997..3121)
                     /locus_tag="PK634_08375"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW97788.1"
                     /translation="MSRVLICYLVRNSGHHSAARNVQAALHKLDPSIETMCVDLLEYT
                     HPRWSAIVQKTYMVTIRRTPEVWEALYDRLWLEYLTRRIRRLVQRGNSRPLRDLMDRF
                     RPDVAVCTQAHPLGVLSAYAQRHRPDLPLWGIVTDYVPHRYWVVRWPPVRYVVPGEAA
                     GGRLALYGVHRPNLFPLGIPIHTGFVTERKTRVSREDGRRVLVMGGSRGLGARYRTIR
                     YLDRSTEDFTIDVVTGANRRLRAKLIRNRDKFRHPIRIRGYVKDVVALMHRASLLISK
                     PGGLTSAEAMAVGLPMVLVRPLPGQERGNTEVLVRHGAAVHLPEDRDVASVVTSLLAN
                     RELLDMMRERALDLGRPHAALDTARLILKDIAAPGGKAGA"
     gene            complement(3106..3987)
                     /gene="lipA"
                     /locus_tag="PK634_08380"
     CDS             complement(3106..3987)
                     /gene="lipA"
                     /locus_tag="PK634_08380"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOW97789.1"
                     /translation="MPRLPAWIRSDRRTDADFKRVQGLLSREGLNTVCVGARCPNRAE
                     CWNHGTATFLILGDTCTRGCAFCAVTPGRPAPPAADEPERVARAVNAMGLKHAVVTSV
                     TRDDLPDGGAGAFAATIRAIRSAAPGTSVEVLTPDFAGSESALAAVLEARPDVFAHNL
                     ETVRRLTPAIRASASYERSLAVLRFAASWTPPAAVKSGLMLGLGETDAEVMESLRDLR
                     GSGCRWLTLGQYLAPTARHAPVARFVTPAEFDGWAARARQMGFEPVASGPLVRSSYRA
                     AEGWAELAREDRACPAC"
     gene            complement(4034..4840)
                     /locus_tag="PK634_08385"
     CDS             complement(4034..4840)
                     /locus_tag="PK634_08385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOW97790.1"
                     /translation="MPWETKTAWAPPGRWNFPPAVRALLFGTVGLSLFQWVADDLTGG
                     AFTRWFGLSWLSLRRGLVWTPVTYIFLHGGVFHLLMNMLVLFFLGPETERALGSRKFL
                     LLYFISGLLGGLGWVWIEDSPLVTCIGASGAVFGILGAFAALFPNQPITLLLFYVVPV
                     TLRAWVLAVILGLVELMFLLSPGSGSVANAAHLAGGLAGYFYALGLVRGERSGARSWR
                     KVAKLFTPPPADDARELDRLLDKIANHGLHSLTPREREWLDRASRARRGS"
     gene            4925..5629
                     /gene="bioD"
                     /locus_tag="PK634_08390"
     CDS             4925..5629
                     /gene="bioD"
                     /locus_tag="PK634_08390"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOW97791.1"
                     /translation="MKGLFITGTDTGIGKTAVLAALLCALRRAGIDAAPMKPVQTGCR
                     PSRRGLVSPDLERALRAAGLRPDAAEKSLMCPYPFRPACSPHLAAAQAGVRIRLSAIR
                     KAFRRLSARHSYVLVEGAGGVLVPLNGNKTMLDLMRALALPVIVAARPGLGTLNHTLM
                     TLRELRRAGLKILGVILVCARRAPWGAIERSNLETIRRLGRVPAHRLRYRRGPGAPGD
                     AVRWIKEWNLRASRRA"
     gene            complement(5636..5932)
                     /locus_tag="PK634_08395"
     CDS             complement(5636..5932)
                     /locus_tag="PK634_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97792.1"
                     /translation="MTEQQFRDQAAAIVKDRPAKVWLVRSAGHRLDFLWGVGVSRTAE
                     PERLGRHGRHYLVGQDVPEELKPQLVALFDDFCQSDEVQQAESTVRPLRFVPAR"
     gene            complement(5992..7926)
                     /locus_tag="PK634_08400"
     CDS             complement(5992..7926)
                     /locus_tag="PK634_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW97793.1"
                     /translation="MQHNPDSKAGTPDPSRRSEDSGKRYHALFEAATDAIFLETLHGE
                     ILDCNGAAQQLFGYTREEMLRLRVTDLVPEDVARTLPSLITEELTTGGFFVEAVNRRR
                     SGELFPVEVSSRLVRIGEEAQIVVFVRDITERKKAASALEAEKERLAVTLRSIGDGVI
                     ATDETGLIVLINRVAETMTGWAQAEAVGRSLDEVFHIVNEKSRARADNPAQRVLKSGI
                     TAGLAGNTVLIARGGAERIIADSAAPIRDRDSKIIGVVVAFREITELRRMEEERLRAQ
                     KLESLGLLAGGIAHDFNNILTGILGNISLARMALQSGGDHPALLQEAEKACLRAKDLS
                     QQLLTFARGGAPVRQMTSMSSLVRDTVEFCLRGSNVRADMHVSADLWAAEVDPGQIAQ
                     VVNNVVINSKQAMPGGGVIVVRVENADLEQGGPHPVDPGRYVRITVKDAGSGIPREYL
                     QKVFDPFFTTKAQSSGLGLTASYAIVRKHGGNLLLESELGAGTVVHILLPASRAILPA
                     PPEEERPAPHGTGRILVMDDEDAVRNVAVRVLEYLGYEPTAARNGTQAVQEYRRAMET
                     GRRFAAVILDLTVPGEMGGREALEKLKALDPNVRAIVASGYSSDSCMSNYRACGFAGA
                     VIKPFTVNEVGRIIHAVLRE"
     gene            complement(8018..8986)
                     /gene="rfaD"
                     /locus_tag="PK634_08405"
     CDS             complement(8018..8986)
                     /gene="rfaD"
                     /locus_tag="PK634_08405"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOW97794.1"
                     /translation="MSTKFIVTGGAGFIGSNLVAALNARGEDHILVVDELGTDEKWKN
                     LVGLRYEDYMGRDDFRAAIQHDALTAVDAVFHLGACSSTTEKDAGFLADNNYRYTREL
                     CEWCLHNGARFIYASSAATYGDGESGYSDDDRHTLTLKPLNMYGYSKHMFDLWALRHA
                     LLDRIVGLKYFNVFGPREDHKGDMRSVVHKAYHQIRETGEVRLFKSYKPKYRDGEQVR
                     DFIFIQDAVDVTLFFLDHPGVSGLFNCGTGQARTWNDLAKAVFAAMGLEPRITYIDMP
                     PALREKYQYHTQADVLKLRKAGYDKPFTPLEEAAARYVKEWLSAPR"
     gene            complement(8983..9456)
                     /locus_tag="PK634_08410"
     CDS             complement(8983..9456)
                     /locus_tag="PK634_08410"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOW97795.1"
                     /translation="MNPRFSHEKLDVYRLELDFVRWSTVLLNELKSGDGPCTGEVRNQ
                     LDRAGLSMLLNTAEGNGRRRMPMRAKFFDDARGSAAECAACLDALAVKGAVPAERVED
                     GKVLLASIAAMLSKLVAYFSETRLIREDRAEYGAGAGGGNEGEEEREKEEGEAKP"
     gene            complement(9453..9995)
                     /gene="rfaE2"
                     /locus_tag="PK634_08415"
     CDS             complement(9453..9995)
                     /gene="rfaE2"
                     /locus_tag="PK634_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOW97796.1"
                     /translation="MRPAESKILSRETMAAERARLRAAGKKLVFTNGAFDLLHAGHVT
                     YLEFARQQGDALVVGLNSDASVRRYKGDKRPVNGQADRARVLAALECVDYVVVFDEDE
                     PADLIADLVPNVLVKGADWAHYVSGRDVVEKAGGRVVLAPMVEGRSTSGMIGKILQAH
                     SPSPSLPPSPTPTPSPPHDS"
     gene            complement(10027..11292)
                     /gene="icd"
                     /locus_tag="PK634_08420"
     CDS             complement(10027..11292)
                     /gene="icd"
                     /locus_tag="PK634_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOW97797.1"
                     /translation="MHYDRIQIPASGRKIALGPDRRLQVPDRPIIPFIEGDGTGPDIT
                     RAAMTAWNAAVEKAYAGRRRVEWMEVYAGEKANAVYGEPVWLPPETLEACREFKVSIK
                     GPLTTPVGGGIRSLNVALRQELDLYVCLRPVRWFEGVPSPVKHPERTDMVIFRENAED
                     IYAGIEWAAGTPEVKKVVDFLQREMGVKKIRFPGSSGIGIKPVSREGTARLVRAAIRY
                     AIEAGRPSVTLVHKGNIMKFTEGAFRDWGYRVAREEFGAEPLDGGPWHRLKNPRTGAD
                     LVVKDVIADAFLQQILTRPAEYSVIATLNLNGDYISDALAACVGGIGIAPGANINYDT
                     GAAVFEATHGTAPKYANQDKVNPGSLILSGEMMFRYLGWTEAADLIVRGMEQAIRAKT
                     VTYDFARLMDGAREVKCSEFGRLVAEAMS"
     gene            11434..11895
                     /locus_tag="PK634_08425"
     CDS             11434..11895
                     /locus_tag="PK634_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOW97798.1"
                     /translation="MTSSQDADRLMELLPKLMRGIHAYERNFFTCENITFPQLWALDF
                     VHEHPRCTMHQLATAMHSRQSTATGLVDRMQRLNLVRRERRRSDRRVVCVSLSEKGRS
                     MVRQITDQRRRMIRRLFSRMSERDRRQYLDILGRLVEQFSEPARARTGGHP"
     gene            11892..13238
                     /locus_tag="PK634_08430"
     CDS             11892..13238
                     /locus_tag="PK634_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW97799.1"
                     /translation="MRRLPILMAGLLALRPCFGQTNTEAPLTIERAFEMALERNEEAR
                     LADLAVKQRALLPWRSLSLLFPSLTADASRMIPEDEIVGESGSPVVADPTDRASLRLS
                     QPLFSSDAYAGYRSGRSLERAAIEDRVFRLRDLLFRVAATFIDCLKLEHVVGINRETL
                     TLAEEQRRTAEARYEAGEVPKTDVLRAEVEVSRARRELDVSRNQWQLAGAQLATLIGA
                     GDGPVSVQAPPEGLADRLEHADSLETLIAAALEQRDDLRRAAYEREAARWSLVKARRG
                     WWPTVSFDVEQTWADPGSLSQPDDFWTATFRASLPLFEGGARQLDAREARYALEEADL
                     VVSQLVKTVRLQVTEAWLRTRTARVLLDATQKELELARENYDVVSQRYRAGKATSLDQ
                     VDAFTQLSRARTSVANLTYDHQAAILHLLRQVGSLGERYVADTRSEPDRSARGRTE"
     gene            13235..14128
                     /locus_tag="PK634_08435"
     CDS             13235..14128
                     /locus_tag="PK634_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW97800.1"
                     /translation="MNLKSQWGGPWLAVLLASTAGAQPPPGGGREAAGEPIPVRVMRV
                     EPRDLQQALEYVGDIKARDEAVVYPTVTGKIAEKLKEDGARVRKDEALALVDRDEVGF
                     KFEKAPVKSPLGGIVGRVYVDLGANVTPQTPVALVVNMDQAKINLDIPERYLPRLAPG
                     QVALITVDAYDGIFTGRVSKISPVVDLATRTAPVEILAPNRAHELKPGMFARARLLME
                     ERKGILVVMKEAVLGMAPDQYVYVVEDDTARQRPVRLGLREAHEIEVLEGLTAGEAVV
                     IVGQQRLRDGLPVAVEEAAAP"
     gene            14147..>16821
                     /locus_tag="PK634_08440"
     CDS             14147..>16821
                     /locus_tag="PK634_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOW97801.1"
                     /translation="MNLPEFGVRRPVTNLMIFLGIIILSLFSLSRLGVDMMPEIEPPA
                     ISVVSLYPGASPEDVEVKVTEPLENQLASTPHLEKITSRSAEGVSAIQLKFTWGVNLD
                     EASNDIRDRIERAKRALPDIPDEMDNPFIFKFNTALMPFLILGVTADQNTPELNDLVD
                     RRVADQLRQLPGVGTVQLRGGLDRQINVWIDRGRLEGYGFSILDVKAALAAANVTQPV
                     GNIKSGLTDYLVRLPGEFASPDEINSVILGQDQGRLIYLKDVARVEDSHAEVSSVVRI
                     NGKSGLMMAVMKQTGVNTVEVADRVKKKLAEIEPSLPLNIRFHIMMDSSQDILDALSS
                     LRETVWIGGALVILVTWFFLRRVRPSLIIALTIPFSLLIAFIYLFLSGRTINIISLSS
                     LTIAIGMVVDNAVVVVDNVIRRLDQGERSAEASIFGTSEMYLAIAASTGTTVVVFLPL
                     LFVSGVVGIMFGELAVIITVTLLASLFTAATFSPMLCSKWLKPAARDGEAARGRLGSR
                     VYERFERWFTAWERAYARVLHWSVRRAWHRVLVLFGFTAAFAGSLLLAPRVGTEFIPE
                     EDSGDLRVSLQLPVGTRLEESDAVGRKVEAIFGAEVPEMRFMYMQSGQGGGMGAAFGG
                     ASGSHSISSGARLVPKEERTRSSREIAQAVRQRVRRLAGVLRTDIYAGNPMGRLITGT
                     GGKKVQIEVIGHSFEETDAVADRIRAALEQVEGAVDISVSREMKRPELRIAVDREKAA
                     ALGLDMQTVAETVTTAIEGSTATRYRERGETYDIFVRLEEAWRSKPEDLESLTVLSPY
                     TRRLVRLANIARVIETRGPLEIERQNRERVVRVECNTHERSLGDVVADARRAVAGMTL
                     PPDVLINWGGEAEEQAKAFGDLTLLLLLGIALVYMV"
BASE COUNT         2696 a         5755 c         5702 g         2668 t
ORIGIN      
        1 ccttgccggc ccggaacttc atgcaccacc gggagaacgg gccgaggaac agcaggatga
       61 acgggatggg gatcatgttg ccgacgacgg acaggaaaaa gacccacgcc cagttcatcc
      121 ccagcaggtt gatgcccacc ggcacggcgc cgcgcagctc gacgatcggc agggcggaga
      181 tcgacagcac gaccagttcc ttcggccagc ccttttgttc caggtagtgc gcgatgcggt
      241 ctcccgtgct catgccgtcc ccggcctgcg cctccgacgg cggatgcgga accaggtccg
      301 cccggaccat gggcccggcc gccaggaggg ccagcgcgat cccccacctc cacacgcgac
      361 gtttcatcat gctcgttccc tcctgtatcg cctcaacatc cggaagaagc ggatcgtatc
      421 ggtccaggga ttcaccttgc tgcgctcttc gccgtagacc gtcgagatcg gcacggagcc
      481 gatgcgaacc ccgcgccgcg cgatttccag caggatctcg gactcggcgt cgaaccgctc
      541 ggacgcgcca tccctcgggg ggatcgcgtc aagccggtag agccggaagc cgcactgggt
      601 gtccggcacg gcctgcccca tgaggcggct caacagccag ctcatgaagc ggttggtcca
      661 gcgacggatg agcggcatgg tggacgtgtc ggccatgcgg ttgccgatca gcacgggcgc
      721 gtcgccgcgg ccgtaggcct ccacgaaccg ccggatttcc tccggccggt gctgcccgtc
      781 ggcgtccagc gtcacggccg cttcgtagcc atgcgcggcc gcgtactcga acccgtgctg
      841 cagggcaacg cccttcccct ggtttcgatc gtgccggatg accacggcgc ccgcctcgcc
      901 ggccacctcc gccgtccggt cgtccgatcc gtcgtccacg accacggcct cgaaaccctc
      961 cgcccgaacg gcgcggacga ccccgccgat atgccgctcc tcccggaacg ccgggatcac
     1021 cacggccacc gacccggact gtttgacttc tgcgctcatg gggttttacc ctggccggca
     1081 acatagtcga ccggcccggc ttcgtcaaag ggggccgggg cctgcgcggc ctgaaccggg
     1141 gcctgggcat cccagaacgg gtcgaacatg aaccactggt acgggcgctc cgcgatttcc
     1201 ttttccagga tttcgttgat gcgggcccgt atcccttcga tggacgcgtc cgggcccggg
     1261 aggatcggcg gatagaggcg aagccggaac gtgtcgtcca cttcgcgaat gagaaagccc
     1321 accagcacgg gggcgccggt cttgaaggac agccaggcgg gcccgacggg catgtgggcg
     1381 ggcttgccga agaactcgac cgggtcgctc ttgccggtga aatcacggtc ggccagcagg
     1441 gccaggagct ccttgcgccg gaggcagcgg atgaggttga agacggaact gcccatcggg
     1501 atcacgcgga gcccccggcg ctcccgctgg ccctggaaca ggcggttcag cttttcgagc
     1561 cgctggggca gcacgaccgc gttcacgggg tagcccagcc ccgcgatcac ggcgccgccg
     1621 agttcccagt tgccgaaatg cgccgtcacg aggatcaggc cgcgccccag cgccagggac
     1681 tggtccaggc gctcgcggtg ttcgatgctg acgcgccgct cgatgtcctc ctgcgtcagc
     1741 cgggagaagc ggaagaaatc cacgagatac ttgccgaaat actggaacgt cttgcgggcg
     1801 aacccgtcca gcgcatcccg ggcgggctgc acgccgcgcg cccgcaggac ggtcgcgatg
     1861 ttccccatga ccgccgcgcg ccctttttga tcccgccggt agaacaggtc ggagaatcgc
     1921 aggccgaccc agtaggccag cctctcggga agggcgcggc tcaagaacgc ggcgatgcga
     1981 tagggccagt agccggtcat gcgcccgcct ttccgcccgg cgcggcgatg tccttgagga
     2041 tcagccgggc cgtgtccagc gcggcatgcg gccgccccag gtccaaggcc cgctcgcgca
     2101 tcatgtccag caactcccgg ttcgccagca gcgaggtcac gacgctggcg acgtcccggt
     2161 cctcgggcag gtggaccgcc gccccgtgcc ggaccagcac ctccgtgttg cccctctcct
     2221 gccccggcag cggccggacc aggaccatcg gcagcccgac cgccatggcc tcggcgctgg
     2281 tcaggccgcc tggcttgctg atcagcaggc tcgcgcgatg catgagggcg acgacgtcct
     2341 tgacgtaacc ccggatgcgg atcgggtgcc ggaacttgtc gcggttgcgg atcagcttgg
     2401 cgcgcagccg gcggttggcg ccggtgacga cgtcgatcgt gaagtcctcc gtgctgcggt
     2461 ccaggtaacg gatggtccgg tagcgcgcgc cgagcccgcg gctcccgccc atcaccagca
     2521 cgcggcgccc gtcctcgcgc gagacgcggg tcttgcgctc ggtgacgaag cccgtgtgga
     2581 tcgggatgcc gagcgggaac aggttcggac ggtgcacgcc gtagagggcc agccgcccgc
     2641 ccgcggcctc gccgggcacg acgtagcgca cggggggcca gcggacgacc cagtagcggt
     2701 gcggcacgta gtcggtcacg atcccccaca gcggcaggtc cggccggtgg cgctgggcgt
     2761 acgcgctcaa gacgccgagc ggatgcgcct gcgtgcagac ggcgacgtcg gggcggaaac
     2821 ggtccatgag atcgcgcagc ggccggctgt tgccgcgctg gacgagacgc cggattcggc
     2881 gcgtgaggta ctcgagccac aggcggtcgt agagcgcctc ccagacttcc ggcgtgcggc
     2941 ggatggtgac catgtaggtc ttctggacga tcgcgctcca ccgcgggtgc gtgtattcga
     3001 gcaggtccac gcacatggtc tcgatggacg gatccagctt gtggagcgcg gcctggacgt
     3061 tccgcgccgc ggagtggtgc ccgctgttcc gcaccaggta gcagatcagc acgcgggaca
     3121 tgcccggtcc tccctcgcca actccgccca gccctccgcc gcccggtacg aagaacgcac
     3181 gagcggcccc gacgccaccg gctcgaagcc catctgccgc gcgcgcgcag cccagccgtc
     3241 gaattcggcc ggcgtgacga accgcgccac gggggcatgc cgcgccgtcg gggccaggta
     3301 ttgccccagc gtgagccagc ggcaacccga cccgcgcagg tcgcgcaggc tctccatcac
     3361 ctcggcatcg gtctccccca ggcccagcat gagccccgat ttgaccgccg cgggaggggt
     3421 ccaggaagcc gcgaaccgca acacggccag cgagcgctcg tacgacgccg aggcgcggat
     3481 cgccggagtc aaccggcgga cggtttcgag attatgggca aacacgtccg gccgtgcctc
     3541 gagcaccgcg gccagcgcgg attccgaacc cgcgaaatcg ggcgtcagca cctcgacgct
     3601 ggtcccgggg gcggcggacc gtatcgcccg gatcgtcgcg gcaaaggccc cggccccgcc
     3661 gtcgggcagg tcgtcccggg tcacgctggt cacgaccgcg tgcttcaggc ccatggcgtt
     3721 cacggctcgc gcgacgcgct cgggctcgtc cgcggcgggc ggcgcgggcc ggccgggcgt
     3781 caccgcgcag aaagcgcaac cgcgggtgca ggtgtcgccg aggatcagga acgtcgccgt
     3841 gccgtggttc cagcactccg cgcggttcgg gcagcgcgcg ccgacgcaga cggtgttcaa
     3901 cccctcgcgc gacaggagcc cctggacgcg cttgaagtcc gcatccgtcc gccggtcgga
     3961 ccggatccat gcagggagcc ttggcatggc tggactgtag aaaaggcgcc gggcaagttc
     4021 aatcagatat gaatcaagag cctctccgcg cccggctggc gcggtcgagc cattcgcgct
     4081 cgcggggggt aaggctgtgc aggccgtggt tggcgatctt gtcgagcagg cggtccaact
     4141 cccgcgcgtc gtctgccggc gggggcgtga agagcttcgc cactttccgc cacgagcggg
     4201 cgccgctccg ttcaccgcgg accaggccga gggcataaaa gtagcctgcc aggccgcccg
     4261 cgaggtgcgc ggcgttggcc acgctgccgc tgccgggcga aagcaggaac atcagttcga
     4321 ccaggcccag gatgacggcc agcacccacg cgcgcagggt cacggggacg acgtagaaca
     4381 gcagcagggt gatcggctgg ttcgggaaca gcgcggcgaa cgcgccgagg atcccgaaga
     4441 ccgcgcccga cgcgccgatg catgttacga gcggcgagtc ctcgatccag acccatccca
     4501 gcccgccgag caggccgctg atgaaataga gcagcagaaa cttacgggag cccagcgcgc
     4561 gctccgtctc cggccccagg aaaaacagga cgagcatgtt catgaggaga tgaaacacgc
     4621 cgccgtgcag gaaaatgtac gtcacgggcg tccagaccag gccgcgccgg agggacaacc
     4681 agctcaagcc gaaccatcgc gtgaaggccc cgccggtcag gtcgtcggcg acccattgaa
     4741 aaagcgagag gcccacggtt ccgaacaaca acgcccgcac cgcgggcggg aagttccagc
     4801 gtccgggcgg ggcccaagcg gtcttcgttt cccaaggcat ggttctacca taacgcgggc
     4861 cagcccgatt tcgaatcgaa aacggacttt gcttttttcg ccgaatccgc tctgaatggg
     4921 gtggatgaag ggcctgttca tcacggggac ggacacgggg atcggcaaga ccgcggtcct
     4981 tgccgcgctg ttgtgcgcgc tgcggcgggc cgggatcgac gcagcgccca tgaaacccgt
     5041 ccagaccgga tgccgtcctt cgcgacgcgg cctggtttcg ccggaccttg agcgggcgct
     5101 gcgcgcggcc gggctccggc ctgacgccgc cgagaaaagc ctgatgtgcc cctacccgtt
     5161 ccgccccgcg tgctcgccgc acctggccgc ggcgcaggcg ggcgtccgca tccggctgtc
     5221 cgcgatccgg aaggccttcc gccgcctgtc ggcccggcat tcgtacgttc tcgtggaagg
     5281 ggccggcggg gtgctcgttc cattgaacgg gaataaaacc atgctggacc tgatgcgggc
     5341 gctcgccttg ccggtgatcg tggccgcgcg gccggggctc gggacgctga atcacacgct
     5401 gatgacgctc cgcgaactgc gccgggccgg cctgaaaatc ctgggcgtga tcctggtatg
     5461 cgcccgccgg gctccctggg gcgcgatcga gcggagcaac ctggaaacga tccgacgcct
     5521 cggacgcgtg ccggcacacc gcctacgtta ccgacggggt cccggcgcgc cgggcgatgc
     5581 cgttcgctgg ataaaggagt ggaacctccg cgccagccgc cgcgcgtgag cgcgcctaac
     5641 gcgctggcac gaagcggagc ggccgaaccg tggactcggc ctgttggacc tcgtcggact
     5701 ggcagaagtc gtcgaacaag gcgaccaact gcggcttgag ttcctccggc acgtcctgcc
     5761 ccacgaggta gtgccggcca tgccggccca accgctcggg ctccgccgtc cgcgacacgc
     5821 ccacgcccca gaggaaatcg agccggtgcc cggcggatcg cacgagccat accttggccg
     5881 gtcgatcctt gacgatggcc gccgcctgat ctctgaattg ctgttccgtc atgctgttca
     5941 ttctaccccg ccgcccgttc ttgtgtacag ccggccgtcg ttttccggaa gtcactcccg
     6001 caggaccgca tggatgatcc tgccgacctc gttgaccgtg aacggcttga tcacggcgcc
     6061 cgcgaagccg caggcccggt agttcgacat gcacgaatcg ctggaatagc cgctggccac
     6121 gatggcgcgg acgttcgggt ccagcgcctt gagcttctcc aacgcttcgc gcccgcccat
     6181 ctcgccgggg acggtcaggt ccaggatcac ggcggcgaac cgccggcccg tttccatggc
     6241 ccgccggtac tcctgcacgg cctgcgtgcc gttgcgcgcg gccgtcggtt cgtagccgag
     6301 gtattccagg acgcgcacgg ccacgttgcg cacggcgtcc tcgtcgtcca tcaccaggat
     6361 gcggccggtc ccgtgcggcg ccggccgctc ctcctccggg ggcgcgggca gaatggcccg
     6421 cgaggcgggc agcaggatgt gcacgaccgt gccggcgccc agctcggatt ccagcagcag
     6481 gttgccgccg tgcttgcgca caatggcgta ggaggcggtc aggcccagcc cgctgctctg
     6541 cgccttggtc gtaaagaacg gatcgaagac cttctgcagg tactcccgcg ggatgcccga
     6601 ccccgcgtcc ttgacggtga tgcgcacgta ccggccgggg tccacgggat gagggccgcc
     6661 ctgctccaga tccgcgttct ccacgcgcac gacgatcacg ccgccgccgg gcatcgcctg
     6721 cttgctgttg atgacgacgt tgttcaccac ctgggcgatc tggccgggat ccacctccgc
     6781 ggcccacagg tccgcggaca cgtgcatatc ggcgcggaca ttggagccgc gcaggcagaa
     6841 ctccaccgtg tcccgcacca ggctggacat ggacgtcatc tgccgcacgg gggccccgcc
     6901 ccgcgcgaag gtgagcaact gctggctcaa atccttggcg cgcaggcagg ccttctccgc
     6961 ctcctgcagc agcgccggat gatcgccgcc ggattgcagg gccatgcggg ccagcgagat
     7021 gttcccgagg atgcccgtca ggatgttgtt gaaatcgtgg gcgatcccgc cggccagcag
     7081 cccgagggat tccagcttct gcgcgcgcag ccgctcctcc tccatgcgcc gcagttccgt
     7141 gatctctcgg aaggccacga ccacgccgat gatcttgctg tcgcggtcgc ggatcggagc
     7201 cgcgctgtcg gcgatgatcc gctccgcgcc gccccgggcg atgagcacgg tgttgccggc
     7261 cagcccggcg gtgatcccgg acttgagaac ccgctgggcg ggattatccg cccgcgcacg
     7321 gctcttctcg ttgacgatat gaaagacctc gtccagcgac cggccgacgg cctcggcctg
     7381 cgcccagccc gtcatggtct cggcgacccg gttgatcagc acgatgaggc cggtctcgtc
     7441 cgtggcaatg acgccgtcgc cgatgctgcg gagcgtcacg gcaagccgct ccttctcggc
     7501 ttccagcgcg ctcgcggcct tcttccgctc cgtgatgtcg cgcacgaaca ccacgatctg
     7561 cgcctcctcc ccgatgcgca cgagcctcga actcacttcc acggggaaca gttcgccgct
     7621 gcggcggcga ttgaccgcct ccacgaaaaa gccgcccgtc gtcaattcct cggtgatcag
     7681 cgaaggcagc gtccgggcca cgtcctcggg gaccaggtcg gtcacccgga gccggagcat
     7741 ctcctcccgg gtatagccga acagctgctg cgcggcgccg ttgcagtcga gaatctcccc
     7801 atgcagggtt tcgaggaaaa tggcgtcggt agcggcttcg aagagcgcat ggtaccgctt
     7861 tccgctgtcc tccgagcgcc gggagggatc gggcgttccg gccttcgaat ctggattgtg
     7921 ctgcatcgat gcctcccttt ccccctccgg cggggtctgg cgtatgaata gtaccacatc
     7981 ccgcagtcgc ggaagagcaa aaaaggcggg cggttcgtca gcgcggagcc gacagccatt
     8041 ccttcacgta gcgtgccgcc gcctcttcga ggggcgtgaa cggcttgtcg tacccggcct
     8101 tgcgcagctt caggacgtcg gcctgcgtgt ggtactggta cttctcgcgg agggccgggg
     8161 gcatgtcgat gtaggtgatg cgcggctcca ggcccatcgc cgcgaacacg gccttcgcca
     8221 ggtcgttcca ggtccgcgcc tgccccgtgc cgcagttgaa cagcccggac acgcccgggt
     8281 ggtcgaggaa gaacaaggtg acgtccacgg cgtcctggat gaagatgaaa tcccgcacct
     8341 gctcgccgtc gcggtatttc ggcttgtagg acttgaagag ccggacctcg ccggtttcgc
     8401 ggatctggtg gtaggccttg tgcaccacgg agcgcatgtc gcccttgtgg tcctcgcgcg
     8461 gcccgaacac gttgaagtac ttcaggccga cgatgcggtc gagcagcgcg tggcgcagcg
     8521 cccacaggtc gaacatgtgc ttcgagtagc cgtacatgtt caggggcttg agcgtcaggg
     8581 tgtgccggtc gtcgtcggaa tagccgctct cgccgtcgcc gtacgtcgcc gcgctggagg
     8641 cgtagatgaa gcgcgcgccg ttgtgcaggc accactcgca gagctcgcgc gtgtaccggt
     8701 agttgttgtc ggccaggaag cccgcgtcct tctcggtggt cgaactgcac gcgccgagat
     8761 ggaagaccgc gtcgacggcg gtcagcgcgt cgtgctgaat ggccgctcgg aagtcgtcgc
     8821 gccccatgta gtcttcgtaa cgcaggccga ccagattctt ccacttctcg tccgtcccca
     8881 gttcgtccac gaccaggatg tggtcctcgc cccgcgcgtt gagcgccgcc acgaggttgc
     8941 tgccgatgaa tcccgcgccg ccggtgacga tgaacttggt gctcatggct tggcctcccc
     9001 ctcctccttc tccctctcct cctccccctc atttcccccg ccggcccccg ctccgtactc
     9061 cgctcggtcc tcgcgaatca agcgggtttc cgagaaatac gcgacaagtt tggacagcat
     9121 ggctgcaatg ctcgccaaca agaccttccc gtcctccacc ctctccgcgg ggaccgcgcc
     9181 ctttaccgcc agggcatcca ggcatgccgc gcactcggcc gccgagccgc gggcatcgtc
     9241 aaagaacttg gccctcatcg gcatccgcct ccgcccgttt ccctcggcgg tattcaacaa
     9301 catcgaaagt cccgcccggt ccaattgatt gcgcacttcg cccgtgcagg gaccatcgcc
     9361 ggacttcaat tcgttgagca aaacggttga ccagcgaaca aaatccaact ccaaccggta
     9421 gacatcgagc ttttcatgag aaaaacgcgg gttcatgagt catgaggggg agaaggagta
     9481 ggagtaggag aaggagggag agaaggagag ggggaatggg cttggaggat cttcccgatc
     9541 atcccgctgg tggaccgccc ctcgaccatc ggggccagca ccacacggcc gccggctttc
     9601 tccacgacgt cgcgcccgct gacataatgc gcccagtcgg ctcccttgac cagcacattg
     9661 gggaccaggt ccgcgatcaa gtccgccggc tcgtcctcgt cgaagaccac cacgtaatcc
     9721 acgcattcca gcgccgccag cacgcgggcg cggtccgcct gcccgttgac cggccgcttg
     9781 tcgcccttgt aacggcggac cgacgcgtcg gagttcagcc cgacgaccag cgcgtcgccc
     9841 tgctgcctcg caaattccag gtaggtcacg tgccccgcgt gcaggaggtc gaacgcgccg
     9901 ttcgtgaaga cgagtttctt ccccgccgcg cggagccggg cgcgctcggc ggccatcgtt
     9961 tctctcgaca ggatcttgga ttccgcgggg cgcatgggag ggttcagggt tcagggttca
    10021 gggcggtcac gacatggcct ccgcgaccag ccggccgaat tcgctgcatt tcacctcacg
    10081 ggcgccgtcc atgagccgcg cgaagtcgta cgtgaccgtc ttcgcccgga tcgcctgctc
    10141 catgccgcgg acgatgaggt cggcagcctc ggtccagccg aggtagcgga acatcatctc
    10201 gccggacagg atcagcgagc cgggattgac cttgtcctgg ttcgcgtact tcggcgcggt
    10261 gccgtgggtc gcctcgaaga ccgccgcgcc ggtgtcgtag ttgatgttcg cgccgggcgc
    10321 gatgccgatg ccgccgacgc aggcggccag cgcgtcggag atgtagtcgc cgttcaggtt
    10381 cagcgtcgcg atcacggagt actcggccgg ccgcgtcagg atctgctgga ggaacgcgtc
    10441 ggcgatgaca tctttcacga cgaggtccgc gccggtgcgc gggttcttca ggcggtgcca
    10501 cggcccgcca tccagcggct ccgccccgaa ctcctcgcgg gcgacccggt agccccagtc
    10561 gcggaacgcg ccctcggtga acttcatgat gttgcccttg tggaccaggg tgaccgacgg
    10621 gcggccggcc tcgatcgcgt agcggatcgc ggcgcgcacg agccgcgccg tgccctcgcg
    10681 ggagacgggc ttgatgccga tcccggacga gcccgggaac cggatcttct tcacgcccat
    10741 ttcgcgctgg aggaaatcga ccaccttctt gacttccggc gtgccggcgg cccactcgat
    10801 ccccgcgtag atgtcctcgg cgttctcgcg gaagatgacc atgtcggtgc gttcgggatg
    10861 cttgaccggc gacgggacgc cctcgaacca gcgcaccggc cgcaggcaga cgtagaggtc
    10921 cagttcctgg cgcagcgcga cgttgaggct gcggatgccg ccgccgaccg gcgtggtcag
    10981 cgggcccttg atggacacct tgaactcgcg gcaggcctcg agcgtctcgg gcggaagcca
    11041 gaccggttcc ccgtagaccg cgttggcctt ttcgcccgcg tagacctcca tccattccac
    11101 gcgccgccgg ccggcgtagg ccttctccac ggccgcgttc cacgcggtca tggccgcccg
    11161 cgtgatgtcc ggcccggttc cgtcgccctc gatgaagggg atgatgggac gatccggcac
    11221 ctgaaggcgc cgatccggcc ccaacgcgat cttccggccc gacgccggaa tctgtatccg
    11281 gtcatagtgc atagcttcca ttcctgcccg aaacgggttc gtttggccag aagttttcgg
    11341 ccggcgcgat tcttctcttt acaggacggc cggggcgctt atattccatg aggtaaatat
    11401 ttcgtgcaac taaataataa acggatcggg gacatgacgt cgtcgcagga cgcggatcgg
    11461 ctgatggagc tgctgcccaa gctgatgcgc gggatccatg cctacgagcg gaattttttc
    11521 acctgcgaga acatcacttt tccccagctc tgggcgctcg atttcgtcca cgagcatccc
    11581 cgctgcacga tgcatcaact ggccacggcc atgcacagcc gtcaatccac ggccacggga
    11641 ctcgtggacc gaatgcagcg gctcaacctg gtgcggcggg agcgccgccg cagcgaccgc
    11701 cgcgtggtct gcgtttctct ctccgaaaag gggcgctcga tggttcggca gatcaccgac
    11761 cagcggcgcc ggatgatccg gcgtctgttc agccggatga gtgaacggga ccggaggcag
    11821 tacctcgaca ttctcgggcg gctggtcgag cagttcagcg aaccggcccg ggcccggacg
    11881 ggaggccatc cgtgagacgg ctcccgatcc tgatggcggg cctgttggcc ctgcgaccgt
    11941 gcttcggaca gaccaacacg gaggccccgc tgaccatcga gcgggccttc gagatggctc
    12001 tggagcgtaa cgaggaggcc cggctggcgg acctggccgt gaaacagcgc gcgctcctgc
    12061 cctggcgatc cctctccctc ctcttcccct cgctcacggc ggacgcctcg cggatgattc
    12121 cggaggatga aatcgtagga gagagcggga gccccgtcgt cgcggacccc acggaccggg
    12181 ccagcctccg tctgtcgcag cccctgttca gcagtgacgc gtacgcgggc taccggagcg
    12241 gccgttccct ggaacgggcg gcgatcgagg accgcgtctt ccggctccgc gacctgctct
    12301 tccgggtggc cgccaccttc atcgactgcc tcaagctgga gcacgtggtg ggcatcaacc
    12361 gggagacgtt gactctggcc gaggaacagc ggcggacggc cgaggcgcgg tacgaggcgg
    12421 gcgaggtgcc gaagacggac gtcctgcgcg cggaagtgga agtcagccgg gcgcggcgcg
    12481 aactggacgt gtcgcggaac cagtggcaac tcgcgggcgc gcagctcgcc acgctgatcg
    12541 gcgcaggcga cggcccggtc tccgtgcagg cgcccccgga ggggctggcc gaccgactgg
    12601 aacacgcgga ctcgctggag accttgatcg cggcggcgct ggaacaacgc gacgacctgc
    12661 gacgggcggc ctacgagcgg gaggcggcgc gttggtcgct ggtcaaggcc cggcggggct
    12721 ggtggccgac ggtcagtttt gacgtggagc agacgtgggc cgacccgggc agcctgtccc
    12781 agccggacga tttctggacc gccacgttcc gcgccagcct tccgctcttc gagggcggcg
    12841 cgcggcagtt ggacgcccgg gaggcgcggt acgccctgga ggaggcggat ctcgtggtca
    12901 gccagctcgt gaagaccgtt cgcctccagg tgacggaagc ctggctgcgc acccggacag
    12961 cccgggtgtt gctggacgcc acccagaagg agcttgaact ggcccgcgag aattatgacg
    13021 tggtgtccca gcgctatcgc gccggcaagg cgacgagcct ggaccaggtg gacgccttca
    13081 cccagctctc gcgggcgcgc acgagcgtgg ccaacctgac gtacgaccac caggcggcga
    13141 tccttcactt gctccggcag gtggggtcgc tgggcgagcg ttacgtggcg gatacccgga
    13201 gcgaaccgga ccgctccgcg cgcgggagga cggaatgaat ctgaagtcac agtggggcgg
    13261 gccgtggctg gcggtcctgc tggcctcgac ggcgggagcc cagcctcccc cgggcggcgg
    13321 acgggaggcg gccggggaac cgattcccgt cagggtcatg cgcgtggagc cgcgcgacct
    13381 gcagcaggcg ctggaatacg tcggggacat caaggcccgc gacgaagccg tggtgtatcc
    13441 gacggtcacc ggcaagatcg cggaaaagct caaggaggac ggcgcgcggg ttcggaagga
    13501 tgaagccctc gccctcgtgg accgcgacga ggtcggtttc aagttcgaga aagcgccggt
    13561 gaagagcccg ctcggcggga tcgtggggcg cgtgtacgtg gatctcggcg ccaacgtcac
    13621 gccccagacg ccggtggcgc tggtggtgaa catggaccag gccaagatca acctcgacat
    13681 cccggagagg tatttgcccc gcctcgcgcc gggccaggtc gcgctgatca ccgtggacgc
    13741 ctacgacggg attttcaccg gccgggtctc caagatcagc ccggtcgtgg acctggccac
    13801 ccggacggcc ccggtggaga tcctcgcgcc gaaccgggcg cacgaactca agccgggcat
    13861 gtttgcccgg gcgcgcctgc tcatggagga gcgcaagggc attctcgtcg tgatgaagga
    13921 ggcggtcctg gggatggcgc ccgaccagta cgtctacgtt gtcgaggacg acacggcccg
    13981 gcagcgaccg gtgcggctcg gcctgcgcga ggcgcacgag atcgaagtgc tggagggcct
    14041 gaccgccggc gaggcggtgg tgatcgtggg ccagcagcgg ctgcgcgacg gcctgcccgt
    14101 ggccgtcgaa gaggcggcgg ccccgtaggc acggagcgct ccatccatga accttcccga
    14161 attcggagtc agacggcccg tcaccaacct gatgatcttc ctcgggatca tcatcctttc
    14221 cctcttcagc ctctcgcggc tcggggtgga catgatgccg gagatcgagc cgcccgcgat
    14281 ctcggtcgtc tccctgtatc cgggcgccag cccggaggac gtggaagtca aggtcacgga
    14341 gccgctggag aaccagctcg ccagcacgcc gcacctggag aaaatcacct cgcgctcggc
    14401 cgagggcgtt tccgccatcc agctcaagtt cacgtggggc gtcaacctcg acgaggcttc
    14461 caacgacatc cgcgaccgca tcgaacgcgc caagcgggcc ttgccggaca tcccggacga
    14521 gatggacaat ccgttcatct tcaagttcaa caccgccttg atgccgttcc tgatcctggg
    14581 cgtcacggcg gaccagaata cgccggagct gaacgacctc gtcgaccggc gcgtggcgga
    14641 ccagctccgc cagttgcccg gcgtgggtac cgtccaactc cgcggcgggc tggaccggca
    14701 gatcaacgtg tggatcgatc gcggccgcct ggaaggctac ggtttttcca tcctggacgt
    14761 gaaagcggcg ctcgccgcgg cgaacgtgac gcagccggtg ggcaacatca agtccggcct
    14821 gacggactac ctggtccgcc tgcccggcga gtttgccagc ccggacgaga tcaactcggt
    14881 catcctcggc caggaccagg gccgcctcat ctacctcaag gacgtggcgc gggtggagga
    14941 ttcccacgcg gaggtctcca gcgtcgtccg catcaacggc aagtcgggac tgatgatggc
    15001 cgtgatgaag cagaccggcg tcaataccgt cgaggtcgcc gaccgggtga agaaaaaact
    15061 ggcggagatc gagccttccc tccctctcaa catccggttc cacatcatga tggactcctc
    15121 ccaggacatc ctggacgcgc tgtcctccct gcgcgagacg gtctggatcg gcggggccct
    15181 ggtcatcctc gtgacctggt ttttcctgcg ccgggtgcgg cccagcctga tcattgccct
    15241 gacgatcccc ttctcgctgc tgatcgcctt catctacctc ttcttgagcg ggcggacgat
    15301 caacatcatc agcctctcgt cgctgaccat cgccatcggc atggtggtgg acaacgccgt
    15361 cgtggtggtg gacaacgtga tccggcgcct ggaccagggc gaacggtctg cggaggcctc
    15421 gattttcggc acctccgaaa tgtacctggc catcgccgcc tccacgggca cgacggtcgt
    15481 ggtattcctc cccctcctct tcgtcagcgg ggtcgtgggc atcatgttcg gggaactggc
    15541 ggtgatcatc acggtgaccc tcctggcgtc gctgttcacc gcggcgacgt tcagcccgat
    15601 gctgtgctcc aagtggctca agcccgcggc gcgagacggc gaggccgcgc ggggccggct
    15661 gggctcaagg gtgtacgaac gcttcgagcg ctggtttacg gcctgggagc gggcctacgc
    15721 ccgcgtcctg cactggagcg tgcgccgggc ctggcaccgg gttctggtcc tgttcggatt
    15781 tacggcggcc ttcgcgggaa gcctgctgct cgctccgcgg gtcggcacgg aattcatccc
    15841 ggaggaggat agcggcgatc tccgggtgag cctgcaactc ccggtgggca cgcgcctcga
    15901 ggagtcggac gccgtcggcc gcaaggtcga ggcgatcttc ggggcggagg tgcccgagat
    15961 gcgtttcatg tacatgcaaa gcggccaggg cggcggaatg ggcgcggcgt tcgggggggc
    16021 gtcgggctcc cattccatat ccagcggcgc caggctggtc cccaaggagg agcggacgcg
    16081 gtcctcccgc gagatcgccc aggccgtgcg ccagcgggtg cgccgcctcg ctggcgtctt
    16141 gaggaccgac atctatgcgg gcaacccgat gggccggctg atcacaggca ccggcggcaa
    16201 gaaggtccag atcgaggtca tcgggcattc attcgaggag acggacgccg tggccgaccg
    16261 gatccgggcc gcgctcgagc aggtggaggg cgcggtggac atcagcgtct cgcgtgaaat
    16321 gaagcggcct gaactgcgca tcgccgtgga ccgggagaag gcggcggcgc tgggcctgga
    16381 catgcaaacc gtcgccgaga cggtcaccac cgccatcgag ggctccacgg ccacgcggta
    16441 ccgcgagcgc ggagagacct acgacatctt cgtgcggctc gaggaggcct ggcgctccaa
    16501 gccggaggat ctggagagcc tgaccgtcct gtcgccctat acccgccgcc tggtgcgcct
    16561 ggcgaacatc gcccgggtca tcgagacccg ggggccgctg gaaatcgagc ggcagaaccg
    16621 cgagcgcgtg gtgcgggtcg aatgcaacac gcacgaacgc tccctgggcg acgtggtcgc
    16681 cgacgcgcgc cgggccgtgg ccggcatgac gctgccgccc gacgtgctca tcaactgggg
    16741 cggcgaggcg gaggagcagg ccaaggcgtt cggcgatctc acgctgctgc tgctgttggg
    16801 catcgcgctc gtgtacatgg t
//
LOCUS       DAOOTK010000064        16507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2408_length_16507_cov_5.094639, whole genome shotgun sequence.
ACCESSION   DAOOTK010000064 DAOOTK010000000
VERSION     DAOOTK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_16507_cov_5.094639"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK634_08445"
     CDS             complement(<1..186)
                     /locus_tag="PK634_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97802.1"
                     /translation="MLRAFAALAMAGAFTVTAYALSLRLAGQGSALRRLCATVIVAMA
                     LATILFHALIGCGQFRLA"
     gene            complement(206..1378)
                     /locus_tag="PK634_08450"
     CDS             complement(206..1378)
                     /locus_tag="PK634_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOW97803.1"
                     /translation="MGNLGAEAITLAVIPFFQERWGMETRFILAAWDPARMTELLRGL
                     PGELEVIKQTVPFDRPGSLRRSDMFVVCGDVALTETVIPILPCYWAFKSWWARLFGCR
                     VLFLGVDAEPMRRRLNRWAIRLALDRIVEHYLVRNDEARRHLLGVSRRPDRVLLGCEP
                     ALLIEDRYLSLFPPPAFDRKGARRIVGLGIRDFFSEPLRLDPFRLKLVRRDAAPGQLS
                     DRMKKTVAGLARIADRLAERHDARLFVVPHHALDESKKVILSDREVAEHLRRAMRNPD
                     RLEVLPENLHPYAAMNFYRQCDLAVSMRHHTSSFAFRFGVPVIGLGVSEKIRGHFRLV
                     EQPELLLDPAAPGDAMDRVVDDAVARRDELSGRLRRKLPELQADMKRALDAAIRND"
     gene            1554..2519
                     /locus_tag="PK634_08455"
     CDS             1554..2519
                     /locus_tag="PK634_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW97804.1"
                     /translation="MEPLLEVRGLRVCFGPADRPARAVDGVSFRVDAGETVALVGESG
                     CGKSVTALSLARLVPEPPGWYAGGEIRFVGTDLMRLRPAGLRGVRGREIAYVFQEPAD
                     ALNPLFTIGAQVMEAIRLHRPDEEAQSIAASLLEKVGLPAPEALMRAYPHQLSGGMQQ
                     RAMIAMALACRPRLLVADEPTTALDVTIQAEIMDLLQNLQAELGMAILLITHNLGIVA
                     RIAQRVYVMYAGRIVESGPVRNVLDHPAHPYTRALLDAVPRLTGSRARVSGIAGSVPD
                     PADWPAGCRFHPRCPRAQALCRERLPAVEEAGPGRSVACHFWRDA"
     gene            2516..3283
                     /locus_tag="PK634_08460"
     CDS             2516..3283
                     /locus_tag="PK634_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW97805.1"
                     /translation="MTPLCEVSALRVQYRRGKEVVRAVDEVSFEVGRGETVGLVGESG
                     SGKSSLGRALLRLEPIHSGRVLFEGADVAGLHGPALKAFRKRAQILFQDPYGSLNPRI
                     TVGRTIEEVLIVHRWAGDRAARVAELLELVGLPGSSARRYPHEFSGGQRQRVAMARAL
                     AVEPVFLVADEALSALDVSVQAQILRLLIELKEKLGLAYLFISHDLAVVRSIADRILV
                     MREGRILESAPTDRLYESPSHEYTRTLLAAVPDPFRK"
     gene            3331..4470
                     /gene="tgt"
                     /locus_tag="PK634_08465"
     CDS             3331..4470
                     /gene="tgt"
                     /locus_tag="PK634_08465"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOW97806.1"
                     /translation="MTPGTFEILQQAPGAGARLGRLWTAHGPVDTPVFMPVGTQATVK
                     AMTPAEMEDLGFQIVLGNTYHLNIRPGVDIIEEQGGLHRFMGWERAILTDSGGYQVFS
                     LARLNRITADGVIFQSHVDGATHVLGPESAMAIQRRLGSDIAMVFDECAPYPCDRKYA
                     CQAVERTLAWAALCARQPRAAGQLVFGIVQGSVYADLRERCARGLIELGFDGYAIGGV
                     SVGEPHALILQGIEDSVRHLPADRPRYLMGVGSHQHMVEAVARGVDMFDCVMPTRLAR
                     HGTAFTRAGKYPVKAALYARDPRPIEEGCGCYACRHFSRAYVRHLMNVGEILGIRLLS
                     IHNLFRYEAFVQEMRGAIRAGGFAEYAKGFVEQYGNGRDQPGRDE"
     gene            4483..4839
                     /gene="yajC"
                     /locus_tag="PK634_08470"
     CDS             4483..4839
                     /gene="yajC"
                     /locus_tag="PK634_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOW97807.1"
                     /translation="MNFLMTWLAMAPAPGGTGGQQQSPAFMIGWLGLMLVIFYFMLIR
                     PQQRREKERRALLTQIKSGDRVVFCGGLLGIVTNAKEKTFTIKVDEGVKLEVVRSAVT
                     QVLEKGETPEDSPAKK"
     gene            4881..7367
                     /gene="secD"
                     /locus_tag="PK634_08475"
     CDS             4881..7367
                     /gene="secD"
                     /locus_tag="PK634_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOW97808.1"
                     /translation="MDKHSLWKWLVLIGLVAWSMVLVMPLHEKVKLGLDLRGGMSFVL
                     EVDTTTLAPDAISDAQARALEVIRGRVDAMGVAEPIIYPEPGNNRIVVQMPGLGAEDR
                     DRAVRNIQSAAFLEFRMVHPKNDDLVEDLFDQKLVPDGYKIVSIDEQMPSGQWKAVNY
                     YKRDVSLDTPGTTDREVRDRLKLFKPQSNYDFMLMKEIRNGQELFQPYYVRRLAELKG
                     EDLIRAGVDFRPPLNQPIVTLKFDAKGSRRFAKLTSDYAPGGQLNPGMDSRRYLAIVF
                     DGQLRSAPFIKTAIHGGEAIIEGSFSVKEAQDLSIVLRAGALPAPVKVMEERRVDPSL
                     GSDSINSGKRAAIYGGIGVVVFMCIYYLGAGLVANLALVLNLVLLPFGMWLVAGFFSI
                     LETRGLGGGGMISLPTLTLPGLAGIVLTAGMAVDANVLIFERIREEQAAGKRFAAAIP
                     AGYDKAFSAILDSNLTTLLVAIILFWQGSGPVRGYAVTLSAGIVVSMYTAITVTRMIF
                     TTLAQRFDIGSMKMLQFFKSTKINFLRWRFVAGGLSLTIIIVTWVLFVLRGQDNFGVD
                     FTGGSSITFQFEEKRPEETIRQLLESEGIEDPFIQYQRLVTAESGGTEVEYLEVKTPF
                     ADGDKTASVITEKLADAKYSVLKQDSIGPQIGQELKRKGLKALILSFIVMIIYVSWRF
                     EFGYAIGALLALVHDVLVTVGIYTLLGRQLSVTTLAAVLTIVGYSVNDTIVIFDRIRE
                     QLKLNRGMKYADVANMSINQTLSRTVLTSGTTMITVLALLLLGGGAINDFALVMFIGM
                     IAGTYSTIYMATPIALLWHREKPREDEKKA"
     gene            7438..9126
                     /gene="recJ"
                     /locus_tag="PK634_08480"
     CDS             7438..9126
                     /gene="recJ"
                     /locus_tag="PK634_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOW97809.1"
                     /translation="MPKLWKTVERVGREADPLAASLGIPRPLAEVLATRGLAEPVEAR
                     RFLNPRLDDLSDPTVLPDMPRAVDRIRRAAKGRERIAVFGDYDADGVTAAALLTRCLR
                     ELGAEAEPFLPLRLEEGYGLGVDALNRCLEAHRPQLVITVDCGTGSVDAVRAAAAAGV
                     DVVVTDHHEPAGEIAPAIAVVNPKLGDGGDLANLAGVGVAFKLAWALALRSGVDIRRI
                     FRRLDLVATGTVADVVPLTGENRILVHYGLGQLNREPLPGLAALAEAAEVKDAIGEYH
                     LGFAIGPRLNAAGRLGDAQAALELLLGDDPEALLPLARQLDRGNRERQKIEADILEEA
                     MQDVAALDLENNAGLVLARRGWHPGVIGIVASRIVQRFGRPVLMISLNEDGTGRGSGR
                     SIEGFNLVENLAACRDSLVRFGGHAMAAGLELKEKNLAAFREAFNRRVSETVPPDQLR
                     PVQRVDAWLDLADADERLFEGLQALRPFGCGNPRPVWASSRVRVLGQPRILKDKHLKM
                     RVVSGSAQREAIGFNLAGQPLPDGPMDIAFTLDMNSYMGRETLQLKLEDFRPSP"
     gene            9196..10167
                     /locus_tag="PK634_08485"
     CDS             9196..10167
                     /locus_tag="PK634_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97810.1"
                     /translation="MSVGALLGLLLLQPCFAGTREERLAVVQFIHPGSEHEPDSADGR
                     SWNTDAHRRKFLLQPGRYLASLESKPVAADLVFWGEFEPPTRLVRTFPETGGDMPRHL
                     FTPDPVAFYPHDPPLMNTDPFVFGGRFFYSICKQNNRRGPTALQRLARGSVILFGSGR
                     GRDRFVVDTVFVVADYVDWNLSNYRERLKDVVPPEYFHSTLEPIAYEMQVRNLTPSQT
                     FRLYIGATFDRPCEGMYSFFPCRPYRQEANTGFARPVLRRPGIITDNLTQGQRLNPMP
                     DAATVREIWEDIARQVLGQGLDLGVHAEMPRIRQVGRLGPSRRDTAR"
     gene            complement(10179..11054)
                     /locus_tag="PK634_08490"
     CDS             complement(10179..11054)
                     /locus_tag="PK634_08490"
                     /inference="COORDINATES: protein motif:HMM:NF037498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA base hypermodification protein"
                     /protein_id="HOW97811.1"
                     /translation="MNSAANPYRDALRGLELADPVRAFFDWCRERERIREKRERGEPP
                     QWSADPIFQRGRFLNVFREDDRGSRAVVRFVRRAGSSLPELLHALFFARWCNRDTTLD
                     AMEPPLLRQPARLRHFLLHETAQPWFSEAYPVVEARWENRTYDRLEGGIELIPRALGF
                     LEACVRGAAGHVVTATRRINAEFRMSNDFPIFMAVADIAWFDPSLIRPDSPVPTGIGS
                     APFMDLLQKHLGLPDHEATMAKMIELQSVYWPEARRAFTPIDVEYLCCECRKYYSYVN
                     GTKRFEGRNLFTPNR"
     gene            complement(11051..11836)
                     /locus_tag="PK634_08495"
     CDS             complement(11051..11836)
                     /locus_tag="PK634_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOW97812.1"
                     /translation="MSLQVCVLASGSSGNCIFVGSTRTGILIDAGLSGKETLRRLDLL
                     EIPAARIRAVCVSHEHNDHTAGLRALQRRHGLDLYANTGTIEALQRNPDLNDLAWKVF
                     STGFAFSIGDLTVEPFAVSHDAYEPVGFVIAAGRERVGIATDIGVATGLVRERLKGCR
                     VLVIESNHDERLLLEAERPWQLKQRIAGRQGHLSNASAARLVCELAGPELEHVFLVHL
                     SEECNRQDLALKTARQMLDQAGHARIGVSLTFPDRVSEIWCSA"
     gene            11966..12973
                     /locus_tag="PK634_08500"
     CDS             11966..12973
                     /locus_tag="PK634_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HOW97813.1"
                     /translation="MSNAQTFPTILVTGGGGYVGSALVPALLKQGHKVRVVDTFWYGE
                     NVFADCNSHPALTRIAKDIRDTAAMKEAMRGVDAVIHLACISNDPSFELDPKLGKGIN
                     LDAFPGLVEAAKAGGVKRFIYASSSSVYGVKEQPDVREDDPPAPLTDYSRFKLECEKL
                     LMKLGGGMTRVIVRPATVCGYAPRLRLDLTVNILTIHALVNRKIRIFGGRQMRPNLHV
                     LDMVRAYEMFLAAPAEKIDGVPFNVGFRNETVENIAGIVKRTIGDEGIALEYVPTDDN
                     RSYHVNSDRVRRVLGFEPKYNIEDAVRSIVEAYRAGKIPDPMTNPMYHNIKRMHQLSL
                     G"
     gene            12985..13602
                     /locus_tag="PK634_08505"
     CDS             12985..13602
                     /locus_tag="PK634_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOW97814.1"
                     /translation="MHLGVDFDNTLVSYDELFHRCALERGLIPADLPRTKSAVRAHLW
                     ALPDGNTPWTELQGLVYGTRMAEAAFFAGAREALVLCRERGIRVSIISHKLEFPARGP
                     RVSLRKASLEWMEAQGFFDPAAIGLAREAIFFESSREAKLARIAGERCTHFVDDLPEV
                     LDAPGFPAAVEKWFFDPTGSAKWPAGARRFGSWAEIRSHLETMRP"
     gene            13599..14654
                     /locus_tag="PK634_08510"
     CDS             13599..14654
                     /locus_tag="PK634_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOW97815.1"
                     /translation="MIPDIPKEILSALERMLGRAPERVTRLPGGANNLVLEIVLGHSR
                     YLAKAYFQHKNDPRDRLGAEFGMLTFLWRHGVREVPEPLQSDPARKIGIFEYVDGMRL
                     APGEVTAPDVRQLVDLLTAMWRLRGESDAQDMAPASDACFSIRGYMENVGGRFQRIRN
                     ALEQGGAVLEAEAYIRGDMARTWERILDFMEQGAKEAGLDAALPLERAEQTLNPADHG
                     FHNTLREANGRLRFLDFEYSGWDDPAQMICNALWQPAVPLPDRQRKPFLKRVFKGLEG
                     DAALAARSRLAYPILGFKWACIMLNEFLPVSEERRRFAGTRPEDRRREQLEKSRRRLM
                     DIEDYLAAPSLFDGIAF"
     gene            14703..15512
                     /locus_tag="PK634_08515"
     CDS             14703..15512
                     /locus_tag="PK634_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOW97816.1"
                     /translation="MDQRALEQRLEILKILEFSRRGHIGSTFSLLEIVRVLYDDILRI
                     DPKNPLWPDRDRFVLSKGHGSLSLYVNLAEKGFFPKDELFRFCKFNGILGGHPEYGVV
                     PGVEASTGALGHGLSIAVGMALAARIDRKGYKVFALMGDGECDEGSVWEAALSAGKHK
                     LSHLTAIVDYNKMQSYGPIDEIQSLEPFADKWRAFGFAATEVDGHDVDALRALFKSLP
                     LDPGKPTAIIAHTVKGKGMSCIEGNPSWHHKTKITDAEFDQLYADLGFPRK"
     gene            15509..16432
                     /locus_tag="PK634_08520"
     CDS             15509..16432
                     /locus_tag="PK634_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004777170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOW97817.1"
                     /translation="MRKVCLKSMAKLAAMDERVVFIGSDITKRDLEDFSDNFPSRFFM
                     EGVYEAHIVGMAAGMALCGKMPYINTIATFLTRRCYEQVLLDAGLHKLPVRLLGSGGG
                     VVYAPLGPTHLANEDIAILRAIPHMCIVAPCDAEEMARLMPLTLKWPGPMYIRFAKGG
                     DKVVSRPDLPFEIGKGILMKDGSDVLLVTTGITTQIALEAAATLAGEGIGAAVLHMHT
                     VKPLDAEQLIALAGRARAVVTAEEHTIIGGLGSAVAETLLEASVAKPFKRLGFPDVFT
                     EELGSQNDIMGKYGISVDGLCSTAKTLLKIS"
BASE COUNT         2818 a         5326 c         5626 g         2737 t
ORIGIN      
        1 cgcaaggcgg aattgcccgc acccgatcag ggcgtggaag aggatcgtgg ccagggccat
       61 ggccacgatc acggtcgcgc acagccggcg cagcgccgag ccttgtccgg ccagccgaag
      121 ggagagggca tacgccgtga ccgtgaaagc ccccgccatg gccagggccg cgaacgcgcg
      181 aagcaagatc ccgatcctgg aggggtcaat cattccggat ggcggcatcg agggctcgct
      241 tcatgtccgc ctggagttcc gggagcttcc gtcgcaagcg cccggaaagc tcatcgcgac
      301 gggcgacggc gtcgtccacg acgcggtcca tcgcatcccc gggggcggcg gggtccagca
      361 gcagctccgg ctgttccacc aggcggaagt ggccccggat cttctcgctg accccgagcc
      421 cgatgaccgg cacgccgaac cggaacgcga acgaactcgt atggtgccgc atggagaccg
      481 cgagatcgca ctggcgatag aagttcatgg cggcgtaggg atgcaggttc tccggcagca
      541 cttccagccg gtccggattc cgcatggccc ggcgcaggtg ctcggccacc tcgcggtcgg
      601 agagaatgac cttcttcgat tcgtccaggg cgtgatgagg cacgacgaac aggcgcgcat
      661 cgtgcctttc cgccaggcga tccgcgatgc gcgccagtcc ggccacggtc tttttcatcc
      721 ggtcggacag ctgcccgggc gccgcatcgc ggcggacgag tttcagccgg aacgggtcga
      781 gccgcagcgg ctcggaaaag aaatcgcgga tccccaggcc gacgatccgg cgcgcgccct
      841 tccggtcgaa ggccggcgga gggaagagcg acaggtagcg gtcctcgatc agcagcgcgg
      901 gttcgcaacc gagcaggacg cggtcggggc ggcgtgaaac gccgagcagg tgccggcggg
      961 cctcgtcgtt gcgcactagg tagtgctcca caatccggtc gagcgcgagc cggatggccc
     1021 aacggttcag gcgccggcgc atgggctcgg cgtccacgcc gaggaacagc acgcgacagc
     1081 cgaacaggcg cgcccaccac gatttgaagg cccagtagca gggcaggatc ggaatgacgg
     1141 tctcggtcag ggccacgtcg ccgcacacca cgaacatatc cgagcgccgg agcgaaccgg
     1201 gccggtcaaa ggggacggtc tgcttgatca cctccaactc gccgggcagg ccgcgaagca
     1261 gttcggtcat gcgcgccggg tcccaggccg ccaggatgaa ccgggtttcc atgccccagc
     1321 gctcctggaa aaaggggatg accgcgagcg tgatggcctc ggcgccgaga ttgcccatgc
     1381 ctccggcgcc ggagaggaat accgttttgg cggggcggtt cattgaaagt cggcctcttc
     1441 atactggagg tgtctgggca tgtcaacggc gcaccggcga cgctgaaaaa actggttgaa
     1501 cggggacgtt cggcctagag tacgcgcccg tcggaaaacc gggtatggca tccatggaac
     1561 cattgctgga agtgcggggc ctgcgcgtct gcttcgggcc ggccgaccgg cctgcgcgcg
     1621 ccgtggatgg cgtgtcgttc cgggtcgatg ccggcgaaac ggtggcgctg gtcggggaaa
     1681 gcggctgcgg gaagtccgtc acggcgctgt cgctggcccg gcttgtgccc gaaccgccgg
     1741 gctggtacgc gggcggcgag atccggttcg tggggacgga cctgatgcga cttcgtcccg
     1801 ccggattgcg cggggttcgc gggcgcgaaa tcgcgtacgt cttccaggag ccggccgacg
     1861 cgctcaatcc cttgttcacc atcggggcgc aggtgatgga ggcgatccgg ctgcatcggc
     1921 cggacgagga ggcgcaatcc atcgcggcgt cgctcctcga aaaagtcggc ctgcccgctc
     1981 ccgaggcgct gatgcgcgcc tatccccacc agttgagcgg cggcatgcag cagcgcgcga
     2041 tgatcgccat ggccctggcc tgccggcccc ggctgctcgt cgcggacgag ccgaccacgg
     2101 cgctggacgt gaccatccag gcggagatca tggatctgct ccagaatctc caggcggaac
     2161 tcggcatggc gatcctgctc atcacgcaca atctcggcat cgtcgcccgc atcgcgcagc
     2221 gcgtctacgt catgtacgcg ggacgcatcg tggagtccgg cccggtgcgg aacgtgctgg
     2281 atcatccggc ccatccgtac acgcgggcgc tgctggacgc cgtgccccgg ctgaccggga
     2341 gccgcgcgcg ggtgtcgggc atcgcgggct cggtgccgga tccggcggac tggcccgcgg
     2401 gctgccggtt ccacccccgt tgcccccgcg cgcaggccct gtgccgcgaa cggctacccg
     2461 cggtcgagga ggccggcccg gggcggtcgg tcgcgtgcca tttctggagg gacgcatgac
     2521 gccgctctgc gaggtcagcg ccctgcgcgt gcagtaccgt cgcgggaaag aagtcgtccg
     2581 cgcggtcgac gaggtcagct tcgaggtcgg gcggggggag accgtgggcc tggtgggcga
     2641 gagcggcagc ggaaagagtt ccctgggccg ggccttgctg cgcctggagc cgatccattc
     2701 cggccgcgtg ctttttgaag gcgccgatgt cgcgggcctt cacggcccgg cgctgaaagc
     2761 attccggaag cgggcgcaga tccttttcca ggatccgtac ggttcgctca acccgcgcat
     2821 cacggtgggc cggacgatcg aggaggtgct gatcgtacac cggtgggcgg gcgaccgggc
     2881 cgcgcgggtg gccgagttgc tggaactcgt cggcttaccg gggtcctccg cgcggcgcta
     2941 cccgcacgag ttcagcgggg gacagcgcca gcgcgtggcc atggcccgcg ccctggccgt
     3001 cgaaccggtt ttcctggtgg cggacgaagc gctctcggct ctcgacgtgt ccgtgcaggc
     3061 gcaaatcctg cgtttgctga tcgagttgaa ggagaaactg ggcttggcct acctctttat
     3121 atcgcacgac ctggcggtgg tccgcagcat cgccgaccgg atcctggtga tgcgcgaagg
     3181 tcgtattctc gaatccgccc cgacggatcg gctttacgaa agtccgtcgc acgagtatac
     3241 ccggacgctt ttggcggcgg tgccggatcc gtttcggaag tgaatagtga atggtgagta
     3301 gtgactagtt ggaaaacccg aacaccctcc atgactcccg gcacgttcga aattctccag
     3361 caggcgcccg gcgccggagc gcggttgggc cggctctgga cggcgcacgg gccggtggat
     3421 acgcccgttt tcatgccggt cggcacgcag gccaccgtca aggccatgac gccggcggag
     3481 atggaggatc tgggctttca gattgtgctg ggcaacacct accacctgaa catacggccg
     3541 ggcgtggaca tcatcgagga gcagggcggg ctccaccggt tcatggggtg ggagcgggcg
     3601 atcctgacgg acagcggcgg gtaccaggtc ttcagcctcg ccaggctcaa caggatcacg
     3661 gccgacggcg tcatcttcca gtcgcacgtc gacggcgcca cccacgtgct cggccccgag
     3721 tccgccatgg cgatccaacg gcggctgggc tcggatattg cgatggtttt cgacgagtgc
     3781 gcgccgtacc cgtgcgacag gaaatacgct tgccaagcgg tggagcggac cctagcatgg
     3841 gcggccttat gtgcgcggca gccgagggca gccgggcaac tggtctttgg cattgtgcag
     3901 ggcagcgtgt atgcggacct ccgcgagcgg tgcgcccgcg ggctgatcga actgggcttc
     3961 gacggctacg cgatcggcgg cgtgagcgtg ggggagccgc acgcgctgat cctgcagggg
     4021 atcgaggaca gcgtgcggca cctgccggcg gaccggccgc gctacctgat gggcgtcggc
     4081 tcgcaccagc acatggtcga ggccgtcgcg cggggcgtgg acatgttcga ttgcgtcatg
     4141 ccgacccggc tggcgcggca cggcacggcg ttcacgcggg cggggaagta tcccgtcaag
     4201 gcggccctgt acgcccgcga tccccggccc atcgaggaag gctgcggctg ctatgcctgc
     4261 cgccatttct cgcgggccta cgttcggcac ttgatgaacg tcggcgagat cctggggatc
     4321 cgcctgttgt ccattcataa cctgttccgg tacgaggcct tcgtgcagga gatgcgcggg
     4381 gcgatccggg ccggcgggtt cgcggagtac gcgaaaggat tcgtggaaca gtatggaaat
     4441 ggccgggacc aacccggccg cgacgagtaa ggagcgggac gaatgaattt tctgatgacg
     4501 tggttggcca tggcgcccgc gcccggcggg acgggcgggc agcagcagtc cccggcgttt
     4561 atgatcgggt ggctcggcct gatgctggtg attttctact tcatgctcat ccgcccccag
     4621 cagcggcggg aaaaggaacg ccgcgccctg ctcacgcaga tcaagagcgg cgaccgcgtc
     4681 gtgttctgcg gcgggctcct gggcattgtc acgaacgcca aggaaaagac cttcaccatc
     4741 aaggtggatg agggcgtgaa actggaagtc gtccgcagtg ccgtaacgca agtgctggaa
     4801 aagggcgaga cgcccgagga tagtccggcc aagaagtgac gtacgtctca actcgtggtc
     4861 tcgaaagagg attcggaaac atggacaagc attcgctctg gaaatggttg gtgctcatcg
     4921 gcttggtggc ctggtcaatg gtgctggtca tgccgctgca tgaaaaagtg aaactcggcc
     4981 tcgacctgcg gggcggcatg agcttcgtgc tggaggtgga caccaccacc ctggcgccgg
     5041 acgccatctc agacgcccag gcgcgcgccc tggaagtcat ccgcggccgc gtggacgcca
     5101 tgggcgtggc ggagccgatc atctatcccg aacccggcaa caaccgcatc gtcgtccaga
     5161 tgcccggcct cggggccgag gaccgcgacc gcgcggtccg caatatccag agcgccgcct
     5221 tcctcgaatt ccggatggtc catccgaaga acgacgacct ggtcgaggac ctgttcgatc
     5281 agaaactggt ccccgacggc tacaagatcg tgtcgatcga cgagcagatg cccagcggcc
     5341 agtggaaagc cgtcaattat tacaagcgcg atgtgtctct cgacacgccc ggcacgacgg
     5401 atcgcgaggt gcgcgaccgg ctgaaactct tcaagcccca gtcgaactac gatttcatgc
     5461 tgatgaagga aatccggaat gggcaggagt tgtttcagcc ctattacgtc cggcgcctgg
     5521 ccgagttgaa gggcgaggac ctcatccgcg ccggcgtgga tttccgaccg ccgctgaacc
     5581 agcccatcgt caccctgaag ttcgacgcca agggctcgcg acggtttgcc aagttgactt
     5641 ccgattacgc ccccggcggc caattgaatc cgggcatgga cagccggcgc tatctcgcca
     5701 tcgttttcga cggccaactc cgctccgccc cgttcatcaa gaccgccatt cacggcggcg
     5761 aagccatcat cgagggctcg ttctccgtca aggaagcgca ggacctgtcc atcgtcctgc
     5821 gcgccggcgc gctgcccgcc cccgtcaagg tcatggagga gcggcgcgtc gatccgtcgc
     5881 tgggaagcga ctccatcaac agcggcaagc gcgcggcgat ctatggcggc atcggcgtgg
     5941 tggtgttcat gtgcatctac tacctgggcg ccgggctggt ggccaacctg gccctcgtcc
     6001 tcaacctggt gctcctgccg ttcggcatgt ggctcgtggc gggcttcttc agcatcctgg
     6061 aaaccagggg cctgggcggg ggcgggatga tcagcctgcc gaccctcacg ctgcccggcc
     6121 tcgccggtat cgtgctgacc gcgggcatgg ccgtggacgc gaacgtgctg atctttgagc
     6181 gcatccgcga ggagcaggcg gccggcaagc gattcgccgc ggccatcccg gccggctatg
     6241 acaaggcctt cagcgccatt ctggactcca acctgaccac gctgctggtg gccatcatcc
     6301 tgttctggca gggttcgggc ccggtccggg ggtacgcggt caccttgagc gcgggcatcg
     6361 tggtcagcat gtacacggcc atcaccgtca cccgcatgat cttcaccacg ctggcccagc
     6421 ggttcgacat cggctcgatg aagatgctcc agttcttcaa gtccacgaag atcaacttcc
     6481 tgcgctggcg ctttgtagcg ggcgggcttt cgctgaccat catcatcgtg acctgggtgc
     6541 tgtttgtcct acgcggccag gacaactttg gcgtggattt caccggcggc tcatcaatca
     6601 cgttccagtt cgaggagaag cggcccgagg aaaccatccg gcagctcctg gaatcggaag
     6661 gcatcgagga tccgttcatc cagtaccagc ggctggtcac cgcggagagc ggcggcacgg
     6721 aggtcgagta cctggaagtc aaaaccccct tcgccgacgg cgacaagacc gcatcggtca
     6781 taacggagaa actcgcggac gcgaaatact ccgtgctgaa gcaggacagc atcggcccgc
     6841 aaatcgggca ggagctcaag cgcaagggtc tcaaggcgct gatcctgtct ttcatcgtga
     6901 tgatcatcta cgtctcctgg cgttttgaat tcgggtacgc gatcggagcg ctgctggccc
     6961 tggtgcacga cgtgctggtc accgtgggca tctataccct gctggggcgc cagcttagcg
     7021 tgacgaccct ggccgccgtg ctgacgatcg tcgggtattc ggtcaacgac acgatcgtca
     7081 tcttcgaccg tatccgcgag cagttgaagc tcaaccgcgg catgaagtat gccgacgtcg
     7141 ccaacatgtc catcaaccag acgttgagcc gcacggtgct gacctccggc acgacgatga
     7201 tcacggtcct cgccctgctg ctgctgggag gcggcgcgat caatgatttt gccctggtca
     7261 tgttcatcgg tatgattgcc ggcacctact cgacgatcta catggccacg ccgatcgccc
     7321 ttctgtggca tcgcgagaag ccgcgcgagg acgagaagaa ggcctaaggg tagggcggct
     7381 tggctcaagc cgccgcgtat cggcgcgttg ggccaacgcg ccctgccggt ctgcgttatg
     7441 cccaagctgt ggaaaaccgt cgagcgggtt ggccgggagg ccgatccgct cgcggcgtct
     7501 ctgggcatcc cccggccgct ggccgaggtc ctggccaccc gcggacttgc cgagccggtg
     7561 gaggcacgcc ggttcctcaa cccccgtctg gatgatctgt ccgatccgac cgttttgccc
     7621 gatatgcccc gggccgtgga ccgcatacgg cgggccgcaa aggggcgcga gcgcatcgcc
     7681 gtgttcggcg actatgatgc cgatggcgtc acggccgccg ccctgttgac ccgttgcctt
     7741 cgggaactcg gcgccgaggc ggagccgttt ctgccgctgc gtctggagga gggctatggc
     7801 ctgggcgtgg acgccctgaa tcgctgtctc gaggcgcacc gcccgcagct cgtgatcacc
     7861 gtggactgcg gaacgggctc cgtggacgcc gtgcgcgccg ccgcggcggc cggggtggac
     7921 gtggtcgtga cggatcacca tgaacccgcg ggcgaaatcg cgccggccat cgccgtggtg
     7981 aatcccaaac tcggcgacgg tggggacctg gccaacctcg cgggcgtcgg cgtggccttc
     8041 aagctggcct gggccctggc cctccgctcg ggcgtggaca tccggcggat cttccgccgc
     8101 ctcgacctgg tggcgacggg gaccgtggcc gacgtggtgc ccctcacggg ggagaaccgg
     8161 attctcgtcc actacgggct cgggcagctg aaccgcgagc cgctgcccgg cctggcggcg
     8221 ctggccgagg cggcggaagt gaaagacgcc atcggggaat atcacctggg cttcgcgatc
     8281 ggtccccgtc tcaacgcggc cgggcggctg ggcgatgcgc aggccgcgct ggaactcctg
     8341 ctgggcgacg acccggaggc cctcctcccg ctggcgcggc agttggaccg gggcaaccgc
     8401 gagcggcaga agatcgaggc ggatattctc gaagaggcaa tgcaggacgt agccgcgctg
     8461 gatctggaga ataacgccgg attggttctg gcccgccgcg gctggcatcc gggcgtgatc
     8521 ggcatcgtgg cgtcccggat agtccagcga ttcgggcggc cggtcctgat gatttcgttg
     8581 aacgaagacg gcacggggcg ggggtcgggc cgcagcatcg agggcttcaa cctggtggag
     8641 aacctggccg cctgcaggga ttcgctggtc cggttcggcg gtcacgccat ggccgccggg
     8701 ctcgagttga aggagaagaa cctggcggcg ttccgcgagg ctttcaaccg gcgtgtgtcg
     8761 gagaccgtgc cacctgacca gcttcggccc gtgcagcggg tcgacgcttg gctcgacctg
     8821 gcggacgccg acgagcggct gttcgagggc ctgcaggcgc tgcggccttt cggctgcggc
     8881 aatccgcgcc cggtctgggc atcgtcgcgc gtgcgcgtgc tgggccagcc gaggatcctt
     8941 aaggacaagc acctgaagat gcgcgtcgtg tcgggcagcg cccagcggga agcgatcggt
     9001 ttcaacctgg ccggccagcc gctgccggac gggccgatgg acatcgcctt cacgctggac
     9061 atgaattcct acatgggccg tgagacgctg cagctcaagc tggaagactt ccggcccagc
     9121 ccctgacccc gcgcccatcc gtaacaatac tattcacacg tgtgaagtaa aatgttgcac
     9181 tcgaaacgga ggggaatgag cgttggggcg ctgctggggc tgctccttct gcagccctgt
     9241 tttgccggga cgcgggagga gcgcctggcc gtggtccagt tcatccatcc gggctcggaa
     9301 cacgagccgg attcggcgga tggccgctcc tggaacacgg atgcgcaccg ccggaagttt
     9361 ctcctccagc cgggccgcta tctcgcgtcg ctggaatcca aacccgtcgc cgcggacctc
     9421 gtcttctggg gcgagttcga gccgccgacc cggctggtca ggacattccc cgagaccggc
     9481 ggggacatgc cccggcacct ttttacaccc gatccggtgg ccttctatcc gcacgatccg
     9541 cccttgatga acacggatcc cttcgtcttc ggcggccggt ttttctacag catctgcaag
     9601 cagaacaacc ggcgcggccc gaccgcgctt cagcgcctgg cccgcggttc ggtcattctc
     9661 ttcggctccg gccgcggacg cgaccggttc gtggtcgaca cggtcttcgt cgtcgccgac
     9721 tacgtggact ggaacctctc caactaccgc gagcggctga aggatgtcgt gccgccggaa
     9781 tacttccact cgaccctcga acccattgcc tacgagatgc aagtccggaa cctgacgccc
     9841 tcgcagacgt tccggctgta catcggcgcg acgttcgacc ggccgtgcga ggggatgtac
     9901 agtttctttc cctgccggcc gtatcggcag gaagccaata cgggattcgc gcggcccgtg
     9961 ctgcgccggc cggggatcat caccgacaac ctgacccagg gccagcggct caatccgatg
    10021 ccggacgcgg cgacggtccg cgaaatctgg gaggacatcg cgcggcaggt gctggggcag
    10081 gggctcgacc tcggcgtgca tgccgagatg ccgcggatca ggcaggtagg gcggcttggc
    10141 ccaagccgcc gtgatacggc gcgttgagcc aacgcgccct accggttcgg cgtgaacagg
    10201 ttccgcccct cgaatcgctt cgtaccgttg acgtaactgt agtacttccg gcactcgcag
    10261 caaaggtact cgacgtcgat gggcgtgaag gcccgccgcg cctcgggcca atagacggac
    10321 tgcagttcga tcatcttcgc catggtcgct tcgtgatccg gcagcccgag gtgtttctgc
    10381 agcaggtcca tgaacggcgc ggacccgatg ccggtcggga ccgggctgtc cggccggatg
    10441 agcgacggat cgaaccaggc gatatcggcg acggccatga agatgggaaa gtcgttgctc
    10501 atgcggaatt ccgcgttgat tcgccgcgtg gccgtgacca cgtggcccgc cgcgccgcgc
    10561 acgcaggcct ccaggaaccc gagcgcgcgc gggatgagtt cgatgccgcc ttccaggcga
    10621 tcgtacgtcc gattctccca gcgtgcttcg accacgggat aggcttccga gaaccacggc
    10681 tgggccgttt catgcaggag aaaatgccgc agccgcgccg gctgtcgaag gagagggggc
    10741 tccatcgcgt caagcgtcgt gtcccggttg caccagcggg caaagaacag ggcgtgcagg
    10801 agttccggca gggacgatcc cgcccgccgc acgaaccgga cgacggcccg ggagccccgg
    10861 tcgtcctcgc ggaagacgtt caggaaccgg ccgcgctgga agatcgggtc ggcgctccac
    10921 tgcggcggtt cgccgcgctc gcgcttttcg cggatgcgct cccgttcccg gcaccagtcg
    10981 aagaaagccc gcacgggatc agccagctcc aggccccgga gcgcatcccg gtagggattc
    11041 gccgcggagt tcacgcgctg caccatattt cgctgacccg gtccgggaag gtcaggctca
    11101 cgccgatgcg cgcatggccg gcctggtcga gcatctgccg ggccgtcttc agggccaggt
    11161 cctggcggtt gcactcctcg ctcaagtgaa cgaggaacac gtgctccaat tccgggccgg
    11221 cgagttcgca gaccaggcgc gccgccgagg cgttggagag gtggccctgc cggccggcga
    11281 tccgttgctt cagttgccac gggcgctcgg cctccaggag cagccgttcg tcgtggttcg
    11341 attcgattac cagcacgcgg cagcccttga gccgctcgcg gaccaggccc gtcgcaacgc
    11401 cgatgtcagt ggcgatgccc acccgctcgc ggcccgcggc gatgacgaag cccaccggct
    11461 cgtaggcgtc gtgggacacc gcgaacggct ccacggtcag gtcgccgatg gagaaggcaa
    11521 atcccgtgct gaataccttc cacgccaggt cgttcaggtc ggggttgcgc tggagcgcct
    11581 cgatcgtccc ggtgttggcg tacaggtcca ggccgtgccg ccgctgcaat gcgcgcagcc
    11641 cggccgtgtg atcgttgtgt tcgtggctga cgcacaccgc ccggatgcgg gcggccggta
    11701 tctcgagcag gtcgagacga cggagcgtct ccttgccgct caacccggcg tcgatgagga
    11761 tccccgtccg cgtggatccc acgaaaatgc agttccccga actgccgctg gccagcacgc
    11821 aaacctgtaa agacatggtc ttccgattct ccggccgcga gtataccata ttgaagccgc
    11881 gggagaatcc tgatgactcg gcttgatttt caggccgcgg acaggcattt tctccctgcc
    11941 gacaggcaaa aagacgggag aaaccatgag caacgcgcag acatttccga cgatcctggt
    12001 cacgggcggc ggcggttacg tgggcagcgc ccttgtgccg gccctcctga agcaggggca
    12061 caaggtgagg gtcgtggaca cgttctggta cggcgagaac gtgttcgccg actgtaacag
    12121 ccacccggcc ctgacgcgca tcgcgaagga catccgcgac acggccgcca tgaaggaggc
    12181 catgcgcggc gtggacgccg tcatccatct ggcctgcatc tccaacgatc ccagcttcga
    12241 gctcgatccc aagctcggca agggtattaa cctggacgcc ttccccggcc ttgtcgaggc
    12301 cgcgaaggcc ggcggcgtga agcggttcat ctacgccagt tcgtccagcg tgtacggcgt
    12361 caaggagcag cccgacgtcc gcgaggacga tccgcctgct ccgctgacgg actactccag
    12421 gttcaagctc gagtgcgaga aactgctgat gaaactcggc ggcgggatga cccgcgtgat
    12481 cgtccggccg gcgacggtct gcggctacgc gccgcgctta cgcctcgacc tgacggtcaa
    12541 catcctgacg atccacgccc tggtgaatcg caagatccgg atcttcggcg gcaggcagat
    12601 gcggccgaac ctgcacgtgc tggacatggt ccgcgcctac gagatgttcc tcgcggcccc
    12661 cgcggagaag atcgacggcg tgccgttcaa cgtcgggttc cggaacgaga ccgtcgaaaa
    12721 catcgccggg atcgtcaagc gcaccatcgg cgacgagggc atcgcgctgg aatacgtgcc
    12781 gaccgacgac aaccgctcct accatgtcaa ttcggaccgg gtccgccggg tcctgggctt
    12841 cgaaccgaag tacaacatcg aggacgccgt gcggagcatc gtcgaggcct accgcgccgg
    12901 gaagatcccg gacccgatga ccaacccgat gtaccacaac ataaagcgga tgcaccagtt
    12961 gtcgctgggc tgaaggcgga tcgcgtgcac ctcggcgtgg atttcgacaa cactctggtc
    13021 agctacgacg aactgttcca ccggtgcgcg ctggagcgcg gcttgatccc cgcggacctg
    13081 ccgcgaacca agtcggccgt ccgcgcgcat ctctgggcgt tgccggacgg caacacgcca
    13141 tggaccgagt tgcagggatt ggtctacggg acgcgcatgg cggaggcggc cttttttgcc
    13201 ggtgcgcgcg aggcactggt cctgtgtcgg gagcgcggca tccgggtttc catcatcagc
    13261 cacaagctgg agtttccggc gcggggcccg cgcgtaagcc tgcgtaaggc ctcgctggaa
    13321 tggatggagg cgcaggggtt cttcgatccc gccgcgatag gattggcccg ggaggcgata
    13381 ttcttcgaat cgtctcgaga ggcaaagctg gcccgcatcg cgggcgagcg ctgcacccat
    13441 ttcgtggatg acctgcccga ggtgctggac gcgcccggtt tcccggctgc cgtcgagaag
    13501 tggttcttcg atccgaccgg cagcgccaaa tggcccgcgg gcgcccggcg attcggctca
    13561 tgggcggaga tccggagcca cctggagacg atgagaccat gataccggac attccgaagg
    13621 aaatcctgtc cgccctcgag cggatgctgg gccgcgcgcc ggagcgggtc acgcggcttc
    13681 cgggcggcgc gaacaatctg gtcctggaaa tcgtgctggg gcattcccgc tacctggcca
    13741 aggcatattt tcagcacaag aacgatcccc gcgaccggct gggcgcggag ttcggcatgc
    13801 tgacgttcct ttggcggcac ggcgtcaggg aggtgcccga gccgctgcag tccgacccgg
    13861 cgcggaagat cgggatcttt gaatacgtgg acggcatgcg gctcgcgccg ggcgaagtga
    13921 ccgcgccgga tgtccggcaa ttggtggacc tgctgaccgc catgtggcgg ctgcgcggcg
    13981 agtcggacgc gcaggacatg gcccccgcgt ccgacgcctg cttctcgatc cggggttaca
    14041 tggaaaacgt gggcggccgg ttccaacgca tccggaatgc gctggagcag ggcggggccg
    14101 tcctcgaggc cgaggcgtac atccgcggcg acatggcgcg gacatgggag cgtatcctgg
    14161 acttcatgga gcagggcgcg aaggaggcgg gcctggacgc ggccctgccg ctggagcgcg
    14221 cggaacagac gctgaatccg gccgaccacg gtttccacaa cacgctccgc gaggcgaacg
    14281 gccggctgcg tttcctcgat ttcgagtact ccggttggga cgacccggcg cagatgatct
    14341 gcaacgcgct gtggcagccc gccgtgcccc tgccggaccg gcagcggaag ccgttcctga
    14401 aacgggtatt caagggcctc gaaggcgacg cggccctggc cgcgcggtcg cggctggcct
    14461 acccgatcct gggcttcaag tgggcctgca tcatgctgaa cgaattcctc ccggtgtccg
    14521 aggagcggcg gcgtttcgcg ggcacgcggc cggaggaccg gcgtcgggag cagttggaga
    14581 aatcgcggcg gcggctcatg gacatcgagg actacctggc cgcgccgtct ctttttgatg
    14641 gaattgcctt ctgatcccgg tatagtgtct cggcctaaca cggatgatga atgagcgcag
    14701 ccttggacca gcgagccctc gaacagaggc tggaaatact caagatactt gagttctccc
    14761 ggcgtgggca catcgggtcc accttctcgc tgctggaaat cgtgcgcgtc ctctacgacg
    14821 acatccttcg gatcgatccg aagaaccccc tgtggccgga ccgcgaccgc ttcgtgttga
    14881 gcaagggcca cggcagcctt tcgctgtatg tgaacctggc cgagaagggc ttcttcccga
    14941 aagacgagct gttccgcttc tgcaagttca acggcatcct cggcgggcac ccggaatacg
    15001 gggtcgtgcc cggcgtcgag gcgagcacgg gcgcgcttgg ccacgggctc tccatcgccg
    15061 tgggcatggc gctggccgcg cgcatcgacc gcaagggcta caaggtgttt gcgctgatgg
    15121 gcgatggcga gtgcgacgag ggcagcgtct gggaagccgc cttgagcgcc ggcaagcaca
    15181 agttgagcca cctgaccgcg atcgtggact acaacaagat gcagtcctac ggcccgatcg
    15241 acgagatcca gtccctcgag cccttcgccg acaagtggcg cgcgttcggg ttcgccgcga
    15301 cggaggtgga cgggcacgac gtggacgccc tgcgcgcgct cttcaagagc ctgccgctgg
    15361 acccgggcaa gccgaccgcc atcatcgccc acacagtcaa gggcaagggc atgtcctgca
    15421 tcgagggcaa tccctcgtgg caccacaaga ccaagatcac cgacgccgag ttcgaccagt
    15481 tgtacgccga cctgggattc ccgcgaaaat gaggaaggtc tgtttaaaga gcatggcgaa
    15541 gctggccgcg atggatgagc gcgtggtctt catcggctcg gacatcacca agcgcgacct
    15601 tgaggacttc tcggacaact tccccagccg cttcttcatg gagggtgtct acgaggcgca
    15661 catcgtcggc atggccgccg gcatggcgct gtgcgggaag atgccctaca tcaataccat
    15721 cgccacgttc ctgacgcgcc ggtgctacga gcaggtcctg ctggacgccg gcctgcacaa
    15781 gctccccgtg cgcctgctgg gcagcggagg cggggtggtc tacgcgccgc tcgggccgac
    15841 gcacctcgcc aacgaggaca tcgccatcct gcgcgcgatc ccgcacatgt gcatcgtcgc
    15901 gccgtgcgac gccgaggaaa tggcgcgcct gatgccgctc accctgaagt ggcccggccc
    15961 catgtacatc cgcttcgcca agggcggtga caaggtcgtg tcgcggcccg atctgccctt
    16021 cgagatcggc aagggcatcc tgatgaagga cggctccgac gttttgctgg tcacgaccgg
    16081 catcacgaca cagatcgccc tcgaagccgc cgcgacgctg gcgggcgagg ggatcggcgc
    16141 ggccgtcctg cacatgcaca cggtcaagcc gctcgacgcg gagcaactga tcgccctcgc
    16201 gggccgggcc cgcgcggtag tgacggccga ggagcacacg atcatcggag gcctgggcag
    16261 cgccgtggcg gagaccttgc tcgaggccag cgtggcgaag ccgttcaagc gccttgggtt
    16321 cccggatgtc ttcaccgagg aactcggctc ccagaacgac atcatgggca agtacggaat
    16381 cagcgtggac ggcctctgca gcacggcaaa aacgcttttg aagatcagct gaccggaatc
    16441 cagcaaccag aggagatccc atgaagaagc agatagactg gctccaggaa tacaacatgt
    16501 cgagcaa
//
LOCUS       DAOOTK010000065        16451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2413_length_16451_cov_5.862832, whole genome shotgun sequence.
ACCESSION   DAOOTK010000065 DAOOTK010000000
VERSION     DAOOTK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16451
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_16451_cov_5.862832"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2121)
                     /locus_tag="PK634_08525"
     CDS             complement(<1..2121)
                     /locus_tag="PK634_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOW97818.1"
                     /translation="MNRKQLAGAACLALALSVASSRAVRVRVATWNVQDGVGTPGEAD
                     YASVVATLQRINPDIIAFQELNNGDFNDWISVAAALGYPYYAMGDGGTFDAGKHNGFF
                     SRFPILSTEQVREPAGAAEINRWPLKAVIEIPGALNPLTLYSLHNKSASGETEDFMRA
                     IELRRTIAHVSASRQADPIPAEYVVMGDFNADVGVSQREQIPALPGGLPAGYVLGADV
                     TFPVAYRLHPTDPFAGIQFSQLEPFQEDSILQYTHLAPVNPRRYDYICFSEEVAASPY
                     GAPAGEIYYSIQDDGQGGLLKFSVPPPAETSANASDHLPVYADINMIDLLPCYNPVVL
                     ISEVASAADPRANFVELYNSGVAPFPLAGYRLVIHRNGAEALSVDLNGSIPAGSAFVL
                     AADAAAFAAAYGVAAGQASAQLLPINGNDPVTLLNPAGAVQDAYGAPGQAVAENDFSM
                     PWAYPQRAATRVLGTCDPLPQWRADEWSLLLSPTPGAHTACDQAGVFVDAMALQPAAP
                     RFNEEFRIRAAAHRNLLADGLAAVALYRWPGQSWTEAPMSLLQGELWESAAITLPPGD
                     ASSLEYAVRVYFNGPNAASPLLSAVRTYVFPPFVGPQRPLISEVQANDAGGDDAQFVE
                     LVAPAGLNLRGYALTHHDGNDSGDNDLWPYVFGDVIVPNDGLADIEGRALGFVVVGQA
                     PVANVDLLLPGDLQNGPDGLVLTAP"
     gene            2310..2993
                     /locus_tag="PK634_08530"
     CDS             2310..2993
                     /locus_tag="PK634_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HOW97819.1"
                     /translation="MKTRDHTGRIAPRRAGIATLLLIAALAGCVRPAAGPCPPEWWAP
                     VPRESAASWEILPQDAGPGEVILSKRTELGILSNFAPTPFTLDGETYASVEGFWQMLK
                     YPEGPGDERLRDPSVAWPHTREEVGRMSAFEAKAAGTAANGNMKKLGIRWVTYRGQRL
                     EYSEKRQGEFYRLIRAAMQAKLEQNPEVRAVLLKTGGLILRPDHHQRPDDSPAMKYYD
                     IWMELRDTL"
     gene            3022..3783
                     /locus_tag="PK634_08535"
     CDS             3022..3783
                     /locus_tag="PK634_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOW97820.1"
                     /translation="MDLNLKQEFAERWARYFPGAELPVAFFYASDPGSVREAAAAAGH
                     RCFIGDLAAVRRGTPLAFRAEALTCGGAQRYLGFTQKLRPNFEYFLSCGIPGQLEGER
                     YKQSPALVTEALKRQPPFQAPGRWLVAKRWDVLEAGDEPFAAVFFAPPDVLAGLFTLA
                     NFDETDPDGVRAPFGAGCASIVYWPLQELRAERPRAILGMFDVSARPCVGPNRLTFAV
                     PWPKLVRMARNMEESFLRTESWKKIMGRIRTASAD"
     gene            3902..4726
                     /locus_tag="PK634_08540"
     CDS             3902..4726
                     /locus_tag="PK634_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97821.1"
                     /translation="MTTSDGARDDTTERVLEQAVARETAVHGRRWAAQYGGYFNDPAV
                     AAPFLDVIEDGLRAKAPDVLVDLGGGTGFILAELIRRGKLSPAVKLYDMDLSDRQLES
                     LADARIRPLKSSFLEFRRDAVAAGAERLMLITRSTLHYAGLAGQKPLLRHLRGQLRPG
                     ELFIHQTACNARPEDALLVDELLERLHSEKWLPPLDSLVRLCEDAGFRVARVADAPDL
                     AMDEATLADRYGIAPGEMADIRARLGEAYADSPRWRSTPEGFVALLGYKILVCEAA"
     gene            complement(4740..5615)
                     /locus_tag="PK634_08545"
     CDS             complement(4740..5615)
                     /locus_tag="PK634_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97822.1"
                     /translation="MNRHPEIGRMAGAVLGLLVAGAPAWCGAGMRPFQPATKRVTVPV
                     QYPSLPPPQNVAAALLPVDLPLDGLVRYFPRGGIDCDGQRFSWWARMGGRVVRNDAAY
                     FPGFWQTNDRIIAMLQSIGVGTAPVCSGEDIWARAAAVWVWLRDHARPGPSYGYGSPD
                     RWPSLDEMAAMYERFGTVPWYACFSRAHLFATLLGSVGIPADHVAIASAHYPYDNENG
                     YAQHTYVALRVDRRWYYLDPSYVNTLAELPPYGQRASYGLRAGCQYSYPYEAVVLPGS
                     SLDTVPMLYAAPPAP"
     gene            complement(5672..7651)
                     /locus_tag="PK634_08550"
     CDS             complement(5672..7651)
                     /locus_tag="PK634_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOW97823.1"
                     /translation="MNHEHPLPPPGRACAETQFTDLFSRFGVSLDVQAAGPGLSRGHL
                     ASQREGKTFAAPLLAVSLTGENGEPLRRGACGQQWFPWGVVQECAWGVALRLRLTVVY
                     AAPDVAALHAEIANAGPRDEIFMVALESRLAPGDVNAAARFDATTNTAWLEAVSRPTS
                     RWTIRDEPDFRLAAGIRPLFAVASAEAAAGLCTVRSALLAVGRGDKASLAVLVSAAAR
                     DLSVPDAAAPRALVEARLEQQARRGLLEILAEARQRWRAVEARLPLADLEEECRDLAR
                     LSAVILLKNTLRPQPEQGYGGAMGGHLGTFPARGFYEAFWIWDSAKQARGFLEFDLEL
                     ARENILVMLDRQDEATGQLVFLQPDASVPSAQPPLFSWVTQELARKDPAAAPGFLAQV
                     YPRLAKWHRWWQDTRDRDGDGLAEWPDNLASGWDDSPRWDTGRQAGVGNDGGAPAYAA
                     VDLNAFLIADARHLAAMAGPLGRVAEARNWNARAEALSARVLAKLYDAEDNLFYDARG
                     DTGLFNRVKTTACFMPLWAGVPLAPEKIRDMLERYLLNERHFFGRFPFPCVAYSEPRF
                     DPAGASGYWRGPVWLDQAFFMLSILDRYRRLLGPASRDSADLARRRILDMVLRAGIHE
                     NYHALTGAPGANSREHFSWSAAAVLAIALRRYEES"
     gene            complement(7660..8463)
                     /locus_tag="PK634_08555"
     CDS             complement(7660..8463)
                     /locus_tag="PK634_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW97824.1"
                     /translation="MKPSRVYAIVLRQFWLIRGSFARVFPLFAWAAIDIILWGFISRY
                     LGVFTAAGIDFVPIFLGAVLFWDFFIRVMQGVTMAFFEDVWSRNFLNLFATPLSVSEY
                     VTGLVLSSVGTSAIGLAVMILLATAAFGLPFAAYGLMAVPFLLILFVFGVSLGIFACA
                     MVLRLGPAAEWFVWPIPALISPFAGVFYPIATLPAWMQGVAYALPPAYVFEGLRAVVA
                     GGTVPAGALARGGILAAAGLLLACGFFARTYRHTVRTGLLARYTAESVS"
     gene            complement(8460..9227)
                     /locus_tag="PK634_08560"
     CDS             complement(8460..9227)
                     /locus_tag="PK634_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW97825.1"
                     /translation="MSGLAAAPVLSVSGLRKRYGDTPAVDGVSFAVGRREIVGLLGPN
                     GAGKTTTINMILGVLEPSAGSIRVAGLDVATRRSKALERTSFAAVYAPLPGNLSLERN
                     LRIFGLLYGVPDVRARVDDAIRRLDLDALRGRKCGVLSSGEQTRASLAKALVSDPDLL
                     LLDEPTASLDPATARDIRVKIREFAAERGCGVLWTSHNMFEVQEICDRVLFLSHGRIL
                     LEGDPRALPAQHGKASLEELFIAVAREPLSLEQGAAP"
     gene            complement(9224..9373)
                     /locus_tag="PK634_08565"
     CDS             complement(9224..9373)
                     /locus_tag="PK634_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97826.1"
                     /translation="MTPAGWIIMILACGGFIGLLVWCIHKVVATPEAAEHLHAQTDID
                     TGDRE"
     gene            complement(9370..10944)
                     /locus_tag="PK634_08570"
     CDS             complement(9370..10944)
                     /locus_tag="PK634_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOW97827.1"
                     /translation="MSKREYWGTRVGAILAVTGSAVGLGNFLRFPGQAAKYGGGAFLI
                     PYLVAFLVVGLPMAMAEWALGRWGGRRGHNSAPGIFRMLLGSRKGAYVGMIGTIMPLM
                     IYMYYLFVEAWCLSYAWDYLTGHFGRLGADLAVIEDHFLTLVGARANGALFTPAAARM
                     LLFLGLCFVANTFLIYRGITRGIEKFCNLAMPLLVVCALVVLGRVLTLPANPAHPEQS
                     VVNGLGYMWNPTKPGETLWSALANPEIWLAATGQIFFSLSVGFGLILTYATYLKPNDD
                     VALSSVTACAGNEFCEVVLGGMIAIPAAFVFLGPAVVQNPPGTFGLGFVTLPNVFNQM
                     PLGSFFGFLFFFLLFLAAITSSLSMLQPSISLLEEGLKIGRRASVSILTFVTLAGCFF
                     VVYFSKDFTALDTLDFWMANFFIFIFATIQSVVFAWVLGIGKGMEEIRRGAEIRLPRA
                     LPFVLKYVSPVYLLAIFALWAWKNLPERWQAIVTLPKEGPPVVLLSVVLIAAVVAFFS
                     MIVAAANRAWDKEEPS"
     gene            complement(11060..12739)
                     /locus_tag="PK634_08575"
     CDS             complement(11060..12739)
                     /locus_tag="PK634_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOW97828.1"
                     /translation="MKNESIPEPSEAAAPAKHFIRQIIEDDLRAGKNGGRVVTRFPPE
                     PNGYLHIGHAKAICIDFGMALEHGGECHLRMDDTNPTKEEVEYIESIQEDVRWLGFDW
                     GKHFYFASDYFGRMFDCCAELIRKGKAYVCGLTQDEWSKTYRGAPPAAGQPSPYRDRP
                     AEESLRLFEEMRAGKHADGALCVRAKIDMASPNLHLRDPVLYRILHAGHPHTGNTWCV
                     YPTYDFAHPLEDAFEGITHSLCTLEFEVHRPLYDWVIDNLDNLPSRPRQYEFARLNLT
                     YTVMSKRRLLELVNEGRVRGWDDPRMPTIGGLRRRGYTPEAIRAFVEAVGVTKFESLS
                     DVALLEHCVRDDLNKRAARRLAVLNPRRVVIENASEIPAEVDAVNNPEDKSAGTRKVP
                     FSSELFVEADDFMENPPPKYFRLTPGGSVRIRYAGFLTCLRVEGDAVVCRWSPPEAAL
                     KVKGTIHWVSAKHAVPAEVRLYDRLFSVEDPMAKAAAEGKDYHEFLNPDSLKTATGFV
                     EPGLAAANPLERFQFERLGYFCVDPDSKAGRLVFNRAVTLKDTWAKVAAKA"
     gene            complement(12890..15511)
                     /locus_tag="PK634_08580"
     CDS             complement(12890..15511)
                     /locus_tag="PK634_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOW97829.1"
                     /translation="MFRYARQVFWMLMALQPAGARGREGVPLDGGLPTAGQVRIRDSS
                     AAGLTLAVELAGFVRGSAVLPDGRAYDLALDPGGASGPIGQPDVPAFAQWILVPNGCE
                     VRVEAAPGAPEIFTGVQLAPVQPPPSDSGGSAPMARDEAVYARDADYPGVFASFEAVS
                     EIRGQAVRLVRLHPYQYNPRSGVLTVYPRLAVRLEFVGTPERRDPRLASRAFDGLLRG
                     APNAAEILDAAPAPRRGSAAFGGSTNGCELLIICADAFTNAAYQLAEWKMRRGIATTV
                     VTTNETGSATNQVRDYILNAYTTWSPAPAYVLLIGDSDFIPPWYKTDHPSGERKNSTD
                     LFYADMSASLDWIPDLAIGRWPVETAAQATNWAARVIAYESAPPDRSRFGRYYTNATL
                     CASFQGYTGTAYYGYSERRFVKSTEDLQNFLADAGYAAERIYAEYQGTNPTHWANWAT
                     SYSALFENDSPPLGGVALPAELLKPAFPWSGARTNIRLAVNSGTFLLTHRDHGNRSGW
                     SKPVFTKATDVDGLTNGVLRPVVWSINCQTGWFDNETDDSGAGTSASDECFAEHWVRH
                     ASGGAIGVLAPTRTSYSYYNDRLYWGMADAVWTNFLEYHGVSYTAAGAPAYRLADILN
                     RAKTYFLTKYSLSSSYTKCTMEIFHCLGDPTLEMWTGLPQDLTVAHTGRVAAGAAAVT
                     VAVAQAGALVCCVYSNRILGTAEASDGVATVALSPAPEGAGALWITVTAHNYLPYLGT
                     ATVFHADADADGLPDDWEEEYLGTTGSDPEADDDEDGLSNYGEWIGGFDPGDSNSVFS
                     AGAEPAAEPGAYVLQWPSAADRFYAIYRATNLPGPFLLLDGHIPATPPVNVYTDAPAA
                     ELDPLFYRLLVTTNAPP"
     gene            complement(15598..15873)
                     /locus_tag="PK634_08585"
     CDS             complement(15598..15873)
                     /locus_tag="PK634_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97830.1"
                     /translation="MARWLGWGALAYWAVLGLFLHKDWHYSWPMIGWALKMLCPLACL
                     ANLFGLVTALSLWNVRRHEAARLLFLHGPPMAAVAFAIWWLFFGLNI"
     gene            complement(15911..16279)
                     /locus_tag="PK634_08590"
     CDS             complement(15911..16279)
                     /locus_tag="PK634_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97831.1"
                     /translation="MDNLFSAMENFRSAQKAEEKAEFIAAKAEGMAQRLERLERIVAR
                     LVPLNRAIWDILSEAANLTEKELKDRLRQMASEQSAATRPGAPAPPCPKCGRPLERGR
                     DICMYCGIRHEVGSVFDLLQ"
BASE COUNT         2703 a         5686 c         5701 g         2361 t
ORIGIN      
        1 cggcgccgtg agcacgagcc cgtcggggcc gttctggagg tcgccgggca gcaacaagtc
       61 cacgttggcg accggcgcct gcccgacaac cacgaacccc agcgcgcggc cctcgatgtc
      121 cgccaggccg tcgttgggca cgatgacgtc gccgaacaca tacggccaga gatcgttgtc
      181 cccgctgtcg ttgccgtcgt gatgggtcag cgcgtagccg cggagattca gcccggcggg
      241 cgcgacaagc tcgacgaact gggcatcatc cccgccggcg tcgttggcct gcacctcgct
      301 gatcagcggg cgttgcggcc cgacgaacgg cggaaacacg taggtccgga cggccgaaag
      361 cagggggctg gcggcgttgg gaccgttgaa gtaaacccgc acggcatact ccaacgaact
      421 ggcgtcgccc ggcggcaggg tgatcgcggc gctttcccac aactcgccct gcagcaggct
      481 catcggggcc tcggtccagg attgcccggg ccatcggtac agggccacgg ccgcgaggcc
      541 gtcggccaac aggttccggt gcgcggccgc gcggatgcgg aattcctcgt tgaagcgcgg
      601 ggcggccggc tgaagcgcca tggcgtccac gaagacgccc gcctggtcgc aggccgtgtg
      661 ggcgccgggc gtgggagaga gcagcagcga ccactcgtcc gcccgccact gcggcagcgg
      721 gtcgcaggtt cccagcacgc gcgtcgccgc ccgctgggga tacgcccacg gcatgctgaa
      781 atcgttctcg gccaccgcct ggcccggcgc gccgtaggcg tcctgcacgg cgcccgcggg
      841 gttcagcagc gtcacgggat cgttgccgtt gatcggcagg agctgcgcgc tcgcctgccc
      901 cgccgccacg ccgtacgcgg ccgcgaacgc cgcggcgtcc gcggccagca cgaaggccga
      961 gcccgccggg atcgagccgt tcaagtccac ggacaacgcc tcggccccgt tgcggtggat
     1021 gaccagccgg taccccgcca gcgggaacgg cgcgacgccg gaattataga gctccacgaa
     1081 gttcgcgcgc gggtcggcgg cgctggccac ctcgctgatc agcacgacgg ggttgtagca
     1141 cggcagcagg tcgatcatgt tgatgtccgc gtacaccggc aggtggtccg aggcgttggc
     1201 gctggtctcc gccggcggcg gcacgctgaa cttgagcagg ccgccctgcc cgtcgtcctg
     1261 gatcgaatag tagatctcgc ccgcgggggc cccgtaggga ctggccgcca cttcttcgct
     1321 gaagcagatg tagtcgtagc ggcgggggtt gacgggggcg aggtgcgtgt actgcaggat
     1381 ggagtcctcc tggaaaggct cgagctggga gaactggatg ccggcgaagg gatcggtcgg
     1441 atgcagccgg tacgcgaccg ggaacgtgac atcggcgccg agcacgtacc ccgccggcag
     1501 cccgccgggc agggccggga tctgctcccg ctggctcacg ccgacgtcgg cgttgaagtc
     1561 gcccatgacc acgtactcgg ccgggatggg atcggcctgc cgcgacgcgg agacgtgcgc
     1621 gatcgtgcgg cgcagctcga tcgcgcgcat gaagtcctcc gtctcgccgg aggcggactt
     1681 gttgtggagg ctgtagaggg tcagcgggtt cagggcgccc gggatctcga tgacggcctt
     1741 cagcggccac cggttgatct ccgccgcgcc cgccggctcg cgcacctgct cggtggagag
     1801 gatggggaac cggctgaaga acccgttgtg cttgccggcg tcgaacgtcc cgccgtcgcc
     1861 catcgcgtaa tacggatagc ccagcgccgc cgccacggag atccagtcgt tgaaatcccc
     1921 gttgttcaac tcctggaagg cgatgatgtc ggggttgatg cgctgcaggg tcgcgaccac
     1981 cgaggcgtaa tcggcctcgc ccggcgtgcc gacgccgtcc tgcacgttcc acgtcgccac
     2041 ccggacccgg accgcccggg acgaggccac cgacaacgcc agcgccagac atgccgcgcc
     2101 cgccaactgt tttctgttca tggtggttcc tctttgacta tccaagtcgc ccgggccggc
     2161 ggtttcttcc cttgaagcgt ataagtctct tgcgtcggac cggtcagaaa aggcgcgatt
     2221 ccgcgagtcg gacccctgcc ggatccgccg aaccgcgggg gttttcatcc cggcctgttc
     2281 gcggcataat agggagaaag aggaccgcca tgaaaaccag ggaccatacc gggcggatcg
     2341 cgccgcgccg cgccgggatc gcgacgctcc tgctgatcgc cgcgctcgcc ggttgcgtcc
     2401 ggccggcggc cggaccctgc ccgccggagt ggtgggcgcc cgtgccccgg gaaagcgcgg
     2461 cctcgtggga aatcctcccg caggacgccg ggcccggcga ggtgatcctg tccaagcgga
     2521 cggaactggg catcctctcc aactttgcgc cgacgccctt cacactggac ggcgagacct
     2581 acgccagcgt cgagggattc tggcagatgc tcaagtatcc cgaagggccg ggcgacgagc
     2641 gcctgcgcga cccgtccgtc gcgtggccgc acacccgcga ggaggtcggc cggatgagcg
     2701 cgttcgaggc caaggccgcc ggcacggccg ccaacgggaa catgaagaaa ctcggcatcc
     2761 ggtgggtcac gtacaggggc cagcggctgg agtactcgga aaaacgccaa ggcgagttct
     2821 atcgcctgat ccgcgcggcc atgcaggcca agctggagca gaatcccgag gtccgggccg
     2881 tcctgctcaa gaccggtggg ctgatcctgc ggcccgacca ccaccagaga cccgacgact
     2941 ccccggccat gaagtattac gacatctgga tggagctccg cgacacgttg taatcaacat
     3001 cctgacgaaa ggaaccgcga catggacctg aacctgaaac aggaattcgc cgaacgctgg
     3061 gcgcgctatt tcccgggcgc ggagctgccc gtcgcctttt tctacgcgag cgatcccgga
     3121 tcggtccggg aggccgccgc ggcggccggc caccgctgct tcatcggcga cctggccgcc
     3181 gtgcgccgcg gcacgccgct ggccttcagg gcggaggccc tgacctgcgg cggggcgcag
     3241 cgctacctgg ggttcacgca gaaactgcgc ccgaacttcg agtacttcct ctcgtgcggc
     3301 atccccgggc aactcgaggg cgagcggtac aagcagtcgc ccgcgctggt caccgaggcc
     3361 ctgaagcgcc agccgccgtt ccaggcgccg gggcgctggc tcgtcgccaa gcggtgggac
     3421 gtgctcgagg ccggagacga gccgttcgcc gccgtgttct tcgcgccgcc ggacgtcctc
     3481 gccgggctct tcacgctggc caacttcgac gagacggacc ccgacggcgt gcgcgcgccc
     3541 ttcggcgccg gctgcgcgtc gatcgtgtac tggccgctcc aggaactccg ggcggagcgg
     3601 ccgcgggcca tcctggggat gttcgacgtg tccgcgcggc cgtgcgtagg gccgaaccgg
     3661 ctgaccttcg ccgtgccgtg gccgaaattg gtgcgcatgg cccggaacat ggaggagagc
     3721 ttcctgcgga cggaatcctg gaagaagatc atggggcgca tccgcaccgc gtcagcggac
     3781 tgatcggatg gcccggaatc tttcctggat gatgctatac cccggacccg gcgcgaggtg
     3841 aagaccggtt gcggcggacg gatcgcttca tgacggcccg gaaggaaacg aggaggcggc
     3901 gatgacaacc agcgacggcg cgcgcgacga cacgacggaa cgagtcctgg aacaggccgt
     3961 ggcccgcgag accgccgtgc acggccggcg ttgggcggcg cagtacggcg gctacttcaa
     4021 cgatccggcc gtggccgcgc cgttcctcga cgtcatcgag gacggcctgc gcgcgaaggc
     4081 cccggacgtc ctggtggacc tcggcggcgg cacggggttc atcctcgcgg agctgatccg
     4141 gcgcgggaaa ttgtcccccg ccgtgaagct gtacgacatg gacctgtcgg atcggcagct
     4201 cgaaagcctg gccgacgcgc gcatccggcc cctgaagagc tccttcctgg agttccggcg
     4261 ggacgcggtg gccgccgggg cggagcggct catgctgatc acgcggtcca cgctgcacta
     4321 cgccgggctc gcgggccaga agccgctgct ccggcacctc cgcgggcaac tgcggcccgg
     4381 cgaactcttc atccaccaga ccgcctgcaa cgcccgcccc gaggacgcgc tgctggtgga
     4441 cgaactgctg gagcggctcc attccgaaaa atggctgccg ccgctcgatt cgctggtccg
     4501 gctctgcgag gacgccgggt tccgcgtggc ccgcgtggcg gatgcgccgg acctcgcgat
     4561 ggacgaggcg acgctggccg accgctacgg gatcgcgccc ggcgaaatgg cggacatccg
     4621 cgcccggctc ggcgaggcct acgcggactc cccgcgctgg cgcagcacgc cggaggggtt
     4681 cgtcgccctg ctggggtaca agatcctggt gtgcgaggcc gcctgacggc cggcgccgct
     4741 cacggcgcgg gcggggcggc atacagcatg ggcacggtgt ccaggctcga gcccggcagg
     4801 acgaccgcct cgtagggata cgagtactgg cagcccgccc gcaagccgta ggacgcccgc
     4861 tgcccgtacg gcggcagctc cgcgagcgtg ttgacgtagc tcgggtcgag gtagtaccag
     4921 cgccggtcga cccgcagcgc cacgtacgtg tgctgcgcgt agccgttctc gttgtcgtag
     4981 ggatagtgcg cgctggcgat cgccacgtgg tccgcgggaa tgccgacgct gcccagcagc
     5041 gtggcgaaca ggtgggcccg ggagaaacag gcgtaccacg ggaccgtgcc gaagcgctcg
     5101 tacatcgccg ccatctcgtc cagcgacggc cagcggtccg gcgagccgta gccgtacgag
     5161 gggcccggcc gcgcgtgatc ccgcagccac acccagaccg cggccgcgcg cgcccagatg
     5221 tcctcgcccg aacagaccgg cgccgtgccg acgccgatgg actgaagcat ggcgatgatc
     5281 cggtcgttcg tctgccagaa ccccgggaaa tacgccgcat cgttgcggac gacgcggccg
     5341 cccatccgcg cccaccacga gaaccgctgc ccgtcgcaat cgatcccccc gcgggggaag
     5401 tagcggacga ggccgtcgag cgggaggtcc accggcagca gggctgccgc cacgttctgc
     5461 ggcggaggca acgagggata ctggaccggc acggtcaccc gcttggtcgc gggctggaag
     5521 ggccgcatgc ccgcgccgca ccaggcaggc gcgcccgcga ccaggagccc cagcaccgcc
     5581 cccgccatcc gaccgatctc gggatgtctg ttcatggaga aagccctcgg aaggctctgt
     5641 cgccgcgcgg cgttttttct tcccggggcc gtcaggattc ctcataccgg cggagcgcga
     5701 tggccagaac ggccgccgcg ctccaactga agtgctcgcg cgaattcgcg ccgggggcgc
     5761 cggtcagggc gtggtagttc tcgtggatcc ccgcgcgcag gaccatgtcc aagatccggc
     5821 gccgggcgag gtccgcgctg tcgcgagagg ccggaccgag gagccggcgg tatcggtcca
     5881 gaatggagag catgaagaac gcctggtcca gccataccgg cccgcgccag tagcccgagg
     5941 cacccgcagg atcgaagcgc ggctcggaat acgcgacgca cgggaacgga aaccggccga
     6001 agaaatgccg ctcgttcaac aggtagcgct ccaacatgtc ccgaatcttc tccggcgcaa
     6061 gcggcacccc cgcccagagc ggcatgaaac acgccgtggt cttcacccgg ttgaagagcc
     6121 ccgtatcgcc gcgggcatcg taaaaaaggt tgtcctccgc gtcgtacagt ttcgccagta
     6181 cgcgcgcgga caaggcctcc gcgcgggcgt tccagttccg ggcctcggcg acgcggccca
     6241 gtggcccggc catcgcggcc aggtgccggg cgtcggcgat gagaaacgcg ttgagatcca
     6301 cggccgcgta cgccggcgcg cccccgtcgt tcccgacgcc cgcctgccgg cccgtgtccc
     6361 accgcgggga atcgtcccac ccgctggcca ggttgtccgg ccactcggcc aatccgtccc
     6421 cgtcccggtc acgggtgtcc tgccaccagc gatgccactt cgcgaggcgc ggatagacct
     6481 gcgccaggaa gcccggcgcc gcggcgggat ccttccgcgc gagttcctgc gtcacccacg
     6541 agaacagcgg cggctgggcg ctgggcacgg aggcgtcggg ctgaaggaaa acgagttgcc
     6601 ccgtggcctc atcctgccgg tccagcatga cgaggatgtt ttcccgcgcg agttcgaggt
     6661 cgaattccag aaagccccgg gcctgcttgg cggagtccca gatccagaag gcctcgtaga
     6721 aaccgcgcgc gggaaacgtg ccgagatgtc cgcccatcgc gccgccataa ccctgctccg
     6781 gctgcggccg gagggtgttt ttcagcagga tcacggcgga aagccgggcc aggtcgcggc
     6841 attcctcttc gaggtccgcc agcggaagcc gggcctcgac cgcgcgccac cgttgccggg
     6901 cttcggccag gatttcgagc aggccccgcc gggcctgttg ctccagccgc gcctcgacca
     6961 gcgcgcgggg cgcggcggca tcgggcacgg acaggtcccg ggcggcggcc gagaccagca
     7021 cggccagcga ggccttgtcg ccccggccca ccgccaacag ggccgaccgc accgtgcaca
     7081 ggccggcggc ggcctccgcc gaggccacgg caaacagggg ccggatgccc gcggccaggc
     7141 ggaagtccgg ctcgtcccgg atcgtccagc gggacgtcgg ccgggatacc gcttcaagcc
     7201 aggccgtgtt cgtggtcgcg tcgaatcgcg ccgccgcatt gacgtcgccc ggcgccagcc
     7261 ggctctccag cgccaccatg aagatttcgt cccgcggccc ggcatttgcg atttccgcgt
     7321 gcagcgccgc cacatccggg gcggcataca cgaccgtcag tcgaagccgg agcgcgacgc
     7381 cccatgcgca ctcctgcacg acgccccagg ggaaccactg ctggccgcag gctccgcggc
     7441 gcaggggttc cccgttttca ccggtcaggc tcacggcgag caggggggcc gcgaaggtct
     7501 tgccctcccg ctggctggcg agatggccgc gactcaagcc gggcccggca gcctggacat
     7561 ccagcgatac gccgaaccgc gagaacaggt ccgtgaactg cgtctccgcg cacgcgcggc
     7621 cgggcggcgg aagcgggtgt tcgtgattca tggcattgtt cagcttacgc tctccgcggt
     7681 gtagcgcgca aggagcccgg tgcgcacggt atgccggtac gtccgcgcga aaaagccgca
     7741 cgccagcagc aggccggcgg ccgcgaggat cccgccccgc gccaacgcgc cggccgggac
     7801 cgtcccgccc gcgaccaccg cgcgcagccc ctcgaacacg tacgccggcg ggagggcgta
     7861 cgcaaccccc tgcatccacg ccggcagcgt ggcgatcgga taaaaaacgc ccgcaaaagg
     7921 ggagattagc gccgggatcg gccagacgaa ccactcggcg gccggcccga gccggagcac
     7981 catggcgcaa gcgaagatcc ccagcgaaac gccgaacacg aacaggatga gcaggaacgg
     8041 gacggccatc agcccgtacg ccgcgaaggg gagcccgaac gccgccgtcg ccaggaggat
     8101 catgaccgcc aggccgatcg cgcttgtgcc gacgctggag agcacgagcc cggtaacgta
     8161 ttccgagacg gacagcggcg tggcgaacag gttcaggaaa ttgcggctcc agacgtcctc
     8221 gaagaacgcc atggtcacgc cctgcatcac gcggatgaag aagtcccaga agagcacggc
     8281 cccgaggaag atcggcacga agtcgatccc ggcggcggtg aacacgccga ggtagcggct
     8341 gatgaagccc cacaggatga tgtcgatcgc cgcccacgcg aacaggggga agacccgagc
     8401 gaagctgccc cggatgagcc agaactggcg cagcacgatg gcgtagacgc gcgacggctt
     8461 cacggggccg ccccctgctc cagcgagagc ggttcgcgcg cgacggcgat gaacagctcc
     8521 tcgaggctgg ccttgccgtg ctgggcggga agggccctcg gatcgccctc gagcaggatg
     8581 cggccgtgcg agaggaacag gacgcggtcg cagatctcct gcacctcgaa catgttgtgc
     8641 gaagtccaca gcacgccgca gccgcgctcg gcggcgaatt cgcggatctt cacgcggata
     8701 tcgcgcgccg tggcggggtc gagcgaagcc gtgggctcgt cgagcagcag caggtcggga
     8761 tcgctgacca aggccttggc caggctggcc cgcgtctgct cgccggagga aagcacgccg
     8821 cacttgcggc cgcgcagcgc gtcgaggtcc agcctccgga tcgcgtcgtc cacgcgcgcc
     8881 cggacgtccg gcacgccgta gaggaggccg aagatccgga ggttgcgctc gagggacagg
     8941 ttgccgggca gcggcgcata gacggcggcg aagctcgtgc gctcgagggc cttcgaccgg
     9001 cgcgtcgcga cgtccaggcc ggcgacccgg atcgagcccg cggacggctc cagcacgccg
     9061 aggatcatgt tgatggtcgt ggtcttgccg gcgccgttcg ggccgagcag cccgacgatc
     9121 tcccggcggc cgaccgcgaa cgacacgccg tccacggccg gcgtatcgcc gtagcgcttg
     9181 cgcaggcccg aaaccgacag gacaggggct gccgcgagcc cgctcattcc cggtcgcccg
     9241 tgtcgatgtc ggtctgggcg tggaggtgtt ccgcggcctc cggcgtagcg acaaccttgt
     9301 ggatgcacca gacgagcagg ccgatgaacc cgccgcaggc gagtatcatg atgatccatc
     9361 cggcgggggt catgacggct cctccttgtc ccaggcgcgg ttggccgcgg ccacgatcat
     9421 gctgaagaac gcgaccacgg cggcgatgag cacgaccgag agcagcacca ccggcgggcc
     9481 ctccttcggc agggtgacga tggcctgcca gcgctcgggc aggttcttcc aggcccagag
     9541 ggcgaagatc gcgagcaggt agaccggcga gacgtacttc aggacgaacg gcagggcgcg
     9601 cgggagcctg atttccgccc cgcgccggat ttcctccatg cccttgccga tgcccagcac
     9661 ccaggcgaac acgaccgact ggatcgtggc gaagatgaag atgaagaagt tggccatcca
     9721 gaagtccagc gtgtcgaggg ccgtgaagtc cttcgagaag tacaccacga agaagcagcc
     9781 cgcgagcgtg acgaaggtca ggatgctcac cgaggccctg cgcccgatct tcagcccctc
     9841 ctcgagcagc gagatggagg gctggagcat ggagagcgag ctggtgatgg ccgcgaggaa
     9901 gagcaggaag aagaacagga agccgaagaa cgagcccagc ggcatctggt tgaacacgtt
     9961 ggggagggtc acgaagccca gcccgaacgt gcccggcggg ttctggacca cggcggggcc
    10021 gaggaacacg aaggccgcgg ggatggcgat catgccgccg agcacgacct cgcagaactc
    10081 gttgcccgcg cacgcggtga ccgagctcaa ggccacgtcg tcgttgggct tgaggtacgt
    10141 cgcgtaggtc aggatcaggc cgaagccgac ggagaggctg aagaagatct ggccggtcgc
    10201 ggccagccag atctcgggat tggccagcgc gctccacagg gtctcgcccg gcttggtcgg
    10261 gttccacatg tagccgaggc cgttcaccac cgactgctcc gggtgcgccg ggttggccgg
    10321 cagcgtcagc acacgcccga ggacgaccag cgcgcagact accagcagcg gcatcgccag
    10381 gttgcagaac ttctcgatgc cgcgggtgat cccgcggtag atcaggaacg tgttcgcgac
    10441 gaagcagagg cccaggaaga ggagcatgcg cgccgcggct ggggtgaaca gcgcgccgtt
    10501 cgcccgcgcc ccgacgagcg tcaggaaatg gtcctcgatc accgccagat ccgcgccgag
    10561 ccggccgaag tgccccgtca ggtagtccca cgcgtacgag aggcaccagg cctcgacgaa
    10621 gaggtagtac atgtagatca tcagcggcat gatcgtgccg atcatgccca cgtacgcgcc
    10681 cttgcgggac ccgaggagca tgcggaagat gcccggcgcg gagttgtgcc cccggcggcc
    10741 gccccagcgg ccgagcgccc attccgccat ggccatcggc aggccgacga ccaggaaggc
    10801 cacgaggtag gggatcagga acgcgccgcc gccgtacttc gccgcctggc ccgggaaccg
    10861 caggaaattg cccagcccga cggcgctgcc cgtgaccgcc aggatcgcgc cgacccgcgt
    10921 gccccagtat tcgcgcttgc tcacggccgt gccctcccca tgccgcccgc acgatgttgc
    10981 ccggacgatc gcccccccca cccatcgaca ctaatgactt cccgcttctg ctttagtgtc
    11041 cattagcgga aacaagtggt tacgccttag ccgccacctt cgcccacgta tccttcagtg
    11101 ttacggcgcg attgaacaca agtcggcctg cttttgaatc gggatccacg cagaaataac
    11161 cgaggcgctc gaactggaag cgctcgaggg ggttcgcggc cgcgaggcca ggctcgacga
    11221 acccggttgc cgttttcagc gaatccgggt tgaggaactc gtggtagtcc tttccctcgg
    11281 cggcggcctt cgccatcgga tcctcgacgc tgaacagccg gtcgtacagg cggacctccg
    11341 ccggcacggc gtgcttcgcg ctgacccagt ggatcgtgcc cttgaccttc aacgcggctt
    11401 ccggcggaga ccagcggcag accacggcat cgccctcgac ccgcaggcag gtcaggaaac
    11461 ccgcataccg gatccgcacc gagccgcccg gcgtgagccg gaagtacttc ggcggcgggt
    11521 tctccatgaa gtcatccgcc tcgacgaaca gctcgctgga aaacgggacc tttcgcgtgc
    11581 cggcggattt gtcctcggga ttattcacgg cgtccacttc ggcgggaatt tcactggcgt
    11641 tctcgatgac cacccgccgc gggttcagca ccgccagccg gcgcgccgcc cgcttgttca
    11701 ggtcgtcgcg cacgcagtgc tccagcaacg cgacgtcgga gaggctctcg aacttggtga
    11761 cgccgacggc ttccacgaag gcgcggatgg cctccggcgt atacccgcgg cggcgcagcc
    11821 cgccgatcgt cggcatgcgc gggtcgtccc agcccctcac ccgcccctcg ttcaccagct
    11881 ccagcagccg ccgcttgctc atgaccgtgt aggtcaggtt cagccgcgcg aactcgtact
    11941 gccgcgggcg cgagggcagg ttgtcgaggt tgtcgatcac ccagtcgtag agcgggcggt
    12001 gcacctcgaa ctcgagcgtg caaagcgagt gcgtgatgcc ctcgaaggcg tcctcgagcg
    12061 ggtgcgcgaa gtcgtacgtc gggtagacgc accacgtgtt gcccgtgtgc ggatgcccgg
    12121 cgtgcaggat gcggtacagc accgggtcgc gcaggtgcag gttgggcgag gccatgtcga
    12181 tcttcgcccg gacgcagagc gcgccgtccg cgtgcttgcc ggcgcgcatt tcctcgaaga
    12241 ggcgcaggct ttcctcggcg ggccggtcgc ggtaggggct gggctggccg gccgcgggcg
    12301 gcgcgccgcg gtaggtcttc gaccactcgt cctgcgtcag gccgcagacg taggccttgc
    12361 ccttgcggat cagctcggcg cagcagtcga acatgcggcc gaagtagtcc gaggcgaagt
    12421 agaaatgctt cccccagtcg aagcccagcc agcggacgtc ctcctggatg gactcgatgt
    12481 actcgacctc ctccttggtc gggttcgtgt cgtccatccg caggtggcac tcgccgccgt
    12541 gctcgagcgc catcccgaaa tcgatgcaga tcgccttggc gtgcccgata tgcaggtagc
    12601 cgttgggctc gggcggaaac cgggtgacga cccgccctcc gttcttcccg gcgcgcaggt
    12661 cgtcctcgat gatctggcgg atgaaatgct tcgcgggggc cgcggcttcc gacggctccg
    12721 ggatggattc gtttttcatt tttcgctcga ttccgagcgc gttctatagc ccccgcgcaa
    12781 agaaaaagcg agagctattc cgacgcaggg agccgtcatg aacgggctcg cataactcgc
    12841 ccctccagca tggcgcgccc tggagggcgg agcccggcga cgccgcggcc tacggcgggg
    12901 cgttggtggt cacgagcagg cggtagaaca gcgggtccaa ttccgccgcg ggcgcgtcgg
    12961 tgtaaacgtt gaccggcggc gtggccggga tatggccgtc cagcagcagg aaggggccgg
    13021 gcaggttggt cgcgcggtag atcgcgtaga accggtccgc cgcgctgggc cactgaagga
    13081 cgtacgcccc gggttcggcg gccggctcgg cgccggccga gaacacggag ttggaatcgc
    13141 ccgggtcgaa cccgccgatc cattccccgt agttggacag cccgtcctcg tcgtcatccg
    13201 cctccggatc ggacccggtc gtgccgaggt attcctcctc ccaatcgtcc gggaggccgt
    13261 cggcgtccgc gtcggcatgg aaaacggtcg ccgtgccgag gtagggcaga taattatgcg
    13321 cggtgacggt gatccagagg gccccggccc cctccggcgc gggagacagc gccacggtcg
    13381 cgacgccgtc cgacgcctcg gccgtgccga ggatgcggtt gctgtagacg cagcagacga
    13441 gcgcgccggc ctgggcgacc gccacggtca ccgccgcggc cccggccgca acgcggccgg
    13501 tatgcgccac ggtcaaatcc tgcggcaggc cggtccacat ctccagcgtc ggatcgccca
    13561 ggcagtggaa aatctccatg gtgcatttcg tgtaggagct ggagagggag tacttggtca
    13621 ggaaatacgt cttcgcccgg ttcaggatgt cggccagccg gtatgcgggc gcgcccgccg
    13681 ccgtgtacga cacgccgtgg tactcgagga aattggtcca gacggcgtcg gccatccccc
    13741 agtacagccg gtcgttgtaa tacgagtagc tggtccgcgt cggcgcgagc acgccgatcg
    13801 ccccgccgct ggcgtgccgg acccagtgct cggcgaagca ctcgtcgctg gccgaagttc
    13861 ccgcgccgga atcgtcggtc tcattgtcga accagccggt ctggcagttg atggaccaga
    13921 ccacgggccg caggacgccg ttcgtcaggc cgtccacgtc cgtggccttg gtgaagaccg
    13981 gcttgctcca ccccgaccgg ttcccgtggt cgcggtgggt caggaggaac gtcccgctgt
    14041 tcacggccag ccggatgttc gtccgcgccc cgctccacgg gaacgccggc ttgagcagct
    14101 ccgccggcag cgcgacgccg cccaggggcg ggctgtcgtt ctcgaagagg gcgctgtacg
    14161 aggtggccca gttggcccag tgcgtcgggt tggtcccctg gtattccgcg tagatccgct
    14221 cggcggcgta cccggcgtcc gcgaggaagt tctggaggtc ctccgtcgat ttcacgaacc
    14281 ggcgctccga atacccgtag taggcggtgc ccgtgtagcc ctggaacgag gcgcagaggg
    14341 tggcgttggt gtaatagcgg ccgaaccgcg agcggtcggg cggcgcgctt tcgtaggcga
    14401 tgacgcgcgc ggcccagttc gtcgcctggg ccgcggtctc caccggccag cgcccgatgg
    14461 ccaggtccgg gatccagtcg agcgacgcgc tcatgtcggc atagaacagg tccgtgctgt
    14521 tcttccgctc gccggagggg tggtccgtct tgtaccaggg cgggatgaag tccgagtcgc
    14581 cgatcagcag cacgtaggcc ggcgccgggc tccacgtggt gtacgcgttc aggatgtagt
    14641 cgcggacctg attggtggcg ctgccggtct cgtttgtcgt tacgacggtc gtcgcgatcc
    14701 cgcgccgcat cttccactcc gccagctggt aggcggcgtt ggtgaaggcg tcggcgcaga
    14761 tgatcagcag ctcgcagccg ttcgtcgagc cgccgaaggc cgcgctgccg cgtcggggcg
    14821 ccggggcggc atccagaatt tccgccgcgt tcggagcgcc ccgcaacaag ccgtcgaaag
    14881 cacggctcgc cagccgcgga tcgcgccgct ccggcgtccc cacaaattcc agccgcaccg
    14941 cgaggcgggg atacaccgtc aggacgcccg atcgcgggtt gtactggtag ggatggagcc
    15001 ggaccaggcg aaccgcctgc ccgcgtattt cactcacggc ctcgaacgac gcgaacacgc
    15061 cggggtagtc ggcatcccgc gcatacaccg cctcgtcgcg ggccatcggg gcggaaccgc
    15121 ccgagtcgga gggcggcggc tgaacgggcg ccagctggac gccggtgaat atttccgggg
    15181 cgccgggcgc cgcctccacg cggacctcgc aaccgttcgg gaccaggatc cattgggcga
    15241 aagccggcac gtcaggctgg ccgatgggtc cgctggcgcc gcccgggtcc agcgccaggt
    15301 cgtacgcgcg gccgtccggc aggactgccg atccgcggac gaaaccggca agttccaccg
    15361 ccagcgtcag gcccgccgcg ctcgagtcgc gaatgcgcac ctgcccggcc gtgggcaggc
    15421 cgccgtccag cggcacccct tcgcggcccc gcgccccggc gggctgcagg gccatcagca
    15481 tccagaacac ttggcgggca tatcggaaca aggccgtctc ccgggcgggg cccgcaccgc
    15541 gaagggcccc gcgcctatta cgtataagta accgggaaac agggggatgt ttagggatca
    15601 gatattcaac ccgaagaaca gccaccagat ggcgaaggcc acggccgcca tcggagggcc
    15661 gtgcaggaac aggagccgcg cggcctcatg ccgacgcacg ttccagagcg agagggccgt
    15721 aaccaggccg aacaggttgg ccaagcaggc caggggacag agcatcttca gggcccatcc
    15781 gatcatgggc caggagtaat gccagtcttt gtgcaggaac aggcccagca cagcccagta
    15841 tgccaacgcc ccccacccca gccatctcgc cataaaccct ccgcccgagc acgctccaga
    15901 aacgtccccc ctactgcagg agatcgaaca cgctgccgac ctcgtgcctg atgccgcaat
    15961 acatgcagat gtcccgcccg cgctccaggg gccgcccgca tttgggacag ggcggcgccg
    16021 gcgcgccggg acgggtcgcg gcggactgct ccgaggccat ttgccgcaga cgatccttga
    16081 gctccttctc cgtcaggttg gcggcctcgc tcaagatatc ccaaatggcc cgattcagcg
    16141 gaaccaggcg cgccacgatg cgctccaacc gctcgaggcg ctgcgccatg ccctcggcct
    16201 tggccgcaat gaactcggcc ttctcctcgg ctttctgggc cgaccggaag ttttccatcg
    16261 cgctgaaaag gttgtccatc ggccgtcctc caggatcagg atacctgctt gcgcactact
    16321 ataccccctc ggcgcccgcc tgcgcagggc aattctccgc tccccgcttc tgcgccaccg
    16381 ccatgagttc catctcgtcg agctgaatcg ggccccggtt ccaagccccg gccgtcccgc
    16441 cccagatgtg c
//
LOCUS       DAOOTK010000066        16323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2432_length_16323_cov_4.697689, whole genome shotgun sequence.
ACCESSION   DAOOTK010000066 DAOOTK010000000
VERSION     DAOOTK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_16323_cov_4.697689"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..8186
                     /locus_tag="PK634_08595"
     CDS             12..8186
                     /locus_tag="PK634_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOW97832.1"
                     /translation="MRITGLGNGSSGYAERSLGAAINSGQIYVAFMMAYQYYGSNKWA
                     GLSFYNGATEKAFFGKASGANWYTLGVAGDGGTAWSGYDLRPYGGGAEAHTGSVYLVL
                     GKYDFSTKNLQANAYNLPTATEFPSSEPTSWDVTRTLGTGIDSVDRIRLNVGALNPDC
                     GSIGDVYFDEIRLATNWSQLVGVTCPTWIGSNNVPVAVTNLGNSLTFECQTYPIGSGQ
                     SADLQIRWTDQTTSTYSMAWSSNHNNNSWWSYSAQMTKTGVHDFVYLAYGGSCSVIST
                     NPSGSLTVNALVNPNPVSATRDSVNTNSQINLAWTKNTVGHDVMIVRNTSGSFTAPTP
                     GTAYYAGETLGSDTVVYRGNASSFDDTGLASSQTYYYRFYSENYSYYSTGTVDSASTL
                     GGSQAIVVDGNPADWQGTAGSTYNASTVSRNEFIWKDKCGEERKDVANTTNADIREVR
                     IYADTTNVYFLVKYGDVTSDQHPYINIGLDTRRSSASGAMNWIGDDSGTFYGGNYFAG
                     GQAAVHYAQRNITVHRADGAMQVEVYADDGGTWYGPPAGSAAAASAANNAMEFWINRS
                     DLLVSSPVTARFTVASFLNNDLWNNSGDGTVQILAGTPDAADAASIAPEQVNDGDLSQ
                     SAWQEDISDNSIDFWFDVVFDANGVVANSIPSTPAVGFPTNNYAATANPTLWWNASTD
                     SDGQVTGYFLEVGTNETLNGESGTTENGSIFLRVNLPATQTNYTLSTSATQYWWRVRA
                     RDTAGALSSGQIQMFRIGGKGDITGPLHTLLYIGTNLTGYLAGDFDAHIDKYGPIQSI
                     TDAEIQQAVNAIGFAIKIEDPSGVYATNRQNNSGPAPSNFTWNIVSDDGRVSPNWDVF
                     RLYSDGSSNELGYDAVFYGSNIASSAGNSSPSITAWVHNAFTISGYDETIEYYLTVSS
                     EDGCTENGSWAAYGSWNSYGAPAATYGGYCEDGPNTARNVSTNELIRINVTDDDDDVP
                     TFAYGAQWASSRTLLASNATALLTASGSGQSVIYTGTDGGMTGTPLTLLFNAYDYYSG
                     LQLSAAGSADTNTSLSVGSSSWQTNNIANYSASRSTVGDTTETNTVLAWHWDSLAFSD
                     MTALWAGDGSGSAGETNPVMLTLRDTDHDRTDDASVASNVVFGYLQVTDDDSVAPSHT
                     QFTIDGVGGYGSTNLLPGNIAVVGVNGSPSAKIEKFAFVVLAPFPAGTIVHFTDCGWA
                     DNAGTNWHRVTEFHTNLWIATGDADVGQVFELGLQDINSGGDQVTVYQYNGSVNASND
                     PDRVTFLYAVNMLGTNTAPQADGWYLPIVPTNNLCSALYRGLTNGVTAVSVGGAESVN
                     IIYTGRLTGSSSELLASISDSNNWQVAASATDLNFSNFAFSVTSQGGMEWQTPNLTDA
                     QVSTGQYTVVDVVSDYTSGLLATNNAYASAPHFLLYNADGDMALSNAMSVGFPNGVPV
                     TTQALTRTASTGMWNYINIGTYSAVVYVTDFDFDRLNDNQDGSLAMPVAVTDDDADAP
                     LFTNLTALGTGAGLTENGTMVLYDFDIPVDETTYTFTTNADTVVAHVTAGAVLGGGVD
                     AINQGTGNPGYCAVTSKFYQAYQYLDFTLTVESGYALTVTNLTFMDNRSSTGPTDWYF
                     LYSGDSYATALASGNHADTNWKTNSTALSLSSLEGAVTFRLHATNATGSTGSWRVDNL
                     RLQGSLAPLPGSGLVSDQDLNQGTLSITARVTDATSGVYSNRHPTYGTRFDLVTPAGA
                     SLFNQQPFNIGPTNASQTIGEWANLYATNLSVAFANIVLGVHTASVYAADADNDRPDD
                     NLQYTQQIAVTVIDDDTNPPVGGTIFQSMTKDKPFDYPNLHGPMVLYQGTTNASAAGV
                     STNRRWSLNDRMMQNVGSTNTPYLHINLYDESGYHRSGTEALSNLTLTIEGFITNDVS
                     YLSLASSSASTTNNPAATNVWVFTSFGDMSFVGTTSKVTVSVSDTDKDRPDDASVLTA
                     YQVGYIAWKDNDPSPAILQFPTNQTANTNALTVLLGPSPGAPTSNLWTYSTHPEDASN
                     QVYAVSDGELAGVSGSSQLMLGFHSYDLGDDNVLGLQRDTTATTTSDGRTLTNTHISI
                     GSVVSSNTANYSSGWSSSIGETRIAAQNATSYWAFTTFTSNQVGQFWDAAGHSNAIVL
                     HALDSDNDRPGDQARTNLNAGWLVVYDDDTNAPTAPTALSVSPTDWTNANSFTLSFTD
                     SSDASGILHYRYDTNAPASLTNGTEMPVSRIITGVAEGEITNRLFAVDADHDRAFDGQ
                     MSETTNFVTRLDQHAPPQIMGITVDEGEDPTTEVELNWTAAANAGNRPDGATLSPWNS
                     YRIYFSEDGSTVTTSSAYFDGYTGQAALTNMSVSTVTLSNLIFGSDYKFRIVGRDRAG
                     NEGPLSDEVVIVLPGFNVTQGLVKATSGDIRYSEIFWVAATNQSGGVNREYDLLWVDG
                     SSFNDGMSNQWQFLQSGFTNSLSDTGGLNRTAPTLLTSNMRFYRAASKDRWMTNRSPR
                     VASEEVYGLRTVTLYPGQNWVGLPVVPDSNTLKNVLGHYLPGGSAYTDPHSSIVTWYS
                     RLTNETVQKDVYLLDWGSSNEWRTGQGWGGMNQVADHYAFPFDESVAINIPTNETQTY
                     LLMFIGRVPTNNQTQVIQPNGAYNLVNMRLPCTMSPAQMNLLASGFKGGVGSMSADRI
                     RKLNRQQQGVGFDVYYNTTRSRWEYVNNSSAAGFTISPDDGFLIWTRGSTTPWTWTNV
                     PPYSLPTRLMNP"
     gene            8319..9410
                     /locus_tag="PK634_08600"
     CDS             8319..9410
                     /locus_tag="PK634_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOW97833.1"
                     /translation="MSKPRLVRFEISTRAPLPAGEQVFVAGSQEVVGSWRPDGLPLTR
                     ASDDLWVGAAACSSREPLEFKITRGSWDTEEVGDDGKIPENHRLEPGGEAVVRRTVLR
                     WRDAARARTPKITGSYRVHEGFRSAFLRFDRTVIVWLPPSYAQQPDRRYPVCYLQDGQ
                     QVFDPQTSSGNQDWEVDEWCARLMAAGEMEEIIAVAVYSTADREPEYHPSLAGASYAR
                     FLVEELKPWVDREYRTLPGAASTAVAGASLGGTISFFLAWSRPDVFSAAACLSPAFRF
                     QGDRFCPDLVRNAAAVPGIRLFLYCGLGDPLERELAEGMSEMLALLKTRGVEPGARLA
                     VVEDLEGRHHEESWARHTGAWLRFLYGKG"
     gene            9414..9884
                     /locus_tag="PK634_08605"
     CDS             9414..9884
                     /locus_tag="PK634_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97834.1"
                     /translation="MPGRKVKRQRPGGPLAYIAFWQFMAFLLLVSLIWADAVLDLPNL
                     LFRLPEGRFNWYRACVLTAAVIVVGFVSIGHAYVQQKRALKGLIKICSYCRRVQVDEQ
                     AWRRMEDFIETRTLAEFTHGVCPECYQRALEGLSGPPPGAPPPESTLDRGPSSP"
     gene            9982..10695
                     /locus_tag="PK634_08610"
     CDS             9982..10695
                     /locus_tag="PK634_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97835.1"
                     /translation="MVAKTEAKPVAENDGDKNEAVVPYALLFVLEDLAVNGRQVLSDA
                     LRRHAQESKEKFDDRLFMRHGLTGSPAASLPAILGGDAESQKAVQQRVLDKLAADKAG
                     LNPALASVLKAAAQRRIPALALTSLPEDAARAIAGRLGLEEMGVTLFLVKDAAADFPT
                     PDTWVKAARSIQRRPRGCGALAASGRVARGAVMAGMSCVALPDELTVFEDFGGTALVI
                     ETPGDASPSEIIGSLFRQI"
     gene            10722..11516
                     /gene="mazG"
                     /locus_tag="PK634_08615"
     CDS             10722..11516
                     /gene="mazG"
                     /locus_tag="PK634_08615"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOW97836.1"
                     /translation="MVQAKDPGGKPIDRLREVMRMLRAENGCPWDREQTLQTLKPFLL
                     EECYEAMDALESGDRARLCDELGDVLLQVVFQAQVASEEGSFTFDDVATAISDKLVRR
                     HPHVFGEVQVSDSAEVIRNWEAIKKGEKGPPRKSAVDGVPRSAPALHKAQQIQKRAAR
                     VGFDWDTVHQVMAKVEEEVGEVREAMAGGDPKKVKEEIGDLLFAAVNLSRFLGHSAEE
                     ALEDTVRKFTRRFQGIEERLHAQGRKMTECKLAELDAIWNEIKAAE"
     gene            11718..12995
                     /gene="nusA"
                     /locus_tag="PK634_08620"
     CDS             11718..12995
                     /gene="nusA"
                     /locus_tag="PK634_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOW97837.1"
                     /translation="MNNDLLAVIDYMERERGIDRETLLTAVEAALLSASKKSVGPSKD
                     LRIEIDRKTCRIRALAKVTVAEKVNAPHDEITLPSARRIKADAQLGDTVEIEVTPKNF
                     GRIAAQTAKQAILHKIRQAEKERVFGEYKDRAGDIVSGTVSRIERSDVILDLGRAEGL
                     MPVAEKVPTERYQIGDRLRALILKVESRTQGPEIILTRSHPDFVRRLFELEVSEIADK
                     TVEIKGIAREAGFRSKIAVVSHDPKVDPVGACVGMRGIRVKNIVRELSGEKIDIVRWH
                     DDIKTFVTNALAPAKLIRVTVDTEAQSVQVVVDAEHLSQAIGKKGQNARLTSKLTGWK
                     IDILRDESDVSFEEKVARAVAALAVVDGIGPERAEKLVHAGFLTLEGLLAAEPADLEA
                     VEGIDAAAAREVLQGVEKACEHLHGQEESHESS"
     gene            12982..15048
                     /gene="infB"
                     /locus_tag="PK634_08625"
     CDS             12982..15048
                     /gene="infB"
                     /locus_tag="PK634_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOW97838.1"
                     /translation="MRVHELAKELGLTSKELMDRLHAMSVEVRNNFAALSPEQVALVR
                     KAVGAPAAAAAPAETGAAKPAPKAAPPAKAAPAPAKPAAPPPPAPAAPPPPPPPPAPK
                     VIIVKGPIIVKEFAEQLGLKPNLLIAELMTMNVFASINERIEFKVAQQLASRHGASIE
                     HEKKAPEPKPVKKVEPKVEEPRPEDIQPRPPVVTFLGHVDHGKTSLLDRIRHSKVVQS
                     EDGGITQHIGAYTVTYRDHPITFLDTPGHAAFTAMRARGANLTDIAVLIVAADDGVMP
                     QTKEAIQHARAANVCIMVAINKIDLKSAVVDRVKRQLQHEGLAPEDWGGQTICCPVSA
                     VTGDGIDHLLEMILLQSEMLELKGNARRPAQGFVIEARLEAGSGPTANLLVKQGTLSV
                     GDAIVCGRCWGRVKALINDQGIKVRTAGPSIPVKCLGLNGVPEPGMEFVVYPNDREAR
                     AIAEEREAASRLQAISAPRRASLDDLLKPAETQQRTELAIVLKADVQGSLEAIRQALS
                     GIKSDKVLLRIALAGVGNITGNDVLLASASNAIVVGFHVSKEDGVAALAKREGVEIRL
                     YSVIYQLLDEVRDAMVGLLEPVIKETVIGHAEIRQVFEIGKKGNVAGCMVTDGRITSH
                     ARARVKRADDVLYEGAVVSLKRFQDDASEVREGLECGIRLDRFTDFKPGDVIECYEVQ
                     KIEQPL"
     gene            15096..15488
                     /gene="rbfA"
                     /locus_tag="PK634_08630"
     CDS             15096..15488
                     /gene="rbfA"
                     /locus_tag="PK634_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOW97839.1"
                     /translation="MSHQRMVRVNELLKREIADALFRVMNESGFDLTAVTVTGVEVGS
                     DLRTAVVRVSIRDHRAERGDMLKRLYRHRGEIQEILHRHVILKYTPRLSFELDESIEK
                     GDRVLHILHKMENAGELGAGGAGGSPAT"
     gene            15485..16237
                     /gene="truB"
                     /locus_tag="PK634_08635"
     CDS             15485..16237
                     /gene="truB"
                     /locus_tag="PK634_08635"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOW97840.1"
                     /translation="MSGGRYAAPPPVIEPWEGLLLVDKPAGMTSHDVVDAIRRRFGFR
                     KVGHGGTLDPMATGLLVILLGRGTKLSDRVMASDKTYEGTLKLGATTDTEDVDGQILA
                     EAPWEHVTREAVEREMAARVGDLMQTPPMVSAVKKQGVPLYKLARKGQTVEREPRLIH
                     VYEFRLLAFEPPRARFVLRCTKGTYVRTLCADIGRALGCGAFLEALRRTRAGALAVEA
                     AAPLDDILRMDVDRVAERVLPLHGFLAGGSPG"
BASE COUNT         2946 a         5533 c         5492 g         2352 t
ORIGIN      
        1 cgggcaacct gatgcggatc accggcctcg gcaacggctc gagcggctac gccgagcgca
       61 gcctgggcgc ggcgatcaac tccggccaga tctacgtcgc cttcatgatg gcctaccagt
      121 actacggctc gaacaagtgg gccgggttga gcttctacaa cggcgcgacc gagaaggcgt
      181 tcttcggcaa ggccagcggc gccaactggt acaccctggg ggtggcgggc gacggcggaa
      241 ccgcgtggtc cggctacgat ctgcggccgt acggcggcgg cgccgaggcg cacacgggca
      301 gcgtctacct cgtgctgggc aagtacgact tcagcacgaa gaatctgcag gccaacgcgt
      361 acaacctgcc gacggcgacg gagttcccga gcagcgagcc gacgagctgg gacgttaccc
      421 ggacgctggg cacgggcatc gattccgtgg accgcatccg gctgaacgtc ggcgccttga
      481 acccggactg cgggagcatc ggcgacgtgt acttcgacga gatccggctg gcgacgaact
      541 ggtcgcagct ggtcggcgtc acctgcccca cgtggatcgg cagcaacaac gtgccggtgg
      601 cggtgacgaa cctcggcaat tcgctgacgt tcgagtgcca gacctacccg atcggctccg
      661 gccagagcgc ggacctgcag atccgctgga ccgaccagac cacctcgacc tactcgatgg
      721 cctggtcgtc gaaccacaat aacaacagct ggtggagcta cagcgcgcag atgaccaaga
      781 ccggcgtgca cgacttcgtg tacctcgcct acggcgggag ctgcagcgtc atcagcacca
      841 atccgtccgg cagcttgacg gtcaatgccc tggtcaaccc gaatcccgtc tcggccacgc
      901 gcgactcggt caacaccaac agccagatca acctggcgtg gaccaagaac acggtgggcc
      961 acgacgtgat gatcgtccgg aacacgtcgg gcagcttcac cgcgccgacg ccgggcacgg
     1021 cctactacgc gggcgaaacg ctcggctcgg acacggtggt ctaccgcggc aacgcgtcca
     1081 gcttcgacga caccggcctg gcgtcctccc agacgtacta ctaccggttc tactccgaga
     1141 actacagcta ctactcgacc ggcacggtgg acagcgcctc gaccctgggc ggcagccagg
     1201 ccatcgtggt ggacggcaac ccggcggact ggcagggcac ggcgggctcc acctacaacg
     1261 cgtcgaccgt gtcgcgtaac gagttcatct ggaaggacaa gtgcggcgag gagcgcaagg
     1321 acgtggccaa cacgaccaac gcggacatcc gcgaggtccg catctacgcg gacacgacca
     1381 acgtctactt cctggtgaag tacggggacg tcacgtccga ccagcacccg tacatcaaca
     1441 tcggcctgga cacgcggcgg tcctcggcga gcggcgcgat gaactggatc ggcgacgact
     1501 ccgggacctt ctacggcggc aattacttcg ccggtggcca ggccgccgtg cactacgcgc
     1561 agcgcaacat cacggtgcac cgcgccgacg gcgccatgca ggtggaagtg tacgccgacg
     1621 acggcggcac ctggtacggc ccgccggccg gctcggcggc ggcggccagc gcggcgaaca
     1681 acgccatgga gttctggatc aaccgctccg acctgctggt cagcagcccc gtcacggccc
     1741 ggttcacggt cgccagcttc ctgaacaacg acctgtggaa caactcgggc gacggcacgg
     1801 tccagatcct cgcgggcacg ccggacgccg ccgacgcggc ctccatcgcg ccggagcagg
     1861 tcaacgacgg cgacctctcg cagtcggctt ggcaggagga catcagcgac aacagcatcg
     1921 acttctggtt cgacgtcgtc tttgacgcca acggcgtggt ggccaactcg atcccgagca
     1981 cgccggcggt cggcttcccg accaacaact atgccgcgac cgccaacccg acgctgtggt
     2041 ggaacgcctc gaccgacagc gacggccagg tcaccggcta cttcctggag gtcgggacga
     2101 acgaaacgtt gaacggcgag agcggcacga cggagaacgg ctcgatcttc ctccgcgtga
     2161 acctgccggc gacccagacg aactacaccc tctccacgtc ggcgacgcag tactggtggc
     2221 gcgtccgggc gcgcgacacg gccggcgcgc tctccagcgg ccagatccag atgttccgca
     2281 tcggcggcaa gggcgacatc acggggccgt tgcacaccct gctgtacatc ggcaccaacc
     2341 tgacgggcta tctagccggg gatttcgacg cgcacatcga caagtacggg ccgatccagt
     2401 ccatcaccga cgccgagatc cagcaggcgg tcaacgcgat cgggttcgcc atcaagatcg
     2461 aggacccgag cggcgtgtac gcgacgaaca ggcagaacaa cagcgggcct gcgcccagta
     2521 acttcacgtg gaacatcgtc tccgacgacg gccgcgtgtc gccgaactgg gacgtgttcc
     2581 gcctctattc ggacggctcc tccaacgaac tgggttacga cgcggtgttt tacggcagca
     2641 acatcgcctc gtcggcgggg aactcctcgc cgagcatcac ggcctgggtg cacaacgcct
     2701 tcaccatcag cggctatgac gagacgatcg agtactacct gaccgtcagc tcggaggacg
     2761 ggtgcaccga gaacgggagc tgggcggcgt atggctcgtg gaacagctac ggggcgccgg
     2821 ccgccaccta cggcggctac tgcgaggacg ggcccaacac ggcgcgcaac gtcagcacga
     2881 acgagctgat ccggatcaac gtcacggacg acgacgacga cgtgccgacg ttcgcctacg
     2941 gcgcgcagtg ggcgagcagc cgtacgctcc tggcgtccaa cgccacggcc ctgctgaccg
     3001 cctcgggctc cggccagtcc gtgatctaca cgggcacgga cggcggcatg accggcacgc
     3061 cgctgacgct gctgttcaac gcctacgact actacagcgg cctgcagttg agcgccgcgg
     3121 gctccgcgga cacgaacacc tcgctctccg tcggttcctc gagctggcag accaacaaca
     3181 tcgccaacta cagcgccagc cgcagcacgg tgggcgacac gaccgagacc aacacggtcc
     3241 tggcctggca ctgggactcg ctggccttca gcgacatgac ggccctctgg gccggcgacg
     3301 ggtcgggctc cgccggcgaa accaacccgg tgatgctgac gctgcgcgac accgaccacg
     3361 accggaccga cgacgcgagc gtggcctcca acgtcgtctt cggctacctc caggtcacgg
     3421 acgacgactc ggtcgccccg agccataccc agttcaccat cgacggcgtc ggcgggtacg
     3481 gcagcaccaa cctgctgccc ggcaacatcg ccgtcgtcgg cgtcaacggc tcgcccagcg
     3541 ccaagatcga gaagttcgcc ttcgtggtcc tggccccgtt cccggcgggg acgatcgtcc
     3601 acttcaccga ctgcggctgg gcggacaacg cggggaccaa ctggcaccgc gtgaccgagt
     3661 tccacacgaa cctgtggatc gcgacgggcg acgcggacgt cgggcaggtg ttcgaactgg
     3721 gcttgcagga catcaactcg ggcggcgacc aggtcaccgt gtaccagtac aacggctcgg
     3781 tcaacgcctc caacgatccg gaccgcgtca cgttcctgta cgccgtcaac atgctcggca
     3841 cgaacaccgc gccacaggcg gacgggtggt acctgccgat cgttccgacc aacaacttgt
     3901 gctcggccct gtaccgtggt ttgacgaacg gcgtgaccgc cgtcagcgtg ggcggtgccg
     3961 agtcggtcaa tattatctac acggggaggc tgaccggctc gtcctccgag ttgctcgcct
     4021 caatcagcga ctccaacaac tggcaggtgg cggctagcgc aacggatctg aatttcagta
     4081 acttcgcgtt cagcgtcacc agccagggcg gcatggagtg gcagacgccg aacctgacgg
     4141 acgcgcaggt gtccacgggc cagtacacgg tggtggacgt ggtgtccgac tacacctccg
     4201 gcctgctggc cacgaacaac gcctacgcga gcgcgccgca cttcctgctg tataacgcgg
     4261 acggcgacat ggcgctgtcc aacgcgatgt cggtgggttt cccgaacggg gtgcccgtga
     4321 cgacccaggc cctgacgcgg acggcatcca cgggcatgtg gaactacatc aacatcggca
     4381 cgtacagcgc ggtggtctac gtcacggact tcgacttcga ccggctcaac gacaaccagg
     4441 acggctcgct ggccatgccg gtggccgtca ccgacgacga cgccgacgcg ccgctgttca
     4501 cgaacctgac ggccctcggt acgggcgccg ggctgaccga gaacggcacg atggtgctgt
     4561 acgacttcga catccccgtc gatgaaacga cgtacacgtt cacgaccaac gcggacacgg
     4621 tcgtcgccca tgtcaccgcc ggcgcggtcc tcggcggggg cgtggacgcc atcaaccagg
     4681 gcacgggcaa tccgggctat tgcgcggtca ccagcaagtt ctaccaggcg taccagtacc
     4741 tcgacttcac gctgacggtg gagtccggct acgcgctgac ggtgacgaac ctgacgttca
     4801 tggacaaccg ctccagcacc ggccccaccg actggtactt cctgtacagc ggcgacagct
     4861 acgcgaccgc cctggccagc ggcaaccacg ccgacacgaa ctggaagacg aacagcacgg
     4921 cgttgagcct gtcgtcgctg gagggcgccg tcaccttccg cctgcacgcg acgaacgcga
     4981 cgggctcgac cggatcgtgg cgcgtggaca acctgcggct gcagggcagc ctggccccgc
     5041 tgccgggctc cggcctggtc agcgaccagg acctgaacca gggcacgctg tcgatcaccg
     5101 cgcgcgtcac ggacgcgacc agcggcgtct acagcaaccg ccacccgacg tacgggacgc
     5161 ggttcgacct ggtgaccccg gccggcgcct cgctgttcaa ccagcagccg ttcaacatcg
     5221 gcccgaccaa cgccagccag accatcggcg agtgggcgaa cctctacgcg acgaacctgt
     5281 cggtggcgtt cgcgaacatc gtgctgggcg tgcacaccgc cagcgtgtac gcggcggacg
     5341 ccgacaacga ccggccggac gacaaccttc agtacacgca gcagatcgcc gtgacggtca
     5401 tcgacgacga caccaatccg ccggtgggcg ggacgatctt ccagagcatg acgaaagaca
     5461 agccgttcga ttacccgaac ctgcacgggc ccatggtgct gtaccagggc acgaccaacg
     5521 cctcggcagc gggcgtctcg acgaaccggc gctggagcct gaacgaccgc atgatgcaga
     5581 acgtgggcag cacgaacacc ccgtacctgc acatcaacct gtacgacgag agcggctacc
     5641 accgcagcgg gacggaggcc ctgtcgaacc tgaccctgac catcgagggc ttcatcacga
     5701 acgatgtctc ctacctctcc ctggcctcga gcagcgccag cacgacgaac aaccccgcgg
     5761 cgaccaacgt ctgggtcttc acctcgttcg gggacatgag cttcgtgggc acgacgagca
     5821 aggtcacggt gagcgtctcc gacacggaca aggaccgccc ggacgacgcc tcggtgctga
     5881 cggcctacca ggtcgggtac atcgcctgga aggacaacga cccgtcgccg gcgatcctgc
     5941 agttcccgac gaatcagacc gcgaacacga acgcgctgac cgtgctcctc gggccgagcc
     6001 ccggcgcccc gacgtccaac ctgtggacct attccacgca cccggaagac gcgtccaacc
     6061 aggtgtatgc cgtgtccgac ggcgaactgg ccggggtgtc cggatcgagc cagctgatgc
     6121 tcggcttcca cagctacgac ctcggcgacg acaacgtcct gggcctgcag cgcgatacga
     6181 ccgcgacgac cacttcggac gggcgcaccc tgacgaacac gcacatcagc atcggctccg
     6241 tggtgtccag caacaccgcg aattacagct cgggctggag ttcgtccatc ggcgagaccc
     6301 gcatcgccgc ccagaacgcg accagctact gggcgttcac cacgttcacc tccaaccagg
     6361 tgggccagtt ctgggatgcc gccggccaca gcaacgccat cgtgctgcac gcgctggatt
     6421 cggacaacga ccggccgggc gaccaggccc gcaccaacct caacgccggc tggctggtgg
     6481 tgtacgacga cgacaccaac gcgccgaccg cgcccacggc gttgagcgtc agcccgaccg
     6541 actggacgaa cgccaactcg ttcacgctgt cgttcaccga ttccagcgac gcgtccggga
     6601 tcctgcacta ccggtacgac acgaacgcgc ccgcttcgct gaccaacggc acggagatgc
     6661 ccgtgagccg gatcatcacc ggcgtggccg aaggcgagat caccaaccgg ctcttcgccg
     6721 tggacgccga ccacgaccgc gccttcgacg gccagatgag cgagacgacc aacttcgtca
     6781 cgcggctgga ccagcacgcc ccgccgcaga tcatggggat cacggtggac gagggcgagg
     6841 acccgaccac ggaggtcgag ttgaactgga ccgccgcggc caacgccggc aaccggccgg
     6901 acggcgcgac gctgtcgccg tggaactcgt accggatcta cttcagcgaa gacgggtcca
     6961 ccgtgaccac gagcagcgcc tacttcgacg ggtacacggg gcaggcggcc ctgacgaaca
     7021 tgtcggtctc gacggtcacc ctgtcgaacc tgatcttcgg ctccgactac aagttccgca
     7081 tcgtcggccg cgaccgggcg ggcaacgagg ggccgctgtc ggacgaggtg gtgatcgtgc
     7141 tcccgggctt caacgtcacg cagggcctgg tcaaggccac cagcggggac atccgctact
     7201 cggagatctt ctgggtggcc gcgacgaacc agtccggcgg ggtgaaccgc gagtacgacc
     7261 tgctgtgggt ggacggcagc agcttcaacg acgggatgtc caaccagtgg cagttcctgc
     7321 agagcgggtt caccaactcg ctgtccgaca cgggcggtct gaatcgcacg gcgccgacgc
     7381 tgctgacctc caacatgcgc ttctaccgcg ccgcctccaa ggaccgctgg atgaccaacc
     7441 gctcgccgcg cgtggccagc gaggaggtgt acggcctgcg gaccgtcacg ctgtatcccg
     7501 gccagaactg ggtcggcctg ccggtggtcc cggactccaa cacgctgaag aacgtgctgg
     7561 gccattacct gccgggcggc tcggcgtaca ccgatccgca cagctcgatc gtgacctggt
     7621 acagccgcct gaccaacgag acggtgcaga aggatgtcta cctcctggac tggggctcct
     7681 ccaacgagtg gcgcacgggc cagggctggg gcggcatgaa ccaagtggcc gaccactatg
     7741 cgttcccgtt cgacgagagc gtggccatca acatcccgac caacgagacg cagacctatc
     7801 tcctcatgtt catcggccgg gtgccgacga acaaccagac gcaggtcatc cagccgaacg
     7861 gagcgtataa cctcgtgaac atgcgcctgc cctgcacgat gagcccggct caaatgaacc
     7921 tgctggcctc cggtttcaag ggcggcgtag gctcgatgtc cgcggaccga atccgcaagc
     7981 tcaaccggca gcaacagggc gtcggctttg acgtgtatta caacaccacg cgctcgcggt
     8041 gggaatacgt caacaactcg tcggcggccg gcttcaccat ctcgccggac gacgggttcc
     8101 tgatctggac gcgcgggtcc accacgccgt ggacgtggac caacgtgccg ccgtacagct
     8161 tgcccactcg cctgatgaat ccgtagcggg cggccccggg tggcgttcgc gaggcgggcc
     8221 ggcggcggcc cgcctcttct tttttgccgt ctgcgggcta gccatgccgg cggcgttggt
     8281 ccataatggg cccgggcgaa aatccggagg cgctggccat gtcgaaaccc aggctcgtac
     8341 gattcgagat cagcacccgc gcgccgctcc cggccggcga gcaggtgttc gtcgccggca
     8401 gccaggaggt cgtcggttcg tggcggccgg acggcctgcc cctgacgagg gcctcggatg
     8461 acctgtgggt cggcgcggcc gcctgttctt cccgggagcc cctggaattc aagatcaccc
     8521 ggggctcgtg ggataccgag gaggtcgggg acgacgggaa gatcccggag aaccacaggc
     8581 tggaaccggg cggggaggcc gtggtgcggc gcacggtcct gcgctggcgg gacgccgccc
     8641 gggcccggac gccgaagatc acggggagct accgggttca cgagggattc cgttccgcgt
     8701 tcctccgctt cgaccgcacc gtcatcgtct ggctgccgcc gtcctatgcc cagcagcccg
     8761 accggcgcta cccggtgtgc tatctccagg acggccagca ggtgttcgac ccgcagactt
     8821 cgtccgggaa ccaggactgg gaagtggacg agtggtgcgc gcggctgatg gccgccggcg
     8881 aaatggagga gatcatcgcc gtggccgtct attccaccgc cgaccgggag ccggagtatc
     8941 atccatcgct ggccggcgcg tcctacgcgc ggttcctggt cgaggagctc aagccgtggg
     9001 tggaccggga gtaccggacg ctgccggggg cggcctccac ggccgtggcc ggcgcgtcgc
     9061 tgggcggaac catctccttc ttcctcgcgt ggagccggcc cgacgtgttc tccgccgcgg
     9121 cctgcctctc cccggccttc cgtttccaag gcgaccgttt ctgcccggac ctggtgcgga
     9181 acgcggcggc ggtccccggc atccgcctgt tcctttactg cgggctgggc gatccgctcg
     9241 agcgggaact ggccgagggg atgtccgaga tgttggcctt gctgaaaacg cggggcgtgg
     9301 agccgggcgc gcggttggcc gtggtggagg acctcgaggg gcggcaccac gaggagtcct
     9361 gggcccgcca taccggcgcc tggctccgct tcctgtacgg aaaggggtag ggtatgcccg
     9421 ggcggaaggt caagcggcag cgcccaggcg ggccgctcgc gtatatcgcc ttctggcagt
     9481 tcatggcctt tctcctgctg gtcagcctga tctgggcgga cgcggtgctg gacctgccga
     9541 acctgctttt ccggctgccc gaaggccggt tcaactggta ccgcgcctgc gtgctgacgg
     9601 ccgccgtgat cgtggtcggc ttcgtctcga tcggccacgc ctacgtgcag cagaagcggg
     9661 cgctcaaggg cctcatcaag atctgctcct actgccggcg cgtgcaggtg gacgagcagg
     9721 cctggcggcg catggaggac ttcatcgaga cccgcacgct ggccgagttc acgcacggcg
     9781 tctgccccga atgctaccag cgcgcgctgg agggcttgtc cggccccccg cccggcgccc
     9841 cgccgccgga gtcgacgctt gaccgcgggc cctcgagccc ctagactgtc cgcgcttttc
     9901 atgttcccgg ccggaagccc tcgtggcctg accggccggt agtcgcgggg agaaccccca
     9961 gatagaagga gcaacgccgc catggtcgca aagacggaag ccaagccggt cgccgaaaat
    10021 gacggggaca agaatgaagc cgttgttccc tatgcgctgc tcttcgtcct cgaagacctg
    10081 gctgtaaacg gccggcaggt cctctccgac gcgcttcgcc gccacgccca ggagagcaag
    10141 gagaagtttg acgaccggtt gttcatgcgc cacggcctga cgggttcgcc ggcggccagc
    10201 ctgcccgcga ttttgggcgg cgacgcggaa tcgcaaaaag cggtccagca gcgcgtcctg
    10261 gacaagctgg cggccgacaa ggccgggctg aatcccgccc tggccagcgt cctgaaggcc
    10321 gccgcccagc gccgcatccc cgccctggcc ctgacgagcc tgccggaaga tgcagcccgc
    10381 gcgatcgccg ggcggctggg cctggaggag atgggcgtca cgctgttcct tgtcaaggat
    10441 gccgcggccg atttcccgac gccggatacg tgggtcaagg ccgcgcgctc gatccagcgg
    10501 cggccccgcg gttgcggcgc gctggccgcc agcgggcggg tcgcccgcgg cgcggtcatg
    10561 gccggcatga gttgcgtcgc gctgcccgac gaactcaccg tcttcgagga cttcggcgga
    10621 accgccctgg tgatcgagac gcccggcgac gcctcgccct ccgaaatcat cggctccctg
    10681 ttccgccaga tatgatgtaa ccctttcccg ccatttatat tatggttcag gccaaagatc
    10741 cgggcggaaa gccgatcgac cggctgcgcg aagtgatgcg catgcttcgc gcggagaacg
    10801 gctgtccatg ggaccgcgaa cagacgctcc agacgctcaa gcctttcctg ctcgaggagt
    10861 gctacgaggc catggacgcc ctcgaaagcg gcgaccgcgc ccggctctgc gacgaactcg
    10921 gggatgtcct gctccaggtg gtgttccagg cgcaggtggc cagcgaggag gggagcttca
    10981 ccttcgacga cgtggccacg gcgatctccg acaagctggt ccgccgccac ccgcatgtct
    11041 tcggcgaggt ccaggtcagc gactcggccg aggtgatccg gaactgggag gccatcaaga
    11101 agggcgagaa ggggccgccc cgcaagtccg ccgtcgacgg cgtgccgcgg agcgcgcccg
    11161 ccctgcataa ggcccagcag atccagaagc gggccgcgcg cgtcgggttc gactgggaca
    11221 cggtccacca ggtgatggcc aaggtggagg aggaagtagg cgaggtccgg gaggcgatgg
    11281 ccggcggcga cccgaagaag gtcaaagagg aaatcggcga cctgctgttc gccgccgtca
    11341 atttgagccg gttcctcggc cacagcgccg aggaggcgct cgaggatacc gtccggaaat
    11401 tcacccgccg attccagggc atcgaggagc ggctgcacgc ccagggccgg aagatgacgg
    11461 agtgcaagct ggccgagctc gacgccatct ggaacgaaat caaggccgcc gagtagaggc
    11521 cggggaaagg ctggtcgccc cggcggttgc ggccgcgcaa aacaaacctt gaccgcccca
    11581 ggtctctgta gtagaaagta ttgtttgcca gtacaccatg ccggtgacgc tgaagagtgg
    11641 gtccgcaccc actcttttgt gtatcgcgct tggtggtagt gggttgcgta agcggccccc
    11701 aaagcggggt ggtggtcatg aacaacgacc tgttggctgt catagattac atggagcggg
    11761 agaggggcat cgatcgcgag acccttctta cggccgtgga agccgccctg ttgagcgcct
    11821 cgaagaagag cgtgggtccg tccaaggacc tgcgcattga gatcgaccgc aaaacctgcc
    11881 ggatccgggc tttggccaag gtgaccgtcg cggagaaggt caacgccccg catgacgaga
    11941 tcaccctccc cagcgcccgg cggatcaagg cggacgccca gctgggcgat accgtggaga
    12001 tcgaggtcac accgaagaat ttcggccgga tcgcggccca gaccgccaag caggccatcc
    12061 tccataaaat ccggcaggct gaaaaggagc gcgtcttcgg cgagtacaag gaccgggcgg
    12121 gggacatcgt cagcggcacg gtcagccgga tcgagcgcag cgacgtgatc ttggacctgg
    12181 ggcgcgccga gggcctgatg ccggtcgcgg agaaggtgcc cacggagcgg taccagatcg
    12241 gcgaccggtt gcgcgccctc atcctcaagg tggaaagccg gacgcagggc ccggagatca
    12301 tcctgacccg gagccacccg gatttcgtgc ggcggctgtt cgagctcgag gtttccgaga
    12361 tcgcggacaa gaccgtcgag atcaagggca tcgcccgcga ggcggggttc cgcagcaaga
    12421 tcgccgtggt ttcgcacgat cccaaggtcg acccggtggg ggcctgcgtg ggcatgcgcg
    12481 gcatccgcgt gaaaaatatc gtccgggagt tatccggcga gaagatcgac atcgtgcgct
    12541 ggcacgatga catcaagacg ttcgtgacga acgcgctggc gccggccaag ctgatccgcg
    12601 tcacggtgga cacggaagcg caatcggtcc aggtggtggt ggatgcggag cacctgtcgc
    12661 aggcgatcgg gaagaagggc cagaacgccc gcctgacgtc caagctgacg ggctggaaga
    12721 tcgacatcct gcgggacgag agcgacgtca gcttcgagga gaaggtggcg cgcgccgtgg
    12781 ccgcgctggc cgtcgtggac ggcatcggcc ccgagcgggc ggagaaactg gtgcatgcgg
    12841 gattcctgac gctggaaggc ctgctggcgg ccgagcccgc ggacctggaa gcggtggaag
    12901 gcattgatgc cgcggcggcc cgcgaggtgc tgcagggcgt cgagaaggcc tgcgaacacc
    12961 tgcacggaca agaggaatct catgagagtt catgaactgg ccaaggaact ggggctgacc
    13021 agcaaggagc tgatggaccg gctccacgcg atgagcgtgg aggtccggaa caatttcgcc
    13081 gccctgtcgc ccgagcaggt ggccctggtg cgcaaggccg tcggcgctcc ggccgccgcc
    13141 gcggcgcccg cggaaaccgg cgccgcaaaa ccggcgccca aggccgcgcc gccggcgaag
    13201 gccgcccccg cgccggccaa gcccgcggcg cctccgcccc ctgcgccggc cgccccgcct
    13261 ccgcctcccc cgccgcccgc gcccaaggtg atcatcgtca aggggccgat catcgtgaag
    13321 gagttcgccg agcagctggg cctgaagccc aacctgctga tcgccgagct catgacgatg
    13381 aacgtgtttg cctcgatcaa cgagcgcatc gagttcaagg tggcccagca gctggcctcc
    13441 cgccacgggg cctcgatcga gcacgagaag aaggcccccg agccgaagcc ggtcaagaag
    13501 gtcgagccga aggtcgagga gccgcgcccc gaggacatcc agccgcgccc gccggtcgtc
    13561 acgttcctcg gccacgtgga ccatgggaag acgtccctgc tggaccgcat ccgccacagc
    13621 aaggtcgtgc agtccgagga cggcggcatc acccagcaca tcggcgcgta cacggtgacc
    13681 taccgcgacc acccgatcac gttcctggat acgccgggcc acgcggcctt cacggccatg
    13741 cgcgcccgcg gcgcgaacct gacggatatc gccgtgctga tcgtcgccgc ggacgacggc
    13801 gtgatgccgc agacgaagga agccatccag cacgcccggg cggccaacgt gtgcatcatg
    13861 gtcgccatca acaagataga cttgaagagc gcggtcgtgg accgcgtgaa gaggcagctc
    13921 cagcacgagg gcctggcccc cgaggactgg ggtggccaga ccatctgctg cccggtcagc
    13981 gcggtcaccg gcgacggcat cgaccacctg ctggagatga tcctgctgca gtccgagatg
    14041 ctggagctca agggcaatgc gcggcggccg gcgcagggct tcgtgatcga ggcgcgcctc
    14101 gaggcgggca gcgggccgac ggccaacctg ctggtgaagc aggggaccct ctcggtgggc
    14161 gacgccatcg tgtgcggccg ctgctggggg cgggtcaagg cgctgatcaa cgaccagggc
    14221 atcaaggtgc gcacggccgg gccttccatc cccgtgaagt gcctgggcct gaacggcgtg
    14281 cccgagcccg gcatggagtt cgtggtatat cccaacgacc gcgaggcgcg cgcgatcgcg
    14341 gaagagcggg aggcggccag ccggctccag gcgatttccg cgccgcgccg ggcgtccctc
    14401 gacgacctgc tcaagccggc cgagacacag cagcgcacgg agctggccat cgtgctcaag
    14461 gcggacgtgc aggggtcgct cgaggccatc cggcaggcgc tgtcgggaat caagagcgac
    14521 aaggtcttgc tgcggatcgc cctggcgggc gtcggcaaca tcaccggcaa cgacgtgctg
    14581 ctggccagcg cgtccaacgc gatcgtcgtc gggttccacg tcagcaagga ggacggcgtc
    14641 gccgccctgg ccaagcgcga gggcgtggag atccgcctct acagcgtgat ttaccagttg
    14701 ctggacgagg tccgcgacgc gatggtgggc ctgctggagc cggtgatcaa ggagaccgtg
    14761 atcgggcacg cggaaatccg acaggtcttc gagatcggca agaagggcaa cgtggccggc
    14821 tgcatggtga cggacggccg cataacgagc cacgcgcgcg cccgcgtgaa gcgggcggac
    14881 gacgtgctct acgagggcgc ggtggtctcc ctgaagcgct tccaggacga cgcctccgag
    14941 gtccgcgagg gcctggagtg cggtatccgg ctcgaccgtt tcaccgattt caagccgggc
    15001 gacgtcatcg agtgttacga agttcagaag atcgagcagc cgctttaaac cgaggccgtc
    15061 gtttctgccg cggcgcctgc atcccggagt cggtcatgag tcatcagcgc atggtcaggg
    15121 tgaacgaact cctcaagcgc gagatcgcgg acgcgctttt ccgggtgatg aacgagtcgg
    15181 ggttcgacct gacggccgtg acggtcacgg gggtcgaggt cggcagcgac ttgaggacgg
    15241 cggtcgtgcg cgtgtccatc cgggaccacc gggcggagcg gggcgacatg ctcaagcggc
    15301 tgtaccggca ccggggcgag atccaggaga tcctgcaccg ccacgtcatc ctgaaataca
    15361 cgccgcggct ttccttcgag ctggacgaat ccatcgagaa gggcgatcgc gtcctgcata
    15421 tcctgcacaa gatggagaat gccggcgaac tgggcgcggg cggcgcgggc gggagcccgg
    15481 cgacatgagc gggggccgct atgccgcccc gcctcccgtc atcgagcctt gggagggcct
    15541 gctgctggtc gacaagcccg cgggcatgac gtcccacgac gtcgtcgacg ccatccgccg
    15601 ccggttcggg ttccggaagg tcggccacgg cggcacgctt gacccgatgg cgaccgggct
    15661 gctcgtgatc ctgctcgggc gcggcaccaa gctgtccgac cgcgtcatgg cctccgacaa
    15721 gacgtacgag gggaccttga agctcggcgc gacgacggac accgaggacg tggacgggca
    15781 gatcctggcc gaggccccct gggagcacgt tacgcgggag gcggtggagc gggaaatggc
    15841 cgcccgggtg ggcgacctca tgcagacgcc gcccatggtc tccgccgtaa agaagcaggg
    15901 ggtcccgctg tacaagctcg cgcgcaaggg ccagaccgtc gagcgggagc cccggctgat
    15961 ccatgtctac gagttccggc tcctggcctt cgagccgccc cgggcccggt tcgtcctgcg
    16021 ctgcaccaag ggcacctatg tccggacgct ctgcgccgac atcggccggg cgctcggctg
    16081 cggggccttc ctggaggcgc tgcgccggac gcgggccggc gccctggccg tggaggccgc
    16141 cgcgccgctg gacgacatcc tgcgcatgga cgtggaccgc gtcgcggagc gggtccttcc
    16201 cctgcacgga ttcctcgcgg gagggtcgcc cggatgaagc tgcgtcgggc cctggaggag
    16261 atgtcccgag agcccggccc cagcaccgtg gccatcggga cctttgacgg ggtccaccgc
    16321 ggc
//
LOCUS       DAOOTK010000067        16153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2461_length_16153_cov_6.863275, whole genome shotgun sequence.
ACCESSION   DAOOTK010000067 DAOOTK010000000
VERSION     DAOOTK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_16153_cov_6.863275"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /gene="murF"
                     /locus_tag="PK634_08640"
     CDS             <1..1189
                     /gene="murF"
                     /locus_tag="PK634_08640"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOW97841.1"
                     /translation="AETCPAAGTAGPVLVVGDTRKALGDLASGYRRTVTARLVGVTGS
                     VGKTTVKEMIADALAQAAPTARTPGNWNNDIGLPLSLLAIEPVHRYAVLEAGTNHPGE
                     IAALCRGMRPDWGVVTAIGQGHLEHFGSVEAVAREKADLLRALPAGGLAFLPRDDAWY
                     ELLRAAAPCPVVTVSLEAGADYRGRPDPAGGDRFVVEERETGERAELVSPLPGRHMVA
                     NALFAAAVGRRCGLPWAAISAALARYRPPPLRWNRSVVRNVTFINDAYNANPMSMRAA
                     LEAFGRMAVAGRRWLVLGGMLELGPGECDLHRQLGRVVAGGPWEGLLAVGPRGAWIVE
                     GTREAGLSADRSFCCADAGAAARRLDQWARPGDAVLLKASRTERLEKVLESFAAAPGA
                     GRE"
     gene            1200..2339
                     /gene="mraY"
                     /locus_tag="PK634_08645"
     CDS             1200..2339
                     /gene="mraY"
                     /locus_tag="PK634_08645"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOW97842.1"
                     /translation="MFYYLHLLTDWFSPLRVFRYITVRAIAGAGTAFLFSLLVGPWLI
                     RELRRFKMGQQVRKDEAPPLYVFHGKKAGTPCMGGLLIIASVLVSTLLWAVPTNEFVL
                     LTLATMCYMGLVGFRDDYLKVTRKSTRGLGVRAKLIYQLGWVAVVVTILLWLPSTREH
                     VQQFLVPFVKDPVIRHMGFLAIFLFLALVMVGSTNAVNLTDGLDGLAIGCSNSVAVSY
                     LVMAYVAGHARFAQYLQVPYIAGSGELAVFCACLLGAGLGFLWFNCHPARVFMGDTGS
                     LALGGAISMVAILIKQELALVIVGGVFVIEAASVLLQVSFFKLTGGRRIFKCAPLHHH
                     FEVQEKERAEQEHRDVEVIETMITTRFWILSIIFALIGIATLKIR"
     gene            2345..3673
                     /gene="murD"
                     /locus_tag="PK634_08650"
     CDS             2345..3673
                     /gene="murD"
                     /locus_tag="PK634_08650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOW97843.1"
                     /translation="MSKRALVLGLGESGEAAARLLRAEGAAVMAADGVDTPALREKAG
                     RLSAEGIEVALGANPLPCGAFDLAVVSPGLPPASPWLQEVKRRGIPAISELELGWSRR
                     SCRVVAVTGSNGKSTAVKWLAEALQQAGLRAMPAGNYGAAICRVVREQPGLDWLVLEV
                     SSFQLETVDAFRPEVGVLLNILPNHLDRHGSMESYAALKARLFARTLPSDTCLVPAAR
                     RDEVRALSGGAGRWLAFGAGPESDYRWREGRVWRGGAACADLRGTRFDNDVLGPAAAA
                     VVAAAEACGADAACAERAARDFQPLPHRMQKVAEGWGVWFVDDSKATNVAAMVAALRM
                     AGRPVRLVAGGLAKETDFRPAREVLREFARGVYLVGHAAEAMRRAWSDAVPCVLCGTV
                     DEAVRRAAKEAAAGETVLLSPACTSYDQYKNYGERGEHFARCARAVAEGA"
     gene            3740..4648
                     /locus_tag="PK634_08655"
     CDS             3740..4648
                     /locus_tag="PK634_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOW97844.1"
                     /translation="MKSSVLIAAVVGIHVLAVGGVAIMQGCETRRVVTEQPPPPAPPM
                     PPKAETLPAPVPKPVFQPPVPVEPAPSVMEPGAGRTYEIQNGDSLSKIAARFGVSTRE
                     LADLNKIKDPNNIRIGQKLVLPDYAKDQPASSSKPKAKAKVAVPEGAETYTVQAGDML
                     SKIAVKYGVKTADLRAANNLSGDKIIVGQKLVIPGAKAEKKEQPKDAAPKAEEAAPLA
                     EPPPAPATEPVMAPPAPVTEPAASAAPQLQGEEPPMDYTVQPGDTVDSIAKMYIIRKE
                     QILQLNNLTEGAELKPGQKIKLPTTM"
     gene            4756..5883
                     /gene="ftsW"
                     /locus_tag="PK634_08660"
     CDS             4756..5883
                     /gene="ftsW"
                     /locus_tag="PK634_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOW97845.1"
                     /translation="MVILIGIVLILVTVGIVMLASTSSVQGGTQYHDPLFFVKRQAVG
                     LVVGAFIALFAARLDYHYWRPLAIPLALASLVLLVLVLIPGVGQTVKGSSRWLRMGPA
                     TFQPSELAKFSSVILISWWMSRFQLHARELRMGLVYPLALLGLFLGLVFIEPDFGTTM
                     LLAMVGMLLLFLGGSRVGYLAVAGVGGATLFSLAILHDPVRLRRITAFLDPEQYARKE
                     AFQLLNAIYAFVVGGGRGVGFGQSLQKHFYLPEAHTDFIFAIIGEELGMKGSLGVLAL
                     FLGLFLCGLYISYRAPDRFGQLLGCGCTLMLSLQALINMGVVTGSLPTKGLPLPFISF
                     GGSSLVASMALVGVLLNLARQAISEEDERDARCIRDAARRI"
     gene            complement(5888..6658)
                     /locus_tag="PK634_08665"
     CDS             complement(5888..6658)
                     /locus_tag="PK634_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97846.1"
                     /translation="MTDSTALYRFLKWTWLLGQCLMPVLAAVYLSRHPGLLAAPPGLT
                     SPFATALYRLLLHVSASAYLILWLIAEAMLCMYRFPLRTAGAAGLVGGFLLQFAVLAV
                     TGQSACFAAFTGLYVTLAALFACLVWLGLAAATRQGSGRGRPGGLAAWAPAVALLALG
                     ILPLAFLFRPWWAAFAGLRPAFRAVNGLILLLNAAATARTLAGFSIWGRTDPQETLFY
                     REWEKWAAPTIILLILTVVGAILVAGISGAVSGGGPAE"
     gene            complement(7096..7305)
                     /locus_tag="PK634_08670"
     CDS             complement(7096..7305)
                     /locus_tag="PK634_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016749018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOW97847.1"
                     /translation="MDRIPVQSTNVAEVGYDPATMTLEVAFHNGTLYQYFDVPEALYQ
                     EMLRSDSVGKFLNTQIKNSYRYAKL"
     gene            complement(7314..9155)
                     /locus_tag="PK634_08675"
     CDS             complement(7314..9155)
                     /locus_tag="PK634_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW97848.1"
                     /translation="MSNSPTYLGTVQDVQGATISVALDTDTVSGLAFIEGHGYRIGQI
                     GSFVRIPMGFTDLFGIVSQVGAGAVPEALAKVEPYGHRWLKVQLIGEGQRAGEFRRGI
                     SQYPTIGDEAHLVTEQDLSRIYGRPDAPNFVRVGSLASAESIPSLIDIDRLVSRHSAV
                     VGTTGAGKSTTVANLLVSVSDPTRYPSARIIILDIHGEYYTALHDRATVFRVNADEKQ
                     GEKPLFVPYWALNFDELLRVTPFRSLTDADRAALVEKVKQLKLLSLQSQARNGVTPDT
                     MTVDTPIPFSIHRLWYELHRLVCSTHNQPGANQTDASEAIEVDGAGQKLLGDIMRVIA
                     PKYRPITQGGANRVYLSGSPLNIRRQILALESFLRDTRYDFLFRPGPWCPDPDGKPAQ
                     DLDALLTSWIGGDKPITILDLSGVPVSILMDLVGVLIRLLFDALFWARFLPEGGRSRP
                     LLFVLEEAHAYLNAGNEGAASIAARRIVKEGRKYGIGAMIVSQRPAEIDPTILSQCGT
                     LFAMRLANTSDRSHVTGTVSDNLEGLFNMLPSLRTGEAIIVGEAVQLPLRTLIDAPVK
                     NRRPDSHDPLVYDPNNKGGWNSAKQAGDYAKVLELWRSENPRANQKA"
     gene            complement(9145..10356)
                     /locus_tag="PK634_08680"
     CDS             complement(9145..10356)
                     /locus_tag="PK634_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOW97849.1"
                     /translation="MNQAHDPFRQVNYLQQCLSNDKKPLGLFLGAGCPVAVKSGDDKP
                     LIPDIAGITAEVRERLAKCKDCGPLLKAVEGHFVTDERKETNVEDMLSHIRALRIVAG
                     KEKARGLSAADLDRLDSDICKIIHELADKALPGTGTPYHRAALWTDAVGREHPVEVFT
                     TNYDLLLEQAFEDCRVPYFDGFAGSRRPCFDIRAMEEDKLPPRWARIWKLHGSINWYQ
                     DPARGVLRGATNETGLKRVIHPSHLKYEESRRMPYLAMMDRLRAFLKQPSSALVISGY
                     SFRDEHINEVIVQGLQATQTAVAFALLFEDLSKYTKAVKLASERTNLTLLARDGAVVS
                     GRESKWPEREADSAAADAVPWVSWVPVNPKEENGRLKAVFHLGDYAVLGDFLHALVGH
                     IRQAQEAPNVK"
     gene            complement(10353..11459)
                     /locus_tag="PK634_08685"
     CDS             complement(10353..11459)
                     /locus_tag="PK634_08685"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOW97850.1"
                     /translation="MKRWPIKRLGELMLPTEQRDPRDSPSNEFLYVDIASVDNRAKSI
                     VTAQTVIGADAPSRARKVIRTGDVIVSTVRPNLNAVALVPHNLDDQICSTGFSVLRPS
                     PEVLSGYLFAFVRSPSFVKYLVARTTGANYPAVSDGQVKDVPIPVPPRGEQERIVRLL
                     DEADALRRLRTRADQRTANLIPALFHNLFGNPALNPKGFRKEPLGKLLHVKSGESLPA
                     KEMTTNGSFVVYGGNGITGHHDKFMFENPVIVIGRVGVYCGAIHLSEPKSWVTDNALY
                     VSEMKEDFAQRYLLTALRVANLNQYAGRAAQPLVSGSRIYPVPILIPPLPLQQEFADR
                     VRDIDELESVQSRSTARLNDLSQSLLHSAFGGAS"
     gene            complement(11456..12949)
                     /locus_tag="PK634_08690"
     CDS             complement(11456..12949)
                     /locus_tag="PK634_08690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOW97851.1"
                     /translation="MNGNGNSFQSSGLRQKVDALMDILWAGGLNNPMDAIEQMSYLIF
                     LRLLSEKDDLLAKLDKKYKRIFTDKWARYAWGNFVTLTGDQLFDSIRAAIESIHELPG
                     MSATGKLLFNRATLKIYDRPTLRAVIQGIHEMSFDTGNGDDPKGDMYEYLLSKLSTSG
                     TNGQFRTPRHIIDMIVRLVDPKPDMRICDPACGTAGFLISAYNHILRQNTSKPELEKG
                     HVSGDLLKAAQWRFLEEQAFTGYDNDANMVKIAILNLYLHKLEKAGIEFHNPLTTNKT
                     GQYPGMTYDVVLANPPFSGKVQQESILSNLNHGLNTRATELLFVKWFVDHLAPGGRAG
                     VIVPNGVLFGSNKAARRVREMLLHDCDLQAVVSLPSGVFRPYAGVATAALIFQKGTRT
                     KEVWFYDLTADGFSLDDKRTPIDANDIPDVLSKWPGHEEGPNSYRVSLERIEENDLTL
                     AAGRYRPVTQQAVNHDSPADILADILKIEGEISKRATDLLSEVRHKP"
     gene            complement(12946..15282)
                     /locus_tag="PK634_08695"
     CDS             complement(12946..15282)
                     /locus_tag="PK634_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOW97852.1"
                     /translation="MLDPQAVRFELRGNDGRADYLLLGMRGPLCVLEAKREDLDPYEA
                     KEQGRGYAENVRAPFVILSNGREHWFWNLQRGDADAFRIERLPSPKDLERLLLKNLQP
                     PRPLLSEKVGPDYLRAFKPDVIVRGYQIKSIDEVARQFDQQRKRKFLLEMATGTGKTL
                     LCAALIRRFLTTRNAERVLFIVDRIELAKQTMEDFNVILREFNPVIFKTARRKPAELL
                     GSCVVVATLQSLMVDRRYREEFTPFHFDLVINDEAHRSIYGDAREVVQFFQATRIGLT
                     ATPKAYLKNINAEQLSQENPKALEARILRDTYHYFGCEPGIPTFRYDIIEAVKDPEGP
                     FLCMPKIFDIRSDITTKALEESGWTVLISEQEENFKVQDLERKIFTPQRNRVMCEAFL
                     KHVQKDPAGKLGKSIIFAVNQDHATNLTKILNALEPGIAMTITSRIPVSSDLAKDFRD
                     GKRAERVAVSVDMLSTGYNCRDLLNVVLMRPIFSPTEYIQIKGRGTRRFTFRVGNTEY
                     EKKHYFLLDFCGVAEYFEEEYDYSLPLALPRARGEQAQAEATGEKKPAFVAEAGGKYE
                     AKPGAEEPEKREIPVWEGRDVIVSEEIRIVGPNGEKVDVMTFRGSFEKDVKVFAGKDR
                     EFQEAVEVEDDDTVETILQERFFHKPEMFYSPDKLIKSYGVPAPTPAFVYSALGKKPL
                     PTKDAIVTDTVDSIAARFNLRYSEAKWLSATAQLIADDPQNLRRFVQGDMTVFDSSQF
                     SHLGGLGQLARFEHRDEAFEALRQSTLVRQSLLGIEAA"
     gene            15837..>16153
                     /locus_tag="PK634_08700"
     CDS             15837..>16153
                     /locus_tag="PK634_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97853.1"
                     /translation="MAGVGINERSETAFDEVFRHWKKMTPKIRTLENATAAKARKAPA
                     FSRSQAPHEASCAVVQTARLALFSWLVVAAQGGIGVVGEWSPSVRSLFLFARAPENED
                     EKTG"
BASE COUNT         2951 a         4967 c         5140 g         3095 t
ORIGIN      
        1 ggcggaaacc tgccccgcgg cggggaccgc gggccccgtc ctcgtcgtgg gcgacacgag
       61 gaaagcgctg ggcgacctgg cttccggcta ccgccggacg gtcacggccc ggctggtggg
      121 cgtcacgggc agtgtgggca agaccacggt gaaggaaatg atcgccgacg cgctggcgca
      181 ggccgccccg accgcgcgca cgcccggcaa ctggaacaac gacatcgggc tcccgctaag
      241 cctgctcgcg atcgagccgg tccatcgcta cgccgttctc gaggcgggca ccaaccaccc
      301 cggcgaaatc gccgcgctct gccgcggcat gcggccggac tggggggtgg tcacggcgat
      361 cgggcagggg cacctcgagc acttcggatc cgtcgaggcg gtcgcccggg aaaaagccga
      421 cctgcttcgc gcgctgcccg ccggcgggct ggcgttcctg ccccgggatg acgcgtggta
      481 cgagctgttg cgggccgcgg cgccctgccc ggtcgtaacg gtctcgctgg aggccggcgc
      541 ggactaccgg ggccggcccg atccggcggg gggggatcgc ttcgtcgtcg aggagcggga
      601 gacgggcgag cgcgccgaac tggtttcgcc cctcccgggc cgccacatgg tcgccaacgc
      661 cctcttcgcc gcggccgtcg gccggcgctg cgggcttccg tgggcggcga tctcggccgc
      721 cctcgcgcga taccgcccgc cgccgctccg ctggaaccgc tccgtcgttc gaaacgtcac
      781 gttcatcaac gacgcgtaca acgccaaccc gatgagcatg cgggccgccc tggaggcctt
      841 cggccggatg gcggtcgcgg gccgccgctg gctggtgctg ggcggcatgc ttgaactcgg
      901 gcccggcgag tgcgatcttc accggcagct gggccgcgtc gtggctggcg ggccgtggga
      961 ggggctgctg gccgtcgggc cgcgcggggc gtggatcgtc gaaggaaccc gggaggccgg
     1021 actctcggct gaccggagtt tctgctgcgc ggatgccggg gccgccgcgc ggcggctgga
     1081 ccagtgggcc cggccgggcg acgcggtgtt gctcaaggct tcgcgcaccg agcggttgga
     1141 aaaagtcctg gagtcttttg ccgccgcccc gggcgccggg cgggaataaa ggatcgatca
     1201 tgttctacta cctgcacctg ctgaccgact ggttctcgcc gctgcgtgtt ttccgctaca
     1261 tcaccgtccg ggcgatcgcc ggcgcgggca cggcgttcct gttcagcctg ctggtcggcc
     1321 cgtggctgat ccgcgaactg cgccgcttca agatgggcca gcaggtgcgc aaggacgaag
     1381 cccctccgct ctacgtcttc cacggcaaga aggccggcac cccctgcatg ggcggcctgc
     1441 tgatcatcgc gtccgtgctg gtctccaccc tgctgtgggc ggtgccgacc aacgagttcg
     1501 tcctgctgac cctcgcgacg atgtgctata tgggcctcgt gggattccgg gacgactacc
     1561 tgaaggtcac gcgaaaaagc acgcgcggcc tcggcgtgcg cgcgaagctg atctaccagc
     1621 tgggctgggt ggcggtcgtc gtcacgattc tgctctggct gccgtccacg cgggagcacg
     1681 tccagcaatt cctggtcccc ttcgtcaagg atcccgtgat ccggcacatg ggcttcctgg
     1741 cgatcttcct gttcctggcc ctcgtcatgg tcggttccac caacgcggtg aacctgaccg
     1801 acggcctcga cgggctggcg atcggctgca gcaattccgt ggcggtgtcg tacctcgtca
     1861 tggcctacgt cgcgggacac gcgcggttcg cacagtacct gcaggtcccg tacatcgccg
     1921 gcagcgggga gctggccgtg ttctgcgcct gcctcctcgg cgccgggctg ggcttcctct
     1981 ggttcaactg ccacccggct cgcgtgttca tgggcgacac gggcagcctt gcgctcgggg
     2041 gcgcgatctc catggtggcg atcctcatca agcaggaact ggccctcgtc atcgtcggcg
     2101 gggtgttcgt gatcgaggcc gccagcgtcc tgctccaggt ctcctttttc aagctgacgg
     2161 gcggccggcg catcttcaag tgcgccccgc tgcaccatca tttcgaggtg caggagaagg
     2221 agcgggcgga gcaggagcac cgggatgtcg aggtgatcga gacgatgatc accacccggt
     2281 tctggatcct ctcgatcatc ttcgccctga tcggcatcgc caccctgaaa atccgatgac
     2341 gcctgtgtcg aaaagagcac tggtgctggg cctcggcgag agcggcgagg ccgccgcccg
     2401 cttgctgcgc gcggaaggcg cggcggtgat ggcggcggat ggcgtcgata cgccggcgct
     2461 gcgggaaaag gccgggcggc tctccgccga ggggatcgag gtcgccctgg gcgcgaatcc
     2521 gctgccgtgc ggggccttcg acctggccgt ggtcagcccg ggccttcccc cggcctcgcc
     2581 ctggctgcag gaggtgaagc gccgcggcat ccccgcgatc tccgaactgg agctgggctg
     2641 gagccgccgc tcgtgccgcg tcgtggccgt gaccggctcc aacggcaaga gcacggcggt
     2701 caagtggctg gcggaagcgc tccagcaggc gggcctgcgc gcgatgcccg ccgggaacta
     2761 cggcgccgcg atctgccgcg tggtccggga acagccgggc ctcgactggc tggtgctgga
     2821 ggtcagctcg tttcaactcg agacggtgga cgcgttccgt cccgaggtcg gggtgctgct
     2881 gaacatcctg ccgaaccacc tggaccggca cggcagcatg gaatcgtacg ccgcgctgaa
     2941 ggcgcgcctg ttcgcccgga cgcttccgtc cgatacctgc ctcgtgcccg ccgcgcggcg
     3001 cgacgaggtg cgcgcgctgt ccggcggagc cggccgctgg ctcgcgttcg gagccgggcc
     3061 ggaatcggac tatcgctggc gcgaggggcg ggtctggcgc gggggcgcgg cgtgcgcgga
     3121 cctgcgcgga acccggttcg acaacgacgt cctgggcccc gccgccgccg cggtggtggc
     3181 cgcggccgag gcctgcggcg ccgacgccgc gtgcgccgag cgggccgcgc gcgacttcca
     3241 gccgctgccg caccgcatgc agaaggtggc cgaggggtgg ggcgtatggt tcgttgacga
     3301 ttccaaggcc accaacgtcg cggccatggt ggccgccctc cggatggccg ggcggcccgt
     3361 ccgcctggtc gccggggggc tggccaagga gaccgatttc aggcccgccc gcgaagtcct
     3421 gcgcgaattc gctcggggcg tgtacctggt cggccacgcg gccgaagcca tgcggcgggc
     3481 ctggtcggat gccgtgccgt gcgtcctctg cggcacggtg gacgaagccg tgcggcgcgc
     3541 ggcaaaggag gcggccgccg gggagacggt gctgctctcg ccggcctgca ccagttacga
     3601 ccagtacaag aactacgggg agcgcggaga gcatttcgcc cggtgcgccc gggccgtcgc
     3661 ggagggcgca tagactgttt gcattgcgga tggaacaggc tctatactac ggagcgttaa
     3721 taccaacagg agaaatggaa tgaagtcatc ggtgttgatt gcggcggtcg tcggcatcca
     3781 cgtgctggcg gtcggtggcg tggcgatcat gcaggggtgc gagacccgcc gggttgtgac
     3841 ggagcagcct ccgccgccgg cgccgcccat gccgccgaag gcggagaccc tgccggcgcc
     3901 ggtgccgaag ccggtcttcc agccgcccgt gccggtggaa cccgctccct ccgtgatgga
     3961 gccgggcgcc gggcgcacct acgaaatcca gaacggggac tcgctctcga agatcgccgc
     4021 gcggttcggg gtcagcaccc gcgaactggc ggacctgaac aagatcaagg acccgaacaa
     4081 tattcgcatc gggcagaagc tggtgttgcc ggactacgcc aaggatcagc cggcctcctc
     4141 gtccaagccc aaggccaagg ccaaggtcgc cgtgcccgaa ggggcggaga cctacacggt
     4201 gcaggccggg gatatgctct cgaagatcgc cgtcaagtac ggcgtgaaga cggctgacct
     4261 gcgcgcggcc aacaacctgt ccggcgacaa gatcatcgtg ggccagaagc tggtgatccc
     4321 gggcgccaag gcggagaaga aggagcagcc caaggacgcg gcgcccaagg ccgaagaggc
     4381 ggcgcccctc gcggaaccgc cgcccgcgcc ggccacggag cccgtgatgg cgccgcccgc
     4441 gccggtcacc gagcccgccg cgtccgccgc cccacaactg cagggcgagg agcccccgat
     4501 ggactatacc gtccagccgg gcgacacggt ggattcgatc gcgaagatgt acatcatccg
     4561 caaggagcag atcctccagt tgaacaacct gacggagggc gcggaactca agccgggaca
     4621 gaagatcaag ctcccgacga cgatgtaacc agcgtcgagc gcggccgcgg ggtcgcggga
     4681 agggcccggg gacacccgtt gcaggggttg cccgggcttt tctgttgagg cgggcgaacg
     4741 gtcatgtaca ggaccatggt catactcatc gggatcgtgc tgatcctggt gacggtcggg
     4801 atcgtcatgc tggccagcac gagttccgtg cagggcggca cgcagtacca cgatccgctc
     4861 ttcttcgtga agcggcaggc ggtggggctc gtggtgggcg cgttcatcgc cctgttcgcc
     4921 gcccggctgg actaccacta ctggcggccg ctggccatcc cgctggcgct cgcctcgctc
     4981 gtcctgctgg tcctcgtgct cataccgggc gtggggcaga cggtgaaggg cagcagccgc
     5041 tggttgagga tgggaccggc gacctttcag ccctcggagc tggcgaagtt ctccagcgta
     5101 atcctgatct cctggtggat gtcccgcttc cagcttcatg cgcgcgagct ccggatgggc
     5161 ctggtctatc cgttggcgct gctgggcctc ttcctgggac tggtgttcat cgagcccgat
     5221 ttcggcacga ccatgctgct ggcgatggtc ggcatgctgc tgctgttcct gggcggcagc
     5281 cgggtgggct acctggccgt cgcgggcgtg ggcggggcga cgctgttcag cctggccatc
     5341 ctgcacgatc ccgtgcggct ccgccgcatc acggccttcc tggatccgga gcagtatgcc
     5401 cgcaaggagg cgttccagct cctcaacgcc atctacgcct tcgtcgtggg cggcgggcgg
     5461 ggcgtcgggt tcggccagag cctccagaag catttctacc tgccggaggc gcacaccgat
     5521 ttcatcttcg ccatcatcgg cgaggagctg gggatgaagg gttcgctcgg cgtgctggcg
     5581 ctcttcctgg gcctgttcct gtgcggcctc tacatcagct accgcgcccc cgatcgcttc
     5641 ggccagttgc tgggatgcgg ctgcacgctc atgctgtcgc tgcaggcgct catcaacatg
     5701 ggcgtagtga cgggcagcct cccgacgaag gggctcccgc ttccgttcat cagtttcgga
     5761 gggtccagcc tcgtggcctc catggcgctg gtcggcgtgc tgctcaacct ggcgcgccag
     5821 gccatctcgg aggaggacga acgcgacgcc cgctgcatcc gcgacgcggc gcgccggatc
     5881 tagcttgtta ttccgccggc ccgccgccgg agacggcgcc gctgataccc gccacgagga
     5941 tggcgcccac gaccgtgagg atcagcagga tgatggtcgg cgccgcccac ttttcccact
     6001 cccggtagaa cagcgtttcc tgcggatcgg tccggcccca gatcgaaaag ccggccaggg
     6061 tgcgcgccgt ggccgccgcg ttgagcaaca ggatcaaccc gttgacggcg cggaaggccg
     6121 ggcggaggcc ggcgaacgcc gcccaccagg gtcggaacag gaaggccagc ggcaggatgc
     6181 ccagcgcgag caacgcgacc gccggcgccc aggccgccag tccgccgggt cgcccgcgcc
     6241 cggatccctg ccgggtcgcg gcggcgaggc cgagccacac caggcaggcg aagagggcgg
     6301 ccagcgtgac gtagagcccg gtgaacgccg cgaagcaggc ggactgcccg gtcacggcaa
     6361 ggaccgcgaa ctgcagcagg aaaccgccga ccaggccggc ggccccggcc gtccgcagcg
     6421 ggaagcggta catgcacaac atcgcctccg cgatgagcca caggatcagg taggcgctgg
     6481 cggaaacgtg gaggagcagg cggtagagcg cggtcgcgaa cgggctcgtg aggccgggcg
     6541 gcgcggccag gaggccggga tgccgggaca ggtagaccgc cgccaggacc ggcatcaggc
     6601 actggcccag cagccaggtc cacttgagaa accgatagag ggcggttgaa tcagtcatgg
     6661 gctgttggag ccggttagcg gtagctatcc gaactcattt cgatgatgaa tatgccaaac
     6721 gtcgcgggaa gtgaaagccc gtaatctacc cgtctgtacc tcgcggcgct gtcgcgccgc
     6781 tcggaacctt cccccgcctc gtcgctgccg ctcctcgccc ccacgcacgg cgcgttgcgc
     6841 cgtgcttcta ctcctcgccg ctggacgcgg ctcggagcaa ggacggcgct gggccgcgcc
     6901 ggttgatgct cggcacgctg gccgcgtgcc tgcgcttccg ccgcccgcgt gggcgcggtc
     6961 gtcggtcgcc cctcctgaac gtcggggcga ggatggctgc cccggtcgcc gctcacgcga
     7021 cgacggcggc tctcgccgcc cggttcgttc cgcccgtctt ctcgtcttcg ttttcggggg
     7081 cgcgcgcgtc ttctcctaca gcttcgcgta gcgatagctg ttcttgatct gtgtattcaa
     7141 aaacttgcct accgagtcag agcggagcat ttcctgatag agagcttccg gcacatcgaa
     7201 atactggtac agagtgccgt tatgaaacgc gacctcaagc gtcatggtgg cgggatcgta
     7261 tccgacttcc gcaacgtttg ttgattgaac gggtattctg tccatgttca gcctcacgct
     7321 ttctgattgg cgcggggatt ttcgcttcgc cacaattcaa ggactttcgc gtagtcccca
     7381 gcctgcttcg cgctattcca cccgcctttg ttgttgggat cataaaccaa aggatcatgg
     7441 ctgtcaggac ggcgattctt caccggggca tcaatgagtg tccgcaatgg caactgcact
     7501 gcctctccaa caatgatcgc ctcacccgtg cgcagagacg ggagcatatt gaacaggccc
     7561 tcaaggttat cgctcaccgt accggtaaca tgtgaacggt cggaagtatt cgcaaggcgc
     7621 atggcaaaca aggtgccgca ctgcgaaaga atggttgggt caatctccgc tggtcgctga
     7681 ctgacgatca ttgcgccgat tccgtacttg cggccttcct tgacgattcg ccgggccgca
     7741 atggaagcgg ccccctcgtt cccggcgttc aagtacgcat gcgcttcctc aagcacgaac
     7801 aacaaaggcc gtgaacgtcc tccctccggc agaaatcgcg cccagaacaa ggcatcgaac
     7861 aataggcgaa ttaggacgcc aacaaggtcc atgagaatcg aaaccggaac gcccgaaaga
     7921 tcaagaatcg tgatgggttt gtcgccgcca atccacgaag tgaggagggc gtcaaggtct
     7981 tgcgcgggct tcccgtccgg gtcaggacac caaggtcctg ggcgaaagag gaagtcgtag
     8041 cgcgtatcac gaaggaacga ttcgagcgcg agaatctgtc tgcgaatatt tagcggagat
     8101 ccgctaagat agacgcgatt cgccccgcct tgcgtaatcg gccggtattt cggggcaatg
     8161 acgcgcatga tgtcgccaag caatttctgc ccggccccat cgacttcaat cgcttccgat
     8221 gcatctgtct gattcgcgcc cggttgattg tgtgtcgagc aaacgagcct gtgaagttcg
     8281 taccacaacc ggtgaatgct gaatgggatt ggcgtatcaa cggtcatggt gtccggcgtg
     8341 acaccgttcc gcgcttgaga ctgtagagac agcagcttga gttgcttcac cttctccaca
     8401 agggcggcgc ggtcggcgtc cgtcagactg cgaaaggggg tgacgcgcaa aagctcatca
     8461 aaattcagcg cccagtaggg aacgaacaga ggcttctcgc cctgcttctc gtccgcgttc
     8521 acgcggaaca ccgtggcccg gtcgtgcaag gcggtgtagt actcgccgtg gatgtccaga
     8581 atgatgatcc tcgctgacgg gtaacgggtc ggatcggaga cggaaacaag cagattcgca
     8641 acggtcgttg acttgcctgc gccggttgtt ccaacaaccg cactgtgccg actcacgagc
     8701 cgatcaatgt cgatgagcga aggaattgac tccgcgctcg cgaggcttcc aacccgaacg
     8761 aagttcggag cgtcgggccg tccgtaaatc cgggacaagt cttgctcagt aacaaggtgg
     8821 gcttcatcgc cgatcgttgg atattgtgaa atcccgcgtc gaaattcccc tgcgcgttgg
     8881 ccctcgccga tcaactggac cttcagccaa cggtggccgt agggctcgac ctttgcaagc
     8941 gcttcgggaa ccgcgccggc accgacttgg gaaacgatcc caaacaaatc ggtaaagccc
     9001 atcggaatcc gtacgaaact cccgatttgg ccgattcgat agccgtgccc ttcgataaac
     9061 gccaagccgg aaacagtatc ggtgtcgagg gcaacgctga ttgttgcgcc ctgcacatct
     9121 tgaaccgtcc cgagatatgt cgggctattt gacattcggg gcctcctgcg cctggcgaat
     9181 gtgcccgaca agggcatgaa ggaaatcacc gagaaccgca taatcgccaa gatggaaaac
     9241 ggctttcagt cgtccgtttt cctctttggg attcacagga acccatgaca cccaaggaac
     9301 agcgtccgcc gcggcggagt cggcttccct ctccggccac ttgctttccc gtccactcac
     9361 aacagcgccg tctcgcgcaa gcagggtgag gttggtgcgc tccgacgcga gtttgaccgc
     9421 tttcgtgtat ttcgacaggt cttcaaaaag caatgcgaag gcaacagcgg tctgcgtggc
     9481 ttgtagcccc tgaacgatga cctcgttaat gtgttcgtcc cggaatgagt agccggagat
     9541 gacgagggcg gatgatggtt gtttcaggaa tgcccgaagc ctgtccatca tcgccaagta
     9601 cggcattcgc ctgctttcct catacttcaa gtgcgaagga tggatgacac gtttcaatcc
     9661 cgtctcgttt gtcgcgcccc ggagaacacc ccgcgcgggg tcttgatacc agttgatgga
     9721 accatgcagt ttccatatcc gcgcccagcg cggtggcaac ttgtcttcct ccattgcgcg
     9781 gatgtcaaaa catggacggc gcgaaccggc gaagccgtcg aaatacggca cacggcaatc
     9841 ctcgaacgct tgctccaaaa gaaggtcgta attcgtcgtg aagacctcaa cgggatgctc
     9901 ccgaccaacg gcatccgtcc agagcgcggc gcggtgatac ggcgttccgg tgccgggcaa
     9961 agccttgtcg gcaagttcat gaatgatctt gcagatgtcc gaatccagtc ggtccaagtc
    10021 agcggcagac aaaccgcgcg ccttttcctt gcccgcaacg atgcgaagcg cccgaatgtg
    10081 gctcagcatg tcctcaacat tcgtctcttt tctctcgtcc gtaacaaagt gcccttcaac
    10141 cgctttcagc agtggcccgc aatccttgca cttggcgagc ctctcgcgga cctctgcggt
    10201 gatccccgcg atgtcgggaa tcagcggctt gtcgtcgccg gatttgactg cgaccggaca
    10261 acccgcgccc agaaagagcc ccaacggctt cttgtcgttg gagaggcatt gctgaaggta
    10321 attgacttgg cgaaatggat cgtgggcctg attcatgaag cacctccaaa agcagaatgc
    10381 agaagcgatt gcgagagatc gttcagccgc gccgtacttc gtgactgtac agattccaac
    10441 tcgtcaatgt cccggacgcg gtcggcgaac tcttgctgaa gcggaagagg tggtatcaga
    10501 atcggaacgg gatatatccg acttccggac accagcggtt gcgcggctct cccggcatat
    10561 tggttgagat ttgcgacgcg aagcgccgtc aacaggtagc gttgcgcaaa gtcttccttc
    10621 atttcgctca catacagggc attatcagtg acccacgatt tgggctcaga aaggtgaatc
    10681 gctccacagt aaacgccgac tcgaccaatc acaatgaccg ggttttcaaa catgaactta
    10741 tcgtgatgcc cagtaattcc gttccctcca taaaccacaa agctaccatt cgtcgtcatt
    10801 tctttggcgg ggagcgattc tccgctcttc acatggagca gtttgcccag tggctctttg
    10861 cggaagccct tgggattcag ggcagggttt ccaaacaaat tgtgaaaaag agcaggaata
    10921 agattcgcag ttcgttggtc tgctcgggta cggagtctcc gcaatgcgtc cgcttcgtcg
    10981 agtagcctca caatccgctc ttgctctccc cgtggcggca caggaatagg cacatctttt
    11041 acctgtccat cacttacagc gggatagttt gcgcctgttg ttctcgcaac gaggtattta
    11101 acgaacgatg gcgaacgaac aaaggcaaac aggtagccac tcaacacctc cggggatggc
    11161 ctgagaacgg agaagcctgt tgaacaaatc tggtcatcga gattgtgtgg aacaagagcg
    11221 accgcgttta ggtttggtcg aacagtcgag acaatgacat ccccagtacg tatgactttt
    11281 ctagcccggc ttggcgcgtc agcgccaata accgtctgcg ctgtgactat ggactttgcc
    11341 ctgttgtcta cgctggcgat atcgacgtat agaaactcat tggacggact gtcccttgga
    11401 tcgcgctgtt cggtaggcag catcaattcg cccaagcgtt ttatcggcca acgtttcatg
    11461 gcttgtgccg aacctcactg agcaagtcag tggcccgctt gcttatctcc ccctctatct
    11521 tcagaatgtc ggccagaatg tccgcgggtg aatcgtggtt cacggcttgc tgtgttaccg
    11581 gtctgtatcg tcccgccgcc aaggtcaggt cattctcttc gattcgttca agcgataccc
    11641 ggtagctgtt cggaccttct tcgtgaccag gccacttcga cagcacatcg ggaatgtcgt
    11701 tggcatcaat gggcgtacgc ttgtcgtcga gactgaaccc gtcggcggtg aggtcgtaga
    11761 accagacttc cttggtcctg gttcctttct ggaatatcag cgccgccgtg gcgactccgg
    11821 catagggccg gaagacaccg gacggcaggc tgacaaccgc ctgaaggtcg caatcgtgga
    11881 ggagcatttc acggactcga cgggcagcct tgttggagcc gaatagaaca ccgttcggga
    11941 caatcacgcc cgcgcgtccg ccgggcgcga ggtggtcaac gaaccatttc acgaagagaa
    12001 gttccgtcgc ccgcgtgtta aggccgtgat tcagattgga cagaatgctt tcctgttgaa
    12061 ccttgccaga gaaaggagga ttcgccagaa caacgtcgta ggtcattccg ggatactgcc
    12121 cggtcttgtt ggtcgtcagc gggttgtgaa attcgatgcc tgccttttcc agcttgtgca
    12181 ggtacagatt caagatggcg attttcacca tgttggcgtc attgtcgtag ccggtaaatg
    12241 cctgctcttc gaggaaacgc cattgagcgg ccttgagcaa atcgccggaa acgtggccct
    12301 tctcaagttc cggcttggag gtgttctgcc tgagaatgtg gttgtaggcc gaaatcaaga
    12361 accccgccgt gccgcaagcc gggtcgcata tccgcatgtc cggtttcggg tcaacgagcc
    12421 gcacgatcat gtcaatgatg tggcgcggcg ttcggaattg gccgtttgtg ccggatgtcg
    12481 agagtttgct cagcaaatac tcgtacatgt cacctttggg atcgtcgccg ttcccggtgt
    12541 cgaacgacat ttcgtgaatg ccttggatca cggcgcggag cgtcgggcgg tcgtagattt
    12601 tcaacgtggc gcgattgaac agaagtttgc cggtggccga catgccggga agctcgtgaa
    12661 tgctttcaat ggccgcgcgg atgctgtcga aaagctggtc gccggtgagg gtgacgaagt
    12721 tgccccaggc ataccgcgcc catttgtcgg tgaatatgcg tttgtacttc ttgtccagct
    12781 tggcgagcag gtcgtctttc tcgcttagca accgcaagaa gatgaggtag gacatctgct
    12841 caatggcgtc catcgggttg ttgagtccgc ccgcccaaag aatatccatc aacgcgtcaa
    12901 ccttttgacg taatcccgat gactgaaagc tgtttccgtt tccgttcatg ccgcctcgat
    12961 tccaagcaat gattgacgca caagggttga ctgcctgagt gcttcaaagg cttcgtcccg
    13021 gtgctcgaac cgcgcaagtt ggccgagtcc acccaagtga ctgaattggc tactgtcgaa
    13081 aacggtcatg tcgccctgaa caaagcgccg aagattctgc ggatcgtccg cgatgagttg
    13141 cgcggttgct gacagccatt tggcttccga gtaccggaga ttgaaccggg ccgcgatgga
    13201 atcaacggtg tccgtgacaa tggcgtcttt cgtgggcaga ggtttcttgc ccaacgcgct
    13261 gtaaacaaaa gcaggcgtcg gggccggaac gccgtaggat ttgatgagct tatccgggga
    13321 atagaacatc tccggcttgt ggaaaaagcg ttcctgcaag atcgtctcga cggtgtcgtc
    13381 gtcttcgacc tccaccgcct cctgaaactc ccggtccttg cccgcaaaga ctttcacgtc
    13441 cttctcaaac gagccgcgga aggtcatcac atcaactttc tcgccgttcg gtccgacaat
    13501 gcggatttcc tccgacacaa tcacgtcacg gccttcccac acggggattt cccgtttctc
    13561 tggctcctcg gctcccggct tggcttcgta cttgccaccc gcttcggcga caaatgcagg
    13621 cttcttctcc ccggtcgctt cggcctgtgc ttgctctccc cgcgcacgcg gcaaggccaa
    13681 cggcaaggag tagtcgtatt cttcctcgaa atactcggcc actccgcaga aatccaagag
    13741 gaagtagtgc ttcttctcgt actcggtatt gccaacgcgg aaagtgaagc gccgcgttcc
    13801 ccggcctttg atctgaatgt attcggtggg cgagaatatc ggtcgcatca aaacgacatt
    13861 gagcaagtcg cggcagttgt agccggttga aagcatgtcc accgagacgg cgacgcgctc
    13921 ggcgcgtttg ccgtcccgga agtctttggc aagatcactc gataccggaa tccgcgatgt
    13981 gatcgtcatg gcaatgcccg gttccagcgc gttcagaatc ttggtcaggt tcgtggcgtg
    14041 atcctgattc accgcaaaga taatggattt tcccagcttc cccgccgggt ctttctgcac
    14101 gtgcttcaag aacgcttcgc acataacgcg gttgcgctgg ggtgtgaata tcttccgctc
    14161 caaatcctga accttgaagt tttcctcttg ctcgctaatc agcacagtcc agccggattc
    14221 ttccagcgcc tttgtggtta tgtcggaacg aatatcgaaa atcttgggca tgcacaggaa
    14281 ggggccttcc gggtccttca cggcttcaat gatgtcgtag cggaaagtcg ggatgccggg
    14341 ctcgcaaccg aaatagtggt aggtatcgcg aaggattctc gcctcaagag ctttcgggtt
    14401 ctcctggctg agctgttcgg cgttgatgtt cttcagatac gctttcggag tagccgtcag
    14461 tccgatgcgc gttgcctgga agaactgcac aacctcgcgg gcgtcgccat aaatcgaacg
    14521 gtgcgcctcg tcgttgatga cgaggtcgaa atgaaatggc gtgaattcct cgcgatagcg
    14581 gcgatccacc atcagggatt gaagcgttgc cacaaccaca caagagccaa gcaattcggc
    14641 gggcttgcgc cgggccgttt tgaaaattac cgggttgaat tcccggagaa tgacgttgaa
    14701 gtcctccatc gtctgcttgg cgagttcgat tctgtccacg atgaaaagga cgcgctctgc
    14761 attccgtgtt gtcaaaaagc ggcgaatcag agcggcgcaa agcagggtct tgccggtgcc
    14821 ggtggccatt tcgagcagga acttgcgttt cctctgctga tcgaactggc gggcgacctc
    14881 gtcaatggat ttgatctgat aaccccggac gatcacgtcg ggcttgaatg ctctcaagta
    14941 gtcagggcca accttctccg agaggagcgg ccggggcggt tgcagattct tcaggagcag
    15001 acgttcaagg tctttcgggg acggtaaccg ttcaatgcgg aaggcgtccg cgtcgccgcg
    15061 ttggaggttc caaaaccagt gttcgcggcc atttgagagg atcacaaacg gggcgcgaac
    15121 attctcggcg taaccccggc cctgttcctt tgcctcgtac gggtcgaggt cttcgcgctt
    15181 ggcctccaag acgcagagcg ggccacgcat tccaagcaga agatagtcgg ctcgcccgtc
    15241 gttcccgcgc agttcaaagc gaaccgcttg gggatcgagc aagtcccatc ctgcctcgcg
    15301 caaggcttga tctatcaaga cgcgcgaaaa tgcctctggt tgtccactca agtcgttcct
    15361 cgtttttcaa taaacacgaa cgggcgcatt atgaacgaac aacggccaca aaaccagccg
    15421 ggattttcaa aactgcgaaa catgttcgca agcgccccac ttcctttccg gcgcttgcgg
    15481 gcatgtttct accgcatccc gtgaacgtag cgcacagcaa ccggcatgac acggcgttgc
    15541 gccgtggcgt gacggatgcg cgcgaagtga acccggcgaa gccggaccgc ggagttctgc
    15601 cgcacggcag aacgacccca gagcgggcca gcttgcgcca gcccgcacgg tgccgggcgc
    15661 gagaattgag aagagcaccc ggcaggtctt gccgccgatc aggcaaatgg ccgtgacgtt
    15721 tgcaccgtag gcgcacactc gcggcgaatt gcccggcggc attggggggc cgtgtttgaa
    15781 gtgacggctt tcgacgggtg gagttttcgg agcacggcgg aaacggcact tgaaacatgg
    15841 ccggggtggg aatcaacgag cgaagcgaga ccgcattcga tgaggtgttc agacattgga
    15901 agaagatgac gccgaaaatc cggacattgg aaaacgcgac agcggctaag gcgcgaaaag
    15961 ctccggcgtt cagccggagc caagcgccgc acgaagcaag ctgtgcggtc gttcagaccg
    16021 cacggcttgc gctcttttct tggctggttg ttgccgccca aggcggcatt ggggtggttg
    16081 gtgagtggag cccgtcagtc cgcagcctct tcctcttcgc gcgcgccccc gaaaacgaag
    16141 acgagaagac ggg
//
LOCUS       DAOOTK010000068        15993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2494_length_15993_cov_4.873447, whole genome shotgun sequence.
ACCESSION   DAOOTK010000068 DAOOTK010000000
VERSION     DAOOTK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15993
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_15993_cov_4.873447"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2364)
                     /locus_tag="PK634_08705"
     CDS             complement(<1..2364)
                     /locus_tag="PK634_08705"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97854.1"
                     /translation="MRKRPGIIAAAALLALGVFSARGDLWPRHAEDRRQALQAGRAAL
                     EDGLYDVAEKHLEQYVRLASGRKDRSEGTLLLARVLYGQGRHEDVVRLLTERASWARG
                     TESEAGFVLWQALALYEMARYEEVLRLVADFKERFPESPYRPQAARLRTKCLVKMQEV
                     SEALQAFAKFQETYPESPEIPANLLDWAGTLIRLGRKDEARQVFDQLLNKYPDSEAAA
                     RGQLWLGGVYTEMGDWEMAREALSTLAAQEQARSEYRTSAWLALADLHESRTNWPAAV
                     EALEQVVALNHDPTTTARARGSRGRLLVRLGRNKEGIAEVKAVIALGLADPAPRDLQL
                     ALARALLDASEWEFAEREFQYYLEAFHDPGQLAEAWMGKGWSLWGLKRYPEAAAAFEQ
                     AADAALNPDLKARALYKVGDCHFAAGAYQQALDRYAQVASNSADAVLSADARFQSAES
                     LELLNRLEEAEALFSQLAQDASDPARAERARLRMARVRERAAKWEAAIEMYGSLLGEG
                     VSAPTRELALHNRGLIRYRLGRYKEAMQDFERVLEEFPAGQTAEQAFYMRGWCLYRLG
                     KDREALTVCRKFLAEYPQSAWAPDVLFWLGEYFFNRGEYAEAEKNFARLAELFGSGDL
                     ADDALYWAGRSAALRKEYVQAIEYFNRLAKDWPDSPKLPDARFAQGDALTELGQFSGA
                     ILAFDEVIRKFPDSALADAAWGRKGDCLFTLGKDDPRRYAEALEAFRMVLKSPAASAD
                     QKWQAEFKMARCQEKAGLPDEAVASYMNVVYGYLTALGKGKAGHPLWF"
     gene            complement(2361..5207)
                     /gene="uvrA"
                     /locus_tag="PK634_08710"
     CDS             complement(2361..5207)
                     /gene="uvrA"
                     /locus_tag="PK634_08710"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOW97855.1"
                     /translation="MSTDHILVAGAREHNLKDITVRIPRNALTVVTGLSGSGKSSLAF
                     DTLYAEGQRKYVESLSAYARQFLEQMQKPEVDHIEGLSPAIAIEQRTAGANPRSIVAT
                     TTEIHDYLRLLYASVGHAHCWKCGRPISRQSAEEIVEQLLKLPPNTRLTLLAPLIRGR
                     KGQHEEVLEAARKQGFVRARVNGEMIELDQAPDLDKRKKHTIEVVVDRLVLNDKIRAR
                     LTDSVELALKQGEGLLQALYPAADGKTVEQLYSEKHACPHCELSFDALTARHFSFNSP
                     YGACPTCLGLGTMLVFDEDLIVPDPNLTLDEGAVRAWKGGGRRTAIYYKGLLRAITRH
                     GQIDMETKYRDLPESFREILLHGTGEEEVEFGFWRGGAYHKYRKPFEGVIPNLRRRYE
                     QTDSDYMRQKLAGFMSRQPCTACRGARLRPEAMACLVGDQSIVDVTRMSIKRALEFFS
                     ALPLTEQEQKIAREVIKEIRERLTFLVDVGLDYLTLDRESGSLSGGEAQRIRLATQVG
                     AGLVGVLYVLDEPSIGLHQRDNRKLIDTLLGLRDLGNTVVVVEHDEQTILSADHVIDL
                     GPGAGRHGGEVVFAGSVPELMKSPQSLTAKYLRKEVEIHVPRVRHAPPGEWLEIQGAA
                     ENNLKNINVRIPLGLLVCVTGVSGSGKSTLVDEILRRALFRRFYGSRERPGRHKKITG
                     MEYLDKVIVIDQSPIGRTPRSNPATYTGAFSLIRDLFAQLPASRVRGYGPGRYSFNVK
                     GGRCERCKGDGILRIEMHFLPDVYVTCEQCRGQRYNRETLEVRYRGKNIAEILALTID
                     EAVEFFQPIPRLARKLVTLSEVGLGYLQLGQPATTLSGGEAQRVKLSAELSRQDTGRT
                     LYLLDEPTTGLHFADVHRLLKVLERLRDAGNTLVVIEHNLDVIKTADYVIDLGPEGGD
                     AGGEIVACGTPEELVASPASHTGKYLAEAMGLKK"
     gene            complement(5380..6192)
                     /locus_tag="PK634_08715"
     CDS             complement(5380..6192)
                     /locus_tag="PK634_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97856.1"
                     /translation="MLAAASIVASLVYLNRAARAEQQAAAARAEADTLRARVSASAAV
                     VAPAPEDSPAEVPAIAVARADDSEAQARIRELETALAEKDRVIWDLQARATNRPPPED
                     RRGRDRGDWMEDLRQRDPARYEEMQKRREERQREVSRQYAEKAAYFLKRDTSKMTDEE
                     KASYDLMLQLLDETWRLSEQMRSELSPEQRRELGQALREKARVLNPLLEEERARTFIE
                     LGEQVGYAGAAATEFADYLESVIDLTSPRGMFRGGPPGGRGPGGSGGAPPGP"
     gene            complement(6328..6972)
                     /locus_tag="PK634_08720"
     CDS             complement(6328..6972)
                     /locus_tag="PK634_08720"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOW97857.1"
                     /translation="MFTGLIQKTGRVLSVNVTAEEGRLVVQASPWETPLAVGESIAVS
                     GACLTLASFQGDRLTFDALQETFQRTNLGRKKPGDRVNLERALRAGDALGGHLVTGHV
                     DGIGIVRGARNAGRDWVLEVEVAEALRDGLVPKGSVTCDGISLTVVSLSDRTFSVHII
                     PHTWTHTTLADLNPGDPVNIETDILGKYVKRLLSSGGGGSSVTMDSLRRAGFIN"
     gene            complement(6976..8058)
                     /gene="ribD"
                     /locus_tag="PK634_08725"
     CDS             complement(6976..8058)
                     /gene="ribD"
                     /locus_tag="PK634_08725"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOW97858.1"
                     /translation="MKHELYMRRALDLGRRGEGLTRPNPAVGAVVVRNGRIVGEGWHR
                     KAGGPHAEVYALRRAGKRARGATLYVTLEPCCTQGRTPPCTEAILKAGIAEVIVGTRD
                     PNPAHAGRGLRLLRRAGVRVVAGVLEGEARALLAPFAKWILARRPFVTLKMGMTLDGR
                     IADETGASRWITGPAARRKVQTLRRRVDGVLVGKNTLLKDNPSLLPVPAKGRKPWRIV
                     LAPDGRVPASAKVLSDGHADQTLLTVSNTCASARVKALQAAGARVLRIRAKAGRLPAG
                     ALLDGLGQLGLLHVLCEGGGELAASLIEGGLVDNYLFFISPSFLGGRATAVVGGRGWR
                     MAGRPRLRFISVERCGPDIMVKALPE"
     gene            complement(8055..8978)
                     /gene="ftsY"
                     /locus_tag="PK634_08730"
     CDS             complement(8055..8978)
                     /gene="ftsY"
                     /locus_tag="PK634_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOW97859.1"
                     /translation="MVTWIDALARTRRQIAGVLSRVFSGRKKEDVSFEELEETLLLAD
                     VPVQLATKLVAEMEQAYAGLRVSRREMLRQILRDSLKGVGEGPFSWPPQPRPLVILVV
                     GVNGSGKTTTSAKLARRALEAGLNPLLGAADTFRAAGVDQLKWWADHLDVESVAGAPN
                     ADAAAVAYDALDAAIARKKDALIVDTAGRMHTKQPLMQELQKVRGALNKRLPGAPHET
                     WIVLDATLGQNALAQARVFHEAVPLTGAIVAKLDGSAKAGFVFGVTRDLGIPIRFAGL
                     GESADSLAPFDPDEFVDALLGKEAETEPAGT"
     gene            complement(8962..9432)
                     /gene="nusB"
                     /locus_tag="PK634_08735"
     CDS             complement(8962..9432)
                     /gene="nusB"
                     /locus_tag="PK634_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOW97860.1"
                     /translation="MGGRHEARQWAVQFLFQRDFNQDDLEPALELFWAGVKGAPSARR
                     FAEELIRGVDTRRAELDQLIQKYAENWDLKRMGAVDRNVMRVGLYEMLHCPDVPPVVS
                     INEAVELAKELSGSESGKFVNGILDRARKDIDRPARAAHPAAGKKEKLARGHVD"
     gene            complement(9440..9910)
                     /gene="ribH"
                     /locus_tag="PK634_08740"
     CDS             complement(9440..9910)
                     /gene="ribH"
                     /locus_tag="PK634_08740"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOW97861.1"
                     /translation="MSTKEYAGQLNAAGLKFGIVVSRFNDFLTRQLLAGAVDCLSRHG
                     AAEADIAVAWVPGAYEIPLAAQKLAQGKKYSAVVALGAVIQGATPHAELINTQLARAL
                     MQIALESGVPVIDGVVAAENLDQAIERCGTKSGNRGWNAAQSAIEMANLLGNWK"
     gene            complement(10018..11241)
                     /locus_tag="PK634_08745"
     CDS             complement(10018..11241)
                     /locus_tag="PK634_08745"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOW97862.1"
                     /translation="MSKEDREKAGPFSPIPEAVEAVRNGELVVVVDDEKRENEGDLVL
                     AAEKVTPEGINFMAKHGRGLICVSMEKEALQRLHLTRMGPPGEGGRFATAFMQSVDAR
                     EGVSTGISAFDRAQTVRVLIGKNTRPEDLVRGGHVFPLEASPGGVLRRTGHTEAAVDL
                     ARLAGLDPSGVICEIINDDGRMARLPDLLAFAARHGLKIITIADLVAWRRKREKLVEL
                     ERKVSLPTEAGRFDLYLYRSLPDEHHHLALVMGRPAEQPAPLVRVHSECLTGDVFGSL
                     RCDCGTQLKAAMEMIAREGHGVVLYMRQEGRGIGLAKKLHAYELQEAGLDTVEANQKL
                     GFDADLRDYGIGAQILADLGLRRIRLLTNNPRKIIGLKGHGLEVVERVALVLPPTEHN
                     ERYLETKKTKLGHWL"
     regulatory      complement(11289..11454)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11601..12404)
                     /gene="lpxA"
                     /locus_tag="PK634_08750"
     CDS             complement(11601..12404)
                     /gene="lpxA"
                     /locus_tag="PK634_08750"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOW97863.1"
                     /translation="MNIHPTAVVDHGAVLGADVVVGPFAVIDRDTQIGDGCVIGPHAV
                     IHRYTSIGPGCRIHAGAVLGDLPQDLAFKPDTVSYVRIGARCWIREGVTIHRGTKPDT
                     VTEMGDDCFLMVNSHLAHNVKLGNGVILVNGALLAGYVEVGDRAFISGNAVVHQFVRI
                     GRLAMMGGQGAVSQDIPPFCTGRSGAGNQILGLNVVGLRRAGFKPESRQALRAAFKIL
                     YRAGLNTSQAVARIRAELNDAAVTEFCDFIAASKRGICGLLESAEDAGE"
     gene            complement(12404..13141)
                     /gene="radC"
                     /locus_tag="PK634_08755"
     CDS             complement(12404..13141)
                     /gene="radC"
                     /locus_tag="PK634_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOW97864.1"
                     /translation="METLYPIAPRRVADIPSRQRPRELFERLGAEHVSEEVLIALLLR
                     CGMKGLNVVDLAHNLLMEYGSLTALSQAPVQELTKKKGIGPVKAQILRAALELGRRLN
                     EESAPERPFVRTPEDAARVLRGRASARETEAFWVLLLDTKNRLRQNPVEISTGLLNAS
                     LAHPREVFKEAIRCLSAAVVLVHNHPSGDPAPSAEDIRITRQLVEAGRIVDIPVLDHI
                     ILGRAAEPGAKDYYSLRESGVVSFEDK"
     gene            complement(13144..14472)
                     /gene="hflX"
                     /locus_tag="PK634_08760"
     CDS             complement(13144..14472)
                     /gene="hflX"
                     /locus_tag="PK634_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOW97865.1"
                     /translation="MGRTPLSFRDEPERVLLVGVRLKDQRRDVVEDLLDELAQLADTA
                     GADVIGRLVCNQQTFNPATLIGKGKAAELAERAAAEDITTILFDDDLSPAQGRNLADI
                     TGCKVVDRAQLILDIFAQHARSKAGRLQIELAQLQYLLPRLRGMWTHLERQQGGIGLR
                     GPGEKQLELDRRRILDRITRIRASLEEVRQHRAEQRRGRRRHGWALLTLVGYTNAGKS
                     TLINHLADAQVEADDRLFATLDPTTRQIRLPNHQPALITDTVGFIRKLPHHLVESFKA
                     TLEEVIEADLLIHVIDAAHPGVEEQIRAVDQVLKELGAEGKPRISVLNKMDLPAAGAQ
                     AARLSPGLERPVTISALTGEGLDMFRAELADFLKSRTLRMQLLVPPAEGKLLAAIRSS
                     GRVFEEKYEADGAVRLDVSLPERLFGLCLPYRLDNGETPPGDGANGLRDP"
     gene            complement(14441..15361)
                     /gene="miaA"
                     /locus_tag="PK634_08765"
     CDS             complement(14441..15361)
                     /gene="miaA"
                     /locus_tag="PK634_08765"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOW97866.1"
                     /translation="MAGSPTAWFLVGPTAAGKSAVAQYLAEREGLDILSADSMLVYRG
                     MDVGTDKPTADERSRVRYWGLDLVEPAEPFSVGRFLEAAREAFRAAAARVIVAGGTGL
                     YVKGLVEGLDAAPPADPDLRKRLSELDVAGLQEELRRRDPARLESLADPRNPRRLIRA
                     IELAECGARGVRPAREPGAPLVGLKPEPARWRARIAERVTRMFEGGLLDEARRLRERG
                     DALSGTARHAIGYAEAFAALDGTLTVGQAREQAVRRTWQLARRQMTWFRHQVRVEWID
                     HDFSEPVARLADRVWAAWRKHGPNPVILPG"
     gene            complement(15361..15861)
                     /gene="lspA"
                     /locus_tag="PK634_08770"
     CDS             complement(15361..15861)
                     /gene="lspA"
                     /locus_tag="PK634_08770"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOW97867.1"
                     /translation="MSVILLAFIIAALDQATKWHVRATFDFGVAHPVIPGFFDLTYVR
                     NTGAAWGMLGGQNAVLTILSVVILVLMVIFRRSFLSRTWEHRLALALLVGGVVGNLLD
                     RVRLGWVTDFLDFHVRGWHWPAFNIADSAICVGAGLYIVSAFWVAGHPLNESRRKTEP
                     AASPES"
BASE COUNT         2365 a         5739 c         5284 g         2605 t
ORIGIN      
        1 aaaccagagc gggtggccgg ccttcccctt gcccagcgcc gtcaggtagc cgtacaccac
       61 gttcatgtag gaggccacgg cctcgtccgg caggccggcc ttctcctggc accgcgccat
      121 cttgaactcc gcctgccact tctggtcggc gcttgccgcg gggctcttga gcaccatccg
      181 gaaggcctcg agcgcctcgg catagcgccg gggatcgtcc ttgcccaggg tgaaaaggca
      241 gtcgcccttg cgcccccagg cggcgtcggc gagcgcgctg tcggggaact tccggatcac
      301 ctcgtcgaag gcgaggatgg cgccgctgaa ctggccgagt tccgtgaggg cgtcgccctg
      361 cgcgaaacgc gcgtcgggca gcttcgggct gtcgggccag tccttcgcca gacggttgaa
      421 gtattcgatc gcctgcacgt actccttgcg gagcgcggcg gaccggcccg cccagtagag
      481 cgcgtcgtcg gccaggtcgc cgctgccgaa gagttccgcg agccgggcga agttcttctc
      541 ggcctcggcg tattcgcccc ggttgaagaa atactcgccc agccagaaca gcacgtccgg
      601 cgcccaggcc gactgggggt actcggccag gaacttgcga cacaccgtca gcgcctcgcg
      661 gtccttgccc agccggtaga ggcaccatcc ccgcatgtaa aaggcctgct cggccgtctg
      721 gccggccggg aactcctcga ggacgcgctc gaaatcctgc atggcctcct tgtagcggcc
      781 caggcggtag cggatgagcc cccggttgtg caacgccagc tcgcgggtcg gcgcggacac
      841 gccctcgccg agcaggctgc cgtacatctc gatcgcggcc tcccacttcg ccgcccgttc
      901 ccggacacgg gccatgcgca gccgggcgcg ctccgcccgc gccgggtccg acgcgtcctg
      961 cgcgagctgg ctgaagagcg cctcggcttc ctccagccgg ttcaggagct ccagcgattc
     1021 cgccgactgg aaccgcgcat ccgccgacag gaccgcgtcg gcgctgttcg aagccacctg
     1081 cgcgtagcga tcgagcgcct gctggtacgc gcccgcggca aaatggcaat cgcccacctt
     1141 gtacagggcg cgggccttca ggtccgggtt caacgccgcg tcggcggcct gctcgaaggc
     1201 ggccgcggct tccgggtaac gcttcaatcc ccagaggctc cagccttttc ccatccaggc
     1261 ctccgccagc tgccccggat cgtggaaggc ttcgagataa tactggaatt cgcgctcggc
     1321 gaactcccac tcgctggcgt ccaggagcgc gcgggccagg gccagctgga ggtcccgcgg
     1381 ggccggatcg gccagcccca gcgcgatgac ggccttgacc tcggcgatcc cttccttgtt
     1441 cctgccgagc cggaccagga gccgtcccct cgatccccgg gcccgggccg tggtcgtggg
     1501 atcatggttc agggccacga cctgctccag ggcctccacg gcggccggcc agttcgttct
     1561 cgactcgtga agatccgcga gcgccagcca cgcggaggtg cggtattccg accgcgcctg
     1621 ctcctgcgcg gcgagggtgg agagggcttc gcgggccatt tcccagtccc ccatctccgt
     1681 gtacaccccg ccgagccaca actggccgcg ggcggcggcc tcgctgtccg ggtacttgtt
     1741 cagaagctgg tcgaagacct ggcgcgcctc gtccttgcgg cccagccgga tgagcgtgcc
     1801 ggcccagtcc aggaggttgg ccgggatttc cggcgactcg gggtacgtct cctggaactt
     1861 ggcgaaggcc tgcagggcct cggagacctc ctgcatcttc accaggcact tggtccgcaa
     1921 ccgcgccgcc tgcgggcggt acgggctttc cgggaagcgc tccttgaaat ccgcgacgag
     1981 ccggagcacc tcctcgtagc gcgccatctc gtacagcgcg agggcctgcc acagcacgaa
     2041 gccggcctcg ctctccgtac cgcgcgccca cgaggcccgc tcggtcagga gccggaccac
     2101 gtcctcgtgg cggccctgcc cgtacaggac gcgggccagc agcagcgtcc cctcggagcg
     2161 atccttgcgg ccgctggcga gccggacgta ttgctcgaga tgcttttcgg cgacatcgta
     2221 caggccgtcc tcgagcgcgg cgcgccccgc ctgcagcgcc tgccgccgat cctcggcgtg
     2281 gcgcggccag aggtcgcccc gggcggaaaa cacccccagc gccagcaggg cggccgccgc
     2341 gatgatgccc gggcgcttcc tcatttcttc aaccccatgg cctcggccag gtacttcccg
     2401 gtatgggagg ccggcgacgc gaccagctcc tccggcgtgc cgcaggccac gatctcgccg
     2461 ccagcatccc cgccttcggg ccccagatca atcacgtaat ccgccgtctt gatcacgtcg
     2521 aggttgtgct cgatcaccac cagcgtgttg ccggcgtcgc gcagccgctc gaggaccttc
     2581 agcagccggt gcacgtccgc gaagtgcagg ccggtggtcg gctcgtcgag caggtacagc
     2641 gtgcgccccg tgtcctgccg gctcaactcg gcggagagct tcacgcgctg cgcctcgccg
     2701 ccggagagcg tcgtcgccgg ctgcccgagt tggaggtagc ccagcccgac ctccgagagc
     2761 gtcaccagct tgcgggcgag ccgcgggatc ggctggaaga actcgaccgc ctcgtcgatg
     2821 gtcagggcta ggatctcggc gatgttcttc ccgcggtagc ggacctccag ggtctcccgg
     2881 ttgtaccgct gcccgcggca ctgctcgcag gtgacgtaga cgtccggcag gaagtgcatc
     2941 tcgatgcgca ggatgccgtc ccccttgcac cgctcgcagc ggccgccctt gacgttgaag
     3001 ctgtagcggc ccggcccgta gccgcggacc cgcgaggcgg gcagctgggc gaagaggtcg
     3061 cggatcagcg agaaggcccc ggtgtacgtc gccgggttgc tccgcggcgt gcgcccgatc
     3121 ggcgactggt cgatcacgat caccttgtcc aggtattcca tcccggtgat cttcttgtga
     3181 cggcccggcc tctcgcggga gccgtagaag cggcggaaca gcgcgcgccg caggatctcg
     3241 tccaccagcg tgctcttgcc gctgccggac acgccggtga cgcacaccag caggcccagc
     3301 ggaatgcgga cgttgatgtt cttgaggttg ttctccgccg cgccctggat ctccagccac
     3361 tcgcccggcg gcgcgtgccg gacgcgcggc acgtggatct ccacctcctt gcgcaggtac
     3421 ttcgccgtca gcgactgggg cgacttcatc agctccggga ccgatcccgc gaatacgacc
     3481 tcgcccccgt gccggccggc cccgggcccg aggtcgatca cgtggtcggc ggaaaggatg
     3541 gtctgctcgt cgtgctcgac cacgaccacg gtgttgccga ggtcgcgcag gccgagcagg
     3601 gtgtcgatca gcttgcggtt atcccgctgg tgcagcccga tgctgggctc gtcgagcacg
     3661 tagagcaccc cgacgagccc ggcgccgacc tgcgtcgcca gccggatgcg ctgggcctcg
     3721 ccgccggaca ggctcccgct ctcccggtcg agggtcaggt agtccaaccc gacgtccacg
     3781 aggaaggtca gccgctcccg gatctccttg atgacctccc gggcgatctt ctgctcctgc
     3841 tcggtcagcg gcagcgccga aaagaactcc agcgctcgct tgatggacat ccgggtgacg
     3901 tcgacgatgg actggtcccc cacgaggcag gccatcgcct ccgggcgcag ccgcgcgccg
     3961 cggcaggcgg tgcagggttg ccggctcatg aacccggcga gcttctggcg catgtagtcg
     4021 ctgtccgtct gctcgtagcg gcgccggagg ttggggatga caccctcgaa cggcttgcgg
     4081 tacttgtggt acgcgccgcc gcgccagaac ccgaactcca cctcctcctc gcccgtcccg
     4141 tgcagcagga tctcccggaa cgattccggc aggtcgcggt acttcgtctc catgtcgatc
     4201 tggccgtgcc gcgtgatggc gcgaagcagg cccttgtagt agatcgccgt ccgccgcccc
     4261 ccgcccttcc acgcgcgcac cgcgccctcg tccagcgtca ggttcgggtc gggcacgatc
     4321 aggtcctcgt cgaacacgag catcgtgccg aggccgaggc aggtcgggca ggcgccgtag
     4381 gggctgttga acgagaaatg ccgcgcggtg agcgcgtcga agctcaactc gcagtgcggg
     4441 caggcgtgct tctcggaata cagctgctcc acggtctttc cgtcggcggc cgggtacagc
     4501 gcctggagca gcccctcgcc ctgcttgagg gcgagctcca cggaatcggt caggcgggcc
     4561 cggatcttgt cgttgagcac cagccggtcc acgacgacct cgatggtgtg tttcttgcgc
     4621 ttgtcgaggt ccggcgcctg gtcgagctcg atcatctccc cgttgacccg cgcccggacg
     4681 aagccctgct tgcgggcggc ctcgaggacc tcctcgtgct gcccctttcg cccgcggatg
     4741 agcggggcca ggagggtcag ccgggtgttc ggcggcagct tgagcagctg ctccacgatc
     4801 tcctccgcgc tctgccggct gatgggccgg ccgcacttcc agcaatgggc gtggccgacg
     4861 ctggcgtaga ggaggcgcag gtagtcgtga atttccgtcg tcgtggcgac gatggaacgc
     4921 gggttggcgc cggcggtccg ctgctcgatg gcgatggcgg gcgacaggcc ctcgatgtgg
     4981 tccacctcgg gcttctgcat ctgctcgagg aattgccggg cgtacgccga gaggctttcc
     5041 acgtatttcc gctgcccctc ggcgtagagc gtgtcgaacg ccagcgagga tttcccggat
     5101 ccgctcaagc cggtgaccac ggtcagggcg ttgcgcggga tacggacggt gatgtccttc
     5161 aggttatgct cccgcgcccc cgccaccagg atatggtccg tgctcatgag ttccgctttt
     5221 tcgggaaaga cttaatcagg aatactaaca caggttgccc ggattgaaaa cggggcaaat
     5281 ggcggcgcgg ccggcttcac cacctcgccg cgaggtaaca aatacattca cgagtgtgag
     5341 gtgatttgtt acggatggag cgcgccccgg agcgggatgt cagggacccg gcggcgcccc
     5401 accggatccg ccgggccccc gcccgccggg cggtccgccg cggaacatcc cgcggggaga
     5461 ggtgagatcg ataacgcttt ccaggtagtc cgcgaactcg gtcgcggccg cgccggcgta
     5521 accgacctgc tcgcccagtt cgatgaaggt ccgggcgcgc tcctcttcca gcaacgggtt
     5581 gagcacccgg gccttctcgc gcagggcctg ccccaattcc ctccgctgct ccggcgacag
     5641 ctccgagcgc atctgctcgc tcaaccgcca ggtctcgtcc agcagttgca gcatgagatc
     5701 gtacgacgcc ttctcctcgt ccgtcatctt cgacgtgtcg cgcttgagga aatacgcggc
     5761 cttctcggcg tactgccggg acacctcgcg ttgccgctcc tcccggcgtt tctgcatttc
     5821 ctcgtagcgg gccggatcgc gctgccggag gtcctccatc caatctccgc gatcgcggcc
     5881 ccggcggtcc tccggcggcg gccggttcgt ggcccgggcc tgcagatccc agatgacccg
     5941 gtccttttcg gcgagggcgg tctccagttc gcggatccgc gcctgcgcct ccgagtcgtc
     6001 tgcgcgggcc accgcgatgg cggggacttc ggccggcgag tcctcgggcg ccggagcgac
     6061 gacggccgcc gaggccgaca cccgcgcccg cagcgtgtcc gcctcggccc gcgcggcggc
     6121 ggcctgctgc tccgcgcgcg cggcccggtt tagatagacg agcgaggcca ctatcgaagc
     6181 ggccgccagc atcacacaga cgcccggaaa cagcctgccg cctcttgaag ccatcctgtt
     6241 cattctgcca tccatcttac tacgcggcga accgcgctat tactgttccc tgctgtcatg
     6301 acgttccaaa tcacgccgcc gctcccttca gttaataaaa cccgcgcggc gcagggaatc
     6361 catcgtcacg ctcgaaccgc cgccgcccga ggacaacagg cgttttacgt acttgcccag
     6421 gatgtcggtt tcaatattga ccggatcgcc ggggttcaag tccgccagcg tcgtatgggt
     6481 ccaggtgtgc gggatgatat ggacgctgaa ggtgcgatcc gaaagggaca cgacggtcag
     6541 gctgatgccg tcgcaggtca cggagccctt cggcaccagg ccgtcgcgga gggcctccgc
     6601 cacctcgacc tcgaggaccc agtcccggcc ggcattccgc gcgccccgca cgatgccaat
     6661 gccgtccacg tggccggtga caagatgccc gccgagggcg tcgcccgccc gcagggcccg
     6721 ctcgaggttc acccggtcgc ccggtttctt ccgccccagg ttggtcctct ggaatgtctc
     6781 ctgcagggcg tcgaatgtca ggcggtcgcc ctggaaggac gccagcgtca ggcacgcgcc
     6841 ggacaccgcg atactctcgc cgacggccag cggcgtctcc cacggggacg cctgcacgac
     6901 caggcggcct tcttcggccg tgacgttcac ggaaagcacg cggccggttt tctggatcaa
     6961 tcccgtaaac atgagttact ccggcaaggc tttcaccatg atgtccgggc cgcaccgctc
     7021 gacggaaata aaacgaagcc ggggccggcc cgccatgcgc cagccgcgcc ccccgaccac
     7081 ggcggtcgcg cggccgccca ggaaggacgg cgaaatgaag aacagatagt tgtcgaccaa
     7141 gccgccctcg atcagcgagg ccgccagttc cccgccgccc tcgcagagca cgtgcagcaa
     7201 gcccagctgg cccagtccgt ccagcagcgc gcccgccggc agccgccctg ccttcgcgcg
     7261 gatgcgcagc acgcgcgcgc cggccgcctg gagggccttc acccgcgccg acgcacaagt
     7321 gttcgatacg gttaaaaggg tctgatccgc atggccatcg gacagcacct tggctgacgc
     7381 cgggacccgt ccgtcgggcg ccaggacgat gcgccaaggt ttccgtccct tcgcgggcac
     7441 cggcaggaga gaaggattat ccttgagcaa tgtgtttttc cccacgagga cgccgtccac
     7501 gcggcggcgc agggtctgca ctttccggcg ggccgccgga cccgtgatcc agcgcgaggc
     7561 gcccgtctca tcggcgatcc ggccgtccag ggtcatgccc atcttcagcg tcacgaacgg
     7621 gcgccgggcc aggatccact tggcgaacgg cgcaagcaac gcccgcgcct cgccttcgag
     7681 aacgcccgcc acgactctca cgccggcccg ccgcagcaac cggagcccgc gccccgcgtg
     7741 ggcgggattc gggtcgcgcg tgccaacgat cacctccgcg atccccgcct tcaggatggc
     7801 ttccgtgcag ggcggcgtgc gtccctgggt gcagcagggc tcgagcgtga cgtatagcgt
     7861 cgcgccgcgc gcccgcttcc ccgctcggcg cagggcatac acctccgcat gcggtccgcc
     7921 ggctttccgg tgccagccct cgccgacaat ccgtccgttc cgcacgacga ccgcgcctac
     7981 cgccgggttc gggcgggtca gcccctcgcc ccgacggccc aggtccagcg cccgccgcat
     8041 gtacagctcg tgcttcatgt cccggccggc tcggtctccg cctccttgcc cagcagggca
     8101 tcgacaaatt catcgggatc gaacggcgcc agcgaatccg cgctctcgcc caggccggcg
     8161 aaccggatcg ggatgcccag gtcccgggtc acgccgaaaa cgaagcccgc cttggcggag
     8221 ccgtccagct tggccacgat cgcgccggtc agcgggacgg cctcatgaaa gacccgcgcc
     8281 tgggccagcg cgttctggcc cagcgtcgcg tcgagcacga tccaggtctc gtgcggcgcg
     8341 cccggcagcc gcttgttcag cgcgccgcgg accttctgca actcctgcat gagcggctgc
     8401 ttcgtgtgca tgcggccggc cgtgtcgacg atgagcgcgt ctttcttccg cgcaatcgcc
     8461 gcgtcgagcg cgtcgtaggc gaccgcggcc gcgtccgcgt tcggcgcgcc ggccaccgat
     8521 tccacgtcca ggtgatcggc ccaccacttc aactggtcga cgccggcggc gcggaacgtg
     8581 tccgcggcgc ccaggagggg gttcaacccg gcctcgaggg cgcggcgggc cagcttggcg
     8641 gacgtggtcg tcttgccgga cccgttgacc cccacgacga gaataaccag cggccggggc
     8701 tggggcggcc atgagaacgg cccctcgccg acgcctttca ggctgtcgcg gaggatctgc
     8761 cgcagcattt cccggcggga aacccgcagg cccgcgtagg cctgctccat ctcggccacc
     8821 agcttcgtgg ccagttgcac ggggacgtcg gcgagcagca gggtctcctc cagttcttcg
     8881 aaggaaacgt cctccttctt gcggcctgaa aacacgcgcg acaggacgcc ggcgatctgc
     8941 cggcgcgtgc gcgcgagggc gtcaatccac gtgaccacgg gccagcttct ccttcttccc
     9001 ggcggccggg tgggccgcgc gggccgggcg gtcgatgtcc ttgcgcgcgc ggtcgaggat
     9061 gccgttgacg aatttcccgg attcggagcc gctcaactcc ttggcgagtt cgacggcttc
     9121 gttgatggac accacgggcg gcacatcggg gcagtgcagc atctcgtaca ggcctacgcg
     9181 catcacgttg cggtccacgg cgcccatccg cttgaggtcc cagttctcgg cgtatttctg
     9241 gatcagctgg tccagttccg cgcgccgggt atccacgccc cggatgagct cttccgcgaa
     9301 ccggcgtgcc gacggcgcgc ccttgacccc ggcccagaag agctccaggg cgggttccag
     9361 gtcatcctga ttgaaatccc gctggaacag gaactgaacc gcccactgcc tcgcctcgtg
     9421 ccgtccaccc atgggtcacc tatttccagt tgccgagcag gttagccatt tcgatcgcgg
     9481 actgcgcggc gttccagccc cggttgccgg acttcgtgcc gcagcgctcg atcgcctggt
     9541 cgaggttttc cgcggcgacg acgccgtcga tcaccggcac gccgctctcc agcgcgatct
     9601 gcatcagcgc ccgcgccagc tgggtgttga tcagctcggc gtgcggcgtg gcgccctgga
     9661 tcacggcgcc cagcgccacg acggcggagt acttcttgcc ctgcgccagc ttctgggccg
     9721 ccagcgggat ctcgtacgcg cccgggaccc acgcgacggc gatgtccgcc tcggccgcgc
     9781 cgtggcgcga caggcagtcc accgcgcccg cgagaagctg cctcgtgagg aagtcgttga
     9841 aacggctcac cacgatgccg aacttcagcc cggcggcatt caactgaccg gcgtattcct
     9901 tcgtgctcat gtgttgctcc tttcccagca ggtagggcgc gctggcccag cgcgcctcgg
     9961 cggcttgagc caagccgccc tacccagctc tcatttgttg tcttatggcc aacgatctca
    10021 cagccagtgc cccagcttgg tcttcttcgt ctccaggtag cgctcgttgt gttccgtcgg
    10081 gggcaggacc agcgcgaccc gctcgacgac ttccagcccg tgccccttca acccgatgat
    10141 cttgcgcggg ttgttggtca gcagacggat ccggcgcagg ccgaggtccg ccaggatctg
    10201 cgcgccgatg ccgtagtccc gcaggtcggc gtcgaagccc aacttctggt tcgcctccac
    10261 ggtgtccagg cccgcctcct gcagctcgta ggcatgcagc ttcttcgcca ggccgatgcc
    10321 ccggccctcc tgccgcatgt agagcaccac gccgtgcccc tcgcgcgcga tcatctccat
    10381 cgccgccttc agttgcgtgc cgcagtcgca gcgcagcgag ccgaagacgt cgcccgtcag
    10441 gcactcgctg tggacgcgca ccagcggcgc gggctgctcg gcgggccggc ccatgaccag
    10501 ggccaggtga tggtgttcgt ccggcagcga gcggtagagg tacaggtcga agcggccggc
    10561 ctccgtcggc aggctgacct tgcgctcgag ttcgaccagc ttctcgcgct tgcggcgcca
    10621 cgccacgagg tccgcgatgg tgatgatctt gagcccgtgc ctcgcggcga acgccagcag
    10681 gtccggcagc cgcgccatgc ggccgtcgtc gttgatgatc tcgcagatca cgcccgacgg
    10741 gtccagcccg gcgagccggg ccaggtccac ggccgcctcg gtatggccag tccgccgcaa
    10801 cacccccccc ggggaggcct ccagcggaaa cacgtgcccg cctcgcacca gatcctcggg
    10861 ccgggtgttt tttccaataa ggacgcggac ggtttgcgcc cggtcaaaag cgctgatgcc
    10921 ggtcgatact ccctcgcgag catccaccga ctgcatgaag gcggtggcga accgcccgcc
    10981 ctcgcccggc gggcccatgc gggtgaggtg gaggcgctgc aaggcttcct tctccatgga
    11041 cacgcagatc aggccgcgcc cgtgcttggc catgaaattg atgccctcgg gcgtcacctt
    11101 ctccgccgcg agcacgaggt cgccctcgtt ttcccgcttc tcgtcgtcca cgacgacgac
    11161 cagttccccg ttccggacgg cctcgacggc ttccgggatg gggctgaacg ggccggcctt
    11221 ttccctgtct tctttgctca taaaaaaacg ccccgggaaa tatcccaggg ccaaagcacg
    11281 cgccgcgcct cgatcttctt ccatccagac tttactgtcg gctgcggatc ggccgttttt
    11341 ttcccggcct gaccacatca gtcccgatcg ctcgggagtc gcgggctttc accgccggtc
    11401 aggaattccc cgtcgcgctg ccgcgatcag agtcaccttg ccctgaagat ctttccggag
    11461 acgaacctac tcccgacgcc cccggcgcgc aagcgtgaag cgagccatcc cggtgaaaca
    11521 tattcagtca cggccgtgaa gtaatatgtt acagaaggaa acggccacgc gagggccggg
    11581 ttcaggccgg cgcgatggaa tcactcgccc gcatcttccg ctgattccag cagcccgcag
    11641 atgccgcgct tggaggccgc gatgaagtcg cagaattccg tcaccgccgc gtcgttcaat
    11701 tccgcccgga tgcgcgcgac cgcctgcgag gtgttgagcc cggcccggta gaggatcttg
    11761 aacgcggcgc gcaaggcctg gcgtgactcg ggcttgaagc ccgcgcggcg cagcccgacg
    11821 acattgaggc ccaggatctg gttccccgcg cccgagcgcc cggtgcaaaa cggcgggatg
    11881 tcctgggaga ccgccccctg cccgcccatc atcgccagcc tcccgatgcg cacgaactgg
    11941 tggacgacgg cgttgccgct gatgaacgcc cggtcaccca cctcgacgta cccggccagc
    12001 agcgcgccgt tgaccaggat cacgccgttc cccagcttga cgttgtgcgc gaggtgcgag
    12061 ttgaccatca ggaagcaatc gtcccccatc tccgtgaccg tatccggctt cgtgccgcgg
    12121 tggatggtca cgccttcccg gatccagcag cgcgcgccga tgcggacgta gctgaccgtg
    12181 tcgggcttga aggccaggtc ctgcggcagg tcgccgagca ccgcccccgc gtgaatccgg
    12241 cagcccggcc cgatgctcgt gtaacggtgg atgaccgcgt gcgggccgat gacgcacccg
    12301 tccccgatct gcgtgtcgcg gtcgatgacc gcgaacggcc ccacgacgac atccgcgccg
    12361 agcacggcgc cgtgatccac gacggcggtt ggatgaatgt tcattatttg tcctcgaagc
    12421 tcacgacccc gctctcgcgc aggctgtagt aatccttcgc ccccggctcc gcggcgcggc
    12481 ccaggatgat atggtccagc accgggatgt ccacgatccg tcccgcctcg accagctgcc
    12541 gcgtgatccg gatgtcctcg gccgacggcg cgggatcgcc ggacggatgg ttatgcacga
    12601 gcaccacggc ggcggacagg caacggatcg cctccttgaa cacctcgcgc gggtgggcca
    12661 ggctggcgtt gagcaggccc gtgctgatct cgacgggatt ctggcgcagg cggttcttcg
    12721 tatccagcag caggacccag aacgcctccg tctcgcgtgc gctggcccgg ccgcgcagga
    12781 cccgcgcggc gtcctccggc gtgcggacga acgggcgctc gggcgcggat tcttcgttca
    12841 ggcggcggcc aagttcgagg gccgcgcgca ggatctgggc cttgacggga ccgatgccct
    12901 tcttcttggt cagttcctgg accggggcct gggagagggc ggtcagcgag ccgtattcca
    12961 tcaggaggtt atgcgccagg tcgacgacgt tcagcccctt catgccgcag cgcagcagca
    13021 gggcgatgag cacttcctcg gacacgtgct cggcgccgag ccgttcgaat agctcccgcg
    13081 gccgctgccg cgacgggatg tcggccacgc gcctcggcgc aatgggatag agcgtttcca
    13141 tggtcacggg tcgcggaggc cgttcgcgcc gtccccgggc ggcgtttccc cgttgtccag
    13201 ccggtacggc aggcacaggc cgaacagccg ctccggaagg ctgacgtcga gccggaccgc
    13261 gccgtcggcc tcgtatttct cctcgaacac cctaccggaa ctccggatgg cggcaagcaa
    13321 ttttccctcc gcgggcggaa cgagcagctg catgcgcagc gtccggctct tgagaaagtc
    13381 ggcgagctcg gcccggaaca tatcgagccc ctcgccggtc agcgcggaga tcgtcaccgg
    13441 ccgttcgagg ccgggcgaca gccgcgcggc ctgcgcgccc gcggccggca ggtccatctt
    13501 gttcagcacg gatatcctgg gcttcccttc cgcgccgagt tctttcaaca cctggtccac
    13561 ggcacggatc tgctcctcga cgcccgggtg ggcggcgtcg atgacgtgga tcagcaggtc
    13621 cgcctcaatg acctcctcca gcgtcgcctt gaacgactcg acgagatggt gcggcagctt
    13681 gcggatgaac ccgaccgtgt cggtgatcag ggcgggctgg tggttcggca ggcggatctg
    13741 ccgcgtcgtg ggatcgagcg tggcgaagag ccggtcgtcg gcctcgacct gcgcgtcggc
    13801 caggtggttg atcagcgtgg atttgcccgc gttggtatag ccgacgagcg tcagcagggc
    13861 ccagccgtga cggcggcgcc cgcgccgctg ttcggcgcgg tgctggcgca cctcctcgag
    13921 ggaggcgcgg atgcgcgtga tccggtcgag gatccggcgc cggtccagct ccagctgctt
    13981 ctcgcccggg ccgcgcaggc cgatgccgcc ctgctggcgc tcgaggtgcg tccacatgcc
    14041 gcgcaggcgg ggcagtaaat actggagctg cgccagttcg atctgcaggc ggccggcctt
    14101 cgagcgggcg tgctgcgcaa agatatcgag gatcagctgc gcccggtcga cgaccttgca
    14161 gccggtgata tccgccaggt tccggccctg cgcgggcgag aggtcgtcgt cgaagagaat
    14221 cgtcgtgatg tcctcggccg cggcgcgctc cgccagttcg gcggccttgc ccttgccgat
    14281 cagcgtcgcg ggattgaacg tctgctggtt gcagaccagc cggccgatca cgtccgcgcc
    14341 ggccgtgtcg gccaactgcg ccagctcatc cagcaggtcc tcgacgacat cccggcgctg
    14401 gtccttgagc cggacgccga ccagcaggac ccgctccggc tcatcccgga aggataacgg
    14461 ggttcggccc atgcttcctc catgccgccc atacccggtc cgcgagccgg gcgacgggct
    14521 cgctgaaatc gtgatcgatc cactccacgc ggacctggtg ccggaaccag gtcatctgcc
    14581 ggcgcgccaa ctgccaggtg cggcgtacgg cctgctcccg cgcctgcccc acggtcaggg
    14641 tcccgtccag cgccgcgaag gcctcggcgt agccgatggc gtggcgggcg gtgccggaca
    14701 gcgcatcgcc gcgttcgcgc agtcgccggg cctcgtcgag cagcccgccc tcgaacatcc
    14761 gcgtcacgcg ctcggcgatg cgcgcccgcc agcgggccgg ctccggtttg aggccgacca
    14821 gcggcgcgcc gggttcgcgc gccggccgaa cgccgcgcgc gccgcattcg gccagttcga
    14881 tggcgcggat cagccggcgc ggattccggg gatcggccag cgattccagg cgcgccgggt
    14941 cgcgccgccg cagctcctcc tgcaggccgg cgacatccag ttccgaaagc cttttgcgaa
    15001 ggtcggggtc ggccggcggc gcggcgtcga gcccctcgac gaggcccttg acgtacaggc
    15061 cggtgccccc ggccacgatc acgcgggcgg cggctgccct aaaggcctcc cgggccgctt
    15121 cgagaaaccg gccgacgctg aacggctcgg cgggctcgac gagatcgagt ccccagtaac
    15181 ggacccggct gcgctcgtcc gccgtcggct tgtcggtgcc gacgtccatg ccccggtaaa
    15241 cgagcatcga atcggcggag agaatatcga ggccttcgcg ctccgcgagg tattgcgcga
    15301 cggctgactt tccggccgcg gtcggcccga cgagaaacca ggcagtcggc gatccggcca
    15361 tcaggactcc ggagaggcgg cgggctccgt cttgcggcgg gattcattga ggggatggcc
    15421 ggccacccag aaggcggaca cgatgtacag gcccgccccg acgcagatcg cggaatccgc
    15481 gatgttgaag gccggccagt gccagccgcg cacgtggaaa tccaggaaat cggtgaccca
    15541 gccgagacgc acgcgatcaa gcaggttgcc cacgacgccg ccgaccagca gcgccagagc
    15601 caggcgatgc tcccacgtcc ggctcaagaa cgaccgccgg aaaatcacca tcaggaccag
    15661 gatgacgacc gaaaggatgg tcaggacggc attctgtccg ccgagcatgc cccaggctgc
    15721 gccggtgttg cggacgtagg tcaggtcgaa aaagcccggg atgaccggat gcgcgacgcc
    15781 gaagtcgaag gtcgcgcgca cgtgccactt tgtcgcctga tccagcgccg cgatgatgaa
    15841 agccagcagg atcaccgaca taggcccgct cctggtttcc cgggtcgggt caggattccc
    15901 cgccctgctg gatggtcggg ccgaacggac gaaacttgct gcggcccttt tccgattcgc
    15961 tcttgcagtg cacgcacatc ctcgcgaacg gca
//
LOCUS       DAOOTK010000069        15946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2503_length_15946_cov_5.455918, whole genome shotgun sequence.
ACCESSION   DAOOTK010000069 DAOOTK010000000
VERSION     DAOOTK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_15946_cov_5.455918"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PK634_08775"
     CDS             complement(<1..1106)
                     /locus_tag="PK634_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97868.1"
                     /translation="MKRMVALISGLLVAGSVQAATLYVWTNSPANGPGTAWSNAFHTI
                     QAAVDAAGAGGLVLVTNGMYSVGGRATPGGTLVSRVCLTNGITVRSMNGPNVTTIAGM
                     FDSTTLSNGPLAVRCAYVSTGTVLSGFTLTFGHTGNSGNWPFDTGGGGALLMGGTISN
                     CVLSGNQAHADGGGAGVWYGGEIWNSRIESNFVVTANGGGAYVRYGSLLRNCLFRGND
                     ASASSGTGLGGGLCISSGGGSVENCTIVSNTAKQGGGVFTYQNGTNLNDIIWGNTATT
                     SGDNWQFNTTGRYEYCCTTPLTGLPYGTGCISNDPDFVDGSYHVRFNSPCVDAGTNLP
                     WTATAKDLDGSNRLVGACVDLGCYEAPFVLRVTHT"
     gene            complement(1119..1826)
                     /locus_tag="PK634_08780"
     CDS             complement(1119..1826)
                     /locus_tag="PK634_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOW97869.1"
                     /translation="MTPLAAFTPTRSCLLRAAHTLLYARMTKGRGRQARQPEVTRRRI
                     VEAAGRLFAKRGFNGVGFRDIATAARVSLRMPNHHFGTKEQLFAECVRYALTEKMDFP
                     ALFASEPSGTATRIAAKIRACFFAVIPPDGRSSWCGEILGRALTENLNDALKAFQDGL
                     RPAREWFHPALRTIRPRMTPTAFALWYGSLWAQVSFFVTARAAILARLGKRRYDKALV
                     RMAANHLVHGMLKQLEN"
     gene            complement(2940..3016)
                     /locus_tag="PK634_08785"
     tRNA            complement(2940..3016)
                     /locus_tag="PK634_08785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2980..2982),aa:Arg,seq:acg)
     gene            complement(3099..4526)
                     /locus_tag="PK634_08790"
     CDS             complement(3099..4526)
                     /locus_tag="PK634_08790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOW97870.1"
                     /translation="MLFNSFTYAVFLVAVLCVYLRLPLRGRQIFLLVMSYLFYCWETP
                     IYGTLLAISTLLDYYCGLGLARTQNPGLRRLILGCSVAGNLGMLGFFKYGDFFIANTV
                     GLGRLLGFEIDWTPMRIILPVGISFYTFQTMSYTIQMYRRQTEPCRDLINFGLFVTFF
                     PQLVAGPIERANHLLPQLLVYQRVTWEDIGAGLTRIIFGFFRKLVLADRFAILANAVF
                     AAPEGYSTFAVWTSLMAFSLQIYFDFAGYSDIAIGSARLFGVRIVENFRRPLYASSIA
                     DFWNRWHISLTSWLRDYLFYPLGGFRKGGGRALLNGMIVLLLCGLWHGASWHFVFWGG
                     FHAVLLALYYLWRGFRKRMGWKNPSRRLGVSVLFAVAFTYLCTMFSAVFFRAPNVPVL
                     GRMLKIMFGFRAEAGPPTPWVVWGYWAIFVAFALVEFAQEYLDLNDRIRRWPWVARTI
                     ALSMLVAAIVLLAVNNSAPYIYFQF"
     gene            complement(4533..4820)
                     /locus_tag="PK634_08795"
     CDS             complement(4533..4820)
                     /locus_tag="PK634_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97871.1"
                     /translation="MLAEYLDGLKKTGVPAVIVAQPRRALAHDARAQWDEAFIRDLAP
                     QAEARGLPLWDLRDALPDDEFLTSSHLKPPNHERLAGLLCDRIAALETGSP"
     gene            complement(4793..5587)
                     /locus_tag="PK634_08800"
     CDS             complement(4793..5587)
                     /locus_tag="PK634_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97872.1"
                     /translation="MKRRWPWVILIAGIVLGLCEVLARRLLPDVPGSLEENRNPFRFR
                     GWPEFIRDVGHGPKGATVVLISNSQAYAGEYLGRQTYPARLEALLNERRVGGRSDWQV
                     LNWSSDGMTSIEYVLLARYLQLHPPSRILAVTGFADYSLEHANRGFLHCRTDLPRLAS
                     RPAILRGLPRSFLRRHGRIEDGLAYAMYDRVALYRLKEYLWSWLDIRLPGIHSTFYAP
                     AVNYRPWQLKGRAWLPEMPLRARKAGTSSSRTTGRPATCWPSTWTG"
     gene            5700..7262
                     /locus_tag="PK634_08805"
     CDS             5700..7262
                     /locus_tag="PK634_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97873.1"
                     /translation="MNIPPASSETRRARLVGAAFLLLFAAVFLQNAWVCDDAYITFRS
                     IDNFLHGHGLRWNVAERVQSFTHPLWMLLMTAAASVTREFFFTAIGVSLVLCLAALAL
                     LRGGPVLAALLVSSKAFMDYSSSGLENALSYLLLALFLREFLQDAPGEGPRDRRRIGR
                     LVLYATLAYLNRHDAILLYLPALLVIVAAGVRRHGATALVPVLAGGLPAVLWHAFSVL
                     YYGFFFPNTYYAKLSGAGVDAGVLHRQGWAYLANSLRFDPVTLAVTALGLPAFLLSDR
                     RKAAGACVAAGLLLYFAYVICIGGDFMSGRFLALPFLAGALLAARTASARRLAGPALA
                     LLALYHVVAPRAPIKTTRHYARWDADEQHGISDERAYYQDGASLMFYSPARPPPDNAY
                     TRLARQAKGAPDKVFISETIGFAGYLMGPEKFIIDKLGLTDPLLARLPISRRSLANFQ
                     IGHFARQLPAGYFESCADGVNRIEDPGVRAYYDGLQLITRAPLFAPGRWKAIAEYNLG
                     AKRRYDRPYLAD"
     gene            7350..8093
                     /locus_tag="PK634_08810"
     CDS             7350..8093
                     /locus_tag="PK634_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW97874.1"
                     /translation="MRLPLCLMVCLCSLSAAEAAVTNYVETFNDTGDQEHHWMYWGQP
                     TGGGEVADQSVVWMANGGAGGTPRVTVDMENLDYNANGYWLHYLNSPDVGGTRTTDQE
                     LNFNDHIVTAAFNSHNIADPFNGGSVFFFLGQYIDGGNSAWYYFNQAFTVNVTQDDWS
                     QATSIGITGAESEWTLLSGNASISVTNLFNNPQQYGFVIVGAGSQPTYNLSMDNFSAA
                     QVIPEPGVLALLGAGLALLHLKRARSPGR"
     gene            8141..9556
                     /locus_tag="PK634_08815"
     CDS             8141..9556
                     /locus_tag="PK634_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOW97875.1"
                     /translation="MGVREKFFSADTYRNVEGILGIVKDGLCHRCGACIGVCPVGTFG
                     IDEHAYPKQVDKCIECNICVRVCPGLAVDYPALGEALFPGRYEFGSLMGPVLSTWVAH
                     AGEPDIRRRGASGGVITQLLVHWLETGRIKGALVTVEDPEEPSRGKGIIARTREDILR
                     SAQSRYCTAPSFSALYDIRDEEGPFAVVGLPCQIHALRKRQRMDPRWKKRIPMAIGLL
                     CHYNLPFESTRASGRMLAPKGERLAHANFRQRDERGWPHNTLELTFTDGSKWRSPYGP
                     AQIFNIVSRVSPLGRCLMCLDAAAEFSDFSIGDPWIRDEKGLWKYDEPGGWSSVLVRT
                     PEAEQWLREAEQAGKLTMKPVPPAEVERGQHAMMIEKKQRTAFRLRLRKRFGRPVPDY
                     PMPLPGTSAEQKRKEMAFWLTRILPMFPWLARLALRIGLSPFGGYFVRRRMRARQRAA
                     ASGKYLVSASDFGDARERKSG"
     gene            complement(9548..11257)
                     /locus_tag="PK634_08820"
     CDS             complement(9548..11257)
                     /locus_tag="PK634_08820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW97876.1"
                     /translation="MNETPLSRAEGVAREPRDFHWLERNIPCQAACPAQTDIPGYLAA
                     VSQGDFEGAYRINLADNVFPGVLGRVCARPCEPPCRHGWPGLGEPVAICFSKRAAAGH
                     RAPREAVVLSPVFPATGKKVAVVGAGVAGLTVARQLVRLGHEVVVYERHERPGGMMRQ
                     GIPAFRLPRDVVDSEIRQVEAAGVRIACGVNVGGDVSVEQLLADHDAVVLAAGTLRPN
                     IPKGPGVSRKGVEHGLEFLLSVNELGRRDIGRRVAVIGGGFTAIDCARVALRLGAEKV
                     GVFYRRSADDMRVTPGELEEMAEEGIPLETQVAPVAFDGDAGGVRSVRFIRTRPGEPD
                     ADGRRRPVEIPGSEFAVEADLVLLATGQQADRSWAPALASPKLFLAGDYATGATTLIE
                     AIGHARQITREVDAALMGGSRVEDVVRIESGREVARTRAMDAVPRQPMPGLPVDARKL
                     EAEVETGLPADRAREEAGRCYLCHYKYEIDMDRCIYCDQCVEVKPRPDCIVKARALRK
                     DGEGRITGWDRRDEHLLPGTPPFLYWIHQEDCIRCNACLEVCPVKCISVQKVSRREAA
                     ASA"
     gene            complement(11257..12003)
                     /locus_tag="PK634_08825"
     CDS             complement(11257..12003)
                     /locus_tag="PK634_08825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOW97877.1"
                     /translation="MNESNNKLKLEAQAAIHDPGRSALRRYALLTTGGTSLWTLFVHE
                     LVTGLLSGCPGALGIWLRRKLYRRLFAAAGRELIIGRNVTIRGFARIRVGDRVVLDDN
                     SVVDARGAGASITLGDEALVGRNSIVRCRGESLTIGDGTDIGCNCIVATDSRLAIGKD
                     VLVAAYTYIAAGGNHNYADKTVPIRKQGFTSRGGVTIGDDVWIGSHTAILDGVTIGRG
                     AVIGAHSLVNKDLPEWAIAYGCPARVERLR"
     gene            complement(11951..13036)
                     /locus_tag="PK634_08830"
     CDS             complement(11951..13036)
                     /locus_tag="PK634_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOW97878.1"
                     /translation="MSSRGQPLKRRTLLVKAAVSLVFFAVLFAFAGRQDLLAMLKRVD
                     PLYFTLSFVIAAAQISTSCLKWRVLINLYGDRLGFGFLMRNYLIGYYFSNLLPSNIGG
                     DVVRSYYAGRRIGNQTHAAISVFMERFTGSLYLLVLAIGMPLLKPGLYARPVVFVPAA
                     GAAGLLLFFAALAFWGQGLLRFLNRLIERQALRAAGAPGRSLLGRGMKALLWFRAKSE
                     KIAEKMALTMRLLKQNRRAALAVFGLTVLFYALTWVNVYLAYRTFGVEPDFIGIMSLL
                     ATAMLAGSIPITLGSLGIAEGAYVFYFRLVGMEPAATLAMGLFLRFKLLAVGLAGLVS
                     YLTYPHERFDYERIQQQTETGSPGGDP"
     gene            13137..15518
                     /locus_tag="PK634_08835"
     CDS             13137..15518
                     /locus_tag="PK634_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW97879.1"
                     /translation="MDSALISDGAPARRPARLHTRVGLLCLVVLASPAASARAAPGLP
                     EGAGSFPWVPVLLLLLAVVGGTIAWVARRELRRQARDLHRARREMEARGRILAGVAET
                     GALLLGESQVDRGIRRALEILGRAAGVDRVFVTEELAPAGPGRSIAGIRWEWRASPAL
                     AGAGPSAEMSPEWRTALASGRVVQGALAPPAEAEPVVYRLIVPVSVGHDVWGVIGFDT
                     LGPARRWQPTEIDALRTAAAAIGAALLRERDEAALRLSEQREHHAAGRWHILLDAVPA
                     LVWISADRNCTRITGNRMANQLLGIPPASNASLTAPEPDRRPPFRIFKDGVELTGDDL
                     PMQRAAQDGVEFRDYEEEIVFPDGGRLTLLGNVVPLLGPGGRPDGAVAAFIDITDMKR
                     AAEEKQNLERQILQAQKLESLGVLAGGIAHDFNNLLTMVLGNANLARVGLPANSPASA
                     HLAEIEKASRRAAELCRQMLAYSGKGRFVVERLSLSAVVEDMAHMLEISVSKKAVLKY
                     DFAPDLPVVEADPTQLRQVIMNLIINASEAIGDRSGYIRVGTGQADLRSEDLAGALLG
                     EEARVGTYVYLEVEDTGCGMNAESLGRIFEPFYSTKYKGRGLGLSAVLGIVRAHSGVM
                     RVRSEEGRGTTFRILFPAVTGSMPAVSESPAGGDAWRGHGCALLADDEEGIRLVGRRI
                     LEQLGFKVITASNGREAVDCFRQQAGEFRCVLLDLTMPELDGVEALEMIREIKPDVPV
                     LLTSGYDEKDVQRRYGSAPLSGFIQKPFEANILRNKLRIALGDGAPAAGDRSP"
     gene            15707..>15946
                     /locus_tag="PK634_08840"
     CDS             15707..>15946
                     /locus_tag="PK634_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97880.1"
                     /translation="MKSKVSRWGLIGLLLGCWVATAGAGEVTVRFEFSADDVSLVAVD
                     GYQRLALADGSLPVDEVGAPAIPARYANILVPAGAE"
BASE COUNT         2638 a         5398 c         5378 g         2532 t
ORIGIN      
        1 gtatgggtca ccctcaggac aaacggcgcc tcgtagcagc cgaggtcgac gcacgcgccg
       61 acgagccggt tcgatccgtc caggtccttc gccgtggcgg tccacggcag gttcgtcccg
      121 gcgtcgacgc agggcgagtt gaaccggacg tggtaactgc cgtcgacaaa gtcagggtcg
      181 ttcgagatgc agccggtgcc gtaggggagc cccgtcagcg gggtcgtgca gcagtactcg
      241 tagcggccgg tcgtgttgaa ctgccagttg tccccgctcg tcgtcgccgt gttgccccag
      301 atgatgtcat tgaggttcgt cccgttctgg taggtgaaga cgccgccgcc ctgcttcgcg
      361 gtattgctga cgatcgtgca gttctcgacc gatcctccgc cggaggagat gcaaaggccg
      421 ccgccaaggc ccgtcccgct gctggccgag gcgtcgttgc ctcggaacag gcagttgcgc
      481 aggaggctgc cgtatcggac gtaggcgccg ccgccgtttg ccgtcacgac aaagttgctc
      541 tcgatccggc tgttccagat ctcgccgcca taccagaccc cggctccgcc gccgtcggca
      601 tgcgcctggt tgccggacaa cacgcagttg gagatcgtgc cgcccatcag cagcgcgccg
      661 ccaccgcccg tgtcgaaggg ccagttgccg ctgttccccg tgtgtccgaa ggtcagggtg
      721 aaaccggaca gaaccgtgcc ggtgctgacg taggcgcagc ggacggcgag cggaccgttt
      781 gagagagtgg tcgaatcaaa catgccggcg atggtggtga cgttcgggcc gttcatgctg
      841 cgaacggtga tgccgttcgt caggcagacg cggctgacca gcgttccgcc tggcgtcgcg
      901 cgccccccga ccgagtacat gccgtttgtc acaaggacca ggccgcccgc cccggccgcg
      961 tcgaccgcgg cttggattgt gtggaaggcg ttgctccacg ccgtgcccgg cccgttggcg
     1021 gggctgttcg tccagacgta aagggtggcg gcttgaacgg acccggcaac cagtaaaccg
     1081 gatatcaggg cgaccatacg tttcatgacg cctactcctt agttttcgag ctgcttcaac
     1141 atcccatgaa caagatgatt cgcggccatg cgcacaagag ccttgtcgta gcgccgcttc
     1201 ccaagtcgcg cgaggatggc cgcccgcgcc gtcacgaaga acgacacttg cgcccagagc
     1261 gagccgtacc acagcgcgaa cgcggtcggt gtcatgcgcg gacgaatggt ccggagcgcc
     1321 gggtggaacc actcgcgcgc gggcctgagg ccgtcctgga atgccttcag cgcatcgttc
     1381 agattctcgg tgagcgcgcg ccccaggatc tcgccgcacc aggaggagcg gccgtcgggc
     1441 gggatgaccg cgaagaaaca cgcgcggatc tttgcggcga tgcgggtggc cgttccggac
     1501 ggttcgctcg cgaagagggc ggggaaatcc atcttctccg tcaacgcgta acgaacgcac
     1561 tcggcgaaga gctgttcctt ggtgccgaag tggtggttcg gcatccggag agacacccgc
     1621 gccgcggtcg cgatgtcccg aaacccgacc ccgttgaacc cgcgcttcgc gaacaaccgc
     1681 cccgccgcct ccacgatgcg ccggcgcgtc acctccggct gacgggcctg tcgacctcgt
     1741 cctttagtca tacgggcgta taataacgta tgcgcagcac ggagcaagca cgatctcgtg
     1801 ggcgtgaagg ccgcgagcgg cgtcacgagg caagaagtag ctgggaggtt cctgtgcgct
     1861 ccctcctctg tcgggattgc ggcgggccgc tccagccctt ccgccttccg cttcagaaaa
     1921 ttcaacaact acagacttcg cgtgagcgcc ttatgtgcct gatgaggggg gggcctacct
     1981 ttttttgtgt tgaaccgagc cgccgtggca cccctgtagc ggctcggtga tgatgggacc
     2041 actttttacg gacaacaggg taacacctgt gcatccaccg agacggtgat cgcaccgttt
     2101 cggtgtaatc atgattccac tgcccgacgc cacgcggcgt cttcttgcgc gtcttgggca
     2161 tcagcccctg ccggtgcagc gtccgccgca ccgtctcggg gctggccggc aggaacagca
     2221 cccgccttaa ccactggctg atccgcttga ccccaaagga cgggttctgt tgcttaacct
     2281 cggtgatctt ctggcgcagg gcatgggcgg aacgcttcgg cggggtgcct ctgcgttggc
     2341 ctggcgccaa gccggattcc cctccggcct tgtagcgctt ggcccagtcg tataactggc
     2401 tgggatggat ccccacctcc cggcttaccg gcttggggga cagcccttct tccaggtaca
     2461 gctttacggc cttcagtcgg acttccagcg ggtacgccgg ccgcgatgcc ccatcggggg
     2521 gcatcggttc atcttcctct tcatcacgta acactccttc ccggctctca ccgaaccgta
     2581 gctttcggag tgttaccccg tttccctttt aaactggtcc tatcatctgg tcgctaggac
     2641 atctgcggtc cgaaggttct gaggcacggc gcctcggaca agaacgtcga gattcacgca
     2701 gtgttttttg ccgtttgcct cataccggcc gtaccagtac gagcttcgga gcgagccatc
     2761 tttgttgcgc ttgagctcaa gcgtcacttc aaaacccccc acccatgata ctgatgtgaa
     2821 gggtggctga aggtgggaaa ggagtcaagt gcgttgacgc actttcggac tcacttggcc
     2881 tgttaaaaaa atacgggttg aaaaattatt tacaactact tgctttggaa cgtattagat
     2941 ggcgtacctg gcaggagtcg aacctgcaac cttctgatcc gtagtcagat gctctgtcca
     3001 attgagctac aggtacgccg gaaaagaaac cggacggcgc accagccatt ctggcgcggc
     3061 actacatagc gcattccggc gtccacgtca atcccgcctc aaaactgaaa gtagatatac
     3121 ggcgcgctgt tgttgaccgc gagcagaacg atggccgcga ccagcatcga cagggcgatc
     3181 gtgcgcgcga cccagggcca ccggcggatg cggtcgttga gatccaggta ttcctgggcg
     3241 aactccacca gggcgaacgc gacgaagatg gcccagtagc cccacaccac ccacggcgtc
     3301 ggcgggccgg cctcggcgcg gaagccgaac atgatcttca gcatgcgccc gagaaccggg
     3361 acgttcgggg cgcggaagaa gacggcgctg aacatcgtgc agagataggt aaacgcgacc
     3421 gcgaagagaa cggagacgcc cagccggcgc gacgggttct tccagcccat gcgctttcgg
     3481 aatccgcgcc agaggtagta gagcgccagc agcacggcgt ggaatccgcc ccagaacacg
     3541 aagtgccagc tcgctccatg ccacaggccg cagagcagca gcacgatcat cccgttcagc
     3601 agcgcccggc ccccgccctt gcggaacccg ccgagcggat agaacaggta atcgcgcaac
     3661 cagctggtca ggctgatgtg ccagcggttc cagaaatccg cgatcgacga ggcgtacagc
     3721 ggccggcgga agttctcgac gatgcgcacg ccgaacagcc gcgcggaccc gatggcgatg
     3781 tcggagtacc cggcgaagtc gaagtagatc tgcagcgaga acgccatcag cgaggtccag
     3841 accgcgaagg tggagtagcc ttccggcgcg gcgaagacgg cgttcgccag gattgcgaac
     3901 cggtcggcga gcacgagctt gcggaagaag ccgaagatga tccgcgtcag ccccgcgccg
     3961 atatcctccc acgtcacgcg ctggtagacg agcaactgcg gcagcaggtg gttggcgcgc
     4021 tcgatcgggc cggcgacgag ctgcgggaag aaggtgacga acagcccaaa gttgatgagg
     4081 tcgcggcacg gctcggtctg ccgccggtac atctggatcg tgtagctcat cgtctggaag
     4141 gtgtagaagg aaatgcccac gggcaggatg atgcgcatgg gcgtccagtc gatctcgaag
     4201 cccagcagcc gcccgagccc gaccgtgttc gcgatgaaga aatcgccgta cttgaagaag
     4261 cccagcatgc cgaggttgcc ggcgacgctg cagccgagga tcaggcggcg caggcccggg
     4321 ttctgcgtcc gcgcgagccc gaggccgcag tagtaatcca gcagcgtgga gatggccagc
     4381 agggtcccgt agatcggggt ctcccagcag tagaacaggt agctcatcac gagcaggaag
     4441 atctgccgcc cccgcagcgg caggcgcagg tatacgcaga gcacggccac caggaacacc
     4501 gcgtatgtga agctgttaaa aagcatggcg gctcacgggc ttcccgtctc cagcgcggcg
     4561 atccgatcgc acaggagccc ggccaaacgc tcgtggttgg gcggcttgag gtgactgctg
     4621 gtcaggaact cgtcatccgg cagggcgtcg cgcaggtccc acagcggcag gccgcgcgct
     4681 tccgcctgcg gcgccaggtc ccggatgaag gcctcgtccc actgcgcccg ggcgtcatgc
     4741 gcaagggcgc ggcgcggctg ggcgacgatc accgcgggca cgcccgtttt cttcagcccg
     4801 tccaggtact cggccagcat gtcgcgggac ggcccgtcgt acgtgaactg gatgtccccg
     4861 ccttccgcgc gcggagcggc atctcgggaa gccaggcccg gcccttcagc tgccaggggc
     4921 ggtagttcac ggcgggcgcg tagaacgtgc tgtggatgcc cggcaggcgg atgtccagcc
     4981 aggaccacag gtattccttc agccggtaga gcgcgacgcg gtcgtacatg gcgtacgcga
     5041 gcccgtcctc gatgcggccg tggcgccgga ggaacgagcg cggcaggccc cgcaggatcg
     5101 cgggacgcga ggcgaggcgc gggaggtcgg tccggcagtg cagaaagccg cggttcgcgt
     5161 gctcgaggct gtagtcggcg aaaccggtga ccgccaggat tcgcgacggc gggtgcaatt
     5221 gcaggtagcg ggccaggagc acgtactcga tcgaggtcat cccgtcggac gaccagttca
     5281 gcacctgcca gtcggaccgg ccgcccacgc ggcgctcgtt cagcagcgcc tccagccgcg
     5341 ccggataggt ctgccggccc aggtactcgc ccgcgtaggc ctgcgagttc gagatcagca
     5401 cgacggtggc gcccttcggc ccgtgcccga catcgcggat gaactccggc cagccccgga
     5461 agcggaacgg gttgcggttc tcctccaggc tgccgggcac gtccggaagg aggcggcgcg
     5521 ccaggacttc gcagagcccc agcacgatcc cggcgatcag aatgacccac ggccagcgac
     5581 gtttcatgac ggcaaatgga aagccggtac gctaggggaa gccgccgcgc ttggcaagtc
     5641 ccgatgcttt acagccggga ggcctccgcg tataacaggc ccgcgttgaa gcggatcgaa
     5701 tgaatatccc ccccgccagt tcggagaccc gacgcgcccg actggtcggc gccgccttcc
     5761 tcctgctgtt cgccgccgtg ttcctccaga acgcctgggt ctgcgacgac gcctacatca
     5821 cgttccggtc catcgacaac ttcctgcacg gccacggatt gcgatggaac gtcgcggagc
     5881 gggtccagtc gttcacccat ccgctgtgga tgctgctcat gacggcggcg gcttccgtca
     5941 cccgcgagtt cttcttcacg gccatcggcg tgtccctggt cctgtgcctc gcggcccttg
     6001 ctctcctgcg gggcggcccc gtactcgcgg cgttgctggt cagttccaag gccttcatgg
     6061 actactccag ctccgggctg gaaaacgcgc tttcgtacct gctcctagcg ctctttctcc
     6121 gggagttcct gcaggacgcg cccggcgagg ggccccgcga ccggcgccgc atcggccggc
     6181 tcgttctcta cgcgaccctc gcctacctga accggcacga cgcgatcctg ctgtacctgc
     6241 cggcgcttct cgtgatcgtg gcggccgggg tccgccggca cggcgccaca gcccttgtcc
     6301 cggtcctcgc cggcggcctg ccggccgtcc tgtggcatgc cttctcggtg ctctactacg
     6361 ggttcttctt cccgaacacc tactacgcca agctctcggg cgcgggcgtg gacgcgggag
     6421 tcctgcaccg gcagggctgg gcctacctcg ccaacagcct gcgtttcgac ccggtcacgc
     6481 tggccgtcac ggcgctcggc ctgccggcct ttctcctgtc cgatcgccgg aaggcggcgg
     6541 gcgcctgcgt cgccgccggg ctgttgctgt acttcgcgta cgtgatatgc atcggcggcg
     6601 atttcatgag cggacggttc ctcgccctgc ccttcctggc gggcgcgctg ctggccgcgc
     6661 gaacggcgtc ggcgcgccgc ctggccggac cggcgctggc cctgctcgcg ctgtaccacg
     6721 tcgtcgcgcc gcgcgcgccg atcaagacca cccgccacta cgcgcggtgg gacgccgacg
     6781 agcagcacgg catatccgac gagcgggcct actaccagga cggcgcgtcg ctgatgttct
     6841 actccccggc gcggccgccc ccggacaacg cctacacgcg gctggcgcgg caggcgaagg
     6901 gcgcgccgga caaggtgttc atctcggaga ccatcggctt cgcgggatac ctgatgggcc
     6961 cggagaagtt catcatcgac aagctgggcc tgaccgaccc cctgctggcg cgcctgccga
     7021 tctcgcggcg cagcctggcc aatttccaga tcggccattt cgcgcggcaa ctgccggcgg
     7081 gctatttcga atcctgcgcg gacggcgtca accggatcga ggacccgggc gtccgggcct
     7141 actacgacgg cctgcaactc atcacccgcg ccccgctgtt cgcccccggc cgctggaaag
     7201 cgatcgcgga gtacaacctt ggcgcgaagc gtcgctacga tcggccctac ctggccgact
     7261 gaccggcccg ccgtcttttc ccgttgacgg gcttggctta aacgtataat tatccgcgta
     7321 agattgtttc aaagaacgga ggatacatca tgcgcctgcc cctttgcctg atggtatgtc
     7381 tgtgcagctt gagcgccgcc gaggccgcgg tcaccaacta tgtcgaaacc ttcaacgaca
     7441 cgggcgacca ggagcatcac tggatgtact ggggccaacc cacgggcggc ggcgaggtcg
     7501 ccgaccagtc cgtcgtctgg atggccaacg gcggagcggg cgggacccct cgcgtgaccg
     7561 tggacatgga gaacctggac tacaacgcga acggctactg gctccactac cttaactcgc
     7621 ccgacgtcgg cggcacgaga accaccgacc aggaactgaa cttcaacgac cacatcgtca
     7681 ccgccgcgtt caactcgcac aacatcgcgg atcccttcaa cggcgggtcg gtcttcttct
     7741 tcctcggaca gtacatcgac ggcggcaact cggcgtggta ctacttcaac caggcgttca
     7801 ccgtcaacgt gacccaggac gactggtcgc aggccacgag catcggcatc acgggcgccg
     7861 aatcggaatg gacgctcctg tccggcaacg cctcgatctc ggtcacgaac ctgttcaaca
     7921 acccgcagca gtacgggttc gtgatcgtcg gagcgggcag ccagccgacg tacaacctgt
     7981 ccatggacaa cttcagcgcg gcgcaggtca tccccgagcc gggcgtgctc gcgctcctgg
     8041 gcgcgggcct cgcgctgctc cacctgaaac gggcgcggtc gccgggccgt tgaggcttcc
     8101 ttcttgcttt tcagaccggc ccggccatca tggcccggtc atgggcgttc gggaaaaatt
     8161 cttctcggcg gacacgtacc gcaacgtcga gggcatcctc gggatcgtaa aggacggcct
     8221 ctgccaccgg tgcggcgcct gcatcggggt ctgccccgtc ggcaccttcg ggatcgacga
     8281 gcacgcgtac ccgaagcagg tggacaagtg catcgagtgc aacatctgcg tgcgcgtgtg
     8341 ccccggcctg gcggtggact atcccgcgct gggcgaggcg ctgttcccgg gccgctacga
     8401 gttcgggagc ctgatgggcc cggtcctctc gacgtgggtg gcccacgccg gcgagccgga
     8461 catccgccgg cggggcgcca gcggcggcgt gatcacgcaa ctgctcgtcc actggctgga
     8521 gaccggccgg atcaagggcg ccctcgtcac ggtggaggac ccggaggaac cgtcccgcgg
     8581 caagggcatc atcgcccgca cgcgcgagga catcctgcgc tccgcccagt cccgctactg
     8641 cacagcgcct tcgttctccg cgctttacga catccgcgac gaggaaggcc cgttcgccgt
     8701 cgtcggcctg ccgtgccaga tccacgcgct gcggaagcgg cagcggatgg acccgaggtg
     8761 gaagaaacgg atcccgatgg ccatcggcct gctgtgccat tacaacctgc ccttcgaatc
     8821 cacgcgggcc tccggccgca tgctcgcgcc gaaaggcgag cggctggccc acgcgaattt
     8881 ccgccagcgc gacgagcggg gctggccgca caacacgctc gaactgacct tcacggacgg
     8941 ctcgaaatgg cggtcgccct acggcccggc ccagatcttc aacatcgtct cccgcgtttc
     9001 cccgctgggc cgctgcctga tgtgcctcga cgcggcggcg gagttctccg atttttccat
     9061 cggcgacccg tggatccgcg acgagaaggg gctctggaaa tacgacgagc ccgggggctg
     9121 gtcctcggtg ctcgtccgca cgccggaggc cgagcagtgg ctgcgcgaag cggagcaggc
     9181 gggcaagctc acgatgaagc ccgtgccgcc ggccgaggtc gagcggggcc agcacgcgat
     9241 gatgatcgag aagaagcagc ggacggcgtt ccggctgcgg ctgcggaagc ggttcggccg
     9301 gccggtgccg gactatccca tgcccctgcc cggcacgtcc gccgaacaga agcgcaagga
     9361 gatggccttc tggctcaccc ggatcctccc gatgttcccc tggctcgccc ggctggcgct
     9421 gcgcatcggc ctctcgccgt tcggcggcta cttcgtgcgc cgccgcatgc gggcccgcca
     9481 gcgcgccgcc gcatccggaa agtacctggt cagcgcctcc gacttcgggg acgcgcggga
     9541 gaggaagtca ggctgacgcc gccgcttcgc gccggctcac cttctgcacg ctgatgcact
     9601 tcacgggaca cacctcgagg caggcgttgc agcgaatgca gtcttcctgg tgaatccagt
     9661 agaggaacgg cggcgtaccc gggaggagat gctcgtcgcg ccggtcccag cccgtgatgc
     9721 gaccctctcc gtctttccgc agggcgcgcg ccttgacgat gcagtcgggc cgcggcttga
     9781 cctcgacgca ctggtcgcag tagatgcagc ggtccatgtc gatctcgtac ttgtagtggc
     9841 acaggtaaca ccggcccgcc tcctcccgcg cgcggtccgc cggcaggccg gtctccacct
     9901 ccgcctccag cttccgcgcg tcgaccggaa gtcccggcat tggctgccgg ggcacggcgt
     9961 ccatggcccg cgtgcgcgca acctcacgac cggactcgat gcggacgaca tcctccacgc
    10021 gggatccgcc catcagcgcg gcgtccacct cgcgggtaat ttgcctggca tgaccgatgg
    10081 cctcgatcaa cgtcgtggcg ccggtggcgt agtcgccggc caggaacagc ttcggcgaag
    10141 ccaaggccgg ggcccaactc cggtccgcct gctggccggt ggccagcagg acgaggtccg
    10201 cctcgaccgc gaattcgctg ccggggattt ccacggggcg gcgccggccg tcggcgtcgg
    10261 gctcgccggg ccgggtgcgg atgaagcgga cggaccggac gccgcccgcg tcgccgtcga
    10321 acgccacggg ggcgacctgc gtttccaggg ggatgccctc ctccgccatc tcctccagtt
    10381 cacccggcgt gacgcgcatg tcgtccgcgc tccgccggta gaacaccccg actttctccg
    10441 cgcccaggcg cagcgcgacg cgcgcgcagt cgatcgcggt gaacccgccg ccgatgaccg
    10501 cgacgcggcg gccgatgtcc cgccggccga gctcgttgac ggagagaaga aactccaggc
    10561 cgtgctcgac gcccttccgc gacacgcccg gccctttcgg aatattcggc cgcagggtcc
    10621 cggcggccag caccaccgcg tcgtgatcgg ccagcagttg ctccaccgac acatcgccgc
    10681 cgacgttcac gccgcacgcg atccggacgc cggcggcctc gacctggcgg atttcggaat
    10741 ccacgacatc gcggggcagg cggaacgcgg ggatgccctg tcgcatcatg ccgcccggcc
    10801 gttcgtgccg ctcgtagacg acgacctcgt gccccagcct gaccagctgc cgcgcgacgg
    10861 tcaggcccgc gaccccggcg ccgacgacgg ccaccttctt cccggtcgcg ggaaagaccg
    10921 gcgacaagac gacggcttcc cgcggggcgc ggtggccggc ggccgcgcgc ttggagaagc
    10981 agatggcgac cggctcgccg aggccgggcc agccgtggcg gcagggcggc tcgcaggggc
    11041 gggcgcagac acggccgagg acgccgggga agacattgtc ggccaggttg atgcggtagg
    11101 ccccctcgaa gtcgccctgc gagacggccg cgagatagcc ggggatatcg gtctgcgccg
    11161 ggcacgccgc ctggcagggg atgttccttt ccagccagtg aaagtcgcgg ggctcccggg
    11221 cgacaccttc ggcccgtgaa aggggcgttt cgttcatcac cggaggcgct cgacccgggc
    11281 ggggcagccg tacgcgatgg cccattcggg caggtccttg ttcaccagcg agtgcgcgcc
    11341 gatgaccgcg ccccgcccga tggtcacgcc gtccaggatg gcggtgtgcg agccgatcca
    11401 cacgtcgtcg ccgatggtca cgccgccgcg gctggtgaag ccctgcttgc ggatgggcac
    11461 ggtcttgtcc gcgtaattgt gattcccgcc cgcggcgatg taggtgtagg ccgcgacgag
    11521 cacgtccttc ccgatcgcga gccggctgtc cgtggccacg atgcagttgc agccgatatc
    11581 ggtcccgtcg ccgatggtca gcgattcgcc gcggcagcgg acgatgctgt tgcggccgac
    11641 gagcgcctcg tcgccgagcg tgatcgaggc cccggcgccg cgcgcgtcca ccaccgagtt
    11701 gtcgtccagc acgacgcggt ccccgacgcg gatgcgggcg aagccccgga tggtcacgtt
    11761 ccggccgatg atgagttcgc ggccggccgc ggcgaacagg cgacggtaga gcttgcggcg
    11821 cagccagatg ccgagcgcgc cggggcagcc cgagaggagg ccggtgacca gttcgtgcac
    11881 gaagagcgtc cagagcgagg tcccgccggt ggtcagcagc gcgtagcgcc ggagcgcgga
    11941 ccggcccgga tcatggatcg ccgcctgggc ttccagtttc agtttgttgt tggattcgtt
    12001 cataatcaaa ccgttcatgc gggtaggtca ggtagctgac caggcccgcc agtcccaccg
    12061 cgagcagctt gaaccgcagg aacaggccca tggccagcgt ggccgcgggc tccatgccca
    12121 ccagcctaaa atagaagaca taggcccctt ccgcaatacc caggctgccg agcgtaatgg
    12181 gaatggaccc ggcgagcatg gcggtggcca gcaggctcat gatgccgatg aaatccggct
    12241 ccacgccaaa ggtgcggtag gccaggtaca cgttgaccca ggtcagcgcg tagaacagca
    12301 cggtcaatcc gaacacggcg agcgcggcgc gccggttctg cttgagcagg cgcatggtga
    12361 gggccatctt ctcggcgatc ttctccgact tcgcgcggaa ccagaggagg gccttcatgc
    12421 cccgcccgag cagcgagcgg cccggcgcgc cggccgcgcg gagggcctgg cgctcgatca
    12481 gacggttgag gaagcgcagc agcccctgcc cccagaaagc cagcgcggcg aagaaaagca
    12541 gcaggcccgc cgcgcccgcc gccgggacga acaccaccgg ccgcgcatag agcccgggct
    12601 tcagcagcgg catgccgatg gccaggacca gcaggtacag gctgccggtg aaccgttcca
    12661 tgaagacgct gatggccgcg tgggtctggt tgccgatccg ccgtcccgcg taatacgagc
    12721 ggaccacgtc cccgccgatg ttggacggca ggaggttcga gaagtagtag ccgatgaggt
    12781 agttgcgcat caggaagccg aacccgagcc ggtcgccgta caggttgatc aggacgcgcc
    12841 acttcaggca gctggtggaa atctgggcgg ccgcgatgac gaacgacagg gtgaaataca
    12901 ggggatccac gcgcttcagc atcgccagca ggtcctgccg gccggcaaac gcgaagagca
    12961 cggcgaagaa tacaagactg accgcggcct tgacgagcag cgtgcggcgt ttcagcggct
    13021 ggccccgact ggacatgggt tcactatatc agagcggtcc ctcgaagcaa cgccggcgcc
    13081 ggagcgccta tccaaaaaaa cgcgccgggg gtacggatga tccccggaac ctcatcttgg
    13141 acagcgcgct gatatcggac ggggcgcccg cccggcggcc cgcgcgtttg cacacgcgcg
    13201 tcggtctgct ttgcctggtt gtccttgcgt ccccggcggc atccgcgagg gccgccccgg
    13261 gcttgccgga gggcgccggc tccttcccct gggtccccgt cctcctgctg ctcctggccg
    13321 tcgtgggcgg gacgatcgcc tgggtggctc ggcgggaact ccggcggcag gcgcgcgacc
    13381 tgcaccgggc ccggcgcgag atggaggccc ggggccgcat cctggccggc gtggccgaga
    13441 cgggcgcgct gctgctcggc gaatcccagg tggaccgggg catccggcgg gcgctcgaga
    13501 tcctgggacg ggccgccggc gtggaccggg ttttcgtgac ggaagagctg gcgcccgcgg
    13561 ggccgggtcg ctcgatcgcc ggcatccgct gggaatggcg ggcctcgccg gcccttgccg
    13621 gcgcggggcc gagcgcggag atgagcccgg aatggcggac cgccctggct tccggccgcg
    13681 tcgtgcaggg cgcgctggcg cccccggcgg aggccgagcc ggtggtctat cgcctgatcg
    13741 tgccggtcag cgtcggccac gatgtctggg gggtgatcgg cttcgacacg ctgggcccgg
    13801 cgcgccgctg gcagccgacg gagatcgacg ccctgcgcac ggcggccgcc gccatcggcg
    13861 cggcgctgct gcgggagcgc gacgaggccg cgctgcggct ctccgagcag cgcgagcacc
    13921 acgccgccgg ccgctggcat atcctgctcg acgcggtgcc cgccctggtc tggatctccg
    13981 cggaccggaa ctgcacgcgc atcaccggga accggatggc gaaccagctg ctgggcattc
    14041 cgccggcgtc caacgcgtcg ctcaccgccc ccgagccgga ccgccggccg cccttccgga
    14101 tcttcaagga cggcgtcgaa ctcacggggg acgacctgcc catgcagcgg gccgcgcagg
    14161 acggcgtgga gttcagggac tacgaggagg aaatcgtatt cccggacggc ggccggctca
    14221 ccctcctggg caacgtggtt ccgctgctcg gcccgggcgg gcggcccgac ggggccgtgg
    14281 ccgcgttcat cgacatcacg gacatgaaac gcgccgcgga agaaaagcag aacctggagc
    14341 gccagatcct ccaggcccag aaactggaga gcctcggcgt cctggcgggc ggcatcgccc
    14401 atgatttcaa caacctgctg accatggtgc tcggcaacgc gaacctggcc cgggtggggc
    14461 tgccggccaa ctcacccgca tccgcgcacc tcgccgagat cgaaaaagcc agccggcggg
    14521 cggccgaact ctgccgccag atgctcgcct attccggcaa ggggcggttc gtcgtcgagc
    14581 gcctcagcct gtccgcggtc gtcgaggaca tggcccacat gctggagatc tccgtgtcca
    14641 agaaggccgt cctgaagtac gactttgccc ccgatctgcc cgtcgtcgag gcggacccca
    14701 cccaactccg gcaggtgatc atgaacctga tcatcaacgc ctccgaggcc atcggcgacc
    14761 gcagcggcta catccgggtg ggcaccggcc aggcggacct ccggagcgag gacctggccg
    14821 gggccctgct cggcgaggaa gcgcgggtcg gaacctacgt atacctggaa gtggaggaca
    14881 cgggttgcgg aatgaacgcc gagagcctcg gccggatctt cgagccgttt tattcgacga
    14941 aatacaaagg ccggggactc ggcctttccg ccgtcctggg catcgtgcgc gcccactccg
    15001 gcgtgatgcg ggtgcgcagc gaggaagggc ggggaaccac gtttcggatc ctcttccccg
    15061 cggtcacggg ctccatgccc gccgtttcgg agtcgccggc cggcggcgac gcatggcgcg
    15121 gacacgggtg cgcgctgctg gcggacgacg aagaaggcat ccggcttgtc ggacgccgca
    15181 tcctggagca gttgggcttc aaggtgatca cggcctcgaa cggacgcgag gcggtcgact
    15241 gcttccggca gcaggccggg gagttccgct gcgtcctgct ggacctcacg atgccggaac
    15301 tggacggggt ggaagcgctc gagatgatcc gcgagatcaa gccggacgtg cccgtcctct
    15361 taacgagcgg ctacgacgag aaggacgtcc agcgccgcta cggcagcgcg cccctctccg
    15421 gattcataca gaaacctttc gaggccaata tattgcgcaa caagcttcgc atcgcgctcg
    15481 gggacggagc tccggcggcc ggggatcgat cgccttaatc ttttcgcccg tatttttcct
    15541 tcccccgctc cggatcaaag gagtactttg aaggtttgaa ctcgtgtcgt tcccgccgtt
    15601 cgggacgacc gcgaaaagac gtggcgctgg gcatgtcata caccggatcg caggggcgcg
    15661 gagccgggtg accgcgacgg aatttacaca gggagaactg atcacgatga aatccaaggt
    15721 ttcgcggtgg gggctgatcg ggttgttgct cggatgttgg gtggctacgg cgggcgcggg
    15781 cgaggtcacg gtccgcttcg aattttcggc ggacgacgtg tccctggtcg ccgtggacgg
    15841 gtaccagcgc ctggcgctgg cggacgggag cctgccggtg gacgaggtgg gcgcgccggc
    15901 gatcccggcc cgctacgcga acatcctggt gccggcgggc gcagag
//
LOCUS       DAOOTK010000070        15784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2529_length_15784_cov_6.370462, whole genome shotgun sequence.
ACCESSION   DAOOTK010000070 DAOOTK010000000
VERSION     DAOOTK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_15784_cov_6.370462"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="PK634_08845"
     CDS             complement(<1..1909)
                     /locus_tag="PK634_08845"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOW97881.1"
                     /translation="MNIKEQKHVDVAIGGKTIRFEAGILAQQAAGSATCRLGDTVVFS
                     AVTTGKSKREGIDFFPLQVEFREKTYAAGRFPGGYFKREARPSEKEILTARMTDRPIR
                     PLFPEGFFDEVQINNAMLCADGENESDIPCINAASAALTLSELPFRGPVGAVRVGRID
                     GQFVLNPTHSDLAKSDLNLVYVASRDLPLMIEGTAQEISDEDIVAAMKFGHEAVVKLV
                     DAQHELRRMFGLPEKKIQDNPVPEDILQKSRELAAADIAQALDIDDKQTRGSRLAEIG
                     DALKAKLLEWKPDLDDVTWKRTFDTLEIECVRSLVLDKGRRIGGRAFDQLRPLQAQVG
                     LLPRTHGSALFSRGETQALATVTLGTMSDAQEMDALTGGPDEKRFMLHYNFPPYSVGE
                     VGRLGGVGRREIGHGALAERSISPMMPKDYPYTVRVVSEIMGSNGSSSMASICAGTLA
                     LMDAGVPLTKPVAGISIGLFTAPGKARLVTDILGAEDHCGDMDFKVAGTRDGITGFQV
                     DLKIPGLTWELVAGAFAQAKAARTKILDHMAGAIDKPRPDLSPYAPRITVLKINPEKI
                     GALIGPGGKNIKKLVEESGCEINIEDDGTVNIYSVSEEGMKIAKDAIAGMTAEAEIGR
                     IYRGKVVTIKDF"
     gene            complement(2019..2282)
                     /gene="rpsO"
                     /locus_tag="PK634_08850"
     CDS             complement(2019..2282)
                     /gene="rpsO"
                     /locus_tag="PK634_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOW97882.1"
                     /translation="MASAIKAEIKQEYQLHDKDTGSAEVQVALLSKRIKDLTGHLQSH
                     AKDHSSRRGLIVMVSKRRRLLDYLMRTHEDRYKTLVERLGLRR"
     gene            complement(2404..3390)
                     /locus_tag="PK634_08855"
     CDS             complement(2404..3390)
                     /locus_tag="PK634_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOW97883.1"
                     /translation="MPSPRVLVVKLSSLGDLFHALPAVRILREGLGAKVDWVTQTEYA
                     ELVRCFTDVDRVIPFPRRRFPAGGWAFFRALRESSYDMVLDFQGLLKSAIVARLARSR
                     RRIGPASGREGSPRLYSDRAGPPDRSRHAVEQGLDLVRHLGLPVSPPAFPVRFPPADI
                     PLPGPRVALVPCSRWPTKNWPPDLFYDVARGLQAQGASVFLFGSPEDRATCAAIEKGL
                     SGNILDLCGRTSLVELGGWMAGMDLVISVDSGPMHIAAALGRPVLAIFGPTDPARTGP
                     YGPMHRVLQADDCPCRPCYKRTCEREDLACLRHITPDLVLRNAAEMLAPPAP"
     gene            complement(3399..4355)
                     /gene="argF"
                     /locus_tag="PK634_08860"
     CDS             complement(3399..4355)
                     /gene="argF"
                     /locus_tag="PK634_08860"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOW97884.1"
                     /translation="MHNLITLKEWTPAQILDVIALAREIKKNPARFAKSMEGKTLLMI
                     FEKPSLRTRVSFETGMTQLGGHAIFYDTSTSPLGAGKESVYDTVKTVSRYVDMIMARL
                     FEHAKIEEMAREATVPVINALTNFAHPCQILGDLMTIQEKKKTLKGLILAYLGDGNNN
                     VTHSLLYGCAKMGIHIRVGCPAGKEYSPDAGVVKDAAQVARKTGGTVKITHDPREAIQ
                     DADVVYTDSWMSYHVPPHKLDERVKMFTPFQVNAALMKRARKDAIFMNCLPALRGYEQ
                     TAEVIDGPQSVVFDEAENRLHIQKAIMLKLMEQRKRGVGEKG"
     gene            complement(4606..5862)
                     /locus_tag="PK634_08865"
     CDS             complement(4606..5862)
                     /locus_tag="PK634_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOW97885.1"
                     /translation="MLFLKGHPAAGGIARGRLMFLDQPTTAGAGNAPARTLEDFRRAL
                     RQTEIQLERLQQQMEQRLEDAAALIFSAHLLMLKGDAFSGAMIRAIKGGAPPAAAEGV
                     PPDLTPETAAASGERFRVMANMNLLSDLLAVREMKAEGVGLYRAEFPFIVRHDFPSEE
                     EQCRIYRRILDELSGQEVVFHTLDIGGDKMLSYYPTPGEANPFLGLRAIRFSLRNQAV
                     FSQQIRALLRAGSERELSILFPMIASLDEWLAVRSIVQACVDALAREGIPHQAAPRLG
                     AMIEVSAAMEIAEELAAHADFLSIGTNDLVQYLLAVDRTNEQISDLYQSHHPAVLRAL
                     HRVAQAALRRGRPVSVCGDMAADRRMIPFLAGIGIRTFSVNPRSRPACQLVLRGLDLR
                     RAEEQARGMLKAGTLREVEALMAPPE"
     gene            complement(5850..6272)
                     /locus_tag="PK634_08870"
     CDS             complement(5850..6272)
                     /locus_tag="PK634_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOW97886.1"
                     /translation="MSRWKKDNVELICSVAELGALFERRRRLEDFLATIVSAMACHMR
                     AAVCSIYLYNEARGDLVLAANQGLRPGSVGRVRLALGEGITGLAVEEFRPICEGRAAR
                     HPHFKPVPDLDEERFEAFLAVPILRGVPSRRRNPECCS"
     gene            6388..7419
                     /locus_tag="PK634_08875"
     CDS             6388..7419
                     /locus_tag="PK634_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOW97887.1"
                     /translation="MPRTTIKDIAKECGVSLSTVSLVLNNNPRISAKTRETVLASVEK
                     HGYQPNASARNLASRTSRTICVAVPALSHVFADVYFGEIVSGIYDHASEAGYKVILDL
                     ANPKFVESKEYLKVLKSRRADGMLFISSSVHDTYLTDFEGGALPFLLVNHFFPGRDLN
                     FIAADYEDTARQAADHLINLGHTRLGLISGTNTHTGLALRDAFLRHCRSRGLKEEDVV
                     WLDSMRGKEWSQEGGFDAASELLTQHPATTAIMCGNDRLAIGVMRHLRMRRLRIPEDI
                     SVAGVDDIPESTYTNPGLTTVRHDLYKMGRTACERLLALFRAEIAECREILPVQLIAR
                     ESTGPARPA"
     gene            7538..8005
                     /locus_tag="PK634_08880"
     CDS             7538..8005
                     /locus_tag="PK634_08880"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOW97888.1"
                     /translation="MRVTWLIVDGYSLLHRFDPAGARRKGGLQAARQKLVRHVEDLAA
                     GRYDRVTIVFDGKESGGGEGYESPHVEILFSPVGRTADTVIERLVHDAPEPEAILVVS
                     SDRLERQTVSGRGAQTVGCGDFLAEGEQRQRHRPALSSRHPRPTLGDFFPDRA"
     gene            8083..>15784
                     /locus_tag="PK634_08885"
     CDS             8083..>15784
                     /locus_tag="PK634_08885"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOW97889.1"
                     /translation="MNGLRRGLCLGFGLILIAGAARAATLLVRTNSPSPAPPYADWDS
                     AAHTIQDAINEAGANDTVLVTNGVYDAGYTYMPGYTLACRVVITNGVQVISVNGPAET
                     VIKGEGALDSGAMRCAFLSEGATLSGFTLTNGFTRTAGYYYFDMSGAGAHILNSGTIT
                     NCVITGNTASYHAGGVMCHNGGLVVDSIVCGNLAHDNAGGGVYLYHNGTVSNCQVYDN
                     TAWWNGGGVEVSYTGLVTHSSLFANRAQNGDGGGVDLNYGGTVRDCLIYSNRSTGATE
                     YDGGGGAQCFFGGTITNSAVYGNSAVTRGGGVKIVFGGELVNCSIFDNTAGDGGGFYG
                     DYYTSNKNCVIYHNRALVGPNWSTNGSGNYFWANCLTPLPAGPGGGNLTNAPLLAGIR
                     NWHLLTNSPCVDAGSNVFATGIVLDLDREARIAGAAVDIGCDERTPAGLAGPLTALIR
                     AAATQVVVNIPLTFYSDVSGKVSRIEWTIQTESGPRATTNLFSEAQAWSVTGRFPVVL
                     AAFNNDSHAAVTAVVEVLAGFTNYVSELSPSPVPPYTSEATAATSLYEAVQVLPPGGV
                     TLMYYGNYPEAHEIIFDKPATVIGTFGPAATRISGLGAHRVIRISSVDAQLHGLMITD
                     GAAGAEPGGGVLMETAGVLSNCWIIGNVSSNDGAGVYLNAGGAMFDCLIASNRGVAWG
                     CDGGGAYFNRGGTSRGCRYEGNFAVGYGGGAYFNRGGTADDAAFLSNLSSNGGGGAYC
                     NYGGSMCNAAFLGNASYSGGGLYLYYGGRAMNAHVASNTAEHSGGGLYVYGNASPTCS
                     HATVQGNIAGVRGGGVYANSGGNILNSIVYYNRAPIGENFFNDWSGYRYNTCCMTPAP
                     ASGGYNILTNAPLFAGALNPHLLSNSPCLNIGSNAFADGIAADVDGEPRTNGVVDLGC
                     DEFWAEGMTGTLSVAILAPYTNAVAGTPLPFEAHIEGKANSYEWRFGDAGATNGFTNL
                     LALTYTWPSAGAYPVVLEAFNESGSAAATVTVTLVEGFTNYVDLANPTPASPFTNWAT
                     AANTIQEAVDAAAAGGLVLITNGDYNPAATIALSKPVRLQSVNGPDAVTVSGQGARRV
                     FNASAPGVSLDGLTITNGFTADNGGGILCGAGVSITNCRIVNCQAANGAGVYFNGGGA
                     LFNSHLQYNRADTAGGGAYCAIGGLAQACSFFSNVTVSTSGGGGGGAYVNTLGTFLDC
                     VFTNNLSHYRGGAGYGYYGGLFSNCLVARNQAKGWSSSVAGGGLYLSYGGLAQACTIS
                     NNVSDETGGGAYLYYGGAVEDCLVSTNWAEDGGGGVYINSIGTVRRSRIEKNAARWDQ
                     GGGVYFYRGGMAEACVFAENRSLSTWQGGGGAYADRAALVLTNCQFWGNEAYSGGGLY
                     ISEYGSAVLCSFWSNRATQGGGAYLNNGGRLHRCGFTNNVANQGGGLYVFDAGAASNS
                     MFIGNTAMDGGGAHLVNGGDLYNSFFAGNVASNQGGGVALASWGEYSTVAHCTIVGNT
                     CYLQGGGLWSDLGGTVRNTILRDNLAAFGDNWFHETGAAPTYEHCCVEPDPGGTGNLT
                     NAPLLAGRLNPHLLTNSPCVDAGDNAWAIGTDIDLEPRIAGPAADIGCDEMDPTNLVS
                     EIRGGIYAPGTNVTVQTPLTFYSDVQGQVRWMVWNVNDPDAPDGLAQFTNVPSVTYAW
                     NTPGDYQIMLQAFNGIWYGDGFVTVHVTEAQTNYVAPGGAHVWPFASWANASTSITEA
                     VSAAAYGGVVVVSNGVYREPAGILVDKPLVIRSVNGWDHTTLDGEGAHRGFVLFETNV
                     LVEGFTFSNGLAHADMFGGGGGVVLISGGTVRHCRFLNCSADQYGGGVLCYYGGSVQN
                     CWFTGNTAMFGGGALCYYGGEVINSVLVRNQALAQGGGVYCEGGGGVYNSIVYHNLGP
                     MGPNWLNVAGGITYENVCTQPLPPGPGHLTNAPGLAGLNNPHLLTNSPCFNAGSNVHA
                     AAIAADCDGETRVLDGRVEIGADEIYPPGLTGALAVAIAGDAFATGPGFPLQFWALVD
                     GKPAHTLWTIDAGGFGTAVVSDALTVSYSWSVTGSYPVVLSAYNNTDYAAATVTVTVA
                     DGTIRYVDKAGAHNPPFLTWADAAHSISAAVAVAVAGDTILITNGVYAEGTELIVNKP
                     LRLVGVNGPEVTVVDGANSHRVFRLEDPLAVLEGLGITQGSAAGSPGGEGGGVVLNGH
                     GQVVNCHIYSNQSPGHAGGVLLYGGGSVTGCVIRHNTAEEGAGATLFNGGLLAGSRLW
                     ANQATTRGGGALLMMGGMVSNCLIQQNNANMEGGGVAFYEGGTCAASLISSNLSSEGG
                     GAYFYLDGTLTHCVLQTNFAWNMGGGVRTYQGGLIRNCLFYHNAADPQWGFGSGGGVF
                     LRLGGLVENCTVVENYASVGRGGGILCNSGGTVLNSIVYSNHAENIANPEQSAFSYCC
                     AEPAPAGLVNFTNNPRFESWTAWDYRLASNSPCVEQGLNQDWMTNAVDLDATARLKHL
                     YADIGAYESVWTNVDTDGEGLVVGIERSRFGTSDFLTDTDGDGQDDWEEGMITGTSPT
                     NGALYFRGLIGPDAAGRSVVRWQGITNRV"
BASE COUNT         2555 a         5431 c         5293 g         2505 t
ORIGIN      
        1 cgaaatcctt gatcgttaca acctttccgc ggtagatccg cccgatctca gcttccgcag
       61 tcatccctgc aatggcgtcc tttgcgatct tcatcccctc ttcggagacc gagtagatat
      121 tgactgtgcc atcgtcctcg atattaatct cgcaaccgga ctcctcgacg agcttcttga
      181 tgttcttgcc gccggggccg atcagggcgc cgatcttctc ggggttgatc ttgagcacgg
      241 tgatccgcgg cgcgtacggg gacaggtccg gccgcggctt gtcgatcgcg ccggccatgt
      301 ggtccaggat cttcgtccgc gcggccttgg cctgcgcgaa ggcgcccgcg accagctccc
      361 acgtcaggcc ggggatcttc aggtccacct ggaagcccgt gatgccgtcc cgcgtgccgg
      421 cgaccttgaa gtccatgtcg ccgcagtggt cctcggcgcc gaggatatcg gtgacgagcc
      481 gggccttgcc cggggccgtg aacaggccga tcgagatgcc ggcgaccggc ttggtcagcg
      541 gcacgcccgc gtccatcagc gccagggtgc cggcgcagat gctggccatg gaggaggagc
      601 cgttggagcc catgatctcg gacacgacgc gcaccgtgta cgggtagtcc ttgggcatca
      661 tcggggagat ggaccgctcg gcgagggcgc cgtggccgat ctcccgacgg ccgacgccgc
      721 cgaggcggcc gacctcgccc acgctgtagg gcgggaagtt gtagtgcagc atgaaccgct
      781 tctcgtccgg gccgcccgtc agcgcgtcca tctcctgggc gtccgacatc gtgcccagcg
      841 tgacggtggc cagggcctgg gtctcgccgc ggctgaagag ggccgagccg tgggtccgcg
      901 ggagcaggcc cacctgcgcc tgcagggggc gcaactggtc gaaggcgcgg ccgccgatgc
      961 ggcggccctt gtcgagcacc agcgaccgga cgcactcgat ctccagggtg tcgaacgtgc
     1021 gtttccaggt cacgtcgtcc aggtccggct tccactcgag aagcttggcc ttcagggcgt
     1081 cgccgatctc ggccaggcgg ctgccgcggg tctgcttgtc gtcgatgtcc agcgcctggg
     1141 cgatgtccgc ggccgcgagt tcgcgggact tctgcaggat atcctccggc acggggttgt
     1201 cctggatctt cttctcgggc agcccgaaca tccggcgcaa ttcatgttgc gcatccacca
     1261 gcttgacgac ggcctcgtgg ccgaacttca tggcggcgac gatgtcctcg tcgctgattt
     1321 cctgggcggt gccctcgatc atcagcggca ggtcgcgcga ggcgacgtag accaggttca
     1381 ggtcgctctt cgccaggtcg ctgtgcgtcg gattgagcac gaactgaccg tcgatgcgcc
     1441 ccacgcgcac cgcgccaacc gggccgcgga acggcagttc ggagagcgtc agggcggcgc
     1501 tggccgcgtt gatgcagggg atgtcggact cgttctcgcc gtcggcgcag agcatggcgt
     1561 tgttgatctg gacctcgtcg aagaagccct cggggaagag cgggcggatc gggcggtcgg
     1621 tcatgcgggc cgtcaggatt tccttctcgg aggggcgcgc ctcgcgcttg aagtagccgc
     1681 cggggaagcg gccggccgcg taggtcttct cgcggaattc gacctgcaac gggaagaaat
     1741 cgatcccctc ccgcttgctc ttgccggtgg tgaccgccga gaaaacgacc gtgtcgccga
     1801 ggcggcaggt cgcggaaccc gcggcctgct gggccaggat gccggcttca aagcggatgg
     1861 ttttgccgcc gatggcgacg tcaacgtgtt tctgttcctt gatgttcatg ttctccttgt
     1921 ctttcaacgg gtccggaggg aaacgggcgt acggttcggg ccgggcctga aaagacccct
     1981 gcccgccgcc gccttcggga attccattac ggaagggact accgacgcaa gccaaggcgc
     2041 tcgacaagcg tcttgtaccg gtcctcgtgc gtgcgcatga ggtaatccag caagcgccgc
     2101 cgcttgctca ccatcacgat caggccccgg cgcgagctgt gatccttcgc atggctctga
     2161 aggtgcccgg tcagatcctt gatacgcttg gagagcaacg ccacctgcac ttccgcggac
     2221 ccggtgtcct tgtcgtgcag ctggtactct tgcttgattt ccgccttgat tgcgcttgcc
     2281 ataaccttat tccagacctg ttctggttgt tgcttcttct ctttcctttt gcttttgtcc
     2341 ggcacaccat agcggcgggt cgcggggctg tcaaagaaaa agaaaagcgc ggaagaagag
     2401 ggttcagggt gcagggggcg ccaacatctc cgcggcattc cggagcacga ggtccggggt
     2461 gatgtgccgc aggcaggcga ggtcttcgcg ctcgcaggtc cgcttgtagc acgggcggca
     2521 ggggcagtcg tccgcctgga ggacccggtg catcggcccg taggggccgg tgcgggcggg
     2581 gtcggtgggg ccgaagatcg ccaggacggg ccggcccagc gcggcggcga tgtgcatcgg
     2641 cccggaatcc acggaaatca cgaggtccat cccggccatc cagccgccca gttcgaccag
     2701 cgaggtgcgg ccgcataggt cgaggatgtt cccggacagg cctttctcga tcgccgcgca
     2761 ggtcgccctg tcctccggag agccgaaaag gaaaaccgac gccccctgcg cctggaggcc
     2821 gcgggccacg tcgtagaaca ggtccggcgg ccagttcttg gtcggccatc gcgagcacgg
     2881 caccagcgcc acgcgcgggc ccggcagcgg gatgtcggcc ggagggaacc ggaccgggaa
     2941 ggccggcggg gagaccggca ggcccaggtg ccggaccagg tccagcccct gctccacggc
     3001 atggcgggag cggtcgggcg gcccggcgcg atcggagtag agccgcggcg agccttcgcg
     3061 gccggacgcc ggcccgatgc gccgccgcga gcgagccagc cgcgcgacga tcgcgctttt
     3121 cagcaggccc tggaaatcca ggaccatgtc gtaggaggat tcgcgcaagg cccggaagaa
     3181 agcccatccg ccggccggga atctccggcg cgggaacggg atcacgcggt ccacgtccgt
     3241 gaagcaacgg accagttcgg cgtactcggt ctgggtcacc cagtccacct tggcgcccag
     3301 gccttcgcgg aggatgcgca ccgccggcag ggcatggaaa aggtccccca gcgagctcaa
     3361 cttgaccacg aggacgcggg gggagggcat gcgggggttc agcctttttc gccgacgccg
     3421 cgcttccgct gctccatcag cttgagcatg atcgccttct ggatgtgcag ccggttctcg
     3481 gcctcgtcga acaccaccga ctgcgggcca tcgatgacct cggccgtctg ctcgtagccc
     3541 cggagcgcgg gcaggcagtt catgaagatc gcgtccttgc gcgcccgctt catcagggcg
     3601 gcattgacct ggaagggcgt gaacatcttc acccgctcgt ccagcttgtg gggagggacg
     3661 tggtagctca tccacgaatc ggtatagacc acgtccgcgt cctggatcgc ctcccgcgga
     3721 tcgtgggtga ttttgaccgt gccgccggtt ttccgggcga cctgggccgc gtccttcacc
     3781 acgccggcgt cggggctgta ctccttgccc gccgggcagc cgacgcggat gtggatgccc
     3841 atcttggcgc agccgtagag cagcgaatgg gtgacgttgt tgttcccgtc gccgaggtag
     3901 gccaggatca gtcctttcag cgtctttttc ttttcctgga tggtcatcag gtcgcccagg
     3961 atctggcacg ggtgcgcgaa gttggtcagg gcattgataa cagggacggt ggcctcgcgg
     4021 gccatctcct cgatcttggc gtgctcgaac aaccgcgcca tgatcatgtc cacgtagcgg
     4081 ctcaccgtct tgacggtgtc gtagacgctt tccttgcccg cccccagcgg ggaggtgctc
     4141 gtgtcgtaga agatggcgtg gcccccgagc tgggtcatgc cggtctcgaa cgaaacccgc
     4201 gtgcgcaggc tgggtttctc gaagatcatg aggagggtct tcccctccat cgacttcgcg
     4261 aaccgcgccg ggttcttctt gatttcccgc gccagcgcga tgacgtccag gatctgcgcc
     4321 ggcgtccatt ccttcagcgt gatcaggtta tgcatgcccg cctctctgaa acaaatgcct
     4381 gtgtctgcta ctccggcgca tggcttttgg caagccgttt cctcgccggg gagggctgca
     4441 tttccctccc attcttatgc tcacactttt cgaagtgtga gcataaggaa aacagcgctt
     4501 ccggggcatc gctgcaccaa ataagtcatt attaattaca ttctaatata ttgcgatcat
     4561 gccagcgtac gaatcagcat gctcacactt ctagaagtgt gagcattatt ctggaggcgc
     4621 catcagcgcc tcgacctcgc gcagggtgcc cgccttcagc atcccccggg cctgctcttc
     4681 cgcccgccgc aggtccagac cgcgcagcac gagctgacag gccggccggc tccgcgggtt
     4741 cacgctgaac gtgcggatgc cgatgccggc caggaagggg atcatgcgcc tgtccgccgc
     4801 catgtcgccg cataccgaga ccggacgccc gcgccgcaac gccgcctgcg ccacgcggtg
     4861 cagggcccgc aggacggccg ggtgatggct ctggtacaag tcggagatct gctcattcgt
     4921 gcggtccacc gccagcaggt actgcaccag gtcgttggtg ccgatggaca ggaagtccgc
     4981 gtgggccgcc agttcctccg cgatctccat cgccgccgac acctcgatca tcgcgccgag
     5041 ccgcggcgcc gcctggtgcg gaataccctc ccgcgcgagg gcgtcgacgc acgcctgcac
     5101 gatggaacgc accgccagcc actcgtccag cgaggcgatc atcgggaata ggatcgaaag
     5161 ctcccgctcc gaaccggcgc gcaacaacgc ccggatctgc tggctgaaca cggcctggtt
     5221 ccgcagcgag aagcggatgg cccgaagccc aaggaacgga ttcgcctcgc ccggggtcgg
     5281 gtaataggac agcatcttgt cgccgccgat gtcgagcgta tggaacacga cttcctgccc
     5341 ggacagttcg tcgaggatgc gccggtagat ccggcactgc tcctcttccg acgggaaatc
     5401 atgacgcacg atgaacggga attccgcgcg gtacagcccc accccctcgg ccttcatctc
     5461 gcggacggcc aagagatcgc tcaacaggtt catgttggcc atgacgcgga accgttcgcc
     5521 cgaggcggcc gcggtttccg gggtcagatc gggcggcacc ccctccgccg cggcgggcgg
     5581 cgcgccgccc ttgatggccc ggatcatcgc ccccgaaaac gcgtcgccct tcagcatcag
     5641 caagtgagcg ctgaagatca gggcggcggc gtcctcgagc cgttgctcca tctgctgctg
     5701 aaggcgctcc agttggattt cggtctggcg cagggcccgg cgaaaatctt ccagggtccg
     5761 cgcgggcgcg ttccccgccc ccgcggtcgt cggctgatcc aggaacatga gccggccgcg
     5821 cgcaatgccc ccagcggcag ggtggccttt caggaacagc attccggatt ccggcggcgg
     5881 gacgggacgc cgcgcaggat cgggacggcg aggaacgcct cgaaccgttc ctcgtcgagg
     5941 tccggcacgg gcttgaaatg cgggtgtcgg gcggcccggc cctcgcagat cggccggaac
     6001 tcctcgacgg ccaaccccgt gatcccttcc cccagcgcca gccggacacg gcccacgctg
     6061 cccggccgca gcccctggtt cgccgccagc accaggtccc cccgcgcttc gttgtacagg
     6121 tagatggagc agaccgcggc gcgcatgtga caggccatcg cgctgacaat ggtcgcgagg
     6181 aaatcctcca gccggcggcg gcgctcgaac agcgcgccga gctccgcgac gctgcagatc
     6241 agctcgacat tgtccttttt ccagcggctc atgtcccctt ttcgacccga agtgacgcac
     6301 caattagtgt tgcgttaagc gcgatgactt gggtaatttg atcgttttat cagctcccca
     6361 caagccggga gcacgaagga tggcgccatg ccgcgaacaa cgatcaaaga tatcgccaag
     6421 gagtgcgggg tttcattgag caccgtctcg ctggtgctca acaacaatcc gcggatcagc
     6481 gccaagacac gggagaccgt cctggccagc gtcgagaagc acggctacca gccgaacgcc
     6541 tcggcccgca acctggcctc ccgcaccagc cgcacgatct gcgtggcggt gccggccttg
     6601 agccacgtct tcgccgacgt gtacttcggc gagatcgtca gcggcatcta cgaccacgcc
     6661 tccgaggccg gctacaaggt catcctcgac ctggcgaacc cgaagttcgt ggagagcaag
     6721 gaatacctga aggtgctgaa gtcgcgccgc gcggacggca tgctgttcat ctcgtcgtcc
     6781 gtccacgaca cgtacttaac ggatttcgag ggcggggcgc tcccgttcct cttggtcaac
     6841 catttcttcc ccggccgcga cctgaacttc attgcggcgg attacgagga caccgcgcgg
     6901 caggccgccg accacctcat caacctcggc cacacccgcc tcgggctcat cagcggcacg
     6961 aacacgcaca cggggctcgc gctacgcgac gcgttcctcc gccactgccg gtcgcgcggg
     7021 ctgaaggagg aggacgtggt ctggctcgac agcatgcgcg gcaaggaatg gagccaggag
     7081 ggcggcttcg acgcggccag cgagctgctc acccagcacc cggccaccac ggccatcatg
     7141 tgcggcaacg accgcctcgc catcggcgtc atgcgccacc tgcgcatgcg gcggctgcgc
     7201 atccccgagg acatctccgt cgcgggcgtg gacgacatcc ccgaatccac ctacaccaac
     7261 cccggcctga ccaccgtccg gcacgacctc tacaagatgg ggcgcacggc ctgcgagcgg
     7321 ctgctcgcgc tcttccgcgc cgagatcgcc gagtgccgcg agatcctgcc cgtccagttg
     7381 atcgcccgcg aatccaccgg cccggcccgc ccggcttgag gactttttca cctcagaggg
     7441 caaagaggta cacagagggg catgccgaaa cggtgacgac caccttcttc ctccacgttc
     7501 tccgtgacct ctgtggttaa aacgaaaacc tgctttcatg cgcgtcacat ggctgatcgt
     7561 tgacgggtac agcctgctgc accgcttcga cccggcgggg gcgcggagaa agggcgggct
     7621 ccaggccgcc cgccagaaac tggtccgcca cgtcgaggac ctcgccgcgg gccgctacga
     7681 ccgcgtgacc atcgtgttcg acggcaagga atccggcggg ggcgaaggct acgaatcccc
     7741 gcacgtcgaa atcctgtttt ccccggtcgg ccgcaccgcg gataccgtga tcgagcggct
     7801 cgtccacgac gccccggagc ccgaggccat cctcgtcgtc agctccgacc ggctcgagcg
     7861 ccagaccgtt tccggccgcg gtgcgcagac cgtcggctgc ggcgacttcc tcgccgaggg
     7921 cgagcagcgc cagcggcacc gcccggccct atcttcgcgc cacccccgcc cgacgctggg
     7981 ggatttcttt ccggaccgcg catgagccca gcttccggtt gactccgccg gactcttttg
     8041 gtcgaatatc cgccgaggaa cacctgcttc caaggagtcg ccatgaacgg gttacgccgc
     8101 ggtctctgcc tggggttcgg cctgattctc atcgccggcg ccgcgcgcgc ggcgaccctg
     8161 ctcgtccgga ccaacagccc ctcccccgcg ccgccgtacg ccgactggga ttcggccgcg
     8221 cacacgatcc aggacgcgat caacgaggcc ggggcgaacg acaccgtgct ggtcaccaac
     8281 ggcgtctatg acgcgggcta cacatacatg ccgggctata ccctggcctg ccgggtggtg
     8341 atcacgaacg gggtgcaggt catcagcgtc aacggcccgg cggagaccgt catcaagggc
     8401 gaaggcgcgc tggactcggg cgcgatgcgg tgcgccttcc tctccgaggg cgcgacgctg
     8461 tcgggcttca ccctgaccaa cggcttcacg cgcacggcgg gctattacta tttcgacatg
     8521 agcggggccg gcgcgcacat cctgaacagc gggacgatca ccaactgcgt catcaccggc
     8581 aacacggcct cgtaccacgc cggcggcgtg atgtgccaca acggcgggct cgtcgtggat
     8641 tccatcgtgt gcggcaacct cgcccacgac aacgcgggcg gcggggtcta cctgtaccat
     8701 aacggcacgg tgagcaactg ccaggtgtac gacaacaccg cgtggtggaa cggcggcggc
     8761 gtcgaggtca gctataccgg cctggtgaca cacagctccc tcttcgcgaa ccgggcgcaa
     8821 aacggggacg gcggcggcgt ggacctgaac tacggcggca cggtccggga ctgcctgatc
     8881 tacagcaacc gctccaccgg cgcgacggag tacgacggcg gcggcggggc gcagtgcttc
     8941 ttcgggggca cgatcaccaa ctcggccgtc tacggcaaca gcgccgtcac gcgcggcggc
     9001 ggcgtgaaga tcgtgttcgg cggcgagctc gtgaactgct cgatcttcga caacaccgcc
     9061 ggcgacggcg gcggcttcta cggcgattac tacacctcca acaagaattg cgtcatttac
     9121 cacaaccgcg ccctcgtcgg ccccaactgg agcaccaacg gcagcggcaa ctacttctgg
     9181 gcgaattgtt taacgccgct ccccgccgga ccgggcggcg gcaacctcac gaacgccccg
     9241 ctgctggcgg gcatccggaa ctggcacctg ctgaccaact ctccctgcgt ggacgcgggc
     9301 tcgaatgtct tcgcgacggg catcgtgctc gacctggaca gggaagcgcg gatcgccggc
     9361 gcggcggtgg acatcggctg cgacgaacgg accccggccg ggctggccgg cccgctcacg
     9421 gcgctgatcc gggcggcggc cacgcaggtg gtcgtgaata tcccgctgac cttctactcg
     9481 gacgtgtccg ggaaggtcag ccggatcgag tggacgatcc agaccgaatc cggcccgcgc
     9541 gccaccacga acctgttctc cgaagcgcag gcctggtccg tgaccgggcg gttccccgtg
     9601 gtcctggccg cgttcaacaa cgactcgcac gccgccgtca cggccgtggt cgaggtgctc
     9661 gccggcttca ccaactacgt gtcggagttg agcccttcgc cggtcccgcc ctataccagc
     9721 gaggcgacgg cggccacgag cctgtatgaa gccgtccagg tcctgccgcc cggcggcgtg
     9781 acgctgatgt actacggcaa ctacccggag gcgcacgaga tcatcttcga caagcccgcg
     9841 acggtcatcg gcacgttcgg gccggcggcg acgcggatca gcgggctggg cgcgcaccgg
     9901 gtcatccgca tcagcagcgt ggacgcgcag ttgcacggcc tgatgatcac ggacggcgcg
     9961 gcgggcgcgg agccgggcgg cggcgtgctg atggagacgg ccggcgtgtt gagcaactgc
    10021 tggatcatcg gcaacgtgtc gagcaacgac ggggcgggcg tgtacctgaa cgccggcggc
    10081 gcgatgttcg actgcctgat cgccagcaac cggggcgtgg cctggggctg cgacggcggc
    10141 ggggcctact tcaaccgcgg cgggacgtcc cgcggctgcc ggtacgaggg caacttcgcg
    10201 gtcgggtacg gcggcggggc ttacttcaac cgcggcggca cggcggacga cgcggccttc
    10261 ctgtcgaacc tgtccagcaa cgggggcggc ggcgcgtact gcaactacgg cggctcgatg
    10321 tgcaacgcgg ccttcctggg caacgcctcg tactccggcg gcgggctgta cctctactac
    10381 ggcgggcgcg cgatgaacgc gcacgtggcg agcaacacgg ccgagcattc cggcggcggg
    10441 ctgtacgtgt acggcaacgc ctcgcccacc tgctcgcacg cgacggtcca ggggaacatc
    10501 gccggggtcc ggggcggcgg cgtctacgcc aactccggcg ggaatatcct gaactcgatc
    10561 gtctattaca accgcgcgcc gatcggcgag aacttcttca acgactggtc cggctaccgc
    10621 tacaacacgt gctgcatgac ccccgcgccg gcctccggcg ggtacaacat cctgaccaac
    10681 gcgccgctct tcgccggcgc gctgaacccg cacctgctgt ccaactcgcc gtgcctcaac
    10741 atcggctcca acgcgttcgc cgacggcatc gccgcggacg tggacggcga gccgcgcacg
    10801 aacggcgtcg tggatctcgg ctgcgacgag ttctgggccg agggcatgac cggaacgctg
    10861 tcggtcgcga tcctggcccc ctacaccaac gccgtcgcgg gcacccccct gcccttcgag
    10921 gcccatatcg aggggaaggc caattcctac gagtggcggt tcggcgacgc cggggcgacg
    10981 aacggcttca cgaacctcct cgcgctcacg tacacctggc cgtcggcggg cgcctacccc
    11041 gtcgtcctgg aggccttcaa cgagagcggc agcgcggcgg cgacggtcac ggtgacgctg
    11101 gtggaaggtt tcacgaacta cgtggacctg gccaacccga ccccggcgtc gcccttcacg
    11161 aactgggcga cggcggccaa cacaatccag gaggcggtgg acgcggccgc ggcgggcggg
    11221 ctggtgctga tcaccaacgg cgactacaac ccggcggcga cgatcgccct ctccaagccg
    11281 gtgcggcttc agtcggtcaa cgggccggac gccgtcacgg tcagcggcca gggcgcgcgc
    11341 cgcgtgttca acgccagcgc gcccggcgtc tcgctcgacg ggttgacgat caccaacggc
    11401 ttcacggcgg acaacggcgg cgggatcctc tgcggggccg gcgtgtcgat caccaactgc
    11461 cggatcgtca actgccaggc ggccaacggc gcgggcgtgt acttcaacgg cggcggcgcg
    11521 ctgttcaact cgcacctgca gtacaaccgg gcggacacgg cgggcggcgg cgcgtactgc
    11581 gccatcgggg ggctggcgca ggcctgctcg ttcttcagca acgtcacggt gtccacgtcc
    11641 ggcggcggcg ggggcggcgc gtacgtgaac accctcggca cgttcctgga ctgcgtcttc
    11701 acgaacaacc tctcccacta ccgcggcggg gcgggctacg gctactacgg cggcctcttc
    11761 agcaactgcc tggtcgcgcg caaccaggcg aagggctggt cgtcgtccgt cgcgggcggc
    11821 gggctctact tgtcctacgg cgggctggcc caggcgtgca cgatcagcaa caacgtctcg
    11881 gacgagaccg gcggcggcgc ctacctctat tacggcggcg ccgtggagga ctgcctggtc
    11941 tccaccaact gggccgagga cggcggcgga ggcgtctaca tcaattcgat cggcacagtc
    12001 cgccgctctc gcatcgagaa gaacgccgcg cgctgggacc agggcggagg cgtctacttc
    12061 taccgcggcg ggatggccga ggcctgcgtc ttcgcggaaa accgctcgtt gagcacgtgg
    12121 cagggcggcg ggggcgcgta cgcggaccgg gcggcgctgg tgctgaccaa ctgccagttc
    12181 tggggcaacg aggcgtacag cggcggcggc ttgtacatca gcgaatatgg ctcggccgtc
    12241 ctgtgctcct tctggagcaa ccgggcgacg cagggcggcg gcgcctacct gaacaacggc
    12301 ggccggctcc accggtgcgg cttcaccaac aacgtcgcga accagggcgg cggactgtac
    12361 gtcttcgacg cgggcgcggc cagcaactcg atgtttatcg gcaacacggc catggacggc
    12421 ggcggcgcgc acctggtcaa cggcggcgac ctctacaaca gcttcttcgc cgggaacgtc
    12481 gcctcgaacc agggcggcgg cgtggccctc gcgtcgtggg gcgagtacag cacggtcgcc
    12541 cactgcacga tcgtcggcaa cacctgctac ctccagggcg gcgggctctg gagcgacctg
    12601 ggcgggaccg tgcggaacac gatcctgcgc gacaaccttg cggcgttcgg ggacaactgg
    12661 ttccacgaga ccggcgccgc gccgacgtac gagcattgct gcgtcgagcc cgaccccggc
    12721 gggaccggca acctcacgaa tgcccccctg ctggcgggcc ggctcaatcc ccacctgctg
    12781 acgaattcgc cctgcgtcga cgcgggggac aatgcctggg ccatcggcac ggacatcgac
    12841 ctggagcccc gcatcgccgg gccggccgcg gacatcgggt gcgacgagat ggacccgacg
    12901 aacctcgtga gcgaaatccg gggcggcatc tacgccccgg gcaccaacgt gaccgtccag
    12961 acgcccctca cgttctactc ggacgtccag ggccaggtgc ggtggatggt gtggaacgtg
    13021 aacgacccgg acgcgccgga cggcctggcc cagttcacca acgtgccgtc cgtcacttac
    13081 gcctggaaca cgcccggcga ctaccagatc atgctgcagg cgttcaacgg gatctggtac
    13141 ggggacgggt tcgtcaccgt gcacgtgacg gaggcgcaga ccaactacgt cgcgccgggc
    13201 ggggcgcacg tctggccgtt cgccagctgg gccaacgcgt ccacgtccat caccgaggcc
    13261 gtctccgcgg cggcctacgg cggcgtggtg gtcgtgtcga acggcgtgta ccgcgagccg
    13321 gcgggcatcc tggtggacaa gcccctggtg atccgcagcg tcaacggctg ggaccacacg
    13381 acgctggacg gcgagggcgc gcaccgcggg ttcgtcctgt tcgagaccaa tgtcctcgtc
    13441 gaaggcttca cgttcagcaa cggcctggcc cacgcggaca tgttcggggg cggcggcggt
    13501 gtggtcctga tcagcggcgg cacggtgcgg cactgccggt tcctgaactg cagcgcggac
    13561 cagtacggcg gcggcgtgct gtgctactac ggcggctcgg tgcagaactg ctggttcacg
    13621 ggcaacacgg cgatgttcgg cggcggcgcg ctctgctact acggcggcga ggtgatcaac
    13681 tccgtcctcg tccgcaacca ggcgctggcg cagggcggcg gcgtgtactg cgagggcggc
    13741 gggggcgtgt acaactccat cgtgtaccac aacctcggcc ccatggggcc caactggctg
    13801 aacgtcgccg gcgggatcac ctatgaaaac gtgtgcaccc agcccctgcc ccccggcccg
    13861 ggccacctga ccaacgcccc ggggctcgcc gggctcaaca acccgcacct gctgaccaac
    13921 tccccgtgct tcaacgccgg ctcgaacgtc cacgcggcgg cgatcgccgc cgattgcgac
    13981 ggcgagacgc gcgtcctcga cggccgcgtg gaaatcgggg ccgacgagat ctacccgccg
    14041 ggcctcaccg gcgccctggc cgtcgccatc gcgggggacg cgttcgccac cggaccgggc
    14101 ttcccgctgc aattctgggc gctcgtggac ggcaagcccg cgcacaccct gtggaccatc
    14161 gacgccggcg gcttcggcac ggccgtcgta tccgacgctc tgaccgtttc ttactcctgg
    14221 tccgtcaccg gcagctaccc ggtggtcctg tccgcgtaca acaacaccga ttacgccgcc
    14281 gcgaccgtga cggtcaccgt ggccgacggc acgatccgct acgtggacaa ggccggcgcg
    14341 cacaacccgc ccttcctgac ctgggcggac gccgcgcatt cgatctcggc cgccgtcgcg
    14401 gtcgcggtgg ccggcgacac gatcctgatc accaacggcg tgtatgccga ggggacggag
    14461 ctgatcgtga acaagccctt gcggctggtc ggcgtcaacg ggccggaggt cactgtcgtg
    14521 gacggcgcca acagccaccg ggtgttccgg ctcgaggacc cgctggctgt cctggagggg
    14581 ctgggcatca cccagggcag cgcggcgggc agccccggcg gcgagggcgg cggcgtggtg
    14641 ctcaacggcc acgggcaggt ggtcaactgc cacatctaca gcaaccagtc gcccggccac
    14701 gccggcggcg tgctgctgta cggcggcggc tcggtcaccg ggtgcgtgat ccgccacaac
    14761 acggccgagg agggcgcagg cgcaacgctg ttcaacggcg gcctgcttgc gggctcgcgg
    14821 ctgtgggcca accaggccac gacgcggggc ggcggggcgc tgctcatgat gggcggcatg
    14881 gtatccaact gcctcatcca gcagaacaac gccaacatgg agggcggcgg cgtggcgttc
    14941 tacgagggcg gcacctgcgc ggccagcctg atctcgtcca acctctcctc cgagggcggc
    15001 ggcgcctact tctacctgga cggcacgctg acccactgcg tgctgcagac caacttcgcc
    15061 tggaacatgg gcggcggcgt ccggacctac cagggcgggc tgatccgcaa ctgcctgttc
    15121 taccacaacg ccgccgaccc gcagtggggc ttcggtagcg gcggcggcgt gttcctgcgc
    15181 ctgggcgggc tggtggagaa ctgcacggtc gtggagaact acgcgtcggt cggccggggc
    15241 ggcgggattc tctgcaacag cggcggcacg gtgctcaaca gcatcgtcta ttccaaccac
    15301 gcggaaaaca tcgccaaccc cgagcaaagc gcgttcagct actgctgcgc ggagcccgcg
    15361 ccggccgggc tcgtgaactt caccaacaac ccgcgcttcg agagctggac ggcctgggac
    15421 taccgcctgg cctcgaactc gccctgcgtc gagcaggggc tgaaccagga ctggatgacc
    15481 aacgccgtgg acctcgacgc caccgcgcgc ctcaagcacc tctacgccga catcggcgcg
    15541 tacgagagcg tctggaccaa cgtggacacc gacggcgaag ggctcgtcgt cggcatcgag
    15601 cgcagccggt tcggcacgtc cgacttcctg accgacaccg acggcgacgg gcaggacgac
    15661 tgggaagaag ggatgatcac cggcacctcg cccaccaacg gcgcgctcta cttccggggc
    15721 ctgatcgggc cggacgccgc cgggcggtcc gtggtccgct ggcagggcat cacgaaccgc
    15781 gtgt
//
LOCUS       DAOOTK010000071        15781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2531_length_15781_cov_4.985820, whole genome shotgun sequence.
ACCESSION   DAOOTK010000071 DAOOTK010000000
VERSION     DAOOTK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15781
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_15781_cov_4.985820"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..811
                     /locus_tag="PK634_08890"
     CDS             131..811
                     /locus_tag="PK634_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOW97890.1"
                     /translation="MCSSPRSPNSRPDPADRACTVIGLAVLLAGILPLLAWEWTGPRS
                     GEVELFPETALRLALAPVPAVAAPPPEPPALEPPPPASEPEPAAPPESAPAPGPAPES
                     APPEIPPAPSEAEQPPVETPGDAGREEAIQEQWFGELRRRIEENKFYPGGARFSKETG
                     TVTLRVRISAGAEIIGSEVVSNTGSGRLAEGAQAILRRAAASPLGTNRLAAEIQAQVP
                     ITYRMTGR"
     gene            complement(828..1178)
                     /locus_tag="PK634_08895"
     CDS             complement(828..1178)
                     /locus_tag="PK634_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW97891.1"
                     /translation="MANPELKMFTEQIGNVTVVTVAGPVDSATIEQFRMALDPVAAKP
                     GGYVLLDCQELTYINSRGLGLLAKYHRTCFGHLGWFALCNVNRKLVRTMDLLGLGSLL
                     KFYDTRDAALAAKL"
     gene            complement(1282..2103)
                     /gene="budA"
                     /locus_tag="PK634_08900"
     CDS             complement(1282..2103)
                     /gene="budA"
                     /locus_tag="PK634_08900"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOW97892.1"
                     /translation="MIRIFGIGLAAGLALLAPGCAHRAARGPDLVTQYSVIDALLAGD
                     YDGRLPCSELRRHGDFGIGTFDKLDGEMTLLGGAVYAIRADGRVEKAPGAETTPFATV
                     AFFHPDQYEGRPLSGLTYAAFQEDLSRQLPTPNHFYAVWAYGRFHRVTTRSVPRQAPP
                     YVPLAEVTKNQSVFEFTDVEGNLVGFWSPPFAKGVNVPGWHFHFLTRERTGGGHVLDF
                     ELRDGAAWLDGKSAVTVLLPDAGDFLRTDLAPDRAEELRRVETDPLKAGASAGDR"
     gene            complement(2113..3066)
                     /locus_tag="PK634_08905"
     CDS             complement(2113..3066)
                     /locus_tag="PK634_08905"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOW97893.1"
                     /translation="MIRGSAWVSMCSALLVLAGAQVSLALHASQDPLLEPSKEKPYAF
                     SLRGSVGLLDGQADELVYEPEMDDYKLSELNWDLSGLAMGGAVLSAAAGKLGCNAGFW
                     VALTQGDGEMKDYDWFIPGMDWTDYSRSEVDVESAYSLDINANYLIYGRPEIGIRGVL
                     GFKHDYWEWSDSGQEFIYSTFGFRDTIGSFEGQNVIDYQQTFDIPYVGLNLDGRAGPL
                     GWNAYALYSPLVSAEDKDHHILRDLHFEEDFDGGDFFALGVSGSYRIGKQFFLSGAVD
                     YQDIPEITGDMLIVEDGESYEDSAGIANNNLMVSLAFGWLL"
     gene            3207..4850
                     /locus_tag="PK634_08910"
     CDS             3207..4850
                     /locus_tag="PK634_08910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW97894.1"
                     /translation="MDPAPTAAGRLFARGGAPAIFLVALLARLAVLLPLHAHPLYGVP
                     IVDAASYADQARALAAGAPAGEALFWQPIFYPLWLSVVFRAFGPSLLAARMLQAVLGA
                     AACVLVYFLTRRLGGRLTAVAAGLILALYGPVIALEADLLSEGWAVFWTALLLWLLQR
                     AQTEPRPGTLAALGVAGGLAALTRPTFLPFTLGAGLWLTALRVRRYGWKAGLDTAGTL
                     AMGLLAVLLPAAAFGRQHTGRFTAFPSSSGLNLYLGNNPRWIETVSIRPGVEWMELIR
                     EPQRAGLSDIWDQSDYFRNKVAGYARSQPGAFAAGLLQKAARFLCSREIPRNHDLYSL
                     REAAPVLKPLLWKAGGFGFPFGLLLPLALLGLAARRRQLGAAWLFLLLYSPAVIAVFV
                     SDRYRAPAVPALAVAAALGLAWLWEQRANRTRLAGALAALAAAAALTSVARAYPEEQL
                     AFEAERNWAAGQRLAAEGRPAEAEACLRRATEIDPRMVDAWNSLGVLLEQGGRRADAI
                     DAYRRAVALDPSNEGPRFNLEAARLPASGSSAPAAGPSP"
     gene            complement(4783..6042)
                     /locus_tag="PK634_08915"
     CDS             complement(4783..6042)
                     /locus_tag="PK634_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOW97895.1"
                     /translation="MSADVRIRAPERLRVAGSSLWLVLREAGQSFSANRDMASAATLA
                     YYGFLALMPLLLLVIYLLGLFLQSSEAVLAGVEDLAARLFPSFNRALLVDLLSVARGR
                     AWGLISVVLLFWSLMPFAGAIRAAVRRIFRAAHRASFLRSKLLDLSAVLVLLLLFLLM
                     AGGKVYASLGPVRLLARAGAALACARALGSAALAVGVLAFFYRVFAPGKIRLPHLLAG
                     ALAAAALLAVVRPLFGLILKYNPNYGYAFGSLKAIFLLLVWAYYTFAVLLFGAEVVAM
                     TQRRDALLLRRLFAGGPEARTSRVLLGRFVRAAADGEVLFREGDPGAEMFYVLEGSVR
                     LTRAGRELRVMGPGEYFGEMSMLLGAPRAATAVASGPGTRLAAISQKNFEVILGENPA
                     IVFDLLKEMARRLEQTNRKLEAGPPRD"
     gene            complement(6032..6601)
                     /locus_tag="PK634_08920"
     CDS             complement(6032..6601)
                     /locus_tag="PK634_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOW97896.1"
                     /translation="MAMSFLELAARRESTRRYAPAPVPREALERCLEAARLAPSACNS
                     QPWKFIVVDSEPLRGRLVGEAFKGIYRMNRFVEQAPVLIVIVTERSKYAAQLGGQFRG
                     IQYSLVDIGIAGEHLALQAAEEGLGTCWLGWFNERGVKKVLRLPRSARVDIILSLGYP
                     AEPGPREKKRKTLDVIREYRVDPVKAHER"
     gene            complement(6626..7303)
                     /locus_tag="PK634_08925"
     CDS             complement(6626..7303)
                     /locus_tag="PK634_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOW97897.1"
                     /translation="MTWLVSSTLRDEADRRYARWLASAGVPAEWVRPGDPVPASFEAL
                     LLTGGGDVDPDLYGAARQPRTDAVNRARDVFEIMLVRAALEARRPVFGICRGIQVVGV
                     ALGGRLIQHIPDICPAAREVHAQVEGRDSGHPVEVEARSRLASVLGPRTDVNSAHHQA
                     VDPAAPGRGLKIAARSPAGIVEAVEADGIPVMAVQWHPERMPPDHPASRALLQLFRTI
                     ASERDPA"
     gene            7353..7988
                     /locus_tag="PK634_08930"
     CDS             7353..7988
                     /locus_tag="PK634_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOW97898.1"
                     /translation="MNIECIVVGPFEVNSYIAHRGAGGEALVIDPGADADLLRDALRR
                     LRLRPAAYLLTHGHVDHVSALAALHREWPAPVILHELDAPWAFTPVNELPPYYAAPAA
                     PAGAPCLVKDGEARTDGPFRYRVIATPGHTPGGVAFHLPDERVLFAGDTLFQGTVGRT
                     DLPGGDARALARSVLQLGELPDDTRVFPGHGPATTIGEEKRSNFFMHRAAS"
     gene            complement(8004..8903)
                     /locus_tag="PK634_08935"
     CDS             complement(8004..8903)
                     /locus_tag="PK634_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97899.1"
                     /translation="MGRGKDPAYRITEERRPGSSGPVAHILGVLVCLAGIVYLGLFLA
                     SRTQGFRLSVEDRLERALGLPVSLKRVEATPGLNLRLEGLASRDSEKNGRPGIRAHEV
                     VLGWSLSGLVRPGPLFQRVVIRGGAITFAPGSSGRWEPAALEPLGAQVAQWGGFELPA
                     PEAAGSSAPGDGNEAGPAAPSAEGAPTNTASWFEHVALEVRAARLAWWDAEGGERASA
                     EGIDLLVTPVNLPNRPITHVHLTMDKGRVGNRQVQNLVFELIRTAEQDIILGYAGDWH
                     PVAKGEPADAPEKPAGENPVTPD"
     gene            complement(8914..9741)
                     /gene="proC"
                     /locus_tag="PK634_08940"
     CDS             complement(8914..9741)
                     /gene="proC"
                     /locus_tag="PK634_08940"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOW97900.1"
                     /translation="MSVAKPDVGNVVFIGAGNMAEALVKGILGGGLCPRNAIGVTDVR
                     PERREYFEKHFGVRALTANREAVRRADVVVLAVKPQAMAEVLGELRGAIPKTALVMTI
                     AAGIPTTSLEDGLGEGTRVVRVMPNTPSLVQAGASALCGGRWATEPDLATAGALMASV
                     GLVARVKEADMDAVTAVSGSGPAYVFFLAEVLLHAAEKMQLDAGTARDLTVATIEGAA
                     RLMKETGLPPAELRARVTSKGGTTEAALNVLREKGVFESFVEAVLAARRRAAELLKS"
     gene            complement(9738..10430)
                     /locus_tag="PK634_08945"
     CDS             complement(9738..10430)
                     /locus_tag="PK634_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOW97901.1"
                     /translation="MDSGLAERLQAVRERMESACARAGRDPGGARLVAVSKTHGPDRV
                     REAVECGLTVFGESKVQEAQAKIPQCPGRLSWHLVGHLQRNKAAVAAQLFDYIHSVDS
                     ARLLEAVDRASAEAGKVMPVLLEVNVSGESSKFGLAPADVPALLARANELDNVEVRGL
                     MTMPPFTEDPAEARPWFRRLRELRDAWRGETGFALDELSMGMTHDLEIAIEEGATWVR
                     VGTALFGERERT"
     gene            10541..10849
                     /locus_tag="PK634_08950"
     CDS             10541..10849
                     /locus_tag="PK634_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HOW97902.1"
                     /translation="MSRQETISLEQGRAEGHVSPIGPVNLVWITAPNGMIGCGAFDVV
                     GLERFRYAAARMKGAGGKVIASIEDLLEGEVREANAFAREKGIEAGLSGRAALAKLLG
                     "
     gene            complement(10851..11204)
                     /locus_tag="PK634_08955"
     CDS             complement(10851..11204)
                     /locus_tag="PK634_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97903.1"
                     /translation="MPDHFFRSVFQNPDYAVARTDAGAVRDRIEIQTLRAEVERLLMI
                     AEALWSILAEKFDLPEEELLRRIHEIDRRDGKLDGRVAPEPPSLCPKCSRPMEKRRPY
                     CMYCGQPVARDPFER"
     gene            complement(11241..12839)
                     /locus_tag="PK634_08960"
     CDS             complement(11241..12839)
                     /locus_tag="PK634_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOW97904.1"
                     /translation="MRGIRFHRLAGLLLSAGTALAAESPFVVTASFDQREGQPHVRVH
                     FQYPPGHYLYADSIHVAARAGAELVLAEAPTPTRVRDKHSGGEKAVLDKPHDRLYQLP
                     VAAGVLEVEVSFQGCDASVCYFPEKRIVPVGAAAGDSPALPVAPGPASPAAGDWRAAL
                     ERFSVAGTAAGYVKPEALLEFLGQAESGAAAGDGLVARWQKLGWLATLALILAGGVAL
                     NLTPCVLPMIPVNLAIIGAGARAGSRRRGFALGGVYGLGMALAYGLLGVIVVLTGARF
                     GALNASPWFNAAMAGLFVALSLAMFDVFEINLSRFQGGGAEGRRGGYTVALVMGAVSA
                     LLAGACVAPVLISVLVLAGHLYGRGIALGLLLPFVLGLGMALPWPLAGAGLSFLPKPG
                     RWMTVVRNLFGVLILLLALYYGYTAYRLFRPPPPPPAVKTGASNLRKVDSAEGLAAAL
                     DEAAREGRPVFLDFWATWCKNCLVMEKTTFKDPAVAARLAGYLVVKFQAEQPGDPPAS
                     DILDRFGAIGLPTYAVLKPVAGSR"
     gene            complement(12851..13408)
                     /locus_tag="PK634_08965"
     CDS             complement(12851..13408)
                     /locus_tag="PK634_08965"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOW97905.1"
                     /translation="MNFRPRIKGWAAFVAVALAGCGPSGEDRAAQPEVSPSGPAASAF
                     FIPSLKAAGESIDLNSFRGRVALLDFWATWCPPCRSELPELARLFEELKDQGFVLVGM
                     TVDQGAAESVAQAVGRFEIPYPVGLAGPDIQAAYGGIRAVPTKFLLDAQGKVRKSYLG
                     VVPVEQLRADISALLAETKGAPASP"
     gene            13678..14562
                     /locus_tag="PK634_08970"
     CDS             13678..14562
                     /locus_tag="PK634_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97906.1"
                     /translation="MKKRTRWGPPGWPWELELLALVFVVTAIVVPYYLQAQTTESPQL
                     SILAQAEQAQASQRGSWQDLQEFIWAVIIVGLYLAHLVVASSSIDYMSTPFTHLFSPL
                     LFSMITYYRLYTLVKDSPTATAIVSGSPLEIALWVLGVLVITFMVARIRMARYMLNFK
                     HVDWDLTTPTLFDRSFWELAVYLRPLIYPPRLYRACPNGILIEGWVYAMPIPFETIHS
                     VDAVQGAGFLSSGYCLATSAKSLVRLQISEKAEPLLISPKDRAEFVRYCEQRLSTTRL
                     IKIPSETRHGAAGTTTRG"
     gene            14579..>15781
                     /locus_tag="PK634_08975"
     CDS             14579..>15781
                     /locus_tag="PK634_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOW97907.1"
                     /translation="MNPIGDSRPQEAAREMVRTLRAAGFDAYWAGGCVRDRLMGRPAK
                     DIDIATSARPEQVQALFPKTLEIGKAFGVIQVLRDGFAFEVATFRHDRPYRDGRHPDG
                     VAFTSAEEDAKRRDFTVNGLFFDPIEDRVLDFVGGQDDLQRRLIRAIGDPEARFREDH
                     LRLLRAARFASALEFDIEPATAEAVRRLAPLLADVSAERVQQELTRLLVESPRAGRGL
                     ALLRDLGLLAVVLPEAERLVGQEQPPAFHPEGDVFTHTVLMLDAMKDATPELAWSVLL
                     HDIGKPLTATPTIEPDGSTRIRFNEHDSVGADLAEQILRRLKMPNDLVDAVRQGVANH
                     MRFINVPRMRRSKLRQMAGAPTFPMELELHRLDCLASHSDLSNYEFMRRFMEELKNEP
                     VLPPPWIRG"
BASE COUNT         2466 a         5696 c         5333 g         2286 t
ORIGIN      
        1 aacaaggcgg acctgccggc ccggctggag gcggcaacgg aacagccggt ggaactgcac
       61 gtggaccgcg ccgcgccgtt ccagtcgttt atctccgtgc tcgaccggat gcgcgcctgc
      121 gggagaaacg atgtgctcat caccgcgcag cccgaatagc cggccggatc ccgccgatcg
      181 cgcctgcacg gtaatcgggc tggcggtcct gctcgcggga atcctcccgc tcctggcatg
      241 ggaatggaca gggccgcggt cgggcgaggt cgagctgttt ccggaaaccg cgctgcgcct
      301 cgccctggcg ccggtccccg ccgtggccgc cccgcccccg gagccgccgg ccctcgaacc
      361 gccgcctccc gcctcggagc ccgaaccggc cgccccgccg gaatcggcgc cggcacccgg
      421 gccggccccc gagagcgcgc cgccggaaat tccgcccgcg ccgtccgagg ccgagcagcc
      481 gccggtggaa accccgggcg acgccggccg ggaggaggcg atccaagagc aatggttcgg
      541 cgagctgcgc cggcggatcg aggaaaacaa gttctatccc ggaggcgccc gtttctccaa
      601 ggagaccggg accgtgaccc tgcgcgtgcg catcagcgcc ggcgcggaga tcatcgggtc
      661 ggaggtcgtg tcgaacacgg gctcggggcg gctggcggag ggcgcgcagg ccatcctgcg
      721 gcgggcggcg gcgagcccgc tcggaaccaa ccggctggcc gcggaaatcc aggcgcaggt
      781 gccgatcacc tatcgcatga ccgggcgttg agcgatggcg gggcggctta cagcttggcc
      841 gcgagggccg cgtcgcgcgt gtcgtagaac ttcagcaggg accccaggcc cagcaggtcc
      901 atggtgcgga caagcttccg gttgacgttg cagagggcga accagccgag gtggccgaag
      961 caggtccggt gatacttggc cagcaggccg aggccgcggc tgttgatgta ggtcaattcc
     1021 tggcaatcca gcaggacata tccgccgggc ttcgccgcga cgggatcgag ggccatgcgg
     1081 aactgctcga tggtggcgga atccaccggc cccgccacgg tgaccaccgt gacgttgccg
     1141 atctgttccg tgaacatctt gagttcggga ttcgccatcg ctctcctttc accctccacc
     1201 cggcaccgtc gcgaatctat ttccttttac tcaagaacga cgcccgcgtc aagaaagacc
     1261 gccatgaaaa aacgagctcg gctatcgatc ccccgccgaa gcgccggctt tcaggggatc
     1321 cgtttcgacg cggcgcagtt cctccgcgcg gtccggggcc agatcggtcc gcaaaaaatc
     1381 gccggcatcc ggcagcagca cggtgaccgc cgatttgccg tcgagccacg cggccccatc
     1441 gcgcaattcg aaatccagga cgtgcccgcc cccggtccgc tcgcgggtca ggaagtggaa
     1501 atgccaaccc ggcacgttca cgcccttggc gaacggcggg ctccagaagc cgaccaggtt
     1561 cccctccacg tcggtgaatt cgaaaacgga ctggttcttc gtgacctccg ccagcggaac
     1621 gtagggcggc gcctgccggg gcacggaacg ggtcgtgacg cggtggaagc ggccgtaggc
     1681 ccacacggca tagaagtggt tcggcgtggg caactgccgg ctcaagtcct cctggaaggc
     1741 cgcgtacgtg aggccggaca gcggccggcc ttcatattga tccggatgga agaaggctac
     1801 cgtcgcaaac ggggtggtct cggcgccggg cgccttttcc acgcgcccgt cggcccggat
     1861 ggcatacacc gcgccgccca gcagggtcat ctcgccgtcc agcttgtcga aggtcccgat
     1921 gccgaaatcg ccgtgccgcc gcagctccga gcacggcagg cggccgtcat agtcgccggc
     1981 caggagggca tcgatcaccg aatactgggt gacgagatcg ggcccccggg cggcgcggtg
     2041 cgcgcaaccc ggggccagca gcgccagccc ggcggccagc cctatgccga agatcctgat
     2101 catgagaccc gtttacagga gccagccgaa ggccagcgag accatcaggt tgttgttcgc
     2161 gatgccggcg ctgtcctcgt acgattcgcc gtcctccacg atcagcatgt cgcccgtgat
     2221 ctcgggaatg tcctggtagt ccacggcgcc ggacaggaag aactgcttgc cgatccggta
     2281 actcccggac acgccgagcg cgaagaaatc gccgccgtcg aagtcctcct cgaaatgcag
     2341 atcccgcagg atgtgatggt ctttgtcctc ggcgctgacc agcgggctgt agagggcgta
     2401 ggcgttccag cccagcgggc cggcgcgccc gtccaggttc aggccgacgt aggggatgtc
     2461 gaaggtctgc tggtaatcga tgacgttctg cccctcgaag ctcccgatgg tgtcgcggaa
     2521 gccgaaggtg ctgtagatga attcctgccc gctgtcgctc cactcccagt agtcgtgctt
     2581 gaagcccagc acgccgcgga tcccgatctc cggccggccg tagatcaggt aattggcgtt
     2641 gatatccagg ctgtaggcgc tttcgacgtc cacctcgctg cgggagtagt cggtccagtc
     2701 catgcccgga atgaaccagt cgtagtcctt catctccccg tcgccctggg tgagggccac
     2761 ccagaatccc gcgttgcacc cgagcttgcc ggcggccgcg gagagcacgg cgccgcccat
     2821 ggccagcccg gacagatccc agttcaattc gctcaacttg tagtcgtcca tctccggctc
     2881 gtagaccagc tcgtcggcct ggccgtccag caggcccacc gagccgcgca gcgaaaaggc
     2941 gtatggcttt tccttggacg gttccagcaa cgggtcctgc gaagcgtgca gcgccagcga
     3001 cacctgcgcc cccgccagca ccagcaaggc cgagcacatc gacacccagg ccgatccgcg
     3061 aatcatgttg tcctcctcgt tgcgattgcc gcgaacacgg tcgcgctctg cccgctatgt
     3121 ataatcgtac ggtcctgcgc atccaaggct tttccgacaa aaacagccta ccggcgcccg
     3181 gcggaaggga gtagagtcgg tcccgcatgg acccagcccc gacagcggcc ggccgccttt
     3241 tcgcgcgcgg cggcgcgccg gcgatcttcc tcgtcgccct gctcgcccgg ctggcggtcc
     3301 tgcttcccct ccacgcccac ccgctgtacg gcgttcccat tgtcgacgcg gcttcctacg
     3361 ccgaccaggc ccgggcgctg gcggccggcg cgccggcggg cgaggcgctg ttctggcagc
     3421 cgatcttcta cccgctctgg ctttccgtgg ttttccgcgc attcggcccc tcgctgctcg
     3481 cggcgcggat gctccaggcc gtgctgggcg cggcggcctg tgtcctggtc tactttctca
     3541 cgcgccggct gggcgggcgc ctcacggccg tcgcggcggg gctgatcctg gccttgtacg
     3601 ggcccgtgat cgccctggaa gccgacctgc tttcggaggg ctgggccgtg ttctggaccg
     3661 ccctgctgct ctggctgctc cagcgcgcgc agaccgagcc gcggcccggg acgctcgcgg
     3721 cgctgggcgt cgccggcggc ctggccgcgc tcacccggcc gacattcctg ccctttacgc
     3781 tcggcgcggg cctgtggctc accgccctcc gggtccggcg gtacggctgg aaggccggcc
     3841 tggacacggc cggcacgctg gcgatgggcc tgctggccgt cctcctgccg gcggcggcgt
     3901 tcggccgcca gcacacgggc cggttcaccg cgttcccctc cagctccggc ctgaaccttt
     3961 acctcggcaa caacccgcga tggatcgaaa cggtctccat ccgccccggc gtcgagtgga
     4021 tggagttgat ccgcgagccg cagcgcgcgg gcctttcgga catctgggat caatccgact
     4081 acttccggaa caaggtcgcc gggtacgccc gctcccagcc cggcgccttc gcggccgggc
     4141 tgctgcaaaa ggccgcccgc ttcctctgct cgcgggagat cccccgcaac cacgacctct
     4201 actcgctgcg cgaagccgcg cccgtcctga agccgctgct ctggaaggcc ggcgggttcg
     4261 gattcccctt cgggctgctc ctgcccctcg ccctgctcgg gttggccgcg cgtcgccggc
     4321 agctgggcgc ggcgtggctg ttccttctgc tgtattcgcc cgcggtgatc gcggtcttcg
     4381 tctccgaccg ctaccgcgcg cccgcggtcc cggcgctggc ggtcgccgcg gctctcggcc
     4441 tggcctggct ctgggagcag cgcgctaacc ggacgcgcct ggccggggcg ctcgcggcgc
     4501 tcgcggccgc ggccgcgctg accagcgtcg cgcgcgcgta tcccgaggag caactcgcgt
     4561 tcgaggcgga gcggaactgg gcggccggcc agcgcctggc cgcggaaggc cggcccgccg
     4621 aggccgaggc ctgtcttcgc cgcgcgacgg agatcgatcc gcggatggtg gacgcgtgga
     4681 acagcctggg cgtgctgctc gagcaggggg gccgccgcgc ggacgcgatc gacgcctatc
     4741 gccgcgccgt cgccctcgat ccgtccaacg agggcccgcg cttcaatctc gaggcggccc
     4801 ggcttccagc ttccggttcg tctgctccag ccgccgggcc atctccttga gcaggtcgaa
     4861 gacgatggcc gggttctcgc cgaggatgac ctcgaagttc ttctgcgaga tcgccgccaa
     4921 tcgcgttccg ggccccgacg cgacggcggt cgccgcgcgc ggcgcgccga ggagcatgga
     4981 catctcgccg aaatactcgc ccgggcccat cacccgcagt tcccggccgg cccgcgtcag
     5041 ccggacggaa ccctccagga cgtagaacat ctccgcgccc ggatcgccct cgcggaacag
     5101 cacctcgccg tccgcggccg cgcgcacgaa ccggcccagc aatacccggg aggtccgcgc
     5161 ttccggcccg ccggcgaaga gccggcgcag caggagggcg tcgcgccgct gggtcatggc
     5221 caccacctcg gcgccgaaga gcaggacggc gaaggtgtag taggcccaga ccaggagcag
     5281 gaagatcgcc ttgagcgagc cgaaggcata gccgtaattc ggattgtatt tcaggatcag
     5341 gccgaagagc gggcgcacga cggccaggag cgccgcggcg gcgagcgccc ccgccagcag
     5401 gtggggcagc cggatctttc cgggcgcgaa cacgcggtag aagaaggcca gcacgccgac
     5461 cgccagcgcg gcgctcccca gggcgcgcgc acaggccagc gccgcgcccg cccgcgcgag
     5521 cagccgcacc gggcccagcg acgcgtagac cttgccgccc gccatcagca ggaacagcag
     5581 gagcagcacg aggacggcgg acaggtcgag cagcttgctc cgcaggaaag aggcgcggtg
     5641 ggccgcgcgg aagatccttc gcacggcggc gcggatcgcg cccgcgaacg gcatcagcga
     5701 ccagaacagc agcacgacgc tgatcaggcc ccaggcgcgg ccgcgggcga cggacaggag
     5761 atccaccagc agcgcccggt tgaacgacgg gaagagacgc gccgccaggt cttcgacacc
     5821 ggcgaggacg gcctccgagg actggaggaa caggccgagc aggtagatca cgagcagcag
     5881 caggggcatg agagccagga acccgtagta ggccagcgtc gcggcgctcg ccatgtcgcg
     5941 gttggcggag aacgactgcc cggcctcgcg gaggaccagc cagagggagg agcccgccac
     6001 gcggagacgc tccggcgccc ggatgcggac gtcagcgctc atgcgccttc accgggtcca
     6061 cccggtattc ccggatgacg tcgagcgtct tgcgcttctt ttcgcgcggg cccggctcgg
     6121 cgggatagcc gaggctcaga atgatgtcca cgcgcgcgga gcgcgggagg cgcagcacct
     6181 tcttcacgcc ccgctcgttg aaccacccga gccagcaggt gcccaagccc tcctccgccg
     6241 cctgcagggc caggtgttcg cccgcgatgc cgatgtccac caggctgtac tggatgccgc
     6301 ggaactgccc gccgagctgc gccgcgtact tcgagcgctc cgtgacgatg acgatcagca
     6361 cgggggcctg ctcgacaaaa cggttcatcc ggtaaattcc cttgaacgcc tcgcccacga
     6421 gccggccgcg caggggctcg gaatccacca cgatgaattt ccagggctgg gagttgcagg
     6481 ccgacggggc cagccgggcc gcctcgaggc agcgctccag cgcctcgcgg ggcacgggcg
     6541 ccggcgcgta acggcgcgtg ctctccctcc gggccgccag ttcgagaaag gacatggcca
     6601 taacgtaact ccgcgccccg cagggtcaag cgggatcgcg ctccgaggcg atggttcgga
     6661 agagctgcag caacgcgcgc gacgcgggat ggtccggcgg catgcgctcc gggtgccact
     6721 gcacggccat cacggggatg ccgtccgcct cgacggcctc gacgatcccc gccggcgagc
     6781 gcgccgcgat cttcagtccg cggcccggcg ccgccggatc cacggcctgg tgatgcgcgc
     6841 tgttgacgtc cgtccgcggc cccagcacgg aggcaagacg gctccgcgct tccacctcca
     6901 ccggatgccc gctgtcccgg ccctccacct gcgcatgaac ctcgcgcgct gccgggcaga
     6961 tgtcggggat atgctggatc agccggccgc ccagcgcgac gccgacgacc tggatgccgc
     7021 ggcagatccc gaagacgggg cggcgggctt cgagggcggc gcggaccagc atgatctcga
     7081 atacgtcgcg cgctcgattc acggcatccg tgcgcggttg acgcgcggcg ccgtacaggt
     7141 ccgggtccac gtccccgccg ccggtcagca gcagggcttc gaacgacgcg ggcacggggt
     7201 cgcccggccg gacccactcc gccggaactc cggcggacgc cagccagcgc gcgtagcgcc
     7261 ggtccgcctc gtcccgcaac gtgctggaca ccagccatgt catgcccggg gcatccttga
     7321 cctttccggc atcctagcga atattccgcc gcatgaacat cgaatgcatc gtggtggggc
     7381 cgttcgaggt gaacagctac atcgctcacc ggggcgccgg cggcgaggcg ctggtgatcg
     7441 atccgggcgc cgacgcggat cttctgcgcg acgccctgcg ccgcctgcgc ctgcggcccg
     7501 cggcctacct gctgacccac ggccacgttg atcacgtgag cgccctcgcc gccctgcacc
     7561 gcgaatggcc ggccccggtg atcctgcacg aactcgacgc gccatgggcc ttcaccccgg
     7621 tgaacgaact cccgccgtac tatgccgccc cggccgcccc cgcgggcgcg ccctgcctcg
     7681 tcaaggacgg ggaggcccgg accgacggcc cgttccggta ccgcgtcatc gccacgccgg
     7741 gccacacgcc gggcggggtg gcgttccacc tgcccgacga gcgcgtgttg ttcgccggcg
     7801 atacgttgtt ccagggcacc gtcggccgga cggacctgcc gggaggcgac gcccgcgcgc
     7861 tggcgcgttc ggtcctgcag ctcggggaac tcccggacga cacccgcgtg ttccccgggc
     7921 acggacccgc gaccacgatc ggcgaggaga agcgctcgaa ttttttcatg caccgcgcgg
     7981 cgtcctgact gcgccgggcg ggatcaatcg ggcgtgacgg gattctcgcc ggccggcttc
     8041 tccggcgcgt cggcgggctc gcccttggcc acgggatgcc agtcgccggc atagccgagg
     8101 atgatgtcct gctcggccgt gcggatgagt tcgaaaacga ggttctggac ctgccggttg
     8161 ccgacgcgtc ccttatccat ggtcaggtgc acgtgggtga tcggccggtt gggcaggttg
     8221 accggcgtca cgagaaggtc gatcccctcg gccgaagccc gttccccgcc ctcggcgtcc
     8281 caccaggcga ggcgggcggc gcgcacttcc aacgccacgt gctcaaacca cgaggccgtg
     8341 ttggtcggcg ccccttcggc ggagggcgcg gccgggcccg cctcgttccc gtctcccggc
     8401 gcagacgacc cggcggcctc cggggccggc agttcgaacc cgccccactg cgcgacctgc
     8461 gcgcccagcg gttcgagcgc ggccggctcc catcggcccg aggaaccggg cgcgaaggtg
     8521 atagcccctc cgcgaatgac gacgcgctgg aacaggggcc cggggcggac caggccggac
     8581 agcgaccagc ccagcaccac ctcgtgcgcc cggatccccg gccggccgtt cttctcgctg
     8641 tcgcgggagg ccaggccctc cagccggagg ttcaggccgg gcgtggcctc cacccgcttg
     8701 agcgataccg gaaggccgag cgcgcgctcc aggcgatcct ccaccgagag gcggaagccc
     8761 tgcgtccggc tggccaggaa caagccgagg tagacgatgc cggccaggca gacgagcacg
     8821 ccgaggatgt gcgccacggg cccggacgag cccgggcggc gctcctcggt gatgcggtag
     8881 gctggatctt tgccccgtcc catgccgtct ctttcaactc ttcaaaagct ctgccgcgcg
     8941 gcggcgcgcc gccaacacgg cttccacgaa ggactcgaat acccctttct cccgcaggac
     9001 gttcagggcg gcctcggtcg tgcccccctt cgacgtgacc cgcgcccgca gctcggcggg
     9061 cggcaggccg gtttccttca tgagccgagc cgcgccctcg atggtggcca cggtgagatc
     9121 gcgcgccgtc ccggcatcga gttgcatctt ctccgccgcg tgcaacagga cctcggcgag
     9181 gaagaacacg taggccgggc cgctcccgct gaccgccgtg acggcgtcca tgtcggcttc
     9241 cttcacgcgg gccaccaggc cgacggacgc catgagcgcg cccgcggtcg ccaggtccgg
     9301 ctccgtcgcc catcgcccgc cgcacagggc cgaggccccg gcctggacca gggagggcgt
     9361 attgggcatg acgcggacga cgcgcgtgcc ctcgccgagc ccgtcctcga ggctcgtggt
     9421 ggggatgccg gcggcgatgg tcatgaccag ggccgtcttc gggatcgcgc cgcgcagttc
     9481 tccgagcacc tcggccatcg cctgcggctt cacggccagc accaccacgt ccgcacggcg
     9541 caccgcctcg cggttggcgg tgagggcgcg cacgccgaag tgcttttcaa agtattccct
     9601 ccgctcggga cgcacatcgg tcacgccgat cgcattccgg ggacaaagcc cgccgccgag
     9661 gatgcccttg acgagggcct cggccatgtt gcccgcgccg atgaaaacca cgttgccgac
     9721 gtccggcttc gccacgctca tgtgcgttcc ctttccccga aaagggccgt ccccacgcgg
     9781 acccacgtgg cgccctcctc gatcgcgatc tccaggtcat gggtcatgcc catcgacagt
     9841 tcgtccagcg cgaagccggt ttcgccgcgc caggcgtccc gcagttcccg cagccgcctg
     9901 aaccacggcc gggcctccgc cgggtcctcc gtgaacggcg gcatggtcat caagccccgg
     9961 acttccacgt tgtcgagttc attcgcccgg gccagcaggg ccggcacgtc cgcgggcgcg
    10021 aggccgaact tcgagctttc cccggagacg ttgacctcga ggagcacggg catgaccttg
    10081 cctgcctcgg cgctggcgcg gtccacggct tccagcagcc gcgcggaatc cacggaatga
    10141 atgtagtcga aaagctgggc cgccacggcg gccttgttgc gctggaggtg gccgacaaga
    10201 tgccacgaca gccggccggg acactgcggg atcttggcct gcgcctcctg gaccttgctc
    10261 tccccgaaca cggtcagccc gcactccacg gcctcgcgga cgcggtccgg cccgtgcgtc
    10321 ttggacacgg cgaccaggcg cgcgccgccc ggatcgcgcc cggcgcgcgc acacgccgac
    10381 tccatccgct cccgaacggc ctgcaaccgc tctgccaaac ccgagtccat gctgtccgcc
    10441 ttataccgga atttccaatg attggaaaaa agaatcgccg ggttttccaa tcattggaaa
    10501 aacatggtac cgtgccggca aacggaaacg ggaggagcgc atgagtcggc aggaaacgat
    10561 ttcgctggag cagggccggg cggaaggcca cgtctcgccg atcgggcccg tcaacctggt
    10621 gtggatcacc gcgccgaacg ggatgatcgg ctgcggcgcg ttcgacgtcg tcgggctgga
    10681 gcggttccgc tacgcggccg cccgcatgaa gggcgcgggc ggaaaggtca tcgcctcgat
    10741 cgaggacctg ctcgaaggcg aggtgcgcga ggccaacgcg ttcgcgcggg aaaaggggat
    10801 cgaggccggc ctgagcggcc gcgcggcgct ggcaaagctg ctcggttgat tcagcgttcg
    10861 aacgggtcgc gcgcgacggg ctggccgcag tacatgcagt agggccggcg cttttccatc
    10921 gggcggctgc acttcgggca gagcgagggc ggctcgggcg cgacgcggcc gtccagcttg
    10981 ccgtcgcgac ggtcgatctc gtgaatccgc cggagcagtt cctcctcggg aagatcgaat
    11041 ttttccgcga ggatgctcca gagcgcctcc gcgatcatca gcaaccgctc cacctccgcg
    11101 cggagcgtct ggatctcgat tcggtcccgg acggccccgg cgtccgtccg ggcgacggcg
    11161 tagtccgggt tctgaaagac ggatcgaaag aaatggtccg gcatgctggt ctcctttcgg
    11221 cggcgcccgc cgtcggcttt ctaccgcgaa ccggccaccg gcttcaagac cgcgtaggtg
    11281 ggcaacccga tggccccgaa ccggtccagg atgtccgagg ccggcgggtc gccgggctgc
    11341 tcggcctgga acttgaccac gaggtacccc gccaggcggg cggcgacggc aggatccttg
    11401 aacgtggtct tttccatcac gaggcagttc ttgcaccagg tcgcccagaa atcgaggaag
    11461 acgggccggc cttcgcgcgc ggcctcgtcc agggccgcgg ccaggccttc ggccgaatcc
    11521 accttgcgca ggttcgaggc gccggttttc acggccggcg gcggcggggg cggacggaac
    11581 aggcggtacg ccgtatagcc gtagtacagc gccagcagca ggataagcac gccgaacagg
    11641 ttgcggacca cggtcatcca gcggccgggc ttgggcagga acgacaggcc cgcgcccgcc
    11701 agcggccacg gcagcgccat cccgaggccg agcacgaacg gcagcaggag gcccagcgcg
    11761 atgccgcgcc cgtagagatg gccggccagc acgagcacgg agatcagcac cggcgccacg
    11821 cacgccccgg ccagcagcgc ggaaacggcg cccatgacca gggccacggt gtacccgccc
    11881 cgcctcccct ccgccccgcc cccctggaac cggctcaagt tgatctcgaa cacgtcaaac
    11941 atggctagcg acagcgccac gaacagcccg gccatggccg cgttgaacca gggcgacgcg
    12001 ttgagcgcgc cgaaccgcgc gccggtcagc acgacgatca cgcctaggag gccgtaggcg
    12061 agcgccatgc ccaggccgta cacgccgccc agcgcgaaac cgcgccggcg ggaaccggcg
    12121 cgcgccccgg ccccgatgat ggccaggttg acggggatca tgggcaacac gcacggcgtg
    12181 aggttgagcg ccacgccgcc ggcgaggatc agcgccaggg tggcgagcca gcccagcttc
    12241 tgccagcgcg ccacgagccc gtcgccggcc gccgcaccgg attccgcctg cccgaggaac
    12301 tccagcagcg cctccggttt cacgtaaccc gccgccgtgc ccgccacgct gaaccgctcg
    12361 agcgccgcgc gccagtcgcc ggccgccggc gacgcagggc cgggcgcgac cgggagggcc
    12421 gggctatccc ccgcagcggc gcccacgggg acgatccgct tttcggggaa ataacagacg
    12481 gacgcgtcgc agccctggaa ggagacttcg acttcgagga cgcccgcggc cacgggcaac
    12541 tggtacaacc ggtcgtgcgg cttgtccaag acagcctttt ccccgccgct gtgcttgtcc
    12601 cggacccggg tcggcgtcgg cgcttccgcc aggaccagtt cggcgccggc gcgggcggcc
    12661 acgtgaatcg agtccgcgta gagatagtgg cccggcggat actggaaatg gacgcggacg
    12721 tgcggctgcc cctcccgctg atcgaaggac gcggtaacga caaaaggact ctcggcggcc
    12781 agcgcggtcc cggccgacag gagcaacccc gcgagccgat ggaaccgaat ccccctcatc
    12841 gcgcggcttt ctacggcgag gccggggcgc ctttagtttc cgcgaggagg gccgagatgt
    12901 ccgcccgcaa ctgctccacg ggaaccacgc ccagatagct cttgcgcacc ttgccctggg
    12961 cgtcgagcag gaacttggtg ggcacggcgc ggatgccgcc ataggcggcc tggatgtcag
    13021 gcccggccaa tcccacggga tagggaatct cgaaccggcc cacggcctgg gccaccgact
    13081 cggcggcgcc ctggtccacg gtcatgccga cgaggacgaa tccctggtcc ttgagttcct
    13141 cgaagaggcg ggcgagttcg ggcagttcgg agcggcacgg cgggcaccac gtggcccaga
    13201 agtccagcag tgcgacccgg ccgcggaagg agttcaggtc gatggactca ccggcggcct
    13261 tgagcgacgg gatgaaaaag gcggaggcgg cgggcccgga gggggacacc tcgggttgcg
    13321 cggcccggtc ttcgccggat gggccgcatc ccgccagcgc aaccgctacg aaggccgccc
    13381 atccctttat ccgtggcctg aaattcatga tacgctctcc gtttacccgg taaggatacg
    13441 ttatccggcc ggggattcaa gaacctcggc ggcggacggg gccaggagta taacaatacc
    13501 attcacactc gtgaattaat ttattacctt cgccacaggg gaaacggcgg cagggagcgc
    13561 cgaaggcaac ggatcctaaa atggcggccc ccgcaatatc ctgcttgcgc aagggcgggc
    13621 gcatttctat agtgcgcgtg ggttaacgcg tttgacgaga caagcaaggg tgaacacgtg
    13681 aagaagagga cccgctgggg cccgccggga tggccgtggg agctggaatt gctggccttg
    13741 gtctttgtcg tcacggccat cgtcgtcccc tactacctcc aggcgcagac gacggagtcc
    13801 ccccaactct cgatcctggc ccaggccgaa caggcccagg cttcccagcg cggaagctgg
    13861 caggacctcc aggaattcat ctgggccgtg atcatcgtcg ggctgtacct ggcgcacctt
    13921 gtggtcgcca gctccagcat cgactacatg tccacgccct tcacccacct gttttccccg
    13981 ctcttatttt ccatgatcac gtactaccgg ctgtacacgc tggtgaaaga ttcgccgacc
    14041 gccacggcga tcgtgagcgg ttcgccgctg gagatcgccc tctgggtcct cggcgtcctc
    14101 gtcatcacct tcatggtcgc ccgcatccgg atggcccgct acatgctcaa cttcaagcac
    14161 gtggactggg acctcacgac gcccaccctc ttcgatcggt ccttctggga actggccgtg
    14221 tacctccggc cgctcatcta tcccccgcgc ctctaccgcg cctgccccaa cggcatcctg
    14281 atcgagggct gggtgtatgc catgcccatc cccttcgaga ccatccattc cgtggacgcc
    14341 gtccaggggg ccggcttcct gtcgtccggt tattgcctcg cgacctcggc gaaatccctg
    14401 gtccggctgc agatctcgga aaaggccgag cccctgctga tctccccgaa ggaccgcgcc
    14461 gagttcgtgc gctactgcga gcagcggttg agcacgacgc gcctgatcaa gatcccgtcc
    14521 gagacgcggc acggcgccgc cggcaccacg acccgcggct aggggccgcc cgccgcccat
    14581 gaacccgatc ggcgacagcc gcccgcagga ggcggcgcgc gagatggtcc gcacgctccg
    14641 cgccgccgga ttcgacgcct actgggccgg cggctgcgtc cgcgaccgcc tgatgggccg
    14701 gcccgccaag gacatcgaca tcgccaccag cgcgcggccg gaacaggtcc aggccctctt
    14761 cccgaagacc ctcgagatcg gcaaggcctt cggcgtgatc caggtgctcc gggacggctt
    14821 cgcgttcgag gtcgccacgt tccgccacga ccggccctac cgcgacgggc ggcatcccga
    14881 cggcgtggcc ttcaccagcg ccgaggagga cgcgaagcgc cgcgacttca ccgtcaacgg
    14941 gctattcttc gacccgatcg aggaccgcgt gctcgacttc gtcggcgggc aggacgacct
    15001 gcagcgccgc ctcatccggg cgatcggcga tccggaagcc cgcttccgcg aggaccacct
    15061 tcgcctcctg cgggccgccc ggttcgcgtc ggcgctggag ttcgacatcg aaccggccac
    15121 cgcagaggcc gtccgccggc tcgctccgct gctggcggac gtcagcgcag agcgcgtcca
    15181 gcaggagctg acgcggctcc tggtcgagtc cccgcgcgcg ggtcggggcc tggccctctt
    15241 gcgcgacctg ggcctgctgg ccgtcgtgct cccggaagcc gagcggctcg tcgggcagga
    15301 gcagccgccg gcttttcatc cggaaggcga cgtcttcacg cacacggtcc tgatgctgga
    15361 cgccatgaag gatgccacgc cggaactggc ctggagcgtg ctgctccacg acatcggcaa
    15421 accgctcacg gcgacgccga cgatcgagcc ggacgggtcc acccggatcc gtttcaacga
    15481 gcacgacagc gtcggcgccg atctcgccga gcagatcctg cggcggctca agatgccgaa
    15541 cgacctggtg gacgccgtcc ggcagggcgt ggcgaatcac atgcggttca tcaacgtccc
    15601 gcggatgcgc cggtccaagc tgcgccagat ggccggcgcg ccgacgttcc cgatggaact
    15661 cgaactgcac cggctggact gcctggccag ccacagcgac ttgagcaact acgagttcat
    15721 gcgccggttc atggaggaac tgaaaaacga gccggtcctc ccgccgccgt ggattcgcgg
    15781 c
//
LOCUS       DAOOTK010000072        15650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2553_length_15650_cov_5.057134, whole genome shotgun sequence.
ACCESSION   DAOOTK010000072 DAOOTK010000000
VERSION     DAOOTK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15650
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_15650_cov_5.057134"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..2301
                     /locus_tag="PK634_08980"
     CDS             106..2301
                     /locus_tag="PK634_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97908.1"
                     /translation="MTDPETKPPNKLARLGLPPLLLALAVALVYGHTGRSPFLLDDTF
                     NIVENPAIRRLWPLAEALDPPPVDMTFYTRPFINLTMCVDYALGGLNPETYHRTNFLF
                     HWAAALALFGLARRTLRRTGMEDGAAWTVGLAAALVWAVHPLNTAAVSYLSQRGELGV
                     GLFLFLMLYGLNRAAAPGAVLGGVFWLAAAVFSCLLGMGSKESMAAAPLLALAYDRIF
                     LSATWKEALRRRGPFYVALALTWAWPLSRILTFSPHLPEDGFDPLPFRHYALTQAWGL
                     SRMLGLAIWPRGLVFEYGTGLVSSPADVWPQLLFMATALALSAWAVLRRPRAGFAALL
                     FLAVMAPSSILPITGQPVAEHRLYAALAAPAALAAWGLWRLLRERPWAWRALVAAWIA
                     ALGLASRQRNELYNDPLRLWSDTLAKRPSNDRAWGGLGQALDARGRHAEALRAYELAL
                     RRKDSRITRSSMGTTLYNLGRYPEALRQFQASLRLHASDAKALHWLGLTLAALNRHEE
                     AIEAQRRALSLQPDYVAACFQAARSLVALRYLEEGLAAYREAARLQPTALEAHSRIAL
                     TLLALGRADEARAALLDGARASGAPARAYFEFSNALLAQGHAAEALDMARRHLELEPA
                     HPGSLNNAAWILATSTNTALLDGDEAVRLAQRAMAQSGATVPALWATLAAAQAEAGRF
                     DEAAASGERALALAREANATNLLASYALRLEAYRGRRPWREGGHDDAAK"
     gene            2311..4470
                     /locus_tag="PK634_08985"
     CDS             2311..4470
                     /locus_tag="PK634_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97909.1"
                     /translation="MNRAAARWMEAFLLVLAVVLVYGNTLDHPFVLDDNHAIVNNPAI
                     RRLRPLTAALNPPPADVSFCSRPLINLTMCVDYARGRTAPRPYRRTNLLLHAAATLAL
                     WGLARRTLRASSVDESAARLAGLGIALLWAVHPLNTAAVNYLTQRGELGVGLFLFLTL
                     YGLNRAAAPGARSRSWLAASVLFCLLGMGSKECMAAAPVLAMAYDRIYLSPSWREVFR
                     RRGPFYVALALTWAWPLWRILFYSQHIPSGGYDLAEHWHYLLTQAWGLARMVRLAAWP
                     APLVFYYGEPLVTSLADVWPQALFVAVLLGLTAWALLRRPRLGFPALCFFAILAPSSS
                     FLPIPGQPIAEHRLYAPLAALLALAVPGLWFAGRRAGDRGARIATALALAWAVALGWA
                     AHRRNAQYRDLLVLWQDTVAKRPDSDVSWATLASVLNDRNRHEEAARAFEQALRCEEN
                     NYIVHANLGVTLYTLGRYEDALHHLEESLRLHPSGAEGHHWRGLALSALGQREEALAA
                     QQRALVLKPDYLPARFHLANELAGLGRSAEAADEFRRVIRQKPTALEGYGRLAALLLD
                     LGRVDEAHALLAEGARASGAPARAYFEFSSALLAGGHEAEGLDMARRHLALEPDHPGS
                     LNNAAWILATSTNAARRDGAEAVRLARRAAEQFPEPDAALWSTLAAAQAEAGQFPEAV
                     VSAGRALEGALKQNDTNLAARCRARLETYARRQPWRE"
     gene            4531..6711
                     /locus_tag="PK634_08990"
     CDS             4531..6711
                     /locus_tag="PK634_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97910.1"
                     /translation="MDNPSTPPAPFPWPRLATALALGVLALVVFLPAAGFDFLNLDDL
                     NYVADNEHVNTGLSWANVKWAFSSAHENWWLPLLWLSYMADSELFGPGPFGYHLVNVL
                     LHALNTALLFWALWRMTGSAARSAFVAALFAVHPQRVEAVAWITSRKDVLSGLFYLLS
                     LLAYLRCVERPTLARRLLLPVLMLLGLMSKAILITLPPVLLLLDYWPLKRAGDPLDRR
                     EWKKWGALFMEKLPLVMLALVFVVVNLHTHTTGRGLLLPFPLSIRLGLMPPNYWAFLG
                     KLFWPARLAIVYPEADLVNGDVTAAALAGLAAATGLVLRLRRRQPWLLVGWSWFLVAL
                     LPVIRGLRMGIAGMADRFTYIPGIGLFILLAWGVSEALPPAVGKKALLGGLAAAAVAA
                     CLVATRQYLSCWKDSETLFPRSIAVTKDNYVLWLNLAGAHQRKGRLDEEIECLRKGLD
                     ANPLCATLHVLLGQAYDRKGRPAAALAEFEQAARLALPFEAHNHAVVGERFARAGRYE
                     EAIRHLDVSLQADPASIDTRRNLGLAHLFAGHPQEALSCFQEVIRRAPQDDRAYFLMG
                     KILLSLQRGEEAIRALRAAAERAPDKVETRSLLAVALGRAGRMKEATALLRAALSTRP
                     NSPELLNNLAWLLAARPGRAGHDPKAALAYALRAAEITGRRNPDILDTLALAQAADGQ
                     YDAAVETLQEAERKAEAEGIKDFPGRIALRIEAYRAGRPWQEEP"
     gene            6988..7623
                     /locus_tag="PK634_08995"
     CDS             6988..7623
                     /locus_tag="PK634_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW97911.1"
                     /translation="MKKLLIALMVLGMAAVSQADVIANYTFAGSAATSSDTSALSTAG
                     SFSISAGTWTYATSSSSSWAGQGGEIPVADRATWGTAATSFETQTTYWYFTVAVDANY
                     QLSITGLDFLGQRTVAGPASFGWDVNGTLQESWTPGVTTVNSYSSAQTEGPLTGTVTI
                     GIRGWSATSTGNFRVDNVVLNGTITAIPEPGVLALLGLGLGALFYVRRNRK"
     gene            complement(7753..8049)
                     /locus_tag="PK634_09000"
     CDS             complement(7753..8049)
                     /locus_tag="PK634_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97912.1"
                     /translation="MVEQVEAAFPGHGVDVRSFYSLEEEGPKAASRLADTRTEGTFFI
                     FMLQDISPGKAYDVQHTVTLETASWSNRQVVDTWQPAVTIQEEMTESSGFYRAR"
     gene            complement(8320..10158)
                     /locus_tag="PK634_09005"
     CDS             complement(8320..10158)
                     /locus_tag="PK634_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97913.1"
                     /translation="MRITLPRRLGALGLLLATGLAYSNALDGPFLMDDQLSIVENTAI
                     RQWWPLAAALNPPPANVTFFTRPFVNLTMCVDYALGGLDPRGFHRTSLLLHLGATLAL
                     FGLARRTFEAAGRTEAEARRLGFASALLWSVHPLLTSAVNYLSQRSEVAMGLFLFLML
                     YGLNRAVREAAGGPATSRARFWFAVSALACLLGMGSKESMAAAPLLALAYDRIFLCAS
                     WRDLHRQRGLYYLALALTWLWPLGRHLAYSQYAPDLSVPVGDYWHYLLTQAWGLTRML
                     RLFLWPAPLIFYYGTQMVERASDVWPQLCLMAGLGLATAWALARHPRIGYLGLFFFAV
                     LAPSSSFIPVAGQPIAEHRLYAPLAVLAVLSVVVLDRALRLLPARYARGGFIAVLLAW
                     AATLGTAARERNEDYRDLLCLWRDTIDKRPQNGRAHTEYARVLRLLGRPEEGRAHLER
                     VFANPPTEPGDLIHLGNALVSFGKLDEATRCFRLVLNSNPDLVLVWNSLGTTLVRLDR
                     PDEAVAAFEEALRWSPTYTEPHYNLGNILFQRKQYDAALEHYRQAARGRPDVALNWFN
                     LGVAHAQLEQWPDAERCVRRALELDPSLPNGRETLRRIRAAGPGGG"
     gene            complement(10155..12005)
                     /locus_tag="PK634_09010"
     CDS             complement(10155..12005)
                     /locus_tag="PK634_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW97914.1"
                     /translation="MGETEAKGSSRASARSWWIGLGLAVAAVLAQGNGLHGPFVLDDT
                     TNFVENPSAWRPWPLSAALNPAPGGGINLATRPVLNLSYCVDHAIWGFKPSGYRATNV
                     LLHIAATLALFGLVRRTAEKMGRGDSLGWGAAAALLWTVHPLLTTAVKYLSQRGELLV
                     GLFLFVMLYALNRAAEQRASGPGGTARAAGWLAASVFACLLGMGSKENMAAAPFLALA
                     YDRIFLSPSWKEVVRRRWGYYLALALTWIWPVWRIAHYSPHMPEGGFPLFVYWRYLLT
                     QAWGLVRMVRLAAWPSPLIFDYGVGLFDRIVYVWPHVVLCIGLLAVTGWALWRHPRLG
                     FLGLFFLGVLAPSSSLMPITGQPVAEHRMYAPLAALLAARVNLKPRALRAALLGAWVV
                     SLGIASRERNKVYASEPVLWGDTVQKRPRNLRAHAEYGRSLCEAGQFENGLAHLELIK
                     QDPFAKAGDLANLGLALAHFKRFDEAMACYRNAIRLSPESAVSYGNMGRTLVDMARTD
                     EAVAAFEKALELRPDYAEAHYNLANLLFKDGRPRDALAHYQKAAEYRPGVALNWFNLG
                     VTWAALGNFGEAERCVNRAWALDPMLPGAWPTLQRIRAELQRGAGPAGGP"
     gene            12138..12521
                     /locus_tag="PK634_09015"
     CDS             12138..12521
                     /locus_tag="PK634_09015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOW97915.1"
                     /translation="MIESIAGRERRRHRRLACERPASIHVVHCPDDPRLERRSLYGMV
                     RNISAGGLKFTCPQSLPPGTLVHLDVAMPERGCLFKLAARTVRSTPRTDRFEVGAALE
                     DSFFRRRDPWARMVFDALRELNDPV"
     gene            complement(12537..13133)
                     /locus_tag="PK634_09020"
     CDS             complement(12537..13133)
                     /locus_tag="PK634_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97916.1"
                     /translation="MMKAGGRIAWVLVLAVGVCRAAEPAQATPPEPGFVEFVESEIIP
                     MMVETGQAPAAASSPWSEPAAAEPMAPPAGETEAAEPEPAPARRDPFWPVGYAPPPPA
                     RPSEAAASTAGAETVSAVLQWDEALKTVQVQGIMKTGPETHVAMVNGQVVGVGDTVSV
                     RFGGREYRWTVAGVGVEGVSFKRVEKEAAPADESESLP"
     gene            13506..13943
                     /locus_tag="PK634_09025"
     CDS             13506..13943
                     /locus_tag="PK634_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOW97917.1"
                     /translation="MRKTETQRRGRSGFTLVEVMIAVVLVLLALGGAYTLIVHSARVS
                     RAARDHYVAVNLAKNHLERARNFRYADLYLLAEDNLVMNENGGPDNRGRFRRTTVVDD
                     DYATGATEITVTVQVRNRRTGEFGEEKEQISSLFTEYLTPEIE"
     gene            13940..>15650
                     /locus_tag="PK634_09030"
     CDS             13940..>15650
                     /locus_tag="PK634_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOW97918.1"
                     /translation="MSTRESRGGFSLTELLVAVLILVITMAGAIAGWLFVVRGERMNS
                     IQQKLDMDVRKSLERLKAEMRLSTMDEVYFYPAGAGPYTAISFPMARDDDADGLVELD
                     EDDHIIWDQTVVYHIWQSTPHQLRRTVFDPRNNSLTPGQRQEQLNAVVTAGSGAGTHN
                     GANASTTVLFENLFTWTILGKGAVFDAYHPTLARMVGVNLGSFLLSPGAHAYKFSVID
                     KNPSSTGYKIGLDSLIVSPSGLEREGEVQTVSAQSGATASADYRSAGGWSGNYHLLFP
                     ATAVGNSMTLSMENDRWEETNFRGTGSLCDNTIVVFDDTLTPKDFVARLQPPNTTWLA
                     QDQTLDLNSGSTTNGFLQGCAVRVLVRGGSQPDGGAILNSGPFNFALFYAAAEDPLQI
                     TKAYIAEAASSFGYSPDILPETSRQLYFGGSEQVNISEGSYALGFLGSGQSLDIQNDR
                     SYIVSFLVTAAESERADARTWTELHPATATNPAPPGCYVLRGGTNLPDDVLTQATWAG
                     YPDFETHEKLYAVERVYVLAPSNGTFTSQIVDTQLSAPAYQTMTWNSVKPSGTSLGLK
                     IRAG"
BASE COUNT         2456 a         5451 c         5338 g         2405 t
ORIGIN      
        1 cggggcgaca ccaacctggc ggcccgctgc cggctccgcc tcgacgccta ccggaacggc
       61 cggccctgga gggaatgacg aagcctgcgc gccgcgagcc ctgccatgac tgacccggaa
      121 acgaaaccgc cgaacaagct cgcccggctc gggttgccgc ccctgctgct ggctcttgcc
      181 gtggccctcg tgtatgggca taccgggcgc agcccgttcc tgctggacga cacgttcaac
      241 atcgtcgaga accccgccat ccgacggctt tggccgctgg cggaggccct cgatccgccg
      301 ccggtggaca tgacattcta cacgcgtccg ttcatcaacc tgaccatgtg cgtggactat
      361 gcgctcggcg ggctgaaccc cgagacctat caccggacca atttcctgtt tcactgggcc
      421 gcggcgctgg cgctgttcgg gctcgcccgc cgcaccctcc ggcgcacggg catggaggac
      481 ggcgcggcct ggacggtggg gttggccgcc gccctggtct gggcagtcca cccgctcaac
      541 accgccgcgg tgagttacct ttcccagcgc ggcgaactgg gcgtgggact gttcctgttt
      601 ctcatgctgt acggcctgaa ccgggccgcg gcgcccggcg ccgtcctcgg cggcgtcttc
      661 tggctggcgg ccgccgtctt ttcgtgcctg ctcggcatgg gcagcaagga atccatggcc
      721 gcggccccgc tgctggcgct ggcctacgac cggattttcc tctccgcgac atggaaggaa
      781 gcgctccgcc ggcgcggacc gttctacgtc gcgctggcgc tgacttgggc ctggccgctc
      841 tcgcgcatcc tgacgttttc cccgcatctg ccggaggacg gcttcgaccc gctccccttc
      901 cggcattatg ccctgacgca ggcctggggc ctctcgcgca tgcttgggct ggcgatctgg
      961 cctcgggggc tggttttcga gtatggcacc gggctggtga gcagtccggc cgatgtctgg
     1021 ccgcagctgc tcttcatggc gacggcgctg gcgctctccg cgtgggcggt cctccgccgc
     1081 ccccgcgccg gcttcgcagc gctgctgttc ctcgcggtca tggcgccgtc gtccatcctg
     1141 ccgatcaccg gccagccggt cgccgagcac cggctgtacg cggcgcttgc ggcgcccgcg
     1201 gccctggccg cgtggggcct gtggcggctg ttgcgggaac ggccgtgggc ctggcgggcg
     1261 ctcgtcgcgg cctggatcgc ggccctgggt ctcgccagcc gccagcgcaa cgagctgtac
     1321 aacgatccgc tgcggctctg gagtgacacg ctcgccaagc ggccctccaa cgaccgggcc
     1381 tggggcgggc tgggccaggc gctggacgcg cgggggcgcc acgcggaggc cctgcgcgcc
     1441 tacgaactgg ccctgcgccg gaaggacagc cgtatcacgc gctcgagcat ggggaccacg
     1501 ctgtacaacc tcggccgcta tccggaagcc ctccggcagt tccaggcctc gttacgcctc
     1561 cacgcctcgg acgccaaggc cctgcactgg ctcggcctga cgctggccgc gctcaaccgg
     1621 catgaggagg cgatcgaagc gcagcgacgc gccctctcgc tgcagcccga ttacgtcgcg
     1681 gcctgcttcc aagcggcccg cagcctcgtg gcgctgcgct atctcgaaga gggcctggcc
     1741 gcgtaccgcg aagccgcgcg cctccagccg acggcgctgg aagcgcacag ccggatcgcg
     1801 ctcaccctcc tggccctcgg ccgcgccgac gaggcccgcg cggcgctgct cgacggcgcc
     1861 cgggccagcg gcgcgccggc ccgggcgtat tttgagtttt cgaacgcgct gctcgcgcag
     1921 ggccatgccg ccgaggcgct cgatatggcg cggcgccacc tggaactgga accggcgcat
     1981 cccggctcgc tcaacaacgc cgcctggatc ctggcgactt ccacgaacac ggcgctgctc
     2041 gacggcgacg aggccgtccg gctggcgcaa cgcgcgatgg cgcagtccgg ggcgaccgtc
     2101 ccggcgctat gggccacgct ggcggccgcc caggccgagg ccggccggtt cgacgaggcc
     2161 gccgcgtccg gcgagcgcgc cctggcgctg gcccgggagg cgaacgcgac gaatctcctc
     2221 gcgagctatg cgctccgcct cgaggcgtac cgcggccgcc ggccctggcg ggaagggggc
     2281 cacgacgatg cggcgaagtg aggtcccgcg atgaatcgcg ccgccgcccg atggatggag
     2341 gccttccttc tcgtgctggc cgtcgttctc gtttacggca acacgctgga ccatcctttt
     2401 gtcctggacg acaaccacgc catcgtgaac aacccggcca tccgccggct ccggccgctg
     2461 accgcggccc tgaatccgcc gcccgcggac gtcagcttct gctcgcgccc cctgatcaac
     2521 ctgaccatgt gcgtggatta cgcgcgcggc aggacggccc cgcggcccta tcgccgcacc
     2581 aacctcctgc tgcacgcggc ggccacgctg gcgctctggg gcctggcgcg gcggacgctt
     2641 cgcgcatcgt cggtcgacga atccgcggcg cggctcgcgg gcctgggcat cgcgctgctc
     2701 tgggccgtcc acccgctgaa caccgccgcc gtgaattacc tgacccagcg cggcgaactg
     2761 ggagtcggcc tgttcctgtt cctgacgctg tacggactga accgcgccgc ggcgcccggc
     2821 gcgcgatccc ggtcctggct cgcggcctcc gttttgttct gcctcctcgg catgggcagc
     2881 aaggaatgca tggcggcggc gcccgtcctg gcgatggcct acgaccgcat ttacctgtcg
     2941 ccctcgtggc gggaggtgtt ccgccggcgc ggaccgttct acgtcgcgct ggcgctgacc
     3001 tgggcctggc cgctctggcg catcctgttc tattcccagc acatcccgag cggaggctat
     3061 gacctcgccg agcactggca ttacctcctg acccaggcgt ggggcctcgc gcgcatggtg
     3121 cggctcgcgg cctggcccgc ccccctggtc ttctactacg gcgagccgct ggtgacatcc
     3181 ctggccgacg tctggccgca ggcgcttttc gtggcggtcc tgctcgggct cacggcgtgg
     3241 gcgctgctgc gccgtccgcg cctcggcttc ccggcgctgt gctttttcgc gatcctcgcg
     3301 ccctcctcca gtttcctccc gatccccggc cagccgatcg ccgagcaccg gctctatgcg
     3361 ccgctcgccg cgctcctggc gctcgccgtg ccgggcctgt ggttcgccgg aagacgggct
     3421 ggcgaccggg gggcgcggat cgcgacggcg ctcgcgctcg cctgggccgt cgccctgggg
     3481 tgggcggccc accggcgcaa cgcccagtac cgggacctcc tggtcctctg gcaggacacc
     3541 gtcgccaagc gtcccgacag cgacgtctcc tgggccacgc tggccagcgt cctgaacgac
     3601 cggaaccgcc acgaagaggc cgcgcgggcc tttgagcagg cgctccgctg cgaggagaac
     3661 aactacatcg tgcacgccaa cctgggcgtc accctgtaca cgctcggccg atacgaggac
     3721 gcgcttcatc acctcgagga gtccctgcgg ctccatccgt cgggcgccga aggccaccac
     3781 tggaggggcc tggccctctc ggcgctcggg cagcgcgagg aggccctcgc cgcgcagcag
     3841 cgggccctgg ttctgaagcc ggattacctc cccgcccgct ttcacctggc gaatgaactg
     3901 gcggggctcg ggcgctccgc ggaagcggcg gacgagttcc ggcgggtgat ccggcagaag
     3961 ccgaccgccc tcgaaggata cggccggctc gccgcgctgc tcctggacct gggccgcgtg
     4021 gacgaagcgc acgccctgct ggcggaaggc gcgcgggcat ccggcgcgcc ggcgcgggcc
     4081 tatttcgagt tttccagcgc gctcctggcc gggggccacg aggccgaagg cctcgacatg
     4141 gcgcggcggc acctggcgct ggagcccgat catcccggca gcctgaacaa cgcggcctgg
     4201 atcctggcca cttccaccaa cgcggcgcgg cgcgacggcg cggaagcggt ccgcctcgcg
     4261 cggcgcgcgg cggagcaatt cccggagccg gacgccgctt tgtggagcac gctcgcggcc
     4321 gcccaggccg aggccgggca gtttccagag gccgtcgttt ccgccgggcg cgccctcgaa
     4381 ggggcgctga agcagaacga caccaacctc gccgcgcgct gccgcgcccg cctggaaacg
     4441 tacgcccggc gccagccctg gcgggagtaa acggcggcgt tcttttcctt tcccaacgcc
     4501 ggccaaagcg gctaacgttt catcatggat atggacaacc catcgacgcc cccggccccc
     4561 ttcccgtggc cccgcctggc gacggcgctg gcgctgggcg tgttggcgct cgtcgtgttt
     4621 ctccccgccg ccggcttcga ttttcttaac ctggacgacc tgaactacgt cgccgacaac
     4681 gagcacgtca acacggggct ctcctgggcc aacgtgaagt gggctttctc ctcggcgcac
     4741 gagaactggt ggctgccgct cctgtggctg tcgtacatgg cggacagcga actgttcggc
     4801 ccgggcccct tcggttatca cctcgtcaac gtcctgctgc atgccctcaa caccgccctg
     4861 cttttctggg cgctctggcg gatgaccggc tcggcggccc gcagcgcctt cgtggcggcg
     4921 ctgttcgccg tgcacccgca gcgggtcgag gccgtcgcgt ggatcacgtc gcgcaaggac
     4981 gtgttgagcg ggctgttcta cctgctctcg ctgctggcct acctgcggtg cgtggagcgg
     5041 ccgaccctgg cccgccggct gctgctgccg gtcctgatgc tgctgggcct gatgtcgaag
     5101 gccatcctga tcacgctgcc gcccgtgctg ctgctgctcg actactggcc gctgaagcgc
     5161 gcgggcgatc cgctggaccg gcgcgagtgg aagaagtggg gcgccttgtt catggagaag
     5221 ctgccgctgg tcatgctggc cctggtcttc gtggtcgtca acctgcacac gcacacgacc
     5281 ggccgcggcc tgctgctgcc gtttccgctt tccatccgcc tgggcctgat gccgccgaac
     5341 tactgggcgt tcctgggcaa gctgttctgg cccgcgcggc tggccatcgt gtatccggaa
     5401 gcagacctcg tgaacggcga cgtgaccgcg gccgcgctcg ccggactcgc ggcggcgacg
     5461 ggcctggtct tacgccttcg ccggcgtcag ccatggctgc tcgtgggctg gtcctggttc
     5521 ctggtcgcgc tgctgcccgt catccgcggc ctgcggatgg gcatcgcggg aatggcggac
     5581 cggttcacct acatccccgg gatcggcctg ttcatcctgc tcgcgtgggg cgtatcggag
     5641 gccctgccgc cggccgtcgg gaagaaggcg ctgctcggcg ggctggccgc cgcggccgtc
     5701 gccgcctgcc tcgtcgctac gaggcagtat ctttcctgtt ggaaggattc ggagaccctg
     5761 ttcccgcgct ccatcgccgt gacgaaagac aactacgtcc tctggctgaa cctcgccggc
     5821 gcccaccagc ggaaggggcg cctcgacgag gaaatcgaat gcctgcggaa gggactggac
     5881 gcgaatccgc tctgcgccac gctgcatgtc ctgctgggcc aggcgtacga ccggaagggg
     5941 cgaccggcgg cggccctggc ggaattcgaa caggccgcgc gactcgcgct gccgttcgaa
     6001 gctcacaacc acgcggtcgt tggagaacgc ttcgcgcggg cgggccggta cgaggaagcg
     6061 atccggcacc tcgacgtatc gctccaggcg gaccccgcgt ccatcgacac acggagaaac
     6121 ctggggctgg cgcacctgtt cgcgggacac ccgcaggagg cgttgtcctg tttccaggag
     6181 gtcatccggc gcgcgccgca agacgaccgc gcctacttct tgatgggcaa gattctcctt
     6241 tcgctccagc gcggcgagga agcgatccgg gccctgcgcg ccgccgccga gcgggcgccc
     6301 gacaaggtcg aaacccggag cctgctcgcg gtcgcgctcg gccgcgccgg gcggatgaag
     6361 gaagcgaccg cgctgctgcg cgccgcgctc tcgacgcggc cgaactcgcc cgaactgctc
     6421 aacaacctgg cctggctgct agcggcccgg cccggccgcg ctggccacga cccgaaagcc
     6481 gcgctggcgt atgccctgcg cgcggccgaa atcaccggtc ggaggaaccc ggacatcctc
     6541 gacacgctgg ccctggccca ggccgcggac ggccagtatg acgcagccgt ggaaaccctc
     6601 caggaagcgg agcggaaagc cgaggctgaa ggcatcaagg attttcccgg acgaatcgct
     6661 ttacggatcg aggcctatcg cgctggtcgg ccatggcagg aggaaccgtg agcctgatcc
     6721 gtccgcgtaa tattctctat gaaaacaagt tgcttccatt ccgcaggatc ggcacagggc
     6781 cgcgtattag gctatatatt tattgacaat taattaagtg tgtctactta aaattagctt
     6841 gcgctcccct accgggtaag atatgagata cgcaagagtt tggaaaagcg tttggtctgt
     6901 actcaacaca ggaggaaaga caagagggtg tgccgaagtc ggtaatgtga gtggaggcaa
     6961 acagaaaaca gaagaaaagg aaaacgaatg aagaaactat taatcgcatt gatggtgctg
     7021 ggaatggcgg cggtgtcgca ggctgatgtc atcgccaact acacgttcgc gggcagcgcg
     7081 gcgacgagct cggatacgtc ggccctctcg acggcgggat cgttctcgat ctcggccggc
     7141 acgtggacgt acgcgacgtc ctcctcttcc tcgtgggccg gccagggcgg cgagatcccg
     7201 gtggcggatc gcgccacgtg gggcacggcg gcgacatcgt tcgagaccca gaccacgtac
     7261 tggtacttca cggtcgccgt ggacgcgaac taccagctga gcatcacggg gctggacttc
     7321 ctcggtcagc gcaccgtcgc cggcccggcc tcgttcggct gggacgtcaa cggcacgctg
     7381 caggaatcct ggacaccggg cgtgacgacg gtcaacagct acagcagcgc gcaaacggaa
     7441 ggcccgctca cgggcaccgt gacgatcggc atccgcggct ggagcgcgac ctcgacgggt
     7501 aacttccgcg tggacaacgt cgtgctgaac ggcacgatca ccgcgatccc ggagccgggc
     7561 gtgctggcgc tgctgggcct cggcctgggc gcgctgttct acgtccgccg caatcgcaaa
     7621 taagtacact gcgatataag cagaagaacc gccccgattc gtcggggcgg ttcttttttt
     7681 tacgccgacg gcggaccgca tgggcacacc gcccggacag cctcgccttc gcgtccccgc
     7741 cctcaacctc gctcaccggg cgcgatagaa tcccgatgac tcggtcatct cttcctggat
     7801 cgtcaccgcg ggttgccagg tgtccaccac ctggcgattg gaccacgagg cggtttccag
     7861 cgtcaccgtg tgctgcacat cgtaggcttt tcccgggctg atgtcctgca gcataaagat
     7921 aaagaaagtc ccttccgtgc gcgtgtccgc caggcgcgac gccgccttgg ggccttcctc
     7981 ctccagcgag taaaaactgc ggacgtccac cccatgccct ggaaaggccg cctccacctg
     8041 ctcgaccatc ccgataaagg cctcgaagtc cggccaggtc gcgccgatgt ccgcatcgag
     8101 ggcgatccgg gtcccgccgc cgaaatgcgc gacctcccag gcgacgccgt tgctgatgct
     8161 gccgccgagg ttgtcgaacc agaccgcagg tccgcgtaca cggggatatc cgccgcgccg
     8221 tgggcgtcga ggtagccccg gacgtccgcg aaagtgtcgc ggagccccat cagcatttcc
     8281 tttttctggg cggcggtgtt ggccatgggg agaggcctcc tacccgccgc cgggccctgc
     8341 cgcccggatg cgccggagcg tctcccggcc gttcggcagc gacggatcga gctcgagggc
     8401 ccggcgcacg caccgttccg cgtcgggcca ctgttccaac tgggcatgcg cgacgccgag
     8461 gttgaaccag ttcaaggcca cgtcgggccg gccccgggcg gcctgccggt aatgctccag
     8521 cgcggcgtca tactgcttcc gctggaatag aatgttgccg aggttgtagt ggggctccgt
     8581 gtacgtcggg ctccagcgga gggcctcctc gaaggccgcc acggcctcgt ccgggcggtc
     8641 cagccggacc agggtcgtcc cgagcgagtt ccacaccagc accaggtcgg gattcgaatt
     8701 caatacgagc cggaaacagc gcgtcgcctc gtccagcttc ccgaaggaca ccagggcatt
     8761 gcccaggtgg atgagatcgc cgggctccgt cggcgggttg gcgaagacgc gctccaggtg
     8821 ggcccggccc tcttcggggc ggccgagcag ccggagcacg cgcgcgtatt ccgtgtgcgc
     8881 gcgcccgttc tgcggccgct tgtcgatcgt gtcgcgccag aggcacagca ggtcgcggta
     8941 gtcctcgttg cgttcgcgcg ccgccgtccc cagcgtggcc gcccaggcca gcagcacggc
     9001 gatgaacccg ccccgcgcat accgggccgg gaggaggcgc agggcgcgat ccaggaccac
     9061 gacggagagg acggccagca cggccagcgg ggcgtacagc cggtgctcgg cgatcggctg
     9121 cccggcaacc gggatgaagc tggacgaagg cgccagcacc gcgaagaaga agaggccgag
     9181 gtaaccgatg cgcggatgac gagccagggc ccacgccgtg gccagcccca ggcccgccat
     9241 caggcagagc tgcggccaga cgtccgacgc gcgctccacc atctgcgtgc cgtagtagaa
     9301 gatgaggggc gccggccaga ggaacaggcg cagcatgcgg gtgaggcccc aggcctgggt
     9361 cagcaggtaa tgccagtagt ccccgacggg cacggagagg tccggcgcgt actgcgagta
     9421 ggccaggtgc cgtcccagcg gccacaacca ggtgagggcc agcgccaggt agtacagccc
     9481 ccgctgccgg tggaggtctc tccacgacgc gcacaggaaa atgcggtcat acgccaaggc
     9541 cagcagcggc gcggcggcca tggattcctt gctgcccatg cccaggaggc aggccagcgc
     9601 ggagaccgcg aaccagaagc gggcgcgcga agtcgcgggc ccgccggcgg cctcccggac
     9661 ggcccggttc aggccgtaga gcatgaggaa aaggaacagc cccatcgcca cctcgctgcg
     9721 ctgcgagagg taattgacgg cggaggtcag gagcggatgc acgctccaga gcagcgccga
     9781 cgcgaaaccc agccgccgcg cctccgcctc cgtgcgcccg gcggcctcga aggtccgccg
     9841 cgccagtccg aacagcgcga gggtggcgcc caggtgcagc agcaggctgg tccgatgaaa
     9901 gccgcgcgga tccaggccgc cgagggcgta gtccacgcac atggtcaggt tgacgaacgg
     9961 gcgggtgaag aacgtgacgt tcgcgggcgg cgggttcagg gcggcggcca ggggccacca
    10021 ctggcggatg gccgtgttct cgacgatgct caactggtca tccatgagga agggcccgtc
    10081 gagcgcgttg ctgtacgcga gccccgtcgc caggagcagg cccagcgcgc ccagccgcct
    10141 tggaagggtg atcctcacgg cccgcccgcc ggacccgcgc cgcgctggag ctccgcccgg
    10201 attcgctgca gggtcggcca ggccccgggc agcatgggat ccagggccca cgcccgattc
    10261 acgcaccgtt cggcctcgcc gaaattgccg agcgcggccc acgtgacgcc gaggttgaac
    10321 cagttcaggg ccacccccgg cctgtactcg gccgccttct ggtagtgcgc gagggcgtca
    10381 cgggggcggc cgtccttgaa caggagattc gccaggttgt aatgcgcctc ggcataatcg
    10441 ggtcgaagct ccagggcctt ctcaaaagcc gcgacggcct cgtccgtccg ggccatgtcc
    10501 acgagcgtcc tccccatgtt gccgtagctc accgccgact cggggctgag ccggatggcg
    10561 ttccggtagc aggccatggc ctcgtcgaac cgcttgaagt gggcgagcgc cagcccgagg
    10621 ttggccaggt cgcccgcctt ggcgaaggga tcctgcttga tcaattcgag atgggccagg
    10681 ccgttctcaa actgccccgc ctcgcagaga gagcggccgt attcggcgtg ggcgcggagg
    10741 ttgcgggggc gtttctgaac cgtgtcgccc cacaggaccg gttcagacgc gtagaccttg
    10801 ttgcgctcgc ggctggcgat tccgagcgag acgacccagg cgcccagcag ggccgcccgc
    10861 agcgcgcggg gcttcaggtt cacccgggcg gccaggagcg ccgccagcgg ggcgtacatc
    10921 cggtgctcgg ccaccggttg gccggtgatg ggcatgaggc tcgacgacgg cgccagcaca
    10981 ccgaggaaga acaggcccag gaacccgagg cggggatgtc gccagagcgc ccaacccgtg
    11041 accgccagca ggccgatgca cagcacgacg tgcggccaga cgtacacgat ccgatcgaag
    11101 aggccgaccc cgtagtcgaa gatcaggggc gacggccagg ccgcgagccg aaccatccgc
    11161 accaggcccc acgcttgcgt cagcaggtat cgccaataga caaacagcgg gaagcctccc
    11221 tccggcatat ggggcgagta gtgcgcgatg cgccacaccg gccagatcca ggtcagcgca
    11281 agggccaggt agtatcccca gcgccggcgc acaacctcct tccaggatgg gctcaagaag
    11341 atgcggtcat acgccagggc caggaaaggc gcggcggcca tgttctcctt gctgcccatg
    11401 ccgagcaggc aggcgaagac cgacgccgcc agccagcccg cggcccgcgc ggtcccgccc
    11461 gggccggacg cccgctgttc cgccgcccgg ttcagggcgt acaacatgac gaacaggaac
    11521 agcccgacca gcagttctcc gcgctgggag agatacttca cggcggttgt cagcagcgga
    11581 tggaccgtcc agagcagggc cgcggcggcg ccccagccca gcgaatcgcc ccgccccatt
    11641 ttttccgccg tccggcggac cagcccgaag agcgcgagcg tggcggcgat atgcaggagc
    11701 acgttcgttg cccggtaccc ggacggcttg aagccccata tcgcatgatc gacgcagtag
    11761 gaaaggttca gcaccggcct cgtggccagg ttgatcccgc cccctggcgc cggattgagc
    11821 gccgcgctca agggccatgg ccgccaggcg gaaggattct cgacgaagtt ggtcgtgtca
    11881 tcgaggacga acgggccgtg caggccgttg ccctgggcaa ggacggccgc cacggccagc
    11941 cccaggccga tccaccagga ccgcgccgat gcgcgggaac tgcctttggc ttccgtttca
    12001 cccatgctgc gcccgccatg ctctcctgcc atctatgaca gacgagacgc gaccgatcaa
    12061 ggttgcagga gcggcggcca gccctctacg cgcgcgccag cccggtgtac gatgcccttc
    12121 agcagagggc gcatgccatg atcgagtcca tcgcggggcg cgaacggcgg cggcatcggc
    12181 ggctggcctg cgagcggccg gcctccatcc acgtggtcca ctgcccggac gatccccggc
    12241 tcgagaggcg cagcctgtac ggcatggtgc ggaacatatc ggcgggcggc ctgaagttca
    12301 cctgcccgca atcccttccg cccggcacgc tggtgcactt ggatgtcgcc atgccggaac
    12361 gcggctgtct cttcaagctg gccgcgagga ccgtgcgcag cacgccccgc acggaccggt
    12421 tcgaggtggg cgccgcactc gaggattcgt tcttccggcg gcgggatccc tgggcgcgca
    12481 tggtcttcga tgcgctccgg gaactcaacg acccggtgta ggacaagcgg cggaggtcag
    12541 ggaagggatt cggattcgtc ggcgggcgcc gcttcctttt ccacgcgttt gaaggagacg
    12601 ccctccacgc cgacgcccgc gaccgtccat cggtattcgc gcccgccgaa gcggaccgat
    12661 acggtgtcgc ccacgccgac cacctgaccg ttgaccatgg ccacgtgggt ctcgggcccc
    12721 gtcttcataa tcccctgcac ctgcacggtc ttgagggcct cgtcccactg cagcacggcg
    12781 gagaccgttt ccgctccggc cgtcgacgcg gcggcttcgg acgggcgagc gggcggcggg
    12841 ggggcgtagc cgacgggcca gaacggatcc ctccgcgcgg gcgccggttc cggttccgcg
    12901 gcttccgtct cgccggccgg cggcgccatc ggctccgccg cggcgggctc cgaccaaggg
    12961 gaggaagcgg ccgccggggc ctgcccggtc tcgaccatca tggggatgat ctccgattcc
    13021 acgaactcga caaagccggg ttccgggggc gttgcctgtg cgggctccgc ggcacggcac
    13081 accccgacgg ccaggacgag gacccacgcg attcttccgc ccgccttcat catggacagc
    13141 ttgcctcttt ggcctctcga atcacagttc aatttcacgc agcaagatac atgcctttgg
    13201 cgccgggttt caaggcgagc ggccgcggga ccggagccgc gcatcccgat ggccggccgc
    13261 accgggaagg acaggccggc cacggccgat ccgtttcgga aaaagttcag cacaaatgcc
    13321 gcgctttcag gcccatcaag gcgctgtcat ttttgagaaa agaacaaaaa aacatgttgt
    13381 ttttaggcgt tttttaatgc gtgtaagtca acaaataaaa agaggcctcg atttaacggc
    13441 aaatagccct gccccggacc ggaaggtatg aattctgctg ttaagaggta ggcggagtgc
    13501 gcgtcatgcg aaagacagag acacagcgac gggggcggag cggcttcacg ctggtcgagg
    13561 tgatgatcgc ggtcgtgctg gtgctgctgg ccctgggcgg ggcgtatacg ctgatcgtgc
    13621 actcggcgcg ggtcagccgg gcggcccggg atcattacgt ggcggtgaac ctcgcgaaga
    13681 accacctcga gcgcgcgcgc aacttccgct acgcggacct gtacctgctg gcggaagaca
    13741 acctggtgat gaacgagaac ggcggcccgg acaaccgggg gcggttccgg cggacgacgg
    13801 tggtggacga cgactacgcc acgggcgcga cggaaatcac ggtaacagtg caggtccgga
    13861 accgccggac aggcgagttc ggcgaagaga aggagcagat ctcctccctg ttcacggagt
    13921 acctgacgcc ggagatcgaa tgagcacccg ggaatccaga ggcggttttt cgctgacgga
    13981 actgctggtg gccgtcctga tcctcgtgat cacgatggcg ggggccatcg cgggctggct
    14041 gttcgtcgtg cgcggggagc ggatgaactc gatccagcag aagctggaca tggacgtgcg
    14101 aaagtccctg gagcgactga aggcggagat gcgcctctcg accatggacg aggtgtattt
    14161 ctacccggcg ggcgcggggc cttacacggc catcagcttc ccgatggcgc gggacgacga
    14221 cgcggacggc ctggtggagc tggacgagga cgaccacatc atctgggatc agacggtcgt
    14281 gtaccacatc tggcagagca ccccgcacca gttgcggcgg acggtattcg atccccgcaa
    14341 caacagcctg acccccgggc agcggcagga gcagctgaac gcggtggtca cagcgggcag
    14401 cggcgcgggg acgcacaacg gggcgaacgc ctcgacgacc gtcctgttcg agaacctgtt
    14461 cacgtggacc atcctcggca agggggcggt gttcgacgcg taccatccga ccctggcgcg
    14521 gatggtcggc gtgaacctcg ggtccttcct cctctccccg ggcgcgcatg cgtacaagtt
    14581 ctcggtcatc gacaagaacc cgtcgagcac gggatacaag atcgggttgg actcgctgat
    14641 cgtttcgccc agcgggctgg agcgggaggg cgaggtccag acggtgtcgg cgcagtcggg
    14701 cgcgacggcc tcggcggact accgttcggc cggcggctgg agcgggaact atcacctgct
    14761 gttcccggcg acggccgtgg gcaactccat gacgctctcg atggagaacg accggtggga
    14821 ggagacgaac ttccggggca cgggctcgct ctgcgacaac acgatcgtcg tgttcgacga
    14881 cacgctgacg ccgaaggatt tcgtggcgcg gctgcagccg ccgaacacca cctggctggc
    14941 ccaggaccag acgctggacc tgaacagcgg gtcgacgacg aacggcttcc tgcaggggtg
    15001 cgccgtgcgg gtgctggtgc gcggcggcag ccagccggac ggcggggcga tcctgaacag
    15061 cggccccttc aacttcgcgc tgttctacgc cgccgccgag gacccgctgc agatcaccaa
    15121 ggcctatatc gcggaggccg ccagctcctt tggctactcg cccgacatcc tgccggaaac
    15181 cagccgccag ctgtatttcg gcggcagcga gcaggtcaac atctccgaag gctcgtacgc
    15241 cctggggttc ctgggctcgg gccagtcgct cgatatccag aacgaccgga gttacatcgt
    15301 cagcttcctg gtgaccgctg cggagtccga gcgcgccgac gcgcgaacgt ggacggaact
    15361 gcacccggcc accgcgacca acccggcgcc gccgggctgc tacgtcctgc ggggcggcac
    15421 caaccttccg gatgacgtgc tcacccaggc cacgtgggcc ggctatcccg acttcgagac
    15481 gcacgagaag ctctacgccg tggagcgcgt gtacgtcctc gcccccagca acggcacgtt
    15541 cacctcgcag atcgtggaca cccagttgag cgcgccggcc taccagacga tgacctggaa
    15601 ttcggtcaag ccgtccggca cgtcgctggg cctgaagatc cgcgcgggga
//
LOCUS       DAOOTK010000073        15462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2579_length_15462_cov_5.406828, whole genome shotgun sequence.
ACCESSION   DAOOTK010000073 DAOOTK010000000
VERSION     DAOOTK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15462
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_15462_cov_5.406828"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PK634_09035"
     CDS             <1..776
                     /locus_tag="PK634_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97919.1"
                     /translation="LEQNTVLRDGLTLDFAALRILFGAQELARPDALGFRLVLNGELP
                     VERYLYYMAVAGRAPAPPQEGQLAIPATPDVDPAATLADADPWIVAASLFLARKGYGR
                     VSARQVLDRWTSRRDLWDDVCTAQALLFLAGQDAQVVAGARMNREDIEEDLGRLAPAA
                     AGRAEVHVLVFDAVSFQPIAPREVRLEHLEQGPAGAGPAVSGEERVALPESSEGVIPV
                     EPGLYRIRYLDGAVHGTSQAFEAARGRLARVCIMALGGV"
     gene            855..2327
                     /gene="rpoN"
                     /locus_tag="PK634_09040"
     CDS             855..2327
                     /gene="rpoN"
                     /locus_tag="PK634_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOW97920.1"
                     /translation="MFAPSGGGNVVVAMSTGQYMSQTQQQRLQMVLAPQLRQSLALLQ
                     VPILELRTLVQEEMQQNPTLEEKVARDTPVEIEPGTGEPEKDKALDFKEEFEVLARLD
                     DEWREYFHQNQAAHPYTAEDAARRQHFFDSLTQPESLQEHLRNQLIMAELAEEDRRVA
                     EMLVGSINDDGYLVTPLEDLAQGTGFSLDRIKELLQVIQEFDPIGVGARDLKECLMLQ
                     LNRLGKGESTAAAIVRDHLDDLGARRYPQLAKALGITLEEAQEAARFVSTLEPRPGRL
                     FSAESANYVLPEVSVQKSGGEYAIALDRDAVPHLFISRHYRQLMENPDTPADVKEYIR
                     DKIRAGTFLIKSIYQRQQTIYRIAREIVEVQRDFLEHGVSHLKPLTMAEIAAKLGIHE
                     TTVSRAIANKYMQTPRGVYEMKYFFTPGFTTQDGQEVSNKTIKDSIAQFVAKEDPAAP
                     LSDQAMVALLKEKGITVARRTIAKYRDELKILPSHLRRAT"
     gene            complement(2338..2784)
                     /locus_tag="PK634_09045"
     CDS             complement(2338..2784)
                     /locus_tag="PK634_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW97921.1"
                     /translation="MPNITVQTSRILLVEDDHDDAALAQRAFHKANVWNPLDVVRSGQ
                     EAWDYLTHQGAFADAEKFPAPLFVLVDLNLPGMDGRELISRIQADSGLRRIPLLVVST
                     SDYEKDIEFGRKLGVQHYVIKPLQPQNILAFIGALPGFQIILGTAK"
     gene            complement(2795..4315)
                     /locus_tag="PK634_09050"
     CDS             complement(2795..4315)
                     /locus_tag="PK634_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW97922.1"
                     /translation="MSPADPSVARRLLVVEDDETVRRFLVELFRKSGYRADAASNGAE
                     ALELVEENPCDVMILDQKMPGLSGLDVLERLRAIGPPFPVIVLSGHGSEELAVQALHM
                     GATDYVTKAPDPRFGEILLDKTRRAIERFAQEEEQRAHVETLRQRMSELGCLYALEKL
                     FDSVEGSPRVALSAAADILLPGAGGRCCAVIRIGKEEYASANRFESGHVESFDIRERG
                     KTIGTLDIRFRDGAGAPPLSDMERDLMMAVSDRIGHFMDFWHTEQRLRESNAALEEYA
                     YVASHDLQAPLQKIESFAQLLQEDCASRLDESGRYYVSVVHRSAQQMRRLIKDVLALA
                     RLDSDGLAIKPLKLGDILAAVKDQLSQRLVERNAVLATDALPTVPGDETRLTQLFQNL
                     VGNALKFNDKPEPRVEVGAVEEPGHWRIYVRDNGIGMTREDAGRLFVPFKRLHTGEKY
                     EGTGIGLALCRKIVRQHGGTIDVDAEPGKGCTFWIRLPKEPATAAPNDVNTSAQPP"
     gene            complement(4324..5736)
                     /locus_tag="PK634_09055"
     CDS             complement(4324..5736)
                     /locus_tag="PK634_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOW97923.1"
                     /translation="MAGVLLAAAGSSARLIPIIVPTQGEDLRNAPYLEKTAAYLSQRL
                     PGDEFVVSRAGANWINQVDKTGPGLLFLDPAQFVQLESRVSLRPVAAVERFVQGNRTR
                     HFGGAVFTLRNSAVQRPEDLKNVAVAIVDGDPLGATLSVLRELKARGPGLQETFGRLI
                     TNPNDQAVVEIVMAGQAEAGVVTAGVLEQMAQVGRLNLADIRVLALSAEPTPAGFPFL
                     ASTRLYPERLLAALPGTDSDLVDQIAALLLTAGPADLDPGGTMNAGWTAPDARETVRQ
                     LLRDLHHPPYEEYGRLTFGAVLRQYMYWFIGAGVLFVVLALLLSYVTSLNRALGEEVE
                     ERKEAQRSLESSIERFEHIASCSGDWIWETDLEERFTYSNNALKKLLGWPPKELLGKP
                     LFEILSSAEKEKAGERKRVLAGDTATIFHRRLTLLTSDGRVAVHECTAGPIRDKRGEI
                     TGYRGINRDVTAEARMVSFR"
     gene            6025..9294
                     /gene="mfd"
                     /locus_tag="PK634_09060"
     CDS             6025..9294
                     /gene="mfd"
                     /locus_tag="PK634_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOW97924.1"
                     /translation="MSDALGPWVLGEEDRRHLADIFRSGGPRSVQDLPVSAQAFLSLA
                     LRGAERRPVVWVGDSARTLDQFHQDALVLSGDRKEGLACFPAREEGAGRSAHLDRLGD
                     RLQTLRACLQPGRPLLVATCIQALFQDTPAPAALERARRRLSVGDSADPEELAAGLER
                     QGYAFEVEVLQKGQAARRGGILDLWPPTEDWPLRLEFFGSTLESIRTFDPAGQRSLEK
                     KGALDLAPAGESLESSEAAADLLAYLPPETLWIWNEPQSIFHHAEMYRAMLPENSPSR
                     AAADVERLRLRVQQRFRGGQLVIGPDVVETAPPRELGLRPCAGLPGLEGEGAFRPDLL
                     DEARRRFLDELCGRARKGERIVFFFDTEGARERFLELHGKKTGFEVRLGALSEGFEAP
                     PSKLVVVAESDLYGFRKRLPGRYELHGRRRGARADAGARLAEWMDIQPGELVVHVDHG
                     IGKYLGLYEIEFDGRQQEVMAVEYADQAKLYVPVSQAHLLSRYVGLGRRRPDLHALGG
                     KRWTREKVAAEGAVRDLAASLIETQARRDLLEGHAFPPDTPWQHEFEAAFPFRETPDQ
                     HRAILDVKKDLQSRRPMDRLVCGDVGYGKTEVAMRAAFKCVQDGRQVAVLVPTTILAQ
                     QHFDTFGERLAAYPVTTEMLSRFRSRAAQHDTARRLAEGAVDIVIGTHRLLQPDVRFK
                     DLGLVIIDEEQRFGVAHKEHFKRLRELVDVLTLTATPIPRTLYLSLTGARDMSTIETP
                     PQDRLPIETLVAQDSDELVRAAILRELNRGGQVFYLHNRVTTIELVRRRLQERVPEAR
                     VEVGHGQMHEKELAEVMHRFVRGEFDVLLCTTIIESGVDIPNVNTILIDRADRFGLAD
                     LYQLRGRVGRYKHQAYAYFLLPRHGRLFDNARRRIGAIRRYSGLGAGFKLALRDLEIR
                     GAGNLLGAEQSGHITAVGFDLYCQLLKRTVARLKGEPAPPIIDVMLKLYFLDLSLAPE
                     CEDRSAVFPAAYIEDENLRLGLYRKLASAGTEPELDALAVELRDRFGPLPAPAARLLE
                     LARLRIRAAGRGIGSIEVREDKVMMMQGAAYIMSNNRFPRLKSPEPDRRLAELLVLVA
                     G"
     gene            9337..11313
                     /locus_tag="PK634_09065"
     CDS             9337..11313
                     /locus_tag="PK634_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97925.1"
                     /translation="MKTCVRIIVPGLLLAASSALARPAAVDLGRWQTPIRNQGGRGNC
                     FIHGTVAAMEAAYKRAGSGDLDLSELFSDYVGQVFFLETVRMDGHWYTSDMRVPGATE
                     RETSIQSDHAMSVESASPCMTLAIPEDRCMPYRYGPADLGEHADKNDPFWANQLTVSA
                     FNLDPSNLPYSALSAPRYYRIRSIEWLPKDDARNPAAIEAVLAGGREVIWDFRMAGDI
                     SGKVWQYNGPAGDAYPHRMLIIGYNRTDARHPYFLVKNSWADVGVYDTRDCITRISYD
                     YLQYGEWASYIKEIEPPRPWPELQFIGRWSLEMGPRRGLLDIYHLPGMMRGSFEHNQF
                     RDEQGRPLEDRRLGTFYEKGDPNDAYRVNGIVRGHSIELYIDFSHPAARWDRLQGWKV
                     SFTLNPRDPHRLIGRYDARDGRRGEARAERNRAPISPLGPRPPEEESQALLARDEMEK
                     RKRWDAEAVERAELELKKQAEEALKIAENERLKKEANAPAADSPILLKWRELGGADGF
                     LGGAQTEERACPDGRGHYIHFAGGSIYWTPETGAHAIYGAIREKWASLGWETCAFLGY
                     PITDETGTPDGVGRFNHFEKGGSIYWTPETGAFEVHGPIRERWQALGWETGRLGYPVS
                     DVEMADGKEGLVTRFQRGLIRWHPARGVTVETEK"
     gene            complement(11485..15426)
                     /locus_tag="PK634_09070"
     CDS             complement(11485..15426)
                     /locus_tag="PK634_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOW97926.1"
                     /translation="MLAYVVHPQHPGPVYFASMTGSALGALAPAVLLGAIGEPALLAA
                     DILPYPSQPRSPKSKPRPAFAEANDRYASAEAWPADAATIEGIHDMQGYRFVSVRVNP
                     LFYVGADRALHLRPAIDVTVSYDAPATPRTISAKQKAQFEPLVNSLVVNPSSSDEFAP
                     ASRTETRAALDYLIITSPALSNAFQQLATYRASSAGGSYTTTVLTTNYISSTFTGADI
                     QQKIRACISNRVATMGTTMVVLGGDDTIVPDRDCYVAVVSGDTYETNMPTDLYYSGLG
                     GNWNSDSDAKYGETSDNVDMAWDVIVARIPMRTAAQVTNYVNKVIAYDADSSRLADKI
                     VLGGPEAWSTYSGTARPSDDVTIDGHAGFRSTSPAHTYVSDSEMWLRRLYRDGIYPYW
                     PGAVSIICDTITSWDSSTCGDHSESAANTLAAFNKNYTHLMFSGHGAPQEWGLESGSF
                     DNTDAASMTGMAAFVYTDACLTGHFDRDSNNIDGYGYTTEPCLGEGFLRNYRNAGGAV
                     AYMGCARYGWGEPDETPASNTSDGGPSTIYAYKFYKRFHETANRTVGTAFAMHKADMI
                     SSCGTDEAERWIQFGLNLLGDPALKLPVETVPTNTPPVLASIGNKSATWSNTVQFAVT
                     AAATDGDAVTLTISNKPAAATFWSSGTNGLFSWTNAGPVGVYTMSVYAADNDGADFET
                     FTITVGAGGSAADLFISEYIEGSSNNKAVEIYNGTGASVDLATGNYDLRLYFNGSTSY
                     STIDLTGTLAAGDVFVIANSGANAAILAQADLTSGSVTFNGNDVVALAKNSANMDVLG
                     TIGLNVTNMADVTKVRKSSVSQGTTTYTASEWDDYAADTTSYLGSHTFSGGGSTPTAA
                     EITITDPASASTSVAYATSTYDVSGTCNTAAVGQIRWTNSLTGASGNTAAFTNWTIGG
                     VALNVGNNSIFVSVTNASGTVASDTVIINRAALVATNKPTFSYVPSGTNQSVTVSNAL
                     SVTVTATDEDGETVSLVPSSLPAGSAFTGGTNAASVSSAFTWTPTVTGTYSAAFKAED
                     NDGAVTSRIQIVVTESSAGGGTETFANFSEGGTYASGTFAGQDGSTWTYSFSRGDLSI
                     NGSSPTLKKAAGTYVRSGTLSGGVGALTLKYRKPYTTALSCDIFVNDTLVGTISGGDG
                     TTQTWSSSSINISGDVVLLFTNKTSTSGQITLDDIQWTGYGSDAPAYYTDWAAGYDLD
                     PEGPNGGMDDDYDNDGVPNIDELVGGSHPTNGASMFEIVDQDKQAAANQFTLTIPAVT
                     GRVYSVYYKNTMTNADWTLERAVTNIAAGSQNLYVTNSLDARFYRVSARMP"
BASE COUNT         2413 a         5138 c         5376 g         2535 t
ORIGIN      
        1 ttcttgagca gaacaccgtt ctgcgggatg gcctcaccct tgatttcgcc gcgctccgga
       61 ttctcttcgg cgcgcaggag ttggccaggc ctgacgcgct gggtttcagg ctcgtcctga
      121 acggggaact gccggtggag cgctacctgt actacatggc cgtcgccggc cgcgcgccgg
      181 ccccgccgca ggagggacag ctggcgatcc ccgcgacgcc cgacgtggat ccagccgcca
      241 cgctggcgga cgccgatccc tggatcgtgg cggcgtcgct gttcctcgcg cgcaaggggt
      301 acggccgggt atcggcccgg caggtcctgg atcggtggac ctcgcggcgg gatctttggg
      361 atgatgtctg cacggcccag gcgctgttgt tcctggccgg gcaggacgcc caggtcgtgg
      421 cgggcgcgcg gatgaataga gaggacatcg aggaggacct cggccggctg gcgccggcgg
      481 cggccgggcg ggccgaggtg cacgtgctgg tcttcgatgc cgtctcgttc cagccgatcg
      541 cgccgcgcga agtccgcctc gaacatctgg agcaggggcc ggccggcgcg ggtccggcag
      601 tgagcgggga ggagcgggtg gccctgccgg aatcctcgga gggagtcatt ccggtcgagc
      661 ccggcctcta ccgcatccgg tacctggacg gcgccgtgca cggcaccagc caggccttcg
      721 aggctgctcg cggccggctg gcgcgggtct gcatcatggc cctcggcggg gtctgacgga
      781 tccggcgcgc cggatgccgg ccccgggacc gcgctcgcat tcctgcatcg ccgccgttcc
      841 tgcctgaaaa gaccttgttc gccccctctg gcggcggtaa cgtggtcgtc gccatgagca
      901 ccggccagta catgtcgcag acgcagcagc agcggctgca gatggttctg gcgccccagt
      961 tgcgccagtc cctggccctg ctccaggtgc ccatcctcga gctgcggacg ctggtccagg
     1021 aggagatgca gcagaatccc accctcgagg aaaaggtggc ccgggacacg ccggtggaga
     1081 tcgagccggg aacgggcgag ccggagaagg acaaggcgct ggatttcaag gaggaattcg
     1141 aggtcctggc ccggttggac gacgagtggc gggaatactt tcaccagaac caggccgcgc
     1201 atccctacac ggccgaggac gcggcgcggc ggcagcactt cttcgattcg ctgacccagc
     1261 ccgagtcgct ccaggagcac ctgcgcaacc agctgatcat ggccgaactg gccgaggagg
     1321 accggcgcgt cgccgagatg ctggtcggca gcatcaacga cgacggctac ctcgtcacgc
     1381 ccctcgagga cctggcccag ggcaccggct tcagcctcga ccggatcaag gagctcctgc
     1441 aggtcatcca ggaattcgac ccgatcgggg tgggggcgcg cgacctcaag gaatgcctga
     1501 tgctgcagct gaaccggctc ggcaagggcg aatccacggc ggcggccatc gtccgcgatc
     1561 acctggacga cctgggcgcg cggcgctatc cgcaactggc caaggccctg gggatcacgc
     1621 tggaggaggc gcaggaggcc gcccgcttcg tctccacgct ggagccccgg cccggccgcc
     1681 tgttcagcgc cgaatccgcg aactacgtgc tgcccgaggt gtcggtccag aaatcgggcg
     1741 gcgaatacgc catcgccctg gatcgcgacg ccgtgccgca cctgttcatc agccggcatt
     1801 accggcagct gatggagaac ccggacacgc cggccgacgt caaggagtac atccgcgaca
     1861 agattcgcgc cgggacgttc ctgatcaaga gcatctacca gcggcagcag accatttacc
     1921 ggatcgcccg cgagatcgtc gaggtccagc gggacttcct ggagcacggc gtgtcgcacc
     1981 tgaagcccct gacgatggcg gagatcgcgg cgaagctcgg catccacgag accacggtca
     2041 gccgggcgat cgccaacaag tacatgcaga cgccgcgggg cgtctacgag atgaaatact
     2101 tcttcacccc cggcttcacg acgcaggacg ggcaggaggt ctcgaacaag accatcaagg
     2161 actccatcgc ccagttcgtg gccaaggaag acccggccgc gccgctctcc gaccaggcca
     2221 tggtggcgct gctcaaggaa aaggggatca ccgtggcgcg ccggacgatc gccaagtacc
     2281 gggacgagct gaagatcctg ccgtcccacc tgcggcgcgc cacctgaacc gcgggcccta
     2341 cttcgcggtg ccgaggatga tctggaaccc cggcagcgcc ccgatgaacg ccaggatgtt
     2401 ctgcggctgc aggggcttga tcacgtagtg ctgcacgccg agcttgcggc cgaactcgat
     2461 gtccttctcg tagtccgacg tgctgaccac gagcagcggg atccggcgga ggccggagtc
     2521 cgcctggatc cggctgatca gctcgcggcc gtccatgccc gggaggttga ggtccacgag
     2581 cacgaagagc ggcgcgggaa acttctccgc gtccgcgaac gcgccctggt gcgtcaggta
     2641 atcccaggcc tcctggccgg accggaccac gtcgagcggg ttccagacgt tggccttgtg
     2701 aaaggcgcgc tgggccagcg cggcgtcatc gtgatcatcc tcgaccagca ggatacgaga
     2761 ggtctgaacc gttatgttgg gcatgggctc ctcctcatgg gggttgcgcg gatgtgttca
     2821 catcgttcgg ggcggcggtc gcgggttcct tggggagccg gatccagaac gtgcagcctt
     2881 ttcccggctc cgcatcgacg tcaatcgtcc cgccgtgctg ccgcacgatc ttccggcaga
     2941 gcgccaggcc gatgccggtg ccctcgtatt tctccccggt gtgcagccgc ttgaacggca
     3001 cgaagaggcg gccggcgtcc tcgcgggtca tcccgatccc gttgtcgcgc acgtagatcc
     3061 tccagtggcc cggctcctcc acggcgccca cttcgacgcg gggctcaggc ttgtcgttga
     3121 acttcagcgc gttgccgacc aggttctgga acagctgcgt cagccgcgtc tcgtcgcccg
     3181 ggacggtggg cagggcgtcc gtcgccagca cggcgttccg ctcgaccagg cgctgggaaa
     3241 gctggtcctt gaccgcggcg agaatatcgc cgagcttcag gggcttgatc gcgaggccgt
     3301 ccgaatccag ccgcgcgagc gccagcacgt ccttgatcag gcgccgcatc tgctgggcgg
     3361 accggtgcac gaccgagacg tagtagcggc ccgactcgtc cagccgggag gcgcagtcct
     3421 cctgcagcag ctgcgcgaag ctttcgatct tctgaagggg cgcctgcaga tcgtgcgagg
     3481 ccacgtaggc gtattcctcc agcgcggcgt tggattcgcg caggcgctgc tccgtgtgcc
     3541 agaagtccat gaaatgcccg atccggtcgc tcacggccat catcaggtcc cgctccatat
     3601 cggagagcgg gggcgcgccc gccccgtcgc ggaagcggat gtccagcgtc ccgatcgtct
     3661 tcccgcgctc gcggatgtcg aaggattcga cgtggccgga ttcgaaccgg ttggcggagg
     3721 cgtactcctc cttgccgatc cggatcacgg cgcagcagcg cccgcccgcg ccggggagca
     3781 ggatgtccgc ggcggcgctc aaggccacgc gcggcgaacc ttccacgctg tcaaaaagct
     3841 tctccagggc atacaggcag ccgagctcgg acatgcgctg gcggagggtc tcgacgtggg
     3901 cgcgctgctc ctcctcctgc gcgaagcgct cgatcgcccg gcgcgtcttg tccagcagga
     3961 tctcgccgaa acgcggatcg ggggccttcg tcacgtagtc cgtcgcgccc atgtgcaggg
     4021 cctgcacggc cagttcctcg ctgccgtggc cggaaaggac gatgacggga aacggtgggc
     4081 cgatggcgcg caggcgctcc aggacgtcga gcccggacag gcccggcatc ttctggtcca
     4141 ggatcatcac gtcgcacggg ttctcctcga cgagctcgag cgcctcggcg ccgttcgacg
     4201 cggcatccgc ccggtagccg ctcttccgga agagctccac gaggaaacgg cggacggtct
     4261 cgtcgtcctc caccacgagc aggcgccgcg caacgctggg atcagccggg ctcatgtcgt
     4321 tgttcagcgg aagcttacca tgcgggcctc ggccgtcacg tcccggttga tgccgcgata
     4381 gcccgtgatc tcgccccgct tgtcccggat cggcccggcc gtgcattcgt gaacggccac
     4441 ccgtccgtcc gacgtcagca gggtgagccg gcggtggaat atcgtcgccg tgtccccggc
     4501 cagcacccgc ttgcgctcgc cggccttctc cttttccgcg ctgctcaaga tctcgaacag
     4561 cggcttgccg agcagttcct tcggcggcca gcccagcagc ttcttgaggg cgttgttgga
     4621 ataggtgaag cgctcctcca ggtccgtttc ccagatccag tcgcccgagc aggacgcgat
     4681 gtgctcgaac cgctcgatgc tggactccaa ggagcgctgg gcctccttgc gctcctcgac
     4741 ttcctcgccg agcgcccggt tgagcgaggt gacgtaggag aggagcagcg ccagcacgac
     4801 gaacaggact cccgccccga tgaaccagta catgtactgg cgaaggacgg cgccgaacgt
     4861 cagccgcccg tactcctcgt acggagggtg gtgcaggtcc cggaggagct ggcgcacggt
     4921 ttcgcgggca tcgggcgcgg tccagcccgc gttcatggtt cccccgggat ccaggtccgc
     4981 gggccccgcc gtcaggagca gcgcggcgat ctggtcgacc aggtccgaat cggtgccggg
     5041 cagggcggcc aggaggcgct ccgggtagag acgggtgctg gcgaggaacg ggaacccggc
     5101 cggcgtgggc tccgcggaca gcgcgagcac ccggatgtcg gctaggttca gccggccgac
     5161 ctgggccatc tgttcgagaa cgccggccgt cacgactccg gcttccgcct ggcccgccat
     5221 gacgatctcc acgaccgcct ggtcgttggg attcgtgatc agccggccga aggtttcctg
     5281 gaggccgggc ccgcgggcct tcaattcgcg cagcaccgac aacgtggcgc cgagcggatc
     5341 gccgtccacg atcgcgacgg cgacgttttt caaatcctcc ggacgctgca cggcgctgtt
     5401 acgcagcgtg aacaccgcgc cgccgaaatg gcgggtgcga ttcccctgca caaatcgttc
     5461 caccgccgcc acggggcgca acgatacgcg ggattccagc tgaacgaact gcgccggatc
     5521 caggaagagc aggcccggcc cggtcttgtc cacctggttg atccagtttg ccccggcgcg
     5581 actcacgacg aactcgtccc ccggtagccg ctgcgacagg taggccgccg tcttttccag
     5641 gtacggggcg ttgcgcaggt cctcgccttg ggtgggcacg atgatcggta tgagccgggc
     5701 cgaagagccg gcggctgcca gcagcacgcc cgccacgaag gcccttcgcg cagaacattt
     5761 gcatcgccat ccgcaggttg tcttgtgcat gagacctccc gtcatccact tgacttctcc
     5821 ccgcttcgct ttctttttag caccgataac cgctcgcagg ccaatacata atgaccacgt
     5881 aggggtaatc tgacggaccg cgccgtcgtt ctcgtgaaat gcgtaacgcg gcttcggccg
     5941 cgatccgctc tcccgcgcgc gagccgcatc ccggcatgcg cccgccattg accatgccgg
     6001 acgcttccgg tagcgtgaag gcggatgagt gacgccctcg gtccgtgggt gctgggcgaa
     6061 gaggatcggc gacacctggc ggatattttc cggtcgggag ggccgcgctc tgtccaggac
     6121 ctgccggttt ccgcgcaggc tttcctttcg ctggcgttgc gcggggccga acggcggccc
     6181 gtggtctggg tgggcgacag cgcccggacc ctggatcaat tccaccagga cgcgctggtc
     6241 ctctccggcg accgcaagga aggcctggcc tgtttcccgg cccgcgagga gggcgccggc
     6301 cggtctgcgc atctcgatcg cctgggtgat cgcctgcaga cgctgcgggc ctgtctccag
     6361 cccggccgcc cgctcctggt cgccacctgc atccaggccc tgttccagga tacgcccgcg
     6421 cccgccgcgc tggaacgggc ccgccggcgg ctgtccgtcg gcgattcggc ggaccccgag
     6481 gaactggcgg ccggcctcga acggcagggt tatgctttcg aagtcgaggt cctgcagaaa
     6541 gggcaagccg ctcgccgcgg gggcatcctc gacctgtggc cgccgaccga ggactggccc
     6601 ttgcgcctgg agttcttcgg cagcacgctg gaatccattc gcacgttcga tcccgccggg
     6661 cagcgctcgc tggagaaaaa gggcgccctg gatctcgcgc ccgccgggga atccctcgaa
     6721 tcgtcggagg ccgccgccga tctcctggct tacttgccgc cggagacgct ttggatatgg
     6781 aacgaaccgc agagcatctt ccatcatgcc gagatgtacc gcgcgatgct tcccgagaac
     6841 tcaccatccc gcgcggcggc ggatgtcgag cggctccgcc tccgggtcca gcagcggttc
     6901 cggggcggcc agctggtcat cgggccggac gtcgtcgaga ccgcgccgcc ccgggaactg
     6961 ggcctgcggc cctgcgccgg cctgcccggc ctcgagggcg agggggcctt ccggccggac
     7021 ctgctggacg aggcgcgacg ccggttcctg gacgagttgt gcggccgagc tcggaagggc
     7081 gagcggatcg ttttcttctt cgacacggaa ggcgcgcgcg agcggttcct ggaactgcac
     7141 gggaagaaga ccgggttcga ggtccggctg ggcgcgctgt cggagggatt cgaggcgccg
     7201 ccctcgaaac tggtcgtggt ggccgagtcg gacctgtacg gtttccgcaa gcggctgccc
     7261 ggccggtacg aattgcacgg gcggcggcgc ggcgcgcggg cggacgcggg cgcgcggctg
     7321 gcggaatgga tggacatcca gcccggcgag ctggtcgtgc acgtggacca tggcatcggg
     7381 aaatacctcg gcctctacga aatcgagttc gacggccggc agcaggaggt catggcggtc
     7441 gagtacgccg accaggccaa gctgtatgtg ccggtctcgc aggcgcacct cttgagccgc
     7501 tacgtcgggc tcgggcgccg ccggcccgac ctgcacgccc tgggcggcaa acgctggacg
     7561 cgcgagaagg tcgcggcgga gggcgccgtg cgcgacctgg ccgcctcgct gatcgagacc
     7621 caggcccggc gcgatctgct cgagggccac gccttcccgc ccgatacgcc gtggcagcac
     7681 gagttcgagg ccgcctttcc cttccgcgag acgccggacc agcaccgcgc gatcctggac
     7741 gtgaagaagg acctgcagag ccgccgcccc atggaccggc tcgtgtgcgg cgacgtgggt
     7801 tacggcaaga ccgaggtggc gatgcgcgcc gccttcaagt gcgtccagga cggccggcag
     7861 gtcgccgtgc tcgtgccgac caccatcctc gcccagcagc acttcgacac gttcggcgag
     7921 cgcctcgcgg cgtacccggt gaccaccgag atgttgagcc gcttccgatc ccgcgccgcg
     7981 cagcacgaca ccgcgcggcg gctggcggag ggcgcggtgg acatcgtgat cggcacgcac
     8041 cgcctgctcc agccggacgt ccggttcaag gatctcgggc tcgtgatcat cgacgaggag
     8101 cagcgcttcg gcgtggccca caaggagcat ttcaagcggc tgcgcgaact ggtggacgtc
     8161 ctcacgctga cggcgacgcc gatcccgcgc acgctctact tgagcctgac cggcgcccgc
     8221 gacatgagca cgatcgagac cccgccccag gaccggctgc ccatcgagac cctcgtcgcc
     8281 caggactccg acgagctggt tcgcgccgcg atcctgcgcg agctcaaccg cggcgggcag
     8341 gtgttctatc tccacaaccg cgtcacgacg atcgagctcg tgcgccggag gctgcaggag
     8401 cgggtgcccg aggcgcgcgt cgaggtgggc catgggcaga tgcacgagaa ggagctggcc
     8461 gaggtcatgc accgcttcgt gcgcggcgag ttcgacgtgc tgctctgcac gacgatcatc
     8521 gagagcgggg tggacatccc gaacgtcaac accatcctca tcgaccgcgc cgaccggttc
     8581 ggcctggccg acctctacca gctgcgcggc cgggtggggc ggtacaagca ccaggcctac
     8641 gcgtacttcc tcctgccgcg ccacgggcgg ctgtttgata acgcgcggcg ccgcatcggc
     8701 gcgatccggc gctactcggg gctcggcgcg gggttcaagc tcgcgcttcg cgacctcgag
     8761 atccgcggcg cgggcaacct gctcggcgcc gagcagagcg ggcacatcac cgccgtcggg
     8821 ttcgaccttt actgccagct cctcaagcgc accgtcgcgc ggctcaaggg cgagcccgcg
     8881 ccgccgatca tcgacgtcat gctcaagctg tatttcctgg acctgtccct ggccccggag
     8941 tgcgaggacc ggtcggccgt gttcccggcc gcctacatcg aggacgagaa cctccggctc
     9001 gggctctacc gcaagctggc ctccgcggga accgagcccg agttggacgc gctggccgtg
     9061 gaactgcgcg accggttcgg cccgctgccc gcgcccgccg cgcggctact ggagctggcc
     9121 cggctgcgca tccgggccgc cggccgcggc atcgggtcca tcgaggtccg cgaggacaag
     9181 gtcatgatga tgcagggcgc ggcgtacatc atgtccaaca accgcttccc gcgcctgaaa
     9241 agccccgagc ccgaccggcg gctggcggag ctgctggtcc tggttgcggg ttgatgcgca
     9301 gttggctatg aatgcacgag aggagcgctc ttcccgatga aaacctgcgt gcggataatt
     9361 gtcccgggcc tgcttcttgc ggcatcctcc gcgctggccc ggccggcggc ggtggacctg
     9421 ggccggtggc agacgccgat ccggaaccag ggcggtcggg gaaactgctt tattcacggc
     9481 accgtcgcgg ccatggaggc ggcctacaag cgcgcgggct ccggcgacct cgatctgtcc
     9541 gagctgttct ccgattacgt gggccaggtt ttcttcctgg agaccgtccg gatggacggg
     9601 cactggtaca cctccgatat gcgcgtgccc ggcgcgaccg aacgcgagac gagcatccag
     9661 tccgaccacg ccatgtcggt cgagtccgcc tcgccgtgca tgacgctcgc catccccgag
     9721 gaccggtgca tgccgtaccg gtatggcccg gccgacctgg gcgagcacgc cgacaagaac
     9781 gatccgttct gggccaacca gctcacggtc agcgccttca accttgatcc gtcgaacctg
     9841 ccgtactcgg ccctgtccgc cccgcgctac taccggatcc gctcgatcga gtggctgccg
     9901 aaggacgatg cgcggaatcc cgccgccatc gaggcggtcc tcgccggcgg ccgcgaggtg
     9961 atctgggatt tccgcatggc gggggatatt tccgggaagg tctggcagta caacggcccg
    10021 gccggcgacg cgtatcccca ccggatgctg atcatcggct acaaccggac cgacgcgcgg
    10081 catccctact tcctcgtgaa aaacagctgg gcggacgtgg gcgtgtacga cacccgcgat
    10141 tgcatcacgc gcatcagcta cgactacctc cagtacggag agtgggcctc gtacatcaag
    10201 gagatcgagc cgccgcggcc gtggcccgaa ctccagttta tcggccgctg gagcctggag
    10261 atgggtccgc gccgcggcct tctcgatatc taccacctgc cgggcatgat gagaggttcc
    10321 tttgagcata accagttccg ggatgaacag ggacgtccgc tggaggaccg gcgcctcggc
    10381 acgttctacg aaaagggcga tccgaacgac gcgtaccgcg tcaacggcat cgtccggggc
    10441 cacagcattg agctgtacat cgattttagc catccggcgg cccggtggga ccggttgcag
    10501 ggttggaagg tcagcttcac cctgaacccg cgggatccgc accggttgat tggccgctac
    10561 gacgcccgcg acgggcgccg gggcgaggcg agggcggagc gcaaccgggc gccgatctca
    10621 cccctgggcc cgcgcccgcc ggaggaggaa agccaagcgc tcctcgcgcg ggacgagatg
    10681 gaaaagcgga agcgctggga cgcggaagcc gtcgagcgcg ccgagctgga attgaagaag
    10741 caggcggagg aggcgctcaa gatcgccgag aacgagcggc tgaagaagga ggcgaacgcg
    10801 ccggccgcgg attcgccgat ccttctgaaa tggcgcgaac tgggcggcgc ggacggcttc
    10861 ctgggcggcg cgcagacgga agaacgggcc tgcccagacg gccgcggaca ctacatccat
    10921 ttcgccggcg gctcgatcta ctggacgccg gagaccggcg cccacgccat ctacggggcc
    10981 atccgcgaaa aatgggcatc gctcggctgg gagacctgcg cgttcctggg ctatcccatc
    11041 acggacgaaa cgggaacgcc ggacggcgtc ggccggttca accacttcga gaagggcggc
    11101 tcgatctact ggacgccgga gaccggcgcg tttgaggtcc atggcccgat ccgcgagcgg
    11161 tggcaggccc tgggctggga gacgggacgg ctgggctacc ccgtctccga cgtcgaaatg
    11221 gccgacggca aggaaggcct cgtcacccgc ttccagcgcg gcctgatacg ctggcatccc
    11281 gcgcgcggcg tgacggtgga aacggagaag taacgccgtt tactgaatga aactgcacga
    11341 tgcgtcttgc tgtgggtagg gcgcgctcgg cgagcgcgcc gcggcggctt cagcgaagcc
    11401 gccctacctc acgaatcaat gaccatggcg ccgtttcaaa acgaagagga cctgaaacgg
    11461 ccctgcctgc gccacccgtc ctgactacgg cattcttgcg ctgacccggt agaaccgcgc
    11521 gtcgaggctg ttggtcacgt acaggttctg cgaacccgcc gcgatgttgg tcaccgcgcg
    11581 ttccagggtc cagtccgcgt tggtcatcgt gttcttgtag tagacgctgt acacgcggcc
    11641 cgtcaccgcc gggatcgtca gggtgaactg gttggccgcg gcctgcttgt cctgatccac
    11701 gatctcgaac atggaggcgc cgttcgtcgg gtggctgccg ccgacgagct cgtcgatgtt
    11761 cgggacgccg tcgttgtcat aatcgtcgtc catgccgccg ttggggccct cggggtccag
    11821 gtcgtagccc gccgcccagt cggtgtagta ggcgggggca tcgctgccgt agcccgtcca
    11881 ctggatgtcg tccagcgtaa tctggccgct ggtggaggtc ttgttggtga acagcagcac
    11941 gacgtcgccg gagatgttga tcgaggaact gctccacgtc tgggtggtcc cgtcgccgcc
    12001 ggagatggtg ccgaccagcg tgtcgttaac gaagatatcg cagctcaagg ccgtggtgta
    12061 gggcttgcgg tacttgagcg tcagggcgcc cacgccgccg gagagggtgc ccgagcggac
    12121 gtaggtgccg gcagcttttt tgagggtggg cgaactgccg ttgatgctca agtcgccgcg
    12181 cgagaagctg taggtccagg tcgatccgtc ctggccggcg aacgtgccgc tggcgtatgt
    12241 gccgccctcc gagaagttgg cgaaggtctc cgttccgccg cccgcggagg attcggtcac
    12301 cacgatctgg atccgcgagg tcacggcgcc gtcgttgtct tccgccttga acgcggccga
    12361 gtaagtgccc gtgacggtcg gcgtccaggt gaacgccgac gaaacggacg ccgcgttcgt
    12421 gccgcccgtg aacgcggagc ccgcgggcag gctggacggc acgagggaga cggtctcgcc
    12481 gtcttcgtcg gtggcggtca ccgtgaccga cagggcgttg ctcaccgtga cggactggtt
    12541 cgtccccgag ggcacatagc tgaaggtagg cttgttcgtg gcgaccagcg cggcccggtt
    12601 gatgatcacg gtgtcgctag cgaccgtgcc ggaggcgttc gtgacgctga cgaagatcga
    12661 gttgttgccc acgttcaggg ccacgccgcc gatggtccag ttcgtgaagg ccgcggtgtt
    12721 gccggaggcc ccggtcagcg agttggtcca ccggatctgg ccgacggcgg ccgtattgca
    12781 cgtgccgctg acgtcatagg tgctggtcgc gtaggcgacc gaggtggagg ccgaggccgg
    12841 gtcggtgatg gtgatctcgg ccgccgtcgg cgtcgagccg ccgccgctga acgtgtggga
    12901 gccgagatag cttgtcgtat ccgcggcata atcatcccac tccgaggcgg tgtaggtggt
    12961 cgtgccctga ctcacggaac tcttgcgtac cttggtcaca tcggccatgt tggtgacgtt
    13021 cagcccgatc gtgcccagga cgtccatgtt ggccgaattc ttggcgaggg ccacgacgtc
    13081 gttcccgttg aaggtcacgc tgccggaggt gaggtcggcc tgcgccagga tggcggcgtt
    13141 ggcgccggaa ttggcgatga cgaacacgtc ccctgcggcg agggttccgg tcagatcaat
    13201 ggtcgaataa gacgtggagc cgttgaagta caggcgcagg tcataattgc ccgtcgccag
    13261 gtctaccgag gcccctgtgc cgttgtagat ctcgacggcc ttgttgttgc tcgaaccctc
    13321 gatgtactcc gagatgaaca ggtccgcggc ggagccgccg gcgccgacgg tgatcgtgaa
    13381 ggtctcgaag tctgcgccgt cgttgtccgc ggcgtagacg ctcatggtat agacgcccac
    13441 ggggccggcg ttggtccagc tgaacaggcc gttcgtgcca ctgctccaga acgtcgcggc
    13501 ggcaggcttg ttgctgatcg tcagggtgac cgcgtcgccg tcggtggccg cggcggtgac
    13561 ggcgaactga accgtgttgc tccaggtcgc cgacttgttg ccgatggagg cgagcacggg
    13621 cggcgtgttg gtcgggacgg tctccacggg cagcttcagg gcggggtcgc ccagcaggtt
    13681 gaggccgaac tggatccagc gctcggcctc gtccgtgccg cacgagctga tcatgtccgc
    13741 cttgtgcatg gcgaaggccg tgcccacggt gcggttggcg gtctcgtgga accgcttgta
    13801 gaacttgtac gcgtagatcg tggacgggcc gccgtccgac gtgttgctgg cgggggtttc
    13861 gtccggctcg ccccacccgt accgggcgca gcccatgtag gccacagcgc cgcccgcgtt
    13921 gcggtagttg cgcaggaagc cctcacccag gcagggttcc gtggtatagc cgtagccgtc
    13981 gatgttgttg ctgtcccggt cgaagtggcc ggtcaggcag gcgtccgtgt acacgaaggc
    14041 cgccatgccg gtcatggacg cggcgtcggt gttgtcgaag gacccggact ccagacccca
    14101 ctcctgcggc gcgccgtggc ccgagaacat caggtgcgtg tagttcttgt tgaacgcggc
    14161 cagcgtgttg gccgcgctct cggaatggtc gccgcaggtg ctgctgtccc acgaggtgat
    14221 cgtgtcgcag atgatgctca cggccccggg ccagtaggga tagatcccgt cgcggtagag
    14281 ccgccgcagc cacatctccg agtcgctgac gtaggtgtgc gcggggctgg tcgagcggaa
    14341 cccggcgtgg ccgtcgatgg tcacgtcgtc gctcggccgg gcggttcccg agtaggtgct
    14401 ccaggcctcc gggccgccga gcacgatctt gtccgcgagc cgcgagctgt ccgcgtcgta
    14461 cgcgatgacc ttgttgacgt agttggtcac ctgggcggcg gtccgcatcg ggatgcgggc
    14521 gacgatcacg tcccaggcca tgtccacgtt gtcgctggtc tcgccgtact tcgcgtcgct
    14581 gtcgctgttc cagttgccgc ccaggccgga atagtacagg tccgtcggca tgttggtctc
    14641 gtacgtgtcg cccgagacca cggccacgta gcagtcgcga tccggcacga tcgtgtcgtc
    14701 gccgcccagc accaccatcg tcgtgcccat cgtggccacg cggttgctga tgcaggcccg
    14761 gatcttctgc tggatgtccg cgccggtgaa ggtggagctg atgtaattgg tggtcagcac
    14821 cgtggtcgtg tagctgcccc ccgccgagga ggcgcggtag gtcgcgagct gctggaacgc
    14881 gttgctcaac gcgggcgacg tgatgatcag gtagtctagc gccgcccgcg tctccgtccg
    14941 ggacgcgggc gcgaactcgt cgctcgacga tgggttgacg accaggctgt tgaccagcgg
    15001 ctcgaactgg gccttctgct tcgcggagat cgtgcggggc gtcgccggag cgtcgtagga
    15061 gacggtgacg tcgatcgcgg ggcgcaggtg gagcgcccgg tccgcgccga cgtagaagag
    15121 gggattcacg cggacggaga caaaccggta gccctgcatg tcgtggatgc cctcgatcgt
    15181 cgcggcgtcc gccggccagg cttccgcgct ggcgtagcgg tcgttggcct ccgcgaaagc
    15241 cgggcgcggc ttgctcttcg ggctccgggg ctgcgaagga taggggagga tgtccgcggc
    15301 cagcagggcc ggttcgccga tggcccccaa cagcaccgcg ggcgccaggg cgcccaacgc
    15361 cgagcccgtc atcgaggcga aatagaccgg ccccggatgc tgggggtgaa ccacatacgc
    15421 cagcacaatg acccctccgg caaatacgaa aggcagaatc aa
//
LOCUS       DAOOTK010000074        15333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2608_length_15333_cov_5.418510, whole genome shotgun sequence.
ACCESSION   DAOOTK010000074 DAOOTK010000000
VERSION     DAOOTK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_15333_cov_5.418510"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PK634_09075"
     CDS             <1..1030
                     /locus_tag="PK634_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOW97927.1"
                     /translation="IAKYSQHEMDLLDLQANELVTRPVADAVADKQFPHLHLLVSVMQ
                     EGHLFDPFSREVPGAPSQMVFTFNNLIARTPFVPLVGRALAALEKAYGHPVDTEFTAH
                     VDAKGQVRINLLQCRPLWLPGAMGPVIWPEPVRPEQVIFRSDRFISGGEVDRIRFILY
                     IDPRRYAAMTDLSLKKSIGRVVGRINELPSVVEGRVLVLGPGRWGSSNIELGVNVGYA
                     DIDNAAVLVELAREEAGHVPEVSYGTHFFQDLVEQRIIYLPVYPDDPESSFNERFFES
                     APNALRDILPDAAPFEQVVKVIDVPSFARGSFVRVIADPDRRRALCYLPDLEAPRATA
                     AISDPSNF"
     gene            complement(1027..1641)
                     /locus_tag="PK634_09080"
     CDS             complement(1027..1641)
                     /locus_tag="PK634_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97928.1"
                     /translation="MFGNALAWLPILLLVFLLAFTPGIGFMEDLGRHLLLGRLILDRG
                     SVPDTNLLTYTWPDYPFINHHWLSEVVFYGLHRAVGLQRLPFWRRQVAADQLVYELYS
                     LACVNEFPRLWPALWELHRRYPDYQVVHELLRVCAPPEAVDAVKDVMARRARWPLAAK
                     QALDWGRVLEGEGRADEARAVYRRGRWFFPLSPDLARALAGPGR"
     gene            complement(1700..2398)
                     /locus_tag="PK634_09085"
     CDS             complement(1700..2398)
                     /locus_tag="PK634_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOW97929.1"
                     /translation="MSASRGTAILALLLLVPAASLGVASAMIFFPGTWLGPVIFGAAK
                     AWLFGLPLAWRLLVERQPISLSPPRHGGFAVAAMSGLAISLVIWAAYLVAGARLIDRG
                     FFVDKLTAVGLGSPAAYAGGAAYWILVNSVLEECVWRWFCVRQCEQLLPRGPAVILSG
                     LFFTVHHVVALRVYMGPIPTLLCSLGIFTGGAIWSWMYTRYRSIWPGYVSHAIVDLCV
                     FGLGAWLLFGPAAP"
     gene            complement(2395..3027)
                     /locus_tag="PK634_09090"
     CDS             complement(2395..3027)
                     /locus_tag="PK634_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97930.1"
                     /translation="MGQKLCPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYRYHSPL
                     VSGEWCAFTNYPWGRGLINFAPEEEAKAMETYGTWARLFELLRYYSWIVDRFHLSTRA
                     CQLQERRKDYDFRWLEQRLLPLGFRLVLCTRSPDSFRAARAERLKVSGKPSQYDDLDA
                     FVREQELLRRLASESILPRLELDVSGNSVPAACERIADWLEQTGGLYMAS"
     gene            complement(3051..3770)
                     /locus_tag="PK634_09095"
     CDS             complement(3051..3770)
                     /locus_tag="PK634_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOW97931.1"
                     /translation="MTAPSVLRALKRQAHPANVAGMARYGIRTRGALGVSIPALRAMA
                     KEIGQDHQLALDLWASGVHEARILAGLVADPAALTEREMERWARDFDSWDVCDQVCSN
                     LFDKTPSAWRKAVEWAGRDEEFVKRAGFVLMAALSVHDKAAPDSAFMKFMPLIRREAR
                     DERNFVKKAVNWALRQIGKRNERLRRAAVQTGKSIQRLDSRSARWIAADAIRELTSRR
                     PAKQKAQRTRGPSRTKTRMST"
     gene            complement(3767..3997)
                     /locus_tag="PK634_09100"
     CDS             complement(3767..3997)
                     /locus_tag="PK634_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HOW97932.1"
                     /translation="MDYDKDKVDEMTLALMCLVMSPGAGGGRAWKTFDWNTLVRLHRK
                     GWISEPKVKEISVAVTAEGLKKAGELFKKHFQ"
     gene            complement(4180..4255)
                     /locus_tag="PK634_09105"
     tRNA            complement(4180..4255)
                     /locus_tag="PK634_09105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4220..4222),aa:Ala,seq:cgc)
     gene            complement(4325..5530)
                     /locus_tag="PK634_09110"
     CDS             complement(4325..5530)
                     /locus_tag="PK634_09110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOW97933.1"
                     /translation="MNLNRVPPAPPDAILGLTEAFKKDPRPEKINLGVGVFMDDTGRT
                     PVLPTVKQAEEDLAREETSKSYLPIAGLPAFGERVADLVFGAGHEVVRSGRVRMAQSP
                     GGTGALRIGAEFLRKFLPSSKAWLSAPTWPNHKGVFSAAGLPVADYPYYDPASRSLAF
                     GKMAEALDSVPENDVVVLHVCCHNPTGADLSADQWREAAAIAARRRWIPFFDFAYQGF
                     GAGLEADRAGLLPFLQAGLPVLVAASFSKNFGLYNERTGALALVAPDAAEAAAWLSHV
                     QVLVRVLYSNPPAHGAAIAARILGDPLARADWEREVDAMRGRMVSIRDALVNGLRARG
                     APVDFSFIHAQRGMFSFSGLSDAQVAFLREKKAIYMVGGGRINVAGITTRNLDYLCDS
                     VVEALRAAP"
     gene            complement(5530..6708)
                     /locus_tag="PK634_09115"
     CDS             complement(5530..6708)
                     /locus_tag="PK634_09115"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOW97934.1"
                     /translation="MATELAFALINPYTISKSRTGGVIGRLLTRTGLELVAARMFGPS
                     QELAEKYAALLRGDPATEEEERQLLADYVQRCYAPDTKTGKPRRVLMLLFEGEDAIVK
                     IAKATGKVRHSTEASDTVRGTYGDFIRDEHGQVRHVEPAVMVGWTHDSAGAALRLWAD
                     YSERDGGLVEAAVDVYTGADHYQRTLVLIKPDNFRFPSARPGNIIDIFSGSGLRIIGA
                     KVHRMTVAEAEEFYGPVRQVLRAKLKGSVGQRAAAALAKELGFELPPDTVAQLGETLG
                     PMYGDDQFYKIIQFMTGQWGPACTLEEKQKKGKERCLALVYAGLNAVEKIRGILGPTD
                     PSKAQPGSVRKEFGQDIMVNAAHASDSPENAAREMKIIRVEDSMIREWVDRYYPAKGA
                     "
     gene            6820..8073
                     /locus_tag="PK634_09120"
     CDS             6820..8073
                     /locus_tag="PK634_09120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOW97935.1"
                     /translation="MNPSPHPGPRPDVAARQAALAAALAARIADAVAEGRPADAALQE
                     YFRAHPECGSRDRRFLGDLVFSFFRWRGWLPGTVQCKACALAWALDAEAPHPAGAALA
                     GDGTLFPAGRLTLSEKAALATRWLNRADPLRPENLVPAWVPGCLACEADRFLDAIQRR
                     PPTWLRVRRGREGVARAALEREAITVSAHPTVPSALAVPGSSPLPRLAADIRALFEVQ
                     DLASQVVGLLAAPRPGDHWGDACAGSGGKALHLADLMDDRGRISVSDVRADALRALAR
                     RAARAGFSILHPADPKRAGGPLLDGVVVDAPCSGLGTWSRNPDARWRTAWADVQRLAA
                     RQLDILSRAAMELKAGGRLVYSVCTLTRIETLDVTGQFLRAHPGFRLEPGPHPLNGAD
                     TDGRFWVWPWDGPCDGMYIARFRRG"
     gene            8157..9875
                     /locus_tag="PK634_09125"
     CDS             8157..9875
                     /locus_tag="PK634_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOW97936.1"
                     /translation="MLALLFLACCAALAAGYFIYGRFMERNLDVRADRVAPSVTLQDG
                     VDYVPTKTAVLFGHHFSSIAGAGPIVGPIIAGLAFGWFPALLWILVGSIFIGGVHDFT
                     ALIASLRHRARSIGEMCRDLLNPAAYYVFLIFIWLTLMYVQIVFMDLTASTFVPVTQP
                     ETADLQLHQGGVVATASMLYIGLAVLFGLSLYRFNVPVGKATLIFVPLVFLALWVSHL
                     LPLRADRIPEALLLGNAKNTWLAVLIVYCFLASILPVWILLQPRDYLSSFLLYACVGG
                     GVVGLLTSAAQGTVAIRYNPFSGWTHPELKGMFPALFITIACGAVSGFHSIVASGTTS
                     KQLSNEKSARTVAYGGMLVEAVLGLVALATVMVLADKPAGKTPVAIFAGGLGDFLAAL
                     HIPRQAAVTFGLMAVSSFLLTTLDTCTRLARFIFEEIFGLSGRLARYAGTAASLLLPA
                     LVVFRQIPGPGGKLMPAWQAVWPAFGATNQLLAALALLVVYAWLRRSGKRSGYVLLPM
                     LFMCVTTVTALTQLAILNLFHKGSLLVGAICLVLDLLAVALIISTAWNLLRRRPAPVG
                     FTPAPN"
     gene            10028..11002
                     /locus_tag="PK634_09130"
     CDS             10028..11002
                     /locus_tag="PK634_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOW97937.1"
                     /translation="MNIIQRAREVLDTESAALAKVRDGLGPAFEQAVRCLLDRLGRGG
                     KIVVTGVGKNQPIGQKIAATLTSTGAPSLFLHPSDAMHGDLGLLQANDALVVLSYSGA
                     SEELLALLPAVKRTGMPAIALTGDPESALSKLCDIVVPVQVDREACPFNMAPTASTTA
                     TLAVGDALAMVLLEARGFQLEDYAKLHPGGAIGRTLLMRVEDIMRTGERVAKVRPDTR
                     VQDAVLAMTAARSGSAVVVDDRDAVVGICTDGDLRRHIVDNSRNVASLNMADVMTREP
                     IRLTRGHLAVDVLAIFEQHNIDDLIIVDEGGRLMGMIDIQDLPKFKIF"
     gene            11014..12486
                     /locus_tag="PK634_09135"
     CDS             11014..12486
                     /locus_tag="PK634_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97938.1"
                     /translation="MARLPAFPAGALLEIRAAGLFGAAGFPLALRTLDAAGPPGAPCR
                     ALVLPPGAPEQGLPEGHIVLLRIRRGHLQEARQARQAALFRKLARRAAGIELGTESAL
                     ADAWATFFDPGRGWIGIVEEWIDGRYWKMETDDRLWDRPVPSDHPERDDPAAFRTEYD
                     AKRLFMARLERLFQRLGAADLARQYRWSTWISQRNALKRIDAAASPHAGWTAVDFPGT
                     ALYLPALRQYVESRADHFVDLLPLLRDMEAGRLPAPGTVRVGLPEDRPAPVPERRAGL
                     VHAALRKLAFSWHMLFRPEMRNARLMEEVQEGLAQGMLTPAEAERIRGQVHDPMLQTY
                     LTCLAVHLAMLPLTNLTVLAGAAWYSLTRDLSWAEGARLAAYGLAFFAVFPMSPGSLA
                     RGLFVVAVALKRRSARRLRIALALSFWRYVGYMAFPLQMVSAFPALARYLAARWATRA
                     VRAIPVYGQPGALLEHRMFDLAFNWPLTLARGWRSRRPVS"
     gene            12564..13163
                     /locus_tag="PK634_09140"
     CDS             12564..13163
                     /locus_tag="PK634_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97939.1"
                     /translation="MKTLRGVQGAFGFGLLLGVFSAAAVEVDWAFHSASGEDNRAIQF
                     VPGQAFTFLGNGKTEVPDDDGVTIYVMTANQFAGDAEEQVFVRWWNGEREFWVIADWF
                     ANAILDVTGENAGVFRGKPEFGSVMVDIWKVYIPPEYTRRGDNFYVVQLKAFSPQGSI
                     ENYLLHDADLEGARVNNLGQAWTGNPDYHDHDWKVVIME"
     gene            complement(13230..14636)
                     /gene="ahcY"
                     /locus_tag="PK634_09145"
     CDS             complement(13230..14636)
                     /gene="ahcY"
                     /locus_tag="PK634_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOW97940.1"
                     /translation="MAKKFSDFRVKDLRLADFGRKEIELAEHEMPGLMALREEFGKKQ
                     PLRGARITGSLHMTIQTAVLIETLQHLGARVRWASCNIFSTQDHAAAALVTRGTPVFA
                     VKGETLEEYWEYTDRILDWGNGQGPNMILDDGGDATLYVHLGCQAEDNPKILDRKPGS
                     HEEKILLAQLKKAIRRNPGRFHRIVKGIRGVSEETTTGVHRLYKMHEKGELLFPAFNV
                     NDSVTKSKFDNIYGCRHSLVDGIMRATDVMVSGKVAVVAGYGDVGKGSCQALKGQCAR
                     VIVTEIDPICALQAAMEGYEVMTMDEACRLGDIFVTATGCCDVITEQHMRRMKNLAIV
                     CNIGHFDHEIQVEPMRKYRWEEIKPQVHRIHMPSGRSIILLAEGRLVNLGCATGHPSF
                     VMSNSFTNQTMAQIELYSNPGKYPVGVYVLPKHLDEKVARLHIGKIGVKLEKLSRKQA
                     AYLGVPVNGPYKPGHYRY"
     gene            complement(14793..>15333)
                     /locus_tag="PK634_09150"
     CDS             complement(14793..>15333)
                     /locus_tag="PK634_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97941.1"
                     /translation="DISPSMVNKGVVQDVTQQTVTMMEAMHPGTRFNLIVFVDGALAF
                     APQMVFATQENKQKALAWLQKGFDSHREGNRPGYSGSTSSEAIRLAAEMGCDTMFVLS
                     DDPPYLKTGNMETGVEIPDHQDQILAFVRSIESNTGRPTKINPILYKPFKNERGEKAK
                     TFYQTVARTTGGRMKVIDD"
BASE COUNT         2341 a         5264 c         5086 g         2642 t
ORIGIN      
        1 gatcgccaag tactcgcagc acgagatgga cctgctcgac ctgcaggcca acgagctggt
       61 cacgcgcccc gtcgccgacg ccgtcgcgga caagcagttc ccccaccttc accttctcgt
      121 ctcggtgatg caggaaggcc acctcttcga tcccttcagc cgcgaggtgc ccggcgcgcc
      181 gtcgcagatg gtcttcacct tcaacaacct gatcgcgcgt acccccttcg tccccctcgt
      241 cggccgggcg ctggccgcgc tggaaaaggc ctacgggcac cccgtggaca ccgagttcac
      301 cgcccacgtg gacgcgaagg gccaggttcg gatcaacctg ctgcagtgcc gcccgctctg
      361 gctgccgggc gcgatgggcc ccgtaatctg gccggagccg gtccggccgg agcaggtcat
      421 cttccggtcc gaccgcttca tcagcggcgg cgaggtggac cgcatccgct tcatcctcta
      481 tatcgaccct cgccgctacg ccgcgatgac cgacctgtcg ctgaagaaga gcatcggccg
      541 cgtagtgggc cggatcaacg agctgccctc cgtcgtggaa ggccgcgtcc tcgtgctcgg
      601 gcccggccgc tggggcagca gcaacatcga gctgggcgtc aacgtcggct acgcggacat
      661 cgacaacgcc gcggtcctcg tcgagctggc gcgggaggag gcgggccacg tccccgaggt
      721 ctcgtacggc acgcatttct tccaggatct cgtcgagcag cggatcatct atctccccgt
      781 ctaccccgac gacccggagt cgtccttcaa cgagcgcttc ttcgaaagcg cgcccaacgc
      841 gctgcgggat atcctgcccg acgccgcgcc cttcgagcag gtcgtcaaag tgatcgacgt
      901 gccgtccttc gcgagggggt cgttcgtccg cgtgatcgcc gatccggatc ggcgccgagc
      961 cctgtgctac ctgccggacc tggaagcgcc gcgcgccacc gccgccattt cggatccgtc
     1021 gaatttctag cggcccggtc ccgccagggc ccgggccagg tcgggcgaca gggggaagaa
     1081 ccagcggccc cgccggtaca cggcgcgcgc ctcgtccgcg cggccctcgc cctccagcac
     1141 gcggccccag tccagcgcct gcttcgccgc caacggccaa cgggcccgcc gggccatgac
     1201 atccttcacc gcgtccacgg cctcgggcgg ggcgcagacg cgcagcagtt cgtggacgac
     1261 ctggtagtcc gggtaccgtc ggtgcagctc ccacagcgcc ggccagagac gcgggaactc
     1321 gttgacgcag gccaggctgt acagttcgta cacgagctgg tcggccgcga cctgtcggcg
     1381 ccagaacggc aggcgctgca gtccgaccgc ccggtgcagg ccgtagaaca ccacctccga
     1441 cagccagtga tggttgatga acgggtagtc cggccatgtg tacgtcagca ggttggtatc
     1501 cggcacgctc ccccgatcga ggatgagccg gcccaacagg agatgccgcc ccagatcctc
     1561 catgaagccg atgcccggcg tgaaggccag gaggaacacg agcagcagga tcgggagcca
     1621 cgcgagggcg tttccaaaca ttggaaaaaa gcatcgcaca tttttccaaa cattggaaaa
     1681 cattccgcgc gtccgacggt cacggcgccg ccggcccgaa cagcagccac gcgccgagcc
     1741 cgaagacgca caggtccacg atcgcgtggc tcacgtagcc cggccagatg gaccggtacc
     1801 tcgtgtacat ccatgaccag atcgcgccgc ccgtgaagat gcccagcgag cagagcagcg
     1861 tcggaatcgg tcccatataa acccgcaacg ccacgacatg gtgcacggtg aagaacagcc
     1921 ccgagaggat caccgccggc ccgcgcggga gcagttgttc gcattggcgg acgcaaaacc
     1981 agcgccagac gcactcctcc agcacggagt tcaccagtat ccagtaggcc gcgccgccgg
     2041 cgtacgcggc cggcgagccc aggccgacgg ccgtcagctt atcgacgaag aatccccggt
     2101 cgatcaaccg cgcgcccgcg acgagatagg ccgcccagat gaccagcgag atcgccagcc
     2161 ccgacatcgc cgccaccgcg aagccgccgt gccgcggcgg cgagaggctg atcggttggc
     2221 gttcgacgag cagcctccag gccagcggca ggccgaacag ccaggctttc gcggcgccga
     2281 aaatgacggg gcccagccac gtgccgggga agaagatcat cgcggacgcc acacccagac
     2341 tcgccgcggg cacgagcagc agcagggcca ggatggctgt gccccggctt gcgctcatga
     2401 agccatgtac aggccgccgg tctgctcgag ccagtcggcg atgcgctcgc aggcggcggg
     2461 cacgctgttg ccggacacgt cgagttccag gcgcggcagg atggattccg aggccagccg
     2521 gcgcagcagt tcctgctcgc ggacgaaggc gtccaggtcg tcatactgcg acggcttccc
     2581 ggacaccttc aggcgctccg cgcgcgccgc ccggaacgaa tcgggcgagc gcgtgcacag
     2641 gaccagccgg aacccgagcg gcagcaaccg ctgctccagc cagcggaagt cgtagtcctt
     2701 gcggcgctcc tgcagttggc aggcccgcgt ggacaggtgg aaccggtcga cgatccacga
     2761 gtagtagcgc agcagttcga agagccgcgc ccaggtcccg tacgtctcca tggccttggc
     2821 ctcttcctcg ggcgcgaaat tgatcaagcc ccgaccccac gggtagttcg taaacgcgca
     2881 ccattcgccc gagaccagcg gcgagtgata gcggtacttg cgcggcccga cgatgcgggg
     2941 atgctcgttg agcgcaaagg caatgtccgt cttgaaggtc agccgggtgc cttccaggat
     3001 gattttagga cagagttttt ggcccatata gagtgcggag ggttcagggt tcaagtcgac
     3061 attcgcgtct tagtccggct cggtcctcgc gtcctttgcg ccttctgttt cgccggcctg
     3121 cgtgacgtca gttcccggat tgcgtcggcg gcgatccagc gggcgcttcg cgaatccagg
     3181 cgttgtatgg atttgcccgt ttggacagcg gcccggcgca ggcgttcatt gcgcttgccg
     3241 atctgccgca gcgcccagtt gacggccttc ttcacgaagt tccgttcgtc gcgggcctcg
     3301 cgccgaatca ggggcatgaa tttcatgaag gcggagtccg gcgcggcctt gtcgtggacc
     3361 gacagggcgg ccatgaggac gaagcctgcc cgcttgacga attcctcgtc gcggccggcc
     3421 cattccacgg ccttccgcca ggccgaagga gtcttgtcga acaggttcga gcagacctgg
     3481 tcgcagacgt cccacgaatc gaagtcgcgg gcccatcgtt ccatctcgcg ctcggtcagc
     3541 gcggcgggat cggcgaccag tccggccagg atgcgggcct cgtgaacccc cgacgcccac
     3601 aggtcgaggg ccagttgatg atcctgcccg atttccttcg ccatcgcgcg gagcgcgggg
     3661 atggacacgc cgagcgcgcc ccgggtgcgg atgccatagc gggccattcc ggccacgttg
     3721 gcgggatggg cctgacgctt cagggcgcga agaacggacg gggcggtcat tggaagtgct
     3781 ttttgaacaa ttccccggcc ttcttcaagc cttccgccgt gacggcgacc gagatctcct
     3841 tgaccttggg ctcggagatc cagcccttcc ggtgaaggcg aaccagcgta ttccagtcga
     3901 aggtcttcca ggcccgcccg ccccccgccc ccggcgacat gaccaggcac atgagcgcca
     3961 gggtcatttc gtcgactttg tccttgtcgt agtccatggt cccgccccca tcttcacgca
     4021 cccatcagca tggtagcaga tgtccgtcgc gggaaaagac cagatcaggc aaaatggcag
     4081 ggaagcgcga gacggaatcg aaggtgttct ccgtgcgact gcccgtctcg ccaagcccac
     4141 ggcgggcatc tttcgcctcc gcgttgctgc ggcgaaggct ggtggagcgg aagggagttg
     4201 aaccctcggc ctccagagtg cgattctggc gctctcccaa ctgagctacc gccccatctt
     4261 ccgtgaagac ccgcacaatc tacgtgctcc cctatccctt cgcaagcgtt ttctcccgag
     4321 gcgctcacgg tgcggcgcgc agggcctcga ccacggaatc gcagaggtag tccaggttac
     4381 gggtcgtaat gccggccacg ttgatccggc cgccgccgac catgtagatg gccttcttct
     4441 cccgcaggaa cgcgacctgc gcgtcggaga gcccgctgaa ggagaacatg ccccgctggg
     4501 cgtgaatgaa ggagaaatcc accggggcgc cgcgcgcgcg caacccgttc accagcgcgt
     4561 cgcggatgga caccatccgc ccgcgcatgg cgtccacttc ccgctcccag tcggcccgcg
     4621 cgagcgggtc gccgagaatc ctcgccgcaa tcgcagcgcc gtgcgcgggg ggattggaat
     4681 aaaggacgcg gacgaggacc tggacatggg acagccacgc cgccgcttcc gccgcgtccg
     4741 gcgcgaccag cgccagggcg cccgtgcgtt cgttgtaaag cccgaagttc ttggagaacg
     4801 aggccgcgac caggaccggc aggccggcct ggaggaacgg cagcaggccc gcgcggtcgg
     4861 cctcgaggcc cgcgccgaaa ccctggtagg caaaatcgaa gaacgggatc cagcgccgcc
     4921 gcgccgcgat cgccgccgcc tcgcgccact ggtccgcgga caggtccgcc cccgtggggt
     4981 tatgacagca gacgtgcagg accacgacgt cgttttccgg cacgctgtcc agcgcctcgg
     5041 ccatcttccc gaacgccagg ctgcgggagg cgggatcgta ataggggtaa tcggccacgg
     5101 ggagcccggc cgcggaaaaa acgcccttgt gattgggcca cgtcggcgca ctcaaccagg
     5161 cctttgacga aggcaggaat ttccgcagga attccgcgcc gatgcggagc gcgcccgtcc
     5221 cgccgggcga ctgggccatg cgcacgcggc cggaccggac gacctcgtgc ccggccccga
     5281 agaccaggtc cgccacgcgt tcgccaaagg ccggcaggcc ggcgatgggc aggtaggact
     5341 tggaggtctc ctcgcgcgcc aggtcttcct cggcctgctt gacggtgggg agcaccggcg
     5401 tgcggccggt gtcgtccatg aacaccccga cgcccaggtt gatcttctcc gggcggggat
     5461 cttttttgaa cgcctccgtc aggccgagaa tggcatccgg cggcgccggc gggacccggt
     5521 tcaagttcat caggcgcctt tcgcgggata gtagcggtcc acccactcgc ggatcatact
     5581 gtcctcgacg cggatgatct tcatttcccg cgcggcgttc tcgggcgagt cggaggcgtg
     5641 ggccgcgttg accatgatat cctgcccgaa ctccttgcgg accgagccgg gctgggcctt
     5701 gctgggatcc gtgggcccga ggatcccgcg gatcttctcc accgcgttca gcccggcgta
     5761 cacgagggcc aggcagcgtt ccttgccctt cttctgtttc tcctccagcg tgcaggcggg
     5821 gccccactgg ccggtcatga actggatgat cttgtaaaac tggtcgtcgc cgtacatcgg
     5881 gccgagggtc tcgcccaact gcgccacggt atccggcggc agctcgaagc ccagttcctt
     5941 ggcgagggcc gccgccgcgc gctgcccgac cgaacccttg agcttggccc ggagcacctg
     6001 gcggacgggc ccgtagaatt cctcggcctc cgcgaccgtc atgcggtgga ccttcgcgcc
     6061 gatgatgcgc aggccggagc cggagaagat gtcgatgatg ttgcccggcc gcgcgctggg
     6121 gaagcggaaa ttatccggct tgatgagcac cagcgtgcgc tggtaatggt ccgcgcccgt
     6181 atacacgtcc acggccgcct cgaccaggcc gccgtcccgc tcgctgtagt cggcccagag
     6241 ccgcagcgcg gcgcccgctg aatcgtgcgt ccaacccacc atcacggccg gctcgacatg
     6301 gcgcacctgg ccgtgctcgt cgcggatgaa gtccccgtag gtgccgcgca cggtgtcgga
     6361 ggcctccgtg ctgtgccgca ccttgccggt ggccttcgcg atcttgacga tggcgtcctc
     6421 gccctcgaac agcagcatca gcacgcgccg cggtttgccg gtcttcgtgt cgggcgcgta
     6481 gcagcgctgc acgtagtccg ccagcagttg ccgctcctcc tcttccgtcg ccgggtcgcc
     6541 ccgcagcagg gcggcgtact tctccgccag ctcctggctg ggcccgaaca tgcgcgcggc
     6601 gaccagttcc aggccggtgc gggtcaagag ccggccgatg acgccgccgg tgcgggactt
     6661 cgagatcgtg tacgggttga tcaatgcaaa ggctaattct gtcgccatgg cgttctcctg
     6721 ctgttcccgt gggatgttat ggagatgccg cccggaatca agcccgaagg aagcgcgcgg
     6781 ggctgcaagt ccgggccgga tatattatag tcggtgcgga tgaatccaag cccccacccc
     6841 ggcccgcgcc cggatgtagc ggcccggcaa gccgcgctcg cggccgcgct agcagcgcgg
     6901 atcgccgatg ccgtggcgga gggccggccg gccgacgccg cgctccagga atatttccgc
     6961 gcgcacccgg aatgcggctc gcgcgaccgg cgattcctgg gcgacctcgt cttctctttc
     7021 ttccgatggc ggggctggtt gcccggaacg gtgcaatgca aggcttgcgc cctcgcctgg
     7081 gcgctcgacg cggaggcgcc gcaccccgcg ggcgcggcgc tggcgggtga cggcacgctg
     7141 ttcccggccg gtcggctcac cctgtcggaa aaggccgccc tggcgacgcg ctggctgaat
     7201 cgggccgatc cgctccggcc cgaaaacctg gtccccgcgt gggtcccggg ctgcctggcc
     7261 tgcgaggccg atcggtttct cgacgccatt caacgccggc cgccgacctg gctgcgcgtg
     7321 cggcggggcc gggaaggtgt cgcgagggcc gcgctggaac gggaggcgat aactgtctcc
     7381 gcccatccca ccgtgccctc ggccctggcc gtgccgggtt cgtcgcctct gccgcgactc
     7441 gccgcggaca tccgcgcgct gttcgaggta caggacctcg cctcgcaggt cgtcgggctg
     7501 ctggccgcac cccggccggg cgaccactgg ggcgacgcgt gcgcgggttc gggcggaaag
     7561 gccctccatc tcgcggacct catggacgac cggggccgga tttccgtatc ggacgtccgg
     7621 gccgacgcgc tccgcgcgct ggcccgccgc gcggcgcggg caggattttc gatccttcat
     7681 ccggcggacc cgaaacgggc cgggggcccg ctcctggacg gcgtcgtcgt ggacgccccc
     7741 tgctccgggc tgggcacgtg gtcgcggaat cccgacgcgc gctggcgcac ggcgtgggcc
     7801 gatgtccagc ggctggccgc ccggcaactg gacatcctct cgcgggccgc gatggaattg
     7861 aaggccggcg gacgcctggt ctattcggtc tgcaccctga cccggatcga gaccctggac
     7921 gtcaccggcc agttcctccg cgcccatccc ggattccgcc tggagccggg tccgcacccg
     7981 ctgaacgggg cggacacgga cggccggttc tgggtgtggc cgtgggacgg cccctgcgac
     8041 gggatgtaca tcgcccgctt ccggcggggt tgagttccgc cccttcgctc ttgccgccgc
     8101 cgggctttgt gctaacgttc ccctgtccgg catgttccgg tgggagacgc actcgcatgc
     8161 tcgcattact cttcctggcc tgttgcgccg cgctggcggc cggctatttc atctacgggc
     8221 ggttcatgga gcggaacctg gacgtccggg ccgaccgtgt cgccccgtcg gtcacgttgc
     8281 aggacggggt ggactatgtt ccgacgaaga cggccgtgct cttcggccat cacttcagtt
     8341 ccatcgccgg cgccggcccg atcgtcgggc ccatcatcgc cggtctcgcc ttcggctggt
     8401 tccccgcgct gctctggatc ctggtcggtt ccattttcat cgggggcgtg cacgacttta
     8461 ccgcgctcat cgccagcctg cgccaccggg cgcgttcgat cggcgaaatg tgccgcgacc
     8521 tgctcaatcc cgcggcctac tacgtgttcc tgatcttcat ctggctgacg ctgatgtacg
     8581 tccagatcgt cttcatggac ctcacggcgt ccacgttcgt gccggtgacg cagccggaga
     8641 cggccgacct ccagctgcac cagggcggtg tcgtggccac ggcctccatg ctctacatcg
     8701 gcctcgccgt gctcttcggg ctttcgctct accggttcaa cgtgccggtg ggcaaggcca
     8761 cgctgatctt cgtgccgctg gtgtttctgg ccctctgggt cagccacctg ctgccactcc
     8821 gggcggaccg gattccggag gcgctgctcc tgggcaacgc caagaacacg tggctggcgg
     8881 tgctcatcgt ctactgcttc ctcgcctcga tcctgcccgt gtggatcctt ctccagccgc
     8941 gcgactacct ttcctccttc ctcctgtacg cctgcgtcgg cggcggggtc gtcggcctgc
     9001 tgacgtccgc ggcgcagggc acggtcgcga tacggtacaa tcccttctcg ggctggaccc
     9061 atcccgagtt gaaggggatg ttccccgccc tcttcatcac catcgcctgc ggcgcggtca
     9121 gcgggttcca ttccatcgtc gcgtccggga ccacgtccaa gcagctctcg aacgagaagt
     9181 ccgcgcgcac ggtggcctac gggggcatgc tggtcgaggc cgtcctcggc ctggtggccc
     9241 tggcgacggt catggtcctg gccgacaagc ccgcgggcaa gacgccggtg gccatcttcg
     9301 cgggcgggct gggcgatttc ctggccgcgc tgcatatccc ccgccaggcg gccgtcacgt
     9361 tcgggctgat ggcggtcagt tcgttcctgc tgacgacgct ggacacctgc acgcggctgg
     9421 cccggttcat cttcgaggag atcttcggct tgagcgggcg cctggcccgc tacgccggga
     9481 cggcggccag cctgctgctg cccgcgctcg tggtcttccg ccagatcccg gggcccggcg
     9541 ggaagctcat gcccgcgtgg caggcggtat ggccggcctt cggggccacc aaccagctcc
     9601 tcgcggcgct cgcgctgctg gtggtgtacg cgtggctgcg tcgcagcggc aagcgcagcg
     9661 ggtacgtcct gctgcccatg ctgttcatgt gcgtcacgac ggtcacggct ctcacgcaac
     9721 tcgccatcct gaacctcttc cacaagggca gcttgctcgt cggcgcgatc tgcctggtgc
     9781 tggacctgct ggccgtcgcg ctgatcataa gcaccgcctg gaacctcctg cgccgccggc
     9841 ccgcccccgt cgggttcacg ccggctccga attgaaccga aggcgcgaag ggcgccagga
     9901 gggcctcgaa aatagggctg gagatttctt ccaaacattg gaaaaatcgg cccatggttt
     9961 ttccaaacat tggaaaaaac cgcggctttt tttccaaaca ttggaaaaag cggaaggaga
    10021 gcgtttgttg aatatcatcc aacgcgcccg cgaggtcctg gataccgaga gcgccgccct
    10081 ggccaaggtc cgcgacgggc tgggcccggc cttcgagcag gccgtccgat gcctgctgga
    10141 ccggctcggt cgcggcggca agatcgtcgt taccggcgtg ggcaagaacc agccgatcgg
    10201 ccagaagatc gccgcgacgc tcacgagcac cggggcgccg tccctcttcc tgcatccctc
    10261 cgacgccatg cacggggacc tgggcctgct gcaggcgaac gacgccctgg tcgtcttgag
    10321 ctatagcggc gcctccgagg aactgctggc cctgctgccc gccgtcaagc gcaccggcat
    10381 gccggccatc gcgctgaccg gcgacccgga aagcgccctg tccaagctgt gcgacatcgt
    10441 cgtgccggtc caggtggacc gcgaagcctg cccgttcaac atggcgccca cggccagcac
    10501 gaccgccacg ctcgccgtcg gcgacgccct ggccatggtc ctcctcgagg cccgcggctt
    10561 ccagctggag gactacgcca agctccaccc cggcggcgcc atcgggcgca cgctgctgat
    10621 gcgcgtggag gacatcatgc ggaccggcga gcgcgtggcg aaggtccggc ccgacacccg
    10681 cgtgcaggac gccgtgctgg cgatgaccgc cgcccgctcc ggctcggccg tggtggtgga
    10741 cgaccgcgac gcggtggtcg gcatctgcac cgacggcgac ctgcggcggc atatcgtcga
    10801 caactcccgc aacgtggcgt cgctgaacat ggcggacgtg atgacccgcg agccgatacg
    10861 gctgacccgc gggcacctgg ccgtggatgt gctggccatc ttcgagcagc acaacatcga
    10921 cgatctcatc atcgtggacg agggcgggcg cctgatgggc atgatcgaca tccaggacct
    10981 gcccaagttc aagatcttct gaccgcgggt ccgatggcgc gactcccggc atttcccgca
    11041 ggggcgctcc tggaaatcag ggccgccggt ctcttcggcg cggccgggtt cccgctcgcg
    11101 ttgcgcacgc tggacgccgc cgggccgccg ggggcgcctt gccgggcgct cgtgctgccg
    11161 cccggcgccc cggaacaggg gcttcccgag ggccacatcg tcctgctgcg aatccggcgc
    11221 ggccatcttc aggaggcgcg gcaggcccgg caggccgcgc tgttccgcaa gctggcgcgg
    11281 cgcgccgccg ggatcgaact ggggacggaa tccgcccttg ccgacgcgtg ggccacgttt
    11341 ttcgatcccg ggcgcggctg gatcggaatc gtggaggaat ggatcgacgg ccgctactgg
    11401 aaaatggaga cggacgaccg tttgtgggat cgccccgtcc cctccgacca tcccgagcgg
    11461 gacgatccgg ccgccttccg aaccgaatac gatgccaagc gcctcttcat ggcgcggctg
    11521 gagcgcctgt tccagcgcct cggcgccgcc gacctggccc gacaatatcg gtggtccacg
    11581 tggatcagcc agcggaatgc gctcaagcga atcgacgcgg ccgcctctcc gcacgcgggc
    11641 tggaccgccg tggatttccc ggggaccgcg ttatacctgc ccgcccttcg ccagtacgtc
    11701 gagagccggg ccgaccattt cgtggacctg ctgccgctcc tgcgggacat ggaggccggg
    11761 cgactccccg cgccgggtac ggtccgggtg gggcttcccg aggacaggcc cgcgccagtt
    11821 ccagagcgac gcgcggggct tgttcacgcc gcgcttcgca agctggcgtt ttcctggcac
    11881 atgctcttcc gccccgagat gcgcaacgcc cggctgatgg aagaagtcca ggagggactg
    11941 gcccagggca tgctgacgcc ggccgaggcg gagcggatac gcgggcaggt ccacgatccg
    12001 atgctccaga cctacctgac ctgcctggcc gttcatctgg cgatgctccc gctcacgaat
    12061 ctcaccgtgc tcgccggggc cgcctggtac tctctgacgc gggacttgag ctgggccgag
    12121 ggcgcgcgtc tggcggccta cggcctggcg ttcttcgcgg tcttcccgat gtcgccgggc
    12181 tcgctcgcgc gcgggctctt cgtcgtggcc gtcgccctga agcgccgctc cgcccggagg
    12241 ctccggatcg cgctggccct ctccttctgg cgctacgtcg gctacatggc tttcccgctg
    12301 cagatggtca gcgcgttccc agcgctcgcg cgctacctgg ccgcccgctg ggccacccgc
    12361 gccgtccgcg cgatccccgt gtacggccag cccggtgccc tgctggaaca ccggatgttc
    12421 gacctggcct tcaactggcc gctgaccctg gcaagggggt ggagaagtcg tcggccggtt
    12481 tcttgaaatg gcggaccaag cctgctattc ttccagtcgt tcatccgtcg ccgttgccgg
    12541 cgatgcgtag taaaggagta tgcatgaaga cgttgcgagg ggtgcagggc gcctttggat
    12601 tcggcctgct gctcggagtt ttctccgcgg ccgcggtgga ggtggactgg gcttttcatt
    12661 cggccagcgg ggaggacaac cgggccatcc aattcgtccc cggccaggcg ttcacgttcc
    12721 tgggcaacgg caagacggaa gttcccgacg atgacggggt cacgatctac gtgatgaccg
    12781 ccaaccagtt tgccggtgac gcggaggagc aggtcttcgt gcgctggtgg aatggcgagc
    12841 gcgagttctg ggtcatcgcg gattggtttg ccaacgcgat cctggacgtg acgggggaga
    12901 acgcgggcgt gttccgcggc aagccggaat tcggcagcgt gatggtggac atctggaagg
    12961 tctacatccc cccggaatac acccggcgcg gcgacaactt ctacgtggtg cagctcaagg
    13021 ccttcagccc gcagggctcc atcgagaact acctgctgca cgacgcggac ctcgagggcg
    13081 cccgcgtcaa caacctgggc caggcctgga ccgggaaccc ggattaccac gatcacgact
    13141 ggaaggtcgt gatcatggaa taagtccggc cgcacaccat ggtgcggcgc cgctggttcg
    13201 gtagcgcgcc acggcgtggc gcgcggggat cagtaccggt agtgtcccgg cttgtaaggg
    13261 ccgttcaccg gcacgccgag gtaggcggcc tgcttcctgg agagcttctc cagcttgacc
    13321 ccgatcttgc cgatatgcag ccgcgcgacc ttctcgtcaa ggtgcttcgg cagcacgtac
    13381 acgcccaccg ggtacttgcc cgggttggag tagagttcga tctgggccat ggtctggttg
    13441 gtgaagctgt tggacatcac gaagctgggg tggcccgtgg cgcagcccag gttgaccagc
    13501 cggccctcgg ccagcaggat gatgctccgg ccgctgggca tgtggatccg gtgcacctgc
    13561 ggcttgatct cctcccagcg gtacttgcgc atgggctcga cctggatctc gtggtcaaaa
    13621 tggccgatgt tgcagacgat ggccaggttc ttcatccggc gcatgtgctg ctcggtgatc
    13681 acgtcgcagc agcccgtggc cgtgacgaag atgtcgccga ggcggcaggc ctcgtccatg
    13741 gtcatgacct cgtagccttc catcgcggcc tgcagggcgc agatcgggtc gatctcggtc
    13801 acgatcacgc gggcgcactg gcccttgagg gcctggcagc tccccttccc cacgtccccg
    13861 tagccggcga cgacggcgac cttgccggag accatgacgt ccgtggcgcg catgatgccg
    13921 tcgacgagcg aatgccggca gccgtagatg ttgtcgaatt tcgacttggt gaccgagtcg
    13981 tttacgttga aggccgggaa cagcagctcg cccttctcgt gcatcttata caggcgatga
    14041 acgcccgtag tggtctcctc gctgacgccc cggatgccct tgacgatccg gtggaagcgg
    14101 ccgggattcc ggcggatcgc cttcttcaac tgggccagca ggattttctc ctcgtggctt
    14161 cccggcttgc ggtcgaggat cttcgggttg tcctcggcct ggcaaccgag gtgcacgtac
    14221 aaggtggcgt cgccgccgtc gtcgaggatc atgttcgggc cctggccgtt cccccagtcg
    14281 aggatgcggt ccgtgtattc ccagtattcc tccagcgttt cgcccttgac ggcgaagacg
    14341 ggggtgccgc gcgtgacgag cgcggcggcg gcatgatcct gggtggagaa gatgttgcag
    14401 ctggcccagc gcacgcgggc gccgaggtgc tggagggtct caatcaggac cgcggtctgg
    14461 atggtcatgt gcagcgagcc ggtgatccgg gcgccacgca ggggctgttt tttcccgaac
    14521 tcctcgcgca gggccatgag gcccggcatt tcgtgctcgg ccagttcgat ctccttgcgg
    14581 ccgaaatcgg caagccggag gtcctttacg cggaaatcgc tgaacttctt cgccatggtt
    14641 tacccttccg ttttgtggtc tgaagggcgg cattgtgcat gaatccacgc gtatttccag
    14701 tctttttaga gcccccgcgc cagtgacatg ttttataaaa catgtcaccg caaagggagg
    14761 actgacccgg ccgagagaag cggcgagggg aatcaatcgt cgatgacctt catccgcccg
    14821 ccggtggtgc gggctacggt ctggtaaaac gtcttggcct tttcgccccg ctcgttcttg
    14881 aagggcttgt acaggatcgg gttgattttc gtggggcgtc ccgtattgct ttcgatcgac
    14941 ctcacgaacg ccagaatctg gtcctggtgg tccgggattt ccacgccggt ctccatgttg
    15001 ccggtcttca agtatggagg atcatcggag aggacgaaca tcgtgtcgca gcccatctcg
    15061 gccgccagcc ggatggcctc ggaggacgtg ctgccggaat agcccggccg gttcccctcg
    15121 cggtgcgagt cgaatccctt ctggagccac gccagggcct tctgcttgtt ttcctgcgtg
    15181 gcgaacacca tctgcggcgc gaacgccagc gccccatcca cgaacacgat gaggttgaag
    15241 cgggtgccgg ggtgcatggc ttccatcatc gtgacagtct gctgcgtcac atcctggacg
    15301 acccccttgt tgaccatgct ggggctgatg tcc
//
LOCUS       DAOOTK010000075        15328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2610_length_15328_cov_5.684148, whole genome shotgun sequence.
ACCESSION   DAOOTK010000075 DAOOTK010000000
VERSION     DAOOTK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15328
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_15328_cov_5.684148"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="atpD"
                     /locus_tag="PK634_09155"
     CDS             complement(<1..902)
                     /gene="atpD"
                     /locus_tag="PK634_09155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOW97942.1"
                     /translation="MTISQTSQPAGRIAQILGAVVDVEFGGDRLPAIFTALRTTNPAI
                     DDRPGNLVLEVAQHLGSGVVRTVAMAATDGLVRGAPVEDTGREMSMPVGPATLGRVLN
                     LLGEPVDGLGPVRSDQRLPIHRPAPALGNQGTSETPLVTGIKVIDLLTPYKKGGKIGL
                     FGGAGVGKTVLIQELIHNIATQHGGYSVFAGVGERTREGTALHLEMKESGVLDKTSLV
                     FGQMNEPPGARARVALSALTVAEYFRDEMRQDVLLFIDNIFRFTQAGSEVSALLGRIP
                     SAVGYQPTLGTDMGELQERITSTK"
     gene            complement(914..1786)
                     /gene="atpG"
                     /locus_tag="PK634_09160"
     CDS             complement(914..1786)
                     /gene="atpG"
                     /locus_tag="PK634_09160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOW97943.1"
                     /translation="MASYRIYNARLASLRSMRRVTKTMKMVSATKLRRAQAAEERATR
                     FSGRMGDVAGHVLALPEARAHEFVLPRAPVRNGLLIVLSADKGLCGGFNANLVRFAAQ
                     WIEEQRARFHKLRAAFCGRRALAALRDRVEVRNVYDGVAARPDFRAALRIGRDVTDAF
                     AARQYDEVHLAYNRFHSAMSQEPVIERLLPVPLDTAAAPAAPGPRAEHLFEPEAPRLI
                     ELFLRQLVCLRLHSALLQNAAGEHGARMTAMDSATSNVDNLTLRYTKLRNQARQSSIT
                     GELIEIISGAEALK"
     gene            complement(1801..3315)
                     /gene="atpA"
                     /locus_tag="PK634_09165"
     CDS             complement(1801..3315)
                     /gene="atpA"
                     /locus_tag="PK634_09165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOW97944.1"
                     /translation="MSTDLKAQEIAAILKRQLTDAELKPDVSEVGTVLSIGDGIARVF
                     GLNRAMAGELVEFSGGVTGLVLNLEEENVGVAVFGEDQQVREGDEVRRTHRITSVPVG
                     GPVAGRVVDALGRPLDDGGPIAAEEFRPVEVRAPGIIERQNVREPLQTGIKIIDALTP
                     IGRGQRELIIGDRKTGKTTLAIDAIINQRGQDVACFYVAIGQKRSTVAQIHEALRQHK
                     AMPYTTIVAATASDPAPMQFLAPYSACTMAEYYRDSGRHALIVYDDLTKQAQAYRQMS
                     LLLRRPPGREAFPGDIFYLHSRLLERAAKMSDALGGGSLTALPIVETQANDIAAYIPT
                     NVISITDGQIFLEADLFNRGQRPAVNPGISVSRVGGDAQVKAMKQVSGSLRIDLAQYR
                     EMEAFAQFSTDLDAATLKQLERGRRLMELMKQEARATLPAAVQVAVIYAGVHGHLDAV
                     PLARAGAYESELREALGSLYADYLRLFEEHKALTPEVTKALDALLANFGRRFKP"
     gene            complement(3337..3876)
                     /gene="atpH"
                     /locus_tag="PK634_09170"
     CDS             complement(3337..3876)
                     /gene="atpH"
                     /locus_tag="PK634_09170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOW97945.1"
                     /translation="MKPTRDIRTLAKTLFRLAEAQGLSERLRGELDAVTALFDACPEW
                     RLFVGQLALGQARRQSVLRELFEPRAHPLLLRFLLAVNGQGHLRRLPDIAREFDALFR
                     RSRGILDAEIVSAEPLDVAQAARLRERLAARLGGTVETTLSVNPALLGGFQVRVGDEV
                     RDFSVAARLRSMRRALAQA"
     gene            complement(3881..4462)
                     /gene="atpF"
                     /locus_tag="PK634_09175"
     CDS             complement(3881..4462)
                     /gene="atpF"
                     /locus_tag="PK634_09175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOW97946.1"
                     /translation="MKSGFHPPLPLAIAPAAPHQAVAEVPSAPPQQERSEMDVSGRMV
                     ILTWISFLTAAYLLYKLAWKPILAALEKREETIRKSLQDVEFAKRQVESLQAKQKQIV
                     AEADRQAAEILDQSRNAAQGLAAQVETKAREEARELLDQATREIREARDRVIRDLRRE
                     SADLALTLAEKLVRRNLDSETQRELVRKLTDEV"
     gene            complement(4491..4703)
                     /gene="atpE"
                     /locus_tag="PK634_09180"
     CDS             complement(4491..4703)
                     /gene="atpE"
                     /locus_tag="PK634_09180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOW97947.1"
                     /translation="MTAGLALASIGAGLATVGVGLGIGRVSASAVEGIARQPEAAPKI
                     QITMLIAAALIEGFGLFAAVICFLQI"
     gene            complement(4734..5612)
                     /gene="atpB"
                     /locus_tag="PK634_09185"
     CDS             complement(4734..5612)
                     /gene="atpB"
                     /locus_tag="PK634_09185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOW97948.1"
                     /translation="MTAQFGRFSWGGTGGLLSCTVSLDVTSAVAAAETSVETRTAVHD
                     YIVRHAMYHMAESDSWNLPYVHLRFLDIFRYDATMVIVSALLLVAAVSLIWRRRRDPP
                     RGMTALLEFYVLFIRDKICGAFIGDRLGRRLTPYFCTLFLFILTMNLLGLVPLFSAAT
                     NNINVTGALALLTLGLMLVGSVFENGLRGPLKAMAPPGVPWPLLIILVPVEFISTLGK
                     AFALMIRLFANILAGHVIIFCLLGLVVILGYAALPAILLAIVLFFFELFVCFFQAYIF
                     TLLSAIFIGQFVHAEH"
     gene            complement(5669..5932)
                     /locus_tag="PK634_09190"
     CDS             complement(5669..5932)
                     /locus_tag="PK634_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97949.1"
                     /translation="MPTTHPRDRSATGKMLGLTMSLLSFMAVAVLGLAWLGAAVGARC
                     GHRRGGALAGALLGFFLGLHELWNMVRAIERESRSKDAPAPGP"
     gene            6033..6743
                     /locus_tag="PK634_09195"
     CDS             6033..6743
                     /locus_tag="PK634_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOW97950.1"
                     /translation="MNTTRPNHLALIKQFLANRQDVEIIETGYPFVTISRQSAAGGHT
                     LARCILQEMDGYADEELLQGWDMFDEKLCAIVAVDDVNSASYESLVTEEYKSDLHHAI
                     YDMIVGRSEQFSTYKKIFEILRALAKLGKVIVIGRAGAFVTAGMPAGIHLRLVADEKD
                     RVRWAMERLSVDEAKAAETVRRQDRDRERLVREFFNRDVNNPVHYDAVFNTSRMSHED
                     LAAIIVRMIRSRALKNGK"
     gene            6754..8439
                     /locus_tag="PK634_09200"
     CDS             6754..8439
                     /locus_tag="PK634_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOW97951.1"
                     /translation="MDDNLINTATSIVAAAAAPAAPANPHELMTLLVLQLAVIIIAAK
                     LGGLLFRRFLRLPSVLGELVAGMLIGPYALGAVIPVPGIGPLFALTNPHYPVSSELQA
                     ISQIAAIILLFLSGLDTNLTVFLRYSVVGSLVGLGGVIFSFVLGDLCAVFFGVAHSFR
                     DPQALFLGTISTATSVGITARVLAEKRKMSSPEGVTILAGAVIDDVLGIIVLAVVIGM
                     TTLTRSGEQVHWGSISMVAVKAVGFWVICMTVGLLLARRLTGTLKHLRSPETIAGVSL
                     GLALLLSGFSEMAGLAMIIGAYIMGLSLSRTDIVSLLQSHLQGLYDMLVPIFFCVMGM
                     LVDVRATQQVLVFGLVYSLLAIIAKVVGCALPAYATQFNAHGALRIGLGMVPRGEVAL
                     IVAGIGLSSHTIGQDVFGVSILMTMITTFLAPPLLERSFARGGTGLKREVSAAEQEGT
                     RSFQLEFPSADLAAFVLERLAQAFRDEDFYVFRLGQETQTYQIRKEAMVFSLSQEGQT
                     ILLTASEVNEHVARFIVLEELLELQDMARAFERLRDMDDMKNSLLKGLFDGGN"
     gene            8477..9289
                     /locus_tag="PK634_09205"
     CDS             8477..9289
                     /locus_tag="PK634_09205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOW97952.1"
                     /translation="MDFLLYIKAAILGVVEGATEFIPVSSTGHLIVAQNLLGFTGAKE
                     NAFIIFIQLGAILAVVWLYRRRIGEWVWNGDRAGEAGRLLLNLFVATLPAVAIGLPTH
                     AWIEAHFFRPLPVALALIGGGAAILLVERFHRPTEVEVLERIPLRTAFGVGLIQVLSI
                     LFPGVSRSGATILGGMALGLSRPAAAEFSFFLALPAMAGSSLVELAGAREALAWSDAP
                     LFVLGFAVSFAVALAVIRALLAFVARHSFVPFAWYRFAAGAAVLAWLVLAHP"
     gene            9307..9984
                     /locus_tag="PK634_09210"
     CDS             9307..9984
                     /locus_tag="PK634_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOW97953.1"
                     /translation="MNSPEQKKAPRGLARHLRRRLVSGVLLLVPLAITLFILNLIFNA
                     LTAFALPLLRPWLGEWPEYALVAVALAVTFALIYLVGLIATHIIGRRIIQFGEAILLK
                     LPIVKSVYSASKQVVNTFSASTAAAFQSVVLVEFPRTGSLAVGFVTGTILDPGERRLY
                     RVFVPTTPNPTSGFLVLLPAEQVRFTDISVEDGVKMIVSGGMLSPERYAETPPPAAGE
                     VERPSSP"
     gene            complement(9998..11224)
                     /locus_tag="PK634_09215"
     CDS             complement(9998..11224)
                     /locus_tag="PK634_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOW97954.1"
                     /translation="MKGITHFLTGVATASCFPLAIRTAYDDKAYLMVLGGVCGILCDT
                     LDFKFARYFWKHDYQIRLTEDNLDPRLPAEAIARAIDEAAEKKRPVRLKLDTIRVSGS
                     YYRTYAVQIDDRNRTVTCTIGPLKTMSQSMARGERLPSETIRRRKIEEDGPAAFMESL
                     ARNLPALPDTIPPGPTSHVATFKTEINNTYYMDTEIGVFSGPDYEFVPEPDGRVRVDF
                     IPWHRRWTHSLTLGLCLGPVGFALFADWPALFRGDLAAFANPFAVNAFFIAILAFWSH
                     VLVDQTGFLGSNLFPPFTHKRTMGLRWSTSASPFPNQMVNYISLCIILWNINVFAPVP
                     VFTMPWAREMTGGFADSAYYLVSLANYLLAVVAVPLLVFYWIGKLYKRLYRRDTQVQM
                     EEAFTGEDWSGGAGDG"
     gene            complement(11241..12578)
                     /locus_tag="PK634_09220"
     CDS             complement(11241..12578)
                     /locus_tag="PK634_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOW97955.1"
                     /translation="MELNLVLMCIPTALLGVAVAAVFSFIPGLHIYNVIAFTMMLAFA
                     FVEFFAALDPLLTTSFILGMVVGFSVLFTVSSQFFQPCDDSFRSMMMPHERFLYEGRG
                     YEAVILAGFGSLIALVLIAGLFPFLTKPIGLVRELIRPHVYWILGLVSAFILIGEWPK
                     DHGVGKTVRQRLADGWVQLGMGFFTFVAAAVLGMFVFYNTIIPVESAFQSLMPVFMGL
                     FALPSQIMTVITRATIPPQHFAGSVDAEPHDLARGALTGTVAGLFSSLTPGMTPGPAL
                     LVAGHATASSGDKQFIVGGGAARVLYYIGALVLFFMPDVYMRRGGAAINISLFFVPET
                     VEQYLAVSGVIALSGATALWLLPRYARFCARTTQRVNYHWISAASVLLLVAMVLFITG
                     WRGLVLMAVSTALGLVPNFWHTRRVPLLAVLMVPVSLNMAGVGHQVAQWLGFR"
     gene            complement(12582..13430)
                     /locus_tag="PK634_09225"
     CDS             complement(12582..13430)
                     /locus_tag="PK634_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97956.1"
                     /translation="MPGPEPLLCPSCGRPLPAESGVCRFCAASLRTRISTAWIGRGAL
                     LLLVIAAIHFTAALQYRPRLTALGDLTAEMNFERVRVAGRVVNVNVTQDRYNAPLVRL
                     DLEDLATADAPARQRLSVRLEGEAALDFVALKDPPRRGDVIEVAGSLFAGSSYRFISV
                     NSASLLKIKSRPAGSMPEAREDMPATIAELLAEPGRFSNRWIVVAAAEVADVAATHPI
                     VRIREPGQTNTVMVFGFDGRSLTVGQKVAVRGHFVYFEKMGYWEIMTARGNPRAIEPV
                     AAAGGD"
     gene            complement(13417..14340)
                     /locus_tag="PK634_09230"
     CDS             complement(13417..14340)
                     /locus_tag="PK634_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97957.1"
                     /translation="MRLIKLDPEISRLVMSQNPAETTCRACGRYIGSWERCPFCRQFN
                     PKRRIVRLLKYSTPFLTIAGIVLLSHMGRVYGSPTVKISSLGRKTNFAQVRVEGRISG
                     DLRYYPADAKGRNSSLEFEVDDGTGLMRIRCYDDTTEELLKADKIPVFGDRVSLIGNY
                     QYKARRQIMILSAVEDLDIVREKPGEATPIARVVRAFREGLEEGTRLKVTGRIQSRIP
                     SSYDMVWLIEDEGGNRLAVNLSQDVVKARRAEQAGAQLEQMREGSFITCYGALQRYRS
                     QKQSAWQLALAGPEDIQPADEAQWRADNAGP"
     gene            14536..>15328
                     /locus_tag="PK634_09235"
     CDS             14536..>15328
                     /locus_tag="PK634_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97958.1"
                     /translation="MGWWKQQRRRLEVGLARLAMGVVNRLPRPVVLGLARFLGAAGYL
                     LARSLRRIGLANLDLAYGEALTGREKRRLLKRSFQSFALVTLDVFWFSRRTEQRIRRW
                     VSFERPLSEVLGRGANVCLTGHLGNWEVLGLACAVEGYPLASVAMPLKNPDVDVLFNR
                     LRQSTGQTIIPREGAVRALLRLLREGRNAALLLDQNTRPEHGGLFVDFFGRPATVSGA
                     AVFLAARAGCGIVSGFCLPDGRGGYRVRLPDGLGVPPPKPDDTRVR"
BASE COUNT         2373 a         5098 c         5332 g         2525 t
ORIGIN      
        1 ttcttcgtgg acgtgatccg ctcctgcagt tcgcccatgt ccgtgcccag cgtgggctgg
       61 tagccgaccg cggaggggat gcggccgagc agcgcggaga cctccgagcc cgcctgggtg
      121 aagcggaaga tgttgtcgat gaacagcagc acgtcctgcc gcatctcgtc gcggaagtac
      181 tccgcgaccg tgagcgccga gagcgcgacg cgcgcgcggg cgcccggcgg ctcgttcatc
      241 tggccgaaca ccagcgaggt cttgtcgagg acgcccgact ccttcatctc gaggtggagg
      301 gcggtgccct cccgcgtgcg ctcgccgacg ccggcgaaca cggagtagcc gccgtgctgg
      361 gtcgcgatgt tgtggatgag ttcctggatc agcacggtct tgcccacgcc cgcgccgccg
      421 aacaggccga tcttaccgcc cttcttgtac ggggtgagca ggtcgatcac cttgatgccc
      481 gtgaccagcg gcgtttccga ggtgccctgg tttcccagcg cgggcgcggg acggtggatc
      541 ggcagccgct ggtccgaccg gacggggccg agcccgtcca ccggctcgcc gagcaggttc
      601 aggacgcggc cgagcgtcgc cggcccgacc ggcatgctca tctcccggcc ggtgtcttcg
      661 accggcgcgc cgcggacgag gccgtcggtg gccgccatgg ccacggtgcg cacgaccccg
      721 ctgccgaggt gctgcgcgac ctcgagcacc aggttgccgg gccggtcgtc gatcgcgggg
      781 ttcgtcgtcc gcagggcggt gaagatggcg gggaggcggt ccccgccgaa ctccacgtcc
      841 accacggcgc ccaggatctg ggcgatccgt cccgcgggct ggctggtctg tgatatcgtc
      901 atggcgttcc gtcctacttt agcgcttcgg cgccggagat gatctcgatc agttccccgg
      961 tgatggaaga ctgccgcgcc tggtttcgca acttggtgta gcggagggtc aggttgtcca
     1021 cgttgctcgt ggcgctgtcc atggccgtca tccgcgcccc gtgctcgccc gccgcgttct
     1081 ggaggagggc cgagtggagg cgaaggcaca cgagctggcg gaggaacagc tcgatgagcc
     1141 ggggcgcctc gggctcgaac aggtgctccg ccctcgggcc cggcgcggcg ggcgccgccg
     1201 cggtgtccag cgggaccggc agcaggcgct cgatgaccgg ctcctggctc atggcgctgt
     1261 ggaagcggtt gtacgccagg tggacctcgt cgtactggcg ggccgcgaag gcgtccgtca
     1321 cgtcccgccc gatgcggagc gcggcccgga aatcggggcg ggccgcgacg ccgtcgtaga
     1381 cgttgcggac ctcgacgcgg tcccggagcg cggccagggc gcggcggccg cagaacgcgg
     1441 cgcgcagctt gtggaaccgc gcccgctgct cctcgatcca ctgggcggcg aaacggacca
     1501 ggttggcgtt gaacccgccg cacaggccct tgtcggccga gaggacgatc agcaggccgt
     1561 tgcggaccgg cgcgcgcggg aggacgaact cgtgagcgcg ggcctcgggg agggcgagga
     1621 cgtgtcccgc gacgtcgccc atccggccgg agaaccgcgt ggcccgctcc tccgcggcct
     1681 gggcgcgccg cagcttggtc gccgagacca tcttcatggt cttggtcacc cgccgcatgc
     1741 tgcgcaggct ggccagcctg gcgttgtata tccggtagct cgccatggtc gcgcgccgtc
     1801 ctacggcttg aaccgccgcc cgaagttcgc caggagcgcg tcaagcgcct tggtgacctc
     1861 gggcgtcagg gccttgtgct cctcgaacag ccgcaggtaa tccgcgtaga gcgagcccag
     1921 cgcctcgcgc agttccgact cgtaggcgcc cgcgcgggcg agcggcacgg cgtccaggtg
     1981 gccgtggacg ccggcgtaga tcacggcgac ctgcacggcc gcggggagcg tggcccgggc
     2041 ctcctgcttc atcagctcca tcagccggcg gccgcgctcg agctgcttga gggtggccgc
     2101 gtccaggtcc gtcgagaact gggcgaaggc ctccatctcc cggtattggg ccaggtcgat
     2161 gcggagcgag ccggagacct gcttcatggc cttgacctgc gcgtcgccgc cgacgcgcga
     2221 gacggagatg ccgggattga cggccggccg ctggccgcgg ttgaacaggt ccgcctcgag
     2281 gaagatctgg ccgtccgtga tcgagatgac gttggtcgga atgtacgcgg cgatgtcgtt
     2341 ggcctgggtc tcgacgatcg gcagggcggt caggctgccg ccgcccaggg cgtcgctcat
     2401 cttcgcggcg cgctcgagga gccggctgtg gagatagaag atgtcgccgg ggaacgcctc
     2461 gcgcccgggc ggccgccgca ggagcagcga catctggcgg taggcttggg cctgcttggt
     2521 caggtcatcg tagacgatca acgcgtgccg tccgctgtcc cggtaatact ccgccatggt
     2581 gcacgcgctg tacggggcca ggaactgcat cggcgccggg tcggaggccg tggcggcgac
     2641 gatggtggtg tagggcatgg ccttgtgctg gcggagcgcc tcgtggatct gcgccaccgt
     2701 ggagcgcttc tggccgatgg ccacgtagaa acaggccacg tcctggccgc gctggttgat
     2761 gatcgcgtcg atggccagcg tggtcttgcc cgtctttcgg tcgccgatga tcagctcgcg
     2821 ctgcccgcgc ccgatcgggg tcagggcgtc gatgatcttg atgccggtct gcaggggctc
     2881 gcggacgttc tgccgctcga tgatgcccgg cgcccggacc tcgacgggcc ggaactcctc
     2941 ggcggcgatc ggcccgccgt cgtccagcgg gcggccgagc gcgtccacca cgcggccggc
     3001 gaccgggccg ccgacgggca cggaggtgat ccggtgcgtc cgccgcacct cgtcgccttc
     3061 gcggacctgc tggtcctcgc cgaacacggc gacgccgacg ttctcctcct cgaggttcag
     3121 gaccaggccg gtcacgccgc cggaaaactc caccagctcg cccgccatcg cgcggttcag
     3181 gccgaacacg cgggcgatgc cgtcgccgat gctcaagacg gtgccgactt cgctgacgtc
     3241 cggcttgagc tcggcgtcgg tcagctgccg cttgaggatc gccgcgatct cctgggcttt
     3301 caggtctgtg gacatggaag gcttctctcc cttctcctac gcttgggcca gggcccgccg
     3361 catcgaccgc agccgggccg ccacgctgaa atcgcgcacc tcgtcgccga cccggacctg
     3421 gaacccgccg agcagggcgg ggttcacgga cagcgtcgtc tcgaccgtgc ccccgaggcg
     3481 ggcggccagc cgctcccgca ggcgcgcggc ctgcgcgacg tccagcggct cggccgagac
     3541 gatctcggcg tccaggatgc cgcgcgaacg ccggaagagg gcgtcgaact cccgcgcgat
     3601 gtccggcagc cgccgcaggt ggccctgccc gttcaccgcg agcaggaacc gcagcagcag
     3661 cggatgcgcc cggggctcga acagctcgcg gaggacggac tgccgccggg cctgtccgag
     3721 ggcgagttgg ccgacgaaga gccgccactc ggggcaggcg tcgaagaggg cggtcaccgc
     3781 gtccagttcg ccgcggaggc gttcggacag gccctgcgcc tccgccaggc ggaagagggt
     3841 tttcgcgagg gtccgtatgt cccgggtcgg tttcaagggg ctacacttcg tccgtaagtt
     3901 tccgcaccag ttcgcgctgg gtttcgctgt ccaggttccg gcgcacgagt ttctccgcga
     3961 gcgtcagggc caggtcggcg ctttcgcggc ggaggtcgcg gatcacgcgg tcgcgcgcct
     4021 cgcgaatctc ccgcgtcgcc tggtccagca gctcccgggc ttcctcccgg gccttcgtct
     4081 ctacctgggc cgccaggccc tgggcggcgt tccggctctg gtcgaggatc tccgcggcct
     4141 gccggtcggc ttccgccacg atctgctttt gcttggcctg cagcgactcg acctgccgct
     4201 tcgcgaactc gacgtcctgc agggatttcc ggatcgtctc ctcccgtttc tccaacgcgg
     4261 cgaggatggg cttccacgcc agcttgtaca ggagataggc ggcggtcagg aagctgatcc
     4321 aagtcaggat caccatgcgg cccgagacat ccatctcgct gcgctcctgc tggggcggcg
     4381 cggaggggac ttcggcgacg gcctggtgcg gcgcggccgg cgcgatggcc agcgggagag
     4441 gcggatgaaa acctgatttc acggcatggg ttttcggcgt gacatctttc ctagatttgc
     4501 aggaaacaga tcacggcggc aaacagcccg aatccctcga tgagcgccgc ggcgatcagc
     4561 atggtgatct ggattttcgg cgccgcttcc ggctggcgcg cgatcccctc caccgcggag
     4621 gccgagacgc ggccaatccc caggcccacg ccgacggtgg ccaatccggc cccgatgctg
     4681 gccaatgcca atcctgctgt catggtgtac ctcttttctt cgctgccttt tactcaatgt
     4741 tccgcgtgca cgaactgccc gataaagatg gccgacaaga gggtgaaaat gtaggcctga
     4801 aagaagcaca caaagagctc gaagaagaac agcacaatgg ccagcaagat ggccggaagg
     4861 gccgcgtagc cgaggatgac gaccaggccc aggaggcaga agatgatgac gtggccggcc
     4921 aggatattgg caaacagacg gatcatcagc gcaaacgcct tgccgagcgt gctgatgaac
     4981 tccaccggca ccaggatgat caggagcggc cagggaacgc ccgggggcgc catggccttg
     5041 agcgggcccc ggagcccgtt ttcaaagacg ctgccgacga gcatcaaccc gagcgtcagg
     5101 agggccagcg cgccggtgac gttgatgttg ttggtcgcgg cgctgaacaa gggcacgagt
     5161 ccgagcaggt tcatggtcag gatgaagagg aagagggtgc agaaataggg cgtcaggcgc
     5221 cggcccaggc gatccccgat gaaggccccg cagatcttgt cccggataaa caacacgtag
     5281 aactccagga gggccgtcat gccccgcggc ggatcccggc gccggcgcca gatgaggctg
     5341 accgccgcca cgagaaggag ggcggacacg atcaccatgg tcgcgtcgta cctgaagatg
     5401 tccaggaagc gaaggtggac atacggcagg ttccaggagt ccgactcggc catgtggtac
     5461 atggcgtggc ggacaatgta gtcatggacg gctgtcctgg tttcgacaga cgtttccgcc
     5521 gccgccacgg ccgatgtgac atccaggctc acggtgcatg aaagaaggcc gccggttccc
     5581 ccccaggaga accggccgaa ttgagcggtc atcatacgct ccgggaatcc ccccacaatt
     5641 aaaaaaagaa gctgtacagg gccttgaatc aaggcccggg tgcgggggcg tccttcgagc
     5701 gggattcgcg ctcgatcgcg cggaccatgt tccagagttc gtgaaggccc aggaagaatc
     5761 ccagcagggc gccggccagg gcgccgcccc ggcgatggcc gcagcgggcg ccgacggcgg
     5821 ctcccagcca ggccagcccc agtacggcca cggccatgaa agagagcagg gacatggtca
     5881 gccccagcat ctttcccgtc gcggaccggt ctctgggatg agtcgtgggc atgaacggct
     5941 tccactgtac ccggcgtcgg ccgtggggcc ataaaaaaaa cgtgtccagg ggccgggcca
     6001 tgatatgatg cacgttcatc gcaatactgg ccatgaatac gacgcgcccg aatcatctgg
     6061 ccttgatcaa gcagttcctg gccaaccgcc aggacgtcga gatcatcgaa accggttacc
     6121 ccttcgtgac gatctcgcgg caatccgccg cgggcggtca cacgctggcc cgctgcatcc
     6181 ttcaggaaat ggacgggtat gccgacgagg aactcctcca gggctgggac atgttcgacg
     6241 agaaactttg cgccatcgtg gccgtggacg acgtgaacag cgcgtcgtat gaatcgctgg
     6301 tgacggagga atacaagtcg gaccttcacc acgcgatcta cgacatgatc gtcggccgct
     6361 ccgaacagtt ttccacgtac aaaaagatct tcgagatcct gcgcgccctc gcgaagctgg
     6421 gcaaggtgat cgtcatcggg cgggccggag cgttcgttac ggccggcatg ccggccggta
     6481 ttcatttgcg cctggtcgcg gacgagaagg accgggtccg gtgggcgatg gagcggttga
     6541 gcgtggacga agccaaggcc gcggagacgg tccgccgcca ggaccgcgac cgggagcggc
     6601 tggtccggga gtttttcaac cgggacgtga acaacccggt tcattacgac gccgtcttca
     6661 acaccagccg gatgagccac gaggacctcg cggcgatcat cgtccggatg atccgaagca
     6721 gggccctgaa aaacggcaaa tagaaaaggt cgcgtggacg acaacctgat caatacggca
     6781 acgagcatcg tggcggccgc cgccgcgccg gcggcgcccg ccaatcccca cgagctgatg
     6841 acgctcctcg tcctgcagct ggcggtgatc atcatcgccg ccaaactggg cgggctgctc
     6901 ttccggcgtt tcctgcgcct gccgagcgtg ctgggcgagc tggtggcggg catgctgatc
     6961 ggcccgtacg cgctgggcgc cgtcatcccc gtccccggga tcgggcccct gtttgcgctg
     7021 accaacccgc attaccccgt ttcctcggaa cttcaggcga tctcccagat cgccgcgatc
     7081 atcctgctct tcctgtccgg cctggacacg aacctgaccg tgttcctgcg ctactccgtg
     7141 gtcggctcgc tggtggggct gggcggcgtg atcttctcct tcgtcctcgg cgacctctgc
     7201 gccgtgtttt tcggcgtcgc gcactcgttc cgcgacccgc aggccctgtt tctcggcacg
     7261 atctccacgg ccacctcggt cggcatcacg gcgcgggtcc tggccgagaa gcgcaagatg
     7321 tcgtcgccgg agggcgtgac gatcctcgcg ggggcggtca tcgacgacgt gctcggcatc
     7381 atcgtgctgg cggtcgtgat cggcatgacc accctgacgc ggtccggaga gcaggtccac
     7441 tggggctcga tctcgatggt ggcggtcaag gcggtgggct tctgggtgat ctgcatgacc
     7501 gtcggcctgc tcctcgcgcg ccgattgacg ggcacgctca agcacctgcg gtccccggag
     7561 accattgccg gcgtgtcgct cggcctggcg ctgctgctct ccggcttctc cgagatggcc
     7621 ggcctggcga tgatcatcgg ggcgtacatc atggggctct ccctctcgcg cacggacatc
     7681 gtgagcctgc tgcagagcca cctgcagggg ctctacgaca tgctcgtgcc catcttcttc
     7741 tgcgtcatgg gcatgctggt ggacgtgcgg gcgacgcagc aggtgctggt cttcgggctg
     7801 gtgtactcgc tgctggcgat catcgcgaag gtggtcggct gcgccctccc ggcctacgcg
     7861 acgcagttca acgcccacgg cgccctccgg atcgggttgg gcatggtgcc ccgcggcgag
     7921 gtggccctga tcgtcgccgg catcggcctc tcctcgcata ccatcggcca ggacgtcttc
     7981 ggcgtgtcga tcctcatgac catgatcacg accttcctcg cgccgccgct gctggagcgc
     8041 tccttcgccc ggggcggcac ggggctcaag cgcgaggtct ccgccgcgga acaggagggg
     8101 acgcgcagct tccaactgga gttcccttcc gcggacctcg cggccttcgt gctggagcgg
     8161 ctggcccagg cgttccgcga cgaggacttc tacgtcttcc ggctgggcca ggaaacccag
     8221 acctaccaga tccgcaagga agcgatggtc ttctcgcttt cacaggaggg gcagacgatc
     8281 ctcctcacgg cgtcggaggt taatgagcac gtcgcccgct tcatcgtgct cgaggaactg
     8341 ctcgaactgc aggacatggc ccgggccttc gaaagactgc gcgacatgga cgacatgaaa
     8401 aattcgctgc tcaagggcct tttcgatggc ggcaactaga cgcgcgggcc ccggctgacc
     8461 gggtcgcgct cctcccatgg acttcctcct ctatatcaag gcggcgatcc tcggcgtggt
     8521 cgagggcgcc acggagttca tccccgtctc gtcgaccggc cacctgattg tcgcgcagaa
     8581 cctgctcggc ttcaccggcg cgaaggagaa cgccttcatc atcttcatcc agctcggcgc
     8641 gatcctggcc gtcgtgtggc tgtaccggcg gcggatcggc gaatgggtct ggaacggcga
     8701 ccgcgccggg gaggccgggc ggctgctgct gaacctgttc gtggccacgc tgcccgcggt
     8761 cgccatcggg ttgccaaccc atgcgtggat cgaggcgcat ttcttccggc cgctgccggt
     8821 ggccctggcg ctcatcggcg gcggcgcggc gatcctgctg gtcgaacggt ttcaccgccc
     8881 gacggaggtg gaggtcctgg agcggatccc gctgcggacg gccttcgggg tggggctgat
     8941 ccaggtcctg tccatcctgt tccccggcgt ttcgcgctcg ggcgcgacga tcctgggcgg
     9001 gatggccctg gggctgtcgc ggccggcggc cgcggagttc tccttcttcc tggcgctgcc
     9061 cgcgatggcc ggctcgtcgc tggtcgaatt ggcgggggcg cgggaggcgc tggcgtggag
     9121 cgacgcgccg ctcttcgtgc tggggttcgc ggtgtccttc gccgtggcgc tggcggtcat
     9181 ccgggcgctg ctggccttcg tcgcccggca ttcctttgtc ccgttcgcct ggtaccgttt
     9241 cgccgcgggc gcggccgtgc tggcctggct tgtcctcgcg catccttgaa aggaacagcc
     9301 gcctccatga attcccccga gcagaagaag gcgccgcgcg ggctggcccg tcacctccgg
     9361 cggcggctcg tttcgggcgt gctgctcctc gtgccgctcg cgatcacgct gttcatcctg
     9421 aacctcatct tcaacgcgct cacggccttc gcgctgcccc tgctgcggcc gtggctgggc
     9481 gagtggccgg agtacgcgct ggtcgcggtc gcgctggcgg tgaccttcgc gctgatctac
     9541 ctggtggggc tgatcgccac gcacatcatc ggccgccgga tcatccagtt cggcgaggcc
     9601 atcctgctga agctgccgat cgtgaagtcc gtgtactcgg cgtccaagca ggtggtgaac
     9661 acgttctccg cctccaccgc ggccgccttc cagtcggtgg tgctggtgga atttccgcgc
     9721 acgggctccc tggccgtcgg cttcgtcacc ggcacgatcc tcgatcccgg ggagcggcgg
     9781 ctctatcggg tgttcgtccc gacgacgccg aacccgacct ccggcttcct ggtgctccta
     9841 cccgcggagc aggtccggtt cacggatatc tccgtcgagg acggcgtgaa aatgatcgtg
     9901 tccggcggga tgctgtcgcc ggagcgctac gccgagacgc cgcccccggc ggcgggcgag
     9961 gtcgagcggc cctcttcccc ttagccggcc gggcccgtca gccgtcgccc gcgccgccgg
    10021 accagtcctc gcccgtgaag gcctcttcca tctggacctg cgtgtcgcgg cggtacagcc
    10081 gcttgtacag cttgccgatc cagtaaaaga ccagcaaggg cacggcgacg acggccagca
    10141 ggtagttggc cagcgagacg aggtagtagg ccgagtcggc gaacccgccg gtcatctccc
    10201 gcgcccaggg cattgtgaaa accggcacgg gcgcgaacac gttgatgttc cagaggataa
    10261 tgcagagcga gatgtagttg accatctggt tcgggaatgg cgaggcggac gtgctccacc
    10321 gcaggcccat ggtccgctta tgcgtgaagg gcgggaagag gttggagccc aggaagccgg
    10381 tctggtccac gaggacgtgg ctccagaagg cgaggatggc gataaaaaac gcgttgacgg
    10441 cgaacgggtt ggcgaacgcg gcgaggtcgc cccggaacag cgccggccag tccgcgaaga
    10501 gcgcgaagcc gaccgggccc aggcagaggc caagggtcag gctgtgggtc cagcggcggt
    10561 gccaggggat gaagtccacg cgcacccgcc cgtccggctc cggcacgaac tcgtagtcgg
    10621 gcccggagaa cacgccgatc tcggtgtcca tgtagtaggt gttgttgatc tcggtcttga
    10681 acgtggccac gtggctggtc gggccgggcg ggattgtgtc cggcagcgcc ggcaggttcc
    10741 gggctagcga ctccatgaag gctgcggggc cgtcctcctc gatcttgcgc cggcggatgg
    10801 tctcgctcgg caggcgttcg ccgcgggcca tggactggct catggtcttg agcgggccga
    10861 tggtgcaggt gacggtccgg ttccggtcgt cgatctggac ggcgtacgtg cgatagtagc
    10921 ttccggacac gcggatcgtg tcgagcttca gccggaccgg gcgcttcttt tccgccgcct
    10981 cgtcgatcgc ccgcgcgatg gcctcggcgg ggaggcgcgg atccaggtta tcctccgtca
    11041 gccggatctg gtagtcatgc ttccagaagt agcgggcgaa cttgaagtcc agcgtgtcgc
    11101 agaggatgcc gcagacgccg ccgaggacca tgaggtaggc cttgtcgtcg tacgcggtcc
    11161 ggatggcgag cgggaagcag gaggccgtcg ccacccccgt caggaaatgc gtaatgcctt
    11221 tcatcgccct cgctcctcgc tcaccggaac ccgagccatt gcgcgacctg gtggccgacg
    11281 ccggccatgt tgaggctcac cgggaccatc agcaccgcca gcagcggcac gcggcgcgtg
    11341 tgccagaaat tgggcaccag gccgagcgcg gtcgagaccg ccatcagcac gagcccgcgc
    11401 cagcccgtga tgaacaggac catggccacc agcagcagca cggaggcggc cgagatccag
    11461 tggtaattca cgcgctgggt cgtccgcgcg cagaagcgag cgtagcgcgg cagcagccag
    11521 agcgccgttg ccccgctcaa cgcgatcacc cccgacacgg ccaagtactg ctcgacggtc
    11581 tcgggcacaa agaagaggct gatgttgatc gcggcgccgc cccggcgcat gtacacgtcc
    11641 ggcatgaaga acaggaccag cgcgccgatg tagtagagga cacgcgcggc gccgccgccg
    11701 acaatgaact gcttgtcgcc cgacgaggcc gtcgcgtgcc cggccaccag cagcgcgggg
    11761 ccgggggtca tcccgggggt caggctggag aagaggccgg cgaccgtgcc ggtcagcgcg
    11821 ccccgcgcca ggtcgtgggg ctcggcgtcc acgctgcccg cgaagtgctg gggcgggatc
    11881 gtcgcccgcg tgatgacggt catgatctgg ctgggcagcg cgaacaagcc catgaagacg
    11941 ggcatcagcg actggaacgc gctctcgacc gggatgatcg tgttgtagaa gacgaacatc
    12001 cccagcacgg ccgccgcgac aaaggtgaag aagcccatgc ccagctgcac ccagccgtcc
    12061 gccaggcgct gccgcacggt cttcccgacg ccgtggtcct tgggccattc gccgatcagg
    12121 atgaacgccg aaaccaggcc gaggatccag tagacgtgtg gccggatcag ttcgcgcacc
    12181 aggccgatcg gcttggtcag gaaggggaac agcccggcga tcagcaccag ggcgatcagc
    12241 gagccgaagc cggccaggat caccgcctcg tacccgcgcc cctcgtacag gaaccgctcg
    12301 tgcggcatca tcatggaacg gaagctgtcg tcgcacggct ggaagaactg gctcgagacc
    12361 gtgaagagca ccgagaatcc gaccaccatg cccaggatga agctggtggt cagcagggga
    12421 tccagcgcgg cgaagaactc gacgaacgcg aaggccagca tcatcgtgaa ggcgatcacg
    12481 ttgtagatgt gcaggccggg aatgaacgaa aagaccgcgg ccacggccac gcccaggagg
    12541 gccgtcggaa tgcacatcag gaccaggttg agttccatga atcagtctcc gcccgccgcg
    12601 gccacgggct cgatggcccg gggattgccc cgggccgtca tgatctccca gtagcccatc
    12661 ttttcgaagt acacaaagtg cccgcgtacc gccaccttct gcccgaccgt cagtgaccgc
    12721 ccgtcgaatc cgaacaccat gaccgtgttg gtctggccgg gctcgcggat gcggacgatc
    12781 gggtgcgtcg cggcgacgtc ggccacctcg gccgccgcca cgacgatcca gcggttgctg
    12841 aagcggccgg gctcggcgag cagttcagcg atcgtggcag gcatgtcctc ccgggcctcc
    12901 ggcatgctcc cggccggccg ggacttgatc ttcagcaggc tcgcgctgtt gacgctgatg
    12961 aatcggtacg acgagcctgc gaagagcgag ccggcgacct cgatcacgtc cccgcggcgc
    13021 ggcggatcct tcagcgctac gaaatcgagg gcggcctcgc cttccagccg gacggacagg
    13081 cgctggcggg ccggcgcgtc ggccgtcgcc aggtcctcga ggtcaaggcg cacgaggggc
    13141 gcgttgtacc ggtcctgcgt gacgttgacg ttgaccaccc gcccggctac gcggacgcgc
    13201 tcgaaattca tctcggcggt caggtcgccg agggccgtca ggcggggccg gtattgcagc
    13261 gccgcggtga agtgaatcgc ggcgatcacg aggagcagca aggcgcctcg cccgatccag
    13321 gccgtcgaga tgcgggtccg gagcgaggcc gcgcagaagc ggcagacccc tgactcggcg
    13381 gggaggggac gaccgcagct cgggcagagc aggggttcag ggcccggcat tgtccgccct
    13441 ccattgcgcc tcgtccgccg gctggatgtc ctccggcccg gccagcgcca gctgccaggc
    13501 ggattgcttc tggctccggt accgctgcag ggcgccgtag cacgtgatga agctcccctc
    13561 gcgcatctgt tccagttggg cgcctgcctg ctccgcgcgc cgggccttga cgacatcctg
    13621 ggagaggttc accgcgagcc ggttgccgcc ctcgtcctcg atcagccaga ccatgtcgta
    13681 ggagctgggg atgcgggact ggatgcggcc ggtgaccttc aggcgcgtgc cttcctcgag
    13741 tccctcgcgg aacgcccgca cgaccctggc gatcggcgtg gcctcgccgg gtttttcgcg
    13801 gacgatgtcg aggtcttcca cggcgctcaa gatcatgatc tggcgccggg ccttgtactg
    13861 gtagttgccg atcagcgaga cgcggtcgcc gaagaccggg atcttgtccg ccttcagcag
    13921 ttcctccgtc gtgtcgtcgt agcagcggat gcgcatcagg ccggtgccgt cgtcgacttc
    13981 gaactcgagg ctgctgttgc gccccttggc gtccgcggga tagtagcgca gatcgccgct
    14041 gatgcgcccc tccacgcgga cctgcgcgaa attggtcttg cggcccaggc tggagatctt
    14101 cacggtcggc gagccgtaca cgcggcccat gtgcgagagc agcacgatgc cggcgatggt
    14161 caggaagggc gtgctgtact tcaggaggcg cacgatccgc cgcttgggat tgaattggcg
    14221 gcagaaggga caccgctccc atgagccgat ataccgcccg caggctcggc aggtggtctc
    14281 cgccggattc tggctcatca ccagccggct aatctcgggg tccagtttga tgagccgcat
    14341 tctacacgcg ttgcggggaa aggacaaggg atatccgaac gagggcctga ccaagttgcg
    14401 cggcccgggg aatttccaca ctttgcaaag tgtggaaatt ttttcgggcg gaccgcggca
    14461 acgaggcgaa cggcatggca acgggggccg agttatgcta gagatggcct cgcccgcgat
    14521 ggcgcggaaa gaaccatggg ctggtggaaa caacaacgta gaagactgga agtcgggctc
    14581 gcgcgattgg cgatgggcgt tgtcaaccgg ctgccgcggc ccgttgttct cggcctggcc
    14641 cgatttctcg gcgcggccgg ctacctgctg gcccgctccc tgcggcgcat cggcctcgcg
    14701 aacctggacc tggcctacgg cgaggcgctg accggccgcg aaaagcggcg gcttctcaag
    14761 cggtccttcc agtcgttcgc cctggtcacg ctcgatgtct tctggttctc acgccgcacg
    14821 gagcagcgca tccggcgctg ggtatcgttc gagcggccgc tctccgaggt gctgggccgc
    14881 ggcgccaacg tctgcctgac cggccacctc ggcaactggg aagtcctggg cctggcctgc
    14941 gccgtcgagg gctatcccct ggccagcgtc gccatgccgc tgaagaatcc cgacgtggac
    15001 gtcctcttca accggcttcg acagtccacc ggccagacca tcatcccccg cgagggcgcg
    15061 gtccgcgcgc tcctgcggct cctgcgcgag ggacgcaacg cggcgctgct gctggaccag
    15121 aacacccggc cggagcacgg cgggctgttc gtggatttct tcgggcggcc ggcgaccgtg
    15181 tcgggcgccg ccgtcttcct ggccgcccgc gcgggctgcg gcatcgtgtc gggcttctgt
    15241 ctgcccgacg ggcgcggcgg ctaccgcgtg cgcctgcccg acgggctggg cgtgccgccg
    15301 ccgaagcccg acgatacccg ggtccgcg
//
LOCUS       DAOOTK010000076        15316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2613_length_15316_cov_4.587773, whole genome shotgun sequence.
ACCESSION   DAOOTK010000076 DAOOTK010000000
VERSION     DAOOTK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15316
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_15316_cov_4.587773"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1239
                     /locus_tag="PK634_09240"
     CDS             328..1239
                     /locus_tag="PK634_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97959.1"
                     /translation="MRLATAGLAVLLAAGKLRAAETYLTPSIEAQAGFEQNRWTESGS
                     GQGSAFWLAAPGVEWLRFGQKTEAALGAEYRVTSYFEEGFEYREEPSGYGRWRYFDGP
                     YEAGLTAGGGQYRDRALPDDDCAYWYARPYASRTLARRPAEWTLGGTIRQTFYDTSVY
                     TSATDRTDLQLEVRPGLRWHFSTRLTLWAEGYAEWNTSDAPEAEYSGFGAAAGGQYQA
                     SARWTLGAWAETGTRVYDVEVDGTEWRDTPLRAGVWTTCRLRPWLELLAAVDGESFSS
                     TLDGNDYSWWRAAAGIRLVFEHVMGAR"
     gene            1295..1492
                     /locus_tag="PK634_09245"
     CDS             1295..1492
                     /locus_tag="PK634_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97960.1"
                     /translation="MRWQKTKEWISTSWARAWFALTPEERKFIGGILLIFVIGLVARY
                     LYLKSEKPEPFTPAGLERTAP"
     gene            1489..1917
                     /locus_tag="PK634_09250"
     CDS             1489..1917
                     /locus_tag="PK634_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012123240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOW97961.1"
                     /translation="MNESCIFCKIIRGEIPSTRVYEDEHTLAFLDIGPLVKGHTLVIP
                     KQHFDSLRDADPEAAAQVMVTAQKIARAQLNGLGADGVNIHQANGRVAGQVVPHLHVH
                     VVPRFKDDGYHWNWKAGKYAGADESNGMAEKIRQGLKQTA"
     gene            1914..2336
                     /locus_tag="PK634_09255"
     CDS             1914..2336
                     /locus_tag="PK634_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020152853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97962.1"
                     /translation="MKKPSYEESLQQVAREDPRYDVEAYEFLREALDFTIKLLSKPTE
                     GPARHVTGGELLEGIRQYALQEFGPMALTVLNRWGVRRCEDIGEMVFNLVEKGILGKT
                     ERDKKEDFSGGYDFKQAFREPFLAGGKADLPAPPGRDN"
     gene            2340..4982
                     /locus_tag="PK634_09260"
     CDS             2340..4982
                     /locus_tag="PK634_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOW97963.1"
                     /translation="MATRWLITFLLALLAARPAARAAEPGGNPVETLRRAHEGLRRTV
                     LNNGMALLVKEDHSAPVVAIQVWVGSGSIHEQEWLGAGLSHFVEHMIFKGTPTRSPGE
                     ISRQISDAGGDINAYTGFDRTVFHVTLPARNWTVGLDVLADAVMNASFPEEEWARERE
                     VILREFAMHRDNPDREVGQMLWANAFRAHPYRIPVIGYEDVFTATTRDDLAAYFRRNY
                     TPDNMIVSIVGDVPADEVEQRVKETFAGFARRARAPLLLPAEPVQISPRFERRTGPYE
                     VTRVRMAWPTVPMNHPDAAALDVLAALMGQGRSSRLTSELVEKRKLAFDLSAWSYTPL
                     DIGLFGIGGTCDPARETDLLNAVEEDVAGWAAMTVPPEDLAKARRQVLVGELSSLEDM
                     AGQAASFASGEFYAADPRFSERYLQGVERVDAASLRDVARRYLSPEKRTLVILAPAGT
                     GATVQAVEPPRPGSDVVKIELPGGIPLIVREDHRLPFVNVAIVLRGGLLSEDADRNGI
                     TPLMAELLTRGTASRSSEEIAQQVEQLGGSLSAFSGWNSFGLQARGLSQDAAVFLDLA
                     AECLLQPSFDPAEIDKQRDLQKAAIRRQREQPMYRAQVALQEALFPGHPYRLPELGTE
                     ESVDRIRREDLVAHLQKLAVSGNLALSVFGDIDPEEARRLVEKAFAGVPRGQAPEPPR
                     VASAPTLPSRSESREPREQAILLVGYPGVDVRDPRCDALAVVRDTLSGLSSDLGVEVR
                     DRRGLAYFVGAFSREGLQPGLFALYAGAKEDTVQQVEELMAAQVERLGREGPRPEELQ
                     RAVEQLVAGHQMSLQDNDGLAQLCALHELYGLGYRYAFTAEERWRALTVEQVRDAAAS
                     LLKPDRQAVSIVLPETSKTHGEKK"
     gene            4979..5359
                     /locus_tag="PK634_09265"
     CDS             4979..5359
                     /locus_tag="PK634_09265"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOW97964.1"
                     /translation="MKEEARDAEMHKSLFVQLVMMLATSASQAMGKLVNPATKKTEVN
                     VEAAEATIDLLDMIEAKTKGNLDRDEERLLKDTLMSLKLTFVETRNAAPAAAPAESAE
                     PAAAPGSKVEGGPAGQEPRFHKSY"
     gene            complement(5366..6385)
                     /locus_tag="PK634_09270"
     CDS             complement(5366..6385)
                     /locus_tag="PK634_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOW97965.1"
                     /translation="MKYPVVLVAVALAVSRDAGAAGFRRMSGEVPIQPPRIVRYDAGA
                     TGAVGDHVRLASYNIENFTDGVDDKEPRIPAHAEIQAKAAAALVAEIDPDILVIEEIE
                     NETSLELLNRALPAPYPVAAVTRIKSAPDWPEKLNLAVLSRLPIRGLRELDFGYLDGI
                     HSPPRGLLSFYIELEQGRNLLVYAVHLKSNFSGDRSVNYAQRVNALRLLREDAESFRR
                     TYNDREWDVLMLGDTNTDPDMPEFARDPTLRPLGDWVDLWRGHPMSERETIPTRYGDP
                     AKIFPPAAFDRFVVTPGLTEPPWVAGEPHVLQKGVNTADITALPGVDPHHASDHYPVY
                     LDIRR"
     gene            complement(6401..>6657)
                     /locus_tag="PK634_09275"
     CDS             complement(6401..>6657)
                     /locus_tag="PK634_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOW97966.1"
                     /translation="KLVRTRLRAACVCVEGDYLVRNGYFGLGREHPALAQRVGDYVLI
                     ARAGYAFNTTPPGMPSKFKRGNHGGMSAEEMRVPLFTVIA"
     assembly_gap    6658..6667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6668..7604)
                     /locus_tag="PK634_09280"
     CDS             complement(<6668..7604)
                     /locus_tag="PK634_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOW97967.1"
                     /translation="MSLIRTGPLTGLYAPEYRGNSIVNLLASLLRARGGRSPSPGLRG
                     FPARAFREARRAVYLVLDGLGEHQLRWYLGRARQSPFFGRQPRSVLTTVFPATTAAAV
                     TTFATGAPPREHGVLGWHLHLGDLGMVSTILIGVTRTGMPMMGPDFPLKEYMRLPSHL
                     ETVPGRRTLISYGDIPDSRYSRAGLRWHETVACTTLGGLVRHVAAFARRPGRGLAYAY
                     WPGLDSLCHEWGCFHAKSYAHMAALDRAMAGLVRRLRGTDTALAITADHGLVDTPPGT
                     CIELREVPGFYDCLAMLPSGDAREVSCFVRPAKLQA"
     gene            7715..8662
                     /locus_tag="PK634_09285"
     CDS             7715..8662
                     /locus_tag="PK634_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOW97968.1"
                     /translation="MSRCLVTGAAGFIGSHLCDALLDQGHDVVGLDAFIPYYPRAVKE
                     ANLAGARRHPRFTFHELDLRTADLAGAVSGCEVVFHEAAMPGLMRSWSDLDLYASCNL
                     VGTQRLLEAARQARVAHVIHVSTSSVYGREATGPEDSALQPCSPYGITKLGAEHLCRT
                     YAGHFGVPVTILRYFSVYGPRQRPDMAYHILIRCLLRNEPFTLYGAGDQTRSNTYAAD
                     CVQATLLAMAQRERALGGTFNVGGGQVVSLNEVIALLEELTGRRLDIRRKPARPGDQK
                     HTAADITRIASVLSYQPSTPVREGLQAQLAWERQILSAT"
     gene            8932..9486
                     /locus_tag="PK634_09290"
     CDS             8932..9486
                     /locus_tag="PK634_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97969.1"
                     /translation="MYFYQHKMRDVGCCLFLLALTSLVSQGDVINQYGATGNMGGVFN
                     QWPSVWTPILSLNDGDNAKTECLDFVGDNSNPGAYMANENGYLFFRMRVDEGTITSGT
                     YADNIWIYIDRLGYNYGGNADGQPDYALAWDTQKSHGLEFQTWSANGNSWNIEAMNDI
                     DATPGSKSCSPRYQHKRRRFCAVG"
     gene            complement(9948..11027)
                     /gene="aroC"
                     /locus_tag="PK634_09295"
     CDS             complement(9948..11027)
                     /gene="aroC"
                     /locus_tag="PK634_09295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOW97970.1"
                     /translation="MSSTFGTVFRVTTFGESHGRGVGAVVDGCPPRLALAETDIQPQL
                     DRRRPGQSGLSTPREESDKVSILSGVENGLTLGTPIGLFVPNRDQQPGDYAAMSRIPR
                     PSHADWTYQVKYGIRASSGGGRSSARETIGRVAAGAIAEKLLKQHFGVEIVAWVSAVG
                     AVEAPDLAGREITRPDVDAHAVRCPDAEAAQRMEAAIRQAREARDSTGGVITAICRGV
                     PAGWGEPVFDKLEARLAQAMLSIPATKGFEIGSGFAGSRMLGSEHNDPFVMRKGRLGT
                     ATNRSGGVQGGISNGEPIVFRVAFKPPATIGKAQATVDFEGHAVELEATGRHDPCVVP
                     RAVPIVEAMAALVLADAALAQCARS"
     gene            11154..11603
                     /gene="gspG"
                     /locus_tag="PK634_09300"
     CDS             11154..11603
                     /gene="gspG"
                     /locus_tag="PK634_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOW97971.1"
                     /translation="MKYQSERRTAGFTLIEILVVVLIITILAGIVGVNILHKPGEARV
                     SAARMQLQQLKTAVQLYRAEQGAVPTQEQGLEALVSKTTREPIPQNYPEGGYLDSRKV
                     PLDPWSHDYIYLAPGRRNESFEILSYGSDGEPGGGGEAGDLTSSDLQ"
     gene            11600..11944
                     /locus_tag="PK634_09305"
     CDS             11600..11944
                     /locus_tag="PK634_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOW97972.1"
                     /translation="MRRGPRRRRAGFTFLEAIIALAVAALLVTVSASSLASVFRADRT
                     AERLFEATLLLRAAAAGEYLGAPLEDLPEAWPAGWQLQAEQVETGKEPMRWKVLQVLS
                     DEIALASAFRQP"
     gene            complement(11987..13585)
                     /locus_tag="PK634_09310"
     CDS             complement(11987..13585)
                     /locus_tag="PK634_09310"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOW97973.1"
                     /translation="MKLVAWYRRQPIRRKIFIPFFGITLFSSTIFTIYGFKQNVKAIQ
                     DGIDKRLLIAASTMPQLLPADYFERVQHPGSITGDEHWNNTRRLDIFLRNIGGTYLYA
                     LLQESNRYYFVASADTGTPYWVQYTNPAPNIYEVQKDWKVNISTTPDPDYGLLRSVVM
                     GFKDSSGRRFIVGADIHAYEVEALKRRAFLNFFLMGAASFILAVIFSYAASYSITRPL
                     NRLSNFTCRLAEGEFSKDIRLDPALFPDGNRTKAETAILAYDFDRMQSNLAEHIEQLK
                     LTQSARERAESELRIAGQIQATFLPGPFDPAELGGRVQLQAVMKTAKQAGGDLFDYFP
                     LDADHLFFAVGDVSGKGMPAAMFMSAVVVLLRSAAKQWRDPAEILRRVNDDLAIRNES
                     CTFVTLFVGILDVRTGEVVFANGGHNPPRRRAADGPVAVVRTRSNIVVGAMDGRTFAR
                     EILTLQPGETLILYTDGITEAFNEADQLYGEARMDRRLASLPATASVADVLQGVVDDV
                     VAFSGAREQSDDITVLIMRYHPGP"
     gene            complement(13582..>15316)
                     /locus_tag="PK634_09315"
     CDS             complement(13582..>15316)
                     /locus_tag="PK634_09315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW97974.1"
                     /translation="ALITFQDHALVVDADAVDRIQHVKHYMNADYQAVLKELCQTITR
                     EGVIDPGTYSGVYKVFGDKLSALAYHDGLRGIFYPYNYQYSKSIYAEVVRTGKPYVGE
                     VVDIYGVWLNGVVPLVNSNGVTVGLLEVGADQSAQREANRTLFKSTLMDLAMVLFVLL
                     FVFFEIGFFSSHVLDRHDDTAGQRYDEGAVRFVSFMAIVGVFLSASFLPLYSKSLAPA
                     IGRIPFDVVIGLPMVIETLCGAVIALLYGHVRLRAGIKTDVVLAGLVITGGMVVTALS
                     NTFVRLIAGRVLVGMGMGLLMIAFRTYFLIEKDEGRKESGIIALTAGVIAGINVGSVS
                     GGMLAARVGMRPVFAIQAVLMVLAAVAALALIRNRRREPAGDRATGAPSPWAFFIDRA
                     VWSFFLFIFLPVTACGLFLGFLFPLFAESQGCSINEISLAFMLFGAASVYLGPAITRL
                     ATNLFGARRAMPIGALVMTAALLLFAALRSLAAAYAAIILFGLTESLLFNQGLSYYSS
                     LPRVRRFGADKAMGIYNLFESGGEALGPMAFGLAASFSLGIGISAIAAVLGSCALLFW
                     AVSPRGGGGRM"
BASE COUNT         2730 a         4898 c         5246 g         2432 t
ORIGIN      
        1 ttggcctgtc ccatattcaa accctggcgg aagccggttt tgattatatt gaactgtcca
       61 tggcggcggt gatgtccgtc gacgcggaga atttccggaa tgctgcggaa aagatttccc
      121 gctcgccgct tccgtgcatc gtggtcagtt cgttcctgcg tccggaggtt aaggccgtgg
      181 gaaacggata tgatgaaaat gtgcttgtcc aatatgtaag ggaggcagcg gccagggcgg
      241 atcggacaat cagggcaaca gcgggacggg cggcgactcg tccaagaact gaacggtccc
      301 tctgcaccgg tgaaaatctt ccccaccatg aggctcgcaa cagccggcct ggcggttttg
      361 ctcgccgccg ggaaactccg cgccgccgag acgtacctga ccccgagcat cgaggcccag
      421 gcaggcttcg aacagaatcg ctggactgaa tccggcagcg gccagggctc tgcgttctgg
      481 ctggctgcgc cgggagtgga atggctccgg ttcggccaga agacggaggc cgcactgggc
      541 gccgagtacc gcgtgaccag ttacttcgag gagggattcg aataccggga ggagccctcg
      601 ggctatggcc gctggcgcta tttcgacggc ccctacgagg ccggcctgac ggccgggggc
      661 ggccagtacc gggaccgcgc cctgcccgac gacgactgcg cgtactggta cgcgcggccg
      721 tatgcgagcc ggacgctggc gcgtcggcct gcggaatgga cgctgggggg aacgattcgg
      781 caaaccttct acgacacgtc ggtttacacg tcggccaccg accggaccga cctgcaactg
      841 gaggtgcggc ccggtctgcg ctggcacttt tccacgcggc tcacgctgtg ggcggagggg
      901 tatgccgagt ggaacacgtc cgacgcgccc gaggcggaat actcgggttt cggagcggcc
      961 gcgggcggcc agtatcaggc ctcggcccga tggaccctgg gggcctgggc ggaaacggga
     1021 acgcgggtgt acgacgtcga ggtggacggc accgaatggc gggacacgcc gcttcgcgcc
     1081 ggcgtgtgga ccacgtgccg gctccggcct tggctggaac tgctggccgc cgtggatggg
     1141 gaatcctttt ccagcacgct ggacggcaac gactacagct ggtggcgggc ggcggccggg
     1201 attcgcctgg tattcgaaca cgtcatgggc gcccgctgac gcagggtcca atccccccat
     1261 tgcccgcggg ccggagtcgg ggtacagtgg gaccatgcgc tggcagaaga cgaaggagtg
     1321 gatcagcacg agctgggctc gagcgtggtt cgcgctgacg ccggaggagc ggaagttcat
     1381 cggcggcatc ctgctgatct tcgtcattgg gctggtcgcg cgctacctct acctgaaatc
     1441 ggaaaagccc gagccgttca caccggccgg actggaaagg acggccccat gaacgaatcc
     1501 tgcatcttct gtaaaatcat ccgcggcgag atcccgtcca cgcgcgtgta cgaggacgag
     1561 cacacgctgg ccttcctgga catcgggccg ctggtcaagg ggcatacgct ggtcatcccg
     1621 aagcagcact tcgactcgct ccgcgacgcc gatccggagg ccgccgcgca ggtgatggtc
     1681 acggcgcaga agatcgcgcg ggcgcagttg aacgggctgg gcgccgacgg cgtcaatatc
     1741 caccaggcca acggccgggt ggcgggccag gtggtccccc acctgcacgt gcacgttgtg
     1801 ccgcggttca aggacgacgg ctaccactgg aactggaagg ccgggaagta cgccggggcg
     1861 gacgagtcca acggcatggc cgagaagatc cggcagggat tgaagcagac ggcatgaaga
     1921 aaccaagcta cgaggaaagc ctgcagcagg tcgcgcgcga ggatccgcgg tacgacgtgg
     1981 aggcctacga gttcctgcgc gaggcgctgg atttcaccat caagctgctc tccaagccca
     2041 cggaaggccc ggcgcggcac gtcaccggag gggagttgct cgaaggcatc cgccagtacg
     2101 cgctccaaga attcgggccg atggcgctga ccgtgctgaa ccgctggggc gtccggcgct
     2161 gcgaggatat cggagaaatg gtgttcaatc tggtcgagaa gggcatcctc ggcaagaccg
     2221 agcgggacaa gaaggaagat ttttcgggcg gctatgactt caagcaggcc ttccgggagc
     2281 cgttcctggc cggcggcaag gcggacctgc ccgccccgcc cggaagggat aactgaaata
     2341 tggccacgcg atggctgatt acctttctgc tggcgctcct ggccgcgcgg ccggcggcga
     2401 gggcggcgga gccgggtggg aatcccgtcg agacgttgcg cagggcgcac gaggggctcc
     2461 ggcgcacggt gctgaacaac gggatggcgc tgctggtcaa ggaggaccac agcgcgccgg
     2521 tcgtggcgat ccaggtctgg gtcggcagcg ggtccatcca cgagcaggaa tggctgggcg
     2581 cgggactgtc ccacttcgtc gaacacatga tcttcaaggg gacccccacc cgctcgccgg
     2641 gcgagatcag ccggcagatc agcgacgcgg gcggggacat caacgcgtac accggcttcg
     2701 accggacggt cttccacgtg acgctgcccg cgaggaactg gaccgtcggc ctcgatgtgc
     2761 tcgcggacgc cgtgatgaac gcctcgttcc cggaggagga atgggcccgg gagcgggagg
     2821 tgatcctgcg ggagttcgcg atgcaccgcg acaatcccga ccgcgaggtc ggccagatgc
     2881 tctgggccaa tgccttccgg gcacatccgt accgcatccc ggtgatcggc tacgaggacg
     2941 tgttcaccgc gacgacccgg gatgacctcg cggcctattt ccgccggaac tacacgccgg
     3001 acaacatgat cgtctccatc gtcggcgacg tgccggccga cgaagtggag cagcgcgtaa
     3061 aagaaacgtt tgccggcttc gcccgccggg cccgggcccc tctgcttctg cccgcggagc
     3121 cggtccagat ttccccgcgc ttcgaacgaa gaacgggacc gtacgaggtg acccgggtgc
     3181 gcatggcctg gcccaccgtc ccgatgaatc atccggatgc cgccgcgctc gacgtcctgg
     3241 ccgccctgat gggacaggga cgcagttcgc gcctgacgtc cgaactcgtg gaaaagcgca
     3301 agctggcttt cgacctgtcg gcctggtcat acacgccgct cgatatcggc ctgttcggga
     3361 tcggtggcac ctgcgatccg gcccgcgaga ccgacctgct gaacgccgtc gaggaagacg
     3421 tggccggctg ggccgccatg accgttccgc ccgaagatct ggccaaggcc cggcggcagg
     3481 tcctcgtcgg cgaactgtcg tcgctggagg acatggccgg gcaggcggcc agcttcgcct
     3541 cgggtgagtt ctacgccgcc gatccgcgct tcagcgaacg gtatctccag ggcgtagagc
     3601 gagtggacgc ggcctccttg cgtgacgtgg cccggcgcta cctgtcgccc gagaagagaa
     3661 ccctggtcat cctggccccg gccggcaccg gcgccacggt ccaggccgtc gagccgccgc
     3721 ggcccggatc cgacgtcgtt aaaatcgagt tgcccggcgg tatcccgctg atcgttcggg
     3781 aggaccaccg cctgccgttc gtgaacgtcg ccatcgtgct tcgcggcggg ctgttgagcg
     3841 aggatgcgga tcgcaacggc atcacgccgc tcatggcgga actgctgacc cggggcaccg
     3901 cgtcgagatc gtccgaggaa atcgcgcagc aggtggaaca gctgggcggc tcgctctcgg
     3961 ccttctcggg ctggaacagc ttcggcctgc aggcgcgcgg cttgagccag gatgccgccg
     4021 tgttcctgga cctcgcggcg gagtgcctgc tccagccctc cttcgatccg gctgaaatcg
     4081 acaagcagcg cgacctgcag aaggccgcca tccggcgtca gcgcgagcaa cccatgtacc
     4141 gggcgcaggt ggccctgcag gaagcgctgt ttccgggcca cccctaccgg ctccccgagc
     4201 tgggcacgga ggaaagcgtg gaccgcatcc ggcgcgagga tctcgttgcc caccttcaga
     4261 agctggccgt ctccggcaac ctggccctct ccgtgttcgg cgatatcgac ccagaggagg
     4321 cccggcggct cgtggagaag gcgtttgcgg gcgttccccg aggccaagct cccgaaccgc
     4381 cgcgcgtcgc ctctgctccg acgctgccat cgcgctcgga atcgcgtgaa ccccgggagc
     4441 aggccatcct gctggtgggc tacccgggcg tggatgtccg cgatccgcgg tgcgacgccc
     4501 tggccgtggt ccgcgacacc ttgagcgggc tctcatccga cctcggcgtg gaagtgcggg
     4561 accggcgcgg cctggcctat ttcgtcggcg ccttctcgcg cgaagggctg cagcccgggc
     4621 tgttcgcgct gtacgccggg gccaaggaag acaccgtcca gcaagtcgag gaactgatgg
     4681 ccgcgcaggt cgaacggctg ggacgggaag gcccgaggcc cgaggaactg cagcgcgcgg
     4741 tcgagcagct ggtcgccggc caccagatga gcctgcagga taacgacggg ctggcccagc
     4801 tgtgcgcgct gcatgagcta tacggcctcg gataccggta tgccttcacg gcggaggaac
     4861 gctggcgcgc cctgaccgtg gagcaggtgc gcgatgccgc cgcctcgctg ttgaagcccg
     4921 accggcaggc cgtttcgatc gtgctgcccg aaacctccaa aacccacgga gagaagaaat
     4981 gaaagaagaa gcgcgcgacg ccgagatgca caagtccctg ttcgtccagc ttgtcatgat
     5041 gctggccacc tcggccagcc aggcgatggg caagctcgtt aacccggcca cgaaaaaaac
     5101 cgaggtgaac gtggaggccg ccgaggccac catcgacctg ctggacatga tcgaggccaa
     5161 gaccaagggc aacctcgacc gggacgagga gcgcctgctg aaggacacgc tcatgtccct
     5221 caagttgacc ttcgtggaaa cccggaatgc ggcgccggcc gctgcgcccg ccgaatcggc
     5281 tgaacccgcc gcggcgccgg gatcaaaggt ggaaggcggc ccggccggcc aggagccgcg
     5341 gttccacaag tcgtattgac ccggcttacc ggcggatgtc gagatagacc gggtaatggt
     5401 ccgacgcgtg gtgggggtcc acgccgggca gggcggtgat atccgccgtg ttcacgccct
     5461 tctgcagcac gtgcggttcg cccgccaccc agggcggttc ggtcaggccc ggcgtgacga
     5521 cgaagcggtc aaaagcggcc ggagggaata ttttcgccgg gtcgccatac cgggtgggaa
     5581 tcgtttcccg ctcgctcatg ggatgcccgc gccagagatc cacccagtcg cccaggggcc
     5641 ggagcgtcgg atcccgcgca aactccggca tgtccgggtc ggtattggtg tcccccagca
     5701 tcagcacgtc ccactcgcga tcgttgtagg tccggcggaa gctctcggca tcctcccgca
     5761 gcaggcgcag cgcgttgacc cgctgggcgt agttgactga gcgatcgccg ctgaagttgg
     5821 acttcaagtg caccgcatac accagcaggt tccggccctg ttccaactcg atgtaaaagc
     5881 tcaacaggcc gcgcggggga ctgtggatgc cgtccagata gccgaaatcc agctcgcgca
     5941 agccccggat cggcaggcgc gagaggaccg ccaggttcag cttctcgggc cagtccgggg
     6001 cgctcttgat gcgcgtgacg gcggccaccg ggtacggcgc cggcagcgcg cggttgagca
     6061 gttcaagcga ggtctcgttc tcgatctcct cgatgaccag gatatcggga tcgatctcgg
     6121 ccacgagggc ggcggcggcc ttcgcctgga tctccgcgtg cgccgggatc cggggctcct
     6181 tgtcgtccac gccgtccgtg aaattctcga tgttgtaact ggcgagccgc acgtggtcgc
     6241 ccacggcgcc cgtggccccg gcgtcgtacc gaacgatgcg gggcggctgg atcggaacct
     6301 cgccggacat gcgtcggaag ccggccgcac cggcgtcccg gctcacagcg agggccacgg
     6361 caaccaatac gacggggtat ttcatgacga gtcctttgga ttatgcgatg acggtgaaca
     6421 ggggcacgcg catttcttcg gcggacatgc cgccgtggtt gccccgcttg aacttcgagg
     6481 gcatccccgg cggcgtggtg ttgaaggcat agccggcccg cgcgatcaac acgtagtcgc
     6541 ccacacgctg ggccagcgcc ggatgctccc ggccgagtcc gaaatatccg ttccgcacca
     6601 ggtagtcgcc ctccacgcac acgcacgccg cgcgcaggcg ggtccggacc agtttcannn
     6661 nnnnnnnagg cctgaagttt cgccggccgg acaaaacagg acacctcgcg ggcgtcgccc
     6721 gacggcagca tggccaggca gtcgtagaaa cccggcacct cgcgcagttc gatgcacgtg
     6781 cccggcgggg tgtccaccag gccatgatcc gcggtgatgg ccagggcggt gtccgtgccg
     6841 cgcagccgcc gaaccaggcc ggccatcgcc cggtcgagag cggccatgtg cgcgtacgac
     6901 ttcgcgtgga agcaccccca ctcatggcaa agggaatcga ggccgggcca gtaggcgtag
     6961 gcgaggcccc gacccggccg tcgggcaaag gcggccacgt gccggacaag gcctcccagc
     7021 gtcgtgcagg ccacggtctc gtgccagcgc aggccggcgc gggagtagcg gctgtccgga
     7081 atgtcgccgt aggaaatcag cgtgcgccgg ccgggcacgg tttccaggtg ggagggcaga
     7141 cgcatgtatt ccttcagcgg gaaatcgggc cccatcatcg gcatgcccgt gcgcgtgacc
     7201 ccgatgagga tggtgctgac catgcccagg tcgccgagat gcaggtgcca gcccagcacg
     7261 ccgtgctcgc gcggcggggc cccggtcgcg aacgtcgtca ccgcggccgc cgtggtggcc
     7321 gggaaaaccg tcgtgaggac cgaccggggc tgccgcccga aaaaggggct ctgccgggcg
     7381 cggccgaggt accagcgcaa ttgatgttcc cccagcccgt cgagcaccag gtagaccgcg
     7441 cgcctcgcct cgcggaacgc gcgggcggga aacccgcgca accccgggga aggcgatcgg
     7501 ccgccgcgcg cccgcagcag gctggccagc aggttcacga tgctgttgcc gcggtattcc
     7561 ggcgcgtaca acccggtcag cggacctgtc cggatcaagg acatggggcg tactataggc
     7621 ccgcgggacg gctttctcaa gggccggctt tttcggctgg ttttgccgtc cgcgttgttg
     7681 tttcatggcg ccggaaggaa acaccgaatt ggccatgagt cgatgcctgg taaccggcgc
     7741 cgcgggattc atcggatcgc acctgtgcga cgccctgctc gatcagggcc acgacgtggt
     7801 cggcctggac gccttcatcc cctactatcc ccgcgccgtc aaggaggcca acctggccgg
     7861 cgcgcgccgg catccgcgtt tcacctttca cgagttggac ctgcgcacgg ccgacctggc
     7921 cggcgccgtg tccggttgcg aggtggtctt ccacgaagcg gcgatgcccg ggctcatgag
     7981 gagctggagc gacctggatc tctacgcgtc gtgcaacctg gtcggcaccc agcggctgct
     8041 cgaggccgcg cgccaggctc gcgtggcgca cgtgatccac gtctccacgt ccagcgtgta
     8101 cgggcgggaa gccacgggcc ccgaggattc cgcgctgcag ccctgctcgc cgtacggcat
     8161 caccaagctc ggggccgagc acctgtgccg cacctacgcc gggcacttcg gcgtgccggt
     8221 gaccatcctg cgctatttct cggtgtacgg gccgcggcag cgcccggaca tggcgtatca
     8281 catcctcatc cgctgcctgt tgcggaacga gccattcacc ctgtacggcg cgggggacca
     8341 gacccgcagc aacacctacg cggccgactg cgtgcaggcc accctgctcg ccatggcgca
     8401 gcgggagcgg gcgctgggcg gaactttcaa cgtgggcggc ggccaggtgg tctcgctcaa
     8461 cgaggtgatc gccctgctgg aggaactcac cggccgccgg ctggatatcc ggaggaagcc
     8521 cgcgcggccc ggcgaccaga aacacacggc ggcggacatc acccgcatcg cgtccgtcct
     8581 cagctaccag ccctcgacgc ccgtgcggga aggcctgcag gcgcaactgg cctgggaacg
     8641 tcagatcctg tcggccacgt gatggctgca ggaatcacag cactcgatat acgttaagcc
     8701 ttcgaaacgc agacgggcgt tacttagcga tcaagttcca acctgataaa ccactcgacg
     8761 acagaacggc ttgagagcaa ataacgcccc gttaatatca agagcggcat ctgaatcaag
     8821 ccggcgtcag tcggggagga aatggttgtc ggcaattcaa aatacttgtc actactttga
     8881 cgcgaaatgg acttatgggt agattttcgc ttcattattg gagaaatcta gatgtatttt
     8941 tatcagcata aaatgaggga tgtcggttgt tgtctttttc tattggccct caccagtctt
     9001 gtctcgcaag gcgatgtcat caatcaatat ggcgccaccg gcaacatggg tggcgtattc
     9061 aatcagtggc cctccgtatg gacccccatc ttgagcctca atgacggaga taatgctaaa
     9121 accgagtgtc tcgatttcgt aggcgacaac agcaatccgg gcgcgtacat ggccaatgaa
     9181 aatgggtatc ttttcttccg aatgcgcgtg gatgagggaa cgatcaccag cggcacctat
     9241 gccgacaaca tttggattta tatcgatcgg cttggttata actacggcgg caatgccgac
     9301 ggacagcccg actacgcgct cgcgtgggac acacagaaat cgcatggatt ggagtttcag
     9361 acctggtctg ccaacggtaa cagctggaac atagaagcga tgaatgatat tgacgcaact
     9421 ccaggctcca aaagttgttc cccccgatat cagcacaaac ggcgacggtt ttgtgcggtc
     9481 ggatgataat cagtcaaccg tcaacttcgg cgcaacatcg ttcatcgatt gggcggttga
     9541 atggagctac ctggaaagca acagcactct agcgccgggg cagaactaga agattcagtt
     9601 cggcagtcga tatcatggta acgatcacaa taatccggac gaagacgtcg ccggcggttt
     9661 ttcacccagc agttccgtca ccaattcctg gagcgccttg gtcaattccg ccgtccccga
     9721 gccacagtac ggccttattg cgggattcct tggaatcgct tacgtcatcc attcctggct
     9781 gacggtgcgg ccgaggaata aaaggactgg acatctgatc ccctggttga gccgctgact
     9841 tgctgatggc gacaaccacg cgccgctccg cagcaatgga ccggcccgcc gaacgtcggc
     9901 ccgttaatcg tttcccgccc ctgcctttgt ttttccgggc ggcggaatca ggaacgcgcg
     9961 cactgagcta aagcggcatc cgcgagcacg agggccgcca tggcctcgac gatcggcacc
    10021 gcgcgcggga ccacgcaggg atcgtgccgg cccgtggctt ccaactcgac ggcatgcccc
    10081 tcgaaatcca cggtggcctg cgccttcccg atcgtcgcgg gcggcttgaa ggccacgcgg
    10141 aagacgatgg gctcgccgtt ggagatcccg ccctgcacgc cgccgctgcg gttggtggcg
    10201 gtcccgagcc ggcctttccg catgacgaac ggatcgttgt gctcggagcc gagcatccgg
    10261 ctgcccgcga accccgagcc gatctcgaat cccttggtcg ccgggatgga caacatggcc
    10321 tgcgccaggc gagcctccag cttgtcgaac acgggctcgc cccagcccgc cgggacgccg
    10381 cggcatatcg ccgtgatcac accgcccgtc gaatcgcggg cttcgcgggc ctggcggatg
    10441 gcggcctcca tgcgctgcgc cgcctccgcg tccgggcacc ggaccgcgtg cgcatcaacg
    10501 tccggccggg tgatctccct gcccgccagg tccggggcct ctaccgcgcc caccgcgctg
    10561 acccacgcga cgatctccac gccgaagtgc tgcttcagca gtttctccgc gatcgccccg
    10621 gcggcgacgc ggccgatcgt ctcgcgcgcg ctggaccgcc ccccgccgct ggacgcgcgg
    10681 atgccgtact tgacctggta tgtccagtcg gcatgcgagg gccgggggat gcgggacatc
    10741 gcggcatagt cgccgggctg ctggtcgcgg ttcgggacaa acaggccgat gggcgtgccc
    10801 agcgtgagcc cattctccac accggagagg atgctgacct tgtcgctttc ctcgcggggg
    10861 gtggatagcc cgctctgccc cggccggcga cggtcgagtt gcggctggat gtccgtctcg
    10921 gccagcgcca gccgcggcgg gcagccgtcc accaccgccc cgaccccgcg gccgtgcgac
    10981 tcgccgaacg tcgtgaccct gaacaccgtg ccgaacgtgc tggacatggc agctcccttc
    11041 cttcgcgccg aagcatagga cggcgccgga ccggatgcaa ccctcgcttc gccgaccgcc
    11101 gcgcttgacg aaccccggga aaagaccgat cttgtgccgc ggggatgcag accatgaaat
    11161 accaatccga gcggcgaacg gcgggcttca cgctgatcga aatcctggtc gtcgtgctga
    11221 tcatcacgat cctggccggg atcgtcgggg tgaacatcct ccacaagccc ggcgaggccc
    11281 gggtatcggc cgcgcgcatg cagctccagc agctgaaaac cgcggtccag ctctaccgcg
    11341 ccgagcaggg cgccgtgccc acccaggaac aggggctgga agcgctggtg tccaagacca
    11401 cgcgcgagcc catcccgcag aattacccgg agggcggcta cctggactcc cgcaaggtgc
    11461 cgctggatcc gtggagccac gattacatct acctggcgcc cggccggagg aacgagtcgt
    11521 tcgagatcct cagctacggg agcgacggcg agcccggcgg cggcggcgag gccggggacc
    11581 tgaccagttc ggacctccaa tgaggcgcgg gccacggcgc cgacgggcgg gcttcacctt
    11641 cctggaggcg atcatcgccc tcgccgtggc ggcccttctc gttacggtca gcgccagctc
    11701 gctggccagc gtttttcgag ccgaccgtac ggccgagcgc cttttcgagg cgacgctcct
    11761 gctccgcgcc gccgccgcgg gcgagtacct gggggctccg ctggaggacc tgccggaagc
    11821 ctggcccgcc ggctggcaac tgcaggccga gcaggtggaa accggcaagg agccgatgcg
    11881 ctggaaagtg ctccaggtcc tgtcggacga gatcgccctg gccagcgcgt ttcgtcagcc
    11941 ctgacaccct cccacctccc gagcgccccg ggcttcgggg accggtttac gggccgggat
    12001 ggtagcgcat gatcagcacc gtgatgtcgt cggactgttc acgcgcgccg ctgaaggcga
    12061 caacgtcatc cacgacgccc tgcaggacat ccgcgacgga tgccgtcgcc gggagcgagg
    12121 ccagccggcg gtccatgcgc gcctcaccgt agagctgatc agcctcgttg aacgcctcgg
    12181 tgatcccgtc ggtgtagagg atgagggtct cgcccggctg cagggtcagg atttcgcggg
    12241 caaaggtccg gccgtccatg gccccgacga cgatgttgct ccgggttcgc acaacggcga
    12301 caggcccgtc ggcggcccgg cgtcgcggcg ggttgtggcc gccgttggca aagaccacct
    12361 ccccggtgcg gacatcgagg atgccgacga agagcgtcac gaaggtgcag ctttcgttgc
    12421 ggatggcgag gtcgtcgttg acgcgccgca ggatttcggc gggatcgcgc cactgcttgg
    12481 cggcggagcg cagcaggacg accacggccg acatgaacat ggcggcgggc atgcccttgc
    12541 ccgagacatc gccgacggcg aagaacaggt ggtcggcgtc gagcggaaag tagtcgaaca
    12601 ggtcgccgcc ggcctgcttg gcggtcttca tgaccgcctg gagctgaacg cgcccgccta
    12661 actccgcggg gtcgaacggg ccgggcagga aggtcgcctg gatctggccc gcgatgcgca
    12721 gttcgctttc ggcgcgttcc cgggccgact gggtcagctt gagctgttcg atgtgctcgg
    12781 ccaggttcga ttgcatgcgg tcaaagtcgt aggcaaggat ggcggtttcc gccttggtcc
    12841 ggttgccgtc ggggaagagg gcgggatcca ggcggatgtc tttggagaac tcgccttccg
    12901 cgaggcggca ggtgaagttc gacaggcggt tcaggggccg ggtaatggaa tagctcgcgg
    12961 cgtagctgaa gatgacggcc aggatgaagc tggcggcgcc catcaggaag aagttcagaa
    13021 aggcgcggcg cttgagcgcc tccacttcgt aagcatggat gtcggcgcct acgatgaagc
    13081 ggcggccgga cgaatccttg aagcccatga cgaccgagcg cagcaggcca taatccgggt
    13141 cgggggtcgt ggagatgttg accttccagt ccttctggac ctcgtagatg ttgggggccg
    13201 gattcgtgta ctgcacccag tacggcgtgc cggtgtcggc ggaggcgacg aagtagtagc
    13261 gattgctctc ctggagcagg gcgtagaggt aggtgccgcc gatgttgcgc aggaagatgt
    13321 ccagccgccg cgtgttgttc cagtgctcat ccccggtgat gctgccggga tgctggacgc
    13381 gctcgaagta gtccgcgggc agaagctggg gcatcgtgga cgcggcgatg agcaggcgct
    13441 tgtcgatccc gtcctggatg gctttcacgt tctgcttgaa gccgtagatg gtgaagatgg
    13501 tggacgagaa cagggtgatg ccgaagaagg ggatgaagat tttgcgccgg atgggttgcc
    13561 ggcggtacca cgccaccagt ttcacatccg gcctcccccg ccgcgcgggc tgacggccca
    13621 gaaaagcagg gcgcaggagc cgaggacggc ggcgatggcg gagatgccga tgccgagact
    13681 gaagctggcg gccaggccga aggccatcgg tcccagggcc tcgccgccgg actcaaagag
    13741 gttgtagatg cccatggcct tgtccgcgcc gaagcggcgg acgcgcggta gcgaggagta
    13801 ataggaaagg ccctggttga agagcaggct ttcggtcagg ccgaagagga tgatggcggc
    13861 ataggcggcg gcgagggacc ggagcgcggc aaacagcagc agggcggcgg tcatgaccag
    13921 cgccccgatg ggcatggcgc ggcgcgcgcc gaaaagattc gtggccagcc gcgtgatggc
    13981 cgggccgaga tacacgctgg ccgcgccgaa cagcatgaag gccaggctga tctcgttgat
    14041 cgagcagcct tgggattcgg caaagagcgg gaacaggaac ccgagaaaca gcccgcaggc
    14101 cgtcacgggc aggaagatga agaggaagaa gctccatacg gcgcggtcga tgaaaaaggc
    14161 ccacggcgaa ggcgcgcccg tggcccggtc gccggccggc tcgcggcggc ggttccggat
    14221 cagggccagg gcggcgacgg ccgccagcac catcagcacg gcctggatgg cgaataccgg
    14281 ccgcatgccc acgcgcgcgg ccagcatgcc cccggagacc gagccgacgt tgatgccggc
    14341 gatgaccccg gcggtcaacg caatgatgcc ggattccttc cggccctcgt ccttttcaat
    14401 caggaagtag gtgcggaagg caatcatcag gaggcccatg cccatgccga cgagaacgcg
    14461 gccggcgatc aggcggacga acgtgttcga gagggcggta acgaccatgc cgccggtgat
    14521 gacgaggccg gccagcacga catccgtctt gatgcccgcg cggagccgga catggccata
    14581 gagcagggcg atcacggcgc cgcagagggt ttcgatcacc atgggcaggc cgataacgac
    14641 gtcgaacggg atgcggccga tggcgggggc cagcgacttg ctgtagagcg gcaggaagga
    14701 agcggagaga aacacgccga caatggccat gaacgacacg aaccgcacgg cgccttcgtc
    14761 gtagcgctgg ccggcggtgt cgtcgtggcg gtccagcacg tgggagctga agaagccgat
    14821 ttcgaagaag acaaacagca gcacgaagag gaccatggcc aggtccatca gcgtgctctt
    14881 gaagagggtc cggttggcct cgcgctgggc ggactggtcc gcgccgacct cgagcaggcc
    14941 gacggtgacc ccgttggaat tcacgagcgg caccacgccg ttgagccaca ccccgtagat
    15001 atcaacaacc tcgccgacgt agggcttgcc ggtccggacc acctcggcgt agatggattt
    15061 gctgtactgg tagttgtagg ggtagaagat gccgcgcagg ccatcgtggt aggccagggc
    15121 cgagagcttg tcgccgaaga ccttgtagac gccggaatag gtgccgggat cgatgacgcc
    15181 ttcgcgggtg atggtctggc aaagctcctt cagcacggcc tggtagtcgg cattcatgta
    15241 gtgcttcacg tgctggatgc ggtcgacggc atcggcatcc accaccaggg catgatcctg
    15301 gaatgtaatc agggcc
//
LOCUS       DAOOTK010000077        15233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2637_length_15233_cov_5.530834, whole genome shotgun sequence.
ACCESSION   DAOOTK010000077 DAOOTK010000000
VERSION     DAOOTK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15233
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2637_length_15233_cov_5.530834"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PK634_09320"
     CDS             complement(<1..493)
                     /locus_tag="PK634_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97975.1"
                     /translation="MRTRHGGRAAALTGTEWAGLFLALGIHGALAESLHTWRVPQDFG
                     SIQAAVDAATPGDTILVRGPEDEFSEPVVFEENVVVTKPLTLSWNREEDGGYLRPVIQ
                     ARHRWKPAITVAQAADVSLIGFCVRNPGGIGAWASRTRGLLIRDCVLENSRVGLGFDP
                     VKNR"
     gene            complement(638..1258)
                     /locus_tag="PK634_09325"
     CDS             complement(638..1258)
                     /locus_tag="PK634_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOW97976.1"
                     /translation="MIRHLLFDIGGVLLNLDYGPAVERVLPLCDPALGLNSRSFFGLV
                     ERDPMLMDYERGRVPPEEFYRHFAKKAGYRGGYGEFVEAWYHVLSENRPMIEFARELA
                     ARRPITLASNAGVIHHPRMFRDFPSLGFRRGEVISCDIGAVKPERAFYERTLDRLGRR
                     AEECLFLDDRPENVAGAEACGIRSILHTGAPSSIAAVRAALSEASR"
     gene            1257..1844
                     /locus_tag="PK634_09330"
     CDS             1257..1844
                     /locus_tag="PK634_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97977.1"
                     /translation="MAGILIHFPRTGEYSRRAPAGFVDSEPRAGVVWSPMRNSRSRRG
                     GCTAGLLLLASFLPALCPGPASALESARIVRMTHRVLEARRASASLMPDRLGPSDAGT
                     GQGGGEEFRVYWDPGPGGSLPGTVVTFEFRQAFHSLIRTLFIQYPFVVKGERCALFEV
                     GEPTIRRGGRVTAWRVRVSRGGRRLAERISESWEP"
     gene            1859..2404
                     /locus_tag="PK634_09335"
     CDS             1859..2404
                     /locus_tag="PK634_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW97978.1"
                     /translation="MPGSDGIQVAIQVPLAFIRVVGRGSFKISASLKEFAVHALDAGC
                     RRIVLDMAECVGMDSTFMGVLAGLAFRLRAVPEGRIVIVNLNPRTRGLLATLGLDEAV
                     ETHMAGATPADLEPLLARGEALTELPAEERGQAETARTMLEAHENLVRLSPENLPKFK
                     DVLTFLREDLKKSGGAAPGAR"
     gene            2476..2823
                     /locus_tag="PK634_09340"
     CDS             2476..2823
                     /locus_tag="PK634_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW97979.1"
                     /translation="MRECIIQTEQRDDLDILHLDGALDAYSFPRLETALSKLREQHRN
                     RVVLDCGGLDYISSAALGTLIGFARRAREGNGDLKLVALSKKIYTIVELLGFHKILEI
                     YANVDEAARRFAG"
     gene            2836..3306
                     /locus_tag="PK634_09345"
     CDS             2836..3306
                     /locus_tag="PK634_09345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW97980.1"
                     /translation="MTAENKKETGIRGYVTLHVPCRYSYLRVIRQAVLDLCARAGMTE
                     FKSAQLEMAVDESCSNIIEHGYAGESEADLEPRHPGLRLNLIQHTDRVIVEILDRGKA
                     FDFNERQVVEPDQYLEDERERGLGMYIIKRFVDEAEYEPATEAGNCLRLMKRIR"
     gene            3374..3934
                     /locus_tag="PK634_09350"
     CDS             3374..3934
                     /locus_tag="PK634_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOW97981.1"
                     /translation="MKKHSENKPCDAPAPAAAEAAPAPAGAGEEDVVSREIETLKDQM
                     LRLQADFDNYRKRALRDRQDQSARAAEDLLRALLPVSDHFEMGLKTAREHGADPAVVE
                     GFQLVLDQFQAVLRKAGLEPVPAEGQPFDPAVHEAVSHLPSETVPADAVIEQTRAGYR
                     LGGKLLRAAQVVVSRGPEAGPETEAE"
     gene            3934..5109
                     /gene="dnaJ"
                     /locus_tag="PK634_09355"
     CDS             3934..5109
                     /gene="dnaJ"
                     /locus_tag="PK634_09355"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOW97982.1"
                     /translation="MARGKRDYYEVLGVARGASADEIKKAYRKLAVQYHPDKNPGSVE
                     AEEQFKEISEAYEVLSDAEKRQRYDRFGHRAFGPGAGGFGFGGIDLEEALRTFMGAFG
                     GGGSIFENFFGGGGRGREEAGGPARGNDLRFDLEIDFEEAVLGSERELTLPILQECDA
                     CKGTGAAPGSKRETCRRCNGMGVTIASNGFFQIRQTCSACGGAGEIVSQPCRECRGEG
                     RVKGRSTISLKIPPGVETGSRLRLAGKGEGGLRGGPAGDLYVVLHVKPHELFQRRDED
                     IFVEWPIPYHVAALGGEVEVPTLHGYVPLKIPAGTQSGTLFRLKGKGVEGGHLLRGGD
                     QHVRVVIEPPVRLSGRQKDLLRELAGSFEEGNHPQAARLRKLAETFYERRKVLEQDG"
     gene            5106..5855
                     /locus_tag="PK634_09360"
     CDS             5106..5855
                     /locus_tag="PK634_09360"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOW97983.1"
                     /translation="MSEPGKTHARAFLPGAAWERGETELPADEGHHLARVLRVRAGDR
                     VEIFNGRGGEGAAEVLEVVKDRVRLRVLSRAQKPEPRPAVTLALAVLRETPMNWAVQK
                     AVELGAAAIAPVWTARGVVRPSGKENRCRRWEAIVLNAARQCRAAWLPSVEAPVELPA
                     FLARPREGLFIVAALEPGAVPLCRALAAAPAPERVTALVGPEGDFTPDELREARAAGA
                     VAVTLGARTLRAETAALYLLSALQHQWGTDG"
     gene            5891..6763
                     /locus_tag="PK634_09365"
     CDS             5891..6763
                     /locus_tag="PK634_09365"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOW97984.1"
                     /translation="MHAFGARFLLRAGWISLLVPLLVAAAEPAPRGFRVWSSDWPKEK
                     KLEAANVAVIGGMAVYGLLFWGYGGHTFQVVNERWFQRDSIHGGADKLGHAFSTYVAA
                     MSYGAVYENWGYDRREASLYGALSGLATFCMIEFGDAFSRHGFSFQDLTADAAGALWG
                     YLRRECPALRSLVDYRVSYWPSWGTWRGYTSDCISDYSGFKYLLAFKGSGIKALSSTW
                     LRYAEFHLGYYTRGYENQDWCHYGEPHRKAYVGIAWNLSDLLKQRNWRQAGTFLEYYQ
                     LPFTYLHQGFDLDR"
     gene            complement(6781..7128)
                     /locus_tag="PK634_09370"
     CDS             complement(6781..7128)
                     /locus_tag="PK634_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97985.1"
                     /translation="MNPSAPVRAIAVFMAGLSLSGCITFNHPKDHWLGLDKVGHFTTF
                     GALGAAAAAAALHHDESDNNAFMIGVGTAVGLGSVKEYVDEKPLHKYFSGKDLIADLA
                     GGIVGSLIVIQAD"
     gene            7198..7785
                     /gene="rsmD"
                     /locus_tag="PK634_09375"
     CDS             7198..7785
                     /gene="rsmD"
                     /locus_tag="PK634_09375"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009442445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOW97986.1"
                     /translation="MRITGGILKGRILKVPSGPLRPTQDKVRAALFSMLGEAVVGARV
                     LDLFAGTGALGLEAWSRGAAHVCWVDSDPAVLRVLRANVDSLCAPGGGRAECRRDEVF
                     RFLERPPAGGAFDLVLADPPYDREGTRRWLEKTLLALQTGPILAPEGLLAFEQSAGEP
                     VPAPAGWSLLRDRKYGETRLLLYRRQAGESGEKNT"
     gene            7782..8270
                     /gene="coaD"
                     /locus_tag="PK634_09380"
     CDS             7782..8270
                     /gene="coaD"
                     /locus_tag="PK634_09380"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOW97987.1"
                     /translation="MNRAAIYAGTFDPITLGHLDVIERAARIFNRLILAVAVSTRKAT
                     LFSVEERRALVRNVVRRFKNVEVDIFDGLLVQYARKKGVRVLVRGLRAFSDFEFEFQM
                     ALTNRKMAPDIETLFLMPKEDFSYVSSSTVREVAQYGGDTSSFVAPSVERALQAKLKP
                     RG"
     gene            8277..8744
                     /locus_tag="PK634_09385"
     CDS             8277..8744
                     /locus_tag="PK634_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOW97988.1"
                     /translation="MRIDIPKVPADGGWYEEDVPASVLELEADPRVRPGGPISCRFFV
                     QRVSGELVVKGRLGMPASLQCGRCGCFFSTTVSDLSFLRAYDAPEGTETVDLTPDIRE
                     DILLSLPAYPLCSSECRGLCAQCGHNLNEGPCPCKPPAVGPGKWSALDKLKEL"
     gene            8773..8958
                     /gene="rpmF"
                     /locus_tag="PK634_09390"
     CDS             8773..8958
                     /gene="rpmF"
                     /locus_tag="PK634_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005953147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOW97989.1"
                     /translation="MGVPKRKTSKSKRRMRQRSQKKAIVTARACPKCGAPQLSHRVCP
                     ECGTYKGRQVLIISADE"
     gene            8961..9962
                     /gene="plsX"
                     /locus_tag="PK634_09395"
     CDS             8961..9962
                     /gene="plsX"
                     /locus_tag="PK634_09395"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOW97990.1"
                     /translation="MRVAVDAMGGDFAPGAIVAGALEAARTLKGLSALILVGDEAAIR
                     RELDGKGPVPPVIQVRHASEVVGMEESPATAIRRKKDSSINRSVDMVKHGEADAVFSA
                     GNTGAAVASTTLKLRTLEGVVRPAIATVIPTPVKPFVMIDSGANTDSSPAMLAQFAVM
                     GSVYSREIIGRKEPKVGLLSIGGEDSKGNESTKEAFKILEKAPLHFHGNVESSDIYNG
                     VVDVVVCDGFVGNVVLKTSESVARTISRWIREECSKNPVRMLGALLARGAFGSIRKRS
                     DPATYGGAPLLGVGGTCLIGHGSSSPRAVCNGIRVAAESVSHHINQMIVDGIRNLGN"
     gene            9965..10987
                     /locus_tag="PK634_09400"
     CDS             9965..10987
                     /locus_tag="PK634_09400"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOW97991.1"
                     /translation="MKDTKRIHPAVVGRTVSIVGIGSYVPDRILTNADLEKMVDTTDE
                     WITTRTGIKERHIAAPDQASSDLGAEAARRALADAGISPEDVDMIVLGTITPDMGFPN
                     TACFVQAKIGARNAFCFDIEAACSGFLYALEIGRQFVSTGTMETVLVIAAEKITSILD
                     WKDRNTCVLFGDAAGAVVLRARGAAHGVMSSVLGSDGTLAELLMLPGGGSRYPATEET
                     VRQRLHYMKMAGREVFKHAVTNMSRAATDALRSSGLGTDDIRWIIPHQANLRIIEAIG
                     ERLGVGKDKVVVNLDRYGNSSAATIPLALDELYRQGKLQKHDKILMVAFGGGFTWGAS
                     VVEWDK"
     gene            11045..11977
                     /gene="fabD"
                     /locus_tag="PK634_09405"
     CDS             11045..11977
                     /gene="fabD"
                     /locus_tag="PK634_09405"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOW97992.1"
                     /translation="MSHTAILFPGQGAQAVGMGKDLAEAFPASRAVFDTANRVLGFDL
                     ARLCFEGPIEELTKSSNTQPAIFTVSAACFAALRAQRPDVAFAATAGLSLGEWSALWY
                     AGSISLEDALRVLQARGRFMQESCDERPGGMVSIIGAAPDKVQALCAATGVEVANFNS
                     ADQIVLSGPADRIAEAAKRAAEFGARKAVKLNVAGAFHSSLMASAARKLEALLAGVTF
                     KAPARPVFSNVSGRPHGSPDEIRQLMVQQVTSSVRWISCVEGMKAQGIRAHLECGPGK
                     VLAGLVKRIDKDAAVHNINDRPTLEGALNSGAIA"
     gene            11992..12735
                     /gene="fabG"
                     /locus_tag="PK634_09410"
     CDS             11992..12735
                     /gene="fabG"
                     /locus_tag="PK634_09410"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOW97993.1"
                     /translation="MGQLDGKIALVTGAARGIGQAIAVKLASEGADMALCDVQADWLA
                     ETAAKVAGLGRKAECIAVDVSKAEQVQAAIERAEKAFGRIDVLVNNAGITRDTFLVRM
                     SEEDWDQVLDINLKGAFLFIKAAARGMMKQRRGAIVNVASIIGLIGNAGQCNYSASKA
                     GLIALTKSVAREFASRGIRANAVAPGFIQTRMTDQLAEEIKNKMLGAIPLGRFGQPED
                     VANVVTFLASDASSYVTGQVLTICGGMVM"
     gene            12764..13006
                     /gene="acpP"
                     /locus_tag="PK634_09415"
     CDS             12764..13006
                     /gene="acpP"
                     /locus_tag="PK634_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOW97994.1"
                     /translation="MALEDKVKEIIVEQLGVNPEQVTPEASFIEDLGADSLGTVELVM
                     AFEEEFGAEIPDEDAEKLTSVGAVITYLKEKGFGAA"
     gene            13144..14385
                     /gene="fabF"
                     /locus_tag="PK634_09420"
     CDS             13144..14385
                     /gene="fabF"
                     /locus_tag="PK634_09420"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOW97995.1"
                     /translation="MTGRKVVVTGLGVVSPLGCDVQKFWERLQAGHCGIRRIQTFDAS
                     AYSAQIAGEVVEFDPAPYISRKEQRHMDPFSQYGVVAAKLAVADSGLVLDQVNRERAG
                     VVVGSGIGGLQVCYEQSRNLIERGPGRFSPFMIPQMIVNILSGQIAIEFGFQGPNFCV
                     VSACASATHCLGESLRMIQHDEADIMIAGGAEGSICELALGGFAAMRALSTRNDDPAT
                     ASRPFDKDRDGFVMAEGAGILVLEELEHAKKRGARIYCELAGYGRTCDAYHITAPAEG
                     GAHAARGMSLAMQDAGLTPADIDYINAHGTSTPLNDKGETAAIKAALGEARARQIMIS
                     STKSMHGHMLGAAGGVESIACAMTLKHGVIPPTINYTTPDPECDLDYVPNQARQAKVR
                     ACLNNSLGFGGHNATLCFKAV"
     gene            complement(14419..15177)
                     /locus_tag="PK634_09425"
     CDS             complement(14419..15177)
                     /locus_tag="PK634_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97996.1"
                     /translation="MRLGLLFFLLIASGSVASGDTIAFFYALDGDLAALKTEAREVGQ
                     PYAVGARRIQRLALGSHTIYALKMGSGCVETAASAQALLSRFRCDWAFSLGPAGALSE
                     NIETGRWYRADRVIAWQRESSTWETDWGRFPATSPSPLLQSTSAIAVASGEIFVSSSG
                     ERERIRAVTGADAVDMNSFGLALVCADHGVPLFNWKIISDRADENAPADFRAFVESYQ
                     GEGGRALADIISLLPANPRDPASYPAIEELLRNP"
BASE COUNT         2538 a         5047 c         5283 g         2365 t
ORIGIN      
        1 cgcggttctt cacgggatcg aagcccaggc cgacccggct gttttccagg acgcaatccc
       61 ggatcagcag gccgcgggtc cggctggccc acgccccgat gccgcccggg ttgcggacgc
      121 agaaaccgat gaggctcacg tccgccgcct gggccacggt gatcgcgggt ttccagcgat
      181 ggcgcgcctg gatcaccggc cgcaagtagc ccccgtcttc ctcgcggttc caggaaagcg
      241 tgaggggctt cgtcacgacg acgttctcct cgaagacgac cggctcgctg aactcgtcct
      301 cggggccgcg cacgaggatc gtgtccccgg gcgtggcggc gtccacggcg gcctggatgc
      361 tgccgaaatc ctgcggcacc ctccatgtat gaaggctctc ggccaacgcc ccgtggattc
      421 cgagcgccag gaacaagccc gcccattccg tgcccgtgag agccgccgcc cggccgccgt
      481 gtcgcgtgcg catggactcc tcccgtgatg acgatgccgt gatgaaagta atataaggga
      541 ggcggccgga gatgtatagc ttaatcgttt atgtttcgtg cgcgccggcc gggccgattt
      601 cacttttttt tgcgcgcgcg gcgtcccctg aaccccgtca gcgggaggcc tcggacagcg
      661 ccgcgcggac ggcggcgatg gaggaggggg cgccggtgtg caggatggat cggatgccgc
      721 acgcctccgc gcccgcgacg ttctccggcc ggtcgtcgag aaacaggcac tcctccgccc
      781 gccgccccag ccggtccagc gtgcgctcgt agaaggcgcg ctcgggcttg accgcgccga
      841 tgtcgcagga gatcacctcg ccccgccgga agcccagcga cgggaagtcg cggaacatcc
      901 gcgggtggtg gatcacgccc gcgttcgagg ccagggtgat cggccggcgc gcggccagtt
      961 cgcgcgcgaa ttcgatcatg ggcctgttct cggacagcac gtggtaccag gcctccacga
     1021 attccccata gccgccccgg tacccggcct tcttcgcgaa gtggcggtag aattcctccg
     1081 gcggcacccg gccgcgctcg tagtccatga gcatcgggtc gcgttcgacc aggccgaaga
     1141 aggaccggct gttcaggccg agcgcgggat cgcagagcgg caggacgcgc tccaccgccg
     1201 ggccgtagtc caggttcagc agcacgccgc cgatatcgaa gagcaggtgg cggatcatgg
     1261 ccggcatcct aatccatttt ccgcggacgg gcgagtattc ccgtcgcgcg ccggccggtt
     1321 tcgttgacag cgaaccccgc gcgggggtag tctggtcgcc catgcggaac tcgcgatcga
     1381 gaaggggcgg atgcacggcc ggtcttctcc tgctggcgtc gttcctgccc gcgctgtgcc
     1441 cgggcccggc gtcggcgctc gagtccgccc gcatcgtgcg gatgacgcat cgcgtcctgg
     1501 aggcccgccg cgcctcggcc tcgctgatgc ccgaccggct cgggccctcc gacgccggga
     1561 cggggcaggg gggcggcgag gagttccggg tgtactggga tcccgggccg ggcggcagcc
     1621 tgccgggcac ggtcgtgacg ttcgagttcc ggcaggcctt ccattccctg atacgcacgc
     1681 ttttcatcca gtaccccttt gtggtaaaag gagagcgatg cgccctgttc gaggtcgggg
     1741 agccgacgat ccggcggggc ggccgcgtga cggcgtggcg cgtgcgcgtc tcgcggggcg
     1801 ggcggcggct ggcggaacgg atttcggaat cgtgggaacc gtgacgagga actcgggcat
     1861 gcccggcagc gacggaatac aagtggcgat ccaggtcccg ctggcgttca tccgcgtcgt
     1921 gggccgcggc tcgttcaaga tcagcgcctc gctcaaggag ttcgccgtcc acgccctgga
     1981 cgccggctgc cggcgcatcg tgctggacat ggccgagtgc gtcggcatgg acagcacgtt
     2041 catgggcgtg ctcgcggggc tcgccttccg cctgcgcgcg gtgccggaag gccgcatcgt
     2101 gatcgtcaat ctcaacccgc gcacgcgcgg cctgctggcg acgctggggc tcgacgaggc
     2161 cgtggagacg cacatggccg gcgccacgcc cgccgacctg gagccgctcc tggcccgcgg
     2221 cgaggcgctc accgagcttc ccgcggagga gcgcggccag gccgagaccg cgcggaccat
     2281 gctggaggcg cacgagaacc tcgtccgcct ctcgccggaa aacctgccca agttcaagga
     2341 cgtgctgacc tttcttcggg aggacttgaa gaaaagcggg ggcgcggccc ccggcgcgcg
     2401 gtaggccccg acggcgacag ggtcttgatt gcgcgccgcc cgatgggtta ggatgggcgc
     2461 gtactggagg aacgtatgag ggaatgcatc attcagacgg agcagcggga tgacctggat
     2521 atcctgcacc tggacggcgc gctggatgcc tattccttcc cgaggctgga gaccgccttg
     2581 agcaagctcc gcgagcagca ccggaaccgc gtcgtcctcg actgcggcgg cctcgactac
     2641 atcagcagcg ccgcgctcgg caccctgatc ggattcgccc gccgcgcccg cgagggcaac
     2701 ggcgacctca agctcgtcgc cctctccaag aagatctaca ccatcgtcga actgctcggg
     2761 ttccacaaga tcctcgagat ctacgccaac gtggacgagg ccgctcgccg gttcgcgggc
     2821 tgaagggatg gagtcatgac cgcggaaaac aaaaaagaga cgggcatccg gggctacgtg
     2881 accctccacg tgccctgccg ctacagctac ctccgcgtga tccggcaggc cgtgctcgac
     2941 ctctgcgccc gcgccggcat gacggagttc aagtccgcgc agctcgaaat ggccgtggac
     3001 gaatcctgct ccaacatcat cgagcacggc tatgccggcg agtccgaggc ggacctcgaa
     3061 ccccgccacc ccggcctgcg cctgaacctg atccagcaca ccgaccgcgt gatcgtcgag
     3121 atcctcgacc gcggcaaggc cttcgatttc aacgagcgcc aggtcgtcga gcccgaccag
     3181 tacctcgagg acgagcggga gcggggcctg ggcatgtaca tcatcaagcg gttcgtggac
     3241 gaggcggagt acgagcccgc gaccgaggcc ggcaactgcc tgcggctgat gaagcgcatc
     3301 cggtaggctc cgccgcgacc ctcgcgggcg cgcgaacggc gccccggcgg aatccgggcg
     3361 cggagaatcc cttatgaaaa agcattccga aaacaagcct tgcgacgccc cggccccggc
     3421 ggccgcggaa gcggcgcccg ctccggccgg cgccggcgag gaggacgttg tctcgcgcga
     3481 gatcgagacg ctgaaggacc agatgctccg cctccaggcc gacttcgaca actatcgcaa
     3541 gcgcgccctg cgcgaccgac aggaccagtc cgcgcgcgcc gcggaagacc tgctccgggc
     3601 gctcctgccc gtctccgatc atttcgagat gggcctcaag accgcgcgcg agcacggcgc
     3661 cgacccggcc gtcgtggagg gctttcaact ggtgctcgac cagttccagg ccgtgctgcg
     3721 gaaggcgggc ctggagccgg tgcccgccga gggccagccg ttcgacccgg cggtccacga
     3781 ggcggtcagt cacctgccct cggaaaccgt gcccgccgac gcggtcatcg aacagacccg
     3841 cgcgggctat cgcctgggcg gcaagctgct gcgcgccgcg caggtggtcg tcagccgcgg
     3901 ccccgaggcc ggcccggaaa ccgaggcgga gtgatggccc gcgggaagcg agactactac
     3961 gaggttctgg gcgtcgcccg cggcgcctcg gccgacgaga tcaagaaggc ctaccgcaag
     4021 ctggccgtcc agtaccatcc cgacaagaac cccggcagcg tcgaggcgga ggagcagttc
     4081 aaggagatct ccgaggcgta cgaggtgctc tccgacgccg agaagcgcca gcggtacgac
     4141 cggttcgggc accgcgcgtt cgggccgggc gccggcggct tcgggttcgg cggcatcgac
     4201 ctcgaggaag ccctgcgcac gttcatgggc gcgttcggcg ggggcggcag catcttcgag
     4261 aacttcttcg gcgggggcgg tcgcggccgc gaggaggccg gcggcccggc gcgcggcaac
     4321 gacctccggt tcgacctgga gatcgatttc gaagaggccg tcctgggctc cgagcgcgaa
     4381 ctgaccctgc ctatcctcca ggagtgcgat gcctgcaagg ggaccggcgc ggcgcccggg
     4441 agcaagcggg aaacctgccg ccgatgcaac gggatgggcg tcaccattgc ctccaacggg
     4501 ttcttccaga tccgccagac ctgctccgcc tgcggcggcg cgggcgaaat cgtgtcccaa
     4561 ccgtgccggg aatgccgggg cgagggccgc gtcaagggcc ggtcgacgat ctcgctgaag
     4621 atcccgccgg gcgtcgagac cgggtcgcgc ctgcggctgg ccggcaaggg cgagggcggg
     4681 ctgcgcggcg ggccggcggg cgacctgtac gtcgtgctcc acgtcaagcc ccacgagctg
     4741 ttccagcggc gcgacgagga catcttcgtc gagtggccca tcccgtacca tgtcgcggcc
     4801 ctgggcggcg aggtcgaggt gcccacgctc cacggctacg tccccctgaa gatcccggcc
     4861 ggcacccaga gcggcaccct gttccgcctc aagggcaagg gcgtggaggg cggccacctg
     4921 ttgcgcggcg gagaccagca cgtccgcgtc gtcatcgagc cgccggtgcg gctctcgggc
     4981 cggcaaaagg acctcctccg cgaactggcc ggctccttcg aagagggcaa ccatccccag
     5041 gcggcccgcc tgcgcaagct ggcggagacg ttttacgaac gtagaaaagt cctcgaacag
     5101 gatggatgag cgagcccggt aaaacgcatg cgagggcgtt ccttcccggc gcggcgtggg
     5161 agcgcgggga aacggagctg cccgcagatg aaggccacca cctggcccga gtgctccggg
     5221 tgcgcgccgg ggaccgggtg gaaattttca acggccgggg aggggagggc gcggccgaag
     5281 tcctggaagt cgtgaaagac cgggtccgcc ttcgcgtcct gtcccgcgcg cagaaacccg
     5341 aaccgcggcc ggccgtcacg ctggccctgg cggtcctgcg cgaaaccccc atgaactggg
     5401 cggtccagaa ggccgtcgag ctcggcgcgg ccgcgatcgc gccggtctgg accgcgaggg
     5461 gcgtggtccg gccgtcgggg aaggagaatc gatgccgccg atgggaggcc atcgtcctca
     5521 acgccgcgcg gcagtgccgc gcggcctggc tgccgtccgt cgaggccccc gttgaactgc
     5581 ccgctttcct ggcccgtccg cgcgagggtt tgtttatcgt cgccgcgctg gagcccggcg
     5641 ccgttccgct gtgccgggcc ctggccgcgg cgcccgcgcc ggagcgcgtg acggcgctcg
     5701 tggggccgga gggagatttc acgcccgacg agttgcgcga ggcgcgagcg gccggcgccg
     5761 tggccgtcac gctgggggcg cgcacgctgc gcgccgagac ggcggccttg tatctgttga
     5821 gcgcgctgca gcaccagtgg gggacggacg gttgaacggt ggtccggcaa ggagcggtcg
     5881 ccggccgccg gtgcatgcat tcggggcgcg tttcctcctc cgggcaggct ggatcagcct
     5941 gctggttccc ttattggtcg cggcggccga gccggcgccc cggggcttcc gtgtatggag
     6001 ttcggactgg cccaaggaga aaaagctgga ggcggccaac gtggccgtca tcgggggcat
     6061 ggcggtctac gggctcctgt tctgggggta cggcgggcac acgttccagg tcgtcaacga
     6121 gcgatggttc cagcgcgatt cgatccacgg cggggcggac aagctcggcc acgccttcag
     6181 cacctacgtg gccgcgatgt cgtatggggc cgtgtacgag aactggggat acgaccggcg
     6241 cgaggcctcg ctgtacgggg ccctgtccgg gttggcgacc ttctgcatga tcgagttcgg
     6301 cgatgcgttc agccggcacg gtttctcgtt ccaggatctg acggcggatg cggccggcgc
     6361 cctgtggggc tacctccggc gcgagtgccc ggccttgagg agcctggtgg attaccgcgt
     6421 gtcctactgg ccgtcgtggg gcacgtggcg cggatacacc agcgactgca tcagcgacta
     6481 ctccggcttc aagtatctcc tggccttcaa gggcagcggg atcaaggccc tctcgtccac
     6541 gtggctgcgc tacgccgagt ttcatctggg atactacacc cggggctacg aaaaccagga
     6601 ttggtgccac tacggggaac cccatcgcaa ggcctacgtc gggatcgcct ggaatttgtc
     6661 cgatcttctg aagcagagaa actggcgcca ggcgggaacc ttcctcgaat actaccagct
     6721 gcccttcacc tacctgcacc agggcttcga tctcgaccgc tgaccggtta cgcgccggct
     6781 tcagtcggcc tggatcacga tcaggctgcc gacgattcct ccggcgaggt ccgcgatcaa
     6841 atccttcccg ctgaagtact tatggagcgg tttctcgtcc acgtactcct tgaccgagcc
     6901 cagtcccacc gccgtgccca cgccgatcat gaaggcgttg ttgtccgatt cgtcgtggtg
     6961 gagcgccgcc gcggcggccg ccgcgcccag cgcgccgaac gtcgtgaaat gtccgacctt
     7021 gtccaggccg agccaatggt ccttgggatg attgaacgtg atgcaacccg aaagagacag
     7081 gccggccatg aacaccgcga tggcgcgcac gggggcggac ggattcatgg acgatttctt
     7141 gaccaatgcg ccccaaccgt caatcattat tcctgaacct gaatcccgaa ccctcggatg
     7201 cgcatcaccg gcggcatatt gaaaggcaga atcctcaagg tcccgtccgg ccctctgcgg
     7261 ccgacgcagg acaaggtgcg cgccgcgctg ttttccatgc tgggcgaggc cgtggtcggc
     7321 gcgcgcgtcc tggacctctt tgccggcacg ggggcgctgg gcctggaagc gtggagccgc
     7381 ggcgcggccc acgtgtgctg ggtggattcg gacccggcgg tgctgcgggt cctgcgcgcc
     7441 aacgtggact ccctgtgcgc gccgggcgga ggccgggcgg aatgccgccg ggacgaggtg
     7501 ttccgttttc tcgaaaggcc gccggccgga ggcgcgttcg acctggtcct ggccgatccg
     7561 ccctacgacc gggaagggac gcggcgctgg ctggaaaaga ccttgctcgc cttgcaaacc
     7621 ggtcctattc tcgcccccga aggtttgctg gcctttgagc agagcgcggg cgagccggtc
     7681 cctgcgccgg cgggctggtc cctgctccgg gaccggaaat acggagaaac ccggctgctg
     7741 ttgtaccgtc gccaggccgg ggagtccggg gagaagaaca catgaaccga gcggccatct
     7801 acgcgggcac gtttgacccg atcacgctgg ggcacctgga cgtgatcgag cgcgccgcgc
     7861 gcatcttcaa ccggttgatc ctcgccgtcg cggtcagcac ccgcaaggcg acgctgttct
     7921 ccgtcgagga gcggcgcgcc ctggtgcgga atgtcgtccg gcggttcaag aacgtcgagg
     7981 tggatatctt cgacggcctg ctggtccagt acgcccggaa gaagggcgtg cgggtcctgg
     8041 tgcgggggtt gcgggcgttc tcggacttcg agttcgagtt ccagatggcg ctgaccaacc
     8101 gcaagatggc gccggacatc gagacgctgt tcctgatgcc gaaggaggat ttcagctacg
     8161 tcagttccag cacggtgcgc gaggtggcgc agtacggggg ggacaccagc agtttcgtcg
     8221 ccccttccgt ggagcgcgcg ctccaggcga agctgaagcc caggggctga cccgtcatgc
     8281 gcatcgacat ccccaaggtc cccgccgatg gcgggtggta cgaagaggac gtgccggcct
     8341 ccgtgctgga gttggaggcc gatccgcggg tccggcccgg gggcccgatt tcctgccggt
     8401 ttttcgtgca gcgggtctcc ggcgaactcg tggtgaaggg ccgcctcggc atgccggcct
     8461 cgctccagtg cggccggtgc ggctgttttt tctcgacaac cgtgtccgat ttgtctttcc
     8521 tacgcgcgta cgacgcaccg gaaggcacgg agaccgtgga tttgacaccg gatatccggg
     8581 aggatatcct gctcagtctt ccggcctacc ccttgtgttc ctcggaatgc aggggccttt
     8641 gcgcgcagtg cggccataac ctgaacgagg ggccgtgccc gtgcaagcct ccggcggtcg
     8701 ggccgggcaa gtggagtgcc ctggataaat tgaaggaact gtaacaaccg ggctgatcag
     8761 gagggttggg gaatgggcgt accgaaaaga aagacatcga agagcaagcg gcgcatgcgc
     8821 cagcggtcgc agaagaaagc cattgtgacc gcccgcgctt gtccgaaatg cggggcgccg
     8881 cagctgtccc accgggtctg ccccgagtgc ggcacgtaca aaggccggca ggtcctgatc
     8941 atttcggcgg acgaatagcc atgcgcgtgg ccgtggatgc catgggcggg gatttcgcgc
     9001 ccggggcgat cgtggccggc gcgctcgaag ccgcccgcac cctcaagggc ttgtcggcgc
     9061 tcatcctggt cggcgacgag gcggccatcc gccgcgagct cgacgggaag ggcccggtgc
     9121 cgcccgtcat ccaggtgcgc cacgcctccg aggtcgtcgg catggaggaa agcccggcca
     9181 cggcgatccg ccgcaagaag gattcctcga tcaaccgctc cgtggacatg gtcaagcacg
     9241 gcgaggccga cgccgtcttc tcggccggca acaccggcgc cgccgtcgcc tccaccaccc
     9301 tgaagctgcg cacgctcgag ggcgtggtgc ggccggcgat cgccaccgtg atcccgaccc
     9361 ctgtcaagcc gttcgtgatg atcgactcgg gcgccaacac ggattcgagc cccgccatgc
     9421 tggcccagtt cgccgtgatg gggtcggtct actcccggga aatcatcggg cggaaagagc
     9481 cgaaggtggg cctcttgagc atcggcggcg aggacagcaa gggcaacgag tccaccaagg
     9541 aagccttcaa gatcctcgag aaggcgccgt tgcacttcca cggcaacgtg gagagctccg
     9601 atatctacaa cggggtcgtg gacgtcgtgg tctgcgacgg gttcgtcggc aacgtcgtcc
     9661 tcaagaccag cgagagcgtg gcgcggacca tctcccgctg gatccgcgag gagtgctcga
     9721 agaaccccgt ccgcatgctc ggcgcgctgc tcgcgcgcgg ggcgttcggc tcgatccgca
     9781 agcgctcgga ccccgccacg tacggcggcg ccccgctgct cggcgtgggc ggaacctgcc
     9841 tgatcggcca cggctcctcc tcgcctcgcg ccgtgtgcaa cggcatccgc gtcgcggccg
     9901 agtccgtgtc gcatcatatc aaccagatga tcgtggatgg cattcgcaac ctggggaatt
     9961 agcattgaaa gacaccaagc gcattcatcc ggccgtggtc ggccgcacgg tctccatcgt
    10021 aggcatcgga tcctatgtcc ccgaccgcat cctgaccaat gccgacctgg aaaaaatggt
    10081 cgacacgacg gacgagtgga tcaccacgcg cacgggcatc aaggagcggc atatcgcggc
    10141 gcccgaccag gcctcctccg acctcggcgc cgaggccgcc cgccgcgcgc tggccgacgc
    10201 cgggatatcc ccggaggacg tggacatgat cgtgctcggg acgatcaccc cggacatggg
    10261 gttccccaac accgcctgct tcgtccaggc caagatcggc gccaggaacg ccttctgctt
    10321 cgacatcgaa gccgcctgct cgggcttcct ctacgcgctc gagatcggcc gccagttcgt
    10381 cagcaccggg acgatggaaa cggtcctggt catagcggcc gagaagatca cgtccatcct
    10441 cgactggaag gaccggaaca cctgcgtgct gttcggcgac gccgccggcg cggtggtcct
    10501 gcgggcgcgc ggggccgccc acggcgtgat gtcgtccgtc ctcgggtcgg acgggacgct
    10561 ggccgagtta ctgatgctcc ccggcggcgg aagccgctac ccggccacgg aggaaaccgt
    10621 gcgccagcgt ctccattaca tgaagatggc cggccgcgag gtcttcaagc acgccgtcac
    10681 caatatgagc cgcgccgcca cggacgcgct caggagcagc ggcctgggca ccgacgacat
    10741 ccgctggatc atcccgcacc aggccaacct ccgcatcatc gaggccatcg gcgagcggtt
    10801 gggcgtcggg aaggacaagg tcgtggtgaa cctggaccgc tacgggaaca gctcggccgc
    10861 caccatcccg ctggccctcg atgagttgta ccggcagggc aagctccaga agcacgacaa
    10921 gatcctgatg gtcgccttcg gcggcggctt cacctggggc gcgtccgtcg tcgagtggga
    10981 caagtagggc gtgcggaatg ccgaaggacg aaggcggaat ttcctgaacc ctaggagctg
    11041 ttccatgagc catacagcca ttctcttccc cggccagggc gcccaagcgg tcggcatggg
    11101 caaggatctg gccgaggcgt ttcccgccag ccgggccgtg ttcgatacgg ccaaccgggt
    11161 gctgggcttc gacctggcca gactctgctt cgaggggccg atcgaggaac tgacgaagtc
    11221 ctcgaacacg caacccgcga tcttcaccgt cagcgccgcc tgcttcgcgg cgctgcgggc
    11281 gcagcggccg gacgtcgcgt tcgccgcgac ggcgggcttg agcctgggcg agtggtcggc
    11341 gctctggtac gcgggctcga tctcgctgga ggacgccctg cgggtgctgc aggcccgcgg
    11401 gcggttcatg caggagtcct gcgacgagcg gccgggcggc atggtgagca tcatcggcgc
    11461 cgcgcccgac aaggtccagg ccctgtgcgc ggccacaggc gtcgaggtgg cgaacttcaa
    11521 ttcggccgat cagatcgtgt tgagcggccc ggcggaccgc atcgccgagg cggcgaagcg
    11581 cgccgccgag ttcggcgctc gaaaggccgt gaagctcaac gtggccggcg ccttccactc
    11641 gtccctgatg gcctccgcgg cgaggaaact ggaagccctg ctggccggcg tgacgttcaa
    11701 ggcgcccgcc cggccggtct tctccaacgt ctcgggccgg ccgcacggct cgcccgacga
    11761 gatccgccag ctgatggtgc agcaggtcac ctcgtcggtc cggtggattt cctgcgtgga
    11821 gggcatgaag gcgcagggga tccgcgcgca cctcgaatgc ggccccggga aagtgctggc
    11881 gggcctggtc aaacgaattg acaaggatgc cgccgtccat aacattaatg accgccccac
    11941 gctggagggg gccttgaatt cgggcgctat cgcctaggag cggatacgca tatgggacaa
    12001 ttggatggca agatcgcgct ggtgacgggc gcggcgcgcg ggatcgggca ggccatcgcc
    12061 gtcaagctgg cctccgaggg cgcggacatg gcgctttgcg acgtccaggc cgactggctc
    12121 gcggagaccg ccgccaaggt ggccggcctg ggccggaagg cggagtgcat cgccgtggac
    12181 gtctcgaagg ccgagcaggt gcaggcggcc atcgagaggg cggagaaggc gttcggccgc
    12241 atcgacgtgc tggtcaacaa cgccggcatc acccgcgaca cgttcctcgt gcgcatgagc
    12301 gaagaggact gggaccaggt cctggacatc aacctcaagg gcgccttcct gttcatcaag
    12361 gccgccgcgc gcggcatgat gaagcagcgc cgcggggcca tcgtgaacgt ggcctccatt
    12421 atcggcttga tcggcaacgc cggtcagtgc aactattcgg catccaaggc ggggttgatc
    12481 gctctgacca agtccgtggc ccgggaattc gcgtcgcggg gcatccgggc caatgccgtg
    12541 gccccgggct tcatccagac gcggatgacg gatcagctcg ccgaggagat caagaataag
    12601 atgttgggcg ccattccgct gggccgcttc gggcagccgg aggacgtggc caacgttgtg
    12661 acgttcctgg cgagtgacgc gtcgtcgtac gttacgggcc aggtgcttac gatctgtggt
    12721 ggcatggtga tgtgagagag aaacagaaag aggagaagaa cccatggcgt tggaagacaa
    12781 agtcaaagag atcatcgtgg agcagctcgg cgtcaatccc gaacaggtca cccccgaggc
    12841 ttcgttcatc gaggacctgg gcgcggattc gctcggcacc gtcgagttgg tcatggcctt
    12901 cgaggaagag ttcggcgcgg agatcccgga cgaggacgcc gagaagctga cctcggtcgg
    12961 cgcggtaatc acctacctga aggaaaaggg cttcggcgca gcctgattcc gtgtcgtagt
    13021 ttcaggtcgg gagctctccg cgcgggagtt cccggcctgc ctttcatttt ccatcggcat
    13081 cggcagggcg aaggcgcgga ataggatcgg cgcggttgag attcgtttga acagggggcg
    13141 gacatgacag gcaggaaagt agtggtaacg ggactgggcg tcgtttcccc gctggggtgc
    13201 gacgtgcaga aattctggga gcgccttcag gccggccact gcggcatccg ccgcatccag
    13261 acgtttgacg cgtcggccta ctcggcgcaa atcgccggcg aggtcgtcga gttcgatccc
    13321 gccccctaca tctccaggaa agaacagcgg cacatggacc cgttcagcca gtacggggtc
    13381 gtggccgcca agctggccgt cgcggattcc gggctggtcc tcgaccaggt caaccgcgag
    13441 cgcgccggcg tcgtcgtcgg cagcggcatc ggcggcctgc aggtctgcta cgagcagagc
    13501 aggaacctca tcgagcgcgg gccgggccgc ttttcgccgt tcatgatccc ccagatgatc
    13561 gtcaacatct taagcgggca gatcgccatc gagttcggct tccagggccc caacttctgc
    13621 gtggtctccg cctgcgcctc tgccacgcac tgcctgggcg agtcgctgcg gatgatccag
    13681 catgacgagg ccgacatcat gatcgccggc ggcgccgaag ggtcgatctg cgagctcgcg
    13741 ctgggcggat tcgccgccat gcgcgccttg agcacccgga acgacgaccc cgccacggcc
    13801 agccgcccgt tcgacaagga ccgcgacggc ttcgtcatgg ccgagggcgc cgggatcctg
    13861 gtcctggagg agctggagca cgcgaagaag cgcggcgcgc gcatctactg cgaactcgcc
    13921 ggctacggcc gcacctgcga cgcctaccac atcaccgccc ccgccgaagg cggcgcgcac
    13981 gcggcgcgcg ggatgagcct ggccatgcag gacgccgggc tgacgccggc cgacatcgat
    14041 tacatcaacg cgcacggcac cagcaccccg ctcaacgaca agggcgagac ggcggcgatc
    14101 aaggcggccc tcggcgaggc gcgggcgcgc cagatcatga tcagctccac caagtccatg
    14161 cacgggcaca tgctcggcgc ggcgggcggc gtggaatcca tcgcctgcgc gatgacgctg
    14221 aagcacggcg tgatcccgcc cacgatcaac tacacgacgc ccgacccgga atgcgacctc
    14281 gactacgtgc ccaaccaggc gcggcaggcg aaggtccggg cctgcctcaa caactccctc
    14341 gggttcggcg ggcataacgc gacgctctgc ttcaaggccg tgtaaaggat caaccccgtt
    14401 tcctcggcgc ggtgggggtc acgggtttcg aagtagttcc tcgatggcgg gatacgacgc
    14461 cggatcgcgg ggattggcgg gcagcaagga aataatgtcg gccagcgccc gtccgccctc
    14521 cccttggtaa ctttccacga aagcgcgaaa gtcagccggc gcgttttcat cggcccggtc
    14581 gctgatgatc ttccagttga acaggggcac gccgtgatcc gcgcagacca gggccaggcc
    14641 gaagctgttc atgtccacgg cgtcggcccc ggtgacggcg cggattcttt cgcgctcgcc
    14701 ggaggaagac acgaaaattt ctccggacgc cacggcgatg gcggaggtgg attgaagaag
    14761 cggggaaggg ctggtcgcgg ggaaacggcc ccaatccgtt tcccacgtcg aggactcccg
    14821 ttgccaggcg atgactcgat ccgcgcgata ccatcgcccg gtttcgatgt tttccgataa
    14881 agcgccggcc ggcccgagcg agaacgccca atcgcaccgg aaccgcgaga gcagcgcctg
    14941 cgccgatgcc gccgtctcca cgcaacccga gcccatcttc agcgcgtaga tcgtatgcga
    15001 gcccagcgcc aggcgttgga ttcgccgcgc gccgacggca tagggttgcc cgacttcgcg
    15061 ggcttctgtt ttcaacgccg cgagatcgcc atcgagggcg taaaaaaagg caatcgtgtc
    15121 gccgctcgcg acagatccgg acgcgatcag aagaaaaaaa agaagcccca gcctcatggt
    15181 ttcagcctaa ctcgatgaac cgtttaaggt aaaggaaaat gcggaaatac ctt
//
LOCUS       DAOOTK010000078        15133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2670_length_15133_cov_6.725759, whole genome shotgun sequence.
ACCESSION   DAOOTK010000078 DAOOTK010000000
VERSION     DAOOTK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2670_length_15133_cov_6.725759"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1136
                     /locus_tag="PK634_09430"
     CDS             249..1136
                     /locus_tag="PK634_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97997.1"
                     /translation="MTRQTIVACLAGALVLGGLSAAMAKDKEPAHKVTAQEVAEMLAD
                     PSATITYLNAAYRGYQNVGPENDMNQELRLNGAGFLKLPDNSSIFYRAYLPLYATEVV
                     PPYELPTRPGGSGAVVDRLENPFRFDNEGCGDTLLSAYWVPTAGTLILGYGAAMMVPT
                     ASEKYYGTEKWSAGPTLVAAKKVPGKYTVGGLLTHVWSFAGKDSRDGINMTTIQPAGT
                     LFLNKKGTSLTVGSETTFNWDAEDDAWQVPVTVGLGQILPPIGKQFIGVAVAGSYYIE
                     KPGMVQDWDVRAVVSVVFP"
     gene            1216..1947
                     /locus_tag="PK634_09435"
     CDS             1216..1947
                     /locus_tag="PK634_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97998.1"
                     /translation="MKTARWASWFVVAGVLVLAVAARAGGEEWSFEITPYAWLVNVDG
                     RVSFGDREADFSADFQDLFEYVDFAGSLLGTASRGRWVGFAQADFFTLHRDFKREPGG
                     TFESDLLFLNTMAGYRFDGFKSGSTVEVLAGLRYMRLDGRIEITGAGAADRSPDALDG
                     SVMLHGSFPLRFISEKLRLNTALSVGAGDSDLVWSLQPDLQYHFNERFSARAGYRRLQ
                     YEFSKEGADVDMGFQGFLAGVSVAL"
     gene            1981..3705
                     /locus_tag="PK634_09440"
     CDS             1981..3705
                     /locus_tag="PK634_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW97999.1"
                     /translation="MKHHYSRRLFVVWGLLSALYAAGPAHGQWISETFYLTNGWNAVY
                     LRGTPWPDTLDAQFAGLPIRAVHRNYLQYDTTQFAESAADLPTRGTEWLIWYPSNSPH
                     RVLTTLWKLNGNTPYLIECTTNCAWTPTGVPVIPARLWLPNTWNFVGLPVNPGSGVTF
                     AEFFENAHNIDVSPTPAGGKVFRTKTDGTQQDITSQTAILAINPKEACWILTEGLSAF
                     IGKIFAHATLGSLRYPPGVSINSFSVWNTCGSNQQVSVELVASEAPPAGAPERMGDVP
                     LLHFAYDYDDGRYEWTPLAQGAPLTKELSSNEEWVVTLAVDRSSLSAPPSTNSTWQSV
                     LAVTDGGGTLIRVPVVAEYGAESPYDALWPYGLWVGEVTLDKVTQLADGAETDPVMAA
                     GELEMRLIVHIGTNGQARLLQQVVLELAHSVSGGVTNNFYRLRASDRGLAATGEASRV
                     SSVAFPYGLNVAFDGDPQVELTTSYILDFNSAVNPFKHLYNPNHDNVSAAGLALAEGG
                     ESYTISNDVSLVFDPVPPYSPSSSLWNPEEELTGQYVQTMHGLRNEAVEVEGRFTLRR
                     VSRTGVME"
     gene            3746..4801
                     /locus_tag="PK634_09445"
     CDS             3746..4801
                     /locus_tag="PK634_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98000.1"
                     /translation="MIHRKQRIRAAGLVVALGAWAGAAGAIGVPAFMNYQGVLANPQG
                     TAFTNQTVSVNFRIYDAVRGGTMIWGTRQVVTTDSNGIFNVELGDAGENVPGATNRVA
                     SILEVFTGTNAANRWLEIEVEYNKSSAMSPRQPFLTAPYAYQAGSAAGSAGDFTVGDV
                     LTVESNAVFQRAVILQDTGENALAFHGAVSDHGSLTVSQKVSAASHLQVKGGLTVHGN
                     AAFSNTVDFTENVEFDGPVVFSNGVSFRGHTAMFGAGRVLAAHAAGSSSSTNGVVPTN
                     GFVSVNMLVTDNDNGTLTFSLGSTQWLFRSHWDTGLTDDMDLKTLTLFPVKGGTTWSL
                     NAAGDKIGFNLYYWPIP"
     gene            4818..5792
                     /locus_tag="PK634_09450"
     CDS             4818..5792
                     /locus_tag="PK634_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98001.1"
                     /translation="MNIRRKGGGGILAAWLACGAALAQVPNQLYMQGVFLQADGATPA
                     PGAHSVSVTIRSNAAVALTRTTNVTANNHGLADFVIADAALPALFRGTTNASFVMTGG
                     ATQAFVTAPYAFQAGNLPSASGNFTVQGNLNVASNATLTALTAENGGTLAVPITVARY
                     ATFTNLNGVEFGAALDVAGGVTVAGPAEANYDTRFTNTAATTVFLGATNEVVLSNATV
                     RSSFTLLSTNYTTTGTSGTAPADGFLMVWVKTDNHKTSGVNVKIDTLTFKLQWYCDVE
                     LSSQNLNLYKGSAFPVAKDVTWSVMLVNASDSDKITITCYWVPLNGEG"
     gene            5798..6874
                     /locus_tag="PK634_09455"
     CDS             5798..6874
                     /locus_tag="PK634_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98002.1"
                     /translation="MKTIIMTGVVLLLGLAAGAQHVPDRLNYQAVLTDEHGNPRASVT
                     NGVRFRIYDEAEGGNLIWGEKHSVTTTPLGLFNVVLGNGIALGETMEAATLREAFASG
                     TLVEQRYLELQSLNDDGSAQSPILPRQRFLTVPYVFQANDAQEAAGDFQVSGALYSTR
                     VGMEVGRLVATNPAGSITVAGDLRVDGYGHSDVTATFSSNSSVKSTAPDALIARSNLT
                     VNAAWTVAGTATFYRGVSARDPILKKGARVRGGVRALGSYQSLGTTLTNLSSRTAAGD
                     GFALVYFKTDGWGDNADLVVTVKTNQYRLQHNPSSGAAHTYDLHYLDTACIPIPSGSV
                     WSVNFQDGNASHSKFDAYWIPFGY"
     gene            6887..>15133
                     /locus_tag="PK634_09460"
     CDS             6887..>15133
                     /locus_tag="PK634_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOW98003.1"
                     /translation="MKTRIPRFTLRSRIGLWGCGLALGLACAASARAQTVSGNDAIRF
                     PAALPFVNGRPDLSSVPVQAGGHVGGVWATNNLGATNLVLKSSIFGHNVEHLKPDAYL
                     GQALNPPAAISNVAVAWSAMTNASLITPSGNAFFEPSVRTIYAAAGGEVVIRWKYEDG
                     TEVDTTYIVEAAPAGRPYRVYWTDRGAPKVNLNGKFVRFHYNDQVTAPQWVVTTNEND
                     VVVSNVLAGLYIDDSGGVNAVGGQQGMIVMQYFKTGMYNDQINTGSVIVIEVMPPDNQ
                     TLDARLGERILPQGGWAAVGELTPEITAGLMGENPYLYQHTSSDGSPVPKQDWLFPIR
                     RSVDDPWNIEIYWKVADLVGTRWFYEVDDYAAEWPECRKFVRGDGPDYGAALYFPSNL
                     TPSIMAYQEPEDGCATMSDPTTLITTTNEGRCLVQLLGDDDNVWFLPLELITRDYPAF
                     DLNPTEWMISSEIQPFATTRSILFPEGEGAQAVLAPSGMPLATNFTLECWVMPGNLED
                     QTNTEMAIFDKNTPGGPAWSNQVRLVIQSGASASAGTLTASLGSGRTNSPWLATMTST
                     NRVQSFRWSHLAFTLRGRTLSLYVDGSLGCRTTLGTNGVLQTNSSPIYLGNLTGTNSA
                     PFDGFIDNIRVWDIGLSSTQVQTAIEGNHHGYTMATLTNQGLKAYFPINALDSLAHLH
                     DLTGRYNGTATNRCYLPDVGAIGMRDSAAYSETHGYIYEPFGTSYNTSLYHYASVSDP
                     TAQTAIYGVNTNSDIEVWWSEKIQYPGMPTPFYIPNWVQRYQNVWPATNESPHIVLAS
                     GQGSKLGGLYEQGACVVFTNDGPAGSVILRHDTYFEGDFTVGAWIYLRSLPAAGQIAP
                     IVSFGNGPSDSVDRVHLAISSTGTLYLGTANGATHGLASAAVVPSPTGRWVYVLASLD
                     GSTATLYADNQLLATWPGLPVPDGVLRTNCYIGSDTLEERFHGKIDEVRVWSAVLDED
                     QRHDAMYHVVSGLDAEDLTLYFPFDAYKGNLIVEQVSGRKALVYNAVLEEPGAPAEGS
                     RIYSVDEQAALYYQNDASLPGFNPNEEHAILYREDGGDVVYAFRDDLNAAFNESEPFV
                     LVNYIDPETGRPAMDALRVVRTSEVYTAFAEATFAGQALEGPAPLDRVPGHWTGRDIR
                     ESGNAWKARDNMWFALAGGVHPTDTAAVIMHNYYPMQPGFWFPGIPTNRMPAEGVAIP
                     WLPELTAPDYQRSSYPTSGMPIAVDWTISWPEAIPVLDAGDTLIEARDDLPDIWNQLS
                     VDVVYQQSSANGGGRSVFLFDPICARGVTLTNSLESLGFETGGEEPSIYMRNGYYYFH
                     GLPPDLSERFYYNPNLSQSNLVLIGEYVTPLTGSGYLLVNELDDAEREILTDLVDTGN
                     AHHADWVRAIGLLAPDAVEVEANEPFAGLALHAPGDGAGYVTLAFNNASNPAMGVSQG
                     TPISLSLIRVSTNLATGRVIPIEDGYNLLSEQMSLLMSLDFAGEPGNYMFEWRWTEPN
                     ADGTTPTDPLACEIYTNGAGLNSLILNGQAPEDLINRFFAVRYRAQSNTVTHVVGTNW
                     SEFTEFSLAEGWVQRILNALTPFEQRMRDLYNNPVETQVSMISQAGPPYEGDVALNQE
                     NLENVGLIQLYQTVLNRAERVLDMAGLHDAAANQQLLLAASRLNDLYMLLGNEAYADA
                     LDPTIGFGSTMTLNNAAVLPVDYGSWASSLFCFDNQTASLIEEELALLRGRANPSLSP
                     GMGVSPVYNRLFWNFTKGITAGEVAYAVNYDITGHTNAIIDAVTAKQRFPQGHGDAWG
                     YYLDALTVYYKLLRHPTFDWGVPSITPMLMNDVTIDADYFDEKKFAEAAAALARAGVD
                     ILQRTYRQSYDESQDGLFPGYADSNTNRAWGVGQWGTRIGVGALCNWAVGNSLLPASA
                     DWQTNVASLVLAGAGHAQLPTNAYFNGDFTVEFWIKTADTGGSAEMDLARFSNLAGDN
                     VEIKLMSGVEPALLVAVTNTWSTAYSGATIETGVWTHVAGVLDGHVGRIYVNGLLVKE
                     QGGMESPTAIQRPSCTMGLGDGSELENFHGRFCELRVWSEARTAGEITGAMRQRLTGA
                     EAGLVAYWPVNEGEGTLLLDHAPGGRHAHAVDTTSWTNDVVTLAAMNEYDDPGILRID
                     RSTVSALAEITANLRSAQAAVDNADRGLNPLGLARTSIPFDIDPLDIDAGGSHFEQIL
                     ARAKTALNNAKTVFDGVQEATRLLRQQQQSANNFQAAAISQEADYTRRIIEIYGYPYS
                     DDIGTGKTYPQGYDGPDLYHYMYVDMSALGWSTEEVEPIAARTYEFIGDGADSILNVY
                     GFATNEPPGEEEEGVTNMITFHYANNGLPCKPTEWTGNRRAEGRLQIAYAEFLKQWLA
                     YLQALEVYEAKTEYLVEVFDWYTQTFAPYKDTDYDNSVAVKTLEEAHNTLAAIAKIKK
                     LVHEWKEEVHKGNVQVSVSAQIANQIMGVASGGDIFSLLRSVIVGTGKAIEAAMSVKT
                     YILEMIVSADKWLWKTTEYGAELAKLTHEWKLETIEQETELGELVRAQSQQLAATKVA
                     FQGMVQACQNALAIEQEGQRLLASRQRDRLEVANRVASMRYSDMAFRLFRNDALSRYS
                     SAFDLAAMYTYLAAKAYDYETGLLPAETATDPGSEFLGDVVRARTLGRIVTGEPQVGG
                     PVGEPGLADILARMSANWTVLDGRLGFNNPSWRRTWFSLRTELLRILPGTSGDAAWRT
                     ALNSYKVDDLFAVPEFRRYCIPFAAEGGLRAKEPALV"
BASE COUNT         2672 a         5122 c         5055 g         2284 t
ORIGIN      
        1 cgaggacacc ggcacccccg tgaacatgga ctacgacgtt ccgttcaagt tcacgggcac
       61 gatcgagaag ctggtggtca aactggtcct cccccagatg tcgccggccg accagatgaa
      121 gatgcagcag gcggcgatga aggcgaagct cgccgaatga tggggcggcg gcgcaacgat
      181 gtgaaaggcc cggaagcggc gcgagccgag cgggccggat agcgaaaaac aaagaaaggg
      241 aacatagcat gacaaggcaa accatagtgg catgcctggc cggggcgctg gtcctgggcg
      301 ggctgtccgc ggcgatggcc aaggacaagg agcccgcgca caaggtgacg gcgcaggaag
      361 tggcggagat gctggcggat ccctcggcca cgatcacgta cctgaacgcg gcctaccgcg
      421 ggtaccagaa tgtcgggccc gaaaatgaca tgaaccagga gctccggctg aacggcgccg
      481 ggttcctgaa gctcccggat aattcctcga tcttctaccg cgcctacctc ccgctgtacg
      541 cgacggaggt cgtgcccccg tacgaactgc ccacccggcc gggcggcagc ggcgcggtcg
      601 tggatcggct ggagaacccg ttccggttcg acaacgaggg gtgcggcgac accctgctct
      661 ccgcgtactg ggtgcccacg gccggcacgc tgatcctcgg ttacggcgcg gcgatgatgg
      721 tgccgacggc gtccgagaaa tactacggca cggaaaaatg gtcggcgggg cccacgctcg
      781 tcgcggccaa gaaggtgccg ggcaagtaca cggtcggcgg cctgctgacg cacgtctggt
      841 ccttcgcggg caaggacagc cgcgacggca tcaacatgac gaccatccag ccggccggga
      901 cgctcttcct caacaagaag ggcacctcgc tgaccgtcgg gtcggagacc acgttcaact
      961 gggacgcgga ggacgacgcg tggcaggtgc cggtgacggt ggggctcggg cagatcctcc
     1021 cgccgatcgg caagcagttc atcggcgtcg ccgtggcggg cagctactac atcgagaagc
     1081 ccgggatggt tcaggactgg gacgtccgcg ccgtggtctc ggtcgtcttc ccgtaaggtc
     1141 ggtcgagcgg aaaactgaag agcggcgagg ccggcgaccc cggtctcgcc gttcggtatg
     1201 aaagaaagga atgtcatgaa gaccgcgcgg tgggcaagct ggttcgttgt cgccggggtg
     1261 ctggtcctgg ccgtcgccgc ccgcgcgggc ggcgaggagt ggtcgtttga aatcaccccc
     1321 tacgcgtggc tcgtcaacgt tgacggccgc gtgagtttcg gggaccgcga ggcggatttc
     1381 agcgccgatt tccaggacct ctttgaatac gtggatttcg cgggctcgtt gctgggcacc
     1441 gcgtcgcgcg gccgctgggt cggctttgcc caggcggatt tcttcacgct ccatcgcgac
     1501 ttcaaacggg aaccgggcgg cacgttcgag tcggacctgc tgttcctgaa taccatggcg
     1561 gggtaccggt tcgacggatt caagagtggt tcgacggtgg aagtcctggc cgggctccgt
     1621 tacatgcggc tggacgggcg catcgagatc acgggcgcgg gggccgccga ccggtcgccc
     1681 gacgcgctgg acggcagcgt catgctgcac ggcagtttcc ccctgcggtt catctcggaa
     1741 aagctgcgcc tcaacacggc gctgtcggtc ggcgcgggcg actcggatct ggtctggagc
     1801 ctgcagcccg acctgcaata tcacttcaac gaacggtttt cagcgcgggc gggctaccgg
     1861 cggctgcagt acgagttcag caaggaaggg gcggacgtcg atatgggatt ccaggggttc
     1921 ctggccggag tgagcgtggc tttgtaagcc cggcgtcgta gttacaaggg aggcgagtcc
     1981 atgaagcatc attactcgcg gcgactcttc gtcgtgtggg gcctgctatc ggccctttac
     2041 gcggccgggc cggcgcacgg ccagtggatt tccgagacct tttatcttac gaacggctgg
     2101 aacgccgtct acctgcgcgg cacgccgtgg cccgacacgc tggacgcgca gttcgcgggc
     2161 ctgcccatcc gcgccgtcca ccgcaactat ctccagtacg acacgaccca gttcgcggag
     2221 agcgccgctg acctgccgac gcgcggcacg gagtggctca tctggtatcc ctccaactcg
     2281 ccgcaccgcg tgctgacgac cctgtggaag ctgaacggga acacgcccta cctgatcgaa
     2341 tgcacgacga actgcgcgtg gacgcccacg ggcgtcccgg tcatcccggc gcgcctgtgg
     2401 ctgccgaaca cctggaactt cgtcgggctg cccgtgaacc cgggcagcgg ggtgacgttc
     2461 gcggaattct ttgagaatgc gcacaacatc gacgtgtcgc cgaccccggc cggcgggaag
     2521 gtgttccgca cgaagacgga cggcacgcag caggacatca cgtcgcagac ggccatcctg
     2581 gcgatcaacc cgaaggaggc ctgctggatc ctgacggagg ggctctcggc gttcatcggc
     2641 aagatcttcg cgcacgccac gctgggctcg ctgcgctacc cgccgggcgt cagcatcaat
     2701 tccttttcgg tctggaacac ctgtggcagc aaccagcagg tgtcggtgga actggtggcc
     2761 agcgaggcgc cgccggccgg cgcgccggag cggatggggg acgtgccgct gctgcacttc
     2821 gcgtacgact acgacgacgg ccggtacgag tggacgccgc tggcgcaggg cgcgccgctg
     2881 acgaaggagc tgtccagcaa cgaggagtgg gtggtcacgc tggccgtgga ccgctcgtcc
     2941 ctttccgcgc cgccgtccac caactcgacc tggcagagcg tgctggcggt gacggacggg
     3001 ggcggcacgc tgatccgcgt gcccgtggtc gcggagtacg gcgcggaaag cccgtacgac
     3061 gcgctgtggc cgtacgggtt gtgggtcggc gaggtcacgc tggacaaggt cacgcagctg
     3121 gccgacggcg ccgagaccga cccggtcatg gcggccggcg agctggagat gcgcctgatc
     3181 gtgcacattg gcacgaacgg gcaggcgcgg ctcctgcagc aggtcgtcct cgagttggcg
     3241 cacagcgtga gcggcggcgt gacgaacaac ttctaccgcc tccgcgccag cgaccgcggg
     3301 ctggccgcga ccggcgaggc gtcgcgcgtc agcagcgtgg cgttcccgta cgggctcaac
     3361 gtcgcgttcg acggcgatcc gcaggtcgag ctgacgacgt cgtacatcct cgactttaac
     3421 agcgcggtca acccgttcaa gcatctgtac aacccaaacc acgacaacgt gagcgcggcg
     3481 gggctggccc tggccgaggg cggggaaagc tacacgattt ccaacgacgt cagcctggtc
     3541 ttcgacccgg tcccgccgta ctcgccgtcg tcgagcctgt ggaatcccga ggaggaactg
     3601 accgggcagt atgtccagac gatgcatggc ctgcggaacg aggccgtcga ggtggaaggc
     3661 cggttcacgc tgcggcgcgt gagccggacg ggggtaatgg aatgacgcgc gccggccggc
     3721 accggccggg gagggaagcg aacacatgat ccatcgcaaa caacgaatcc gggcggccgg
     3781 gctggtcgtc gcgctcggcg cctgggccgg cgccgcgggc gccatcggcg tcccggcgtt
     3841 catgaactac cagggcgtcc tggccaatcc gcaggggacc gcgttcacca accagaccgt
     3901 ctcggtcaac ttccggatct acgacgccgt gcgcggcggg acgatgatct gggggacgcg
     3961 ccaggtggtg accacggatt cgaacgggat cttcaacgtc gagctgggcg acgcgggcga
     4021 gaacgtgccc ggcgcgacca accgggtggc gtccatcctc gaggtgttca ccggcacgaa
     4081 cgcggccaac cgctggctcg agatcgaggt ggaatacaac aagtcgtccg ccatgtcgcc
     4141 gcgccagccg ttcttgaccg cgccctacgc ctaccaggcc ggcagcgccg ccggcagcgc
     4201 gggcgatttc acggtgggcg acgtgctcac ggtggagtcg aacgccgtct tccagcgggc
     4261 cgtgatcctc caggacacgg gcgaaaacgc cctggcgttc cacggggccg tgtcggacca
     4321 cggcagcctg acggtgagcc agaaggtgtc ggcggcctcg cacctgcagg tcaagggcgg
     4381 gctgaccgtc cacggcaacg ccgccttttc caacaccgtg gacttcaccg agaacgtgga
     4441 gttcgacggc ccggtggtgt tcagcaacgg ggtgagtttc cgcggccaca cggcgatgtt
     4501 cggcgcgggc cgcgtcctgg ccgcccacgc ggccggctcc tcctcctcga ccaacggggt
     4561 cgttccgacc aacggtttcg tgtcggtgaa catgctcgtc accgacaacg acaacggcac
     4621 gctcaccttc agcctgggga gcacccagtg gctgttccgg tcgcactggg acaccgggct
     4681 cacggacgac atggacctga agacgctcac gctgttcccg gtcaagggcg gcacgacctg
     4741 gtccctcaac gcggccggcg acaagatcgg tttcaacctg tactactggc ccattcccta
     4801 gcgagcgagg tgcgaccatg aacataagac gaaaaggcgg cggcgggatc ctggcggcgt
     4861 ggctggcctg cggcgcggcg ctcgcccagg tgccgaacca gctctacatg cagggcgtct
     4921 tcctccaggc ggacggggcc acccccgcgc cgggcgcgca ctccgtgtcc gtgacgatcc
     4981 gcagcaacgc ggccgtggcc ctgacgcgga cgaccaacgt gacggccaac aaccatggcc
     5041 tggcggactt cgtcatcgcg gacgcggcgc tgccggcgct cttccgcggc accaccaatg
     5101 cgtcgtttgt gatgaccggc ggggccacgc aggccttcgt caccgccccg tacgccttcc
     5161 aggccggcaa cctgccctcg gcgtcgggca acttcaccgt ccagggcaac ctgaacgtcg
     5221 ccagcaacgc cacgctcacc gcgctgaccg cggaaaacgg cggcacgctg gcggtcccga
     5281 tcaccgtcgc ccgctatgcg acgttcacca acctcaacgg cgtggagttc ggggcggccc
     5341 tcgacgtggc cgggggcgtg accgtcgccg gcccggccga ggcgaactac gacacgcgct
     5401 tcacgaacac ggcggccacg acggtcttcc tgggcgccac caacgaggtg gtgttgagca
     5461 acgcgacggt ccgctcctcc ttcacgctgc tctcgaccaa ctacacgacc acggggacga
     5521 gcggcacggc gccggccgac gggttcctga tggtctgggt caaaaccgat aaccacaaga
     5581 catccggggt caacgtgaag atcgacacgc tgaccttcaa gctgcagtgg tactgcgacg
     5641 tggaactctc gagccagaac ttgaacctct acaagggctc cgccttcccg gtggccaagg
     5701 atgtgacgtg gtcggtcatg ctggtcaacg cctccgactc cgacaagatc accatcacgt
     5761 gttactgggt tccgctgaac ggggagggat gaatcccatg aaaaccatca tcatgaccgg
     5821 cgtcgtcctc ctgctcggcc tggcggccgg ggcgcagcac gtgcccgacc gcctgaacta
     5881 ccaggcggtc ctcaccgacg agcacggcaa cccgagggcg agcgtcacca acggcgtccg
     5941 cttccgcatc tacgacgagg ccgagggcgg caacctgatc tggggcgaga agcattcggt
     6001 gaccaccacg cccctcgggc tcttcaatgt cgtgctcggc aacggcatcg cgctcggcga
     6061 gaccatggag gcagccaccc tccgcgaggc ctttgcctcc ggcaccctcg tggagcagcg
     6121 ctacctggaa ctgcagtcgc tgaacgacga cggctccgcc cagtcgccga tcctgccgcg
     6181 gcagcggttc ctgacggtgc cctacgtctt ccaggccaac gacgcccagg aggcggcggg
     6241 cgatttccag gtgagcggcg cgctgtacag cacccgcgtg ggcatggagg tcgggcggct
     6301 cgtcgcgacg aatcccgcgg gctccattac ggtcgccggg gacctccggg tggacgggta
     6361 cggccactcc gacgtgacgg cgacgttcag cagcaactcg tcggtgaaat ccaccgcgcc
     6421 cgacgccctg atcgcgagga gcaacctgac ggtcaacgcg gcctggaccg tggccgggac
     6481 cgccaccttc taccgcggcg tctccgcgcg cgacccgata ctgaagaagg gcgcccgcgt
     6541 ccggggcggc gtccgcgctc tcgggagcta ccagtccctc ggcacgaccc tgacgaacct
     6601 ctcctcgcgc acggccgcgg gcgacgggtt tgccctggtc tacttcaaga cggacggctg
     6661 gggggacaac gccgacctcg tggtcaccgt gaagaccaac cagtaccggc tgcagcacaa
     6721 tccctcgtcg ggtgccgccc acacgtacga cctgcattac ctggataccg cctgcatccc
     6781 cattcccagc gggtccgtct ggtccgtgaa cttccaggat ggaaatgcct cgcactccaa
     6841 gttcgacgcg tactggatac ccttcggcta ctgatcggag ccccgcatga aaacacgaat
     6901 cccgcggttt acgctacgat cccggatcgg cctgtgggga tgcggcctcg ccctcggcct
     6961 ggcctgcgcc gcgtccgcgc gcgcgcagac cgtgtccggg aacgacgcca tccgtttccc
     7021 cgccgccctg ccgtttgtca acggccggcc ggacctttcc tccgtgcccg tccaggccgg
     7081 gggccacgtg ggcggcgtgt gggccacgaa caacctcggg gcgaccaacc tcgtgctcaa
     7141 gtcttccatc ttcggccaca acgtcgagca cctgaagccc gacgcttacc tcggccaggc
     7201 gctgaatccg ccggcggcca tctccaacgt cgcggtggcc tggtcggcca tgacgaatgc
     7261 ctcgctgatc acgccctccg ggaacgcctt cttcgagccc tcggtccgga ccatctacgc
     7321 ggccgcgggc ggggaagtgg tgatccgctg gaagtacgag gacgggacgg aggtggacac
     7381 cacctacatc gtcgaagccg cgcccgccgg acggccgtac cgcgtgtact ggacggaccg
     7441 cggcgcgccg aaggtcaacc tgaacggcaa gtttgtccgg ttccactaca acgaccaggt
     7501 caccgccccg caatgggtcg tcacgaccaa cgaaaacgac gtggtcgtga gcaacgtgct
     7561 cgccgggctg tacatcgacg attccggcgg cgtgaacgcg gtcggcgggc agcagggcat
     7621 gatcgtcatg cagtacttca agaccggcat gtacaacgac cagatcaata ccggcagcgt
     7681 gatcgtgatc gaggtcatgc cgccggacaa ccagacgctg gacgcccggc tcggcgagcg
     7741 cattctcccg cagggcggct gggccgccgt aggcgagctg acgccggaaa tcacggccgg
     7801 gctgatgggc gagaatccat acctctacca gcacacatcg tccgacggct cgcccgttcc
     7861 caagcaggac tggctcttcc cgatccggcg gtccgtggac gatccgtgga acatcgagat
     7921 ctactggaag gtcgccgacc tggtggggac gcggtggttc tacgaggtgg atgactacgc
     7981 cgcggaatgg ccggagtgcc gcaagttcgt gcggggcgac ggccccgact acggcgcggc
     8041 gctctacttc ccgtcgaacc tgacgccgtc gatcatggcg taccaggagc ccgaggacgg
     8101 ctgcgcgacg atgtcggatc cgaccacgct gatcacgacc accaacgagg gccgctgcct
     8161 ggtgcagctg ctgggcgatg atgacaacgt ctggttcctt ccgctggagc tgatcacgcg
     8221 ggattacccg gccttcgacc tgaatccgac cgagtggatg atcagcagcg agatccagcc
     8281 cttcgccacg acccgctcga tcctgttccc ggaaggggag ggcgcgcagg cggtcctggc
     8341 gccgtcgggc atgccgctgg ccacgaactt cacgctggaa tgctgggtca tgcccgggaa
     8401 cctcgaggac cagaccaaca ccgagatggc catcttcgac aagaacacgc ccggtggccc
     8461 ggcgtggagc aaccaggtcc gcctggtcat ccagtccggc gcctccgcct cggccggcac
     8521 gctgacggcg agcctgggct ccggccggac caactcgccg tggctcgcga cgatgacgtc
     8581 gaccaaccgc gtgcagtcct tccggtggtc ccacctggcg tttacgctgc ggggccggac
     8641 gctgtccctg tacgtggatg gctccctggg ttgccgcacg accctgggca cgaacggggt
     8701 gctccagacc aacagttcgc cgatctacct cggcaatctc accggcacga acagcgcgcc
     8761 gttcgacggc ttcatcgaca acatccgcgt gtgggacatc gggctcagct cgacgcaggt
     8821 gcagaccgcc atcgagggca atcaccacgg atacaccatg gccaccctga cgaaccaggg
     8881 cctgaaggcc tacttcccga tcaatgcgct ggactcgctg gcccacctgc acgacctgac
     8941 ggggcgctac aacggcacgg cgaccaaccg gtgctacctt cccgacgtcg gcgcgattgg
     9001 catgcgcgat tccgcggcct actcggagac gcacggctac atctacgagc cgttcgggac
     9061 gtcctacaac acctcgctgt accactatgc cagcgtctcc gacccgacgg cccagaccgc
     9121 gatctacggc gtgaacacga acagcgacat cgaggtctgg tggagcgaga agatccagta
     9181 cccgggcatg ccgacgccct tttacatccc caactgggtg cagcgctatc aaaacgtctg
     9241 gcccgcgacc aacgagagcc cgcacatcgt gctggccagc gggcagggca gcaaactggg
     9301 cggcttgtat gagcagggcg cctgcgtggt gttcaccaac gacggccccg cgggcagcgt
     9361 gatcctgcgt cacgacacgt acttcgaggg cgatttcacc gtgggcgcgt ggatctacct
     9421 gcggtccctg ccggccgccg ggcagatcgc cccgatcgtc tcctttggca acggcccgtc
     9481 ggacagcgtg gaccgtgtgc acctcgcgat ctcctcgacc ggcaccctct acctgggcac
     9541 ggcgaacggc gccacgcacg ggcttgccag cgcggccgtc gtcccgtccc cgaccggccg
     9601 ctgggtgtac gtcctggcct cgctggacgg gtccacggcc acgctctacg ccgacaacca
     9661 gctgctggcg acctggcccg gcctgccggt cccggacggc gtcctgcgga cgaactgcta
     9721 catcgggtct gacaccctcg aggaacgttt ccacgggaag atcgacgagg tccgcgtctg
     9781 gtccgccgtg ctcgacgagg accaacggca cgacgcgatg taccacgtcg tgtcggggct
     9841 ggacgccgag gacctgacgc tgtacttccc gttcgacgcc tacaagggca acctgatcgt
     9901 ggagcaggtt tccggccgca aggccctggt ttataacgcg gtgctcgagg agccgggcgc
     9961 cccggcggag ggctcgcgga tctacagcgt ggacgagcag gcggccctct actaccagaa
    10021 cgacgcctcc ttgccgggct tcaatccgaa cgaggagcat gcgatcctgt accgcgagga
    10081 tggcggcgac gtcgtgtatg cgttccgcga tgatttgaat gccgcgttca acgagtccga
    10141 gccgttcgtc ctggtgaact acatcgatcc cgagacgggg cggccggcga tggacgccct
    10201 acgcgtcgtg cggaccagcg aggtgtacac ggcgtttgcg gaggccacct tcgcggggca
    10261 ggcgctggag gggccggccc cgctggaccg ggtgccgggc cactggacgg gccgcgatat
    10321 ccgcgaatcc gggaatgctt ggaaggcgcg ggacaacatg tggtttgcac tggccggcgg
    10381 agtgcatccc accgacaccg cggccgtgat catgcataat tattatccca tgcagcccgg
    10441 cttctggttc cccgggatcc cgaccaaccg gatgcccgcg gaaggcgtgg cgatcccctg
    10501 gctgcccgaa ctcaccgccc cggattacca gcggagcagc tacccgacca gcggcatgcc
    10561 gatcgccgtg gactggacga tttcctggcc ggaggccatc cccgtgctcg acgccggcga
    10621 caccctgatc gaggcccggg acgacctgcc ggatatctgg aaccagttga gcgtggacgt
    10681 ggtctaccag cagtcctccg ccaacggcgg cggccgcagc gtgttcctct tcgaccccat
    10741 ctgcgcgcgc ggggtgacgc tgaccaactc gctcgaatcg ttagggttcg agacgggcgg
    10801 ggaggagccg agcatctaca tgcgcaacgg ctactactac ttccacggcc tgccgcccga
    10861 cttgagcgag cggttttact acaatcccaa cttgagccag agcaacctcg tgctgatcgg
    10921 cgagtacgtc acgccgctga ccgggagcgg ctacctgctg gtgaacgaac tcgacgacgc
    10981 cgagcgcgaa atcctgaccg acctcgtgga caccggcaac gcgcaccacg ccgactgggt
    11041 ccgggccatt ggcctgctgg ccccggacgc ggtggaagtc gaggccaacg agccgttcgc
    11101 cggcctcgcc ctgcacgcgc cgggcgacgg ggccgggtac gtgaccctgg ccttcaacaa
    11161 cgccagcaac ccggcgatgg gcgtcagcca gggcacgccc atcagcctgt cgctgatccg
    11221 ggtctcgacc aacctcgcca cgggccgggt catcccgatc gaggacgggt acaacctgct
    11281 ctccgagcag atgagcctgc tcatgagcct cgacttcgcg ggcgaaccgg ggaactacat
    11341 gttcgagtgg cgctggacgg agccgaacgc cgacggcacc acgcccaccg acccgctggc
    11401 ctgcgagatc tatacgaacg gggcgggcct caacagcctc atcctcaacg gccaggcgcc
    11461 ggaggacctg atcaaccgct tcttcgccgt ccgctaccgc gcgcagtcga acacggtcac
    11521 gcacgtcgtc ggcacgaact ggagcgagtt cacggaattc agcctggccg agggctgggt
    11581 gcagcggatc ctgaacgcac tgacgccgtt tgagcagcgc atgcgcgacc tgtacaacaa
    11641 cccggtcgag acgcaggtca gcatgatcag ccaggccgga ccgccgtacg agggcgacgt
    11701 ggcgctgaac caggagaacc tcgagaacgt cgggctgatc cagctctacc agaccgtgct
    11761 caaccgcgcg gagcgggtgc tggacatggc cgggctgcac gacgcggccg ccaaccagca
    11821 gctcctcctg gccgcgtcgc gcctgaacga cctctacatg ctgctcggca acgaggccta
    11881 cgccgacgcg ctggacccga ccatcggctt cggctcgacg atgacgctca acaacgccgc
    11941 cgtcctgccg gtggactacg ggtcgtgggc gtcctcgctc ttctgcttcg acaaccagac
    12001 cgcctcgctg atcgaggagg aactggccct gctgcgcggg cgcgccaacc cctcgctctc
    12061 gccgggcatg ggcgtgtcgc cggtgtacaa ccgcctgttc tggaacttca ccaagggcat
    12121 cacggccggc gaggtcgcgt acgccgtgaa ctacgacatc accggccaca ccaacgcaat
    12181 cattgacgcc gtgacggcga agcagcgctt cccgcagggc catggcgacg cgtggggcta
    12241 ctacctcgac gcgctgacgg tctactacaa gctcctgcgg cacccgacgt tcgactgggg
    12301 cgtgccgtcc atcacgccga tgctgatgaa cgacgtgacc atcgacgcgg actacttcga
    12361 cgagaagaaa ttcgccgagg ccgccgccgc gctggcacgt gcgggcgtgg acatcctcca
    12421 gcgtacgtac cggcagagct acgacgagtc gcaggacggc ctgttcccgg gctacgcgga
    12481 cagcaacacg aaccgcgcct ggggcgtcgg gcagtggggg acccgcatcg gcgtcggcgc
    12541 gctctgcaac tgggcggtcg gcaactccct gctgccggcg tcggcggatt ggcagaccaa
    12601 cgtggcctcg ctcgtcctgg ccggggcggg ccacgcgcag ctgccgacca acgcgtattt
    12661 caacggcgac ttcactgtgg agttctggat caagacggcg gacaccggcg gctcggccga
    12721 gatggacctg gcccggttct cgaacctggc gggcgacaac gtggaaatca agctcatgtc
    12781 cggcgtggag ccggccctcc tcgtggcggt cacgaacacg tggtccacgg cgtacagcgg
    12841 cgccacgatc gagacgggcg tgtggacgca tgtcgcgggc gtgctggacg ggcacgtcgg
    12901 ccgcatctac gtcaacgggt tgctcgtgaa ggaacagggc ggaatggagt cgccgacggc
    12961 catccagcgc ccgagctgca ccatggggct gggagacggc agcgaactcg agaacttcca
    13021 cggccggttc tgcgagctcc gggtctggag cgaagcccgc accgccgggg agattacggg
    13081 cgccatgcgc cagcgcctga cgggcgccga ggccggcctc gtggcgtact ggcccgtcaa
    13141 cgagggcgag gggacgctcc tgctggacca tgcccccggc ggccgccacg cccacgccgt
    13201 ggatacgacg tcctggacca acgacgtcgt caccctggcc gctatgaacg agtacgacga
    13261 tcccgggatc ctgcggatcg accgctccac ggtcagtgcc ctggccgaga tcacggccaa
    13321 cctgcgctcg gcgcaggccg cggtggacaa tgccgaccgg ggcctgaacc cgctcggcct
    13381 ggcgcgcacg tccatcccat tcgacatcga tccgctcgac atcgacgcgg gcggctcgca
    13441 cttcgagcag atcctcgccc gcgccaagac cgcgttgaac aacgcaaaga ccgtcttcga
    13501 cggcgtccag gaggccaccc gcctgctccg gcagcagcag cagagcgcca acaacttcca
    13561 ggcggccgcc atcagccagg aggcggatta cacgcgtcgg atcatcgaaa tctacggcta
    13621 cccgtactcc gacgacatcg gcacggggaa gacctatccc caggggtacg acggcccgga
    13681 cctctaccac tacatgtacg tggacatgag cgcgctcggc tggagcacgg aggaggtcga
    13741 gcccatcgcc gcgcggacgt acgagttcat cggcgacggc gcggactcca ttctcaacgt
    13801 gtacggcttc gcaacgaacg agccgcccgg cgaggaggaa gagggcgtga cgaacatgat
    13861 cacgttccac tacgccaata acgggttgcc gtgcaagccg accgagtgga cggggaaccg
    13921 ccgcgccgag ggccggctgc agatcgccta cgcggaattc ctgaagcagt ggctcgcgta
    13981 tctccaggcg ctggaggtgt acgaggcgaa gaccgagtac ctggtcgagg tgttcgactg
    14041 gtacacgcaa accttcgcgc cgtacaagga caccgactac gacaacagcg tggccgtgaa
    14101 gaccctggag gaggcccaca acacgctcgc ggccatcgcg aagatcaaga agctcgtcca
    14161 cgagtggaag gaagaggttc acaagggcaa tgtccaggtc tcggtgagcg cgcaaatcgc
    14221 gaatcagatc atgggcgtgg ccagcggggg agacatcttc tccctgctgc ggtccgtcat
    14281 cgtgggcacg ggcaaggcga tcgaggcggc catgtcggtt aagacgtaca ttctcgagat
    14341 gatcgtcagc gccgataaat ggctctggaa gacgaccgag tacggggccg agctggccaa
    14401 gctcacgcac gagtggaagc tggaaaccat cgagcaggaa accgagctgg gcgagctggt
    14461 ccgggcgcag tcgcagcagc tcgcggccac caaggttgct ttccagggca tggtccaggc
    14521 ctgccagaac gcgctggcca tcgagcagga agggcagcgc ctgctggcga gccgccagcg
    14581 ggaccggctg gaagtcgcca accgcgtggc ctcgatgcgc tacagcgaca tggccttccg
    14641 cctcttccgc aacgacgcct tgagccggta cagttcggcc ttcgacctgg cggcgatgta
    14701 cacgtacctg gccgcgaagg cctacgacta cgagacgggc cttctgcccg cggagacggc
    14761 caccgatccc ggcagcgagt tcctgggcga cgtcgtgcgc gcgcggacgc tcggccgcat
    14821 cgtgaccggg gagccgcagg tcggcgggcc ggtcggcgag ccgggcctgg cggacatcct
    14881 ggcgcgcatg agcgccaact ggacggtgct ggacgggcgg ctgggcttca acaacccgag
    14941 ctggcgccgg acgtggttct ccctgcgcac ggaattgctc cggatcctgc ccgggacctc
    15001 gggcgacgcg gcgtggcgca cggcgctcaa ctcgtacaag gtggacgacc tctttgccgt
    15061 gccggaattc cgccggtact gcatcccgtt cgcggccgag ggcgggctgc gggcgaagga
    15121 gcctgccctg gtc
//
LOCUS       DAOOTK010000079        15100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2676_length_15100_cov_6.007245, whole genome shotgun sequence.
ACCESSION   DAOOTK010000079 DAOOTK010000000
VERSION     DAOOTK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_15100_cov_6.007245"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PK634_09465"
     CDS             complement(<1..610)
                     /locus_tag="PK634_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98004.1"
                     /translation="MTPKDLSFAKDHGKPDPVLEGPRRLLSRPLGLAPMADDLFAAAR
                     GSSRKIWSLMAGAMEIRREPRVDPAASRVRPDWPDEVPADIRPALDAFLLSAAEARFR
                     LDRAGASLEPGDADYIAASFFAGTFNAEDRAETRRDMEAAGFSGDLVRRVIAESDTLD
                     PSPAASNFLARVQRVSRPDLFKAGRGFHQAAEALAGSVERSAK"
     gene            complement(988..1965)
                     /locus_tag="PK634_09470"
     CDS             complement(988..1965)
                     /locus_tag="PK634_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW98005.1"
                     /translation="MRGPGEKDKLDPARPGRYRACRLCPRRCGVDRVAGVAGRCGETA
                     ACRVASYGPHFGEEPSFSGTRGSGAIFFSGCSSGCFFCQNYQISIEHRGVEMTVDELL
                     AAALSMAGPRGVHNLNFVTPDHFWPHIRELCARVRAAGADVPFLFNSSGYQDPGLVEE
                     YAGVMDMFMPDFKFAEPELARRCMGDEDYPRLALESLRRMVGAKGFLAPWDPTGSEPA
                     RQGVLVRHLILPGHVENSLAVLDLLREEFGTSLPISVMSQFRPVPACGARGELDRGLT
                     AEEHRRVTGRVGELGFKHVYVQHLRDTADFMPDFRRRAVFSGNRRRGPG"
     gene            2026..3138
                     /locus_tag="PK634_09475"
     CDS             2026..3138
                     /locus_tag="PK634_09475"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOW98006.1"
                     /translation="MFLPHASVTEILSAAATLNVSPGDTLMVLVAEHNAPDLDELRNA
                     LRQTIPRFFGGIFPAVLSGARAHDEGAVILALPALEPPRVCAVSARAGPSGADLALPT
                     ADPCTAVLLADGMSPGISEFLAGVFRQLGPATHCVGGGAGVSAMVQRPCVFTAEGVFD
                     NAAVMAFAPWRCSLGVQHGMKRLVGPIVATRTRQNIIEELNWRPALEVYKAIVERDTG
                     QPLTAENFYDSGRAWAYPFGLVREGAEDVVRDPLAVVDHGIRCAGGVPENTALHVLKG
                     DADHLIAAARRAGERAAAGARSRPRAALICDCVSRRMFLKGRFAEELSAVSAALRPID
                     HLEPEGMLTIGELSSSGESVLDFYNKTIVVGLLCDA"
     gene            3131..5218
                     /locus_tag="PK634_09480"
     CDS             3131..5218
                     /locus_tag="PK634_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW98007.1"
                     /translation="MPDPAANDVVGTISLLYELALSMGQSLDLDANCDLFIRTLLTRK
                     NLAFGAVWLKASALKNGATAEADSGSYRLVYANPRVRAREDRLPANHPLPARLGNMPF
                     FSVAANEPGFAEAVTENGIQGGAYTVYALGDIGFLKLYSVLRRSAVSPVEVNSLRQVV
                     AKFAVSIEGCLAHRQLARQIEERDRAEGERRTLAMVVEQMEDCVIITDAAGVFRYVNP
                     AFERVTGYAAREVAGLTPRLLKSGRHLPEFYAALWKTVSSGRPWRDRIVNRLKNGSLA
                     TFDTIVAPARDEKGRPAGYVAVLRDVTRLMEVEQQYAHAQKMESIGRLAGGIAHDFNN
                     TLTAVLGFGSLLLETTPEDGPDRHAVEQIVLAGERAANLTRQLLTFSRPPMPEMRVMD
                     LNALALNATHLLRRSLGEDVEVVTLLDEHTGSVRADVSLLNQIITNLALNARDAMPRG
                     GRLCLRTAPVELDAAFCAEHAGLAPGPHILLSVRDTGTGMTEEVRRRAFEPFFTTKRT
                     GKGTGLGLSVVYGIVRRLGGCVELDSRPDAGTEVRIWLPRVEARGDTLPVELEEKVVG
                     GRETILVVEDEPMVRDLATRLLRSLGYSVLEVGGGEEALSLLRRYHDRLDLVLSDVVM
                     PRMDGVELTLQLRQERPELQVLYMSGFADDAFAERGLNLDPRRLIRKPFTKETLARKL
                     REILDDRGSGANA"
     gene            complement(5237..7195)
                     /locus_tag="PK634_09485"
     CDS             complement(5237..7195)
                     /locus_tag="PK634_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98008.1"
                     /translation="MSRAAKVRLAGALLLLGLLLAAYGPSLRGGFLWDDDVYVAGNEL
                     LTAPDGLRRIWFSTDAPSQYFPLTYTLMRAERSALGLDPLGYHLVSVGLHGANALLAA
                     LVLGLLAAPGAWLAAALFALHPVQVESVAWITEQKNLLSTLFYLAALWAWIRSLEVDG
                     RRRRWFGAGVFAFYLLALFAKTTACTLPAALVLAAWRKGIRLTPARRLQVAALLGIGL
                     AMGLFTAWWEQHLQGSRDAGVRFPFLDCVLIASRALWFYAGKLLWPVRLEFSYSRWAI
                     DASDPRQYGWLLAGLAAAVGLALLWRRGIRGPALAALFFVACLSPLLGFIPLYTFQYS
                     FVADHYQYLACLGPIALATAGAAGLARRVGLPAWSRRALAALPILLLAALTRQQAHAY
                     RDIETLWRDTIAKNPASWMARDNLGCELAAQDRFGEAVESFRAALQIHPRSARTHFNL
                     ANTLGKMGRGPEALAAYDRALAEDPLHAKSRFNKAHLLASLGRAEEAAAGYEEALRVA
                     PGMAVAHVNLALLLAGQGRVGEAEEHYREALRLEPDNPDACNAYGMLLAARGDLAAAQ
                     ELYGQAIRLRPDHAHAHNNLANALAREGRWAEAVPHYEAALQADPGLAEAHFNFAAAL
                     RELGRPDEARVHIEEAVRLRPDLAGRAP"
     gene            complement(7192..9375)
                     /gene="rpiB"
                     /locus_tag="PK634_09490"
     CDS             complement(7192..9375)
                     /gene="rpiB"
                     /locus_tag="PK634_09490"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOW98009.1"
                     /translation="MSGDSTEHVLFVCTGNICRSPMAERLLRARLGPAAGVTVGSAGV
                     AAPNGAPASPEAVEVMARRGLDLKAFRSRMLTAELVRAATLIVVMTEAHRQAVARAFP
                     GASGKVRLLTSFGAGGGERDIPDPIGSPVGVYQRTSEQIDSAISDLVLYLVEQRGVRP
                     AAPKGAGMKIAIGSDHAGFELKEALKKLLAERNIRAEDVGAPSAESVDYPDYAAAVAR
                     RVSEGSVDQGILVCATGVGMSITANKFPGARAALCFTPAMARLARVHNNANILVLGGS
                     LLKPADAASMLDAWLSAEFEVGSRHERRVRKMAAIEALQSDPVAVNAVDPELYAALRK
                     EARRQRENLELIASENYVSPAVRAAQGSIMTNKYAEGYPGKRWYNGCEFVDEAERLAI
                     DRAKQLFGAEHANVQPHCGSGANMAVYFAMLQPGDTMLAMSLACGGHLTHGHKVNFSG
                     RFFNVVHYGVDPQTERIDYDEIAALAKEHKPKMICAGASAYSRIIDFKRLREIADAAG
                     AFLMVDMAHIAGLVAAGCHPNPVPFSDFITTTTHKTLRGPRGGMILCRERFAQDIDRQ
                     IFPGTQGGPLMHVIAAKAVCFHEALQPAFKTYQQQIVRNAQVLAAALEQRGLRIVSGG
                     TDNHMMLVDLTPMGVTGKDAATALDKACITVNKNAIPFDKQSPFVTSGIRVGTPAVTT
                     RGMKEPEMGKIAEFIGRVLSAPEDAGALQAVRADVVALTARFPVP"
     gene            complement(9413..10747)
                     /locus_tag="PK634_09495"
     CDS             complement(9413..10747)
                     /locus_tag="PK634_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOW98010.1"
                     /translation="MKSILAFVLGAGFAAGRAWAQEPAAPDPATSPAPAAVAAETAPA
                     PAAPPEAPPSLKRDAVPAVEQLAALLESYGLTLDADSARRAAVSAMVKSADPGGLWLN
                     EAEKSALEDRLQGFVFETGLRIRSADGMPKVAEVVAGSPAEAAGLQPGDRLEFIDGGA
                     AASLKDWEIRSLLRGGPDSRFRVQFLRGAATQSVELAAARIPQPSVAVAEDLPTGIGY
                     LRLNGLYDGASKEVIPALRGWDGTNYCGIIVDLRGAGGTNLAAAADAARLFAPEGALL
                     FSLRMAKGQDIEAFRAPGGSGLDAPVMVLIDGETEGACEALAAVFADSLRGAMLIGSA
                     TRGDPAVREVVSCPGGGYLYLATRQLVMADGLVLNGRTAVEPDVTVEGVPGDEYEPEP
                     DSRNGRLLAEEKEHRVLRARVRGDPALGRAADILLALKALNVRGKPPPEKEP"
     gene            complement(10744..11262)
                     /gene="purE"
                     /locus_tag="PK634_09500"
     CDS             complement(10744..11262)
                     /gene="purE"
                     /locus_tag="PK634_09500"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOW98011.1"
                     /translation="MRKAKTEVAVLMGSDSDWPVVEHAAKTLREFGVGFEARVLSAHR
                     TPDAAAAYARSAARRGLKVLVAAAGGAAHLAGAVAANTILPVIGIPVSGGALDGLDAL
                     LATVQMPSGVPVATVALGKAGAVNAALLAVQILALGRPDLRRRLEAHKRTMKKKVAAG
                     NRRLKAAAEKTT"
     gene            complement(11259..12530)
                     /gene="purD"
                     /locus_tag="PK634_09505"
     CDS             complement(11259..12530)
                     /gene="purD"
                     /locus_tag="PK634_09505"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOW98012.1"
                     /translation="MRILIVGGGGREHALAWKISRDSCRPELFCAPGNAGTARLAENL
                     MIPASDLDSLVAWARAARPDLTVIGPEAPLCAGLADRLQAVGLRVFGPGRSAARLEGS
                     KAFAKEIMEKAGVPTARAARFTDANQALSYIRSQPGRVVVKADGLAAGKGVTVCDTPD
                     EAEKAVEDALVNRAFGEAGREVLIEERLEGEEVSVLALVDGSHIEMLASAQDHKRVGD
                     GDTGPNTGGMGAYSPAPVLTPAQWQAVRRDVFERTIGELSRRGITYRGVLYAGLMLTT
                     GGPKVLEFNCRFGDPETQAILPRWRGDILPALMACADGRLDRIKLDWTPDACVCVVMA
                     AGGYPGPYDVDAPISGLEQASARDGVVVFHAGTRVSGGEAVTAGGRVLGVTALGADLR
                     AARDRAYEAAGLIRFDRAHYRRDIGARGLAR"
     gene            12703..>15100
                     /locus_tag="PK634_09510"
     CDS             12703..>15100
                     /locus_tag="PK634_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98013.1"
                     /translation="MKTNLIRSVCAAALFLSGSAFGEAYRGEMNAWGTTWMTQDTDFG
                     NIWKVTIQAASNDAPSTYKFDRYGDWVENWGAGSSATKNSTVGTLAGSGGNVSMDAIN
                     GRRYSFGMDAGHTEHVVMETDGAPVTPLVATDNHVQDPGTNPVTVSIKLSASLSTQEI
                     VWVRYATNSDFTAARVAPATGSSTNYSATLPGGHNPGARVWYYILTSTMPSNQIMSKP
                     DLCLLRGLKSGSTNFTYRPGGGMCWHHPTNGEPSGATMRNPTNSAAASAAVYFYTGNF
                     WTNGWAMGDQTGATLYHRLKGSGGGWTATNAWFDLESGNNKYWQMRIPSGTYAATNEV
                     EYYIRVTYADYDTTYLGRLTDTASRTFLVEANAQAAPFSFRYGSSTNLGNCWHLPANA
                     EPPTTTMRFPDNPYSNNAVYVYNGNQFQGSGNAADQSGGTLYHRLAGSGGGWTATNLS
                     YDSQQENNKYWVSTIPAGAYKATNQVEYYLRVTYTDRDTTYIGTTNDGAGSLTYASEA
                     SAQSNPFRYTYGGEPGTEAGYIWHNSNRVNIASGSVQFWVKIGYAQGIGSNRWVDHAC
                     LYYTTNGSAVGVAGKGTGDVNTLAQYLTFDHTEEDASENGNAMWWAGTASGLPLTGTI
                     RYKIGAWNGSGAQRFAEYNTDGVDDMEFSFSLYVSGSEGLTVNGKNADYTTTKFFVNE
                     RAGETNYVSAQYTVPGGTAIEKAEIFSNLDRRDYADTDYNGDGIPDGINPPAGGSITT
                     NDTGAYYRAYPMQFLGGRSYCWTGTASKCGAYRLTARYKLAGDTNWYWYSSGGRRDHA
                     I"
BASE COUNT         2408 a         5437 c         5141 g         2114 t
ORIGIN      
        1 acttggccga cctctcgacg gaaccggcca gcgcctccgc cgcctggtgg aacccgcgcc
       61 cggccttgaa caggtcgggc cggctcaccc gctggacacg ggccaggaag ttggaggcgg
      121 ccggggacgg gtccagcgtg tcggactccg cgatcacgcg ccggaccagg tcgccggaaa
      181 aaccggcggc ctccatgtcg cggcgcgtct cggcccggtc ttccgcgttg aaggtcccgg
      241 cgaaaaagga ggcggcgata tagtccgcgt cgccgggctc caggctcgcg cccgcgcgat
      301 ccaggcggaa ccgggcctcg gcggccgaga gaagaaaagc gtccagcgcc ggccggatat
      361 cggcaggcac ctcgtccggc cagtccggcc tcacccggga tgccgccgga tcgacgcgcg
      421 gttcccggcg gatttccatg gcgccggcca tgagcgacca gatcttcctt gaagagcccc
      481 gcgcggcggc gaagagatcg tccgccatcg gcgccagtcc gaggggccgg gacaagagac
      541 ggcgcgggcc ctcgaggacg ggatccggct tgccgtgatc tttcgcgaag gaaagatcct
      601 tcggtgtcat gttcaggtgc ttcaaggcca caaccagatg cgcggactcg tttgtcccga
      661 gaagcggcgc cggcggcgga gccgcgcacg cggcacgagc cgccatgaat ccggccaccg
      721 tggcggcgaa tccaagccgg cggcagggac cggttctcca gggatccgag aaagaagacg
      781 agggctttcc aggcattgga aattccaccg ccgtgttttt ccaacccttg gaaatgatca
      841 cgtgtttgcc cccgggcctt cgagcgctcc ggcatgcatt gttccagacg gcccgaaaaa
      901 gaacaggctc atttccaccg cccctgtcga tccattcgcg cacttcgata cgcgcgggag
      961 ttgaaaggga aggccttccg gccgatatca tcccgggccc cggcggcgat tgccggaaaa
     1021 aacagcgcgg cggcggaaat ccggcatgaa atccgcggtg tcgcgcaggt gctgaacata
     1081 gacgtgcttg aagcccaact cccccacgcg gcccgtgacc cggcgatgct cctcggccgt
     1141 caggccgcgg tccaactccc cccgcgcccc gcaagccggc acgggccgga actggctcat
     1201 gacggagatc ggcaacgaag ttccgaactc ctcccgcaac aggtccagga ccgccaggct
     1261 gttctccacg tgaccgggca ggatcaggtg gcgaaccagc acgccttgcc gggccggctc
     1321 ggatcccgtc ggatcccacg gcgccaggaa tcctttggcc ccgaccatcc gccggagcga
     1381 ctccagcgcc agccgcggat agtcctcatc tcccatgcac cggcgggcca gttccggctc
     1441 cgcgaacttg aagtccggca tgaacatatc catgaccccg gcgtactcct ccaccaggcc
     1501 gggatcctga tagccggaac tgttgaacag aaacggcacg tccgcgccgg ccgcccgaac
     1561 gcgggcgcac agctcgcgga tgtgcggcca gaagtggtcc ggcgtgacga agttcaggtt
     1621 gtggacgccc ctcggcccgg ccatggacaa cgcggcggcg agcaactcgt caaccgtcat
     1681 ctccacgccg cgatgctcga tggaaatctg gtaattctgg cagaaaaaac aaccgctgga
     1741 gcacccgctg aaaaaaatcg cgcccgaccc gcgggtcccg gaaaaggacg gctcctcgcc
     1801 gaaatgcggc ccgtacgacg ccacgcggca cgccgcggtc tcgccgcaac gccccgccac
     1861 cccggccacg cggtccacgc cgcaccggcg cgggcagagc cggcaggcgc ggtagcgccc
     1921 cggccgggcc gggtcgagct tgtctttttc ccctggaccg cgcattgttg aatcacacgg
     1981 aggagcgata caccagaccc gtccgatccg gcggcaggat aaaccatgtt tcttccccat
     2041 gccagcgtga cggagatcct ttccgcggcc gcgacgttga atgtgtcccc gggcgatacg
     2101 ctgatggtcc tggtggcgga gcacaacgcg cccgacctgg acgagttgcg gaacgccctg
     2161 cgccaaacga tcccccgctt tttcggcggc atcttccccg ccgttctgtc gggtgcgcgg
     2221 gcgcacgacg aaggggcagt gatcctggcc ctgccggcgc tggagcctcc ccgtgtatgc
     2281 gcggtctccg ccagggccgg cccgtccggc gcggacctgg ccctgccgac ggccgacccg
     2341 tgcacggcgg tcctgctggc cgacgggatg tcgccgggca tctccgagtt cctggccggc
     2401 gtgttccgcc agttgggccc cgcgacccat tgcgtgggcg gcggcgcggg cgtgtccgcc
     2461 atggtccagc gcccctgcgt cttcacggcg gaaggcgtgt tcgacaacgc ggcggtaatg
     2521 gccttcgcgc cctggcgctg ctcgctcggc gtgcagcacg gcatgaaacg gctcgtcggc
     2581 ccgatcgtcg ccacgcgcac ccgccagaac atcatcgagg aattgaactg gcggcccgcc
     2641 ctggaggtct ataaggccat cgtggaaagg gacacagggc agccgctgac ggcggagaat
     2701 ttctacgaca gcggccgggc ctgggcgtac ccgttcgggc tggtccggga aggcgcggag
     2761 gacgtggtgc gggatccgct cgccgtggtg gatcacggca tccggtgcgc gggaggcgtg
     2821 ccggagaata ccgcgctcca cgtgctcaag ggcgacgccg accacctgat cgcggccgcc
     2881 cggcgggccg gagagcgcgc cgcggcgggc gccaggagcc ggccgcgcgc cgccctgatc
     2941 tgcgactgcg tgtcgcggcg gatgttcctg aaggggcgtt tcgccgagga actgtccgcc
     3001 gtaagcgcgg ccctccggcc gatcgaccac ctcgagcccg aaggcatgct gacgatcgga
     3061 gagctgtcct cctcggggga aagcgtgctg gatttttata acaagaccat cgtggtggga
     3121 ttgctctgcg atgcctgacc cggctgccaa cgacgtcgtg gggacgattt ccctgctcta
     3181 cgagctggcc ctctcgatgg gccagtcgct ggacctggac gcgaactgcg acctgttcat
     3241 ccgcaccctg ctgacccgca agaacctggc cttcggcgcg gtctggctca aggcctccgc
     3301 gctcaagaac ggcgcgacgg ccgaagcgga ttccgggtcc taccggctgg tctacgccaa
     3361 tccccgggtt cgcgcccgcg aggatcgcct gccggccaac cacccgctcc ccgcccgtct
     3421 cgggaacatg cccttttttt ccgtcgccgc gaacgagccg ggtttcgcgg aagcggtgac
     3481 ggaaaacggg atccagggcg gcgcgtacac ggtgtatgcc ctgggcgaca tcgggttcct
     3541 caagctctac tccgtcctcc ggcggtccgc ggtttcgccg gtcgaggtca acagcctccg
     3601 gcaggtggtc gccaagttcg ccgtgtccat cgagggctgc ctcgcgcacc gccagctcgc
     3661 gcgccagatc gaggagcgcg accgggccga gggcgagcgg cggaccctgg cgatggtcgt
     3721 ggagcagatg gaggactgcg tcatcatcac ggacgccgcc ggggtgttcc gctacgtgaa
     3781 cccggccttc gagcgggtga ccggctacgc cgcgcgcgag gtcgccggcc tcacgccccg
     3841 cctgctgaag agcggccgcc acctccccga gttctatgcc gcgctgtgga agaccgtgag
     3901 ctccggccgg ccctggcgcg accggatcgt caaccggctc aagaacggct cgctggccac
     3961 gttcgacacg atcgtcgccc ccgcgcgcga cgagaagggc cggccggccg gctacgtcgc
     4021 cgtcctgcgc gacgtcacgc gcctgatgga ggtggagcag cagtacgccc acgcgcagaa
     4081 gatggaatcc atcgggcggc tggccggcgg catcgcgcac gacttcaaca acaccctgac
     4141 cgccgtgctg ggcttcggat ccctgctcct ggagaccacg cccgaagacg gccccgaccg
     4201 ccacgccgtg gaacagatcg tgctggcggg cgagcgggcc gcgaacctca cccgccagct
     4261 gctgaccttc agccggccgc ccatgccgga gatgcgggtg atggacctca acgccctggc
     4321 gctcaacgcc acccacctcc tgcgccgctc cctcggcgag gacgtggagg tcgttacgct
     4381 cctggacgag cacaccggca gcgtccgcgc cgacgtgagc ctgctgaacc agatcatcac
     4441 gaacctcgcc ctcaacgcgc gggatgccat gccccgcggc ggccggctct gcctgcgcac
     4501 cgccccggtc gagctcgacg ccgcgttctg cgccgagcac gcgggcctcg cgcccggccc
     4561 gcacatcctg ttgagcgtcc gggacacggg cacggggatg acggaggagg tccggcgccg
     4621 agcgttcgag ccgttcttca ccacgaagag gacgggcaag ggcaccggcc tcggactctc
     4681 cgtcgtgtac ggcatcgtcc gccggctcgg cgggtgcgtc gagctggaca gccggccgga
     4741 cgcggggacc gaggtgcgga tctggctgcc gcgcgtggag gcgcggggcg acacgctgcc
     4801 ggtcgaactg gaagagaagg tcgtcggcgg ccgcgagacg atcctcgtcg tcgaggacga
     4861 gcccatggtg cgcgacctgg ccacgcggct cctgcgctcc ctgggctaca gcgtgctcga
     4921 ggtcggcggc ggcgaggagg cgctttccct cctgcgccgc taccacgacc gcctggacct
     4981 cgtgctttcg gacgtggtca tgccccggat ggacggcgtg gaactgacgc tccagctgcg
     5041 gcaggagcgg ccggaactgc aggtgctcta catgtccggc ttcgcggacg acgccttcgc
     5101 ggagcgcggg ctgaacctcg atccgcgccg cctcatccga aagcccttca ccaaggaaac
     5161 cctcgcgcgg aagctccgcg agatcctgga cgaccgggga agcggcgcga acgcgtaagc
     5221 cccggaccgc gcccggtcac ggcgcccttc ccgccaggtc gggccgcagg cggaccgcct
     5281 cctcgatatg gacccgggcc tcgtccggac ggccgagttc ccgcagcgcc gccgcaaaat
     5341 tgaagtgcgc ctcggccaat ccggggtcgg cctggagcgc cgcctcgtag tgcgggaccg
     5401 cttccgccca ccggccctcc cgggccagcg cgttggccag gttgttgtgg gcgtgggcat
     5461 ggtccggacg gagccggatc gcctgcccgt acaactcctg cgcggcggcc aggtcgcccc
     5521 gcgcggcgag cagcatgccg taggcgttgc aggcgtcggg attgtccggc tccaggcgca
     5581 gcgcctcgcg gtagtgctcc tcggcctcac cgacccggcc ctggcccgcg agcagcaggg
     5641 ccaggttgac atgcgcgacc gccatgcccg gcgccacccg gagcgcctcc tcgtacccgg
     5701 ccgctgcctc ctccgcccgg ccgagcgagg ccaggagatg ggccttgttg aagcggctct
     5761 tggcgtggag cggatcctcg gccagggcgc ggtcgtacgc cgccagcgcc tccggcccgc
     5821 gccccatctt tccgagggtg ttggccaggt tgaaatgggt ccgggcgctg cgcggatgaa
     5881 tctgcagcgc cgcccggaac gattcgaccg cctcgccgaa ccggtcctgg gcggcgagtt
     5941 cgcacccgag attatcgcgc gccatccagc tggcggggtt cttcgcgatc gtgtcgcgcc
     6001 acagggtctc gatgtcgcgg taggcatggg cctgctgccg ggtaagcgcg gccagcaaca
     6061 ggatcggcaa cgccgcgagg gcgcggcggc tccaggcggg gagcccgacg cggcgcgcca
     6121 ggcccgcggc cccggcggtc gcgagcgcga tgggcccgag gcaggccagg tactgatagt
     6181 gatccgcgac gaacgaatac tggaacgtgt agagcgggat gaacccgagc agcggcgaca
     6241 ggcaggcgac gaagaagagg gccgccagcg ccggcccgcg gattccccgc cgccacagaa
     6301 gcgccagccc gaccgccgcc gcgaggcccg ccagcagcca gccatactgg cggggatcgg
     6361 aggcatcgat ggcccaccgc gaatagctga actccaggcg gaccggccag agcagcttcc
     6421 cagcgtagaa ccagagcgca cggctggcga tgagcacgca gtcgaggaac ggaaaacgca
     6481 cccccgcgtc ccggctgccc tggaggtgct gctcccacca cgcggtgaag aggcccatgg
     6541 ccaggccgat ccccaacagc gccgccacct gcagccggcg cgcgggcgtc aggcggattc
     6601 ccttgcgcca ggcggcgagg accagcgcgg ccggcagcgt gcaggccgtg gtcttcgcga
     6661 acagggccag caggtagaac gcgaagaccc cggccccgaa ccaacggcgc cgccggccgt
     6721 ccacctccag cgaccggatc cacgcccaga gggcggccag gtagaacagc gtcgagagca
     6781 ggttcttctg ctcggtgatc cacgcgacgc tttcgacctg gacgggatgc agcgcgaaga
     6841 gcgcggccgc gagccaggcc ccgggcgccg cgagcaggcc gagcacgagg gccgcgagca
     6901 gcgcgttggc cccgtgcagg ccgacgctga ccaggtgata accgagcgga tccaggccca
     6961 aggccgaccg ctcggcccgc atcagggtgt acgtgagcgg gaaatactgc gagggggcgt
     7021 cggtggaaaa ccagatccgc cgcaggccgt cgggcgccgt gagcagctcg ttgcccgcca
     7081 cgtagacatc gtcgtcccag agaaagccgc cccgaaggga cggcccgtaa gccgccagca
     7141 gcaggcccag cagaaggagg gcccccgcga gccggacctt cgcggcgcgg ctcacggcac
     7201 cgggaaccgc gccgtcagcg cgaccacgtc ggcgcggacc gcctgcagcg ccccggcgtc
     7261 ctccggcgcg gacaggacgc ggccgatgaa ctccgcaatc ttccccatct cgggttcttt
     7321 catgccccgc gtggtcacgg cgggcgtgcc gacccggatg ccggaggtga cgaacgggct
     7381 ctgcttgtcg aacgggatgg cgttcttgtt gaccgtgatg caggccttgt ccagcgccgt
     7441 cgcggcgtcc ttgcccgtga cgcccatcgg cgtgagatcc acgagcatca tgtggttatc
     7501 cgtgccgccg gagacgatcc gcagcccgcg ctgctcgagc gcggcggcca ggacctgcgc
     7561 gttgcgcacg atctgctgct ggtaggtctt gaacgcgggc tggagcgcct cgtggaagca
     7621 cacggccttc gcggcgatca cgtgcatcag cggcccgccc tgtgtcccgg ggaagatctg
     7681 ccggtcaatg tcctgggcga accgctcgcg gcacaggatc atgccgccgc gcgggccgcg
     7741 cagggtcttg tgcgtggtcg tggtgatgaa atcgctgaac gggacggggt tcgggtggca
     7801 gcccgcggcg accaggccgg cgatgtgggc catgtccacc atcaggaacg ccccggcggc
     7861 gtcggcgatc tcgcgcagcc gcttgaagtc gatgatccgc gagtaggcgc tggccccggc
     7921 gcagatcatc ttcggcttgt gctccttcgc cagcgccgcg atctcgtcgt agtcgatccg
     7981 ctcggtctgc gggtccacgc cgtagtggac cacgttgaag aagcggccgg agaagttgac
     8041 cttgtggccg tgggtcaggt gcccgccgca cgcgaggctc atggccagca tcgtgtcgcc
     8101 gggctggagc atggcgaagt agacggccat gttcgccccg ctgccgcagt gcggctggac
     8161 gtttgcgtgc tcggcgccga agagctgctt cgcgcggtcg atcgcgaggc gctcggcctc
     8221 gtccacgaac tcgcagccgt tgtaccagcg cttgccgggg tagccctcgg cgtacttgtt
     8281 ggtcatgatc gagccctgcg ccgcgcggac ggccgggctg acgtagtttt ccgacgcgat
     8341 cagctccagg ttctcccgct gccgccgcgc ctccttgcgc agcgcggcgt agagctccgg
     8401 gtccacggcg ttcacggcca cgggatcgga ctggagcgcc tcgatcgccg ccatcttccg
     8461 cacgcgccgc tcgtgccggc tcccgacctc gaactcggcg gacagccacg cgtccagcat
     8521 cgacgccgcg tcggcgggct tcagcaggct gccgccgagg acgaggatgt tcgcgttgtt
     8581 atggacgcgc gccagccgcg ccatcgccgg cgtgaagcac agggcggccc gcgcgccggg
     8641 gaacttgttg gccgtgatgc tcatgcccac gcccgtggcg cagaccagga tcccctggtc
     8701 cacggaaccc tcggacaccc gccgggccac ggcggcggcg tagtcggggt agtccaccga
     8761 ctccgcgctc ggggcgccga cgtcctccgc ccggatgttc cgctccgcca ggagtttctt
     8821 cagggcctcc ttcagctcga agcccgcgtg atccgaaccg atcgctattt tcatgcccgc
     8881 tcccttcggc gccgccggcc gcacgccgcg ctgctcgacc aggtacagaa ccaaatccga
     8941 aatcgcgctg tcaatctgct cgctcgtccg ctggtacacc cccacgggcg acccgatcgg
     9001 gtcggggatg tcccgctccc cgccccccgc gccgaacgac gtcagcagcc gcaccttgcc
     9061 ggacgccccc gggaacgccc gggcgaccgc ctggcggtgc gcctccgtca tcacgacgat
     9121 cagcgtggcc gcccggacca gctccgcggt cagcatccgg ctccggaacg ccttcaggtc
     9181 cagcccgcgc cgcgccatca cctcgacggc ctccgggctg gccggcgcgc cgttcggcgc
     9241 ggccacgccg gcggacccga ccgtgacgcc cgccgccggc ccgagccggg cgcgcagcag
     9301 gcgctcggcc atcgggctcc ggcagatgtt gccggtgcag acgaacagca catgctccgt
     9361 ggaatctccg ctcatgccag gatctccgcg cgggcgccgg cgagcggcgc ccctacggct
     9421 ccttctccgg cggcggctta ccgcgcacgt tgagcgcctt cagggccagc aggatatccg
     9481 cggcccggcc cagcgcggga tcgccccgca cgcgggcgcg cagcacgcga tgctccttct
     9541 cctccgccag caaccggccg ttgcgcgagt ccggctccgg ctcgtactcg tcgcccggaa
     9601 cgccctcgac ggtcacgtcg ggctcgaccg ccgtccggcc gttcagcacc agcccgtccg
     9661 ccatcaccag ctggcgcgtc gcaaggtaca ggtatccgcc gcccgggcag gataccacct
     9721 cgcggacggc cggatcgccc cgcgtcgcgg acccgatcag catggccccg cgcaggctgt
     9781 cggcgaagac cgccgccagc gcctcgcaag cgccttccgt ttcgccgtcg atcaggacca
     9841 tcaccggcgc atcgagcccc gacccgccgg gggcgcggaa cgcctcgatg tcctgtccct
     9901 tcgccatgcg cagggaaaag agaagcgcgc cctccggcgc gaacaggcgg gcggcatccg
     9961 cggccgccgc caagttggtg cccccggcgc cccggagatc cacgatgatg ccacagtagt
    10021 tggtcccgtc ccagccacgc agcgccggga ttacttcctt cgaggccccg tcgtacaggc
    10081 cgttcaggcg gagataaccg atccccgtgg ggagatcctc ggcgaccgcg acggagggct
    10141 gcgggatgcg ggccgcggcc agttccaccg actgggttgc cgcgccgcgc aggaactgga
    10201 cgcggaaccg gctgtccggc ccgccccgca gcagcgagcg gatttcccaa tccttcaggc
    10261 ttgccgccgc tccgccgtcg atgaactcca ggcgatcgcc gggctgaagg ccggcggcct
    10321 cggcgggcga accggcgacc acttccgcga ccttcggcat cccgtcggca gaccggatgc
    10381 gcaggcccgt ttcgaaaacg aagccctgca agcggtcctc gagggccgac ttctcggcct
    10441 cgttcagcca cagcccgccg ggatcggcgc tcttcaccat ggcggagacc gcggcgcgcc
    10501 gggccgaatc cgcgtccagc gtcaacccat aggattcgag cagcgcggcc aactgttcca
    10561 cggcgggcac ggcatcgcgt ttcagggagg gcggcgcctc gggcggggcc gccggcgccg
    10621 gggctgtctc cgccgcgacg gcggcggggg cgggcgaggt cgccgggtcc ggcgcggccg
    10681 gttcctgggc ccaggcccgg cccgccgcga atcccgcgcc cagcacgaaa gccagtatcg
    10741 atttcatgtc gtcttctccg cggccgcttt cagccgccgg ttcccggccg ccactttctt
    10801 cttcatcgtc cgcttgtgcg cctccagccg ccgccgcaga tccggccggc ccagcgccag
    10861 gatctgcacc gccagcaggg cggcgttcac cgcgcccgcc ttgccgaggg ccacggtggc
    10921 cacgggtacg cccgagggca tctggaccgt cgccaggagg gcgtcgaggc cgtccagcgc
    10981 gcccccgctc accgggatgc cgatgaccgg aaggatggtg ttggccgcca ccgccccggc
    11041 caggtgggcc gcgccgccgg cggctgcgac gagcaccttg agcccgcggc gggcggcgga
    11101 acgggcatag gcggcggccg cgtccggggt ccggtgcgcg gagagcacgc gcgcctcgaa
    11161 cccgacgccg aactcgcgca aggtcttcgc cgcatgctcg acgacgggcc agtccgaatc
    11221 gctgcccatc agcaccgcga cctctgtttt cgcttttctc atcgtgccaa tcctcgcgcg
    11281 ccaatatccc ggcgatagtg ggcccggtcg aaccggatga ggcccgccgc ctcgtaggcc
    11341 cggtcccgcg ccgcgcgcag gtccgcgccc agggcggtaa cgcccagcac gcggccgccg
    11401 gccgtaaccg cttccccgcc ggaaacgcgg gtgccggcat ggaataccac aaccccgtcc
    11461 cgcgcggaag cctgttccag cccggaaatc ggggcgtcca cgtcatacgg gccggggtat
    11521 ccgcccgccg ccatgaccac gcacacgcag gcgtccggcg tccaatccag cttgatgcgg
    11581 tccagccggc cgtcggcaca ggccatgagc gccggcagga tatcgccccg ccaacgcggc
    11641 aggatggcct gggtttccgg gtcgccgaac cggcagttga attccagcac cttcggcccg
    11701 cctgtcgtca gcatgagccc ggcataaagc actccgcgat aagttattcc gcggcgagaa
    11761 agctcgccga tcgttcgctc gaaaacgtcg cgccgcacag cctgccactg ggccggggtc
    11821 aggacagggg cgggcgagta ggcgcccatg ccgccggtat tcggccccgt gtcgccgtcc
    11881 cccacccgct tatggtcctg ggcggaggcc agcatttcaa tatgggatcc gtcgacaagg
    11941 gccaggacgg agacctcttc gccctcgagc cgctcctcga tcagcacttc gcggcccgct
    12001 tcgccgaacg cgcggttgac cagggcgtcc tcgacggcct tttcggcctc gtcgggcgtg
    12061 tcgcagacgg tgacgccctt gccggccgcg aggccgtcgg ccttgaccac gacccggccg
    12121 ggctgactcc gaatatagga aagggcctgg ttggcgtcgg tgaaccgcgc ggcgcgggcg
    12181 gtcgggacgc cagctttttc catgatttcc ttggcgaagg ccttgctgcc ctccagccgg
    12241 gcggcggagc ggccgggccc gaagacccgg agcccgacgg cctgcaggcg gtccgccagc
    12301 ccggcgcaga gcggcgcttc cgggccgatg accgtcaggt cggggcgcgc cgcccgcgcc
    12361 cacgcgacca ggctatcgag gtcggaagcc gggatcatca ggttttcagc gagccgggcc
    12421 gtcccggcgt tgccgggggc gcagaagagc tccgggcggc aggaatccct cgagattttc
    12481 cacgccagcg cgtgttcccg tccgccgccg cccacgatga ggatacgcat gcccgccata
    12541 cggccccggg accatacagg acgggcggcg ggcagaccag cctgatttca ggacacggcc
    12601 cccctcctcc ccttggccgg ctcttgaagg tggacatcat tccgggtacc tgttactaaa
    12661 cgctgaatca cttcaggcgg tgcgcccgat aaaggagtgc tcgtgaaaac caacttgatt
    12721 cgatccgtgt gcgcggcggc gctttttctc tcgggctcgg cgttcggcga ggcgtaccgc
    12781 ggcgagatga acgcctgggg aacaacctgg atgacccagg atacggattt cggcaacata
    12841 tggaaggtga ccatccaggc cgcgtccaac gatgctccct cgacgtacaa gtttgatcgc
    12901 tatggcgact gggtcgagaa ctggggggcc ggcagcagcg ccacgaagaa ctcaaccgtc
    12961 gggaccctgg cgggttccgg cggcaatgtg agcatggacg caatcaacgg ccgacgctac
    13021 agcttcggca tggacgccgg ccataccgaa catgtggtga tggagaccga cggggcgccc
    13081 gtcacgccgc tcgtggccac ggacaaccac gtccaggacc cggggaccaa cccggtgacg
    13141 gtgagcatca agctctcggc gtcgttgagc acgcaggaaa tcgtctgggt gcgctacgcg
    13201 acgaactccg acttcaccgc cgcgcgggtc gccccggcga caggcagttc cacgaactac
    13261 agcgccacat tgcccggcgg gcacaatccc ggcgcgcggg tctggtacta catcctgacc
    13321 tcgaccatgc cgtccaacca gatcatgagc aagccggacc tgtgtctttt gcggggtctg
    13381 aagagcggga gcacgaactt cacgtaccgg ccgggcggcg gcatgtgctg gcaccacccg
    13441 accaacggtg aaccctccgg cgcgacgatg cgcaatccga cgaacagcgc ggcggcctcg
    13501 gccgccgtgt atttctatac cggcaatttc tggaccaacg gctgggcgat gggcgaccag
    13561 accggcgcga cgctgtacca ccggctcaag ggctcgggcg gcggctggac ggcgaccaac
    13621 gcctggtttg atttggaaag cgggaacaac aagtactggc agatgcggat ccccagcggg
    13681 acgtacgccg cgaccaacga ggtggagtac tacatccggg tcacgtacgc cgactatgac
    13741 acgacctacc tcggccggct gacggacacg gcctcgcgga cgttcctggt cgaggccaac
    13801 gcgcaggccg cgccgttcag cttccgctac ggctcgagca cgaacctcgg caactgctgg
    13861 catctcccgg ccaacgcgga gccgccgacc acgacgatgc gcttcccgga caatccgtac
    13921 tcgaacaacg cggtctacgt gtacaacggc aaccagttcc agggctccgg caacgcggcg
    13981 gaccagagcg gcggcacgct ctaccaccgg ctcgccggca gcggcggggg ctggaccgca
    14041 accaacctct cgtacgacag ccagcaggag aacaacaagt actgggtctc cacgatcccg
    14101 gccggcgcgt acaaggcgac gaaccaggtg gaatactacc tccgggtcac gtacaccgac
    14161 cgcgacacga cctacatcgg cacgaccaac gacggcgcgg ggagcctgac ctacgcgtcc
    14221 gaggcctcgg cgcagtccaa cccgttccgc tacacgtacg gcggcgagcc cggcacggag
    14281 gcggggtaca tctggcataa ctccaaccgg gtgaacatcg cgtccggctc ggtccagttc
    14341 tgggtcaaga tcggctacgc gcagggcatc ggcagcaacc gctgggtgga ccacgcctgc
    14401 ctttattaca cgaccaacgg gtccgccgtg ggcgtggcgg gcaagggcac gggcgacgtg
    14461 aacacgctgg cgcaatacct gaccttcgac cataccgagg aggatgcgtc cgagaacggc
    14521 aacgcgatgt ggtgggcggg cacggccagt ggcctgccgc tgacgggcac gatccgctac
    14581 aagatcggcg cctggaacgg ctcgggcgcg cagcgcttcg cggagtataa caccgacggc
    14641 gtggacgaca tggagttcag cttctcgctc tacgtgtccg gctccgaggg cctgacggtg
    14701 aacggcaaga acgcggacta cacgacgacc aagttcttcg tgaacgagcg cgcgggcgag
    14761 acgaactacg tgtccgccca gtacaccgtc cccggcggca cggcgatcga gaaggcggag
    14821 atcttctcca acctcgaccg gcgcgactac gcggacacgg actacaacgg cgacggcatc
    14881 ccggacggca tcaacccgcc ggcgggcggc agcatcacga ccaacgacac gggcgcgtac
    14941 taccgggcct acccgatgca gttcctcggc ggccggtcgt actgctggac cggcacggcg
    15001 agcaagtgcg gcgcgtaccg cctgacggcg cgctacaagc tcgccggcga cacgaactgg
    15061 tactggtaca gcagcggcgg ccggcgcgac cacgcgatcg
//
LOCUS       DAOOTK010000080        15097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2677_length_15097_cov_4.391570, whole genome shotgun sequence.
ACCESSION   DAOOTK010000080 DAOOTK010000000
VERSION     DAOOTK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_15097_cov_4.391570"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1782)
                     /locus_tag="PK634_09515"
     CDS             complement(46..1782)
                     /locus_tag="PK634_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98014.1"
                     /translation="MFNPRRDRPGARAWIVAACSLAVAAAVRAQEPAKGMLILPPGPG
                     AGPYAERPPVEFGVGDQQALAEGLQGPDGLAIHPQSGEIYFSEEDAARVWVLRDGRPM
                     RVADRDTPLYRLRDGRRTADPVMPLRNPEGLAFSSTGELYVVEDYPGGRLIRFPPRAD
                     GRYPEGEVIEFPGDWRTFAWEGVAVGPENDLLMAGSDVEYALGGGGAKPFMGAILYRD
                     SVGQWWVPYRRPFASFSSVQFTRGGRQAVYTCEVSGEVGWLDLQGRRPIGGCSSAAVK
                     SPEGIAVLPDGRLVVALETGALVVVDPAMDQHATVAEGFGSLESVQWDAFSNRLVLTE
                     QSRGRVLSLKARPDFQAEENRMDMAVYHPLFNPRHVPEVCPPYLARILAFGGLDYTRP
                     GLPPISFRDFAQRVPMIAADARAAPVENQEPVEDPIERVQFVVFKPNQMVMSETGPQP
                     ALALFATRTQSGRVTATSLLRAETMGISLDRPKTERIGTAALAVPMPAGVGVSSLGVA
                     HINFMGLGKTPDYSVVLNPGQPFDSYLVVYETGGRRVHYKLEFSEEGAGLDSWVVAFT
                     ESRPDEWLALGR"
     gene            1868..2428
                     /gene="tadA"
                     /locus_tag="PK634_09520"
     CDS             1868..2428
                     /gene="tadA"
                     /locus_tag="PK634_09520"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOW98015.1"
                     /translation="MPPEIDNRTPASPHERFMRLALRQARAAADLEEVPVGAVIVREG
                     QLIAQAHNQVELLKDPTAHAEILAITQAAAALQDWRLAGTILYVTKEPCPMCAGAIVL
                     ARIPRVVWGVADPARGGAVSLFPIFQNPRLNHRVEFEGGVLEEECRGLLQDFFRKHRA
                     DRAAGSAGASRGGAGADPADGRPAPT"
     gene            complement(2331..3968)
                     /locus_tag="PK634_09525"
     CDS             complement(2331..3968)
                     /locus_tag="PK634_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98016.1"
                     /translation="MTGINQLHRWALHRLLRWLLPLVMLIGVLAALLVERRAAGNSVF
                     VEDRAYADMAVARTLVLDGSYGLQPNAPCPAIRDTLWRLALAAAGWLVGSVRAAALLL
                     GTVCAIGTLLLVLRLARLLFPFPPFILYSAVLIIAAPGLLTHAISGTSRALATLLATA
                     ACLLHVEGMRDRRPVLSLGCAVLVGLLAWIRLEFVLLWIVFWAQAVLRITPEGPGKGR
                     RQAALVKGATGLLLMAILLLPLFAWNLHWLGVAWPQAAGAPLTLNAWADRPLGETAGR
                     SLVLSLQALPLLWGPVSGVPLLQPFLGRLLVWFGAGLIAVLAVRRGEEYPYLLVPLLL
                     LLLPVGLAILRPYLGADSSAVVMGALGPLFLLTAAFGIFRLPFALENLYRKWKAGLPE
                     ATGFDIWWTAVGGLLMLWALASMGRFAARAAAERSDAVQARQYVSDALVSGRARAARI
                     LTDAPGWLAFTHAVPVLDLGGEFTPGLLPALRPDGRWNPDGLAEFLLEQPADLLILWK
                     AGLEDVTSALGGRPLDPPPRRHGTLPLIRPLDPPGAF"
     gene            complement(4006..4929)
                     /locus_tag="PK634_09530"
     CDS             complement(4006..4929)
                     /locus_tag="PK634_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOW98017.1"
                     /translation="MKRVVALGMAGWLAAGCACAESVRVVGQRVNLRAQADLKAEVVG
                     QVADGEILEARSIGDAWVEVAPPDSVDLWVHKDFVQGGKVVATELKVRGGPGINYRVV
                     ATLPRGAEVKVRGEFTDWLKIAPPPGASLWVSRSYVEPVGASRPPAAESAAAPAGADA
                     PADSAPTVAASSIPPPPPVSPSAPPPPAPAAGTMASQRVVRVQTGREPVVKPPPAGLV
                     LIPFEGQGRSVQQDGVLKHAGNLLGRPSRFRLVNPGGNQLGTLCYVYGNNAQLETFVG
                     RRLLIRGREYWVRGVQYPVVVPDQIIPKAGP"
     gene            5054..7237
                     /locus_tag="PK634_09535"
     CDS             5054..7237
                     /locus_tag="PK634_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98018.1"
                     /translation="MNHPGANESAAGRWASGWELGPPAAARGEPSSARLRTMNRPGSV
                     GPDAQPPGWSFHLEWPEERGPVTPELFACGYRLRFARAPESACAVFLAEPGLLCGHLG
                     ARVAPALFCAETDIQTAGPMTWIEREHQHVVLVQEAAGDGLRFCLAVETRAGHAEAAE
                     RARRGLERKFEDQFQAELAQRPTRGAPTEPRALDPAVAHLAIETLFARLRPPQGLLPF
                     RWVEQDPSGPARFSLRHLYPLVFAWTPIDPAVALDLVKCALSCQQPDGRIPSEVSPDY
                     AGEQSPAALPLIAQAARQAWSAHRDPGFPDFAAPRLRRYLRWACRRYDPEQTGLPCWP
                     SADEALIPEAHDAGLASPDLAAFLLCEIDAYLQLARACVTTAGDEATWQAEIQRLKDA
                     LHQTLWDERRSVFTSRYVRGAVIERLTLSSLLPLPAPGLRADQRQAILQGLRDSAGFR
                     GPAGAPAWERWEGDAEPPPTHALHQILLLEALRRGNAPDEARALSHTLCRTLAGLDFD
                     GGSLPDRLDAPPQKPGIIGTAAAALAVILDSPVAVEPAAPASPALAWMERHRASLLAG
                     VVGLLVLGLATVAVAFLSKTAPPQATLSAWTGLATRYHQEGRYDEAQELYEKILASGR
                     NLPLVELLLANTLLRKGELEEAEERYRKLVAAPDPHPVACLNLAVTLYLRGQTNEAFL
                     TFREFADKYGELQPQLAFRARTAMQLLAPDEDAAAVTGLPAGPGE"
     gene            7436..7777
                     /locus_tag="PK634_09540"
     CDS             7436..7777
                     /locus_tag="PK634_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOW98019.1"
                     /translation="MKTRHFCIGLFLAVTAFLAAASGCYRRDIRTLEVKVPQMGSPEC
                     ARIVETVFNKMDGIEQVDIDLANRRILVKYDGLKMGIVNIEYLLTNAGFDANDKPARP
                     EARAKLPPECR"
     gene            7782..9278
                     /locus_tag="PK634_09545"
     CDS             7782..9278
                     /locus_tag="PK634_09545"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOW98020.1"
                     /translation="MKTRLVSVPLARQPADGWLLFAFQDEPVPVPGSDPVSRRLDRML
                     KDWRARHDFIGKLNQVAVISSWESLPARSIVLAGLGKKKDFHVARFRQAAAAAAHTLR
                     KHRLATLAVSLRAPDLPLAPELLAGHLAAALHYGHYAFTRYLTKDEDGPDRRMPDILF
                     TDAPVRAPALARRLADTVAASEILKEVRDLANLPANEAAPAALAGAARALARKHGLAC
                     RVMDKAALQRKGCGALLAVGRGSRHEPRLIILRYPGTDRKLRPVALVGKTITFDTGGI
                     SIKPGKGMEWMKYDKCGGMAVLAAVLMAARLRLPRPVIALLPAAENMPGGDATRPGDI
                     IRSYSGQTIEILNTDAEGRLLLADALSLAAEYKPAAIVDLATLTGASVVALGHVLSAV
                     MGNRPEIVEQLRQAGETSGERLWPLPLLPEYDEDIKGAFSDLKNVGERGAGTIIGGTF
                     LRHFVPESIPWAHLDIANTAYEESTKPYAPAGPTLFGARLLVEWLRNL"
     gene            9376..10266
                     /locus_tag="PK634_09550"
     CDS             9376..10266
                     /locus_tag="PK634_09550"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOW98021.1"
                     /translation="MTTLTQLDLPGIPKLRSGKVREVFDLGDRLLMVATDRISAFDCI
                     LPTGIPQKGEVLNRLSAWWFERTAAIVPNHVIALDPSVFPERLRPFEHLLRGRAMLVR
                     KAKPLPVECVVRGYLIGSGWKDYTRTGSVCGLPLRAGCRLADRLDEPLFTPARKADTG
                     HDENIPFGEVEKMIGPELAARLRAVSLALYEHAAGHARTRGIIIADTKFEFGLDGNEL
                     ILIDELFTPDSSRFWPADSYAPGRSPPSFDKQFVRDYLETLDWDKTPPAPPLSDDVVR
                     KTREKYLEAYRRITEREDIR"
     gene            complement(10294..11505)
                     /locus_tag="PK634_09555"
     CDS             complement(10294..11505)
                     /locus_tag="PK634_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98022.1"
                     /translation="MRGVIRTAVVVCILLCCVMASAESYSSRPMAADGAQLIEDGTWC
                     ARASDERMAAATDWGEGEWFQPMATCPRVHLSEIKQRASYLIGQYINELAMSACYGAM
                     IPYSYPSNIFYYTVTGQLARYDLPADYLVNTPFNILETNIYGYQGAKWLFQDMQWTAG
                     EGGVIGKRRRHDRIGEVSAPGEYPPIDGGPPAHACNNLCVDTNSVDEYESYMISTSAT
                     IGDGGGEFMDYCAYGLGYTNGYVTYHWWGYSLTGIESGARIGMLIPYSRSPTSSYASL
                     LLRPHRLCVYRQSGGGDSNTWAFLGQPYFEDTNHVLHADGTVSISSIETAEGSMPVGE
                     MSAYAVPLIDVFSLACQSNLWYWIGDPDADNMTTACSETPVYGWSAWALRANTQSVSY
                     RHFVRWNFAFE"
     gene            complement(12110..13378)
                     /locus_tag="PK634_09560"
     CDS             complement(12110..13378)
                     /locus_tag="PK634_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98023.1"
                     /translation="MKIGIICLAVMLAGLVPSFGDESRPDSRALIAAVVQGLGEKAIN
                     AFPDPSPILWLLETNPDFRALCAASDSTWQQTFSALVDSSLDRASRLVLLHSYLYLSP
                     KEYANCLDRVLALHMQGILPQEELLNIFLFPPRDENRWFLSYNADDPRVKKFLGEVRV
                     AFANNTDVLHMIDIITSGKARARDERLRSGKSSAFKRQIPSLAGEDIHASDHDKQKGA
                     IGVSPQITNNANESVEALIASARAGSREALLELGDRGNPDVIPFLKAIMSVNSNKSFG
                     GAGACAQMALVKLGDTEALAEIAAELEAVSTPWVQDQAIQKLEYVANSNAVGLLLMLL
                     TNNAERSETMKEEPRGERPLDRLIYEPLNVVAMRSLAKIIPNPVVWKGPTPTEDDIAR
                     WREWWARKEWPSRDTALFPYIELQPSTKHK"
     gene            complement(13390..>15097)
                     /locus_tag="PK634_09565"
     CDS             complement(13390..>15097)
                     /locus_tag="PK634_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98024.1"
                     /translation="NAWSSCPSNQPGKIYDNVGWLKLTHEAYSNWGGYIWCLQNTIAF
                     TSSASSIYVSPAGATSEIASVLVGTLGDFCPKTVVTDVGPVFNTNYPGSGMATEYVAR
                     ADLAAYYKLVSSNYLAVNDDDSDGDGIPDFADGYDRDGQGNSSDDLSTNDFFVPWPVL
                     LSGYTEPAQAMVSISYNASDPSGVTLGTNGYVPASGYFRLWRTNAAQARNSASILSGG
                     DYIPPGTYPAASLGFITKPQLVDFYIEPVVPVLNQPLVFEVDPDGPGPKGAVCVDKLI
                     CSVLMVESVEIVGAPADGLVVENGDDVTFVAHILPSDFVPPADEPKWYYQKLDADGSW
                     ESWQSFGANADGTQYVHTATESGIFRVETVLTVGGVACEKIYERTADDPHSTLKEGEP
                     DAFGVVDNAIQLAVREEALGYIGSTYYSSAGSLPAWRDPFGLFDGQNKCNVFVADVCE
                     GSGADVDPPTAGVYLGGPPSANNWAGMPDINDPNAPYPIPNWTLLFDNASPQPGHVCA
                     SGYEISGSSGHAGIVDYDGQWISASSAVGGNVNRKAEFSSYRRHYQSGATKPAGQRAY
                     SN"
BASE COUNT         2738 a         5218 c         4692 g         2449 t
ORIGIN      
        1 cgcccagccc gatttccgcg catagcgaca gccttccgtc tccccttacc ggccgagggc
       61 gagccattca tcgggtcgcg actcggtaaa ggccacgacc caggagtcga ggcccgcccc
      121 ttcctccgag aactccaact tataatgtac acgccgaccg ccggtctcgt ataccacgag
      181 ataactgtca aaaggctggc cgggattgag cacgaccgag taatccgggg tcttgcccag
      241 tcccatgaag ttgatgtgcg ccacgcccag cgacgacacg cccacgcccg ccggcatcgg
      301 cacggccagg gcggcggtcc cgatccgctc ggtcttcggg cggtccaggc tgatccccat
      361 cgtctccgcc cgcaggagac tggtggccgt cacccggccg gattgcgtgc gcgtggcgaa
      421 cagcgccagc gccggctgcg gccccgtctc gctcatgacc atctggttgg gcttgaacac
      481 gacaaactgg acgcgctcga tcgggtcctc gacgggttcc tggttttcca cgggcgccgc
      541 ccgcgcgtcc gccgcgatca tcggcacccg ctgcgcgaaa tcgcggaagg agatcggcgg
      601 caggcccggc cgggtgtagt ccagcccgcc gaaggcgagg atgcgggcca ggtacggggg
      661 acagacctcg gggacatggc gcggattgaa cagcgggtga tacaccgcca tgtccatccg
      721 gttttcctcc gcctggaaat ccggccgggc cttgagcgaa agcacgcggc cccgggactg
      781 ctccgtcagc accagccggt tggaaaaggc gtcccactgc accgactcga gcgagccgaa
      841 tccctccgcc accgtcgcat gctggtccat cgcgggatcc accacgacca gggcgcccgt
      901 ttcgagcgcg accacgagcc ggccgtccgg caggaccgcg atgccctccg gcgacttgac
      961 ggccgcggaa ctgcacccgc cgatgggccg acggccctgc aggtcgagcc agccgacctc
     1021 gcccgacact tcgcacgtgt acacggcctg ccggccgccc cgcgtgaact gcaccgagga
     1081 aaaactggca aacggccgcc ggtacggcac ccaccactgc ccgacgctgt cgcggtagag
     1141 gatcgcgccc atgaacggct tggccccgcc gccgcccagc gcgtactcga cgtccgagcc
     1201 ggccatcagg aggtcgttct cggggccgac ggccacgccc tcccaggcga acgtccgcca
     1261 gtcccccggg aactcgatca cctcgccctc cgggtaacgc ccgtccgccc ggggcggaaa
     1321 acggatcagg cgcccgccgg ggtagtcctc gaccacgtac agctcgccgg tcgaggaaaa
     1381 cgcgaggcct tcgggattgc gcaacggcat gaccgggtcg gccgtgcgtc ggccgtcgcg
     1441 caaccggtag agcggcgtat cccggtccgc cacccgcatc ggccggccgt cgcgcaagac
     1501 ccacacgcgc gcggcgtcct cctcggaaaa gtagatctcg cccgactgcg gatggatcgc
     1561 cagcccgtcg ggcccctgca atccttccgc cagcgcctgc tggtccccca cgccgaactc
     1621 gacgggaggg cgctccgcgt agggccccgc gccggggccg ggcggcagga tgagcatgcc
     1681 cttcgcgggc tcctgcgccc gcacggcggc ggcaacggcc aggctgcagg ctgcgacgat
     1741 ccaggccctt gcgccgggcc ggtctctcct cggattgaac atacctttat actaaccata
     1801 ccgctttcag gcttgagcgt taaaaagcct tgcgtcacat tcgcgccggt caccggaagg
     1861 aaagcccatg ccgcccgaga tcgacaaccg cacgccggcc tcgccccacg agcgtttcat
     1921 gcgcttggcg ctgcggcagg cgcgcgccgc ggcggacctc gaggaagtgc ccgtcggcgc
     1981 cgtgatcgtt cgcgagggac aactgatcgc gcaggcgcac aaccaggtgg aactgctgaa
     2041 ggacccgacc gcgcacgcgg agatcctggc gatcacccag gcggccgccg cgctccagga
     2101 ctggcgcctg gccgggacga tcctgtacgt gacgaaggaa ccctgcccca tgtgcgcggg
     2161 cgccatcgtc ctggcgcgca tcccgcgtgt cgtgtggggc gtcgccgacc cggcccgggg
     2221 cggggccgtt tccctcttcc cgattttcca gaacccgcgc ctgaatcacc gcgtcgagtt
     2281 cgagggcggc gtgctcgagg aggaatgtcg aggcctgctg caggatttct tcagaaagca
     2341 ccgggcggat cgagcggccg gatcagcggg agcgtcccgt ggcggcgcgg gggcggatcc
     2401 agcggacggc cgcccagcgc cgacgtgacg tcctccaggc ccgccttcca caggatcagc
     2461 aggtcggccg gctgttccag caggaactcc gcgaggccgt cgggattcca ccggccgtcc
     2521 gggcgcaggg ccggcaggag gccgggggtg aactcgccgc cgagatcgag cacgggaacc
     2581 gcgtgcgtga acgccagcca gcccggcgcg tcggtcagta tccgcgccgc gcgcgcccgt
     2641 cccgaaacga gcgcgtccga aacgtactgc cgggcttgca cggcgtccga ccgctccgcg
     2701 gccgcgcgcg cggcgaagcg ccccatgctc gcgagggccc agagcatcag cagtcctccg
     2761 accgccgtcc accagatgtc gaagcccgtc gcctcgggca gcccggcctt ccacttgcga
     2821 tagaggttct cgagggcgaa cggcaggcgg aagatgccga acgcggcggt cagcaggaac
     2881 agcgggccca gcgcgcccat caccaccgcg gacgaatccg cgcccaggta cgggcggagg
     2941 atcgccaggc cgacgggcaa caggagcagc agcaggggca cgagcaggta gggatactcc
     3001 tcgccgcgcc gcacggccag caccgcgatg agcccggcgc cgaaccagac cagcagccgg
     3061 cccaggaacg gctgcagcag cgggacgccg gacaccgggc cccacaacaa cgggagggct
     3121 tggagcgaaa ggaccaggct gcgtcccgcc gtctcgccca gcggccggtc ggcccacgcg
     3181 ttcagggtca gcggcgcgcc ggccgcctgc ggccaggcga cgcccagcca gtgcaggttc
     3241 cacgcgaaca gcggcaacag caggatggcc atcagcagca ggccggtggc gcccttgacc
     3301 agcgccgcct ggcggcgccc tttcccgggg ccctccggcg tgatccggag caccgcctgc
     3361 gcccagaaca cgatccagag cagaacgaac tccagacgga tccaggcgag cagccccacc
     3421 agcaccgcgc agcccagcga caggacgggc cgccggtcgc gcatcccctc gacgtgcagc
     3481 aggcaggccg ccgtggccag cagcgtcgcc agggcccgcg acgtgcccga gatcgcatgg
     3541 gtcagcaggc cgggggccgc gatgatcagc acggcggaat agaggatgaa cggcgggaac
     3601 gggaacagca gccgggccag gcgcaggacc agcagcagcg tgccgatcgc gcacacggtg
     3661 cccagcagca gggcggcggc gcggacgctg cccaccaacc agccggcggc agccagcgcc
     3721 agccgccaga gcgtgtcgcg gatggccggg catggcgcgt tcggctgcag gccgtacgag
     3781 ccgtcgagga ccagggtgcg ggccacggcc atgtccgcgt aggcgcggtc ctccacgaag
     3841 accgagttgc ccgccgcccg gcgctcgacg agcagcgccg ccagcacgcc gatcagcatc
     3901 acgagcggca gcagccaccg cagcaggcgg tgcagcgccc atcgatgcaa ctggttgatt
     3961 cccgtcatag cggattcccg ctccccccgg aaccgcggcg cgcgatcagg gcccggcctt
     4021 cgggatgatc tggtcgggca cgaccaccgg gtactgcacc ccgcgcaccc agtactcgcg
     4081 gccgcggatc agcagccgcc gccccacgaa cgtttcaagc tgcgcgttgt tcccgtagac
     4141 gtagcacagc gtgcccaact ggttgccgcc gggattcacc aggcggaagc gcgagggccg
     4201 gcccagcaaa ttgcccgcgt gcttcagcac gccgtcctgc tgcaccgacc gcccctgccc
     4261 ctcgaacggg atcagcacca ggcccgcggg cggcggtttc accaccggct cgcgccccgt
     4321 ctgcacgcgc acgacccgct ggctcgccat ggtccccgcc gccggcgccg gcgggggcgg
     4381 agccgagggg ctcacgggcg gcgggggcgg gatggaactg gcggccacgg tcggcgcgga
     4441 atccgcgggc gcatcggcgc cggccggcgc ggccgcggac tccgccgcgg gcggccgcga
     4501 cgcgcccacc ggctcgacat agctgcggct gacccacagc gaggcgccgg gagggggggc
     4561 gatcttgagc cagtccgtga attcgccgcg gaccttgacc tcggccccgc gcggaagggt
     4621 ggcgacgacc cggtagttga tcccgggccc gccgcgcacc ttgagttccg tggcgacgac
     4681 cttgccgccc tgcacgaaat ccttgtggac ccagagatcc accgagtccg gcggcgccac
     4741 ctcgacccag gcatcgccga tcgaacgcgc ctcgaggatc tcgccgtcgg ccacctgccc
     4801 tacgacctcg gccttgagat cggcctgggc gcggaggttg acccgctgcc cgaccacgcg
     4861 gacgctctcg gcgcacgcgc atcccgccgc cagccagccc gccattccga gtgccacaac
     4921 gcgtttcatc tcgattctcc gtgagccccc gccgaccgat ccatccgtgc gcactctagc
     4981 ggagatcgcg gcgcgaatca atgaccccgc aaaccgggct tgtcaggggg gaggaccggg
     5041 ataagatgtc cgcgtgaacc atccgggcgc caacgaatcg gcggcgggac gatgggccag
     5101 cggctgggaa ctgggcccgc cggccgccgc gcggggggag ccttcgtcgg cgcgcctgcg
     5161 cacgatgaac cggcccggct ccgtcggccc ggacgcccag ccgccgggtt ggagcttcca
     5221 tttggagtgg cccgaggagc ggggccccgt cacgcccgag ctctttgcct gcggctatcg
     5281 gttgcgcttc gcgcgggcgc cggagtccgc gtgcgcggta ttcctggccg agcctggcct
     5341 gctgtgcggg catctcggcg cccgcgtcgc gccggcgctg ttctgcgcgg agacggatat
     5401 ccagacggcg ggcccgatga cctggatcga gagggagcac cagcacgtgg tgctggtgca
     5461 ggaagcggcg ggcgacggcc tccggttttg cctggcggtg gaaacccggg ccggccacgc
     5521 ggaggcggcg gagcgggcgc ggcgcggcct cgagcggaaa ttcgaggacc agttccaggc
     5581 cgagctcgcc cagcgcccga cgcggggagc gccgaccgaa ccgcgggcgc tcgatccggc
     5641 ggtcgcgcat ctcgccatcg aaacgctctt cgcgcggctc cgcccgccgc agggcctgct
     5701 gccgttccgc tgggtggagc aggatccctc cggccccgcg cgattttccc tgcgccacct
     5761 gtacccgctg gttttcgcgt ggacgcccat cgatcccgcc gtcgcgctgg atttagtgaa
     5821 atgcgcgctg tcctgtcagc aaccggacgg ccggatcccg tcggaagtct cgccggatta
     5881 cgccggagag cagtcgcccg ccgcgctgcc cctgatcgcc caggcggcgc ggcaggcctg
     5941 gagcgcgcac cgcgacccgg gcttcccgga cttcgccgcg ccccgcctgc gccgttatct
     6001 ccgctgggcc tgccggcgct acgacccgga acagaccggc ctgccgtgct ggccatccgc
     6061 cgacgaggcg ctgatcccgg aggcgcacga cgccgggctg gcctcccccg acctggcggc
     6121 ttttctgctg tgcgaaatcg acgcgtacct gcagctcgcg cgcgcctgcg tgaccaccgc
     6181 gggcgacgag gccacgtggc aggcggagat ccagcgtctc aaggacgccc tgcaccagac
     6241 cctttgggac gagcggcgaa gcgtcttcac cagccgatat gtccggggcg cggtgatcga
     6301 gcggctgacc ctttcctccc tgctgccgct tccggcgccc gggctgcggg ccgaccagcg
     6361 acaggccatt cttcaaggct tgcgggattc ggccggtttc cgcggccccg ctggcgcgcc
     6421 cgcctgggag cgttgggagg gcgacgccga accgccgccc acgcacgccc tgcaccagat
     6481 cctgctgctc gaagccctgc ggcgcgggaa cgcgccggac gaagcgcggg cgttatcaca
     6541 caccctctgc cggaccctgg ccggtctcga tttcgacggc ggcagcctgc cggaccggct
     6601 cgacgcgccc ccgcagaagc cggggatcat cgggacggcc gccgcggcgc tggccgtgat
     6661 cctggactcg cccgtggcgg tcgagcccgc ggccccggcc tccccggcgc tggcctggat
     6721 ggagcggcac cgggcctccc tcctcgccgg cgtggtcggc ctgctggtcc tggggctggc
     6781 gaccgtcgcg gtggccttcc tgtccaagac cgcgcccccg caggccaccc tgtccgcctg
     6841 gaccgggctg gccacccgct accaccagga agggcgctac gacgaggcgc aggaactcta
     6901 cgaaaagatt ctggccagcg gccggaacct gccgctggtg gaactcctgc tggccaacac
     6961 gctgctccgg aaaggcgaac tggaggaggc ggaggagcgc taccggaaac tggtggcggc
     7021 gcccgacccg cacccggtcg cctgtctgaa cctggccgtg acgctctacc tgcgcgggca
     7081 gaccaacgag gcgttcctga ccttccgcga attcgcggac aagtacggag aactgcagcc
     7141 ccagttggcg ttccgggcgc ggaccgccat gcaactgctc gcgccggacg aggatgccgc
     7201 ggcggttacc ggcctgccgg ccggcccggg ggaataaaaa gaaagaaggg cgttgcgcgc
     7261 caaggaggtc ggggtgagga accccatagg tgaacgcaca tccgcccttc aaaatcaata
     7321 tacttggacc gttgtctatt tcaagtgttc aaagaaaaaa tatccgcccg tatttgacgg
     7381 gtagggattt catgccctat tatcagggct tcgatcaacc ttgcaggata atattatgaa
     7441 gactcggcat ttttgtatag ggctttttct cgccgtcacc gcttttctcg ccgcggcatc
     7501 gggttgctac cggagggata tccgcaccct ggaggtgaaa gtgccccaga tgggctcgcc
     7561 cgaatgcgcc cgcatcgtgg aaaccgtgtt caacaagatg gacggcatcg agcaggtgga
     7621 catcgacctg gccaaccggc gcatcctggt gaagtacgac gggctcaaga tgggcatcgt
     7681 gaacatcgag tacctgctga cgaacgcggg tttcgacgcg aacgacaagc ccgcccggcc
     7741 ggaagcccgc gccaagctgc cgcccgaatg ccgctgaaaa catgaaaaca cgcctcgtct
     7801 ccgtccccct cgcccgccag cccgccgacg gctggctgct cttcgcgttc caggacgagc
     7861 ccgtgcccgt ccccggcagc gatccggtct cgcggcgcct ggaccgcatg ctgaaggact
     7921 ggcgcgcccg ccacgatttc atcggcaagc tgaaccaggt cgccgtgatt tcgtcctggg
     7981 agtcgctgcc cgcgcgctcg atcgtgctcg ccgggctggg aaagaaaaaa gattttcacg
     8041 tcgcccgctt ccggcaggcg gcggccgccg ccgcgcacac gctccgcaag caccgcctcg
     8101 cgaccctggc cgtctcgctc cgcgcgccgg acctgccgct cgcgccggaa ctcctggccg
     8161 gacacctcgc ggccgcgctg cactacggcc actacgcgtt cacgcgctac ctcacgaagg
     8221 acgaggacgg gccggatcgc cggatgccgg acatcctgtt tacggacgcg cccgtccgcg
     8281 cccccgcgtt ggcccgccgg ctcgcggata ccgtcgcggc gtctgaaatc ctgaaggagg
     8341 tgcgggacct ggccaacctc ccggccaacg aggcggcgcc agccgccctc gccggcgccg
     8401 cgcgcgccct ggcgcggaag cacgggctcg cgtgccgcgt catggacaag gccgcgctgc
     8461 agcgcaaggg ctgcggcgcc ctgctcgccg tcgggcgcgg cagccggcac gagccgcggc
     8521 tgatcatcct gcgctacccg ggcacggacc ggaagctccg tcctgtcgcc ctcgtcggca
     8581 agaccatcac gttcgacacc ggcggcatct ccatcaagcc cggcaagggc atggagtgga
     8641 tgaagtacga caagtgcggc ggcatggccg tgctcgccgc ggtcctcatg gccgcgcggc
     8701 tccgcctgcc gcgccccgtg atcgccctgc tgcccgcggc cgagaacatg cccggcggcg
     8761 acgcgacgcg gccgggcgac atcatccggt cctactcggg ccagacgatc gagatcctca
     8821 ataccgacgc cgaggggcgc ctgctgctgg ccgacgcgct gtcgctggcg gccgagtaca
     8881 agcccgccgc catcgtggac ctcgccacgc tgaccggcgc gtccgtcgtc gcgctgggcc
     8941 acgtgctctc cgccgtcatg ggcaaccgcc cggagatcgt ggagcaactg cgccaggccg
     9001 gggaaacttc gggcgaacgg ctgtggccgc tcccgctgct gccggaatac gacgaggaca
     9061 tcaagggcgc cttctccgac ctgaagaacg tcggcgaacg cggcgcgggc acgatcatcg
     9121 gcggcacctt cctccggcat tttgtccctg aatccatccc gtgggcgcac ttggacatcg
     9181 ccaacaccgc gtacgaggaa agcacgaagc cctacgcccc ggccgggccg accctgttcg
     9241 gcgcgcgcct gctcgtggaa tggctgcgga atctgtaaag gctcgctcat cgcaaccggt
     9301 agggcgcgct cgccgagcgc gccgcggcgg cctgggccag gccgccctac cagccaacca
     9361 gggacaaaag agaacatgac aaccttaacc caactcgatc tccccggtat ccccaaactc
     9421 cgcagcggca aggttcgcga agtcttcgac ctcggcgacc ggctgctgat ggtcgccacc
     9481 gaccgcatct ccgcgttcga ctgcatcctg cccaccggca tcccgcaaaa gggcgaagtg
     9541 ctcaaccggc tctccgcctg gtggttcgaa cggaccgccg ccatcgttcc gaaccacgtg
     9601 atcgccctcg acccctccgt ttttcccgaa cgactgcgcc cgttcgagca cctcctgcgg
     9661 ggccgggcca tgctggtccg gaaggcgaag cccctccccg tcgagtgcgt cgtccgcggc
     9721 tacctgatcg ggtccggctg gaaggattac acccgcacgg gctccgtctg cggcctgccc
     9781 ctgcgcgccg gctgccggct ggccgaccgg ctcgacgaac ctctcttcac gcccgcgcgc
     9841 aaggcggaca cgggccacga tgaaaatatt cctttcggcg aagtcgaaaa gatgatcggg
     9901 cccgaactcg ccgcccggct gcgcgcggta tcgctggccc tctatgagca cgcggccggg
     9961 cacgcgcgga cgcgcgggat catcatcgcg gacaccaagt tcgagttcgg gctggacggc
    10021 aacgaactga tcctgatcga cgagctcttc acgccggaca gctcccggtt ctggccggcg
    10081 gactcctacg cgccgggccg ctccccgccc tccttcgaca agcaattcgt gcgcgactac
    10141 ctcgagacgc tggactggga caagaccccg cccgccccgc ccctgtccga cgacgtggtc
    10201 cggaagacgc gcgagaaata cctcgaggcc taccgccgga tcacggagcg ggaggatatt
    10261 cggtaagcaa cgacccggcg accgtgcaac gaattactcg aaggcaaaat tccaccgcac
    10321 aaaatgccga tagctgaccg actgcgtatt ggcacgcagc gcccacgccg accatccata
    10381 caccggtgtt tctgaacatg ctgtggtcat attgtccgca tcggggtcgc caatccaata
    10441 ccacagatta gactggcagg cgaggctgaa tacatctatc agaggaacag cataggcgct
    10501 catttccccg acaggcatgg atccctccgc tgtctctatg ctgctgattg ataccgtccc
    10561 atctgcatgc agcacatggt ttgtatcctc gaagtatggc tggccgagaa aggcccatgt
    10621 attcgaatcg ccaccgcctg attgccgata tacacacaat cggtgcggcc ggagcaacag
    10681 gctggcatat gaagaggtcg ggcttcgcga atacgggatc aacatgccga tgcgagcgcc
    10741 ggattcgatg ccagtaagtg aataacccca ccagtgatac gtaacgtatc cgttggtata
    10801 tcccaagccg tacgcgcaat agtccataaa ctcaccgcca ccgtcaccaa tcgtagcgga
    10861 cgtgctgatc atgtagctct catactcatc gacggaattg gtgtcgacgc aaagattatt
    10921 gcacgcgtgc gcaggaggcc cgccgtctat cggaggatac tctcccggcg ccgacacctc
    10981 cccgatccga tcatgcctcc ttctctttcc gatcacgcct ccttcaccgg ccgtccattg
    11041 catgtcctga aagagccact tcgcgccctg atatccgtag atattggttt ccaaaatatt
    11101 gaacggtgta tttaccaaat aatccgcagg gagatcatat cgagccaact ggccggtgac
    11161 cgtgtagtag aagatattgc tgggataact gtacggaatc atcgccccat agcaggcgct
    11221 catggctaac tcattgatgt actgaccgat cagataactt gcccgctgct tgatttcaga
    11281 caaatgcacc ctcgggcagg tcgccatggg ttgaaaccac tcgccctccc cccaatccgt
    11341 ggccgccgcc atgcgttcat cagaagcccg ggcgcaccac gtgccgtctt cgatcaattg
    11401 cgccccatcc gccgccatcg gccgcgagga atagctctcc gcgctggcca tgacgcagca
    11461 aagaagaatg cagacaacaa cagccgtccg aatcactcct ctcatgggcc tctcgccatt
    11521 catcatttcc cgctctcgct cgcgttactt ggtcattcga ggattgctcc cgttccgcct
    11581 ccctcacacc agacttgcct cgatctactc atttgtctcg actctagata caaattcaag
    11641 gtataagtat atttccggaa aattagtagg atacgccacg gtgtgcagat actgtatttg
    11701 ctcttcactc cgagcttgat tcgtcgggat agatactggc gccgccatgt cagcaactga
    11761 tttaatgtga tccattacta tcccaggttg catcacctcg tgaggcgctc tatttgagac
    11821 ggccaatacg aactgttgag ctgccatctg tgcgttcgtg aaatccactc tggcccaaaa
    11881 atagtaccca taattcaggc atagaccgaa cagatcgttt gtacatgcag acaatcgatc
    11941 atcaacaatt tgatgtatct gagcatagtt cgtctgcacc catgcttcgt tgacggcact
    12001 caagaatggc tctttgtcac aagtctgagc atgaactgca gccgtccaga gaatgctgag
    12061 aacaacttcg attccagatg tcttcatggc gccaccgtca gcgcctccat tatttgtgtt
    12121 tggtgctagg ctggagttca atatacggaa atagagcggt atcgcgactg ggccactcct
    12181 tccgagccca ccattcgcgc catcttgcaa tatcatcctc agtaggagta ggtcctttcc
    12241 acaccacggg attcggaata attttggcca atgatcgcat ggccacgaca ttcagcggct
    12301 catagatcag cctgtcgagc ggcctctccc ctcggggctc ctccttcatt gtttcggaac
    12361 gctcagcatt gttcgtcaga agcatcagga gaagaccaac agcgtttgaa ttggcaacgt
    12421 actctagttt ctgtatcgct tggtcctgaa cccacggagt gcttaccgcc tctaattcgg
    12481 ctgcgatctc ggcaagcgcc tctgtgtccc caagctttac cagggccatt tgagcgcagg
    12541 cgccagcacc gccaaagctc ttgtttgaat ttaccgacat aatcgccttg agaaacggta
    12601 tcacatccgg attgcccctg tcccccagtt cgagaagggc ctcgcgactc cctgctcgcg
    12661 cgcttgcgat cagcgcctca acgctctcgt tcgcgttatt tgttatttga gggctgaccc
    12721 cgatcgcccc tttctgttta tcgtgatctg aagcgtggat atcttcacca gctagagacg
    12781 gtatttgtcg cttgaatgca ctggactttc ccgagcggag ccgctcgtct cgggcacgcg
    12841 ctttgccact cgtgatgatg tcgatcatgt gcagaacgtc ggtattgttc gcgaacgcaa
    12901 cacgaacctc gccaaggaac ttcttcacgc gcggatcgtc tgcgttgtac gagaggaacc
    12961 aacgattctc gtcacggggg ggaaagagga agatgttgag cagttcttct tgcgggagta
    13021 tgccttgcat gtgaagcgcc aagactcgat ccaggcaatt cgcgtactcc ttgggagaga
    13081 gataaagata cgagtgcaaa agaacgaggc gtgatgcacg atcaagtgag ctatccacga
    13141 gggcggaaaa tgtctgctgc catgtcgagt cgctggccgc gcaaagagcc cggaagtccg
    13201 gattggtttc aagaagccaa aggatgggtg acggatcggg aaacgcatta atagcctttt
    13261 cgcccagacc ctgaacgacc gcagcgataa gcgcacggct atctggtcgg gactcatcgc
    13321 cgaaggacgg caccagaccg gccaacatca cagcaaggca gattattccg attttcactt
    13381 cttcactcct cagttcgagt acgccctttg accggcaggc ttggtggcgc cggactgata
    13441 gtgtcgcctg taactactga actccgcctt ccggttcacg tttccaccaa cagcactact
    13501 agcgctgatc cattgcccat cgtagtcaac gatacccgca tgcccggacg agccgctgat
    13561 ttcgtatcca cttgcgcaga catggccggg ttgtggcgaa gcattgtcga aaagcagcgt
    13621 ccaattcgga atcggatatg gagcgttggg atcattgatg tccggcatcc ccgcccagtt
    13681 gttggccgag ggaggccccc caagatagac gccagccgta ggaggatcaa cgtccgcgcc
    13741 ggacccttca catacatcgg caacgaacac gttgcacttg ttctgaccgt cgaagagacc
    13801 gaatggatct cgccaagcgg gcaaggaacc ggcggatgaa tagtacgtgc tacctatgta
    13861 ccctaaagct tcttccctga cggcaagctg gatcgcgtta tcaacgaccc caaacgcgtc
    13921 gggctcgcct tccttgagtg tgctgtgcgg atcgtctgca gtccgttcgt atatcttctc
    13981 gcaagcgaca ccaccaacag tcagcaccgt ctcaacgcga aagatgccgc tttcggtcgc
    14041 cgtgtgaacg tactgggttc cgtccgcgtt ggcaccgaag ctctgccacg attcccacga
    14101 gccatcggca tccagttttt ggtagtacca cttgggctcg tcagcaggcg gcacaaagtc
    14161 tgacggcaag atgtgggcaa caaaggtcac gtcgtctccg ttctcgacaa cgagaccatc
    14221 ggcaggcgca ccgacgatct caacggactc caccataaga acggaacaaa tcagtttatc
    14281 cacacagaca gccccttttg gcccagggcc gtccgggtct acttcgaaaa caagcggctg
    14341 attgaggacc ggtacgaccg gttcgatgta gaagtccacg agttgcggct ttgtgataaa
    14401 tccgagggaa gcggctggat atgtgccagg cgggatatag tctccacccg aaaggatact
    14461 cgcgctattt cgcgcctgcg cggcattggt tcgccacaag cggaagtatc cgctcgccgg
    14521 aacatagccg ttggtcccga gcgttacacc ggacgggtcg gacgcgttgt acgagatgct
    14581 caccatggcc tgcgcgggtt cggtatatcc cgacagtaac acaggccacg gcacaaagaa
    14641 atcattggtt gaaaggtcat cagacgaatt gccctgcccg tcccgatcat agccatcggc
    14701 gaaatcgggg atgccgtccc catccgaatc gtcatcgttg acggccaagt aattcgagga
    14761 aaccagcttg taataggccg ccaagtcagc gcgcgccacg tactcggtcg ccatcccgga
    14821 tcccggataa ttcgtattga agacaggccc cacgtccgtg acaaccgtct tcgggcagaa
    14881 atccccgagc gtaccgacca gcacgctggc gatctcgctg gtcgctccgg ccggcgacac
    14941 atatatcgag gaggcggagg acgtgaacgc gatcgtgttc tgaagacacc agatatagcc
    15001 gccccagttg ctataggctt catgcgtgag cttgagccat ccgacgttgt cgtaaatctt
    15061 gccgggctgg ttgctgggac aggacgacca ggcattc
//
LOCUS       DAOOTK010000081        15048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2690_length_15048_cov_5.730608, whole genome shotgun sequence.
ACCESSION   DAOOTK010000081 DAOOTK010000000
VERSION     DAOOTK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15048
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_15048_cov_5.730608"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK634_09570"
     CDS             complement(<1..343)
                     /locus_tag="PK634_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98025.1"
                     /translation="MTWKPFHHPDLSLKVIRRQASAALLDFLAETGVVLMTRGRSLLW
                     GQGFKIPTSYRMAMCRLRKQGLIAYRRAGGQPPVLQVTAKGEEAREAAARPERLWQRK
                     WKGAWHLLSYDI"
     gene            500..1663
                     /gene="eboE"
                     /locus_tag="PK634_09575"
     CDS             500..1663
                     /gene="eboE"
                     /locus_tag="PK634_09575"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HOW98026.1"
                     /translation="MKLQEQPPVHLTYGLSIHPDETLAQLENSIRHKARAVRDRVAPG
                     RPFGLGLRISDAVSRELAEGDALPRLKALLDEHGFYVFTINGFPFGRFRGGPVKERVY
                     APDWRTDERVVYTMRLADQLAQLLPEGMTGSISTVPGSFRSWVRTDADLELIADRLSA
                     AAAHLAGIRDRTGREIHLGLEPEPGCLWETPAELVLFMREHLWTADRAGERARRHVGA
                     CLDTCHMALCFEEPEDVLAVYRNAGVRVSKIQLSAALESDGADVQALGRFAEPVYLHQ
                     AAARWPDGRRRSWPDLPGALRDLAGGPPPRTVRVHFHVPLPFGGGAGLRSTAALLRPS
                     FFRALRSGAAEHLEIETYSFDALPDGSAGDVVDSVAGEYRWTLARWADAGPPT"
     gene            1703..2851
                     /locus_tag="PK634_09580"
     CDS             1703..2851
                     /locus_tag="PK634_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOW98027.1"
                     /translation="MKTVCAASVLAGREAFEGLGEVVVRPDDRIGPADVRDADALIVR
                     SKTRVDARLLSGSSAQFVGTATAGFDHFDTAWLEAAGIAWCAAPGCNANSVSEYVVAA
                     LLHLACRDGFELAGRTLGVIGVGQVGSRVAAKAEGLGMRVLRNDPPLQLATGDPAFLP
                     LDEVLRGSDIVTLHVPLTRTGPCPTFHMAGCDFFARLRPGALFLNAARGEAMDTEALG
                     LALDRGIVSRAVLDVWENEPAISAALLDRVALGTPHIAGYSYDGRLNGTAAVYSEACR
                     FFEATPGWPPAECAGPAGGLLEADARGRSDEEVLRSLVRPVYDIEADDAALRRGPPGE
                     MVADRFRRLRHSYPDRREFPSWTVRLARGSPGLSGKARALGFRIPPCP"
     gene            2842..5862
                     /locus_tag="PK634_09585"
     CDS             2842..5862
                     /locus_tag="PK634_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98028.1"
                     /translation="MPLNRFTVLGPVLAAFLAGAGCAREDRDKPAAAGWMESVVVLDP
                     VFPSRGLDFLPAAERYPAAGDPAEILPRLGPGRVLIIPEARALPMHWWGPIADHIQRG
                     GPVLFCGPQPFASRVRLENGRAVTEEAVASNLVAGARAAGLSSSVRTWRHANDSGEIR
                     GAVRLAESGEAPWPAVEVEAEGFREWDLLSGHVPTGVVAAEENALAFYARGDVGTSRL
                     FVEGQERDGSQWGLPVEVATNWAPRLLHQSFFRYLGGGRGRGGPGDGLQLRQVSRIRI
                     GLDMARAPQSPGAHLYGLSEVRWAADPRPRAEISGWPELALVSPPCRRPPVAISALRD
                     LERGDKVRLAAHAAAIPPGLPRGTVETASWAGRWIPLYGAEDEWGRRQGWPVSLFLAG
                     RAGGKWRRWGWVALDPAGAPRPLTERIVSDAVRRLQGDLYFLYAGCPQWVYASREEIR
                     VRACWAGRPGISGVGVAAELRRDSDGQVLRRAVLPAVPPDRPFEISLGLAAAAGSLPE
                     DHRVVIVLEDLRRPGFFYDRQEQGIKVLPEKNAAPPPLGIVGGRFQWRGSPFGMAGVR
                     YEPPAPAGALSWLDPDCFDPDAVRQDLRRLREAGHNTLFIECRDERQAEPLRYVAEEA
                     RRQEMVIVLALPWMDPLDPEWDRAERLLKAVRAAENPTFAALDIEWNRELLGEARRQS
                     LGPAWRAWQAEAAGRTALPGEESYRLFLGDFASRRAGWIRRRLQLIGGAMMLTARMDA
                     DALSSWFDSEPPGSAHLDFLSVSLPGAGRLEPDWEAAAALAERARSAIAGRPLVWHGG
                     PVDVGPDPRDADLSNQERWFDLFVVLVARSQAAGLIAAAYPAAGCSGFGEDGGLVGAD
                     GKWRPAGQVLQIAVPRFRRDQIVPVREARMEAGARFNAEWGRIQAEGREVARAPGSPV
                     RLQPGQTLKAEVLNTGSASWAEGAASRVFVAVVRGEQMDSLPLRPLRRTEREWITWRA
                     PESGDWVLQCREPDGGRFGEPLVLEVRTNADGER"
     gene            complement(5883..6632)
                     /locus_tag="PK634_09590"
     CDS             complement(5883..6632)
                     /locus_tag="PK634_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98029.1"
                     /translation="MSASGRLVILSGPSCAGKSPLDKALARLYPALHARLRKLVLHNS
                     RAPRPGERDGVDYHFRPRQQVEALRSNPRYAVLDVRGDLQALDLEALAKQLEGGDAFF
                     EGNPFVGRVLLTHPSLAGVRRLSVFLAPLSREEILELKNPARRVALPDFVTDIMRRKL
                     LRRTRKQKGELALPDLENIEKRAGSAYQELKEAHAFEHVIPNHDGEDSENWDAFYWPL
                     GDARRALQAFAALLEGRPAAGVEKWERDLAP"
     gene            6698..7516
                     /locus_tag="PK634_09595"
     CDS             6698..7516
                     /locus_tag="PK634_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOW98030.1"
                     /translation="MSFGWRQRLAPALAALALLPAGVDAAKWRVMEDCTLIDDPSNDG
                     DSFHVRWHKRHYIFRLYFVDTPESEDSLPERVAEQAAYFGIDSASSIQLGKEAKKFTA
                     KFLEEGFSIQTMREDARGRSDRERFYGIVKNKAGEDLAESLVANGLARIFGVDKDVPE
                     EVRSSTFNRRLKGLELQAKNQKLGGWAPNLTRVEQRLAGLAAPVDVVGQTITTPQTVA
                     VFSLEDASRQLGLLQRGAQVKIVEALTPGMLKIQITTGSGAVIEGQCRRADLGI"
     gene            7673..10078
                     /gene="pheT"
                     /locus_tag="PK634_09600"
     CDS             7673..10078
                     /gene="pheT"
                     /locus_tag="PK634_09600"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOW98031.1"
                     /translation="MRLPISWLKEYVEFEDTPRGLADKLTFSGTEVEGLEIFGSTYEG
                     VVVGEILRVDRHPNADKLTLCAVNTGRGELTVVCGAPNARAGMKCPFAPAGVTLPNGL
                     KLKAARIRGVESHGMLCAEDELGLSDNHAGLMELDAAWAPGTPLAEVLGPPEAVLDLS
                     VTPNRPDCLSVLGMAREVAALYGSRAKWPEVRFAESDPAVEQLARVEVEDAEGCPRYT
                     ARVMAGVKIGPSPAWMKRRLELSGIRAISNVVDITNYVMLECGQPLHAFDQELLEEGR
                     IVVRRVRAGEKLATLDGIERAVTPDMLAICDARRPVAMAGIMGGAGSEIRDATHTVLL
                     ESACFRPQDIRRTSKKLGLSTESSYRFERGVDIGRTEWASRRAAQLMADLAGATVARG
                     VVDVFAKQPEPRRILCRFDKVNALLGLNESGERIKQVFESLELPVEKADAQGCTVRVP
                     SFRPDLEVEVDLAEEFARVHGLDNIPSPSPHAQIIEGSSDAAWRAVSSCRAHLVGLGL
                     REIMNYSFVSARFLDLFDPGDATRRLVIPNPVSQDHAVMRTALAPQMVDTLGRNLARQ
                     VEEAAFFEIGRVFGAGPDGQPFEEDRLAIGLMGPAGRTGLDRRRPVEPQEMYLWLKGA
                     WEELARAQGVEWRLEADAGCPYLEPGSAAALFIGGEPVGRMGLVAAKQRAEWRMTQPV
                     AVLEVRLAPLLGRAHVTPACRPVPAYPSVRRDVALIVDAAIRHEDVVRLIRKAAPAEL
                     ENIEIFDIFSGGSIGAGRRSLAYALTYRSASRTLTDEEANGYHEAIKQALKRELNAEI
                     RES"
     gene            10375..10562
                     /gene="ssrS"
                     /locus_tag="PK634_09605"
     ncRNA           10375..10562
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK634_09605"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10584..11927
                     /locus_tag="PK634_09610"
     CDS             10584..11927
                     /locus_tag="PK634_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOW98032.1"
                     /translation="MDELFEQEEKPEHAKKRPLAARVRPRTLDDVVGQEHLLGPGKLL
                     RRAIEADRLGSLILYGPPGCGKTTLAEVIARVTRRKFVRTSGVLENVAGLRAHLEAAR
                     NRRATSGVETILFIDEIHRFNKAQQDVLLPYVEDGAVILVGATTHNPFFFINSPLTSR
                     SQIFQLEALAPEHVRRLLDRALETDEALRALPARAEPEALDHLAKVCEGDARRALNAL
                     EIAALTSPKGPDGTVLVTRDAAADSIQKKAVVYDHDEDGHYDTISAFIKSVRGSDPNA
                     ALYWLAKMLYAGEDPRFVARRLVILASEDIGNADPRGLTVATSALEAVDFVGMPEARI
                     VLAQATTYLATAPKSNASYMGIEAAMDDVKNDRVLPVPRPLRSTGSKKAAKEFGHTGY
                     QYAHSFEGHFVDQEYAPTSKIYYEPTEQGYEETIKRRIEHWNEQRKKARRGKKPE"
     gene            11924..12535
                     /locus_tag="PK634_09615"
     CDS             11924..12535
                     /locus_tag="PK634_09615"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOW98033.1"
                     /translation="MKDALRKKMRGLRARWTAARVRAWSGKVRRRLAAWPVFRAARVI
                     AAYRATPGEPELGPLLRAAARRGVRICVPAFDGAKGAYAWAWWTPGAAEKAGQYGITE
                     PARRRAADPRNIELVLVPGLAFDAKGRRLGRGGGHYDRLLARTAGLRVGVAFEAQLVR
                     RVPRAAHDVNMDAVATDERMYVMKESLKRNPETAGRLLQAGRS"
     gene            12538..14106
                     /gene="rny"
                     /locus_tag="PK634_09620"
     CDS             12538..14106
                     /gene="rny"
                     /locus_tag="PK634_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOW98034.1"
                     /translation="MEELTTNWWMPTVFAFLLMVLGFYTRALFARAHAHTIEKRAELT
                     LEQAKKDAEVIAREAQIQARDEVMRARDAFEAEIRSRRQELMALEERIAAREGNLDRK
                     LGVLDKKEQVLDERMAEIEKAKEAVRTREAQIQAVVDQEKVKLQEVAGLSHEEARRIL
                     LQKVDEELRAETGAMIRKYQEEAKAGAEKEAREIITTAIQRYASEQVAEVTTSTLNLP
                     NDEMKGRIIGREGRNIRALEAATGVSFLIDDTPEVVVISSFDPLRREVAKVSLERLMA
                     DGRIHPARIEEVVAKVREEMDEVTRKAGEEAIFELGLQGVAPELVRTLGRLKFRHSFA
                     QNVLRHSIETAQLMGMMAADLGLDPVVAKRVGLFHDIGKAIDHQVEGNHAIIGADLLR
                     KCGEAAVVVNAVAAHHGEVEAGGNTYAVLATAADAISASRPGARSETTAIYLKRLEKL
                     EDIASSFRGVEKSYAMQAGREIRVIVEPGKIDDNEAMQMARNISKQIEQEMKYPGQIK
                     VTVIREMRCVEYAK"
     gene            14116..14901
                     /locus_tag="PK634_09625"
     CDS             14116..14901
                     /locus_tag="PK634_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOW98035.1"
                     /translation="MRILIVGDMVGSPGRAAFARVVGQMRQKGEVDFVVANAENAAGG
                     RGITGPLAREILDAGADVITLGDHAWDQKDLVTFIEKEPRVLRPANSAPGCPGRGWIS
                     VDTPGGRVTVLSVVGRVFMAPHFDCPFRTVDQVLKPDAGLGKVIIVDIHAEATSEKIA
                     LGRHLDGRVSAVVGTHTHVQTSDETILPKGTAYLTDLGMTGPHDGVLGRELAPVLKRF
                     ITGLPSPFDVATGDVRLEGLLVTVDAETGRAQKVKRVRERVEK"
BASE COUNT         2280 a         5067 c         5486 g         2215 t
ORIGIN      
        1 ggatgtcgta cgaaagcagg tgccaggcgc ctttccactt ccgctgccag aggcgctcgg
       61 gccgggccgc ggcctcgcgg gcttcctcgc ccttggccgt gacctgcagc acgggcggtt
      121 ggccgccggc ccggcgatag gcgatcagtc cctgcttgcg gagtcggcac atggccatgc
      181 ggtaggaggt cgggatcttg aagccctgcc cccagagcag cgagcggccg cgggtcatca
      241 ggaccacgcc ggtttcggcg aggaagtcca gcagcgccgc gctcgcctgg cggcggatga
      301 ctttcaggct caaatcggga tggtgaaacg gtttccaggt catggcgatc ctcctgcgct
      361 catggctgaa gacggtaaca aagtccttca cacccgtgaa gcattttgtt tcggcgaatc
      421 gcgcgggggg gcggagcggc ggaggagggc gtcctttgcg cttgtggacg gcgcgcgggc
      481 gcggtacatc ggcgacggca tgaaactgca ggaacagccg ccggttcacc tgacgtacgg
      541 cttgagcatc catccggacg agacgctcgc ccaactggag aactccatcc ggcacaaggc
      601 ccgcgccgtc cgcgaccgcg tggcgccggg ccgcccgttc gggctcggct tgcggatcag
      661 cgatgccgtc tcgcgcgaac tcgcggaagg cgacgcgctc ccgcgcctga aagccctgct
      721 cgatgagcac ggcttctacg tcttcacgat caacggattc cccttcggcc gtttccgcgg
      781 cggcccggtc aaggagcgcg tctacgcccc cgactggcga acggacgagc gcgtcgtcta
      841 cacgatgcgc ctcgcggatc aactggcgca gctgctgccc gagggcatga ccggcagcat
      901 cagcaccgtc cccggctcgt tccggagctg ggtccggacg gatgccgatc tcgaactcat
      961 cgcggaccgg ctctcggccg ccgccgcgca cctcgccggg atccgggaca ggaccggccg
     1021 ggaaatccat ctgggcctcg agccggagcc cggttgcctg tgggaaacgc cggccgagct
     1081 cgtcctgttc atgcgggagc atctctggac cgcagaccgc gccggcgagc gggcgcggcg
     1141 tcacgtcggg gcatgcctcg acacgtgcca catggcgctc tgcttcgagg agccggagga
     1201 cgtcctggcc gtctaccgaa acgcgggcgt ccgggtctcg aagatccagt tgagcgccgc
     1261 cctggaatcg gacggcgccg atgtgcaggc gctgggccga ttcgcggagc cggtctacct
     1321 gcaccaggcc gcggcccgct ggccggacgg ccggcgccgc tcgtggcccg acctcccggg
     1381 cgccctccgg gatcttgccg gcgggccgcc gccccggacc gtgcgcgtcc attttcacgt
     1441 gccccttcct ttcggcggcg gcgctgggct ccgctcgacg gcggccttgc tgcgcccgtc
     1501 gtttttcagg gccctgcggt ccggggcggc ggaacatctc gaaatcgaga cctacagctt
     1561 cgacgcgttg cccgatggct ccgccggcga cgtggtcgac agcgtggccg gcgagtaccg
     1621 ctggaccctg gcgcggtggg cggacgccgg cccgccgact tgaagcttgt ccgctccgcg
     1681 aatctccgac acaatcccgg ccgtgaaaac ggtgtgcgcg gccagcgtgc tggcgggccg
     1741 ggaggcgttc gaggggctgg gcgaagtcgt ggtgcggccg gacgaccgga tcggcccggc
     1801 cgacgtgcgc gacgcggacg cgctgatcgt ccgctccaag acccgcgtgg acgcccggct
     1861 tctttccggc tcgtccgccc aattcgtcgg caccgcgacc gcgggattcg atcatttcga
     1921 caccgcctgg ctggaggccg ccggcatcgc ctggtgcgcg gcgccgggct gcaacgccaa
     1981 cagcgtgtcg gaatatgtgg tcgccgcgct gctgcacctg gcgtgccgcg acggattcga
     2041 actcgcgggc cggacgctgg gcgtgatcgg cgtggggcag gtgggaagcc gcgtcgccgc
     2101 caaggcggag gggctcggga tgcgcgtcct gcgaaacgat ccgcccctcc agctggcgac
     2161 gggcgatccg gcctttctcc cgctggatga agtcctgcgc ggttcggaca tcgtcaccct
     2221 gcacgtcccg ctgacccgga cggggccgtg cccgacgttt cacatggccg gctgcgattt
     2281 cttcgcgcgg ctgcggcccg gcgccctgtt cctcaacgcc gcgcgcgggg aggcgatgga
     2341 cacggaagcg cttgggctgg cgctcgatcg cgggatcgtg tcgcgggcgg tcctcgatgt
     2401 gtgggagaac gagccggcga tctccgctgc gctgctggat cgcgtcgccc tcggcacgcc
     2461 gcatatcgcc ggctattcct acgatggccg cctgaacggc acggcggccg tctatagcga
     2521 ggcgtgccga ttcttcgagg cgacgcccgg ctggccaccg gcggaatgcg cgggtcccgc
     2581 gggaggcctc ctcgaggccg acgcccgggg ccggtcggac gaagaggtcc tgcgctcgct
     2641 ggtccggccc gtgtacgata tcgaggccga cgatgccgcg ctccgccgcg gaccgcccgg
     2701 cgaaatggtg gccgaccgct tccggcggct gcgccactcc tacccggacc ggcgcgagtt
     2761 tccctcgtgg acggtccgac tcgcccgcgg ctcgcccggg ctgtccggca aggcccgggc
     2821 gctgggattc cggataccgc catgcccttg aatcgtttca cggttctggg tccggtgctg
     2881 gccgccttcc tggcgggggc cggctgcgcg cgggaggatc gcgacaagcc ggccgcggcc
     2941 gggtggatgg aatcggtcgt ggtgctcgac ccggtgtttc cttcgcgggg ccttgatttc
     3001 ctgccggcgg cggagcgcta tccggcagcc ggggacccgg cggagatcct gccccgcctg
     3061 ggacccggcc gcgtgctgat tattccggag gcccgcgcgc tgccgatgca ttggtggggg
     3121 cccatagcgg atcacatcca gcgcggaggg ccggtcctgt tctgcggccc gcagccgttt
     3181 gcgtcgcgcg ttcggctgga gaacggccgc gccgtgacgg aagaagccgt cgcgtccaac
     3241 ctggtggccg gcgcgcgcgc ggccggcctg tcttccagcg ttcggacctg gcgccacgcg
     3301 aacgactccg gcgaaatacg cggcgccgtg cggctggccg aatcgggcga ggcgccctgg
     3361 ccggcggtcg aggtggaggc ggaaggattc cgggaatggg acctgttgag cggccatgtc
     3421 cccacgggcg tggtggccgc ggaggagaat gcgctggcct tctatgcccg cggcgacgtc
     3481 gggacctcgc ggctctttgt cgaaggccag gaacgggacg gctcgcaatg gggcctgccg
     3541 gtcgaggtcg ccacgaactg ggcgccccgg cttctccacc aatccttctt ccgctatctc
     3601 ggcggggggc gcggtcgggg cggacccggt gacgggctcc agctccggca ggtgtcgcgg
     3661 atccgcatcg gcctggacat ggcccgggct ccgcaatcgc cgggggccca cctgtacggc
     3721 ttgagcgagg tgcgctgggc cgccgatccc cgtccgcgcg ccgaaatcag cggctggccg
     3781 gaactcgccc tcgtgtcgcc gccatgccgc cggccgccgg tcgcgatctc cgcgctccgc
     3841 gacctcgagc gcggcgacaa ggtgcggctg gccgcgcacg cggcggccat tccgccggga
     3901 ttgccccggg ggaccgtcga gacggcgtcc tgggcgggcc gctggattcc cctgtacggc
     3961 gcggaggacg aatgggggcg gcggcagggc tggcccgtct cgctgttcct cgcgggccgg
     4021 gcgggcggca agtggcgccg ctggggctgg gtggcgctgg atccggcggg cgccccgcgc
     4081 ccgctgacgg agcgtatcgt gtcggacgcc gtgcggcggc tccagggcga cctctatttc
     4141 ctgtacgccg gctgccccca gtgggtgtac gcctcccgcg aggagatccg cgtgcgggct
     4201 tgctgggcgg gccgccccgg tatttccggc gtgggcgtcg ccgccgaact ccggcgcgac
     4261 agcgacgggc aggtgctgcg ccgggcggtg ctcccggccg tcccgcccga ccggccgttc
     4321 gagatcagcc tcgggctggc cgccgcggcc ggaagcctgc cggaggatca tcgtgtcgtg
     4381 atcgtgctcg aggatcttcg ccgccccggc ttcttctacg accggcagga gcaggggatc
     4441 aaggtgctgc cggagaagaa cgcagcccct cctcccttgg ggatcgtcgg gggtcgtttc
     4501 cagtggcgcg gctcgccgtt cggcatggcg ggcgttcggt acgaaccgcc cgcgccggcc
     4561 ggcgccctct cctggctgga cccggactgc ttcgacccgg atgcggtgcg ccaggacttg
     4621 cgccgcctcc gggaggccgg gcacaacacg ctgttcatcg agtgccgcga cgagcggcag
     4681 gccgaaccgc tgcgctacgt ggcggaagaa gcccgccgcc aggaaatggt gatcgtgctg
     4741 gccctgccgt ggatggatcc gctcgatccg gaatgggacc gcgcggagcg gctgttgaaa
     4801 gcggtcaggg cggcggagaa tcccaccttc gcggcactgg atatcgagtg gaaccgcgaa
     4861 ctcctgggag aagcccgtcg tcagtcgctc gggccggctt ggcgcgcctg gcaggccgag
     4921 gcggcgggcc ggacggcctt gcccggggag gaatcgtacc gccttttcct gggcgacttc
     4981 gcgagccggc gcgccggctg gatccgccgc cgcctccagc ttatcggcgg ggccatgatg
     5041 ctgaccgcgc ggatggacgc cgacgccctg tcctcctggt tcgattcgga gccgcccggt
     5101 tcggcccacc tcgatttcct atccgtgtcg ctgcccgggg ccggccgctt ggaaccggac
     5161 tgggaggccg ccgcagcgct cgcggagcgg gcccggtcgg ctatcgcagg ccggccgctg
     5221 gtctggcacg gcgggccggt cgacgtgggg ccggatccgc gcgacgcgga tctctcgaac
     5281 caggaacgct ggttcgatct cttcgtcgta ctggttgcgc gaagccaggc cgccggcctg
     5341 atcgccgccg cctatccggc tgccgggtgt tccgggttcg gcgaggatgg cgggttggtc
     5401 ggcgcggacg gcaaatggcg cccggccggc caggtcctgc agatcgccgt gccccgtttc
     5461 cgccgggacc agatcgtgcc ggtgcgcgag gcaaggatgg aggccggcgc ccgcttcaac
     5521 gcggagtggg gccgtatcca ggcggagggc cgcgaggtgg cccgggcgcc gggctcgccg
     5581 gtccgtcttc agccgggcca gaccctcaag gcggaggtgc tcaatacggg cagcgcttcc
     5641 tgggcggagg gcgccgcgtc ccgggttttc gttgcggtgg tccgcggcga gcaaatggat
     5701 tcgctgccct tgcggcccct gcgccggacg gagcgggagt ggatcacgtg gcgcgccccc
     5761 gaatccggag actgggtgct ccagtgccgg gagccggatg gcggccgttt cggggagccc
     5821 ctggtcttgg aagtgagaac gaatgcggac ggggagaggt agcccgcgcc cgatggggcg
     5881 gttcagggcg ccaggtcgcg ttcccatttt tcgacgccgg cggccggcct tccttccagc
     5941 agggccgcga acgcctgcag agcgcgccgc gcgtcgccca gcggccagta gaacgcgtcc
     6001 cagttctcgc tgtcctcgcc gtcgtggttc gggatgacat gctcgaaggc gtgggcttct
     6061 ttcagttcct ggtacgcgct gcccgcccgc ttctcgatat tctccaggtc cggcagcgcc
     6121 agttcgccct tctgcttgcg cgtccggcga agcagcttcc gccgcatgat gtccgtgacg
     6181 aaatccggca gggccacgcg ccgtgccgga ttcttgagtt cgaggatttc ctcccgcgac
     6241 agcggcgcga ggaacacgct caaccgccgc acccccgcca gcgacggatg cgtcagcagc
     6301 acccgcccga cgaacgggtt cccctcgaaa aacgcgtcgc ctccctccag ctgtttcgcc
     6361 agcgcctcga gatccagcgc ctgcaggtcg ccccgcacgt cgagcacggc gtagcgcggg
     6421 ttgcttcgca gggcctcgac ctgctgccgc ggccggaagt ggtagtccac gccgtcccgt
     6481 tcgccgggcc gcggcgcgcg gctgttgtgc agcaccagct tgcgcagccg ggcgtgcagc
     6541 gcgggataaa ggcgggccag ggccttgtcc agcgggctct tccccgcgca ggatgggccg
     6601 gaaagaatca ccaaacggcc ggaagcgctc atgctggttc ctccagtttt cagcctgacg
     6661 gatgacattg tgcccgacct gtggtaagaa tcaacccatg agcttcggat ggcgacagcg
     6721 gctggcgccg gccctcgcgg cgctggccct tctcccggcc ggcgtcgacg cggcgaagtg
     6781 gcgcgtgatg gaggactgca cgctgatcga cgacccctcg aacgacggcg acagcttcca
     6841 tgtccgctgg cacaagcggc actacatctt ccgcctctac ttcgtcgaca ccccggaatc
     6901 cgaggacagc ctccccgagc gcgtggccga gcaggccgcc tacttcggca tcgattccgc
     6961 ctcctcgatc cagctcggca aggaggccaa gaagttcacg gcgaaattcc tcgaggaggg
     7021 gttctcgatc cagaccatgc gcgaggacgc ccgcgggcgc agcgaccgcg agcggttcta
     7081 cggcatcgtg aaaaacaagg cgggcgagga cctcgccgag tccctcgtcg ccaacggcct
     7141 cgcccgcatc ttcggcgtgg acaaggacgt ccccgaggag gtccgctcct cgacattcaa
     7201 ccggcggctc aagggcctgg aactccaggc caagaaccag aagctcggcg gctgggcgcc
     7261 gaacctgacc cgcgtcgagc agcgtctggc cggcctcgcc gccccggtgg acgtcgtcgg
     7321 gcagaccatc accaccccgc agaccgtcgc cgtattctcc ctcgaggacg cctcccgcca
     7381 actgggcctg ctccagcgcg gcgcccaggt gaaaatcgtc gaggcgctga cccccggcat
     7441 gctcaagatc cagatcacca ccggcagcgg cgccgtcatc gagggccagt gccggcgggc
     7501 ggacctgggg atctaaccgc tgctttgcgg cgtcgcggag ctccgccctc caggcctttt
     7561 gaccggtgac gggcgagctc ccgcgagccc gaaaaaggct cgatactttt cccaatgttt
     7621 gggaaaatgc cccttagatt tttccagtca ttggaagaca ggcaggtacg ccatgcgatt
     7681 gccgatcagt tggctgaaag agtatgtcga gttcgaggac acgccgcggg ggctggcgga
     7741 caagctgacc ttttccggca ccgaggtcga aggcctcgag atcttcggct cgacgtacga
     7801 gggcgtcgtc gtcggcgaaa tcctgcgcgt ggaccggcac cccaacgcgg acaagcttac
     7861 gctctgcgcg gtgaacaccg gccggggcga gttgaccgtc gtctgcggcg ccccgaacgc
     7921 gcgcgccggg atgaagtgcc cgttcgcgcc cgcgggcgtg acgctgccga acggcctgaa
     7981 gctcaaggcc gcgaggatcc gcggcgtcga gtcgcacggc atgctgtgcg ccgaggacga
     8041 actgggcctg tccgataatc atgccgggct gatggaactc gacgcggcct gggcgccggg
     8101 cacgccgctc gccgaggtcc tcggcccgcc ggaggccgtg ctcgatctct ccgtcacgcc
     8161 gaaccggccg gactgcttga gcgtcctcgg catggcgcgc gaggtggcgg cgctgtacgg
     8221 gagccgcgcg aagtggcccg aggtccgttt cgcggaatcc gatccggcgg tggaacagct
     8281 tgcgcgcgtg gaggttgagg acgccgaggg ctgcccgcgc tacacggccc gcgtgatggc
     8341 cggcgtgaag atcggcccgt cgcccgcctg gatgaagcgg cgcctggaac tctccggcat
     8401 ccgcgcgatc agcaacgtcg tggacatcac gaactacgtc atgctcgagt gcggccagcc
     8461 gctccatgcc ttcgaccagg aactgctgga ggaggggcgg atcgtcgtcc ggcgcgtccg
     8521 cgcgggcgag aagcttgcga cgctggacgg catcgagcgc gcggtgacgc cggacatgct
     8581 ggcgatctgc gacgcgcggc ggccggtcgc catggcgggc atcatgggcg gcgcgggcag
     8641 cgagatccgc gacgcgacgc acacggtcct gctcgaaagc gcgtgcttcc gtccgcagga
     8701 tatccggcgc acgtcgaaga agctcggcct ctccacggaa tcgtcctacc gcttcgagcg
     8761 cggcgtggac atcgggcgca cggaatgggc gagccgccgc gcggcgcagc tgatggcgga
     8821 cctcgccggc gcgaccgtcg cgcgcggcgt ggtggacgtc ttcgcgaagc agcccgagcc
     8881 gcgccggatt ctctgccggt tcgacaaggt caacgcgctg ctcggcctga acgagagtgg
     8941 cgagcggatc aagcaggttt tcgagtcgct cgaactgccg gtcgagaagg cggacgcgca
     9001 gggctgcacg gtgcgggttc cgagcttccg gccggacctc gaggtcgagg tggacttggc
     9061 cgaggagttc gcgcgcgtcc atggcctgga caatatcccg tcgccgtcgc cgcacgcgca
     9121 aatcattgaa ggatcgagcg atgcggcttg gcgcgccgtc tcctcctgcc gcgcccacct
     9181 tgtggggctg gggctgcgcg agatcatgaa ttacagcttc gtatcggccc ggtttctgga
     9241 cctgttcgat cccggcgatg cgacccggcg gctggtgatc ccgaatcccg tgagccagga
     9301 ccacgccgtc atgcgcacgg cgctggcgcc gcagatggtg gatacgctgg gccgcaacct
     9361 ggcgcgccag gtcgaggagg ccgcgttctt cgagatcggg cgcgtgttcg gcgcggggcc
     9421 ggacgggcag ccgttcgagg aggaccggct cgcgatcggg ctgatgggcc cggcggggcg
     9481 gacggggctg gaccgccgcc ggccggtcga gccgcaggag atgtatctct ggctcaaggg
     9541 cgcgtgggag gaactggcgc gcgcgcaggg cgtcgagtgg cggctggagg cggacgcggg
     9601 ctgtccgtac ctggagcccg ggagcgcggc ggcgctgttc atcggcggcg aaccggtcgg
     9661 ccgcatgggc ctggtggccg cgaaacagcg ggccgagtgg cggatgaccc agccggtcgc
     9721 cgtgctcgag gtgcggctgg cgccgctgct gggccgcgcg cacgtgacgc ccgcgtgccg
     9781 gccggtcccg gcgtatccgt cggtccggcg cgacgtggcg ctgatcgtgg acgcggcgat
     9841 ccggcacgag gacgtggtgc ggctgatccg gaaggcggcg ccggcggaac tggaaaatat
     9901 cgaaatattt gatatatttt ccggtggaag catcggcgcg ggccgtcgaa gtctcgccta
     9961 cgcattgact taccggtcgg catcacggac cttgacggac gaggaagcca acggctatca
    10021 tgaagccata aagcaagcgc tgaagcgcga gctgaacgcg gagatccgcg agagctgaac
    10081 gatcaatttg aacaaggcag agtagattcc catgattccg tcatggaatg aactagtgtt
    10141 cgtattcgca ggcgcgggat ggcgggctgc tgtgaatcca ttggacagtt ggcagcccct
    10201 tccgaaatac gcggagcccg cgcggccccg gagggcttcg gcgcccgggg ttcggccgag
    10261 agcggggccg ggtccgcggg gaccggcgcc cgggccgacc gccggatagc cggcggcctg
    10321 ttctttgcca aagaaaaggg atttcctccg ggccgcgggg tccgggggta aaaaaatacc
    10381 ctgccttgtt cgtgatcggg ccgaattctc tgacccaaca ttaaccatca gggagctcga
    10441 agtccgggcg gcgcccacat gcccctgtct aaacccgggg gacttggaga ccgatcggcg
    10501 gagcacccac ctcgttaagc gaggttcagg gatagagccc caacggcaat ggcggggtat
    10561 ttttttgccc ccggagcgga gcgatggacg agttattcga gcaggaggaa aaaccggagc
    10621 acgcgaaaaa gcggccgctg gccgcgcgcg tgcgcccccg gaccctcgac gacgtggtcg
    10681 ggcaggagca cctgctcggc ccgggcaagc tgctccgccg cgccatcgag gccgaccggc
    10741 tcggtagcct gatcctttac ggcccgcccg gctgcggcaa gaccaccctg gccgaggtca
    10801 tcgcccgggt cacccgccgc aagttcgtcc ggaccagcgg cgtgctggaa aacgtcgccg
    10861 ggctgcgcgc ccacctcgag gcggcccgga accgccgcgc gaccagcggc gtggaaacca
    10921 tcctcttcat cgacgagatc caccgcttca acaaggcgca gcaggacgtc ctgctgccct
    10981 acgtcgagga cggcgccgtg atcctggtcg gcgccacgac gcacaatccc ttcttcttca
    11041 tcaactcgcc gctgacgtcc cgctcgcaga tcttccagct cgaggccctc gcgccggagc
    11101 acgtccggcg cctgctcgac cgcgcgttgg aaaccgacga ggccctgcgc gcgctgcccg
    11161 cgcgcgccga accggaggcg ctcgaccacc tggcgaaggt ctgcgagggc gacgcgcggc
    11221 gcgcgctcaa tgccctcgaa atcgccgcgc tcacgtcgcc aaaagggccg gacgggaccg
    11281 tcctcgtcac ccgcgacgcg gcggcggact cgatccagaa aaaggccgtc gtctacgacc
    11341 acgacgagga cggccactac gacacgatct ccgccttcat caaaagcgtg cgcggctcgg
    11401 acccgaacgc ggcgctctac tggctggcga agatgctcta cgcgggcgag gatccgcgct
    11461 tcgtcgcgcg gcggctggtg atcctggcct cggaggacat cggcaacgcc gatccgcgcg
    11521 ggctgaccgt ggcgacctcg gcgctcgagg ccgtggactt cgtcgggatg cccgaggcgc
    11581 ggatcgtcct ggcccaggcg acgacgtacc tggcgacggc gcccaagagc aacgcctcct
    11641 acatggggat cgaggcggcg atggacgacg tcaagaacga ccgcgtcctg ccggtcccgc
    11701 gcccgctgcg cagcacgggc tccaagaagg ccgcgaagga attcgggcac acgggctacc
    11761 agtacgccca cagcttcgag gggcatttcg tggaccagga atacgcgccg acatcgaaga
    11821 tctactacga gcccacggag cagggctacg aggagacgat caagcggcgg atcgagcact
    11881 ggaacgagca gcgcaagaag gcgcgccgcg ggaagaagcc ggaatgaagg acgccctgcg
    11941 aaagaaaatg cgcggtcttc gagcccgctg gacggcggcg cgggtgcgcg cgtggagcgg
    12001 gaaggtgcgg cggcggctgg ccgcgtggcc ggtcttccgc gcggcgcggg tgatcgccgc
    12061 ctaccgggcg acgccgggcg agccggaatt gggtccgctc ctgcgcgcgg ccgcgcggcg
    12121 gggcgtccgg atctgcgtgc cggcgttcga cggcgcgaag ggcgcctacg cgtgggcctg
    12181 gtggacgccg ggcgcggcgg agaaggccgg gcagtacggc atcaccgagc cggcccggcg
    12241 gcgggcggcc gatccgcgga acatcgaact ggtcctggtc ccaggcctgg ccttcgacgc
    12301 gaagggccgg cggctggggc gcgggggcgg gcactacgac cgcctgctgg cgcgcacggc
    12361 gggccttcgc gtcggcgtgg cgttcgaggc gcagctcgtg cgccgggtgc cgcgcgccgc
    12421 gcacgacgtg aacatggacg ccgtggcgac ggacgaacga atgtacgtga tgaaggaatc
    12481 cctgaaacga aaccccgaaa ccgccggtcg ccttcttcaa gccggcagga gctgaacatg
    12541 gaagaactga caaccaactg gtggatgccc accgtattcg ccttcctgct gatggtcctg
    12601 ggtttttaca cccgggcgct ctttgcccgc gcccacgcgc acacgatcga gaagcgggcc
    12661 gagctgacct tggagcaggc gaagaaagac gccgaggtca tcgcccgcga ggcgcagatc
    12721 caggcccgcg acgaggtcat gcgcgcccgc gacgccttcg aggcggagat ccggtcgcgg
    12781 cggcaggaac tgatggcgct ggaggagcgc attgccgccc gcgagggcaa cctggaccgc
    12841 aagctgggcg tcctggacaa gaaggaacag gtcctggacg agcggatggc cgagatcgag
    12901 aaggccaagg aggcggttcg cacccgcgag gcccagatcc aggccgtcgt ggaccaggag
    12961 aaggtcaagc tgcaggaggt cgccggcctg tcgcacgagg aggcccggcg gatcctgctg
    13021 cagaaggtcg acgaggagct gcgcgccgag acgggcgcca tgatccggaa ataccaggag
    13081 gaggccaagg ccggcgccga gaaggaggcc cgcgagatca tcacgaccgc catccagcgc
    13141 tacgcctccg agcaggtcgc cgaggtcacg accagcacgc tgaaccttcc gaacgacgag
    13201 atgaaggggc ggatcatcgg gcgcgagggc cgcaacatcc gggcgctcga ggcggcgacg
    13261 ggcgtgagct tcctgatcga cgacacgccg gaggtcgtcg tgatctcttc gttcgatccc
    13321 ctgcgccgcg aggtggccaa ggtctccctg gagcgcctga tggccgacgg gcgcatccac
    13381 ccggcgcgga tcgaggaggt cgtcgccaag gtccgcgagg agatggacga ggtcacgcgc
    13441 aaggccggcg aggaggcgat cttcgagctg ggcctgcagg gcgtggcgcc cgagctggtc
    13501 cggacgctcg gccggctgaa gttccggcac agcttcgcgc agaacgtgct gcggcattcg
    13561 atcgagaccg cgcagctcat ggggatgatg gccgcggacc tcgggctgga cccggtcgtc
    13621 gccaagcggg tcggcctgtt ccacgacatc ggcaaggcga tcgaccacca ggtcgagggc
    13681 aaccacgcga tcatcggggc ggacctgctc cggaaatgcg gcgaggcggc ggtcgtggtc
    13741 aacgccgtcg cggcgcacca cggcgaggtg gaggcgggcg gcaacaccta cgcggttctc
    13801 gcgacggcgg cggacgccat ctccgcctcg cgcccgggcg cgcgttcgga aaccacggcc
    13861 atctacctca agcgcctgga gaagctcgag gatatcgcga gcagcttccg cggcgtcgag
    13921 aaaagctacg cgatgcaggc gggccgcgag atccgcgtga tcgtcgagcc gggcaagatc
    13981 gacgacaacg aggccatgca gatggcccgc aacatcagca agcagatcga gcaggaaatg
    14041 aagtatcccg ggcagatcaa ggtcaccgtg atccgggaaa tgcggtgcgt ggaatacgcg
    14101 aaatagggag accccatgcg aatcctgatc gtcggcgaca tggtgggatc cccgggccgg
    14161 gcggcctttg cccgcgtggt cgggcagatg cggcagaagg gcgaggtgga cttcgtcgtg
    14221 gccaacgcgg agaacgccgc gggcggacgc gggatcaccg gcccgctggc tcgggagatc
    14281 ctcgacgcgg gagcggacgt catcacgctg ggcgaccatg cgtgggacca gaaggacctg
    14341 gtgacgttca tcgagaagga gccgcgcgtg ctgcgcccgg cgaattccgc gccgggctgc
    14401 cccggccggg gctggatcag cgtggacacg cccggcgggc gcgtgacggt gttgagcgtg
    14461 gtcgggcggg tcttcatggc gccccacttc gactgcccgt tccggacggt cgaccaggtc
    14521 ctcaagccgg acgcggggct gggcaaggtg atcatcgtgg acatccacgc cgaggccacc
    14581 tccgagaaaa tcgcgctggg ccggcacctc gacggccggg tcagcgcggt ggtcggcaca
    14641 cacacgcacg tgcagacctc ggacgagacc atcctgccga agggtacggc ctacctgacc
    14701 gacctcggca tgaccggccc gcacgacggc gtcctgggcc gcgagctggc cccggtcctc
    14761 aagcggttca tcaccggcct gccgtccccg ttcgacgtcg cgaccggcga cgtgcggctg
    14821 gaaggcctgc tggttaccgt ggacgcggag accggccggg cgcagaaggt caagcgggtg
    14881 cgggagcggg tggagaagtg aatagtgaat ggtgaattgt gaatagtggc cggaccctga
    14941 accctgaacc ctccagacct ccggacggcc gccggattta ccgggtctcc gagctcaccc
    15001 gcctcatccg ggtcgcgctg gagaacgagt tcggcagcgt ctgggtcg
//
LOCUS       DAOOTK010000082        15004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2707_length_15004_cov_4.608669, whole genome shotgun sequence.
ACCESSION   DAOOTK010000082 DAOOTK010000000
VERSION     DAOOTK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15004
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_15004_cov_4.608669"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5166
                     /locus_tag="PK634_09630"
     CDS             <1..5166
                     /locus_tag="PK634_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOW98036.1"
                     /translation="AANFTVNTNTYWSTDTDGGAFTLGASGGNLVLTFTPNTSEPTVQ
                     ATTVAFSVVGVTSMTVGWTPGNGANRVVVAHAGAAVDDDPDDAVPYAADPNFGDGAEI
                     GTGNFVVYNGSGSSVPVTGLSADTVYHFRVYEYNGSGGSENYNTNAASGNPASQTTLD
                     DAPTAAATALAITAVDSTTMSLGWTRGDGANVLLVGRSGAAAEEPTDGTTYAADPAWG
                     SAGAVGAASKALYLDSGTSEDLTGLSAETLYHFAAFELNGSGGSENYLTAGAPVTNLY
                     TLSTEPSAHAGSFAATAVSKSQIDLSWSAAAGADAYMILIRQGTDPAGTPTDGRAYVV
                     GDTFGSDTVTGLVAGTSFSAYNLSPNTQYNFSIIPYNWDASHAQTYNYRTAATIPTAN
                     DTTWARASRVIKESFNSYADFNNLAGANGGTGWEGAWSGAWDEGDIWIDDLNFSVSLQ
                     LLPAQEGDYTRKAKFDVDSNGKRSAIKRQFEAITSGQLYAMWIMQGGENNASRFWGVS
                     FGTNANYGFQAFGVGKTRNDGLDLGISIPNLYSNQTVQTTDWDLNAGNPYLIAIKYDF
                     DTDLLQVKAWYYTVTDIFSMQEPGPTGVADGWDMERSIGGIDKLDYLFLEAGSADAGG
                     NAGECFFDHIRIGTNWYETMVLGGEDDPSAPVPPTAALVYIGTNYSGWDKSLTKTNVY
                     DGELANTNDPIDFAVRWTDATGMFVTNDPSSVQNISGTKGRVSPNWDPLSIGAATNAF
                     GFDRVFTNFYGNSGDGSVTSYQYSAFSVTNLDIDAYTYYITLGGENEDQSSGEYTADP
                     PAGGQDTYGSRGILVNEPLRFYVFDDDTNAPALADIYSVSGIVATLRDLHITVGTSNT
                     YSSGDTTNRVFRTADGNLAALSDSNPLRLSLGATDPSGVSRGSMSTSWNMSVTIGSVI
                     QSNKTEFSADESSPLAAAGLTTNVWRFNSLTYNQIGDLFTAHTNKVEATVPDADMDRV
                     NDQMVLTNQQYGLFAVYDDDVLGPALGDITVVGAAASGSAILQTGFEASEGWPIQGSG
                     EPWDIPITNGLGTGVWHGTGYVNIGDPRSGVRKGGMTVAGVGQYFELPARDNPGTLSV
                     YARLSSAGASRELAVERWDGAEWTGCGTNTVTSDSYQRYDWDVDWIENGVTLRLVRVG
                     TDGSPGIFLDDLELISADPIWLSATAITSFWNAAVDASGVYEYRAVAPSYSASEPSQM
                     TDGTSLGAGTQNVFNVAGCQGVLTGFVFSVDDDNDRANDRTNGNNSAFLVRIDTNPPP
                     VIANVTAIEGEDPTSEAIVQWTAATNAGERAAPSYAVLSAWDTYRVYYTDDETEPTTG
                     SPYVDKTYDAALTNMSVTNVTVTNLIFDTTYKFRVAGRDQAGNLGPLSDTFTIVLPGF
                     NVTQGMVQVGSGGIRFPEIYWKAATNASGGVSREYDLLFADAISFSDSLSNQWALVES
                     GWTNALPDTGDVARAAPTLMVDTMRFFRAASADRWLIDRSPRVASEEVYGMKTVWLYP
                     GQNWIGLSVIPDSNTLQNVLGHELPAGSMYTDPDSTIVSWYNRASNEVVKKDVWLLDW
                     GASNQWRTGTGWGGLNQPADYRSLPTDEGFVVVIPTNTSETRLMIHLGRVPTNIQEQV
                     IEPHAYNLVNMRVPAKLKPSQMNLVESGFQGGPNSLISDRLRKLNRELKSTGRDVWYN
                     TTEQKWYYAGAGLADDFYITPDDGFLIWTRASASAWTWTNALPYSAPTRLMNP"
     gene            5288..6811
                     /locus_tag="PK634_09635"
     CDS             5288..6811
                     /locus_tag="PK634_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98037.1"
                     /translation="MNEEWKEERSGGSRRRWSPRLPFAGLLILCGTLAYRPALTRIFE
                     SDQLGYFAELQGQTTLASGLSLLDYGARRQYFKGDQVLYRPLLFAGLALENAAFRRDF
                     RLWNAANLGVHLAVSYLLFEILWRTRRSVLACGFALWFALLMSNFELVTWSHLGGYML
                     GYGLLLLALHARGQMEMDQAGARWFWVYGFSMIGAMLAHEIAVIAGVGMAAHGFWTFG
                     RRPDAGRRRWVASLLAPILVYAALYARHALRCDRFFWWSAPAGASVAAGLADLPSFLS
                     GWAQHILLPRQDQLICSVGSRSAWADWPGGLPPATLLAGALWAGLLAVLWPGLRSQRN
                     GSGWPFGRFVAFLVAAYAAMNLLGRPAYAMQVPYYDYFPALLGLAWLCSRVDVTLVGR
                     KRMIGACAGLLLLAAVNGWQVRQIGNRLHAMHRPWAQYLGWVEATVRPRLSIPGYSFD
                     VRGTPADMNLEGDLAIGFPDQNQVVIRNVLTILYGNFHNVAAPAEVLVFPGLDAGPR"
     gene            6811..8949
                     /locus_tag="PK634_09640"
     CDS             6811..8949
                     /locus_tag="PK634_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98038.1"
                     /translation="MKSDMFSRAAQEWISGRKGRCALAGLAAMAAAFVLYAPSIGHDF
                     SLVDDRYFVEDNPLVQGGLTWPGIRSAWTAVHAGYWAPLLWISFMLDMEISGGSPWSF
                     HLANVVLFSLNAGLVFLLIRRWTGRGGLALAAALLWAFHPARVESVAWITERKDVLSG
                     LFFLLGLWFYTASRQASSSPSCIFLSWFCMLLGGMAKQVVLVMPVALVLLDIWPLGRT
                     DWNRFWKDAGRLVAEKWAFWLLGLLLAGMALRAQAGDQALIPVSAGYRLAMIPVHYLF
                     YLVKLVWPAGLAPLQDDLLWSGWKMAAGLGALAGVTALLWKLRVKAPWALWGWMWFVA
                     LLLPLSGVVWSGSERVAVRFLYLPQIGLTLAVVLAMDALGRARGSSGGWLRVLLAVVL
                     LFSGIATLRTLSHWRDRDSFGLWIWECHPQQGGACAMGGDTLMAWGEWAEALKAFEQG
                     ASVGDKACFLRQGMVWNHLGQMERTAAAWVEFERKWGLPLLGFADWEPAPERELAWSV
                     RGQVLLARGDHAGAIAALKEAVRWEPDPAALVVADYLRACHEAGRPEEGAEAAERLAA
                     AGDVRVRAWRDLFPFYAEMWKRGARGYAYGYLAEYAKRFPGDAVNLNYMAWLLATAMP
                     DGLAHARMEEWPQAAVRWAELALASGDPSLPGVWDVLAAARANAGDSTGAVRAAEKAR
                     DLAKEKKDDALATDIEKRILKYRMGLAWRE"
     gene            9200..9691
                     /locus_tag="PK634_09645"
     CDS             9200..9691
                     /locus_tag="PK634_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOW98039.1"
                     /translation="MQNELDHENLDVYQVELRFVEWSTDLMVELVESAEARGRRIAEV
                     CDHLDRAGLSSLFNTAEGNGKRPMKTRAKFFDDARGSATECAACLDALVAKHACSEKR
                     VEQGKMLLVRIVAMLTKLVDRFSVTDRVMEAGMEYEVEDENEDEEEDENEGVKERGDE
                     QEA"
     gene            9703..11880
                     /locus_tag="PK634_09650"
     CDS             9703..11880
                     /locus_tag="PK634_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98040.1"
                     /translation="MRADKRKLAEPTRDQPRRLFSGRGRAAGACLLAMAAAFALYAPT
                     IGYDFVTLDDPIYVETSPVVGDGFSWKGLRLAWTTAPENYWAPLLWMSFMLDMEISGG
                     PPWSCHLTNVILFSLNVGLLFLLIRRWTGRVGLALATTLLWAFHPTRVESVAWITERK
                     DVLSGVFILLGFWFYTASRQSSSSSSSSSSSSSSYILLSWLCMLLGGMAKQVVIVMPV
                     AFLLLDIWPLGRTDWSRFWKDAGRLIAEKWAFWLLAIGFASLPIAFHVARKAVVSVPV
                     LHRVGMIPVHYLFYLAKLAWPTRLMPLQPDLPSWWELLAGAFCVLAGLTWGAWRLRHR
                     FPLALWGWLWFAGLLFPLSGVIWAGAERVATRFMYLPQIGLTLAAAWAAADWIRSRGW
                     SARRGAAACALVLAVWGGMSLALSRHWRSRDHFGIWVWTYNPGHPVACMLGGDGHLSQ
                     KNWAQAMRAYEAGSAFYDTHCFLRLCMIWNCCGWPNLTEDAWPRFEKGLGKTLMEAVR
                     DQRPLERIFIWRIRGQCMQARGDLDGAFGAFKEAVALEKDPTAFAVAEYLRVCHETGR
                     TNEAAEAAERMTKAAGKPRRTWGDLFPCYAQIWLDGARGYAYGYFLEYVKRHPRDAVA
                     FNNMAWLLATAKPVDVNHASMEEWPAKAVEWAARAVELDGGKAATAWDTLGAARANAG
                     DFPGAIQAAKRGAELAREGGGLDLAAQIEARLASYRAGKAWIE"
     gene            12354..>15004
                     /locus_tag="PK634_09655"
     CDS             12354..>15004
                     /locus_tag="PK634_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOW98041.1"
                     /translation="MKHETMSKPVARRGWRPLALAIAAAAWCLAPMRSEAAYSGSGTF
                     TLITSTADITSGGYYVFVGGGTANAMASSALATVSTAALSAQAVTITASAIVDPAVGR
                     VWRIDAVSSSYGIYNEAGSIYIGAPAANGMIRSASASSATIWHWTFATTASSRFLIQN
                     VGAASARKLQYNSSSPRFAAYSSASTYVELYKMNTCENLSAVTNLQQQSQYDTRWDSG
                     AGTFDTGGSTELGMWAGGDQGYTVAWKTFRTASATTSSARELQVGDEFSITVYSYGCY
                     YGEIGVSLNDGGSTGTPWANRVSGSRLSVRQDGGNYGSGGGIGSWYAIGSGAGESFTN
                     TPAGSSGADYTVKVKVTSSSTFNVDLNGKTKYDWTMAGSPATSARIDAYSIFLNDDRR
                     YQWDWGDNRQNSYWKQTATVRDTGAVEFGGGNGTSTINGLITDGLGAACASGTTANRL
                     YKIGTGTITLGCTTNTYTGGSAAENGILQGSADLCFGAAPASLVTNHFEVWSSGTLQF
                     TGSFALNANRGIRLGDVATPKLGVTSGNAVSYGGSMVGTANWQKVESGMLTLTGFNTN
                     SGVAHITAGILAFGADLAAGPVPGGAGEKINVWSTGTLGFTNVFTLHANRTIELGTTA
                     GPRIWVAPDSTATVNGVIQNSAAWTNIGKGTLILAGNNTFSGNLSITQGVVRVTNANG
                     LGGTGGNTYVANSTASALELAGGITTAAEPLFLNGTGIASGGALRNISGNNTFAGAIT
                     LQSTGVRINSDSGILTLNSATAISGSGYNLTFGGASNIVVSSAIGTGAGTVTKDGAGL
                     LTLTGYNTFSGALTISAGAVQLGANGTAGAVTAASIVNNSALAFYRTDDTNYLGVISG
                     TGYVTNRGAGMVLFTNNNSYSGGTVIDA"
BASE COUNT         2461 a         4875 c         5263 g         2405 t
ORIGIN      
        1 gccgcgaact tcacggtcaa caccaacacc tactggtcca ccgacacgga cggcggcgcg
       61 ttcaccctgg gcgccagcgg cggcaacctg gtcctgacct tcacgccgaa cacctccgag
      121 ccgacggtgc aggcgaccac ggtcgcgttc agcgtggtcg gcgtgacctc gatgaccgtc
      181 ggctggacgc cgggcaacgg cgccaaccgg gtggtcgtgg cgcacgcggg cgccgcggtg
      241 gacgacgatc cggacgacgc ggtcccatac gcggcggacc cgaacttcgg ggacggcgcc
      301 gagatcggga ccggcaactt cgtggtctac aacggcagcg gcagctccgt gccggtgacg
      361 ggcttgagcg cggacacggt ctaccatttc cgtgtctacg agtacaacgg ctcgggcggc
      421 agcgagaatt acaacaccaa cgcggcctcg ggcaacccgg cgagccagac cacgctggac
      481 gacgcgccga cggcggcggc gaccgcgctg gcgatcacgg ccgtggactc gacgacgatg
      541 agcctgggct ggacgcgcgg cgacggggcg aacgtgctgc tcgtcggccg ctctggcgcg
      601 gcggcggagg agccgacgga cggcacgacc tacgcggccg atccggcctg gggctcggcg
      661 ggtgcggtgg gcgcggcgtc gaaggccctg tacctggatt ccggcacgag cgaggacctg
      721 acggggctgt ccgcggagac gctctaccac ttcgccgcgt tcgagctcaa cgggtcgggc
      781 gggtcggaga actacctgac ggccggcgcg ccggtgacca acctctacac gctctccacg
      841 gagccctccg cgcacgcggg cagcttcgcg gcgacggcgg tttcgaagtc gcagatcgac
      901 ctgtcgtgga gcgcggcggc gggcgcggac gcgtacatga tcctcatccg gcagggcacg
      961 gaccccgcgg gcacgccgac ggacgggcgg gcctacgttg tcggcgacac gttcggcagc
     1021 gacacggtca ccgggctggt cgcggggacg tcgttctcgg cgtacaacct gtccccgaac
     1081 acgcagtaca acttctcgat catcccgtac aactgggacg cgagccacgc gcagacgtac
     1141 aactaccgga cggcggcgac gatcccgacg gcgaacgaca ccacctgggc gagggccagc
     1201 cgggtcatca aggaatcgtt caacagctac gcggacttca acaacctggc cggcgcgaac
     1261 ggcgggacgg ggtgggaggg cgcgtggtcc ggggcgtggg acgagggcga catctggatc
     1321 gacgacctga acttcagcgt gtcgctgcag ctgctgcccg cgcaggaggg cgactacacg
     1381 cgcaaggcga agttcgacgt cgactccaac ggcaagcgct cggcgatcaa gcggcagttc
     1441 gaggcgatca ccagcgggca gctgtacgcc atgtggatca tgcagggcgg ggagaacaac
     1501 gcctcccggt tctggggcgt cagcttcggc accaacgcca actacggctt ccaggccttc
     1561 ggcgtcggca agacccggaa cgacgggctg gacctcggca tctcgatccc gaacctgtac
     1621 agcaaccaga ccgtgcagac gacggattgg gacctgaacg cgggcaaccc gtacctcatc
     1681 gccatcaagt acgacttcga caccgacctg ctgcaggtca aggcctggta ctataccgtc
     1741 acggatatct tctcgatgca ggaacccggc ccgaccggcg tggccgacgg ctgggacatg
     1801 gagcggagca tcggcggcat cgacaagctc gattacctct tcctcgaggc gggctcggcg
     1861 gacgccggcg ggaatgccgg cgaatgtttc ttcgaccaca tccggatcgg caccaactgg
     1921 tacgagacca tggtcctggg cggggaggac gacccgtcgg ccccagtgcc gccgaccgcg
     1981 gcgctggtgt acatcgggac caattactcc gggtgggaca agagcctgac gaagaccaac
     2041 gtctacgacg gggaactcgc gaacacgaac gacccgatcg acttcgccgt gcggtggacg
     2101 gacgcgaccg gcatgttcgt gacgaacgac ccgtcctcgg tgcagaacat ttccggcacc
     2161 aaggggcgcg tctcgcccaa ctgggacccg ttgagcatcg gcgcggcgac caacgcattc
     2221 gggtttgacc gcgtcttcac caacttctac gggaacagcg gcgacggcag cgtcaccagc
     2281 taccagtaca gcgcgttcag cgtcaccaac ctggacatcg acgcctacac ctactacatc
     2341 accctcggcg gcgagaacga ggaccagagc agcggcgagt acacggccga cccgcccgcc
     2401 ggcggccagg acacgtacgg cagccgaggc atcctggtca acgagccgct gcggttctac
     2461 gtcttcgacg acgacaccaa cgcgccggcc ctcgcggaca tctactccgt gtccggcatc
     2521 gtggcgacgc tgcgcgacct ccacatcacc gtcggcacga gcaacaccta ctcgtccggc
     2581 gacacgacga accgcgtgtt ccggacggcg gacgggaacc tggcggccct gtccgattcc
     2641 aacccgttgc ggttgagcct gggcgccacc gacccgagcg gggtgtcgcg cggctccatg
     2701 agcaccagct ggaacatgtc ggtgaccatc ggctcggtca tccagtccaa caagaccgag
     2761 ttcagcgcag acgagagttc cccgctggcg gcggcgggcc ttacgacgaa tgtctggcga
     2821 ttcaacagcc tgacctacaa ccagatcggc gacctgttca cggcgcacac gaacaaggtt
     2881 gaggccacgg tgccggacgc cgacatggac cgcgtgaacg accagatggt gctgaccaac
     2941 cagcagtacg gcctcttcgc ggtctacgac gacgacgtgc tcggcccggc gctgggcgac
     3001 atcacggtcg tcggggccgc ggcctcgggc tccgcgatcc tgcagaccgg cttcgaagcg
     3061 tccgagggct ggccgatcca ggggagcgga gagccatggg acatccccat caccaacggc
     3121 ctggggaccg gcgtgtggca tggcacgggc tacgtcaaca tcggcgaccc gcgcagcggc
     3181 gtgcggaagg gcggcatgac ggtcgccggc gtgggccagt acttcgagct gcccgcgcgg
     3241 gacaaccccg gcaccctgtc cgtctacgcc cgcttgagca gcgcgggcgc cagccgcgag
     3301 ctggccgtcg agcggtggga cggcgcggag tggaccggct gcggcaccaa cacggtcacc
     3361 agcgacagct accagcggta cgactgggac gtcgactgga tcgagaacgg cgtgaccctg
     3421 cgcctggtgc gggtcgggac cgacggctcg cccgggatct tcctcgacga cctggagctc
     3481 atctccgccg acccgatctg gttgagcgcg acggcgatca cgtcgttctg gaacgccgca
     3541 gtggacgcct ccggcgtgta cgagtaccgc gctgtcgcgc cgtcctacag cgcctccgag
     3601 ccgtcacaga tgacggacgg gacgtcgctt ggcgcgggca cccagaacgt cttcaacgtc
     3661 gccggctgcc agggcgtgct gacgggtttc gtgttctccg tcgacgacga caacgaccgc
     3721 gccaacgacc gcaccaacgg caacaattcc gccttcctcg tgcggatcga caccaacccg
     3781 ccgccggtga tcgccaacgt gacggccatc gagggcgagg acccgacctc cgaggccatc
     3841 gtgcagtgga ccgcggcgac gaacgcgggc gagcgggccg cgccctccta cgccgtgctc
     3901 tcggcgtggg atacctaccg ggtctactac accgacgacg agaccgagcc caccacgggc
     3961 agcccgtacg tggacaagac ctacgacgcg gccctgacca acatgtccgt caccaacgtg
     4021 acggtcacga acctgatctt cgacacgacc tacaagttcc gcgtcgccgg ccgcgaccag
     4081 gcgggcaacc tcggcccgct gtccgacacc ttcaccatcg tgctgcccgg cttcaacgtc
     4141 acccagggca tggtccaggt cggctccggc ggcatccgct tcccggagat ctactggaag
     4201 gccgcgacca acgccagcgg cggcgtcagc cgggaatacg acctgctgtt cgcggacgcg
     4261 atcagcttct ccgacagcct gtccaaccag tgggcgctgg tcgaaagcgg atggacgaac
     4321 gcgctgccgg acaccggcga cgtcgcccgc gccgcgccga cgctgatggt ggacaccatg
     4381 cggttcttcc gcgcggcgtc cgcggaccgc tggctgatcg accggtcccc gcgcgtcgcg
     4441 agcgaagagg tctacggcat gaagaccgtc tggctctatc ctggccagaa ctggatcggg
     4501 ctctcggtga tcccggacag caacaccctg cagaacgtcc tcgggcacga gctgccggcc
     4561 ggctcgatgt acaccgaccc ggacagcacc atcgtcagtt ggtacaaccg cgccagcaac
     4621 gaggtcgtga agaaggacgt ctggctgctg gactggggcg cgtcgaacca gtggcggacg
     4681 ggcacggggt ggggcggcct gaaccagccg gccgactacc gatcgctgcc gaccgacgag
     4741 ggcttcgtgg ttgtcatccc gacgaacacc agcgagaccc ggctgatgat ccatctcggc
     4801 cgcgtgccga cgaacatcca ggaacaagtg atcgagccgc acgcgtataa cctggtcaac
     4861 atgcgcgtgc ccgcgaagct gaaaccgagc cagatgaacc tggtcgaatc cgggttccag
     4921 ggcgggccca actccttgat ctccgaccgc ctgcgcaagc tcaaccgcga gctgaagagc
     4981 acgggccggg acgtctggta caatacgacc gaacagaaat ggtactatgc gggcgcgggg
     5041 ctggccgacg acttctacat caccccggac gacgggttcc tcatctggac ccgcgcctcg
     5101 gcctcggcct ggacctggac caacgcgctg ccctacagcg cgccgacccg gctgatgaac
     5161 ccgtagcagg cggatggaat gtttttgccc ggggcgggcg gctgatctag catccgtcag
     5221 gatgatggct ccctgtgaca agcgtccgcg cggccaccct tcgcaggcgc cagcggcgga
     5281 cgctcgtttg aacgaggaat ggaaagaaga acggtccggc gggagcagga ggcgctggtc
     5341 cccgcgcctg ccgttcgccg ggttgctgat tctctgcggg acgctggcct atcgcccggc
     5401 gctgacgcgg attttcgaat ccgatcagct tggctatttc gcggaactcc aggggcagac
     5461 gacgctggct tcgggcttga gcttgctcga ttacggcgcg cgccggcagt atttcaaggg
     5521 cgaccaggtg ctctaccggc cgctcctgtt cgccggcctg gccctcgaga acgccgcgtt
     5581 ccggcgcgat ttccggttgt ggaacgcggc caacctgggc gtgcacctgg ccgtgtccta
     5641 cctgctgttt gagatcctgt ggcgcacacg gcgatcggtc ctggcctgcg ggttcgccct
     5701 ctggtttgcg ctgttgatgt cgaatttcga actggtcacc tggagccacc tgggcggcta
     5761 catgctggga tatggcctgc ttctcctggc cctgcatgcc cggggccaga tggagatgga
     5821 ccaggccggc gcgcgatggt tctgggtata cgggttctcc atgatcggcg cgatgctggc
     5881 gcatgaaatc gcggtcatcg ccggcgtcgg catggccgcc cacgggtttt ggaccttcgg
     5941 ccggcggccg gacgccgggc gccggcggtg ggtcgcgtcc ctgctggcgc ccatcctcgt
     6001 ctacgcggcg ctctacgcgc gccacgcgct tcggtgcgat cggtttttct ggtggtctgc
     6061 gcccgccgga gcctccgtcg cggcgggcct ggcggacctg ccttccttcc tttcaggctg
     6121 ggcgcagcac atcctattgc cgcgccagga ccagttgatc tgcagcgtcg gatcgcgttc
     6181 ggcatgggcg gactggccgg ggggattgcc gccggcgact cttttggccg gcgccttgtg
     6241 ggcggggttg ctggccgtcc tgtggccggg cctgcggtct caacgaaacg ggtccggctg
     6301 gccttttgga aggttcgtgg ccttcctcgt ggcggcctat gcggccatga acctgctcgg
     6361 ccgcccggcc tatgccatgc aagtcccgta ctatgactat tttccggcgc tgctgggcct
     6421 ggcgtggctg tgttcccggg tcgatgttac tctcgttggc cgaaaaagga tgatcggcgc
     6481 gtgcgccggc ctgctcctgc tggcggccgt caacggatgg caggtgcggc agatcggcaa
     6541 ccggctgcac gcgatgcacc gcccctgggc gcaatacctg ggctgggtgg aggccacggt
     6601 caggccccgg ctctccatcc ccggctattc ctttgatgtc cgggggacgc ccgcggacat
     6661 gaatctggag ggagacctgg cgatcggatt tccggaccag aatcaggtcg tgattagaaa
     6721 cgtgctcact atactgtacg gaaactttca taatgtcgcg gcgccggcgg aggtccttgt
     6781 atttccgggc ctggatgcgg ggccgcgttg atgaagagcg atatgttctc gcgggctgca
     6841 caagaatgga tctccggcag gaagggccgc tgcgcgctgg ccggcctggc ggcgatggcg
     6901 gcggccttcg tgctgtatgc cccgagcatc gggcacgatt tcagcctcgt ggacgaccgg
     6961 tatttcgtgg aggacaaccc gctggtccag ggcgggctga cctggccggg catccgttcg
     7021 gcctggacgg cggtccatgc cggctactgg gcgccgctgc tctggatcag cttcatgctg
     7081 gacatggaga tttccggcgg gtcgccgtgg agtttccatc tcgcgaacgt cgtcctcttc
     7141 tcgctgaacg ccggcctggt cttcctgctg atccgccgct ggacggggcg cggcggcctg
     7201 gccctggcgg cggcgctgct ctgggcgttc catcccgcgc gggtcgagag cgtggcctgg
     7261 atcacggagc ggaaggacgt gttgagcggg ctgttcttcc tgctcgggct ctggttctac
     7321 accgcctccc gccaagcctc ctcctctccc tcgtgcatct tcctctcctg gttctgcatg
     7381 ctcctcggcg gcatggccaa gcaggtggtc ctcgtgatgc cggtcgccct ggtcttgctg
     7441 gacatctggc cgctgggccg cacggactgg aatcgcttct ggaaagacgc cgggcggctg
     7501 gtcgcggaga agtgggcctt ctggttgctg ggcttgctcc tggccggcat ggccctccgg
     7561 gcgcaggcgg gggatcaggc cctcatcccg gtctccgccg gataccgcct cgcgatgatt
     7621 cccgtgcatt acctgtttta cctcgtgaaa ttggtctggc cggccggcct ggcgcccctg
     7681 caggacgatc tcctgtggag cgggtggaaa atggccgctg gattgggcgc gctggccggg
     7741 gtcacggcgc tgctgtggaa gcttcgcgtc aaggccccgt gggccttgtg gggctggatg
     7801 tggttcgtgg cgttgctgct cccgttgagc ggcgtcgtgt ggtcaggatc ggagcgcgtg
     7861 gccgtccggt tcctttacct cccgcagatc ggccttacgc tggccgtggt cctggcaatg
     7921 gacgccctgg ggcgcgcgcg cggatcgagc gggggctggc tccgggtgct gctcgcggtc
     7981 gttctcctgt tcagcggcat cgccacgctg cggaccttgt cgcattggcg cgacagggac
     8041 tccttcggcc tgtggatctg ggagtgccat ccgcagcagg gcggcgcctg cgcgatgggg
     8101 ggcgacaccc tcatggcctg gggcgagtgg gccgaggcgc tgaaggcctt cgagcagggg
     8161 gcgtcggtcg gggacaaggc ctgtttcctg cggcagggca tggtctggaa ccacctgggc
     8221 cagatggagc gcacggcggc ggcctgggtc gagttcgaac ggaagtgggg cctcccgctg
     8281 ctgggcttcg ccgattggga gccggccccg gagcgggaac tggcttggag cgtgcgcggg
     8341 caggtcctgc tcgcccgggg cgaccatgcc ggcgcgatcg cggcgctgaa ggaagccgtc
     8401 cggtgggagc ccgatcccgc ggccctggtc gtggcggact accttcgcgc ctgccacgag
     8461 gccggccgcc cggaggaggg cgccgaggcg gccgagcgcc tggcggccgc gggggacgtc
     8521 cgcgtccgcg cgtggcgcga cctgttcccg ttttacgcgg agatgtggaa gcggggggcc
     8581 cgcgggtatg cgtatggtta cctggccgag tacgcgaaac ggtttcccgg ggacgccgtc
     8641 aacctgaact acatggcctg gctgctggcc acggccatgc cggacggcct ggcccacgcg
     8701 cggatggagg agtggccgca ggccgcggtg cgctgggcgg aactcgcgct cgcatcgggc
     8761 gacccgtcgc tccccggcgt gtgggacgtg ctggccgccg cgcgcgccaa cgcgggcgat
     8821 tccacgggcg ccgtccgcgc ggcggagaag gcgcgggacc tggccaagga gaaaaaggat
     8881 gacgccttgg caacggatat cgaaaagcgc atcctgaaat accggatggg cctggcgtgg
     8941 cgggaatgat cgcggccgaa ggaatgctta tttcccgtcg tcctcgttct cgtcccccgt
     9001 actcgacaag agcggggggg ggtgagatcg aggacgatgg acgaggagga gaccgggaac
     9061 gatgggggag gattgcggcc gaataaacgc gaatttccgg tcgtcctcgt tatcgtccgt
     9121 cgtactcgac aagagcaggg gatgagatcg ggaacgatgg acgaggagga gtacgaggac
     9181 gagaggaaag gaaacctccg tgcaaaacga gttggaccac gagaaccttg atgtctacca
     9241 ggtcgaactg cggttcgtcg aatggtcaac ggatttgatg gtggaactgg tcgagtctgc
     9301 cgaggcccgg gggcgcagga ttgcggaggt ctgtgatcac ctggaccggg ccggtctttc
     9361 ctctcttttc aatacggcgg agggcaatgg caagcggccg atgaaaaccc gggccaagtt
     9421 cttcgatgat gcccggggtt cggccacgga atgcgcggcg tgcctcgacg cgttggtcgc
     9481 gaaacacgct tgttccgaaa aacgtgttga gcagggaaag atgctgctgg tccggattgt
     9541 tgccatgctc acaaaattgg tggatcgttt ctctgtaacg gatcgggtga tggaagccgg
     9601 aatggagtat gaggtcgagg acgagaacga ggacgaggaa gaggacgaga acgagggagt
     9661 gaaggagcgc ggggacgagc aagaggcatg acggcgaaag acatgagagc ggataaacgc
     9721 aagctggctg aaccgacgcg cgatcagccc cgtcgtcttt tcagcgggcg cgggcgcgcc
     9781 gccggggcgt gcctcctggc gatggcggcc gcctttgcgc tgtacgcgcc gaccatcggc
     9841 tacgatttcg tcaccctgga cgacccgatc tacgtggaga ccagcccggt ggtcggggac
     9901 ggcttctcct ggaagggcct ccggctggcg tggaccacgg cgccggagaa ttactgggcg
     9961 ccgctgctct ggatgagctt catgctggac atggagatct ccggcgggcc gccgtggagc
    10021 tgccacctga ccaacgtgat cctcttctcg ctgaacgtcg gcctgctctt cctgctgatc
    10081 cggcgctgga cgggccgcgt gggcctggcg ctggcgacga cgctgctctg ggccttccac
    10141 ccgacgcgcg tggagagcgt ggcctggatc accgagcgga aggacgtgtt gagcggggtt
    10201 ttcatcctcc tcggtttctg gttctacacc gcctcccgcc aatcctcctc ctcttcctct
    10261 tcctcgtcct cctcctcgtc ctcgtacatc ctcctctcct ggctctgcat gctcctcggg
    10321 ggcatggcca agcaggtcgt catcgtgatg ccggtggcct tcctgctgct ggacatctgg
    10381 ccgctgggcc gcacggactg gagtcgcttc tggaaggacg cggggcgact gatcgcggag
    10441 aagtgggcgt tctggctcct ggcgatcggg ttcgcctcgc tgcccatcgc gttccacgtg
    10501 gcgcggaagg ccgtggtgag cgtgcccgtg ctgcaccgcg tggggatgat ccccgtccac
    10561 taccttttct acctggccaa gctggcctgg ccgacgcggc tgatgccgct gcagccggac
    10621 ctgccgtcgt ggtgggaact cctcgccggg gcgttctgcg tgctggcggg cctgacctgg
    10681 ggggcgtggc gcctgcgcca ccggttcccc ctcgcgctct ggggctggct ctggttcgcg
    10741 ggcctgctct tcccgttgag cggcgtgatc tgggccgggg cggagcgggt ggctacgcgg
    10801 ttcatgtacc tgccgcagat cggcctgacg ctggccgcgg cgtgggcggc ggcggactgg
    10861 attcgttcgc gcggctggag cgcgcggcgg ggcgccgccg cctgcgcgct ggtcctggcc
    10921 gtgtggggcg ggatgtcgct ggccctgtcg cggcactggc ggagccgcga ccatttcggc
    10981 atctgggtat ggacgtacaa cccgggccac ccggtcgcct gcatgctcgg cggggatggc
    11041 cacctgtccc agaagaactg ggcgcaggcg atgcgcgcat acgaggccgg cagcgcgttc
    11101 tacgacaccc attgcttcct gcgcctgtgc atgatctgga actgctgcgg ctggccgaac
    11161 ctgaccgagg acgcctggcc ccggttcgag aaggggctcg gcaagaccct gatggaagcc
    11221 gtccgggacc agcggcccct ggagcggatt ttcatctggc gcatccgcgg gcaatgcatg
    11281 caggcgcggg gcgacctcga cggcgcgttc ggcgcgttca aggaggccgt cgccctcgaa
    11341 aaggacccga cggccttcgc ggtggcggaa tacctccgcg tctgccacga gaccggccgc
    11401 acgaacgagg cggcggaagc agccgagcgc atgaccaagg cggcgggcaa gccccggcgc
    11461 acctggggcg acctgttccc ctgctatgcg cagatctggc tggacggggc gcggggctac
    11521 gcctacggct acttcctcga gtacgtgaag cggcatcccc gggacgccgt ggcgttcaac
    11581 aacatggcgt ggctgctcgc cacggccaag ccggtcgatg tcaaccacgc cagcatggag
    11641 gagtggcccg cgaaggcggt ggaatgggcg gcgcgcgcgg tcgagctgga cggcgggaag
    11701 gccgccacgg cgtgggacac gctcggcgcg gcgcgcgcga acgccggcga cttccccggg
    11761 gcgatccagg cggcgaaacg cggcgccgaa ctcgcgcggg aggggggcgg cctggacctg
    11821 gcggcgcaga tcgaggcccg gctggcatcg tatcgggcgg ggaaagcctg gatcgagtag
    11881 gcgtccgtcc ggagtgcagc aggccgccat atcgctgcct ttgtagtgct ttggcccaat
    11941 agggtcaacg gcgtccaagc cggcctggaa cggcctccaa gacaccccgt tcaatttcga
    12001 catacggaat ctccttattg atagcaaatt gtgacgatgc gatggcgctg cgagccgttc
    12061 ttcgcgccgg cgccggcgcc gggtctccgc agggcctgaa accgcgtcgc gccggtccac
    12121 aatccattga cgcccaacgg cattctaggt attaatacta gtaagtaaaa aagggatttc
    12181 cctcggatcg gcagggagaa ctcctttatg cgtgagctgt ctccttatgg ggacggtaat
    12241 gtatttctgt caggaaaaga gccgatgagt tgatcagccg gaaatggacc ggaagccgtt
    12301 gttctagtcc aagggggcga cgggcgttcg cgagcccgga ggagtgaaaa gccatgaagc
    12361 atgaaacgat gtcgaagcct gtcgcccggc gcggctggcg gccgttggcg ctggcgatcg
    12421 cagcggcggc ctggtgcctc gcgccgatgc ggtccgaggc cgcctactcc gggtcgggga
    12481 cgttcaccct gatcacgtcc acggccgaca tcacctccgg cggctactac gtcttcgtcg
    12541 gcggcggcac cgccaacgcg atggcgtctt cggcgctcgc gacggtatcc acggcagccc
    12601 tctccgccca ggcggtcacc atcaccgcca gcgccattgt ggacccggcc gtcgggcgcg
    12661 tgtggagaat cgatgcggtt tcgagcagct atggcatcta caatgaggct ggcagcatct
    12721 acatcggcgc ccccgccgcc aacggcatga tcagatccgc ttccgccagc tctgcgacaa
    12781 tctggcactg gacgttcgcc accactgcgt ccagccggtt cttgattcaa aatgtgggcg
    12841 ccgcgtctgc gcgcaaactg cagtacaact cgagctcgcc ccggtttgcc gcctattcca
    12901 gcgcctcaac gtatgttgag ctgtacaaga tgaacacctg cgagaacctc tccgcggtca
    12961 cgaatctcca gcagcagagc cagtacgaca cgcggtggga tagcggcgcc ggcacgttcg
    13021 acacgggggg gagcaccgaa ctgggcatgt gggcgggcgg cgaccagggc tacacggtgg
    13081 cttggaagac gttccgcacg gccagcgcga cgacctcctc cgcgcgcgag ctgcaggtgg
    13141 gcgacgagtt cagcatcacc gtgtacagct acggctgcta ctacggcgag atcggcgtgt
    13201 cgctgaacga cggcggctcg accggcacgc cgtgggcgaa ccgggtcagc gggtcgcgac
    13261 tgtccgtccg tcaggacggc gggaactacg ggtcgggcgg gggcatcggg tcgtggtacg
    13321 cgatcggcag cggcgcgggt gaatcgttca cgaacacgcc ggcgggctcg tcgggggcgg
    13381 actacacggt caaggtcaag gtcacgtcga gctcgacgtt caacgtggac ctgaacggga
    13441 agacgaaata cgactggacg atggcgggtt cgccggcgac gtcggcccgc atcgacgcct
    13501 actcgatctt cctgaatgac gaccggcggt accagtggga ctggggtgac aaccgccaga
    13561 actcctactg gaagcagacg gccaccgtgc gagacaccgg cgcggtggag ttcggcgggg
    13621 gcaacggcac gtccacgatc aacggcctga tcacggacgg cctgggcgcg gcctgcgcgt
    13681 cgggcacgac ggccaaccgc ctctacaaga tcggcacggg cacgatcacg ctgggctgca
    13741 cgaccaacac ctacacgggc ggttcggcgg cggaaaacgg catcctgcag ggctcggcgg
    13801 acctgtgctt cggcgcggcg cccgcctcgc tggtgaccaa ccacttcgag gtctggtcga
    13861 gcggcacgct gcagttcacg ggatccttcg ccctgaacgc caaccgcggc atacggctgg
    13921 gggacgtggc cacgccgaag ctgggcgtga ccagcgggaa cgcggtgagc tacggcggca
    13981 gcatggtggg gacggcgaac tggcagaaag tggagtcggg aatgctgacg ctgacggggt
    14041 tcaacaccaa ctcgggcgtg gcgcacatta cggcgggcat cctggcgttc ggggcggacc
    14101 tggcggcggg cccggtgccg ggcggcgcgg gggagaagat caacgtctgg agcacgggca
    14161 cgctgggctt cacgaacgtc ttcacgttgc acgcgaaccg gaccatcgag ctgggaacca
    14221 ccgccggccc gcggatctgg gtggcgccgg acagcacggc gacggtcaac ggcgtgatcc
    14281 agaactcggc ggcctggacg aacatcggaa agggcacgct gatcctggcg ggcaacaaca
    14341 ccttcagcgg caacctctcc atcacgcagg gcgtggtgcg ggtgacgaac gcgaacggcc
    14401 tgggcggaac gggcgggaac acctacgtcg ccaactcgac cgccagcgcg ctggagctgg
    14461 cgggcgggat cacgacggcg gcggagccgc tgttcctgaa cggcacgggc atcgccagcg
    14521 gcggcgcgct gcggaacatc agcggcaaca acaccttcgc cggcgcgatc acgctgcaga
    14581 gcacgggcgt gcggatcaat tccgactcgg gcatcctgac gctgaactcc gcgacggcca
    14641 tttccggcag cggctacaac ctcaccttcg gcggcgcctc gaacatcgtg gtcagcagcg
    14701 ccatcgggac cggggcgggg accgtgacca aggacggggc gggcctgctg accctgaccg
    14761 gctacaacac gttcagcggc gcgctgacga tcagcgcggg cgcggtgcag ctcggcgcca
    14821 acggcacggc cggcgccgtg acggccgcca gcatcgtcaa caactcggcc ctcgcgttct
    14881 accgcacgga cgacacgaac tacctcggcg tcatcagcgg caccggctac gtgacgaacc
    14941 ggggcgccgg gatggtgctg ttcaccaaca acaacagcta cagcggcggg accgtgatcg
    15001 acgc
//
LOCUS       DAOOTK010000083        14719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2758_length_14719_cov_6.192580, whole genome shotgun sequence.
ACCESSION   DAOOTK010000083 DAOOTK010000000
VERSION     DAOOTK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14719
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_14719_cov_6.192580"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PK634_09660"
     CDS             <1..1318
                     /locus_tag="PK634_09660"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOW98042.1"
                     /translation="FSAHRGLPASRTRSWMALVAGGAELRLEARTRNLSIGGHLLGEG
                     AWLSLDGFTGAIYQGEVPCEPTAPAAAVAEGRKTEQKAFPVRMYRQLSEWTDRLRKME
                     VRAAVLGPRDARAARSLGADGIVYSPGPMFLGKEPLRLIREYLWAEDPTPRRRALDHL
                     HALCRAEMEKLLEVADGRPVCVRLLDMAPDDWLSLRVGELAALARRLGISAEKARERQ
                     KRLSESRPFEGVSGCRLLMGCPDLGAMQAAAIVEAVCNQEKRGLKVQPEIAIPRIAGA
                     AEFDLCARVVRETAARVLKERKMRLKLPIGAVIDTPRAALTADHLAESAEFFLFDGDE
                     LTRAVFGLPRRTVTPAGLDPTERKPLPSDPFPALDTGGVGQLIELALRKGRETRPDLA
                     CGICGELGGDPNSVKFCFKIGLNYVSGSPYRVPVARLAAAQAAITQ"
     gene            1434..2288
                     /locus_tag="PK634_09665"
     CDS             1434..2288
                     /locus_tag="PK634_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOW98043.1"
                     /translation="MEGEDRGIKIYLREIGQTALLTREDEVKLARRIRRGDEEARQLM
                     IRANLRLVVKIAHDYAQYGLPLLDLISEGNIGLMKAVERFNPRKGGKLSTYAAWWIKQ
                     SIKRALANQSKTIRLPAHLVDKIARMRRAERNFAQKKGRDPTEPELAKAVGVSVATIR
                     MWKTVSLRPASLNAPIGDEDSAEYGDIIGDDRNVSPVEDLSDRQLKGEIEGLLDRLSK
                     REREILMYRYGLRGVKEETLEVVGRRFKITRERVRQIQNSAVLRLRQMLEDMDQPQDS
                     AEPTEKYP"
     gene            2285..2809
                     /locus_tag="PK634_09670"
     CDS             2285..2809
                     /locus_tag="PK634_09670"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOW98044.1"
                     /translation="MTIPALKNAIRDIPDFPKEGIIFKDITPLLAEPSLFKLAVDTLA
                     GRHRDSRIDRVCVVESRGFLFGSAVAYQLGAGLVPIRKKGKLPHETLEAAYDLEYGTA
                     TLQVHVDAFEPGTRVLLIDDLLATGGTAEASARLIEQLGGKLIEIQFLIELAFLNGRE
                     KLTKYPVFAPIVYD"
     gene            2913..4109
                     /locus_tag="PK634_09675"
     CDS             2913..4109
                     /locus_tag="PK634_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98045.1"
                     /translation="MNRVAIICLALSVLARVGRAQSPTIDGVLDADFYGAARSVQDTP
                     TSFGDATNGHIRFARGGSELDAAYARISDGTLFLFIAGNVETAGQGIQWPVGNRNKLD
                     IFIDSIEGGQSSLRGDNADVDGNGLNRMGHLDPSNDGLKFDTGVAHDFFLTFNNYTEV
                     VFIPGSGNQELWRGQLYYASLPTGGSGTGTLVGTASDSWPGSYPDSFTLSQGVRMGFR
                     NSNTNGVTGTGAPSPSAADAPNVTSGLELAIPLALIDSTNGDLNAEIRITAFINDSAH
                     GYLSNQVLGPMGQPSGSYGNLAEPRVFDFSQSYSPGAQYFTADNAYPSTRLILPVTLL
                     DTGSISNRWIGEVGATYVLQYTTNLLTGPWTNVSGVVTAESPLVSFVDTNSAASRMYY
                     RTARLP"
     gene            4168..6705
                     /gene="hrpB"
                     /locus_tag="PK634_09680"
     CDS             4168..6705
                     /gene="hrpB"
                     /locus_tag="PK634_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOW98046.1"
                     /translation="MDRRELPIFEIERELLARAAEHRRLIVRAPTGSGKSTQIPQMLL
                     DAGLLKSGRAVILQPRRLPTRLLAARVAAERGAKLGAEVGYQIRFENIAGRDTRILYE
                     TEGVLLRQMLSHPRLDGVAAILFDEFHERHLYGDVTLARALQLQRAARPDLLLVVMSA
                     TLPSGPLDEYLAPCAALEAHGRLFPVITEYLDRPADFDRTPPWEAAAAEFERVCREGE
                     PSDTLVFMPGAYEISRTVQALENSPAARGRIILPLHGELPTADQDAAVARYDRPKIIV
                     ATNVAESSLTIEGVRLVVDSGLARIPRFDPARGINTLLVEKISRASADQRAGRAGRTA
                     PGRCVRLWTEREHAERPVQEVPEIRRLDLSEVMLTLRAAGAGDLRELPWLEPPGVRAL
                     DRAEMLLRDLGALDEKQAVTELGRRMAGFPVHPRYARMMIAAGEHGCVRAVALIAALT
                     QGRSLWMRKPDKSTLEQREDVLGEEGASDFFGLMRAFRFADSRRYDVGACNRLGIHAN
                     TARQVRALAGFFLDLAEKTGLKIEARAPDTDAICKCILTGFSDQVAKRLDAGTLRCQL
                     VHGRKGVLARESAVQGPLLVVSEVREVEGRGGELSVILSLATAIRAEWLEELFPGDFS
                     EPVDVEYDAAERRVVAWRRRMFRDLPLESKRVDPPPADAAGDLLAEEVINGRIKLPHW
                     DETTDQWIARVNSLAAWCPELGLTAYSEEDRWSVLRVLCRGAVSARDLKDRPVTAVFK
                     DQMDPARRALVEKHAPERIELSNGRRARLTYTEGEPPHIAQRIQDLYDVKETPRIAKG
                     RVPVVVEILGPNHRPVQVTRDLAGFWRDHYPRVKKEMQRKYPKHEWR"
     gene            6702..7313
                     /gene="trmB"
                     /locus_tag="PK634_09685"
     CDS             6702..7313
                     /gene="trmB"
                     /locus_tag="PK634_09685"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOW98047.1"
                     /translation="MKNPFSEAFRIVPDDWLNPLPLERWFRPSAPLEVDVGCGKGRFL
                     LARAAAHPDTDFLGVDRMIGRLHKIDRKLVRRGLSNARWLRLDAYYAVACMIPPEAVT
                     TYYIFFPDPWPKKRHHDHRLFNAAFLDALFRTLKPGGQVHFATDHLPYFDEVVAAIKT
                     DPRFAEIPAFEPAEDERTDFELIYLGQTPIGRYSFEKRRGGAL"
     gene            7310..8182
                     /locus_tag="PK634_09690"
     CDS             7310..8182
                     /locus_tag="PK634_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOW98048.1"
                     /translation="MNDQNVMMMFSIGALAMTALALAYGAHGHRHVRPRWVCLVIANL
                     LLGLWLVAVGLLAGEVYFRHFLDMTDSFAISRVHQAWLHRHWTLNKQGIRDTLQSYTL
                     KPAPGKERIVFLGDSFTAGHGIRNVDHRFANLYRAMHAEKEVHVLAHSGWDTGDFLNC
                     LQDFKRQGYVFGEVVLISGLNDISDIDPAWQRMTAQVKDSFKPSGILQNSYLLNWLYF
                     RMKVVTSPAFRDYGLGVQERVRGPGVERTENEDAYTTRHRPVSRGPIGGRHASLFQLP
                     GRNISFHGDSRATG"
     gene            complement(8415..10133)
                     /gene="ade"
                     /locus_tag="PK634_09695"
     CDS             complement(8415..10133)
                     /gene="ade"
                     /locus_tag="PK634_09695"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOW98049.1"
                     /translation="MNAEMIAAARGETPADLLLKNARVVNVFSNEIHPADVAVFKGHV
                     IGFGKYEARETVDLQGRLLCPGFIDAHVHIESSMVTVPEFARAVVPRGVTTVVTDPHE
                     IANVLGLDGVRYMIETSEGLPLRVFVMVPSCVPATDMETSGARLTAEDFSLLFQHPRV
                     IGLAEMMNYPGVIYRAPDVLAKLTAAEGHPIDGHAPGLSGKDLAAYIVAGIGSDHECT
                     TLEEAREKRQMGMHIFIRDGTTARNLDALLPLVTPAAAINCSFCTDDRHPADLLREGS
                     INWLVHRAIQKGMDPVTAVRMGTLHPARYFGLKKLGAVAPGYWADLVVIDDLESFRVA
                     QVYQAGQKVAENQALLAPRAKAKPHRLRSSVNVQWHGLDLRVAVPEGKADRPRIRVIG
                     LVPDQIVTKLLEDEAAVADGAVVADPARDLLKIAVIERHQASGHVGIGFVRGFGMKSG
                     AIASSVAHDSHNIVVVGATDQDMMAAIREVARLRGGQAVVNGEQVLASVPLPIAGLMS
                     DLPLEEVRDRIEAMAAAAHGLGCTLPDPVMTMSFLALPVIPELKLTDKGLVDVTKFQP
                     VSLFVE"
     gene            complement(10219..12546)
                     /locus_tag="PK634_09700"
     CDS             complement(10219..12546)
                     /locus_tag="PK634_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOW98050.1"
                     /translation="MAKKNFKVVNQPVKKLDALPLALGKPLFVADFKPADALYAKLLW
                     SPHAHAIIKSIDISAAEKMPGVHCVLYHGNVPRIAHTTAGQGFPEPSPYDTFMFDRKV
                     RYVGDRVAAVAAETPEIAEAAVKAIKVEYERLEPVLSIEAALKEGSPVIHDEPEARAV
                     IPVPYEPKKNIAARVGMDVGNVEQAMAGAPYKLDNSYYIHMAQHCPIEPHVTMAHVNA
                     EGRLVIRTSTQVPFHARRIAAQTMDIPVQQVRVIKPRIGGGFGAKQEVLLEDVVGMLA
                     WRSRRPVLLMYTREEEFVSSRTRHPERIRVRLAADPEGTLTAVGLDCISNTGAYGAHA
                     LTVVCNSGSKVLPLYRAPNVHFTGTAVYTNLPVGGAYRGYGATQAAFAVESAVDELAE
                     QIGVDPLVLRSNHHIRSGETSPVFAALGEGKAGVEQAIGSCGLDKCIRLGAKAIGWKK
                     RGDWRKKKGRYRRGIGMCCLMQGSSIPEIDMGAASLKMNEDGSFNLLMGATDLGTGSD
                     TVLAQIAAEAIGTTSDKILVYSSDTDMTPFDVGAYASSTTYLTGNAVLDAGLKIKKQI
                     LGVAAEMMEKKPADLEIRDAQVFARDGSGSVTFQDVALHSLYAANQFQIAAISSHISH
                     KSPPPFAAHFAEVEVDTETGLIRVVKYVAATDCGTAIHPKLAEGQVEGAVMNGICYAL
                     TEQYLFNSAGRMLNDSFLHYKIYSMRDKPEMVTILVPTYEKTGPFGAKSVSEIGINGP
                     CPAIANAIYNAVGVRLREAPFTPEKIWRGLQQHNA"
     gene            complement(12567..13049)
                     /locus_tag="PK634_09705"
     CDS             complement(12567..13049)
                     /locus_tag="PK634_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOW98051.1"
                     /translation="MSTTRTITFTLNGKKRDFQIAPGELLVKLLRRSGLTGTKEGCDE
                     GTCGACTVNVDGRAVLSCIMPAFLAHGRNVVTIEGVGDFDRVHPIQQALVDAGAVQCG
                     YCTPGLVMSAKAMFEENPAASDEDIRVHTDGNLCRCTGYEKIWTALRNVRDANKPAQA
                     "
     gene            complement(13046..13891)
                     /locus_tag="PK634_09710"
     CDS             complement(13046..13891)
                     /locus_tag="PK634_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOW98052.1"
                     /translation="MKITDFKIPTRLDEALAALRDLGPAALPVAGATSLVFIPEDQPM
                     TAVDLNRLGLDGIRRDGDAFRIGAATRVAALQKRHEPGWVLDRVALTLSSQQIRNEST
                     LGGNICRVFPWSDFPAALLALGAGLVIRGAQEKEMTADEFFSSQPARLFRGGDLLTHI
                     RVPAVGAGAGFGYKKVRQTATGFSLATAAACLQLDGGGIRSARAALSAAVPFPARCAA
                     VEAALAGKKASEGLLREAALNGLSGLKFKEAEGMSAEYIGRLAAVTLGDALVEAWAQA
                     KEQAS"
     gene            complement(14015..>14719)
                     /locus_tag="PK634_09715"
     CDS             complement(14015..>14719)
                     /locus_tag="PK634_09715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOW98053.1"
                     /translation="ILPLFHKIEPLESPEIVAAAEDLARQSGVRVVGVFRWGLEEKTE
                     AANAALAGLGRTRRILLGDTLLKGYGRDEILAILAHELGHYRHSDIARLITAGTVLAA
                     LGFFIAQAALKGLVVAFRFGGPDDIGSFPLLVFCLFLFSLVAMPFSNAYSRRRELAAD
                     EYAVRSTGSAAPLVSSLNKLAAQNLAVRRPAAWLEFLLYSHPSIARRIERARLVEARL
                     PGAKQPPHDAPRARAP"
BASE COUNT         2370 a         4945 c         4993 g         2411 t
ORIGIN      
        1 gttctcggcc cacaggggct tgccggcctc gcgcaccaga tcgtggatgg cgttggtggc
       61 gggcggggcc gagctgcgtc tcgaggcgcg gacacggaac ctctccatcg gcggacacct
      121 gctgggcgag ggggcctggt tgagcctgga cggcttcacc ggggcgatct accagggcga
      181 ggtgccgtgc gaaccgaccg cgccggccgc agcggttgcc gaggggagga agaccgagca
      241 gaaagcgttt ccggtccgca tgtaccgcca actgtcggaa tggacggacc gcctccggaa
      301 aatggaggtg cgcgcggcgg tcctgggccc gcgcgacgcg cgcgccgccc ggagcctcgg
      361 cgcggacgga atcgtctatt ctccggggcc catgttcctc gggaaggaac cgctccggct
      421 gatccgggag tacctgtggg cggaggatcc gacgccccgt cgccgggcgc tcgatcatct
      481 tcatgcgctc tgccgggccg agatggagaa gctgctcgag gtggccgacg gccggccggt
      541 gtgcgtgcgc ctgctggaca tggccccgga cgactggctg tcgctgcggg tcggcgaact
      601 ggcggccctg gcccggcggc tcggaatatc cgcggagaag gcccgcgagc ggcagaagcg
      661 tttatcggaa tcgcggccgt tcgaaggggt cagcggatgc cgcctgctga tgggctgccc
      721 ggaccttggc gcgatgcagg ccgccgcgat cgtcgaggcc gtgtgcaacc aggagaagcg
      781 cggcctcaag gtccagccgg agatcgccat cccgcgcatc gcgggcgcgg ctgaattcga
      841 cctgtgcgcc cgggtggtgc gcgagacggc ggcccgcgtg ctcaaggagc ggaagatgcg
      901 gctcaagttg cccatcggcg ccgtaatcga cacgccgcgc gcggcgctca cggccgacca
      961 tctcgcggag agcgcggaat ttttcctgtt tgacggcgac gaactcacgc gggccgtctt
     1021 cggcctgccg cggcgaaccg tcaccccggc gggcctggac ccgacggagc ggaagcccct
     1081 gccgtccgac ccgttcccgg ccctggatac gggcggggtg gggcagctca tcgagctggc
     1141 gctgcgcaag gggcgcgaga cgcggccgga cctggcgtgc ggcatctgcg gcgaactcgg
     1201 cggcgatccg aacagcgtga agttctgctt caagatcggc ctgaattacg tcagcggctc
     1261 cccgtatcgc gtgcccgtgg cgcggctggc ggccgcccag gcggccatca cgcagtagcc
     1321 gttccatcgc gggcggagac cgcgcttcgg gaacgcttga ttttcccggc cgaaagctgc
     1381 gagaatatat cgcttggaat agtgcggctt aaaccgccgg aaggcgcggc acgatggaag
     1441 gcgaagacag aggcatcaag atctacctcc gcgagatcgg gcagacggcc ctgctgaccc
     1501 gcgaggacga ggtcaagctg gcccgccgca tccgccgggg cgacgaagag gcgcgccagc
     1561 tcatgatccg cgccaacctg cggctcgtgg tcaagatcgc ccacgactac gcccagtacg
     1621 gcctgcccct cctcgacctg atctccgagg gcaacatcgg actgatgaag gccgtcgagc
     1681 gcttcaatcc ccgcaagggc gggaagttga gcacgtacgc cgcctggtgg atcaagcagt
     1741 ccatcaagcg ggcgctcgcc aaccagagca agaccatccg cctgccggcc cacctggtgg
     1801 acaagatcgc ccgcatgcgc cgggcggaga gaaacttcgc gcagaagaag ggacgcgatc
     1861 ccaccgagcc cgagctggcc aaggcggtcg gcgtatcggt cgccaccatc cggatgtgga
     1921 agacggtttc gctgcggccc gcctcgctca acgcgccgat cggcgacgag gacagcgccg
     1981 agtacgggga catcatcggg gacgaccgga acgtctcgcc ggtggaggat ttgtccgacc
     2041 gccagctgaa gggcgagatc gaggggctgc tcgaccggct aagcaagcgc gagcgggaga
     2101 tcctgatgta ccgctacggc ctgcgcggcg tgaaggagga gaccctcgag gttgtcgggc
     2161 ggcgcttcaa gatcacccgc gaacgcgtcc gccagataca gaattccgcc gtcttgcggc
     2221 tgcgccagat gttggaggac atggatcagc cccaggatag cgccgagccc acggagaaat
     2281 acccatgacg atcccggccc tgaaaaacgc catccgcgac atcccggact tccccaagga
     2341 aggcatcatc ttcaaggaca tcacgcccct gctcgccgag ccctcgctct tcaagctggc
     2401 ggtggatacg ctggccgggc ggcaccggga cagccgcatc gaccgcgtct gcgtcgtcga
     2461 gtcgcgcggg tttctgttcg gatccgccgt ggcctaccag ctcggcgccg gcctggtgcc
     2521 gatccgcaag aagggcaagc tgccccacga gacgctggag gcggcctacg acctcgagta
     2581 cggcaccgcg acgctccagg tgcacgtgga cgccttcgag cccgggacgc gcgtgctgct
     2641 gatcgacgac ctgctggcga ccggcgggac ggcggaggcg tccgcccggc tcatcgagca
     2701 gctcggcggc aagctcatcg agatccagtt cctcatcgaa ttggcgttcc tcaacggacg
     2761 cgagaagctc acgaaatatc ctgtcttcgc gccgatcgta tacgattgat gcgcaggccg
     2821 ggtggttcat tttgcgcttt acagaattgg ccaacggatc taatgtaaag cgctttactt
     2881 atcggccaac cgggagttcc gtcatgtatg ccatgaatcg agttgcaatc atctgtcttg
     2941 ccttgagcgt gctggcgcgc gttggccgcg cccagtcgcc tacgatagac ggcgtgctcg
     3001 acgcggattt ctacggcgcg gcgcggtcgg ttcaggacac tccgaccagt ttcggtgacg
     3061 ccacgaacgg acacatccgt ttcgcgcgcg gcggctcgga actcgacgcg gcgtacgccc
     3121 ggatttccga cggcacgctg ttcctgttca tcgccggcaa cgtggagacc gccggacagg
     3181 gcatccagtg gccggtcgga aaccggaaca agctggatat cttcatcgac agcatcgaag
     3241 gcggccagag cagcctccgc ggcgacaatg cggacgtgga cggaaacggc ctgaaccgga
     3301 tgggccacct ggacccgtcg aacgacggcc ttaagttcga cacgggcgtg gcgcacgact
     3361 tcttcctgac attcaacaac tacaccgagg tcgtgttcat ccccggctcc ggcaaccagg
     3421 aactgtggcg gggccagttg tactacgcca gcctgccgac cggtggcagc ggcaccggca
     3481 cgctcgtcgg caccgcgtcc gattcatggc ccggctcgta tcccgattcc ttcacgctgt
     3541 cacagggcgt gcggatgggg ttccgcaaca gcaacaccaa cggcgtcacg ggcacgggcg
     3601 cgccgtcgcc cagcgcggcc gacgccccga acgtgacgtc cggcctcgaa ctggcgatcc
     3661 cgctggcgct gatcgacagc acgaacggcg acctgaacgc cgagatccgt atcaccgcct
     3721 tcatcaacga ctccgctcac ggctacctgt ccaaccaggt cctcggcccg atgggccagc
     3781 cgtccggcag ctacggcaac ctcgccgagc cccgcgtctt cgatttttcg cagtcctact
     3841 cgcccggcgc gcagtatttc accgcggaca acgcctatcc ctcgacgagg ctgatccttc
     3901 cggtaacgct gctcgacacc ggcagcatca gcaaccgctg gatcggcgag gtcggcgcca
     3961 cgtacgtgct gcaatacacg accaacctcc tgaccggccc ctggaccaac gtcagcggcg
     4021 tcgtcacggc cgagtcgcca ctggtctcgt tcgtggacac gaattccgcg gcgtcccgca
     4081 tgtattaccg caccgcccgg ctgccgtagt aaacgagaga atcctccttg acccgccggg
     4141 ggggacgcgt ggttaatccc cggtgacatg gaccgccgcg aactgcccat tttcgagatc
     4201 gagcgcgaac tgctcgcgcg ggccgccgaa caccgccgcc tgatcgtccg cgcgcccacc
     4261 ggctcgggca agtccacgca gattccccag atgctcctcg acgccgggct cctcaagagc
     4321 ggccgggccg tcatcctcca gccccgccgc ctcccgaccc gcctgctcgc cgcccgcgtg
     4381 gccgccgagc gcggggcgaa gctcggcgcg gaagtcggtt accagatccg cttcgagaac
     4441 atcgccggcc gcgacacgcg catcctctac gagaccgagg gcgtcctgct ccgccagatg
     4501 ctttcccatc cccggctcga cggcgtcgcg gccatcctgt tcgatgagtt tcacgagcgg
     4561 cacctgtacg gcgacgtcac actcgcacgc gccctccaac tccagcgcgc ggcgcggccc
     4621 gacctgctgc tcgtcgtcat gtcggccacg ctgccctccg gcccgctcga cgagtacctc
     4681 gcgccctgcg ccgccctgga agcccacggc cgcctgttcc ccgtgatcac ggaatacctc
     4741 gaccgccccg cggacttcga ccggaccccg ccatgggaag ccgccgccgc cgagttcgag
     4801 cgggtatgcc gcgaaggcga accgagcgac acgctggtct tcatgccggg tgcctacgag
     4861 atctcgcgga cggtccaggc cctcgagaac tcgccggccg cgcgcgggcg gatcatcctc
     4921 ccgctgcatg gcgagttgcc taccgccgac caggacgccg ccgtggcgcg ctatgaccgg
     4981 ccgaagatca tcgtcgcgac caacgtcgcg gagagctcgt tgacgatcga gggcgtgcgg
     5041 ctggtcgtgg acagcgggct ggcgcgaatc ccgcggttcg acccggcgcg cgggatcaac
     5101 acgctgctgg tggaaaagat cagccgggcc tccgccgacc agcgcgcggg ccgcgccggg
     5161 cgcacagcgc ccgggcgctg cgtgcgcctg tggacggaac gcgaacacgc ggagcggccc
     5221 gtccaggagg tgcccgagat ccggcggctg gacctgtccg aggtcatgct gaccctgcgc
     5281 gccgccggag cgggcgacct gcgcgaactg ccctggcttg agccgcccgg cgtgagggcc
     5341 ctggaccggg ccgagatgct cctgcgggac ctgggcgcgc tggatgaaaa gcaggccgtg
     5401 accgagctgg gccggcggat ggccgggttc cccgtgcatc cccgttatgc ccggatgatg
     5461 atcgcggccg gggagcacgg gtgcgtccgc gcggtcgccc tgatcgccgc gctgacccag
     5521 ggccgctcgc tctggatgcg taagccggac aagtccacgc tcgagcagcg ggaggacgtc
     5581 ctcggcgagg agggcgcctc ggatttcttc gggctcatgc gggccttccg gttcgccgac
     5641 agccggcgct acgatgtcgg cgcgtgcaac cggctgggca tccacgccaa caccgcccgg
     5701 caggtccggg cgctggccgg gttcttcctc gaccttgccg aaaaaaccgg gctgaagatc
     5761 gaggcccgcg cgccggatac ggacgcgatc tgcaagtgca tcctgacggg cttctccgac
     5821 caggtggcga aacggttgga tgccggaact ctacgctgcc agttggtcca cggccgcaag
     5881 ggcgtgctgg cgcgggaaag cgccgtgcag ggccccctgc tcgtcgtgtc cgaggtccgc
     5941 gaggtagagg ggcgcggcgg cgagttgagc gtgatcttga gcctggcgac cgcgatcagg
     6001 gccgaatggc tcgaggagtt gtttcccggc gatttcagcg agccggtgga cgtcgaatac
     6061 gacgcggccg agcggcgggt cgtcgcctgg cgccggcgga tgttccgcga cctgcccctg
     6121 gagagcaagc gcgtggatcc gccgcccgcg gacgccgccg gggacctgct ggccgaggag
     6181 gtcatcaacg gccgcatcaa gctgccgcac tgggacgaaa ccaccgacca gtggatcgcg
     6241 cgggtcaaca gcctcgccgc ctggtgcccg gaactcggcc tcaccgccta ctccgaggaa
     6301 gaccgctggt cggtactccg cgtcctgtgt cgcggcgcgg tctccgcgcg tgatctcaag
     6361 gaccgcccgg tgacggccgt gttcaaagac cagatggacc cggcccgtcg cgcgctggtg
     6421 gagaaacacg cgcccgagcg gatcgagctg tccaacgggc ggcgcgcccg cctgacctac
     6481 accgagggcg aaccgccgca catcgcccag cgcatccagg atttgtacga cgtgaaagag
     6541 actcccagga ttgcgaaggg ccgcgtgccc gtagtcgtcg aaatcctggg acccaaccac
     6601 cggcccgtgc aggtcacccg cgacctggcc gggttttggc gcgatcatta cccgcgcgtc
     6661 aagaaagaga tgcagcggaa gtatcccaag cacgaatggc gatgaagaac cccttttccg
     6721 aagcctttcg cattgtgccc gatgactggc tgaacccgct gccgctggag cgctggttcc
     6781 ggccgagcgc gccgctggag gtggacgtcg gttgcggcaa gggccggttc ctgctcgcgc
     6841 gcgcggccgc gcatccggac accgacttcc tcggcgtgga ccggatgatc gggcggctgc
     6901 acaagatcga ccgcaagctg gtccggcgcg ggctgtccaa cgcgcgctgg ctgcggctag
     6961 acgcgtacta tgccgtggcc tgcatgatcc cgccggaggc ggtgaccacg tactacattt
     7021 tctttcctga tccctggccg aagaagcggc accacgacca tcgcctgttc aacgcggcct
     7081 ttctcgacgc gctgttccgg acgctgaagc ccggcgggca ggtgcatttt gccacggacc
     7141 atctcccgta cttcgacgag gttgtggccg cgatcaagac cgacccgcgc tttgcggaaa
     7201 tccccgcctt cgagcctgcc gaagacgagc ggactgattt cgagctgata tatttggggc
     7261 agaccccgat cgggcggtat tccttcgaaa agcgtcgcgg gggggctctg tgaatgatca
     7321 gaacgtcatg atgatgtttt ccattggcgc cctcgcgatg acggcccttg cgctcgctta
     7381 tggtgcgcac ggacaccggc acgtacgccc gagatgggtc tgtctggtta tcgccaatct
     7441 gctgcttggt ctatggctag tggccgttgg gttgctcgcg ggcgaagtgt attttcgcca
     7501 ttttctggac atgaccgatt cctttgcgat aagtcgggtc caccaagcat ggttgcatcg
     7561 ccattggacg ttgaataagc aggggattcg cgacaccctt cagagttaca cacttaaacc
     7621 tgcgcccgga aaagaacgca tcgtattcct gggcgattcc ttcacggccg gacatggtat
     7681 ccgcaatgtc gaccaccgct ttgccaacct gtatcgggcg atgcatgctg aaaaggaagt
     7741 ccacgtactg gcacatagcg gctgggatac aggcgatttt ctgaattgcc tgcaggattt
     7801 caaacgacag ggatatgttt tcggcgaggt cgtgctgata tccggcttga atgacatcag
     7861 cgatatagat ccagcttggc agcggatgac ggcgcaggtg aaagattcct tcaagccgtc
     7921 tggtattctc cagaacagtt atttactaaa ttggctctac tttcgcatga aggtggtgac
     7981 gagtccggcg tttcgtgatt atggcctcgg cgttcaggaa cgcgtacgcg ggcccggtgt
     8041 ggaacgaaca gaaaatgagg atgcgtacac tacgcgacat cgtccagtca gccgggggcc
     8101 gattggtggt cgtcacgctt ccctttttca actacccggc cgaaacatat cctttcacgg
     8161 cgattcacga gcaactggat gacttctggc gcggggaagg cgttccccat ctggatttac
     8221 taccgatact aaacagacaa caccccaagg atttagttgt aaatcgattc gatgctcatc
     8281 ccaacgaatc ggcgcacaaa ctgatcgccc gcgagattga cctttttttg accggactgc
     8341 cacggtagat ccggcgagaa caatgggggc gggagtcgat tcacaacaga cgacgcccga
     8401 ccccttgtta caccttattc cacaaacaag gacaccggct ggaatttcgt cacgtccacg
     8461 aggcccttgt cggtgagttt caactccggg atgaccggca gggcgaggaa ggacatggtc
     8521 atgacgggat cgggcagcgt gcagcccagc ccgtgcgcgg ccgcggccat ggcctcgatc
     8581 cggtctcgga cctcctccag cggcagatcg gacatcaggc ccgcgatcgg cagcgggacg
     8641 gaggccagga cctgctcgcc gttgaccacc gcctgcccgc cgcgcaggcg cgcgacttca
     8701 cggatggcgg ccatcatgtc ctggtccgtc gcgccgacca cgacgatgtt gtgcgagtcg
     8761 tgcgcgacgc tggacgcgat cgcgccgctc ttcatgccga agccgcgcac gaagccgatg
     8821 cccacgtggc ccgaggcctg gtgccgctca atgacggcga ttttgagcag gtcgcgcgcg
     8881 ggatcggcca ccacggcgcc gtccgccacg gccgcctcgt cctcgagaag cttcgtgacg
     8941 atctggtcgg gcacgaggcc gatgacccgg atgcggggcc ggtcggcctt gccctcggga
     9001 accgccacgc gcaggtcgag gccgtgccac tgcacgttga cgctgctgcg gagccggtgg
     9061 ggttttgcct tggccctcgg cgccagcagc gcttgattct cggctacttt ctggcccgcc
     9121 tggtagacct gcgccacgcg gaaggattcc aaatcgtcga tcaccacgag gtccgcccaa
     9181 tagccaggcg cgacagcgcc gagtttcttg agcccgaagt agcgcgccgg gtggagcgtg
     9241 cccatgcgga cggcggtgac gggatccatg cccttctgga tggcgcggtg gaccagccag
     9301 ttgatgctac cctcgcggag gaggtcggcc gggtggcggt cgtcggtgca gaagctgcag
     9361 ttgatggcgg cggcgggcgt gaccagcggc agcagggcgt cgaggttgcg cgccgtcgtg
     9421 ccgtcgcgga tgaaaatatg catgcccatc tgtcgcttct cgcgcgcctc ttcgagcgtc
     9481 gtgcactcgt ggtccgagcc gatgccggcg acgatgtagg cggcgaggtc cttgccggac
     9541 aggccggggg cgtggccgtc gatcgggtgt ccctcggcgg cggtcagctt ggccagcacg
     9601 tccggggcgc ggtagatcac gccggggtag ttcatcatct cggccaggcc gatgacccgc
     9661 gggtgctgga acaggagcga gaagtcctcc gcggtcagcc gcgcgccgga ggtctccatg
     9721 tccgttgccg gcacgcagga cgggaccatg acgaacacgc gcaggggcag gccctcgctg
     9781 gtctcgatca tgtagcgcac gccgtcgaga cccaggacgt tggcgatctc gtggggatcg
     9841 gtgaccaccg tcgtcacgcc gcgcggcacg accgcccgcg cgaactcggg gacggtgacc
     9901 atgctgctct cgatgtgcac gtgcgcgtcg atgaatccgg ggcacagcag ccggccttgc
     9961 aggtcgacgg tttcgcgggc ctcgtacttg ccgaagccga tgacgtggcc cttgaagacc
    10021 gccacgtcgg cgggatggat ttcgttggag aagacgttga cgacccgcgc gtttttcagc
    10081 agcaggtcgg cgggggtttc cccgcgagcg gccgcgatca tttcagcgtt catcaggacc
    10141 tccgttttcc cacccgccgg aggtagggcg gcccgggccg ccgcggcgcg ctcggcgagc
    10201 gcgccctacc ggccgacgtc acgcgttgtg ttgctgaagc ccgcgccaga tcttctccgg
    10261 cgtgaacggc gcctcgcgca ggcggacgcc cacggcgttg taaatcgcgt tggcgatggc
    10321 cgggcagggc ccgttgatgc cgatctccga cacgctcttc gcgccgaacg gcccggtctt
    10381 ctcgtacgtc ggcaccagga tcgtcaccat ctccggcttg tcccgcatcg agtagatctt
    10441 gtagtgcagg aacgagtcgt tgagcatcct gcccgcggaa ttgaacaggt actgctcggt
    10501 gagggcgtag cagatgccgt tcatgacggc cccttcgacc tgtccctcgg ccagcttcgg
    10561 atgaatcgcc gtgccgcagt ccgtcgccgc gacgtacttc accacgcgga tcaggcccgt
    10621 ctcggtgtcc acctcgacct ccgcgaagtg cgccgcgaac ggcggcgggg acttgtggct
    10681 gatgtgcgag gagatggccg cgatctggaa ctggttggcc gcgtacaggg agtgcagggc
    10741 gacgtcctgg aacgtcaccg agccgctgcc gtccctggcg aagacctgcg cgtcccggat
    10801 ttccaggtcg gcgggcttct tctccatcat ctccgcggcg acgccgagga tctgcttctt
    10861 gatcttcagg cccgcgtcga gcacggcgtt gccggtcagg taggtcgtgc tcgaggcgta
    10921 ggcgccgacg tcgaacgggg tcatgtccgt gtcggaggag tacacgagga tcttgtcgct
    10981 ggtcgtgccg atggcctcgg cggcgatctg ggcgaggacc gtgtcgctgc ccgtgcccag
    11041 gtccgtcgcg cccatcagca ggttgaacga accgtcctcg ttcatcttga gcgaggccgc
    11101 gcccatatcg atctcgggga tgctcgagcc ctgcatcagg cagcacatgc cgatgccgcg
    11161 gcggtagcgg cccttcttct tgcgccagtc gccgcgcttc ttccagccga tcgccttggc
    11221 gcccagccgg atgcacttgt ccaggccgca gctcccgatc gcctgctcca cgcccgcctt
    11281 gccctcgccg agggcggcga acacggggga ggtctcgccg gagcggatgt ggtggttcga
    11341 ccgcaggacc agcgggtcca cgccgatctg ctcggccagc tcgtccacgg cgctctcgac
    11401 ggcgaaggcc gcctgggtcg cgccgtagcc gcggtacgcg ccgccgacgg gcaggttggt
    11461 gtagaccgcc gtgccggtga agtgaacgtt cggggcgcgg tagagcggca ggaccttgct
    11521 cccgctgttg cagaccacgg tcagggcgtg ggcgccgtac gcgccggtgt tggaaatgca
    11581 gtccagcccg accgccgtca gggtgccctc gggatcggcg gccaggcgaa cgcggatcct
    11641 ctccgggtgc cgcgtgcggc tgctgacgaa ctcctcctcg cgggtgtaca tcagcaacac
    11701 cggccggcgc gatcgccagg ccagcatgcc gacgacgtct tccagcagca cctcctgctt
    11761 cgcgccgaac ccgccgccga tgcgcggctt gatcacgcgc acctgctgga ccgggatatc
    11821 catcgtctgg gccgcgatgc gccgggcgtg gaacggcacc tgggtgctcg tccggatcac
    11881 cagccgcccc tcggcgttca cgtgcgccat cgtcacgtgc ggctcgatgg ggcagtgctg
    11941 ggccatgtgg atgtaatagg agttatccag cttgtacggc gcgccggcca tcgcctgctc
    12001 gacgttgccg acgtccatgc cgacgcgcgc ggcgatgttc ttcttcggct cgtacgggac
    12061 ggggatcacg gcccgggcct cgggctcgtc gtggatcacc gggctgccct ccttcagggc
    12121 ggcctcgatg gacaggaccg gctccagccg ctcgtattcc accttgatcg ccttcacggc
    12181 ggcctcggcg atctcgggcg tctccgcggc caccgcggcg acgcggtcgc cgacgtagcg
    12241 gaccttgcgg tcgaacatga acgtgtcgta gggcgacggc tcggggaagc cctggccggc
    12301 cgtcgtgtgc gcgatgcgcg gcacgttgcc gtgatagagc acgcagtgca cacccggcat
    12361 cttctccgcg gcgctgatgt cgatgctctt gatgatcgcg tgcgcgtgcg ggctccagag
    12421 gagcttcgcg tacagcgcgt ccgccggctt gaagtccgcc acgaacagag gcttgccgag
    12481 cgccaacggc agggcatcga gcttcttcac cggctggttg actaccttga agttcttttt
    12541 cgccatggtc gttctcccgg atgccgtcag gcctgcgccg gcttgttcgc gtcgcgcacg
    12601 ttccgcagcg ccgtccagat cttctcgtag cccgtgcagc ggcagaggtt cccgtccgtg
    12661 tggacccgga tgtcctcgtc gctcgcggcg gggttctcct cgaacatcgc cttggcggac
    12721 atgacgaggc cgggcgtgca gtagccgcac tggaccgcgc ccgcgtcgac cagcgcctgc
    12781 tggatcgggt ggacccggtc gaagtcgccg acgccctcga tggtcacgac gttccggccg
    12841 tgggccagga aggccggcat gatgcaggac agcaccgcgc ggccgtccac gttgaccgtg
    12901 cacgcgccgc aggtgccctc gtcgcagcct tccttggtgc cggtcaggcc cgagcggcgc
    12961 agcagcttca ccagcagctc gccgggggcg atctggaaat cccgcttctt tccgttcagc
    13021 gtgaaggtga tggtcctggt ggtgctcatg atgcctgctc cttcgcctgc gcccaggcct
    13081 cgaccagggc gtcgcccagc gtaacggccg ccagccgccc gatgtattcg gcggacatgc
    13141 cctcggcctc cttgaatttc aggcccgaca gcccgttcag cgcggcctcg cgcagcagcc
    13201 cctcggaggc cttcttcccg gcgagcgcgg cctccacggc ggcacagcgg gccgggaacg
    13261 gcaccgcggc gctcaacgcc gcgcgggcgg agcgtatgcc cccgccgtcc aattgcaggc
    13321 aggccgccgc cgtcgccagg ctgaagcccg ttgccgtctg ccgcaccttc ttgtagccga
    13381 agcccgcgcc cgcgccgacg gcgggcaccc ggatatgcgt gagcaggtcg ccgccccgga
    13441 acaggcgggc gggttgggat gagaagaact cgtcggcggt catttccttc tcctgcgcgc
    13501 cccggatcac cagcccggcg cccagcgcca gcagggccgc cgggaaatcg gaccagggga
    13561 acacgcggca gatattgccg ccgagcgtcg attcgttgcg aatctgctgg gaggagagcg
    13621 tcagggccac gcggtcgagg acccagccgg gctcatggcg cttctggagc gccgccacgc
    13681 gggtggccgc gccgatgcgg aaggcgtccc cgtcccggcg gatgccgtcc aggccgagcc
    13741 ggttcaggtc caccgcggtc atcggctgat cctcgggaat gaagaccagc gaggtggcgc
    13801 cggccacggg gagcgccgcg gggccgagat cccggagcgc ggccagggct tcgtccaggc
    13861 gcgtgggtat cttgaaatcg gtaatcttca cgctgtgtcc tccgagtgtc tgttccgtca
    13921 atcgaggccg gtgatgggcc ggcggcgcct atgaatccag catcgtctta taccccagcc
    13981 ggttagcccc tgtcatcaga aaagaccgcc cggatcaggg cgcgcgcgcg cggggcgcgt
    14041 cgtggggcgg ttgcttcgcg ccggggagcc gcgcctccac gaggcgggcg cgctcgatgc
    14101 gccgggcgat ggagggatgg ctgtacagca ggaactccag ccaggccgcg gggcgccgca
    14161 cggccaggtt ctgcgcggcc agcttgttga gcgaggaaac gaggggggcc gccgagcccg
    14221 tcgaccgcac ggcgtactcg tccgcggcga gctcccggcg ccgggagtag gcgttggaga
    14281 agggcatcgc gacgagggag aacaggaaga ggcagaagac caggaggggg aacgagccga
    14341 tgtcgtccgg gccgccgaag cggaaggcga cgaccagccc cttgagcgcg gcctgggcga
    14401 tgaagaagcc gagggcggcc aggaccgtgc ctgccgtgat caggcgcgcg atgtcgctgt
    14461 gccggtaatg ccccagctcg tgcgcgagga tggccaggat ttcgtcccgg ccgtatccct
    14521 tcaacagcgt gtcgcccagc aggatgcgac gggtccggcc cagaccggcc agcgcggcgt
    14581 tggcggcctc ggtcttttcc tccagtcccc agcggaacac cccgaccacc cggacgcccg
    14641 actgccgggc caggtcctcg gccgccgcca cgatctccgg gctctcgagc ggctcgatct
    14701 tgtggaacag cggcaggat
//
LOCUS       DAOOTK010000084        14612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2792_length_14612_cov_4.509377, whole genome shotgun sequence.
ACCESSION   DAOOTK010000084 DAOOTK010000000
VERSION     DAOOTK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_14612_cov_4.509377"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1493)
                     /locus_tag="PK634_09720"
     CDS             complement(90..1493)
                     /locus_tag="PK634_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98054.1"
                     /translation="MRRVLSSLWMAGVLLLAAAGGCRTVPPPVVIVNPEAPDLAALSA
                     EGQARADSLACYAKGLLDERRRDYASALSNFQAAARLAPDNEELALRVALLLLQQRRV
                     DEAVGVMDEATRHQPDSDKILRWTILVHRAAGHTERVIELYRDLIRREPANADPYIEL
                     TALLIQEGRHDEAIRLLESGRDRVDEPLDLLRLLAGLHAEKAEAATDDREAGRSRAAA
                     IQILESAREKAPADTSILFQLGDLYIADQQIERAVEYFEAVEQEKPDSLLIRQKLALS
                     FLKLGDKDRAIQALEELTRQAPGHPRLFFYLGELYHDKGDTEKALFNLSLAEKAFPDD
                     PTPLLRKALILSEDLKQPAEAARALEEGLENRPEDVHLLEMLAYVLYDMKDYARALAL
                     FEKRHELAVRDEDGASPLFYFNYALAAEAADRVDEAAKHLHEAIQLNPAYIEAYLRHA
                     FDQEDDASLSRSKAWRR"
     gene            complement(1600..1689)
                     /locus_tag="PK634_09725"
     CDS             complement(1600..1689)
                     /locus_tag="PK634_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOW98055.1"
                     /translation="MGSVKKKRRKKMAKHKRRKRLRAMRHKKG"
     gene            complement(1724..2608)
                     /gene="hisG"
                     /locus_tag="PK634_09730"
     CDS             complement(1724..2608)
                     /gene="hisG"
                     /locus_tag="PK634_09730"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOW98056.1"
                     /translation="MTETTRKLVLGLPKGSLQEATFLMMKKAGFNVSVGSRSYLPTIN
                     DPELEGRLIRAQEISRYVEHGMLDAGITGYDWILENESDVHVVADLIYAKQGLRPVRW
                     VLAAPEKSRIQSVKDLQGKRIATEAVSLTKQYLRRHKVKAEVEFSWGATEAKAPELVD
                     AIVELTETGSSLRAANLRIVDEVLTSTTKLIANRKAWKNPWKRAKIEQLALLLSGALA
                     AETKVLLKLNVNEKNLKKVLAILPALHAPTINSLSSTGWFSVESVVEEPVVREIIPAL
                     KRAGAEGIIEIGLNKVVP"
     gene            complement(2743..3393)
                     /gene="nth"
                     /locus_tag="PK634_09735"
     CDS             complement(2743..3393)
                     /gene="nth"
                     /locus_tag="PK634_09735"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOW98057.1"
                     /translation="MTRRETDAVFRRLTRAYGPPPRLRRLRPLDALVQTILSQNTSDT
                     NSWRAYQELTRTFRSWPAVAAAPARSIERAIRSGGLARTKAPRIRLVLRLIREREGRL
                     SLARLKRLSDPEARVWLTSLPGVGSKTAACVLLFALGRPVMPVDTHVERVCRRLGWVG
                     AKLPVDQIGPVLERHVPARRMLAMHLQLVRHGRRTCRARKPACARCPLRSCCRNAQ"
     gene            complement(3390..3998)
                     /gene="purN"
                     /locus_tag="PK634_09740"
     CDS             complement(3390..3998)
                     /gene="purN"
                     /locus_tag="PK634_09740"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOW98058.1"
                     /translation="MLSLGILGSGRGSNARSILEYIEAGRLDARAACVVSDVAGSGIL
                     ELGRMHGIPSFHVDGAPFRTKLDGAAQDQVIGLLREHGVDTVALAGFMRMVKPALLRA
                     FPGRVVNIHPALLPAFPGLEAWEQALAAGASESGCTVHFVDEGMDTGPVILQRRVPVL
                     PDDTPASLHARIQEQEHLAYPEALALLASGRVRLEGGKVVRS"
     gene            4258..4575
                     /locus_tag="PK634_09745"
     CDS             4258..4575
                     /locus_tag="PK634_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOW98059.1"
                     /translation="MGTRLYVGNLSFNTTEGELQRLFEQAGKVTSCNIIMDKFTNKSR
                     GFAFVEMSSQDEANKAIATLNGKEVDGRALTVNEARPREERPRGDFGGGGGREWRGGD
                     RRR"
     gene            4711..6108
                     /locus_tag="PK634_09750"
     CDS             4711..6108
                     /locus_tag="PK634_09750"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOW98060.1"
                     /translation="MQQRIHSIARWIGLATVLAGIPVRGGEALPLPAVEWKAGDRVLV
                     LAPHPGDETIGCGATIRQLTATGIPVRIVFLTCGEHSQWSLQLYRERLVVNPDKVSLL
                     GPRRMQEARAAAGELGLAADSLLFLGYPDFGTLNIWCAHWAEAMPFRSLLTRTREVPY
                     ADALHPGAPYKGEVLLADLKDVIRDFRPTCILVSHPADQHPDHQALYLFSRVALWDLS
                     DEPEPVRRPYLIHYTHWPDRDGTSWLLPPTDLAGAIDWHAVAADPEQAASRDRALAAH
                     QTDETFSSGRLRLFARANDLYGDFADADLSVGPETLESGKAPQILPEELTAEEKERHV
                     GVEACTMSLEGRIVQVDLRLNRALEPGMGASVYVFGYRPDHPFEHMPKLIITLKAFSA
                     EVLDQARPCPPGLVSVKRSGADIRLRIPLEALGSPDRLLAGARTYLNDVPLDWVAWRV
                     LRVPSGADSSASVEP"
     gene            6438..6527
                     /locus_tag="PK634_09755"
     tRNA            6438..6527
                     /locus_tag="PK634_09755"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6472..6474,aa:Ser,seq:tga)
     gene            6538..6783
                     /locus_tag="PK634_09760"
     CDS             6538..6783
                     /locus_tag="PK634_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOW98061.1"
                     /translation="MYFVYVLKSQTGSRLYIGSSASPEERLAAHNAGRVRSTKAYRPW
                     ARILLETHPDRTTAEKRERYLKGGWGHRWLKQAGLAT"
     gene            7034..7621
                     /locus_tag="PK634_09765"
     CDS             7034..7621
                     /locus_tag="PK634_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOW98062.1"
                     /translation="MESDAEIVRQVLDGDANAYERLLRRHAPRGFEAVGRRVPAADVP
                     AVAQDVFLAAYRSLGAYGQRNPFDHWLLRIARRRCCDYWRARGRREGRFAESLDADER
                     AELDRVMAATAREQQRESEAREESISLVQKALASLDAEDRAMMEAVYFEEAPLKDVAA
                     GFGWSLAKTKMRALRARRALRGILEQWRRGGKESA"
     gene            7618..7920
                     /locus_tag="PK634_09770"
     CDS             7618..7920
                     /locus_tag="PK634_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98063.1"
                     /translation="MKLEFHEQSMAEAWRRTPVPGPTADWMRGVMQAVRREGAPRVVP
                     FPERASAFTWRMAWAAAAAVAVLSFILLARAPSEPQWAWQIMESGAGVTWLASNGD"
     gene            7925..8338
                     /locus_tag="PK634_09775"
     CDS             7925..8338
                     /locus_tag="PK634_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98064.1"
                     /translation="MKTKFWIALLLLFASGLALGFFGGRAWEQARFHAILRAGPPRIE
                     DWVMNKLEEQLKLTDEQLPVIREIVQRTAAEIHRIRDEGRQAFHACWEKALVEIRPGL
                     TEDQRARLDAMDADDLRPRPFKDAPPPEPPPENKP"
     gene            8335..9606
                     /locus_tag="PK634_09780"
     CDS             8335..9606
                     /locus_tag="PK634_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW98065.1"
                     /translation="MKNGWTCLAWLALAASAAAEPMTWEACVRRAAALNPTLVAAREQ
                     FSAAEFNVGSAKSASRPQLSLGGSAEHAEQDGVASGGDASMSVSASAEQTLYTGGRNA
                     ASVDAARAALDSARADYERADAEVTYNLRRAFVNLLLAQEQVEVLRQIEQRRRDNLEL
                     VNLRYEGGREHKGSLALIQASLNQAEIQVRQGQREVEAARRILLRTMGEERADDTLTV
                     SGSLTCRPAPDSVDWQAEAEGTPAYAQALASTRSAEAGVRSARSGYRPDISLSATAGR
                     SGNEDVFDTDRWAAGVRLSFPLWSGGRTRYEVASARASLARAEAQESETVNGLINDLN
                     ESWVSYCNAVENVVVQESYFAAADLRAEIAREQYGAGLLTFENWDVIENDRISYQRQL
                     LQSQQQALLAEAAWRYVTGRGDFPAENEVSR"
     gene            9603..10562
                     /locus_tag="PK634_09785"
     CDS             9603..10562
                     /locus_tag="PK634_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW98066.1"
                     /translation="MKKLLLFLVLAAAAGVGWYGWTHREKAAVKAPSFEPVEFTRERI
                     EVAIEATGVVEPGNRIEIKPPIGGRVEEVLVVEGQAVRQGEVIARMSSTDRAALLDAA
                     RAQGEAVLRKWEEVYKATPLIAPLDGTIIARKAEPGQTLTAADTALVLSDRLIVRAQV
                     DETDIGLVRGGQEATLTLDAYADDRFRGQVRHIAYEATTVNNVTIYEVEVEPATVPER
                     MKSGMTATVKFVVEAADDALTLPLDALVRRDGRSVVLLAPRGPGARPEEREVKTGLTG
                     GGRVQILEGLDGSERVVRSSFDLPKRQESGSSPFMPRPPGRRP"
     gene            10559..12526
                     /locus_tag="PK634_09790"
     CDS             10559..12526
                     /locus_tag="PK634_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW98067.1"
                     /translation="MIDVRDIHKTYRMGAVDVRALRGVSLSVAPGEFVALMGPSGSGK
                     STLMHILGLLDVPDKGVYRLNGQDVSSLSDRELADVRARSIGFVFQQYNLLPRVSAKE
                     NVALPLIYSDQPNPASPKELLERVGLGDRMDHAPNKLSGGQQQRVAIARALVNRPPIL
                     MADEPTGNLDSRSAVEILGILRDLHASGLTVMIVTHDPEVAARAERVITIRDGRIVGD
                     APTAGKTHHALPAGPDLAGASAGKGRKGWARRLVEGFSMMRQAFRALAANKTRAFLSM
                     LGVLIGTGAVIAVLALGTGAKKSVEERIGAMGANLLVLRPGRIEARGVAMEVGAASRL
                     SLADVDAVRTEVAGVKTVAPTVGGGAQATFGGKNWRTQIVGTVPAYASMRNLTVAVGR
                     FLEEEDARTRARVAVIGATVSRELFGGVNPVGQSIRVNRVAFEVIGVLTESGTGFRFD
                     PNDQIILPVTTAMRRMLGRDYVDTIEIQSTDGDVLSAVETNSVELMRQRHRISPEEEE
                     PFRVFNMADIQKTILDTSRTLSLLLAGIGAISLLVGGIGIMNIMLVSVTERTREIGIR
                     KAIGARRSDILVQFLVEAVVISVCGGVLGILTGWSISVAMSRLAGWAVSVTAPTVLLA
                     TVFSALVGVVFGLWPARKASGLNPIEALRYE"
     gene            complement(12684..>14612)
                     /locus_tag="PK634_09795"
     CDS             complement(12684..>14612)
                     /locus_tag="PK634_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98068.1"
                     /translation="GTTPPRWIGQGIVPPAEWRERWLDDLARFRRAGSLLLSTGARRA
                     GAALIFLFMLLALTRTLLLPPLGWDTLTYHGVKAALWAQQGRPDLFPAPGGWGSKILL
                     PGGSELLQAWALLPFRSDLLLGFLDFVFWLALGAFIVQFARELGLTTKAGALGALYLL
                     FLPALRLQIGSGYGELPLMCALLGGLSYSIHFLRQRDPPSLVIAFAAFGMAAGIKAHV
                     WPALAWAGLVLLAGACCRGRASARKCRPALLAGILAALLMVLPWPALNLRAGRLPLSP
                     FPIRVAGLVLGESTPSYHTYHHREGVRHGDLREEIQAGRDLFATPLSTWPHLGLLTLP
                     LGVAGWISLVRFRRMGAANALLLAGFIGLTAWVFWGPSFAVIRLLWSTTSARFLLPAF
                     VPASLLGLAWLWTKPRLRLVLAAALILLALLHAGRAACQGWAPFEFRVIPKSAGLLAA
                     LAASLVILSRINRRAVVSGVVTAALLAASLTAIQKERDHLRYKALKQSIVLHWVENPW
                     ADGARALDDPAVPRRLAVTTGIDYNGDNWFLYFFLGRRFQNTVTYISPTASGRVLDAQ
                     SVEELRDRVDSRAWLRRLGQAGITHVVSLAPESLELAFMTKHPGLFTPVAGDGGTWGV
                     FEVATSPSSYGRSTPSRK"
BASE COUNT         2421 a         4745 c         5107 g         2339 t
ORIGIN      
        1 ctcgggatag gctttccggg agctgtggag gagggccatg tagtagtggg tggtgggatc
       61 ccccagctga accccgccta tatcgaggcc tacctgcgcc acgccttcga ccgggacagc
      121 gacgcgtcgt cctcctggtc gaaggcgtgg cgcaggtagg cctcgatata ggcggggttc
      181 agctggatgg cctcgtgcag atgcttcgcc gcttcgtcga cgcgatcggc cgcctccgcg
      241 gccagggcat aattgaagta gaagagcggg ctggccccgt cctcgtcccg gaccgccagt
      301 tcgtggcgtt tctcgaaaag cgccagggcc cgggcatagt ccttcatgtc atagagcacg
      361 tatgcgagca tctccagcag atggacgtct tcgggcctgt tttccaatcc ctcctcgagc
      421 gcccgggccg cctccgccgg ctgcttgagg tcctcggaaa ggatcagggc ctttcgaagg
      481 agcggcgtgg gatcgtccgg gaaagccttc tcggccaggc tgagattgaa cagcgctttc
      541 tcggtgtcgc ccttgtcgtg gtagagctcg cccaggtaga agaacaggcg cgggtggccg
      601 ggcgcctgcc gggtgagctc ttccagcgcc tggatcgccc ggtccttgtc accgagcttg
      661 aggaagctca aggccagctt ctgccggatg agcaggctgt cgggtttctc ctgctcgacg
      721 gcttcgaagt actcaacggc cctctcgatc tgctggtccg caatgtagag gtcgcccagt
      781 tggaacagga tggaggtgtc cgccggggct ttctcgcggg cgctttcgag gatctggatg
      841 gccgccgccc tggagcggcc cgcctcgcgg tcatcggtgg cggcctcggc cttctcggcg
      901 tggaggccgg ccagcaggcg gagcaggtcg agcggttcgt cgacccggtc ccgcccggac
      961 tccagcaggc gtatggcctc gtcgtgccgg ccttcctgga tgagcagggc cgtcagctcg
     1021 atatagggat cggcgttggc cggctcccgc cggatcagat cgcggtagag ctcgatgacg
     1081 cgctcggtgt ggccggcggc gcggtgaacc aggatcgtcc accgcaggat cttgtcggaa
     1141 tccggctggt gccgggttgc ctcgtccatt accccgacag cttcgtccac ccggcgttgc
     1201 tggaggagga gcagggcgac gcgcagcgcg agttcctcgt tgtccggggc caggcgcgcc
     1261 gccgcctgga aattgctcaa ggcggacgcg tagtcgcgcc gccgctcgtc cagcaggcct
     1321 ttcgcgtagc aggccagcga gtccgccctg gcttggccct cggcgctcaa cgccgccaag
     1381 tcaggggctt cgggattgac gatgacgacc ggcgggggaa ccgtccggca tccgccggct
     1441 gcggccaaca gcagcacccc ggccatccac aggctggaca acacacgccg cattcccgat
     1501 ctcccatcct gcgggcccgg tgatggggcc ccttgcgacc gggagccaaa cagcgccccg
     1561 acaggacgtc ggggcgccgt ttcgccatcc cggaaacgat caacccttct tgtgccgcat
     1621 cgcgcgcaag cgtttccggc gtttatgctt tgccatcttc ttgcggcgtt tcttcttcac
     1681 cgagcccatg tgttctcctt cggcgccccg tcggtcggcc ggatcagggc accaccttgt
     1741 tcaacccgat ctcgataata ccttcggccc cggcccgttt caatgccggg atgatctcgc
     1801 gcaccaccgg ttcctcgacg acggactcga cggagaacca gccggtggag ctcaagctgt
     1861 tgatcgtcgg cgcgtgcagg gccgggagga tggccagcac ctttttcagg ttcttctcgt
     1921 tcacgttgag ctttagcagc accttggttt ccgcggcgag ggcgccggag agcagcagcg
     1981 cgagttgctc gatcttggcc cgtttccagg ggttcttcca ggccttgcgg ttggcgatca
     2041 gtttcgtggt ggaggtcagc acctcgtcga cgatacgcag gttggcggcg cgcagcgagg
     2101 agcccgtctc ggtcagttcc acgatggcgt ccaccagttc gggggccttg gcttccgtgg
     2161 cgccccagct gaactcgacc tccgccttga ccttgtggcg gcggaggtat tgcttcgtca
     2221 gcgagacggc ctcggtggcg atgcgcttgc cctggaggtc cttgaccgac tggatgcggg
     2281 acttctccgg tgccgcgagg acccagcgga cagggcgcag gccctgcttg gcgtaaatga
     2341 ggtcggcgac gacgtgcaca tccgactcgt tctccaggat ccagtcgtag cctgtgatgc
     2401 cggcgtccag catgccatgt tcgacgtagc ggctgatttc ctgggcgcgg atgagccgtc
     2461 cctcgagttc cgggtcgttg atggtcggca ggtaggacct cgagccgacg gagacattaa
     2521 agccggcctt cttcatcatg aggaaagtgg cttcttgcag gctgcctttc ggcaacccga
     2581 gcacgagctt ccgggtcgtt tccgtcatcg tctttctccc tatcggttga gcagggcgac
     2641 atcataggcg aggacggggg gcgcgtccaa ggagttttgc gccgatttct cgccgttcga
     2701 tccgcctcct cccacaccct gtgacatgtt ttataaaaca tgtcactgtg cgttccggca
     2761 acaggaacga agtgggcagc gggcgcacgc gggcttgcgg gcgcgacagg tgcgacggcc
     2821 atgccgaacc aactggaggt gcatggccag catccgccgg gccggcacgt gcctctccag
     2881 gacgggtccg atctgatcca cggggagttt ggcgccgacc cagccgagtc gccggcacac
     2941 acgctcgaca tgcgtgtcca ccggcatgac cggccggccg agggcaaaca gcagcacgca
     3001 ggctgcggtc ttcgatccga caccgggcaa gctggtcagc cagacccgcg cctccgggtc
     3061 ggacaaccgt ttcagccggg cgaggctcaa tcgtccctcg cgctcccgta tcaggcggag
     3121 cacgagccgg atgcgcgggg ccttcgtgcg cgccagcccg ccgctccgta tggcgcgctc
     3181 gatggaacgc gccggggccg cagccacggc gggccagctt cgaaatgtcc gtgtcagttc
     3241 ctggtacgcg cgccaggagt tcgtatcgga ggtgttttgc gaaaggatgg tctggaccag
     3301 cgcgtccagc gggcgcaacc ggcggagccg gggcgggggc ccgtacgccc tggtcaggcg
     3361 gcggaacacc gcgtccgtct cgcgacgggt catgaacgga ccacctttcc cccttcgagt
     3421 cgcacccggc cgctggccag cagcgcgagc gcctcgggat aggctagatg ctcttgctcc
     3481 tggatccgag cgtgcaggct ggcgggcgtg tcgtccggca ggacggggac cctgcgctgc
     3541 aggatgacgg ggcccgtgtc catgccctcg tccacgaaat ggactgtgca accggactcg
     3601 gacgctccgg cggccagagc ctgttcccat gcctccagac ccgggaaggc cggaagcagg
     3661 gcgggatgta tgttcacgac gcggcccggg aaggctcgca ggagcgccgg tttgaccatg
     3721 cgcatgaagc cggccagggc caccgtgtcg actccatgct cacgcaggag gccgatgacc
     3781 tgatcctgcg cggcgccgtc cagcttggtc cggaacgggg cgccgtccac atggaaagag
     3841 gggatgccat gcatccgtcc cagttccagg atgccgctcc cggcgacgtc ggaaacaacg
     3901 caggccgcgc gcgcatcgag gcggcctgcc tcaatgtact cgaggatgct gcgggcattg
     3961 ctgcctcttc ccgatcccag tatgccgagc gagagcatgt tcttctccgc ggcccagcat
     4021 agcgggttgc ggcgggaggg caagcgcgcc gggcctgcgc ccgtgacccg cgcaaaaact
     4081 taaaaaaatg agttgtgttt gcgccggggc cggttacatt accgtcgcag gcaggagagc
     4141 aggcagaaag cagccggatg gtcagcaaac cttctttctg acagcatatc agaagcccga
     4201 acgtgtaggg tggtgctgtt ggatactccc tccgaacgat aaaaggaaag caaacagatg
     4261 ggtacaaggt tatacgtggg caacctctcg ttcaacacga ccgaaggaga actccagcgg
     4321 ttgttcgaac aggcaggcaa ggtgacgagc tgcaacatca tcatggacaa gttcaccaac
     4381 aagtccagag ggtttgcctt cgttgagatg agttcgcagg acgaggccaa caaggctatt
     4441 gccacgttga acggcaagga agtcgatggc cgggccctga cggtcaacga agcgcgtccg
     4501 cgtgaagagc gcccccgtgg cgacttcggc ggcggcggcg gccgtgagtg gaggggtggg
     4561 gaccggcggc gttaatcgct ggctgcacca tagagaccga aaaagggcat ccctcgggat
     4621 gccctttttc ttgatccatc ccgctatttt acgtggttgc atccggttgc ggcacgggcg
     4681 agaatggata aacgaaggaa ccgggctctc ttgcagcaac gtattcacag catcgcccga
     4741 tggatcggct tggccaccgt cctggcgggc attcccgtcc gcggcggcga ggcgctgccc
     4801 ttgcccgccg tggaatggaa agccggggat cgcgtgctgg tgctggcccc gcatcctggc
     4861 gatgaaacca tcgggtgcgg agcgaccatc cggcagctga cggcaaccgg catccccgtc
     4921 cgcattgtgt tcctcacgtg tggcgagcac agtcagtggt ccctgcaact ctatcgcgag
     4981 aggctggtgg tcaacccgga caaggtcagc ctgcttggtc cccggcgcat gcaggaggcg
     5041 cgggctgcgg ccggggaact ggggctcgcg gccgacagcc tgctgtttct gggctatccc
     5101 gattttggga cgctgaacat ctggtgcgcc cactgggccg aggcgatgcc cttccggagc
     5161 ctgttgaccc ggacccgcga ggtcccctat gccgacgcgc ttcaccccgg cgcgccctac
     5221 aagggggaag ttcttctggc cgacctgaag gacgtgatcc gggatttccg gcccacctgc
     5281 atccttgttt cgcatccggc ggaccagcat ccggatcacc aggccctcta cctctttagc
     5341 cgcgtggcct tgtgggacct gtcggacgag cccgaaccgg tccgccggcc gtacctcatc
     5401 cattacacgc actggcccga cagggatgga acctcctggc tcttgccccc gaccgacctc
     5461 gcgggcgcga tcgactggca tgccgtggcc gcggatccgg agcaggccgc gtcccgcgac
     5521 cgcgccctgg ccgcccacca gaccgacgag acgttcagtt ccgggcggct gcggctgttt
     5581 gcccgggcca acgacctgta cggcgatttt gccgacgcgg atctgtcggt cgggcccgag
     5641 acgttggagt ccggaaaagc gccgcagatc ctgccggagg aactcaccgc ggaggagaag
     5701 gagcgccatg tcggagtcga ggcctgcacg atgtctctgg aggggcgcat cgtgcaggtg
     5761 gaccttcggc tcaaccgcgc cctcgagcct ggcatgggcg cgtccgtgta tgtgttcgga
     5821 tatcgcccgg atcatccatt cgagcatatg cccaagctga ttatcaccct caaggcgttc
     5881 tccgcggagg tgctggatca ggcgcgcccc tgtccaccgg gcctggtctc cgtgaagcgc
     5941 tcgggagcgg acatccgcct tcgtattccc ctcgaggcgc tcggctctcc ggatcgtctt
     6001 cttgccggcg cgagaaccta tctcaacgac gtgcccctgg attgggtggc gtggcgggtg
     6061 ctgcgcgtgc cgtccggcgc ggattcttca gcctctgtgg agccgtaaac tggcgttgcg
     6121 cgaccttggg gtgtatcgta aaaaaggcca ctgggcttcc cccgtacggg aaaacccggg
     6181 atggacagtg gagggatggc agagcggttt aatgcaccgg tcttgccacg tgaggtcccg
     6241 cgcggcggga ccaacgtggc cccgtatggg gccgcatagc ggccccatca cggggaaaac
     6301 cggacggaca aatggaggga tggcagagcg gtttaatgca ccggtcttgc cacgtgaggt
     6361 cccgcgcggc gggaccaacg tggccccgta tggggccgca tagcggcccc atcacgggga
     6421 aaaccggacg gacaaatgga gggatggcag agcggtttaa tgcaccggtc ttgaaaaccg
     6481 gagtgtcctt atgggcaccg tgagttcgaa tctcactccc tccgccatga tgtgttcatg
     6541 tactttgttt atgttctgaa gagtcagaca ggctcacgct tgtatattgg aagcagcgct
     6601 tcgccggaag agcggttggc cgcgcacaat gcgggacggg tcaggtccac caaggcgtac
     6661 cggccgtggg cccgcatcct cctcgaaacg catcctgata gaacgaccgc cgagaagagg
     6721 gagcggtatc tgaaaggtgg gtggggacat cggtggctga agcaagccgg tcttgccacg
     6781 tgaggtcccg cgcggcggga ccaacgtggc cccgtatggg gccgcatggc agccccatca
     6841 cggggaaaac cggagtgtca ttacgggcgc cgtgagttcg aatctcactc cctccgccat
     6901 gccggctcct ctaacgcgga ttcggtcggc tccccgccgc ccttcgtttc ttgtgacctt
     6961 tcgatctccg ccggcgacgt atatagtgaa cccgggaacg cgttcgggcg aacgcgggca
     7021 cgggaacttc ggaatggaat cggatgccga gatcgtccgc caggtgctgg atggcgacgc
     7081 caatgcgtat gaaagactgc ttcgacgcca tgccccgcgc ggcttcgagg ccgtcgggcg
     7141 ccgggtgccc gccgcggatg tccccgccgt ggcgcaggac gtgttcctcg cggcctaccg
     7201 gtcgctgggc gcgtacgggc agcgcaaccc gttcgatcac tggcttctgc ggattgcccg
     7261 gcggcgctgc tgcgattact ggcgggcgcg gggccggcgg gaaggccggt ttgcggagtc
     7321 gctggacgcc gacgaacgcg ccgaactcga tcgtgtcatg gcggcgactg cccgggaaca
     7381 gcagcgcgaa tcggaggccc gggaggaatc cattagtctt gttcaaaagg ccctggcctc
     7441 cctcgatgcg gaggaccggg cgatgatgga agccgtgtac ttcgaagagg cgccgctgaa
     7501 ggacgtggcc gccgggttcg gctggtcact ggcgaaaacg aagatgcgcg ccctgcgggc
     7561 ccgccgcgcg ttgcgcggta ttcttgaaca atggcggagg ggcgggaagg aatcggcatg
     7621 aaactcgaat tccacgagca atcgatggcc gaggcgtggc ggcggacgcc tgtgcccggg
     7681 ccgacggcgg actggatgcg cggggtcatg caggcggtgc gccgcgaagg cgcgccccgc
     7741 gtggttccgt tcccggagcg cgcttcggcg tttacgtggc ggatggcgtg ggccgccgcc
     7801 gcggccgtgg ccgtgttgag tttcatcctg ttggcccgcg ccccgtccga gccgcaatgg
     7861 gcgtggcaaa tcatggagtc gggcgccggg gtcacctggc tcgcctcgaa cggagattga
     7921 cgcgatgaaa acgaaattct ggatcgccct gctgctgctg ttcgcctccg gcctggccct
     7981 gggctttttt gggggccgag cctgggaaca ggcccgcttc cacgccatcc tgcgcgccgg
     8041 gccgccacgg atcgaggact gggtgatgaa caagctcgag gaacagctga aattgaccga
     8101 cgaacagctc cccgtgatcc gggagatcgt gcagcgcacc gccgcggaga ttcatcggat
     8161 acgcgacgag ggacgccagg cttttcatgc ctgctgggaa aaagcgctgg ttgaaatccg
     8221 gcccggcctg acggaagatc agcgggcccg tctcgatgcc atggatgccg acgacctgcg
     8281 gccgcgcccc ttcaaggacg ctccgcctcc cgaaccgccc ccggagaaca agccgtgaaa
     8341 aacgggtgga cttgtctggc gtggctggcc cttgcggcga gcgcggccgc ggaaccgatg
     8401 acgtgggagg cctgcgtccg ccgggcggcg gcgctgaatc ccacgctggt ggcggcgcgg
     8461 gagcagttca gcgcggcgga attcaacgtg ggttcggcga agtccgcctc gcggccgcag
     8521 ctttccctgg gcggcagcgc ggagcacgcc gagcaggacg gcgtggcgtc cggcggcgac
     8581 gcctccatga gcgtttccgc cagcgccgag cagacgctct acaccggcgg ccgcaatgcc
     8641 gcgtccgtgg acgcggcccg ggcggcgctc gactcggccc gcgccgacta tgaacgggcc
     8701 gacgccgagg tgacctacaa tctccgccgg gccttcgtga acctgctgct ggcccaggag
     8761 caggtcgagg tcctgcgcca gatcgagcag cgccgccggg acaacctcga actggtcaac
     8821 ctccgctacg agggcgggcg cgaacacaag ggctcgctgg cgctgatcca ggcctcgctc
     8881 aaccaggccg aaatccaggt ccgccagggg cagcgggagg tggaggccgc gcggcgcatc
     8941 ctcctgcgca ccatgggcga ggaacgcgcg gacgatacgc tgaccgtttc cggttcgctg
     9001 acctgccgcc cggccccgga ttccgtggac tggcaggcgg aggcagaagg gaccccggcc
     9061 tacgcgcagg cgttggcttc cacccggtcc gccgaggccg gcgtccgcag cgcccgcagt
     9121 ggttaccggc cggacatcag cctctcggcc accgccggcc gatcggggaa cgaggatgtc
     9181 ttcgacaccg accgctgggc ggcgggcgtg cggctgtcgt ttcccctctg gtcgggcggc
     9241 cggacccgct acgaggtcgc ctcggcccgg gcctcgctgg cccgcgcgga agcgcaggag
     9301 agcgagaccg tcaacgggct gatcaacgat ctgaacgaat cctgggtctc ctactgcaac
     9361 gcggtggaga acgtggtggt gcaggaaagt tatttcgccg cagccgacct gcgcgcggag
     9421 atcgcgcgcg agcaatacgg ggcgggcctg ttgaccttcg agaactggga cgtgatcgag
     9481 aacgaccgca tctcctatca gcgccagttg ctgcagtccc agcagcaggc cctgctggcc
     9541 gaggccgcgt ggcgctacgt cacgggccgg ggcgatttcc cggcggaaaa cgaggtgagc
     9601 cgatgaaaaa gctgctgctg ttcctggtcc tggctgccgc ggcgggcgtg ggctggtacg
     9661 ggtggaccca ccgcgagaaa gcggccgtga aggcgccgtc cttcgagccc gtcgagttca
     9721 cccgcgagcg gatcgaggtc gccatcgagg ccacgggcgt ggtcgagccc ggcaaccgca
     9781 tcgagatcaa gccgccgatc ggcgggcgcg tggaggaagt gctggtggtc gaggggcagg
     9841 ccgtcaggca gggcgaggtg atcgcccgga tgagttccac cgaccgcgcc gccctgctcg
     9901 acgccgcccg tgcgcagggc gaggccgtgc tgcgcaagtg ggaggaggtc tacaaggcca
     9961 cgcccctcat cgcgccgctg gacggcacga tcatcgcgcg caaggcggag cccggccaga
    10021 ccctgaccgc tgcggacacg gccctcgtgc tctccgaccg gctcatcgtg cgggcgcagg
    10081 tggacgagac ggacatcggc ctcgtccggg gagggcagga agcgacgctt acgctcgatg
    10141 cctatgcgga tgatcggttc cgaggccagg tcaggcacat cgcttacgag gcgaccaccg
    10201 tgaacaacgt cacgatctac gaggtcgagg tcgagcccgc gaccgtgccc gagcgcatga
    10261 agagcggcat gaccgcgacg gtcaagttcg tggtcgaggc ggcggacgac gcgctgaccc
    10321 tgccgctgga cgccctcgtg cgccgggacg ggcgcagcgt ggtcctgctc gcgccccgcg
    10381 gccccggggc gcgcccggaa gaacgcgaag tgaagaccgg tctgaccggc ggcggccggg
    10441 tgcagatcct ggaggggttg gacggcagcg agcgcgtggt gcgctcgtcc tttgacctgc
    10501 cgaaacgcca ggaatccgga agctcgccgt tcatgcctcg cccgcccggc cgacgcccat
    10561 gatagatgta cgcgacatac acaagaccta ccgcatgggc gccgtggacg tccgcgcgct
    10621 gcgcggggtg tcgctgtccg tggcgccggg cgagttcgtg gccctgatgg gcccgtccgg
    10681 ctcgggcaag tccacgctca tgcatatcct cggcctcctc gacgtgccgg acaagggcgt
    10741 ctaccggctg aacggacagg atgtctccag cctgtccgac cgcgaactgg ccgacgtccg
    10801 cgcgcgctcg atcggctttg tcttccagca atacaacctg ctcccgcgcg tgtccgcgaa
    10861 ggagaacgtc gccctgcccc tgatttacag cgaccagccg aatcccgcct cgccgaagga
    10921 actgctcgaa cgcgtcgggc tgggcgaccg catggaccac gcgccgaaca agctctcggg
    10981 cgggcagcag cagcgcgtcg ccatcgcccg cgcgctggtc aaccggccgc cgatcctcat
    11041 ggccgacgag ccgacgggca acctggacag ccgcagcgcg gtggagatcc tggggatcct
    11101 gcgcgacctg cacgcgtccg ggctgacggt gatgatcgtc acccatgatc ccgaggtcgc
    11161 cgcgcgggcg gaacgggtca tcaccatccg cgacgggcgg atcgtcggcg atgccccgac
    11221 cgccgggaag acccatcacg ctttgccggc ggggcccgac ctcgcgggtg cgtccgccgg
    11281 caaggggcgg aaaggctggg cgcgccggct ggtggaaggt ttttcgatga tgcgccaggc
    11341 gttcagagcg ctggcggcca acaagacccg cgcctttctc tccatgctgg gcgtgctgat
    11401 cggcaccggc gcggtgattg ccgtgctggc cctcggcacg ggcgcgaaga agtcggtcga
    11461 ggagcgcatc ggggcgatgg gcgcgaacct gctcgtcctg cggcccggcc gtatcgaggc
    11521 gcgcggcgtg gccatggaag tgggggcggc gtcgcggttg agcctggccg acgtggacgc
    11581 ggtccgcacg gaggtcgcgg gcgtcaagac cgtggcgccg acggtcggcg gcggagcgca
    11641 ggccacgttc ggcgggaaga actggcggac gcaaatcgtc ggcacggtgc cggcctacgc
    11701 gtcgatgcgc aacctgacgg tggccgtggg ccggttcctg gaagaggagg acgcgcggac
    11761 gcgcgcccgc gtcgcggtga tcggcgccac ggtctcgcgg gagcttttcg gcggcgtgaa
    11821 tcccgtcggc cagtccatcc gcgtcaaccg ggtcgccttc gaggtgatcg gcgtgctgac
    11881 cgagagcggc acgggattcc gattcgatcc gaacgaccag atcatcctcc ccgtcaccac
    11941 ggccatgcgg cgcatgctcg gccgcgacta cgtggacacg atcgagatcc agtcgacgga
    12001 cggcgacgtg ctctccgccg tcgagacgaa ctccgtcgaa ctgatgcgcc agcggcaccg
    12061 gatcagcccg gaggaggaag agccgttccg ggtcttcaat atggccgata tccagaagac
    12121 catcctcgac accagccgga cgctgtccct gctgctcgcg ggcatcgggg cgatctcgct
    12181 gctcgtcggc gggatcggca tcatgaacat catgctcgtc agcgttaccg agcgcacgcg
    12241 ggagattgga atccgcaagg cgatcggggc ccggcggtcg gacatcctgg tccagttcct
    12301 ggtcgaggcc gtggtcatca gcgtctgcgg cggcgtgctc gggattctca cggggtggag
    12361 catttccgtg gcgatgagcc ggcttgccgg ctgggcggta tccgtcacgg cgccgaccgt
    12421 gctgctggcc accgtgttct ccgccctcgt cggcgtggtc ttcgggcttt ggcccgcgcg
    12481 gaaagcctcc ggcctcaatc ccatcgaggc gctgcggtac gagtaggccc ttgtagaacc
    12541 gccggcctgt cgacgacaag gccaacggcc tgaatgcgag cgccgtggcg ggtatcggca
    12601 acgaccggtt cacgatggag tggtggatgc gcggccggcg cgatggcgtg gaccaatcct
    12661 ccttcagaca ccgcgccggt ccgctacttc cggctgggcg tcgaacggcc gtaggaagaa
    12721 gggcttgtcg ccacctcgaa gaccccccag gtgccgccgt cgcccgccac cggagtgaag
    12781 agcccgggat gcttcgtcat gaacgccagt tcgagcgact ccggggcgag gctcaccacg
    12841 tgggtgatcc cggcctgccc gagccggcgc agccatgcgc ggctgtcgac ccgatcgcgc
    12901 agttcctcga cggattgggc gtcgagaacc cggccggaag cggtcggcga aatgtaggtc
    12961 accgtgttct gaaaccgccg gccgaggaag aagtagagga accagttgtc gccgttgtag
    13021 tcgatgcccg tggtcacagc gagccggcgg gggacggcgg gatcgtcgag cgcccgcgcg
    13081 ccgtcggccc atggattctc gacccagtgc aggacgatcg attgcttgag ggccttgtag
    13141 cgcaggtgat cgcgttcttt ctggatggcc gtcaggctgg cggccaggag cgccgccgtc
    13201 acgacgccgg agacaacggc ccgccggttg atccgggaaa ggatgaccag cgaagccgcc
    13261 agggcggcca ggagcccggc ggatttcggt atgacgcgga attcgaacgg cgcccagccc
    13321 tggcaggctg cgcgcccggc atgcagcagc gcaaggagga tcagcgcggc ggccaggacc
    13381 agtcgaaggc gcggtttagt ccagagccag gcgagcccga ggagggaggc cgggacgaac
    13441 gcgggcagca ggaaccgcgc ggacgtcgtg ctccagagca gccggatgac cgcgaaggat
    13501 ggcccccaga acacccaggc cgtcaagccg atgaacccgg ccagcaacag ggcattggcc
    13561 gcgcccatgc gccggaagcg gaccagggaa atccatcccg ccacgcccag gggcagggtg
    13621 agcagcccca ggtgcggcca cgtcgatagc ggcgtcgcga agagatcgcg ccccgcctgg
    13681 atttcctcgc gcagatcgcc gtgtctcacc ccttcgcgat ggtggtaggt gtggtagctg
    13741 ggcgtggact cgcccaggac caggcccgcc acccggatcg gaaaaggaga aagcggcagg
    13801 cgcccggcgc ggagattcag ggccggccag ggcaggacca tgagcagggc cgccagtatt
    13861 ccagccagca gcgccggcct gcacttccgc gcggaagccc ggccacggca acaggcgccg
    13921 gccagcagga ccagtccggc ccaggccagg gccggccaga cgtgtgcctt gatcccggcg
    13981 gccatcccaa aagcggcgaa agcaatcacc agcgacggag gatccctctg gcgcaggaag
    14041 tgaatggaat acgacaggcc gccgagcagc gcgcacatca gcggcagctc tccatacccg
    14101 ctgccgattt gcaggcggag ggccggcagg aaaagaagat agagggcgcc cagcgcgccg
    14161 gccttcgtgg tcagccccag ttcccgcgcg aattgcacga tgaacgcgcc cagggccagc
    14221 cagaacacga aatcgagaaa gcccagcaga agatcgcttc ggaacgggag cagggcccag
    14281 gcctggagca actcgctgcc gccgggcagg agaatcttgc tgccccagcc gcccggcgcg
    14341 ggaaagaggt ccggccggcc ctgctgcgcc cagagcgcgg ccttcacgcc gtggtaggtc
    14401 agcgtatccc agccgagcgg gggaagcaga agcgtccgcg tcagggccag cagcatgaaa
    14461 agaaaaatca gggcggcgcc cgcgcgccgt gcccccgtcg aaagcaggag cgacccggcg
    14521 cggcggaacc gcgcgaggtc gtcgagccat cgctcccgcc attcggccgg cggaacgatc
    14581 ccttgtccga tccatcgggg cggcgttgtc cc
//
LOCUS       DAOOTK010000085        14565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2804_length_14565_cov_5.209028, whole genome shotgun sequence.
ACCESSION   DAOOTK010000085 DAOOTK010000000
VERSION     DAOOTK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_14565_cov_5.209028"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PK634_09800"
     CDS             <1..953
                     /locus_tag="PK634_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOW98069.1"
                     /translation="EAHMDTVGVTGMAEPFRARVENGRVHGRGACDCKGPMAAGLAAL
                     TPEALDGLVRKGYRVVFAAVMGEETGNAGAIRLVEQGLRADRAVILEPTELEIVHAHK
                     GALWFDVEVKGRAAHGSNPERGVNAIVGMARLLERIQKRHELDRARLTHERLGQPTLS
                     VGQIRGGTAVNIVPDQCCARLDRRTLPGEKGADILAGVRAELSDLVHEGVLLGGEVKV
                     IKEGPPFHTDEDSELVRALGRACEKAGLPARLAGSGWFSDAGPLSTACPEVVVFGPGS
                     ISQAHTCDEFIEVESLRRGVAVLAALCRELAPADSTRKAK"
     gene            955..2577
                     /locus_tag="PK634_09805"
     CDS             955..2577
                     /locus_tag="PK634_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98070.1"
                     /translation="MIRMSWKLGLGLGALVLYVALAGCNLFWGDLNQDEGWYLYAAGL
                     VRDGQVPYRDFAFTQGPVLPYVYAEVYSWVDRWGVMGGRLFTALLGGLAALGAAALAA
                     RATPSGWKCAAGLLAMTLLAGNVYQSYFTTVVKTYALSSLFLVGGLLALSYAGGRLRA
                     AAAFAAGVLLALSAGTRLSAGAALPIAGVYLLLQRRRLGNAPWLMFGIGGGLALAGLS
                     APFLWCAPEGFRFGLFEYHSARAPGTLAQLLVFKAGFVSRTVQAYFLPLLLGLGLAGL
                     RWITPAEKTAGLPGGGTPGGFGGALWATVALVTLVHLAAPFPYDDYQAMVFPVFASAL
                     AAAAARYLAEVRARAAAPAGEADPWLAWLLALVFVGSVASAFSSPVNQDWFVLGRDRI
                     WWRLKQDSALQKVRDIGAWLGKKSRIGDVLLTQDTYLAVEARLLVPMGMEMGPFSYFP
                     DMPTERARALRVLNRELMAEQLTAAPASFAAFSGYGLAIRSPEVAPLTAAEQAELWKL
                     VRLRFAEFCQVPDFGQAHTVLRLFRRLPEDRP"
     gene            2574..3341
                     /locus_tag="PK634_09810"
     CDS             2574..3341
                     /locus_tag="PK634_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW98071.1"
                     /translation="MKLSIVVPAFNEEARLGPMLDTYVAWFAPRHGTDMEFVVVVNGS
                     TDRTEHIAREAAARCPQVRAVIEPRAIGKGGALLLGFGEARGDLVGFVDADTSTPPEA
                     FQDLVDHIGDAGAIIASRWMPGSQVSPRQPLARRFASRIFNRLVRLLFGLRITDTQCG
                     AKLMRGDALRRVMPLLGITRWAFDVDLLFQLKRAGYRIVEWPTVWRDAGGSRLKVGRA
                     SLEMFIAICRLRLLFSPFRWVVTVYDRTLGPFIRVKG"
     gene            3344..4618
                     /locus_tag="PK634_09815"
     CDS             3344..4618
                     /locus_tag="PK634_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98072.1"
                     /translation="MANRGQEHLLRHGVLLMAATQVANVSNLLFQVVMGRTLSAEQYG
                     ELASMLGIILVLATPMDAVRTAVAHFASRLLGEGRTGEIRVLVGRGFRMMTWAAAPVL
                     LVSLLLGDVLARWFHLDSGLPVRLTGLIVAGSFYMPVFIGSLQGVQSFGWMACTQHSW
                     SVVRLVAGGALVWSVAPLATWGLAGHLAGVLSSIVWGAIGLRVLPSRERSDAGTAPIA
                     DVFRYFVFSLLILGGFAVLMNADIIMVKHLFSPLEAGLFARGATIGRSVVFVSVPIAM
                     AMFPKVTSAGEVAAGTRGTLVRAFVFSGLIVLLAVGAVTLWPALPLRILFRVAEPDLQ
                     MIGLVRALTWAMAPLGLVYLAVHFELAQHRFRSVLPLLVCATAYAAAVALRHERVEQV
                     AQALAACSWLALAWLLVGLPWRAKDLPSGGAS"
     gene            4615..5181
                     /locus_tag="PK634_09820"
     CDS             4615..5181
                     /locus_tag="PK634_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOW98073.1"
                     /translation="MNGTNSGRPCVFFDRDGIVNRSPGPGYVERVEDFHLLPEFVEAL
                     RVVRARGYEAVIVTNQRGVALGRMSAETVQSMHDELARRLRAEGLDLLDIRVCTANDN
                     RHPDRKPNPGMLLAAARRHGLDLGRSWMVGDHERDIEAGRRAGCRTVRVGEPGQPSAA
                     DFRVDDMRGLAAFLESHLQGFPRPPAGC"
     gene            5236..5841
                     /locus_tag="PK634_09825"
     CDS             5236..5841
                     /locus_tag="PK634_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOW98074.1"
                     /translation="MGIERAEQYLETVVRAARRVDLRMVERFLDLLESAYRDGRAVYV
                     IGNGGSAANASHFAEDLSMGAMPDLEGKRFRVLSLTDNTSFITALANDKGYDRIFELQ
                     LRQFGQKGDLLVVISGSGNSPNVVRASEYAKSIGMTVVALTGFDGGKMKGLADLNLHV
                     PHPHMCQSEAVHSILQHVVADLLRERLSAPAPRPVTRGRER"
     gene            5849..7891
                     /locus_tag="PK634_09830"
     CDS             5849..7891
                     /locus_tag="PK634_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW98075.1"
                     /translation="MGFRTQPVPAPAPSRFTPGMFLVAALLLAAFALRFARLGYTLLW
                     IDELDVFWNSSSPRGPLDVILYTLQNCFEINTNEQMPFQYAWLKLFLALYRALGIEQP
                     GIALVRLPFALLGFLSVGVLYAAVRRLYDRATAAWTLALAALSFFHVFQSRDATSYNP
                     LMFFMALHFWGAAGWLRPGRPRWTDRLGFIAGAAGMLMSHLSSWLFLGPAGLAWLGRA
                     AWLRWRSAEKRPWGRELIGGAFEPAALLVLCALPFLIVIYRAVTVFKPPGLKEEVEIS
                     TLSLPFLRYQLAHFGWGLGVGRLVAFLLALAGGVAGLLGERSTRARGAIHLALLGASA
                     ALLFIAPHALIFPRYLAIAFLPMLVVAGRGLAWWTQGASRRLKLAGRAAWAPAAVAAA
                     LLAAWHVRPFAQLMTLRTKLMPVPQLIEWLETQLPDNGLYVWESGFNLRDVPGAYPVR
                     GRQAAFMTMHSGSIRVPAVAEQLAPMGRFLFQRFPLAALLATGFPEPPAEGPWSWIGA
                     DFARRDVVRNESLERLHRYGFSPHGYQYPSNLNIVCYFNRPEDLPSRLGADGRPFLVY
                     PDGPGWKYLQTREGLLFACPEPAGALRVRNLSGQARSGRLVIEGAVQALGSLRVTGPG
                     GQVLTKNLQPANHIREVFGPFSFPTGEQEVRIEFWPAGRNALLLYAFQVGPGESGP"
     gene            7903..9426
                     /locus_tag="PK634_09835"
     CDS             7903..9426
                     /locus_tag="PK634_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOW98076.1"
                     /translation="MPEAKILDLDKLASVVAKARAQGKVVVHCHGVFDLLHIGHIRYF
                     EQARRMGDVLVVTLTPDQYVDKGPHRPAFTQDLRAEAIASLSCVDHVAINRWPTAEET
                     LRLLRPDLYVKGSEFKEITSDMTGKIGREEKVVREVGARLAFTEDIVFSSSNLINTHM
                     SLFPVEVKEYLNLFKTRYSLNAVNEALSRLSKLKVLVIGDTIIDTYVYCEAIGKSSKD
                     PVLALRHQSEDHFAGGVLAVANHVANFAGHVELFTVLGAVNTRQEFITSSLASNVTPH
                     FFLRPGAPTIVKQRFLEGYSLSKLLEIYYMDQSDIPDETIREVTAALKEKLKECDLVV
                     VADYGHGMMAKPIRHLLCEEAKFLAVNTQANAGNRGFNTITRYPRVDFGCIAEHEMRL
                     EMRDAEGELRPMIEKLAPQLKSRVFVVTRGKKGALIWTGEGEFVIVPAFAMKVVDRVG
                     AGDAFLSVTALAAATGVPPEVLGFIGNVAGAQAVEIIGNQKAIDKLKLQKTIVSMLK"
     gene            9430..10437
                     /locus_tag="PK634_09840"
     CDS             9430..10437
                     /locus_tag="PK634_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98077.1"
                     /translation="MRAEPPHRPPADLFDRSRLQIRPLAERKHDLGLDRIRPLESVLS
                     IEPALRAVAARMLAARDAGAAIVLMMGAHVLREGMQRYLMDLMKRRLVTLVALNGAGL
                     IHDFELALIGQTTESVARYIAEGQFGLWQETGRINDLVAAAARNGLGLGEAVGAAIAE
                     GAFPHKDISLLAAAYRLGVPVTVHVGIGYDIVYEHPNCDGAAWGAASYTDFLRFTKGL
                     ERLEGGVVMNFGSAIMAPEIYLKALAMVRNAARPEGRTIAHFATLVCDLHDLPADFHA
                     EAPRGAAGYYYRPWKTMLVRTVADGGESFYVRGHHAQTVPELWSCLNELEAARARSKP
                     S"
     gene            10534..13293
                     /locus_tag="PK634_09845"
     CDS             10534..13293
                     /locus_tag="PK634_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOW98078.1"
                     /translation="MNRLRRWVSWAGSLCMCLAPAYARGADLSTPAGAEQAIADVVRE
                     IEAKVKLSIGQLSVSPPPVQPELRVLEQYVDEYLRRHGDRVSVANPIWALTNACNAQA
                     VQRDYDRIVPVGKQHNAYVEVNNKLKEKYDQLLQLSAYEVLAKGIGLILFPPGSGLDI
                     LQEIGMEAIENCMTPSFRPLGAERLRSTTVPRLAAELKPEQEKLLVALREAPASKNDV
                     PRICKAAQQLAASCEEKAGRIYDELLKQHEEVKAQAPTIFVNVVAVATEVDRILKRRA
                     RITTCEVLPSPLVLPGVGAFYPLTLRLVYDSGLRKEAFYCDVVFEQDDPPVIRLFSNP
                     GSGHVIAESTAVGTSEVRVRSKDDGRLLGSFRVEVVSGSAPAPIPPAGPGQGAPRQAG
                     QAPPPAGGRPVVDFAVSPDQSVYAVGQSIRFTQGAVSNTADWDFIWYLTGAEKTGPEI
                     EHAFSSPGKHAVRLVVKERGGAWEDAMARFITVEETAGEGVLPPLQPGGTVTSAPPSG
                     PIGNVNDFVTETVRGKGVVAVRYWRGGAGDWSDPVAFLETGAAQELALQTGEQADGYN
                     TGWLLCVENGQPRFKVLGCDFERRTGRVVYEGALDLQGQDYKTGSLRFKAVHARCAEV
                     TWEAANGSLYGATIWKSPTPAAPDPNRFVKTGVEYEKPIELREPTGANQLTIGGSGTN
                     LCVTVPAGMMNVTASMQLSTGVKALGAVAADGVFVRLEDMPRETGREFVFSLSPGRHF
                     LPYPDTRGLPADARAVDVKVFDNATKALLRRHLAPLARSETASPLRKDEAPGPVLSEF
                     RYGSSAWPVRLQDGVLEVRGNGRWEWGEQWKLVDRYVKEFNGVGTDQGPALAHTKDGH
                     FWDVVLLNYQNGDRVPVNSYSEKVEIYYGGQYGFSYRVGGHSFDFSHGILKEIDPATG
                     NFLRP"
     gene            13312..13758
                     /locus_tag="PK634_09850"
     CDS             13312..13758
                     /locus_tag="PK634_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98079.1"
                     /translation="MKTNLWVTAGMAWAMTSCSGEKGPFREIRAWDSREMPYAMVFCA
                     AATNQDEAAVRREADRIAANKLRQSERVVVVILYDADRARDVSSEKAMRNMMSGAGAG
                     VNALLYGGRFEGGGFLSAAREGTTPAWTFVPFRDTGASARDKAFSR"
     gene            13778..>14565
                     /locus_tag="PK634_09855"
     CDS             13778..>14565
                     /locus_tag="PK634_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98080.1"
                     /translation="MKTRTFLGGLGLAIATCLAAGSDDAGPRIVPADLDAALAWASDT
                     AAWEGLRGARYVTAISLKAGAAEGMTALVRKELAPPGPPDGPEPQETAGSIEWIKYDV
                     SLLVHVSAESADAALDGLLAGDRRPFAAGEEGYVVVGDGVLFPEGGEAVSQGHRTAAF
                     RAGRYNAVIQQDWQGRRELPEGPDPVRSLLDEDLRSLAQALAERAAAASESAAPEYSV
                     LFDKLQDLEARGDRLGALRVIPSVFECDIPVDAFQETLAATRAR"
BASE COUNT         2072 a         4808 c         5268 g         2417 t
ORIGIN      
        1 tcgaggccca catggatacc gtcggcgtga ccggcatggc cgagccgttc cgcgcccggg
       61 tcgagaacgg ccgtgtccac ggccgcgggg cctgtgactg caaggggccc atggccgcgg
      121 gactcgccgc cctgacgccg gaggccctgg acggactggt ccggaaggga taccgcgtgg
      181 tctttgcggc ggtcatgggc gaggagacgg gcaacgcggg cgcgatccgc ctggtggagc
      241 aggggctgcg cgcggatcgc gcggtgatcc tggagccgac ggaactggag atcgtccacg
      301 cgcacaaggg cgccttgtgg ttcgacgtcg aggtgaaggg ccgcgccgcg cacggctcga
      361 atcccgagcg gggcgtcaac gcgatcgtgg gcatggcgcg cctgctggag cgcatccaga
      421 agcgccacga gctggaccgg gcccgactga cccacgagcg gctcggtcag cccacgttgt
      481 ccgtcgggca gatccggggc ggcacggcgg tcaacatcgt gccggaccag tgctgcgccc
      541 ggttggaccg ccggacgctg ccgggcgaga agggcgccga tatcctggcg ggcgtccggg
      601 ccgagttgag cgatttggtt cacgagggag tgttgctggg cggcgaggtg aaggtcatca
      661 aggaagggcc gccgttccac acggacgaag attcggaact cgtgcgcgcg ctggggcgcg
      721 cgtgcgaaaa agccggtctt ccggcccggc tcgcggggtc gggctggttc agcgacgccg
      781 ggccgctttc cacggcctgc ccggaagtgg tagtgttcgg gccgggcagc atcagccagg
      841 cgcacacctg cgacgagttc atcgaggtgg agagcctgcg ccggggcgtg gcggtcctcg
      901 cggcgttgtg ccgggaactg gcgccggcgg attcgacacg gaaggcgaag tgacgtgatc
      961 cggatgtcat ggaagctggg attgggcctg ggcgcgctgg tcctgtacgt cgcgctcgcg
     1021 gggtgcaacc tgttctgggg cgatctgaac caggacgagg gctggtacct ttacgccgcc
     1081 gggctcgttc gcgacgggca ggtgccgtac cgcgatttcg ccttcacgca ggggcccgtg
     1141 ctgccctacg tgtacgcgga ggtctactcg tgggtggacc ggtggggcgt catgggcgga
     1201 cggctgttca ccgcgctcct cggggggctg gccgcgctgg gcgccgccgc gctcgcggcc
     1261 cgcgcgacgc cgtccggctg gaagtgcgca gcgggcctcc tggccatgac gctcctcgcc
     1321 ggcaacgtct accagagcta cttcaccacg gtggtcaaga cgtacgcgtt gagctcgctc
     1381 ttcctggtcg gcggcctgct ggcgttgagc tatgccggcg ggcggctccg ggcggccgcc
     1441 gcgttcgcgg ccggggtgct gctggcccta tccgccggaa cccggctctc cgcgggcgcc
     1501 gcgctgccga tcgcgggcgt ctacctgctg ctccagcggc ggcggctcgg gaacgcgccg
     1561 tggctgatgt tcggcatcgg cggcggcctc gcgctggccg gcctgtccgc cccgttcctg
     1621 tggtgcgcgc cggaaggctt ccgcttcggc ttgttcgagt accattccgc gcgcgccccc
     1681 ggcacgctgg cgcaattgct cgtgttcaag gccggcttcg tctcgcgcac ggtccaggcc
     1741 tacttcctgc cgctgctgct gggcctgggg ctggcgggcc tgcggtggat cacgcccgcg
     1801 gagaaaacgg cgggcctccc cggcggcggg acgcccggcg ggttcggcgg ggccctctgg
     1861 gcgacggtcg cgctggtgac gctcgtgcac ctggccgcgc cgttcccgta cgacgattac
     1921 caggcgatgg tcttcccggt gttcgcgtcg gcgctggccg ccgcggcggc ccgctacctg
     1981 gccgaggtgc gcgcgcgcgc ggccgccccg gcgggggagg cggatccctg gctggcctgg
     2041 ctgctggcgc tcgtgttcgt cggctcggtc gcgtcggcct tttcgtcgcc ggtcaaccag
     2101 gactggttcg tgctgggccg cgaccggatc tggtggcggc tgaaacagga ttccgcgctg
     2161 cagaaggtcc gcgacatcgg cgcctggctg gggaagaaga gccggatcgg cgacgtgctg
     2221 ctgacgcagg acacctacct cgcggtcgag gcgcggctgc tcgtgccgat gggcatggag
     2281 atgggcccgt tcagctactt ccccgacatg cccaccgagc gggcccgcgc gctccgcgtg
     2341 ctgaaccggg agctgatggc cgagcagctg acggcggcgc ccgcgagctt cgccgccttc
     2401 agcggctacg gcctggccat ccgttcgccc gaggtggccc cgctgaccgc cgcggaacag
     2461 gccgagctgt ggaagctcgt tcggctgcgc ttcgcggagt tctgccaggt ccccgatttt
     2521 ggccaggccc acacggtcct tcggctcttc cgccggctgc cggaggatcg tccgtgaagc
     2581 tgtccatcgt cgtgcccgcc ttcaacgagg aagcccggct cgggccgatg ctggatacct
     2641 acgtggcctg gtttgcgccc cgccacggga ccgacatgga gttcgtcgtt gtggtcaacg
     2701 gctcgactga ccgcacggag catatcgccc gggaagccgc agcgcgttgt ccgcaggtgc
     2761 gggcggtgat cgagccgcgc gcgatcggca agggcggggc gctgctgttg ggtttcggcg
     2821 aggcccgggg ggacctggtc gggttcgtgg acgcggatac gtccacgccg ccggaggcgt
     2881 tccaggatct ggtggatcac atcggcgacg ccggggcgat catcgcgtcg cgttggatgc
     2941 ccggctcgca ggtcagcccc cgccagccgc tggcgcgccg cttcgcctcg cggatcttca
     3001 accggctcgt gcggctgctg ttcgggctgc ggatcacgga tacgcagtgc ggggccaagc
     3061 tgatgcgggg cgatgcgctg cggagggtga tgccgctgct gggcatcacg cgctgggcgt
     3121 tcgacgtgga cctgctgttc cagctcaagc gggccggcta ccggatcgtc gagtggccga
     3181 ccgtgtggcg ggacgccggc ggctcgcgcc tgaaggtcgg acgcgcctcg ctggagatgt
     3241 tcattgccat ctgccggctg cgcctgctgt tctccccgtt ccggtgggtc gtgacggtat
     3301 atgaccggac gctgggcccc ttcatccggg tgaagggctg accatggcga atcgcggcca
     3361 ggagcatttg ttgcggcacg gcgtgctgct catggcggcc acgcaggtgg cgaacgtctc
     3421 caacctgctg ttccaggtgg tcatggggcg cacgctgtcg gccgagcagt acggggaatt
     3481 ggcttccatg ctgggcatca tcctcgtgct ggccaccccg atggacgccg tgcgcacggc
     3541 cgtggcgcac tttgcgtctc ggctgctggg ggaagggcgg acgggtgaaa tccgggtgct
     3601 tgtggggcgg ggatttcgga tgatgacgtg ggcggcggca cccgtgctac tggtctccct
     3661 tctcctgggg gacgtgctgg cccggtggtt ccacctggat tcgggactgc ccgtccggct
     3721 gacggggttg atcgtggcgg gctcgttcta catgccggtc ttcatcggat ccttgcaagg
     3781 ggttcaatcc tttggctgga tggcctgcac gcagcacagt tggagcgtcg ttcgcctcgt
     3841 ggccggaggg gcgctggtct ggagtgtagc ccccctggcg acctggggac tggccggcca
     3901 tctcgcgggc gtgttgtcca gcatcgtctg gggcgcgatt ggcctccgcg tcctgccgag
     3961 ccgcgagcgg agcgatgccg ggacggcccc catcgcggat gtcttccgct attttgtgtt
     4021 ttccctgctc atccttggcg ggtttgccgt gctgatgaac gcggacatta ttatggtgaa
     4081 acatctcttc tccccgttgg aggccggtct gttcgcgcgc ggggcgacga tcggccgatc
     4141 cgtggtgttc gtctcggtac ctatcgccat ggccatgttc ccgaaggtga cgtcggcggg
     4201 ggaggtggcc gccggcacgc gaggcacgct ggtccgagcc ttcgtattct ccggcctcat
     4261 tgtgctgttg gcggtcggcg ccgtcacgct atggccggct cttccgctgc gaatcttgtt
     4321 ccgcgtggcg gagccggatc tccagatgat cggactggtc cgggccttga cgtgggccat
     4381 ggcgccgctc ggcctggttt acctggccgt gcatttcgaa ctggcgcagc accggttccg
     4441 ttccgtcctc ccgctgttgg tctgcgcgac agcctacgcg gctgccgtgg cgctgcggca
     4501 cgaacgcgtc gagcaggtcg cgcaggcgct ggccgcctgt tcctggctgg cgctggcgtg
     4561 gttgcttgtc gggctgccct ggcgggcgaa ggacttgcct tcgggaggcg cgtcatgaac
     4621 ggaacgaact cgggccgccc ctgcgtgttc ttcgaccgcg acggcatcgt caaccgttcg
     4681 cccggccccg gctacgtcga gcgcgtggag gattttcacc tgctcccgga attcgtcgag
     4741 gcgctgcgcg tggtccgggc ccgcggctac gaggccgtga tcgtgacgaa ccagcggggc
     4801 gtcgcgctgg gccggatgag cgcggagacg gtgcagtcca tgcacgacga gctggcccgg
     4861 cgactgcgcg ccgaggggct ggacctgctg gacatccggg tctgcacggc gaacgacaac
     4921 cgccaccccg accgcaagcc gaatcccggc atgctcctgg cggcggcccg gcggcacggg
     4981 ctcgacctgg gccgctcgtg gatggtgggc gaccacgagc gggacatcga ggcgggccgg
     5041 cgggcgggct gccgcacggt ccgggtcggc gagcccggac aacccagcgc ggcggatttc
     5101 cgcgtggacg acatgcgcgg tctcgccgcg ttcctggagt cgcacctgca gggcttcccg
     5161 cgcccgccgg ccgggtgtta acccttgccg cgagcggtcc ggcggattat agtgccgggg
     5221 caatcggagt ggcctatggg catcgagcgg gcggaacaat acctggagac cgttgtgcgg
     5281 gcggcgcgcc gcgtggacct gcggatggtc gagcgcttcc tggacctgct ggaatccgcc
     5341 taccgggacg gccgcgcggt ctacgtcatc ggcaacggcg gcagcgcggc caacgcctcc
     5401 cattttgccg aggacctctc catgggcgcg atgcccgacc tcgagggcaa acgcttccgc
     5461 gtgttgagcc tgacggacaa cacctcgttc atcaccgccc tggccaacga caagggctat
     5521 gaccgcatct tcgagctcca gctgcggcag ttcgggcaga agggcgacct cctcgtggtg
     5581 atcagcggat ccgggaacag ccccaatgtc gtgcgcgcgt cggagtacgc gaagtcgatc
     5641 ggcatgaccg ttgtcgcgct gaccggcttc gacggcggga agatgaaggg cctggcggac
     5701 ctgaacctgc acgtgccgca tccccacatg tgccagtcgg aggccgtgca ttcgatcctt
     5761 cagcacgtgg tggccgacct gctgcgcgag cgtctgtccg cgccggcgcc gcggcccgtg
     5821 acgagaggtc gcgagcgata aggccgcgat gggcttccgg acccagcccg ttcccgcgcc
     5881 ggcgccgtcc cggttcacgc ccggcatgtt cctggtggcc gccctcctgc tggcggcctt
     5941 cgcgctgcgt ttcgcccggc tgggctacac gctgctgtgg atcgacgaac tggacgtctt
     6001 ctggaattcc agttcgccgc gcggcccgct ggacgtgatc ctgtacaccc tgcagaactg
     6061 cttcgagatc aacacgaacg agcagatgcc cttccagtat gcctggctca agcttttcct
     6121 ggcgctctac cgggcgctgg gcatcgagca gcccggcatc gccctcgtgc ggctgccgtt
     6181 tgccctgctg ggtttcctct ccgtcggcgt cctgtatgcc gcggtccggc gcctgtacga
     6241 ccgggcgacg gcggcctgga cgctggccct ggcggccctc tcgttcttcc atgtctttca
     6301 gagccgcgat gccacgagct acaacccgct gatgttcttc atggcgctgc atttctgggg
     6361 cgcggccggc tggctgcgcc cgggcagacc ccgctggacg gaccggctgg ggttcatcgc
     6421 gggggccgcg ggcatgctga tgtcgcacct gtcctcgtgg ctgtttcttg gcccggcggg
     6481 gctggcctgg ctgggccgcg cggcctggct ccggtggcgg agcgcggaga agcgaccgtg
     6541 gggccgggaa ctgatcggcg gcgccttcga gcccgccgcg ctgctggtcc tgtgcgccct
     6601 gccgttcctg atcgtgatct accgggccgt cacggtgttc aagcctccgg gcctgaagga
     6661 ggaggtcgag atatcgacgt tgtcgctgcc gttcctccgc taccagctcg cgcacttcgg
     6721 ctgggggctg ggcgtcgggc gcctggtcgc gttcctcctg gccctggcgg gcggcgtcgc
     6781 gggcctgctg ggcgagcggt cgacccgcgc ccgcggcgcc atccacctgg ccctgctggg
     6841 cgcctcggca gcgctgctct tcatcgcgcc gcatgcgctg atcttcccgc ggtacctggc
     6901 gattgctttc ctgccgatgc tggtcgtcgc cggccgcggc ctggcgtggt ggacgcaggg
     6961 cgcgagccgc cgcctcaagc tggccggacg cgcggcctgg gcgccggccg cggtcgcggc
     7021 ggcgctgctg gcggcgtggc acgtccggcc gttcgcgcag ttgatgacct tgcggacgaa
     7081 gttgatgccg gttccgcagc tgatcgagtg gctggagacg caactgccgg acaacggatt
     7141 gtacgtctgg gagtccgggt tcaacctgcg cgacgtgccg ggcgcctatc cggtccgcgg
     7201 gcggcaggcg gcgttcatga ccatgcactc cgggtccatc cgtgtgccgg cggtcgcgga
     7261 gcagctcgcg cccatgggcc ggtttctctt ccagcggttc ccgctcgccg cgctgctggc
     7321 cacgggcttc ccggagccgc ccgccgaagg cccctggtcg tggatcggcg cggatttcgc
     7381 ccgccgggac gtcgtccgga acgagagcct ggagcgattg caccgctacg ggttcagccc
     7441 gcacgggtac caatacccgt ccaacctcaa catcgtctgt tacttcaacc ggcccgagga
     7501 cctgccgtcg cgcctcgggg cggacgggcg gccgttcctg gtttatccgg acgggccggg
     7561 ctggaagtac ctgcaaaccc gggagggcct tctcttcgcg tgcccggaac cggcgggcgc
     7621 tctccgggtc cgcaacctct ccggccaggc gcggtcgggc cggctggtga tcgagggcgc
     7681 cgtgcaagcg ctcggcagcc tccgcgtgac cggaccgggc gggcaggtct tgacgaaaaa
     7741 cctgcaaccc gctaatcaca tcagggaggt gttcgggccg ttctcgtttc cgaccggcga
     7801 gcaggaggtg cggatcgagt tctggccggc cggccggaac gcgctgttgc tgtacgcgtt
     7861 ccaagtgggc cccggcgaaa gcgggccata aaggagtcgg gcatgcctga agcaaaaata
     7921 ctggatctgg acaagctggc gtcggtggtg gccaaggccc gcgcgcaggg caaggtcgtg
     7981 gtgcattgcc acggcgtgtt tgacctgctg cacatcgggc acatccgcta cttcgagcag
     8041 gcccgccgca tgggggatgt cctggtcgtc acgctgacgc cggaccagta cgtggacaag
     8101 gggccgcacc gcccggcctt cacccaggac ctgcgcgcgg aggccatcgc ctcgctctcg
     8161 tgcgtggacc atgtggccat caaccgctgg ccgaccgccg aggagacgct gcgcctgctc
     8221 cggccggacc tttacgtcaa gggctccgag ttcaaggaaa tcaccagcga catgaccggc
     8281 aagatcgggc gcgaggagaa ggtggtgcgc gaggtcggcg cccgcctggc cttcaccgag
     8341 gacatcgtct tcagctcgag caacctcatc aacacccaca tgtccctgtt cccggtcgag
     8401 gtgaaggagt acctgaacct cttcaagacc cggtactcgc tgaacgcggt caacgaggcg
     8461 ctcagccggc tctcgaagct caaggtcctc gtgatcgggg acacgatcat cgacacctat
     8521 gtctactgcg aggcgatcgg caagtcgtcc aaggaccccg tgctggccct gcggcaccag
     8581 tccgaggacc acttcgcggg cggcgtgctg gcggtggcca accacgtggc caacttcgcg
     8641 ggccacgtgg agctgttcac cgtgctgggc gcagtcaata cgcggcagga attcatcacc
     8701 tcgagcctgg cctccaacgt cacgccgcat ttcttcctgc ggcccggcgc gccgacgatc
     8761 gtgaagcagc gcttcctcga gggctactcg ctgtccaagc tgctggagat ctactacatg
     8821 gaccagtccg acatcccgga cgagacgatc cgcgaggtga cggcggccct gaaggagaag
     8881 ctgaaggagt gcgacctcgt ggtggtcgcg gactacgggc acggcatgat ggccaagccc
     8941 atccgccacc tgctgtgcga ggaggcgaag ttcctggcgg tgaacacgca ggccaacgcg
     9001 ggcaaccgcg gcttcaacac gatcacccgc tacccgcgcg tggatttcgg ctgcatcgcc
     9061 gagcacgaga tgcgcctgga aatgcgcgac gccgagggcg aattgcggcc gatgatcgag
     9121 aagctcgcgc cgcagctcaa gagccgggtc ttcgtggtga cccgcggcaa gaagggcgcg
     9181 ctgatctgga ccggcgaggg cgagttcgtg atcgtgccgg ccttcgccat gaaggtggtg
     9241 gaccgcgtcg gggcggggga tgccttcctg tccgttacgg cgctggccgc cgcgaccggc
     9301 gtcccgccgg aagtcctggg cttcatcggg aacgtggccg gggcccaggc ggtcgagatc
     9361 atcggcaacc agaaggccat cgacaagctc aagctccaga agaccatcgt ctccatgctc
     9421 aagtgaccca tgcgcgccga gccgccccat cgcccgcccg ccgacctgtt tgaccggagc
     9481 cgcttgcaga tcaggcccct ggccgaacgg aagcacgatc ttggtttgga ccgcatccgg
     9541 cccctcgagt ccgttttgag catcgagccg gcgctccgcg cggtcgcggc gcggatgctg
     9601 gcggcccgcg acgcgggcgc cgccatcgtg ctcatgatgg gcgcgcacgt gttgcgcgag
     9661 gggatgcagc gctacctgat ggacctgatg aagaggcggc tggtcaccct cgtggccctg
     9721 aacggggccg gcctgatcca cgatttcgag ctggcgctga tcggccagac cacggagagc
     9781 gtggcgcgat acatcgccga ggggcagttc ggattgtggc aggagacggg ccggatcaac
     9841 gacctggtcg ccgccgccgc ccgcaacggg ctgggcctgg gcgaggccgt cggcgccgcc
     9901 atcgccgagg gcgccttccc gcacaaggac atcagcctgc tggcggcggc ctaccggctc
     9961 ggcgtgcccg tcaccgtgca tgtcggcatc ggctacgaca tcgtctacga gcatcccaac
    10021 tgcgacggcg cggcgtgggg cgcggcgagt tacacggatt tcctgcggtt cacgaagggg
    10081 ctggagcggc tcgagggcgg cgtggtgatg aacttcggca gcgccatcat ggcgccggag
    10141 atctacctga aggccctggc catggtccgc aacgcggcgc ggccggaggg tcggacgatc
    10201 gcgcatttcg cgacgctggt ctgcgacctg cacgacctgc cggcggactt ccatgccgag
    10261 gccccgcgcg gcgccgccgg gtactactac cgcccgtgga agaccatgct cgtccgcacc
    10321 gtcgccgacg gcggcgagag cttctacgtg cgcggccacc atgcccagac cgttcccgag
    10381 ctctggagct gcctgaacga gctcgaggcc gcccgcgccc ggtcgaaacc gtcgtaggcc
    10441 ggcccggttt cccgcgacgc cccggggctc aaagcagagt tgcctgccgc cgcgcggaag
    10501 gagtatcctg aagcaggaag gggggctcat ccgatgaaca ggctacgacg gtgggtgtcg
    10561 tgggcgggtt ccctttgcat gtgcctagcc ccggcctacg cccggggcgc ggacctgtcc
    10621 accccggccg gcgccgaaca ggcgatcgcc gacgtggtcc gggagatcga ggccaaggtc
    10681 aagctttcca tcgggcaact ttccgtgtcg ccgcctcccg tgcagcccga actccgcgtg
    10741 ctggagcagt acgtcgacga gtacctgcgc cgccacggcg accgggtctc cgtcgccaac
    10801 ccgatctggg ccctgaccaa tgcctgcaac gcgcaggccg tgcagcgcga ctacgaccgg
    10861 atcgtgccgg tgggcaagca gcacaacgcc tacgtcgagg tcaacaacaa gctgaaggag
    10921 aagtacgacc agctgctcca gttgtccgct tacgaggtgc tggccaaggg gatcgggctg
    10981 atcctgttcc cgccggggag cggattggac atcctccagg agatcggcat ggaggcgatc
    11041 gagaactgca tgacgccgag cttcaggccg ctcggcgccg agcgactgcg gtccacgact
    11101 gttccgcgac tggcagcgga gttgaaaccg gagcaggaaa aactgctggt ggccttgagg
    11161 gaggccccgg cgagcaagaa cgatgtgccc cgcatctgca aagccgccca gcagctggcg
    11221 gcgtcctgcg aggagaaggc ggggcgaatc tatgatgaac tgttgaagca gcacgaagag
    11281 gtcaaggcac aggcgcccac gatcttcgtc aacgtggtgg ccgtcgccac cgaggtcgac
    11341 cgtatcctca agcggcgcgc ccgtatcacg acgtgcgagg tattaccgag ccccctggtc
    11401 ctgcccggcg ttggcgcctt ttacccgctg accctccggc tggtctacga cagcggcctg
    11461 cgcaaggagg ccttctactg cgacgtggtt ttcgagcagg acgatccccc ggtgatccgg
    11521 ttgttctcca acccggggag cggccacgtg atcgcggaga gcacggcggt gggaaccagc
    11581 gaggtgcgtg tccggtcgaa ggacgacggc cggctgttgg gctcgttccg cgtcgaggtc
    11641 gtgagcggtt cggcgccggc cccgatcccg cccgcggggc ccggccaggg ggcccctcga
    11701 caggcggggc aggcgccgcc gccggcgggc ggaaggcccg tcgttgactt cgccgtgtcg
    11761 ccggaccagt ccgtgtacgc cgtcggccag tcgatccggt tcacgcaggg ggcggtatcg
    11821 aataccgcag actgggattt catctggtat ctgaccggag ccgagaaaac agggccggaa
    11881 atcgaacatg cgttttcatc tccgggcaag cacgccgtcc gtctcgtcgt gaaggagagg
    11941 ggcggtgcgt gggaagacgc gatggcccga ttcatcaccg tggaagagac cgcgggcgag
    12001 ggcgtcctcc cgcctcttca gccgggaggg acggtgacct ccgcgcctcc gtccggcccg
    12061 atcggcaacg tcaacgactt cgttaccgaa acggtccgcg gcaaaggcgt cgtggccgtc
    12121 cggtattggc gcggcggcgc tggggattgg tctgatcccg tggccttcct ggagaccggg
    12181 gcggcgcagg aactggccct gcaaacgggc gagcaggcgg acggctacaa caccggctgg
    12241 ctgttgtgcg tcgaaaacgg ccagccgcgc ttcaaggtcc tgggctgcga tttcgaacgc
    12301 cggaccggcc gggtggtgta cgaaggcgcc ctcgacctcc agggccagga ctacaagacc
    12361 ggttcgctcc ggttcaaggc cgtgcacgcc cgctgtgccg aagtcacctg ggaggccgcc
    12421 aacggcagcc tgtacggggc caccatctgg aaatccccga cgcccgccgc accggatccc
    12481 aaccgcttcg tgaagaccgg cgtggaatac gagaaaccga tcgaattgcg ggagccgacc
    12541 ggggccaacc agctcacgat tggcggaagc ggcacgaacc tttgtgtcac cgtgccggcc
    12601 ggcatgatga acgtgacggc ctcgatgcaa ctgtccacgg gggtcaaggc gctgggggcc
    12661 gtggcggctg atggcgtctt tgtccgcctg gaagacatgc cccgcgagac cgggcgggag
    12721 tttgtgtttt ccttgagccc cggccgtcat ttccttccgt atccggacac gcgggggctc
    12781 ccggccgacg cgcgcgcggt ggatgtgaag gtattcgaca acgccaccaa ggccctcctg
    12841 cgccgtcacc tggcgccgct ggcccggtcg gagacggcct cgccgctgcg gaaggacgag
    12901 gcgccgggcc ccgtcctttc cgagttccgg tatggttcca gcgcctggcc ggtccggttg
    12961 caggacggcg tgctggaggt gcggggcaac gggcgttggg agtggggcga acaatggaaa
    13021 ctggtcgacc ggtatgtgaa agagttcaac ggcgtcggca ccgaccaggg gccggccctg
    13081 gcgcacacga aggacggcca tttctgggac gtggtgctgc tgaactacca gaacggcgac
    13141 cgggtgcccg tcaactccta cagcgagaag gtcgagattt attacggcgg ccagtacggg
    13201 ttctcatatc gcgtgggcgg ccacagcttc gacttcagcc acggcatcct caaggaaatc
    13261 gatccggcca cggggaactt cctgcggccg tgaagcggag cgagcggcat catgaaaaca
    13321 aacctgtggg tgacggccgg gatggcatgg gccatgacct cctgttccgg ggagaagggc
    13381 cccttccgtg aaatccgcgc gtgggactcc agggaaatgc cgtacgccat ggtcttctgc
    13441 gcggcggcca ccaaccagga cgaggccgct gtccggcgcg aggccgaccg gatcgcggcg
    13501 aacaagctgc ggcagtctga acgcgtggtc gtggtcatcc tgtatgacgc ggatcgcgcc
    13561 agggatgtct cctcggagaa ggcgatgcgg aacatgatga gcggcgccgg cgccggggtg
    13621 aatgccttgc tgtacggggg gcggttcgag ggcggaggct tcctgtccgc ggcccgggag
    13681 gggacgacgc ccgcgtggac gtttgttcca ttccgggaca ccggggcgtc ggcccgcgac
    13741 aaggcttttt cccggtagac gggcggaaag gagacccatg aaaacaagga cgttcctggg
    13801 tgggctgggc ctggcgatcg ccacgtgtct cgccgcgggt tccgatgacg ccggcccccg
    13861 catcgtgccg gcggacctgg acgccgccct ggcgtgggct tccgacacgg cggcgtggga
    13921 agggctccgc ggcgcgcgct acgtcaccgc catctctctc aaggcggggg ccgccgaggg
    13981 gatgaccgcc ctcgtaagaa aggagctggc tccgcccggg ccgccggatg gtcccgagcc
    14041 gcaggagacg gccggcagca tcgagtggat caagtacgac gtttcgctcc tcgtgcacgt
    14101 ttcggcggaa tccgccgacg cggcgctgga cggactgctc gcaggcgacc gccggccatt
    14161 tgccgccggc gaagagggct atgtcgtcgt cggcgatggg gtgctgtttc ccgagggcgg
    14221 ggaggccgtc agccagggcc accggaccgc ggcgttccgc gcggggcggt acaacgcagt
    14281 catccagcag gactggcagg gccgccggga actgcccgag ggcccggacc cggtccgctc
    14341 cctcctggac gaggacctgc ggtcgctggc ccaagccctg gcggaacgcg ccgcggccgc
    14401 ttccgaatcc gccgcgccgg agtacagcgt attgttcgac aagctccagg acctcgaggc
    14461 ccggggggac cgtctcgggg cgctgcgcgt gatcccgtcc gtattcgagt gcgatattcc
    14521 cgtcgacgcc ttccaggaaa cgctggccgc cacgcgggca aggtt
//
LOCUS       DAOOTK010000086        14379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2840_length_14379_cov_6.436959, whole genome shotgun sequence.
ACCESSION   DAOOTK010000086 DAOOTK010000000
VERSION     DAOOTK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_14379_cov_6.436959"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK634_09860"
     CDS             <1..624
                     /locus_tag="PK634_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW98081.1"
                     /translation="AFTATPDIVLRVQQRLFDPFFSTRFVGRGLGLSATLGIVHSHGG
                     TIHVQSRLGEGATFRILLPPAAAQPAVLAPARPPAWRGHGTVLLVDDEAPIRQVARMM
                     LEKIGFQVLTASDGREALDIFRSQGDTLACVLLDLTMPGMKGNEVLDELRRLRPGVRV
                     VVSSGFDRKEVLARLANAEEVLFIQKPYTQAKLAEVLSSVLGTGSGT"
     gene            complement(650..1969)
                     /gene="gltX"
                     /locus_tag="PK634_09865"
     CDS             complement(650..1969)
                     /gene="gltX"
                     /locus_tag="PK634_09865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOW98082.1"
                     /translation="MKIRTRFAPSPTGHVHIGNIRAAIYGWLFARHEGGNFLLRIEDT
                     DRERSTPEAVRMVLDAMNWLGLTFDEEPLYQSTRTPAHLAAAEQLLSKGLAYKEDKGG
                     TGMGEAIIFRMPGTDMEFRDEVKGVLRKKAEDMKDFVIVRSNGTPVFHLANIVDDIHM
                     GITHVIRGDDHVENTYRHLALFRALGAEPPKYAHLPMIVNAQGKPYSKRDGAAYVGEF
                     RDKGYLPGALFNYLALLGWSPGDDREIMSREELVKAFTLDRVRSAPSQMDIKKLDWMN
                     GAYMRALPKSELEQGCCSILEQKGLWPVTGGMEYLARVVETMGERLHFFADIADHAAY
                     FFTEDYAFDEKAVSKRLKKEGALESLRTLAGRFAALEPFTAATSEQALRAMAEQRGEG
                     AGALVHPVRVAVSGTSEGPSLFHMLEVLGKDRVLSRIRRALDRFASA"
     gene            2089..3468
                     /gene="mnmE"
                     /locus_tag="PK634_09870"
     CDS             2089..3468
                     /gene="mnmE"
                     /locus_tag="PK634_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOW98083.1"
                     /translation="MSGEDTIAAIATAHGEGGISIVRLSGARSLALAERVIRCPKPPL
                     AERPTNTIVHGYVTDQGRDVDEVLILIMRAPHSYTGEDVVEIQGHGGHVSARRILRCV
                     LQAGARSAEPGEFTRRAFLNGRLDLVQAEAVLDLVRSSSDRAADMALQQLEGRLSDAF
                     NSIYDGLMAAAADLAAALDFPEEDEHPQLIDRPLALLHKVSDKIDQLAATWNEGRVIR
                     NGALVVIFGGANVGKSTLLNTLLEYDRAIVSPIPGTTRDFIEEPFCLNGIPIRLVDTA
                     GIRHTESELEREGIRRAEDVIAKADLHLYVMDASRPLSEQDVANLTIRKPGKCVVVLN
                     KSDLGVQITPEDLQGYDAAVTSLIEKKGLCDVRSLMDKKLIAGMKSGPADTDTPVAIS
                     ERHHDLLVKAQADVKDALAVLNEKHDEAPFLASEKLQSAAESIALIIGRSYRESLVDE
                     IFSRFCIGK"
     gene            3489..5390
                     /locus_tag="PK634_09875"
     CDS             3489..5390
                     /locus_tag="PK634_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW98084.1"
                     /translation="MQSNNSFEVAVVGAGHAGCEAALIAARMGLDTVLVTMDRNAIAR
                     MSCNPSIGGMAKSHIVADLDALGGEMARNTDYTGIQFRTLNTRKGPAVRATRVQCDKH
                     AYSARMAAVIAGTPKLQVVEEAVTDVVIRNGKAIGVNCRSGRVIDSKTVVLCPGTFLN
                     GRIFIGKVSMPGGRAGEEPSNELGNFLASVGFRTGRLKTGTPPRLHRDSLDYSAMREQ
                     PGENPPPFLSRMAISEMFHVEQTPSSHGCGSEVFHVEQQANSLLKWRLGADQMPCYLT
                     HTSQETASIVTGNISESSLYGGLITGTGARYCPSIEDKFVKFPANESHHIFVEPEGRN
                     TVEMYPNGASNSLPESIQLEMIRSIPGLRRAEIVRPGYAIEYDFIDPTQLKPTLETKQ
                     IEGLFLAGQLNGTTGYEEAAGQGLVAGINAAFKVRQEPEFILSRDESYIGVLVDDLVT
                     KGTNEPYRMFTSRAEHRLILRQDNGRFRLLSHSERVGCVSREWIKAVHEDSTIIATEI
                     HRLKCIFSDGVSLAQRLRRSGVGYRDLPAPEIKLGDELARQVEIEVKYEGYIERERKS
                     IDQRRRQEDARIPASIDYDGIRALRFESREKLKKIKPHSLGQAARISGVNPADIAILA
                     IHMSRMNKS"
     gene            5428..5685
                     /locus_tag="PK634_09880"
     CDS             5428..5685
                     /locus_tag="PK634_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOW98085.1"
                     /translation="MIGGAAGIEDEAVDFLEKTTPLARNEWICWIEDAKKEQTRLARI
                     QRVRADLKKGKRRPCCWPGCPHRGGNRMTPADSYFSDMGLQ"
     gene            5884..6495
                     /locus_tag="PK634_09885"
     CDS             5884..6495
                     /locus_tag="PK634_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW98086.1"
                     /translation="MNSDKVSSQSDPAQVADEKDLIRRAQQGDVDAYDVLVRRFQGKI
                     YALVYNMTSNKEDAEDRVQDVFIKAFHSLRHFKGESSFYTWLYRIAINRTINFLKTRK
                     NRITLSLSDMDSGAERDPAYVELRARESPVRDVSLIELQDKLNKALQTLSEKHRVVVV
                     MHDIQGVPHEEIARIMGCSQGTVRSRLFYARQQLQKELKDFAP"
     gene            6492..7037
                     /locus_tag="PK634_09890"
     CDS             6492..7037
                     /locus_tag="PK634_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98087.1"
                     /translation="MNCRHARNIIGWCDGREWPEDVSRHVRECPDCRAFSASMSGIRG
                     LVALKRHETPEAGFESRCAGKIRLGLTAPVAPESIRDEASVWNPAFFLRIAAAAALVL
                     MVGLHVQSVRLTPLASAPGAAQPVAPADQFLAPARFALPSQPLPAVRSGAPVMMLAQS
                     NNGPNGVQYGPLPSRVANWEY"
     gene            7131..8291
                     /locus_tag="PK634_09895"
     CDS             7131..8291
                     /locus_tag="PK634_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOW98088.1"
                     /translation="MPEQDRSAQKAQSSPNSPGEKHASSSFVDMVVSGAAAKKSESRW
                     MQPIDCEVITNCVFDAILVSNHRGHLLNCNSRACELLGYPMEKIERVRLSALLSGIKE
                     SELAGFCQNLQSDKRYVLVEAYCIRADGSRFPAEIVVSMHQRSPPQLCFSLRDITQRY
                     KVEARLRTEHHALQNARDGIVITNEEALIEYANPAFAAMWGYAKTDELAGKNLRPMLA
                     PVSKEAPPEQQPLLWWSLIVDHESWSEDLLAVRKDGSTFHVLANAACNRDAEGRVVGM
                     VLSFTDLTDRNRAEEALRQAERQRAMLASVGAACHHLGQPATVLSTNIELVKRLVDGA
                     KGPELKEILEQSSRAAEQISGILYKLNTVTEFKTVPYLEDDPASPENTILDI"
     gene            8333..8533
                     /locus_tag="PK634_09900"
     CDS             8333..8533
                     /locus_tag="PK634_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98089.1"
                     /translation="MKTFYWAVRVLGYIAGATGMVLFLLGRQSANPRGPMFVAGASLL
                     IVSFAAFFVSYALYIFQRLSRR"
     gene            complement(8540..9190)
                     /locus_tag="PK634_09905"
     CDS             complement(8540..9190)
                     /locus_tag="PK634_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOW98090.1"
                     /translation="MGAYFILFWTAFVIGLSGAMMPGPVLTATISETLKRGFRAGPLI
                     VAGHAILEILVLAAVVAGLGHWITQPAVKRMLGLLGGGLLVVMGAHMAWTAEAAVRVA
                     LETRPDPKAAVRGPMLAGILTSLSNPYWAIWWATIGLNLASLALKNGPSGLAAFYSGH
                     IFSDLAWYSVVAAAVASGRRLCPPPVYRALIIACGLALVGLGGWFFFPAALQPPPA"
     gene            complement(9194..9877)
                     /locus_tag="PK634_09910"
     CDS             complement(9194..9877)
                     /locus_tag="PK634_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOW98091.1"
                     /translation="MSGPSQSGVIFDWDGVVVDSAELHAASWELMAEARGTVLPGGLR
                     PRIGSLGVKTEYVISDMLHWAAAPEDVRRIAEEKEAVYLRLVRERGQPPQPGIRRLLE
                     GLSGHSIPCAVGSSALRRNIEDCIDALGFRRFFKDIVTGDEVRHGKPAPDIFLEAARR
                     IGRRPADCVVFEDAPAGVAAARAAGMRVIGLLTTNPAESLKQADRLIRTFEDLASPDP
                     LAWFEPAAV"
     gene            complement(9874..10716)
                     /gene="tsf"
                     /locus_tag="PK634_09915"
     CDS             complement(9874..10716)
                     /gene="tsf"
                     /locus_tag="PK634_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOW98092.1"
                     /translation="MVEISATLVKELREETNVGMMECKKALVEAGGNKQEAIRILRER
                     GLAIAGKKASRTAKEGQIASEIRQGGKLGAMVEVNCETDFVARNESFQAFLRSVAEKA
                     LMVQGDLAEAMKDILTAKITEIGENMLIRRSVRFEVKQPGIVASYIHLGGKVGVLVEV
                     GCGNEATEKNEAFRELVKDITLHIAACQPHYLARQDVPAEVIKAESDIYAKQVQNKPP
                     QIIQKIVTGKLEKFYDQVCLVEQGFVKDPDISMTDLLAAKGKEIGDTLQIRRFARYQV
                     GEQI"
     gene            complement(10745..11692)
                     /gene="rpsB"
                     /locus_tag="PK634_09920"
     CDS             complement(10745..11692)
                     /gene="rpsB"
                     /locus_tag="PK634_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOW98093.1"
                     /translation="MVAATGVVRSDVNVQDLLEAGLHFGHQTKRWNPKMKRFIFGERN
                     GIYILDLAKTVPLLMEARQFIYDTVIRGRSVLFVGTKKQAQDPLKEMAEKFKQPFVVN
                     RWLGGTLTNLLTIRKSVGRMTEIEKMEQDGTLNKMPKKEVARIKRELEKLHYNLNGIR
                     EMQQLPGAIFVVDICREAIAVAEAVRLNIPVVALVDTNVDPDPISYPIPGNDDAIRAI
                     RLVVDLVGSTIQRAINEAAKLAAEETRKKAIAEAEVAARAKAAEEERKARERLAKKAK
                     ADIAAAKAKAEAEAGGAAAPAAPESAPAAEPPAGDAPTA"
     gene            complement(11876..12454)
                     /locus_tag="PK634_09925"
     CDS             complement(11876..12454)
                     /locus_tag="PK634_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HOW98094.1"
                     /translation="MTALSWAAGGESVPEAASSPEPARRTSLAWMTAGFMIVSFPVIA
                     AHRHNPDWRDPDHDFEDGFTHPPVWDKDDPVFDYVLHPLVGAEYYLVARNRGWDMWGS
                     LAYSAALSAFYEYIPENMVQQPSAVDLLVTPLAGAVLGEARHRLKEWIRGDPARVAAP
                     RFWISLLDPLDISLGGYPDGTARLYFNWQHAF"
     gene            12677..14158
                     /locus_tag="PK634_09930"
     CDS             12677..14158
                     /locus_tag="PK634_09930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW98095.1"
                     /translation="MKHPRRLAIAHFHLRPGGVTRVIGHALAALSRSGVKVVVLAGPS
                     EHRDIPADACVREVEGLGYAEDLAGSAPAAEGLAERLKEAAREALGGLPDLWHFHNHS
                     LGKSAAVPLAIERLAAEGQRLLLQIHDFPEDGRSANYRSLLEHVGERRAALLGRRLYP
                     QAAHIHYATLNTRDLAFLRAAGVAAERLHFLPNPIALRDEARESRPAVLAPKTGLYIY
                     PARAIRRKNIGEFLLWAAVGKKTDRYAVTLAPTSAADLAIYARWRAFARELDLPVEFE
                     AGHDGTVSFPQLLARATAVVTTSVAEGFGLAFLEPWLAGRPLLGRDLPEMTGDFKNEG
                     LDLSGLYARLLVPLDWVGADALRARIEESWPALLAAYGRAPRRGDVEKAYAAACEGER
                     VDFGRLDEGLQETVIRRLKTGRKTLAALSPARLAPEREQAAVVTKNRQLTRQLFNLDQ
                     YGQRLAEIYGHVLESEPAAADPMDPETLLDLFLAPDRFCLLRT"
BASE COUNT         2686 a         4470 c         4512 g         2711 t
ORIGIN      
        1 gctttcactg cgaccccgga catcgtcctg cgggtgcagc agcggctgtt cgatccgttc
       61 ttctccaccc ggtttgtcgg ccgcggcctc ggcctttcgg cgacgctcgg catcgtgcac
      121 agccacggcg gcacgattca cgtgcagagc cgcctcggcg agggagcgac attccgcatc
      181 ctgctgccgc cggcggccgc gcagccggcc gtcctggccc cggcgcggcc tccggcgtgg
      241 cgcgggcatg gcaccgtcct gctggtggac gacgaggcgc ccatacgcca ggtcgcccgg
      301 atgatgcttg aaaaaatagg cttccaggtc ctcacggcgt cggacggccg ggaagcgttg
      361 gatatcttcc ggagccaggg cgataccctg gcgtgtgtcc tgttggacct gaccatgccg
      421 ggcatgaaag gcaacgaagt gctggatgaa ctgcggcgcc tgcggcccgg cgtgcgcgtg
      481 gtggtgtcca gcggattcga ccggaaagaa gtgctggccc ggcttgcgaa tgcggaagag
      541 gttctcttta tccagaagcc ttacacgcag gccaagctgg ccgaggtgtt gagttccgtg
      601 ctgggaaccg gttccggaac gtgaatcggt ccaatgccgc cgtgccggcc tacgcggagg
      661 caaaacggtc cagggcgcgg cggatgcgcg acagcacgcg gtccttgccg agcacctcca
      721 gcatgtggaa caggctgggg ccctcggacg tgcccgagac cgccacgcgg acgggatgca
      781 ccagtgcgcc ggccccctcg ccccgctgct ccgccatcgc gcgcagcgcc tgctcggagg
      841 tggcggccgt gaacggctcc aacgcggcaa atcgtccagc cagggtgcgg aggctctcca
      901 gggcgccttc ctttttcaag cgcttgctca ccgccttctc gtcgaaggca tagtcctcgg
      961 tgaagaaata ggcggcatga tcggctatgt cggcgaagaa atggagcctc tcgcccattg
     1021 tctcgacgac gcgcgccagg tattccatgc ctcccgtcac aggccataat cctttttgct
     1081 ccaatataga acaacacccc tgctccaatt ccgacttcgg cagcgcgcgc atgtaggcgc
     1141 cgttcatcca gtcgagtttc ttgatgtcca tctgcgacgg cgccgagcgg acgcggtcca
     1201 gcgtgaaggc cttgaccagt tcctcgcggc tcatgatctc ccggtcgtcg ccgggcgacc
     1261 agcccagcag cgccaggtag ttgaacaggg ccccgggaag atagcctttg tcgcgaaact
     1321 cgccgacgta cgccgcgccg tcgcgcttgg agtacggctt gccctgcgcg ttgacgatca
     1381 tcggcaggtg cgcatacttc ggcggctccg cgcccagcgc gcggaagagc gccaggtgcc
     1441 ggtacgtgtt ttccacgtgg tcgtcgccgc ggataacgtg cgtgatgccc atgtgaatgt
     1501 cgtccacgat gttcgccagg tggaagaccg gcgtcccgtt ggagcggacg atcacgaaat
     1561 ccttcatgtc ctcggccttc ttccgcagca cgcccttgac ctcgtcgcgg aactccatgt
     1621 ccgtgccggg catccggaaa atgatcgctt cgcccatgcc cgtgccgccc ttgtcctcct
     1681 tgtacgccag gcccttggac agcagctgct cggcggccgc caggtgcgcg ggcgtgcggg
     1741 tcgactggta cagcggttcc tcgtcgaagg tcagccccag ccagttcatc gcgtccagca
     1801 ccatgcggac ggcctccggc gtggagcgct cccggtcggt gtcctcgatg cgcagcagga
     1861 aattcccgcc ctcgtgccgc gcgaaaagcc agccgtagat cgcggcgcgg atgttgccga
     1921 tgtggacatg ccccgtgggg ctgggcgcaa aacgggttcg aattttcatc atgacgcctt
     1981 tccgagccgt agcttgtaca cggtgcgcgc cgggcaacgc aacggaaaac gggcccgttg
     2041 catgccgcgc cctatgcgat ataatgccgc gattccagcg gcgttcccat gagcggagaa
     2101 gacaccattg cggcgattgc caccgcgcac ggcgagggcg gcatttctat tgtccggctg
     2161 tccggcgccc gcagcctcgc cttggccgag cgcgtgatcc gttgccccaa gccgccactg
     2221 gccgagcgtc caacaaacac gatcgttcat ggctatgtca ccgaccaggg gcgggacgtc
     2281 gacgaagtgc tgatcctcat catgcgcgcg ccgcattcct acaccgggga agatgtggtc
     2341 gaaatccagg gccatggcgg acacgtttcc gcgcgccgga tccttcgatg cgtgctgcag
     2401 gccggggcca ggtccgctga accgggcgaa tttacgcggc gcgcattttt aaacggcagg
     2461 ctagacctcg ttcaggcgga ggcggtcctg gacctcgttc gatccagttc cgaccgggcc
     2521 gccgacatgg cccttcagca actcgagggg cgattaagcg atgcattcaa ctccatctac
     2581 gatggcttga tggcggctgc cgcggacctg gcggcagcct tggattttcc tgaagaggac
     2641 gagcacccgc aacttatcga ccgccctctg gctctgctgc ataaagttag cgacaaaata
     2701 gaccaactcg ccgccacgtg gaatgaaggc agagtcatac gaaacggtgc gctggttgtt
     2761 atttttggtg gggccaacgt tggcaaatct accttattaa atacattact tgaatatgat
     2821 agagcaatag tttctcctat tcctggaaca acaagggact ttatcgaaga gccattttgt
     2881 ctcaatggaa taccgataag gctggttgat acagctggga ttcgtcatac cgaaagcgag
     2941 ctggagcgtg aggggatacg tcgcgcggag gacgttattg cgaaggcaga cttgcatctt
     3001 tacgtgatgg atgcatcccg gccgctttcg gaacaggatg tggctaatct gacaatcagg
     3061 aagcctggta agtgtgtcgt tgtcttgaac aagtcggacc tgggtgtgca gattacgccg
     3121 gaggatctgc agggttacga tgccgctgta acatcgctga tagagaagaa ggggttgtgc
     3181 gatgtgcgca gcttgatgga taaaaaactg atagccggca tgaagagcgg tcccgctgat
     3241 acggatacgc ctgtcgcgat atcggagcgc catcatgatc ttctcgtgaa ggcgcaggct
     3301 gacgtaaaag atgccttggc agtccttaat gaaaaacacg acgaggcgcc attcctggca
     3361 tcagagaagt tgcaatccgc cgcagaatca atcgccctga taatcggtcg ctcatacaga
     3421 gagagccttg tagacgagat cttttcaaga ttctgcatcg gtaaataatt attatgcaag
     3481 cagttgcgat gcaatccaac aattcattcg aggtcgctgt tgtcggggct ggtcatgccg
     3541 gatgtgaggc tgcactgatt gccgcgcgca tgggacttga cacggttctt gtcacaatgg
     3601 accggaatgc gatcgcgagg atgtcgtgca atccatccat cggaggaatg gcgaaatccc
     3661 atattgtggc cgatctcgat gcgcttggcg gcgagatggc tcgcaacacg gattacaccg
     3721 gcatccagtt tcgcactctc aatacgcgga aaggtccggc tgtgcgcgca acgcgtgttc
     3781 agtgcgacaa gcatgcctac tcggccagaa tggccgctgt catagcagga acgcctaaac
     3841 tgcaagttgt cgaagaggcc gttacagatg tagtgattcg gaatggcaag gctataggag
     3901 tcaattgtcg cagcggaaga gtcatagaca gcaagacagt tgtcctgtgt ccaggcacat
     3961 tcttgaacgg gaggatattc ataggcaaag tcagcatgcc aggtggacgc gcgggcgagg
     4021 agccttcgaa tgagttgggc aattttctag cctctgtcgg attcaggact gggaggctga
     4081 aaacgggcac gcctccaagg ctgcataggg atagtttgga ttactcggct atgcgagagc
     4141 agccgggaga gaatccgcct ccattcctgt ccaggatggc tatctctgaa atgttccacg
     4201 tggaacaaac tccaagctcg catggctgcg ggtcagaagt gttccacgtg gaacaacagg
     4261 caaattcact tctgaaatgg cgacttggcg ctgatcagat gccttgctat ctcactcata
     4321 catcgcaaga aacagcgtca attgtgacag gaaacatatc tgaaagctct ctatacggag
     4381 gcctgataac tggaacgggc gcaaggtact gtccatccat cgaagacaag ttcgtgaagt
     4441 ttccagcgaa tgagtcacac cacatctttg ttgagccgga aggcaggaat acagtcgaga
     4501 tgtacccgaa tggagcatcc aacagtcttc cggagtccat ccagcttgaa atgatacgct
     4561 ccataccagg cctgcggcga gccgagatag tcaggccagg atatgccatc gagtatgact
     4621 tcatagaccc gactcaattg aaaccaacgc ttgaaacgaa gcagatagaa ggactttttc
     4681 tggctggcca gttgaatgga acgaccggat acgaggaggc cgccggacaa ggcctagtgg
     4741 ctggcataaa cgcggctttc aaggtacgcc aggagccgga gttcattctc tcgcgagatg
     4801 aatcatatat aggagttttg gttgacgatc ttgtgacaaa gggcacgaat gaaccatatc
     4861 ggatgttcac ttcgcgggcc gagcaccgcc tgatcttgcg gcaggataac gggcggttta
     4921 ggttactatc tcattcagaa cgagttggat gtgtttcgag ggagtggata aaagctgttc
     4981 atgaagattc aactattatt gcaacagaga tccataggct gaaatgcata ttctctgatg
     5041 gagtttcgct agcgcagcgg cttcggcgtt caggcgtcgg ctatcgggat ctgcctgccc
     5101 cggagatcaa acttggcgat gaactggcca ggcaggtaga aatcgaagtg aagtatgaag
     5161 gttatattga acgcgagagg aagtcgatcg atcaaagacg gcggcaggag gacgctcgaa
     5221 tcccggcgtc gatcgattat gacgggatca gagcgcttcg tttcgaatcg agggagaaac
     5281 ttaaaaagat caagccgcac agccttggcc aagccgcgag aatatcgggt gtgaatcccg
     5341 cagatatcgc catcctggca atacacatga gccgaatgaa taagtcgtaa tatattgccg
     5401 tgcaatgtgt aacaacgttt gcgttgattg attggtgggg cagccggtat cgaagatgag
     5461 gcggtggatt ttttggaaaa aacaaccccg cttgcgcgga acgagtggat ctgctggatt
     5521 gaggacgcca agaaggagca aacaagactt gctcgaattc agagagttcg cgcggatctc
     5581 aagaagggaa aacgccgtcc ttgttgctgg cccggatgtc cccaccgcgg aggcaacaga
     5641 atgacccctg cagactcgta tttttcggat atggggcttc aatgatccga tggaacaaga
     5701 tactttaggc gcgagcggga aaccgtttat tgttccgtga tccccgagca acgcatctgt
     5761 attggattta cgggtgagag agaacacgga ggcggctgct gctccgaagc ggagagcgct
     5821 cgtcgctttt tgcaggctaa accgcggcgc taatgccgct cgcgaatgtc tgatggacag
     5881 cacatgaaca gcgacaaggt cagcagccag tctgatcccg cgcaggtcgc ggacgaaaag
     5941 gacctgatcc ggcgggcgca gcagggcgat gtcgacgcct acgacgtgct ggtccgccgc
     6001 ttccagggca agatctacgc gctggtttat aacatgacca gcaacaagga ggatgcggag
     6061 gatcgcgtcc aggatgtctt tatcaaggcc tttcactcgc tgcgccactt caagggcgaa
     6121 tcgtcctttt acacgtggct gtaccgcata gccatcaacc ggacgatcaa cttcctcaag
     6181 acgcgcaaga atcgcatcac cttgagcctc agcgatatgg actctggcgc cgagcgggat
     6241 ccggcctacg tggagctccg ggcgcgggag tcgccggtcc gcgacgtgtc cctgatcgaa
     6301 ctccaggata aattgaacaa agcgctgcag accctgtcag agaaacatag agtggtggtg
     6361 gtcatgcatg acattcaggg cgtgccgcat gaagagattg ctcggatcat gggttgctcc
     6421 cagggaacgg ttcggtcgcg cctgttctat gcccggcagc agttgcagaa ggaattgaag
     6481 gattttgcgc catgaactgc agacacgcga ggaacatcat cggatggtgt gacggccgcg
     6541 agtggccgga ggacgtgagc cggcacgttc gggaatgccc ggactgccgc gccttttccg
     6601 catccatgag cggcatccgt ggactcgtag cgctgaagcg ccacgagact ccggaggcgg
     6661 gcttcgagag ccgttgcgcc gggaagatcc gccttggcct tacggccccg gtagcgccgg
     6721 aatcgatccg cgacgaggct tcggtgtgga acccggcgtt cttcctgcgc atcgcggcag
     6781 cggcggcgct ggtgctgatg gtgggcctgc atgtccagtc cgtgcgcttg acccctctgg
     6841 cctccgcgcc tggcgcggcc caaccggttg cgcctgccga ccagttcctg gcgcccgccc
     6901 ggtttgccct gccgtctcaa cccctgccgg ccgttcgttc cggcgcgccg gtcatgatgc
     6961 tggcccagtc gaacaatggg ccgaacggcg tccaatacgg cccgctgccc tcgcgcgtgg
     7021 ccaactggga atactgatcc gccagcgaac tgccgttgtc gcaacttctt ttcccccgca
     7081 ggttggtacg gttcctgcat ttaatcaatc gggaataccg gagaccgacc atgccggagc
     7141 aggataggag tgcccagaaa gcgcaatctt cgccgaattc gcccggcgaa aaacacgcct
     7201 cctcatcctt tgtggatatg gtggtctcgg gcgccgccgc gaagaaatca gaatcgcgct
     7261 ggatgcaacc gattgattgc gaagtgatta cgaattgcgt gttcgacgcg attctcgttt
     7321 ccaaccatcg tgggcatctc ttgaactgca actccagggc gtgtgaattg ctgggctatc
     7381 caatggagaa aatcgagcgg gtccgcttgt ccgccttgct ctccggcatc aaggaatcgg
     7441 aactggccgg cttttgtcaa aatctacaaa gcgacaagcg ctacgtgctg gtcgaggcct
     7501 actgtatccg cgcggatgga tcccggttcc ccgcggaaat cgtggtgtcc atgcaccagc
     7561 gctcgccgcc gcaactctgc ttctcgttga gagacatcac gcagcgctac aaggtcgagg
     7621 cccggcttcg tacggagcac cacgccttgc agaatgcccg cgacggtatc gtcattacca
     7681 acgaagaagc gctgatcgag tacgccaatc ccgcgtttgc ggccatgtgg ggatacgcga
     7741 agaccgatga gctggccggg aaaaacctgc gccccatgct ggccccggtt tccaaggagg
     7801 cgccgcccga acagcaaccg ctgctatggt ggtcgctgat cgtcgaccac gaatcgtggt
     7861 ccgaggatct cctcgccgtg cgcaaggacg gatcaacctt ccatgtgctt gccaacgcgg
     7921 cctgcaaccg cgatgccgag gggcgggtgg tcgggatggt gctttcgttc accgacctga
     7981 cggatcgcaa ccgcgccgag gaggcgctgc gccaggcgga gcgccagcgg gccatgctgg
     8041 ccagcgtggg cgccgcctgc catcatctcg gtcagccggc cacggtcctg tcgaccaata
     8101 tcgagttggt caagcggctc gtggacggcg cgaagggccc ggaattgaag gaaatcctgg
     8161 aacaaagcag ccgggcggcg gagcagatat ccggcattct ttacaagctc aacaccgtca
     8221 cggagttcaa gaccgtgcct tatcttgagg acgatccggc ctcgccggaa aacaccatcc
     8281 tcgacatctg atccgtccgc attatgcttc ccgccatgga tgggggcgac gcatgaagac
     8341 cttctactgg gccgtccgcg tgctggggta catcgccggc gcgaccggaa tggtcctgtt
     8401 cctgctgggc cgccagagcg ccaatccgcg ggggccgatg ttcgtcgcgg gcgcctcgct
     8461 cctgatcgtt tccttcgccg cgttcttcgt gtcgtacgcg ctgtatatat tccaacgcct
     8521 ctcgcggcgc tgaaacatgt caggcgggcg gtggctgcaa ggccgccggg aagaaaaacc
     8581 atccgcccag tccgaccagc gccagcccgc acgcgatgat gagcgcgcgg tagaccggcg
     8641 gcgggcacaa gcgccgcccc gaggccacag ccgccgcgac cacgctatac cacgccaggt
     8701 cggagaagat gtgccccgag taaaaggcgg cgaggccgga cggcccgttc ttcagcgcca
     8761 gggaggcaag gttcagcccg atcgtggccc accagatcgc ccagtaggga ttggaaagcg
     8821 acgtcaggat gccggccagc atcgggccgc gcaccgcggc tttcgggtcg ggccgggttt
     8881 cgagcgcgac gcgcaccgcg gcctccgcgg tccacgccat gtgcgcgccc atgacgacca
     8941 gcagcccgcc gcccagcagg cccagcatgc gcttgaccgc gggctgggtg atccagtgac
     9001 cgagccccgc gacgacggcg gccagcacca ggatttccag gatggcgtgt cccgcgacga
     9061 tcagcggccc cgcgcggaag ccgcgcttga gcgtttcgct gatcgtggcc gtgagcacgg
     9121 ggcccggcat catggcgccg gacagtccga tgacgaacgc ggtccagaac aggatgaaat
     9181 aggcgcccat gggctacacg gcggcgggct cgaaccacgc gagcggatcc ggcgaggcca
     9241 ggtcctcgaa cgtgcggatc aggcggtccg cctgcttcag cgactccgcg gggttcgtcg
     9301 tgagcagccc gatcacccgc atgccggccg cccgcgcggc ggccaccccc gcgggcgcat
     9361 cctcaaacac cacgcagtcg gccggacggc ggccgatccg ccgcgccgcc tcgaggaaaa
     9421 tatccggcgc cggcttgccg tgccgcacct cgtcgccggt cacgatgtct ttgaagaacc
     9481 ggcggaagcc cagcgcgtcg atgcagtcct cgatgttccg gcgcagggcg gaagagccga
     9541 cggcgcacgg gatcgagtgg ccggagaggc cctccagcag gcgccggatg ccgggctgcg
     9601 gaggctggcc ccgttcccgc acgaggcgga gatagacggc ctccttctcc tcggcgatcc
     9661 ggcggacgtc ctcgggggcg gcagcccagt gcagcatgtc gctgatgaca tactcggtct
     9721 tcacgcccag gcttccgatg cgcgggcgca ggccgcccgg aaggacggtg ccccgcgcct
     9781 cggccatgag ttcccagctc gcggcgtgga gctccgcgga atccaccacc acgccgtccc
     9841 agtcgaagat cacccccgat tgagacggcc cgctcaaatc tgctcgccca cctggtagcg
     9901 ggcgaaccgc cggatctgca gcgtgtcgcc gatctccttg cccttggcgg ccagcaggtc
     9961 cgtcatggaa atgtcgggat ccttcacgaa cccctgctcc accaggcaca cctggtcgta
    10021 gaacttctcc agcttgcccg tgacgatttt ctggatgatc tgcggcggct tgttctgcac
    10081 ctgcttggcg tagatgtcgg attccgcctt gatcacctcg gccgggacgt cctgccgggc
    10141 caggtaatgc ggctggcagg cggcgatgtg cagcgtgatg tccttgacca gttcgcggaa
    10201 ggcctcgttc ttttccgtgg cctcgttccc gcacccgacc tcgaccagca cgccgacctt
    10261 gccgcccagg tgaatgtagc tggccacgat gccgggctgc ttcacctcga accggacgct
    10321 gcggcggatc agcatgttct cgccgatctc cgtgatcttg gccgtgagga tgtccttcat
    10381 ggcctcggcc aggtcgccct gcaccatcag ggccttctcg gccaccgagc ggaggaacgc
    10441 ctggaacgat tcgttgcgcg cgacgaagtc ggtctcgcag ttcacctcga ccatcgcgcc
    10501 gagcttcccg ccctggcgga tttccgaggc gatctgccct tccttcgcgg tgcgggacgc
    10561 cttcttgccg gcgatcgcca gcccgcgctc gcgcaggatg cggatcgcct cctgcttgtt
    10621 gccgccggcc tcaacgagcg ccttcttgca ctccatcatg ccgacgttcg tttcctcgcg
    10681 cagttccttg accagcgttg cactgatttc gaccatggta agctcctttt ctcgatttct
    10741 ggatttaggc cgtaggggcg tcgccggccg gcggctcggc ggcgggcgcg ctttccggcg
    10801 cggccggggc tgcggccccg cccgcttccg cctcggcctt tgccttggcg gccgcaatgt
    10861 cggccttcgc cttcttcgcc aggcgctcgc gcgccttgcg ctcttcctcc gcggccttcg
    10921 cgcgcgcggc cacctcggcc tcggcgatcg ccttcttgcg ggtttcctcg gcggccagct
    10981 tcgccgcctc gttgatcgcc cgctggatcg tgctgccgac caggtccacg accagccgga
    11041 tcgcccggat ggcgtcgtcg ttgcccggga tcgggtagct gatcgggtcc ggatccacgt
    11101 tggtgtccac cagcgcgacg accgggatgt tcagccggac ggcctccgcg acggcgatcg
    11161 cctcgcggca gatgtccacc acgaaaatgg cgcccggcag ttgctgcatt tcgcggatgc
    11221 cgttcaggtt gtagtgcagc ttctccagct cccgcttgat ccgcgccact tccttcttcg
    11281 gcatcttgtt gagcgtgccg tcctgctcca tcttctcgat ctcggtcatg cggccgacgc
    11341 tcttgcggat ggtcagcagg ttggtcagcg tgccgccgag ccagcggttg acgacgaacg
    11401 gctgcttgaa cttctccgcc atctccttca gcggatcctg ggcctgcttc ttggtgccca
    11461 cgaacagcac cgaacggccc cggatgaccg tgtcgtaaat gaactgccgc gcctccatca
    11521 gcagcggcac ggtcttggcg agatcgagta tatagatccc gttgcgctcc ccgaagatga
    11581 accgcttcat cttcggattc cagcgcttgg tttgatgacc gaaatgcaga cccgcttcga
    11641 gcaggtcctg gacgttgacg tccgaacgga ctacacctgt agcagcaacc acgacttcct
    11701 ccgtatgttg ccccggctgg gccggggaat ccgctttggc tccgggcgga gacccggggc
    11761 actcgcttcc gtcccgcaca cgcaggacta gggcgcggac tataccatcc cgttttcggc
    11821 ctgcaacagt ttttttcgcg cgaaatggag ggcgcgggcg ccggcttggc tcgcatcaga
    11881 aagcgtgctg ccagttgaag tacaggcgcg ccgtcccgtc cgggtagccc ccgagcgaga
    11941 tgtccagcgg gtcgagcagg ctgatccaga accggggcgc ggcgacgcgg gcgggatcgc
    12001 cccggatcca ttccttcagc cggtgccgcg cctcgcccag cacggccccc gccagcggcg
    12061 tgaccagcag gtcgaccgca gaaggctgct ggaccatgtt ctccgggatg tactcgtaaa
    12121 acgcggaaag cgccgcgctg taggccagcg agccccacat gtcccagccg cggttgcgcg
    12181 cgacgaggta gtactccgct ccgaccagcg ggtgcagcac gtagtcgaac accggatcgt
    12241 ccttgtccca caccggtgga tgcgtgaacc cgtcctcgaa atcgtggtcg ggatcgcgcc
    12301 agtccggatt gtgccggtgg gccgcgatga cggggaacga cacgatcatg aagcccgcgg
    12361 tcatccacgc caggctcgtg cgccgggccg gttcggggct ggaggccgct tcgggcacgc
    12421 tctcgccgcc cgccgcccat gagagtgcgg tcaagagcaa aacggtcagc gcgtgccgca
    12481 ccatgggaag acgggtcgag gatgggcagt agtggcttcg gcggcgcacg gggaaaacgt
    12541 aggccaaaag gaagccgtcg tcaatggcgg cggccgtaaa ccggctcgac gccgcgtcat
    12601 tctgctgcta tgagagagca gcaatgaacg ggcagcaaag cgagtcgacg gaacgaccgg
    12661 ccccggagcg tgggccgtga aacatcctcg tcgccttgcg atcgcgcatt ttcacctgcg
    12721 ccccgggggc gtcacccgcg tgatcggaca cgccctggcg gccctctcgc gttcgggcgt
    12781 caaggtcgtc gttctggccg gaccctccga gcaccgcgat attcccgccg atgcctgcgt
    12841 ccgcgaggtg gaggggctgg gatatgccga ggatcttgcc ggttcggccc cggccgcgga
    12901 aggcctggcg gagcggctga aagaggccgc ccgggaggcc cttggtggat tgcccgacct
    12961 ctggcacttt cataatcact cgctgggaaa aagcgccgcc gtcccgctcg ccatcgagcg
    13021 gctcgccgcg gaaggccagc gcctcctgct gcagattcac gattttcccg aagacggccg
    13081 ctcggccaac taccgcagcc tgctggagca cgtcggcgaa cgccgggccg cgctgctcgg
    13141 ccgccggctg tacccccagg cggctcacat tcattacgcc accctcaata cccgcgacct
    13201 tgctttcttg cgcgccgccg gcgtggccgc ggaacgactg catttcctgc ccaaccccat
    13261 cgccttaagg gacgaggccc gggaatcgcg tccggccgtg ctggcgccca aaacgggcct
    13321 ctatatttat cctgctcgcg ccatccgccg gaaaaatatc ggcgagtttc tcctgtgggc
    13381 cgctgtgggt aagaaaacgg atcgctatgc cgtcaccctg gccccgacca gcgccgccga
    13441 tctcgccatc tatgcccggt ggcgggcgtt tgcgcgcgag ctggacttgc ccgtcgagtt
    13501 cgaggccggg cacgacggga cggtctcttt cccgcaactg ctggcccggg cgacggccgt
    13561 cgtgaccacc agcgtggcgg aaggattcgg cctggccttc ctggagccgt ggctggccgg
    13621 tcgcccgctc ctcggccgcg atctccccga aatgaccgga gatttcaaga atgagggatt
    13681 ggatctctcc ggcctgtacg cgcgtctcct ggttcccttg gactgggtgg gcgccgacgc
    13741 cttgcgcgct cgtattgaag aatcctggcc cgcgcttctg gcggcttacg gccgggcccc
    13801 ccgccggggc gatgtggaga aagcctatgc ggccgcctgc gagggcgagc gcgtggattt
    13861 cggccgcttg gacgagggct tgcaggaaac cgtcatccgg cgcctgaaga ccggcagaaa
    13921 aacgctcgcc gcgctctccc ccgcccggtt ggcgcccgag cgcgagcagg ccgccgtcgt
    13981 cacgaagaat cgccaattga cccgtcagct tttcaacctc gaccaatatg ggcagcgcct
    14041 ggctgaaata tacggccatg tgctggaaag cgagccggcc gcggcggatc ccatggatcc
    14101 ggaaacgttg ctcgatctgt ttcttgcccc ggaccgcttc tgtctcttgc ggacctaata
    14161 tcgtttatag cgtgaaacgg cacgatttgg tagggcgcgc tcgccgagcg cgccgcagcg
    14221 gcttgagcca agccgcccta cctgcaaatc ccgggatcgt gcactttcaa gcaggaaatg
    14281 gtagaaaacg cgcgccgggg gatcggtcgc catgtctggg aacgctagcc cgcttattgc
    14341 atgagcgtag agacgctcac gcaataagcg cttctgggc
//
LOCUS       DAOOTK010000087        13745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2982_length_13745_cov_5.954492, whole genome shotgun sequence.
ACCESSION   DAOOTK010000087 DAOOTK010000000
VERSION     DAOOTK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2982_length_13745_cov_5.954492"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..2372)
                     /locus_tag="PK634_09935"
     CDS             complement(198..2372)
                     /locus_tag="PK634_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOW98096.1"
                     /translation="MTIARHDAIMAVTKYRPIEKPLDFSKTPSKELFGSSVFNDKVMK
                     KRLPEKIYKALRKTIDHGERLDIRIAEPVAAAMRDWAIEKGATHYAHVFYPLTGLTAE
                     KHDSFLDPDGKGGVIAAFSAKQLIQGEPDASSFPSGGIRATFEARGYTAWDVTSPAYI
                     LENPNGTTLCIPTAFCSWTGEALDKKTPLLRSMQALNAQARRVLAIFGQNDPGLIYAT
                     AGCEQEYFLIDRNFFFARPDLINAGRTLFGAKPPKGQELEDQYFGAIPERVLACMLET
                     ERELYKLGVPVKTRHNEVAPAQYEIAPVYENANLATDHQYLTMVTLQRVAQKYGMSCL
                     LHEKPFAGINGSGKHLNWSLGTTKQNLLTPGKTPHSNAQFLVFCAAVIRAVDKHADLL
                     RGAVAHAGNDHRLGANEAPPAIISVFLGAQLTDIFEQIARGGAKDSKKPGVLTVGVDT
                     LPPLPKDAGDRNRTSPFAFTGNKFEFRAVGSSQSVANPLIVLNTIVAESLDFIATELE
                     AKTKGNTKKLNDAVQDVLQQIINEHGRIIFNGDNYSEAWHKEAEKRGLPNFRNTVDAL
                     HGMDTDENAELFEKYNVLTKREFKSRNEIYLEKYIKEVNIESRLVLRIAKTMIFAAAV
                     DYQDKLAKTSQALKSLGKDHCTTVLDELNTLLGKLQPALAALESAIHHESKGGLLDHA
                     RHMRDKIIPTMVEVRQISDRIEGIVSDDLWPLPTYQEMLFIK"
     gene            complement(2710..3615)
                     /locus_tag="PK634_09940"
     CDS             complement(2710..3615)
                     /locus_tag="PK634_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOW98097.1"
                     /translation="MKLTLMIMAAGMGSRYGGLKQLDPVGPGGEIIMDYSVFDALRAG
                     FHRVVFVIRRSIEQDFRRAIGSRFEKRLETVYAFQELDSLPAGFAVPAERQKPWGTGH
                     AMLVGRDLVPGPFAVINADDFYGAGAFQLLADYLRSAAARGREYAMVGYTLRDTLSDH
                     GTVTRGICECDDRLYLRRIVETAQLARDGQRAVSIDVQGARHSFTGDEWVSMNAWAFT
                     PTIFDHLDREFRVFLETRRADPKAEFFIPGVVGRLIEEQRARVRILPGASRWFGITYR
                     NDKPFLESALKEMAAQGAYPARLWS"
     gene            complement(3701..4516)
                     /locus_tag="PK634_09945"
     CDS             complement(3701..4516)
                     /locus_tag="PK634_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOW98098.1"
                     /translation="MKFSQPQASLLVPDGRPADQALAGISHLGIGAHPDDAEIIALHG
                     ILACRETRGFGAVICAHGGERVRDVRRREQEEAARMGRYGILAMLDHRSADLKGPAVS
                     ALEADIQALFGAARPETVYTHHPADRHETHVAVSLAVVRALRRLPPDRRPKSLYGVEV
                     WGSLDWLPAEGRIELNVSSGGTLAAELIRVFRSQIEGGKRYDAAAAGRRAANATFADP
                     YAPDRARELCLAMDLTPLLRDPGLDAGRYVARLLDSFRREALDRVRRVSGPGE"
     gene            complement(4581..5834)
                     /locus_tag="PK634_09950"
     CDS             complement(4581..5834)
                     /locus_tag="PK634_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98099.1"
                     /translation="MKATIIGCLALGLTALSSAPVMSEEAMPTDDFGAMVKEFSAARH
                     KLAVELAEQLDLVLPKKAVSFFEAAEAGDWAATSNRFQKLKTPGDSGWSVPSMRNELW
                     ATVHETLGVWEVWVGWKQDSALLRMFYEPILSSMPEGSIYFGGTDCGRFVVTTANALK
                     QPPPVFVLTQNGLADNTYMAHLRAVYGDRLWIPEQEDSNQAFQEYVEDVQAGRYPAGA
                     EVSFEDGRVTVQGVAGVMMINGILCRMIFERNKDEHPFFLEESYVVDWMYPYLEPHGL
                     ILKLNPEPLPELTADAVERDRQFWKDTLARLESQPGWASNPDARKAFSKLRSAIAGVY
                     VYRKKYDEAEAAFRQAIDLYRFSPEAHMRLAKMYEEQDRLEEARDLMVAFMRSDPPEA
                     GEQAGQYLDQLETKVRAGGEAIPVP"
     gene            complement(5855..6964)
                     /gene="rsgA"
                     /locus_tag="PK634_09955"
     CDS             complement(5855..6964)
                     /gene="rsgA"
                     /locus_tag="PK634_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOW98100.1"
                     /translation="MARRVDLDHKTPMELTSLGFDSWFEERVAGLLPPGRCLARVTAV
                     DRDAFLIRDPDGERYAELSGKFRFTARSAGDLPCVGDWVCAQRPESDGPAIIHEVWPR
                     KTLLRRKRPGKVVDFQMIAANIDTAFIVQGCDFDFNVPRLERYLVMARDGRMGAHIIM
                     SKTDLVSPEELERRIEDIRRSGISAPIARLSNATGAGMEAFRAALAPGKTYCLLGSSG
                     VGKTTLINRLLGKEAFETRAVSGTGEGVHTTSRRQLLVLDNGAMLIDTPGMRELGLLG
                     AEDGIDATFHDIADYSTRCRFPDCTHTSEPGCAVLAALQDGELSEERYQGYLKLKKEA
                     EYHNLSYVEKRKKDKTFGRFFKSAMKQMKRSSVKW"
     gene            complement(7332..9749)
                     /locus_tag="PK634_09960"
     CDS             complement(7332..9749)
                     /locus_tag="PK634_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98101.1"
                     /translation="MSTLHLKKTEGDFHNFLVRDTQTRVAAHLQLPVGKSGLRVVVAM
                     PAGKSALCFETTQPAGWTLEGAPLPFADADGQAGVQFTLASASPELLITAASILLNHT
                     LVARFGDDPDRRHENRLKDLVRSGAIGEIESVLGFSLRDLADRGQTVGPVFDAGRNTI
                     TFTRRALDGRHRYRLEFAGGPATDVRVRGDCLRITRKDPPGTAPLRLLVRASTDFALL
                     TPMPFAELVNERGRALAAKDPLFAESIRNFEFLSYREKFLAGSWNFLSYFGRDTLISL
                     RIMWPVLSAQARRAGIQSVADEISAGGIVNVTDEWTDDRAVMDALEGFFHTYGRGDLD
                     RARPLMQTILQGTVPEHPFLDVLDQTYMFPSAAAFWFGEIGDGDLSQWLQEEHLVLGR
                     RESNRVTLLRNWNYLLHAAAPYVAAWQSLRTKYPDLTPAQLIAAYPDEFKAAGSSLIT
                     SVAGAANWRDTYNLPWRFRSEDINVHLLPMVIGAIREMIARIQALGLGDGLLGEAKGN
                     ALDVIREFIEKPDRFAAAAEAWDPDLVRAHFLVRRSACDIRRDLQRYLEGLARGEVTW
                     DDRERGCRERAAILRGRVDGVTVADFLRGDRVPDAIKDGIEFTALLLDTECNPLPLMH
                     CDDVFALLFGKPSMEQVRQIIRPLVLSYPFGLGFMEEDLGMAITNTVYAPRDIPALQD
                     NGKNAWVKFGPDEYHGRAAWPWTLFALIIGIDQLVQRSIDADGTLRDGLTREDVELFR
                     KILLKTKAALVKLGPLATSEVFKYAPARAGEGIWQAEPLGISTPIQLWSAAPANLVID
                     EALERLP"
     gene            complement(9880..10326)
                     /locus_tag="PK634_09965"
     CDS             complement(9880..10326)
                     /locus_tag="PK634_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98102.1"
                     /translation="MEVQPDFKELLALFNEQKIEYIIVGAYALAYHGAPRFTGDIDLY
                     VRPTKNNAARIIAALSSFGFGSLGLKEEDFQKPEQVIQLGVPPVRIDLITSITGVTWE
                     EANAGKVSGKYGDVDVLYLGRDQYAQNKRATGRKKDLADLEALGED"
     gene            complement(10916..12823)
                     /locus_tag="PK634_09970"
     CDS             complement(10916..12823)
                     /locus_tag="PK634_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HOW98103.1"
                     /translation="MKKPSRWCGYQKFQVIQFISVLVLLLPVSGSGVELGTREQTILP
                     RVVAPETPPVAPSNAAAYLTSPYGQWDWGPGTNEGRLLTLMPEGYTGTNHAARLLSFF
                     SLADVHMTDKESPAQVPCLGWSADFGDAGIAQLNPSAYTPVILATTHRLDAAVKTINA
                     LHRLASFDFGIVLGDMCNSGQYNELRWFVEVMDGQQITPSSGAHDGADTIDYQMPYQA
                     AGLDRSIPWYAVIGNHDQLWMGIGYPSEKIQQAMVGSNVLNISPNGPLIPPGAEGIGM
                     YVGVVDGTTPYGTVIKWGATNLFETPPTVAADPDRRMLSTPLNSPTNFVNAFFDSTSL
                     PQGHGFTPGVTGSLAACYSFEPATNLPIKVIVLDNTCKADVPGQSALFYGGGWVDAAR
                     FSWLTNELQMGQNEDQLMILACHIPILPQTSATNTSRSSTFQDPASESNLVATLHNYP
                     NLLLVMAGHRHQNVVTAFPSPDPAQPERGFWEVETVSLRDFPQQLCAWEILRNSDNSI
                     SILTTSVDPVLEEDSPAWDSRTYGIAAERIFGRIALDDTSSRTYNVELVKQLTPAMQA
                     KIAGIGEPLGHPMHIESADSGMAIDHLGQLQAADAMVEPITWDDVSVATSSSHSVSPQ
                     RGQLMNRAAEP"
     gene            complement(12914..13180)
                     /locus_tag="PK634_09975"
     CDS             complement(12914..13180)
                     /locus_tag="PK634_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOW98104.1"
                     /translation="MKNRSTEMKSRTDWKRLNSMKDEDIDASGVPRIDPSIFKKMVIR
                     MPKPKELVSIRIDPDVLGWFRQQGRKYQTRMNAVLRSYVEAHTR"
     gene            13312..13518
                     /locus_tag="PK634_09980"
                     /pseudo
     CDS             13312..13518
                     /locus_tag="PK634_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(13499..>13745)
                     /locus_tag="PK634_09985"
     CDS             complement(13499..>13745)
                     /locus_tag="PK634_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOW98105.1"
                     /translation="PILAVIALHAFTVAYSNFMFALLICQDERMWTLMVWLYQLQARS
                     GQAVIYASLLVAALPTLLLFLFCQKIILRGIVIPVER"
BASE COUNT         2377 a         4305 c         4416 g         2647 t
ORIGIN      
        1 gcgttggaag ccagcgcgcc caccgtgaca tccacctggg ccagggcaac aaccagcgat
       61 ttcatattac aatattgtag ccctgttgga tgaaaatgac aaacggaatc ggcggcgggc
      121 gggatgattt tcatgttcgg caacgaaaca cccgtccgcg ggggacgggt gtttcgcggg
      181 cctgcgcaag gaggccgtta cttgatgaac agcatctcct ggtaggtcgg tagcggccac
      241 aggtcgtcgg acacaatacc ctcgatccgg tccgatatct gccggacctc gaccatcgtg
      301 ggaatgatct tgtcccgcat gtgccgggca tgatcaagca acccgccctt ggactcgtgg
      361 tggatggccg actccagcgc ggcgagggcc ggctggagct tcccgagcag ggtgttcaac
      421 tcgtccagca cggtcgtgca gtggtccttg ccgagcgact tgagggcctg cgaggtcttc
      481 gccagcttgt cctgatagtc cacggccgcg gcgaagatca tcgtcttggc gatccgcagc
      541 acgagccggc tctcgatatt gacctccttg atgtacttct ccaggtagat ctcgttcctg
      601 ctcttgaact cccgcttcgt caggacgttg tacttctcga acagctccgc gttctcgtcc
      661 gtgtccatgc cgtggagggc gtccacggtg ttgcggaagt tgggcaggcc gcgcttctcg
      721 gcctccttgt gccacgcctc gctgtagttg tccccgttga agatgatccg gccgtgctcg
      781 ttgatgatct gctgcagcac gtcctgcacg gcgtcgttca gcttcttcgt gttgcccttg
      841 gtcttcgcct cgagctcggt cgcgatgaag tcgagcgact cggcgacgat cgtgttgagg
      901 acgatcagcg ggttggcgac ggactggctg gagccgaccg cgcggaactc gaacttgttg
      961 ccggtgaagg cgaacgggct ggtccggttg cggtcgcccg cgtccttcgg caggggcggc
     1021 agggtgtcca caccgaccgt cagcacgccc ggcttcttgg aatccttcgc gccgcccctg
     1081 gcgatttgct cgaagatgtc cgtgagctgg gcgccgagga agacggagat gatggcgggc
     1141 ggcgcctcgt tggcgccgag ccggtgatcg ttcccggcgt gggccaccgc cccgcgcagc
     1201 aggtccgcgt gcttgtccac ggcgcggatc accgcggcgc agaacacgag gaactgggcg
     1261 ttgctgtgcg gcgtcttgcc gggcgtcagc aggttttgct tcgtggtccc cagcgaccag
     1321 ttcaggtgct tgccggaccc gttgatgccc gcgaagggct tctcatggag caggcaggac
     1381 atcccgtact tctgggccac ccgctgcagg gtgaccatcg tcaggtactg gtgatcggtc
     1441 gcgaggttcg cgttctcgta gaccggggcg atctcgtact gcgccggcgc gacctcgttg
     1501 tggcgggtct tcaccgggac gccgagcttg tacagttcgc gctcggtctc gagcatgcag
     1561 gccagcacgc gctccgggat ggcgccgaag tactggtcct ccaactcctg gcccttcggg
     1621 ggcttggcgc cgaagagcgt ccggccggcg ttgatcaggt cgggccgcgc gaagaagaag
     1681 ttgcggtcga tcaggaagta ctcctgctcg caacccgcgg tggcgtagat caggccgggg
     1741 tcattctggc cgaagatcgc gagcacccgc cgggcctgcg cgttcagggc ctgcatcgag
     1801 cggagcaggg gggtcttctt gtccagcgcc tcgcccgtcc acgagcagaa ggccgttggg
     1861 atgcagaggg ttgtgccgtt cgggttctcc aggatgtagg ccgggctggt cacgtcccaa
     1921 gccgtgtacc cgcgggcctc gaaggtcgcg cggatgccgc ccgaggggaa actggaggcg
     1981 tcgggctcgc cctggatcag ctgcttggcc gaaaaggccg cgatcacgcc gcccttgccg
     2041 tccgggtcga ggaagctgtc gtgcttctcg gccgtcagcc ccgtcagcgg atagaagacg
     2101 tgcgcgtagt gcgtcgcgcc cttctcaatg gcccagtcgc gcatggcggc ggccaccggc
     2161 tcggcgatgc ggatgtccag ccgctccccg tgatcgatgg tcttgcgcag cgccttgtat
     2221 atcttctcgg gcaggcgctt cttcatgacc ttgtcgttga aaacgctcga accgaacagc
     2281 tccttggacg gcgtctttga aaaatccagc ggcttttcaa tgggtctgta cttcgtcacg
     2341 gccataatcg cgtcatgtcg ggcaatcgtc atcgcgttct cctcgtcttc gttcgtcttt
     2401 tttgaagcag gttcccgtac gcttttaccg actcctcgtt caaaagcaac actgccaagc
     2461 gggaccatcg cctgctttac ctgcgccttg cattagcaat agccatgcca atccgccgcg
     2521 cgcagccgcg tcataatgtg taacaatctt acctgcaata taatatgttt gtgtcggata
     2581 aaatgcccgc cacccattgc gatacactat tgtagtagct attgccttga tattacatga
     2641 atgtgcatcg atggcatatc cccatgcgag gttgtgttga cgcgttgcct ggggacagcg
     2701 cggcgcaggt caggaccaga ggcgcgcggg ataggcgccc tgcgcggcca tttcttttag
     2761 cgccgattcg aggaagggct tgtcgttgcg gtaggtgatg ccgaaccagc ggctggcgcc
     2821 gggcagtatc cggacccggg cccgctgctc ctcgatcagg cggccgacga cgccggggat
     2881 gaagaactcc gctttcggat cggcgcggcg ggtttccagg aagacgcgga actcgcggtc
     2941 gaggtggtcg aagatcgtcg gcgtgaaggc ccaggcattc atggaaaccc actcgtcgcc
     3001 ggtgaacgaa tgccgggctc cctggacatc gatggacacg gcccgctgcc cgtcccgggc
     3061 cagttgggcg gtttcgacaa tccgccgcag gtacagccgg tcgtcgcatt cgcagatgcc
     3121 gcgggtcacc gtgccgtgat cggagagcgt gtcccggagc gtgtagccga ccatcgcgta
     3181 ttcccggcct ctggccgccg ccgagcgcag gtaatccgcc agcagttgga aggcccccgc
     3241 gccgtagaaa tcgtccgcgt tgatcacggc gaacggcccc gggacgaggt cgcgtccgac
     3301 gagcatcgcg tggcctgtgc cccacggttt ctgccgctcc gccggcacgg cgaagcccgc
     3361 gggcagggag tcgagctcct ggaaggcgta gacggtctcc agccgtttct cgaaccggct
     3421 gccgatggcc ctgcggaaat cctgttcgat cgagcggcgg atgacgaaga ccacgcggtg
     3481 aaagcccgcg cgcagggcgt cgaacacgga gtagtccatg atgatctcgc cgccgggccc
     3541 gaccgggtcc agttgcttga gcccgccgta ccggcttccc atgcccgccg ccatgatcat
     3601 cagggtcagt ttcatgcgtt gctccatgct ctcgggccgg cccggggctc cgacgcataa
     3661 cgccatcagc ctagcagggc gtcccccctt gattccagcg ttattctccg ggcccggaga
     3721 cgcggcggac gcggtccagc gcctcccgac ggaaggaatc cagcaggcga gccacataac
     3781 ggcccgcatc caggccggga tcgcggagca acggggtgag gtccatggcg agacacagct
     3841 cccgggcccg gtccggcgcg tacgggtcgg caaaggtcgc gttggcggcc cggcgcccgg
     3901 ccgccgcggc gtcgtaacgc ttcccgccct cgatctgcga gcggaacacg cggatcagtt
     3961 ccgcggcgag agttcctccg gagctgacat tcagttcgat gcggccctcc gcgggcagcc
     4021 agtcgaggct gccccagacc tccacgccgt acaggctttt cggccgccgg tcggggggca
     4081 ggcggcgcag cgcgcgcacg accgcaagcg acacggccac atgggtctcg tgccggtcgg
     4141 cgggatggtg ggtatagacg gtttccggcc gcgccgcccc gaacaatgcc tggatgtccg
     4201 cctccaacgc ggacacggcg gggcccttca aatcggcgct gcggtgatcc agcatcgcca
     4261 ggatgccgta gcggcccatc cgcgcggcct cctcctgctc ccggcggcgc acgtcccgga
     4321 cgcgttcgcc tccatgggcg cagatcacgg cgccaaatcc ccgggtctcc cggcacgcga
     4381 ggattccgtg cagggcgatg atctccgcgt cgtcgggatg cgccccgatc cccaggtgcg
     4441 agatcccggc caaggcttgg tccgcgggcc ggccgtcggg cacgaggagc gacgcctgtg
     4501 gttggctgaa cttcatgccg gcgatgatac tggatcggca aacagccgaa agaaaaaaca
     4561 aggcgcgcgg gcggccggct ctatgggacg gggatcgcct cgccgccggc ccgcaccttg
     4621 gtttcgagct ggtccaggta ctgcccggcc tgctcgccgg cctcgggcgg atcggaacgc
     4681 atgaacgcga ccatgagatc gcgggcctct tcgagccggt cctgctcctc gtacatcttg
     4741 gccaggcgca tgtgggcttc gggcgagaac cggtacaggt cgatggcctg ccggaacgcc
     4801 gcctcggcct cgtcgtactt cttgcgatac acgtacaccc ccgcgatggc ggagcgcagc
     4861 ttggagaagg ccttccgggc atcggggttg gacgcccagc ccggctggct ctccaggcgc
     4921 gccagggtgt ctttccagaa ctgccggtcg cgctcgacgg cgtccgccgt cagctcgggc
     4981 aggggctcgg ggttcaactt caggataagc ccatgcggct cgagataggg gtacatccag
     5041 tccacgacgt agctctcctc caggaagaac gggtgctcgt ccttgttgcg ctcgaagatc
     5101 atccggcaca ggatgccgtt gatcatcatc acgccggcca cgccctggac cgtgacgcgg
     5161 ccgtcctcga aactgacctc ggcccccgcg ggataccgcc cggcctgcac gtcctccacg
     5221 tattcctgga acgcctggtt ggagtcctcc tgctccggga tccagaggcg gtcgccgtac
     5281 acggcgcgca ggtgcgccat gtacgtgttg tcggccaggc cgttctgggt gaggacgaac
     5341 accggaggcg gttgctttaa agcgttggcg gtcgtgacga cgaaccggcc gcagtccgtc
     5401 ccgccgaagt agatgctgcc ctccggcatg gaggacagga tgggctcgta gaacatccgc
     5461 aacagcgcgg agtcctgctt ccacccgacc cacacctccc atacgcccag cgtctcgtgg
     5521 acggtcgccc acaattcatt gcgcatcgac ggaacggacc agccgctgtc ccccggcgtt
     5581 ttgagcttct ggaatcggtt cgatgtcgcc gcccagtcgc ctgcctcggc ggcctcgaag
     5641 aaggacacgg ccttcttcgg caggacgagg tcgagctgct cggccagttc gacggccagc
     5701 ttgtgccgcg ccgcgctgaa ctccttcacc atggcgccga agtcgtccgt cggcatggcc
     5761 tcttcgctca tgaccggggc cgaggagagc gccgtcaacc ccagcgccag gcatccgatg
     5821 atcgttgctt tcatgccttc catcctttcg atcgtcacca tttgacactc gatcgcttca
     5881 tttgtttcat cgccgacttg aaaaaacgcc cgaacgtctt gtccttcttc ctcttttcga
     5941 cataggagag gttgtggtat tcggcttcct tcttgagttt caggtagccc tggtatcgct
     6001 cttcgctcaa ctcgccgtcc tgcaaggcgg ccaggaccgc gcaaccgggc tcgctcgtgt
     6061 gcgtgcagtc cgggaagcgg caccgagttg aatagtcggc gatgtcgtgg aaggtcgcgt
     6121 cgatgccgtc ctcggcgccg aggaggccca gttcgcgcat gcccggcgtg tcgatcagca
     6181 tggcgccgtt gtcgaggacg agcagttgcc ggcgggacgt tgtgtgcact ccttcgccgg
     6241 tgccgctcac ggcccgggtc tcgaaagcct ccttgcccag cagccggttg atcagcgtgg
     6301 tcttcccgac gcccgatgag ccgagcaggc agtaggtttt gccgggagcc agggccgccc
     6361 ggaaagcctc catcccggcg cccgtggcgt tgctcaaccg ggcgatcggc gcggaaatcc
     6421 cggacctccg gatatcctcg atccgtcgct ccagttcttc cggcgagacc aggtccgtct
     6481 tgctcatgat gatgtgagcc cccatgcggc catcgcgggc catcaccagg taccgctcca
     6541 ggcgcggcac gttgaaatcg aaatcgcagc cctgcacgat gaaagccgtg tcgatgttgg
     6601 cggcgatcat ctggaagtcc acgactttcc cggggcgctt gcggcgaagg agtgtcttgc
     6661 gcggccacac ctcgtggatg atcgccgggc cgtccgactc aggccgttgc gcgcacaccc
     6721 agtcgcccac gcaggggagg tcgccggccg agcgcgccgt gaaccggaac ttgcccgaca
     6781 gctccgcgta ccgctcgccg tccgggtcgc gtatcaggaa ggcgtcccgg tccacggccg
     6841 tgacgcgggc caggcatcgc cccggcggca ggagaccggc gacccgctct tcgaaccagg
     6901 agtcgaatcc aagggaagtt aattccatgg gcgtcttatg atcgaggtcg acccgtcttg
     6961 ccatcagcgg ctaactgccg ctaacggggg aggttcccga tgccatggca cagactagtc
     7021 ccttcgcccc ggctcttcaa gggcagatgc ctccgcagga ttccctcctc gaaatggaac
     7081 aggcttttcc cggttggcct tcaccatcac gcagatcgga aaacataccc gcggcgggtt
     7141 cacggaacga ttcctccctc ggccggatcg gcggcctcgc gcgcggcggc attggccgcg
     7201 caggcgagaa ggcgtaacca ggccgcccgc ttcccgccag cggcaaactc gcgaaagccc
     7261 tcaatagctg accggggccg cgaatccgcg cccgctcgcg cttccgcggg aatcgcgatg
     7321 gagcgaggct ttcagggcaa tcgctccagc gcttcatcga tgaccaggtt ggcgggggcg
     7381 gcgctccaga gctggatggg cgtggagatg cccaggggct ccgcctgcca gattccctct
     7441 cccgcccggg cgggggcgta cttgaacacc tcggaggtgg cgagcgggcc cagtttcacc
     7501 agggcggctt tcgttttcaa caggattttc cggaacagtt cgacatcctc ccgcgtcagc
     7561 ccgtcccgga gggttccgtc cgcatcgatg ctccgctgaa cgagttgatc gatgccgatg
     7621 atcagggcaa acagggtcca gggccaggcg gcgcggccgt gatactcgtc cgggccgaac
     7681 ttgacccagg cgtttttccc gttgtcctgc agggccggga tgtcccgcgg ggcgtatacc
     7741 gtgttggtga tggccatgcc caggtcctcc tccatgaaac cgaggccgaa cggataggaa
     7801 agcacgagcg gccggatgat ttgccggact tgctccatgg agggctttcc gaacaagagg
     7861 gcaaacacgt cgtcgcagtg catgagcggc agcggattac actcggtatc cagcagcagc
     7921 gcggtgaatt cgatgccgtc cttgatggcg tcgggcacgc ggtcgccgcg gaggaaatca
     7981 gccacggtga cgccgtccac ccggccgcgc aaaatggccg cccgttcgcg gcagccccgc
     8041 tcgcggtcgt cccaggtcac ttcaccgcga gccaggccct ccaggtagcg ttggaggtcc
     8101 cgacggatat cgcacgccga acgccggacc aggaaatgcg cccgcaccag gtccgggtcc
     8161 cacgcctccg cggcggcggc aaaacggtcg ggcttctcga taaattcgcg gatgacgtcc
     8221 agggcgtttc ctttcgcctc gcccagcagg ccgtcgccga gcccgagggc ttgtatccgc
     8281 gcgatcatct cccggatggc gcctatgacc atgggcagga ggtgcacgtt gatgtcctcg
     8341 gagcggaatc gccatgggag attgtaggtg tcccgccagt tggcggcgcc agcgaccgag
     8401 gtaatcagcg acgagccggc cgccttgaac tcgtccggat acgcggcgat aagttgcgcc
     8461 ggggtgagat ccggatactt cgtacgcagg ctttgccaag ctgcaacgta gggggccgcg
     8521 gcatgaagca ggtagttcca gttccgcaaa agcgtgaccc tgttcgattc cctccggccc
     8581 agcaccagat gctcttcctg cagccactgc gacaggtcgc cgtcgccgat ttccccgaac
     8641 cagaaggccg ccgcggaggg gaacatgtag gtctggtcca gcacgtcgag gaagggatgc
     8701 tccggaacgg ttccctgcag gatcgtctgc atgagcggcc gggcccggtc caggtcgccg
     8761 cggccgtacg tgtggaaaaa gccttcgagg gcatccatga cggcgcggtc gtcggtccac
     8821 tcgtccgtca cgttgacgat gcccccggcc gaaatttcgt ccgcgacgct ttggatgccg
     8881 gcgcgccggg cctgcgcgga caggacgggc cacatgatgc gcagcgagat gagcgtgtcg
     8941 cggccgaagt aggacaggaa attccaggag ccggcgagga acttctcgcg gtaggaaagg
     9001 aattcgaaat tccggatgga ctcggcgaat agggggtctt tcgccgccag ggcccggccg
     9061 cgttcgttca ccagttccgc gaacggcatg ggcgtgagca gggcaaagtc ggtcgaggcc
     9121 cggaccagca ggcggagcgg ggcggtgccc ggcggatcct tccgcgtgat gcgcaggcag
     9181 tcgccgcgca cgcggacatc ggtggcgggg ccgccggcga actccagccg gtagcggtgt
     9241 cggccgtcca acgcccggcg cgtgaaggtg atcgtgtttc ggcccgcgtc gaaaacggga
     9301 ccgacggtct gtccgcggtc ggccaggtcc cgcagggaga aacccaggac ggattcgatt
     9361 tccccgatgg cgcccgaccg gacaaggtcc ttcaagcggt tctcatggcg gcgatcgggg
     9421 tcgtcgccga accgggcgac caatgtatgg ttgaggagga tcgacgccgc cgtgatcaaa
     9481 agctccggcg aggccgacgc gagggtgaat tgcacgccgg cctggccgtc cgcgtcggcg
     9541 aaggggaggg gggccccctc cagggtccag cccgcaggct gggtggtctc gaagcacaag
     9601 gcgcttttgc cggccggcat ggccaccacg acgcggaggc cgcttttgcc gacgggcagt
     9661 tgaaggtgcg cggcgacgcg ggtctgcgta tcccgtacca ggaagttatg gaaatcaccc
     9721 tccgtttttt tcagatgcag cgtgctcatg gcgactctct ttgaaggggg ccggtcccgg
     9781 cgatcaggtc accaacgtta tgcttgaggc tcgccgcccc ggagttcttc caactctcgg
     9841 caggctattt ggcgtggcct caaggcggtg gtccggcaat cagtcttcgc ccagtgcttc
     9901 aaggtcggcc aagtccttct tccgacctgt ggcgcgcttg ttctgcgcgt actgatctcg
     9961 tccgaggtaa aggacgtcca catcgccgta cttccccgag actttgcccg cgttggcttc
    10021 ttcccacgtg acgccggtga tggaagtgat gaggtcaatg cggacggggg ggactccaag
    10081 ttgaatgact tgctcgggct tctggaagtc ttcttccttc agcccgagcg aaccgaagcc
    10141 aaaggaagag agagcggcga tgatgcgtgc ggcattgttc ttggtggggc gcacgtagag
    10201 gtcgatgtcg cccgtgaatc gcggagctcc atgatacgct agcgcataag ctccgacaat
    10261 gatgtactca atcttttgct cgttgaacaa cgcgagcagt tctttgaagt cgggttgaac
    10321 ttccatgtgc ttgcctgcga agtgactcca cagcgcttac ccgttccgat gcgtcacggt
    10381 tgcgccagaa cgcaagatct tgttgcgcag acgattcgtg aagattcctc accgaaacgc
    10441 tcttctgaat cattaagtta ttatatcagg gttgtggtgt ctctgcacca tcttcttctt
    10501 gccgcacgct tggcgtgacc ctcggttcac ccgaagtccc cgcgccaaac gtcgtcgaga
    10561 cgccttaaga caaccgaagt ggcatcggat gagagcagtc gataccgttt gccactaccc
    10621 gaagtgaaga cgtcctcttc caacgaccat ctgccgctca ccgcatttac agtaccgctc
    10681 attgttccat ctgcgttgat ggtgaactgg gtgtctcgct gatctgcgac gcgccatctc
    10741 cccacgagca tgctacgcgt cttctcgacc tttgcgcgct ctgcttcgat ttcctccctg
    10801 ggcactctct tggcgtgcca ctcgcctatc acccatgagc atgaagggat cgaggctgtt
    10861 ggtgtggcgc cgccgatgca gatagttccc ggacaccgtc actctcacca gcccgttatg
    10921 gctcggcggc gcggttcatc agctgaccgc gctggggcga cacggaatgg gaactgctgg
    10981 tggcgacgga aacatcgtcc catgtgatgg gctcgaccat ggcatcggcg gcctgcaatt
    11041 gaccgagatg gtcgatcgcc atgccggagt cggcgctttc gatgtgcatg ggatgtccca
    11101 gcggctcacc gatgccggcg attttcgcct gcatggccgg agtcaattgc ttgaccagtt
    11161 ccacgttgta ggtgcgtgac gacgtgtcgt cgagggctat tcgtccaaat atccgctcgg
    11221 cggcaatgcc gtacgttcgc gagtcccacg cgggtgaatc ttcttcgagc acgggatcaa
    11281 cactggtcgt caagatggag atgctgttgt cgctgttgcg gagtatctcc caggcgcaca
    11341 attgctgggg gaagtcccgc aacgagacgg tttcgacctc ccagaagcct cgttccggtt
    11401 gggccgggtc cggcgaagga aacgccgtaa ccacgttctg gtggcgatgg cccgccatga
    11461 ccaggaggag attcgggtaa ttgtgaagcg tggcgaccag gttgctttcg gatgcaggat
    11521 cctggaatgt tgaagatcgg cttgtgttgg tagcgctcgt ttgcggcaga atgggaatgt
    11581 ggcaggcaag gatcatgagc tgatcctcat tttgccccat ctgcaattcg ttggtaagcc
    11641 agctaaaacg tgcggcatca acccagccgc caccataaaa aagcgcgctc tggcccggca
    11701 cgtccgcctt gcaagtattg tcgagcacga tcaccttgat cggcaggttc gtcgcgggct
    11761 cgaacgaata gcaggccgcc aggctgccgg tgacgccggg agtgaaacca tgcccctgcg
    11821 gaagcgaggt gctatcaaag aacgcattga caaagttcgt gggtgaattg agcggcgtcg
    11881 agagcatgcg gcgatccgga tccgcagcga ccgttggagg cgtttcgaaa aggttcgttg
    11941 ccccccattt gatcacggtg ccataggggg tggtgccatc cacaaccccc acatacatgc
    12001 cgattccctc ggcgcccggt ggaatcaggg ggccgttggg tgaaatattg agcacgttgg
    12061 agcccaccat ggcttgctgg attttttcgc ttggataacc gatgcccatc cacagctgat
    12121 cgtggttgcc gatgaccgcg taccatggga tggagcgatc gagcccggcg gcttgatagg
    12181 gcatctggta atctatggtg tcggctccgt catgggcgcc cgaactgggt gtaatctgct
    12241 gtccgtccat gacttcaaca aaccagcgca gttcgttgta ttgaccggag ttgcacatgt
    12301 ccccgaggac aatgccgaaa tcgaaagacg ccagccggtg cagcgcattg atggtcttga
    12361 cggcggcatc gagacggtgg gtcgtggcca aaatgaccgg cgtatacgca gatggattga
    12421 gttgggcaat gccagcgtcg ccaaaatcgg cgctccaacc gaggcaggga acctgagccg
    12481 gggattcctt gtcggtcata tgaacgtcgg ccagggaaaa gaaagataat agccgcgccg
    12541 catggttcgt gcccgtatag ccttccggca tcaatgtcag cagccggccc tcgttcgtcc
    12601 ccggccccca atcccactgg ccgtagggcg aggtcaggta ggcggccgcg ttggacgggg
    12661 cgaccggcgg cgtctccggc gcaacgacgc gcggcaggat cgtttgttca cgcgtcccca
    12721 gctcgactcc gcttccggaa accggaagca acaaaaccaa cacgctgatg aattggatga
    12781 cctgaaactt ttgatatcca caccaccggg aaggtttctt catgctgttt gtcctttctt
    12841 tctgctctga tctttgtgag cggcccccgc gaacaaggtt aggctgcaag ccattgttcg
    12901 ggcaaaattg ttcctaacga gtgtgcgctt cgacgtatga gcgcaatacg gcgttcatcc
    12961 ttgtctgata ctttcgtcct tgctgacgaa accagccaag aacatccggg tctatcctga
    13021 tggacacaag ctccttgggc ttgggcattc ggataaccat ttttttgaag atactcggat
    13081 caatcctcgg gacgccagag gcgtcgatgt cctcgtcctt catcgaattg agtcgtttcc
    13141 agtccgttct agatttcatc tctgtagatc tgttcttcat gttctgtcgc ctttctggcg
    13201 gagattatga ggatggtgtc ttcctgccat tcgtatttca cggtgattat gtatatacgg
    13261 acagtagtta cgtcaagagc ctgtttccat ctgcccgaac gttacgggtg acgcggcacc
    13321 gcgcacacct tccgccacag ttttgccagc cacctgctcc tgaataatta cgacagccgc
    13381 accatccggg agatgctcgg gcacagcgac gtgcgcacga cgatgatcta tacccacacg
    13441 gtccagagcg cacccggaag gagcgggaaa gccccccgcg acttcccccc agggccggtc
    13501 agcgctccac cgggatgacg atgccgcgga ggatgatctt ctggcagaac aggaagagca
    13561 gcagggtcgg cagggcggcg acgaggagcg aggcgtagat cacggcctgg ccgctgcgcg
    13621 cctgcaactg gtagagccat accatcagcg tccacatccg ctcgtcctgg cagatcagca
    13681 gggcgaacat gaagttggag taggcgacgg tgaaggcgtg cagcgcgatg accgcgagga
    13741 tcggc
//
LOCUS       DAOOTK010000088        13741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2985_length_13741_cov_4.082274, whole genome shotgun sequence.
ACCESSION   DAOOTK010000088 DAOOTK010000000
VERSION     DAOOTK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_13741_cov_4.082274"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PK634_09990"
     CDS             <1..493
                     /locus_tag="PK634_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOW98106.1"
                     /translation="AFVLFTSAQLMKKVAELSRDALESAGLTMLVQGAGMPRHVMLER
                     FRRGGGAVLFGLDSFWMGVDVRGEALSNVIITRLPFAVPDQPLIKARMDRIREKGGDP
                     FRDYSLPEAILKFRQGVGRLIRTQTDEGIIVILDSRILAKWYGKMFLSSIPECPVEIV
                     EGD"
     gene            complement(521..1717)
                     /locus_tag="PK634_09995"
     CDS             complement(521..1717)
                     /locus_tag="PK634_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOW98107.1"
                     /translation="MSRNAYREARHPGRATAMLAVAALLRTGAPALADTTHSLVVRGS
                     NRTFIVHVPPSYDGSRAFPVLLMFHGLDGTAAGAASSYYDWQATADTNRFIVAFPESL
                     SPAGKDIPGFEDYDGTGKRWDVAHVFSNRVDSQDVEFTGAILDWMETNYNVRGSHVFT
                     TGHSYGAFFSYYVSACLDGRVKAFAEHSGGLKSYTFILTFYWPLDVSSPPPSVPGFLL
                     HSPGDGTVAYSNSVLLQTEMTAHGQTNLLVTLAGGLGHGWDKTKNQAQWDFFMAQTPM
                     IDDDADGMPDTWEWQHELDLGKDDSGSDPDGDGASHLEEYRAGTDPKDSSSVFKAETL
                     ASAMPDSVIIRWASATGKTYTVSAATNLGGGFTALTNGVPATPPVNVYTGGSPESAVY
                     FRVAIP"
     gene            1844..2434
                     /locus_tag="PK634_10000"
     CDS             1844..2434
                     /locus_tag="PK634_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HOW98108.1"
                     /translation="MSREMHIQEKIEALRRFHRQEGRAPGYAEMLALFKYRSKNAVHG
                     LLKKLQEHGLVRKSASGKLALTSRLTGSVRLLGAVQAGFPSPAEEELADTLTLDEFLI
                     RRPEATYMLTVTGDSMTGAGILPGDIVLVEKGGTPKPNDVVVAQVDDEWTLKYFNRDR
                     AGVRLDPANPKYRFIRPRRSLSIGGIVRAVIRKYGN"
     gene            2471..2890
                     /locus_tag="PK634_10005"
     CDS             2471..2890
                     /locus_tag="PK634_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOW98109.1"
                     /translation="MKENELTREIIAAAMRVHSALGPGLLESAYEACLAHELSRHGLA
                     FERQKALPVMYDGIQIDCGYRIDLLVNDSVMVELKAVDVLLPIHHAQLLSYLRLSGKT
                     LGLLINFHEAHLRDGIVRKINHPRRTSEPSAVQHVSP"
     gene            2877..4193
                     /locus_tag="PK634_10010"
     CDS             2877..4193
                     /locus_tag="PK634_10010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOW98110.1"
                     /translation="MSPHENPLLTLHSYPRAIVHFDGDAFFASVEQAVHPELKGRPVV
                     TGRERGIIACASYEAKALGVARGITLAEARKKCPELVILPSDYETYSLYSKRMFDIMR
                     RYTPVVEEYSIDEGFVDLSGLRRVFHTSYEEIARRMQQDVRAELDITVSVGLSLSKGL
                     SKLASKFRKPAGFTAVAGNHLHLFLPRIALEKVWGFGPNTVHLLKKYGLQTAWDFVGR
                     PESWAEKMMGKIGREIWNELRGVAMHPVSTEEKSTYYTISKCKTFAEPTRDRDQVFAR
                     LTRNVESAFIKLRRHRLRAREIQVTLRFQDFTYEGIEARLQRPTAATQEVMPLVRDLF
                     DRVWRDGAEYRATIVVLGRLEPDRGEQFELFEDRVRIDKMSAVSGVIDAVNERYGKHT
                     LSLGASLFLGRKPTTGRGTLPWRKRELLGGETFRQRLKIPRLGVQV"
     gene            complement(4250..4897)
                     /locus_tag="PK634_10015"
     CDS             complement(4250..4897)
                     /locus_tag="PK634_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOW98111.1"
                     /translation="MPVVRQELAEYARKIAAAGLTAGAGGNISAREGRLVWVKPSGLA
                     MAELKGTDLCAVDIASGRQVEGRLRPTSELPMHLAVYRARPDVQAIFHTHSPFASGVI
                     SSTDDIRPMFAEVVNDLGGVALVPYIMTSTQALADAVAAAAKDSGTIFMANHGVVALG
                     RTMRQAFFRCEVAEDAAKALVAARLAGKPAFLTDAQVEALKKLEGGAHRKRMMER"
     gene            5041..5484
                     /locus_tag="PK634_10020"
     CDS             5041..5484
                     /locus_tag="PK634_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019506117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOW98112.1"
                     /translation="MNTILALVDFSDVTDAVVEQAASMAKGLEAEVVILHVAEPDPDF
                     VGYEAGPQTVRNTMAEHFRDEHRSLRHLEDKVKAQGVAVTGLLVQGPTVEKCLAEAAR
                     LQPLVMVIGSHGHGLLRDLLVGSVTEGVLRKAACPVLVVPVRAWE"
     gene            5619..5885
                     /gene="ffs"
                     /locus_tag="PK634_10025"
     ncRNA           5619..5885
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK634_10025"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            5911..7611
                     /gene="dnaX"
                     /locus_tag="PK634_10030"
     CDS             5911..7611
                     /gene="dnaX"
                     /locus_tag="PK634_10030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOW98113.1"
                     /translation="MAYEVLARKWRPQQFDDVIGQGHVTQTLKNAIASNRVAHAYLFV
                     GPRGVGKTSTARILAKALNCEKGPTPEPCGKCAACREIAAGNCLDVIEIDAASNTGVD
                     NVRDLRENARYAPARGPFKVYIIDEVHMLSVAAFNALLKTLEEPPAHVKFIFATTEPQ
                     KVPATILSRCQRFDLRRISARDIVGRLTEIAKAEKVSVDPDALLAIARGAEGGLRDAE
                     SALDQLIAFRGKAIKEDDVLAVFGLVARQTLDELMEAVLKGDVATAVRRVGDLDRSGK
                     DLQRLVFELLEQARNIVICQYTGADPAGLEVTEAQAESIRRQAAMCDAGRVLRVVDIL
                     TEVEGRIRYALSRRALLETALIRSGRAAVTVTLDELLAQVNQLKAGLGAGANDPSPAA
                     APARAEPAAARPPASPPKNPAKDARPASEEVAALAGGWLDIVERVSRLAPLAKSYLLD
                     GKPIHVQGARVIVGFDPEFASNKEKIEYPRNLKAVNKVLGEVLRREVTVEFRVLDAKA
                     TVPGDIKVREVAAPPGKTEPAAEEAQRPRGRSAQEWVKNPAVKKTLELFSGDIVDIRE
                     "
     gene            7637..7945
                     /locus_tag="PK634_10035"
     CDS             7637..7945
                     /locus_tag="PK634_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOW98114.1"
                     /translation="MVNILKMMKQAASMQKNMETLQADLAGRTVEFSSGGGMVTAVAR
                     GDGTVAEIRIDPKVVDPADVEMLQDLVLAAVDGAIKGARDMAAAEMAKLTSGLGLPSM
                     "
     gene            7952..8563
                     /gene="recR"
                     /locus_tag="PK634_10040"
     CDS             7952..8563
                     /gene="recR"
                     /locus_tag="PK634_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOW98115.1"
                     /translation="MPASDPMGRLQALLGKLPGVGRRSAERMAQALVRDGENLLPNLV
                     AALREVEETVRLCSLCGGLTLKTQDPCALCRDPRREASQLCVVEEPSDIPLIERAGAF
                     RGRYHALLGKISPMQGQGVPDRRVEALLKRIDSEGVREVILALNSDVESDATASYLAD
                     VLAKRPVKVTRLAFGIPAGSAISFSDPVTLARALQGRSPVGPS"
     gene            complement(8558..9052)
                     /locus_tag="PK634_10045"
     CDS             complement(8558..9052)
                     /locus_tag="PK634_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOW98116.1"
                     /translation="MTAQQIIEKLGLVPLPGEGGYYRETHRSKTLLPRAALPAGYPAG
                     RAASTAIYYLLTPETSSALHRLRGDEVWHFYLGDPVEQLQLLPDGRSRIVVLGSDLGR
                     GQQPQALVPGGTWQSARLLPDGRFALLGTTMAPGFEFEEYEPGNPAELAKAHPLLDRW
                     LKKL"
     gene            complement(9049..9777)
                     /locus_tag="PK634_10050"
     CDS             complement(9049..9777)
                     /locus_tag="PK634_10050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW98117.1"
                     /translation="MTDSGHLGKLAVVTGATKGIGRAIAEKFAAGGFDLAFCARTAAD
                     VQAMEADLCRRFPGQRFLGRAADLSSCADVDGFARAVVEQGRAADVLVNNAGVFIPCP
                     VADPAGRESFDLMMRTNVSSAYWLTQGLLPPMRAARRGHIFNLCSIASVTSYGGGYGV
                     SKFALLGYSKNLREELKKDGIRVTAVLPGATLTPSWNGVNLPADRFVRASDVADAVWA
                     CYALSPQAVVEELLVRPQLGDIEI"
     gene            9924..12545
                     /gene="alaS"
                     /locus_tag="PK634_10055"
     CDS             9924..12545
                     /gene="alaS"
                     /locus_tag="PK634_10055"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOW98118.1"
                     /translation="MTASEIRKSFLDFFRGKQHAIVPSSPVVLPSDPTLLFANAGMNQ
                     FKEIFLGARQSESPRVADTQKCIRVSGKHNDLEEVGLDTYHHTFFEMLGNWSFGDYYK
                     REAIAWAWELLTEVWKLPRERLWITIYRDDDEAERIWKATPGVLPSHVLRFGEKDNFW
                     EMGETGPCGPCSEIHIDRTANGCTPDMVNAGSPEVMELWNLVFIQYNRRADGTLEELP
                     AKHVDTGMGFERLVSVLQGKPSNYDTDLFMPMLDRLAQMTGEKYEGKSAVAMRVIADH
                     LRALSFAIADGVLPSNEGRGYVLRRLLRRAVRYARKIGLRDPFMGELFPVLEGIMGDV
                     FPELRRRREDILRALRAEEESFAAALDRGTGLFDDVVKGLKARKEDTFPGDEAFKLYD
                     TYGFPLDLTILMAGENGLKVDQEKFTELMEQQRERARSARTEKMRKADADVVADLVSR
                     GLKSVFTGYETLAGESEVLAVLSDGKDRGELAEGAEGELLLARTPFYAESGGQIGDKG
                     VLRGPAGEFEVWDTQRPAEGLILHIGRVTQGCLKRGEKATALVDADRRNRTARHHSGT
                     HLLQHALRELVSPSIKQAGSYVGPDRMRFDISYFEAVPEDKLDAVERRVNELILENVA
                     LKIYQMPFKDVAGSGIIALFDEKYGNAVRVVEVPGYSRELCGGTHVRATGDLGLFRIV
                     SESSVAAGVRRIEAVCGRPAYEWTHREHRLVGTLSRRLSASPDEVFARVEALMEDNRR
                     LEKELKQQATAGALNRVDEWLGRARKVGEIPLIAEDVGEQSMDVLRGVMDGLRARFAS
                     GVIVLGSRFEGKACFLASVSEDLQARGVHAGKLIGAVAKVAGGGGGGQPGKAQAGGKD
                     ASKVAEALGKVPELLAR"
     gene            complement(12531..>13741)
                     /locus_tag="PK634_10060"
     CDS             complement(12531..>13741)
                     /locus_tag="PK634_10060"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOW98119.1"
                     /translation="ETADTLERLARLKKRGRPRKIIALGCLVERAGQEPTLAGFLKQA
                     DARVSFSGYARLPQTCRDLMQSGVVPGSPSRGCDFTAFLKTPRARIGGVHSAYLKISE
                     GCSNRCRYCSIPLIRGAQVSRPMEDILAEAGQLIRHGAREVNLVAQDTTAYGADLYGQ
                     ARLPELLRRLGELDGAAWFRLLYAHPARLSDEVIDLVAGAPHLCPYLDVPLQHISDPI
                     LERMGRRIGQAQTLKLLERIRSRMLHGALRTTFIVGFPGETKADFERLLHLVRDGWFT
                     HAGVFTYSSEPGTPAADWPDDVAPGEKERRRDALMQAQREISKAKGLAQVGQTVEVML
                     DGVAERKGRLPHGAMAVGRTRLQAPEVDGTVLVRPPLPKPVPGPGDRVQAEIVEALDY
                     DLVGRFLPGQ"
BASE COUNT         2298 a         4525 c         4795 g         2123 t
ORIGIN      
        1 cgccttcgtc ctgttcacca gcgcccagct catgaagaag gtcgccgagc tgtcgcgcga
       61 cgccctggag tccgcggggc tgacgatgct ggtgcagggg gccggcatgc cgcggcacgt
      121 gatgctggag cggttccgcc ggggcggggg cgcggtgctg ttcgggctgg acagtttctg
      181 gatgggcgtc gacgtccgcg gcgaggcctt gagcaacgtc atcatcaccc gcctgccgtt
      241 cgccgtgccc gaccagccgc tgatcaaggc ccggatggat cgcatccgcg aaaagggcgg
      301 cgacccgttc cgcgactatt ccctgcccga ggccattctg aagttccggc agggggtcgg
      361 ccgcctgatc cggacgcaga cggacgaggg catcatcgtg atcctcgaca gccgcatcct
      421 ggccaagtgg tacggcaaga tgttcctctc ctcgatcccc gagtgcccgg tggagatcgt
      481 ggaaggggat taggggggag ggggtcaggc cgcaggggcg ctacgggatg gccacgcgga
      541 aatacaccgc tgactccggc gagccccctg tgtagacatt cacgggcggc gtggccggca
      601 cgccgttggt gagagcggtg aacccgccgc ccaggttagt cgcggcggac accgtgtacg
      661 tcttgcccgt tgcgctggcc cagcggatga tcacggagtc cggcatggcg gaggccaggg
      721 tttccgcctt gaaaaccgag gagctatcct tcgggtccgt gccggcgcga tactcctcca
      781 ggtggctggc cccgtcgccg tcggggtcgg atcctgaatc gtcctttccc aggtccagtt
      841 cgtgctgcca ctcccaggtg tccggcatgc cgtccgcgtc gtcgtcgatc atcggggtct
      901 gcgccatgaa gaagtcccac tgggcctggt ttttggtctt gtcccacccg tggcccaatc
      961 cgccggccag cgtcaccagc aggttggtct gcccgtgggc agtcatctcg gtctgcagca
     1021 gcacgctgtt cgagtaggcc acggtgccgt cgccgggaga atgaaggaga aagcccggca
     1081 cggacggggg cggggaggag acgtccagcg gccagtagaa ggtcaggata aaggtgtagc
     1141 tcttcagccc gccggaatgc tccgcgaagg ccttgacccg cccgtccagg caggcggaga
     1201 cgtagtagct gaagaacgcg ccgtacgaat ggcccgtcgt gaagacgtgg gagccgcgga
     1261 cgttgtagtt cgtctccatc cagtccagga tcgcgcccgt gaattccacg tcctgcgaat
     1321 ccacgcggtt gctgaacacg tgggccacgt cccatcgctt gcccgtcccg tcgtagtctt
     1381 cgaaaccggg gatgtccttg cccgccggcg acagggattc cgggaacgcg acgatgaacc
     1441 ggttcgtgtc cgccgtcgcc tgccagtcgt agtaggacga ggccgcgccg gcggccgtcc
     1501 cgtccaggcc gtggaacatc agcagcacgg gaaaggcccg cgagccgtcg taggagggcg
     1561 gaacatggac gatgaacgtg cggttcgagc cgcgcacgac gaggctgtgc gtcgtgtccg
     1621 cgagcgctgg cgccccggtg cgcaaaaggg ccgccacggc cagcatcgcg gtcgcccggc
     1681 cgggatgccg cgcctccctg tacgcgttcc ggctcatctc cgggggacaa catacccgga
     1741 ttccggcgga aaatacaccc cttcgcgttc tcccggaggt tttgcttgaa actgaacttt
     1801 cgttcagtta gggtgtacta tggagatggg aaaggagaag gccatgtcgc gggaaatgca
     1861 tatccaggag aaaatcgagg ccctgcgccg gttccaccgg caggaggggc gcgcgcccgg
     1921 ctacgcggag atgctggcgc tgttcaagta ccggtccaag aacgcggtcc acggcctgct
     1981 gaagaagctc caggagcacg ggctcgtccg caagtccgcc tccggcaagc tcgcgctcac
     2041 gtcgcgcttg accggctcgg tgcggctgct gggcgccgtc caggccggct tcccgtcgcc
     2101 cgccgaggag gaactggccg acaccctgac gctggacgag ttcctcatcc ggcgcccgga
     2161 ggcgacgtac atgctgaccg tcacgggcga ctccatgacg ggcgcgggga tcctgcccgg
     2221 cgacatcgtc ctcgtcgaga agggcggcac gccgaagccc aacgacgtgg tcgtcgccca
     2281 ggtggacgac gagtggaccc tgaagtactt caaccgcgac cgggcgggcg tccggctcga
     2341 ccccgccaac cccaagtacc ggttcatacg gccccgccgg tccctctcca tcggcggcat
     2401 cgtccgcgcc gtgatccgga agtacgggaa ttgaaccgca gaggacgcgg aggatcgcag
     2461 aggggatgaa atgaaagaaa acgagttgac gcgggaaatc attgcggcgg caatgagggt
     2521 gcattcggcg ttgggtcccg ggctattgga gtcggcctat gaggcctgct tggcgcacga
     2581 attgagccgg catgggctgg ccttcgagcg acagaaagcc ctgccggtga tgtatgatgg
     2641 gattcaaatc gactgcggat atcgcatcga tctgctggtg aacgattccg tgatggtgga
     2701 gctcaaggcg gtagacgtcc tgttgcccat tcatcatgct caacttttaa gttatctccg
     2761 gctttccggg aaaacattgg gccttctgat caattttcac gaagctcatc ttcgcgatgg
     2821 aatcgtaagg aaaataaatc atcctcggcg aacctctgag ccctctgcgg ttcaacatgt
     2881 ctccccatga aaatccgctg ctgaccctcc attcctaccc ccgcgcgatc gtgcatttcg
     2941 acggggacgc gttcttcgcg tccgtcgagc aggcggtcca cccggagctg aagggccggc
     3001 cggtcgtgac ggggcgggag cgcgggatca tcgcctgcgc cagctacgag gccaaggccc
     3061 tcggcgtcgc gcgcggcatc acgctggcgg aggcccggaa gaagtgcccc gagctcgtca
     3121 tcctgcccag cgactacgag acctacagcc tctactccaa gcgcatgttc gacatcatgc
     3181 ggcgctacac gccggtcgtg gaggagtact ccatcgacga gggcttcgtg gacctctccg
     3241 ggctgcgccg ggtgttccac acgtcctacg aggagatcgc ccgccggatg cagcaggacg
     3301 tccgggccga actggacatc accgtgtccg tcggcttgag cctctccaag ggcctgtcca
     3361 agctggcctc gaagttccgc aagcccgccg ggttcaccgc cgtcgcgggc aaccacctgc
     3421 acctgttcct gccgcgcatc gccctcgaga aggtctgggg cttcgggccc aacaccgtcc
     3481 acctgctgaa gaagtacggc ctgcagaccg cctgggactt cgtgggccgg cccgagtcct
     3541 gggcggaaaa aatgatgggc aagatcgggc gcgagatctg gaacgagctg cgcggcgtcg
     3601 cgatgcaccc ggtgagcacg gaggagaaat ccacctacta caccatcagc aagtgcaaga
     3661 cctttgccga gccgacgcgc gaccgggacc aggtcttcgc gcggctgacc cgcaacgtcg
     3721 agtccgcctt catcaagctg cgccgccaca ggctgcgcgc gcgggagatc caggtcaccc
     3781 tgcgcttcca ggacttcacg tacgagggga tcgaggcccg gctccagcgg ccgaccgctg
     3841 cgacgcagga ggtcatgccg ctcgtccgcg acctgttcga ccgggtctgg cgggacgggg
     3901 ccgagtaccg ggcgaccatc gtcgtgctcg gccggctcga gccggaccgc ggcgagcagt
     3961 tcgagctgtt cgaggaccgc gtgcggatcg acaagatgag cgcggtctcc ggcgtgatcg
     4021 acgccgtcaa cgagcgctac gggaagcata ccttgagcct gggggcgtcc ttgttcctcg
     4081 gccgcaagcc gaccaccggg cgcgggacgc tgccgtggcg caagcgcgag ctgctgggcg
     4141 gggagacgtt ccggcagcgg ctgaaaattc cgaggctggg ggtgcaggtg tgatgccaag
     4201 gtcggccgcg acggcgcacg gccctcctgc atagccggag ggcgccgaat tatcgctcca
     4261 tcatccgctt ccggtgcgcg ccgccctcga gtttcttgag cgcctcgacc tgggcgtcgg
     4321 tcaggaaggc gggcttgccg gccagccggg cggcgacgag cgccttggcg gcgtcttcgg
     4381 cgacctcgca ccggaagaac gcctggcgca tggtcctgcc gagggcgacg acgccgtggt
     4441 tggccatgaa gatcgtgccg ctgtccttcg ccgcggcggc gacggcgtcc gccagggcct
     4501 gcgtcgaggt catgatgtac ggcacgaggg ccacgccgcc gaggtcgttg acgacctccg
     4561 cgaacatcgg gcggatgtcg tcggtcgagg agatgactcc ggaggcgaag ggggagtggg
     4621 tgtggaagat cgcctgcacg tcgggccgcg cgcggtagac ggcgaggtgc atcggcagct
     4681 cggacgtcgg gcggagcctg ccctccacct ggcggccgga ggcgatatcc acggcgcaca
     4741 aatccgtgcc cttcaactcg gccatggcca agccgctggg cttgacccag accaaccgtc
     4801 cctcgcgggc gctgatgttg ccgccggcgc cggccgtcag tccggcggcc gcgatcttcc
     4861 gggcatattc agccagttcc tggcgcacga cgggcatgcg ggctcctcga ttccactgaa
     4921 ccggatcgta tatcacaacg cttgcccggt aggaagtccc gtccgcgctc gtgaaagggc
     4981 tttccgggcg ggcatctttg tgctaaaaag aacatggcgg gaagccggct ggaggcaaac
     5041 atgaacacga tcctggccct ggtggatttt tccgacgtga ccgacgcggt cgtggagcag
     5101 gcggcctcga tggccaaggg gctggaggcc gaagtggtga tcctgcacgt cgcggagccg
     5161 gatcctgatt tcgtcgggta cgaggccggg ccccagaccg tccggaatac catggcggag
     5221 cacttccgcg atgagcatcg ttcgctccgc catctggagg acaaggtcaa ggcgcagggc
     5281 gtggcggtga ccggcctgct ggtgcagggg ccgaccgtcg agaagtgcct cgccgaggcg
     5341 gcgcgattgc agccgctggt gatggtcatc ggctcgcatg gccacggcct gcttcgcgac
     5401 ctcctggtgg gaagcgtcac ggaaggcgtc ctgcgcaagg cggcctgccc ggtgctggtc
     5461 gtgcccgtcc gcgcctggga atgacgccgt cccccgcccg gccttcagga ggataacaaa
     5521 acgcttcacg ggtgtgaaga cttttgttat cgcccttccg ggccgtcggg gcgccgcgac
     5581 ttgccaagga gcaggccacc cggtatgttt tcccacggcc gtgctagacg gggaggtagc
     5641 ggtgccctga acctgcaacc cgctatagca gggtggaatt cccgagccag gcggacgcgg
     5701 gagagttgaa cgtcccggca acggacgatg acggtcggcc cctatgcgac ggaaagcggc
     5761 aaaccccgtc aggaccggaa ggtagcagcg gtacgccgtg acattccggg cgccgtagga
     5821 acgctggctc gagcccggac ccggtgcacg cggccctccg gtttccgtcg aacgagggtg
     5881 cacggccctg tataacaacg gtgggtggct atggcatacg aagtactcgc tcgcaagtgg
     5941 cggccccagc agttcgatga cgtcatcggg cagggccacg tcacccagac gctgaaaaac
     6001 gcgatcgcct ccaaccgcgt cgcccacgcc tacctgttcg tgggcccgcg cggcgtgggc
     6061 aagacctcca cggcccgcat cctggccaag gcgctcaact gcgagaaagg cccgaccccg
     6121 gagccgtgcg ggaaatgcgc ggcgtgccgg gagatcgcgg ccgggaactg cctcgacgtg
     6181 atcgagatcg acgcggcctc caacaccggc gtggacaatg tccgcgacct gcgcgagaac
     6241 gcgcgctacg cccccgcccg cggccccttc aaggtctaca tcatcgacga agtgcatatg
     6301 ctgtccgtgg cggccttcaa cgccctcctg aagacgctcg aggagccgcc cgcgcacgtg
     6361 aagttcatct tcgccaccac cgagccgcag aaggtgcccg cgaccatcct gtcccgctgc
     6421 cagcggttcg atctgcgccg catctcggcg cgcgacatcg tcggccggct cacggaaatc
     6481 gcgaaggcgg aaaaggtcag cgtcgatccc gacgcgctgc tggccatcgc gcgcggcgcc
     6541 gagggcgggc tgcgcgacgc cgagtcggcc ctggaccagt tgatcgcgtt ccgcgggaag
     6601 gccatcaagg aggacgacgt cctggcggtc ttcggcctgg tcgcgcggca gacgctggac
     6661 gaattgatgg aggcggtgct caagggcgac gtggccacgg cggtccgccg ggtcggcgac
     6721 ctggaccgca gcggcaagga cctgcagcgc ctcgtgttcg aactgctgga acaggcgcgc
     6781 aacatcgtga tctgccagta caccggcgcg gacccggccg gcctcgaggt gaccgaggcc
     6841 caggccgaga gcatccgccg gcaggcggcg atgtgcgacg cgggccgcgt cctgcgggtg
     6901 gtcgacatcc tgacggaggt cgaaggccgc atccgctacg ccttgtcccg ccgcgccctg
     6961 ctggaaaccg ccctgatccg gagcgggcgc gcggccgtca cggtcacgct ggacgaactg
     7021 ctggcccagg tcaaccaatt gaaggccggc ctgggcgcgg gagcgaacga cccttcgccc
     7081 gccgccgctc ctgcgcgcgc ggaacccgcg gcggccaggc cgccggcgtc ccccccgaag
     7141 aacccggcaa aggatgcccg gcccgcatcg gaggaagtag ccgccctcgc cggcggctgg
     7201 ctggatatcg tggagcgcgt cagccgcctc gcgccgctgg cgaaatccta cctgctcgac
     7261 gggaaaccaa tccacgtgca gggcgcccgc gtgatcgtcg gattcgatcc cgagttcgcc
     7321 agcaacaagg agaagatcga atatccccgg aacctcaagg ccgtgaacaa ggtgctgggc
     7381 gaagtgctcc ggcgcgaggt gacggtcgag ttccgcgtcc tggatgccaa ggcgaccgtg
     7441 cccggcgata tcaaggtgcg ggaggtcgcc gccccgccgg ggaagacgga gccggcggcg
     7501 gaggaggcac aaaggccgcg gggccgctcg gcgcaggaat gggtcaagaa tccggccgtg
     7561 aaaaaaaccc tggaattgtt cagcggcgat atcgtggata tccgggagta ggcgcggcgg
     7621 tttttcagga gggatcatgg tcaatatcct caagatgatg aagcaggcgg cgtccatgca
     7681 gaagaacatg gagacgctcc aggccgatct ggcggggcgc accgtggaat tcagcagcgg
     7741 cggcggcatg gtcaccgccg tcgcgcgggg cgacgggacc gttgcggaga tccggatcga
     7801 tccgaaagtg gtcgatccgg ccgacgtgga gatgctccag gacctcgtcc tggccgccgt
     7861 ggacggcgcg atcaagggcg cccgcgacat ggcggcggcc gagatggcca agctgacctc
     7921 cgggctcggt ctgccgtcca tgtaggcgcg catgcccgcg agcgatccca tgggccggct
     7981 gcaggcgctg ctgggcaagc tgccgggcgt cgggcggcgc tccgccgagc ggatggcgca
     8041 ggcgctggtc cgggacggcg agaacctgct ccccaacctg gtcgccgcgc tgcgggaagt
     8101 cgaggagacg gtgcggctct gcagcctgtg cggcgggctg accctcaaaa cgcaggatcc
     8161 ctgcgccctc tgccgggatc cgcgccggga ggcaagccag ttgtgcgtgg tggaggagcc
     8221 ctccgatatc ccgctgatcg agcgcgccgg cgccttccgc ggccgttacc acgcgttgct
     8281 cggcaagata tcgcccatgc aggggcaggg ggttcccgac cgccgcgtcg aggcgctgct
     8341 gaagcggatc gattccgaag gcgtgcgcga ggtcatcctg gcgctgaaca gcgacgtgga
     8401 gagcgacgcc acggcctcct acctggcgga tgtgctggcg aagcgcccgg tcaaggtcac
     8461 gcggctcgcg ttcggcatcc cggccggcag cgcgatttcc ttctccgatc ccgtcacgct
     8521 cgcccgcgcc ctgcagggcc gctcacccgt cgggccgtca tagcttcttc agccagcggt
     8581 cgagcagggg atgggctttc gccagttcgg cgggattccc cggctcgtat tcctcgaact
     8641 cgaatcccgg agccatggtc gtgcccagca acgcgaaccg gccgtccggc agcagccgcg
     8701 cgctttgcca ggttccgccc ggcacgagcg cctgcggctg ctggccgcgc cccaggtcgg
     8761 acccgagaac gacgatccgg cttcgcccat ccggcaggag ttgaagttgc tcgaccgggt
     8821 cgccgagata gaagtgccac acctcgtcgc cgcgcagacg atggagggcc gacgacgtct
     8881 cgggcgtgag caggtagtag atggccgtgg aggcggcgcg gcccgcggga tagccggccg
     8941 ggagcgcggc ccgcggcaga agcgttttcg agcggtgcgt ctcccggtag tacccgccct
     9001 ccccgggcag cgggaccagc cccagctttt cgatgatctg ttgcgcggtc atatctcgat
     9061 gtccccaagc tgcgggcgga ccagcagttc ttccaccacg gcctgcgggc tcaacgcgta
     9121 acaggcccag accgcgtcgg ccacgtcgga ggcccggacg aaacggtcgg ccggcaggtt
     9181 cacgccgttc cacgacggcg tcagcgtggc gcccggcagg acggccgtga cgcggatccc
     9241 gtccttcttg agctcctcgc gcaggttctt cgagtagccg agcagggcga acttggacac
     9301 gccgtagccg ccgccgtacg aagtcacgct ggcgatggag cacaggttga aaatgtggcc
     9361 ccgccgcgcg gcgcgcatcg ggggcagcag gccctgggtc agccagtagg cgctcgagac
     9421 gttggtccgc atcatgagat cgaacgattc gcggcccgcg ggatcggcga cggggcaggg
     9481 gatgaaaacc ccggcgttgt tcaccagcac gtcggccgcg cggccctgtt cgacgacggc
     9541 gcgcgcgaag ccgtcgacgt cggcgcacga cgacaagtcc gccgcccggc ccaggaatcg
     9601 ctgcccggga aagcgtcggc agagatcggc ttccatcgcc tgcacgtcgg cggcggtgcg
     9661 ggcgcagaag gccaggtcga agcctccggc cgcgaacttc tccgcgatgg cgcgcccgat
     9721 gcccttggtc gcacccgtga cgacggctag tttcccgaga tgcccggaat cggtcatgcg
     9781 ataatcctcc ggaagcgttt gaacggatga aagcacaggg ggcaccacgg catccagtag
     9841 aatcgtcccg gcggttcgtg attgctttac cgcacgaaat ccgacatttt tacccggttt
     9901 ttcagaaaaa ggtgccgacg atgatgaccg catccgaaat cagaaagtcg ttcctggatt
     9961 tcttccgggg caagcagcac gccatcgtgc ccagctcgcc ggtcgtgctg ccctccgatc
    10021 ccacgctgct gttcgccaac gccggcatga atcagttcaa ggagatattc ctcggcgccc
    10081 gccagagcga gtcgccgcgc gtcgcggata cgcagaagtg catccgggtc agcggcaagc
    10141 acaacgacct cgaggaggtc ggcctcgata cctaccacca cacgttcttc gagatgctgg
    10201 gcaactggtc gttcggcgac tactacaagc gcgaggcgat cgcctgggcg tgggagctgc
    10261 tcacggaggt ctggaagctg ccccgcgagc ggctctggat cacgatctac cgcgacgacg
    10321 acgaggcgga gcgcatctgg aaggccaccc ccggcgtcct gccttcgcac gtcctgcgct
    10381 tcggcgagaa ggacaatttc tgggagatgg gcgagaccgg gccgtgcggc ccgtgctcgg
    10441 agatccacat cgaccgcacc gcgaacggct gtacgccgga catggtcaac gccggctcgc
    10501 cggaggtcat ggagctgtgg aacctggtct tcatccagta caaccgccgc gccgacggca
    10561 cgctggagga actgccggcc aagcacgtcg acacgggcat gggcttcgag cggctggtct
    10621 cggtgctgca gggcaagccc tccaattacg acaccgattt gttcatgccc atgctcgacc
    10681 gcctggcgca gatgacgggc gagaaatacg aggggaagag cgccgttgcc atgcgcgtga
    10741 tcgcggacca cctgcgcgcg ctctccttcg ccatcgccga cggcgtgctg ccgtcgaacg
    10801 aggggcgcgg ctacgtcctg cgccggctcc tgcgccgcgc cgtccggtac gcccgcaaga
    10861 tcgggctgcg cgacccgttc atgggcgagc tgttccccgt cctcgagggc atcatgggcg
    10921 acgtgttccc cgagctgcgt cgccggcgcg aggatatcct gcgggccctg cgcgcggagg
    10981 aggaaagctt tgccgccgcc ctcgaccgcg ggaccggcct gttcgacgac gtggtgaagg
    11041 ggctcaaggc gcgcaaggag gacaccttcc ccggcgacga ggcgttcaag ctctacgaca
    11101 cgtacggctt cccgctggac ctgaccatcc tgatggccgg cgaaaacggg ctgaaggtgg
    11161 accaggagaa gtttaccgag ctcatggagc agcagcgcga gcgcgcccgc tccgcgcgga
    11221 cagagaaaat gcgcaaggcc gacgccgacg tcgtcgctga cctggtcagc cgagggctca
    11281 agtcggtctt cacgggatac gagacgctcg ccggtgaatc ggaggttctg gccgtgctgt
    11341 cggacggcaa ggaccgcggc gaactggccg agggcgcgga gggcgaactc cttctggccc
    11401 gcacgccttt ctacgcggag tccggcggcc agatcggcga caagggcgtg ctccgcggcc
    11461 cggccggcga gttcgaggtc tgggacaccc agcggccagc ggagggcctg atcctgcaca
    11521 tcggccgggt cacgcagggt tgcttgaagc ggggcgagaa ggcgaccgcg ctcgtggacg
    11581 ccgaccggcg aaaccgcacg gcgcggcacc actcgggcac gcacctcctg cagcacgcgc
    11641 tgcgggagct ggtgagcccg tccatcaagc aggcgggctc ctacgtcggg cccgaccgga
    11701 tgcggttcga catcagctac ttcgaggccg tgcccgagga caaactggac gccgtcgagc
    11761 ggcgcgtgaa cgagctgatc ctcgaaaacg tcgcgctcaa gatctaccag atgccgttca
    11821 aggacgttgc ggggtcgggc atcatcgcgc tcttcgatga gaagtacggc aacgcggtgc
    11881 gcgtggtgga agtgcccggc tacagccgcg agctgtgcgg cggcacgcac gtgcgggcga
    11941 cgggcgacct gggcctgttc cgcatcgtct ccgaaagctc cgtggccgcc ggcgtgcggc
    12001 gcatcgaggc cgtctgcggg cggcccgcct acgagtggac ccaccgcgag catcggctgg
    12061 tcggcacgct gtcccggcgt ttgagcgcgt cgcccgacga agtgttcgcc cgggtcgagg
    12121 ccctgatgga agacaaccgt cgcctggaga aggaactcaa gcagcaggcg accgccggcg
    12181 ccctgaaccg ggtggacgaa tggctcgggc gcgcccggaa ggtcggcgag atcccgctga
    12241 tcgcggagga cgtcggcgaa caatcgatgg acgtgctgcg cggggtgatg gacggcttgc
    12301 gcgcgcgctt cgcctcgggc gtcatcgtcc tgggcagccg gttcgaaggc aaggcgtgct
    12361 tcctggcctc ggtcagcgag gacctccagg cccggggcgt gcatgccggc aagctgatcg
    12421 gcgccgtggc gaaggtcgcg ggcgggggcg ggggcggaca gccgggcaag gcgcaggccg
    12481 gcggcaagga cgcctcgaaa gtggccgagg ctctcggcaa ggtcccggaa ctactggcca
    12541 ggtagaaatc gcccgacgag atcgtaatcc agggcttcga cgatttccgc ctgcacccgg
    12601 tcgccgggcc cggggaccgg tttcggcagg ggcgggcgaa ccagcaccgt cccgtccacc
    12661 tccggcgcct gcagccgggt ccggcccacc gccatggccc cgtgcggcag gcggcctttc
    12721 cgttcggcca cgccgtcgag catgacctcc acggtctggc caacctgcgc gaggcctttc
    12781 gcctttgata tctcgcgctg ggcctgcatc agggcatcgc gccgccgctc cttctcgccg
    12841 ggcgccacgt cgtccggcca gtccgccgcc ggggtcccgg gctcgcttga gtaggtaaac
    12901 acgccggcgt gcgtgaacca gccgtcgcgg accagatgca gcagtcgttc gaagtcagcc
    12961 ttcgtttcgc cggggaaccc gacgatgaat gtcgtgcgca gcgcgccgtg cagcatgcgc
    13021 gaccggatgc gctccaggag tttcagggtc tgcgcctgcc cgatgcggcg tcccatgcgt
    13081 tcgaggatgg ggtcggaaat gtgctggagc ggaacatcca gatacgggca aaggtgcggc
    13141 gcgcccgcca cgaggtcgat cacctcgtcc gacaggcgcg cggggtgcgc gtacagcagc
    13201 cggaaccacg ccgcgccatc cagttcgccc agccgccgga gcagttccgg caggcgcgcc
    13261 tgcccgtaca ggtccgcgcc gtaggcggtc gtgtcctgcg cgacgagatt gacctcgcgc
    13321 gcgccgtgcc gaatcaactg cccggcctcc gcgaggatgt cttccatggg ccggctgacc
    13381 tgcgcgccgc ggatgagggg aatggagcag tagcggcacc ggttcgagca accctcggag
    13441 atcttcaggt acgcgctgtg cacgccgccg atccgggcgc ggggagtctt gaggaaggcc
    13501 gtaaaatcac agccgcgcga cgggcttccc ggtacaactc cggattgcat caggtcacgg
    13561 caggtttgag gtaaacgcgc gtagccgctg aaagacacgc gggcatccgc ctgtttcagg
    13621 aatccggcga gggtcggctc ctggcccgcg cgctcgacga ggcatcccag ggctattatt
    13681 ttgcgtggac ggcctcgctt cttgaggcgc gccagccgct ccagcgtatc cgcggtttcc
    13741 c
//
LOCUS       DAOOTK010000089        13695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_2996_length_13695_cov_5.144428, whole genome shotgun sequence.
ACCESSION   DAOOTK010000089 DAOOTK010000000
VERSION     DAOOTK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_13695_cov_5.144428"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PK634_10065"
     CDS             <1..995
                     /locus_tag="PK634_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98120.1"
                     /translation="RMLASLALNETTEENVRKETAKWPPAYNDLAKLFSGLAALRRLD
                     LVGAEAGFNAYAGATPGEPAWPYGLQAAARVWKEQVSGFRFQKDSADEWMKNGQGARA
                     RSTWEEFQRKTSPLLYPYVDAEIARAREVEAQEAATRQAEEEKARAEKIQADLDRLDA
                     VRGANAPIVAQKEYRKALANLMQAAPALQTTEGQKALANIREIYERMDELKKFLMTRI
                     NAAPFPGTGTELGGTTIAADTLGIRIALGSHGEMVRTWPEISVRMYMQMLTHYLAAPD
                     ISEADRANLTLSIAVYCYENGGFRAAAGYADQAVKLNPELKVKARILMPDILPD"
     gene            complement(1046..1252)
                     /locus_tag="PK634_10070"
     CDS             complement(1046..1252)
                     /locus_tag="PK634_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98121.1"
                     /translation="MDALAEVVLNGRHEAVEGLIRSAMEHGGAPVTAPLAADLGAGYA
                     KDAAHAVQKLGDMLSRAGKEGNRR"
     gene            complement(1822..1980)
                     /locus_tag="PK634_10075"
                     /pseudo
     CDS             complement(1822..1980)
                     /locus_tag="PK634_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244243.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            2246..3271
                     /gene="queA"
                     /locus_tag="PK634_10080"
     CDS             2246..3271
                     /gene="queA"
                     /locus_tag="PK634_10080"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOW98122.1"
                     /translation="MRTQDFDYELPPDRIAQEPAARRDEARMLVVERETGALHHRRVR
                     DLPEFLRAGDLLVANNTRVIPARVFGVKPTGGKVEILFLEERVPGTWEALMHASRRPK
                     IGDAIRLGSDASAVLLEDGEKGRAVLRVEGAAVPDLLARLGVPPLPPYIHRPARPGLD
                     EERYQTIYAERPGAVAAPTAGLHFTPELLGRLDAAGVPMVFLTLHVGIGTFRPVSADN
                     VEEHRMESERYEVPERTAQAVADTRARGGRIVAVGTTTVRTLEAVAAEHGRVAPCSGR
                     TDLFIRPPFEFKVAGALLTNFHLPRSTLLMLVSAFGGRELVLRAYREAVETGYRFYSY
                     GDCMLLV"
     gene            3342..4085
                     /locus_tag="PK634_10085"
     CDS             3342..4085
                     /locus_tag="PK634_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98123.1"
                     /translation="MTLRPWLNFAASALAQLLVALLFSRLHLIPLHLDFNPGIVLTPL
                     AGILFGAAGVWSALAASLGADWLLGTLGAVSLFQAAGMTAFAWTAWRLWDAAPEPEAS
                     WGRAVFFVTVCVPGALASAAWRALGSELLGLYPYAYIVGVAAVHHMVFCILLGPPLLM
                     AALRFEEPRPAPTSLRRAAAIAGGAVAAALLGVGISRSVYGIGPFEPFVLGLRAGRWV
                     PAAVIPLLLFQLAALAGARGPLWRGRRGA"
     gene            complement(4059..4766)
                     /locus_tag="PK634_10090"
     CDS             complement(4059..4766)
                     /locus_tag="PK634_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HOW98124.1"
                     /translation="MKTRAWMWAGLAIALAGGARTGVAKQAEPVTNLVAALDAAKAGN
                     KLLFVQYGREACGNCQALKGLIRRGQLKLRDTEFVYADVCCDDPATRKAFNDQFKIEG
                     TALPFVIIAAPDGKQLASRSGYGEANEFDRLIDTAEKKFKEEGGPPGAVGGAAPAAGP
                     SRLPMPRPILPDENREVRTWTSRSGSSLEASLVEVTAPYVVLKKADGSKMKILQANLS
                     GGDQAYIEGLRAAPAAP"
     gene            complement(4781..5677)
                     /locus_tag="PK634_10095"
     CDS             complement(4781..5677)
                     /locus_tag="PK634_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98125.1"
                     /translation="MNSLWLSLSLLAMAAACRGDLVAPRGPSVQFLSAFSGGGHLLYF
                     LDHQDQLQEDRFVTEGLMEMDFSMVSFPRGLDIRLRFEMDANMGKSVAENLPFSPKET
                     AYEINPFAEYRREGLLCRAGWVHACQHLIYKDDDSPWYLEEGVDIPPDVYWNRLYAGA
                     GRPEIRPELLRQIFFAEPEGSRRPLAVWYAEAGWYLRSLGGLMDDESLYGDNDWAYDG
                     KLDLMVPLRVTRSIALFAASHAHALFDTNDKAYWRERLELENHFASRGFGSSVFAAWN
                     AVDEHPRDSREGLFELGARFFF"
     gene            complement(5688..6674)
                     /locus_tag="PK634_10100"
     CDS             complement(5688..6674)
                     /locus_tag="PK634_10100"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HOW98126.1"
                     /translation="MRANGIKRFSHAAGALLCLLAAGRAEPFYLPNEHPVTRLFGIPP
                     AESGFIPEGWHARASFNWASISDDDMARGSSLMLDGETYVARLALRRGFADGWEIGLD
                     LPYVRHAGGILDDFIESYHDTFGFAQGNRKTIPSDRLLYALIRNGETLFYLADPVDGP
                     GDVSVSLGRQLYRRPDGTRAVAARAAVEGPTGDRDNLLGSGSTDIALSLTGTDTVLLQ
                     PLRLDAVLGVLFMAGGGLLEDWREPAAGFGSAALEWPATEKLSLKVQADAHSPLFDDL
                     CIGRLDHWGCQVSMGGTLRLPRAAALDIAVTEDVAVATAPDVVFHFALSKSW"
     gene            6778..7446
                     /locus_tag="PK634_10105"
     CDS             6778..7446
                     /locus_tag="PK634_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98127.1"
                     /translation="MSVALPGKLDKRAAGLASALGLLAQTAPATVIDFVPDPEIDTDF
                     LFTPLVSWAPTGPVTQAEAGNGGDLYPGSYSGAVYGCMGNLELSVGTPGGVEYMLSGT
                     YIAPALAINDTVGPGHSQWSGSGSGWQASLDMSGVTWQSPFYVGYRIESGADYLYGYA
                     RLIADFDGGDNLTQIRLTRWAYEDTPNTGITIAAIPEPGLTMLLLIGVAGFLLRRFFR
                     RRAE"
     gene            7689..8357
                     /locus_tag="PK634_10110"
     CDS             7689..8357
                     /locus_tag="PK634_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW98128.1"
                     /translation="MRAQPGKHDKRTAGLASALGLLATTAPATIIRYFPDPIVDTDWH
                     NAPLASWDPMGTDSEAGDGSGLYPGTYAAATYGCSGTMCLSVGTPDGLDFYDGADPDP
                     APRLAVGNTIGPSHTDWTDTDSGYAAYIDNSGYTWGQPFYIGYRIEAGGTDYYYGYAR
                     MSGDYVSGEPAQMRFTVTEWAYENAVNTGITIQPIPEPGMLALLFLGVVALAGRRLFR
                     LRRG"
     gene            8411..10150
                     /locus_tag="PK634_10115"
     CDS             8411..10150
                     /locus_tag="PK634_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98129.1"
                     /translation="MAKACDDARAKSESFLDALEREQSAAPSDPAWPFLRGWLLQSLG
                     RWEEAAGAYADAARLEGPRGAESFYRMGLALARLGLHPPALQAYDRALGRDRGHLGAR
                     LAAGVSLRRLGRVGEALRLLDAYPAPGLPGWVNNHAEFLLEQGRLDEALAELRGAGEL
                     IRRHAGLHSSLIRAMLYDPRVNARDLFEETGRWNAWHAAPAAPSPLREPDPERPLRLG
                     YVSACLHRHNSSAHLANLLRARDRSGFHVTLYSAGTARDDWTAELRALADDWREIAAL
                     SDAEAAERIREDGVDLLVDVNEHANNGRLALFTHKPAPIQFHWYGNAVTTGVKAIDYR
                     FSDAVTEPPGEADRWSHERILRPASGYHCYGPPPGAAEPAPEPPFRRNGFITFGAIHH
                     LAKLNGEVLACWKQILEQVPGSQLLLARDVLADPDTRERLTARLKSTGLNPAAVRLHG
                     DNRTIADLAVYREMDIVLDSFPFNGDATTCDALWMGVPVITLCGERLSARRAAGLLHA
                     VGASEWVASDAESYVRKAVALAADPERLASARAGLPDRVRRSPLCDAAAVTREIEATY
                     RAAWRQACAAVPS"
     gene            10219..11442
                     /locus_tag="PK634_10120"
     CDS             10219..11442
                     /locus_tag="PK634_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOW98130.1"
                     /translation="MAKNSKLTGNMLIAQSGGPTVVINQSLIGAVLEAKKHKEIKKIY
                     GSLHGILGILNENFIDLGKESKATLEAVANTPSSALGSVRKKPTPEDCAKIFQVMQKY
                     DVRYFFYIGGNDSAETTHIINEEARKAGYEFRCFHICKTIDNDLRENDHTPGFGSGAK
                     FVASAFMGDNLDNRALPGVKINVVMGRHAGFLTAASALARVYPDDGPHLVYLPERPFD
                     MDRFLADVKTVNEKYGRCVVAVSEGISDAEGTAIAAKFTKEVDAHGNVQLSGSGALGD
                     LLADQVKAKLQIKRVRADTFGYLQRSFPGVVSAVDAKEARLAGQAAVKFANGGKFANG
                     STAIKRKPGKSYRTWIERTELKNVAKETRHMPDEFINAEGNNVTAAFIRYAAPIVGPL
                     PPVGRFKGVKVKKIG"
     gene            complement(11491..12408)
                     /locus_tag="PK634_10125"
     CDS             complement(11491..12408)
                     /locus_tag="PK634_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOW98131.1"
                     /translation="MALGAAILFWSCVPLLLKYFTRELDAWTVNGLRYTFALLFWLPY
                     VLQHRRELPARSRIWRDAIPPAAIHVVGQVLFGLCPYFNDATVINFVSRAAFLFTTLT
                     GFILLREERAVGRQPLFWIGMAATIAGLVAMYVGGIGTPSTSAFGMLLLLATAAFWGF
                     YSVLVRKFMRPYSIRLSFSVISLYAAPGLLALMFALGDWRKMLTLGPGFWAMIWLSAV
                     LGIALGHTLFYRAIHTIGPVASEGSLLLIPFVTAVLAHFLLDERLGRLQWIGGVILVG
                     GCFVLLRANLRARGRVTAKEAQAAAPTPR"
     gene            complement(12787..13107)
                     /locus_tag="PK634_10130"
     CDS             complement(12787..13107)
                     /locus_tag="PK634_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOW98132.1"
                     /translation="MALSTTPFIPGREIEKSLGVVCGEAILGANVVSDLLAGITDIVG
                     GRSGSYEGKLKAAREIALVELAVEARERGANAVVGIDIDYETLRGSMLMVAASGTAVV
                     AKQT"
     gene            complement(13277..>13695)
                     /locus_tag="PK634_10135"
     CDS             complement(13277..>13695)
                     /locus_tag="PK634_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOW98133.1"
                     /translation="GGTGLSDDVFHALIARGVSVRQFDLATADLGEIAMTLVDTSTIL
                     LGTPAMLNGVHPVAANAAFVINALHPKAKYAGLFGSFGWGSKPLENPAALLPDLQVEL
                     LPTVLARGLPREAEFAALDRLADTVAAKHAAAGLAG"
BASE COUNT         2319 a         4663 c         4555 g         2158 t
ORIGIN      
        1 cccggatgct ggccagtctc gcgctgaacg agaccaccga ggaaaacgtc cggaaggaaa
       61 ccgccaagtg gccgccggcc tacaacgacc tggccaagct cttctccgga ttggcggcct
      121 tgcgccggct ggacctcgtc ggcgcggaag cggggttcaa cgcctacgcc ggggcgacgc
      181 cgggcgagcc cgcctggccc tacggcctcc aggccgccgc acgggtctgg aaggaacagg
      241 tgtccggctt ccggttccag aaggactccg ccgacgagtg gatgaagaac gggcagggcg
      301 cccgggcccg gagcacctgg gaggaattcc agcgcaaaac gagcccgctg ctctatccct
      361 acgtcgacgc ggagatcgcc cgggcccgcg aggtcgaagc ccaggaggcc gcgacccgcc
      421 aggccgagga ggaaaaagcc cgcgcggaaa agatccaggc ggacctcgac cgactcgacg
      481 ccgtccgggg cgccaacgcg ccgatcgtcg cccagaagga ataccgcaag gcgctcgcga
      541 acctgatgca ggcggcgccc gccctgcaga cgacggaggg ccagaaggcc ctcgccaaca
      601 tccgcgagat ctacgagcgg atggatgagc tcaagaagtt cctcatgacc cgcatcaacg
      661 ccgcgccgtt ccccggcacc ggcacggaac tgggcggcac caccatcgcg gccgacaccc
      721 tgggcatccg gatcgcgctc ggcagccacg gggagatggt gcggacctgg cccgagatca
      781 gcgtccggat gtacatgcag atgctcacgc actacctggc ggcccccgac atttcggagg
      841 ccgaccgggc caacctgacg ctgtccatcg ccgtgtactg ctacgagaac ggcgggttcc
      901 gggccgcggc cggttacgcg gaccaggccg tcaagctcaa cccggagctg aaggtcaagg
      961 cgcgcatcct gatgcccgac atactgccgg actgatgccg aacaggagga cgcagagata
     1021 ccggagaagc cctccgctcc ctccgttatc tcctgttccc ctcttttccc gccctggaca
     1081 acatgtcccc cagtttttgc acggcgtgcg ccgcatcctt cgcatagccg gccccgagat
     1141 cggcggcgag tggcgccgtc accggcgcgc cgccgtgttc catcgccgag cggatcaagc
     1201 cctcgaccgc ctcgtggcgg ccgttcaata cgacctcggc cagcgcatcc atggtggtca
     1261 tggaaaatag cacgcgggaa cgacggatgc aaaaaacgaa gcgccggatg accgatgatc
     1321 ctatcggcca tccggcgcct ggcgccgact tatcctctgc tatgggcgca tgacctgatt
     1381 gaccgcatcg ccgacgtaga acgactggtc gttgatcgtg gtattgtcga ggacgtaacc
     1441 cgcgccaccc ttggtcacga acaccatgcc gcgcgtgccg aacggattct cgccgtccgt
     1501 caagttggct tcgctgaccg actggagcgc ggcgatgtcc aggttgcggg taaagatcat
     1561 cgggagggtg tccaccgaag cttccgtgat gtcggccacc atgcaccagg cgttgttatc
     1621 ggcggcaaag ttatcgatat ccttctccga cggcacgccg ccgcccgcga agaacgaagg
     1681 atccacggcc agaacccggt tggtcatcag gttcttccag tagtccgtag agttacccca
     1741 ctccgcgtac gcgggataat cgaacgacaa ctgtccggtc acgacggata cgtcggtgat
     1801 cttttggaag atgccgcgct ggatctgcac gccgttgttc agcatctgcg tcagccgggc
     1861 gcgcagcagc gccccttgga ccgccgggaa gagcagggcg gccaggatgg atatgatggc
     1921 gatgacgacc agcagctcga tcaaagtgaa acctgcaaac cggcgtgaaa cctttttcat
     1981 agttatctcc tacgttcccc ggccctcacc caatgggccc atttctaccg catacggcgg
     2041 ttcgatgtct accttttctt tccttcatcc ttaacgtaca agagttatag aaggtacgtc
     2101 aagagcggtt gcggcggccc caccctcgcc aggtttggag ggcgccgcgt tgtcggcggc
     2161 gcatgggcgg caatgacacg ccgccctccg cgcttcgtgt ttgatccgcg cctcttcctg
     2221 gcctaaaaat cggcgctgca atcttatgcg gacacaagac ttcgattacg agctgccgcc
     2281 ggaccggatc gcccaggagc ccgcggcccg gcgcgacgag gcccggatgc tggtcgtcga
     2341 gcgcgagacc ggggcgctgc accaccggcg cgtccgggat ctgccggagt ttctccgggc
     2401 gggcgacctg ctggtggcga acaatacccg ggtgatcccg gcgcgcgtct tcggcgtgaa
     2461 gccgaccggc ggtaaggtgg aaatcctgtt cctcgaggag cgcgtccctg gtacatggga
     2521 agcgctgatg catgcctcgc gccgcccgaa gatcggggac gcgatccgcc tcgggtccga
     2581 cgcgagcgcc gtgctcctcg aggacggcga aaagggccgc gcggttctgc gcgtggaggg
     2641 cgcggcggtc ccggacctgc tggcccggct gggtgtcccg ccgctgccgc cgtacatcca
     2701 tcggcccgcg cggccgggcc tggatgaaga gcgctaccag acgatctacg ccgagcggcc
     2761 cggcgccgtc gcggcgccga ccgccggcct ccacttcacc cccgaactcc ttggccggct
     2821 cgacgcggcg ggcgtcccca tggtcttcct gacgctgcac gtggggatcg gcacgttccg
     2881 gcccgtgtcg gcggataacg tcgaggagca tcgcatggaa tccgagcggt acgaggtgcc
     2941 ggagcgcacg gcgcaggcgg tcgcggatac ccgcgcccgc ggcggacgga tcgtcgccgt
     3001 gggcaccacg accgtgcgca cgctcgaggc cgtggccgcg gagcacggcc gggtggcccc
     3061 ctgctccgga cgcaccgacc tgttcatccg cccgcccttc gagttcaagg tcgcgggcgc
     3121 gctgctgacg aattttcatc tgccgcgctc gacgctgctg atgctggtca gcgcgttcgg
     3181 ggggcgggaa ctggtgcttc gcgcctaccg cgaggcggtg gaaacggggt accggttcta
     3241 tagttacggg gattgcatgc tgttggtata ggagaaaggg ttcagagttc aggattcagg
     3301 cgtgcctcct gaatcctgcc ccctgaaccc tgtggccctt catgacgctt cgcccatggc
     3361 tgaacttcgc ggccagcgcg ctggcccaac tcctggtcgc gctcctgttt tcccggctcc
     3421 acctgatccc gctgcacctg gacttcaacc cggggatcgt gctcacgccg ctggcgggga
     3481 tcctgttcgg agcggcggga gtgtggagcg cgctggccgc ctcgctgggc gcggattggc
     3541 ttcttgggac actgggggcg gtcagcctgt ttcaggcggc gggcatgacc gcgttcgcat
     3601 ggacggcttg gcggttgtgg gacgcggcgc cggagcccga ggcgtcgtgg gggcgcgcgg
     3661 tgtttttcgt gacggtctgc gtgccgggcg cgctggcctc cgccgcctgg cgcgcgctgg
     3721 gctcggaact gctcgggctg tacccctacg cgtacatcgt cggcgtggcc gccgtgcatc
     3781 acatggtctt ctgcatcctg ctgggcccgc cgctgctgat ggccgcgctg cgcttcgagg
     3841 agccgcggcc cgcgcccacg tccctgcggc gggcggcggc gatcgccggc ggcgcggtgg
     3901 cggcggcgct gctgggcgtc ggcatcagcc gcagcgtgta cgggatcggc ccgttcgagc
     3961 cgttcgtcct cggcctgcgg gccgggcgat gggtgcccgc cgcggtgata ccgctgctgc
     4021 tgttccagtt ggcggcgctg gcaggggcgc gcgggccgct atggcgcggc cggcgcggcg
     4081 cgtagcccct cgatgtacgc ctggtcgccg ccgctcaagt tggcctggag aatcttcatc
     4141 ttgctaccgt ccgccttctt gaggacgaca tagggcgcgg tcacctcgac gagggacgcc
     4201 tcgagcgacg agccgctgcg cgaagtccac gtccgcacct cgcggttttc gtcgggcagg
     4261 ataggccggg gcatgggaag cctcgacggg cccgccgcgg gagccgcgcc cccgaccgcg
     4321 cccggcgggc cgccttcctc cttgaatttc ttctccgccg tgtcgatcag ccggtcgaac
     4381 tcgttggcct cgccgtagcc gctgcggctc gccagctgct tcccgtcggg cgcggcaatg
     4441 atgacgaagg gcagggccgt gccctcgatc ttgaactggt cgttgaacgc cttcctcgtg
     4501 gccgggtcgt cgcagcagac atccgcatag acgaactccg tgtcgcgcag cttcagctgg
     4561 ccgcggcgga tcaggccctt cagcgcctgg caattcccgc acgcctcgcg cccgtactgg
     4621 acgaacagca gcttgttccc ggccttcgcc gcgtcgaggg ccgcgaccag gttcgtgacc
     4681 ggttcggcct gcttggcgac gccggtcctg gcgccgcccg ccagcgcgat cgccagcccc
     4741 gcccacatcc atgcccgcgt tttcatggtg gatctcccgc ttaaaagaag aaccgcgcgc
     4801 ccagttcgaa caacccctcg cggctgtccc gcggatgctc gtccacggcg ttccaggccg
     4861 cgaagaccga cgagccgaag ccgcggctgg cgaaatgatt ctccaactcg agccgctcgc
     4921 gccagtaggc cttgtcgttg gtgtcgaata gcgcgtgcgc gtggctggcg gcgaacagcg
     4981 cgatggagcg cgtcacgcgc aggggaacca tcaggtccag cttcccgtcg tacgcccagt
     5041 cgttgtcgcc gtacagcgat tcgtcgtcca tcagcccgcc gagcgagcgc aggtaccagc
     5101 cggcctcggc gtaccagacg gcaaggggcc gcctactccc ctccggttcc gcgaagaaaa
     5161 tctgccggag aagttcggga cggatctccg ggcggccggc gcccgcgtag aggcggttcc
     5221 agtagacgtc gggagggatg tccacgccct cctcgaggta ccacggggaa tcgtcgtcct
     5281 tgtagatcag atgctggcag gcgtggaccc accccgcgcg gcagagcagg ccctcccgcc
     5341 ggtattccgc gaacgggttg atctcgtagg cggtttcctt gggcgagaag ggcaggttct
     5401 ccgcgaccga cttgcccatg ttggcgtcca tttcgaaccg caaccggatg tcgaggccgc
     5461 ggggaaacga gaccatgctg aaatccattt ccattaggcc ctcggtgacg aagcgatcct
     5521 cctgcagctg gtcctggtga tccaggaaat acaaaagatg cccgccgccg ctgaaggcgg
     5581 agaggaattg gacggatggg cccctggggg cgaccaggtc gccgcggcag gcggcggcca
     5641 tagccaggag ggacagggaa agccagaggg aattcatgcg cattcgttca ccatgatttc
     5701 gaaagtgcga agtggaatac cacatccggc gccgtggcga cagcgacatc ttcggtcacg
     5761 gcgatatcga gtgcggcggc gcggggcagg cgcaacgtgc cgcccatcga gacctggcag
     5821 ccccaatggt ccagccggcc gatgcagagg tcgtcgaaga gcggcgaatg cgcgtccgcc
     5881 tggaccttta acgacagctt ttccgtggcg ggccactcca gcgcggcgga gccgaacccg
     5941 gcggccggct cgcgccagtc ctccagcagc ccgccgccgg ccatgaatag cacgccgagc
     6001 acggcgtcca ggcgcagggg ttggagcagg accgtgtccg tgccggtgag ggagagcgcg
     6061 atatcggtgc tgccgctgcc tagcaggttg tcgcgatcgc cggtcgggcc ttcgaccgcc
     6121 gcgcgggcgg cgaccgcgcg cgtgccgtcc ggccgccggt agagctgtcg ccccaggctc
     6181 acgctcacat cgcccggccc gtccacgggg tccgcgagat agaacaacgt ttccccattc
     6241 cgtatcaggg cgtagagcag gcggtcggag ggaatggttt tccggttacc ttgcgcgaag
     6301 ccgaaggtgt cgtggtagga ctcgatgaaa tcgtcgagga tcccgcccgc gtggcggaca
     6361 tacggtaggt cgagcccgat ttcccaaccg tcagcgaatc cccgccgcag ggccaggcgc
     6421 gcgacgtagg tttcgccgtc gagcatcagc gacgagccgc gagccatgtc gtcgtcggaa
     6481 atgctggccc agttgaagga ggcgcgagcg tgccatcctt ccgggatgaa tccgctctcg
     6541 gcgggcggga tgccgaacag gcgcgtcacg ggatgctcgt tgggcaggta gaacggttcc
     6601 gcccggccgg ccgccagcag gcacagcagg gcgccggcgg cgtgggaaaa gcgttttatg
     6661 ccgttcgctc gcataaccgg gaatcactac cagaatcacg cccgcctgga aagcgccgcg
     6721 cttgacacgc cggcggccgg ggtgctttga taatcatgct tgctcaagga ggattttatg
     6781 agcgtcgcgc tgccgggtaa actcgacaaa cgggccgcag gcttggcgtc cgcgctcggg
     6841 ctgctggcgc agacggcgcc ggccacggtg atcgattttg ttcccgatcc ggagatagac
     6901 acggattttc tctttactcc ccttgtgtcc tgggccccca cgggccccgt gacccaggcc
     6961 gaggccggca acggcggcga cctgtacccg ggctcctact ccggcgccgt gtatggatgc
     7021 atgggcaacc tggaactttc cgtgggcacg ccgggcggcg tcgagtacat gttgagcggc
     7081 acgtacatcg cgccggcgct ggcgatcaat gacaccgtcg gccccggcca ttcacagtgg
     7141 agcggcagcg ggtccggctg gcaggcctcc ctcgacatga gcggtgttac atggcagagc
     7201 ccgttctatg tcggctaccg gatcgagtcc ggcgcggatt acctctacgg ctacgcccgg
     7261 ctgatcgcgg acttcgatgg cggggacaac ctgacccaaa tccgcctcac gcgctgggcc
     7321 tacgaggaca cccccaacac cggcatcacc attgcggcca tccccgagcc cggcttgacg
     7381 atgctcctgc tcatcggggt ggcgggcttc ctgctgcggc gatttttccg gcgccgcgcg
     7441 gaatgacgtt gccgctgcag ccgggccgcc gaacaggcgc gtcacgggat gctcgttgag
     7501 caggtagaag ggttccgccc ggccggccgc cagcaggcac agcagggtgc ttgcgacgtg
     7561 gaagatgcat ttcatgccgg tcgctcgcac agcccggaat caatgccgga atcacgccgg
     7621 cctggaaggc gccgggcttg acagccggac gacgggagtg ctttgatgac tgcaaagggg
     7681 aggggttcat gcgcgcacaa ccgggaaagc acgataaaag aaccgcagga ttggcgtccg
     7741 cgctgggctt gttggccacg acggcgcccg cgaccatcat ccgctatttc cccgacccga
     7801 tcgtggacac ggactggcat aacgccccgc tggcctcttg ggatcccatg ggcacggact
     7861 ccgaagccgg cgacgggagc ggcctgtatc ccggcaccta cgccgccgcg acctacggat
     7921 gttccggcac gatgtgcctg tccgtcggga caccggacgg gctcgatttc tacgacggcg
     7981 ccgacccgga cccggcgccc cggctggcgg tgggcaacac tattggaccg agtcatacgg
     8041 actggaccga caccgacagc gggtacgccg cctatattga caattcgggc tacacgtggg
     8101 ggcagccgtt ctacatcggc taccggatcg aggccggcgg cacggattac tattacgggt
     8161 acgcccgcat gtccggggat tacgtgtcgg gcgagccggc ccagatgcgc ttcacggtca
     8221 ccgagtgggc ttacgagaac gccgtgaaca cgggcatcac catccagccc atccccgagc
     8281 cgggtatgct ggcgttgctg ttcctcggcg ttgtcgcgct ggccgggcgc cgcctcttcc
     8341 gtcttcgccg cggatgacat cgccgctgca ggctgcacgg gcggcgtacc aagccgccga
     8401 ctggccggcc gtggcaaaag cttgtgacga cgcgcgcgcc aagtccgaat cctttttaga
     8461 cgccttggag agggagcagt ccgccgcccc gtccgatccc gcctggccct tcctgcgcgg
     8521 gtggctgttg caatcgctgg gccgctggga ggaggccgcc ggcgcgtacg ccgacgcggc
     8581 gcggctggaa gggccgcgcg gcgccgagtc gttttaccgc atgggcctgg ccctggcccg
     8641 gctcggcctt catcctcccg cgctgcaggc ctatgaccgc gccctcgggc gcgaccgggg
     8701 gcacctaggc gcccggctcg ccgccggcgt ctcgttacgc cggctcggcc gcgtgggcga
     8761 ggccttgcgc ttgttggatg catacccggc ccccgggctc ccgggctggg tcaataatca
     8821 cgccgagttt ctcctggagc agggccggct ggacgaggcc ttggccgaac tgcgcggggc
     8881 cggggaactg atccgccggc acgccggact gcactccagc ctgattcgcg cgatgctgta
     8941 cgatccgcgg gtgaacgccc gcgacctgtt cgaggaaacc ggccgctgga acgcctggca
     9001 cgccgcgccc gcggccccgt cgccactccg cgagcccgac cccgagcgcc ccctgcgtct
     9061 tggttatgtc agcgcctgcc tccaccgcca caacagcagc gcgcacctgg cgaacctcct
     9121 gcgcgcccgg gaccggagcg gcttccacgt caccctgtat tcggccggga ccgcgcggga
     9181 cgactggacc gccgaactgc gcgccctcgc cgatgactgg cgcgagatcg ccgcgctgtc
     9241 cgacgcggag gccgcggaac ggatccgcga ggacggcgtg gacctgctgg tggacgtcaa
     9301 cgagcacgcc aacaacggcc gcctggcgct cttcacgcac aagccggcgc cgatccagtt
     9361 ccactggtac ggcaacgcgg tcacgaccgg cgtgaaagcc atcgactacc gcttctccga
     9421 cgccgtcacc gagccgcccg gcgaggccga ccgctggagc cacgagcgca tcctgcgccc
     9481 ggcatccggc taccattgct acgggccgcc gcccggcgcc gcggagcccg cgcccgaacc
     9541 gccgttccgc cgcaacgggt tcatcacctt cggggccatc catcacctgg ctaagctcaa
     9601 cggcgaggtc ctcgcgtgct ggaagcagat tctggagcaa gtccccggct cgcaactcct
     9661 gctggcccgc gacgtcctgg ccgatcccga tacccgcgag cggttgacgg cccgcctgaa
     9721 atcaaccggc ctcaacccgg ccgccgtccg gctccacggc gacaaccgga cgatcgccga
     9781 tctcgcggtc taccgggaaa tggatatcgt cctcgacagc ttcccgttca acggcgacgc
     9841 caccacctgc gacgccctgt ggatgggcgt cccggtcatc accctgtgcg gcgaacggtt
     9901 gagcgcccgg cgcgccgccg ggctgttgca cgccgtcggg gcgtcagaat gggtcgcgtc
     9961 cgacgcggaa agctatgtgc ggaaagcggt tgcattggcc gcggacccgg agcgcctggc
    10021 atccgcgcgg gccgggctac cggaccgggt gcgccgttcg ccgctctgcg acgcggccgc
    10081 ggtcacccgg gaaatcgagg ccacctaccg ggccgcctgg cgccaggctt gcgccgcagt
    10141 cccctcttga agcggccggc gcggtatggt atctacgggc ttccattccg gaaaaccgtt
    10201 taaccaagag gtgtaagaat ggccaagaac agcaagctga ccggcaacat gctcatcgcc
    10261 cagagcggcg ggccgaccgt agtgatcaac cagagcctga tcggcgcggt gctggaggcg
    10321 aagaagcaca aggaaatcaa gaagatctac ggctcgctcc acggcatcct gggcatcctg
    10381 aacgagaatt tcatcgacct cggcaaggaa tcgaaggcca cgctggaggc cgtcgcgaac
    10441 acgccctcct cggcgctcgg ctcggtccgc aagaagccca cacccgagga ctgcgcgaag
    10501 atcttccagg tcatgcagaa gtacgacgtg cgctacttct tctacatcgg cggcaacgat
    10561 tccgccgaga cgacgcacat catcaacgaa gaggcgcgca aggccggcta cgagttccgc
    10621 tgcttccaca tctgcaagac gatcgataac gacctgcgcg aaaacgacca cacgcccggg
    10681 ttcggcagcg gcgcgaaatt cgtcgcgtcc gcgttcatgg gcgacaacct ggacaaccgc
    10741 gcgctgcccg gcgtgaagat caacgtcgtc atgggccggc atgccggctt cctgaccgcc
    10801 gcctccgcgc tggcccgggt ctatcccgac gacggcccgc acctggtcta cctgcccgag
    10861 cggccgttcg acatggaccg gttcctcgcc gacgtgaaga cggtgaacga gaagtacggg
    10921 cggtgtgtgg tcgcggtctc cgagggcatc tcggacgccg agggcacggc catcgcggcg
    10981 aagttcacca aggaagtgga cgctcacggc aacgtgcagt tgagcggctc cggcgcgctg
    11041 ggcgacctgc tcgccgacca ggtcaaggcc aagctccaga tcaagcgcgt ccgtgccgac
    11101 acgttcggct acctgcagcg ctcgttcccc ggcgtggtgt ccgccgtgga cgcgaaggag
    11161 gcgcgactgg cggggcaggc cgcggtgaag ttcgccaacg gcgggaagtt cgccaacggc
    11221 agcaccgcga tcaagcgcaa gccgggcaag tcgtacagga cctggatcga gcgcacggaa
    11281 ttgaagaatg tggccaagga gacgcgccac atgcccgacg agttcatcaa tgccgagggc
    11341 aacaacgtaa ccgccgcgtt catccgctat gccgccccga tcgtcggccc cctgccgccg
    11401 gtcggccggt tcaaaggcgt gaaggtgaag aagatcggct gatcgccacc ggcggctgaa
    11461 gaaggacgga ctcttgagtc cgtccttttt ttatcgtggc gtcggcgcgg cggcctgcgc
    11521 ctccttcgcc gtgacgcgcc cgcgcgcccg caggttggcc cggagcagga cgaagcagcc
    11581 cccgacgagg atcacgccgc cgatccactg cagccgcccg agccgctcgt ccaggaggaa
    11641 gtgcgccagc accgccgtca cgaacgggat cagcaagagc gatccttccg acgcgaccgg
    11701 gccgatcgtg tgtatcgcgc ggtagaagag cgtgtggccc agggcgatgc ccaagaccgc
    11761 ggagagccag atcatcgccc agaagcctgg cccgagcgtc agcattttcc gccagtcgcc
    11821 gagtgcgaac atcagcgcca gcagcccggg cgcggcgtag aggctgatca cgctgaagct
    11881 caaccggatg gaatagggcc tcatgaactt ccggaccagg accgagtaga agccccagaa
    11941 cgccgccgtg gccaggagca gtagcatgcc gaacgccgac gtggacggcg tgccgatccc
    12001 gccgacgtac atagcgacca gcccggcgat cgtggccgcc atgccgatcc agaacagcgg
    12061 ctggcggccc acggcccgct cctcgcgcag caggatgaag cccgtcagcg tggtgaacag
    12121 gaacgccgcg cggctgacga agttgatgac cgtcgcatcg ttgaagtagg ggcacaggcc
    12181 gaagagtacc tggccgacca cgtggatggc ggcgggcggg atggcgtccc gccaaatgcg
    12241 cgaccgcgcc ggcaactcgc ggcggtgctg caggacgtac ggcagccaga acagcagggc
    12301 gaacgtgtag cgcagcccgt tgacggtcca ggcgtccaac tctcgcgtga agtacttcag
    12361 cagcagcggg acgcacgacc agaacaggat ggccgcgccc agcgccatga gggcgcgcgc
    12421 cagctctctc gaacgactgg gaacaggttc cagcatgcgc gtgaagatgg ggcagccgcg
    12481 gcgggaagtc aatggggcaa gggcgggttc tttcccctca tctacccgac gccaactgtc
    12541 aggacgcctg cgcccccatg catccgcgca acagaatcat aacccgcagt acaatttcca
    12601 cactttgcaa agtgtgaaag tgataatatg tgtaacatat tgtaggatag attgatatac
    12661 gttaatatgc ttgacagata taaactcaca ctttgcaaag tgtgattatg cgaaaaacag
    12721 gctccgcgca tcgcaaagac gcatctcgag gcattatcgg cttgcaccgg cctgctgcag
    12781 gaggcatcag gtctgcttcg ccacaacggc ggtgccggag gcggcgacca tcagcataga
    12841 gccacgcagg gtctcgtagt cgatgtcgat tccgacgacc gcgttggcgc cgcgctcccg
    12901 ggcctctacc gccagttcaa ccagggcgat ctcccgcgcg gccttcagct tgccctcgta
    12961 agagcccgag cggccgccga cgatgtccgt gatgcccgcg aggaggtcgc tgacaacgtt
    13021 cgcgccgagg atggcctcgc cacacacgac accgagcgac ttttcgattt cgcggcccgg
    13081 aatgaacggc gtcgtggaca gcgcaatgtc ccggacgtcg ggatgctcac cccacctctc
    13141 ctggacgatc tccacgccgt tattggcgca cttccagcag gatgtgaact ggtcctcgat
    13201 ctgctccccg cactgacggc atgtccacat aacacccccg ctttcgttag tggcggcaac
    13261 cggccttctc gatccatcag ccggccagtc ccgccgcggc gtgtttcgcc gccacggtgt
    13321 cggccagccg gtccagcgcc gcgaactcgg cctcgcgggg cagcccccga gccagcacgg
    13381 ttggcagcaa ctccacctgg agatcgggca gaagggccgc cgggttctcc agcggcttcg
    13441 agccccagcc gaacgaaccg aacaggcccg catacttcgc cttggggtgc agcgcgttga
    13501 tcacgaacgc cgcgttcgcg gccacgggat gcacgccgtt gagcatcgcg ggagtgccga
    13561 gcaggatcgt ggacgtgtcc acgagcgtca tcgcgatctc acccaggtcc gccgtggcca
    13621 ggtcgaactg ccgcaccgag accccgcgcg cgatcagcgc gtggaagacg tcgtcggaca
    13681 ggcccgtgcc gccgt
//
LOCUS       DAOOTK010000090        13613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3023_length_13613_cov_5.133132, whole genome shotgun sequence.
ACCESSION   DAOOTK010000090 DAOOTK010000000
VERSION     DAOOTK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3023_length_13613_cov_5.133132"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK634_10140"
     CDS             <1..878
                     /locus_tag="PK634_10140"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOW98134.1"
                     /translation="NPFLEMADMVVAETTCDGKKKMFELMAGTRPLHLLELPQKPDEP
                     DALGRWMAEYIKLRRVLEHRFGVRITDERLREETRRMNRERKLKRDLAALMKADEPPL
                     TGRELLDMKSLIACIPADLDQYEQALTELPGRKLDPPAGGRLRVLLTGVPLPHGAERV
                     MDVIEGHGGLVVCQENCTGLKPILDDVDADAPDILRALAEKYLRLPCSIMTPNARRLD
                     SLRRLAAEYRAECVVELVWQACLTYDVEAHRVRRLATEELGLPYLRIETDYSPSDSAR
                     IAVRIQALFETARRA"
     gene            875..1705
                     /locus_tag="PK634_10145"
     CDS             875..1705
                     /locus_tag="PK634_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOW98135.1"
                     /translation="MITAGIDAGSRMIKVVLWDAGRGAIHASAQVEQGVQQAQRARDA
                     FDEALRRADLAPGDIARTVATGYGRNAIEWADTTLTEITCHARGVRRQTPEARTVIDI
                     GGQDSKLIRLDADGRVRDFMMNDRCAAGTGRFLEIVAQRLELPLNELGAAAGRAQRPS
                     VISSMCVVFAETEIVGLLSAGERPEDIVAGVQAAMASRVASMAGHRLDEPAVFTGGVA
                     LIPGMDRALADALGRSVRVAPDPRLTGALGAALLAAEEWSRCLPQRTQRDTEGMPSIP
                     "
     gene            1793..2209
                     /gene="nikR"
                     /locus_tag="PK634_10150"
     CDS             1793..2209
                     /gene="nikR"
                     /locus_tag="PK634_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOW98136.1"
                     /translation="MPTVTRFSVSLSNELLEQFDAQIKREGCPTRSKAVGDLIRKSLV
                     EIEWHEGGEVAGAIVLVYDHHKPDLVRKLTGAQHDCHDAIISTQHIHLDHDNCLEIVA
                     VRGKPRMISAIVQRLKAVKGLKHVSLAAGTTGRRLA"
     gene            2326..3060
                     /locus_tag="PK634_10155"
     CDS             2326..3060
                     /locus_tag="PK634_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOW98137.1"
                     /translation="MTTRLLVVPAVAFLFVAPALAGRPLSVDDADPVAPGLIEAEAGA
                     GYTKEGGIKAWDMPVGLTYGVLEGLEAGIGFGGQYVEAGGEDESGIRDTAIGAKWQFL
                     PSCPLGARHALAPSVKLPTADEDKGLGSGETDFDLTWIISRTLGEKAGAHVNLGYAWI
                     GGPEDDVLHYGFALDYQATETMQAVGEVFAERETSGGADTVGQFNLGLRYSATDALAL
                     DLAAGSRIGDDGPDFTVTAGLTWVLE"
     gene            3153..4172
                     /locus_tag="PK634_10160"
     CDS             3153..4172
                     /locus_tag="PK634_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOW98138.1"
                     /translation="MHMADALISPAVGGTMWAASAGLLAWCARKIRQTLDHRLVPMMG
                     VLGAFIFASQMINFSIPGTGSSGHLGGGLILAILLGPHAAFLVIASVLTVQALFFADG
                     GLLALGCNIWNLGFYPAFIAFPFIYKPLAGANRATPRAWAAAILAAVIGLQLGAFSVV
                     LETTASGISELPFGSFVLLMSPVHLAIGIVEGLATATVVAFVARARPEAVPSPAGSAS
                     LKPALIGLGIAALIVGGAVSWFASAHPDGLEWSIAKVSGREEVGGGAALHEKLARVQE
                     KTAFLPDYGFQPAEEPAAEGEPGPEPWPAVSAGTTVSGVVGGLLVLLVAGAAGLALRP
                     RRVAS"
     gene            4181..4987
                     /gene="cbiQ"
                     /locus_tag="PK634_10165"
     CDS             4181..4987
                     /gene="cbiQ"
                     /locus_tag="PK634_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOW98139.1"
                     /translation="MAMAHGGTVQRMDELAALESPAHRLDARAKAVVTFAFLVAVMSF
                     PRHEVVALAPYVLYPVALLSLGGIPARWILKKLLVAAPFALFIGLFNPLLDRQPVALP
                     GGLFVAAGWLSFASILARFALTVSAALALVACTGMDRLCAGLERLGLPRAFAVQLLFL
                     HRYLFVISDEARRMRYGLALRTADGRAPSLRVYGSLLGHLLLRSMDRAQRIYGAMLAR
                     GFDGEIRILGTARWTARDALFTTGWLAFFAAARFWNLPEFAGRLLTGELR"
     gene            4984..5727
                     /locus_tag="PK634_10170"
     CDS             4984..5727
                     /locus_tag="PK634_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW98140.1"
                     /translation="MSHHIVEARDVHYTYPGGHEALRGVSFRITHGEAVAVVGPNGAG
                     KSTLLQHLNGTLLPARGEVRIGDDPVTRDTAPRIRRAVGMVFQDPDDQLFMPTVLEDV
                     AFGPLNHGLAPEAAAERARAALERVGMSHAADQPPYRLSAGEKRAVSIAGVLALSPDV
                     LVMDEPSSNLDPRGRRRLMELLKSFEHTKIIATHDLELVVEVCSRVILLDGGLVKAEG
                     PVVQVLNDEPLMLAHGLERPHSLRHRHPH"
     gene            complement(5792..6469)
                     /locus_tag="PK634_10175"
     CDS             complement(5792..6469)
                     /locus_tag="PK634_10175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOW98141.1"
                     /translation="MCEQPRSLPLIGEKAPSFKARTTQGEIDFPKCYEGKWVVFFSHP
                     ADFTPVCTTEFMTFASMQAEFEKRNCKLLGLSIDSIYAHIAWLRTIKEKIQYKDMKGV
                     EVTFPVIEDLKMEVSKAFGMLQPGASTTQAVRAVFLIDPKGIVRAILYYPLSNGRNMD
                     EVMRLLIAMQTSDQHGIATPANWKPGDDVIVPPPGSCGVAKDRVEKADPDVKCLDWFM
                     CLKKCPK"
     gene            complement(6488..6994)
                     /locus_tag="PK634_10180"
     CDS             complement(6488..6994)
                     /locus_tag="PK634_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOW98142.1"
                     /translation="MGTKGIEIVDMDVRKLVGLLNKAFSDEWLAYFQYWIGAKVVKGP
                     MKEAVIAELTQHAADELRHADMLALRIIQLGGTPVNEPKEWYRHSNCGYEAPADPFVR
                     EVLEQNIKGEQCAIVTYKNLVNLTMGKDPVTYNIVLQILQDEVEHEEDLQAEMEDLQT
                     LLKSGAKK"
     gene            complement(7207..7665)
                     /locus_tag="PK634_10185"
     CDS             complement(7207..7665)
                     /locus_tag="PK634_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOW98143.1"
                     /translation="MPLDPVSVASRVEAFAAACRRAGLKTTPQRAEIYRELARTEEHP
                     DAETLYRRVRKRLPSLSLDTVYRTLRMLEGRGIVSRMGTIRERARYDANTERHHHFVC
                     TRCGLVGDFHCEALDTFPPPVEAGTMGRVDSIHVELRGVCRACQQKRKRG"
     gene            7788..9311
                     /locus_tag="PK634_10190"
     CDS             7788..9311
                     /locus_tag="PK634_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOW98144.1"
                     /translation="MVRKLIRRVLHSLLGRKKKPAESAPPAPPAPHAKGHKPPDRQPP
                     RPRDRQGPRPHEPRSHAPRRAPPAPWDPASFDVPALEGKTRFQDLDLPVEILHAVADL
                     DFKYCTPIQAAILPPTLAGKDAFGRAQTGTGKTAAFLVAVFTRFLRRPAKEPRPKGTP
                     RALVIAPTRELVIQIEKDARELARYLPLRVLAVYGGMDYDKQRRELTSGSVDLVAATP
                     GRLLDFKRRGDIHLDQVEVLVIDEADRMLDMGFIPDVRTIVHSTPPKSERQTLFFSAT
                     LTPEVTRLASQWTTDPVHIEIAPEQVAVDSVDQRVYIVTLRDKFALLYHILNRENATR
                     VLVFANRRDQSERLMENLRRYGIDCALLTGAVDQRKRLRVLEDFREGKIRVVVATDVA
                     GRGLHVEGISHVINYNLPMDPEDYVHRIGRTGRAGASGISICFADEDDGHYLPQVEKF
                     IGRTLSCTHPDDDWLVLPPPLYEAREPKYSSRRPDRPGRSFGPRRGGGPRRGGRRTH"
     gene            complement(9318..10043)
                     /locus_tag="PK634_10195"
     CDS             complement(9318..10043)
                     /locus_tag="PK634_10195"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOW98145.1"
                     /translation="MKHGHGTAGRWAAIGLAMAFGAGLAPAGEMRTWTSAGGSTMQAE
                     FVEQRLDQVILRNAEGKTIQIQMNRLSREDQIYVGSLRTAAMQAAPAGASAETPIPPA
                     IEALFGTRLVNAKGQRVSPAVLDGKKIGIYFSAHWCPPCRAFTPQLVTVYNELRKAGK
                     PFEIVFVSSDQTEADMYRYMKDMEMPWTAVRFPDKNRDELKKKYNIQGIPTLVIIDSS
                     GKTISANARSDVANKGAAAFDAW"
     gene            10195..10533
                     /locus_tag="PK634_10200"
     CDS             10195..10533
                     /locus_tag="PK634_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOW98146.1"
                     /translation="MDYLENWIVQARKGFLELCILNALEGEERYGYDLVKSLVDTPGL
                     GLTEGTLYPLLSRLRLQGLVTTRLEESPEGPARKYYALTARGRKIAKQMNEHVEQMLQ
                     GSRGLRARKE"
     gene            10537..13152
                     /locus_tag="PK634_10205"
     CDS             10537..13152
                     /locus_tag="PK634_10205"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOW98147.1"
                     /translation="MLEWTVSAEAELERYLAEARTAASVSGADPEEVEGDLRRHIEQH
                     LADAGVRRATRDDLARVLSRLRPARPTPASEPPEAIAKPRRISLGLIMAFGVVLPVIT
                     LVFEAFTGLCGELFVNPIPTLWHVLLVATVPLANALAWSRLAFGRPASLKLVGLFNGF
                     AIGVALYYTFVFLPVTPFAAIGIIYFGIGLLPLAPMLSLIMALAARRILRRYDRPVGA
                     LPPAGRWALAALGTIALLATPTIVTQAGLQWATTESPATRARGVGLLRALGNDDLLLR
                     SCYRRDRAVGNMVSFLFEGLGRRITSEQAQQVYYRVTGTPYNAVRPPELRGLRTRALI
                     NAEDFDWDQGGDAVAGRLRGLSLRESRLDSRIEADSGAAYTEWILVFRNDAERQREAR
                     AQVALPPGAVVSRVTLWVDGEEREAAYAGRAKVKAAYQRVVQQRRDPVLVTTSGPDQV
                     LVQCFPVPANGGTMKIKLGITAPLAPESREAGLLRLPYFRERNFSVPREVKHSAWIES
                     PQTVAALQPEDQVLAEHPSERVYALRAELEDRALSRPFAVRVAMAGHPAWAALREDES
                     RVVRQVLDETPVDKPGRIAFVFDGSRSMSSEAAAFGEVLKYLPAGVETAVLLAGDRPL
                     TVRETAPWAPEAATDVVKALSEARYRGGCDNVSALAEAWDLAAAVPGGTVVWIHGPQP
                     VSFEGEEALLQRIERRPNGPVILDLQVGAGPNAVAVMLNGSPALRAVADFGDPAAALA
                     RILDAWAGRKAFYRFERTRVPRSDIAGDIPEGSSHLARLWAFSEILRLGASIHEADKE
                     EAVALAVRYRLVTPVSGAVVLETAKQYQESGLEPAAGDTVPRVVPEPQTWMLLLMGGG
                     LAAARAWKRRRARPS"
     gene            complement(13162..13488)
                     /locus_tag="PK634_10210"
     CDS             complement(13162..13488)
                     /locus_tag="PK634_10210"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HOW98148.1"
                     /translation="MEEGVRQTAESAALGWALLALAASHLFSFVTNYLAGGECRRADV
                     GRLMFQPYGRVVVLHLAILGGAFLLQQFGSPAAGLALLVLLKTALDALAHLWERSKFS
                     PASVAA"
BASE COUNT         2063 a         4731 c         4560 g         2259 t
ORIGIN      
        1 caaacccatt ccttgagatg gcggacatgg tcgtcgcgga gaccacctgc gacgggaaga
       61 agaagatgtt cgagctcatg gcggggacgc ggccgctcca cctgctcgaa ctgccgcaga
      121 aaccggacga gcccgatgcg ctgggccgct ggatggcgga atacatcaag ttgcgacggg
      181 tgctggagca tcgtttcggg gtccgcatca ccgacgaacg gctgcgcgag gagacgcgcc
      241 ggatgaaccg cgagcggaaa ctgaagcgcg atctcgccgc cctgatgaag gccgacgaac
      301 ctccgctgac cggacgcgaa ttgctggaca tgaagagcct gatcgcctgc atcccggccg
      361 acctggacca gtacgagcag gccctgacgg aactgccggg ccggaaactc gacccaccgg
      421 ccggcggccg cctccgtgtg ctgctgaccg gcgtgcccct gccgcacggc gcggaacggg
      481 tgatggacgt gatcgaggga catggcggcc tggtcgtctg ccaggagaac tgcaccgggc
      541 tcaagccgat tctcgacgat gtcgacgcgg atgcgccgga tatcctgcgc gccctggcgg
      601 agaagtacct gcgcctcccc tgctccatca tgacgccgaa cgcgcgccgc ctggattcgc
      661 tgcgccggct cgcggccgag taccgggcgg aatgcgtggt cgaactggtc tggcaggcct
      721 gcctgaccta cgacgtcgag gcgcaccgtg ttcgacggct cgcgaccgag gaactggggc
      781 ttccatacct gcgcatcgag accgactact ctccgtccga ctccgcccgg atcgccgtac
      841 ggatccaagc gctcttcgaa acagcccggc gcgcatgatc acggccggga tagacgcggg
      901 ttcgcggatg atcaaggtgg tcctgtggga cgccgggagg ggcgccatcc acgcctccgc
      961 gcaggtcgag cagggcgtgc aacaggcgca gcgggcgcgc gacgcgttcg acgaggccct
     1021 gcgccgggcg gacctggccc ccggcgacat cgcgcgcacc gtcgccacgg gctacggacg
     1081 caacgccatc gagtgggccg acacgaccct gacggagatc acgtgccacg cccgcggcgt
     1141 gcggcgccag acacccgagg cgcggacggt gatcgatatc ggcggacagg acagcaagct
     1201 gatccggctc gacgccgacg ggcgggtccg ggacttcatg atgaacgacc ggtgcgccgc
     1261 gggaacgggc cgcttcctgg agatcgtcgc gcagcggctg gagctgccgc tcaatgaact
     1321 cggcgccgcg gccggccggg cgcagcggcc ctccgtcatc agcagcatgt gcgtggtctt
     1381 cgccgagacg gagatcgtcg ggttgctctc cgcgggcgag cggccggaag acattgtcgc
     1441 gggtgtccag gccgcgatgg cctcgcgggt cgcctcgatg gcgggccacc ggctggacga
     1501 accggcggtc ttcacgggcg gggtggcgct gatccccggc atggaccggg cgctggcgga
     1561 cgccctgggc cgttcggtcc gggtcgcccc cgatccgcgc ctgaccggcg cccttggcgc
     1621 ggcgctgctg gccgcggagg agtggagccg ttgtttaccg cagaggacgc agagggacac
     1681 agagggaatg ccttctattc cctgattctc cgcggccttc gtgccctcgg cggttcaacc
     1741 cctttcttgc ctggccggat tctcctgata aagtgcccac ggagacagga tcatgcccac
     1801 ggttacccgg ttcagcgtgt cgttgagcaa cgagttgctg gagcagttcg acgcccagat
     1861 caagcgcgag gggtgcccca cccgctccaa ggccgtcggc gacctgatcc ggaagagcct
     1921 ggtcgagata gagtggcacg agggcggcga ggtcgcgggg gcgatcgtcc tggtctacga
     1981 ccaccacaag ccggaccttg tccggaagct caccggcgcc cagcacgact gccatgacgc
     2041 gatcatttcg acccagcaca tccacctcga ccacgacaac tgcctggaga tcgtcgccgt
     2101 ccggggcaag ccccgaatga tctcggccat cgtgcaacgg ctcaaggcgg tcaagggtct
     2161 caagcacgtc tccctcgccg ccggcaccac cggccggcgg ctggcctgac ggccgcgccg
     2221 ctccttcccc ttttttctct tgcgctcgcc cgcagttgtg ttacgttttt tgcattcgta
     2281 ttaactcgcc gggcgtggtc cggcgcaacc ctgaaggaaa accacatgac gactcgcctg
     2341 ctggttgtac ccgctgtcgc cttccttttt gtcgcgccgg ccctggccgg aaggcccctg
     2401 tccgtcgacg acgcggatcc cgtggccccc ggcctcatcg aggccgaggc gggcgccggc
     2461 tacaccaagg aaggcggcat caaggcctgg gacatgccgg tcggcctgac ctacggcgtc
     2521 ctggagggcc tggaagccgg gatcggattc gggggccagt acgtcgaggc gggcggcgag
     2581 gacgaatccg gcatccgcga cacggcgatc ggcgcgaagt ggcagttcct gccctcgtgc
     2641 cccctcggtg cgcgccacgc gctcgcgccg tcggtgaagc tccccacggc cgacgaggac
     2701 aagggcctcg gcagcggcga aacggatttc gacctgacct ggatcatctc gcggacgctc
     2761 ggggagaagg ccggcgcgca cgtcaatctg gggtacgcct ggatcggcgg cccggaagac
     2821 gacgtgctcc attacggctt cgcgctggat taccaggcga ccgagaccat gcaggccgtc
     2881 ggcgaggtct tcgcggaaag ggaaacgtcg ggcggcgcag ataccgtggg ccagttcaac
     2941 ctcgggctgc gctacagcgc cacggacgcg ctggccctgg acctggccgc cggctcgcgg
     3001 atcggcgacg acgggccgga tttcaccgtc acggcgggcc tgacctgggt gttggaatga
     3061 tggaccggag ggcgccgcgt tgtcggcgcc ctatcaagcg gcgatgacac gccgccctcc
     3121 ggaagagcaa tacgcgaaag gaagataaag acatgcacat ggccgatgcg ttgatttccc
     3181 ccgccgtggg cgggacgatg tgggccgcaa gcgccgggct gctggcctgg tgcgcgcgca
     3241 agatccggca gacgctggac caccggctgg tcccgatgat gggcgtgctg ggcgcgttca
     3301 ttttcgcctc gcagatgatc aacttttcga tcccgggcac gggttcgagc ggccacctcg
     3361 gcggcgggct gatcctggcg atcctgctcg gcccgcacgc ggcgttcctc gtcatcgcgt
     3421 ccgtactgac cgtgcaggcg ctgttcttcg cggacggcgg cctgctggcg ctgggctgca
     3481 acatctggaa cctgggcttc tacccggcct tcatcgcttt tccgttcatt tataagccgc
     3541 tggccggcgc caaccgcgcg acgccgcgcg cctgggcggc cgcaatcctg gccgccgtga
     3601 tcgggctgca gctcggcgcg ttcagcgtgg tgctggaaac aaccgcctcg ggcatctctg
     3661 agctgccgtt cggctcgttc gtgctgctga tgtcgcccgt ccaccttgcc atcgggattg
     3721 tggagggatt ggcgacggcg acggtggtcg ctttcgtcgc ccgggcgcgg ccggaggccg
     3781 tcccgtcgcc ggccggctcg gccagcctca aacccgcctt gatcgggttg ggcatcgccg
     3841 cgttgatcgt gggcggcgcc gttagctggt tcgcctccgc gcacccggac ggcctggaat
     3901 ggtccatcgc gaaggtcagc ggccgcgaag aggtcggcgg cggcgcggcc ctgcatgaaa
     3961 aactggcccg ggtccaggag aaaacggcct tcctgcccga ttacggtttc cagcccgcgg
     4021 aggaaccggc cgcggaaggc gagccgggcc ctgaaccctg gcccgcggtc agcgccggga
     4081 caacggtttc gggcgtcgtg ggcggtctgc tcgtgctgct cgtggccggc gcggcggggc
     4141 ttgcgctgcg cccgcggcgc gtggcatcct gaattatctt atggccatgg cacacggcgg
     4201 gacggtccag cgcatggatg aactggccgc cttggagtcg cccgcgcatc gcctggatgc
     4261 gcgcgcgaaa gccgtggtca cgtttgcgtt ccttgtcgcc gtgatgtcgt ttccccggca
     4321 cgaggtcgtc gctctggccc cctacgtcct ctacccggtc gcgctgctgt cgctcggggg
     4381 gatccccgcg cgctggatcc tgaagaagct cctggtcgcc gcgccgttcg cgctgttcat
     4441 cggcctgttc aaccccctgc tcgaccggca gccggtggcg ctgcccggcg ggctcttcgt
     4501 ggccgccggc tggctgtcct tcgcctccat tctcgcgcgc ttcgcgttga cggtcagcgc
     4561 ggcgctggcg ctcgtggcct gcacgggcat ggaccggctt tgcgcgggcc tggagcggct
     4621 gggcctgccg cgtgccttcg ccgtccagct gctgttcctc caccggtatc ttttcgtcat
     4681 ttcggacgag gctcggcgga tgcggtacgg gctggcgctg cggaccgccg acggccgggc
     4741 cccatccctg cgcgtctacg gctcgctgct cggccacctg ctcctgcggt ccatggaccg
     4801 ggcgcagcgg atctacggcg ccatgctcgc gcgcggcttc gacggggaga tccgcatcct
     4861 gggaacagcg cgctggacgg cgcgcgacgc gctcttcacc acgggctggc tcgccttctt
     4921 cgccgccgcc cgattctgga acctgcctga gttcgcgggc cgcctgctga ccggagagct
     4981 acgatgagcc atcacatcgt cgaggcgcgg gacgtccact acacctatcc cggcgggcac
     5041 gaggccttgc ggggcgtatc gttccggatc acgcatggcg aggccgtggc cgttgtgggc
     5101 cccaacggcg cgggaaaatc cacgctgctg cagcacctca acggcacgct gctgcccgcg
     5161 cgcggagagg tccggatcgg ggacgacccg gtcacgcggg ataccgcgcc ccggattcgc
     5221 cgcgcggtgg gcatggtctt ccaggacccg gacgaccagc tgttcatgcc gaccgtgctc
     5281 gaggacgttg cgttcggccc gctgaaccac gggctcgcgc cggaagccgc cgcggagcgg
     5341 gcccgcgcgg ccctggagcg ggtgggcatg agccacgcgg ccgaccagcc gccctaccgc
     5401 ctgtcagccg gggagaagcg ggcggtctcc atcgcgggcg tgctggccct gtcgccggac
     5461 gtgctggtga tggacgaacc gtcgtccaac ctcgatccgc gcggacggcg ccggctcatg
     5521 gagctgctga aatccttcga gcacacgaag atcatcgcca cgcacgacct cgaactcgtc
     5581 gtcgaggtct gttcccgcgt catcctgttg gacggcgggc tcgtgaaagc cgaagggccc
     5641 gtcgtccagg ttctgaacga cgagcccctc atgctggccc acggccttga gcggccgcac
     5701 agcctgcggc accggcaccc gcattgaatc cgcctccgcg caaggctacg gcggacaagc
     5761 cgggcccgcg ggccacacca cgccgattat tttacttcgg gcactttttc aggcacatga
     5821 accaatccag gcatttcacg tccggatcgg ccttctccac gcgatccttg gccacgccgc
     5881 aggagcccgg cggcggcacg atgacgtcgt cacccggctt ccagttcgcc ggcgtggcga
     5941 tgccgtgctg gtccgaggtc tgcatcgcga tcagcaggcg catgacctcg tccatgttcc
     6001 ggccgttcga gagcgggtag tagaggatcg cccgcacgat gcccttggga tcgatgagga
     6061 acaccgcgcg gacggcctgc gtggtgctcg cgcccggctg cagcatgccg aaggccttcg
     6121 agacctccat cttcaggtcc tcgatcaccg ggaaagtgac ctcgacgcct ttcatatcct
     6181 tgtactggat cttctccttg atcgtccgca gccacgcgat gtgggcgtag atgctgtcga
     6241 tggacaggcc cagcagcttg cagttgcgtt tctcgaactc cgcctgcatg gaggcgaacg
     6301 tcatgaactc cgtcgtgcac accggcgtga aatccgccgg gtgcgagaag aacacgaccc
     6361 acttgccctc gtaacacttg gggaaatcga tctccccctg cgtcgtcctg gccttgaacg
     6421 acggcgcttt ttccccgatc aggggcagac tcctcggttg ctcgcacata ggcactctcc
     6481 tccgtggtta cttttttgct cccgacttca ataacgtctg caggtcctcc atctcggcct
     6541 ggaggtcctc ctcgtgctcc acctcatcct gcaggatctg gaggacgatg ttgtacgtca
     6601 cggggtcctt gcccatcgtg aggttaacga gattcttgta ggtgacgatc gcgcactgct
     6661 cgcccttgat gttctgctcc aggacctccc ggacgaaggg atccgccggc gcctcgtagc
     6721 cgcagttgga atgcctgtac cactccttcg gctcgttcac cggcgtgccg ccgagttgga
     6781 tgatgcgcag cgcgagcatg tccgcgtgtc gcagctcgtc cgccgcgtgc tgggtcagct
     6841 cggcgatgac ggcctccttc atcgggccct tcaccacctt cgcgccgatc cagtactgga
     6901 agtacgccag ccactcgtcc gagaaggcct tgttcagcag gccgacgagt ttcctgacgt
     6961 ccatatccac gatctcaatg cctttggtgc ccatgatgct cccttccttt tcttgtgctt
     7021 gaaccccgac gccgggaggg ccgcccggaa gcgactcctt cccacgccgg cagattatac
     7081 tacggaggcg gccctttcga gaccaaaggc gactcgcccg ccgcatccca cggctcccgg
     7141 cctggccgtc ttaatcagta ataggtattt tttagttatt attactatat tagggcaaga
     7201 aaaaaactac cctcgtttcc gcttctgctg gcaggcccgg cacacgccgc gcagttcgac
     7261 gtggatggaa tccacccggc ccatcgtccc cgcttcgacc ggcggcggga acgtgtccag
     7321 cgcctcgcag tggaagtcgc cgacgagccc acagcgcgtg cagacaaaat gatgatggcg
     7381 ctccgtgttg gcgtcgtacc gcgcgcgctc ccggatcgtg cccatgcgcg agacgattcc
     7441 ccgcccttcc agcatccgca gcgtccggta caccgtgtcc agggagaggg acggcagccg
     7501 cttccgcacc cggcggtaca gcgtctcggc gtcgggatgc tcctccgtgc gcgcgagttc
     7561 gcggtagatc tccgcgcgct ggggggtggt cttgaggcct gcgcggcggc aggccgcggc
     7621 aaaggcttcc acgcgcgagg ccacgctgac cggatcaagg ggcatgcatc atcaagtaat
     7681 aattattact tgttggaaag atagggttcc gcggccccgg tgtaaagggc tttccccgcc
     7741 aggcttggct tgaccgcggg aggccccgct gtcatgatgc tgctctcatg gtccgcaaac
     7801 tcattcgacg cgtcctgcac tccctcctgg gaaggaagaa gaagccggct gaatccgcgc
     7861 cccccgcgcc gccggcgccc cacgccaagg gacataagcc ccccgaccgg caaccgccca
     7921 ggccccggga tcgccagggg ccgcgtcccc acgaaccgcg atcccacgcg ccacggcgcg
     7981 ctccgccggc gccctgggac ccggcctcct tcgacgtccc cgcgctggaa ggaaagaccc
     8041 ggttccagga cctggacctg cccgtggaaa tcctccacgc ggtcgccgac ctcgatttca
     8101 agtactgcac gcccatccag gcggccatcc ttccccctac gctcgcgggc aaggacgcct
     8161 tcggccgcgc ccagacgggc acgggcaaga ccgccgcctt cctggtcgcc gtgttcaccc
     8221 ggttcctgcg ccggcccgcg aaggagcccc ggcccaaggg cacgccgcgc gcgctggtca
     8281 tcgccccgac gcgcgaactg gtgatccaga tcgagaagga cgcccgcgaa ctggcccgct
     8341 acctgccctt gcgcgtcctg gccgtgtacg gcggcatgga ctacgacaag cagcgccgtg
     8401 aattgaccag cggctccgtc gacctggtcg cggccacgcc cggccgcctt ctcgatttca
     8461 agcgccgggg cgatatccat ctcgaccagg tcgaagtcct ggtcatcgac gaggccgacc
     8521 ggatgttgga catgggcttc atcccggacg tccgcacgat cgtccacagc acgccgccca
     8581 agagcgagcg ccagaccctg ttcttctccg ccacgctgac gccggaggtc acgcggctcg
     8641 cttcccagtg gaccaccgac ccggtccaca tcgagatcgc tcccgagcag gtcgcggtgg
     8701 acagcgtgga ccagcgggtc tacatcgtca cgctccgcga caagttcgcc ctgctctacc
     8761 acatcctgaa ccgcgagaac gccacgcgcg tgctggtgtt cgccaaccgc cgggaccagt
     8821 ccgagcggct catggagaac ctccggcgct acggcatcga ctgcgccctg ctcaccggcg
     8881 cggtggacca gcggaagcgg ctgcgggtgc tcgaggattt ccgcgagggc aaaatccggg
     8941 tggtcgtcgc caccgacgtc gcgggccgcg gcctgcacgt cgagggcatc agccacgtga
     9001 tcaactacaa cctgccgatg gacccagagg attacgtcca tcgcatcggt cgcacaggcc
     9061 gcgcgggcgc cagcggcatc tcgatctgct tcgcggacga ggacgacggg cactacctgc
     9121 cgcaggtgga gaaattcatc gggcggaccc tttcgtgcac gcacccggac gacgactggc
     9181 tggtcctgcc gccgccgctc tacgaggccc gggaaccgaa gtattcctcg cgccggccgg
     9241 accggccggg ccggtccttc ggcccccggc ggggcggcgg cccgcgccgc ggcggccgac
     9301 ggacgcattg acggagttca ccacgcgtcg aaggccgcgg cgcccttgtt cgccacgtcg
     9361 ctgcgcgcgt tggccgaaat cgtcttgccg ctggaatcga tgatcaccag ggtcgggatg
     9421 ccctggatat tgtacttctt cttgagctcg tcccggttct tgtcggggaa gcgcaccgcg
     9481 gtccagggca tttccatatc cttcatgtag cggtacatgt ccgcctcggt ctggtcgctg
     9541 gacacgaaga cgatctcgaa cggtttgccc gccttccgga gttcgttgta gacggtgacg
     9601 agctggggcg tgaacgcccg gcacggcggg caccagtgcg cggagaagta gatgccgatc
     9661 ttcttgccgt cgagaacggc cggcgacacc cgctgcccct tggcgttcac cagacgcgtg
     9721 ccgaagaggg cctcgatcgc ggggggaatg ggcgtctcgg ccgaggcgcc cgcgggggcc
     9781 gcctgcatgg ccgcggtccg cagcgagccg acgtatatct ggtcctcgcg gctcaaccga
     9841 ttcatctgga tctggatggt cttgccttcc gcgttgcgga ggatcacctg gtccaggcgc
     9901 tgctcgacga actccgcctg catcgtcgag ccgcccgcgc tggtccacgt gcgcatctcg
     9961 ccggccgggg cgagtccggc cccgaacgcc atcgccaagc cgatcgccgc ccatcgcccc
    10021 gccgttccat gcccgtgctt catgtccttc ctccgtctcc cgaatgggcc cgacgatagg
    10081 cgcgccgcgc ccggaattca acgggaatcg cggcgggagg gccgccctac aaatatatct
    10141 tgcatgacca tgtaccttac tatacaaggt agcgtgacgg acaggatggg tctcatggac
    10201 taccttgaaa actggatcgt gcaggcccga aagggcttcc tggagctgtg cattctcaat
    10261 gcgctcgaag gcgaggagcg ctacggctac gacctggtga agtcgctcgt cgatacgccc
    10321 ggcctcggcc tgacggaggg gacgctctat cccctgctct cccgcctccg tctccagggc
    10381 cttgtgacca cgcggctcga ggaatcccct gagggccccg cgcgcaaata ctacgcgctg
    10441 acggcccggg gccggaagat cgctaagcaa atgaacgaac acgtggagca gatgctccaa
    10501 ggaagccggg gcctgcgagc ccggaaggag tgagccatgc tggagtggac ggtatcggcg
    10561 gaagcggaac tggagcgcta cctcgcggag gcgcggacgg cggcgtccgt cagcggggcg
    10621 gacccggagg aggtcgaggg cgacctccgt cgacacatcg agcagcacct ggcggacgcg
    10681 ggcgtccgcc gcgcgacccg ggacgacctg gcccgagtgc tctcccggct taggccggcc
    10741 cggccgacgc ccgcgagcga gcccccggag gcgatcgcca agccgcggcg catctcgctc
    10801 ggtctgatca tggccttcgg cgtcgtgctc cccgtcatca ccctggtgtt tgaagcgttc
    10861 accggcctct gtggcgaact cttcgtcaat cccatcccga cgctctggca cgtcctgctc
    10921 gtcgccaccg taccgctggc caacgccctg gcctggtcgc ggcttgcctt cggacggccc
    10981 gcgagcctga aactcgtcgg cctgttcaac ggattcgcca tcggcgtggc gctctactac
    11041 acgttcgtgt tcctgccggt cacgccgttc gccgcgatcg gcatcatcta cttcgggatc
    11101 ggcctgctgc cgctggcccc gatgctgtcg ctgatcatgg cgctggccgc gcgccgcatc
    11161 ctgcggcgct acgaccggcc ggtcggcgcc ctgccgcccg ccgggcggtg ggcgctggcc
    11221 gcgctgggaa cgatcgccct gctcgccacg cccacgatcg tgacccaggc cggcctgcag
    11281 tgggccacca ccgaatcgcc ggccacgcgc gcgcgtggag tcggactgct ccgcgccctc
    11341 gggaacgatg atctcctgct ccggagctgc tatcgacgcg accgggcggt cggcaacatg
    11401 gtgtcgttcc tcttcgaagg cctcgggcgg cgcatcactt cggagcaggc acagcaggtc
    11461 tactaccgcg tcaccggcac gccgtacaat gccgtgcgcc cgccggaact gcgcgggctg
    11521 cgcacccggg cgctgatcaa cgcggaggat ttcgactggg accagggcgg cgacgcggtc
    11581 gccggccgac tgcgggggtt gagcctccgc gaatcgcggc tcgacagccg gatcgaggcc
    11641 gacagcggcg ccgcgtacac cgagtggatc ctcgtgttcc gcaacgacgc ggagcgccag
    11701 cgcgaggcgc gggcgcaggt cgccctgccg cccggcgcgg tggtctcgcg ggtgacgctc
    11761 tgggtggacg gcgaggagcg cgaggcggcc tacgccgggc gggcgaaggt gaaggccgcc
    11821 taccagcggg tcgtccagca gcggcgcgac ccggtgctcg tcaccaccag cgggcccgac
    11881 caggtcctgg tccaatgctt cccggttccc gcgaatggcg gaaccatgaa gatcaagctc
    11941 ggcatcacgg cgccgctcgc gccggaatcg cgggaggccg gactgcttcg tctcccctat
    12001 ttccgggaga ggaacttctc ggtgcccagg gaagtgaagc attcggcctg gatcgaatcc
    12061 ccgcaaacgg tcgcggcttt gcagccggaa gaccaggtcc tcgccgagca tccttcggag
    12121 cgggtgtacg cgctgcgcgc ggagttggaa gaccgggcgc tttcccggcc ctttgccgtg
    12181 cgggtggcca tggccggcca tcccgcctgg gccgcgctgc gggaagacga aagccgtgtc
    12241 gtccgccagg tcctcgatga gaccccggtg gacaagcccg gccggatcgc tttcgtgttc
    12301 gacggttccc gatccatgag ctccgaagcc gccgccttcg gcgaggtctt gaagtatctt
    12361 cccgccggcg tcgagacggc cgtgttgctg gccggggatc gtccgctgac cgtccgcgaa
    12421 acagccccct gggcgcccga ggcggcgacc gatgtggtga aggcgctctc ggaagcccga
    12481 tatcgcggcg gctgtgacaa tgtgtccgcc ctcgcggagg cctgggacct ggccgcggcc
    12541 gttcccggcg gcacggtggt atggattcac ggcccgcagc ctgtttcgtt cgagggcgag
    12601 gaggcactgc tgcagcgcat cgagcgccgg ccgaacgggc cggtcatcct cgacctgcag
    12661 gtcggcgccg ggccgaacgc ggtggccgtc atgctcaacg gcagtcccgc gctgcgcgcg
    12721 gtggcggatt tcggcgatcc ggcggcggcg ctggcccgca tcctggatgc ctgggccggc
    12781 cgcaaggcgt tctaccgatt tgagcggacg cgtgttccgc gcagcgacat tgccggggat
    12841 attcccgagg gttcctccca tctcgcccgt ctctgggcgt tctccgagat tcttcggctc
    12901 ggcgcctcga tccacgaggc cgacaaggaa gaagccgtgg cgttggccgt gcgctaccgc
    12961 ctggtcacgc cggtgtccgg cgccgtcgtg ctcgagaccg cgaagcagta ccaggaaagc
    13021 gggctggaac ccgcggccgg cgacacggtc ccgcgggtcg tgccggagcc gcagacctgg
    13081 atgctgctcc tgatgggcgg cggtctggct gccgcgcggg cgtggaagcg gaggcgggcg
    13141 cgtccttcat gagcagcccg gtcaggccgc gacgctggcc gggctgaact tcgaccgctc
    13201 ccagaggtgc gcgagcgcgt ccagggcggt cttcagaagc accagcaggg ccaggccggc
    13261 cgcgggtgaa ccgaactgct ggaggagaaa agcgccgccc aggatagcca ggtggaggac
    13321 cacgacgcgg ccgtagggtt gaaacatcag ccgcccgacg tcggcgcgcc ggcactcccc
    13381 tcccgccagg tagttggtca cgaaggaaaa caggtggctg gcggccagcg ccagcagcgc
    13441 ccatcccagc gcggcgcttt ccgccgtctg ccgcacgccc tcctccaccg gctctgctgc
    13501 gccgacactg acctgccccc gttggcggta ccaagttcta tgttagtttg cccccttgtg
    13561 gtcctctccc aaaaactgga caggctagac ctttaggatt tctcccttca acg
//
LOCUS       DAOOTK010000091        13320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3105_length_13320_cov_5.635959, whole genome shotgun sequence.
ACCESSION   DAOOTK010000091 DAOOTK010000000
VERSION     DAOOTK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_13320_cov_5.635959"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PK634_10215"
     CDS             complement(<1..1634)
                     /locus_tag="PK634_10215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOW98149.1"
                     /translation="MNPLSALIWAGFVAGAVSGVSTTVTPPPPDYRLQTGRFGLAAAP
                     VGDFVFAAGGWADDGICDDIEGLDPRKDLALYMAPSISPRFFYSAAAYDGRMILVGGL
                     TEERAITGEVEEFTPEGQGEVGPLPPLPLPVARAGVAVSGDRMYVIGGETPERSRTGK
                     AQIYDFKTKAWSEGAELPVAREGVVVEKAGKIYAPGGYDGGKAIAAFQVYDVAADRWE
                     RLKDLPVKMSAHSAVIADDTLYTFGDYEVMNRTAACDLKTGEWALIDVGYRSSRHNAA
                     VLWSNEVLVIGGNVSSGGPYLDYIQRFPVEKLAAAPRRPWTAENEPKAEPPPAVAAEE
                     EDDEEEAAPAPVPSSKGPAPSAFELSGKPAPDFELELLDGSTFRLADQKDKVVVLDFW
                     ATWCPPCRKTLPHMGELAAAYAGQAVVFLGVSRDRPGDKDKIAPLLAEHKVPYPTGLD
                     LQGLGLDYSVAAIPCVVLIGRDGRVQGRQVGAWDEGPAELRKAIDMLLAGKELPSGKA
                     PPRAAQKAAPPARTARPRPLEESSELDPAFFTLKWRR"
     gene            1773..2717
                     /locus_tag="PK634_10220"
     CDS             1773..2717
                     /locus_tag="PK634_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOW98150.1"
                     /translation="MASRKSASLKNRVKRVVVAGVQIAPRPNDILYNLEKVIAWFRRA
                     VRETGAKLVAFPESITTGFHPNLPLDAFHVLLPPRIDTILAPIRKVCREERAYCVLPT
                     YERGRKRHTIYNSAFLIDARGDVAGVYRKTHPFPAERLGAGGWTTPGVNYPVIPTEFG
                     AVGMMICYDGDFPEVSRILAIKGAQIIARPSALLRAFDIWEMTNKMRAYDNHVYMLAV
                     NAVGSDASGTHYFGHSMIVSPIAQTLALARGTEEIIYSELDPDPLKRLGIGSDTPMHF
                     DHLEDRNVPSYGNLLSRTARSQFEPARRYSVHKPRRKP"
     gene            2714..4342
                     /locus_tag="PK634_10225"
     CDS             2714..4342
                     /locus_tag="PK634_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HOW98151.1"
                     /translation="MNFDRRKKEYAERIRRGRRFARGKRVPKEMADALLGAVLKRGDR
                     VCIEGDNQKQADFLARALLQLDPRRVRDLHMIQSTVALPEHIELFRKGLASRLDFAYS
                     GPQSKALYQLAAEKKVKIGAIHTYLELYGRYVVDLCPQVALLVAEACDEEGNLFTGFN
                     TEDTALIAEATHFKTGVVIVQVKKQLQRLPRVDIPAGWVDFIIVTGEDYHLQPLFTRD
                     PAKITPQQILMAMMSLKGIYAEYGVRALNHGLGYATAAIELLLPTYGKELGLKGKCCT
                     HWVLNPHPTLIPAIEKGFVEHVFSFGGEPGMEKYVQARSDVFSVGPDGHLRSNRCFAH
                     IAGLYAIDLFIGATLQIDRDGNSSTAIKGMIAGFGGAPNLGGSPPGRRNYPPAVAKAG
                     RWESGLFQGRKLVVQMTPTVSEKKQIPVFVEQLDALELHRQGVFAFPPVMIAGEQVTH
                     IVTERGIAYLDKTPDLESRRLAVAAVAGDTPVGRTIHADQADRLRRAGVVKTPEDLGL
                     SPEQATPDRLSARSLKDLVKISKGLYQVPPGCTG"
     gene            complement(4375..5628)
                     /gene="lysA"
                     /locus_tag="PK634_10230"
     CDS             complement(4375..5628)
                     /gene="lysA"
                     /locus_tag="PK634_10230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOW98152.1"
                     /translation="MNAFPMVRGRLMAEGVPVEALARRYGTPLYVYSQSHLASRFAAL
                     AEAMAPVDPLICYSVKVNTNAAVIGTFAGLGAGADVVSGGELYRARRAGVPPSRIVFA
                     GVGKTREEIEYALRANILFFTVESEPELDRIAECARRLGRKARIALRANPDVDPETHH
                     YISTGKAENKFGLDLRRIERAYRRAAAHPSLEIAGLHMHIGSQILNPAPYGRALERVA
                     GLCRRLRARYPTFRYLDLGGGLGIQYRPDQHPLDPARFAKIVLPFMQASGLRIVLEPG
                     RFLVGNAGILVTRVQYVKSGPSKKFIIVDAGMNDLIRPPLYQAHHEIVSVRATRGAVF
                     GDVVGPICESADFLAQDRRLPAVREGDLLAVMSAGAYGFAMASNYNSRGRPAEVLVQG
                     RRHALVRRRERREDLVRGEVAPRWS"
     gene            complement(5688..6908)
                     /locus_tag="PK634_10235"
     CDS             complement(5688..6908)
                     /locus_tag="PK634_10235"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOW98153.1"
                     /translation="MKIVLAYSGGLDTSVILKWLSETYRAEIVAFAADIGQEEELKGL
                     RQKALKTGASKIYIDDLQAEFARDFIFPMFRAGAMYENQYFLGTSIARPLIAKRMVEI
                     AKKEKAQAIAHGATGKGNDQVRFELTAAALAPDLQVIAPWREDRFRDRFPGRTEMIRY
                     CEEHAIPVQATAKKPYSTDRNLLHISYEAGILEDPWLDAFAPANKGMFRLSVSPEDAP
                     DKPELVTLDFVRGDCVAVNGKKLDPLGVMRTLNRLGGKHGVGRVDLVENRFVGMKSRG
                     VYETPGGAILHFAHRQVETLTMDREVMHLRDSLIPKYSELVYYGFWFAPERLALQALV
                     EESQKNVTGTVRVRLYKGGLYVAGRKSPVSLYNPQIATMDADPTRAYHQGDATGFIRL
                     NALRLKVAARRARR"
     gene            complement(7015..8211)
                     /locus_tag="PK634_10240"
     CDS             complement(7015..8211)
                     /locus_tag="PK634_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOW98154.1"
                     /translation="MNTQEIAELHKQYVMPTYAPGLALVRGKGTRVWDAEGNEYLDFL
                     GGIAVNVLGHAHPKLVKAIRQQAARLMHVSNLYFNENQPRLAKALSERSLGGKCFFCN
                     SGAEANEGLFKLARLWGHAQGRHEIVAMTNSFHGRTLATVAATGQTKYQKGFEPMPAG
                     FVHAEYNNLDAVRAAVTDKTVAILVEAVQGEGGVVPAKPEFLAGLRALCDERNLLLLC
                     DEVQCGVGRTGKWFGFQAYGIQPDAFSVAKGLAGGFPIGAIVAGPKLSDVFQPGNHAS
                     TFGGNPLACAAALSVIEAIEEEKLLDHAAAMGELFMAQLRKIAKKFKFIMEVRGLGLM
                     IGMVLDRPAKPLEVLLREKGLIAIATHDTVIRFLPPLNITAGQVRKAARIVKAACAEW
                     TARGLA"
     gene            complement(8323..9195)
                     /gene="argB"
                     /locus_tag="PK634_10245"
     CDS             complement(8323..9195)
                     /gene="argB"
                     /locus_tag="PK634_10245"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOW98155.1"
                     /translation="MQKVIEKADVLIEALPYIQRFRGDTVVVKFGGSAMEEQAQVETV
                     LKDIAFMECVGMRPVVVHGGGKAISQRMKDVGLRPVFVKGLRVTDAASIEVVEQVLNN
                     EVNPSIVRTLEEFGCKARGIHGEHILSVIKHAGQDKDTGEALDWGFVGDVAGVDIEPV
                     KAYLHADIVPVITPLGRGPDGKLYNINADEAAAAIAEKMAARKLVFLSDVPGVLMNPE
                     DPESIVSTLKLTEVEDLIKRGVISGGMLPKIGGALKALKAGVRKTHIIDAALPHSLLL
                     ELFTDKGVGTEILQ"
     gene            complement(9209..10414)
                     /gene="argJ"
                     /locus_tag="PK634_10250"
     CDS             complement(9209..10414)
                     /gene="argJ"
                     /locus_tag="PK634_10250"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOW98156.1"
                     /translation="MTGTKKIAWADEIALPRGFRASGVAAGLKKDSRKDMALIVSDRP
                     AAVAATFTTNQVQAATVKLCRARLACRTGRGVIVNSGNANACTGAQGLKDAERMAQLT
                     GELAGIPPEQVFVSSTGRIGVQLPMDKIEAGIRAAVPALSAEGGTDASLAIMTTDTRP
                     KRVTARLDVDGKPVILSALAKGSGMIEPNMATMLAYILTDAAVEAGALQSCLSAAVEQ
                     SFNRITVDGDRSTNDTVLFFANGAAGNRPLDAAHPDWKTFEHAVRELTLKLALKMVED
                     GEGVSKVVTVRVLGAKTAQDAEWAARSVANSLLVKTSWAGEYPNWGRVMDALGYSRAR
                     VEEDKVDITYDQLPACRGGLYAGTTIDDLKRIVRQKAFTLTIHLNLGAGETVVYTCNC
                     TEEYVRINM"
     gene            10591..11763
                     /locus_tag="PK634_10255"
     CDS             10591..11763
                     /locus_tag="PK634_10255"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOW98157.1"
                     /translation="MNVGIDLISFYTPQYFLGLNVLAQERGVDPDKFTIGIGQEKMAI
                     PPPDEDIVTMAASAAHPILEKVGRENIELLLFATESGIDQSKAAAMFLHGLLKLPARC
                     RAVELKQACYGATAGLHLAAAAVAARPGIKALIVGSDIARYDLRSPGEPTQGAGAVAM
                     LVTVNPRVLALDPETGLYAEDVMDFWRPNYRDEALVDGKYSMRIYLAALEECWKQYAA
                     LTGRKFHDIARYCYHLPFTRMAEKAHIRLAKFAALKELTGEELETEIGDSLRYNRITG
                     NTYSASLYEGLTSLLDHAAEDLSGQRIGLFSYGSGCMGEYFSGVAQPDYRQHLFTTAH
                     RAMLDGRTALTYRQYEDIFQLGFPTDGGDHAFAQYRTGPYRLAGVSQHKRLYERIA"
     gene            11760..12773
                     /locus_tag="PK634_10260"
     CDS             11760..12773
                     /locus_tag="PK634_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOW98158.1"
                     /translation="MKAIAPGKLILSGEHAVVYGRPAIAMAVDRCAQAVVVPSAEAGD
                     TVAIDLQDLQQMESFTVRALRDLKGRMAKNYRMFLDGQLGIRDVLHKPIELFQYAFIT
                     ILDALHLKVAGGMKIQLHSNIPIGCGMGSSAATVLSVLRSIGHYFRVEFRPEWYQAYS
                     LEAENLQHGHASGVDTYISLNGGCARFQEGQAEALPLPRMPIHLVNTGTPATTTGECV
                     AQVRARHANSRIWDDFEAVTRSMEKAVVTNNLKDIQSCVRENQRLLDAIGVVPSRVGE
                     FVADVEKLGAAAKVCGAGSVAGDNAGIVMVCAKEPPKALCAKYSYQLMSIRGEPLGAR
                     IVG"
     gene            complement(12774..13007)
                     /locus_tag="PK634_10265"
     CDS             complement(12774..13007)
                     /locus_tag="PK634_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOW98159.1"
                     /translation="MNVTVEYTGVLDIKTVPSGGVLELPPGATVSALLERLGIDPAHR
                     KYITPLVNNAKAKNSTTLVEGDRIYLLLPVGGG"
     gene            complement(13013..>13320)
                     /locus_tag="PK634_10270"
     CDS             complement(13013..>13320)
                     /locus_tag="PK634_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOW98160.1"
                     /translation="SGGKPGPHKIQGIGAGFVPKNLDTSLLAGVVTVTNDEAFEWGRR
                     LAREEGIFAGISSGANVCAVAKAVKQHGLAGKRVVTIACSFGERYLSTALYKDLAAG"
BASE COUNT         2150 a         4662 c         4371 g         2137 t
ORIGIN      
        1 cgccgccact tgagcgtgaa gaaggccggg tccagttccg acgactcctc gagtggcctg
       61 ggccgcgccg tccgggcagg gggggccgct ttctgcgccg cccggggcgg ggctttgccg
      121 ctcgggagtt ccttgccggc cagcagcata tcgatggcct tccgcaactc cgccgggccc
      181 tcgtcccagg ccccgacctg gcgcccctgc acccgaccgt cgcggccgat gagcaccacg
      241 cacgggatgg cggccacgga atagtcgaga ccgagcccct gcaggtccag gccggtcgga
      301 tacgggacct tgtgctcggc cagcaggggc gcgatcttgt ccttgtcacc cggccggtcc
      361 cggctgacgc cgaggaagac gaccgcctga cccgcgtacg cggcggccag ttcgcccatg
      421 tgcggcagcg tcttccggca cggcgggcac cacgttgccc agaaatcgag cacgaccacc
      481 ttgtccttct ggtcggccag gcggaaagtc gaaccgtcga gcagttccag ttcgaaatcg
      541 ggcgcgggct tgccggagag ctcaaaggcc gacggcgccg ggcccttcga agacgggacc
      601 ggggcgggcg cggcctcttc ctcatcgtcc tcttcctcgg cggcgacggc cggcggcggc
      661 tcggccttcg gctcgttctc cgccgtccac gggcggcgcg gcgcggcggc cagcttctcg
      721 accgggaacc gctggatgta gtccaggtac ggcccgccgg acgagacgtt gccgccgatg
      781 accagcacct cgttgctcca caggacggcg gcgttgtgcc ggctcgagcg gtagccgacg
      841 tcgatcaggg cccactcgcc ggtcttgagg tcgcaggcgg cggtccggtt catcacctcg
      901 tagtcgccga acgtgtagag cgtatcgtcc gcgatcaccg cgctgtgcgc gctcatcttg
      961 acgggcagat ccttcaaacg ctcccagcgg tcggcggcga cgtcatagac ctggaaagcg
     1021 gcgatggcct tgccgccgtc gtacccgccg ggcgcgtaga tcttcccggc cttctccacc
     1081 acgacgccct cgcgggcgac gggcaactcc gcgccctcgg accaggcctt cgtcttgaaa
     1141 tcgtagatct gcgccttccc ggtccggctg cgctcgggcg tctcgccgcc gatgacgtac
     1201 atccggtcgc cggacaccgc gacgcccgcg cgcgccaccg gcaggggcaa gggcggcagt
     1261 ggaccgacct caccctggcc ctccggagtg aattcctcga cctcgcccgt gatggcgcgc
     1321 tcctcggtca ggccgcccac gaggatcatc cggccgtcgt acgcggcggc gctgtagaag
     1381 aagcgcgggg agatggaggg cgccatgtac agcgcgagat ccttgcgcgg gtcgaggcct
     1441 tcgatgtcgt cgcagatccc gtcgtcggcc cagccgccgg ccgcgaacac gaaatcgccg
     1501 accggggccg ccgccaggcc gaaacgcccg gtttggaggc gatagtccgg cgggggcggg
     1561 gtgaccgtgg tgctcacgcc cgagaccgcc ccggcgacga acccggccca gatcagcgcg
     1621 cttaatggat tcatggtcga ttctccgtgt ccgcaggtgt agcgcaggcc ccggcggcgg
     1681 gaaagcaaaa aaccgcccgc acatttgtgc tgacaaatcc ggccgtttgg gaacaattcc
     1741 cgcgttttgc cgaatcggaa aggatggcag ccatggccag caggaagagc gcgtcgctga
     1801 aaaaccgggt caagcgggtc gtcgtcgcgg gcgtgcagat cgcgccccgc ccgaacgaca
     1861 tcctgtacaa cctcgagaag gtgatcgcct ggttccgccg ggcggtccgg gagaccggcg
     1921 cgaaactcgt cgcattcccg gagagcatca ccacggggtt ccatcccaac ctgccgctgg
     1981 acgcgttcca cgtcctgctg cctccgcgga tcgacaccat cctggcgccg atccggaagg
     2041 tctgccgcga ggagcgtgcc tactgcgtcc tgcccaccta cgagcgcggg cgcaagcgcc
     2101 acaccatcta caacagcgcc ttcctgatcg acgcccgggg cgacgtcgcc ggcgtgtacc
     2161 gcaagaccca tcccttcccg gccgagcgcc tcggcgccgg cggctggacg acgcccggcg
     2221 tgaactatcc cgtcatcccg acggagttcg gcgcggtcgg catgatgatc tgctacgacg
     2281 gcgacttccc cgaggtctcg cgcatcctcg ccatcaaggg cgcgcagatc atcgcccggc
     2341 cctcggccct gctgcgcgcc ttcgacatct gggagatgac caacaagatg cgggcgtacg
     2401 acaaccacgt ttacatgctg gccgtcaacg ccgtgggctc cgacgcctcg ggcacgcact
     2461 acttcgggca cagcatgatc gtctcgccca tcgcccagac cctcgccctg gcccgcggca
     2521 cggaggagat catctactcc gagctcgacc cggacccgct caagcggctc gggatcgggt
     2581 ccgacacgcc catgcacttc gaccacctcg aggaccgcaa cgtgccgtcc tatgggaatc
     2641 ttctctcgcg gacggcccgc agccagttcg agccggcgcg ccgctacagc gtccacaagc
     2701 cccggaggaa gccatgaact tcgatcgccg gaaaaaggaa tacgcggagc ggatccgccg
     2761 gggccgccgg ttcgcgcgcg ggaaaagggt gccgaaagag atggccgacg cgctgctcgg
     2821 cgccgtgctg aagcgcggag accgggtctg catcgaaggc gacaaccaga aacaggccga
     2881 cttcctggcg cgcgcgctgc tccagctcga cccgcgccgc gtccgcgacc tgcacatgat
     2941 tcagtccacc gtcgccctgc cggagcacat cgagctgttc cgcaagggcc tcgcctcgcg
     3001 gctggacttc gcctactccg ggccgcagtc gaaggcgctc taccaactcg ccgccgagaa
     3061 aaaggtgaag atcggcgcga tccacaccta ccttgagctc tacgggcgct atgtcgtgga
     3121 cctctgcccc caggtcgccc tgctcgtcgc cgaggcctgc gacgaggaag gcaacctgtt
     3181 caccgggttc aacaccgagg acaccgcgct catcgccgag gccacccatt tcaagaccgg
     3241 cgtcgtcatc gtgcaggtga agaagcagct ccagcgcctg ccgcgcgtgg acatccccgc
     3301 gggctgggtg gacttcatca tcgtcacggg cgaggactac cacctgcagc cgctcttcac
     3361 ccgcgacccg gcgaagatca cgccccagca gatcctgatg gccatgatgt ccctcaaagg
     3421 catctacgcg gaatacggcg tgcgcgcgct gaaccacggg ctcggctacg cgaccgcggc
     3481 gatcgagctg ctgctgccga cgtacgggaa ggaactcggc ctgaagggca agtgctgcac
     3541 gcactgggtg ctcaaccccc accccacgct gatccccgcg atcgagaagg gcttcgtcga
     3601 gcacgtcttc tccttcggcg gcgagccggg catggaaaag tacgtgcagg cgcgcagcga
     3661 cgtcttttcc gtcggcccgg acggccacct gcgctcgaac cgctgcttcg cgcacatcgc
     3721 cgggctctac gcgattgatc ttttcattgg cgcgacgctg cagatcgacc gcgacggcaa
     3781 cagctcgacc gcgatcaagg ggatgatcgc gggcttcggc ggcgcgccga acctgggcgg
     3841 gtcgccgccg ggccgccgga attatccgcc ggccgtggcg aaggccggcc gctgggagag
     3901 cggcctgttc caagggcgca aactggtcgt gcagatgacg ccgacggtca gcgagaagaa
     3961 acagatcccc gtgttcgtcg agcagctcga cgcgctggag ctgcaccggc agggcgtctt
     4021 tgcgttcccg ccggtgatga tcgcgggcga acaggtcacg cacatcgtca ccgagcgcgg
     4081 gatcgcctat ctcgacaaga ccccggacct cgaatcgcgc cggctcgccg tcgccgccgt
     4141 agcgggcgac acgcccgtcg gaaggacgat ccatgcggac caggccgacc gccttcgccg
     4201 cgcgggcgtg gtgaaaacgc cggaagacct gggcctgtcg ccggaacagg ccacgcccga
     4261 ccggctgtcc gcgcggagcc tcaaggacct ggtgaagatt tcaaaaggcc tctaccaggt
     4321 gccgccgggc tgcacgggtt aacggggaca ggcgcgggtg gaacccggcc ttgttcacga
     4381 ccagcgcggg gcgacctccc cgcgcaccag gtcctcccgg cgttcccggc ggcggacaag
     4441 ggcgtggcgg cggccctgca ccagcacctc ggccgggcgg ccgcggctgt tgtagttcga
     4501 agccatggcg aacccgtagg cccccgcgct catgacggcc agcaggtcgc cctcgcggac
     4561 ggcgggtagc cgccggtcct gcgccaggaa atccgccgac tcgcaaatcg ggccgacgac
     4621 gtcgccgaag accgccccgc gcgtcgcgcg gacggagacg atctcgtggt gggcctggta
     4681 caggggcgga cggatcaggt cgttcatccc ggcgtccacg atgatgaact tcttcgacgg
     4741 cccgctcttc acatactgga cccgcgtcac caggatcccc gcgttaccga cgaggaaccg
     4801 gcccggctcc aggacgatcc gcaggcccga cgcctgcatg aacggcagga cgatcttcgc
     4861 gaaccgcgcc ggatccagcg gatgctggtc gggccggtac tggatgccca gcccgccgcc
     4921 gaggtccagg tagcgaaacg tcgggtagcg ggcgcgcagc cggcggcaga gcccggccac
     4981 ccgctccagc gcgcgcccgt acggcgcggg attcaggatc tgcgaaccga tgtgcatgtg
     5041 caacccggcg atctccagcg acgggtgcgc cgccgcgcgg cggtacgccc gctcgatgcg
     5101 acggaggtcc aggccgaact tgttctcggc cttgcccgtg ctgatgtagt ggtgcgtctc
     5161 cgggtccacg tccgggttcg cgcgcagggc gatgcgggcc ttccggccca ggcgccgggc
     5221 gcactccgcg atccggtcca actccggctc ggactccacg gtgaagaaca ggatgttcgc
     5281 ccgcaaggcg tactcgatct cctcgcgggt cttgccgacg cccgcgaaga cgatgcgcga
     5341 cggcggcacg cccgcccggc gcgcgcggta cagctcgccg ccggagacca cgtccgcccc
     5401 cgcgccgagc cccgcgaacg tcccgatcac ggccgcgttg gtgttgacct tgaccgagta
     5461 gcagatcagc gggtcaaccg gcgccatggc ctccgccagc gccgcgaatc gggatgcgag
     5521 atgagattgg ctgtagacgt aaagcggcgt cccgtagcga cgcgccagcg cctccaccgg
     5581 caccccctcg gccatcaacc gcccgcgaac catgggaaaa gcattcatgc tgaagcgcga
     5641 ctgtacgcga gtgcacgcga cttggccaat tcaaacggca gccgcgccta tctgcgcgct
     5701 ctgcgggccg caaccttcag ccgcagcgcg ttgagccgga tgaaccccgt cgcgtcgccc
     5761 tggtggtagg cgcgggtggg gtcggcgtcc atggtcgcga tctgcgggtt gtagaggctg
     5821 accgggctct tgcgaccggc gacgtacagg ccgcccttgt acaggcgcac ccggaccgtg
     5881 ccggtgacgt tcttctggct ctcctcgacc agcgcctgga gggcgagccg ctcgggcgcg
     5941 aaccagaagc cgtagtagac cagctcggaa tatttcggga tcagcgaatc gcgcaggtgc
     6001 atcacctcgc ggtccatcgt cagcgtctcg acctgccggt gcgcgaaatg caggatcgcg
     6061 ccgccgggcg tctcgtagac gccccggctt ttcatgccga cgaagcggtt ctccacgagg
     6121 tccacccgcc ccaccccgtg cttgccgccc agccggttca gggtccgcat cacacccagc
     6181 gggtcgagct tcttcccgtt caccgcgacg cagtcgccgc gcacaaaatc cagcgtaacg
     6241 agctccggct tgtccggcgc gtcctccggc gagacggaga ggcggaacat gcccttgttc
     6301 gcgggcgcaa aggcgtccag ccacgggtct tccaggatgc cggcctcgta ggagatgtgg
     6361 agcaggttcc ggtcggtcga gtacggtttc ttcgccgtgg cctgcacggg gatggcgtgc
     6421 tcctcgcagt agcggatcat ctccgtgcgg ccggggaacc ggtcgcggaa ccggtcctcg
     6481 cgccacgggg cgatgacctg caggtccggc gccagcgcgg cggcggtcag ctcgaagcgg
     6541 acctggtcgt tgcccttgcc cgtcgcgccg tgggcgatgg cctgggcctt ttccttcttc
     6601 gcgatctcga ccatgcgctt ggcgatcagc ggccgcgcaa tggaggtccc gagaaaatac
     6661 tggttctcgt acattgcccc ggcgcggaac atggggaaga tgaaatcgcg cgcgaactcc
     6721 gcctgcaggt cgtcgatgta gatcttgctc gcgccggtct tcagcgcctt ctgccgcagg
     6781 cccttgagct cctcctcctg cccgatatcc gccgcgaagg cgacgatctc ggcccggtag
     6841 gtctccgaaa gccacttcag gatgacggac gtgtccagcc cgccggaata tgcgagtacg
     6901 attttcatgg cttgctcctg ctgaatgtcg gttcgccgga ggctcgccct ccagcggaat
     6961 gcgaaaaata taatggaggg cgagcgtccc cgcgagccgt tattgggcca ctcttcaggc
     7021 caatccccgg gccgtccact ccgcgcaggc ggccttgacg atgcgcgcgg ccttccgcac
     7081 ctggcccgcc gtgatgttca gcggcggcag gaaccggatc accgtgtcgt gcgtggcgat
     7141 ggcaatcagg cctttctccc gcagcagcac ttcgagcggc ttggcgggcc ggtcgaggac
     7201 catgccgatc atcaggccga gtccccggac ctccatgatg aacttgaact tcttcgcgat
     7261 tttccggagt tgggccatga acagctcgcc catcgcggcg gcgtgatcga ggagcttctc
     7321 ctcctcgatg gcctcgatga ccgaaagcgc cgcggcgcac gccagcggat tgccgccgaa
     7381 cgtgctggcg tggttgcccg gctggaagac gtccgacagc ttcggcccgg cgacgatcgc
     7441 gccgatcggg aagccgcccg cgaggccctt ggccacggag aaggcgtccg gctggatccc
     7501 gtaggcctgg aacccgaacc acttgcccgt ccggccgacg ccacactgga cctcgtcgca
     7561 gagcagcagc aggttccgct cgtcgcacag agcccgcagc ccggccagga attccggctt
     7621 cgcgggcacg acgccgccct cgccctggac cgcctcgacg aggatggcga cggtcttgtc
     7681 cgtcacggcg gcccggacgg cgtcgaggtt gttgtactcg gcgtggacaa agcccgccgg
     7741 catgggctcg aagcccttct ggtacttcgt ctggcccgtc gcggccacgg tggcgagcgt
     7801 ccggccgtgg aacgagttgg tcatggcgac gatctcgtgc cgcccctgtg cgtggcccca
     7861 gagccgggcg agcttgaaca ggccctcgtt ggcctccgcg cccgagttgc agaagaagca
     7921 cttgccgccg agcgaccgct cggacagcgc cttggcgagg cgaggctggt tctcgttgaa
     7981 ataaagattc gagacgtgca tcagccgggc ggcctgctgc cggatggcct tgaccagctt
     8041 cgggtgggcg tggcccagca cgttgaccgc gatgccgccg aggaagtcga gatattcgtt
     8101 gccctcggcg tcccagaccc gcgtgccctt gccccgcacg agcgcgaggc ccggcgcgta
     8161 ggtcggcatg acgtactgct tgtgaagttc ggcgatttct tgtgtattca tggcggggat
     8221 acggaacaac tttcaccgac taattgcaac cgcgaattaa cgcccattaa cgcgaatggc
     8281 cggagcagac cctcctcctc attagcgtta attggcggtg tctcattgca ggatctcggt
     8341 gccgacgccc ttgtcggtga agagctccag cagcagcgag tgcggcaggg cggcgtcgat
     8401 gatgtgcgtc ttgcgcacgc cggccttgag ggccttgagc gcgccgccga tcttcggcag
     8461 catcccgccg ctgatcacgc cgcgcttgat gaggtcctcc acctcggtca gcttgagcgt
     8521 ggacacgatg gactccgggt cctccgggtt catcagcacg ccgggcacgt cggagaggaa
     8581 caccagcttg cgcgccgcca tcttctccgc gatcgccgcc gcggcctcgt ccgcgttgat
     8641 gttgtagagc ttgccgtcgg gcccgcgccc gagcggcgtg atcaccggga cgatgtcggc
     8701 gtggaggtag gccttgaccg gctcgatatc cacgccggcg acgtcgccga cgaagcccca
     8761 gtccagcgcc tcgcccgtgt ccttgtcctg gccggcgtgc ttgatgaccg acaggatgtg
     8821 ctcgccgtgg atcccgcggg ccttgcagcc gaactcctcc agcgtgcgga cgatggacgg
     8881 gttgacctcg ttgttcaaca cctgctccac gacctcgatc gaggccgcgt cggtgacccg
     8941 caggcccttg acgaacacgg gtcgcaggcc gacgtccttc atgcgctggc tgatcgcctt
     9001 gccgccgccg tggacgacca ccggccgcat cccgacgcac tccatgaacg cgatatcctt
     9061 gagcacggtc tccacctgcg cctgctcctc catcgcgctc ccgccgaact tcacgaccac
     9121 cgtgtcgccg cggaatcgct ggatgtacgg cagcgcctcg atcaggacat ccgctttctc
     9181 gatgactttt tgcatggccg ccccgctcct acatgttgat ccgcacgtat tcctcggtgc
     9241 agttgcacgt ataaaccacc gtctcgccgg cgccgaggtt caggtggatc gtcagcgtga
     9301 acgccttctg ccgcacgatc cgcttcaggt cgtcgatcgt cgtgccggcg tacagcccgc
     9361 cccggcaggc cgggagctgg tcgtacgtga tatccacctt gtcctcctcg acccgcgccc
     9421 gggaatagcc cagcgcgtcc atcacccggc cccagtttgg atattcgccc gcccacgacg
     9481 tcttcacgag cagcgagttc gcgaccgacc gcgccgccca ctcggcgtcc tgcgccgtct
     9541 tcgcgccgag caccctgacg gtgaccacct tcgagacgcc ttccccgtcc tcgaccatct
     9601 tcagcgcgag cttcagggtc aactcccgca cggcatgctc gaaggtcttc cagtccgggt
     9661 gggcggcgtc cagcggccgg ttgcccgccg cgccgttggc gaagaagagc accgtgtcgt
     9721 tcgtgctccg gtcgccgtcg acggtgatcc ggttgaagct ctgctcgacg gccgcggaca
     9781 ggcaggactg gagcgccccg gcctcgaccg ccgcgtcggt caggatgtac gccagcatcg
     9841 tggccatgtt cggctcgatc atccccgagc ccttggccag ggcggaaaga atcacgggct
     9901 tgccgtccac gtccaaccgc gcggtgaccc gcttcggccg cgtgtcggtg gtcatgatcg
     9961 cgagcgaggc gtcggtcccg ccctcggcgg agagcgcggg cacggcggcc cggatgcccg
    10021 cctcgatctt gtccatcggc agctgcacgc cgatccggcc ggtggagctg acgaagacct
    10081 gctccggcgg gatccccgcc aattcgccgg tcagttgcgc catccgctcc gcgtccttca
    10141 ggccctgggc gccggtgcag gcgttggcgt tgccgctgtt gacgatcacg ccgcggccgg
    10201 tccggcacgc gagccgggcc cggcaaagct tgaccgtcgc ggcctggacc tggttggtgg
    10261 tgaacgtcgc ggccacggcc gcgggccggt cggagacgat caacgccatg tccttccggc
    10321 tatccttctt cagccccgcg gccacgcccg aggcccggaa gccccgcggc agcgcaattt
    10381 cgtccgccca tgcgatcttc ttcgtgcccg tcatcgttcg gctccttcgc gaaaaaacgg
    10441 gagtactata tcgcatccgc gccgcgcgtc acgcgaatcg tgtgaacatc cgcgcgggcc
    10501 atgtgtctga agaactatgg gcccggatgt ggccgcctgc atggcagcgc ttgccgacac
    10561 ggtcacatcg gggttagact gcttctcgtc atgaatgtcg gcatcgatct catcagcttc
    10621 tacacgccgc agtacttcct gggcctgaac gtgctggccc aggagcgggg cgtggacccg
    10681 gacaagttca cgatcggcat cggccaggag aagatggcca tcccgccgcc ggacgaggac
    10741 atcgtcacca tggccgccag cgcggcgcac ccgatcctgg aaaaggtggg gcgggagaac
    10801 atcgagctcc tcttgttcgc cacggagagc gggatcgacc aatccaaggc ggcggcgatg
    10861 ttcctgcacg ggctgctcaa gctgccggcg cgctgccggg ccgtcgagct gaagcaggcc
    10921 tgctacggcg ccacggcggg cctgcacctg gcggccgcgg ccgtcgcggc acggccgggg
    10981 atcaaggcgc tgatcgtcgg ctcggacatc gcgcgctacg atctgcgcag ccccggcgag
    11041 ccgacgcagg gcgccggcgc agtcgccatg ctggtgaccg tcaacccgcg cgtcctggcg
    11101 ctggacccgg agacggggct gtacgccgag gacgtgatgg atttctggcg gcccaactac
    11161 cgcgacgagg cgctcgtgga cggcaagtac tccatgcgca tctacctggc cgcgctggag
    11221 gaatgctgga agcagtacgc cgcgctcacc ggccggaagt tccacgacat cgcccgctac
    11281 tgctaccacc tgcctttcac gcgcatggcc gagaaggcgc acatccggct cgccaagttc
    11341 gcggcgctca aggaactgac cggcgaggaa ctggagaccg agatcggcga ttcgctgcgg
    11401 tacaaccgga tcacgggcaa tacgtattcg gcctcgctgt acgaggggct gacctcgctg
    11461 ctcgaccacg ccgccgagga cctgtccggc cagcggatcg gcctgttcag ctacgggtcg
    11521 ggctgcatgg gcgagtattt tagcggcgtc gcgcagccgg actatcgcca acaccttttc
    11581 acgacggcac atcgggcgat gctggacgga aggacggcat tgacgtatcg gcaatacgag
    11641 gatatcttcc aactgggctt cccgacggac ggcggtgacc atgcgttcgc ccaataccgc
    11701 acggggccgt accggcttgc gggggtcagc cagcataaac ggttgtacga aaggatagca
    11761 tgaaggccat cgcgcctggg aaactgatct tgagcggcga gcatgccgtg gtgtatggcc
    11821 gaccggccat cgccatggcc gtggaccgct gcgcccaggc cgtcgtggtc ccgtccgcgg
    11881 aagccggcga cacggtcgcg atcgatctcc aggatctgca gcagatggaa tccttcaccg
    11941 tgcgcgccct gcgcgacctg aaaggccgga tggcgaagaa ctaccggatg ttcctggacg
    12001 gccagctcgg catccgcgac gtgctgcaca agcccatcga gcttttccag tacgccttca
    12061 tcaccatcct cgacgcgctg cacctgaagg tggccggcgg gatgaagatt cagttgcact
    12121 ccaacatccc gatcggctgc ggcatgggct cgtcggcggc gacggtgttg agcgtgctgc
    12181 ggagcatcgg ccactacttc cgcgtcgagt tccgccccga gtggtaccag gcctacagcc
    12241 tcgaagccga gaacctccag cacggccatg ccagcggcgt ggacacgtat atctcactga
    12301 acggcgggtg cgcgcggttc caggaaggcc aggccgaggc cctgcccctg ccgcgaatgc
    12361 cgatccacct ggtcaatacc gggacgccgg ccaccaccac gggcgaatgc gtggcccagg
    12421 tgcgggcgcg gcacgcgaac agccggatct gggatgactt cgaggcggtc acgcgcagca
    12481 tggaaaaggc cgtcgtcacg aacaacctca aggacatcca gtcctgcgtc cgcgagaacc
    12541 agcggctgct cgacgccatt ggggtcgtgc cctctcgcgt aggcgagttc gtggcggacg
    12601 tcgagaaact aggcgccgcg gccaaggtct gcggcgcggg ctcggtggcc ggcgacaacg
    12661 cgggcatcgt catggtctgc gcgaaggaac cgcccaaggc gctgtgcgcg aaatacagct
    12721 accagctcat gagcatccgc ggcgaaccgc tcggcgcgcg aatcgtcggg taatcagcct
    12781 ccgccgacgg gcagcagcag gtagatccgg tccccctcca ccaaggtcgt ggaattcttt
    12841 gctttggcgt tgtttacgag aggcgtgatg tacttgcggt gcgccggatc gatccccaac
    12901 cgctccagca gggccgacac cgtggcgccc ggcggcagct ccagcacgcc gccgcttggg
    12961 acggtcttga tatccagcac cccggtatat tccacggtaa cattcatgag gatcagcccg
    13021 ccgccaggtc cttgtacaac gccgtggaaa gatagcgctc cccgaagctg caggcaatcg
    13081 taacgacacg ctttccggcc aggccgtgct gtttgaccgc cttggccacg gcgcacacgt
    13141 tggccccgct gctgatgccc gcgaagatcc cttcctcgcg cgcaagacgg cggccccact
    13201 cgaaggcctc gtcgttggtc accgtcacca cgccggccag gagcgaggtg tccaggttct
    13261 tcggcacgaa gcccgcgccg atgccctgga tcttgtgcgg gccaggcttg ccgccgctga
//
LOCUS       DAOOTK010000092        13194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3145_length_13194_cov_5.676992, whole genome shotgun sequence.
ACCESSION   DAOOTK010000092 DAOOTK010000000
VERSION     DAOOTK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3145_length_13194_cov_5.676992"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PK634_10275"
     CDS             complement(<1..1304)
                     /locus_tag="PK634_10275"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOW98161.1"
                     /translation="MLKTKLFQAFAALAIVFGLLSSVIGIQIIRKRVMGEAQNQVSLD
                     IGSAWAIFNSKLHDLETVLDLVAIKKIVVDAASEHDWASEDIQQRLETIRTSFGLDFL
                     TVVSPEGQVVLRAAPPYRTGDFQKQNPVVLRALKGEHASGVTLMSQPELDLEAEGLSE
                     KAFLVLEKTPRARATPLTEESRGMVLATGVPIFKGPQLLGAVYAGVLLNRNQDLVDRM
                     TEVIYKNEQYKGLAMGTATIFLKDCRVATTVRLPNGNRAIGTRVSKEVADRVLDNGLP
                     WIGPAFVVKEPYLTAYDPIRGFDGEVIGMLYVGRLERPFKALSRNIMMRYAGLSVFGL
                     AAALVLAFIMAGRLANPIHRLVEASQRMRKGEPHTPVECRSSCGEIENLVVAFNEMAT
                     ALEEREGRLKEANEKLEKTNEAVSALNLSYMDMLGFVSHELK"
     gene            complement(1317..1943)
                     /locus_tag="PK634_10280"
     CDS             complement(1317..1943)
                     /locus_tag="PK634_10280"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOW98162.1"
                     /translation="MLSSENTGVPARTVERLVLYRRLLNGLTEQGVEFIHSHEIAEKA
                     NNSAAQVRRDLMAIGYTGNPARGYAVNDLVRSINGLFEQKRDQKAALVGIGKLGRAIL
                     GYFALRQARPRIVAAFDSDPAKGQGEFAGCPCYPVDALTSVIARDGITVGILTVPADF
                     AQPVTDLMLVAGVRGILNFAPTPLKTPAWAVVDNVDIATKLEKVAFFC"
     gene            2164..2814
                     /locus_tag="PK634_10285"
     CDS             2164..2814
                     /locus_tag="PK634_10285"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOW98163.1"
                     /translation="MSRINRPGLLIFAAATLGFAAPARSDSIEWCDSRAAAVEQALNS
                     GRLILLLAGRESCGNCHYMKYTVCETNSVRSILDADYVCWFCPVDTSTEENPYTGGLG
                     SYTLPLICVIDPGNPLAYLDRTTGIQYEPTFVPRLLGHLPPVTAAVERIEWTENGALR
                     VVMSGATGFSYRVLSADSLAGSWRTNGAAAAGPGGVCSNDLPPSAAGGFFKVIGFR"
     gene            complement(2824..4038)
                     /locus_tag="PK634_10290"
     CDS             complement(2824..4038)
                     /locus_tag="PK634_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOW98164.1"
                     /translation="MKKAIAVFGFIMMSWCASAQIVQDGDMEAADTNAWLDWSVPFVK
                     AKEFDAERGSTVLHVATGGVQQLNLPVTAGAEYTLRLSAKVKTGTLRVVLGIGDSNSD
                     FENRYYDLSAANYGTWNAMERRFTVPAGFTHDFRLALVASAGEAWADDVEITAGQDAR
                     IVLDGDMENAQLGQWWRWGTPELKEKSAADPHAGAQSLHFRTFSGTNWIANRSGLQQR
                     FVPVATGRWYRLSFWYRVASGTLCPRLGHNDSNNDYQLGLGEFPVLAATGGEWRFYER
                     AFRTPDAITGDFRIVLPLNPYNYLTRTTNDFAEAEVDDLAIEPYDAKRDLIPDLDIAV
                     TGAEGLFQWPVTNGLVYSLEYTTNLTPGSVWRAVRNYGGLPGFDGQCSATVDVSKVLT
                     GYSNFFFRVRAQ"
     gene            complement(4198..4749)
                     /locus_tag="PK634_10295"
     CDS             complement(4198..4749)
                     /locus_tag="PK634_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HOW98165.1"
                     /translation="MNRRVLLVLFLAACVAQWVAATWKIAERELILRLGHVYKFKTAP
                     MDPVDAFRGRYVAVRVEQNTAPFEGPWTRKQGGWFFAELAVGEDGFARFVRLHRRPPA
                     GAPAIRTRIASFNVQEQKAWLNLPLDRFYMSERQAPEAERAYREHSRQDAQDAWLAVR
                     VWRGRAAIENLYIGDRPILDVVR"
     gene            complement(4746..6035)
                     /locus_tag="PK634_10300"
     CDS             complement(4746..6035)
                     /locus_tag="PK634_10300"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOW98166.1"
                     /translation="MTRKGNIQWLLGELPGWVGQGVLTPEQAESLRRHYRSAQPAAGR
                     GWLLTLFAILGAVLIGFGIILLLAHNWEQISRPVRAGMSLALLLFGQVMAGRAVVRQA
                     AAAGLREASSAFLMLVAGASLALIAQTYHVAADMESFILTWMLLGLPLVYTHRAVLPA
                     VFYLAGVSSWSLMALETCGPTGYWLLIALLLPFLWARIREDRYQARAALLLWGLAVSL
                     LILSGVITGEQEDEYLLLMFSLLLASFYLADALWLEGAPTAWQRPTKLLGAGGIVIMA
                     FILSFEWEAGRLSFQEYAAFVGGGLSGVLLGVCLPLLYLATAGRAVLGGRGGRMVFGV
                     FPLLVWIGVLAGQGRGWLIANGYLFGLGLFLLVSGARQRQLGKTNGGMLILGALIIIR
                     FFDSDLSFVAKGIVFILLGLGFLGANWIMLRRKGGAP"
     gene            complement(6044..6208)
                     /locus_tag="PK634_10305"
     CDS             complement(6044..6208)
                     /locus_tag="PK634_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98167.1"
                     /translation="MNQLVQLGIVLGIVALCVVGLGFALTRRNRRHGSCSCKGLSGEK
                     SSCACSSPES"
     gene            complement(6205..6519)
                     /locus_tag="PK634_10310"
     CDS             complement(6205..6519)
                     /locus_tag="PK634_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOW98168.1"
                     /translation="MSRALPLAGLADYQAGAVVSRTILSKKAGTVTVFAFDKGQGLST
                     HSAPYDAMVWILDGEARITISGKGLRAKTGDMVIMPANQPHALKAAKPFKMALVMIKS
                     ES"
     gene            6643..8544
                     /locus_tag="PK634_10315"
     CDS             6643..8544
                     /locus_tag="PK634_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW98169.1"
                     /translation="MKRLLHSSLLFALLAGHPAAGEWQDKVDPRVLQSVDGSGDAQTE
                     FLVFLQERADLSGADALRTKDEKGRFVYRALTQTAARTQPALRARLQTLGAIQQAFWI
                     DNMLLVHGNASLVRELAERPDVRRLSANPWVDARLPRDRAAPRAPDAPAAAEWNISKI
                     NATGAWALGFTGQGVIVGGQDTGYDWDHAALKAQYRGWDGESADHNYNWHDAIHSGGG
                     GCGPDSPVPCDDGTHGTHTMGTMVGDDGTNQVGVAPGARWIGCRNMNVGWGTPATYAE
                     CFQWFVAPTDLANGNPDPARAPDVINNSWGCPTAEGCTDPNVLSGVVAAARAAGIVVV
                     VSAGNSGSSCGTVSDPPSFYEDSFTVGNTDSSDNINSGSSRGPATADGSGRMKPDISA
                     PGTSVRSCVPGTGFGYKTGTSMAGPHVAGTVALVLSAHPGLRGQVDRIEKLLTRTALP
                     RTTTQVCGGLSGTNVPNNTYGWGRVDAYRAVGLDDDDADGMPNWWEIVFDLNPTNAAD
                     AGFDPDGDGLLNGQEFSANTDPTNGASVLQLIEALPSESNAVAVAWQSGQEGFDSGRT
                     YDLYRSTSMADGDGWTCVASNIPVAGALTGFTNVWDSGTVPQAYYRVAISGASNEVSS
                     APIGVVPRP"
     gene            8687..12058
                     /gene="metH"
                     /locus_tag="PK634_10320"
     CDS             8687..12058
                     /gene="metH"
                     /locus_tag="PK634_10320"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOW98170.1"
                     /translation="MDLLARILQGVFVMDGAMGTQIQARAVPADAWQGRDGCNELLNV
                     TAPDIIGSIHRAYFEAGSDAVETNTFGASPITLGEYELSGRAREINRAAARLAREAAA
                     SFTAPEKPRFVFGSIGPGTRLPTLGQVGFDELRAALRVQIEGLLEGGADGILLETCQD
                     LLQIKAGLAAYDDVVGRSAKVPLYVSVTVEQTGTLLIGSSIAAVVAALSPYPIHILGL
                     NCATGPEAMRLHLDYLAANWPRLLACMPNAGLPEMKAGGVRYPLGPAFFAEKLGAMAR
                     EVGLNVVGGCCGTTPDHIRALAQGLRGFQAPARAARAPQQAASVFSPVDLTQDPPPLY
                     IGERANATGSKKFRDALLADKYDDAFAVLPEQEEASAHVLDLSCAYAGRDEKKDMEIL
                     VARAARECRAPLMIDSTQADVTEAALKLYGGRALVNSINFESGEERARHVAGLARKFG
                     AGLVCLTIDETGMAMTADRKVAIARRLVEFCEAEGIRRDALFVDTLTFTIGSGDPTLR
                     TAALETLEAIRRIKKELPDVRTILGLSNISFGLKPAGRKVLNTVFLDRCLKAGMDACI
                     INVASIVPLTDIAPDALRVAGALLDNDASAGDPLENFIRFFEGAVDAGAEAAAAALPP
                     AEALARAVIKGRPQPLEAAIPALLGQHPAEFILNEILIPAMKEVGRLFNDGILQLPFV
                     LKSAEVMKKAVDLLKPHMKKDESGGRRGLMVLATVAGDVHDIGKNLVDIILSNNGFHV
                     VNLGTKIPIERMIAAVREHKADVLGMSGLLVKSAAIMAENMKALEAAGIRIPVFLGGA
                     ALTPGFVADSCQPRYSAPVVYCKDAFEGLARMRELSETGRLEKAQPRPPSAPPPDFEA
                     PPVSIDLSLPPPKPPFLGHRITESIRLEDVYPHLNETALIRGRWGYRRGKMGAEEYDR
                     LIEDEVKPRLEALKKQCAESGLFRPKAAHGWFWCRAEGEALTVFPSEGGEPIRMVFPR
                     QSRSPGLSIPDFFRRDEDVIGFMVVTLGPGLETENARLLQRDHYQDYFLLHGLAVEVT
                     DALAEYWHARMRAELGFTEPKLAIQDYIVQKYRGSRYGFGYPACPDLSMNKVCCDLVH
                     AADIGVSLSDNFMMVPEVTTSALVAHHPAAKYFNV"
     gene            12103..>13194
                     /locus_tag="PK634_10325"
     CDS             12103..>13194
                     /locus_tag="PK634_10325"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOW98171.1"
                     /translation="MTSGSHTSPPVFWLVSGISLLLYTITLAPSVTLEYSGSLVTAAD
                     SLGVANPPGYPVWTLLAWFFQKLFGFVRYHGAPNPAWGVNFMSAFFGSLSCGLVAHMV
                     SRSVRSESRGVSDPQASCAAWIAGTSAGLLFAFSPFMWAQSVIAETNTLNVFSVVLFL
                     ALLQGGVLDDGKRFLYPLAFLLGIGFLNSPTFVLMIPLFAAAWAKRGTWRQVPALLLC
                     LALGLLPMAYLPLASAQNPPVNWGYARTLDGFIHLVTRGQYEHMTLTPVFSAPDVFLA
                     QMATYIRLLAAQFSTPVLVLGIPALFGMRHGGLWKPALLAAFLLYGTGTTIGMNPPLD
                     HQTLYYCRVVWIPSYALAAILIGLGLGWIF"
BASE COUNT         2231 a         4502 c         4292 g         2169 t
ORIGIN      
        1 ctcttcagtt cgtgcgaaac gaagcccagc atgtccatgt agctcaagtt gagggcggag
       61 acggcctcgt tggtcttctc cagcttctcg ttggcttcct tcaggcggcc ctcgcgctcc
      121 tcgagggccg tggccatctc gttgaaggcg acgacgaggt tctcgatctc gccgcaggaa
      181 ctccggcatt cgacgggggt gtgcggctcg cccttccgca tgcgctgcga ggcttccacc
      241 agccggtgga tgggattcgc cagccggccg gccatgatga aggccaggac cagcgcggcc
      301 gcgaggccga aaaccgacag gccggcgtac cgcatcatga tattccggct cagggccttg
      361 aacggccgct ccagccggcc gacgtacagc atgccgatga cctcgccgtc gaagccgcgg
      421 atgggatcgt aggcagtgag gtacggctcc ttgaccacga aggcggggcc gatccacggc
      481 aggccgttgt ccaggacgcg gtccgcgact tccttggaga cgcgcgtgcc gatcgcccgg
      541 ttgccgttgg gcagacgcac ggtggtggcg acgcggcagt ccttcaggaa aatcgtcgcc
      601 gtgcccatgg cgaggccctt gtactgctcg ttcttgtaga taacctccgt catccggtcc
      661 acgaggtcct ggttgcggtt gagcaaaacg ccggcgtaga ccgcccccag caactggggg
      721 cccttgaaaa tcggcacgcc ggtcgcgagg accatgcccc gactctcctc ggtcagcggg
      781 gtcgcccggg cgcgcggggt cttctccagc accaggaacg ccttctcgga caggccctcg
      841 gcctcgaggt ccagctcggg ctgcgacatc agcgtcacgc cggaggcgtg ctcccccttc
      901 agcgcgcgca gcaccacagg gttctgcttc tggaagtcgc cggtacggta cgggggcgcg
      961 gcgcgcagca ccacctggcc ttccggcgag accacggtca ggaaatccag gccgaagctc
     1021 gtgcggatgg tctcgagccg ctgctggatg tcctcggacg cccagtcgtg ttcggatgcg
     1081 gcgtccacca cgatcttctt gatggcgacg agatccagga ccgtttccag gtcgtgcagc
     1141 ttggaattga agatcgccca tgcgctgccg atgtcgaggc tcacctggtt ctgcgcctcg
     1201 cccatcaccc gcttgcggat gatctggatg ccgatgacgg aggagagcag cccgaacacg
     1261 atcgccagcg ccgcgaacgc ctggaatagt ttggtcttaa gcatggaggc cgtacctcaa
     1321 cagaaaaacg ccaccttctc aagcttcgtc gcaatgtcca cgttgtccac caccgcccat
     1381 gccggcgtct tcagcggcgt cggcgcgaaa ttcaggatgc cccggacgcc ggcgaccagc
     1441 atcaggtccg tcaccggctg ggcgaaatcc gcggggacgg tcaggatgcc gaccgtgatg
     1501 ccatcgcggg cgatcacgga cgtcagcgcg tccaccggat agcacgggca gccggcgaac
     1561 tcgccctgtc ccttggccgg gtcgctatcg aaggccgcga cgatccgcgg ccgggcctgg
     1621 cgcagcgcga agtacccgag gatcgcgcgc ccgagtttgc cgatgccgac gagcgcggcc
     1681 ttctggtcgc gtttctgctc gaaaagcccg ttgatgctgc ggaccaggtc gttcaccgcg
     1741 tagccgcgcg ccgggttgcc ggtgtagccg atcgccatca ggtcgcgccg cacctgggcg
     1801 gccgaattat tggccttctc ggcgatttcg tgggagtgaa tgaactccac gccctgctcg
     1861 gtaaggccat tcaatagccg gcgataaagc acaagccttt ctacggtcct cgccggaact
     1921 cccgtatttt cagaagataa catttatttc gttatccgat tcacattgtg aatcatttca
     1981 caatcagaca atacccggca ttcttctggc ggtcaaccag aaaatcaacc tgcctgttga
     2041 tccgtcttgt acggcccccc gtccctggat gaaatggcca agccgggcgc atggcgatcc
     2101 attcttcatt tccatttgcg gacgcggctt tctggggtaa tcatacgacg gtctctggaa
     2161 accatgtctc gcatcaacag gcccggcctc cttatcttcg cggcggcaac cctcgggttc
     2221 gccgctcccg cgcgaagcga ttccattgaa tggtgcgatt cccgggccgc ggcggtcgag
     2281 caggcgctga attccggacg gctcatcctg ctgctggcgg gacgggagtc ctgcgggaac
     2341 tgccactaca tgaagtacac ggtctgcgag acgaactcgg tgcgctcgat cctcgatgcg
     2401 gactacgtct gctggttctg ccccgtggac acgtccacgg aggaaaatcc ttacacggga
     2461 ggactgggat cctacacgct cccgctgatc tgcgtgatcg atcccgggaa tcccctcgcc
     2521 tacctcgacc gcacgacggg catccaatac gagccgacct tcgtcccccg cctgctgggg
     2581 catctcccgc cggtcacggc ggccgtggag cggattgaat ggacagagaa cggcgcgctg
     2641 cgcgtcgtca tgtccggcgc gaccggcttc agctaccgcg tgctttccgc cgacagtctg
     2701 gcgggctcgt ggaggaccaa cggcgcggcc gccgccgggc cggggggcgt ttgcagcaac
     2761 gacctgccgc cgtccgcggc aggcgggttc ttcaaggtca tcgggttccg ctgacagcgg
     2821 cgactactgg gcgcggaccc ggaagaagaa gttggaatat cccgtcagca ccttcgacac
     2881 gtccacggtc gccgagcatt gcccgtcgaa tcccggcagc ccgccgtagt tccgcacggc
     2941 ccgccagacg gaccccggcg tcaggttggt cgtgtactcc agggaataca ccagcccgtt
     3001 cgtgaccggc cactggaaca ggccctccgc gccggtcacc gcgatgtcca ggtccgggat
     3061 gagatccctc ttcgcgtcgt agggctcgat ggccaggtcg tccacctcgg cctcggcgaa
     3121 atcgttggtg gtccgggtca gataattata gggattgagg ggcagcacta ttcggaaatc
     3181 gcccgtgatg gcatcagggg tcctgaacgc ccgctcgtag aaacgccact cgccgccggt
     3241 cgcggccagg acgggaaact cgccaagccc gagctggtag tcgttgttgg aatcgttgtg
     3301 gcccagtcgg ggacacagcg tgccggatgc gacgcggtac cagaagctca agcgatacca
     3361 acgccccgtt gcgacgggga cgaaacgctg ctgcaggccg ctgcggttgg cgatccagtt
     3421 ggtcccactg aatgtccgga aatggagcga ttgcgcgccc gcgtgcggat cggccgcgct
     3481 tttctccttc aactcgggcg tgccccagcg ccaccactgg ccaagttgag cgttctccat
     3541 gtcgccgtcc aggacgatcc gcgcatcctg cccggccgtg atctccacgt cgtcggccca
     3601 ggcctcgccg gcgctggcga ccagggccag gcggaagtcg tgggtaaaac cggcgggcac
     3661 ggtgaaccgg cgctccatgg cgttccaggt cccgtagttg gcggcgctca aatcatagta
     3721 ccggttctcg aagtcgctgt tggaatcgcc gatgcccagg accacgcgca acgtgccggt
     3781 cttcaccttg gccgacaagc gcagcgtgta ctcggcgccg gcggtcacgg gcagattcaa
     3841 ctgctgcacg ccgccggtcg ccacgtggag aaccgtcgaa ccgcgttccg cgtcgaattc
     3901 cttggccttg acgaacggca cgctccagtc cagccatgca ttcgtgtccg ccgcctccat
     3961 gtccccgtcc tggacgattt gcgcggacgc gcaccaggac atcattataa aaccgaatac
     4021 cgcgattgcc tttttcatgc ccttctccac tctcgattcc gcggaccgat acgggccggg
     4081 acttcagccc tgtagtcgcg ccgcgggatt cctttcgtcc cgcccgcggg agacgcatgc
     4141 tgcaatcaac cgggggataa agagatacgg ctcgcccgaa cgcttcgcgg ggccggctta
     4201 ccgaacgacg tcgaggatgg gccggtcgcc gatgtagaga ttctcgatgg ccgcgcgccc
     4261 gcgccagacg cggacggcca gccaggcgtc ctgcgcgtcc tgccggctgt gctcgcggta
     4321 ggcgcgctcg gcctcggggg cctgtcgctc gctcatgtag aaccggtcca gcggaaggtt
     4381 gagccaggct ttttgttcct gcacgttgaa cgaggcaatc ctcgtgcgga tcgccggcgc
     4441 gccggccggc ggccggcgat gtaaccggac gaaccgggcg aacccgtcct cgccgacggc
     4501 caactccgcg aaaaaccagc cgccttgctt gcgcgtccag gggccttcaa acggcgcggt
     4561 gttctgctcc acccggacgg ccacgtagcg gccgcggaag gcgtccacgg gatccatcgg
     4621 cgccgtcttg aacttgtaca catggccaag gcgcaggatc agctcgcgtt cggcgatctt
     4681 ccaggtcgca gcgacccact gcgcgacaca ggcggcgagg aagaggacga gcaggacgcg
     4741 gcggttcatg gcgcgcctcc cttccgccgc agcatgatcc agttggcgcc gagaaaaccg
     4801 agccccagga ggataaagac aatgccttta gccacgaagg acaggtccga gtcgaaaaag
     4861 cgtatgatga tgagggcgcc caggatcaac atgccgccgt tggtcttgcc caactgccgc
     4921 tgccgggcgc cggacaccag caggaacaga ccaagcccga acaggtagcc gttggcgatg
     4981 agccagcccc gcccctggcc cgccagcacg ccgatccaca cgagcagcgg aaagactccg
     5041 aataccatcc ggcccccgcg cccgcccagc acggcccgcc ctgccgtcgc gaggtacagc
     5101 agcggaaggc acacgccgag caggacgccg gacaggccgc cgcccacgaa cgccgcgtat
     5161 tcctggaatg agagccgccc ggcctcccac tcgaagctca agatgaaggc catgatcacg
     5221 atgccgccgg cgcccagcag cttcgtcggg cgctgccagg ccgtcggcgc cccttccagc
     5281 cagagcgcgt cggccaggta gaacgacgcg agcagcaggc tgaacatcag caggagatac
     5341 tcgtcttcct gttcacccgt aatgaccccg gacaggatca gcaggctgac cgccagcccc
     5401 cacaacagca gcgcggcgcg ggcctggtac cggtcttccc ggatccgggc ccacaggaac
     5461 ggcagcaaga gggcgatcag aagccagtag cccgtcggcc cgcaggtctc cagggccatc
     5521 aggctccagg aggaaacccc ggcgagatag aacacggccg gcaggaccgc gcgatgcgtg
     5581 tagaccagcg gcaaaccgag cagcatccag gtcaggatga acgactccat gtcggctgcc
     5641 acgtgatacg tctgggcaat cagggccagc gacgcgcccg ccaccagcat gagaaacgcg
     5701 gaggacgcct cccgcaggcc cgcggccgca gcctggcgaa cgaccgcgcg gcccgccatg
     5761 acctggccga acagcaggag ggcgagggac atccccgcgc gcacgggacg ggatatctgt
     5821 tcccagttgt gcgccagtag gaggatgatg ccgaacccga tcagcacggc gcccaggatc
     5881 gcgaagagcg tcagcagcca cccgcggccc gcggccggtt gggcggagcg atagtggcgg
     5941 cgcaaggatt ccgcctgctc cggcgtcagg acgccctggc ctacccatcc gggcaactcg
     6001 ccgaggagcc actggatgtt gcctttgcgg gtcatgggag gattcaggat tcaggggaag
     6061 aacaggcaca ggatgacttt tcgccggaga gacccttgca gctgcaggag ccatggcggc
     6121 ggttgcggcg agtcaacgcg aatccgagcc cgacaacgca cagcgccacg atgcccagca
     6181 cgatgcccag ttgcacgagc tgattcatga ctcgctcttg atcatgacta gagccatttt
     6241 aaaaggcttc gcggccttga gggcgtgcgg ctggttcgcc ggcatgatga ccatgtcgcc
     6301 ggtcttcgcc cgcagcccct ttccggaaat cgtgatgcgc gcctcgccgt caaggatcca
     6361 gaccatcgcg tcatacggcg cggaatgggt gctcaaaccc tgccccttgt cgaacgcgaa
     6421 cacggtcacc gtgccggcct tcttggagag gatcgtgcgg ctgactacgg cgccggcctg
     6481 gtagtccgcc agcccggcca gcggtagcgc cctggacatc aactcgcctg actgtttccc
     6541 tgttttagtt gacgccatat tcgcccgtcc cttctccaaa catttttccc tcaaataagc
     6601 atactatacc tgatcgcccg cggctggtac aaaaagaggc tgatgaaacg gctgcttcat
     6661 tcgagcctgc tcttcgcgct gctggccggc caccccgcgg ccggcgaatg gcaggacaag
     6721 gtggacccgc gcgtgctgca atccgttgac ggctccggtg atgcgcaaac ggaattcctc
     6781 gtttttctcc aggagcgcgc ggacctgtcg ggcgcggacg ccttgcgcac gaaggatgaa
     6841 aagggccggt tcgtgtaccg cgcgctaacc cagaccgccg cgcggacgca gccggccctg
     6901 cgcgcgcgcc tgcaaacgct gggcgcaatt caacaagcct tctggatcga caatatgctc
     6961 ctcgtgcacg gcaacgcgtc cctggtccgc gagttggcgg agcggcccga cgttcgccgc
     7021 ctgtccgcga atccctgggt ggacgcgcgc ctgccgcgcg accgggcggc gccgcgcgcc
     7081 ccggacgcgc ccgcggctgc ggagtggaac atcagcaaga tcaacgcgac cggcgcctgg
     7141 gccctgggct tcaccgggca gggcgtcatc gtcggcggac aggacaccgg ctacgactgg
     7201 gaccatgcgg ccctgaaggc ccagtatcgt ggttgggacg gcgagagcgc cgaccataat
     7261 tacaactggc acgacgccat ccacagcggg ggcggcggct gcggaccgga ttcgccggtc
     7321 ccgtgcgacg acggcaccca cggcacgcat accatgggca cgatggttgg cgacgacggg
     7381 accaaccagg tgggcgtcgc ccccggcgcg cggtggatcg gctgccgcaa catgaacgtc
     7441 ggctggggca ccccggccac gtatgccgag tgcttccagt ggttcgttgc gcccaccgac
     7501 ctggccaacg ggaaccccga cccggccagg gcgccggacg tgatcaacaa ctcctgggga
     7561 tgccccaccg ccgagggctg cacggacccc aacgtcttga gcggcgtggt cgccgccgcg
     7621 cgggccgcgg gcatcgtcgt ggtggtctcg gcgggcaaca gcggctcgtc ctgcggcacg
     7681 gtcagcgatc cgccgtcctt ctacgaggac tccttcaccg tcggcaacac ggactccagc
     7741 gacaacatca acagcggcag cagccgcggg ccggcgacgg cggacggcag cgggcggatg
     7801 aagcccgaca tcagcgcgcc gggcaccagc gtccgctcct gcgtgccggg caccggattc
     7861 ggctacaaga ccggcacgag catggccggg ccgcatgtcg cgggcacggt ggcgctggtg
     7921 ctctccgcgc atcccgggtt gcggggccag gtggaccgga tcgagaaact gctcacgcgc
     7981 acggccctgc cccgcaccac gacccaggta tgcggcggcc tgtccgggac gaacgtgccc
     8041 aacaacacgt acggctgggg ccgcgtggac gcctaccggg ccgtggggct cgacgatgac
     8101 gacgcggacg gcatgccgaa ctggtgggaa atcgtcttcg acctcaaccc gacaaacgcc
     8161 gccgacgccg gcttcgatcc cgacggcgac gggctgctca acgggcagga attcagcgcg
     8221 aacaccgatc ccacgaacgg agcttccgtg cttcaactga tcgaggccct gccttcggaa
     8281 tccaacgccg tggccgtcgc gtggcagagc gggcaggaag gattcgattc cggccgcacg
     8341 tacgatctct accggtcgac cagcatggcc gacggcgacg gctggacctg cgtggcttcc
     8401 aacatccccg tcgcgggcgc gctgaccggg ttcacgaacg tgtgggattc gggcacggtt
     8461 ccccaagcct actaccgcgt tgccatttcc ggcgcgagca acgaggtctc gtccgcgccg
     8521 ataggcgtcg tcccccgccc gtaatgcgag gtccatggag ggcggatctc cgcgacgccg
     8581 cgtgggctcg cgggagttcg ctcctccatc cagcttctta tggcgcaaac cggctggctt
     8641 tttacctgtc cgctggtatc gtgaccgttt gcgcggagag gaacaaatgg acttgctggc
     8701 cagaattcta cagggcgttt tcgtgatgga cggggccatg ggcacccaga tccaggcccg
     8761 cgccgtgccg gcggacgcct ggcagggccg cgacgggtgc aacgaattgc tcaacgtcac
     8821 cgccccggac atcatcgggt ccatccatcg cgcctatttc gaggccggca gcgacgccgt
     8881 cgagacgaac acgttcggcg cctcgcccat cacgctcggg gaatacgaat tgagcggccg
     8941 cgcgcgggag atcaaccggg cggccgcccg gctggcccgc gaggcggcgg cttctttcac
     9001 ggcccctgaa aaaccgcgtt tcgtcttcgg ctccataggg cccggcaccc ggctgccgac
     9061 gctcggccag gtggggttcg acgaactgcg cgcggcgctg cgggtccaga tcgagggcct
     9121 gctcgaaggc ggcgcggatg gcatcctgct ggaaacctgc caggacctgc tccagatcaa
     9181 ggcggggctg gcggcgtacg acgacgtcgt cggccgctcg gcgaaggttc cgctgtacgt
     9241 ctccgtgacc gtggagcaga ccggcacgct gctgatcggg tcgtccatcg ccgccgtcgt
     9301 ggccgcgctg tcgccttacc ccatccacat cctgggcctc aactgcgcga ccgggcccga
     9361 ggcgatgcgc cttcacctgg actacctggc ggcgaactgg ccccgcctcc tggcctgcat
     9421 gccgaacgcc ggcctgccgg agatgaaggc cggcggggtc cgctacccgc tcggacccgc
     9481 gttcttcgcg gagaaactcg gcgcgatggc ccgcgaggtc ggcctgaacg tcgtcggcgg
     9541 ctgctgcggc acgacgccgg accatatccg cgcgctggcc cagggcctgc gcgggttcca
     9601 ggccccggcc cgcgccgcgc gcgcgccgca gcaggccgcg agcgtgttct cgccggtgga
     9661 cctgacgcag gacccgccgc cgctgtacat cggcgagcgc gcgaacgcca cgggctcgaa
     9721 gaagttccgc gacgccctgc tggcggacaa gtacgacgac gccttcgccg tgcttccgga
     9781 gcaggaggag gccagcgccc acgtcctcga cttgagctgc gcctacgcgg gccgcgacga
     9841 gaagaaggac atggagatcc tcgttgcgcg cgccgcccgc gagtgccgcg ccccgctgat
     9901 gatcgattcc acgcaggccg atgtcaccga ggccgcgctc aagctctacg gcgggcgcgc
     9961 gctggtcaat tccatcaact tcgagtccgg cgaggaacgc gcgcggcacg tcgccgggct
    10021 cgcgcggaaa ttcggcgccg ggctggtctg cctgacgatc gacgagaccg gcatggccat
    10081 gaccgcggac cgcaaggtcg ccatcgcgcg gcggctggtc gagttctgcg aggccgaggg
    10141 catccggcgg gacgcgctgt tcgtggacac gctgaccttc accatcggca gcggcgaccc
    10201 gacgctgcgc acagccgccc tggagaccct ggaggcgatc cgccggatca agaaggaact
    10261 gccggacgtc cggacgatcc tcggcctgtc gaacatctcc ttcggcctca agcccgcggg
    10321 ccggaaggtg ctcaacaccg tctttctcga ccgctgcctg aaggccggca tggacgcgtg
    10381 catcatcaac gtcgcgtcca tcgtgccgct gacggacatc gcgcccgacg ccctgcgcgt
    10441 ggccggcgcg ctgctggaca acgacgcgtc ggcgggcgac ccgctggaga acttcatacg
    10501 gttcttcgag ggagccgtcg acgcgggggc ggaggccgcc gcggccgccc tgccgccggc
    10561 ggaagcgctg gcccgggccg tgatcaaggg acggccgcag ccgctggagg ccgcgatccc
    10621 cgcactgctc ggccagcacc cggcggaatt catcctgaac gagatcctga tcccggcgat
    10681 gaaggaggtc ggccgcctgt tcaacgacgg catcctccaa ctgcccttcg tgctgaaatc
    10741 cgccgaggtc atgaagaaag ccgtcgacct gctcaagccg cacatgaaga aggacgagtc
    10801 cggcgggcgg cgcggcctga tggtcctcgc caccgtcgcg ggcgacgtcc acgacatcgg
    10861 caagaacctc gtggacatca tcttgagcaa caacggcttc cacgtcgtca acctgggcac
    10921 gaagatcccg atcgagcgga tgatcgcggc cgtgcgcgag cacaaggccg acgtgctggg
    10981 aatgagcgga ctgctggtga aatccgccgc gatcatggcg gagaacatga aggcgctgga
    11041 ggccgccggc atccggatcc ccgttttcct gggcggcgcg gcgctgacgc cggggttcgt
    11101 ggccgactcg tgccagcccc gctactccgc gcccgtcgtg tactgcaagg acgccttcga
    11161 gggcctggcc agaatgcgcg agttgtccga gaccggccgc ctggaaaaag cgcaaccgcg
    11221 gccacccagc gcgccgcccc ccgatttcga ggcgcccccg gtatcgattg atctctcgtt
    11281 gccgccgccc aagccgccgt tcctcggcca ccggatcacg gagagcatcc ggctggagga
    11341 cgtgtatccg cacctgaacg agactgcgct gatccgcggg cgctggggtt accggcgcgg
    11401 caagatgggc gcggaggaat acgaccggct gatcgaggac gaggtgaagc cccgtctcga
    11461 agcgctgaag aagcagtgcg ccgagtcggg cctcttccgg ccgaaggcgg cacacggctg
    11521 gttctggtgc cgggccgaag gcgaggcgct gacggtgttc ccctcggaag gcggagaacc
    11581 gatccgcatg gtcttccctc gccaatcccg ttcgccgggc ctttcgatcc ccgatttctt
    11641 ccggcgcgac gaggacgtga tcgggtttat ggtcgtgacg ctcgggcccg ggctcgagac
    11701 cgagaacgcc cggctgctgc agcgtgatca ttaccaggac tacttcctgc tgcacggcct
    11761 ggcggtcgag gtcaccgacg cgctggccga gtactggcac gcccggatgc gcgcggagtt
    11821 gggcttcacc gagccgaagc tggcgatcca ggattacatt gtgcagaaat accggggctc
    11881 gcggtacggg ttcggctacc cggcctgccc ggacctctcc atgaacaagg tctgctgcga
    11941 cctggtccac gcggccgata tcggggtgag cctgtcggac aatttcatga tggtgccgga
    12001 ggtgaccact tccgcgctcg tggcgcacca tccagcagcg aagtacttca acgtctgacg
    12061 gcggaagtga acgcatcacc gcggcaggtt gtctcaacgc tcatgacaag cggaagccac
    12121 acgtcgccgc ctgtcttctg gctggtttcc ggaatttccc ttctcctgta cacgatcaca
    12181 ctggctccct cggttacgct ggagtactcg ggctcgctcg tcacggcagc cgacagcctc
    12241 ggcgttgcca acccgccggg ctaccccgtg tggaccttgc tggcctggtt cttccagaag
    12301 ctcttcggct tcgtccgcta tcacggcgcg cccaacccgg cgtggggcgt taattttatg
    12361 tccgcgtttt tcggcagcct ttcatgcggg ctggtcgcgc acatggtcag ccgctccgtt
    12421 cggtccgaat cccggggggt ctccgatcca caggcgagct gcgcggcttg gatcgccggg
    12481 acgtcggccg gcctcctgtt cgcgttcagt ccattcatgt gggcgcaatc ggtcatcgcg
    12541 gaaaccaata cactgaacgt gttctccgtg gttttgttcc tcgcgctgct acagggcggg
    12601 gtattggatg acgggaaacg ttttctctat ccgctggcct tcctgctggg gatcgggttc
    12661 ctgaacagcc ccaccttcgt gctgatgatt cccctgtttg cagccgcctg ggccaagcgg
    12721 gggacgtgga ggcaggtccc cgcgctcctg ctgtgtctgg cgttggggct cctcccgatg
    12781 gcctacctcc ccctcgcctc tgcgcagaat ccgccggtca actggggcta tgcgcggacg
    12841 ctcgacggat tcatccacct cgtcacgcgc gggcagtacg aacacatgac gctgacgccc
    12901 gttttcagcg ctcccgacgt cttcctagct cagatggcca cctatatccg cctgctggcc
    12961 gcgcagttct cgacaccggt gctggtcctc ggcatccccg cgttgttcgg catgcggcat
    13021 ggcggcctgt ggaagccggc cttgctggcg gccttcctgc tctacggcac gggcacgacg
    13081 atcggcatga atccaccgct cgaccaccag acgctgtact attgccgcgt ggtgtggata
    13141 ccctcctatg ccctcgccgc catcctcatc ggacttggac tcggatggat cttc
//
LOCUS       DAOOTK010000093        13172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3152_length_13172_cov_4.482351, whole genome shotgun sequence.
ACCESSION   DAOOTK010000093 DAOOTK010000000
VERSION     DAOOTK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_13172_cov_4.482351"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="PK634_10330"
     CDS             complement(<1..123)
                     /locus_tag="PK634_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98172.1"
                     /translation="MNLTRAAHTLRGLAIDGIQKANSGHPGMPLGAADMAAVLFL"
     gene            complement(218..622)
                     /locus_tag="PK634_10335"
     CDS             complement(218..622)
                     /locus_tag="PK634_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98173.1"
                     /translation="MDQPASRKPRRHDKVEISFLEAIHRRCGQNRRVWQALGDLYTRT
                     GRFEDGLAMDLRMAAACPDDPLAQYNLACSYALTARLEEALEALERAVTAGYRDVDWM
                     SRDRDLKSLHGDPRFRAILRRLKGRARPAPPA"
     gene            complement(640..1938)
                     /locus_tag="PK634_10340"
     CDS             complement(640..1938)
                     /locus_tag="PK634_10340"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOW98174.1"
                     /translation="MSGEAESGKAQLAFDGRTVDLPVLTDHIGQRAVDIRNLRKDAGL
                     TAFDPGFANTVSCRSGVSYVDGEAGRLVYRGYAIEDLADNCTFIEVAYLLLHDRLPTR
                     PQLEQFSVLMNRHSMIHEDMITFFAKYPEHAHPMAVLSAMVVSLSSFYPELEESESEE
                     LDITATRLLSKLRTIAAFSYKKSIGEPFVYPSYKLSYCANFLNMMFSSPVRDYPMDPM
                     LVRAMNQLLILHADHDQNCSTSTVRLVGSARVNLYACIAAGICALWGPLHGGANQEVI
                     EMLELIHKQGGNVDGVIEKAKDRKDPFRLSGFGHRIYKTYDPRAKIAKRICQEVLGKL
                     GVSDPLLDIAMRLEEKALADPFFVERKLYPNVDFYTGITYRAIGFPTDMYTVLFALGR
                     LPGWIAQWQEMVRDPEQKIWRPRQLYTGQNNLPFVPIDKR"
     gene            complement(2067..2294)
                     /gene="rpmB"
                     /locus_tag="PK634_10345"
     CDS             complement(2067..2294)
                     /gene="rpmB"
                     /locus_tag="PK634_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOW98175.1"
                     /translation="MAIACEICGKGARTGSRIVRHGLAKKKGGIGLHTTAVTGRRFLP
                     NLQRLRVRENGGVKTRRVCTACIKAGRIVKA"
     gene            complement(2388..5063)
                     /locus_tag="PK634_10350"
     CDS             complement(2388..5063)
                     /locus_tag="PK634_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOW98176.1"
                     /translation="MADDQPVNGADKVETYRYRLPLGVNLAHYRILRPLGYGEFGEVY
                     LAEDLETRKRSALRIMPDELAAQPGTASTLRETVQVSEKLDHPAIRAIRETGTDQGLM
                     YLAMDYIEGPHGQPRALRDEIRDGGGRIRDERKVRHIALQICEALEHAHAAGVVHGYL
                     HPSDILIGSLDAHQTRHRRLGAGGPSALDDLEVHVSDFGMMPLREAMSEAVKSDAYTS
                     PEENAGQVTPQTDIYAFGVILYQMLTGGRPGPTFVPPSRYGASKVWDPIAERCLEPDL
                     ANRYASATELKRDLEKAVTHRALRRMLVALALLALAGVAAWQGYRVWLKVQAAIERRA
                     ELRRLEAQRRAEAHALVQEARDLFSLRKFPEADVLARQALALVPDQPEALALQKEIVK
                     AGDKVQAELARDDGTRKMDRLRGDRLEEAQKDALAEAEKLFASAEARFKAGDYAMAKE
                     TFRGAVAEADRGLKLGEDRQAALKNKQRAEQARWNAKRHSAERDDPETWANAEDRFAA
                     AVRALDTGLYFDASREWQASIQLYGQADQVGLAAMKARAEEARSVQAQEEERQMHLRW
                     PGLPDGYRVVGQETDAASGLPKVIESKKSAYRLLLIPAGAFTLGRPAGSGGEDEHPAR
                     RIELGDFWIGESEVTVEQYTGFLIENGNQTEDGVTWANLGPAIKLRMVEGQFMPEPGF
                     EEHPVVEVSWYGARAFAEWIGGELPTEAQWEKAAKGGKDWLWPWGDEWDWSRANTAER
                     LAGQVEFKGYAEWLAWWEPFQRAVLARSKKAYEETTRPVRSFEPNGYGLFDMAGNVLE
                     WCRDYYVPQAYRDLADGQKDPPPVPQGSPEKVTYWRGGEKFEETAECRVVRGGSWGYM
                     GNQARCAFRFKALSTGRYSDGGFRVVVSPRRAAVP"
     gene            complement(5093..6727)
                     /gene="pgi"
                     /locus_tag="PK634_10355"
     CDS             complement(5093..6727)
                     /gene="pgi"
                     /locus_tag="PK634_10355"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOW98177.1"
                     /translation="MPRRRKQLTTYSEWKALQSHRRDMQGRSLRELFAEDAGRAARFS
                     LEAGDLYLDYSKNLVTAETMTLLLALARAVDVPGAVADMFAGRKINETEDRAALHVAL
                     RNRSNIPIRVDGRDVMPEVNAVLERMAGFARRVRSGEWKGFTGRPIRNVVNIGIGGSD
                     LGPVMAYEALKPFSERALTLRFVSNVDGTHIAEATRDLDAAETLFIVSSKTFTTQETM
                     TNAQTARDWLLRALGDPAAVGRHFAAVSTNADKVTAFGIDPDNMFGFWDWVGGRYSLP
                     SAIGLSLMIAIGPERFVELLEGYHVMDRHFAEAPPERNMPVLLALLGVWYNNFFGAET
                     VAILPYDQYLARLPAYLQQADMESNGKSVSRRGKPVRCQTGPIVWGEPGTNGQHAFYQ
                     LIHQGTKLVPCDFIGFCRSQNPLGDHHAKLMANFFAQTEALAFGKSAAELAAEGVAPR
                     LVPHKTFGGNRPTNTILADALTPRVLGQLLALYEHKIFTQGVIWDIYSFDQWGVELGK
                     ILANRILPEIQSETEPELRHDSSTNALLRRFRGRRG"
     gene            complement(6854..7657)
                     /locus_tag="PK634_10360"
     CDS             complement(6854..7657)
                     /locus_tag="PK634_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOW98178.1"
                     /translation="MGGPFVTLLTDFGTGDGYVAAMKGVLCSRAPRATVVDAAHDIPP
                     HDVAAAAWTLGQYWRYFPPGTIHVAVVDPGVGTERAILLVEADSHLFLAPDNGVLTWV
                     LRQARRVALRRLKAAVHGETVSTTFHGRDILAHAAGLLAAGHATVEQLGEDIRELVMP
                     AWAAVERVGDGLRGQIVHVDRFGNLITGIQRHQALESVGAAFTATAGGFRDIPGRRTY
                     ADAQPGELIALFGSSDTLELAVNGGSAAERTGLGRGQAVVLHPALKIEG"
     gene            7774..8346
                     /gene="folE"
                     /locus_tag="PK634_10365"
     CDS             7774..8346
                     /gene="folE"
                     /locus_tag="PK634_10365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOW98179.1"
                     /translation="MNKESMENLTRQLLVELGEDPGREGLRRTPERVADAWSFLTRGY
                     QQDVHSVVQSAIFEVEANHMVIVKDIEIYSLCEHHLLPFFGRCHIGYIPKGRVFGVSK
                     LARVADIFARRLQLQERLTNQIARVMMDALGPEGVGVVIEARHLCMMMRGVEKQNSCM
                     VTSAMLGSFHDSIATRNEFLQLVGHEARGV"
     gene            complement(8356..9276)
                     /locus_tag="PK634_10370"
     CDS             complement(8356..9276)
                     /locus_tag="PK634_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOW98180.1"
                     /translation="MKDKDAIQLVVVGSTAIDNIETPWAKRKNLLGGSASYACAAASF
                     FTTVGMVGVVGEDFPKEHMELYRHFGIDLEGLQIKPGRTFRWAGAYEENMDQRRTILT
                     ELNVFATFRPDLPEPYRAAPFLFLGNISPALQAHVLDQVQTPKFIVLDTMDLWITTEV
                     EALRKVIGRVHLLTLNESEARHLSGKHSLIKASKRLLELGPKYVLIKKGEHGSVLFTR
                     EGMFLMPAYPLEDVQDPTGAGDAFAGGFMGALAAGGYVGEDAIRRAMLYGSIVASFGV
                     EAFSLERLGMLSREEIEARASLFRNMCQVR"
     gene            complement(9314..9880)
                     /locus_tag="PK634_10375"
     CDS             complement(9314..9880)
                     /locus_tag="PK634_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOW98181.1"
                     /translation="MNWDRMAEDLLAVVEEVIGGLRGPVEELASEIAARLRAGGKVLA
                     CGNGGSAADAQHLAGELMNRFLKERRPYAGIALSTDTSVLTSIANDYSFAEVFEKQVR
                     ALGRRGDVLIAISTSGNAENVCRAVKAAKDLGLLTVGLTGGSGGKLRPLVDRPLCVAS
                     TAATPRIQEGHQLIIHALCERLEELLES"
     gene            complement(9877..10449)
                     /locus_tag="PK634_10380"
     CDS             complement(9877..10449)
                     /locus_tag="PK634_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98182.1"
                     /translation="MKPLRILSGVLLFLFLVAAGAAVIAAARSPEDWRQAMDLVRSER
                     WLALGAAAAVLCALAVYLLAGLKPRRYSEPFITFENDSGSVSVSTRAIGEVVSRVGDE
                     FAAVLGLDSAVRPAGGSIEVSLDVRVRGGTQIPELCRMMQDRVREIIRENLGLTEIRG
                     VKVTVREIVASARGVAEEGAGRRPGAAEPA"
     gene            complement(10446..10922)
                     /locus_tag="PK634_10385"
     CDS             complement(10446..10922)
                     /locus_tag="PK634_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOW98183.1"
                     /translation="MSEATPAENNATMDPRHPLRAAGAAVPPDDNAEEGMDQGEIKIH
                     HHVIATISRLAALKVPGVAALSPSFAQGLAKVFGRKMDDTGIRVLLLETGVVIDLYVV
                     VHFGVRIPQVAWQVQSEVRQAVEQMTGKNVKSVNVVVQSLQFGAGQNPAREEGKAP"
     gene            complement(10941..12305)
                     /gene="accC"
                     /locus_tag="PK634_10390"
     CDS             complement(10941..12305)
                     /gene="accC"
                     /locus_tag="PK634_10390"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOW98184.1"
                     /translation="MFERILIANRGEIALRIIRACREMGIKTVAVHSEADESSLHVQM
                     ADESVCIGPAPAKESYLKIANIISAAEVADVDAIHPGYGFLSENAHFAEICENCKITF
                     IGPSADNIRMMGDKAKARETMKKAGVPVTPGSEGVIANQDEALKAARQIGYPVLIKAV
                     AGGGGKGMRIARNDVSLVQSFMTAASEAERAFGNPEVYIEKFVEKARHIEVQVFGDHH
                     GNVVHLGERDCSLQRRHQKLIEESPSPAISPDVRKKLTRAAVRGAEAVKYRNAGTIEF
                     LFDVEASQFYFMEMNTRIQVEHPVTEEVAGVDLIKEQISVAAGNKLSFSQKDLAFAGH
                     AIEVRINAENPYKNFTPSPGRIQFVSMPGGPGVRVDSHVYTGYEINPHYDSMIGKIIV
                     RGRNRAEAIAKMTRALSEFVMEGPATTVPLCQALLGDTRFARGEYTTAFLESFMQDVF
                     LITP"
     gene            complement(12340..12825)
                     /gene="accB"
                     /locus_tag="PK634_10395"
     CDS             complement(12340..12825)
                     /gene="accB"
                     /locus_tag="PK634_10395"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOW98185.1"
                     /translation="MDILTIKQIVALMKDNDLSEFEVEEKGLRISIKRSLGGEPRVAI
                     SALPVASPPVVVSAAATAPGVAPAPAPAAPAEVPEESKWTPVKSPIVGTFYRAASPDA
                     EPFVTVGQQVEPDSIVCIIEAMKVMNEIKAETRGVVKKILVDNASAVQYGQTLILIDP
                     A"
     gene            complement(12923..>13172)
                     /locus_tag="PK634_10400"
     CDS             complement(12923..>13172)
                     /locus_tag="PK634_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98186.1"
                     /translation="LEARAGIVRCLMEWRRDALLLVLGLEPGDLHFPEEADALRRQAA
                     GLDYARALRRFRAVETLHRQLERNIPDELAFEHLWLEG"
BASE COUNT         1941 a         4677 c         4351 g         2203 t
ORIGIN      
        1 caggaacagg acggccgcca tgtcggccgc gccgagaggc atgcccgggt gcccggagtt
       61 cgccttctga atgccgtcga tcgcaaggcc gcgcagggta tgggctgccc gtgtcaggtt
      121 catggtcttc ctttcgcgca aaaaacctgc ccactatagg cgcgaacggc gcgcggcgca
      181 aggggggcgc ggcgttttcg atctccagcg ggcttcttca ggccggcggc gccgggcgcg
      241 ctcggccttt cagccggcgc aggatcgcgc ggaagcgggg gtcgccgtga aggcttttca
      301 ggtcccggtc ccggctcatc cagtcgacgt cgcggtaccc cgccgtgacg gcccgctcga
      361 gcgcttccag cgcctcttcc agccgcgcgg tcagggcgta gctgcaggcc aggttgtact
      421 gggccagggg atcgtccggg caggccgcgg ccatccgcag gtccatggcc agcccgtcct
      481 cgaagcggcc ggtgcgcgtg tagaggtcgc ccagcgcctg ccatacgcgg cggttctgtc
      541 cgcagcgacg gtggatggcc tcgagaaacg agatctccac cttgtcgtgc cggcgaggct
      601 tgcgggaggc gggctggtcc atggcggctc cgggcggggt caccgcttgt cgatgggcac
      661 aaacggcagg ttgttctggc ccgtgtagag ctggcgcggc cgccagatct tctgctccgg
      721 gtcgcgcacc atctcctgcc actgggcgat ccagcccggc aggcggccga gcgcgaacag
      781 cacggtgtac atgtccgtcg ggaacccgat ggcccggtac gtgatgccgg tgtagaagtc
      841 cacgttcggg tagagcttgc gctcgacgaa gaacgggtcg gcgagtgcct tctcctccag
      901 ccgcatggcg atgtccagca gcggatcgct gacgcccagc ttgccgagca cctcctggca
      961 gatccgcttg gcgatcttcg cccgcggatc gtacgtcttg tagatgcggt gcccgaagcc
     1021 gctcagccgg aacgggtcct tccggtcctt ggccttctcg atcacgccgt ccacgttgcc
     1081 gccctgcttg tggatcagct cgagcatctc gatgacctcc tggttggccc cgccgtggag
     1141 cgggccccag agggcgcaga tgccggccgc gatgcaggcg tagaggttga cccgcgcgct
     1201 gccgacgagc cgcacggtgg acgtgctgca gttctggtcg tggtcggcgt gcaggatcag
     1261 gagctggttc atcgcccgga cgagcatcgg gtccatcggg tagtcccgca cgggcgagga
     1321 gaacatcatg ttcaggaagt tggcgcagta gctcaacttg tagctggggt acacgaaggg
     1381 ctcgccgatg gacttcttgt aggagaacgc ggcgatcgtg cgcagcttgg agagcagccg
     1441 cgtcgccgtg atgtccagct cctccgactc ggactcctcc aactcgggat agaagctgga
     1501 gagcgagacc accatcgccg acagcaccgc catgggatgc gcgtgctccg ggtacttggc
     1561 gaagaaggtg atcatgtcct cgtggatcat cgagtgccgg ttcatcagca cgctgaactg
     1621 ctccagctgc gggcgcgtgg gcagccggtc gtggagcagc aggtaggcca cctcgatgaa
     1681 cgtgcagttg tccgcgaggt cctcgatggc atagccgcgg tacaccagcc ggccggcctc
     1741 cccgtccacg tagctgaccc cgctccggca gctgacggtg ttggcgaagc cggggtcgaa
     1801 cgcggtcagc cccgcgtcct tccgcaggtt gcggatgtcc acggcgcgct gcccgatgtg
     1861 gtccgtcagc accggcaggt ccacggttcg cccgtcgaac gccagttgag ccttgccgct
     1921 ttccgcttca ccgctcatgg tcgcctcccg cgttaccctt tcatattgcc gggtccggga
     1981 tcgaatgcaa gcctgccccg ccgcgccggg aaaaaaagaa gagcccgacc cggccgggtc
     2041 gggctcgaga ttctcggacg gaaggatcag gccttgacga tccggccggc cttgatgcac
     2101 gccgtgcaga cgcggcgcgt cttcacgccg ccgttttccc ggacgcgcag gcgctgcagg
     2161 ttcggcagga accgccgccc cgtgacggcc gtggtatgca ggccgatgcc gcccttcttc
     2221 ttcgccaacc catgacggac gatccggctg cccgtccggg cgcccttacc gcagatttcg
     2281 catgcaatcg ccatacaatg ctccttccaa gccttccaag ggcgcgcact ataggggcga
     2341 gtcccgccgt tgacaagccc aaagtcccgc agccgcagcc ggcggagtca ggggacggcc
     2401 gcgcgcctgg gggaaacgac cacgcggaag ccgccgtccg agtagcgccc ggtggacagg
     2461 gccttgaacc ggaaggcgca ccgggcctgg ttgcccatgt agccccagct tccgccgcgc
     2521 accacccggc actccgcggt ttcctcgaac ttctcgccgc cgcgccagta ggtgaccttc
     2581 tccggggagc cctgcggcac cggcggcgga tccttctgcc cgtccgcgag atcccggtag
     2641 gcctgcggca cgtagtaatc ccggcaccac tcgagcacgt tgccggccat gtcgaacagg
     2701 ccgtacccgt tgggctcgaa gctgcgcacg ggccgcgtcg tttcctcgta ggcttttttc
     2761 gagcgggcca ggacagcgcg ctggaaaggc tcccaccagg ccagccattc cgcgtagccc
     2821 ttgaattcca cctggccggc cagccgctcc gcggtattgg cccggctcca gtcccattcg
     2881 tcgccccacg gccagagcca gtccttcccg cccttcgcgg ctttctccca ctgcgcctcg
     2941 gtcggcagtt cgccgcctat ccattccgca aacgcccgcg ccccgtacca cgagacctcg
     3001 acgaccggat gctcctcgaa cccgggctcc ggcatgaact ggccctcgac catgcgcagc
     3061 ttgatcgccg gacccaggtt cgcccaggtc acgccgtcct cggtctggtt gccgttctcg
     3121 atgagaaaac ccgtatactg ctccaccgtc acttcggatt cgccgatcca gaaatcgccg
     3181 agctcgatgc gccgggcggg atgctcgtcc tcgccgcccg agccggccgg gcggcccagc
     3241 gtgaacgcgc cggccgggat caggagcagg cggtaggccg atttcttgct ttcgatgact
     3301 ttcggcaggc cgctcgcggc gtcggtctcc tgcccgacca cgcggtagcc gtccggcagg
     3361 cccggccagc ggaggtgcat ctggcgctcc tcctcctggg cctggaccga gcgcgcttcc
     3421 tcggcgcggg ccttcatcgc ggccaggccg acctggtcgg cctggccgta gagctggatg
     3481 gacgcctgcc actcccggct cgcgtcgaag tagagccccg tgtccagcgc ccgaaccgcg
     3541 gccgcgaacc ggtcctcggc attggcccac gtctccgggt catcccgctc ggcgctgtgc
     3601 cgcttggcgt tccagcgggc ctgctccgcc cgctgcttgt tcttcaacgc cgcctgccga
     3661 tcctcgccga gtttcaggcc ccggtccgct tccgccacgg cgccccggaa ggtctccttg
     3721 gccatggcat agtccccggc cttgaaccgc gcctcggcgg acgcgaacag cttctcggcc
     3781 tcggccagcg cgtccttctg cgcctcctcc agccggtcgc cgcggagccg gtccatcttc
     3841 cgggtcccgt cgtcgcgggc caattccgcc tggaccttgt cgcccgcctt cacgatctcc
     3901 ttctgcaggg ccagggcctc cggctggtcc ggcaccagcg ccaacgcctg ccgcgcaagc
     3961 acgtccgctt ccgggaattt ccgcagggag aacagatccc gcgcttcctg caccagggcg
     4021 tgcgcctcgg ccctccgctg cgcctccagc cgccgcagtt ccgcccgccg ctcgatggcc
     4081 gcctggacct tcagccacac gcggtatccc tgccacgccg ccacgcccgc cagggccagc
     4141 agcgccaggg cgaccagcat gcggcgcaac gcgcggtgcg taaccgcctt ctccagatcg
     4201 cgtttcaact ccgtggcgct tgcataacgg ttcgccaggt ccggctcgag gcaccgctcg
     4261 gcgatcggat cccatacctt ggacgccccg tagcgggagg gcggcacgaa ggtcggcccc
     4321 ggccgcccgc ccgtgagcat ctggtagagg atcacgccaa acgcgtagat atccgtctgc
     4381 ggcgtgacct ggccggcatt ctcctccggg ctggtgtagg cgtcgctctt gacggcctcg
     4441 gacatcgctt cgcggagcgg catcatgccg aaatccgaga catgcacttc caggtcatcc
     4501 agggccgaag gcccccccgc ccccagccgc cggtgccggg tctggtgcgc gtcgagcgag
     4561 ccgatcagga tgtcggacgg gtgcagatag ccgtgcacca cgcccgccgc gtgggcatgc
     4621 tcgagcgcct cgcagatctg cagggcgatg tggcggacct tgcgctcgtc gcgaatccgg
     4681 ccccctccgt cccggatctc gtcgcgcaac gcccgcggct gaccgtgcgg gccctcgatg
     4741 tagtccatgg ccaggtacat caggccctgg tcggtccccg tctcccggat cgcccggatg
     4801 gccggatggt ccagcttctc gcttacctgc acggtctccc gcagcgtgga cgccgtgccc
     4861 ggctgggccg cgagttcgtc cggcatgatg cggagggcgc tccgcttgcg cgtctcgagg
     4921 tcttcggcca ggtacacctc gccgaactcg ccgtacccca gcggccgcag gatccggtaa
     4981 tgggccaggt tgacgcccag cggaaggcgg taccggtacg tctccacttt gtcggccccg
     5041 ttgactggct ggtcgtcggc catgaaattc gcacccggtc atcccggcgg gtttatcccc
     5101 gccggccccg aaaacggcgg agcagcgcgt tcgtcgagga gtcgtgccgc agttcgggtt
     5161 ccgtttcgga ctggatctcg ggcaggatcc ggttggccag gatcttgccc agttcgacgc
     5221 cccactggtc gaaggagtag atgtcccaga tcactccctg cgtgaaaatc ttgtgctcgt
     5281 agagggccag gagctggccc agcacgcggg gcgtcagcgc atcggccagg atggtgttgg
     5341 tgggccggtt gcccccgaac gtcttgtgcg ggaccaggcg gggcgcgacg ccctcggcgg
     5401 ccagctccgc cgcgctcttg ccgaaggcca gggcctcggt ctgggcgaag aagttggcca
     5461 tgagcttcgc gtggtgatcg cccaggggat tctgcgaccg gcagaacccg atgaaatcgc
     5521 acgggaccag cttggtgccc tggtggatca gctggtagaa ggcgtgctgg ccgttggtgc
     5581 ccggctcgcc ccagacgatc ggccccgtct ggcagcggac gggcttgccg cgccgggaaa
     5641 ccgacttccc gttgctctcc atgtccgcct gctgcaggta cgccggcagg cgggccaggt
     5701 actggtcgta cggcaggatg gccacggtct cggcgccgaa gaaattgttg taccacacgc
     5761 ccagcagcgc gagcagcacg ggcatgttgc gctccggcgg cgcctccgcg aaatgccggt
     5821 ccatgacgtg gtagccttcc agcagctcga cgaaacgctc cggcccgatg gcgatcatca
     5881 gcgaaagccc gatggccgac ggcagcgagt accgcccgcc cacccagtcc cagaacccga
     5941 acatgttgtc gggatcgatc ccgaaggccg tcaccttgtc cgcgttggtc gacacggcgg
     6001 cgaaatgccg gcccaccgcc gcggggtcgc ccagggcccg caggagccag tcccgcgcgg
     6061 tctgcgcgtt ggtcatggtc tcctgggtcg tgaacgtctt ggaggacacg atgaacagcg
     6121 tctccgccgc gtcgaggtcc cgcgtggcct ccgcgatgtg cgtgccgtcc acgttcgaca
     6181 cgaagcggag ggtgagcgcc cgctcgctga acggcttgag cgcctcgtac gccatgaccg
     6241 gcccgaggtc cgatccgccg atgccgatgt tgaccacgtt acggatcggc cgcccggtga
     6301 agcctttcca ttcccccgac cgcacgcgcc gggcgaaccc ggccatgcgc tccagcacgg
     6361 cgttcacctc gggcatgaca tccctgccgt ccacgcggat cgggatattc gaccggttgc
     6421 gcagggcgac gtggagcgcc gcgcgatcct cggtctcgtt gattttccgg ccggcgaaca
     6481 tgtccgcgac ggcgccgggc acgtccacgg cccgcgccaa ggccagcagc agagtcatcg
     6541 tctcggccgt gacgaggttc ttcgagtagt cgaggtacag gtccccggcc tccagggaaa
     6601 atcgcgcggc gcggccggcg tcctcggcga agagctccct caagctccgc ccctgcatgt
     6661 cgcggcggtg cgactgcaag gccttccact cggaataggt cgtcaactgc tttcgtcggc
     6721 gcggcatggg acatcccctt tgtttcgtca gctggaacga aaaagccgcg gtcaccataa
     6781 cagtcgagcg ggcggcaagg caagacggga cgcgggagtt ctgcttccca tcggcgcccc
     6841 ggcgaccgca atttcagccc tcgatcttca gggcgggatg caggaccacg gcctgtccgc
     6901 ggcccagccc cgtccgctcg gccgcggacc cgccgttcac ggcgagctcg agcgtgtcgg
     6961 aggagccgaa cagcgcgatc agttcgcccg gctgcgcgtc cgcgtaggtg cggcggcccg
     7021 ggatgtcccg aaacccgccc gccgtggccg tgaaggcggc gccgacggat tccagcgcct
     7081 ggtgccgctg gatgccggtg atcaggttgc cgaaccggtc cacgtgcacg atctgcccgc
     7141 gcaagccgtc gcccacccgc tccacggcgg cccaggccgg catgaccagc tcgcggatat
     7201 cctcgcccaa ttgctccacg gtggcgtgtc cagcggccag caggcccgcg gcgtgcgcca
     7261 ggatatcccg accgtggaac gtggtcgaaa cggtctcgcc gtgtacggcc gccttgagcc
     7321 gccgcagcgc gacgcggcgc gcctgccgga gcacccaggt caggacgccg ttgtccggcg
     7381 caaggaaaag atggctgtcg gcctcgacca gcaggatggc ccgctccgtg cccacccctg
     7441 gatccacgac ggccacgtgg atggtccccg gcgggaaata gcgccagtac tgcccgagcg
     7501 tccacgccgc cgcggccacg tcgtgcgggg ggatgtcatg cgccgcgtcc acgaccgtcg
     7561 cccgcggcgc ccggctgcac agaacccctt tcatcgcggc cacgtagccg tcgcccgttc
     7621 cgaaatcggt cagcaaggtg acaaatggcc cgcccatggt ttcgtcccgt agcgtactgt
     7681 aacggccggc gagtgcaacg gcgttttttt ccacggcaac gcggacggga tgtggtatgg
     7741 agaggaaggc ggagcccaac ccgggagaag agcatgaaca aggaatcgat ggaaaacctg
     7801 acgcgccagc tgctggtcga actcggcgag gacccgggcc gcgaggggtt gcgccggacg
     7861 ccggagcggg tcgccgacgc atggtcgttc ctgacccggg gttaccagca ggacgtgcac
     7921 agcgtcgtcc agtcggcgat cttcgaggtc gaggccaacc acatggtcat cgtcaaggac
     7981 atcgagatct acagcctctg cgagcaccac ctgctcccct tcttcggccg ctgccacatc
     8041 ggctacatcc ccaagggccg ggtgttcggc gtgagcaagc tggcgcgggt cgcggatatc
     8101 ttcgcgcggc gactgcagct ccaggagcgg ctgaccaacc agatcgcccg cgtgatgatg
     8161 gacgcgttgg gcccggaggg cgtcggcgtg gtcatcgagg cgcggcacct gtgcatgatg
     8221 atgcgcggcg tggagaagca gaactcctgc atggtcacgt cggcgatgct gggcagcttc
     8281 cacgactcga ttgcgacgcg caacgagttc ctgcagctcg tcggccacga ggcgcggggc
     8341 gtgtagcgac ggcgcttatc tcacctggca catgttccgg aacagcgacg cgcgggcctc
     8401 gatctcctcg cgcgacagca tgcccaggcg ctcgaggctg aacgcctcga ccccgaacga
     8461 cgcgacgatg ctcccgtaga gcatggcgcg gcggatcgcg tcttccccga cgtagccgcc
     8521 ggcggcgagc gcgcccatga acccgcccgc gaaggcgtcg ccggcccccg tcggatcctg
     8581 cacgtcctcc agcggatacg ccggcatcag gaacatgcct tcccgcgtga agagcacgct
     8641 gccgtgctcg cccttcttga tcaggacgta cttcggcccc aattccagca gccgcttcga
     8701 cgccttgatc agggagtgct tgccgctcaa gtgccgcgcc tcggactcgt tgagcgtcag
     8761 caggtggacg cggccgatca ccttgcgcag ggcctcgacc tccgtcgtga tccacaggtc
     8821 catcgtgtcc aggacgatga acttcggcgt ctggacctgg tcgaggacat gggcctgcag
     8881 ggccggcgag atgttgccga ggaacaggaa gggcgcggcg cgatagggct ccggcaggtc
     8941 gggccggaac gtggcgaaga cgttgagctc ggtgaggatg gtccggcgct ggtccatgtt
     9001 ttcctcgtag gcgcccgccc agcggaaggt cctgcccggc ttgatctgga gcccttcgag
     9061 gtcgatcccg aaatgccggt acagctccat gtgctccttg gggaaatcct cgccgacgac
     9121 gccgaccatg ccgacggtcg tgaagaacga ggcggcggcg caggcgtagc tggccgagcc
     9181 gccgaggagg ttcttgcgct tggcccaggg cgtttcgatg ttgtcgatgg cggtggaccc
     9241 gaccacgacc agttgaatgg cgtccttgtc tttcatggtt ccttggtctc ccggaggccc
     9301 gcgggggccg ggttcagctt tccaacagtt cctcgaggcg ctcgcagagg gcgtggatga
     9361 tcagctggtg cccctcctgg atgcgcggcg tggcggccgt ggaggccacg caaaggggcc
     9421 ggtcgaccag cggccgcagc ttcccgccgg agccgccggt cagcccgacg gtcagcaggc
     9481 ccaggtcctt cgccgccttg accgcgcggc agacgttctc cgcgttgccg ctggtcgaaa
     9541 tcgcgatcag cacgtcgccg cggcggccca gcgcgcgcac ctgcttttcg aacacctccg
     9601 caaaggaata gtcattcgcg atggaggtca gcaccgacgt gtcggtggac agggcgatcc
     9661 ccgcgtacgg cctgcgctcc ttgaggaacc ggttcatcag ctcgcccgcg agatgctggg
     9721 cgtccgcggc gctgccgccg ttgccgcagg ccaggacctt gccgcctgcg cgcaggcggg
     9781 ccgcgatttc cgacgccagt tcctcgacgg gcccgcgcag gccgccgatc acctcctcga
     9841 cgaccgccag caggtcttcc gccatccggt cccagttcat gccggctccg ccgctcccgg
     9901 gcggcgcccc gcgccctcct cggccacgcc ccgggccgac gccacgatct cgcgcaccgt
     9961 gaccttgacg ccgcggatct cggtcaggcc gaggttctcg cggatgatct cgcggacgcg
    10021 gtcctgcatc atgcggcaga gctcggggat ttgcgtcccg ccgcgcactc ggacgtcgag
    10081 cgagacctcg atgctcccgc ccgccggccg cacggcgctg tccaggccca gcaccgcggc
    10141 gaactcgtcg cccacgcggg agacgacctc gccaatcgcg cgcgtgctga cgctgacgga
    10201 cccgctgtcg ttctcgaagg tgatgaacgg ctcgctgtac cggcgcggct tgaggccggc
    10261 caggaggtag acggccagcg cgcacaggac ggcggccgcc gcgcccagcg ccagccaccg
    10321 ctcgctccgg accaggtcca tcgcctgccg ccagtcctcg ggcgaacgcg cggccgcgat
    10381 cacggccgcg ccggccgcga cgaggaacag gaacaggagc acgccggaca aaatgcgcag
    10441 gggtttcatg gggcctttcc ttcctcgcgg gccgggttct ggccggcccc gaactgcagc
    10501 gactggacca cgacattcac cgatttgacg ttcttgcccg tcatctgttc caccgcctgg
    10561 cgcacctcgc tctggacctg ccaggccacc tgcgggatgc ggacgccgaa atgcacgacg
    10621 acgtacaggt cgatgaccac gcccgtctcc agcagcagga cccggatgcc cgtgtcgtcc
    10681 atcttcctgc caaagacctt cgccaggccc tgggcgaacg acgggctcaa cgcggccacg
    10741 cccggcacct tcagggccgc cagccgcgag atggtggcga tgacgtggtg atgtatcttg
    10801 atctcgccct gatccatgcc ctcctcggcg ttgtcatccg ggggcacggc cgccccggct
    10861 gcccggaggg ggtggcgcgg gtccatcgtt gcattattct ccgccggtgt cgcttcgctc
    10921 atggccaggt cccccacggc tcacggtgtg atcaaaaaca cgtcctgcat gaagctctcc
    10981 aggaaggccg tcgtgtattc gccccgcgcg aaacgcgtgt cgccgagcag cgcctggcac
    11041 agcgggaccg tcgtcgcggg gccctccatc acaaattcgc tcaacgcccg ggtcatcttg
    11101 gcgatggcct ccgcccggtt gcggccgcgg acgatgatct tcccgatcat cgagtcgtag
    11161 tgcgggttga tctcgtagcc cgtgtagacg tgcgagtcca cgcggacccc cggcccgccc
    11221 ggcatggaga cgaactggat ccggcccggc gacggcgtga agttcttgta cgggttctcg
    11281 gcgttgatcc gcacctcgat ggcgtgcccc gcgaaggcca gatccttctg gctgaaggac
    11341 agcttgttcc ccgccgccac gctgatctgc tccttgatca ggtccacgcc cgcgacctcc
    11401 tcggtcaccg ggtgctcgac ctggatgcgg gtgttcatct ccatgaagta gaactggctg
    11461 gcctccacgt cgaagaggaa ctcgatcgtc cccgcgttgc ggtacttgac cgcctcggcg
    11521 ccgcgcacgg cggcgcgcgt cagtttcttg cggacgtccg gcgatatcgc ggggctcggc
    11581 gactcctcga tcagcttctg gtgccggcgc tggaggctgc agtcgcgctc gcccaggtgc
    11641 accacgttgc cgtggtggtc gccgaacacc tgcacctcga tgtgccgcgc cttctcgacg
    11701 aacttctcga tgtagacctc ggggttgccg aacgcccgct cggcctccga cgcggcggtc
    11761 atgaagctct gcaccaggct cacgtcgttg cgcgcgatgc gcatcccctt gccgcccccg
    11821 ccggcgacgg ccttgatcag cacgggatag ccgatctgcc gggcggcctt cagcgcctcg
    11881 tcctggttcg cgatcacgcc ctcgctgccg ggcgtcaccg gcacgcccgc cttcttcatc
    11941 gtctcgcggg ccttggcctt gtcgcccatc atccggatgt tgtccgcgct cgggccgatg
    12001 aaggtgatct tgcagttctc gcagatctcc gcgaagtgcg cgttctcgga aagaaagccg
    12061 tacccgggat ggatcgcgtc gacgtccgcc acctcggccg cgctgatgat gttggcgatc
    12121 ttgaggtagc tttccttcgc cggcgccggc ccgatgcaca ccgactcgtc cgccatctgc
    12181 acgtgcagcg aggattcgtc ggcctcggag tgcacggcga cggtcttgat gcccatctcc
    12241 cggcacgcgc ggatgatgcg cagggcgatc tcgccgcggt tggctatcag tatgcgttca
    12301 aacatggcgg tgtcttctcc gcgtcctccg ggccgggcct catgccgggt cgatcaggat
    12361 cagcgtctgg ccgtactgca ccgccgaggc gttgtccacc aggatcttct tcaccacgcc
    12421 gcgggtctcg gccttgatct cgttcatcac cttcatcgcc tcgatgatgc agacgatgct
    12481 gtcgggctcc acctgctggc ccacggtgac gaagggctcc gcgtccggcg aggccgcgcg
    12541 gtagaaggtt ccgacgatgg gtgacttgac cggcgtccac ttcgattcct ccgggacttc
    12601 cgcgggggcg gccggcgccg gcgcgggggc cacgccggga gccgtggcgg cggccgacac
    12661 gaccacgggg ggcgaggcca cgggcagggc ggaaatcgcc acgcgcggct cgccgcccag
    12721 cgaccgcttg atggaaatcc gcaggccttt ttcctcgacc tcgaactcgg acaggtcgtt
    12781 gtccttcatc agggccacga tctgtttaat cgtcagaatg tccatgcgat cattcctccg
    12841 tccttgcccg ccaccccgct cgcctacgac gccgtaaacc ttaccagccc ggttgcgccc
    12901 tgtccagaaa tccgccgcgc cgtcagcctt ccagccagag atgctcgaac gccagctcgt
    12961 ccgggatgtt ccgctcgagt tggcgatgca gggtctccac ggcccggaag cgccggagcg
    13021 cgcgggcata gtcgaggccc gcggcctgcc ggcgcagggc gtccgcctcc tccgggaaat
    13081 gcaggtcgcc gggctccagg cccaggacga gcagcagcgc gtcgcgccgc cactccatca
    13141 ggcaacggac gatgcccgcc cgggcctcga gc
//
LOCUS       DAOOTK010000094        13113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3168_length_13113_cov_4.492801, whole genome shotgun sequence.
ACCESSION   DAOOTK010000094 DAOOTK010000000
VERSION     DAOOTK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_13113_cov_4.492801"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK634_10405"
     CDS             <1..574
                     /locus_tag="PK634_10405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289593.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOW98187.1"
                     /translation="GTDMGELQERITSTKKGSITSIQAVYVPADDYTDPAPAATFAHL
                     DATTELSRAISELGIYPAVDPLTSTSTLLLPHIVGAEHYRVARGVQETLQRYKELQDI
                     IAILGMDELSEEDRLTVSRARKIQRLMSQPFHVAEQFTGIPGVHVPLADTIRSFREVL
                     EGKHDRVPEQAFYMAGSIDAVIEKARKLKG"
     gene            579..884
                     /gene="atpC"
                     /locus_tag="PK634_10410"
     CDS             579..884
                     /gene="atpC"
                     /locus_tag="PK634_10410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOW98188.1"
                     /translation="MATFHLTILTPEQVVFEGDVESLTAPGVAGCLGILAHHTPLLAA
                     VRRGVLHVRREEGRKYFAVDESFLHVNGNAAVLLADAAVEVADEHAARQAHEAMVRG"
     gene            complement(906..1361)
                     /locus_tag="PK634_10415"
     CDS             complement(906..1361)
                     /locus_tag="PK634_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOW98189.1"
                     /translation="MNLRKVLTPSAINVALKSSAKDAVIGELMDLLMATGKIRDLDAR
                     QKAIQAVLDRERKMSTGMQNGVAIPHGKTDTVDTLVAAIGLKKEGVDFQALDGQPSRI
                     FVMTLSPDTRTGPHIQFLAEISRQLSDAAVRERILKAATPEEVLDILAP"
     gene            1530..3212
                     /locus_tag="PK634_10420"
     CDS             1530..3212
                     /locus_tag="PK634_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOW98190.1"
                     /translation="MTIKEWLARSVARRGDGVALRHKKGGRWQSLTYRELLTRAWGVA
                     ELLARRGLKPGDRVAIFRENAPDWPEMYFGITGLGLAAVPVDAKLQEQEVAHILRDSG
                     ARLLFADARSYPLLKEIETQLPGLQAALLVGGRDLGPPPSRRVRYEDYREALESAAAA
                     AAADGRAYDRHSPAEGDIASFIYTSGTTGRQKGAMLTHGNFAANVEAILHAIEIRADD
                     NFLLVLPLHHAFAFTANLLTPVAAGCEISFVESLKTVGENTREVSPTVLIGVPLLIEK
                     MYNRIWAGLRENKAAWALYRLGLRRLVRRGIAARLGGKLRLIVTGGAPCDPDVLVNFS
                     NLGFPILEGYGMTETAPVLTLNPPGGPRPGTVGRPLPGVEIRIQDPNPEGIGEITARG
                     PNVMKGYYNNPEATAAAFRDGWFLTGDLGRLDADGYVTITGRKKSLIVNREGKNIYPE
                     EVEHAVSKSPFVRECLALGFRDPEVKVGEQVGIIVVPNQEEIDAHARRGHRTLSEAEA
                     VELVRRDVKRVCAGIAEYKRPRRIQVRWEEFGKTSTGKVKRYLYALRAEDLA"
     gene            3460..5490
                     /locus_tag="PK634_10425"
     CDS             3460..5490
                     /locus_tag="PK634_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOW98191.1"
                     /translation="MAPSLIQDLAVILLISGLTTWLFHVLNQPKVVGYVLAGILIGPH
                     TPPFALIQDEATIRTLADIGIIFLMFSLGLDFNLRRLRRVGATAVLTAILDVSVMIWL
                     GFLLGRKLGWTPLESLFLGAILCDSSTTILAKILHEMGRSHDRFAGVAIGITVVEDVL
                     AVGMIAVLTGLALTGSVQTGLVAVRLWHLVLFLVLVTVAGLLTVPRLLDRIHRFRNDE
                     VLIVVLVGLCFGVSLLAVRLELSLALGAVLVGALASESHALPRISALVEPLRHVFSAV
                     FFVTIGLRLDPLAVWAHLPTLGAVVLLVMGGKLLTNATGCLLTGHPLDTSLLVGAGMA
                     QIGEFAFIISALALSLHAGREIVYQVGVSAAVLTTLLNPYLLRVMDRSAGRLARSPRG
                     RRWRAGIYFYSQWIAQVGQRGQNRAVRRVVRRSLLVVSVNLILIAAIFGVAGYVQTVT
                     AAAGERLLGRPGLYAALLWLAAAATSLPLYVVTARKLAALALMLAETALPATLTAGWV
                     RPVRTFITHALLLASGIGLVLLTFMLSSTLLPSREVFVLLVLLTLASTVWAHRQMARV
                     YARAQAAIQTLLSSDRKQQPPDRQPILPDLAYEMELVPLVLPPSRAEPGARTLRTLKL
                     RTRTGATIVAFERDGRRIINPDPDTEFQAGDRIFLLGLPDQVRSARELLDRG"
     gene            complement(5501..6436)
                     /gene="fmt"
                     /locus_tag="PK634_10430"
     CDS             complement(5501..6436)
                     /gene="fmt"
                     /locus_tag="PK634_10430"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOW98192.1"
                     /translation="MRILFMGSGSVACPALRELAARREDAVVAVVTQPDRPSGRRREL
                     TACPAKSLALSLGLPVREPEKVGDPSEVDALKALAPDLIVVADYGQFLRPALLALPAK
                     GAINIHPSLLPKYRGAAPIQWAVANGETEAGVTILHVTEKMDAGDILLQEKVPIRDEH
                     TAATLTPLLAELGATLLIRAIESIRRGEATRRPQDEALATFAPKLTKEDGRVQWTLPA
                     EAIRNRTRGFTPWPGSFAIRPDGAGGLLRLLAVRVEPGAGEPGLVLECGPDGPLVACG
                     ENALRLLEVLPEGRKAMSGAEYARGYRLAPGLRFT"
     gene            6639..7838
                     /locus_tag="PK634_10435"
     CDS             6639..7838
                     /locus_tag="PK634_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOW98193.1"
                     /translation="MPIAQKAREMIEAVHRYGLKVPRTIVFGKSRLNELGDLAFPLAA
                     GKKALILLGGGSARRAGLDQRIAALLGRHGVAAEILEGVHGEPTVDVVDALAAKARAL
                     EPGLIVSAGGGSVMDAGKAVAALAVNEGSVEEYLEGEEQTRALAAPPLPHIAIPTVAG
                     TGAEMTRNAVIGHPGKKVKRSMRADSMIPTVALIDSMLTLNVPHDVTASGGMDALTQL
                     IESCISNRRRPETTALAQEGLRLAREAIAVSVEAPGNEAARDKMSLVSMLGGVCLANA
                     GLAMAHGIAAALGALFGVPHGLACGILLPYTLRFNAPACSAELAHALSAFLNQGGMAP
                     RIIEDGIAAVEALADAIGIPPDLKEMALTEEDVQEIARRSIGTSMSGNPIPMNPDQVA
                     EFLRPLV"
     regulatory      7893..8007
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8069..9316
                     /gene="cytX"
                     /locus_tag="PK634_10440"
     CDS             8069..9316
                     /gene="cytX"
                     /locus_tag="PK634_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HOW98194.1"
                     /translation="MKTSTDRPLSGADFFLLWMGAAISLAEIWAGGLLAPLGLFAGLA
                     AIVLGHVIGNTPMALGGIIGSRHEVPAMVSTRDALGHRGSYLPAVLNVIQLTGWTAVM
                     LRICGQAADAVVPGDRGIGVRGWILVSGILTTAWALTGHGLRRWLQRGAVTLLTALSL
                     LMTGIVFRRYGLADLLAIRPDGTLPFGQGLDLVIAMPISWLPLVSDYSRFARDSRSCF
                     WGTWWGYLLAGSWMYAVGLCAALAARTANPDGMVLGLMAETGLIGPALLIIILSTITT
                     TFLDMYSTAVSVRSLAPKWNERAVILAGGLLGTLLAVVFPATEYESFLLFIGAMFCPL
                     FGVVLADYFILRRMQFGPPPAGIRPDAMLAWAAGFALYQLATRQAWALGASLPALLGA
                     AGLHLALRAARHPRPGPSPGGAS"
     gene            9313..10761
                     /gene="thiD"
                     /locus_tag="PK634_10445"
     CDS             9313..10761
                     /gene="thiD"
                     /locus_tag="PK634_10445"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOW98195.1"
                     /translation="MKPIVWTVAGTDPGGGAGIQGDLATLNGLGVHGGSVITAVIAQN
                     TLGVQRVEFAPADLVAAQLDSLREDLPPAAIKIGMLGTAGAARAVARVIRDLDAFVVY
                     DPVMGSSSGHGLMAPDLLPAVIEDVLPLVDLLTPNLPEAQRLTGRAARAAEDLPHVAG
                     ALLALGARTVLLKGGHSDNREYCQDFWTDGKVSWWITSPRQAVRHTHGTGCTFSSAAA
                     ACLARGYAIEDALVLAKAYVNQGLRLGGGIGRGRGPLAHEGWPNHPDDAPWMTAHAAA
                     GLSRPAFPPCEKPVGLYPIVDRAAWIGKLAPLGVNLVQLRAKDLSGEALDREAREAIR
                     LARAAGVRLVINDRADLALRHGAWGIHLGQDDLPGADLAAIEKAGLRLGVSASSYADM
                     ARALALRPSYIGLGAVYATATKAIGYAPLGVESFGRLRSWSSAPVVAIGGITVENARP
                     LRDAGADGVAVISDLRDAADLAARVRAWQAVI"
     gene            complement(10762..11466)
                     /locus_tag="PK634_10450"
     CDS             complement(10762..11466)
                     /locus_tag="PK634_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98196.1"
                     /translation="MNVLFALMMAGVMLFSGVSQFKAPRSGALAEGPGELVPAAEPVP
                     DAAVEPMAEASETPAEEAVAAPVEEEPGVPTFAVFLPERIERVWYWYAYTEETQHIVQ
                     SAVEKALINAGLAVVDVASMPLPEQGDLAQVMSAPQVVRWGAAARADYIIMGTATADP
                     QSESVAYGQKVVRSTANLSARIIRVSDSRIVAVQDASALMGGQALAAAAREALKKGGE
                     DIGRKLARSAKNLPKP"
     gene            11602..11677
                     /locus_tag="PK634_10455"
     tRNA            11602..11677
                     /locus_tag="PK634_10455"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11635..11637,aa:Ala,seq:ggc)
     gene            11789..12037
                     /locus_tag="PK634_10460"
     CDS             11789..12037
                     /locus_tag="PK634_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOW98197.1"
                     /translation="MDSFHYVYRLRSLRVPTQTYIGLTKNLRLRLLKHNEGGVPHTAK
                     FRPWTLDLAIAFPSRDKAAAFEAYLKTGSGREFARRHF"
     assembly_gap    12093..12192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12301..12855
                     /locus_tag="PK634_10465"
     CDS             12301..12855
                     /locus_tag="PK634_10465"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOW98198.1"
                     /translation="MKTPPAHTRFNQLPFRWPDPPFHIVLVNPEIPPNTGNIARLCAA
                     TGSILHLIEPLGFKITDSELKRAGLDYWKSVDIRVHTDFDAYLAEERPARLFLFSTAG
                     GRSFHDARFRPGDALVFGCETRGLPEHLLARYPGQVLGIPVRPEHVRSLNLSTAAGIA
                     LYEALRQAQPEWITTKDTKNAKGK"
BASE COUNT         1909 a         4645 c         4351 g         2108 t
ORIGIN      
        1 gggcacggac atgggcgaac tgcaggagcg gatcacgtcc acgaagaagg gctccatcac
       61 ctccatccag gccgtgtacg tgcccgcgga cgactatacc gacccggccc cggcggcgac
      121 gttcgcccac ctcgacgcga cgacggaact ctcgcgcgcc atttcggagc tgggcatcta
      181 ccccgcggtg gacccgctca cctccacctc cacgctgctg ctgccccaca tcgtcggcgc
      241 cgagcactac cgggtggcgc ggggcgtgca ggagaccctc cagcggtaca aggaactgca
      301 ggacatcatc gccatcctgg ggatggacga actgtccgag gaggaccggc tgaccgtttc
      361 gcgcgcccgg aagatccagc gcctgatgtc gcagccgttc cacgtggcgg agcagttcac
      421 ggggatcccc ggcgtccacg tcccgctcgc ggacaccatc cggtccttcc gggaagtgct
      481 ggaaggcaag cacgaccggg tccccgagca ggcgttttac atggcgggct cgatcgacgc
      541 cgtgatcgag aaggcgcgca agctgaaggg ctaggcccat ggccacgttt catctcacga
      601 ttctgacgcc ggagcaggtg gttttcgagg gggacgtgga gagcctgacc gcgcccggcg
      661 tggccggctg tctcgggatc ctcgcccatc atacgccgct gctggccgcc gtacggcgcg
      721 gggtgctcca cgtgcgccgg gaagagggcc gtaagtactt cgccgtcgac gagagtttcc
      781 tgcacgtcaa cgggaacgcc gcggtgctcc tggcggacgc cgccgtggaa gtcgcggacg
      841 aacacgcggc ccggcaggcc cacgaggcga tggtccgggg ttgaagccgc gggaagcggc
      901 gcctcctacg gcgccaggat atccagcact tcctccggcg tggcggcttt caggatccgc
      961 tcgcggacgg cggcgtcgct caactgccgg ctgatctcgg cgaggaactg gatgtgcggg
     1021 cccgtccgcg tgtccggcga gagggtcatc acgaagatcc gcgaaggctg tccgtccagg
     1081 gcctggaaat cgacgccttc cttcttgagc ccgatggcgg cgaccagcgt gtccaccgta
     1141 tccgtcttgc cgtgggggat ggcgacgccg ttctgcatgc cggtggacat cttccgctcg
     1201 cggtccagga ccgcctggat ggccttctgc cgggcgtcca agtcgcggat cttaccggtc
     1261 gccatcagca ggtccatcag ttcgccgatg acggcgtcct tcgcgctgct tttcagcgcc
     1321 acgttgatcg ccgacggggt caggaccttt cgcaggttca tggtttcttc ctggcaaaat
     1381 aggccggtat aatgtcgcca tccggccgcc caggtcaata ccagagtggg gccctcatcc
     1441 accgcccgtc cgcgccgaaa aaagcggctt ccgcgggccg ggccggcctg ctatcttcac
     1501 aaccggcgga gcggatctgg agaacccgca tgacgatcaa ggaatggctg gcccgttcgg
     1561 tggcccggcg cggcgacggg gtcgcgctgc ggcacaagaa gggcggccgc tggcaaagcc
     1621 tgacctaccg cgaactgctg acgcgggcgt ggggcgtcgc cgaactcctg gcgcggcgcg
     1681 gcctgaaacc cggcgaccgg gtggcgatct tccgcgaaaa cgcgccggac tggccggaga
     1741 tgtacttcgg gatcaccggc ctcggcctgg ccgccgtgcc cgtcgacgcc aagctccagg
     1801 aacaggaagt ggcccacatc ctccgcgact ccggcgcgcg gctcctcttc gccgacgccc
     1861 gcagctatcc cctcctgaag gaaatcgaga cgcaactgcc cggcctccag gccgcgctgc
     1921 tggtcggcgg ccgcgacctg ggccccccgc cctcgcggcg ggttcgttac gaggactacc
     1981 gcgaggccct cgaatcggcc gccgcggcgg cggccgcgga cggccgcgcc tacgatcgcc
     2041 actcgccggc cgagggggac atcgcctcgt tcatctatac ctccggcacc acgggccggc
     2101 agaagggcgc gatgctgacg cacggcaact tcgccgccaa cgtggaagcc atcctgcatg
     2161 cgatcgagat ccgcgcggac gacaacttcc tgctcgtgct gccgctccac cacgccttcg
     2221 ccttcacggc caacctcctg acgcccgtgg ccgcgggctg cgaaatcagc ttcgtggaga
     2281 gcctgaagac cgtcggcgag aacacgcgcg aggtctctcc caccgtcctg atcggcgtgc
     2341 cgctgctgat cgagaagatg tacaaccgga tctgggccgg gctgcgggag aacaaggcgg
     2401 cctgggcgct ctaccggctg ggcctgcgcc ggctcgtgcg ccgcggcatc gcggccaggc
     2461 tcggcggcaa gctgcgcctc atcgtcaccg gcggcgcccc gtgcgatccg gacgtgctcg
     2521 tgaatttctc gaatctcggc ttcccgatcc tggagggata cgggatgacc gagacggcgc
     2581 cggtgctcac cctcaacccg ccgggcggac cccggcccgg cacggtgggg cgtccgctgc
     2641 ccggcgtcga gatccgcatc caggatccca atcccgaggg catcggcgag atcacggccc
     2701 gcggcccgaa cgtcatgaag ggctattaca acaaccccga ggccaccgcg gccgcattcc
     2761 gggacggctg gttcctcacc ggcgacctcg gccgcctgga cgcggacggc tacgtgacca
     2821 tcaccggccg caagaaaagc ctcatcgtca accgcgaggg caagaacatc tatccggagg
     2881 aggtcgagca cgccgtcagc aagagcccct tcgtgcggga atgcctggcg ctgggtttcc
     2941 gggacccgga ggtcaaggtc ggcgagcagg tcggcatcat cgtcgtgccg aaccaggagg
     3001 agatcgacgc ccacgcccgg cgcgggcacc ggacgctgtc cgaggccgag gccgtggaac
     3061 tcgtccgccg ggacgtgaag cgggtgtgcg ccgggatcgc cgagtacaag cgcccgcgcc
     3121 ggatccaggt ccgctgggag gaattcggca agacctcgac gggcaaggtg aagcgctacc
     3181 tgtacgccct ccgggccgaa gacctggcct gatccggccg gcgggccgac tggatttccc
     3241 cgcgtttggt tgtattcgtt tagcatgacc ggacacggca agccgacgaa ccgctgttca
     3301 agcggccgcg aaatccgcag gacacccccc tgcccagcgg aggcgcgatg gcatgccgcg
     3361 ttctgctgaa gctgtcccgg ctaggcccgc gcgggtttgc gctggccatc ccgcgccccg
     3421 gccgctaggg tgagcaaagg acaagcgcgg cggaacatca tggcaccgtc tttgatacag
     3481 gacctggcgg tcatcctcct gatctcggga ttgaccacct ggctgttcca cgtcctgaac
     3541 cagccgaagg tcgtcggcta cgtgctcgcg ggcatcctca tcgggccgca cacgcccccg
     3601 ttcgccctga tccaggacga ggccaccatc cgcaccctgg cggacatcgg gatcatcttc
     3661 ctcatgttct cgctgggcct ggatttcaac ctgcggcggc tccgccgggt cggggccacc
     3721 gccgtcctga ccgcgatcct ggatgtcagc gtgatgatct ggctggggtt cctcctcggc
     3781 cggaagctgg gctggacccc gctggagagc ctgtttctcg gggctatcct ctgcgattcc
     3841 tccaccacga tcctggcgaa gatcctgcat gaaatgggcc ggtcccacga ccggttcgcc
     3901 ggcgtggcca tcggcatcac ggtggtggag gacgtgctgg ccgtaggcat gatcgcggtg
     3961 ctgaccggcc tcgcgctgac gggctccgtg cagaccggct tggtcgccgt ccgcctgtgg
     4021 cacctcgtcc tcttcctcgt cctcgtgacg gtcgcggggc tgctgaccgt gccgcgcctg
     4081 ctggaccgca tccaccgttt ccgcaacgac gaggtgctga tcgtcgtgct cgtcggcctg
     4141 tgcttcggcg tatccctgct ggcggtgcgc ctggagttga gcctcgcgct cggcgccgtc
     4201 ctggtcggcg ccctcgcctc ggaatcgcac gcgctcccgc gcatctcggc gctcgtggag
     4261 ccgctgcgcc acgtcttcag cgcggtcttc ttcgtcacga tcgggctgcg gctggacccg
     4321 ctcgccgtgt gggcccacct cccgacgctc ggggccgtcg tgctgctggt gatgggcggg
     4381 aaactgctga ccaacgcgac cggctgcctg ctgaccggcc atcccctgga cacctcgctg
     4441 ctcgtcggcg ccggcatggc gcagatcggc gagttcgcgt tcatcatttc cgccctcgcc
     4501 ctctcgctgc acgcgggccg ggagatcgtc taccaggtgg gcgtcagcgc cgccgtcctg
     4561 accacgctcc tgaatcccta cctcctgcgc gtcatggatc gctcggccgg gcgcctggcg
     4621 cgcagcccgc gcggccggcg ctggagggcg ggcatctact tctacagcca atggatcgcg
     4681 caggtgggcc agcgcggcca gaaccgcgcc gtgcggcgcg tggtccgccg ctccctgctc
     4741 gtcgtgtcgg tcaacctgat cctgatcgcc gccatcttcg gcgtcgccgg ctatgtgcag
     4801 acggtcaccg ccgcggccgg cgaacggctc ctgggccggc ccggcttgta cgcggccctg
     4861 ctctggctgg ccgccgccgc caccagcctc cccctctacg tcgtgaccgc gcgcaagctc
     4921 gcggccctgg ccctgatgtt ggccgaaacc gcgctgcccg ccacgctcac cgccggctgg
     4981 gtgcgcccgg tgcgaacctt catcacgcac gccctcctcc tcgcgagcgg catcggcctg
     5041 gtgctgctga cgttcatgtt gagttccacc ctcctgccgt cgcgggaagt cttcgtcctg
     5101 ctcgtgctgc tgaccctggc cagcaccgtg tgggcgcacc ggcagatggc ccgggtgtac
     5161 gcccgcgccc aggccgcgat ccagaccctg ctgtcgagcg accggaagca acagccgccc
     5221 gaccggcagc ccatccttcc cgacctggcc tatgaaatgg aactcgtgcc cctggtcctt
     5281 ccgccgtccc gcgcggagcc cggggcgcgc accctgcgga cgctgaagct gcgcacgcgg
     5341 acgggggcaa ccattgtagc cttcgagcgg gacggccggc gcatcatcaa tcccgacccg
     5401 gacaccgagt tccaggcggg cgaccggatt ttcctgctcg gcctgccgga ccaggttcga
     5461 tcggcgcggg aactcctgga ccggggatga gactccgcgg tcaggtgaat cgcaggccgg
     5521 gcgccaggcg gtagccgcgc gcgtattccg cgccggacat ggctttccgg ccctccggca
     5581 ggacctcgag gaggcgcagc gcgttttcgc cgcacgcgac gaggggaccg tcggggccgc
     5641 actccaggac aaggccgggt tcgcccgctc ccggttcgac gcggaccgcg agcaggcgca
     5701 ggagcccgcc ggccccgtcc ggacggatcg cgaagctgcc cggccagggt gtaaagccgc
     5761 gggtccggtt gcggatggcc tccgcgggaa gggtccattg aacccgcccg tcctccttcg
     5821 tcaacttggg cgcgaaggtg gccagggcct cgtcctgcgg ccggcgcgtc gcctcgccgc
     5881 ggcggatcga ctcgatcgcg cggatcagca gcgtggcgcc cagctcggcg agcagcgggg
     5941 tcagcgtcgc ggccgtgtgc tcgtcgcgga tcgggacctt ctcctggaga aggatgtcgc
     6001 cggcgtccat tttttccgtg acgtgaagga tggtcacccc ggcttccgtc tcgccgttgg
     6061 cgacggccca ctggatcggc gccgcgccgc ggtacttcgg gaggagggac ggatgaatat
     6121 tgatggcgcc cttcgcgggc agcgccagga gggcgggccg caggaactgg ccgtagtccg
     6181 ccacgacgat caggtccggg gccagggcct tcagggcgtc gacctccgag ggatcgccca
     6241 ccttttccgg ctcgcgaacc gggaggccca gcgacagggc cagggacttg gccgggcagg
     6301 ccgtcaactc ccggcggcgt ccgctgggcc ggtcgggctg ggtgaccacg gccacgacgg
     6361 cgtcctcccg ccgcgcggcg agttcccgca gcgccgggca ggccaccgat cccgagccca
     6421 tgaagaggat gcgcatgaca gcgcctcctt tctcacaccg cgccggtccg cgcaacgaca
     6481 aattcctccg ccttccccgc cggcggccgg taacaatata cttcacacgt gtgaggtagt
     6541 ttgttacgca cggaattccg ccggggccgg ccgcgctgga cagaaaggcg cgatcccgct
     6601 atcctgtatc cagcaacacc gggggattca tgccgaccgt gcccatagcg cagaaggcgc
     6661 gcgaaatgat cgaggccgtc caccggtacg gactcaaggt tccgcgcacg atcgtgttcg
     6721 gtaagagccg cctgaacgaa ctgggcgacc tggccttccc cctggccgcc ggcaagaagg
     6781 cgctgatcct gctcggcggc gggtcggcgc ggcgggccgg gctggatcag cgcatcgccg
     6841 cgctgctcgg gcggcacggc gtcgcggcgg aaatcctgga aggcgttcat ggcgagccga
     6901 ccgtggacgt cgtggacgcc ctggccgcga aggcgcgcgc cctggaaccc ggcctgatcg
     6961 tcagcgcggg cggcggcagc gtgatggacg ccggcaaggc ggtggccgcc ctcgccgtca
     7021 acgagggctc ggtggaggag tacctggagg gcgaggaaca gacccgcgcc ctggcggcgc
     7081 ccccgcttcc ccacatcgcc attccgaccg tcgcgggcac cggcgcggag atgacccgca
     7141 atgccgtgat cggccacccg gggaagaaag tgaaacgaag catgcgcgcc gattcgatga
     7201 tccccacggt ggcgctgatc gactccatgc tcacgctgaa cgtcccccac gacgtcacgg
     7261 cctcgggcgg catggacgcg ctgacccagt tgatcgaatc ctgcatcagc aaccggcgtc
     7321 gccccgagac gacggcgctg gcccaggaag gcctgcgcct ggcccgcgag gccatcgccg
     7381 tcagcgtgga agcgccgggc aacgaggctg cgcgcgacaa gatgtcgctg gtcagcatgc
     7441 tcggcggcgt ctgcctcgcg aacgccgggc tggccatggc gcatggcatc gcggcggcgc
     7501 tcggcgcgct cttcggcgtg ccgcacgggt tggcctgcgg catacttctc ccctacacgc
     7561 tccgcttcaa cgcgcccgcc tgttccgccg aacttgccca tgcgctttcc gcgttcctca
     7621 accagggcgg catggccccg cgcatcatcg aggacgggat cgccgcggtg gaggcgctcg
     7681 ccgacgccat cggcataccg cccgacctca aggaaatggc gttgacggag gaggacgtgc
     7741 aggaaatcgc ccgccggtcc ataggcacca gcatgtccgg caacccgatc cccatgaatc
     7801 ccgaccaggt ggccgagttc ctgcgcccgc tggtgtgacg gcccggctcc ttttcgcgtg
     7861 ttgacagacc ttccgccccg tacgactatt ggcctgttcg ctgggggtgc cccactgcgt
     7921 gtcggcgggg ctgagagttc ccggttcggg ataacccctt gaacctgttc gggtcatgcc
     7981 gacggaggga agcgatgaga ccggctcctg aatcctcgcg gagcgttttt cattcccccc
     8041 ggaacggtga atcgtgcgga gggaatgcgt gaaaacgtcg actgatcggc cactttccgg
     8101 ggcggatttt ttcctgctgt ggatgggcgc cgccatttcg ctcgccgaaa tctgggcggg
     8161 cggcctgctg gcgcccctgg gcctcttcgc cggcctcgcg gcgatcgtgc tgggccacgt
     8221 gatcgggaac acgcccatgg ccctcggtgg catcatcggc agccggcacg aagtgcccgc
     8281 catggtcagc acccgcgacg ccctgggcca ccgcggctcc tatctgcccg cggtgttgaa
     8341 cgtcatccaa ctgacgggct ggacggcagt catgctgcgg atctgcgggc aggcggcgga
     8401 cgccgtggtt cccggtgatc gcggcatcgg ggtccggggc tggatcctcg tctcgggaat
     8461 cctgaccacg gcgtgggcgc tgaccgggca cggcctccgg cgctggctcc agcgcggcgc
     8521 ggtgaccctg ctgaccgcgc tctcgctcct gatgacgggc atcgtgttcc gtcgatacgg
     8581 cttggccgac ctgctcgcga ttcgcccgga cggcacgctg ccgttcggcc aggggctcga
     8641 cctcgtgatc gccatgccga tctcgtggct ccccctggtg tcggattact cgcgctttgc
     8701 ccgggactcg cgcagttgct tctggggcac gtggtgggga tacctgctgg caggaagctg
     8761 gatgtacgcc gtggggctct gcgcggcgct ggccgcccgg accgccaacc ccgacgggat
     8821 ggtgttgggg ctgatggccg agaccgggct gatcggcccc gccctcctga tcatcatcct
     8881 ctctacgatc acgacgacgt tcctcgacat gtactcgacc gccgtgtccg tccgcagcct
     8941 ggcgccgaaa tggaacgagc gggcggtcat cctggccggc ggactgctgg gcacgctgct
     9001 ggccgttgtt ttccccgcga cggaatacga atcgttcctg cttttcatcg gcgcgatgtt
     9061 ctgccccctg ttcggcgtgg tgctggcgga ctatttcatc ctccgccgca tgcaattcgg
     9121 ccccccgcct gccggaatcc gtccggacgc catgctggcc tgggcggccg gattcgccct
     9181 ctaccaactg gccacccgcc aggcctgggc cctcggcgca tcgctgccgg ccctgctcgg
     9241 cgccgccggt ctccacctgg cgctccgggc cgcgcgccat ccgcggcccg gcccctcccc
     9301 gggaggcgct tcatgaagcc catcgtctgg accgtggcgg gaaccgatcc cggcggcggg
     9361 gccgggatcc agggcgacct cgcgacgctg aacggcctcg gcgtccacgg cggctcggtc
     9421 atcacggccg tgatcgcgca gaatacgctc ggcgtgcagc gcgtggagtt cgcgccggcc
     9481 gatctcgtgg cggcccagtt ggattccctg cgcgaggatc tgccgcccgc cgccatcaag
     9541 atcggcatgc tgggcaccgc cggcgccgcc cgcgcggtcg cccgggtgat ccgcgacctg
     9601 gacgccttcg tcgtgtacga tcccgtcatg ggctccagca gcgggcacgg gctcatggcg
     9661 cccgacctgc tccccgccgt gatcgaggac gtgctgccgc ttgtggatct cctgacgccg
     9721 aatcttcccg aggcgcagcg gctcacgggc cgcgcggccc gggccgcgga ggatctgccg
     9781 cacgtcgctg gcgccctgct cgcgctgggc gcccgcacgg tgctgctcaa gggaggccac
     9841 tccgacaacc gcgaatactg ccaggatttc tggaccgacg ggaaagtctc gtggtggatc
     9901 accagcccgc gccaggcagt ccggcacacg catggaaccg gctgcacctt ctcgtccgcg
     9961 gccgccgcct gcctcgcgcg gggctacgcg atcgaggacg cgctggtcct ggccaaggcc
    10021 tacgtgaacc agggcctccg gctcggcggc ggcatcgggc gcggccgcgg ccccctcgcg
    10081 cacgagggat ggccgaatca cccggacgat gcgccctgga tgaccgccca tgcggcggcg
    10141 ggcctctccc ggccggcatt tcccccttgc gaaaaacccg tgggcctcta tcccatcgtg
    10201 gaccgcgccg catggatcgg gaaactcgcc ccgctcggtg tgaacctggt ccaactgcgc
    10261 gccaaggatc tatccggcga agccctggac cgcgaggcgc gcgaagccat ccgcctggcg
    10321 cgcgcggccg gggtccggct ggtgatcaac gaccgcgcgg acctggcgct ccgccacggg
    10381 gcgtggggca ttcacctggg gcaggacgat ttgcccggag ctgatctcgc ggccatcgaa
    10441 aaagcaggcc tgcgcctggg cgtcagcgcg agttcgtacg cggacatggc ccgggccctc
    10501 gccctgcgcc cgtcctacat cggcctgggg gccgtgtacg ccacggcgac caaggcgatc
    10561 ggttacgcgc cgctgggagt cgagtccttc ggccgattgc gctcgtggag cagcgcgccc
    10621 gtcgtggcca tcgggggcat cacggtcgag aacgcgcgcc cgctccggga cgccggggcg
    10681 gacggggttg ccgtgatttc ggacctgcgg gatgccgccg atctcgcggc gcgcgtccgg
    10741 gcctggcagg ccgtgatcta gtcagggctt ggggagattc ttggcggacc gggccagctt
    10801 ccggccgata tcttcgccgc ccttcttgag ggcctcgcgg gccgccgcgg caagggcctg
    10861 cccgcccatc agggccgagg cgtcctgcac cgccacgatc cgcgaatcgc tcacccggat
    10921 gatccgggcg gagaggttgg ccgtcgagcg gaccaccttc tggccgtagg ccacgctctc
    10981 gctctgcggg tcggccgtgg cggttcccat gatgatgtaa tcggcgcggg cggcggcgcc
    11041 ccagcgcacc acctgcgggg cggacatcac ctgggccagg tcgccctgct cggggagagg
    11101 catgctggcc acgtccacga ccgccagccc cgcgttgatc agggccttct ccacggcgct
    11161 ctgcacgatg tgctgggttt cctcggtata cgcgtaccag taccacaccc tctcgatccg
    11221 ctcgggcagg aaaacggcaa acgtcgggac acccggctcc tcttcgaccg gggcggccac
    11281 ggcctcctcg gccggcgttt cggatgcttc cgccatgggc tcgacggccg cgtccgggac
    11341 gggttccgcc gcggggacca actcaccggg cccctccgcc agggcgccgg aacggggagc
    11401 cttgaactgg ctcactcctg aaaacagcat gaccccggcc atcatcagcg cgaatagaac
    11461 gttcatgaag gttctccctc gtcgccaact cgcttgccga acaggctgaa aaagtacatc
    11521 ggggacattg acaaatcaag gccggcttcc tattgtcccc tagcctcgat gccggaatcc
    11581 ctttcggcgt cgcgcccggt tggggggtta gctcagttgg gagagcgcct gaatggcatt
    11641 caggaggtcg ggagttcaaa tctcctaccc tccaccagcc ttcgcgcggc cggccaaggg
    11701 ccgcgcgaag gctgtccgcc ggagtccccg ccacgggcgg ggcgaaggcg gacgggcaga
    11761 cagcggccgc ggctccggct ggcaagccat ggatagcttt cactacgtct accggctccg
    11821 cagcctacgg gtccccaccc aaacctacat cggcctcacc aagaacctgc gcctccgact
    11881 tctcaagcac aatgagggcg gcgtgcccca caccgccaag ttccggccct ggaccctcga
    11941 cctcgccatc gccttcccct cccgcgacaa ggccgccgct ttcgaggcct atctcaagac
    12001 cggctccggc cgcgaattcg cccgcagaca tttctaattc gcgcggccgg ccaagggccg
    12061 cgcgaaggct gtccgccgga gtccccgcca cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nntaattcgc gcggccggcc aagggccgcg cgaaggctgt ccgccggagt
    12241 ccccgcccct tctcgcgtgt atcatccacg agcctctttt gctatgaatt gagcctgacc
    12301 atgaagacgc cccctgctca cacacgcttt aaccagctgc cgttccgctg gccggatccg
    12361 ccctttcaca ttgtcctggt gaaccccgag attcccccga acactgggaa catcgcgcgg
    12421 ctgtgcgcgg ccaccggctc gatcctacac ctgatcgagc cgctcgggtt caagatcacg
    12481 gactccgagc tcaagcgggc cggactcgac tactggaagt cggtggacat ccgcgtgcac
    12541 acggatttcg acgcctacct cgccgaggag cgcccggccc gcctcttcct gttcagcacc
    12601 gccgggggga ggtccttcca cgacgccagg ttccggcccg gggatgccct ggtcttcgga
    12661 tgcgagaccc gcggcctgcc ggagcatctc cttgcccgct accccgggca ggtcctcggc
    12721 atccccgtgc gccctgagca cgtgcgctcg ctcaaccttt ccacggccgc cgggatcgcc
    12781 ctctacgagg ccctgcgcca ggctcagccg gaatggataa ccacgaaaga cacaaaaaac
    12841 gcgaaaggga agtagcccgg ttcttttttt cgtgtatttc gcgtgtttcg tggttctctt
    12901 tttcttgaac tttttgcctc gcggacggtc ttactatatc gtgcgtttcg catagggaag
    12961 gagaactcta tctatgaata cgggtatcgg catcatcaac gagcgggtga agcaggagag
    13021 ccagtttatc gcgggtctcc gcagcgagat cgagaaggtc atcatcggcc agaaatacct
    13081 ggtggaccgc ctgatcgtcg gcctgctggg caa
//
LOCUS       DAOOTK010000095        13103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3173_length_13103_cov_5.129139, whole genome shotgun sequence.
ACCESSION   DAOOTK010000095 DAOOTK010000000
VERSION     DAOOTK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_13103_cov_5.129139"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK634_10470"
     CDS             complement(<1..363)
                     /locus_tag="PK634_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98199.1"
                     /translation="MADPADLKAIEFFAGCTEQELRDLAPFLLSNLLLDRLKASVPAR
                     IVNVNAGLYVKGEADPEKLPTGENFNRFKTYMHSKLCNMYFTTELARRLEGSGVTVTA
                     LHPGVTVELVAIRTQGDIL"
     gene            complement(341..1042)
                     /locus_tag="PK634_10475"
     CDS             complement(341..1042)
                     /locus_tag="PK634_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HOW98200.1"
                     /translation="MQPPLTSDEVRILGALIEKEITTPEYYPLSLNALTNACNQKSNR
                     FPVVALDDRMTDSLLEGLRDKRLVAMVTGTSNRVPKFRQLLVETIGLDSKDMAVMGEL
                     MLRGPQTAAQLRAHAARLHPFADQAEVEAVLQGLSERPQGPLVVKLPRQTGHKEQRFA
                     HLLGGAPAAPEAVAAPPPEPASIEGTPEADPLAQLRAEVESLRAEVADLRRQVAELKG
                     PLPAEGAPDGGPGGS"
     gene            complement(1089..1472)
                     /locus_tag="PK634_10480"
     CDS             complement(1089..1472)
                     /locus_tag="PK634_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOW98201.1"
                     /translation="MNAWYASLTLPPLTPPSWVFGPAWTILYTLIAVSIVVYYRARPL
                     QGRLVTTVVLAFHLLTNFAWSPLFFGLQRPGLALADIVLLDVSLVAVILLFRRASRLA
                     AALLAPYLLWVLFATYLNAGIVALN"
     gene            complement(1517..4243)
                     /locus_tag="PK634_10485"
     CDS             complement(1517..4243)
                     /locus_tag="PK634_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOW98202.1"
                     /translation="MSGFPGRSAAGYLLFLLAAGEATALTVSNVQYTTAAGGASPYSG
                     SSVTVSGVAVYADPQGYTLSDAGGGPWSGVYINDPFHRPNPGDRVTLGGTVRESRNMT
                     VLTNVSSYSLTSTGAPVAATWMPGRQVTNEAYEGVLVRVTNATVRNINVRGTETYWQA
                     GDTSANFYVATRVPYRYVWVSNQVLSAIQGVVFSVGTTNSISPRSDDDLVGRTVFEYA
                     LRGLVMTPGGPRTNWYVHVRDDDIVAVTSAAPPGVTAVDTGGIIFPGLLDVHNHPAYN
                     SFPTLMFNNFPFGHRDQWGEDDAEYDDWKNRRIALRTAEGDSTNDTITKYGECLELMA
                     GCVAIQGQSNNDPEHTHPDVILYNIEEFPSRVWCNIFPWRMTNTSGIAERTNLLLRIA
                     GGAVNAAMIHLSEGTDTTARAQFATWRGWGMLNSSVAIIHGAALGTNEFAQMAAAGAK
                     LIWSPMSNMKLYAGTANIRAAKQAGVVIGLSPDWTPSGCFNLLEELGYAWELNQSVFS
                     NAFTARELCDMVTINNARCAGLTNRYGQIAAGFNAGLAVIEGDPADPYLSLIRARPSS
                     VLLTVVDGTPRYGDPALMSSLGVSGESVDVRGRTKIFNIAVSHPFLEHSQRRFTNLVA
                     MLRTAHATLAPVNELDREELQLLDLAFLQRSGDDVVPFRADNPISAGPGGGTYDLGSN
                     LSLTFRYQDFWDNDAFITGLTHTISIVPARYSNLVLQTIATNWPNDPARETVNFTIDF
                     QDMHTNYIFSFVTRDLQGNGRTSQIGAVSFRLAAHAGGDSDRDGLPNEWETLYFGFFT
                     NATAGANSDGDLLDNLEEYVADTVPTNAASCFSSYVQAIAEAGGVMQLRSPVPTSAQR
                     RYEAWWTTNLAGDATWTPAGLNLAGAADGSAVTLSVTNPLPAAFYRVGVKLP"
     gene            complement(4327..5145)
                     /locus_tag="PK634_10490"
     CDS             complement(4327..5145)
                     /locus_tag="PK634_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOW98203.1"
                     /translation="MKKIGLALGGGGAKGLAHVPMLEILDELGVRPYRIAGTSIGAVV
                     GALYASGLSGARIREGVRKMVVSRRDTLREALRKTDALKWFGFLDLEFGRRGLLKGDK
                     FIEFLYGKMGVRTFEELKIPLRVVATDFNTAEQVLLDSGSLPDAIKASMGLPGVFTPV
                     ARAGRVLIDGGGVNPVPWDVLDDCDFVVAVDVMGDLDPAGPPVPNLFRAVLGTFDIMQ
                     KSIIAEKLRRAPPDLYIRPAVRGVDILDFRRAEDVFEMAAPAAQELKQALQRVL"
     gene            5248..6333
                     /locus_tag="PK634_10495"
     CDS             5248..6333
                     /locus_tag="PK634_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOW98204.1"
                     /translation="MLYRPFGQTGKRVSVIGAGCMRFETPQNDRDIGRAAETLLHAHA
                     RGINYFDTAPYYCGDRSEDIVGAAVRQLKPGTFYLSSKCDKADGAAFRKGLERSLQRL
                     GVDRIHFFHIWCLMNPTQWAERKDRGAVREALRAKEEGLIEHLVFSSHMDGEETAAVI
                     AEGIFEGVTLGFNAANFRFRRSALEAAARAGLGVVTMNPLAGGLIPAHPKRFDFIREK
                     NDPDVVTAALRFNLSHAAITCALVGFRNPADVDSALAALDEFAPRTDAEIARVERHLE
                     EGFDGLCTGCEYCLPCPADIPIPRMMDSYNMLILEGEKKAVQERLHWQWSLDQTVAGQ
                     CTECGACEERCTQHLPIIRRLKEIRAL"
     gene            6416..7681
                     /locus_tag="PK634_10500"
     CDS             6416..7681
                     /locus_tag="PK634_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98205.1"
                     /translation="MSKRMAFLAAGLLLGAATVPAQAQQSLQALVNSATGSVIEVPAG
                     EYQGPVRLKEGVLLVGAGADRTILNVPDAAPGVMGENQSGIIGFTIRGGQHGVRTLGR
                     FMGVFECRLEGQAQAAVLFEGGSGVAVNNAIVGSQTNQGVMILRSNPLVSRNVIAYHD
                     VGVHVSGEMIPPVQDNLFIGNRTAVLVDPGSKALLARNLYDRNGVAVQGADLGADDRI
                     EPIAWDGKVPAAGATTAQYRDLMNVVFEEAINAHPVVVYGLGEEMGAFDVVILSPSAT
                     FSIGASAVDTIISSHHAYDSATQDALASTLRVAERPFVDVVNPLVKDLAVDRYVLENR
                     YRHPASYGVNEKGQRVFRRMTNMNRAEIFCPAGWLPVGVNYPASFDRVGNQVVIKITH
                     PGVTNLEIAMEPDAASADPLGVRAALAGE"
     gene            7678..8094
                     /locus_tag="PK634_10505"
     CDS             7678..8094
                     /locus_tag="PK634_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOW98206.1"
                     /translation="MKTHQEEIRLETAGHRDTHDLTDAVAAVVGRSGVTTGLAHIFNV
                     GSTACVGMLEFEPGLQRDIGEVLDRLIPPSRAYGHEQAWHDGNGHSHLQATLMGPSLT
                     VPVSGGRLRLGTWQQIFHLECDVEPRSRVVVVTVTG"
     gene            complement(8078..9967)
                     /gene="recQ"
                     /locus_tag="PK634_10510"
     CDS             complement(8078..9967)
                     /gene="recQ"
                     /locus_tag="PK634_10510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOW98207.1"
                     /translation="MPATSQPSLSPEALAQVNMALRRYWGYESLKSLQAEAIACAMAQ
                     CDSLTVLPTGGGKSLCYQVPPLLDGSLDVVVSPLISLMKDQVDGLRESGYPAAALHSG
                     MTAAERRIVETALRAAEIRLLFVSPERLMMEDFLGDLKQLAVRRFAIDEAHCISQWGH
                     DFRPEYRQLAVLRQHFPSASLHAYTATATPRVREDIVEQLQLKDAALLVGCFDRANLI
                     YRVLPKTDVREQTAQAIARHAGEAVIAYCLSRKDTETLAAWLKARGVRAAAYHAGMEP
                     EDRHRTQEAFADESLDVVVATVAFGMGIDRSNVRCVIHTSLPKTIEHYQQETGRAGRD
                     GLEAECVLFYSAADVMRWKGIMNKPPAEAPEPGTEADLEAARKIQLELLRGMQRYCTS
                     ARCRHKALSEYFGQAYDRADCGACDVCLDEVEEVGDGTVLAQKILSCVARVGQRFGVG
                     HVVNVLLGADTETLRRAGHDGLSTYGLLKDSTASALTALVYQLVDQGLLTRTEGDRPV
                     LKLNEASAAVLRGGRAVRLFQPKERLRKRTARGEKSMEGVDMELFDTLRRWRQEECQR
                     LGIPAYVIFHDTTLAELARARPGRVEDLVGIPGIGEHKQARWGEAVCAIIAACVPPGR
                     VSPSR"
     gene            10625..11362
                     /locus_tag="PK634_10515"
     CDS             10625..11362
                     /locus_tag="PK634_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98208.1"
                     /translation="MNGRQVEVSNKPGRESIMRLRFGLVAGLLGAVAVLCVSCVRETQ
                     RTVEIKVPGMQTERDVRIVTNAALNEAVGRATLQHGYDICVDKGTMFYYEGARLLSRE
                     YQRHILRCLREIGYEARIVEVRHWPAPAMPTPRGWLQAWPDRHAAAISIPAMKTGVDA
                     HRVVGAIARARAGDDPLRVAPDPAAHAIRVTYDTNQLALKNLEGAIANAGYQANDTPP
                     SMGLPDSFPVGWTPIWMYCPLATGESS"
     gene            11401..12066
                     /gene="lepB"
                     /locus_tag="PK634_10520"
     CDS             11401..12066
                     /gene="lepB"
                     /locus_tag="PK634_10520"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOW98209.1"
                     /translation="MWKAWGRDLAVVLGIMIPFRSSVADWNHVPSGSMKPTILECERI
                     LVNKLAYDLKVPLTTWHVVEWGQPARGDIVVFNSPRDGARLVKRVIGLPGDTVELRNN
                     RLIINGEPARYAPPDEPTGPTAPDAEMAGCELARERLGGLDHAVMAIPGTPALRSFGP
                     VVVPAGRYLVLGDNRDNSADSRYFGFVERRAIVGRASTVVVSFDPDRHYRPRFDRWFT
                     ALH"
     gene            12146..12811
                     /locus_tag="PK634_10525"
     CDS             12146..12811
                     /locus_tag="PK634_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98210.1"
                     /translation="MSGPEFKTLEEMAQATAARLSQAASHAAFHLFQDKRFRELAEFG
                     TISQEEQDRIFNELVLAFLVMIMLVLEAPDLRLPDDIRDHLDGIKDRLPRAYLDQLKE
                     MGVEERFIADWDKLIALRYEEYARDRHDVRAAAMQIESTGKDLSEHDLARIQLLVPVQ
                     SAAIGSHHHVCRGKTDGRDELFKTMLRALSHFYVELRTALEIGKIGPLARARRKLARF
                     FRA"
     gene            12876..>13103
                     /locus_tag="PK634_10530"
     CDS             12876..>13103
                     /locus_tag="PK634_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOW98211.1"
                     /translation="MVPHNLAYAKEQKAAGRKLVGIACEYAPRELILAADAMPVCLCG
                     GFEEMIAPAEKILPANLCPLVKSTFGFHLEKA"
BASE COUNT         2223 a         4320 c         4430 g         2130 t
ORIGIN      
        1 caggatatcc ccctgggtac ggatcgcgac gagctccacc gtgacgccgg gatgcagcgc
       61 ggttacggtc acaccagaac cttcgaggcg gcgcgccagc tcggtggtga agtacatgtt
      121 gcacagcttc gagtgcatgt acgttttgaa acggttgaag ttctcgcccg tgggcagttt
      181 ctcagggtcg gcctcacctt tcacatacag ccccgcattt acgttcacga tgcgcgcggg
      241 cacggacgct ttcaaccgat cgagcaacag gttcgacagc aggaacggcg cgaggtcgcg
      301 gagctcctgc tccgtgcagc cggcgaagaa ctcgatcgcc ttaagatccg ccgggtccgc
      361 catcgggcgc tccttcagcc gggagtggcc ccttgagttc ggccacctgc cggcgcaggt
      421 ccgccacctc ggcacggagc gactcgacct cggcccggag ttgcgcgagc ggatccgctt
      481 ccggggttcc ctcgatcgag gctggttcgg gtggcggcgc ggcgacggct tcgggcgcgg
      541 ccggcgctcc accgaggagg tgggcaaagc gctgctcctt gtgcccggtc tggcgcggca
      601 gtttcacgac gagcggaccc tgcggccgct cggacaggcc ctggagcacg gcctcgactt
      661 cggcctggtc ggcgaatgga tggagccgcg cggcgtgggc gcggagttgg gccgccgtct
      721 gcgggccacg gagcatcagc tcgcccatca cggccatgtc cttgctgtcc agaccgatgg
      781 tttcgacgag caattgccgg aatttcggca cgcggttgga cgtccccgtg accatggcga
      841 cgaggcgctt gtcccggagg ccctccagca ggctgtcggt catgcgatcg tcgagggcca
      901 cgaccgggaa gcggttggac ttctggttgc aggcgttggt cagggcgttg agcgagagcg
      961 ggtagtactc cggcgtcgtg atttccttct cgatcagcgc gccgaggata cggacctcgt
     1021 cggacgtcag cggcggttgc atgcgtccat cttgtcacga acgccggcgg atgacaaggc
     1081 gggccgcgtc agttcagcgc gacgatcccg gcgttcaggt acgtggcgaa caggacccag
     1141 agcaggtacg gcgcgaggag cgcggcggcg aggcggctgg cgcggcggaa gagcaggatg
     1201 accgcgacga gcgacacgtc gagcagcacg atgtccgcga gcgccaggcc gggacgctgc
     1261 aacccgaaaa agaggggcga ccacgcgaaa ttggtcagca ggtgaaacgc gaggaccacc
     1321 gtggtcacga gccggccttg gagcgggcga gcccggtagt agacgacgat cgaaacggcg
     1381 atgagcgtgt agaggatcgt ccaggccggg ccaaaaaccc acgaaggcgg cgtcagcggc
     1441 ggcagggtca gcgaggcgta ccaggcgttc atggcgcata ccatacaccg cgacgggccg
     1501 ggggcaaggg aagggttcag ggcagcttca cgcccacgcg atagaaggcg gcagggagcg
     1561 ggttggtcac cgacagggtc acggcgctgc cgtccgccgc gccggccagg ttgagtcccg
     1621 ccggcgtcca tgtcgcgtcg cccgcgagat tcgtcgtcca ccaggcttcg taccggcgct
     1681 gggcggacgt cggcaccggc gagcgcaact gcatcacgcc gcccgcctcc gcgatggcct
     1741 ggacatacga gctgaagcac gaggcggcgt tggtcggcac ggtgtcggcg acatattcct
     1801 cgaggttgtc cagcaggtcg ccgtcggaat tggcgccggc cgtggcgttc gtgaagaagc
     1861 cgaagtacag cgtctcccat tcgttgggca gtccgtcgcg gtcgctgtcc ccgcccgcgt
     1921 gcgccgccag ccggaacgag acggcgccga tctgggaggt gcgcccattg ccctgcaggt
     1981 cgcgggtgac gaaggagaag atgtagttcg tgtgcatgtc ctggaagtcg atcgtgaagt
     2041 tcaccgtctc gcgcgccggg tcgttgggcc agttcgtggc gatggtctgg agcacgaggt
     2101 tggaataccg cgcggggacg atggaaatcg tgtgcgtcag gccggtgata aacgcatcgt
     2161 tgtcccagaa atcctggtag cggaaggtca ggctcaagtt cgagcccagg tcgtaggtcc
     2221 cgccgcccgg cccggcgctg atgggattat ccgcgcggaa gggcacgacg tcgtcgccgc
     2281 tgcgctggag gaacgccagg tcgaggagct gcagctcctc gcggtccagc tcgttcacgg
     2341 gcgccagggt cgcgtgcgcc gtgcgcagca tcgccaccag gttcgtgaaa cgccgctggc
     2401 tgtgttccag gaaaggatgc gagacggcaa tgttgaagat cttcgtccgc ccgcgcacgt
     2461 ccacgctctc tccgctgacg cccagcgagc tcatcagcgc cgggtcgccg tagcgcggcg
     2521 tgccatccac gacggtcagc agcacgctcg acggccgcgc gcggatgagc gacaggtagg
     2581 gatcggcggg atccccctcg atcaccgcca ggcccgcgtt gaacccggcg gcaatctgcc
     2641 cgtaccggtt ggtcaggccc gcgcaccggg cgttgttgat cgtcaccatg tcgcacagtt
     2701 cccgcgccgt gaaggcgttg ctgaagacgg actggttcag ctcccacgcg tagcccagtt
     2761 cctcgagcag gttgaagcag ccgctgggcg tccagtccgg ggagaggccg atcaccacgc
     2821 cggcctgctt cgccgcccgg atgttggccg tgccggcgta gagcttcatg ttcgacatgg
     2881 gcgaccagat cagcttcgcg ccggccgcgg ccatctgggc gaattcgttc gtgcccagcg
     2941 ccgcgccgtg aatgatcgcc acgctcgaat tcagcatgcc ccagccgcgc caggtggcga
     3001 actgggcgcg ggcggtggtg tccgtgccct cggacaggtg aatcatggcg gcgttgacgg
     3061 ccccgcccgc gatgcggagc agcaggttcg tgcgctcggc aatgcccgac gtgttcgtca
     3121 tgcgccacgg gaaaatattg caccataccc gcgacgggaa ttcctcgatg ttgtacagga
     3181 tgacgtcggg atgggtgtgc tcgggatcgt tgttgctctg gccctggatg gcgacgcagc
     3241 cggccatgag ttccaggcac tccccgtact tggtgatcgt gtcgttcgtg gaatcccctt
     3301 ccgcggtccg aagcgctatg cgccggtttt tccagtcgtc gtattccgca tcgtcctcac
     3361 cccactggtc ccggtggccg aacgggaagt tgttgaacat cagggtcggg aacgagttat
     3421 aggccgggtg gttatgcacg tcgagcaggc ccgggaagat gatgcccccg gtgtccacgg
     3481 cggtcacgcc gggcggcgcg gcggacgtca cggcgacgat gtcgtcgtcg cgcacgtgca
     3541 cgtaccagtt cgtgcgcggg ccgcccgggg tcatcaccag gccgcgcagg gcgtactcga
     3601 agaccgtccg cccgacgagg tcgtcgtcgg agcgcggcga gatggagttg gtcgtgccga
     3661 cggaaaagac cacgccctgg attgccgaca gcacctggtt ggaaacccag acatagcggt
     3721 acggcacgcg cgtggcgacg tagaaattgg cggaggtgtc gcccgcctgc cagtaggtct
     3781 ccgtgccgcg gacgttgatg ttccgcaccg tggcgttcgt cacgcggacc agcacgccct
     3841 cgtacgcctc gttggtcacc tgccggccgg gcatccaggt cgcggcgacg ggcgcgcccg
     3901 tggaggtcag gctataggaa gacacgttgg tcagcacggt catgttgcgg gattcgcgga
     3961 cggtgccgcc gagggtcacg cggtccccgg ggttcgggcg gtggaacggg tcgttgatgt
     4021 agacgccgct ccacggcccg ccgccggcgt cggagagcgt gtagccctgc gggtcggcgt
     4081 acacggcgac gccgctgacc gtgacgctgc tgccgctgta cggcgaggcg ccgcccgcgg
     4141 cggtcgtgta ctggacgttc gataccgtca gggccgtcgc ctcccccgcc gcaagaagaa
     4201 acagaagata gccggccgcc gaccgtccgg ggaaaccgct caccgtgaaa aagctccgca
     4261 catggtccgc gtatagtacc ggattggggc ggtcttgtat aaggctatcc ggtccgttcg
     4321 ggtcgatcag aggacgcgct ggagcgcctg cttcaattcc tgcgccgccg gcgcggccat
     4381 ctcgaatacg tcctcggcgc gccggaagtc caggatgtcc acgccgcgga cggccgggcg
     4441 gatgtacaga tcgggcggcg cgcgccggag cttctccgcg atgatcgact tctgcatgat
     4501 gtcgaacgtg cccagcacgg cgcggaacag gttcgggacc ggcggcccgg cggggtcgag
     4561 gtcccccatc acgtcaaccg cgaccacgaa atcgcagtcg tccagcacgt cccacggcac
     4621 ggggttgacg ccgccgccgt cgatcagcac gcggcctgcg cgcgcgacgg gcgtgaagac
     4681 gccgggcagg cccatgctcg ccttgatcgc gtccggcagg ctgccggaat cgagcaggac
     4741 ctgctccgcc gtgttgaaat ccgtggccac gacgcgcagc gggatcttca gttcctcgaa
     4801 ggtccggacg cccatcttgc cgtagaggaa ctcgatgaac ttgtcgccct tgagcaggcc
     4861 gcggcgcccg aactcgaggt ccaggaagcc gaaccacttg agggcgtccg tcttgcgcag
     4921 ggcctcgcgc agggtatcgc gccggctgac gaccatcttc cggacgcctt cccggatgcg
     4981 ggcgccggac aggcccgagg cgtagagggc gccgaccacg gcgccgatgc tcgtcccggc
     5041 aatgcggtac ggccgcacgc ccagttcgtc gaggatctcc agcatgggga cgtgcgccag
     5101 ccccttcgcg cccccgccgc ccagcgccag cccgattttt ttcatggtat tcgctccggt
     5161 catggcttac cgcgccgccg gccggcccgc aatccgaaag tcgcggcgcg gcttgaagaa
     5221 acggcagggc ggccctatct ttacgccatg ctctaccgcc ctttcggaca gaccggcaag
     5281 cgagtctcgg tcatcggcgc cgggtgcatg cggttcgaga cgccgcagaa cgaccgggac
     5341 atcggccggg cggccgagac cctgctccac gcccacgcgc ggggcatcaa ttactttgac
     5401 accgcgccct attattgcgg ggaccggagc gaggacatcg tcggcgccgc agtccggcag
     5461 ttgaagccgg ggaccttcta cctgtccagc aagtgcgaca aggcggatgg cgcggcgttc
     5521 cggaaagggc tcgagcgctc gctgcagcgg ctcggcgtgg atcgcattca tttcttccat
     5581 atctggtgcc tgatgaatcc cacgcagtgg gcggagcgca aggaccgcgg cgcggtgcgc
     5641 gaagccctcc gggccaagga ggaaggcctg atcgaacacc tggttttctc gtcgcacatg
     5701 gacggcgagg aaacggccgc ggtgatcgcg gaggggatat tcgagggcgt gacgctcggg
     5761 tttaacgcgg ccaacttccg cttccggcgc tcggccctcg aggccgcggc ccgggccggc
     5821 ctgggcgtgg tcaccatgaa cccgctcgcc ggcggcctca tcccggccca tccgaagcgt
     5881 tttgatttta tccgggaaaa gaacgacccg gacgtggtca ccgccgcctt gcggttcaac
     5941 ctctcgcacg cggcgatcac ctgcgcgctc gtcggctttc ggaacccggc cgacgtggac
     6001 agcgccctgg ccgcgctcga cgaattcgcg ccgcggaccg acgccgagat cgcccgcgtg
     6061 gagcggcacc tggaagaggg cttcgacggc ctctgcacgg gctgcgagta ctgcctcccc
     6121 tgccccgcgg acatcccgat ccccaggatg atggactcgt acaacatgct catcctcgag
     6181 ggcgagaaga aggccgtcca ggagcggctg cactggcagt ggagcctgga ccagacggtc
     6241 gccggccagt gcaccgaatg cggggcgtgc gaggagcgct gtacgcagca cctgccgatc
     6301 atccggcggt tgaaggaaat acgcgccttg tagaaggatt gtatttgccg gttcacgggg
     6361 gaccggaatt ggagtatgct cacaatgagt ttgattcatt aaatggaggc gtatcatgag
     6421 taagcgcatg gcgttcctgg cggcgggtct tctcctcggc gcggcaacgg ttcccgcgca
     6481 ggcccagcag agcctgcagg ccctggtcaa cagcgcgacc ggcagcgtga tcgaagtgcc
     6541 ggcgggcgaa taccaggggc ccgtccggct caaggagggc gtcctcctcg tcggcgccgg
     6601 ggccgaccgc acgatcctga acgtgccgga cgccgcgccg ggcgtcatgg gcgagaacca
     6661 gtcgggcatc atcggcttca cgatccgcgg cgggcagcac ggcgtgcgga cgctcggccg
     6721 gttcatgggc gtcttcgagt gccggctgga agggcaggcc caggccgccg ttcttttcga
     6781 gggcggaagc ggcgtcgcgg tgaacaacgc gatcgtcggc tcgcagacga accagggcgt
     6841 gatgatcctc cggtcgaatc cgctggtgtc gcgcaacgtg atcgcgtacc acgatgtcgg
     6901 cgtgcacgta agcggcgaaa tgatcccacc cgtgcaggac aacctcttca tcggcaaccg
     6961 caccgccgtg ctggtcgatc ccggctcgaa ggcgctgctg gcccgcaacc tctacgaccg
     7021 caacggcgtc gccgtgcagg gcgcagacct cggggcggac gaccggatcg agcccatcgc
     7081 gtgggacggc aaggtgccgg cggccggcgc cacgacggcg cagtaccgcg acctgatgaa
     7141 cgtcgtcttc gaagaggcga tcaacgctca cccggtggtc gtgtacgggc tgggcgagga
     7201 gatgggtgcc ttcgacgtgg tgatcctctc gccctccgcg accttcagca tcggcgcgtc
     7261 ggccgtggac acgatcatct ccagccacca cgcctacgac tcggccacgc aggacgcgct
     7321 tgcttccacg ctgcgggtcg cggagcggcc gttcgtggac gtcgtcaacc cgctggtcaa
     7381 ggacctggcc gtcgaccggt acgtgctgga gaaccgctac cggcacccgg catcgtacgg
     7441 ggtgaacgaa aagggacaac gggtcttccg gcgcatgacc aacatgaacc gggccgagat
     7501 cttctgcccc gccggctggc tgcccgtcgg cgtgaattat cccgcgtcgt tcgaccgcgt
     7561 cgggaaccag gtcgtgatca agatcacgca tccgggcgtg acgaacctcg agattgcgat
     7621 ggagcccgac gccgcctcgg ccgatccgct aggggtccgc gccgcgctcg caggcgaatg
     7681 aagacccacc aggaggaaat ccgcctggag acggcgggcc atcgggacac ccacgacctc
     7741 acggatgccg ttgcggccgt cgtcgggcgc tccggcgtga cgaccggcct cgcgcatatc
     7801 ttcaacgtcg gcagcacggc ctgcgtaggc atgctcgagt ttgaacccgg cctgcagcgg
     7861 gacatcggcg aagtgctcga ccggctgatt ccgccgtcgc gcgcctacgg gcacgagcag
     7921 gcctggcacg acggcaacgg gcactcgcac ctgcaggcga cgctgatggg cccttcgctg
     7981 accgtgccgg tgagcggggg ccggctgcgg ctcggcacct ggcagcagat cttccacctg
     8041 gagtgcgacg tcgagccgcg ctcgcgcgtc gtcgtggtca ccgtgacggg ctgacccggc
     8101 cgggcggaac gcacgcggca atgatcgcgc aaacggcctc accccatcgc gcctgcttgt
     8161 gctcgccgat gccggggatg ccaacgaggt cctccacccg gccgggccgg gcgcgcgcga
     8221 gctcggccag cgtcgtatcg tggaaaatga cataggcggg gatccccagc cgctggcatt
     8281 cctcctgtcg ccagcggcgc agggtatcga acaactccat atccacccct tccatagact
     8341 tctccccgcg cgccgtgcgc ttgcgcaggc gctccttggg ctggaacagc cgcaccgcgc
     8401 gtccaccccg gagtacggcc gcggaagcct cattcagttt aagcacgggc cggtccccct
     8461 ccgtccgcgt cagcaggccc tggtccacca actggtagac cagcgcggtc agggcgctcg
     8521 cggtggagtc cttcagtaat ccgtacgtgc ttaatccgtc atggcccgcg cggcggaggg
     8581 tctccgtatc cgcgcccagc aggacgttca cgacgtgccc caccccgaac cgctggccga
     8641 cacgggcgac gcaggagagg atcttctgcg ccagcaccgt gccgtcgccg acctcttcca
     8701 cctcgtccag gcacacgtcg caggcgccgc aatccgcgcg gtcgtaggcc tggccgaagt
     8761 attccgacag cgccttatgc cggcaccgcg cggacgtgca atagcgctgc atgccgcgga
     8821 gcaactccag ttggatcttc ctcgccgcct cgaggtccgc ttcggttccc ggctccggcg
     8881 cctccgccgg cggcttgttc atgatgcctt tccagcgcat gacgtcggcg gcggaataga
     8941 acagcacgca ctcggcctcg aggccgtcgc gaccggcccg gccggtctcc tgctggtaat
     9001 gctcgatcgt cttgggcagc gaggtatgga tgacacaccg cacgttgctg cggtcgatcc
     9061 ccatgccgaa cgccaccgtg gccacgacca cgtcgagcga ctcgtcggca aaggcctcct
     9121 gcgtccggtg ccggtcctcc ggttccatgc ccgcgtggta ggcggccgcg cgcacgccgc
     9181 gggccttcaa ccaggcggcc agggtttccg tatccttgcg gctcaagcag taggcgatca
     9241 cggcctcgcc ggcgtgccgg gcgatggctt gcgcggtctg ctcgcgcacg tcggtcttcg
     9301 ggagaacacg gtagatcagg ttcgcgcggt caaaacaacc caccagcagg gccgcgtcct
     9361 tgagctgcaa ttgctcgaca atgtcctcgc gcacacgcgg tgtggccgtg gccgtgtacg
     9421 cgtgaaggct tgccgagggg aaatgctgtc gcaggacggc cagttgacga tattccggac
     9481 gaaaatcatg gccccactgg ctgatgcaat gcgcctcgtc gatggcaaac cgccgcaccg
     9541 ccagttgctt gaggtctcct agaaaatcct ccatcatcag ccgctcgggc gagacgaaca
     9601 gcaggcggat ttccgcggcg cgcaatgcgg tctccacgat ccggcgttcg gccgccgtca
     9661 tcccgctgtg cagcgccgcg gcgggatagc cgctctcccg caggccgtcc acctggtcct
     9721 tcatcagcga gatcagcggc gagaccacca cgtcgagcga gccgtccagc agcggcggaa
     9781 cctggtaaca tagcgacttc ccgccgccgg tcggcagcac cgtgagcgaa tcacattgcg
     9841 ccatggcgca cgcgatggcc tccgcctgga gcgatttcag cgattcgtat ccccaatacc
     9901 ggcgcagcgc catgttcacc tgtgcgagcg cttccgggga caacgacggc tgactcgttg
     9961 caggcatgta gatagttatt gtaccaggcc tcggactaca agcaaacccc ctcgcgccgg
    10021 cggccatcgc cgctaggact gattcacgag tgaggttatg tcccatggaa caacgggatg
    10081 ttttttccat caaaagaagc tgcggatagg gcgagaccgc cggctgagca gcatcccatg
    10141 gcgcggctca cccggagggt tcgccctacc gagaaaaacc aacgctcgag ttttcagcac
    10201 attgcgacgg cagatgaatc agtcttgcat cgccaccgct tcttacgaaa aagcatggaa
    10261 tcaagccgtc ctctttcctt tctagtaata gaagacatca cttgggccct tttactcggg
    10321 aggtgagtat gaggtagaaa actcgacggc atacaatcca atgcttcgcc ggagggcggc
    10381 cagctcttgt tcgcgtccgc ccgccagggt gcccacgcac cgcatgtccc gcagcagagg
    10441 gacatgcggt agagagtgag gcagagggtt aggcaagccc tgaaccctgt cggcgcggcc
    10501 tgtgttgggc caatctgtgc tgaaccgacc catctttagc cggtagatgt ttggccggct
    10561 ggtattttgg cgggccggtg ttgagccgac gcggggaggc ggggtacgga tggaggatcc
    10621 cggcgtgaac ggacgccaag tggaggtgtc caacaagccg ggaagggaat ccatcatgcg
    10681 actacgcttc gggctcgtgg cgggattgct cggtgcagta gcggtccttt gcgtctcttg
    10741 cgtgcgcgaa acgcagcgca cggtcgagat caaggtgccc gggatgcaga ccgagcgcga
    10801 cgtgcggatc gtgaccaacg cggcgctgaa cgaggccgtc gggcgcgcga ccctgcagca
    10861 cgggtacgac atctgcgtag acaagggcac gatgttctac tacgagggtg cgcggctgct
    10921 ctcccgggag taccagcggc atatcctgcg ctgcctgcgc gagatcggct acgaggcgcg
    10981 gatcgtcgag gtgcggcact ggccggcgcc ggccatgccc acgccccgcg gctggctcca
    11041 ggcttggccc gaccggcacg ccgcggcgat ctcgatcccc gcgatgaaga ccggcgtcga
    11101 cgcgcaccgc gtcgtcggcg ccatcgcacg ggcgcgcgcg ggggacgatc ccctgcgcgt
    11161 cgcgcccgac ccggcggccc atgccatccg cgtgacgtac gacacgaacc agctcgcgct
    11221 gaagaacctc gagggcgcca tcgccaacgc gggttaccag gccaacgaca cgccacccag
    11281 catgggcctt ccggactcgt tcccggtcgg ctggacgccg atctggatgt actgcccgct
    11341 cgccacagga gaatcctcgt gaaaactgag cgcagaccat gcaaggccca ggccgcccgg
    11401 atatggaaag cctggggccg cgatctcgct gtcgtgctcg ggatcatgat ccccttccga
    11461 tcttcggtcg cggactggaa tcatgtgccc tccggctcca tgaagccgac aatcctggaa
    11521 tgcgagcgca tcctggtgaa caagctggct tatgatctga aggtgcccct cacgacgtgg
    11581 cacgtggtgg aatggggaca gcccgcacgc ggcgatatcg tcgtgttcaa ttccccgaga
    11641 gacggcgcac gcctggtcaa gcgggtgatc ggattgccgg gcgatacggt cgagcttcgg
    11701 aataaccggt tgatcatcaa cggggagccc gctcgctatg ctccgccgga tgagccgact
    11761 gggcccacgg cgcccgacgc cgaaatggcc ggatgcgaac tggcccgcga gcgcctgggc
    11821 ggactcgacc atgccgtcat ggcgatcccc ggcacacccg ccctacgctc gttcggaccg
    11881 gtagtcgtcc ccgccggccg gtacctggtg ctgggcgaca accgtgacaa cagcgcggat
    11941 tcccgctact tcgggttcgt ggagagacgc gccatcgtcg ggcgggcgag cacggtggtc
    12001 gtgtcattcg atcccgaccg gcactatcgg ccccgcttcg accggtggtt caccgccttg
    12061 cactaatctc cgcgcgcggc ggagagagta aaagaaggat tgagagtcgg gccggcaccc
    12121 gtcaatatcc cggcagactt gaagaatgag cgggcccgaa ttcaagaccc tcgaagaaat
    12181 ggcacaggcc accgcggccc gcctgtcgca ggcggcctct catgcggcgt tccacctttt
    12241 ccaggacaag cgcttccggg aactcgcgga gttcgggacg atttcgcagg aggagcagga
    12301 ccgcatcttc aacgagctgg tgctggcctt cctcgtgatg atcatgctcg tgctcgaggc
    12361 gcccgacctg cggctgcccg acgacatccg cgaccacctg gatggaatca aggatcgcct
    12421 gccccgcgcc tacctcgacc agttaaaaga aatgggcgtc gaggagcggt tcatcgcgga
    12481 ctgggacaag ctcatcgccc tgcgctacga ggagtacgcg cgggaccggc acgacgtccg
    12541 cgccgcggcc atgcaaatcg agtccacggg aaaggacttg agcgagcacg acctcgcccg
    12601 gatacagctt ctcgtgccgg tccaatctgc ggccatcggg agccaccacc acgtctgccg
    12661 cgggaaaacc gacgggcgcg acgagttgtt caagaccatg ctgcgggccc tatcgcactt
    12721 ctacgtggag ttgcgcacgg cgctggaaat cggcaagatc ggtccgctgg ctcgcgcgcg
    12781 gagaaagctg gcccggtttt tccgcgcatg aatcgccaat ggacgcccat gccatgagtg
    12841 atagccttcc ttcctcggtc cgctggttca aggaaatggt cccgcacaac ctcgcctacg
    12901 ccaaggagca gaaggccgcc ggacgaaaac tcgtgggcat cgcctgcgag tacgctccgc
    12961 gcgaactgat cctggccgcg gacgccatgc cggtctgcct gtgcggcggc ttcgaggaga
    13021 tgatcgcgcc cgccgagaag atcctgcccg cgaacctctg cccgctcgtg aaatccacgt
    13081 tcggcttcca cctggaaaaa gca
//
LOCUS       DAOOTK010000096        13026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3202_length_13026_cov_5.175545, whole genome shotgun sequence.
ACCESSION   DAOOTK010000096 DAOOTK010000000
VERSION     DAOOTK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13026
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3202_length_13026_cov_5.175545"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PK634_10535"
     CDS             complement(<1..377)
                     /locus_tag="PK634_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOW98212.1"
                     /translation="MKKTLVIVVGIIVVLAMMLISMYNKLVNLDENAGTAWAQVETVL
                     QRRYDLIPNLVNTVKGYAAHESGLFEEITRLRSQWGEAKTTDEKAAAATQMEGALGRL
                     MVVVENCPELKANQNFMALQDELA"
     gene            complement(408..1205)
                     /locus_tag="PK634_10540"
     CDS             complement(408..1205)
                     /locus_tag="PK634_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOW98213.1"
                     /translation="MKRRRGLFLVMAGLALSVSADSDTLLGSLQPRGHVSDFAGVFEP
                     ASRDSLEQYLLSVKTLTGSEIAVVTLPSLEGGEIGDFANRLFERWGIGQKDKGALLLT
                     ALEERDVRIEVGYGLEGILTDARTGRILDESVIPRFKEGQYAAGLADGAAALARFAAE
                     ESGVSLTGAPPAGVTSTGLAALAASRPASSGGMAGLIFVFLFIGFFAALILWARKHGR
                     GGTTPSDRPRFTSSGGGISGSSGSGGSGGFSGFSGGRSGGGGASRSW"
     gene            1320..3086
                     /locus_tag="PK634_10545"
     CDS             1320..3086
                     /locus_tag="PK634_10545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOW98214.1"
                     /translation="MTNPEHEPPVPDGEWKAQLAESEQRIRQLQGQLEITTSLLRESE
                     NNLRRMAHLDALTGLFNRRGLEQELRRAWALAERQKASVGLLVIDVDRFKMLNDTHGH
                     PVGDQVLRECAELLKTGLRASDVLCRYGGDEMIVVLPLADPAETQAVATRILESVRDH
                     RFCAGRQDLRITISVGSACGRPEPGSTPDELLIEADQALYRAKQGGRNCAVFWEPRPP
                     SAEARTPASKPPADPLPPGRVMIVDDDPLLGSQMQRILETEKHDAVVHSAGRPAREQM
                     EREAGRFDVVLVDLYLKGESGWDLLQALRGQDDTLVGIVVTGAATMDNAVEALRSGAF
                     DFIAKPFEPDLLRAVVDRALRYRRLLLENRRYQQHLEELVREKSAALIRALERLRTSY
                     QFTLEALAAMLDAREKKTGEHSKRVAEMTRILARAMGVPEDQVESMSQGALLHDIGKI
                     AIPDAILLKNGPLSDAEWEVMRTHPEIGFRILRSSPELEPAAEIVRSHQERWDGWGYP
                     RGLGGSAICLGARIFAVADAYDAIRASRPYAAGQSRDAALAEIRRHRGTQFDPEVVDI
                     LARCLPDIEALGWWAEGPASAG"
     gene            3132..4646
                     /locus_tag="PK634_10550"
     CDS             3132..4646
                     /locus_tag="PK634_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW98215.1"
                     /translation="MKKWISSAIIYQVNLRSLAARDPRNAMEAFKSAAGRPPESPLAY
                     LARNLSAIKKLGANVVYLLPPYPMGIHGRKGIGSPYSSRDFYSVEPEYGTREELAELV
                     RRAHRMRLKVILDITPNHTARDHAWITSNPEFYVKAPNGEPFYDCDWSDTAKLDYTAP
                     GLRREMIEVYHHWLSFLGPDVAGQPDGVDGFRLDMAHFINDKGFWNEAMPELKARHPS
                     RQLLFLAECYGTQNNMDLFARGINAAYDDDFYKTFSYLYGVDEEGGSVICPSPDAETN
                     GDFLDKWEAFKARGIAGAVETALLNYESLLPPGEDGPFLARYTDNHDEGRGLYRAGPG
                     AVRAMMQVAFLSGHSLPFILTGQEFGALNRPSIHARIGLCDKGRRVKTPKGARVEAGL
                     EWEGNIFARTPEERSEWFRFYRDLIQLRLKNRELLRGRFQLLDAGEEVPPADRTVIAF
                     ERRLRRSALRCAVNLGPEPRQAASFCPQPGETVIYGQMPDGVLPPFSAVVLRSA"
     gene            complement(4668..8435)
                     /locus_tag="PK634_10555"
     CDS             complement(4668..8435)
                     /locus_tag="PK634_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98216.1"
                     /translation="MILPALLLAGATCRGLTLTNGRAGPGKWKDSNQCDIVSWQDASG
                     HNRTGYLVQARGCSDTNYTGGFLTRFLYRDDAGAWVQNDQIADGTLDGFGHIVNHYTP
                     PPGSRGWINSKTDAFNASNTLVFKGPHHAIWRVTFDEPALETDPAQGQWRTTLDWHFR
                     TGCDAFLYAVTFDGTGLEPGILDHDARGPYSMWDWNDDNSTFGKYGGFEWGRDFKFIC
                     TNQQQGAWTYTTPNTVPYVWEWDTNKAREIGFVQTQSQVQRAGGKTPSHGTSGTNLPY
                     YTEMGYQMNGPGMNPHTYEGITWGTPLGAIDGGDGSGSPEPYLNYSLAIVLDPLARGG
                     VTNALAEQERIHDGSVRLIASDGAVITQGMDGAGCQLAVAYDPPGYNHVYWTWDIEAS
                     MTNTAQWSLDVTNGALWNPTFVIREYRGSAPPLVVTRGGTSLVADAGYYASVDDPGNR
                     LWLTVPGVFAGSNVLRVAGPHQPDVPGVPIGLAAGNGAHGTNTVMWRDQHAVTGETYQ
                     LYHAATVITNLAEGALLTSGVPAGVEWFAHEVTSSGDHHYALVAESEYRVLNSTLVPG
                     QNATTNPAPNNDVTAPATPGNFRVRQRNNQATLSWAAVGAPDRAGYRIYRGFESGSLS
                     LYAVVTNGVSFTNRNLAPNLVYYFQVAAADDEAPPNVSAPSPELCVTSAPMTNVVVYD
                     GEPGSKPLIADQTGAWLETNTAWIAETNRAPHAGIRHLEITIVTNGQVWYGWNEGWVD
                     SDVRGYYAFNFYLRLTNDSWNNLRLQFSRAALTRSSIVYITNYLAHPATNYQLVSIPL
                     SAWKETNSLADYSFGAVSQFLFTDSGSNRVTCWMDDMLFTADNPSSTNGPVVNSAATQ
                     PAALNESNDVSEILLLASVTDRLDDIEVVLANLSAAGRSSSTIIKDDGISDDGAADNG
                     VYGCRFSLSAGAAALPGDKALTVTALDAESASGSGYATVTVRRVTFPAPILLYDGEPG
                     SRQLTNGAVWASKAQGLETNWAPYEGLNHMVCVYTHSWGEVGFAFNRAWSNINASALD
                     SLELHARTAGAVTNRPYVYFGRLGATNRSTAVFLAPAYGPVPATQYQAYVVPFTAFQE
                     TNGSGAFDFGAVSQIILGNDVGLVTSRQAYVDNIRFLYWQPDSDGDGLSDIRETGDGL
                     YINGYSTGTSSNSTDTDGDGMADGPEVWAGTDPNASGDWFRVSSVGLAGSQLQLGFGA
                     RSGRVYGIFSAGAPSGSWTSAGAVTGAGGSAAWSEPATNGSGFYRLLTAPP"
     gene            8550..11303
                     /locus_tag="PK634_10560"
     CDS             8550..11303
                     /locus_tag="PK634_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOW98217.1"
                     /translation="MRPTHARIGRGALSLCLWMAGAAPSDAGFVERAGDRTIIHLKLF
                     DLPDPTRTDAATRADAAAVREFVRRFPALFADKYRGKYRADPARYGHFNWDHVEVRLE
                     RFSGIQVEGVESDLLAIAGGMAPDVLYVNFRKSDTYIQQGFLYPLDQPGDGYLAGLGA
                     DELALRVHEKIWPVIRRRGPSGAKHVWALPYGGALGRVLLYRKDLFDEAGLAYPTKDW
                     TWEDLARAARALTQPELGRYGLAMGRGKHESWYWITFLWSAGGEVMTYDDERDAWDIR
                     FDSPAGVAALDFYTRLGAEPWTDAAGRRRRGYVYKDARDSFRKWERGEIGMVFAYVDE
                     KLFSTINPDLTGMAPVPLGPDGGRGAELNSRMMGLFSGIEHPAVRDAAWEYIRFFDSP
                     DALAIKTRVLVEGGLGRFVNPRYLEMFGYGDLARLSPPGWAETFRIAIETGRPEPYGR
                     HSNIAYNLMTQPIQEAEQMAMSGDLPADPAARAETLARLLRQAADRARSEMLDVVPPA
                     RRALQRWTAAAVLALIGVGFASVFRRIIRTFTPAAVDGVSIRPAWAFRRYAAAYLMLL
                     PAVAAILLWRYAPLARGAMMAFQDYRLLGDSAWVGLDNFGKVLWSSDWWLSLWNAFRY
                     SALVMALTFLPPVILAILLQEAPRGKVLFRMIYYLPAVMTGIVVVLLWKSFYEPGERG
                     VLNALLMRVPAAGFLGLGAILLFAALSFARRLRFHGRPGIALLFLGTGLALLWLSARL
                     AWPMLREADLPLWRRLLLTWSEPHRWLADPRTAMPACVLPMLWAGLGPGCLIYLAALK
                     SIPDELYEAAELDGATFTDKVLFVVFPMLKPLLVINFVGVFIASWYGSADTILAMTGG
                     ASGTEVAGLHIFFKAFIFLQFGPATAMAWMLGLLLIGFTVYQLRILSRIEFRAAGATE
                     AAK"
     gene            11350..>13026
                     /locus_tag="PK634_10565"
     CDS             11350..>13026
                     /locus_tag="PK634_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOW98218.1"
                     /translation="MPLLSSTGRRHPRTRLFIGAMCLLLLAGAVTMIYPFLLMVSGSV
                     KSAVDIQDLRLLPRYLVDDRALYRSYVEGLFNESIEMVRVAYDVDARSFQDVEPPAAP
                     NEKLAAEWEAFLESGRLPGTAFMLGFVEANQSRTVPEMLRRFKDSLQQQFGPDIASVN
                     RALGTDFLNWTAIFLVAENYGLRLRQVARDPFNDAFRAFKDRQPGWARAAVSLDGLYR
                     NLYLKPQYGRDIADFNRAMGTSWNSYRDIRLTASCPPGAERVPWEAFVRHTLNLLWVR
                     LDDSAAPAWRTFLAAKYGRVEAMNRLYGTAHPSFDAIPVPAEPPEGGLPLSDWDSFLK
                     GWPDPATGSLHQPPVESLRTTGPEFQFRDFLRGRYASIQAMNTALGTDFAAFDDIPLP
                     QRDAHHLAFRGIRRHLRAEFALRNYRTVLDYLLLRGRGLLNTAIYCGLSVLIALLVNP
                     LAAYALSRHRPPSAYKLLLFMLLTMAFPPIVTQIPVFLMLRGMNLLNTFAALLLPGMA
                     HGYSIFLLKGFFDSLPRELYENAELDGAGEWTIFWNITMSLSRPILAVIAL"
BASE COUNT         2025 a         4552 c         4264 g         2185 t
ORIGIN      
        1 gccagttcgt cctgcaatgc cataaagttc tggttggcct tgagttcggg gcagttctcg
       61 acgacgacca tcaggcggcc cagagcgcct tccatctgcg tcgcggccgc ggccttctcg
      121 tcggtggtct tcgcctcgcc ccactggctg cgcaggcgcg tgatctcctc gaacaagccg
      181 ctctcgtggg cggcatagcc cttgaccgtg ttgaccaggt tcgggatcag gtcataccgg
      241 cgctggagca cggtctccac ctgcgcccag gcggtgcccg cgttctcgtc caggttgacg
      301 agcttgttgt acatgctgat gagcatcatg gccaggacga cgatgatgcc gacgacgatg
      361 accagggtct ttttcatgat ctcccttccc ttggagttta cagatcgcta ccagcttcgc
      421 gaggccccac cgccacccga ccggcccccg ctgaatccgc tgaacccgcc acttcctccg
      481 ctgccgctgc tgccgctgat cccgcccccg gaggaagtga atcgcggcct gtcggagggg
      541 gtggttccgc cacgaccgtg cttcctggcc cacaggatga gcgcagcgaa aaagccgatg
      601 aaaagaaaaa caaatatcag ccccgccatc ccgccggacg atgccggacg cgaagccgcc
      661 agcgccgcca gacccgtgga cgtgacgccg gcaggcgggg cgccggtcag ggacacgccc
      721 gactcctcgg ccgcgaaccg cgccaaggcg gccgcgccgt cggccaaccc ggccgcatac
      781 tgcccctctt tgaaccgagg gataacgctt tcatccagga tacgtcccgt gcgcgcatcg
      841 gtaaggatgc cttcgaggcc atagcccacc tcgatacgca catcgcgctc ctcgagcgcg
      901 gtcagcagca gcgcgccctt gtccttctgc ccgatgcccc atcgctcgaa caacctgttc
      961 gcgaaatcgc cgatttcccc gccctcgaga gaaggcagcg tgacgacggc gatttccgag
     1021 ccggtaaggg ttttcacgga aaggagatac tgctccaggc tatcgcgcga cgcgggctcg
     1081 aagacgcccg cgaagtcgct gacatggccg cgcggttgaa ggctccccag cagcgtatcg
     1141 gaatccgccg agacgctcaa cgccagcccc gccatcacga ggaacaagcc ccgtctccgt
     1201 ttcatggcgg ccagtaaacc agagccgcga accgattcaa gcaaaagcgg agcacggatc
     1261 tctcattgta cgggctcccc tgctccgcta ggataagccc gtatccgcgg gagatgaaca
     1321 tgaccaaccc ggagcatgaa ccgccggtcc ccgatgggga gtggaaggcg caactggccg
     1381 aatccgagca acggatccgg caactccagg gacagctcga gatcaccacg tccctcctgc
     1441 gggagagcga aaacaacctg cgccgcatgg cccacctgga tgcgctgacc ggccttttca
     1501 accggagggg gctggagcag gagttgcggc gcgcctgggc cctggccgag cggcagaaag
     1561 catcggtagg cctgctcgtc atcgacgtcg atcgcttcaa gatgctcaac gatacccacg
     1621 gccatccggt cggcgaccag gtgctccgcg aatgcgcgga actgctgaag accgggcttc
     1681 gcgcgtcgga tgtgctctgc cgctacggcg gcgacgagat gatcgtggtg ttgccgctcg
     1741 ctgatccggc ggagacgcag gccgtcgcca cccgcatcct ggagtcggtg cgggaccatc
     1801 gcttctgcgc cggtcgccag gatctgcgca tcacgatatc cgtcggctcg gcttgcggcc
     1861 ggccggaacc cgggtcgacg ccggacgagt tgctgatcga ggccgaccag gcgctgtacc
     1921 gcgcgaagca gggcggccgc aattgcgccg tcttctggga gccccgtcct cccagcgcgg
     1981 aggcccggac gccggcgtcc aagcccccgg cggaccccct tcctcccggc cgcgtgatga
     2041 tcgtggatga cgatcccctc ctgggttccc aaatgcagcg gatcttggaa accgagaagc
     2101 atgacgccgt cgtccactcg gccggccgcc ccgcgcgcga gcagatggaa cgggaggccg
     2161 gccgcttcga cgtcgtcctc gtggatctct acctgaaggg ggaaagcgga tgggacctcc
     2221 tgcaggcgct gcgcggacag gatgacacgc tggtcggcat cgtggtgacc ggcgcggcga
     2281 cgatggacaa cgccgtggag gcgctgcgga gcggggcgtt cgatttcatc gccaagcctt
     2341 ttgagcccga tctccttcgg gccgtcgtcg atcgtgcgct tcgataccgt cgcctcctgc
     2401 tggaaaaccg ccgctaccag cagcacctcg aggaactggt ccgcgagaag agcgcggccc
     2461 tgatacgggc gctggagcgt ctgcgcacct cctaccagtt caccctggaa gccctcgccg
     2521 ccatgctcga cgcccgcgaa aagaagaccg gcgagcacag caagcgcgtc gcggagatga
     2581 cgcgcatcct ggcccgcgcc atgggcgtgc ccgaggacca ggtggaaagc atgagccagg
     2641 gcgctctcct gcacgatatc gggaagatcg ccatccccga cgccatcctc ctcaagaacg
     2701 gcccgttgtc cgacgcggaa tgggaggtca tgcggacgca cccggagatc ggcttccgca
     2761 tcctgcgcag cagccccgaa cttgagccgg ccgcggaaat cgtccgctcg caccaggaac
     2821 ggtgggatgg ctggggttac ccgaggggcc tcgggggatc ggccatttgc ctgggtgccc
     2881 gcatcttcgc cgtcgcggac gcctacgatg ccatccgcgc cagccggccc tacgccgcgg
     2941 ggcaatcgcg cgacgcggcc ctggccgaaa tccggcggca ccggggcacg cagttcgacc
     3001 ccgaggtcgt ggatattctg gcccgctgcc tgccggacat cgaggcgctg ggctggtggg
     3061 cggaaggccc cgccagcgcc ggatgagcga tcttgaatcc gggccggtcg ccgctacaat
     3121 cccccatcat catgaaaaaa tggatttcat cggcgatcat ctaccaggtc aacctccggt
     3181 ccctggcggc gcgcgatccg cgcaacgcca tggaggcctt caagtccgcg gccggccggc
     3241 ccccggaatc cccgctggcg tacctggccc ggaacctgtc cgccatcaag aagctgggcg
     3301 ccaatgtcgt ctacctgctg ccgccctacc ccatggggat ccacgggcgc aagggcattg
     3361 ggtcgcccta ctccagccgc gatttctact ccgtcgagcc ggaatacggc acccgggagg
     3421 aactggccga actcgtccgc cgcgcgcatc gcatgcgcct gaaggtgatc ctcgacatca
     3481 ccccgaacca cacggcgcgg gatcacgcct ggatcacgtc caacccggag ttctacgtca
     3541 aggcgccgaa cggcgagccg ttctacgatt gtgactggtc ggacacggcc aagctcgact
     3601 acaccgcgcc cggcctgcgg cgcgagatga tcgaggtcta ccatcactgg ttgagcttcc
     3661 tcggcccgga tgtcgcgggc cagcccgacg gcgtggacgg gttccgcctg gacatggcgc
     3721 atttcatcaa cgacaagggc ttctggaacg aggccatgcc ggagctcaag gcgcgacacc
     3781 cgtcccgcca gctgctgttc ctcgcggagt gctacggcac ccagaacaac atggacctgt
     3841 tcgcgcgcgg catcaacgcg gcgtacgacg acgacttcta caagaccttc tcgtacctgt
     3901 acggcgtgga cgaggagggc gggtccgtga tctgcccctc ccccgacgcg gaaaccaacg
     3961 gcgatttcct cgacaagtgg gaggcgttca aggcgcgggg catcgcgggc gccgtggaaa
     4021 ccgccctgct caactacgag agcctgctcc cgccgggcga ggacggcccg ttcctggccc
     4081 gctacaccga caaccacgat gagggccgcg gcctgtaccg cgccggaccg ggcgcggtac
     4141 gcgcgatgat gcaggtggcc ttcctgtccg gccacagcct gccgttcatc ctgaccggcc
     4201 aggaattcgg cgcgctcaac cggccctcga tccacgcccg catcggcttg tgcgacaagg
     4261 gccgccgcgt gaagacgccg aaaggggctc gcgtcgaggc cggcctggaa tgggagggca
     4321 atatcttcgc ccgcaccccc gaggaacgct cggaatggtt ccggttctac cgggacctga
     4381 tccaactgcg cctgaagaac cgcgaactgc tgcgcggccg gttccagctg ctcgacgccg
     4441 gggaggaggt cccgccggcc gaccgaacgg tgatcgcctt cgagcggcgc ctccggcgct
     4501 cggccctgcg ctgcgccgtc aacctcggtc cggaaccgcg ccaggccgca tcgttctgcc
     4561 cgcaaccggg cgaaacggtc atatacggcc aaatgcccga cggcgtgctg ccgccgttca
     4621 gcgcggtcgt gctgcggtcc gcctgatttc gcgccgcgag ggcgcgccta tggcggggcg
     4681 gtcaggagac ggtagaatcc cgaaccgttc gtcgcgggtt ccgaccacgc ggcgcttccg
     4741 cccgcgcccg tgaccgcgcc ggcgcttgtc caggatcccg acggggcgcc ggcggaaaat
     4801 atcccgtaga cccgaccgga gcgggcgccg aacccgagct gcagttgact gccggccagc
     4861 cccaccgacg acacccggaa ccagtcgccg gaagcgttcg gatcagtgcc cgcccacacc
     4921 tctggcccgt ccgccatccc gtcgccatcc gtgtccgtgc tgttcgagct cgttccggtc
     4981 gaatacccgt tgatatacag gccgtcgcct gtttcccgga tgtcggacag gccgtccccg
     5041 tcgctgtccg gctgccaata caggaaccgg atgttgtcga cgtacgcctg ccggctggtg
     5101 accaggccca cgtcgttgcc gaggatgatc tggctcaccg cgccgaaatc gaaggccccg
     5161 ctcccgttgg tttcctgaaa cgccgtgaag ggcacgacat aggcctggta ctgggtggcc
     5221 gggacaggcc cgtaggccgg ggccaggaac accgccgtcg agcgattggt cgccccgagc
     5281 cgcccgaagt acacgtaggg ccggttggtg accgcgccgg cggtccgggc gtgcagctcc
     5341 aggctatcca gcgcggaggc gttgatgttg ctccaggccc ggttgaaggc aaagccgact
     5401 tcgccccagg aatgcgtgta cacgcagacc atgtggttga gcccttcgta cggcgcccag
     5461 ttggtctcca ggccctgcgc cttcgaggcc cataccgcgc cgttggtcag ctgacgcgag
     5521 ccaggctcgc cgtcgtacag caggatcggc gcagggaacg tgacccgccg gacggtgacc
     5581 gtggcatacc ccgaaccgct tgcgctctcc gcgtcgagcg cggtgaccgt tagcgctttg
     5641 tcgcccggga gcgccgccgc gcctgcggaa agactgaagc ggcacccgta caccccgttg
     5701 tccgccgcgc cgtcatccga tatcccgtca tccttgatga tcgtcgacga cgaccgccct
     5761 gccgcggaca ggttggccag cacgacctcg atgtcgtcga gccggtcggt cactgacgcg
     5821 agcagaagga tttcgctgac gtcgttgctt tcgttcagcg cggccggctg ggtggcggcc
     5881 gaattcacga ccggcccgtt ggtcgacgac gggttgtcgg ctgtgaagag catgtcgtcc
     5941 atccagcagg tcacccggtt gctgccggaa tccgtgaaga ggaactggct cacggcgccg
     6001 aagctgtagt cggccaggct gttggtctcc ttccaggcgg agagcggaat ggacacgagt
     6061 tggtagttgg ttgccggatg ggccaggtaa ttcgtaatgt agacgatcga ggacctggtc
     6121 agcgcggccc ggctgaactg gagccgcagg ttgttccatg aatcgttggt caggcgcagg
     6181 tagaagttga acgcgtagta accgcgcacg tcggagtcca cccagccctc gttccacccg
     6241 taccagacct ggccgttggt cacgatggtg atctccaagt gccggatgcc ggcgtgcggt
     6301 gcgcggttgg tctcggcgat ccacgcggta tttgtctcca gccacgcccc ggtttggtcc
     6361 gcgatcagcg gcttgctgcc gggctcgccg tcgtacacga cgacgttcgt catcggggcc
     6421 gacgtgacgc acagttccgg cgagggcgcg ctgacgttgg gcggcgcctc gtcatcggcg
     6481 gcggccacct ggaagtaata cacgaggttg ggcgcgaggt tccggttcgt gaaggaaacg
     6541 ccgttggtca cgacggcgta cagcgacagc gagccggact cgaagccccg gtagatccgg
     6601 tacccggccc ggtccggcgc gccgaccgcg gcccatgaaa gcgtcgcctg gttgttccgc
     6661 tgccgcaccc ggaaattccc gggcgtcgca ggcgccgtga catcgttgtt aggcgccgga
     6721 ttcgtcgtcg cgttctggcc gggaacgagc gtcgagttca gcacccggta ctcggactcc
     6781 gcgacgagcg cgtagtggtg atcgccggaa ctcgtgacct cgtgcgcgaa ccactcgacg
     6841 cccgccggca cgccgctggt gagcagtgcg ccctcggcga ggttggtgat caccgtcgcc
     6901 gcgtggtaga gctgatacgt ctcgcccgtg acggcatgct ggtctctcca catcacggtg
     6961 tttgtgccgt gggcaccgtt gcccgcagcc aagccgatcg gcacgccggg gacgtccggc
     7021 tggtgcggac ccgccacgcg caggacgttg ctgccggcaa acacgccggg cacggtcagc
     7081 cacaatcggt tgcccgggtc gtccaccgag gcgtagtacc ccgcatcggc cacgagactg
     7141 gttccgccac gcgtcacgac gagcggcggc gcggagccgc ggtactcgcg gatgacgaac
     7201 gtcgggttcc aaagcgcccc gttggttacg tccaggctcc actgcgccgt gttagtcatc
     7261 gaggcttcga tgtcccacgt ccagtagacg tggttgtaac ccggcggatc gtacgcgacg
     7321 gccagctggc accccgcccc gtccatgccc tgggtgatga ccgcgccgtc ggacgcgatc
     7381 aagcggacgc tgccgtcgtg aatccgctcc tgctcggcga gcgcgttcgt cacgccgccg
     7441 cgggccaggg gatcgaggac gatcgcgagc gagtaattga gatacggctc gggcgagccg
     7501 gacccgtcgc cgccgtcgat cgcgccgagc ggcgtgcccc aggtgatccc ctcgtaggtg
     7561 tgcgggttca tcccgggccc gttcatctgg tagcccatct ccgtgtagta cggcaggttc
     7621 gtgcccgacg tgccgtgcga aggagtcttg ccgcccgccc gctggacctg gctttgcgtc
     7681 tggacgaacc cgatctcgcg cgccttgttc gtgtcccatt cccagacgta cggcaccgtg
     7741 ttcggcgtcg tataggtcca cgccccctgc tgctggttcg tgcagatgaa cttgaaatcg
     7801 cgcccccact cgaacccgcc gtacttgccg aacgtgctgt tgtcgtcgtt ccagtcccac
     7861 atgctgtacg ggccgcgcgc gtcgtgatcg aggatgcccg gctcgagccc cgtgccgtcg
     7921 aacgtgaccg cgtagaggaa cgcatcgcag ccggtgcgga aatgccagtc cagcgtcgtc
     7981 cgccactggc cctgcgcggg atcggtctcg agcgcgggct cgtcgaacgt cacgcgccag
     8041 atcgcatggt gcgggccctt gaagaccagc gtgttcgagg cgttgaaggc gtcggtcttg
     8101 gagttgatcc agccgcgcga gcccggcggc ggcgtgtagt ggttcacgat atgcccgaac
     8161 ccgtcgaggg tgccgtcggc gatctggtcg ttctggaccc aggccccggc gtcgtcgcgg
     8221 tagaggaacc gcgtcaggaa cccgcccgtg tagttcgtgt ccgagcagcc ccgcgcctgc
     8281 accaggtagc cggtgcggtt gtgcccggag gcgtcctgcc aggagacgat atcgcactgg
     8341 ttgctgtcct tccacttgcc cggcccggcc cggccgttgg tcagcgtcag cccgcggcag
     8401 gtcgcgccgg cgagcaggag cgccggcagt atcaagcgga cagaagccat agaaacagtg
     8461 taccggcgat gccccggcgg tcaaatcttt caaggccccg ccgggatcgg cgttgtgtcg
     8521 ggccggacgc tcccgtatca tgcgatccga tgaggccgac gcacgcgagg atcggaaggg
     8581 gcgcgctgtc cctctgtcta tggatggcgg gcgcggcgcc gtccgacgcg ggattcgtgg
     8641 agcgggccgg cgaccggacg atcatccacc tcaagctttt cgatctcccc gatccgacgc
     8701 gcaccgacgc ggcgacgcgc gccgatgccg ccgccgtgcg cgagttcgtc cggaggttcc
     8761 ctgccctgtt cgcggacaag taccgcggga aataccgcgc cgatcccgcc cgctacgggc
     8821 atttcaactg ggaccacgtg gaggtccgcc tggagcgttt ctccggcatc caggtcgagg
     8881 gcgtggagtc cgacctgctg gccatcgccg gcggcatggc ccctgacgtc ctctacgtga
     8941 atttccgcaa gtcggacacg tatatccagc aggggttcct gtacccgctg gaccaacccg
     9001 gggacggcta cctggccggg ctcggagcgg acgaactggc gctccgcgtg cacgagaaga
     9061 tctggcccgt gatccggcgg cgcgggccca gcggcgcgaa gcacgtctgg gccctgccct
     9121 acggcggcgc gctcggccgc gtactgctct atcgcaagga cctcttcgac gaggccggcc
     9181 tcgcctaccc gacgaaagac tggacctggg aggacctcgc ccgcgcggcc cgcgcgctga
     9241 cgcagccgga actcggccgg tacggcctcg cgatgggccg cggcaagcac gagagctggt
     9301 actggatcac gttcctctgg tcggccggcg gcgaggtgat gacctacgac gacgaacgcg
     9361 acgcgtggga catccggttc gacagcccgg cgggcgtggc cgcgctggac ttttataccc
     9421 ggctgggtgc ggagccctgg accgacgcgg ccggccggcg ccggcggggc tacgtgtaca
     9481 aggacgcgcg cgattcgttc cgcaagtggg agcgcggcga gatcgggatg gtcttcgcgt
     9541 acgtggacga gaagctgttc tcgaccatca accccgacct gaccggcatg gccccggtgc
     9601 cgctcgggcc ggacggcggg cgcggcgcgg aactgaacag ccgcatgatg ggcctgttct
     9661 ccggcatcga acatcccgcg gtgcgggacg cggcgtggga atacatccgc ttcttcgaca
     9721 gcccggacgc cctcgccatc aagacccgcg tgctcgtgga gggcggcctg ggccggttcg
     9781 tgaatccacg ctacctggag atgttcggct acggcgacct cgcccgcctc tcgccgcccg
     9841 gctgggccga gacgttccgc atcgcgatcg agaccggccg gcccgagccc tacggacggc
     9901 acagcaacat cgcctataat ctcatgaccc agccgatcca ggaggccgag cagatggcga
     9961 tgagcggcga cctgccggcc gatccggccg cccgcgcgga aacgctggcc cggctgctcc
    10021 ggcaggcggc cgaccgggcg cggtccgaga tgctggatgt cgtgccgccc gcccgccgtg
    10081 ccctccaacg gtggacggcc gccgccgtgc tcgccctgat cggcgttggg ttcgcgtcgg
    10141 tgttccggcg catcatccgg accttcacgc cggccgccgt ggacggcgtt tcgatccgcc
    10201 ccgcctgggc cttccgtcgc tacgcggccg cctacctgat gctgctgccg gcggtcgcgg
    10261 ccatcctgct ctggcggtac gcgccgctcg cgcgcggcgc gatgatggcc ttccaggatt
    10321 accggctgct cggcgactcc gcgtgggtcg gcctggacaa cttcggcaag gtcctgtgga
    10381 gttctgactg gtggctctcc ctgtggaacg cgttccgcta cagcgcgctg gtcatggcgc
    10441 tgaccttcct gccgccggtg atcctcgcga tcctgctcca ggaagcgccg cgcggaaaag
    10501 tcctgttccg catgatctac tacctgcccg ccgtcatgac aggaatcgtg gtcgtgctgc
    10561 tgtggaaatc cttctacgag cccggcgagc gcggcgtgct gaacgcgctg ctgatgcgag
    10621 tgccggcggc cggattcctg gggctcggcg cgatcctgct gttcgcggcg ctttcgttcg
    10681 cgcgccggtt gcgattccac ggccggccgg gcatcgcgct gcttttcctg ggaacgggtc
    10741 tggccctgct ctggctgtcc gcgcgcctgg cctggccgat gctccgtgag gcggacctgc
    10801 ccctgtggcg gcggctgctg ctgacctggt ccgagccgca ccgctggctc gccgatccgc
    10861 gcacggccat gcccgcgtgc gtcctcccga tgctctgggc cgggctcggc cccggctgcc
    10921 tcatctatct cgccgcgctg aaatccattc cggacgaact ctacgaggcc gccgagctcg
    10981 acggggccac gttcacggac aaggtgctct tcgtcgtgtt tcccatgctc aagcccctcc
    11041 tcgtcatcaa tttcgtcggc gtcttcatcg catcctggta tggctccgcg gacacgatcc
    11101 tcgccatgac cgggggcgcg tccggcacgg aggtggccgg gctgcacatc ttcttcaagg
    11161 cgttcatttt cctccagttc ggcccggcga cggcgatggc ctggatgctg ggcctgctgc
    11221 tgatcgggtt caccgtgtac cagctgcgca tcctctcgcg catcgagttc cgcgccgccg
    11281 gcgcgacgga ggcggccaag tgacagggac cattcactct tcaccaatca ctattcacga
    11341 ttgacaccca tgcccctcct ctcctccacc ggccgccgcc acccgcgcac gcgccttttc
    11401 atcggcgcga tgtgcctcct gctcctcgcg ggcgcggtca cgatgatcta cccgttcctc
    11461 ctgatggttt ccggcagcgt gaagagcgcc gtggacatcc aggatctgcg cctgctgccg
    11521 cgctatctcg tcgacgaccg cgccctctac cgctcgtacg tcgaggggct gttcaacgag
    11581 agcatcgaga tggtccgcgt cgcgtacgac gtcgacgcgc gctcgttcca ggacgtcgag
    11641 ccgccggcgg cgcccaacga aaaactcgcc gcggaatggg aagcgttcct cgagtccggc
    11701 cgcctgcccg gcacggcgtt catgctgggc ttcgtggagg ccaaccagtc gcgcaccgtc
    11761 cccgaaatgc tccgccggtt caaggactcg ctccagcagc agttcggacc ggacatcgcc
    11821 tcggtcaacc gggccctcgg aacggacttc ctcaactgga ccgcgatctt cctcgtggcg
    11881 gagaactacg ggctgcgcct gcggcaggtc gcgcgcgacc cgttcaacga cgccttccgg
    11941 gccttcaaag accggcagcc gggctgggcc cgcgccgccg tgtccctcga cgggctctac
    12001 cgcaacctgt acttgaagcc ccagtacggc cgtgacatcg cggacttcaa ccgggccatg
    12061 ggcacatcct ggaattcgta ccgggacatc cggctgaccg catcctgccc gccgggcgcc
    12121 gaacgggtcc cctgggaagc cttcgtgcgc catacgctga acctgctgtg ggtgcgcctc
    12181 gatgattccg cggcgccggc ctggcggacg ttcctcgccg cgaagtacgg ccgcgtggaa
    12241 gcgatgaatc gcttgtacgg gacagcgcac ccgtccttcg acgcgatccc ggtcccggcg
    12301 gaaccgccgg agggcggcct gccgttgagc gactgggatt cgttcctcaa gggctggccg
    12361 gaccccgcga cgggaagcct gcatcagccg cctgtcgaga gcctccggac cacggggccc
    12421 gaattccaat tccgcgattt cctgcgcggc cggtatgcgt ccatccaggc gatgaacaca
    12481 gcgctgggaa cggatttcgc cgctttcgac gacatccccc tgccccagcg cgacgctcac
    12541 catcttgctt tccggggcat ccgccggcac ctgcgcgcgg aattcgcgct gcggaattac
    12601 cggaccgtgc tggactatct cctgctgcgc ggccgcgggc tcctcaacac ggcgatttac
    12661 tgcggccttt ccgtgctcat tgccctgctc gtcaatccgc tcgccgccta tgccttgagc
    12721 cggcaccgcc cgccgagcgc gtacaagctg ctgctcttca tgctgctgac catggccttt
    12781 ccgccgatcg tcacccagat cccggtcttc ctgatgctgc gcgggatgaa cctcctgaac
    12841 accttcgccg ccctgctgct gccgggcatg gcccacggct attccatttt cctgctgaag
    12901 gggttcttcg attcgttgcc gcgggagctg tacgagaacg cggagctcga cggcgccggg
    12961 gagtggacga tcttctggaa catcacgatg agcctctccc ggccgatcct cgcggtcatc
    13021 gcgctg
//
LOCUS       DAOOTK010000097        12759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3292_length_12759_cov_5.825882, whole genome shotgun sequence.
ACCESSION   DAOOTK010000097 DAOOTK010000000
VERSION     DAOOTK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_12759_cov_5.825882"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK634_10570"
     CDS             <1..541
                     /locus_tag="PK634_10570"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98219.1"
                     /translation="GEADEGLVGVEAAAPVAWDIFRGLYPGGDGPWFVRPDGLKRRVV
                     CAASGCPVGSQCGHGVEDWYIAGASLFAPCPVHGRPVAAPVVSAAGRQHDAPDALRIT
                     SPAAGTVYRQLDNWSGERQQIALRAGGGAGSGELHWFADDRYLGQGAELFWPIERGAH
                     QLVCCNAVGQSDRVRIVVE"
     gene            complement(567..1631)
                     /locus_tag="PK634_10575"
     CDS             complement(567..1631)
                     /locus_tag="PK634_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98220.1"
                     /translation="MRAARHRGIMLMLALLWGTGAPRIQAEPRTWTSRTGSQVTADLA
                     GEEGERVLLRKADGQILGILFTGLSEADQEFVRRQRVPTAEAPAVENQPPAPAPALQG
                     ALTLGGVEITPGQLIRFHAPLPADAVRALDKDHNTVVTNAKVGIAVPVSFDPAKHWNI
                     IIISATSDMNSLSIGHAGQYLDAILGRNWVVMAADGPNDDAPVNVNNTWRWSLIRAGL
                     EELHRQWPASKSWSYATGGFSGGAKRSGYIAALLASSGYKVIGMFMGGCNQDMATAGA
                     NEYKPSRWTFLKVPIFLSNGLEDKVATVEAGRQVRDSMNKGGFKNIRVETCPGGHDPY
                     PEHMRQALAWFEEMAAQREP"
     gene            1844..3292
                     /locus_tag="PK634_10580"
     CDS             1844..3292
                     /locus_tag="PK634_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98221.1"
                     /translation="MAGLLAVGGAGTGWGQSDPEITNVAVEASGVVRLDGVAPAGERY
                     VMQSAYDLAQPFADVTDSTRTARVDGAVGWLLDAGGASRAFFRMKRAGTKSRLTWADL
                     EAGFAGAFGVPLAESGNYRFSYSSGVHAQLTNDNLLVVGHPYYDRQAQVQLPAILDGR
                     EGSLVGSWIDITKGLVPAGWTGGEEGSLLVGGMLLITGRLHFTKYEWYNGAGTDWQTQ
                     GYYTGGLDGSGSASGLWAVSNKYAHHSRVGGYLSQAPQALRDAGYTYLAGLEGISGAA
                     LGRWGPNLFAINSTVTNGRLRAATLICHPEEALQGPSVRCSNATSAWWVANRPANEAW
                     WIGNKVTDIKWIETDTRHGVVCFVYRGLGQTWYGLPDNGPGYPDPYGGTSGFHAEGWA
                     LQAWIYDPDEVMEVYRGERVPWNLVPAEAVLLTERLPSSSAETHYSFFTGLAVSELKS
                     SVRGNRLVVLQAGEHVINEWESTPEGYVFDLP"
     gene            complement(3319..7044)
                     /locus_tag="PK634_10585"
     CDS             complement(3319..7044)
                     /locus_tag="PK634_10585"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOW98222.1"
                     /translation="MAATIHYVATNSPSSAAPFTNWSTAAHVLQEAVDAAAAGDEIIV
                     SNGIYDSGGKVIHGAMTNRVAVDKALTVRSVNGPEATVIKGSGPNGNAAVRCVYLASG
                     SSLAGFTLTNGSTRTAGNNVTEQSGGGVFSTGGVMSNCLVTGNSAIAGGGSSCSRLYN
                     CVLAGNSANSGGGNYEGVLQNCTLTGNLGIKRAGGSSAGTLYNCIVYYNSAPVSPNYE
                     SPTMVTCCTTPAPGGTGNITNEPGLVTAWRITTDSPCVGAGSTAFARGTDMDAETWKS
                     PPSIGADEPFGSGMTGSLATAISAIYTQVAVRFVAGFTGQTAGRVAGIEWNFSDGVSF
                     SNSLHVTHAWNATGRYPVILRAWNLDYPDGIAATIHVAVLDHAFYVATNGNDENSGLS
                     WTEPKATIQGAVDAANYPGAMIWVSNGIYSTGGKVIHEPMTNRVAVDKPVGVLSVNGP
                     EVTVIQGSGPLGDAAVRCVFLTTNAMLAGFTLNNGNTRTNGDGLRQQSGGGVFSEGNG
                     IISNCLLMSNSANYGGGCSCTISINCILTGNSALYGGGSYRGTLCNSALYFNSAGEWG
                     GGSREGTLANCTLTANAANGGGGGISDGVLSNCIVYYNHAPVYSNAALSSMNYCCTIP
                     NPGEQGNITTEPGLVSAWRLSTNSVCMGAGHGEVATGTDLDGQSWRNPPCMGADEPYA
                     MGLTGNISVAISATYTQIAVGYTGGFTAHIEGLVEGNDWGFGDGIIATNRFQVSHSWS
                     ITGSYPVVLRAWNSTYPDGIRTTVQVAVLDHAYYVAPSGDDGNNGYSWTAAKATIQGA
                     VDAVDFPGAVIWVSNGVYTTGGKVMHGGLTNRVAIDKPIAVRSVNGPEVTVIRGVGPV
                     GNAAVRCVYLTNGAELVGFTLTNGHTHTDGESLDRNGGGLWCQEPSYVSNCVLSGNSA
                     SCDGGGANGGRFNNCQFIGNMAAQDGGGVRGGVLNNCFITNNSSARDGGGAADCMLTN
                     CVLKGNSTGDMGGAAAGGGLYNCILIGNASVGTHDTRGGGAFSALLVGCTVVGNSAFW
                     GGGTYSCGLNNSIIYYNIATQQSDVAQSGYEFCCTPLETEWGSDNVTNEPVFIDRANG
                     DYRLQSNSPCINRGQNQGWMTDATDMDGQPRIQRLRVDIGAYESSYWGMHSDQDNDDF
                     SDYDEVVYLGTDPTNALSCLRMEELILGEPSTAGIVIRWQSIAGKWYDVDRATNLLEY
                     PSFSTLFVDVPGQSGSTVVTDQTATASGPCIYRVNVK"
     gene            7343..8002
                     /locus_tag="PK634_10590"
     CDS             7343..8002
                     /locus_tag="PK634_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOW98223.1"
                     /translation="MHAEEPSQAPRYSRGHELANSLTHGISAALAVAGLAVLVSFAAL
                     HGNAWHVVSCSVYGTTLIALFLCSTLYHSFSNPRVKHVLRVLDHASIYLLIAGSYTPF
                     MLVNLRGPWGWSLFGVVWGLALIGIILKLFFTGRFRYASTAIYLAMGWMIVIAIRPLA
                     RVLPAGGMALLAGGGLLYTGGAVLYLFKRIPYHHAIWHLFVLAAAVAQYFAVMFYVVP
                     R"
     gene            8044..8586
                     /locus_tag="PK634_10595"
     CDS             8044..8586
                     /locus_tag="PK634_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOW98224.1"
                     /translation="MKTRKFNDEVLYPAEQPVVLDRAFVEELKRQADLNPRRRIRICA
                     HEDPGEPIHEMLIVHARMTYVRPHKHLTRIESFHVVEGEADMVMFDEQGEIARVIRMG
                     PFGSGQPFYYRLAGPVYHTLLIYTPHLVFKEVTNGPFNRADTAFPAWAPEDGQAGEAA
                     RYLAQLEARVKARRGSTWMK"
     gene            8574..9320
                     /locus_tag="PK634_10600"
     CDS             8574..9320
                     /locus_tag="PK634_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW98225.1"
                     /translation="MDEVKKEKVDVTVMVTCFNERDLVTQALDTIRTTMALFPHTYEV
                     LIYDDASRDDSVRVIREYIEKNGLQGQFELVANEKNQGIGVNYFRGAEKARGDYYLIV
                     HGDNAVPKDQIKHVMKLLGLADIIVPYYGTRLFSRKFNCDHRNFTRRFLSVCFARLVR
                     LLSGHELRYFNGLVLHRRENVVKYRVPTYGLGYQAELLCRVLNNPDVTYLEVKIHNFD
                     RASGPTSAFKRKNIISVMGSLFRIFRGRFG"
     gene            9405..10928
                     /locus_tag="PK634_10605"
     CDS             9405..10928
                     /locus_tag="PK634_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98226.1"
                     /translation="MNDKPREWMDERLFALLLTALYAWLVLGTIRVHEPWRDEAQTWL
                     IGRDASLAETFALLRYQAHPALWYLLVRPLARLGAPYAFMGLLHGLLAVGSAFLVLRF
                     APLPRLTRALLVFSFWMVWMYAVESRVYAVGILLMFLIAWRYPQRHEQPWRHGLLVAL
                     LFNSNLHMAFLAAGLSALFAWETVRARRADAAHGLALALMGAGALLLVWQFDFFRPPP
                     DNMHGGSFVWSNPPSVILRGVTTAFFPGVQNFYGWLAPLALAMMALLFWTLRARPAAL
                     FLAAAHVVGVSLLLWLTSALSRHYGLFLLGGVFALWVAADGPDAGGRADFQKQRRAFL
                     WGLNLCLAFSLYEGWKLNAMDRKHDFSGTRRMAEYIWREGLARYPIAVHRYAHLSSIA
                     PYFPGRKFWYIGRGEWVTYVRQDAAHRRANEMSHEEAAALLEQQVPAGEPLLLLTDKP
                     LKSVPPGFVPLHKVDETVRGTDEMCYLYARDVSRTGGGGLTGARSGFSTGTATTTRP"
     gene            complement(10873..11406)
                     /locus_tag="PK634_10610"
     CDS             complement(10873..11406)
                     /locus_tag="PK634_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98227.1"
                     /translation="MFTHGLLTAIVVSLAWMLLQLAAMHIRPARNRFYAMLTGYLCSL
                     PFVYAAYRWLPLPVDAQTDVEGWFGLGLFHAYLFHLLLFFLYVEFFYHVERSVTLRFL
                     VMVLQAPGRTAHPADLQQDYSLEDMITRRLADLEQNRFIERRGGRWHDRARGVLIAVI
                     MGVSSWLFRSKTQSERL"
     gene            complement(11406..11948)
                     /locus_tag="PK634_10615"
     CDS             complement(11406..11948)
                     /locus_tag="PK634_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98228.1"
                     /translation="MKGLLISALALVGYVISIWITAHLVRVERYSKLFFSLFAAWTPL
                     YFLAYFATPDHVWILPQAWSLAPWKLDAAYGYVVFALNMHNVMDFFFGVNGGFSTCLL
                     LEILRSGSRGLATEEIIARFRTADGADKIYAWRLPRLAETGYITIEPASGDCRLTAKG
                     VWAARIAWLGKRILTLGMGG"
     gene            12097..>12759
                     /locus_tag="PK634_10620"
     CDS             12097..>12759
                     /locus_tag="PK634_10620"
                     /inference="COORDINATES: protein motif:HMM:NF020013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOW98229.1"
                     /translation="MTQGDIQLTECCLCAQSAVRELADFGPQPICNRYLPRPDGPEFM
                     HPMALGQCGACGLVQLLRPVPAEEIVPPYDWIVYNEPEGHLDRLADILSGLPGLLKRG
                     VIAGVSYKDDTLLRRLRERGHRYTWRLDAHRDLDMESTGASVELLQGRISPEGATHIT
                     HGRGRSDMVIGRQVLEHSHRMRDFLEGLKRLLYPGGFVVVEVPDCTRAVENGDAAILW
                     EEH"
BASE COUNT         2241 a         4024 c         4121 g         2373 t
ORIGIN      
        1 cggcgaggcc gatgagggcc tggtgggggt cgaggcggcc gcgcccgtgg cgtgggatat
       61 cttccgcgga ctgtatccgg gcggcgacgg gccctggttt gtgcggccgg acggattgaa
      121 gaggcgtgtc gtgtgtgccg cctcgggctg cccggttggt tctcaatgcg ggcacggcgt
      181 cgaggactgg tacatcgccg gcgcatccct gttcgcgccg tgtcccgtgc atggccggcc
      241 ggtggccgcc cctgtcgtct ccgccgccgg acggcagcat gacgcgcccg acgcgctacg
      301 catcacgtcg ccggccgccg ggacagtcta tcgccagctg gacaactggt ccggtgagcg
      361 ccagcagatc gcgctccggg cgggcggggg ggccgggtcc ggcgaactgc attggttcgc
      421 ggacgaccgg tatctcggtc agggcgcgga gctgttctgg ccgatcgaac gcggcgccca
      481 ccagctcgta tgctgcaacg ccgtcggcca gagcgaccgg gtgcggatcg tcgttgaata
      541 aaatcggacg cgggccagcg acgcccctac ggctcgcgtt gggccgccat ttcctcgaac
      601 cacgcgagcg cctggcgcat gtgctcgggg taggggtcgt ggccgccggg gcaggtctcg
      661 actcggatat tcttgaaccc gcccttattc atcgaatccc gcacctggcg tccggcttcg
      721 accgtggcca ccttgtcctc gaggccgttg ctcaagaaaa tgggcacttt caggaaggtc
      781 catcggctgg gcttgtactc gttggccccg gccgtggcca tgtcctggtt gcagccgccc
      841 atgaacatgc cgatgacctt gtagccggac gacgccagca gagccgcgat gtaacccgac
      901 cgcttggctc ccccggagaa cccgcccgtc gcgtaggacc aggacttgga tgccggccac
      961 tggcggtgca gttcctccag gccggcgcgg atcagcgacc agcgccaggt gttgttgacg
     1021 ttgaccggtg cgtcgtcgtt cggaccgtcc gccgccatga ccacccagtt tcggccaagg
     1081 atggcgtcga gatactgccc ggcgtgcccg atgctcaacg agttcatgtc cgacgtggcg
     1141 ctgatgatga tgatattcca atgcttcgcc ggatcgaacg agaccggcac ggcgatgccg
     1201 accttcgcgt tggtgacgac ggtgttgtgg tccttgtcca acgcgcgaac ggcatcggcg
     1261 ggcagggggg catgaaaacg gatgagctgt cccggcgtga tctccactcc ccccaacgtc
     1321 agggcgccct gcagagcagg ggccggcgct ggaggctgat tttcaacagc gggcgcctcc
     1381 gcggtcggca cgcgctggcg gcggacaaac tcctggtcgg cctcggacaa tcccgtaaac
     1441 aggatgccga ggatctgtcc atccgccttg cgcaacagga cccgctcacc ctcttcgccg
     1501 gccaagtcgg ccgtcacctg gctgcccgtc cgcgaagtcc aggtgcgcgg ttccgcctga
     1561 atcctcgggg cacccgttcc ccagagcagg gccagcatca gcatgattcc tcgatgtcga
     1621 gcggcgcgca tattcaatcc ttccccccac aagatgccgc atggtggtat gacaactggc
     1681 gtagcgcaag gccaatctgc cgtgctgcgc ttcgaatgga ggtggcgtaa tgagataatc
     1741 agggcgttcg caccgacccc atggttgagg catgcgcaaa cggcatgata cgtgctcagc
     1801 taatatgaaa aggctcatgt tctctaggcg aatagttttg acgatggccg gtctcctcgc
     1861 ggtgggtggg gcggggacgg gctgggggca atcggatccc gagatcacga atgtcgccgt
     1921 tgaggcctcg ggcgtggtcc ggctggatgg ggtggcgccc gcgggagagc gatacgtcat
     1981 gcagtcggcg tatgacttgg cccagccttt cgcggacgtc acggacagca cgcggaccgc
     2041 ccgcgtggac ggcgccgtcg ggtggctgct ggacgcgggc ggggcgtcgc gtgcgttctt
     2101 ccgaatgaaa cgagcgggca cgaaatcaag gctgacctgg gcggacctgg aggctggctt
     2161 tgccggggcg ttcggcgtcc ccctcgcgga atcaggcaac taccgcttca gttattcctc
     2221 cggcgtgcat gcccagctga ccaacgacaa cctgctggtc gtcggccacc cgtactacga
     2281 tcggcaggcc caggtgcaac tgccggccat tctcgacggg cgcgagggct cgctcgtcgg
     2341 ctcgtggatc gacatcacga agggactcgt gcccgccggc tggacgggcg gcgaggaagg
     2401 gagcctgctg gtcggcggaa tgctgttgat caccgggcgc ctccatttca cgaagtacga
     2461 gtggtacaac ggcgcgggga ccgactggca gacgcaaggc tattacacgg gcggtctcga
     2521 cggcagcggg tcggcttccg gcctgtgggc ggtcagcaat aagtacgcgc atcattcccg
     2581 ggtgggcggc tacttaagcc aggcgccgca ggcgctgcgc gatgccgggt acacttacct
     2641 ggccgggctg gagggtattt ccggggcggc cctgggccgc tggggcccga acctgttcgc
     2701 gatcaactcc accgtcacga acgggcggct tcgggcggcg acgctgatct gccacccgga
     2761 agaggcgcta cagggaccga gcgtccgctg ctcgaacgcg acgagcgcct ggtgggtggc
     2821 gaaccgtccg gccaacgagg cgtggtggat cggcaacaag gtgaccgata tcaagtggat
     2881 cgaaaccgac acgcggcacg gcgtggtctg tttcgtctat cgcgggctcg gccagacgtg
     2941 gtacggcctg cctgataacg ggcccggcta tcccgatccg tacggcggca catcgggctt
     3001 tcacgccgaa ggctgggcgc tgcaggcctg gatttatgat ccggacgagg tgatggaagt
     3061 ctatcgcggc gagcgcgtcc cctggaacct ggtccccgcc gaggccgtgc tgttgacgga
     3121 gcggttgccg agttcttccg cggaaaccca ctacagcttc ttcaccggcc tcgctgtttc
     3181 cgagctgaag tccagcgtgc gcgggaaccg gctcgtggtc ctccaggccg gcgaacacgt
     3241 catcaacgag tgggagagca cgcccgaggg gtacgtcttc gacctgccgt aagcgagcgg
     3301 gcgccgcaag cgacgcccct acttgacatt cacacgatag atgcagggtc cactggccgt
     3361 cgcggtctgg tccgtcacaa ctgtggagcc cgactgtccg gggacatcaa caaaaagagt
     3421 ggagaaagac ggatactcca acaggttggt ggcgcggtcc acgtcgtacc attttccggc
     3481 tatgctttgc cagcggatca caatgccggc tgtagaaggt tcgcccagga ttaactcctc
     3541 catccgaagg caggagagag cgttggtcgg atccgttcca aggtaaacta cttcgtcgta
     3601 gtcgctgaag tcatcgttgt cctgatcgga gtgcattccc cagtacgatg actcgtaggc
     3661 gccgatatcc acacgcagac gttgaattcg cggctgtcca tccatatccg ttgcgtcagt
     3721 catccagccc tggttttgcc ccctgttgat gcaaggcgag ttgctctgga gccgatagtc
     3781 gccgttcgcc cgatctatga acaccggctc gttggttaca ttgtccgagc cccattcagt
     3841 ctccagtggt gtgcagcaga actcatagcc actctgggca acatccgact gttgagtggc
     3901 tatattgtag tagatgatgc tgttgttgag gccgcaagag taagtgccgc caccccaaaa
     3961 tgcagaattg cccacaacag tgcaaccaac aagaagagcg ctgaaagcac caccgccacg
     4021 cgtgtcatgc gtgccgacag aggcattgcc gatcagtatg caattatata gtccaccacc
     4081 agcggcggct ccccccatgt ccccggtaga gttccccttc aggacgcagt tggttagcat
     4141 gcagtccgcc gctccgcctc catctcgtgc ggatgaattg ttcgtgataa agcaattgtt
     4201 caacacgccg cctctgaccc caccgccgtc ttgggcagcc atgtttccga tgaactggca
     4261 attgttgaac cgccctccat tcgcgccccc gccatcacaa gacgccgagt ttcctgaaag
     4321 aacacaattc gaaacatagg aaggttcctg gcaccacaac ccgccaccgt tgcgatccag
     4381 cgactcgccg tctgtatgcg tgtgcccgtt ggtcagggta aaaccgacga gttcagcccc
     4441 atttgtgaga tatacgcacc gcaccgctgc attgccgacc ggtccgacgc ctcggatgac
     4501 tgtcacttcc ggcccgttca cactccgcac ggcaatgggc ttgtcaatgg cgacacgatt
     4561 agtaaggccc ccgtgcataa ccttgccgcc ggtagtatag accccattgc tgacccagat
     4621 cacggctccc ggaaaatcga cagcatccac ggcgccttgg atggtggcct tggccgccgt
     4681 ccagctatat ccgttgttcc catcgtctcc gctcggagcc acgtaatagg catggtccaa
     4741 cacggcaact tggactgtgg tcctgatgcc gtccggatag gtggaattcc aggcacgcag
     4801 cacaaccgga taacttccag taatgctcca agaatgactg acttggaagc gattcgtggc
     4861 aatgattcca tctccaaatc cccaatcgtt tccttccacg agtccctcga tatgagccgt
     4921 aaagcctcct gtatatccaa cggcaatctg ggtgtacgtt gcgctgatgg ccacggaaat
     4981 attgcctgtc aaccccatgg catagggttc gtctgcgccc atgcagggcg gatttctcca
     5041 tgattggccg tcaaggtctg ttccggtcgc cacttcgcca tgaccggcgc ccatgcacac
     5101 tgaattggtg gataggcgcc acgcgctcac caacccgggc tcggtggtga tgtttccctg
     5161 ttcgccagga ttcgggatcg tgcagcagta attcatggag gacagtgctg cgttcgaata
     5221 aaccggggca tggttgtaat agacaataca attgctcaac accccatctg atatacctcc
     5281 ccccccgcca ttggctgcat tggcggttaa tgtgcaattg gctagtgtgc cctcgcgact
     5341 accacctccc cactctccag ccgaattgaa gtaaagcgca gagttgcaca gagtgccccg
     5401 gtagcttcct cctccataca aggcggaatt accagtcaat atgcagttga ttgaaatggt
     5461 gcaggaacac cctcctccgt agttcgccga gttgctcatc agcaggcaat tagagattat
     5521 gccattcccc tcgctgaata cgccgccgcc gctctgctgg cgaaggccat cgccatttgt
     5581 ccttgtattc ccattgttca gagtaaaacc cgccagcatg gcgtttgttg tcagaaatac
     5641 gcaccttacg gcggcatcgc cgagcggtcc ggagccctga atgacggtta cttccggtcc
     5701 attcacgctc aacacgccca caggcttgtc aacggccact cgattggtca tcggctcatg
     5761 aataactttc ccccccgtgc tatagatgcc gttgctgacc cagatcatag cccctggata
     5821 gttggcggca tccacggccc cttggatcgt ggccttgggc tccgtccagc tcaatccact
     5881 gttctcgtcg ttgccgttcg tcgcgacgta aaatgcatgg tccaggacag ccacgtgaat
     5941 ggtggctgcg atgccatctg gataatccag attccatgct cgtaggatca cgggatacct
     6001 gcccgtagcg ttccatgcgt gcgtgacatg caggctgttt gagaaactta ctccgtcact
     6061 aaaattccat tcaatcccgg ctacgcggcc tgctgtctgc ccggtgaatc ctgcaacgaa
     6121 tctgacggcg acctgcgtat agatcgcact gatagctgtc gcaagagatc cagtcatccc
     6181 tgatccaaaa ggctcatccg cgccgatgct gggaggactc ttccaggttt cggcatccat
     6241 gtccgttcct ctggcaaacg cggtgctccc cgctcctacg caaggcgagt cggtggttat
     6301 tctccatgca gtaacgaggc cgggttcgtt agtgatgttt cctgtcccgc ccggagccgg
     6361 agtggtacaa caggtcacca tggtcggcga ttcgtagttg gggctgactg gggcgctatt
     6421 gtaataaaca atacagttat agagcgtccc agcggaactg ccgccggctc tttttatgcc
     6481 gagattgccg gtaagtgtgc agttctgaag aactccttca tagttgccgc caccactgtt
     6541 ggccgagttc ccagcaagca cgcaattata cagtcgagaa caggaacttc cgccccccgc
     6601 gatcgccgaa ttgcccgtta ctagacaatt cgacataact cccccggtgc tgaatacacc
     6661 tccaccgctc tgctccgtga cattattacc tgcggttctc gtggatccgt tagtcagagt
     6721 gaagccagct aggctgcttc cacttgccag atatacgcat cgtaccgcgg cattcccgtt
     6781 cgggccggat cccttaatca cggttgcttc agggccgttt acgctccgta ccgtcagggc
     6841 tttgtccacg gcaacgcgat tggtcatggc gccgtggatc accttgccgc ctgaatcgta
     6901 gatgccgttt gacacgatga tttcgtctcc agccgcggcg gcgtccactg cctcctgaag
     6961 gacatgagcg gcggtggacc agttggtgaa gggtgctgcc gacgaggggc tgttcgtagc
     7021 cacgtaatgg atcgtcgcgg ccacccgcga ctcggtggtc agaagtgtga tgacgctcgc
     7081 ggccacccag gccgctcccc atcgcgcacg gataccgcgg cgaggtgatg cgagcgagat
     7141 ggatatcaat tgcacggcgg ggcctcttcg cgtctcattg aactcccgag gaaagaatac
     7201 acctaccgac atggggcgga tagaccattc gcgggcttcg ttcgggcgcc ggcaagcggc
     7261 gcccctgcga gaagtttcca atgattggaa atatcggcat gaaattttcc aaacattgga
     7321 aaaccgtgct actctccgcg ccgtgcatgc cgaggaacca tcccaggccc cgcgctattc
     7381 ccgcggccac gagctggcga acagcctgac gcacgggatc agcgcggcgc tggccgtcgc
     7441 ggggctggcg gtgctggtat cgttcgccgc gctgcacggg aacgcgtggc acgtggtgag
     7501 ctgcagcgtc tacgggacga cgctgatcgc gctgttcctg tgctcgacgc tgtaccacag
     7561 cttctcgaat ccccgggtga agcatgtgct gcgggtgctg gaccacgcct cgatctacct
     7621 gctgatcgcg ggctcgtata cgccgttcat gctggtcaac ctgcgcgggc cgtggggctg
     7681 gagcctcttc ggcgtggtct ggggcctggc gctgatcggt atcatcctca agcttttctt
     7741 cacgggccgg ttccgctacg cctcgacggc gatctacctg gcgatgggct ggatgatcgt
     7801 catcgcgatc cgcccgctgg cccgcgtcct gccggcgggc gggatggccc tgctggcggg
     7861 cggcggcctg ctgtacacgg gcggcgcggt gttgtacctg ttcaagcgca ttccgtacca
     7921 ccacgcgatc tggcacttgt tcgtgctcgc ggcggccgtc gcgcagtact tcgcggtgat
     7981 gttttacgtt gtgccgcggt aaccaacctt ctatcctgtg ggccgtagcg cgtaagggaa
     8041 cccatgaaga cccggaagtt caacgacgag gtgctgtacc cggccgagca gccggtggtg
     8101 ctggaccgcg ccttcgtgga ggaactcaag cggcaggcgg acctcaaccc gcgccgccgg
     8161 atccggatct gcgcgcacga ggatcccggc gagccgatcc acgagatgct catcgtgcac
     8221 gcgcgcatga cctacgtgcg gccgcacaag cacctgaccc ggatcgagtc gttccacgtg
     8281 gtcgagggcg aggcggatat ggtgatgttc gacgagcagg gggagatcgc gcgggtgatc
     8341 cggatgggcc ccttcgggtc ggggcagccg ttttactacc ggctggcggg acccgtgtat
     8401 cacacgctgc tgatctacac gccgcacctg gttttcaagg aagtcaccaa cggtccgttc
     8461 aatcgggccg acacggcgtt cccggcgtgg gcgccggagg acgggcaggc gggggaggcg
     8521 gcccggtacc tagcgcagct cgaggcgcgt gtaaaagcga ggcgggggtc aacatggatg
     8581 aagtgaaaaa ggaaaaagtg gacgtgacgg tgatggtgac ctgtttcaac gagcgggacc
     8641 tcgtgacgca ggccctggac acgatccgca cgaccatggc gctgttcccg cacacgtacg
     8701 aggtgctgat ttacgacgac gcttccaggg acgattccgt ccgggtcatc cgcgagtaca
     8761 tcgagaagaa cggcctccag ggccagttcg agctcgtggc caacgagaag aaccagggga
     8821 tcggggtcaa ttacttccgg ggcgcggaaa aggcgcgagg cgattactac ctgatcgtcc
     8881 acggagacaa cgccgtcccg aaggaccaga tcaagcacgt catgaaactg ctgggcctcg
     8941 cggacatcat cgtcccgtac tacggcaccc ggctcttcag ccggaaattc aactgcgacc
     9001 accgcaattt cacgcgccgg ttcctctccg tctgcttcgc ccggctggtc cggctgttga
     9061 gcgggcacga gctgcgctat ttcaacgggc tggtgctcca ccggcgcgag aacgtggtga
     9121 aataccgcgt gccgacgtac gggctgggct accaggcgga gctgctctgc cgcgtgctga
     9181 acaacccgga cgtgacctac ctcgaggtga aaatccacaa cttcgaccgc gcctcgggcc
     9241 cgacctccgc gttcaagcgc aagaacatca tatcggtcat gggctcgctg ttccggatct
     9301 tccggggccg ctttggctga cgcgcccggg tgggagttgt aacaaaactg ttcacacccg
     9361 tgattaatag tgttacggat ggcgcggcgc gaaaggcggg cgggatgaac gacaaaccgc
     9421 gggaatggat ggacgagcgg ctcttcgccc tcctgctgac cgcgctgtac gcctggctgg
     9481 tgctgggaac gatccgggtc catgagccgt ggcgcgacga ggcccagacc tggctgatcg
     9541 ggcgcgacgc ctccctggcc gagacgttcg cgctgctccg ctaccaggcg cacccggcgc
     9601 tgtggtatct cctggtccgg ccgctggccc ggctgggcgc tccgtacgcc ttcatgggcc
     9661 tgctgcacgg cctgctggcg gtcggcagcg cgttcctggt tctgcggttc gctccgttgc
     9721 cccgcctgac gcgcgcgctc ctggtgttct cgttctggat ggtgtggatg tatgccgtcg
     9781 aaagccgggt gtatgccgtc ggcatcctgc tgatgttcct gatcgcgtgg cggtacccgc
     9841 agcggcacga gcagccgtgg cggcatggcc tgctggtggc cctgctgttc aacagcaacc
     9901 tgcacatggc cttcctggcg gccggcctgt ccgccctgtt cgcgtgggag accgtgcgcg
     9961 cgcgccgggc cgacgcggcg cacggcctgg ccctggccct gatgggcgcc ggcgcgctgc
    10021 tgctcgtctg gcaattcgat tttttccggc ccccgcccga caacatgcac ggcgggtcct
    10081 ttgtctggag caatccgccc tccgtgatcc tgcggggcgt caccacggcg ttcttccccg
    10141 gcgtgcagaa tttctacgga tggttggccc cgcttgcgct ggcgatgatg gcgctgctgt
    10201 tctggacgct gcgggcgagg ccggcggccc tgttcctcgc cgccgcccat gtcgtcggcg
    10261 tgagcctgct gctctggctg accagcgcgc tttcccgcca ctatgggttg ttcctcctgg
    10321 gcggggtgtt cgcgctctgg gtggcggcgg acgggccgga cgcgggcggc cgcgccgatt
    10381 tccagaaaca gcggcgcgcg ttcctctggg ggctgaacct gtgcctggcc ttctccctgt
    10441 acgagggctg gaaactgaac gccatggacc ggaagcacga cttctcgggc acgcggcgga
    10501 tggccgagta tatctggcgc gaaggcctgg cgcgctaccc gatcgccgtc caccggtacg
    10561 cccacttgag ctcgatcgcg ccctacttcc cgggccggaa attctggtac atcgggcgcg
    10621 gcgagtgggt gacctacgtg cgccaggacg ccgcgcaccg ccgggccaac gagatgtccc
    10681 acgaggaggc ggcggcgctg ctggagcagc aggtccccgc gggagagccg ctgctcctgc
    10741 tcaccgacaa gccgctgaag agcgtcccgc ccggcttcgt ccccctgcac aaggtggatg
    10801 aaaccgtccg cgggaccgac gagatgtgct atctgtacgc gcgggatgtg tcccgaaccg
    10861 ggggcggcgg gctcacaggc gctcgctctg ggttttcgac cggaacagcc acgacgacac
    10921 gcccatgatg acggcgatca ggacgccgcg cgcgcggtcg tgccagcggc cgccgcgccg
    10981 ctcgatgaac cggttctgct ccaggtccgc gagccgccgc gtgatcatgt cctccagcga
    11041 gtagtcctgc tggaggtcgg cgggatgcgc cgtgcggccg ggcgcctgga gcaccatcac
    11101 gaggaaccgc agcgtgaccg accgctcgac atggtagaag aactccacgt acaggaagaa
    11161 cagcagcagg tggaacaggt aggcgtggaa caggcccaac ccgaaccagc cctcgacatc
    11221 ggtctgcgcg tcgacgggca gcggcagcca gcggtaggcg gcgtagacga agggcaggct
    11281 gcagaggtag ccggtcagca tcgcgtagaa gcggttgcgc gccgggcgga tgtgcatggc
    11341 cgcgagctgc aggagcatcc aggccagcga aactacgatg gcggtcaaca ggccgtgcgt
    11401 gaacatcagc cccccattcc cagggtgagg attcgcttgc cgagccaggc gatccgcgcc
    11461 gcccacacgc ccttggccgt caggcggcaa tcgcccgacg ccggctcgat cgtgatgtag
    11521 cccgtctccg cgaggcgggg caggcgccag gcgtagatct tgtccgcgcc gtcggcggtg
    11581 cggaacctgg cgatgatctc ctccgtggcc aggccgcggg agccgctccg gaggatctcg
    11641 agcaggaggc acgtggagaa cccgccgttg acgccgaaga agaagtccat gacattgtgc
    11701 atgttcaggg cgaacaccac gtagccgtac gcggcgtcga gcttccaggg cgcgaggctc
    11761 caggcctgcg gcaggatcca cacgtggtcc ggcgtggcga agtaggcgag gaagtagagc
    11821 ggcgtccagg cggcgaacag ggaaaagaag agcttcgaat accgctccac gcggacgaga
    11881 tgcgccgtga tccagatgga gatcacgtag ccgaccagcg ccagcgctga aatcaggagt
    11941 cctttcatgg gcctgccgcg ccggaccgag ttccggcgac ggggcctact gtaccagccg
    12001 ccggggggcg gcacaagcgg cggccgcgtc tccgaaatgg acaaggggag ggcggccctg
    12061 tatgatgcgc caccatcacg aggctgaagt cgaagcatga cccagggcga tatccagttg
    12121 acggagtgct gcctgtgcgc gcagagcgcg gtgcgggagc tcgcggattt cggcccgcag
    12181 cccatctgca accgctatct cccgcgcccg gacgggcccg agttcatgca cccgatggcg
    12241 ctcggccagt gcggggcgtg cggactggtc cagctgctgc ggccggtgcc ggcggaggag
    12301 atcgtgccgc cgtacgactg gatcgtctac aacgagccgg aaggccacct ggaccggctg
    12361 gcggatatcc tgtcggggct gcccgggctg ctcaagcgcg gcgtgatcgc cggggtgagc
    12421 tacaaggacg acacgctgct gcggcggctg cgggagcggg gccaccggta cacctggcgg
    12481 ctcgacgcgc atcgcgacct cgacatggag agcacggggg ccagcgtcga actgctccag
    12541 gggcgcattt cgccggaggg cgcgacgcat atcacgcacg gccgcgggcg ctcggacatg
    12601 gtgatcggcc ggcaggtgct ggagcactcc caccgcatgc gggacttcct ggaggggctg
    12661 aagcggctgc tctacccggg cgggttcgtc gtcgtcgagg tccccgactg cacgcgggcg
    12721 gtcgagaacg gggacgccgc catcctctgg gaggagcac
//
LOCUS       DAOOTK010000098        12653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3324_length_12653_cov_4.970472, whole genome shotgun sequence.
ACCESSION   DAOOTK010000098 DAOOTK010000000
VERSION     DAOOTK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_12653_cov_4.970472"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK634_10625"
     CDS             complement(<1..1045)
                     /locus_tag="PK634_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW98230.1"
                     /translation="MSVKQKRSRGWVAAGLLVLNSAAAAAAVTVDEAIRAALDASPDL
                     QAVEARVDAARAMQRQARSAYYPWVDVSTQYGISDNPPQAFMMTLNQRQLNMQDPAFN
                     PNQPDDTENLRGSVALKWRLYDSGRRGADNRMAGLGAEAAGEMLSAARNQLIHEVTRG
                     CHGALQARAFAAVQEEAVASIEASLRAANARFEAGSAVKTDVLNLETQLAQAREDLIR
                     ARNGLLLAVAALNTAIGRDLVTEDNLEEGAEPPEEKCAAPDPSAVENRPELAAARRMV
                     RVREQALSKARREYGPALNAFASSDWDSDASSDFEQSYTVGVMAELNVFDGFRSRGAV
                     AAARAELDAARAEE"
     gene            complement(1159..2439)
                     /locus_tag="PK634_10630"
     CDS             complement(1159..2439)
                     /locus_tag="PK634_10630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOW98231.1"
                     /translation="MKQVPILMPQMGQSVAEGTIIKWLKQVGETIAVDEKLLEVETDK
                     TTVEVESPAAGRLAACLKREGEIAAAGEIIGLIESEAAADALALAPAVPPSVEKGASA
                     PGSSAPPSVFNGHAELPEISRDYYSPYVLRLAMQNNVSIAELQTIRGTGRHGRITRTD
                     LLRHLARRPVGAMSVSALAGTPEVKADDLAELGEIVPMTSLRRTIADHMVQSIHTSAH
                     VMMVHAVDVTHIVELRNRIKDDFFNKHNAKLTYTAVMLFVTSRVLRDFPKINASIYGT
                     NIILRKEINIGCAVALADETLVVPVVRQADRMSFPEVAKELSRLIGVARAKALKRADV
                     EGATFTISNFGSFGSLIGTPIIHQPQVAILGMGAVFKAPVVLDGQVVVRDQLYLSFSF
                     DHRIIDGEQGGRFLKAIEKATGALTEEALSVDRL"
     gene            complement(2450..3433)
                     /locus_tag="PK634_10635"
     CDS             complement(2450..3433)
                     /locus_tag="PK634_10635"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOW98232.1"
                     /translation="MPTREMTYLEAIRQGLRRLLRERPEVVVYGEDVGAFGGAFKVTL
                     GLQAEFGADRVFDTPISESAIFGSAIGMATQGLRPIVELQFVDFGMTALHQLMNNAAT
                     THYRTGTAVPLTVRAPCGGGFGGGPFHSEELEAFFCHMPGLKVVYPSTPSDARGLLVS
                     AAMDPNPVIYLENKFLYRHVKEMLPEAFEPVPLGVARVCRPGRDAVILTYGAMVHEAL
                     RAAERLATEEGHDVMVLDLRTLKPCDADTIVAAVAATSRVLILHEGWRTGGFGAELAA
                     LVAERAFHLLDAPVLRVAAPDCPVPFAPELESAYRPDADRLCEALVELMDY"
     gene            complement(3420..4412)
                     /locus_tag="PK634_10640"
     CDS             complement(3420..4412)
                     /locus_tag="PK634_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOW98233.1"
                     /translation="MKSESQNSAGGIPRESWEAVARCMWRARLTEERLVRLYHQGRIF
                     GGVYTGIGQEAIGAATACAGGPDDLFAPCIRNMTVHLARGMSVLDIFRQYLGRATGPT
                     RGRDGNVHYGNLANGVYAMISHLGAMLSVVTGGVMARRRLGRPAVGVAYIGDGATSTG
                     DFHEALNFASVFDAPVLFVIENNKFAYSTPSSSQYRCARLVDRAAGYGMEGLVADGND
                     AVGLHRLARELLARMREKPRPVLLECDTMRMRGHGEHDDCSYVPKELAERYAARDPLR
                     LAREGLAAEGALDAAAFDRIEKDCQAEVDAGYQQALAEPAPDPATLTRGVYADA"
     gene            4650..5489
                     /locus_tag="PK634_10645"
     CDS             4650..5489
                     /locus_tag="PK634_10645"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOW98234.1"
                     /translation="MDAGPAYSREEEGRLGRRPVLGGYRVRLAMMQSYFFPYIGYFQL
                     LASVDRMILYENLNHIRHGWVHRNRIRLKGRDAVYCSVPLLHASSFVKIRDLLVDRSQ
                     RWPGKFLDLLTFNYRKARFFEEVYPVIEEAVSREAARLAEVNWNAFTAVASFLGIAAE
                     IRRDTPPCDEFEAAVARKDAGWFRALGQSLGTDDVRQQRVLYVCRAEGADAFHNPIGG
                     LELYSRDLFGRNGIRLRFLRPRLTPYEQSESAFIPGLSILDVLMHCGREGTRQQLADY
                     DIV"
     gene            5529..7397
                     /locus_tag="PK634_10650"
     CDS             5529..7397
                     /locus_tag="PK634_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98235.1"
                     /translation="MSPAFASLGLMAFGSAFVVAYLLTPLAERLAPRLGLMDVPGARH
                     AHGRPTPRAGGLAVFLGFHAGCALLYFLSAPGLEGDLTRGWWLRFLAVSSVLLAVGLA
                     DDAWRLRPLLKLAGQTVAALLAYALGIRFHVPVPVPAPELVDLALTLAWFLAIINAFN
                     LIDGIDGLATGLALIASLGLMVSLVLRWRPGDVGVVLALAGTCLAFLRYNFHPARIFL
                     GDGGSQFLGLALAAVSLSTASKSAVISSLLVPLLAVGVPLFDTLLAIWRRGVRHRGRV
                     GAILEGDADHLHHRLARAGLSQRQVAMLLYLISGALILVGLLLMTLRSYVIGISLTAF
                     VGGVYVVVRHLARVELWDTGAAILRGLRRPPRQAVCVLLYPLMDVLIMAGALAINIRL
                     FAETPAGAFAGRLLKYSVIWVAVPFLILIVAGTYRRVWSRAGAAEYATLAAALGGGLL
                     LAAGLANVLLGFAAREVLEYLPVYGGMTLLILIGLRGFRPVVMDCLAFRSPRRAGDQP
                     LRRLLLYGAEDRAMLFLREHRYAALENPPREQIVGLLDDDSNLHGRLIHGYRVLGGLD
                     FFQKQAASLGVDELVLVKDLDPETTARLLETAGRHGIAVSEWRTERRRLDGGGGQG"
     gene            7394..8620
                     /locus_tag="PK634_10655"
     CDS             7394..8620
                     /locus_tag="PK634_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98236.1"
                     /translation="MSGLSPRGQALFDGFQKVAPGAADYFRPWTFDVPLAIRKETSDR
                     LRRVQQLYLKCIRRFAEHYDRYRDLMPVPDRVTEILALCRRKPYRPGTYRTDFVIDES
                     NRARLIETTCRFAMNGYFTSGFFVHNLADRWLAGRPAIRKIDEYTAFYERYMDSFGPF
                     DHVCLLKGADERNDTKFVIPVFEAAGFPVRIIRTAEVPARAGEFAGAAVLGELSHEEL
                     CGLPTETIKAIIDADSMNDLRTVFLFHDKRFFALLYHDAFAREALTAAELDELRACVV
                     PTYTKRLNPELWPRARDDKDRWIIKPHNMGKSIDVHAGCVTDPAEWRALFDSGRADRM
                     VLQEYVPQRRFRGTIGDKACHDYVVGTLLFFEDGYFGPGLCRASSFPVTNKVDDRKVA
                     PLVTDDLSAFEADNVI"
     gene            8626..10098
                     /locus_tag="PK634_10660"
     CDS             8626..10098
                     /locus_tag="PK634_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOW98237.1"
                     /translation="MSLRQQTVSGLLWSFLDDLARGGLLFLSGLVLARLLTPREFGLI
                     GMTTLFIAISQSIVNSGFNQALIRKQDCTQADYSTVFYFNLLVSFALYLLLFFAAGWI
                     ARFFREPPLEGIIRALGLGLLIGALGMVQQTQVVKEIDFKLLTRISLVSSAIGGALGI
                     ALAYAGYGVWSLVVMTLSRAAVELALLWRWRSWRPSLEFRWASFRAMFSFGSRLLASS
                     LITTLYTQLFNVVIGRFFSAAELGQFTQAQTFRNLPSQRLTAVVQRVSYPALATLQEE
                     APRLKAAYRRMLQCTMLATFALMLGMAACAEPLLITLVGEQWRPAIVYLQLLCFVGMF
                     YPLHAINLNMFQVRGRSDLYLKLEVIKTVLAAPAMAVGVFWGIQPMIVTLFFISLAAY
                     VLTAACSGRLIGYSAAEQIRDILPSFILAAVMAAAVYVLGRWLPLPDLPRLVVQVGAG
                     ATLTVGLAELTRLDSYLYLKRLALDKLMGGPKPSPGKAGA"
     gene            10243..11139
                     /locus_tag="PK634_10665"
     CDS             10243..11139
                     /locus_tag="PK634_10665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98238.1"
                     /translation="MTAGNRPKPADSRSAPLVSVKVPAYNHERYIEACLAGILAQKTA
                     FPFEVVIGEDSSTDGTRGIVEAYAKAQPEVIRVVTSESNVGPARNLARIRAACCGEYE
                     AMCEGDDLWIHPRKLQRQVEFLEAHPDYVFCFHDTLFYREDKRARPRYYAPPDLPESP
                     TVTDVLQRPPFIATSSILARRSFLDALPAWRTQVLCGDLVVRLWGPHAGRIGYLNAIM
                     ALRRRHTGGLSVQTGHRRMAEEAVKAYRLFDEATGGRYARLVRDRIAFERQYARLGTL
                     CYLWRPSRARDRFRAVRSGAGL"
     gene            11154..11936
                     /locus_tag="PK634_10670"
     CDS             11154..11936
                     /locus_tag="PK634_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW98239.1"
                     /translation="MQRQAKPVKPSSDYDAFASVYDRHWGQHSLKKFAPILADRLRPL
                     LPAGARVLDVCCGTGQIARWFSGQGFRVTALDRSQAMLDHARARAPAAEFVLADARDF
                     SLPPVHSAAVSMFDSLNHILSPDELGAAFRCIHAALAPGGVFFCDFNMRNKFAGGWKG
                     DFNIVEDDLACIVRTQYDDASRLARWDVTVFRRRHTWKRTDLVFTQRAYEEQEVQQAL
                     ADAGFSSIRAWDALHPPDGGTPLPPGKTYFIAVKTDEIGRPA"
     gene            11933..>12653
                     /locus_tag="PK634_10675"
     CDS             11933..>12653
                     /locus_tag="PK634_10675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOW98240.1"
                     /translation="MKIFVDALSRIPTVSIGTQALIIGGLHILRRRFPDVTFVMLTSH
                     PAQERHYLDATGYPIEYVERAPSQWGTLRQFHAIVRGVDAVACAWGDGYVGKPAWRLL
                     QKTLSLKRTGVPLILVTASLGPFRAGLDAWFARRALSLFDRLTVRDLNSQRHIRNLDL
                     EDVRCLPDTAFVLEPAPEAAVDNILRREGVPGGEAPVGVNPSILLHHRYTAVNGRPYP
                     PVVARLIDHIRRTTRRPVLLIP"
BASE COUNT         1799 a         4329 c         4391 g         2134 t
ORIGIN      
        1 cctcctcggc ccgcgccgcg tcgagctcgg cgcgcgcggc ggcgaccgcc ccgcgcgaac
       61 ggaagccgtc aaagacgttc agctctgcca tcacgccgac ggtgtagctc tgctcaaaat
      121 cgctggaggc gtcgctgtcc cagtccgacg aagcgaaggc gttgagcgcg ggcccgtact
      181 cgcgccgcgc cttgctcaag gcctgctccc tgacgcggac catccggcgc gcggcggcca
      241 gctcgggccg gttctccacg gcggacgggt ccggagcggc gcatttctcc tccggcggtt
      301 cggccccctc ttcgagatta tcctccgtca ccaggtctcg gccgatggcc gtgttgagcg
      361 cggcgacggc caggagcaag ccgttgcgcg cgcggatcag gtcctcgcgg gcctgggcca
      421 gttgggtttc caggttcagc acgtcggtct tcaccgcgct gccggcctcg aagcgcgcgt
      481 tcgccgcgcg caggctggcc tcgatgctgg cgacggcctc ctcctggacc gcggcgaacg
      541 cgcgcgcctg gagcgcgccg tggcagccgc gggtcacctc gtggatcagt tggttgcggg
      601 cggcggagag catttccccg gccgcctcgg cgccgagccc cgccatacgg ttgtccgccc
      661 cgcgccgtcc gctgtcgtag aggcgccact tgagcgcgac gctgccgcgc aggttctcgg
      721 tgtcgtccgg ctgattggga ttgaaggcgg ggtcctgcat gttcagctgg cgctggttca
      781 gggtcatcat gaaggcctgc gggggattgt cgctgatccc gtactgggtc gaaacatcaa
      841 cccagggata gtaggccgac cgcgcctgcc gctgcatggc ccgggccgcg tccacgcggg
      901 cctcaaccgc ctggaggtcc gggctggcgt ccagcgccgc gcggatcgcc tcgtccactg
      961 tcaccgccgc ggccgccgcg gccgagttca ggaccagcag ccccgccgcc acccatcccc
     1021 tgctccgctt ttgcttcacg ctcatcgccc gctcctttgt tcatcccgtg tcgccaatat
     1081 attagaatat catgatatgt caatatgtat gttgcggttt tttttgctcc cgcgggaggg
     1141 ggtggcggcg cggccgggct acagccggtc gacgctcagg gcttcctcgg tgagcgcgcc
     1201 ggtggccttc tcgatggcct tgaggaaccg gccgccctgc tcgccgtcga tgatccggtg
     1261 gtcgaagctg aagctcaaat agagctggtc ccggacgacg acctgcccgt ccagcacgac
     1321 gggcgccttg aagaccgcgc ccatccccag gatcgcgacc tgcggctggt ggatgatcgg
     1381 cgtgccgatg aggctgccga aggagccgaa attggagatg gtgaacgtcg cgccctcgac
     1441 gtccgcccgc ttcagggcct tggcgcgggc gacgccgatc agccggctca attccttcgc
     1501 cacctccggg aagctcatgc ggtcggcctg ccgcacaacg ggcaccacga gcgtctcgtc
     1561 cgccagcgcc acggcgcagc cgatgttgat ctccttgcgg aggatgatgt tggtcccgta
     1621 gatggacgcg ttgatcttcg ggaaatcgcg gagcacccgc gaggtgacga acagcatgac
     1681 cgcggtatag gtcagcttgg cgttgtgctt gttaaagaag tcgtccttga tccggttgcg
     1741 cagctcgacg atgtgcgtga cgtccacggc gtgaaccatc atcacgtgcg cgctggtgtg
     1801 gatggactgg accatgtggt cggcgatggt ccgccgcagc gaagtcatgg gcacgatctc
     1861 cccgagctcg gcgaggtcgt cggccttgac ttcgggcgtg cccgcgaggg cggacacgga
     1921 catcgccccg acgggccgcc gcgcgaggtg acgcaggagg tccgtcctcg tgatccggcc
     1981 gtggcggccg gtgccgcgga tcgtctgcaa ttcggcgatc gacacgttgt tctgcatcgc
     2041 gaggcgcagg acgtagggcg agtagtagtc ccggctgatc tcgggcagct cggcatggcc
     2101 gttgaacacg gagggcgggg cggacgaccc gggcgcggag gcgcccttct cgaccgacgg
     2161 cgggacggcg ggcgcgagcg ccagggcgtc ggcggccgcc tcggactcga tgaggccgat
     2221 gatctcgccg gcggcggcga tctcgccctc gcgcttgagg caggcggcca gccggcccgc
     2281 ggccgggctt tcgacctcga cggtggtctt gtcggtctcg acctcgagca gcttctcgtc
     2341 cacggcgatg gtctcgccga cctgcttgag ccacttgatg atggtgccct cggccacgct
     2401 ctggcccatc tgcggcatga gaatcggcac ttgtttcatg gtccggccct caatagtcca
     2461 tcagttccac gagcgcctcg cagagccggt cggcatccgg ccggtaggcg ctctccagtt
     2521 cgggggcaaa ggggaccggg caatcggggg cggccacgcg gaggacgggg gcgtcgagca
     2581 ggtggaaggc gcgctccgcg accagcgcgg cgagctcggc gccgaacccg ccggtgcgcc
     2641 agccctcgtg gaggatcagc acgcggctgg tggccgcgac ggcggcgacg atggtgtcgg
     2701 cgtcgcaggg cttgagcgtg cgaaggtcga gcaccatgac gtcgtgcccc tcctccgtcg
     2761 cgaggcgctc ggccgcgcgc agggcctcgt ggaccatggc gccgtaggtc aggatcaccg
     2821 cgtcgcggcc gggccggcac acgcgcgcca cgccgagggg cacgggctcg aaggcctcgg
     2881 gcagcatctc cttcacgtgg cggtacagga acttgttctc caagtagatc acggggttgg
     2941 gatccatcgc ggcggagacg agcagcccgc gcgcgtcgga cggggtggac gggtagacca
     3001 ccttgaggcc gggcatgtgg cagaagaagg cctcgagctc ctccgaatgg aagggcccgc
     3061 cgccgaaccc gccgccgcag ggggcgcgga cggtcagggg gacggccgtg ccagtccggt
     3121 agtgcgtggt cgcggcgttg ttcatcagct gatgcagcgc ggtcatgccg aagtcgacga
     3181 actggagctc gacgatcggc cgcaggccct gggtcgccat gccgatcgcg gagccgaaga
     3241 tcgcggactc gctgatcggc gtgtcgaaga cgcggtccgc gccgaactcc gcctgcaggc
     3301 cgagcgtgac cttgaaggcc ccgccgaacg cgccgacgtc ctcgccgtag accacgacct
     3361 cggggcgctc gcggagcagg cggcggagcc cctgccggat ggcctcgagg taggtcatct
     3421 cacgcgtcgg cataaacccc ccgggtcagc gtggccgggt ccggcgcggg ctcggcgagg
     3481 gcctgctggt agccggcgtc cacctcggcc tggcaatcct tttcgatccg gtcgaacgcg
     3541 gcggcgtcca gcgcgccctc cgcggccagg ccctcgcggg cgaggcgcag cgggtcgcgg
     3601 gcggcgtatc gctcggccag ctccttcggg acgtaggagc agtcgtcgtg ctcgccgtgc
     3661 ccgcgcatgc gcatggtatc gcactccagc aggacgggcc gcggcttctc ccgcatccgc
     3721 gcgagcagct cgcgggcgag ccggtgcagg ccgaccgcgt cgttgccgtc ggcgaccagc
     3781 ccttccatgc cgtagccggc cgcgcggtcg acgaggcgcg cgcagcggta ctggctggag
     3841 ctgggcgtgg aataggcgaa cttgttgttc tcgatgacga agaggacggg ggcgtcgaag
     3901 acggaggcga agttgagcgc ctcgtggaaa tcgcccgtgc tggtcgcgcc gtcgccgatg
     3961 taggcgacgc cgacggccgg ccggccgagc cggcgccggg ccatcacgcc gccggtaacg
     4021 acggagagca tggcgccgag gtgggagatc atcgcgtaca cgccgttcgc gaggttgccg
     4081 tagtggacgt tgccgtcgcg gccgcgggtc gggccggtcg cgcggccgag gtactgccgg
     4141 aagatgtcca gcacgctcat gccgcgggcc aggtggacgg tcatgttgcg gatgcacggc
     4201 gcgaagaggt cgtccggccc gccggcgcag gccgtcgcgg cgccgatcgc ctcctggccg
     4261 atgccggtgt agacgccgcc gaagatgcgg ccctggtggt agaggcgcac caggcgctcc
     4321 tcggtcagcc gggcgcgcca catgcagcgg gcgacggcct cccacgactc ccgtggaata
     4381 ccgcccgcgc tgttctggga ctcggatttc actgaaaacg cggggtaaac tgtagcccct
     4441 cccgtccggc gttgccaagg caaaacgggg ccgcccgcgg ggcgaggcgg cgggcgtttt
     4501 ccagacattg gaaaaatcgg ggcgtttttt tccaatgttt ggaaaaaatt cccgtgtttt
     4561 ttccaaacat tggaaaattc cgccggcttt tttccaatcc ttggaaaacc gcgggcggag
     4621 acggcgcggg gcggcggccg gacctcgaca tggacgcagg ccccgcctat agtcgggaag
     4681 aggaaggccg gctcggccgg cggccggtcc tcggaggata tcgcgtgcgc ttggcgatga
     4741 tgcagtcgta tttttttccg tacatcggat atttccagct gctcgcctcc gtcgacagga
     4801 tgatcctgta cgaaaacctg aaccacattc ggcacggatg ggtgcaccgc aaccggatac
     4861 gcctcaaggg gcgcgacgcg gtgtactgct cggttcccct gctccacgcc agttcgttcg
     4921 tgaaaatccg ggacctgctc gtggaccggt cgcagcgatg gccgggcaag ttcctggacc
     4981 tgctgacctt caattacagg aaggcgcgct ttttcgaaga ggtctacccg gtgatcgagg
     5041 aggccgtgag ccgcgaggcg gcgcgcctgg cggaggtcaa ctggaacgcg ttcaccgcgg
     5101 tggcctcctt cctcggcatc gcggccgaga tccgccgcga cacgccgccc tgcgacgagt
     5161 tcgaggcggc ggtggcgcgg aaggatgccg ggtggttccg cgccctgggc cagagcctgg
     5221 gcacggacga cgtgcggcaa cagcgcgtgc tgtacgtgtg ccgcgccgag ggggcggacg
     5281 cgttccacaa tcccatcggg ggcctggagc tgtattcccg ggacctgttc gggcgcaacg
     5341 ggatccgcct ccggttcctg cggccgaggc tgacgcctta cgagcagagc gaaagcgcgt
     5401 tcatcccggg gctctccatt ctcgacgtgc tgatgcactg cgggcgggag gggacccggc
     5461 aacaactggc ggattatgac atcgtctaga cggtgcccgg cagggcggcg gcgcacggga
     5521 ggccgggcat gagtccggct ttcgcgagcc tcgggctgat ggccttcggc agcgcgtttg
     5581 tcgtcgcgta cctgctcacg ccgctggcgg agcggctggc gccgcggttg gggctgatgg
     5641 acgtgcccgg cgcccggcat gcgcacgggc gccccacccc ccgcgccggc gggctcgcgg
     5701 tgttcctggg ttttcatgcg ggctgcgcgc tcctgtactt cctttccgcg cccggcctcg
     5761 agggggacct gacgcggggc tggtggctcc ggttcctggc cgtgtcctcg gtcctgctgg
     5821 ccgtgggcct ggcggatgac gcgtggcgcc tgcggccgct gctgaaactg gccgggcaga
     5881 cggtcgcggc gctcctcgcc tatgccctcg gcatccggtt ccacgtcccc gtgccggtcc
     5941 cggcccccga gttggtggac ctggcgctga cgctggcgtg gttcctcgcg atcatcaacg
     6001 cgttcaacct gatcgacggc atcgacggcc tggccacggg gctggcgctg atcgcctcgc
     6061 tgggcctgat ggtttcgctg gtgctgcggt ggcggcccgg ggacgtcggg gtcgttctcg
     6121 cgctggccgg gacgtgcctc gcgttcctcc gctacaattt tcatccggcg cggatcttcc
     6181 tgggcgacgg cgggagccag ttcctcgggc tggcgctggc ggcggtgtcc ttgagcacgg
     6241 cctcgaagag cgcggtgatc tcgtcgctgc tggtgccgct cctggccgtc ggggtgccgc
     6301 tgttcgacac cctgctggcg atctggcggc gcggcgtccg gcatcgcggc cgcgtgggcg
     6361 cgatcctcga gggggacgcc gaccacctgc accaccgcct ggcccgcgcg ggcctgtccc
     6421 agcggcaggt ggccatgctg ctctacctga tcagcggcgc gctgatcctg gtcgggctgc
     6481 tgctcatgac gctccgctcg tacgtgatcg ggatctccct gaccgcgttc gtcgggggcg
     6541 tgtacgtggt ggtccggcac ctggcgcgcg tcgagctctg ggacaccggc gccgcgattc
     6601 tgcgcggcct gcgccggccc ccgcgccagg cggtgtgcgt gctgctgtac ccgctgatgg
     6661 atgtgctgat catggcgggg gcgctggcga tcaacatccg gcttttcgcg gagacgccgg
     6721 ccggggcctt cgcggggcgc ctcctgaagt acagcgtgat ctgggtcgcc gtgccgtttc
     6781 tcatcctgat cgtggcgggg acctaccgcc gggtctggag ccgggcgggc gccgcggagt
     6841 acgcgacgct cgcggccgcg ctgggcgggg gcctgctgct ggccgccggc ctggcgaacg
     6901 tcctcctggg cttcgcggcc cgcgaagtgc tggagtacct gccggtctac gggggcatga
     6961 cgctgctcat cctgatcggc ctgcgcggct tccggccggt cgtgatggac tgcctggcct
     7021 tccggtctcc gcggcgcgcc ggcgaccagc ccttgcggcg cctgctgctc tacggggccg
     7081 aggaccgggc catgctgttc ctgcgcgagc acaggtatgc cgccctggag aacccgccgc
     7141 gggagcagat cgtgggcctg ctcgacgacg actccaacct gcacggccgc ctcatccacg
     7201 gataccgcgt gctgggcggg ctggatttct tccagaagca ggccgcgtcg ctgggcgtgg
     7261 acgagctggt cctcgtcaag gacctggacc cggaaaccac cgcccggctg ctggagaccg
     7321 ccggccgcca cggcatcgcg gtcagcgagt ggcggaccga gcggcgacgg ttggacggcg
     7381 ggggaggcca gggatgagcg ggttgtcgcc acgcggccag gcgctcttcg acgggtttca
     7441 aaaagtcgcc cccggggccg ccgactactt ccggccgtgg accttcgacg tcccgctggc
     7501 gatccggaag gaaacgagcg accgcctgcg ccgcgtccag cagctctacc tgaagtgcat
     7561 ccggcgcttc gcggagcatt acgaccgcta ccgcgacctg atgcctgtcc ccgaccgggt
     7621 caccgagatc ctggcgctct gccggcgcaa gccgtaccgc cccggcacct accgcaccga
     7681 cttcgtcatc gacgaatcca accgggcgcg gctgatcgag accacgtgcc ggttcgccat
     7741 gaacggctac ttcacctccg ggttcttcgt tcacaacctc gcggaccgct ggctggccgg
     7801 acggccggcg atccggaaaa tcgacgagta cacggcgttt tacgaacgct acatggactc
     7861 tttcggcccg ttcgaccacg tctgcctgct caagggcgcc gacgagcgga acgacaccaa
     7921 gttcgtcatc ccggtcttcg aggccgccgg cttcccggtg cggatcatcc gcaccgccga
     7981 ggtcccggcc cgcgcgggcg aattcgcggg cgcggccgtg ctgggagagc tgtcgcacga
     8041 ggagctgtgc ggcctgccaa cggagaccat caaggccatc atcgacgcgg actccatgaa
     8101 cgacctgcgc accgtgttcc tgttccacga caagcgcttc ttcgccctgc tctaccatga
     8161 tgcgttcgcc cgggaagcgc tcacggccgc ggagctcgac gaactccggg cctgcgtcgt
     8221 ccccacgtac accaagcggc tgaatcccga gctctggccg cgggcgcggg acgacaagga
     8281 ccgctggatc atcaagcccc acaacatggg caagagcatc gacgtgcatg ccggctgcgt
     8341 gacggacccc gccgagtggc gggccctgtt cgactccggc cgggcggacc ggatggtcct
     8401 gcaggagtat gtgccccagc gccggttccg cggcacgatc ggggacaagg cctgccacga
     8461 ttacgtggtg ggcaccctcc tctttttcga ggacggctat ttcgggccgg gcctgtgccg
     8521 ggcgtcgtct ttcccggtga ccaacaaggt ggacgaccgg aaggtcgcgc cgctggtgac
     8581 cgacgacctc tccgccttcg aagcggacaa cgtcatctga ccgacatgtc gctaagacaa
     8641 caaacggtgt ccggcctgct gtggagcttt ctggacgacc tggcccgggg aggcctcctg
     8701 tttttgagcg gcctggttct cgcgcgcctc ctgaccccgc gcgagttcgg gctgatcggg
     8761 atgaccacgc tcttcatcgc gatctcgcag tccatcgtca acagcgggtt caaccaggcc
     8821 ctgatccgga aacaggactg cacgcaggcg gattattcca cggtcttcta cttcaacctc
     8881 ctggtcagtt tcgcgctgta cctgctgctg tttttcgcgg ccggctggat cgcgcgcttc
     8941 ttccgcgagc cgccgctcga aggaatcatc cgggcgctcg gcctgggcct tctgatcggc
     9001 gcgctgggca tggtgcagca aacgcaggtc gtcaaggaaa tcgatttcaa gctgctgacc
     9061 cggatttcgc tcgtatcgtc cgcgatcggc ggggcgctcg gcatcgccct ggcgtatgcc
     9121 ggctacggcg tctggagcct ggtcgtcatg acgctttccc gcgccgcggt cgaattggcc
     9181 ctgctgtggc ggtggaggtc gtggcggccg tcgctggaat tccgctgggc ctccttccgg
     9241 gccatgttct ccttcggatc ccgtctgctg gccagcagcc tgatcaccac gttgtacacc
     9301 cagctcttca acgtcgtcat cgggcggttc ttttccgcgg cggaactggg gcagttcacc
     9361 caggcgcaga ccttccggaa cctgccgtcg cagcggctca ccgcggtcgt tcagcgggtg
     9421 agctatcccg cgctcgccac tctgcaggaa gaggcgcccc gcctgaaggc cgcgtaccgc
     9481 cggatgctgc aatgcacgat gctggccacc ttcgcgctga tgctggggat ggcggcctgc
     9541 gccgagcccc tgctgatcac cctcgtgggc gaacagtggc ggcccgccat cgtctacctg
     9601 caactcctgt gcttcgtggg aatgttctat cccctgcacg ccatcaacct gaacatgttc
     9661 caggtgcggg gccgcagcga tttgtacctg aagctcgagg tgatcaagac ggtcctggcg
     9721 gcgccggcga tggccgtcgg cgtcttctgg ggcattcagc ccatgatcgt caccctgttc
     9781 ttcatcagcc tcgccgccta cgtcctgacc gcggcctgct cgggccggct catcggctac
     9841 agcgccgccg aacagatccg ggacatcctg ccctcgttta tcctggcggc ggtgatggcg
     9901 gcggcggtgt acgtcctggg acggtggctg cccctccccg acctgcccag gctggtcgtg
     9961 caagtcggag caggcgcgac gttgaccgtc ggcctggcgg agctcacccg cctggacagt
    10021 tacctttacc tgaagaggtt ggcgctggac aaactcatgg gaggtccgaa gccctccccc
    10081 ggaaaggccg gggcatgaaa ctggcgcgcg cggcgttacc tcctccgcca aaagaagttg
    10141 tgcgcgcagc gaccggttga caagaatgcg gcaatttgaa agtgtacact cccgtggtga
    10201 ggcagcaccg acgaaaacga tgatgtcaca ggagatggaa ttttgacagc gggaaatagg
    10261 cccaagccgg ccgatagccg gagcgcgccg ctggtcagcg ttaaagtgcc cgcctataac
    10321 cacgaacggt atatcgaggc gtgcctggcc ggcatcctgg cccagaaaac ggccttcccc
    10381 ttcgaggtgg tcatcggcga ggatagcagc acggacggca cgcgggggat cgttgaggcg
    10441 tatgcgaagg cgcagccgga ggtcatccgg gtcgtcacgt ccgaaagcaa cgtcgggccg
    10501 gcgcgcaacc tcgcgcgcat ccgggcggcc tgctgcggcg agtacgaggc catgtgcgag
    10561 ggcgacgatc tctggatcca cccgcgcaag ctccagcgcc aggtcgagtt tctcgaggcg
    10621 catcccgact acgtgttctg ctttcacgac acgctgttct accgcgagga caagcgcgcg
    10681 cggccccggt attacgcgcc cccggacctg ccggaatcgc ccacggtgac cgacgtgctg
    10741 cagcgtccgc ccttcatcgc cacgtccagc atcctggcgc ggcggtcgtt cctggatgcg
    10801 ctgcctgcct ggcggaccca ggtcctgtgc ggcgacctgg tcgtccgcct ctggggcccg
    10861 cacgcggggc ggatcggcta tctgaacgcg atcatggctc tccggcgccg gcacacgggc
    10921 ggcctgtccg tgcaaaccgg ccaccggcgg atggccgagg aggccgtcaa ggcctaccgg
    10981 ctgttcgacg aggccacggg cggccgctat gcccgcctgg tccgggaccg catcgccttc
    11041 gagcggcagt atgcccggct tggaacactc tgctacctgt ggaggccgtc gcgggcgcgg
    11101 gaccggttcc gcgccgtacg ctcgggcgcg ggactctaac acgcgggaaa ccgatgcaac
    11161 gacaagccaa accggtcaag ccgtcctcgg actatgacgc ctttgcctcg gtttatgacc
    11221 ggcactgggg acagcacagc ctgaagaagt tcgcgccgat cctcgcggac cggctgcggc
    11281 ccctgctccc ggccggggcc cgggtgctgg acgtctgctg cggcacgggg cagatcgcgc
    11341 ggtggttctc gggccaggga ttccgggtga ccgccctcga tcgctcccag gccatgctgg
    11401 accatgcgcg ggcccgggcg ccggccgccg agttcgtgct cgccgacgcg cgggacttct
    11461 cgctgccgcc cgtccactcg gcggccgtct ccatgttcga cagtctcaac catatcctct
    11521 cgcccgatga actgggcgcg gccttccgat gcatccatgc ggccctggcg ccggggggcg
    11581 tgttcttctg cgacttcaac atgcggaaca agttcgccgg cgggtggaaa ggcgatttca
    11641 acatcgtcga ggacgacctc gcctgcatcg tgcggacgca atacgacgat gcctcgcgcc
    11701 tggcccgctg ggacgtgacc gtctttcgcc ggcgccacac ctggaagcgc accgacctgg
    11761 tcttcaccca gcgcgcgtac gaggagcagg aggtccagca ggccctggcg gacgccgggt
    11821 tcagcagcat ccgggcatgg gacgccctcc accctcccga tggcggcacg cccctgcccc
    11881 cgggcaagac ctatttcatt gctgtcaaga ccgatgaaat cggaaggccc gcatgaagat
    11941 tttcgtcgat gccctgtcgc gcatcccgac cgtgagcatc gggacgcagg ccctgatcat
    12001 cggcggcctg cacatcctgc gccgccgctt cccggacgtg accttcgtca tgctgaccag
    12061 ccacccggcc caggagcgcc actacctcga cgccacgggc tatcccatcg agtacgtgga
    12121 gcgggcgccc tcgcagtggg gcacgctccg gcagttccac gccatcgtgc ggggcgtgga
    12181 cgccgtggcc tgcgcgtggg gcgacgggta cgtcggcaag ccggcatggc ggctcctgca
    12241 gaagaccctt tccctcaagc ggacaggcgt gccgctgatc ctcgtcaccg cctcgctcgg
    12301 ccccttccgc gcggggctcg atgcctggtt cgcgcgccgg gcgctctcgc tcttcgaccg
    12361 gctcacggtc cgcgacctga attcccaacg gcacatccgg aacctggacc tcgaggacgt
    12421 ccgctgcctg ccggatacgg ccttcgtcct ggaaccggcg ccggaggccg ccgtggacaa
    12481 catcctgcgg cgcgaggggg tgcccggcgg cgaggcgccc gtcggcgtga accccagcat
    12541 cctccttcac caccgctaca ccgccgtcaa cgggcggccc tacccgcccg ttgtggcgag
    12601 gctgatcgat cacatccgcc ggacaacccg gcgcccggtc ctgctcattc ccc
//
LOCUS       DAOOTK010000099        12362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3410_length_12362_cov_5.170066, whole genome shotgun sequence.
ACCESSION   DAOOTK010000099 DAOOTK010000000
VERSION     DAOOTK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12362
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_12362_cov_5.170066"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="xseA"
                     /locus_tag="PK634_10680"
     CDS             <1..839
                     /gene="xseA"
                     /locus_tag="PK634_10680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOW98241.1"
                     /translation="IQDMLNILGRRFPNLYILVAPVKVQGEGAAAEIAAAIDTLNARG
                     GLDVLIVGRGGGSLEDLWSFNEEVVARAIARSALPVISAVGHETDFTISDFVADLRAP
                     TPSAAAELVVRPKAEFEQWLAQWAGRLARGLRQRALELKNRLLKVERSDVFREPRSLV
                     KQYRQRIGALHAGLAREVRHRLQRRQQRLDDLGLRFRKQALVWRDAKKQDLRRLAASL
                     KALSPDAVLERGFSITRSADGRVLRDAARVAAGERISTKLHRGTLESEVVKGGAHGGK
                     EK"
     gene            820..1068
                     /locus_tag="PK634_10685"
     CDS             820..1068
                     /locus_tag="PK634_10685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOW98242.1"
                     /translation="MAEKKNDQKMTFEKALARLEAIVAEMESGQLSLEKMMEVFEEGS
                     GLVKFCSGQLNEVERKIEQLIKKGDGTETAPFEAAAAK"
     gene            complement(1089..1736)
                     /gene="hypB"
                     /locus_tag="PK634_10690"
     CDS             complement(1089..1736)
                     /gene="hypB"
                     /locus_tag="PK634_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOW98243.1"
                     /translation="MATQNIKVYKDLMGENEKWAAATRRVLAERRLSMVNLIGSPGSG
                     KTALIEATAKKLGAGFRAAVLEGDVETTRDAERIAAVNLPVSQLLTSGACHLEAKLVY
                     QALLELPTENLRLVIVENVGNMVCPAEFDIGEHAKVAVLSVTEGEDKPLKYPLLFREA
                     KAVVLTKIDLLPHLDFNLAACEGFIQQVHAGVPVFKVSARTGEGLSAWADWLLGV"
     gene            complement(1717..2085)
                     /locus_tag="PK634_10695"
     CDS             complement(1717..2085)
                     /locus_tag="PK634_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOW98244.1"
                     /translation="MHEFSIGQSVVQTVLAELSGRGLPPGALRAARIAVGSMHQIVPD
                     TLVFAYEALVRDTPAAGSRLVIRPVPVTARCKACGWQGALEVPLFLCGACQSGEIELI
                     TGMELHLESLEVEEHGDAEH"
     gene            complement(2067..2540)
                     /locus_tag="PK634_10700"
     CDS             complement(2067..2540)
                     /locus_tag="PK634_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOW98245.1"
                     /translation="MSAAIIVIGLGNPLMADEGVGVRLVERLAAQSGRFPGVKFLDAG
                     TSGMVALHAMRGLRKAVFVDCALMGEAPGAIRRFTPDQAASLKKLPGLSLHEGDLFGI
                     LELSKQLGECPPEVVIFGIQPSNLEPAPGLSDTLTARLPEYEAAVRAEISCTSSP"
     gene            complement(2626..3924)
                     /locus_tag="PK634_10705"
     CDS             complement(2626..3924)
                     /locus_tag="PK634_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008526674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOW98246.1"
                     /translation="MSKTIDIHVHHITRVEGHGNIVLRASDGRIEKLEWQVPEAPRFF
                     EAMVRGRSFEDIQTIVSRICGICSVTHSFSCIKAIEDAMGIVVSEQTDKIRMLLHYSE
                     QLESHSLHVGYLVAPDLLGQKSVVPLVKSHPDVVKTVIKLHRTGNQWMEAIGGRMTHP
                     VTIKPGGFASLPTEQYLRELKKTLEGIVPDLKALAEVVLSLAPKLPAFDRDTEYVALV
                     DPTHYSFYHGGIGSTDTKDLTPVRKFESVVNEYVTAQSTAKWCKWHRDAFAVGSLARF
                     NLNADLLLPLAAATAKKLGLKKGCCNPYMNNVAQVVESVQVVEHSLQIIDELLTKGIQ
                     PEVPKVQPREGEGAGAVEAPRGILFHRYAFDKKGHCIRANCCIPTNQNHANIQKDFEA
                     LVPQIIGRPQEEIRLLLEMLVRAYDPCISCSTHYLDVKFV"
     gene            complement(3945..4700)
                     /locus_tag="PK634_10710"
     CDS             complement(3945..4700)
                     /locus_tag="PK634_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98247.1"
                     /translation="MDKPRVAIFDFACCEGCQLQIVNLEEELLDVLSLVQPVEWREAI
                     SDQSDTYDIAIVEGSITRPGDEEKLRQIRSRAKVLVALGACATMGGVNKLKNNFTMED
                     VRREVYGKDGSMPHLDTAPTKALDEVVKVDLKIHGCPINNKEFAYILRCLALGKTPAI
                     PNYPVCVECKMRGNVCRYEYNEICLGPVTRAGCDAKCPSAGFWCFGCRGYVDDPNVQA
                     AREVMEHYGKTMEDLKGRLLLFNSKQEATENVK"
     gene            complement(4700..5542)
                     /locus_tag="PK634_10715"
     CDS             complement(4700..5542)
                     /locus_tag="PK634_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOW98248.1"
                     /translation="MSQILTAEKDIYLPEPADVVRAEPMTKMEMFFEFKLSSGRPLGH
                     MPGQFAEVAIPGIGEAPISISSSPTYRSGFQMVVRNVGNVSAAMHRLAPGKKVGIRGP
                     FGTQFPVDGAMKGQDLLFVCGGIGLVPVRSAIDYVLHHRKDYGRVTILFGTKSPAERL
                     FVEEAKQWAAQPDTTFLETVDRGDEHWKGSVGVITTLMPKVKVDPARTVAVICGPPIM
                     YKFVILELDKMGIGKDRIFVSLERHMKCGVGKCGHCQINGLYVCQDGPVFRYTDVAEV
                     REAI"
     gene            complement(5539..6564)
                     /locus_tag="PK634_10720"
     CDS             complement(5539..6564)
                     /locus_tag="PK634_10720"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOW98249.1"
                     /translation="MSIKKIGPAALERWVDGLIGRQTVIGVQAKGDKFAFAPLAKTSA
                     LRLDYDVAVTAPKAFLQPPCETLLRFNPKGYESVVDEQPFVLFGVHPYDAAAILQMDV
                     VFAEGQADIHYLKRRAQATLVVSDVQQPSANVFAGCMNTAVLEQGGDVMLTRIGDAYL
                     VESRSDKGAALVKSLAGEPDAAPADLQLRKKTWDDNRKRLRRHELKVKPGDLPALLAR
                     SYDHPVWEEKSKRCFSCGSCNLVCPTCYCFNVRDDVRWDLQSGERVRTWDGCMLAEFA
                     LVAGGHNFRKKKPERYRHRYLRKGQYLFEQHRQIACVGCGRCIGACVTKIANPVEIYN
                     RLAEGPR"
     gene            6735..7451
                     /locus_tag="PK634_10725"
     CDS             6735..7451
                     /locus_tag="PK634_10725"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOW98250.1"
                     /translation="MAVRGFPALALLAVTGGCSGASEHPPQEEGMTDKAVGMVEVLKA
                     YGLRDPDVLRAMGKVRRPEYIPERYRDGSDPWGDHPCSIGHGQTISQPFIVAYMTERM
                     APWKGEKFLEIGTGSGYQAAVLAELGAEVFTIEIIPELAEHARRALAAEGYARVNVRA
                     GDGYRGWPEHAPFDAILVTCAPEEIPPALVDQLKDGGRMILPVGEAGAQRLVILRKKD
                     GRVTVEDDLPVRFVPMVRGR"
     gene            7557..8810
                     /locus_tag="PK634_10730"
     CDS             7557..8810
                     /locus_tag="PK634_10730"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOW98251.1"
                     /translation="MSSTPATETEYLPQGPSNVLVINAGSSSLKFTMYAMRGETIRAK
                     GIVERIGLADPCLVYERFDGKKLKEKVSVRNHDEALRVICDRLVDPAVGVLANLGEVE
                     AIGHRVVHGGEKFRDSVRITDEVKRIIQEMATLAPLHNPPNLGGIEACAHVFGDIPNV
                     AVFDTAFHQSMQPSSYLYAIPYEYYEKFGVRKYGFHGTSHKFVAQATAAYLRKPMARL
                     KLITCHLGNGCSIAAIDRGKVIDTSMGMTPLNGLVMGTRCGDIDPAVVLYLVRQGMSA
                     DEVDRMLNKRSGLLGVAGIGSGDMRDIIAAAESENDQAKRALFMFFHRLVSYIGAYET
                     ILDGADAVVFTGGIGENSAYVRERVVSRLGCLGCFLDKAANEAAVGRQAVISTAESIL
                     KAVVMPTNEELMIARETVRLLSPAA"
     gene            8827..9837
                     /gene="pta"
                     /locus_tag="PK634_10735"
     CDS             8827..9837
                     /gene="pta"
                     /locus_tag="PK634_10735"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOW98252.1"
                     /translation="MSFLDTLIPKARAAARTLVLPEGHDPRVMEAAAKIAKQGIAARV
                     IVLATPAERAKTRDQVSFEGLPVQVLDYLNAPERERLMLKLLELRRAKGMTPEQARDT
                     IQNRLYFGNMMVREGLADGMVAGSIASTPDMVRTAFQCVGTAPGIKFGSSCFIMDLAR
                     PTPGGQGVLLYADSGVNPNPTAEQLVDITVATIRTCQALVGGQARVAMLSFSTRGSAK
                     HDLVDKMVRATDLAKKRVAELGLDALVDGELQADAAIVPAVAASKCADSPLKGAANIL
                     IFPDLQAGNISYKLTERLAGATAYGPIMQGLAKPVNDLSRGCSADDIVGVAAITVCQA
                     IG"
     gene            10231..11214
                     /locus_tag="PK634_10740"
     CDS             10231..11214
                     /locus_tag="PK634_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW98253.1"
                     /translation="MDAAIITTYRCPNRCHMCNVWKFPTKVEEEFKPGLLEKLPRLRF
                     ANVTGGEPTVREDLDEILAVLRRKAERVVISTNGFLTERLLGLAERHPGIGIRISIEG
                     LPAANDELRGVKDGFDHGLRTLLELQRRGVKDIGFGITVSDRNAKDMLELYQLAKSMD
                     VEFATAAVHNSYYFHKYDNRIEKQDEVIACFRELVRELLRTRKIKNWYRAYFNHGLIQ
                     FVRGQPRLLPCEAGTENFFLDPWGEIRPCNGMEEGVWCESMGNLHAQTFEGIWNGPKA
                     AAVREKVKHCPKNCWMIGTASPVMKKYIRVPTLWVLRNKWRVLTGREPCTD"
     gene            11224..12348
                     /locus_tag="PK634_10745"
     CDS             11224..12348
                     /locus_tag="PK634_10745"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98254.1"
                     /translation="MDGSIAYPAGLLVDLPLEGPPVLACGADGKAAPCAAQGGRAFLA
                     EPVGNLAEYEAAARYRATVEALLDRLGIKASVVAHDLHPDYESTLFARAFPADRRIAV
                     QHHHAHAAACMAEHGLTGPVIGVALDGSGYGPDGTVWGGEFLVADYAGFRRAGHFKPY
                     RLPGGEGAIRNPPRMALSWLAAECGEAADALAARWLPAIPGEQRTMLLGLVASGAHSP
                     WTSSAGRLFDAASALLGLCDRIAHEGDAAIRLQAAADPRETGEWPFALEEAVLSFGPA
                     LHALLEQRAAGVAIPVLAARFHRTVAAGAAALCGRLREREKPNRVVLSGGVFQNDLLR
                     AWLTETLSASGFEVYCPRRVPPGDAGVALGQAAVASASGW"
BASE COUNT         1973 a         4112 c         4195 g         2082 t
ORIGIN      
        1 cgatccagga catgctgaac atcctgggcc ggcggttccc gaacctgtac atcctcgtcg
       61 cgccggtgaa ggtgcaaggc gaaggggccg cggcggagat tgcggcggcg atcgacacgc
      121 tcaacgcgcg cggcgggctg gacgtgctga tcgtcgggcg gggcgggggg agcctcgagg
      181 acttgtggtc cttcaacgaa gaggtcgtcg cgcgggcgat cgcgcggtcg gccctgccgg
      241 tgatctccgc ggtcgggcac gagacggact tcaccatcag cgattttgtc gccgacctgc
      301 gcgcgccgac gccgtccgcg gcggcggaac tggtcgtccg gcccaaggcg gagttcgaac
      361 agtggctggc ccagtgggcg gggcggctgg cccgcggcct gcggcagcgc gcgctggagc
      421 tgaaaaaccg gctgctcaag gtcgagcgca gcgacgtgtt ccgcgagccg cgcagcctgg
      481 tgaagcagta ccggcagcgg atcggggcgc tgcacgcggg cctggcccgc gaggtccggc
      541 atcggctcca gcggcggcag cagcggctgg acgacctggg cctgcgtttc cggaagcagg
      601 cgctcgtatg gcgcgacgcg aagaagcagg acctgcgccg gctggcggcc tcgctgaagg
      661 ccttgagccc ggacgccgtg ctggagcggg gcttcagcat tacgcggtcc gcggacggac
      721 gggtcctgcg ggacgccgcc cgggtggcgg cgggcgagcg gatttcgacg aaattacatc
      781 gcggcactct ggaatccgag gtcgtgaagg gaggggcgca tggcggaaaa gaaaaatgac
      841 cagaaaatga ctttcgagaa ggcgttggcc cggctcgagg cgatcgtggc cgaaatggag
      901 agcggccagt tgagcctgga gaaaatgatg gaggtcttcg aggaaggctc cggcctggtc
      961 aagttctgtt ccggccaact caacgaggtc gagcggaaga tcgagcaact catcaagaag
     1021 ggggacggca cggagaccgc gccgttcgag gctgctgcgg ccaagtagga aggccgctcg
     1081 tggccgatct acacgccgag cagccagtcg gcccaggccg acaatccctc gccggtccgg
     1141 gcggatacct tgaacaccgg cacgccggcg tggacctgct ggatgaatcc ctcgcaggcc
     1201 gcgaggttga aatcgaggtg cgggagcagg tcgatcttcg tcagcacgac cgccttcgcc
     1261 tcgcggaaaa gcaggggata cttcagcggc ttgtcctcgc cctcggtcac gctcaagacc
     1321 gcgaccttgg cgtgctcgcc gatgtcgaat tccgcggggc agaccatgtt gcccacgttc
     1381 tcgacaatga ccagccgcag gttctccgtc ggcagttcca gcagggcctg gtacacaagc
     1441 ttcgcctcga ggtggcacgc gccgctggtg aggagctggc tgacgggcag gttcacggcg
     1501 gcgatgcgct cggcgtcgcg cgtggtttcg acgtcgcctt ccagcacggc cgcgcggaac
     1561 ccggcgccca gcttcttcgc cgtggcctcg atcagcgcgg tcttgccgga gcccggcgag
     1621 ccgatgaggt tgaccatgga caggcgccgc tcggcgagga ccctgcgcgt ggcggcggcc
     1681 cacttctcgt tctcgcccat caggtccttg tagactttaa tgttctgcgt cgccatgttc
     1741 ctccacctcc aggctttcca ggtgcagttc catccctgta atcaactcga tttcgcccga
     1801 ttggcaagcc ccgcacagga agagagggac ctccagcgcg ccctgccagc cgcaggcctt
     1861 gcaccgcgcc gtgacgggca ccgggcggat gaccagccgc gacccggcgg ccggcgtgtc
     1921 ccggacgagt gcttcgtagg cgaaaaccag cgtgtccggc acgatctggt gcatggaacc
     1981 gacggcgatc cgggccgcgc gcagggcgcc gggcggcagg ccgcggccgc ttaattcggc
     2041 caacacagtc tggacgacgc tttggcctat ggagaactcg tgcatgagat ttccgcccgg
     2101 acggccgcct catactccgg gaggcgggcg gtcagcgtat ccgagagtcc gggcgcgggt
     2161 tcgaggtttg acggttgaat gccgaagatg acgacctccg gcgggcactc gcccagttgt
     2221 ttcgacaact ccaaaatgcc gaacaggtcg ccctcgtgga gggaaagccc cggcaacttc
     2281 ttcaggctgg cggcctggtc cggggtaaag cgccggatcg cgccgggggc ttcgcccatc
     2341 agggcgcagt ccacgaagac ggcctttcgc agcccgcgca tggcatggag ggcgaccatg
     2401 ccggacgtgc cggcgtccag aaattttacg ccgggaaagc ggccggattg agcggcgagc
     2461 cgttcgacca gccgcacgcc gacgccttca tcggccatca ggggattgcc gagcccgatc
     2521 acgatgatgg ccgcgctcat cctccttgtc cttctctttc tccttctccc cctctccggc
     2581 ccatcggagc cgggcacgag aacgaggaag agaagaaaaa agctcctaca cgaacttcac
     2641 gtccaggtaa tgcgtggagc aggagataca cgggtcgtag gcgcggacga gcatctcgag
     2701 gagcaggcgg atttcctcct gcggccggcc gatgatctgc ggcaccaggg cctcgaaatc
     2761 cttctggatg ttcgcgtggt tctggttggt cgggatgcag cagttggccc ggatgcagtg
     2821 gcccttcttg tcgaaggcgt agcggtggaa caggatgccg cgcggcgcct cgacggcgcc
     2881 ggcgccctcg ccttcgcggg gctggacctt cggcacctcg ggctggatgc ccttcgtcag
     2941 cagctcgtcg atgatctgga ggctgtgctc gacgacctgc acgctctcga cgacctgggc
     3001 gacgttgttc atgtaggggt tgcagcagcc cttcttcagc ccgagcttct tggccgtggc
     3061 cgccgccagg ggcagcagga ggtccgcgtt caggttgaag cgggccagcg agccgaccgc
     3121 gaaggcgtcg cggtgccact tgcaccactt ggcggtggac tgcgcggtga cgtactcgtt
     3181 gacgacggac tcgaacttcc ggacgggcgt caggtccttg gtgtccgtgc tgccgatgcc
     3241 gccgtggtag aaggagtagt gcgtcgggtc gacgagcgcg acgtactcgg tgtcgcggtc
     3301 gaaggccggc agtttcggcg cgaggctcaa gacgacctcg gcgagggcct tgaggtccgg
     3361 gacgatgccc tcgagggtct tcttcagctc gcgcaggtac tgctcggtcg gcaggctggc
     3421 gaacccgccg ggcttgatgg tgacggggtg ggtcatgcgc ccgccgatcg cctccatcca
     3481 ctggttgccc gtgcggtgga gcttgatgac ggtcttgacg acgtccgggt ggctcttcac
     3541 gagcggcacg acggatttct ggccgagcag gtccggcgcg acgaggtagc cgacgtgcag
     3601 gctgtggctt tcgagctgct cggagtagtg gagcagcatg cggatcttgt cggtctgctc
     3661 ggagaccacg atgcccatgg cgtcctcgat ggccttgatg cacgagaacg agtgggtgac
     3721 ggagcagatg ccgcagatgc ggctgacgat ggtttggatg tcctcgaagc tgcggccgcg
     3781 gaccatggcc tcgaagaagc gaggcgcctc gggcacctgc cactcgagct tctcgatgcg
     3841 gccgtcggag gcgcgcagca cgatgttgcc gtgcccctcg acgcgggtga tgtggtggac
     3901 gtggatgtcg atggtcttgc tcatgatggg gcttccgcct tcctctactt cacgttctcg
     3961 gtggcttcct gcttgctgtt gaacagcagc agccggccct tcaagtcttc catggtcttg
     4021 ccgtaatgct ccatgacctc gcgcgcagcc tggacgttgg gatcgtccac gtagccgcgg
     4081 cagccgaagc accagaaccc cgcggagggg cacttggcgt cgcagccggc gcgggtgacg
     4141 gggccgaggc agatctcgtt gtattcgtag cggcacacgt tgccgcgcat cttgcactcg
     4201 acgcacaccg ggtagttcgg gattgccggc gtcttgccga gggccaggca gcgcaggatg
     4261 taggcgaatt ccttgttgtt gatcgggcag ccgtggatct tcaggtccac cttgaccacc
     4321 tcgtccagcg ccttggtcgg cgcggtgtcc aggtgcggca tcgagccgtc cttgccgtag
     4381 acctcccgcc ggacgtcctc catggtgaag ttgttcttca gcttgttcac gccgcccatc
     4441 gtcgcgcagg cgccgagggc gacgaggacc ttggcgcgcg agcggatctg gcgcagcttc
     4501 tcctcgtcgc cgggccgggt gatcgagccc tcgacgatgg cgatgtcgta ggtgtcgctc
     4561 tggtcgctga tggcctcccg ccactcgacg ggctggacca gcgacaggac gtcgaggagt
     4621 tcctcctcca ggttgacgat ctggagctgg cacccctcgc agcaggcgaa atcgaaaatg
     4681 gcgacgcgcg gcttgtccat cagatggcct cccgtacttc ggcgacgtcg gtgtagcgga
     4741 acaccggccc gtcctggcag acgtacaggc cgttgatctg gcagtgcccg cacttcccga
     4801 cgccgcactt catgtggcgc tcgagcgaga cgaagatccg gtccttcccg atgcccatct
     4861 tgtcgagttc gaggatcacg aacttgtaca tgatcggcgg gccgcagata acggcgaccg
     4921 tccgggccgg gtccaccttg accttcggca tgagcgtcgt aatgacgccc acgctcccct
     4981 tccagtgttc gtcgccgcgg tccacggtct cgaggaaggt cgtgtccggc tgggcggccc
     5041 actgcttcgc ctcctcgacg aacagccgct cggccgggct cttggtgccg aagaggatcg
     5101 tcacgcggcc gtagtccttg cggtgatgca ggacgtagtc gatcgccgag cggacgggca
     5161 ccaggccgat gccgccgcag acgaacagga ggtcctgccc cttcatcgcg ccgtccaccg
     5221 ggaactgcgt cccgaacggg ccgcggatgc cgaccttttt cccgggggcc aggcggtgca
     5281 tggcggcgga cacgttgccg acgttgcgca cgaccatctg gaatccgctc cggtacgtcg
     5341 gggacgagga gatcgagatc ggcgcctcgc cgatgcccgg gatcgcgacc tcggcgaact
     5401 ggccgggcat gtggcccagc ggccggccgc tggagagctt gaactcgaag aacatctcca
     5461 tcttcgtcat cggctcggcg cgcacgacat ccgccggctc gggcaggtag atgtcttttt
     5521 ccgccgtcag gatctggctc atcgcggtcc ctccgccagt cggttgtaga tttccaccgg
     5581 gttcgcgatc ttcgtcacgc aggcgccgat gcagcggccg cagccgacgc aggcgatctg
     5641 ccggtgctgc tcgaaaaggt actggccctt ccgcaggtag cggtggcggt agcgctccgg
     5701 tttcttcttc cggaagttgt ggccgccggc gacgagcgcg aactcggcga gcatgcagcc
     5761 atcccacgtg cggacgcgct cgccgctctg gaggtcccag cgcacgtcgt cgcggacgtt
     5821 gaagcagtag caggtcgggc acacgaggtt gcaggagccg caggagaagc agcgcttcga
     5881 cttctcctcc cagaccgggt gatcgtagga gcgggccagc agggcgggca ggtcccccgg
     5941 cttgaccttg agctcgtggc gccgcaggcg cttccggttg tcgtcccagg tctttttccg
     6001 gagctgcagg tcggccggag cggcgtccgg ctcgcccgcg agggatttca ccagcgcggc
     6061 gcccttgtcc gagcggctct cgacgaggta cgcgtcgccg atgcgggtca gcatcacgtc
     6121 gccgccctgc tcgaggaccg cggtgttcat gcagccggcg aacacgttgg ccgacggctg
     6181 ctgcacgtcc gaaacaacca gcgtcgcctg cgcgcggcgc ttgagatagt ggatgtccgc
     6241 ctggccctcg gcgaacacga cgtccatctg caggatcgcc gccgcgtcgt aggggtgcac
     6301 gccgaagagg acgaacggct gttcgtcgac cacgctctcg tagcccttcg ggttgaagcg
     6361 cagcaaggtc tcgcacggcg gctggaggaa ggccttgggc gcggtcacgg cgacgtcgta
     6421 gtccagccgc agcgcggagg tcttcgccag cggcgcgaag gcgaacttgt cgcccttggc
     6481 ctggacgccg atcaccgtct gccgcccgat caagccgtcc acccagcgct ccagcgcggc
     6541 gggccctatc ttcttgatgc tcatcaggtt ttctccttcg gcttcgcgcc ccgccgccgg
     6601 gcggcggatc attcgggaaa acgcgtgcaa atcagcgcga actgtaaaca aggcgcgggg
     6661 acatggctag ccttttttgc cgttttctta ttcggcggcc ggtgtggtaa acttgctcca
     6721 tgaaggggct cggcatggcg gtccgcggtt tcccggcgct ggccctgttg gcggtgacgg
     6781 gcggctgttc aggcgcttcg gagcatcccc cgcaggagga gggaatgacc gataaggccg
     6841 tcgggatggt cgaggtgctc aaggcctatg gcctccgcga tccggacgtg ctgcgcgcca
     6901 tgggaaaggt ccggcgtccc gagtacatcc cggagcggta ccgcgacggc tcggatccct
     6961 ggggcgatca tccctgttcc atcggccacg ggcagaccat ctcgcagccc ttcatcgtcg
     7021 cctacatgac cgagcgcatg gcgccgtgga aaggggagaa gttccttgag atcggcacgg
     7081 gctccggcta ccaggcggcg gtcctggccg aactgggggc cgaggtcttc accatcgaga
     7141 tcatccccga gctggccgag cacgcccgga gggccttggc ggcggagggc tatgcgcggg
     7201 tgaacgtccg cgccggcgac ggctaccggg gctggccgga acatgcgcct ttcgacgcca
     7261 tcctggtcac ctgcgccccc gaggagatcc cgcccgcgct ggtcgaccag ttgaaggatg
     7321 gcggccgcat gatcctgccg gtcggcgagg ccggcgcgca gcgcctggtg atcctgcgca
     7381 agaaagacgg gcgcgtcacg gtggaggacg atctgcctgt ccgcttcgtg cctatggtcc
     7441 gcggccggtg acccgatttc gcttgaagcc gcgggccggc tctggtttaa gagacccccg
     7501 gattacacag ccgggatcgg cccgcccgag acccgcctct ttcaacggag catgacatga
     7561 gcagcacgcc ggcaacggaa acggaatacc tcccccaggg cccgtccaac gtcctcgtca
     7621 tcaacgcggg tagttcctcg ctcaagttca ccatgtacgc catgcgcggc gagaccatcc
     7681 gcgccaaggg catcgtcgag cgcatcggcc tggccgatcc ctgcctcgtc tacgagcggt
     7741 tcgacggcaa gaagctcaag gagaaggtct cggtccggaa ccatgacgag gcgctgcggg
     7801 tgatctgcga caggctggtg gacccggcgg tcggcgtgct cgcgaacctg ggtgaggtcg
     7861 aggccatcgg gcaccgcgtc gtacatggcg gcgagaagtt ccgcgactcg gtccggatca
     7921 cggacgaggt caagcggatc atccaggaga tggccacgct ggccccgctg cacaacccgc
     7981 cgaacctcgg cggcatcgag gcctgcgccc atgtctttgg cgacatcccg aacgtggccg
     8041 tgttcgacac ggccttccac cagtccatgc agccttcgtc ctacctgtac gccatcccct
     8101 acgagtacta cgagaagttc ggcgtgcgga aatacggctt ccacggcacc tcgcacaagt
     8161 tcgtcgccca ggccaccgcc gcctacctgc gcaagccgat ggcccggctc aagctcatca
     8221 cgtgccacct cggcaacggc tgcagcatcg cggcgatcga ccgcggcaag gtgatcgaca
     8281 ccagcatggg catgaccccg ctcaacgggc tcgtcatggg cacccgctgc ggggacatcg
     8341 atcccgccgt cgtcctctac ctcgtgcgcc agggcatgag cgccgacgag gtggacagga
     8401 tgctcaacaa gcgcagcggc ctgctcggcg tcgccggcat cggcagcggc gacatgcgcg
     8461 acatcatcgc cgccgcggag agcgaaaacg accaggccaa gcgcgcgctc ttcatgttct
     8521 tccaccggct ggtgtcctac atcggcgcgt acgagacgat cctcgacggg gccgacgcgg
     8581 tggttttcac cggcggcatc ggcgagaaca gcgcctacgt ccgcgagcgc gtggtctccc
     8641 ggctcgggtg cctcggctgt ttcctggaca aggccgccaa cgaggccgcc gtcggccggc
     8701 aggccgtgat cagcacggcg gaaagcattc tgaaggcggt agttatgccc accaacgaag
     8761 agctgatgat tgcccgcgag accgtgcggc tgctttctcc cgccgcgtag caaaggagcc
     8821 gacgccatga gctttctcga tacgctcatt cccaaagccc gcgccgccgc ccgcacgctc
     8881 gtcctccccg agggccacga tccgcgggtc atggaggccg ccgccaagat cgcgaagcag
     8941 ggcattgccg cgcgcgtgat cgtcctggcc accccggccg agcgcgcgaa gacccgcgac
     9001 caggtctcgt tcgagggcct gccggtgcag gtgctcgact atctcaatgc ccccgagcgc
     9061 gagcggctga tgctcaagct cctcgagctc cggcgcgcca agggcatgac ccccgagcag
     9121 gcgcgcgata cgatccagaa ccgcctctac ttcggcaaca tgatggtccg cgagggcctg
     9181 gccgacggca tggtcgccgg ctccatcgcc tcgacgccgg acatggtccg cacggccttc
     9241 cagtgcgtcg gcaccgcccc cggcatcaag ttcggcagca gctgtttcat catggacctt
     9301 gcgaggccca cgcccggcgg gcagggcgtg ctgctctacg ccgactccgg cgtcaatccc
     9361 aacccgaccg ccgagcagct cgtggacatc accgtcgcca cgatccgcac ctgccaggcc
     9421 ctggtcggcg gccaggcccg cgtcgccatg ctgtccttct ccacccgcgg cagcgcgaag
     9481 catgacctcg tggacaagat ggttcgcgcg actgacctgg cgaaaaagcg tgtcgccgag
     9541 ctcgggctcg atgccctcgt ggacggcgag ctccaggcgg acgccgcgat cgtcccggcc
     9601 gtcgccgcct caaagtgcgc ggacagcccg ctcaagggcg cggcgaacat cctgatcttt
     9661 cccgacctcc aggccggcaa catcagctac aagctcacgg agcgcctcgc cggggcgacg
     9721 gcctacggcc cgatcatgca gggcctcgcc aagcccgtga acgacctgtc gcgcggctgc
     9781 agcgcggacg acatcgtcgg cgtcgccgcc atcaccgtct gccaggcgat cggctaggtt
     9841 gattccgaag cgcggtagcc ggcggcccca gacgcgcagg tgttgcccgc cgggtcgcgg
     9901 cgacgcctga aaacgtgtgt atcacagttt ataaactgtg atatattgat atgttcaaac
     9961 gcgggaatat ataggaaatt gcgctctggg gcctgtgtat cacagtttat aaactgtgat
    10021 acatggcctg ggctcgaggc ggttttttcc ctctcctggc gcgtgtctgc cccggtcgcc
    10081 tcgcctccct cgctgccctc tgccgcgcgg cgggccgcgt ccgtatgtat caggtccccg
    10141 ggcacgcctt cgccgcggag ggcaggaggt acaggctcgg gttgcctccg gtcttgcaag
    10201 cgccgcgccg ctggtctacg ttgcctcgtc atggatgccg cgatcataac cacttaccgg
    10261 tgcccgaacc gctgccacat gtgcaacgtg tggaagttcc cgacgaaggt cgaggaggag
    10321 ttcaagcccg gcctgctgga gaagctaccc cgcctccgct tcgctaatgt caccggaggc
    10381 gagccgaccg ttcgcgagga cctggacgaa atcctggccg tgctgcgacg caaggccgag
    10441 cgggtcgtca tcagcaccaa cggattcctc accgagcgcc tcctcgggct cgcggaacga
    10501 caccccggga tcggcatccg catcagcatc gagggcctgc ccgcggccaa cgacgaactg
    10561 cgcggcgtga aggacggctt cgaccacggc ctgcgcacgc tgctcgaact ccagcggcgc
    10621 ggcgtgaagg acatcggatt cggcatcacg gtctccgacc gcaacgcgaa ggacatgctc
    10681 gaactctacc agctcgccaa gtccatggac gtggaattcg cgacggccgc cgtgcacaac
    10741 tcctactact tccacaagta cgataaccgg atcgagaagc aggacgaggt gatcgcctgt
    10801 ttccgggaac tggtcaggga actgctccga acccgcaaaa tcaaaaactg gtaccgcgcc
    10861 tacttcaacc atggcctcat ccagttcgtc cggggccagc cgcgcctgct cccctgcgag
    10921 gcgggcacgg agaatttctt cctcgatccg tggggcgaaa tacggccgtg caacggcatg
    10981 gaggaaggcg tctggtgcga gagcatgggc aacctgcacg cgcagacctt cgaggggatc
    11041 tggaacgggc cgaaggccgc cgccgtgcgg gagaaggtga agcattgccc caagaactgc
    11101 tggatgatcg gcaccgcatc gcccgtgatg aaaaaataca tccgggttcc cacgctctgg
    11161 gtcctgcgca acaagtggcg cgtcctgacc ggccgcgagc cctgcacgga ttgagcctgg
    11221 gtcatggacg gctccattgc ctatcccgcc ggccttctgg tggatctgcc cctggaaggc
    11281 ccgccggtcc tggcctgcgg cgcggacggc aaggccgcgc cctgcgccgc gcagggcggc
    11341 cgcgccttcc tcgcggaacc cgtcggtaat cttgccgagt acgaagccgc cgcgcggtac
    11401 cgggcgaccg tcgaggccct gctggatcgg ctcggtatca aggcttcggt tgtcgcgcac
    11461 gacctgcatc cggactatga atccaccctc ttcgcccggg cgtttcccgc ggatcgccgg
    11521 atcgccgtgc agcatcacca cgcgcacgcc gccgcgtgca tggccgagca cgggctgacc
    11581 ggtccagtga tcggcgtcgc gctggacggc tcgggctacg ggccggacgg cacggtctgg
    11641 ggcggcgagt tcctcgtcgc ggattacgcg ggcttccgcc gcgccggaca tttcaaaccc
    11701 tatcgcctgc ccggcggcga gggcgcgatc cgtaacccgc cgcgcatggc cttgagctgg
    11761 ctggcggcgg agtgcggaga agcggcggat gccctcgccg cgcggtggct gcccgcgatt
    11821 cccggggagc agcgaacgat gctgctcggc ctggtcgcct ccggcgcgca ttcgccgtgg
    11881 acttccagcg ccggccgcct tttcgacgcg gcctcggcgc tgctcggcct gtgcgaccgg
    11941 atcgcacacg agggcgatgc cgccatccgg ctccaggcgg ccgccgaccc gcgcgagacc
    12001 ggcgaatggc cgttcgcgct ggaagaggcc gtcctgtcct tcggtccggc cctccatgcg
    12061 ctgctggaac agcgtgccgc gggcgtcgct atccccgtgc tcgccgcgcg tttccaccgg
    12121 accgtcgccg ccggcgcggc ggccctgtgc gggcggttgc gcgagcgcga gaaaccgaac
    12181 cgcgtcgtcc tttccggcgg cgtcttccag aacgatctcc tccgcgcgtg gctgacggag
    12241 actttgtccg cctcgggttt tgaagtatat tgtccgcggc gggttccgcc cggcgacgcg
    12301 ggcgtggccc tgggccaggc cgcggtggcg tcggcgtcgg gctggtagca tctaactcca
    12361 tg
//
LOCUS       DAOOTK010000100        12329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3425_length_12329_cov_5.960730, whole genome shotgun sequence.
ACCESSION   DAOOTK010000100 DAOOTK010000000
VERSION     DAOOTK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12329
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3425_length_12329_cov_5.960730"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="PK634_10750"
     CDS             complement(<1..2073)
                     /locus_tag="PK634_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOW98255.1"
                     /translation="MRGSLQAVVILAALALAAPWDAARAATEPGDANDDGIVDMEDVR
                     HVLQYLVHEVPLAETNAADASQDGKIDVDDAFIIARHLGGESLIVYVDTPFGGTRVFE
                     AGSMLVVQAFESFFPAAATNGTIRIRSESAGYDSGVQPMSLVAGQRSYLFHWDTLAAL
                     PAADYVVRVTIGDDEAEQVVALAHPSFGPPSIADAVDAQCPGAGFPLQLRRFIGDGHN
                     SYPLLGPFGRSWKPDFAVKLDEFADGTVLFHGRAPFHRYYRSRPDGSYESPVGDYSTL
                     TFTGGCFRLEEKSGLAYQFRDDLQALFMEDRNGNRLTFGYDEQGRVTNVLHSSGDALT
                     VRYNSQGRITNLVDHVGRVTLYEYDAGGQHLVRVTGPGGDTTTCSYYTNAHYYINDRL
                     KSVTFADGTEAHYEYDEQGRVVRTTGKDGVNAVTFAYEADGVTRIRDVFSNETIAAYT
                     VRRQPVLFTDALGHSISNRYDALFNRIEEWDAFGRPTRYAYDANGNMTNMVDAAGHAF
                     AFRYDADTHKLLELRDSRGALSRFVYDALGNLLKAVFADGTEETYVYNARGLPTSKTT
                     RSGAVMSYAYDGRGVLLSRTYPGGARDMYEYDAAGRLAQASNTIWAISNVYDEADRLV
                     RVTYPGGRSFVYAYDGGDRQAGMTDPDGRNLGYAYDRFGFLSAIREDATGALVAAYHP
                     IPLATIQQL"
     gene            complement(2202..3362)
                     /locus_tag="PK634_10755"
     CDS             complement(2202..3362)
                     /locus_tag="PK634_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98256.1"
                     /translation="MNGVRVIAWGGAALLMGATTALFVWDVFGLRTPPRNPPVALPGQ
                     PLAASSVALDLEVEIVNPTSHSIAYFYTWNAGRVFGRVIDSQSVPGRKYPVRVRLSPE
                     GLVLFDDSWGLEFAGPLQSGPLSVGVTFERHSGDVERVSCECRYEAWVSPDNVALADP
                     GQPWTFSHGAWSTTGGTALVVHGVRSYERDLGQRAAVIGRFTAPRMAADVKTALASAL
                     PDVPATAVHVLPVYEEGRFTDDVGRKATVFELGYSVPRAMAPLPAPRPLQRLKTAVVA
                     AFPEALSSSDLRDWNPSGLRVAQPIQRVPFRVASDAPVVRAQLARWLRDASAGGRVAD
                     PLLWPAEPGGARALRPLRDYRMSIVVAGGTWEPALEALAQRIAARAEAGEEP"
     gene            complement(3391..5223)
                     /locus_tag="PK634_10760"
     CDS             complement(3391..5223)
                     /locus_tag="PK634_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98257.1"
                     /translation="MKRLAETSGRGMDGPRRRRAIGAARALFLPWLISWLAGARAGRA
                     ETFIISLPVGGAIPIELSHVGPEGGLVSPGEPSVPPFRPPSIFSAPLPSGSGARALGL
                     GAFTAVADDATAASWNPGGLVQLERPEASFVLRYSAHESRHASDDADYQVGRDEYEGQ
                     NLNYLSAVLPVKAFNRPCVVSLNYQEAYDFEMRFHADQLGRSSRREEESRQSATSDTQ
                     VDHFSDGVLDVTITSELTTRLTSQMRQLLQSDMLTDISFEQEGIIDAVSPALAVEVIP
                     GLSVGGALNIYQDDLFGNRPIGSSSRASYSGASSSRVQTRDTRETTGTYTYEGTWTPA
                     PIEIMPGIVVPIPPVPISGEGVYPPFSDSGSGGGSDGIVFDGEYSEENRFDDLHGWNL
                     TLGGLWSVSRHLSLGGSVDLPWLAEAEQEQTIRSRETAYDASRTVVLDRSEYVVTERK
                     DVEFRFPLFWSVGAVWKWNNVLSTALDVSQTRWSDFYYRADGEEKINPLDGRAYEANR
                     LDDCWAVRLGAEYIWVLRATEIPLRAGLSWEQRPAVGAPDEFYGVNAGTGLSLGKDPG
                     KLILDVAYAYSWGDDVLGTLVPEQEGLSTDMREHQVYVSCIYHF"
     gene            complement(5235..7268)
                     /locus_tag="PK634_10765"
     CDS             complement(5235..7268)
                     /locus_tag="PK634_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98258.1"
                     /translation="MNRPRNHIGLAAGAALGLILFSCGHLESPPEAGRETTFYHNWWN
                     CYERGLELMRAGNYEGASLEFERTLGLKPGARFGFAREMWRARTYGLHFVEDYFPHRE
                     LGVCRYRLGQDEEAIPLLVRSLEQEPSSRAKFFLNLARSRQARKHPASPPQITIHGPS
                     QERPVRDREVRLSGLAAAEARIGRVAVNGQPVFVELAEKEIAFDQVVPLREGSNVIAC
                     LAEDLAGQVAATNVTVWSDRTPPGVVIAGISESNSGRFVRGTASDNYGLAYLAIDGTV
                     LVKTVPGSEAPVSREFVVPMSSRDALAIEAADVAGNTLRSVVPLGEGDDVFRRLPPRY
                     ASAGWIASAADGTEPEEPAPEDRMRPSLTLHISAPEVAVYDEEYYLEGLASDPGGLAA
                     LSINGESLMTGPEKGGVQKLFARRLALDVGTNRFVLAARDRAGNKTEKEVTVIRLQPD
                     YLDQKHRLRVALAPCSGGGDTAEAVGAYMEAAVLRSPARFAMVERDDRAWARIVQEQE
                     ISLSDLADCSAALRIGKMMPAELLLLSSVIRHERGVTVFARLVDTSEGTRAWAGDIYC
                     DEPERALEEQVDALALKLKQAFPLLDATVIRIDGERITMDAGTERGVRAGTRLLVLKT
                     GAGGDRRQGQVLYHESQPAEIEVTRTGGKDCLGLVKPRAAAELIDPGDAVYTR"
     gene            complement(7308..10061)
                     /locus_tag="PK634_10770"
     CDS             complement(7308..10061)
                     /locus_tag="PK634_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW98259.1"
                     /translation="MRTYLRFPGDRSSYVLDNGIGHHDQPGRRPPTWPPGSRKDYHMK
                     RLTWLGAVALMALLPLARAQTPGQDNTVSVAAMDQGGTVPAASALTSAEDLYLSESDY
                     YLVRGQRIPLRRDAQRMAVKLTKQAREAYQSEMGRQPRGATSAHSAALRDALGFSDAE
                     ADVSLEQEVAEKGLVIVEVRPKSGHALGRTRLRDAGRAKGVEYANPVFAPAVGLGQIL
                     VTDEILVCFASDYDDAKVALFAAEHGLASVRRLRGRSNTWLLRLDDPRSQTALDVANT
                     LHGQPGVLWAQPSFEMEIRLQSNDPYYASQWHLHNTGQSGGLAGTDVRAPEAWALGVT
                     GSNIVIAIVDSGVDLSHEDLVIWQNPGESGSGRESNGIDDDGNGYVDDYRGWDFYSMD
                     NNPSPGGSHGTACAGVAAARGNNAKGVAGVACGAAVLPIKITSTDTGFANDAAVHDAI
                     VYAADHADILSCSWTYSLSESIMDAIDYAVTRGRGGRGSPVFVAAGNDAARGWTTFGL
                     TGAPAGTFVVGWEYRTDDSPTGALNDAWLDNVTLQDGSVERFSGTTPPTLPSGWSGAG
                     TMPWTTVSDAEHAIGDSRSARAGQIGPDGWSRLYVQKNYSVPGNYSFSCWVGPAAGQT
                     LYALYYYSSRWIAYKCYYGAYVGFPASYSNSIAVGACGNLGAHSSYSQYGPELDLVAP
                     SGGGTLGIVTTDRTGANGYSTTAYCTNFSGTSAATPAAAGVAALVLAANPQLTAAEVK
                     RVLQETAVDMEQPGFDIYTGRGRVNAYAAVMASAANNPPVIESRAPTNALVFVTPGAS
                     ARFAIGARDPEGTALTYRWYLDGALQASTATNCVVATSSGMVGPHQVRAEVSDRGGNT
                     VQVSWQLLVGEGDPNLVGYWPFDDKTGRDQSGWGNDLSIQGAAGFNWDGIKGPDLDLD
                     VQP"
     gene            complement(10071..11093)
                     /locus_tag="PK634_10775"
     CDS             complement(10071..11093)
                     /locus_tag="PK634_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOW98260.1"
                     /translation="MITREAPAPRYRGAELEALGPQATLAAGPANVAVTASLLSPQPT
                     QTFFLSAWIRCNRLPTATAGFAGIAGKYQAGNTNASEWLLGVNSAGKLFLESTGAGSP
                     KQVVAASNIKTGWFHHVAAQWETNGTATLWMDGRLESSAVLARPRGGSLPMILGDTYP
                     PDGSHALKGRIDEVRVWVKKADTATVQALLAAAPDLDKDGLIDGNDPDGNNDGVDDAW
                     AARYFGDRYSGDPNVDLDKDSFVNRDESVAGTDPTNPYSYFRAWDMRPGGGQWKTAGN
                     KLEFEVDGVAGRVYQVWTRSRLITTQAWVAASSPVPCAQTGPFVVSVAANPTNQVFYR
                     VTVTKP"
     gene            complement(11310..>12329)
                     /locus_tag="PK634_10780"
     CDS             complement(11310..>12329)
                     /locus_tag="PK634_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOW98261.1"
                     /translation="SHAGGEFVFVRQGASIPEEAVAVPAAGQSVRGPEAAAAPSSREL
                     LRDAALLSQQGATGAAHRLLAMTPAGSSNAALLEAVADYLDREREQNSREELRGLIRQ
                     LEERKHNAQPADLGAARPRIVACMGPDSLRGGSDDEADALLYRISLRAALEDDGRVRV
                     VERDALETVLQEMDLGASELAEPDARTAIGKVLPASYLLLGTLLSRGDSQQLLMRLVD
                     TETTLALGSFSQAVGPQEDVMAAAGRIAGAMITRLIEQVPLTACVESVEKGRLRAAVG
                     RLHGARAGVQFQLLRRTTAEQESVAGMAAITELGEKQSVLTMMSGEVPAEWAPGELWL
                     EELAP"
BASE COUNT         2032 a         4270 c         3975 g         2052 t
ORIGIN      
        1 caattgctgg atcgtggcca gggggatggg atgataggcc gccaccaggg cgcctgttgc
       61 gtcttcgcga atggcgctca agaatccgaa gcggtcatag gcatacccga gattcctgcc
      121 gtcgggatcc gtcatgcccg cttgccggtc cccgccgtca tacgcgtaga cgaacgaccg
      181 cccccccgga taggtcacgc gcacaaggcg gtcggcctcg tcgtacacgt tcgagatcgc
      241 ccaaatcgtg ttggaagctt gcgccagccg tccggccgca tcgtactcat acatgtcgcg
      301 cgcgccgccg ggataggtcc tggagagaag gacgccgcgc ccatcatacg cgtaggacat
      361 gaccgctccc gagcgggtgg tcttggacgt cggcaatccc cgggcattgt agacgtacgt
      421 ctcctcggtt ccgtcggcga agacggcctt cagaagattc ccgagggcgt cgtagacgaa
      481 gcgcgacagc gcgccgcggc tgtcccgcag ttccagcagc ttgtgggtgt ccgcgtcgta
      541 gcggaacgcg aaggcgtgtc ccgccgcgtc caccatgttc gtcatgttcc cgttcgcgtc
      601 ataggcgtaa cgggtggggc ggccgaaagc atcccactcc tcgatgcggt tgaacagggc
      661 gtcgtagcgg ttcgaaatcg agtggcccag cgcatcggtg aaaaggaccg gctggcgccg
      721 gacggtatag gccgcgatcg tttcgttcga aaaaacgtcg cggatgcgcg tgacgccgtc
      781 cgcctcgtac gcaaacgtta cggcattgac gccatccttt cccgtggtcc ggacaacccg
      841 gccctgctcg tcgtactcgt agtgcgcctc cgtgccgtcc gcgaacgtca cggacttcaa
      901 ccggtcgttg atgtagtagt gggcgttcgt gtagtaggag caggttgtcg tgtcgccgcc
      961 cgggccggtc acccggacga gatgctggcc gccggcgtcg tactcgtaaa gggtcacgcg
     1021 ccccacgtgg tccaccaggt tggtgatccg gccctggctg ttgtagcgca cggtcagggc
     1081 gtcgccggag gaatgaagca cgttggtgac ccgcccctgc tcgtcgtaac cgaacgtcag
     1141 ccggttcccg ttccggtctt ccatgaaaag cgcctgcagg tcgtccctga actggtaggc
     1201 gaggcccgac ttctcctcga gccggaaaca gccgccggtg aaggtcagcg tcgagtagtc
     1261 tcccacgggg gattcgtagg atccgtccgg ccggctccga tagtaccgat ggaagggcgc
     1321 gcggccgtgg aagagcaccg tgccgtcggc gaactcatcg agcttcaccg cgaaatccgg
     1381 cttccaggaa cgcccgaacg gccccagcag gggatagctg ttatgcccgt cgccgatgaa
     1441 gcgccggagc tgcagcggga atccggcgcc cgggcactgc gcatccaccg cgtcggcaat
     1501 cgacggaggc ccgaagctgg gatgcgccag cgccacgacc tgctccgcct catcgtctcc
     1561 gatcgtcacg cggacgacgt aatcggcggc cgggagcgcg gccagcgtgt cccagtggaa
     1621 gagatacgac cgctggccgg ccacgaggga catgggctga actccggagt catagccggc
     1681 gctctccgac cgtatgcgga tcgtcccgtt ggtcgcggcc gccgggaaga aggactcgaa
     1741 cgcctggacg acaagcatgc tgcccgcttc aaagacacga gtccccccga acggggtgtc
     1801 cacgtaaacg ataagcgatt ccccgcccag gtgccgggcg atgatgaagg cgtcgtcgac
     1861 gtcgatcttt ccgtcctggg aggcgtcggc agcattggtt tcggcaagcg ggacctcgtg
     1921 aaccaagtac tggaggacgt ggcgcacgtc ctccatgtcg actattccat cgtcgttggc
     1981 atcgcccggc tccgtcgccg ccctggcggc gtcccatggc gcggccagcg ccagggcggc
     2041 aaggatcacc acggcttgga ggctccccct catgtttttg ctgctcatca cgcggacgcc
     2101 gacgtcaacc actccttctg cctgccattt accagaagaa agccctgaat gaaagcccgc
     2161 gccgcagccg aaatggccgc cggccgtcct gtcgcgccgg atcacggctc ctccccggcc
     2221 tcggcgcgcg cggcgatccg ctgcgccagg gcctccaagg ccggttccca ggtcccgccg
     2281 gcgaccacga tggacatccg gtagtcgcgc aaggggcgca gcgcgcgggc gccgccgggt
     2341 tccgcaggcc agaggagagg gtccgccacg cgcccccccg ccgaggcgtc ccggagccat
     2401 cgcgcgagct gcgcgcgcac gaccggcgcg tcggaagcca cccggaaggg cacgcgctgg
     2461 atgggttgag cgacgcggag tccggaaggg ttccaatcgc gcaggtcgct actcgacagc
     2521 gcctccggga atgccgcgac caccgccgtc ttcaggcgct gaagaggccg gggggccggc
     2581 aacggcgcca tggcccgagg cacggaataa cccagttcaa agacggtggc tttgcggccg
     2641 acgtcgtccg tgaaccgccc ctcttcgtac accggcagga cgtggacggc cgtggcgggc
     2701 acgtccggga gggcggacgc cagcgctgtt ttcacatccg cggccatccg gggcgcggtg
     2761 aatcggccga tgacggccgc ccgttggccc aggtcccgct catagctcct gaccccgtga
     2821 acgacgagcg ccgtgccgcc cgtcgtcgac cacgcgccgt ggctgaatgt ccacggctgg
     2881 cccggatcgg cgagcgcaac gttatccggg ctgacccacg cttcgtagcg gcattcgcac
     2941 gagaccctct cgacgtcacc ggaatgccgc tcgaatgtga cgcccaccga cagggggccc
     3001 gactggaggg gcccggcaaa ctccaaaccc caggagtcat cgaagagcac cagcccctcg
     3061 ggcgaaaggc ggacgcgcac cgggtatttc cggcccggca cggactgcga atcgataacc
     3121 cgtccaaaca cacgcccggc gttccaggtg taaaagtaag cgatggaatg ggaagtcgga
     3181 ttgacgattt ccacttcgag gtcaagcgcc acggagctgg ccgcgagcgg ttgcccgggc
     3241 aaggcgaccg gcgggttccg cggaggcgtc cggaggccga atacatccca cacaaaaagg
     3301 gctgtcgtgg cgcccatgag cagcgccgcc ccgccccagg ctatgacgcg caccccgttc
     3361 atctcttttc ctggcccagc gttcttcttt tcagaaatgg tatatgcacg acacgtacac
     3421 ctgatgttcg cgcatatcgg tgctcaatcc ttcttgctcg ggcaccagcg tgccgagcac
     3481 gtcgtcgccc caggaatagg cataggccac gtcgaggatc agcttgcccg gatccttgcc
     3541 cagcgacagg cccgtccccg cgttgactcc gtagaattcg tccggcgcgc cgaccgccgg
     3601 gcgctgctcc cacgagaggc ccgcccggag cgggatctcg gtggcgcgca ggacccagat
     3661 gtattcggcg cccaggcgga cggcccagca gtcgtccagc cggttggcct cataggcgcg
     3721 gccgtcgagc ggattgatct tctcctcgcc gtccgcccgg tagtagaagt ccgaccagcg
     3781 ggtctggctc acgtccaggg cggtggaaag cacgttgttc cacttccaca ccgcgccgac
     3841 tgaccagaac agcgggaacc ggaattccac gtccttccgc tccgtgacga catattccga
     3901 gcgatcgagc accacggtcc tggaggcatc gtacgcggtc tcccggctcc ggatggtttg
     3961 ttcctgctcc gcttccgcca gccaggggag atccacggag ccgcccaggc tcaagtgccg
     4021 ggagacactc cacaggccgc ccagggtcag gttccagccg tggaggtcgt caaagcggtt
     4081 ctcctcgctg tattcgccgt cgaagacgat gccatccgaa ccgcctccgc tgccgctgtc
     4141 cgagaagggc gggtaaacgc cttcccccga aatgggcaca ggcggaatgg gcaccacgat
     4201 cccgggcatg atctctatcg gggcgggggt ccaggtgcct tcgtaggtat aggtccccgt
     4261 cgtttcgcgg gtgtcccgcg tctgcacgcg actgctggac gccccggaat acgaagcccg
     4321 gctggaggag ccaatgggcc ggttcccgaa cagatcgtcc tggtagatat tcagggcccc
     4381 gcccaccgaa agcccgggga tcacctcgac ggccagggcg ggagagacgg catcgatgat
     4441 cccctcctgc tcaaacgaga tatcggtgag catgtcggac tgcaggagct gccgcatctg
     4501 gctggtcagg cgggtcgtca gctcgctggt gatcgtcacg tccagcacgc cgtccgaaaa
     4561 gtgatccacc tgggtgtcgg aagtggcgct ttgcctcgac tcctcctcgc ggcgggaact
     4621 gcgccccagc tggtcggcat ggaaacgcat ctcgaaatcg tacgcctcct ggtagttgag
     4681 cgacacgacg caagggcggt tgaatgcctt caccggcagg accgcgctca agtagttgag
     4741 gttctggccc tcgtattcgt cgcggcccac ctggtagtcc gcgtcgtccg aggcgtggcg
     4801 gctctcgtgg gcggagtagc gcagcacgaa ggacgcctcg ggtcgctcca gttgcacgag
     4861 gcccccgggg ttccaggaag ccgccgtggc atcgtcggcc acggccgtga aggcccccag
     4921 ccccagggcc cgggcgccgg acccgctggg cagcggggcc gagaagatgc tgggcggccg
     4981 gaagggcggc acgctgggtt cccccggcga gacgagcccg ccttccgggc cgacgtggct
     5041 caactcgatc gggatcgccc cgcccacggg caggctgatg atgaacgtct ccgcccgccc
     5101 cgcccgcgcg cccgccagcc atgagatcag ccacggcagg aaaagcgcgc gggcagcccc
     5161 gatcgcgcgc cgccgccgcg gaccgtccat ccccctgccg ctcgtctccg cgagtctttt
     5221 cacggtcgat tccctcatcg cgtgtaaacc gcatcgcccg gatcaatgag ttccgccgcc
     5281 gcgcgaggct tgacgagacc caggcaatcc tttccgcccg ttcgcgtcac ctcgatttca
     5341 gcaggctggc tctcgtgata gagaacttgg ccctgccttc tgtcgccacc ggcccctgtc
     5401 ttgaggacca gcaggcgcgt ccccgcgcgc accccccgtt ctgtcccggc gtccatggtg
     5461 atgcgctccc cgtcgatccg gatcacggtg gcgtccagga ggggaaacgc ctgcttgagc
     5521 ttcagggcca gggcgtccac ctgttcctcg agggcgcgct cgggttcatc gcaatagata
     5581 tccccggccc acgcgcgggt gccctccgag gtgtccacca gccgggcgaa cacggtcacg
     5641 ccgcgctcgt gccgaatgac ggacgacagc agcagcagtt cggccggcat cattttgccg
     5701 atgcgcagcg cggcggagca gtccgcgaga tccgaaaggc tgatctcctg ctcctgcacg
     5761 atgcgcgccc aggcccggtc atcccgctcc accatggcga accgggcagg tgagcgcagg
     5821 acggccgctt ccatataggc gccgacggcc tccgccgtgt cgccgccccc gctgcacggc
     5881 gccaacgcca cccgcagccg gtgtttctga tcaagataat caggctgcaa gcggatgacg
     5941 gtcacttctt tttccgtctt gttgccggcg cgatccctgg cggccaggac gaaccggttg
     6001 gtccccacat ccagcgccag ccgacgggca aacagtttct gcacgccgcc tttctccggg
     6061 ccggtcatca ggctctcgcc gttgatgctc aatgccgcca gccctcccgg gtcggacgcc
     6121 aggccctcga ggtagtattc ctcgtcgtag accgccacct cgggcgcgga gatgtgcagc
     6181 gtcaaggaag gcctcatgcg atcttcggga gccggctcct ccggctccgt cccgtcggcg
     6241 gctgaagcga tccaccccgc ggaggcatac cgcgggggga ggcggcggaa gacatcgtcc
     6301 ccctccccca gcggcacaac acttcgcaac gtgttgccgg ccacgtcggc ggcttcgatg
     6361 gccagggcgt cccgggatga catcggcacc acaaactccc gggaaaccgg cgcttccgat
     6421 ccggggacgg ttttcacaag gaccgtccca tcgatggcca ggtacgccaa cccgtaattg
     6481 tcggaggccg tgccgcggac gaatcgaccg ctgttgcttt cggaaatgcc ggcgatgacg
     6541 acgccgggcg gggtccggtc cgaccagacc gtgacgttcg tggcggcgac ctgcccggcg
     6601 aggtcctcgg ccaggcaggc gatcacgttg ctcccttcgc gaaggggcac cacttgatcg
     6661 aacgcgatct ctttctcggc cagctccacg aaaacgggct ggccgttgac cgctacccgg
     6721 ccgatcctgg cttcggcggc cgccagcccc gaaagccgca cctcgcgatc ccgcacgggc
     6781 ctttcctgcg atgggccgtg aatggttatc tggggggggg acgcggggtg tttcctggcc
     6841 tgccgcgacc gggccaggtt caggaagaac ttggcccggc tggacggctc ctgctcgagg
     6901 gaccggacca gtaacgggat ggcctcctcg tcctggccca accggtaccg gcacaccccc
     6961 aactcccggt gcggaaaata atcttccacg aaatgcaggc cgtaggtccg ggcccgccac
     7021 atttcgcggg cgaaaccgaa ccgggccccg ggtttcaggc ccagcgtccg ctcgaattcc
     7081 agcgacgcgc cctcgtagtt ccccgcccgc atgagttcca gtccgcgctc atagcagttc
     7141 caccagttgt gatagaacgt tgtttcccgg cccgcttccg gaggactttc caaatgtccg
     7201 cacgaaaaca ggatcaggcc aagggccgcc cccgctgcca gcccgatatg gttccgaggt
     7261 ctattcaagc ctcccccgcg cgtttccctg tctctatgcc gccgaggcta cggctgcaca
     7321 tcgaggtcca ggtccggacc cttgatgcca tcccagttaa agcccgccgc gccctggatg
     7381 gagagatcgt tgccccagcc ggactggtcc cgcccggttt tgtcatcaaa cggccagtat
     7441 cccaccaggt tgggatcgcc ctcgccgacc agcaattgcc aggagacctg aacggtattc
     7501 ccgccccggt cgctcacctc ggcgcgtacc tggtggggcc cgaccatacc tgaactcgtc
     7561 gccacgacgc agttggtcgc ggtggaagcc tggagcgcgc cgtccaggta ccatcgatat
     7621 gtcagggccg ttccctcggg atcgcgggcg ccgatcgcga accgcgccga ggcgcccggg
     7681 gtcacgaaga caagcgcatt cgtcggggcc cgggattcga tgacgggcgg gttgttggcg
     7741 gcggaggcca tcacggcggc ataggcgttg acccggccgc gtcccgtata gatgtcgaag
     7801 ccaggctgtt ccatatcgac ggccgtctcc tgaagcaccc gcttgacctc ggccgcagtc
     7861 aactgcggat tggccgcaag gaccagggcg gccacgccgg cggccgcggg cgtagcggcc
     7921 gatgtgccgg aaaagttggt gcagtacgct gttgtcgagt acccgttggc gccggtgcga
     7981 tcggtggtga cgatgcccag cgtgccgccg ccgctcgggg ccacgagatc gagctccggc
     8041 ccgtactggc tatagctgga gtgagctccg agattgccgc aggcgcccac ggcaatgctg
     8101 ttggagtagc tggcaggaaa gccgacgtat gcgccgtagt agcacttgta ggcgatccag
     8161 cgcgagctgt aataataaag cgcgtagaga gtctggcccg cggccggacc aacccagcag
     8221 gagaaactgt agttgccggg aacggagtaa ttcttttgca cgtacaagcg cgaccagccg
     8281 tccgggccga tctgccccgc ccgcgcggag cggctgtcgc cgatggcgtg ttcggcgtcg
     8341 ctgaccgtcg tccacggcat cgtgccggcg ccgctccagc cggatgggag cgtcggcggg
     8401 gtagtgccgg agaaccgctc taccgaacca tcctgcaatg tcacattgtc cagccaggcg
     8461 tcgttgaggg cgcccgtggg actgtcgtct gtccgatact cccagcccac gacaaatgtg
     8521 ccggccggcg cgccggttag gccgaaggtc gtccatccgc gggcggcgtc gttccccgcg
     8581 gcgacgaaca cgggagagcc gcgccccccg cgaccccgcg tgacggcata gtcaatcgca
     8641 tccatgatgc tctcggacag gctgtaggtc caactgcaac tgagaatatc cgcgtggtcc
     8701 gccgcgtaca cgatcgcgtc atgaacggcc gcgtcattgg cgaagcccgt gtccgtggac
     8761 gtgatcttga tcggcagcac cgcggctccg caggccacgc ccgcaacgcc tttggcgttg
     8821 ttgccgcgtg cggccgcgac ccccgcgcat gcggttccgt ggctgccccc gggggaaggg
     8881 ttgttgtcca tgctgtagaa gtcccagcca cggtagtcgt ccacgtagcc gtttccgtca
     8941 tcgtcgatgc cattactctc gcgcccgctg ccggactcgc cggggttctg ccagatcacg
     9001 aggtcctcgt gcgacaggtc cacgccggaa tccacaatcg cgatcacgat gttagagccc
     9061 gtgacgccca gggcccacgc ctccggcgca cgcacgtcgg tccctgcaag gccaccgctt
     9121 tggcccgtgt tgtgcaggtg ccattgtgaa gcatagtacg ggtcgttgct ttgaagccgg
     9181 atctccatct cgaaactcgg ctgcgcccac agcacccctg gttgaccatg gagcgtattg
     9241 gccacatcga gggccgtctg cgaacggggg tcgtccaagc ggagcagcca cgtattgctt
     9301 ctgccgcgca ggcgccggac cgatgcgagg ccatgttccg cggcgaaaag cgcaaccttc
     9361 gcgtcgtcat aatcgctcgc gaaacagaca agaatctcat cggtgaccag gatctggccg
     9421 agcccgaccg caggcgcaaa caccgggttg gcgtactcca cacccttggc cctgccggca
     9481 tcgcgcaggc gggtccggcc gagggcatgg ccggacttcg ggcgcacttc cacgatcacg
     9541 aggccctttt cggccacctc ctgctccaga gacacatcgg cctccgcgtc ggagaagccc
     9601 agggcatcgc gcaaagccgc gctgtgcgcg gacgtcgcgc ctcgtggctg ccggcccatc
     9661 tctgactgat aggcctcgcg cgcctgcttc gtcagcttga cggccatgcg ctgggcatcg
     9721 cggcgcagag ggattctctg gcctcgaacc aagtagtagt cggattcgga caggtagagg
     9781 tcttcggccg acgtcaaggc tgatgccgcc ggcacggttc cgccttggtc catcgcagcg
     9841 acagagaccg tgttgtcctg gcccggtgtc tgtgcccggg caaggggaag cagggccatc
     9901 agggcgacgg cgcccagcca ggtcaggcgt ttcatgtgat aatccttccg gcttccaggc
     9961 ggccaagttg gtggccggcg gcctggctgg tcatgatgcc cgatcccgtt gtcgaggacg
    10021 tacgaagaac ggtcgccggg aaatcgaaga taggttctca ttcattcctc ctacggcttg
    10081 gttaccgtaa cccgatagaa gacctggttc gtgggattgg ccgccacgct gaccacgaaa
    10141 ggcccggttt gtgcgcaggg gacgggagaa ctggccgcca cccacgcttg cgtggtgatc
    10201 agcctggacc gggtccatac ctggtacacg cgcccggcca cgccatccac ctcgaattcc
    10261 agcttgtttc ccgccgtttt ccactgcccg ccccccggcc gcatgtccca cgcccgaaag
    10321 tacgagtagg gattggtggg atccgtgccc gccacggact cgtctcgatt gacgaaggag
    10381 tccttgtcca ggtcgacatt gggatcgccg ctgtaccggt cgccgaagta ccgggccgcc
    10441 caggcgtcgt ccacgccatc gttgttgccg tccggatcgt tgccgtcaat caggccgtcc
    10501 ttgtccaggt ccggcgcggc ggccaacagg gcctgcacgg tggcggtgtc cgcctttttg
    10561 acccagaccc gaacctcgtc aatccggccc ttcaacgcat gggatccgtc ggggggatac
    10621 gtgtcgccca ggatcatcgg aagactgccg ccccgcgggc gggccagcac ggcggaactc
    10681 tccaatcgac cgtccatcca caaggtggcc gtgccgttgg tctcccattg cgccgccacg
    10741 tgatggaacc agccggtctt gatgttgctg gcggccacga cttgcttcgg gctcccggcg
    10801 ccggtggatt ccaggaacag cttgccggcc gaattcacgc cgagcaacca ttccgaggca
    10861 ttggtgtttc ccgcctggta cttgcccgcg atcccggcga acccggcggt cgcggtcggc
    10921 aaacggttgc agcggatcca ggccgacagg aaaaatgtct gggtcggttg cggagacagc
    10981 agggaggccg tgaccgcgac attcgccggg ccggccgcca gcgtcgcctg cgggcccagg
    11041 gcctccagtt cggcgccccg gtatctcggc gccggcgctt cgcgggttat cacgaggcct
    11101 tccgggggaa ctgcgcgcgc ggcgcgctcc aggtcggccc gtgtcacgcc tgggggcaaa
    11161 ggggcctccg gcgcctgccc gcgacgtaac ccggcgcagc ctccgacgca gacgatcagg
    11221 cccaccccgc caatccatgc catacccatc cggctcatgc ggccagtttt cattacatta
    11281 tcctcggttt cgcgggcaca ttccccgccc tacggagcaa gctcttccaa ccacaattcg
    11341 cccggcgccc attccgccgg cacttcgccc gacatcatgg tgaggacgct ctgcttttca
    11401 cccagttccg tgatggccgc catccctgcg acgctctcct gctcggccgt cgtgcggcga
    11461 agcagctgaa attggacgcc ggcccgggcc ccgtgcaggc gtccgacggc ggcgcgaagg
    11521 cgccccttct cgaccgattc cacgcacgcc gtcagcggga cctgctctat caaccgggtt
    11581 atcatggcgc ccgcgatccg acccgcggca gccatgacat cttcctgcgg gccgaccgct
    11641 tgcgaaaatg accccagggc cagcgtcgtc tccgtatcca ccagccgcat caacagctgc
    11701 tgcgagtctc cacgagagag gagcgtgccg agcagcaaat agctggcggg caggaccttt
    11761 ccgatcgcgg tgcgcgcatc cggctccgcc agctccgagg ctcccaggtc catctcctgc
    11821 aggacggttt ccagcgcatc ccgctccacg acccgaaccc gcccatcgtc ttccagggcc
    11881 gcgcgcagtg agatgcgata taacagggcg tcggcctcgt cgtcgctccc cccgcgcagg
    11941 ctgtcgggcc ccatgcaagc cacgatgcgg gggcgggccg cgcccaaatc cgccggctgg
    12001 gcgttgtgct tccgctcctc cagttgccgg atcaacccgc gcagttcttc gcggctattc
    12061 tgttcccttt cccggtccag gtagtcggcg acagcctcca gcaaggccgc attgcttgaa
    12121 ccggcggggg tcatcgccag caggcgatgg gccgcacccg tggcgccctg ttgactcaag
    12181 agcgcggcat cgcgcaacag ttcccgcgag gacggcgccg cggccgcttc gggcccgcgg
    12241 acggactgcc cggcggccgg gacggccacc gcctcttccg gaatggaagc gccctggcgg
    12301 acgaagacaa actcgccgcc ggcatggga
//
LOCUS       DAOOTK010000101        12182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3479_length_12182_cov_5.553311, whole genome shotgun sequence.
ACCESSION   DAOOTK010000101 DAOOTK010000000
VERSION     DAOOTK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3479_length_12182_cov_5.553311"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK634_10785"
     CDS             <1..424
                     /locus_tag="PK634_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HOW98262.1"
                     /translation="GLGAFMRLGRGEEQSGGRARASTLGDALEAVLGAAYLDGGLKAV
                     ERIYKHLFTALASGKESATAQDNPKGALQEYCQRRWKVGPAYRLLEVTGPGHARRFNV
                     EVRIGDRVAAEAHGPSKREAESRAALKALQELNESPSA"
     gene            complement(484..1131)
                     /locus_tag="PK634_10790"
     CDS             complement(484..1131)
                     /locus_tag="PK634_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOW98263.1"
                     /translation="MDEGKNSGAAVSAAVVLSVAAYIAAQMLADIASLKIGVVAGLAV
                     DMGTFIYPVTFTLRDVVHKLIGRRLARLLIVAAGVINLLMAAYLLWVARVPGDPVWGL
                     DAQFRAILAPVWRIVIASILAEVVSELLDTEIYHLFVTRVTRRFQWLRVLASNSISVP
                     VDSLLFSVGAFGWALPWGAVWQIFVFNMIVKYAVTLVSLPMIYLAPDRQSREDLV"
     gene            complement(1201..1860)
                     /locus_tag="PK634_10795"
     CDS             complement(1201..1860)
                     /locus_tag="PK634_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOW98264.1"
                     /translation="MLFPSLAGHPVARINLDDEAARWLARRAAAPGAPNPLLDPSVCG
                     RMLDEVHRRERVAWSYGGYLEDRRRLWRGSYLEKAGAFIHLGVDFNVPRGTEFVAGFP
                     AGIVVVDDDQDRDGGWGKRIILRPEIPGCDALLIYAHLQNVRRRPGEHAPAGAVLAEV
                     GGPPDNGNWHPHLHIQAIRRGLFEEILLDRFNELDGYGRAEELDALRASFPDPLPIVR
                     P"
     gene            complement(1868..3367)
                     /locus_tag="PK634_10800"
     CDS             complement(1868..3367)
                     /locus_tag="PK634_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW98265.1"
                     /translation="MTPKDTLKLTLPNDLSYLPNAQAFVVEAARRFGFSEAELMPIEV
                     GVEEAVTNVMKHAYDAEESRDFEIVCEKLPAGIRIIVREKGMPFDPAQVEAYRTGASI
                     EETSARGLGTFLMQHLMDEVTFHNLGTEGKETRLVKYRKDKTALESEQQERQAAATEP
                     AVIVEKIDYDVRGLDPREAIQVSRCAFKSHGYSFFDEHIYYPERLVAMNRSGEMISAV
                     AVTKERVFMGHAALVYQYPDDRIAELTFVFVNVEYRGQGAFNRLIEYLFTCPKTRGLR
                     GIYAYAVSNHVFTQKAMARYQVNDCGILLATSPSSWKFRGIPEAGAQRISVILGFKYM
                     GPPEKRRLYPPARHRAMIEKLYRNIGAADHEYAEPDSATLPPAGDADLLTGVNAAEGC
                     AEIFIRRYGADAVTAVRKALRQFCVQQVACVNLFLNLEDPATALLAAEFEKLGFFFAG
                     ILPCSRIGDALVLQYLNNVALDYGKIVAYSDVARELLAYIRELDPNADL"
     gene            3484..7830
                     /locus_tag="PK634_10805"
     CDS             3484..7830
                     /locus_tag="PK634_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOW98266.1"
                     /translation="MKARGHTGRGRFPRGAMAILLLIAAGPRVLSAAPLTFTSTNSGN
                     WHGTGSDYIWERSGTDHLLPTAGDTVNVCGYYENVFVTCNVSVATLNVRTGAYEGVLE
                     IYPSATLTVDNSGTWSGGRIGGGGGTFVNRGALLMPVTDRTVVVGTMINSNLITRSGP
                     GAFGTHAGALFYNATGAVFNLQSDAKMVHSTGGGGIPRFVNLPGSTFRKSAGAGTAQT
                     EIPFDLYGALLDIQSGTFHIGSSGNSSGAVYQVSVGAVLDVTPFYEDHFWSGAHTGTG
                     LGTIALHSNTISLSGTPVTFNFSSNLFHWWAGTFSCTHLDNKGFLTLDGDGVKSIKGY
                     MVNSNRIEHAGAGNFDVYWLNGGFQNAAGAVYNFQGDGGVANDGTIDNYGTFRKSGGA
                     GTSVVSGTFNNYGTIDVRSGTLNLRAVGNRPGTNYLVSPGATLDLSPQGDTTYSGTHS
                     ASGGGTVVLNGNGMQIAAANATFQFPTNTFLWRSGSIMNNILTNRGVIHLVGSGEKKV
                     HHFVNQGWVLQEGTGYFGIHQNGYMINAAGAVFEIRTDAGLARQAQIPTFYNEASGTV
                     RKVAGTGTCDFAAVFFNQGLLEAQAGMLLFSVTNTSFQSFIGQTGSVTRLAGGHLSSG
                     RCDFDGGLLTGTGMLRGSVYNNSGIIAPGNPAGTFMDLRCFEQGPAGQLHMDLAGAAP
                     GTGYDQLSITGYVYFGGTLRVSTTNGYDPAGHTYTLMTYGSLFGGRFDATNLPSLAAH
                     PGLHWMVHYGSNALVIAPAAYYTLSSGAFTGSEASGWATVVVTRSSAEAGDLALAYST
                     FDGTARSGLDYTAVTGTVTLTGAQRTNAFAIPLVNNAFDALSERSFGVQVGPQGAQAY
                     ELSGLATAMVTIVDDDAPVAAAFPFTETFESGAFSNYWSLYLGGPAGSLVLTTNEEPH
                     GARHVLMDNALTNAFALEELILTVNLAGQSEVWLSFYHKTINDEYHDMPDSFAQHTNA
                     DGVAVSADGVTWYTAQGLGGWNSLSLTHKQFIVSLDALRDRFGFGYNAAFKVKFQHYD
                     NRPAPDAGFAFDDIRLFRPVGYVRFATTNFQLRESAGIFTVQVARAGAATAAVSCAYY
                     ADNRDLWIGRDFSGPTGQLSFAAGQTGASFTVTLIDDQVPEAIERGRFLLTDFLGCAP
                     TSPSAARLVVEDNEATNVLDEGFDASVTPAGWTVRTNGGPGACWRFDRPGSWSNYTGG
                     LDNYAMANSDFAGATDMDTELRTPAFDFSAFGSVHLTYRCYFYHWSDEVGDIDYSTNG
                     AAGPWTPFWRYQNEDYEWVAQSINASALVAGRSNVMFRFHYYNAMNDWWWQLDEVDVY
                     GETDIDHDGLPDWWETGVGGNPTNVDPSADGDHDAFLNSHEYRAGTDPGDSNSYMRVQ
                     SGSRSGPGIVIRWPSVEGRWYRLERSSNLVSDAGFGANIATDLEASPPENTATDTTAG
                     ASGPWIYRIEVQ"
     gene            complement(7843..7940)
                     /locus_tag="PK634_10810"
     tRNA            complement(7843..7940)
                     /locus_tag="PK634_10810"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7883..7885),aa:Phe,seq:gaa)
     gene            complement(8276..9130)
                     /locus_tag="PK634_10815"
     CDS             complement(8276..9130)
                     /locus_tag="PK634_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98267.1"
                     /translation="MGWILIASAVLAACGLMFLLMDWAERVLCADGRIALFTRVQLIE
                     NALVVGILGAIGWRRRLWRMETLARVPRSAWLALMCGVIPAVLYIASLRMPAWVPWFG
                     EHGWLEGTTPILAIAAGSLFLCCAVRCRRKPPRDPAAVLAFAGLAAGAFFLAMEEIDW
                     GQVVFCWPTPEAVFSQNFQNVTNLHNFFNAVFPALYEVTGWAVAGALILSVARVRRSI
                     PWTGWRVLLPPLELAGLAGLVFLFRFWHEVFEQQFAWLLFFHAGMATGRAVSISTFPM
                     TQGREGPR"
     gene            complement(9151..9408)
                     /locus_tag="PK634_10820"
     CDS             complement(9151..9408)
                     /locus_tag="PK634_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98268.1"
                     /translation="MKKALGVFVMLGGLAISAVGLSNPVITAFSGSGTLEWSCPSNPL
                     EALACGLPVVTQDDAVRREVLGAARLQEVFEEVMNRAVPVP"
     gene            complement(9454..9627)
                     /locus_tag="PK634_10825"
     CDS             complement(9454..9627)
                     /locus_tag="PK634_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOW98269.1"
                     /translation="MGRLWAFAGGPCSCIVFAPFANWTANGYRLPTEAEWEKAARAGP
                     NSGLAKQTRPMGA"
     gene            complement(10193..10678)
                     /locus_tag="PK634_10830"
     CDS             complement(10193..10678)
                     /locus_tag="PK634_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98270.1"
                     /translation="MNTSECSMPHNGEWQPVRPEKAERVCLNELIQVLIGTFSEWAAW
                     KRVSLWAALEKEELAVKGMRSQLARAMQSLLAHAVDACREAHGEVSVMLRRDHDLACV
                     RVQHSREETSEALLRNDVRLDVAQRIVQEHGGCMEVAGYAGAGLAVTLRFLIVPADHA
                     T"
     gene            complement(10675..11895)
                     /locus_tag="PK634_10835"
     CDS             complement(10675..11895)
                     /locus_tag="PK634_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98271.1"
                     /translation="MDYPFISVHKPAAPVFGSRTALVRVPLVKITQNMKLAAILCWLA
                     MLVIIPGGGGVLVWSARHYAQRPAGAGEAKEIRAEEGADAVDEQREAVRTLIVQVMDR
                     GRTAYRPPVDLSKLPAARPLEFDFESVRQGDELRRSGDLCLEQGRVEEAVKACCAALK
                     KNPRNNNVWQSLGNGYLRAGNYEMAKRMLETAFRLGSVKADLMADLGALYRREGRLGE
                     ALALWDQCRQVAGSAPQADLNEGLAWLEAEDFERAALILERYVGRVPQDTAASRALAY
                     AQLQQGDWAEARRTLKKALAEAPGSAELRADAAAVAAHGWLAHETLEHLEQLARMTSP
                     DLAYQYLHYPAFAGFRKTMLGRGLEAVLLADTGRTVLSPEEVRERLAVDISPRFSHVP
                     GTSAASEGEPISSP"
     gene            complement(11899..>12182)
                     /locus_tag="PK634_10840"
     CDS             complement(11899..>12182)
                     /locus_tag="PK634_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98272.1"
                     /translation="ACAYSSLGRTEEAVAAVYAAYKAHPKALSLWMTDGQLDGIRGDP
                     RIVIVQNAARGAWRSSPPAPPAGLSLSSLPASWAAKRAETPGRLALQGW"
BASE COUNT         2143 a         4313 c         3795 g         1931 t
ORIGIN      
        1 cgggctcggc gcgttcatgc ggctgggccg cggcgaggag caatccggcg gacgcgcccg
       61 cgcctcgacg ctgggcgacg cgctggaggc ggtcctcggg gcggcctacc tggacggcgg
      121 actgaaagcc gtggaacgaa tctacaagca cctgttcacc gccctggcct ccggcaagga
      181 aagcgcgacg gcgcaggata atcccaaggg cgcgctgcag gaatactgcc agcggcgctg
      241 gaaggtcggc ccggcctacc gcctgctcga ggtcaccggc ccgggccacg cccggcggtt
      301 caacgtcgag gtccgcatcg gcgaccgcgt cgccgccgag gcccacggcc cgagcaagcg
      361 cgaggcggag tcccgcgccg cgctcaaggc gctgcaggaa ctgaacgagt caccgtcggc
      421 ctgacgatga tgcgaccgct gcagcgcgcc accgcgcggc gcggccgccg caggaccgac
      481 cggtcacacc agatcctccc gcgactggcg gtcgggcgcc aggtagatca tcggcaggct
      541 gaccagcgtg acggcgtatt tcacgatcat gttgaagacg aagatctgcc agaccgcgcc
      601 ccagggcagc gcccagccga acgccccgac ggaaaacagg aggctgtcca ccgggacgct
      661 gatgctgttg ctggccagca cccgcagcca ctggaagcgc cgcgtcacgc gcgtgacaaa
      721 caggtggtag atctcggtgt cgaggagttc gctgaccacc tcggccagga tcgacgcgat
      781 cacgatgcgc cagaccggcg cgaggatcgc ccggaactgg gcgtccaggc cccagaccgg
      841 gtcgcccggc acgcgcgcca cccagagcag gtaggcggcc atcagcaggt tgatgacccc
      901 ggcggcgacg atcagcagcc gggccagccg ccggccgatg agcttgtgga ccacgtcgcg
      961 cagggtgaag gtgacggggt agatgaatgt ccccatgtcc acggccagcc cggccaccac
     1021 gccgatcttg aggctggcga tgtccgcgag catctgcgcg gcgatgtacg ccgcgacgga
     1081 caggaccacg gcggccgaca ccgccgcccc gctgtttttt ccctcgtcca tggttccgcg
     1141 ccggcagtag gcataccaca aggccgggcc gcgtgaaaag gccggacccg ccgcgcccgg
     1201 ctacggccgc acgatgggca gcgggtccgg gaaggaggcc cgcagggcgt ccagttcctc
     1261 cgcgcgcccg tagccgtcga gctcgttgaa ccgatcgagc aggatctcct cgaacagacc
     1321 gcgccggatc gcctggatgt ggaggtgcgg atgccagttg ccgttgtcgg gcggcccgcc
     1381 gacctcggcc agcaccgcgc cggcgggtgc gtgttccccc ggccggcgcc ggacattctg
     1441 gaggtgcgcg taaatcagca gcgcgtcgca gcccgggatc tccgggcgca ggatgatacg
     1501 cttcccccag ccgccgtccc ggtcctggtc gtcgtccacg accacgatac ccgccgggaa
     1561 tccggcgacg aactccgtgc ctcggggcac gttgaaatcc acgccgaggt ggatgaatgc
     1621 gccggccttc tccaggtagc tgccccgcca gaggcggcgg cgatcctcca ggtaaccgcc
     1681 gtaggaccag gccacgcgct cccggcggtg gacctcgtcg agcatccgcc cgcagacgga
     1741 gggatccagc agcgggttcg gcgcgcccgg ggcggcggcc cggcgggcca gccaccgcgc
     1801 ggcctcgtcg tcgaggttga tccgcgccac ggggtgtccg gccagcgagg gaaagagcat
     1861 gggcctccta caggtcggcg ttgggatcga gttcccggat gtaggccagg agttcccggg
     1921 ccacgtcgga atacgcgacg atcttgccgt agtcgagggc gacgttgttg aggtactgca
     1981 ggaccagggc gtcgccgatg cgcgagcacg ggagaatgcc ggcgaagaag aagcctagct
     2041 tctcgaactc ggcggcgagc aacgcggtcg cagggtcctc caggttcagg aacaggttga
     2101 cgcaggccac ctgctgcacg cagaactgcc gcagcgcctt gcgaacggcg gtcaccgcgt
     2161 ccgcaccgta ccgccggatg aagatctccg cgcagccctc ggccgcgttc acgcccgtca
     2221 ggagatccgc gtccccggca ggcggaaggg tggcggagtc cggctccgcg tactcgtggt
     2281 ccgccgcgcc gatgttccgg tacagcttct cgatcatggc ccggtgccgg gccggcggat
     2341 agaggcgccg cttctccggc gggcccatat acttgaagcc gaggatgacg ctgatccgct
     2401 gcgccccggc ctccgggatg ccgcggaact tccacgacga ggggctggtc gccagcagga
     2461 tgccgcaatc gttgacctgg tagcgcgcca tcgccttctg ggtgaagacg tggttcgaga
     2521 cggcgtaggc gtagatgccg cgcaatcccc gcgtcttggg acaggtgaac aggtactcga
     2581 tcagccggtt gaaggcgccc tgcccgcggt actccacgtt gacgaacacg aaggtcagct
     2641 cggcgatccg gtcgtcggga tactggtaga ccagcgccgc gtgccccatg aagacccgct
     2701 ccttcgtcac ggcgacggcg gagatcatct cgccggaccg gttcatggcg acgaggcgct
     2761 cggggtagta gatgtgctcg tcgaagaagc tgtacccgtg cgacttgaag gcgcagcggg
     2821 agacctggat cgcctcgcgc gggtcgaggc cgcgcacgtc gtaatcgatt ttctcgacga
     2881 tgaccgcggg ctccgtcgcc gccgcctgcc gctcctgctg ctcggactcg agggcggtct
     2941 tgtccttgcg gtacttgacg aggcgcgtct ccttgccttc cgtcccgagg ttgtggaacg
     3001 tgacctcgtc catcaggtgc tgcatcagga aggtcccgag cccccgggcc gaggtctcct
     3061 cgatggacgc gccggtccgg tacgcctcca cctgggccgg gtcaaacggc atgcccttct
     3121 cccggacgat gatccggatg cccgcgggca gcttctcgca aacgatctcg aagtcgcggc
     3181 tctcctcggc gtcgtaggcg tgcttcatca cgttggtcac ggcctcctcg acgccgacct
     3241 cgatcggcat gagttccgcc tcggaaaacc cgaaccgccg ggccgcctcg accacgaagg
     3301 cctgggcatt cggcaggtag gagagatcgt tcggcagcgt cagcttgagc gtgtccttcg
     3361 gggtcatggt cccatcctcc agaggttcgc tgcatgtata ccatttccag ccggccaccg
     3421 gaagaaggac cggacgcgcc gccaccttga accgccccgg gccgttttgg tagggtgagg
     3481 accgtgaaag cgcggggtca caccgggcgc gggcgtttcc cgcgcggcgc gatggcgatc
     3541 ctgctgctga tcgccgccgg cccgcgggtc ttgtccgccg cccccctcac cttcacgtcc
     3601 accaactccg gcaactggca cgggacgggc agcgactaca tctgggaacg ctccggcacc
     3661 gaccacctgc tcccgacggc gggcgacacg gtcaacgtct gcggctacta cgagaacgtg
     3721 ttcgtcacct gcaacgtctc cgtcgccacg ctcaacgtcc ggaccggcgc ctacgaaggc
     3781 gtgctggaaa tctatccgag cgccacgctg acggtggaca actcgggcac gtggtccggc
     3841 gggcggatcg gcgggggcgg cgggacgttc gtcaaccggg gcgccctgct catgcccgtg
     3901 acggaccgca cggtggtcgt cggcacgatg atcaacagca acctgatcac gcgctccggc
     3961 cccggggcct tcggcacgca tgcgggcgcg ctgttctaca acgcgacggg cgccgtgttc
     4021 aacctgcaga gcgacgcgaa aatggtccac tcgaccggcg ggggcgggat accgcggttc
     4081 gtcaacctgc ccggctccac gttccgcaag tcggcgggag cggggacggc ccagacggaa
     4141 atcccgttcg atctctacgg cgcgctcctg gatatccagt ccggcacgtt tcatatcggg
     4201 tccagcggca acagctcggg cgcggtctac caggtctccg tcggcgccgt cctggacgtc
     4261 accccgttct acgaggacca cttctggtcc ggcgcgcaca cgggaacggg cctgggcaca
     4321 atcgcgctgc acagcaatac catcagtctt tccggcaccc cggtcacgtt caatttctcc
     4381 agcaacctct tccactggtg ggccggcacg ttcagctgca cgcacttgga caacaaaggg
     4441 ttcctgacgc tggacggcga cggcgtgaaa tccatcaagg gctacatggt caacagcaac
     4501 cgcatcgagc acgcgggcgc cgggaacttc gacgtatact ggctcaacgg cgggttccag
     4561 aacgcggccg gcgcggtgta caacttccag ggcgacggcg gggtggccaa cgacgggacg
     4621 atcgacaact acggcacgtt ccggaagtcc ggcggcgccg gcacctccgt cgtgtccggc
     4681 acgttcaaca actacggcac gatcgatgtc cgctcgggca cgctgaacct gcgggccgtc
     4741 gggaaccgcc ccggaacgaa ctacctggtc tcgcccggag cgacgctgga cctgtccccg
     4801 cagggcgaca cgacctactc cggcacgcac tcggcgagcg ggggcggcac ggttgtcctc
     4861 aacggcaacg gcatgcagat cgccgccgcg aacgccacgt tccagttccc gacgaacacg
     4921 ttcctgtggc ggtccggctc gatcatgaac aacatcctga ccaaccgggg cgtgatccac
     4981 ctggtcggct ccggcgagaa aaaagtccac cacttcgtga accagggctg ggttctgcag
     5041 gagggcaccg gctatttcgg catccaccag aacgggtaca tgatcaacgc cgcgggcgcc
     5101 gtgttcgaaa tcaggaccga cgccggcctc gcccgccagg cccagatccc cacgttctac
     5161 aacgaagcct ccggcacggt gagaaaggtc gccgggaccg ggacctgcga tttcgcggcc
     5221 gtcttcttca accaggggct cctcgaagcg caggccggaa tgctcttgtt ctccgtgacg
     5281 aatacgagct tccaaagctt tatcgggcag accggaagcg tgacgcggct ggcgggcggc
     5341 cacctgtcca gcgggaggtg cgacttcgac gggggcctgc tgacgggcac cgggatgctc
     5401 cggggctcgg tctacaacaa cagcggcatc atcgcgcccg gcaacccggc cggcaccttc
     5461 atggacctcc gctgctttga gcagggcccg gccggccagc tgcacatgga cctggccggc
     5521 gcggcgcccg gcacggggta tgaccagctc tccatcaccg gctacgtgta ttttgggggg
     5581 acgcttcgcg tgagcacgac caacgggtac gatccggcgg gccataccta cacgctgatg
     5641 acgtacggtt ccctgttcgg cggccggttc gacgccacca acctgccgtc gctggctgcc
     5701 catcccgggc tgcactggat ggtccactac ggctcgaacg cgctcgtgat cgccccggcg
     5761 gcgtattaca cgctttcgtc cggcgccttc acgggctccg aggcaagcgg ctgggcgacg
     5821 gtcgtggtga cgcgcagcag cgccgaggcg ggcgacctgg cgctcgcgta ctccacgttc
     5881 gacgggaccg cgcggtcggg cttggattac acggccgtca ccgggacggt cacgctgacc
     5941 ggcgcccagc gcaccaacgc gttcgccatc ccgctggtca acaacgcgtt cgatgccctg
     6001 tcggagcgct ccttcggggt gcaggtcgga ccgcagggcg cgcaggccta cgagctgtcc
     6061 gggctcgcga ccgccatggt gacgatcgtg gacgacgacg cgccggtcgc ggcggccttc
     6121 cccttcaccg agaccttcga atcgggcgcg ttcagcaact actggtcgct gtacctcggc
     6181 gggccggccg gcagcctggt cctgacgacc aacgaggagc cgcacggagc gcggcacgtc
     6241 ctgatggaca acgcgctcac gaatgcgttc gcgttggaag aactcatcct gaccgtcaac
     6301 ctggcgggcc aatccgaagt ctggctctcg ttctaccaca agacgatcaa cgacgagtac
     6361 cacgacatgc cggactcctt cgcccagcac accaacgccg acggcgtggc cgtcagcgcg
     6421 gacggcgtca cgtggtacac ggcccagggg ctcggcggct ggaactccct gtccttgacg
     6481 cacaagcagt tcatcgtctc cctggacgcg ctgcgcgacc ggttcggctt cggttacaat
     6541 gcggcgttca aggtcaagtt ccagcactac gacaaccggc ccgcgccgga cgccgggttc
     6601 gccttcgacg atatccggct gttccgcccg gtcggctacg tccgtttcgc gacgaccaac
     6661 ttccagctcc gcgagtccgc cggcatcttc accgtgcagg tggcccgggc cggcgcggcg
     6721 acggccgcgg tgagctgcgc gtattacgcc gacaaccggg acctctggat cggccgggac
     6781 ttttcgggcc ccaccggcca gctctccttc gccgcgggcc agacgggcgc gtcgttcacc
     6841 gtgaccctga tcgacgacca ggtgcccgag gcgatcgagc gcggccggtt cctgctcacg
     6901 gacttcctcg gttgcgcgcc caccagcccc tccgccgccc ggctcgtcgt cgaagacaac
     6961 gaggcgacca acgtgctgga cgagggcttc gacgcctccg tgacgccggc cggctggacg
     7021 gtgcgcacga acggcgggcc gggcgcctgc tggcggttcg atcgccccgg gagctggagc
     7081 aactacaccg gcggcttgga caactacgcc atggccaaca gcgatttcgc cggcgccacc
     7141 gacatggaca ccgagctgcg gaccccggcc ttcgacttct cggcgttcgg gagcgtccac
     7201 ctcacgtacc ggtgctactt ctaccactgg agcgacgagg tcggagacat cgactacagc
     7261 accaacggcg ccgccgggcc gtggacgccc ttctggcgct accagaatga ggactacgaa
     7321 tgggtcgcgc agtccatcaa cgcctccgcg ctggtcgcgg gccggtcgaa cgtcatgttc
     7381 cgcttccact actacaacgc gatgaacgac tggtggtggc agctcgacga ggtggatgtc
     7441 tacggggaga cggatatcga ccacgacggg ctgcccgact ggtgggagac aggcgtcggg
     7501 ggcaatccca ccaacgtgga cccgtcggcg gacggcgatc acgacgcctt cctcaacagc
     7561 cacgagtacc gggcgggcac ggacccgggg gacagcaact cctacatgag ggtgcagtcg
     7621 ggttcgcgct ccgggcccgg catcgtcatc cggtggccga gcgtcgaagg ccgctggtac
     7681 cgcctggagc ggtcctccaa cctggtatcc gacgccgggt tcggcgcgaa catcgcgacg
     7741 gacctggagg ccagcccgcc ggaaaacacc gcgaccgaca caacggcggg cgcctccggg
     7801 ccgtggatat accggatcga agtgcagtag gcggggaaaa gctggtggcc agagtcggga
     7861 tcgaaccgac gacatgtggg ttttcagcct aaacaggccg ccatcaatat gttacacatt
     7921 tcccgccgca gtatctgcac gagccgaaga atcaacagaa cataaaacct tcatttttct
     7981 tcggcatttc gcttgtttgc accttcaagc aaactcccgc ccctctttat aaggagtgga
     8041 gaaccgcttt ctttcggcaa ccttcttctt gactaaatca tgcacgaaaa tgcacgggaa
     8101 tgcaaagagc gttaaaatat tggcctctga cgtcctcccc aaaaactgga cagggcgacc
     8161 tttagcccgt aatccccgca actgcgggcg tgggatagag cccgcctgga tcaagtgcgc
     8221 ttgcgccgtc aaatcgtaga ggactactgc ccccgccctt tggggccttc cagcattagc
     8281 gcgggccttc cctgccttgc gtcattggga atgtagatat tgataccgcc ctccccgtgg
     8341 ccattccggc gtggaagaac aacagccatg caaactgctg ttcgaagacc tcgtgccaga
     8401 accggaaaag aaaaaccagg cccgccaaac cggccagttc cagcggcggc agcagcacac
     8461 gccaccctgt ccacgggatg ctccggcgga cccgcgccac cgacaggatc agcgccccgg
     8521 ccacggccca gcctgtgacc tcatacaggg cgggaaaaac cgcattgaag aaattgtgca
     8581 gattcgtcac gttctggaaa ttctgggaga ataccgcttc cggcgtcggc caacagaaca
     8641 cgacctggcc ccagtcgatt tcttccatcg ccaggaaaaa tgctccggcc gccaggccgg
     8701 caaatgcaag gaccgcggcc gggtcccgcg gcggtttccg cctgcagcgt acggcgcagc
     8761 aaagaaacag gctgcccgcc gctatggcca gaatcggcgt ggtgccttcc aaccagccat
     8821 gttccccaaa ccaaggcacc caggccggca tcctcaagga ggctatatac agcaccgccg
     8881 gaatcacacc gcacatcagc gccagccatg ccgagcgcgg cacccgggcc agggtttcca
     8941 tcctccacag ccgccggcgc cagcctatcg cccccaatat ccccaccacc agcgcattct
     9001 ctatcaactg gacccgggtg aacaacgcga tccggccgtc cgcgcacaac acccgttccg
     9061 cccaatccat cagcaggaac atcaagccgc atgccgccaa tacggcgctg gcgatcagga
     9121 tccagcccac tcccccggga cggctttcgg tcatgggacc ggcaccgccc ggttcatgac
     9181 ctcttcgaac acttcctgaa ggcgcgcggc gcccaggact tcccggcgga ccgcgtcgtc
     9241 ctgcgtcacc acgggcaacc cgcaagccag cgcctcgagc ggattggacg gacaactcca
     9301 ttccaaagtc ccgctgccag aaaacgcagt aatcaccgga ttggatagtc ccacggctga
     9361 aatggccaat ccgcccaaca tcacgaacac gcccaacgcc tttttcatgc cgtcatatcc
     9421 ttgccgcaag gccaaccttc aaagccaatc ggtttatgct cccattggcc tcgtttgttt
     9481 ggcaagcccg gaattcggcc cggcccgtgc cgccttttcc cactccgcct ccgttggtag
     9541 gcgatagcca ttggccgtcc aattcgcgaa cggcgcaaag acgatacagg aacaagggcc
     9601 gcccgcaaag gcccacaatc ggcccacagg ctcgatgccc gagccgatcc ggccggcgct
     9661 taatcggttt gtcggaatgt cgggtttgtc ggtgccccgg ggagaaaaag ccgcattcga
     9721 tcacccccgc acctggccgc cgccccgggc gactgtgggc tttattcgct tcgggggcca
     9781 tgcagcactt gacaacactt gcggacaata gaggacaggg aaacgcgggt atttgccagg
     9841 aaatatgctg aaaaaaacga ggtggccaga gtcgggatcg aaccgacgac acacggattt
     9901 tcagcctaac ctggccgcta tcaatatgtt gcgcgattcc tgtcacaaaa tctgcacgag
     9961 ccgaagcatc agcagaacag aaaatcatta tttttcttcg gcattcgatt gtttgcactt
    10021 tcaagcaagc tcccgcccct cttgataagg attagagcat attaagtaat agtgtagccg
    10081 cacgaggcaa accaatgggc ggcatcgtcg ggggtaatgg atagcaaggc gtgagcaatg
    10141 gccgcctcca gccttcgcag gtcaacttcg gaatccggga ttacccctcc cgtcaagtgg
    10201 cgtggtccgc cgggacgatc aggaagcgca gcgtgacggc gaggccggcc ccggcatagc
    10261 cggccacctc catgcacccg ccgtgttcct gcacgatccg ctgcgcgaca tccagcctga
    10321 cgtcgttacg cagcaacgcc tcgctcgttt cctcgcggct gtgctggacc cgcacacacg
    10381 ccaggtcatg atccctccgg agcatcacgc tcacctcgcc atgcgcctcc ctgcacgcat
    10441 cgaccgcatg ggccagcagg ctctgcatcg ccctcgccag ttggctccgc atgcccttca
    10501 cagcgagttc ttccttctcc agggcggccc agaggctcac ccgcttccat gcggcccact
    10561 cggagaacgt cccgatcaag acctggatca gctcattgag gcacactcgc tccgccttct
    10621 ccggccggac aggctgccat tcgccgttgt gcggcatgct gcattccgag gtgttcatgg
    10681 tgacgagatg ggctcccctt ccgaagcggc ggacgtcccg gggacgtgcg agaaccgcgg
    10741 cgagatatcc acggccaacc gctcgcgcac ctcctccggg gaaagcaccg tcctccccgt
    10801 atccgccaac agcaccgctt ccagcccccg gcccagcatc gttttccgga agcccgcgaa
    10861 ggccggatag tgcaggtatt gataggccag gtccggcgag gtcatccgcg ccagctgttc
    10921 caggtgctcc agggtttcgt gcgccagcca gccgtgcgcc gccacagccg ccgcgtccgc
    10981 gcgcagttcg gcggatcccg gcgcctccgc cagcgccttc ttcaaggtgc ggcgcgcctc
    11041 cgcccagtcg ccctgctgaa gctgcgcata ggccagcgcg cgcgacgccg ccgtgtcttg
    11101 cggaaccctc cccacgtatc gctccaggat cagcgccgcc cgctcgaagt cctccgcctc
    11161 cagccacgcc agcccctcat tcaggtccgc ctgcggcgcc gaccccgcga cctgccggca
    11221 ctgatcccac aacgccagcg cctcgcccag gcgcccctcc cttcggtaca gcgcccccag
    11281 gtctgccatg aggtccgcct tgacgctccc cagtcggaac gccgtctcca gcatccgctt
    11341 cgccatctcg tagttccccg cccgcagata gccgtttccc aggctctgcc agacattgtt
    11401 gttcctcgga ttcttcttca gcgccgcgca gcaggccttc acggcctcct cgacccggcc
    11461 ctgttccagg cagagatctc ccgaccgcct caactcatcg ccctgcctga cgctctcaaa
    11521 gtcgaattcc agcggccggg ccgccggcag cttggaaagg tccaccggcg gacggtaagc
    11581 cgtccggccc cgatccatga cctgcacgat caacgtgcgc accgcttccc gctgctcgtc
    11641 caccgcgtcc gcgccctctt ccgcccggat ctccttcgcc tcccccgccc cggccggccg
    11701 ctgcgcgtag tgccgcgcgc tccataccag cacgcctccg ccacccggga tgatgaccag
    11761 catcgccagc cagcacagga tggccgccag tttcatgttc tgcgtgatct tgaccagcgg
    11821 caccctcacc agcgcggtcc tgctcccgaa caccggcgcc gccggcttat gcaccgatat
    11881 gaacggatag tccatgggtt accatccctg aagcgccaac cgacccggtg tttccgcgcg
    11941 cttcgccgcc cacgatgccg gcagcgaact caaggagagc cccgccgggg gcgccggcgg
    12001 cgatgatcgc cacgcgccgc gggccgcgtt ctgcacgatc acgatccgcg gatctccccg
    12061 gatcccgtcc agttgcccgt ccgtcatcca caacgacagc gccttcggat gcgctttgta
    12121 ggccgcgtag accgccgcca ccgcctcctc tgtccgcccc aggctgctgt aggcgcaggc
    12181 cc
//
LOCUS       DAOOTK010000102        12029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3531_length_12029_cov_5.808418, whole genome shotgun sequence.
ACCESSION   DAOOTK010000102 DAOOTK010000000
VERSION     DAOOTK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3531_length_12029_cov_5.808418"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2280)
                     /locus_tag="PK634_10845"
     CDS             complement(<1..2280)
                     /locus_tag="PK634_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOW98273.1"
                     /translation="MTHAGKWKSWLWAGIGASVLALPAQAEVMVQWFNTTWREMIEKI
                     PLMAEIGYGSIYVPVPNKGGSQWSVGYDLHDRFDLGDEDQNGTIATKYGTKHELVRLV
                     EMAHRFGIRVYFDNVMNHNSFGVPGYDESTPITLYPDMLPEDFHLIQTGTHFWRKSGS
                     IQQVHYDTGDHGAIQHYTLSDLTDISNESGNWDIPGADLSFVRQPAEPDKYPDLTDPN
                     NYIQDADGNRVYKYCDTNRPSGDPASEDVNAYLIRAARWTMHETKCDGFRFDAAKHVI
                     PDFFGEWKGVGNWGSYLGYCGAIQAEYDILHGFNDGDTRNSLFDEDAPRDDALLFGEA
                     VPHKNLDLYDYVRRGMRLLDFDYEGTASYTLDGWGDQKYAGNLQNMGRLGPDYGVMQP
                     MNHDHPQFADMDMLSTWMILRDGIALVYTDGNNHSLPDQWGRVFPEKGQGNFLGQYQN
                     ANVIDRLPMLMDTPGPESEISRTVWIHQQFVRGWDDNKWSSDDVVVNQKVYSQDYKGT
                     SYNNNEGAVLLVAINDDKYSGQTHYVNPFTHFRPGTLLFNYATRHNGWDQDEWVRVGT
                     DWKVNLRVPPGHYAAYSIMVPGRTAVEPSSPILIEQPAGSLVGLIPVIRVDGLHGDED
                     WDDDGIPNDEDPVYIQRVAGSDPIRFVSRSDGLTVSAMIRLDEGVDVNGDGLDNPPGQ
                     ATDVFLGYEPMTFRFRRLGETYFGIWDALVSDMDSTDGNEADFFNRHNYESEYEVTNI
                     SATAFGEGYHMVRTRLFRKR"
     gene            2565..2921
                     /locus_tag="PK634_10850"
     CDS             2565..2921
                     /locus_tag="PK634_10850"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HOW98274.1"
                     /translation="MLYDGACPFCSREAAILKKADASSRVRFVDISDPVFNPAAYGLT
                     REEADAQLHFFDGEGRVSRAMDAVRAAYRAAGIGGRMAWTGAPLVRPLFDRLYRVFAR
                     NRLRWGARLSRRRPGG"
     gene            complement(2928..4355)
                     /gene="gatB"
                     /locus_tag="PK634_10855"
     CDS             complement(2928..4355)
                     /gene="gatB"
                     /locus_tag="PK634_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOW98275.1"
                     /translation="MKYLPTIGLEIHVQLKTRSKIFCGCPNEPGGEPNTHVCPVCLGY
                     PGTLPNVNGEAIRLTVLTGLMIGCRINPYSKFDRKNYFYPDMPKNYQISQYDKPLCLG
                     GSVEIEVEGRKKAVGITRIHLEEDVGKSMHFGSSSGVDFNRAGAPLMEIVTEPDIETP
                     EEAQAFLLALRQILLYGGVSDCNLEQGNLRCDVNCSVRPEGQSELGTKIEIKNMNTFK
                     GVFHALKHELPRQEEVLRSGGAIRQETRRWDDEKGVTEAMRSKEYAHDYRYFPEPDLM
                     PVVLPPETVEQWRATLPELPAKRRDRLVTEYGLPDYDAGVLVADKAVADFFEETARAS
                     GNPKAASNWVMTDVLRCLGEKQTELKDAKLTSVSLAGLIRLVDQKVINVPTARELFSE
                     LFEKGGDPTELVKTKGLAQVSDTGALEAYADEAIAENPGPVADYRAGKKPALQFLVGQ
                     IMKKSRGKANPRMVQELLQRKLGGA"
     gene            complement(4352..5815)
                     /gene="gatA"
                     /locus_tag="PK634_10860"
     CDS             complement(4352..5815)
                     /gene="gatA"
                     /locus_tag="PK634_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOW98276.1"
                     /translation="MAGLSNLTLHETADRLARGECTSEDLVRDLVAAIEQRDGKVGAY
                     VWVNVEDALARAREADAARLGGDRRPLLGIPLAVKDVLNVKDQPCACGSRILKGYVSP
                     YDATAIARLREQGAVFLGRTNMDEFAMGSTTENSALGVTRNPWDLARVPGGSSGGSAA
                     AVAAGEALAALGTDTGGSVRQPASFCGCVGLKPSYGRVSRFGLTAYASSLDQIGPMTR
                     DVRDAAMLLQAMAGRDPRDSSSVEIPVPDYSASLRNDLNGMKLGLPREYFIAGTDPEV
                     ERAVRAAVEQCRALGAEFVEISLPHTPYAIATYYVVATAEASANLARFDGVRYGARVE
                     GEDPIDMYGKTRAAGFGREVKRRIILGTYVLSSGYYDAYYLRAQKVRTLIRGDFEKAF
                     AGCDAILTPVVPTPAYRIGEKADDPLQMYLGDIFTVTANLAGICGLSVPCGFAGAGLP
                     VGLQILGPAFKEENILRVGYAYEQSTEWHLRKPNLES"
     gene            complement(5826..6131)
                     /gene="gatC"
                     /locus_tag="PK634_10865"
     CDS             complement(5826..6131)
                     /gene="gatC"
                     /locus_tag="PK634_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOW98277.1"
                     /translation="MTDTFRDDASRLDVRYVAHLARLHLSDEEAAVFQPQLEQVLGYV
                     RELGALDVEGVEPTAHAFPVQNVFRDDEVRPGLDHEAAMKNAPQERQGQFIVPKIIE"
     gene            6353..7300
                     /locus_tag="PK634_10870"
     CDS             6353..7300
                     /locus_tag="PK634_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HOW98278.1"
                     /translation="MTKATTHVLVTGGAGYLGSILVPGLLARGCSVTVVDNFLYGQTS
                     LLDCCFDERLTILRGDARDARLIQPLAAQADVIIPLACLVGAPLCARKPIEARSINLD
                     AILMLLKHTGAGQRILSPTTNSGYGIGQEGVFCTEETPLRPVSLYGKLKVELEEALLA
                     SGRAVSLRLATVFGISPRMRLDLLVNDFTYRAVTDRFVVLFEAHFKRNYIHVRDVARA
                     FLHAMDHFDAMKGQAYNVGLSEANLSKMELCLEIKKQAPDFTIMESPVGEDPDKRNYI
                     VSNEKIERTGYQPRVSLQQGIAELIKGYQVIRRSSYSNV"
     gene            7325..8275
                     /locus_tag="PK634_10875"
     CDS             7325..8275
                     /locus_tag="PK634_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOW98279.1"
                     /translation="MSFWSDKRVLVTGGTGFLGSHMMERLTLAGARHIRAVRHAEFDL
                     TREADVERLFAKHPADVVIHLAGLVGGILPNQQRPAEYLYRNLMMGTLVMHYAWKSGA
                     KKFVAAGAGCGYPLNAPMPLREDDLWTGIPQPESAPYSLAKRMLTIQGEAYHRQYGFN
                     AAVCIPGNIYGPHDNFNLHDAHVIPALVRKFVEAADRRADSVSVWGSGLATRDFVYAG
                     DVADGMLRAAERIEGFDVINLGAGRDTSVGEVVDLLTEITGFKGRVELDRSKPEGQPR
                     RLMDSSRALERLGWRAETGLRRGLELTVDWYRHHQDEARK"
     gene            8272..9291
                     /locus_tag="PK634_10880"
     CDS             8272..9291
                     /locus_tag="PK634_10880"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOW98280.1"
                     /translation="MNPYLRAKSPLRLSFAGGGSDIEAVYKEIGGAVLVATINKYSYV
                     SIRPHEDDRIRIRSLDFNVEVDYAARGEPPYDGVLDLAKACVEETGIRRGLDISLQTE
                     APPGSGLGSSSSMITALLAGLQVLTGRHMDPYELAEKAYDVERVKMKISGGKQDQYAC
                     SFGGFNLIEFHPDNVEVTPLRLRPDILNDVESHCMLCFTGRTRLSANLIDKQVAAFHE
                     AGSSSRAGTEKLKALAYEAKNLLLRGKISEFGHLLHEAHLAKKTMNPLVTDEFIDSLY
                     EEARRLGAFGGKILGAGGGGYLMLFAPIDNKQAIREKLEQLGGRFATFGFCPDGVQTW
                     TSACP"
     gene            9270..10511
                     /locus_tag="PK634_10885"
     CDS             9270..10511
                     /locus_tag="PK634_10885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98281.1"
                     /translation="MDQRMPVNEPAAPRPVIVSMFRYPIADHEAMNNIFPAMLKELAT
                     TSDVHHFSYRSEKPHALHGHPGIFFHEFPIGVRRGHNADKWLKTLLWFPLSLRVAWQA
                     RRLKAGFIYIEEYITFLPLLLRLVSGCPVVVSGADIFWDVYLGHGFFSGLARKFLLAV
                     DRFTWRRLRGIITRTEAMRRYMASQGVEAEKAFVVPEACETSFFHPMDRVAARREWDL
                     QEDDLVIVHHGILHPNKNLDHAFEYLRPAFSKHPGLRLWIAGDGPLRGYLERRASELG
                     MADRVRFLGWLPDVTRLNSLLNAADISLIMRHGGFSDHFQVTANLLHSLACGCAILAV
                     RLDGIAENAEEGVSALMFDPESGEEFSRQLERLIADPALRGRLREGAERVARERLDPR
                     RITDLWVRALRHFAGEEKTAT"
     gene            10508..11542
                     /locus_tag="PK634_10890"
     CDS             10508..11542
                     /locus_tag="PK634_10890"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOW98282.1"
                     /translation="MNTKWRERPVLITGGAGFLGANMARRLAAEGARVRAVDNLERGR
                     LEHLAEIRDRIEFIELDLRDPAAARRAAEGMQVVFHFAARVGGIQVYLDRPGSVLLNN
                     VLIDQNVFQAAVEARAASFVYAGSAHVYPEARQQAPDAAPLREEEAYPARPALTYGWG
                     KLLGEITLEALAREHPWFRVSLARIMGAYGYHQDTDPATGSAIPVFCRRAARWPEGAP
                     FRIRGTGRETRSYIFVDDVLDALLLSVEAQERLQVVGPFNLANEGRATIAEIAERVVQ
                     VSGKDIPLTFDPAVPTALWGQAADCSLARRLLGGWTPRVSLEEGLRRMYAHVGKREEP
                     RNRQNTRKRS"
     gene            11596..>12029
                     /locus_tag="PK634_10895"
     CDS             11596..>12029
                     /locus_tag="PK634_10895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW98283.1"
                     /translation="MQNDQARFFDQADAYLSEKVDRRILRIREFLDETWKRLASPGAV
                     LDIGCGNGAILNRLPAGVYKAGIDVSERLLDMAKAKGVETFLVNVDESPLPFPDGQFD
                     LVMATDVIEHVVHTDHLMNEINRVLRPGGVLFAGIPNVNQPIS"
BASE COUNT         1985 a         4016 c         3917 g         2111 t
ORIGIN      
        1 ccgcttgcgg aacagccgcg tgcggaccat gtggtagccc tcgccgaacg ccgtggcgga
       61 gatgttggtg acctcgtatt ccgactcgta gttgtgccgg ttgaagaagt ctgcctcgtt
      121 cccgtcggtg gaatccatgt cgctgaccag ggcgtcccag atgccgaaat acgtctcgcc
      181 cagccgccgg aagcggaacg tcatcggctc gtagcccagg aacacgtccg tcgcctggcc
      241 cggagggttg tccagtccgt cgccgttgac gtccaccccc tcgtccaacc ggatcatcgc
      301 ggacacggtc agcccgtccg agcgcgagac gaaacggatc gggtcgctgc cggcgacgcg
      361 ctggatgtag accgggtcct cgtcgttcgg gatcccgtcg tcgtcccagt cctcgtcgcc
      421 gtgcagcccg tccacccgga tcaccgggat caggcccacc agcgagcccg cgggctgctc
      481 gatcaggatc ggcgacgacg gctccacggc cgtccggccc ggcaccatga tgctgtacgc
      541 ggcatagtgc cccggcggca cgcgcaggtt taccttccag tccgtcccga cgcggaccca
      601 ctcgtcctga tcccagccgt tgtgccgcgt cgcgtagttg aagagcagcg tgcccggccg
      661 gaagtgcgtg aagggattca catagtgcgt ctgtccgctg tacttgtcgt cgttgatcgc
      721 gaccagcagg accgcgccct cgttgttgtt gtagctcgtg cccttgtagt cctgcgagta
      781 gaccttctgg ttcacgacca cgtcgtcgct ggaccacttg ttgtcgtccc agccgcgcac
      841 gaattgctgg tgaatccaga cggtgcggga gatttcgctc tcggggcccg gggtgtccat
      901 cagcatcggc agccggtcga tcacgttcgc gttctggtac tggccgagga aattgccctg
      961 gcccttctcg gggaacacgc ggccccactg gtccggcagc gagtggttgt tgccgtccgt
     1021 gtagaccagc gcgatgccgt cgcgcaggat catccaggtg ctcaacatgt ccatgtccgc
     1081 gaactgcgga tggtcgtggt tcatcggctg catcacgccg tagtcaggcc cgagccggcc
     1141 catgttctgg aggttgcccg cgtacttctg gtcgccccaa ccgtccagcg tgtacgacgc
     1201 ggtcccctcg taatcgaagt cgagcagccg catcccgcgg cggacgtagt cgtagaggtc
     1261 caggttcttg tgcggcacgg cctcgccgaa gagcagcgcg tcgtcccgcg gcgcgtcctc
     1321 gtcgaacagc gaattgcgcg tgtcgccgtc gttgaagccg tgcaggatgt cgtactccgc
     1381 ctggatcgcc ccgcagtagc cgaggtagct gccccagttc ccgacgccct tccactcgcc
     1441 gaagaaatcc gggatcacgt gcttggcggc gtcgaaccgg aacccgtcgc acttcgtctc
     1501 gtgcatcgtc cagcgcgcgg cgcggatcag gtaggcgttg acgtcctcgc tggccgggtc
     1561 gccggacggc cggttggtgt cgcagtactt gtagacccgg ttgccgtccg cgtcctggat
     1621 atagttgttc ggatcggtca ggtccgggta cttgtccggc tccgccggct ggcggacgaa
     1681 cgacaggtcc gcccccggga tgtcccagtt gccggactcg ttcgagatgt ccgtgagatc
     1741 gctcaacgtg taatgctgga tggccccgtg gtcgccggtg tcgtaatgaa cctgctggat
     1801 ggagccggac ttccgccaga agtgggtgcc cgtctggatc aggtggaaat cctccggcag
     1861 catgtccggg tagagcgtaa tcggcgtgct ctcgtcgtag cccggcacgc cgaacgaatt
     1921 atggttcatc acgttgtcga aatagacccg gatgccgaac cggtgggcca tctcgaccag
     1981 ccggaccagt tcatgcttcg tgccgtactt cgtcgcgatg gtgccgttct ggtcctcgtc
     2041 gccgaggtca aagcggtcgt ggagatcgta gccgacggac cactggctgc cgcccttgtt
     2101 gggcacgggg acatagatag agccgtagcc gatttccgcc atcagcggga ttttctcgat
     2161 catttcccgc caggtcgtgt tgaaccactg gaccatcacc tcggcctgcg ccggcaacgc
     2221 gagaaccgac gccccgattc cggcccacag ccacgatttc cattttcccg cgtgcgtcat
     2281 cggtccctca catcgttcag aaagaaagga tctccaaggc tgcagcatcc ccgcgacaga
     2341 cattgtgtga gttaaattac tcctatgccc gttttttgtt aaacgtttta ctatagaata
     2401 gtaaaccgat tcttcatcat tggcggctgt tgtataattc agtgttcaat aatcggttat
     2461 gaattgccat ttctgaaacc ggatcccttg cagttagatc catgatgaag caagttaatg
     2521 caatgaacga agacaggccg ctccagtcag aaatacgcgg cgtcatgctt tacgacggcg
     2581 cttgtccgtt ttgctcgcgc gaagcagcca tcttgaagaa ggctgacgcc tccagccgcg
     2641 tacggttcgt ggatatcagc gacccggttt tcaatccggc cgcctacgga ctgacccgcg
     2701 aggaggcgga cgcgcaactt cacttcttcg acggggaggg ccgcgtatct cgcgccatgg
     2761 acgccgtccg ggcggcctac cgcgccgccg gcatcggcgg aaggatggcg tggaccggcg
     2821 cgccgctggt ccgcccgctc ttcgaccgcc tctaccgcgt gttcgcccgg aatcggctgc
     2881 gctggggcgc gcgcctgtcg cggcgacgcc ccggcggttg atcgacgtca ggccccgccc
     2941 agcttgcgct ggaggagttc ctggaccatc cggggattgg ccttgccgcg gctcttcttc
     3001 atgatctggc cgaccaggaa ttgcagcgcg ggcttcttgc cggccctgta gtccgcgacc
     3061 gggccgggat tctcggcgat ggcctcgtcc gcataagctt ccagcgcgcc ggtatcgctg
     3121 acctgcgcca ggcccttcgt cttgaccagt tccgtcgggt ccccgccctt ctcgaacaac
     3181 tcgctgaaca gctcgcgcgc cgtgggaacg ttgatcacct tctggtccac cagccggatc
     3241 agcccggcca gcgaaaccga cgtcagcttc gcatccttca gttcggtctg cttttccccc
     3301 aggcatcgca ggacgtcggt catgacccag ttcgacgccg ccttgggatt gccggacgcg
     3361 cgcgcggtct cttcgaagaa atccgcgacg gccttgtccg ccaccagcac gccggcatcg
     3421 tagtccggca agccgtactc cgtcaccaac cgatcgcgcc gcttcgccgg cagctcgggc
     3481 agcgtcgcgc gccactgctc gacggtttcc ggcggcagga caacgggcat gaggtcgggc
     3541 tcggggaaat agcggtagtc gtgcgcgtat tccttggacc gcatggcctc ggtgacgccc
     3601 ttttcgtcgt cccaccggcg ggtctcctgc cggatcgcgc cgccggaccg cagcacctct
     3661 tcctgccggg gcagctcgtg cttcagcgcg tggaacacgc ccttgaacgt gttcatgttc
     3721 ttgatctcaa tcttcgtgcc cagctcggac tgcccctccg gccgcacgct gcagttgacg
     3781 tcgcaccgca ggttcccctg ctccaggttg cagtcgctca cgccgccgta gagcaggatc
     3841 tgccgcaggg cgagaagaaa ggcctgggcc tcctcggggg tttcgatgtc gggctccgtc
     3901 acaatctcca tcagcggcgc gccggcgcgg ttgaagtcca cgccgctgga cgagccgaag
     3961 tgcatgctct tgccgacgtc ctcttccaga tggatgcgcg tgatgccgac ggctttcttc
     4021 cggccctcga cctcgatctc caccgagccg cccaggcaca gcggcttgtc gtactgcgag
     4081 atctggtagt tcttcggcat gtccgggtag aagtagttct tccggtcgaa cttgctgtac
     4141 ggattgatcc ggcagccgat catcagcccg gtcagcacgg tcagacgaat ggcctcgccg
     4201 ttgacgttcg gcagcgtgcc gggatacccg aggcagaccg ggcagacgtg cgtgttcggc
     4261 tccccgcccg gctcgttcgg gcagccgcag aagatcttcg atcgcgtctt cagctggaca
     4321 tggatttcca ggcctatggt cggaagatac ttcatgactc gaggttcggc tttcggagat
     4381 gccactccgt ggattgctcg tacgcgtacc cgacgcggag gatgttctct tccttgaacg
     4441 ccgggcccag gatctggagg ccgacgggca ggccggcgcc cgcgaatccg cacgggacgg
     4501 acaacccgca gatgccggcc aggttcgcgg tgacggtaaa gatgtcgccc aggtacatct
     4561 ggagcgggtc gtccgccttc tccccgatcc ggtaggccgg cgtcgggacg acgggcgtca
     4621 ggatggcgtc gcaccccgcg aaggccttct cgaaatcgcc gcggatcagc gttcggactt
     4681 tctgcgcgcg caggtaatac gcgtcgtagt agccgctgct caacacgtac gtgccgagga
     4741 tgatccgccg cttcacctcg cgcccgaatc ccgcggcgcg ggtcttgccg tacatgtcga
     4801 tcgggtcctc gccctcgacc cgggcgccgt agcgcacgcc gtcgaaccgc gcgaggttgg
     4861 ccgacgcctc ggccgtggcg acgacatagt atgtcgcaat ggcgtacggc gtatgcggca
     4921 ggctgatctc gacgaactcc gcgcccagcg cccggcattg ctccacggcg gcccgcacgg
     4981 cgcgctcgac ctcgggatcc gtcccggcaa tgaagtactc gcggggcagg ccgagcttca
     5041 tgccgttgag atcgttccgg agcgacgcgg agtagtcagg cacggggatc tcgacgctgc
     5101 tggaatcccg cgggtcgcgc cccgccatcg cctggaggag catcgcggcg tcccggacgt
     5161 ccctggtcat cgggccgatc tggtccaatg acgaggcgta ggccgtcagc ccgaagcgcg
     5221 agacgcggcc gtaggaaggc ttgagcccga cgcagccgca gaacgacgcg ggctgccgga
     5281 cagagccgcc ggtgtccgtg cccagcgcgg ccagggcttc gcccgcggcg acggcggcgg
     5341 ccgagccgcc gctggaaccg ccgggcacgc gggccaggtc ccagggattg cgcgtgacgc
     5401 cgagcgcgga attctccgtg gtggacccca tggcgaactc gtccatgttg gtccggccca
     5461 ggaagacggc gccctgttcg cgcagccggg cgatcgctgt cgcgtcgtag ggcgagacgt
     5521 agcccttcag gatgcgcgag ccgcaggcgc agggctggtc cttgacgttg agcacgtcct
     5581 tgaccgccag cgggatgccc agcagcggac gccggtcgcc gccgagccgc gcggcgtcgg
     5641 cctcgcgggc gcgggccagc gcgtcctcga cgttcaccca tacgtacgcg ccgaccttgc
     5701 cgtcgcgctg ctcgatggcg gcgaccaggt cgcggaccag gtcctcggac gtgcattctc
     5761 cgcgggccag ccggtccgct gtttcgtgca gggtcagatt ggaaagcccg gccatgtcgg
     5821 cgatctcact cgatgatctt cggcacgatg aactgcccct gcctttcctg gggggcgttc
     5881 ttcatggcgg cttcatgatc caggcccggc cggacctcgt cgtcgcggaa cacgttctgg
     5941 accgggaagg catgcgcggt cggctccacc ccctcgacat caagggcgcc gagttcgcgg
     6001 acgtagccga gcacctgctc gagctgcggc tggaaaacgg ccgcttcctc gtcggaaaga
     6061 tgaaggcggg cgaggtgcgc gacgtagcgc acgtccagtc gcgaggcgtc atctcgaaaa
     6121 gtgtccgtca tgctccgatc ccatccattg gatgcaaacg ggcggaatat aagggtcacc
     6181 cacgccgacg gcaagccgac aaaccgacac acagcccatg atgcgctccg cccaatgtat
     6241 cacagtttat aaactgtgat acatagcttc tccggcattt cctgcggaca atgagcaaac
     6301 cctccccgcg cggaagttga cacccccgta ggccaacggt agggtaaatc gcatgactaa
     6361 agcaacgact catgtgctgg tcaccggggg cgcaggttat ctgggctcca tcctggtccc
     6421 cgggctgctg gcccgcggct gctccgtaac cgtcgtcgac aacttccttt atggacaaac
     6481 gtcccttctg gattgctgtt tcgatgagag actgaccatc ctccgcggcg acgcgcgcga
     6541 cgcgcggctg atccagccgc tggcggcgca ggcggacgtg atcatccccc tcgcctgcct
     6601 ggtcggcgcc ccgctctgcg cccggaaacc catcgaggcc cgctcgatca acctcgacgc
     6661 gatcctgatg ctcttgaagc acaccggcgc cggccagcgg atcctctctc cgaccaccaa
     6721 tagcggctac gggatcggcc aggagggcgt gttctgcacg gaggaaacgc ctctgcggcc
     6781 ggtctcgctc tacgggaaac tgaaggtcga actcgaggaa gccctgctgg cctccggccg
     6841 ggcggtatcc cttcggctgg ccacggtctt cggcatcagc ccgcggatgc ggctggacct
     6901 gctggtgaac gatttcacct atcgcgcggt gaccgaccgg ttcgtggtgc tgttcgaggc
     6961 gcacttcaag cgcaactaca tccacgtgcg cgacgtcgcc cgggcgttcc tgcacgccat
     7021 ggaccatttc gacgccatga aaggccaggc ctacaacgtg gggttgagcg aggcgaacct
     7081 gtccaagatg gaactgtgcc tggagatcaa gaaacaggcg ccggatttca cgatcatgga
     7141 gtcgccggtt ggggaagacc cggacaagcg caattacatc gtgagcaacg aaaagatcga
     7201 gcgcacggga taccagccgc gcgtgtccct gcagcagggc atcgcggaac tgatcaaggg
     7261 ttaccaggta atacggcgga gcagttactc gaacgtgtga gccggacgga gggtctacgg
     7321 tatcatgtct ttctggagcg acaagcgggt cctggtgacg ggcggcacgg gcttcctcgg
     7381 gtcgcacatg atggagcggc tgacgctggc cggcgcgcgc catatacggg ccgtccgcca
     7441 cgcggaattc gacctgacgc gcgaggcgga tgtcgagcgg ttgttcgcga agcatccggc
     7501 agacgtggtc atccacctcg ccgggctggt cggcggcatc ctgcccaacc agcaacgccc
     7561 ggcggagtac ctctaccgga acctgatgat gggcacgctg gtcatgcact acgcctggaa
     7621 gagcggcgcg aagaagttcg tcgccgccgg cgcggggtgc gggtatccgc tcaacgcccc
     7681 catgccgctc cgggaagacg acctctggac gggcatcccg cagcccgaaa gcgcgcccta
     7741 ctcgctggcg aagcgcatgc tgaccatcca gggcgaggcc tatcaccggc agtacggctt
     7801 caacgccgcc gtctgcatcc cgggcaacat ctacgggccc cacgacaact tcaacctcca
     7861 cgacgcgcac gtcatccccg cgctcgtgcg caagttcgtc gaggccgcgg accggcgcgc
     7921 ggactccgtg agcgtctggg gcagcgggct cgccacgcgc gattttgtct acgccggcga
     7981 tgtggccgac ggcatgctcc gcgcggcgga gcggatcgag ggcttcgacg tcatcaatct
     8041 cggcgccggc cgcgacacat ccgtcggcga ggtcgtggac ctgctgacgg agatcaccgg
     8101 gttcaagggg cgcgtggagt tggaccgcag caagcccgag ggccagccgc gccggctcat
     8161 ggactcgagc cgggcgctcg aacggctggg ctggcgggcg gagaccggcc tgcgccgcgg
     8221 cctggaactg accgtggact ggtaccggca ccaccaggac gaggcgcgga aatgaatccc
     8281 tacctgcggg cgaagtcccc gctgcgcttg agcttcgcgg gcggcgggag cgacatcgag
     8341 gcggtctaca aggagatcgg cggcgcggtg ctcgtggcga cgatcaacaa gtacagctac
     8401 gtctcgatcc gcccgcacga ggacgaccgg atccgcatcc gctcgctcga cttcaacgtc
     8461 gaggtcgact acgccgcccg gggcgaaccc ccgtacgacg gggtgctgga cctcgccaag
     8521 gcctgcgtgg aggaaacggg catccggcgc ggcttggata tctcgctgca gaccgaggcg
     8581 ccgccgggca gcgggctcgg cagttcgtcc tcgatgatca ccgcgctcct cgccgggctc
     8641 caggtcctga ccggccggca catggacccc tacgaactgg cggaaaaagc ctacgacgtc
     8701 gagcgcgtga agatgaagat cagcggcggg aagcaggacc agtacgcgtg cagcttcggc
     8761 gggttcaacc tcatcgagtt ccacccggac aacgtcgagg tcaccccgct gcgcctgcgt
     8821 cccgacatcc tcaacgacgt cgaaagccac tgcatgctgt gcttcaccgg ccgcacgcgg
     8881 ctctcggcca acctgatcga caagcaggtc gcggcgtttc acgaggccgg cagttccagc
     8941 cgcgccggga cggagaagct caaggcgctg gcctacgagg cgaagaacct cctgctgcgc
     9001 ggcaagatca gcgagttcgg gcacctcctc cacgaggccc atctcgccaa gaaaacgatg
     9061 aacccgcttg tcaccgacga gttcatcgac tcgctctacg aggaggcgcg ccggctcggc
     9121 gccttcggcg ggaaaatcct gggcgcgggc ggcggaggct atctcatgct cttcgcgccc
     9181 atcgacaaca agcaggcgat ccgcgagaag ctggagcagc tgggcggccg cttcgccacg
     9241 ttcggatttt gtcccgacgg cgtccaaaca tggaccagcg catgcccgtg aacgagccag
     9301 ccgcgccgcg ccccgtcatc gtctccatgt tccgctatcc catcgccgat cacgaggcga
     9361 tgaacaacat cttcccggcc atgctgaagg agctggccac gacctcggat gtgcaccatt
     9421 tctcctaccg gtcggagaag cctcacgccc tgcacggcca cccgggcatc ttcttccacg
     9481 aattcccgat cggcgtccgg cgcgggcata acgcggacaa gtggctcaag accctgctct
     9541 ggttccccct ctccctgcgc gtggcctggc aggcgcggcg cctgaaggcc ggcttcatct
     9601 atatcgagga atacatcacc ttcctgcccc tcctcctccg cctcgtgtcg ggatgcccgg
     9661 tcgtggtcag cggggccgac atattctggg atgtctatct cgggcacggg tttttctcgg
     9721 ggctcgcccg gaagttcctg ctggccgttg accgcttcac ctggcgtcgg ctgcggggaa
     9781 tcatcacccg caccgaggcc atgaggcgct acatggcctc gcaaggcgtc gaggcggaga
     9841 aggcattcgt ggtcccggaa gcgtgcgaaa cgagtttttt ccatcccatg gaccgcgtgg
     9901 cggcgcggcg ggagtgggac ctgcaggagg acgacctggt catcgtccac cacggcattc
     9961 tgcatcccaa caagaacctg gatcacgcct ttgagtacct gcgcccggcc ttttcaaaac
    10021 atcccgggct ccggctctgg atcgccggcg acgggccgct ccgcggctac ctcgaacggc
    10081 gggcctcgga actgggcatg gcggaccgcg tccgctttct cggctggctt ccggacgtga
    10141 ccaggctcaa ctcgttgctg aacgccgcgg acatttccct gatcatgcgc cacgggggct
    10201 tctccgacca tttccaggtg acggccaacc tgttgcacag cctggcctgc gggtgcgcca
    10261 tcctcgccgt ccgcctcgac ggcatcgccg agaacgccga ggaaggcgtg agcgccctga
    10321 tgttcgaccc cgagtccggc gaggagttca gccggcagtt ggagcgcctg atcgcggacc
    10381 cggcgctgcg cggacggctg cgcgagggcg cggagcgggt cgcccgggaa cggctggacc
    10441 cgcgccggat cacggacctg tgggtccgcg cgttgcggca tttcgcgggc gaggagaaga
    10501 cggcgacatg aatacgaaat ggcgggagag gccggtgttg atcaccggcg gcgcgggatt
    10561 cctgggggcc aacatggccc ggcggctggc cgcggaaggc gcccgggtgc gggccgtgga
    10621 caacctggag cggggccgcc tggagcacct cgcggagatc cgcgaccgca tcgaattcat
    10681 cgagctcgac ctgcgcgatc cggcggccgc gcgacgcgcc gcggagggca tgcaggttgt
    10741 gtttcatttc gccgcgcgcg tcggcggcat ccaggtctac ctggaccgcc cgggctcggt
    10801 cctgctgaac aacgtgctca tcgaccagaa cgtcttccag gccgcggtcg aggcgcgcgc
    10861 ggcgtcgttc gtgtatgccg gcagcgcgca cgtctacccg gaagcccgcc agcaggcccc
    10921 ggatgccgcc cccctccgcg aggaggaggc ctatccggcc cggccggcgc tgacctacgg
    10981 gtggggcaag ctgctcggcg agatcaccct ggaggcgctg gcccgggagc atccctggtt
    11041 ccgcgtctcg ctggcgcgga tcatgggcgc ctacggctac caccaggaca ccgatcccgc
    11101 gacgggctcg gcgatccccg tcttctgccg gcgggcggcg cggtggccgg aaggcgcgcc
    11161 gttccgcatc cgcgggacgg gacgcgagac gcgttcatac attttcgtgg acgacgtgct
    11221 cgacgcgctg ctgttgagcg tggaggcgca ggagcggctc caggtggtcg ggcccttcaa
    11281 cctggcgaac gagggacggg ccaccatagc ggagatcgcg gaacgggtcg tccaggtgtc
    11341 cggcaaggac atcccgctga ccttcgatcc cgccgtgccg actgcgttgt ggggccaggc
    11401 ggcggattgc tcgctcgcgc gcaggctgct gggcggctgg acaccccgcg tatcgctgga
    11461 ggaaggcctg cgcaggatgt atgctcacgt cgggaaaaga gaggaaccac ggaataggca
    11521 gaacacacga aagaggagct gaaagggatt cactttccgt atctttcgtg attggctctc
    11581 tgacaggaat catccatgca aaacgaccag gcgcggtttt tcgaccaggc ggacgcctat
    11641 ctctccgaga aggtggaccg gcgcatcctg cgcattcgcg agttccttga cgaaacgtgg
    11701 aagcgtctcg cctcgccagg cgccgtcctg gacatcgggt gcggcaacgg cgcgatcctg
    11761 aaccggctcc cggccggcgt ctacaaggcc ggcatcgacg tctccgagag gctgttggac
    11821 atggcgaagg ccaaaggcgt ggaaacgttc ctcgtgaacg tggacgaatc gcccctgccc
    11881 ttccccgacg ggcagttcga cctggtcatg gcgacggacg tcatcgagca cgtggtccac
    11941 accgaccacc tgatgaacga gatcaaccgc gtgctccggc cgggcggcgt cctgttcgcg
    12001 ggcatcccga acgtcaacca gcccatctc
//
LOCUS       DAOOTK010000103        11977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3551_length_11977_cov_5.829391, whole genome shotgun sequence.
ACCESSION   DAOOTK010000103 DAOOTK010000000
VERSION     DAOOTK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_11977_cov_5.829391"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /gene="fni"
                     /locus_tag="PK634_10900"
     CDS             complement(<1..1024)
                     /gene="fni"
                     /locus_tag="PK634_10900"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOW98284.1"
                     /translation="MDPINERKLAHLRIIESDPDSDRRRHYFDAIRLKHRALPELNLA
                     EVDPSIEFMGRRLSFPLLISSMTGGDHEILRRINRNLAIAAEKAGVAMGVGSQRVLFS
                     NPAARASFEIRPHAPTTLLLANLGAVQLNRGFGIEECREALAVSGADALCLHLNPLQE
                     AVQPRGDTDFAGLAEKIGGIVRALGRPVVVKEVGAGISREDAELLLRAGVRYVDVAGA
                     GGTSWSRVEHHSLAESEDDPLGLVFQDWGIPTPDALRALNPCRDRLTLIASGGLRNGI
                     DLVKALALGASLCGMAAPFLRPAMESAEAVGMLLARLKREFTTALFLLGIRRAADLVG
                     NESVLRG"
     gene            complement(1109..2149)
                     /locus_tag="PK634_10905"
     CDS             complement(1109..2149)
                     /locus_tag="PK634_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012730718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOW98285.1"
                     /translation="MSVDNPTDAASIPLRWVGPIRLAGPEATGEVEVPLATFETPLWP
                     SVARGARVSMATEGIRAVVVDERMTRSVLVEAPNAAEAVRAGADLRRRKPDVAAVVEK
                     TSRFLKFLDLHVQNAANLLYVRLEGHPGDASGHNMLTRAAEAMLNWILREYPALKYVS
                     ISGNYCTDKKPSAVNGILGRGRHVIAELVVPRAVCEKLLKTTPEKLADLNLKKNLIGT
                     MLAGGLRSANAHFANMLLAFYLATGQDAANIVEGSQGLTHAEARGGDLYFSVSIPHLV
                     VGTVGSGKDLAFVRAHLERMGCAGGGGARRLAILCAAAVLCGELSLLAAQTNPGELMR
                     AHLKLERKSHRG"
     gene            2292..6935
                     /locus_tag="PK634_10910"
     CDS             2292..6935
                     /locus_tag="PK634_10910"
                     /inference="COORDINATES: protein motif:HMM:NF023351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HOW98286.1"
                     /translation="MPWRSLLLAIVSLSLAAAPSDGASKKKKAADSQPAVEKKAARKK
                     TSRAKQEKKNEPAVETQQGAAALPPAELTAGVQFRDDEAEGLGDILVPAWRFPSGLLF
                     VNPRATRTDSDAEEYNVGLGYRHLFPGRAIILGANGYYDYRDTGHSTYDQWGAGVELL
                     SRWLDARANGYWPEDTRHTVAEASETSSSRSTRTEEGWTDPYAAGHEIAQSYVVEQTT
                     RTTTTTRYYEQYEQALDGWDAEVGLRLPLPVRPEVLEPRVFGGYYRYDAEYGEDIEGF
                     KARVELRLLASVFLDAAWYEDEDLTGSDYYVGGRFTVPLDLAAAAHGRNPFAPLRSRL
                     RGEARGFDARLTEMVMRDPQIRLETSEFIERADLARVDRRKHTETRQEDYTLMADVNF
                     VSGDAGSRGGDGTAENPFRAIQPGVDSAFGERNVYVYNSSRAYRENVELTPGVTLWGS
                     GCLVPGYNGQSFGSGIQPTVDGHGGGPAISMANRTTIRGFRITNTGRGGSRVEDLPVF
                     GPTDVSGVGVLGRDATDLRVECNTIAGADVGALFARQGDFSLAFLDNDVLDNRDAGLM
                     IGGQGSSGTFDVLVDGSRFERNGIGLMSVAWNYDEAFLTIRNSAFERNGAAGASILMD
                     QNGFSSVEIADSQAERNAGPGFDINITASDEALVALSGVGSHHNGGAGVQVLTLGTGQ
                     AVATIENVQAHGNAGPGIDLAFLDNGEVMASVNRAHADGNAGPGIRITATAADTVLAS
                     VANSWAAANGGGGVEILVDDTAMALALVGGSQAHDNAGGPGFNVLIENTDVALANLSG
                     LTANGNEGGGILLNMQSPFAAIGVIGLPDALAGLLGGVMETAGGFLPAETAMLLGHAG
                     PVEAIGNQGLGVTAIIDGDMIAGGAIFDIHAENNVGGGVRGLVTSSNGFAVGLGGSSE
                     NIMEIAQLGADIAEFFGLDIPVMPSPGGRTVVNNNLGPGIGLGVMGNMAAVGALVGVA
                     ADGNLGLGAGVATLSDGISVSAAARILALNTTGDGLVVEADGPTAAVGVVADVQALGN
                     AGNGIITDINSDEGYAAALFASTDPLRPVAELLGELLIGEPLTVPGQPFGPVIASGNG
                     EDGIAARVNGREAGIGLFLDTQATGNGGDGMDVSVQSSEGYAVSAFLSSDYLFNLASG
                     FFGPIDYEPLGGIIASDNDGHGIRLWQNGLAGAYAVLAGLQANDNEKDGLNARSNSEE
                     GDAMTAVIVADAMDNGGQGFFTENNAGLDSLAAFVGVWASGNERQGIRAVGTSVNANA
                     YAILAGVETVDNGAAGASVELGATGDAVACLTDVDSCLNEGRGAAVTLNAAGNAVLWA
                     GPNALNQFNSQYSPLFGVFNLAAFLIPQDAVTLDNNGGAGLYADLRSAAGNVVMELED
                     ARAIGNDKDGFNLQMAAANGSISATLRNAVAGENGARGFDVWAEGGGGTLDLYLEHAE
                     ATGNTNDGARIRANYSGPVNVYGEQVVAINNGGAGARMDILAGGTLNMDFGGGALGSP
                     GQGQFFGNGSRDFRNAGAGTVFAEQNWWGVDPPSAGQFFGAVDYTPWLTEPPP"
     gene            7262..8854
                     /locus_tag="PK634_10915"
     CDS             7262..8854
                     /locus_tag="PK634_10915"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOW98287.1"
                     /translation="MSAAKNSEKNRVIIFDTTLRDGEQCPGASMNIHEKLEVARQLAR
                     LNVDVIEAGFPIASPGDFEAVKAVAEQVKGPYIAGLARCINKDIERCAEAVAPAGKKA
                     YIHTFLATSAIHRQFKLKKAKHEIVKQAVASVKHARTFCDRVQFSPEDASRTEPEFLA
                     EVCEAVIDAGATTVNIPDTVGYAVPIEFGRLIAYLVEHVKNIHRAIIHVHCHNDLGLA
                     VANSLAAVRNGARGVECTINGLGERAGNCSLEEVVMGLKVRGDAFGRLWTSVKTRELY
                     RTSRLVSQMSGMVVQRNKAIVGANAFAHEAGIHQDGILKERTTYEIMRPEEVGISGSD
                     MVLGKHSGRHALFVHLGRMGFQVAEDQKESVYNQFKELCDKKKYIYDDDLIAIMRERI
                     SDIPQVYTLDYFHVATGTSTVPTATVRLKKGEEVFQDSACGDGPVDATLKTIDRITGT
                     PGTLEDYSLQAVTRGEDAVGEVSVTVAFGKDMVSAKAASTDIVEASAKAYLNALNSHL
                     FAQQTAKGPKKKAAGRRQGRQP"
     gene            8998..10320
                     /gene="ssnA"
                     /locus_tag="PK634_10920"
     CDS             8998..10320
                     /gene="ssnA"
                     /locus_tag="PK634_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOW98288.1"
                     /translation="MSTLLVKNGLLVTLNEHGEVIENGSVYVEDARIMEVGHFHENRY
                     KADQVLDAGGRLVMPGLINAHHHLYSTFACGFSPPGQPAENFKEILEHLWWKLDFALT
                     EEDVYYSALLPLVDCVKHGVTTIIDHHASPSCRDGSLDLIEKAVREVGINASLCYEVS
                     DRNVEGGGVEENERFIRKCAAAADDQVTALFGLHASMTLGAKTLERCAAIGRAHGAGF
                     HVHVAEDLIDQEVAQKEFGKRCMERFRDAGMTGPRSIFVHGVHLDDGELDILAETHSI
                     HVHNPESNMNNAVGTARLARLWKRGILTGLGTDGMASNMIASARAAYLLQRHLEHDPR
                     VAFGECCDMLLKNNRTICERLFKNKRGVLAAGALADLAIFDYTPFTPLSGANFYGHLL
                     FGLYQSRVSATVARGRVLMKDGVLPHLDEAALRHKAKDLAAKMWKRIK"
     gene            complement(10352..>11977)
                     /locus_tag="PK634_10925"
     CDS             complement(10352..>11977)
                     /locus_tag="PK634_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98289.1"
                     /translation="DQCGTEGHGGGFYLENTTADLTRFTGWLNVAGGNGGGLYATGSW
                     LQVSGMDFRHNAAPNPMGPPASGLGGAVALFSSMMTITNGPPGDAFATPRFAGNSVST
                     NGGGGGAVYVGAGSDFTAFNAVFETNRAVHGGALRAEGAPVSLLHNRLENNTADEGGG
                     IFVENTILLLVGGHLLGNAATNEGGALYGLHAVIRGYVVEAPYYTTAEGWSLSFQGNL
                     AHNGGAMHLDYCDTIISHAAFISNSAPTYASGLGIVGGQAHVAHTLITGGTQPAGWGG
                     LPGAAIAWAGPSTGTALFCTLAGNLGDGLRLYNDIQLSVSNSIVWGNQSWEISTNGLA
                     GGSLVVRHSDIGGGWPGAGNIDAYPEFYPNHHLRHGSPCINAGGALGSLYWLWDDTDA
                     DGESRLANTPDMGCDEFRDSDGDRMPDVVETHTGAYTSETDMGTDPNDPDTDGDDSPD
                     GEEWIADTDPTRGDDNLRILSITASTVAPGGVDVQWAGGTQADQVLEWAGDPGGPWQG
                     CAWYSAPTPKMNQGAFAIGSPTGLIFRVRAYRP"
BASE COUNT         2024 a         4039 c         4140 g         1774 t
ORIGIN      
        1 agccgcgcag gacggattcg ttcccgacca gatcggccgc tctccgtatc cccagcagga
       61 aaagcgcggt ggtgaactcc ctcttgagcc gggccagcag catgcccacg gcctcggcgg
      121 attccatggc cggccgcaag aacggcgccg ccatgccgca gagcgaggcc ccgagcgcca
      181 gggccttgac caggtcgatg ccgttccgca ggccgccgga tgcgatcagc gtcagacggt
      241 cgcggcacgg gttgagcgcc cgcaaggcgt cgggcgtcgg gatgccccag tcctggaaga
      301 ccaggcccag gggatcgtcc tcgctttccg cgaggctatg gtgctcgacg cggctccagg
      361 acgtgccgcc cgcccccgcg acgtccacgt agcggacccc tgcgcggagc agcaactcgg
      421 catcctcgcg ggaaatcccg gcgccgacct ccttgaccac caccggccgg cccagcgccc
      481 ggacgatgcc gcctatcttt tcggccagtc ccgcaaaatc ggtatcgccc ctcggctgga
      541 cggcctcctg gagcgggttg aggtgcaggc agagggcatc cgccccggac acggccagcg
      601 cctcgcggca ttcctcgatg ccgaagccgc ggttgagttg gacggcgccg agattggcga
      661 gcagcagcgt cgttggagca tgcgggcgga tctcgaaact cgcgcgggcc gccggattgg
      721 aaaacagcac ccgctgcgat ccgacgccca tcgccacgcc ggccttttcc gcggcgatcg
      781 ccaggttgcg gttgatccgc cgcaggattt cgtggtcgcc gccggtcatc gaggaaatca
      841 gcagggggaa agaaagccgc cggcccatga attcgattga aggatccacc tcggccaggt
      901 tcaactcggg cagcgcccgg tgcttcaggc ggatggcgtc gaagtaatgg cgccggcggt
      961 ccgagtccgg gtcggactca atgatccgca ggtgcgccag cttccgctcg ttgatcggat
     1021 ccatgttgac cgcagattac gcggatgggc gcggattaca agcccacgcc ggcctcctga
     1081 ccgccttctc tatccgtacc catccacgct atccgcggtg gctcttgcgt tccagcttca
     1141 ggtgcgcgcg catgagttcg ccggggttgg tctgggcggc gaggagggag agttcgccgc
     1201 acaggaccgc cgcggcgcac aggatcgcca ggcgccgcgc cccgccgccg cccgcgcagc
     1261 ccatccgctc caggtgcgcg cgcacgaaag ccaggtcctt gccgctgccg accgtcccca
     1321 cgacgagatg ggggatactg accgaaaagt agaggtctcc gccgcgcgcc tcggcatggg
     1381 tcaggccctg ggagccctcg acgatgttcg ccgcgtcctg cccggtggcc aggtagaacg
     1441 ccagcagcat gttggcgaag tgggcgttgg cgctccgcag cccgccggcc agcatcgtgc
     1501 cgatcaggtt cttcttgagg ttcaggtcgg cgagcttctc cggcgtcgtc ttcagcagct
     1561 tctcgcagac cgcgcgcggc acgaccagct ccgcgatcac gtgccggccg cgccccagga
     1621 tgccgttgac ggcggagggc ttcttgtccg tgcagtagtt gccggaaatg gagacgtatt
     1681 tcagcgcggg gtactcacgc aggatccagt tcagcatcgc ctcggcggcg cgggtgagca
     1741 tgttgtggcc ggaggcgtcg ccgggatggc cctcgagccg gacatagagg aggttggccg
     1801 cgttctgcac gtggaggtcg aggaacttca ggaaccggct ggtcttctcg acgaccgcgg
     1861 cgacgtccgg cttgcggcgc cgcaggtcgg cgccggcgcg gacggcctcg gcggcgttgg
     1921 gcgcctcgac gaggacggag cgcgtcatgc gctcgtccac gacgaccgcg cggatcccct
     1981 ccgtcgccat gctcacgcgc gcgccgcggg ccacggacgg ccacagcggc gtctcgaagg
     2041 tcgccagggg gacctcaacc tcgccggtcg cttccggccc ggccagccgg atcgggccca
     2101 cccagcgcag gggaatgctc gccgcgtccg tcgggttgtc aacgctcatg gccccagtgc
     2161 ataccttctt cccgggcgca aacaagaaaa aatcattttg ggcttctccc gccgggccgc
     2221 gaatcctact atggccgtca gggcggttgc atatccgggc ttaacctcaa cgggaggttg
     2281 ctgtgaaacg catgccttgg cggtccttgc tgctcgcgat cgtttctctc tccctcgcgg
     2341 ccgcgccgtc ggacggcgcc tcgaaaaaga agaaggccgc ggattcccag ccggcggtcg
     2401 aaaagaaagc cgcccggaag aagacatccc gcgcgaaaca ggagaagaag aacgagccgg
     2461 ccgtggaaac gcagcaaggg gcggccgcgc ttccccccgc cgaactcacg gcgggcgtcc
     2521 agttccgcga cgacgaggcg gaagggctcg gcgacatcct ggtccccgcg tggcggtttc
     2581 cctcgggact gctcttcgtc aatccccggg cgacgcggac ggattcggac gccgaggagt
     2641 acaatgtcgg gctcggctac cgccacctgt tcccgggacg cgcgatcatc ctgggcgcca
     2701 acgggtacta cgactaccgc gacacggggc acagcacata cgaccagtgg ggcgccggcg
     2761 tggaactgtt gagccggtgg ctcgacgccc gcgccaacgg ctactggccg gaagacacgc
     2821 gccacacggt ggccgaggcc tcggagacca gctccagccg gtccacgcgc accgaggaag
     2881 gctggaccga tccgtacgcg gccggccacg agatcgcgca gtcctacgtc gtcgagcaga
     2941 ccacccgcac gacgaccacg acccgctact acgagcagta tgagcaggcg ctcgacggct
     3001 gggacgccga ggtcgggctg cggctccccc tccccgtgcg gccggaggtg ctcgagcccc
     3061 gcgtcttcgg cggctactac cggtacgacg cggagtacgg ggaggacatc gagggcttca
     3121 aggcccgcgt ggaactgcgg ctgctcgcct cggtcttcct ggacgcggcg tggtacgagg
     3181 acgaggacct gacgggcagc gactactacg tcggcggccg gttcaccgtg ccgctggacc
     3241 tcgcggcggc tgcgcacggg cgcaacccgt tcgcgccgct ccgctcccgg ctccgcggcg
     3301 aggcgcgcgg cttcgacgcc cggctgaccg agatggtgat gcgcgatccg cagatccggc
     3361 tggagacctc ggagttcatc gagcgcgccg acctcgcgcg cgtggaccgg cggaagcaca
     3421 cggaaacccg gcaggaggac tacacgctga tggccgacgt gaacttcgtc agcggagacg
     3481 cgggttcccg cggcggagac ggcacggcgg agaacccgtt ccgcgccatc cagcccggcg
     3541 tggactcggc gttcggcgaa cgcaacgtct atgtctacaa cagctcgcgc gcctaccgcg
     3601 agaacgtcga actgaccccg ggcgtgaccc tgtggggcag cggctgcctg gtccccggct
     3661 acaacggcca gtccttcggc agcggcatcc agccgaccgt ggacggccac ggcggcgggc
     3721 cggcgatcag catggccaac cgcacgacga tccgcggctt ccggatcacc aatacgggcc
     3781 gcgggggaag ccgggtcgag gacctgcccg tcttcgggcc cacggacgtc agcggcgtcg
     3841 gcgtgctggg ccgggatgcc acggacctgc gggttgaatg caacaccatc gcgggcgcgg
     3901 acgtgggcgc gctcttcgcg cggcagggcg atttcagcct ggccttttta gataacgacg
     3961 tgctcgacaa ccgcgatgcc ggcctgatga tcggcggcca gggtagcagc gggaccttcg
     4021 acgtgctcgt ggacgggagc cggttcgagc gcaatggcat cgggctgatg agcgtggcct
     4081 ggaactacga cgaggccttc ctcaccatcc ggaacagcgc cttcgagcgc aacggggcgg
     4141 ccggggcttc gatcctgatg gaccagaacg gattcagttc cgtcgagatc gcggattccc
     4201 aggccgagcg caacgccggt ccgggattcg acatcaacat caccgcctcc gacgaagcgc
     4261 tcgtggcgct gtccggggtc ggctcccacc acaacggcgg ggccggcgtc caggttctga
     4321 cgctgggcac gggccaggcc gtggcaacca tcgagaacgt ccaggcgcac gggaacgcgg
     4381 gacccggcat cgatctggcc ttcctggaca acggggaggt gatggccagc gtcaaccgcg
     4441 cccacgcgga cggcaacgcg gggcccggga tccggatcac cgcgacggcg gccgacaccg
     4501 tgctggccag cgtggcgaac tcgtgggccg cggccaacgg gggcggcggc gtggagatcc
     4561 tcgtggacga cacggccatg gccctggcgc tggtcggcgg ctcgcaggcg cacgacaacg
     4621 cgggcggacc cgggttcaac gtcctgatcg aaaacaccga cgtggccctg gccaacctgt
     4681 ccgggctgac ggcgaacggc aacgagggcg gcggcatcct gctgaacatg caaagcccct
     4741 tcgcggccat cggggtgatc ggcctgcccg acgcgctcgc agggctcctg ggcggggtaa
     4801 tggaaacggc cggcggcttc ctgccggccg agacggcgat gctgctcggg catgccggcc
     4861 cggtcgaggc catcgggaac cagggcttgg gcgtcacggc gatcatcgac ggggacatga
     4921 tcgcgggcgg ggcgatattc gacatccatg ccgagaacaa cgtgggcgga ggggtcaggg
     4981 ggctcgtcac aagttccaac ggcttcgcgg tgggactcgg gggatcttcc gagaacatca
     5041 tggagatcgc gcagttgggc gccgatatcg cggagttctt cggcctcgat atccccgtca
     5101 tgccgtcgcc cggcgggcgg acggtcgtca ataacaacct cggcccggga atcggcctgg
     5161 gcgtcatggg caacatggcc gccgtcgggg ccctggtcgg cgtggcggcc gacggcaatc
     5221 tcgggctcgg cgcgggcgtg gccaccctgt ccgacggaat ttcggtgtcg gccgccgcgc
     5281 gcatcctggc cctgaacacg acgggcgacg gcctggtcgt ggaggcggat ggccccacgg
     5341 cggcggtcgg cgttgtcgcc gacgtgcagg ccctcggcaa cgccggcaat gggatcatca
     5401 ccgacatcaa cagcgacgaa ggttacgccg cggcgctgtt tgcctccacc gacccgctcc
     5461 gcccggtcgc ggaactgctc ggcgagctgt tgataggtga gccgctgacc gtgcccggcc
     5521 agcccttcgg cccggtgatc gcgtcgggca acggggagga cggcatcgcg gcccgcgtga
     5581 acggccgtga agcaggcatc gggttgttcc tcgacaccca ggccaccggc aacgggggcg
     5641 acggcatgga cgtttcggtc caatcgtccg aaggctacgc cgtttcggcg ttcctctcga
     5701 gcgattacct ctttaatctc gcatcgggat tcttcgggcc gatcgattac gagccgctgg
     5761 gcgggatcat cgccagcgac aacgacgggc acggcatccg cctgtggcaa aacggcctgg
     5821 ccggcgcgta cgccgtgctg gcgggcctgc aggccaacga caacgaaaaa gacggcctga
     5881 acgcccgctc gaacagcgag gagggcgacg ccatgactgc ggtcatcgtc gccgacgcca
     5941 tggacaacgg cgggcagggg ttcttcacgg aaaacaacgc aggcctggac agcctggccg
     6001 ccttcgtcgg cgtctgggcc tccggcaacg agcgccaggg catccgcgcg gtcgggacca
     6061 gcgtcaacgc caatgcctac gcgatcctgg ccggcgtcga gaccgtggac aacggagccg
     6121 cgggcgcctc ggtcgaactg ggcgccacgg gggacgccgt ggcctgcctc acggatgtag
     6181 acagctgtct caacgaaggc cggggcgcgg ccgtcacgct gaacgccgca ggcaacgcag
     6241 tgctgtgggc gggtcccaac gccttgaacc agttcaacag ccaatactcc ccgctgtttg
     6301 gcgtgttcaa cctggcggcc ttcctgatcc cgcaggacgc cgtcacgctg gacaacaacg
     6361 ggggcgcggg cctgtacgcc gacctgcgca gcgcggccgg caacgtcgtg atggaactgg
     6421 aggacgcgcg ggcgatcggc aacgacaagg acggattcaa ccttcagatg gccgcggcga
     6481 acggctcgat ctcggctacg ctccggaatg ccgtggccgg cgaaaacggc gcgcgcggtt
     6541 tcgacgtgtg ggcggagggg ggcggcggca cgctggacct atacctggag catgccgagg
     6601 ccaccggcaa caccaacgac ggggcgcgaa tccgggcgaa ctattccggg ccggtcaacg
     6661 tgtacggcga acaggtcgtc gcgatcaaca acggcggggc gggcgcgcgg atggacatcc
     6721 tggccggggg gacgctcaac atggacttcg gcggcggggc gctgggcagt ccgggccagg
     6781 gccagttctt cggcaacggg tcgcgcgact tccgcaatgc cggcgccgga acggtcttcg
     6841 cggaacagaa ctggtggggc gtcgatcccc cgtcggccgg ccagttcttc ggcgccgtgg
     6901 attacacgcc gtggctgacc gagccgccgc cgtgataaga aagagtatag gtcctatacg
     6961 tcgcatagga cttattcgac ctatacttga cgacggactg gaacgtggta gtgttcttcg
     7021 aaatgagtgt tcatttcatc agcgccgcgc gcaccggaaa aggcccgaac gcctcttcct
     7081 cgctgttgct ctcccttagc ggcaacaccc gcgcggcggt gggcgcgagg tgttgctcct
     7141 gacccgaccc gctccacgga gcgccgaaat cccgccgcga aaaacgccgg cgggtttttt
     7201 tattggaacg cgccgggaga tcggtaggct gaacccgttt gaaaaccaaa ggaacgtgac
     7261 catgagtgcg gcaaagaaca gtgaaaagaa tcgcgtgatc atcttcgaca cgacgctgcg
     7321 cgacggcgag caatgccccg gcgccagcat gaacatccac gagaagctgg aggtcgcccg
     7381 gcagctggca aggctgaacg tggacgtcat cgaggccggc ttccccatcg cctcgcccgg
     7441 cgacttcgag gccgtcaagg ccgtcgccga gcaggtgaag ggcccgtata tcgcgggcct
     7501 ggcccggtgc atcaacaagg acatcgaacg ctgcgcggag gccgtcgcgc ccgccgggaa
     7561 gaaggcgtac atccacacct tcctggcgac gtcggcgatc caccggcagt tcaagctgaa
     7621 gaaggccaag cacgagatcg tcaagcaggc cgtcgcgagc gtgaagcacg cccggacgtt
     7681 ctgcgaccgg gtgcagttca gcccggaaga cgcctcgcgc acggagccgg agttcctggc
     7741 cgaggtctgc gaggcggtca ttgacgccgg cgcgaccacc gtgaacatcc cggacacggt
     7801 cggctacgcc gtgcccatcg agttcggccg gctgatcgcg tacctggtcg agcacgtgaa
     7861 gaacatccac cgggcgatca tccacgtcca ctgccacaac gacctggggc tggcggtcgc
     7921 caactcgctg gccgcggtgc gcaacggcgc gcgcggtgtc gagtgcacga tcaacggcct
     7981 gggcgagcgc gcgggcaact gctcgctgga ggaggtcgtg atggggctga aggtccgcgg
     8041 ggacgccttc ggccgcctgt ggacctccgt gaagacgcgg gagctgtacc gcaccagccg
     8101 cctggtcagc cagatgagcg gcatggtggt ccagcgcaac aaggccatcg tcggcgcgaa
     8161 cgccttcgcc cacgaggcgg gcatccacca ggacggaatc ctgaaagagc gcacgaccta
     8221 cgagatcatg cgacccgagg aggtcggcat ctccggcagc gacatggtcc tgggcaagca
     8281 ctccgggcgg cacgccctgt tcgtccacct ggggcggatg ggcttccagg tcgccgaaga
     8341 ccagaaggag tccgtctaca accagttcaa ggagctgtgc gacaagaaga agtacatcta
     8401 cgacgacgac ctgatcgcca tcatgcgcga gcggatcagc gacatcccgc aggtgtacac
     8461 gctggactac ttccacgtcg cgacggggac cagcacggtc ccgaccgcga ccgtccgcct
     8521 gaagaagggc gaggaggtct tccaggactc cgcctgcggc gacggaccgg tggacgccac
     8581 gctgaagacc atcgaccgga tcaccggcac gcccgggacg ctggaggact actcgttgca
     8641 ggccgtcacc cgcggcgagg acgccgtcgg cgaggtcagc gtcacggtcg cgttcggcaa
     8701 ggacatggtc tccgccaagg ccgccagcac ggacatcgtg gaggccagcg cgaaggccta
     8761 cctgaacgcg ctgaacagtc acctgttcgc ccagcagacg gcgaaggggc cgaagaagaa
     8821 ggccgccggc cgccggcagg gacggcagcc atagatatag gtccaatacg tcgtatagga
     8881 cctattcccc ccgggttttc caaccattgg gaaatccggg gctttttttt ccaatgattg
     8941 gaaactttta gtgtggtatt ttccgaacat tggaaagcct tcaccccgga ggacgccatg
     9001 agcacgctgc tggtcaaaaa cggcctgctg gtcacgctga acgaacacgg cgaggtgatc
     9061 gagaacggga gcgtctacgt cgaggacgcc cgcatcatgg aggtcgggca cttccacgaa
     9121 aaccggtaca aggccgacca ggtcctcgac gccggcggcc ggctggtcat gccggggctc
     9181 atcaacgcgc accaccacct ctattccacg ttcgcctgcg gcttctcgcc gccgggccag
     9241 cccgcggaga acttcaagga aatcctcgag cacctgtggt ggaagctgga cttcgccctg
     9301 accgaggagg acgtctacta ctcggccctg ctcccgctgg tcgactgcgt caagcacggc
     9361 gtgacgacca tcattgacca ccacgcctcg ccttcgtgcc gcgacggcag cctggacctc
     9421 atcgagaagg ccgtccgcga ggtcggcatc aacgcgagcc tctgctacga ggtctccgac
     9481 cgcaacgtcg agggcggcgg ggtcgaggag aacgagcggt tcatccggaa gtgcgcggcc
     9541 gcggcggacg accaggtcac cgccctcttc ggcctgcacg cctcgatgac gctcggcgcg
     9601 aagacgctgg agcgctgcgc cgcgatcggg cgcgcccacg gcgcggggtt ccatgtccac
     9661 gtcgccgagg acctgatcga ccaggaggtc gcgcagaagg agttcggcaa gcgctgcatg
     9721 gagcggttcc gcgacgccgg gatgaccggc ccgcgctcga tcttcgtcca cggcgtccac
     9781 ctcgacgacg gcgaactgga catcctggcc gagacccact cgatccacgt ccacaacccc
     9841 gagtcgaaca tgaacaacgc cgtcggcacg gcgcggctcg cgcggttgtg gaagcggggc
     9901 atcctgacgg ggctcgggac ggacggcatg gcctcgaaca tgattgccag cgcgcgggcg
     9961 gcgtacctgc tgcagcggca cctcgagcac gacccgcgcg tcgcgttcgg cgagtgctgc
    10021 gacatgctgc tcaagaacaa ccggaccatc tgcgagcggc tgttcaagaa caagcgcggc
    10081 gtgctcgccg ccggcgcgct cgccgacctg gcgatcttcg actacacgcc cttcacgccg
    10141 ctctcgggcg cgaacttcta cgggcacctc ctgttcggcc tgtaccagtc ccgcgtcagc
    10201 gcgaccgtcg cccgcggccg cgtgctgatg aaggacggcg tcctgcccca cctcgacgag
    10261 gccgccctgc gccacaaggc caaggacctg gccgcgaaga tgtggaagcg gatcaagtag
    10321 gcggggcgag ggcgcctgtc ttcatccgcg atcacggccg gtaggcgcgg acgcggaaga
    10381 tcaggccggt cggactcccg atggcgaagg cgccctggtt cattttgggc gtcggcgccg
    10441 aataccatgc gcacccttgc cagggcccgc cgggatcacc cgcccattcc agcacctgat
    10501 cggcctgggt gccgccggcc cactgaacgt ccacgccgcc gggggcgacc gttgaggcgg
    10561 tgatggacag gatccgaagg ttgtcatccc cccgcgtggg atcggtatcc gcgatccact
    10621 cctcgccgtc gggcgagtca tcgccgtcgg tgtcgggatc gttcgggtcc gtgcccatgt
    10681 ccgtctcgga cgtgtacgcg ccggtgtggg tctccacgac gtccggcatg cggtcgccgt
    10741 cgctgtcccg gaactcgtcg cagcccatgt ccggcgtgtt ggccagccgc gattcgccgt
    10801 ccgcgtcggt gtcgtcccag agccagtaca ggctgccgag cgcgccgccg gcgttgatgc
    10861 agggcgagcc gtggcgcagg tggtggttgg gatagaactc cgggtaggcg tcgatgttgc
    10921 cggcgcccgg ccagccgccg ccgatgtcgc tgtggcggac gaccagcgat ccgccggcca
    10981 ggccgttggt gctgatctcc cagctctgat taccccagac gatgctgttg gagaccgaga
    11041 gctggatgtc gttgtacagg cgcaacccgt cgccgaggtt gccggccagc gtgcagaaga
    11101 gcgccgttcc cgtactgggc cccgcccacg cgatcgccgc gccgggcagt ccgccccagc
    11161 cggcgggctg ggtgcccccg gtgatcagcg tatgcgcgac gtgggcctgc ccgccgacga
    11221 tccccaggcc ggaagcatac gtcggcgcgc tgttggaaat gaaggccgcg tggctgatga
    11281 tggtgtcgca gtagtccagg tgcatcgcgc cgccgttatg agccaggttg ccctggaagc
    11341 tcagcgacca gccttcggcg gttgtgtagt agggcgcctc gacgacatag ccgcggatca
    11401 cggcatggag accgtacagg gcgccgccct cgttggtcgc cgcgttgccg agcaggtgtc
    11461 cacccacgag gagcaggatg gtgttttcca cgaagatccc gccgccctcg tcggccgtgt
    11521 tgttctcgag gcgattgtgc agcagggaga caggggcgcc ctccgcgcgc agcgccccgc
    11581 catggaccgc gcggttggtt tcgaagacgg cgttgaacgc ggtgaaatcg ctgcccgccc
    11641 cgacgtagac ggcgccgccg ccccccccgt tcgtgctgac gctgttgccc gcgaagcggg
    11701 gcgtcgcgaa ggcatcgccc ggcggtccgt tggtgatcgt catcatggac gagaagagcg
    11761 ccacggcgcc gcccaggccg ctcgcgggcg ggcccatcgg gttcggcgcg gcgttgtgcc
    11821 ggaaatccat gcccgagacc tggagccacg aacccgtggc gtacaaaccg ccgccgttcc
    11881 ccccggccac gttcaaccac ccggtgaatc gcgtgagatc ggccgtggtg ttttcgaggt
    11941 agaatcctcc accgtggccc tccgtgccgc actggtc
//
LOCUS       DAOOTK010000104        11971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3555_length_11971_cov_5.128315, whole genome shotgun sequence.
ACCESSION   DAOOTK010000104 DAOOTK010000000
VERSION     DAOOTK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_11971_cov_5.128315"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PK634_10930"
     CDS             complement(<1..952)
                     /locus_tag="PK634_10930"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOW98290.1"
                     /translation="MIPHRHSRWFPVAGFLLAAAAARAALVTEVGVRVQGHGRVDTNA
                     VMAFSTLRPGEEYNAMAVGRDLRAMQQSGMFSSVTVEAEPGTGGVAVYFLVEGRSRIR
                     DMDIEGADDLGNRKVRSLLEIGPGDPVDDSLLAVRSQKVLETYRKKYWPDARLTWALA
                     PAEEPGWVNVRITVKEGRRATVKKIVFAGNDRVRSRELLKAMKQRRANWLSWITGAGT
                     YDPDLLETDRELLREVYRGRGYLDAGIGEPKISPLGRRGLRVDIDVSEGEPYRVGRVE
                     FSGITSAPPAEVESAVRLKEGDTASSTNIEKNAQALQDWYG"
     gene            complement(949..2364)
                     /gene="dnaB"
                     /locus_tag="PK634_10935"
     CDS             complement(949..2364)
                     /gene="dnaB"
                     /locus_tag="PK634_10935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOW98291.1"
                     /translation="MAAPAARTDRLPPYSEEAERGVLGSALLDAGRVVDLAVERGLRP
                     ESFYVPAHQALFELFLDMGHQGRPVDLLTVGDRLRDQGRLEEIGGSTFLDQLVDATPT
                     PAHAEYYIDIVRQKHLLRLIIDRAREAIDTTYGAEESADSILGKVEQSFFEIGETQRG
                     QVLPWPTLVKEAVEEIEKIYALKKGLTGISTGYTDLDRILLGLHPTDMIILAARPSMG
                     KTSLALNIVENIGLGEGPDHVARPVAVFSLEMSKEQLVRRMICSHAKVPAHKLSSGML
                     FAEQHGHLINAASVLNKANILLDDTAGLEVLELRSRARRMKRRFDIQMVVVDYLQMLN
                     FPQFANEGRQRETAAISGALKAMAKELKIPVLVLSQLSRAPETRSATAVPKLSDLRDS
                     GSLEQDADVVLLLRRPCKYPEDPDHADELLAIVEVAKHRNGPTGSVHLNFFQDYTRFE
                     SRAAGVDQEGGPGPGGGEGAS"
     gene            complement(2366..2815)
                     /gene="rplI"
                     /locus_tag="PK634_10940"
     CDS             complement(2366..2815)
                     /gene="rplI"
                     /locus_tag="PK634_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOW98292.1"
                     /translation="MSMELILTENVEGLGTEGAVVRVAEGYARNYLLPRKLAAPVTEA
                     ARRRLETRRKAREEQMKKDLEGARALVASIEQASCTLTVKTGEGGKLFGSVTAADIAA
                     ALKGQGLELDKRQIELAEPLRELGVFNVPVKVHPEVQATLKVWIVED"
     gene            complement(2834..3028)
                     /gene="rpsR"
                     /locus_tag="PK634_10945"
     CDS             complement(2834..3028)
                     /gene="rpsR"
                     /locus_tag="PK634_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOW98293.1"
                     /translation="MAPIRRARYLKSDDTVNFRDYELLRKFMTERGKIMPRRFTGATA
                     KQQRQIKLAIRRARVMGLLP"
     gene            complement(3107..3571)
                     /gene="ssb"
                     /locus_tag="PK634_10950"
     CDS             complement(3107..3571)
                     /gene="ssb"
                     /locus_tag="PK634_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOW98294.1"
                     /translation="MASLNKVFLAGNLTRDVEVRYTPTGTAVADLNMAINRTYTSGGE
                     QKEEVCFVNVVAWGRTAELCGEYLSKGSPILVEGILQYDTWQTDAGEKRSRLRVRADR
                     VQFLGRPKRGEMGDAPEGGPPPRGGRPAAPPPPADEPPAGPDEGADHENLPF"
     gene            complement(3558..3929)
                     /gene="rpsF"
                     /locus_tag="PK634_10955"
     CDS             complement(3558..3929)
                     /gene="rpsF"
                     /locus_tag="PK634_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOW98295.1"
                     /translation="MKKYEIMFIFPEALKDEALAAALTKAQEEIKRVGGEIENVTQLG
                     KRGFARAMKKKEAGYYVLAVVRLPADQIAPLQAKYRLDEGIFRVQVVEAPASGSSKIT
                     VPGEYGERSDRSDRGERDGQP"
     gene            complement(3940..4512)
                     /gene="pth"
                     /locus_tag="PK634_10960"
     CDS             complement(3940..4512)
                     /gene="pth"
                     /locus_tag="PK634_10960"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOW98296.1"
                     /translation="MKIVLGLGNPGRKYEHTRHNIGFDVLDGMAAEAGVRFRKKWLMP
                     LQSAEIEVGSETVLLVKPMTFMNRSGSALRPLVRRHGLAPEDVIVVLDDLDLEAGRLR
                     IRKRGGAGGHKGLQSVLATLGTENFPRVRLGIGPRPVGEDLTGYVLARFGAAEREAAG
                     RAVRQAGDAVRMMVEAGVDQAMNKFNSETK"
     gene            complement(4534..5334)
                     /locus_tag="PK634_10965"
     CDS             complement(4534..5334)
                     /locus_tag="PK634_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOW98297.1"
                     /translation="MKTLKVTVQPRQSRGTAQAGRLRRQGLLPAVVYGSGKPTRTIQL
                     NRHDFEQSLRGHAGENILMDLEIEGEKTIKVLLKDIQHHPVSGQMTHADFNEISMTEK
                     LRVELPIHLVGEPVGVSQQGGILEHLIRQVAVECLPTDIVERVDVDVSGLSIGQSLTV
                     ADVKLDPAKYTVLTSKEVAVATVAAPRAEEEVAPAEAVAGEGAAAEPEVITAKKEEGE
                     EGEEGEEKEGGKKGEGKKPEAKKEEAGKKPEAGKKPEAAKKEEGRKAK"
     gene            complement(5353..6279)
                     /locus_tag="PK634_10970"
     CDS             complement(5353..6279)
                     /locus_tag="PK634_10970"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOW98298.1"
                     /translation="MRIFSGNAHRALAEEIAAYVEQPLGDVNITRFPDGEIGVKIVDN
                     IRGQDVFIVQPTCNPPNENLMELLIMVDAARRASAARITAVMPFYGYARQDRKDQPRV
                     PITAKLVANLLVAAGTSRLLAMDFHAQQVQGFFDIPVDHLFAAPVIVKHIRAKQLPDL
                     VVVAPDPGGLKMAYAYSKMLGAGLAIVAKQRQSATEVEALNLVGDVAGRNALLVDDLC
                     TTAGTLCAAANLLKARGTKQVFAAVTHAVLADQAIARLKNSPIEFMVTTNSVPAKNWN
                     GFPVEALSVADLLGEAILRIHSNQSVTSLFNV"
     gene            complement(6392..6465)
                     /locus_tag="PK634_10975"
     tRNA            complement(6392..6465)
                     /locus_tag="PK634_10975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6431..6433),aa:Gln,seq:ttg)
     gene            complement(6749..9979)
                     /locus_tag="PK634_10980"
     CDS             complement(6749..9979)
                     /locus_tag="PK634_10980"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOW98299.1"
                     /translation="MTPLGDYLSELKRNLDKGIATEHTYRPFLERLLEAFGKDVDATN
                     EPRRIACGAPDFIVTRRKTPLGYVETKDIGTDLDEMARGRGPHGEQFVRYRDGLANWI
                     LTDYLDFRWFVGGEPRLALRVAEVDAKGKLRPLPDAEPKLAALLHAFYEQPALTIGTA
                     RDLAVRMAALTRILRDLIAATFRHEAEELEKSKLREAAAEYGAGKPRGPWLHAWLSAF
                     RETLIPDLTEADFADMFAQTLAYGLFAARIHTPANKEFTREMAAYRLPKTNPFLRKLF
                     HEIAGVDMPDTMAWAVDDLVDLLRHADMIEILRDFGKGKGKEDPIVHFYETFLAAYDP
                     KLRESRGVYYTPEPVVGWIVRSLDRLLQSKFGRPSGLADEKTLILDPAAGTATFLYSV
                     LNEVYARFAKQKGAWDGYVEKHLLNRLFGFELLMAPYAIAHLKLGMRLEETGYGFAGD
                     RRLGVFLTNTLEQAAHKSEQLYASWISEEADAASEVKSKAPIMVVLGNPPYANFGRMN
                     RNPWILKLLEDYKRDLNEKKLNLDDDFIKFLRFAQWRIEQTGHGLIGFITNNTYLDGI
                     THRRLRRSLMESFDELYVLNLHGSSKKKEKTPEGGKDENVFDITVGVSILLAVKKGGE
                     SGLAGARPSRAVLANRTVEGGAPATPDHTAGARVSHADLWGRREQKYEWLGANEVEST
                     KWTKLKPQEPYWFFVPKDTGAQEEYEKYIGLKSVFQVLGNGVKTDRDELFVDFDRDVL
                     LNRIRTFYSVDGIAEPFSSTYRVENSSSYDLLARRQNTKFSEENIRPFLYRPFDMRWL
                     YYLQGLTSRPAYEVMRHMIAGPNVALVCLRQSRRGEAGTFLAVKGLINKDAVSLYDIG
                     TAFPLFHYPDSNELQLGSPRPNFTPSFIAAVEGRLGMKLEVGPAGPAGRAARSANAPY
                     LTPESIFHYAYAIFHSPTYRSRYAEFLKIDFPRLPLTSDRELFFRLAGLGAELAGLHL
                     MESPKLEQFITSFPEKGDNVVEKVQFADGRVYINKAQYFDGVPESVWAFHVGGYQVCE
                     KWLKDRKGRALAYEDVRHYQKIVVALAETLRLMAEIDRAIPGWPIR"
     gene            complement(10051..10611)
                     /locus_tag="PK634_10985"
     CDS             complement(10051..10611)
                     /locus_tag="PK634_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98300.1"
                     /translation="MDDPRALLQAAEDVVSILQQHRIDAVIIGGVALAAHHYVRQTED
                     IDLGINADLPALRVVEAALRRAGYAAELREPDAADPLGGVINVTGPFGSLQIISFSGR
                     FPAVIEDALREATVVVEEGSRLKIAPLPHLVAMKLYAGGFKSKADIVELLNRNPDADL
                     KAIRSLCRRYQLTGFDELLPDIRAHP"
     gene            11153..>11971
                     /locus_tag="PK634_10990"
     CDS             11153..>11971
                     /locus_tag="PK634_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98301.1"
                     /translation="MKIHTFLFLSFSALAWSPARAAMDLAVTKSGNPPVPAIGDTVTY
                     TITATNVGDTAATSATVSDVLPSGVWYLNHSNGTYAVTNGAWSIGALDVGSSTSLLIQ
                     ARVLMPPGEACVITNPTPEAGDYLGYSVAGLGSDKFIVGACIDNAGGTYAGSVYLYNT
                     NGSVLVTITNPVPAPSNYFGCSVASLGSDKIIVGAMQDDTGADNAGSVYLYDMNGALL
                     VTITNPAPDADDMFGNSVAGLGADKIIVGARYDDAGAYDDDAGGVYLYDTNGALL"
BASE COUNT         1847 a         4067 c         3808 g         2249 t
ORIGIN      
        1 tgccgtacca gtcctggagc gcctgcgcgt tcttctcgat gttcgtcgac gaggccgtgt
       61 ccccctcctt caggcgcacg gcgctttcca cctccgccgg gggcgcggag gtgatccccg
      121 agaactccac ccggccgacc cggtacggct cgccctcgct cacgtcgatg tcgacccgca
      181 gtccgcgccg cccgaggggc gaaatcttcg gctcgccgat gcccgcgtcg aggtaccccc
      241 ggccccggta gacctcccgc agcagctcgc ggtccgtctc gagcaggtcg gggtcgtagg
      301 tgcccgcgcc ggtgatccac gacagccagt tggcccggcg ctgcttcatg gccttgagca
      361 gctcccggct ccgcacgcgg tcgttgccgg cgaagacgat cttcttgacc gtcgcgcggc
      421 ggccttcctt gacggtgatc cggacgttga cccagccggg ttcctcggcg ggcgcgaggg
      481 cccatgtcag gcgggcgtcg ggccagtatt tcttgcggta ggtctccagg accttctgcg
      541 agcgcacggc gagcagcgag tcgtccaccg ggtcgcccgg cccgatctcg agcagggagc
      601 gcaccttgcg gttgccgaga tcgtcggcgc cctcgatgtc catgtcccgg atgcgggagc
      661 ggccctcgac caggaagtac acggccacgc cgccggtccc gggctccgcc tcgacggtca
      721 cgctcgagaa catgccgctc tgctgcatcg cgcgcaggtc gcggccgacc gccatggcgt
      781 tgtattcctc cccgggccgg agcgtggaga aggccatcac cgcgttcgtg tccacccggc
      841 cgtgcccctg cacgcgcacg ccgacctcgg tcaccagggc ggcccgggcc gcggccgccg
      901 cgagcaggaa gcccgcgacc gggaaccaac gcgaatgcct gtgcggaatc atgaggcccc
      961 ctctcctccg cccgggccgg gaccgccttc ctggtccacg ccggccgcgc ggctctcgaa
     1021 acgcgtgtaa tcctggaaaa agttcagatg caccgacccg gtcgggccgt tgcggtgctt
     1081 ggcgacctcc acgatcgcca gcagctcgtc cgcgtggtcc gggtcctcgg gatacttgca
     1141 gggccgccgc agcagcagca cgacgtcggc gtcctgctcg agcgagcccg agtcgcgcag
     1201 gtccgaaagc ttgggcaccg ccgtggcgct gcgggtctcc ggcgcccggc tcaactggct
     1261 caagaccagc accggtatct tcagctcctt cgccatggcc ttgagtgccc cggagatcgc
     1321 cgccgtctcg cgctgccggc cctcgttggc aaactggggg aagttgagca tctgcaggta
     1381 atccaccacg accatctgga tgtcgaaccg ccgcttcatg cgccgcgccc gggagcggag
     1441 ctccaggacc tcgaggcccg cggtgtcgtc cagcaggatg ttcgccttgt tcaggaccga
     1501 cgccgcgttg atcaggtgcc cgtgctgctc cgcgaacaac atgccgctgg acagcttgtg
     1561 cgccggcacc ttggcgtgcg agcagatcat cctccgcacc agctgctcct tcgacatttc
     1621 caggctgaag accgccacgg ggcgggccac gtggtcgggg ccctctccca gcccgatgtt
     1681 ctccacgatg ttcagcgcga gcgacgtctt gcccatcgac gggcgcgcgg ccaggatgat
     1741 catgtccgtc gggtgcagcc ccagcagaat ccggtcgaga tcggtgtacc ccgtggagat
     1801 gcccgtcagc ccctttttca gggcgtagat cttttcgatc tcctcgacgg cctccttcac
     1861 gagcgtgggc cacggcagca cctgcccgcg ctgggtctcg ccgatctcga agaaggattg
     1921 ctcgaccttg cccaggatgc tgtccgcgct ctcctccgcc ccgtaggtgg tgtcgatggc
     1981 ctcgcgcgcc cggtcgatga tcagccgcag caggtgtttc tgccggacga tgtcgatgta
     2041 gtattccgcg tgggccgggg tgggcgtggc gtcgacgagc tggtcgagga aggtcgagcc
     2101 gccgatctcc tccaggcggc cctggtcgcg cagccggtcg ccgacggtca gcaggtcgac
     2161 gggccgcccc tggtggccca tgtcgagaaa aagctcgaac agcgcctggt gcgccgggac
     2221 gtagaaggat tccgggcgca ggccccgctc gaccgccagg tccaccaccc ggccggcgtc
     2281 cagcagcgcc gaccccagga ccccccgctc ggcttcctcg ctgtacggcg ggaggcggtc
     2341 ggtcctggcg gcgggcgcag ccatgttaat cctcgacgat ccagaccttc agcgtggcct
     2401 gcacctcggg atgcaccttg acgggaacgt tgaagacgcc cagttcgcgc agcggctcgg
     2461 ccagctcgat ctgccgcttg tccagctcca ggccctggcc cttgagcgcc gccgcgatat
     2521 ccgcggcggt gaccgacccg aacagcttgc cgccctcgcc ggtcttcacc gtgagcgtgc
     2581 aggacgcctg ctcgatggag gcgaccagcg cgcgggcgcc ctccaggtcc ttcttcatct
     2641 gctcctcgcg cgccttccgg cgggtttcca gccggcggcg ggcggcttcc gtgaccgggg
     2701 cggccagctt ccgcggcagc aggtagttgc gggcatagcc ttccgccacg cgcaccacgg
     2761 cgccttccgt cccgagtccc tcgacgtttt ccgtcagaat caattccatg gacatgtggt
     2821 tctccctttc cgttcagggc agcaggccca tgacgcgggc ccggcggatc gccagcttga
     2881 tctgccgctg ctgcttggcc gtcgcgcccg tgaagcggcg cggcatgatc ttgccgcgct
     2941 cggtcatgaa cttgcggagc agttcgtaat cgcggaagtt gaccgtgtcg tcggatttaa
     3001 ggtagcgcgc ccggcggatc ggggccatgg cgctgcgggc cggcttgcgg gtttccttgt
     3061 cgctcgatgc tcgtcttttc attcgcgtgt cctttttatc gtctctctag aaaggaaggt
     3121 tctcgtggtc cgcgccctcg tccggccccg cggggggctc gtccgccggg ggcggcgggg
     3181 cggcggggcg gccgccgcga ggcggcgggc cgccttcggg cgcgtcaccc atctcgccgc
     3241 gcttcggccg gcccaggaac tgcacccggt ccgcccgcac gcgcaggcgg ctgcgcttct
     3301 cgccggcgtc ggtctgccag gtgtcgtact gcaggatgcc ctcgaccagg atcggcgagc
     3361 ccttgctcaa gtattccccg cacagctcgg ccgtgcggcc ccacgccacg acgttgacga
     3421 agcagacttc ctccttctgc tcgccgccgc tggtgtacgt ccggttgatg gccatgttca
     3481 ggtcggccac cgccgtgccc gtcggcgtgt agcgcacctc gacgtcccgg gtcaggttcc
     3541 cggccaggaa gaccttgtta aggctggcca tcgcgctctc cccggtcgct gcggtcgctg
     3601 cgctcgccgt actcgcccgg gacggtgatc ttgctgctgc cgctcgccgg cgcctcgacg
     3661 acctgcacgc ggaagatgcc ctcgtccagg cggtacttcg cctgcagggg cgcgatctgg
     3721 tccgcgggca gccggacgac cgccagcacg tagtagcccg cttccttctt cttcatcgcg
     3781 cgggcgaagc cccgcttgcc aagctgggtg acgttctcga tctcgccgcc cacccgcttg
     3841 atctcctcct gggccttcgt cagggcggcc gccagggcct cgtccttcag ggcctccggg
     3901 aagatgaaca tgatttcgta tttcttcaat ggcgtctcct catttcgttt cgctgttaaa
     3961 cttgttcatg gcctggtcca cgccggcctc gaccatcatc cgcaccgcgt cgcccgcctg
     4021 ccggaccgcc cgccccgccg cctcccgctc cgccgcgccg aagcgcgcga gcacgtagcc
     4081 cgtcaggtcc tcgcccaccg gccgcggccc gatgcccagc cgcacccgcg ggaagttctc
     4141 cgtccccagc gtcgcgagca ccgactgcag gcccttgtgc ccgcccgccc cgccgcgctt
     4201 gcggatgcgc aaccgccccg cctcgaggtc gagatcgtcc agcacgacga tcacgtcctc
     4261 cggggccagg ccgtggcgcc ggaccagggg gcgcagggcc gagccgctgc ggttcatgaa
     4321 cgtcatcggc ttcaccagca gcacggtctc ggaacccacc tcgatctcgg cggactgcag
     4381 cggcatgagc catttcttcc ggaaccggac cccggcctcg gccgccatcc cgtcgagcac
     4441 gtcgaacccg atgttgtgcc gggtgtgctc gtacttccgg cccggattgc cgagtcccaa
     4501 cacgatcttc accggacggc tccccccgcc gcgttacttg gccttcctgc cctcttcctt
     4561 cttggccgcc tcgggcttct tgccggcctc gggtttcttg ccggcctctt ccttcttggc
     4621 ttccggcttc ttgccctcgc ccttcttgcc gccttccttc tcctcgccct cctcgccttc
     4681 ctcgccctct tccttcttcg ccgtgatcac ctcgggctcc gcggcggcgc cctcgccggc
     4741 gaccgcttcc gccggggcga cttcctcctc ggccctcggc gcggccaccg tggccacggc
     4801 gacttccttc gaggtcagga ccgtgtactt cgccgggtcc agcttgacgt cggccacggt
     4861 caggctctgg ccgatactca agcccgagac gtccacgtcc acgcgttcca cgatgtccgt
     4921 cggcaagcac tcgaccgcga cctggcggat caggtgctcc aggatgccgc cctgctggct
     4981 cacgccgacc ggctcgccga ccaggtggat cggcagctcg acgcgcagct tctcggtcat
     5041 cgagatctcg ttgaagtccg cgtgcgtcat ctggcccgac acgggatggt gctggatatc
     5101 cttcagcagg accttgatcg tcttctcgcc ctcgatctcc aggtccatca ggatgttctc
     5161 gcccgcgtgg ccccggagcg actgctcgaa gtcgtggcgg ttcaactgga tcgtgcgcgt
     5221 cggcttgccg gacccgtata cgacggcggg cagcaggccc tgtcggcgga ggcggccggc
     5281 ctgggcggtg ccccggctct ggcgcggctg tacggtgact ttcaacgttt tcatcgctgt
     5341 ctcccttgct ggctatacgt taaagagcga agtgaccgat tggttgctgt ggatacgcag
     5401 gatggcctcg ccgagcaggt cggcgacgga gagggcttcc acgggaaatc cgttccagtt
     5461 cttggccggc acgctgttcg tggtcaccat gaactcgatc ggcgagttct tgaggcgcgc
     5521 gatggcctgg tccgcgagca cggcgtgcgt gacggccgcg aagacctgct tcgtgccgcg
     5581 cgccttcagc aggttggcgg cggcgcagag cgtgccggcc gtcgtgcaca ggtcgtccac
     5641 gagcagcgcg ttgcggccgg cgacgtcgcc gaccaggttc agcgcctcga cctccgtcgc
     5701 gctctggcgt tgcttggcga cgatcgccag gcccgccccc agcatcttgg agtaggcgta
     5761 ggccatcttc agcccgcccg ggtccggcgc cacgacgacc agatcgggga gttgcttcgc
     5821 gcggatgtgc ttgacgatca ccggcgccgc gaagagatgg tccaccggga tgtcgaaaaa
     5881 gccctgcacc tgctgcgcgt ggaaatccat ggccagcagc cggctcgtcc cggccgcgac
     5941 gagcaggttg gcgaccagct tcgccgtgat cggcacccgc ggctggtcct tgcggtcctg
     6001 ccgggcgtac ccgtagaacg gcatcacggc cgtgatccgc gccgccgacg cgcgccgggc
     6061 cgcgtcgacc atgatcagca gctccatcag gttttcgttc ggcggattgc acgtgggctg
     6121 gacgatgaac acgtcctgcc cgcggatgtt gtccacgatc ttgacgccga tctcgccgtc
     6181 cgggaagcgc gtgatgttca cgtccccgag tggttgctcc acgtacgcgg cgatttcctc
     6241 cgccagcgcg cggtgcgcat tgcctgaaaa tattctcata tccacttcaa ttacccctgc
     6301 ctggcttgcc agccgtagct cggagcagca tcgcaactaa atgcccgcgc tatcgtccgc
     6361 cttcgccctc cgcttcgctc cgagcgaagg ctggttgccc gaccaggact cgaacctgga
     6421 ctctggcctc caaagggccg tgtgctgcca ttacaccatc gggcatcccg ccgcggccca
     6481 aaaaaatccg gcggcgcccg gctcactgac ccctcgaggc cgccgtcggg ttcccgcctc
     6541 tatataagaa atacggccct tatcccgcga tcccgtccct ccgagccgta acaagtgcga
     6601 cagagtatgg accgccccgt gaaaagtcaa ggttgggata agaaaatcgg ggcatcccag
     6661 tcggcgcccc cccatccacg tccatccgcg tgtatctgcg gttcttttct tgccgcggat
     6721 ttcacggagg ggcgcggatc aagccttttt accggattgg ccagcccggg atggcccggt
     6781 cgatctcggc catcagccgc agggtctcgg ccagggcgac cacgatcttc tggtagtgcc
     6841 gcacgtcctc gtaggccagc gcccggccct tccggtcctt gagccatttc tcgcagacct
     6901 ggtagccgcc gacgtggaag gcccagaccg actccggcac gccgtcgaaa tactgggcct
     6961 tgttgatgta gacgcggccg tccgcgaact ggaccttctc caccacgttg tctcccttct
     7021 cggggaacga ggtgatgaat tgctccagct tcggcgattc catgagatgc aggcccgcca
     7081 gttccgcccc cagccccgcc agccggaaga acaactcccg gtccgacgtc agcggcagcc
     7141 gcgggaaatc aatcttcagg aattccgcgt accggctccg gtatgtcggc gaatggaaga
     7201 tcgcgtaggc gtaatggaag atggattcag gggtcaggta gggcgcgttc gccgaacgcg
     7261 ccgcgcggcc ggctgggcca gccggcccta cctccaactt catccccaac cttccctcca
     7321 ccgcggcaat gaaggacggg gtgaagttgg ggcggggaga gcctagttgt aactcgttgg
     7381 aatcaggata atggaagagc ggaaaagctg tgccaatgtc gtacagcgaa acagcgtctt
     7441 tgttaatcaa tcctttaacc gccagaaacg ttccggcttc gcctcggcgg gattgccgca
     7501 agcaaaccag agccacattc ggtcccgcaa tcatgtgccg catgacttcg taagccggcc
     7561 tgcttgtaag cccctgcaga tagtacaacc aacgcatatc gaaaggacgg tagagaaatg
     7621 ggcgaatgtt ttcttcgctg aattttgtat tttgccttcg agccaaaagg tcataactcg
     7681 aagaattctc aactcgatac gttgacgaaa agggctccgc aatcccatcc acggagtaga
     7741 aagtacggat tctgttcagc agaacatcac ggtcaaaatc aacaaacagc tcatcgcgat
     7801 cggttttcac tccgtttcca agaacttgaa acacgctctt caagcctatg tatttctcat
     7861 actcctcctg cgcccccgta tccttcggca cgaagaacca gtagggctcc tggggtttga
     7921 gtttcgtcca tttcgtggat tcgacttcgt ttgcgccgag ccattcgtat ttctgttccc
     7981 gccggcccca caggtcggcg tgggagaccc gggcgccggc ggtatgatcc ggcgtcgcgg
     8041 gagctccgcc ctccacggtc cggttcgcaa ggacagccct ggagggtcga gctcccgcga
     8101 gaccgctttc cccacccttc ttcaccgcca atagaatgga cacgccgacg gtgatgtcga
     8161 agacgttctc gtccttgccg ccctcgggcg tcttttcctt tttcttcgag ctgccgtgga
     8221 ggttcaggac gtacagctcg tcgaaggact ccatcagcga ccgccgcagc cggcggtgcg
     8281 tgatgccgtc gagatacgtg ttgttggtga tgaacccgat caggccgtgg cccgtctgct
     8341 cgatgcgcca ctgggcgaac cgcaggaatt tgatgaagtc gtcgtcgagg ttgagcttct
     8401 tctcgttcaa gtccctcttg tagtcctcca gcagtttcag gatccacggg ttgcggttca
     8461 tccgcccgaa gttggcgtag ggcgggttgc cgagcacgac catgatcggc gccttcgact
     8521 tcacctccga ggcggcatcg gcctcctcgg aaatccagct cgcgtaaagc tgctcgctct
     8581 tgtgggcggc ctgttcgagg gtgttggtga ggaagacgcc gagccgccgg tcgccggcga
     8641 agccgtagcc ggtctcctcc agccgcatgc caagcttgag gtgcgcgatg gcgtacggcg
     8701 ccatgagcag ctcgaagcca aagaggcggt tcaggagatg cttctccacg tagccgtccc
     8761 acgcgccctt ctgcttcgcg aaccgggcgt acacctcgtt gagcacggaa tacaggaacg
     8821 tcgccgtgcc ggccgcagga tcgaggatca gggtcttctc gtccgccaag ccgctgggcc
     8881 ggccgaactt cgactggagc aggcggtcga gcgagcggac gatccagccg acgacgggct
     8941 cgggggtgta gtagacgccg cggctctcgc gcagtttcgg gtcgtaggcg gcgaggaagg
     9001 tctcgtagaa gtggacgatc gggtcttcct tgcccttccc cttcccgaag tcccgcagga
     9061 tttcgatcat gtccgcgtgg cgcagcaggt ccacgaggtc gtccaccgcc cacgccatgg
     9121 tgtcgggcat gtccacgccg gcgatctcgt ggaacagctt gcggaggaag ggattggttt
     9181 tcggcagccg gtaggccgcc atctcgcggg tgaactcctt gttcgccggg gtgtggatgc
     9241 gcgcggcgaa gagcccgtag gccaaggtct gggcgaacat gtcggcgaag tcggcctccg
     9301 tgaggtccgg gatcagcgtc tcgcggaagg cggagagcca ggcgtggagc caggggccgc
     9361 ggggtttccc ggcgccatac tcggcggcgg cctcgcgcaa cttcgatttt tccagttcct
     9421 ccgcctcgtg gcggaaggtg gccgcgatga ggtcccgcag gatgcgggtc agggcggcca
     9481 tgcggacggc gaggtcgcgg gcggtgccga tggtcagagc gggctgctcg tagaaggcat
     9541 ggagcaaggc ggccagtttc ggttccgcgt ccgggagcgg ccgcagtttc cctttcgcgt
     9601 ccacctccgc cacgcgcagg gccaggcgcg gctcgccgcc gacgaaccag cggaagtcga
     9661 ggtaatccgt gagaatccag ttggccagcc cgtcgcggta gcggacgaac tgctcgccgt
     9721 gcgggccgcg gccccgggcc atctcgtcga ggtccgtccc gatatccttg gtctcgacgt
     9781 agcccagcgg ggttttccgg cgcgtgacga tgaagtccgg ggcgccgcag gcgatgcggc
     9841 gcggctcgtt ggtggcgtcc acgtccttgc cgaaggcttc gaggagccgt tcgaggaacg
     9901 ggcggtaggt gtgctccgtg gcgatcccct tgtcgaggtt ccgcttgagt tcagagagat
     9961 agtcccccaa cggcgtcacg gagggaattt aacaaacctc cccatccgcg tccatccgcg
    10021 ttatccgcgg ttcctttctt accgcggatt tcacggatgg gcgcggatat ccgggagcaa
    10081 ctcgtcaaag ccggtcaatt gatatcgtcg gcacagggaa cgaatcgcct tcagatccgc
    10141 atccggattc cggttcaaca attccacaat atcggccttg gacttgaatc ctccggcata
    10201 gagtttcatc gccaccaggt gtggcagcgg cgcgatcttg agccggctgc cctcttcgac
    10261 gacaaccgtg gcctcgcgca gggcgtcttc gatgaccgcc gggaaacggc ccgaaaaact
    10321 gatgatctgc agcgatccga aagggcctgt cacattgatc acgccaccga gcggatccgc
    10381 ggcgtccggc tcccgcaatt ccgctgcata acccgcccgg cgcaaggcgg cctccacaac
    10441 gcgcagcgcg ggcagatcgg cgttgattcc aagatcaata tcctccgtct ggcgcacata
    10501 gtggtgtgcc gccaaggcca cgccgccaat gatcaccgcg tctatccgat gctgttggag
    10561 aatggaaaca acatcttccg cggcctgaag tagcgctcgg ggatcatcca cggcgtggcg
    10621 aggctcccac gggtttgagc catgagaagc gcctgctcat ggtgagcgcg gcccggatcc
    10681 tgtcaaggac cggcataccg cgcaggcgct cgacttcccg ggcgcgcaat ccctccgcac
    10741 acacgctgcg ccgaggcccc gaaagcccgc caaggcgttt tccctgactc gtcatgctct
    10801 tgaaactact cttgcagggt gaagattgca aatgcccaat tcttcaactt ccttgagtaa
    10861 cttaccctta caacggcggt cggaaaacta tgccggctgc gggtccacca gccataggcg
    10921 gggtttccat ccgcgtattt ccgcggttct ttttttgccg cggatttcgc ggaggggcgc
    10981 ggataggggg cctggcggcc cggctccaac ataaagcgcg ccagttggtt atcgttattc
    11041 gcgcctcgct tttttcttga ttcctgcggg gggcgcagga agaatgcgcg ccaccattgt
    11101 tggtcgtgga tgggcgcgga gaactgcggc aacacaagga ggacggcggg ccatgaagat
    11161 ccataccttt ctattcctgt ctttttcagc gttggcatgg tcgcctgcgc gggcggcgat
    11221 ggacctggcg gtgacgaaga gcggaaatcc acccgtgccg gcgatcgggg acacggtgac
    11281 gtacacgatc acggcgacga acgtcggcga cacggcggcg acgtcggcca ccgtttcgga
    11341 cgtgctgccc tcgggggtct ggtatctcaa ccacagcaac gggacgtatg cggtgacgaa
    11401 cggggcgtgg agcatcggcg cgctcgatgt gggttcgagc acatcgctgc tgatccaggc
    11461 gcgcgtactg atgccgcccg gcgaggcctg cgtgatcacc aaccccacgc cggaagcggg
    11521 cgattactta ggctattccg tggcgggcct gggctcggac aagttcatcg taggcgcctg
    11581 tattgacaat gccgggggga cctatgccgg gagcgtgtat ctctacaaca cgaacgggag
    11641 cgtgctggtg acgatcacca atcccgtgcc ggcgccaagc aattacttcg gctgttcggt
    11701 ggcgagcctg ggctcggaca aaatcatcgt gggcgccatg caggacgaca ccggcgcgga
    11761 caatgccggg agcgtgtatc tctacgacat gaacggggcc ttgctggtga cgatcaccaa
    11821 tcccgcgccg gacgcggacg atatgttcgg caattccgtg gcgggcctgg gcgcggacaa
    11881 gatcatcgtg ggtgcaaggt atgacgacgc cggagcgtat gatgatgatg ccgggggcgt
    11941 ctatctctac gacacgaacg gggccttgct g
//
LOCUS       DAOOTK010000105        11857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3599_length_11857_cov_4.963650, whole genome shotgun sequence.
ACCESSION   DAOOTK010000105 DAOOTK010000000
VERSION     DAOOTK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3599_length_11857_cov_4.963650"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PK634_10995"
     CDS             complement(<1..1292)
                     /locus_tag="PK634_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOW98302.1"
                     /translation="MTTAAPSKMAVLLERTGLLSPPQIEELLALYGQGGQSLPGLVVS
                     KGYAREEAFLEKLAEVLGVPFLRVGEQPIPADVLTKLPPKAVFQYNVVPVALENNVLR
                     VATPDPFLPGLVDSLRLASGLRVRLALSPAADLAKAAKKLYGVGADTMEQMIQDNRIE
                     VAPEEDILAKQDLSELDQEASVVKFVNQVIWEAYQDRGTDIHLEPMETDLRIRYRIDG
                     VLHQTPVPPQLKRFQAAIISRIKVMAGMDIAEKRLPQDGRISLRIRGEEIDVRVSTMP
                     TVYGESVSLRLLMRSSGMLGMDKLGLSEKDEAVLKKLIHKPHGILLVTGPTGSGKSTS
                     LYAWLHTINSIDKRILTIEEPIEYEMAGINQIQVRPEIGLTFAVGLRHILRQDPDVIM
                     VGEIRDKETAEIAIRAALTGHLVFSTLHTNDAAGAVTRL"
     gene            complement(1554..3104)
                     /locus_tag="PK634_11000"
     CDS             complement(1554..3104)
                     /locus_tag="PK634_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98303.1"
                     /translation="MTLRLNHPVVPVLLLALVAVLPYTRGLAYPFVYDDLGSIAENSF
                     LQEPGAWGRVLSGRTLTDPAVPDGRRPAVVATYLLDRALWGLRPAGYHAGNLLLHAGC
                     ALLFYGLLRRLTGGRFIPFGAALLFALHPVNVEAVQSPAFREDLLVGLFGLAFLLAAC
                     RRDAWGWLAVPLLALALLSKESAVAFVPLLGWVWLCFPEARPGRARMAGMGGAAAALA
                     LGAAVLWWASGSVQALRGGPDGVGLAFPRNLWTAPALWLMALRRLALPWPLLVDHVVD
                     PVDSWLALPFLAGWAVWAGWIAAAFALRRRAPDLALALGWMLLAFLPVSNVVPLFNPF
                     AERYLHLLAPGAGLLAAGLLARAPSPRTRAVLLAALAAAGAALILARLPDWSSNEALW
                     RKTLEQEPRSARAHTWVALDLKHRGAVGEARRLLEEADRLNPQDTSALINLAVLAGQE
                     GDLASAVERLREATRRRPDKADGWWNLAVALHQLGREEEAAEAVRRTLEADPRHPAAR
                     AVTAPAAE"
     gene            complement(3108..4148)
                     /locus_tag="PK634_11005"
     CDS             complement(3108..4148)
                     /locus_tag="PK634_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOW98304.1"
                     /translation="MAPLGKITDDFFQKIIKPRLGARRPEVAVGPEQGIDVGILELGE
                     YALAMTADPVFIVPEYGFERSAWFAIHILASDAVTSGLPPAFLSIDLNLPPELSDDDL
                     ARMWEVIHRECEKMGAAIVTGHTGRYDNCRYPMVGGATFMAMGPRDAYCSPKFCRPGD
                     RILITKGPAVEATGIFAAMFPDRIEKEFGAAVRKQAEDVFFRMSVVEDARLAASVGVR
                     DRGVAAMHDATEYGITGGLLEFAKAAGLGLVAHYDEIVMTPAVAEICRMFDIEPFSSI
                     SEGTLIAAVRPKAANEVLRRLQDQGILASICGEMTDAAEGIHAVTGGKKKPMEHPGED
                     PFWAAFFRALKG"
     regulatory      complement(4220..4327)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4381..5280)
                     /gene="era"
                     /locus_tag="PK634_11010"
     CDS             complement(4381..5280)
                     /gene="era"
                     /locus_tag="PK634_11010"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOW98305.1"
                     /translation="MPPAGTPELKPSALVAVVGRANAGKSTLVNALVGEKVSIVSPVA
                     QTTRALVRAILTEPRGQLVFLDTPGVHRATYDLGKVMNRTARASVEGADMVLLVVDAA
                     QPPREEDLGWIRRLGKEAVPVVAALNQCDRNTDHAAAFRAAWTGPAVPVWVEVSGLTG
                     QGTGALLEALFRLAPPGPPLFPDDVVTDYPRKLIIADTVREKYFQVLKEELPHALAVE
                     IESLEEGPGGWIAEGTVFVQKNSQKGIVLGAKGRLLKRVQAAAEKELADVYGRPVTLS
                     LWVKVDPNWSRNYWMLKRLGYAE"
     gene            complement(5290..6675)
                     /locus_tag="PK634_11015"
     CDS             complement(5290..6675)
                     /locus_tag="PK634_11015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOW98306.1"
                     /translation="MRIQFVGGVDTVTGTQHIVECGGVRVLRDCGLYQGHRKEAREIN
                     SRLPFDPSRLDSVVLSHAHIDHCGNLPSLARAGFRGPIHATTATAALCGVMLRDSAHI
                     QEQDAAYLNQKTSRKDEEPIVPLYTLRDAEAAIALFRGYRYGEPVDLGAGLQATFRDA
                     GHILGAALTVFTERKDGAEKRAGFAVDLGRDDLPLIRDPEPMEGLDALVLESTYGDRL
                     HGEAGAAEDQLHAVVERTLRRGGKVMIPSFALERTQEVIFHLASLVQAGRLSNIRVYV
                     DSPMATAISGLFEKYAEYLDDEYRNLNHRFGSVMYPEWVRYIASVEESKAVTACPDSC
                     IIIAASGMCEHGRILHHLKHGIENPKNAVVMVGYQASYTLGRRLLNGEKKVRIFGDWF
                     EPKAEVASLDAFSAHADRNELVEYVRQTRPRRIFLVHGEPDQREALAAALREMTKARV
                     ELPKPGDTAEI"
     gene            6852..7541
                     /locus_tag="PK634_11020"
     CDS             6852..7541
                     /locus_tag="PK634_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOW98307.1"
                     /translation="MKKAAGRTGTRRRAPAKKLYRALFNAWGPQRWWPGRTRLEVIVG
                     AVLTQNTAWGNVEKAIGRLRRADALSLPRLHALPLRQLEGLIRPAGYFRVKARRLRAL
                     TTMIVDRHGALPALFRLSTPDLRDELLRVNGVGPETADSILLYAAGRPAFVVDAYTRR
                     FMTRHGWLAPKATYDETARALAGGLPRNVRLYNEFHALIVRLGKDCCRPRPLCGGCPL
                     APWLPRGGPRP"
     gene            7538..7894
                     /locus_tag="PK634_11025"
     CDS             7538..7894
                     /locus_tag="PK634_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98308.1"
                     /translation="MRLEPALGLALALFTLGCTSPVTTAPAPAPAAPGFVGRVVRVNA
                     AQRFVIVQCERLPSEGDTLTLYRGKEPAGRLRATGPFRPPYVAADVLEGAPERGDAVR
                     AEARITAEPETSGSNL"
     gene            7891..8097
                     /locus_tag="PK634_11030"
     CDS             7891..8097
                     /locus_tag="PK634_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOW98309.1"
                     /translation="MNLAFLNRLSAWEIILILAVVLLLFGAKKLPELARSLGRSLQEF
                     KKGQQEGASDKPDDEGKPGGPPKA"
     gene            complement(8116..9279)
                     /locus_tag="PK634_11035"
     CDS             complement(8116..9279)
                     /locus_tag="PK634_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98310.1"
                     /translation="MEPTGDIYQKADRPYHVSRRSRFLLRHGRMPSASQSPPAAGADP
                     ATPGEAPPAGPAPSSHYGRRRRKSGSRRFHWRYFLSSRLNSALIAFSLVVGGLMLLVV
                     LTNFMKYRIQIQREAREADAPPATPPPASSNAIPSDLGARIAAWRQMPDRLAEVEGLR
                     QKSLAGEAEQRLEQALKDYPSAVAVRLTLARVLADAGRPAEAVVQLREALDAQPADPE
                     ARLLLGAALGKLGEYDLCRQVAEWMVETDPYSMEAHRLAATSYLKTDQPRAAIPHLRK
                     IVNIEFDNLDVQSELADALSRAGEHDKALATLEGVLNEDPANAMALFTRAVCYVRQNR
                     TDEACETLRRAAGHVGTDAMADWMGRPEWELLRELPWDSAEPPALTPDGATGE"
     gene            complement(9333..10520)
                     /locus_tag="PK634_11040"
     CDS             complement(9333..10520)
                     /locus_tag="PK634_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98311.1"
                     /translation="MSGARGQCGPLRKDRRLLLLIPALCVLAGCAGPGPDRDFRLAAR
                     REPERAAWVPKVPPVSDFKSCGEQAPLAAVLRFWGRPVRPGSLAEPGADPAELPDSEE
                     AVVRTVWRQGLWAYPCSASDERLKEWLRAGLPVLVRLEEAADGASLVDALVVGFDDTK
                     SLRLVDRVPGGRAGQPSADFLRRWRAADHRALLIRPPDDARLELGAEERMFRGRYFEA
                     AGQAAPAVRDYEAALEAGARSPAMLVSLGNLYRGLDRAGEAEELYRRAIREDPRQARA
                     YNNLAYLLAEQNRDLDEAARLARQALVLDPANPLFMDTLGYALMEHKQYDEAARVLER
                     AWGRSRWYPTHTQMEIGLRLALAHFRGGQEHLASEVLRDLLSMDPSLALPEELRPLAP
                     ADR"
     gene            complement(10520..10825)
                     /locus_tag="PK634_11045"
     CDS             complement(10520..10825)
                     /locus_tag="PK634_11045"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOW98312.1"
                     /translation="MKASKMLILLGMAVVLGAGGCATTPGPADDKALADERLTQEVER
                     RLEEDPVTAPFSFGVFVRDGVVIMEGAISSSYVRSRAIGIARATPGVQNVLDKLAPW"
     gene            10980..11771
                     /locus_tag="PK634_11050"
     CDS             10980..11771
                     /locus_tag="PK634_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOW98313.1"
                     /translation="MKKTVILILLAGWTLAAGAADPVIPDLYERQVLPNLDSSWISGI
                     TYYLEKGGDSGMLVVELKGQKITYLDVPLALWQEFQQTDSPGSLYGKKVKNKFERVEG
                     DPLWMRYDAAPPETLTGQVTCSFNEECETVVVQALGEARRTVRVAAYAFTRSRIARAM
                     VEARERGVDIQIKMDSQQAEYPLAVRTIEWLRNKGIPVTLITMSGDYAAMHNKFIVID
                     EQVVLTGSFNFTTTAVTANWENMLRVDSPEIAARFLAYWEVIASN"
BASE COUNT         1855 a         4163 c         4076 g         1763 t
ORIGIN      
        1 aggcgggtga cggccccggc cgcgtcgttc gtgtgcagcg tgctgaagac caggtggccg
       61 gtcagggcgg cgcggatcgc gatctcggcg gtctccttgt cgcggatttc gccgaccatg
      121 atcacgtccg ggtcctggcg caggatatgc cgcagcccga ccgcgaacgt caggccgatc
      181 tcgggccgca cctggatctg gttgatcccg gccatctcgt actcgatcgg ctcctcgatg
      241 gtcaggatgc gcttgtcgat cgagttgatc gtgtgcagcc aggcgtacag cgaagtggac
      301 ttgcccgagc ccgtgggccc ggtgaccagc aggatgccgt ggggcttgtg gatcagcttc
      361 ttgagcaccg cctcgtcctt ctcgctcaag cccagcttgt ccatgccgag catgccgctg
      421 gagcgcatga gcaggcggag gctgacgctc tcgccgtaga cggtgggcat ggtggacacg
      481 cgcacgtcga tttcctcgcc gcggatacgc aggctgatgc ggccgtcctg cggcaggcgc
      541 ttctcggcga tgtccatgcc ggccatgacc ttgatgcgcg agatgatcgc ggcctggaag
      601 cgcttcaact ggggcgggac cggcgtctgg tgcagcacgc cgtcgatgcg gtagcggatg
      661 cgcaggtcgg tctccatcgg ctcgaggtgg atgtccgtgc cgcggtcctg gtaggcctcc
      721 cagatgacct ggttgacgaa cttgacgacc gaggcctcct ggtcgagttc gctcaagtcc
      781 tgcttggcca ggatgtcctc ttccggcgcg acctcgatcc ggttgtcctg gatcatctgc
      841 tccatcgtgt cggcgccgac gccgtagagc ttcttggccg ccttggcgag gtccgccgcc
      901 gggctcaagg ccaggcgcac ccgcagcccc gaggcgaggc gcaggctgtc caccaggccg
      961 ggcaggaagg gatcgggcgt ggccacgcgc aggacgttgt tctccagcgc caccggcacg
     1021 acgttgtact ggaacacggc cttcgggggg agcttggtca gcacgtccgc cgggatgggc
     1081 tgttcgccca cgcgcaggaa cggcacgccc agcacctccg ccagcttttc caggaacgcc
     1141 tcctcgcgcg cgtacccctt gctcaccacg aggcccggca gggactgccc gccctgcccg
     1201 tagagcgcca ggagttcctc gatctgcggg ggcgagagca ggcccgtgcg ctcgaggagc
     1261 acggccatct tggatggggc cgctgtggtc atgcgttcat catgcacggt cgggcggcgg
     1321 gattcaaggg ccatcgggcc ctcgcgcatc cggccgccgc acccgtttag tccgccccat
     1381 gacccgccgc gttcatccaa caaccggaaa gaatgataac aaattacttc acgggtgtaa
     1441 atgaatttgt gacagacgag acaggacgca gagggaggag cgagggttca gggttcaggg
     1501 ttcagggttc agggttcagg gttcagggtt caggcgaagc gatgtcgcgg cgattattcc
     1561 gcggctggcg cggtgaccgc ccgggcggcc ggatggcgcg gatcggcttc cagcgtccgc
     1621 cgcaccgcct cggccgcctc ctcctcgcgg cccagttgat gcagcgcgac ggcgaggttc
     1681 caccagccgt ccgccttgtc cgggcggcgc cgggtcgcct cgcgcagccg ctccaccgcc
     1741 gacgccaggt ccccctcctg ccccgccagg accgccaggt tgatcagcgc ggacgtgtcc
     1801 tgcggattca gccggtccgc ctcctccagg agccgccgcg cttcgccgac ggccccgcga
     1861 tgcttcaggt cgagcgccac ccacgtatgc gcccgcgcgc tgcgcggctc ctgctcgagc
     1921 gttttccgcc atagcgcctc gttgctggac cagtccggca ggcgcgcgag gatcagcgcc
     1981 gcccccgccg ccgccagcgc ggccagcagc acggcccgcg tccgcggaga cggcgcccgc
     2041 gcgagcaggc ccgccgccag caggccggcg ccgggggcca gcaggtggag gtaccgctcg
     2101 gcgaagggat tgaagagcgg aaccacgttc gagacgggca ggaacgccag cagcatccag
     2161 ccgagggcca gcgcgagatc gggggcgcga cgccgcagcg cgaaggcggc cgcgatccag
     2221 cccgcccaaa ccgcccaccc ggccaggaac ggcagggcca gccaggaatc caccggatcc
     2281 acgacatgat ccaccagcag cggccacggc agcgcgagcc gccgcaaggc catgagccag
     2341 agcgcaggcg cggtccacag gttccgggga aaggccagcc ccaccccgtc cggtccgccg
     2401 cgcagggcct gcaccgatcc gctcgcccac cacaggacgg ccgcgcccag cgccagcgcg
     2461 gccgcggcgc cgcccatgcc ggccatccgc gcgcggccgg gccgcgcctc ggggaagcat
     2521 aaccagaccc agcccagcag cggcacgaag gccaccgccg attccttgga cagcagcgcc
     2581 agcgccagca gcggcacggc caaccatccc cacgcgtcgc ggcggcaggc cgccagcagg
     2641 aaggccaggc cgaacaggcc gaccagcagg tcctcgcgaa acgccggcga ctgcaccgct
     2701 tcgacgttca ccgggtgcaa cgcgaacagc agcgccgcgc cgaacggaat gaaacgcccg
     2761 cccgtgagac ggcgcaacag tccgtagaac aggagcgcgc agcccgcgtg cagcaggaga
     2821 ttcccggcat gatagccggc cggccgaagg ccccatagcg cccggtcgag caggtaggtc
     2881 gcgacgaccg ccggccgccg gccgtcgggg acggcgggat ccgtcagcgt ccggccggac
     2941 agcacgcgcc cccacgcgcc gggttcctgg aggaaggaat tttccgcgat cgaaccaagg
     3001 tcatcgtaca cgaacggata cgccaagccg cgcgtgtacg gcagaacggc cacgagcgcg
     3061 aggagcagga ctggcaccac gggatggttc agtctcagcg tcacacctca ccctttcagc
     3121 gcgcggaaga aggccgccca gaaggggtct tcgccgggat gctccatcgg cttcttcttc
     3181 ccgccggtca ccgcgtggat cccctccgcc gcgtcggtca tctccccgca gatggaggcc
     3241 aggataccct gatcctgcag ccggcgcagc acttcgttgg cggccttcgg gcgcacggcg
     3301 gcgatcagcg tgccctcgct gatgctgctg aaaggctcaa tatcgaacat ccggcatatt
     3361 tccgcgacgg cgggcgtcat gacgatctca tcgtagtgcg cgaccaggcc gagccccgcc
     3421 gccttcgcga attccagcag gccgcccgtg atgccgtact cggtggcgtc gtgcatggcg
     3481 gccacgccgc ggtcgcggac gccgaccgac gccgcgaggc gcgcgtcctc gacgacggac
     3541 atccggaaga acacgtcctc cgcctgcttc cggacggcgg ccccgaactc cttttcgatc
     3601 cggtccggga acatcgccgc gaagatgccc gtggcctcga cggcggggcc cttggtgatg
     3661 aggatccggt cgccggggcg gcagaacttc ggggagcagt aggcgtcccg cgggcccatc
     3721 gccatgaacg tcgccccgcc gaccatcggg taccggcagt tgtcgtaccg gccggtatgg
     3781 cccgtgacga tcgccgcgcc catcttctcg cactcgcggt ggatcacctc ccacatccgc
     3841 gcgaggtcgt cgtcggacag ttccggcggc aggttgagat cgatggagag gaacgcgggc
     3901 ggcaggccgg aggtgacggc gtcgctggcc aggatgtgga tcgcgaacca ggccgagcgc
     3961 tcgaacccgt actcgggcac gatgaacacc gggtcggcgg tcatcgccag ggcatactcg
     4021 ccgagttcca ggatgccgac gtcgatcccc tgctccgggc cgaccgcgac ctccggccga
     4081 cgggcgccga ggcggggctt gatgatcttc tggaaaaagt cgtccgtgat ttttccgagc
     4141 ggggccatgg cttttctcct tccgcgcttc gcgggagaat cggagcccgg gggcgggaat
     4201 gcaacggacc tcgacgggag gccccgttcg cgccgcttcc cttcgacggt attacccgta
     4261 caggttccag gggttctcct tcatggagga atctcagcct gccgagcaag ctcccccgcg
     4321 agcgatcagc cagaccatac cgcgttcgcg tcccgagtca agccggcgac cggccggcgg
     4381 ctactccgcg tacccgagcc gcttgagcat ccagtaattc cggctccagt tcgggtccac
     4441 cttgacccag agggacaggg tcaccgggcg cccgtagacg tcggccaact ccttttccgc
     4501 cgccgcctgc acccgcttga gcaggcgtcc cttcgcgccc agcacgatgc ccttctggga
     4561 attcttctgc acgaacaccg tgccctcggc gatccagcca cccgggcctt cctccaggct
     4621 ttcgatctcc accgccagcg cgtggggcag ttcctccttc agcacctgga aatacttctc
     4681 gcggactgtg tcggcgatga tcagcttgcg gggataatcg gtgacgacgt cgtccggaaa
     4741 cagcggcggg ccgggcggcg cgagccggaa cagggcctcg agcagcgcgc ccgtcccctg
     4801 ccccgtcagg ccggatacct cgacccagac cgggacagcc gggcccgtcc aggcggccct
     4861 gaacgcggcc gcgtgatcgg tgttccggtc gcattgattc agcgccgcca cgacgggcac
     4921 ggcttctttc cccaggcgcc tgatccagcc caggtcctcc tcgcggggcg gctgcgcggc
     4981 gtccacgacc aggaggacca tatccgcgcc ttcgaccgag gcgcgtgcgg ttcggttcat
     5041 gaccttgccc aagtcgtaag tggcccggtg aacacccggc gtatccagga agaccagttg
     5101 cccgcgcggc tcggtcagga tcgcgcgcac gagcgcccgc gtcgtctgcg ccaccgggct
     5161 gacgatgctg accttctcgc cgaccagcgc gttgaccagc gtcgacttgc ccgcgttggc
     5221 gcggccgacc acggccacga gggcggaagg cttgagttca ggcgtgccgg cgggaggcat
     5281 gggaaaacgc tagatttccg ccgtgtcgcc gggcttcggc agctcgacgc gcgccttggt
     5341 catctcccgg agcgccgccg ccagcgcctc gcgctggtcg ggctcgccgt gcaccaggaa
     5401 tatccgccgc ggccgggtct gccgcacgta ctcgaccagt tcgttccggt ccgcgtgcgc
     5461 gctgaacgcg tcgaggctgg ccacctcggc cttcggttcg aaccagtcgc cgaaaatccg
     5521 gaccttcttc tcgccgttca gcagccggcg gcccagggtg tacgacgcct ggtaccccac
     5581 catcaccacg gcgttcttcg ggttctcgat gccgtgcttg aggtggtgca ggatgcggcc
     5641 gtgctcgcac atgccggagg ccgcgatgat gatgcaggaa tccgggcagg cggtcaccgc
     5701 cttcgactcc tcgacgctgg cgatgtagcg gacccactcc gggtacatca cggagccgaa
     5761 gcgatggttc aggttccggt actcgtcgtc gaggtactcc gcgtatttct cgaaaagccc
     5821 gctgatggcg gtggccatcg ggctgtccac gtagacccgg atgttggaca gccggcccgc
     5881 ctggacgagc gaggccaggt ggaagatcac ctcctgcgtc cgctcgagcg cgaacgaggg
     5941 gatcatgacc ttgccgccgc gccgcagggt ccgctccacc acggcgtgca actggtcctc
     6001 cgccgcgccg gcctcgccgt gcaggcggtc gccgtacgtg ctttccagca cgagggcgtc
     6061 gaggccctcc atgggctcgg gatcacggat cagcggcagg tcgtcccggc cgaggtccac
     6121 ggcgaagccc gcgcgtttct ccgcgccatc cttgcgctcg gtgaagacgg tcagggcggc
     6181 gccgaggatg tgccccgcgt cgcggaaggt cgcctgcagg ccggccccca ggtcgacggg
     6241 ctccccgtag cggtatcccc ggaacagggc gatcgcggcc tcggcgtcgc ggagggtgta
     6301 gagcgggacg atcggctcct cgtccttccg gctggtcttc tggttcaggt aggcggcgtc
     6361 ctgttcctgg atatgggccg agtcccggag catcacgccg cagagcgccg ccgtggccgt
     6421 ggtggcgtgg atcggcccgc ggaagccggc gcgcgccagc gagggcaggt tgccgcagtg
     6481 gtcgatgtgg gcatgggaga gcacgacgga gtccaggcgc gaggggtcaa agggcaggcg
     6541 gctgttgatc tcgcgggcct ccttgcgatg gccctggtac agcccgcaat cgcgcaggac
     6601 gcggacgccg ccgcattcca cgatatgctg cgtgccggtc accgtatcga cgccgccgac
     6661 aaattgtatg cgcatcccgc ctctccggtt taacgcgtac cttaccgtcc gggcccggcg
     6721 aaggcaaccg tcccgaaacc cgtcggccgg ggatgccgcc tttaagaaag gtaacaatat
     6781 tattcacggc cgtaatgtat tttgttacag tggcgccgac tcccgcggtg cggtaggatg
     6841 aaagttcagg catgaaaaag gcggcaggca gaacgggcac gcggcggcgc gcgccggcga
     6901 agaaactgta ccgggcactg ttcaatgcct gggggcccca gcgctggtgg ccgggccgca
     6961 cgcgcttgga agtgatcgtc ggcgcggtgc tcacgcagaa cacggcgtgg ggcaatgtcg
     7021 agaaggccat cggccgcctc cgccgcgcgg acgcgctttc gctgccccgc cttcacgccc
     7081 tgcccctccg gcaactggaa ggcctcatcc ggccggccgg ctacttccgc gtcaaggcgc
     7141 gaaggttgcg cgccctgacg acgatgatcg tcgaccggca cggcgccctg cccgccctgt
     7201 tccggctttc cacgcccgac ctgcgcgacg aactgctaag ggtcaacggg gtcgggccgg
     7261 aaacggcgga cagcatcctg ctttacgcgg cgggccggcc ggccttcgtg gtcgatgcgt
     7321 acacccgccg gttcatgacg cggcacggct ggctcgctcc gaaagccacg tacgacgaga
     7381 ccgcccgcgc gctcgcgggc ggcctgccgc ggaacgtccg gctgtacaac gagtttcacg
     7441 ccctgatcgt ccggctgggc aaggactgct gccggccccg gccgctatgc ggagggtgcc
     7501 cgctggcgcc ctggctgccg cggggaggac cgcggccatg agactggaac cggccctcgg
     7561 actggccctg gccctgttca cgctgggctg cacgtccccc gtaacgacgg cgcccgcccc
     7621 cgcgccggcg gccccgggct ttgtgggccg cgtggtccgg gtcaacgcgg cgcagcggtt
     7681 cgtgatcgtg caatgtgagc gcttgccttc cgagggggac acgctcacac tgtatcgggg
     7741 taaagagccg gcgggccggc ttcgggccac gggacccttc cgccccccgt acgtggcggc
     7801 ggacgtcctc gagggcgccc ccgaacgcgg ggatgcggtc cgcgcggagg cccggataac
     7861 ggcggaaccg gagacaagcg ggagcaatct atgaacctcg catttctgaa ccgattgagc
     7921 gcgtgggaaa tcatcctgat cctcgcggtg gtcctcctgc ttttcggggc caagaagctg
     7981 cccgagctgg cgcgttccct cgggcgcagc cttcaggaat tcaaaaaagg ccagcaggaa
     8041 ggcgcatccg acaagccgga tgacgaaggc aagccgggcg gaccgcccaa ggcttgatcc
     8101 cggaccgccg cgagctcact cccccgtcgc gccgtccggc gtgagggcgg gcggttccgc
     8161 ggagtcccag ggtaactccc gcagaagttc ccactcgggc cgccccatcc agtcggccat
     8221 cgcgtcggtt ccgacgtgac ccgcggcccg ccgcagggtc tcgcatgcct cgtcggtccg
     8281 gttctggcgc acgtagcaca cggcccgggt gaaaagcgcc atggcattgg cgggatcttc
     8341 gttcaacacg ccctccagcg tcgccagggc cttgtcatgc tcgccggcgc ggctcaaggc
     8401 gtcggccagc tcgctctgga catcaaggtt gtcgaactcg atgttgacga ttttccgcag
     8461 gtgcgggatg gccgcgcggg gctggtcggt tttcaggtag gaggtggccg ccagccggtg
     8521 ggcctccatc gaatacggat ccgtctcgac catccactcg gccacctggc ggcacaggtc
     8581 atactcgccc agctttccca gcgcggcgcc gagcaggaga cgggcctcgg gatcggccgg
     8641 ctgggcatcg agcgcttcgc gcaactgaac cacggcctcc gccgggcggc ccgcatcggc
     8701 cagcacccgc gccagcgtca ggcgcacggc gacggccgag ggataatcct tcaacgcctg
     8761 ttccaagcgt tgttccgcct cgcccgccag gctcttctgc cggaggcctt ccacctcggc
     8821 caggcgatcc ggcatctgcc gccacgcggc gatgcgcgcg cccaggtcgg atgggatcgc
     8881 gttggaggag gccggcggcg gcgtggccgg cggggcatcg gcctcgcggg cctcccgctg
     8941 aatctggatg cggtatttca tgaaattcgt cagcaccacg agcagcatga ggccgccaac
     9001 caccaggctg aaggcgatca gggcgctgtt caggcgggaa gacagaaaat agcgccagtg
     9061 gaaccggcgg ctgccgcttt ttcggcgacg gcgtccgtag tgcgaagaag gcgcgggacc
     9121 ggccgggggc gcctcccccg gggtggccgg gtcggcgccg gccgccggcg gcgattggga
     9181 ggccgaaggc atccggccat gacgcaacag gaaccgggac cgacgggaca cgtgatacgg
     9241 ccggtccgct ttctggtaga tatcgcccgt cggctccatg tcggctcact ctacgcactt
     9301 ccaatgcgga ttcaaggcgg gaagtcggcc ggtcagcgat cggccggcgc cagcggccga
     9361 agctcctcgg gaagggccag ggacgggtcc atggacagca ggtcgcgcag cacctcggac
     9421 gccaggtgct cctgcccgcc gcggaaatgc gccagggcca ggcgcaggcc gatctccatc
     9481 tgcgtatggg tcggatacca gcgggaccgg ccccaggccc gctccagcac gcgcgccgcc
     9541 tcgtcgtact gcttatgctc catcagggcg taaccgagcg tgtccatgaa caacgggttg
     9601 gcgggatcga gcacgagggc ctggcgcgcc aggcgcgcgg cctcgtcgag gtcgcggttc
     9661 tgctcggcca gcaggtaggc caggttgttg taggcccgcg cctgccgggg atcctcccgg
     9721 atggcgcggc gatagagctc ctccgcctcg ccggcgcgat ccagcccgcg gtacaggttg
     9781 ccgagggaaa ccagcatggc cggcgaacgg gcgccggcct ccagggcggc ctcgtagtcc
     9841 cggaccgccg gggccgcctg gccggccgcc tcgaagtacc ggccgcggaa catgcgctcc
     9901 tcggcgccca gttccaggcg cgcgtcgtcc ggcggacgga tgagcagcgc gcggtggtcc
     9961 gcggcgcgcc agcggcgcag gaaatcggcg gacggctggc cggcgcggcc gccgggcacg
    10021 cggtccacca gccggaggct cttcgtgtcg tcgaagccga cgaccagcgc gtcgacgagc
    10081 gacgccccgt ccgcggcctc ctccagccgc acgagcacgg ggagtccggc gcggagccat
    10141 tccttcaggc gctcgtccga cgcggagcag ggataggccc agagcccttg gcgccatacg
    10201 gtgcgcacga cggcttcctc ggaatccggc agctcggccg ggtccgcgcc gggttccgcc
    10261 agcgaaccgg gccgcacggg ccttccccag aagcggagca cggccgcgag gggcgcctgc
    10321 tcgccgcagg acttgaagtc ggaaaccggc ggaaccttcg ggacccaggc ggcgcgctcg
    10381 ggctcgcggc gcgcggccag tcggaaatcg cgatccggcc cgggcccggc gcaaccggcc
    10441 aggacgcaca gcgccgggat cagcaggagg aggcgccggt cctttcgcag gggcccgcac
    10501 tggccgcgcg cgccggacat taccacgggg ccagtttatc caggacgttc tgcacgccgg
    10561 gcgtggcgcg cgcaatgccg atggcccggg accgcacgta gctgctggag atggcccctt
    10621 ccatgatcac caccccgtcg cgcacgaaaa cgccgaacga aaacggcgcc gtcacgggat
    10681 cctcttcgag gcgacgctcc acttcctgcg tcagccgctc gtccgccagg gctttgtcat
    10741 ccgccgggcc gggagtcgtc gcgcagccgc cggcgcccag gaccaccgcc atacccagca
    10801 ggatgagcat tttcgacgct ttcatagcac cttcctggcc cgtcgccgcg agccgttttc
    10861 cggggagtag agtatgccag ccgcatcctc ccgtcaatcg gcggcttccc atccccgcgc
    10921 ggaagctgat acagtgctgg cgaccctcgc cgaacggacc ggcgggacag gagcgcaacg
    10981 tgaaaaaaac cgttattctc attcttctcg cgggatggac gctggcggcg ggcgcggcgg
    11041 atcccgtgat ccccgacctc tacgagcggc aggtgctgcc gaacctggac agctcctgga
    11101 tcagcggcat cacctactac ctcgaaaagg gaggcgacag cgggatgctg gtcgtcgagc
    11161 tcaagggcca gaagatcacg tacctggacg tccccctggc cctctggcag gagttccagc
    11221 agacggattc gccgggctcg ctgtacggca agaaggtgaa gaacaagttc gagcgcgtcg
    11281 agggcgatcc gctctggatg cggtacgacg ccgcgccgcc cgagacgctc acgggccagg
    11341 tgacctgttc cttcaacgag gaatgcgaga cggtcgtcgt ccaggcgctc ggcgaggccc
    11401 ggcgcacggt ccgcgtggcc gcctacgcct tcacccgcag ccgcatcgcc cgcgccatgg
    11461 tcgaggcgcg cgagcgcggc gtggatatcc agatcaagat ggactcgcag caggccgagt
    11521 atccgctggc ggtgcgcacc atcgagtggc tgcggaacaa gggcatcccc gtcacgctga
    11581 tcaccatgtc cggcgactac gcggcgatgc acaacaagtt catcgtcatc gacgaacagg
    11641 tggtgttgac ggggagcttc aacttcacca cgaccgccgt cacggcgaac tgggaaaaca
    11701 tgctgcgggt cgactcgccg gagatcgcgg cgcggttcct cgcctactgg gaagtgatcg
    11761 cctcgaattg atcacggggc ggcgggctcg aacagctccc ggaactcgag ctcccgggcc
    11821 cgctggatga cttgccggaa ccgctcctcg tcccggc
//
LOCUS       DAOOTK010000106        11530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3710_length_11530_cov_4.688802, whole genome shotgun sequence.
ACCESSION   DAOOTK010000106 DAOOTK010000000
VERSION     DAOOTK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_11530_cov_4.688802"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1229
                     /locus_tag="PK634_11055"
     CDS             117..1229
                     /locus_tag="PK634_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOW98314.1"
                     /translation="MQEFHATCIPGAEEVLCDELRELGFASVRLNRGGIPLRGEWRDG
                     WRACLQSRIAQRILVLLRRFPAADESALYAGAHDTDWNRLLSPRTTFAVSAVCRGSAL
                     QNANFAALKVKDAICDRLRDETGARPAVSRDDPDVRVQLYLANDKAALYADLSGESLH
                     RRGYRLETGEAPLRETLAAAILRLSGWDRATPLVDPMCGSGTIAIEAAQWASNMAPGL
                     GCERFGFERWASFGESEAAALRELRGRLREEVRPSRPRIIAGDADAAVLDVAKANARR
                     AGVRLSFRHSSIQDLQSGGEKGVIVTNPPYGVRLAAEPDLVRGVAAALTRMHGWRACL
                     LAGSPEYERALPLRPVMKMSLPNGDIDCDFLAYDIP"
     gene            1602..2051
                     /locus_tag="PK634_11060"
     CDS             1602..2051
                     /locus_tag="PK634_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOW98315.1"
                     /translation="MKRHTINPVAWMLGLALLAAGCEDEVPEAMHICRTEWILKSLGP
                     VGAETPIIANSRVELRFACDDTFSGNSGCNSYSGQFSATDAGDITIGQTAMTEMACLD
                     AALMDQEARYIGALTRVTRFEREDDTLRLYYNGGAEDLYFEVCTDCD"
     gene            complement(2138..2590)
                     /locus_tag="PK634_11065"
     CDS             complement(2138..2590)
                     /locus_tag="PK634_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98316.1"
                     /translation="MKKLATLTLIASIGLFAGSNKADAEQIWFKGFGARYDYVKYGTW
                     LATQIRGDIGVWPVASGHSAGAVYTDNGWKNVYWTDAKWQANVQNPYGGLDEAWNVWL
                     SASGPNGRYMGMPFTPFTIETAVYVKNASGQWFWDNNVGYNYRYHVLR"
     gene            3176..4849
                     /locus_tag="PK634_11070"
     CDS             3176..4849
                     /locus_tag="PK634_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW98317.1"
                     /translation="MALSAACFVVKHSPVWILPIIVADVIDVVALPARHDLRRLWVDG
                     AIMLALLVQNIPFELVHVTQLSKVIRRIEKELRGALTQRLQQLSIAFHDRSHTGAMQS
                     KMLRDVEAVEGLCRFCFVMLLNGFTSIAFALGVTFYNRAPAMALFYFITIPVSVALIR
                     FFHRRIHDMNKGFRTRIEAMSARVAEMIAMIPITRAHGAEQHEISTIETHLTAVRESG
                     LRLDVLNALFGAVAWVSFQVPVLLSLMVAGYLAFHGRITPGEVTMYSSYFMMILGSVN
                     MILNSYPMIARGLESVRSIREVLDSPDIERNEGKVAVDDVRGAFAFDGVSFAYPSGEF
                     HALEDIRLEVRAGETLAVVGESGAGKSTLMALVIGFHRPTVGRILLDGRNMEELDLRS
                     FRRFLAVVPQQTILFSGSIRDNITYGLPDVPEEQVQRVIDAANLRELVNRLSAGLDTL
                     VGERGARLSGGERQRVSIARAMIRQPRVIVLDEATSSLDAASESLVQEALQRLIRDRT
                     TFIVAHRLSTIRHAHRIAVLKRGRLVELGTPDELMARDGEFARLRRFQA"
     gene            4922..6286
                     /locus_tag="PK634_11075"
     CDS             4922..6286
                     /locus_tag="PK634_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOW98318.1"
                     /translation="MAFLKRIDPDQITGGRILPAILGLSMPMLVGALLQNAQSLIDLF
                     WVGRLGSVAVASVAMAGTILMLLYPMLMGLSTGTLALVARAVGAGRTDDAGSAAGQSL
                     FLSVILGGLSAAVGWLVAEPLLHVMGADADVTACGATYLRICLVGSFTVYLLFIGNAA
                     LQGAGDAHTPMYVMAASNALNIVLDPLLIFGLGPFPRLGVAGAAWATVLAQAVAAALA
                     LWVLLGGNARLHAHWRYWKPDLPLAWRILKIGIPGSGQMLSRSLMNVILMWIVATCGT
                     TAVAAYGIGMRFHFIILMPAFALGGAAATMMGQNLGAGKPDRARRSVWVASGLDALFM
                     LLAGAALMLFARPLIGAFNGQEEVIATGARYLHVVSPFYVFAGFGIVLGRGLNGAGDT
                     LGPMIITVLALWGLQVPLALYLSRVLAPATEGIWWAIAAAFVAQGIMMVAWFESRRWQ
                     WKRI"
     gene            complement(6307..6380)
                     /locus_tag="PK634_11080"
     tRNA            complement(6307..6380)
                     /locus_tag="PK634_11080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6344..6346),aa:Pro,seq:ggg)
     gene            6541..7509
                     /locus_tag="PK634_11085"
     CDS             6541..7509
                     /locus_tag="PK634_11085"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOW98319.1"
                     /translation="MKHPATHECTGTGAEYCLCRAATGLLSLYSGGPEDRADSLLGSY
                     PNTTNIEAGLRILIDVMLPGRMSQAEIQAEELGLFLVRRLGEAWRLLRPEVERAVPFR
                     WIGQAARMEGAPKPVDPRGESVRIMQAFVGRLGDIRKLLVEDIRAAYEGDPAALTYAE
                     VQLAYPGLLAIASHRLAHEFYKLKVPIVPRVMSEWTHSQTGVDIHPGAEIGHGFFIDH
                     ATGVVIGETTHIGHRVKLYQGVTLGARSFSLDAQGQPVKHVKRHPTVEDDVVIYAHAT
                     ILGGDTVIGRGSTIGANVFLMESVPPKSFVTSKHAELHIKRGNEET"
     gene            7603..7941
                     /locus_tag="PK634_11090"
     CDS             7603..7941
                     /locus_tag="PK634_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98320.1"
                     /translation="MNRILAILLGTQLLYSISDFMGRAYMSRHGFRPAAFWSLWFLVY
                     LVIRQVAMVGQLYLFVHVPLGKSMALLGAVSIVISNALGWLFLGEVLSLYGYLGVFLA
                     VTAILIMAAR"
     gene            complement(7952..9550)
                     /locus_tag="PK634_11095"
     CDS             complement(7952..9550)
                     /locus_tag="PK634_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOW98321.1"
                     /translation="MNQALLPVILEGLVLLAIYAAAVGLTRLFCRSRSEEAIGSRAFV
                     DLPARPLLILVMTYGVLALLRLHGGAASWLDAHPKHASAWLTFWVVVLALGLIEAVVR
                     NLGRVGGQPIRIPQLMRNIIRGLTLLLAAFVILKYQLGINISPLLASTALVTAVVGFA
                     LQGVLGNLLGGMSLHVVGSVLPGDWLAIGDTEGEVIETNWRETRLRTIAGHILVIPNG
                     TVASSVIHNMTRPTPLRRHTINVGASYSDAPGEVIEALLESARAVPDVLREPAPSAVL
                     VEYKDFGINYRLRFWTNRYYDRTVLEGDVMRMIWYRFKRRGIEIPFPMSDKLLNDFMA
                     VVYRQRRMPPESEETRDTARDLLRSDFVRKLLVDEKGQALLSEADLAGLAPAIRRVQF
                     TAGETIFLQGEAGESCYVLVRGTVRGRVEYSDLRQAHEFDLGPGSLFGEMSLVTGLPR
                     TATLVSPGETEMLMISREAFTRLLALRKEIPEALARIVARRAEANLAALEQLKSLGPA
                     NVAETLKKENILRRFLRLLGVGAG"
     gene            complement(9547..10368)
                     /locus_tag="PK634_11100"
     CDS             complement(9547..10368)
                     /locus_tag="PK634_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOW98322.1"
                     /translation="MKITLGGVRGTSSMARSEFMRYGGETTSVLVQGRAGETVILDAG
                     TGIRALGESWGAEPPREVLLLLTHYHLDHIVGWPSFSLLYRKGVAIRVASSLTDGPAV
                     ESILSQLMKQPFWPVQMDSVQAAVTFEKTPATFGRLRISSCPVHHPGGCVAYRLDEPA
                     TGASVVFATDIEWSVSSPEEQERFLAFCRQPAPCRLLLFDGHYSRATYPAFKGWGHST
                     WEEAVEVARKAGAGQLLVIHHAPDSRDEYLERLDRRLLAAYPGSSLARDGMEVEL"
     gene            complement(10373..>11530)
                     /locus_tag="PK634_11105"
     CDS             complement(10373..>11530)
                     /locus_tag="PK634_11105"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOW98323.1"
                     /translation="IHTTMLDNLLAAQFVRDARPWMVWLATLLLALLSGVAVTLGRKA
                     WQNVAAFVVFLPIPPLAGLAAYRAGVWWPVAVGEVAVLLAMVGAVVVNYATEGRQKAF
                     IKSAFKFYLGETVIDQLLADPSRLSLGGEKRELTIFFSDIEKFSSFSERLDPTQLIGL
                     LNEYLSEMGEIIKEEGGYLDKFIGDAIVAFWNAPVAQPDHAARACRAALRCQRRLAER
                     RADWQEKYGAVVKMRIGINTGDVTVGNMGSRDRFNYTVLGDAANLASRLEGANKAFGT
                     YFMVSESTWTGTGGAFAGRQLGRLTVVGRKQPVAVYEPAGLPGETAPAWHADFERALK
                     LFFEGRFGDALAVFEQLTDDPASRAYAERCRLYRANPPADWAGVWNLLEKG"
BASE COUNT         1916 a         3873 c         3711 g         2030 t
ORIGIN      
        1 cctgcccgaa gactgggatt acctggagga cttcctgcgc cgccgcgcca cgcgcggccg
       61 gggcggcgaa atggcgcttt tcgtgcacaa gctccggatc aagagcggcg tgtaacatgc
      121 aggagttcca cgccacctgc atccccggcg ccgaggaggt gctgtgcgac gaattgcgcg
      181 agttgggctt cgccagcgtg cggctcaatc gcggcgggat ccccttacgc ggagagtggc
      241 gcgacggctg gcgggcctgc ctccagagcc ggatcgccca gcggatcctc gtccttctcc
      301 gccgcttccc cgccgcggac gaatccgcgc tgtacgccgg cgcgcacgac acggactgga
      361 accggctgct gtcgccgcgg acgacgtttg ccgtgtccgc agtctgccgg ggcagcgccc
      421 tgcaaaacgc caatttcgcc gccctcaagg tcaaggacgc gatctgcgac cggctccggg
      481 acgaaaccgg cgcccgcccc gcggtgtcgc gggacgaccc cgacgtgcgc gtgcagttgt
      541 acctggcgaa cgacaaggcc gcgctgtacg ccgacctgtc cggcgaatcc ctgcaccgcc
      601 gcggctaccg cctggagacc ggcgaggcgc cgctccgcga gactctcgcc gcggcgatcc
      661 tccgcctgtc cggctgggac cgggcaacgc ccctggtgga cccgatgtgc ggctccggta
      721 ccatcgccat cgaggcggcc cagtgggcgt ccaacatggc gccgggcctg ggctgcgaac
      781 gcttcgggtt cgagcgctgg gccagcttcg gcgagtccga ggccgccgct ctgcgcgaac
      841 tgcgcggccg gctccgcgag gaggtccgtc catcccgccc gcgcatcatc gccggcgacg
      901 ccgacgccgc ggtcctggac gtcgcgaaag ccaacgcccg ccgcgccggc gtgcgcttgt
      961 cgttccggca cagctccatt caggacttgc aatccggcgg ggagaaaggc gtgatcgtga
     1021 ccaatccgcc ctacggcgtg cgactggcgg ccgagccgga cctggtccgc ggtgtcgcgg
     1081 cggcgctcac ccggatgcac ggctggcggg cctgcctgct ggccggctcc ccggagtacg
     1141 agcgcgccct tcccctgcgg ccggtcatga aaatgtcgct gcccaatggc gacatcgact
     1201 gcgatttcct tgcgtacgat atcccgtgag cagcgcgcga ccaccccgct ccaggagggg
     1261 ccgccgcctc ccgtacaggt gaatccgcgc gccaccgcgc cgaggccatt cagggtgatg
     1321 caggtttccg tattccggcc gttcactacc gcgtgttccg ctccacccac gctcgccagc
     1381 gtaacgccga gtccaaccgc ggcggcccga ctgcccgcca gccagcggcc tgacatgctt
     1441 tcattaggcc tcctcggcaa gtgtcgctta cagttccggt aattccgacg cacgcatatg
     1501 ggcacagcac gggctgaccc ttccgaatcg gcctgcgaca caatgatcct tggcttcgtc
     1561 ccgtctgcaa gtgtaccttt cccgcaataa aaggatcgcg catgaaacgg cacacgatta
     1621 atccggtggc gtggatgctt ggcctggctc tcctggcagc aggctgcgaa gacgaagtgc
     1681 ccgaggccat gcatatctgc cgcaccgaat ggatactcaa aagcctgggc ccggtcggcg
     1741 ccgagacccc gatcatcgcc aatagccgcg ttgaactgcg gtttgcctgt gacgacacgt
     1801 tttccggtaa ctccggctgc aattcctaca gcgggcagtt ctcggccacc gatgcgggcg
     1861 acatcacgat cgggcagacc gccatgacgg aaatggcctg tctggatgcg gcgctgatgg
     1921 accaggaagc ccggtacatc ggggctctga cccgggtgac gcgcttcgaa cgggaagacg
     1981 atacgctgcg gctgtactac aacggcggcg ccgaggacct gtatttcgag gtatgcacgg
     2041 actgcgactg acggcctgtc ttgggcacga aaggaaaagc ggcccccggg aatccccggg
     2101 agccgctttt ttgtgttttt attaaaccgc cccaagccta ccgaaggaca tggtaacggt
     2161 agttgtatcc gacgttatta tcccagaacc actgcccgga tgcgtttttt acgtataccg
     2221 ctgtttcgat ggtaaacggc gtgaacggca tccccatgta gcggccgttc ggaccgctgg
     2281 cggacagcca gacgttccat gcctcgtcca gccccccgta agggttctga acattggcct
     2341 gccacttggc atcggtccag tacacgttct tccaaccatt atcggtgtaa accgctccgg
     2401 ccgaatgacc tgaggccacg ggccagacac cgatgtcacc gcgaatctgg gtcgccagcc
     2461 aggtcccgta cttgacgtag tcataacggg cgccgaagcc cttgaaccag atctgctctg
     2521 catctgcctt gttgcttccc gcaaacaagc caatagacgc aatcagcgtt aatgtcgcca
     2581 gttttttcat tttgtctcct ttccattgac ttttcagtaa aacatccttt cgctagccgt
     2641 ctcataacca gcagactagt agaacgtttt actgcaacca cactacaaga gaccaagtgg
     2701 catgtcaagt tagccagtga ctatattgcg attagtatct tgttattatt aaacagattg
     2761 taatcacctg acacttttgc cagaatttta atttgagtaa tgagtttaga tgagatggtt
     2821 tgagccatta aagataatca taattacata tatatcaata tattacgata tatgacaaga
     2881 tcagtatgaa attcgtaatg tgattcacga ttgtcaatat atatatcata ttatacgtaa
     2941 tattaatata taatgacttt ggattattaa tcgtatcaga aaatatttat tgacattcgt
     3001 tggatatttg acttaaggtg atttcttgat gataggccac cacggaccgg ttgcagcttg
     3061 ccatcctcgc cggtttccgg tcgaatcact gccaatgcag ccttcggaac ccagccagcg
     3121 gagagagtgt gcctttggaa gcttgtgggc ccttttccgg cctgatgcat ggcgtttggc
     3181 cctgtcggcc gcttgtttcg tcgtcaagca ttccccggta tggattctcc cgatcatcgt
     3241 ggcggatgtc atcgacgtgg tcgccctccc ggccaggcat gatctgcggc gcttgtgggt
     3301 ggacggtgcg atcatgctgg cgctgctggt ccagaacatc ccctttgaac tggtccacgt
     3361 cacccagttg agcaaggtga tccgccgcat cgaaaaggaa ctgcgaggcg ccctgaccca
     3421 gcggctgcaa cagctctcca tcgcgttcca tgaccgctcc cacacgggag ccatgcagtc
     3481 caagatgctg cgggacgtcg aggcagtgga aggcctgtgc cgattctgct tcgtcatgct
     3541 gctcaacgga ttcaccagca tcgccttcgc cctgggcgtt actttttata accgcgcccc
     3601 ggccatggcc ctgttctatt tcatcacgat cccggtctcc gtagccctga tccgcttctt
     3661 tcaccggcgt atccacgaca tgaacaaggg attccggacg cgcatcgaag ccatgtccgc
     3721 gcgcgtggcg gagatgatcg ccatgattcc catcacccgc gcccacggcg ccgagcagca
     3781 cgagatttcc accatcgaga cccacttgac cgccgttcgc gaaagcggcc tgcgcctgga
     3841 cgtcctcaac gcgctgttcg gcgccgtggc ctgggtttcg ttccaagtgc ccgtgctctt
     3901 gagcctgatg gtcgccgggt acctggcgtt ccacggccgg atcacgccgg gcgaggtcac
     3961 gatgtattcg tcgtatttca tgatgatcct cggctcggtc aacatgatcc tgaacagcta
     4021 tcccatgatc gcccgcggcc tggagtcagt ccgctccatc cgggaggtgc tcgattcgcc
     4081 cgacatcgaa cgcaacgagg gcaaggttgc cgtggacgat gtccggggcg cctttgcgtt
     4141 cgacggcgtg agtttcgcct acccgagcgg ggaattccat gcattggaag atatccggct
     4201 ggaggtgcgg gccggggaaa cgctcgcggt cgtgggcgaa tccggcgcgg gcaaaagcac
     4261 cctcatggcc ctcgtgatcg gattccatcg ccccacggtc ggccgcatcc tgctggacgg
     4321 caggaatatg gaagagctgg acctgcgaag tttccgacgg ttcctggccg tggtccccca
     4381 gcagacgatc ctcttctcgg gttcgatccg cgataacata acgtacggtt tgcccgatgt
     4441 ccccgaggaa caggttcagc gcgtgatcga cgcggcgaac ctgcgggaac tcgtgaaccg
     4501 tctctcggcc ggcctcgaca cgcttgtcgg cgaacgcggc gcgcggctct cgggcggcga
     4561 gcgccagcgg gtctcgatcg cgcgggccat gattcgccaa ccccgggtca tcgtgctcga
     4621 cgaggccacc tcctccctgg acgcggcctc cgagagcctg gtccaggagg cgctccagcg
     4681 cctgatccgt gaccggacga ccttcatcgt ggcgcaccgg ctttcgacga tccgccacgc
     4741 ccaccggatc gccgtgctga aacgcggccg gttggtcgaa ctcggcacac ccgacgaact
     4801 gatggcccgc gacggcgagt tcgcccgtct ccgccggttc caggcgtgat cggcggttcc
     4861 ctttgacatt acgtcccgct cgtgcagtat ggtaccccca tttaacgagg cggcctggcg
     4921 catggctttc ttgaaacgaa ttgatccgga ccagatcacg gggggccgca tcctgccggc
     4981 catcctgggc ctgtccatgc ccatgctggt gggcgccctg ctccagaacg cgcagagtct
     5041 catcgacctc ttctgggtcg gccggctcgg ttccgtggcc gtcgcctcgg tggccatggc
     5101 cggcactatc ctgatgctgc tctacccgat gctgatgggc ctctccaccg gtacgctggc
     5161 cctggtggcg cgcgccgtcg gggcaggccg gacggacgac gcgggctccg ccgccggcca
     5221 gtcgctgttc ctgtcggtga tcctcggcgg cctttcggcg gccgtcggct ggctggtcgc
     5281 ggagccgctg ctgcacgtca tgggggcgga cgcggacgtc acggcctgcg gcgcgaccta
     5341 cctgcgcatc tgcctggtcg ggagcttcac ggtgtacctg ctcttcatcg gcaacgcggc
     5401 gttgcagggc gcgggggacg cgcacacgcc gatgtacgtg atggcggcgt cgaacgcgct
     5461 gaacatcgtt ctcgacccgc ttctcatctt cgggctcggc cccttccccc ggctgggcgt
     5521 ggccggggcg gcgtgggcca cggtgctggc gcaggccgtc gcggccgccc tggcgctctg
     5581 ggtgctgctg gggggcaacg cgcggctgca cgcccactgg cgctactgga aacccgacct
     5641 cccgctggca tggcgcattc tgaagatcgg cattccaggc tcgggccaga tgctctcccg
     5701 gagcctgatg aacgtgatcc tgatgtggat cgtggccacc tgcggaacga cggccgtggc
     5761 cgcgtatggc atcggcatgc gctttcactt tatcatcctg atgccggcct tcgcgctggg
     5821 cggggcggcg gcgacgatga tgggacagaa cctcggcgcg gggaaaccgg accgggcccg
     5881 gcgttcggtc tgggtcgcct cggggctcga cgcgctgttc atgctgctgg cgggggcggc
     5941 gctgatgctg ttcgcgaggc cgctcatcgg cgcgttcaac ggccaggaag aagtcatcgc
     6001 caccggcgcg cgctacctgc acgtggtgtc gccgttctac gtcttcgcgg gattcggcat
     6061 cgtgctcggc cgcggcttga acggcgcggg cgacacgctc ggccccatga tcatcacggt
     6121 cctcgccttg tggggcctgc aggtgccgct ggccctgtat ctctcccgcg tcctggcgcc
     6181 ggccacggag ggcatctggt gggccatcgc cgcggccttc gtcgcccagg ggatcatgat
     6241 ggtcgcgtgg ttcgagtccc ggcgctggca atggaaacgc atataggccg gaatccggcc
     6301 ccggtgtcgg ggcgacgaga tttgaactcg tgaccttttg caccccaagc aaacgcgcta
     6361 ccaggctgcg ctacgccccg acaccggggt catgaaacgg cgcggctgtc caagcaagcc
     6421 gagacgctag cacgcggccc ggggccggtc aaaccgcttt tccgtccccg gcggaaccgt
     6481 tcttgattcg cgggaggggg tcgggtaggt tgcccgcgac gagcaaaggg agaaacgggc
     6541 atgaagcatc cagcgacgca cgaatgcacg gggacggggg cggaatactg cctgtgccgg
     6601 gcggccaccg gcctgctctc gctgtactcg ggcggccccg aggaccgcgc ggacagcctg
     6661 ctgggctcct atcccaacac gacgaacatc gaggcgggcc tgcgcatcct catcgacgtc
     6721 atgctccccg ggcgcatgtc ccaggcggaa atccaggcgg aggaactggg cctgttcctg
     6781 gtccggcgcc tgggcgaggc gtggcggctg ctgcggcccg aggtcgaacg ggccgtcccc
     6841 ttccgctgga tcggccaggc cgcccgcatg gagggcgcgc cgaaacccgt ggacccgcgc
     6901 ggggaatcgg tccgcatcat gcaggccttc gtggggaggc tgggcgatat ccgcaagttg
     6961 ctggtcgagg acatccgggc cgcgtacgag ggtgacccgg cggcgctgac ctatgccgag
     7021 gtccagttgg cctaccccgg cctcctggcg atcgcctcac accggctggc gcacgagttc
     7081 tacaagctca aggttcccat cgtccctcgc gtgatgagcg aatggactca ctcgcagacc
     7141 ggcgtggaca tccaccccgg cgcggagatc ggccacgggt tcttcatcga ccacgccacg
     7201 ggcgtggtta tcggtgaaac gacgcacatc ggccaccgcg tgaagctgta ccagggcgtc
     7261 acgctggggg cgcgcagctt ctcgctcgac gcgcagggcc agccggtcaa gcacgtcaag
     7321 cggcacccga cggtggagga cgacgtggtg atctacgcgc acgcgacgat cctcggcggc
     7381 gacacggtca tcggccgggg ctcgacgatc ggggccaacg tgttcctgat ggagagcgtc
     7441 ccgcccaaga gcttcgtgac cagcaagcac gccgagttgc acatcaagcg gggtaacgag
     7501 gaaacctgac gccgcggccc gcgtcggtaa caatactctt cacacccgtg aagtgatttg
     7561 ttacccccgg agcggaccgt cgaaggcggg ttgcgcgcaa ccgtgaaccg aatcctggcc
     7621 attctgctcg gcacccagct gctctactcc atcagcgatt tcatggggcg cgcgtacatg
     7681 tcgcggcacg gcttccggcc cgcggccttt tggagcctct ggttcctggt ctacctggtc
     7741 atccggcagg tggccatggt cggccagctc tacctcttcg ttcacgtgcc gctcggcaag
     7801 agcatggcgc tgctcggggc ggtgtccatc gtgatcagca acgcgctggg ctggctgttt
     7861 ctcggcgagg tgctttccct ctacgggtat ctcggggtgt tcctcgccgt gacggccatc
     7921 ctgatcatgg cggcgcggta gcggcggccg gtcacccggc gcccacgccg agcaggcgga
     7981 ggaaccggcg caggatgttc tccttcttca gggtctccgc cacgttcgcg ggacccagcg
     8041 acttcaactg ctccagcgcc gcgaggttgg cctcggcgcg gcgcgcgacg atgcgcgcca
     8101 gcgcctccgg gatttccttg cgcagggcca gcagccgcgt gaaggcctcg cgggaaatca
     8161 tcagcatctc ggtctcgccg ggcgagacca gcgtcgccgt gcgcggcagc cccgtgacga
     8221 ggctcatttc gccgaacagc gagcccgggc ccaggtcgaa ctcgtgggcc tgccgcaggt
     8281 cgctgtattc cacgcggccg cggacggtcc cgcgcacgag cacgtagcac gattcgcccg
     8341 cctcgccctg caggaagatc gtctccccgg ccgtgaactg cacgcggcgg atggcgggag
     8401 ccagcccggc caggtccgcc tccgacaaca gcgcttggcc cttctcgtcc accagcagct
     8461 tgcggacgaa atcgctgcgg agcaggtccc gggccgtgtc gcgcgtctcc tcgctttccg
     8521 gcggcatccg gcgctggcgg tagacgacgg ccatgaagtc gttgaggagc ttgtcgctca
     8581 tcgggaacgg gatctcgatg ccgcggcgct tgaaccggta ccagatcatc cgcatcacgt
     8641 cgccctcgag cacggtgcgg tcatagtacc ggttcgtcca gaaacggagc cggtagttga
     8701 tgccaaaatc cttgtattcc accagcacgg ccgagggcgc cggctcgcgc aggacgtccg
     8761 gcaccgcccg ggcggattcc aggagggcct cgatcacctc gccgggcgcg tccgagtagc
     8821 ttgcgcccac gttgatggtg tgccgccgca acggcgtggg ccgcgtcatg ttgtggatga
     8881 cagaggacgc caccgtgccg ttggggatga cgaggatgtg gcccgcgatg gtccggagcc
     8941 gggtctcgcg ccagttggtc tcgatgacct ccccctccgt gtcgccgatg gccagccagt
     9001 cgccgggcag gacggacccg accacgtgca gcgacatgcc gccgagcagg ttgcccagca
     9061 cgccctgcag ggcgaacccc acgacagccg tgacgagggc ggtggacgcg aggagcggcg
     9121 agatgttgat gcccaactgg tacttgagga tcacgaaggc cgccagcagc agggtcaggc
     9181 cccggatgat gttgcgcatg agttggggga tgcggatcgg ctgaccgccc acgcggccca
     9241 ggttccgaac gaccgcctcg atcagcccca gcgccagcac caccacccag aacgtcagcc
     9301 acgcggaggc gtgcttggga tgcgcgtcca gccaggaggc cgccccgccg tggagccgca
     9361 gcaaggccag cacgccgtag gtcatcacca ggatcagcag gggtcgggcc ggcaagtcca
     9421 cgaacgcccg ggagccgatc gcttcctccg accggctccg gcagaagagg cgggtcagcc
     9481 ccacggcggc cgcgtaaatc gccagcagca cgagcccttc caggatgacg ggcagcagcg
     9541 cttggttcac agctccacct ccatgccatc gcgggccagg ctcgaacccg ggtacgcggc
     9601 caggagccgt cggtcgaggc gctccaggta ctcgtcccgg ctgtcggggg cgtggtggat
     9661 caccagcaac tgcccggcgc ccgctttccg cgccacctcg accgcctcct cccacgtgct
     9721 gtgcccccac cccttgaagg ccgggtacgt cgcgcggctg taatgcccgt cgaacagcag
     9781 cagccggcac ggcgccggct gccggcagaa ggccaggaac cgttcctgct cttccggcga
     9841 cgacacggac cattcgatgt ccgtcgcaaa gaccacggat gcgcccgtgg ccggctcatc
     9901 cagccggtag gcgacgcagc ccccgggatg gtgaacgggg caggaagaaa tccggaggcg
     9961 gccgaacgtg gccggagtct tttcaaaggt cacggccgcc tgcacgctgt ccatctgcac
    10021 cggccagaac ggctgcttca tcaactggga aaggatggat tccacggccg gcccgtcagt
    10081 gagcgacgag gccacgcgaa tggcgacccc cttgcggtac agcaggctga acgaaggcca
    10141 gccgacgata tgatcgaggt ggtaatgcgt caggagcagc agcacttcgc gaggcggctc
    10201 tgcgccccat gactcgccga gcgcccggat gccggtcccg gcgtccagga tgacggtctc
    10261 cccggcccgg ccctgcacca gcacggaggt cgtttctcca ccgtaccgca tgaactcgga
    10321 ccgcgccatc gagctcgtgc cccggacccc gccgagcgtg atcttcatag gttcatccct
    10381 tttccagcag attccacacg cccgcccagt ccgccggcgg gttcgcccgg tacagccggc
    10441 agcgctcggc gtaggcgcgc gacgccgggt cgtcggtcag ttgctcgaag accgccaggg
    10501 cgtccccgaa ccgcccctcg aagaagagct tcagcgcccg ctcgaaatcc gcgtgccagg
    10561 ccggggcggt ctcgccgggc aggcccgcgg gctcgtacac ggccacgggc tgctttcggc
    10621 ccaccacggt cagccgtccg agctggcgtc ccgcgaacgc gccgccggtc ccggtccacg
    10681 tggactcgga gaccatgaag taggtgccga aggccttgtt ggccccctcg aggcgcgagg
    10741 ccaggttggc ggcgtcgccc aggacggtat agttgaaccg gtcgcgggag cccatgttgc
    10801 cgaccgtcac gtcgccggtg ttgatgccga tgcgcatctt caccacggcg ccgtatttct
    10861 cctgccagtc ggcgcggcgt tccgccagcc gccgctggca gcggagcgcg gcccggcagg
    10921 cgcgcgccgc atggtcgggc tgcgccaccg gcgcgttcca gaacgcgacg atcgcgtcgc
    10981 cgatgaactt gtcgaggtac ccgccctcct ccttgatgat ctcgcccatc tcggagaggt
    11041 attcgttgag caggccgatg agctgggtcg ggtcgagccg ctcggagaac gatgagaatt
    11101 tctcgatgtc ggagaagaag atcgtcagct cgcgcttctc gccgccgagc gacagccgcg
    11161 acgggtcggc aaggagctgg tcgatcacgg tttcgcccag gtagaacttg aaggccgact
    11221 tgatgaacgc cttctgccgg ccctccgtgg catagttgac caccacggcg ccgaccatgg
    11281 ccagcagcac ggcgacctcg cccaccgcga ccggccacca gacgcccgcc cggtacgccg
    11341 ccagcccggc cagcggcggg atcggcagga agaccacgaa ggccgcgacg ttctgccaag
    11401 ccttccgccc gagggtcacg gcgacgccgc tcaacagggc cagcaggagc gtggccagcc
    11461 agaccatcca gggacgggca tcgcgcacga actgggcggc cagcaggttg tccagcatcg
    11521 tggtatggat
//
LOCUS       DAOOTK010000107        10826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_3983_length_10826_cov_4.555473, whole genome shotgun sequence.
ACCESSION   DAOOTK010000107 DAOOTK010000000
VERSION     DAOOTK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3983_length_10826_cov_4.555473"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7588)
                     /locus_tag="PK634_11110"
     CDS             complement(<1..7588)
                     /locus_tag="PK634_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW98324.1"
                     /translation="MNWRRTMAALLGVAALVFILALVRPGRLFTAADSQTGPGYPVRE
                     PAIAASRNGAASGSSEAGPSPAQRIAVWRRSFTPARAAPPGRPPEQKIELAAAVVDTS
                     GGAASVAPPKQQPTSRGTLPWMVQFEGPVREEWKAAVAGAGGVLRGYLPPYAFLVEMD
                     SASAGRVAALPGVRWVGEYKPDYKIQPFLRDLAARAATDPEVAEALAEPLSFTVQTMA
                     PEDASEVARALSAAGAEVKAVSPGRRWGLVRASAKVTEELLGNLALLGGVQWIEEYVP
                     PAWNNDFAARGDHINVTNVWAGHGLTGRKQVVAHADTGLDTGSLTNLHPDFAGRVKAI
                     YALGRPGDWSDPHGHGTHTAGSILGDGAGSTGQFRGVAYEASLVHQSVMDAYGGLGGL
                     PADLYDLYLQAYTNGARIHSDSWGAAVYGVYNEDAQSSDEFMWDHPQMLLVFSAGNEG
                     FDSPANGVIDPDSIGSPGTAKNMMTVGASENDRAPGSGGYSSYTFGQAWPADYTTDPI
                     NGDYISQSYDGAHQGLVAFSSRGPTDDGRFKPDIVAPGSDVISCRSQLPGAGVLWGTH
                     PNPKYAFSGGTSMATPLMAGAAALVRQYYMDRRGWALPSAALVKATLLNGARSLTPGQ
                     YGAHEFREIPPPPRPNNAEGWGQGDLENTLFPAAPASVLFHDVLALTTGGTNLYRLFL
                     ESNGTARLTLAWTDYPGTAGSGVKLVNDLDLLVLAPDGSVFYPNGRAAPDRTNNVEGV
                     DVPTPVSGVYTVRVSAFNVPSGPQNFALVFNGPWRPVDHTAPLPTTNTTAPYHLDFTV
                     TGLNAPDEAGMTVYWNTNGGARFATAAVARVSDTLYRASVPAHPSGARIYYYFEGTVE
                     GERRRHPTNAPASLHSFRVMDPVSLAVSGSPAACGTPSPAYGTHGFAPQASVTADVEA
                     FTAPAGLARWACTGWTGSGSVPVSGATNSVVFTILTGSALTWHWSGQYGLLQTSSVPG
                     ILNATSWWFAGASAQTLTAEPAFLLGATNHRFAGWNVDGARQPDAASPAVNPVTGLVM
                     SASRLAEAVYLPENRDSDVNGLPDWWELFYFGATGQGAGGDPDADTFPNGLEQQDQTD
                     PTDPASSPGFPVIAHNPLWDPQTRPAPWPVAAVVTDNYQVAEVRLWWQRNAEPWQDMA
                     LSPSAQPTRYTNAIPAPGTNGDEFAYYITAEDYAGLISTSAVTTFGVEYPVMGLAPTN
                     LGTVWMPPNSQTSRTVEVGNPGAASLTWNAQVSTGGVGWLTGAPTSGTVSAGSVTSVA
                     FSISSAGLTVGVSRTAQVVFRGNDPVAPTGIVPVVLRIGLAPTIAHTAPGPTTNTASP
                     YALDAVVTPSNLVDAAAAFVFWSTNGGAGFESAPLVKVTGDLYRAQVPAQPAPTRVEY
                     YLHAAATNSLVARLPTNAPAGLFSFDVTPPVSLLVTGAPPAGVVSPPYGIVEAASGQV
                     VEAYALADVPAGGGRYVCTGWLGSGSVPASGSGTAVTFTVREPSVLDWQWRREWSLAQ
                     TSMPAGLLSTTTWWSAGSAGQTVAAGASGLLGSTPYMFAGWFMDGQRQPAATGRVVNP
                     VAGLVMTGALQAAALYLPGVQDGDADGLPDWWELYFFGDTAQDEAGDFDGDTFRNLDE
                     WDDQSDPSDPASIPTAPVIQHTPLANPQTAPAPWPVTAVVTDNYTLDEVALEWSLNGG
                     GWSSAAMSPSVPVNLYTSAIPAPAVFGDHVEYFIRASDPAGHTAYSATSGVDVVYPVL
                     TADPTNLDLNWLMPGGTLGRTVQLENAGNSAVTWRMEVREAGLEDDMEAGAGGWTHSG
                     PGDLWRVSTNRSVSPRHAWYAGRSGDPGYDNGMDGRLVSAPLRVVQGSRLRLKYWIET
                     ELDPGDPPYAYDGGLVEISTNDGISFFAVAPVDGYPALARGLYGSPFAPGTPFFAGTG
                     GWQAAEFDLSAYAGREVRLACRFGSDESFAEGEGWYVDDIALTPATGTNDWLDIAPTS
                     GVLAAVSGEAASVGLDAAGLAAGDSRGVVLALLSDDPVNPEISIPLSIFIGAPVTLEH
                     TPLSNATNTTDGYAVSAEATPQGPLPTNGLSVRALWSASSALGPFATNDLSHAAGNVW
                     TGAIPAQPRGTRLYYFLHAQAGAAYALHPAGAPAAVHTFQITAPADLLVQGQPAAYGS
                     PEPPYGTSTLAIGTPVSAAAPDVLAGGTWRTCTGWVGSGSVPAQGDTNHVSFTIETNS
                     TLQWKWSTRYRLAETSTPAGIILRTTWWPVGAVVSTAVAEAGVTWEETAYRFAGWQLD
                     GSRWPASTGRAAFVVTGLAMFSPHQAGALYLPAEQDDDGDGLADWWEHFEFGTDAADA
                     GEDPDGDGYSNYTEFKEETNPNDPSSTPAPPFLAHTPLADPQTNPAPWSVEAVATDNF
                     ALASTPLSGTALWFDGEDDYVLRNPVTRFPSNEITVEFWMRSSDRSDNGTPVSYAVSG
                     DFGDANEFTLYNYNTFLLYRGAAYTNTGIAVADGAWHHVAVSWRSADGAARLFKDGVE
                     AWSGEIAAGQDLRPGGTLVFGQDQDSLGGGFTAGDAFSGLLDEVRVWSV"
     gene            complement(7637..8635)
                     /locus_tag="PK634_11115"
     CDS             complement(7637..8635)
                     /locus_tag="PK634_11115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOW98325.1"
                     /translation="MNRKSAIHAAWLVVCGLLLLGAGPARGQETDWSADLNRILSRLQ
                     SMGHGYYSAAEWQDVFQQIDEVTDRATRAGAWETLVELNAIKAMVLSDMRGAHDQALK
                     VVRAARKRLARHKPKNMARLYVREAAVLSKLGDEAAIGRLIDEFRASEFFDPEQYAYS
                     VQDGRDTPMAIVRPNPKGSDSISITAMEKYRQQARFAAGRPFPEFEGLTPDGRRVSLS
                     DLRGKVVLVDLWFRGSLPWQREVPNLVRLYQEYQPRGFEILGVNLERNPGGLAEFLAG
                     KGITWTQIVGPTDLPRQLGVFGDTARFLLDPNGRIIGRDLKGSDLSASIRQALGVP"
     gene            complement(8699..9184)
                     /locus_tag="PK634_11120"
     CDS             complement(8699..9184)
                     /locus_tag="PK634_11120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOW98326.1"
                     /translation="MRDGWLPWTRSCFVCGEENPHGLRLRSRLENGRVVLEYTTRRTD
                     VGYRHIVHGGIAATLLDEVMTWAAIVATRKVCVAAELTTRLKAPIGVGQRIRVEGRVT
                     RAGARLCLAEGSIRDEQGRELMSAAGKYMPMPAAMAALSEKDFVHHPEALAPGDIVGN
                     G"
     gene            complement(9201..>10826)
                     /locus_tag="PK634_11125"
     CDS             complement(9201..>10826)
                     /locus_tag="PK634_11125"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOW98327.1"
                     /translation="TITIQNYFRMYKKLAGMTGTAETEADEFHQIYKLDVVVIPTNRP
                     VRRVDNNDLIYKTQREKFNAIIAEIEECYKQGQPVLVGTISVETSELISRMLRRTGIP
                     HNVLNAKNHQREAEIVALAGQKGAVTIATNMAGRGTDIKLGEGVVWLDREIVKSARPL
                     DEKVDGQKLRQLLLEKPCGLHVIGSERHESRRIDRQLRGRCARQGDPGSSRFYVSLED
                     DLMRLFGSDRISRIMERLGIQEGEVLEHPWLNRSIETAQRRVEQHNFSIRKRTLEYDD
                     VMNKQREIIYGFRSEVVADPEPRKHLFSVAEDVVLARAEEMFSEEDEKARHEFLGWVN
                     ATFPVGLKFEELAGVPDADALTAKVIERVRKAYDVKARLENPDALGNMERFIIVQAVD
                     THWQEYLRNMDTLRQGVGLRAYGQRDPLVEYKHEAYSMFSDLMDRIKADISTRMFRSA
                     TSAEAFRSFAATLSQVYVHDEVSTLGTHRAPAPARAPAGEAASPPGAEAAMQAALHAK
                     GETFKREEAKVGRNDPCPCGSGKKYKKCHGAGK"
BASE COUNT         1595 a         3832 c         3719 g         1680 t
ORIGIN      
        1 cgacggacca cacgcggacc tcgtccaaca ggccgctgaa cgcgtcgccg gccgtgaacc
       61 cgcccccgag gctgtcctgg tcctggccga agaccagcgt gccgccgggc cgcaggtcct
      121 ggccggcggc gatctcgccg gaccaggctt cgacgccgtc cttgaaaagc cgggccgcgc
      181 cgtccgcgct ccgccacgag acggcgacgt ggtgccaggc gccgtcggcc acggcgatcc
      241 cggtgttcgt atacgcggct ccccggtaca gcaggaacgt gttgtaattg tagagcgtga
      301 attcgttcgc gtcgccgaag tcgccgctga ccgcgtacga taccggcgtg ccgttgtcgg
      361 aacggtcgga cgagcgcatc cagaattcga ccgtgatctc gttggacggg aaacgcgtca
      421 cgggattgcg caggacgtag tcgtcctcgc cgtcgaacca gagggccgtg ccggacagcg
      481 gcgtggaagc cagcgcaaag ttatccgtcg ccacggcctc gaccgaccac ggcgcgggat
      541 tcgtctgggg atcggccagc ggcgtgtgcg caaggaacgg cggcgcgggc gtggaggaag
      601 gatcgttcgg gttcgtttcc tccttgaact ccgtgtagtt cgaatagccg tcgccatccg
      661 gatcctcccc cgcgtccgcg gcgtcggtcc cgaactcgaa atgctcccac cagtcggcca
      721 acccgtctcc gtcgtcgtcc tgctcggccg gcaggtagag cgcgcccgcc tggtggggcg
      781 agaacatcgc caggcccgtc acgacgaagg ccgcccgccc ggtcgacgcg ggccagcggc
      841 tgccgtccag ttgccagccg gcaaagcgat aggcggtttc ctcccatgtc acccccgcct
      901 ccgccacggc ggtggacacg accgcgccca ccggccacca ggtggtccgc aggatgatgc
      961 cggcgggcgt ggacgtctcc gccaggcggt accgcgtgct ccatttccat tgcagcgtcg
     1021 agttcgtttc aatcgtgaag ctcacgtgat tcgtgtcgcc ctgcgcgggc acgctgcccg
     1081 agccgaccca cccggtgcac gtccgccacg ttccgcccgc gagcacgtcc ggcgcggcgg
     1141 cgctcaccgg cgtgccgatc gccagcgtcg aggttccata gggcggctcg gggctcccgt
     1201 aggcggccgg ctgcccctgt accagcaggt cggcgggagc cgtgatctgg aacgtgtgca
     1261 cggcggccgg cgccccggcg ggatgcaggg cgtatgccgc gccggcctgc gcgtgcagga
     1321 agtagtacag ccgcgtgccg cgcggctgcg ccgggatggc gcccgtccat acgttgccgg
     1381 ccgcgtgcga gaggtcgttc gtcgcgaagg gcccgagcgc gctcgacgcg ctccatagcg
     1441 cccgcacgct caagccgttg gtcggcagcg ggccctgcgg cgtcgcctcg gcgctcaccg
     1501 cgtaaccgtc cgtcgtgttc gtcgcgttcg acaggggcgt gtgctcgagc gtgaccggcg
     1561 cgccgatgaa tatcgagagc ggtatggaga tttccgggtt caccggatcg tcggacagca
     1621 acgccagcac gacgccgcgg ctgtcccccg ccgccaggcc cgccgcatca agaccgacgc
     1681 tcgccgcttc gccggaaacc gcggccagga cacccgacgt cggggcgatg tcgagccagt
     1741 cgttcgttcc cgtcgccggc gtcagggcga tgtcgtccac gtaccacccc tcgccctcgg
     1801 cgaagctctc gtccgaaccg aaccggcacg ccagccgcac ctcgcggccg gcgtaggcgg
     1861 acagatcgaa ttccgccgcc tgccagccgc ccgtgccggc gaagaagggc gtgcccggcg
     1921 caaagggcga tccgtacaac ccgcgggcca gcgccgggta tccgtcaacc ggcgccacgg
     1981 cgaaaaacga aatcccgtcg ttcgtggaaa tctcgaccag cccgccgtcg taggcatagg
     2041 gcggatcgcc gggatccagt tcggtttcga tccagtactt gagccggaga cgcgagccct
     2101 gcaccacccg caacggcgcg ctgaccagcc ggccatccat gccgttgtca tagccgggat
     2161 cgcccgagcg cccggcatac cacgcatgac gcggcgacac gctgcggttc gtcgacaccc
     2221 gccacaggtc gcccggaccg gaatgcgtcc acccgcccgc gccggcctcc atgtcgtctt
     2281 ccagcccggc ttcccggact tccatgcgcc aggtcacggc ggagttcccg gcgttttcca
     2341 gttgcacggt ccgccccagc gtgccgccgg gcatcagcca gttgagatcg aggttcgtcg
     2401 gatcggcggt caggacagga tacaccacgt ccaccccgct ggtggcgctg taggccgtgt
     2461 ggcccgcggg atcggaggcc cggatgaaat actccacgtg gtcgccgaag accgccggcg
     2521 ccgggatcgc gctggtgtag aggttgaccg gcacggacgg cgacatcgcc gcgctgctcc
     2581 agccgccccc gttcaaagac cattcaaggg ccacttcgtc cagcgtgtaa ttgtccgtca
     2641 cgacagcggt cacgggccag ggcgcgggcg cggtctgcgg attggccagc ggggtatgct
     2701 ggatgaccgg cgccgtgggg atggaggccg ggtcggacgg gtcgctctga tcgtcccatt
     2761 cgtccaggtt cctgaacgtg tcgccgtcga aatcgccggc ctcatcctgc gcggtgtccc
     2821 cgaagaaata cagctcccac cagtccggca ggccgtccgc atcgccgtcc tgcacccccg
     2881 gcaggtataa cgccgcggcc tggagcgccc cggtcatgac caggcccgcc acgggattca
     2941 ccacgcggcc ggtcgccgcc ggctggcgtt gcccgtccat gaaccacccg gcgaacatgt
     3001 acggggtcga gccgagcagg cccgacgccc cggcggccac ggtctgcccg gcggagcccg
     3061 cgctccacca ggtcgtcgtc gaaagcaatc ccgccggcat ggacgtctgc gcgagcgacc
     3121 actcccgccg ccattgccag tccaggaccg acggctcccg cacggtgaac gtgaccgccg
     3181 tcccgctgcc ggaggccggg acgctgccgc tcccgagcca accggtgcag acataccgcc
     3241 cgcccccggc ggggacatcc gccagcgcat acgcttccac gacctgaccc gacgcggcct
     3301 ccacgatccc gtagggcggg ctcacgacgc cggcgggcgg agcgcccgtc acgaggagag
     3361 aaacgggcgg cgtcacgtcg aacgagaaca gtccggccgg cgcgttggtc gggagccggg
     3421 cgaccaggct gttggtcgcc gcggcgtgca ggtagtactc cacccgcgtc ggcgcgggct
     3481 gggccgggac ctgcgcgcga tacaggtcgc ccgtgacctt caccagcggc gcggactcga
     3541 aaccggcccc gccgttggtg ctccagaaca cgaaggccgc ggcggcatcc accaggttgg
     3601 acggtgtcac gacggcgtcc agcgcatagg gcgaggccgt gttggtggtc gggcccggcg
     3661 cggtgtgcgc gatggtggga gccaggccga tgcgcagcac gaccggcacg atgccggtcg
     3721 gcgcgaccgg gtcgtttccg cggaacacaa cctgcgcggt gcggctgacg ccgacggtca
     3781 ggccggcgct gctgatggag aacgcgacgg acgtgaccga gccggccgac acggttccgc
     3841 tcgtcggggc gccggtcagc cagccgaccc cgccggtcga gacctgcgcg ttccacgtca
     3901 atgacgccgc gccgggattg cccacctcca cggttcggct ggtctggctg ttcggcggca
     3961 tccagaccgt tcccaggttc gtcggcgcca atcccatcac gggatactcg acgccgaatg
     4021 tcgtcaccgc gctcgtgctg atcaggccgg cgtagtcttc cgccgtgatg tagtacgcga
     4081 actcgtcgcc attcgtcccc ggcgccggga tcgcgttggt gtaacgcgtc ggctgcgcgg
     4141 agggcgacag cgccatgtcc tgccagggct ccgcgttccg ctgccaccag agccggacct
     4201 cggccacctg gtagttgtcg gtcacgacgg cggccaccgg ccagggcgcg ggccgtgtct
     4261 gcgggtccca caggggattg tgggcgatca ccgggaagcc gggcgacgaa gccggatcgg
     4321 tgggatcggt ctggtcctgc tgctccaacc cgttcgggaa tgtgtcggcg tcgggatcgc
     4381 cccccgcgcc ctgccccgtg gcgccgaagt aaaacagctc ccaccagtcc ggcagcccgt
     4441 tgacgtccga gtcccggttc tcgggcaggt agacggcttc ggccaggcgc gacgcggaca
     4501 tgaccaggcc cgtgacggga ttgaccgccg gcgaggccgc atccggttgc cgcgcgccgt
     4561 ccacgttcca gcccgcgaaa cgatgattgg tcgcgccgag caggaaggcg ggctccgcgg
     4621 tcagcgtctg tgcggacgcg ccggcgaacc accaactggt ggcattcagg atgcccggca
     4681 cgctggaagt ctggagcagc ccgtactggc ccgaccagtg ccaggtcagc gcggaacccg
     4741 taaggatcgt gaacacgacg gagttggtcg ccccggagac aggcacgctg cccgagcctg
     4801 tccaaccggt gcaggcccat cgcgccaggc ccgccggcgc ggtgaacgcc tcgacgtccg
     4861 ccgtcacgga agcctgcggg gcaaaaccgt gcgtcccgta cgccgggctc ggcgtcccgc
     4921 aggccgccgg cgatccggaa accgcgagcg acacgggatc catcacgcgg aaggaatgga
     4981 gcgaggctgg ggcgttcgtc ggatgcctcc gccgttcgcc ctcgaccgtc ccctcgaaat
     5041 agtaatagat ccgggcgccg gacgggtggg cgggaaccga ggccctgtac agcgtatcgc
     5101 tcacgcgcgc gacggcggcc gtggcgaacc gcgcgccccc gttcgtgttc cagtacaccg
     5161 tcatgccagc ctcgtcgggc gcgttcaggc ccgtcaccgt gaaatcgagg tggtagggcg
     5221 ccgtggtgtt agtggtcggc agcggcgccg tgtgatccac cggacgccag gggccgttga
     5281 acaccagcgc aaaattctgg ggcccgctcg gcacgttgaa ggcgctcacc cgcaccgtgt
     5341 acacgcccga gacgggcgtc ggcacgtcca ccccctcgac gttgttcgtg cgatcgggcg
     5401 cggcccggcc gttgggataa aacaccgagc cgtccggcgc gaggacgagc aggtccaggt
     5461 cgttgaccag tttcacgccg ctgcccgcgg tgcccgggta atccgtccag gccagggtca
     5521 ggcgcgccgt gccgttgctc tcgaggaaca gccggtagag gttcgtgccg ccggtcgtca
     5581 gcgccaggac gtcgtggaag aggaccgacg ctggcgcagc ggggaacagc gtgttctcca
     5641 ggtcgccctg cccccacccc tcggcgttgt tcggccgcgg cggcggcggg atctcgcgga
     5701 actcgtgggc gccgtactgg cccggggtca gcgaccgcgc cccgttcagc agcgtggcct
     5761 tgaccagcgc ggcgctcggc agggcccagc cgcgccggtc catgtaatac tggcgcacga
     5821 gcgccgccgc gccggccatg agcggtgtgg ccatgctcgt gcctccgctg aaggcgtact
     5881 tcgggttggg atgcgttccc cacagcacgc ccgcccccgg gagctgcgac cggcacgaga
     5941 tcacgtcgga gcccggtgcg acgatgtccg gcttgaatcg cccgtcgtcg gtcggcccgc
     6001 ggctggaaaa cgccaccagc ccctggtgcg cgccatcgta actctgcgag atgtaatcgc
     6061 cgttgatggg atcggtcgtg tagtccgccg gccaggcctg gccgaaggtg taggaactgt
     6121 acccgcccga gccgggcgcc cggtcgttct cgctggcgcc gacggtcatc atgttcttgg
     6181 ccgtgccggg cgacccgatg gagtcgggat cgatcacgcc gttggcgggc gagtcgaatc
     6241 cctcgttgcc cgcggagaag accagcagca tctgcgggtg gtcccacatg aactcgtccg
     6301 aggactgcgc gtcctcgttg tacactccgt acaccgccgc gccccagctg tcggaatgaa
     6361 tgcgcgcgcc gttggtgtac gcctgcaggt acaggtcgta gagatcggcc ggcaggccgc
     6421 cgagcccgcc gtacgcgtcc atgaccgact ggtgcaccag cgacgcctcg tacgccacgc
     6481 cgcggaactg ccccgtgctc cccgcgccgt cgccgaggat cgaccccgcc gtgtgcgtgc
     6541 cgtgcccgtg cgggtcgctc cagtcgccgg gccggcccag cgcatagatg gccttcaccc
     6601 ggcccgcgaa atcgggatgc aggttggtca gggatcccgt gtcgagcccg gtatccgcgt
     6661 gcgccacgac ctgcttccgg ccggtcagcc cgtgccccgc ccagacattg gtgacgttga
     6721 tatgatcccc gcgcgccgcg aaatcgttgt tccacgccgg gggcacgtat tcctcgatcc
     6781 actgcacccc gccgagcagg gccaggttgc ccagcagttc ctccgtcacc ttcgcgctcg
     6841 cgcggaccag gccccagcgc cggccgggac tgacggcctt cacctcggcg cccgccgccg
     6901 acagagcgcg cgcgacctcg gacgcgtctt ccggggccat cgtctgcacc gtgaacgaca
     6961 acggctccgc gagcgcctcc gccacctccg ggtccgtcgc cgcccgcgcc gcaagatccc
     7021 ggaggaaagg ctggatcttg tagtcgggct tgtactcgcc cacccagcgg acgcccggca
     7081 gggcggccac acgccccgcg gacgcggagt ccatctccac gagaaaggcg tacggcggca
     7141 agtacccgcg cagaacaccg cccgcgcccg cgacggccgc cttccactcc tcgcggaccg
     7201 ggccctcgaa ctggaccatc cagggaaggg tgcctcgcga ggtcggttgc tgcttcggcg
     7261 gggccacgga agcggccccg cccgacgtgt ccaccacggc ggcggccaat tcgatcttct
     7321 gttccggcgg ccgtccgggc ggcgcggcgc gcgccggcgt aaaggaccga cgccagaccg
     7381 cgatacgctg cgcgggcgac gggccggcct ccgaggagcc ggacgccgct ccgttcctgg
     7441 acgcggcgat ggccggctcg cgaacgggat agccgggtcc ggtttgtgaa tccgcagcag
     7501 tgaacaaccg cccgggccgg accagcgcca ggatgaaaac gagagcggcc accccgagca
     7561 aggccgccat cgttctgcgc cagttcatcg gttacaacgt accaggatta gcgggcccgg
     7621 taaacgcgga cggccgtcag ggcacgccca gcgcctgccg gatcgaggcc gacaggtcgg
     7681 agcccttcag gtcccggccg atgatccggc cgttcggatc cagcaggaac cgggccgtgt
     7741 ccccgaacac gccgagttgg cggggcaggt ccgtcgggcc gacgatctgc gtccaggtga
     7801 tgcctttccc ggccaggaac tcggccagcc cgcccgggtt acgctcgagg ttgacgccga
     7861 ggatctcgaa tccgcgcggc tggtactcct ggtagagccg gaccaagttc ggcacttccc
     7921 gctgccaggg cagcgaaccg cggaaccaca ggtccaccag cacgaccttg cctcggaggt
     7981 cggacaggct gacccggcgg ccgtccggcg tgaggccttc gaattccggg aacgggcggc
     8041 cggcggcgaa gcgcgcttgc tgccggtact tctccattgc cgtgatggaa atggagtcgc
     8101 tgcctttcgg gttgggccgg acgatcgcca tgggcgtgtc ccggccgtcc tgcacggagt
     8161 aggcgtactg ttccggatcg aagaattcgc tcgcgcggaa ttcatcgatc agccggccga
     8221 tcgcggcctc gtcgccgagc ttggacagca cggccgcctc gcgcacgtag agccgcgcca
     8281 tgttctttgg cttgtgccgc gccagcctct tgcgcgcggc ccggacgacc ttcaacgcct
     8341 ggtcgtgggc gccccgcatg tcgctcaaga ccattgcctt gatcgcgttc aactcgacca
     8401 gcgtctccca ggcgccggcc cgggtggccc ggtcggtgac ctcgtcgatc tgctggaata
     8461 cgtcctgcca ttccgccgcg gaatagtacc cgtgccccat ggattgcagg cggctcaaga
     8521 tgcggttcag gtcggccgac cagtccgtct cctgcccgcg cgccggcccg gccccgagca
     8581 gcagcaggcc gcaaaccacc agccacgccg catgaatagc gctttttcga ttcatggcat
     8641 tcccctcttt tggctgaagt atgaaccttt ttgccccggg tttgaatgaa aataaccgtc
     8701 agccattccc gacaatatcg ccgggggcca gggcttccgg gtgatgcacg aagtctttct
     8761 cgctcaaggc ggccatggcc gcgggcatgg gcatgtactt gccggctgcc gacatcagtt
     8821 cccggccctg ctcgtcgcgg atggagccct cggccaggca cagccgggcg cccgcccgcg
     8881 tcacccggcc ctcgacgcga atcctctgcc cgacgccgat cggcgccttc aggcgcgtcg
     8941 tcagttcggc cgccacgcag accttccggg tggcgacaat ggccgcccac gtcatgacct
     9001 cgtccaggag cgtcgcggca atgccgccgt gcacgatatg tcggtacccg acgtcggtcc
     9061 gccgcgtggt gtattccagc acgacccggc cgttttccag ccgggaccga aggcgcaggc
     9121 cgtggggatt ctcctcgcca cacacgaaac acgatcgggt ccagggcagc cagccgtctc
     9181 tcatggcctc ccccggcggg ttacttcccc gccccatggc acttcttata cttcttgccg
     9241 ctgccgcacg ggcacgggtc gttgcgccca accttggcct cctcgcgctt gaaggtctcg
     9301 cccttggcgt ggagcgcggc ctgcatcgcc gcctcggcgc caggcggaga ggcggcctcg
     9361 ccggccgggg cgcgggcggg cgcgggcgcc cggtgcgtgc ccagcgtgga aacctcgtcg
     9421 tggacgtaga cctgcgagag ggtggccgcg aagctgcgga aggcctcggc cgacgtcgcg
     9481 gagcggaaca tccgcgtgga gatgtccgcc ttgatgcggt ccatcaggtc ggagaacatg
     9541 gaataggcct cgtgcttgta ctcgacgagc ggatcgcgct gcccgtaggc ccgcagcccg
     9601 acgccctggc gcagggtgtc catgttgcgc aggtattcct gccagtgggt gtccacggcc
     9661 tggacgatga tgaagcgctc catgttgccg agggcgtcgg gattctccag gcgcgccttg
     9721 acgtcgtagg ccttgcgcac ccgctcgatg accttggccg tcagggcgtc cgcgtcgggc
     9781 acgccggcca gctcttcgaa cttgaggccg accgggaacg tggcgttgac ccagccgagg
     9841 aactcgtggc gcgccttctc gtcctcctcc gaaaacatct cctccgcgcg cgcgagcacg
     9901 acgtcctcgg ccacgctgaa caggtgcttg cgcggctcgg gatcggcgac gacctcgctg
     9961 cggaagccgt agatgatctc gcgctgcttg ttcatcacgt cgtcgtactc cagcgtgcgc
    10021 ttgcggatgg agaagttgtg ctgctcgacg cggcgttggg cggtctcgat ggagcggttg
    10081 agccaggggt gttccaggac ctcgccctcc tggatgccga gccgctccat gatccgcgag
    10141 atgcggtcgg agccgaacag gcgcatgagg tcgtcttcca gcgatacgta gaagcgcgag
    10201 gagccggggt cgccctgccg ggcgcagcgg ccacggagct ggcggtcgat gcggcgggac
    10261 tcgtggcgct cggacccgat cacgtggagc ccgcagggtt tctcgagcag cagctggcgg
    10321 agcttctggc cgtccacctt ctcgtccagc gggcgggcgg acttgacgat ttcgcggtcg
    10381 agccagacga cgccctcgcc cagcttgatg tccgtgccgc gcccggccat gttcgtggcg
    10441 atggtcacgg cgcccttctg gccggcgagc gcgacgatct cggcctcgcg ctggtggttc
    10501 ttggcgttga gcacgttgtg cgggatgccg gtccgccgga gcatgcggct gatcagctcc
    10561 gaggtctcga cggagatcgt gccgacgaga acgggctggc cctgcttgta gcactcttcg
    10621 atctcggcga tgatggcgtt gaacttctcg cgctgggtct tgtagatgag gtcgttgttg
    10681 tccacgcgcc gcacggggcg gttggtgggg atgacgacga cgtcgagttt gtagatctgg
    10741 tggaactcgt cggcctcggt ctccgcggtg ccggtcatgc cggcgagctt cttgtacatg
    10801 cggaagtagt tctggatcgt gatcgt
//
LOCUS       DAOOTK010000108        10731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4024_length_10731_cov_5.364556, whole genome shotgun sequence.
ACCESSION   DAOOTK010000108 DAOOTK010000000
VERSION     DAOOTK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4024_length_10731_cov_5.364556"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PK634_11130"
     CDS             complement(<1..606)
                     /locus_tag="PK634_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW98328.1"
                     /translation="MSRHAQSVRRRFDAAADCYESRSSVQARVADRLMDLWPDAGSIH
                     RILEAGCGTGLLTRRLAARFPGASIEALDQAPRMIEAARRRCPGLTWHATDLLEFHSD
                     LRYDLIASNCSLHWVEPLGNGFRILRKLLAPDGRLVFSIMLDGTLGELRAARLIAAPA
                     KPPLGRLPSLPEVRAALESGGWRVERFHEEQLEERHASARDF"
     gene            complement(603..1337)
                     /locus_tag="PK634_11135"
     CDS             complement(603..1337)
                     /locus_tag="PK634_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOW98329.1"
                     /translation="MKAPVLLVSGWAHPAAALEPLAHRLAERPVRLLGLEAIHVAAPR
                     SGLSGYASGLLQEIEAQEEPVILAGWSMGGMVALEAAAAAPGRIAALILISAAPRLAA
                     DQDFPEGVPPRILQAMTLSFRRTPERTLRDFFINAASPSEPDGETAAQFAHRALESGT
                     EALAEQLEYLVRTDLRPACARLRMPRLLLHGTRDRIIPAGAAAWLRAHSAGTEGEVRE
                     GAGHRLPLDHPDWTAERIVRFLEGAA"
     gene            complement(1334..2503)
                     /gene="bioF"
                     /locus_tag="PK634_11140"
     CDS             complement(1334..2503)
                     /gene="bioF"
                     /locus_tag="PK634_11140"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOW98330.1"
                     /translation="MQGENWLEDGLAELRAGDLERRLTAFPATGGRITVGGRTLLNLS
                     SNDYLGLACHPRVVEAAVKALHEFGAGATASRLITGTLPLHEELEQRLAALKGYPAAL
                     VFGSGYLANAGTIPALVGPDDLVLADRLAHASLLDAAVLSRARLLRFRHNDALHLDEL
                     LSAHSARRRLVLTESVFSMDGDLAPLPDLAAVAARHEALMLVDEAHATGVFGPGGAGL
                     ISEHGLADRVAVSMGTLSKALGSYGGFVACAENLRAWLVNKARAFIYTTAPPPAVLGA
                     ALGALMVLSKRPEMGQELLARAAGFRDTLKAAGLDTLNSASQIVPLMVGDNAKALALS
                     RRLKDDGILAVAIRPPTVPPGTARLRFSISLAHQPADLAQAAQTIIAAACAENVA"
     gene            2564..3541
                     /gene="bioB"
                     /locus_tag="PK634_11145"
     CDS             2564..3541
                     /gene="bioB"
                     /locus_tag="PK634_11145"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOW98331.1"
                     /translation="MNWLDCGQRVKAGGALTREEALAVLQSSDDEILEVVQAAFLARR
                     QHFGRDVNLHILRNAQSGLCGENCAFCSQSASSAAEIERYPLQSIEEIVAGALDAQRA
                     GAVKYCVVTSGRAPAADTLDTFCEAARLIRRATSLHLCVSPGLLTEGQARRLAGAGVH
                     RVNHNLETARRYFPAICTTHSYEDRVATVRAVKAAGMEACCGGILGLGETLEDRVDLA
                     FALRELGVDSIPVNFFNPRPGTPLADQPPLGARDGLRGLAMFRLVNPTRDIRAAGGRE
                     ACLGALQPLALYAANSIFTSGYLTTPGQGRPADIRMLEEAGFRPAKLEM"
     gene            3556..4326
                     /locus_tag="PK634_11150"
     CDS             3556..4326
                     /locus_tag="PK634_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW98332.1"
                     /translation="MDVQEITIRSALVRSRIPGVEYVINPYLGCGHGCRYCYAVFMTK
                     HSRHHAGAAWGSFVEAKTNIAAILRDELRRKRKTATAMLSSVCDPYQPAERRYKLTRA
                     CLEALRDFGWGMEILTRSPLVLRDLDILQSGSRVSVGFSVPTDDDRVRRVTEPNAPPI
                     GSRIATLRRLKEAGIPTWAFIAPMLPMNPERLAELLAPVIDSVMMSGLNYVGRVAGLF
                     RRLGWASALTRGYAREMRERLGRSLDAKMLHAAPPEEG"
     gene            complement(4552..5592)
                     /gene="nadA"
                     /locus_tag="PK634_11155"
     CDS             complement(4552..5592)
                     /gene="nadA"
                     /locus_tag="PK634_11155"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOW98333.1"
                     /translation="MSASEMYEKMKARLGHVVPDAELRYKAGLADEIMRLKQERNAVI
                     LAHNYMEPALYHSVPDFTGDSLELSRKAAQSDKDVIVFCGVRFMAETAKILNPRRTVL
                     LPAQRAGCSLAESITAEDVRKLKQQFPGVPVVTYVNTYADVKAESDICCTSGNAAAVV
                     NSLKADRVIFLPDEFLARNVAKETGKRIIFPARKSRRKGGAPENLDYQMIGWTGSCEV
                     HDRFTVEDVKNARRQFPDVVVLAHPECRPEVVRASDFAGSTMAMVRYVKESAAQRYLL
                     LTECSMGDNIAAENPDKEMVRICSIRCPHMNEITLEDTLNSLKLNRYVVEVPEDIRVR
                     ALRAVERMISIG"
     gene            5711..6979
                     /locus_tag="PK634_11160"
     CDS             5711..6979
                     /locus_tag="PK634_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98334.1"
                     /translation="MSNEKPSDPVPPSPEIPESGPRHPRPARREQAACDALRTFVRDL
                     HQDRFGTAPPRLEEGELVLKMKARPGEDWALRFDPPLSEQLETQLDDWQASRDIYREG
                     RVYCFRCDTSECEHARPFSPLEVFKEYAPNGIPEWHELAQALLAGGDERAERLYREGG
                     GLVALFQPGRLLRGKQLSSFGRSSRTYAILAQVASGFFPAPEGLSPEGGQGSRLAISF
                     QAVEVRGPRGERILRLNPVTRGDVAGLRESLTSGWQPALYRAWKTAEQEMEYLERAAR
                     EAGAGRDGARVHEVMRRLPGIMRRLAESLERGGRQESRRTHHVERRRHEHRPVHKALE
                     DAQAAAPGLVFDDEKAGTTVACGPQSRAHAFNRDGRHVTSFVLRPQAIDLRVRTRRWR
                     VLAPDEVAEFKRRIAQYVPAQKDMAPPLSR"
     gene            complement(6946..7638)
                     /locus_tag="PK634_11165"
     CDS             complement(6946..7638)
                     /locus_tag="PK634_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOW98335.1"
                     /translation="MLTSLAAILTSGTLLFLDAPAADSKRVPPNYEFEKMPHRNTLVD
                     NVTFVAFDTETTGFSPAGDRIVEIGAVKFRNGEIIEEKSWLINPGRWIPPWVIEVHGI
                     TNEDVADKPSFKQVYPEFVDFVQGSVLMAHNARFDVSFMAAEIRRSELPLLNNLVVDS
                     LPLFRRWFPSAKSYTLSDVAHYTKVDTGVLHRALADTVYMVLIFEKGLSKRSASLRLG
                     ELYRESGGAMSF"
     gene            7968..9365
                     /gene="cysS"
                     /locus_tag="PK634_11170"
     CDS             7968..9365
                     /gene="cysS"
                     /locus_tag="PK634_11170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOW98336.1"
                     /translation="MALRFYNTLTRQLDTFEPLEEGRVRLYTCGPTVYHYAHIGNFRS
                     YMFEDLLRRYLRFKGFRVTQVMNLTDVDDKTIRGARERGVSLNEYTQQYKDTFFEDLK
                     TLNIEPAEHYPAATDHVPEMIALIERLMEKGVAYRSDDGSVYFSIAKFPAYGRLAHLD
                     LSGLRAGARVAQDEYEKDNVADFALWKAWDENDGEVAWDSPWGRGRPGWHIECSAMSM
                     KYLGESFDIHTGGVDNMFPHHEDEIAQSEAATGKPFVKYWLHCAHLIVDGRKMSKSLG
                     NFFTLRDVIGRGFSGREVRYVLMATHYRQWLNFTFDTLRAARAALDRLDEFRARLEKT
                     SAGAAAGPAPAWADEALDRFAAAMDEDLNISGALGALFDMIHAGNKALDGGTVDAPTA
                     GGALATLRALDRVLGLLQETGETADAAARELAALRLKAREAKNWPEADRLRKELLALG
                     WLVKDTPQGPELKKA"
     gene            9397..10035
                     /gene="tmk"
                     /locus_tag="PK634_11175"
     CDS             9397..10035
                     /gene="tmk"
                     /locus_tag="PK634_11175"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOW98337.1"
                     /translation="MRGKFITFEGPEGGGKTTQARRLIARLEKDGHPVLYAREPGGTP
                     TGEAIRDILQYDKAGEPICRETEVLLFAASRAQLVRHVILPALEAGRQVVCDRFADST
                     TAYQGFGRGFPVEQMLTINEFAINGAHPDLTLLLDIDVGMGFERLTRRHRETASTWDR
                     IEREERAFHEKVRQGYLELARRWPARFRIVNAGRDPDAVEQEIWDHVRRLLA"
     gene            10013..>10731
                     /locus_tag="PK634_11180"
     CDS             10013..>10731
                     /locus_tag="PK634_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98338.1"
                     /translation="MSAGSSLDRFAELFASLRQGHAAGRLAHAYLVVGAPRGNALALS
                     EALLQLLFCAGEPRPCGECAECRRVRERAHPDILWIEPESKSRRIKIERIREELIPRL
                     SQGSYGGGWKAGVLIQADRLTGEAANAFLKMLEEPPPSSLILLLTDSPQELLPTIVSR
                     CHRLLLSAEPAARTEPWYASLLDILRSDAPSDPLQALGRVARMKALLESVRAAAESEA
                     PPAAAGEAEPEEDVEDARVRTR"
BASE COUNT         1839 a         3466 c         3652 g         1774 t
ORIGIN      
        1 gaaatcccgg gccgaggcat ggcgctcctc cagctgctcc tcgtgaaatc gctcgacccg
       61 ccagccgccg gattccaggg ccgcccgcac ttccgggagg gatggcagtc gtcccagcgg
      121 cggtttcgcg ggggccgcga tcagccgcgc tgcccgcagt tcccccagcg tgccgtcgag
      181 catgatggag aagaccagcc gcccgtccgg cgccagcagt ttccggagaa tccgaaaacc
      241 gttcccgagc ggttcgaccc agtgcaggga gcaattggac gcgatgaggt cgtaccgcaa
      301 gtcgctgtgg aattccagaa ggtcggtcgc gtgccaggtg aggcccggac aacggcgccg
      361 ggcggcctcg atcatccgcg gggcctggtc gagcgcctcg atggatgcgc cggggaaccg
      421 cgcggccagg cggcgcgtca gcagtcccgt cccgcacccg gcttccagga tccgatggat
      481 cgaaccggcg tcgggccaca ggtccatcaa ccggtcggcc acgcgcgcct gcacggagga
      541 ccgggattcg tagcaatccg cggcggcgtc gaaacggcgg cggacgctct gcgcatgtcg
      601 gctcacgcgg cgccctcgag gaaccgcacg atgcgttccg ccgtccagtc aggatgatca
      661 aggggaagcc gatgcccggc gccctcccgg acctcgcctt ctgtgccggc gctgtgcgct
      721 cgcagccagg ccgccgcgcc ggccgggatg atccggtcgc gtgtcccatg gagcagcaac
      781 cggggcatcc ttagccgtgc gcatgccggc cggaggtccg tccggaccag gtattccagt
      841 tgctcggcca gcgcctccgt ccccgattcg agcgctcggt gcgcgaactg cgccgccgtc
      901 tcgccgtcgg gctcggaagg agaggcggcg ttgatgaaaa agtcgcgaag ggttcgttcg
      961 ggcgtccgcc ggaaggacag ggtcatggcc tggagtatcc ggggcggaac cccttccggg
     1021 aaatcctggt cggccgccag ccgcggcgcc gccgagatca ggatgagcgc ggcgatccgt
     1081 ccgggggctg ccgccgcggc ctcgagggcc accatgccgc ccatggacca gccggccaga
     1141 atcacgggct cctcttgcgc ttctatctct tgcagcaggc ccgaggcata gcccgagagg
     1201 ccggatcgag gcgcggcgac atggattgcc tccagtccca gcaggcgaac cggccgctcc
     1261 gccaggcgat gcgcaagcgg ttcgagcgcg gcggccggat gcgcccatcc ggaaaccagc
     1321 agcaccggcg ccttcaagca acgttctccg cgcaggccgc cgcgatgatc gtttgggcgg
     1381 cctgcgccag gtccgcgggc tggtgggcga ggctgatcga gaaacggagc cgggccgtgc
     1441 cgggcggcac cgtgggcggg cgtatggcga cggccaggat gccgtcgtcc ttcaagcgcc
     1501 gtgagagggc gagggccttc gcgttgtcgc cgaccatcag cggcacgatc tggctggccg
     1561 aattcagcgt atccagcccg gcggctttca gcgtatcccg gaaaccggcg gcccgggcca
     1621 gcaattcctg tcccatttca ggcctctttg agagcaccat cagcgcgccc agcgccgcgc
     1681 ccaggacggc cggcggcggg gccgtggtat agatgaacgc gcgggccttg ttgaccagcc
     1741 acgcgcgaag gttctcggcg caggcgacga acccgccgta gctgccgagg gccttgctca
     1801 aggtacccat ggacacggcg acgcgatccg cgaggccgtg ttccgaaatc agcccggcgc
     1861 cgccgggccc gaatacgccg gtggcatggg cctcgtccac cagcatcagg gcctcgtgcc
     1921 gggccgccac ggcggccagg tcgggcaggg gggccaggtc gccgtccatg ctgaacacgc
     1981 tctccgtcag caccaggcgc cgccgggcgg aatgggcgga gagcagttcg tcgagatgca
     2041 acgcgtcgtt atgccggaag cggagcaggc gggcgcggct caagacggcg gcgtcgagga
     2101 ggctggcatg ggccaggcgg tcggccagca cgaggtcatc cggtccgacc agcgcgggaa
     2161 tcgtcccggc gttggccagg tagccgctcc cgaacacgag ggcggcgggg tagcccttga
     2221 gggcggcgag ccgctgttcg agttcctcgt ggaggggcag ggtgcccgtg atgagccggg
     2281 aagccgtggc gccggcgccg aattcatgca gggccttgac cgccgcttcc acgacgcgcg
     2341 gatggcaggc caggccgaga tagtcgttgc tcgacaggtt cagcagtgtg cgaccgccca
     2401 cggtgatccg cccgcccgtc gcgggaaacg ccgtcaaccg gcgctccagg tctcccgcgc
     2461 gcagttccgc cagcccgtcc tccagccagt tctcgccctg catggcggtc agtgtaagcg
     2521 actcatggac aggatgcgac ccttatgata agcctgcgcc accatgaact ggctggactg
     2581 cgggcagcgg gtgaaggcgg gcggcgcgtt gacgcgcgag gaagcgctgg ccgtgctgca
     2641 gtcatccgat gacgagatcc tggaagtggt gcaggccgct ttcctggcgc gccgccagca
     2701 cttcgggcgc gacgtgaacc tgcacatcct tcgcaacgcg cagagcgggc tctgcggcga
     2761 gaactgcgcc ttctgcagtc aatccgcgtc atccgcggcg gagatcgagc ggtatcctct
     2821 gcaatccatc gaggagatcg tggcgggcgc tctcgatgcg cagcgggccg gcgccgtgaa
     2881 gtattgcgtc gtcaccagcg gtcgcgcgcc ggcggcggat acgctggaca cattttgcga
     2941 ggcggcgcgg ctgatccggc gcgccacgtc cctgcatctg tgcgtttcgc ccgggctgct
     3001 cacggagggg caggcccgga ggctggccgg ggcgggggtc cacagggtca accataacct
     3061 ggaaaccgcg cgccgatatt tcccggccat ctgcaccacg cattcgtacg aggaccgcgt
     3121 ggccaccgtc cgggcggtca aggcggccgg catggaggct tgctgcggcg gcattctggg
     3181 gctgggcgaa acgctggagg acagggtgga cctggccttc gcgttgcgcg aactgggtgt
     3241 tgattccatc ccggtcaatt ttttcaatcc ccgccccggc acgccgctgg cggaccagcc
     3301 tccgctgggc gcgagggacg ggttgcgcgg gctggcgatg ttccggctgg tgaacccgac
     3361 gcgggacatc cgggcggcgg gggggcgcga ggcctgtctc ggggcgttgc agccgctggc
     3421 cctgtacgcc gccaattcca ttttcaccag cggctacctg accacgcctg gccagggccg
     3481 gcccgcggac atccggatgc tggaggaggc gggcttccgg ccggcgaagc tggagatgta
     3541 gcggcggctg ccgacatgga tgtccaggaa atcacgatcc gaagcgcgct ggtccgctcg
     3601 cggattcccg gtgtcgaata tgtcatcaat ccgtatctcg gatgcgggca tggttgccgt
     3661 tattgctacg ccgtgttcat gacgaagcac tcgcgtcacc atgccggtgc ggcatggggt
     3721 tcgtttgtgg aggcaaagac gaacatcgcc gctatattgc gggacgagtt gcgtcggaag
     3781 cggaagacgg cgacggcgat gctgtccagc gtatgtgatc cgtatcagcc ggcggagcgt
     3841 cggtacaagc tcacccgcgc gtgcctggaa gcgctccgcg acttcggctg gggcatggaa
     3901 atcctgaccc ggtcaccgtt ggtcctgcgc gacctcgata tcctgcagtc gggctcccgc
     3961 gtgtccgtgg gattttccgt cccgaccgat gacgaccgcg ttcgccgggt gaccgagccg
     4021 aacgcgcctc ccatcggcag tcgcatcgca accttgcgtc gtctgaagga ggcggggatt
     4081 cccacttggg ctttcatcgc gcccatgctg cccatgaacc cggagcggct ggctgaacta
     4141 ttggctcccg tgatcgattc cgtcatgatg tccgggttga actacgtcgg acgggtggcc
     4201 ggtctgtttc ggcggttggg ctgggcgtca gcgctgacga gggggtatgc gcgggaaatg
     4261 cgcgaacggc ttggccgatc attggacgcc aaaatgctcc atgccgcccc tccggaagag
     4321 ggctgactgt cggcgcggtt tccacacttt gcaaagtgtg gaattggatc gtatcatgtg
     4381 cgtggcacac aacgaaggag tatgatgact ggaccgattg taacgcctta tgtacaagaa
     4441 tataaagaat atagcgcgta tactcccgaa catgtccttc agtaactact tccacacttt
     4501 gcaaagtgtg gaaataggca atgacgtatc ggcagcggtt aacgagccgg gttagccaat
     4561 ggagatcatg cgctcaaccg cgcggagggc gcgcacgcga atgtcctccg ggacttcgac
     4621 cacgtagcgg ttgagcttca gggaattcaa ggtgtcctcc aacgtaatct cgttcatgtg
     4681 cgggcagcga atgctgcaga tacggaccat ctctttgtcc gggttttccg ccgcaatatt
     4741 gtcgcccatg ctgcactccg tcagcagaag atagcgctgg gccgcggatt ctttcacata
     4801 gcggaccatc gccatcgtgc tgcccgcgaa atccgaggcc cgcaccacct cgggccggca
     4861 ttccgggtgg gccaggacca ccacgtcggg aaattgacgg cgcgcatttt tcacatcctc
     4921 gaccgtgaag cggtcgtgca cctcgcatga accggtccag ccgatcatct ggtaatccag
     4981 attctccggc gcgccgccct tcctccggct ttttcgggca gggaagataa tgcgttttcc
     5041 ggtctccttc gcgacattgc gcgccagaaa ttcgtccggc aggaaaatga cacggtctgc
     5101 cttcagcgag ttcacgacgg ccgccgcgtt gccggacgtg cagcagatgt ccgattcggc
     5161 cttcacgtcg gcgtacgtgt tcacatacgt gacgaccggc acgcccggga attgctgctt
     5221 gagtttgcgc acgtcttccg cggtgatgct ctcggccagc gagcagccgg cgcgctgggc
     5281 cgggaggagc acggtccggc ggggattcag gatcttggcc gtctccgcca tgaacctgac
     5341 gccgcagaag acgatcacgt ccttgtccga ctgcgcggcc ttgcggctca actcgaggga
     5401 atccccggtg aaatcgggga cggaatggta gagcgccggc tccatgtagt tgtgcgccag
     5461 gatcacggcg ttgcgttcct gcttgagccg catgatctcg tcggccagcc cggccttgta
     5521 gcgcagttcc gcgtccggca cgacgtggcc caggcgcgcc ttcatctttt cgtacatttc
     5581 cgacgcggac atggatcacc tttcacagca aggtaggccg gttccgggat gaattcaacc
     5641 gcgcagtgcg agccggtttt ggttgcgccg cgggagcggc tgcacgatac tggagaaaag
     5701 caggttcgtc ttgagcaacg agaagcccag cgatcccgtt ccgccgtccc ctgaaatccc
     5761 ggagagcggc ccgcgccatc cccgcccggc gcgccgggaa caggccgcct gcgacgcgct
     5821 gcgcaccttc gtgcgcgatc tgcaccagga ccggttcgga accgcgccgc cccggctgga
     5881 ggagggggaa ctggtcctga agatgaaggc ccggcctggc gaggattggg cgcttcgttt
     5941 tgatcccccg ctaagcgagc aactggagac gcagctggat gattggcagg ccagccggga
     6001 catataccgg gagggacgcg tgtactgctt ccggtgcgac acttcggagt gcgaacatgc
     6061 ccggcccttc tcgcccctgg aggtgttcaa ggaatacgcg cccaacggca tcccggaatg
     6121 gcacgaactg gcccaggcgc tgctggcggg cggcgacgaa cgggcggagc ggctctaccg
     6181 ggagggcggc ggcctcgtgg cgctgttcca gcccggccgc ctgctgcgcg ggaagcaact
     6241 ctcttccttt gggcgatcat cccgcaccta tgccattctc gcgcaggtgg cctcgggctt
     6301 ctttccggcg ccggaaggat tatcgccgga gggggggcag gggagccgac tcgccatctc
     6361 gtttcaggcc gtcgaggtcc gcggtccgcg gggggaacga atcctccggc tcaatccggt
     6421 gacgcggggg gatgtggcgg gccttcgcga aagcctgact tcaggatggc agccggcgct
     6481 ctaccgggcc tggaagacgg ccgagcagga gatggaatac ctggagcgag cggcccgaga
     6541 ggccggagcg ggccgtgacg gggcgcgcgt ccacgaagtc atgaggaggc tgccgggtat
     6601 catgcgccgg ctggcggaat ccctggagcg gggaggccgc caggagagcc ggcggactca
     6661 tcacgtcgag cgccgccgcc acgaacaccg gcccgtgcac aaggccctcg aggacgcaca
     6721 ggcggccgcc cccggcctgg tgttcgacga cgagaaggcg ggcacgaccg tggcttgcgg
     6781 gccgcagtcc cgcgcgcatg ccttcaaccg ggatggtcgc cacgtgacca gttttgtgct
     6841 ccgaccgcag gcgatagacc tgcgcgtgag gacgcgccgg tggcgggtat tagccccgga
     6901 tgaggtggcg gaattcaagc ggcggattgc gcaatacgtg ccggctcaaa aagacatggc
     6961 gccgccgctc tcgcggtaga gctcgcccag gcgaaggctg gcggaacgct tgcttaaccc
     7021 cttctcgaaa atcagcacca tgtacaccgt gtcggccagc gcccggtgga gcacgcccgt
     7081 gtccaccttc gtataatggg ccacatcgga cagggtgtag gacttggccg acgggaacca
     7141 gcgccggaac aggggcaggc tgtcgaccac gaggttgttc aacaggggca attccgaccg
     7201 gcgaatctcc gcggccatga aggagacatc gaagcgggcg ttgtgcgcca tcagcacgct
     7261 gccctgcacg aagtcgacga actcgggata cacctgcttg aacgacggct tgtccgccac
     7321 gtcctcgtta gtgatgccgt gaacctcgat gacccacgga gggatccaac ggcccgggtt
     7381 gatcagccag cttttctctt caatgatctc gccgttcctg aattttaccg cgcctatttc
     7441 cacgatgcga tcgcccgccg ggctgaatcc cgtggtctcg gtgtcaaagg ctacaaatgt
     7501 tacattgtcc accaacgtat tgcggtgggg catcttctcg aactcgtaat taggcgggac
     7561 gcgcttggaa tcggctgccg gggcgtcaag gaaaagaaga gtgcccgaag tcaggatcgc
     7621 ggcgagagac gtgagcatga gcgcacgcct tcgacgttgg acgaccaatc tgttttttaa
     7681 taagtacagg attcagcctt ggttatggtc gaacgcatgc ctcttatttg catttataat
     7741 accagtaata acggaaacgt caatagctgt ttgcgcaata taacttgaca agttatcaac
     7801 atgtcgagtt tgatattaaa tatgaccggg ccatttcaag aacgagaacg ccgcggaaca
     7861 tattcatttc tgttttggtt gtcagaactc ccgcaacgct tataatccgc gcggtgtttt
     7921 tgccggagaa tggtccgcga accggccgga cggggacgga atccgcgatg gcattgcgat
     7981 tttacaatac gttgacgaga cagttggaca cgtttgagcc cctcgaggaa ggccgcgtgc
     8041 gcctgtacac gtgcggcccg acggtttacc actatgcgca catcggcaat ttccgctcgt
     8101 acatgttcga agacctgttg cgtcgatact tgcggttcaa gggcttccgc gtcacccagg
     8161 tgatgaacct taccgacgtg gacgataaga cgatcagggg cgcccgcgaa cgaggcgtct
     8221 cgctcaacga gtatacgcag cagtacaagg acacgttctt cgaagacctg aaaacgctga
     8281 acatcgagcc ggccgaacac tatcccgcgg ccaccgatca cgtgcccgag atgatcgccc
     8341 tgatcgaacg gctcatggaa aaaggagtcg cttaccgctc ggacgacggc tcggtctact
     8401 tcagcatcgc gaagtttccc gcctacggcc gactggccca cctcgatctc tccgggctcc
     8461 gggcgggcgc ccgcgtggcc caggacgaat acgagaagga caacgtcgcc gacttcgcgc
     8521 tctggaaggc ctgggacgag aacgatggcg aggtggcgtg ggattcgccg tggggccgcg
     8581 ggcgcccggg ctggcacatc gagtgctcgg ccatgagcat gaaatatctc ggcgagagct
     8641 tcgacatcca taccggcggc gtggacaaca tgttcccgca ccacgaggac gagatcgcgc
     8701 agagcgaggc cgcgacgggc aagccgttcg tgaagtactg gctccactgc gcgcacctga
     8761 tcgtcgacgg ccgcaagatg tccaagtcgc tgggcaactt tttcacgctg cgcgacgtga
     8821 tcggccgggg gttttccggg cgcgaggtcc gctatgtgct catggccacg cactaccggc
     8881 agtggctgaa tttcacgttc gacacgctcc gggcggcccg cgcggcgctg gaccgcctcg
     8941 acgagttccg ggcgcgtctg gaaaagacgt cggcgggcgc cgccgccggg ccggcgccgg
     9001 cctgggcgga tgaggcgctg gaccggttcg cggccgcgat ggacgaggat ctgaacatct
     9061 ccggcgccct cggcgcgctg ttcgacatga tccacgccgg gaacaaggcg ctggacggcg
     9121 gcacggtgga tgcgccgacc gccggagggg cgctcgccac gctgcgcgcg ctggaccggg
     9181 tgctcggact gctgcaggag acgggcgaga cggccgacgc ggccgcccgc gagctggccg
     9241 cgctccggct gaaggcccgg gaggccaaga actggccgga ggccgaccgg ctccgcaaag
     9301 aactgctcgc cctcggctgg ctggtcaagg acactccgca ggggccggaa ttgaaaaagg
     9361 cgtgaacgtc cgacaaactt ctaattcacc tgtttcatgc gaggcaaatt catcacgttc
     9421 gaggggcccg agggcggcgg caagaccacc caggcgcgcc gcctgatcgc ccgcctggaa
     9481 aaggacggcc acccggtact gtacgcccgc gagccgggcg gcacgccgac gggcgaagcg
     9541 atccgcgata tcctgcaata cgacaaggcc ggggagccga tctgtcgcga gaccgaggtg
     9601 ctcctcttcg ccgccagccg cgcccagctc gtccgccacg tcatcctgcc cgcgctcgag
     9661 gccgggcgcc aggtggtctg cgaccgcttc gcggattcga cgaccgccta ccaaggcttc
     9721 ggccgcggct tcccggtcga gcagatgctc acaatcaacg agttcgcgat caacggcgcg
     9781 catcccgacc tgacgctcct gctggacatc gacgtgggca tgggcttcga gcggctcacc
     9841 cgtcggcacc gggagacggc cagcacctgg gaccggatcg agcgcgagga gcgcgccttc
     9901 cacgagaagg tccgccaggg ttacctggaa ctcgcccggc gctggccagc gcggttccgc
     9961 atcgtgaacg ccggccggga tcccgacgcc gtggaacagg agatctggga ccatgtccgc
    10021 cggctcctcg cttgaccgct ttgccgagct gttcgccagc ctgcgccagg ggcatgccgc
    10081 cggccggctg gcccatgcct acctggtggt cggcgcgccc cgcggcaacg ccctggccct
    10141 gtcggaggcg ctgctccagc tgctcttctg cgcgggcgag ccgaggccgt gcggcgagtg
    10201 cgccgaatgc cggcgggtgc gcgaacgcgc ccatcccgac atcctctgga tcgagccgga
    10261 gagcaagagc cgccggatca agatcgagcg gatccgcgag gaactgatcc cgcggctgtc
    10321 ccagggctcg tacggcggcg gctggaaagc cggcgtactg atccaggccg accgcctgac
    10381 gggagaggcg gccaacgcct tcctcaagat gctcgaggag ccgccgccct ccagcctgat
    10441 cctgctcctg accgactcgc cccaggaact gctgccgacc atcgtttcgc gctgccatcg
    10501 tctcctgctc tccgcggaac cggcggcgcg gacggagccc tggtatgcct cgcttctcga
    10561 tatcctgcgg tctgacgcgc cgtcggaccc gctgcaggcc ctcggccggg tcgcccggat
    10621 gaaagcgctc ctcgagagcg tgcgcgcggc agccgaaagc gaggcgcctc ccgcggcggc
    10681 cggcgaggcg gagccggagg aggacgtcga ggacgcccgc gtccgcacgc g
//
LOCUS       DAOOTK010000109        10430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4159_length_10430_cov_4.860434, whole genome shotgun sequence.
ACCESSION   DAOOTK010000109 DAOOTK010000000
VERSION     DAOOTK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_10430_cov_4.860434"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /gene="pbpC"
                     /locus_tag="PK634_11185"
     CDS             complement(<1..1669)
                     /gene="pbpC"
                     /locus_tag="PK634_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOW98339.1"
                     /translation="MMGYARWLLRGAAGALAGGGIVAVLNACYPFPRAALDKFPAATV
                     LADREGNPLRLKLGPGDMDNVPLYRWREDDWIAKAVVAAEDQRFYRHVGIDPLALLRA
                     VGQNVLHLRRVSGASTLSTQVIRLVQPRPRTLRTKVIETFRALQLERALTKREILEQY
                     LNRAPFGSNIVGIESAARRYFSKSAHDLNLAEAALLAGLPQAPARFRPDRHPARARKR
                     QAYVLERMAALEMITEDQRARALERPCDIKPGRHAFTAPHFAQFVLDRLPPDRDGTTL
                     RTTLDPSLQSLAETSLARRHGYLDPLGIMGGAVVILEVKTGAVRAMVGSPDYFDAARR
                     GQVNGALAPRGAGSTLKPFVYAAAFDRGLATPLTVLRDEPALYRDYRPENFGRTFRGD
                     ITVRDSLILSLNLPVLALAERIGAERLHALFRRAGLKTLGRGTEHYGLGLVLGQGEVR
                     LLDLANAYAAFARGGVWRSWTVWEGQAPGAPRRLFSPDASWLVADILGGDERMEELSG
                     HLADARMPRMAWKTGTSSACRDAWTVAFNPEYVVGVWIGNPGGEADEG"
     gene            complement(1690..7383)
                     /locus_tag="PK634_11190"
     CDS             complement(1690..7383)
                     /locus_tag="PK634_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOW98340.1"
                     /translation="MKLEPELSRRVEEYLSQVEENLRARPPAVRREIMDSLRRHIHEA
                     LAARSGSPSTEEVDLVLADMDPPESFAAEGETSVTASAMAPPVPLLRWHWFLLACTFL
                     ALNGWAVWKWSRAPAQGDGRVLRVQFSARPDQPLQGQEPLSWAFNEDVVGSEAVGAPK
                     PEGPVSLRPAVPGRYLWKSPRVLVFTPDGTWPLCSEFESLLDAEFFGSLGMTFRNPGP
                     KRFRTPALNLASVAQVDFTPDRRATLRLQFNAPTDTRLLGKFLSLATSSQSNLSYEIS
                     APAPSSAVVLVQTEMVEQDAIEVTLEPGVPASTGPLGLEKRIVRQVPLSSSFRLTGVE
                     AASPSFEQPSVTLSFSTAPDAQGAREFIEVQPAASFSVEPLNSWWSSGLRLTGGFEPG
                     RVYTVTLKPGLRSESGLSLAQPITRRVNFPSRDPALCFSAEGRYLSPSGSLRIPLMHV
                     NARECRLAAYPVPPQNLIQLALREAGRMEHFWAWGGDGLVEKLTAPAWVYPTNALDSR
                     PETVARTAVDLARLTEGPPRGAYWLTAEPEKGQGTRQLVMITDLGLSVKSADDGMLVW
                     VNSLSAAQPVADVEVKLYAENNVELASGTTDGDGLSFLALDEKGWEQSPLLVVASLSN
                     DVSCLNLDQGGVSLPAGEGDRAFLAEGYEAYVFTARGVFRPGETIETKAVVRDRALKC
                     PEPFPVLFRIVRPDGRVFRDVPAMLDPLGAAEMKIEMPDYLPTGQYTVELDLPGTFKT
                     LGSTVIAFEDFFPPQVRVDVKPPADPLQPGVESDFTVHADHLFGKPAAGLPVSGFVVW
                     KTLVFEPKGWADFIFGDGEKEFTAIYSSAGQGRLDEAGGFEFPLEPSAGWRPPARLQA
                     VISGTVMESGGRAVTASAACPFDPYPFYIGLKNAATRMAAVGAAQSLELALVRPDEQL
                     LATNMALRVNLYRSVWNTVLKRNAQNRFTYQSEQQLIPAGEELVQLQDGRVLHSFKVD
                     QAGSYLLRVTDPASGASSSLRFYATEPGGTGVAWGGDRPDKVALTADRDAYAPGESAR
                     VTIQAPFAGTALLTVENDRILDRRVVRMTNSSELVEIPLRDEYAPGVYAAVTMIRPAV
                     AESVWTAHRAAGIAYLPVKPAGRRLTVAVEAPEILPPQQAAKIKVRVAGEQGPVADAD
                     VTLMAVDEGICMLTDFQTPDPLAWFLAPRWFATSLYDLYSLLMPLLDDEATAAVSPPG
                     DGEEGLGKRLNPIRTRRFKPLALWKSGIRTDASGEAEVMLDIPEFAGRLRLMAVAFTR
                     DQAGSAERAALVKRSLVVQTSLPRFLAPGDEAEMTVSLFNETDDQRAARIRVTCGGPL
                     SVEKSEWTQDLAAQASAVLRIPLRAGAAPGAALCQVEVASGTDVYRESVELSVRPVSP
                     REIRFLHGEMKAGAQFTIEAPDDWIAESVWGELLCLSGPEGRWKGALDWLTAYPYGCI
                     EQVTSTVFPLLYLDDMRKTLWPDLAGPEADRETILSGVYRVLSMQLGNGGFSLWPGGT
                     QVYPWGSLYATHFLIEARKAGIPVPEDRLDSALAWVRGRLDARAPADASPDSDDWQTD
                     MEERTYACLLLALAGTPSDSWMSRLEEQASLLRPGTRINLALALMHGGEPGLAGGVLE
                     RLGIPPPGMPRARGRTLNSDVRDAALLLSAWLERDPKNPLAPQLARWLDDAQQDGRWL
                     TTQDNAFALMALGKYARTRPRDPKPWTAEWAANGAPPVVSGPTNSFRRANGNVPWPAS
                     GILANRGPGEFFYTVRLEGVPAADRPAAERDDGLQIRREWLDLDGNPVDPESVPQGQL
                     VVVKLTLDTLGEPMDNLVIEDLLPAGFDVENPALATAQVVPWIRDRETGVAGRQARDD
                     RMLFFTGSISGRHAFYYSMRAVTPGEYVVPPSVVECMYDPALRSVNGGGRAIVLE"
     gene            complement(7367..7723)
                     /locus_tag="PK634_11195"
     CDS             complement(7367..7723)
                     /locus_tag="PK634_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOW98341.1"
                     /translation="MDGWITQLRKGLLDYCVLLLLRRGESYGYEIVQELKRIEELAVS
                     ESTVYPILGRLRSEKLVKTRDVPSPEGPARRYFSLTALGRLRLAEMNAYWKSLDRALG
                     RLREDAKEDRPDEAGA"
     gene            complement(7817..8692)
                     /locus_tag="PK634_11200"
     CDS             complement(7817..8692)
                     /locus_tag="PK634_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOW98342.1"
                     /translation="MTNWSKIVLLGHTGFIGSRLADGFRKAYPGAPIEGRSFPAFDLA
                     GPDAHAELASLFDENALVVFLSCIKRQFGDDLDTFDRNVQMARSVCRALKARPVTRLI
                     FFSSAAVYGEDIHNTAITEDAPICPTSFYGMAKFVSERMLWKTMTDAGRGSLAIIRPP
                     TVYGPGDAGKTYGPSGFIQAAVKGEPITLWGDGEELRDFLWLDDLVEAVIALAANSFA
                     GVLNLVTGRSVTFRQMLDIISARLGRPLDIKSRPRSKSKVDNVFVNDRLRTVLPGLRF
                     TPMEEGIARLLSAAG"
     gene            complement(8689..9723)
                     /locus_tag="PK634_11205"
     CDS             complement(8689..9723)
                     /locus_tag="PK634_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOW98343.1"
                     /translation="MKALAAILVESKKPLVLEEIEIPPLRFGQVLVKVICTGICGAQI
                     NEIDAVKGPDKFLPHLLGHEATATVVEVGEGVTTVKPGDRVVMHWRKGSGIHAPTPKY
                     KSPVGTVNSGWVTTFNEMAIVSENRVTTVPSDFDPEAGALMGCAVTTAFGVINNDAGV
                     RVGESVAVFGAGGVGLNVVQAAAMVSAHPIIAIDLFDSKLELAGRLGATHLINSKKQD
                     AAAEILKIVGKNGADVVVEVTGNARVIETAYEVTQAQGRTILVGVPREKATIYTLPLH
                     HDKILRGSEGGQTRPERDIPNYVKLCQAGKLNLKQLITNHYPFEKINDAIADIRAGNV
                     PGRCIVQMKA"
     gene            complement(9817..10422)
                     /locus_tag="PK634_11210"
     CDS             complement(9817..10422)
                     /locus_tag="PK634_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW98344.1"
                     /translation="MCPDTRRPRECAAIAKKWGQEYWDGPRQYGFGGYKYDGRWLPFA
                     QKLAKHYGIKPGHRILDVGCGKGFLLHEFTRAVPGVEVAGLDISQYGIDNAKEEVKPF
                     LTVGHCRKLPYPDKHFDFVYSNTTLHNLQPQDLYPAIKEIERVRKGEAWIGVESYRNE
                     KEKANLLYWQLTCEAFFSPDGWRWWFDHCGYQGDYGFIYFE"
BASE COUNT         1718 a         3628 c         3431 g         1653 t
ORIGIN      
        1 ggccctcatc ggcctcgccg cccggattcc cgatccagac cccgacgacg tactccgggt
       61 tgaaggcgac ggtccacgcg tcgcggcagg cggaactcgt gcccgtcttc caggccatcc
      121 tcggcatccg ggcgtcggct agatgcccgg acagttcttc catccgttcg tcgccgccga
      181 gaatatccgc aacgagccag gaggcatcag ggctgaacag gcggcgcggc gcgcctggcg
      241 cttgtccctc ccacaccgtc caggagcgcc acacgccgcc ccgcgcgaac gcggcgtagg
      301 cgttggccag gtcgagcagg cgcacctcgc cctggcccag gacgaggccc aggccgtaat
      361 gttccgtgcc gcgcccgagc gttttgaggc cggcccgccg gaacagggca tgcaaccgtt
      421 ccgcgccaat ccgttccgcc agcgccagca cgggcaggtt gagagacagg atcagggagt
      481 cgcgcaccgt gatgtcgccc cggaacgtcc ttccgaaatt ttccggccgg tagtcccggt
      541 acagggccgg ctcgtcccgc agcaccgtca gcggcgtcgc cagcccgcga tcgaacgccg
      601 cggcgtacac gaacggcttg agcgtggacc ccgccccgcg gggcgcgagc gcgccgttga
      661 cctggccgcg gcgcgccgca tcgaaataat cgggcgaccc gaccatcgcc cggacggccc
      721 cggtcttcac ctccaggatc accaccgccc cgcccatgat acctagaggg tctaggtatc
      781 catgccggcg ggccagcgaa gtttccgcca gggactgcag ggacggatcc agcgtggtgc
      841 gcagcgtcgt gccgtcccgg tccggcggca ggcggtccag gacgaactgg gcaaaatgcg
      901 gcgcggtgaa cgcgtgccgg cccggcttga tatcgcaggg acgctcgagc gcccgggcgc
      961 gctggtcctc cgtgatcatc tccagggcgg ccatgcgctc cagcacgtag gcctgccgtt
     1021 tcctcgcgcg cgcggggtgc cggtccggcc ggaagcgcgc gggggcttgc ggcaggccgg
     1081 cgaggagcgc cgcctcggcc aggttcaagt cgtgcgcgga cttgctgaag tagcgccgcg
     1141 cggccgattc gatcccgaca atgtttgatc cgaacggcgc gcggttgagg tactgctcga
     1201 gaatttcccg cttcgtcagc gcgcgttcta actgcagagc gcggaaggtc tcgatcactt
     1261 tcgtgcgcag cgtgcgcggg cgcggttgca cgagacggat cacctgcgtg gagagcgtcg
     1321 acgccccgga cacacgccgg agatgcagga cgttctgccc gaccgcccgg agcagggcca
     1381 gcggatcgat gccgacgtgc cggtagaagc gctggtcctc cgccgcgaca accgccttcg
     1441 caatccagtc gtcctcccgc cacctgtaca gcgggacatt atccatgtcg cccggcccca
     1501 gtttcaggcg caggggattt ccctcccggt ccgccaggac ggtggccgcg ggaaacttgt
     1561 cgagcgcagc ccgcggaaac ggataacagg cgttaaggac ggcaacgatt cccccgccgg
     1621 ccagcgctcc ggccgccccc cggagaagcc atcgggcata acccatcatt ccgttagtcg
     1681 ggcacttcct cattccagca ctatcgctcg cccgcccccg ttcacgctgc ggagggccgg
     1741 gtcgtacatg cattccacca ccgacggcgg gacgacgtac tcgccgggtg tgacggcccg
     1801 catggaatag tagaaggcat ggcggccgct gattgaaccg gtgaagaaca gcatccggtc
     1861 atcccgggcc tgccggccgg ccacgccggt ctcccggtcc cggatccacg gcacgacctg
     1921 cgcggtcgcc agggcgggat tctcaacatc aaatccggcc ggcagcaggt cctcgatcac
     1981 caggttgtcc atcggctcgc ccagggtgtc gagggtcagc ttgacgacga ccagttggcc
     2041 ttgcgggacg gactccgggt ccacgggatt gccgtcgagg tccagccatt cccgccgtat
     2101 ctgcaacccg tcgtcgcgct ccgccgctgg acggtcagcc gccggcacgc cttccagccg
     2161 cacggtgtag aagaactcgc ccggcccgcg gttggccagt attccggacg ccggccacgg
     2221 cacgttcccg ttggcgcgcc ggaaggagtt ggtcggaccg gagacgacag gaggagcgcc
     2281 gttcgcggcc cactcggctg tccagggctt gggatcgcgc ggccgggtcc gggcgtactt
     2341 cccgagcgcc atgagcgcga aggcgttatc ctgggtcgtc agccagcggc cgtcctgctg
     2401 ggcgtcgtcc agccagcggg ccagctgggg cgccagcggg ttcttcggat cgcgctccag
     2461 ccaggcggac agcagcaggg ccgcgtcccg cacatcgctg ttgagcgtgc ggccgcgggc
     2521 tctcggcatg cccggcggcg ggatgcccag ccgctccaac acgccgcccg cgaggccggg
     2581 ctcgccgcca tgcatcagtg ccaatgcgag attgatccgc gtgcccggcc gcagcaacga
     2641 tgcttgctcc tcgaggcggg acatccaact atccgacggt gtgcccgcaa gggccaggag
     2701 caggcaggcg taggtgcgct cctccatgtc ggtctgccaa tcgtcgctgt cgggcgacgc
     2761 gtcggccggc gcccgcgcgt ccagccggcc acggacccag gccagcgcgg aatccagccg
     2821 gtcctccggc accgggatcc ccgccttgcg cgcctcgatc aggaaatgcg tggcgtacag
     2881 actgccccac gggtagacct gcgtcccgcc gggccacaag ctgaacccgc cgttaccgag
     2941 ttgcatcgag agcacgcggt agacgcccga cagaatcgtc tcgcggtcgg cctccggccc
     3001 ggcgaggtcg ggccaaaggg tcttccgcat gtcgtcgagg tacaacagcg ggaacacggt
     3061 cgaggtaacc tgctcaatgc atccgtacgg atacgccgtc agccagtcca gcgccccctt
     3121 ccagcggccc tccggaccgg acaggcagag cagttcgccc cagaccgatt ccgcgatcca
     3181 gtcgtccggc gcctcgatcg tgaattgcgc gccggccttc atttcgccgt gcaggaaccg
     3241 gatttccctc ggcgagaccg gccgcacgga caactcgacg ctctcgcggt agacgtccgt
     3301 cccggacgcg acttccacct ggcacagcgc tgcgcccggg gccgcgcccg cgcgcagcgg
     3361 gatgcgcagc accgccgagg cctgcgccgc gaggtcctgc gtccactccg acttctccac
     3421 ggaaagcggc cccccgcagg tcacgcggat ccgcgccgcc cgttggtcat cggtttcgtt
     3481 gaacaaagaa accgtcatct cggcctcatc gccgggcgcg agaaaccgcg gtaaactggt
     3541 ctggacgacc agcgagcgct tgaccagcgc cgcgcgttcg gccgacccgg cctggtcgcg
     3601 ggtaaaggcg acggccatga gccggagccg cccggcgaac tcggggatgt ccagcatcac
     3661 ctccgcttca cccgaggcat cggtcctgat cccggacttc cacagggcca gcggcttgaa
     3721 acgccgggtt cggatgggat tcaagcgttt acccaaaccc tcctccccat cgcccggcgg
     3781 gctgacggcg gcggtcgcct cgtcgtcgag cagcggcatg agcaggctgt acaggtcata
     3841 gagcgacgtc gcgaaccagc gcggggccag gaaccaggcc agcgggtccg gcgtctggaa
     3901 gtcggtcagc atgcagatgc cctcgtccac ggccatcagc gtcacgtcgg cgtccgccac
     3961 gggtccctgc tcgccggcga cccggacctt gatcttcgcg gcctgctgcg gcggcaggat
     4021 ctcgggggcc tcgacggcca cggtcagccg gcggccggcc ggcttgacgg gcaggtacgc
     4081 gatcccggcg gcacggtgtg cggtccagac cgactcggcc acggccggac ggatcatcgt
     4141 caccgccgcg tacacgcccg gcgcgtactc gtcgcggagc gggatctcga ccagctccga
     4201 cgaattggtc atccgcacca cgcgccggtc caggatacgg tcgttctcca cggtcagcag
     4261 cgccgttccg gcgaacgggg cctggatcgt cacgcgcgcg gactcgccgg gcgcataggc
     4321 gtcgcggtcc gcggtcaacg cgaccttgtc cggccggtcg ccgccccagg ccacgccggt
     4381 cccgccgggc tccgtggcat agaagcgcag gctcgacgag gccccgctgg ccgggtccgt
     4441 gacccgcaag agatacgatc cggcttgatc caccttgaac gagtgcagca cgcgcccgtc
     4501 ttggagttgg accagttcct cgcccgccgg aataagctgt tgctccgact ggtacgtgaa
     4561 gcggttctgc gcgttgcgct tgagcaccgt gttccacacc gagcggtaca ggttcacgcg
     4621 gagagccatg ttggtggcca gcagctgctc gtccggccgg accagagcca gttcgaggga
     4681 ttgcgcggcg ccgactgccg ccatgcgcgt cgcggcgttc ttgaggccga tatagaacgg
     4741 atacggatca aacggacagg ccgccgaggc ggtcacggcc cgcccgcccg attccatcac
     4801 ggttccgctg atcaccgcct gcaggcgcgc gggcggacgc caccccgccg acggctcgag
     4861 cgggaactcg aatccgcccg cctcgtccag ccggccctga ccggcggacg agtagatggc
     4921 cgtgaactct ttctctccgt cgccaaagat gaaatccgcc cagcctttcg gctcgaaaac
     4981 cagagttttc caaaccacga acccgctgac aggcagaccg gccgccggct tgccgaacag
     5041 gtggtcggca tgaaccgtga aatcgctctc cacgccgggc tgcagcggat cggccggagg
     5101 cttcacatcc acgcggacct gcggcgggaa gaaatcttca aaggcgatga cagtgctgcc
     5161 cagcgtcttg aatgtgccgg gcaggtcgag ttccacggta tactggccgg tgggcaggta
     5221 atccggcatc tcgatcttca tttccgccgc gcccaaaggg tcgagcatcg cgggcacgtc
     5281 acggaagacg cggccgtcgg gccgcacgat acgaaacagg acggggaacg gttccgggca
     5341 tttcagggca cggtcgcgca cgaccgcctt ggtctcgatc gtctcgcccg gccggaacac
     5401 gcctcgcgcc gtgaaaacat aggcctcgta cccttcggcg aggaacgccc ggtccccctc
     5461 gcccgcgggc agcgacacgc cgccctgatc gaggttgagg caggacacat cgttcgacag
     5521 cgaggcgacg acgagcagcg gcgactgctc ccagcccttt tcatcgagcg ccaggaagga
     5581 aaggccatcg ccgtccgtgg tcccgctcgc cagctccacg ttgttctcgg catagagctt
     5641 cacctcgacg tccgctacgg gttgcgccgc ggacagcgag ttcacccata ccagcatgcc
     5701 gtcgtccgcg gacttcacgg agaggcccag gtcggtgatc atgaccagct ggcgagttcc
     5761 ctgccccttc tccggctcgg ccgtcagcca gtacgcgccg cgcggcggac cttcggtaag
     5821 ccgcgccagg tccacggccg ttcgcgcaac ggtctcgggc cgcgaatcga gcgcgttcgt
     5881 cgggtacacc caggccggcg cggtgagctt ttccaccagc ccgtcgccgc cccaggccca
     5941 gaaatgctcc atgcggccgg cctcgcgcag ggccagctgg atgaggttct gcggcgggac
     6001 gggataggcg gcgagccggc actctcgcgc gttgacgtgc atcaggggga tgcgcaggga
     6061 gccagacggc gacaggtatc gtccctcggc ggagaagcag agcgccggat cgcgcgaggg
     6121 gaaattgacc cggcgcgtga tgggctgcgc cagcgacagc ccgctctccg accgcagacc
     6181 gggcttcagc gtgacggtgt agacgcggcc cggctcgaac ccgccggtca gccgcaagcc
     6241 gctggaccac cagctattga gcggctctac cgagaacgag gccgccggct gcacctcgat
     6301 gaactcccgc gcgccctgcg cgtcgggcgc cgtgctgaag gagagggtca ctgacggctg
     6361 ttcgaaagat gggctggccg cctcgacgcc ggtcaggcga aaggaggagg acagcggcac
     6421 ctggcgcacg atccgcttct ccaaacccag cgggccagtc gaggccggaa cgcccggctc
     6481 cagcgtcact tcgatggcgt cctgttccac catttccgtc tgcacgagca ccacggccga
     6541 cgacggggcc ggcgctgaaa tctcgtacga cagattggat tgcgaactcg tggcgagcga
     6601 aaggaatttg ccgagcagcc gcgtatccgt cggcgcgttg aactggagcc ggagcgtggc
     6661 ccggcggtcg ggagtgaaat ccacctgcgc gaccgaggcc aggttcaggg caggcgtgcg
     6721 gaagcgtttc gggccgggat tccgaaacgt catgcccagc gagccgaaga actccgcgtc
     6781 cagcaaagac tcgaattccg agcacagcgg ccaggtgcca tccggcgtga agacgagcac
     6841 gcgcggcgat ttccagagat agcgccccgg aaccgccggg cgcagcgaaa cgggaccttc
     6901 gggtttcgga gcccccaccg cttccgaccc gaccacgtcc tcgttgaacg cccacgagag
     6961 gggttcctgc ccttgcagcg gctggtcggg tcgcgccgag aactgcacgc gcaggacacg
     7021 gccgtcccct tgcgccggcg cgcgcgacca cttccacaca gcccagccgt tcagcgcgag
     7081 aaaggtgcag gccagcagga accagtgcca gcgcaggagc gggaccggcg gcgccatggc
     7141 cgaggcggtc accgacgtct ctccttcggc ggcgaaggat tcgggcgggt ccatgtcggc
     7201 cagcacaaga tccacctcct ccgtggacgg gcttcccgac ctggcggcaa gggcctcgtg
     7261 aatatggcgg cgcagcgagt ccatgatttc tcggcgcacg gccggcgggc gcgcgcgcag
     7321 gttttcctcc acttgggaca agtactcctc tacgcggcgg gacagttcag gctccagctt
     7381 catccgggcg atcctccttg gcgtcctcgc gcaagcggcc gagggcgcga tccagcgatt
     7441 tccagtaagc gttcatctcg gccaagcgca gccggccgag ggcggtcagt gaaaaatatc
     7501 tccgggcggg cccctccggc gaagggacgt cgcgggtctt cacaagtttc tccgaacgca
     7561 accgcccaag gatcggatag accgtgctct ccgaaacggc gagctcctct atgcgcttga
     7621 gctcctgcac gatctcgtag ccatagctct ccccgcggcg cagcagcaaa agcacgcagt
     7681 agtccagcag ccccttccgc aattgtgtga tccacccgtc cacggctact ccgtattaag
     7741 tattatttaa tacatagtag cgtgcccgcg catcgatgca agggaaatat gcgtgagatt
     7801 tcttcgcgcc cttcgttcaa cccgctgccg acagcagacg cgcaatcccc tcctccatcg
     7861 gggtgaaccg caggcccggc aggacggtcc ggaggcgatc gttgacgaag acgttgtcca
     7921 ccttgctctt gctgcgcggg cgcgacttga tgtccagcgg cctcccgagt cgggcggaaa
     7981 tgatatcgag catctgccgg aaggtgacgc ttcggcccgt gacgagattc agcacgccgg
     8041 caaacgaatt cgcggccagc gcgatcaccg cctcgacgag gtcgtcgagc cacaggaaat
     8101 cgcgcagttc ctcgccgtcg ccccacaatg tgatcggttc gcccttcacc gcggcctgga
     8161 tgaagccgga aggcccgtag gtcttccccg cgtcgcccgg cccgtacacg gtcgggggcc
     8221 ggatgatcgc cagcgagccc cgcccggcat cggtcatggt cttccagagc atccgttcgg
     8281 atacgaactt ggccatgccg tagaaggacg tcgggcagat cggcgcgtcc tcggtaatcg
     8341 ccgtgttgtg gatgtcctcg ccgtacaccg cggccgagct gaagaagatg agccgcgtta
     8401 ccggccgggc cttcagggcg cggcagacgg agcgcgccat ctgtacgttc ctgtcgaacg
     8461 tgtccaggtc gtcgccgaac tggcgcttga tgcaggagag gaatacgacc agcgcgtttt
     8521 catcaaagag cgacgccagt tccgcgtgag cgtcgggccc ggcgagatcg aacgcgggga
     8581 acgaccgtcc ttcgatgggc gcgccgggat aggccttgcg aaaaccatcc gccagccggg
     8641 acccgatgaa ccccgtgtga ccgaggagga cgattttcga ccagttcgtc atgccttcat
     8701 ctgcacgatg caccggccgg gcacgttgcc ggcgcggatg tcggcgatgg cgtcgttgat
     8761 cttctcgaac ggataatggt tcgtgatgag ttgcttcagg ttcagcttgc cggcctggca
     8821 gagcttgacg tagttcggga tgtcccgctc gggccgcgtc tggccgccct cggagccgcg
     8881 caggatcttg tcgtggtgga gcggcagcgt gtagatcgtc gccttctcgc gcggcacgcc
     8941 gacgaggatc gtccggccct gcgcctgcgt cacctcgtac gcggtctcga tcacccgcgc
     9001 gttgccggtg acctcgacca cgacgtccgc gccgttcttg ccgacgatct tcagaatctc
     9061 ggccgccgcg tcctgcttct tcgagttgat caggtgcgtc gcgcccagcc tgccggccag
     9121 ttcaagtttc gagtcgaaca gatcgatcgc gatgatcggg tgcgcggaga ccatggcggc
     9181 cgcctggacc acgttcagcc cgacgccgcc cgccccgaac acggccaccg attcgcccac
     9241 gcggacaccg gcgtcgttgt tgatcacgcc gaacgccgtc gtcacggcgc agcccatcag
     9301 cgcgccggcc tcgggatcga agtcggacgg caccgtcgtg acgcggttct cggagacgat
     9361 ggccatctcg ttgaacgtcg tcacccagcc cgagttgacc gtgccgacgg gcgacttgta
     9421 cttcggcgtc ggggcatgga tgccggagcc cttacgccag tgcatgacca cgcggtcgcc
     9481 gggcttgacc gtcgtcacgc cctcgcccac ctccacgacg gtggccgtcg cctcatggcc
     9541 gagcaggtgg ggcaggaatt tgtccggccc cttgacggcg tcgatctcgt tgatctgcgc
     9601 gccgcagatc cccgtgcaga tgaccttgac cagcacctgc ccgaaccgca gcggcgggat
     9661 ttcgatctcc tccaggacca ggggcttctt gctttcgacg aggatggcgg cgagagcttt
     9721 catgcttttt ccttcatgtc gtgattcagg tagggcggct tcgctgaagc cgccgcactt
     9781 cggcgcgttg agccaacgcg ccctaccagg acctgttcac tcaaagtaga taaacccgta
     9841 atcgccctgg tacccgcagt ggtcgaacca ccagcgccag ccgtcggggg agaagaacgc
     9901 ctcgcaggtc agctgccagt acagcaggtt ggccttctcc ttctcgttgc ggtaggactc
     9961 gacgccgatc caggcctcgc ccttgcgcac gcgctcgatt tccttgatcg cgggatagag
    10021 gtcctgcggc tggaggttgt gcagggtcgt gttggagtag acaaagtcga agtgcttgtc
    10081 gggatacggc agcttacggc agtgcccgac ggtcaggaac ggcttgacct cttccttggc
    10141 gttatcgatg ccgtactgcg agatatcgag gcccgcgacc tcgacgcccg gcaccgcgcg
    10201 cgtgaactcg tgcaggagga agcccttgcc gcagccgacg tccaggatcc ggtggcccgg
    10261 cttgatgccg tagtgtttag ccagcttctg cgcaaacggg agccagcggc cgtcatactt
    10321 gtacccgccg aagccatact gccgcgggcc gtcccagtat tcctggcccc acttcttcgc
    10381 gatggccgcg cactcgcgtg gcctgcgcgt gtcggggcac ataccggcat
//
LOCUS       DAOOTK010000110        10218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4271_length_10218_cov_4.198662, whole genome shotgun sequence.
ACCESSION   DAOOTK010000110 DAOOTK010000000
VERSION     DAOOTK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_10218_cov_4.198662"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /gene="malQ"
                     /locus_tag="PK634_11215"
     CDS             complement(<1..1292)
                     /gene="malQ"
                     /locus_tag="PK634_11215"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOW98345.1"
                     /translation="MNFPRASGILLHPTSLPGPYGLGEIGPQARLFVDKLQAAKQQWW
                     QILPLGPTGYADSPYQSLSTFAGNPQWISFDLLVREGLISRSRLARFPEFPRDHVDFG
                     PVLIARNEVLQTACRSFDTRAGKSKREAFEHFCRKNADWLDDFALFFALKNAHELKPW
                     TTWEPELAQRDPAALRAAARKLRTAIRNVKIEQFLFADQWARLRKYCHKRGVQIVGDI
                     PIFVAHDSADVWAHPELFHLDERGFPTIVAGVPPDYFSATGQLWGNPLYRWDVHRQTG
                     YAWWVKRMRHIFEQVDIVRIDHFRGFEAYWEIPGRETTAINGRWVPGPGAELFNTLIR
                     ELGRLPIIAEDLGVITPAVEQLRDQFQFPGMRILQFAFGNDAKAADYRPESYPPNCAV
                     YSGTHDNDTTVGWFRSEPGKNSTRSREDIEKERRAILD"
     gene            complement(1394..1888)
                     /locus_tag="PK634_11220"
     CDS             complement(1394..1888)
                     /locus_tag="PK634_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOW98346.1"
                     /translation="MKHIIRQFFQREASPFVQFIKYALCGGMATLVDIVLFNFLAWKV
                     FPALGPEDPLVRLLGLDVAPVDDLTREHRYYIIKVITFFFGNLTAYILNVLWVFKPGR
                     HSRLKEILLFYLVSIISWGIGTVLGGMLIRFLGWETTVVYLVNMATSLMINFVCRKYI
                     IFKG"
     gene            complement(1908..2930)
                     /locus_tag="PK634_11225"
     CDS             complement(1908..2930)
                     /locus_tag="PK634_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOW98347.1"
                     /translation="MKTGIGLVLLLAGVAALGWPAFAAGTADRTTAPVEDPTNLVSTS
                     TSVPPVEAESPAGSEQPADTNVAPPVVVEPVEEEAPVKEKPARRGADLSDAFESSRGS
                     KFGLSAQQRSLKVPGKEGRPPREKTAWRWTLELGVDSTSGNKDTLRYDGSLGAQKETD
                     LHFFWFRAGGRYGKSEGERDTDNASAEARAERRLAERLYAALDGQVFQDRIAELSYRA
                     RANVSLGCFLVRSERTLLGLEAGPGYVQEKKGGEIDGYAAGRAAEYAERVLLSNLLAW
                     QAVEYVVSVEDANVFFMNAEVGLEALLSATLSLRVSLEDRYDGAPADDKEKNDLVTKT
                     ALKWAF"
     gene            3029..6328
                     /locus_tag="PK634_11230"
     CDS             3029..6328
                     /locus_tag="PK634_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOW98348.1"
                     /translation="MEPADLIVFMSEAARRQYIRRTALDRGFVDAERITTFARLRTDC
                     ARAARRAGLLQRPEADAVSRALLLRAAADEAAAAFAGKGVLGRAAPAALAEVLENMAD
                     TLAPLGHAAEAIQAWLLRGPAESKARQLGLLYETYRRRLDRAARADARDVNAAVLELL
                     HSPPSNWPAALARPGVGLHLRALTWLDPFAEQFVGALLERLGTQHVRLSSALPPAHAE
                     RMEDRLAARIRTEVLAGLDQEPWSSWTESLGDAWEVEDAQLAADSRDRLSFLRSAGLN
                     GEVEDLARRIRAEMDQHHVPPERIALVARQPDDYLDAVTRVFKEFGIPYFCRRRRPAA
                     DTAPVRVLMALLQFPLDPTRERLCALLTSPALAWPGWNSLSARQEAAEAILASGIGPV
                     LQAEDWRPPRGAARRIPDAVREPVQVVLSQMGLWNAPAGEFSLGDHARSALALADLLG
                     LVPGSLPPDGPAEREALDQARALLRRLEQESDAAGARHTRGAFLDLYGRLLADLAVPA
                     GSPEEYGVWIMSPADMAGLSFDVVFVAGLHEGSFPAVAREDAVFSDEERAAMHAALAR
                     HGVRLPQWSLPLSSIRSAQERFLFLSCIGAARERLVLSCQATDGEGRDLNPGDFFRSL
                     WALAGDSKDTSAPAGESWRVTLPLPECRTPGEARRRLGRLIERPSAARDSEPALLRKL
                     PAAPAAGNGERLARVIGVEHAREEFFNRTAGGRAAEGSHCGRLNSPAARRQIEGWLAG
                     RGALSPTSLEILARCRYRFLLGAVLGLGPARLREDAPDAMDRGRVIHDILDQVYRGLR
                     GDEGQPDALATDARAAFADLLRTRAWAVAEKPGAWVLRDRPGPSGARSLPLVAFDPGA
                     RDRCLDYIRAVADAVVLRAERAGLLLGNPGVWETEKPKLACIAENYVRLDMDLAGGER
                     RFPALLELPFGGRDGPPLRVGPGLGARGRVDRVDLVFDEGGRLSGLLVVDYKSPGRGG
                     QSKVAYAEEIAENLDCQLPVYAFAAQQALFGRCNEPELNAMTAAVFHIQDRDRKKMET
                     QFTNRRIGLGDDDSGSITGRFMERLAGNMERLRAADFAVDPLDCDLCDFRHICRVDVN
                     ALQSAAAGAGE"
     gene            6381..9902
                     /locus_tag="PK634_11235"
     CDS             6381..9902
                     /locus_tag="PK634_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOW98349.1"
                     /translation="MNVLFRASAGTGKTWQVSCLYTALVLGQPIETRGAGKRPARRHG
                     GRGLEPIPPGRIVLMTFTDHAAAELRLRVTARVLDARRRAVEEKRSADADLAARVLRA
                     LPGAPIGTIHSFCADLLRERALQAGLSPAFAILDEDSAHQLLEEAARDELLARLDRGR
                     AGRYDPDFEAFCQGMRALGSSRGTTVMQAAQSLLDQAASQGLDLAGAEGWLPAPRHAV
                     TPADFAAILEELRRVRAERGGKLPPKAAAVFQCLEKNLKIFPNIGKKSADISNVWKDA
                     DLEKFAAALEAEADLSFQGAGLQELSGRLRDAVASVQQTALYRVREGAVRAFARFAAG
                     VAAAYAARKRAVQGLDFDDLLIHTRDLLERDAAAAGRYDYLLLDEAQDTSRIQCDILR
                     RLWDPKVNRLVLCGDTKQSIYAWRYADPRVMPDLEAAQRATKSFRSIALRTSFRSKDV
                     ILDAVNRLFAGVYGARYTADDMLAPAPERSGDTAARGEGPGFELIEPDAAGGEAEDAP
                     DREARARAEMNSVAERIRLLVEGPADWQPRFRFNAAAGRFEEAGPLNRFRYADILILL
                     RSTTRQPLLEQALHRYGIPYRVGGRGRGLFARQEATDLLLLLKALAHPRDTLALLGFL
                     RSPWAGLSDEALLQLGWNGSGFDEAAFRRRALEGPLAGEPAAGPEDGERLASARALIR
                     KYAGQVDRRAPSAIVRDAIRDTGFDAVLAATFRGEQRLANLEKLLAWIEQAERGRLAA
                     ELASEMEQLVAEPPDLPEAALLDPDQNVVTLMTIHGAKGLTAGVVIVPELGSKTRNSH
                     AAALLESGADAGTGRLHVKAETVDRQEIATPGFGQAQEQADEVRKEEAKNLFYVALTR
                     ARDLVVLSGDPGSRKLSGWLADLRVFLADPEPSLVHRRTYGELRRATREAGLTADAAG
                     RARPWPDGWPAPGPLFDSIAAAYAPAPGPLPTLRYPATLLSACLHDPAAFRAPPPAER
                     EDDDDAAALGTAGHEMLEQAGQAGWGPSAEALATGPAAAALEPAARRELLARAQRAAI
                     RMKEEARGWDVATEWPFALKLEGGGATVIVDGTADLVLARAGRRRVVDYKFSEEDAAA
                     LAARYGLQLNLYRLAVARQAGVAPASVEASILAVRCEGVELVDIPFDPATAARAVAAA
                     RECYRSSRGAREGADQGAACAARTAAE"
     gene            complement(9863..>10218)
                     /locus_tag="PK634_11240"
     CDS             complement(9863..>10218)
                     /locus_tag="PK634_11240"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOW98350.1"
                     /translation="DARIAEHNTGSAAAGSSVPRLVFPGNRNDSANAILGYYLHKALI
                     DKTRAEDRGLRRARFVVLKNCVRPAVLLECGFLSNRNEESLMLTSAHREALALAIAKG
                     IMDYSAAVRAAQAAP"
BASE COUNT         1456 a         3609 c         3643 g         1510 t
ORIGIN      
        1 taatcgagga tcgcgcggcg ctccttctcg atgtcctcgc gcgagcgcgt gctgttcttg
       61 cccggctcgc tgcggaacca tccgaccgtg gtgtcgttgt cgtgcgtgcc ggaatagacc
      121 gcgcagttcg gcggatagct ctccggccgg tagtccgcgg ccttggcgtc gttgccgaac
      181 gcgaactgga gaatgcgcat gcccgggaac tggaactggt cgcggagctg ctccacggcg
      241 ggcgtgatca cgccgaggtc ctcggcgatg atcggcagcc ggccgagctc gcggatgagg
      301 gtattgaaca gctcggcgcc cgggccgggg acccaacgcc cgttgatcgc cgtcgtctcg
      361 cggcccggga tttcccagta ggcctcgaag ccgcggaagt ggtcgatccg cacgatgtcc
      421 acctgctcga agatgtggcg catccgcttg acccaccacg cgtagccggt ctggcggtgg
      481 acgtcccagc ggtagagcgg gttgccccag agctggccgg tcgcgctgaa gtaatccggc
      541 ggcacgcccg cgacgatggt cgggaaaccg cgctcgtcca ggtggaacag ctccgggtgc
      601 gcccagacgt ccgcgctgtc gtgggcgacg aagatcggga tgtcgccgac gatctggacg
      661 ccgcgcttgt ggcagtactt gcgcaggcgc gcccactggt cggcgaagag gaactgctcg
      721 atcttgacgt tgcggatcgc ggtgcgcagc ttgcgcgcgg ccgcccgcag cgcggccggg
      781 tcccgctggg ccagctccgg ctcccacgtg gtccacggct tcagctcgtg ggcgttcttc
      841 agggcgaaga acagcgcgaa gtcgtccagc caatccgcgt tcttccggca gaaatgctcg
      901 aacgcctcgc gcttgctctt gccggcgcgg gtgtcgaacg agcggcacgc ggtctggagc
      961 acctcgttgc gcgcgatgag gacggggccg aaatcgacgt ggtcgcgcgg gaactcgggg
     1021 aaacgcgcca gacgcgagcg cgagatgagg ccttcgcgaa cgagcaggtc gaagctgatc
     1081 cactgcgggt tgccggcgaa cgtcgagagc gactggtagg gcgaatccgc gtagccggtc
     1141 ggacccagcg gaaggatctg ccaccactgc tgcttcgcgg cctgcagctt gtctacgaac
     1201 agccgggcct gcggcccgat ttcaccgagc ccatacggcc ccggcagaga ggtgggatgc
     1261 aagaggattc cactggcccg cggaaagttc atgtcggcgt tgctcctgtg aacggttaaa
     1321 gggcgtgaaa cctagcagcc gtcggcgcgc aagtccacgc ttttccaacg ggccgcggcc
     1381 cgcccgctcc tccttatcct ttaaagataa tgtatttacg gcacacgaag ttgatcatga
     1441 gcgaggtcgc catgttcacc aggtagacga ccgtggtctc ccagcccagg aaccggatca
     1501 gcatcccgcc cagcaccgtc ccgatgcccc agctgatgat cgaaaccagg tagaacaaca
     1561 ggatctcctt caggcgcgaa tgccggccgg gcttgaatac ccagaggacg ttcagaatgt
     1621 aggcggtcag attgccgaag aaaaaggtga tgaccttgat gatgtaatac cggtgctccc
     1681 gggtcaggtc gtccaccggc gccacgtcca ggcccagcag gcggaccagc gggtcctccg
     1741 gccccagcgc cgggaacact ttccaggcga gaaagttgaa cagcacgatg tccaccagcg
     1801 tcgccatccc gccgcacagg gcgtacttga tgaactgcac gaaggggctt gcctcccgct
     1861 ggaaaaactg ccggatgata tgcttcatcg ttctccttgt ggccgcttca gaaggcccac
     1921 ttcagcgccg tcttggtcac caaatcgttc ttttccttgt cgtccgcggg tgcgccgtca
     1981 tagcggtcct cgagcgaaac gcgcaggctc aaggtggcgg acaggagggc ttccaagccg
     2041 acttccgcgt tcatgaaaaa gacgttcgcg tcctcgacgc tgacgacgta ctcgaccgcc
     2101 tgccacgcca gcaggttcga aagcagcacc cgctcggcgt actcggccgc ccggccggcc
     2161 gcatagccgt cgatctcccc gcccttcttc tcctgcacgt agcccgggcc ggcctcaaga
     2221 cccaggaggg tgcgctcgct ccggaccagg aaacagccca gggagacgtt ggcccgcgcc
     2281 cggtacgata gttcggcgat gcgatcctgg aacacctgcc cgtccagcgc ggcgtagagg
     2341 cgttcggcca ggcgtctctc ggcgcgcgcc tcggcggacg cgttgtccgt gtcgcgctct
     2401 ccctcgctct tcccgtaccg cccgcccgcc cggaaccaga agaaatgcag gtccgtctcc
     2461 ttctgcgcgc cgagcgagcc gtcgtaccgg agcgtgtcct tgttgccgct cgtgctgtcc
     2521 acgcccagtt ccagcgtcca gcgccaggcg gtcttctcgc ggggaggacg cccttccttg
     2581 ccgggcactt tcagggagcg ctgctgcgcc gagagcccga acttcgagcc gcggctgctc
     2641 tcgaaggcat ccgacaggtc agcaccgcgc cgcgcgggct tctccttcac cggcgcctct
     2701 tcctcgaccg gctccacgac gacgggcggc gccacgttgg tgtccgccgg ctgttccgat
     2761 cccgccggcg actccgcctc caccggcggg acgctcgtgg atgtcgaaac caggttggtc
     2821 ggatcctcga cgggggccgt ggtccggtcc gccgtgccgg cggcaaaggc cggccacccc
     2881 agcgccgcga cgccggccag gagcaggaca aggccgattc ccgttttcat ggcccggcac
     2941 gctatcggat tccccgccgc gccgtcaagc cgggcatcgc cgccactttc ctgttcgcaa
     3001 cggtccccgc cccttgtatg ctgcggacat ggagcctgcg gacctgatcg tcttcatgag
     3061 cgaggcggcg cggcgccagt acatccgccg gaccgccctc gaccgcggct tcgtcgacgc
     3121 ggagcgcatc acgaccttcg cgcggcttcg gacggattgc gcccgcgccg cccggcgcgc
     3181 gggccttctc cagcggcccg aagcggatgc cgtctcgcgg gccctgctcc tccgcgccgc
     3241 cgcggacgag gccgccgcgg ccttcgccgg gaagggcgtg ctcggtcgcg ccgcgccagc
     3301 cgcgctggcc gaggtcctgg aaaatatggc ggacacgctg gcgcccctcg gccatgcggc
     3361 ggaagccatc caggcctggc tcctccgcgg gcccgcggaa agcaaggccc ggcagctcgg
     3421 gctattgtac gagacctacc gccgccggct cgaccgcgcc gcgcgggcgg acgcgcgcga
     3481 cgtcaacgcg gccgtgctcg agctgctgca cagcccgccg tcgaactggc cggccgccct
     3541 cgcccggccc ggcgtcggtc ttcacctgcg ggccctgacg tggctggacc cgttcgccga
     3601 gcagttcgtc ggcgcgctcc tggaacggct cgggacccaa cacgtgcggc tttcctccgc
     3661 cctgcccccg gcccacgcgg aacggatgga ggaccggctc gcggcccgca tccgcacgga
     3721 agtcctcgcg ggcctggacc aggagccgtg gagttcgtgg accgaaagcc tcggcgatgc
     3781 ctgggaggtg gaggacgcgc aactcgccgc cgacagccgg gaccgcctgt cctttctacg
     3841 ctcggccggc ctgaacggcg aggtcgagga tctcgcgcgc cgcatccgcg cggagatgga
     3901 tcagcatcat gtgccgcccg aacggatcgc gctggtcgcc cgccagccgg acgactacct
     3961 cgacgcggtg acccgggtgt tcaaggagtt cggcatccct tacttctgcc ggcggcgccg
     4021 cccggcggcg gacacggccc cggtgcgggt gctcatggcg ctgctgcagt tcccgctgga
     4081 cccgacccgg gagcggctct gcgccttgct gacttcgccg gccctggcct ggcccgggtg
     4141 gaactccctg tcggcccggc aggaagcggc ggaggccatc ctggcctccg gtatcggacc
     4201 cgtgctacag gcggaagact ggcggccgcc gcgaggcgcg gcccggcgca tccccgacgc
     4261 cgttcgcgaa ccggtgcagg tcgtcctgtc gcagatgggg ctttggaacg ccccggccgg
     4321 cgagttctcc ctcggggacc atgcgcggag cgcgctggcg ctggcggacc tcctcggctt
     4381 ggtccccgga tcgctgccgc cggacgggcc ggccgagcgc gaggccctgg accaggcccg
     4441 ggcgctgctg cgccgcctcg aacaggagag cgatgcggcc ggcgcccgcc acacccgcgg
     4501 ggccttcctc gacctctacg gacgcctgct cgccgacctc gccgtgccgg caggctcccc
     4561 ggaggaatac ggggtatgga tcatgagccc ggcggacatg gcggggttga gcttcgacgt
     4621 ggtcttcgtc gccggcctcc acgaaggctc cttcccggcc gtcgcgcgcg aggacgccgt
     4681 gttcagcgac gaggagcgcg cggccatgca cgcggccctg gcgcggcacg gggtccggct
     4741 gcctcagtgg agcctgcccc tgtcatcgat ccgctccgcc caggaacgtt tcctattcct
     4801 gtcctgtatc ggggccgcgc gggaacggct ggtcctctcc tgccaggcga cggacggaga
     4861 aggccgcgat ttgaatccgg gcgatttctt ccgcagcctg tgggcgctcg cgggcgattc
     4921 caaggacacg tcggcgccgg caggcgaatc gtggcgcgtc accctgccgc tgcccgaatg
     4981 ccgtacgccc ggcgaggccc ggcgccgcct gggccgcctg atcgaacggc catccgccgc
     5041 gagggacagc gagcccgccc tcctccgaaa actgcccgcc gcgccggccg cgggaaacgg
     5101 cgaacggctg gcccgcgtca tcggcgtgga gcatgcgcgc gaggaattct tcaaccggac
     5161 ggcgggcggc cgcgcggcgg agggaagcca ctgcggccgg ctgaacagcc ctgccgcacg
     5221 ccggcagatc gaaggatggc tggccggccg cggcgcgttg agcccgacct cgctggagat
     5281 cctcgcccgt tgccgctacc gcttcctgct gggcgcggtc ctcgggctcg ggcccgcccg
     5341 gcttcgcgag gatgcgccgg acgcgatgga ccgcgggcgg gtcatccacg acatactcga
     5401 ccaggtgtac cgcggcttgc gcggcgacga gggccagccg gacgcgctgg cgaccgacgc
     5461 gcgcgccgcg ttcgcggacc tgctccgaac gcgggcttgg gcggtggcgg agaagcccgg
     5521 cgcgtgggtc ctccgggatc ggcccggccc gtcgggcgcg cgctcgctgc cgctcgtcgc
     5581 gttcgatccc ggcgcacgag accgctgcct cgactatatc cgagccgtgg cggacgccgt
     5641 cgtgctccgg gcggagcgcg cgggcctgct gctggggaat cccggcgtct gggaaaccga
     5701 gaagcccaag ctcgcgtgca tcgcggagaa ctacgtgcgg ctcgacatgg acctggccgg
     5761 cggcgagcgg cgctttcccg cgctgctgga gctccctttc ggaggccggg acgggccgcc
     5821 gctgcgcgtg ggccccgggc tcggcgcacg cggccgcgtg gaccgggtgg acctggtgtt
     5881 cgacgagggc ggccgcctct ccggcctgct ggtggtggat tacaagagcc cgggccgcgg
     5941 ggggcagtcg aaggtggcgt atgccgagga gatcgccgag aacctcgact gtcagctgcc
     6001 ggtgtacgcc ttcgcggcgc agcaggcgct gttcgggcgc tgcaatgagc cggagctcaa
     6061 cgcgatgact gccgccgtgt tccacatcca ggaccgcgac cggaaaaaga tggaaaccca
     6121 gtttaccaat cggcggatcg ggctgggcga cgacgattcg ggctcgatca ccggccggtt
     6181 catggagcgg ctggccggga acatggagcg cctgcgggcc gccgatttcg cggtggaccc
     6241 gctggactgc gacctgtgcg atttccgcca tatctgccgc gtggacgtca acgccctgca
     6301 gagcgcggcc gccggcgccg gcgaatgaag accatgcgaa catattgctt cacggccgtg
     6361 aatagttttg ttacgatacc atgaacgtcc tcttccgagc ctcggcgggc acgggcaaga
     6421 cctggcaggt ctcgtgcctc tacaccgccc tcgtgctcgg ccagccgatc gagacccgcg
     6481 gcgccgggaa acgcccggcg cggcgccacg gcggccgggg cctcgagccg atcccgcccg
     6541 gccggatcgt gctcatgacc tttaccgacc acgccgccgc cgagctgcgc ctccgcgtga
     6601 ccgcccgcgt cctggacgcc cggcgccggg ccgtggagga aaagcggtcg gcggacgccg
     6661 atctcgcggc ccgggtcctc cgcgccctgc ccggggcgcc gattggcacc atccattcct
     6721 tctgcgccga tctcctgcgc gaacgcgccc tccaggccgg cctctccccc gccttcgcca
     6781 tcctcgacga ggattccgcc catcagctgc tcgaggaggc ggcgcgcgac gaactgctgg
     6841 cgcgcctgga tcgcggccgg gccgggcgat acgatcccga tttcgaggcc ttctgccagg
     6901 ggatgcgcgc cctcggctcc tcgcgcggca cgacggtcat gcaggcggcc cagtccctgc
     6961 tggaccaggc cgccagccag ggcctcgacc tcgccggggc cgagggttgg ctcccggcgc
     7021 cgcgccacgc ggtcacgccg gcggacttcg ccgcgatcct ggaggaactg cggcgggtcc
     7081 gggccgagcg cggagggaag ctgccgccca aggccgcggc ggttttccaa tgtttggaaa
     7141 aaaatctgaa aatttttcca aacattggaa aaaaatccgc agatatttcc aatgtttgga
     7201 aagacgcgga cctcgagaaa ttcgccgccg ccctcgaagc cgaggccgac ctgtcgttcc
     7261 aaggcgcggg gctccaggag ctgtccgggc ggctgcggga cgccgtggct tccgtgcagc
     7321 agacggcgct ctacagggtc cgcgagggcg cggtgcgggc gttcgcgcgc ttcgcggccg
     7381 gcgtcgcggc cgcctatgcc gcgcgcaagc gggccgtcca gggtctggat ttcgacgacc
     7441 tgctgatcca tacccgggac ttgctggaac gggatgccgc cgcggcgggc cgctacgact
     7501 acctcctgct cgacgaggcc caggacacgt cccgcatcca gtgcgatatc ctgcgccggc
     7561 tctgggaccc gaaggtcaac cgcctggtcc tgtgcggcga taccaagcag tcgatctacg
     7621 cgtggcgcta cgccgatccc cgcgtcatgc ccgacctgga ggcggcgcaa cgggccacga
     7681 agtcgttccg gtcgatcgcc ctgcgcacga gttttcgcag caaggacgtc atcctcgacg
     7741 ccgtcaaccg gctgtttgcc ggggtctacg gcgcacgtta cacggcggac gacatgctcg
     7801 cgcccgcgcc cgagcggagc ggcgacacgg cggcccgcgg cgaggggccg ggcttcgaac
     7861 tgatcgagcc ggacgcggcg ggcggggaag ccgaggacgc gccggaccgg gaggcccgcg
     7921 cccgggccga gatgaactct gtcgccgaac gcatccgcct gctcgtcgaa gggccggcgg
     7981 actggcagcc gcggttccgg ttcaacgctg ccgccgggcg tttcgaggag gcggggcccc
     8041 tcaaccgttt ccggtacgcg gacatcctga tcctcctgcg cagcacgacc cggcagccac
     8101 tgctcgagca ggccctgcac cggtacggga ttccctaccg cgtcggcggg cgcgggcgcg
     8161 gccttttcgc ccggcaggag gccacggacc tcctgctgct gctgaaggcg ctcgcgcatc
     8221 cgcgcgacac gctggccctg ctcggctttt tgcgctcgcc ctgggcgggg ctttccgacg
     8281 aggcgctgct ccaactcggg tggaacggat cgggatttga cgaggccgcc ttccgccggc
     8341 gcgccctcga aggcccgctt gccggcgagc cggcggccgg cccggaggac ggggagcggc
     8401 tggcctcggc gcgcgcgctg atccgaaagt acgccgggca ggtggaccgg cgcgcgccgt
     8461 cggcgatcgt ccgcgacgcg atacgggata cgggcttcga cgccgtcctg gccgccacgt
     8521 tccgcggcga gcagcgcctg gccaacctgg agaaactcct ggcctggatc gagcaggccg
     8581 agcggggccg gctggccgcg gagttggcgt cggagatgga gcagctggtg gccgagccgc
     8641 cggatctgcc ggaggccgcc ctgctcgatc ccgaccagaa cgtggtcacg ctcatgacga
     8701 tccacggggc caagggcctg accgcgggag tggtgatcgt gccggagctg ggatcgaaaa
     8761 cgaggaattc gcacgcggcg gcgttgctgg agagcggggc cgacgcaggc acggggcggc
     8821 tccacgtgaa agcggaaacc gtggaccgcc aagagatcgc cacgccggga ttcggacagg
     8881 cgcaggagca ggcggacgag gtacggaagg aggaggcgaa gaacctgttc tacgtggccc
     8941 tgacccgggc gcgcgacctc gtggtgctct cgggggatcc gggttcgcgg aagctgtcgg
     9001 gctggctggc cgacctccgc gtcttcctgg ccgacccgga accctccctg gtccaccggc
     9061 ggacctacgg tgaattgcgc cgggccaccc gggaggccgg cctgacggcg gacgcggccg
     9121 gccgggcccg tccctggccg gacggctggc cggcgcccgg gccgctgttc gactcgatcg
     9181 cggcggccta tgcccccgcc cccggcccgc tcccgacgct gcgctatccg gccacgctgc
     9241 tttcggcctg ccttcacgat ccggccgcct tccgggcgcc cccgcccgcg gagcgcgagg
     9301 atgacgacga tgcggccgcc ctcgggaccg cgggtcacga gatgctcgag caggccggcc
     9361 aggccggctg gggcccgtcc gcggaggcgc tcgcgaccgg ccccgccgcc gccgcgctgg
     9421 agccggcggc ccggcgcgaa ctgctcgcgc gcgcgcaacg ggcggcgatc cgcatgaaag
     9481 aagaagcgcg gggttgggac gtcgcgacgg aatggccgtt cgcgctgaag ctggagggcg
     9541 gcggcgcgac ggtcatcgtg gacggtacgg cggacctggt cctggcgcgc gcgggccggc
     9601 gccgggtggt ggactacaag ttcagcgagg aggacgcggc cgcgctggcg gcgcggtacg
     9661 gcctgcagct caacctgtac cggctggccg tcgcccggca ggccggcgtc gcgcccgctt
     9721 ccgtggaggc ctcgatcctg gccgtccggt gcgaaggcgt ggaactcgtg gatattccct
     9781 tcgatccggc gacggcggcc cgcgccgtgg cggccgcgcg ggaatgctac cgctcgtcgc
     9841 ggggcgcccg ggaaggcgcg gatcagggcg cggcctgcgc cgctcggacg gccgcggagt
     9901 aatccatgat gcccttggcg atggccagcg ccagcgcctc gcggtgcgcg gaggtcaaca
     9961 tgagggactc ctcgttcctg ttggagagga atccgcattc gagcagcacc gccggccgga
    10021 cgcagttctt gagcacgacg aagcgggcgc gccggaggcc ccggtcctcc gcgcgggtct
    10081 tgtcgatcag ggccttgtgg agatagtagc cgagaatggc gttggccgag tcgttgcggt
    10141 tgccggggaa gaccaggcgc gggacggagg agccggcggc ggcggaacct gtgttgtgtt
    10201 cggcgatgcg ggcatcgg
//
LOCUS       DAOOTK010000111        10086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4340_length_10086_cov_4.791147, whole genome shotgun sequence.
ACCESSION   DAOOTK010000111 DAOOTK010000000
VERSION     DAOOTK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_10086_cov_4.791147"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1756)
                     /gene="pabB"
                     /locus_tag="PK634_11245"
     CDS             complement(26..1756)
                     /gene="pabB"
                     /locus_tag="PK634_11245"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOW98351.1"
                     /translation="MIEAWHHSFSRTGGWDVRLADPCEVQSADELPDVLPLVRRAEAA
                     AREGKWVTLLLSYESAPAFDAALVTREPGAFPLAWMAVFEKIRGSGPPPAEGAAPAPW
                     QPLVSRDEYESAIRRIREYIAAGDTYQVNYTFPLRAAFPGDGAAWFLRQGPAQGAPWS
                     VFVDTGRFQVLSFSPELFFEWTADRLVCRPMKGTAQRGLWFEDDEARRRALSESRKDR
                     AENLMITDMVRNDLGRIAEVGSVKTARLFEIERYPTLFQMISEIEARPRPGIRLEDVL
                     RALFPGASVTGAPKVRTMQIIRELEPQRRGLYTGAVGLLRPGGAAQFSIPIRTVVADR
                     AAGEAVFHAGGGVTWDSTAEGEYDECLLKTAFLHESPRPFSLLETLRLEDGEYYLLER
                     HLRRLRQSAVYFELPVDESCVRTRLAQLAADHRAGVWRARLRLDARGEIEIEAQPLIP
                     DEEPWRVALADEPVNSRDPFLYHKTTRREIYDRRLAAHPGCRDVILWNERGEITESCL
                     ANVSAVIGGREYTPPVGCGLLPGTLRGEWLESGRLAERVIRIEEWRQAEAVHLFNSVR
                     GRIRIEPSHG"
     gene            complement(1753..3006)
                     /gene="bioA"
                     /locus_tag="PK634_11250"
     CDS             complement(1753..3006)
                     /gene="bioA"
                     /locus_tag="PK634_11250"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOW98352.1"
                     /translation="MQDARHVWHPYTRMSAVDAGAPPSIVRGEGIYLFDEAGCRYTDA
                     IASWWCCALGHGHPRIVEAIRRQAGELQHSILGNLSHPRAEELAERLAQLMPSPDRHV
                     LFASDGASAVEAALKIALQFRTNTGQSSKRRFAYLAEAYHGDTLGAVSVGYLENFHKP
                     FESVLFPAFEAPVPLSAEQEDACFKKTAALLAKHRDDLTALIVEPLCQGAAGMRMYSP
                     AYLRRLAELCRENDVLLIADEIATGFGRTGRMFAFEHAGIDPDIVCVGKALSAGYLPI
                     SATIVKDAVYRTFSDTPDDHSFYHGHTFCGNPVAAAAALEALRVYKEARIWEKAAALE
                     HVFRDVLEPLKTRPGVKGVRVLGAIAAVELNSVDAAWAVRDRLLARRILARPLGAVVY
                     LMPPLNIGREVATELARSLLDAITA"
     gene            complement(3046..4623)
                     /locus_tag="PK634_11255"
     CDS             complement(3046..4623)
                     /locus_tag="PK634_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98353.1"
                     /translation="MKKFVACLMVATAAACSAQDFMCETFDGYLLGPVSSYSGWGQTN
                     DPPILTNTQPAQVTNVRPATSPNSLLFAPPSAGKQCTLAMYTNLNAYYSYTDPNHPVL
                     RTSFMLYKENARQNFAFLQGLANTEMGLLTDTNDGTIIVNATDTGIPFVTGRYAEVVY
                     LYNMSNNDSCVQYDGTNIADWTGADAPSLTQIVYTAFYRLPDPYAGAPLYVDQVHVDK
                     PTPGTVAWWRFDTAGTNSAGDHAGLFKAARLVSMDTTPKPPASTPLYDGWQDRSDAQG
                     RRSFKLDELNPWRDTGKMPEWTLEAIMAAEPGAVDLPSNFQLLHWGTGIGGGSSTNSE
                     INFVWLRLTGQFSAYLRDAQQTNNSIQYLTPLGRMPADGQWHHVAFVKTGSWLHVYTD
                     YRPTIQYPLDAASTGYYSFATSSHAGIGVSLNNGNHANTNQWLDELRFTARALPSSEF
                     LQWGQPLMVSAPDPAAPSWNLDVLSISGRTYNVQTAPSPGSSTWSDVLPGAIATGHYS
                     TLTIPATTNRILRAVRQ"
     gene            4718..6307
                     /gene="lnt"
                     /locus_tag="PK634_11260"
     CDS             4718..6307
                     /gene="lnt"
                     /locus_tag="PK634_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOW98354.1"
                     /translation="MNGALKEKLHAALPRAGAAASGLLLFAAFPPLEWRNAAWVALAP
                     LLLAARFVSVRRAAFCALLAGALFWLASIAWLTRVTVVGWIILALYSALYFVPPAVLM
                     SAWSARRGVRSGWSNLALMLLLAATWTGAEYLRATLFTGWPWNPLGATQYANLPLIQL
                     ARGGGVYAVSALVVWVNAALALTILRYLDVRGHWGRRPHPELMLGFLAVALAVVYGWR
                     SVRADRAEGLPLRVAVIQPNIPQDEKWTAEKFEMIYGRLRTLTEQALRVGGSDLVVWP
                     ETAVPDDIRDSEASYGLAVEMVTNGAPLLVGSMDTEWLDEGPRYYNSSFLFDTNGMIV
                     KGYDKRHLVIFGEYVPLQPFLPFVRALTPIQASFSPGHTSTVFRLDRPDVPFSALICF
                     EDTVAPLAAESVRNGARVLFNQTNDAWFDPGWAPKQHMIQCVFRSVENAVPSVRCANT
                     GISCFIDRRGRVHDVLDDGNGFTRVMGIRAAEVRVPPEDMPLTFYTRHGDLFAQACAG
                     FGALALFFALRRGRDAGKKSE"
     gene            6390..7515
                     /gene="prfB"
                     /locus_tag="PK634_11265"
     CDS             join(6390..6458,6460..7515)
                     /gene="prfB"
                     /locus_tag="PK634_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_160627583.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOW98355.1"
                     /translation="MLYELQQQWQTLQKRFEEMRGYLDVETRRRSLAELEALAAAPEF
                     WNDQARARDVIARTNAVRGVLEPYEKAERDIEDTGVLLELAEQEKDPAQQEPVLHEIE
                     GHLKRIEADFSALEIRLLLSGRFDANNAYLSLHAGAGGTESCDWTSILFRMYRRYCED
                     HGFEVEIMDSLSGEEAGLKSITFLVRGPYAYGYFRAERGVHRLVRISPFDANKRRHTS
                     FASLDVIAEVDDSINIEIGENDLRVDTYRSGGAGGQHVNKTDSAVRLVHLPTGIVVAC
                     QAERSQHKNRARAMKMLMAKLYEYEADKKRKEMEKFYGEKGEIAWGRQIRSYVLQPYT
                     MVKDHRTDVETGNADAVLDGDLDPFIQAYLRQNPGTSEGP"
     gene            7547..8335
                     /gene="trpC"
                     /locus_tag="PK634_11270"
     CDS             7547..8335
                     /gene="trpC"
                     /locus_tag="PK634_11270"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOW98356.1"
                     /translation="MNILAEIIAHKRAELAEWKQARSVEMLRAVQVAPPPPFAAALRA
                     APIGLIAEAKRRSPSAGAIREPFDPAAIAAAYARAGAQAMSVLMDRRYFAGGEEAFRA
                     VRASVSLPLLYKEFVVDPWQVWHARALGASAVLLIAAALDDRELAALLTECRAAGLEV
                     LLEVHDETEMRRAAASGAECIGVNNRDLRTFTVDLGTTERLKTLAPTRCTLVSESGIR
                     TAADVLRLQAAGVQAVLVGEHLLRQPDLERAVRELMGLAWEGES"
     gene            8332..8520
                     /locus_tag="PK634_11275"
     CDS             8332..8520
                     /locus_tag="PK634_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98357.1"
                     /translation="MKTVGILSGLVAFVLCAAAGLWILTKVGFDHGDDVMSTGIGLYF
                     IGKAFFVGPMLILAALKH"
     gene            8608..9246
                     /locus_tag="PK634_11280"
     CDS             8608..9246
                     /locus_tag="PK634_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOW98358.1"
                     /translation="MKICGLCSAADVRAVAALKPDALGFVFWPGSKRCVRAADVATWT
                     RDLPPGLLKVGVFVNAAPAELEATVRKAGLDVVQYHVFQTLEKESANFPDIGKNIRLF
                     SKHWKTVHLDRGGPPPIESAFVDAFLVDSYSADSPGGTGRLGNWAAARAFVERCGKPV
                     VLAGGLTPENVAEAIRTVRPWGVDVSSGVEIAPGRKDLKKVEEFIRACRENA"
     gene            9228..>10086
                     /gene="trpB"
                     /locus_tag="PK634_11285"
     CDS             9228..>10086
                     /gene="trpB"
                     /locus_tag="PK634_11285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOW98359.1"
                     /translation="MPRKRIDTEPDAHGHFGPYGGMFVPETLMAPLQDLEREFRAARR
                     DPSFRRDVEYYLREYVGRPTPLYFAERMTRELGGPRIYLKREDLCHTGAHKINNCIGQ
                     CLLARRMGKKRVIAETGAGQHGVATATCAALFGMQCVVYMGKVDMERQALNVFRMRSL
                     GAEVVPVTAGQQTLKEAISEAMRDWVTNVRTTHYVLGTAYGAHPYPVMVRTFQSVIGR
                     EARRQILKQEGRLPSALVACVGGGSNAIGLFYPFLQDRGVEMIGVEAGGLGIRSGKHA
                     ARFADGRVGV"
BASE COUNT         1671 a         3297 c         3404 g         1714 t
ORIGIN      
        1 cggatactat ttccgatcct cacggctatc cgtgggaagg ctcgatccgg attcggcccc
       61 gcacggagtt gaagagatgc acggcttcgg cctgccgcca ttcctcgatc cggatcaccc
      121 gttcggcgag acggccggat tccagccatt ccccgcgcag ggtgcccggc agcaatccgc
      181 acccgacggg cggcgtgtac tccctcccgc cgatgaccgc cgacacgttg gccaggcagg
      241 attccgtgat ctctccgcgt tcgttccaga ggatgacatc acggcaaccc ggatgcgccg
      301 ccaggcgccg gtcgtagatt tcgcgccgcg tggttttgtg atacaggaac ggatcgcggc
      361 tgttcaccgg ttcgtctgcc agcgcgacgc gccacggctc ctcgtcggga atcaggggtt
      421 gagcctcgat ctcgatttcg ccacgggcat cgagccgcag ccgcgcccgc cagaccccgg
      481 ccctgtgatc cgcggccaat tgggccaacc tggtcctaac acaggattcg tcgaccggaa
      541 gttcgaaata gacggcggat tgacgcaaac gccgcaggtg cctttccagc aggtagtatt
      601 cgccgtcctc caggcgcagg gtttcaagca gcgagaaggg acgcggggat tcgtggagga
      661 aggcggtctt cagcaggcat tcgtcgtatt cgccctccgc cgtcgaatcc cacgtgacgc
      721 cgcctccggc atggaatacg gcttcgccgg ccgcgcggtc cgcgaccacc gttcggatcg
      781 gtatgctgaa ttgcgcggcg ccgcccggac gcagcagccc gacggcgccg gtgtacagcc
      841 cgcgcctctg cggctccagt tcccggatga tctgcatcgt gcggaccttc ggcgcgccgg
      901 tgaccgacgc gccgggaaag agcgcccgca gcacgtcctc caaccggatc ccgggccgcg
      961 gccgcgcctc gatctcggag atcatctgga aaagggtggg gtagcgctcg atctcgaaga
     1021 gccgcgccgt tttgacggag ccgacctccg caatccgccc gagatcgttc cgaaccatgt
     1081 cggtgatcat caggttctcg gcgcggtcct tccggctctc cgacagggcc cggcgccgcg
     1141 cctcgtcatc ctcgaaccag agaccgcgct gcgccgtgcc tttcatcggc cggcatacga
     1201 gacgatcggc cgtccactcg aaaaacagct ccggcgagaa gctcaatacc tggaagcggc
     1261 cggtatccac gaaaacgctc cagggcgcgc cctgggccgg tccctgccgc aggaaccagg
     1321 cggcgccgtc gcccgggaag gccgcccgca gcgggaacgt gtagttcacc tggtacgtgt
     1381 cgccggcggc gatgtattcc cggatccgcc ggatggcgga ctcatactcg tcgcgggata
     1441 ccagcggttg ccagggagcc ggggcggcgc cttcggccgg cggagggccg ctgccgcgga
     1501 tcttctcgaa aacggccatc caggccagcg gaaacgcgcc cggctcgcgc gtgaccagcg
     1561 cggcgtcgaa ggccggggcg gattcataac tcaacagcag cgtcacccac tttccctcgc
     1621 gcgcggcggc ctcggcgcgg cggaccagcg gaagcacgtc cggcagctcg tcggccgatt
     1681 ggacctcgca ggggtcggcc agccggacgt cccatccgcc cgtccgggaa aaggaatgat
     1741 gccaggcctc gatcatgccg tgatggcgtc gagcagcgag cgggccagtt cggtcgccac
     1801 ttctcgtccg atgttcagcg ggggcatgag gtacaccacg gcccccagcg gccgggccag
     1861 gatgcgacgg gccagcaggc ggtcgcggac ggcccaggcg gcatcgacgg aattcagttc
     1921 caccgccgcg atcgccccaa gcacgcggac ccccttcacg ccgggccgcg tcttcaaggg
     1981 ctccagcacg tcccggaaga cgtgttcgag ggcggcggcc ttttcccaaa tgcgcgcttc
     2041 cttgtacacc cgcaaggcct ccagggctgc cgcggcggcg accggattcc cgcagaacgt
     2101 gtggccgtgg tagaaagagt gatcatcggg agtatccgag aacgtccggt agacggcatc
     2161 cttgacgatg gtcgcgctga tggggagata gcccgccgag agcgctttcc cgacgcagac
     2221 aatgtccggg tcgatccccg cgtgctcgaa cgcgaacatc cggccggtgc gcccgaagcc
     2281 cgtcgcgatt tcatcggcga tcagcaggac atcgttttcg cggcaaagct ccgccaaccg
     2341 gcggaggtag gcgggggagt acatcctcat gcccgccgcg ccctggcaca ggggttcgac
     2401 aatcagggcg gtcaagtcgt cgcggtgctt cgcaaggagg gcggccgttt tcttgaaaca
     2461 agcgtcctcc tgctccgccg acagcgggac gggcgcctca aaagccggga acagaaccga
     2521 ctcaaaaggc ttgtgaaaat tctcgagata gccgaccgac acggcgccga gcgtgtcccc
     2581 gtggtaggcc tctgcgaggt aggcgaagcg ccgtttggag gattggccgg tattcgtcct
     2641 gaactggagc gcgattttca gcgccgcctc gaccgcgctc gcgccgtcgg aggcgaagag
     2701 aacatgccgg tccggcgaag gcatcagttg cgccaggcgc tcggccagtt cctcggcccg
     2761 cggatgggag aggttgccga ggatgctgtg ctggagttcg cccgcctgcc gccggatggc
     2821 ctcgacgatc cggggatgcc cgtgccccag cgcgcaacac caccatgacg cgatggcgtc
     2881 ggtgtagcgg catcccgcct cgtcaaacag atatatccct tcgccccgta cgatggaagg
     2941 gggcgcgccg gcgtccacgg cggacatccg cgtgtacggg tgccatacgt ggcgggcgtc
     3001 ttgcatgttc ctgtgcctca tggaagcggg tggcccgaat ccgtcctact gccgaacggc
     3061 gcggaggatc cggttcgtgg tggccgggat ggtcagggtg ctgtagtggc ccgtggcgat
     3121 cgctcccggc aggacatcgg accacgtcga agatcccggc gagggcgccg tctgcacgtt
     3181 gtaggtgcgg ccggaaatcg agagcacgtc gaggttccag ctgggggcgg cgggatcggg
     3241 cgcggatacc atcagcggct gcccccactg aaggaactcg ctggaaggaa gggcccgcgc
     3301 ggtgaagcgc agttcgtcga gccactggtt ggtgttggcg tggttcccgt tgttgaggct
     3361 gaccccgatg cccgcgtgac tgctggtcgc gaagctgtag taacccgtgg aggccgcatc
     3421 cagcgggtat tggatggtcg gccggtaatc cgtgtacacg tggagccagc ttcccgtctt
     3481 gacgaaggcg acgtggtgcc actgtccgtc cgcgggcatc cggcccaggg gggtcaggta
     3541 ttggatgctg ttgttcgtct gctgcgcgtc gcgcaggtat gccgaaaact ggcccgtcag
     3601 cctcaaccag acaaagttga tttccgagtt ggtcgaactg ccgccgccta tgccggtgcc
     3661 ccagtgcagc aattggaagt tgctgggaag atccaccgcc ccgggttccg cggccatgat
     3721 ggcctcgagc gtccactcgg gcatcttgcc cgtgtcccgc cacgggttga gttcgtccag
     3781 cttgaaggat cgccggccct gggcatccga acgatcctgc cacccgtcgt agagaggcgt
     3841 cgaggcgggg ggcttgggcg tggtatccat gctcaccaac cgggcggcct tgaagaggcc
     3901 ggcatggtcg ccggcggaat ttgtcccggc cgtgtcgaag cgccaccagg ccaccgtgcc
     3961 gggcgtgggc ttgtccacat ggacctggtc cacgtagagc ggcgccccgg catacgggtc
     4021 cggcagccgg taaaaggccg tgtatacgat ttgcgtcagg gagggggcgt ccgcgcccgt
     4081 ccagtcggcg atgttggtcc cgtcgtactg cacgcagctg tcgttgttcg acatgttgta
     4141 gagatagacg acctcggcgt agcggcccgt gacgaacggg atgccggtgt cggtcgcgtt
     4201 tacaatgatg gtcccgtcat tcgtgtccgt gagaaggccc atttccgtgt tggccaggcc
     4261 ctgcaagaag gcgaaattct gccgcgcatt ttccttgtac agcatgaacg aggtccgcag
     4321 cacgggatga ttcgggtcgg tgtaggaata gtacgcgttc agattggtgt acatggccag
     4381 cgtgcactgc ttgccggcgg acggaggagc gaacaggagg ctgttgggac tggtggccgg
     4441 gcgcacgttg gtgacctgtg cgggctgggt gttggtgagg ataggcgggt cgttggtctg
     4501 gccccagccc gaataggacg agacgggccc cagcaggtag ccgtcgaacg tctcgcacat
     4561 gaaatcctgc gcggagcacg cggcagccgt cgcaaccatc aggcaggcaa caaatttctt
     4621 catggttatc ccccctttat gaagtgtagc agaatcggct tcatatcgtc aaagttgcga
     4681 tggcagggat ttccgctatt cttccgcgtc tcgaaccatg aatggcgctc tcaaggaaaa
     4741 actacatgcg gccctcccgc gggccggcgc ggcagcgagc gggctgctgc tcttcgcggc
     4801 ctttccgccg ctggagtggc ggaacgcggc ctgggtggcg ctcgcgcccc ttctgctggc
     4861 tgcccgcttc gtatccgttc gccgggccgc gttctgcgcc ctcctggccg gagccctgtt
     4921 ctggctggcc tccatcgcgt ggctcacgcg cgtgaccgtg gtcggctgga tcatcctggc
     4981 gctctacagc gcgctctact tcgtcccgcc cgcggtgctg atgtccgcct ggtcggcgcg
     5041 gcgcggcgtc cgctccggct ggagcaacct cgcgctcatg ctcctgctgg ccgcgacgtg
     5101 gacgggggcg gagtacctgc gcgccacgct cttcaccggc tggccctgga atccgctggg
     5161 cgccacccag tacgccaacc tgccgctgat ccagctcgcg cgcgggggcg gtgtctatgc
     5221 cgtctccgcg ctcgtggtct gggtgaatgc cgccctggcc ctgacgatcc tccgctacct
     5281 ggacgtccgg ggccattggg gccgtcgccc gcatcccgag ctgatgctgg gcttcctcgc
     5341 cgtcgccctc gcggtcgtgt acggctggcg gagcgtacgc gcggatcgcg cggaaggcct
     5401 gccgctgcgc gtcgccgtga tccagccgaa catcccccag gacgagaagt ggaccgccga
     5461 aaaattcgaa atgatctacg ggcgcctgcg gacgctgacc gagcaggccc tgcgcgtcgg
     5521 cggatcggac ctcgtcgtct ggcccgaaac cgcggtcccc gacgacatcc gcgacagcga
     5581 ggccagttat ggcctcgctg tcgaaatggt caccaacggc gcgccgctgc tggtcggttc
     5641 gatggacacg gaatggctgg acgagggccc gcgctactac aacagctcct tcctcttcga
     5701 caccaacggc atgatcgtca aaggctacga caagcgacac ctcgtgatct tcggcgagta
     5761 cgtgccgctg cagccgttcc tgccgttcgt ccgggcgctc acgccgatcc aggccagctt
     5821 ctcgccgggc cacaccagca cggtgttccg gctggatcgc ccggatgttc ccttctcggc
     5881 gctcatttgc ttcgaggaca cggtggcccc gctggccgcc gagtccgttc gaaacggggc
     5941 ccgcgtcctc ttcaaccaga ccaacgacgc gtggttcgac cccggctggg cgccgaaaca
     6001 gcatatgatc cagtgcgtct tccgcagcgt ggagaatgcc gtgccgtccg tccgctgcgc
     6061 caacacgggg atttcctgct tcatcgaccg gcgcggccgc gtccacgacg tgctcgatga
     6121 cgggaacgga ttcacgcgcg tgatgggcat ccgcgcggcc gaagtccgcg tgccgccgga
     6181 ggacatgccc ctgacgttct atacccggca tggcgacctc ttcgctcaag cgtgcgcagg
     6241 gttcggagcc ctggcgctgt tttttgcttt acggcgcggc cgggatgcgg gaaagaagag
     6301 tgaatagtga atggtgatta gtgaatagta gcctgaaccc tgaaccctgc ttgcccgccg
     6361 gaggcccggc gtaggcggga accctcccca tgctctacga actccagcag caatggcaaa
     6421 ccctccagaa gcgtttcgag gagatgcggg ggtatctttg acgtcgaaac ccgccgccgc
     6481 tcgctagccg aattggaagc cctcgccgcc gcgccagaat tctggaacga ccaggctagg
     6541 gcccgggacg tcatcgcccg caccaacgcc gttcgcggcg tgctggagcc ctacgagaag
     6601 gccgagcggg atatcgagga caccggcgtg ctcctggaac tggccgagca ggagaaagat
     6661 ccggcccagc aggaacccgt ccttcacgaa atcgagggcc atctcaagcg gatcgaggcg
     6721 gatttctccg cgctggagat ccggctgctc ttgagcgggc gcttcgacgc caacaacgct
     6781 tacttgagcc tccacgccgg cgcgggcggg accgagtcct gcgactggac cagcatcctg
     6841 ttccgcatgt accgccgcta ctgcgaggac cacggcttcg aggtcgagat catggactcg
     6901 ctctcgggcg aggaggccgg cctcaagagc atcaccttcc tcgtccgcgg cccctacgcc
     6961 tacggctact tccgcgccga gcgcggcgtc catcgcctgg tccgcatcag ccccttcgac
     7021 gcgaacaagc gccgccacac gtccttcgcc tcgctcgacg tcatcgcgga agtcgacgat
     7081 tccatcaata tcgagatcgg tgaaaatgac ctgcgcgtgg acacctaccg gtccggcggc
     7141 gccggcggcc agcacgtgaa caagaccgac tccgccgtgc gcctcgtgca cctgcccacc
     7201 ggcatcgtgg tcgcctgcca ggccgagcgt tcgcagcaca agaaccgcgc ccgggccatg
     7261 aagatgctga tggccaagct ctacgagtac gaggcggaca agaaacgcaa ggagatggag
     7321 aagttctacg gcgagaaggg cgagatcgcc tggggccggc agatccggtc ctacgtgctc
     7381 caaccgtaca cgatggtcaa ggaccaccgg accgacgtcg agaccggcaa cgccgacgcc
     7441 gtcctcgacg gcgacctcga tccattcata caggcctatc ttcgccagaa tccaggaacg
     7501 agtgaggggc cgtaacgtga cccgggcctc cggcccgtga gctgccgtga atatcctggc
     7561 tgaaattatc gcgcacaaga gggcggaact ggccgaatgg aaacaagccc ggtccgtgga
     7621 gatgcttcgc gccgtgcagg tcgccccgcc gccgcccttt gccgccgcgc tgcgcgccgc
     7681 gccgatcggc ctgatcgccg aggccaagcg ccggtcgccg tcggcgggcg ccatccgcga
     7741 gccgttcgat cccgccgcca tcgcggccgc ctacgcccgc gccggcgcac aggccatgtc
     7801 cgtgctgatg gaccgcaggt attttgccgg cggcgaagag gcgtttcgag ccgttcgagc
     7861 ctcggtgtcc ctgcctctgc tctataagga gttcgtggtg gacccctggc aggtctggca
     7921 cgcccgggcc ctcggcgcgt ccgcggtcct tctgatcgcc gcggccctgg acgaccggga
     7981 attggcggcg ctgctcacgg aatgccgggc ggccgggctg gaggtcctgc tcgaggtcca
     8041 tgacgagacc gaaatgcggc gggccgccgc tagcggcgcg gagtgcatag gagtcaacaa
     8101 ccgcgacctg cgcacgttca ctgtagatct cggtaccacc gaacgattga agacactcgc
     8161 gccgacgcgg tgtacactgg tcagcgaaag cgggatccgt accgcggcgg atgtcttgcg
     8221 tctccaagcc gcgggggtgc aggccgtgct ggtgggcgag cacttgttgc ggcagccgga
     8281 tctggagcgg gcggtgcgcg aactgatggg cttggcatgg gagggggaat catgaaaaca
     8341 gtcggtattc tgtccggact cgtggcgttc gtgctgtgcg cggcggcagg cctgtggatc
     8401 ctgacgaagg tgggcttcga tcacggcgac gacgtgatgt ccaccggcat cgggctctac
     8461 ttcatcggca aggccttctt cgtcgggccc atgctgatcc tggccgcgct caagcattga
     8521 acagaaggcg caaagaacgc caaggggcgg gggagaagaa gtccgcgctc ttcccctgaa
     8581 ccctttgacc tatccatgcc ggtcctcgtg aaaatctgcg ggctctgctc ggcggctgac
     8641 gtccgggctg tcgcggcgct gaagccggac gcgctcgggt tcgtcttctg gccgggctcg
     8701 aagcggtgtg tccgggccgc ggacgtcgca acctggacgc gcgatctgcc gccgggcctg
     8761 ctgaaagtgg gcgtgttcgt caatgccgcg ccggcggaac tggaagccac ggtccgaaag
     8821 gccggcctgg acgtggtcca gtaccatgtt ttccaaacat tggaaaaaga atccgcgaat
     8881 tttccagaca ttggaaaaaa tatccggctt ttttccaaac attggaaaac cgtccatctc
     8941 gaccgcggcg ggccgccgcc gatagagtcc gcgttcgttg acgcgttcct ggtggacagt
     9001 tatagtgccg attcgccggg gggcaccggg cggttaggga actgggccgc ggcgcgggcg
     9061 ttcgtggagc gctgcggcaa gcccgtcgtg ctggccgggg gcctgacgcc ggagaacgtg
     9121 gccgaggcga ttcggacggt gcggccgtgg ggcgtggatg tcagttcggg cgtggagatc
     9181 gctcccggcc gcaaggactt gaagaaggtg gaagaattta ttcgggcatg ccgcgaaaac
     9241 gcatagacac ggagccggac gcgcacggcc acttcgggcc gtacggcggg atgttcgtcc
     9301 ccgaaacgct gatggctcct ctccaggatc tcgagcgcga gttccgcgcg gcgcggcgcg
     9361 atccgtcctt ccgccgcgac gtggagtact acctgcgcga gtacgtcggg cgcccgaccc
     9421 cgctctattt tgccgagcgc atgacccggg aactcggcgg gccgaggatt tatctcaagc
     9481 gcgaggacct gtgtcacacc ggcgcgcaca agatcaacaa ctgcatcggc cagtgcctgc
     9541 tcgcgcggcg catgggcaag aagcgtgtca tcgccgagac gggcgcgggc cagcacggcg
     9601 tcgcgacggc gacctgcgcg gcgctgttcg ggatgcagtg cgtcgtctac atgggcaaag
     9661 tggacatgga gcgccaggcg ctcaacgtct tccgcatgcg cagtctcggg gccgaggttg
     9721 tgcctgtgac ggccggccag caaacgctga aggaggctat cagcgaagcc atgcgcgact
     9781 gggtgacgaa cgttcgcacc acgcattacg tcctcggcac ggcctacggc gcgcacccgt
     9841 acccggtgat ggtccggacc ttccagagcg tcatcgggcg cgaggcgcgg cggcagattc
     9901 tcaagcagga aggccgcctc ccgtccgcgc tcgtcgcctg cgtcggcggc ggcagcaacg
     9961 cgatcggact cttttatccc ttcctccagg accgcggcgt ggaaatgatc ggcgtcgagg
    10021 ccggcgggct cggcatccgc agcgggaagc acgccgcccg gttcgccgac gggcgggtgg
    10081 gggtgc
//
LOCUS       DAOOTK010000112        10034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4366_length_10034_cov_4.112035, whole genome shotgun sequence.
ACCESSION   DAOOTK010000112 DAOOTK010000000
VERSION     DAOOTK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4366_length_10034_cov_4.112035"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK634_11290"
     CDS             <1..292
                     /locus_tag="PK634_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOW98360.1"
                     /translation="IPYRIGHFFIAIDISFFTPLEAFRKTTGDILRQLRASKKAPGAE
                     RIYTCGEKEHLAWLERKDKGVPLNGELQKQVTAMRDELKLAQYRFPWEKGRG"
     gene            297..1172
                     /locus_tag="PK634_11295"
     CDS             297..1172
                     /locus_tag="PK634_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOW98361.1"
                     /translation="MAGHLWLLLGIGLFSSVEVASKWVGRGIPPLWLAVARFSLTGLL
                     LMGPAVFALRRREADLTWRDAGRFLVLALIGVTLSIGLYHLAIPRMRANSAAILFSAN
                     PAFVVLFSPWLLGERPGRRKIAGMLCGLAGVALFLSRPAAGLDTRAGIVLMTASLILF
                     ALYSVLAKREMPRFGPLPILSLAGLAGSALLVPLAWWREGPPGPALAHLSWPGFLYLT
                     LCATAIAYVAYFSGLQRLGAARGSFYFFLKPVLASLIAWLWLGERLTPQLVIGAAFVL
                     AGLALTMMPGPRAQR"
     gene            complement(1323..2639)
                     /locus_tag="PK634_11300"
     CDS             complement(1323..2639)
                     /locus_tag="PK634_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98362.1"
                     /translation="MMNHRQIGRLLAVCAAGWAGLARAEGPIEVPLAYQAVGENSREF
                     YPRGMISPELAAAAPEGEWKLPEWTSKHPVFTRIKLGDREHLLVLDVSSPRSKMFDRL
                     YFDANANRDLTDDPVISGKVEAGYGNQDVEFPPMAVTVETGGRSLPYRMRPSIYAYKR
                     GSGETSKAFPRMTIRFMLQTLCYYRGEFEAGGARYRLHLADVNANGVFGEAATEPDSG
                     GGRIFMAGDAFYLSTNKPGYYEAMTCGTHWHLGGQLFAVSLAPGLEKLTLTPAEGPLF
                     PLSFSTEPDRIMLFNAGAKQSVACFRPGREIRLPAGSYRFADYRLLRKETNGALWQLL
                     ASATTGAPPVEVGADRQAELVLGEPFRPVVEIPEWSRAVKSGNQPVRMSFAIRGAANE
                     IVNDVSLLSGQSALTMGKRGARPKEPAYTIATPEGELVQRGSFEYG"
     gene            complement(2636..3397)
                     /locus_tag="PK634_11305"
     CDS             complement(2636..3397)
                     /locus_tag="PK634_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98363.1"
                     /translation="MTACDIGDAGPAEGAPVSFHWSLWWSLGSRLGWFILLPLMLLKE
                     NRRRAAWWIAVPVALETALLSYAGRWLAMLDMMMPLPGIVMALAACWLLGDRLPRRSA
                     WRRAGAALAIWGTACGLAWAAAAESFSGFLVLLELLPALLAGLVFAGACALSLRRGPG
                     IRPFAVRMLPWLAALMAAMALLLALVFMGASWEAGMSEYLLPIAGAAVLWGAAAGFVI
                     WLLLLPFLLLGARSSLYAARLKAMLSPEEKPGEAP"
     gene            complement(3458..4570)
                     /locus_tag="PK634_11310"
     CDS             complement(3458..4570)
                     /locus_tag="PK634_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98364.1"
                     /translation="MKRPAPILIGLAMLSAAPAPAEDVAMAWQESGFQHSLYVDLENQ
                     EVAFKKEPAYAGEQVLRAALPFGAARAEQLPFALDWTGKKLRLDANYNLDLTDDPVLD
                     ADASISDRLIFKGVELEAAGPPARRYRADLEFPPGGSFMLANLRSGWVGTWLRGTSTW
                     RVVFVDDLDGAWDEDDLFAMAADSAEFQKDPRAFALEPVQGLFIDGVHSEIRLAPAAD
                     GTWLFSMRETAVPMGRLRVAGDMVERLVLQGGAKGRKAILNKPGPDPVTVPAGTYPVQ
                     DITLATSIGRLTSSRPADLVVRAGEETELKVGAPLRSRGTAHRDRGEIEVQYELQGVG
                     GEAYRLPADRRTDENRPRVEIFKGGRRVARGAFEFG"
     gene            complement(4567..5349)
                     /locus_tag="PK634_11315"
     CDS             complement(4567..5349)
                     /locus_tag="PK634_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOW98365.1"
                     /translation="MNARQITQTLSLLLLNSSWGPELKWFCAPVLNCHSCSLAWFACP
                     IGVFVHYAGYRVFPFLAAGAVLLLGVLLGRLLCGWICPFGFLQDILHKIPSPKFGLPR
                     WTRGIKYAVLLGMVIAAPFFLGEQTLYSFCRVCPASAVQVTAPNAVRAGFAGLETLTL
                     VKLGVLVAVLALAVFCCRSFCRVFCPLGALLAPLNFLSFWTVKVPTADCKQCGKCDAL
                     CPVEGKPSLRAAEGVDPGRAGECIACHECRSACPRDGGKGDS"
     gene            complement(5395..5655)
                     /locus_tag="PK634_11320"
     CDS             complement(5395..5655)
                     /locus_tag="PK634_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98366.1"
                     /translation="MNRARLEKAAGWTALAAALVLVGIGWFRSHKVYAADSEEYGILV
                     FERITDRQLVEDATFSGVLRREGRLFSTYDRSQVRGKQACPT"
     gene            complement(5652..6008)
                     /gene="folB"
                     /locus_tag="PK634_11325"
     CDS             complement(5652..6008)
                     /gene="folB"
                     /locus_tag="PK634_11325"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOW98367.1"
                     /translation="MDRIYIRDLALRCMIGVFPEERREKQDVVINVVLECDHRPAARS
                     DRLEDAVDYKALKKKIVALVESSQFYLVETLAERIAAACLANPKVEQATVTVDKPGAL
                     RFARSVGVEITRGRAG"
     gene            complement(6040..6762)
                     /locus_tag="PK634_11330"
     CDS             complement(6040..6762)
                     /locus_tag="PK634_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW98368.1"
                     /translation="MELNGKTALVTGGAVRIGAAIVRALARAGADVVIHGHRSVEPAR
                     ALRREVEALGRRAFCVRGDLRREAEVRRVVRESREKGGRLDILVNNAAVFHKDTLRSL
                     TARKLREEFDVNLFAPLLLMRAFAEESGRGSIVNLLDRRITSLDRECLPYVLAKKALA
                     EATRIAALDLAPKIRVNAVAPGAILPPPGEGMAYLKDKAGPIPLRRRCAPEDVAEAVL
                     LLLKNDSLTGQIIFVDGGQHLL"
     gene            complement(6782..7711)
                     /locus_tag="PK634_11335"
     CDS             complement(6782..7711)
                     /locus_tag="PK634_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOW98369.1"
                     /translation="MAGPSPERRRRALHYALVVPLRVALLVLAGLVIMFAAFQRRYIY
                     YPMRGDPAAFAAAAGRAGLAAWSDARGRTIGWRTRRSLLAPPQCRAVVFHGNAGFALH
                     RLYFVDGLSAAGEGLDWEVLLFEYPGYGAREGTPSESAIQAAAEEAVQSLLAENDKPL
                     FLVGESLGCGVAAHLAARFPARIAGLFLVTPFTRLADIAAHHYPFLPVRWLLREKYDA
                     ASDLARFAGPVAVLLAGRDEVIPPRLGRALFDGYAGPKRLWVQEEAGHNSLDYEPGAG
                     WWREVANFLVESVAPPALPDAAAGTTNSFTGVK"
     gene            complement(7716..8813)
                     /locus_tag="PK634_11340"
     CDS             complement(7716..8813)
                     /locus_tag="PK634_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOW98370.1"
                     /translation="MTAVRCPVNEAAFISRDEPALILDDRKITYSEFEFYVAGAAARL
                     RKAGCRAGDRVALGVREPWQAAVLLMALLRVKAVACLLAGEEDGAGKAARAECGIRIG
                     GPAPGLDAESLLPVCIEGQDSAEASSLALEQSATVLFLADRDGGLRAVLHTYGNHYYG
                     ARGANHAVRLSSHGRWLSAPPLYTGAGLSAFFRCLLSGAALVLPEPGCPLRESLARHE
                     VTHASVTPGDLEDIAERRASADFPKLRAVLVESDEVQPALLRKARAAGWPVFPGYGIP
                     EMASGISTVMPDSPPPRRDGAGLPMKYREVRVSEAGEILVRGPALFAGYVEPGVLRRP
                     VDGDGWFSTGARGRMESDGYLTVAAPDAKAG"
     gene            complement(8930..>10034)
                     /locus_tag="PK634_11345"
     CDS             complement(8930..>10034)
                     /locus_tag="PK634_11345"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOW98371.1"
                     /translation="LLRSFGAAGTEETGYLVVPNGSGSLIRFNNGKSGTAAYSQYIYG
                     MDLIDADYTKTQNTQAARMPLYGICRENSSVLVTIERGAPLCYLTADVSGRYNSYKAV
                     IQSLTFNPNLALKLNEQVSVAAGVSAMWIDLDLRRKLPNPLVAGGPDLDFKLCGDSIG
                     YGFNVAGRWEPLEWLSFGAAYQGQVDQDIDGDATVSGRSGDASGDVSLPDMVFLGTAV
                     KPMKKVTVSLDAVRTGWSSYDQFALDLDPTLLGPDKAYQVSQKDWNDVWRYELGVEYA
                     LNEAWTLRAGYAFDEEPGPDSTADYLVPANDRNLYCLGAGYQWKAWTFDVSYTYLEIE
                     DRTIAARPEHGVLPSEFECGKSHLFGLTVGRKI"
BASE COUNT         1657 a         3534 c         3361 g         1482 t
ORIGIN      
        1 aatcccctac cggatcggac acttcttcat cgcgatagac atctcgttct tcacgccgct
       61 cgaggccttc cggaagacca cgggcgacat cctgcggcaa ctgcgcgcgt cgaaaaaggc
      121 gccgggcgcc gagcggatct acacgtgcgg cgagaaggag cacctggcct ggctggagcg
      181 caaggacaag ggcgtgccgc tgaacggcga actgcagaag caggtgacgg ccatgcggga
      241 cgaactgaag ctcgcacagt accgcttccc gtgggaaaag ggccggggat aggaccatgg
      301 cgggccacct ctggctgctc ctgggcatcg ggctcttcag ctcggtggaa gtcgcgagca
      361 agtgggtcgg ccgcggcatc ccgccgctct ggctcgccgt ggcgcgcttt tccctgaccg
      421 ggctgctcct gatggggccc gcggtcttcg cgctgcgccg cagggaggcg gacctgacct
      481 ggcgcgacgc cggccgcttc ctcgtcctgg cgctgatcgg cgtgacgctg tccatcgggc
      541 tctaccacct ggcgatcccg cggatgcgcg cgaattccgc ggcgatcctg ttcagcgcga
      601 acccggcgtt cgtggtgctg ttctccccgt ggctactcgg ggagcggccg ggacggcgca
      661 agatcgcggg catgctctgc gggttggcgg gcgtcgcgct gttcctgtcc cggccggcgg
      721 cggggctcga cacgcgcgcg ggtattgtcc tgatgacggc ctcgctgatc ctcttcgcgc
      781 tctactccgt cctcgccaag agggaaatgc cgcggttcgg cccgctgccg atcttgagcc
      841 tggccgggct cgcgggcagc gcactgctgg ttcccctggc ctggtggagg gaaggcccgc
      901 cggggccggc gctggcccac ctatcgtggc cggggttcct gtacctgacg ctgtgcgcca
      961 cggccatcgc ctacgtcgcc tacttcagcg gcctgcaacg gctgggcgcg gcccgcggct
     1021 cgttctattt cttcctgaag ccggtgctgg cctcgctgat cgcgtggctg tggctgggcg
     1081 aaaggctcac gccgcaactg gtcatcggcg ccgccttcgt gctcgccggc ctggcgttga
     1141 ccatgatgcc ggggccgagg gcccagcgct gatcaacggc gcgggtggaa ccgcgccctc
     1201 ccctacggcg tcaactcctg cgtctccagc tgggtcttga acggcccgac ctggtacttg
     1261 cagaagaggt agtaccgctg tcctctttta gggccactcc atgtggccga gcagctgaag
     1321 cctcacccat actcgaagga gccgcgctgg acgagctcgc cttccggcgt ggcaatggta
     1381 taggcaggct ccttcggccg cgcgccccgc ttacccatcg tcagggccga ctggccggac
     1441 agcagcgaca cgtcgttcac gatctcgttc gcggcgccgc gaatcgcgaa gctcatgcgc
     1501 accggctggt tgcccgactt gaccgcgcgc gaccattcgg ggatctcgac cacgggtcgg
     1561 aaaggctcgc cgaggaccaa ctcggcttgg cggtcggcgc cgacctcgac gggcggcgcg
     1621 ccggtcgtgg cggaggccag caactgccag agcgcgccgt tggtctcctt ccgcagcaag
     1681 cggtagtcgg caaaccggta acttcccgcc gggagccgga tttcgcggcc cggccggaaa
     1741 cacgccacgg actgcttggc gccggcatta aagagcatga tccggtccgg ctcggtgctg
     1801 aacgacaggg ggaacagcgg cccctcggcg ggggtcaggg tcagcttctc gaggcccggg
     1861 gccagcgaca cggcgaaaag ctggccgccc aggtgccagt gagtcccgca ggtcatcgct
     1921 tcgtagtagc cgggcttgtt ggtggacagg tagaacgcgt cgcccgccat gaagatccgc
     1981 ccgcccccgg aatccggctc ggtcgccgcc tcgccgaata cgccgttggc gttgacatcg
     2041 gccagatgca gccggtatcg cgccccgccg gcctcgaact cgcccctgta gtaacagagg
     2101 gtctgcagca tgaagcgaat cgtcatcctg gggaaggcct tgctggtctc gcccgacccc
     2161 cgcttgtagg cgtagatgga cggcctcatc cggtacggga gggaacggcc cccggtttcg
     2221 acggtcacgg ccatcggtgg aaactcgaca tcctggttcc cgtagccggc ttccactttg
     2281 ccggagatca ccggatcgtc cgtgagatcc cggttcgcgt tggcgtcgaa gtagaggcga
     2341 tcgaacatct tgctccgggg ggaagagaca tcgaggacca gcaggtgctc ccggtcgccc
     2401 agcttgatcc gcgtgaagac cggatgcttg ctcgtccact ccggcagctt ccactcccct
     2461 tccggcgcgg cggcggccag ctcgggggat atcatgcctc ggggatagaa ctcgcgggaa
     2521 ttctcgccca cggcctggta cgcgagcggc acctcgatcg ggccttccgc ccgcgccagg
     2581 ccggcccatc ccgcggcgca aaccgcgagc agccgcccga tctgccgatg gttcatcatg
     2641 gggcctctcc gggtttttct tccggggaca gcattgcttt tagtcgtgca gcatacaatg
     2701 aactgcgcgc gccaagcagc aaaaaaggca acagcagcag ccagatcaca aatcccgcgg
     2761 ccgctcccca caagacggcg gcgccggcga tgggcagcag atactccgac atcccggcct
     2821 cccacgaggc ccccatgaat acgagcgcca gcaggagcgc catcgccgcc atcagggccg
     2881 ccagccacgg aagcattcgg accgcgaacg gcctgatccc gggcccgcgg cgcagggaca
     2941 gggcgcacgc gccggcgaag accaggccgg cgagcagcgc gggcagcagt tccagcagaa
     3001 caagaaaacc cgaaaagctc tcggccgccg ccgcccaggc caggccgcaa gccgtccccc
     3061 atatcgccag cgccgccccg gcccggcgcc acgcggagcg gcggggcaga cgatcgccca
     3121 ggagccagca tgccgccagc gccataacga tgcccggcag gggcatcatc atgtccagca
     3181 tggccagcca ccggcccgcg tacgacagca gcgcggtctc gagggccacg ggcacggcga
     3241 tccaccacgc cgcgcggcgg cggttctcct tcagcagcat caagggcagg aggatgaacc
     3301 agccgagccg cgagccgagg ctccaccaga gcgaccagtg gaaggagacg ggcgcgcctt
     3361 ccgccgggcc ggcgtcaccg atatcgcacg ccgtcacgat ccgcaggtcg ccgaagacgt
     3421 tgagaggtac tcgccatgag tacgcgcagg agcctcctca cccaaactcg aacgcgccgc
     3481 gggccaccct tcgaccgcct ttaaaaatct cgacgcgcgg ccggttttcg tcggtgcgcc
     3541 ggtcggccgg cagccggtag gcctccccgc cgacgccttg gagctcgtac tgaacctcga
     3601 tctcgccacg gtcgcggtgc gccgtgccgc ggctccgcaa cggagccccg accttgagtt
     3661 cggtttcttc gcccgccctg accaccaggt ccgccggccg gctcgaggtc agacgcccga
     3721 tgctggttgc cagcgtgatg tcctgaacgg gataggtccc ggccggaacc gtcacgggat
     3781 cggggcccgg cttgttcagg atggccttcc gccccttcgc gcctccctgc agaacaaggc
     3841 gctccaccat gtcgccggcc acgcgcagcc gtcccatcgg cacggcggtc tcccgcatcg
     3901 agaacaacca cgtcccgtct gcggcgggag ccagccggat ctcgctgtgc actccgtcga
     3961 tgaacaggcc ctgcacgggt tcgagggcga aggcgcgggg atctttctgg aattccgccg
     4021 agtcggccgc catggcgaac aggtcgtcct cgtcccacgc gccgtcgaga tcgtcgacga
     4081 agaccacgcg ccacgtcgag gtgccgcgca accacgtgcc gacccatccc gagcggagat
     4141 tggccagcat gaaggagccg cccggcggga attccaggtc ggcccggtag cgccgcgcag
     4201 gaggcccggc cgcctccaac tccacgccct tgaagatcag ccggtccgag atggacgcat
     4261 cggcgtcgag aaccggatca tcggtcagat ccaggttgta attggcatcc agacgcagtt
     4321 ttttcccggt ccagtccagg gcaaagggaa gctgttcggc ccgcgcggcg ccgaacggca
     4381 gggcggcgcg caagacctgc tccccggcat aggcgggctc cttcttgaag gccacttcct
     4441 gattctcgag atcaacatag aggctgtgct ggaatccgct ttcctgccag gccatggcca
     4501 cgtcctcggc tggcgccggc gcggccgaca gcatggcgag cccgatcagg atcggcgccg
     4561 ggcgtttcat gaatctccct ttccgccgtc gcgcggacag gcggaccggc actcgtggca
     4621 ggcgatgcat tcccccgcgc gcccgggatc cacgccctcg gcggcgcgga gcgacggctt
     4681 cccctccacg gggcagagcg cgtcgcattt tccgcactgc ttgcagtccg ccgtcgggac
     4741 tttgaccgtc cagaaggaaa gaaagttcaa cggcgccagc agcgcgccca gcgggcagaa
     4801 cacccggcag aacgaccggc agcagaagac ggccagcgcg agcacggcca cgagaacgcc
     4861 cagcttgacc agcgtgaggg tctccaagcc ggcgaacccc gcccgcaccg cgttcggcgc
     4921 cgtcacctga accgccgacg ccgggcagac ccggcagaac gagtacagcg tctgttcgcc
     4981 caggaagaag ggcgccgcga tcaccatgcc cagcaggacg gcgtacttga tcccgcgggt
     5041 ccagcgcggc agcccgaact tggggctggg gatcttgtgc aggatatcct ggagaaagcc
     5101 gaaagggcat atccagccgc acagcagccg gcccagcagg acgccgagga gcagcacggc
     5161 gcccgcggcc agaaacggga agacgcggta tccggcgtag tgcacgaaca ccccgatcgg
     5221 gcaggcgaac caggccagcg agcaggagtg gcagttgagc acgggggcgc agaaccactt
     5281 cagctccggg ccccaggaac tgttcaacag cagcagcgaa agagtttgag ttatttgtcg
     5341 cgcgttcacg atgaagaact ggaagcggcc gccatggccg cgggtggcct actttcaggt
     5401 agggcacgcc tgcttgcccc ggacctggga gcggtcatag gtggaaaaca gccgcccctc
     5461 ccgccggagc acgccgctga acgtcgcgtc ctcgaccagt tgccggtcgg tgatgcgttc
     5521 gaacaccagg atgccatact cctcggaatc ggccgcgtag accttgtgcg agcggaacca
     5581 cccgatgccg acgaggacca acgccgcggc cagcgccgtc caacccgccg ctttctccag
     5641 ccgcgcccgg ttcatccggc ccgcccccgg gtgatctcca cgcccacgct ccgcgcgaac
     5701 cgcagggccc ccggcttgtc caccgtcacc gtcgcctgct ccaccttcgg gttcgcgagg
     5761 caggcggcgg cgatgcgctc ggccaacgtc tcgaccaggt agaactggct gctttcgacc
     5821 agcgccacga tcttcttctt gagcgccttg tagtccaccg cgtcctccaa ccggtcgctg
     5881 cgggcggcgg gccggtgatc gcactccagg acgacgttga tcaccacgtc ctgcttctcg
     5941 cgccgctcct cggggaatac cccgatcata caccggaggg cgaggtcgcg tatgtagatg
     6001 cggtccatga ggatactgta ggattcagcg gctccgcagt caaagcaagt gctgcccgcc
     6061 gtccacaaag ataatctggc cggtcagaga atcatttttc agcaacaaaa gaacggcctc
     6121 ggccacgtcc tcgggcgcgc accggcggcg gagcggaatc ggcccggcct tatccttcaa
     6181 gtaggccatc ccctcccccg gcggcgggag gatcgcgccg ggcgccacgg cgttgacgcg
     6241 gattttcggc gcgaggtcga gcgcggcgat ccgcgtggcc tcggccaggg ccttcttggc
     6301 caggacatac ggcaggcact cgcgatcgag gctcgtaatc cgccggtcga ggaggttcac
     6361 gatgctcccc ctgccgcttt cctccgcgaa ggcgcgcatc aggagcagcg gcgcgaagag
     6421 gttgacgtcg aactcctcgc gcagtttccg cgccgtgagg gaccgcagcg tgtccttgtg
     6481 aaacacggcg gcattgttga cgaggatatc gagtcgtccg cccttttccc tcgattcgcg
     6541 gaccacgcgc cggacctccg cctcgcgccg caggtcgccg cggacgcaga aggccctccg
     6601 cccgagcgcc tccacctccc gccgcagcgc ccgggccggc tcgacggacc ggtggccgtg
     6661 gatgacgaca tccgctcctg cccgggccag ggcgcggacg atcgcggcgc cgatccgcac
     6721 ggccccgccg gtcaccagcg ctgtcttccc gttcagctcc atgccgaatc actgtaacag
     6781 ctcacttcac acccgtgaat gaatttgtgg tgcccgccgc ggcgtccggc agagccgggg
     6841 gggcgacgga ttccaccagg aagttcgcca cctcccgcca ccatccggcc cccggctcgt
     6901 agtccagcga gttgtggccc gcctcctcct ggacccacag gcgcttcggc ccggcgtacc
     6961 cgtcgaacag cgcgcgcccg aggcggggcg ggatcacctc gtcgcgcccc gccaggagca
     7021 cggccaccgg ccccgcgaag cgggccaggt cggacgcggc atcgtacttt tcgcgcagca
     7081 gccagcggac ggggaggaac gggtaatggt gcgccgcgat gtccgcgaga cgggtaaagg
     7141 gcgtgaccag gaacaggccg gcgatccggg ccgggaaccg cgcggcgagg tgcgcggcca
     7201 cgccgcagcc cagcgactcg ccgacgagga acagcggctt gtcgttctcg gcgaggagcg
     7261 attgaacggc ctcctccgcc gcggcctgga tggcggactc cgagggggtt ccttcgcgcg
     7321 ccccgtagcc gggatactcg aagagcagga cctcccagtc caggccctcg cccgcagccg
     7381 agagcccgtc cacgaaatac aggcggtgca gcgcgaatcc ggcgttgccg tgaaagacca
     7441 cggcccggca ttggggcggc gccagcaggg accgccgcgt ccgccagccg atggtccggc
     7501 cacgggcatc cgaccaggcc gcgaggcccg cccggcccgc cgcggccgcg aacgcggccg
     7561 gatccccgcg catgggatag tagatgtacc ttcgctggaa cgcggcgaac atgatcacca
     7621 gcccggccag gaccagcaac gcgacgcgaa gcggaacgac aagcgcgtag tgcagcgcgc
     7681 ggcggcggcg ttcgggggat ggaccggcca tcgtttcatc ccgccttcgc gtccggcgcc
     7741 gccaccgtga gataaccgtc ggattccatc cgtccgcgag ctcccgtgct aaaccagccg
     7801 tcgccgtcca ccgggcgccg aaggactccg ggctcgacgt aaccggcgaa cagcgccggg
     7861 ccgcgaacca ggatctcgcc ggcctcgctg acgcgcactt cgcggtattt catcgggagc
     7921 ccggcgccgt cccggcgagg gggcgggctg tccggcatca cggtgctgat cccggaagcc
     7981 atctcgggga tcccgtatcc cgggaacacg ggccagccgg cggcccgcgc cttccgcagc
     8041 agggccggct ggacttcgtc gctttcgacg agcacggccc ggagcttcgg gaaatccgcc
     8101 gaagcccgcc tttccgcgat atcctccagg tcgccgggcg tcacgctggc gtgcgtgacc
     8161 tcgtgccggg ccaggctctc gcgcaacgga caaccgggct cgggcaggac cagcgcggcc
     8221 ccgctcaaga ggcagcggaa gaaggcgctt aggcccgcgc ccgtgtacag cggcggcgcg
     8281 gaaagccagc ggccgtgcga cgacaggcgg acggcatggt tggcgccgcg cgccccgtag
     8341 tagtgattgc cgtaggtgtg caggaccgcc cggagccccc cgtctcgatc cgccaggaac
     8401 aggacggtcg ccgactgctc caaggccagg ctggaggctt cggccgagtc ctggccctcg
     8461 atgcagaccg gcagcaggga ctccgcgtcc aggccgggcg ccggcccgcc gatccggatc
     8521 ccgcactccg cccgggcggc cttcccggcc ccgtcctcct cgccggccag caggcaggct
     8581 accgccttca cgcgcaagag ggccatgagc agaacggccg cctgccaggg ctcccgcacg
     8641 cccagggcga cgcggtcccc cgcccggcac ccggccttcc gcaggcgggc cgcggcgccc
     8701 gccacgtaga actcgaactc ggaataggtg atcttccggt cgtcgagaat cagcgccggc
     8761 tcgtcgcgcg aaatgaacgc cgcctcgttg acagggcatc gcacggcggt catcgggtcc
     8821 actctccgtt cggcatgatg tcatgctacc cgaaaccggc gggaaaaacc accggggaag
     8881 aaaaaagccc ggggctcggg ccccgggctt gtccgcgatc gggcgcggat cagatcttgc
     8941 ggccgacggt caggccgaac aggtgcgact tgccgcactc gaattccgag gggagaacgc
     9001 cgtgttccgg ccgggcggcg atggtgcggt cctcgatctc gaggtaggtg tacgagacat
     9061 cgaacgtcca cgccttccac tggtagcccg cgccgaggca gtacaggttg cggtcgttcg
     9121 cggggaccag gtaatccgcc gtgctgtccg ggccgggctc ctcgtcgaac gcgtagccgg
     9181 cgcgcagggt ccaagcctcg ttcagcgcgt actcgacgcc gagctcataa cgccagacat
     9241 cgttccagtc cttctgcgag acctggtagg ccttgtccgg ccccagcagc gtgggatcga
     9301 ggtccagggc gaactggtcg taggaactcc atcccgtgcg cacggcgtcg agggacacgg
     9361 tcactttctt catgggcttc acggcggtgc ccaggaagac catgtccggc aggctgacgt
     9421 cgcccgaggc gtcgccgctg cggccgctga ccgtggcgtc gccgtcgatg tcctggtcca
     9481 cctggccctg gtaggccgcg ccgaaggaaa gccactcgag cggctcccag cggccggcga
     9541 cgttgaagcc gtagccgatc gaatcgccgc agagcttgaa gtccaggtcc ggcccgccgg
     9601 ccacgagggg gttcgggagc ttgcggcgca ggtcgaggtc gatccacatg gccgacacgc
     9661 cggcggccac ggaaacctgc tcgttgagct tgagcgccag attgggattg aaggtgaggg
     9721 actggatgac ggccttgtag ctgttgtagc ggccggagac gtccgcggtt aaatagcaca
     9781 ggggcgcacc gcgctcgatg gttacaagca cgctgctgtt ttcacggcag atgccataca
     9841 gcggcatgcg cgcagcttgc gtgttctgcg tcttggtata atccgcgtcg atcaggtcca
     9901 tgccgtaaat atactgggaa taggcggctg tgccgctctt gccgttgttg aagcggatga
     9961 gcgaaccgga gccgttgggc accaccaggt accccgtttc ctccgtgccg gccgcgccaa
    10021 aggagcgcag aagc
//
LOCUS       DAOOTK010000113         9842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4464_length_9842_cov_4.610504, whole genome shotgun sequence.
ACCESSION   DAOOTK010000113 DAOOTK010000000
VERSION     DAOOTK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_9842_cov_4.610504"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PK634_11350"
     CDS             <1..657
                     /locus_tag="PK634_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOW98372.1"
                     /translation="LYFAYPAQISGDKVWAGVADWAHVADGFTGATSLAAAKAAPAGA
                     NAMEFLKESGFTLQRMIVGLEPGSIGETSAIAVALGLLVLVLTGIGSWRIIAGGLLGL
                     AVTGTLLNLLPEATRLANPVLQLPFYFDLVMGGVFFGIVFMATDPVSAAATPAGKWIY
                     GFLIGLMTVVIRAFNPAYPAGNMLAILFCNVTAPLLDHIVLQAHIRGRQRYLARMNHG
                     "
     gene            650..1378
                     /gene="nqrC"
                     /locus_tag="PK634_11355"
     CDS             650..1378
                     /gene="nqrC"
                     /locus_tag="PK634_11355"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOW98373.1"
                     /translation="MAKGDAYTLGYAAAVCVACSLLLSGTAALLKSKQDFQVELDRKV
                     NVLKAFGVATIDGQGRAIGGEEVEKIFGEHISEVVLDGETGQVLPGLAVRDVSRDDLE
                     GKKKLPLFLWKDGDRVTRYAFPISGKGLWSTIYGYLALGEDLSTILGVTFYRHGETPG
                     LGAEISTDAFQKQFVGKKVFKDGRLQPIEVVKGRVANRYPEGSDRAVDGISGATLTGN
                     GINRFLNGDLEKYETYFAGIRGGG"
     gene            1375..2004
                     /locus_tag="PK634_11360"
     CDS             1375..2004
                     /locus_tag="PK634_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOW98374.1"
                     /translation="MSGMAGNLKKVLVDPLSENNPVTIQILGICSSLAVTVKLETALV
                     MAIGLTVVTAGSNWVISLLRNTIPSKIRMIVEMAVVSVLVIVADQFLRAYLFEISRQL
                     SVFVGLIITNCIVMGRLEAYALQNPPGLSFLDGIGNGLGYSLVLVIVASVREILGSGK
                     WLGFRVIPEAAYSAGYINNGFMLLAPGAFIVLGLLIWVQRTLSKKFETD"
     gene            2013..2627
                     /gene="nqrE"
                     /locus_tag="PK634_11365"
     CDS             2013..2627
                     /gene="nqrE"
                     /locus_tag="PK634_11365"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009505962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOW98375.1"
                     /translation="MELISLAIRSIFIENILLSTFLGMCSFLAISKRIDTAMGLGAAV
                     IFVQFITVPANWAIKHFFLEPGALAWTGVPALAALDLSFLIFICFIAVIACLVQFVEM
                     VLDRFFPPLYQSLGIFLPLITVNCAILGGSLFMVERNYTFVQSVVYGFSSGAGWALAI
                     TCMGAIRKKLRYSNIPPGLKGLGITMLLTGLMAMAFMCFSGISL"
     gene            2637..3863
                     /gene="nqrF"
                     /locus_tag="PK634_11370"
     CDS             2637..3863
                     /gene="nqrF"
                     /locus_tag="PK634_11370"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOW98376.1"
                     /translation="MEHPVLYILASVAVFTGIILFLTAGLMIASRRLAPSGPVTIDIN
                     EGYKKLTVDAGATLLSSLASERIFLPSACGGGGTCAMCKCTVTDGGGSILPTETGHIS
                     KQQAKEGLRLACQLKVKRDLKIRIPSDVLEIRKFEGTVRSNRNVATFIKELVIDLPAG
                     TDLKYRAGGYIQIDIPPYELAYRDFDIDERFRDAWDAFRLWDLKVKSAEPIFRAYSMA
                     SYPAEGNLVKLNVRIATPPPGMNAPPGIASSYIFGLKPSDKVVVSGPYGEFFARESDR
                     EMLYIGGGAGMAPLRSHIMDLLVTKKSNRKISFWYGARSLREMFYEEDFRKLEREHPN
                     FKFHIALSEPRPEDDWKGPVGFIHSVVHDRYLANHEDPSEIEYYMCGPPPMIAAVNAM
                     LYNVGVDKDMIAYDEF"
     gene            3888..4949
                     /locus_tag="PK634_11375"
     CDS             3888..4949
                     /locus_tag="PK634_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOW98377.1"
                     /translation="MNPWKLLWVLAVILAFAAFTWRRDPPGPKNPSWAGETMGTLYSI
                     KLADSPLGQGALRELQAEVDRCLVEVNREMSHYQPDSELSQFNRHESAEPFPVSPGFA
                     SVVRFALELNRRSGGVFDPTLGPLIDLWGFGAPGHVSEPPSPAQVEEARARIGGQHLS
                     VTPAGQLVKGIPSLRLNLGAVAKGYGVDEVARVIRANGISNLFVEIGGEVVTAGHNAE
                     GQAWRIGVDAPRPDALPGQELETILHLSGLAVATSGDYRNYFVDGAGMRFTHILDPRT
                     GRPIQHNLASVSVVAPDCMTADGLATTLFVMGAEEGMKWIGSWPGVEALFILREPDGR
                     FVCRPTPGFVALTGCALPE"
     gene            5214..6221
                     /gene="sppA"
                     /locus_tag="PK634_11380"
     CDS             5214..6221
                     /gene="sppA"
                     /locus_tag="PK634_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOW98378.1"
                     /translation="MDKTHSGSHVGFWAVILLLLIALVGSLAMNAGLLLGRALTGWSP
                     EEEEAEDEFPAFDEVWSWGEGEAKVVRIPIQGELFHYVSEGSLFSPSFDPIESILRQI
                     RAAQNDEDVVALILEVSSPGGDMTSADEIYQAVQKFKASGEDRRVIAHVQDLAASGGY
                     YAILPADRIVAEPTAMLGSIGVIMQTLNWKTLGEKLGIADTTIKSGEHKDLLNPFREV
                     SEEQRAILQKIVDSAYSRFFDLVVTNRGVEPAKLRELADGRVFDASVALEGGLIDDIG
                     YWTDVLDQLPEVTGEDSVKIVRYSHSLTFSDWLGSVHARFRWPRWIQSDAPRLMYLWR
                     P"
     gene            6324..7418
                     /locus_tag="PK634_11385"
     CDS             6324..7418
                     /locus_tag="PK634_11385"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOW98379.1"
                     /translation="MLYRLIVLSGPLKGRRLTVERAPTTVGRDPACSIHLDDEEVALR
                     HAVFEHNDTGLVVRDLGTMNRVLVNKREVREAHLRHGDEIEIGRTRFLVQALLQTEVE
                     APAEEEGEPRRGLGATPRVLLLLLLFLALFIAYQRWAAQRTRPTTAFRPPVETPAVAP
                     SNVPAPIEPPALPPQMEEELRSMRATLATIKETVEDLGTRTAMPPAAPVSPAVPSEPP
                     VPSPDYTAWLAIESVEQVKLPQDDSPDEARLLRLRLRAAPGVTIEPDGVRMEVQLFIR
                     EEETLRIFPARVEVLVSPPEFHDGVLTNGGSAIATMQCRVPGAAGRLGRYYGFRLKLF
                     YKDVLQAEVDRPRTLAADLWRETDGELSPD"
     gene            7425..8204
                     /locus_tag="PK634_11390"
     CDS             7425..8204
                     /locus_tag="PK634_11390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW98380.1"
                     /translation="MREKISACVICFNEERKIRRCLASLSWCDEIIVMDSFSTDQTPA
                     ICREYTERFYQHEWLGYIAQRNMVRDLALYPWILYLDSDEEVSPALRDEILAEFERGT
                     DPFVGYEFPRQVYYIGRWIRHGEWYPDVKLRLFRKSRGRTEGIEPHDTVVVKGPVQRL
                     QAPVWHYTYDDLRDHMDTANRFSTISAQQKFVQEVPFRWLDLFLRPPFHFLKGYVLRA
                     GFLDGMHGFIIAVISAYAAFAKYAKLWELERRHKHHFRELP"
     gene            complement(8212..8709)
                     /locus_tag="PK634_11395"
     CDS             complement(8212..8709)
                     /locus_tag="PK634_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOW98381.1"
                     /translation="MKRYSHFSEVDWAHWNPTERATLMFVIRDGQVLLIHKKKGLGAG
                     KINGPGGRLDPGETPRQAAIREVQEELVVTPTGVREAGELMFQFVDGFSIHGYVFTAT
                     GIEGEPQETGEAIPLWTPLDQIPFDRMWEDDRVWIPLMLAGTPFHGRFLFDGDKMLEY
                     RLEKG"
     gene            complement(8755..>9842)
                     /locus_tag="PK634_11400"
     CDS             complement(8755..>9842)
                     /locus_tag="PK634_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98382.1"
                     /translation="LGWIFSAASRHRPVLASVFFAALLLPLVPLVRQQRDRELVRVYG
                     GSSQSGHDFGWQFGRHQLEGLGGIVADLQPGETPLPARDYPPPLERGALLFGGTDPGR
                     FVPTYMIFSAGIRPDVFLLTQNALADRTYLNALRDLYGGAIRIPSPADADAAFYQYME
                     ELQAGRLPPGTGAKPQGDRVTVQGVAGVMAINGILARRFFEANKDRHAFYIEESYVIP
                     WMFPHLAPHGLIMRLHAEPLPSLPPELVNQDRAFWNWYSRRLLDRPAFLRDVAARRSF
                     CKLRCAIAGLYAYRGLFDEAEYAFRQALALYPDSPEANYRLADVLMQRRKFNDAEAVI
                     AACLRADPGNDKVRSFLELIRRAAAGQ"
BASE COUNT         1623 a         3224 c         3295 g         1700 t
ORIGIN      
        1 ctctactttg catacccggc gcagatctcg ggcgacaagg tctgggccgg cgtggccgac
       61 tgggcgcacg tggccgacgg gttcacgggc gcgacttcgc tggccgcggc caaggcggcg
      121 ccggccggcg cgaacgcgat ggagttcctc aaggaatccg gcttcacgct ccagcgcatg
      181 atcgtcggcc tggagcccgg cagcatcggc gagacgtccg cgatcgcggt ggcgctcggc
      241 ctgctggtcc tcgtgctgac gggcatcggc tcctggcgga tcatcgcggg cggcctgctg
      301 ggcctggcgg tgacgggcac gctgctcaac ctcctccccg aggcgactcg cctggcgaac
      361 ccggtgctcc agttgccctt ctacttcgac ttggtcatgg gcggcgtttt cttcggcatc
      421 gtgttcatgg ccaccgaccc ggtttccgcg gcggctacgc ccgcgggcaa gtggatctac
      481 gggttcctga tcggcctgat gaccgtcgtc atccgcgcgt tcaatcccgc gtacccggcg
      541 gggaacatgc tggccatcct gttctgcaac gtgacggccc cgctgctgga ccatatcgtg
      601 ctgcaggcgc acatccgcgg acggcaacgc tacctggcga ggatgaacca tggctaaggg
      661 cgacgcctat acgctgggct acgcggcggc cgtttgcgtc gcgtgcagcc tgctgctgtc
      721 gggcacggcc gccctcctga agtcgaagca ggacttccag gtggaactgg atcgcaaggt
      781 caacgtgctc aaggcgttcg gcgtcgccac gatcgacggg caggggcggg ccatcggcgg
      841 cgaggaggtc gagaagatct tcggcgagca catctcggag gtcgtgctcg acggggagac
      901 ggggcaggtc ctgcccggcc tcgcggtccg cgacgtgtcc cgggacgacc tggagggcaa
      961 gaagaagctg cccctgttcc tctggaagga cggggaccgc gtgacgcgct atgccttccc
     1021 gatttcgggg aagggcctgt ggtccacgat ctacgggtac ctcgccctcg gcgaggactt
     1081 gagcacgatc ctcggcgtga ccttctaccg ccacggcgag acgcccggcc tcggcgcgga
     1141 gatctccacc gacgccttcc agaagcagtt cgtcggcaag aaggttttca aggacgggcg
     1201 gctgcagccg atcgaggtgg tcaaggggcg cgtggcgaac cgttatccgg agggaagcga
     1261 ccgcgccgtg gacggcatca gcggcgcgac gctgacgggc aacgggatca accgtttcct
     1321 gaacggggac cttgagaagt acgagacata ctttgccggc attcgcggcg gaggatgagc
     1381 ggcatggcgg gcaaccttaa aaaggtgctg gtggatccgt tgtcggagaa caacccggtg
     1441 acgatacaga tcctcggcat ctgctcgtcg ctggccgtca ccgtcaagct ggagaccgcg
     1501 ctggtcatgg cgatcgggct gacggtggtc accgccggct cgaactgggt catctcgctg
     1561 ctgcgcaaca cgatcccgag caagatccgg atgatcgtgg agatggccgt ggtgtcggtg
     1621 ctggtgatcg tggcggacca gttcctgcgg gcctacctgt tcgagatcag ccggcagttg
     1681 agcgtgttcg tgggcctgat catcaccaac tgcatcgtca tggggcggct ggaggcctat
     1741 gctcttcaga atccgccggg gttgtcgttc ctcgacggga tcggcaacgg attgggctac
     1801 agcctcgtgc tggtcatcgt cgcgtcggtg cgcgagatcc tcggctcggg gaagtggctc
     1861 ggcttccggg tcatcccgga ggcggcctac agcgcggggt acatcaacaa cgggttcatg
     1921 ctgctggccc cgggcgcgtt catcgtgctg gggctgctga tctgggtgca gcggacgctg
     1981 tcgaagaagt ttgaaacgga ctaagggcga acatggaact gatcagcctg gcgatacggt
     2041 cgatcttcat cgagaacatc ctgctctcga cgttcctcgg gatgtgctcg ttcctggcca
     2101 tctcgaagcg gatcgacacg gccatgggcc tgggcgcggc ggtgatcttc gtccagttca
     2161 tcaccgtgcc ggcgaactgg gcgatcaagc atttcttcct ggagcccggc gccctggcct
     2221 ggacgggggt cccggccctg gcggcgctgg acctatcgtt cctgattttc atctgcttca
     2281 tcgccgtcat cgcctgcctc gtgcagttcg tcgagatggt cctggaccgg ttcttcccgc
     2341 cgctctacca gtcgctcggc atcttcctgc cgctgatcac ggtcaactgc gcgatcctcg
     2401 gcggctcgct gttcatggtc gagcgcaact acaccttcgt gcagtccgtc gtgtacggct
     2461 tctcgtccgg cgccggctgg gcgctggcca tcacctgcat gggcgcgatc cgcaagaagc
     2521 tgcgctactc caacatcccg ccgggcctga agggcctcgg gatcacgatg ctgctgacgg
     2581 gcctgatggc gatggcgttc atgtgcttct cggggatcag cctgtaggcg gaaaacatgg
     2641 aacacccggt cctctacatc ctggcgagcg tggcggtgtt cacggggatc atcctgttcc
     2701 tcaccgccgg cctgatgatc gcgtcgcgcc gcctggcgcc gagcgggccc gtgaccatcg
     2761 acatcaacga gggctacaag aagctgacgg tggacgcggg ggccacgctg ctgtcctcgc
     2821 tggcttccga gcgcatcttc ctgccgtcgg cctgcggcgg cggcgggacc tgcgcgatgt
     2881 gcaagtgcac cgtgaccgac ggcggcggga gcatcctgcc gacggagacc gggcatatca
     2941 gcaagcagca ggccaaggag gggctgcgcc tggcgtgcca gttgaaggtc aagcgcgacc
     3001 tcaagatacg gatcccgtcc gacgtgctcg agatccggaa gttcgagggc acggtgcggt
     3061 cgaaccgcaa cgtggcgacg ttcatcaagg agctggtgat cgaccttccc gcgggcacgg
     3121 acctcaagta ccgggcgggc gggtacatcc agatcgacat cccgccgtac gagctggcct
     3181 accgcgattt cgacatcgac gagcggttcc gcgacgcgtg ggacgcgttc aggctctggg
     3241 acctgaaggt gaaaagcgcc gagcccatct tccgagccta ctccatggcg agctatcccg
     3301 ccgagggcaa cctggtgaag ctcaacgtcc gcatcgccac gccgccgccc gggatgaacg
     3361 cgccgccggg gatcgcgtcg agttacatct tcggcctcaa gcctagcgac aaggtcgtgg
     3421 tcagcgggcc gtacggcgag ttcttcgcca gggagagcga ccgcgagatg ctgtacatcg
     3481 gcggcggcgc gggcatggcc ccgctgcgca gccacatcat ggacctgctg gtcacgaaga
     3541 aatcgaatcg caagatcagc ttctggtacg gcgcgcggtc gctgcgcgag atgttctacg
     3601 aggaggactt ccggaagctg gagcgggagc acccgaactt caaattccat atcgcgttga
     3661 gcgagccgcg acccgaggat gactggaagg ggcccgtggg tttcatccat tcggtcgtcc
     3721 acgaccgcta cctggcgaac cacgaagacc cgtccgagat cgagtattac atgtgcggcc
     3781 cgccgcccat gatcgccgcc gtcaacgcca tgctgtacaa tgtcggcgtg gacaaggaca
     3841 tgattgccta cgacgagttc tgacgcatgc tcgtccagcc ccgcagaatg aatccatgga
     3901 agctgctctg ggtcctggcg gtgatcttgg ccttcgccgc cttcacctgg cgccgcgatc
     3961 cgccgggtcc gaaaaatccc tcgtgggccg gagagacgat gggcaccctg tactcgatca
     4021 agctggccga ttcgccgttg gggcagggcg cgctgcgcga attgcaggcc gaggtggacc
     4081 gctgcctggt cgaggttaac cgcgaaatgt cccactacca accggacagc gaactttcgc
     4141 agttcaatcg tcacgaatcc gccgagccgt tcccggtgtc gcccgggttc gcgagcgtag
     4201 tccgctttgc cctcgagtta aaccggcggt cgggcggtgt tttcgatccc acgctgggcc
     4261 cgctcatcga cttgtgggga ttcggcgcgc cgggtcatgt gagcgagccg ccttcgccgg
     4321 cgcaggtcga ggaggcgcgg gcgcggatcg gcggacagca tctctccgtc actccggcgg
     4381 gacagctggt caaggggatt cccagccttc gcctgaacct gggcgccgtg gccaagggct
     4441 acggggtgga cgaagtggcg cgggtgatcc gcgcgaacgg catcagcaac ctgttcgtcg
     4501 agatcggcgg cgaagtcgtc accgccggcc acaatgccga ggggcaagcg tggcggatcg
     4561 gcgtggatgc gccgcgcccc gatgcgctgc ccgggcagga gttggagacc atccttcacc
     4621 tgtccggcct cgcggtcgcc acgtccggcg actaccggaa ctacttcgtg gacggcgcag
     4681 ggatgcggtt cacgcacatt ctcgatccgc gcaccggccg gccgatccag cataacctgg
     4741 ccagcgtctc cgtggtggcg ccggattgca tgacggcgga tggcctggcc acgacgctgt
     4801 tcgtcatggg cgccgaggag gggatgaagt ggatcgggtc ctggccgggc gtcgaggcgc
     4861 ttttcatcct gcgggagccc gatggccggt tcgtctgccg gccgaccccg ggcttcgtgg
     4921 ccttgaccgg ctgtgcgctc cccgagtgat ccccgcgcgt ccgggcgccg ggtcgtcttc
     4981 ctggctccgg ctgttctgct catgccggct cgttcagggc ccccgcccgc caatcctgcc
     5041 ggacttgtat tcatatatag ccatatggct atatatgaat aaaagaccgg ctttttcgca
     5101 acgcggagcc gggcgggcca gcggggacgg cgacgggcaa tctggctcgg cgcatcgttg
     5161 ccgccaatag ccgcgcttgc gccggccgcg ggcaggcctt atcctttgag cccatggaca
     5221 aaacgcactc gggctctcat gtcggattct gggccgtcat cctgctgctg ctgatcgcgc
     5281 tcgtcggcag cctggcgatg aacgccgggc ttctcctggg ccgcgccctg acgggctgga
     5341 gcccggaaga ggaggaagcc gaagacgagt ttcccgcctt cgacgaggtc tggtcgtggg
     5401 gggagggcga ggccaaggtg gtccgcatcc ccatccaggg cgagttgttc cactatgtct
     5461 cggagggctc ccttttctcg ccctcgttcg acccgatcga atcaatcctc cggcagatcc
     5521 gcgcggcgca gaacgacgag gacgtcgtgg ccctcatcct ggaagtcagc tcgccgggcg
     5581 gcgacatgac gtccgccgac gaaatctacc aggcggtcca gaaattcaag gccagcggcg
     5641 aggatcgccg cgtgatcgcc cacgtccagg acctggcggc ctccggcggg tattacgcca
     5701 tcctgccggc cgaccggatc gtcgccgagc ccaccgccat gctgggctcc atcggcgtga
     5761 tcatgcagac gctcaactgg aagacgctcg gcgagaagct gggcatcgcc gataccacca
     5821 tcaagtccgg cgagcacaag gacctgctca atcccttccg cgaagtgtcg gaggagcagc
     5881 gggcgatcct ccagaagatc gtggattccg cgtacagccg gttcttcgat ctcgtggtga
     5941 ccaaccgcgg cgtcgagccg gcgaagctgc gcgagctcgc ggacgggcgg gtcttcgacg
     6001 cgtccgtagc cctggaaggg gggctgatcg acgacatcgg ctactggacc gacgtgctcg
     6061 accagttgcc ggaagtgacc ggggaggact ccgtgaagat cgtgcggtat tcccactcgc
     6121 tgactttctc cgactggctg ggctccgtgc atgcgcgttt ccggtggccc cgctggatcc
     6181 agtcggacgc cccccgcctg atgtacctgt ggcggccgtg agcgccggtc cggcctctcc
     6241 ggggatggga aatgcgcgcc cgttttccaa gcaacggaaa aactaggcat ccggcggggt
     6301 ttcgctagaa taaccaagga gagatgctct accgcctcat agtgttgagc gggccgctca
     6361 agggccgccg cctgaccgtg gagcgcgccc cgaccacggt cggccgcgat cctgcctgtt
     6421 ccattcatct ggacgacgag gaggtggcgc tccggcacgc cgtgttcgag cataacgaca
     6481 ccgggctggt cgtccgcgat ctcggcacga tgaaccgcgt gctggtgaac aagcgggagg
     6541 tccgcgaggc gcacctgcgg catggcgacg agatcgagat cggccggacc cgctttcttg
     6601 tccaggccct cctccagacc gaggtcgagg cgccggcgga agaggagggc gagccccgcc
     6661 gcgggctggg cgcgacgccg agggtcctgc tgctgcttct cctctttctc gcgctgttca
     6721 tcgcgtacca gcgatgggcc gcccagcgca cccgcccgac gaccgcgttc cgtcccccgg
     6781 tcgagacgcc ggccgtcgcg ccgtccaatg tccccgcccc gatcgagccg cccgccttgc
     6841 cgccccagat ggaggaagaa ctccggtcca tgcgggcgac gctggccacg atcaaggaga
     6901 ccgtcgagga cctgggcacc cggaccgcca tgccgcccgc cgcgccggtc agcccggcgg
     6961 tcccgtcgga gcccccggtc ccttcgccgg actacacggc gtggctcgcc atcgagagcg
     7021 tcgaacaggt caagctgccc caggacgact cccccgacga agcccgcctg ctgcgcctgc
     7081 gcctgcgcgc ggcgccgggc gtgaccatcg agcccgacgg cgtgcggatg gaggtccagc
     7141 tgttcatccg cgaggaggag acgctgcgca tcttccccgc ccgcgtcgag gtgctcgtca
     7201 gcccgcccga atttcacgac ggggttctga ccaacggcgg ctcggcgatc gccacgatgc
     7261 agtgccgggt ccccggcgcc gcgggccgcc tgggccggta ctacgggttc cggctgaaac
     7321 tgttctacaa ggacgtgctc caggccgagg tggaccggcc gcgcaccctc gccgccgacc
     7381 tgtggcgcga gaccgacggc gagctttctc cggactgatc cgccatgcgc gagaagatat
     7441 ccgcctgcgt catctgcttc aacgaggagc ggaagatacg ccgctgcctc gccagcctct
     7501 cgtggtgcga cgagatcatc gtgatggaca gcttcagcac cgaccagacc ccggccatct
     7561 gccgcgagta cacggaacgg ttctaccagc acgagtggct cgggtacatc gcccagcgca
     7621 acatggtccg cgacctggcg ttgtacccgt ggatcctcta cctggattcc gacgaggagg
     7681 tctcgcccgc gttgcgcgac gagatcctgg ccgagttcga gcgtggcacc gatcccttcg
     7741 tgggctacga attcccgcgc caggtgtatt acatcgggcg ctggatccgg cacggcgagt
     7801 ggtaccccga cgtcaagctg cgcctgttcc gcaagagccg cggccgcacg gaggggatcg
     7861 agccgcacga caccgtggtc gtcaagggcc ccgtccagcg gctccaggcg cccgtctggc
     7921 actataccta cgacgatttg cgcgaccaca tggacaccgc caaccggttt tccacgatct
     7981 cggcccagca gaagttcgtg caggaagtgc cgttccggtg gctggacctg ttcctgcggc
     8041 cgcccttcca tttcctcaag ggctacgtcc tgcgggccgg gttcctcgac gggatgcacg
     8101 gcttcatcat tgccgtcatc agcgcctacg ccgcgttcgc caagtatgcc aagctgtggg
     8161 agctcgaacg ccggcacaag caccacttcc gcgaactgcc gtagggcagg gtcagccttt
     8221 ttccagccgg tactccagca tcttgtcccc gtcgaaaagg aaccggccat ggaatggcgt
     8281 gccggccagc atcaggggaa tccacacgcg gtcatcctcc cacatgcggt cgaaggggat
     8341 ttggtccagc ggggtccaca acgggatcgc ctcgccggtc tcctgcggct cgccctcgat
     8401 gccggtcgcc gtgaaaacgt agccgtggat ggaaaacccg tccacgaact ggaacatcag
     8461 ttcgcccgcc tcgcggacgc cggtaggggt gacgaccagc tcctcctgca cctcgcggat
     8521 cgccgcctgc cggggcgtct cgcccgggtc cagccggccg ccgggtccgt tgatcttgcc
     8581 cgcgcccagc ccctttttct tgtggatcag caacacctgc ccgtcgcgga tcacgaacat
     8641 cagcgtcgcg cgctcggtcg ggttccagtg cgcccagtcc acctcggaga aatgcgaata
     8701 tcgtttcatg gaatgaccag gaaatacacg aaagacacgg aaacggaaag gctcttattg
     8761 acccgccgcg gcccgccgga tcagttccag gaacgagcgt accttgtcgt tgccggggtc
     8821 cgcgcggaga caagctgcaa tcacggcctc cgcgtcgttg aatttccgcc gttgcatgag
     8881 cacgtcggcc aagcggtagt tggcctccgg gctgtcgggg tacagggcta gggcctggcg
     8941 aaaagcatat tcggcctcat cgaacaggcc ccgataggca tagaggccgg cgatggcgca
     9001 acgaagcttg cagaagctcc tgcgagccgc cacgtcccgc aggaaggcag gccggtcgag
     9061 caggcgccgg gaataccagt tccagaatgc gcggtcctgg tttacaagct ccggcgggag
     9121 cgacggcagc ggctcggcat ggagcctcat gatcaggccg tggggcgcga ggtgcgggaa
     9181 catccacggg atgacgtagc tctcctcgat gtagaaggcg tgccggtcct tgttcgcctc
     9241 gaaaaaccgc cgagccagta tcccattaat cgccatgacg ccggctacgc cttggacggt
     9301 gacccgatcg ccctggggct tggcgccggt gcccggcggc agacggcccg cctgaagttc
     9361 ttccatgtac tgatagaatg cggcgtccgc atccgccggg gacgggatac gtatagcgcc
     9421 tccgtaaagg tcccgcaggg cattcaggta ggtacggtcc gcgagcgcat tctgggtcag
     9481 caggaatacg tcgggccgga tcccggcgga gaagatcatg tacgtgggca cgaaccgtcc
     9541 ggggtccgtg ccgccaaaca ggagagcgcc gcgttccagc ggcggcggat agtcgcgggc
     9601 gggcagcggt gtttctccgg gttggaggtc cgcgacgatg ccgccgaggc cttccagttg
     9661 atggcgcccg aactgccagc cgaagtcatg accactctga ctgctgccgc catacacgcg
     9721 gaccagctcc cggtcccgct gctgacggac cagtggcact aatgggagca ggagagcggc
     9781 gaagaacacc gaggccagga cgggccggtg ccgggaggcc gcgctgaaga tccatccgag
     9841 tc
//
LOCUS       DAOOTK010000114         9764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4508_length_9764_cov_5.899990, whole genome shotgun sequence.
ACCESSION   DAOOTK010000114 DAOOTK010000000
VERSION     DAOOTK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4508_length_9764_cov_5.899990"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK634_11405"
     CDS             <1..601
                     /locus_tag="PK634_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98383.1"
                     /translation="WRRDIPPLQQESARPTRLDVHVPPRRLAVVSEESLHVLNPADGE
                     PLRVIPLPAEAMEPDAMDRRPLLVYLQDGSNAVVAALKTKYEVTERSETSRSYRTVET
                     SVVGFSDDGGVLWTNTLEQGMGALQVLALPVAADRDYLVVATWNQLLLLNPKGEVKLD
                     QPVGFREHWSIRPGADGKYEVVVRGEDVAAYEWNLRAGD"
     gene            633..1511
                     /locus_tag="PK634_11410"
     CDS             633..1511
                     /locus_tag="PK634_11410"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOW98384.1"
                     /translation="MPDTTIAELLRRAARPLLSFEFFPPKDEKGLDTLRKAAGQLVAA
                     RPDFVTVTYGAGGSTRERTLEVCELLRGMGFAVVMPHLTCVGSSRAELLEYADELHRR
                     GYRNIMTLRGDPPKGETVFRVAKDGLAHASDLVALLKGRHADFCCGVAGYPEKHPEAA
                     SLPADIANMKAKLAAGGDFITTQLFFENRFYADYVRRCREAGVTQPILPGIMPALSHR
                     QIQRFTAVCGATFPGPLATRLAAAADDAAAAQVGMAWAQEQVEQLLRDGVPGIHLYVL
                     NRAHAAPLIASLRRAG"
     regulatory      1574..1757
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1787..3361
                     /locus_tag="PK634_11415"
     CDS             1787..3361
                     /locus_tag="PK634_11415"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOW98385.1"
                     /translation="MRKSLVRFLTTGLATGLPVVAPALNADFEDHPLPAQSAWNGSDL
                     SGGVTSRTVFFRNRYDPAWGNWSGFALSNVNDTNTAGWLNQYAVFAGTGLGGAGTYAV
                     AYDDTYGEEADIITLPLPAWVRGFYVNNTTYAALTMRDGDEYGFSKKFGGATGDDPDW
                     FRLTVTGRDLDGQVVGVTNHYLADYRFTNNAQDFIQSAWQWVDLSSFGAEVATLHFSL
                     SSSDTGAWGMNTPAYFALDGLVFTYAPAAGECSSAAVAWTERRIVTWATGWTQYNAGP
                     GCDPSWQTPGNAMGPSDVEETNGIVCLGDGGSITMTFDVPLADGPGPDFAVFENALND
                     FFLELAWVEVSSDGNHFSRFPNQTLATNTVPFWGGEVQSTNIAGLAGQYRRQYGTPFD
                     LRQLAGVSPLLDVMDVRHVRLVDIVGDGSCSDSFGRAIYDPYPTEGSAGFDLDAVGVI
                     NYRMDCEPASADRGRFACQAFTNWLYELQASGDLAGDGWTTVLSVTGRNERVELDDPA
                     PPASGRFYRLRRTLLP"
     gene            3358..4218
                     /locus_tag="PK634_11420"
     CDS             3358..4218
                     /locus_tag="PK634_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOW98386.1"
                     /translation="MTACAPWGARAPAGRAEFSNDWKNRKPSFPNIGKPGFSLVELLV
                     VVAILFVLAAALVPWVIRSIEAGRRAQCGSNLRQLFVANAAYAAENGSYVAAAPDIMG
                     PNRRRWHGVRSSGSGAFEGSLGPLARFLEDGRARRCPSFPDYRRDAAANAFESACGGY
                     GYNDRGVGSRVYLLGYGSAAMQQGMFPGAIRNPARTVMFADAAFGQPYNDPRWFIEYS
                     FAEAYYFVGRTPAQLYGPSRPSVHFRHGGRAGVVWCDGHVTWETQALGNPASGLGWFG
                     PADNSLFDPL"
     gene            4369..4944
                     /locus_tag="PK634_11425"
     CDS             4369..4944
                     /locus_tag="PK634_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOW98387.1"
                     /translation="MNSIELSPTLWRTCRVLAGPTRLRMFGRLCRDPHQSVGEVAAAL
                     KIGRSRASQELRRLQARGLLKATRHGRWVRYAPAADPRVPSAKPIVQALRTALARSRP
                     GADAECLRIASALTHPRRLRIAQALLVQPLDFSRLVTETRMPRISLSRHLRHLRARGL
                     VVRKQGVYHFIADPHPLARCLLGLLAETPQR"
     gene            4970..6076
                     /locus_tag="PK634_11430"
     CDS             4970..6076
                     /locus_tag="PK634_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOW98388.1"
                     /translation="MKVCVDIQAAVAQRAGVGRYTKSLVEHLGLQAGDDALSLFFFDF
                     KGRGDPFPVPNATRQAVRWCPGMLVQQAWKRLDWPPFDWFAGRADVYHFPNFIVPPLA
                     RGKAVVTIHDLAFQRFPETTERRNLDYLTAQIGKTVQRADAIIAVSNFVAHEIHERLG
                     VPESRIFAIPSGMSSAWAAPCADSVAATQRELGLDRPYLLTVGTLEPRKNIGFLVEVF
                     ERMKFDGDLVIAGAMGWKYEPILDRIRRSTRAPRIRHLRYVTEKHLAALYAGAELFVF
                     PSLYEGFGFTPLEAMACGVPVVAATTGSLPEVLGEAAELVAGYDAGEWTARADRLLTD
                     TARRAELKERGFRQARRFSWDETARRTWQVYRGL"
     gene            6123..7268
                     /locus_tag="PK634_11435"
     CDS             6123..7268
                     /locus_tag="PK634_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOW98389.1"
                     /translation="MRIGLDARWIFPELSGIGTYTTELIREFARLETGHEFVLFFRDP
                     AIRDRVWRVAGLDGHPRFRAQMIPWGVFSPWSQLRLPAVLRRLALDVFHSPNYMIPFR
                     AFPRGRAHAVQCVATVHDLIPLLFPEHTPRALKTRLFPLFRRVMFEVGARADAILTVS
                     EASRADILRSLRIPGEREARVRVVYNGVAPEYRPALRRPAPHKTILYVGRFDPYKNVE
                     GLLEAFARLRSRGGPAARLKIVGAPDPRYPEAPRRARELGLEGLVEWAGYTGGADLVD
                     AYQQADVFVLLSRYEGFGLTLLEAMACGAPAVCSNIGALREVAGEAALFVDGADPEAA
                     ADALGRVLEDRGVAAELSRKGLARAREFTWARAAAEILRVYEEVARS"
     gene            7265..8401
                     /locus_tag="PK634_11440"
     CDS             7265..8401
                     /locus_tag="PK634_11440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98390.1"
                     /translation="MNAIPPVAFPSGWRDLRVVLCHDWLNGMRGGERVLEWLCRGFPG
                     APVYTLLCKPDAISADIRAHPLRASWLQRVPGIQRWYRYFLPLFPGAIERLRAPDADL
                     LISTSHCVAKGLVPPPGARHLCYCFTPMRYAWVFYEEYFGSNPLKKRILAPRLAKLRE
                     WDRRASDRVDRFVTLSRHVQDRIRRFYGRESAVVYPPVDTGFFTPAAVEPGGFDLVVS
                     ALVPYKCLDLAVRAYTWLEYPLKIVGTGTEYDRLRRLAGPSVQFLGWRSDEEIRDLYR
                     RCRFLVFPGEEDFGIVPVEAQACGRPVVAFARGGVLETVRENVTGVFFKEQTDQALLA
                     AIEVAAARRWDPAAIRAHAEQFGVQAFVDGMAREIGLCMDRRAG"
     gene            8405..>9764
                     /locus_tag="PK634_11445"
     CDS             8405..>9764
                     /locus_tag="PK634_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98391.1"
                     /translation="MAAPDRPVSRRGLVLWGMLAYLWGAVVHLRLGAYRPGLIWGDSG
                     DGLFAVWVLRHGADWLRGAAPLADGRIFWPSHALSAFWSELYLLMAPVFMLLEKLTAS
                     SFAAAYALGVLLSVVAFFACLFFVEQVLATARDPDRPPVPGWVPPLVAYLAWFSIGLL
                     QIFKIQVLPSFWLLVMTGGMLAWRRTGLARWMLLVWASLAAVFLSSSYFAAAGVLLFA
                     AWAWVEWFRDPRVWWRVFLKLWPALLAGAAAMGAVALIMSRPGVQRYSPEYIHSMAVG
                     WLDLVRPRDGLVSAGWLRALTGAAENPSNIGAFLGWGWIVAAAAWLARARREVWSFLK
                     QALRSRWFVALALVLLAGRLKIREARALLAIPGFLAVALALLVSAAAVGRHGRDKPRL
                     YAMGLLALFAVLLFGLSFGPAGWYVGHPLHPSVWSALRLTIPGVAAMRAIDRMAVVAV
                     VFA"
BASE COUNT         1352 a         3222 c         3374 g         1816 t
ORIGIN      
        1 gtggcggcgc gacatcccgc ccctgcagca ggaatcagcc cgtccgacgc gcctggatgt
       61 ccatgtgccg ccccgccggc tggccgtcgt ctcggaggag tcgttgcacg tgctgaatcc
      121 ggcggacggc gaaccgctcc gggttattcc tctgcccgcc gaggcgatgg agcccgacgc
      181 gatggaccgt cgtcctttgc tcgtgtacct gcaggacgga tccaacgccg tggtggcggc
      241 tctgaagacg aagtacgaag tgaccgagcg gagcgaaacc agccggtcct atcgcaccgt
      301 ggaaaccagc gtggtgggtt tttccgatga cggcggcgtc ctgtggacga acacgctgga
      361 gcagggcatg ggcgccctgc aggtcctggc cctgccggtc gcggccgatc gcgactatct
      421 cgtcgtggcc acgtggaacc agctcctgct gctgaacccg aagggcgaag tgaagctcga
      481 ccagcccgtc ggtttccggg agcattggtc gatcaggccg ggggcggacg ggaagtacga
      541 ggtcgtcgtg cgcggcgaag acgtggcggc gtacgagtgg aacctccggg cgggcgattg
      601 acgtccggcg gaaaaccgct agggtagcct ccatgcccga tacaaccatt gccgaattgc
      661 tccggcgcgc cgcgcgcccg ttgctctcct tcgagttctt tccgccgaag gacgaaaagg
      721 gcctggacac gctgcggaag gccgccggac agctcgtcgc cgcgcgcccg gatttcgtga
      781 cggtgaccta cggtgcgggc ggttcgaccc gcgagcggac gctggaggtg tgcgaactgc
      841 tgcgcgggat gggcttcgcc gtcgtcatgc cgcacctcac ctgcgtcggc tccagccgcg
      901 cggaactctt ggaatacgcc gatgagttgc accgtcgagg ctatcgcaac atcatgaccc
      961 tgcgcggcga tccgccgaag ggcgagaccg ttttccgcgt ggcgaaggac ggcctggcgc
     1021 acgcctccga cctcgtggcg ctgctcaagg gccggcatgc cgatttctgc tgcggggtcg
     1081 cgggctaccc ggaaaagcat ccggaggctg ccagtctgcc ggcggatatc gcgaacatga
     1141 aggccaagct ggccgccggc ggggatttca tcaccacgca gttgttcttc gagaaccgtt
     1201 tttacgccga ctacgtccgg cggtgccggg aagcgggcgt tacgcagccg atcctcccgg
     1261 ggatcatgcc ggcgctgtcg caccggcaga tccagcggtt cacggcggtc tgcggcgcga
     1321 cttttcccgg cccgctggcc acgcggctcg ccgcggccgc ggatgatgcg gccgccgcgc
     1381 aggtcggcat ggcctgggcg caggagcagg tcgagcagtt gctccgagac ggcgtgccgg
     1441 ggatacatct ctacgtcctc aaccgggcgc acgccgcgcc gctgatagcc tcccttcgcc
     1501 gcgccgggta ggggccttcg cttgccgagg actgcccggg cttgacgtga ggggcgtggc
     1561 tccggtaccg tcctcccggt tctttgttga aactgtgcgc gggaaaagcg aacggaagtc
     1621 cgtgcgaatc ggacgcggtc gcgccgctgt aaccggctac aaaccccaca agccacggga
     1681 ggcaacaact ccttccggaa ggcggggcga ggcctgaagc cgggagccag aagacgagtt
     1741 tttcccgttg cccatatgcc cccgcacgag gggcagaaag gcgtccgtga gaaagtccct
     1801 tgttcgtttc ctgaccaccg gtctggcgac cggtctgcct gtggtcgccc ccgccctgaa
     1861 cgcggatttc gaggatcatc cgctcccggc gcaatccgca tggaacggct ccgatctctc
     1921 cggcggcgtc accagccgga ccgttttttt caggaaccgc tacgatcccg cgtggggcaa
     1981 ctggagcggg ttcgcgctct ccaacgtcaa cgacaccaac acggccgggt ggctcaacca
     2041 gtacgccgtc tttgccggga cgggtctcgg gggagcgggc acgtatgccg tggcctacga
     2101 cgacacgtac ggcgaggagg cggacatcat cacgctcccg ttgcccgcct gggtgcgcgg
     2161 cttctacgtc aacaacacca cgtacgccgc cctgaccatg cgcgatggcg acgagtacgg
     2221 tttttccaag aagttcggcg gagcgacggg cgacgacccg gactggttcc ggctcaccgt
     2281 caccggccgc gacctcgacg ggcaggtggt cggcgtcacg aatcactacc tggccgacta
     2341 ccggttcacg aacaacgcgc aggactttat ccagtccgcg tggcaatggg tcgacctctc
     2401 gtcgttcggg gcggaggtcg ccacgctgca tttttccctg tcgtccagcg acacgggcgc
     2461 gtgggggatg aacacgccag cctatttcgc gctcgacggc ctggtcttca cctacgcgcc
     2521 ggcggcgggg gagtgctctt cggccgccgt ggcgtggacg gagcggcgca ttgtgacatg
     2581 ggcgaccggt tggacccagt acaatgcggg acccggctgt gatccgtcat ggcagacgcc
     2641 ggggaatgcc atggggccgt cggacgtgga agagacgaac ggcatcgtct gccttggaga
     2701 cgggggttcc atcaccatga cgttcgatgt cccgctggcc gacggtccgg gcccggattt
     2761 tgccgtgttc gagaacgcgt tgaacgattt cttcctcgag ttggcctggg tcgaggtgtc
     2821 aagcgacggg aaccactttt cccgttttcc gaatcagacg ctggcgacca acaccgtgcc
     2881 gttctggggc ggggaggttc agtccacgaa tatcgccggg ctggccggcc agtaccggcg
     2941 ccaatacggc acgccgttcg atttgcgaca gttggccggc gtatccccgc tcctggatgt
     3001 catggatgtc cgccacgttc ggctcgtgga catcgtcggc gacggctctt gcagcgactc
     3061 cttcgggcgg gccatttatg acccgtatcc caccgagggt tctgcgggat tcgacttgga
     3121 tgccgtgggc gtcatcaact accgcatgga ttgcgagccg gcttcggccg acaggggccg
     3181 ttttgcctgc caggccttca ccaactggct ctatgaattg caggcttccg gggatctggc
     3241 gggagacggg tggacgaccg ttctatcggt cactggccgc aatgagcggg tcgagctgga
     3301 cgatcccgct cctccggcat ccggccggtt ctatcgtttg cggaggacgc tgctgccgtg
     3361 acggcgtgcg ccccatgggg ggcccgcgct cccgcgggcc gagcggagtt ttccaatgat
     3421 tggaaaaacc gcaaaccttc ttttccaaac attggaaaac ccgggttttc cctggtcgag
     3481 ctgctcgtgg tggtggcgat cctcttcgtg ctggcggcgg ccctggtccc atgggtgatt
     3541 cgaagtatcg aggccggccg ccgggcgcag tgcggatcga acctgcggca gttattcgtc
     3601 gccaacgcgg cctacgcggc ggagaacgga agttacgtgg cggcggcgcc ggacatcatg
     3661 gggccgaacc gccggcgctg gcacggggtg cggtcgtccg gttcgggcgc gtttgaaggc
     3721 tcgttgggcc cgctggcgcg gttcctggag gatgggcggg cccggcgctg cccctccttt
     3781 ccggactacc ggcgggacgc ggcggccaac gccttcgagt cggcgtgcgg cgggtatgga
     3841 tacaacgatc gcggggtcgg ttcgcgggta tatcttctgg gatacggctc ggcggcgatg
     3901 cagcagggga tgtttcccgg ggcaattcgg aatccggcgc ggacggtgat gttcgccgac
     3961 gcggccttcg gtcagccgta caatgacccg cgatggttca tcgagtactc cttcgccgag
     4021 gcgtattatt ttgtcggccg cacgcccgcg caactgtatg gcccctcgcg gccgtccgtt
     4081 cacttccgtc acggcgggcg ggccggcgtg gtctggtgcg acggacacgt cacgtgggag
     4141 acgcaagcgc tgggcaatcc tgcctccggc ctcggctggt tcggaccggc cgacaactcg
     4201 ttgtttgatc cgctgtagcg ggcggtcctc gcggcgagga gccccgattc gttgaacagg
     4261 ccaggtatca cagtttataa actgtgatat tatggccgcg ggatagcgcg atttcgttag
     4321 gaaatattga tgattgacgc gtgtatcaca gtttataaac tgtgatacat gaactccatc
     4381 gaattgagtc cgacgctttg gcgcacatgc cgcgtcctgg ccggccccac gcgtctgcgc
     4441 atgttcggcc ggttgtgccg cgatccccat caatcggttg gcgaagtggc ggcggccctg
     4501 aagatcggcc gcagccgcgc cagccaggaa ctgcgacggc ttcaggcccg gggcctcctg
     4561 aaggcaaccc gccatgggcg ttgggtccgc tatgccccgg cggccgatcc ccgcgtgccg
     4621 tccgccaaac cgatcgtgca ggcgttgcgg accgccctgg cccgctcccg cccgggcgcg
     4681 gatgccgagt gcctgcgcat cgcgtccgcg ctgacccatc cgaggcgctt gcggatcgca
     4741 caggcccttc tcgtccaacc cctcgacttc tctcgcttgg tgaccgagac ccggatgccg
     4801 cgcatttcgc tgtcgcgcca tctgcgccac ctccgcgcgc ggggactcgt tgtccgaaag
     4861 cagggcgtgt atcatttcat tgccgatccg catcccctgg cgcgatgcct gctgggattg
     4921 ctggcggaga cgcctcagcg gtagctgtgc cgcgggacgg ccaaacccca tgaaagtatg
     4981 cgtggatatc caggcggccg tggcccagcg ggccggagtg ggtcgctata cgaagtccct
     5041 ggtggagcac cttggcctgc aggccgggga cgacgcgctg tcacttttct tttttgattt
     5101 caaggggcgg ggggacccgt ttcccgtgcc gaacgcgacc cggcaggccg ttcgctggtg
     5161 ccccgggatg ctggttcagc aggcgtggaa gcgactcgac tggcccccct tcgactggtt
     5221 cgccgggcgg gccgacgtgt atcactttcc caatttcatc gtcccgccgc tggcgcgagg
     5281 gaaggccgtg gtgacgattc acgacctcgc cttccagcgg ttcccggaga ccaccgagcg
     5341 acgcaacctg gactacctga cggcgcaaat agggaagacc gtccagcggg ccgacgccat
     5401 catcgcggtc tccaacttcg tggcccacga aatccatgag agactcggag ttcccgagtc
     5461 gcggattttt gccattccat ccggaatgtc ctccgcctgg gcggcgccct gcgccgattc
     5521 cgtggccgcg acgcagcgcg aactcggact cgaccgcccg tacctgctga ccgttgggac
     5581 gctcgaaccc cggaagaata tcggatttct ggtcgaagtg tttgaacgga tgaagttcga
     5641 cggcgacctg gtgatcgccg gggcaatggg ctggaaatac gaaccgatcc tcgatcgcat
     5701 ccggcgatcg acccgcgcgc cgcgcatccg gcacctgcgc tacgtgacgg aaaagcacct
     5761 agccgccctc tatgccggcg cggagctgtt tgtcttcccc tcgctgtacg aaggcttcgg
     5821 ctttactccg ctggaggcca tggcctgcgg tgttcccgtc gtggcggcca ccacgggctc
     5881 gctgcccgag gtcctcggcg aggcggccga actggtcgcc ggatacgacg ccggggagtg
     5941 gaccgcgcgc gcggatcgac tgctgaccga cacggcccgc cgcgcggaac tcaaggagcg
     6001 cgggttccgg caggcgcgcc ggttcagttg ggacgagacc gcccggcgca cctggcaggt
     6061 gtaccgcgga ctgtagcctt ttctttaccg ggctttttct gtatgtcgag ggccggtgat
     6121 ttatgcgaat tggactggac gcgcggtgga ttttcccgga gttgagcggg atcgggacct
     6181 acacgacgga actgatccgc gagttcgccc gcctggagac cgggcacgag ttcgtgctct
     6241 tcttccgcga tccggcgatc cgcgaccggg tgtggcgagt cgccggcttg gacgggcatc
     6301 cgcgctttcg agcgcaaatg atcccgtggg gcgtgttctc cccgtggagc cagttgcggc
     6361 tgcccgccgt cctccgacgg cttgcgctcg atgtcttcca ttcccccaat tacatgatcc
     6421 cgttccgggc tttcccccgc ggccgcgcgc acgcggtgca gtgcgtcgcc accgtccacg
     6481 acctgattcc gcttctgttc ccggaacaca cgcccagggc tctcaaaacc cgcctgtttc
     6541 cgctgttccg gagggtcatg ttcgaggtcg gcgcgcgggc ggacgccatc ctgacggtca
     6601 gcgaagcgtc ccgggcggac atcctgcgca gcctgcgcat cccgggcgag cgggaggcgc
     6661 gggtgcgtgt cgtgtacaat ggcgtggccc cggaataccg ccctgcgctc cgccggcccg
     6721 ctccgcacaa aaccatcctc tatgtcggcc ggttcgatcc ttataaaaat gtcgaggggc
     6781 tgctggaggc ctttgcccgg ctccgctcgc gcggggggcc cgctgcgcgg ctgaagatcg
     6841 tcggcgcgcc ggatccgcgc tatccggaag cgccgcgccg ggcgcgggag ttggggctgg
     6901 agggcctggt cgagtgggcc ggctacacag gcggggcgga cctcgtggac gcataccagc
     6961 aggcggacgt cttcgttctg ctgtcgcgct acgagggctt cggcctgacc ttgctggagg
     7021 cgatggcctg tggcgcgccg gcggtgtgca gcaacatcgg cgcccttcgc gaggttgccg
     7081 gtgaagcggc gctcttcgtg gatggcgctg atcccgaggc cgcggcggac gcgctggggc
     7141 gggtgctcga ggatcggggc gttgcggcgg agttgagccg caagggcttg gcgcgcgcgc
     7201 gggagttcac ctgggcgcgc gcggccgccg agattctccg ggtctacgag gaggtcgccc
     7261 ggtcgtgaac gcgatcccgc ccgtggcctt cccgtccggc tggcgcgacc tgcgcgtcgt
     7321 gctctgccac gactggctca acggcatgcg cgggggcgag cgggtgctgg aatggttgtg
     7381 ccggggcttc ccgggcgcgc ccgtttacac gttgctctgc aagccggacg cgatttccgc
     7441 ggacatccga gcgcaccccc ttcgcgcgag ctggctgcag cgcgtgcccg gcatccaacg
     7501 atggtaccgg tatttcctgc cgctttttcc gggcgccatc gaacggctgc gcgcgcctga
     7561 cgccgacttg ctcatcagca cgagccattg cgtggccaag ggcctggtcc ctccgcccgg
     7621 ggcgaggcac ctgtgctatt gtttcacgcc catgcggtat gcgtgggtct tttacgagga
     7681 gtatttcggc agcaatccgc tgaagaaaag gatcctggcg ccgcgcctgg cgaaactgcg
     7741 cgagtgggac cgccgggcgt cggatcgcgt ggatcgtttc gtcaccttaa gccggcacgt
     7801 ccaggaccgc atccgccgct tctacggccg cgaatcggcc gtcgtctacc cgcccgtgga
     7861 caccgggttt ttcacgccgg cggccgtcga gcccggcggc ttcgacctgg tcgtttccgc
     7921 cctggtcccg tacaagtgcc tcgacctggc cgtgcgcgcg tacacgtggc tggaataccc
     7981 gctgaagatt gtcggtacgg gcacggagta cgaccgcttg cgccggctgg ccgggccgtc
     8041 cgtccagttc ctgggctggc ggtcggacga agaaatccgc gacctgtacc ggcgctgccg
     8101 cttcctggtg ttcccgggcg aggaggattt cggcatcgtc ccggtagagg cccaggcctg
     8161 cggccggccg gtcgtcgcct tcgcgcgcgg gggcgtgctg gaaaccgtcc gcgaaaatgt
     8221 cacgggcgtc tttttcaagg agcagaccga ccaggcgctg ctggccgcca tcgaggttgc
     8281 cgccgcccgc cgctgggatc cggccgcgat ccgcgctcac gcggagcagt tcggggtcca
     8341 ggcgttcgtc gacggcatgg cccgtgaaat aggcctctgc atggaccgcc gggcggggtg
     8401 atccatggcc gctcccgata ggccggtttc gcggcgaggc ctggtgctgt ggggcatgct
     8461 ggcctacctc tggggggcgg tcgtgcacct gcgcctgggg gcgtaccggc ccggcctgat
     8521 ctggggcgac tctggagacg gcctgttcgc ggtctgggtc ctgcggcacg gcgcggactg
     8581 gctgcgcggc gcggcgccgc tcgcggacgg ccgtatcttc tggccaagcc acgcgctctc
     8641 cgccttctgg tccgagttat acctgctgat ggcgccggtc ttcatgctgc tggagaaact
     8701 gaccgccagt tccttcgcgg ccgcctacgc cctcggggtt ctgctgtccg tggtggcctt
     8761 ttttgcgtgc ctctttttcg tcgagcaggt gctggccacc gcgcgggacc cggatcgccc
     8821 gccggtcccg ggctgggtgc cgccgctggt cgcctacctg gcttggttct cgatcggcct
     8881 tctccagata ttcaagatcc aggtgctccc ttcgttctgg ctgctggtca tgaccggcgg
     8941 catgctggcc tggcgccgga ccggcctcgc gcgctggatg ctgctcgtct gggcgtcgct
     9001 ggcggccgtc ttcctttcct cttcctactt cgccgccgcg ggcgtgctgc tgttcgccgc
     9061 gtgggcctgg gtcgaatggt tccgcgatcc ccgcgtgtgg tggcgcgtgt tcctcaagct
     9121 ctggcccgcg ctgctcgcgg gcgccgccgc gatgggcgcc gtggcgctga taatgtcgcg
     9181 gccgggcgtg cagcggtatt cgcccgaata catccacagt atggccgtcg gctggctgga
     9241 cctggtccgg ccgcgcgacg gattggtcag cgccgggtgg ctccgcgccc tcaccggcgc
     9301 ggcggaaaac ccgtcgaaca tcggcgcgtt tctcggctgg ggctggatcg tggcggcggc
     9361 ggcctggctg gcccgcgcac gccgcgaagt ctggtctttc ctgaaacagg ccctccgctc
     9421 gcgatggttc gtggcgctgg ccctggtcct gctggcgggg cggctgaaga tccgggaagc
     9481 ccgcgcgctg ctggcgatcc ccggcttcct ggccgtggcg ctcgcgctgc tggtctccgc
     9541 cgcggccgtg ggtcgtcacg gccgggataa gccccgcctt tacgcgatgg gcctcctggc
     9601 cctgttcgcc gtgctcctgt tcggcctgtc cttcggcccg gccgggtggt acgtcggcca
     9661 ccctcttcac ccctccgtgt ggtcggcgct ccgcctgacc attcccggcg tcgccgccat
     9721 gcgggcgatc gaccggatgg ccgtggtcgc ggtggtcttc gcct
//
LOCUS       DAOOTK010000115         9629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4574_length_9629_cov_5.016921, whole genome shotgun sequence.
ACCESSION   DAOOTK010000115 DAOOTK010000000
VERSION     DAOOTK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4574_length_9629_cov_5.016921"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2770
                     /locus_tag="PK634_11450"
     CDS             <1..2770
                     /locus_tag="PK634_11450"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98392.1"
                     /translation="WTNASPTGNYSVTFNAADNDGADAETISIYVNPPAPTGTIYFQG
                     FDGSINDTWGFSGSGSTLAAAARTGGYGRRIGGTDSLTFSNVDLTGHSYVMLNIHCAS
                     TGGVENFDALQVFVALDGGAFPAEPDITVEEGNPTDAVANLTWIYSGIGVAHTTAGTP
                     ATFNGDGTEGYATIRVRIPDGSSSVSVKIAADNSSTSEYYYVDDVEITSGTWQFHDPP
                     VLAAVGSREGAQGASLIFAVGATDPDDDAVSLVASNLPDGAVFVDHGNGSGEFNWAYP
                     AVTGSWATTFHAYNEDGSDSEQIAVFIDPAASSSNVSFTVMAANLSSGTDQAYHEPGI
                     RIFQGLKPDIACLQEFNYASGARALVDTAFGTEFSYYVETDGEAIPNGIVSKYPILSS
                     GQWVDAEISNRDFAWAQIDIPGTQDLYVVSVHLKADSSSASIRNTEAVALKTYITNNW
                     PANAFIAICGDFNTYSRTESCISTLAAVVSDAIQPADQNSDKETNSARDNPYDRVLTS
                     TNLNAKHTATVLGGITFANGLVFDSRVWSTPPDPVLSSDSGEDGMQHMGVMKTFQLEQ
                     ASVPATSAVIYVLAGPNGSISPTNSTVNFGQNKTFTIAAATNYTIADVQVDGASIGTT
                     NAYTFTNVTNHHSIAATFLYNGLSITVIDLVGPDSGSSEENAAAGISISRTNLNATIT
                     AGATQFLLTGWTRTGSSPASGASNMTGQFTMTTNTTVTWLWSTSYWFNSEAGAGGSVN
                     AADRWAALGSNVTITATPSNYYNFSAWSGDAPSGEETNATITVAMNGPRQVTATFAAR
                     LATNDVPEWWLAQYGWTADFDDAAMGDPDGDTALTWQEYVAETIPTNKGSVFQVDARR
                     AAAAGYAVLAWPSAAGRTYDVDYRTNAASGNWEQYQTNIAAIAPLNVYTGAMGADRLF
                     FRVRVRRP"
     gene            2871..3068
                     /locus_tag="PK634_11455"
     CDS             2871..3068
                     /locus_tag="PK634_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98393.1"
                     /translation="MRFFKWGRHEEASDAQYTGVMALKELWGIGEEKKSFKPLTAPVC
                     TDIYHVPTVLNIVPRRRPSRH"
     gene            3107..4393
                     /gene="rsmB"
                     /locus_tag="PK634_11460"
     CDS             3107..4393
                     /gene="rsmB"
                     /locus_tag="PK634_11460"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOW98394.1"
                     /translation="MPESARERLEAARIIAAWLKTAAFPDRLLDRLSSKRSFIMELAY
                     GVIRGWRALDWIRSQLAPRKPRPQLDALALVGLYQMLFMEGVPEYAAVHETVAAARAA
                     GDARGAGFVNALLRRAQTERASLMALLARQPPGIRLSHPDILLDRWTGRHGGDGAARL
                     CEWNNTPPTTTLRVTDRIAGVPAFLAALDRAGVQAAPHPADPGRFVELPHGFRVAALP
                     GYAEGWFAVQDPSTAMAVDLLAPKPGERVLDACAAPGGKAILVSERMERSGLLVAMDS
                     SADRLKRLRENLARFRDDFARVAEGRADDPALLEKGLPPDARGGFDAILVDAPCTNTG
                     VLRRRPDARWRFTEDRLRGAVRKQRALLNALAGVVAPGGRIVYSTCSLEPEENEEQVR
                     SWLKEHPGLSMEEERALRPPGSRTDGAYAARLVLRC"
     gene            4410..5213
                     /locus_tag="PK634_11465"
     CDS             4410..5213
                     /locus_tag="PK634_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOW98395.1"
                     /translation="MTEGYVHGYDPRENVRLQDQASTLVELLHSDTRYPAGARVLEAG
                     CGVGAQTVTLARNSPQALITSVDISATSLAEARRRVGEAGLANVTFLQADLFALPFDT
                     DSFDDLFVCFVLEHLPRPADALRALKRHLRPGGTITAIEGDHGSAYFHPDSEAARRAI
                     QCQVELQRRAGGDAMIGRRLYPLLLEAGFETVRVSPRMVYVDASRPAFVEGFTKKTFT
                     AMIEGVRAPALAAGLLDPPAFDEGIRALYRTAEPDGVFCYTFFKAVARK"
     gene            5252..6448
                     /locus_tag="PK634_11470"
     CDS             5252..6448
                     /locus_tag="PK634_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98396.1"
                     /translation="MKCSGRRRARTGPLVVLLLAGLAVARPAEAAAAARPRLAIWCEF
                     MPYRDVLGTLPLLEKYCCDLLLHVGPGEIGDPDLARVYREAAARKITVAAWFLHPYEE
                     HLYVGEDTIAATRALAFRFLDWAEKERLDARWMVFDCEPSPLLGRELFTHARNFNIPA
                     LARTLRREKNADRFAKSVAELNALIDALHARDVRVMGAANRVFLDFLRYGNTTAQDTL
                     NAPFSMVRWDRISYITYRYHATQPQYVTLVNRYATLARRFHGDRAALDLGLIGDQSGF
                     PEHRERALLFGGSQRFISYLYGMRSVYDLREVIAVALSRGVHRINLYSLEGAVESVAG
                     LENWLKAASEARPARALDRWTPIGSIRAGIFGAALQGLFKAMIGEHPPQDGGGVTLQV
                     DLERNP"
     gene            complement(6414..7886)
                     /gene="gcvPB"
                     /locus_tag="PK634_11475"
     CDS             complement(6414..7886)
                     /gene="gcvPB"
                     /locus_tag="PK634_11475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOW98397.1"
                     /translation="MKLLYEKSVPGRRAVRMPDGAGEPPPRIPAALRRDQPADLPELA
                     EVEVVRHFTALSRLNFSVDTHFYPLGSCTMKYNPKACEAAAALPGLADLHPLWPQLRH
                     GGMLTQGALQLLYETERMLSEITGMAETTLQPLAGAHGELTGVMLMAAYHRDRGNRKT
                     HILVPDSAHGTNPASAAIAGYDVVSVPSDASGRMDLEAFRRAINAETAGVMMTSPSTL
                     GLFVANSRDIADLVHAVDGLMYYDGANLNAILGRCKPAEMGFDICHLNLHKTFATPHG
                     GGGPGSGPVGVVEKLRPFLPISRVVKQPDGTFALDYNLPKSIGYIAPFYGNFGVIVRA
                     YAYLLMIGREGLREVSNMAVLNANYVQEKLRPHFAAVAAGRCMHECVFSGKPLGAHGV
                     HTLDLAKALIDRGVHPPTVYFPLNVPEAIMIEPTETETRETLDRFADAMIELAGLAKT
                     DPEALKRAPVTTPVGRLDEVAAARNTDLVFRGCVPGRPGV"
     gene            complement(7883..9226)
                     /gene="gcvPA"
                     /locus_tag="PK634_11480"
     CDS             complement(7883..9226)
                     /gene="gcvPA"
                     /locus_tag="PK634_11480"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOW98398.1"
                     /translation="MSPTLFNAAGEREQADMLRTIGAKDFDDLFIHVPAEYQAPEFRL
                     PAGRSELEMMQVLRGLAARNSGRLINFCGGGFYDHFIPAAVDALSSRGEFYTAYTPYQ
                     PEAAQGTLQAIYEYQTAIARLTEMDAANASLYDGGTALFEGVMMALRITGRRLVLVDQ
                     GVSPIYRTMLRSYTATLSIEYEEIPLAGGRADRAAFRARLNSDAAAVVLQNPNFFGSV
                     DDFSDLAAAAREAGALTVMSVYPIALGILKTPGAMGADIATGEGQSLGLPLSFGGPYL
                     GFMAVRQAHVRKMPGRIVGAAKDALDRRGFVLTLQAREQHIRREKAMSNICTNEALCA
                     LRAVIYLSLVGKQGLVEVANLCAARAAYARERLLAIPGVKPAFDAPNFNELALALPRD
                     ASEVVSALIEQGIAAGFPVGRYYPGLENVLLLAFTEKRTKEEINILAAKLEAALS"
     gene            9337..>9629
                     /locus_tag="PK634_11485"
     CDS             9337..>9629
                     /locus_tag="PK634_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HOW98399.1"
                     /translation="MSTDQPAVGSIFDLYKIGAGPSSSHSIGTERAARRFLARQPTPP
                     ASVRVTLFGSLAATGRGHLTDQGIRRALDGIPVEIVWDTDAGNLKHPNTIRFDA"
BASE COUNT         1543 a         3421 c         3212 g         1453 t
ORIGIN      
        1 gtggaccaat gcctcgccca ccgggaacta ctcggtgaca ttcaacgccg cagacaacga
       61 cggggcggac gcggagacca tcagcatcta cgtcaatccg ccggccccga cggggaccat
      121 ctacttccag ggcttcgacg gcagcatcaa tgacacctgg ggcttcagcg gcagtgggag
      181 caccctggcg gcggccgcgc gcacgggcgg ctacgggcgc cggatcgggg gcaccgactc
      241 gctcaccttc agcaacgtgg acctgaccgg ccacagctac gtgatgctga acatccactg
      301 cgcgtccacg ggcggcgtgg agaacttcga tgcgttgcag gtcttcgtgg cgctggacgg
      361 gggcgcgttc ccggcggaac ccgacatcac ggtcgaggag ggcaatccca cggacgccgt
      421 ggccaacctg acctggatct actcgggcat cggggtcgcc catacgaccg ccggcacgcc
      481 ggcgacgttc aacggcgacg ggacggaagg ctacgccacg atccgcgtgc ggatccccga
      541 cggctcctcc agcgtgagcg tcaagatcgc cgccgacaac agctccacga gcgagtacta
      601 ctacgtggac gacgtggaga tcaccagcgg cacctggcag ttccatgacc cgcccgtgct
      661 ggccgccgtg ggcagccgcg agggcgccca gggcgcgagc ctgatcttcg ccgtcggcgc
      721 gaccgacccg gacgacgacg cggtctcgct ggtcgccagc aacctgccgg acggcgcggt
      781 gttcgtggat cacggaaacg ggtcgggcga gttcaactgg gcctacccgg ccgtcaccgg
      841 gtcgtgggcg acgaccttcc acgcctacaa cgaggacggg tcggactcgg aacagatcgc
      901 ggtcttcatc gacccggccg cgtcctcttc caacgtctcg ttcacggtca tggccgcgaa
      961 cctctccagc gggaccgacc aggcctacca cgagccgggc atccggatct tccagggcct
     1021 gaagccggac atcgcgtgcc tgcaggaatt caactacgcc agcggcgcac gcgccctcgt
     1081 ggacacggcc ttcggcacgg agttctccta ttacgtggaa accgacgggg aggcgatccc
     1141 caacggcatc gtcagcaagt acccgatcct gtcctcgggc caatgggtgg acgcggagat
     1201 ctcgaaccgc gacttcgcgt gggcccagat cgacatcccc ggcacgcagg acctctatgt
     1261 ggtcagcgtg catctgaagg ccgacagcag ttccgcctcc atccgcaaca ccgaggctgt
     1321 ggccctgaag acctacatca cgaacaactg gcccgccaac gccttcatcg cgatctgcgg
     1381 cgacttcaac acgtacagcc ggacggaaag ctgcatctcc acgctggctg cggtcgtctc
     1441 cgacgccatc cagccggcgg accagaactc cgacaaggag acgaactccg cccgcgataa
     1501 cccgtatgac cgggtcctga ccagcaccaa cctcaacgcg aagcacaccg ccaccgtgct
     1561 gggcggcatc accttcgcga acgggctcgt cttcgacagc cgcgtatggt cgacgccgcc
     1621 cgatcccgtc ttgagctccg actccggcga ggacggcatg cagcacatgg gcgtgatgaa
     1681 gaccttccag ctcgagcagg cgtccgtccc ggcgacctcg gcggtgatct acgtcctggc
     1741 cgggccgaac gggtccatct cgcccaccaa cagcacggtc aacttcggcc agaacaagac
     1801 cttcaccatc gcggccgcga ccaactacac catcgcggac gtgcaggtgg acggcgcctc
     1861 gatcgggacc acgaatgcgt acacgttcac gaacgtcacg aaccaccaca gcatcgcggc
     1921 aacgttcctc tataacgggt tgtcgatcac cgtcatcgac ctggtcggcc cggattcggg
     1981 ctcgtccgag gagaacgccg ccgccgggat ctccatttcc cgcacgaacc tgaacgccac
     2041 gatcacggcc ggcgccacgc agttcctgct gaccggctgg acgcggaccg gctcctcgcc
     2101 ggcctccggc gcctccaaca tgaccggcca gttcaccatg acgacgaaca cgaccgtgac
     2161 ctggctgtgg tccaccagct actggttcaa cagcgaggcc ggcgcgggcg gcagcgtgaa
     2221 cgccgcggac cgctgggccg cgctgggcag caacgtgacg atcacggcga cgccgtcgaa
     2281 ctactataac ttcagcgcgt ggagcggcga cgcgccctcc ggcgaggaaa ccaacgccac
     2341 gatcacggtc gcgatgaacg ggccgcgcca ggtgacggcg accttcgccg cccggctcgc
     2401 cacgaacgac gtgcccgagt ggtggctggc gcagtacggc tggaccgccg atttcgacga
     2461 cgccgcgatg ggcgatccgg acggggacac ggcgctcacg tggcaggaat acgtggccga
     2521 gaccatcccg accaacaagg gttccgtctt ccaggtcgac gcgcgccgcg cggccgcggc
     2581 cggatatgcc gtcctcgcct ggccgagcgc cgcgggccgc acctacgacg tggactatcg
     2641 gaccaacgcc gccagcggaa actgggaaca gtaccagacg aatatcgcgg ccatcgcgcc
     2701 gctgaacgtg tataccggcg ccatgggggc ggatcgcctg ttcttccgcg tccgggtccg
     2761 gcgcccttga ccggcgcgcc ccccgcctga aaaactccac cctatacagg cataaaacgt
     2821 ttaggtatgt ttagcgcctg taaggtcaac ccttcggagg tggaggaggc atgcgcttct
     2881 ttaaatgggg ccggcatgag gaagcgtccg acgcccagta cacgggagtc atggcgctga
     2941 aggagctgtg gggaatcggc gaggaaaaga aatcgttcaa gccgctcacc gcgccggtgt
     3001 gcacggacat ctaccatgtc ccgacggtcc tgaacatcgt tccccgccgg cgcccttcgc
     3061 gtcattgacg ctccggcccg cgtcgctttt gaacacgtcg caccgcatgc ccgaatccgc
     3121 gcgcgagcgg ctcgaggccg cgcgcatcat cgccgcctgg ttgaaaacgg cggcctttcc
     3181 cgaccggctg ctggatcgcc tgtcctcgaa gcgctcattc atcatggaac tggcctacgg
     3241 agtcatccgc ggctggcgcg ccctggactg gatccgctcg caactcgccc cgcgcaaacc
     3301 ccggccgcaa ctggacgcgc tggcgctcgt cgggctgtac cagatgctct tcatggaagg
     3361 cgtgccggaa tacgcggccg tccacgaaac cgtcgcggcg gcccgcgccg ccggggacgc
     3421 ccgtggcgcc gggttcgtca atgccctcct ccgccgcgcc cagacggagc gggcgagcct
     3481 tatggcgctc ctggccaggc agccgcccgg catccgcctc tcgcatccgg atatcctgct
     3541 cgaccgctgg accggccggc acggcggcga cggggccgcc cgactctgcg agtggaacaa
     3601 cacgccgccc accacgaccc ttcgagtcac cgatcggatc gccggggtcc cggccttcct
     3661 ggccgcgctg gatcgcgccg gcgttcaagc ggcgccccac ccggcggatc ccgggcggtt
     3721 cgtggagttg ccgcacggat tccgcgtggc cgcgctgccg ggctacgccg agggctggtt
     3781 cgccgtgcag gatccctcga ccgccatggc cgtggacctg ctcgcgccga aaccgggcga
     3841 acgcgtcctc gacgcctgcg cggcgcccgg cgggaaggcg attctcgtct ccgagcgcat
     3901 ggagcggagc ggcctgctcg tggccatgga ttcctccgcc gatcgcctga aacgcctccg
     3961 cgaaaacctg gctcgcttcc gcgacgactt cgcgcgcgtg gcggaaggcc gcgcggacga
     4021 tcccgccctg ctcgaaaagg gcctgccgcc ggacgcgcgc ggcgggttcg acgccatcct
     4081 cgtggacgcg ccctgcacga acacgggcgt gctgcgccgc cgccccgatg cccgctggcg
     4141 gttcaccgag gaccggctgc ggggcgcggt tcgcaagcag cgggcgctgc tcaacgcgct
     4201 ggccggcgtc gttgcgcctg gaggccggat cgtgtatagc acatgcagcc tcgagcccga
     4261 ggagaacgag gagcaggtcc ggtcatggct gaaggagcat cccggtttat ccatggaaga
     4321 ggagcgcgcc ctacggccgc ccggctcccg gaccgacggc gcctacgcgg cgcggctggt
     4381 cctccgctgc tgatgtcgcg gaggcgccca tgacggaagg atatgtccac ggctacgacc
     4441 cgcgcgaaaa cgtccggctc caggaccagg cctcgacgct ggtcgaactg ctgcactccg
     4501 atacgcgcta cccggccggc gcgcgcgtgc tggaggccgg ctgcggggtc ggcgcgcaga
     4561 ccgtcaccct ggcccggaac agcccgcagg ccctgatcac ctcggtggac atctcggcga
     4621 cctcgctcgc cgaggcccgc cggcgggtcg gggaggcggg gctggccaat gtgactttcc
     4681 tccaggccga cctcttcgcc cttcccttcg ataccgactc cttcgacgac ctgttcgtgt
     4741 gtttcgtgct ggagcacctg ccgcggcccg ccgacgcgct gcgggccctg aagcgccacc
     4801 tgcggcccgg cggcaccatc acggcgatcg agggcgacca cggctcggcg tatttccacc
     4861 ccgacagcga ggccgctcgc cgggcgatcc aatgccaggt cgaactgcag cggcgggcgg
     4921 gcggcgacgc catgatcggc cggcggttat atcccctgct cctcgaggcc ggctttgaaa
     4981 cggtccgcgt ctcgccccgg atggtctacg tggatgccag ccggccggcc ttcgtcgagg
     5041 ggttcacaaa aaagactttc accgccatga tcgagggggt gcgggcgccg gccctggccg
     5101 ccgggctcct cgatccgccc gcattcgacg agggcatccg cgccctctac cggaccgcgg
     5161 aaccggacgg ggtcttctgc tacaccttct tcaaagccgt cgccaggaag tgattgcggg
     5221 gcctccgctt tgtgactatt ttccgccaac catgaaatgc tccggcagac ggcgcgcgcg
     5281 cacgggacct ctggttgtcc tgctgctggc cggtctcgcg gtggcgcggc ccgccgaggc
     5341 ggccgcggcc gcccgccccc gcctggcgat ctggtgcgag ttcatgccct accgggacgt
     5401 gctgggcacg ctgccgctgc tcgaaaaata ctgctgcgac ctgctgctcc acgtcgggcc
     5461 gggcgagatc ggcgatcccg acctcgcgcg cgtctaccgc gaggccgcgg cgcggaagat
     5521 caccgtcgcc gcgtggttcc tgcacccgta cgaggagcac ctgtacgtcg gagaggacac
     5581 gatcgcggca acccgcgcgc tcgcgttccg gtttctggac tgggcggaga aggagcgcct
     5641 ggacgcccgc tggatggtgt tcgactgcga gcccagcccc ctgctcggcc gggagctttt
     5701 cacgcacgcg cggaacttca acatccccgc cctcgcgcgc acgctgcgcc gggagaagaa
     5761 cgccgaccgc ttcgccaaat ccgtcgccga gctgaacgcg ctgatcgatg ccctgcacgc
     5821 ccgcgacgtc cgggtgatgg gcgcggcgaa ccgcgtcttt cttgatttcc tgcgctacgg
     5881 gaacaccacg gcgcaggata cgctcaacgc gccgttcagc atggtgcggt gggaccggat
     5941 ctcgtacatc acctatcgct accacgccac gcagccgcag tacgtgaccc tggtcaaccg
     6001 ctacgcgacg ctggcccgcc ggttccacgg cgaccgcgcc gcgctcgatc tcgggctgat
     6061 cggcgaccag agcggcttcc ccgagcaccg cgagcgggcg ctgctcttcg gcggcagcca
     6121 gcgcttcatc tcgtacctgt acgggatgcg ctcggtgtac gacctgcgcg aggtcatcgc
     6181 cgtcgccttg agccgcgggg tgcaccggat caacctgtac tcgctggagg gcgcggtgga
     6241 gtccgtcgcc gggctggaga actggctcaa ggccgcctcg gaagcccggc cggcccgcgc
     6301 gctggaccgc tggacgccga tcgggtccat ccgcgcagga attttcggcg ccgcgctcca
     6361 gggcctgttc aaggcgatga tcggggagca tccgcctcaa gacggcggcg gcgtcacact
     6421 ccaggtcgac ctggaacgca acccctgaac accaggtccg tgttccgcgc ggcggcgacc
     6481 tcgtccagcc ggccgaccgg cgtggtgacc ggggctcgct tcaaggcttc cggatcggtc
     6541 ttcgccaggc cggccagctc gatcatcgcg tccgcgaacc ggtccagggt ctcacgcgtc
     6601 tcggtctccg tcggctcgat catgatcgct tcgggaacgt tgagcgggaa gtacacggtc
     6661 ggcgggtgga cgccgcggtc gatcagggcc ttggccaggt ccagcgtgtg cacgccgtgc
     6721 gcgccgagcg gcttgccgct gaacacgcac tcgtgcatgc agcggccggc ggcgacggcc
     6781 gcgaagtgcg gacgcagctt ttcctgcacg tagttcgcgt tcagcacggc catgttgctg
     6841 acctcgcgca ggccctcgcg gccgatcatc agcaggtagg cgtaggcccg gacgatcacg
     6901 ccgaaattgc cgtagaaggg cgcgatgtag ccgatgctct tcggaaggtt gtagtccagc
     6961 gcgaaggtgc cgtcgggctg cttcacgacg cgggagatcg gcaggaaggg ccgcagcttc
     7021 tccacgacgc ccaccgggcc cgagcccggg ccgccgccgc cgtgcggggt ggcgaaggtc
     7081 ttgtgcaggt tcaggtggca gatgtcgaag cccatctccg ccggcttgca gcggccgagg
     7141 atcgcgttga ggttcgcccc gtcgtagtac atcagcccgt ccacggcgtg caccaggtcc
     7201 gcaatatcgc ggctgttggc gacgaacagg ccgagcgtcg aggggctggt catcatcacg
     7261 cccgcggtct ccgcgttgat ggcccggcgg aaggcctcga ggtccatccg gccggaggcg
     7321 tccgagggca cgctgacgac gtcgtacccg gcgatcgccg cgctggccgg gttggtgccg
     7381 tgcgcggaat cgggcacgag gatgtgcgtc ttgcggttcc cgcggtcgcg gtggtaggcg
     7441 gccatcagca tgacgccggt cagctcgccg tgcgcgcccg cgagcggctg cagcgtggtc
     7501 tcggccatgc cggtgatctc ggagagcatc cgctcggttt cgtacagaag ctgcagcgcg
     7561 ccctgggtca gcatcccgcc gtgccggagt tgcggccaga gcggatgcag gtccgccagg
     7621 cccggcagcg cggcggccgc ctcgcaagct ttggggttgt acttcatcgt gcaggagccg
     7681 agggggtaaa aatgcgtgtc cacggagaag ttgaggcggg agagcgcggt gaaatggcgc
     7741 accacctcga cctccgccag ctccggcagg tccgccggct gatcgcgccg cagcgccgcg
     7801 ggaatccgcg ggggcggctc gcccgcgccg tccggcatcc gcacggcccg gcggccgggc
     7861 acgctcttct cgtacagtag cttcatgaca gcgccgcctc cagcttggcg gccaggatat
     7921 tgatttcctc cttcgtccgc ttctcggtga aggccagcag cagcacgttc tccaggccgg
     7981 gataataccg accgaccggg aaaccggcgg cgatgccctg ctcgatgagc gcgctgacga
     8041 cctccgaggc gtcccgcggc agagcgaggg ccaactcgtt aaagttcggc gcgtcgaagg
     8101 ccggcttcac gcccgggatc gccagcagcc gctcccgcgc gtacgcggcg cgcgcggcgc
     8161 acaggttggc gacctcgacg agcccctgtt tgccgacgag cgagaggtag atcaccgcgc
     8221 gcagggcgca gagcgcctcg ttggtgcaga tgttggacat cgccttctcg cggcggatgt
     8281 gctgctctcg cgcctgcagg gtcagcacga acccgcgccg gtccagcgcg tccttcgccg
     8341 cgccgacgat ccggccgggc atcttgcgca cgtgggcctg gcggacggcc atgaacccga
     8401 ggtacggccc gccgaacgag agcggcaggc cgaggctctg cccctcgccc gtggcgatgt
     8461 ccgcgcccat cgcgccgggc gttttcagga ttccgagggc gatcgggtac acggacatca
     8521 ccgtcagcgc gccggcctcc cgggcggcgg cggccaggtc ggaaaaatcg tccacgctgc
     8581 cgaaaaaatt cgggttctgc aacacgaccg cggcggcgtc gctattcaag cgggcacgga
     8641 aggcggctcg atccgcccgc ccgccggcca acgggatctc ctcgtactcg atggaaaggg
     8701 ttgccgtgta actgcggagc atcgtccggt agatcgggct gacgccctgg tccacgagca
     8761 caagccgccg gcccgtgatg cgcagggcca tcatcacgcc ctcgaacagc gccgtgccgc
     8821 cgtcgtagag cgaggcgttg gccgcgtcca tttccgtcag ccgcgcgatg gcggtctggt
     8881 attcgtagat cgcctggagc gtgccctgcg ccgcctcggg ctggtacggc gtgtaggccg
     8941 tgtagaactc gccccgggac gaaagggcgt ccacggcggc cgggatgaag tggtcgtaga
     9001 atccgccgcc gcaaaaattg atcagccgtc ccgaattccg cgccgcgagg ccccgcagga
     9061 cctgcatcat ctccagttcg gaccggccgg ccggcaaacg gaactcgggc gcctggtatt
     9121 cggccgggac gtggatgaac agatcatcga aatccttcgc gccgatcgtg cgaagcatat
     9181 cggcctgttc cctctcgccc gccgcgttga agagagttgg agacatcggg tcgctacctt
     9241 actcccgcgg tccgcggcgc gcaagtttcc tgccgtccgg cttgatgccg ccgccacgcg
     9301 cgggtaggat tccggggccg catccggaga aatgacatga gcacggacca gcccgcggtg
     9361 ggctcgattt tcgacctcta caagattggc gccggcccct cgagttcgca cagcatcggg
     9421 accgagagag ccgcccgccg cttcctcgcc cggcagccca cgccgcccgc ctcggtccgc
     9481 gtgacgctct tcggcagcct cgcggccacc gggcgcggac acctcaccga ccagggcatc
     9541 cggcgggcgc tggacggaat acccgtcgag atcgtctggg ataccgacgc cggcaacttg
     9601 aagcatccca acacgatccg gttcgacgc
//
LOCUS       DAOOTK010000116         9528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4623_length_9528_cov_5.656497, whole genome shotgun sequence.
ACCESSION   DAOOTK010000116 DAOOTK010000000
VERSION     DAOOTK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4623_length_9528_cov_5.656497"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PK634_11490"
     CDS             <1..694
                     /locus_tag="PK634_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOW98400.1"
                     /translation="RHMLQTTHQLKAPFAAIQANTQLMLDGYCGELPAEARDVAQRIS
                     ARCRRLTRDIQDMLQLANLRSEGQKPPAPQEVDLQEAWRYVIHQLEPLASEREVRLDI
                     ALVPARVPGIPDHLKMLFINLLSNAIMYSRRGGRVRVEAGTLAGGGARVRVADEGIGI
                     APEKLPRIFDEYYRTEEAVQFNKESSGLGLAIVRHVAREHGLRLKVASRPDHGTAFTL
                     DFPSVAQSPKRN"
     gene            716..1105
                     /locus_tag="PK634_11495"
     CDS             716..1105
                     /locus_tag="PK634_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW98401.1"
                     /translation="MAYILIVDDDEDFAAASAAALRKAGHEVKVETRASRALQVLEAR
                     PPDLAVLDVMFPENESAGFDLARQMRQHPALKATPILMLTAINMKFPLGFGSADKDEE
                     WLPVTDFVEKPVDFDVLCRKVAALLAT"
     gene            1292..1609
                     /locus_tag="PK634_11500"
     CDS             1292..1609
                     /locus_tag="PK634_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOW98402.1"
                     /translation="MNYRQAKTRAEILKAMAHPVRVLIADTLTRGDQCVRELNRLARI
                     NQSNVSRHLAVLKKAGIISDRRIGMKVLYHLETPCILRAFDCAAEVVQADARRRSQYL
                     KAV"
     gene            1611..2990
                     /locus_tag="PK634_11505"
     CDS             1611..2990
                     /locus_tag="PK634_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOW98403.1"
                     /translation="MGRERKIALGLAAGFLAAYFLPVEAARFQGAVTESLRLVRWYAR
                     EHVLLCLVPAFFIAGAIGVFVRQDAVMKYFGPKAHKALAYGVASVSGTILAVCSCTVL
                     PLFGGIYLRGAGLGPAIAFLYSGPAINVLAIVLTARVLGWQLGLARSVGAVSFSVVIG
                     LLMHLIFLKEERAKAAQVQDVYMGDDDGKDHPLWQVALYFLSMVGILVFANWGKPMEP
                     AGVWFAIYKTKWIVTALFAVLLGLCLWRFFRVRLAYIALAAVPPLLLAGAFPGHPVVA
                     FAAGSAGLTVLTFVGGADLKDWRDQTWGFAKQILPLLFMGVLAAGFFLGSPGSKDAGI
                     VPNVWIQALVGDSPSSLLSIFGKPEGTHIQWLETIWPLWTNLFASITGALMYFATLTE
                     VPILRGLLDSGMGQGPALALLLAGPALSLPSMLVINSILGPRKTLTFVGLVVAMATIT
                     GMIFGGLVT"
     gene            3000..3233
                     /locus_tag="PK634_11510"
     CDS             3000..3233
                     /locus_tag="PK634_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOW98404.1"
                     /translation="MKKIQILGTGCPKCKLLAANAADAVKALAIEAQIEKVEKIGEIM
                     KFGVMTTPALVVDGVVKSAGKVLSADDIKKHLA"
     gene            3243..3389
                     /locus_tag="PK634_11515"
                     /pseudo
     CDS             3243..3389
                     /locus_tag="PK634_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
     gene            3660..3995
                     /locus_tag="PK634_11520"
     CDS             3660..3995
                     /locus_tag="PK634_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOW98405.1"
                     /translation="MEASAAAASERPAPLPRLVDLGADKCVPCKAMAPILEELKKTYA
                     GQLQVDFIDVWKNPGAGDQYGLRVIPTQIFFSADGKELFRHEGFFSREDILAKWAEFG
                     VAFSDTGAP"
     gene            3995..4702
                     /locus_tag="PK634_11525"
     CDS             3995..4702
                     /locus_tag="PK634_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOW98406.1"
                     /translation="MGWIQAFFSSLSHAIEGNPAVAVAAALAWGILSILLSPCHLASI
                     PLIIGFIDEQGSISTRRAFWIALLFSAGILVTIALIGVATALAGRMLGDVGEYGNYVV
                     AVIFFIVGLHLLDVIPLNFGGPGRVNMKRRGLLASFILGLVFGVALGPCTFAFMAPML
                     AITFKLSAANGLYGAFLLVMYGVGHCGVIVAAGTSYEWIEHYLKWSEASRGALRIKKA
                     CGVLVLLGGLWLLYTAP"
     gene            4712..5152
                     /locus_tag="PK634_11530"
     CDS             4712..5152
                     /locus_tag="PK634_11530"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOW98407.1"
                     /translation="MNSLRSFRTVLCAGLMGVVLASFSEAAEAPAKKPLEKTVFYFHR
                     TARCPTCLKLESWTREAVQARSTGEQSKTLVFKPVNLDAPENKHFEEDFQLTFSTAVA
                     AEMQEDGRAARWKNLDRIWEYSADEAAFRKYVDGELGAFFNAKP"
     gene            5149..5580
                     /locus_tag="PK634_11535"
     CDS             5149..5580
                     /locus_tag="PK634_11535"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOW98408.1"
                     /translation="MNRLKILFLCTGNSCRSQMAEGWARHLKGDVIEAYSAGIETHGL
                     NPLAVKVMAEAGVDISKQRSKRVAELKGIEFDHVVTVCDHAHESCPLFPGRTKVTHRG
                     FDDPPRLAKDVRTDEEALRPYRRVRDEIRAFVEMLPEALKT"
     gene            5577..6791
                     /gene="arsB"
                     /locus_tag="PK634_11540"
     CDS             5577..6791
                     /gene="arsB"
                     /locus_tag="PK634_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOW98409.1"
                     /translation="MSGNDRKLTNVFERYLTVWVALCIIGGILLGKVAPGLAKTLDGM
                     AIRVSGAPVVSIPIAICLFFMMYPIMVKIDFASVIRAGKSGKPVWLTLFVNWAIKPFT
                     MYGIALLFLGVLFRGFIGADAVDLVKMPFGLDLPVGASHGAGTVVLVEGVKMLQVPLW
                     RSYLAGCILLGVAPCTAMVLVWGYLARGNDGLTLVMVAINSLTMLLLYGILGGFLLGV
                     GRLPVPWQALLLSIGIYVALPLVAGYFTRRWILAAKGETWFKEKFLHVLTPITIIALL
                     TTLVLLFSFKGEVILSTPLTILWIAIPLFIQTLLIFGLGYGAARALKLSYEDAAPAAM
                     IGASNHFEVAIATATMLFGLSSGAALATVVGVLIEVPVMLGLVKICLRTRGWFPRPSA
                     VVSPPPRLPAAP"
     gene            6854..7225
                     /locus_tag="PK634_11545"
     CDS             6854..7225
                     /locus_tag="PK634_11545"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOW98410.1"
                     /translation="MDYVNRGYWNAVMSASLCKFLILRVISERPRHGYGIVWRVAELT
                     KHLCAPTQGAVYPALRDFEKAGCVASRIERRGRRERKVYRLTARGREALQAAAEVWHH
                     GLQRLRTIVRPAVTSRMKVGP"
     gene            7329..8156
                     /gene="arsM"
                     /locus_tag="PK634_11550"
     CDS             7329..8156
                     /gene="arsM"
                     /locus_tag="PK634_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOW98411.1"
                     /translation="MKKDVHESVRQGYAAIAAGGGSCCGPAKSCGGGGAADVARTVGY
                     SDAELATLPDGANMGLSCGNPAALAGLKPGEAVLDLGAGGGFDVFLVAQKVGPAGRAI
                     GVDMTPEMVGKARRNTIVFREKTGLDNVEFRLGEIEHLPVADASVDVVISNCVVNLSP
                     DKPQVWREACRVLKPGGRVAVSDLALLQPLPDAVRESVEALVGCVAGAVLIRDTEAMM
                     RQAGFADIVLESKPDYIDAMSRWSDPLYRAIIERLPPGARLADFVTSLSISARKPRP"
     gene            complement(8128..9063)
                     /locus_tag="PK634_11555"
     CDS             complement(8128..9063)
                     /locus_tag="PK634_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOW98412.1"
                     /translation="MINSRIVLALAATWMTAGWPAAAPAWGPEGHTIVADIAVRHLTP
                     RARERMRAILGKKQLGDYEVSSWPDIIRGDKEYDKRYPKNNRWHYVDFNVFDHADELK
                     LPEDEQDIVSQITRWHGELARPKLSRARRRAAVSFLVHFVGDLHQPLHCGYRYNDAGG
                     NFIPIRSFEGEHFTLNDSEPMDHPLNLHSVWDECLVYELMAGRDPDAVARQLLAEITP
                     EQAQSWAAGAPLDWAFESFKLAHDRAYHFADGSDIPFTWSRPGTDLTRENYIDAAVPV
                     AREQLEKAGIRLAHLLNTALDPAAEPATGGASGRK"
     gene            9175..>9528
                     /locus_tag="PK634_11560"
     CDS             9175..>9528
                     /locus_tag="PK634_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOW98413.1"
                     /translation="MNWKDFFDRLGMDGTRWQWRIMRWQQRMAERGAERAAPENRAAS
                     RYKFCETCGAILPREEKVCPRCQAPAPGWRSQYIKRRLALVLPGWVPVTGVLLAANGL
                     VMLAAMLFFGLGALFQ"
BASE COUNT         1527 a         2936 c         3276 g         1789 t
ORIGIN      
        1 gcggcacatg ctgcagacaa cgcatcagct caaggcgccg ttcgccgcga tccaggcgaa
       61 cacgcagctc atgctggacg gctactgcgg ggaactgccc gccgaggccc gcgacgtggc
      121 gcagcgtatc tccgcgcgct gccgccggct gacccgggac atccaggata tgctgcagtt
      181 ggccaacctc cggtccgagg gccagaagcc gccggcgccg caggaggtgg atctccagga
      241 ggcgtggcgg tacgtcattc accagctgga gccgctggcg tccgagcgcg aggtgcgcct
      301 ggacatcgcg ctggtcccgg cccgggttcc cgggatcccc gatcacctga aaatgctgtt
      361 tatcaacctg ctgtcgaacg ctattatgta ttcccggcgc ggggggcgcg tgcgggtgga
      421 ggcgggaact ctcgctggcg gcggggcgcg cgtgcgggtg gcggacgagg gcatcggcat
      481 cgcgcccgaa aagctgccgc gcatattcga cgagtactac cggacggagg aagccgtgca
      541 gttcaacaag gagtcctcgg gactgggatt ggccatcgtg cggcacgtcg cgcgggagca
      601 cggcctccgc ctgaaggtgg ccagccggcc ggatcacggc acggcgttta ctttggactt
      661 tccttccgtc gcgcaatcgc cgaaaagaaa ctaggaaacg ggataggaga ctccgatggc
      721 ctacatactg atcgtggacg acgacgagga tttcgcggcc gcttcggcgg cggctctccg
      781 caaggcgggg cacgaggtga aggtggagac gcgcgccagc cgcgccctgc aggtcctcga
      841 agcgcggccg ccggacctgg cggtcctcga cgtcatgttc ccggagaacg agtccgccgg
      901 gttcgatctg gcccggcaga tgcgtcagca tcccgccctg aaggccacgc cgatcctgat
      961 gctgacggcc atcaacatga agttcccgct cggtttcggt tcggcggaca aggacgagga
     1021 gtggctgccc gtgaccgatt tcgtcgagaa gccggtggat ttcgacgtcc tgtgccgcaa
     1081 ggtggccgcc ctgctggcca cgtagttgct gaagcgcggc ctgtccgaaa aacccggcgc
     1141 gccgggcgcc cgcggggctt gggcttgatc cttccgcccg cggccgcgtg agcccgtccg
     1201 cgtgcccctg cctcgagtgt cccgctccat gctggccgac ggttccgcga agctcttgac
     1261 cttatgcgca tatgcgcata aggtaggtgt catgaactac cggcaagcca agacaagagc
     1321 ggaaatcctg aaggccatgg cgcatcccgt gcgcgtgctg atcgcggaca cgttgacgcg
     1381 gggcgaccag tgcgtccgcg aactgaaccg cctggcgcgg atcaaccagt ccaatgtctc
     1441 ccgtcacctg gcggtgctca agaaggccgg gatcatctcc gaccggcgca tcggcatgaa
     1501 ggtgctctac cacctcgaaa cgccgtgcat tctgagggcg ttcgactgcg ccgcggaggt
     1561 ggtccaggcg gatgcccggc ggcggagcca gtacttgaaa gcggtgtgag gtgggccggg
     1621 agcggaagat agccctcggg ctggcggccg gcttcctcgc ggcgtacttc ctgccggtcg
     1681 aggccgcccg gttccagggc gccgtgaccg aatcgctgcg gctcgtgcgc tggtacgccc
     1741 gggagcatgt cctgctatgc ctcgtgcccg ccttcttcat cgccggcgcc atcggcgtgt
     1801 ttgtccggca ggacgccgtg atgaaatact tcgggccgaa ggcgcacaag gcgctggcct
     1861 acggcgtggc ctcggtgtcc ggcacgatcc tcgccgtatg ctcctgcacc gtgcttcccc
     1921 tcttcggcgg catctacctg cgcggcgcgg gactcggccc ggcgatcgcg ttcctctact
     1981 ccggcccggc gatcaacgtc ctggcgatcg tgctgacggc ccgcgtgctg ggctggcaac
     2041 tcgggctggc gcggtcggtc ggcgccgttt cgttcagcgt ggttatcgga ttgctgatgc
     2101 atctgatctt tctcaaggaa gagcgcgcca aggccgcgca ggttcaggac gtgtacatgg
     2161 gcgatgacga cggcaaggac catccgctct ggcaggtcgc gctctacttc ctctcgatgg
     2221 tcggcatcct cgtcttcgcg aactggggca agccgatgga gccggccggc gtctggttcg
     2281 cgatctacaa gaccaagtgg atcgtcacgg cgctcttcgc cgttctgctg ggtctctgcc
     2341 tctggcggtt cttcagggtg cggcttgcct atatcgcgct ggcggccgtt ccgcccctcc
     2401 tattggcagg ggccttcccc ggccatcccg tggtcgcctt cgcggcgggc tcggccggat
     2461 tgaccgtgct gacgtttgtc ggcggagctg atctcaagga ctggcgcgac cagacgtggg
     2521 gcttcgcgaa gcagattctg cccctgctgt tcatgggtgt gctggccgcg ggcttcttcc
     2581 tcggcagtcc gggatccaaa gacgcgggca ttgtgccgaa tgtctggatt caagcactgg
     2641 ttggggattc gccgtcatcc ctcctttcaa tctttggaaa accagagggc acgcacatcc
     2701 aatggttgga aacgatctgg ccgctctgga ccaacctctt cgcctcgatc accggcgcgc
     2761 tgatgtactt cgccacgctg accgaggttc cgatcctgcg cggcctgctc gacagcggca
     2821 tggggcaggg gccggccctg gcgctcctgc ttgccgggcc agcgctttcg ctgccgagta
     2881 tgttggtgat caactccatt ctcggtccac ggaagacgtt gacattcgtc ggcctggtgg
     2941 tggccatggc gacgatcacc gggatgattt tcggcgggct tgttacatag gagaccggca
     3001 tgaagaagat tcagattctc ggcacgggct gtccgaagtg caaactgctc gcggccaacg
     3061 cggcggatgc cgtgaaggcc ctggccatcg aagcgcagat cgagaaggtc gaaaaaatag
     3121 gggagatcat gaagttcggc gtgatgacca cgccggccct tgtcgtggat ggcgtggtga
     3181 aaagcgccgg caaggtgttg agcgcggacg atatcaagaa gcatttggcc taggagacgg
     3241 tcatgaccga gaaacaagca ggcgcttgtg gaggagcgcc caaactgatc ttcccctgtt
     3301 ccggaggggc ggatgtcggc gccttggcgg accaggccgc ccgacgatta acgagggatg
     3361 gattcggcac gatgtactgc ctggccggct gatgtggtcg ccccatggta aaggtttata
     3421 cgacaccgcc gccggcctgt tcgtgccggg gtgaagaggg caatgacctt gtaccgttgc
     3481 tggtggtcgc agttctcttg atcggcgcgc gacttgtgaa tggatggctg aagaagaaag
     3541 gaaggcgtca cgtgaaaaat ttgaagcagg tggtgatcgt gggcgccctg gttgctgccg
     3601 tgggcggagt attcgccctg aaacagcggc agtcgagacc tgtcgttgcg tccgccgagg
     3661 tggaggcgtc ggctgccgcg gccagcgaaa ggcccgcgcc gctccctcgc ctcgtcgatc
     3721 tcggcgcgga taagtgcgtt ccctgcaagg cgatggcgcc tatcctggag gagctgaaaa
     3781 agacctatgc cggtcagtta caggtggact ttattgatgt ttggaaaaat cccggggcgg
     3841 gcgaccagta cggtctgcgt gtaattccaa cgcagatttt cttctccgcc gacggcaagg
     3901 agttgttccg gcatgaaggc ttcttctcgc gggaggatat tctcgcgaag tgggcggaat
     3961 tcggggtcgc gttcagcgac acgggggcgc cgtaatgggg tggatacagg cattcttctc
     4021 ctcgctttcg cacgccattg aaggcaaccc ggccgtggcc gttgcagcgg cgctggcctg
     4081 gggcatcttg agcatcctct tgagcccctg ccacctggcc agcatcccgc tgatcatcgg
     4141 gttcattgac gagcagggct ccatcagcac caggcgggcg ttctggatcg ccctgttgtt
     4201 ctcggccggc atcctggtca ccatcgcgct gatcggcgtg gcgacggcgc tggcgggacg
     4261 gatgctgggg gacgtgggcg agtacggcaa ctacgtggtc gccgtgatct tctttatcgt
     4321 cggtctgcac ctgctggatg tcatccccct gaacttcggc ggaccgggcc gggtgaacat
     4381 gaagcgcaga ggcctgctgg cgtcgttcat cctgggcctg gtcttcggcg tggcgctcgg
     4441 gccgtgcacc ttcgccttca tggcccccat gctggccatc accttcaagc tctcggccgc
     4501 gaacgggctg tacggcgcct ttctgctggt gatgtatggc gtcgggcatt gcggcgtgat
     4561 cgtggcggcc gggacgtcgt acgaatggat cgagcactat ctgaagtgga gcgaggcctc
     4621 gaggggcgcg ctgcggatca agaaggcctg cggcgtgctg gtgcttctcg gcggtctttg
     4681 gcttctgtac accgcaccct aggagaagga catgaactca ctgcgatcgt tccgaaccgt
     4741 gttgtgcgcg ggcctgatgg gcgtggttct ggcctcgttc tctgaagccg ccgaggcccc
     4801 ggcgaaaaag ccgctggaaa agacggtgtt ctatttccac cggacggccc gctgcccgac
     4861 gtgcctgaaa ctggagtcct ggacccgcga agcggtgcag gcccggtcta ccggggagca
     4921 atcgaagact ctcgtcttca aaccggtaaa cctcgatgcg cctgaaaaca agcacttcga
     4981 agaggatttt cagttgacgt tcagcaccgc ggtcgcggcg gaaatgcagg aagacggccg
     5041 ggcggcccgg tggaaaaacc tcgaccgcat ctgggaatat tccgcggacg aggcggcctt
     5101 caggaagtat gtggacggcg aactcggcgc gtttttcaac gccaagccat gaacaggctg
     5161 aagatactct ttctgtgcac cggcaactcc tgtcggagcc agatggccga gggctgggcg
     5221 cgccatctga agggcgacgt gatcgaagcg tactccgcgg ggatcgagac ccatggactg
     5281 aatcccctgg ccgtgaaggt catggccgag gcgggggtgg acatttccaa acagcggtcc
     5341 aagcgcgtgg ccgagttgaa gggcatcgag ttcgatcatg tcgtgacggt ctgcgatcac
     5401 gcgcacgaat cctgcccgct ctttcccggc cggacgaagg tcacgcaccg cggattcgac
     5461 gatcctccgc gcctggcgaa ggacgtccgg acggacgagg aggccctgag gccttaccgg
     5521 cgggtgcggg atgagatcag ggcctttgtg gaaatgctgc cggaggcgtt aaagacatga
     5581 gcggcaatga tcgcaaattg acaaatgtgt tcgagcgtta cctgaccgtt tgggtcgcct
     5641 tgtgcattat cggcggcatt ctattgggca aagtcgcgcc gggcctcgcg aagacactcg
     5701 acggcatggc gatccgcgtc agcggcgccc ccgtggtgtc catcccgatt gccatctgcc
     5761 tgttcttcat gatgtatccg atcatggtga agatcgactt cgcgtcggtg atccgggccg
     5821 ggaagagcgg aaagcccgtg tggctgacgc tgttcgtgaa ctgggcgatc aagccgttca
     5881 cgatgtacgg catcgcgctc ctgttcctcg gcgttctgtt ccgcgggttc atcggtgccg
     5941 acgctgtgga cctggtgaag atgccgttcg gtctcgacct gcccgtcggc gcatcgcacg
     6001 gcgcgggcac ggtcgtcctg gtcgagggcg tcaagatgct tcaggttccg ctgtggcgca
     6061 gttacctggc cggctgcatc ctgctcggcg ttgcgccgtg cacggcgatg gtcctggtct
     6121 ggggctacct cgcgcgcggg aacgacgggc tgacgctggt aatggtggcg atcaactcgc
     6181 tgacgatgct cctgctctac ggcatcctgg gcggcttcct gctgggggtc ggccggctgc
     6241 cggtgccctg gcaggcgctg ctgctctcca tcggcatcta cgtcgccctg ccgcttgtcg
     6301 ccggctactt cacccgccgg tggatcctcg cggcgaaagg ggagacgtgg ttcaaggaga
     6361 aattcctgca cgtcctgacg ccgatcacga tcatcgccct gctgaccacg ctggtcctcc
     6421 tgttcagctt caagggcgag gtgatcctgt cgacccccct gacgattcta tggattgcga
     6481 tcccgctgtt catccagacg ctgctgatct tcgggctcgg ctatggcgcg gcccgcgcgc
     6541 tgaagctgtc ctacgaggat gccgcgccgg cggcgatgat cggcgcatcg aaccatttcg
     6601 aggtggcgat cgccacggcg acaatgctct tcggcctgtc ctccggcgcg gccctcgcga
     6661 ccgtcgtcgg cgtactgatc gaggtgccgg tgatgctcgg gctggtgaag atctgcctgc
     6721 ggacgcgagg ctggttcccg cgtccctccg ccgtcgtttc tcccccgccc cggttgcccg
     6781 cggccccttg acggcctcgc ggcccggcgc caggatgata cctagagggt ctaggcatca
     6841 tggaggagtg atcatggact atgtcaatcg cgggtactgg aacgcggtga tgagtgcgag
     6901 cctttgcaag ttcctcattc tgcgggtgat cagtgaaagg ccgcggcacg gatatggaat
     6961 cgtctggcgg gtggccgagt tgacgaaaca cttgtgcgcg ccgacgcaag gggccgttta
     7021 tcccgcgctc cgcgacttcg agaaggcggg ctgcgtggcc agccggatcg agcggcgggg
     7081 gcggagggag cgaaaggtgt accggctcac cgcccggggc cgggaagccc tccaggcggc
     7141 cgcggaggtc tggcatcacg gcctgcagcg cctgcggacg atagtgcgtc ccgccgtcac
     7201 ctcccggatg aaagtcggcc catgatacct agagggtcta ggtatcatgg gcgggcgcga
     7261 cacgatgacg cggcgcgagg gggcgagggg aaggcggagc gatcgttgcc atgttacggg
     7321 agatcatcat gaaaaaggat gttcatgaat cggtccgcca gggttatgcg gccatcgccg
     7381 cgggcggcgg atcgtgctgc gggccggcga agagctgcgg cgggggcggg gccgccgacg
     7441 tggcccgaac cgtgggctat tccgacgcgg aactggcgac gctgccggac ggcgcgaaca
     7501 tggggctttc gtgcgggaac ccggcggcgc tggcgggatt gaagccgggc gaggcggtcc
     7561 tggacctcgg cgccggcggc gggtttgacg tctttctcgt cgcgcaaaag gtgggcccgg
     7621 ccgggcgagc gatcggcgtg gacatgacgc cggaaatggt cggcaaggcg cggcggaata
     7681 cgattgtctt ccgggaaaaa accggcctgg acaacgtgga gttccgcctg ggcgagatcg
     7741 agcatcttcc cgtggcggac gcgagcgtgg atgtggtgat ctccaactgc gtggtcaacc
     7801 tttcgcccga caagccgcag gtctggcgcg aggcctgccg cgtgctgaag cccggcggcc
     7861 gcgtcgcggt ttccgacctg gccttgctcc aacccctgcc ggacgcggtg cgggaatccg
     7921 tagaggccct ggtcggttgc gtggccgggg cggttctgat ccgcgacacg gaggcgatga
     7981 tgaggcaggc gggctttgcg gacatcgtcc tggaatcgaa gccggactac atcgacgcga
     8041 tgagccggtg gagcgatccg ctctaccgtg ccatcatcga gaggctgccg ccgggcgccc
     8101 gcctggcgga cttcgtaacc agcttgtcta tttccgcccg gaagccccgc ccgtagccgg
     8161 ttccgccgca gggtccagcg ccgtattcag caggtgggcg aggcggatgc cagccttctc
     8221 gagctgttcc cgggccacgg gcaccgcggc atcaatgtag ttctcccgcg tcaggtccgt
     8281 gcccggccgg gaccaggtga acgggatatc cgagccgtcc gcgaaatgat acgcccggtc
     8341 atgtgccagc ttgaagcttt cgaaggccca gtcgagcggc gcgcccgccg cccaggactg
     8401 cgcctgctcg ggcgtgattt ccgcgagcag ttgccgggcg acggcgtccg ggtcgcgccc
     8461 ggccatcagc tcgtacacca ggcattcgtc ccagacgctg tgcaggttga gcggatggtc
     8521 catcggttcg ctgtcgttca gcgtgaaatg ctcgccctcg aacgatcgga tggggatgaa
     8581 attgccgccg gcgtcgttgt accggtagcc gcagtgcagc ggctggtgca ggtcgccgac
     8641 aaagtgcaca agaaagctca cagccgcccg ccgccgcgcc cgggacagct tcggccgggc
     8701 cagctccccg tgccagcgag tgatctggct gacgatatcc tgctcgtcct cgggcagctt
     8761 caattcgtcg gcatggtcga agacgttgaa gtccacgtaa tgccagcggt tgttcttcgg
     8821 gtaccgcttg tcgtattcct tgtcgccccg gatgatgtcc ggccagctgg atacctcgta
     8881 gtcgcccagc tgcttcttgc ccaggatggc gcgcatccgc tcccgggcgc gcggggtgag
     8941 gtggcggacg gcgatgtccg ccacgatcgt gtgcccttcc ggcccccacg ccggggcggc
     9001 ggcgggccac ccggccgtca tccacgttgc ggccagcgcc agtacgatgc ggctgtttat
     9061 catgatgatt ccccggggaa gcgagccacc actatacccg aacatctcct gttttccagc
     9121 ccccgcccaa aaaggccctt cgcgcgggcc ggtttctatg cttaagtcga cggcgtgaac
     9181 tggaaggact tcttcgaccg gctgggcatg gacggcacgc gctggcagtg gcggatcatg
     9241 cgctggcagc agcggatggc ggagcgcggg gcggagcgcg ccgctcccga aaaccgcgcc
     9301 gcctcccgct acaagttctg cgaaacctgc ggcgcgatcc tgccgcgaga ggaaaaagtc
     9361 tgcccgcgct gccaggcccc cgcgccgggc tggcgatcgc aatacatcaa gcgccgtctg
     9421 gcgcttgtgc ttccaggatg ggtgcccgtc accggcgtgc tcctcgcggc caacgggctg
     9481 gtgatgctgg cggcgatgct gtttttcggc ctgggcgcgc tcttccag
//
LOCUS       DAOOTK010000117         9427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4686_length_9427_cov_6.329279, whole genome shotgun sequence.
ACCESSION   DAOOTK010000117 DAOOTK010000000
VERSION     DAOOTK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4686_length_9427_cov_6.329279"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PK634_11565"
     CDS             <1..471
                     /locus_tag="PK634_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98414.1"
                     /translation="RVRFWFVALLLPAALQAADMLPARARATVQDEGAITPTLEERLF
                     WADKTGVAAVFPASPLYRNTLPMLYFQNFPGIILVNGYSARSTDLMDGLARAGPVVGE
                     GSLKQVAACERAGVDWIVLRRADIPETRIDALRQSGRRLALDGERFAVFEALQE"
     gene            complement(481..1290)
                     /gene="dapB"
                     /locus_tag="PK634_11570"
     CDS             complement(481..1290)
                     /gene="dapB"
                     /locus_tag="PK634_11570"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOW98415.1"
                     /translation="MSNVVIVGAGGRMGKIMIRLLQAGAEPGLKLSGAVDLWDCPDRD
                     KDAGLAAGVSEAGVLITSDLAALLPACDAVVDFSAHHGTAGNAPRVAAAGKGLVIGTT
                     GLGAEEKKAVQEAARKIPVVVSPNMSLGVNLLFALVRKAAESLKGKGYDIEIIERHHR
                     KKKDSPSGTAIGLGEAAAAGYGWDLKKTAVDGRSGLVGERPAEQIGFHAVRGGDIVGD
                     HTVLFAADGECIELSHRATSREAFARGALRAAAWLAGRAPGLYSMRDVLGV"
     gene            complement(1383..2249)
                     /gene="dapA"
                     /locus_tag="PK634_11575"
     CDS             complement(1383..2249)
                     /gene="dapA"
                     /locus_tag="PK634_11575"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOW98416.1"
                     /translation="MFEGAYTAIITPFGRDGGVDYGKLRDLIERQVAGGVDGIVPVGT
                     TGESPTLDFPEHEKVIETCIEVCAGRIKVIAGTGANSTAEALELTRHAIGAGADGTLQ
                     VTPYYNKPNTQGLVRHFSAVADLGLPTVLYNIPGRTGREIPVDTVVELARHPKIVAIK
                     EAGGSVDRVSQIAGRCGLALLSGDDSLTLPMMVVGGHGVISVASNVAPKAVADMVHAA
                     LRGDWETARRLHRQYYRLFTDLFLDTNPIPVKAALAMMGQCEETYRLPLCEMSDKVKA
                     TLRDTLKQLKLL"
     gene            2443..3927
                     /locus_tag="PK634_11580"
     CDS             2443..3927
                     /locus_tag="PK634_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOW98417.1"
                     /translation="MKIIESINRDVLKKSVQRARERGVILPTFAQQKNPSFVPQKIVD
                     KLKGIGLWDINPLNLFRITWKNEPKAKGGGFGEGNWVEFPPELTGVPAKIVGLAGKFF
                     PTGAHKVGAAYGCLVPRLVSGNFDPTTQKAVWPSTGNYCRGGAYDCALLACPAVAILP
                     EGMSRERFEWLKEIGTDEIIATPGTESNVKEIYDKCWELRRERGNKIVIFNQFEEFGN
                     SIWHYNMTGGVIEEIYQKHYRTDRSRLAAYISATGSAGTIAAGDFLRTKHPFLRVVAT
                     EALQCPTLYQYGFGAHRIEGIGDKHVPWIHNVRNTDAVAAIDDEQVMQLLRLFNEPAG
                     LKFLEQRGVKREVLDRLSWLGISCICNLVAAIKTARYFDMGPNDVIFTPLTDSMDLYT
                     SRKQEMEAQHGKYDAVKAAVHYARYLEGCGVDFFRELTYFDRKALHNFKYFTWVEQQG
                     KDSDELRRLWDEDFWKELFSQAQVDEWDRLITEFNDATGVLKAL"
     gene            4058..5872
                     /gene="typA"
                     /locus_tag="PK634_11585"
     CDS             4058..5872
                     /gene="typA"
                     /locus_tag="PK634_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOW98418.1"
                     /translation="MSVAIRNIGIIAHVDHGKTTLVDCLLRQAGTFRDNEELVERVMD
                     SMDLEREKGITIKAKNASIEWGGATINIVDTPGHADFGGEVERILTMVDGVLLLVDAA
                     EGTQAQTRFVLRKAIEQGLAVVVVLNKVDRPNANVARVHDQVLELLLELHASEAQFHA
                     PFLFASARDGYAVRDLADEHKDMTPLFETILSHIPAPKADPDAPFRMVISNLDWSDYI
                     GRIAIGKVLAGSARMGDSLVCIHKDGKRERATITRVMTFRGVKTSESAVAVAGSIVGL
                     AGFEDAHIGETVCDSEEREPEPVRQIDPPTIQMQFGVNDSPLSGREGRYLTARHLKER
                     LVKETRLNVSIEIYDTDQPNFFGIRARGALQIAILAETMRREGYELLVSRPEVIYREE
                     GGVRQEPYETLWIETPDDILGDLLQHLAGRRALVTKMEHHPHGVQIEAEAPTRGLIGL
                     ESALANLTSGRAVMSRLFRGYGPVTGDIRARGAGVLVSMEGGVATSYALDTLQLRGRM
                     FIGPGDEVYEGMIVGDSARSGDLPVNPTRTKHLTNIRAAGKDDAIILEPPVELTIEKA
                     IEFIAPDEYVEVTPGHIRLRKKILNSIQRKRAGRTSGE"
     gene            5950..8328
                     /locus_tag="PK634_11590"
     CDS             5950..8328
                     /locus_tag="PK634_11590"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOW98419.1"
                     /translation="MRAVFAFTLVVCSVLAGIPAASATLYFEQNSGQFAADWLFVSRS
                     GDSGIGLSRGGMTLTLPSGRQVVVAFAGASAAARVEGRAPDGGVMNYFIGDRRMTGIP
                     RFARVRYSGVFPGVDVEYYGEAGRLEYDVIVHPGADLRSVQLRYEGAERIAIAADGAL
                     RVTMPWGELVETPPRAYQVVSGRRQAVEAAFEVAGDGVVGFRAGAHDPALDLVIDPTL
                     LYSSYIGGTDVDSAHAIAVGADEHMYVAGRTASSNTFPVSPWTPVHGGGHDVFVTKLT
                     PGGSNVVFSTFIGGSGDDMAFGLVLDSNDGPCVVGMTDSTDFPLRSARQAALGGGLDA
                     FVLQLGPSGSNLVFSTYLGGETNDGAFSVALDGEGRLYMAGVTESTNFPVRNAYQPNL
                     NVSGEGGMTQDVFLVKWRGTNIEYSTYLGGNSYDGFNEALLVPQFGRMGLAVDTGHCA
                     YIASCTVSTNFPVTANAFQKTNESFFAVAGINLYMDAFLTKFASNGLALEYSTLLGGY
                     ENEHGHDVIILSNGVACVVGETGATNFPVTLGALKPAHVTAEVAWDGFLTVVDTAVGT
                     QLVYSTYFGADDGNDQPWAVRRDADSNLWLVGYTDSTNFPVTNAVQATIAGEPDFMGD
                     YPYDVFAAKLAPAATSIVFATYLGGVGKDYGYGLALDAHGVAYLCGMTQSTNFPVLGA
                     LQPNHNLGSDVNQADGFVAMMAWPLPVPLLRFTEAAGRVDLAAQGYPSVLYRVEAVGH
                     ISDQADWQPPNPYTNWIKAEDARTTLVWTNLIIPTDTVEVVRFYRVAATNAP"
     gene            8461..>9427
                     /locus_tag="PK634_11595"
     CDS             8461..>9427
                     /locus_tag="PK634_11595"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOW98420.1"
                     /translation="MKDESLSPLDTMRHSAAHVMAAAVCRLFENVQLDIGPATDDGFY
                     YDFDLPHRLAPEDFTRIEEEMRKIIAENHPFERLEVSRAEAEQLIREKGQRYKIERLA
                     DIPEGQPITFFRSGDFIDLCRGPHLPSTGGIQAIKLLSVAGSYYRGRETNPMLQRLYG
                     TAFETQKDLDFYLKQIEEAKRRDHRVLGRDLDLFSMQEAVGPGLIHWHPRGARIRSII
                     EEFWRQEHFKNGYDLLYTPHIGRSNLWETSGHLGFYRESMYAPMKIDEVEYFIKPMNC
                     PFHIQIYKTHKRSYRDLPLRWAELGTVYRYEKAGVLAGLFRVRGFT"
BASE COUNT         1571 a         3103 c         3224 g         1529 t
ORIGIN      
        1 cgcgtgcggt tctggttcgt ggccctcctc ctgcccgccg cgctgcaggc ggcggacatg
       61 ctgcccgccc gcgcccgggc gacggttcag gacgaggggg ccatcacgcc cacgctggag
      121 gagcgccttt tctgggcgga caagaccggc gtcgccgccg tcttcccggc gagcccgctg
      181 taccggaaca cgctgccgat gctctacttc cagaacttcc cggggatcat cctggtcaac
      241 ggctactcgg cgcggtccac ggacctgatg gacggcctcg cgcgggccgg ccccgttgtg
      301 ggggagggct cgctcaagca ggtcgccgcc tgcgagcggg cgggggtaga ctggatcgtg
      361 ctccgccgcg cggatattcc ggaaacccgg atcgatgccc tgcggcaaag cggtcggcgc
      421 ctggcgctgg acggagagcg ctttgcggtg ttcgaagcgt tacaggagta accgcgccgg
      481 ctacacgccg agcacgtcgc gcatggagta caggcccggg gcccggcccg cgagccacgc
      541 cgccgcgcgc agcgcgccgc gcgcgaacgc ctcgcggctc gtcgcgcggt gcgagagctc
      601 gatgcactcc ccgtccgccg cgaacaggac cgtgtggtcg cccacgatgt ccccgccgcg
      661 cacggcgtgg aacccgatct gctccgccgg ccgttcgccg acgagtccgc tgcgcccgtc
      721 cacggcggtc tttttcagat cccaaccgta cccggccgcc gcggcctcgc cgaggccgat
      781 ggccgtgccg ctcggcgagt ccttcttctt ccggtggtgc cgctcgatga tctcgatgtc
      841 gtagcccttg cccttgagcg actccgcagc cttgcggacc agggcgaaca ggaggttcac
      901 gcccaggctc atgttgggcg agacgacgac ggggatcttc cgcgccgcct cttgaacggc
      961 tttcttctcc tcggcgccca ggcccgtggt gccgatgacc aggcccttgc ccgcggccgc
     1021 gacgcgcggg gcgttgcccg cggtgccgtg gtgcgcgctg aaatcgacga ccgcgtcgca
     1081 ggccggcagc agggccgcca ggtcggacgt gatcaggacc cccgcctcgc tgaccccggc
     1141 ggcaagaccc gcgtccttgt cgcggtcggg gcaatcccag agatccacgg cgccggaaag
     1201 cttcagcccc ggctccgcgc ccgcctgcag caggcggatc atgatcttgc ccatccggcc
     1261 gcccgcgccc acgatgacca cgttgctcat agtgcctcgg agtttgttaa ccacagagga
     1321 cacagaggtc gcggagggga tgtcctccgt gccctctgcg tcctccgcgg ttcaatcctt
     1381 cttcacagca gcttcaactg tttcaaggtg tcccgcagcg tcgccttgac cttgtccgac
     1441 atctcgcaca ggggcaggcg gtaggtctcc tcgcactggc ccatcatggc cagggcggcc
     1501 ttgaccggga tggggttcgt gtcgaggaac aggtccgtga acagccggta gtactgccgg
     1561 tgcagccggc gcgcggtctc ccagtcgccc cggagcgcgg cgtggaccat gtccgcgacg
     1621 gccttcggcg cgacgttgga ggccacgctg atcacgccgt ggccgcccac gaccatcatg
     1681 ggcagggtga gcgagtcgtc cccggagagg agggccaggc cgcagcggcc cgcgatctgg
     1741 ctgacgcggt ccacgctgcc gcccgcctcc ttgatggcga cgatcttcgg gtgcctcgcc
     1801 agttcgacga cggtatccac cgggatctcg cggccggtgc ggccggggat gttgtagagc
     1861 accgtcggca ggccgaggtc ggccacggcg gagaagtggc ggaccagtcc ctgcgtgttg
     1921 ggcttgttgt agtagggggt cacctggagc gtcccgtcgg cgcccgcgcc gatggcgtgc
     1981 cgggtcagct ccagcgcctc ggcggtggag ttggcgcccg tgcccgcgat gaccttgatg
     2041 cggccggcgc agacctcgat gcaggtctcg atgaccttct cgtgctccgg gaagtcgagc
     2101 gtcggggact cgccggtcgt gccgaccggg acgatcccgt ccacgccgcc ggcgacctgg
     2161 cgctcgatca ggtcgcgcag cttcccgtaa tccacgcctc cgtcgcgtcc aaacggcgta
     2221 atgatggctg tataggcgcc ttcaaacata acgtcctcgc tggttgtccg tccgcggcag
     2281 gcggtccctc cgccgccggg ggttggtata gtagcagggg cacgggcccc ggcaagcgcg
     2341 gaaaagccgg cctatcccgg gatggacaag cgggaggcga gaggctaatg tgtccgattg
     2401 ccgccgggat tcccttgtga agtccctcgg ccggagagaa caatgaagat catcgagtcc
     2461 atcaaccgcg acgtcctgaa gaaatccgtg cagcgcgccc gggaacgagg tgtgatcctg
     2521 ccgacgttcg cgcagcagaa gaatccgagc ttcgtgccgc agaagatcgt ggacaagctc
     2581 aagggcatcg ggctgtggga catcaacccg ctgaacctgt tccgcatcac atggaagaac
     2641 gagccgaagg cgaagggcgg cgggttcggc gagggcaact gggtcgagtt cccgcccgaa
     2701 ctgaccggcg tgccggcgaa gatcgtcggg ctggcgggca agttcttccc gacgggcgcg
     2761 cacaaggtgg gcgcggcgta cggctgcctc gtgccgcgcc tggtctccgg caacttcgac
     2821 ccgacgacgc agaaggccgt ctggccgtcc acgggcaact actgccgcgg cggcgcgtac
     2881 gattgcgcgc tgctggcctg ccccgccgtg gcgatcctgc cggagggcat gagccgcgag
     2941 cgcttcgagt ggctcaagga gatcggcacg gacgagatca tcgcgacgcc gggcaccgag
     3001 tcgaacgtga aggagatcta cgacaagtgc tgggaactgc gccgcgagcg cggaaacaag
     3061 atcgtgatct tcaaccagtt cgaggagttc ggcaactcga tctggcacta caatatgacc
     3121 ggcggcgtga tcgaggagat ctaccagaag cactaccgca ccgaccgcag ccgcctggcg
     3181 gcgtacatca gcgcgacggg ctcggcgggg acgatcgccg cgggcgattt tctgcgcacg
     3241 aagcatccct tcctgcgcgt ggtcgcgacc gaggcgctcc agtgcccgac gctctaccag
     3301 tacgggttcg gcgcgcaccg gatcgagggc atcggcgaca agcacgtgcc gtggatccac
     3361 aacgtccgca acaccgacgc cgtggccgcg atcgacgacg agcaggtcat gcaactgctg
     3421 cgcctgttca acgagccggc cggcctgaag ttcctggagc agcgcggcgt gaagcgcgag
     3481 gtgctggacc gcctatcgtg gctcggcatc tcgtgcatct gcaacctggt cgccgccatc
     3541 aagacggccc gctacttcga catgggcccg aacgacgtga tcttcacgcc gttgacggat
     3601 tccatggatc tctacacgtc ccgcaagcag gagatggagg cgcagcacgg gaagtacgat
     3661 gcggtcaagg cggcggtcca ctacgcccgt tacctggagg gttgcggcgt ggacttcttc
     3721 cgcgagctga cttacttcga ccgcaaggcg ctgcacaact tcaagtactt cacctgggtc
     3781 gagcagcagg gcaaggactc cgacgaactg cgccgcctct gggacgagga cttctggaag
     3841 gagctcttct cccaggccca ggtggatgag tgggaccgac tgatcaccga gttcaacgac
     3901 gccacgggcg tgctcaaggc cctgtaggat tcaccgcgaa tcagcgcgaa tgcacgcgaa
     3961 gtaaggaagg aaagccgtat tcgcgttaat tcgcgttaat tcgcggcccc attccggttg
     4021 cctttcccgg cgaatcgcgt agagtggccc gcctttcatg agtgtcgcca tcagaaatat
     4081 cggcatcatc gcccacgtgg accacggtaa gaccacgctg gtggactgcc tgctgcgcca
     4141 ggctgggacc ttccgcgaca acgaggagct cgtcgagcgg gtcatggact cgatggacct
     4201 cgagcgcgaa aaggggatca ccatcaaggc caagaacgcc tcgatcgagt ggggcggggc
     4261 gacgatcaac atcgtggaca cgcccggcca cgcggacttc ggcggcgagg tggagcgcat
     4321 cctgaccatg gtggacggcg tgctgctgct cgtggacgcc gccgagggca cgcaggccca
     4381 gacgcgcttc gtccttcgca aggcgatcga gcaggggctg gccgtggtcg tcgtgctcaa
     4441 caaggtggac cggccgaacg cgaacgtggc gcgcgtgcac gaccaggtgc tggagctcct
     4501 gctggaactg cacgccagcg aggcccagtt ccacgcgccg ttcctcttcg ccagcgcgcg
     4561 cgacggctac gcggtgcgcg acctggcgga cgaacacaag gacatgaccc cgctgttcga
     4621 gaccatcttg agccatattc ccgctccgaa ggccgacccg gacgccccgt tccgcatggt
     4681 gatcagcaac ctcgactgga gcgactacat cgggcgcatc gccatcggaa aggtgctggc
     4741 cggctccgcg aggatgggcg attcgctggt gtgcatccac aaggacggga aacgcgagcg
     4801 cgcgacgatc acgcgggtga tgacgttccg cggcgtgaag acctccgagt ccgccgtcgc
     4861 cgtcgcgggc tcgatcgtcg gcctggccgg cttcgaggac gcgcacatcg gggagacggt
     4921 ctgcgactcc gaggagcgcg agccggagcc ggtccggcag atcgacccgc cgaccatcca
     4981 gatgcagttc ggcgtcaacg acagcccgct ctccgggcgc gagggccgct acctgaccgc
     5041 ccggcacctg aaggagcggc tggtcaagga gacgcgcctg aatgtgtcca tcgagatcta
     5101 cgacacggac cagccgaatt tcttcggcat ccgcgcgcgc ggcgcgctgc agatcgcgat
     5161 cctcgcggag accatgcggc gcgagggcta cgaactgctc gtgtcgcgcc cggaggtgat
     5221 ctaccgcgag gagggcggcg tccggcagga gccctacgag accctgtgga tcgagacgcc
     5281 cgacgacatc ctcggcgacc tgctgcagca tctggccggg cggcgggccc tggtgacgaa
     5341 aatggagcac cacccgcacg gggtgcagat cgaggccgag gcgccgacgc gggggctgat
     5401 cgggctcgag agcgcgctgg ccaacctgac cagcgggcgc gcggtgatga gccggctgtt
     5461 ccgggggtac gggccggtaa cgggcgatat ccgcgcgcgc ggcgcggggg tgctggtgtc
     5521 catggagggc ggcgtggcga cctcgtacgc gctggacacg ctgcagttgc gcggccggat
     5581 gttcatcggg ccgggcgacg aggtgtacga ggggatgatc gtcggcgaca gcgcccgcag
     5641 cggcgacctg ccggtgaatc ccacgcggac caagcacctg acgaacatcc gcgcggcggg
     5701 caaggacgac gcgatcatcc tggagccgcc cgtcgagctg acgatcgaga aggccatcga
     5761 gttcatcgcc ccggacgaat acgtcgaagt cacgccgggg cacatccggc tgcgcaagaa
     5821 aatcctcaac tccatccagc gcaagcgcgc cggccggacc tccggcgaat aatacttgtc
     5881 tcccgccccg ttcttttcta tagggggggc gtcggcgtct tcaggaggga tgccgacaag
     5941 gaggcgagca tgagagccgt tttcgcattc acgctggtgg tgtgttcggt cctggccggc
     6001 atcccggccg catctgccac gctctatttc gagcagaaca gcgggcaatt tgccgccgac
     6061 tggctcttcg tcagccgctc cggcgattcg ggcattggcc tctcgcgcgg cgggatgacg
     6121 ttgacgctcc ccagcggccg gcaggtggtc gtggccttcg cgggggcgtc ggccgcggcg
     6181 cgggtggagg gacgggcgcc cgacggcggg gtgatgaact acttcatcgg ggaccggcgg
     6241 atgaccggaa ttccgcgctt tgcccgggta cggtactcgg gcgtgtttcc cggcgtggac
     6301 gtggagtact acggggaggc cggccgcctg gaatacgacg tgatcgtgca cccgggggcg
     6361 gacctgcgga gcgtccaact ccggtatgag ggggccgagc gtatcgcgat cgcggcggac
     6421 ggcgcgctgc gggtgacgat gccgtggggc gagttggtcg agacgccgcc ccgggcttac
     6481 caggtcgtga gcgggcggcg gcaggctgtc gaggccgcgt tcgaggtggc gggggatggc
     6541 gtggtgggat tccgggcggg cgcccacgac ccggcgctcg acctcgtgat cgatccgacg
     6601 ctgctctact cctcctacat cggcggcacg gacgtggatt ccgcgcacgc gattgcggtg
     6661 ggcgccgacg agcacatgta cgtggcgggc cggacggcct cgagcaacac gtttcccgtc
     6721 tcgccgtgga cgccggtaca cggcggcggc cacgacgtat tcgttaccaa gctgacgccc
     6781 ggcggcagca acgtggtgtt ttccaccttc atcggcggaa gcggggacga catggcgttc
     6841 ggattggttc tggattccaa cgacggcccg tgcgtggtcg gcatgaccga ctcgactgac
     6901 tttccgctgc ggtccgcccg gcaggcggcg ctgggcggcg gtctggatgc cttcgtgctg
     6961 cagctgggcc cgtccggcag caacctggtg ttttcgacgt acctgggcgg cgagaccaac
     7021 gacggcgcgt tttccgtggc gctggacggc gagggtcgcc tctacatggc cggcgtgacg
     7081 gaatccacga atttcccggt gcgcaacgcc taccagccga acctcaacgt cagcggggag
     7141 ggcggcatga cgcaggacgt cttcctggtc aagtggcggg gcacgaatat cgagtactcg
     7201 acctacctgg gcgggaacag ctatgacggg ttcaacgagg ccctgctcgt gccgcagttc
     7261 gggcgcatgg ggctcgccgt ggataccggg cactgcgcgt acatcgccag ttgcacggtt
     7321 tccacgaact tcccggtgac ggcgaacgcg ttccagaaaa ccaacgaatc cttctttgcg
     7381 gtggcaggca tcaacttgta catggatgcc ttcctgacca agttcgctag caacggcctg
     7441 gcgctcgagt actccacgct gctgggcggc tacgaaaacg agcacggcca cgatgtcatc
     7501 attttgtcca acggcgttgc gtgcgtcgtg ggcgagacgg gcgcgacgaa ttttcccgtg
     7561 acgctcggcg cgctgaagcc ggcccacgtc acggcggaag tggcctggga cggattcctg
     7621 acggtcgtcg acaccgccgt gggcacgcag ttggtctact cgacctactt cggcgcggac
     7681 gacggcaacg accagccgtg ggcggttcgt cgcgacgcgg acagcaacct gtggctcgtg
     7741 ggctacacgg attccacgaa tttcccggtc acgaacgccg tgcaggcaac gatcgccggc
     7801 gagcccgatt tcatgggcga ttatccctat gatgtgtttg cggccaagct ggcgccggcc
     7861 gcgacgtcca tcgtgttcgc gacgtacctg ggcggcgttg gaaaggacta cgggtacggg
     7921 ctggcgctgg acgctcacgg cgtggcctat ctctgcggca tgacgcagtc caccaacttc
     7981 cccgtcctgg gcgcgctcca gccgaaccac aatctgggct ccgatgtcaa ccaggccgac
     8041 ggtttcgtcg ccatgatggc ctggccgctg ccggtgccgc tgctccggtt caccgaggcc
     8101 gcggggcggg tggacctggc cgcgcaggga tatcccagcg tgctctaccg ggtcgaagcg
     8161 gtcgggcata tatccgatca ggccgactgg cagccgccca atccctacac gaactggatc
     8221 aaggccgagg acgcgaggac gacgctggtc tggaccaatc tcatcattcc caccgatacc
     8281 gtggaagtgg tccgcttcta ccgcgtagcc gccaccaatg cgccataatc cgccgcgatg
     8341 gtttctcgtc gggaagtggc ttccgctgcc tttccatccc cgcatggatt gacagccccg
     8401 ggtggcatcg ggtaagttgc gccgtgtaat gcggtaaaga aaggaatgta tcgagcctga
     8461 atgaaggatg agagtctgtc ccccctggac accatgcgcc acagcgcggc gcatgtcatg
     8521 gcggcggcgg tttgccgcct gtttgaaaac gtccagctcg acatcggtcc cgccaccgac
     8581 gacggtttct actacgactt cgatctcccg caccgcctcg cgcccgagga ttttacccgg
     8641 atcgaggagg agatgcggaa gatcatcgcg gaaaaccacc cctttgagcg gctcgaggtc
     8701 tcgcgcgccg aggcggaaca gctgatccgg gagaagggcc agcgctacaa gatcgagcgc
     8761 cttgcggaca tccccgaggg ccagccgatc acgttcttcc ggagcggcga cttcatcgat
     8821 ctttgccgcg gcccgcattt gccctccacc ggcgggatcc aggccatcaa gctgctctcc
     8881 gtcgcgggct cgtactaccg cggccgcgag accaacccga tgctccagcg cctgtacggc
     8941 acggcgttcg agacgcagaa ggacctggat ttctacctga agcagatcga ggaggccaaa
     9001 cgccgcgacc atcgcgtcct cggccgcgac ctggacctgt tcagcatgca ggaggcggtc
     9061 gggcctggcc tgatccactg gcacccgcgc ggggcgcgca tccggtcgat catcgaggag
     9121 ttctggcggc aggagcactt caaaaacggc tacgacctgc tctacacgcc gcacatcggg
     9181 cgcagcaacc tgtgggagac ctccgggcac ctcgggttct accgcgagag catgtacgcg
     9241 ccgatgaaga tcgacgaggt cgagtacttc atcaagccga tgaactgccc gttccacatc
     9301 cagatctaca agacgcacaa gcgctcgtac cgcgacctgc cgctgcgctg ggcggaactg
     9361 ggcacggtct accgctacga gaaggccggc gtcctcgccg ggctcttccg cgtccgcggc
     9421 ttcaccc
//
LOCUS       DAOOTK010000118         9266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4778_length_9266_cov_4.085658, whole genome shotgun sequence.
ACCESSION   DAOOTK010000118 DAOOTK010000000
VERSION     DAOOTK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4778_length_9266_cov_4.085658"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK634_11600"
     CDS             <1..954
                     /locus_tag="PK634_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98421.1"
                     /translation="WVGYGEIDQTVSSNFWTLVYETNSTVQRDITTTIENGTNTVKDE
                     TVTTISTSLVGTNIITSTNYNSTETMIGLASSYIFPNGSIGTLDVRERSFDGDLAGES
                     VLTYSMEYLWRPVLPLDMSIRYEHGLVPSARAIVEYDGGGIGAIWRAKDEWNATGTAA
                     YWSLDDDNSLLRLMLENYWRISERYDIWLGLHDSLITADRASDLYWTPYWEQRHYLII
                     RLRRSYPNYFGMVRVNVGAQQAEGRPEEWAVYNERAARAEGQGWYPGNDPDQDWERLI
                     GFSASLRRSWANGWEIEGEATVNSLREQTERSLIGSVVYRF"
     gene            995..1693
                     /locus_tag="PK634_11605"
     CDS             995..1693
                     /locus_tag="PK634_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98422.1"
                     /translation="MTVGLVLLLGVLTPARAAEGPQRILILGDSMMRVPSHSIELEFS
                     RHEGVTTRLFTSLGSGLARLDVVDWLERIRALAAEFQPDTSIIWIGTNDEQPMRTPAG
                     IIRPEQPGWSEEYARRVGEAMDLMGRGGAAKVYWLELPDMPKPEIQKSADRINGLVDQ
                     EAAKRPFVIHCKTRPFLSRTPGTFSRYVLRPNGMPLSVRDTDGVHLDRNGADLLARKL
                     AETFWPAAPAATEK"
     gene            1690..5448
                     /locus_tag="PK634_11610"
     CDS             1690..5448
                     /locus_tag="PK634_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98423.1"
                     /translation="MTFGLSYGRGRWPAAARAGWAILLLTGLAAAAQEPAPPAAAAGV
                     VREALPSTPVAPAGVAVPLVAGEVAPAAAPVGETEDVARVTPAMAKMYEAMVNIQDGL
                     YDEAIPKLEWVIETDPSLYGAWEALCAAYWQSGRHEEARRTGERFLAIAPQNPRAHNF
                     LAQVATSDGNLDRAEELYRKSLALNPNQYDARFSYARVLFWNGKRELALEELKRLYQE
                     DTARLDVEIQLARAYYANEEYELALEHWNNINEMVPDYPEYMIARAQVLVLIGALKEA
                     QLEAERVLEVEPDNEAALNLLTDISMRARRHDEVIQNLRRVRDLAKGDLAKAKIARRL
                     AYYLKSQYDRDPKNFTMKQCVQAAQDAYRWDPGDVDGVLFYGEMLVLDKEYGRAEERF
                     RDVLQNFNPHNQRAKFGLYETFLGRMKLDEAEAQLEAIMVDFDPNDPYRHYFWAQLYF
                     AQGRYFDAQESLERLEQEGAEGSVFTLLYHGLSPSEWAAVPSVRQFRDHMMTLKREGY
                     KFIQPEQFADYFAKQKPPDLVERRSLLTRMVQAVRRSFSAEQPEPVVTVRDYSPERIV
                     CVTFDDGMRSSFRWATPVAEELRIPLTMFVPVGNILSNDTRITSWPELRAYERTGWWT
                     LGSHLMDASTPTFTNRPTTNRVVEVVLPATTNALPATNLLAELDASAMTNFILEAGPP
                     MITNRMELTDANLRRVHPLPNLLWVREKERQETLREYYRRIRYEFLDSRNWLRRELEL
                     PEGILAVAYPMSDVGQQTYCNIDLFNVPEAVLNEAELHYRMGFRQSRFGYAMKRDNPM
                     LYQRMEPDRHDSGQDLLRDALRNHPVFLARRTRAEMAAQQGEMHTALDMIELLKRDNY
                     PEEDLAELSAYVQRRLARLVAIPEAVKDETDEERKRLVDIRHPYLGVEGESVKANAMI
                     DEWRVALKGGFNVNPRWLIEGRVGYGSIAQEVTSNEWTEVFVTNVSTRQTISATTENG
                     TNSLEDETIVSFVQTLQGTNIVHTTNYSASETMIGLASAYIFPNGSVAHFDIRQRTFD
                     DDLAGESVIAYSLEYMWRPALAIDMAIRYEHDMTPSAREIIEYDGAALGAVWRVRDWW
                     NAAGQGVYRMMDDDNSLLRVMAENFWRISQRYDIWVGLQNSLVTTDMDSDLYWTPYWD
                     QRHVAVIRVRRVFPNYYGTVRVNVGLQKAKGRPEEIELYRSRAAVGEIQGWYPGEGPD
                     EDWNKVVGVGASVIRRWANGWEIKGETSINAIGETTERHLTGSLIYRF"
     gene            complement(5467..6954)
                     /locus_tag="PK634_11615"
     CDS             complement(5467..6954)
                     /locus_tag="PK634_11615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98424.1"
                     /translation="MESTPAATSRQPADSAPRLPPRKGMIGHCSACEMSIRVIARPLP
                     AVCPFCLRRLQTEGYEPEAPTPEPQAVLDGVAVHRYRRFPRWLARLLAAFSIIVLATV
                     VMMKIRNLEYFWYQPWVNTYSICVGVFILSRFLLAAFYTSPVDVGYEPTVCVAIACKN
                     EEGSIETTIGRIYREGYPHAKLEVVAVNDGSTDRTLQDMIRAQERHPSLVIVDFIRNK
                     GKRHGMAVGALLATAEVLVYVDSDSFLLPGAIRKVVQGLVDPTVAAVSGHTDVENVDV
                     NMLTKMQDVRYFVSYRVMKAAESLFGCVSCCPGCFSAYRRACVLHVLDRWLHQKFLGV
                     YSTYGDDRSLTNYLLRDYKILYDDEALATTIVPERWKKYIVQQCRWKRSWVRELLFAG
                     RFVWRKHPVAAISWYALTLLPMIAPLVMFRALVAWPLMTGQLPGFYITGVLVVTLLWG
                     LYYFERTGRPHWWTAFLFMMSYVVFFSWQGYYALATMRSNKWGTR"
     gene            complement(7037..>8639)
                     /locus_tag="PK634_11620"
     CDS             complement(7037..>8639)
                     /locus_tag="PK634_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98425.1"
                     /translation="ARWRQLVKFAAQYYQRSLDISPGQLDLVIRMSRLMNGGDEGPGS
                     ALALLNQYASIFPNQPEIVLGKAEWLARHGRDQEAGELIHQVLEQHPNNLQARSLLHA
                     LLKDPRERYDNLRSIVEVGSLPGMEMPFVAAIRDHQLLTLPESWMLMNALESIAGRAS
                     AENRDVFTALLPRRRAAREDFRSDRLSPDWISSVEVRSHDPGRLILAADPFETEAYVQ
                     LDRSEGLHSGYIETVIDQPRGHFWIYARRSVHGMVRFGFDPEGQIYLQVWRGDQIIAN
                     EVQPWTRPDGPLRLRLEIRGDGAQGYVEGAPVFRTPVSLPLDLSLGWWGAGAWAAQFG
                     IAQVTLREIEGGPLPVRLAGFHHREQPLSDDACVEMLKPLVQDLSAVAPFWYRHEMDG
                     TIRPDGDQDFRKVRIFARYHRLRLLPVVRLCDWRSLDPAELVRRATEEEVDGFTLMML
                     QLPPPEWIERAEQAMLGAPLTFLAVFMDEDSRAATLREIAPDAGLLPGARKIHRLPIL
                     LPEELTVDEPDGLWRRPPGNAVVLF"
     assembly_gap    8640..8649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8650..>9266)
                     /locus_tag="PK634_11625"
     CDS             complement(<8650..>9266)
                     /locus_tag="PK634_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98426.1"
                     /translation="LEDNAGIGRLEPESLAAQLAEIGADGVLLDFTRADAGLSPPILT
                     AWLQKLHALLAEQRRLLIVRFPRYVVHDAALKALMSVLPNLLVGTSRDPALAVPLSRL
                     GDEADRVILVEEAEPAPEETPVGVFYELAPGGRESRRSETESPSEIWRRRGQAAFLSG
                     SFQHAIEIWTEWHVAEPYNEEPLTLIGDVYLRRGDIPTALQYYQR"
BASE COUNT         1587 a         3117 c         3206 g         1346 t
ORIGIN      
        1 tgggtcggct acggggagat cgaccagacg gtctcctcca acttctggac cctggtctac
       61 gagacgaatt ccacggtgca gcgcgacatc acgaccacga tcgagaacgg gaccaacacc
      121 gtgaaggacg aaaccgtcac gaccatctcg acctcgctgg tcggcaccaa catcatcacc
      181 tcgacgaact acaactccac cgagacgatg atcgggctgg cctcgtccta catcttcccc
      241 aacggctcga tcggcaccct ggacgtccgg gagcgcagct tcgacggcga cctcgccggc
      301 gagtccgtcc tgacctactc gatggaatac ctgtggcgcc cggtcctccc gctggacatg
      361 tccatccgct acgagcacgg cctggtgccg tcggcccgcg cgatcgtcga gtacgacggg
      421 ggcggcatcg gcgcgatctg gcgggccaag gacgagtgga acgccacggg gaccgcggcc
      481 tactggtcgc tggacgacga caactccctg ctgcgcctga tgctggaaaa ctactggcgc
      541 atctccgagc gctacgacat ctggctgggg ctgcacgact ccctgatcac cgccgaccgg
      601 gccagcgacc tgtactggac gccgtactgg gagcagcgcc actacctgat catccggctg
      661 cggcgcagct acccgaatta cttcggcatg gtccgcgtga acgtcggggc ccagcaggcc
      721 gagggccgcc ccgaggaatg ggccgtctac aacgagcgcg cggcgcgggc ggaggggcag
      781 gggtggtacc cgggcaacga ccccgaccag gactgggagc ggctgatcgg gttcagcgcg
      841 agcctccgcc gctcctgggc caacggctgg gagatcgagg gcgaggcgac cgtcaactcg
      901 ctgcgggagc agaccgagcg cagcctgatc ggcagcgtgg tgtaccggtt ctagggacga
      961 ggcgagcgga ggaacgaaca tgaagcaggc ggcgatgaca gtcgggctgg tgctgctgct
     1021 gggcgtcctg acgccggcgc gggccgcgga ggggccgcag cgcatcctga tcctcgggga
     1081 ttcgatgatg cgcgtgccct cgcactcgat cgagctcgaa ttctcccggc acgagggcgt
     1141 gaccacgcgc ctgttcacct ccctcgggtc cgggctggcc cggctggatg tcgtggactg
     1201 gctggagcgc atccgcgccc tggccgcgga attccagccg gacacctcga tcatctggat
     1261 cggcacgaac gatgagcagc ccatgcgcac gccggccggc atcatccgtc ccgagcagcc
     1321 gggctggtcg gaggaatacg cgcggcgggt cggcgaggcc atggacctca tggggcgcgg
     1381 gggcgcggcg aaggtgtact ggctcgaact gccggacatg cccaaaccgg agatccagaa
     1441 aagcgccgac cgcatcaacg gcctcgtgga ccaggaagcg gcgaaacggc ccttcgtcat
     1501 tcactgtaaa acccgcccgt tcttaagccg gacgcccgga accttctcgc gttacgtgct
     1561 gcgcccgaac gggatgcctc tttcggtgcg cgacaccgac ggcgtgcatc tggaccggaa
     1621 cggggcggat ctgctggccc ggaaactggc cgagacgttc tggcccgccg cgccggccgc
     1681 aactgaaaaa tgacattcgg actttcatac ggccgggggc ggtggccggc cgcggcgcgg
     1741 gcgggatggg cgatcctgct gctgaccggc ctggccgccg cggcccagga accggcgccg
     1801 ccggcggcgg ccgcgggcgt ggttcgcgag gccctgccgt ccacgccggt tgctccggcg
     1861 ggcgtggccg tgccgttggt tgcgggcgag gtggcgcccg cggccgcccc ggtcggggaa
     1921 accgaggatg tcgcccgcgt gacgcccgcc atggcgaaga tgtacgaggc catggtgaat
     1981 atccaggacg ggctctatga cgaggccatc cccaagctcg agtgggtgat cgagacggac
     2041 cccagcctgt acggcgcgtg ggaggccctg tgcgcggcct actggcagtc gggccgccac
     2101 gaggaggcgc gccggacggg cgagcggttc ctggcgatcg cgccgcagaa tccgagggcg
     2161 cacaacttcc tcgcgcaggt ggccacgtcc gacggcaacc tcgaccgggc cgaggagctc
     2221 taccggaaga gcctggcgct gaatcccaac cagtacgacg cccggttctc gtacgcccgc
     2281 gtcctgttct ggaacgggaa gcgggagctc gcgctggagg agttgaagcg gctgtaccag
     2341 gaggataccg cgcgcctcga cgtcgagatc cagctggccc gggcctacta cgccaacgag
     2401 gaatacgagc tggcgctgga gcactggaac aacatcaacg agatggtgcc cgattacccg
     2461 gaatacatga tcgcccgggc gcaggtgctg gtcctgatcg gcgcgctgaa ggaggcgcag
     2521 ctcgaggccg aacgcgtcct ggaggtcgag cccgacaacg aggccgcgct gaacctcctg
     2581 acggacatct ccatgcgcgc gcgccggcac gacgaggtga tccagaacct ccgccgcgtc
     2641 cgggacctcg ccaagggcga cctggccaag gccaagatcg cccggcggct ggcctactac
     2701 ctgaagagcc agtacgatcg cgatcccaag aacttcacga tgaagcagtg cgtgcaggcc
     2761 gcgcaggacg cctaccgctg ggaccccggc gacgtggacg gcgtcctgtt ctatggcgag
     2821 atgctggtgc tggacaagga gtacggccgg gccgaggagc gtttccggga cgtgctccag
     2881 aacttcaacc cgcacaacca gcgcgcgaag ttcggcctct acgagacgtt cctcggccgc
     2941 atgaagctcg acgaggcgga ggcgcagctg gaagcgatca tggtcgattt cgatcccaac
     3001 gatccgtacc ggcattactt ctgggcgcag ctctactttg cccagggccg gtacttcgac
     3061 gcgcaggagt cgctggagcg gctggagcag gagggcgcgg aggggtccgt cttcacgctg
     3121 ctgtaccacg ggctctcgcc cagcgaatgg gcggccgtcc cgtcggtccg gcagttccgc
     3181 gaccacatga tgaccctgaa acgcgagggc tacaagttca tccagccgga gcagttcgcc
     3241 gactacttcg cgaagcagaa gccgccagac ctcgtcgagc gacgctcgct gctcacccgc
     3301 atggtgcagg cggtccggcg gtccttctcc gcggagcagc cggagccggt ggtcacggtg
     3361 cgcgattaca gccccgagcg catcgtgtgc gtgacctttg acgacggcat gcggtccagc
     3421 ttccgctggg cgacgcccgt cgccgaggaa ctgcgcattc ccctgaccat gttcgtgccg
     3481 gtcgggaaca tcttgagcaa cgacacccgc atcaccagct ggccggagct gcgggcctac
     3541 gagcggaccg gctggtggac gctcggcagc cacctgatgg acgctagcac gccgaccttc
     3601 acgaaccgcc cgacaacgaa ccgcgtggtg gaggtggtgc tccccgcgac caccaacgcg
     3661 ctcccggcga ccaacctcct ggcggagctg gacgcgtcgg ccatgaccaa tttcatcctc
     3721 gaggccgggc cacccatgat caccaaccgg atggagctga cggacgccaa cctccggcgg
     3781 gtgcacccgc tgccgaacct gctctgggtc cgggaaaagg agcggcagga aaccctgcgc
     3841 gagtattacc ggcgcatccg ctacgagttc ctggacagcc gcaactggct ccgccgcgag
     3901 ctggagctgc cggagggcat cctcgccgtg gcctatccca tgagcgatgt ggggcagcag
     3961 acctactgca acatcgatct cttcaacgtg cccgaggccg tcctgaacga ggccgagctc
     4021 cactaccgca tgggcttccg ccagagccgg ttcggctacg ccatgaagcg ggacaacccc
     4081 atgctgtacc agcggatgga gccggaccgc cacgactcgg ggcaggacct gctgcgcgac
     4141 gcgctgcgca accaccccgt gttcctcgcc cgccgcaccc gcgccgagat ggccgcccag
     4201 cagggcgaga tgcacacggc cctcgacatg atcgagctgc tcaagcggga caactatccc
     4261 gaggaggacc tggccgagct gtccgcctac gtccagcgcc ggctcgcgcg cctggtggcc
     4321 atccccgagg ccgtcaagga cgagaccgac gaggagcgca agcggctggt ggacatccgg
     4381 cacccctacc tgggcgtcga gggcgaatcc gtcaaggcca acgcgatgat cgacgagtgg
     4441 cgcgtcgcgc tcaagggcgg gttcaacgtc aacccgcggt ggctgatcga ggggcgcgtc
     4501 ggctacggca gcatcgccca ggaggtcacc tccaacgagt ggacggaggt cttcgtcacg
     4561 aacgtcagca cgcgccagac gatctccgcc accaccgaga acgggacgaa ctcgctggag
     4621 gacgagacga tcgtatcctt cgtgcagacg ctccagggca cgaacatcgt ccacacgacc
     4681 aactacagcg ccagcgagac gatgatcggg ctcgcctcgg cctacatctt cccgaacgga
     4741 tccgtggcgc acttcgacat ccggcagcgg acctttgacg acgacctggc cggcgagtcc
     4801 gtgatcgcct actcgctgga atacatgtgg cggcccgcgc tggcgatcga catggccatc
     4861 cggtacgagc acgacatgac gccgtcggcg cgggagatca tcgagtacga cggcgccgcg
     4921 ctcggggccg tctggcgcgt gcgagactgg tggaacgccg ccggccaggg cgtgtaccgc
     4981 atgatggacg acgacaactc cctgctgcgc gtgatggccg agaatttctg gcgcatctcg
     5041 cagcgctacg acatctgggt gggcctgcag aattcgctgg tcacgaccga catggacagc
     5101 gacctctact ggacccccta ctgggaccag cgccacgtgg ccgtcatccg cgtgcggcgc
     5161 gtcttcccca actactacgg cacggtgcgc gtgaacgtcg gcctgcagaa ggccaagggg
     5221 cgccccgagg aaatcgagct ctaccggtcc cgcgcggcgg tcggggagat ccagggctgg
     5281 tatcccgggg aggggccgga cgaggactgg aacaaggtcg tcggggtggg ggccagcgtg
     5341 atccgccgct gggccaacgg ctgggaaatc aagggcgaga cgtccatcaa cgccatcggc
     5401 gagacgaccg agcgccacct gacgggcagc ctgatctacc ggttctgacc ggggcggccg
     5461 ccgggatcag cgcgtgcccc acttgttgga ccgcatcgtg gccagcgcgt agtagccctg
     5521 ccagctgaag aacaccacgt agctcatcat aaagaggaag gccgtccacc agtgcggccg
     5581 gccggtccgc tcgaagtagt agaggcccca gagcagggtc accacgagca cgcccgtgat
     5641 gtagaagccc gggagttgcc cggtcatcag gggccaggcc accagcgcgc ggaacatcac
     5701 gagcggcgcg atcatgggca gcagcgtcag cgcgtaccag gagatcgcgg cgaccgggtg
     5761 cttgcgccag acgaatcggc cggcgaacag cagctcccgc acccaggacc gcttccagcg
     5821 gcactgctgg acgatgtatt tcttccagcg ctcgggcacg atcgtcgtcg ccagcgcctc
     5881 gtcgtcgtac aggatcttgt agtcccgcag gaggtagttc gtcaggctgc ggtcgtcgcc
     5941 gtacgtcgaa tagacgccca ggaacttctg gtgcagccac cggtcgagga cgtgcagcac
     6001 gcaggcgcgc cggtaggccg agaagcagcc cgggcagcag ctgacgcagc cgaacaggct
     6061 ctcggcggcc ttcatgacgc ggtacgagac gaagtagcgc acgtcctgca tcttcgtgag
     6121 catgttgacg tccacgttct cgacgtccgt gtgccccgag acggccgcca cggtcggatc
     6181 caccagcccc tggacgacct tgcggatcgc cccgggcagc aggaagctgt cggagtccac
     6241 gtagaccagg acctctgccg tggccaggag cgcgccgacg gccatcccgt gccgcttgcc
     6301 cttgttgcgg atgaaatcca cgatgacgag gctgggatgc cgctcctgcg cccggatcat
     6361 gtcctgcagc gtccggtccg tcgagccgtc gttgacggcg accacctcca gcttcgcgtg
     6421 gggatagccc tcgcggtaga tccggccgat ggtggtctcg atcgacccct cctcgttctt
     6481 gcaggcgatc gccacgcaga ccgtcggctc gtagccgaca tccaccggcg aggtgtaaaa
     6541 cgccgcgagg aggaagcggg agagaatgaa caccccgacg cagatgctgt acgtgttgac
     6601 ccacggctga taccagaaat actccaggtt ccggatcttc atcatgacca cggtggccag
     6661 cacgatgatg gagaaggccg ccagcaggcg cgccagccag cgggggaacc ggcggtagcg
     6721 gtggacggcc accccgtcga gcacggcctg cggctccggg gtgggggcct ccggctcgta
     6781 gccctccgtc tgcagccggc gcaggcagaa agggcagacg gccggcagcg ggcgggcgat
     6841 gacgcggatg gacatctcgc aggcggagca gtgcccgatc atccccttgc ggggcggaag
     6901 gcggggcgcc gaatccgcgg gctgtcggct ggtggccgct ggcgtcgatt ccatgcgctg
     6961 ttccatgcgg gtccggccga ccgccggccg gaacatccga tccggattat ggcgcaggag
     7021 ccgccgcctg tcatcatcag aaaagaacca cggcgttgcc cggcggccgg cgccacaagc
     7081 cgtcgggctc gtccacggtc agctcttccg ggaggaggat gggcagccgg tgaatcttcc
     7141 gcgcgcccgg aagcagcccc gcgtcggggg cgatctcgcg cagggtggcg gcgcggctgt
     7201 cctcgtccat gaagaccgcc aggaaggtca gcggggcgcc cagcatggcc tgctcggcgc
     7261 gctcgatcca ctccggcggc ggcagctgca gcatcatcag ggtgaagccg tccacttcct
     7321 cttccgtggc ccgtcgcacc aactccgccg ggtcgaggct gcgccagtcg cacaaccgga
     7381 cgaccggcag cagccggagc cggtggtagc gcgcgaagat ccggaccttg cggaagtcct
     7441 ggtcgccgtc cggccggatc gtgccgtcca tctcgtgccg gtaccagaag ggcgccaccg
     7501 ccgagaggtc ctgcacgagg ggcttgagca tctccacgca ggcgtcgtcc gagagcggct
     7561 gttcgcggtg gtggaacccc gcgaggcgga cgggcagggg accgccttcg atctcccgca
     7621 gcgtgacttg cgcgatgccg aactgcgccg cccaggcgcc ggcgccccac cagcccaggc
     7681 tcagatccag cggcagcgaa acgggcgtcc ggaaaacagg cgctccctct acgtatccct
     7741 gggccccgtc gccgcggatt tccaggcgca ggcgcagcgg gccgtcgggc cgggtccacg
     7801 gctggacttc gttggcgatg atctggtcgc cgcgccagac ctgcaggtag atctgcccct
     7861 ccgggtcgaa tccgaagcgc accatgccgt ggacgctccg ccgcgcgtaa atccagaaat
     7921 gcccgcgcgg ctggtcgatg acggtctcga tgtacccgct gtgcaggccc tcggaccggt
     7981 cgagttgcac gtaggcctcg gtctcgaacg ggtcggccgc gagaatcagc cggcccggat
     8041 cgtggctccg gacctcgacc gagctgatcc agtcgggcga aagccggtcg gagcggaaat
     8101 cctcgcgggc ggcccggcgg cgcgggagca gggcggtgaa gacgtcgcgg ttttcggcgg
     8161 aggcgcgccc ggcgatggac tcgagggcgt tcatcagcat ccaggactcc ggcagggtca
     8221 gcagctggtg gtcgcggatg gccgcgacga agggcatttc catgccgggc agcgaaccca
     8281 cctcgacgat gctccgcagg ttgtcgtagc gctcgcgggg atccttgagc agggcgtgca
     8341 gcaggctgcg cgcctggagg ttgttgggat gctgctccag cacctggtgg atcaactctc
     8401 ccgcttcctg gtcgcggccg tggcgcgcca gccactcggc cttgccgagc acgatctccg
     8461 gctggttggg aaagatgctg gcgtactggt tgagcagggc gagggcgctg cccggcccct
     8521 cgtcgccgcc gttcatcagg cgcgacatgc ggatcacgag gtccagctgg cccgggctga
     8581 tatcgaggct gcgctggtaa tactgggccg cgaacttcac cagctggcgc cagcgtgcgn
     8641 nnnnnnnnng cgctggtaat actggagcgc ggtcgggatg tcgccgcggc ggaggtagac
     8701 gtcgccgatc agggtcaacg gttcctcgtt gtagggctcc gccacgtgcc actcggtcca
     8761 tatctcgatg gcgtgctgga agctgccgga gaggaaagcc gcctggccgc gccgccgcca
     8821 gatctcggac gggctctcgg tctcgctccg ccggctttcc cttccgcccg gagccagttc
     8881 gtagaacacg ccgacggggg tttcctcggg cgccggctcg gcctcctcga ccaggatcac
     8941 gcggtccgcc tcgtccccga ggcgggacag cgggacggcg agcgccgggt cccgcgaggt
     9001 gccgaccagc aggttgggca ggacggacat cagggccttc agggccgcgt catggaccac
     9061 gtagcgcggg aagcggacga tcagcagccg ccgctgctcg gccaggagcg cgtgcagttt
     9121 ctggagccag gcggtcagga tgggcggcga cagcccggcg tccgcgcggg tgaaatcgag
     9181 cagcacgccg tccgcgccga tttccgccag ctgggcggcc agggattccg gctcgaggcg
     9241 cccgatgccc gcgttatcct cgagat
//
LOCUS       DAOOTK010000119         9159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4832_length_9159_cov_5.195409, whole genome shotgun sequence.
ACCESSION   DAOOTK010000119 DAOOTK010000000
VERSION     DAOOTK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4832_length_9159_cov_5.195409"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PK634_11630"
     CDS             <1..261
                     /locus_tag="PK634_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98427.1"
                     /translation="FLRQLSLSKEFIERREECQVEKLYVSGGMSLSRFWISDMKQASG
                     LDVERWNPFDGLHASPDALPAALAGQETRFTAAIGACVGAWE"
     gene            264..953
                     /locus_tag="PK634_11635"
     CDS             264..953
                     /locus_tag="PK634_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98428.1"
                     /translation="MTARINLLKTTEYRRQGLVSTAFIIRVSIMTVVLLALVFGALAF
                     LQRRNARQTLDSARELWRVREPLYRKTLAMKQDLATKRKLDQELRGWSASRIAWNEPL
                     AEFQEITLPTLQFRRLNVRGEMDVRVPKVKTPKAGSAEAKPAEAAAPALAAGTPARRF
                     FLLIEGRAEGDQAEDAVLQFDAALRRAPGLRALMESVRLQRLESEESRAGGAAGQVFA
                     IEAATARREMP"
     gene            956..1606
                     /locus_tag="PK634_11640"
     CDS             956..1606
                     /locus_tag="PK634_11640"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HOW98429.1"
                     /translation="MATLKKEQMQLIFLAGIWIVGGLIALYVWVVGPALRNRSRSTGE
                     LDTLNENIQKAKLAVQGESKLRKEYDQAIAEIRQAMEQYIVPPESALSWVTEKVYATA
                     RTVGVEVRSVTPVRMPDTAWDGLVKSGRFVRPYAVQLTLECGYEKLRALVRAFETGNP
                     YLCVTGLNVLGQPDQSPARHQVMLQIEWPMWGRPLGIELPGEEEEPPAKLEAENRG"
     gene            1671..2561
                     /locus_tag="PK634_11645"
     CDS             1671..2561
                     /locus_tag="PK634_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98430.1"
                     /translation="MRTKHAAWRRNFLFAMAVLAGPGCETDDNVDSGGIAGYFENNPY
                     TSLERLEGYEYLRITPASAEVTFVGQTVLFQVDGGTPPYEWSVGLPGVGSVGSVRENE
                     AVYTAGAIAANNVLVFDVAGRSGVAAINGGIETETLAVNADPDTLAVDGDRTAVNVSG
                     GVPPYRWTVLDGDLGSIDRSSGESVVYTRLRSGDNSVRVTDSEGSTGAVVIQQPASGG
                     ALTASASPSSLPNNGDRATLTASGGTPPYRWYLQDAALGSLDTDGGSSVTYTRNRPGD
                     NSVRVTDANDDTVTVLIQQP"
     gene            2572..8613
                     /locus_tag="PK634_11650"
     CDS             2572..8613
                     /locus_tag="PK634_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98431.1"
                     /translation="MKARRIMLLGAAALGLGFHAGPARGQVLFPTSGNVTAGDTFPFL
                     VTFLNEDTNDPPTKIYMFSEDPDRLSVPSQPVTQATLYNPATSNFYYRSWLDGAEGLI
                     AAGLKPVDGLFSPIRVHFVGVQYGIVWSDYADIVVDPPTFVYQPAAPALRVGETVPLA
                     IRRTIDANATLNFQVQSAWPAVVGLGTNAVGLQTNASLNLQFGVFEISKTIYVSGLAA
                     AQDAGSTNVTVTARVGSYATNVVCLVSTNAGLILAPASPSVGAGDAVAMTVTRPSATN
                     VSALTVYLTSDDPTVVGVPASVVIPQGAQSVGFSATGLRPGSSFVRATAIGVEPGPGS
                     AREVVVGNPLLAFDPDPATVPQAELRVITVRRPAMEAGGSLTVPLVNNAPTLFELSAT
                     NVTIPAGFADTTFTILGLSEGSGSLSARSGGYLTNLAVNVTAATNEDDPDGDGIPTDW
                     ELVLGSDPYDAFSLDPGLTLNDGEWDSDGEGLSNYEEIINYATDPLRGDTDDDGVDDR
                     TEVRDDITSPLHPMSSVLYRERSLDLGAVPAGGIRLPDPSRFYVRTNGWTVECWLRPT
                     TDGSGRVFALEGAGAGQSFRVSLEDFRPKAEILSGASVIASAGGVGPAGSIQQLPTNE
                     WSHVAWAWGPQDNSLKIYVNGVLLIAQETLVSPDFSAGTGVLAQAFTDGYLDDARFWN
                     YDRSWEDLDYWNNRFYPSPEGYVRPPTYGQSLRMYYRFDDGGSNTVDFAFLNQPAYFL
                     PDTATMVVTNPAVSLLGRDDEDGDLLPEWWVTMHNLDQYPEFDYGPKFEYIEVPGNWA
                     RISYFRTFRAYTSVGNETGWAQPPDNLYHEPKDQGLGHDGRHSAFMKYVYLYSAPRSA
                     TLEIFTPGMESTLVYVNGELLTPTNDTANTAQSLDVAGRLKIGRNMVYVRCISSFDTW
                     LDEARTQPVKVTDPPVQYERALGKFDARLTVDGIEHIVRGDTSRNDPRAAWFVQTWST
                     FWQMINGDEHAARPDKEIRFLPGNPDYGLPFDADTDDFNAWYEFLCGANPRDDDSNNN
                     GIPDGLEDFDGDGLVNRGEQERGTHPLLPDTDDDRRVDGAEVGAGEDPASAQSPAISR
                     ALSFSTNGSDYVEMPMQRRFALDSWTLESWVRLAPGEADGGVLVQRVVGPSGVNYELG
                     LGDGVTAPANVPYVRYVSVDGFTVQATGSVALAGGTNWTHVAGTYDGFLRELQLYTGG
                     VYVATAPQALQSPAIYAGGPVQQRIGPGLQGAVDDVRIWSVARTASEIASSFDSALQG
                     SEPNLVAYYRADDSTSYRTNPLVGTSANNGTNASVNVMPWTWGQVQDYVRGYSGDWWD
                     KWNHAATLRGRAGHTADGDGALIIPPSLRVTLLPPPAVDDGAQWAIQGLGSWRDSGVT
                     LYEGLDEGPQVILFKSIDGWTAPSNQTVTLSNGVLTSVTATYIQNGSLRVEFSFVDEA
                     THGAIWPTDGAQWRIGSGVWHEENDTVDNLTPGEYIVQFKSIDGWVPPAPSPVTILSG
                     ETTLLLGQYVPATGSLRVFIEPEAVVVSGAVWRLDAGNWYHSGDFVPDIRVGEHTVSF
                     TNIPLWLTPASLIVQITTDLTTVTGLYTQVTGIHVDMLPPEAVASGAQWRVAGMPWVT
                     SNATLELAPGDYTVEFHDLPFWGQAPDVKTTVTNGSTTMIAGHYWPMEVYGPPGDTNA
                     ALTLTLLNPRGLAFDPLRRLYIADSDHHRLVILDTRDYSVTNLGVQGAAAGQFSQPFG
                     VTVDRATNLYVADTGNNRIQRRSPAGTWTTWGGTSPGSAVGQFNAPYDVAVDAFTNLY
                     VADRSNYRVQKRTPANVWSIFVSNGVEDAFVRLTKGVTVDSSNRLVVSDYDAGSDYSR
                     IQIFATNQTFLGRIGSSTNSEGGLKRAQRMAYGLTNDLYVADMNNSRVVRRNEPGAWM
                     TLLDDNVLDHPEGVAWDARGYLYIADTAHHRIVRIQVYQSPNVPPAIGSAVPGPGGLV
                     IHWTGAAGWFYAVQYSNDMVNWITLPDASALPGVDGPMSFTDTGFSGLAYRVMAY"
     gene            8702..>9159
                     /locus_tag="PK634_11655"
     CDS             8702..>9159
                     /locus_tag="PK634_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98432.1"
                     /translation="MLSQWTMAVLLAAWAGPATAQDEAPPPEIPAAPEAVAEPEAAPA
                     PETPAPEPAPAEPAPAAQEEAAASTSEVELVELVKERETPAVESTSGELVTITLDNVP
                     IQDVIRMFTRISGANIVSGKDLKGNVTVSLKDVAWEPALRIILDSVNMALV"
BASE COUNT         1520 a         3181 c         3175 g         1283 t
ORIGIN      
        1 ttcctgcgcc agctgtccct ctccaaggaa ttcatcgagc ggcgcgagga atgccaggtg
       61 gagaagctgt acgtctccgg cggcatgagc ctgtcccggt tctggatcag cgacatgaag
      121 caggcctccg gcctggacgt cgagcgctgg aacccgttcg acgggctcca cgcgtcgccg
      181 gacgcgctgc ccgccgcgct ggcggggcag gagacccgtt tcacggcggc gatcggggcc
      241 tgcgtggggg cgtgggagtg accatgacgg cgcgcatcaa cctgctgaag acaacggagt
      301 accggcggca ggggctggtg agcacagcct tcatcatccg cgtgtccatc atgacggtgg
      361 tcctgctggc gctggtgttc ggcgcgctgg ccttcctcca gcgccgcaac gcccggcaga
      421 cgctggactc ggcccgggag ctgtggcgcg tgcgggagcc gctgtaccgc aagacgctgg
      481 cgatgaagca ggacttggcc accaagcgga agctggacca ggaactgcgc ggctggtcgg
      541 cctcgcgcat cgcctggaac gagccgctgg ccgagttcca ggagatcacg ctgcccaccc
      601 tccagttccg ccggctcaac gtccggggcg agatggacgt ccgcgtcccc aaggtcaaaa
      661 ccccgaaggc gggctcggcg gaggccaagc ccgcggaggc cgccgccccg gcgctggcgg
      721 cggggacgcc cgcccgacgg ttcttcctgc tgatcgaggg ccgcgcggag ggggaccagg
      781 ccgaggacgc cgtgctgcag ttcgacgcgg cgctccggcg ggcgcccggc ctgcgcgccc
      841 tgatggaatc cgtgcgcctc caaaggctgg agagcgagga gagccgcgcc gggggggcgg
      901 cgggccaggt attcgcgatc gaggccgcga ccgcgcggcg ggagatgccc tgaccatggc
      961 cacccttaaa aaggagcaga tgcaactgat cttcctggcc gggatctgga tcgtgggcgg
     1021 cctcatcgcg ctgtacgtct gggtggtcgg cccggccctg cggaaccgga gccggagcac
     1081 aggcgaactg gatacgctca acgagaacat ccagaaagcg aagctggccg tgcagggcga
     1141 gtccaagctg cggaaggaat acgaccaggc catcgcggag atccggcagg ccatggagca
     1201 gtacatcgtt ccgccggaga gcgccctctc ctgggtgacg gagaaggtgt acgcgaccgc
     1261 gcgcacggtg ggcgtggaag tgcggagcgt gacgccggtc cggatgcccg acaccgcgtg
     1321 ggacggcctg gtcaagtcgg gccgcttcgt gcgcccctac gccgttcagc tcacgctgga
     1381 gtgcggctac gagaagctgc gggccctggt tcgcgccttt gaaacgggaa atccctacct
     1441 gtgcgtcacc ggcctcaacg tcctgggcca gccggatcag agcccggccc ggcaccaggt
     1501 catgttgcag atcgaatggc ccatgtgggg ccggccgctc gggatcgaac tgccggggga
     1561 ggaagaggaa ccgccggcga agttggaggc ggagaatcgc ggatgacgcg ggttcgcgcg
     1621 aatttttaaa acgacagcag atggcgcggg gatacaaccg aggtgagccc atgaggacga
     1681 agcatgcagc gtggcgacgg aactttcttt tcgcaatggc ggtcctggcc gggccgggct
     1741 gcgaaaccga cgacaacgtg gacagcggcg ggatcgccgg ctacttcgaa aacaatccct
     1801 acacgtcgct cgagcggctc gaggggtacg agtacttgag gatcacgccg gcgtcggccg
     1861 aggtgacctt cgtgggccag accgtcctct tccaggtgga cggcgggacg ccgccgtacg
     1921 agtggagcgt cgggctgccg ggcgtggggt ccgtcggctc ggtccgggag aacgaggcgg
     1981 tctacacggc gggggcgatc gcggccaaca acgtgctggt cttcgacgtc gcgggccggt
     2041 ccggcgtggc ggcgatcaac ggcgggatcg agacggagac gctggcggtg aacgccgatc
     2101 ccgacacgct ggccgtggac ggcgatcgca ccgcggtgaa cgtcagcggc ggcgtgccgc
     2161 cctaccgctg gaccgtcctc gacggcgacc tgggctccat cgaccgcagc agcggggaga
     2221 gcgtggtcta cacccggctc cggtccggcg acaactccgt gcgcgtgacg gacagcgagg
     2281 gcagcaccgg cgcggtcgtg atccagcagc ccgcctccgg gggcgcgctc accgcctccg
     2341 ccagcccgtc gagcctgccc aacaacggcg accgcgcgac cctgacggcc agcggcggca
     2401 cgccgccgta ccggtggtac ctgcaggacg ccgccctggg cagcctggac accgacggcg
     2461 gctccagcgt gacctacacg cgcaaccgcc cgggcgacaa ctccgttcgc gtgaccgacg
     2521 ccaacgacga caccgtgacc gtgctgattc agcagccgta aggagaacgc catgaaagcc
     2581 cgccgcatca tgcttctggg ggccgcggcg ctgggcctgg gattccacgc cgggcccgcc
     2641 cgtggacagg tgctgttccc gaccagcggg aacgtcacgg ccggcgacac gttcccgttc
     2701 ctcgtcacct tcctgaacga ggacacgaac gatccgccca ccaagatcta catgttctcc
     2761 gaggacccgg accgcctgtc cgtgccgtcg caacccgtga cgcaggccac gctctacaac
     2821 ccggccacct ccaacttcta ctaccggtcc tggctggacg gcgcggaggg cctgatcgcg
     2881 gcggggctca agccggtcga cggcctcttt tccccgatcc gcgtgcactt cgtcggcgtg
     2941 cagtacggca tcgtgtggtc ggactatgcc gatatcgtcg tcgatccccc gacgttcgtg
     3001 taccagcccg cggcccccgc cctgcgcgtg ggcgagacgg tgccgctggc gatccggcgg
     3061 acgatcgacg ccaacgcgac gctgaacttc caggtccaga gcgcctggcc ggcggtcgtc
     3121 ggcctgggga cgaacgccgt cggcctgcag accaacgcga gcctgaacct gcagttcggc
     3181 gtgttcgaga tcagcaagac gatctacgta tccggcctgg ccgccgcgca ggacgccggc
     3241 tcgacgaacg tgacggtgac ggcgcgcgtg ggcagctacg cgaccaacgt cgtctgcctg
     3301 gtgagcacga acgcgggcct gatcctcgcc cccgcgtcgc cgtccgtcgg cgcgggcgac
     3361 gccgtcgcca tgaccgtgac gcggccctcg gccaccaacg tgtccgcgct gacggtgtat
     3421 ctcaccagcg acgacccgac ggtggtcggc gtgccggcca gcgtggtgat cccgcagggc
     3481 gcgcagagcg tcggcttctc cgcgaccggc ctccggccgg gctcgtcgtt cgtccgcgcc
     3541 acggccatcg gcgtggagcc ggggccgggc agcgcgcgcg aggtggtcgt cggcaatccg
     3601 ctgctggcct tcgacccgga cccggccacg gtgccgcagg ccgagctccg ggtcatcacc
     3661 gtgcgccggc cggcgatgga ggccggcggc tcgctcacgg tgccgctcgt taacaatgcg
     3721 ccgacgctgt tcgaattgtc ggccacgaac gtcactatcc cggcgggctt tgccgacacg
     3781 acgttcacga tcctgggtct ttccgagggg agcggcagcc tgtccgcccg atcgggagga
     3841 tacctgacga acctggcggt gaacgtcacg gccgccacga acgaggacga tcccgacggc
     3901 gacgggatcc cgaccgactg ggaactggtc ctgggctccg acccgtacga cgccttctcg
     3961 cttgatcccg ggctcaccct gaacgacggc gagtgggatt ccgacggcga ggggttgagc
     4021 aactacgagg agatcatcaa ctacgcgacc gatccgctgc ggggcgacac ggacgacgac
     4081 ggcgtggacg accgcaccga ggtccgcgac gacatcacca gcccgctgca cccgatgagc
     4141 agcgtgctgt accgcgagcg gagcctcgac ctgggggcgg tgccggccgg cgggatcagg
     4201 ctacccgatc cctcgcgctt ctacgtccga accaacggct ggacggtgga atgctggctg
     4261 cggccgacca cggacggcag cgggcgggtg tttgcgctgg agggcgcggg ggcgggccag
     4321 agcttccggg tgagcctgga ggacttccgg ccgaaagcgg agattctctc cggggccagc
     4381 gtgatcgcct cggccggcgg ggttggcccg gcggggtcca tccagcaact cccgacaaac
     4441 gagtggtcgc acgtggcctg ggcgtggggg ccgcaggaca acagcctgaa gatttacgtc
     4501 aacggcgttc tgctgatcgc gcaggagacc ctggtgtctc cggacttctc ggcgggcacc
     4561 ggcgtcctgg cgcaggcgtt cacggacggc tatctcgacg acgcgcggtt ctggaattat
     4621 gaccggagct gggaggactt ggattactgg aacaaccgct tctacccctc gccggaaggc
     4681 tacgtccgcc cgccgaccta cgggcagtcc ctgcggatgt actaccgatt cgacgacggc
     4741 gggtcgaaca ccgtggactt cgccttccta aaccagccgg cctatttcct tcccgacacg
     4801 gcgacgatgg tcgtgaccaa ccccgcggtc tcgctgctgg gccgggacga cgaggacggc
     4861 gacctgctgc ccgagtggtg ggtcacgatg cacaacctcg accagtaccc ggaattcgat
     4921 tacggcccga agttcgagta catcgaggtg cctggcaact gggcgcgcat ttcctatttc
     4981 cgcacgttcc gcgcctacac ctcggtcggg aacgagacag gctgggcgca gcctccggac
     5041 aacctctacc acgagcccaa ggaccagggg ctggggcacg acggccgcca ctcggcgttc
     5101 atgaagtacg tgtacctcta cagcgcgccc cggagcgcga ccctcgagat cttcaccccc
     5161 ggcatggagt ccacgctcgt gtatgtcaac ggggagctcc tgacgccgac caacgacacg
     5221 gccaacacgg cacagagcct ggacgtcgcg ggccgtctga agatcgggcg caacatggtg
     5281 tacgtccgct gcatcagcag cttcgacacc tggctggacg aggcccggac gcagccggtc
     5341 aaggtcaccg acccgccggt gcagtacgag cgggcgctcg gaaagttcga cgcccggctg
     5401 acggtggacg gcatcgagca catcgtccgc ggcgacacga gccgaaacga tccgcgcgcc
     5461 gcatggttcg tccagacctg gtccacgttc tggcagatga tcaacgggga cgagcacgcc
     5521 gcccggccgg acaaggagat ccggttcctg ccgggcaacc cggactatgg cctgccgttc
     5581 gacgccgaca ccgacgactt caacgcgtgg tacgagttcc tctgcggcgc gaatccgcgg
     5641 gacgacgatt cgaacaacaa cggcatcccg gacggcctgg aggacttcga cggcgacggc
     5701 ctggtcaacc gcggcgagca ggagcgcggc acccatcccc tgctgcccga caccgacgac
     5761 gaccggcgcg tcgacggcgc cgaggtgggc gcgggcgagg accccgccag cgcgcagagc
     5821 ccggccatct cgcgcgccct ttcgttctcg accaacggct ccgactacgt cgagatgccc
     5881 atgcagcggc ggttcgccct ggacagctgg acgctggaaa gctgggtccg gctggccccc
     5941 ggcgaggccg acggcggcgt gctggtccag cgcgtggtcg gcccgagcgg cgtgaactac
     6001 gaactgggcc tgggcgacgg cgtgaccgcg cccgccaacg tcccgtacgt gcgctacgtg
     6061 tccgtggacg ggttcaccgt gcaggcgacc ggcagcgtcg ccctggccgg cggcaccaac
     6121 tggacgcacg tggcgggcac ctacgacgga ttcctccgcg agctgcagct gtacaccggg
     6181 ggcgtgtacg tcgccacggc gccccaggcg ctccagtccc cggccatcta cgccggcggc
     6241 cccgtgcagc agcgcatcgg cccgggcctg cagggcgccg tggacgacgt ccgcatctgg
     6301 agcgtcgccc gcaccgcctc ggagatcgcc tcgtccttcg acagcgccct ccagggcagc
     6361 gagccgaacc tggtcgccta ctaccgcgcc gacgacagca ccagctaccg caccaacccg
     6421 ctcgtcggga ccagcgccaa caacgggacc aacgcctccg tgaacgtcat gccctggacc
     6481 tggggccagg tccaggatta cgtgcgcggc tactccggcg actggtggga caagtggaac
     6541 cacgccgcca cgctgcgcgg ccgggccggg cacacggccg acggggacgg cgccctgatc
     6601 atcccgccct cgctgcgcgt cacgctgctg cccccgcccg ccgtcgacga cggcgcccag
     6661 tgggccatcc agggcctcgg ctcgtggcgc gacagcgggg tcaccctgta cgaagggctc
     6721 gacgagggtc cgcaggtcat tctcttcaag tccatcgacg gctggacggc cccatccaac
     6781 cagacggtga cgctctccaa cggcgtgctg acctccgtca cggccacgta catccagaac
     6841 ggctcgctgc gcgtggagtt cagcttcgtg gacgaggcca cgcacggcgc catctggccg
     6901 acggacggcg cccagtggcg gatcggcagc ggggtgtggc acgaggagaa cgatacggtg
     6961 gacaacctga ccccgggcga gtacatcgtg cagttcaagt ccatcgacgg ctgggtgccg
     7021 cccgcgccga gcccggtgac gatcctctcc ggcgagacca cgctgctgct gggccagtac
     7081 gtgcccgcga cgggctcgct gcgggtgttc atcgagccgg aggccgtggt ggtctcgggc
     7141 gccgtgtgga ggctggacgc cggcaactgg tatcacagcg gcgacttcgt gcccgacatc
     7201 cgcgtgggcg agcacaccgt cagcttcacg aacatcccgc tctggctgac gccggccagc
     7261 ctcatcgtgc agatcacgac ggacctgacg accgtcaccg gcctgtacac gcaggtcacc
     7321 ggcatccacg tggacatgct gccgccggag gccgttgcct cgggcgccca gtggcgggtc
     7381 gccggcatgc cgtgggtcac ctccaacgcc acgctggaac tcgcgcccgg cgattacacc
     7441 gtggaattcc acgacctgcc gttctggggc caggcgcccg acgtgaagac tacggtcacg
     7501 aacgggtcga cgacgatgat cgcggggcac tactggccca tggaagtgta cggcccgcct
     7561 ggcgacacga acgcggcgct gacgctcacc ctgctgaatc cccgcgggct ggccttcgat
     7621 ccgctccgcc ggctgtacat cgcggacagc gaccaccacc gcctcgtcat cctggacacg
     7681 cgggactaca gcgtgaccaa cctcggcgtc cagggcgccg ccgccgggca gttcagccag
     7741 cctttcggcg tgacggtgga ccgcgcgact aacctgtacg tggcggacac gggcaacaac
     7801 cgcatccagc ggcgctcccc ggcggggacg tggaccacgt ggggcggcac gtcgcccggc
     7861 agcgccgtgg gccagttcaa cgcgccgtac gacgtcgcgg tggacgcgtt caccaacctg
     7921 tacgtcgccg accggagcaa ctaccgggtc cagaagcgca cgccggcgaa cgtctggtcc
     7981 atcttcgtct ccaacggagt ggaggacgcg ttcgtgcggc tgaccaaggg cgtcacggtg
     8041 gattcgtcca accggctggt ggtgtccgac tacgacgcgg gctccgatta cagccgcatc
     8101 cagatcttcg cgacgaacca gacgttcctg gggcgcatcg gcagcagcac gaacagcgag
     8161 ggcggcctga agcgcgcgca gcgcatggcg tacggcctga ccaacgacct gtacgtggcc
     8221 gacatgaaca acagccgcgt ggtgcggcgg aacgagccgg gcgcctggat gacgctcctg
     8281 gacgacaacg tgctggatca cccggagggc gtcgcctggg acgcccgcgg ctacctgtac
     8341 atcgcggaca cggcgcacca ccgcatcgtg cgcatccagg tctaccagtc ccccaacgtc
     8401 ccccccgcta tcggctccgc cgtgcccggg cccggcggcc tggtcatcca ctggaccggc
     8461 gccgccggct ggttctacgc cgtgcagtac tccaacgaca tggtgaactg gatcaccctg
     8521 ccggacgcct cggcgctgcc gggggtggac gggccgatga gctttaccga cacgggattc
     8581 agcggattgg cctaccgggt gatggcctat tgagacgagg tgggaggcgg acaggcggcg
     8641 cggcttgaag aggaacgcgc tttgaggtaa ttacgaaggg accggcacta tgaagaaacg
     8701 aatgctctct caatggacga tggcggtcct cctcgccgcg tgggcgggcc cggcgacggc
     8761 ccaggacgag gcccctcccc ccgaaatccc ggccgctccg gaagccgtcg ccgaaccgga
     8821 ggccgccccc gcgcccgaga cgccggcccc ggagccggcc ccggcggaac ccgcgccggc
     8881 cgcgcaggaa gaggcggcgg cctccacgtc cgaagtcgaa ctggtggaac tcgtcaagga
     8941 gcgagagacc ccggcggtgg agtcgacgtc gggcgaactc gtcacgatca cgctcgacaa
     9001 cgtgccgatc caggacgtca tccgcatgtt cacccgcatc tcgggcgcga acatcgtctc
     9061 gggcaaggac ctcaagggga acgtgacggt cagcctgaag gacgtggcct gggaacccgc
     9121 cctccggatc atcctcgaca gcgtcaacat ggcgctggt
//
LOCUS       DAOOTK010000120         9058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4882_length_9058_cov_4.693658, whole genome shotgun sequence.
ACCESSION   DAOOTK010000120 DAOOTK010000000
VERSION     DAOOTK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_9058_cov_4.693658"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK634_11660"
     CDS             complement(<1..453)
                     /locus_tag="PK634_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98433.1"
                     /translation="MTPVQVTLVLTLALNLALGVFVLATQPGRATNRVFFMLSLVLAA
                     WLVGVLFITMAASARWASFWIRQAYAVALLIPMAGDYLRQAVVDHGTRRLPRIRGGWG
                     LWFATVFIAGLCQTGYFLAGVQMPGAGQGLPYAVYGPGLHVFTVYYLAA"
     gene            complement(450..1694)
                     /locus_tag="PK634_11665"
     CDS             complement(450..1694)
                     /locus_tag="PK634_11665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOW98434.1"
                     /translation="MRAPQRVVVTGIGVVAPNGIGKDAFWASLLEGRSGVGPITLFDA
                     TDTPCKAAGEVRDFKPTDFIDPAMKPRRMSRCTQLAVAAARLALEDSGLEAAQLARHF
                     PMPLVMGVSTSAPDMWEQHHLQIEKRGAANGPPYIIGAFMPHAAVNAISACLGLQTQP
                     LTMSSACAAGMDAVGAATEQIQSGRADVAMAGGADAPISYTAFTSFAACGLISADVTD
                     PRTASRPFDRRRVCGVISEGAAVVVLENMNHARARGARPYLEIKGYAMNADPDPGLPG
                     SGLAGTMKMALANAGRHAARVDYVNAHGPGHPVLDRVETNMIKAALGQRASRIPVSSI
                     KGHIGNPLAAGGVLQLAACALIIRHRRIPPTANLEEPDPACDLDYVAGRPRAAVLDCV
                     VINSHGLGGRNGSMVVERIAPE"
     gene            1938..3020
                     /locus_tag="PK634_11670"
     CDS             1938..3020
                     /locus_tag="PK634_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOW98435.1"
                     /translation="MSLFRKPIRHWIQRLLENTILKKPSENIYRILGGHIYFQTMATA
                     VRLDLFSLLAQRGKMTLPQIAEAVGIQEKPARIVLLGCTALGLLRKRGDRYRNTWLAG
                     RLLDRARPDNIIPVVLWQHFINYRAMAHFYEAARSGSHTGLSEFQGPETTLYGRLTHH
                     PELEKIFQDAMESISVQSNRLLAEAVDFSRFQHILDVGGGNGSNIIQLARRHPSLRAT
                     VFDSHSVCVLARAHIRDAGLADRLDSIAGDAFQDEFPAGVDGILFAHFMTIWSEAKNR
                     ELLRKAHRALPSGGAAIIFNMMQHDDGTGPLSAAMGSPYFLTLATGEGMLYTCKEYAG
                     WMRDAGFSNVFVHRLVRDHGVILGIK"
     gene            3240..4511
                     /locus_tag="PK634_11675"
     CDS             3240..4511
                     /locus_tag="PK634_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOW98436.1"
                     /translation="MKTLKTPLLGRIAMLIVLIVAAGTRPSGASGFRNPPDGASALGH
                     VGGKMTLTRDASLLSHNPAGLTELGRAEFLASCTLADGDAEFDSAVPPGGHAETENDP
                     QWLPAVYAVAPLDSGRLIAGVGITSPYGQSTEWSQGSLFRYASPYFAELRTLDVSPAV
                     AVQITDSFSIGLAADILFSDFELRQVYPWSQAMGIPLLPDGEARFDADGTGLGAHAGL
                     QWKCTPRQVLALAYHAPFDIDYEGDFSVSGAPAGGGLPISSSPFDTTVRFPSVVAAGY
                     GFQASDTIRLGAEVEWIEFSRYDELVLNAGLLNALLPQTVIPEQWKDSWTAGLGADWQ
                     VSERWVLRAGYVFIESPIPDSTLAPSLPDGDRHVLSLGAGYVRTRHRLDAAFAYSLFE
                     DRHVDANPNPLFNGAYELSSLLFAFSYGYLF"
     gene            4526..5665
                     /locus_tag="PK634_11680"
     CDS             4526..5665
                     /locus_tag="PK634_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW98437.1"
                     /translation="MQGSDSAVLVVDDENGPRESLRILLKNEFRVLCADSVDTATTLL
                     AQERPSAIILDIRMPGKNGIDGLREMRGLDQDVSIILLTGYSTLETAQEAVRLGANDY
                     IKKPFDASEMLDVVRHGVLRTELERRRRKAEEQLNRLNRNLQERIIQSDPLVSMGSRA
                     AELAHDIRNPLTLVLGYVELLSVQLREAGTILGDKWPATRSYLEMIETSVLRCKEMAD
                     TWTSEGRHSRMSRGPVDILALVMDLASTMTAWAQIHGAEIRVRSDAHDLRVNALRLQL
                     LRAVQNLMVNAVDAVRGKKGGLVEVRVRREKDNAIIEVADNGPGITAKTQQNALAAPT
                     SEPCGLPGMGLRIVRQIVEEHGGRLMLASQPGEGAIATLQLPLLS"
     gene            5722..7335
                     /locus_tag="PK634_11685"
     CDS             5722..7335
                     /locus_tag="PK634_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOW98438.1"
                     /translation="MIMDDFSAALHEWREILGAEQVLDTPADRAAYEASVSGFRRFIP
                     AILRPMDTAGVQRIVAVANARRIPLYPVSRGCNWGLGSRLPARDGAVIVDLARMNRIR
                     EVNERFHYAVVEPGVTQRQLYDHLRERGLPLQLNVTGSAADTSLIGNALERGIGYFSS
                     KADSLAALEIVLGNGTLLRTGFGHLTAAKTPHLYRHGIGPALDGLFFQSNYGIVTGAT
                     VDLRPEPEAGMVLIARLGEEERLPAFVEALVDLRRDGMFSTVAHIGNRHRTRIAMAPL
                     VHRELSGWMRGEDSELRTRAEQLLEEQGFGPWSAVVGIPGLPGQLCEARRRIRRRLRG
                     LARLLFLTDRLVAWADRLSYRLRWIPWVRQQRALLKAAEPLYGLARGIPTDAPMLSVA
                     WPLGKRAASERDNPDAGDCGLLYCVPFLPADGGLVREAMNLAEGVFSKHAFTPFITLN
                     LVDDRAVECVINLAFDRTSTERTAAAHACNDELTAEFIRRGFPPYRVGSQSMDLILDE
                     ADPFWQTARDLKCALDPNGIIAPGRYNLR"
     gene            complement(7339..8868)
                     /gene="glgA"
                     /locus_tag="PK634_11690"
     CDS             complement(7339..8868)
                     /gene="glgA"
                     /locus_tag="PK634_11690"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOW98439.1"
                     /translation="MPKARTRKPARRPSPPAPGPGLKILFVASECAPYAKAGGLADVV
                     AALPKTLRQMGHDARIVMPLYAAIDRARYGIGFLQPVCVHMGNGEEQWIGLHGGRLDG
                     EVPVWFVDCDRYFGRPGLYDEAGGEYGDNAFRFALLSKAATQICKDTGFIPDVMHAHD
                     WPAALAPVFLKTWDRILSPLSATASVLTIHNIGYQGVYHASVFPYIGVGWEHFVPERF
                     EDHGNVNLLKAGVAFADALTTVSPAHAREILEPIGGMGLAPHLDARRADLTGILNGTD
                     YDHWNPETDPFIPAHYSLKDLSGKAACKADLQKRMGLAERPDWPLFGIVSRFAPQKGF
                     GLLAECLPDALRQMLFQVVVLGTGDPWMENVFRGLAAAWPGRVACHIGFSNELSHWIE
                     AGSDFFLMPSLYEPCGLNQMYSMKYGTLPVVRATGGLDDTVENYNEKTGLGTGFKFWE
                     PAAAAVCDTVGWAVSTWFDRPHHIAKLRRNAMQQNFSWETSAAEYVRVYEHAVAARRR
                     G"
     gene            complement(8877..>9058)
                     /locus_tag="PK634_11695"
     CDS             complement(8877..>9058)
                     /locus_tag="PK634_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOW98440.1"
                     /translation="GNRYIYMEVGHAGQNASLEAVALGLGAVVVAAFSDADMRKALGL
                     PKGEDPFYLMPVGRK"
BASE COUNT         1572 a         2961 c         2952 g         1573 t
ORIGIN      
        1 ggccgccaga tagtacacgg tgaacacatg gagacccggc ccgtagacgg catacggcag
       61 tccctggccg gcgcccggca tctgcacccc ggcaagaaag tacccggtct ggcacagccc
      121 ggcgatgaaa accgtggcga accacagccc ccagccgccc cggatgcggg ggagacggcg
      181 cgtcccgtga tcgaccacgg cctgccgcag gtaatcgccc gccatcggga tcagcagcgc
      241 cacggcgtag gcctgccgga tccagaacga cgcccagcgg gccgaggcgg ccatggtgat
      301 gaacagtacg ccgaccagcc acgccgccag caccaggctc aacatgaaga aaacgcggtt
      361 cgtcgcgcgc ccgggctggg tcgcgagcac gaacaccccg agcgccaggt tcagcgccag
      421 cgtcaggacc agggtgacct gcaccggggt cattccggcg cgatcctttc aacgaccatg
      481 ctgccgttgc gtccaccgag cccgtggctg ttgatcacca cgcaatcgag cacggcggcc
      541 cgcggccggc cggcgacata atccaggtcg caggcgggat cgggttcctc cagattcgcc
      601 gtcgggggga tccggcggtg ccggatgatc agcgcgcagg ccgccaactg cagcacgccg
      661 ccggcggcca gcgggtttcc gatgtgcccc ttgatggacg acaccggtat ccggctggcc
      721 cgctgtccca gcgcggcctt gatcatgttc gtttcgacgc gatccagcac gggatgcccc
      781 ggcccgtgcg cgttgacata gtccacgcgc gcggcatggc gaccggcatt ggccagcgcc
      841 atcttcatgg ttccggccag tcccgaaccg ggcaagcccg ggtcgggatc ggcattcatg
      901 gcgtatccct tgatctcaag atagggacgg gcgccgcgag cccgggcatg gttcatgttt
      961 tccagcacga ccaccgcggc cccctcggag atcacgccgc atacgcgccg acgatcgaaa
     1021 ggccggcttg ccgtgcgcgg atccgtcacg tccgcggaaa tgagcccgca agcggcaaaa
     1081 ctggtaaagg ccgtgtagct gatcggggca tccgccccgc cggccatggc cacgtcggcg
     1141 cggccgctct ggatctgctc cgtggcggct ccgaccgcgt ccatgccggc cgcgcaggcg
     1201 ctggacatgg tcaggggctg tgtttgcagc cccaggcacg cgctgatggc gttgacggcc
     1261 gcgtgcggca tgaacgcgcc gatgatgtac ggcggcccgt tggccgcccc ccttttttca
     1321 atctggaggt gatgctgctc ccacatgtcg ggggcgcttg tgctcacccc catcaccagc
     1381 ggcatcggaa aatgccgggc cagttgcgcg gcctccaatc ccgaatcctc caacgccaac
     1441 ctcgccgcgg cgaccgccaa ctgcgtgcag cgcgacatgc gtctcggctt cattgccgga
     1501 tcgatgaagt ccgtcggttt gaaatcccgg acttcaccgg cggccttgca tggggtgtcc
     1561 gtcgcatcaa acagcgtgat gggccccacg ccgctacgac cctccagcag gctggcccag
     1621 aatgcgtctt tgccgattcc gttcggcgcc accacgccga ttccagtcac cacaactcgc
     1681 tggggcgccc tcatgacacg ctcatttcaa aaaagacggt taggctggcc ttgaaatgag
     1741 tcatacttgt caccgtgcgg tgataaagcg gatattgaca atgaacattt atgtacatcc
     1801 ggtcaatcac aattgaaggt ccttgtactc ttttgttcaa tacgtttcag cttgcataat
     1861 aggttactat agaggaaacg tttcttgctc tactcaagtg tgttaagtaa aaagcaaatg
     1921 tttcagagaa accgcccatg agcctatttc gcaagccgat tcggcactgg attcagcgtt
     1981 tgctggaaaa cacaatcctc aagaagccgt cggagaatat ttacagaatc ctgggggggc
     2041 atatctactt tcagaccatg gcaaccgccg ttcgtctgga tctgttcagc ctgctcgccc
     2101 agcgcgggaa aatgacgctg ccccagatcg cggaggccgt ggggatacag gagaaaccgg
     2161 cccgcattgt gctgctcggg tgcacggccc tcggcctgct gcggaagcgc ggcgatcgat
     2221 atcgcaacac ctggcttgcg ggccgcctgt tggatcgcgc ccggcccgac aacatcatcc
     2281 ccgtggtgct gtggcaacac ttcatcaact accgggccat ggcgcatttc tacgaggccg
     2341 ctcgaagcgg gtcccatacc ggcctttccg aattccaggg ccccgagacc acgctctacg
     2401 gccggctgac ccatcacccg gagctggaaa aaatattcca ggacgctatg gagagcatct
     2461 cggtgcaatc caaccgactg ctggccgagg ccgtggattt ctcccggttc cagcatatac
     2521 tggacgtcgg agggggcaac ggatcgaata ttattcaact ggcccgccgc catccttcgc
     2581 tgcgcgccac tgtcttcgac tcgcacagcg tgtgcgtact tgcccgggcc catatccggg
     2641 acgcgggact ggcggaccgc ttggactcga tcgcggggga tgcattccag gatgagtttc
     2701 ccgccggcgt ggacgggatt ctctttgcgc acttcatgac catctggtcg gaagctaaaa
     2761 acagggagtt gctgcggaaa gcgcaccgcg ccctgccatc cggcggagca gcgatcatct
     2821 tcaacatgat gcagcacgat gacggaaccg gccccttaag cgccgccatg ggctctccct
     2881 actttctgac cctggccacc ggcgagggca tgctgtacac gtgtaaggaa tatgccggct
     2941 ggatgaggga cgccggtttc tccaatgttt tcgttcatcg cctggttcgc gatcacgggg
     3001 tgatcctggg catcaaatag agactgcgct gtacatattt gcgcagatat ggaggccgag
     3061 tatcaaccgc gagcggacag gattgttttc ttgccccatc ttgtagggga aatcctgcgg
     3121 ctgtcttttc gaaaggcgcg gctggcacgt aacttgctat ccaacgcacc gttgcgagcg
     3181 cggggacagg ccatcggcaa cgaaccaagg acagcataag tgacccgaaa ggaatatgca
     3241 tgaaaacact gaagacgcct ctgctgggcc ggatcgccat gctgatcgtt ctgatcgtag
     3301 ccgccgggac gcgcccttca ggggccagcg gattccggaa tccgccggac ggcgcgtccg
     3361 ccctcggaca cgtcgggggc aaaatgacgc tgacgcgaga cgcctcgcta ctttcgcata
     3421 atccggcggg attgaccgag ctgggccgcg ccgagttctt ggcttcctgc acgctggcgg
     3481 atggcgacgc ggaattcgat tccgccgtgc cgccgggcgg ccacgccgaa acggagaacg
     3541 atccgcaatg gctgcccgcc gtatacgcgg tcgcgcccct ggactcgggg cgcctgatcg
     3601 ccggcgtggg catcacgtcc ccgtacgggc aatcgaccga atggagccag ggcagcctgt
     3661 tccgctacgc ctcgccctac ttcgcggagc tgcggacatt ggacgtttcg ccagcggtag
     3721 ccgtgcagat cacggactct ttctccatcg gcctggcggc cgacatcctg ttttccgatt
     3781 tcgagctgcg ccaggtatat ccctggtcgc aggccatggg gattcccctg ctgccggacg
     3841 gcgaggcccg gttcgatgcc gacggcacgg ggctgggcgc ccatgccggc ttgcagtgga
     3901 aatgcacgcc tcggcaggtc ctggcgctgg cgtaccatgc gccgttcgac atcgattacg
     3961 agggcgactt ctcggtcagc ggcgctcccg cggggggggg cttgccgatc tcgtccagtc
     4021 cgttcgatac gaccgtccgg ttcccctccg tggtcgcggc gggttacggt ttccaggcca
     4081 gcgacaccat tcgcctgggc gcggaggtgg aatggatcga gttctcgcgg tacgacgagc
     4141 tggtcctgaa cgcgggcttg ctgaacgcgc tgctgccgca gaccgtgatc cccgagcagt
     4201 ggaaggacag ttggacggcc ggcctcggcg cggactggca ggtgtcagaa cgctgggtcc
     4261 tgcgggccgg ttatgtgttc attgaaagcc cgatcccgga ctccaccctg gccccgagcc
     4321 tgcctgacgg ggatcgccac gtgttgagcc tcggcgccgg atatgtgcgg acccgccatc
     4381 gtcttgacgc ggcttttgcc tacagccttt tcgaggaccg ccatgtcgac gccaatccga
     4441 accccctctt caacggcgcc tacgagcttt cgtcgctgct gttcgcattc tcctacgggt
     4501 atcttttcta atggaggcgc ttcacatgca ggggtcggat tcggcggtgc tggttgtgga
     4561 cgatgaaaac ggccctcggg agagcctgcg catcctgttg aagaacgagt tccgggtgct
     4621 gtgcgccgac agcgtggata ccgcaaccac cttgctggcg caggagaggc cttcggctat
     4681 catcctcgac atccgcatgc cggggaaaaa cggcatcgac ggcttgcgcg aaatgcgcgg
     4741 gctggaccag gacgtctcga tcatcctgct gaccggctac agcacgctgg agacagccca
     4801 ggaggcggtc cggctggggg ccaacgacta catcaagaaa ccgttcgacg cgtccgagat
     4861 gctggacgtg gtccggcacg gtgtcctgcg gacggaactg gaacgccggc gccgaaaggc
     4921 cgaggagcag ctgaaccggc tgaatcggaa cctccaggag cgcatcatcc agagcgatcc
     4981 gctggtctcc atgggcagcc gggccgctga actcgcgcac gacatccgca atcccctgac
     5041 actggtgctg ggctacgtgg aacttctctc cgtgcaactg cgcgaagcgg gcaccatcct
     5101 cggcgacaag tggcccgcca cccgctccta cctggagatg atcgagacca gcgtgcttcg
     5161 ctgcaaggaa atggccgaca cctggacgtc cgagggccga cacagccgca tgagccgtgg
     5221 cccggtggat atcctggcgc tggtgatgga cctggcttcc acgatgaccg cgtgggccca
     5281 gatacacggg gccgagatcc gtgttcggag cgacgctcac gatctccgcg tgaacgccct
     5341 gcgacttcag ttgttgcgtg ccgtgcagaa tctcatggtg aacgccgtcg acgccgtacg
     5401 cgggaaaaag ggcgggctgg tggaagtccg ggtgcggagg gagaaagaca acgcgatcat
     5461 cgaagtggcc gacaacgggc cggggataac ggcgaagacg caacagaacg cattggccgc
     5521 gcccacttcc gaaccgtgcg gtctgccggg catgggcttg cgtatcgtgc ggcagatcgt
     5581 ggaggagcac ggtggccggc tgatgcttgc cagccagccc ggggaaggcg cgatagccac
     5641 cctgcagctt ccgctgctct cataaccgcg cggcggagat ccgctggaca accggcagcg
     5701 gattccccat gctgggacca catgatcatg gatgactttt cggcggccct gcacgaatgg
     5761 cgcgaaatcc tgggggccga acaggttctc gacaccccgg ccgatcgagc ggcgtacgag
     5821 gccagcgtca gcgggttccg gcgcttcatc ccggcgattc tccgccccat ggacactgcc
     5881 ggcgtgcagc gcattgtcgc cgtcgccaat gcgcgccgga tccctttgta tcccgtcagc
     5941 cggggatgca actggggcct cggctcccgg ctgccggcgc gggacggcgc cgtgattgta
     6001 gacctggcgc ggatgaaccg catccgcgag gtcaacgagc gctttcacta tgccgtcgtt
     6061 gaaccgggcg taacccagcg gcagctgtac gatcatcttc gtgagcgcgg ccttcccctg
     6121 cagctcaacg tcaccggctc cgcggccgac accagcctca tcggcaacgc cctggaacgc
     6181 ggcatcggct acttctccag caaggcggac agcctcgccg ctctggagat agtgctgggc
     6241 aacggcacgc tcctgcgcac cggtttcggc catctcaccg cggcgaagac cccccatctg
     6301 tatcgccacg ggatcggtcc cgccctggac ggcttgtttt tccagtccaa ttacgggatc
     6361 gtcacgggcg ccactgtgga tctccggccg gaaccggagg ccgggatggt ccttattgcg
     6421 cggctcggtg aggaggagcg gttgccggcc tttgtcgagg cgctggttga tctccgccgc
     6481 gacgggatgt tctccaccgt ggcgcacatc ggtaaccggc accgaacccg catcgccatg
     6541 gcgccgctgg tccaccgcga attgtcgggt tggatgcgtg gcgaggacag cgaattgaga
     6601 acgcgggcgg aacagcttct cgaggagcag ggcttcggac cgtggagcgc cgtcgtcggc
     6661 atccccgggc tgccggggca gttgtgcgag gcgcgccgcc gaatccgccg ccgcttgcgc
     6721 gggctggctc gccttctgtt cctgaccgac cggctggtgg cctgggcgga ccggctttcc
     6781 taccgcctgc ggtggatccc ctgggtgcga cagcagcggg ccctgctgaa ggcggcggag
     6841 ccgctatacg gcctcgcgcg tggaatcccg accgatgccc cgatgttgag cgtggcctgg
     6901 cccctgggaa aacgcgcggc ttccgagaga gacaatcccg atgccgggga ttgcggcctg
     6961 ctctactgtg ttccattcct gccggcggat ggcgggctgg tgcgcgaggc catgaacctt
     7021 gccgagggtg tatttagtaa gcacgcgttc accccgttta tcaccctgaa cctcgtggac
     7081 gaccgtgccg tagaatgcgt aatcaatctt gctttcgacc ggacttccac cgagcggacc
     7141 gctgcagcgc acgcctgcaa cgacgagttg accgccgagt tcatccgccg gggctttcca
     7201 ccctaccggg tgggcagtca gtccatggac ctgatcctcg acgaagcgga tccgttctgg
     7261 cagaccgcgc gcgacctgaa gtgcgcgctc gatccgaacg gtatcatcgc gccgggccgg
     7321 tacaacctgc gctgacggtc agccgcgccg ccgggcggcg acggcgtgct cgtagacccg
     7381 gacgtactcg gcggccgagg tttcccagga aaagttctgc tgcatcgcgt tccgccggag
     7441 cttcgcgatg tggtgcgggc ggtcgaacca ggtcgagacg gcccagccga ccgtatcgca
     7501 gacggccgcc gcggcgggct cccagaactt aaagccggtg cccaagccgg ttttctcgtt
     7561 gtagttttcc acggtgtcgt ccaggccacc ggtcgcgcgc acgaccggga gcgtgccgta
     7621 cttcatcgag tacatctggt tgagcccgca cggctcgtac agggagggca tgaggaagaa
     7681 atcggagccg gcctcgatcc agtggctcaa ctcgttggag aacccgatgt ggcaggcgac
     7741 gcggccgggc caggcggcgg ccaggccgcg gaagacgttc tccatccagg gatcgccggt
     7801 gcccaggacc acgacctgga agagcatctg ccggagggcg tcgggaaggc attccgcgag
     7861 aagcccgaag cccttctggg gggcgaaccg ggacacgatg ccgaagagcg gccagtccgg
     7921 gcgctccgcc aggcccattc gtttctgcag gtcggccttg cacgcggcct tgccggacag
     7981 gtccttcagg gaataatggg ccgggatgaa ggggtccgtc tccgggttcc agtggtcgta
     8041 gtccgttccg ttgaggatcc cggtcaggtc ggcgcgacgc gcgtccaggt gcggcgcgag
     8101 gcccatgccg ccgatgggct ccaggatctc ccgcgcgtgg gccggggaca ccgtggtcag
     8161 cgcgtcggcg aaggcgacgc ccgccttgag caggttgaca ttgccgtggt cctcgaaccg
     8221 ttccggaaca aaatgctccc agccgacgcc gatgtacggg aacacgctgg catggtacac
     8281 gccctggtac ccgatgttgt ggatcgtcag caccgaggcc gtggcggaca gcggcgacag
     8341 gatgcggtcc caggtcttca ggaacacggg cgccagcgcc gcgggccagt cgtgggcgtg
     8401 catcacgtcc gggatgaacc cggtgtcctt gcagatctgc gtcgccgcct tcgagaggag
     8461 cgcgaagcgg aaggcgttgt cgccgtactc cccgcccgcc tcgtcgtaca ggcccggccg
     8521 cccgaagtag cggtcgcaat ccacgaacca gaccgggacc tccccgtcga gccggccgcc
     8581 gtgcaggccg atccactgct cctccccgtt gcccatgtgg acgcacacgg gctggagaaa
     8641 cccgatgccg taccgcgccc ggtcgatcgc ggcgtacagg ggcatcacga tgcgggcgtc
     8701 gtggcccatc tgccggagcg tcttgggcag cgccgcgacc acgtcggcca ggccgccggc
     8761 cttggcatag ggggcgcatt ccgacgcgac gaacaggatc ttcaggcccg gtccgggtgc
     8821 gggcggcgac ggccggcggg cgggcttcct ggtccgggct ttcggcatgg ccatggctat
     8881 ttccggccca cgggcatcag gtagaacggg tcctcgccct tgggcaggcc cagcgccttg
     8941 cgcatgtccg cgtccgagaa cgcggccacg acgacggcgc ccagcccaag ggccacggcc
     9001 tcgagcgaag cattctggcc cgcgtgcccg acttccatgt agatatagcg gttccccc
//
LOCUS       DAOOTK010000121         8950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_4946_length_8950_cov_5.013603, whole genome shotgun sequence.
ACCESSION   DAOOTK010000121 DAOOTK010000000
VERSION     DAOOTK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4946_length_8950_cov_5.013603"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PK634_11700"
     CDS             complement(<1..412)
                     /locus_tag="PK634_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOW98441.1"
                     /translation="MDAFPVEPLLDYVLSGREPERGLPEGVRDMAGRVFHFDGQDSRV
                     VILGGGTGMSTVVGGNAGMPDWGGRPFVGLKQVFPRLQVVVCTTDDGGSTGRLVRRLP
                     MIGIGDLRKSLLSYVRREGLQRAYGLTDSEVLPVV"
     gene            complement(520..1410)
                     /locus_tag="PK634_11705"
     CDS             complement(520..1410)
                     /locus_tag="PK634_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOW98442.1"
                     /translation="MMMKSRNGAQPRPGTERDYQERIQRVLLHLQRHMDEALSLDGLA
                     RVACFSPFHFHRLFTAFVGETPGQHQRRLRIERAASRLRHSREPVTVIALEAGYETPS
                     AFTRAFRQHFGRSPVGFRRMKKPGPPAAAPGAGPLPKEKVMEHRIEECPERRVVFVRR
                     MGRYDKAAGEAWGAVCGFAFPRGLVGRKAEFIGVSYDDPDITAEEKLRYDACITVDRE
                     VNPEGDVGVQTLAGGPYAVFTLKGPYDGLKALYRAIFGQWLPSSGRSLRDVPCFEKYL
                     NHPEQTPPQDLLTEVFIPIK"
     gene            complement(1474..2667)
                     /locus_tag="PK634_11710"
     CDS             complement(1474..2667)
                     /locus_tag="PK634_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOW98443.1"
                     /translation="MADEQVTSGRSGLRGLGRNVIILGVVSLLTDVSSEMLYPVIPLF
                     LTSALGASMTVVGLIEGAAEGAASALKMLFGWISDRTARRKPFVVWGYGLSAVSKPMM
                     ALAGAWPFVLLARLVDRTGKGMRTSPRDALIAASCSAEQRGTAFGLHRAMDTAGAVAG
                     PVLAMLFLSVWGLGYRAMFLLAFIPAALGVLALAFVRAGPAEAGRAAPPRPDRPPLRP
                     ALKRFVAVYGLFALGNSSDVFLLLRAKEAGLGEQGMLLVYVFYNAVYALASTPAGWLS
                     DRFSRRTVMAGGLAVFAAVYLGFALDPAPARIWLLFALYGFYAAAMEGVAKAQVADLS
                     APENRGTAMGLLHSVTGLLAFVASAAAGWLWAEVSPAAPFYYGAACALAAAGLLLLER
                     PGGKA"
     gene            2780..3685
                     /locus_tag="PK634_11715"
     CDS             2780..3685
                     /locus_tag="PK634_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOW98444.1"
                     /translation="MKILLTGAAGLIGSALRPALERDGHEVIPLRRGLPEGGERAGRA
                     WDPGRGWFSPGALEDLDAVVHLAGETIAALRWTAAKKQRIRDSRVAGTRLLCETLARL
                     ARPPRALIAASAIGYYGDRGGEILTEDSGPGRGFFPELCRDWEAATRPAAGAGLRVAN
                     LRFGVVLSRAGGALPRLLPPFQFGLGGPLGRGDQYMSWVALEDAVNIVRRALRDETLR
                     GPVNAASSQPVTNREFTRALGTILGRPTFFRVPAFVLQLVLGEMAGPLLLASARVEPA
                     KLTACGYGFRMPSIEEALANELARR"
     gene            complement(3692..3913)
                     /locus_tag="PK634_11720"
     CDS             complement(3692..3913)
                     /locus_tag="PK634_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98445.1"
                     /translation="MVFAIAALLNTRRRHQAMAQVPPAANPADTRSGTRVTFDVGNTG
                     KQSVFKKVGPNGQPAEPLQTTQEHVYAWE"
     gene            complement(4057..4887)
                     /locus_tag="PK634_11725"
     CDS             complement(4057..4887)
                     /locus_tag="PK634_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOW98446.1"
                     /translation="MYITYWNLKEEPFQNVADTRFAYLSDQHREGLARLTYLALNRKL
                     GGVLTGAYGVGKSMVLRLLATQVHNESKSRFVALDYVPGPVPLFAQQILRQIGCRRVP
                     DAPPDPLEAIRLLNEETPTLGHVVLAVDESQMMTDPETYHFLHLLTNLTLTGRDGQPL
                     SPAFTIILAGYVELDQLRKADESLLQRLQLTWCLQPLNEDQTIEYLQNRIRVAGGDLW
                     IFDLDAMRAVYTATHGLPRVINNVADISLMLGSAARVRQITPEIVAQAVQDVAGAGFM
                     "
     gene            complement(4902..5780)
                     /locus_tag="PK634_11730"
     CDS             complement(4902..5780)
                     /locus_tag="PK634_11730"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOW98447.1"
                     /translation="MIAKTPLGVPFFDAAYGGVYQGRSTLVTGRTNTGKTVMAFQFLR
                     QGLQQDERCLMLSTRQAADLTICADSLGLPISSAVEAENLILLEYSDFVFGMDRTDAM
                     VLPTDGFNQLKEVIETHSIRRVVIDTIVPWVTIQEVERLGQHVLSFARAFDRLGTTTL
                     MTLPKPVSAMAHRLRKILEEIIPICIYLNPSEKSDRLLFQVTKYLGEKKPGPPIEYDI
                     VNALGLAEFTAAPPPSAASSGPAPARKVPPTRPQAPQGGEGPASPRISFSSVMPGLRT
                     DEIKRESGGPAESRGP"
     gene            complement(5777..6166)
                     /locus_tag="PK634_11735"
     CDS             complement(5777..6166)
                     /locus_tag="PK634_11735"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW98448.1"
                     /translation="MAKILVIDDDEQVRNYLNRLFQKLGHQVVLASDGNDGCAQARDP
                     AVQVIVTDLEMAGSMSGMDLVRALRKTRPQCPIVVASGFPTDERLSECEKLGIKDFLT
                     KPFELSFLTAVLKRLLGSENSGAGKVQ"
     gene            6392..7951
                     /locus_tag="PK634_11740"
     CDS             6392..7951
                     /locus_tag="PK634_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOW98449.1"
                     /translation="MSAKDPDHNLRGLLKTDADTPLPVARQQISQLKARYRQTWFYFL
                     LLICMAVAWAFPLRIALLRLLAFRPAHQGVRDQFNFVAIAYESISQKPRDVSPEQFKD
                     HLGGLRKAGYIPISLADVRALYQEGKPLPRKAILVTFDQSRKGAYFEARGPLQRAGWN
                     AVAFLWTEPIIQEDPASLRWPYIRTMLQGGGWEIGVQGHDSYSRIVADSAGTKRNFMS
                     APRWLADENRYESPAEFSARLIADHEAALALIQENLPGKPFAYAFPYGDFGQFDQRAV
                     LSRRLNMDLVGRYYDLGFVLGNTALNTKESDPRRLRRLLVNPRWSTEELIARLDNAWP
                     HPQGYTRQTSLANPAAWVVDWGRAGMGPDFVRFTARTNTTGAKVWLNGSDTYRDFHAR
                     IKVRIELGQFGLFMRATPDGEEHVYLGLGDEGKVWLRQKHAGMKPFTLASGNYVPDAE
                     GNVDLEVFIRDRLFYAQVNGQPLFQNLINTRGQARAGMIGCSVWHPDLGAARARALDF
                     ELEPFRPSAVT"
     gene            8036..>8950
                     /locus_tag="PK634_11745"
     CDS             8036..>8950
                     /locus_tag="PK634_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98450.1"
                     /translation="MVVSTHGLQLQPRWNDRLFSSFASIYRMKFIPEVRLDSFEALGT
                     LSPRALAEEAKSIKADGILLNMGAIMRGMILSQLTSWLQDASQVLAASGLTMLVQLPP
                     VLEQPSTLPSVIQALPEAQVVTTEDATVAVAVEASATNPPVVLKEETAPLPYRDLELT
                     LYYELSGLTQPDETWSTEMHAEWLRQEGRAAFRVGDFQKAREIWTHWKEIEPSNEEPV
                     SLIGDVFLREGDIGKAMDFYRQSLDLNPGQIGLVIRMSRILDVNAKKPEEAMQLLNRY
                     ARIFPNHPDIALAQAEWLIRQRRDEEATA"
BASE COUNT         1453 a         3069 c         3048 g         1380 t
ORIGIN      
        1 agaccacggg cagcacctcg ctgtccgtga gcccgtaggc gcgctgcagg ccctcccggc
       61 ggacgtacga cagcagggac ttgcggaggt cgccgatgcc gatcatgggc aggcgccgga
      121 ccagccggcc cgtcgagccg ccgtcgtccg tggtgcacac gacgacctgc aggcggggga
      181 agacctgctt gaggccgacg aacggacggc cgccccaatc gggcatcccg gcgttcccgc
      241 ccaccacggt ggacatgccc gtgccgccgc ccaggatcac cacgcggctg tcctgcccgt
      301 cgaaatggaa gacgcgcccc gccatgtcgc gcaccccctc ggggaggccg cgctcgggtt
      361 cgcgcccgct caagacataa tccagcaggg gctcgacggg gaaagcgtcc acggacgatc
      421 gatggcccgg tcgcgaggct ctggacgagt ggctcttcat ccgcggggcc catgatagag
      481 cacgggaccc gccggatgaa gttttttccg cgggacaggc tacttgatcg ggatgaacac
      541 ttccgtcagc agatcctgcg gcggcgtctg ctccggatgg ttgaggtact tctcaaagca
      601 gggcacatcc cgcaacgaac gcccgctcga gggcagccac tggccgaaga tggcgcggta
      661 gagggccttg agcccgtcgt acggcccctt cagggtgaag accgcgtacg ggccgccggc
      721 cagcgtctgg acgccgacat cgccctcggg attcacctcg cggtccaccg tgatgcaggc
      781 gtcgtagcgc agcttttcct cggcggtgat atccggatcg tcgtagctca ccccgatgaa
      841 ctccgccttg cggccgacca gcccgcgcgg gaacgcgaag ccgcaaacgg cgccccacgc
      901 ctcccccgcg gccttgtcgt accggcccat ccggcgcacg aacacgacgc ggcgctcggg
      961 acactcctcg atgcgatgct ccatgacctt ctccttcggt agtgggccgg cccccggcgc
     1021 ggccgcgggc ggcccgggtt ttttcatccg ccggaaaccg acggggctcc ggccgaaatg
     1081 ctgccggaac gcgcgcgtga acgccgaggg cgtctcgtac cccgcctcga gcgcgatgac
     1141 cgtcaccggc tcgcgcgaat gccgcaggcg ggaagccgcc ctttcgatcc gcaaccgccg
     1201 ctggtgctgg ccgggggtct ccccgacgaa cgccgtgaag aggcggtgga agtggaaggg
     1261 cgagaagcac gccacgcggg ccaggccgtc cagcgaaagg gcctcgtcca tatgccgctg
     1321 caggtgcagc agcacgcgct ggatccgctc ctggtagtcg cgctccgtgc cgggccgggg
     1381 ttgcgcgccg ttccttgatt tcatcatcat gcccgcagcc tacccttccc cccggccgcc
     1441 cgctttcccg atcttgcggt ttcccgccgg ccttcaggcc ttccctcccg gccgttccag
     1501 caggagcaat ccggccgccg ccagcgcgca ggccgcgccg taatagaacg gcgccgcagg
     1561 actgacctcc gcccacagcc agccagccgc ggcgctcgcg acgaaggcca gcaggccggt
     1621 gacggaatgc aggaggccca tcgccgtgcc ccggttctcc ggcgcggaca ggtccgccac
     1681 ctgcgccttc gccacgccct ccatcgccgc ggcgtagaat ccgtagaggg cgaacaggag
     1741 ccagatacgc gccggcgcag gatccagcgc aaagcccagg tataccgccg cgaagaccgc
     1801 cagtccgccg gccatcaccg tccggcggct gaaccggtcc gagagccagc ccgcgggcgt
     1861 ggaggcgagg gcatagacgg cgttgtagaa gacgtagacc agcagcatcc cctgctcgcc
     1921 cagcccggcc tccttcgcgc gcagcaggag aaacacgtcg ctgctgttgc ccagcgcgaa
     1981 cagcccgtag accgcgacga accttttcag cgcgggccgc agcggcgggc ggtccggcct
     2041 cggcggcgcg gcgcggcccg cctccgccgg cccggcgcga acgaacgcca gcgcgaggac
     2101 tcccagcgcg gccgggatga aggccagcag gaacatggcg cgatagccca gcccccagac
     2161 ggagaggaac agcatggcca gcaccggccc ggccacggcc cccgcggtat ccatcgcgcg
     2221 gtgcaggccg aacgccgtcc cgcgctgttc cgcgctgcac gacgccgcga tcagcgcgtc
     2281 gcgcggcgag gtgcgcatgc ccttccccgt ccggtcgacg agccgggcga gcagcacgaa
     2341 cggccacgcg ccggccaggg ccatcatcgg cttgctgacc gcggagaggc cgtagcccca
     2401 gacgacgaat ggcttccggc gggccgtccg gtccgatatc cagccgaaca gcatcttcag
     2461 cgcgctggcc gcgccttccg ccgcgccctc gatcaggccc acgacggtca tcgacgcgcc
     2521 gagcgccgac gtgaggaaaa gggggatcac cgggtagagc atctcgctgc tgacgtccgt
     2581 cagcaggctc accacgccca ggatgatgac gttgcggccc agtccgcgca gcccgcttcg
     2641 cccggatgtg acttgctcat cggccatgcg tggaacataa cgcttcggct gtcccggcac
     2701 aacctgggcc gacacgcgcc cgttgcgcgc ggagtccgcc ggtcctatat tgggaatacg
     2761 accagccatg ccggagccga tgaaaatact gctgaccggg gccgcggggc tcatcgggtc
     2821 cgccctgcgg cccgccctgg aaagggacgg gcacgaagtc atcccgctgc ggcgcggctt
     2881 gcccgaaggc ggagagcgcg cgggccgcgc ctgggatccc gggcggggct ggttttcccc
     2941 cggcgcgctg gaggacctcg acgccgtcgt tcatcttgcc ggcgagacca tcgcggccct
     3001 ccggtggacg gcggccaaga agcaacgcat ccgggacagc cgcgtcgcgg gcacgcgcct
     3061 gttgtgcgag acgctggccc ggctggcccg tccgccgcgc gcgctgatcg ccgcgtccgc
     3121 catcggatat tacggagacc ggggcgggga aatcctgacc gaggacagcg gccccggccg
     3181 cggcttcttc cccgaactgt gccgggactg ggaggcggcg acgcggcccg cggccggggc
     3241 cggcctccgg gtggcgaacc tccgcttcgg cgtcgtgctg tcgcgcgcgg gcggggccct
     3301 tccccggctg ctcccgccgt tccagtttgg cctgggcggc ccgctggggc gcggcgacca
     3361 gtacatgagc tgggtggcgc tcgaggacgc cgtgaacatc gtgcggcgcg cgctgcgcga
     3421 cgaaaccctg cgcgggccgg tgaacgcggc cagctcgcag cccgtcacca accgcgaatt
     3481 cacgcgggcg ctgggaacta ttttgggtcg tccgaccttt ttccgcgtcc cggctttcgt
     3541 cctgcaactt gtcctgggag agatggccgg gccgctgctc ctggcgagcg cacgggtgga
     3601 gccggcgaag ctcacggcct gcggatacgg attccgaatg ccgagtatcg aggaggccct
     3661 tgcgaatgaa ctggcgcggc gctgaacgcg cctactccca ggcgtagacg tgttcctggg
     3721 tcgtctgcag cggttccgcc ggctggccgt tcgggccgac tttcttgaag accgactgct
     3781 tcccggtgtt ccccacgtcg aaggtgacgc gcgttcccga gcgcgtatcg gcaggattgg
     3841 cggcgggggg cacctgggcc atggcctgat gccgccggcg cgtgttcagc aacgccgcga
     3901 tggcaaacac cacggccaga accgtcgcaa taacgccagt taaagttagg ggtgagtcaa
     3961 acatacaact tcactccagt ttcttaaaag tgaagtctac ccccctcctg ctggaaagta
     4021 aaagcctttt tttgcagcaa aaaaaggccc ccgggctcac ataaaaccgg cgcccgccac
     4081 gtcctggacc gcctgcgcaa cgatctcggg tgtgatttgc cgcacgcggg cggcgctgcc
     4141 gagcatgagg gaaatatccg ccacgttgtt gatgacacgg ggcaggccgt gcgtcgcggt
     4201 ataaacagcc ctcatggcgt ccaggtcgaa tatccataaa tcgcctcccg ccacgcgaat
     4261 acgattctgg agatactcga tggtctgatc ctcgttcagg ggctgcaggc accatgtgag
     4321 ttggagccgt tgcaggaggg actcgtcggc cttacgcaac tggtccagtt caacgtagcc
     4381 ggccaggatg atggtgaacg ccgggctcaa gggctggccg tcccgcccgg tgagcgtcag
     4441 gttggtgagc aggtgcagaa aatgatacgt ctccgggtcg gtcatcatct gggactcgtc
     4501 cacggccagc acgacatgcc ccagcgtggg cgtttcctcg ttcaggaggc gaatggcctc
     4561 cagcggatcc ggcggcgcat cgggaacgcg ccggcacccg atctgccgga gaatctgctg
     4621 cgcgaaaagc ggcaccgggc cgggcacgta gtcgagggcc acgaatcggg acttgctctc
     4681 gttgtggacc tgggtggcca gcaggcggag gaccatggat ttgccgacgc cgtaggcgcc
     4741 ggtcagcacg ccgccgagct tgcggttcaa ggccagatag gtcaggcgcg ccaaaccctc
     4801 gcggtgctgg tcggacaggt acgcgaaacg ggtgtcagcg acattctgga atggctcttc
     4861 cttgagattc cagtaagtga tgtacactcg ttattacgct ttcaggggcc ccgcgattcc
     4921 gccgggccgc cggattcccg cttgatttcg tccgtgcgca ggccgggcat gacgctggag
     4981 aagctgatcc ggggcgaggc cgggccttcg cccccctgcg gcgcctgggg ccgggtcgga
     5041 ggaaccttcc gcgccggcgc cgggcccgac gaggccgcgg agggcggcgg cgcggcggtg
     5101 aactcggcca gcccgagggc gttgacgatg tcatattcga tcggcggccc gggctttttc
     5161 tcgccgaggt acttggtcac ctggaagagc aggcggtcgc ttttctccga gggattcagg
     5221 tagatgcaga tcgggatgat ctcctcgagg atcttgcgca ggcggtgcgc catggccgag
     5281 accggcttgg gcagggtcat gagcgtggtg gtgcccaggc ggtcgaaggc ccgcgcgaag
     5341 gagagcacgt gctgcccgag ccgctcgact tcctggatgg tgacccacgg cacgatggta
     5401 tcgatgacca cccggcggat cgaatgggtc tcgatgactt ccttgagctg gttgaacccg
     5461 tcggtcggca ggaccatggc gtccgtgcgg tccatgccga agacgaagtc gctgtattcc
     5521 aacaggatga gattctccgc ctcgaccgcg ctgctgatcg gcaggccgag cgagtcggcg
     5581 cagatggtga ggtcggccgc ctgccgggtg gacagcatga ggcagcgctc gtcctgctgc
     5641 aggccctgcc ggaggaactg gaaggccatc acggtcttcc cggtgttcgt ccggcccgtc
     5701 acgagcgtgg accgtccctg gtagacgccg ccgtacgcgg catcaaagaa cggcactccc
     5761 aatggcgttt tggcgatcat tgcaccttgc ccgcgccaga gttctcgctg ccgagcagcc
     5821 gcttcagcac ggccgtcagg aaggacagtt caaagggttt ggtcaggaag tccttgatgc
     5881 ccagcttctc gcattcggac aggcgctcgt cggtggggaa gccgctggcc acgacgatcg
     5941 ggcattgcgg ccgggtcttg cgcagggcgc ggacgagatc catcccgctc atggaacccg
     6001 ccatttcgag atcggtgacg atcacctgga cggcggggtc gcgggcctgc gcgcagccgt
     6061 cgttgccgtc ggaggcgagc accacctggt ggcccagctt ctggaagagg cggttcagat
     6121 aattgcgaac ctgctcatcg tcatcgatca cgagaatctt ggccatgtga cgcctccgtt
     6181 tgcctcaacc ggccccccgc gtccatccgc gcggcacggt actcttctcc aatcgatatc
     6241 ctattcatcg gggattgctt gacaagtctt aaaaggattt tttacggccc ggccgggtcg
     6301 ggacggcgcg cgccggccgg gcgctcctcc cctttttgca ctggcgttgc gcggcgattt
     6361 gtcccactat ccgtggcggg cgaggtgccc tttgagcgcg aaggatccgg accataattt
     6421 gcgcgggctg ctgaagacgg acgcggacac cccgctgccc gtcgcccggc agcagatctc
     6481 tcagctcaag gcgcggtacc ggcagacctg gttctacttc ctgctcctca tctgcatggc
     6541 ggtcgcgtgg gcgttcccgc tgcggatcgc gctcctgcgc ctcctggcct tccgtcccgc
     6601 gcaccagggc gtgcgcgacc agttcaactt cgtcgccatc gcctacgaat cgatctccca
     6661 gaagccgcgg gatgtctcgc ccgagcagtt caaggaccat ctggggggcc tgcggaaagc
     6721 cggctacatc ccgatcagcc tggccgacgt gcgcgccctc taccaggagg gtaaacccct
     6781 gccgcgcaag gcgatcctcg tgacgttcga ccagtcgcgc aagggggcct atttcgaggc
     6841 gcgggggccg ctgcagcgcg ccgggtggaa cgcggtggcc ttcctgtgga cggagccgat
     6901 catccaggag gacccggcca gcctccgctg gccgtacatc cggaccatgc tgcagggcgg
     6961 cgggtgggag atcggggtgc aggggcacga cagctactcg cggatcgtgg cggacagcgc
     7021 cgggacgaag cgcaacttca tgagcgcgcc ccgctggctc gcggacgaga accgctacga
     7081 gagcccggcg gaattcagcg cgcggctgat cgcggaccac gaggcggcgc tcgcgctgat
     7141 ccaggagaac ctgccgggca agccgttcgc ctacgccttt ccgtacggcg acttcggcca
     7201 gttcgaccag cgcgccgtgc tgtcccgccg cctgaacatg gaccttgtgg gccgctacta
     7261 cgacctgggg ttcgtcctcg gcaacacggc gctaaacacg aaggaaagcg acccgcgccg
     7321 cctgcggcgc ctgctggtca acccgcggtg gagcacggag gagctgatcg cccggctcga
     7381 caacgcgtgg ccccatccgc agggctacac gcggcagacg tcgctggcga atcccgccgc
     7441 ctgggtcgtg gactgggggc gcgccgggat gggcccggac ttcgtgcgct tcaccgcgcg
     7501 gacgaacacg acgggcgcga aggtgtggct gaacggcagc gacacgtacc gggatttcca
     7561 cgcgcggatc aaggtgcgca tcgagctcgg gcagttcggg ctgttcatgc gggcgacgcc
     7621 ggacggcgag gagcacgtgt acctggggct gggcgacgag ggcaaggtgt ggctgcgcca
     7681 gaagcacgcg ggcatgaagc cgttcaccct ggcctccggc aactacgtgc cggacgcgga
     7741 aggcaacgtg gacctcgagg tgttcatccg cgaccgcctg ttctacgcgc aggtcaacgg
     7801 ccagccgctc ttccagaacc tcatcaatac gcggggccag gcgcgggccg gcatgatcgg
     7861 ctgcagcgtc tggcacccgg acctcggcgc ggcgcgggcg cgggccctgg acttcgagct
     7921 ggagccgttc cgcccgagcg cggtcacgtg agatcccagc cagggccact cgctcgacct
     7981 ggcctactgg ctccgcggcc aggcgtaccg cttcagccac ctcgccgcgc cctggatggt
     8041 cgtctcgacg cacgggctgc agttgcagcc ccggtggaac gaccggctgt tttcctcctt
     8101 cgcttccatc taccggatga aattcatccc cgaggtcagg ctggacagct tcgaggcgct
     8161 cggcaccctg tcgcccaggg cgctggccga agaggcgaag agcatcaagg ccgacgggat
     8221 tctgctgaac atgggggcca tcatgcgcgg aatgatcctc tcgcagctga cttcgtggct
     8281 tcaggacgcc agccaggtcc tggcggccag cgggctgacc atgctcgtgc aactgccgcc
     8341 ggtgctggag cagccctcca ccctgccgag cgtgatccag gccctccccg aggcgcaggt
     8401 cgtcacgacg gaagacgcca ccgtggcagt cgcggtggag gcctccgcga cgaacccgcc
     8461 cgtggtgctg aaggaggaaa ccgcccccct gccctaccgc gacctcgagc tgacgctcta
     8521 ctacgaactc tccggcctga cccagcccga cgagacctgg agcacggaga tgcacgcgga
     8581 atggctgcgc caggagggcc gggccgcgtt ccgcgtcggc gacttccaga aggcgcggga
     8641 gatctggacg cactggaagg agatcgagcc ctccaacgag gagcccgtct cgctgatcgg
     8701 cgacgtcttc ctgcgcgagg gcgacatcgg gaaggcgatg gatttctacc ggcagagcct
     8761 cgacctcaac ccgggccaga tcggcctggt gatccgcatg tcccgcatcc tggacgtcaa
     8821 cgcgaagaag cccgaggagg cgatgcagct gctgaaccgg tacgcccgga tcttccccaa
     8881 ccatcccgac atcgccctgg cgcaggccga gtggctgatc cggcagcggc gcgacgagga
     8941 ggccaccgcg
//
LOCUS       DAOOTK010000122         8846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5020_length_8846_cov_4.306222, whole genome shotgun sequence.
ACCESSION   DAOOTK010000122 DAOOTK010000000
VERSION     DAOOTK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5020_length_8846_cov_4.306222"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /gene="metG"
                     /locus_tag="PK634_11750"
     CDS             <1..1619
                     /gene="metG"
                     /locus_tag="PK634_11750"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOW98451.1"
                     /translation="YDGWYCVPDERFFMEKDLADGKCPECGRPVEKLTEANYFFRMSK
                     YQEWLVQYIEEHPAFIQPESRRNETLGFLRKPLTDLCISRPKSRMGWGIDLPFDPEYV
                     TYVWFDALVNYVSAVGYLEDSALFAKWWPASFHLVGKDILTTHTVYWPTMLKAMDLPM
                     PETVFAHGWWLSGEDKMSKSRGNVVRPMDMIERVGVDAFRYFLIAEMVMGQDASFTED
                     AFTRRYNADLANDLGNLLNRVLAMIGKYCGGAMPEPSAAALEEPDGKALWDKVQGAVR
                     GMEAALEQMKLNEGIGQVIAAVREVNRFLERRQPWTLAKSEDPVPLRNCLYAASEALR
                     VLGGLLYPVMPGKMAELRKALGMARPKPDIRRFGAWGVLKPGVPVRAPGALFPRLVDE
                     KEKGAEQQTELIPTAPAAAPAAPAPSVPPPAAEKPPAAEGLIEYGDFSKVQLRTAKIL
                     QAFKIQGATKLLRLEVLMGEERRQIVAGIALHYEPEQLIGKTIVMVANLKPAVIRGVE
                     SQGMLLAATKGERLKLVTVDGDQASGAVVK"
     gene            complement(1632..2720)
                     /locus_tag="PK634_11755"
     CDS             complement(1632..2720)
                     /locus_tag="PK634_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOW98452.1"
                     /translation="MTIDPRIANLARILVRYSVHARPRELIALDATPEATPLVLAAYE
                     ELLRAGAYPIVRLNPAETAEIFYRHARPHHLDRPHAIQRAMAERLDGSIHIESSPNTR
                     ALSRVDPRKQARVSRATLPLREKMLHKKWSLTLFPTQAYAQDADMSLADFEDFVYAAT
                     FADRSDPIAAWEQLSRRQEKLVRRLRGARAVRIVGPDTDLSLSVQGRVFINSDGHHNM
                     PSGEVFTGPVEDSAEGHIRFDYPVCHAGREVDDVRLVFRKGLVVEASATKNEKFLRAM
                     LDMDPGARRLGELGIGTNYGIDRFIKNILFDEKIGGTIHLALGRSYEETGGRNRSALH
                     WDMIKDLRRGGALLVDGEVFQKDGRFRP"
     gene            2917..3399
                     /locus_tag="PK634_11760"
     CDS             2917..3399
                     /locus_tag="PK634_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOW98453.1"
                     /translation="MENGDIGRSDETLEKGVFVNTYTHYLDSKRRLTIPAVWRELVGV
                     PRRLFVLQGVNDQCLCVYPARELIRRMERLRNLSIADEQGRQFARALASRAELVPWDS
                     QGRVRIKDELLDFAALSTEVMMAGTFDGFELWSPEKWKEKTGVLDQGRMGEAARYVGF
                     "
     gene            3543..3875
                     /locus_tag="PK634_11765"
     CDS             3543..3875
                     /locus_tag="PK634_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98454.1"
                     /translation="MSAPAVESVEAPVEEPAPVSLHVGSVENRHTILAARPPEKGKIM
                     SPIAIAKFLRISGSVYDGRDEGRWFALPSRKKSVPFQFMRQVAISVDDRRAWMSSERI
                     GSRRSDGL"
     gene            3888..4811
                     /gene="rsmH"
                     /locus_tag="PK634_11770"
     CDS             3888..4811
                     /gene="rsmH"
                     /locus_tag="PK634_11770"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOW98455.1"
                     /translation="MHVPVLLRETLEALGVRAGGTYIDGTVGNGGHAAEMLSRAGPGG
                     RLLGIDQDAETLPRAAERLSRAGGWYRLEQGDYADMRALALRNGIEQADGVLLDLGMS
                     SEQVDDPARGFSFQQDGPLDMRMDRMQERTAATIVNEWTEKELADLLWALGEEPMARR
                     IARRIVEARDREPIRHTGKLAEIVARAKGGRRGRIHPATQTFQALRMAVNHELESLDR
                     ALAAAPGLLALGGRLAVISFHRLEDRRVKAAMALHVGRWESLAAGGRRWVGERPVLRW
                     ITRKPVTCSPEERGANPRARSAKLRVAERIE"
     gene            4815..5198
                     /locus_tag="PK634_11775"
     CDS             4815..5198
                     /locus_tag="PK634_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98456.1"
                     /translation="MRRRKRQNRKTIQEGSPFPMSLALILVLVTGLAFGYLWLRDQCD
                     TTGRRLQELESRRGDLQRQILREESKWSNLKSAKNLEQLMAQFHLAMVWPGEQYTKRL
                     SRPELEPEAAAEMQYAQRELATRHD"
     gene            5191..6939
                     /locus_tag="PK634_11780"
     CDS             5191..6939
                     /locus_tag="PK634_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOW98457.1"
                     /translation="MTDWKYRIRMDLVAGLLLIALMALAARIVQLHVGPNDSLRRRID
                     QTRRYEDRLPEPRGRILDRHGNILAIDLAMRNVIADPSEVLASGNPRFIGLQLARVLQ
                     LDPAMVMARLNRPNRRFEYIQQRVPDETADLIRRMQLRGVSFDEVSARHYPQGPLACH
                     VIGFANLENVGSAGIEQRFHTYLRGQPGLRVSEKDGRRREIFDRRVLDIPPQRGADIY
                     LTIDQNLQYMVEKALESAIAEHQAKGAWAVVQRVRTGEILAMASLPGFDLNEYRSADD
                     LQKLNRAIGYVYEPGSTFKVATIAAALNEHRVTPDQVFDCENGCWFFKGRPLRDYHPN
                     GLLSVADVLKKSSNIGAAKITLTMEEKTVERYLRAFGYGAPTGVELPGEEGGILADRS
                     QWTSLSLSRIAMGHEVGVTALQMLNMLCAVANDGMRVRPTIIRRLVDAEGHTLMESKP
                     EFVAQPIRSETSALMRKLLARVTEEGGTGTRAAVEGYTVAGKTGTAEKAINGSYSSRA
                     NVSSFMGFLPAEDPDRIAIMVVVDEPQGAFRTGGVVAGPVFREIARQAVRYLDIPPDV
                     PVEAGVENKPEPWRSL"
     gene            6950..8446
                     /locus_tag="PK634_11785"
     CDS             6950..8446
                     /locus_tag="PK634_11785"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOW98458.1"
                     /translation="MILGQLCVETMRLEYLTRVIQPVTLRGATDCEIEGLAYDSRQVR
                     PGFLFVALPGQRRDGRDFVAEAIDRGAVAVMSEKNELPRRAVTCIRVEDARRAMAEVA
                     CALYGEPSRDLFMVGVTGTNGKSTVSFMVRDLLRAAGLAPGLIGTIRYEIGERSIPAS
                     RTTPEAPDLQAMLDRMRRAGCRSAVMEVSSHGLDQKRVWGTDFDVGIFTNLTQDHLDY
                     HRTMDRYYAAKSLLFRGLGQMEKSARAVLNMDDPWGLQLANTGGFNAELLTFGMHPGA
                     MVRAENVELSDAGSTFDVATPWGGARVSLRLLGRFNVSNALAALAAGGAAGVPPAQMA
                     EVLGAMRAVPGRLEPIPAARPFRVYVDYAHTPDALTNVLRTLREFTAGRVIVVFGCGG
                     DRDRAKRPMMGAAAASLADYSVVTSDNPRSELPADIIAEIVPGFGEASNFEIEENREK
                     AVFKALRMARAGDTVLIAGKGHETTQEIGNTISPFDDREVARRLLENL"
     gene            8473..8655
                     /locus_tag="PK634_11790"
     CDS             8473..8655
                     /locus_tag="PK634_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98459.1"
                     /translation="MEEKHIMVKCCRCRRVRLQDRWADEAEEPAGSPLYSHSYCPACS
                     EQVHAELRSCSVVAAC"
BASE COUNT         1454 a         2832 c         3179 g         1381 t
ORIGIN      
        1 agtacgacgg ctggtactgc gtgccggacg agcggttctt catggagaag gacctggcgg
       61 acggcaagtg ccccgagtgc gggcggccgg tcgagaagct cacggaggcg aattacttct
      121 tccggatgag caagtaccag gagtggctcg ttcaatacat cgaagagcac ccggccttca
      181 tccagcccga gtcgcggcgc aacgagacgc tcggcttcct gcgcaagccg ctgaccgacc
      241 tgtgcatctc gcgcccgaaa agccggatgg ggtggggcat cgaccttccg ttcgaccccg
      301 agtacgtcac ctatgtctgg ttcgacgcgc tggtcaacta cgtcagcgcg gtcggctacc
      361 tggaggattc cgcgctcttc gcgaaatggt ggcccgccag cttccacctg gtcggcaagg
      421 acatcctcac cacgcacacc gtgtactggc ccacgatgct caaggccatg gacctcccga
      481 tgccggagac cgtgttcgcg cacgggtggt ggctgtccgg cgaagacaag atgagcaagt
      541 cgcgcggcaa cgtggtccgc ccgatggata tgatcgagcg ggtgggcgtg gacgccttcc
      601 gctatttcct gatcgcggag atggtgatgg ggcaggatgc gtcgttcacc gaggatgcct
      661 tcacgcgccg gtacaacgcc gatctcgcga acgatctcgg caacctgctc aaccgcgtgc
      721 tggcgatgat cggcaagtat tgcggcggcg cgatgcccga gccgtccgcg gcggcgctgg
      781 aggagcccga cggcaaggcg ttgtgggaca aagtccaggg cgcggtgcgg ggcatggagg
      841 ccgcgctgga gcagatgaag ctcaacgagg ggatcgggca ggtgatcgcc gccgtccgcg
      901 aggtcaaccg gttcctggag cggcgccagc cgtggacgct ggcgaagtcc gaggatcccg
      961 tgccgctgcg caactgcctg tacgccgcgt ccgaggcgct gcgcgtgctg ggcgggctgc
     1021 tctatcccgt catgcccggc aagatggcgg aactgcgcaa ggccctcggg atggcccgcc
     1081 cgaagccgga catccgacgc ttcggcgcat ggggcgtttt gaaaccgggc gtgcccgtgc
     1141 gcgcgccggg cgcgctgttc ccgcgcctcg tcgatgaaaa ggaaaagggc gcggagcagc
     1201 agactgaatt gatccccacc gcgccggccg cggcgcccgc ggcccccgcg ccgtccgttc
     1261 cgcctcccgc cgcggagaag ccgccggcgg ccgaggggct gatcgagtac ggcgacttct
     1321 cgaaggtcca gcttcgcacc gcgaagattc tccaggcctt caagatccag ggcgccacga
     1381 agctgttacg tctcgaggtg ctgatgggcg aggagcggcg gcagatcgtc gcgggcatcg
     1441 cgctgcatta cgagccggaa cagttgatcg gtaagaccat cgtcatggtg gccaacctga
     1501 agcccgccgt catccgcggc gtggagtcgc agggcatgct gttggccgcg accaagggcg
     1561 aacggttgaa gctggtcacc gtcgacggcg accaggccag cggcgccgtg gtgaagtagc
     1621 cgcgacgcag atcaaggcct gaaccgcccg tccttctgga acacttcccc gtcgacaagg
     1681 agggccccgc cgcggcggag gtctttaatc atatcccagt gcaaggccga gcggttacga
     1741 ccgccggttt cttcgtagga gcggcccagc gcgaggtgga tcgtgccgcc gatcttctcg
     1801 tcgaagagga tgttcttgat gaagcggtcg atgccgtagt tggtgccgat gcccagctcc
     1861 ccgagccggc gcgcgccggg atccatgtcc agcattgcgc gcaggaattt ttcgttcttc
     1921 gtcgccgagg cttcgacgac aagccccttg cggaacacga ggcggacatc gtccacctcg
     1981 cggcccgcgt ggcagacggg atagtcgaag cggatatggc cctcggccga atcctcgacc
     2041 ggcccggtga acacctcgcc cgacggcatg ttgtggtggc cgtccgagtt gatgaacacg
     2101 cggccctgca cggacagcga cagatcggtg tcgggcccga cgatgcggac ggcgcgcgca
     2161 ccgcgcagcc ggcgcacgag cttttcctgg cggcgcgaga gttgttccca cgccgcgatc
     2221 ggatcgctac ggtccgcgaa cgtggcggcg tagacgaagt cctcgaagtc ggcgaggctc
     2281 atgtccgcgt cctgcgcata ggcctgggtc gggaagaggg tgaggctcca tttcttgtgg
     2341 agcatctttt cgcgcagggg cagggtggcg cgggagacgc gcgcctgctt gcggggatcc
     2401 acgcgcgaga gggcgcgcgt gttggggctg gactcgatgt gaatggagcc gtcgaggcgt
     2461 tcggccatgg cgcgctggat ggcatggggc cggtcgaggt gatgggggcg cgcgtgccgg
     2521 taaaagatct cggcggtttc ggccgggttc aggcgcacga tggggtaggc gccggcgcgc
     2581 aggagttcct cgtacgcggc caggacaagg ggcgtggcct cgggggtggc gtcgagagcg
     2641 atcagttcgc gcgggcgggc gtggacgctg tagcggacca ggatcctggc caggtttgcg
     2701 atgcgtggat ctatggtcat gggcgttctc ctgttccaac tgaacacggc atggttagcc
     2761 atggatgagg aaatagtaca ataactttct tttcctccgc tgttcagggc tgaaagcagc
     2821 gtcaaatcaa cgggttccac aaccggttaa attgttaaaa aagtgttgac ggaaggtggg
     2881 gcaaagtggt ttaaagtgtg cctggaggca actgctgtgg agaacgggga catagggcgg
     2941 tctgatgaga cgctcgagaa gggcgttttc gtcaatacct acacccatta cctggactcc
     3001 aagaggcggt tgaccatccc tgcagtttgg cgcgagttgg tgggcgtacc gaggcggctg
     3061 tttgttctcc aaggtgtcaa cgaccagtgc ctgtgcgtct accccgcccg cgagttgatt
     3121 cgccgcatgg aaagactccg caatctgtcc attgccgacg agcaaggcag acaattcgcc
     3181 cgggcgctcg cctctcgcgc tgaactggtt ccctgggatt cccaggggcg cgtccgaatc
     3241 aaggacgagc tgctggattt cgccgcgctg tccaccgagg tgatgatggc cggcacgttc
     3301 gacggatttg aattgtggag ccctgaaaaa tggaaagaga aaaccggagt gctcgaccag
     3361 ggccggatgg gcgaggccgc ccgctacgtc ggcttctagg gaacatcggc gtccgacccg
     3421 cgggggacca tcgtccggcg gcgtggtggg ccgatcccgc cgcgccggcg tcgcgcgagc
     3481 gccaggcgga attctgggaa gagccggcga ccacctcggc ggccgtcgca gtgatcgagc
     3541 cggtgagcgc gccggcggtc gagtcggttg aggcgccggt agaggagccg gcgccggttt
     3601 cgctgcacgt cggctcggtg gagaaccgcc atacgatcct ggccgcccgc ccgccggaga
     3661 aagggaagat catgagcccg atagccattg ccaagtttct gcgcatcagt ggttcggtgt
     3721 acgacggccg cgacgagggc cgatggtttg ccctgccgtc gcgcaagaag agcgtgccgt
     3781 tccagttcat gcgccaggtg gcgatctccg tcgacgaccg gcgcgcctgg atgtcctccg
     3841 agcgcatcgg gtcgcgccgc tccgacggcc tgtaagcgga gggcggcatg cacgtgcccg
     3901 tcctgctgcg ggagaccctc gaggcgctgg gggtccgggc gggcggtacg tacatcgacg
     3961 ggacggtagg gaacggcggg catgcggccg agatgttgag ccgggccgga ccgggcggtc
     4021 gactgctggg gatcgaccag gacgccgaga cgctgccgag ggcggcggag cggctctcgc
     4081 gggccggggg ctggtaccgg ctggaacagg gggattacgc ggatatgcgg gcgctggcgc
     4141 tccggaacgg tatcgagcag gcggacgggg ttctcctgga cctcgggatg tcgtcggaac
     4201 aggtggacga tcccgcccgc ggattcagct tccagcagga cgggccgctg gacatgcgca
     4261 tggaccgcat gcaggagcgg acggcggcga cgatcgtgaa cgagtggacc gagaaggaac
     4321 tggcggacct gctctgggcg ctgggcgagg aaccgatggc ccggcggatc gcgcgccgga
     4381 tcgtggaagc ccgggaccgg gagccgatcc ggcataccgg aaagctggcg gagatcgtgg
     4441 cgcgggccaa gggcggccgg cggggtcgga tccatccggc gacgcagacg ttccaggcgt
     4501 tgcggatggc ggtgaaccat gagctggaga gcctggaccg ggccctggcc gccgcgccgg
     4561 gcctgctggc gctgggcggc cggctggcgg tgatctcgtt ccaccggctg gaagaccggc
     4621 gggtcaaggc ggccatggcg ctccacgtgg ggcgctggga gtcgctggcg gccggcgggc
     4681 ggcgctgggt gggggagcgg ccggtcctgc gctggatcac gcgcaagccc gtgacgtgct
     4741 ctccggagga gcgcggggcc aatccccggg cgcgttcggc caagcttcgg gtggcggaaa
     4801 ggatcgagta ggacatgcgg cggcgaaaaa gacagaaccg gaagacgatc caggaaggat
     4861 cgccgttccc gatgtccctg gccctcatcc tcgtgctggt gacggggctg gccttcggct
     4921 acctctggct gcgggaccag tgcgacacga cgggccggcg gctgcaggag ctggagtcgc
     4981 ggcgcggcga cctccagcgg cagatccttc gcgaggaatc caagtggtcg aacctgaaga
     5041 gcgcaaagaa tctcgagcaa ctcatggccc agtttcacct ggccatggtc tggcccggcg
     5101 agcaatacac caagcgcttg tcccggccgg agctggagcc cgaagccgcg gcggagatgc
     5161 agtacgcgca acgggaactg gcgacgcgcc atgactgatt ggaagtacag gatccgtatg
     5221 gacctggtgg cgggcctgct gctcatcgcg ctgatggccc tcgccgcgcg catcgtgcag
     5281 ctgcacgtgg gccccaacga cagcctgcgc cgccgcatcg accagacccg ccgctacgag
     5341 gaccgcctgc ccgagccgcg cggccgcatc ctggaccggc acggcaacat cctggcgatc
     5401 gacctggcga tgaggaacgt catcgccgac ccgagcgagg tcctcgcgag cgggaacccc
     5461 cgcttcatcg ggctgcagct ggcgcgcgtg cttcagctgg accccgccat ggtcatggcc
     5521 cgcctcaacc ggccgaaccg gcggttcgag tacatccagc agcgcgtgcc cgacgagacg
     5581 gccgacctga tccgccggat gcagctgcgg ggcgtgagct tcgacgaggt cagcgcgcgg
     5641 cactatcccc agggcccgct cgcctgccat gtcatcggtt tcgccaacct ggagaacgtc
     5701 ggcagcgccg ggatcgagca gcgcttccac acctacctcc gcgggcagcc gggcctgcgg
     5761 gtgagcgaaa aggacggacg ccgccgcgag atcttcgacc ggcgcgtcct cgacatcccg
     5821 ccccagcggg gggcggacat ttacctgacc atcgaccaga acctgcagta catggtggag
     5881 aaggccctcg agtccgccat cgccgagcac caggcgaagg gcgcctgggc ggtcgtccag
     5941 cgcgtgcgga cgggcgagat tctcgccatg gcttcgctcc cggggttcga cctcaacgag
     6001 taccgttccg cggacgacct ccagaagctg aaccgggcca tcggctacgt gtacgagccg
     6061 ggctcgacgt tcaaggtggc cacgatcgcc gccgcgctga acgagcaccg cgtgacgccg
     6121 gaccaggtct tcgactgcga gaacgggtgc tggttcttca aggggcggcc gctgcgcgac
     6181 tatcatccga acggcctgct ctccgtcgcg gatgtcctga agaagtccag caatatcggg
     6241 gccgccaaga tcacgctgac gatggaggag aagaccgtcg agcgctacct gcgggcgttc
     6301 ggctacggcg ccccgacggg cgtcgagctg cccggcgagg aaggcgggat cctggccgac
     6361 cgctcgcaat ggacctcgtt gagcctgtcc cgcatcgcga tgggccacga ggtcggcgtc
     6421 accgcgctcc agatgctgaa catgctgtgc gccgtggcca acgacggcat gcgcgtccgc
     6481 ccgaccatca tccggcgcct ggtcgacgcg gaggggcaca cgctcatgga gtcgaagccg
     6541 gagttcgtcg cgcagccgat ccgctccgag acgtcggccc tgatgcggaa gctgctggcc
     6601 cgcgtcacgg aggaaggcgg cacgggcacg cgcgccgccg tcgagggcta taccgtcgcg
     6661 ggcaagacgg gcaccgcgga gaaagccatc aacggcagct attccagccg ggccaatgtt
     6721 tcgtcgttca tgggattcct gcccgcggag gatcccgatc gcatcgcgat catggtggtg
     6781 gtggacgagc cgcagggggc gttccgcacg ggcggcgtcg tggccggccc cgtgttccgg
     6841 gagatcgccc gccaggccgt ccgctatctg gacatcccgc cggacgtccc ggtcgaggcg
     6901 ggcgtggaga acaagccgga gccgtggcgg agcctgtgag gagaccgctg tgatcctcgg
     6961 ccagctttgc gtggagacca tgagactgga atacctgacg cgcgtcattc agccggtgac
     7021 cctccgcgga gccaccgact gcgagatcga gggcctcgcc tacgattccc gccaggtgcg
     7081 gccgggcttc ttgttcgtgg cgctgcccgg ccagcggagg gacggccggg atttcgtggc
     7141 cgaggccatc gaccgcggcg ccgtcgccgt gatgtccgaa aagaacgaac tcccgcgccg
     7201 cgccgtgacc tgtatccgcg tggaggacgc ccgccgggcc atggcggagg tcgcctgcgc
     7261 cctctacggc gaaccctccc gcgatctgtt catggtcggg gtgacgggca ccaacggcaa
     7321 gtccaccgtc agcttcatgg tgcgcgacct gcttcgcgcc gccgggctcg cgcccggcct
     7381 gatcggaacg atccgctacg agatcggcga gcgctctata cccgccagcc gcaccacgcc
     7441 cgaggcgccg gacctgcagg ccatgctgga ccgcatgcga cgcgccggct gccgcagcgc
     7501 ggtgatggaa gtctcgtccc acggcctcga ccagaaacgc gtctggggca cggatttcga
     7561 cgtgggcatc ttcaccaatt tgacgcagga ccacctggac tatcaccgga ccatggatcg
     7621 ctattacgcg gcgaagtcgc tgctgttccg gggcctcggc cagatggaaa aaagcgcccg
     7681 cgccgtgctg aatatggacg atccctgggg cctccagctg gcgaacacgg gcgggttcaa
     7741 cgcggagctg ctgacgttcg gcatgcatcc cggcgcgatg gtccgggcgg agaacgtgga
     7801 actgtccgac gccggctcga ccttcgacgt cgcgacgccc tggggcgggg cccgcgtgtc
     7861 gctgcgtctc ctcggccgct tcaacgtcag caacgcgctg gccgcgctgg cggccggcgg
     7921 cgcggcgggc gtaccgcccg cgcagatggc cgaggttctc ggcgccatgc gagccgtgcc
     7981 cggccgcctg gagccgatcc cggcggcccg tccgttccgg gtctatgtgg actatgccca
     8041 tacgcccgac gcgttgacca acgtcctgcg gaccctccgg gagttcaccg ccggccgcgt
     8101 catcgtggtc ttcggctgcg gcggcgaccg ggaccgcgcc aagcggccga tgatgggcgc
     8161 cgcggccgcg agcctggcgg attattccgt ggtgacgagc gacaacccgc ggagcgagct
     8221 gcccgcggac atcatcgccg agatcgtgcc gggattcggc gaggcctcca atttcgagat
     8281 cgaggaaaac cgcgaaaagg ccgtcttcaa ggccctccgc atggcgcgcg cgggcgacac
     8341 ggtgctgatc gcgggcaagg gccatgagac cacccaggag atcggaaaca ccatctcccc
     8401 gttcgatgac cgcgaggtgg cgcggcgtct gctggagaac ctgtagaccg ggggggccgg
     8461 gagggaaggg gcatggaaga gaaacatatc atggtgaaat gctgccgatg ccgccgcgtg
     8521 cggcttcagg atcgctgggc cgacgaggcc gaggagcccg ccggatctcc gttgtactcg
     8581 cacagctact gccccgcctg cagcgaacag gttcatgccg agctgcgttc gtgctcggtc
     8641 gtcgccgcgt gctgaccccg ccgctcccgt tgttcccgtc atgcccctct ttccggcaga
     8701 tgtcctggcg tcgtggtccg gcggaacgtg gtccggtccg ccggccgctc ccgcgcgcgg
     8761 cgtctgcgcc gacacccggc gtcttcgcgc cggcgacctg tatgtggcga tccgcgggcc
     8821 gcgcttcgac ggccacgatt tcatcg
//
LOCUS       DAOOTK010000123         8245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5447_length_8245_cov_3.701587, whole genome shotgun sequence.
ACCESSION   DAOOTK010000123 DAOOTK010000000
VERSION     DAOOTK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_8245_cov_3.701587"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PK634_11795"
     CDS             complement(<1..622)
                     /locus_tag="PK634_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98460.1"
                     /translation="MRRLLSIVGLMLVLTSGPAVAVTNTLSVYLLLADEPAALAAARE
                     AGRQGGRFGLSARQAGLDAASRIESRQQALRSALAGTGARETGRFRRLVNAVRVDVPA
                     DELDAMWTLPGVVDIRPVRQFERNNATSVPFVGGLNAWTSAALRVDGSGVRIGIIDSG
                     IDYTHAMFGGAGTTNAFATNDSTVIEPGTFPTAKVAGGWDFCGDDYD"
     gene            736..1314
                     /gene="pyrE"
                     /locus_tag="PK634_11800"
     CDS             736..1314
                     /gene="pyrE"
                     /locus_tag="PK634_11800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOW98461.1"
                     /translation="MQPEQVLAEFQDTGALLHGHFELRSGLHSDQYFQCALVLQHPRL
                     AERLCSALAGMVRERLGAAYRPGAVISPALGGIPVGHELGRAFNVRAIFAEKQDGRLV
                     MRRAFEVRPGEHFLVAEDVVTRGGRVHEVMNIVRERGGIVDGVAVLVDRSAGHFACER
                     PVIPLLQLAPVTWAPAECPLCRTGQPVQHPGS"
     gene            1459..1884
                     /locus_tag="PK634_11805"
     CDS             1459..1884
                     /locus_tag="PK634_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOW98462.1"
                     /translation="MLKLSKKVEYALMALLHMEGRRAAELATAREIADSCGAPVELLG
                     KVLQALARGGLIESEQGARGGYRLARPADRITLGEVIEVVDGPVHIAACQDDPSRCGQ
                     YPTCNIRQPVVRVQRELQDYMYKLSLAAFRREPAGAATR"
     gene            1948..2232
                     /locus_tag="PK634_11810"
     CDS             1948..2232
                     /locus_tag="PK634_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOW98463.1"
                     /translation="MEVLRHVYDPELQMDIVELGLVYGVELEPDGRVKVNMTLTSPGC
                     PYGPELVAEVKATLMMARGVKGVEVNVVWDPPWGPEKMSEAARLDLGFDV"
     gene            2312..3091
                     /gene="sufC"
                     /locus_tag="PK634_11815"
     CDS             2312..3091
                     /gene="sufC"
                     /locus_tag="PK634_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOW98464.1"
                     /translation="MSECGADFEIKDLCASVEGKPILKGVNLRVNRGETHALMGPNGS
                     GKSTLASVIMGHPGYEVTGGQICFKGRNILDLETEERARLGIFLAFQYPVAIPGVTVA
                     KFLKTAADAMRPGGAKVSSAAFLKELREYMAFLEMDPAFINRFLNDGFSGGEKKRMEI
                     LQMLALKPAFAVMDETDSGLDIDALKIVSKGINRLVGPDFGLLLITHYERMLRYIKPD
                     HLHILMDGRIVHSGGPELVKRLEEKGYDWVRQEFGKEVLAE"
     gene            3088..4515
                     /gene="sufB"
                     /locus_tag="PK634_11820"
     CDS             3088..4515
                     /gene="sufB"
                     /locus_tag="PK634_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOW98465.1"
                     /translation="MSTPETPDVLKDEYKYGFYTDIEADSAPKGLNEDIIRFISRKKG
                     EPDWMLEWRLKAYRGWLKKKEPRWAYLDYPPIDYQDICYYSAPKTGPAGEVDEELRKT
                     FERLGIPLDERKRLEGVAVDAVFDSVSVATTHKDILAEKGILFCPISEAIREHPDLVR
                     KYLGSVVPPADNFFAALNSAVFTDGSFCYIPKGVRCPMELSTYFRINARNTGQFERTL
                     IVADEGSYVSYLEGCTAPLRDENQLHAAVVELIALDDAQIKYATVQNWYAGDKEGRGG
                     IYNFVTKRGHCRGQRSRISWTQVEVGSAITWKYPGCVLEGEHSVGEFYSVALTNHRMQ
                     ADTGTKMIHIGSHTRSTIVSKGISAGESVNSYRGLVQVLPDAVGCRNHSQCDSMLIGD
                     KCKANTFPVVEAENGSAVIEHEATTSRISEDQLFYLKSRGITEEDAVSLVVNGFCKEV
                     FKQLPLEFAVEAIRLLELKLDKSIG"
     gene            4521..5819
                     /gene="sufD"
                     /locus_tag="PK634_11825"
     CDS             4521..5819
                     /gene="sufD"
                     /locus_tag="PK634_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOW98466.1"
                     /translation="MSTSISGNLVAGFDEARFGDLVNRAGSRESRERAFERYRALPLP
                     HGQIEEWRRTDPAWFPFGRLAFDPALGRGKDGAGSGPGDDRFAAVVTIGDDGMSVRDV
                     SGAIRAGQLEIASLADGEPGFDLVQRGFDVGVKSAEPNKMALLSRSFWNVGVRVRVPD
                     GVELERGVLVRSSFSKDGSLVIPRIAVEAGRGSRAVILEELSSPADRTLLVAAAREMW
                     ASENAALQYVALQDLGGQSFYLADDRAHAERNGRVHWIGLNLGARLGKMNFSGEVAGE
                     GAAAELDGLYFASGEQHLDQRTLQIHSAPHTTSNLLYKGAVRDRARSVYQGLIIARPG
                     AVKVDAYQKNNNIVLNEGARADSLPGLEIDTDDLKCSHGSTIGNLDAEQVFYLRTRGM
                     DERDARRTLLKGFFEEIAARVPFEFVRDRIERHVEEQLGR"
     gene            5862..6173
                     /locus_tag="PK634_11830"
     CDS             5862..6173
                     /locus_tag="PK634_11830"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOW98467.1"
                     /translation="MAEWVQLAETREFETADRKYVELSPMKHIGLFQVGGEYFAIEAW
                     CSHARASLVHGEVEGHEVLCPLHGARFDLRTGRHLSPPAVRPVARYDVKVEDGKIFVK
                     V"
     gene            6175..7479
                     /locus_tag="PK634_11835"
     CDS             6175..7479
                     /locus_tag="PK634_11835"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOW98468.1"
                     /translation="MTTPSSAPSTLAALVHCACHEQGGGGPFDPLRLRKDFPILTLTV
                     HKDKTLAYLDNAATTQKPASVVCVLCEYYQACNANVHRSLHYLAEQATLRFEEARRKV
                     QRFIGAPSERSVIFTRGTTEGINLVAQAWGRRNLKPNDEVVLTGMEHHSNLVPWQLVA
                     KETGAKLKFVPVLDDGALDLDAYGRLLKGRVRLAAFTHISNVLGTVNPVKQMTAMAHA
                     AGALVLVDAAQSVPNRPVNVADIDCDFLAFSGHKMLGPTGIGVLYGRLEILERMEPFL
                     AGGEMINRVTLEESTWADLPQKFEAGTPHVSGAIGLGVAVDYLQRVGMETIRAYDEHL
                     ARSMIARLDAIPGVRVFGRAPERGGAVSFEVEGVHPHDLAQFCDKDGIAIRAGHLCAQ
                     PLMQRFGVPAVSRASVYFYNVEEELDRLVQSILKAKAFFHGA"
     gene            7469..7897
                     /locus_tag="PK634_11840"
     CDS             7469..7897
                     /locus_tag="PK634_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOW98469.1"
                     /translation="MELDELYQDILLDHFKHPRNYGPLPEPEVLVDELNPTCGDHIRL
                     TARLENGRVADVRFDGQGCAISLASASMMSELMRGLTPDEARARAADFVAFMRGEKDL
                     DPDTLGDLAALAGVRQFPLRIKCATMSWHALEKALGKLGG"
     gene            8000..>8245
                     /locus_tag="PK634_11845"
     CDS             8000..>8245
                     /locus_tag="PK634_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98470.1"
                     /translation="MKTMQILAVLAVVAAVALPQRAEAINREWSAVLGFLGGYMVANG
                     GLCPPAPCVSRPVYVERPVYVERPVVYCPPPPPPPPVV"
BASE COUNT         1412 a         2638 c         2915 g         1280 t
ORIGIN      
        1 agtcataatc gtccccgcag aagtcccacc cgccggccac cttggccgtc gggaacgtgc
       61 ccggctcgat gaccgtggaa tcgttcgtcg cgaacgcatt ggtcgtgccg gcgccgccga
      121 acatggcgtg cgtgtagtcg ataccggaat cgatgatccc gatgcgcacg cccgatccgt
      181 cgacgcgaag ggccgccgag gtccacgcgt tcagcccgcc gacgaacggc acgctcgtcg
      241 cgttgttccg ctcaaattgc cgcaccggcc ggatatccac cacgccgggc agggtccaca
      301 tcgcatccag ttcgtccgcg ggcacgtcca cgcggacggc gttgaccagg cggcggaacc
      361 ggcccgtttc gcgggctccc gtcccggcca gcgccgagcg cagggcctgc tgccggctct
      421 cgatccggga tgccgcatcg agcccggcct gtcgggccga aaggccgaat cgaccgcctt
      481 gccggcccgc ctcgcgggcc gccgccagcg ccgccggctc gtcggccagc aggagataga
      541 cggataacgt gttcgtgacg gccacggcag gtccggacgt caggaccagc atcaagccca
      601 ctatcgaaag aagtcgtctc atgagagaac atccttgtgc tgaacatgaa gtgtgggagc
      661 atcctacccg ccggttcgcg ccgactcaag gacgggcgtt gacttcgccg cccggcttgt
      721 accatcatcc ggtccatgca accggaacag gtgctggcgg aattccagga cacgggcgcg
      781 ctgctccacg gccatttcga gctgcggtcg ggcctccata gcgaccagta cttccaatgc
      841 gcgctggtcc tccagcatcc gcggctggcg gagcggcttt gttccgcgct ggccggaatg
      901 gtccgcgaac ggctcggcgc ggcctaccgg cccggggccg tgatctcccc cgcgctcggc
      961 ggcatacccg tcggccacga gctgggccgc gccttcaacg tccgggcgat cttcgccgag
     1021 aagcaggacg gacgcctggt catgcgccgc gccttcgagg tgcggccggg cgagcacttc
     1081 ctcgtggccg aggacgtcgt cacccgcggc gggcgcgttc acgaggtcat gaatatcgtg
     1141 cgggaacgcg gcgggatcgt ggacggggtc gcggtcctgg tggaccggag cgccggtcat
     1201 ttcgcctgcg agcggcccgt gattccgctc ctccagttgg cccccgtgac ctgggctccc
     1261 gccgaatgcc cgctatgccg gacgggacag cccgtgcagc atccgggaag ctgacaggcg
     1321 gcgtcgcggg aaatccgccc tccaattgcg tgagttcccg caagcctgat ttgacaggat
     1381 caatagacga tgtagattgc tccagctttc gggcgttttt tttagcccgg aacacggacc
     1441 gaattggtcg gatatgccat gctgaagttg agcaagaaag tcgaatacgc gctgatggcg
     1501 ctgctgcaca tggagggccg gcgggcggcg gaactggcga cggctcgcga gatcgccgac
     1561 tcctgcggcg cgccggtgga actgctgggc aaggtgctcc aggcgctggc gcgcgggggc
     1621 ttgatcgaat cggagcaggg cgcgcgcggc gggtaccgcc tggcgcggcc ggcggaccgg
     1681 atcacgctgg gcgaggtgat cgaggtggtg gacgggccgg tgcacatcgc cgcgtgccag
     1741 gacgacccgt cgcgctgcgg gcagtacccg acctgcaaca tccgccagcc cgtggtccgg
     1801 gttcagcgcg aactgcagga ttacatgtac aagctatcgc tggccgcgtt ccggcgcgaa
     1861 ccggcgggcg ccgcgacccg gtgaaccatg acctcgaatg aatcaaagcc ctcgggatca
     1921 acggagcccc ttcgggagcc ggacctgatg gaggtcctgc gccacgtgta cgatccggaa
     1981 ctgcagatgg acatcgtcga gctgggcctc gtgtacggcg tggaactcga gcccgacggg
     2041 cgggtgaagg tgaacatgac gctgacctcg cccggctgcc cgtacggccc ggagctggtg
     2101 gccgaggtca aggcgacgct gatgatggcc cgcggcgtga agggcgtcga ggtgaacgtg
     2161 gtctgggatc cgccgtgggg gccggagaag atgagcgagg ccgcgaggct ggatctgggg
     2221 tttgatgtgt agatgaaaag gtagggcgcg ctcgatgagc gcgccgcggc ggcttgagcc
     2281 aagccgccct acccgtgagg ggagagggcc aatgagcgaa tgcggagcag acttcgaaat
     2341 caaggacctg tgcgcgagcg ttgagggcaa gccgatcctc aagggcgtga acctgcgggt
     2401 gaaccgcggc gaaacgcacg ccctgatggg cccgaacggc tccggcaaaa gcacgctggc
     2461 cagcgtgatc atgggtcatc ccggctacga agtcaccggc ggccagatct gtttcaaggg
     2521 gcggaacatt ctcgatctgg aaaccgaaga gcgcgcgcgg ctgggcatct tcctggcctt
     2581 ccagtacccc gtggccatcc ccggcgtgac cgtggccaag ttcctgaaaa ccgccgccga
     2641 cgccatgcgg ccgggcggcg cgaaggtcag ctccgcggcc ttcctgaagg agctgcgcga
     2701 gtacatggcc ttcctcgaga tggaccccgc gttcatcaac cggttcctga acgacggttt
     2761 ttccggcggc gagaagaagc gcatggagat cctccagatg ctcgcgctca agcccgcgtt
     2821 cgccgtcatg gacgagacgg attcgggcct ggacattgac gcgctgaaga tcgtgtccaa
     2881 gggcatcaac cgcctggtcg gcccggactt cggcctgctg ctgatcacgc actacgagcg
     2941 gatgctccgg tacatcaagc ccgaccacct gcacatcctc atggacgggc gcatcgtcca
     3001 ctcgggcggc ccggagctgg tcaagcgcct cgaggaaaaa ggctacgact gggtccgtca
     3061 ggagttcggg aaggaggtgc tcgccgaatg agcacgccgg aaacaccgga cgtcctcaag
     3121 gacgagtata agtacggctt ctacaccgac atcgaggccg actccgcgcc aaaggggctg
     3181 aacgaggaca tcatccgttt catctcgcgg aagaaaggcg agccggactg gatgctcgag
     3241 tggcggctga aggcctaccg cggctggctg aagaaaaagg agccgcgctg ggcgtacctg
     3301 gactacccgc cgatcgatta ccaggacatc tgctactact ccgcgccgaa gaccggcccg
     3361 gccggcgagg tggacgaaga gctgcgcaag acgttcgagc ggctcggcat cccgctcgac
     3421 gagcgcaagc ggctcgaggg cgtcgccgtg gacgcggtgt tcgacagcgt ctccgtggcc
     3481 acgacgcaca aggacatcct cgcggagaag ggcatcctat tctgccccat ttccgaggcg
     3541 atccgcgagc atccggacct ggtgcggaaa tacctcggca gcgtcgtgcc gccggcggac
     3601 aacttcttcg ccgcgctcaa ctccgcggtc ttcaccgacg gctcgttctg ctacatcccg
     3661 aagggcgtgc gctgcccgat ggagctctcc acctacttcc ggatcaacgc gcggaacacg
     3721 ggccagttcg agcggaccct gatcgtcgcc gacgagggga gctacgtgtc ctacctcgag
     3781 ggctgcacgg ccccgctgcg cgacgagaac cagctccacg cggccgtggt cgagctgatc
     3841 gcgctggacg acgcgcagat caaatacgcg acggtccaga actggtacgc gggcgacaag
     3901 gaggggcgag gcggcatcta caacttcgtc accaagcgcg gccattgccg cggccagcgg
     3961 tcgcgcatat cgtggacgca ggtggaggtc ggttcggcaa tcacctggaa gtacccgggc
     4021 tgcgtcctgg aaggcgagca ctcggtcggc gagttctact ccgtggcgct gaccaaccac
     4081 cggatgcagg cggacaccgg caccaagatg atccacatcg gcagccacac ccgcagcacg
     4141 atcgtgtcca agggcatctc cgccggcgag tcggtcaaca gttaccgcgg cctggtgcag
     4201 gtgctgcccg acgccgtcgg ctgccggaat cactcgcagt gcgactccat gctgatcggc
     4261 gacaagtgca aggccaacac gttccccgtg gtcgaggcgg agaacggcag cgccgtgatc
     4321 gagcacgagg cgaccacctc gcggatcagc gaggaccagc tcttctacct caagagccgc
     4381 gggatcacgg aggaggacgc cgtctcgctg gtggtcaacg ggttctgcaa ggaagtcttc
     4441 aagcagctgc cgctggagtt cgcggtcgag gcgatccggc tgctggagtt gaaactggac
     4501 aagagcatcg ggtaagggcg atgagcacgt cgatttcagg gaatctggtg gccggcttcg
     4561 acgaggcgcg tttcggcgac ctggtcaacc gggccggctc gcgggagagc cgcgagaggg
     4621 ccttcgaacg ataccgggcg ctgccgcttc cgcacgggca gatcgaggaa tggcggcgga
     4681 ccgacccggc ctggttcccg ttcgggcgcc tggccttcga cccggcgctc gggcggggca
     4741 aggatggagc gggctccggg ccgggggacg accggttcgc cgcggtcgtg acgatcggcg
     4801 acgacgggat gagcgtccgg gacgtgtccg gcgcgatccg ggccggccag ctcgaaatcg
     4861 catccctggc cgacggcgag cctgggtttg acctcgtgca gcgcggtttc gatgtaggcg
     4921 tgaaaagcgc cgagccgaac aagatggcgc tgctgtcgcg gtcattctgg aatgtcgggg
     4981 tccgcgtgcg cgtgccggac ggcgtggaac tggaacgcgg cgtgctggtc cgctcttcgt
     5041 tttccaagga cggctccctc gtgatcccgc gcatcgcagt cgaggccggt cggggcagcc
     5101 gggcggtgat cctggaggaa ctgtcctcgc ccgccgacag gaccctgctc gtcgcggccg
     5161 cccgcgagat gtgggcctcg gagaacgccg cgctccaata cgtggcgctc caggacctgg
     5221 gcgggcagtc gttctacctg gccgacgacc gcgcgcacgc ggagcggaac ggccgggtcc
     5281 actggatcgg gctcaacctt ggcgcgcggc tcggcaagat gaacttcagc ggcgaggtgg
     5341 cgggcgaggg cgcggcggcc gagttggacg ggttgtactt cgcctcgggc gagcagcacc
     5401 tcgaccagcg cacgctgcag attcattccg cgccgcacac gaccagcaac ctgctgtaca
     5461 agggcgcggt gcgcgaccgg gcgcggtcgg tctaccaggg gctcatcatc gcgcggcccg
     5521 gcgcggtcaa ggtggacgcc taccagaaga acaacaacat tgtgctcaac gagggcgcgc
     5581 gggcggactc gctgcccggg ctggagatcg acacggacga cctgaagtgc agccacggct
     5641 cgaccatcgg caaccttgac gcggagcagg tattctactt acgtacgcgg gggatggacg
     5701 agcgggacgc ccggcgcacg ctgctgaagg ggttcttcga ggagatcgcc gcgcgcgtgc
     5761 cgttcgagtt cgtccgggat cgcatcgagc ggcatgtgga ggagcaactt ggaaggtagg
     5821 gcggcgtcga cgaagacgcc cggcctggaa ggggtgaagc catggcggaa tgggtgcaac
     5881 ttgccgaaac cagggaattt gagacggccg accgcaagta cgtcgaactg tcgccgatga
     5941 aacatatcgg gctgttccag gtcggcgggg agtactttgc catcgaggcc tggtgcagcc
     6001 acgcgcgggc gtcgctggtg cacggagagg tggagggcca cgaggtgctc tgcccgctcc
     6061 acggggcgcg gttcgacctg cggaccggcc ggcacttgag ccccccggcc gtgcggccgg
     6121 tggcccggta cgacgtgaag gtggaagatg gaaagatctt cgtgaaggtg tgacatgact
     6181 actccctctt ccgcgccctc cacgctggcc gccctggtgc attgcgcctg ccacgagcag
     6241 ggcggcggag ggccgttcga tccgctgcgg ctgcggaagg atttccccat cctgaccctg
     6301 accgtgcaca aggacaagac gctggcctac ctcgacaacg ccgcgacgac ccagaaaccg
     6361 gcctcggtcg tgtgcgtgct gtgcgagtac taccaggcgt gcaacgcgaa cgtgcaccgc
     6421 tcgctgcact acctggccga gcaggcgacg ctgcggttcg aggaggcgcg ccggaaggtc
     6481 cagcggttca tcggcgcgcc gagcgagcgc tcggtgatct tcacgcgcgg cacgacggag
     6541 ggcatcaacc tggtcgccca ggcctggggc cgccggaacc tgaagcccaa tgacgaggtg
     6601 gtcctgaccg gcatggaaca ccacagcaac ctcgtgccgt ggcagctcgt ggcgaaggag
     6661 acgggcgcga agctgaaatt cgtgcctgtc ctcgacgacg gcgcgctgga cctcgacgcc
     6721 tacgggcggc tgttgaaggg ccgggtgcgc ctggccgcct tcacgcacat ttccaacgtc
     6781 ctgggcacgg tcaacccggt gaagcagatg accgcgatgg cgcacgccgc cggggccctg
     6841 gtcctggtgg acgccgcgca gagcgtgccg aacaggccgg tgaacgtggc ggacatcgat
     6901 tgcgatttcc tggcgttttc cgggcataaa atgctcgggc cgacggggat cggcgtgctg
     6961 tacggccggc tcgaaatcct cgagcgcatg gagccgttcc tcgcgggcgg cgagatgatc
     7021 aaccgggtga cgctggagga atccacctgg gcggacctgc cccagaagtt cgaggcgggc
     7081 acgccgcacg tcagcggcgc gatcggcctg ggcgtggcgg tggactacct gcaacgggtc
     7141 ggcatggaaa ccatccgcgc ctacgacgaa cacctggcgc ggtcgatgat cgcgcggctg
     7201 gacgccatcc ccggcgtgcg ggtattcgga cgcgcgcccg agcggggcgg ggcggtgtcg
     7261 ttcgaggtcg agggcgtgca cccccatgac ctggcgcagt tctgcgacaa ggacggcata
     7321 gcgatccggg ccgggcacct ctgcgcccag ccgctgatgc agcggttcgg cgtgccggcg
     7381 gtgagccggg cgagcgtgta cttctacaat gtcgaggagg agttggaccg gctggtccag
     7441 tccatcctga aggccaaggc gttcttccat ggagcttgac gagctatacc aggacatcct
     7501 cctggaccat ttcaagcatc cccggaacta cgggccgctg ccggaacccg aggtgctggt
     7561 tgacgagctc aacccgacct gcggcgacca catccggctg accgcccggc tggagaacgg
     7621 gcgggtcgcg gacgtgcggt tcgacggcca gggctgcgcc atcagcctgg cgtccgcgtc
     7681 catgatgagc gagctgatgc gcggcctgac gccggacgag gcccgggcgc gcgcggcgga
     7741 cttcgtggcc ttcatgcggg gtgaaaagga cctcgacccg gatacgctgg gcgacctggc
     7801 cgccctggcc ggggtacgcc agttccccct gcgcatcaag tgcgccacca tgagctggca
     7861 tgccctggag aaggcgctgg ggaagctggg ggggtgaacg tcctgttggg gcgtcgctgg
     7921 ccgacgccct tgcgcttttt tatgaataat tcgcatcccc gtccgtctca ctggttggag
     7981 ccaaccatag gaggcggcca tgaagacgat gcagatttta gcggtgctgg cggtcgtggc
     8041 ggcggtggcg ctgccgcaga gggcggaagc gatcaacagg gagtggtcgg ccgtcctggg
     8101 attcctgggc gggtacatgg tggccaacgg cgggttgtgt cccccggcgc catgcgtctc
     8161 gcggccggtc tatgtcgaac ggccggtcta cgtcgagcgg cccgtcgtgt attgtccgcc
     8221 ccccccccct ccgcccccgg tcgtg
//
LOCUS       DAOOTK010000124         8235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5450_length_8235_cov_5.658068, whole genome shotgun sequence.
ACCESSION   DAOOTK010000124 DAOOTK010000000
VERSION     DAOOTK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_8235_cov_5.658068"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="PK634_11850"
     CDS             <1..1693
                     /locus_tag="PK634_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98471.1"
                     /translation="GWAALAVPAAPTGVKASDGTHEDRVEIQWNAVGGATLYQVWRND
                     SNSLNGAASIKNVAGHLNSANDYDATPGQGYYYWVRAENQGGWGPYSGSDSGHVAVAA
                     APPEAPDTLWASDGKYTNKVYLMMQIPPDTTGFDIWRAYQDNVDAAAKIVENHPSGEY
                     SDFDVTPGLYYFYWVQARNADGVSGFSPGDSGYASNAVHAPAKPPCISASQGTYLDRV
                     TIEFCPVEYRTAYHYEIWRNAVDNPAGATCFAILDPTESAATDSNLEPGTTNYYFVTA
                     TNQYGRGPFSDSAKGYTMPGTPAPTNLVASDGTHVGSVLVSWNGVGTTYASVWRHTNN
                     TSAAATCIAPNQNQPYHIDYSATPGQVYYYWAKAFSGSVTSGFSNVDTGYAGADNPPP
                     APGNVQATRGVYNNYIGVTWDKVLDADWYEVWRNTSEDTATATRRDGHTTNNVSWTDP
                     APSWLIGYYYWVRSVNENGTSAWSSAACGYMAGAMSVPEMRVATRLGNIWVMAMTNLT
                     VGLTNYIQSTTNLMPGSQWIQRGAITPSRDRSFWIGLMPGERRFYRIVVNPPDGP"
     gene            1776..4430
                     /locus_tag="PK634_11855"
     CDS             1776..4430
                     /locus_tag="PK634_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98472.1"
                     /translation="MNIHPARLPLLALVLSCCGLASAQVPSLLNYQGRLVNGTNLFNG
                     VISPIFRLYVLPAGGFQIYIEQDLNVPVADGFYSTLLGDNFVGTPLAEALTNNPVYLE
                     VEIGGVRLAPRERVISGGRPLGSLAYRGGDAFVVVSVTNSDTVNAQNLLAAYTVATNR
                     RPHGLPLSAVNRAVVLVPPGRYDLGTGQLTLSCDFVDLVGLSSAREDQHIRGESDGDN
                     TGVLRQTADDARIENLFVECTRYSGGTDADSADPAAYFPDSGRSNTVVRNCRFKAVGE
                     GASWSMRLGVDYAGAYENCAGEAYAFGGGQFGRASGLFVECDGGDFSFGSGFSAQASG
                     RFRRCTGGAGSFGAFFGTASGTFEDCTGGAGSFGGVNASASGTFIRCAGEENSFGALG
                     GSAGGFFLHCWMKGTNWTGGQLSGRMEGCRWEAPVITALSGRVFRSVVEGVFRDDSHF
                     EIGEASVTVKPCRDEELNGRNLLAAYALATNLWPHNRARSASNRVVLLVSPGVYNLGS
                     EALQLTDEYVDLVGVSSARDDQRLAGSADVDYFGVILQSASDVRIENLFVECTRSNGL
                     AVGDADDPAAYFPESGLSNTVIRNCRFKANDTNAWSMRCGIEYAGTYEDCAGGKHSFG
                     GKGGQAAGIFRNCAGGDYSFGFSSGGEASGMFMDCTGGAATFEGMADARGTFDRCTGG
                     SYAFGGGGHATGTYRNCRSDDSAFGGGGGTAGGTFSHCIGGNNCFGSRGGAASGFFLN
                     CVAGDDSFGSHLGCYASGTFINCHAGAMSFGMDGICSGTFIGCRGGLRSFGGNNDEIT
                     GNLYNCWMDSDTWTGNFGGRMEGCRWGAGIMVSSGAVIYASTFAGAVTNFGPGSCQIA
                     HVRCRALDLGPMVNAIATPYNVVDADVE"
     gene            complement(4441..6591)
                     /gene="glgP"
                     /locus_tag="PK634_11860"
     CDS             complement(4441..6591)
                     /gene="glgP"
                     /locus_tag="PK634_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOW98473.1"
                     /translation="MTFEPTSMPDIRMPEEFSRLRELAYNLWWSWTPRARAMFSNISQ
                     PLWALYRNPVQLLIDIEPHHWQTLLSDGGSFMSEYRRVIREFDRYMDPEADTPFKKAY
                     PDYKGGPVAYFSTEFGLHESLGIYCGGLGVLSGDHAKAASDLGLPFIGIGLLYRHGYF
                     RQNIDNDGRQHHLFNESDFYRMPLRPVHTASGRNLLIGVEFPGREVKARVWQLMIGRV
                     KLYLLDTDITENDAADRPITSQLYVRGREMRFCQELLLGVGGARILREMKIKPAVWHL
                     NEGHSAFLLFERLRELMKAGRTWDDAMSTIRRDTMFTTHTPVPAGNEAFEMDLVEKYL
                     GPHAKGMGVAMDRLLELGKAYPGGGGQPFNLTALAIRMSSYSNGVSQLHGDVSNDMWR
                     HLFPQAPHDSKPIKAITNGVHVATWLGHDIRELVEESLGHGWQDRCCDPEFWQGVKKI
                     RDDDLWAAHIHQKERLIRLFRHRLVEQFARHGNSPDEMREVERMLDPKVLLIGFARRF
                     ATYKRAALIFRDYNRLRAILTNPERPVHILFSGKAHPADQPGQDLVRHIYNIAFHSDL
                     RGHIYFVEDYDIRIARHMVQGVEIWLNTPRRPREASGTSGMKAGMNGALNVSISDGWW
                     PEAANGRNGWTINAGRYFDNEDMQDYEDSTSFYHILENEIVPAFYDHRQKGVPANWVA
                     MMKEAMATIIPEFSAARMLQDYTRDAYMPLAGRK"
     gene            6775..7302
                     /locus_tag="PK634_11865"
     CDS             6775..7302
                     /locus_tag="PK634_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98474.1"
                     /translation="MENHIVQRIVASVIAGLCLAVSAPAVMLEEGTQEIQVNGSFDFE
                     GLGGSVLDLGLGWGYFVRDYVEAGLLGEVTLSDPGDSYSIGGFGEYHFDIESIVVPYM
                     GAGLSLLMTDIQNGDDTTALAVSGLAGAKIYLVENVALDIRAVLSIASDDVYIADDEL
                     DNWDARFEMGLRFYY"
     gene            7351..7773
                     /locus_tag="PK634_11870"
     CDS             7351..7773
                     /locus_tag="PK634_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOW98475.1"
                     /translation="MTAARWEHFEHQADVGLRGRGGTPDEAFAQIALALTAVVTDPDR
                     VRAERPVELRCSASDVESLLVDWLNALVYEMAARRMVFSRFEVRVEGSSLQATAWGES
                     IEPERHRPAVEVKAATYHALAVRREPDGGWTAQCVVDV"
     gene            7820..>8235
                     /locus_tag="PK634_11875"
     CDS             7820..>8235
                     /locus_tag="PK634_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOW98476.1"
                     /translation="MNLSRLARVSDFEWRLEPSGPMRVPGVLFADETLLAEMDDKVTE
                     QVANVAALPGIVQASYAMPDAHWGYGFPIGGVAAFDADRGGVVSCGGVGFDISCGVRT
                     HLTGLARDEVVKARERLADALFHKVPAGVGSTGPVRL"
BASE COUNT         1317 a         2678 c         2815 g         1425 t
ORIGIN      
        1 cggctgggcc gcgctggcgg tcccggcggc gcccaccggg gtcaaggcca gcgacggcac
       61 ccacgaggac cgggtggaga ttcaatggaa cgcggtgggc ggcgccacgc tgtaccaggt
      121 ctggcgcaac gattccaaca gcttgaacgg cgccgcgtcc atcaagaacg tggcggggca
      181 cctgaacagc gcgaacgatt acgatgccac gcccggccag ggctattact actgggtgcg
      241 cgcggaaaac cagggcgggt gggggcccta cagcggcagc gattcagggc atgtcgcggt
      301 cgcggccgcg ccgccggagg cgcccgacac gctgtgggcc agcgacggga agtacaccaa
      361 caaggtgtat ctcatgatgc agatcccccc ggacaccacg ggatttgata tctggcgcgc
      421 ctatcaggat aacgtggatg ccgccgccaa gattgtcgaa aaccacccga gcggggagta
      481 cagcgacttc gatgtgaccc cgggcctgta ttacttttac tgggttcaag cccggaatgc
      541 cgacggcgtg agcgggttca gccccggcga cagcggctat gccagcaacg cggtccacgc
      601 gcctgcgaag ccgccctgca tctccgccag ccagggcaca tacctggacc gcgtgacgat
      661 cgagttttgc ccggtcgagt atcggacggc gtatcactat gaaatatggc gcaatgccgt
      721 ggacaacccg gccggcgcga cgtgctttgc cattctggat cccacggagt cggcggccac
      781 cgattccaac ctggagcccg gcacgaccaa ttactacttt gtcacggcca cgaatcaata
      841 tggccgcggc cccttcagcg acagcgccaa gggatacacc atgcccggca cgccggcgcc
      901 caccaacctg gtggcttcgg atggtaccca cgtgggatcg gtcctggtgt cgtggaacgg
      961 cgtgggcacc acgtatgcct ccgtttggcg ccatacgaac aatacttccg cggcggccac
     1021 ctgcattgcg ccgaaccaga accaacccta tcacattgat tacagcgcca cgccgggcca
     1081 ggtgtattac tactgggcca aggccttttc ggggtcggtc acaagcggat tcagcaacgt
     1141 ggacaccggt tatgccggcg cggacaaccc gcccccggcc ccgggtaacg tgcaggccac
     1201 gcggggcgtg tacaataact acatcggcgt aacctgggac aaggtcctgg acgcggactg
     1261 gtatgaggtc tggcgcaaca ccagcgagga cacggccacg gccacgcgca gggacggcca
     1321 tacgaccaac aacgtgtcct ggaccgatcc ggcgccgtcc tggctgatcg ggtattacta
     1381 ctgggtgcgc agcgtgaatg agaacggcac gagtgcgtgg tcctcggcgg cctgcggcta
     1441 catggcggga gccatgtcgg tgccggaaat gcgcgtggcc accaggctgg gcaacatctg
     1501 ggtgatggcc atgaccaacc tgaccgtcgg cctgaccaac tatattcaga gcaccaccaa
     1561 tctcatgccg ggctcccaat ggatacagcg cggggcgatc accccctccc gggaccggtc
     1621 gttctggata ggcctgatgc cgggcgaaag acgtttttac cgcattgtcg tgaatccgcc
     1681 ggacggccct tgattcgtgt cggcgaggtg cgccggacgg cgggacacca caagccttgt
     1741 acatgatcgt ggtggatcgt cacggggagg gaatcatgaa tatccatccg gcgcggcttc
     1801 cgctcctggc cctcgttctc tcctgctgcg gcctcgcctc ggcacaggtt cccagcctgt
     1861 tgaattacca gggccggctc gtgaacggga ccaacctctt caacggcgtg atctcgccga
     1921 tcttccgcct ctacgtcctg ccggccggcg gctttcaaat ctacatcgag caggacctga
     1981 atgtcccggt cgccgacgga ttctattcca cgctcctcgg cgacaacttc gtcggcactc
     2041 cgctcgccga ggccttgacc aacaacccgg tgtacctgga ggtcgagatc ggcggggtcc
     2101 gcctggcgcc ccgcgagcgc gtcatatccg gcgggcgccc cctcggctcg ctggcgtacc
     2161 gcgggggcga cgcgttcgtc gtcgtgtccg tgaccaacag cgataccgtg aacgcgcaga
     2221 acctcctggc ggcctacacg gtcgccacga accggcggcc gcacgggctg cccttgagcg
     2281 ccgtcaaccg ggcggtcgtg ctggtgccgc cgggccggta cgacctcggg acgggccagc
     2341 tcacgctgtc ctgcgacttc gtggacctgg tcggtctttc ctcggcccgc gaggaccagc
     2401 atatccgggg cgagtcggac ggcgacaaca ccggcgtcct gcggcagacg gccgacgacg
     2461 cgcggatcga gaacctgttc gtggagtgca cccgctacag cggcggcacg gacgcggaca
     2521 gcgcggatcc cgccgcctat ttcccggatt cgggccggtc caacacggtg gtgcgcaact
     2581 gccgcttcaa ggccgtgggc gagggcgcga gctggtccat gcggctgggc gtggactacg
     2641 ccggcgccta cgagaactgc gccggcgagg cgtacgcctt cggcggcggc cagttcgggc
     2701 gcgcctccgg cctgttcgtc gagtgcgacg gcggcgattt cagcttcggc agcggcttca
     2761 gcgcccaggc ctccggccgg ttccgccggt gcacgggcgg ggcgggcagc ttcggcgcct
     2821 ttttcgggac ggcgtccggg accttcgagg attgcacggg cggggcgggc agcttcggag
     2881 gcgtcaatgc ctcggcctcg gggacgttca tccgctgcgc gggggaggag aacagcttcg
     2941 gcgccctggg cggcagcgcc ggcggcttct tcctgcattg ctggatgaag ggcaccaact
     3001 ggaccggcgg gcagctctcc ggccgcatgg aaggctgccg ttgggaagcg ccggtgatca
     3061 ccgcgttgag cggccgggtc ttccggtccg tggtcgaggg cgtcttccgc gacgactcgc
     3121 acttcgagat cggcgaggcc agcgtgacgg tcaagccctg ccgggacgag gagctgaacg
     3181 ggcggaacct gctggccgcg tatgccctgg ccaccaacct ctggccgcac aaccgcgccc
     3241 ggagcgccag caaccgcgta gtgctcctcg tttcgcccgg cgtgtacaac ctgggctcgg
     3301 aagcgctcca gctcacggat gaatacgtgg acctggtggg cgtgtcatcc gcccgggacg
     3361 accagcgcct cgcgggctcg gccgacgtgg actatttcgg ggtcattctc cagtcggcct
     3421 ccgacgtccg gatcgaaaac ctgttcgtgg agtgcacgcg cagcaacggc ctcgccgtcg
     3481 gggatgccga cgacccggcg gcgtatttcc cggagtcagg cctttccaac accgtgatcc
     3541 gtaactgccg gttcaaggcc aacgacacga acgcctggtc catgcgctgc ggcatcgagt
     3601 acgcggggac ctacgaggac tgcgcggggg ggaagcacag tttcgggggg aagggcgggc
     3661 aggcggcggg aatcttccgg aactgcgcgg gtggcgacta cagctttggc ttcagttcgg
     3721 ggggcgaggc ctcgggcatg ttcatggact gcacaggcgg cgccgccacc ttcgaaggga
     3781 tggccgacgc ccgggggacc ttcgatcgtt gcacgggggg aagctacgcc ttcggcggcg
     3841 gaggtcatgc caccggcacc taccggaatt gccgcagcga cgacagcgcg tttgggggag
     3901 gggggggcac ggcggggggg acgttcagcc attgcatcgg gggaaacaat tgctttggga
     3961 gccgcggagg cgccgcctcc ggtttcttcc tcaattgcgt ggcgggagac gacagcttcg
     4021 gcagccatct cggctgttac gcgtcgggaa ccttcatcaa ctgccatgcc ggcgccatgt
     4081 cgttcggcat ggatggaatc tgttcaggca ccttcatcgg ctgccgcggc gggctgcgct
     4141 cgttcggggg caacaacgat gagattacgg ggaacctgta caactgctgg atggattccg
     4201 acacgtggac aggcaacttc ggcgggcgga tggagggctg ccggtggggc gccgggatca
     4261 tggtttcgag cggcgcggtg atctacgcct ccacgttcgc cggcgcggtc acgaactttg
     4321 gcccgggatc ctgccagatc gcccacgtcc ggtgccgggc cctggacctg gggcccatgg
     4381 tcaacgcgat cgccacgccg tacaacgtcg tggacgcgga cgtggaataa gaggcccggc
     4441 ctactttctg cccgccagcg gcatgtacgc gtcgcgggtg tagtcctgca gcatccgtgc
     4501 ggcgctgaac tcggggatga tggtggccat ggcctccttc atcatcgcca cccagttcgc
     4561 cgggacgccc ttctggcggt ggtcgtagaa ggcgggtacg atctcgtttt ccaggatgtg
     4621 gtagaacgag gtcgaatcct cgtagtcctg catgtcctcg ttgtcgaagt agcggccggc
     4681 gttgatggtc cagccgttgc gcccgttggc ggcctcgggc caccagccgt cggagatgct
     4741 cacgttcagc gcgccgttca tcccggcctt catcccgctg gtaccgctgg cctcgcgcgg
     4801 gcggcgcggg gtgttcagcc agatctcgac gccctggacc atgtgccgcg cgatgcggat
     4861 gtcgtagtcc tcgacgaagt agatgtggcc gcgcaggtcg gaatggaacg cgatgttgta
     4921 gatgtgccgg acgaggtcct ggcccggctg gtcggctgga tgggccttgc cggaaaacag
     4981 gatgtgcacc gggcgctcgg gattcgtcag gattgcgcgc agccggttgt aatcgcggaa
     5041 gatcagcgcc gcgcgcttgt acgtggcgaa gcgccgcgca aacccgatca gcagcacctt
     5101 gggatcgagc atccgctcca cctcgcgcat ctcgtccggg ctgttgccgt gccgggcgaa
     5161 ctgctcgacc agccggtggc ggaacagccg gatcaggcgc tccttctggt ggatatgggc
     5221 cgcccacagg tcgtcgtcgc ggatcttctt cacgccctgc cagaattccg ggtcgcagca
     5281 ccggtcctgc cagccgtggc ccagggattc ttcgaccagt tcgcggatgt cgtgcccgag
     5341 ccaggtcgcc acgtggacgc cgttggtgat cgccttgatc ggcttgctgt cgtgcggcgc
     5401 ctgcgggaac aggtgccgcc acatgtcgtt ggacacgtcc ccgtgcagtt ggctgacgcc
     5461 gttcgagtac gaactcatcc ggatcgccag ggccgtcagg ttgaacggct ggccgcctcc
     5521 gcccgggtag gccttgccga gttccaggag ccggtccatg gccacgccca tgcccttcgc
     5581 gtgcgggccg aggtacttct cgaccagatc catctcgaac gcctcgttgc ccgccgggac
     5641 gggcgtgtgc gtggtgaaca tcgtgtccct gcggatggtg ctcatcgcgt cgtcccaggt
     5701 ccggccggcc ttcatcagct cgcgcaggcg ctcgaacagc aggaaggcgg agtgcccctc
     5761 gttcaagtgc cagacggccg gcttgatctt catctcgcgc aggatgcgcg cgccgccgac
     5821 gccgaggagc agctcctggc agaagcgcat ctcgcggccg cggacgtaga gctgcgaggt
     5881 gatggggcgg tccgcggcgt cgttctccgt gatgtccgta tccagcaggt agagcttcac
     5941 gcgcccgatc atgagctgcc agacgcgcgc cttgacctcg cggcccggga actcgacgcc
     6001 gatcaggagg ttgcggccgc tggccgtgtg cacggggcgc aggggcatgc ggtagaagtc
     6061 ggactcgttg aagaggtggt gctgccgtcc gtcgttgtcg atgttctggc ggaagtaccc
     6121 gtgacggtac agcaggccga tgccgatgaa cggcaggccc aggtcgctcg cggctttcgc
     6181 gtggtcgccg gagagcacgc ccaagccgcc gcagtagatg cccaggctct cgtgcaggcc
     6241 gaactccgtg ctgaagtacg cgaccggccc gcccttgtaa tcggggtagg ccttcttgaa
     6301 cggcgtgtcc gcctcggggt ccatgtaacg gtcgaactcg cggatcaccc ggcggtactc
     6361 ggacatgaac gagccgccgt cgctcaacag ggtctgccag tggtgcggct cgatgtcgat
     6421 cagcagctgg acggggttgc ggtacagcgc ccacagcggc tggctgatgt tgctgaacat
     6481 cgcccgcgcc cgcggcgtcc acgaccacca caggttatag gccagctcgc gcagccggga
     6541 aaactcctcc ggcatccgga tgtccggcat ggaggtcggt tcaaaagtca tggcgaaacc
     6601 ctttcgtaga acgtttcggc aattctgccc tgcgtcgaat gcgcggtagg ttaggacctg
     6661 cgttccggag tgcaagctga aagttcggcg cctcctcaaa aaagcattgc gcggcggccg
     6721 gaatccgcat acataaatcc cacgcccgtc ccgggtcggg agggcaacca caacatggag
     6781 aaccatatcg tgcaacgcat cgtcgcgtcc gtgatcgcag ggctgtgcct ggccgtgtcc
     6841 gctcccgccg tcatgctgga ggaaggcacc caggaaatcc aggtgaacgg cagtttcgac
     6901 ttcgaggggc tgggcggctc cgtgctcgat ctcggcctgg gctggggcta cttcgtccgg
     6961 gactacgtcg aggccggctt gctcggcgag gtcaccttga gcgaccccgg cgacagctac
     7021 tccatcggcg ggttcggcga gtaccatttc gacatcgagt ccatcgtggt cccgtacatg
     7081 ggcgccgggc tctccctgct catgacggac atccagaacg gcgacgacac caccgccctg
     7141 gccgtcagcg gcctggccgg cgcgaagatc tacctcgtgg aaaatgtcgc cctcgatatc
     7201 cgggcggtcc tgtccatcgc ctccgacgac gtctacatcg cggacgacga gctcgacaac
     7261 tgggacgcgc ggttcgagat gggcctgcgg ttctactatt gatccaacct ggatggggga
     7321 gggccgcgcg tcgacgcggc cctttccttt atgaccgccg cccgctggga gcatttcgag
     7381 caccaggccg atgtcggcct gcgcggccgc ggcggcacgc cggacgaagc cttcgcgcag
     7441 atcgccctcg cgctcaccgc tgtcgtcacc gatcctgatc gcgtccgggc cgagcggccc
     7501 gtggagctcc gttgctcggc ttcggacgtg gaatcgctgc tcgtggactg gctcaacgcc
     7561 ctcgtctacg agatggccgc ccgccgcatg gtcttctccc gcttcgaggt gcgcgtcgag
     7621 ggttcctcct tgcaggcgac ggcttggggt gagagcatcg agccggaacg gcaccgcccc
     7681 gccgtcgagg tcaaggccgc cacgtaccat gccctcgcgg tccggcggga gcccgacggc
     7741 ggctggacgg cccaatgcgt cgtggatgtg taggggttgg acgttggaag ttgggcgtcg
     7801 gttgttggac attgaaatca tgaatctctc ccgccttgcc cgggtttcgg acttcgaatg
     7861 gcgtctcgag ccttcgggcc ccatgcgcgt gccgggcgtg ctcttcgcgg acgagacgct
     7921 gctggccgaa atggacgaca aggtgaccga gcaggtcgcg aacgtcgccg cgctgcccgg
     7981 gatcgtccaa gcctcctacg cgatgcccga cgcccactgg ggctatggct ttcccatcgg
     8041 cggcgtagcg gcgttcgacg cggaccgggg cggggtggtt tcctgcggcg gggtgggatt
     8101 cgacatttcc tgcggcgtgc gcacccacct gaccgggctg gcccgggacg aagtggtgaa
     8161 agcgcgcgag cgcctggccg acgccctttt ccacaaggtt cccgccggcg tcggcagcac
     8221 cggccctgtt cgcct
//
LOCUS       DAOOTK010000125         7900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5736_length_7900_cov_6.006501, whole genome shotgun sequence.
ACCESSION   DAOOTK010000125 DAOOTK010000000
VERSION     DAOOTK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5736_length_7900_cov_6.006501"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PK634_11880"
     CDS             complement(<1..714)
                     /locus_tag="PK634_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98477.1"
                     /translation="MTAGGEPFAEGAAEYELSLVCELRAVKERSPAFSTPLLVKNAGW
                     FCRLRWIVVAMLLLFGLLVRFPPIASFFGVPSAGHWPWACAAVLGIGNVVFLWHGGRA
                     AREGRRRLVAANLWAQILLDLVILTLVVQFLGGARSAFAFAYLFHIVLACIFLPRLQS
                     LLVTLLACLLYLAGTAGAQAGLFGGGLVDAAPGRVGLAVVVTAGIWLVVWYLTSQITE
                     TVRARDLELSRTNERLLRAQ"
     gene            842..2257
                     /locus_tag="PK634_11885"
     CDS             842..2257
                     /locus_tag="PK634_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98478.1"
                     /translation="MTTQDSSPAVRPGDSLLSILLAIFRLAGPAVLAMLLAFPLYKGR
                     PSGWLPLSVAAAVAGLLLFRKTRLPSLPPRRFLALVLGIPALLQAALVLWLRPDPAFD
                     GLFVLQEAERLARTGEMSALTYYAPGQIWYYALLFKIFGASPLVAQLGQIPLFLGMIL
                     ALHAIARRALPPLPARAATLAMAYYPSMILYALVTPYYFYLYTLMILLMAGRWLAVAR
                     GGGAGAGFAGGLAAGFGALTKAVLLVAPAQALAFWLLSARSFFQRRVWAAWLAFILGL
                     LIVIAPWAWRNRQVLGEPVLVCTSGPLVLWSANNPDSDGLYSALPDMTRIETPEEMLR
                     HMRYCRDQAKAFILFHPGQFIRLAGRKLLHTWGTETSYVELINRGGAPLGPLDPAFRF
                     IAQVGWGTLVWLWAFVAARALIRRRPATPVELATAILVLSKWVIYSFYEGGARHHLPV
                     VPMLILYVVSELAADRSSEIA"
     gene            complement(2209..3192)
                     /locus_tag="PK634_11890"
     CDS             complement(2209..3192)
                     /locus_tag="PK634_11890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW98479.1"
                     /translation="MRLLTNMAFWQCPYWTERVDCLYDLAGAKADPAFMPFWKEAWRL
                     FRKSRAYDAVLTMESRTSLAYGWLCSVAGRPSKQIMTEVFIDQPRPASLFWRLKTALF
                     RWIARRGVGALVLSSAELETVSERFGLPRKKLRFIPLQCTIEQPREAPADEGFVLSAG
                     RSLRDYGTVIRAAAGIRAPVLILCGPADDIPAGPASVTVEREATRERYMDALARCSVV
                     ALALQDTLRPTGQVVMLEAMGLGKPVVVTRHAGTVDYIRDGENGFLVEPGDAEGLARK
                     VRMLLDDPALRRRIGRQALADVMEKYSLEAHAKARLQAISELLSAASSETT"
     gene            complement(3226..4338)
                     /locus_tag="PK634_11895"
     CDS             complement(3226..4338)
                     /locus_tag="PK634_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98480.1"
                     /translation="MPGPFQVMFVTPAFVYGGLERVILDLVKALDRERFRPMICSLYP
                     PAPGMKPRLDAAGVPFFAFDKGDGVNLRLVGQLARLFRRERVDLVNAHDIGATFYAGP
                     AARLAGIRRVVHTDHSQVLGLRRRLGLFGAVLRHTAARATTVSEHLRRFLVEKLHYPA
                     ARAQVIPNGMDLSAYEKSEPGLRPEFGFGPADKVIGACGRLTEQKGMLHLLRALPGLL
                     KTQPAARLLIVGDGPQRADLAAEAARLGVGDRVTLTGNRDDLPGLMRTMDVFALPSLW
                     EGQPLVLLEAMAAGVPIVATDVGGDAEVVGGGKYGALIPPADEAALTEALRQALEQPE
                     PARRRAEQARAHVFADRTHTAMARQYESLFENLLSH"
     gene            complement(4348..5616)
                     /locus_tag="PK634_11900"
     CDS             complement(4348..5616)
                     /locus_tag="PK634_11900"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOW98481.1"
                     /translation="MSAYNPWEMACQQLDTVARRIGLDPNIHAKLRNCKRALIVSVPI
                     QMDDGQVRVFEGYRVQHNLERGPAKGGIRYHPDVTLDEVKALAMWMTWKCAVVGLPYG
                     GAKGGVVCDPNRMSLAEIERLTRRYTSEISIIIGPEKDIPAPDVNTNPQVMSWIMDTY
                     SMNVGYSAPGVVTGKPIDIGGSEGRLTATGRGAMIVIREALQHLNMRPDQCTVAVQGF
                     GNVGGATAQFLHELGCRVVGVSDAFGGVFNPKGLDIPALARHCRTSERHAVAGFDGGD
                     VVEGGAKATEELFKLEVDILAPCALENQITKDNARAIRAKIVAEGANGPTTPEADEIL
                     CRKGVFMLPDILANAGGVTVSYFEWVQDIQAHFWQLEDINKELARIMTKGFHDVLEIA
                     EREKCDMRMAAYILAVSRVATAVRLRGIYP"
     gene            5775..6155
                     /locus_tag="PK634_11905"
     CDS             5775..6155
                     /locus_tag="PK634_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOW98482.1"
                     /translation="MPRPCCPRKVGFVPGVTYFKPAGVPLRVLEEVVVALDELEALRL
                     ADLEAMYQEQAAARMNISRPTFARVVEAARRKVADALIHGKALRLEGGPVHPAAVSPP
                     PGSGRGCGRHGRGWQDGRKERNGG"
     gene            6152..6514
                     /locus_tag="PK634_11910"
     CDS             6152..6514
                     /locus_tag="PK634_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOW98483.1"
                     /translation="MKIAVTAQSENPNGALDPHFGRAKKFVVFDTETGQYTAHDNAQN
                     LNAVQGAGIQAAQAVARLGAEVLLTGHVGPKAFRTLAAAGIQICLAPPGTVAAAIEAW
                     KAGALKETTGANVEGHWV"
     gene            6539..6736
                     /locus_tag="PK634_11915"
     CDS             6539..6736
                     /locus_tag="PK634_11915"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOW98484.1"
                     /translation="MPRGDGTGPQGQGARTGRGMGRCGGNSSQGAAESTPPQGRGQGG
                     ARSGRQGQVRGRGRGQGRGRS"
     gene            6756..7073
                     /locus_tag="PK634_11920"
     CDS             6756..7073
                     /locus_tag="PK634_11920"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOW98485.1"
                     /translation="MPGGDRTGPRGMGAMSGRGAGHMNGGFGRGFGRGQGGPSTRFGA
                     GGRGWRNMYFATGLPGWIQTPTAPAPEQELAALKQQADNFGKALEDIQGRIRKLESKP
                     ADQ"
     gene            7120..>7900
                     /locus_tag="PK634_11925"
     CDS             7120..>7900
                     /locus_tag="PK634_11925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOW98486.1"
                     /translation="MTAFRHLFGPVPSRRFGRSLGVDLTPLKTCTLDCVFCQLGHTSC
                     KTLERREYVPVAEVTTELARWRDEGGQADYITLAGSGEPTLHSGFGDVLEFIRDELPV
                     PAVLLSNGTLFWESDVREAACQARIVKLSLSAWDTRTFQRVNRPHPELSFDVVVAGLR
                     TFRQMFPGTIWLEVFLVDGLNTTAEGLERLARLAETIAPDEIHLNTAVRPAAERAVQA
                     VPRERMEEWAGWFRPRATVIAGFPKRSGVNIEAGERAILDML"
BASE COUNT         1263 a         2720 c         2711 g         1206 t
ORIGIN      
        1 ctgcgcccgc aggagccgct cgttcgtccg cgacagttcc aggtcccggg cgcggacggt
       61 ctcggtgatc tgcgaggtca ggtaccatac gaccagccag atccccgccg tcacgaccac
      121 ggccaggccg acccgccccg gcgcagcatc caccagcccg ccgccgaaca ggcccgcctg
      181 ggcgccggcc gtgcccgcca ggtacagcag gcaggcgagc agcgtcacca gcaggctctg
      241 caggcgcggc aggaagatgc atgccaggac aatgtgaaag agataggcga acgcgaacgc
      301 gctccgcgcc ccgccgagga attggacgac cagcgtgagg atgaccaggt cgagcaggat
      361 ctgcgcccag aggttcgcgg ccaccaggcg gcggcggccc tcccgcgccg cgcgcccgcc
      421 atgccagaga aacacgacgt tgccgatccc cagcaccgcc gcgcaggccc acggccagtg
      481 accggcggag ggaactccga agaaactcgc gataggcggg aaacggacga gcaggccgaa
      541 gagaagcagc atcgccacca cgatccaccg caggcggcag aaccagccgg cgtttttcac
      601 cagcaggggc gtcgagaacg ccggactccg ctctttcacc gcgcgcagtt cgcacaccag
      661 cgacagctca tattccgccg cgccctcggc gaagggctcc ccgccggccg tcatcggacc
      721 gccccctcgt gatgccacgc gttcacacgc ccggaaatgt agcccgcccg ccctgccctg
      781 caagccggaa aacgattgct ctttgcgcgg cgaagcaact tccctactat gcccgccagg
      841 catgaccacg caggattcca gcccggccgt gcgcccaggg gattcgctgc tttccatcct
      901 gcttgcgatt ttccgcctcg ccggcccggc cgtgctcgcc atgctgctgg cctttcccct
      961 gtataagggt cgtccgagcg gatggcttcc gctgtccgtt gccgcggccg tcgcgggact
     1021 gcttctcttc cgaaaaaccc gcctgcccag cctcccgccg cgccggtttc tcgccctggt
     1081 cctcggaatt cctgcgctgc ttcaggcggc actggtcctc tggttgcggc ccgatccggc
     1141 attcgacgga ctcttcgtgc ttcaggaagc cgagcgcctg gcccgcacgg gcgagatgtc
     1201 ggccctcacc tactacgccc ctgggcaaat ctggtactat gccctgctgt tcaaaatatt
     1261 cggcgcgtcg ccgctcgtgg cgcaactggg ccagattccc ctgtttctcg gcatgatcct
     1321 cgcgctgcac gccatcgccc gccgcgccct gcccccgctg cccgcgcggg ccgccacgct
     1381 ggccatggcg tactacccct ctatgatcct ctacgcgctg gtcaccccgt actacttcta
     1441 tctctacacg ctgatgatcc tgctgatggc cggccgttgg ctcgccgtcg cccgaggagg
     1501 cggcgccggc gcgggcttcg cgggcggact ggccgcgggc ttcggcgcgc tgacgaaagc
     1561 cgtcctcctg gtcgcgcccg cccaggccct cgcgttctgg ctgctgtccg cccggtcgtt
     1621 cttccaacgc cgcgtgtggg ccgcgtggct cgcgttcatc ctcgggttgc tcatcgtcat
     1681 cgcgccatgg gcgtggcgga accggcaggt gctcggcgaa ccggtcctcg tctgcacctc
     1741 cggcccgctg gtcctctgga gcgccaacaa cccggattcc gacggcctct acagcgccct
     1801 gcccgacatg acgcgcatcg aaacgccgga ggaaatgctc cggcacatgc gctactgccg
     1861 ggaccaggcg aaggccttca tcctcttcca cccgggacag ttcatccgac tggccgggcg
     1921 caagctgctc cacacgtggg gcacggagac cagctacgtc gagttgatca accgcggcgg
     1981 cgccccgctg ggtccgctcg atccggcctt ccggttcatc gcgcaggtcg gatggggaac
     2041 gctggtatgg ctctgggcct tcgtcgcggc gcgggccctg atccggcgtc gcccggccac
     2101 gcccgtcgag ctggcgacgg cgatcctggt cctgtcgaaa tgggtgatct acagcttcta
     2161 cgagggcggc gcgcggcatc acctgccggt cgtgccgatg ctgatccttt acgtggtctc
     2221 ggaactggcg gccgacagga gctcggagat cgcctgaagc cgggccttcg cgtgggcctc
     2281 gagactgtat ttctccatca cgtcggccaa ggcctgccgg ccgatgcgcc gccgcagcgc
     2341 cggatcgtcc agcagcatgc ggaccttgcg cgccaggcct tccgcgtcgc ccggctcgac
     2401 caggaacccg ttctctccgt cccggatata gtccaccgtc ccggcatggc gcgtaacgac
     2461 caccggcttg cccagcccca tggcttccag catgaccacc tgccccgtcg gccgcagcgt
     2521 atcctgcagc gccagggcca cgacggagca ccgggccagc gcgtccatgt agcgttcgcg
     2581 ggtcgcctct cgctcgaccg taacgctggc cgggcctgcg gggatgtcgt cggccggccc
     2641 gcacaggata agcacgggcg cccggatccc cgccgcggcg cggatcaccg ttccgtaatc
     2701 gcgcagcgag cggccggcgg aaagcacgaa cccctcgtcg gccggcgcct cgcggggttg
     2761 ctcgatggtg cattgcaggg gaatgaagcg cagctttttc ctcggcaggc cgaatcgctc
     2821 cgacacggtt tccaactccg cggagctcaa gacgagcgcg ccgacccccc gccgcgcgat
     2881 ccagcggaag agcgcggtct tcagccgcca gaaaaggctc gcgggccggg gctggtcaat
     2941 gaacacctcg gtcatgatct gcttcgaggg gcggccggcc acggagcaca gccacccgta
     3001 ggccagcgag gtgcgggact ccatcgtcag cacggcatcg taggcccggc tcttccggaa
     3061 cagccgccag gcttctttcc agaacggcat aaaggccgga tccgccttcg cgccggccag
     3121 gtcgtagagg cagtccacgc gctccgtcca gtacgggcat tgccagaagg ccatgttggt
     3181 caggaggcgc atggggaagg attcagggag ctattcacta gttccttaat gggaaagcaa
     3241 attttcaaag aggctttcat actgccgcgc catggcggtg tgcgtgcgat cggcgaacac
     3301 gtgcgcgcgg gcctgctcgg cgcgccgacg cgcgggctcc ggttgttcca gcgcctgccg
     3361 cagggcttcc gtcagcgcgg cctcgtccgc gggcgggatg agcgcgccgt acttgccgcc
     3421 gccgaccacc tccgcatcgc cgccgacatc ggtcgcgacg atcggcacgc ccgccgccat
     3481 ggcctcgagg aggaccaggg gctggccttc ccacagcgaa ggcagcgcga agacgtccat
     3541 ggtccgcatc aaccccggca ggtcatcgcg gttcccggtc aacgtgacgc gatcccccac
     3601 cccaagcctc gccgcctcgg ccgccaggtc cgcccgctgc gggccgtcac cgacgatcag
     3661 caggcgggcc gccggctgcg ttttcagcag gccgggcagg gcgcgcagga ggtgaagcat
     3721 gcccttctgc tccgtcagac ggccgcacgc gccgatgacc ttgtcggccg ggccgaatcc
     3781 gaattccggg cggaggccgg gctccgactt ctcgtaggcg gacaggtcca tgccgttcgg
     3841 aatgacctgc gcgcgcgcgg ccgggtaatg cagtttctcc acgaggaatc gacgcaggtg
     3901 ctcggagacc gtcgtggcgc gcgccgcggt gtggcgcagg acggcgccga acaggcccag
     3961 ccgccggcgc aggccgagca cctggctgtg atcggtgtgc accacgcgcc ggatgcccgc
     4021 cagccgggcc gcgggtcccg cgtagaacgt cgcgccgatg tcgtgcgcgt tgaccaggtc
     4081 cacgcgctcg cgccggaaga ggcgcgccag ttgtcccacc aggcggaggt tgaccccgtc
     4141 gcccttgtcg aaggcgaaaa acggaacgcc cgccgcatcg agccggggct tcatgccggg
     4201 cgcgggcgga tagagggagc agatcatcgg ccggaaccgt tcgcgatcca gcgccttgac
     4261 caggtcgagg ataacccgct ccagcccgcc gtagacgaag gcgggcgtga cgaacatcac
     4321 ctggaacggg ccgggcatgt caggccgtca cgggtagatc ccgcgcaggc gcacggcggt
     4381 ggcgacgcgg ctgacggcga ggatataggc cgccatgcgc atgtcgcatt tctcccgctc
     4441 ggcgatctca aggacgtcgt ggaagccctt ggtcatgatc cgggccagtt ccttgttgat
     4501 gtcctcgagc tgccagaagt gggcctgtat gtcctgcacc cactcgaagt aggaaaccgt
     4561 cacgccgccg gcgttggcca ggatgtccgg gagcatgaac acgcccttgc ggcacaggat
     4621 ctcgtccgcc tcgggtgtcg tgggcccgtt ggcgccttcc gcgacgatct tcgcccggat
     4681 ggcgcgcgcg ttgtcctttg taatctggtt ctccagcgcg cagggggcca ggatatccac
     4741 ctccagcttg aacagttcct cggtggcctt cgcgccgcct tccacgacat cgccgccgtc
     4801 gaatccggcg acggcgtgcc gctcgctggt ccggcagtgg cgcgcgagcg ccgggatatc
     4861 caggcccttc gggttgaaga ccccgccgaa ggcgtccgag acgccgacga cccggcaccc
     4921 gagttcgtgc aggaactggg cggtggcgcc gccgacgttg ccgaagccct ggacggccac
     4981 ggtgcactgg tccgggcgca tgttcaggtg ctgcagcgcc tcgcggatca cgatcatggc
     5041 gccccgcccg gtcgcggtca ggcgcccctc gctgccgccg atgtcgatcg gcttgcccgt
     5101 gaccacgccg ggcgcggagt agccgacgtt catggaatag gtgtccatga tccaggacat
     5161 cacctgcggg ttcgtgttca catccggcgc ggggatgtcc ttctccgggc cgatgatgat
     5221 ggagatctcc gaggtgtacc gccgcgtgag ccgctcgatc tcggcgaggg acatccggtt
     5281 gggatcgcac accaccccgc ccttcgcgcc tccgtacggc agcccgacga cggcgcactt
     5341 ccaggtcatc cacatcgcca gcgccttgac ctcgtcgagc gtcacgtccg ggtggtagcg
     5401 gatgccgccc ttggccgggc cgcgctcgag gttatgctgg acgcggtagc cctcgaacac
     5461 gcggacctgg ccgtcgtcca tctggatcgg gacggacacg atcagcgccc gcttgcagtt
     5521 ccgcagcttc gcgtggatgt tggggtccag accgatccgc cgggccaccg tatccagctg
     5581 ctggcacgcc atttcccaag gattgtatgc gctcatgggg aacctcctgg gccgcgggaa
     5641 aagccccgcg cgccatggtt gaacgacatc atgccgccat tatggcgcgg gcccccgcag
     5701 agtcaagaac cccgcccttg acaggtattt attatgagca tatgctcatt atgaattcaa
     5761 gccaggatgg acccatgccg agaccgtgct gcccgagaaa agtgggcttc gtgccggggg
     5821 tgacgtactt caagcccgcg ggggtacccc tgcgcgtgct ggaggaggtc gtcgtggcgc
     5881 tcgacgagct cgaggcgcta cgcctggccg acctggaggc gatgtaccag gagcaggccg
     5941 ccgcgcgaat gaacatctcg cggccgactt tcgcccgcgt ggtcgaggcc gcccggcgga
     6001 aggtcgccga tgcgctgatc cacggcaagg cgctacggct ggaaggcgga ccggtacatc
     6061 cagcggccgt gtctccgccg cccggaagcg gccggggatg tggacgacat ggacgcggct
     6121 ggcaggacgg aaggaaagaa aggaacggag gatgaaaata gcggtcacgg cgcagtcgga
     6181 gaacccgaac ggggcgctcg acccgcattt cgggcgggcg aagaaatttg tcgtcttcga
     6241 cacggaaacc gggcagtaca ccgcgcacga taatgcgcag aacctgaacg ccgtgcaggg
     6301 cgcgggcatt caggccgccc aagccgtcgc gcggctgggc gcggaagtct tgctcacggg
     6361 gcacgtcggc ccgaaggcgt tccgaacgct ggcggcggcg ggcatccaaa tctgcctcgc
     6421 gccccccggc acggtggctg cggctatcga ggcctggaag gccggcgcat tgaaggaaac
     6481 cacgggcgcc aatgtcgagg gacattgggt ctgatcacaa cggaaaggaa tcaaggacat
     6541 gccaagagga gatggaacag gcccgcaggg gcagggtgcg cgcacgggtc gcggaatggg
     6601 ccggtgcgga gggaactcgt cgcagggcgc cgcggaatcg acgccgccac agggacgcgg
     6661 ccagggcggc gcaagaagcg gccgacaggg ccaggtccgc ggccggggcc gcgggcaagg
     6721 tcggggcaga tcataacagg aaaggagagt gcagtatgcc aggtggagac aggacagggc
     6781 cgaggggcat gggcgcgatg agcggacgcg gagccgggca catgaacgga ggctttggcc
     6841 gaggcttcgg ccgcggccag ggcggcccct cgacaaggtt cggggcaggc gggcgcggat
     6901 ggcgaaacat gtacttcgcg accggcctgc cgggctggat tcaaacaccc acggcgccgg
     6961 cgccggagca ggaattggcc gcgctcaagc agcaggcgga caacttcggc aaggcgttgg
     7021 aggatatcca gggccgcatc cggaagctcg agagcaagcc ggcggaccag tagcacgggc
     7081 gttgcgccgg gcgggcgtcc gcccgcccgg caggaaccca tgaccgcttt ccgtcatctt
     7141 ttcggcccgg tgccctcgcg ccggttcggc cggtcgctcg gcgtggacct gacgccgctg
     7201 aagacgtgca cgctggactg cgtcttctgc cagctcgggc acacgtcgtg caagacgctg
     7261 gagcgccgcg agtatgtgcc ggtcgccgag gtgaccaccg agctcgcgcg ctggcgcgac
     7321 gagggcggcc aggcggacta catcacgctc gcgggctcgg gcgaaccgac gttgcactcg
     7381 ggcttcgggg acgtcctgga gttcatccgg gacgagttgc ccgttcccgc ggtgttgttg
     7441 tccaacggca cgctgttctg ggaatccgac gtccgggagg cggcctgtca ggcgcggatt
     7501 gtgaagcttt cgttgagtgc ctgggacaca cggacgttcc agcgcgtcaa ccggccgcac
     7561 ccggagttga gcttcgacgt ggtcgtcgcg gggctgcgca cgttccggca gatgtttccc
     7621 gggacgatct ggctggaggt gttcctcgtc gacgggctga acacgacggc cgaggggctg
     7681 gaacggctcg cccggctcgc ggagacgatc gcgccggacg agatccacct gaacacggcg
     7741 gtgcgcccgg cggcggagcg ggcggtgcag gcggtgccgc gcgagcgcat ggaggaatgg
     7801 gccggctggt tccggccgcg ggcgacggtg atcgcggggt tcccgaagcg atccggcgtg
     7861 aacatcgagg ccggcgagcg ggcgatcctg gacatgctga
//
LOCUS       DAOOTK010000126         7881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5753_length_7881_cov_5.123562, whole genome shotgun sequence.
ACCESSION   DAOOTK010000126 DAOOTK010000000
VERSION     DAOOTK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5753_length_7881_cov_5.123562"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK634_11930"
     CDS             <1..479
                     /locus_tag="PK634_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98487.1"
                     /translation="MTAPVTEVGVVMAKYLGALSFYVILWIPTAAYGIILHAFSPLTA
                     PVDLGPMMGGYLGALLVGAFYLSVGVLCSAVAVHQIMAAVACFAAIGVFFFTGFFAFL
                     ARSEAARDAFAYVSSVSHMLDFARGVVDTRPVALYVSLTALMLFAAVRAVEARRWK"
     gene            525..1976
                     /locus_tag="PK634_11935"
     CDS             525..1976
                     /locus_tag="PK634_11935"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOW98488.1"
                     /translation="MEPGAWRRALLRANTLLALALAAVLALMVNYLAYRHYARADWSR
                     SGSNTLSEKTRGLLDGLTGAVEVVVFLQPGQEVFEDAVHLLDEYEAASPLIHVERVDP
                     DRDLARTEELMRRYEVTEANVIVFDGGGRRKYVRMAELADYDYEPVQMGEAPRRVSFK
                     GEQAFSSALYSITQASQPVVYFLQGHGERDIESFERGAGYSAIAELVRRDHAEVKPLV
                     LGRTPAVPDDASAVVVAGPQKAFSAAELDVLGDYLERQGRLLLLLDAATATGLESLCE
                     KWGLRLVNDVVLDATRTLTGRELFITEYEPHPVTAKLNGLSTVLYLPRSVEPIEGEEG
                     GGDPADRPAAAPLASCSESGWAETDSLQSPMRYDPERDRPGPVPVAAAVEKGPVPGID
                     VQIRPTRLVVIGDSDFAANGVMTGANEDLFLSALNWLLERDELLGIAAKPVEDNRLLM
                     TQRQLRGLFWAVVGVIPALPALLGVLVWWRRRT"
     gene            1980..3836
                     /locus_tag="PK634_11940"
     CDS             1980..3836
                     /locus_tag="PK634_11940"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOW98489.1"
                     /translation="MKFKTTWMLLLAVLLVGALMWRFERGTRSSRERAEAARRALDYE
                     PADIVFLGLESTNLMVECVKEGGRWMITRPFRARADAGTVEHFLQGLRGLARGEVITR
                     RDQKGRGLTPEQYGLSESAPRLVLGTPHHRRAVRIGRVAPLGGSVYIREEGQDEIVAV
                     DQALLALLPASATDLRDRALLPGGPELVQRLEIRRGDGFLQIARGERGRWMIQQPVTA
                     RASSGEVQRFLEALFLLRAAEFLPAEMKDPAAYGFDDPGLRVSVTLSRGGEASVLVGD
                     PVDRSAARFYTLSRPDDSAGMVEAAQLEPLNVTLDDLRDRKLTTMEARDVHRVVIEAG
                     EQRVALREEAQGWSLVEPRRWNADPERMQELLSVWTAPVIQKFLPSPDAAAPAPDAEI
                     VIRMGRRADPAGAAGGDAEEETVLEIGPASATAGCRSVRVRPDGFAAEIPESLLQFVS
                     ADPLFYRDRGVLRINPDEVARLAVSRDGWTEEIRRGSSGEFESGPGGEGTRVEVEAVK
                     DLLRLLHDLRAERFLAETGAPADRYGFGPGADKLEIGLRGAAGLGNTLYFGQEAGPSG
                     RYAMIQGQEGIFELNREDCERLLPRLLSTVPVSGSAENGAHAADAAGSPAVP"
     gene            3790..6279
                     /locus_tag="PK634_11945"
     CDS             3790..6279
                     /locus_tag="PK634_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOW98490.1"
                     /translation="MEPMLQTPPAAPPSPDARASRKRPAFLFRAGRRLLRFLAGFAGL
                     VLVVSAALLLYCSAAGFPAWLVKRVLARLEEQGVHARVRRARLSVMDGIVLKGFRLYD
                     DPAAAAPVIEADRFALGLNALEWLSRRHGIVGLRIRGARLRLYLEARDDPNLSEQVVL
                     ENLHAAIRFRPGAVEMERLYAEGLGMQFRGAGTIHRRLPEKETGRSGADLLRRCPDWL
                     FKALEQVRALQFSQPPQVRLDFALELDDPAESRVELKIQSRRTQTPVGLFDRWSVDAA
                     LDGSRMMLKSLILRQGAARLNLNGALSLANRKAEFRLFSDLPLKQWMAILPRAWRLRL
                     EQMGYELKGEASLELWGGPAPWSEIKQHLGGWISLEKAECGGVWVEKAFLSVSRENGV
                     FTLEKLDGVVGRGVRQGPLKAAAVLDTASGAFHGEMEGAFNPHLWKPLLLKQQMEILD
                     WFQFGDVPPVVKLEFSGVVGDLQRFAFQGSMSATSFAYRGVGLTAGSAGLALRDGVMR
                     LDPLDIAREEGGAHGWIELDYNRQTAAFQIDSTADPHAVARMIGPGPGRFLEDYRFEG
                     PAHVTASGVVSYVGFSPVDVEAWVEGRGVGLGWALSDEAAFRVRASNQRVEITDIRAE
                     AYGGRLTGRMAFYPLNKPNDLRYDAVGQLADTRFEDLMAALIRLTENPYKGRLSADFR
                     LEGRVGEGRGRTALGEGRIRIRDGALFQIPLMGGLSEWLTRLYPGLGFASQTDFRSEF
                     LLQDGAFHTEEALLEGSVLSLKAKGDYRLDRRIDMTVQVQPLRDGVVAEAVRLATSPL
                     TKLLEFKLSGTIADPKWRPVNLPKEMFLIFD"
     gene            6357..6920
                     /locus_tag="PK634_11950"
     CDS             6357..6920
                     /locus_tag="PK634_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98491.1"
                     /translation="MSAWMHSCSTLALMALAVGLVVAGLNVPCLLWPGRVRAAALAFP
                     RSRGPAWLLTALDLGWVAWIVLHAQLGRFEWVKPYLYAAAPLSFFLIVIFMDELLAPR
                     ALGGLLLLLANPVIIAARWHESDWRLVMIGLAYVWAVAGIVLVLSPFYFRRVAAWALA
                     TDGRCRQLGLLRLAFGAALVALALAVY"
     gene            6927..7862
                     /locus_tag="PK634_11955"
     CDS             6927..7862
                     /locus_tag="PK634_11955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOW98492.1"
                     /translation="MSRPARFLVLRGGAIGDFLLTLPVFQALRARWPDAWIEVLGYPH
                     VAGLASAAGLVNRVDSLDRAGVARLFSVAPDFPEEQVRHLRSFDFVLSYLHDPTGLVR
                     ENLKSAGARVVLHGSPIVERGHAVEHLMKPLESLAIYAGGEHPSLALPGVHREAGRRW
                     FRERGLREPVLAVHPGSGSAAKNWPLDRFVEAARRIGAARHLDYFYVLGEADRGLSDA
                     LHRMDGLRPVLKEAPLLEVAGVLSGGRLYLGNDSGITHLAAAVGVPTVALFGPSDAVR
                     WGPRGPLVRVVRAPDSDLNELAVDVVLQVMDGLLA"
BASE COUNT         1061 a         2621 c         2856 g         1343 t
ORIGIN      
        1 tgatgaccgc gccggtcacg gaggtcggcg tggtcatggc caaatacctg ggcgcgttga
       61 gcttctacgt gatcctctgg atccccaccg cggcctatgg gatcatcctg cacgcgttca
      121 gccccctcac ggcccccgtg gacctgggcc ccatgatggg cggctatctc ggcgcgctgc
      181 tcgtcggcgc cttttacctg tcggtcggcg tcctgtgctc cgccgtggcc gtccaccaga
      241 tcatggccgc cgtcgcgtgc tttgccgcga tcggcgtttt cttcttcacg ggcttcttcg
      301 cgttcctggc gcgcagcgag gccgcgcggg acgcgttcgc ctacgtctcc tccgtgtccc
      361 acatgctcga cttcgcccgc ggcgtcgtgg acacgcgccc ggtggcgttg tacgtgtcct
      421 tgacggcgct catgctcttc gccgcggtcc gggccgtcga agcgcggcgg tggaaatgac
      481 gcatggaaac gcttgaaccc aggaacccgg aaaggcggtc ccccgtggaa ccgggcgcgt
      541 ggcgccgcgc gctgctccgc gccaacacgc tgctggcgct ggccctcgcg gccgtcctgg
      601 cgctcatggt caattacctg gcctaccgcc attacgcccg ggcggactgg agccggtccg
      661 gatccaacac gctatccgaa aaaacccggg gcctgctgga cggcctcacc ggcgccgtgg
      721 aggtcgtggt gtttctccag cccggccagg aagtgttcga agacgccgtg cacctgctgg
      781 acgagtacga ggcggcctcg ccgttgatcc atgtcgagag ggtggacccg gaccgcgacc
      841 tcgcacggac cgaggagctg atgcggcgct acgaggtgac cgaggccaac gtcatcgtgt
      901 tcgacggcgg cgggcgccgc aagtacgtgc ggatggccga gctggcggac tatgactatg
      961 agcccgtgca gatgggcgag gcgccgcgcc gcgtgtcctt caagggcgag caggcgtttt
     1021 cctccgccct ctacagcatc acgcaggcga gccagcccgt ggtctacttc ctccaggggc
     1081 acggcgagcg cgacatcgag agcttcgagc gcggggccgg ctactcggcc atcgcggagc
     1141 tcgtccgccg cgatcacgcc gaggtcaagc ccctggtcct cggccggacg ccggccgtcc
     1201 cggacgacgc ctccgccgtg gtcgtcgccg gcccccagaa ggccttctcc gcggcggagc
     1261 tggacgtgct gggcgactac ctggagcggc agggccgcct gttgctcctg ctggacgccg
     1321 cgaccgccac cggactggaa tcgctctgcg aaaaatgggg tctccgcctg gtgaacgacg
     1381 tggtgctcga cgccacccgc acgctcacgg ggcgcgagct gttcatcacc gagtacgagc
     1441 cgcatcccgt caccgcgaaa ctgaacggct tgagcaccgt gctgtacctg ccgcggtccg
     1501 tcgagccgat cgagggcgag gaggggggcg gcgatcccgc ggaccggccc gccgccgcgc
     1561 cgctggcctc ctgcagcgag tcgggctggg cggaaaccga cagcctgcag agccccatgc
     1621 gctacgatcc cgagcgggac cggcccggcc ccgtcccggt cgcggccgcg gtggagaagg
     1681 ggccggtgcc cggcatcgac gtccagatcc ggcccacccg cctggtggtg atcggcgact
     1741 ccgattttgc ggcgaacggc gtgatgaccg gcgccaacga ggatctgttc ttgagcgcgc
     1801 tgaactggct gctggagcgc gacgaactgc tggggatcgc ggccaagccc gtcgaggata
     1861 accggctgct gatgacgcag cgccagttgc gcggcctgtt ctgggcggtg gtcggcgtca
     1921 tcccggcgct gccggcgctg ctcggcgtcc tggtctggtg gcgccggagg acgtgagcga
     1981 tgaagttcaa gacgacatgg atgctgctgc tggccgtcct tctggtcggc gcgctgatgt
     2041 ggcgtttcga gcgcgggacc cgctcctcgc gggagcgagc cgaggcggcg cgccgggccc
     2101 ttgactacga gccggccgat atcgtcttcc ttggcctcga gtccaccaat ctcatggtcg
     2161 agtgcgtcaa ggagggggga cgctggatga tcacgcggcc gttccgcgcg cgggcggacg
     2221 cggggaccgt ggagcatttc ctccagggat tgcgcgggct ggcgcgcggc gaggtgatca
     2281 cgcgccgcga ccagaagggg cgcgggctga cgccggagca gtacggtctc tcggagtccg
     2341 ccccccgcct cgtcttgggc acgccgcatc atcgccgggc cgtgcggatc ggccgcgtcg
     2401 ccccgctcgg gggctcggtc tacatccggg aggaaggcca ggatgagatc gtggccgtcg
     2461 accaggccct gctggccctt ctcccggcat cggccaccga cctccgcgac cgggcgctgc
     2521 tgccgggcgg gccggaactg gtgcagcggc tggagatccg ccgcggggac ggattcctgc
     2581 agatcgcccg gggcgagcga ggccgctgga tgatccagca gcccgtgacg gcccgcgcct
     2641 cctccggcga ggtgcagcgt ttcctcgagg ccctcttcct gctgcgcgcg gccgaattcc
     2701 ttcccgcgga aatgaaggat ccggccgcct acggcttcga cgatccgggc ctccgggtca
     2761 gcgtgacgtt gagccggggc ggggaggcgt ccgtcctggt gggggacccg gtggaccgga
     2821 gcgccgcccg tttctacacg ttgagccggc cggatgattc cgccggcatg gtggaagccg
     2881 cccagttgga gccgttgaac gtcacgctcg acgacctgcg cgaccggaaa ctgaccacca
     2941 tggaggcgcg cgatgtccac cgggtggtca tcgaggcggg agagcagcgg gtggccctgc
     3001 gggaggaggc gcagggctgg agcctggtcg agccccgccg ctggaacgcg gacccggaac
     3061 ggatgcagga gttgctgtcc gtgtggaccg ccccggtgat ccagaagttc ctgccgtctc
     3121 ccgacgccgc cgctcccgcc ccggacgcgg agatcgtcat ccggatgggc cggcgcgccg
     3181 atccggcggg ggcggccggc ggagacgcgg aggaggaaac cgtcctggaa atcggaccgg
     3241 cctccgcgac ggccggctgc cggtcggtgc gggtgcgccc cgatggattc gcggcggaga
     3301 tccccgagag cctccttcag tttgtttcgg ccgatccgct gttctaccgc gaccgcgggg
     3361 tgctgcggat caatcccgac gaggtcgcgc gcctggccgt ctcgcgagac ggctggacgg
     3421 aggaaatccg gcgcggatca tccggcgagt tcgagtcggg cccgggcggc gagggaaccc
     3481 gggtcgaggt agaggccgtg aaggacctcc tgcggcttct ccacgatctg cgcgcggagc
     3541 ggttcctggc ggagaccggg gcgccggcgg accgctacgg gttcgggccc ggcgcggaca
     3601 agctggagat cgggctccga ggcgcggcgg ggttgggcaa tacgctgtac ttcggtcagg
     3661 aggccggtcc gtcgggccgg tacgcgatga tccaggggca ggaaggcatt ttcgagttga
     3721 accgggagga ctgcgagcgg ctgctgccgc gcctgctgtc gaccgtcccg gtttccggga
     3781 gcgccgagaa tggagcccat gctgcagacg ccgccggcag ccccgccgtc ccctgacgcg
     3841 cgcgcaagcc gaaaacggcc cgcctttctc ttcagggcgg gccggcgcct cctccgattc
     3901 ctggcgggat tcgcgggact cgtcctcgtg gtgtctgccg cgctcctact gtactgcagc
     3961 gcggcgggct tccccgcctg gctcgttaag cgcgtgctgg cgcgattgga ggagcagggc
     4021 gtgcatgcgc gggtgcgccg ggcgcggttg agcgtcatgg acgggatcgt gctgaagggg
     4081 ttccgcctgt acgacgaccc ggcggccgct gcgcccgtga tcgaagccga tcgtttcgcc
     4141 ctgggcctga acgccctcga atggctgtcc cggcggcatg gcatcgtggg gcttcgcatc
     4201 cggggcgcca ggctccgcct ctacctggag gcccgcgacg acccgaacct ctcggagcag
     4261 gttgttctcg aaaacctcca cgccgccatc cggttccggc cgggcgcggt cgagatggag
     4321 cgcctctacg cggaggggct cggcatgcag ttccgggggg cgggcacgat ccatcggcgg
     4381 ttgccggaaa aggaaaccgg gcgatccggc gccgacctgt tgcgccgctg cccggactgg
     4441 ctcttcaagg cgctggaaca ggtccgggcg ctgcagttca gccagcctcc gcaggtccgc
     4501 ctggatttcg cgctggaact ggatgacccg gcggagtcgc gcgtggagtt gaaaatccag
     4561 tcgcgccgga cgcagacgcc ggtcgggctc ttcgatcgct ggagcgtgga cgcggccctg
     4621 gacggcagcc ggatgatgct caaatcgctg atcctgcggc agggggccgc ccggttgaac
     4681 ctgaatggcg ccctgtcgct ggcgaatcgc aaggcggaat tccggctgtt cagcgatctt
     4741 ccattgaaac agtggatggc catcctgccc cgggcgtggc ggctccggct ggaacagatg
     4801 ggatacgagt tgaagggaga ggcctcgctc gaactctggg gcgggccggc gccgtggagc
     4861 gaaatcaagc agcacctcgg cggctggatc tcgctcgaga aggccgagtg cggcggggta
     4921 tgggtggaga aggccttcct gtccgtcagc cgggagaatg gcgtttttac gctggagaag
     4981 ctggacggcg tggtgggccg gggcgtgcgg cagggccccc tgaaagcggc ggccgttctc
     5041 gataccgcct cgggagcttt ccacggggag atggaaggcg ccttcaatcc ccacctgtgg
     5101 aagccgttgc tgctgaaaca gcagatggag atcctggact ggttccagtt cggggacgtt
     5161 cccccggtcg tgaagctcga gttttccggt gtcgtcggcg acctgcagcg ttttgccttc
     5221 caggggagca tgagcgccac ctcgttcgcc taccgcgggg tcggtttgac cgccggttcc
     5281 gccggcctgg ccttgcgcga cggtgtcatg aggttggacc cgctggacat cgcgcgcgag
     5341 gagggcgggg cgcacggctg gatcgaactc gactacaacc ggcaaacagc ggcctttcaa
     5401 atagacagca cggccgatcc ccatgccgtg gcccgcatga tcggcccggg gcccgggcgc
     5461 ttcctggagg attaccggtt tgaaggcccg gcccatgtca cggccagcgg cgtggtcagc
     5521 tacgtcgggt tctccccggt cgacgtggag gcctgggtcg aggggcgcgg cgtcggcctg
     5581 ggctgggcgc tctcggacga ggccgcgttc cgcgtgcggg cctcgaacca gcgcgtggaa
     5641 atcaccgaca tccgcgcgga ggcttacggc ggacggctga ccgggcgcat ggccttctac
     5701 cccctgaaca agccgaacga tctgcgctac gacgccgtgg gccagcttgc cgacacccgc
     5761 ttcgaagacc tgatggcggc gctgatccgg ctcacggaga atccctacaa gggccggctt
     5821 tcggccgact tccgcctgga aggacgggtg ggcgagggcc gcggccggac cgccctcggc
     5881 gaggggcgca tccgcatcag ggacggcgcg cttttccaga tcccgctgat gggcgggttg
     5941 tccgagtggc tgacgcgtct ctatcccggc ctcgggttcg cgtcgcagac ggacttccgc
     6001 tcggaatttc tcctccagga cggcgcgttt cacacggagg aggccctgct cgagggttcc
     6061 gtgttgagcc tcaaggcgaa gggcgactat cgcctggacc ggcgtatcga catgacggtc
     6121 caggtccagc ccctgcgcga cggcgtcgtg gcggaagccg tgcgcctggc cacctcgccc
     6181 ctcaccaagc tgctcgagtt caaactgtcc gggaccatcg cggatccgaa gtggcggccg
     6241 gtcaacctgc ccaaggaaat gtttctcatc ttcgactgat ccccgccggc aaattaaccc
     6301 atgacaaagc ggcaggctcc cgttattgtt gcgccgtttt gttccgggag tgaaccgtgt
     6361 ccgcatggat gcatagctgc tcgacgctgg ccctcatggc cttggccgtt ggcctcgtgg
     6421 tggcggggct gaacgtccct tgcctgctct ggccggggag ggtgcgcgcc gccgccctcg
     6481 cttttccccg aagccgcgga ccggcctggc tgctgacggc gctcgacctg ggctgggtcg
     6541 cctggatcgt gctgcacgcc cagctgggac gctttgaatg ggtcaagccg tacctgtatg
     6601 ccgcggcgcc gctgtccttt ttcctgatcg tcatcttcat ggacgagctg ctggccccgc
     6661 gggcgctcgg tggattgctg ctgctgctgg cgaacccggt gatcatcgcc gcccgttggc
     6721 atgagtccga ctggcggctg gtgatgatcg gcctggccta cgtatgggcc gtggcgggca
     6781 tcgtgctggt cttgagcccg ttctatttcc gccgcgtggc ggcctgggcg ctggcgacgg
     6841 atggccgttg tcggcagctc ggtctccttc gcctggcgtt cggggcggcc ctcgtcgcgc
     6901 tggcgctggc cgtctattga tcttccatga gccggccggc ccgattcctg gtcctgcgcg
     6961 gcggcgccat cggggatttc ctgctgaccc tgccggtttt ccaggccctt cgcgcgcgct
     7021 ggcccgatgc ctggatcgag gtcctgggct atccgcacgt ggccggactg gcctcggccg
     7081 cggggctcgt gaaccgggtg gattcccttg atcgcgccgg cgtggcgcgg cttttctcgg
     7141 tggcgccgga cttccccgag gagcaggtgc ggcacctgcg ctccttcgat ttcgtcctct
     7201 cctatctgca cgatccgaca gggctggtcc gggagaacct gaaaagcgcc ggcgcccggg
     7261 tcgtcctgca cggttcgccg atcgtcgagc gcgggcacgc ggtggagcac ctgatgaagc
     7321 cgctggagag cctggccatc tatgccggcg gggagcatcc atcgctggca ttgcccgggg
     7381 ttcaccgcga ggccggccgg cgctggttcc gggagcgggg cctccgggaa cccgttctgg
     7441 ccgtgcatcc cggcagcggc agcgcggcga aaaactggcc gctcgaccgt tttgtcgaag
     7501 ccgcccggcg catcggggcc gctcgccatc ttgattattt ttatgtgctg ggcgaggcgg
     7561 atcgcggatt gagcgatgcc cttcaccgga tggatggcct ccggcctgtc ctgaaggaag
     7621 cgcccctgct ggaggtggcc ggcgtcctgt ccggcggccg gctctatctc gggaacgact
     7681 ccggcatcac gcatctggct gcagccgtgg gggtgccgac ggtggcgctg ttcgggccgt
     7741 cggacgccgt gcgatggggc ccgcgcggcc cgctcgtgcg cgtagtccgc gctcccgact
     7801 ccgacctgaa cgagttggcg gtggacgtcg tcctccaagt catggacggg ctgttggcgt
     7861 aaacggcgcg gcgttgcttc t
//
LOCUS       DAOOTK010000127         7873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5759_length_7873_cov_4.982093, whole genome shotgun sequence.
ACCESSION   DAOOTK010000127 DAOOTK010000000
VERSION     DAOOTK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5759_length_7873_cov_4.982093"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK634_11960"
     CDS             complement(<1..283)
                     /locus_tag="PK634_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOW98493.1"
                     /translation="MNLLHVCASNKPIDQSTSKQLAAAFFVRLAESNKDINVTNMDLY
                     NNPPPFLGNDAYRCFWNPILQPGYIPTASEKNAAGYALAQTKVFEEADVL"
     gene            complement(321..1523)
                     /locus_tag="PK634_11965"
     CDS             complement(321..1523)
                     /locus_tag="PK634_11965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOW98494.1"
                     /translation="MNRDPLHDQTTEAEDLTAFDPAGADLTTLADTPAEPAGKGSQQL
                     TANYKAILRARAIYYPVAYRFLKELGRGRQGVVFLGLRQGARGCVTRHAIKIFDPGIY
                     PSAKKYWTDMGRIAAQTSKLQLVKSPNLVAPEIYEESNGIGYIQMEVVDGVSLRYLLD
                     GKHLELVKPFCTPAEWARFTDVIFRVQGDRVSIQPGIALYIMRQALRGLETLHEMGFV
                     HSDIKPANIMIDRLGYVKLVDYGRAVRLNEKVSLLLGTPLYMAPEMHRREPSIAQGDI
                     YSVGAVGLEMLRGQPLLNPAGKSEPDLLNAKIEAVKKLPELLPDHVRQNAQFVVLLQR
                     FLDPEPKRRYPNAEEAEVGSQGLVLVHKQLAQLGKDTEYGRELENYLAKLMNPLPEPA
                     DLPAAGRE"
     gene            1690..2256
                     /gene="efp"
                     /locus_tag="PK634_11970"
     CDS             1690..2256
                     /gene="efp"
                     /locus_tag="PK634_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOW98495.1"
                     /translation="MYTVSDLRKGIKIELDGVPFEVVEFEFFKPGKGQAMYKCRIKNL
                     LTGAVTDRTFRAVDKIGQPDIEERDLVYSYPEGDHYVFMDHKTYEQVVISAEAVGDQK
                     YFLVEELEVSVLFFNGRAIQINLPSFVEKVIAETEPGARGDTATNVLKPAKLENGYEL
                     GVPLFINAGDTVRIDTRTGKYFDRVSKG"
     gene            2282..3208
                     /gene="epmA"
                     /locus_tag="PK634_11975"
     CDS             2282..3208
                     /gene="epmA"
                     /locus_tag="PK634_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOW98496.1"
                     /translation="MSADAEHLRAVRPALELRARLMRALRTFFADRGFLEIETPVRLR
                     APAPELHIDAEPSGDAWLRTSPELHMKRLLAAGYERIFQVGPCFRRGEFGARHRPEYA
                     MLEWYRAGADYHDVLVDTKALVLGLAREVLGTTRLRRGGREVTLEPAWAYLTVSEAFI
                     GFAGWDPVERFDPDRFDLDLVDKVEPQLPREVPVVLADYPAPAAALARRKPGRPAVAE
                     RWELYLDGIEIANAYSELTDPVEQAARFEEWNRARAAAGKPVYPPDREFLAALEKGLP
                     ACAGVALGVDRLAMLFAGAESLDEVLPFHEGA"
     gene            3254..5455
                     /locus_tag="PK634_11980"
     CDS             3254..5455
                     /locus_tag="PK634_11980"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOW98497.1"
                     /translation="MEAIVLLALVLLVLPIILASVAFVRTRSMAERLQALEARLARLA
                     GPPPAPRAEPVSAPTLPEPVLAAPRPALRRPVVPVPPVVAPPASSAKPSLELALGGKL
                     ASFIGIAALVTGVVFFVGYAIQREWIGPGLRVGLGLLAGLALAGLGYMAERRDRKYAV
                     LARALTGGGAALFYFSVFAAYQIYSLIPIPVALLGLLSSAGAALALAAFYDSQAVAVL
                     GVLGAFLTPPLIGSRLGVGLFPLVFAAVVNVPVLLLGLRRKWQWLYNLAFLFTVGLAA
                     AWLDRKLPGLADGAWRTGLGFVLLFHAEFVALGLLRLNTEPADEERPLDQLRLGLSTL
                     ALLGALRWIFGPAALRDWIGAAFALAALSHAGLARLGWIGRSGLRQEVMTLLLGAITF
                     ACFAVPVQFDGVWISLGWAVEGAALAGFAVWIRSDLLQGIAVFLGVLGLAKSVLFDVS
                     LYTVSPRVFLNGRFATGLLASGLLGVQGWLHGRSSSPQAPGRAQHLAALAVLAALVVF
                     FADVFHTMDTDLPWPWLFSTVAVFAAGWGLVLLAPRASLPGALGILLLLAVPVKILVD
                     VASCWDGYRAGFSPFLNGVIVAQLALLAGLCAARRLLARQARPADGRWPAGESINLLS
                     LASGILLVTLELHRSSFAWARPAITLWWAVCAMALAVAGILRRRAEWRYLALAVFCAA
                     VAKVYLVDLAVLKGLQRVAAFIGVGLLLLALSYLYQRLAPLWSPPGSADQR"
     gene            complement(5446..5886)
                     /locus_tag="PK634_11985"
     CDS             complement(5446..5886)
                     /locus_tag="PK634_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98498.1"
                     /translation="MKTILHPYGWALAALLLAGCATPESRIKKNPEMFDALAPEVQEQ
                     VRKGQIAVGFAEDAVFLALGKPDRMYTRQTESGTTEIWSYVGYYSTRDRQQVEYRGRV
                     RDAKGVSHSVSDTIWVDVDIRHDYERLRVEIKDKKVRAIERVQR"
     gene            5985..>7873
                     /locus_tag="PK634_11990"
     CDS             5985..>7873
                     /locus_tag="PK634_11990"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOW98499.1"
                     /translation="MKTEAWKLALALLLAAGPSSRAAFIGWASGGGDAQGGLLLCTTN
                     GTDWFRQGAGQFGTAELTGVAAVNGRHVWIAGEPENGYASIYYSADAGESWVRQGDAL
                     SLPDIRLAKLWAVDRDVLWVIGDAGTVLVTADGGDNWINVSLTDYTNMLQGVTAVDEY
                     TAWVSGDLDPDTGMAGLFYTEDGGATWTAQTNGAVTNAQHLLGLAALDADRVWAIGGP
                     EVMLATTNAGAEWALLRAGFPKDGNELRVLDDQRIWAAFDSHVDWTSNGGATWSNHNT
                     PDYTMDVSTPDGTSVWAVSGAYWGGQIYHSPDGGATWTNQAESNAWTRLFKVVVQPRP
                     EPRTLHVDAASTNAVAPFDSWDTAASNIQDAVEAAVEGDLVLVADGVYDQGGGYSTAG
                     KILNRVMINKTITVRSANGPAGAFIVGEGPDGPGAVRGAYLSEGACLYGFTVTDGHVF
                     LASSGLGADLYGGGAYLDGPATVSNCVIAGNSGGGAFLVQSREVSALGVPTLCHSTVQ
                     SNQARAGGGLSLEGGGVVRHCRIVENSSPGNGGGVMMAGNWPGAPCRLLNCFIAGNRA
                     TDEDGVLGGGVNAFGFFSVRHCTIVGNDATGDAGGLYAYGDPGGGSEVVNTILYHNTA
                     PTNPNL"
BASE COUNT         1068 a         2576 c         2842 g         1387 t
ORIGIN      
        1 cgagcacgtc ggcttcctcg aagaccttgg tctgggcgag ggcgtagcct gccgcgttct
       61 tctcggacgc ggtcgggatg tagccgggct ggaggatcgg attccagaag caccggtacg
      121 cgtcgttgcc caggaagggc ggcgggttgt tgtacaggtc catgttcgtg acgttgatgt
      181 ccttgttgga ctccgccagc cggacaaaga aagccgccgc gagctgtttg gaggtggatt
      241 ggtcgatcgg cttgttgctg gcgcagacgt gcagcaggtt catgtcttct cctatctatg
      301 ttcggttcgg caaaacgctt tcattcccgc ccggccgccg ggaggtccgc cggttccggc
      361 agcgggttca tcagcttggc caggtaattt tccaattccc gcccatattc cgtatctttg
      421 cccaactggg caagctgttt gtgcacgagc acgaggccct ggctgccgac ctcggcctcc
      481 tcggcgttgg gatagcggcg cttcggctcc ggatcgagga agcgctggag caggacgacg
      541 aactgggcgt tctgccggac gtggtcgggc agcagttcgg gcagtttctt cacggcctcg
      601 atcttcgcgt tgagcaggtc gggctcgctc ttgccggccg gattgagcag cggctggccg
      661 cgcagcatct cgaggccgac ggcgccgacg ctgtagatgt cgccctgggc gatgctcggc
      721 tcgcgccggt gcatctcggg cgccatgtag agcggcgtgc cgagcaggag ggatactttt
      781 tcgttcaaac ggaccgcgcg gccgtagtcc acgagcttca cgtaacccag ccggtcgatc
      841 atgatgttgg ccggcttgat gtcgctgtgg acgaagccca tctcgtggag cgtctccagc
      901 ccgcgcaggg cctggcgcat gatgtagagc gcgatgcccg gctggatcga gacccggtcg
      961 ccctgcaccc ggaagatcac gtcggtgaac cgcgcccact cggccggagt gcagaacggc
     1021 ttgaccagct ccaagtgctt cccgtcgagg aggtagcgaa ggctgacgcc gtcgacgacc
     1081 tccatctgga tgtacccgat gccgttgctt tcctcgtaga tttccggcgc gacgaggttc
     1141 gggctcttga cgagctggag cttcgaggtc tgggcggcga tacggcccat gtccgtccag
     1201 tacttcttgg cgctgggata gatgccggga tcgaagatct tgatggcgtg ccgggtcacg
     1261 cagccccgcg cgccctgccg gaggccgagg aagaccacgc cctgccggcc gcggccgagt
     1321 tccttgagga agcgatacgc gacgggatag tagatggccc gggcacgcag gatggccttg
     1381 tagttggccg tcagctgctg gctgcccttg ccggcgggct cggccggcgt gtccgccagc
     1441 gtggtcaggt ccgccccggc cgggtcgaag gccgtcagat cctccgcctc ggtcgtctgg
     1501 tcgtgcaagg ggtcgcgatt cacgcgccac agtttacgcg ggaatgtcga ataggcaaca
     1561 gcagagtgcg gagcgggccg cggatgaaaa aacgcttggc aatccgggcg gtccccggca
     1621 tagtgcatgc ccggtttcgt cccggccacg gggcgcggtg tggcggttca tcaagcggaa
     1681 ggtgtggaca tgtatacggt ttcggatctt cgcaaaggca tcaagatcga gctcgacggc
     1741 gtgccgttcg aggtcgtgga gttcgagttc ttcaagcccg gcaagggcca ggcgatgtac
     1801 aagtgccgca tcaagaacct gttgacgggc gccgtcacgg accggacctt ccgcgccgtg
     1861 gacaagatcg gccagccgga catcgaggag cgggacctcg tctattccta ccccgagggc
     1921 gatcactacg tcttcatgga tcacaagacc tacgagcagg tcgtcatcag cgcggaggcc
     1981 gtgggcgacc agaagtattt cctcgtcgag gagctggagg tcagcgtgct gttcttcaac
     2041 gggcgcgcca tccagatcaa cctgccgtcc ttcgtggaga aggtcatcgc cgagaccgag
     2101 cccggcgcgc gcggcgacac cgcgaccaac gtgctcaagc cggccaagtt ggaaaacggc
     2161 tacgagctgg gcgtgccgct cttcatcaac gccggcgaca ccgtccgcat cgacacccgg
     2221 accggcaagt acttcgaccg cgtgagcaag ggctgacggg ctcgcggccc gcggggctcc
     2281 catgagcgcc gacgccgaac acctccgcgc cgtccgcccc gcgctggaac tgcgcgcgcg
     2341 cctgatgcgg gccctgcgca ccttcttcgc cgaccgcggc ttcctggaga tcgagacgcc
     2401 cgtccggctg cgggcccccg cgccggaact ccacatcgac gccgagccct cgggcgacgc
     2461 ctggctgcgc acctcgcccg agctccacat gaagcggctg ctcgccgccg gctacgagcg
     2521 gatcttccag gtcggcccct gcttccgccg gggcgagttc ggagcccggc accggccgga
     2581 gtacgccatg ctggaatggt accgcgccgg cgccgactac cacgatgtcc tggtcgacac
     2641 gaaagcgctc gttcttggcc tggcgcgcga ggtcctgggc acgacgcggc tccgccgcgg
     2701 cggccgcgaa gtgaccctcg agcccgcgtg ggcatacctg accgtctccg aggcgttcat
     2761 cgggttcgcg ggctgggatc ccgtcgagcg gttcgatccc gaccgcttcg atctcgacct
     2821 ggtggacaag gtcgaaccgc aactgccgcg cgaggtgccg gttgtcctgg ccgattaccc
     2881 cgcgccggcc gccgccctcg cgcggcgcaa accgggccgg cccgccgtgg ccgagcggtg
     2941 ggagctgtac ctggacggga tcgagatcgc caacgcctac tccgagctga ccgatcccgt
     3001 cgagcaggcc gcgcggttcg aggaatggaa ccgcgcgcgg gccgccgccg gcaagccggt
     3061 ctacccgccg gaccgggaat tcctggcggc gctggaaaaa ggactgcccg cgtgcgcggg
     3121 agtggcgctt ggcgtggatc gcctggccat gctcttcgcc ggcgcggaat cgctggacga
     3181 ggtgctgccg tttcacgagg gggcttgatc gctccggccg ggacgcggta gcattccggt
     3241 ggggaggtcg ccgatggaag cgattgtttt gctcgcgctc gtgctgctgg tgctgccgat
     3301 catcctggcc tctgtcgcct ttgtcagaac ccgatccatg gccgagcggc tgcaggcgct
     3361 ggaggcccgg ctggcgaggc tggccggccc gccgcctgcg ccccgggccg aacccgtgtc
     3421 ggcgccgacg ctgcctgaac ctgtcctcgc ggcgccgcgg ccggctctgc gacggcccgt
     3481 tgtacctgtc cccccagtgg tcgcgccgcc ggcgtcgtcc gcgaagccga gtctggaact
     3541 ggcgctcggc gggaaactgg ccagcttcat cgggatagcc gccctggtga cgggcgtggt
     3601 cttcttcgtg ggctacgcga ttcagcgcga gtggatcggg cccgggctcc gggtcggcct
     3661 gggcctgctc gcgggcctgg cgctggccgg tctgggatac atggcggagc ggcgcgatcg
     3721 gaaatacgcc gtgctggcgc gcgcgctgac cggcggcggg gcggcgttgt tctacttcag
     3781 cgtgtttgcc gcgtatcaga tttactcgct cattccgatc cccgtcgccc tcctgggctt
     3841 gctgtccagc gcgggcgccg cgctggccct ggcggcgttc tacgacagcc aggccgtggc
     3901 cgtgctcgga gtgctgggcg cgtttctcac gcccccgctg atcggcagcc gcctgggcgt
     3961 gggcctcttt cccctggtat ttgcggccgt ggtcaatgtc cccgtcctgc tgctgggcct
     4021 gcgccggaaa tggcagtggc tgtataacct ggccttcctg ttcaccgtgg ggttggccgc
     4081 cgcctggctc gatcgcaagt tgccgggcct ggcggacggc gcctggcgga cgggcctggg
     4141 ctttgtcctg ctgtttcacg cggagtttgt ggccctgggc ctgctccgcc tgaacacgga
     4201 gccggccgac gaggagcggc cgctcgacca gcttcgcctc gggttgtcca cgctggcgct
     4261 gctgggcgcg ttgcgctgga ttttcgggcc ggcggcgttg cgcgactgga tcggcgcggc
     4321 cttcgccctg gcggcgctgt cgcacgcggg gctggcgcgg ctcgggtgga tcgggcgatc
     4381 aggcctccgg caggaggtca tgaccctgct gctgggcgcg atcacctttg cctgctttgc
     4441 cgtgccggtc cagttcgacg gcgtatggat ttcgctgggc tgggcggtcg aaggcgccgc
     4501 cctcgccggg ttcgccgtgt ggatccggtc ggacctcctg caagggatcg ccgttttcct
     4561 cggcgtgctg ggcctggcca agtccgtcct gttcgacgtc tcgctgtata ccgtctcgcc
     4621 gcgcgttttc ctgaacgggc ggttcgcgac cgggctgctg gcctcggggt tgctgggcgt
     4681 gcagggctgg ctgcacgggc gttcctcctc gccgcaggcc ccgggccggg cgcagcacct
     4741 ggccgccctg gccgtgctgg ccgcgctggt cgtgttcttc gccgacgtgt tccatacgat
     4801 ggacacggat ctcccctggc cgtggctgtt cagcaccgtg gcggtttttg ccgcgggctg
     4861 ggggctggtg ctgctcgcgc cgcgcgcgtc gctgccgggc gcgctcggga tcctcctgct
     4921 gctggccgtg ccggtgaaga tcctggtgga tgtcgcgagc tgttgggatg gctaccgcgc
     4981 cggcttctcc cccttcctca acggcgtgat cgtggcgcaa ttggccctgc tggccggcct
     5041 ctgcgcggcg aggcggctgc tggcacgcca ggccaggccc gccgacgggc gttggccggc
     5101 gggcgagtcg atcaacctgc tgtcgctggc gtccgggatc ctgctggtga cgctggaact
     5161 gcaccgctcg tccttcgcct gggcgcggcc ggccatcacg ctctggtggg cggtgtgcgc
     5221 gatggcgctg gcggtggcgg gcatcctgcg gcgccgggcg gagtggcggt acctggccct
     5281 ggccgtgttc tgcgcggccg tggccaaggt gtacctggtg gacctggccg tgctgaaagg
     5341 cctccaacgc gtggccgcct ttatcggcgt gggcctgctg ctgctggcgc tgtcctatct
     5401 ctatcagcgc ctcgcgccgc tctggagccc gcccggttcg gcggatcagc gctgaacccg
     5461 ctcgatggcg cggaccttct tgtccttgat ctccacgcgc agcctctcgt agtcgtgccg
     5521 gatatccacg tccacccaga tcgtgtccga gaccgaatgc gagacgccct tcgcatcgcg
     5581 gacgcggccc cggtattcca cctgctggcg gtcccgggtc gagtagtacc cgacgtacga
     5641 ccagatttcg gtcgtgccgg attccgtctg ccgggtgtac atgcggtccg gcttgccgag
     5701 ggcgaggaag acggcgtcct cggcgaagcc gacggcgatc tggcccttgc gcacctgctc
     5761 ctggacctcg ggggccaggg cgtcgaacat ctccgggttc ttcttgatgc ggctctcggg
     5821 cgtcgcgcag cccgcgagca gcagggccgc gagggcccag ccgtacggat ggagaatcgt
     5881 cttcatgttt acatcttaca tccccggacc cgttcgataa agtctctttc ggacttcagc
     5941 cgcggcggcc gatccggtat gctggcccct gtcaaggagg cgagatgaaa accgaggcct
     6001 ggaaactggc tctggccctg ctcctggcgg ccggtccgtc gtcgcgcgcg gcgtttatcg
     6061 gctgggcgag cggcgggggc gacgcgcagg ggggattgct gctgtgcacg acgaacggga
     6121 ccgactggtt ccggcagggc gccgggcagt tcggcacggc ggagctgacg ggcgtggccg
     6181 cggtcaacgg gcggcacgtc tggatcgcgg gcgagccgga aaacggctat gcctcgatct
     6241 actactcggc ggacgccggc gaaagctggg tccggcaggg cgacgccctt tcgctgccgg
     6301 atatccggct ggccaagctc tgggcggtgg accgggatgt cctctgggtg atcggcgacg
     6361 cgggcacggt gctggtgacc gcggacggcg gggataactg gatcaacgtt tcgttgacgg
     6421 attacacgaa catgctgcag ggcgtgacgg ccgtggacga gtacacggcg tgggtcagcg
     6481 gcgacctgga tccggacacg gggatggccg gtctctttta taccgaggac gggggcgcga
     6541 cctggacggc gcagacgaac ggcgccgtga cgaacgcgca gcacctgctg ggattggcgg
     6601 cgctggatgc cgaccgcgtg tgggcgatcg gcgggcccga ggtcatgctg gccaccacca
     6661 acgcgggcgc ggagtgggcc ctgctccgcg cgggattccc caaggacggc aacgagcttc
     6721 gcgtgctgga cgaccagcgc atctgggcgg cgttcgactc gcacgtggac tggacgagca
     6781 acggcggcgc gacctggagc aaccacaaca cgccggacta cacgatggac gtctccacgc
     6841 cggacgggac cagtgtctgg gcggtgtccg gcgcctattg gggcgggcag atctaccatt
     6901 ctcccgacgg cggcgcgacg tggacgaacc aggcggaatc caacgcgtgg acgcgccttt
     6961 tcaaggtcgt ggtacagccc cggcccgagc cgcgcacgct ccacgtggac gcggcctcga
     7021 ccaacgccgt ggcgccgttc gattcctggg acacggcggc ctcgaacatc caggacgccg
     7081 tggaggccgc cgtggagggc gacctggtgc tcgtcgcgga cggcgtgtac gaccaggggg
     7141 gcggctattc gaccgccggc aagatcctga accgggtgat gatcaacaaa acgatcaccg
     7201 tgcgcagcgc gaacggcccg gcgggcgcgt tcatcgtcgg cgaggggccg gacgggccgg
     7261 gcgccgtgcg cggggcctat ctctcggaag gcgcctgcct gtacggcttc acggtcaccg
     7321 acgggcatgt tttccttgcg tcgtcgggcc tgggcgccga cctgtacggc ggcggggcgt
     7381 acctggacgg gccggccacg gtcagcaact gcgtgattgc cggcaacagc ggcggcgggg
     7441 cgttcctggt tcagagccgg gaagtatcgg cgctcggcgt cccgacgctg tgccactcga
     7501 ccgtgcagag caaccaggcg cgggccggtg gcggcctctc gctcgaaggc ggcggagtgg
     7561 tccgccattg ccggatcgtg gagaacagtt cgccgggcaa cggcgggggc gtgatgatgg
     7621 cggggaactg gccgggcgcg ccgtgccggc tgctcaactg cttcatcgcc gggaaccgcg
     7681 cgacggacga ggacggcgtg ctgggcggcg gcgtcaacgc cttcggattt ttctcggtcc
     7741 gccattgcac gatcgtcggg aacgacgcga ccggcgacgc ggggggattg tatgcctacg
     7801 gggatccggg aggaggttcg gaggtcgtca acacgatcct ctaccacaac accgcgccga
     7861 cgaacccgaa cct
//
LOCUS       DAOOTK010000128         7858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5780_length_7858_cov_3.505831, whole genome shotgun sequence.
ACCESSION   DAOOTK010000128 DAOOTK010000000
VERSION     DAOOTK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_7858_cov_3.505831"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK634_11995"
     CDS             complement(<1..488)
                     /locus_tag="PK634_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOW98500.1"
                     /translation="MSWFLKQSDGQTYGPHEEDTLRQWAADGRIGADDQISQDQQHWY
                     PAPDLVSLEMDWMIRLPDGSLFGPVHLKAIPLLAQSGEVSADAEIVNRRTAQVMSVQA
                     ALVGGAAESAPPPQATWKDLAQSRDFHDHEARKWKNLYEQERDRAAQTETGLSRKLAE
                     MR"
     gene            complement(514..1077)
                     /locus_tag="PK634_12000"
     CDS             complement(514..1077)
                     /locus_tag="PK634_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOW98501.1"
                     /translation="MKQAWIVAAAGLSLAGCVTYDDAQREAAAGHEDYLLVQEQMRRL
                     DGRLEGVELEISRLQTNLETLRAGQSRADTSETQAIQARVEDLDRRLRALDAARQQDK
                     QEIVDSLSKKIAQLVGSASSGSSAKKQSGSRKSGPSTGYEHEVQPGESLSAIAAAYGV
                     SSKVIMEDNGITDPDKLRVGQKLFIRD"
     gene            complement(1610..2809)
                     /locus_tag="PK634_12005"
     CDS             complement(1610..2809)
                     /locus_tag="PK634_12005"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOW98502.1"
                     /translation="MKKRKIVVLVGDGMGDYPVPELGGRTPLQAARIPHIRRLAAAGD
                     LRMVQTVPVAGLSPGSDVANLGLLGYKPEDHYTGRAPIEAAGAGIPLAADDVAFRCNL
                     VTVADGRMDDYSAGHITTEEGRQLVASVEKAMGRPGLCFHGGVSYRHLLVWKGGPAGI
                     RTQPPHDIAGQPVADHLPAGEQQDAVRALMEQSIGLFRDHPVNRARIAAGKKPATQIW
                     LWGQGLSLKLPTYPELYGLTGGVITAVDLIRGLGRLIGLDVIQVPGATGFLDTNYAGK
                     VQAALDVVARHDFVFVHIEAPDECGHMGKVALKVEAIEAFDQKVVAPVWEALEKKGEP
                     YRLLLCTDHRTPVSVRGHTREPVPMISLDGPVGAIDREAAFDEFVGGGKASGMIHERM
                     RELLRTA"
     gene            2918..3904
                     /locus_tag="PK634_12010"
     CDS             2918..3904
                     /locus_tag="PK634_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOW98503.1"
                     /translation="MSERLYPFQFKPVYKDYLWGGDRIIRRYGRREPPGVYAESWEIS
                     DHPDGMSVVVNGPLAGLALHELVQQFGQALLGRDDPRTTFPLIIKILDARERLSVQVH
                     PGEETAPHQGGDPKTEMWYVLEAEAGAGVFAGLKPGVSRADLLRALEAKTLEEKLRFV
                     PVSAGQVVFVPAGRVHAIDAGCLLFEVQQRSNTTYRLYDWDRAGPDGRPRDLHIEQAL
                     RAVRWHDDLPVAVSEGAPRHIGGNERRTVMDTPYFLVDHWRLAAPRLLPAKRTGLTFQ
                     ALFVIAGVLRVEGGGQTVSLAAGSSALLPAVLEGGRWIPEQAGTEILCVTRP"
     gene            complement(3932..4912)
                     /gene="corA"
                     /locus_tag="PK634_12015"
     CDS             complement(3932..4912)
                     /gene="corA"
                     /locus_tag="PK634_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOW98504.1"
                     /translation="MIRSFIFNQGKLVSQDVGLDVLRLLLYDEGVQIWADAEKPTEAE
                     YKGLLEGVFNFHPLAIEDCIAVTERPKIDEYDQYLFMVVHAADYRHETHRFQTTELNM
                     FIGRNFLVTVHEDPLKSVASTIDRVLKNAPVIARAPDRLTYHLLDALLENYEPALLDL
                     SSELGELEKRALTHTSVSILTDVLQLKSEVQRLRQVVTPQRDVLSRMAHGEFKIVRAA
                     MLPYYRDLMDHLSRISDRIDTYRDTLTNALQINLNIQQMQVNRVIKVLTVMATLAAPL
                     LIVTSFYGMNIQHMPNTDWPSWPLAYGMILGGTTIMTVLLYWFLRRRGWS"
     gene            complement(4970..5941)
                     /locus_tag="PK634_12020"
     CDS             complement(4970..5941)
                     /locus_tag="PK634_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOW98505.1"
                     /translation="MNLLGIDLGGTKTSVCIGDENGTIRAARRIPTLASEGPESGLRR
                     TAGLARALLDETGLDPARVDAVGISAPGPVSVREGLMLRPPNMPGWVDVPLVRWAQDS
                     FGRPVFMNNDANACALAEYRYGSCRGTPNLVYLTMSTGLGAGIVLDGNLVQGATDLAG
                     EVGHHVLDIRGPPCPCGQRGCLEIYCGGKNVADRLRARIVKDNARSAILGEAGGDPAA
                     IDFRAFLAAVKKDDALALEFWEEYLERLAQGVGTVIMFFNPQVILMGTIAIHAGEFLL
                     APLRERVRRYAWAPSVAACRILPSALEGRIGDLSALAVAADGLPPRV"
     gene            6278..6952
                     /locus_tag="PK634_12025"
     CDS             6278..6952
                     /locus_tag="PK634_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOW98506.1"
                     /translation="MSGKLIVIILIVASVFAWSVMVTKYLDLTRAKRASRRFQLAFRN
                     EAHPLSLYLKRHAFAESPIYKVYEAGCHALGGELNDGLHAGADLFSAGSRLDDYRLNH
                     LQVEVVRRVTERTVADQALEMESKMGFLATAVSASPLLGLLGTVWGVLDAFGAMAVSG
                     AANLSAVAPGISAALLTTVVGLLVALPSSIGYNILASQIREIAVQMDNFAQEFVAELQ
                     RMFVRE"
     gene            6965..7375
                     /locus_tag="PK634_12030"
     CDS             6965..7375
                     /locus_tag="PK634_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOW98507.1"
                     /translation="MARRTSILALQEMREINMTPLIDLTFLLLITFIITFPLLEQGIY
                     VNLPRGKADDLDQRQMRTITLDIEGQLFLDRQPVTRDALAAEMTEMGRADPETIVMVR
                     ADEKIAYGKLVEILNILREAKIARMALVTSPDGK"
     gene            7382..>7858
                     /locus_tag="PK634_12035"
     CDS             7382..>7858
                     /locus_tag="PK634_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98508.1"
                     /translation="MDRRFRKSLKISSIAHVLLILGVILAPLLANWYLRRQRRDIVVI
                     DLTIALPDIPAEAVAEAAAAPPAPEPPKDIPEEIKPRRQVQKSTKRVTREPPKPDKPK
                     LTPEEIRKLLAAGAKISDRTSVPTGTFPEAWYYALVKQTMYDAWNQPSGPAVSAGLR"
BASE COUNT         1212 a         2649 c         2702 g         1295 t
ORIGIN      
        1 tcgcgcatct cggccagttt ccgggagagc cccgtttccg tctgggccgc gcggtcccgt
       61 tcctgctcgt acaggttttt ccacttgcgc gcctcgtggt cgtggaaatc ccggctctgg
      121 gccagatcct tccaggtggc ctgcggcggc ggcgcggact cggcggcccc gccgaccagc
      181 gccgcctgca cgctcatgac ctgggcggtc cggcggttca cgatctcggc atcggcagag
      241 acctccccgg actgcgccag cagggggatc gccttcaggt ggacgggccc gaacagcgag
      301 ccatccggca agcgtatcat ccagtccatc tccagcgaga cgaggtcggg ggcgggatac
      361 cagtgttgct ggtcctgcga gatctggtcg tccgccccga tgcggccgtc ggccgcccac
      421 tgccggagcg tatcctcctc gtgcggcccg tacgtctgcc cgtcggattg tttcaggaac
      481 caggacatcg cgtggtttcg gggtgtcggg acatcagtcg cggatgaaaa gtttctgccc
      541 gacccgcagc ttgtcggggt ccgtgatgcc gttgtcttcc atgatgacct tggagctgac
      601 gccgtacgcc gcggcgatgg ccgacagcga ctcgcccggc tggacctcgt gctcgtagcc
      661 cgtggacggt cccgacttgc gggatccgct ctgcttcttc gcggaggagc cggacgaggc
      721 ggagccgacc agctgcgcga tcttcttgct caagctgtcc acgatctcct gcttgtcctg
      781 ctgacgcgcg gcatcgaggg cgcgaagccg ccggtccagg tcctccacgc gggcctggat
      841 ggcctgggtc tccgaggtgt cggcgcggga ctggccggcg cggagcgtct ccaggttggt
      901 ctggagccgc gagatctcca gttccacgcc ctccagccgc ccgtccagcc ggcgcatctg
      961 ttcctgcacg agcaggtaat cctcatggcc ggcggccgct tcccgttgcg cgtcgtcgta
     1021 ggtcacgcag ccggccaggg acagcccggc cgccgcgacg atccaagcct gtttcatctt
     1081 tgcatcctca ccgtttcatt tctacgtact cttcgcaggc cgccttctca agcaaaatgg
     1141 aatccgcggc atcggggaag gggccggctg cgtgctcgcg cggcccgcag ccttgcgcca
     1201 gtccatacaa taagtataac aattgcaatc acggatgtga atagaaatgt tataatacaa
     1261 actacccatt ccgctgcgcg cgggcgtggg aaaggacagg ccggaaccgc gccggcctgc
     1321 tccgtaatca tctccgcgca cgacgagggg cgttggtccg gcgaggattt cgacttcgcg
     1381 gtgcgcctga aaaaagcatg gccgggccac gcggcggcgg ttcgacatcc tgcggaaagc
     1441 cccgctggtc acgtcctgcc gcacgttcga ccggtggggc gactggttcg cgtgcaaacg
     1501 gctgctccgc catccgcggg cgttcttcga cgcctgccgc ggccgggacc cggaacgggc
     1561 ccgtcgtttc cggtacgagg cggagcggtt ccggaaaccg tgagccgcgt caggccgtgc
     1621 gcagcagttc gcgcatccgc tcgtggatca tgccggaggc cttgccgccg ccaacgaact
     1681 cgtcgaaagc cgcctcgcgg tcgatggcgc cgaccggccc gtccaggctg atcatcggga
     1741 cgggttcccg ggtgtggccg cgcacgctga cgggggtgcg atgatcggtg cagagcagca
     1801 ggcggtaggg ttcgcccttt ttctccaggg cctcccagac cggcgcgacc accttctggt
     1861 cgaaggcttc gatggcctcg accttcaagg cgaccttgcc catgtgtccg cactcgtcgg
     1921 gcgcctcgat gtgcacgaag acaaaatcgt gccgggcgac cacgtccagc gccgcctgca
     1981 ccttgcccgc gtagttggtg tcgaggaacc ccgtggcccc cgggacctgg atgacgtcga
     2041 gcccgatcag gcggccgagg ccgcggatca ggtccacggc ggtgatcacg ccgccggtga
     2101 gcccgtatag ctcggggtag gtgggcagct tgagggagag gccctggccc cagagccaga
     2161 tctgggtcgc cggcttcttg cccgcggcga tccgggcgcg gttgacgggg tgatcgcgaa
     2221 agagcccgat ggactgctcc atcagcgcgc gcaccgcgtc ctgctgctcg ccggccggaa
     2281 ggtgatcggc gacgggctgg ccggcgatgt cgtggggcgg ctgggtcctg atccccgccg
     2341 gcccgccctt ccagacgagc aggtgccggt agctgacccc gccgtgaaaa caaaggccgg
     2401 gccggcccat ggccttctcc acggaggcga cgagctggcg gccttcctcg gtcgtgatgt
     2461 ggccggccga gtaatcgtcc atgcggccgt ccgcgacggt cacgagattg cagcggaacg
     2521 ccacgtcatc ggccgccaga gggatgccgg cgccggcggc ctcgatgggc gcgcggccgg
     2581 tgtaatgatc ctcgggcttg taccccagca ggcccaggtt ggccacgtcg ctgcccgggg
     2641 agagcccggc cacgggcacg gtctgcacca tccgcaggtc gccggcggcg gccaggcggc
     2701 ggatgtgcgg tatgcgggcg gcctgcaggg gagtgcgccc gcccaattcc gggaccgggt
     2761 agtctcccat gccgtccccg accagcacga cgatttttcg ctttttcata tggagccgat
     2821 aacctaccgt aagcggacgg ctgggtcaaa ttcggcttgc ccggccggcc cggccgggaa
     2881 atacttcccg gcctgtcggc gggaaagggc ggggatcgtg tcggagcggc tttatccatt
     2941 tcaattcaag cctgtctaca aggattactt gtgggggggc gaccgcatca tccgccggta
     3001 cgggcgccgc gagccgccgg gcgtgtatgc cgagtcctgg gagatctccg atcaccccga
     3061 cggcatgagc gtcgtcgtca acgggcccct ggcgggcctg gcgttgcacg agctggtgca
     3121 gcagttcggc caggccctgc tcgggcggga cgatccccgc acgacctttc cgctgatcat
     3181 caagatcctg gacgcgcgcg agcggttgag cgtgcaggtg catcccgggg aggagacggc
     3241 gccgcaccag ggcggcgatc ccaagacgga gatgtggtac gtcctcgagg cggaggccgg
     3301 cgcgggcgtg ttcgccggcc tgaagcccgg cgtgagccgg gccgatctcc tccgcgcgct
     3361 ggaggccaag accctcgagg agaagctccg ttttgtcccc gtgtccgccg gccaggtggt
     3421 tttcgtgccg gccgggcgcg tgcacgcgat cgacgcgggc tgcctcctgt tcgaagtcca
     3481 gcagcgctcg aacacgacat accgcctgta cgactgggac cgggccgggc cggacgggcg
     3541 cccccgggac ctgcacatcg aacaggccct gcgcgcggtc cggtggcacg atgatctgcc
     3601 ggtggcggtg tcggaaggcg ccccgcgcca tatcggcggc aacgagcgcc gcacggtgat
     3661 ggacacgccc tacttcctcg tcgatcactg gcggctggct gcgccccgcc tcctgcccgc
     3721 gaagagaacg ggactgacgt tccaggccct cttcgtgatc gccggggtcc tgcgcgtgga
     3781 agggggcggg cagacggtat cgctggcggc ggggtcctcg gcccttctgc ccgccgtcct
     3841 cgagggcggc cggtggatac cggaacaggc ggggacggaa atcctctgcg tcacgcggcc
     3901 ctgaagggaa gaacaagccc gcgccggccg gtcaagacca gccgcgccgc cgcaggaacc
     3961 agtagagcag caccgtcatg atggtcgtcc cgcccaggat catcccgtag gccagcggcc
     4021 agctcggcca atccgtgttg ggcatgtgct ggatgttcat cccgtagaag ctggtgacga
     4081 tcagcagggg cgcggcgagc gtcgccatca cggtgagaac cttgatgacc cggttcacct
     4141 gcatctgctg gatgttgagg ttgatctgca gcgcgttggt cagcgtgtcg cggtacgtgt
     4201 cgattcgatc gctgatccgg gacaggtgat ccattaagtc gcggtagtac ggcagcatgg
     4261 ccgcgcgcac gatcttgaat tccccgtggg ccatccgcga aagcacgtcg cgctgcgggg
     4321 tcacgacctg ccggaggcgt tggacctcgg atttcaactg gagcacgtcc gtgaggatgc
     4381 tcaccgaggt gtgggtcagc gcccgttttt ccagctcgcc gagctcgctg gacaggtcca
     4441 gcagcgcggg ctcgtaattc tccagcagcg cgtcgagcag gtggtaggtc aggcggtccg
     4501 gcgcgcgggc gataaccggc gcgttcttga gcacgcggtc gatggtgctg gcgacgctct
     4561 tcaggggatc ctcgtgcacc gtcacgagga agttccggcc gatgaacatg ttgagctcgg
     4621 tcgtctggaa ccggtgggtc tcgtgccggt agtccgccgc gtggacgacc atgaacaggt
     4681 actggtcgta ctcgtcgatc ttggggcgct cggtgacggc gatgcagtcc tcgatggcca
     4741 gcgggtggaa gttgaagacg ccctcgagca ggcccttgta ctcggcctcg gtcggtttct
     4801 ccgcgtccgc ccagatctgc acgccctcgt cgtagagcag cagccgcagc acgtcgagcc
     4861 cgacgtcctg gctgacgagc ttgccctggt tgaatatgaa cgatcgaatc atgagacggc
     4921 ggccccgtat ccgcgcgcat cctctcatgc gctccggggg gactcaaggt caaacgcgcg
     4981 gcggcaggcc gtcggcggcc acggcgaggg cgctcaagtc gccgatccgt ccctcgaggg
     5041 cgctgggcag gatccggcag gcggccacgc tgggcgccca ggcgtagcgg cgcacgcgct
     5101 cgcggagcgg ggccagcagg aactcgccgg catggatggc gatggtgccc atcaggatca
     5161 cctggggatt gaagaacatg atcacggtgc ccacgccctg ggccagccgc tccaggtatt
     5221 cctcccagaa ctcgagggcg agggcgtcgt ccttcttcac ggcggcgaga aaggcgcgga
     5281 agtcgatcgc ggccgggtcg ccgcccgcct cgcccaggat cgccgagcgg gcgttgtcct
     5341 tcacgatgcg cgcccgcagc cggtcggcga cattcttccc gccgcagtaa atttccaggc
     5401 agccccgctg cccgcagggg cagggggggc cgcggatatc gaggacgtgg tgcccgactt
     5461 cgcccgcgag gtccgtggcg ccctggacca ggttcccgtc caggacgatc ccggcgccga
     5521 ggcccgtgct catggtcagg tagaccaggt tgggcgtgcc ccggcaggat ccgtagcggt
     5581 actcggccag cgcgcacgcg ttggcgtcgt tgttcatgaa cacgggccgc ccgaacgagt
     5641 cctgcgccca gcggacgagg ggcacgtcca cccagccggg catgttcggc gggcgcagca
     5701 tcaggccctc gcgcacggag acgggtcccg gcgccgagat gccgacggcg tcgacgcggg
     5761 ccgggtcgag gcccgtctcg tcgagcaggg cgcgcgccag tccggccgtc cgccggagcc
     5821 cgctttcggg tccctccgac gcgagggtgg ggatgcggcg ggcggcgcgg atggtcccgt
     5881 tctcgtcgcc gatgcacacc gacgtcttgg tgccgccgag gtcgatgccc agcaggttca
     5941 tggccgtgga gcatacggga tccgcggccg ggccgggagc acaatttgcg ggcggccgcg
     6001 gctctcggcc agggttcagc gctcccggca accccgctcc aaaatgtaac ataatacttc
     6061 acgggtgtga agagttatgt tacccccgga cgggatcggc ggcggaggag ggcggggccg
     6121 cgcggcccga tgtccattga cccgtgttcg acgcttcgat aaggtacgcg ccgagaattc
     6181 aaaccgttca agttataaag aggtgcacgt gcccatcgcc gctttaccgc cgcttcccgt
     6241 cgccagcatg ttctcggcgt atcagacgag cagcttcatg tccggcaagc tcatcgtcat
     6301 catcctgatc gtcgcctcgg tcttcgcctg gtccgtgatg gtgaccaagt acctggatct
     6361 cacccgcgcc aagcgggcct cgcgccggtt ccagctggcg ttccgcaacg aggcgcaccc
     6421 gctctcgctc tacctcaagc ggcacgcctt cgccgaaagc ccgatttaca aggtctacga
     6481 ggccgggtgc cacgccctgg gcggcgagtt gaacgacggg ctccacgccg gcgcggacct
     6541 gttctcggcc gggagccgcc tggatgacta ccgcttgaac cacctgcagg tggaggtcgt
     6601 gcgccgcgtc acggagcgca cggtggccga ccaggcgctg gagatggaga gcaagatggg
     6661 ctttctcgcc accgccgtca gcgccagccc cctcctgggc ctgctgggca ccgtctgggg
     6721 cgtgctggac gccttcggcg ccatggccgt cagcggcgcg gccaacctgt ccgccgtcgc
     6781 ccccggcatc tcggcggccc tgctgaccac ggtcgtcggc ctgctcgtcg cgctgccctc
     6841 gtccatcggg tacaacatcc tcgccagcca gatccgcgag atcgcggtgc agatggataa
     6901 tttcgcgcag gaattcgtgg ccgaactgca gcgcatgttc gtaagggaat aagggagtcc
     6961 gaccatggcc cgacgaacgt ccatcctggc gctgcaggaa atgcgggaga tcaacatgac
     7021 cccgctgatc gacctgacct tcctgctgct catcacgttc atcatcacct tcccgctgct
     7081 cgaacagggc atctacgtca acctgccgcg gggcaaggcg gacgacctcg accagcggca
     7141 gatgcggacc atcaccctcg acatcgaggg ccagttgttc ctggaccgcc agccggtgac
     7201 gcgcgacgcc ctcgcggcgg agatgaccga gatgggccgg gcggacccgg agaccatcgt
     7261 catggtccgg gccgacgaga agatcgctta tgggaagctc gtggagattc tcaacatcct
     7321 gcgcgaggcg aagatcgcgc gcatggcgct ggtgacttcg ccggacggga aataacgcct
     7381 catggaccgg cggttccgca agtcgctgaa gatcagttcc atcgcccacg tgctcctcat
     7441 cctcggcgtg atcctcgccc cgctgctcgc caactggtac ctccgacgcc agcggcggga
     7501 catcgtggtc atcgacctga ccatcgccct gccggatatc ccggcggagg ccgtcgccga
     7561 ggcggccgcc gcgccgcccg ccccggagcc gcccaaggac atcccggagg agatcaagcc
     7621 gcggcgccag gtgcagaaga gcacgaagcg cgtcacgcgc gagccgccca agccggacaa
     7681 gccgaagctc accccggagg agatccgcaa gctgctggcc gccggggcga agatatccga
     7741 ccgcacctcc gtcccgacgg gcacgttccc cgaggcgtgg tattacgcgc tcgtgaaaca
     7801 gacgatgtac gatgcgtgga accagcccag cggccccgcc gtgtccgccg gcctgcgg
//
LOCUS       DAOOTK010000129         7853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5784_length_7853_cov_4.922160, whole genome shotgun sequence.
ACCESSION   DAOOTK010000129 DAOOTK010000000
VERSION     DAOOTK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5784_length_7853_cov_4.922160"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK634_12040"
     CDS             complement(<1..225)
                     /locus_tag="PK634_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOW98509.1"
                     /translation="MTIPARFLLAFAIAWLAGSHLTPAAVLISEFMADNQTVLQDEDG
                     DYSDWIEIHNSGPADVDLGGWHLTDDTNQPA"
     gene            complement(314..946)
                     /locus_tag="PK634_12045"
     CDS             complement(314..946)
                     /locus_tag="PK634_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOW98510.1"
                     /translation="MKKLMGIALMLLLSVTARATLVITEAMSSSNHTNTFANGDWWEL
                     YNDDTVAVDLTNYSWDDNSATPGSANFNGLSIGAGEFLIISQETIGEEDSWRTIWGIP
                     SSVAVVNLGNTEFQNFSASGDAIYLYSPSSVQIASITFGTATAGYSFEWDKTGTSLGL
                     SVVGENGAFQSTSMGGGPDVGSPGAIPEPGTLALVAGGLAALLMRRHLQR"
     gene            complement(1092..2414)
                     /gene="miaB"
                     /locus_tag="PK634_12050"
     CDS             complement(1092..2414)
                     /gene="miaB"
                     /locus_tag="PK634_12050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOW98511.1"
                     /translation="MMVFVERTYHIWTIGCQMNEADSRKVAAQLEALGYAPARHAEEA
                     SLVVLNTCVVRQQAEDRAVRRLHPLKALKRKKPGTVIALMGCMVSPRELPELKQRFPW
                     VDVFAPPSDTAPLLDHVEAAHGKTRRAERDAIQDGGPVLPVSQQARAVTAYVPVVLGC
                     SHGCTFCVIPGRRGRERSRPPEEILREARRLADQGVREITLLGQIVDRYGLDLDGKPD
                     LAGLLEQISAIPEVLRIRFLTSHPNWLTDRLLDAVARLPKVCPYIEVPFQSGNDEILA
                     RMKRGYTADDYRRLVGRIREKIPDAAVLTDIIVGFPGETEAQFMDSYRLLDELRLDVA
                     HVAQYSVRPGTVAARTMDDDVPPEEKERRWQMLENRQTEIQAEKNATLLGRTVEVLAE
                     ERAKNGRWRGRTPQNKLVFFEGDGDWLGREASVTLDWTGPFTLIGHCA"
     gene            complement(2492..2914)
                     /locus_tag="PK634_12055"
     CDS             complement(2492..2914)
                     /locus_tag="PK634_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOW98512.1"
                     /translation="MVSNRCQYALRAMVELARQEGKGLATIAEIARARHIPVRFLESI
                     LLTLKQNGFTRSVRGKEGGYLLARSADKISAGDIIRLFEGPLFAPRKTAEAARDVLAT
                     LWGRAETAMSEVFDSVSFRELARLDEQSERSAALHFDI"
     gene            complement(3018..5993)
                     /locus_tag="PK634_12060"
     CDS             complement(3018..5993)
                     /locus_tag="PK634_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW98513.1"
                     /translation="MKTAKTCLKILAFVWFVALLAAPQAMAEKPGTDQPPPSIIVQLS
                     PALAALAAQDLVKPALSPSEFNALVKSLLSRPSWSGQQAFLNALTAIRATGLRAPFRN
                     SKADRTSSPERAALYDTFVIRLANPAASLNAEKLEATAGVLSVEPDAWCRTEWLPNDP
                     FFASAGTWGQPYDDLWALKRDKLDAELAWNLGFGAGILVAVVDSGVDRNHPELNGQVW
                     QNAGEIAANNVDDDGNGFVDDVRGWNFQAGHNDTMDVNGHGTFCAGIIAARGNNARGI
                     IGVAPQAKVMPLTITDAEGDGLISDAAAAIVYAVDKGARVINLSVGDYRKLKVITSAL
                     AVAYQKKVVAVCSAGNDSCDKYHTPGSEVDAICVAASDPADLHCEFSNKGYWLDVCAP
                     GGDPDPFDPCNILSLLSAVHSPDLNPLVIGGQYCRMAGTSASAPYVSGAAALILGLHP
                     DYEVDVVRSLLRASAETADGGPWSYRTGYGRINLNNLVRYNGAGAARIRTPLDRHFRI
                     RPGDAVQFKGKAFCSAMTKWRLEWGHGESPAAWNPINGSVVPQPNGALCANFVFPGAA
                     AARVDQRCVQLTVEGPKQFYQDKVWVTIDPKWKWTKLTGGVNDEAFVDMALDAAGNVY
                     TLCQLGRTDGVDYFFSIAVCKFDGDGNLLWARTKGSNRTFGGGIAVDAAGNVYATGMT
                     KEKLDGQPNNNPDGDMVVGDIFVIKYDTNGNWKWTRIRGSADADAGNKIACDNAGNVY
                     VGGDAKAAFDGQPFQGNADMLVMKYNPAGVWQWTRFWGTPDFDRAHSIVADGAGNTWV
                     TGPTGALLDQSVFVRKYSPAGVPLDSVVHAAPDAGYAIATDLVLDAARNVYLTGYMRG
                     LDWDGEDNPDLLKKKGFAQKYNAALDWQWTRLFGSEENNEGASICLLNGRLMIGAKEV
                     YPVGGFSGHPSTYLISYTTAGALAWRDNIFANSEWKSEPKAVAAKENVLYTAGRTEGP
                     YDGAVYKGMGDGLLMQYKF"
     gene            complement(6139..6918)
                     /locus_tag="PK634_12065"
     CDS             complement(6139..6918)
                     /locus_tag="PK634_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW98514.1"
                     /translation="MPSPLQLEARAVAAGYGDKAILHDISLRFAPGELVGLIGPNGAG
                     KTTLLRVLSGSLRPAQGAVMLGEADLAAMNARRLAQRMAFVPQSLNLPVAFTVRDFVA
                     LGRRPYGAAWSRLAAGDREAIGTALRQADLEGMEEQLVDELSAGERQRTLVALALAQE
                     PQVLLLDEPTAHLDIQHAWRLLELVRALNRERGTTVIFSSHDLNLAAAFCRRLVLLDQ
                     GRLAADGAPEDVLTAGVLSPTYRHALEVIALPGSRRVVVPA"
     gene            complement(7008..>7853)
                     /locus_tag="PK634_12070"
     CDS             complement(7008..>7853)
                     /locus_tag="PK634_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOW98515.1"
                     /translation="HVQAMKTCGHGAVTGAALSVSGVILQALLRNPLAEPYVLGLSAG
                     SGLATALGILLGGLAFSSLMLPAAGFVGGLVSLAIVWRLASAGRITGPHTLVLAGVVW
                     GSLCGSLLMFIVSQASVEGLHAVLWWLLGDLQVFDTRLVLTAAAIVLPALAAAALLAR
                     DLDVLLLGEEMAGHVGLNAERAKIIFLALAALLTAASVCISGLIGFVGLTAPHAARAL
                     VGPNHRRLVPAAALLGAAFLVLADGLGRTLFYPVEIPIGVITSLVGAPFFLLLLRRKQ
                     KEMWR"
BASE COUNT         1303 a         2593 c         2620 g         1337 t
ORIGIN      
        1 ggccggctgg ttcgtgtcgt ccgtgaggtg ccagccgccg aggtccacgt ccgccggacc
       61 ggagttgtga atctcgatcc agtcggagta atccccgtcc tcgtcctgca gcaccgtctg
      121 gttgtccgcc atgaactcgc tgatgagcac ggcggcgggc gtgagatggc tcccggccag
      181 ccatgcgatg gcgaacgcaa ggaggaatcg ggcgggaatg gtcatggctc caacatacgc
      241 cgactgcgcc gggccggtaa agccctttac ctgtagaaaa acaggttgtc cggcctagtt
      301 gaaggaaaag ccgttaccgc tgaaggtggc gacgcatcaa cagggccgca aggccgcccg
      361 ccaccagcgc cagcgtgccc ggctccggaa tcgcgcccgg cgacccgaca tctggtccgc
      421 cgcccatgga cgtggactgg aacgcgccgt tctcgccgac gacgctcaag cccagcgatg
      481 tgccggtctt gtcccattcg aaggaataac ccgccgttgc cgtgccaaag gtgatggagg
      541 cgatctggac ggaagaaggg ctgtaaagat agatggcgtc gccggaggcg ctgaaattct
      601 gaaattcggt gttgcccagg ttgacgaccg ccacgctgga cggaatgccc cagatcgttc
      661 gccagctatc ctcttctcca atggtttcct ggctgataat caggaactcc ccggcgccga
      721 tcgacagccc attaaagttg gccgagccgg gcgttgccga gttgtcgtcc cacgaataat
      781 ttgtcaggtc cacagccacc gtgtcgtcat tgtacaattc ccaccagtcc ccattcgcga
      841 acgtgttggt atggttgctc gaagacatcg cctccgtgat gaccagcgtg gcgcgggccg
      901 taacggacag aagcagcatc agcgcgattc ccatgagttt tttcatgcgt tccttttctc
      961 gcggtcgagt ggggttcagg atcaatcgct ttactttgaa taaatatata ccccgtggac
     1021 attaagcggc aattaagttc cgaaaaaaaa cgaagggcag gttctttcaa cgcgagccgc
     1081 cgcgcgcggt ttcaggcaca atggccgatc agtgtgaaag gccctgtcca gtcgagagtt
     1141 acagaagcct cccggcccaa ccaatcgccg tcgccttcga aaaaaaccag cttgttctgc
     1201 ggcgtccggc cgcgccagcg tccgtttttc gcgcgctctt cggccagcac ttcgacggtt
     1261 cggcccagca gcgtggcgtt cttctccgct tggatttcgg tctgccgatt ctccagcatc
     1321 tgccagcgcc gctccttttc ctccggcggc acgtcgtcgt ccatcgtccg ggccgccacg
     1381 gtgcccggcc gcacggagta ctgcgcgacg tgcgccacgt ccagccgcag ctcgtccagc
     1441 agccggtacg aatccatgaa ctgcgcctcg gtctcgcccg ggaacccgac gatgatgtcc
     1501 gtcagtacgg cggcatcggg gatcttttcg cgaatccggc cgaccagccg ccggtaatcg
     1561 tccgccgtgt acccgcgctt catccgggcg aggatctcgt cgttgccgga ctggaacggc
     1621 acctcaatgt agggacagac cttcggcagg cgcgcgacag cgtcgagaag ccggtcggtc
     1681 agccagttgg gatggctggt cagaaaacgg atgcgcagta cttccgggat cgcgctgatc
     1741 tgctccaaca ggccggcgag gtccggcttc ccgtccaggt cgagcccgta gcggtccacg
     1801 atctggccga gcagtgtgat ctcccgcacg ccctggtccg ccaggcgtcg cgcctcgcgc
     1861 aggatttctt cgggcgggcg gctgcgttcg cgcccgcgcc ggccggggat gacgcagaat
     1921 gtgcagccgt gcgagcagcc gagcacgacg gggacgtagg ccgtgacggc ccgcgcctgc
     1981 tgcgaaaccg gcagcacggg gccgccgtcc tggatcgcgt cgcgctccgc gcggcgggtc
     2041 ttgccatgcg cggcctcgac gtgatcgagc aggggggccg tgtccgacgg cggggcgaag
     2101 acgtccaccc acgggaagcg ctgcttcagc tcgggcagtt cgcggggcga gaccatgcag
     2161 cccatgagcg cgatgaccgt gccgggtttt ttccgcttga gcgccttcag cggatgcagc
     2221 cgccggacgg cgcggtcctc ggcctgctgc cggacgacgc aggtgttgag cacgacgagc
     2281 gacgcctcct ccgcgtgacg ggcgggcgcg tagcccagcg cctcgagttg cgcggcgacc
     2341 ttgcgggagt cggcctcgtt catctggcag ccgatggtcc agatatggta cgtgcgctcc
     2401 acgaacacca tcatacagga atcaaccgcg gctgtcgcgg ctggagggag agatcgcctt
     2461 tacgcccttt gcgccttctg ttccatcctc ttcagatatc gaaatgcaga gcggcggacc
     2521 gctcgctctg ctcgtccaat cgggcgagct cgcggaacga aacggaatcg aacacctccg
     2581 acatcgccgt ttccgcgcgg ccccacaggg tggccaggac gtcccgggcg gcctcggcgg
     2641 tcttgcgcgg ggcgaacagg ggcccttcaa agagtcgtat gatatctccg gccgaaatct
     2701 tgtccgccga ccgggccagc aggtatccgc cctccttgcc ccgcacggac cgggtgaagc
     2761 cgttctgctt cagggtcagc aggatggatt ccaggaagcg cacgggaatg tgccgagcgc
     2821 gcgcgatttc cgcgatcgtg gccaatccct tgccttcctg ccgggccagt tccaccatcg
     2881 cccggagcgc gtattggcag cggttcgata ccatgtccgc aatatacact aaggcggtag
     2941 attatccaga caggccaagt atataaaaaa agggggaggc cggcttggcg cacggccccc
     3001 ccggggtggg cgaacgtcta gaacttgtac tgcatcagca acccatcgcc catgcctttg
     3061 tacacggctc cgtcataggg accttccgtc cgtccggcgg tgtacaggac gttctccttg
     3121 gccgccacgg ctttgggttc ggacttccat tcgctgttcg cgaatatatt gtcccgccaa
     3181 gcgagcgcgc ccgccgtcgt gtagctgatc aggtacgtcg acggatggcc ggagaagccg
     3241 ccgaccggat aaacttcctt ggcgccgatc atcagccttc cgttcagcag gcagatggac
     3301 gcgccttcgt tgttctcctc ggatccgaac aggcgtgtcc actgccagtc aagggccgcg
     3361 ttgtattttt gggcaaagcc ttttttcttg agcaggtcgg gattgtcctc tccgtcccag
     3421 tcaagcccgc gcatatagcc ggtcaggtag acgttccggg cggcgtccag gaccaggtcc
     3481 gtcgcgatgg catagccggc gtccggcgcc gcgtgcacga cggagtccag cggaacgccg
     3541 gccggactgt acttgcggac gaagacggac tggtcgagca gcgcgccggt cgggccggtc
     3601 acccacgtgt tgcctgcgcc gtccgccacg atcgagtggg cccggtcgaa gtccggcgtg
     3661 ccccagaagc gcgtccattg ccagacgccc gccggattgt acttcatcac cagcatgtcg
     3721 gcattgccct ggaacggctg gccgtcaaag gcggccttgg catctccgcc gacgtagacg
     3781 ttcccggcgt tgtcgcaggc gatcttgttc cccgcgtccg cgtcggcgct gccccggatg
     3841 cgcgtccact tccagttccc gttcgtgtcg tacttgatca cgaagatgtc gccgacgacc
     3901 atgtccccgt ccggattatt gttcggctgg ccgtcgagct tctccttggt catgcccgtc
     3961 gcatacacat tgcccgcggc gtccaccgcg atccccccgc cgaacgtccg attgctgcct
     4021 ttcgtcctgg cccacagcag gtttccgtcg ccgtcgaact tgcagaccgc gatcgagaag
     4081 aagtaatcca ctccgtccgt cctgccgagc tggcagaggg tatacacgtt gccggcggcg
     4141 tcgagggcca tgtccacaaa cgcctcgtcg tttacgcccc cggtgagctt ggtccatttc
     4201 cacttcggat cgatcgtcac ccacaccttg tcctggtaga actgcttggg cccctcgacc
     4261 gtgagttgca cgcagcgctg gtccacgcgg gcggctgccg cgccggggaa aacgaagttg
     4321 gcgcagagcg cgccgttggg ttgcggcacg acgctgccgt tgatcgggtt ccaggccgcc
     4381 gggctttcgc cgtgccccca ttccaggcgc cacttcgtca tcgccgagca gaacgccttg
     4441 cctttgaact gaacggcgtc gcccggccgg atccggaaat gccggtccag cggcgtgcgg
     4501 atgcgggccg cgccggcccc gttgtacctg accaggttgt tgagattgat gcgcccgtag
     4561 cccgtccggt aggaccacgg cccgccgtcg gcggtctcgg ccgaggcgcg cagcagggag
     4621 cgcacgacgt ccacctcgta gtccgggtgc agaccgagga tcagcgccgc ggcgccggac
     4681 acgtagggcg cggaggcgga cgtgcccgcc atgcggcagt actgtccgcc gatcacgagg
     4741 gggttcaggt cgggcgagtg cacggccgag agaagggaga ggatgttgca gggatcgaag
     4801 ggatccgggt cgccgcccgg ggcgcagacg tccagccagt agcccttgtt gctgaattcg
     4861 cagtgcaggt cggccgggtc gctggcggcc acgcagatcg cgtccacctc gctgcccggg
     4921 gtgtggtact tgtcgcagga gtcgttgccg gcggagcaga cggcgaccac cttcttctgg
     4981 taggcgacgg ccagggccga ggtgatgacc ttcagtttcc tgtagtcgcc gacgctcaag
     5041 ttgatcacgc gggcgccctt gtccacggca tagacgatcg ccgcggccgc gtcgctgatc
     5101 aggccatccc cctccgcgtc ggtgatcgtc agcggcatga ccttggcctg gggcgccacg
     5161 ccgatgatac ccctcgcgtt gttcccgcgg gcggcgatga tccccgcgca gaaggtgccg
     5221 tggccgttca cgtccatcgt gtcgttgtgg ccggcctgga agttccagcc gcggacgtcg
     5281 tccacgaacc cgttgccgtc gtcgtccacg ttgttggccg cgatttcccc ggcattctgc
     5341 cagacctgtc cgttcagctc gggatggttc cggtccacgc cggagtccac gaccgcgacg
     5401 aggatgcccg cgccgaagcc caggttccac gccagttcgg cgtccagctt gtcgcgcttg
     5461 agcgcccaca ggtcgtcgta gggctggccc caggtgccgg cgctggcgaa gaagggatcg
     5521 ttggggagcc attccgtgcg gcaccacgcg tcgggctcga ccgacagcac cccggccgtc
     5581 gcctcgagtt tctccgcgtt cagggacgcc gccgggttgg ccagccggat gacgaacgtg
     5641 tcgtacagcg ccgcccgttc cgggctcgaa gtgcggtcgg ccttggaatt acggaaaggc
     5701 gcgcgcagcc ccgtcgcgcg gatggccgtc agggcgttga ggaaggcctg ttgcccggac
     5761 cacgaggggc gcgacaggag cgatttcacc agcgcgttga actcggaggg ggacagggcg
     5821 ggcttgacca ggtcctgcgc ggccagggcc gccagggcgg gggacaattg aacgatgata
     5881 ctgggcggcg gctggtcggt tcccggcttt tcggccatgg cctgcggcgc ggcgagcaag
     5941 gcgacgaacc aaacgaacgc aaggatcttc aggcatgttt tcgcggtctt catgggccct
     6001 ccggttcctg gtttcctcgg cgaagcgagg cttaaacatt tacggcatac ggctgcatgc
     6061 tcaaggaccc cagtcgcgcg tggagatgct ttcttcccgg ctttcggaaa aaatgaggcg
     6121 acccggccgg cgacaggatc aggccggcac gacgacgcgc cgggaaccgg gcagcgcaat
     6181 cacctcgagg gcatgacgat aggtcgggga aaggacgccc gccgtaagga cgtcttccgg
     6241 cgccccgtcg gccgcgaggc gcccctggtc aagcaggacc agccggcggc agaaggcggc
     6301 ggcgaggttg aggtcgtgcg aggagaagat cacggtcgtg ccccgttcgc ggttcagcgc
     6361 gcggaccagt tccagcaggc gccaggcgtg ctggatgtcc aggtgcgccg tcggctcgtc
     6421 gagcagcagg acctgcggtt cctgcgcgag ggcgagggcg acgagcgtcc gctgccgctc
     6481 gccagcgctc aattcgtcca cgagttgctc ctccatgcct tccaggtccg cctgccggag
     6541 ggcggttccg atcgcctcgc gatcgccggc agccaggcgg ctccatgccg ccccgtatgg
     6601 ccggcggccg agcgcgacga agtcccggac ggtgaaagcc acggggaggt tcaggctctg
     6661 cggcacgaag gccatccgtt gcgcgaggcg gcgcgcgttc atcgcggcga ggtcggcctc
     6721 gcccagcatc accgcgccct gcgcggggcg cagggagccg ctcaagacgc gtagtagcgt
     6781 ggtctttccc gcgccgtttg gcccgatcag gccgacgagt tccccgggcg cgaagcgcag
     6841 ggaaatatcg tgcaggatcg ccttgtcgcc gtatccggcg gccacggcgc gggcttcgag
     6901 ttggagagga gagggcacgg atcagacgtg aatggtgaat ggtgaatggt gaatagtgga
     6961 ggcccaacct cctcctgaca cctgaaccct gaaccctgac accttcatta tctccacatc
     7021 tccttctgct tccggcgcag cagcaacagg aagaacggcg cgcccacgag ggacgtgatc
     7081 accccgatgg ggatttcgac gggatagaac aacgttcgcc caagcccgtc ggccaggacg
     7141 aggaaggcgg cgcccagcag cgcggcggcg gggacgagtc gccggtggtt cgggccgacg
     7201 agggcgcgcg cggcgtgggg cgccgtgagc ccgacgaacc cgatcaggcc gctgatgcac
     7261 acggaggcgg cggtcagcag ggcggccagg gcgaggaata tgatcttggc gcgctccgcg
     7321 ttcagcccga catggcccgc catctcctcg ccgagcagca ggacgtccag atcccgggcc
     7381 agcagggcgg cggccgcgag ggcgggcagg acaatcgcgg cggccgtcag gaccagccgc
     7441 gtatcgaaga cctgcaaatc gccaagcagc caccagagga cggcgtgcag cccctcgacg
     7501 ctggcctgcg agacgatgaa catcaggagg cttccgcaca gcgaacccca caccacgccg
     7561 gcgaggacga gcgtgtgcgg ccccgtgatc cgccccgccg acgccaggcg ccagacgatg
     7621 gccaggctga ccaggccgcc gacgaagccg gccgccggca gcatcaggct gctgaacgcg
     7681 agcccgccga gcaggatccc cagcgcggtg gcgaggccgg agccggcgga cagcccgagc
     7741 acgtacggct ccgccagcgg gttgcgcagg agcgcctgca ggatcacgcc cgacaccgag
     7801 agcgccgcgc ccgtgacggc accgtggccg catgttttca tcgcctgaac gtg
//
LOCUS       DAOOTK010000130         7845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5792_length_7845_cov_4.647112, whole genome shotgun sequence.
ACCESSION   DAOOTK010000130 DAOOTK010000000
VERSION     DAOOTK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5792_length_7845_cov_4.647112"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PK634_12075"
     CDS             <1..1198
                     /locus_tag="PK634_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98516.1"
                     /translation="YNADLPLTITNSGSYVLAEDAGSVSSVSIQITVDADDVVLDLNH
                     SMLTGSGFAADYCVVQSAGRNNLTIKNGRISGSLNIPGAFNRCEDLFIRDGRRNAINT
                     GPGSLIARCTLAGNFITALGIGLICADAGSIIRDCMMSNNMGGDELAGIRAGDGSLIE
                     RCVISEQSGGRVYGVIAGTGAILDGVVIRDLLGSAATNNTAVVAGNGALLTGCTLAGN
                     VDLGTGSVVADCLFTGMDTVHSYQASVGHGSVVVDNVFSGQVAGTDAVNINSGSLAVN
                     NRLSQYRLQADNGSLIAQNWADVPVFLKYDCYVYDNFLGRGISETGGWNRIEGNYLDR
                     TSLHGTNVCINSFGQRNLIVKNFFAEDEDHVISGGANDHIAASIIPPGNTFTTNRPWG
                     NVVRLE"
     gene            1202..1867
                     /locus_tag="PK634_12080"
     CDS             1202..1867
                     /locus_tag="PK634_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98517.1"
                     /translation="MSLRFQKWMLMTALAGATAVATRAGPGDVQINTLPDIIVEPGRY
                     MVTRNLFLPAGIVGTGIQVRATGVTIDLNGFTLDGSNVACGIVQDADTDDLTVLNGYI
                     RSCAQAVQAPGAGNRFKDLGITACSKGITAGNSAVVEGCIFGQNSSADLSLITVGEGS
                     RVSECLMGINSGSTAFEGIECGEGSEVRRILFTPTHFRGRRRIWFAARTWWNRCWPMI
                     IVS"
     gene            1888..2493
                     /locus_tag="PK634_12085"
     CDS             1888..2493
                     /locus_tag="PK634_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98518.1"
                     /translation="MTIRDCVITETAAQDDSGLFSDGETAERCVVYGNRSGQAGGSSP
                     VGFWDVRKVLDSVAANRTSSDMLSGDGFNNCDTLGCVAYAHAGDGFQGSVFQYSVAFT
                     NVKVGFYLGANVSAQNCLAVANESGFVDQNLVSSLRVVDNHAARNPAVGFWLQRPGVI
                     AVDNSAQGNFTIGGSAKGGAGIVLTGGTTINTNNAWANFSW"
     gene            2501..3823
                     /locus_tag="PK634_12090"
     CDS             2501..3823
                     /locus_tag="PK634_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98519.1"
                     /translation="MQRRRTETERGGRLGFGLALLMLGAAVEGARAADGRFEISQSHV
                     PYTISRPGSYVVTEDLDSPSGDAPVIFIATNYVTLDLNGHRLQQAYDHAIIQSSGGCS
                     YITVRHGRLLHVNNLARALELNSLFNRVEDVEICGFNETGLFSADYTLLKRCRVVGGE
                     GFNGINSGQGSMVLACEAAGNTAYLHGTGISGDHGFLVVDSIVSGWRGTGIGSFIGFW
                     GDTGYLCVNNVVSDNQLDALTVEAFRLGSGGLVAGCTAQGNQDALTNAAGILARDGVV
                     VADCSIEGRNYPGITTSNGTIVARNALKGPNCDLKTGQGSVVRGNTLHEGGINVQEGC
                     LVAGNSVWRDVYMNPLLLLAGAGYALENNLWGWDTPGIQMVGKNSRADRNNISTVTDI
                     RVDLGSSNLIVRNRFNAMTIIASNDFVGATLVGSEGMSANLPWANIDW"
     gene            3825..5189
                     /locus_tag="PK634_12095"
     CDS             3825..5189
                     /locus_tag="PK634_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98520.1"
                     /translation="MKAFRTIRLALLMIGLLSGGARAAALSTQEGVREISQATVITSP
                     GAYLVTRDLLVLGGSAITIQADDVTLDLNGHRIQSVPVFGTYDAAIRQQDWFANLSVF
                     NGTVIGGAGAGIWATGRNSRVENVTVRGSQKGGILLGDMAVVENCIVVSNDLGLTGTN
                     LICGGAGSRVAGSILIGNTGARLVGIRGGQGCIVDGCILRGNLASYYFNGVELARGGV
                     VRSVSISDNYCASSFSGVVADGTIERCAVVRNIKDMDGGSLAGIDAGVNGVVRRCIVK
                     ENTAYSGEAYAYFHRGHGGLFDCVAFSNVAVGASAYGFCLSTNNTVASRCAATANGSR
                     GFYGPIGCIIKQSVAKWNGWYGIDVGAWSSVERCLVDNTGDATDTRTGIAANNGNVRV
                     ENNHVLDSGRGIGCFGGVNFLSGNSAVNTTNYVNTTGQSCGAITNQPGANFISYNPWQ
                     NFGL"
     gene            5255..6427
                     /locus_tag="PK634_12100"
     CDS             5255..6427
                     /locus_tag="PK634_12100"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOW98521.1"
                     /translation="MALQAALCMVLFLLGPAAWGAGLGSNEVRLVVDSAARVGSMDCS
                     DETIVSIGPGAMGTLPSLQLPGNGRAAVDAMAAAGKYIIFSCDVDVRINGIRYADEDL
                     ILFDPTTTNYSMFFQGASYGLPEACDMDAVAVSDNFDVEGSDGFVFLSTDIDVPEWNI
                     TDDDVLIWWRIGAPAKQLDGRTQLGIPERANLDALHYISPYLYYSLDTYETIGPATAR
                     DRDAFAWDGSNFSAVKFEQIEPQANLAALDYPMDRDHDWLTDFEEASGIDEGATTVPG
                     TTVPLNPNGHKTKPDVADSDGDGVPDGEEAAAGTNPNDLNDYLQLTTMQEVSSNQLVS
                     WASVAGRYYDLEAITNWFLGHPSVVADDVQASGATTTRTNVGAQSILFYRVRLEPR"
     gene            6442..6684
                     /locus_tag="PK634_12105"
                     /pseudo
     CDS             6442..6684
                     /locus_tag="PK634_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6907..>7845)
                     /locus_tag="PK634_12110"
     CDS             complement(6907..>7845)
                     /locus_tag="PK634_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW98522.1"
                     /translation="AAARLFDIPPDKARNRLLQELVRNPALHQLVADAVARGEPAEDD
                     ITVFNGRERHLRVRASRLRDAQGEGIGALVVFHDVTRLRRLESVRRDFVANVSHELKT
                     PITSIKGFIETLQDGALKSEEEARRFLGIMARQADRLNAIIEDLLQLSRIEEEADQQV
                     IRLEEQEVSPVLRSAVECCQYKADARQMAVELDGAPGLRARFNTPLLEQAVINLLDNA
                     IKYSPSGSPVRVEAGREGADVVIRVRDRGPGIPPEHLPRLFERFYRVDKARSRQMGGT
                     GLGLAIVKHIAQAHGGNVSVESIPGQGSVFSLRIPA"
BASE COUNT         1447 a         2289 c         2638 g         1471 t
ORIGIN      
        1 ttataacgcg gatcttcccc tcacgatcac caactcgggc agctacgtcc tggcggaaga
       61 tgccggatct gtttcatccg taagcattca gatcaccgtg gatgccgatg acgtcgttct
      121 ggatctgaac cacagcatgc tcaccggcag cggtttcgcg gcggactatt gcgtggtgca
      181 gtccgccggt cgaaacaact tgaccatcaa gaacgggcgg atttcagggt cgctcaatat
      241 ccccggcgcc ttcaatcgct gcgaagatct ttttatcaga gatggcaggc ggaacgcgat
      301 caacacgggg cccggcagcc tgatcgcgag atgtacgctg gcaggcaact ttattacggc
      361 gttgggaatt gggctcatct gcgccgatgc aggttcgatc atccgggatt gcatgatgtc
      421 caacaacatg ggcggtgatg aactcgccgg gattcgcgcg ggggatggca gcctgatcga
      481 acgctgcgtg atcagtgaac agtcgggcgg gcgagtctat ggggtgatcg cggggacggg
      541 agcgatcctc gacggcgtgg taattcgcga tctactcggt agcgcggcga ccaataacac
      601 tgcggtggtt gccggcaatg gggcattgct gaccggctgc acactggccg ggaatgtgga
      661 cctcggcacg ggatccgtcg tcgcggattg cctgttcacc ggaatggaca cggtccattc
      721 ctatcaggcc agtgtggggc acggctcggt cgtggtggat aatgtcttct ccggccaggt
      781 ggcggggacg gacgcggtca acatcaacag cggctccctg gcggtcaaca accggttgag
      841 ccagtaccgc ctgcaggccg acaacgggag cctcatcgcc cagaactggg ccgatgtccc
      901 cgttttcctt aagtacgact gctatgtgta tgacaatttc ctcgggcgcg ggatttcgga
      961 aacaggcggc tggaatcgaa tcgagggcaa ctatctggac cgcacctcgc tgcacggaac
     1021 caatgtctgc attaattctt tcggccagcg caacctgatc gtgaagaatt tctttgcgga
     1081 ggatgaggac cacgtcatct cgggcggggc gaacgaccat atcgcggcca gcatcatacc
     1141 gccggggaac accttcacga ccaaccgccc ttgggggaat gtggtgcgac tcgaataaca
     1201 tatgagcctg cgtttccaga agtggatgct gatgaccgcc ctggcggggg ccacggccgt
     1261 ggcgacccgg gccgggccgg gcgatgtgca gattaacacc ttgcccgaca tcattgtcga
     1321 gcccggccgg tacatggtga cccggaatct tttcctgccg gcggggatcg tgggcaccgg
     1381 catccaggtg cgagctaccg gtgttacgat tgacctgaac ggctttaccc tcgatggcag
     1441 caatgtcgcc tgcgggattg ttcaggacgc agatactgat gacttgaccg tgctcaacgg
     1501 gtacatcagg agctgcgcgc aggccgttca ggcgccgggc gccggcaatc gtttcaagga
     1561 tcttgggatc acggcttgct ccaagggaat caccgccgga aactcggccg tggtcgaggg
     1621 ctgtatcttc gggcagaatt cgtcggcgga cctgtccctg atcaccgtgg gcgagggcag
     1681 ccgggtcagc gaatgcctga tgggcatcaa ttccggttcc accgccttcg aagggatcga
     1741 gtgcggcgag gggtcggagg tccggcgcat cctgtttacg ccaactcact tcaggggacg
     1801 ccgtcgtatc tggttcgcgg cgcggacctg gtggaacagg tgctggccca tgataatagt
     1861 ttcctgaaac agggcatcgt cagtgccatg acgattcggg attgcgtcat taccgaaacg
     1921 gcggcgcagg atgatagcgg gttgttcagt gacggcgaga cggcggagcg ttgcgtggtg
     1981 tatggcaacc ggtccggcca ggcgggcgga tcctctcccg tcggcttctg ggatgtccgg
     2041 aaggtcctgg acagcgtggc ggcgaatcgc accagcagcg acatgctctc gggggatgga
     2101 ttcaacaact gcgacaccct gggctgcgtg gcgtacgcgc acgccgggga cggcttccaa
     2161 ggcagcgtgt tccagtactc cgtggcgttc acgaatgtca aagtcgggtt ctatctcggc
     2221 gccaacgtga gcgcgcagaa ttgcctcgcc gtggccaatg aatcgggctt cgtggaccag
     2281 aacttggtca gttccctgcg agtggtggat aatcatgcgg cgcgcaaccc ggccgtcgga
     2341 ttctggctgc agcggccggg cgtgattgcg gtggacaaca gcgcgcaggg caacttcacg
     2401 atcggcgggt ccgccaaggg cggcgccggc atcgtgctga ccggcgggac gaccatcaac
     2461 acgaacaacg cgtgggcgaa tttctcgtgg tagagtaagg atgcaaagac gacgaacaga
     2521 aacggagcga ggagggcggc tggggttcgg cctcgcgttg ctcatgttgg gcgcggcggt
     2581 cgagggagcc cgcgccgccg acggccgttt cgaaatctcg caatcccacg ttccgtacac
     2641 catctcgcgt cccggcagtt atgtcgtgac cgaggatttg gacagcccga gcggcgacgc
     2701 gccggtgatt ttcatcgcga ccaattatgt gacgctggac ctgaacggcc accggttgca
     2761 gcaggcatac gaccatgcca tcatccaatc ctccggcggt tgttcctata tcacggtccg
     2821 acacggccgg ttgctccatg tcaacaacct ggcccgggcg ctggaattga acagcctttt
     2881 caaccgcgtg gaggacgtgg agatttgcgg attcaatgaa acggggctgt tcagcgcgga
     2941 ttacacgctt ctgaagaggt gccgcgtagt cggcggcgag ggctttaacg gaatcaacag
     3001 cgggcagggg agcatggtcc tggcctgtga agccgccgga aacaccgcgt acctgcacgg
     3061 gaccggaatc agcggcgatc atggctttct cgtggtggac agcatcgtga gcggctggcg
     3121 cgggacgggg ataggatcgt tcatcggctt ctggggcgac acggggtact tgtgcgtcaa
     3181 caatgtggtg agcgacaacc aactggatgc gctgaccgtc gaggccttcc ggctgggcag
     3241 cggcgggttg gtcgcgggtt gtacggcgca ggggaatcag gatgcgctaa ccaatgccgc
     3301 cgggattctc gctcgagacg gcgtggtggt ggccgactgc tccatcgaag ggcgcaacta
     3361 ccccgggatc acgacatcca acggaaccat cgtggcgcgc aacgcgttga aggggccgaa
     3421 ctgcgacctg aagacgggcc agggaagcgt tgtccggggg aataccctgc acgagggcgg
     3481 catcaacgta caggaggggt gtctcgtggc cgggaattcc gtctggcgcg atgtctatat
     3541 gaacccgctc ctccttctcg ccggcgccgg ctacgcgctg gagaataacc tgtggggctg
     3601 ggacacgcca gggattcaga tggtgggcaa gaacagccgg gcggatcgga acaatatcag
     3661 cacggtcacg gacatccgcg tggacctcgg gtcaagcaat ctgatcgtgc gcaaccgttt
     3721 caacgccatg acgatcatcg cgtccaatga ctttgtcggc gcgacgctgg tggggagcga
     3781 gggaatgtcg gcgaatttac cgtgggcgaa tatagactgg taacgtgaaa gctttcagaa
     3841 caatacggct ggccctgctc atgatcgggc tgctctccgg cggcgcccgg gcggcggccc
     3901 tttccacgca ggagggcgtg cgcgaaatca gccaagccac cgtcatcacc agtccgggcg
     3961 cgtacctggt cacccgggat ttgctggtgc tcggcggaag cgccatcacc attcaagcgg
     4021 atgatgtgac cctggatctg aacggacacc ggatccagtc ggtgccggtt tttggcacct
     4081 acgacgcggc gatccgccag caggactggt ttgcgaatct ttcggtgttc aacggcaccg
     4141 tgatcggcgg ggccggggcg ggtatctggg ccaccggccg gaacagccgc gtggaaaacg
     4201 tgaccgtccg gggcagtcag aagggcggga ttctcctggg cgacatggcc gtggtggaga
     4261 actgcatcgt ggtatccaat gacttaggtt tgaccggcac caatctgatt tgcggagggg
     4321 cgggcagccg cgtggccggg tccatcctga tagggaatac cggcgcccgc ctcgtgggca
     4381 tccggggcgg gcagggttgt atcgtggacg gctgcatcct ccgcggcaat ttggcctcgt
     4441 actatttcaa cggtgtggag ctggcacggg gcggcgtggt ccgcagcgtc agcatcagcg
     4501 acaattactg cgcctccagt tttagcggcg tggtcgccga tggaacgatc gaacgctgcg
     4561 cggtggttcg gaacataaag gatatggacg gtggcagcct ggcgggtata gacgccgggg
     4621 tcaacggggt ggtccgccgc tgtatcgtca aggaaaacac cgcgtatagc ggcgaggcct
     4681 atgcctactt ccaccggggt cacggcggtc tcttcgactg cgtggccttc agtaatgtcg
     4741 cggtcggcgc ctcggcgtat ggattctgcc tgtccaccaa caataccgtg gcgagccgct
     4801 gcgccgcgac cgccaacggt tcgcgaggat tctacggacc gataggttgc atcatcaaac
     4861 aaagcgtggc gaagtggaac gggtggtacg gaatagacgt gggggcctgg tcttccgtgg
     4921 agcgttgcct ggtggacaat acgggagacg ccacggacac gcgaaccggc attgcggcca
     4981 acaacggcaa tgtccgggtg gaaaacaacc acgtgctgga cagcgggagg ggaatcggct
     5041 gctttggagg agtcaacttc ctctccggga acagcgccgt caacactacg aactacgtca
     5101 acacgacggg ccaaagctgc ggcgccatca cgaatcaacc gggcgccaac ttcatatcgt
     5161 acaacccgtg gcagaacttc ggcctatgaa acattttctc cagagtggcg cgggcgtctc
     5221 gcccgtacgt tcacgggccg gagtcccgtg ccacatggca cttcaagccg cactatgtat
     5281 ggtgttgttt ttgttggggc cggccgcctg gggcgccggg ttggggagca atgaagttcg
     5341 gctggtggtg gattccgcgg cccgcgtcgg ctccatggac tgctctgatg aaaccatcgt
     5401 cagcattggt ccgggcgcca tgggaaccct gccgtcactc cagttgcccg gcaacggtcg
     5461 ggcggccgtg gatgcgatgg ccgccgccgg caagtatatc attttttcct gcgacgtgga
     5521 cgtccggatc aacggcatcc gctacgccga cgaggatctc atcctgtttg atccgaccac
     5581 caccaattat tccatgttct tccagggggc ctcctatggc ctgccggagg cgtgcgatat
     5641 ggacgccgtg gccgtgtcgg acaacttcga tgtggagggt tcggacggct ttgttttcct
     5701 gtccacggat atagatgtgc ccgagtggaa tatcaccgat gacgatgtcc tgatctggtg
     5761 gcgcatcgga gccccggcca agcagttgga cggccggact cagttgggca tccccgaaag
     5821 ggccaacctc gacgcccttc attacatcag tccctatctg tattattccc tggacacgta
     5881 tgagacgatt ggcccggcca cggcccggga ccgtgacgca ttcgcctggg acggcagcaa
     5941 tttctcggcc gtcaagtttg agcagatcga gccgcaggcc aacttggcgg cgctggatta
     6001 tccaatggac cgggaccatg attggctgac ggatttcgag gaagcctcgg gcattgacga
     6061 gggggccacg acggtgcccg gcaccaccgt gccgctgaat cccaacgggc ataagaccaa
     6121 gccggacgtg gcggacagtg acggcgacgg cgtgcccgac ggcgaggaag ccgccgccgg
     6181 gaccaatccg aatgacctta acgactattt gcagttgacg accatgcagg aagtgtcgag
     6241 caaccagttg gtgtcctggg catcggtggc gggccggtat tacgatctgg aggcgattac
     6301 caattggttc ctgggccatc cctccgtggt agccgacgat gtccaggcca gtggtgccac
     6361 gaccactcgc accaatgtgg gcgcacaatc catcctcttt taccgcgtgc gcctggaacc
     6421 gcgataaaaa aggagaagtt catgaggcga gccttcgctg tgagtgcctt gttcatcacc
     6481 ctgctggccc tggccggctg cgcggcgggc gacgcgcgtt tcgaggccgc gccagcgggc
     6541 ttctgggccg ggttgtggca tgggctgatc tgctgcatca cgttcattgt cagcctgttc
     6601 aaagacaacg tccacgtcta cgaggtccgc aacgccgggc acctatacga cctcgggttt
     6661 gtgcttggcg cggccatcgc cttcggcggc tgcggcggct cgggctcctg caagtggcgc
     6721 aagtcgcccc gggaaaagga atgggaggat ctcggcgcgc gcgtggaagt caaggtgcgc
     6781 aagggcatcc gcgagtggct cgacgagtcc gggaagaagg atgccgagtg ggaggagatc
     6841 ggccggaagg ttgaggagaa gatcaagcgc gagctgcgga attgggcgga ccaatagggt
     6901 ttttgttcat gccgggatgc gcaggctgaa cacgctccct tgcccgggga tgctttcgac
     6961 cgacacgttc ccgccgtgcg cctgggcgat gtgtttaacg atggccaggc ccagcccagt
     7021 gccgcccatc tgccggctgc gcgccttgtc cacccggtaa aaccgctcga aaagccgcgg
     7081 caggtgctcg ggcgggatcc ccggcccccg gtcgcgaacg cggatgacca cgtcggcgcc
     7141 ctcgcgcccg gcttccacgc ggacgggaga gcccgacgga ctgtacttga tcgcgttgtc
     7201 gagcaggttg atcacggctt gctccagcag cggggtgttg aagcgggccc gcagcccggg
     7261 cgcgccgtcc agttccacgg ccatctgccg ggcgtccgcc ttgtactggc agcattccac
     7321 ggcggagcgc agcaccggag agacttcctg ctcctccagc cggatgacct gctgatcggc
     7381 ctcctcttcg atgcgcgaca gctgcagaag gtcctcgatg atggcgttga gccggtcggc
     7441 ctgccgggcc atgatgccga ggaagcgtcg cgcctcctcc tcgctcttca acgcgccgtc
     7501 ctgcagggtc tcgatgaagc ccttgatcga ggtgatcggc gttttcagtt cgtgggatac
     7561 gttggcgacg aaatcgcggc ggacgctctc cagccggcgg agtcgggtga cgtcgtggaa
     7621 caccaccagc gccccgatgc cctcgccctg ggcgtcccgc aggcggctgg cgcgcacgcg
     7681 caggtgccgc tcgcggccgt tgaagaccgt gatatcgtcc tccgcgggtt cgccccgcgc
     7741 gacggcatcc gcgaccaact ggtggagggc ggggttccgg accagttcct ggagcaggcg
     7801 gttccgcgcc ttgtcgggcg ggatgtcgaa gagccgcgcc gccgc
//
LOCUS       DAOOTK010000131         7829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5810_length_7829_cov_5.283895, whole genome shotgun sequence.
ACCESSION   DAOOTK010000131 DAOOTK010000000
VERSION     DAOOTK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5810_length_7829_cov_5.283895"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="PK634_12115"
     CDS             <1..153
                     /locus_tag="PK634_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOW98523.1"
                     /translation="VRKGLIGKIGENMAVRRFPANLFAGLFGFDPKKNYFEAEEGAEK
                     APTVAF"
     gene            complement(273..851)
                     /locus_tag="PK634_12120"
     CDS             complement(273..851)
                     /locus_tag="PK634_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOW98524.1"
                     /translation="MKYNTLSGLHPTLWRTCRALANERRLDILRLLIAREPLTVSDVA
                     REMDISMATASQYLRSLNARGLLTVMRKKRFVYYTVGADSSLPHAAALLGSVMQSLRV
                     KQNTAGVFMQALTAFTHPRRVQLVKLLAGKPMGVLELLGATGYSLPALSRHLSKLQRR
                     GIVRRRHRKYECMRPSDPLSKTLILLARQDSA"
     gene            962..2710
                     /gene="ilvD"
                     /locus_tag="PK634_12125"
     CDS             962..2710
                     /gene="ilvD"
                     /locus_tag="PK634_12125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014290874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOW98525.1"
                     /translation="MKKTSSIRPCSAHRQYSRHITECAERAPSRAMLRAVGFTNDDFK
                     KSQVGVASTWSMVTPCNMHIDRLAKEASAAVNAAGGKAVIFNTITISDGISMGTEGMK
                     YSLVSREVIADSIETVAGCEQFDGLVAIGGCDKNMPGCLMAMARLNRPAVFVYGGTIL
                     PGRFRGEKVDIVSVFEAVGAHARGQISSRDLAGIEACAIPGEGSCGGMYTANTMASAI
                     EAMGMSLPNSSAQAANSTAKTRDCREAGRAVMQLIRRGIRPRDIMTREAFGNAIAVVM
                     ALGGSTNAVLHLLAMANTAGVKLALDDFTRIGRKVPVLADLKPSGSFLVADLVEIGGV
                     TPLMKTLLDAGFLRGDCLTVTGRTLRENLASVKSYPPGQTVIRPLRNPIKKEGHLVIL
                     YGNLAPEGAVAKISGYEGTRFTGKARVFESEESALARILDGTARKGDVIVIRHEGPRG
                     GPGMREMLAPTSAVMGRGLGRDVALVTDGRFSGGSHGFVVGHITPEAATGGPIAVVRN
                     GDPITIDAEKRRITLDIPGAELKRRLSRWKPPKPRYTRGVLAKYARTVTSASRGALTD
                     LPQAGGRRLTSGGGPL"
     gene            2732..2807
                     /locus_tag="PK634_12130"
     tRNA            2732..2807
                     /locus_tag="PK634_12130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:Thr,seq:cgt)
     gene            2822..3304
                     /locus_tag="PK634_12135"
     CDS             2822..3304
                     /locus_tag="PK634_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98526.1"
                     /translation="MKGKHHLVVSIAGAAGIYTASRSAPMSLSFLAFGVLLDLDHWID
                     YWIEYGARLDVPHFFRAVSRKQFRRAFLFLHAWEGFLLSALLAWWSGWPPLLSGACLG
                     WGSHLLCDQFSTKPSPWGYFLAWRIAGRFDYKHAFPVPYRPPPGDHDESDGRSPPACV
                     "
     gene            3358..4056
                     /locus_tag="PK634_12140"
     CDS             3358..4056
                     /locus_tag="PK634_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOW98527.1"
                     /translation="MAELAAYLHVTPPDVEGLVRQGGIPFERKGARVVFRRNDIDAWA
                     SQRLLGSSRENLQVFHRKSSARAHDLSRQHAIVAELVRADCIAVAMTCRTRASVIRGM
                     IKIAECAGLLYDPADLLKSIESRERLCSTAIPGGIALLHPRSPNPFFSEDSFVALGRT
                     IQSIPFGATDGSTTDLFFLVCCQEESLHLHVLARLCMMVRDTALIQELREAEGPSAMH
                     EALARAETDVIRHL"
     gene            complement(4060..4869)
                     /gene="surE"
                     /locus_tag="PK634_12145"
     CDS             complement(4060..4869)
                     /gene="surE"
                     /locus_tag="PK634_12145"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOW98528.1"
                     /translation="MAFASGIIPAMHILLSNDDGVYAPGLAALREAVKDLGDVRVVAP
                     SGEQSAVGHAITLSDPIKTKKIFRNGSFFGHAVGGTPADCVKLAACALLDHRPDLVIS
                     GINLGPNAGISVIYSGTVSAATEGTILGIPSMAISIATFKDPVWETAAHVARHLAALI
                     LKNGLPPRTLLNVNVPNLPLARIRGFAVTHMGESRFVETFDRRTDPRGNEYFWMDGEL
                     EQYGEMAGSDLQALNDGFVSLTPIWFDLTNRDALPVLKRWPLDRPAAWPVA"
     gene            complement(4895..6343)
                     /locus_tag="PK634_12150"
     CDS             complement(4895..6343)
                     /locus_tag="PK634_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98529.1"
                     /translation="MAGGCGAAESPSTPRGWSEHRDPQGFTVPLPPDWSAASDTRTGR
                     ITIRGPADEEVAIWPVFIPGPLDASAAGAVLGRLAERVQPGSRWEAPEQAGPSAVRAI
                     GRAGSQAAVAIFSWAASPRGSGGCLYIARAADGPFRQSEEIFSSIMAGFRVTGPPSDG
                     TPAKKAAVQYVRWTDPREQAFSVEVPARWKVQGGLFRFASVDVRGAIELVSPDGEVRI
                     TAGDAELPSFTEPSSMLMMTGFQEGSWYSPGYGVNMMVRRYLPGLAFAREYAESRVAR
                     GCTDVRIGESRERPDAVQAVNEVNARFGVYGIAQQLTAGEVFFTARMGGRPVEGYCFA
                     GTQQTQMSGMGLWKVEHLFTALAAVEKKDLARDVLVHLIESYQLNPQWVAMQQGITAN
                     TSKIVSQTHEEISGIIQGTYENRQRSDDEISRRRSNAMLEVEDVVDPVTGRELKIESG
                     SDYYWIDHRGTIVGTDTHTLPGLDFREMIRLP"
     gene            complement(6426..6755)
                     /locus_tag="PK634_12155"
     CDS             complement(6426..6755)
                     /locus_tag="PK634_12155"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOW98530.1"
                     /translation="MRMLVAGMAMTLALLVGCKTTECCKKSECKKSEKAETTQVDMKK
                     VESSTIDKIGYDAESQTLTVSFDNGDTYNYKKVPEKTYKALMKAKSKGKYFHKNIKDK
                     FESEKAG"
     gene            complement(6831..7361)
                     /locus_tag="PK634_12160"
     CDS             complement(6831..7361)
                     /locus_tag="PK634_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98531.1"
                     /translation="MKKSVGLCLLVLFLAVSLPRARAYENDLWGEPPSRFNPTWFVAP
                     YGAYSIGVGSEPTPDDTAGGGLAFHLDFIRLYGEFLTTLDDWTRYYYGGGADLCIVLV
                     RHDGINPYIGGGGGFYGFSNDEFENVEMAYVVEGLAGIRLGYFNFFAKYRRFVWTEAD
                     FADGWDYVQVGGGFAF"
     gene            complement(7468..>7829)
                     /locus_tag="PK634_12165"
     CDS             complement(7468..>7829)
                     /locus_tag="PK634_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98532.1"
                     /translation="EGCDGLKTGYFKLGGYSMAVTARRGDARVIAVILGSLKREVRDA
                     RASELLSQGLADLARRPPPPPPRPAVTAAAEPITAPPEEDEAAPAGRAWKWGLAGLAV
                     LAVAALAIWSRRPRFNR"
BASE COUNT         1371 a         2580 c         2517 g         1361 t
ORIGIN      
        1 gtgcgcaagg gcctgatcgg caagatcggc gagaacatgg cggtgcgccg cttcccggcc
       61 aacttgttcg ccgggttgtt cggattcgac cccaagaaga actacttcga ggccgaggaa
      121 ggcgcggaaa aagcccccac ggtcgccttt tgacccgggc gatttcccac ggacggatcg
      181 ccgaggcgcc actcggtccg tttggtcgaa tcgcggcgca gcgcttcttc cacactttgc
      241 aaagtgtgga aatggccatc agggcatggt gatcaggccg aatcctgcct ggccagcagg
      301 atgagcgttt tcgagagagg atcggagggg cgcatgcatt catacttccg gtgacggcgc
      361 cgcacgatac ctcttctctg caacttcgac aggtggcgag acagcgccgg caatgaatag
      421 ccggtcgcac caagcagttc cagaactccc atgggttttc cggccagcag tttgacgagt
      481 tgcacacgcc tcggatgggt gaaggccgtg agcgcctgca taaacacgcc tgctgtattc
      541 tgcttgaccc gaagggattg catgacagaa cccaaaaggg ctgccgcgtg cggcagggag
      601 gaatcggcgc ccactgtata atacacgaat cgcttcttcc gcatgaccgt cagcaagccg
      661 cgagcgttga gcgacctcaa gtactgactg gcggtcgcca tagagatgtc catttcccgt
      721 gccacgtccg aaacggtcag gggctctcgg gcgatgagca ggcgaaggat gtccaaccgg
      781 cgctcattgg ccagggcccg gcaggttctc cacaacgtgg gatgcagtcc ggataacgtg
      841 ttatatttca tgatcacact ttgcaaagtg tggaaatagt cgtcaagcaa agaagcgcat
      901 cagcgctccc gctctttgta tttcgatggg gaaggatgta tactggataa aaaagatcct
      961 tatgaaaaag acgtcatcga taagaccatg ctccgcgcac cggcagtact cccggcatat
     1021 aacggaatgc gcagagcgcg cgcccagccg ggcgatgctc cgcgccgtcg ggttcacgaa
     1081 cgacgatttc aagaaatccc aggtgggcgt cgcctcgacc tggagcatgg tcacgccctg
     1141 taacatgcac atcgaccgcc tcgcgaaaga agcctcagcc gctgtgaatg ccgccggcgg
     1201 caaggccgtg atcttcaata cgattacgat ttccgacggg atatcgatgg gcacggaggg
     1261 aatgaagtat tcgctcgtgt cgcgcgaagt catcgctgat tccatcgaga ccgtcgccgg
     1321 ttgcgagcag tttgacggcc tggtggccat cggcggttgc gacaagaaca tgcccggctg
     1381 cctgatggcc atggccagac tgaaccgacc ggccgtgttt gtgtacggcg gcaccatact
     1441 gcccggacgg ttccgcgggg agaaggtcga cattgtttcc gtgttcgagg ccgttggcgc
     1501 gcatgcgcga ggacaaatat catccaggga tctggcaggc attgaggcgt gcgcaatccc
     1561 cggcgaaggg tcctgcggag ggatgtacac ggctaatacc atggccagcg ccatcgaagc
     1621 catgggcatg agcctcccca acagttccgc ccaggcggcc aactcgacgg ccaagacgag
     1681 ggattgccgc gaggcgggca gggccgtgat gcaactcatc cgccgcggca tccgcccccg
     1741 cgacatcatg acccgcgagg ccttcgggaa cgcgatcgcc gtggtcatgg ccctcggcgg
     1801 ctctaccaac gccgtgctgc acctgctggc catggccaac accgccggcg tcaaacttgc
     1861 actcgacgat ttcacgcgga tcggtcggaa ggtgccggtt cttgcggatc tcaagcccag
     1921 cggatcgttt ctcgtggccg acctggtcga gatcggcggg gtgacgcccc tgatgaagac
     1981 gctgctggac gccggcttcc tgcgcggcga ctgcctcacg gtcaccggcc gcaccctccg
     2041 ggagaacctg gcgagcgtga aatcctaccc gcccggccag acggtgatcc gtcctctgcg
     2101 aaatcccatc aagaaggaag gacacctggt catcctctac ggcaacctgg caccggaagg
     2161 cgccgtggct aaaatatccg gatacgaggg gacccgcttc acggggaaag cccgggtatt
     2221 tgaatccgag gaatcagcgc tggcccgcat cctggatggc acggcgcgaa agggcgatgt
     2281 gatcgtgatc cggcacgagg ggccccgcgg cggtccgggc atgcgcgaga tgctggcgcc
     2341 gacctccgcg gtaatggggc gcggcttggg ccgcgacgtg gccctggtca ccgacgggcg
     2401 tttttcgggc ggcagccacg gctttgtcgt cgggcatatc accccggagg ccgccaccgg
     2461 cggaccgatc gccgtggtcc ggaacgggga cccgatcacg atcgacgcag agaagcggcg
     2521 catcacgctg gatattcccg gcgccgaact gaagcggcgg ctgtcgcgat ggaagccccc
     2581 gaagccccgc tatacccgcg gtgtgctggc caagtacgcg cgcacggtaa cgtcggcctc
     2641 ccggggcgcc ctgaccgatc ttccgcaggc cggcgggcgg cgcttgactt cgggaggggg
     2701 gccgctatag tcgccgctca tcgacgcgta agccaacgta gctcagctgg cagagcaggt
     2761 cattcgtaat gatcaggtcg tcggttcgaa tccgaccgtt ggctccaatc cacgcgagcc
     2821 catgaaaggc aagcaccacc tggtcgtttc catcgcggga gccgccggga tatacaccgc
     2881 ctcccgatcc gcgccgatga gcctgtcctt cctagccttc ggagtccttc tggatctcga
     2941 ccattggatc gattactgga tcgaatacgg ggcgcggctg gacgtgcccc atttcttccg
     3001 ggcggtttcc cgcaagcagt tccgtcgggc tttcctgttc ctgcacgcct gggaaggatt
     3061 ccttctctcg gcgctgctgg cgtggtggag cggttggccc ccgctgctgt ccggcgcatg
     3121 cctggggtgg ggcagccatc tgctctgcga ccagttcagc acgaagccca gcccgtgggg
     3181 ctacttcctg gcctggcgca tcgcgggccg tttcgactac aagcacgcct tcccggtccc
     3241 gtaccgcccg ccgccgggcg accacgacga aagcgatgga cgctctccgc cggcttgcgt
     3301 atagtgcggg ccgatatcag ccgcgcggga gaacagatgc cccatcgtga aatgacaatg
     3361 gcggaactcg ccgcctacct gcacgtgacc ccacccgacg tggaggggct ggtccggcag
     3421 ggaggcatcc cgttcgagcg caagggggct cgcgtcgtct ttcgccggaa cgacatcgat
     3481 gcgtgggcct cccagcggct gttgggctcg agccgcgaaa acctgcaggt gttccatcgg
     3541 aagagctcgg ccagggcgca cgacctttcc cggcagcacg cgatcgtcgc cgagttggtc
     3601 cgcgccgatt gcatcgcggt ggcgatgacc tgccggaccc gggcctcggt gatccggggg
     3661 atgatcaaga tcgcggagtg cgcgggcctg ctctatgacc cggccgacct gctcaagagc
     3721 atcgaatccc gcgaacgcct gtgctcgacg gccatccccg gcggcatcgc cctgctgcat
     3781 ccccggagcc ccaacccgtt tttctcggag gattccttcg tggcgctggg ccgcacgatc
     3841 cagagcattc ctttcggcgc cacggacggc tccacgacgg atctcttctt cctggtctgc
     3901 tgccaggagg aaagcctgca cctgcacgtg ctggcccgcc tctgcatgat ggtccgggac
     3961 accgcgttga ttcaggaact gcgagaggcc gaggggcctt cggcgatgca cgaggcgctg
     4021 gcgcgggcgg agacggacgt catccgccac ctgtagttat cacgccaccg gccaggcggc
     4081 gggccgatcg agcggccagc gcttcaacac cggcagcgcg tcccggttcg tcaggtcgaa
     4141 ccagatcggg gtcagggaaa cgaacccgtc gttcaaggcc tggagatcgc tgcccgccat
     4201 ctcgccgtac tgctccagtt caccgtccat ccagaagtac tcgttgcccc ggggatcggt
     4261 gcgccggtcg aacgtctcca cgaaccgcga ttcgcccatg tgggtcacgg cgaagccgcg
     4321 gatccgggcc aggggaaggt tggggacgtt gacgttgagc agcgtgcgcg gcggcaatcc
     4381 gttcttcagg atgagcgccg ccaggtgccg ggccacgtgc gccgcggtct cccagaccgg
     4441 atccttgaag gtggcgatcg agatggccat gctggggatg cccaggatgg tgccctccgt
     4501 ggccgccgaa acggtgcccg aatagatgac gctgatcccc gcgttcggcc cgaggttgat
     4561 gccgctgatc acaaggtcgg gccggtggtc cagcagggcg caggcggcca gtttcacgca
     4621 atccgcgggc gtcccgccga ccgcgtggcc gaaaaaggat ccgttccgga atatcttctt
     4681 ggtcttgatc gggtcggaaa gcgtgatggc gtgcccgacg gcgctctgtt cgccgctggg
     4741 cgccaccacg cgcacatcgc ccagatcctt cacggcctcg cgcagcgccg ccaggcccgg
     4801 ggcgtagaca ccgtcgtcgt tgctcaacag gatatgcatg gccggtatga tgcccgacgc
     4861 gaacgccaag acaagctgca aaatgcgcgc cgggtcaggg caggcggatc atctcgcgga
     4921 agtccaggcc gggcagcgta tgggtgtccg tgccgacgat cgtgccgcgg tgatcgatcc
     4981 agtaatagtc tgagccgctc tcgatcttca actcgcgccc tgtgaccggg tcgaccacgt
     5041 cctcgacctc cagcatggcg ttcgagcggc ggcggctgat ctcgtcgtcg ctccgctgcc
     5101 ggttctcgta ggtgccctgg atgatgccgg atatttcctc gtgcgtctgg ctgacgatct
     5161 tgctggtgtt ggccgtgatg ccctgctgca tggccaccca ctggggattc agctggtacg
     5221 attcgatcag gtgcacgagg acgtcgcggg ccaggtcctt tttctccacg gcggccagcg
     5281 ccgtgaagag gtgctcgact ttccacaggc ccatcccgct catctgggtc tgctgcgtgc
     5341 ccgcgaagca ataaccctcc acggggcggc cgcccatgcg ggccgtgaaa aagacctcgc
     5401 cggccgtgag ttgctgcgca atgccgtata cgccgaagcg ggcattgacc tcgttcaccg
     5461 cctgcaccgc atccgggcgc tcccggcttt ccccgatccg gacatccgtg cagccgcggg
     5521 ccacccggga ctccgcgtat tcccgcgcga aggcaaggcc ggggaggtag cggcgcacca
     5581 tcatgttcac cccgtagccc ggcgagtacc aggagccctc ctggaacccg gtcatcatca
     5641 acatggacga gggttcggtg aaactgggca gttccgcgtc gccggccgtg atgcgcacct
     5701 cgccgtcggg cgaaaccaac tcgatggcgc cccggacgtc caccgacgcg aaacggaaaa
     5761 ggccgccctg gactttccat ctcgcgggca cctcgacgct gaaggcctgc tcccggggat
     5821 cggtccagcg cacgtactga accgcggcct tcttcgccgg ggtcccgtcc gacggaggcc
     5881 cggtcacccg gaaccccgcc atgatgctgg aaaaaatttc ttcggattgg cggaatgggc
     5941 cgtccgccgc ccgggcgatg tacaggcagc cccccgagcc gcgtgggctg gccgcccaac
     6001 tgaaaatggc gacggccgct tgcgagcccg cgcggccgat cgcgcgaacg gccgacggcc
     6061 cggcctgctc cggggcctcc cagcgactgc cgggctgcac gcgctccgcc agacggccga
     6121 ggacggcccc ggccgcggag gcatccagcg ggccggggat gaacaccggc cagatcgcca
     6181 cttcctcgtc cgccgggccg cggatcgtga tgcgaccggt gcgcgtgtcc gacgcggcgc
     6241 tccagtcggg gggcaggggg acggtgaatc cctgggggtc gcggtgctcg ctccagcccc
     6301 gcggcgtgga aggcgactcc gccgcgccgc agccgcccgc caagaaaaga gccgccgcgg
     6361 cccacgccgc agcggctctc caagcgcatg tccggcggtt catgtgccgg tggatggggt
     6421 tcgtgtcagc ccgctttctc ggactcgaac ttgtccttga tgttcttgtg gaagtacttg
     6481 cccttggact tggctttcat cagggccttg taggtcttct ccggcacctt cttgtagttg
     6541 taggtgtcgc cgttgtcgaa gctgacggtc aacgtctggc tctccgcgtc gtacccgatc
     6601 ttgtcgatcg tggaggattc caccttcttc atatcgacct gggtcgtctc ggccttttcg
     6661 gacttcttac actcggactt cttgcagcac tccgtcgtct tgcagccgac cagcagcgcc
     6721 aacgtcatcg ccatgcctgc cacgagcatt ctcatcgttg tctcctttgc tgatgtcaaa
     6781 ctgcgggaaa tcgtagtggc cggcgcgtgc gccgtcaatg cccgtttggg ctagaaggcg
     6841 aacccgcccc ccacctggac gtaatcccag ccgtccgcga agtccgcctc ggtccagacg
     6901 aagcgccggt acttcgcaaa aaagttgaag tagcccaacc ggatgccggc cagcccctcc
     6961 acgacgtacg ccatctcgac attctcgaat tcgtcattgc tgaatccgta aaaaccaccg
     7021 cccccgccga tgtacggatt gatgccgtcg tgccgaacca gcacgatgca aagatcggcc
     7081 ccacccccat agtagtacct tgtccaatca tcgagcgtgg tcaggaactc gccgtacagc
     7141 cggataaaat ccaggtggaa cgccagcccg ccgccggccg tgtcgtccgg agtcggctcg
     7201 ctgccgacgc cgatggagta cgcaccgtag ggcgcgacga accaggtggg attgaaccgc
     7261 gagggcggct caccccataa atcgttctcg taggcgcgcg cgcgcggaag gctgacggca
     7321 agaaacagaa cgagcaggca cagtccaacg ctcttcttca taacgggcct cctttcccga
     7381 atcggcgcat gcacgaccgc tccaagagca ttctacccgc gggcaagtcc ctgacaatca
     7441 ccttgtgccg tcgcgcatcg gcccgcctca gcggttgaat cgcggtcgcc ggctccagat
     7501 cgccagggcg gcgacggcca ggacggcaag gcccgccagg ccccacttcc aggcgcggcc
     7561 ggcgggagca gcctcatcct cctctggagg agcagtgatc ggttccgcgg cggcggtcac
     7621 ggccgggcgg gggggagggg gcgggggccg gcgcgccagg tcggccagtc cctgcgaaag
     7681 aagttcgctg gccctggcat cacgcacttc gcgtttcaag ctgccaagga tcaccgcgat
     7741 gacccgggcg tcaccgcgcc gggcggtgac cgccatggag tagccgccca gcttgaagta
     7801 gcccgtcttg agcccgtcac acccctcga
//
LOCUS       DAOOTK010000132         7756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5879_length_7756_cov_3.611089, whole genome shotgun sequence.
ACCESSION   DAOOTK010000132 DAOOTK010000000
VERSION     DAOOTK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_7756_cov_3.611089"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK634_12170"
     CDS             complement(<1..250)
                     /locus_tag="PK634_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOW98533.1"
                     /translation="MQDFEKLGAFYLGKVFDAAAGSVRDELLLYDSRDLTTHAVVLGM
                     TGSGKTGLCLALLEEAAIDGVPAIAIDPKGDLGNLLLAF"
     gene            complement(315..1703)
                     /locus_tag="PK634_12175"
     CDS             complement(315..1703)
                     /locus_tag="PK634_12175"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOW98534.1"
                     /translation="MRTLWKILKIIAIVIVLLLVGLRLALSPLVKTVANKVLPEALGT
                     EASLGDVSFGFLRGTTGLGDLRIGQPKGFDTEDKDLFRFSSFEAKVRPGTLKSGEVEV
                     ERLELKDLYVHVIRNKDGVLNVAQLGAKEKAAEPEVKAPVEKKEEEARPLRVKLLAVR
                     NGTIKYTDYGLGENPVRVNVTDIDFEVKDLLLDAKADASVVEPAIVSLVARLKQEGVP
                     DGRLGLVARVGPVGEAIPPVNASARLVGFDLTPFKPLVPPATLTLLGGMGLDLTADVG
                     LAPDLLEVGGEVVMAGGSTFPFRVGGTPEKPEFDTSSVLFGTMGRVSGAVSGTAKDVA
                     ETGKAAAGAVVEGAGALAGGAFKAIGGLGRAVKDTAEAGIKGDLKDAGSALVGGVSEA
                     AGEASESVKDTASSAKEGLEASAEALSGADALQAWRDATDTRWDAAWAGAQERVATAP
                     FPPAREAAPSAP"
     gene            complement(1731..2279)
                     /locus_tag="PK634_12180"
     CDS             complement(1731..2279)
                     /locus_tag="PK634_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HOW98535.1"
                     /translation="MNLSDHAKPSRNPADRAARKRRRRRRALIVAGLFMAFGVVVAIG
                     ERARAPKAGTAGTAVERATPPSAESGDAAEAQAPAARKPALPATFRLGAGYAGGFFLG
                     WLFRKFIKTALLATGALVGLAALARGLGWFDLDWTTAGTYVRESFAWLGGHAGSFKDF
                     IMGYVPSAGAAALGFFFGLWSD"
     gene            complement(2328..2756)
                     /locus_tag="PK634_12185"
     CDS             complement(2328..2756)
                     /locus_tag="PK634_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98536.1"
                     /translation="MTKRSAVRHAFLALFVLCLPAAAESMLALKVSSPLMVSGAAGLR
                     FPSAGRTWSPSIQAEAGVGGGKILVGGDTFGAGFGLGVKAVLLRTWLEPVEVEPDTTY
                     LGAEFEAGFRQLFAAAGAYARVGGEDNDDLLLGISLGWML"
     gene            complement(2769..3449)
                     /locus_tag="PK634_12190"
     CDS             complement(2769..3449)
                     /locus_tag="PK634_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98537.1"
                     /translation="MKAPSSIRAACLAAILLGANARAGEIGERLWYADNGQQHAWVAG
                     GALEGERPPGTNRVAFGLLDLEYGRYDNEGDTETHGRYAGALGIALPHGWRAGAGFEY
                     RDIRTSLKEGWRWDDDHPEERERNADIYGPMLYAAWEGFVRNPRAGWKLELSWMCYDL
                     GGLQDIGYDGSNVRMAASAFYRFRRLTLRTGYRLEEFRDMPDRVANDSASSRDTIQGP
                     FFALGVSF"
     gene            complement(3454..4218)
                     /locus_tag="PK634_12195"
     CDS             complement(3454..4218)
                     /locus_tag="PK634_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98538.1"
                     /translation="MRGRLFWTMGSFLCWAGLAGGAGPSYTAAPALQYFEWKEDAFRL
                     EEKGLLPSLLLEREAAGGEHWGGRWSLLAFRGEVEYDGLKQDFTPYRSKTLYVGGDLR
                     GTVHGRWKAAESIEIQPLAGLGSRLWKRRLDNTDEMTSGYDEDWWNVYLLAGARAVWK
                     TRSGATTTLEGGLRQSLYTYEKVNFEVIGRSSVPTLTPSNRASGYAALAWARGDYVLK
                     LFYEGMRFGHSETDRSGFYQPESEALVLGGSIGVQW"
     gene            4329..5405
                     /gene="rlmN"
                     /locus_tag="PK634_12200"
     CDS             4329..5405
                     /gene="rlmN"
                     /locus_tag="PK634_12200"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOW98539.1"
                     /translation="MKDRAPAIHDMSASEIEALLREWRQPAYRARQIYRQLYAQLAAA
                     PGEMSDLPAALRERLAQEVRFNELELDRVQTGDGGLTRKALFRVNGGPPVEAVLMIYR
                     DRATVCVSTQSGCAMACRFCATGRMGFRLNLTAGQMVEQVLWAAREARAAPAPGKPLL
                     TNVVYMGMGEPFNNYDRWWASVERLHDPEGFNLGARNFTVSTVGIVPGILRLAREKLP
                     VNLALSLHAPDDELRSQLVPVNRKYPVAEVIEAVREYTDKTSRRVSFEYVLLQGVNDQ
                     PARAQALARLLKGTATRPGVRLCHVNLIPWNPVPGEPLKPSERQRVLDFQAALERHRI
                     ACTVRVQRGVDIAAACGQLAGGVQ"
     gene            complement(5417..6178)
                     /gene="tatC"
                     /locus_tag="PK634_12205"
     CDS             complement(5417..6178)
                     /gene="tatC"
                     /locus_tag="PK634_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOW98540.1"
                     /translation="MSMPDDADSSGVKPFLEHLEDLRWTVVRCAVALLVGVCIAYPFA
                     PNVLRLLSRPLSSVTDAPDQFLRSLEVGGAFSVSLRLAGWTGLLLSSPFLVLFIGRFV
                     FPGLFEHERRTVARMFGFAVVLFAGGLILGYAYALPVAVKMMFALHGWMGVRAEWTIT
                     NYLAFSVPLLIGFGLVFELPVVLVALGRMGFITAAQLRQRRRHAIVAALVIGMVMTPP
                     DVVSQVLMATPLIGLYEVSIWLVALGERRAKSVPA"
     gene            complement(6162..6449)
                     /locus_tag="PK634_12210"
     CDS             complement(6162..6449)
                     /locus_tag="PK634_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOW98541.1"
                     /translation="MTGFETTAFLAGNTGGGEILLVFVVALVLFGSKRLPGLARSLGR
                     AIEEFRRAARDVSDEIQRASDEPPAPPPASDQSALPPLPPPTKDRDHEHAG"
     gene            complement(6534..7046)
                     /locus_tag="PK634_12215"
     CDS             complement(6534..7046)
                     /locus_tag="PK634_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOW98542.1"
                     /translation="MRGWVVVAVAGLLVVSSGCKKRPKPGQAGVDNVGGVVGSDLYGE
                     GADMLGDRFAGGEEVRGQFTAVYFDYDSSQIKDSERSKIDAVASHLQSAGTANLVVEG
                     NADERGSNEYNLALGERRALAIRAYLVGLGIDGNRITTKSLGEEQPVVMGHDEESWSQ
                     NRRGEFVLIQ"
     gene            complement(7103..7672)
                     /locus_tag="PK634_12220"
     CDS             complement(7103..7672)
                     /locus_tag="PK634_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98543.1"
                     /translation="MASFPGLNTGGAVSPGGREMALILSRTGNPELFVKGGGGLTKLT
                     PTTQAAEASPAWSPDGSRIVYVSDQAGTPQLYMISRSGGRAQRLTSRGSENVAPDWGA
                     NGWIAFSSKLGGRYQICIMNPDTQEIRQLTADGTDYEDPAWAPDGRHIACARTAGYRS
                     SIYLVDTMGDPPIPLLDASGDWFSPSWAP"
BASE COUNT         1256 a         2758 c         2548 g         1194 t
ORIGIN      
        1 ggaaggccag gagcaggttg ccgaggtcgc ccttcggatc gatggcgatg gcgggcacgc
       61 cgtcgatcgc cgcctcttcg agcagggcga ggcagaggcc cgtctttccg ctgccggtca
      121 tgcccagcac gacggcatgg gtggtcaggt cgcgggaatc gtagagcagg agttcgtcgc
      181 gcaccgaacc ggccgcggca tcgaagacct tgcccaggta aaacgcgccc agcttctcga
      241 agtcctgcat gatgccctcc cgcgttattc gtcgcggggc atcatacatc gacgcgtcac
      301 gcttgtcatc cggttcaggg ggcggacggg gccgcttcgc gagcgggcgg gaacggcgcc
      361 gtcgccaccc gctcctgcgc gccggcccag gcggcgtccc atcgcgtatc ggtcgcgtca
      421 cgccaggcct ggagcgcgtc cgcgccggaa agcgcctccg cggaggcctc cagcccttcc
      481 ttcgcggaac tcgcggtgtc cttcaccgac tccgaagcct cgccagccgc ctcgctcacg
      541 ccgcccacca gggcgctgcc ggcgtccttc agatccccct tgatgcctgc ttccgcagta
      601 tccttcaccg ctcggccgag ccctccgatg gctttgaacg ccccgcctgc cagcgcgccg
      661 gctccttcca ccacggcgcc cgcggcggcc ttgcctgtct cggcaacgtc cttggccgtg
      721 cccgagaccg ccccgctcac gcggcccatc gttccaaaga gcacgctcga ggtgtcgaat
      781 tccggcttct ccggcgtgcc gcccacgcgg aaggggaatg tcgagccgcc ggccatgacg
      841 acctcgccgc cgacctcgag gaggtccggc gccaatccga cgtccgctgt gaggtcgaga
      901 cccatgccgc ccagcagggt cagggtggcc ggcggcacga ggggtttgaa cggggtcagg
      961 tcaaaaccga ccaggcgcgc ggaggcattg accgggggaa tggcctcgcc caccgggccc
     1021 acacgggcga ccaggccgag gcggccgtcg ggcacgcctt cctgtttcag gcgcgccacc
     1081 agggagacga tggccggctc cacgacggag gcgtcggcct tcgcgtccag cagcaggtct
     1141 ttcacctcga agtcgatgtc ggtcacgttg acccgaacgg gattctcgcc caggccgtag
     1201 tcggtgtact tgatcgtgcc gttgcgcacg gccagcagct tgacgcgcag cggccgggcc
     1261 tcctcctcct tcttctccac gggcgccttg acctccggct cggcggcttt ttccttcgcg
     1321 ccgagttgcg ccacgttcag cacgccatcc ttgttgcgga tcacatgcac gtacaggtcc
     1381 ttgagttcca gtcgctccac ctcgacctca cctgacttca gcgtgcccgg ccggaccttg
     1441 gcctcgaagc tcgagaagcg gaacagatcc ttgtcttccg tgtcgaaacc cttcggctgc
     1501 ccgatgcgca ggtcgcccag acccgtggtc ccgcgcagga acccgaagct cacgtcgccc
     1561 agcgaggcct ccgtccccag ggcctcgggc agcaccttgt tggccaccgt tttcaccagc
     1621 ggggaaagag ccagccgcaa cccgaccagc agcagcacga tcacgatcgc aatgattttc
     1681 aggattttcc atagtgttcg catgatggcc ttcctttctg gttccgcggt tcagtcgctc
     1741 cacaagccaa agaaaaatcc gagggctgcc gcgccggcgg aggggacata gcccatgatg
     1801 aaatccttga acgacccggc gtggccgccg agccaggcga aactctcccg gacgtaggtt
     1861 cccgccgtcg tccaatccag gtcaaaccag ccgagcccgc gcgccagcgc ggccagaccg
     1921 accagcgccc cggtcgccag cagcgccgtc ttgatgaact tccggaaaag ccagcccagg
     1981 aagaacccgc cggcataccc ggcgcccaag cggaatgtcg ccggcagggc gggcttccgg
     2041 gcggccgggg cctgcgcctc cgccgcgtcg ccggattccg cggacggcgg cgtggcgcgc
     2101 tcaaccgcgg ttcccgccgt cccggctttc ggggcccgcg ccctctcgcc gatggccacg
     2161 acgacgccga aggccatgaa cagcccggcg acgatcaggg cgcgtctacg gcggcggcgc
     2221 ttcctcgccg cccggtcggc cgggttgcgc gacggctttg catggtcgga gagattcatg
     2281 tggcctgcac tataccacgg attgggtccg ggaatacagg aacgcgttca cagcatccac
     2341 ccgaggctga tgccgaggag aaggtcgtcg ttgtcctcgc cccccacccg cgcatacgcg
     2401 cccgcggccg cgaacaactg gcggaagccc gcctcgaatt ccgcgccgag gtaggtcgtg
     2461 tccggttcca cctcgaccgg ctccagccag gttcgcagga ggacggcctt gacgccaagc
     2521 ccgaaccccg cgccgaaggt gtcgccgccg accaggatct ttcctccgcc gaccccggcc
     2581 tcggcctgga tcgacgggct ccacgttcgg ccggcggacg ggaaccggag ccccgccgcg
     2641 ccgctgacca tcagcgggga ggagaccttc agggccagca tcgactccgc cgcggcgggc
     2701 aggcacaaaa caaacaacgc cagaaacgca tgcctcacgg cagatcgctt ggtcatgggt
     2761 aatctccttt agaagctgac cccgagcgcg aaaaaggggc cctggatggt gtcgcgggac
     2821 gaggcgctgt cgttggccac gcggtcgggc atatcgcgga attcctccag gcggtaaccg
     2881 gtgcggagcg tcaggcgccg gaagcggtag aaggccgagg cggccatgcg cacattggag
     2941 ccgtcgtacc cgatgtcctg caggccgccc aggtcgtagc acatccagga gagttccagt
     3001 ttccagccgg cgcgggggtt tcggacgaag ccttcccagg cggcatacag catcgggccg
     3061 tagatatccg cgttgcgctc ccgctcctcg ggatgatcgt cgtcccaccg ccagccctct
     3121 ttcaggctcg tgcggatgtc gcggtactcg aatccggcgc ccgcccgcca gccgtgcggc
     3181 agcgcgattc ccagcgcgcc cgcgtaccgg ccgtgggtct cggtgtcccc ctcgttgtcg
     3241 tagcgtccgt actcgaggtc gaggagcccg aacgccaccc ggtttgtgcc cgggggccgc
     3301 tcgccttcca gggcgccgcc ggccacccag gcgtgctgct ggccgttgtc ggcataccag
     3361 agccgctccc cgatttcgcc ggcgcgggcg ttcgcgccga ggaggatcgc ggcgaggcac
     3421 gccgcgcgga tggacgaagg cgccttcatg cgttcaccat tgcaccccga tgctgccgcc
     3481 caggaccagg gcctcggatt cgggctggta gaacccggag cggtcggtct cggagtgccc
     3541 gaatcgcatc ccctcgtaaa agagcttcag cacataatcg ccccgggccc acgccagcgc
     3601 ggcgtagccg gacgcgcggt tggagggagt cagggtcggg acggaggagc gcccgatgac
     3661 ctcgaaattg accttctcgt aggtgtacag ggactgccgg aggccgccct cgagcgtcgt
     3721 cgtggcgccc gacctggttt tccagacggc gcgggcgccc gccagcaggt agacgttcca
     3781 ccagtcctcg tcgtagccgc tcgtcatctc gtcggtattg tccaggcgcc gcttccagag
     3841 ccggctcccc aggcccgcca gcggctggat ctcgatgctt tcggcggcct tccaccggcc
     3901 atgcaccgtg ccgcggaggt cgccgccgac gtacagcgtc ttgctccggt acggggtgaa
     3961 atcctgtttg agcccgtcgt attcgacttc cccgcgaaag gccaggagac tccaacgccc
     4021 gccccagtgt tcgccgcccg cggcctcgcg ttcgaggagc agagaaggca gcaggccctt
     4081 ttcctcgagg cgaaatgcgt cttccttcca ttcgaaatac tggagcgcgg gcgccgcggt
     4141 gtacgagggc ccggcgcctc ccgccaggcc cgcccagcac aagaaagatc ccattgtcca
     4201 gaaaagcctg ccgcgcatgg tcgattcccg cggcggagtg tagccgaagc cgtccgcgcg
     4261 cttcaactgc caaacggcgg cccgggagcg agaaagacag gcgcccttcc ttgtggtagg
     4321 ctggccgcat gaaggacagg gccccggcca ttcacgacat gtccgcgtcc gagatcgagg
     4381 cgctcctgcg ggagtggcgg cagccggcct accgcgcccg gcagatctac cggcagctct
     4441 acgcccaact ggccgccgcg ccgggggaga tgtcggacct gcccgcggcg ctccgcgaac
     4501 ggctggcgca ggaggtccgc ttcaacgaac tggaactgga ccgcgttcag accggagacg
     4561 gggggctgac ccgcaaggcg ctcttccgcg tgaacggcgg cccgcccgtg gaagccgtcc
     4621 tgatgatcta ccgcgaccgc gcgacggtct gcgtctcgac gcagtcgggc tgcgcgatgg
     4681 cctgccgctt ctgcgcgacc gggcgtatgg gtttccgatt gaacctgacg gcgggccaga
     4741 tggtcgagca ggttttatgg gccgcgcgcg aagcaagggc cgcgccggcg cccgggaagc
     4801 cccttctcac caacgtggtc tacatgggca tgggcgagcc gttcaacaac tacgaccgct
     4861 ggtgggcctc ggtggaacgg ctgcatgatc cggaaggatt caatctcggg gcgcggaact
     4921 tcaccgtgtc cacggtcggc atcgtgcccg gcatcctccg gttggcccgc gaaaagctgc
     4981 cggtcaacct ggccctttcg ctgcatgcgc ccgacgacga actgcggtcg caactggtcc
     5041 ccgtcaaccg caagtatccc gtcgcggagg tgatcgaggc cgtccgcgag tacacggaca
     5101 aaacgagccg ccgcgtgtcg ttcgaatacg tgctgctcca gggcgtgaac gaccagccgg
     5161 cgcgggccca ggcgctggcc cgcctgctca agggcacggc gacccggccg ggcgtccggc
     5221 tgtgccacgt gaacctgatc ccgtggaacc cggttcccgg cgagccgctg aagccgtccg
     5281 agcgtcagcg cgtgctggat ttccaggcgg cgctggaacg ccaccggatc gcctgcacgg
     5341 tccgcgtcca gcgcggggtg gacatcgccg ccgcgtgcgg gcaactggcc ggcggcgtgc
     5401 agtaggcctg atgccatcag gccggcacgg atttcgcgcg gcgttccccg agcgccacga
     5461 gccagatgct gacctcgtag agcccgatga gcggcgtcgc catcagcacc tgcgacacca
     5521 cgtccggcgg ggtcatcacc atgccgatga ccagggcggc gacgatcgcg tgacgccggc
     5581 gctgcctcaa ctgcgcggcc gtgatgaagc ccatccggcc gagcgcgacc agcacgaccg
     5641 gcagttcgaa caccaggccg aacccgatca gcaggggcac ggaaaacgcc aggtagttgg
     5701 tgatcgtcca ttccgcccgc acgcccatcc acccgtgcag cgcaaacatc atcttcaccg
     5761 ccacggggag cgcgtaggca taccccagga tcaggccgcc ggcgaataga acgaccgcga
     5821 agccgaacat cctcgccacg gtccgccgct cgtgctcgaa cagcccgggg aagacaaacc
     5881 ggccgatgaa caggacgagg aatggactgc tcaagagaag gcccgtccac ccggccaggc
     5941 gcaggctcac ggaaaacgcg ccgccgactt cgagcgagcg caggaactgg tccggcgcgt
     6001 ccgtcacgga cgatagcggg cggctcaaca ggcgcaacac gttgggcgca aagggatacg
     6061 cgatgcacac cccaaccagc agcgcgaccg cgcaccggac cacggtccag cggaggtcct
     6121 ccagatgctc caggaaaggc ttcacgcccg acgaatcggc gtcatccggc atgctcatga
     6181 tcccggtcct tcgtcggcgg cggaagcggt ggcaaggcgg attgatcgga agcgggcggc
     6241 ggcgccggcg gttcatcgga ggcgcgctgg atctcgtcgg agacatcgcg agccgcccga
     6301 cggaattcct cgatggcccg gcccaggctg cgcgccaggc cgggcagccg cttcgatccg
     6361 aagagcacga gggccacgac gaagacgagc aggatttccc cgccgcccgt gttgccggcc
     6421 aggaaggctg ttgtctcgaa ccccgtcatc cggtcgcgcc ccccaattcc cttctaaagc
     6481 gcaaaagccc cggcggcaac cggggctttc gcgtcgtgac caagcgggtc gcgctattgg
     6541 atcagcacga actccccgcg ccggttctgg ctccaggact cctcgtcgtg gcccatgacc
     6601 accggctgtt cttcgccgag gctcttcgtt gtgatccggt tgccgtcgat gccgagaccg
     6661 accaggtagg cccggatggc caaggcgcgg cgttcgccga gcgccaggtt gtactcgttg
     6721 ctgccgcgct cgtcggcgtt gccctcgacg accaggttgg ccgttccggc ggactgaagg
     6781 tggctcgcca ccgcgtcaat cttggatcgc tcggaatcct tgatctggga gctgtcgtag
     6841 tcgaaataga cggccgtgaa ctgcccgcgc acttcctcgc cgccggcgaa gcggtcgccc
     6901 agcatgtccg cgccttcgcc gtacaggtcg ctgcccacca cgccgccgac gttatcgacg
     6961 ccggcctgcc cgggtttcgg gcgcttcttg catccgctcg acacgaccaa cagccccgcc
     7021 accgccacca ccacccaccc tcgcatctgc atcttcttat ctcctgtttc ctttagtttt
     7081 ctgaaaaaac aaggttccgt gctcaaggcg cccacgatgg agaaaaccaa tccccggaag
     7141 cgtcgagtaa aggtatgggg ggatcgccca ttgtgtcaac aagatagatg gaggaccggt
     7201 agcccgccgt ccgcgcgcag gcgatatgcc ggccgtcggg cgcccaggcg gggtcctcgt
     7261 aatccgtgcc gtcggcggtg agctgtcgga tttcctgggt gtccgggttc atgatgcaga
     7321 tctggtagcg cccgcccagc ttggaggaga acgcgatcca gccgttggcg ccccagtcgg
     7381 gggcgacatt ttccgagccg cggctggtca gccgctgggc gcggccgccg gaccgggaga
     7441 tcatgtagag ctgcggggtg cccgcctggt cggacacgta cacgatccgg cttccgtcgg
     7501 gcgaccaggc cgggctggcc tcggcggcct gcgtcgtggg cgtcagcttg gtgaggccgc
     7561 ccccgccctt cacgaacagc tccgggttgc ccgtccgcga gaggatcagc gccatctccc
     7621 gtccgcccgg ggagaccgcc ccgccggtgt tcagcccggg gaaactggcc acgacgcggc
     7681 gcgagccgct ccccgatctg ctcgagcgtc aggccggtca gcgggagcac gaaccagcgc
     7741 atcacggtcg cgaagg
//
LOCUS       DAOOTK010000133         7733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5897_length_7733_cov_4.806851, whole genome shotgun sequence.
ACCESSION   DAOOTK010000133 DAOOTK010000000
VERSION     DAOOTK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_7733_cov_4.806851"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK634_12225"
     CDS             <1..208
                     /locus_tag="PK634_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98544.1"
                     /translation="PPPWIRGDDILALGIPQGPRVGYWHKKAYEAQLESRFPDREALL
                     NWIREEIREQPGGGVPAGPTPGQS"
     gene            complement(189..1310)
                     /locus_tag="PK634_12230"
     CDS             complement(189..1310)
                     /locus_tag="PK634_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOW98545.1"
                     /translation="MKGSFSIGRLFGVDIRIHWTFFLLAGWFAWWGGEGDGWRAAAWA
                     LSLLAGLFACVVLHELGHSLVAIRFGGQVQAITLLPIGGVALMRKIPERPLHEFLVAV
                     AGPAVNVALFLGLALIRGGFPSWIAAEPPAGAWQEWLDTLMQANAVMAVFNLIPAFPM
                     DGGRVLRSALSWRLPRDRATRWAAVVGQVFAVGFVALGLYTNPFLAVIGVFIFLGAGR
                     EERVVRVRAALRGARVRDAMLADPVVLHPSEPLGRCLELAVHRKQEDFAVEADGRLVG
                     ILTRADWAEAIRARGESAEVGSAARRHFMALHPDAPLARVCAELGRMGQPVFPVIESG
                     RLVGLLAVADIERFLALREVRDRPSRSRAAPNRFTIDLG"
     gene            complement(1307..2905)
                     /locus_tag="PK634_12235"
     CDS             complement(1307..2905)
                     /locus_tag="PK634_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOW98546.1"
                     /translation="MKGLKSIAAGLLALVLAASSVAAREIAVTILHTTDVHGFVEPTR
                     DYEGNENVGGLLRCATLIRQARDEAPGALLVDCGDLWIGSPESLVTRTAVARKAVAWL
                     KYDAWVIGNHDFDWGLGLFQKLLESAPAPALGANIRSRPGAPNPAAAVHPYLIREVEG
                     IRVALVGLTTPGMPGWFLPEYLGSLEFEASVPALRRIMPSVREAGAEIRVLLVHQGVR
                     PFGDDEANELERIAAAFPEFDVILGGHSHQVVPGIKIRDVWYSQAGYYATVLGRVDLV
                     YDTVRQGVVRVDCRLLPAGADVEPSAELQAVVQAELDKAAAQRRKIVGRTVRDLVASV
                     EPPGQSPAQQLLAHAIAAETGAEIVLHGILAEDTIPAGPITGGDLWRLVPYENRVGLL
                     SLTAAELKAVLEENVQHLGTPQFQGVCGLAYDLDPAAPEGRRVLNLRLADGSKLHGRK
                     RYRVAMNSYVLASGGRRYPVVRKLVGKSNTRFELTDLDTRMAVARYLRKHRPLDLGPG
                     DEVRLLSRPRPAAAAKASGGAVAP"
     gene            complement(2913..4193)
                     /gene="clpX"
                     /locus_tag="PK634_12240"
     CDS             complement(2913..4193)
                     /gene="clpX"
                     /locus_tag="PK634_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOW98547.1"
                     /translation="MATKPETKRCSFCGRPATEVRQLIAGPQVHICDQCVQLCHSMIE
                     RGPAKEAAKPAKPGLKVPRPHEIKAALDEYVVGQEHAKKVLSVAVHNHYKRLKDVRED
                     WAAPEHADVELEKSNILLVGPTGSGKTLLAKTLARLLDVPFSISDATTLTEAGYVGED
                     VENVLLRLIQAANHDIERASLGIVYIDEIDKIARRMENVSITRDVSGEGVQQGLLKIL
                     EGTVSNVPPQGGRKHPQQEYIKINTEHILFICGGAFVGLEDIVRRRVGKRAIGFGAPA
                     TAAAGPGALLQHVEPEDLVRFGLIPEFVGRLPVVSALKPLTEDDLVRILTEPRNAMVR
                     QYMKLMAMEGVDLTFSNAALRELARRASERGMGARGLRALLERVMLDAMYEVPQKPGA
                     RRLRISRDFVASRLEASESPGKPASAPDKEEQSA"
     gene            complement(4197..4814)
                     /gene="clpP"
                     /locus_tag="PK634_12245"
     CDS             complement(4197..4814)
                     /gene="clpP"
                     /locus_tag="PK634_12245"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOW98548.1"
                     /translation="MTERAQVLIPMVVEQTGRGERAYDIYSRLLKDRIIFIGTAIGDE
                     ISNLVIAQMLFLQSEDAEKDINLYINSPGGSVTAGLAIYDTMQFLKCPVTTYCVGQAA
                     SMAAVLLAAGSAGRRFALPNARIMIHQPWGGVEGQASDISIQAKEILRLRDRLNEILA
                     RHTGKSLEVISRDTDRDFFMAADEAKAYGLVDEVVTGKKGAAKGG"
     gene            complement(4897..6198)
                     /gene="tig"
                     /locus_tag="PK634_12250"
     CDS             complement(4897..6198)
                     /gene="tig"
                     /locus_tag="PK634_12250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOW98549.1"
                     /translation="MNIQIEDIGPCRKSVRVEVPAEQVNATYEEVLGEFAREAKIPGF
                     RPGRAPREVVGKRFAQDIQKEVKDRLVPEGYREAVKQHNLHVIEVLAVDDVSYVAGAP
                     MVFKLTLDVSPDFTLPVYKEIPLQKKKQEVTAAAVDEVVKDILDRAATWEEVAGRPVQ
                     AGDLAMTDYEGVADGKPVAELAPKMEMLGKVTDQLIPAQPESIIPGLGAALVGMSAGE
                     RKQLAVDFPADYFEKALAGRKADYFITVKGVREKKLPAMDAEFFKKMGMESEAALRDQ
                     IRKDLAAAQGQQEHARLRGEIVKYLIEKTTLDLPASVVERETRDAVYDIVSTHRARGV
                     PQETIEEKKEEVFQAAARTAQDRVKLRYILHRIAEQEKVEATEEDLAGRLAELARRYR
                     STPEEVRAELEKKDSLDDIRFEVRLNKVLDFLLAQAKVKEE"
     gene            complement(6300..7523)
                     /locus_tag="PK634_12255"
     CDS             complement(6300..7523)
                     /locus_tag="PK634_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOW98550.1"
                     /translation="MSLEIIQDKCTGCGICLKACPFGALTLEMRDKLGLPPSKFKRIV
                     KVEVSCTLCGACVPTCKFDAMKLDKPEAAAPADRPLDAYRGIWVYVEQRGGHIEGVSF
                     ELLAKGRELADQRKTELVGVLLGHDMKGKVDELFARGADKVILADHPALAGFDSDAYA
                     DVFAELVKKHKPEVVLAGGTSIGRTFIPRVAIRVKTGLTADCTGLEIDPKENLLLQTR
                     PAFGGNIMATIICPNHRPQMATVRHKVFKAGAPDPQRKGTLIEEVMDIRAGRAKVVEV
                     IREMTNEINVAEADIIVSGGRGLGGPDNFKLLFELAKELGAAVGASRGAVDAGWISSF
                     HQVGQTGKTVCPKLYIACGISGAIQHLVGMSSSDTIVAINKDPNAPIFDVADYAIVGD
                     LTEVVPAITKVLRGE"
BASE COUNT         1111 a         2788 c         2588 g         1246 t
ORIGIN      
        1 cccgccgccg tggattcgcg gcgacgacat cctggctctc ggcatcccgc agggcccgcg
       61 cgtcggctac tggcacaaga aggcctacga agcccaactg gagagccggt tcccggaccg
      121 cgaggcgctg ctgaactgga tccgtgagga gatccgggaa cagccgggcg gcggcgttcc
      181 cgcggggcct accccaggtc aatcgtaaag cggttcggcg cagcccggct tcgggacggc
      241 cggtcccgga cctcgcgcag ggcaaggaat cgctcgatgt cggcgacggc cagcaggccg
      301 acgagccggc cgctctcgat cacgggaaag accggctggc ccatccgccc gagctccgcg
      361 cagacccgcg ccagcggggc gtcgggatgc agggccatga aatggcggcg ggcggccgat
      421 ccgacctccg cggattcccc ccgggcgcgg atcgcctcgg cccaatccgc gcgtgtcagg
      481 atccccacca gccggccgtc ggcctcgacg gcgaagtcct cctgtttccg gtggacggcc
      541 aactcgagac agcggcccag cggctcggac ggatggagca cgacgggatc cgcgagcatg
      601 gcgtcgcgga cgcgtgcacc ccggagcgcg gcgcggaccc ggacaacgcg ctcctcgcgg
      661 ccggccccga gaaagatgaa gacgccgatg accgccagga agggattcgt gtagaggccc
      721 agggccacga agccgaccgc gaagacctgc ccgaccaccg ccgcccagcg cgtcgcgcga
      781 tcgcgcggca ggcgccaact caacgcgctg cgcaggacgc ggccgccgtc catcgggaac
      841 gccgggatca ggttgaacac ggccatcacc gcgttggcct gcatcaacgt gtccagccat
      901 tcctgccagg cgccggccgg cggctccgcc gcgatccagg acggaaaccc gccgcggatc
      961 agcgccagcc cgaggaagag ggccacgttc accgccgggc ccgcgaccgc cacgagaaac
     1021 tcgtggagcg ggcgctcggg aattttcctc atgagcgcca cgcctccgat cggcagcagc
     1081 gtgatcgcct gcacctggcc gccgaaccgg atcgcgacca ggctgtgccc cagttcgtgg
     1141 aggaccacgc aggcgaagag cccggccagc agggacaggg cccaggccgc cgcccgccat
     1201 ccatccccct cgccgcccca ccaggcgaac cagccggcca gcaggaagaa ggtccagtgg
     1261 atccggatgt ccacgccgaa aaggcgcccg atggagaacg accccttcac ggggccaccg
     1321 cgccgccgga cgctttggcg gccgcggccg ggcgggggcg ggagagcagg cgcacttcgt
     1381 cgccgggccc gaggtccagc gggcggtgtt tccggaggta gcgggccacg gccatgcggg
     1441 tatccaggtc ggtcaactcg aaacgggtat tgctcttccc gaccagcttc cgcacgaccg
     1501 ggtagcggcg gccgccggac gccaggacgt agctgttcat ggcgactcgg taccgcttgc
     1561 ggccgtgcag cttggagccg tccgccagcc gcaggttgag cacccggcgg ccctccggcg
     1621 ccgcgggatc gaggtcgtag gccagcccgc agacgccctg gaactgcggg gtgcccaggt
     1681 gctgaacgtt ttcttccagc acggctttca gttccgcggc ggtcagggag agcaggccca
     1741 cccggttctc gtaggggacc agccgccaca ggtccccgcc ggtgatcggc cccgcgggga
     1801 tcgtgtcttc cgccaggatg ccgtgcagca caatctccgc gcccgtctcc gccgcgatcg
     1861 cgtgcgcgag gagctgctgc gcgggggact ggccgggcgg ctcgacggag gcgacgaggt
     1921 cgcgaacggt gcgccccacg atcttgcggc gctgcgcggc ggccttgtcg agttcggctt
     1981 gcacgaccgc ctgcaattcc gccgacggtt ccacgtcggc cccggcgggc agcagccggc
     2041 agtccacgcg caccacgccc tgccggacgg tgtcatagac caggtccacc cggccgagca
     2101 cggtggcgta gtagccggcc tgcgagtacc agacgtcgcg gatcttgatg cccggcacga
     2161 cctggtgcga atggccgccg aggatgacgt cgaattccgg gaacgcggcc gcgatccgtt
     2221 ccagctcgtt ggcctcgtcg tcgccgaagg gccggacgcc ctggtgcacc agcagcacgc
     2281 ggatctccgc gccggcctcc cgaacggacg gcatgatccg ccgcagggcc gggacagacg
     2341 cttcgaactc cagggagccg agatactcgg gcaggaacca gccgggcatg cccggcgtgg
     2401 tcagccccac gagggccacg cggatgcctt ccacctcgcg gatcaggtac ggatggacgg
     2461 ccgccgccgg gttcggcgcg ccgggccggg accggatgtt cgcgcccagc gcgggggccg
     2521 gggccgactc gagcaacttc tggaacaggc ccagccccca atcgaaatcg tggttcccga
     2581 tcacccaggc gtcgtatttc agccaggcca cggccttgcg ggcgacggcc gtcctggtga
     2641 ccagactttc cgggctgccg atccagaggt cgccgcagtc cacgagcagc gcgccgggcg
     2701 cctcgtcccg cgcctgccgg atcagggtcg cgcaccgcag cagcccgccg acgttctcgt
     2761 tcccctcgta atcgcgggtg ggctcgacga agccgtgcac gtcggtcgtg tgcaggatcg
     2821 tcacggcgat ttcgcgggcg gcgacggacg acgccgccag gaccagcgcg aggagtccgg
     2881 ccgcgatgga tttcaacccc ttcacgcccg cctcacgcgc tctgctcttc cttgtccggc
     2941 gcggaggccg gcttcccggg cgattcggac gcctccagcc tggaggccac gaagtcccgg
     3001 ctgatccgga gccgccgggc gcccggcttc tgcgggacct cgtacatggc gtcgagcatc
     3061 acgcgctcaa gcagggcgcg caggccgcgg gcgcccatcc cgcgctcgct cgcgcggcgg
     3121 gccagctcgc gcagggcggc gttgctgaag gtcaggtcca cgccctccat agccatgagt
     3181 ttcatgtatt gtcggaccat ggcgttgcgc ggctcggtaa ggatccggac gagatcgtcc
     3241 tccgtcagcg gcttgagcgc ggagaccacc gggaggcggc cgacgaactc ggggatcagc
     3301 ccgaagcgga ccaggtcctc cggctcaacg tgctgcagca gcgcccccgg gccggcggcc
     3361 gccgtcgcgg gcgccccgaa gccgatcgcc cgcttgccca cgcggcgccg gacgatgtcc
     3421 tccagcccga cgaacgcgcc gccgcagatg aagaggatgt gctccgtgtt gatcttgatg
     3481 tattcctgct gcgggtgctt ccggccgccc tgcggcggca cgttggagac ggtcccctcc
     3541 aggatcttca gcaggccctg ctgcacgcct tcgcccgaca cgtcgcgggt gatggacacg
     3601 ttctccatcc ggcgcgcgat cttgtcgatc tcgtcgatgt acacgatgcc caggctggcg
     3661 cgctcgatgt cgtggttcgc ggcctggatg agccggagca ggacgttctc gacgtcctcg
     3721 cctacgtagc cggcctcggt cagcgtcgtc gcgtcggaaa tgctgaacgg cacgtccagc
     3781 aggcgggcca gcgtcttggc cagcagcgtc ttgccggagc ccgtcgggcc gacgagcagg
     3841 atgttgctct tctcgagctc gacgtccgcg tgctcgggcg ccgcccagtc ctcgcgcacg
     3901 tccttcagcc gcttgtagtg gttgtggacg gcgacggaga gcaccttctt ggcgtgctcc
     3961 tggccgacga cgtactcgtc cagcgcggcc ttgatctcgt gggggcgcgg caccttgagg
     4021 ccgggcttcg cgggcttggc cgcctccttc gcgggccccc gctcgatcat cgagtggcag
     4081 agctggacgc actggtcgca gatatggacc tgcgggccgg cgatcaactg ccgcacctcg
     4141 gtagccgggc ggccgcagaa cgaacagcgc ttcgtttccg gcttcgtggc catggctcac
     4201 ccgcctttcg cggcgccctt cttccccgtg acgacctcgt ccaccaaccc gtaggctttc
     4261 gcctcgtcgg cggccatgaa gaaatcgcgg tccgtgtccc gcgagatcac ctcgagcgac
     4321 ttgcccgtgt gccgcgcgag gatctcgttg aggcggtcgc gcagccgcag gatctccttg
     4381 gcctggatgc tgatgtccga ggcctgcccc tccaccccgc cccacggctg gtggatcatg
     4441 atccgcgcgt tcggcagggc gaaccgccgt cctgccgagc ccgcggccag caggaccgcc
     4501 gccatgctcg ccgcctgccc gacgcagtag gtcgtcacgg ggcacttgag gaactgcatc
     4561 gtgtcgtaga tcgccagccc ggccgtcacc gagccgcccg gcgagttgat gtagaggttg
     4621 atgtccttct cggcgtcctc gctctggagg aacagcatct gcgcgatcac caggttgctg
     4681 atctcgtcgc cgatcgccgt gccgatgaag atgatccggt ccttcaggag gcgcgagtag
     4741 atgtcgtacg cgcgctcgcc gcgccccgtc tgctccacca ccatcggtat gagaacctgc
     4801 gcccgctcgg tcatagtgcc tccccgctct gcgccccgtg ccccggcagg gcgggcccgg
     4861 cccgggcctg ggcgccctgc cgcccaacca tggatactat tcctctttta ccttcgcctg
     4921 cgcgagcagg aaatccagca ccttgttcag ccggacctcg aaccggatgt cgtccaggct
     4981 gtccttcttc tccagctcgg cccggacctc ttccggggtc gagcggtagc gccgcgccag
     5041 ttccgccagc cggccggcga ggtcctcctc ggtcgcctcg accttctcct gctcggcgat
     5101 ccggtgcagg atgtagcgca gcttgacccg gtcctgggcc gtgcgggccg cggcctggaa
     5161 gacctcttcc ttcttctcct cgatcgtctc ctgcggcacg ccgcgggcgc ggtgggtgct
     5221 gacgatgtcg tacaccgcgt cccgcgtctc gcgctcgacg acggacgccg gcaggtcgag
     5281 ggtcgtcttc tcgatcaggt acttgacgat ctccccgcgc agccgggcat gctcctgctg
     5341 cccctgcgcc gcggccaggt ccttgcgaat ctggtcccgg agcgccgcct cggattccat
     5401 gcccatcttc ttgaagaact ccgcgtccat cgcgggcagc ttcttctcgc gcacgccctt
     5461 gacggtgatg aaatagtcgg ccttcctgcc cgccagcgcc ttctcgaagt agtcggcggg
     5521 gaagtccacg gccagctgct tgcgctcccc ggcggacatc ccgaccagcg ccgccccgag
     5581 gcccgggatg atcgattccg gctgggccgg gatcagctgg tccgtgacct tgcccagcat
     5641 ctccatcttc ggcgccagct cggcaaccgg cttgccgtcg gccacgccct cgtagtccgt
     5701 catggccagg tcgcccgcct gcaccgggcg gccggccacc tcctcccacg tcgccgcgcg
     5761 gtcgaggatg tccttcacga cctcgtccac ggcggcggcg gtgacctcct gcttcttctt
     5821 ctggagcggg atttccttgt agaccggcag cgtgaagtcc ggcgacacgt cgagcgtcag
     5881 cttgaacacc atcggcgcgc ccgcgacgta cgagacgtcg tccacggcca ggacctcgat
     5941 cacgtgaagg ttgtgctgct tcacggcctc gcggtagccc tcgggaacga ggcggtcctt
     6001 gacttccttc tggatgtcct gcgcgaaccg cttgccgacg acctcgcgcg gcgcccggcc
     6061 cggccggaag cccgggatct tcgcctcgcg ggcaaactcg cccaggacct cttcatacgt
     6121 agcgttgacc tgctccgcgg gcacttccac gcggacggac ttgcggcagg gaccgatgtc
     6181 ttcgatttgg atgttcattg tcagggatgg ggttcggggt tcccgccttc gccagggcga
     6241 cggcggacca gcagggttca gggttcagtt ttcaggaaaa tccattcacc attcactatt
     6301 cactcccccc tgagcacctt cgtgatcgcc ggcaccacct cggtcaggtc gccgacgatg
     6361 gcatagtcgg ccacgtcgaa gatcggcgcg ttggggtcct tgttgatggc gacgatggta
     6421 tccgaggagc tcatgccgac gaggtgctgg atcgcgccgg agatgccgca ggcgatatag
     6481 agcttcgggc acacggtctt gcccgtctgg ccgacctggt ggaaggacga gatccagccc
     6541 gcgtccaccg cgccgcggct cgcgccgacg gccgcgccca gctccttggc cagttcgaag
     6601 agcagcttga agttgtccgg cccgcccagc ccgcgcccgc cggacacgat gatgtccgcc
     6661 tcggccacgt tgatctcgtt ggtcatctcg cggatgacct cgacgacctt cgcccggccg
     6721 gcccggatgt ccatcacctc ttcgatcagc gtccccttcc gctgcgggtc gggggcaccg
     6781 gccttgaaga ccttgtggcg caccgtagcc atctgcggcc ggtggttcgg gcagatgatg
     6841 gtggccatga tgttgccgcc gaacgcgggc cgggtctgga gcaggaggtt ttccttgggg
     6901 tcgatctcga ggcccgtgca gtccgccgtc aggccggtct tcacccggat cgcgacgcgc
     6961 gggatgaacg tgcggccgat gctcgtgccg ccagcgagga cgacctcggg cttgtgcttc
     7021 ttgaccagtt cggcaaagac gtccgcgtag gcgtccgaat cgaaaccggc cagcgccgga
     7081 tggtccgcca ggatcacctt gtccgcgccg cgcgcgaaca attcgtccac cttgcccttc
     7141 atgtcgtggc ccagcaggac gcccaccagc tcggtcttcc gctggtcggc cagctcgcgg
     7201 cccttggcca gcagctcgaa cgagacgccc tcgatatgcc cgccgcgctg ctcgacgtag
     7261 acccagatgc cccggtacgc gtcgagcggc cggtccgccg gcgcggcggc ctcgggcttg
     7321 tccagcttca tggcgtcgaa cttgcacgtc gggacgcagg cgccgcacag cgtgcaggag
     7381 acctcgacct tgacgatgcg cttgaacttg ctcggcggca ggccgagctt gtcgcgcatc
     7441 tccagcgtca gcgcgccgaa cgggcaggcc ttgaggcaga tcccgcagcc cgtgcatttg
     7501 tcctggatga tttcaagcga catggcgaac tcactccatc ccggggccgc ggccccgtta
     7561 aatcaccttc tgctccttca ggcggctgac gatctcgcgg gcggcgacgc tcggctcggt
     7621 gccgtcgatg cgcacgccgg agcttttctt gggcggggtg aagatcttca ccacgcgggt
     7681 cggcgagccc ttcaggccga tctccttcgg gtccgccttc acggcctcgg cgg
//
LOCUS       DAOOTK010000134         7680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_5946_length_7680_cov_5.828721, whole genome shotgun sequence.
ACCESSION   DAOOTK010000134 DAOOTK010000000
VERSION     DAOOTK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5946_length_7680_cov_5.828721"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /gene="bamA"
                     /locus_tag="PK634_12260"
     CDS             <1..1297
                     /gene="bamA"
                     /locus_tag="PK634_12260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOW98551.1"
                     /translation="DPATHVADVRYTVTEGREAHIREIRIRGNSRTKDKVIRRELAVY
                     PGELYNTVKVRTSERRLQNLNYFEYVNSYPSPTDDPGWYDMNIEVEEQRTGQLMAGAG
                     FSSVDDLIGFVELSQGNFDLFGWPGMTGGGQKLKLRAQVGTKRSDYELSFIEPWFLNR
                     RLSLGLDLFRHENSYDSSLYDQRNVGGRVTLGHALGAFSRVNLSYGLQQIDIYDVSTN
                     ASSAIRDEEGTYKKSEVTLELVRDTRDHPFLPTRGNRSSVSGSVAGGPLGADTDVYGF
                     EARSSQYFPLWFDHVFNVRGWAGVVEEYGDSDEVHLFDRLFLGGPRTVRGFKYRDVGP
                     HDENGEPIGGNTAGYLTAEYTLPVAEKVRLAFFYDVGMVWPESWDAELNDLNSGLGFG
                     VRFDLPGFPIQLDYTWPIDTDPENDRDSGRFSFWLGYQY"
     gene            1323..1952
                     /locus_tag="PK634_12265"
     CDS             1323..1952
                     /locus_tag="PK634_12265"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOW98552.1"
                     /translation="MKSSAVKRAAAITLAVGFGLALAARAETPPLVFVDMERAFTEFY
                     KTKLADEQLKKQAEEFNEERKKLTEEFEKLQEQFNLLRDEAQNTALSEDARAQKRNAA
                     EEKLIETRDMETRIRRFDEARQKQLDDQTRRMRKRIVDEIQQTIQTYARTQGLSTVLD
                     SSGQSFNGVELVLYQDVRSDITDIILEQLNKTEGQAEPAAAPAAEETEK"
     gene            1975..3015
                     /gene="lpxD"
                     /locus_tag="PK634_12270"
     CDS             1975..3015
                     /gene="lpxD"
                     /locus_tag="PK634_12270"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOW98553.1"
                     /translation="MSWTAAELAQRVGATVEGDGTVAITGVAGLREARPGQLSFLANM
                     RYAPDAATTQASAVVVPKDWNRPCAAPCRLLARDPDKAFARIAIEYAPAPVSPPPGVH
                     PTAVIAPDAKLGANVSIGPYVVIEPGASVGEGTVLFAGCYLGHGVRVGAACRFYPQVT
                     VREGCRIGDRAILHNGVVIGSDGFGYTVNEKGVREKIPQIGIVELGHDVEIGANTTID
                     RARFGRTRIGNGVKIDNLVQIGHNVVIGDNAVIVAMVGISGSSEIGERTILAGQVGVV
                     GHIVIGRDCIVGARSVVTKDIAPQSFVSGYPAIPHDEEKRLHAHIARLPEFKKKVAEL
                     EARLKALEARGA"
     gene            3063..4571
                     /locus_tag="PK634_12275"
     CDS             3063..4571
                     /locus_tag="PK634_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOW98554.1"
                     /translation="MLNPEQEENPPSLRRREVSRATRGVVAVTAGQVVSIAISLALAL
                     VIPRLLGARDFGNWALFRSVIDFIALLCALGTPAVMSQYYVGARAAGREEEAGLIFKS
                     VAATRFIVAVLAATAGGLLLLASRNPLMTGAAAGFLALSVLARVAGLAPFLLLYAERR
                     AGRIVCINVAQALLVPVAVAGAYVAGGFRWVPPACAVGDLALAGLNWVVAWPDVRWPR
                     GWLTRARFRELASFGIMLALAHTSAMAYITMAPYFMSLAGMSSVLIGRVGLAVRLQNV
                     LLGLLGTAGGALWPSITILMETEGVRRSVVWQSLFSRLGGVLILAAAGVFMTVAGWAV
                     PFVLGKDFTPAIPVISLWLLMTLFFWLGMRYALFGQLMKSPGRVLKASLWMFAVFIPL
                     FLLFGRTGSGVETVWALLAGTLVYALYLAITTRRKWGISLDSIRLLLPLAVTAGLAVY
                     AIRAGPGGLGWPAPAAWLVVFVASVFVSRSLRVHELTGIISSLRRPGLGGGA"
     gene            4568..5677
                     /locus_tag="PK634_12280"
     CDS             4568..5677
                     /locus_tag="PK634_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOW98555.1"
                     /translation="MSGPLRLAILSLHRGGMAQCAAGLANAVLQARPEAAVAFFGPQD
                     MDPDLFEPGVALFRYRTPQELSWRAVGRLARLPWLARRLRQDILAWRPTVLHVNSGHL
                     SYPLFVPGLAARIPMVSSIHDIFPHLGERRPVERFKLSSLLGHSRRFLVHGRRLKEQA
                     VARWRIDPERIEVFPIIKPSRIRSWAAAAEEQPFDILLFGRIHAYKGVGLILEALPEI
                     VKRVPQARLIIAGKGDLDAWSASLESVRGHVELHNRFIPDREMAAFFRRCALAVLPYL
                     EASQSGIEPVAACFGKPVVATRVGSLDEVVEDGRTGRLVPPGDAGALALAVATLLADP
                     ALRREMGRRAFERRYGPGVEKELGERLCRLYAAAV"
     gene            5692..>7680
                     /gene="uvrB"
                     /locus_tag="PK634_12285"
     CDS             5692..>7680
                     /gene="uvrB"
                     /locus_tag="PK634_12285"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOW98556.1"
                     /translation="MDVFRLKSGFAPTGDQPEAIEALCRGLADGRRFQTLEGVTGSGK
                     TFTIANVIQRWGRPTLVISHNKTLAAQLYAELKGFFPDNAVEYFVSYYDYYQPEAYIP
                     QTDTYIEKDSAINDEIERLRLAATDALINRRDVIIVASVSCIYGLGSPEDYRDMLVNL
                     RRGEPADRDAVLRKLVDIQYSRNDISTEPGTFRARGDAVDIFPSYRKDGVRIDFFGDS
                     VERIRRIDPLTGHTLAELPGVMISPARHFVMPQAKIERALAGIRAELEDQVKKFEAAE
                     KLLEAQRLRMRTEYDIEMLLEVGYCSGIENYSRHLSGRAPGERPATLLDFFQGEFLTI
                     LDESHVSVPQLRGMYNGDRSRKLVLVEHGFRLPSALDNRPMNFDEFLAVTGPILFTTA
                     TPGPFEREVGWPPVPQVIRPTGLLDPPVEVRPLAGQIDDLIEEVRKRAEKGERTLVTT
                     LTKKTAEDLSEYLHNIGLRVKYLHSEIDTIERVEILRGLRKADFDCLVGINLLREGLD
                     LPEVSLVAVLDADKEGFLRSETSLVQTAGRAARHLNGEVILYADQVTESMRRMIDITR
                     ARREKQLAYNREHGITPKSIVKAIQDSLAEQEAAKEVESQAIREAGVEYDAAQVMADI
                     EREMMEAAEALEFERAALLRDQLYELKAAQKKQEKGYST"
BASE COUNT         1211 a         2604 c         2655 g         1210 t
ORIGIN      
        1 cgacccggcg acccacgtgg ccgacgtgcg ctacacggtc acggagggcc gcgaggccca
       61 tatccgggaa atccggatcc gcggcaattc gcggacgaag gacaaggtga tccgccgcga
      121 gctggccgtg tacccgggcg agctctacaa caccgtcaag gtgcggacca gcgagcgccg
      181 gctgcagaac ctgaactact tcgagtacgt caacagctac ccgtcgccca cggacgaccc
      241 gggctggtac gacatgaaca tcgaggtcga ggagcagcgc acgggccagc tcatggccgg
      301 cgcggggttc tccagcgtgg acgacctgat cggcttcgtc gagctgtcgc agggcaactt
      361 cgacctcttc gggtggcccg ggatgacggg cggcggccag aagctgaagc tgcgcgccca
      421 ggtgggcacc aagcggtcgg actacgagct gtcgttcatc gagccgtggt tcctgaaccg
      481 gcggctgtcg ctcggcctgg accttttccg gcatgagaac agctacgaca gcagcctgta
      541 cgaccagcgg aacgtgggcg ggcgcgtgac cctcgggcac gccctgggcg cgttcagccg
      601 cgtcaacctg tcgtacggcc tgcagcagat cgacatctac gacgtgtcga ccaacgcctc
      661 gagcgcgatc cgggacgagg aggggacgta caagaagagc gaggtgaccc tcgaactcgt
      721 gcgcgacacc cgcgaccacc cgttccttcc cacccgcggc aaccgcagca gcgtttccgg
      781 ctccgtcgcc ggcggcccgc tcggggcgga cacggatgtg tacggcttcg aggcccgcag
      841 ctcgcagtat ttccccctct ggttcgacca cgtgttcaac gtccgcggct gggcgggggt
      901 ggtggaggag tacggcgact ccgacgaggt ccacctgttc gaccggctct tcctcggcgg
      961 cccgcgcacg gtccgcggct tcaagtaccg cgatgtcggc ccgcacgacg agaacggcga
     1021 gcccatcggc ggcaacacgg ccggctacct gaccgccgag tacacgctgc ccgtggcgga
     1081 gaaggtccgc ctggccttct tctacgacgt cggcatggtg tggcccgaat cctgggacgc
     1141 ggaactgaac gacctgaatt ccggcctggg cttcggcgtc cggttcgacc tgcccgggtt
     1201 cccgatccag ctggactaca cgtggccgat cgatacggat cccgagaacg accgggatag
     1261 cgggcgcttc agcttctggc tgggctacca gtactgatcc tttcacaacg cacggaggaa
     1321 acatgaagag cagcgcggtg aagagagcgg cggcgatcac gttggcggtg ggcttcggcc
     1381 tggccctggc ggcccgggcc gagacgcccc cgcttgtctt tgtggacatg gagcgggcgt
     1441 tcaccgagtt ctacaagacc aagctcgccg acgagcagct gaagaagcag gcggaggaat
     1501 tcaacgagga gcgcaagaag ctcacggagg agttcgagaa gcttcaggag cagttcaacc
     1561 tcctgcgcga cgaggcgcag aacacggcct tgagcgagga cgcgcgcgcg cagaaacgca
     1621 acgcggccga ggagaagctg atcgagaccc gcgacatgga aacgcgcatc cggcgcttcg
     1681 acgaggcccg gcagaagcag ctcgacgacc agacccggcg catgcgcaag cggatcgtcg
     1741 acgagatcca gcagacgatc cagacctatg cccgcacgca gggcttgagc accgtgctcg
     1801 attcctccgg gcagagcttc aacggcgtcg aactggtgct ctaccaggat gtccgcagcg
     1861 acatcacgga catcatcctg gagcagctca acaagaccga gggccaggcc gaaccggcgg
     1921 cggcgcccgc ggcggaagag acggagaagt gagattcttg gcgcggagga acacatgagc
     1981 tggacggcgg cggaactggc gcaacgcgtg ggcgccacgg tcgagggaga cgggacggtc
     2041 gcgatcacgg gcgtggcggg cctccgcgag gcgcggcccg gccagctgtc ctttcttgcg
     2101 aacatgcgct acgccccgga cgcggccacc acgcaggcgt ccgccgtcgt ggttccgaag
     2161 gactggaacc ggccctgcgc ggcgccctgc cggttgctcg cccgggatcc ggacaaggcc
     2221 ttcgcccgga tcgccatcga gtacgccccc gcgccggtct cgccgccgcc gggcgtgcac
     2281 ccgacggccg tcatcgcccc cgacgcgaag ctcggcgcca acgtcagcat cgggccctac
     2341 gtcgtcatcg agcccggcgc gtccgtgggc gaggggaccg tcctcttcgc cggctgctac
     2401 ctcggccacg gggtcagggt cggcgcggcg tgccggttct acccgcaggt cacggtccgc
     2461 gaaggctgcc gcatcggcga ccgcgcgatc ctccataacg gcgtggtgat cgggagcgac
     2521 ggattcggat acaccgtcaa cgagaagggc gtccgggaaa agatcccgca gatcggcatc
     2581 gtcgagctcg gccacgacgt ggagatcggc gccaacacga cgatcgaccg cgcccggttc
     2641 gggcgcacgc ggatcggcaa tggcgtgaag atcgacaacc tggtccagat cggccacaac
     2701 gtggtcatcg gcgacaacgc cgtgatcgtc gcgatggtcg gcatctccgg cagctcggaa
     2761 atcggagagc gcaccatcct ggcgggccag gtcggcgtgg tggggcatat cgtgatcggg
     2821 cgcgactgca tcgtcggggc ccggtcggtg gtcaccaagg acatcgcacc gcaatcgttc
     2881 gtgtccgggt atccggcgat ccctcatgac gaggaaaagc gattgcacgc ccatattgcc
     2941 cgcctgccgg agttcaagaa aaaggtggcg gagttggaag cgcggctgaa ggccctggaa
     3001 gcccggggcg cctgaccgct ccgcgccttt cctgtcgctc catgccgggc gagccggtcg
     3061 tcatgctgaa tccggaacag gaagagaacc cgccgtcatt acgccgcagg gaggtgtccc
     3121 gggccacgcg gggggtcgtg gccgttacgg ccgggcaggt cgtgagcatc gcgatttcgc
     3181 tggcccttgc cctggtcatc ccgcggctgc tgggcgcgcg cgatttcggc aactgggcgc
     3241 tcttccgaag cgtgatcgat ttcatcgcgc tgctatgcgc gctcggcacc ccggccgtca
     3301 tgtcccagta ctacgtgggc gcgcgcgccg cgggccggga ggaagaggcc ggcctgattt
     3361 tcaaatcggt ggcggcgacc cgcttcatcg tggcggttct cgcggcgacg gccgggggcc
     3421 tgctgctgct cgcgagccgg aatcccctga tgacaggcgc ggccgccggc ttcctggcct
     3481 tgagcgtcct cgcccgcgtc gcggggctgg cgcctttcct cctcctgtat gccgagcgcc
     3541 gggcgggccg gatcgtctgc atcaacgtcg cgcaggcgct cctcgtgccc gtggccgtgg
     3601 ccggcgcgta tgtcgccgga gggttccgct gggtgccgcc cgcgtgcgcc gtgggcgacc
     3661 tcgcgctcgc cggcctcaac tgggtcgtcg cctggccgga tgtccggtgg ccgcggggct
     3721 ggctgacgcg ggcccgcttc cgggaattgg cctcgttcgg aatcatgctc gcgttggccc
     3781 acacgagcgc gatggcctac atcaccatgg ccccgtactt catgagcctc gccgggatga
     3841 gcagcgtgct gatcgggcga gtgggcctcg ccgtgcggct ccagaatgtc ctgctcggcc
     3901 tgctcggcac ggccggcggg gcgctctggc cctccatcac gatcctcatg gaaaccgagg
     3961 gcgtccggcg cagcgtggtc tggcagagcc tgttcagccg cctcgggggc gtgctgatcc
     4021 tcgccgctgc cggcgtcttc atgacggtgg ccggctgggc cgtgcccttc gtcctgggga
     4081 aggatttcac gccggccatc cccgtcatca gcctgtggct gctcatgacc ctgttcttct
     4141 ggctggggat gcggtacgcc ctgttcgggc agttgatgaa aagccccggc cgggtgctga
     4201 aggcctccct gtggatgttc gcggtgttca tcccgctgtt cctgctcttc ggcaggacgg
     4261 ggtccggcgt cgagacggtc tgggccctgc tcgcgggcac gctcgtctac gcgctgtacc
     4321 tggcgatcac gacccggcgg aaatggggga tcagcctgga ctcgatccgg ctgctgctcc
     4381 cgctggccgt gacggccggc ttggcggtct acgccattcg ggccggtccg ggcggcctcg
     4441 gctggccggc tcccgcggcc tggctggtcg tcttcgtggc cagcgtgttc gtttcccgca
     4501 gcctgcgcgt ccacgaattg accggcatta tctcctcctt gaggcggccg ggcttgggag
     4561 gaggggcatg agcgggcccc tccggctggc gatcttgagc ctgcaccggg gcggtatggc
     4621 gcagtgcgcg gccggtctgg ccaacgccgt gctccaggcc cggccggagg ccgccgtggc
     4681 cttcttcggc ccgcaggaca tggacccgga cctgttcgag cccggcgtgg cgttattccg
     4741 gtaccgcact ccgcaggaac tgtcctggcg cgcggtcggc cgcctcgccc gcctgccgtg
     4801 gctcgcccga cggctgcggc aggacatcct cgcatggcgg ccgaccgtgc tccacgtgaa
     4861 ctccggccac ctttcctatc ccctgtttgt gccgggcctc gccgcccgga ttcccatggt
     4921 atccagcatc cacgatatct tcccgcatct cggggagagg cggccggtgg agcggttcaa
     4981 gctgtcctcc ctgctggggc acagccgtcg tttcctggtc cacgggcggc ggctcaagga
     5041 gcaggccgtc gcccgctggc gtatcgaccc ggaacgcatc gaggtgtttc cgatcatcaa
     5101 gccctcgcgg attcgttcct gggcggcggc cgcggaggag cagcccttcg acatcctgct
     5161 gttcggccgg attcacgcgt acaagggcgt gggcctgatc ctggaggccc tgccggagat
     5221 cgtgaagcgc gttccgcaag cgcggctgat catcgccggg aaaggggacc tcgacgcctg
     5281 gtcggcttcg ctcgagtccg tgcgcgggca cgtggaattg cacaatcggt tcattcctga
     5341 ccgcgagatg gcggctttct tccggcgctg cgccctggcc gtgctgccgt acctggaggc
     5401 cagccagagc gggatcgagc cggtcgccgc ctgctttggc aagcccgtcg tggccacccg
     5461 ggtcggctcc ttggacgagg tcgtcgagga tggacggacg gggcggctgg tgccgcccgg
     5521 ggatgccggg gccctggccc ttgccgtcgc gaccctgctg gccgatcccg ccctgcgccg
     5581 ggagatgggc cggcgggcct ttgagcggcg gtacgggccg ggggtggaaa aggaacttgg
     5641 cgagcggctc tgccgtttgt acgcggcagc ggtttaggtt aggatgccgg catggacgtt
     5701 ttccggctta aatccggctt cgcgccgacc ggcgaccagc ccgaggcgat cgaggccctg
     5761 tgccgcgggc tggcggacgg gcgccggttc cagaccctcg agggcgtcac gggctccggc
     5821 aagacgttca cgatcgccaa cgtgatccag cgctggggcc ggccgaccct cgtcatctcg
     5881 cacaacaaga cgctggccgc ccagctctac gccgagctga agggcttctt cccggacaac
     5941 gccgtcgagt acttcgtcag ctactacgac tactaccagc ccgaagccta catcccgcag
     6001 accgacacct acatcgagaa ggactccgcg atcaacgacg agatcgagcg cctgcgtctc
     6061 gcggcgaccg acgccctgat caaccgccgc gacgtcatca tcgtggcctc cgtctcgtgc
     6121 atctacggcc tgggctcgcc cgaggattac cgcgacatgc tggtcaacct gcgccgcggg
     6181 gagcccgcgg accgcgacgc cgtgctgcgc aagctggtcg acatccagta ctcgcgcaac
     6241 gatatctcca ccgagcccgg cacgttccgc gcgcgcggcg acgcggtgga catctttccg
     6301 tcctaccgca aggacggcgt gcggatcgat ttcttcggcg actcggtgga acgcatccgg
     6361 cgcatcgatc cgctgaccgg ccacacgctt gccgagctgc cgggcgtcat gatctcaccc
     6421 gcgcgccact tcgtcatgcc ccaggccaag atcgagcgcg ccctggccgg catccgcgcc
     6481 gagctggagg accaggtgaa gaagttcgag gccgcggaga agctgctgga ggcccagcgc
     6541 ctgcgcatgc gcacggaata cgacatcgag atgctcctcg aagtcggcta ctgcagcggc
     6601 atcgagaact attcccgcca cctctccgga cgcgcccccg gcgagcggcc cgcgacgctc
     6661 ctggacttct tccaggggga attcctgacc atcctcgacg agtcgcacgt cagcgttccg
     6721 cagcttcgcg ggatgtacaa cggcgaccgg tcgcgcaagc tggtcctggt ggaacacggg
     6781 ttccggctgc cgtcggccct cgacaatagg ccgatgaatt tcgacgagtt tctggcggtc
     6841 acgggcccga tcctgttcac cacggccacg cccggcccgt tcgagcggga ggtcggctgg
     6901 ccgcccgtcc cgcaggtcat ccgcccgacc ggcctgctgg atccgcccgt cgaggtgcgc
     6961 ccgctggccg gccagatcga cgacctgatc gaggaggtcc ggaagcgggc cgagaagggc
     7021 gagcggacgc tcgtcaccac gctgaccaag aagaccgccg aggacctgtc cgagtacctc
     7081 cacaacatcg ggctgcgcgt gaagtacctg cactcggaaa tcgacaccat cgagcgggtc
     7141 gagatcctgc gcggcctgcg caaggcggac ttcgactgcc tcgtgggcat taacctcctg
     7201 cgcgagggac tagacctgcc cgaggtttcg ctcgtcgccg tcctggacgc ggacaaggag
     7261 gggttcctgc gctcggagac ctcgctcgtg cagacggcgg gccgcgccgc gcggcacctg
     7321 aacggggagg tcatcctgta cgccgaccag gtgacggaat ccatgcggcg catgatcgac
     7381 atcacgcgcg cgcgccgcga gaagcagctc gcgtacaacc gcgagcacgg catcacgccg
     7441 aagagcatcg tcaaggccat ccaggacagc ctcgcggagc aggaggccgc gaaggaggtg
     7501 gagtcgcagg ccatccgtga ggccggcgtg gagtacgacg cggcgcaggt gatggcggac
     7561 atcgagcgcg agatgatgga ggccgccgag gcgctggaat tcgagcgcgc cgcgctgctg
     7621 cgcgaccagc tctacgagct caaggccgcg cagaagaagc aggaaaaagg ctattcgacg
//
LOCUS       DAOOTK010000135         7591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6039_length_7591_cov_5.166269, whole genome shotgun sequence.
ACCESSION   DAOOTK010000135 DAOOTK010000000
VERSION     DAOOTK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6039_length_7591_cov_5.166269"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="PK634_12290"
     CDS             <1..1924
                     /locus_tag="PK634_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOW98557.1"
                     /translation="NIVSKFETMDNHWEAARLGASEMSVAVLASAGTNVIVMLPIAMM
                     TSLAGRMLTPFAIATLIVNAVSILISFTLTPILAALLLEPASRRRDNAFARFGKRWEA
                     GFQTVGVGFSNALRRIAGKKRYNLPVVAGFILFFFFTMKFGGSRLGFSFFETPDMARV
                     FIRVEMPPYYDLEKTVARIDEVQSRLLAFSDLEHVLTTAGKAESMSGQASEGVYMGQI
                     ELFFKPKTERPWNLLDRLDEIRASLKDETDCFVSASVPGHMGGQSFQIDQNLCGEDLD
                     VLDNTAIAIRDAAAGLPGVAMIETTVRDTKPEIRVLPKRPVLSDLGLPAHLLASIVRA
                     NVEGLEAADFKRGDRTYDIRVKLAEQPGKDQIRQFLLPGADGRPIPLETVADVVDARS
                     KVQIYRYDKRRTVKILGDLKPGATMSGISDAMARTIADRKLLPAGYTLGNTGMIEMME
                     DTIADFGEAILLATFLTLLTLAAILESWKRPALILLTLPMGLIGIVWSLALTGKAITM
                     LVMLGSLMLIGIVVNAAILIVDKMAQHIKDGISRRDAMLTAMVEEFRPVLMVILASGL
                     GMLPMAIGAGIGSENRAGIGIASVAGIMVAGFFTMTILPLIYTLFTGKPRASHDAGSR
                     GERNPPEPAGPPPQAG"
     gene            1974..2363
                     /locus_tag="PK634_12295"
     CDS             1974..2363
                     /locus_tag="PK634_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW98558.1"
                     /translation="MPLKTTVEKRRQGGYLVRLQGRLDSETTPGCEKKILPLLEEKVH
                     TVVLEAAGLEYISSAGLRLVLELRKQMAARKGHLALAHLQPAVAQVFEVADILPRTGI
                     FDSVESADMFLDAIQRREQVKSVDETD"
     gene            2497..5484
                     /locus_tag="PK634_12300"
     CDS             2497..5484
                     /locus_tag="PK634_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOW98559.1"
                     /translation="MAIRLEVSLNPSIPDARGRDTAARARRFLRAPVSSCRTRDVYLV
                     DMPLSPAELARVRRAFADPVTARAAAGRLPPPKFDWLIEVGFRPGVTDNLGHTAREAI
                     ADVLDREPGPREGVYTATQYFLLGPKLARADAGRIGRDLLANELIQTCRVFSPAEWAA
                     APPDTEPPVIRETVEPRARPVDLGGPDEELLRISREGVLSLDLGEMKAIRDYFARPEV
                     RSAREALGLPAAPTDVEVECLAQTWSEHCKHKIFAARIRYVDVDGREEWIDSLFKTFI
                     RGATERVARERDWLVSVFTDNAGIIRFSDRYHLAVKVETHNTPSALDPYGGAITGIVG
                     VNRDLMGAGMGGELVCNTWGYCFASPFHDGPVPEGLMPPRRIRDGVHQGVIDGGNQSG
                     VPWMRGFERFDERFLGKPLVFCGTVGRIPCALQGRPSERKEVAPGDLIVMCGGRIGKD
                     GIHGATFSSEELRKESPAQAVQIGDPITQRRMYEFLIEARDAGLYRAITDNGAGGLSS
                     SVGEMSRLSGGADLDLALAPLKYEGLQPWEILLSEAQERMTLAVPPEHWDTLSALARR
                     RDVEATALGRFTGSGALVVRYGDRVVGRIGLDFLHDGCPRLTLEARWTPPAVPAPARP
                     RAGARTGVLLDLLRSLNVCSKEFKSRRYDAEVKGLSVVKPFVGARADLPADATVMRVE
                     HDRDEGLILADGINPFYSDLDTYAMAASVVDEAVRRVISAGGSLDRIAGLDNFCWPDP
                     VLSEKTPDGPHKLAQLVRANRALYDVTTAYGVPCISGKDSMKNDSVRGGKKISIPPTL
                     LFSTIGVLDDVRRAVTPDFKQAGDVIYAVGLTRDELGASEYHRLLAARQGRPGAIGGA
                     PPGLDAALATRLYRAIGEAAQAGLLRSSHTPTLGGLAVALALAAMGGNLGADADLAAA
                     PGAAGLDDDALLFSESNSRFVVTCAPERAGELEALFRDLPCARAGTVTAEARLRLRIG
                     SRRKADVSIDRIRRAFKETLDGF"
     gene            5474..6247
                     /locus_tag="PK634_12305"
     CDS             5474..6247
                     /locus_tag="PK634_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOW98560.1"
                     /translation="MVSDPSILILTGHGLNCEAESKYAWERAGARTKLLHFNDLLAEP
                     RSLHDHAGLMFIGGFSYGDHMGSGHVMALRARHRLREDLEQFIGAGKLVLGVCNGFQI
                     LVKLGLLPGLDGDYFTPRAALLPNEGGVFQNFWVRVRFEPGSPCVFTRGLEFMDLPIR
                     HGEGRFFAPPDVLARIEAGGCAACRYVDPATGEPATKFPHNPNGSVNAIAGLCDPTGR
                     VFGLMPHPEANLHRENHPRWTGGDGLMLFQSAVAWLRKA"
     gene            complement(6255..7442)
                     /locus_tag="PK634_12310"
     CDS             complement(6255..7442)
                     /locus_tag="PK634_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98561.1"
                     /translation="MNKWLVTSLALVLAGLRAGSAEEPADPPKPSARRQYSGRVEMHA
                     VTVEATPVTEEEKKIPVLPAEGLAPRRRPDAFLQTDLLRSIPPPLPARPSKENRQDQN
                     WLRPAMLNVKTENEIAFEEERRESGWGWLADDVKERTDALDKTQDNARDEEQETDGLQ
                     DGLSDEATGMLLQQPYDRRIADRVLQEVGVRDAQDLARVDGPEQEPGAAQAKAEDEEG
                     PLRTRVTALDDPAASDRDRDRILSPRDPAISWRGKEKDEPSLMPRTEALFAVPEPAGL
                     DLPEMPKSAEAPSVLLPGAGQPGTRASTGYDWNSATRSASPAAAATPGYGLASPASSL
                     GRAGNLFSAGPVAPPTFGAPPSPSLSSSPGIQAGKPADVLRPWGSMLTPVTPSASGTG
                     SSH"
BASE COUNT         1130 a         2754 c         2525 g         1182 t
ORIGIN      
        1 aaacatcgtc tccaagttcg agaccatgga caaccattgg gaggccgcgc gtctcggcgc
       61 ctccgagatg tccgtcgccg tgctggcttc cgccggcacc aacgtcatcg tgatgctgcc
      121 catcgccatg atgaccagcc tggccgggcg catgctgacc cccttcgcca tcgccaccct
      181 catcgtcaac gccgtctcca tcctcatcag cttcaccctc acccccatcc tggccgcgtt
      241 gctgctggaa cccgcgtcgc ggcgccgcga caatgccttc gcccgtttcg gcaagcggtg
      301 ggaggccggc ttccagaccg tcggcgtcgg gttcagcaac gccctgcgtc gcatcgccgg
      361 gaaaaagcgc tacaacctgc ccgtcgtcgc cggcttcatc ctgttctttt tcttcaccat
      421 gaaattcggc gggagccggc tcggattcag tttcttcgaa acccccgaca tggcccgcgt
      481 cttcatccgc gtcgaaatgc cgccctacta cgacctggaa aagaccgtcg cgcgcatcga
      541 cgaggtccag tcgcgcctgt tggcgttctc cgacctcgag cacgtcctga ccacggccgg
      601 caaggccgaa tccatgagcg gccaggccag cgaaggcgtg tacatgggcc agatcgaact
      661 cttcttcaag cccaagaccg agcgcccgtg gaatctcctc gaccggctgg acgaaatccg
      721 cgcctcgctg aaggacgaaa ccgattgctt cgtctccgcc tccgtccccg gccacatggg
      781 cggccaaagc ttccagatcg accagaacct ttgcggcgag gatctcgacg tgctcgacaa
      841 cacggccatc gccatccgcg acgccgccgc cggcctgccc ggcgtggcca tgatcgagac
      901 gaccgtccgc gacacgaaac ccgaaatccg cgtcctgccc aagcgccccg tgctgtccga
      961 cctcggcctg ccggcccacc tcctcgcctc catcgtccgc gccaacgtgg aaggcctcga
     1021 agccgccgac ttcaagcgcg gcgaccgcac ctacgacatc cgcgtcaagc tcgccgagca
     1081 gcccggcaag gaccagatcc gccaattcct gctccccggc gccgacggcc gccccatccc
     1141 cctcgagacc gtcgccgacg tcgtcgatgc gcgcagcaag gtccagatct accgctacga
     1201 caagcgccgc accgtgaaga tcctcggcga cctcaagccc ggggccacca tgagcggcat
     1261 cagcgacgcc atggcccgca ccatcgcgga ccggaaactc cttcccgcgg gctacaccct
     1321 cggcaacacc ggcatgatcg agatgatgga agacaccatc gccgatttcg gcgaagccat
     1381 cctcctcgcc accttcctga cgctcctcac gctggcggcc atcctcgaat cgtggaaacg
     1441 ccccgcgctc atcctgctca ccttgcccat gggcctgatc ggcatcgtgt ggtcgctcgc
     1501 cctcaccggg aaagccatca ccatgctcgt catgctcggc tcgctgatgc tcatcggcat
     1561 cgtcgtgaac gccgccattc tcatcgtgga caagatggcc cagcacatca aggacggcat
     1621 cagccgccgc gacgcgatgc tcaccgccat ggtcgaggaa ttccgccccg tcctgatggt
     1681 gatcctcgcc tccgggcttg gcatgctgcc catggccatc ggcgccggca tcggttctga
     1741 aaaccgcgcc ggcatcggca tcgcctccgt cgccggcatc atggtggccg gcttcttcac
     1801 catgaccatc ctgccgctga tctacaccct cttcaccgga aagccccggg cctcgcacga
     1861 cgccggctcc cgcggggagc ggaacccccc ggaacccgcc gggccgccgc cacaagcggg
     1921 ttgatgccgc cgcgcgggag gcgtaggatg gcgcggtaaa cgagggaact actatgccgc
     1981 tgaaaaccac cgtcgagaag cgccgccagg gcgggtacct tgtccggctc cagggccggc
     2041 tggacagcga gaccacgccg ggctgcgaga agaaaatcct gccgctgctg gaggaaaagg
     2101 tccacaccgt ggtcctggaa gccgccggcc tggagtacat cagcagcgcc gggctgcgcc
     2161 tcgtgctgga gctacggaag cagatggcgg cgcgcaaggg gcacctggcg ctggcgcacc
     2221 tccagccggc tgtcgcccag gtcttcgagg tcgcggacat cctgccgcgc acggggatct
     2281 tcgattccgt cgagagcgcg gacatgttcc tcgacgccat ccagcgccgg gagcaggtca
     2341 agagcgtcga cgaaaccgac tgaccccgcg gcgccgtcct cccgttttct gacgcaacaa
     2401 agtccttcac acccgtgaag tgatttgttg ccgaagggca cggcggcgcc tatcggcttg
     2461 cctccggcgc gcccggcggg tacaaggcga ggggtcatgg cgatccgcct cgaggtttct
     2521 ttgaatccga gcatcccgga cgcccgcggc cgcgacaccg ccgcccgggc gcgccgcttc
     2581 ctgcgcgcgc ccgtttcgtc ctgccggacc cgcgacgtct acctcgtgga catgcccctc
     2641 tcgccggccg agctcgcccg cgtgcgccgc gccttcgccg atcccgtcac cgcgcgcgcc
     2701 gccgcgggac gcctgccgcc gccgaaattc gactggctga tcgaggtcgg attccggccc
     2761 ggcgtgaccg ataacctcgg ccacacggcc cgcgaagcga tcgccgatgt cctcgaccgc
     2821 gagcccggcc cgcgcgaggg cgtctatacc gccacgcaat attttctgct cggaccgaag
     2881 ctcgcgcgcg ccgacgccgg gcggatcggc cgcgacctgc tggccaacga gctgatccag
     2941 acctgccgcg ttttctcgcc ggccgagtgg gccgccgcgc cgccggacac cgagccgccc
     3001 gtcatccgcg agaccgtcga accgcgggcg cgcccggtcg acctcggcgg cccggatgaa
     3061 gagctgctgc gcatcagccg ggagggcgtg ctgtccctcg acctcggcga gatgaaggcg
     3121 atccgcgact acttcgcccg gccggaagtc cgatccgcgc gagaggcgct cggtctgccc
     3181 gccgcgccga cggacgtcga ggtggaatgc ctggcccaga cctggtccga gcactgcaag
     3241 cacaagatct tcgccgcgcg catccgctac gtggacgtgg acgggcgcga ggaatggatt
     3301 gattcgctct tcaagacgtt catccgcggc gcgacggagc gggtcgcgcg ggagcgggac
     3361 tggctggtct cggtcttcac cgacaacgcg gggatcatcc ggttcagcga tcgctaccac
     3421 ctcgcggtca aggtcgagac gcacaacaca ccgtccgcgc tggacccgta cggcggcgcg
     3481 atcacgggca tcgtcggcgt caaccgcgac ctgatgggcg cgggcatggg cggcgaactg
     3541 gtctgcaaca cgtggggcta ctgcttcgcc tcgcccttcc acgacggccc ggtgccggag
     3601 gggctcatgc ccccgcgccg catccgcgac ggcgtccacc agggcgtgat cgacggcggc
     3661 aaccagagcg gcgtgccgtg gatgcgcggc ttcgagcggt tcgacgagcg cttcctcggc
     3721 aagccgctcg tcttctgcgg gacggtcggc cgcatcccgt gcgcgctgca ggggcgtccc
     3781 tcggagcgta aagaggtcgc gccgggcgac ctgatcgtca tgtgcggggg gcgcatcggc
     3841 aaggacggca tccacggcgc gacgttctcg tccgaggagc tgcggaagga gtcgcccgcg
     3901 caggcggtgc agatcggcga cccgatcacc cagcggcgca tgtatgagtt cctgatcgag
     3961 gcgcgcgacg cggggctgta ccgcgcgatc acggacaacg gcgccggcgg cttgagctcg
     4021 tccgtcggcg agatgagccg cttgagcggc ggcgcggacc tcgacctggc cctcgcgccg
     4081 ctgaaatacg agggcctgca gccctgggaa atcctgctgt ccgaggccca ggagcgcatg
     4141 accctcgcgg tcccgccgga acactgggat accctttcgg ccctcgcccg gcggcgcgac
     4201 gtggaggcga ccgcgctggg ccggttcacc ggcagcggcg cgctggtggt ccggtatgga
     4261 gaccgggtgg tcggccggat cggcctcgat ttcctgcacg acggctgccc gcggctgacc
     4321 ctcgaggccc ggtggacgcc gcccgcggtc cccgcccccg cgcgcccccg ggcgggcgcg
     4381 cggacgggcg ttctgctgga cctccttcgt tcgctgaacg tgtgttcgaa ggagttcaaa
     4441 tcgcgccggt acgacgccga ggtcaagggc ttgagcgtgg tcaagccgtt cgtcggcgcg
     4501 cgcgcggact tgccggcgga cgcgacagtc atgcgcgtcg agcacgaccg cgacgagggc
     4561 cttattctcg ccgacggcat caatcccttt tactccgacc tggacaccta cgccatggcc
     4621 gcctcggtcg tggacgaggc cgtgcgccgc gtcatcagcg cgggcgggtc tctcgaccgg
     4681 atcgcggggc tggacaactt ctgctggccc gacccggtgc tttccgagaa gacgcccgac
     4741 ggcccgcaca agctcgccca gctcgtccgc gcaaaccgcg cgctctacga cgtcacgacg
     4801 gcgtacggcg tgccctgcat ctcgggcaag gacagcatga aaaacgattc cgtgcgcggg
     4861 gggaaaaaga tttccatccc gccgacgctg ctcttctcca cgatcggcgt cctcgacgac
     4921 gtgcgccgcg ccgtgacgcc ggatttcaag caggccggcg atgtcattta cgcggtcggc
     4981 ctgacccgcg acgaactggg cgcgtcggaa taccatcgcc tgctcgccgc gcggcagggc
     5041 cggccgggcg cgatcggcgg cgcgccgccg ggcctggacg ccgcgctcgc gacgcggctc
     5101 taccgcgcca taggcgaggc cgcgcaagcg gggctgctgc gctcgtcgca tacgccgacc
     5161 ctgggcggac tggccgtcgc gctggcgctg gcggcgatgg gcgggaacct cggcgcggac
     5221 gccgacctcg cggccgcgcc cggcgcagcg ggattggacg acgacgcgct gctgttttcg
     5281 gaatcgaaca gccggttcgt ggtcacctgc gcgccggaac gcgccggcga gttggaggcg
     5341 cttttcaggg atttgccgtg cgcgcgggca ggaaccgtga cggcggaggc gcggctgcgg
     5401 ctgcgcatag gcagccggcg caaggcggac gtctccatcg acagaatccg gcgcgcattc
     5461 aaggaaacgc tggatggttt ctgatccctc cattctcatc ctgaccgggc acggactgaa
     5521 ctgcgaggcc gagtcgaagt acgcctggga gcgggccggc gcgcgaacga agctccttca
     5581 tttcaacgac ctgctcgcgg agccccggag cctgcacgac cacgcggggc tgatgttcat
     5641 cggcggattt tcctacggcg accacatggg ctccggccac gtgatggcgc tgcgcgcgcg
     5701 ccaccggctg cgggaggacc tggagcagtt catcggcgcg ggcaagttgg tgctgggggt
     5761 ctgcaacggc ttccagatcc tcgtgaagct cggcctgctg cccgggctgg acggcgacta
     5821 cttcacgccg cgcgccgcgc tgctgcccaa cgagggcggc gtgttccaga atttctgggt
     5881 gcgcgtgcgg ttcgagccgg gctcgccctg cgtgttcacg cgcgggctgg agttcatgga
     5941 cctgccgatc cggcacggag agggccggtt cttcgcgccg ccggacgtcc tcgcgcggat
     6001 cgaggccggc ggatgcgcgg cgtgccgcta cgtcgacccc gccaccggcg agccggccac
     6061 gaagttcccg cataatccca acggctcggt gaacgccatc gcggggctct gcgacccgac
     6121 cggccgcgtg ttcggtctca tgccgcaccc ggaggcgaat ctccaccgcg agaaccatcc
     6181 ccgctggacc ggtggcgacg ggctgatgct cttccagagc gccgtggcgt ggctgcgcaa
     6241 ggcgtgaggc gtgcctagtg cgaggagccc gtcccggacg cggacggcgt gacgggggta
     6301 agcatcgagc cccagggccg cagcacgtcg gccggcttgc cggcctgtat ccccggcgag
     6361 gaggagaggc tcggtgaagg cggcgcgccg aacgtgggcg gggcaaccgg cccggcactg
     6421 aacaggttgc ccgcccggcc caggctggac gccggggagg ccaggccgta gccgggagtg
     6481 gccgcagccg ccggcgacgc cgaccgggtc gccgaattcc agtcgtagcc ggtcgaggcg
     6541 cgtgtcccgg gctggcccgc gcccggcaac aggacggagg gcgcctcggc gcttttgggc
     6601 atctcgggca gatccaaccc cgccggttcc gggacggcaa aaagggcttc ggtcctcggc
     6661 atcagggagg gctcgtcctt ttcctttcca cgccaggaga tggcagggtc gcgcggggaa
     6721 aggatccggt cgcgatcgcg gtccgaagcg gcgggatcgt ccagggcggt cacgcgcgtc
     6781 cgtagcgggc cttcctcgtc ctccgccttg gcctgcgcgg cccccggctc ctgctccgga
     6841 ccatccaccc gcgccaggtc ctgcgcgtcg cgaacgccga cttcctggag cactcgatcg
     6901 gcgatccggc ggtcgtaggg ttgctgcagc agcatccccg tggcctcgtc ggacagcccg
     6961 tcctgcagcc cgtccgtttc ctgctcctcg tcccgggcgt tgtcctgcgt cttgtcgagg
     7021 gcgtcggtcc gctccttcac gtcgtcggcg agccagcccc agccggattc ccggcgctcc
     7081 tcttcgaagg cgatttcgtt ctccgtcttc acgttcagca tcgcgggccg cagccagttc
     7141 tggtcctgcc gattctcctt ggaaggccgc gccggcaagg ggggggggat gctcctgagc
     7201 aagtcggtct gcaagaacgc atcgggccgg cggcggggcg ccaatccttc ggcgggcagg
     7261 accgggatct tcttctcttc ctcggtcacg ggcgtcgcct ccaccgtgac cgcgtgcatc
     7321 tcgacccgcc cggaatattg tcgcctggca gacggcttcg gcggatccgc cggctcttcc
     7381 gcggaccccg cgcgcagccc ggccaggacc agcgccaggc ttgtgacgag ccacttgttc
     7441 acgcttccat tgtagggcgg cggttcggcg gcgtcaatgg agcgccccct tgcagccccg
     7501 cgtctttctg ataggtttcc cgctatgaca tcccgcgccg tctgcctgtt catgctgctg
     7561 gggctcgcgg cgccggtttc cgccgcggag c
//
LOCUS       DAOOTK010000136         7586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6042_length_7586_cov_4.457841, whole genome shotgun sequence.
ACCESSION   DAOOTK010000136 DAOOTK010000000
VERSION     DAOOTK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_7586_cov_4.457841"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PK634_12315"
     CDS             <1..1083
                     /locus_tag="PK634_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOW98562.1"
                     /translation="PYNDRGWSQAHYDAGKYVEARLPGAQMIYVDKVNPGDRPGTTPA
                     QLAEDLVSRGARLVIFNSDDMKDSCLDFAKAHPDIFVIHASGDNAWKEGKDYKGLPNL
                     VNIMGRMEYGKMMAGFAAALATQTGRIGYLGPLINDETRRLTASAFLGARYAWTKYRG
                     RDPAELMFKVTWIGFWFNLPGVTADPTQVADDFYNSGFDVVLSGIDTTEALVEARKMA
                     GTGRKVFAVPYNYVGACETAPEVCLGVPYFNWGPAYLRHLRAAREGGWQAAWEWNGPD
                     WADINDPDRSAVGFAKGRALSPELSVALDQFAGELAGGLALWSGPLTLQDGAEYLRDG
                     ERATDRQVWYLPRLLEGMEGQSVAGP"
     gene            1106..2566
                     /locus_tag="PK634_12320"
     CDS             1106..2566
                     /locus_tag="PK634_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW98563.1"
                     /translation="MNIALENISKRFGEVQANEGISLAVPAGTIHGILGENGAGKSTL
                     MKILSGFIRADSGRTFLDGAPAQIRGPADAIRLGIGMLHQDPLDFPPMTAVDNFLAGR
                     TGAWRLSRPDARRELEERQADFDFRVDPGARVENLTVGERQQLEMVRLLSLGARALIL
                     DEPTTGISALQKDRLFAALRKLAADGKSIILISHKLRDVEMLCDEVTVLRRARVAGRA
                     QAPYDLNELVRLMFGRVVTREPRLEVPTGGPLLTLDGLAVEDHRLRLAGLNLEIRAGE
                     VIGLAGLEGSGQELFLRACGGLLRPAAGRLRLAGRDLAGEPYPVFRDAGIAYVPAARL
                     EEGLVAGLTPAEHFAIVEPGGGLVIDWKLTMEQAERRIAEYDIRGAATGPVDSLSGGN
                     QQRALLSLLPPGLCLVLLEHPTRGLDVESASAIWRKLLDRCADGAALVFTSSDLEEIL
                     QYSDRVLVFFGGQVAGPVPASAMTVERLGEMMGGQP"
     gene            2563..3612
                     /locus_tag="PK634_12325"
     CDS             2563..3612
                     /locus_tag="PK634_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW98564.1"
                     /translation="MKEGRPGWEAAVRIGLAAGLAVVFVSGLLALLGVSPARAFMEMA
                     SGSVGSAGKLADVLVAWVPLLLVASGLLVTFAAGLWNIGIEGQVMMGAVFTTWMLRLL
                     QDAALPPALVLALAIGAGMLGGALWAVFAGLLKTHGGVNEIFGGLGLNFVASGLALWL
                     IFGPWKRPGVGSMSGTEPFPEALWLPQIGALRLSGWALALGVLGIVLVGALLRGTYFG
                     LKVKAVGRNARAAFLLGIPTTRYMLSGFALCGALAGAAGALQVTAVYHRLIPAISSGY
                     GFLGLLVAMLSGYRALAVPFVAFFFAALNIGSIQLPIVFRLDSSLSGVLQGMLVLCVL
                     VVHGARRRFRREAAP"
     gene            3609..4610
                     /locus_tag="PK634_12330"
     CDS             3609..4610
                     /locus_tag="PK634_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW98565.1"
                     /translation="MNGESLVLVGLAGILSSATPLLFAAIGETLAERAGVINLSLNGT
                     ILLSAMGSFGVAVKTGSLALGFLAGAVIGAFMALIVAFGSITLRQSQVAVGFVLAMVG
                     RDLSYFLGAPLMGRPGPTVPSWPVPALGDIPVLGPLLFRHDLPTYAGFLLVAGAWFWL
                     YRTRPGLILQGVGERPAAAHARGANVNRLRYLYTIFGGALVGLAGPIYSLSIKAGWMG
                     TISGLDGLGWIALAITIFGGWNPWRAALGAWFFALLQWLGLVLQPHLPGVPMQVLQVA
                     PFPLMILALLLVNAGNAEWVQRALAALSEPRRRAAARLLRSLRAAPPASLGEHFESR"
     gene            complement(4934..6652)
                     /locus_tag="PK634_12335"
     CDS             complement(4934..6652)
                     /locus_tag="PK634_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98566.1"
                     /translation="MTPLARQGDVAPGTEDGVTYLHFMNPLINSSGKVSFVASLQGPG
                     IGFSNIVGLWSQKISAFELVARPLNPAPGCEEGVLFWGTLGFTTTPWPTFNDRSEAAL
                     RTNVNGPGVGSTNDSGIWAGTAATFDLAGREGSDAAVPGESRTFYAFDVPGMGNGGHC
                     VFQARLDYGTVGGLSHDYGIWSRTSTGLQVIVVSGDSVPGSLGVFEEFHTPAANRSGQ
                     IAFRASLYGVTDDQGIWSYANGTGTHIALAGNQAPGAPDGALFNQYHPMGDPVINDAG
                     DVVFGARLRLGGTSETNDSGLWMWHGGELQALALEGQLVPGAEAGTLFAHFDPAERPT
                     LGGAHAAFRASLTGDTVSVTNDTGIWMGTPSNVVCVVREGDVAPAAGADVYFGAFPYN
                     STYFSINSNGTVAFFSGLTGAGVSSSNNTALWIWNPGDGSYSLVTREGNPLEVAPGDE
                     RPIIELKLFGGGGQDGRGTSLNDAGQIAFYAKCTNDFSTVCLAEPGGAATPDLEWGRL
                     QGGGIVLQWKTMGAGDVYRVERSASLTEPDWQLPEPTNEWPIGVNAWTGTPTAAGFYR
                     VSSSSD"
     gene            7247..7459
                     /locus_tag="PK634_12340"
     CDS             7247..7459
                     /locus_tag="PK634_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98567.1"
                     /translation="MAVKKVWFLLVGAWDLVAYGYPRAIMDVDIWELLAQENADAVWR
                     ALRRFGAVETLEELANSEHEAPGDLR"
BASE COUNT         1179 a         2485 c         2596 g         1326 t
ORIGIN      
        1 ccctacaacg accggggctg gagccaggcg cactacgacg cggggaaata cgtggaagcc
       61 cgtctgcccg gggcgcagat gatctatgtg gacaaggtga atcccggcga ccggccgggc
      121 acgaccccgg cgcaactggc cgaggacctg gtttccaggg gcgcgcgcct cgtgatcttc
      181 aactcggacg acatgaagga cagctgcctg gatttcgcga aggcgcatcc cgatatattc
      241 gtcatccacg cctccggcga caacgcgtgg aaggagggca aggactacaa gggcctgccc
      301 aacctcgtga acatcatggg ccgcatggag tacgggaaga tgatggccgg gttcgccgcc
      361 gcgctcgcga cccagaccgg ccgcatcggc tatctcggcc ccctcatcaa cgacgagacc
      421 cgccgcctga ccgcctccgc gttcctgggc gcgcggtacg cgtggacgaa gtatcgcggc
      481 cgcgatccgg ccgaactgat gttcaaggtc acctggatcg gattctggtt caacctcccc
      541 ggcgtcaccg ccgatccgac gcaggtggcc gacgatttct acaatagcgg cttcgacgtg
      601 gttctttcgg gcatcgacac gaccgaggcc ctggtcgaag cccgcaagat ggccggcacg
      661 ggccgcaagg tgttcgccgt gccctacaat tacgtcggcg cctgcgagac ggcgccggag
      721 gtgtgcctgg gcgtcccgta cttcaactgg gggccggcgt acctccggca tctgcgcgcc
      781 gcccgggagg gcgggtggca ggcggcctgg gagtggaacg gcccggattg ggccgacatc
      841 aacgatccgg atcggtccgc cgtcggcttt gccaagggcc gcgcgctctc cccggaactc
      901 tccgtggcgc tggaccagtt cgccggggaa ctggccggcg ggctggcgct gtggagcggg
      961 ccactcacgc tgcaggacgg cgcggagtac ttgcgcgacg gcgagcgggc gaccgatcgc
     1021 caggtgtggt acctgccccg attgctcgaa gggatggaag gacaaagcgt cgctggccct
     1081 tgaccgggcg ccggcggccc gaccgatgaa tatcgcgctg gagaacatca gcaagcgctt
     1141 cggcgaggtc caggccaacg agggcatctc cctggccgtc cccgccggaa ccatccacgg
     1201 catccttggc gagaacggcg ctggcaagag cacgctgatg aagatcctgt cggggttcat
     1261 ccgcgccgac tcgggacgca ctttcctgga cggcgcaccc gcgcagatcc gcggtccggc
     1321 tgacgcgatc cgcctgggca tcgggatgct gcaccaggat ccgctggatt ttccgccgat
     1381 gacggcggtg gataactttc tcgccggccg gaccggcgcg tggcgattgt cccgcccgga
     1441 cgcgcgccgg gagctggagg agcgccaggc ggacttcgac tttcgggtgg atcccggagc
     1501 gcgcgtggaa aacctgaccg tgggcgaacg ccagcagttg gaaatggtgc gcctgctctc
     1561 cctgggcgcg cgggcgctga tcctggacga gcccaccacc ggaatcagcg ccctgcagaa
     1621 ggaccgtctt ttcgccgcgt tgcggaaact ggccgccgac gggaagagca tcatcctgat
     1681 ctcgcacaag ctgcgcgacg tggaaatgct ctgcgacgag gtgaccgtcc tgcgccgggc
     1741 gcgggtggcg ggccgggccc aggcgcccta cgatctgaac gagttggtcc ggttgatgtt
     1801 cgggcgggtc gtgacccggg aaccgcggct ggaggtcccg accggcggac cgttgctgac
     1861 gctcgacggg ttggcggtgg aggaccaccg cctgcggctg gccgggctca acctggaaat
     1921 ccgcgccgga gaagtcatcg ggctggcggg actggagggc agcggccagg agctgttcct
     1981 gcgggcctgc ggcgggttgc tccgccccgc ggccggccgc ctgcggctgg ccggtcgcga
     2041 cctggccggc gaaccgtacc cggttttccg cgacgcgggc atcgcctacg tgccggcggc
     2101 gcggctggag gaaggcctgg tggccggact tacaccggcg gagcatttcg cgatcgtcga
     2161 gcccggcggc ggtcttgtga tcgactggaa gctgacgatg gagcaggccg agcggcggat
     2221 cgcggaatac gacatccgcg gcgcggcgac gggtcccgtg gattccctct ccggcggcaa
     2281 ccagcagcgc gccctgctgt ccttgttgcc gcccggcctt tgccttgtcc tgctggagca
     2341 cccgacgcgg ggcctggatg tggaatcggc atcggccatc tggcgcaagc tcctggatcg
     2401 ctgcgcggac ggcgcggcgc tggtattcac gtcctccgac ctcgaggaaa tcctccagta
     2461 cagcgaccgc gtgctcgtgt ttttcggcgg gcaggtggcc gggccggtgc cggcgtcggc
     2521 gatgaccgtg gagcggctcg gtgaaatgat gggagggcag ccgtgaagga gggacgccca
     2581 ggatgggaag ctgcggtccg gatcgggctg gccgccgggc tggccgtggt cttcgtgtcc
     2641 gggctgctcg cgctcctcgg ggtgtcgccg gcccgggctt tcatggagat ggcctccggg
     2701 tcggtcggct cggcgggcaa gctggccgac gtgctcgtgg cgtgggtgcc gctgctcctg
     2761 gtcgcgagcg ggctgctggt gacgtttgcc gccgggctct ggaacatcgg catcgagggg
     2821 caggtgatga tgggcgccgt cttcacgacc tggatgctgc gcctcctgca ggacgccgcg
     2881 ctgccgcccg cgctggtcct ggcgctggcg atcggggccg gcatgctggg cggggcgctg
     2941 tgggccgtct tcgcgggact attgaagacc cacggcgggg tgaacgaaat tttcggcggg
     3001 ctggggctga acttcgtcgc cagcggcctg gcgctctggc tgatcttcgg accgtggaag
     3061 cggccggggg tcggttccat gagcggcacc gagccgtttc ccgaggcctt gtggctgccc
     3121 cagatcggcg cgcttcgctt gagcgggtgg gccctggcgc tcggcgtcct cggcatcgtg
     3181 ctggtcgggg cgctgctgcg cggcacctac ttcggcctca aggtgaaggc cgtcggccgc
     3241 aacgcccggg ccgcgttcct gctgggcatc ccgacgacgc gctacatgct gtccggcttc
     3301 gcgctctgcg gcgcgctcgc gggggcggcc ggggcgctcc aggtcacggc cgtctaccac
     3361 cggctgatcc ccgccatctc cagcggctac gggttcctgg gcctgctggt cgccatgctg
     3421 tccggctacc gggcgctcgc cgtgcccttc gtcgccttct tcttcgccgc gctcaacatc
     3481 gggagcatcc agttgcccat cgtcttccga ctcgactcgt ccctgtcggg cgtcctgcag
     3541 ggcatgctgg tcctgtgcgt cctggtggtc cacggcgcgc ggcggcggtt ccgccgggag
     3601 gcggcgccat gaacggcgaa tccctggtcc tggtcggcct cgcgggcatc ctctcgtccg
     3661 ccacgccgct gctcttcgcg gcgatcggcg agacgctggc cgagcgcgcc ggcgtgatca
     3721 acctctccct gaacgggaca atccttctct cggcgatggg cagtttcggg gtcgcggtga
     3781 agacgggcag cctggcgctc ggcttcctgg ccggcgcggt catcggggct ttcatggccc
     3841 tcatcgtcgc gttcggcagc atcacgcttc gccagtcgca ggtggccgtc ggcttcgttc
     3901 tcgcgatggt cggccgggac ctctcctatt tcctgggcgc cccgctcatg ggccggccgg
     3961 gcccgacggt gccgtcgtgg ccggtccccg cgctgggcga tatacccgtg ctcggcccgc
     4021 tgctcttccg gcacgacctc ccgacgtatg ccggcttcct gctcgtcgcg ggcgcgtggt
     4081 tctggctgta ccgcacccgg cccggcttga tcctccaggg ggtgggggag cggccggcgg
     4141 ccgcgcacgc gcgcggggcg aacgtgaacc ggctccgcta tctgtacacg atcttcggcg
     4201 gcgcgctcgt gggcctcgcc gggccgatct actcgttgag catcaaggcc ggctggatgg
     4261 gcaccatctc cgggctcgac ggcctcggct ggatcgcgct ggccatcacg atcttcggcg
     4321 gctggaaccc gtggcgcgcc gcgctcgggg cctggttctt cgccttgttg cagtggctcg
     4381 ggctggtgct gcaaccgcac ctgccgggcg tccccatgca ggtcctccag gtcgcgccgt
     4441 tccccctgat gatcctggcg ctgctcctgg tcaacgccgg gaacgccgag tgggtgcagc
     4501 gcgccctggc cgccctctcc gaaccgcgcc gccgcgccgc cgcgcgcctc ctgcgctccc
     4561 tccgcgccgc cccgcccgcc tcgctcggcg agcacttcga gtcacggtaa ctgacgccgt
     4621 ggcaagcgat cgggcgacaa ttatttgttt cttgaagctg accccaaatc cgactgagct
     4681 gttcgccgat ttgatccgag acgcctgaag gcctagcccg cttattgcat gagcgtagag
     4741 gcactcacgc aattcgcgtg ccagaagacg tgacgttttg tcgaatcaag gattagacaa
     4801 aacgagcgta gcaagccccg tttgtgcccg taacgcgggc gcatcatcgc gtgaccgcgt
     4861 tgaggcgcgg tacgcgactc tcacgcggcg gccctttggg cggctaatgc gcgccaatcg
     4921 cgcattagcc gggctagtcc gaggatgacg acacgcggta gaaaccggcg gcggtcgggg
     4981 tgccggtcca agcgttgacc ccgatgggcc actcgttggt gggctccggc aactgccagt
     5041 cgggttcggt gagggaagca gatcgttcaa cgcggtagac atcaccggcc cccatggttt
     5101 tccactgcag gacgatcccg cccccctgga gacggcccca ttcaaggtcg ggcgtggcgg
     5161 cgccgccggg ctcggccagg cagacggtgc tgaaatcgtt ggtgcacttg gcgtagaacg
     5221 cgatctggcc ggcgtcgttc agactggtac cgcggccgtc ctggccgccg ccgccgaaaa
     5281 gcttcagctc gatgatcggc cgttcgtcgc cgggcgcgac ctccaggggg ttgccttccc
     5341 gggtgacgag ggaataggat ccgtccccgg gattccagat ccacagggcg gtgttgttcg
     5401 aactgctgac tccggcaccg gtcagacccg aaaagaaggc cacggtcccg ttggagttga
     5461 tactgaaata cgtcgaatta tacgggaatg cgccgaagta cacgtccgcg ccggccgccg
     5521 gcgccacgtc gccctcccgc acgacacaga ccacgttgct cggggtgccc atccagatcc
     5581 cggtgtcgtt ggtcacagag accgtgtcgc cggtgaggct ggcccggaag gcggcatggg
     5641 ctccgccgag cgtcgggcgt tccgcgggat cgaagtgcgc gaagagcgtg ccggcctccg
     5701 caccgggaac gagttggcct tcaagggcca gtgcctgcag ttctcctccg tgccacatcc
     5761 acaggcccga gtcgttggtc tcggatgtcc cgcccaacct caggcgggcg ccaaagacca
     5821 cgtccccggc gtcgttgata accgggtcac ccatggggtg gtattggttg aaaagcgcgc
     5881 cgtccggggc gccgggcgcc tggttacctg cgagcgcgat atgggtgcct gttccattgg
     5941 cataggacca gattccttgg tcatccgtca ccccatagag agaagcacgg aaggcgatct
     6001 gtccgcttcg atttgcggcg ggcgtgtgga attcttcgaa gacgcccaga ctgcccggga
     6061 ctgaatcgcc ggacacaacg atgacctgga gccccgtgga agtccggctc cagatcccgt
     6121 aatcatgcga tagtccgccc accgttccgt agtcgagcct ggcttggaac acgcagtgtc
     6181 cgccgttgcc catgcccggc acgtcgaagg cgtagaaggt gcgtgactcg ccgggaactg
     6241 cggcatcaga accctcccgc ccggccaggt cgaaggtcgc cgcggtcccg gcccatatgc
     6301 cggagtcgtt cgtggagccc acgccgggcc cgttcacatt ggtgcgcagg gccgcctcgc
     6361 tgcggtcgtt gaaggttggc cagggcgtgg tggtgaatcc cagtgtgccc cagaacagca
     6421 ctccctcctc gcaccccgga gcgggattca acggccgggc cacgagctca aaagcagaaa
     6481 ttttttgaga ccacaacccg acgatattgg agaatccaat cccgggcccc tgcagcgaag
     6541 cgacgaaaga aacctttccg ctgctgttga taagaggatt catgaaatgc aggtacgtca
     6601 cgccgtcttc ggttcccggc gccacgtcgc cctgcctggc gaggggcgtc acggtccatg
     6661 ccgcgcccgc cgatgtcacc gcgcccaaag acaacagcag gacagtgcgt gcgattcctc
     6721 ggtttgggcg tttcctcatg attgcccctc tagataaaca tggcccatac ggagattggg
     6781 cgtcaagcag aaccagccgg aaagccgaaa ccaaatcatg gtcaatcgat taggggggcg
     6841 attaggggtc ggattccaaa tattcaattt acggcaagac gcgcatgatt aacgttgcga
     6901 tgcgcgcatg caggcgggcc ccgctcgttc gcaccttgcg cctcatcatc gccaccgcgg
     6961 atgagctgat gccgccatag taatgcctga tctcccgctg tgtcctgtcg gtcaaccgag
     7021 ccgatatttc aacggccgcc accttggcct ctcccgctat atggccgtgc aacttcaacc
     7081 gatccgcggt aaacgacacc gcagcaacca atgcggggac ggttcttctg gggctgaccc
     7141 aactacctaa aaaaacccgg gaagaaaagt ctctcttccg ccgacagggt atgtgaaggg
     7201 gtaaaaacac acggaattca ggggggatcc agacctgttg caggccttgg ccgtcaagaa
     7261 ggtttggttc cttctggtcg gcgcctggga ccttgtcgcc tacggctatc ctcgagcaat
     7321 tatggatgtc gacatctggg aactgcttgc acaggagaat gccgatgccg tgtggcgcgc
     7381 tttgcgtcga ttcggcgcgg tggaaacttt agaggaactg gccaattccg aacacgaggc
     7441 ccccggcgac ttgcggtgac gggggcggcc agcaagtcgg aaaggaggtg gggccagcct
     7501 caaaatcaat aaatccaaaa gcttatttct ggcacccaaa acaccatgca aatgcgaggg
     7561 gggatgtatg atgagaccat cctttc
//
LOCUS       DAOOTK010000137         7580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6047_length_7580_cov_4.623654, whole genome shotgun sequence.
ACCESSION   DAOOTK010000137 DAOOTK010000000
VERSION     DAOOTK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6047_length_7580_cov_4.623654"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK634_12345"
     CDS             complement(<1..329)
                     /locus_tag="PK634_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98568.1"
                     /translation="MLLLAVMLGLLNLLLLGGLLALHHRQRRLARALAEARLAFERWA
                     GGDLDARMVPRDGDPPGQLAAAFNGMAAHLAEQIRLLEQQRDQQEAVFVSMVEGVVAL
                     DNGGRILR"
     gene            complement(337..1023)
                     /locus_tag="PK634_12350"
     CDS             complement(337..1023)
                     /locus_tag="PK634_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOW98569.1"
                     /translation="MAREHVLVVEDEEDIQELIRYNLAKDGFHVTVVPSSEDAFKRIK
                     AAPPAVILLDLMLPGMNGLEFCKLIKGAPSTRGIPVIMVTARGEEADVVAGLELGADD
                     YLTKPFSPRVLLARLRAVLRRRNAAEDDRDAPVHLRDLTIHPGRHEVKLKNKTIELSF
                     TEFRLLQVLARRPGWVLTRQQIVDAVRGPDYPVTERSVDVQVVGLRKKLGAAGDYIET
                     VRGVGYRFRE"
     gene            complement(1102..2547)
                     /locus_tag="PK634_12355"
     CDS             complement(1102..2547)
                     /locus_tag="PK634_12355"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOW98570.1"
                     /translation="MTAPAPALAWFRRDLRIADNPALRTACRSGPVLPVYIHAPEEDG
                     SWRPGAASNWWLHHSLSALRDELRKRGSDLVLRAGRDALSTLLALARETGARTVCWNA
                     VPEPGPRRRNRLVRAALEKEGLTVHLHEASLLFDAPAIRNKQGRPFQVFTPFWRHCTE
                     NLAPGLPEPAPPVLPRPRGFPSSVELESLGLLPSVDWAAGFLETWRPGEAGARRALRR
                     FVREALETYDAGRDRPDRPGTSLLSPHLHFGEISVRQVWRAIETAAASPSCRKASLVY
                     RRELGWREFAHHLLEHFPNTPDRPLHPEFDRLPWRRDPQRLKAWQQGRTGYPIVDAGM
                     RQLWRTGWMHNRVRMIAASFLVKDLLLPWQEGARWFWDTLVDADLANNTLGWQWSAGC
                     GADAAPYFRIFNPVLQAAKFDPEGEYIRRWVPELKALPAPWLFQPWEAEPLDLQAAGI
                     VLGQTYPLPIVDHGEARDRALAAYAVMKHAD"
     gene            complement(2544..4508)
                     /locus_tag="PK634_12360"
     CDS             complement(2544..4508)
                     /locus_tag="PK634_12360"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOW98571.1"
                     /translation="MDAASLSPDEALRLFESGPTVMFKWKAAEGWPVEYVSPNVEQFG
                     FRPDDFVSGRLPYASIVHPDDLARVAEEVRQFSEAGATHFEQEYRIMGKGGRAHWLHD
                     FTVVRREAGGAITHYYGYIEDVTARRQAQEAAQEHWAKYRAIVDAYDGLIYICSADFK
                     IEFMNRRLIERTGRDTTGEPCFRALHDRGAKCPWCVNDRVFQGKTVRWEVLSPKDNRW
                     YYVVDTPIVHPDGRQSKMAMIQDITERKQAETLLRRREAVLEAIGFASEQFLKCHSLE
                     HCLQDVLARLGQALNVNRVHVTEVRASGPDEFLAERRHEWAAAGLPPQIYYSAWRAFP
                     MRRQGFARWIEQLSRGQTVSGDVVEFPSGEQAVLGRQQIGSLAALPYFVGGTWRGFLG
                     FEVSGGRRTWPLVELEALKTVASLIGAAIQRQEAEHTLRRSEERHAHLLNAMPDLMLR
                     FDPLFVLLDIKAAPDGDLPAREDAVGRSLEVVLPASAYAAFRGAADRLWKTGQRQVFD
                     ASLSDDGSRIYEVRMVPAAKGEALAILRDITARKKADVAIAKLAAFPLNNPNPLLELT
                     ESADVLFANQAARDLAAALALGRPSDILPPGYEAVCRECLASGAVRQGLLVRKGGRTI
                     LWMFMPISESRTVHAYGFTPPAGFDLRASA"
     gene            4673..5428
                     /locus_tag="PK634_12365"
     CDS             4673..5428
                     /locus_tag="PK634_12365"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOW98572.1"
                     /translation="MARILTDETSLHRKRDSLRKGARQLQSLVRYELGGAVVLLAAGA
                     LAYKWWGPGLLVVGVAALFVSAGHWMKSKENKDEEGSASAGLQGEQNVTRLLRDGLPN
                     DSYIFNDITLRRGLRSAQMDHVVVSPRGIFVVETKNWRGLISGDEQEAQWTQVKRPGE
                     KPMRRANPILQNRRQINVMQAVLKAGGIDWPDIFPVLISNSRNAQFEIHNRSVPILTR
                     FDAVEYMVGQAPSRQYTEAEVTAVIELLMKKAK"
     gene            5471..6646
                     /locus_tag="PK634_12370"
     CDS             5471..6646
                     /locus_tag="PK634_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOW98573.1"
                     /translation="MAQTAKFDEFRGLSEQPSSPPPKKHWGMLVLSLVGVALVWYLLF
                     WSRAPETEVIEAVVVGEEAERVPINHAEVTDASTDRGAVAREGYRYKVRIEDESSDGV
                     AGVTHIGGLVTFVPDARPGEMVIIEITRLKERTADGMVVKRLPEETIAWTPPPPRPTR
                     PPMFTPDGVYTSVVLELGKKGDAITKINGKVVFLPGAMPGDRVTFRVVEERDSAATGE
                     VLTREPGPVPAPEAFERPRPAMTRPPMFTPDGVYTSVVLEIGKKGDAITKINGKVVFL
                     PGAMPGDRVTFRVVEERDSAATGEVLTREPGPVPAPEAFNRPQPAMTRPPMFQPDAVY
                     TGKVTDIGKKGDALLKVDGKVVFLPGAQLGDEVVFRVVSERDSTAQGEVISRTPAAP"
     gene            6736..7422
                     /locus_tag="PK634_12375"
     CDS             6736..7422
                     /locus_tag="PK634_12375"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOW98574.1"
                     /translation="MLRWVRWLELAGALVLLAGGIGLWVWRDIHWLALVGAGLLFAWL
                     GHHFKMQENSLDAWTIGVGLEGEQGVTDRLATGLDDRYLVLNDVFIQFGRRRAQIDHV
                     VAGPNGIFVIETKNWAGRIVGDETQNHWVFYAAGKDGQELFNPILQSQRQAKFVARRL
                     REAGMDWPDMRALVVMFSEDSRWAIRNQTAPILRPAELAPFIRNAASGRSYRPEEIDA
                     AGRWIMQDSG"
BASE COUNT         1289 a         2394 c         2708 g         1189 t
ORIGIN      
        1 cgcaggattc ggcccccgtt gtccagcgcc accacgcctt cgaccatgct gacgaatacc
       61 gcctcctgct ggtcgcgctg ctgctccagc aggcggatct gttccgccag gtgggcggcc
      121 atgccgttga aggccgccgc cagttgcccc ggcgggtcgc cgtcccgggg gaccatgcgc
      181 gcgtccaggt cgccgccggc ccaccgctcg aacgccagcc gggcctcggc cagggcacgg
      241 gccaaccggc gctgccggtg atgcaaggcg agcaatcctc ccagcagcag gagattcaac
      301 aggcccagca tgaccgcgag aaggagcatg atgcctctat tccctgaacc ggtatcccac
      361 gccgcgcacg gtctcgatgt agtcgcccgc ggcgccgagc ttcttgcgga ggccgaccac
      421 ctggacgtcc accgagcgct cggtcacggg gtagtccggc ccgcggacgg cgtccacgat
      481 ctgctgacgg gtcaagaccc agccgggccg gcgcgccagc acctggagga gacggaattc
      541 agtgaacgac agctcgatcg tcttgttctt cagcttcacc tcgtggcggc ccggatggat
      601 ggtaaggtcg cggaggtgaa cgggcgcgtc ccggtcgtcc tcggcagcgt tgcggcggcg
      661 aaggacggcg cgcaaccgcg ccagcagcac gcgggggctg aacggcttgg tcaggtagtc
      721 gtcggcgccc agctccaggc ctgcaaccac gtcggcttcc tcgccgcgcg ccgtgaccat
      781 gatcaccggg attccgcggg tggacggggc gcccttgatc agcttgcaga attccaggcc
      841 gttcatgccg ggaagcatga gatccagcag gatcaccgcc ggcggcgcgg ccttgatccg
      901 tttgaacgcg tcctcgctgc tggggacgac ggtgacatgg aagccgtcct tggcgaggtt
      961 gtaccggatc agttcctgga tgtcttcttc gtcttccacc accagcacgt gttcccgggc
     1021 catgaatgag actccttttt gccccccgac actctccgcc caccatgtta ggcgacggtg
     1081 agcgcgacgc aaaacctttg ttcaatccgc gtgcttcatg accgcgtagg cggccaacgc
     1141 ccggtcgcgg gcctcgccat gatccacgat gggcaaggga taggtttgtc ccaggacaat
     1201 cccggcggcc tggaggtcga gcggctccgc ctcccagggc tggaagagcc acggggcggg
     1261 aagggccttg agttcgggca cccagcggcg gatgtattcg ccctccgggt cgaatttcgc
     1321 ggcctggagc acggggttga agatccggaa ataaggcgcc gcgtccgccc cgcaaccggc
     1381 ggaccactgc cagcccagcg tgttgttggc caggtccgcg tccaccagcg tgtcccagaa
     1441 ccagcgcgcg ccctcctgcc agggcaggag aaggtccttg acgaggaacg aggcggcgat
     1501 catccggacg cggttgtgca tccatcccgt ccgccagagc tggcgcatcc cggcgtcgac
     1561 gatgggatag ccggttcgtc cctgctgcca cgccttcaac cgctgcgggt cgcggcgcca
     1621 gggcagccgg tcgaattccg gatgcagcgg gcggtccggc gtattcggga agtgctccag
     1681 caggtgatgc gcaaactcgc gccatcccag ctcccgccga tacacgagag aagctttccg
     1741 gcaggagggg gaggcggcag ccgtctcgat ggcccgccac acctgccgga cgctgatctc
     1801 cccgaaatgc aaatgggggg agaggagaga cgttccaggc cgatccggcc ggtcgcggcc
     1861 cgcatcgtag gtttcgaggg cctcccgcac gaatcggcgg agcgcccggc gggcgccggc
     1921 ctcgccgggc cgccaggtct cgagaaaacc cgccgcccag tccacgcttg gaagcaggcc
     1981 caacgattcc agttcaaccg aggaagggaa cccgcggggg cgggggagaa ccgggggcgc
     2041 cggttcggga agcccgggcg cgaggttttc ggtgcaatgc cgccagaagg gcgtgaacac
     2101 ttggaagggc cggccctgct tgttgcggat ggccggcgca tcgaatagca gggacgcctc
     2161 gtgcaagtga acggtcaggc cttctttttc cagcgccgcg cgaacaagcc tgttccggcg
     2221 ccgggggccg ggctccggca cggcgttcca gcagaccgtc cgggctcccg tttcgcgggc
     2281 cagcgcgagg agcgtcgaga gggcgtcccg gccggcgcgg agaacaaggt ccgagccccg
     2341 cttccggagt tcatcgcgca gggcggacag ggagtgatgg agccaccagt tggaggcggc
     2401 gccgggccgc caggagccgt cttcctccgg ggcgtggatg tacacgggca gcacggggcc
     2461 ggagcggcag gccgtcctca aggcgggatt atcggcgatg cgcaggtcgc ggcggaacca
     2521 ggcgagcgct ggcgcgggcg cggtcacgcg gaggcccgga gatcgaagcc ggcggggggc
     2581 gtaaacccgt aggcgtgcac ggtgcggctc tccgagatcg gcatgaacat ccagaggatc
     2641 gttcgtccgc ccttccggac caggagaccc tgccgcacgg cgccggatgc caggcattcg
     2701 cggcatacgg cctcgtagcc cggcggcagg atgtcggagg gacggcccag cgccagcgcg
     2761 gcggcgaggt cccgcgccgc ctggttggca aacagcacgt cggcggattc ggtgagctcg
     2821 agcaggggat tgggattgtt gagcggaaag gcggccagct tggcgatggc gacatcggcc
     2881 ttcttgcggg ccgtgatgtc gcgcaggatg gccagcgcct cgcccttggc cgcggggacc
     2941 atgcgcacct cgtagatgcg cgagccgtcg tcggacagcg aggcgtcgaa tacctggcgc
     3001 tgcccggtct tccaaaggcg atccgcggcg ccccggaacg cggcgtatgc cgaggccggg
     3061 agaacgacct ccagcgaccg gccgacggcg tcttcgcgcg cgggcagatc gccgtcgggg
     3121 gcggccttga tgtccagcag cacgaacagc gggtcgaacc gcagcatcag gtccggcatg
     3181 gcgttcagca ggtgggcgtg ccgctcctcg ctccggcgca gcgtatgctc ggcttcctgg
     3241 cgctggatgg ccgcgccgat caggctcgcc acggtcttca gggcttccag ttccacgagc
     3301 ggccaggtgc ggcgcccgcc gctcacctcg aagccgagga agcccctcca tgtcccgccg
     3361 acaaagtaag ggagcgcggc gagcgacccg atctgctgcc ggcccagcac ggcctgttcg
     3421 ccggaaggaa attccacgac gtcgccgctg acggtctgcc cccgcgaaag ctgctcgatc
     3481 cagcgcgcga atccctggcg gcgcatgggg aaggcccgcc acgcggagta gtagatctgc
     3541 ggaggcaggc cggcggccgc ccattcgtgg cggcgctccg ccaggaactc atccggcccc
     3601 gaggcgcgca cctcggtgac gtgcacgcgg ttgacgttga gggcctgtcc gagccgggcg
     3661 agaacatcct ggaggcaatg ctccaggctg tgacatttga ggaactgttc ggaggcgaag
     3721 ccgatggcct cgaggacggc ttcccgacgg cgcaggagcg tttcggcctg cttgcgctcg
     3781 gtgatgtcct ggatcattgc catcttggac tggcgcccgt cgggatggac gatgggcgtg
     3841 tcgaccacgt agtaccagcg gttgtccttg gggctcaaca cctcccagcg gaccgtcttg
     3901 ccctggaaga cgcggtcgtt gacgcaccag gggcatttcg cgccgcggtc gtgcagggcc
     3961 cggaagcacg gttcgcccgt ggtgtcgcgg ccggtgcgct cgatgagccg gcgattcatg
     4021 aactcgatct tgaagtcggc cgagcagatg tagatgaggc cgtcgtaggc gtccacgatg
     4081 gcccggtact tggcccagtg ttcctgggcg gcttcctgcg cctgccgccg ggccgtgacg
     4141 tcctcgatgt agccgtagta gtgcgtgatc gcgcccccgg cctcgcgccg caccacggtg
     4201 aagtcatgga gccagtgggc gcggccgccc ttgcccatga tccggtactc ctgctcgaag
     4261 tgcgtcgcgc cggcctcgct gaattgccgg acctcctccg ccacgcgggc aaggtcgtcg
     4321 gggtggacga tcgaggcata ggggagccgg ccgctgacaa aatcatcggg ccggaagcca
     4381 aactgctcga cgttgggcga cacatactcc acgggccacc cctcggcggc cttccacttg
     4441 aacatgaccg tgggcccgct ctcgaacagg cggagggcct cgtcgggact tagcgatgcg
     4501 gcatccatga acgtcctaaa aaatccggcc gtttatgaag attgcgcgcc gggatgtcaa
     4561 gcccggcgcg gcggccgcga aacccagggt tgcttttcaa ggaaccggcg tgtagaaaca
     4621 aaaaactaca tcatgaccgg gaatttctac cccctgccgg cgaaaggatc tcatggcgcg
     4681 aattctgacc gatgaaacct ccctgcaccg gaaacgcgat tccctgcgaa agggagcccg
     4741 ccaactgcag tcgctggtgc gctatgaact cggcggggcg gtcgtcctgc tggccgccgg
     4801 cgcgctggct tacaagtggt gggggcccgg cctgctggtg gtcggcgtgg cggcgctttt
     4861 cgtcagcgcc ggccactgga tgaaaagcaa ggaaaacaaa gatgaggagg gttcggcgag
     4921 cgccggcctc caaggtgagc agaacgtgac gcggcttctg cgggacggac tgcccaacga
     4981 cagctacatt ttcaacgaca tcacccttcg gcgcggcttg cggagcgccc agatggatca
     5041 cgtggtggtt tcgccgcggg gcatcttcgt cgtcgaaaca aagaactggc ggggcctgat
     5101 ctcgggggac gagcaggagg cccagtggac acaggtgaag cggcctggcg agaaaccgat
     5161 gcggcgggcc aatcccatcc tgcagaaccg gcgccagatc aacgtgatgc aggccgtcct
     5221 gaaggccggc gggatagact ggccggatat cttcccggtg ctgatctcca attcgaggaa
     5281 cgcgcagttc gagatccata atcgcagcgt gcccatcctg acgcgcttcg acgcggtgga
     5341 gtacatggtc gggcaggcgc ccagccggca atacaccgag gcggaggtga ccgcagtgat
     5401 tgagctgctg atgaagaagg cgaaataaat ccgcgtaaac ggatggataa ccgaaggaag
     5461 gaaaccggac atggcccaaa ccgccaaatt tgatgaattc cgcggattgt cggagcagcc
     5521 ctcaagcccc ccgccgaaaa aacactgggg catgctggtc ctttccctgg tcggcgtggc
     5581 cctcgtctgg tacctgctgt tctggagccg cgcgcccgag accgaggtca tcgaggccgt
     5641 ggtcgtgggc gaggaggccg agcgcgtgcc gatcaaccat gccgaggtga cggatgcgtc
     5701 gaccgaccgc ggcgccgtgg cccgcgaggg ctaccgttac aaggtccgca tcgaggacga
     5761 atcctccgac ggcgtggcgg gcgtcacgca tatcggcggg ctggtgacgt tcgtcccgga
     5821 cgcgcgcccc ggggagatgg tgatcatcga gatcacccgg ctcaaggagc ggacggcgga
     5881 tgggatggtc gtcaagcggc tgcccgagga aaccattgca tggacgcccc cgccgccccg
     5941 gccgacgcgc ccccccatgt ttacgccgga cggggtgtac acgagcgtag tgctggaact
     6001 cgggaagaag ggcgacgcga tcacgaagat caacggcaag gtggtgttcc tgccgggggc
     6061 gatgccgggc gaccgggtga cgttccgggt ggtggaggag cgcgactcgg cggcgacggg
     6121 cgaggtgctg acgcgcgagc cggggcccgt gccggcgccg gaagccttcg agaggccgcg
     6181 gccggcgatg acgcggccgc cgatgtttac gccggatggg gtatacacga gcgtggtgtt
     6241 ggagatcggg aagaagggcg acgcgatcac gaagatcaac ggcaaggtgg tgttcctgcc
     6301 gggggcgatg ccgggcgacc gggtgacgtt ccgggtggtg gaggagcgcg actcggcggc
     6361 gacgggcgag gtgctgacgc gcgaaccggg gccggttccg gcgccggaag cctttaacag
     6421 gccgcagccg gccatgaccc ggccgccgat gttccagccg gacgccgtgt ataccggcaa
     6481 ggtgacggac attggaaaga agggcgatgc gttgctcaag gtggacggga aagtcgtatt
     6541 cctgcccggg gcgcaactgg gcgacgaggt ggtattccgc gtggtttcgg agcgggactc
     6601 gacggcgcag ggcgaggtga tttcgcgtac gccggccgcg ccctgatccc aaacgagccg
     6661 ggtctcatgg cgcgcattct caaatcggag acctccctgt cgtcccgccg agtgaggctt
     6721 gcgcgggaaa tggggatgct gcgctgggtc cgctggctgg aactggccgg cgccctcgtg
     6781 ctgctggccg gcgggatcgg gctgtgggtc tggcgggaca ttcactggct ggccctggtc
     6841 ggcgccggcc tgctgttcgc atggttgggc caccatttca agatgcagga gaacagcctc
     6901 gacgcgtgga ccattggcgt cggcctggag ggagagcagg gcgtcaccga ccgcctggcc
     6961 accggcctgg atgaccgcta cctggtgctg aacgacgtgt tcatccagtt cgggcggcgg
     7021 cgggcgcaga tcgaccacgt ggtggcgggg ccgaacggca ttttcgtgat cgagacaaaa
     7081 aactgggccg ggcgaatcgt gggagacgag acgcaaaacc actgggtctt ttatgccgcg
     7141 gggaaggacg ggcaggaact gttcaatccg atcctccaaa gccagcgcca ggccaagttc
     7201 gtcgcccggc ggttgcggga ggcggggatg gactggcccg acatgcgggc gttagtcgtg
     7261 atgttttcgg aggattcaag gtgggccatc cggaaccaga cggcgccgat cctgcggccg
     7321 gccgaattgg cgccgttcat ccggaatgcg gcctccggcc ggagctaccg gccggaggag
     7381 atcgacgcgg cgggccggtg gatcatgcag gactcgggat gacgccgatg aagcgactcg
     7441 tggccaccat caatacgctg ggactgctgg gctacctgta ctgggtgggc ttcaccccgc
     7501 acaaaaagat tctctacagc cgggatggaa tcatctatgt tgtcccattc ctgctgttct
     7561 tttttgtgta cttgtcgctg
//
LOCUS       DAOOTK010000138         7525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6115_length_7525_cov_4.947523, whole genome shotgun sequence.
ACCESSION   DAOOTK010000138 DAOOTK010000000
VERSION     DAOOTK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6115_length_7525_cov_4.947523"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PK634_12380"
     CDS             <1..1319
                     /locus_tag="PK634_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOW98575.1"
                     /translation="SWLWPFSVFGWPEKGADLSFYYPTVTLSTASEIIFFWVARMIMA
                     GFEFMGDIPFREVYIHGTVRDESGLKMSKSLGNSIDPLAITEQFSADALRFSLIMLTA
                     TGQDVFLSNSKFEIGRNFGTKLWNAARFIQMQAKGAAASDAPAFDAATLSADDRHILA
                     KLHAAIAACNDNLARYRLNDYAKTMYEFLWHQFCDWYVEYAKQILYGADEKRKADVLA
                     VMHYVFSRALRLLHPLMPFLTEELWHGMGYAALHGDSYIMTEKWPEPRPEEELQGWGI
                     TPELVSYVDARHDLVRIGRTLRADYGIPPGAKVKFTVRPVSPVVGGQLESDREAIVSL
                     LKAETLTLDPAFTPVGAMPGGLSPLGTIFLSLEGLVDPAKEVEKLNGQIAETEDGLQK
                     LAKKLDNLDFVTRAPATVIEQHKARKAELVEKLEKLRKLRDTLKPA"
     gene            complement(1350..2708)
                     /locus_tag="PK634_12385"
     CDS             complement(1350..2708)
                     /locus_tag="PK634_12385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOW98576.1"
                     /translation="MARKQRIAILGTGYVGLVAAAVFADRGFRVLTSSQDAEKVNTIN
                     AGKSPFFETGLDPVIARVVKKGILRAVHGREEAVRESDICFIAVGTPSLMSGEADLSL
                     VKETAAAIGKALKKRKAYSLIVARSTIVPGTTRNIIIPIVEKHSGKKAGRDFGVCMSP
                     EFLRQGAALKDTSEPDSVVVGEFDKRSGDVLQKFANELYRGQKVPVLRMNLESAEMVK
                     YGRNTFLAMNISYINEMARIAETIPGIDITEVVKGVGADWRINPVFLNAGAGYGGSCF
                     PKDVKALISFAQLREVEPALLETVEEVNLQQASHVVGLLRKELGELKGRKIALLGLAF
                     KPGTDDMREAPAVKVANHLFAEHAQIVAYDPVAIPNAKTKFIRDTIRIRYAESAKECV
                     KGADACVILTEWDEFRKLKAGFFNKNMKKSVIIDARRIYDASFRSKVATYRGIGLGPA
                     TG"
     gene            complement(3084..3761)
                     /locus_tag="PK634_12390"
     CDS             complement(3084..3761)
                     /locus_tag="PK634_12390"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98577.1"
                     /translation="MASRPSAGPNGSVDRVGGWFTNNEPVSVEALPDAYYHFTNWTGD
                     VEAGAEQDNPLNLVMDGPKAVMAHFAASWTTNSPTPHWWLGQFGITDHFEVAVFADPD
                     GDTIPTGDEFTMNTDPTNGLSFLSVAHIGNAYGTNCYESVWTNSEPPYEEVTQTVCDV
                     VGLLLSWPADTGRVYDVEFDTDHPWGNWVPVEGLTNLTTDTGWISITNVTDAEALKLY
                     RLRVHQP"
     gene            complement(4375..>7525)
                     /locus_tag="PK634_12395"
     CDS             complement(4375..>7525)
                     /locus_tag="PK634_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98578.1"
                     /translation="LVTITDPAPEEGDYFGRSVASLGADKIIVGADQDNAGAYAAGSV
                     YLYDMSGVLLLTITNPAPNSSDYFGFSVASLDSDKIIVGAYQDDAGAANAGSAYLFDT
                     NGILLATIANPAPTKNDYFGYSVAGLGSDKIIVGAYQDDAGAADAGSAYLFDTNGILL
                     ANIANPLPAATDCFGPSVAGLGSDKILVGAVLDDAGAANAGSVYLFEPDAGGAFVRNT
                     AALTASDPADTIPANNTGTVAFAVGMEADLAIAKSGASAQLPLLATNTYTITVTNRGP
                     ATATALRIADLLPGDLQFVSATPDAGWYETASHLWNINRLGAGDSASLTLQARVQESW
                     PLAVTITNPAPASQDAFGCSVASVGSDKFIVGAFQDDAGSNNAGSAYLYDTSGALLAT
                     ITNPAPAAGDYFGNSVAGLGTDKIIVGAYSDDAGTNNAGSAYLYDTSGVLLTTITNPA
                     PAANDYFGYSVAGLGTDKILVGANQDDAGAVNAGSVYLYNTNGVLLVTITNPAPAAND
                     YFGCSVAGLGSDKIIVGAYGNSNGAIAAGSVYLFDANGVLLTTITNPAPAASDYFGIS
                     VAGLGSDKIIVGANQDDAVATDGGSAYLFDTNGVLLATITNPAPMVQAFFGYSVAGVR
                     SDRIIVGAPRDSSSGAIYAGSAYLFDTNGVFLPTIAKPALPEQALFGWSVAGVGSNKV
                     IVGAAYNSVGTTYDGSAYLYNCRTPPYSVTNRVTPLTYAQALTNEANASASASLLVGE
                     GGGTTYDLTIESPYGSTVPAVGVHEVNEGQEVTVQAISPDTRGTTQYVCTGWTATANL
                     SPASGSGTQAVVTVNGDGTLTWLWRTDFWLDPSAGPNGSVDRVGGWFTNNEPVSVEAL
                     PDACYHFTNWTGDVEAGSEQDNPLNLVMDGPRAVKAYFTAITTTNGTPLWWLAQYGIT
                     NNLEEAVFDDPDHDGIPTGDEYIANTDPTNAMSFLQTAAYGLIYGTNCYDVVTTNSEP
                     PYEVVTNVICDVIGQVMGWPCATDRVYDVQHAMALPPGIWAPMPGMTNLVPTSGMLVL
                     TNTITGDPIQFYRLRARLP"
BASE COUNT         1319 a         2453 c         2398 g         1355 t
ORIGIN      
        1 cctcgtggct ctggcccttc agcgtgttcg gctggccgga gaagggcgcc gacctctcgt
       61 tctactaccc aaccgtgacg ctttcgacgg cctcggagat catcttcttc tgggtcgcgc
      121 gcatgatcat ggccggcttc gagttcatgg gcgatatccc gttccgcgag gtctacatcc
      181 acggcacggt ccgcgacgag tcggggctca agatgagcaa gagcctcggc aattccatcg
      241 atccgctggc gatcaccgag cagttcagcg ccgacgcgct ccgcttcagt ctgatcatgc
      301 tcacggcgac cgggcaggac gtgttcctct ccaacagcaa gttcgagatc gggcgcaact
      361 tcgggaccaa gctctggaac gccgcgcgat tcatccagat gcaggccaag ggcgcggccg
      421 cctcggacgc cccggcgttc gacgccgcga cgctctccgc ggacgatcgc cacatcctgg
      481 ccaagctcca cgcagctatt gccgcctgca acgacaacct cgcgcgctac cgcctgaacg
      541 actatgccaa gacgatgtac gagttcctct ggcaccagtt ctgcgactgg tacgtcgagt
      601 acgccaagca gatcctctac ggcgcggacg aaaagcggaa ggccgacgtg ctggcggtca
      661 tgcactacgt cttctcccgc gcgctgcgcc tgctgcaccc gctgatgccg ttcctgaccg
      721 aggagctgtg gcacgggatg ggctacgcgg cgctccacgg cgattcgtat atcatgaccg
      781 aaaaatggcc cgagccgcgc cccgaagagg agttgcaggg gtgggggatt acgccggagc
      841 ttgtaagcta cgtggacgcc cggcacgacc tcgtgcgcat cgggcggacc ctgcgcgcgg
      901 actacggcat cccgccgggc gcgaaggtga agttcaccgt ccggccggtc agcccagtcg
      961 tgggggggca gttggagtcc gaccgcgagg cgatcgtttc gctgttgaag gcggaaacgc
     1021 tgacgctgga cccggccttc acgccggttg gggccatgcc gggcggcttg agcccgctgg
     1081 gcacgatctt cctgtcgctc gagggcctcg tggacccggc gaaggaagtc gagaagctca
     1141 acggtcagat cgcggagacg gaggatggcc tccagaaact ggccaagaaa ctggacaacc
     1201 tggacttcgt cacccgcgcg cccgccaccg tgatcgagca gcacaaggcc cgcaaggcgg
     1261 aactggtcga gaagctcgaa aagctgcgca agctgcggga cacgctgaag cccgcctgac
     1321 acagccgcgg cgcggacagc ccgcggggct catcccgtgg ccgggcccaa gccgatgccg
     1381 cgataggtcg cgaccttcga ccggaaagac gcgtcgtaga tgcgccgcgc gtcgatgatc
     1441 acggacttct tcatgttctt gttgaagaaa ccggctttca gcttcctgaa ctcgtcccac
     1501 tcggtcagga tcacgcaggc atcggcgccc ttcacgcact ccttcgcgct ttccgcatag
     1561 cgaatccgga tggtgtccct gatgaatttg gtctttgcgt tcgggatggc gacgggatcg
     1621 taggcgacga tctgcgcgtg ctccgcgaag aggtggttgg ctaccttgac ggcgggggcc
     1681 tcgcgcatgt cgtccgtgcc gggcttgaac gcgagcccga gcagggcaat cttcctgccc
     1741 ttcagttcgc ccagttcctt gcggagcaag cccacgacgt gcgaggcctg ctgcaggttc
     1801 acctcctcga ccgtctcgag cagggcgggt tcaacttccc gcagttgcgc gaaggagatg
     1861 agggccttca cgtccttggg gaagcacgat ccgccgtagc ccgcgccggc gttgaggaag
     1921 accgggttga tgcgccagtc ggcgcccacg cccttgacca cctccgtgat gtcgatgccc
     1981 ggaatggtct cggcgatacg cgccatctcg ttgatgtacg agatgttcat ggccaggaac
     2041 gtgttccggc cgtatttcac catctccgcg ctctcgaggt tcatgcgcag gaccggcacc
     2101 ttctgccccc ggtagagctc gttggcgaac ttctggagga cgtcgccgga gcgcttgtcg
     2161 aactcgccga ccacgacgct gtccggctcc gaagtgtcct tcaaggccgc cccctggcgc
     2221 aggaattccg ggctcatgca gaccccgaaa tcccggcccg ccttcttgcc ggagtgcttc
     2281 tccacgatcg ggatgatgat gttccgggtc gtgccgggga caatggtgct gcgggcgacg
     2341 atcagcgaat acgccttccg cttcttgagg gccttcccga tggccgcggc ggtctccttc
     2401 acgaggctca agtccgcctc gccgctcatc aggctcgggg tccccacggc gatgaagcag
     2461 atgtccgatt cccgcacggc ctcttcccgg ccatgcacgg cccggaggat gcccttcttc
     2521 accacccgcg cgatcacggg atcgaggccg gtttcgaaga aaggcgactt cccggcgttg
     2581 atggtattga ccttttccgc gtcctggctg gaggtgagga cccggaaacc ccggtccgcg
     2641 aagaccgcgg ccgcgaccag ccccacgtaa ccggtcccga gaatggcaat gcgctgtttc
     2701 ctggccatgg cgatgctcct tctgggttga aagcggcggg cactatatcc gtccgcccgc
     2761 ggggaagtca atccgcgccc cgccgaaaaa tccgcggcag gcaaagaaga ccgcggatac
     2821 acgcggatgg aaaccccgcc tatcccggtt tattcctgaa cgccagccgt tcatgaataa
     2881 accgcttggc gcacgggctg cggtgcagtc cgtgcgccaa gacgtgacga gacgcggtgt
     2941 gcgccgcgtc ccgaacgtgg taagccccgt ttcggtccgc cgtggcgggc cgatcatcgc
     3001 gtgaccgcgt tgaggcgcgg tacgcgactc tcacgcggcg gccgcgaagc ggcggattat
     3061 tcgcgcttcg cgaataatcc gggctatggc tggtggaccc gcagccggta cagcttcagc
     3121 gcctcggcgt ccgtcacgtt ggtgatggag atccagccgg tgtcggtggt caggttcgtc
     3181 agcccctcca ccgggaccca gttcccccac gggtggtccg tgtcgaattc cacgtcgtag
     3241 acacggcccg tgtccgccgg ccagctcaac agaagcccca cgacatcgca cacggtctgc
     3301 gtgacctctt cgtacggcgg ctcgctattc gtccatacgc tctcgtagca gttggtcccg
     3361 tacgcgttcc cgatatgagc cacgctcaag aaggacaggc cgttcgtcgg gtccgtgttc
     3421 atcgtgaact cgtcgccggt cgggatggtg tccccgtcgg gatcggcgaa caccgccact
     3481 tcgaagtggt cggtgatgcc gaactgtccg agccaccagt gcggcgtcgg actgttcgtc
     3541 gtccacgagg ccgcgaagtg cgccatgacc gccttgggcc cgtccatgac caggttcagc
     3601 ggattgtcct gctccgcgcc ggcctccacg tcgcccgtcc agttggtgaa gtgatagtaa
     3661 gcgtccggca gcgcttccac ggacaccggc tcgttgttgg tgaaccagcc gccgacccgg
     3721 tccaccgagc cgttcggccc ggcgctcggc cgggaggcca acacaaaaag cggggaaaat
     3781 ggggggggtg catgggcatg aaacactttc tgtgtccctg aaaaatttgc ggcttcctgc
     3841 aactggaaac agcgaggaac ccagaaacaa cccatcgccc ggctctgaga atccggacaa
     3901 caggcttcgt cgccttaaat gcgccctatt tccgccggcg acgcaggaag ttgccgccca
     3961 agccgacggt cccccagcac gagcagcgcg caggtcgagg gctcagggat ggcggcggtt
     4021 accccattaa aggctggggg gctagtcaac gcgaagaagt ggcatttttc ccgggggagc
     4081 ggcagttgta gctccggcgc tctgccgccg gagattcttt ccgccgacgg agcggcggaa
     4141 ctgcagcatg cgggccttgg aaattggatt cggacatcgc cttcacctgc ccaagcccat
     4201 cctgaacaga aggaaacaaa gcgaacaaag acctgatggc gccatttcct ctgttacctc
     4261 ctgttcatat atcctggctt tgaatagctg accctgccga atgccaaggc ccaccggcat
     4321 gggcggcgcg cccccggagg ccgggcctcc gggggtgggc catccgcggc cgctctacgg
     4381 cagccgcgcg cgcaggcgat agaactggat gggatcgccc gtgatcgtgt tggtgagcac
     4441 cagcatgccg gacgtcggca ccaggttcgt catgcccggc atcggcgccc agatcccggg
     4501 cggcagcgcc atggcatgct gcacgtcata gacgcggtcc gtggcgcagg gccagcccat
     4561 cacctggccg atcacatcgc aaatgacgtt tgtgaccacc tcgtagggcg gctcgctgtt
     4621 ggtcgtcacc acgtcatagc agtttgtgcc atagatcagc ccataggccg ccgtttgcag
     4681 gaacgacatt gcgttggtcg ggtcagtgtt ggcgatatac tcatcccccg tcgggatgcc
     4741 gtcgtggtcc ggatcgtcga acaccgcctc ttccaggttg ttggtgatgc catattgcgc
     4801 cagccaccag agcggggtgc cattagtggt cgtgatcgcc gtgaagtacg ccttcaccgc
     4861 cctgggcccg tccatgacca ggttcagcgg attgtcctgc tccgagccgg cctccacgtc
     4921 gccggtccag ttggtgaagt gatagcaagc gtccggcagc gcttccacgg acaccggctc
     4981 gttgttggtg aaccagccgc cgacccggtc caccgagccg ttcggcccgg cgctcgggtc
     5041 cagccagaag tccgtacgcc agagccacgt cagggtgccg tccccgttga cggtcacgac
     5101 ggcctgggtg cccgagccgc tggccgggga caggttcgcc gtcgccgtcc agccggtgca
     5161 gacatactgc gtcgtgccgc gggtatcggg cgaaatggcc tggaccgtca cctcttgccc
     5221 ctcgttgacc tcgtgcacgc ccaccgccgg gacggtcgag ccgtagggcg attcgatggt
     5281 caggtcatag gtcgtcccgc cgccctcgcc cacgaggagg gacgcggagg cggaggcgtt
     5341 ggcctcgttg gtgagggcct gggcgtaggt cagcggagtc acccggttgg tgacgctata
     5401 aggcggcgtc ctgcaattgt aaagataagc gctcccatca taggtcgttc cgacagagtt
     5461 gtaagcagcg cccacgatga ctttattcga tcccacgccc gccaccgacc agccaaagag
     5521 agcttgctcc ggcagcgcgg gtttggcgat cgtcggcaga aagaccccgt tcgtatcgaa
     5581 gagataggca ctcccagcat agatagctcc ggaggagctg tctcggggag cgcccacgat
     5641 gattctgtcc gatctcacgc ccgccacgga atagccgaag aaagcttgca ccatcggcgc
     5701 gggattggtg atcgtcgcca gcaagacccc gttcgtgtcg aagagatagg cgctcccgcc
     5761 atcggtcgct acggcgtcgt cctgattggc gcccacaatg atcttatccg agcccaagcc
     5821 cgccacggaa atgccgaagt aatcgctcgc cgccggcgcg gggttggtga tggtcgtcaa
     5881 caagacccca ttggcgtcaa agagatacac gctcccggca gcgatcgctc cgttggagtt
     5941 cccataagcg cccacgatga tcttgtccga gcccaagccc gccacggaac agccgaagta
     6001 atcgttcgcc gccggcgcgg gattggtgat cgtcaccagc aagaccccgt tcgtgttgta
     6061 gagatagacg ctcccggcat tgaccgctcc ggcgtcgtcc tgattggcac ccacgaggat
     6121 cttgtccgtg cccaggcccg ccacggaata gccgaagtaa tcgttcgccg ccggcgcggg
     6181 gttggtgatg gtcgtcagca agaccccgct ggtgtcgtag agataggcgc tcccggcatt
     6241 gttagttccg gcgtcgtcag aataggcgcc cacgatgatc ttgtccgtgc ccaggcccgc
     6301 cacggaattg ccgaagtaat cgcccgcggc cggcgcaggg ttggtgatgg tcgccagcaa
     6361 ggccccgctg gtgtcgtaga gataggcact cccggcattg ttacttccgg cgtcgtcctg
     6421 aaaggcgccc acgatgaact tgtccgagcc cacgctcgcc accgaacagc cgaaggcatc
     6481 ttgcgaagcc ggcgcggggt tggtgatcgt caccgcgagg ggccaggact cctgcaccct
     6541 ggcctgcagg gtcagcgacg cgctgtcgcc ggcacccagc cggttgatgt tccaaaggtg
     6601 gctggccgtt tcgtaccagc ccgcatccgg cgtggccgaa acgaactgca aatcgccagg
     6661 taagaggtcg gcgattcgga gagccgtggc cgtggccggg ccgcgattgg tcaccgtgat
     6721 cgtgtaggtg ttggtcgcca gcaacgggag ctgggcagag gcgccggact tggcgatcgc
     6781 gaggtctgcc tccatcccca ccgcaaaagc caccgtgccg gtattgttgg cgggaatcgt
     6841 atccgccgga tcggacgcgg tcaacgccgc cgtgttgcgg acaaacgcgc cgccggcgtc
     6901 tggttcgaaa agatacacgc ttccggcatt ggccgctccg gcgtcgtcca aaacggcacc
     6961 cacgagtatc ttgtccgagc ccaggcccgc cacggatggg ccgaagcaat cggtcgcggc
     7021 cggcaggggg ttggcgatgt ttgccagcaa gatcccgttc gtgtcgaaga gataggcact
     7081 cccggcatcg gccgcaccgg cgtcgtcctg ataggcgccc acgatgattt tgtcagagcc
     7141 caggcccgcc acggaatagc cgaagtaatc gttctttgtc ggcgcggggt tggcgatggt
     7201 cgccagcaat atcccgttcg tgtcgaagag ataggcgctc ccggcattgg ccgctccggc
     7261 gtcgtcctga taggcgccca cgatgatctt gtccgagtcc aggctcgcca ccgaaaagcc
     7321 gaagtaatcg ctcgagttcg gcgcgggatt ggtgatcgtc agcagcaaga ctccactcat
     7381 gtcgtagaga tatacgctcc cggcagcata cgctccggcg ttgtcctgat cggcgcccac
     7441 gatgatcttg tccgcgccca ggctcgccac cgaacggccg aagtaatcgc cctcttccgg
     7501 cgcggggtcg gtgatcgtca ccagc
//
LOCUS       DAOOTK010000139         7445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6199_length_7445_cov_4.978078, whole genome shotgun sequence.
ACCESSION   DAOOTK010000139 DAOOTK010000000
VERSION     DAOOTK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6199_length_7445_cov_4.978078"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PK634_12400"
     CDS             <1..646
                     /locus_tag="PK634_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98579.1"
                     /translation="VVPIDPTYAQECPLGQVALEWAAHQSEDQRKALEQLVALSRTLN
                     SAEGLCDALRKLTERSLPDQVAVALALKAKAYTGPSVAAAVWDVLSDTEWRQRVLGGV
                     EDRVLGLLIEGFSLLQVDAQEKWFSHLPHFVADLCEKTEDKKRCHQLFLYTIHTSLAS
                     DTVSAVRRLLNGDKKALMAEFAADYRARVEAMWPFYPAWVQGRLRALIASLRVI"
     gene            741..1145
                     /locus_tag="PK634_12405"
     CDS             741..1145
                     /locus_tag="PK634_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98580.1"
                     /translation="MLPDKSLREVELTQTEWLLLHAKATTRENAGIRRLNKHPLNQAA
                     LERLTAFGGGPMQAGRLASMHTLTLAQHVLAAEEVPTWKTPWGITADYSRNFASMRKA
                     LSILAQQLTPEEIAGASPEEAGHLILEALCAE"
     gene            1602..3197
                     /locus_tag="PK634_12410"
     CDS             1602..3197
                     /locus_tag="PK634_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98581.1"
                     /translation="MDKHRRVAPRRLRVESLAPPFSCFTQVEESFGGNPAVHRCFSLA
                     RSFKALTLVTEEIDAEGLLADENAEIAGLYNTFQPGPIVRISFWLDTVQPKQDSADLP
                     ADRLVGYAILKKDILSSTKLDHEGWHVFEAVFRKYDHRHNCVPAQCSYSLRVGATELQ
                     LPGVLYAQQNGLNKACAHVALRSLLSRFSPGNDLRNSDINKIARALGPSTTPPEKGLS
                     AQQIQAVLKHYGVNYRDVDYEVAQKKNPTVRKTQPYQKYLYSAIESGCGGLLGFSMSG
                     PKADPADPDKHIIPFFGHTFNKDTWAPDADQAYFNIGGGIGFIPSENWTSSFIGHDDN
                     FGPNFCIPRLYVNPGQVDYVAEILPQGTAYSGMIAEALALQFIYSLHPYLIKSNAWSR
                     RLSELAHPSMQRVILRAICVSSSTYVEHLGTISDWDGCRETPALVSELSNLLPKRVWV
                     VEISIPPLFAAEERKVGEILLDATKAPKQKGKMAHKIDFGLFLLARLPTQYFVLKAET
                     ATGPVFSRIQSALESHVPVLEIQ"
     gene            complement(3502..3576)
                     /locus_tag="PK634_12415"
     tRNA            complement(3502..3576)
                     /locus_tag="PK634_12415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3542..3544),aa:Gly,seq:ccc)
     gene            3670..4212
                     /locus_tag="PK634_12420"
     CDS             3670..4212
                     /locus_tag="PK634_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98582.1"
                     /translation="MPYLPSMDILAHAVYGATICSRPGLAGGRTGAGRPWFRDWTAWA
                     AMLFGILPDALSMGLPFLQFEHGDHVGHFFREFDGAGLIHYRYAHSLLVALAVSGLLR
                     LLRRPLFLPSLAWPLHLALDALTHASPGKFHTELFYPLSSWAFNGLSWWQHPGLVALY
                     WLALPAAWLALCAWRARGRP"
     gene            4268..6712
                     /locus_tag="PK634_12425"
     CDS             4268..6712
                     /locus_tag="PK634_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOW98583.1"
                     /translation="MKLVVIGGVAAGMSTAARARRLDENAEIVVLERSGYVSFANCGL
                     PYHIGGVIRDRAQLLLQTPESLKASLDLDVRTGHEALAIDRAARKVRVREVSSGREYD
                     EPYDQLALCPGATPIKPPLPGIDHPRVFVLRNMDDMDAIQGAIERGAKSAVVIGGGYI
                     GVEMAENLRHRGLAVDLVEMLDQVMPPLDKEMVAPLEDHLRRHGVNLHLGTAAAAFRD
                     TGGRVAVELKNGAVLTADLVVLSAGVRPDTALAKAAGLELGPRGGIKVDAHLRTSDPS
                     IYAAGDAVETPHTVLPDSWLIPLAGPANRQGRIAAENICGRSTTYASTQGTAIVKIFD
                     MTAGGTGAIEKSLVRAGAPFRKVYLHPSGHAGYYPGTAPMHIKVLFAPDTGRLLGAQV
                     VGFDGVDKRLDVFATAIRAGLTVYDLENLELSYAPPFGSAKDPVNMAGFIGANLLRGD
                     IEFWYAEDYPARTEGAVILDVRGAPEYETWHIPGAINIPLGQLRSRLGELDPGRPARV
                     YCAVGFRSYLAYRLLKQRGFRDVANLSGGSKTFRQWHRQLGAEEAEVPFLSYAEEKMA
                     EQSAPTGKVVELDCTALQCPGPILRLNEKMDGLAAGDEVLVRVADPGFAADAPAWCGR
                     HGHELVALEQKGALLEARIRKGGGAARPPERADRSGQKTFVVFSGDLDRVMAAFIIAN
                     GALAMGSQVSLFFTFWGLNALRKSPAPPVRKGWTEKMFGAMMPRGAEALKLSRMNMLG
                     AGTAMMKRVMKDKHVTGLPELIETARKGGARLIACTMTMDVMGLKREELIDGVELGGV
                     ATFLGEADRAGTTLFI"
     gene            6830..>7445
                     /locus_tag="PK634_12430"
     CDS             6830..>7445
                     /locus_tag="PK634_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98584.1"
                     /translation="MGKALKPLVIVLLLLSIASLVLGILLFSKREILKERTRLHETGL
                     ADIAAKFHYDQLNVAALKDVTQLKPQLDLMAVAAGNQYDDLQNTKADLAATKTKLAET
                     EQMLADTRAELEVAKNQIVQLNATIEQKDAEIAQANGKIDQLEQDKIGLQTQIDEINL
                     QMAKKEDQIADLKSQITTLENYIAKIEPPTAGGKKLIGLTGHIMV"
BASE COUNT         1496 a         2369 c         2262 g         1318 t
ORIGIN      
        1 cgtcgtgccc atcgatccca cctatgcgca ggaatgtccg ctcgggcaag tggctcttga
       61 atgggccgcc caccagtcgg aggatcagag gaaagcgctt gagcaactcg ttgcgttgag
      121 tcgaacactg aactccgcgg agggactctg cgatgcactc cgcaagttga ccgagcgctc
      181 tttgcctgat caggtcgctg tagcccttgc cctaaaagcc aaggcttaca ctggcccatc
      241 ggttgcggcg gcagtctggg acgtcttgtc ggacacagaa tggcggcaac gcgtactcgg
      301 gggcgtcgag gatcgcgtcc ttgggttact gatcgagggc ttcagccttc ttcaagtcga
      361 tgcccaggag aaatggttct cgcacctgcc ccactttgtc gctgacttgt gcgagaagac
      421 cgaggacaag aaacgctgcc atcaactatt cctctacacc atccacacct cgctcgcttc
      481 ggacaccgtc agcgccgtgc gtcggttgct gaatggtgac aagaaggcgc tgatggccga
      541 gtttgccgcc gactaccgcg ctcgtgtcga ggccatgtgg ccattttatc cggcatgggt
      601 tcaaggccgt ctgcgcgcat taatcgcaag cctgcgagtc atataaaggc gtttgggaac
      661 acttcatgaa tgcccgccag cttaagggcc gcttaatagc atggccggca cccaaccggc
      721 cggggccttg ggcgtaccga atgctgcctg acaagtcgct gcgtgaagtc gaactgacgc
      781 agacggaatg gctcctgttg catgccaagg cgacaaccag ggagaatgca ggcatccggc
      841 gattgaacaa gcaccccttg aaccaagccg cactggagcg ccttacggct ttcggcggag
      901 gccccatgca agctggcagg ttggcgtcca tgcacacgct gactctggcg caacatgttc
      961 tcgccgccga agaggtcccc acatggaaaa caccgtgggg catcaccgct gactactcca
     1021 gaaacttcgc gtccatgcgg aaggcgcttt cgatcctcgc tcaacagttg acccccgaag
     1081 agatcgccgg agcctccccc gaagaggcag gccatctgat tcttgaagct ctctgcgcag
     1141 agtgaaatga cctgaaacaa gcgcatgagc agatgcaccc ccagcgagga cgatggctgg
     1201 ccgagaacct ggagaagtgc accgcggctt cggtccccga gtcaagcgcc tgaagcctga
     1261 cgaagggccg gaagtggtcc agaagcgacc tgaacagctt ctcgcagagg atgtcttaaa
     1321 cagcggaagt aatctttcaa ataaaggttg cgcgcgacca ggcaatagac tagtttgaag
     1381 cgcataaacg caagcggggg aggccatggc acagacctat tcaagcccca aaattgcacg
     1441 ggcactgaat aagcactttg ggaatcgaac caccaccgtc cgggttacga tgaaacacgc
     1501 gcaaactgtg ggccgcttcg ttcagaaggt tgaagcggcg cagcagagcg cggcaaacag
     1561 caaattggtg ttcaggtagg cttgactgag accggcgcgt catggataag catcgccgcg
     1621 tcgcaccccg caggttgcga gtcgaatcgt tggcgccacc tttttcctgc ttcacccaag
     1681 tggaagaatc gtttggcggc aatcctgccg ttcacaggtg tttttctctt gcgcgatcat
     1741 tcaaggcgct gacactcgtc accgaagaga tcgacgcaga aggcttgctc gcagacgaga
     1801 acgcggagat cgctggcctt tacaacacgt tccagccagg ccccattgta cgcatttctt
     1861 tctggctcga caccgtacag cctaagcaag actcggccga tctgccggct gatcgtttgg
     1921 ttggttatgc cattctgaaa aaggatattc tttcatctac caagctcgac catgaaggat
     1981 ggcacgtctt tgaagctgtc tttcgcaagt acgatcatcg tcacaactgc gtgcccgctc
     2041 aatgcagcta ctcccttcgt gtcggcgcaa ccgaactgca attgccaggc gtcctgtatg
     2101 cgcaacagaa cggattaaac aaggcgtgtg ctcacgttgc gctgcgctcc ctcttgtcaa
     2161 ggttctcgcc gggaaacgac ctgcgaaact cggacatcaa caagatcgcc cgagccctcg
     2221 ggccttctac cacaccgcct gaaaaaggat tatccgcgca acaaatccaa gcagtattga
     2281 agcactacgg agtgaactac cgtgacgtcg attacgaagt agcccagaag aagaacccaa
     2341 cggtaagaaa aactcaacct taccagaagt acttgtattc tgccatagaa tctggatgcg
     2401 gagggctgtt ggggttcagc atgtcgggac cgaaggcgga ccctgcagat cctgacaagc
     2461 atattatccc tttctttgga cacacattca acaaggacac ttgggcaccc gatgccgatc
     2521 aggcatattt caatatcggc ggcgggatcg gcttcatccc gagcgaaaac tggaccagca
     2581 gctttatcgg ccatgatgac aacttcggcc caaacttctg cataccccgg ttgtacgtta
     2641 acccgggaca agttgactac gtggccgaga ttctccctca aggcactgcc tacagcggaa
     2701 tgattgccga agccctcgcc ctgcagttca tttactccct tcacccgtat ctgatcaaaa
     2761 gcaacgcgtg gtcacggcgc ctgtcggagt tggctcatcc cagcatgcaa cgcgtaatcc
     2821 tacgggccat ctgtgtatca agtagtacct acgtagagca tcttggaacg atcagtgact
     2881 gggacggctg tcgcgagact cccgccctag taagtgaatt atccaatctg ctccccaaac
     2941 gagtgtgggt ggttgagatc tcgattcccc cgctatttgc agccgaggaa cgaaaagttg
     3001 gggaaattct gctggacgcc acaaaggctc ccaaacaaaa gggcaagatg gcgcacaaga
     3061 tcgattttgg gctcttcctt ctggcacggc ttcccaccca gtactttgtt ctcaaggccg
     3121 aaacagcaac ggggcctgtt ttcagcagaa tccaaagcgc attggagtcc catgtcccgg
     3181 tgcttgaaat tcagtagcca cttctcgaaa catcgcggac caggccgtca agatcctctg
     3241 cctatctgct cggaaccggc caagaaccca accgaagcgt ccgtcatgcg cttctcgggt
     3301 ccttggccgc ccctttgttc gttctgctca acccaagccc gccacgggcc tttaaagtga
     3361 accatcgttc actgtgttga caatcgttga caatccggaa gtcttaagtg ggcatttatc
     3421 ggctgctatc cgccaagcag ggggatcatg aaaaacagcg cggattatag agaaaacgcc
     3481 tgattttatt gggggaaaag ttggagcggg agacgggagt cgaacccgcg acgttcagct
     3541 tgggaagctg acattctgcc actgaattac tcccgcccca agccgacaac attaacctaa
     3601 cagatcaggc cgcgggcgca agcgtgtttt ttttcgcgct cgcgcggacg gcatgacaga
     3661 catcgcgcca tgccgtatct tccctccatg gatatcctgg cccatgccgt gtacggcgcg
     3721 acgatctgca gccgcccggg cttggcgggc ggccggaccg gcgccggacg cccgtggttc
     3781 agggactgga ccgcctgggc ggccatgctt ttcggcattc tgccggacgc cctctcgatg
     3841 ggcctgccgt tcctccagtt cgagcacggc gaccacgtgg gccatttctt ccgcgagttc
     3901 gacggcgcgg gcctgatcca ttaccgctac gcgcacagcc tgctcgtcgc cctggcggtc
     3961 tccggcctgc tccgcctcct gcgccggccg ctcttcctgc cttcgctggc ctggccgctc
     4021 cacctggccc tggacgccct gacgcacgcg agtccgggaa agtttcacac ggaactgttc
     4081 tacccgctgt cctcgtgggc cttcaacggg ctctcctggt ggcaacaccc cgggctcgtg
     4141 gcgctctact ggctcgcgct gccggcggcg tggctagcgt tgtgcgcctg gcgcgcgcgc
     4201 gggcggcctt gagactggac ccggcccgct tttgcgttat cttctcccga cccaaaagga
     4261 gcaggatatg aaactagtgg tgatcggcgg agtcgcggcg ggcatgtcca cggcggcgcg
     4321 cgcccggagg ctggacgaga acgcggagat cgtggtgctg gaacgcagtg gctacgtgtc
     4381 cttcgccaat tgcgggctgc cctaccatat cggcggcgtc atccgcgacc gcgcccaact
     4441 cctcctccag acccccgaga gcctgaaggc ctcgctcgat ctcgatgtgc ggaccggcca
     4501 cgaggcgctg gccatcgacc gcgcggcccg gaaagtccgc gtgcgcgaag tctcctccgg
     4561 ccgggaatac gacgagccgt acgaccagct cgcgctgtgt cccggcgcga cgcccatcaa
     4621 gcccccattg cccggcatcg accatccccg cgtgttcgtg ctgcgcaaca tggacgacat
     4681 ggacgcgatc cagggcgcca tcgagcgcgg cgcgaagagc gccgtcgtca tcggcggcgg
     4741 gtacatcggc gtcgagatgg ccgagaacct gcgccatcgc ggcctcgcgg tcgacctggt
     4801 cgagatgctc gaccaggtca tgccgccgct cgacaaggag atggtggccc cgctcgagga
     4861 ccacctgcgg cgccacggcg tgaacctgca cctgggcacg gcggccgccg cgttccgcga
     4921 cacgggcggc cgggtcgcgg tcgaactcaa gaacggcgcg gtcctgacgg ccgatctcgt
     4981 cgtgctgtcc gccggcgtgc ggccggacac ggcgctggcg aaggccgcgg gcctggaact
     5041 cggaccgcgc ggcgggatca aggtggacgc ccacttgcgc acgtccgacc cgtccatcta
     5101 cgcggccggc gacgccgtcg agacgccgca taccgtcctg ccggactcgt ggctgatccc
     5161 gctggccggg ccggccaacc gccagggccg catcgcggcg gaaaacatct gcgggcggag
     5221 cacgacctac gccagcacgc agggcacggc gatcgtgaag atcttcgaca tgaccgcggg
     5281 cgggaccgga gcgatcgaga agagcctggt ccgcgccggc gcgcctttcc gcaaggtcta
     5341 ccttcatcct tccggccacg cgggctacta ccccggcacg gccccgatgc acatcaaggt
     5401 cctcttcgcg ccggacacgg gacgcctgct cggcgcccag gtcgtcgggt tcgacggcgt
     5461 ggacaagcgc ctggacgtct tcgccacggc catccgggcc gggctgacgg tctacgacct
     5521 ggagaacctg gagctttcct acgcgccgcc gttcgggtcc gccaaggacc cggtcaacat
     5581 ggccgggttc atcggggcca acctgctgcg cggcgatatc gagttctggt acgcggagga
     5641 ttaccccgcc cgcacggagg gcgccgtcat cctggacgtg cgcggcgcgc cggagtacga
     5701 gacctggcac atccccggcg cgatcaacat tccgctgggc cagctccggt cgcgcctcgg
     5761 cgagctggac cccggccgcc cggcccgcgt gtactgcgcc gtcggtttcc gcagctacct
     5821 ggcctaccgc ctgctgaagc agcggggctt ccgggatgtc gccaaccttt cgggcgggag
     5881 caagaccttc cgccaatggc accgccaact cggggcggaa gaggccgagg tgccgttttt
     5941 gtcctacgcc gaggaaaaga tggcggaaca gtccgcgccg acgggcaagg tcgtggaact
     6001 ggattgcacg gcgctgcagt gccccggtcc gatcctgcgc ctgaacgaga agatggatgg
     6061 tctcgccgcg ggcgacgagg tgctcgtgcg cgtggccgat cccgggttcg cggcggacgc
     6121 cccggcgtgg tgcggccggc acggccacga actcgtagcg cttgaacaga aaggcgccct
     6181 gctcgaggcg cggatacgca agggcggagg ggcggcccgg cccccggaaa gagcggaccg
     6241 cagcgggcag aagactttcg tcgtcttctc cggcgacctc gaccgggtca tggcggcgtt
     6301 catcatcgcc aacggcgcgc tggccatggg cagccaggtc agcctgttct tcaccttctg
     6361 gggcctgaac gccctgcgga aaagcccggc gccgcccgtt cgcaaaggat ggacggaaaa
     6421 gatgttcggc gccatgatgc cgcggggggc cgaggcgttg aaactgtcca ggatgaacat
     6481 gctgggggcc ggcacggcca tgatgaagag ggtcatgaaa gacaagcatg tcacgggcct
     6541 gccggagttg atcgaaacgg cccggaaagg cggcgcccgg ctgatcgcct gcacgatgac
     6601 catggacgtc atgggattga agcgcgagga actgatcgac ggggtcgaac tgggcggcgt
     6661 cgccacgttc ctgggcgagg cggaccgcgc ggggacgacg ctgttcatct gaccggaacc
     6721 gccgccggag gggggcgccc ggcgtcgttt ataccattga cagcgccacc gccctcttct
     6781 agagtccctt ccagatgatg agcaaccgct tatcgcggag gtgactcgta tgggcaaggc
     6841 gcttaaaccg ttggttatcg ttctcctcct cttgagcatc gcatccctcg tcctggggat
     6901 cctgctgttc tccaagcgcg agatcctcaa ggagcgcacg cggctgcacg agacgggcct
     6961 ggccgacatc gccgccaagt tccactacga ccagctcaac gtcgccgccc tgaaggacgt
     7021 cacccagctc aagccccagc tcgacctgat ggccgtcgcc gcgggcaacc agtacgacga
     7081 cctccagaac accaaggccg acctcgccgc cacgaagacc aagctcgccg agacggagca
     7141 gatgctcgcc gacacccgcg cggaactcga ggtggccaag aaccagatcg tccagctgaa
     7201 cgccacgatc gagcagaagg acgccgagat tgcgcaggcc aacgggaaga tcgaccagct
     7261 ggaacaggac aagatcggcc tccagacgca gatcgacgag atcaacctgc agatggcgaa
     7321 aaaagaagac cagatcgcgg atctgaagtc gcagatcacc acgctggaga actacatcgc
     7381 caagatcgag ccgccgacgg ccggcggcaa gaagctgatc gggctgaccg ggcacatcat
     7441 ggtgg
//
LOCUS       DAOOTK010000140         7443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6203_length_7443_cov_4.869247, whole genome shotgun sequence.
ACCESSION   DAOOTK010000140 DAOOTK010000000
VERSION     DAOOTK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6203_length_7443_cov_4.869247"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK634_12435"
     CDS             <1..234
                     /locus_tag="PK634_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98585.1"
                     /translation="HASARDFLRHIHEMGLTGGAVSRAPAPLARNELARVIQEYDRRH
                     QDGAPGVKATYAVAYVSAVGAEHPPAEPSRAAG"
     gene            complement(217..3285)
                     /locus_tag="PK634_12440"
     CDS             complement(217..3285)
                     /locus_tag="PK634_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW98586.1"
                     /translation="MKFRSSMLWLLTLPAIMLIAGATVWASRRADGLLRRELIQQARV
                     ATLAINVERVQNLQGSDADLRSPDYLRLKEQLTNMRKANDRCRFLYLMGRRADGMIFF
                     FVDSEPADSPDYSPPGQTYDEVSRFVTAVFDSEKESSEGPFTDRWGTWVSAFVPMLQP
                     ETGRLLAVFGMDIDARDWRKIVMARAALPLTLAAVAILLGLGVIGLHHANRRVRHGQE
                     QLLESRDNLQSVLTSSPVGMLVIDADERILFFNAVAEQMVRRSEGSLGALKCGDFIRC
                     VNRERDPRGCGSTDLCPACRLNLAIRSALAEDGDPAPRHGEAEVCMESHPAPRWLSFR
                     ASSVVMNKQRCAVLAIDDITEQKHASEMVREALARFSAVVESNPLVAVESYAPDGTIL
                     QWNQAATRLFGATQEEAVGNRMQDLLQEDPETGGQNTIAEIWRTGQGSPPAERRVTAK
                     NGEERWLYSVMFPVFNKGRVSEVFCMDVDITDRKRAEEKAVQLAAESDRARIVLLSIL
                     EDERRGQAERARLASAIEQAGEAVVITDAQGNIQYVNPAFTRVSGYAREEVIGKNPRI
                     LKGGRKGEDFYRQLWDTISSGAIWTGRLVNRRKDGVLYTEDVSISPVHDAHGKIVNYV
                     AVKRDVSRELDIEKQLAQAQKMEVIGTLAGGIAHDFNNLLQSIRGFTELALSEDEPEA
                     AQREDMLEVRKAAESAAALTRQLLAFSRRQVLEQKALDFNELIENMGKMLRRMIGEDV
                     ALNLNMEPRLWSVRADPGQMEQVILNLAVNARDAMPRGGSLTISTTNLTFAEEDLAAH
                     PDARVGRFIRISVGDTGTGMSEEIQRHVFEPFFTTKPTGKGTGMGLSSVYGIVRQHEG
                     WVHLYSELGAGTVFHIYLPATAEAVARVSAEPPLETLRGCGERILVVEDDDSVRNLMA
                     HMLGQNGYETLLAASAEEAVDIFTREKGHFALVCSDVVLPHRSGFDLVEELKKEKPAL
                     LVLMVSGYTDDRTRWPQIREKGWRYLQKPVGLRLLLRTLAEMLRPSPGGA"
     gene            complement(3305..4240)
                     /locus_tag="PK634_12445"
     CDS             complement(3305..4240)
                     /locus_tag="PK634_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW98587.1"
                     /translation="MKTASRIRILFAEDVPADVELAQRELRRGDLDFESRRVETEEDY
                     LKALDEFRPDVVISDYSMPRFNGMKALQLALRRDPRPPLILLTGSLNEETAVQCMKAG
                     AADYVLKDNMTRLPFAVKEALQQRESREAQARAEASLRESEEKFSIAFQTSPYAITIT
                     RPEDGRFMEVNDGFCSITGYRREEVLGRSSLELGLWTRAEDRSSVVDTIRRGEVVDGV
                     EFPFRVKSGDLITGLFSARIINLRGEKFVLSSINDITDRKQAEEKLRQQLNELRRWQS
                     ATLGREGRVIELKAEVNELLKRLGEKPRYGSGGGD"
     gene            complement(4254..4700)
                     /locus_tag="PK634_12450"
     CDS             complement(4254..4700)
                     /locus_tag="PK634_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOW98588.1"
                     /translation="MNKANEVEILLVEDNPNDAELTLRALKKNNLANHIVHVADGAEA
                     LDFVFARGAFKDRDIETRPRVILLDLKLPKVNGLEVLRAVKGDEHTRTIPVVVLTSSR
                     EEQDMVESYRLGVNSYIVKPVDFDKFLESVRDLGLYWLLLNQPTNG"
     gene            complement(4746..6788)
                     /locus_tag="PK634_12455"
     CDS             complement(4746..6788)
                     /locus_tag="PK634_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOW98589.1"
                     /translation="MEERLRHLTEVLQAVRNVNQLITHEKNREALVRSACTILAETRG
                     YRSAWISLLEADGRLRPAAGSGVDASLSALRTHLEAGQVPECCRLALARSEIVVMHDT
                     KRDCVHCPMAHTDCDTAVLAGPLRHGERNYGVIVVALARVMADDAEEQSLFRELVGDI
                     GFALHALEAETERTRMVEALRVNEEQYRDMVENLSDMVFSVSGAGLLTYVSPAARFLF
                     GYEPEEVIGRSFAEFIHPEDLPAVQSYFADALAGRYHASEFRVLARDGSERWVRSTSR
                     RRREKDPASGINGILSDITELRRASEALRQNEAFTTAVLDNLPVGVAVNSVDPAVSFH
                     YMNDNFPRFYRTTREALARHDGFWDAVYEDPEFREAIRKKVLEDCASGQPERMYWPDV
                     PITRAGQETAFVTARNIPVPGKPLMISTVWDITERKKAEEDIRRLNAELEERVRLRTA
                     QLEASNKELEAFAYSVSHDLRAPLRAIDGFSRIVLEDYAGKMDTEAARLLTVVRENTR
                     QMDRLITDLLALSRATRTGVRSVPVDMATLARSVYEELLPSDEAARSEFILPNLPAVR
                     GDPTLLRQVWFNLLSNAIKYSASRDKRRIEIGARAEKEEQIYFVKDNGVGFDPRYQAK
                     LFGIFQRLHKTTEFEGSGIGLAIVQRIVQRHGGRVGAEGKVDEGATFWFALPKGRV"
     gene            6768..7346
                     /locus_tag="PK634_12460"
     CDS             6768..7346
                     /locus_tag="PK634_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98590.1"
                     /translation="MPEALLHPLPFRDIHREDEQALGHALEAQLEPARFTVGIGEEVL
                     EAARLTMSISGWINCGFQATEAGVSAYPRIGLGAVNPVRLNEEYEELMDGAGVWMEFY
                     NDKRYPETGYLATSKLEALNRFAEIGGFVEGSAVKRGVFAGINKNGLLAALIAEMGMQ
                     YQSKGEAVDRLKGLADATKRDVQIYLNRIMLG"
BASE COUNT         1271 a         2462 c         2304 g         1406 t
ORIGIN      
        1 catgcctcgg cccgggattt cctgcggcac atccacgaga tggggcttac gggcggcgcg
       61 gtctcccgcg ccccggcgcc actggcgcgg aacgaactcg cgcgggtgat ccaggagtat
      121 gatcgccgac accaggacgg ggcccccggg gtgaaggcga cctacgccgt ggcctatgtt
      181 tcggccgtcg gggccgagca tcctccggca gagccgtcac gcgccgccgg gtgagggccg
      241 tagcatttcg gccaaggtgc gcagcaggag gcggagcccc accggtttct ggaggtagcg
      301 ccagcccttt tcgcggatct gcggccagcg cgttcggtcg tcggtatagc cgctgaccat
      361 gagcaccagg agggcgggct tttccttttt cagttcctcc acgagatcga agccggatcg
      421 gtgcgggaga acgacgtcgc tgcaaacgag ggcgaagtgg cccttttccc tcgtaaagat
      481 gtccacggcc tcctccgcgc tggcggccaa cagcgtctcg tacccgttct ggcccagcat
      541 gtgggccatg aggttgcgga cgctgtcgtc atcttcgacc accaggatcc tctcgccgca
      601 gccgcgcaac gtttccaggg gcggctccgc cgagacgcgg gccacggcct ccgccgtagc
      661 cggcaggtag atgtggaaca cggtgccggc gcccagctcg gagtacaggt ggacccagcc
      721 ctcgtgctgc ctgacgatgc cgtacacgga ggagaggccc atgccggtcc cctttcccgt
      781 cggcttggtg gtgaagaaag gctcgaagac atggcgttgt atttcttccg acatgccggt
      841 gcctgtgtcg cccaccgaaa tccggatgaa acgaccgacc cgcgcgtccg ggtgcgcggc
      901 aaggtcctcc tccgcgaagg tcaagttggt tgtcgagatg gtcaatgagc cgcccctcgg
      961 catggcatcg cgggcgttga ccgccaggtt gaggatcacc tgttccatct ggccggggtc
     1021 ggcgcggacc gaccagaggc gaggctccat gttgaggttc agggccacgt cctcgccgat
     1081 catgcggcgc agcattttgc ccatgttctc gatcaactcg ttgaagtcca aggccttctg
     1141 ctccagcacc tgccgacggc tgaacgcgag cagctgccgg gtgagcgcgg cggcggactc
     1201 ggcggccttg cggacctcga gcatatcctc ccgctgggca gcttccggtt cgtcctcgga
     1261 caaggccaac tccgtgaagc cgcggatgga ctggagcagg ttgttgaaat cgtgcgcaat
     1321 cccgccggcc agggtgccga tcacttccat cttctgggcc tgggccagct gtttctcgat
     1381 gtccagttcg cgcgacacgt cccttttcac tgccacgtaa ttgacgattt tcccgtgggc
     1441 gtcgtggacg ggcgaaatgg atacgtcctc cgtatagagc acgccgtcct ttctccggtt
     1501 caccaggcgg ccggtccata tggcgccgct cgaaatcgtg tcccaaagct gccggtagaa
     1561 atcttcgccc ttgcggccgc ccttaaggat gcgcgggttc ttcccaatga cctcttcccg
     1621 cgcgtagccg ctgaccctcg tgaacgccgg gttgacgtac tggatgttgc cctgggcatc
     1681 cgtgatgacg acggcctcgc cggcctgctc gatggcggac gccaggcgcg ccctttccgc
     1741 ctggcccctc cgctcgtcct cgaggatgct caagagcacg atccgggcgc ggtcgctctc
     1801 ggccgccaat tgaacggcct tctcctcggc ccgcttccga tccgtgatat ccacgtccat
     1861 gcagaacacc tcgctgaccc gccctttgtt gaacaccggg aacataacgg aatacagcca
     1921 gcgctcctcc ccgttcttcg cggtcacccg ccgttccgcc ggcggggaac cctgccccgt
     1981 ccgccatatc tcggcgatgg tgttctggcc gccggtctcg gggtcctcct ggaggagatc
     2041 ctgcatgcga ttccccacgg cctcttcctg cgtagcgccg aacaggcggg tcgcggcctg
     2101 gttccattgc agaatcgtcc cgtcgggcgc gtagctttcc acggcgacca ggggattgga
     2161 ttccaccacc gcggaaaacc gggccagggc ttcgcggacc atctccgagg catgtttctg
     2221 ctccgtgatg tcatcgatag cgagaaccgc gcagcgctgc ttgttcatca cgacggaact
     2281 ggcccggaag ctcaaccagc gcggggcggg gtgagattcc atgcacacct cggcttcgcc
     2341 gtgccgaggg gcgggatccc catcctccgc cagggcgctt cggatggcca ggttcagccg
     2401 gcaagccggg cacaggtcgg tgctgccaca accccgggga tccctctccc ggttcacgca
     2461 ccggatgaaa tcaccgcact tcagcgcgcc cagggaacct tccgagcgtc ggaccatctg
     2521 ctccgcgacc gcgttgaaaa agaggatgcg ctcgtcggcg tcgatgacga gcatgccgac
     2581 gggagaagag gtcagcaccg actgcaggtt gtcgcggctt tccagcagct gctcctgccc
     2641 gtgccggacc ctccggttgg cgtggtgcag cccgatcacg cccaggccca gcaggatcgc
     2701 cacggcggcc agggtcaacg gcagcgccgc ccgcgccatg acgatctttc tccaatcccg
     2761 cgcatcgata tccatgccga agaccgccag cagcctgccg gtctccggct gcagcatggg
     2821 cacaaatgcg ctgacccagg ttccccagcg atccgtaaac ggcccctcgc tggactcctt
     2881 ctcgctgtca aaaacagccg tgacgaatcg gctcacttcg tcatacgttt gcccgggcgg
     2941 cgaatagtcc ggcgagtccg cgggctcgga atccacgaaa aagaagatca tcccgtccgc
     3001 ccggcgaccc atgaggtaga ggaatcggca tcggtcattg gccttccgca tattcgtcaa
     3061 ctgttccttc agccgcaggt agtcgggcga acgcaggtcg gcgtccgatc cctgaaggtt
     3121 ctgcacccgc tcgacattga tggccaaggt ggcgacacgc gcctgctgga tcaattcccg
     3181 gcgcagcagg ccgtcggccc ggcgtgacgc ccacacggtc gcgcccgcga tcagcatgat
     3241 cgccgggagc gtcagcagcc acagcatgga ggagcggaat ttcatggccc tgtgggaaac
     3301 gatttcagtc gccccctcca cttccgtatc tcggcttctc gcccagcctc ttcagcaact
     3361 cgttcacctc ggccttgagc tcgatgaccc gcccttcccg tcccaaggtc gccgattgcc
     3421 agcggcgcaa ctcgttgagc tgttgccgca gcttctcctc cgcctgcttg cggtcggtga
     3481 tgtcgttgat gctggagagg acgaactttt cgccgcgcaa gttgatgatc ctggcggaga
     3541 agagtcccgt gatgagatcg ccgctcttaa cgcggaacgg gaactccacc ccgtccacga
     3601 cttcgccccg gcgaatcgtg tcgaccacgc tgctgcggtc ctccgcgcgc gtccacagtc
     3661 ccaattccag cgagctgcgc ccgagaacct cctcgcggcg gtatccggtg atcgagcaga
     3721 agccgtcgtt gacctccatg aaccggccgt cttcggggcg cgtgatggtg atggcatacg
     3781 gcgaggtctg gaaggcgatg gagaacttct cctcgctttc ccgcagcgac gcctcggccc
     3841 gcgcttgggc ctcccggctc tcccgctgct gcagggcctc cttcacggcg aacgggaggc
     3901 gggtcatgtt gtccttgagc acgtagtccg ccgcgccggc cttcatgcac tggacggcgg
     3961 tctcctcgtt gagcgagccc gtcagcagga tcaacggcgg gcggggatcc cgccggagcg
     4021 ccaactgcag cgctttcatg ccgttgaaac gcggcatgga gtagtcggag atcaccacgt
     4081 ccggccggaa ttcgtccagg gccttcaagt aatcctcctc cgtctctacg cgccgggact
     4141 caaaatccag atcgcctcgt cggagttccc gctgggccaa ctccacgtcc gccggcacgt
     4201 cctccgcgaa caggatgcga atgcgcgatg ccgttttcat gccgaggtct ccatcagccg
     4261 ttcgtcggtt gattcaacag gagccagtat aatcccaggt cccgcacgga ttcgagaaac
     4321 ttgtcgaaat ccacgggctt gacgatgtag ctgttcactc caagccggta gctttcgacc
     4381 atgtcttgtt cctctctcga cgacgtcaaa accacaacgg gaatcgtccg ggtgtgctca
     4441 tcgcctttga cggcgcgcag cacctccagc ccgttcactt tcggaagctt cagatccagg
     4501 aggatcaccc gcggccgggt ttcaatgtcc cggtccttga aggccccccg cgcgaacacg
     4561 aaatcgagcg cctcggcgcc gtcggctacg tggacgatgt ggttggccag gttgttcttc
     4621 ttcagggccc ggagcgtcag ttccgcgtca ttcgggttgt cttcgaccag gaggatttcg
     4681 acttcgttag ccttattcat ggggttcctt tcagtcaccc gtaatccatg gcccgtcccc
     4741 ggtcattaca ccctcccctt cggcagcgcg aaccagaacg tggcgccttc gtcaaccttt
     4801 ccctccgccc ccacccggcc gccgtggcgc tgtacgatgc gctgcacgat ggccaggccg
     4861 atgcccgagc cctcaaactc ggtggtcttg tgcaaccgct ggaaaatgcc gaacagcttg
     4921 gcctggtacc ggggatcgaa cccgaccccg ttgtccttca cgaagtagat ctgctcctcc
     4981 ttttcagccc gcgcgccgat ctcgatgcgc cgcttgtccc gggacgcgct gtacttgatc
     5041 gcgttactca gcaggttaaa ccagacctgc cgcagcaacg tcgggtcgcc gcggaccgcg
     5101 ggcaggttgg gcagtatgaa ttcgctccgc gccgcctcgt cggaagggag cagttcctcg
     5161 tacaccgaac gggccaacgt cgccatgtcc accgggacgg atctcacccc ggttcgcgtg
     5221 gcgcgggaca gggccagcag gtcggtgata agacggtcca tctgccgagt attttcgcga
     5281 acgacggtca gcagccgcgc ggcctcggtg tccatcttgc cggcgtagtc ctccagcacg
     5341 atccgggaga agccatcgat cgcgcgcagc ggcgcgcgca ggtcgtgcga tacggaatag
     5401 gcgaacgcct ccagttcctt gttcgaggcc tccaactggg ccgtgcgcaa tcggacacgc
     5461 tcctccaact cggcgttgag ccggcggatg tcctcctcgg cttttttccg ctcggtgatg
     5521 tcccacacgg tcgagatcat cagaggcttg cccggcacgg ggatatttct cgcggtaaca
     5581 aaggcggttt cctggcccgc gcgggtgatc ggcacgtcgg gccagtacat ccgttccggc
     5641 tggccgctgg cgcagtcctc gaggactttc ttcctgatcg cttcgcggaa ttccggatcc
     5701 tcgtagacgg cgtcccagaa cccgtcgtgg cgggccagcg cctcgcgtgt ggtccggtag
     5761 aagcgcggga agttgtcgtt catgtagtgg aaggacaccg ccgggtccac ggaattgacg
     5821 gccacgccga ccggcaggtt gtcgaggacc gccgtggtga aggcctcgtt ctggcgcagc
     5881 gcctccgagg ctcgccgcag ttccgtgatg tcggagagga tgccattgat gccggaggcg
     5941 ggatccttct cgcggcgccg ccggctcgtg gagcgcaccc agcgctccga gccgtcccgg
     6001 gccagcaccc ggaattccga ggcatgatac cggcccgcca gcgcatccgc aaagtaactc
     6061 tggaccgcag ggaggtcctc cggatgaatg aactccgcga acgagcgccc gatcacctcc
     6121 tccggctcat agccaaacag gaaccgggcc gccgggctga cataggtgag gaggcccgcc
     6181 cccgatacgc tgaacaccat gtcgctcaag ttctcgacca tgtcccggta ttgctcttcg
     6241 ttgacgcgca gcgcctccac catccgggtc cgttccgtct cggcctcgag ggcatgcaag
     6301 gcgaagccga tgtcgccgac gagttcccgg aagagtgatt gttcctccgc gtcgtccgcc
     6361 atcacgcgcg ccagcgccac gacgatgacc ccgtagttac gttccccgtg ccgcagcggg
     6421 cccgccagga ctgccgtgtc gcagtcggtg tgggccatcg gacaatgcac gcagtctcgc
     6481 ttcgtgtcgt gcatgaccac gatctccgac cgggcgaggg ccaggcggca gcactccgga
     6541 acctggcccg cctccaggtg tgtccggagg gcggataagc tcgcgtccac acccgagccg
     6601 gccgctgggc gcagccgccc gtccgcctcg agcaggctga tccaagcgga gcggtagccc
     6661 cgggtttcgg cgagaatagt gcacgcgctc cgcaccaggg cctccctgtt tttttcgtgc
     6721 gtgatcagct ggttcacgtt gcggaccgcc tggagcactt cggtcaggtg ccggaggcgc
     6781 tcctccaccc gcttccgttc cgtgatatcc accgagagga tgaacaagcc ctcgggcacg
     6841 cgttggaggc tcaactcgaa ccagcccgtt tcaccgtcgg gataggcgaa gaagttctcg
     6901 aagcggcgcg gctgacgatg tccatatcag gttggataaa ttgcggcttc caagccactg
     6961 aggcaggggt gagcgcatac ccgaggatcg gtttgggggc ggtaaacccg gtgagattga
     7021 acgaggagta cgaggagctg atggacggcg cgggcgtttg gatggagttc tacaatgaca
     7081 aacgatatcc agaaacaggc taccttgcaa catccaagtt ggaagcgctg aacaggttcg
     7141 ccgagatagg cggttttgtc gagggttctg cggtaaagag aggggttttt gcaggcataa
     7201 ataagaatgg cctgcttgcg gcactgatag ccgagatggg aatgcagtat caatccaagg
     7261 gcgaagcagt tgatcgcttg aagggattgg ctgacgccac gaagagagac gtgcagattt
     7321 acctgaaccg aataatgctg ggttagcagc aagcgacgcc cccctttgca aacatttttt
     7381 tgtctgatta atcaatccaa gagcagtgaa tcggatgagg acaatatggg cgccttggcg
     7441 ctc
//
LOCUS       DAOOTK010000141         7191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6472_length_7191_cov_4.737528, whole genome shotgun sequence.
ACCESSION   DAOOTK010000141 DAOOTK010000000
VERSION     DAOOTK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6472_length_7191_cov_4.737528"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PK634_12465"
     CDS             complement(<1..1445)
                     /locus_tag="PK634_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOW98591.1"
                     /translation="MNFRVVFHLVSHMLLVIAGGMAAAWGVSLYAGDPSLAQLALGFS
                     CLGTLVSGLILKMATRGDTDLTRRDGFGIVTLGWLFASAFSALPFMLSGIIPHPVSAF
                     FEAMSGYTTTGATVIPVLEGLPRGILFWRALTHFFGGMGVLVLCVAILPFLGVGGMQI
                     FRAEMPGPSKDRLTPRITTTAKWLWGVYVFFTAIETGLLRLGGMSWFDAVCHSFATMA
                     TGGFSTRTVSIMAYQSPYIEAVITVFMFLAAANFALHYRALQGKVGSYFRDPEFRFYL
                     ALWTGSALFVSLILHTRLGMGALHALRLGFFQVTSIMSTTGFATADFERWPFVLKFTL
                     ILLMFVGGCSGSTGGGIKVVRLFVLAKAVGREIRLFMQPQAVLRVRLGEERLEPEVVS
                     SIMAFVAIFVLLFLAGTAVMCFFTPDFPTAASSVAATLGNIGPGLGLVGPMSTYATIP
                     PAGQAVLIVCMLLGRLELYTVLVMLLPGFWK"
     gene            complement(1449..2897)
                     /locus_tag="PK634_12470"
     CDS             complement(1449..2897)
                     /locus_tag="PK634_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOW98592.1"
                     /translation="MNGRAVAHLITYLLFMIGAGIAACWGVSLYFHDPAPAPFALGLS
                     AAITLAVAAIVHLATRGAIELSWRDGIGVGTLGWLAAAAFGSLPYILSGVIREPVSAL
                     FETISGFTTTGASVLATLEPLPKGILLWRATTHFLGGMGILVLCVAILPILGGGGMQL
                     YRAEVAGPSKDRMTPRIASTAKLLWGVYVLLTAAEIVLLRLGGMPWFDAVCHSFATLA
                     TGGFSTRTVSIAAYNSLYIEIVITVFMLIGAVNFALHYRLLRGQVRGTFRDSELKFFL
                     GFWLVGCLVVTLNTWRAVYPAFGDALRNSVFSFTSLATTTGFATADFDQWPATSKIVL
                     LLAMIAGGCVGSTGGGIKHMRILVALKKIARRVRLFMYPQAVMQIKVNGETVEDEIVS
                     MILGYIVLYLVTMAIASTVMTIFTKDLLAAVSSVIATMGGVGPGLGLVGPLQNYGPVP
                     AGGKLVLILCMLLGRLEFYTFLALFFPSFWKK"
     gene            complement(2911..4260)
                     /gene="trkA"
                     /locus_tag="PK634_12475"
     CDS             complement(2911..4260)
                     /gene="trkA"
                     /locus_tag="PK634_12475"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOW98593.1"
                     /translation="MRVLIIGSGNAGQNLAAKLCDEKHDVVLVDHRQEPLTEIQSHLD
                     IMTVRGPGSSPATLEKAEIRKADLLVAVTDRDEVNLLACVMAHAAGVAHKVARVSNLD
                     YIRPPEGYDLRKLGIDLIVSQKEECAYELFNVLRMPGTLEAVDLLEGRVLSAGIRVHM
                     DSPLLREPLKAFPKPELLQSIRFIAGKRGNELLVPRGDSQFMVGDEIYFVGPPDDVNA
                     FLEWAWPEHSSFQKVVIAGGGDVGVRLAQLLEKTSMQVVLVEPDAERAEYCSGMLGRT
                     LVIRGDPMSDETQAEAGLGQNTAFVAATPDDESNIIGCLLAAKLGARFTLAQVNKPEY
                     VPIIDSLSLLDHVVSPPLSMINAILHFVRGKHVKAAALFHKLPGELLDVVLSARGKWV
                     DKAVKDISLPAGVTIAAGLRHDKVLGVTGDLVLAAGDRLVLFSPPDVVAKLSAQFKK"
     gene            complement(4408..6912)
                     /locus_tag="PK634_12480"
     CDS             complement(4408..6912)
                     /locus_tag="PK634_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98594.1"
                     /translation="MMRPGLPAALVALALSARAQTHRYVDAAAGSDSASGLTPTQAWR
                     TIQRAATNAGAGWIIHVAPGAYRETVGPTNNGAAAAPIVFRGETSNDARVILSGAVSS
                     RTLTWVRLTNNSIGLPGGVAPSNIYYTSLAGWTTRPSRITWVDGTNRAALPLAREPDR
                     GVTTDWKYHENWWQADGGSTDQLWDATHDPAGAYPDVQTGHLGWINGFTNSFLTGAVV
                     VATDHQQAHDTYRRTITSHSASSGLVRFAASTLYTLGSNTKYYVEGPPQCLDRIGEWS
                     LVRIGTTNRLYLWPPNNSHPTNLDLEIARRGIGFNLQHRSHIRVEDMVIQDLNNLEGG
                     YTGDEGAFWIQNWDEDSVGLVFDRLTIRDVPLAFRMYANAELGMISNLTVQSSDIGPC
                     DNPAILTLFDTGATARVGISTLLIRSNTFHHLGFASRIDQGIGLLITAPHRLLFEGNH
                     VHDVMQNGLVLAYGEEADSLILGNLFERCALGAGDCAGFKVWASGRASSRILVMRNVF
                     RDNPGWTFPVSQINWWETSRGRFAGFGTYTDIVRSPATDDYAVVFYRNEAYRNGYAGF
                     FIAHSRDMALFNNVCAGQPHGISLDGQLSTMPGVNSNCLAFNNLLLIHQTNASLYADS
                     GISIRAPSSEMTRAQCDYNVYQVRGDGATAMRHYHRWDSEAESWRHNLYTNTAEIRDR
                     TPWEDHGQDAPADADMVADEETGDFNLYLNSPAVDGAIPPQIALDLVTRLESVLGMEI
                     EDEALYGAGYDAGPLECHPESIEFTGATLSEDSLPVFTWSSVYGAAYSVESSTNRVHP
                     DWVIITNLSSSGYATSFTNSAVQGPLLLRTRLDGPD"
BASE COUNT         1325 a         2227 c         2481 g         1158 t
ORIGIN      
        1 tttttccaga agccgggcag cagcatgacg aggaccgtgt agagttccag gcggccgagc
       61 agcatgcaca cgatcagcac cgcctgcccg gcgggcggga tcgtggcgta ggtgctcatc
      121 gggcccacca ggcccaggcc cgggccgatg ttcccgagcg tggcggcgac ggacgaggcc
      181 gcggtgggga agtccggtgt gaagaagcac atcacggccg tcccggcgag gaacagcagc
      241 acgaagatgg ccacgaaggc catgatgctc gagaccacct ccggctccag ccgctcctcg
      301 cccaggcgca cccggagcac ggcctgcggc tgcatgaaca gccgtatctc gcgcccgacg
      361 gccttcgcga gcacgaagag ccgcaccacc ttgatcccgc cgcccgtgga gcccgagcag
      421 ccgccgacga acatcagcag gatcagcgtg aacttcagga cgaacggcca ccgctcgaaa
      481 tcggccgtcg cgaagccggt cgtgctcatg atcgacgtga cctggaagaa gccgagccgc
      541 agggcgtgga gcgcgcccat gcccaggcgc gtgtgcagga tcagcgagac gaacagcgcg
      601 gagccggtcc agagggccag gtagaagcgg aattccgggt cccggaaata cgagccgacc
      661 ttgccctgga gggcccggta atggagcgcg aaattggcgg cggcaaggaa catgaacacc
      721 gtgatcacgg cctcgatgta ggggctttgg taggccatga tgctgaccgt gcgggtggaa
      781 aagccacccg tggccatcgt ggcaaagcta tggcagaccg cgtcgaacca ggacatgccg
      841 ccgaggcgga ggagccccgt ttcgatggcg gtgaagaaga catagacgcc ccacagccac
      901 ttggccgtgg tcgtgatgcg cggcgtcagg cgatccttcg agggccccgg catctccgcg
      961 cggaagatct gcatgcctcc gacaccgagg aagggaagga tggcgacgca gaggaccagc
     1021 acacccatgc cgccgaagaa atgggtcagc gcgcgccaga agaggatgcc ccggggcagg
     1081 ccttccagca ccgggatcac cgtggccccg gtggtcgtgt agccggacat ggcctcgaaa
     1141 aaggcggaga cggggtgggg gatgatgccg gacagcatga acggcagggc gctgaacgcg
     1201 gaggcgaaca gccagccgag ggtcacgatg ccgaacccgt cgcgccgggt gaggtccgtg
     1261 tcgccccgcg tggccatctt caggatcagc ccggaaacca gggtgccgag gcaggaaaag
     1321 ccgagggcca gttgagccag ggaggggtcg ccggcataca gggagacacc ccaggcggcg
     1381 gccatgccgc ccgcgatgac gagcagcatg tgcgatacga gatgaaagac gacccggaaa
     1441 ttcatccgct atttcttcca gaagctgggg aagaatagcg cgaggaacgt gtaaaactca
     1501 agccgtccca gcagcatgca caggatcagc accagcttgc cgccggccgg gacgggcccg
     1561 tagttctgca ggggaccgac caggcccagt ccggggccca cgccgcccat ggtcgcgatc
     1621 accgaggaca cggccgcgag caggtccttc gtgaagatcg tcatgacggt cgaggcgatc
     1681 gccatcgtca ccaggtacag cacgatgtag ccgaggatca tcgagacgat ttcgtcctcc
     1741 accgtctccc cgttcacctt gatctgcatc acggcctgcg ggtacatgaa caggcggacg
     1801 cggcgcgcga tcttcttcaa ggcgaccagg atgcgcatgt gcttgatgcc gccgccggtc
     1861 gagccgacgc aaccgccggc gatcatggcc aggagcagca cgatctttga cgtcgcgggc
     1921 cactggtcga agtccgccgt cgcgaagccg gtggtcgtgg ccagcgaggt gaaggaaaag
     1981 acgctgttgc ggagcgcgtc gccgaaggcg ggatagaccg cgcgccaggt gttcagggtc
     2041 acgacgaggc agccgaccag ccagaaaccg aggaagaact tcaactcgga atcccggaat
     2101 gtcccgcgca cctggccgcg caggaggcga tagtgcaggg cgaagttgac ggcgccgatc
     2161 agcatgaaca ccgtgatcac gatctcgatg tagaggctgt tgtaggcggc gatgctgacc
     2221 gtgcgggtgg agaacccgcc cgtggcgagc gtggcgaacg agtggcacac ggcgtcgaac
     2281 cagggcatcc cgccgaggcg caggaggacg atctcggccg ccgtgagcag gacatagacg
     2341 ccccacagca gcttggccgt gctggcgatc cgcggggtca tgcggtcctt cgacggaccg
     2401 gcgacctcgg cgcggtagag ctgcatgccg ccccccccga ggatcggaag gatggcgacg
     2461 cagaggacca ggatgcccat gccgccgagg aaatgggtcg tggcgcgcca gagcaggatg
     2521 cctttcggca acggttccag cgtggccagc accgaggcgc ccgtggtggt gaaccccgag
     2581 atcgtctcga acagggcgga caccggctca cggatgacgc cggacaggat gtagggaagg
     2641 gagccgaagg ccgccgccgc gagccagccc agggtgccga cgccgatgcc gtcccgccag
     2701 gacagctcga tcgcgccgcg cgtggccagg tgcacgatcg cggcgaccgc cagcgtgatg
     2761 gccgccgaca ggcccagcgc gaagggggcg ggcgccgggt cgtgaaaata cagcgagacg
     2821 ccccagcagg ccgcgatgcc ggcgccgatc atgaacagca ggtaggtgat caggtgcgct
     2881 acggcccggc cgttcatggc tcgctcccgc ttatttcttg aactgggcgg acagcttggc
     2941 cacgacatcg ggcggcgaga acagcaccag ccggtcgccg gccgcgagga ccaggtcgcc
     3001 ggtgacgccg agcaccttgt cgtggcgcag cccggcggcg atggtcacgc ccgcggggag
     3061 ggagatgtcc ttgacggcct tatccaccca cttgccgcgc gcggagagca ccacgtccag
     3121 cagttcgccg ggcagcttgt ggaacagggc ggcggccttg acgtgcttgc cgcggacgaa
     3181 atgcaggatg gcgttgatca tggacaacgg cgggctgaca acgtgatcga gcaggctcaa
     3241 gctgtcgatg atcggcacgt actccggctt gttgacctgc gccagggtga agcgcgcgcc
     3301 gagcttggcg gcgagcaggc agccgatgat attgctctcg tcgtcgggag tggccgcgac
     3361 gaacgcggtg ttctgcccca gcccggcctc ggcctgggtt tcgtcgctca tcgggtcgcc
     3421 gcggatgacc agcgtgcggc ccagcatccc ggaacagtac tccgcgcgtt ccgcgtccgg
     3481 ctcgaccagc acgacctgca tggaggtctt ttccagcaac tgggccaggc ggaccccgac
     3541 atcgccgccg cccgcgatga ccaccttctg gaagctcgaa tgctccggcc acgcccactc
     3601 caggaacgcg ttcacgtcgt cgggcggacc gacgaaataa atctcatccc cgaccatgaa
     3661 ctgcgaatcg ccgcgcggca ccagcagttc gttcccgcgc ttgcccgcga tgaagcggat
     3721 ggattggagc agttccggct tggggaaggc cttgagcggt tcccggagca gcgggctgtc
     3781 catgtgcacc cggatcccgg ccgaaagcac ccggccctcg agcaagtcca cggcttccag
     3841 cgtgccgggc attcggagga cgttgaacaa ctcgtaggcg cactcttcct tctggctgac
     3901 gatcaagtca atgcccagct tgcgcaggtc gtacccctcg ggagggcgta tgtagtccag
     3961 gttcgagacc cgggcgacct tgtgggccac cccggcggca tgagccatga cgcaggccag
     4021 gagattcacc tcgtcgcggt ccgtcacggc caccagcaga tccgccttgc ggatttcggc
     4081 cttttccagc gtggcggggc tcgatccggg cccgcgcacg gtcatgatat ccaggtgcga
     4141 ctggatttcg gtcagcggct cctgccggtg atccaccagc accacgtcgt gtttctcgtc
     4201 gcagagcttg gccgcgagat tctggccggc atttccagat ccgatgatga gaaccctcat
     4261 gcaatcctcc tgtccagagg cgggcgaaaa tgcgcatctg ccttaaaata aaggaattgc
     4321 aacatataga acggccgacc cttttagtca cggacaggcc gctaccgtgg attctgcact
     4381 gtatccggta cctgcagaaa atcaagatca atcgggcccg tccaggcggg tccgaagaag
     4441 gagggggcct tgcacggcac tattagtaaa gctggtggcg taacccgatg atgaaagatt
     4501 agttatgatg acccagtctg gatggacgcg gttggttgag gactcgacag aataggcggc
     4561 cccatagacg ctggaccatg tgaagaccgg caaactgtcc tcggaaagcg ttgctccagt
     4621 gaactcgata gactcgggat ggcattccag cgggcctgca tcgtaccctg ctccatacag
     4681 ggcttcgtct tcaatctcca ttcccagaac ggattccagc cgggtgacga ggtccaaggc
     4741 gatctgcggc ggaatcgcgc cgtccaccgc ggggctgttc aagtacaggt tgaagtcgcc
     4801 ggtctcttca tcggcaacca tatcggcgtc tgccggcgcg tcctggccgt gatcttccca
     4861 cggggtccgg tcgcggattt ccgctgtgtt ggtgtacaga ttatgccgcc acgactccgc
     4921 ttccgaatcc cagcggtgat agtgccgcat ggccgtcgcc ccgtcgcccc tgacctggta
     4981 cacgttgtag tcgcactgcg cgcgggtcat ctcgctgctg ggggcccgga tggagattcc
     5041 ggagtcggcg taaaggctgg cattggtctg gtgaatcaag agtaaattat tgaaggccaa
     5101 gcaattggag tttacgcccg gcatcgtgga cagttgtccg tccaacgaaa tgccatgagg
     5161 ctggccggcg cacacgttat tgaacagggc catgtcgcgg ctgtgggcga tgaagaaccc
     5221 ggcgtaaccg ttgcggtagg cctcgttccg gtaaaaaacg acggcatagt catccgtcgc
     5281 cgggcttcgg acgatatctg tgtatgtccc gaacccggcg aaacggcccc ggctggtttc
     5341 ccaccagttg atctggctca cggggaaggt ccagcccggg ttgtcccgga acacattccg
     5401 catgaccagg atccggctgc tggcccggcc gctggcccag accttgaagc cggcgcaatc
     5461 tcccgcgccg agcgcgcagc gctcgaacag gttgccgaga atgaggctgt cggcttcctc
     5521 cccgtacgcc aggaccaggc cattttgcat gacgtcgtgg acgtgattgc cctcaaacag
     5581 caggcggtgc ggggcggtga tgagcaggcc gataccctgg tcgatccgcg atgcgaaccc
     5641 gaggtggtga aaggtgttgc tccggatcag cagcgttgag atgcccaccc gggccgtcgc
     5701 gccggtgtcg aacaacgtca ggattgccgg gttgtcgcac ggcccgatgt ccgaactctg
     5761 gacggtcaga ttgctgatca tgcccaattc ggcgttggcg tacatgcgga aggcgagcgg
     5821 gacatcccgg atggtgaggc ggtcgaagac caggccgacg ctatcctcgt cccaattttg
     5881 gatccagaac gcgccctcgt cgccggtgta cccgccctcg aggttgttga gatcctggat
     5941 gaccatgtct tcgacccgga tatgggaccg atgctggagg ttgaacccga tgccgcgccg
     6001 ggcgatctcc aggtcgaggt tggtcgggtg gctgttgttc ggcggccaga ggtagagccg
     6061 gttcgtggtg ccgatccgta cgaggctcca ctcgccgatc cggtcgaggc attggggcgg
     6121 gccttcgacg tagtacttgg tgttggaccc cagggtgtac agtgtcgagg ccgcgaatcg
     6181 cacaaggccg gacgaggcgc tgtggctggt gatggtccgg cggtaggtgt catgggcctg
     6241 ctggtggtcg gtggcgacga ctaccgcgcc cgtgaggaag gaattcgtga agccgttgat
     6301 ccagcccagg tgcccggtct ggacgtccgg gtatgcgccg gccgggtcgt gcgtcgcgtc
     6361 ccagagctgg tccgtggacc cgccgtccgc ctgccaccag ttctcgtggt atttccagtc
     6421 cgtggtcacg ccccggtcgg gctcccgggc gaggggcagg gcggcgcggt tggtcccgtc
     6481 cacccaggtg atccgggacg gccgagtggt ccagccggcg agcgaggtgt aatagatgtt
     6541 gctgggcgcg acgccgcccg gcaggccgat cgagttgttg gtcagccgga cccaggtcag
     6601 cgtccgggag gagacggcgc cggacaggat gacccgggcg tcgttggagg tttcaccgcg
     6661 gaagacaatg ggcgccgccg cagcgccgtt gttggtcggg ccgacggttt cgcggtaggc
     6721 gccgggcgcg acatggatga tccagcccgc gccggcattg gtcgcggcgc gctggatcgt
     6781 gcgccaggcc tgggtgggcg tcagcccgga ggcgctgtcc gatcctgccg cggcatccac
     6841 gtagcggtgc gtctgggccc gggccgaaag cgccaaggcg acgagggcgg ctggcaggcc
     6901 gggtcgcatc attcccagcg tacccgtttt gacgcggtct gtaaaaaaca agccggcgct
     6961 tcggacggct ccgtcgtgcc ggggcccatc cctgcatttc ccggtcggga attgcgcgca
     7021 gcattatttc cacactttgc aaagtgtgga aatattctgg aggcgccgtg gcccggccgg
     7081 cggggcgaca gacgcttctt tacgccgcgg gagggcggcg ttatgctggc cttccaactc
     7141 gcacgggggg aatgggccat gaaaatgctg ctggtcggtg tcgcgctcgc g
//
LOCUS       DAOOTK010000142         7165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6496_length_7165_cov_4.724473, whole genome shotgun sequence.
ACCESSION   DAOOTK010000142 DAOOTK010000000
VERSION     DAOOTK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6496_length_7165_cov_4.724473"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK634_12485"
     CDS             <1..453
                     /locus_tag="PK634_12485"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOW98595.1"
                     /translation="SNFRPLADLFTALSVPGERLLRVNTSLPLDEAAEQYHRDLQALL
                     QGGAHFTLGLLGLGADGHTASLFSPADVRRGAGRLAVAVRRPNPPHRVSVTPGLLQHV
                     EEIVFLVSGSDKTAVVRKLLDDPESLPAGLAVAGCRNVSLWMASTPAP"
     gene            450..2195
                     /locus_tag="PK634_12490"
     CDS             450..2195
                     /locus_tag="PK634_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW98596.1"
                     /translation="MNRDATQTPAPGGRGSLELMRLFLGLYPWRSLVTVGCLLLSGAA
                     EALGLGAILPLAGLLMRREIPIDLPVARWACRAIDLLGLPASVPGLLAVIVTAFLVKA
                     LISFLTARYVAHTAVDIAADFRLDLLRALMKARWGFFLGQPSGRLAHTMGFATEQAAA
                     IYLYTGRILAGVVQALTYAALAFAVSWRVTLLALAAGAAAMSVFHGFVKQVRRAGARS
                     SALFESVNATLVDSLQGLKPLKAMACEDRVADLLEADIRDLKRWQRKTETSRAALRTL
                     REPLQLAAAAAVFFLAWSLWNVGVEVLSLCLLLLVQVMGRINTLQVMLQDLVRYETMF
                     WRLRDTIAQAAAGAEAPPAGQAPVLARGLQLDRISFRRGETPVLRDISAELPAGRITA
                     LAGPSGSGKTTLADLLVGLITPDAGEVRLDGVPLGRLDLRRWRERVGYVPQEPFLFHD
                     TVRRNVTLGDPALAADDVERALRAVAAWDFIAALPKELDTVVGERGAKLSGGERQRIA
                     IARALVRRPALLILDEATTGIDPATEDAILAALKQLPDHPTILAISHRSGFSRVADAA
                     YTLEDGRLTPGARAG"
     gene            complement(2263..5253)
                     /locus_tag="PK634_12495"
     CDS             complement(2263..5253)
                     /locus_tag="PK634_12495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98597.1"
                     /translation="MKILMTCLLDWETPSAGRIHFGSLARAFAAEGHEVIGVIPARRD
                     RARDPAPFARVYATPRFNEGYAGQLLRAAAHLGVLARAIRRERPDAIYVRFRSCSPLS
                     CAAARLCARGAVVVGEHNTWASAYLEVSGYSTAVNALSRRNQLLAARSAHRIRTSTRH
                     LKNILVEQGIEAGRVFVAGMGADLDRFRPLPRDESLARAGLDPSARYVGYAGSLNRWQ
                     GVDVLIESLRHLPGDVRVVILGEGPQADNLRRQAAQAGLSDRVLFKGHVPHEDMPWWI
                     NCFEVGVGALRTQARGRMLGTPMKLAEYAACGAPAVAASVEGVEILEQRAAVIRVPSE
                     DARAAAEAIRRLLREPAERRSMGLAARAVAEECLSWTAIARQVLAHFLPIPAGPDRRS
                     ASDLRRLAGAGLPAGRAFTYLYRRHGIYLPRAARSVWVVGERPADFEAAGPLIRRLRE
                     LQPKHRLVVLSPDPGTFDWLCARYAGDTALPFPRDTRRAVDRFVAALAPRLVIRLGRD
                     SVPGARWAAPLAGAGTPVVTVRAGGADPDAGALIREDLSFEVGLGLPVPAGAYLRREL
                     GLSEEAPVILAEQVCPGEESILLAAWCRVREARPDAVLLLEPRRRGQTGSILRLARRY
                     GLAARRRSQPVRAETPAVLVLDRAAELPALYAVASVAVLGGSFTAGGPVANPVCPARF
                     GVPVVTGPGSRDGVEKFVREGAAIRMPSANLAESLLTLLGDPARRKALAESAGRLVAA
                     NEGATMRAWSALAPFLPSGPREPDTQGWRVKTRVDRLSDTAFGQWLAGRRSGRRLDTW
                     EELRERLGRPRAILCLGNGPSSEDPAIRDICHDCLMRINWRWKGRGLLDRPDLVMVGH
                     PRTLQEAPPCVFGFGRVEWERAMLIRHLLMGGLRPVEFFTLERVASILRPTDWHARPT
                     NGALLVAIAAALQPDELILAGYDLFRHPDGRYPGDVHSQNEPAQVHDRDADVEVIARA
                     LAAYTGRVDIRSDILRAALDARPA"
     gene            5381..6097
                     /locus_tag="PK634_12500"
     CDS             5381..6097
                     /locus_tag="PK634_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98598.1"
                     /translation="MDLNVACVKWGTKFPADYVNRLRAMVRRNLPLPHRFVCLTDDPA
                     GLASGIEPLGLRGGLEYCWNKLELFRPGLFSGDDICLYFDLDVVITGPIDALLSFRPQ
                     DVFVGLYDWYRSRVPHYNSSVMRFQGGRHETLYTSLGEKLAAGRVRWAREYDAYLGSN
                     DKVVLWEDETRYGGDQEWISRYVYAPRELKEHSFPGGWLLSYRKHGRRELPRGCKVMV
                     FHGDPKPHEVENDYVREHWR"
     gene            complement(6313..>7165)
                     /locus_tag="PK634_12505"
     CDS             complement(6313..>7165)
                     /locus_tag="PK634_12505"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOW98599.1"
                     /translation="CMPGVLDGDPGEVVFGIGTILCSGMPVPAATRCIHVLGSGCGYG
                     DRAPDPRYRVWFVRGPRTAARLGVEDSKAISDPAMLLPRLMSAAGPAKARAPVFIPHI
                     DSALVCDWATPCALAGVRLVDPRTPAEAFIDAVRAAPLVLAESLHGAVVADAFGVPWV
                     PLASSDDILAFKWEDWLDTLALEYRPCRVQRRIPWGWTRLRGGLRRLADDLGLSRRAA
                     RTRVSSLLERQPRAENCADSLLNASRQTPFLSQEEVRSRIAERLAEAITAFARFAGKP
                     AVKDRSV"
BASE COUNT         1064 a         2586 c         2492 g         1023 t
ORIGIN      
        1 agcaacttca ggccgctggc cgacctgttc accgcccttt ccgtgcccgg cgagcggctg
       61 ctccgcgtga acaccagcct tcccctcgac gaggccgcgg agcagtacca ccgggacctg
      121 caggccctgc tgcagggcgg cgcgcatttc accctcggcc tgctcggcct cggcgccgac
      181 gggcacacgg cctcgctgtt cagccccgcg gacgtccggc gcggcgcggg ccggctcgcc
      241 gtcgccgtcc ggcgcccgaa cccgccgcac cgcgtcagcg tcacgcccgg gctgctgcaa
      301 cacgtcgagg agatcgtctt cctggtttcc gggtccgaca agaccgccgt ggtccggaag
      361 ttgctcgacg atccggagag cctgcccgcc ggcctggccg tcgccggctg ccggaacgta
      421 tcgctgtgga tggcctccac gccagcgcca tgaaccgcga cgcgacccag acgccggccc
      481 cgggcgggcg cggcagcctc gaactcatgc gcctgttcct gggcctctac ccatggcgat
      541 ccctcgtgac cgtcggctgc ctgctgctgt ccggcgcggc ggaagccctc gggctgggcg
      601 cgatcctccc gctggccggg ctgctgatgc ggcgggaaat tcccatcgac ctgcccgtgg
      661 cgcggtgggc ctgccgcgcg atcgacctgc tcgggctgcc ggcctccgtc ccgggcctcc
      721 tggcggtgat cgtcacggcc ttcctggtca aggccctgat ctccttcctg accgcgcggt
      781 acgtcgccca cacggcggtg gatatcgccg cggatttccg cctggacctg ctccgcgccc
      841 tcatgaaggc gcggtggggg ttcttcctcg gccagccctc cgggcggctg gcccacacca
      901 tgggctttgc caccgagcag gcggccgcca tctacctcta caccggccgc atcctcgccg
      961 gcgtcgtgca ggcgctgacc tacgcggcgc tcgcgttcgc cgtgtcctgg cgggtgacgc
     1021 tgctggcgct ggcggcgggg gcggcggcca tgtcggtctt ccacggattc gtgaagcagg
     1081 tccgtcgcgc cggcgcccgc tcgtccgctc tcttcgagtc ggtcaacgcg acgctggtgg
     1141 acagcctcca aggcctcaag ccgctcaagg ccatggcctg cgaggaccgc gtggccgacc
     1201 tgctggaagc cgatatccgg gacctgaagc gatggcagcg gaaaaccgaa accagccggg
     1261 ccgccctccg cacgctgcgc gagcccctgc agctcgcggc cgccgccgcg gtgttcttcc
     1321 tggcctggtc gctctggaac gtcggcgtcg aggtcttgag cctgtgcctc ctgctcctgg
     1381 ttcaggtcat gggccgcatc aacacgctgc aggtcatgct gcaggacctg gtccgctacg
     1441 aaaccatgtt ctggcggctg cgcgacacca tcgcgcaggc ggccgccggg gccgaggcgc
     1501 cgcccgccgg ccaggccccc gtcctcgcgc gcggcctgca gttggaccgg atctcgttcc
     1561 gccggggcga aacccccgtc ctccgggata tctccgcgga attgccggca ggtcgcatca
     1621 ccgcgctcgc cggcccctcc ggctccggaa agacgaccct cgccgacctg ctggtcggcc
     1681 tgatcacgcc ggacgcgggc gaggtgcgcc tcgacggcgt gcccctcggc cggctggatc
     1741 tccgccggtg gcgcgagcgg gtcggctacg tgccccagga gccgttcctc ttccacgata
     1801 ccgtgcggcg caacgtcacg ctgggcgatc ccgcgctggc ggcggacgac gtggagcggg
     1861 ccctccgcgc cgtcgccgcc tgggatttca tcgcggcgct tccgaaggaa ctcgacaccg
     1921 tcgtgggcga gcgcggcgcc aagctgtccg gcggcgagcg gcagcgcatc gccatcgcgc
     1981 gcgccctcgt ccgccgcccg gcgctgctga tcctggacga ggccaccacg ggcatcgatc
     2041 ccgccaccga ggacgccatc ctcgccgccc tcaagcaact gccggaccac ccgacgatcc
     2101 tcgccatctc ccaccggtcc ggcttctccc gcgtcgccga cgcggcctac acgctggaag
     2161 acggccggct cacgcccggc gcgcgggcag gctgactttt ccaatcattg gaaaaacgcc
     2221 gccgattttt tccaatgttt ggaaaaccgg cgggcggccg tctcacgccg gccgggcgtc
     2281 gagggcggcg cggaggatat cgctgcggat gtccacgcgg cccgtgtagg cggccagcgc
     2341 gcgggcgatg acctcgacat ccgcgtcgcg atcgtggacc tgggcgggct cgttctgcga
     2401 atggacgtcg cccggatatc ggccgtcggg atgccggaag agatcgtagc ccgcgaggat
     2461 caattcgtcc ggctgaaggg cggccgcgat cgccaccagt agcgccccgt tggtcggccg
     2521 cgcgtgccag tccgtcgggc gcaggatgga cgcgacgcgc tcgagcgtga agaattccac
     2581 cggccggagc ccgcccatca gcaggtgccg gatcagcatc gcgcgctccc actcgacgcg
     2641 cccgaacccg aagacgcacg gcggcgcctc ctgcagcgtc cgcgggtggc ccaccatgac
     2701 caggtccggc cggtcgagca ggccccgccc tttccaccgc cagttgatcc gcatgaggca
     2761 gtcgtggcag atgtcccgga tggccgggtc ctccgacgag gggccgttgc cgaggcagag
     2821 gatggcgcgc gggcggccca gtcgctcgcg cagttcctcc caggtgtcga ggcggcggcc
     2881 ggaccgtcgc ccggccagcc actgcccgaa cgcggtatcg ctcaaccggt ccacgcgggt
     2941 cttcacgcgc cagccctgcg tgtccggctc gcggggcccg gagggcagga agggcgccag
     3001 cgcggaccag gcccgcatcg tcgcgccttc gttcgccgcc accagccggc ccgcggattc
     3061 cgccagggcc tttcgccggg cgggatcgcc caggagcgtc agcaagctct ccgccaggtt
     3121 tgccgacggc atgcgaatcg ccgcgccctc ccggacaaac ttctcgaccc cgtcgcggct
     3181 tccgggtccc gtgaccacgg gcacgccgaa gcgcgccggg cataccgggt tggccacggg
     3241 tccgccggcg gtgaaactcc cgcccaggac ggccacggag gcgacggcgt acagcgcggg
     3301 cagttcggcg gcgcggtcca gaacaagcac ggccggcgtc tcggcgcgca ccggctggct
     3361 gcgccgccgc gcggccagtc cataccggcg ggccaaccga aggatggaac cggtctgccc
     3421 gcgccggcgc ggctccagga ggaggacggc gtccggccgg gcctcgcgaa cgcgacacca
     3481 cgcggccagc agtatgcttt cctcgccggg acaaacctgc tccgccagga tgacgggcgc
     3541 ctcttcagaa agccccaact cgcggcgcag gtaggccccc gccggaaccg ggaggcccag
     3601 cccgacttcg aaggacaggt cctcccgaat cagcgcgccc gcgtccgggt cggcgccgcc
     3661 ggcccgcacg gtcacgaccg gagtgccggc gccggccagg ggcgcggccc atcgcgctcc
     3721 cggaacgcta tcgcggccga gccgtatcac aaggcgcggg gcgagggcgg cgacgaaacg
     3781 gtccacggcg cggcgggtat cgcgcgggaa cggcagcgcc gtgtcgccgg cgtagcgcgc
     3841 gcacagccag tcgaacgtgc cggggtcggg agacaggacc acgagccggt gtttcggctg
     3901 gagttcccgc agcctccgga tgagcgggcc ggcggcctcg aaatcggcgg gccgttcgcc
     3961 gacgacccag accgagcgtg cggcccgggg caggtagatg ccgtggcggc gatacaggta
     4021 ggtgaacgcg cggcccgccg gcagccccgc cccggccagc cggcggagat ccgacgcgga
     4081 acgtcgatcc ggtccggcgg gaatcggcaa gaaatgcgcg agcacctgtc gcgcgatggc
     4141 cgtccacgag aggcattcct cggcgaccgc gcgcgcggcg agccccatgc tccgccgctc
     4201 cgcgggctcg cgcagcaggc ggcggatggc ctccgcggcg gcgcgcgcgt cctccgacgg
     4261 cacgcggatc accgcggccc gctgctcgag gatttccacg ccttcgacgg aggccgcgac
     4321 ggccggggcg ccgcacgcgg cgtactcggc gagtttcata ggggtgccga gcatccggcc
     4381 gcgcgcctgc gtgcgcagcg cgccgacgcc gacctcgaag cagttgatcc accacggcat
     4441 atcctcgtgc ggcacatggc ccttgaagag cacgcggtcg gacaagcccg cttgcgcggc
     4501 ctgccggcgc aggttgtcgg cctgggggcc ctcgcccagg atcacgacgc ggacgtcgcc
     4561 cggcaaatgc cgaaggctct cgatcagcac gtccacgcct tgccagcggt tgaggctgcc
     4621 cgcgtagccg acatagcggg cggacgggtc gagccctgcg cgcgcgaggg actcgtcgcg
     4681 gggcagcggg cggaaccggt cgaggtcggc gcccatcccg gccacgaaca cgcggccggc
     4741 ttcgatgcct tgctccacga ggatattttt cagatggcgc gtcgaggtcc ggatccggtg
     4801 cgcgctgcgc gcggccagaa gctggttccg gcgggacagc gcgttgaccg ccgtcgagta
     4861 gcccgacacc tccaggtagg cgctggccca ggtgttatgc tcgccgacca cgacggcgcc
     4921 gcgggcgcac agccgggccg ccgcgcagga cagcggcgag caggaccgga agcggacgta
     4981 gatcgcgtcc ggccgttcgc gccggatggc gcgggccagg acgcccaggt gcgcggccgc
     5041 ccgcaggagc tgcccggcat acccctcgtt gaatcggggc gtggcgtata cgcgcgcgaa
     5101 gggggcggga tcgcgcgccc ggtcgcgccg ggcggggatc accccgatca cctcgtggcc
     5161 ttccgccgcg aaggcccggg ccagggaccc aaaatggatg cggcctgcgc tgggcgtttc
     5221 ccagtcgagc aggcatgtca taaggatctt caaccgcgat ctccgggtat tcgcgccgcc
     5281 gtcgcccgcc aatattcccc atcggggccg cgggggcaag ccgggcgccc gcctatatgc
     5341 gctttgccag gggatgcggc catggtatgg gaacggcggc atggacctga acgtggcatg
     5401 cgtgaaatgg gggacgaagt tcccggcgga ctatgtaaac cggctgcgcg ccatggtccg
     5461 gcggaacctc cctctgccgc atcgcttcgt gtgcctgacg gacgatccgg cggggctggc
     5521 gtcggggatc gagcccctgg ggctgcgggg cggcctggag tactgctgga acaagctgga
     5581 actattccgg ccgggcctgt tttccggtga cgacatctgc ctgtatttcg atctcgacgt
     5641 cgtgatcacc gggccgattg acgcgctgct atccttccgg ccgcaggatg tcttcgtggg
     5701 gctgtacgac tggtaccggt cgcgcgtgcc gcactacaac tcttcggtca tgcgcttcca
     5761 gggcgggcgt cacgagaccc tgtacacgtc ccttggagag aaactggcgg cgggccgggt
     5821 ccggtgggcg cgggaatacg acgcgtatct cggctcgaac gacaaggtcg tgttgtggga
     5881 ggacgagacc cgctacggag gcgaccagga gtggatctca cgatacgtct acgcgccgcg
     5941 ggaactgaag gagcacagct tcccgggagg atggctgctg agctaccgga agcatggacg
     6001 cagggaactc ccccgcgggt gcaaggtcat ggtctttcac ggcgacccca agccccacga
     6061 agtcgagaac gactacgtcc gcgaacactg gcggtgagag ccgcccgctc ttttcgcggc
     6121 cgctcatgct ttgtgatgcg aagactcctt ctcccgcatc tgaaacgagg agcgtcgcgg
     6181 ccgcgggtaa ataacctgtg tatcacagtt tataaactgt gatatgtaga gcctcgaaat
     6241 tcgcggaatc cttaagaata tgacgcgtct tgcccgtgta tcacagttta taaactgtga
     6301 tatattcaat ggtcatacac ttcggtcttt aaccgccggc tttcctgcga accgggcaaa
     6361 cgctgtaatc gcttcggcca gccgctcggc gatgcgggat cggacttctt cctgcgacag
     6421 gaatggggtc tgccggcttg cgttcagaag gctgtccgcg cagttctcgg cccggggctg
     6481 ccgttccaaa agggaagaaa cgcgcgtccg cgcggcccga cgggacaggc cgaggtcgtc
     6541 ggccagtcgc cgcagcccgc cccgcaaccg ggtccacccc cacgggatcc ggcgctgcac
     6601 ccggcacggc cgatactcca atgccagcgt gtccagccaa tcctcccact tgaaggccag
     6661 gatgtcgtcc gaactggcga gcggcaccca cggcacgccg aaggcgtcgg ccaccacggc
     6721 gccgtgcagg gactccgcca ggacgagggg cgccgcgcga accgcgtcga tgaaggcttc
     6781 cgccggcgtc cgaggatcga ccagacgaac gcctgccagt gcgcagggcg tcgcccaatc
     6841 acagaccagc gcggaatcga tatgcgggat gaatacgggc gctcgcgcct tggcggggcc
     6901 cgcggcagac atcaggcggg gcaacagcat ggccggatcg ctgatcgcct tggagtcctc
     6961 gacccccaac cgggcggccg tgcgcggacc gcggacaaac cagacccggt accgggggtc
     7021 cggagcacgg tctccatatc cgcaaccgga gccgaggaca tgtatgcaac gggtcgcggc
     7081 gggcaccggc atcccggagc acaaaatagt gccgatcccg aacaccactt cgcccggatc
     7141 gccgtccaac acgcccggca tgcag
//
LOCUS       DAOOTK010000143         7096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6575_length_7096_cov_4.629314, whole genome shotgun sequence.
ACCESSION   DAOOTK010000143 DAOOTK010000000
VERSION     DAOOTK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6575_length_7096_cov_4.629314"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PK634_12510"
     CDS             <1..1075
                     /locus_tag="PK634_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOW98600.1"
                     /translation="SVAVGGTHGKTTTTAMIAQALRHAGLDPGFCIGGEVDELGGVAG
                     VGDGRLLVVEADESDGTLALYRPDIALVTNIELDHLEHFDGEAGLIACFGKFVRGTRR
                     KVIYCADDPRASSLCRSVPNALSYGWAPGAEVRGLLRASSPAHAEVEVRIKGRRAGVF
                     RLPVAGPQNAQNALAACAAAIEAGLTFEQAAAGLAEFRPAKRRFEIVAEMRGVTVVSD
                     YAHHPTEIRAMMRVAAGRPARRRLAVFQPHRYTRTRALGPEFPPAFAGADEIWLLPVY
                     AASEAPLEGGTSEDLLGHFRAFGGPPVRLLRSLEDAREQWRGWWKDGDLLLVIGAGDV
                     EKIAWWAKDDLIGGNTGEDKKRA"
     gene            1072..1968
                     /locus_tag="PK634_12515"
     CDS             1072..1968
                     /locus_tag="PK634_12515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOW98601.1"
                     /translation="MTKKKFRRVAVLKGGPSAEREVSLRSGAGVAKGLREAGYEVDEI
                     LLEGESVDIPSGVEAVFIALHGRFGEDGGLQEMLRRRGIAYTGSGPEASRRSFDKRLT
                     KQALVREGLPTPAYEVLRDGQARTLPLPVVVKPPCQGSSIGVYRAMTESEWPGALAGA
                     LEHDAEVLVESFVEGRELTVSILDGEALPVLEIRPLEPFFSYTAKYTAGRSEFLVPAP
                     LPEETASRCRNLARRTFRALGAEGFGRVDFRLAADGGLFILELNTIPGFTETSLFPKA
                     ARAAGMTFPEVCDRIMSRASVH"
     gene            2071..2892
                     /locus_tag="PK634_12520"
     CDS             2071..2892
                     /locus_tag="PK634_12520"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOW98602.1"
                     /translation="MRREVRLRETILQVEKPRRARQRERLYQVGSIVLLLVVAAAALW
                     LGTEGLRRAGRALFSGNDRFIVRNLDLASDGKLQPSHIREYAGLDTGLNLFALDLRRI
                     RRELESVPVVGMVTVTRILPDTLRIRISERMAVARLGDEAGGQALAVDREGYTLGPSS
                     VSTRLPVITGYRARGMRPGHRVEDDGIQDALALIELSDEPVYSRFVRIRRVDVSDNEM
                     LRVDLERGERIRFPRREMASRMERLCEIIRQSADEGRAIASVDMMVDRNFPVVFQ"
     gene            2909..4117
                     /gene="ftsA"
                     /locus_tag="PK634_12525"
     CDS             2909..4117
                     /gene="ftsA"
                     /locus_tag="PK634_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOW98603.1"
                     /translation="MSNEPPVVAVEMGTSRIRALVGEVRDGGQCMITGLGECPSRGVR
                     KGEIVHVETAETCLRSALQQAEENGHVHIHQVHLVVTGGHIDSLVNRGSVPVMDPDHE
                     VLEEDVEHAMEHARSVGLSADRRVLHTICQHYYVDDQEGVPDPVGMEGAKLSVDMLVI
                     HGLLNRMRNLVRVVLGAQVEVEDVAFSGLCAALAVLTREDKENGVVMVDLGGGTTDFV
                     AYARGVIADAGCFAVGGDHVSNDIARGLHLSVAEAEHLKETEGGVLVDLAARSQKVVL
                     GGEAGQAARAVKLADLNTIIHARMDETLRLVRDRMEAKGLLHALGGGVVLTGGGARTK
                     DLLPLAEKVFGLPSRIGCPVGLAGLAVVAESPQFAAPAGMVKYAFRTRPPEKGLGSAI
                     KKLFQSWFGS"
     gene            4114..5295
                     /locus_tag="PK634_12530"
     CDS             4114..5295
                     /locus_tag="PK634_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOW98604.1"
                     /translation="MSGLQPTRLNGGEERAAACRVLVLGVGGGGCNSVARMAAGWTSG
                     PETRLVNTDAQALAACGMPDRQLLVGCSLTRGMGTGGDPNLGRLALEEDADLVREALV
                     GVDVLLLVVTFGGGTGSGAAPAIARLAREAGVTTIGLATLPFEFEGLRRRQLAAEGLD
                     ALRLHADMVVTIPNQHLLDLAGDQESLQDAFARTDAMLGVGVRSLWKLLTQPGIINLT
                     LADLRNMVEHSSGSCTFGYAQGSGSKKAEQALARLMKSPLLGEGRLLASAQALLVNIV
                     GGPDLALAEVTRVMNELTGRAQPDVRFFPGAAIEEDWKGKMAIVVLAAEAWDESPLSP
                     RSAPSPDEILPGAAPVQPELPFSEEERGRFRGVDPTVVGGQDLDIPTYLRRGIRLSFE
                     R"
     gene            complement(5326..5616)
                     /locus_tag="PK634_12535"
     CDS             complement(5326..5616)
                     /locus_tag="PK634_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98605.1"
                     /translation="MSEISDIYNSARRNAPVAPAPDPDAPPPVSTPDDLSTGHGASLK
                     RAVVLGLLMILLLAAIAYIEALTDRKDRVRPSETDELELFQRDLDLDAEATL"
     gene            complement(5680..6030)
                     /locus_tag="PK634_12540"
     CDS             complement(5680..6030)
                     /locus_tag="PK634_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98606.1"
                     /translation="MKKLLCVFAMAFAAIPFVGCEDYDEKANRLTFENASHFSVQVIS
                     LTTEWNGFNLAPGERVRMNDIRDVDYRYEPEGSVQEGSASSDRYIVFVDAPPSDDEED
                     AATDEATTDEAAAQ"
     gene            complement(6120..6195)
                     /locus_tag="PK634_12545"
     tRNA            complement(6120..6195)
                     /locus_tag="PK634_12545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6160..6162),aa:Lys,seq:ttt)
     gene            6341..7090
                     /locus_tag="PK634_12550"
     CDS             6341..7090
                     /locus_tag="PK634_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOW98607.1"
                     /translation="MARILTDEFIERRHRFPQRRFQPVHALLAVEVLVALNLFVAGIL
                     LFGSQGPGLLAIGALAGFACVGHAVWIQKRENERRAIDARWHEGVGLTERLKAALGED
                     CYILEDVGMPGGWRRVPVRLLAITPRGLFVIEVRFWRGLVEGAGQDAWWTLRPAPGSK
                     PFRVPNPLVELRRQVEAVDRHFQRGGVEWPDIQPVLVMDAPGTEWRIEEASTPILDPE
                     AAARHVATWSAGRAYSIRKIDQATGRLREAL"
BASE COUNT         1083 a         2264 c         2645 g         1104 t
ORIGIN      
        1 ttccgtggcc gtcggcggaa cccacggtaa gacgacaacc acggccatga tcgcgcaggc
       61 gctacggcac gccggcctgg atcccggctt ttgcattggc ggggaggtgg acgaactggg
      121 cggagtggcc ggggtggggg acggacgctt gcttgtcgtc gaggccgacg agagcgacgg
      181 cacgctggcc ctgtaccgcc cggatatcgc gttggtcacg aacatcgaat tggaccacct
      241 ggagcatttc gacggggagg ccgggctgat cgcgtgtttc gggaagttcg tccgggggac
      301 gcgccgcaag gtgatctact gcgccgacga tccgcgggcc tcgtcgctgt gccggtccgt
      361 tccgaacgcc ctttcctacg gctgggctcc gggcgcggag gtgcggggcc tgctgcgcgc
      421 gtcctccccg gcgcacgcgg aagtcgaggt gcgcatcaag ggccgacgcg ccggcgtctt
      481 ccggcttccg gtggcgggcc cgcagaacgc ccagaacgcc ctggcggcgt gcgccgcggc
      541 catcgaggcg gggttgacct tcgagcaggc cgccgccggg ctggcggaat tccggcccgc
      601 gaagcggcgt tttgagatcg tcgccgaaat gcgcggggtc accgtggttt ccgactacgc
      661 tcatcatccc acggaaatcc gggcgatgat gcgcgtggcg gccgggcggc ccgcgcggcg
      721 tcggctcgcc gtgttccagc cgcaccggta tacgcgcacg cgggcgctgg gtcccgagtt
      781 cccgcccgcc ttcgcgggcg cggacgagat atggctcctg cccgtctatg cggcgtccga
      841 ggcgccgctt gagggcggga catccgagga cttgctgggc cacttccggg ctttcggcgg
      901 gccgcccgtc cggctgctac gctccctgga ggacgcccgg gagcagtggc ggggttggtg
      961 gaaggatggc gacctgctgc tggtgatcgg cgcgggcgac gtggaaaaaa ttgcatggtg
     1021 ggcgaaggat gatttaatag gcggtaacac gggagaggat aagaaacgcg catgacaaag
     1081 aagaagttca ggcgggtggc cgtgctgaag ggcggcccgt cggccgagcg ggaggtgtcc
     1141 ctgcgctccg gcgccggcgt cgccaagggg ctccgcgagg cgggctacga ggtcgacgag
     1201 atcctgctcg agggcgagag cgtcgacatc ccgtccggcg tcgaggcggt gttcatcgcc
     1261 ctgcacgggc ggttcggcga ggacggcggc ctgcaggaga tgctgcggcg tcgcggcatc
     1321 gcgtacacgg gctcgggccc cgaggccagc cggcgctcgt tcgacaagcg cctcaccaag
     1381 caggccctcg tgcgcgaggg gctcccgacg ccggcgtacg aggtgttgcg cgacggccag
     1441 gcccgcaccc taccgctccc ggtcgtggtc aagccgccct gccagggctc gagcatcggc
     1501 gtgtaccgcg cgatgaccga gtcagagtgg ccgggggcgc tggcgggcgc cctggagcac
     1561 gatgccgagg tgctggtgga gtccttcgtc gagggccgcg agctgacggt gagcatcctg
     1621 gacggcgagg cgctgccggt gctggagatc cggccgctcg agcccttctt cagctacacg
     1681 gccaagtaca cggcgggccg cagcgaattc ctggtcccgg cgccgctccc cgaggagacg
     1741 gcaagccggt gccggaacct ggcccgccgc acgtttcgcg cgctgggcgc cgagggattc
     1801 ggccgcgtgg atttccggct ggcggcggac ggcgggctgt tcatcctgga gctcaacacg
     1861 atcccgggct tcacggagac cagcctcttt cccaaggcgg cgcgcgcggc cgggatgacg
     1921 tttccggaag tttgtgatcg aatcatgagc cgggcatcgg tacattgaat ccgtgcccgt
     1981 gtaacagggc gccggcgcga atccggcggc ccggggtgat gaaccgtgtg gtactggcaa
     2041 cgatatcgaa atgaccggcg gcgcgcgggc gtgcgccgcg aagtccggct gcgcgagacg
     2101 atcctccagg tcgagaagcc gcggcgagcg cggcagcgcg aacggctcta ccaggtcggc
     2161 tccatcgtgc tgctgctggt ggtcgcggcc gcggcgctct ggctgggaac ggaagggctg
     2221 cgccgggccg gccgcgcgct gttctccggc aacgaccggt tcatcgtgcg caacctcgac
     2281 ctcgcctccg acggcaagct gcagccgtcg catatccggg agtacgcggg gctggacacg
     2341 ggcctgaacc tcttcgccct cgacctgcgc cggatccgcc gcgaactgga aagcgttccg
     2401 gtcgtcggca tggtcacggt gacgcgcatc ctgccggaca ccctccggat ccggatctcc
     2461 gagcggatgg ccgtggcccg gctcggggac gaggcggggg gccaggcgct ggccgtcgac
     2521 cgggagggct ataccctggg ccccagttcc gtgtccacgc ggctcccggt gatcacgggc
     2581 taccgcgccc gcggcatgcg gcccggccac cgggtggagg acgacgggat ccaggacgcg
     2641 ctggcgctga tcgagttgag cgatgagccg gtgtacagcc gcttcgtccg catccgccgg
     2701 gtggacgtgt ccgataacga gatgctgcgc gtggacctgg agcgcgggga gcggatccgc
     2761 ttcccgcgcc gcgagatggc gtcccgcatg gagcggttgt gcgagatcat ccggcagtcg
     2821 gcggacgagg gccgcgccat cgcctcggtg gacatgatgg tggaccgcaa cttccccgtc
     2881 gtcttccaat gagaaggcgc gacccggcat gagcaacgaa ccgcccgtgg tggccgtcga
     2941 gatgggaacc agccggatcc gcgccctggt gggcgaggtc cgcgacggcg ggcagtgcat
     3001 gatcaccggg ctcggcgagt gcccgtcccg gggcgtgcgc aagggcgaga tcgttcacgt
     3061 cgagacggca gagacctgcc tgcgcagcgc ccttcagcag gccgaggaaa acgggcacgt
     3121 ccacattcac caggtccacc tcgtcgtcac cggcggccac atcgacagcc tcgtcaaccg
     3181 cggcagcgtg ccggtgatgg atcccgacca cgaggtcctg gaggaggacg tcgagcacgc
     3241 catggagcat gcccgttcgg tcggcttgag cgccgaccgc cgggtcctgc acacgatctg
     3301 ccagcattac tacgtcgacg accaggaggg cgtcccggac ccggtcggca tggaaggcgc
     3361 caagctgtcc gtggacatgc tggtgatcca cggcctgctc aaccgcatgc gcaacctggt
     3421 ccgggtcgtg ctcggcgcgc aggtcgaggt cgaggacgtc gccttctcgg gcctctgcgc
     3481 ggcgctggcc gtgctgacgc gcgaggacaa ggaaaacggc gtggtcatgg tggacctcgg
     3541 cggcgggacg accgacttcg tggcgtacgc ccgcggcgtc atcgcggacg ccggctgttt
     3601 cgcggtgggg ggcgaccacg tcagcaacga tatcgcgcgg gggcttcacc tctccgtggc
     3661 cgaggcggag cacctgaagg agaccgaggg cggggtcctc gtggacctgg ccgcgcgctc
     3721 ccagaaagtc gtgcttgggg gagaggccgg ccaggcggcc cgggccgtca agctggcgga
     3781 cctgaacacg atcatccatg ctcggatgga cgagacgttg cggctggtgc gcgaccggat
     3841 ggaggcgaag ggattgctgc acgcgctggg cggcggcgtg gtgctgaccg gcggcggcgc
     3901 gcggacgaag gacctgctgc ccctggcgga gaaagtcttc ggcttgccaa gccggatcgg
     3961 gtgtcccgtc gggctggccg gcctggccgt ggtcgcggag agcccgcagt tcgccgcgcc
     4021 cgcgggcatg gtcaagtatg cgttcaggac ccgccccccc gagaaggggc tgggcagcgc
     4081 gatcaagaaa ctctttcaat cgtggttcgg atcatgagcg gactccaacc gacgcggtta
     4141 aacggcgggg aagagcgcgc ggcggcctgc cgggtgctgg tcctcggggt gggcggcggg
     4201 ggctgcaaca gcgtggcccg gatggccgcc ggctggacgt cggggccgga gacgcggctt
     4261 gtcaatacgg acgcgcaggc gctcgcggcg tgcgggatgc cggaccggca actcttggtg
     4321 ggctgctcgc tgacccgcgg aatgggcacg ggcggcgatc ccaacctggg ccgcctggcc
     4381 ctcgaggagg acgcggacct ggtccgggag gccctggtcg gcgtggacgt gctgctgctg
     4441 gtcgtgacgt tcggcggagg gaccggcagc ggcgcggccc cggccatcgc gcgactcgcg
     4501 cgcgaggcgg gcgtcacgac gatcgggctg gccacgctgc cgttcgagtt cgagggcctg
     4561 cgccgccgcc agctggctgc ggaggggctc gatgcgctcc ggctgcacgc ggacatggtg
     4621 gtcacgattc caaaccagca cctgctggac ctggcgggcg accaggagag cctgcaggac
     4681 gccttcgcgc gcacggacgc gatgctcggg gtcggcgtgc gctcgttgtg gaaactgctg
     4741 acccagcccg ggatcatcaa cctgacgctg gccgatctgc gcaacatggt cgagcacagc
     4801 agcggttcct gcaccttcgg ctatgcccag ggctcgggct cgaagaaagc cgagcaggcc
     4861 ctggcccggc tgatgaagag cccgctgctc ggcgaaggac gccttctcgc ttccgcgcag
     4921 gccctgctgg tgaacattgt cggcggcccg gacctcgcgc tggccgaggt cacgagggtc
     4981 atgaacgagc tcaccggccg ggcccaaccc gatgtgcgtt ttttccccgg cgcggccatc
     5041 gaagaggatt ggaagggaaa gatggccatc gtggtcctgg cggcggaggc ctgggacgaa
     5101 agcccgctct cgccgcggag tgcgccgtcc cccgacgaga tcctgccggg cgcggccccg
     5161 gttcaacccg aacttccgtt cagcgaagag gagcggggcc ggttccgggg cgtggatccc
     5221 acggtcgtgg gagggcagga tctcgatatc cccacgtatc tccgccgcgg catccggctc
     5281 tcgttcgagc gctgacggcc ctattgcgcc cgcggatgga tgcgatcaca acgtggcttc
     5341 cgcgtcgagg tctaggtccc tctgaaacag ttccagttcg tccgtctcgc tcggacgcac
     5401 ccgatccttg cggtcggtca gagcctcgat gtaggcaatc gcggccagga gcaggatcat
     5461 gaggagcccg agcacaacgg ctctcttcag gctggcgccg tgccccgtgg acaagtcatc
     5521 gggagtggaa accgggggcg gcgcatccgg atccggggcg ggcgcaaccg gagcgtttct
     5581 ccgcgcggag ttgtagatgt cgctgatttc agacatggcc gcgcgggtcc gtccgacggg
     5641 cggtgaagat ggatacgaat ccgcgcatag cgaccggcgc tactgggcgg cggcttcgtc
     5701 ggtggtggcc tcgtcggtgg cggcgtcttc ttcgtcgtcg gacggcgggg cgtccacgaa
     5761 gacaatgtac cgatcgctgg aggcgctacc ctcctgcacc gagccctccg gctcgtaccg
     5821 gtaatccacg tcgcggatgt cattcatccg gacccgctcg ccgggcgcga ggttgaatcc
     5881 gttccactcg gtggtgagcg agatgacctg gaccgaaaag tgagacgcgt tttcgaacgt
     5941 cagccggttg gccttttcgt cgtaatcctc gcacccgacg aaggggatcg ccgcgaaggc
     6001 catcgcaaag acgcagagaa gctttttcat gggctctatc ctctattatg acacggctta
     6061 acgattgatg gtggattacc atgccagaag gaatgcagta gtcaagagcg atgatggggt
     6121 ggtgagccgg gcggggctcg aacccgcgac cacaagctta aaaggctcgt gctctaccaa
     6181 ctgagctacc ggcccccccg tgaaaaacga cgcggaaaat agggcaatcg cttccgggtg
     6241 tcaaaatatc atttgcccgc cggaagtcca gagacccgtg attgacccgc gtcgggcggc
     6301 cgtgtaaaaa ggcggcggtg gcgggacgtg aggggcggat atggcgcgta tcctgaccga
     6361 cgaattcatt gagcggcggc accggtttcc ccaacggcgg ttccagcccg ttcatgccct
     6421 gctggctgtg gaagtcctgg tggcgctcaa tctcttcgtg gccggcattc tcctgttcgg
     6481 atcccagggg ccgggcctgc tggctatcgg ggccctcgcc ggctttgcct gtgtcgggca
     6541 tgccgtgtgg attcaaaagc gggaaaacga gaggcgcgcc atcgacgcgc ggtggcacga
     6601 aggcgtcgga ctcaccgagc ggctgaaggc cgccctcggc gaggattgtt acatcctgga
     6661 ggacgtcggg atgccgggcg gctggcggcg cgtgccggtg cgcctgctgg cgatcacgcc
     6721 gcgcggcctc ttcgtgatcg aggtgcgctt ctggcggggc ttggtggaag gcgccgggca
     6781 ggacgcgtgg tggaccctgc gcccggcccc gggaagcaag ccgttccgcg tgcccaaccc
     6841 gctggttgaa ctccggcgac aggtcgaggc ggtggaccgg cacttccagc ggggcggcgt
     6901 cgaatggccc gacattcagc ctgtcctggt catggatgcg ccgggcacgg aatggcggat
     6961 cgaggaggcg tccacgccca tcctggatcc ggaggcggcg gcgcggcacg tagcgacgtg
     7021 gtcagccggg agagcctact cgatccggaa gatcgaccag gcgaccgggc ggctgcgcga
     7081 agccctgtaa cggtgt
//
LOCUS       DAOOTK010000144         7072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6600_length_7072_cov_4.764144, whole genome shotgun sequence.
ACCESSION   DAOOTK010000144 DAOOTK010000000
VERSION     DAOOTK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6600_length_7072_cov_4.764144"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3579
                     /locus_tag="PK634_12555"
     CDS             <1..3579
                     /locus_tag="PK634_12555"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW98608.1"
                     /translation="NPNLRAVYTDVRFGCSAPRPDGEGNIDDDPQLLALLNPHLAEAS
                     PCRGAGTTNGVEEGETDIDDEPRVVSGQTDVGCDQYTGGQIAGALTVDILAPYDALLT
                     GVPLAMSADVIGKAYWQRWLIQTNSGARMVSNAVQVTQAWPVAGVYTVLLEAANATLT
                     NADTLVVYVSESAEHYASPDGANEYPYTNWKTAANSLLDAVETCPAGGILYVGTGVYF
                     EADTVRVTKPLSILGVGPAEEIQVDGQGAIPCFYLNHASAVLSNLTLVNGWSPDQGGG
                     AYVRDGLVTHCHVISNYAFEQGGGLMAWGDARVEWCTIAGNEAGQWGGGIMANERAVI
                     RNCFLQDNAAHNYGGGADISDGVRLQNCFITGNRAFGGGGVHLDGGGIAQNCTILKNE
                     ATDSGGGILHRFADTGATYNCIVYHNESPVGPNIAFQVSTTGTVEHTCSSPLFGGAGN
                     ITNEPALLGLDNPHIVASSPCIGAGSTNYIEGIEEDIDGEARVFGGAVDMGCDEFIGT
                     NILGALTAAIRGETQVVAGEAAELFADIRGKAWGYVWNVAESGATNVIPDTFEIAPAW
                     AAAGWYDVILAASNLETTAAATVSVHVIEGGFTNYVSPDGGHVAPFTNWTDAATNVQA
                     AIDACWVGGVVMVGTGTYAEGRTIELNKPVAVVGAGAPEDCVFDGEMAYRVINLDDPR
                     ALLANIGVTGGWDEDAAGVAMLEGTVSNCVIAGNINAVVGGGIRCAGHSLLTDSLVTG
                     NISQNWPGGVFCSGYGVVRNCLIEGNLAQVHGGGVYLDQGGVVTGCVIEANSAMSGGG
                     AYLHYAGSVTHSTIHSNDSANGAGAFLYWGGVVTNCDITWNWGEMDGGGLWFNGGGLV
                     TDSRVQRNFADEGAGSGGGAVLSQGGVLRRCDVSTNTASAGAGIYSQYGGLIDRCHIH
                     HNVAPEGWGQASGGGVKLYQGDEIRNGLIEHNSSSNVAGGLLMLVGGTARNCTIANNT
                     AGGEAGGMAVGSAGTLYNCIVYANTAPDAPNWDVDPGAVVDHCCTTPDFPAFVGTIAA
                     DPAFEGGGSYRLRTNSPCVNAGVNDYWMNGATDLDGSARIQNVAVDVGAYESAWWGLF
                     TDVDEDDFNDWVEVNVTGTDPADETSFLGMRDFGVQGPGAEGIVVRWQSVAGKLYHVD
                     RATNLVESPKFTNWMTGVVGLPGATEVTDTTATAGAPWFYRVGIEP"
     gene            complement(3584..5377)
                     /locus_tag="PK634_12560"
                     /pseudo
     CDS             complement(3584..5377)
                     /locus_tag="PK634_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
     gene            complement(5488..>7072)
                     /locus_tag="PK634_12565"
     CDS             complement(5488..>7072)
                     /locus_tag="PK634_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOW98609.1"
                     /translation="NDICGQTFAGLWLPTPPGLLEVLRNRVCDNHPYGVLIDQARQLR
                     FASNLILGNSTGLWLWQGSAWNSPLNAAVYNNAVVGNRDYGVRLTAWRSGAGVQVLNN
                     IVAFNAGKGLLVQGRPFQGVLAYNDFFQNAGGAVAGLAADPIGRQGNIAADPRFLDHN
                     GALSGNYHLAPGSPCLHAGSPAPAFRNADGSRNTMGLYGGPERRPALGWATTWKGLAL
                     LYVDHGRGDVLLCHTNARGSICRANPFNGGAWVVANHYRPLEGSSIVAVDSQGRELQR
                     FPGFTECLDIWQSVELSVDPRDGSCWVADTRDAFRHGGPGRLAKLTLDGRIAVDLEIP
                     GLTDVEVARPDGLVAASAGESHAFVHFFTPDGTPVASADAGEGMAIGVAVNPPGGNLL
                     LACDSHWAWLSLDDPGVVLQQAPARLGPRTSSVTVDPNSGRILMTAFAEEDTQLSLFA
                     PDGVVLRNSNLQGRNCSPSLTSACDVDPRDSFFIGSCYLWGEEERRWLEGIAFLDPEL
                     VEPVSRLDGPCSSVSLLRP"
BASE COUNT         1118 a         2349 c         2534 g         1071 t
ORIGIN      
        1 aacccgaacc tgcgcgcggt ctacacggac gtccggttcg gctgctccgc gccgcggccc
       61 gacggcgagg gcaacatcga cgacgacccg cagttgctgg ccctgctgaa cccgcacctc
      121 gccgaggcct ccccgtgccg cggcgcgggc acgaccaacg gcgtcgagga gggcgagacg
      181 gacattgacg atgagccccg ggtggtctcc ggccagacgg acgtcgggtg cgaccagtac
      241 acggggggcc agatcgccgg cgcgctgacg gtggacatcc tcgcgccgta cgatgcgctg
      301 ctgacgggcg tgccgctggc gatgagcgcg gacgtcatcg gcaaggccta ctggcagcgg
      361 tggctcatcc agaccaacag cggcgcgcgg atggtctcca acgccgtgca ggtcacgcag
      421 gcctggcccg tggcgggagt gtacacggtc ctcctggagg ccgccaacgc gacgctgacc
      481 aacgccgaca ccctggtggt gtatgtgtcg gagtcggcgg agcactacgc ctcgcctgac
      541 ggcgcgaacg agtacccgta caccaactgg aagacggcgg cgaacagcct cctcgacgcc
      601 gtcgagacct gcccggcggg cgggattttg tacgtgggca cgggcgtgta tttcgaggcg
      661 gataccgtgc gcgtcaccaa gccgctctcc atcctcggcg tgggcccggc ggaggagatc
      721 caggtggatg ggcagggcgc gatcccgtgc ttctacctca accacgcgtc ggcggtgctg
      781 tccaacctga cgctggtcaa cggctggtcg ccggaccagg gcggcggcgc atacgtgcgc
      841 gacggcctgg tcacgcactg ccacgtgatt tcgaactacg cgttcgagca gggcggggga
      901 ctgatggcgt ggggcgacgc gcgcgtggaa tggtgcacga tcgccggcaa cgaggcaggc
      961 cagtggggcg gcggcatcat ggccaacgag cgggcggtca tccgaaactg cttcctccag
     1021 gacaatgccg cccacaacta cggcggcggc gcagatatct ccgacggggt ccggttgcag
     1081 aactgtttca tcaccggcaa ccgggcgttc ggcgggggcg gcgtgcacct cgacggcggg
     1141 ggcatcgcgc agaattgcac gatcctcaaa aacgaggcca cggacagcgg cggcggcatc
     1201 ctgcatcgct tcgccgacac cggcgcgacg tacaattgca tcgtgtacca caacgagtcg
     1261 ccggtcggcc cgaacatcgc gttccaggtc tccacgaccg ggacggtgga gcacacctgc
     1321 tcctcgcccc tgttcggcgg cgcggggaac atcaccaacg agccggccct gctgggcctc
     1381 gacaacccgc atatcgtcgc gtcctcgccc tgcatcggcg cggggagcac caactacatc
     1441 gaagggatcg aggaggatat agacggggag gcgcgggtct tcggcggcgc ggtggacatg
     1501 ggctgcgacg agttcatcgg gacgaacatc ctgggcgcgc tgaccgcggc gatccggggc
     1561 gagacgcagg tcgtggcggg cgaggccgcc gaactgttcg cggacatccg cggcaaggcg
     1621 tggggctacg tctggaacgt cgcggagtcc ggcgcgacca acgtcatccc ggatacgttc
     1681 gagatcgcgc cggcgtgggc ggcggcgggc tggtacgacg tgatcctcgc ggcctccaac
     1741 ctcgagacca cggccgccgc caccgtgagc gtgcacgtga tcgagggcgg gttcacgaac
     1801 tatgtctctc cggacggcgg gcacgtcgcg ccgttcacga actggaccga tgccgcgacg
     1861 aacgtccagg cggcgattga cgcctgctgg gtggggggcg tggtgatggt gggtaccggg
     1921 acctacgcgg agggccgcac gatcgagctg aacaagcccg tcgccgtggt cggcgccggc
     1981 gcgccggagg actgcgtgtt cgacggcgaa atggcgtacc gcgtgatcaa cctggacgat
     2041 ccgcgcgccc tgctggcgaa tatcggcgtg acgggcgggt gggacgagga cgcggcgggc
     2101 gttgccatgc tcgaggggac ggtcagcaac tgcgtcatcg cgggcaacat caatgccgtg
     2161 gtgggcggcg gcatccgctg cgccgggcac agcctgctca cggacagcct ggtcacgggc
     2221 aatatctccc agaactggcc cggcggcgtg ttctgctccg gctacggcgt cgtgcggaac
     2281 tgcctgatcg agggcaacct cgcgcaggtc cacggcgggg gcgtgtacct cgaccagggc
     2341 ggcgtggtga ccggctgcgt catcgaggcc aactcggcca tgagcggggg cggcgcgtac
     2401 ctgcattacg cgggctccgt cacgcactcg acgatccaca gcaacgattc ggccaacggg
     2461 gcgggcgcct ttctctactg gggcggggtc gtgaccaact gcgacatcac gtggaactgg
     2521 ggcgagatgg acggcggcgg cctctggttc aacggcggcg gcctggtgac cgacagccgg
     2581 gtccagcgca acttcgccga cgagggcgcg ggcagtggcg gcggcgcggt cttgagccag
     2641 ggcggcgtgc tgcgccggtg cgacgtctcc accaataccg cctccgccgg cgcgggcatc
     2701 tactcgcagt acggcgggct gattgatcgt tgccacatcc accacaacgt ggcgccggag
     2761 ggttggggcc aggcttcggg cggcggggtg aagctctacc agggcgacga gatccgcaac
     2821 ggcctgatcg aacacaacag ctcaagcaac gtggccggcg gattgctgat gctggtgggc
     2881 ggaacggcgc gcaactgcac catcgcgaac aacacggcgg gtggcgaggc cgggggtatg
     2941 gccgtcggat ccgccggtac actgtacaac tgcatcgtct atgccaacac cgcgccggac
     3001 gcccccaact gggacgtgga cccgggagcc gtggtggacc attgctgcac cacgcccgac
     3061 tttccggcgt tcgtcggcac gatcgccgcc gacccggcat tcgagggcgg cgggagctac
     3121 cgtctgcgga ccaactcgcc gtgcgtaaac gccggggtga acgactactg gatgaacggg
     3181 gcgacggacc tcgacggcag cgcgcgcatt caaaacgtcg cggtggatgt cggggcgtac
     3241 gagtccgcct ggtgggggct gttcacggac gtggacgagg atgacttcaa cgactgggtt
     3301 gaggtgaatg tcacggggac ggatccggcg gacgagacct cgtttcttgg gatgagggat
     3361 ttcggggttc agggtccggg ggcggaaggg atcgtggtcc ggtggcagag tgtcgcgggc
     3421 aagttgtacc acgtggaccg cgcgacgaac ctggtcgaaa gcccgaagtt cacgaactgg
     3481 atgacgggcg tagtcgggct tccgggcgcc accgaggtga cggatacgac cgccaccgcg
     3541 ggggccccct ggttctaccg cgtcgggatc gagccgtgac ccgtcagccc tggagcttgc
     3601 gttccagggc cgacagctcc tcccgcaggg ccgcgggcag ccgctcgccg agcttcgcgt
     3661 aatgctcgcg gataccgggc aactccgccc gccaggcggc ggggtccacg gagaacaatt
     3721 ccgcctgctc ttcggcgctc atgtccaggc ccgagaaatc cagcgccccg gcggccggga
     3781 gccggccgat cggcgtctcc acggcgcttc cgcccctctc gacgcgctcg aagatccatt
     3841 tcagcacgcg cgcattgtct ccgaagcccg gccacagcca ttggccttcc gccgatttcc
     3901 ggaaccagtt gacgtagtag atgcgcggaa ggtcttccgc gcggctctcg cggccgacct
     3961 tgagccagtg ggcaaagtag tcgcccatgt ggtaaccgca gaacggcagc atggccatcg
     4021 ggtctcgccg gagtacgccg attgtgccga aggcggcggc ggtcatctcg ctgcccatga
     4081 tcgaacccat gaagacgcca tgctgccagt cgcgagcctc cgtcacgagc gggacgaccg
     4141 aggcgcggcg ccctccgaac aggatcgcgg agatcggcac gccggcgggg tcctcccact
     4201 cgggcgcgat caccgggcac tggcgcgccg gcgcggtgaa gcgggcgttg ggatgggcgc
     4261 ccttcttccc ggaggccggg gaccattcct tgccctgcca gtccacgccc tgcgcgggag
     4321 gcgcgcccag gtcttcccac cagaccccgt tgtccggcgt gagcacgcag ttggtgaaga
     4381 tcgtgttgcg ctggatcgtg cgcatggcgt tcgggttgct cttcaggctc gtgccgggcg
     4441 cgacgccgaa gaagccggcc tccggattga tggcgtgcca gcggccgtcc gcggcgcgct
     4501 tcatccagca gatgtcgtcg ccgatggtct ggaccttcca gcccggcagc gtcggctcca
     4561 tcatcgcgaa gttcgtcttg ccgcactggg acgggaacgc cgcggcgacg tagatggatt
     4621 tccccgcggg gttcgtcagc ttgatgatca acatgtgctc cgccagccag ccttcgtcgc
     4681 gcgcctgcac ggaggcgatg cgcagggcgt ggcatttctt gcccagcagc gcgttgccgc
     4741 catacccgga gccgtaggac cagatctccc gcgtgtccgg gaaatgcgtg atgaacttct
     4801 tctggatgtc cgggttgcac ggccaggcga catccttctg cccgggggcc aggggcgcgc
     4861 cgacggagtg catggcccgc acgaactcgc cgtgttcgcc gagcacctcg aggaccttcg
     4921 ttccgacgcg cgtcatgatg tgcatgttga ccacgacgta gggcgaatcg gtgatctcga
     4981 cgccgatatt ggcgatcggg ctgccgatgg ggcccatgct gtagggaatg acgtagagcg
     5041 tgcggccctg catgcagccc tggtacgact tgcgcatcag ggccttcatc tccgccggct
     5101 ccatccagtt gttcgtcggg ccggcgtcct cgcggatcgt cgagcagatg aacgtgcgct
     5161 cctccacgcg ggcgacgtcg ctgggatcgg accggaagag caggcagttc ggccagcgtg
     5221 cgccgtccag ccgggtcgcc gcgccgctcg cgaccatctc gttggacagc cggtcatact
     5281 ccgcctgcga gccgtcgcac cagtgcaccg ctttcggctc gcacagcgcg cgccactctt
     5341 ccacccacgc ccgcaacggc ttgtgcttcg tcatgtcgtc accctcgttc gatcgcgttc
     5401 cttccgcgcc atactcgcag agtgcggcgc agtgtaccgg gccgctcggg agaatgcaag
     5461 cgcttgcgcg ccgcgagcgg gcgtccgtca cgggcggagc agggatacgc tcgagcaggg
     5521 cccatcgagc cgggataccg gctcgacgag ttcggggtcc agaaaggcga tgccctccaa
     5581 ccagcgccgc tcctcctcgc cccataaata gcaggaaccg atgaagaagg aatcgcgggg
     5641 atccacatcg cacgcgctgg tgaggctcgg gctgcagttg cggccctgca ggttgctgtt
     5701 ccgcaggacc acgccgtccg gggcgaacag cgaaagctgc gtgtcttcct ccgcgaaggc
     5761 cgtcatcagg atgcggccgc tgttcggatc caccgtgacg gacgaggtgc gcgggcccag
     5821 gcgggccggg gcctgctgca ggacgacccc ggggtcatcg agggaaagcc aggcccagtg
     5881 cgagtcgcag gccaggagca ggttcccgcc cggcgggttc accgccacgc cgatggccat
     5941 gccttcgccg gcgtccgcgg aggccaccgg cgtcccgtcc ggcgtgaaga aatgcacgaa
     6001 ggcgtgcgat tccccggccg aggcggccac gagaccgtcc ggacgcgcca cctcgacatc
     6061 ggtcaggccc gggatttcca ggtccacggc gatgcgcccg tccagcgtca gcttggcgag
     6121 ccggcccggg cctccgtgcc ggaaggcgtc ccgcgtgtcg gccacccagc aggagccgtc
     6181 gcgggggtcc acggacagct ccacggactg ccagatgtcg aggcattcgg tgaacccggg
     6241 gaaccgctgg agttcgcggc cctggctgtc caccgccaca atgctgctgc cctccagggg
     6301 ccggtaatgg ttggccacca cccacgcgcc gccgttgaag ggattggccc ggcagatgga
     6361 gccgcgcgcg ttggtgtggc acagcaggac gtcgccgcgt ccgtggtcga cgtacaggag
     6421 agcgagcccc ttccacgtgg tggcccagcc cagcgccggc cggcgctcgg ggcccccgta
     6481 caggcccatg gtgttgcggc tgccgtccgc gttgcggaag gccggcgcgg ggctgccggc
     6541 gtgcaggcag ggcgagcccg gcgccaggtg gtagttgccc gagagggccc cgttgtggtc
     6601 caggaagcgg ggatccgcgg cgatgttgcc ctgccggccg atgggatcgg ccgccagccc
     6661 ggcaacggcg ccgcccgcgt tctggaaaaa gtcgttgtag gcgagcacgc cctggaacgg
     6721 ccgcccctga acgagcaggc ccttgccggc gttgaaggcc acgatgttgt tcagcacctg
     6781 gacgccggcg ccgctccgcc atgccgtcag gcggaccccg tagtcgcggt ttccgaccac
     6841 cgcgttgttg tacacggcgg cgttcaacgg gctgttccac gccgatccct gccacagcca
     6901 tagcccggtg ctgttgccca ggatcaggtt gctcgcgaac cggagctgcc gcgcctggtc
     6961 gatcagcacg ccgtagggat ggttatcgca gacccggttg cgcagcacct cgaggaggcc
     7021 ggggggagtc gggagccaca ggccggcgaa ggtctggccg cagatatcgt tg
//
LOCUS       DAOOTK010000145         6941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6749_length_6941_cov_5.364943, whole genome shotgun sequence.
ACCESSION   DAOOTK010000145 DAOOTK010000000
VERSION     DAOOTK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6749_length_6941_cov_5.364943"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PK634_12570"
     CDS             complement(<1..827)
                     /locus_tag="PK634_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOW98610.1"
                     /translation="MAMRKITEGVHWCGVVDWDRRVFDVLVPLPDGTSYNSYLVQGAE
                     KTALLDGVDDAKIPLLLETLRDVPRIDYIVSHHAEQDHSGALPALMAWHPEARLLASA
                     MGRKLLIELLDLPADRVVAVKDGETLALGGKTLRFLYTPWVHWPETMSTWLEEDGILF
                     SCDFYGAHLATSELYAKEGVRVYRAAKLYYAQIMMPYAKQVAKSIEKVSGLGIRCIAP
                     SHGPLYDKPAIVMDSWREWVSAPPQNLAVVAYVSMHGSTRRMAERLVDALIARGVSVR
                     "
     gene            complement(917..3856)
                     /locus_tag="PK634_12575"
     CDS             complement(917..3856)
                     /locus_tag="PK634_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOW98611.1"
                     /translation="MLQPGTTHRGFTVVTTTPLPAPLGRAVELRHGASGARVLHLVND
                     DPENLFCVAFPTPPADDTGLPHILEHSVLGGSHKYPVRDPFFELVKISMATFINAMTF
                     WDRTVYPVASNVKQDLFNLADVYFDAVFHPLLTENIFKREGHHLAPDGAGGLTVSGIV
                     YNEMKGVYSNPEWAMEMIALRSLLPDTIYGRESGGDPRAIPDLTFEQFRNFHSTCYHP
                     ARGLFVCYGNIPTEDYLAFLAERLAGYSARDAAPVPARQPRWPAPRATTETYAIGHDE
                     PASGRTYFRLDWLAGDAAAARDTAALYLLSLLLFGHEAAPLKKALVDSKLGEDVISCG
                     AEPAGLEMTFRVGLKGSEPDRAAAFEKLVLDTLRGLAEEPFAPEAVDAAFHQAGYEYQ
                     EVQSGYPLHVLFRVLQAWTYSDEPLRFLSMRDALAALRAEAAADPLFFSRLIRERLLD
                     NPHRLQLALRPDRGHQARLDAEFAERMKTVRAGLDDAAVQRVAAEAEELDRESGTPNP
                     PEALALLPQLGLHDLPGTPAEIPSNLERVGPGIPLLINDVPSNGVAYLHLDFNLDGLP
                     SEWWPYVSRFTDALTRLGAAGMGYEDVARRQAARFGGMRAWPWFQTRADDPASHLARL
                     RVEIKTLDDRIEPALELLGQLLFGLDPKDLARLQDVVIQARTWERTEKIQGGTTTPLL
                     HAGRGFSLPGHLREVCEGLPQLFLLEELAGRFEQQADLLASRLLSIRDFLLQSRRLAA
                     SFTGPDRSRDAVAAALKDWGSKMADADAGSAAAAFPAFTGPVLEGLAAPLQVAHNALV
                     MPAPHASHPDEPLLALAAHLVSFDYILPEIRFKGNAYGAGCRHDPFLGTLALTSFRDP
                     HVVRTLDVFGKTADFVRQASWSRADLDRAVIGKARDDLKPIRPGEATGLSLHRWLIGM
                     TPERRAERYRRRLRATPEEARRAMLDAIEAGRPRQAICALASREKLEEANRQMPAALS
                     IEDVLKA"
     gene            complement(3861..4253)
                     /locus_tag="PK634_12580"
     CDS             complement(3861..4253)
                     /locus_tag="PK634_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOW98612.1"
                     /translation="MPVFEYECKKCGRVSSFLVRNPSAHKPPACPRCGHPETTRVLSR
                     FASVKSGSAGGEGADLSALDGLDENDPRSLGRAMRKMAEETGEPVPPEMEQMMRRLEA
                     GEDPEKLGAEMEGMEGGGPAGDDTLYEG"
     gene            complement(4451..4849)
                     /gene="rpsI"
                     /locus_tag="PK634_12585"
     CDS             complement(4451..4849)
                     /gene="rpsI"
                     /locus_tag="PK634_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOW98613.1"
                     /translation="MAHKVVEYSATGRRKTSVARVRIAHGVGKTTVNSRDFKDYFPVE
                     AVRQFIEQPFEAADMLKRFDVIANVSGGGIMGQAGALRHGIARALLLADAALREKLKG
                     GGFLTRDPRMKERKKYGQPGARKRFQFSKR"
     gene            complement(4869..5297)
                     /gene="rplM"
                     /locus_tag="PK634_12590"
     CDS             complement(4869..5297)
                     /gene="rplM"
                     /locus_tag="PK634_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOW98614.1"
                     /translation="MKTTLVRESEVVRAWHVVDAAGQPAGRLAVKIANILRGRNKRNY
                     TPHADMGDFVVVINAAQVKLTGSKEEQKIYKDYSGYPSGLKMQKASVIRVRQPTRIVR
                     QAVKGMLPKNHLSRKLITRLKVYAGAEHPHAAQGAKALAV"
     gene            5424..6401
                     /locus_tag="PK634_12595"
     CDS             5424..6401
                     /locus_tag="PK634_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOW98615.1"
                     /translation="MQPLPLIGAAELPACLDRRRRPHQQHYFAMYSSHLGGLVTDPAL
                     MLLPADDHMVHRGDGIFETVKCVGGGLYNLEAHLTRLRLSAGRIRLPVPWTSADLADI
                     VVQTVHAGGRPEALVRILVSRGPGGFGISPAECPEPALYVVAYALPGSFMTLHPGGAR
                     AARSSIPPRAPPFSHLKSVNYLPNVLMKMEALDARVDFTLSFDEKNNLAEGATENAGI
                     VTRGRRLQVPKPDRILPGTTARRVLELARPLIAGGLLSAVEETDIPYAAVREATELLI
                     FGTTPDVTSVIEFDGRPVGDGKPGAAGLALNARLADDILHNAALRTPVF"
     gene            complement(6425..>6941)
                     /locus_tag="PK634_12600"
     CDS             complement(6425..>6941)
                     /locus_tag="PK634_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOW98616.1"
                     /translation="CNDLRLLSSGPNTGLDEIILPPLEPGSSIMPGKFNPSACEAVNM
                     ACLQVFGLDAAVAAACGAGQLELNTYMPLIGFNLLRATRYLERACALLGEKCVRGIQA
                     NREECARHFEHSAGLATVLNPKLGYDRVAELVRESLETGRTLRQLVLEKKIMTEDQFN
                     DVLKHSTGPNL"
BASE COUNT         1065 a         2386 c         2372 g         1118 t
ORIGIN      
        1 cgcaccgaga ccccgcgcgc gatcagcgcg tcgaccaggc gctccgccat ccgcctcgtg
       61 ctgccgtgca tggagacgta cgcgacgacg gccaggttct gcggcggggc ggacacccac
      121 tcgcgccagg aatccatgac gatggcgggc ttgtcgtaga gcggcccgtg gctcggtgcg
      181 atgcagcgga tgcccagccc ggacaccttc tcgatgcttt tcgccacctg cttcgcgtag
      241 ggcatcatga tctgcgcgta gtacagcttc gccgcgcggt acacccgcac gccctccttc
      301 gcgtacagct ccgaggtcgc cagatgcgcg ccgtagaagt cgcatgagaa aaggatgccg
      361 tcctcctcca gccaggtgct catcgtctcc ggccagtgca cccacggcgt gtagagaaag
      421 cgcagcgtct tgccgccgag cgccagcgtc tcgccgtcct tgaccgcgac gacgcggtcc
      481 gcgggcaggt cgagcagctc gatcaggagc ttccggccca tcgcggacgc gagcagcctg
      541 gcttccgggt gccacgccat cagcgcgggc agcgcgccgg aatggtcctg ctcggcgtgg
      601 tgggagacga tgtagtcgat ccgcgggacg tcgcgcagcg tctccagcag cagcgggatc
      661 ttggcgtcgt ccacgccgtc cagcagggcg gtcttctccg cgccctgaac gagatacgag
      721 ttgtagctgg tcccgtccgg caggggcacg aggacgtcga acacccgccg gtcccagtcc
      781 acgacgccgc accagtgcac gccttccgtg atttttcgca tggccataat ggtctccctc
      841 gacgaatcgg gacgaaaagt agcccaaaaa ccggtgaatt gcaaaaaaga tggagggcgg
      901 cgctatgcga cgccgctcag gccttcagca catcctcgat gctcaacgcg gccggcatct
      961 gacgattggc ctcttccagc ttttcgcggc tggcgagcgc gcagatcgcc tgccgaggcc
     1021 ggccggcctc gatcgcgtcc agcatcgcac gccgcgcctc ttcgggcgtc gcgcgcagcc
     1081 ggcggcgata acgctccgcc cgccgctcgg gggtcatgcc gatcagccag cggtgcagcg
     1141 aaagtcccgt ggcctcgccc gggcgaatcg gtttcagatc gtcccgcgcc ttcccgatca
     1201 ccgcgcgatc caggtcggcg cggctccacg aggcctgccg gacgaaatcc gcggtcttcc
     1261 cgaacacgtc gagggtccgt accacatgcg ggtcgcggaa cgaggtcagg gccagcgtcc
     1321 cgaggaacgg gtcgtgccgg cagccggcgc cgtaggcgtt tcccttgaag cggatctccg
     1381 ggaggatgta gtcgaaggag accaggtgcg ccgcgagcgc caacagcggc tcgtccggat
     1441 gggaagcgtg cggagcgggc atgaccagcg cgttgtgcgc gacctggagc ggggcggcca
     1501 ggccctcgag caccgggccc gtgaaggccg ggaaggcggc ggccgcggag cccgcatccg
     1561 cgtccgccat tttgcttccc cagtctttca gggcggcggc caccgcgtcc cgcgaacggt
     1621 ccggccccgt gaaactggcg gccaggcggc gggactggag caggaagtcg cggatggaca
     1681 gaagccgcga cgcgagaagg tctgcctgct gctcgaaccg cccggccaat tcctccagca
     1741 ggaaaagctg cggcaagccc tcgcagacct cgcgcaagtg accgggcagg ctgaatcccc
     1801 ggccggcgtg cagaagcggg gtggtagttc caccctggat tttctctgta cgttcccagg
     1861 tccttgcctg gatgacgaca tcctgcagcc gcgccagatc cttcgggtcg agtccgaaga
     1921 gcagctggcc gagcagttcc agcgcgggct cgatgcggtc gtcgagcgtc ttgatctcga
     1981 cgcgcagccg cgccaggtgc gaggccggat cgtcggcacg ggtctggaac cacggccagg
     2041 cccgcatgcc accgaaacgc gctgcctgcc gccgggcgac gtcctcgtag cccatgcccg
     2101 cggcgcccag cctggtgagc gcgtccgtga accgcgaaac atacggccac cattccgacg
     2161 gcagcccgtc gaggttgaaa tcgaggtgca ggtaggccac gccgttggac ggcacgtcgt
     2221 tgatcagcag gggaatgccg gggccgacac gttcgaggtt cgacgggatt tcggccgggg
     2281 ttccgggcag gtcgtgcagc ccgagttgcg gcaggagggc cagcgcctcg ggcggattgg
     2341 gcgtgccgga ttcgcggtcc agttcctcgg cctcggccgc tacgcgctgc accgccgcat
     2401 cgtcgagccc cgcccgaacc gtcttcatgc gctccgcgaa ttccgcgtcc agccgagcct
     2461 ggtgcccgcg gtccggccgc agcgccaact gcagccggtg cggattatcc agcagccgct
     2521 cgcggatcag gcggctgaag aacagcggat cggccgccgc ctccgcgcgc agggccgcga
     2581 gcgcgtcgcg catggacagg aagcgcagcg gctcgtcgga ataggtccac gcctgaagca
     2641 cacggaagag cacgtgcagc ggatagcccg attggacctc ctggtactcg tagccggcct
     2701 ggtgaaacgc ggcgtcgacg gcctccgggg cgaacggctc ttccgccagg ccccgaagcg
     2761 tatcgagaac gagcttctcg aaagcggcgg cccggtcggg ctcggagccc ttgagcccga
     2821 cgcggaaggt catctccagc ccggccggct ccgcgccgca cgagatgaca tcctcgccga
     2881 gcttcgaatc cacgagcgcc ttcttgaggg gcgcggcctc gtgcccgaag aggagcagcg
     2941 agagcaggta gagcgcggcc gtgtcgcgcg cggccgccgc gtcgccggcc agccaatcca
     3001 gccggaagta cgtgcggccg gaagcgggtt cgtcgtgtcc gatggcgtag gtttccgtgg
     3061 tcgcgcgggg tgcaggccag cgcggctggc gcgccggcac gggcgcggcg tcgcgcgcgg
     3121 agtacccggc gagccgctcg gcgagaaacg ccaggtagtc ctccgtcggg atgttgccgt
     3181 agcagacgaa caggccgcgg gcggggtggt agcaggtcga gtggaaattc cgaaactgct
     3241 cgaaggtcag gtcggggatg gcgcgcgggt cgccgccgga ctcgcgcccg tagatcgtat
     3301 ccggcagcag gctccggagc gcgatcatct ccatggccca ctcgggattc gagtagacgc
     3361 ccttcatctc gttgtagacg atgccgctga cggtgagccc gccggcgccg tcgggggcga
     3421 ggtgatggcc ctcgcgtttg aagatgttct cggtcagcag cgggtggaag accgcgtcga
     3481 agtacacgtc cgcgaggttg aagaggtcct gcttcacgtt gctggcgacg gggtagaccg
     3541 tgcggtccca gaaggtcatg gcgttgatga acgtggccat gctgatcttg accagctcga
     3601 aaaacggatc gcggaccggg tacttgtgcg aaccgccaag gacggaatgt tcgaggatgt
     3661 gcgggagccc cgtgtcgtcg gcgggcggcg tggggaaggc gacgcagaag aggttctccg
     3721 ggtcgtcgtt gacgaggtgg aggacgcggg cgcccgacgc accgtgccgc agttcgacgg
     3781 cccggcccag gggcgcagga aggggcgtgg ttgtgacgac ggtgaaaccg cgatgcgtgg
     3841 ttccgggttg gagcatggga ttatccttcg tacagggtgt cgtcgccggc cgggccgccg
     3901 ccctccatcc cttccatttc cgcgccgagc ttttccgggt cctccccggc ctcgagccgg
     3961 cgcatcatct gctccatttc cggcggcacg ggctcgccgg tctcctcggc catcttcctc
     4021 atggcgcggc cgagggagcg gggatcgttt tcatcgaggc cgtccagcgc ggaaaggtcc
     4081 gcgccctccc cgccggcgct gccggatttc acggacgcga agcgggacaa tacgcgcgtc
     4141 gtttccggat ggccgcagcg cgggcaggcc ggcggcttgt gggcggacgg attgcgtacg
     4201 aggaagctcg aaacccgtcc gcattttttg cattcgtact cgaataccgg catggcggcc
     4261 tccggaagcg gagtcagtat agagcagcga tgagcaaata caaccgcgcc atgccggccg
     4321 agcgtcgtcg gccccatgaa tcatagtcca tcaaccgtga cgcacgggtc gaggccgggg
     4381 aaaaaacaat cgcggcccgg atggaacgtc cgggccgcga ggcggaacag ccgatttcgg
     4441 agcgccggga tcagcgcttc gagaactgga agcgtttgcg ggcgccgggc tggccgtact
     4501 tcttgcgctc cttcatgcgc gggtcgcggg tcaggaaccc gccgcccttg agcttctcgc
     4561 gcagggccgc gtcggccagc agcagggcgc gggcgatccc gtgccgcagc gcgccggcct
     4621 ggcccatgat gccgccgccg gagacgttcg caatgacgtc gaaccgcttc agcatgtcgg
     4681 cggcctcgaa gggctgctcg atgaactggc ggaccgcctc cacggggaag taatccttga
     4741 agtccctgct gttgacggtg gtcttcccca cgccgtgcgc gatgcgcacg cgcgccacgg
     4801 aagtcttgcg ccgccccgtc gccgaatatt ccaccacttt gtgtgccacg ttcgttcccc
     4861 cgtctgtgtc agaccgccag ggccttcgcg ccctgcgccg cgtgcggatg ctccgcgccg
     4921 gcgtatacct tcagcctcgt gatcaacttc cggctcaaat ggttcttcgg gagcatgccc
     4981 ttgaccgcct gccggacgat gcgggtgggc tggcggacgc ggatgacgct ggccttctgc
     5041 atcttcaggc cgctcgggta gccggagtaa tccttgtaga ttttctgctc ttccttcgag
     5101 cccgtcagct tgacctgcgc cgcgttgatg accaccacga aatcgcccat gtcggcgtgg
     5161 ggggtatagt tgcgcttgtt ccggccgcgc aggatgttgg cgatcttgac cgccaggcgc
     5221 ccggccggct gaccggcggc gtccaccacg tgccacgcgc ggacgacctc cgattccctg
     5281 accaacgtag ttttcatgaa tctgcctttt ttttccgcaa acaggccgca aaacctagcg
     5341 gaaggggccg ccaggtgtca atccccatat ttgcgcgccg ttttcgtttg attccggcgg
     5401 cccgcaccgc tactcctctt tccatgcaac cccttcccct catcggcgcc gcggaactgc
     5461 cggcgtgcct cgatcgccgc cgccggccgc accagcagca ctacttcgcc atgtattcca
     5521 gccacctcgg cggcctggtc accgaccccg ccctcatgct gctgccggcc gacgaccaca
     5581 tggtccaccg gggcgacggc atcttcgaga ccgtcaagtg cgtcggcggg ggtctctaca
     5641 acctggaggc ccacctcacc cgcctgcgcc tctcggccgg gcgcatccgc ctgcccgtgc
     5701 cctggacgtc cgcggacctg gcggacatcg tggtccagac cgtccacgca ggcggccggc
     5761 cggaggccct cgtgcgcatc ctggtctcgc gcgggcccgg cggattcggc atcagccccg
     5821 ccgagtgccc ggagcccgcg ctctacgtcg tcgcctatgc cctccccggc tcgttcatga
     5881 cccttcaccc cggcggcgcc cgcgcggccc ggagctcgat cccgccccgc gccccgccct
     5941 tctcccatct caagagcgtc aactacctgc ccaacgtcct gatgaagatg gaggcccttg
     6001 acgcgcgcgt ggacttcacc ctgtccttcg acgagaagaa caacctggcg gagggcgcta
     6061 cggagaacgc ggggatcgtc acgcgcgggc gccgcctgca ggtgccgaaa cccgaccgca
     6121 tcctgcccgg cacgacggcc cgccgcgtcc tggaactggc ccgcccgttg atcgcgggcg
     6181 gcctgctttc cgccgtggag gagacggaca tcccctacgc cgccgtccgg gaggccaccg
     6241 agttgctcat cttcggcacg acgccggacg tcacctcggt catcgagttc gacggaaggc
     6301 cggtcggcga cgggaagccc ggcgcggcgg gcctcgcgct caacgcgcgg ctggcggacg
     6361 acatcctgca caacgcggcc ctgcgcacgc cggtcttctg accgcccggc ccgcggcggc
     6421 ccggctacag gttcggcccc gtgctgtgct tgaggacgtc gttgaactgg tcctcggtca
     6481 tgatcttctt ttccagcacc agctggcgca gcgtccggcc ggtctccagc gattccctga
     6541 ccagttcggc gacgcggtcg tagcccagtt tcgggttgag gacggtggcc aggccggcgc
     6601 tgtgctcgaa gtgccgcgcg cattcctcgc ggttcgcctg gatgccgcgc acgcatttct
     6661 cgccgagcag ggcgcaggcg cgctcgaggt agcgggtcgc ccgcaggagg ttgaatccga
     6721 tcagcggcat ataggtgttc agctccagct gacccgctcc gcaggccgcg gccacggccg
     6781 cgtccagccc gaagacctgg aggcaggcca tgttgacggc ctcgcaggcg ctgggattga
     6841 acttgccggg catgatcgag gagcccggct cgagcggcgg gaggatgatt tcatccaggc
     6901 ccgtgttcgg gccggaggag aggagccgca ggtcgttgca c
//
LOCUS       DAOOTK010000146         6941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6750_length_6941_cov_3.880046, whole genome shotgun sequence.
ACCESSION   DAOOTK010000146 DAOOTK010000000
VERSION     DAOOTK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6750_length_6941_cov_3.880046"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PK634_12605"
     CDS             complement(<1..774)
                     /locus_tag="PK634_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOW98617.1"
                     /translation="MDLQKLYAERIGGAQFGLGTEIYKFEKIKRAKAAALKARPQVEL
                     LDFGVGEPDQLAPFPIREALKKAVDDPANRGYADNGIREFRLAACRYMETFFGVKGLN
                     PDTEVNHSIGSKPALAMLPLCFVNPGDVVMTTVPGYPVMATHAKYLVAEIVKLPLERS
                     NGFFPDLKKIKPAVAKRAKLLYVNYPNNPTGTAATEAFFDELIAFAAKHNILVVQDAA
                     YATLIYGRPRLSILGRPGGREVAVELHSMSKSYNMTGWRL"
     gene            complement(853..3012)
                     /locus_tag="PK634_12610"
     CDS             complement(853..3012)
                     /locus_tag="PK634_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOW98618.1"
                     /translation="MAFRRRAAIFGFLLLSAAAGQAQLTAIWANDGGDKVARSECRAS
                     ANPGSVTNSLWDGSSVRLFGARNEVLGFALILEAASAAATNVSVVLTDLNRGDYRLRA
                     APAPLDGLFAWTNREIELFYLRYLQIQGLSQMAYETYDERHIPQRLRRPWTGEGEGEG
                     YWTNRPDHDQFYPDIAVPMELVPRFDVAAGESQVLWADVYIPKTTPAGAYTGAALVAQ
                     GGATQAVPVSLRVLDFALPDEPAARTMVYLGYRDLNVRYLDEPWPNEGTTNAALSGEI
                     RDRHFLLAHRHRISLIDANDGPEVWPDDAPRPGWLPRLTGELFTPAHGYAGPGEGAPN
                     GVFSIGTYGTWGWQDEGQTGMWAHTDAWAGWLATNAPGTEAFLYLVDESSDYPQIEQW
                     ASWMDANPGPGSNLFSMATLSLPDAVAHVPSLDIPAAIITMGISNDWETAATGLATNP
                     SRRLYLYNGVRPATGSFATEDDGIALRALAWAQHKKRVDRWFFWESTYYNNFQAGAGE
                     TRVLQSAATYGDSSETNDVLGETGWNHSNGDGVLFYPGTDRVYPEDSYGVPGPFASLR
                     LKLWRRGLQDADYLAMAARMNPTQTAQIVQRMVPKVCWEYGVADINDPTWCRTDISWS
                     VNPDDWEAARAELAGLIKSHLAAFRVRFAPSAGLTWADLGPGYAFTLQSAASLAAPAW
                     TSAPGAWPARTNEWAGLGAGTAAFFRVQASPLAPPGR"
     gene            complement(3135..3590)
                     /locus_tag="PK634_12615"
     CDS             complement(3135..3590)
                     /locus_tag="PK634_12615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732433.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOW98619.1"
                     /translation="MTPELMIGWGKFGGAAALAFGAMGSALGAGAAGPAAIGAWKKCF
                     AQGKAAPFILVTFIGAPLSQTIYGMILMNSIAEAVKNTPTSFPMMIAVGVFGGIAIGL
                     SAWYQGVIGAAAADAQAETGKGFGNYLMGLGIAETVALLVMVFSMMAIK"
     gene            complement(3612..5375)
                     /locus_tag="PK634_12620"
     CDS             complement(3612..5375)
                     /locus_tag="PK634_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98620.1"
                     /translation="MIVKMSKVTVLCVASAKDEALDALRDLGVLHVVPLKPLAGELLD
                     EARARLQHVRRALEMLDPKAQGKPSGKTADDVVEGVWKLIHRIKEDEDALVQLRHEQQ
                     RLAPYGHFEPADLDRLREKGITVRLYQAPRSQAVVIPEGAVRVVLSEDKTNVYFAVSG
                     PGDFQIDAQELKRPERSLKDVEEGLRWSEARLEESQRRLQEYSNDYAVVACTAAKAEE
                     TVDFLEARQGMEAGGPVAWLQGFCPEEHVPDVQAAAAKNGWGLLVGEPTADDKAPTLI
                     RNPFWVKPIKAVFDFIGVLPGYEEIDISAVFLLFLSLFFAMLVGDAGYGLIFLGLTFW
                     GRRKFPKAPGYPFHLLYIMSFGTVVWGLLTGSLFGMEALPAWLDRMTVAWLKEPKNVM
                     LLCFLIGAVHLTIAHLWNISRAWNSLKSLAQLGWIGSTWTMFFMARNLVLGHELPGWI
                     GWVFLVSVVLIVLFMTAPRDFKNEWFNHVMLPLNIVSNFVDVVSYIRLFAVGTAGYAV
                     ASSFNKMILGGGVHGIVAGLIAALLLFLGHTLNIILSAMGVLVHGVRLNTLEFSSHIG
                     MAWTGVPYRPFRRIESTHKES"
     gene            complement(5372..5965)
                     /locus_tag="PK634_12625"
     CDS             complement(5372..5965)
                     /locus_tag="PK634_12625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOW98621.1"
                     /translation="MPRIKHTKNELKAQRDALKRFERYLPTLQLKKQQLQMEVRRLEG
                     QIEQKRGEEEERRDGLKTWVRLFADAFPFGQYLKVAKVRASEGNIAGIAIPVLEEVRF
                     ERKMPDLFLTASWVDDGLEVLEQLLRLGIERRILDEQHRRLAEELRVTSQRVNLFEKV
                     KIPECGENIRVIRIFLGDEQTADVVRGKIAKGRTVTA"
     gene            complement(5968..>6941)
                     /locus_tag="PK634_12630"
     CDS             complement(5968..>6941)
                     /locus_tag="PK634_12630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOW98622.1"
                     /translation="RRMIRTGIPMIDVFNTLVESQKLPIFSVAGEPYNPLLARIALQA
                     EVDVIVLGGMGLKHDDYLFFRDYLEEHGALARAVMFVHTAADPTVECLMVPDISLAVA
                     EQFALQNKRVLVLLTDMTNFADSLKEIAITMEQIPSNRGYPGDLYSQLAARYEKAVDF
                     DTAGSITVLAVTTMPGDDVTHPVPDNTGYITEGQFYLRNGRIEPFGSLSRLKQLVNNK
                     TREDHRTIMNTMIQLYAGYKETLEKQAMGFRMSNWDRKLLKYGERFEKELMDLTVNIP
                     LEEALDLGWRILADCFEPAETGIPSKFIEKFWPKTAKTQEAVEAATK"
BASE COUNT         1133 a         2364 c         2246 g         1198 t
ORIGIN      
        1 cagccgccag ccggtcatgt tgtagctctt gctcatcgag tgcagctcga ccgcgacctc
       61 cctgccgccc ggccggccga ggatcgagag ccgcggccgc ccgtagatca gcgtcgcgta
      121 ggccgcgtcc tgtacgacca ggatgttgtg cttcgcggca aacgcgatga gctcgtcgaa
      181 gaacgcctcc gtcgccgccg tgccggtcgg gttgttcggg tagttgacgt agagcagctt
      241 ggcgcgcttc gcgaccgcgg gcttgatctt cttgaggtcc gggaagaagc cgttggaccg
      301 ctcgagcggg agtttcacga tttccgcgac gaggtacttc gcgtgcgtgg ccatcacggg
      361 atagccgggc acggtggtca tgacgacgtc gccgggattg acgaagcaga gcggcagcat
      421 ggccagcgcg ggcttcgagc cgatgctgtg gttgacctcg gtgtccgggt tcaggccctt
      481 gacgccgaaa aacgtctcca tgtaccggca ggcggcgagc cggaactcgc ggatgccgtt
      541 gtcggcgtag ccgcggttcg cgggatcgtc cacggccttc ttcagggcct cgcggatcgg
      601 gaacggcgcg agctggtccg gctcccccac cccgaagtcc agcagttcga cctgcgggcg
      661 cgccttcagc gcggcggcct tggcgcgctt gatcttctcg aatttataga tctcggtgcc
      721 cagcccgaat tgcgcaccgc cgatccgctc ggcgtacagt ttctgcaagt ccatcctcgt
      781 ctccttccgg cggcgaaatt ccgcgcgtac atagcatatc ggcgcgccac ctgcacggag
      841 aaatgcgggc gctcaccgcc cgggcggcgc caggggcgaa gcctggaccc ggaagaacgc
      901 cgccgtcccg gcccccaacc ccgcccactc gttggtccgc gcgggccagg cgccgggcgc
      961 cgacgtccac gccggcgccg ccagcgaggc ggcggactgg agcgtgaagg cataacccgg
     1021 cccgagatcg gcccaggtca gcccggcgga cggcgcgaac cggacccgga acgcggccag
     1081 gtggctctta atgagaccgg ccagttccgc gcgcgccgcc tcccagtcgt ccgggttcac
     1141 ggaccagctg atatccgtgc ggcaccaagt cgggtcgttg atatccgcga cgccgtattc
     1201 ccagcagacc ttcgggacca tccgctgcac gatctgcgcc gtctgcgtcg ggttcatccg
     1261 cgcggccatg gcgaggtagt ccgcgtcctg gagcccgcgc cgccagagct tcaggcgcag
     1321 gctcgcgaac gggccgggca cgccgtacga atcctccggg tagacgcggt ccgtgccggg
     1381 gtagaacagc acgccgtcgc cattgctgtg gttccagccg gtttcgccca gcacgtcgtt
     1441 ggtctccgag ctgtcgccgt aggtcgccgc agattggagc acgcgggtct cgcccgcgcc
     1501 ggcctggaag ttgttgtagt aggtggactc ccagaagaac cagcggtcca cgcgcttctt
     1561 gtgctgcgcc cacgccagcg cccgcagcgc gatcccgtcg tcctcggtgg cgaaggagcc
     1621 ggtcgccggc cggacgccgt tgtagagata cagccgccgc gacggattgg tcgccaggcc
     1681 tgtcgccgcg gtttcccagt cgttgctgat gcccatggtg atgatggccg ccggaatatc
     1741 cagcgagggc acatgcgcca cggcgtccgg caaggagagc gtggccatgg agaagagatt
     1801 cgagcccggc ccggggttgg cgtccatcca cgaggcccat tgctcgatct gcggatagtc
     1861 ggacgactcg tcgaccagat agaggaaggc ctcggtcccc ggggcgttgg tcgcgagcca
     1921 gccggcccac gcgtcggtgt gagcccacat gccggtctgc ccctcgtcct gccagcccca
     1981 cgtgccgtac gtgccgatgg aaaacacgcc attgggcgcg ccctcgcccg ggcccgcgta
     2041 cccgtgagcc ggcgtgaaca gttctccggt caggcgtgga agccagcccg gccgcggcgc
     2101 gtcatcgggc cacacctcgg ggccgtcgtt cgcgtcgatc agcgatatcc tgtgccggtg
     2161 cgcgagcagg aagtggcggt cgcgaatctc gccggacagg gcggcattgg tcgtgccctc
     2221 gttcggccag ggctcgtcca agtagcgcac gttgagatcg cggtagccaa ggtataccat
     2281 cgtgcgggcg gcgggctcgt ccggcagcgc gaaatccagg acgcgcagcg aaaccggcac
     2341 cgcctgggtt gcgccgccct gggccacgag cgccgcgccg gtgtacgcgc ccgccggggt
     2401 tgtcttcggg atgtacacgt ccgcccacag gacctggctc tcgccggcgg cgacgtcgaa
     2461 ccgcggcacg agttccatcg gcacggcgat atcggggtag aactggtcgt ggtcggggcg
     2521 gttcgtccag tacccctcgc cttccccctc gccggtccag ggccgccgca gccgctgcgg
     2581 gatgtgccgc tcgtcgtagg tctcgtaggc catctggctc aagccctgga tctggagata
     2641 acggaggtaa aacagctcga tctcgcgatt cgtccaggcg aacaggccgt cgaggggcgc
     2701 gggcgcggcg cgcaggcggt agtcgccgcg gttcaggtcg gtcaggacca cggacacgtt
     2761 cgtcgcggcg gcggacgcgg cttccaggat cagcgcgaag ccgagcactt cgttgcgcgc
     2821 gccgaaaaga cggacggacg agccgtccca caggctgttg gtgacggagc cgggattcgc
     2881 cgaggcccgg cattccgagc gcgcgacctt gtcgccgccg tcgttggccc agatcgccgt
     2941 cagttgggcc tgccccgccg cggccgacag gagcagaaac ccgaagattg ccgcgcgacg
     3001 ccgaaaggcc atggatcgcc tccggggaat agatacccgg cgtgcgcgga aaacggaata
     3061 ccggggagaa gggagcaggt ttcggtgttc gggaaatggg tcatcccgaa caccggaacc
     3121 cgaacaccgg aaccttactt gatggccatc atgctgaaga ccatgacgag cagggcgacg
     3181 gtttccgcga tgcccaggcc catcaggtag ttgccaaaac ccttgccggt ctccgcctgc
     3241 gcgtccgcgg ccgccgcgcc gatgacgccc tggtaccagg ccgagagccc gatcgcgatg
     3301 ccgccgaaga cgcccacggc gatcatcatg gggaagctgg tcggggtgtt cttcaccgcc
     3361 tccgcgatgc tgttcatcag gatcatgccg tagatcgtct gcgaaagcgg cgcgccgatg
     3421 aacgtcacca ggatgaacgg cgcggccttg ccctgcgcga aacacttttt ccacgcgccg
     3481 atcgctgccg gccccgccgc gcccgcgccg agcgctgagc ccatggcgcc gaacgccagc
     3541 gccgccgccc cgccgaattt accccagccg atcatcagtt caggtgtcat ttcccggtct
     3601 ccttattctt ttcacgattc cttgtgggtc gattcaatcc gccggaacgg ccggtacggg
     3661 acgcccgtcc aagccatgcc gatgtgcgac gaaaattcca gcgtgttcaa gcgcaccccg
     3721 tgcaccagca cgcccatcgc gctcaagatg atgttcagcg tgtggccgag aaacagcagc
     3781 agggcggcga tcagcccggc cacgatccca tgcaccccgc cgcccaggat catcttgttg
     3841 aatgacgagg ccaccgcgta gcccgccgtg ccgacggcaa agagacggat gtacgagacg
     3901 acatccacga aattgctgac gatgttgagc ggcagcatga cgtggttgaa ccactcgttc
     3961 ttgaaatcgc gcggggccgt catgaacagc acgattaaca ccacgctcac gaggaacacc
     4021 cacccgatcc agcccggcag ctcgtggccc agcaccaggt tgcgggccat gaagaacatc
     4081 gtccacgtgc tgccgatcca gccgagctgc gccagcgatt tcaggctgtt ccaggcccgg
     4141 ctgatgttcc acaggtgcgc gatggtcagg tggacggcgc cgatgaggaa gcacagcagc
     4201 atcacgttct tcggctcctt gagccacgcc acggtcatcc ggtccaacca cgccggcaag
     4261 gcctccatgc cgaacagcga accggtgagc aaaccccaga ccacggtgcc gaagctcatg
     4321 atgtaaagca ggtggaacgg gtaccccggc gccttcggga acttgcgccg cccccagaac
     4381 gtcaggccga ggaagatcag gccgtacccc gcatcgccga ccagcatggc gaaaaacagg
     4441 ctcaagaaga gcaggaatac cgcgctgata tcgatctcct cgtagcccgg cagcacgccg
     4501 atgaagtcga acaccgcctt gatgggcttg acccagaacg gattgcggat cagggtcggc
     4561 gccttgtcgt cggccgtcgg ttccccgacc agcagccccc atccgttctt cgcagccgcc
     4621 gcctggacgt cgggcacgtg ctcttcaggg cagaacccct gcagccaggc gacgggtccg
     4681 ccggcctcca tgccctggcg cgcctcgaga aagtcgacgg tttcctcggc cttcgccgcc
     4741 gtgcaggcca ccacggcgta gtcgttgcta tattcctgca ggcgccgctg cgattcctcc
     4801 agtcgcgcct ccgaccagcg aagcccctcc tcgacatcct tcagcgaacg ctcgggccgc
     4861 ttcagctcct gggcgtcgat ctggaaatcc ccggggccgg acacggcgaa atagacgttg
     4921 gtcttgtcct cgctcaagac aacgcgcacc gcgccctcgg ggatgaccac cgcctggctg
     4981 cggggcgcct ggtacagccg cacggtgatc cccttctcgc ggagccgatc caagtccgcg
     5041 ggttcgaaat gaccgtaggg cgccaggcgc tgctgctcgt gccggagctg gaccagcgca
     5101 tcttcatcct ccttgatccg gtggatgagc ttccagacgc cttcgaccac gtcgtcggcg
     5161 gtcttcccag acggctttcc ctgcgccttg gggtcgagca tctccagggc ccgccggaca
     5221 tgctgcagtc gggcgcgcgc ctcgtccagc agttcgcccg ccagcggctt gagcggcacg
     5281 acgtgcagca cgcccagatc gcgcagcgcg tccagcgcct cgtccttcgc ggacgcgacg
     5341 cacaacaccg tcaccttgga cattttgacg atcatgcggt caccgtccgt cccttggcaa
     5401 tcttgccccg caccacgtcg gcggtctgtt cgtcgccgag gaaaatccgg atgacgcgga
     5461 tgttctcgcc gcattccggg atcttcacct tctcaaacag gttcacgcgc tgcgaggtca
     5521 cgcgcagttc ctcggccagc cgccggtgct gttcgtcgag gatgcgccgc tcgatcccca
     5581 gccggagcag ttgctcgaga acctccaggc cgtcgtccac ccacgaggcc gtcaggaaga
     5641 ggtcgggcat ctttcgctcg aaccgcactt cctccagtac tgggatggcg atgccggcga
     5701 tgttcccctc gctggcgcgg accttcgcga ctttcaggta ctgcccgaac gggaacgcgt
     5761 ccgcgaaaag acggacccat gttttcaggc catcccgccg ctcttcttct tcgccccttt
     5821 tctgttcgat ctgcccctcg agccgccgca cctccatctg cagttgctgt ttcttcaact
     5881 gcagcgtggg caggtagcgc tcgaagcgct tcagcgcgtc gcgctgggcc ttcagctcgt
     5941 tcttggtatg tttgatcctc ggcacggtta cttcgtcgcg gcctcgaccg cctcctgcgt
     6001 cttcgccgtc ttcggccaga atttctcgat gaatttcgac gggatcccgg tttccgcagg
     6061 ctcgaagcag tcggccagga tgcgccagcc gaggtcgagc gcctcttcca gggggatatt
     6121 caccgtcagg tccatcagtt ccttctcaaa ccgctcgccg tacttgagga gcttgcggtc
     6181 ccagttgctc atccggaagc ccatggcctg cttctccagg gtctccttgt agcccgcgta
     6241 gagctggatc atggtgttca tgatcgtccg gtggtcctcg cgcgtcttgt tgttgacgag
     6301 ctgcttgagg cggctcaagg agccgaacgg ctcgatgcgc ccgttgcgga ggtagaactg
     6361 gccctcggtg atgtagcccg tgttgtccgg gaccgggtgc gtcacgtcgt cgccgggcat
     6421 cgtggtgacg gccagcacgg tgatcgagcc cgcggtgtcg aagtccacgg ccttctcgta
     6481 gcgcgcggcg agctggctgt acaggtcgcc cgggtagccg cggttcgagg ggatctgctc
     6541 catcgtgatg gcgatctcct tgagcgagtc ggcgaagttg gtcatgtccg tcagcagcac
     6601 cagcacgcgc ttgttctgca gggcgaactg ctcggccacg gcgaggctga tgtccggcac
     6661 catgaggcat tccaccgtcg ggtcggccgc cgtgtggacg aacatcacgg cccgcgcgag
     6721 cgcgccgtgc tcctccaggt agtcccggaa gaagaggtag tcgtcgtgct tcaggcccat
     6781 gccgccgaga acgatgacgt ccacctcggc ctgcagggcg atgcgcgcca gcagcgggtt
     6841 gtacggctcg ccggccacgg aaaaaatcgg cagcttctgc gattcaacca gcgtgttgaa
     6901 aacgtcgatc atcgggatcc cggtgcggat catgcgccgg g
//
LOCUS       DAOOTK010000147         6914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6789_length_6914_cov_4.434757, whole genome shotgun sequence.
ACCESSION   DAOOTK010000147 DAOOTK010000000
VERSION     DAOOTK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6789_length_6914_cov_4.434757"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1796
                     /locus_tag="PK634_12635"
     CDS             3..1796
                     /locus_tag="PK634_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98623.1"
                     /translation="MDGARWPDATNSSRNPAAGIPMTTSRVAVAVYLRADLDADGDSL
                     PDGWEQYHFGSLDPWRTDDPDGDGFTNLQEFQDHSDPRDAASVPVPPAIDHTPLGDPQ
                     GVDAPWPVSATITDNVLVAQAVLRWQRNGEGWQSAAMTSAPASTVYSADIPAPGTNRD
                     LFVYQIEAVDAAGWRSTNGPHRFAVWVAALAVAPAGLTNILVIENTVTGRVLQVSNTG
                     GGSLRWTGGVERVGFRDDMELGATGWVHGGTADLWHLSARRVFSGTNAWYAGSDVAGE
                     YANGMDARLACGPVPVRGHATLTFRYYADTEVKNASQAYDGGIVELSTNGGVAYRRIE
                     PVGGYPYRIVGGSGSPFTNGTPCLAGTGGGWQSVSFDLAAWAGLDVLVAFRFGSDSSK
                     VSEGWYVDDVGVSPDTAAGDWVALSPATGTVGAASSEPADVTFDATGLTGGTVRAAML
                     RVASDAPGAPAEVPVSLFVLGWATDLDGDGMFDVWEWLHGLDPLDPSDAAGDDDGDGL
                     SNGEEAVADTDPGSDASYLRLAGLEESSGRVLTFPASTQRLYSISYTTNLMAPGWTEW
                     LTRLAATGEVMSVTDTNAGSRRVYRLGVERP"
     gene            1811..2650
                     /gene="lpxI"
                     /locus_tag="PK634_12640"
     CDS             1811..2650
                     /gene="lpxI"
                     /locus_tag="PK634_12640"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978206.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOW98624.1"
                     /translation="MEGPIPESLGIIAGRGAYPRLLAEAARREGVRRIFAVAFRWETD
                     PAIGRAADEVAWIRLGQLGAMLDAFRRSGIRQAVMAGQIKPTHLFSLAMDRALRELLA
                     RLPERNAHTIFGAVGEELKRVGVELLPAHLFMESAMPEPGLLGRRPPTDGERADIELG
                     LKVAKLTSGLEVGQTVVIKNGTILSVEAFEGTDAAILRAGRLGGPGAVVVKVAKRGHD
                     MRFDIPVVGMHTLRSLRKIRAAVLAVEARRTILLEREKVIGEADRMGLCLTAVTMGGP
                     REA"
     gene            2647..3597
                     /locus_tag="PK634_12645"
     CDS             2647..3597
                     /locus_tag="PK634_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOW98625.1"
                     /translation="MKMRRVKVGVVGVGSLGKSHARIYSELPEAELVGVCDVDAARAE
                     AMAREYGTKAFADMDELAEHVEALSVVVPTDRHHEVGLRLLDRGIHLLVEKPIAASAA
                     EAEALVARAQERKAILQVGHVERFNPVLSFLDGLARPPRFIEAHRLAPYPPPRPGLPP
                     RGTEVSVVLDLMIHDIEVILHLVRAPVREIRAVGVPVLSPTEDIANVRLEFENGCVAN
                     ITASRISPDRMRKIRVFLEDTYISLDYQNQAGELFRKSSGGISREEIPIEKGEPLALE
                     LRSFLGCVLRRDRPVVSGEHAAEALKLAVAITRRIREGSP"
     gene            3594..4727
                     /gene="lpxB"
                     /locus_tag="PK634_12650"
     CDS             3594..4727
                     /gene="lpxB"
                     /locus_tag="PK634_12650"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOW98626.1"
                     /translation="MSRTVLLVAGEVSGDMIAAGLVRAVRARAPDVRFVGIGGDELKA
                     AGLELRRHVRDMAVMGLGEVLRRYGFFRRVFRETVELARALKPDLVVLVDYPGFNLRF
                     AREARRLGLKVLYYVCPQVWAWHRSRIGLMAGVVDRLLAIFPFEPAVFEGTGLRVDFV
                     GHPLADEAARARAEPPAPLPWNGEPRVALLPGSRRQEIHRILPLMWRAAGLLEKRHPG
                     MSFILAAPSEQVAGLVRQGLAGLAGGPSRAEVVVGQTRQVLRQARAALVVSGTATIET
                     ALMECPQLVVYRTSWLTYAVGKRVVRVPHLGMVNLVAGRRLCPELIQHEATPEAMASA
                     LEPLLGDTPERAAMREGYREVIRALGEGGAADRAAGILLEEIG"
     gene            complement(4791..>5415)
                     /locus_tag="PK634_12655"
     CDS             complement(4791..>5415)
                     /locus_tag="PK634_12655"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOW98627.1"
                     /translation="TNNALAGAPIQIQIGPDLIMAGLSAPPTGCSGSPLAVDYAAANI
                     GVGNPPDECYIGFYLSTDTVITTGDTLLGTRGIYGGFPPGTTNADAISVNLPAGLSPG
                     IYYLGAIVDVFNVNPEFSETNNSRASDPVYLAPAARVVNREASTVSLSFSGLRVSSVY
                     HVQRTAALLPEPTWTNTGAAITGVVSTNWSETVSEDASRAFYRLVEE"
     assembly_gap    5416..5515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5516..6020)
                     /locus_tag="PK634_12660"
     CDS             complement(<5516..6020)
                     /locus_tag="PK634_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOW98628.1"
                     /translation="MNRRMAAIVMLLGAAPAVRATALPDLIVTALTGPASGSTAASIT
                     ISNTVANVGAGNLVDECYIGFYLSTDTVVTTGDILLGNRGIYGGILPGGTNSGSANVG
                     VPAWLAAGTYYLGAIADYDSRNAESDEGNNAFAGSSIAIQIGPDLVMTQVSGPSTGHT
                     AAAISIDY"
     gene            complement(6166..6238)
                     /locus_tag="PK634_12665"
     tRNA            complement(6166..6238)
                     /locus_tag="PK634_12665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6203..6205),aa:Lys,seq:ctt)
     gene            complement(6293..6367)
                     /locus_tag="PK634_12670"
     tRNA            complement(6293..6367)
                     /locus_tag="PK634_12670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6333..6335),aa:Gly,seq:gcc)
     gene            complement(6448..>6914)
                     /locus_tag="PK634_12675"
     CDS             complement(6448..>6914)
                     /locus_tag="PK634_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOW98629.1"
                     /translation="FSCIQPMYNLVKRQAEVELFPMARAEKLGVLVYSPLGGGLLTGK
                     YRTGEHFKDSRLVHSDLYRRRYGPAWMHDAAREFTALARSRGFHPAALAVAWAAAHPA
                     VTAPILGARNLEQLEPALQAADIPMTPELYQAVSRLTPAPPPATDRSESAAG"
BASE COUNT         1015 a         2203 c         2535 g         1061 t
ORIGIN      
        1 cggtggatgg cgcccgctgg ccggacgcca cgaactcgtc gcggaatccg gcggccggaa
       61 tccccatgac cacttcccgg gtggccgtgg ccgtctacct gcgcgcggac ctcgatgcgg
      121 acggcgattc gttgccggac ggctgggagc agtaccattt cggctcgctc gatccctggc
      181 ggacggatga tcccgacggg gacggcttca ccaacctgca ggagttccag gaccatagcg
      241 atccccgcga cgcggccagt gtgccggtcc cgcccgcgat cgatcacacg cccctcggcg
      301 acccgcaggg cgttgacgcg ccctggcccg tcagcgcgac gatcacggac aacgtgctcg
      361 tggcgcaggc cgtgctccgg tggcagcgga acggcgaagg ctggcagagc gcggccatga
      421 cctccgcgcc ggcgtcgacc gtgtactcgg cggatatccc ggcgccggga acgaatcgcg
      481 acctgttcgt gtaccagatc gaagccgtgg atgccgccgg ctggcgctcc acgaacgggc
      541 cgcacagatt cgccgtttgg gtggcggcgc tggcggtggc gccggccggc ctgacgaaca
      601 tcctggtcat tgaaaacacg gtcaccggcc gggtgctcca ggtatcgaac acgggcgggg
      661 ggagcctgcg ctggacgggc ggcgtggagc gcgtcgggtt ccgggacgac atggaactcg
      721 gagcgacggg ctgggttcac gggggaacgg ccgacctctg gcatctctcg gcgcggcgcg
      781 tgttttccgg gacgaacgcc tggtacgccg ggtcagacgt cgccggcgag tacgccaacg
      841 gcatggatgc ccggctggcc tgcggccccg tccctgtccg cggccacgcg acgctgacct
      901 tccgctatta cgccgacacg gaagtcaaga acgccagcca agcctacgac gggggcatcg
      961 tcgagctttc cacgaacggc ggcgtggcgt accgccggat cgagccggtc ggcggctacc
     1021 cgtaccgcat cgtcggcgga tcgggctcgc cgttcacgaa cggaacaccc tgcctggcgg
     1081 ggacgggcgg cggctggcag tcggtttcct tcgatctcgc cgcgtgggcc gggctcgacg
     1141 tgctggtcgc cttccggttc gggtccgatt cgagcaaggt ctcggaaggc tggtacgtgg
     1201 acgatgttgg cgtgtcgccg gacacggcgg ccggcgattg ggtcgcgctg tccccggcga
     1261 ccgggacggt cggcgcggcg tcctcggagc ccgcggacgt gaccttcgac gcgacggggc
     1321 tcacgggcgg gaccgtgcgg gcggcgatgc tgcgggtcgc gtccgacgcc cccggcgcgc
     1381 cggccgaggt gccggtctcg ctcttcgtgc tggggtgggc gaccgacctg gacggggacg
     1441 gcatgttcga cgtctgggag tggctgcatg gcctggaccc gctggatccg tccgacgccg
     1501 cgggcgacga cgacggcgac ggcctctcca acggcgagga ggcggtggcc gataccgacc
     1561 cgggctcgga tgcctcgtac ctgcgcctcg cgggcttgga ggagagttcc ggacgcgtgc
     1621 tcacgttccc ggcctcgaca cagcggctgt attcgataag ttacacgacc aacctgatgg
     1681 cgcccggctg gacggaatgg ctgacgcgcc tggccgcgac cggcgaggtc atgtccgtta
     1741 cggacaccaa cgcgggttcg aggcgggtgt accggttggg cgtggagcgg ccgtagggcg
     1801 gaaaggacgg atggaggggc cgataccaga atcgttgggc atcatcgccg ggcggggagc
     1861 ctatccgcgc ctgctggctg aagccgcgcg gcgcgagggc gtgcgccgga tctttgccgt
     1921 ggctttccgc tgggaaaccg acccggccat cggccgggcg gccgacgagg tggcctggat
     1981 ccgcctcggt caactcggcg cgatgctgga cgccttccgc cgcagcggca tccgccaggc
     2041 ggtcatggcg ggccagatca agcccacgca cctgttcagc ctggcgatgg accgggccct
     2101 gcgggaactg ctggcccgct tgccggagcg gaacgcgcac acgatcttcg gggcggtcgg
     2161 cgaggagttg aagagggttg gcgtcgagtt gctgccggcc catctcttca tggaatccgc
     2221 catgccggag ccgggcctgc tcggccggcg tccgccgacg gacggcgagc gcgccgacat
     2281 cgaactgggg ctcaaggtgg cgaagctgac gagcggcctc gaagtcgggc agaccgtggt
     2341 gatcaagaat ggcacgatcc tctccgtcga ggcgttcgag ggcacggacg cggcgatcct
     2401 gcgcgcgggc cggctcggtg gcccgggcgc ggtcgtggtg aaggtggcga agcgcgggca
     2461 cgacatgcgc ttcgacatcc cggtcgtcgg catgcacacg ctgcgctcgc ttcgaaagat
     2521 ccgcgccgcc gtcctggccg ttgaggcgcg ccggacgatt ctgctggagc gcgaaaaggt
     2581 gatcggggag gcggaccgga tggggttgtg cctgacggcg gtgacgatgg gagggccacg
     2641 cgaggcatga aaatgcgccg ggtcaaggtg ggcgtcgtgg gcgtgggcag cctgggcaag
     2701 tcgcatgccc ggatctactc ggaattgccg gaggccgaac tggtcggcgt ctgcgacgtc
     2761 gacgccgcgc gcgccgaggc catggcaagg gaatacggca cgaaggcctt cgccgacatg
     2821 gacgaactgg ccgagcacgt ggaggcgttg agcgttgtgg tgccgacgga ccgccaccac
     2881 gaggtcgggc tccgcctcct ggaccggggt atccacttgc tggtcgagaa gccgatcgcc
     2941 gcgtccgcgg cggaagccga ggcgctggtc gcgcgcgccc aggagagaaa ggcgatcctc
     3001 caggtcggac acgtggagcg attcaacccg gtgctgtcgt tcctggacgg cctggcacgg
     3061 ccgccgcgtt tcatcgaggc ccaccggctg gcgccgtatc cgccgccgcg gccgggcctg
     3121 ccgccgcggg gcacggaggt cagcgtcgtg ctggacctga tgatccacga catcgaggtc
     3181 attctccacc tggtccgcgc gcccgtccgt gagatccgcg ccgtgggcgt gcccgtcttg
     3241 agcccgacgg aggacatcgc caacgttcgc ctggagttcg agaacgggtg cgtggcgaac
     3301 atcacggcca gccggatcag tcccgatcgc atgcggaaga tacgcgtgtt cctggaggac
     3361 acgtacattt cgctcgatta ccagaaccag gccggcgagc tcttccgtaa atcgtccggc
     3421 ggcatctccc gcgaggagat accgatcgaa aagggcgagc cgctggcgct cgagttgcgt
     3481 tcgtttctcg gttgcgtcct gcgccgggac cggccggtcg tctcgggcga acatgcggcc
     3541 gaggccctca agctggccgt ggcgatcacc cggcgcatcc gggaagggag tccgtgagcc
     3601 gcaccgtcct cctggtcgcc ggcgaggtat ccggcgacat gattgccgcg ggccttgttc
     3661 gcgcggtgcg cgcgcgggcg ccggatgtgc ggttcgtcgg gatcggcgga gacgagttga
     3721 aggccgccgg cctggaactg cgccgccacg tgcgcgacat ggcggtcatg ggcctgggcg
     3781 aggtgctccg gcgttacggg ttcttccggc gcgtgttccg cgaaacggtg gagctggcgc
     3841 gcgcgctgaa gcctgatctg gtggtgctcg tggattatcc cgggttcaac ctccggttcg
     3901 cgcgcgaggc ccggcggctg ggcctgaaag tgctctacta cgtgtgcccg caggtctggg
     3961 cgtggcatcg ctcgcgcatc gggctcatgg ccggggtcgt ggaccgcttg ctcgccatct
     4021 tcccgttcga gcccgccgtc ttcgagggca ccggcctacg ggtggatttc gtggggcatc
     4081 cgctggccga cgaggccgcg cgggcgcgcg cggaaccccc ggcgcccctg ccgtggaacg
     4141 gcgagccgcg cgtggccctg ctgccgggca gccgccggca ggaaatccat cgcatcctgc
     4201 ccctgatgtg gcgcgcggcc gggttgctgg agaagcgcca tccggggatg tcgttcatac
     4261 tggccgcgcc ctcggagcaa gtggccggcc tggtccggca ggggttggcg ggtctggccg
     4321 gcgggccctc ccgcgccgag gtggttgtcg gacagacccg ccaggtcctc cggcaggcgc
     4381 gcgccgcgct ggtggtctcc ggcacggcga ccatcgagac cgcgctgatg gaatgcccgc
     4441 agctcgtggt ctacaggacg tcctggctca cgtatgcggt gggcaaacgg gtggtgcgcg
     4501 tgccgcacct gggcatggtc aacctggtcg cgggccgccg gctctgtccg gagttgatcc
     4561 agcacgaggc cacgcccgag gccatggcgt cggcccttga gccgctgttg ggcgatacgc
     4621 ctgaacgcgc cgcgatgcgg gagggctacc gggaagtcat ccgcgccctc ggcgagggcg
     4681 gggccgcgga ccgcgcggcg ggcatccttc tcgaggaaat aggctgaacc gggatagccg
     4741 gcagagcgat cgtgccgctg gcgtgggaag cgccggccag ttggtgtgtt ttattcctcg
     4801 acaaggcggt agaaggcgcg cgacgcatcc tcggaaaccg tctccgacca gttggtcgaa
     4861 acgaccccgg tgatcgcggc cccggtattg gtccaggtcg gctcggggag cagggcggcc
     4921 gtccgctgga catgatacac ggacgacacc cgcagacccg agaaggacag ggaaacggtg
     4981 ctggcttccc ggttcacgac ccgcgcggcc ggcgcgaggt agaccgggtc cgaagcccgg
     5041 gagttgttgg tctcgctgaa ctcggggttc acgttgaaga catccacgat ggcgcccagg
     5101 tagtagatgc cgggtgacag cccggcaggg aggttgaccg agatggcgtc ggcattggtc
     5161 gtgccggggg ggaacccgcc gtagatgccg cgggtgccga gcagggtgtc gccggtggtg
     5221 atgaccgtgt ccgtggaaag atagaacccg atgtaacact cgtccggcgg gttgccgacg
     5281 ccgatgttgg ccgcggcgta atccacggcg aggggcgatc cgctgcaacc cgtcggcggc
     5341 gcgctcaacc cggccatgat caggtcgggc ccgatctgga tctggatggg tgcgccggcg
     5401 agggcgttgt tggtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgtaa
     5521 tcgatcgaga tggctgcggc cgtgtggccg gtcgacgggc cgctcacctg ggtcatgacc
     5581 aggtcgggcc cgatctggat ggcgatcgag ctcccggcga aggcattgtt gccctcgtcc
     5641 gactcggcat ttcggctgtc atagtccgcg atcgcgccca ggtagtacgt gccggcggcc
     5701 agccacgcgg ggacgccgac gttggcgctg cccgagttcg tgccgccggg caggatgccg
     5761 ccgtagatgc cgcgatttcc gagcaggatg tcgcccgtgg tgacaacggt atcggtcgag
     5821 agatagaaac cgatatagca ctcgtcgacc aggttgcccg ctccgacgtt ggccacggtg
     5881 ttgctgatgg tgatcgacgc ggccgtgctg ccggaggccg gccccgtcag cgccgtcacg
     5941 atgaggtcgg gaagcgccgt ggcgcggacc gcaggcgcgg ctcccaggag catcacgatg
     6001 gccgccatcc ttctgttcaa gacgattcct tccgtgcgcg ggcggacccg cggatacgaa
     6061 atagtagtcc gatgccggcc tcctgttaag aggctcggcg ccccctgccg cgagcgccgc
     6121 ggcatacgcg acagaacgcc ggattccgat ccggtaaggg gggaatagag gctgcggggc
     6181 tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct aagcctccgg
     6241 gatcgacaat accggaaagc ggcccggaat tcaacagggg aaataaaaaa aatggagcga
     6301 gcgaagggac ttgaaccctc gaccctcacc ttggcaaggt gacgctctac cactgagcta
     6361 cgctcgcatc caacgggcgc gcgaacaacg ctgtcccgat tacgcatcgc atacttacac
     6421 cacgaggccg cgccccgcaa gcgtttttta tcccgccgcg ctctcgctgc gatccgtggc
     6481 gggaggcggg gcgggggtga ggcggctgac ggcctggtac aattccggcg tcatgggaat
     6541 gtccgccgcc tgcagcgccg gctccagttg ttcgaggttc cgcgccccga ggatgggcgc
     6601 ggtcacggcc gggtgggcgg ccgcccaggc caccgccagg gccgcgggat ggaatccgcg
     6661 cgaccgggcc agggccgtga actcgcgggc cgcgtcgtgc atccacgccg ggccgtagcg
     6721 gcggcggtag aggtcgctgt gaacgagccg gctgtccttg aaatgctccc cggtccggta
     6781 cttgccggtc agcaggccgc cgcccagcgg gctgtacacg agcacgccga gcttctcggc
     6841 ccgggccatc gggaagagct cgacctccgc ctgccgcttc acgaggttgt acatcggctg
     6901 gatgcagctg aagc
//
LOCUS       DAOOTK010000148         6901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6803_length_6901_cov_5.220129, whole genome shotgun sequence.
ACCESSION   DAOOTK010000148 DAOOTK010000000
VERSION     DAOOTK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6803_length_6901_cov_5.220129"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK634_12680"
     CDS             <1..250
                     /locus_tag="PK634_12680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOW98630.1"
                     /translation="ILDYLKTDGREIHWDLIRLAMRSKANTVVFPLQDIIGLGTEARM
                     NVPGTTSGNWIWRFTWEQLTPAMEERLRQLTEETGRVA"
     gene            297..1556
                     /locus_tag="PK634_12685"
     CDS             297..1556
                     /locus_tag="PK634_12685"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOW98631.1"
                     /translation="MLNQPPREREWLAWLAVAAWTLAIYLAIPLARAIQSWVTEHWGR
                     AAFLHGVFAALAAGAGLALLGLARSRGPGRGRAAAWVAGVAILYAFLSWRLRANPEEA
                     VHFIEYGVLGFLLFRAFSHRVRDPLVYLAAGWLGAILGTTDEIIQWMTPRRVFGLRDV
                     WLNVQAVGLVQVLLAAGLRPACVRGPVAARSVRLVGGLGVAHLALLALCLSNTPPRVE
                     RYTLRWPVLEPLRRAYSMMAEYGYRHTDPEVGVFFSRFSREDLRSLDRARVEEVAARL
                     AEFRDPGRYEEFLRTWTPVTDPFTHEARVHLFRRDANAARAWAMRGDPEALRGHATVA
                     WRENRLLELYFGNALRLSGCVLPDRVARLLDEHHDPARPYTSPVSASLITRLTERQVR
                     EIFLVGMLILFLAERWAVRVIRNRGRA"
     gene            1553..2500
                     /locus_tag="PK634_12690"
     CDS             1553..2500
                     /locus_tag="PK634_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOW98632.1"
                     /translation="MTAWNISRRGFLGTMAAAGAAWSLGGPRRRAWAAPVPSRESFRF
                     VFFTDVHTRLEWETPRALDMAAAAMRAQAPDLVVAGGDLITDGLDSAAEQVAPRWDAF
                     MEMWNSLPGPRESAIGNHDLVAARPKDGSPASSDPRAIFRGKMGVPRTYRSFEAGGCH
                     FVLLDGIQVTDTERNCRGFIEPEQLDWVRADLEGVDPSTPIVLVTHMPLLTAFYQAVE
                     GATAPAPDNRVIVNNREVLELFGKHNLLLVLQGHLHVDEMIRWRRTTFITGGAVSGAW
                     WRGPRRGTDAGFGVVTLKGGRVEWEYVHYGWTPRRPPDA"
     gene            2636..3256
                     /locus_tag="PK634_12695"
     CDS             2636..3256
                     /locus_tag="PK634_12695"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOW98633.1"
                     /translation="MNRSDSRPSKQMGGDVADARQVRRWVQQARAGDEEAFGELMKMY
                     HQRIFGLACGMVNNPDDARDLSQQAWVKAWKNLSRYRESSSFYTWLYRIVSNTCLDFL
                     RHRARLGEQALPENVDLVTQSDPNPAVSRRSRPDQEAERAETKRLFDEALGELSPEHR
                     MTLTLREIEGLPYGKIAEVMGCRTGTVMSRVFYARRHMQRKLRGLL"
     gene            3570..3764
                     /locus_tag="PK634_12700"
     CDS             3570..3764
                     /locus_tag="PK634_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98634.1"
                     /translation="MGVGLRFRPDRETAAPVLAHAPASAVEWVETGLPKASTMVYEDQ
                     ESGMVVIWVVEQNGGENVGS"
     gene            3751..4209
                     /locus_tag="PK634_12705"
     CDS             3751..4209
                     /locus_tag="PK634_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98635.1"
                     /translation="MWARKQLGVGLAALSAAALWLSSGSPARAQDVVSLQATMIHASD
                     KPAAQDPRLDNIEYKLRRTLRFEYYKHIGEGSASVSLPGATVLSLGGGFQLNIAAADA
                     GKGKVRAAVQWMRGGDVVLNTTVVMNRGAPVVLGGISHEGGTLIVTLVAQ"
     gene            complement(4216..5952)
                     /locus_tag="PK634_12710"
     CDS             complement(4216..5952)
                     /locus_tag="PK634_12710"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOW98636.1"
                     /translation="MRPDRAELDLDNPHKGFILWGTDYAAGEPDNYYGATVYHVYMPW
                     REVETADQVFAWGSFETNHLKPILDDHPLATFILRPVADYPDGESSGMTGFYTGGELQ
                     RDFPRFLIEAPLNIPYWTYTSCAGDGPGWTPDWNHAAMIAQMTQYVAAIRARYDGDPR
                     ITAVQAGMLGLWGEWHQSGCEDHAPSNAAKIAVRDAYRLFTNTPVQIRYARDPDSMGV
                     PFGFHEDFFPSFTGPCLYGFPDCEDSGDWNLHYGFTHVNTAAVHNWKSNPVTGESPND
                     DQKQCWSNDFNDVMTVIRDYHFTGLGPAGGHEWNGNQARLQAMKRVLGYNLHLDRVAW
                     PDRVWKDWPFHVTLAFTNSGSAPCYHRFPVQLALCQADGTPAWTGALSCDLRQVRPGM
                     TLEHTQLVTVSGVATGTYSLRVGILDPRTGQPGVRLQTAGEDSFRRCACGPVLVTPDP
                     EHCDSNGDGVPDAWYLAYGLDPTNPTIGHADSDGDGLANREEWLSGTHPTNGQSFLRV
                     AGLHPLDDGPGIALRWLGASGIHYSVERTTNLLSTAETIATNVAGAGGWITNSDPEAA
                     GPGPRFYRVGVE"
BASE COUNT         1058 a         2150 c         2517 g         1176 t
ORIGIN      
        1 gatcctcgat tacctcaaga ccgacggccg cgagatccac tgggacctca tccggctggc
       61 catgcgctcg aaggccaaca cggtggtgtt cccgctgcag gacatcatcg gcctgggcac
      121 cgaggcccgc atgaacgtgc ccggcacgac cagcgggaac tggatctggc gcttcacctg
      181 ggagcagttg accccggcca tggaggagcg gctccgccag ttgaccgagg aaaccgggcg
      241 ggtggcttga acggatcgcg cccgcgctgc ggccggtccg gcggaggagc gttcggatgc
      301 tgaaccagcc gccgcgcgaa agggagtggc tggcctggct ggccgtcgcc gcctggacgc
      361 tggcgatcta cctggccatc ccgctggccc gcgcgatcca gtcctgggtg accgagcact
      421 ggggccgcgc cgccttcctg cacggggtgt tcgcggcgct ggccgcgggc gcggggctgg
      481 cgttgctcgg gttggcgcgc tcgcgcgggc ccgggcgcgg ccgggccgcg gcctgggtgg
      541 ccggtgtcgc gatcctgtac gcgtttctgt cctggcgact gcgcgccaat cccgaagagg
      601 cggtgcattt catcgagtac ggtgtcctcg ggttcctgct cttccgggcg ttcagccatc
      661 gggttcgcga cccgctggtc tacctggccg cgggttggtt gggggcgatc ctgggcacaa
      721 cggacgagat catccagtgg atgaccccgc gtcgcgtctt cgggttgcgc gacgtgtggc
      781 tgaacgtcca ggccgtcggc ctggtccagg tgctgctcgc cgcaggcctg cggccggcct
      841 gcgtgcgggg tcccgttgcg gcgcgttcgg tccggctcgt cggcgggctg ggcgtggcgc
      901 acctggccct gctggcgctg tgcctgtcca acacgccgcc gcgcgtggag cgctacacgc
      961 tgcgctggcc ggtccttgag ccgttgcgcc gcgcgtacag catgatggcc gagtacgggt
     1021 atcgccacac cgatcccgag gtgggtgttt ttttctcgcg gttcagccgg gaggatctcc
     1081 ggagcctgga ccgcgcccgg gtcgaggagg tggccgcgcg gctcgccgag ttccgcgacc
     1141 cgggccgcta cgaggaattc ctccggacct ggacgccggt gaccgatccg ttcacgcacg
     1201 aggcgcgcgt gcatctcttc cgccgggacg cgaacgcggc gcgggcctgg gcgatgcggg
     1261 gcgatcccga ggcgctgcgc ggccacgcga cggtggcctg gcgggagaac cgcctgctgg
     1321 agttgtattt cggcaacgcg ctccgcctgt ccggttgcgt gctgccggat cgcgtggccc
     1381 ggctgctcga cgagcatcac gatcccgcgc gcccgtacac gagcccggtc agcgcctcgc
     1441 tcatcacccg cttgacggag cggcaggtca gggaaatatt cctcgtgggc atgctcatcc
     1501 tgttcctggc ggaacgatgg gccgtgcgcg tgatccggaa cagggggcgt gcatgacagc
     1561 atggaacatc tcccggcggg gattcctggg gacgatggcg gcggctggag cggcctggag
     1621 cctgggcggg cccaggcgcc gggcctgggc ggcgccggtt ccgtcgcgcg agtcgttccg
     1681 gtttgtattt ttcacggacg tgcacacccg gctggaatgg gagacgcccc gggccctgga
     1741 catggcggcg gcggcgatgc gggcgcaggc ccccgatctc gtcgtcgcgg gcggcgacct
     1801 gatcaccgac gggttggact ccgcggccga gcaggtcgcc ccgcgctggg acgcgttcat
     1861 ggagatgtgg aattccctgc ccggccccag ggaatccgcc atcggcaatc acgacctggt
     1921 ggccgcccgt ccgaaagacg ggtctccggc gagctccgat ccgcgggcga tttttcgagg
     1981 gaaaatgggg gtgccgcgga cctatcgctc gttcgaagcc ggcggctgcc atttcgtgct
     2041 gctggacggc atccaggtta cggacaccga gcggaactgc cgcgggttca tcgagccgga
     2101 gcaattggac tgggttcggg cggacctcga aggagtggac ccgtcgacgc cgatcgtgct
     2161 cgtaacgcac atgccgctcc tgacggcttt ttaccaggcg gtggaagggg ccacggcgcc
     2221 ggcgccggat aaccgcgtca tcgtgaacaa ccgcgaggtg ctggagctgt ttgggaagca
     2281 taacctgctg ctggtgctgc aggggcatct gcatgtggac gagatgatcc gctggcggcg
     2341 gaccacgttc atcacggggg gcgcggtcag cggagcctgg tggcgcggcc cgcggcgcgg
     2401 gacggacgcc gggttcggcg tggtgacgtt gaaaggcggg cgcgtggaat gggagtatgt
     2461 ccactacggc tggacgccgc ggaggccgcc ggatgcctga cccggcgcag ttttttgagg
     2521 cctgcggttg tcccgcgttc aacaaacgcg ggacagggcc tttggcgttt tcaccggccg
     2581 ggttttcctc tagtatgacc gcgcgggtgg ggcaggagca cggtgtgccg gaacaatgaa
     2641 tagaagcgat tccaggccgt ccaaacagat gggcggagac gtggccgatg cccggcaggt
     2701 gcgccgatgg gtgcagcagg cgcgggccgg cgacgaggaa gccttcggcg aactgatgaa
     2761 gatgtaccac cagaggatct ttgggctggc atgcgggatg gtcaacaacc cggatgacgc
     2821 ccgcgacctg tcccagcagg cctgggtcaa ggcctggaag aatctctccc gctaccggga
     2881 atcctcctcc ttttacacgt ggctgtaccg gatcgtgtcg aacacctgcc tcgattttct
     2941 ccgccaccgc gcccggctcg gtgagcaggc gctgcccgag aacgtggacc tggtgacgca
     3001 gtccgatccg aatcccgccg tcagccggcg gtcacgccca gaccaggagg ccgagcgggc
     3061 ggaaacgaag cggctatttg acgaggccct gggcgaactt tcgccggaac accggatgac
     3121 gctgaccctg cgcgagatcg aggggctgcc gtacgggaag atcgcggagg tcatgggttg
     3181 ccgcacggga accgtgatgt cgagggtgtt ttatgcgcgc cggcacatgc agcggaaatt
     3241 aagggggctg ctatgaactg tcgcaaggcc cgccagtgga tcagcctgga tatggacggc
     3301 gaactctcgg tccgccggaa ggagcagttg tacaatcaca tccgccgctg cgggtcgtgc
     3361 aacgccgtcc gcgaccgttg gttgagcctg ggcgatcaca tgcgccagcg ggttggcgcg
     3421 ccgctcgaga cggccgaagc cgcctgggcg gatgtgcgaa gggccatcca tctcgcgaag
     3481 gacgaaggcg tggaggccgc tgcgcccggc tgggctggtc ttggcggtgg gcggctgcgg
     3541 cggcctccct gctggcgctg gcggcggtcg tgggggtggg gctccggttc cggccggacc
     3601 gggaaaccgc ggctccggtc ctcgctcacg cgccggcctc ggcggtggag tgggtggaaa
     3661 ccggcttgcc gaaggcctcc acgatggtgt acgaagatca ggagtcgggc atggtggtga
     3721 tctgggtcgt ggaacagaac gggggcgaga atgtgggctc gtaaacagct tggggtcggc
     3781 ctggcggcgc tgtcggccgc cgcgctctgg ctgtcctccg gatcgccggc ccgggcgcag
     3841 gacgtcgtct cccttcaggc gacgatgatc cacgcctcgg ataagccggc cgcccaggat
     3901 ccgcggctcg acaacatcga gtacaagctc cggcggaccc tccggttcga gtactacaag
     3961 cacatcggcg aggggtccgc ctcggtgagc ctgcccggcg cgacggtcct gtcactgggc
     4021 ggcggcttcc aactgaacat cgccgccgcg gacgccggca agggcaaggt gcgggccgcc
     4081 gtccagtgga tgcgcggtgg cgacgtggtg ctcaacacca cggtggtcat gaatcgcgga
     4141 gcgccggtcg tgctgggagg catctcccac gagggcggga cgctgatcgt gaccctggtc
     4201 gcgcagtagc gcttcctatt ccacgccgac ccggtagaac cgtggcccgg gcccggcggc
     4261 ctccgggtcg ctgttcgtga tccagccgcc cgcgccggcg acattcgtcg cgatggtctc
     4321 cgctgtcgag agcaggttgg tggtccgctc cacggaataa tggatccccg aggccccgag
     4381 ccagcgaagg gcgatgccgg gtccatcgtc cagcggatgc aggccggcca cgcggaggaa
     4441 ggattgcccg ttggtgggat gggtgcccga aagccattcc tccctgttcg ccaacccgtc
     4501 gccgtcgctg tccgcatggc cgatcgtcgg gttagtgggg tccagcccgt aggccaggta
     4561 ccaggcgtcc ggcacgccgt ccccgttgga gtcgcagtgt tccgggtccg gtgtcaccag
     4621 cacggggccg cacgcgcagc gacggaagga gtcttccccc gccgtctgga gtctcacgcc
     4681 gggttggccg gtgcgcggat ccaggattcc cacgcggagc gagtacgttc ccgtcgccac
     4741 gccgctgacc gtgacaagtt gggtatgttc cagcgtcatc ccgggccgga cctggcgcag
     4801 atcgcaggat agcgccccgg tccaggcggg cgtgccgtcg gcctggcaca gggccaactg
     4861 cacggggaat cggtgatagc agggggcgga gcccgagttc gtgaacgcga gtgtgacgtg
     4921 gaagggccag tccttccaca cgcggtcagg ccatgcgacg cggtcgaggt gcaggttgta
     4981 ccccagcacg cgtttcatgg cctgcagcct ggcctggttg ccgttccact cgtgcccgcc
     5041 ggccgggccg aggccggtga agtggtaatc gcggatcacc gtcatcacgt cgttgaaatc
     5101 gttcgaccag cattgcttct ggtcgtcgtt cgggctctcg ccggtgaccg ggttggattt
     5161 ccagttgtgc acggccgcgg tgttcacgtg cgtgaagccg tagtggaggt tccaatcgcc
     5221 ggaatcctcg cagtcgggaa acccgtagag gcagggcccg gtgaaggagg ggaagaagtc
     5281 ctcgtggaag ccgaacggca cgcccatgga atccggatcg cgggcgtagc gtatctgcac
     5341 gggggtgttg gtgaacagcc ggtaggcgtc gcgcacagcg atcttcgccg cgttgctcgg
     5401 ggcatgatcc tcgcaccccg actggtgcca ctcgccccag aggccgagca tcccggcctg
     5461 gaccgcggtg atgcgtggat cgccgtcgta gcgggcgcgg atggcggcga cgtactgggt
     5521 catctgggcg atcatggccg cgtgattcca atcgggcgtc cagcccggcc cgtcgcccgc
     5581 gcagcttgtg taggtccagt aggggatgtt cagcggcgcc tcgatcagga agcggggaaa
     5641 gtcgcgctgc agttcgccgc cggtgtagaa gcccgtcatg ccgctgcttt cgccgtccgg
     5701 gtagtccgct accgggcgca ggatgaaggt ggccagggga tggtcgtcga ggatcggctt
     5761 gaggtggttg gtttcgaatg aaccccaggc aaagacctgg tctgctgtct cgacctcgcg
     5821 ccagggcatg tagacatggt agacggtcgc cccgtaataa ttgtcgggtt cgccagccgc
     5881 gtagtccgtg ccccagagga tgaatccctt gtgcggattg tcgaggtcga gttcggcacg
     5941 gtccggccgc atatccgccg ggtgtgcgcg gccacagaac agcagaagaa tcagtccttg
     6001 ctggagaaac cggcctattc ccgatcgcga gacagccgtt ttactcacgc tgtggtcccg
     6061 gtattcgttg tgcctccctc ccgggcccgc gccatacttt gcaaagtgtg gcgcggggtg
     6121 atcaggattg acgccaagcc aatctcacga gagtggcccg caccgatgaa agagggcggc
     6181 gaatcctgta cgtgttgccg cgccgctcga cgtacccccg cctccgcagt ttgtccatgt
     6241 gccgtaaggc cgctcgcctg gaaattccgg tcttgtggga caacgaacga atcgtcccgt
     6301 ggcccctgga aagtgcctgg acaagttgaa cgcggcgcga gtgagtgaag ccggttaggg
     6361 cgaagcggat gtcctctatg gacgccttgc cgcttttata actatttatc atgcaggata
     6421 ataaagtggc ggcctgcggc atcgacgggt ccggggcagc gcgataaaaa acacggggcc
     6481 caacgcgcgt cggttggagc atgcctcggg cgttcaatat ccgcaggtat tgactcgcca
     6541 cggccacggg caggcgcacg ccctgggcga cctcggacac atgccgatag ggatggaaca
     6601 ccaagtagcg aagcaagagg agccgttgac gattggccag cgcgcggcag gtgcgccaca
     6661 gggtcggggc agccggtgct ggagcggatt ccaccatggc cacactttgc aaagtgtggc
     6721 catgggtgtc aagctatcct ggcggccggc aatggcgcga atctcggggt agaaaaagga
     6781 gcgttgacgg atcccggcac gcagttcgtg tggcgccgcg cggggaactg cgacccgccg
     6841 ccctgcgccg gcggccggtg tccgttcgtg tgggaggacc ggactttttg gtcgaacgag
     6901 a
//
LOCUS       DAOOTK010000149         6751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_6991_length_6751_cov_5.550777, whole genome shotgun sequence.
ACCESSION   DAOOTK010000149 DAOOTK010000000
VERSION     DAOOTK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_6751_cov_5.550777"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   22..379
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=22..53
                     /rpt_unit_seq="gtcgccccccgcgcgggggcgtggattgaaac"
     gene            466..1080
                     /locus_tag="PK634_12715"
     CDS             466..1080
                     /locus_tag="PK634_12715"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOW98637.1"
                     /translation="MKALIALLAVALLALPVAAEEPVDGVSGASWTEPSLPPLSDEEL
                     TQKITNWLAGHWMLTLATADRRGEPHASPVVYFSEGTTIYVRAKPDTNKIRNIEQNPS
                     VAYTVWEPAAKFEQVRSLQVSGRARVLRGAERDRVERIFRTAPGTQAAAIRAFLEKEG
                     ISPEQAFPEGEAWTIVAIEPIQARWIERSRSADEGQVWRTRPNR"
     gene            1197..3404
                     /locus_tag="PK634_12720"
     CDS             1197..3404
                     /locus_tag="PK634_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOW98638.1"
                     /translation="MKKQQTPLVVGNARFTVYAEGCVRLEYSHGARFAEGPSLLVGRK
                     PAKPAAAETALKGKTLTIRTPKLELVYTDDGQCFSAANLKIHHRNHTGHPMIWTPGKR
                     DGGNLGAVTRCLDQWKWCGGPAHFPVEGILSTDGGHFVQDEPRVYWSPKHDWPECLGH
                     AVQYDGYFFAYGNDYKGALKDFTTVFGKIPMVPRWTFGFWYSRWYAFTDKEFIGLVKR
                     YRQEGIPIDVMIIDTDWRHGWGGYDWNPKFFPNPRKALAALHKLGVQTSLNDHPGYDN
                     YDSLPADDSHLPAIAKRLGALPHQGQWSCDWSRKDAVQAWKDVLLGPRHDEGMDFWWV
                     DGWIKPPFGGTDSQLWANRMYYELAEERTGRRGMILARWGGVGSHRYPVQFSGDTPSE
                     WGVLQHQVEFTARSGNLGAVYWSHDIGGFFGREIDEELFIRWSQFGAMSPVFRTHSDH
                     GVREPWAFSPRARDIFRKQARIRYALAPYFYTLAREAHDTGLPLIRPLYLEYNDNDGG
                     ALWRRHQYLIGRDLLVIPADGPADRASGLYRKRAYFPPGVWTEIETGEAVKGMRDGHV
                     EIPIERIPTYARRGAIIPCQPPPAALGTAMPREIHFDYYPDPLAESAYELYEDDGATR
                     KCEKGEWARTTVKGRCRGAVIEMAVSAPRGKYAGMPAVREFVLRAALPEGARVKQAEI
                     RVGRGEWRKTRCRTAATCLAGTVQTPTRFAEARVRAGREGVAVRFTLDEVVND"
     gene            3496..3891
                     /locus_tag="PK634_12725"
     CDS             3496..3891
                     /locus_tag="PK634_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98639.1"
                     /translation="MKQPQAKQRKETRAPVLAEPEPFFSRTVIGNLLSIAAGLSFLFL
                     CMLLPLVGPAAMRGSGSPGAGAVSHARLNFLTFLAVLLFSMALSVMAYFSKMERRKKD
                     GSPQPIYTIGLFVVLLFLLVALFMGLLEI"
     gene            complement(3925..4848)
                     /locus_tag="PK634_12730"
     CDS             complement(3925..4848)
                     /locus_tag="PK634_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98640.1"
                     /translation="MNRATCLFLAGCLALSAGLFTARAAIPETPSGALMANYLRYLLL
                     DQVETQGADVIAQAPAADQAGLRAALDRWTGALQQTIRGDLEARLGDNARQQFEDFID
                     AFSRAEQAQDMAYLENLGRETGWPGPVSAYEDFRRWGVESWLASDMQGGVDLLSEMTA
                     RAQPAAAPPPRKSNPLRDAEAAPAAVEYEEPESGAPLQSFSSLREERRQKALEQAQAG
                     MQQVASEREAWEQEYASDVAAKAQAEAEAMKKQAERLAATDQEVLEQDKNSLKTKLIG
                     VLAGGVEAGVGGFTGAVGSRLADEAVNEIFK"
     gene            complement(4880..5782)
                     /locus_tag="PK634_12735"
     CDS             complement(4880..5782)
                     /locus_tag="PK634_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98641.1"
                     /translation="MKRWILLRWLCAATAALLCSCATPPQATPEEEEEAEIAEAVRQA
                     KLTSQLPRVMLLVDEQSLGTIPTAEVEAMAIDMLLEENVQVVDQDMVQSNLGKGQEVM
                     KMAGDNRGAAALGLQFGADLVVIGEVVAKPSARRISDTNLRSYQAAATLRAVRTDNSA
                     TIASASEDATVIGLDDVSGSAKAIKTAGKKSLDRLVPEMLDAWTRTGGRQQGKLNHVT
                     VTVGGVDQMWKLKAIREQLIDLKKQTANVVQRNYTAGVAVFELDSLVPIEELSETIVL
                     KPPEDIKFQVLEVGSGQINLKVAE"
     gene            complement(5795..6379)
                     /locus_tag="PK634_12740"
     CDS             complement(5795..6379)
                     /locus_tag="PK634_12740"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOW98642.1"
                     /translation="MKNFVRNMLFGVLAALFATGCASPKDKYFASTVSKANIYVMPKQ
                     YSVHKVAIMPFKAPTELIGNSVSDMMVTEMLRSERYELVERSQMAQVLNESELSLAGL
                     SAAKAVEIGTMLGADGVIIGTVDEYGTVAIGARAYPVVGISTRLIACDTGKVVWSVDL
                     AERAERRDVTLPEHARYMVHCMVAALYNEWRKSH"
     gene            complement(6391..>6751)
                     /locus_tag="PK634_12745"
     CDS             complement(6391..>6751)
                     /locus_tag="PK634_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98643.1"
                     /translation="RASTEKRLEPLGPKEENITSFEWRRTAIQKRTSEKDQWMRWDEM
                     VAHIEKWPKDQTSQRIVLSRRGSLPAMSYPARIDRRDVIINAPAKEESVEIQLGDVYD
                     TLFQRIEKAIRERTQAP"
BASE COUNT         1013 a         2231 c         2372 g         1135 t
ORIGIN      
        1 gctccaacgg cggcccggcc agtcgccccc cgcgcggggg cgtggattga aactgttgaa
       61 cgttctgtcg ctgccgacgt ccgggtgtcg ccccccgcgc gggggcgtgg attgaaacat
      121 ttctggcgcg ggatccctgt cgcgcgccgc tggtcgcccc ccgcgcgggg gcgtggattg
      181 aaactcacct ccgacgacga ccccataggt atggtaggtc gccccccgcg cgggggcgtg
      241 gattgaaacg tcgtgctgtc gccccagacg aacgatccgg ccggtcgccc cccgcgcggg
      301 ggcgtggatt gaaactcgcc gccttgtgga tagccataga actacacgtc gccccccgcg
      361 cgggggcgtg ggctttgcag tttttcggcc agctcgccgg cgcgagttga aaccccgcgg
      421 gcgcggggta tctttcatcg ttcctccacc gaaccggaga caacgatgaa agcattgatt
      481 gccctgctgg ccgttgcctt gctggccctt cccgtcgccg ccgaagaacc cgtggacgga
      541 gtgtcggggg cgtcctggac cgagccgtcg ctgcctccgc tgtccgacga ggagttgacg
      601 cagaagatca cgaactggct cgcggggcac tggatgctga cgctggccac ggccgaccgg
      661 cgcggcgagc cgcacgcgag cccggtcgtc tacttcagcg aggggacgac gatctacgtg
      721 cgggcgaagc ccgacacgaa caagatacgg aacatcgagc agaatccctc ggtggcctat
      781 accgtctggg agcccgcggc gaagttcgag caggtgcgct cgctacaggt cagcggccgg
      841 gcgcgcgtgc ttcgcggggc cgagcgggat cgcgtcgagc ggattttccg gaccgcgccc
      901 ggcacgcagg ccgccgccat ccgggccttc ctggaaaagg aagggatatc cccagagcag
      961 gcgttcccgg aaggcgaggc gtggacgatc gtcgccatcg agccgatcca ggcgcgatgg
     1021 atcgagcggt cgcggtcggc ggacgagggc caggtctggc ggacgaggcc gaatcggtag
     1081 aacgtcgtgg aggtttcgcc tccaaaaaat agcgcttgca ttcacgagcc agccgcgcat
     1141 aatcgccaat atttcctaat agtaaaacgc tttaactgcg aaaggcggac atggcgatga
     1201 agaagcaaca aaccccgttg gtcgtaggga atgctcggtt cacggtctat gcggagggct
     1261 gcgtccgctt ggagtacagc catggcgcgc ggttcgccga gggaccgtcg ctgctggtcg
     1321 ggcgcaagcc ggcgaagccc gcggccgccg agacggcgct gaagggcaag acgctgacga
     1381 tccggacgcc gaagctcgag ctggtctaca ccgacgacgg ccagtgcttc tccgcggcga
     1441 acctgaagat ccatcaccgc aaccacaccg ggcatccgat gatctggacg ccgggcaagc
     1501 gggacggcgg caacctgggc gcggtgaccc gctgcctgga ccagtggaag tggtgcggcg
     1561 ggccggcgca tttcccggtc gagggcatct tgagcacgga cggcgggcat ttcgtgcagg
     1621 acgagcctcg cgtctactgg agcccgaagc acgactggcc ggaatgcctc ggccacgcgg
     1681 tgcagtacga cggctacttc ttcgcctacg gcaacgacta caagggcgcg ctcaaggatt
     1741 tcacgaccgt gttcgggaag atcccgatgg tgccgcgctg gacgttcggc ttctggtact
     1801 cgcgctggta cgcgttcacg gacaaggagt tcatcggcct cgtgaagcgc taccgccagg
     1861 aaggcatccc gatcgacgtc atgatcatcg acaccgactg gcggcacggg tggggcgggt
     1921 acgactggaa cccgaagttc ttcccgaacc cgcggaaggc cttggccgcg ctgcacaagc
     1981 tgggtgtgca gacctcgctg aacgaccacc cgggctacga caactacgac agcctgcccg
     2041 ccgacgacag ccacctgccc gccatcgcga aacggctcgg cgcgctgccg caccagggcc
     2101 agtggtcctg cgactggtcg cggaaggacg ccgtgcaggc gtggaaggac gtcctgctgg
     2161 gcccgcggca cgacgagggc atggatttct ggtgggtgga cggctggatc aagccgccgt
     2221 tcggcgggac ggacagccag ctctgggcca accgcatgta ctacgagctg gccgaggagc
     2281 gcacgggcag gcggggcatg atcctcgcgc gctggggcgg ggtgggctcg caccggtacc
     2341 cggtccagtt ctccggcgac acgccgtcgg agtggggcgt cctgcagcac caggtcgagt
     2401 tcaccgcgcg cagcggcaac ctgggggcgg tctactggtc gcacgacatc ggcgggttct
     2461 tcgggcgcga gatcgacgag gagctgttca tccgctggtc gcagttcggc gcgatgagcc
     2521 ccgtgttccg cacgcacagc gaccacggcg tccgcgagcc gtgggcgttc agcccgcgcg
     2581 cccgcgacat cttccgcaag caggcgcgca tccggtacgc cctggcgccg tatttctaca
     2641 ccctcgcccg cgaggcccac gacacggggc tgccgctgat ccgcccgctg tacctcgagt
     2701 acaacgacaa cgacggcggc gcgctctggc ggaggcacca gtacctgatc ggccgcgacc
     2761 tgctggtgat cccggcggac ggccccgcgg accgggccag cggcctgtac cgcaagcggg
     2821 cctacttccc gccgggcgtc tggacggaga tcgagacggg cgaggccgtg aagggcatgc
     2881 gggacgggca cgtggagata ccgatcgagc gcatcccgac gtacgcgcgc cggggcgcga
     2941 tcatcccgtg ccagccgccg ccggccgcgc tgggcacggc catgccgcgc gagatccact
     3001 tcgactacta tcccgatccg ctcgcggagt ccgcgtacga gctgtacgag gacgacggcg
     3061 cgacccggaa atgcgaaaaa ggcgaatggg cccggaccac ggtcaagggt cggtgccggg
     3121 gcgccgtgat cgaaatggcc gtctcggcgc cgcgcgggaa atacgcgggg atgccggcgg
     3181 tccgcgagtt cgtgctgcgc gcggccctgc cggagggcgc gcgcgtgaag caggcggaaa
     3241 tccgcgtcgg ccggggcgag tggaggaaga cgcgctgccg gacggccgcg acgtgcctgg
     3301 ccgggacggt gcagaccccg acccggtttg ccgaggcgcg agtgcgggcg ggccgggagg
     3361 gcgtcgccgt ccggttcacg ctggacgagg tcgtgaacga ctgaccagat caccggtctg
     3421 tagccgggac cgccggtccc ggctgcagct tcccgcttga ttcaacggcg tggcgccggt
     3481 actgtccaac gcagcatgaa acaaccgcag gccaagcagc gcaaggagac ccgggcgccg
     3541 gttctcgcgg agccggagcc gtttttctcc cggacggtca tcggcaacct gttgtccatc
     3601 gccgcggggc tgtcgtttct gttcctgtgc atgctcctcc cgctggtcgg gccggcggcg
     3661 atgcggggct cgggctcgcc gggggcgggg gcggtttccc atgcccggct gaacttcctg
     3721 acgttcctgg ccgtgctgct gttttcgatg gcgctgtcgg taatggccta cttctcgaag
     3781 atggagcggc gcaagaaaga cggcagcccg cagccgatct acaccatcgg gctgttcgtg
     3841 gtgctgttgt tcctgctggt ggccctgttc atgggccttc tggaaatctg aagaaacggg
     3901 gcgagggggc cccgcctcca gggcttactt gaagatctcg ttgaccgcct cgtcggccag
     3961 gcgtgaaccg actgctcccg tgaacccgcc gacgccggcc tcgacgccgc cggcgaggac
     4021 gccgatgagc ttggtcttca ggctgttctt gtcctgttcc aggacttcct ggtccgtggc
     4081 ggcgagccgt tcggcctgtt tcttcatcgc ctcggcctcg gcctgcgcct tggcggcgac
     4141 gtcgctggcg tactcctgct cccaggcctc gcgctcgctc gccacctgtt gcatgccggc
     4201 ctgcgcctgc tcgagggcct tctggcggcg ctcttcccgc agcgaggaaa agctctgcaa
     4261 cggcgccccg ctctccggct cctcgtattc cacggcggcc ggcgcggcct ccgcgtcgcg
     4321 caagggattg gatttccggg gcggcggcgc cgcggccggc tgcgcgcggg cggtcatctc
     4381 gctcaacagg tccacgccgc cctgcatgtc ggatgccagc cagctctcca cgccccagcg
     4441 gcggaagtcc tcgtaggcgg acacggggcc cggccagccg gtctcgcgtc ccaggttctc
     4501 caggtaggcc atgtcctgcg cctgctcggc gcggctgaag gcgtcgatga aatcctcgaa
     4561 ctgctgccgg gcgttgtcgc ccaggcgcgc ctcgaggtcg ccgcggattg tctgctgcag
     4621 ggcgccggtc cagcggtcga gggccgcccg caggcccgcc tggtcggccg ccggggcctg
     4681 ggcgatgacg tccgcgccct gggtctccac ctggtcgagc aggaggtagc gcaggtagtt
     4741 ggccatcagc gcgccggacg gggtttccgg tatggcggcg cgggccgtga acaggcccgc
     4801 cgacagggcc aggcatccgg ccaggaacag acaggttgct cggttcatgc gccctcctgc
     4861 ccgcggcggg gcgcgggcgt tattccgcga ctttcaggtt gatctgaccc gacccgactt
     4921 ccagcacctg gaacttgatg tcttccggcg gcttcaggac gatggtttcc gacagctcct
     4981 cgatgggcac gagggagtcc agctcgaaga cggccacgcc ggcggtgtag ttgcgctgga
     5041 ccacgttggc cgtctgcttc ttgaggtcga tcaactgctc gcggatggcc ttgagcttcc
     5101 acatctggtc cacgccgccc acggtcaccg tcacgtggtt aagcttcccc tgctggcgcc
     5161 cgccggtccg ggtccaggcg tccagcatct cggggacgag ccggtcgagg ctctttttcc
     5221 cggccgtctt gatcgccttg gcgctgccgc tgacatcgtc gaggccgatg accgtcgcgt
     5281 cctcggaggc ggaggcgatc gtcgcggaat tgtcggtgcg gacggcgcgg agggtggccg
     5341 cggcctggta ggagcggagg ttggtgtcgc tgatgcggcg ggcggagggc ttggcgacca
     5401 cctcgccgat cacaacgaga tccgcgccga actgcaggcc cagcgcggcg gcgccccggt
     5461 tgtcgccggc catcttcatc acttcctggc ccttgccgag attggattgg accatgtcct
     5521 ggtccacgac ctggacgttc tcctccagca gcatgtcgat ggccatggcc tcgacctcgg
     5581 cggtggggat cgtgccgagg ctctgctcgt ccaccagcag catgacgcgc ggcagctggg
     5641 aggtcagctt ggcctggcgt acggcctcgg cgatctcggc ctcctcctct tcctccgggg
     5701 tggcctgggg gggtgtcgcg cacgagcaca gcagggcggc cgtcgccgcg cacaaccacc
     5761 gcagaaggat ccaacgcttc atggggcctc ccgattaatg gcttttccgc cactcgttgt
     5821 acagcgccgc gaccatgcaa tggaccatgt accgggcgtg ttcggggagc gtcacgtccc
     5881 tccgttcggc ccgctcggcc aggtccacgc tccagaccac cttgcccgtg tcgcaggcga
     5941 tcagccgcgt ggagatgccg acgacggggt aggcccgcgc gccgatggcg acggtcccgt
     6001 actcgtccac ggtgccgatg atcacgccgt cggcgccgag catggtgccg atctcgacgg
     6061 ccttggccgc ggagaggccc gcgagggaca gctcggattc gttgagcacc tgggccatct
     6121 ggctgcgctc gaccagctcg tagcgctccg agcggagcat ctcggtgacc atcatgtccg
     6181 acacggagtt gccgatcagc tcggtcgggg ccttgaacgg catgatggcg accttgtgca
     6241 ccgagtactg cttcggcatc acgtagatgt tcgccttgct gacggtggag gcgaagtact
     6301 tgtccttggg cgaggcgcag cccgtcgcga aaagggccgc caggacgccg aacagcatgt
     6361 tgcgtacgaa gttcttcatg ggtgttttcc tcaaggggct tgcgtgcgtt cccggatggc
     6421 cttctcgatg cgttggaaca gggtgtcgta gacgtcgcca agttggatct ccacggattc
     6481 ctccttggcc ggggcgttga tgatcacatc ccgccggtct atgcgggcgg ggtaggacat
     6541 cgccggcaga gagccgcgcc gggagaggac gatgcgctgg ctggtctggt ccttgggcca
     6601 tttctcgata tgggcgacca tttcatccca gcgcatccac tggtccttct cggacgtgcg
     6661 tttctggatg gcggtgcgac gccactcgaa gctggtgatg ttctcctcct tgggcccgag
     6721 cggctcgagt cgcttctcgg tggaggcccg g
//
LOCUS       DAOOTK010000150         6698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7052_length_6698_cov_4.473882, whole genome shotgun sequence.
ACCESSION   DAOOTK010000150 DAOOTK010000000
VERSION     DAOOTK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7052_length_6698_cov_4.473882"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK634_12750"
     CDS             <1..264
                     /locus_tag="PK634_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOW98644.1"
                     /translation="LFIGGSWVRALLLITLGIAVVGGLDNFLRPALVSGKTRMNGLVV
                     FISLLGGVAAFGFVGIVLGPVVAAAVISLLQLGAADAEGDGGA"
     gene            349..1248
                     /locus_tag="PK634_12755"
     CDS             349..1248
                     /locus_tag="PK634_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOW98645.1"
                     /translation="MQDKKLLKMARRLSDPFRVENGRKFRLKDADPGDTLWLKKADKE
                     RAKEGLRAGVEALAELQDRLYAQDQWAVLLIFQAMDAAGKDGAIKHVMSGVNPQGCQV
                     FSFKAPSAEELDHDFLWRCLKNLPERGRIGIFNRSYYEETLVVRVHPEFLARQQLPPR
                     LVTKRIWEERFEDIRNVERHLFRSGVLIRKFFLHASKKEQKKRFLERLDDPEKNWKFS
                     AADVAERAHWDEYMEAYEDMVRHTATPEAPWFVVPADNKWFTRMVVAAAVVEALASLK
                     LHYPVVSDAKRKELDAARRRLEG"
     gene            1252..1923
                     /locus_tag="PK634_12760"
     CDS             1252..1923
                     /locus_tag="PK634_12760"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOW98646.1"
                     /translation="MPEGRKGFFARHLDAGEIFGEILFGLIMALTFTLGAGISVTEGP
                     GAIRTLLVTALGCNLAWGIIDGAMYVMGALLERGRRIRMLLSLQQATDPAAALAAVAR
                     ELDPVLERVTTAEERAKLYRGILTMASRVEPERARITREDLAGAVASGLLVILTAFPA
                     AIPFMVMEDHHLALRASNAFLVAMLFFIGFFWARFTGVNRWRAGLLMMLSGLALVKIA
                     MLLGG"
     gene            1936..3429
                     /locus_tag="PK634_12765"
     CDS             1936..3429
                     /locus_tag="PK634_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98647.1"
                     /translation="MKCSIRWVLLLAAGLAGPLGANAAEEEPAPEAPGFASKLLWYVP
                     NRVMDFLDIFRLRLRVGPGLAANFRITDYGAFYAGKYTALYAGLPGPRYPHPVRWPVG
                     FESLTGIIIAGVDATDETRHGPEYGAPELDVGGHVLLLGVDAGIDPLEIGDFVGGFLL
                     RDWVGDDYPSRLPPSPEWSDGLSLGAGEGVFQVEPKPDRFASWSQRLDYLETNVQRRI
                     SEPLRATDAYFAMDPTNAIVPPQTQVRMKLFTRLIQGSRFSLNIEPDIELDVELPNIE
                     KRLRVFFESARADELPERELSENEEHGLNVGARRWFEDLYLSADAGVKAKWLPQAFAR
                     LTWARDFEPGKWDLRPMQRFFVNTDDGLGSLTSLRVNRWLGEANRAILINRTSGKWTS
                     ESGDFSWSESIGLAGAFRLLDEHRRGRSIGWEDTARALGIEYTVFGDEEAVNLHRVAL
                     GLRRPLYKYWVYGEAGPGIEWNRADDFDATFFLHLGIDMLFWGAAHR"
     gene            3506..4282
                     /locus_tag="PK634_12770"
     CDS             3506..4282
                     /locus_tag="PK634_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOW98648.1"
                     /translation="MKHWRNGFFGLAVFGLAGCSTVSLHEPLRVGVTPDLPPIIAKAD
                     GRIVGLEADFARRLARELRRPVEWVELKWEDQVPALLTGRIDLIMSGMSVTEMRKVRI
                     AFCEPYMTSGLMALTRREGAGKYRTREALLRADAKVGVKIGTTAELFAQEHFRNAQLL
                     RYEIPHDAALALRNRTLDIYLDDAPAIFWLASKYESDLTFSRARLTEEDIAWGVSPKA
                     AALKEQVNAILAGWREDGTLREILERWVPVAPKSFTRGAK"
     gene            complement(4296..5573)
                     /locus_tag="PK634_12775"
     CDS             complement(4296..5573)
                     /locus_tag="PK634_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98649.1"
                     /translation="MKSADHCPRMAEAPAYCLGELSPDKKADYVRHMAACPVCARAAA
                     SFQRVIARLREDPDAQCSPSLSDRIARRIEAGDTRPVRRGITWAIPARVAALLLATLG
                     LGVFLTRDNRREDPSDLSAARREALVWLCQAQNPAGGWSAGEGAIREKYGIGVSALAL
                     MALMQNPAESQREPTAAAIRRGVDFLVNAQNDKGLFGAVFSGATYNQGLATLALIEAC
                     ALESNVAWRASAEKAVAYLVSIQDRTGGWDYLHSAPGSANTSASVWPLLALLRAEELG
                     YPELRPAIERGVAWMKSTVNEEGHMGYRRANQSPYGTGTLTAAGVTCLARHGHPEDAR
                     LIETMAEAVRKTTPTSTPAMDYYRAFFTSEALASAPGATAGFLRAALREQLVSLQSRT
                     GPNRGSWEPTDRWGRTGGPVYSTALATLCMARD"
     gene            complement(5560..6096)
                     /locus_tag="PK634_12780"
     CDS             complement(5560..6096)
                     /locus_tag="PK634_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOW98650.1"
                     /translation="MHEDSEDFELMRRLRDGDPEAMTGLVRRYQGSLLNFFRRMGACT
                     DAEDAVQETFIRVYNYRDRYRPSAKFTTFLYTVARHVWHDLLRKVMRRESLAERITAE
                     PEPEQAPGPSGAALRLDIHAALARLPEKLRGVVVLGVYQGMRQEEIAAVLDIPVGTVK
                     SRMFNALERLKEAFDEER"
     gene            6234..>6698
                     /locus_tag="PK634_12785"
     CDS             6234..>6698
                     /locus_tag="PK634_12785"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOW98651.1"
                     /translation="MRTTGRWIMAGFLAWSLVPGVAPAAEEAVLPEVVVTASRTPRDP
                     QAEAATVYGLNTQEQTTRQGLRTTPDLLKGLPSAMIQKTSTGQGSPFLRGFTGFRTLC
                     LIEGIRLNNSVFRDGPNQYWNTVDPLSIARSEVVMGPGSVMYGSDAVGGTMNA"
BASE COUNT         1091 a         2180 c         2339 g         1088 t
ORIGIN      
        1 ctgttcatcg gcggcagctg ggtccgggct ctcctgctca tcacgctggg gatcgcggtg
       61 gtcggcgggt tggacaattt cctgcggccg gccctcgtca gcggcaagac ccggatgaac
      121 ggcctcgtgg tcttcatcag cctgctcggc ggcgtggccg ccttcgggtt cgtcggcatc
      181 gtgctcgggc cggtcgtggc cgccgcggtg atcagtctgc tgcaactcgg ggcggcggat
      241 gcagaagggg atggcggggc gtaggaactg cgaaacacgc gaaaggcgcg aaaaggagag
      301 ctctttcgcg tgtttcacgc ctttcgcagc taccgcagaa aggatgccat gcaggacaag
      361 aagctgctga agatggcgcg ccggctgtcc gatccgttcc gggtggagaa cggacggaag
      421 ttccggctca aggacgcgga ccccggcgac acgctgtggc tgaagaaggc ggacaaggag
      481 cgcgcgaagg aggggctgcg cgcgggcgtg gaggccctgg cggagttgca ggaccggctc
      541 tacgcgcagg accagtgggc cgtgctgctg atcttccagg cgatggacgc cgcgggcaag
      601 gacggcgcga tcaagcacgt catgtccggc gtgaatccgc agggctgcca ggtgttttcc
      661 ttcaaggcgc cctccgcgga agagctggac cacgatttcc tctggcgttg cctgaagaac
      721 ctgccggagc gcggccggat cgggatcttc aaccgctctt actacgagga gaccctcgtc
      781 gtgcgcgtgc acccggaatt cctggccagg cagcagttgc cgccgcgcct cgtgacgaag
      841 cgcatctggg aagagcgctt tgaggatatc cgcaacgtcg agcgccacct gttccgcagc
      901 ggcgtgctga tccgcaagtt ctttctacac gcctcgaaaa aggagcagaa gaagcgcttc
      961 ctcgaacggc tcgacgaccc ggagaagaac tggaagtttt ccgccgccga cgtggcggag
     1021 cgggcgcact gggacgagta catggaggcc tacgaggaca tggtccgcca cacggcgacg
     1081 cccgaggcgc cctggttcgt cgtgccggcg gacaacaagt ggttcacgcg gatggtcgtc
     1141 gcggcggcgg tcgtcgaggc gctggcctcg ctgaagctgc attatcccgt cgtcagcgac
     1201 gcgaagcgca aggagctcga cgccgcccgg cggcggctgg agggatgacg cgtgccggaa
     1261 ggcaggaaag gcttcttcgc ccggcacctc gatgcgggcg aaatcttcgg ggagatcctc
     1321 ttcggcctga tcatggcgct taccttcacg ctgggggcgg ggatctccgt gacggagggg
     1381 ccgggcgcga tccgcacgct gctggtcacg gcgctcggct gcaacctggc ctggggcatc
     1441 atcgacgggg ccatgtacgt catgggcgcc ctcctggagc ggggccgacg gatccggatg
     1501 ctgttgagcc tccagcaggc aacggacccg gcggcggcgc tggcggcggt ggcccgggag
     1561 ctcgatcccg tgctggagcg ggtgaccacg gcggaggagc gcgcgaaact gtaccgcggc
     1621 atcctgacga tggcctcgcg cgtcgagccc gagcgggcgc ggatcacgcg ggaggacctc
     1681 gcgggcgccg tcgccagcgg actgctggtg atcctgacgg ctttccccgc ggccattccc
     1741 ttcatggtca tggaggacca ccacctggcc ctgcgcgcat ccaatgcctt tctcgtggcc
     1801 atgctgtttt tcatcgggtt cttctgggcg cggttcaccg gcgtcaaccg ctggcgcgcg
     1861 ggattgctga tgatgctgtc gggcctggcg ctggtgaaga tcgccatgct gttgggcggg
     1921 taggatggga ccgttatgaa atgttcaatt cgatgggtgc tgctcctggc cgccgggctc
     1981 gccgggccgc tgggcgcgaa cgcggccgaa gaagagcccg cgccggaggc gccggggttc
     2041 gcatcgaagc tcctgtggta tgtcccgaac cgggtcatgg atttcctgga catcttccgc
     2101 ctgcgcctgc gcgtggggcc cggcctggcg gcgaacttcc gcatcacgga ctacggcgcg
     2161 ttctacgcgg gcaagtacac cgcgctctac gcgggactgc ccggtccccg ctacccgcat
     2221 cccgtccgct ggcccgtggg gttcgaatcg ctgaccggca tcatcatcgc gggcgtggac
     2281 gcgacggacg agacgcgcca cgggcccgag tacggcgcgc cggaactcga cgtcggcggg
     2341 catgtcctgc tgctcggcgt ggacgcgggg atcgatcccc tggagatcgg ggatttcgtc
     2401 gggggcttcc tgctgcgcga ctgggtcggc gacgactatc ccagccgcct gccgccgtcg
     2461 cccgagtgga gcgacggcct ttcgctgggg gcgggggagg gcgtgttcca ggtcgagccc
     2521 aagccggacc ggttcgcctc gtggagccag cgcctggact acctcgaaac caacgtgcag
     2581 cgccgcattt ccgaaccgct gcgggcgacg gacgcctact tcgccatgga cccgaccaac
     2641 gcgatcgtgc cgccgcagac ccaggtccga atgaagctgt tcacgcggct gatccagggc
     2701 agccggttca gcctgaacat cgagccggac atcgagttgg acgtcgaact cccgaacatc
     2761 gagaagcgac tgcgcgtgtt cttcgagtcg gcgcgggccg acgaactgcc cgagcgcgag
     2821 ctgtcggaaa acgaggagca cgggctcaac gtcggcgcgc gcaggtggtt cgaggacctg
     2881 tacctctccg cggacgccgg cgtgaaggcc aagtggctgc cccaggcctt tgcgcggctg
     2941 acctgggccc gggatttcga gccggggaaa tgggacctcc ggccgatgca gcgctttttc
     3001 gtgaatacgg acgacgggtt gggctccctg acctcgctgc gggtcaaccg ctggctgggc
     3061 gaagccaacc gcgccatcct gatcaaccgg acctcgggca aatggacgtc ggaatccggc
     3121 gacttcagct ggtccgagag catcggcctg gccggcgcct tccggctgct cgacgagcac
     3181 cggcgggggc gcagcatcgg atgggaagac accgcccgcg cgctggggat cgagtacacg
     3241 gtgttcggcg acgaagaggc cgtcaacctg caccgcgttg ccctgggcct gcgccgcccc
     3301 ctgtacaagt actgggttta cggggaggcg gggcccggga tcgagtggaa tcgggccgac
     3361 gacttcgacg ccaccttctt cctgcacctg ggcattgaca tgctcttctg gggcgccgca
     3421 caccggtgac cgggcggcac ggccgcattg cgctcgactt ccaaccgctc atcccggaaa
     3481 atcgagagcc ttgaaggagc gccctatgaa acactggcgg aatggatttt tcggactcgc
     3541 ggttttcggc ctggcaggct gcagcaccgt gtccctgcac gagcccctcc gggtcggcgt
     3601 cacgccggac ctgccgccga tcatcgccaa ggccgacggc cggatcgtgg gcctggaggc
     3661 ggatttcgcc cgccgcctcg cgcgggagtt gcgccggcct gtcgagtggg tcgaactgaa
     3721 atgggaggac caggtgccgg ccctgctcac cgggcgcatc gacctgatca tgtccgggat
     3781 gtccgtcacg gagatgcgga aagtccgcat cgcgttctgc gaaccgtaca tgacctccgg
     3841 cctgatggcg ctgacccgcc gggagggcgc ggggaaatac cggacgaggg aggccctgtt
     3901 gcgggcggac gccaaggtcg gcgtgaaaat aggcacgacg gccgagctct tcgcccagga
     3961 gcatttccgc aacgcgcagt tgctccgcta cgagatcccc catgacgccg ccctggcgct
     4021 gcggaaccgg acgctggata tctacctgga cgacgccccg gcgatcttct ggctggcgtc
     4081 caagtacgag tcggacctga ccttctcgcg ggcccgcctg accgaggagg acatcgcgtg
     4141 gggcgtgagc ccgaaggctg cggcgctgaa ggagcaggtc aacgcgatcc tggccggctg
     4201 gcgcgaggac ggcacgctcc gggaaatcct cgagcgctgg gtccccgtgg cgccgaagtc
     4261 ctttacccgg ggcgcgaagt aggcggcggg ctcgttcaat cgcgcgccat gcagagggtc
     4321 gcgagcgccg tggagtagac cggtccgccc gtgcggcccc agcgatccgt cggttcccaa
     4381 ctcccgcggt tcgggccggt tcgggattgc agggagacca gctgttcgcg cagggcggcg
     4441 cgcagaaatc cggcggtcgc gcccggcgcc gaggccaggg cttccgatgt gaaaaaggcc
     4501 cggtaataat ccatcgccgg cgtggacgtc ggcgtggtct tccggaccgc ctcggccatg
     4561 gtttcgatga gccgggcgtc ttccggatgg ccgtggcgag cgaggcaggt cacgccggcg
     4621 gccgtcagcg tgcccgtgcc gtacggcgac tggttggcgc gacggtaccc catgtggcct
     4681 tcctcgttga ccgtggattt catccaggcc acaccgcgct cgatggcggg tctcaattcc
     4741 gggtagccca gttcctcggc ccggaggagg gccagcaaag gccagacgct ggcggacgtg
     4801 ttggccgagc cgggcgccga gtgcagatag tcccagccgc cggtccgatc ctggatcgag
     4861 accaggtagg ccacggcctt ttccgccgac gcgcgccacg ccacgttcga ttccagcgcg
     4921 catgcctcga tcagggccag cgtggccagc ccctggttgt acgtggcgcc ggagaagacg
     4981 gcgccgaaca gccccttatc gttctgcgcg ttcaccagga agtccacgcc ccggcgtatg
     5041 gccgcggccg tgggttcccg ctgcgattcg gccggattct gcatcagcgc catcagggcc
     5101 agggcggaca cgccgatgcc gtacttctcc cggatcgccc cttcgccggc cgaccagccg
     5161 ccagccgggt tctgggcctg gcacagccag accagggcct cgcggcgggc ggccgacagg
     5221 tcggagggat cctcccgacg gttgtcgcgc gtcaggaaaa ctccgagacc cagcgtggcc
     5281 agcaacaggg ccgccacccg tgcgggaatc gcccatgtga ttccgcggcg aaccggccgg
     5341 gtgtccccgg cctcgatcct ccgcgcgatc cggtcggaca gggaggggga gcactgtgcg
     5401 tccggatcct cgcgaaggcg agcgatcacg cgctggaacg aggcggcggc ccgcgcgcag
     5461 acagggcacg ccgccatgtg ccgcacgtag tccgctttct tgtccggcga cagttcgccg
     5521 agacaataag ccggggcctc cgccatacgg ggacagtggt cagcgctctt catcgaacgc
     5581 ctccttcagc cgctccagcg cgttgaacat gcgggacttc accgttccga cagggatatc
     5641 gagcacggcg gcgatctctt cctgacgcat cccctggtac acgcccagga ccacgacgcc
     5701 gcgcagcttt tccggcaggc gcgccagcgc ggcatgaata tccagcctca aggcggcgcc
     5761 ggaaggcccg ggcgcctgtt cgggctccgg ctccgccgtg atccgctccg ccaaggattc
     5821 ccggcgcatc accttgcgca gcagatcgtg ccagacgtga cgcgcgacgg tgtagagaaa
     5881 cgtcgtgaat ttggccgagg ggcgatatct gtcccggtag ttatacacgc gaatgaacgt
     5941 ctcctgaacc gcgtcttccg catcagtaca agcgcccatc cgccggaaaa agttcagcag
     6001 ggagccttga taccggcgca cgagaccggt catagcctct ggatcgccgt cccgcaggcg
     6061 gcgcatcagt tcaaagtcct cggaatcctc gtgcatggca tggagttgct acgcgtgaag
     6121 attcacatag gttgaccgtc ctaagcaagt tcggcaccgt caaatcagaa tattttcccg
     6181 tgaactattt cccgggcccg gtcgcttaat cagaaaaagg aggaactgtg tccatgcgaa
     6241 caacgggccg atggattatg gccggatttc tggcgtggag ccttgtgcca ggtgtggcgc
     6301 ccgccgcgga ggaagccgtg ctacccgagg tggtcgtgac ggcgtcgcgt acgccgcgcg
     6361 atccgcaggc ggaggccgcg acggtgtacg ggttgaacac gcaggaacag accacccggc
     6421 agggactgcg gacgacgccg gacctgctga agggcctccc gtcggccatg atccagaaga
     6481 cctccaccgg ccagggctcg ccgttcctgc gtggctttac cgggttccgg acgctctgcc
     6541 ttatcgaggg catccggctg aacaattccg tgttccgcga cggacccaac cagtactgga
     6601 acacggtcga ccccctgtcc atcgcccgca gcgaagtggt catggggccc ggatcggtga
     6661 tgtacggcag cgacgcggtc ggcggcacaa tgaatgcc
//
LOCUS       DAOOTK010000151         6688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7066_length_6688_cov_5.145334, whole genome shotgun sequence.
ACCESSION   DAOOTK010000151 DAOOTK010000000
VERSION     DAOOTK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7066_length_6688_cov_5.145334"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK634_12790"
     CDS             complement(<1..190)
                     /locus_tag="PK634_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HOW98652.1"
                     /translation="MKYEQVSLGRVFVLRLEDGDVVHEVIERFAEEHNVRAASVIVVG
                     GADKGSRLVVGPEKGRAKN"
     gene            complement(187..1206)
                     /locus_tag="PK634_12795"
     CDS             complement(187..1206)
                     /locus_tag="PK634_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98653.1"
                     /translation="MGTWSDLSAWWKRLWNRRPSEEYLPVSPESDVGEAAAAPAKPGR
                     LIHWPVSRRDRQLAALQEGYVEMLGLMRGIREHLEKQQGVQEKMVQVLDRLPDSMDGL
                     KNVGRAAEQQVEVMSLLRKQIESGATHDQQLIDSMNRFNQTLGVMDQTSRSSGRAVEQ
                     LIVKAADSEKMLRDAMERSERRFMIVTAVFAVVAVLAAGAVMFLVWANRAASPREALP
                     APSIAREAPPPAASADASEALPPGAIPVPTEPELAPPAEPVPPAEPEPPPIAMEPPAS
                     EVSSVESPAPEVAPEPAPVVEPAPVESAGPAPAAVETDKPVKKSRRHSRRAPPEPAPA
                     EPEAP"
     gene            1344..2522
                     /locus_tag="PK634_12800"
     CDS             1344..2522
                     /locus_tag="PK634_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98654.1"
                     /translation="MNPSPITYFMVHSPLHEASAMQSLAGQCIHRLETVSLRPAPEGA
                     PASPGDEVLLLLPISGGTEKQMKDVRDAWDRAGRRPVWVLAIPHHSSLAASLEFLASL
                     HNTGIRGRVFYFDPAAGDDDPGFRAIAGAVEALRANRILHASRLGLIGMPSDWLLTSP
                     PDAELVRTAWGPELAYRPVEECLRALDAGQTMDQALKGLVSRHRLAALTLRCFDLLSA
                     RDMTACLSLARLNDEGIPAGCEGDIPSALAMMWVRALWNEPAWMANPSRLDLRLNRLT
                     LAHCTVPFRLTSGHTIRTHFESGRGEAIAGQWTSDEVTLFRIGGPRLGEWWIGQGRVI
                     ERGRDENLCRTQVTVEFDRADALPALLERPLGNHLVVIPGNRAAALDTALAIRKQLNP
                     "
     gene            complement(2542..3006)
                     /locus_tag="PK634_12805"
     CDS             complement(2542..3006)
                     /locus_tag="PK634_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOW98655.1"
                     /translation="MLELAVVSAAFMMGAAIPHKYSGGGENVSPPLEWGPAPQGTKSL
                     AVLCDDPDAPAGDWAHWVLFNLPPDTRRLEEGIPAAPQLPSGARQGLNDYNRSGYAGP
                     WPPPGRPHRYIFKVFALDTALNLDARARKADLLRAMEGHILAQGQLMGTFRR"
     gene            complement(3025..4326)
                     /gene="hisD"
                     /locus_tag="PK634_12810"
     CDS             complement(3025..4326)
                     /gene="hisD"
                     /locus_tag="PK634_12810"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOW98656.1"
                     /translation="MDIKVTRWSGGNSVPDVDRFLRRPAIDPAAEKKARRVLEEIRQQ
                     GDEAVVRFVREFDGAVLEPAGLAVSVEERKATIAQVDVDFKRAATEAHRRIVRFAQAG
                     LRKDWTIATPKGGVLGEQYVPLARVGCYVPAGAAPLASTALMTVTLARVADVREVVAC
                     SPAGKEGALDPYLLYSLDLAGATEIYKVGGIQAIGAMAYGTATIRKVQKIVGPGGPYV
                     TAAKRLVYGEVDLDMIAGPSEIAILADDTAPAEYVAADLLSQAEHGTGHEKALLVTPS
                     AALAESVRKELVRQTARLSRREAVERVLEEGTLLVVVDHLDAGMELCNRFAPEHLEIM
                     TREPRRWVSKVRAAGAVFTGAWTPESAGDFVAGPSHVLPTGGTAALFSGLSVDSFRRR
                     SSVISLTRADLQDVLPVIEAFGRVERLDAHAQSARIRFEQK"
     gene            complement(4350..4586)
                     /locus_tag="PK634_12815"
     CDS             complement(4350..4586)
                     /locus_tag="PK634_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98657.1"
                     /translation="MIPVSIFTLLWLPMAMALLVVLGLGAWYTRDGRVRARRKRASNI
                     YRCAVCRRVYVETRDLPLARCPHCSCMNEVVKRG"
     gene            complement(4583..5377)
                     /locus_tag="PK634_12820"
     CDS             complement(4583..5377)
                     /locus_tag="PK634_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98658.1"
                     /translation="MITGVAELPALPSRRAPAPLPPRPDRRHHTVSWGLGILLAVAWH
                     LAWLAGFSWHPETPRPAMASAPTLVYAATPEEAEDSADPRLLWSPALFSLPSAAGFSA
                     PALAERFSARPAAGVWESAPLMLERDGRTGRAGRGDSLQEEVAANLFLVPLPPLEATV
                     FRGPPRAPSASVEFRPGWSEDDFEDMPLPAGWGKTGDRAWEATAYLEMDAGGAAEHVF
                     LESPSECPEVNRLLEKALWNWRIKPGASAASGRVVFRAPGAGEEAP"
     gene            complement(5374..5841)
                     /locus_tag="PK634_12825"
     CDS             complement(5374..5841)
                     /locus_tag="PK634_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOW98659.1"
                     /translation="MSPSFQPEIERLSTLRRRFRTRLRPATGPMATVPLLNVFLLLWL
                     FHIVNSAFVLQPGIRMQLPAAPFTAGAHYGTRVVTLTQEGQIFFEDERVSLAELPAAL
                     AKAVGRTPSPLLIEADGRVRHQVLVHIYAMATEAGFREVLLATRLPERTAEAP"
     gene            complement(5849..6472)
                     /locus_tag="PK634_12830"
     CDS             complement(5849..6472)
                     /locus_tag="PK634_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOW98660.1"
                     /translation="MLDIMQKGGPVMWMILAASFLGVAVFFAKFYELHRAQIRSGDFL
                     KGIFNILNRHNIVEAVSICEETPGPVASVVRAAILHHDEERHAIAQAIEETGLAEVPR
                     LERRLTLLATLAQVAPMLGLLGTVMGMIHVLQVIEQKAPLIHAGDVLGGLWQALITAA
                     AGLAVGIPAYAGYNFLVSRVEAILLDMERAAVDILAYLTGPARRPGA"
     gene            complement(6506..>6688)
                     /locus_tag="PK634_12835"
     CDS             complement(6506..>6688)
                     /locus_tag="PK634_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98661.1"
                     /translation="ATQLGRNPGRLRNAAAILESQSKWREAVKVYKRVQEAGVPAAAE
                     AELRIRQIQSEHWILF"
BASE COUNT          993 a         2388 c         2326 g          981 t
ORIGIN      
        1 ggttcttcgc ccggcctttt tccggcccga cgaccagccg gctgcccttg tccgcgccac
       61 cgaccacgat gaccgacgcc gcgcggacgt tgtgctcctc ggcgaaccgc tcgataacct
      121 cgtgcaccac gtcgccgtcc tccaaccgga gaacgaaaac gcggcccagg ctgacctgct
      181 catatttcat ggcgcctccg gctccgcggg cgcgggctcg ggcggggccc gacgcgaatg
      241 ccgccgcgac ttcttcacgg gtttatccgt ttccacggcg gccggcgcgg ggccggctga
      301 ttccaccggc gcgggctcca ccacgggcgc cggctccggc gcgacctccg gggctgggga
      361 ttcaacggac gaaacttcgg atgccggcgg ttccatcgca atcggcggcg gctccggctc
      421 cgccgggggg accggttccg ccgggggcgc gagttcgggt tcggtcggaa cgggaatggc
      481 gccgggaggc aatgcctcgg aggcatcggc cgaagcggcc ggaggtgggg cttcccgcgc
      541 gatcgaaggg gcgggaagag cctcgcgggg cgaggccgcc cggttggccc acaccaggaa
      601 catcaccgcg cccgcggcca gcaccgccac gacggcgaag acggccgtga cgatcatgaa
      661 gcggcgctcc gaccgctcca tggcgtcgcg cagcatcttc tcggaatccg cggccttgac
      721 gatcaactgt tccaccgccc ggcccgacga ccggctggtc tggtccatga ccccgagcgt
      781 ctggttgaag cggttcatgc tgtcgatcag ctgctggtcg tgggtggcgc cggattcgat
      841 ctgcttgcgc agcagcgaca tgacctcgac ctgctgctcg gcggcccgcc cgacgttctt
      901 aagcccgtcc atggaatccg ggagccggtc gaggacctgg accatcttct cctgaacgcc
      961 ctgctgcttt tccagatgct cgcggatgcc gcgcatcagg cccagcattt cgacatagcc
     1021 ttcctgcagc gcggccaact gccggtcccg gcgggagacc ggccagtgaa tcaggcggcc
     1081 cggcttggcc ggcgcggcgg ccgcctcccc gacgtcgctc tcgggcgaga cgggcaggta
     1141 ttcctccgac ggccggcggt tccacagccg tttccaccaa gccgacagat ctgaccaggt
     1201 tcccatgatc ggcatgcccc tttttgtttc aggatagggc aagccgggcg acccggcaag
     1261 ggaattggcg atgcgcggga caaggcgccg gttccgccgg cggaaaggtt gtgcgcccgg
     1321 gcgccttgcg ctatcctttc cgcgtgaatc cgtccccgat cacctatttc atggtccact
     1381 cgccgctgca cgaggcatcc gccatgcaga gcctggccgg gcaatgcatc catcgactcg
     1441 agaccgtgtc cctccggccg gcgcccgagg gcgcgcccgc ctcccccggc gacgaagtgc
     1501 tcctgctcct gccgatcagc ggcgggacgg aaaagcagat gaaggacgtt cgcgacgcgt
     1561 gggatcgggc gggccgccgc cccgtctggg tgctggcgat tccccatcac agctccctgg
     1621 cggcctcgct ggaattcctc gcttccttgc ataacacggg gattcgcggc cgggtcttct
     1681 acttcgatcc cgcggccggc gacgacgacc cgggatttcg cgcgatagcc ggggccgtcg
     1741 aagccctgcg cgcgaaccgc atcctgcacg cctcgcggct gggcctgatc ggcatgccgt
     1801 cggactggct gctcaccagc ccgcccgacg cggaactggt ccgcacggcc tggggcccgg
     1861 aactggcgta ccggcccgtg gaggagtgcc tgcgcgccct ggacgccggg cagaccatgg
     1921 accaggcgct gaaggggctg gtatcgcgcc accgcctcgc cgcgctgacg ctgcgctgtt
     1981 tcgacctgct gtccgcgcgc gacatgaccg cctgcctgtc gctggcacgg ctgaacgacg
     2041 aggggatccc ggccggttgc gagggcgaca tccccagcgc gctcgcgatg atgtgggtcc
     2101 gcgccttgtg gaacgaaccg gcctggatgg ccaacccctc gcggctggac ctgcgcctga
     2161 accggctcac gctggcccac tgcactgttc cgttccggct gacctcggga cacaccatcc
     2221 gcacacactt cgaatcgggc cggggcgagg cgatcgccgg gcagtggacc tcggacgagg
     2281 tcaccctctt ccggatcggc ggaccgaggc tcggcgaatg gtggatcgga cagggccggg
     2341 tgatcgagcg ggggcgcgac gagaatctct gccggacgca ggtcacggtg gaattcgatc
     2401 gggccgacgc cctcccggcg ctgctggagc gcccgctcgg caatcacctg gttgtgatcc
     2461 ccggcaaccg ggcggccgcc ctcgacaccg cgctggcgat acggaagcag ttgaatccgt
     2521 gacggcgcgg ccgcggcccg ctcaccgccg gaacgtgccc atcaactggc cctgggccag
     2581 gatgtggccc tccatggccc ggaggaggtc cgccttgcgc gcgcgcgcat cgaggttcag
     2641 cgccgtgtcc agcgcgaaga ccttgaaaat gtagcggtgc ggccggcccg gcggcggcca
     2701 gggcccggca tagccgctgc ggttgtagtc attcaggccc tgccgggcgc cgctgggcaa
     2761 ctgcggcgcc gcgggaatcc cttcctccag cctgcgcgtg tccggcggca ggttgaaaag
     2821 cacccagtgc gcccagtcgc ccgcgggcgc gtccgggtcg tcgcacagga cggcgaggct
     2881 cttcgtgccc tgcggcgcgg gcccccattc caggggcggc gacacgttct ccccgccccc
     2941 ggaatacttg tgcggaatgg ccgcccccat catgaaggcg gcgctcacca cggccaactc
     3001 cagcatggct tctccttccc gcggttattt ctgctcgaac cggatccgcg cggactgcgc
     3061 gtgcgcgtcc agccgctcga cccggccgaa cgcctcgatc acgggcagca cgtcctgcag
     3121 gtccgcgcgc gtcagggaaa tgacgctgct gcggcgccgg aagctgtcca cgctcaatcc
     3181 ggagaagagc gccgccgtgc cgccggtggg cagcacgtgg ctcgggccgg cgacgaaatc
     3241 gcccgccgac tcgggggtcc atgcgccggt gaacacggcg cccgccgcgc ggaccttgga
     3301 cacccagcgg cggggttcgc gcgtcatgat ctcgagatgc tccggcgcga agcggttgca
     3361 cagctccatg ccggcgtcca gatgatcgac gacgaccagc agcgtcccct cctccaggac
     3421 ccgttccacg gcctcccggc gggacagccg ggcggtctgc cggaccagct ccttccgaac
     3481 ggactcggcc agcgcggcgg agggcgtgac gagcaacgcc ttttcgtggc cggtgccgtg
     3541 ctcggcctgg gacagcaggt ccgccgccac gtactcggca ggcgcggtat cgtcggccag
     3601 gatggcgatc tcgctggggc cggcgatcat gtccaggtcc acctcgccgt acacgaggcg
     3661 cttcgccgcg gtgacgtacg ggccgccggg gccgacgatc ttctggacct tgcggatggt
     3721 ggcggtgccg taagccatcg cgccgatggc ctggatgccg ccgaccttgt agatctccgt
     3781 ggcgccggcc aggtctaggc tgtagagcag gtagggatcg agcgcgcctt cctttcccgc
     3841 tggggaacag gccacgacct cccgcacgtc ggcgacgcgc gccagcgtga cggtcatcag
     3901 ggccgtggac gccagcgggg cggcgcccgc cggcacgtag caaccgacgc gcgcgagggg
     3961 gacgtactgc tcgcccagca ccccgccctt cggcgtggcg atggtccagt ccttccggag
     4021 cccggcctgg gcgaagcgga cgatgcggcg atgggcctcc gtggccgcgc gcttgaaatc
     4081 gacgtccacc tgcgcgatgg tggccttacg ctcctcgacg gaaacggcca ggccggccgg
     4141 ctcgaggacg gcgccgtcga actcgcggac gaagcgcaca acggcctcgt cgccctgctg
     4201 gcggatctcc tcgagcacgc gccgggcctt tttctcggcc gcgggatcga tggccggccg
     4261 ccggaggaag cggtccacgt ccggcacgga gttgccgccg gaccagcgtg tcaccttgat
     4321 atccatggta agccccgtgc gctcgctttc tatcccctct ttaccacttc gttcatgcaa
     4381 ctgcaatgcg ggcaccgcgc gagcggcagg tcgcgcgtct cgacgtacac gcggcggcag
     4441 acggcgcacc ggtagatgtt ggacgcccgc ttccgccgcg cgcgcacccg tccgtcccgc
     4501 gtgtaccagg cgccgaggcc cagcacaacg agcaaagcca tcgccatcgg aagccagagc
     4561 aaggtgaaaa tggagacggg gatcacgggg cctcctcgcc cgcgccgggc gcgcggaaaa
     4621 cgacgcggcc gctggctgcc gacgcgcccg gcttgatcct ccaattccac agcgcctttt
     4681 ccaacaggcg gttgacctcg gggcactcgg aaggcgattc caggaaaacg tgctcggcgg
     4741 cgccgcccgc gtccatctcc aggtacgccg tcgcttccca tgcacggtcg ccggtctttc
     4801 cccaacccgc gggcagaggc atatcctcga aatcatcctc ggaccagccc ggcctgaatt
     4861 ccacggacgc ggacggggcg cggggcggcc cgcggaaaac ggtggcctcc aggggcggga
     4921 gcggcacaag gaaaaggttg gcggcgacct cttcctgcag gctgtccccg cggccggcgc
     4981 ggccggtcct gccgtcgcgc tccagcatca acggcgcgga ttcccacacc ccggccgccg
     5041 gccgggcgct gaagcgctcg gccaaggccg gggcgctgaa cccggcggcg ctgggaaggg
     5101 agaacagggc cggcgaccac agcagtcggg gatcggcgga atcctccgct tcttccggcg
     5161 tcgcggcata caccagggtc ggcgcggagg ccatggccgg acgcggcgtc tccggatgcc
     5221 acgagaatcc cgcgagccag gccaggtgcc aggcgaccgc gaggaggatg cccagccccc
     5281 acgaaaccgt gtgatggcgg cgatcgggcc gcgggggcag cggcgcgggg gcccggcggg
     5341 agggcaacgc cggcagttcg gccacgcccg tgatcatgga gcctccgccg tcctctcggg
     5401 cagccgcgtg gccagcagca cctcccggaa accagcctcg gtggccatcg cgtagatgtg
     5461 gacgagcacc tggtggcgga cgcggccgtc cgcctcgatg agcagcgggg acggggtccg
     5521 gcccacggcc ttcgcgagcg cggccggcag ctccgccagg ctcacccgct cgtcctcgaa
     5581 gaagatctgt ccctcctggg taagggtcac cacgcgggtg ccgtaatgcg cgcccgcggt
     5641 gaacggggcg gcgggcagct gcatgcggat cccgggctgg aggacgaagg cggaattgac
     5701 gatatggaag agccacagga ggaggaagac attcagcaac gggacggtcg ccatcgggcc
     5761 ggtggccggc cgcagccgcg tgcgaaagcg ccggcgaagg gtgctcaacc tttcgatctc
     5821 gggctggaag gacggggaca tggccgcctc acgctcccgg tcggcgggcc ggtccggtca
     5881 ggtaggccag gatgtccacg gcggcccgct ccatgtcgag caggatggcc tccacgcggc
     5941 tgaccaggaa attgtagccc gcgtaggcgg ggattccgac ggcgaggccg gcggccgccg
     6001 tgatcagcgc ctgccacagg ccgcccagca cgtcgccggc atggatcagc ggcgccttct
     6061 gctcgatcac ctgcaggaca tggatcatgc ccatcaccgt gcccagcagc cccagcatcg
     6121 gggccacctg ggccagggtc gccaggagcg tcaggcgccg ttcgagccgc gggacctcgg
     6181 ccagcccggt ttcctcgatg gcctgcgcga tggcgtgccg ctcctcgtcg tggtgcagga
     6241 tggcggcgcg caccacgctg gccacgggcc cgggggtctc ctcgcagatg ctgacggcct
     6301 cgacgatatt gtggcggttg aggatgttga aaatgccctt gaggaaatcg ccggaccgga
     6361 tctgggcgcg gtgcagttcg tagaacttgg cgaagaacac cgcgactccg aggaaactgg
     6421 cggccaggat catccacatg accggcccgc ctttctgcat gatatccagc atcgccgctc
     6481 tcctttcggg ccggcaacgg ccccgtcaaa acaggatcca gtgctccgat tgaatctgcc
     6541 ggatgcgcag ctccgcctcg gccgccgccg gcacccccgc ctcctgcacg cgcttgtaga
     6601 ccttcaccgc ctcgcgccat ttcgactggg actccaggat cgccgcggcg ttgcggagcc
     6661 ggccggggtt cctgccgagc tgcgtggc
//
LOCUS       DAOOTK010000152         6649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7123_length_6649_cov_4.625569, whole genome shotgun sequence.
ACCESSION   DAOOTK010000152 DAOOTK010000000
VERSION     DAOOTK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7123_length_6649_cov_4.625569"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK634_12840"
     CDS             complement(<1..254)
                     /locus_tag="PK634_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOW98662.1"
                     /translation="MIPKSQSVLVHPAARLGGTLSMSGDKSISHRVAMLAALAKGTSK
                     VRNFLQSEDCLNTLHAMERLGARSFFTDDGELTIQGTGGK"
     gene            complement(258..1139)
                     /locus_tag="PK634_12845"
     CDS             complement(258..1139)
                     /locus_tag="PK634_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOW98663.1"
                     /translation="MKTVAIIGLGLMGGSLGLALKASGFGGRVRGYGRREETRAEAQR
                     RGAVDEVFGDAGVAVEGAELAVFCAPILAIPPLVSNARAHLSPGCLLTDVGSTKAYLA
                     GQIGSRLRGTTSVFIGSHPIAGSEQQGIQAARADLYRGAMIVVTPTRDTPAPALETLR
                     AFWERVGGIVRAMDPESHDQALARTSHLPHLAAALLASTVGRDDDPASWAELCGAGFR
                     DATRIAEGSPEVWHDIIRTNAGAVARELRAFAGEVDQLSRLIEGEQFDSMKRFLEKAR
                     GRRRTLARGRAGEDAQG"
     gene            complement(1136..2170)
                     /gene="aroF"
                     /locus_tag="PK634_12850"
     CDS             complement(1136..2170)
                     /gene="aroF"
                     /locus_tag="PK634_12850"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOW98664.1"
                     /translation="MIIVLRRDATEEQVGHLVATIRGWGLTTHVSRGTERTIIGVIGD
                     ERAIRSKPLEAIPGVESVMAVLKPYKLASLEFQPERTRIRIPAVKPGGAPVEIGGPSV
                     VVVAGPCSVENRDMLFDMARVIRGAGAKLLRAGAFKPRTSPYAFQGLGVEGLRYLAEV
                     RAEVGIPIITEVMDTRDVEMIAEVADILQIGARNMQNFNLLKAVGRCRKPVLLKRALA
                     GTIEELLMSAEYILSEGNREVIVCERGIRTFEKATRNTLDISAVPVIRRESHLPVFVD
                     PSHGTGVWPLVAPMALAAVAAGADGIMVEVHPNPEQALSDGPQALLPKTFEKMMKDLQ
                     AVAQAVGRTL"
     gene            complement(2167..3276)
                     /gene="hisC"
                     /locus_tag="PK634_12855"
     CDS             complement(2167..3276)
                     /gene="hisC"
                     /locus_tag="PK634_12855"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOW98665.1"
                     /translation="MKHIANPWVHSLGTYEPGRPIEEVARELGFENIEEVVKIASNEN
                     SLGPSPKAVEAMIRSAPRMHLYPDGGAFYLRRALAERLRVHMDEILVGNGSNEIIELM
                     GHVFLQPGSNIVMADRAFVVYKLITAAMQAQTISVPMTNYTHDLDAMLEAITPETRIV
                     FVANPNNPTGTMVNGAELDRFMARVPDHVVVALDEAYIELLPPSQQPDTLKYVRQGRH
                     VYVLRTFSKTYGLAGLRVGYAVASRHGIELLHRVRQPFNVNAMALEAARAALEDEAYV
                     ERTRKMVREGLEQLAGAFDEMKLEYVPSVANFILVKVGRGREVFEALQRRKVIVRPMD
                     GYGLPDHVRVTVGLKQENERFLQGLRAILAERGGA"
     gene            complement(3290..4363)
                     /gene="pheA"
                     /locus_tag="PK634_12860"
     CDS             complement(3290..4363)
                     /gene="pheA"
                     /locus_tag="PK634_12860"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOW98666.1"
                     /translation="MSLEELRKKIDGLDAELVRLLNERTRLALEVGRAKEAASQDVYV
                     PAREKAVLDRVDALNQGPLPADAMRAIYREIMSASLALERKVVIAYLGPPATFTHQAA
                     RSRFGGSVQYAPCETIGEVFAAVEKKAADYGVVPIENSTDGAVTHTLDQFTDTPLKIC
                     AEIYLPISHNLLAKCPKGEIKRLYSKIEVFGQCRRWLHENMPGVELISSSSTARAAET
                     AAREEGSGALASALTADLYGLDLLEQDIQDLGGNTTRFLVIGPRYGEATGEDKTSLLF
                     AVKHRVGALYDALSAFKRYSINLTKIESRPSKTKAWEYYFFVDVEGHADDPPLQKALA
                     DLGEHCTLMTVLGAYPRASGMDS"
     gene            complement(4360..5169)
                     /gene="scpB"
                     /locus_tag="PK634_12865"
     CDS             complement(4360..5169)
                     /gene="scpB"
                     /locus_tag="PK634_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOW98667.1"
                     /translation="MSDVEILPELKEIIGAMLFVARQPLTLADLKRVMGQVAETQGGI
                     TKDYARASEADLAAALQQLKTDLVEKKVGLEINEVANGFRMENNALCGPWLRHLLEKG
                     RPSRLSRPALETLAIIAYRQPCTRADIEKVRGVAVDQIIRNLLEMQLIRITGRSELPG
                     RPWLFGTTQRFLEHFGLRDVDDLPSVQELRRLETEQIRKNAPAGAGAPPEEAGGGSEA
                     NAAGSEAPEGPADAGEPPAAEEELEEDEEEEYDEDEGEDEEDGEGDEEDAP"
     gene            complement(5186..5920)
                     /locus_tag="PK634_12870"
     CDS             complement(5186..5920)
                     /locus_tag="PK634_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOW98668.1"
                     /translation="MPPQEEYKVRLDVFEGPLDLLLYLIKKEELDIYDIPIERITTQY
                     MQYLDLMRMLDLNIAGDFIVMAATLMMIKSRMLLPVEERTAMEEEEEDDPRWDLVRQL
                     VEYKKFKDAALHLEALEEQRLDVFAREGEHVQLGPAPEIALHDVSLFDLITAFGDVLK
                     RVKTEELKEIFSERFTVAEKMEQIVRRLQAEEKIAFGSLFAQMASRHEIVCTFLALLE
                     LIRLKQLVARQESSFGEIVVLRAEGL"
     gene            complement(5939..>6649)
                     /gene="trpS"
                     /locus_tag="PK634_12875"
     CDS             complement(5939..>6649)
                     /gene="trpS"
                     /locus_tag="PK634_12875"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOW98669.1"
                     /translation="VTELTWLLSTVTPMGLLERCHSYKDKVAKGLPATHGLFAYPVLM
                     AADILIMQSQVVPVGRDQKQHVEVTRDIAIKFNNLYGEVFTLPAPSIRDEVAVVPGVD
                     GQKMSKSYNNTVEIFGDPKESRAQIMRIVTDSKTVADPKDPDQCNVFALYQLFASNAQ
                     RDEMAARYRAGGLGYGEVKKRLFEMFDAHFAPLRQRRAELQKNMDHVEAVLRSGAERA
                     AAVARDTLRRARQAVGLA"
BASE COUNT          952 a         2347 c         2173 g         1177 t
ORIGIN      
        1 aacttgccgc cggttccctg gatggtcagt tcgccgtcgt cggtgaaaaa ggaccgcgcg
       61 ccgagccgct ccatcgcgtg cagggtgttc aggcagtcct cgctctggag gaaattccgc
      121 accttcgacg tccccttggc gagcgcggcc agcatggcga cgcggtggga gatgctcttg
      181 tcgccggaca tggacagcgt tccgccaagc cgggccgccg ggtgaaccag gacggactgc
      241 gattttggaa tcatatgtca gccttgcgca tcttcgccgg cccgcccgcg ggccagggtc
      301 ctccgccgcc cgcgcgcttt ttccaggaac cttttcatgc tgtcgaactg ctccccttca
      361 atcaagcgcg aaagttggtc cacctcgccg gcgaaggccc gcagttcccg ggccacggcg
      421 ccggcattgg tgcggatgat gtcgtgccag acctccggcg aaccttcggc aatgcgggtc
      481 gcgtcgcgga atcccgcgcc gcatagttcc gcccaggagg ccgggtcatc gtcgcgtccc
      541 acggtcgacg ccagcagcgc ggcggccaga tgcggcaggt ggcttgtccg ggccagggcc
      601 tggtcgtgcg attcgggatc catggcgcgg acgatgccgc ccacgcgctc ccagaaggcc
      661 cgaagggtct cgagggccgg cgcaggggta tcgcgcgtcg gcgtcaccac gatcatggcg
      721 ccgcggtaga ggtccgcgcg ggcggcctgg attccctgct gctccgaccc ggcgatgggg
      781 tggctgccga tgaaaacgga ggtcgtgccc cgcagccggg agccgatctg gccggccagg
      841 taggccttcg tgctgccgac atccgtcagc aggcagccgg gcgacaggtg cgcccgggca
      901 ttgctgacca gcggcgggat cgccaggatc ggcgcgcaga acaccgccag ttccgcccct
      961 tccaccgcca cgccggcgtc gccgaagact tcatcgacgg cgccccggcg ctgcgcttcc
     1021 gcgcgggttt cctcgcggcg cccgtaaccg cgcacccggc ccccgaaccc ggaggccttc
     1081 aacgcaaggc ccagcgagcc gcccatcagc cccagcccta tgatcgccac cgtcttcaca
     1141 gggtgcggcc cacggcctgc gcgacggcct gcaggtcctt catcatcttc tcgaaggtct
     1201 tggggaggag ggcctgcggc ccgtcggaca gcgcctgctc ggggttcggg tgcacctcga
     1261 ccatgatccc gtccgccccg gcggcgacgg cggccagcgc catcggcgcc accagcggcc
     1321 atacgcccgt gccgtgactc gggtccacga agaccggcag gtggctctcg cggcggatca
     1381 ccgggacggc gctgatgtcc agcgtgttcc gcgtcgcctt ctcgaaggtc cggatgccgc
     1441 gctcgcacac gatcacctcg cggttgccct cgctcaagat gtactccgcg ctcatcagca
     1501 gctcctcgat tgtcccggcc agcgcgcgct tcagcagcac gggcttgcgg cagcggccga
     1561 cggccttgag caggttgaag ttctgcatgt tccgcgcccc gatctggagg atgtccgcga
     1621 cctcggcgat catctcgacg tcgcgcgtgt ccatgacctc ggtgatgatg gggatgccga
     1681 cctccgcccg gacctcggcc aggtagcgaa ggccctcgac gccgagcccc tggaaggcat
     1741 agggcgaggt gcggggcttg aacgcgccgg cgcggagcag cttcgccccc gcgccgcgga
     1801 tcacccgggc catgtcgaag agcatgtcgc ggttctccac cgagcaggga ccggcgacca
     1861 cgacgaccga cgggccgccg atctccaccg gcgcgccgcc cggcttgacc gcggggatcc
     1921 ggatgcgcgt gcgctcgggc tggaactcga ggctggccag cttgtacggc ttgagcacgg
     1981 ccatcacgga ctcgacgccc gggatcgcct ccagcggctt ggagcggatg gcgcgctcgt
     2041 cgccgatcac gccgatgatg gtccgctccg tcccgcgact gacgtgcgtc gtcaggcccc
     2101 agccccggat cgtcgccacg aggtggccga cctgctcttc cgtggcgtcg cgccggagaa
     2161 cgatgatcat gccccgcccc tttcggccag gatcgcccgc aggccctgga ggaaccgctc
     2221 gttctcctgc ttcaagccca ccgtcacccg cacgtggtcg ggcagcccgt agccgtccat
     2281 cggccggacg atcaccttgc ggcgctgcag cgcctcgaac acctcgcgcc cgcggccgac
     2341 cttgaccagg atgaaattgg cgacggacgg cacgtattcc agcttcatct cgtcgaaggc
     2401 gcccgcgagc tgctccaggc cctcccggac cattttccgc gtgcgctcga catacgcctc
     2461 gtcctccagc gccgcgcgcg cggcctccag ggccatggca ttgacgttga agggctggcg
     2521 gactcgatgc agcagctcga tgccgtgcct cgacgcgacc gcgtatccga cgcgcaggcc
     2581 ggccaggccg tacgtcttcg aaaaggtccg cagcacgtac acgtggcggc cctgccggac
     2641 gtatttcagc gtgtccggct gctgcgacgg gggcagcagc tcgatgtagg cctcgtccaa
     2701 ggcgacgacc acgtggtccg gaacccgcgc catgaaccgg tccagttcgg cgccgttcac
     2761 catcgtcccc gtcgggttgt tgggattggc gacgaagacg atccgggtct ccggcgtgat
     2821 ggcctccagc atggcgtcca ggtcgtgcgt gtagttcgtc atgggcacgc tgatcgtttg
     2881 cgcctgcatc gccgcggtga tgagcttgta gaccacgaag gcccggtcgg ccatgacgat
     2941 gttgcttccg ggctgcagga agacatgtcc catcagctcg atgatctcgt tgctcccgtt
     3001 gccgacgagg atctcgtcca tgtgcacgcg gagccgctcg gccagggcgc ggcggaggta
     3061 gaaggcgccg ccgtcgggat aaaggtgcat ccggggcgcg gagcggatca tggcctccac
     3121 ggccttcggc gacgggccca gcgagttctc gttggaggcg atcttgacga cctcctcgat
     3181 gttctcgaag ccgagctccc gcgcgacctc ctcgatcggg cggccgggct cgtacgtgcc
     3241 gaggctgtgc acccaggggt tggcgatgtg tttcatggca gtcgtgctcc tacgaatcca
     3301 tgccggacgc ccgcgggtac gcgccgagca ccgtcatcag cgtgcagtgc tcgccgaggt
     3361 cggccagcgc cttctgcagc ggcgggtcgt ccgcgtgccc ctcgacgtcc acgaagaaat
     3421 agtactccca cgccttcgtc ttgctcgggc ggctctcgat cttggtcagg ttgatgctgt
     3481 accgcttgaa cgccgacagg gcgtcgtaga gcgcgcccac gcggtgcttg acggcgaaaa
     3541 gcagcgaggt cttgtcttcg cccgtcgcct ctccgtagcg cgggccgatg accaggaagc
     3601 gcgtcgtgtt gccgccgagg tcctggatgt cctgctccag gaggtccagc ccgtacaggt
     3661 ccgccgtcag ggcgctggcg agggcgccgc tcccctcctc gcgggccgcg gtctccgccg
     3721 cgcgcgcggt gctggacgag gagatcagct cgacgccggg catattctcg tgcagccagc
     3781 ggcggcactg cccgaacacc tcgatcttgc tgtacaaccg cttgatttcg cccttcgggc
     3841 acttcgccag caggttgtgc gagatgggca ggtagatctc ggcgcagatc ttcagcggcg
     3901 tgtccgtgaa ctggtccagc gtatgcgtca ccgccccgtc ggtcgaattc tcgatcggca
     3961 cgaccccgta gtcggccgcc tttttctcca cggcggcgaa gacctcgccg atggtctcgc
     4021 agggggcgta ctgaacgctg ccgccgaagc ggctgcgcgc ggcctggtgc gtgaacgtcg
     4081 ccggcggacc caggtaggca atcacgacct tgcgctccaa ggccagcgag gcggacatga
     4141 tctcgcggta gatggcccgc atggcatccg ccgggagcgg gccctggttc agggcgtcca
     4201 cccggtcgag gacggccttc tcgcgggccg ggacatacac gtcctgcgac gccgcttcct
     4261 tggcacggcc gacttccagg gccaggcgcg tccgctcgtt cagcaggcgg accagttcgg
     4321 cgtccagccc gtcaatcttt ttccggagtt cttccaggct catggcgcat cctcttcatc
     4381 cccctcgcca tcctcttcgt cctcgccttc gtcctcgtcg tattcctctt cctcgtcctc
     4441 ttccagctct tcttccgccg cgggcggttc tccggcgtcc gccggcccct cgggggcctc
     4501 gctacccgcg gcattcgctt ccgacccgcc gcccgcttcc tccggcggcg ccccggctcc
     4561 cgccggggcg tttttccgga tttgctcggt ttccagccgg cgcagttcct ggacgctcgg
     4621 caggtcgtcc acgtcgcgca ggccgaagtg ctcgaggaaa cgctgcgtcg tgccgaacag
     4681 ccacggccgg cccggcaact cgctgcgccc ggtgatccgg atcaactgca tttccaggag
     4741 gttccggatg atctggtcca ccgcgacgcc gcggaccttc tcgatgtcgg cccgcgtgca
     4801 gggctggcgg taggcgatga tcgccagcgt ctccagcgcc gggcgggaca ggcgcgaggg
     4861 ccgtcccttt tccagcaggt gccgcagcca cggcccgcaa agcgcgttgt tctccatccg
     4921 gaacccgttg gccacctcgt tgatctcgag gcccaccttc ttctccacca gatccgtctt
     4981 cagctgctgg agcgccgcgg ccaggtcggc ctcggacgcc ctggcgtagt ccttcgtgat
     5041 cccgccctgc gtctcggcga cctgccccat gacgcgcttc aggtcggcca gcgtcagcgg
     5101 ctggcgcgcc acgaagagca tcgccccgat gatttccttc agctcgggaa gtatttccac
     5161 atcgctcatg gcgcctcgac ctttatcaca agccctcggc ccgcaacaca acgatttctc
     5221 cgaacgacga ctcctgccgg gccaccagct gcttgagccg gatcagctcc agcagcgcca
     5281 ggaaggtgca gacgatctcg tgccgggagg ccatctgcgc gaacaggctc ccgaacgcga
     5341 tcttttcctc ggcctgcagc cgccggacga tctgctccat cttctccgcc acggtgaatc
     5401 gctcggagaa gatctccttc agctcctccg tcttcacccg tttcagcacg tcgccgaacg
     5461 ccgtgatgag gtcgaacagg ctgacgtcgt gcagggcgat ctcgggggcc ggcccgagct
     5521 ggacgtgttc gccctcgcgc gcgaagacat ccagccgctg ctcctccagg gcttccaggt
     5581 gcagggcggc gtccttgaac ttcttgtatt ccacgagctg gcggaccagg tcccagcggg
     5641 gatcgtcctc ctcttcctcc tccatcgcgg tccgctcctc gacgggcagg agcatgcggc
     5701 tcttgatcat catgagcgtg gcggccatga cgatgaagtc gccggcgatg ttcaggtcca
     5761 gcatgcgcat caggtccagg tactgcatgt actgggtcgt gatgcgctcg atgggaatat
     5821 cgtagatgtc cagctcttcc ttcttaatga ggtagagcag gagatcgaga ggcccctcga
     5881 agacatccag ccggaccttg tattcttcct gcggaggcat gcgcgtccct ccgggcggct
     5941 aggccagccc cacggcctgg cgggcgcggc gcagcgtgtc ccgcgccacg gcggcggcgc
     6001 gctccgcgcc gctccgcagg acggcctcga cgtggtccat gttcttctgc agctccgccc
     6061 gccgttgccg cagcggggcg aagtgcgcgt cgaacatctc gaagagccgt ttcttcacct
     6121 cgccgtatcc caagccgccc gcccggtagc gcgccgccat ctcgtcgcgc tgggcgttgg
     6181 aggcgaagag ctggtagagg gcaaagacgt tgcattggtc gggatccttc gggtcggcca
     6241 cggtcttgct gtcggtgacg atccgcatga tctgcgcccg ggattccttc gggtcgccga
     6301 agatctccac ggtgttgttg taggacttcg acatcttctg cccatccacg cccggcacca
     6361 ccgccacctc gtcgcggatg gagggggccg ggagggtgaa gacctcgccg tagagattgt
     6421 tgaacttgat ggcgatgtcc cgggtgacct cgacgtgctg cttctggtcg cggccgacgg
     6481 ggaccacctg ggactgcatg atcaggatgt ccgcggccat cagcaccgga taggcgaaca
     6541 gcccgtgcgt cgccggcagg cccttggcga ccttgtcctt gtaggagtgg cagcgctcga
     6601 ggaggcccat gggggtcacc gtggacagca gccaggtcag ctccgtgac
//
LOCUS       DAOOTK010000153         6597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7190_length_6597_cov_5.442678, whole genome shotgun sequence.
ACCESSION   DAOOTK010000153 DAOOTK010000000
VERSION     DAOOTK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7190_length_6597_cov_5.442678"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK634_12880"
     CDS             <1..1300
                     /locus_tag="PK634_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOW98670.1"
                     /translation="ADAVTNARVGHAERRQAAEHMVERQKPLQARIQEIEELIRTRSE
                     DLVSYRARIGDLERQLAETKTRLQPMEEEVARQAAHLDAARKRREEALAAVGVWDKRL
                     HEQRANMDDVRNRKGQVDVELAEQRVRRQNLVERAMAEYHITLDQLAAAPEPAWENGQ
                     RPDREEMETQIAELRAKLESMGPVNLVAIEEYKQHEERHDFLTRQQDDLVNAKQQLLD
                     FIRRINQTTTEMFQQTFNRVNANFQDMFQKLFGGGTAKLVLVDEGDVLESGIEIIARP
                     PGKKLQTVSLLSGGERTMTAVALLFSLYMVKPSPFCLLDELDAALDEANIGRFIKVVQ
                     GFLESSQFIVITHNRQTIGAADILYGVTMEHQGVSKIVSVKFSHHEKAKPPAKSEPAP
                     AKEPPPAPAAAPEPVSQPAAASTPPEAPAGEAPAAEPPPA"
     gene            complement(1320..3122)
                     /gene="dnaG"
                     /locus_tag="PK634_12885"
     CDS             complement(1320..3122)
                     /gene="dnaG"
                     /locus_tag="PK634_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOW98671.1"
                     /translation="MGQILSKALLEQIRAANDIVEVVGAYLPLKRAGSSFKGLCPFHK
                     EKTPSFHANPQRQSFHCFGCGAGGDVFRFIMQYDGVDFVSAVRLLARRAGIAVELTED
                     DRQEGPNKDLLLKLLEDVTMVYHHALQKLPAAEAARAYLRKRDLGDAVVRDYLLGYAP
                     EGWDTLVKWGQKKNYSPALLEAAGLLSRSDSEEGRVYDRFRHRLMFPVRDEIGRIVGF
                     SGRVLADDPKAAKYVNTPETPVFHKGKLLYALDKARRAIMDSRTALLCEGQIDVIRCH
                     LGGFQNAVAAQGTALTEQHAQVLKRYADSVIVVLDADPAGQKASLRSAELLLAAGLSV
                     SIASLPPDEDPDSLIRAKGPAAFQAVLDKARPLLDFQVALLRQGEGFSGEAGLMRAAR
                     AVLETIAKAPTAVQRDHLLRQAARELGLTENALRQDLNKLTRTSGPARVQAEGEPEAK
                     VQHPLEEVTVLELSVRHPEVVLLFRRYLPADAMTDPTCRRLYELVLVGEENPMGALGP
                     DSPEAARLLAQVEAAPSRLVGEDLTAEHPAQDLILRLRRKQMERRRVELRQKLSSAPE
                     SERQALDLECKQLTLDIKSLQQGWDRALPILELD"
     gene            complement(3130..3735)
                     /locus_tag="PK634_12890"
     CDS             complement(3130..3735)
                     /locus_tag="PK634_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOW98672.1"
                     /translation="MENLPQFNLVDILVMIYIVLGVVRGFLNGLSGELARLLSVVVAV
                     AAGIHFYEPMGGYLLEHTRLGEWDPKTAYMVSFGVLLLGGWLAMRLLRVVLRHLMEFT
                     FRGRIEKVGGALAGFLRYSVEAGAIILLLGLVPHEAMRRHFVDESFFGRPLAQYVLPA
                     YEKLAEKYPALRIPVRQEAGDVEKEPADAEPPAEAAPEEGE"
     gene            3848..5143
                     /gene="pcnB"
                     /locus_tag="PK634_12895"
     CDS             3848..5143
                     /gene="pcnB"
                     /locus_tag="PK634_12895"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOW98673.1"
                     /translation="MQPLIIDRSQHTVSRKNIAPEALRVLYRLHEAGFTAYLAGGGVR
                     DLLLGRTPKDFDIATSAHPNQVKRLFRNCRLIGRRFRLAHVFFPDIIIEVSTFRAHVP
                     ADSAQRVTTKEGMIVRDNVFGTPPEDALRRDFTVNALFYDIADYSLVDYVGGLKDLDA
                     RLLRVIGDPRVRFQEDPVRMLRAVRFAASLDFTIEETARQAILDMKEHLAQASRERLH
                     EEMLKIFFCGRAEAVLQGLFDTDLFAVLFPEVAAWLDGAGGEAGRDWMRRALRQVDKW
                     KAAGLRPGESLLWALLFGPYFEARSADPGLANLSRTAARARTAHDQLLNPSHRVQIPR
                     NVARGTADILAAQAVFPRTQGKGPRRFAARHVFKDAFVFFKFSARARGENEELVKWWS
                     DFLGGLRDPAPEPPAPAPEAPQRGGVASPSDETSGTSII"
     gene            complement(5086..>6597)
                     /locus_tag="PK634_12900"
     CDS             complement(5086..>6597)
                     /locus_tag="PK634_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOW98674.1"
                     /translation="VGEGRSSVVWAARTGPDAPPVALKWLRTDEVPDESFRRRAMEEA
                     RASSRLHHPGIVDLKDVGEIDGGLYYVMEFVDGCTLAELLARKGRLQPKHAMQIAEGI
                     ALALTYAWDRERLIHHDLCPGNIMLERDGTVRVADMGILPPGGVAYRARLSYAAPEQA
                     DGSASVDYRANIYSAGALLYEMMTGRKPFRELTGVEALDRQVPEQLPDPQELNREVTS
                     GVGWLVEKSMIRDPANRYRTWAEWLADLQEVAKGGWPLSELPVSGQSLVLRSALRFPP
                     PVNIVEPAPTAKKFLRSRKQAPAGKRVLVPKAPSATVSAPARSRAASELARSLSAFLV
                     LGAITAVAYAGFWLHGRWKAGAFEQETPPAEPAVLPQPDIPEDSRKMSFDGTVRPPEP
                     APTPAVEPKPAGAEEVITWDHPIFVRGARSFNEAVALYQKYIQDRKDPALLKEIEKKC
                     REAIRDFESCRNEAPPEVGMAEILTQAYRLLSDCRQMMLVPDVSSDGEATPPR"
BASE COUNT         1038 a         2289 c         2315 g          955 t
ORIGIN      
        1 ggcggacgcg gtcacgaacg cccgggtcgg ccacgccgag cggcggcagg ccgccgagca
       61 catggtcgag cgccagaaac ctctgcaggc ccgcatccag gagatcgagg aactcatccg
      121 cacgcggtcc gaggacctcg tgtcgtaccg cgcgcgcatc ggcgacctgg agcggcagct
      181 ggcggagacg aagacccgcc tgcagccgat ggaggaggaa gtcgcccgcc aggccgcgca
      241 cctggacgcc gcgcggaagc gccgcgagga ggccctggcc gcggtgggcg tctgggacaa
      301 gcgcctgcac gagcagcgcg cgaacatgga cgacgtgagg aaccgcaagg gacaggtgga
      361 cgtcgaactc gccgagcagc gcgtgcgccg ccagaacctc gtcgagcgcg ccatggccga
      421 gtatcacatc acgctggacc agcttgccgc cgcgccggag cccgcctggg aaaacggcca
      481 gcggcccgac cgcgaggaga tggagacgca gatcgccgag ctgcgcgcca agctggaatc
      541 catggggccg gtcaacctcg tcgcgatcga ggagtacaag cagcacgagg agcggcacga
      601 cttcctgacg cggcagcagg acgacctggt caacgccaag cagcagttgc tggatttcat
      661 ccggcgcatc aaccagacca cgaccgagat gttccagcag accttcaacc gggtgaacgc
      721 caacttccag gacatgttcc agaagctgtt cggcggcggc acggccaagc tggtgctcgt
      781 cgacgaaggc gacgtgctcg agtcgggcat cgagatcatc gcccggccgc cgggcaagaa
      841 gctgcagacc gtctccctgc tctccggcgg cgagcgcacg atgacggccg tcgcgctgct
      901 gttctccctc tacatggtca agccgagccc cttctgcctg ctggacgagc tcgacgcggc
      961 cctcgacgag gccaacatcg gccggttcat caaggtggtg cagggattcc tggaaagctc
     1021 gcagttcatc gtcatcacgc acaaccggca gaccatcggc gcggcggaca tcctctacgg
     1081 cgtgacgatg gagcaccagg gcgtctccaa gatcgtctcg gtgaaattct cgcaccacga
     1141 gaaggccaag ccgccggcga agagcgaacc ggccccggcc aaggaaccgc cccctgcgcc
     1201 agccgcggcg ccggaacccg tttcgcaacc ggccgcggca tccactccgc cggaagcgcc
     1261 cgcgggcgag gcgcccgcgg cagagccgcc gccggcctga tcagaacccc ggcgggcgat
     1321 cagtccagtt cgagaatggg cagggcgcgg tcccacccct gctgcagcga cttgatgtcg
     1381 agcgtcagct gcttgcactc cagatcgagc gcctggcgct cgctctcggg agcggaactc
     1441 aacttttgtc gcagttcgac ccggcgccgc tccatctgct tccgccgcag ccggaggatg
     1501 agatcctgcg cgggatgctc cgcagtcagg tcttccccca ccagccgcga aggcgcggcc
     1561 tcgacctggg ccaggaggcg cgccgcctcc gggctgtccg ggcccagcgc gcccatcggg
     1621 ttctcttcgc caaccagcac aagctcgtac aggcgccggc aggtcggatc ggtcatcgcg
     1681 tccgcgggca ggtaacggcg gaacagcagc accacctcgg gatggcgcac ggacaactcc
     1741 agcacggtca cttcctccag gggatgctga acttttgcct cgggctcgcc ttcggcctgg
     1801 acccgggcgg ggccggaagt ccgggtcagc ttgttcaggt cctgccgcag ggcgttctcc
     1861 gtcaagccca gctcccgggc ggcctggcgg agaagatggt cccgctgcac ggcggtcggg
     1921 gccttggcga tcgtctccag cacggcgcgg gcggcccgca taaggccggc ctccccggag
     1981 aacccctcgc cctgccgcag cagcgcgacc tggaaatcca gcaacggccg ggccttgtcc
     2041 aacaccgcct ggaaggccgc gggccccttg gcgcgaatca gggagtccgg gtcctcgtcg
     2101 ggcggcaggg aggcgatgct gacgctcaag cccgcggcga gcaggagttc ggccgagcgc
     2161 agggaggcct tctggccggc cgggtcggca tccaggacca cgatgaccga atcggcatag
     2221 cgcttgagga cctgggcgtg ctgctcggtc agcgcggtgc cctgggcggc gaccgcgttc
     2281 tggaatccgc cgaggtggca gcggatgaca tcgatctgcc cctcgcagag cagggcggtc
     2341 cggctgtcca tgatcgcgcg gcgcgccttg tcgagggcgt acagcagctt gcccttgtgg
     2401 aagaccgggg tttccggcgt attgacgtac ttggcggcct tggggtcgtc ggcaagcacg
     2461 cggccgctga acccgacgat gcgcccgatc tcgtcgcgga cggggaacat caggcggtgg
     2521 cggaaccggt cgtagacgcg gccctcctcg gagtccgacc ggctcaacaa gcccgccgcc
     2581 tccagcagcg cgggcgagta gttcttcttc tggccccact tgaccagcgt gtcccagccc
     2641 tcgggggcgt agccgagcag gtagtcgcgc acgacggcat cgcccaggtc gcgtttacga
     2701 agatacgccc gcgccgcctc ggcggccggc agcttctgca gcgcgtggtg gtagaccatc
     2761 gtcacgtcct cgagcagctt gagcaggagg tccttgttcg ggccctcctg ccggtcgtcc
     2821 tcggtcagtt cgacggcgat cccggcgcgc cgcgcgagca ggcggacggc cgacacgaaa
     2881 tcgacgccgt cgtactgcat gatgaaccgg aacacgtcgc cgcccgcgcc gcacccgaag
     2941 cagtggaagg actgccgctg cgggttggcg tggaaggacg gggtcttctc cttgtggaac
     3001 gggcagagcc ccttgaacga cgagcccgcg cgcttgaggg gcaggtaggc gccgacgacc
     3061 tcgacgatgt cgttcgcggc gcggatctgc tccagcaggg ccttggaaag gatctgtccc
     3121 atggcgcggc tactcgccct cctccggcgc ggcctccgcg ggcggctccg cgtccgccgg
     3181 ttccttctcc acgtccccgg cctcctgccg gacggggatc cggagggcgg gatatttctc
     3241 cgccagcttc tcgtacgcgg gcaggacata ctgggccagc gggcggccga agaaggactc
     3301 gtccacgaag tgccggcgca tggcctcgtg ggggacgagg ccgagcagga ggatgatcgc
     3361 gccggcctcc acggagtagc gcaggaaccc ggccagcgcc ccgccgacct tctcgatgcg
     3421 gccgcggaac gtgaactcca tgaggtgccg cagcacgacg cgcagcagcc gcatggccag
     3481 ccagccgccg agcagcagca cgccgaagga caccatgtag gccgtcttcg ggtcccactc
     3541 ccccaggcgg gtgtgctcga gcaggtagcc gcccatcggc tcgtagaagt ggatgcccgc
     3601 cgcgacggcg acgaccacgc tcaacagccg ggccagctcg ccggagaggc cgttcaggaa
     3661 gccgcgcacg acgcccagga cgatgtaaat catcaccaga atatccacca ggttgaattg
     3721 tggcaggttt tccattatga atctcccgcg cttcggcctc cgggcgcgac ccgtatccgg
     3781 gaccagtgta gggagatgga ggccgcgggc aagccggttc tccgggagcc tgacaaaaaa
     3841 tggggacatg cagccgctta tcatagatcg ttcgcagcac acggtcagcc ggaagaacat
     3901 cgcgccggag gcgctccggg tgctctaccg cctgcacgag gcgggcttca cggcctacct
     3961 ggccggcggc ggcgtgcgcg acctcctgct gggccgcacg cccaaggact tcgacatcgc
     4021 gaccagcgcc cacccgaacc aggtcaagcg cctgttccgc aactgccggc tcatcggccg
     4081 gcgcttccgg ctggcccacg tcttcttccc ggacatcatc atcgaggtct ccaccttccg
     4141 ggcgcacgtg ccggcggata gcgcgcagcg ggtgacgacg aaggagggga tgatcgtgcg
     4201 ggacaacgtg ttcggcacgc cccccgagga cgccctgcgg cgcgacttca cggtgaacgc
     4261 cctgttctac gacatcgcgg attactcgct cgtggactac gtcggcggcc tgaaggatct
     4321 cgacgcccgg ctgctgcggg tgatcggcga cccgcgggtg cgtttccagg aggaccccgt
     4381 gcggatgctg cgcgccgtcc gctttgccgc gtcgctggat ttcacgatcg aggaaaccgc
     4441 gcggcaggcc atcctcgaca tgaaggagca cctggcccag gcctcccggg agcggctgca
     4501 cgaggagatg ctcaagatct tcttctgcgg ccgcgcggag gccgtgctgc agggcctgtt
     4561 cgacacggac ctttttgccg tcctgttccc ggaagtggcg gcgtggctgg acggggccgg
     4621 cggcgaggcg ggccgcgact ggatgcggcg ggcgctccgg caggtggaca agtggaaggc
     4681 cgcgggcctg cggcccgggg aatccctgct ctgggcgctc ctgttcggcc cctacttcga
     4741 ggcccgctcc gccgatcccg ggctggccaa cctgtcccgc accgcggcgc gggcccgcac
     4801 ggcgcacgac caactgctga acccgtccca ccgcgtgcag atcccgcgga acgtcgcgcg
     4861 gggaacggcc gatatcctcg cggcgcaggc ggtctttccc cggacgcagg gcaaggggcc
     4921 gcgccggttc gccgcgcggc acgtgttcaa ggacgcgttc gtcttcttca agttctccgc
     4981 gcgggcacgc ggggagaacg aggaactggt gaagtggtgg agcgacttcc tcggcggcct
     5041 gcgggatccc gcgccggaac cgccggcgcc cgccccggag gcgcctcaac gcggcggcgt
     5101 ggcctcgccg tccgacgaga cgtcggggac cagcatcatc tgacggcagt cggagagcag
     5161 gcggtaggcc tgcgtcagga tctccgccat cccgacctcc ggcggggcct cgttacggca
     5221 ggattcaaaa tcgcggatcg cctcgcggca tttcttctcg atctccttca ggagggccgg
     5281 gtccttgcgg tcctggatat acttctggta cagggcgacg gcctcgttga agctgcgcgc
     5341 gccgcgcaca aagatgggat ggtcccaggt gatcacttcc tccgcgccgg ccggcttggg
     5401 ctcgacggca ggcgtgggcg cgggttccgg gggccggacg gtcccgtcga acgacatctt
     5461 gcggctgtcc tcggggatgt cgggctgcgg aaggacggcc ggttccgcgg gcggcgtttc
     5521 ctgctcgaag gcgccggcct tccagcggcc gtggagccag aacccggcgt aggccacggc
     5581 cgtgatcgcg cccaacacca ggaaagcgga cagggagcgg gccagttccg aggccgcgcg
     5641 ggagcgggcc ggcgcgctga ccgtcgcgga gggggccttc ggcacgagga cgcgtttgcc
     5701 ggccggcgcc tgcttgcggc tccgcaggaa cttcttcgcc gtcggggccg gctcgacgat
     5761 gttgaccggc ggcgggaacc gaagcgcgct gcgcaggacc aggctctggc ccgagaccgg
     5821 cagttcgctc aacggccagc cgcccttggc gacttcctgc aggtccgcca gccactcggc
     5881 ccaggtccgg taccggttgg ccgggtcgcg gatcatggat ttctcgacca gccaacccac
     5941 gcccgacgtg acttcccggt tgagctcctg cggatcgggc agttgctccg gcacctgccg
     6001 gtcgagggcc tccacgcccg tgagctcgcg gaacggcttg cggccggtca tcatctcgta
     6061 caacaaggcg ccggcggaat aaatgttggc gcggtaatcc acgctggcgg agccgtccgc
     6121 ctgctcgggg gcggcgtagg agagccgcgc ccggtaggct acgccgccgg gcggcaggat
     6181 gcccatgtcc gcgacgcgga cggtgccgtc ccgctcgagc atgatgttgc cggggcagag
     6241 gtcgtggtgg atcaggcgct cgcggtccca ggcataggtc agggcgaggg cgatgccctc
     6301 ggcgatctgc atggcgtgct tgggctgcag ccggcccttg cgggccagca gttcggccag
     6361 cgtgcagccg tcgacgaatt ccatgacgta gtagagcccg ccgtcgatct cgccgacgtc
     6421 cttgaggtcc acgatgccgg ggtgatgcag ccggctggag gcgcgcgcct cctccatcgc
     6481 gcggcgccgg aagctctcgt cgggcacctc gtccgtgcgc agccacttca gcgccaccgg
     6541 cggcgcatcc ggcccggtcc gcgcggccca cacgacggag gagcggcctt ctcccac
//
LOCUS       DAOOTK010000154         6434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7443_length_6434_cov_5.614987, whole genome shotgun sequence.
ACCESSION   DAOOTK010000154 DAOOTK010000000
VERSION     DAOOTK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7443_length_6434_cov_5.614987"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PK634_12905"
     CDS             complement(<1..631)
                     /locus_tag="PK634_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98675.1"
                     /translation="MVLGLRAGAITIGAGPAIGTDKRGTTWYQEFQDWGQSDVRALDA
                     NDDEYKFYNAEDTGRDLVALYSHDDGTNVFFRMDFFELGYGWESGSVDVYLAIDCAPG
                     GQEWLPDYMDTKTDRPWEGCVGVYNSSAGSLYDAGWTNHASDYLGSYWRNDLDGVEFG
                     IKRSFLTARGWNGAATSLYLQAFSCRDGTDHGSGEIDVDASDIVDHFATL"
     gene            complement(952..2823)
                     /locus_tag="PK634_12910"
     CDS             complement(952..2823)
                     /locus_tag="PK634_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98676.1"
                     /translation="MKKFVALFLLVVLLLPAARAADLPPVMVTVEIRGIDALAEQVGL
                     LASDVGAPLTKEAVLQTLGGFMMAPDFQGLATDGTIRLFVMDPQSAGSQGEPPVLISL
                     PVKGDGSEYLEAVSTFMKPGEAVEGVTEYTADPASPSPLPLQSVFVAPVDGQLLVGAQ
                     QSLVRDGVQALGEGKLASANLPASGTIVFSVPCRRNADKLEPLIEQIAQSATATPTEA
                     PIDAARVVQAEFKALIKLLRQTENVMIGLKADGNALTLSTLVEPAAGTVLQRVIGELR
                     VPSAKVASLVPGNALMAASGFFVLPDEFLEGYAEMTDEMYGAMGEPFAGLAPVVREGL
                     EAIKGQFAGDYAFGVVPGSGNLPVDIIQVAAIRDTDQVKAAVEKMTAAMQKMMDAAST
                     QAAEGAEKPPFSMKFDIGEARSYEGVDITPYHYVIELPEEAKNEIPEFLSKWFTDLKY
                     ELAYVDNHIVYTLGSSKAMDGVIDALKGGGGVPVSADAACRALLPDLTASATDVSWIK
                     ILDIVRTFMRAVPDVPAELVDGLPATSLMLAGFSQAENGNLASHIRLSRSDLAAMAQY
                     FQSMAASRMQAAQGIEVEETMIEDEGTGETIEEIDVEAADEAAAGEATQEEVEVEEE"
     gene            2971..3804
                     /locus_tag="PK634_12915"
     CDS             2971..3804
                     /locus_tag="PK634_12915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98677.1"
                     /translation="MRLSVIIPTRDRPDRARRLIEALSAQPEAPEAEWILVDDGSAES
                     AARILADLASELAARGWNARRLRHETARGVSAARNAGARAAAGDILLFIDDDILPEAG
                     SLACCRRLHAQHPEVLVLNGRLRPLRRDYYARYWQHYYAAAFCRPGAGPLYTVDRISS
                     GFLSVKRRLLDLARPLFDETLTSREDFDLWLRLRDLGIPAYKADELAAGIETRTTLRR
                     FLDQRLWYARGERQLRRKHGAEKVAAEEARLVAPNRLAFLPLQLLSRLWLRLHGFGRD
                     A"
     gene            3801..4760
                     /locus_tag="PK634_12920"
     CDS             3801..4760
                     /locus_tag="PK634_12920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98678.1"
                     /translation="MTAPGTAAPFVSVVVLTRNRARLLAQCLDSLLRQDYPADRMELV
                     VVDDGSTDGTAEYLQTMAARDARVRRAGGEHRGIPAARNAGIRAARGEFIAIVADDYL
                     LAPDYVRTFVSFFRERPDASVVRFRIVPARDNLSSRLSHFAYEISLRKRLDPDPGPAP
                     RGLRERLRFYVRPPPLPPPAITADHRLEAAGAAAYRREVFERVGLFDESLGRAEDSDM
                     GRRLRDRGAAIYFHPFHTVAHQYGPWREEMLAKSFRAGIYHRRLAQRGVAGAEAGGAG
                     HVIRRAIQMKGLLAAWLYLPSVLAAELARRAGMGYGRMLDRKK"
     gene            complement(4784..>6434)
                     /locus_tag="PK634_12925"
     CDS             complement(4784..>6434)
                     /locus_tag="PK634_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98679.1"
                     /translation="YPDLMLVGHNHIFSTLASSPYYVLSSGPGWNYGNVGFFEFRKSG
                     DAWSCSNIAVHGGSNKLNAFGDWGAPTLVTNRFLAANDGAAWTNTAYITNALAQDFWD
                     GRLRFLLRKSAAGYSVSGGEKLAEYDYTASNSAVLVKVNIRHNALTTVTVSPTFADTD
                     PPALAFSVPTNGVVYRTSASSFWISGTVADAGGVTSVTWSASGATTASGGAATGLVAL
                     VAPGATWKYLDNGSDQGTAWRAAGFDDAAWASGPAEFGYGDGDEATTNSYGPDPNNKY
                     VTTYYRRSFEVGDPRVFTNALLVAAVRDDGILAFLNGAEALRNYMPAGDIAYTNFASS
                     TASGADETNWQAGTVSAGALTQGLNVVAAEVHQVNLTSSDISFNLALGADLGGSWLVT
                     NAVLASGTSIVSVVARDASGNASTGTLTVVVTDDVDDDELPDDWEIAHFGDTETSGGG
                     GDDEDEDGFTDWAEYVAGTDPMSETSFLAMEATASTATGWIIRWPGGTNRAYDLYQTT
                     NLFQPFTELATNLPAALPLTSYTVPVSAAQGVYLRIRVRNP"
BASE COUNT          954 a         2184 c         2248 g         1048 t
ORIGIN      
        1 ccagcgtcgc gaagtggtcc acgatgtcgc tggcgtcgac gtcgatctcg ccgcttccgt
       61 ggtctgtccc gtcccggcag ctgaacgcct gcaggtagag gctcgtggcc gcgccgttcc
      121 agccgcgcgc ggtcaggaac gagcgcttga tcccgaactc gacgccgtcg aggtcgttcc
      181 gccagtaact gccgaggtag tcgctcgcgt ggttggtcca cccggcgtcg tacagcgagc
      241 ccgccgacga attgtacacg cccacgcagc cctcccacgg ccggtcggtc ttcgtatcca
      301 tgtagtccgg cagccattcc tgcccgccgg gcgcgcagtc gatcgcgaga tacacgtcca
      361 ccgagccgct ctcccagccg tacccgagct cgaagaaatc catgcggaag aacacgttcg
      421 tgccgtcgtc gtgcgagtag agcgcgacga ggtcgcggcc ggtgtcctcg gcgttgtaga
      481 acttgtactc gtcgtcgttc gcgtccagcg cgcgcacgtc gctctgcccc cagtcctgga
      541 actcctggta ccaggtcgtg ccccgcttgt ccgtcccgat cgccggcccg gcgccgatcg
      601 tgatggcgcc cgcgcgcagg ccaaggacca tgccaagcac ccagcacagt aaaatcgttt
      661 tacttctcat acttttccct catttctgcc tgcggtgaat ggcgttactg tacccgtcat
      721 cggcgctttt gaaaagggca aaggacgcgc gcgcccggga agccgcgcaa cgccgcggga
      781 cggttttcca atgtttggaa aattcagcgc aaatttttcc aatgtttgga aaaaacgcgg
      841 gattattttc caaacattgg aaaagcccgc tgcgcggcct tgaatccggc cccgaaacac
      901 gacgggcggc ccggaggccg cccgtcgata ccgcgcaacc gaggacgcgc gttattcctc
      961 ttcgacttcc acttcttcct gcgtcgcctc gccggcggcg gcctcgtcgg cggcctcgac
     1021 gtcgatctcc tcgatcgtct cgccggttcc ctcgtcctcg atcatggtct cttcaacctc
     1081 gatgccctgc gcggcctgca tccggctggc ggccatgctc tggaaatact gggccatcgc
     1141 cgccaggtcg gagcgcgaca ggcggatgtg ggaggccagg ttgccgttct cagcctgcga
     1201 gaagccggcg agcatcagcg acgtggcggg cagaccgtcc acgagctcgg ccgggacatc
     1261 cggcacggcc cgcatgaagg tccggacgat gtccagtatc ttgatccagc tgacgtcggt
     1321 cgccgaggcc gtcaggtccg gcagcagggc gcggcaagcc gcgtccgcgc tcacggggac
     1381 accgcccccg cccttcagcg cgtcgatgac gccgtccatg gccttggagg agccgagggt
     1441 gtagacgata tggttgtcga cgtacgcgag ctcgtacttc aggtcggtga accacttgct
     1501 gaggaactcg gggatctcgt tcttggcctc ttccggcagc tcgatgacgt agtggtacgg
     1561 ggtgatgtcc acgccctcgt acgagcgggc ttcgccgatg tcgaacttca tgctgaaggg
     1621 cggcttctcg gcgccttccg cggcctgcgt ggaggcggcg tccatcatct tctgcatggc
     1681 ggccgtcatc ttctccacgg cggccttgac ctggtccgtg tcgcggatgg ccgcgacctg
     1741 gatgatatcc accggcaggt tgccggagcc cgggacgacg ccgaaggcgt agtcgccggc
     1801 gaactggccc ttgatggcct cgaggccctc gcggacgacc ggcgccaggc cggcgaacgg
     1861 ctcgcccatg gcgccgtaca tctcgtcggt catctcggcg tagccttcca ggaactcgtc
     1921 cggcaggacg aagaagccgc tcgcggccat cagggcgttg cccgggacca gcgacgccac
     1981 cttcgccgag ggcacgcgca gttcgccgat gacgcgctgc aggacggtgc cggcggcggg
     2041 ctcgaccagc gtcgagagcg tcagggcgtt gccgtcggcc ttgaggccga tcatgacgtt
     2101 ttccgtctgg cgcaggagct tgatcagggc cttgaactcg gcctggacga cgcgcgcggc
     2161 gtcgatcggc gcctcggtcg gcgtcgcggt cgcggactgg gcgatctgct cgatcagcgg
     2221 ctcgagcttg tcggcattgc ggcggcacgg cacggagaag acgatggtgc cggaggccgg
     2281 caggttggcg gacgccagct tgccctcgcc gagggcctgc acgccgtcgc gcacgagcga
     2341 ctgctgggcg ccaacgagca actggccgtc cacgggggcc acgaatacgc tctgcaacgg
     2401 cagcggcgac ggggaggcgg ggtcggcggt gtattccgtc acgccctcca cggcctcgcc
     2461 gggcttcatg aaggtcgaga cggcctcgag gtattccgag ccgtcgccct tgaccggcag
     2521 ggagatcagg accgggggtt cgccctggga accggcggac tggggatcca tgacgaacag
     2581 gcggatcgtg ccgtcggtgg ccaggccctg gaaatcgggc gccatcatga agccgccgag
     2641 ggtctggaga acggcttcct tggtgagtgg cgcgccgacg tccgaggcga gcaagccgac
     2701 ctgctcggcc agcgcgtcga tgccgcggat ttcaacggtc accatcacgg gggggagatc
     2761 cgccgcgcgc gcggcgggca gaagcaggac gaccaacagg aacaatgcta cgaacttctt
     2821 catacatgct cctttcatgc taggatgaac cgcacccgcc ccttatacgc ccggcggccg
     2881 gccggcgcca gtggattttt tccggccgcg caggcgccgg attgaccccg tttccagccc
     2941 cgcctacact gttcgaccag acgcgccgcc atgcgacttt ccgtcatcat accgacccgg
     3001 gaccggccgg accgggcccg ccggctgatc gaggccctgt ccgcccagcc ggaggctccg
     3061 gaggcggaat ggatccttgt ggacgacggc agcgcggaat ccgccgcgcg gatcctggcc
     3121 gacctggcgt ccgagctcgc cgcccggggc tggaacgcgc ggcggctccg ccacgagacg
     3181 gcgcgcggcg tatccgcggc gcgcaacgcg ggggcgcgag ccgccgccgg ggacatcctc
     3241 ctgttcatcg acgacgacat cctgccggag gccggcagcc tcgcctgctg ccggcgcctt
     3301 cacgcgcagc acccggaagt gctcgtgttg aacgggcgcc ttcgccccct ccgccgcgat
     3361 tactacgccc gctactggca gcattattac gcggccgcct tctgccggcc gggcgccggt
     3421 ccgctgtaca ccgtggaccg gatctcgtcg ggattcctgt ccgtcaagcg ccgcctgctg
     3481 gacctcgcgc ggccgctgtt cgacgagacc ctgacctccc gcgaggactt cgacctgtgg
     3541 ctgcgcctgc gcgacctcgg cataccggcc tacaaggcgg acgagctggc cgccgggatc
     3601 gaaacacgga ccacgctgcg ccggttcctc gaccagcggc tctggtatgc gcggggcgaa
     3661 aggcaactgc ggcgcaagca cggcgcggaa aaggtcgccg cggaggaggc gcgcctggtc
     3721 gcgccgaatc ggctggcctt cctgccgctg caactgctgt cgaggctctg gctgcggctg
     3781 cacgggttcg ggcgcgacgc atgaccgcgc ccggaacagc cgcccccttc gtttcggtcg
     3841 tcgtcctgac gcgcaaccgg gcccgcctgc tcgcgcagtg cctggacagc ctgctgcgcc
     3901 aggactaccc ggcggaccgg atggagctcg tcgtcgtgga cgacggctcg accgacggca
     3961 cggcggagta tctccagacc atggccgcgc gtgacgcgcg cgtccgccga gcgggcgggg
     4021 agcaccgggg catcccggcg gcgcgcaacg cgggcatccg ggccgcgcgc ggcgagttca
     4081 tcgccattgt ggcagacgac tacctgctgg cgccggacta cgtgcgcacg tttgtttcgt
     4141 ttttccggga gcggcccgac gcctccgtgg tgcgcttccg gatcgtgccg gcccgggaca
     4201 acctgtccag ccggttgagc cacttcgcct acgagatcag cctgcgcaag cggctggacc
     4261 cggatcccgg gcccgcgccg cgcgggctcc gggagaggct ccgcttctac gtgcgccccc
     4321 cgcccctgcc ccccccagct atcacggccg accaccggct cgaggccgcg ggcgcggccg
     4381 cctaccgccg ggaggtattc gagcgcgtgg gcctgttcga cgaaagtctc ggccgcgccg
     4441 aggacagcga catgggccgc cgcctgcggg atcgcggcgc agcaatatac ttccatccct
     4501 ttcataccgt ggcccaccag tacggcccgt ggagagaaga gatgctggcc aagagtttcc
     4561 gcgcgggcat ctatcaccgg cgcctcgcgc agcgcggcgt ggccggcgcg gaggcgggcg
     4621 gagcgggaca cgtgatccgg cgggcgatcc agatgaaggg cctgctcgcc gcgtggctgt
     4681 acctgccctc ggtgctggcg gccgagctcg cgcgtcgcgc ggggatgggg tatggccgga
     4741 tgctggatcg aaaaaaatga gggcggcggg aggcttccgt gcttcaggga ttccgcaccc
     4801 ggatccgcag gtagacgcct tgcgcggcgc tgaccggcac ggtgtagctc gtcaggggca
     4861 gcgccgccgg caggttggtc gccagttcgg tgaagggctg gaacaggttg gtcgtctggt
     4921 agaggtcata ggcgcggttc gtcccgccgg gccagcggat gatccatccc gtcgccgtcg
     4981 acgcggtcgc ctccatcgcc aggaatgagg tctcgctcat cggatcggtg cccgccacgt
     5041 actcggccca gtccgtgaac ccgtcctcgt cctcgtcgtc gccgccgccc ccggaggttt
     5101 ccgtgtcgcc gaagtgcgcg atctcccaat cgtccggcag ctcgtcgtcg tccacgtcgt
     5161 ccgtgacgac gacggtcagc gtgccagtgc tcgcgttgcc ggaggcgtcg cgcgcgacca
     5221 cggaaacaat gctggtgccg gaggcgagca cggcgttcgt gacgagccag gagccgccga
     5281 ggtccgcgcc gagggcgagg ttgaagctga tgtcgctgct ggtgaggttg acctggtgga
     5341 cctccgccgc gacgacgttc aggccctggg tcagtgcgcc cgcgctcacc gtcccggcct
     5401 gccagttggt ctcgtccgcg ccggacgccg tggagctggc aaagttggtg tacgcgatgt
     5461 cgcccgcggg catgtaattc cggagcgcct ccgcgccgtt gaggaaggcg aggatgccgt
     5521 cgtcccgcac ggcggcgacg aggagcgcgt tggtaaacac gcgcggatcg ccgacctcga
     5581 aggagcggcg gtagtaggtg gtcacgtact tgttgttcgg gtcggggccg tagctgttcg
     5641 tggtcgcctc gtcgccgtcg ccgtagccga actcggcggg gccggaggcc caggccgcgt
     5701 cgtcgaagcc cgccgcgcgc caggccgtgc cctggtcgct gccgttgtcg aggtacttcc
     5761 acgtcgcgcc gggggcgacc agcgcgacga ggccggtcgc ggcgccgccg gaggccgtcg
     5821 tggcgcccga agcggaccac gtgacggacg tgacgccgcc ggcatccgcg accgtgccgg
     5881 agatccagaa actggaggcc gaggtccggt acacgacccc gttggtgggc accgagaagg
     5941 ccagcgccgg agggtcggta tcggcgaagg ttggggagac ggtgaccgtg gtcagcgcgt
     6001 tgtgccggat gttgaccttt accagcacgg cgctgttgct ggccgtgtag tcgtactcgg
     6061 cgagtttttc gccgccgctg acggagtagc cggcggcgga cttgcgcagg aggaaacgca
     6121 ggcgcccatc ccagaaatcc tgggccagcg cgttggtgat gtaggccgtg ttcgtccagg
     6181 cggcgccgtc gttggcggcc aggaaccggt tggtgacgag cgtcggcgcg ccccagtcgc
     6241 cgaaggcatt gagcttgttc gaccccccgt ggaccgcgat gttggaacag gaccacgcgt
     6301 cgccggactt gcggaactcg aagaacccga cgttgccgta gttccagccc ggaccggagg
     6361 agaggacata gtacgggctg gaggccagcg tcgagaagat gtggttgtgg ccgacgagca
     6421 tgagatcggg atac
//
LOCUS       DAOOTK010000155         6417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7475_length_6417_cov_4.767526, whole genome shotgun sequence.
ACCESSION   DAOOTK010000155 DAOOTK010000000
VERSION     DAOOTK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7475_length_6417_cov_4.767526"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PK634_12930"
     CDS             <1..675
                     /locus_tag="PK634_12930"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOW98680.1"
                     /translation="RYYLTCEMLGGDLFDVFTLDNDHVGMYMADVAGHGVSAALISGL
                     LKMAVTSARESPGGEAGALTARLIQPDKTLSTINDMLVKELPEYEFITMIYAVLDIPT
                     CTFHVASAGHLSPLKYEAATRQVVSWDIPTETALGLVEKCAYTTVQRTVAPGDKILFF
                     TDGLVEAMNVRGEEFGEEALRRQFGQQALQPPAQIITALRQSVEQHRGGEKVSDDYSI
                     LVAEIR"
     gene            complement(683..1990)
                     /locus_tag="PK634_12935"
     CDS             complement(683..1990)
                     /locus_tag="PK634_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOW98681.1"
                     /translation="MNRAKVAVLRTKPGTVLEDIHRLMNLAGYRDVLPRDRETALKIN
                     ISWHFFYPASSTTPWQLDGVIRALKRDGYDPARLHGCHNRTVVIDAHLGERENKQLPV
                     IEAHGLKNVHLYEGEEWVHVRDAVGDLADRFLCLNKVYPDGFMIPKRFIGENIIHLPT
                     VKTHVFTTTTGAMKNAFGGLLNERRHWTHPVIHETLVDLLMIQKKIHSGVFAVMDGTF
                     AGDGPGPRCMVPHVKNVLLASADQVAIDAVAAKLMGFDPLADLRFVRLAHELGLGCGD
                     PREIEIVGDADAAAENWHFNGPFRKMTFASRMQHLIYWGPLKKPIEWSLKTVLAPWAY
                     IASVMYHDSFWYPTHRKIVNDILGSEWGRLFQNWERLRIPPDDLAAPGWADVGAAPAE
                     LKAESLKYFKQSLKILGTCIREAPEFAARKRRKAARAESPTRT"
     gene            2121..2486
                     /locus_tag="PK634_12940"
     CDS             2121..2486
                     /locus_tag="PK634_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOW98682.1"
                     /translation="MPAPPLNIQTARSGDAVVIKLDGIVDASTIDALRRTLEPLCAQS
                     AARIVVDGSLLTYINSLGFGLLFKLSQSCRALQGRLVVAGLQDKLRVVFKILGLEKLI
                     QFAPTCTEALASLAANSKP"
     gene            2483..2932
                     /locus_tag="PK634_12945"
     CDS             2483..2932
                     /locus_tag="PK634_12945"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOW98683.1"
                     /translation="MNKTMASSAENVQIRVPGEARFLALIRSVTTMLARNAGFPDVDV
                     DKIELAVDEACSNVVEHAYRKASPKPAIELEIRWEPGRFIVDIVDRGIPFDFAKHVTP
                     KFPDHWISGECRGAGLFLIRSCMDETEYESQPGQANRLRLVKRLQPT"
     gene            complement(2946..4325)
                     /gene="thrC"
                     /locus_tag="PK634_12950"
     CDS             complement(2946..4325)
                     /gene="thrC"
                     /locus_tag="PK634_12950"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOW98684.1"
                     /translation="MNYISTRGRTPPMGFQDAVLTGLAPDGGLLIPTELPRVDDRLEA
                     WSKLSYQDITFEILRLFVDLPDADLRHLIERSYMTFRHPEIAPAWPVGPVHILELFHG
                     PTLAFKDIALQFLSNFFEYILKRRDGHLNILGATSGDTGSAAIHGVRGRERINIFIMH
                     PHGRVAPLQEKQMTSVLDSNVFNIAVEGTFDDCQRIMKTLFNDLAFKQRHALGAVNSV
                     NWARVLVQIVYYFSSGLYVMQTTGAKEVQFAVPTGNFGDVLAGYYAWRMGLPIRRLIL
                     ATNENDILARFFETGVYSLGKVVPTLSPSMDIQVASNFERYLYCKVGGDPARLTGLMN
                     DFAARGALSIPVGPSGRVDELFIPGVGTTADTLATIRRYDREHGYLLDPHTAVGVRVA
                     EQHLAPGAPTICLATAHPAKFSQAIREATGREARHEILDNLAHAPTRCEVLPADTGAI
                     KAFIEQRVG"
     gene            complement(4424..5218)
                     /locus_tag="PK634_12955"
     CDS             complement(4424..5218)
                     /locus_tag="PK634_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOW98685.1"
                     /translation="MATGLVALAALAGVDEDLLLDATRYGSTPSKRAVKEKARAELFS
                     RGPDGLRLVMRHVHLENVGVQGFAFEMVQELGARAAPVLVEFLGDEQALTRQLAAWYL
                     GFGDTPEYAAPVLELLSDEKACGAAMRTLGKWRVRAAVPAILPFTRHERETRRVVAIN
                     ALRDIGDPAAIPALRDALNDPFFTVREAAARALASLGRPAEQALLRALPGAKGGVRRH
                     MVRALGAMRSRRAAGALRKLFKAEDPFVREDAAEALDRIRGARPVP"
     gene            complement(5258..>6417)
                     /locus_tag="PK634_12960"
     CDS             complement(5258..>6417)
                     /locus_tag="PK634_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98686.1"
                     /translation="LLRVNTELGPVFVGTPGRDEYSGRALLILDPDGDDIYRGAAGVA
                     DGRTGQGLAAIVDLKGDDSYKADGLWGAGSALWGAAALLDLEGNDLHEARYAGQGAAL
                     CGAAWLEDRGGDDVYRAGGFAQAAAFYGVAVLRERAGRDLYDVGLAGQAYAGVLGFAL
                     LADGAGSDRYLAGGREPDHERNDDRHLSLAQGFSIGMRPWAGGGVAALVDLEGNDAYI
                     ADIYGQGVSYWYGAGFLLDGGGNDTYQLYQYGQGAGIHLSLGLLADRGGSDQYTGYIL
                     CQGAAHDYGVGMLFDQGGDDTYSADHHSQGRALNNALALLVDSGGDDAYFGRQPDQCQ
                     GVGNDGGHREYGSLALLLDLAGADRYSCGAADGARMKRPDFGIVYDVQSDP"
BASE COUNT         1015 a         2280 c         2099 g         1023 t
ORIGIN      
        1 cgctactacc tcacctgcga aatgctgggc ggcgacctgt tcgacgtctt caccctggac
       61 aacgaccacg tcggcatgta catggcggac gtcgccgggc acggcgtcag cgccgccctc
      121 atctccggcc tgctcaagat ggcggtcacg tcggcgcgcg aaagccccgg cggcgaggcc
      181 ggcgcgctga ccgcccggct gatccagccg gacaagacgc tctccaccat caacgacatg
      241 ctggtgaagg agctgcccga gtacgagttc atcaccatga tctatgccgt gctcgacatc
      301 ccgacctgca ccttccacgt cgcgagcgcc ggccacctct cccccctgaa gtacgaggcc
      361 gcgacccgcc aggtggtctc gtgggacatc ccgaccgaga ccgcgctggg gctcgtcgag
      421 aaatgcgcct acaccaccgt ccagcggacc gtggcgccgg gagacaagat cctgttcttc
      481 accgacgggc tcgtcgaggc catgaacgtg cgcggcgagg aattcggcga ggaggccctc
      541 cgccggcagt tcgggcagca ggccctccag ccccccgcgc agatcatcac cgccctgcgg
      601 cagtcggtgg aacagcaccg cggcggcgaa aaggtcagcg acgactactc catcctcgtc
      661 gcggaaatcc gctgacggcc gctcacgtcc gggtgggcga ttccgccctc gccgccttcc
      721 gccgcttgcg cgccgcgaac tcgggcgcct cccggatgca ggtgccgagg atcttcaggg
      781 actgcttgaa atacttgagg ctctcggcct tcagttccgc cggcgccgcg ccgacgtccg
      841 cccagcccgg ggcggccagg tcatccggcg gaatccgcag gcgctcccag ttctggaaga
      901 gccgccccca ctcgctgccg aggatgtcgt tcacgatttt ccggtgggtc gggtaccaga
      961 acgagtcgtg gtacatcacg ctggcgatgt aggcccacgg cgccaggacg gtcttcagcg
     1021 accactcgat gggtttcttc agcgggcccc agtagatcag gtgctgcatg cggctcgcga
     1081 aggtcatctt ccggaacggg ccgttgaaat gccagttctc cgcggcggcg tccgcgtctc
     1141 cgacgatctc gatctcccgc gggtcgccgc acccgagccc gagctcgtgg gccaggcgca
     1201 cgaaccggag gtccgccagc gggtcgaagc ccatcagctt cgccgccacc gcgtcgatgg
     1261 ccacctggtc ggcggaggcg agcaggacat tcttgacgtg cggcaccatg cagcgcgggc
     1321 ccggcccgtc gccggcaaag gtgccgtcca tgacggcgaa gacgccgctg tggatcttct
     1381 tctggatcat cagaaggtcc acgagcgtct cgtggatcac cgggtgcgtc cagtggcgcc
     1441 gctcgttcag cagcccgccg aaggcgttct tcatcgcgcc cgtcgtcgtg gtgaacacgt
     1501 gcgtcttgac cgtcggcagg tggatgatgt tctcgccgat aaagcgcttc gggatcatga
     1561 agccgtcggg gtagaccttg ttcaggcaca ggaaccggtc cgccaggtcg cccacggcgt
     1621 cccgcacgtg gacccactcc tcgccctcgt agaggtgcac gttcttcagc ccgtgggcct
     1681 cgatcacggg aagctgcttg ttctcccgct cgcccaggtg ggcgtcgatg acgaccgtcc
     1741 ggttgtggca gccgtggagg cgggcggggt cgtagccgtc ccgcttgagg gcgcggatga
     1801 ccccgtcgag ctgccagggg gtcgtggaac tggccgggta gaaaaagtgc cagctgatat
     1861 tgattttcag ggcggtttcg cggtcccggg gcaggacatc ccggtacccg gccaggttca
     1921 tcaggcgatg gatatcctcc agcaccgtcc ccggcttcgt ccgcagcacc gccaccttgg
     1981 ctcgattcat tgtttcatcc gcctttcaga attatatacc ggccaccggc ccgccgcaag
     2041 gaccggcttt ccttgacatg cggaggccta tatttcaaca tccagtcggg gtatatatta
     2101 acaaaatgga ttagaccaat atgccggccc cgccgctcaa catacaaacg gcccgttcgg
     2161 gagatgccgt ggtgatcaag ctggacggca tcgtggacgc gtcgaccatt gacgcgctgc
     2221 ggcgtaccct cgagccgctg tgcgcgcagt ccgcggcccg catcgtggtg gacgggtcgc
     2281 tgctgaccta catcaacagc ctggggttcg ggctcctctt caagctgtcc cagtcctgcc
     2341 gggcgctgca gggccgcctg gtcgtggccg gcctgcagga caagcttcgc gtcgtgttca
     2401 agatcctggg gttggagaag ctgatccagt ttgcgccgac ctgcacggag gccctggcct
     2461 ccctcgccgc aaacagcaaa ccgtgaacaa gacgatggcc agcagcgcgg aaaacgttca
     2521 gattcgcgtc cccggcgagg cgcgcttcct cgcgctcatc cgctccgtca ccaccatgct
     2581 ggcccgcaac gccggattcc ccgacgtgga cgtggacaag atcgagctcg ccgtggacga
     2641 ggcctgctcg aacgtcgtgg agcacgccta ccgcaaggcc tctcccaagc ccgccatcga
     2701 actggaaatc cggtgggagc cgggccggtt catcgtggac atcgtggacc ggggcatccc
     2761 gtttgatttc gcgaagcatg tcacgccgaa gttcccggac cactggatca gcggggaatg
     2821 ccgcggcgcg ggcctgttcc tgatccggtc ctgcatggac gagaccgagt acgagagcca
     2881 gcccggccag gccaaccgcc tccggctggt caagcggctc cagcccacgt gaccgcccgc
     2941 gcggttcagc cgacccgctg ctcgatgaag gccttgatcg cgcccgtgtc cgcgggcagc
     3001 acctcgcacc gcgtcggcgc gtgcgccagg ttgtcgagga tctcgtggcg cgcctcgcgt
     3061 cccgtcgcct cgcggatggc ctggctgaac ttcgccgggt gcgcggtggc caggcagatc
     3121 gtcggcgcgc cgggcgcgag atgctgctcc gccacgcgca cgccgaccgc cgtgtgcggg
     3181 tcgagcaggt agccgtgctc ccggtcatac cgccggatcg tcgccagcgt gtcggccgtc
     3241 gtgccgacgc cggggatgaa cagctcgtcc acgcggccgc tgggcccgac cggaatcgac
     3301 agggcgccgc gcgccgcgaa gtcgttcatc agccccgtca gccgggccgg gtcccctccg
     3361 accttgcagt agaggtagcg ctcgaagttg ctggccacct ggatgtccat cgaggggctc
     3421 aacgtcggga cgaccttccc gaggctgtag acgccggtct cgaagaaccg cgcgaggatg
     3481 tcgttctcgt tggtcgccag gatgagccgg cggatgggca ggcccatgcg ccacgcgtag
     3541 tagccggcca ggacgtcgcc gaagttgccc gtcggcacgg cgaactggac ctccttcgcg
     3601 ccggtggtct gcatgacgta gaggccggag ctgaagtaat agacgatctg gaccagcacc
     3661 cgcgcccagt tcaccgagtt caccgcgccg agcgcgtgcc gctgcttgaa cgcgaggtcg
     3721 ttgaacagcg tcttcatgat ccgctggcaa tcgtcgaagg tcccctcgac ggcgatgttg
     3781 aagacgttcg agtcgagcac ggacgtcatt tgcttctcct gcagcggcgc gacgcggccg
     3841 tgcgggtgca tgatgaagat attgatccgc tcgcggccgc gcacgccgtg gatggcggcg
     3901 ctgcccgtgt cgccgctggt cgcgccgagg atgttcaggt gcccgtcgcg ccgcttgagg
     3961 atgtactcga agaaattgct caggaactgc agcgcgatgt ccttgaacgc cagcgtgggg
     4021 ccgtggaaca gttccaggat gtgcacgggc cccaccggcc acgccggcgc gatctcggga
     4081 tgccggaagg tcatgtagct gcgctcgatc aggtggcgca ggtccgcgtc cggcaggtcg
     4141 acgaagaggc gcaggatttc aaacgtgatg tcctggtagg aaagcttcga ccacgcctcc
     4201 agccggtcgt ccacccgcgg cagttcggtc gggatgagca ggccgccgtc cggcgccagc
     4261 ccggtgagca cggcatcctg gaatcccatg ggcggggtcc ggccccgcgt gctgatgtag
     4321 ttcatgatgt cctcgggcgc gcaggatacg cgggcccgga ccgattcgac aagcccgatt
     4381 cgcccggcgg ggaaatttcc acactttgca aagtgtggaa atttcagggg acgggccgcg
     4441 cgccccggat tcgatccagg gcctcggcgg catcctcgcg gacgaaggga tcctcggctt
     4501 tgaacagctt ccgcaaggcc cccgccgcgc ggcgcgagcg catcgcgccc agcgcgcgca
     4561 ccatgtggcg gcggacgccc ccttttgccc ccggcagcgc ccgcagcagc gcctgttccg
     4621 ccggccgccc cagcgaggcc agcgcccgcg cggcggcctc ccgcaccgtg aaaaagggat
     4681 cgttcaacgc atcccgcagg gccgggatcg ccgccggatc gccgatgtcg cgcaaggcat
     4741 tgatggcgac cacgcgccgg gtctcccgct cgtgcctcgt gaacggcagg atggccggca
     4801 cggcggcgcg gacccgccac ttgcccagcg tccgcatcgc ggctccgcag gctttctcgt
     4861 cggacaacag ctccagcacg ggcgccgcat attccggcgt gtcgccaaag cccaggtacc
     4921 acgccgccaa ctggcgggtc agcgcctgct cgtcgcccag gaattccacc agcacgggcg
     4981 ccgccctggc gcccagttcc tgcaccattt cgaaagcgaa cccctggaca ccgacatttt
     5041 ccaggtggac atgccgcatc accaggcgca ggccgtcggg gccgcgcgag aacagctcgg
     5101 cccgggcctt ttccttcacc gcccgcttcg acggggtcga gccgtagcgg gtcgcgtcga
     5161 gcaggaggtc ctcgtccacg ccggccagcg cggccagcgc gacgaggcct gtcgccatcg
     5221 ccgccgctat gagccttcta gaagacatcg gcgtccatca tgggtccgat tgcacgtcgt
     5281 acacgatccc gaaatccgga cgcttcatcc gcgcgccgtc cgcggcgccg cacgagtagc
     5341 ggtcggctcc cgcgaggtcc agaagcagcg ccaggctgcc gtactcgcgg tgacccccgt
     5401 cgttgccgac cccctggcac tggtcgggct gccggccgaa gtaggcatcg tcgccgccgg
     5461 aatccacgag aagggccagg gcattgttca gcgcgcggcc ctgggagtgg tggtccgccg
     5521 aataggtatc gtcgccgccc tggtcgaaca gcatgcctac gccgtaatcg tgcgcggcgc
     5581 cctggcagag gatatagccg gtgtactggt ccgatccccc gcggtccgcc agcaagccga
     5641 ggctcaagtg gatgcccgcg ccctgcccat actggtacag ctgataggtg tcgtttccgc
     5701 cgccgtccag caggaacccg gcgccgtacc agtagctcac gccctgcccg tagatatccg
     5761 cgatgtaggc gtcgttccct tccaggtcca cgagcgccgc cactccgccg ccggcccagg
     5821 gccgcatgcc gatggagaac ccctgcgcga gcgagaggtg ccggtcgtcg ttacgctcat
     5881 ggtccggctc acggccgccg gccaggtacc gatcgctccc cgccccgtcc gccagcagcg
     5941 cgaaacccag cacgccggca taggcctggc ccgccagccc gacgtcgtac aggtcgcgtc
     6001 cggcgcgctc gcgcagcacg gccacgccgt agaaagccgc ggcctgcgcg aatccgccgg
     6061 cccggtagac gtcatccccg ccccggtcct cgagccaggc ggctccgcac agcgcggcgc
     6121 cctgcccggc gtaacgggcc tcgtgcaggt cgttgccctc caggtccagc agggccgccg
     6181 cgccccacaa ggccgagccg gcgccccaca agccgtccgc cttgtacgag tcatcccctt
     6241 tcaggtccac gatcgcggcc agcccctgcc cggtccggcc atccgccacg ccggcggcgc
     6301 cccggtagat gtcgtcgccg tccggatcga ggatcagcag ggcccgcccg gaatactcgt
     6361 cgcgtcccgg cgtgccgacg aacaccgggc ccagctcggt gttcacacgc aaaagcg
//
LOCUS       DAOOTK010000156         6348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7588_length_6348_cov_6.925949, whole genome shotgun sequence.
ACCESSION   DAOOTK010000156 DAOOTK010000000
VERSION     DAOOTK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_6348_cov_6.925949"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..430)
                     /locus_tag="PK634_12965"
     CDS             complement(38..430)
                     /locus_tag="PK634_12965"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOW98687.1"
                     /translation="MKTGYEFLRAAAVVVVACSLSANLARAHCDTMKGPVIPEAKAAL
                     GKGDVTPVLKWIKEGDEAEIKEAFAKALLVRAKGPEAQELADKSFLETLVRLHRAGEG
                     APYAGLKDEAVAPIVAMADKAVASPLIA"
     gene            complement(711..3482)
                     /locus_tag="PK634_12970"
     CDS             complement(711..3482)
                     /locus_tag="PK634_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOW98688.1"
                     /translation="MNILRRWGFLPVALLGFFGFPEHTRAAYSLILTNGYGDCYRMNM
                     LRDRYNYQAYRGVCSYRGFSLSGCSTTGEVVMLRIKATSSMPEHFSIVSTSLRWGTNM
                     YFLNDVFRWLNSSVMAGYENWYPFDMSCTVAMYVYSGGFSVAGGVPYAADEGPLDAGR
                     AGPPMDEQRLMMELLNEPAAAPAEPHAYSLVMTNGYGDCYRMNMLRDRYNYQAYRGVC
                     SYRGFSLSGCSTTGEVVMLRIKATSSMPEHFSIVSTSLRWGTNMYFLNDVFRWLNSSV
                     MAGYENWYPFDMSCTVAMYVSSGGFGAHGTERAAVRAWPGRPASRVSTASKADRPLRI
                     GALVPLTGDLEDIGSSYTSAYGKAISEITNALGMADVELLVENTDADPLVAAMKLEAL
                     YTNGVQVVVGPETSAECEALRLLATNGVGMLLVSSSATAVPLAIPNDPLMRLTMDDSH
                     QARALASRIADDGITNLAVLKRSDMYGGGMKDAFYAEYTNRGGTVFFDEYFPRSPDLF
                     GEVMSNVNEVVAARIAETGEDGVGLLIVAFDEGVRLLEESAGFPALTSVRWYGTEGMA
                     GNAALLTNVVSREVARRTRFICSQPAAYTNAKYAEVAAHIQGETGHVPRTFSVQSYDA
                     LWLAALALRDTGGTGSVEQVTQAIRTNAAVYSGATGPIVFNNADDRADGEYTFLKVSD
                     TQSWDNLLGAVPNAPIERSAVNVTSNRFDARWHLSVGATNHLLYVAEDPEGAACLPGF
                     DGASFGGAGSAAIEGLAPGQTYYYRVRAANAFGTSADSGTVTVRVWEAACATNGVPLW
                     WLGQYYPAEDVATNADGLALSDSDGDGHLAWEECVAGTSPVDSRSRFAVGGLALGSVS
                     NMVFQWESVTGRSYSLFRETNVVPGLWNESLFDSVPGTGETLYRTNPVDLGVPRAFFR
                     LVTEKTPSP"
     gene            complement(3934..4008)
                     /locus_tag="PK634_12975"
     tRNA            complement(3934..4008)
                     /locus_tag="PK634_12975"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3974..3976),aa:Thr,seq:tgt)
     gene            complement(4067..5131)
                     /locus_tag="PK634_12980"
     CDS             complement(4067..5131)
                     /locus_tag="PK634_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98689.1"
                     /translation="MRKYLSIMLALVGAGWAPPAPAQDETKPVEAVELVRSPQKYWAR
                     SFVFADTLRALPSGGLVKLDNRRYASFSTAVAGQCYVAAELVNLFNGLPLDRRYQFLG
                     TVIQYGRRFYIIVRGVSASMEASELKLDWPDADGAASDYMKNQALRPIVDILTLAESA
                     HVAYAEEKGVALEELYDPQSPHYGQALGLLRTTIMAHEQKVKMTASEMLAEYLFQMLA
                     ARHPAAARPAQPVEPPAAPETPAAPPAVEAPAPEPASTSVDTPREEPPKPLTRAERRE
                     LARQKKAEAAAARAEQKRQAAEAKKAAAAPAPKMPPTSAPEAETPAILDPTVEAARLE
                     IPAPPIEAPAAEALPETTTP"
     gene            complement(5236..5883)
                     /gene="nth"
                     /locus_tag="PK634_12985"
     CDS             complement(5236..5883)
                     /gene="nth"
                     /locus_tag="PK634_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOW98690.1"
                     /translation="MIKATPQLKRRAAEVLRRLRAEYPDADCELDFSNPLELVMAAIL
                     SAQCTDKRVNLVTPALFKKYRTAADWARTPQATLEKEIRSTGFYRNKAKNIRALGAAL
                     AKQFGGRLPEDLDTLVTLPGIGRKTANLLLVSAFRRPGIIVDTHFKRLSARLGFTDNE
                     DPDKIEFDLKAIVPEKDWGAWSHAMVFHGRRCCYARKPECPRCPVRGLCPSAGKV"
     gene            complement(6012..>6348)
                     /locus_tag="PK634_12990"
     CDS             complement(6012..>6348)
                     /locus_tag="PK634_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOW98691.1"
                     /translation="DDDGSVHIFSADKEAMEAAVREVSMVTAEAEPGKIYQGTVTGVK
                     EFGAFVEILPGQEGLVHISELANFRVKRTEDVVKVGDLVWVKCLSVDESGKIRLSRKA
                     ALEEKDAKS"
BASE COUNT         1134 a         2116 c         1948 g         1150 t
ORIGIN      
        1 gtgcccagaa gcgcttattg cgtgagcgtc tctacgctca tgcaataagc gggctggcca
       61 ccgctttgtc ggccatggcc acaatcggcg cgacggcttc gtccttcagc ccggcgtaag
      121 gggctccttc gcccgcgcga tgcaggcgaa cgagagtttc caggaaagac ttgtcagcca
      181 gttcctgcgc ctcaggtcct ttggcgcgaa ccaggagcgc cttcgcaaag gcctccttga
      241 tctcggcttc gtcgccttct tttatccact tgagcacggg agtaacgtcg ccttttccca
      301 aagcggcctt ggcttccggg ataacagggc ctttcatcgt gtcgcagtgc gctcgcgcaa
      361 gatttgcgga cagcgagcag gccacaacaa cgacagcggc agctctcaag aactcatatc
      421 ccgttttcat ttcggctccc ttcttgccaa tacacagacg gcaggcatgc atgggcccac
      481 ctaaggcggc aggctagcga acaggctgat cacggccagg cagaatacac ttcctgaaga
      541 ataacaacgg cgcgtgcgcg gccgattggc gaggcgccct accgagccga cggcgagagg
      601 cccagcgagc cgggaatact tgagcggaca cagtacaggc tgcaacatga tgatcagacc
      661 ggcggcaatt caaagtgaat tgttacggcc tgaccccacc cctcctgttc tcaagggctg
      721 ggcgttttct ccgtgaccag ccggaaaaag gcgcggggga cgcccaggtc caccggattc
      781 gtgcggtaca gggtttcgcc cgtccccgga acagagtcga acagggattc gttccagagg
      841 ccgggcacca cgttggtttc ccggaacagg gagtaggagc ggcccgtcac gctctcccac
      901 tgaaagacca tgttgctgac gcttcccagc gccaggcctc ccacggcgaa acggcttcgg
      961 gaatcaacgg gtgacgtccc cgcgacgcac tcctcccagg ccagatgccc atccccgtcg
     1021 ctgtcgctca aggccagccc gtcggcgttg gtcgcgacgt cttcggccgg atagtactgt
     1081 cccagccacc acaacggcac gccgtttgtc gcgcaggcgg cttcccatac gcggaccgtc
     1141 acggttccgg aatcggcgct ggttccgaag gcattggccg cgcgcacgcg atagtaatag
     1201 gtctgccccg gcgccaggcc ttcgatcgcc gcggaacccg cgcccccgaa agacgcgccg
     1261 tcgaagcccg ggagacaggc tgcgccctcc ggatcttcgg ccacatacag caagtggttc
     1321 gtggcgccga cggatagatg ccaccgcgca tcgaatcggt tgctggtgac gttcaccgcc
     1381 gaccgctcga tgggcgcgtt ggggaccgcc cctaaaaggt tatcccagct ctgggtatcc
     1441 gacaccttga gaaacgtgta ctcgccgtcc gcccggtcgt cggcgttatt gaagacgatc
     1501 ggtccggtgg ctcccgagta gaccgccgcg ttcgtgcgga tcgcctgcgt tacctgctcc
     1561 acggagcccg tcccgcctgt atcccgcagg gccagggccg ccagccagag cgcgtcatag
     1621 gattgcaccg agaacgtccg gggcacatgg ccggtctcgc cctggatatg ggcggccacc
     1681 tcggcatatt tcgcgttcgt gtacgccgcc ggttgcgaac agatgaaacg ggtccgacgg
     1741 gcgacctctc gagagacgac attcgtcaac agggcggcgt tacccgccat gccttccgtc
     1801 ccgtaccagc gcaccgaggt cagggcggga aaccccgcgg actcctccag cagtcgaacc
     1861 ccttcatcga acgccacgat cagcaggccg acgccgtcct cgccggtctc ggcgatccgc
     1921 gccgccacga cctcgttcac gttcgacatc acttcgccga acaggtccgg ggaccgcgga
     1981 aaatactcgt cgaaaaatac cgtgccgccc cggttggtgt attccgcata gaaggcgtcc
     2041 ttcatgccgc cgccgtacat gtccgaccgc ttcagcaccg ccaggttggt gatgccgtcg
     2101 tccgcgatcc ggctcgccag cgcccgcgcc tggtgcgaat cgtccatcgt cagccgcatc
     2161 agggggtcgt tcggaatcgc cagcggcacg gcggtggcgg agctggacac caacagcatc
     2221 ccgaccccgt tggtggctag gagccgcaac gcttcgcatt ccgcgctggt ttccggcccg
     2281 accaccacct gcaccccgtt cgtgtacagg gcctccagtt tcatggcggc caccaacgga
     2341 tcggcatcgg tattctccac cagcagttcc acgtccgcca tccccagcgc gttggtgatt
     2401 tccgagatcg cctttccgta cgcggaggtg tacgaggacc cgatgtcctc caggtcaccc
     2461 gttagcggca ccagcgcgcc gatgcgcagc ggtctatcgg ccttgctcgc cgtggagacc
     2521 cgcgaggccg ggcgccccgg ccaggcgcgg actgcggccc tctccgttcc atgggcgccg
     2581 aagccgccgc tgctgacgta catcgccacg gtgcaactca tgtcgaacgg ataccagttc
     2641 tcgtagcccg ccatgacaga agagttgagc caccggaaga cgtcgttcag gaaatacatg
     2701 ttggtgcccc accgcaggga cgtggaaaca atggagaaat gctcgggcat ggaggacgtc
     2761 gccttgatgc gcagcatcac cacctcgccc gtcgtggagc agcccgacaa cgaaaagccc
     2821 cggtaactgc acacgccccg gtaggcctgg tagttgtacc ggtcccgcaa catgttcatg
     2881 cgatagcagt cgccgtagcc gttggtcatc acgaggctgt aggcatgggg ttcggccggt
     2941 gccgcggccg gttcgttcag gagttccatc atgaggcgct gttcatccat cggcggtcct
     3001 gcgcgcccgg cgtccagcgg gccttcgtcc gccgcgtacg gcacgccgcc cgcgacgctg
     3061 aaaccgccgc tgtagacgta catcgccacg gtgcaactca tgtcgaacgg ataccagttc
     3121 tcgtagcccg ccatgacaga agagttgagc caccggaaga cgtcgttcag gaaatacatg
     3181 ttggtgcccc accgcaggga cgtggaaaca atggagaaat gctcgggcat ggaggacgtc
     3241 gccttgatgc gcagcatcac cacctcgccc gtcgtggagc agcccgacaa cgaaaagccc
     3301 cggtaactgc acacgccccg gtaggcctgg tagttgtacc ggtcccgcaa catgttcatg
     3361 cgatagcagt cgccgtagcc gttggtcaga atcaggctgt acgcggcccg ggtgtgttca
     3421 ggaaaaccga agaacccgag cagcgccacc ggcaagaagc cccatcgtcg caggatattc
     3481 acgccatctc ctcatgcgtc attcggcacg tccctgattg ttcgggcaga catcggagtt
     3541 aatgctttct gcctttcttg ataattaggg ggaaaaaagt cttttgtata tagaagattg
     3601 ctctgaaaaa ccctccgcta ttcccgggtt tcacaggttt gatggggaca gcccttacgt
     3661 gttaaatact gattacacac gatgtgcagc ctgcaaagaa aaagtcttgg atacccccag
     3721 aattcattga acgggctggg cacacctcat gccgtggtct cggggccaga gcggttggaa
     3781 ctgatcgtgc gcagtgtaaa gtcccttttg gttgtttcat gcagaggcag gaaagcgcag
     3841 caaggcgaag atgcctgcaa gagttccgca ccgaaaacac ctgtcattac tgctcaaaac
     3901 gctacgtctg ccgcatgaaa cagcgggaca aattggagcc agctcccgga ttcgaaccgg
     3961 ggacccactg attacaaatc agtagctctg ccactgagct aagctggctt gaatcgtccg
     4021 acgcggatgc agtctagcac gacgcggatc gcgcgcaaac catgcttcag ggcgtcgtcg
     4081 tttccggcaa ggcttctgcc gcgggcgctt cgatgggcgg cgcggggatc tccagccgcg
     4141 ccgcttcgac ggtgggatcc agaatggcag gcgtctccgc ctccggggcg gaagtcggcg
     4201 gcatcttcgg ggcgggcgcg gccgccgcct ttttcgcttc ggccgcctgt ctcttctgct
     4261 cggccctcgc ggcggcggcc tcggctttct tctggcgggc cagctcgcgg cgctccgcgc
     4321 gggtcagcgg tttcggcggc tcttcccggg gcgtgtccac ggacgtcgaa gcgggctccg
     4381 gggccggcgc ttccacggcc ggcggggcgg cgggggtctc gggcgcagcg ggcggttcga
     4441 cgggttgcgc gggacgggcc gccgcaggat gacgcgccgc cagcatctgg aacaggtatt
     4501 ccgccagcat ctcggaggcc gtcatcttca ccttctgctc gtgcgccatg atcgtggtgc
     4561 gcaggagccc cagcgcctgc ccgtaatgcg gggactgcgg atcgtacaac tcttccagcg
     4621 cgacgccctt ttcctcggca taggccacgt gcgcgctttc cgccagggtc aggatgtcca
     4681 caatcggccg cagggcctgg ttcttcatgt agtctgacgc ggccccgtcg gcatcgggcc
     4741 agtcgagctt gagctcactc gcctccatcg aggcggacac gccgcggaca atgatataga
     4801 aacgccggcc gtactggatc acggtgccaa ggaactggta gcggcggtcc agcggcaggc
     4861 cgttgaacag gttgaccagc tcggccgcca cgtagcactg gccggccacg gccgtggaaa
     4921 acgaggcata acgccggttg tcgagcttga ccagcccgcc ggacggaaga gcgcgcaggg
     4981 tatccgcaaa aacgaagctc cgggcccagt atttctgcgg gctgcgcacg agctcgacgg
     5041 cctcaacggg cttcgtctca tcctgggccg gggccggcgg ggcccacccg gctccgacca
     5101 gggccagcat gatcgagaga tattttctca tttccgcatc cttctccgcg ccctgcgcgc
     5161 cgcgtattcg tctgcttatc ccgcccccgc cccaaacgca aggaaaaccg ggccgccccg
     5221 ggacccgggg ctccctcaca ccttgcccgc cgacgggcac aggccgcgca cggggcagcg
     5281 cgggcattcc ggcttgcggg cataacaaca gcgccgcccg tggaatacca tcgcgtgcga
     5341 ccaggcgccc cagtccttct ccggcacgat ggccttgagg tcgaactcga tcttgtcggg
     5401 atcctcgtta tcggtgaacc ccaaccgcgc cgacagccgc ttgaaatgcg tgtccacgat
     5461 gatgcccggc cggcggaaag cggaaaccag cagcaggttg gcggtcttgc gcccgatccc
     5521 gggcagcgtg accagcgtgt cgaggtcctc gggcagccgg ccgccgaatt gcttcgccag
     5581 cgcggccccg agcgcccgga tgttcttcgc cttgttgcgg tagaacccgg tggaccggat
     5641 ctccttttcc agggtcgcct gcggcgtccg cgcccagtcg gccgccgtcc ggtatttctt
     5701 gaacagggcc ggcgtgacca ggttgacccg cttgtccgtg cactgggcgg acaggatcgc
     5761 ggccatgacc agttccaggg gattggagaa gtccagttcg cagtccgcgt cgggatattc
     5821 cgcgcgcagg cgccggagca cctcggcggc gcgcctttta agctgcggcg ttgccttgat
     5881 catccttcac cacgagccag aaacaaaaac cacgaaatac acggaccaca cgaaaagccc
     5941 ggaacgaatc cgcttgtttc gtgcgtttcg tgtatttcgt ggtcgttttt acgccttctt
     6001 gcggtcccgg atcaggactt ggcgtccttc tcctcgaggg cggccttgcg gctcaagcgg
     6061 atcttcccgc tctcgtccac gctcaagcac ttcacccaga cgaggtcgcc gaccttgacc
     6121 acgtcctcgg tgcgcttgac tcggaagttg gcgagctcgc tgatgtgcac caggccctcc
     6181 tggccgggca ggatctcgac gaacgcgccg aactccttca cgcccgtgac ggtgccctgg
     6241 tagatcttgc ccggctcggc ctcggccgtg accatcgaga cctcgcgcac cgccgcctcc
     6301 atcgcctcct tgtcggcgct gaagatgtgg acgctgccgt cgtcgtcg
//
LOCUS       DAOOTK010000157         6265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7719_length_6265_cov_4.900322, whole genome shotgun sequence.
ACCESSION   DAOOTK010000157 DAOOTK010000000
VERSION     DAOOTK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_6265_cov_4.900322"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PK634_12995"
     CDS             <1..636
                     /locus_tag="PK634_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98692.1"
                     /translation="ERPDMDAAIGCYRVKRHGWFRNLGSALIRALNRRAFNLPRDLCS
                     SSFRALRRPVVDSLVRWRTRNPALAAMIYQTTRRIVNVPVAHAVRFAGCSNYTLYRQA
                     RMALDYLCNVSLLPLHAMAVAGFGFCLFSVAFVIRTLHRYYADHIDVEGWTSLIIMVS
                     LSAGLILLSLGVIGEYIVRILREVQGAPRCQERQRAGWIAPARADNAGGGI"
     gene            744..1331
                     /locus_tag="PK634_13000"
     CDS             744..1331
                     /locus_tag="PK634_13000"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOW98693.1"
                     /translation="MSSIVTREAPDFAAQAVMPDNSITELRLSSHRGKYVILFFYPLD
                     FTFVCPSEIIAFDKAIQRFKEKNAVVIGVSVDSVHSHYAWRQLPRNKGGIGEIGFPLV
                     ADLNKKIARDYGVLLNEEVALRGLFLIDTHGIVRHALVNDLPLGRSVDEALRVLEALQ
                     FTEEHGEVCPANWQKGQEGMKPTAEGVAAYLGKHG"
     gene            1588..2736
                     /gene="lhgO"
                     /locus_tag="PK634_13005"
     CDS             1588..2736
                     /gene="lhgO"
                     /locus_tag="PK634_13005"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOW98694.1"
                     /translation="MALLEKPGRAVVVLEAEPRLAAHQTGHNSGVMHSGLYYKPGSLK
                     AQNCTTGREAMYRFCAERGIAHDRCGKIVVATSQEEIPALNRLEERGRANGLDGIRRL
                     APEAIRELEPHVTGVAGLHVPTTGIVDYTEVTETFARIVREGGGEIRTSARVAGVRRE
                     TGGLVLETAQGAVKARNLINCAGLQSDRMARLCGVNPGVRIVPFRGEYYELAPDKHHL
                     VRNLVYPVPDPQFPFLGVHFTRMVKGGVEAGPNAVLAFKREGYAKTSFSLRDTFETFT
                     YPGALRLFAKHWRMGLGEFYRSFRKRAFVRALQKLLPEIGMDDLEPGGAGVRAQAVGP
                     DGAPIDDFKIVEAERMVHVLNAPSPAATASISIGRSIAGMAERNFELK"
     gene            2790..3530
                     /locus_tag="PK634_13010"
     CDS             2790..3530
                     /locus_tag="PK634_13010"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HOW98695.1"
                     /translation="MQPSRVLIPLAFLFACFVGRAEEEIALIYNGPAADPYGAEAVAA
                     LAEKAGLDPVFFRSFRKLPDLLEDASVLIVGGTEDDLDPILREATPRVRKAVKEFIAA
                     GGRYLGICGGAYVASAGWPDTDRFVKALGLVAVKTDDFTDDDEPQIIRVQWKGRKRSL
                     YFQYGPQFLVDGRTAKTIHKTAFYSDGSLAALWMPSGKGRIYLCGPHPEADESWLEEE
                     DGSTIRNHSAWRDTDDLAEDMMNDLLAD"
     gene            3573..4733
                     /locus_tag="PK634_13015"
     CDS             3573..4733
                     /locus_tag="PK634_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOW98696.1"
                     /translation="MSSLDFGKKRFGATVRDWNDWRWQVRHAFRTRADLECLFPLSPE
                     EAAAFDGRPLRLPVAITPYYASLLNPASPLRRIVVPTAAETRRGAGEYDDPLGEEKHR
                     PVPCLVHTYPDRALFLVTGVCPAYCRYCTRARLAGRGAGLSSRANWRRALSYLREHAE
                     IRDVVISGGEPLLLPDEKLEWLLWELRHIPHLELLRISTRVPAVLPQRVTPALARLFG
                     KCQPLWVSVHFAHPDELTPEAARACARLAEAGVPMGSQTVLLRGINDSPDTIRRLMRG
                     LLRMRVKPYYLHQCDAVVGSAAFRASVQDGINILRALHGHTSGYAVPLYMIDAPGGGG
                     KVPLSPDYILGRKNGTLLLENYRGQRFRYRLADGDPFARGRRRKGPGAGLLA"
     gene            4783..>6265
                     /gene="secA"
                     /locus_tag="PK634_13020"
     CDS             4783..>6265
                     /gene="secA"
                     /locus_tag="PK634_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059246.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOW98697.1"
                     /translation="MLNWILKKIIGTKNERDVKLLRPLVARVNELEVEYQALSEEALK
                     AKTLEFKHRLAQGQTVDDLMCEAFAVVKNACRRMSGRTIQVCGRPLVWDMVPFDVQIL
                     GGIVLHQGKISEMATGEGKTLVATMPAYLNALTGENVNIVTVNDYLARRDSEWMGAVY
                     QYLGLTVGCIQNSMDPGERRIQYGRDITYGTNSEFGFDYLRDKGMAYRPEEQVQRGYF
                     YAIIDEVDSILIDEARTPLIISGPAPYSTTQYQELKSHVERLVRRQRDLCTRLITEAK
                     TLIEKGDDEEASRKLYQVHQGMPKNKQLQHLLEEPGPRRLLERINSSLLTDMRKDEAR
                     ALREELYFTIDEKGNDASLTEKGCEALSPNDPEMYVVPDLVSALSALDGDATLTDEEK
                     FKKRQEIQNLFADRSERIHAVDQLIRAYTVYEKDVQYVVQDNQVIIVDEFTGRLMAGR
                     RWSDGLHQAVEAKEGVTIERETQTLATITIQNYFRMYKKLAGMT"
BASE COUNT         1081 a         2125 c         2074 g          985 t
ORIGIN      
        1 gagcgcccgg acatggatgc cgcgatcggc tgctaccggg tcaagcgcca cggctggttc
       61 cgcaacctcg gctcggcgct gatccgcgcg ctgaaccgcc gcgccttcaa cctgccccgc
      121 gacctctgct cgtccagttt ccgcgcgctg cggcgcccgg tcgtggattc gctggtccgc
      181 tggcgcaccc ggaacccggc gctggcggcg atgatctacc agacgacgcg ccggatcgtg
      241 aacgtcccgg tcgcgcacgc cgtccggttc gcgggctgca gcaactacac gctgtatcgc
      301 caggcgcgca tggcgttgga ttacctgtgc aacgtgagcc tgctgcccct gcacgcgatg
      361 gccgttgccg gcttcggctt ctgcctgttc agcgtcgctt tcgtgatccg caccctgcac
      421 cgctactacg ccgaccatat cgacgtcgag ggctggacct cgttgatcat catggtctcg
      481 ctctccgcgg ggctcatcct gctctcgctg ggcgtcatcg gcgagtacat cgtgcgcatc
      541 ctgcgcgagg tgcagggcgc cccgcgctgc caggagcgcc agcgcgccgg gtggatcgct
      601 cccgcccgcg ccgacaacgc cggcggcgga atctgaccgg gccggcttga aaactcgcgt
      661 ttcgcatgta ccgtacccgc gctatgcatt caccatgagc cgggcgcgcc ccgcgcgccc
      721 aaaaccaggg aaaggaaaag aacatgtcgt ccatcgtcac ccgcgaggct ccggacttcg
      781 ccgcccaggc ggtcatgccg gacaactcga ttaccgaatt gcgcctgtcg tcccatcgcg
      841 gcaagtacgt gatcctgttc ttctacccgc tcgacttcac cttcgtgtgc ccgtcggaga
      901 tcatcgcgtt cgacaaggcg atccagcgct tcaaggagaa gaacgcggtc gtcatcggcg
      961 tctcggtgga ctccgtccac tcccattatg cgtggcggca actgccgcgg aacaagggcg
     1021 gcatcggaga gatcggcttc ccgctggtcg cggatctgaa caagaagatc gcccgcgact
     1081 acggcgtgct gctgaacgag gaagtcgcgc tgcgcgggct gttcctgatc gacacccacg
     1141 gcattgtgcg ccacgcgctg gtcaacgatc tgccgctggg gcggagcgtg gacgaggccc
     1201 ttcgcgtgct cgaggccctg caattcaccg aggagcacgg cgaggtctgc ccggccaact
     1261 ggcagaaagg ccaggagggc atgaagccga cggccgaggg cgtggcggcc tacctgggca
     1321 agcacggctg acgcctggaa aagcaggcgc gcggcgccgc ggcgcgctga taatatcgta
     1381 acaaaccgct tcacacccgt gaagcggttt gttgctttcc agggacggga cggcgggccg
     1441 ggggcaagaa tggccttgat gatcgggccg cgccgtgcca tgaaacctcc cccgacggca
     1501 caacccggca ggagaatacc cgtgcaagaa gcaaaaccct tcgacgtggc ggtgatcggc
     1561 ggcggcatcg tgggcgtggc gacggccatg gccctgctcg aaaagccggg ccgcgccgtc
     1621 gtggtgctgg aggcggagcc ccggctcgcc gcgcaccaga ccggccacaa cagcggcgtg
     1681 atgcattccg gcctctacta caagcccggc tcactcaagg cccagaactg cacgacgggc
     1741 cgcgaggcga tgtaccgctt ctgcgccgag cgcggcatcg cccacgatcg ctgcggcaag
     1801 atcgtggtgg ccacgtcgca agaggagatc ccggcgctga accggctgga ggagcgcggc
     1861 cgggcgaacg gcctggacgg catacgtcgg ctcgccccgg aagccatccg cgaactcgag
     1921 ccccatgtca ccggtgttgc cgggctgcac gtgccgacca cgggtatcgt ggattacacc
     1981 gaggtcacgg agacgttcgc acggatcgtc cgcgagggcg ggggcgagat ccgcacgtcg
     2041 gcgcgggtcg ccggcgtgcg ccgcgagacc ggcgggctgg tcctggagac cgcgcagggc
     2101 gcggtcaagg cccggaacct catcaactgc gccggcctgc agtcggaccg catggcccgg
     2161 ctctgcggcg tgaatcccgg cgtgaggatc gtcccattcc gcggcgagta ctacgaactc
     2221 gcgccggaca agcaccacct cgtccgcaac ctggtctatc cggtgcccga cccgcagttc
     2281 ccgttcctcg gcgtgcattt cacccggatg gtcaagggcg gcgtcgaggc gggcccgaac
     2341 gccgtgctgg cgttcaagcg cgagggctac gcgaagacca gtttctccct gcgcgacacg
     2401 ttcgagacgt tcacctatcc cggcgcgctg cggctgttcg cgaagcactg gcggatgggg
     2461 ttgggcgagt tctaccgctc attcaggaag cgggccttcg tacgggcgtt gcagaaattg
     2521 ttgccggaga tcgggatgga cgacctggag cccggcggcg ccggcgtccg cgcgcaggcc
     2581 gtcgggcccg acggcgcgcc gatcgacgac ttcaagatcg tcgaggccga gcgcatggtc
     2641 cacgtgctca acgccccgtc gcccgccgcg acggcctcga tcagcatcgg ccggtccatc
     2701 gcgggcatgg ccgagcgcaa ctttgagctg aagtgacggc cggggagatt cattatcgtc
     2761 cgcaacatcc gtttacaatg aagatcggca tgcaaccatc gagagttctc attccgctgg
     2821 cctttctctt tgcctgtttt gtcgggcgcg cggaggagga gatcgcgttg atctacaacg
     2881 ggccggcggc cgacccgtat ggcgcggagg ccgtcgcggc gctggcggag aaggcgggcc
     2941 tggaccccgt gttcttccgg agcttcagga aattgccgga cctgctcgag gacgcgtcgg
     3001 tcctgatcgt gggcggcacc gaggatgacc tggatccgat cctgcgggag gcgacgcccc
     3061 gggtcaggaa ggcggtgaag gagttcatcg cggccggtgg ccggtatctc ggcatctgcg
     3121 ggggggccta cgtcgcctcg gcggggtggc cggacacgga ccgattcgtc aaggcgctcg
     3181 gtctcgttgc ggtgaagacg gacgatttca cggacgatga cgaaccacag atcatccggg
     3241 tccagtggaa gggccggaag aggagcctct acttccagta cggcccccaa ttccttgtcg
     3301 acgggagaac ggccaaaaca atccacaaga cggccttcta ctccgacggc tccctggccg
     3361 cgttatggat gccttccggc aagggccgca tctacctctg cggtccgcat cccgaagcgg
     3421 acgagagctg gctggaggag gaagacgggt ccacgatcag gaaccattca gcttggcgcg
     3481 acacggatga tctggccgag gacatgatga atgatctgct cgcggactga tgcctcccgg
     3541 cggacttctg ctctcgattc ctgctattcc acatgagctc gcttgatttt gggaaaaaac
     3601 gattcggcgc gaccgtccgc gactggaacg actggcgctg gcaggtgcgg cacgccttcc
     3661 ggacgcgggc ggacctggaa tgtctgttcc ccctgtcgcc ggaagaggct gcggctttcg
     3721 atggccggcc gctccggctt cctgtggcca tcactccgta ttatgcttcg ctgttgaatc
     3781 cggcgtcgcc gctgcgccgg atcgtcgtgc ccaccgcggc cgaaacccgg cgcggggcgg
     3841 gggagtacga cgatccgctg ggcgaggaga agcaccggcc cgtgccgtgc ctggtgcata
     3901 cgtatcccga ccgcgcgctg ttcctggtca cgggagtctg cccggcctac tgccgctact
     3961 gcacgcgcgc acgcctcgcc ggccgcgggg cggggctttc ctcccgcgca aactggcgcc
     4021 gcgccctgtc ctacctccgg gagcacgcgg agatccgcga cgtggtgatc tcgggcggcg
     4081 agccgctgct gctgccggac gaaaaactgg aatggctgct ctgggagctc cggcatattc
     4141 cgcacctgga actgctgcgc atcagcacgc gcgtcccggc cgtgctgccc cagcgcgtga
     4201 cgccggccct cgcgcgattg ttcgggaaat gccagccgct gtgggtcagt gtgcatttcg
     4261 cgcacccgga cgagctgacg ccggaggcgg cgcgcgcgtg cgcccggctg gcggaggccg
     4321 gcgttccgat gggcagccag acggtgctgc tgcgcgggat caacgactcg cccgacacaa
     4381 tccgccggct catgcgcggc ctgctgcgga tgcgcgtgaa accctactac ctccaccagt
     4441 gcgacgccgt cgtcggctcc gccgccttcc gggcttccgt gcaggacggc ataaacatcc
     4501 tgcgcgccct gcacgggcac accagcggct acgccgtgcc gctgtacatg atcgacgcgc
     4561 ccggcggcgg gggcaaggtg ccgctgtcgc cggattatat tctcggccgc aaaaacggga
     4621 ccctgctgct ggaaaattac cgggggcagc gcttccggta ccggctcgcc gacggcgacc
     4681 cgtttgcccg gggccggcgg agaaaaggac cgggcgcggg gctgcttgca taaaaccgcg
     4741 ggccccctta ctctgtcgcc cggcccggaa aggggctttg ccatgctgaa ctggatcctg
     4801 aaaaaaatca tcggcacgaa gaacgagcgc gacgtgaagc tcctgcgccc gctggtcgcg
     4861 cgcgtcaacg agctggaagt cgagtaccag gccttgtccg aggaggcgct gaaggccaag
     4921 accctcgagt tcaagcaccg cctggcccag ggtcagacgg tggacgacct gatgtgcgag
     4981 gccttcgccg tggtcaagaa cgcctgccgc cgcatgagcg ggcgcacgat ccaggtctgc
     5041 ggacggccgc tggtctggga catggttccg ttcgacgtcc agatcctcgg cgggatcgtc
     5101 ctgcaccagg gcaagatttc cgaaatggcc accggggagg gcaagaccct cgtcgccacc
     5161 atgccggcct acctcaacgc cctgacgggc gagaacgtga acatcgtcac ggtcaacgat
     5221 tacctggcgc ggcgcgactc ggagtggatg ggcgccgtgt accagtacct cggcctgacg
     5281 gtcggctgca tccagaactc gatggacccg ggcgagcgcc gcatccagta cggccgcgac
     5341 atcacctacg gcaccaacag cgagttcggc ttcgactacc tgcgcgacaa ggggatggcc
     5401 taccgccccg aggagcaggt ccagcgcggc tacttctacg ccatcatcga cgaggtggac
     5461 tccatcctga tcgacgaggc ccgcacgccg ctgatcatct ccggccccgc cccgtattcc
     5521 acgacgcagt accaggagct caagtcgcac gtggagcggc tcgtgcgccg ccagcgcgat
     5581 ctctgcaccc ggctcatcac cgaggcgaag acgctcatcg agaagggcga cgacgaggag
     5641 gcctcgcgga agctctacca ggtgcaccag ggcatgccga agaacaagca gctccagcac
     5701 ctcctcgagg agcccggccc gcgccgcctg ctggagagaa tcaattcctc gctgctcacc
     5761 gacatgcgca aggacgaggc ccgcgccctg cgcgaagagc tctacttcac catcgacgag
     5821 aagggcaacg acgccagcct caccgaaaag ggctgcgagg ccttgagccc caacgacccc
     5881 gagatgtacg tcgtgcccga cctcgtctcg gccctctccg cactcgacgg cgacgcgacc
     5941 ttgaccgacg aggagaagtt caagaagcgg caggagatcc agaacctctt cgccgaccgc
     6001 agcgagcgca tccacgccgt ggaccagctc atccgcgcct acacggtcta cgagaaggac
     6061 gtccaatacg tggtccagga caaccaggtc atcatcgtgg acgaattcac cggccgcctg
     6121 atggccggcc gccgctggag cgacggcctc caccaggccg tcgaggccaa ggagggcgtg
     6181 accatcgagc gcgagaccca gaccctcgcc acgatcacga tccagaacta cttccgcatg
     6241 tacaagaagc tcgccggcat gaccg
//
LOCUS       DAOOTK010000158         6189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_7857_length_6189_cov_3.829475, whole genome shotgun sequence.
ACCESSION   DAOOTK010000158 DAOOTK010000000
VERSION     DAOOTK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7857_length_6189_cov_3.829475"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK634_13025"
     CDS             <1..489
                     /locus_tag="PK634_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98698.1"
                     /translation="KLAEMRESELSARVRLEQAERQLARLTEEQRRLEAELEQSGGSD
                     PRARAMTWMKAYQDLSARHDSLMAEFAAKTREIQEARETCARVEEETQLRLRHMESVV
                     QREREEAQQARKRLAEVEDSHLQLVKSYRELNDRYIRAREQAAPASASAPDSGPRIKL
                     TR"
     gene            complement(490..1332)
                     /locus_tag="PK634_13030"
     CDS             complement(490..1332)
                     /locus_tag="PK634_13030"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOW98699.1"
                     /translation="MKVAIIGAKGLLGADLSAACRRAGWDVVELGREQLDVTQPAIVR
                     KNLPPVPWVINCAAFSDIEAVEKQRARAFAVNSEGARAVAVSCARRGIRLMHISCAGV
                     FDGRKATPFREADEPRPVNAHGLSKLAGEKAVRAEGGKSLVVRLPVLFGSRGPGLPNS
                     LLAAVRARQFPLKVPAEEIVTPAYTRHVAEGLVKLLTLDVTGVVHVAPAGRCTWREFA
                     QVLADRLSPGAAIEEVPFALFYPLAERPLQTVLDAQRYRQWTGHALPGWRQGIEAWLA
                     DAKA"
     gene            complement(1371..1535)
                     /locus_tag="PK634_13035"
     CDS             complement(1371..1535)
                     /locus_tag="PK634_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOW98700.1"
                     /translation="MRMLKYIGRLIKEFFEFAWHNKAWWIVPIVLILLLLGFLIISGS
                     AVAPFIYTLF"
     gene            complement(1576..3405)
                     /locus_tag="PK634_13040"
     CDS             complement(1576..3405)
                     /locus_tag="PK634_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008276482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOW98701.1"
                     /translation="MNILGISAFYHDSAAALIRDGEIIAAAQEERFTRKKHDANFPVN
                     AVRYCLAEGGVHEGQLDAVAFYDKPINKFNRILETYLTVAPNGLQSFMMAVPIWLKQK
                     LWIPLDIELALEKCGMRAPKDMYFPEHHESHAASAFYPSPYESAAILTLDGVGEWATS
                     TLGRGRGNKIEIIKELRFPHSLGMLYSAFTYFTGFKVNSGEYKLMGLAPYGQPKYTQV
                     ILDNLLDLKEDGSFRLNMDYFGYLDRLTMTNEKFDGLFGGPPRKAESEISEREMDIAA
                     SIQKVTEEIVMRMARHAHQVTGEKDLCLAGGVALNCVANGRLLREGPFEDIWIQPAAG
                     DAGGALGAALLVWHMVHDKPRTAGGKRDKMKGAYLGPSFTDDEIQAFLDSRGYVYRKL
                     SDAEWAPTVAKLMAEEKVVGLLQGRMEFGPRALGGRSIIGDARSAKMQSVMNLKIKYR
                     ESFRPFAPTCLVEKVGDYFELDRPSPYMLLVAQVRRDRCKGIEESKTLNIRERINQVR
                     SDIPAITHVDYSARVQTVSREDNARYHDLIKAFDALTGYGVIINTSFNVRGEPIVCTP
                     EDAYRCFMRTEMDCLVLNSFVLEKGAQPPWKDSADWKSEYVLD"
     gene            complement(3405..3887)
                     /locus_tag="PK634_13045"
     CDS             complement(3405..3887)
                     /locus_tag="PK634_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98702.1"
                     /translation="MSTEKTAPVYSQNEVSAVVWPWCRAKASQDPAAAARALRHKALI
                     QAIVTAVVGAALYFLFKHALAAYIVWGIAAFVLVTGFLVQPVFRAMDRLMQRFGIAVG
                     TGLTYLLLVPFFYLVFMPGRLLMNVLGKDPLRLARATASPSCWSVRKPLKPDHFKKQF
                     "
     gene            complement(3988..4728)
                     /locus_tag="PK634_13050"
     CDS             complement(3988..4728)
                     /locus_tag="PK634_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98703.1"
                     /translation="MTMKTWAAGAALFLVIGTAGRAAILTETPEPLLESGGLERVSAG
                     VFYQWMERKVETERSYDMELDARTYEGVLGFDALPWLTLYAGAGGSDARFKDISAYGD
                     DQFSWALGFNMNIWQWVGYGEAPAWRLSFKTRGEIAGYESESRSLSIEWLDYSVAFPL
                     GYEVLFSRHPESFSDIDHLELFAGPALSFLDGRYEQGGQDIDFEEKHPFGVTVGGALF
                     ITETLFIELAGTYFDLWQVRGGIGYSFE"
     gene            complement(4797..4873)
                     /locus_tag="PK634_13055"
     tRNA            complement(4797..4873)
                     /locus_tag="PK634_13055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4837..4839),aa:Arg,seq:tcg)
     gene            4976..5533
                     /locus_tag="PK634_13060"
     CDS             4976..5533
                     /locus_tag="PK634_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOW98704.1"
                     /translation="MGGLSRKQVVRILYANLVLAVSVVSLAALLYLRRLHRFDALILE
                     AGRDYGVDPRLISAVIWVESRFREERVGKAGEIGLMQVTRTAAEEWAAENHDGLFTVL
                     DLFEPRVNIRAGTWYLARGLQRWSDRPDPLPFALAEYNAGRSNALRWSRDGGPDARAF
                     LDAITYPTTRRYIRDILKRYRGRAY"
     gene            5597..>6189
                     /locus_tag="PK634_13065"
     CDS             5597..>6189
                     /locus_tag="PK634_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98705.1"
                     /translation="MIVLKKEQEATLARPRMAYKTTARMLFWGMDFFYGKALTLRKVQ
                     VLEILARIPYQAWEIRQYLRLTHLYEDPASVEKGRKITAWARDAQDNEFWHLLVINER
                     MKQTGFRKSWFWDHVLPRVAAFQYRLFSRLLSLVNIKAAFDLNAQFEDHAEHEYMQFV
                     KDHPELDQEKVDHPIVREHGNCETWGDVFRRIGLDER"
BASE COUNT         1071 a         2089 c         2004 g         1025 t
ORIGIN      
        1 aaactggccg agatgcgcga gagcgagctg tccgcccgcg tccgcctgga gcaggcggag
       61 cgacagctgg cccgattgac cgaggagcag cggcgcctcg aagcggaact ggagcagtcc
      121 ggcggcagcg acccccgggc ccgcgccatg acgtggatga aggcctacca ggacctctcc
      181 gcgcgccacg attcgctcat ggccgagttc gcggccaaga cccgcgaaat ccaggaagcc
      241 cgcgagacgt gcgcgcgcgt cgaggaggag acccaactgc gcctccggca catggaatcg
      301 gtcgtccaac gggagcgcga ggaggcccag caggcgcgca agcggctggc cgaggtcgag
      361 gattcgcacc tgcagctcgt gaaatcctat cgggaattga acgaccgcta catccgcgcc
      421 cgcgagcagg ccgcgccggc ctccgcttcc gccccggact ccgggccgcg catcaagctg
      481 acgcgctgat caggccttgg cgtccgccag ccaggcctcg atcccctggc gccatcccgg
      541 cagcgcgtgg cctgtccact ggcggtaccg ctgcgcatcc agcacggtct ggagcgggcg
      601 ctcggccagc gggtagaaaa gggcgaaagg cacctcctcg atggccgcgc cggggctcaa
      661 cctgtccgcc aggacttggg cgaattcccg ccacgtgcag cgccccgccg gggccacgtg
      721 caccacgccg gtcacgtcca gcgtcagcag cttgaccagg ccctcggcca cgtgacgggt
      781 ataggcgggg gtgacgatct cctcggccgg caccttcagg gggaattgcc gtgcgcgcac
      841 ggcggccagc aggctgttcg gcagcccggg cccccggctt ccgaacagca cgggaaggcg
      901 cacgactagg gacttgccgc cctcggcgcg caccgccttc tcgccggcca gcttgctcaa
      961 cccgtgggcg ttgaccggtc ttggctcgtc ggcctcccgg aacggcgtgg ccttgcgccc
     1021 gtcgaacacc ccggcgcagc tgatgtgcat cagccggatc ccgcgccggg cgcaactgac
     1081 ggccaccgcc cgcgcgcctt cgctgttgac cgcgaacgcc cgcgcgcgct gtttttccac
     1141 ggcctcaatg tcgctgaagg cggcgcagtt gatcacccag ggaacgggcg gcaggttctt
     1201 gcggacgatg gccggttgtg tgacgtccag ctgctcgcgg cccagttcga cgacatccca
     1261 cccggcgcgc cggcaggccg ccgacaggtc ggcgcccagc aggcccttcg caccgatgat
     1321 cgccaccttc atatcgggag gcgcccccct gcccggccgg cggcggccgg tcagaacagg
     1381 gtgtagatga acggcgcgac ggcggacccg gaaatgatga ggaatccgag caggaggagg
     1441 atcaggacga tgggcacaat ccaccaggcc ttgttgtgcc aggcaaattc gaagaactcc
     1501 ttgatcagcc gcccgatgta tttcagcatg cgcatggtcg ttccatccct gtttcgccgc
     1561 gcccgcccgg gcgctctagt ccagcacgta ctcgctcttc cagtccgccg aatccttcca
     1621 gggcggctgg gcgcccttct ccagcacgaa cgaattgagg accaggcaat ccatctcggt
     1681 ccgcatgaag cagcggtacg cgtcctccgg cgtgcacacg atcggctctc cgcggacgtt
     1741 gaaggaggtg ttgatgatca cgccgtaacc ggtgagcgcg tcgaaggcct tgatcaggtc
     1801 gtggtagcgg gcgttgtcct cgcgggagac cgtctgcacg cgcgcggaat aatccacgtg
     1861 cgtgatggcc gggatatccg accgcacctg gttgatccgc tcgcggatgt tcagcgtctt
     1921 gctctcctcg atccccttgc agcggtcgcg gcgcacctgg gcgacaagca gcatgtacgg
     1981 cgaggggcgg tcgagctcga agtaatcgcc gaccttctcc acgaggcagg tcggcgcgaa
     2041 cggccggaac gactcgcggt acttgatctt gaggttcatc accgactgca tcttggccga
     2101 ccgggcgtcg ccgatgatcg agcggccgcc gagcgcgcgc ggcccgaact ccatgcggcc
     2161 ctgcagcagg ccgacgacct tctcctcggc catcagcttc gcgacggtcg gggcccactc
     2221 ggcatcggat agtttccggt agacatagcc gcgcgaatcg aggaaagcct ggatctcgtc
     2281 gtccgtgaag gagggcccga ggtacgcgcc cttcatcttg tcgcgctttc cgccggcggt
     2341 ccgcggcttg tcgtggacca tgtgccagac gagcagcgcc gcgccgagcg cgccgccggc
     2401 gtcgccggcc gcgggctgga tccagatgtc ctcgaacggc ccctcgcgga ggaggcggcc
     2461 gttggccacg cagttcagcg ccacgccgcc ggccaggcag aggtccttct cgcccgtgac
     2521 ctggtgcgcg tgccgggcca tccgcatgac gatctcctcg gtgaccttct ggatcgacgc
     2581 ggcgatatcc atctcgcgct cgctgatctc ggactccgcc ttgcgcggcg ggccgccgaa
     2641 caagccgtcg aacttctcgt tggtcatcgt cagccggtcg aggtacccga agtagtccat
     2701 gttcaggcgg aaggagccgt cctctttcag gtccagcagg ttgtcgagga tgacctgggt
     2761 gtacttcggc tgcccgtaag gcgcgaggcc catgagcttg tactcgccgg agttgacctt
     2821 gaaccccgtg aagtacgtga aggccgagta cagcatgccg agggagtgcg ggaaccgcag
     2881 ttccttgatg atctcgatct tgttcccgcg gccgcggccc agcgtgctgg tggcccactc
     2941 gcccacgccg tcgagcgtga ggatggcggc gctctcgtag ggggacgggt agaacgcgct
     3001 ggcggcgtgc gactcgtggt gctccgggaa gtacatgtcc ttcggggcgc gcatgccgca
     3061 tttttccagc gccagctcga tgtccagcgg gatccacagc ttctgtttca gccagatcgg
     3121 cacggccatc atgaacgact ggaggccgtt gggcgcgacg gtcaggtacg tttccaggat
     3181 acggttgaac ttgttgatgg gcttgtcgta gaacgcgacg gcgtccaact ggccctcgtg
     3241 caccccgcct tccgccaggc agtaccggac cgcgttgacc gggaagttgg cgtcgtgctt
     3301 cttgcgggtg aaccgctcct cctgggcggc ggcgatgatt tcgccgtcac ggatgagggc
     3361 ggcggcggaa tcgtggtaaa acgcgctgat gcctaggatg ttcattagaa ttgcttcttg
     3421 aagtgatcgg gtttcaacgg cttgcgaaca gaccagcaac tggggctcgc ggtcgcgcgg
     3481 gccaaccgca ggggatcctt gcccaggaca ttcatgagca ggcgcccggg catgaacacg
     3541 aggtagaaaa acgggaccag cagaagatag gtcagcccgg tgcccaccgc gattccgaac
     3601 cgctgcatga gccggtccat ggcgcggaag accggctgga ccagaaagcc ggtgaccagc
     3661 acgaaggcgg cgatccccca cacgatgtaa gccgccaggg cgtgcttgaa aaggaaatag
     3721 agcgcggccc cgacgaccgc tgtaacaata gcctgaataa gggctttatg gcgaagcgcc
     3781 cgggcggcgg ccgccgggtc ctggctcgcc ttcgcccggc accatggcca gacgacggcg
     3841 cttacctcgt tttgcgaata gaccggcgcg gttttttcag tgctcatgat cgggttccca
     3901 tcttccagtg cggcggaaag tgacgcatcc tgcgcccgta atcaagccca tttttgacgg
     3961 gcggccggcc ctccccgcat ccggcgctca ctcaaaactg tacccgatgc cgccgcgcac
     4021 ctgccacaaa tcgaagtatg ttccggccag ttcgatgaaa agcgtctcgg tgatgaacag
     4081 ggccccgccc accgtcaccc cgaagggatg cttttcctcg aaatcgatgt cctgaccgcc
     4141 ctgctcgtac cggccgtcga ggaaggacag ggccggcccc gcgaacagtt ccaggtgatc
     4201 gatgtccgag aagctttccg ggtggcggga gaacaagact tcgtatccga gcgggaacgc
     4261 caccgagtaa tccagccact cgatcgacag cgagcgggat tcggattcgt agcccgcgat
     4321 ctcgccgcgg gtcttgaagg aaagccgcca ggccggcgcc tccccgtagc ccacccactg
     4381 ccagatattc atgttgaacc cgagggccca cgagaactgg tcgtccccgt acgcgctgat
     4441 atccttgaag cgcgcgtccg agcctccggc cccggcgtac agggtcagcc acggcagcgc
     4501 gtcgaacccc agcacgccct cgtaggtgcg ggcgtccaac tccatgtcgt agctacgctc
     4561 tgtttccacc ttccgttcca tccactggta gaacacgccg gcgctgaccc gttccaggcc
     4621 gccggattcc agcaggggtt ccggggtttc ggtcaggatg gccgcccgcc ctgccgtgcc
     4681 gatcaccaga aacagcgctg cgcctgccgc ccatgtcttc attgtcatat ggtcacccat
     4741 catcgcgtcc cagcagactg acatccgccg cgcccgcccc gttcgattgg atcgcctggc
     4801 acacccggag ggatttgaac ccccaaccct cggatccgaa gtccgatgct ctgtccaatt
     4861 gagctacggg tgcccgcgag ggttgtagca ggaggcggga agcgggtcaa ccgccggacc
     4921 gtccggcgcg aggccgggat ggacgccggg ccggaaatgt cctagggtgg cgaccatggg
     4981 cggtctctcg cgaaaacagg tggtccgcat cctctacgct aatctcgttc tcgccgtgtc
     5041 cgtggtcagc ctcgcggcgc tgttgtatct ccggcgcctt catcggttcg acgcgctgat
     5101 cctcgaggct ggccgcgact acggcgtgga cccgcgcctg atctccgccg tgatctgggt
     5161 cgaaagccgg ttccgggaag aacgcgtggg caaggccggc gagatcggcc tgatgcaggt
     5221 gacgaggacc gccgccgagg agtgggccgc ggaaaaccat gacggcctct tcacggtgct
     5281 ggacctgttc gaaccgcgcg tgaacatccg ggccggcacc tggtacctgg cccgcggcct
     5341 ccagcgatgg tccgaccggc ccgacccgct ccccttcgcc ctcgccgaat acaatgccgg
     5401 acgctcgaac gcgctgcgct ggtcgcgcga cggcggcccc gacgcccggg cgttcctcga
     5461 cgccatcacc tatcccacca cgcgccggta catccgcgac atcctcaagc ggtatcgggg
     5521 ccgggcgtat tgatgacttg cgcggtcccg cgggccgggc tatactgcga ccggttcagc
     5581 caaaacggag gccgacatga tcgttttgaa aaaagagcag gaagccacgc tggcccggcc
     5641 gcgcatggcc tacaagacca cggcgcgcat gttgttctgg ggcatggatt tcttctacgg
     5701 gaaggccctg accctgcgca aggtgcaggt cctcgaaatc ctcgcccgca tcccctacca
     5761 ggcctgggaa atccgccagt atctccgcct gacgcacctc tacgaggatc cggcgagcgt
     5821 ggagaaaggc cggaaaatca cggcctgggc ccgcgacgcg caggacaacg agttctggca
     5881 cctgctggtg atcaacgaac gcatgaagca aaccggtttc cggaagagtt ggttctggga
     5941 tcatgtcctg ccccgcgtcg cagccttcca gtaccggctc ttcagccgcc tgctctccct
     6001 ggtgaacatc aaggccgcct tcgatctgaa cgcccagttc gaggaccatg ccgagcacga
     6061 gtacatgcaa ttcgtcaagg accaccccga actcgaccag gagaaagtgg accaccccat
     6121 cgtgcgcgag cacggaaact gcgaaacctg gggcgacgtg ttccggcgga tcgggctgga
     6181 cgagcgcga
//
LOCUS       DAOOTK010000159         6062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8070_length_6062_cov_4.176960, whole genome shotgun sequence.
ACCESSION   DAOOTK010000159 DAOOTK010000000
VERSION     DAOOTK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8070_length_6062_cov_4.176960"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..358
                     /locus_tag="PK634_13070"
     CDS             122..358
                     /locus_tag="PK634_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98706.1"
                     /translation="MERGAKALSIMLLLALALGAVIIAVNPMYRQAAIAMWKGEPEKS
                     PIWISNSEYYPDVALEGRPASPSPEEDQEAEVLP"
     gene            355..3810
                     /locus_tag="PK634_13075"
     CDS             355..3810
                     /locus_tag="PK634_13075"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOW98707.1"
                     /translation="MIQNKIIVFCYAYLLGIGLCMLAPLDVHFLPWLWLSVAIAFAFL
                     VAQSYRSRLRAAVDPAPSQRGLWIGLVVAPLLLGYTRYVSVNTLPDSTLGMLEIRDGQ
                     PSFVATMALNDTSRLRLEKTEPLSADLRVRFVGEVRSRVPILSEQGIPTLDSEARWRF
                     REVVTPQVSEVVEIKQNDPVGTSYVIPQPFNKIERVEVLSGPAAGRVKVHRVSNHIAS
                     FVRPGRNQTPVTLLARITSDPWLYSFKTVLTITPYYIQYRPDGPFFKVEGGDVRVTLG
                     PDSDNYTQFACTKSYGYDIVVHGELTMASGKANPAGFDQRKYLNNYGVFGQMSVKQFL
                     GGPRPLEAVAPAGQDLRTGNPLVELSLEIRDRLLRVFKQTHPYPSSAFMGGIALGLRY
                     GLQSTECMYSSMNPSSGSRSLVGQHDDCRELIADEFRVAGVNHVLAVSGLHVTIITVM
                     FVGIFTLLRMSRRVYVPIIVLALVIFAILTGARPSSLRAVIMNSLFLLTWAYMDKSLR
                     SSVLLGVPVAALLILLENPNVMVDPSFSLSFMAILALALLTGPSYDVLCKFKGNDLVS
                     LFLVIAFWTSSVIASWHLMESGTYWLLFVAFAGAVFGLGRLLTRLGIRLIGDRGFADI
                     PIGISTFIAAQFAIQLGMMVPLSPFYFCRWPVAGAFANLIAIPLVGVVLQVGVIAAMI
                     GLIPGIGIWIALLLNAADWVFGAGFLLIAHYCTKWFPYPLVARPTSRGLLLYFLLLAL
                     FVWWRPFYRWASDVVRARTRAGRARLGALVVLLVALFMVWVRYPVTWAEKSGLNITVL
                     AVRYGSSILIQTPGGKNVLVDAAFVQRDRSRANEAERNIIVYLTTLGIRRLDGLILTS
                     PRLERSSGIPYVLDRCRVDTFYTPPGLANLSTNITPEDFAAELGVPLEDTGALYPEII
                     GTPSAPRQLSTAKVLAGRGPTLVNRLAHAVIQHKTARAGDVLFREEVRGREFAIEVLS
                     PDAEPFAEHNLDNQSLVLRVRYGDFAMLLPSDLHFAGQQRLLERYDAAALRSDVVVMP
                     NRGVAMPGSFMVNMKEMVRSAMRTVTLPLFQKIAPKYAIFEFGNPKPVLGDLSRDSIK
                     VYEIARREICDALGDDRVLNTDRDLTIFIRSDGTSCEVVTQAEKLRGTASEDFGSVDE
                     MYDVP"
     gene            3823..4551
                     /locus_tag="PK634_13080"
     CDS             3823..4551
                     /locus_tag="PK634_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOW98708.1"
                     /translation="MNNAAQTPTGSPARCSKGLVVGLVIGVLALAAVAALFVVKSRRK
                     PVAIPAVRVETPAPVAAGTPAAVPAAAPVVAPVTAASAGEPLRVGGRYSGVVEDVGKQ
                     GDGIVKVQGKVVFVTGGRKGDRVEFEVTENLDRFARGRVVGMAAAAAPAAAAAADAPP
                     VDGATATAEEVQPGRVFDVMISEADRRAPDRDGVAKIGGLVVFVSNARPDERVRIRVV
                     QRMTRFARAEVIARPDAAASEAAP"
     gene            4553..5401
                     /locus_tag="PK634_13085"
     CDS             4553..5401
                     /locus_tag="PK634_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOW98709.1"
                     /translation="MRVIRSQSSLIGRNLAIEEWLLAPSAPPPPLLFLWTASPAVVIG
                     KNQNPWMECAPGHLETRGIRLARRISGGGAVYHDPGNLNYAFILDRASYRRETAYDGL
                     LARLRSLGIPAERLGRSGVGVHGRKFSGTAFCYRGERVLHHGTLLVSADLAELERALT
                     PPPVAIRGRAVPSERSPVVNLSDVAPGLTIDRLADVLADGLEPLPLESANAKAIGKLE
                     AKQASREWVYESTPPFEAEVLSWTLRVEKGWVALGPLAGCPFGSRDLAERLDGEDQSR
                     ILALGL"
     gene            5401..>6062
                     /locus_tag="PK634_13090"
     CDS             5401..>6062
                     /locus_tag="PK634_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98710.1"
                     /translation="MKHASHIAFAIFAAWLLGCGLAGRPVLHGDAREYILQTQAIALR
                     GQLQIEPARAAAYWNRTNPYGLELGETRPPSWPLNESAQAGGGFGALYPDRFGAWRFA
                     HFWAYSAVVAPLYGLLHLAAPAAEYAAFRIVNAAFLLLPFLLAWRRGGNGRLLIVMAL
                     AVFSPLIPYTDWAHPELFCFALVLASFQLASGPRGRWFAPALLGLAATQNPPILLFFP
                     LLL"
BASE COUNT          873 a         2085 c         2016 g         1088 t
ORIGIN      
        1 ttttgtgtac ttgtcgctgt ttcgagggca attgagagga cgggacgaag tgccggatct
       61 ggatcaggat cggaaccaga cgcggcgcta gcccgggctc ggtttaaaga gaggtgctgt
      121 catggaaaga ggcgcaaagg cattgtccat catgctgctg ctggcgctgg cgctcggggc
      181 ggtgatcatc gccgtcaatc ccatgtatcg ccaggccgcg atcgccatgt ggaagggcga
      241 gccggagaag tcgcccatat ggatatcgaa cagcgagtat tacccggatg tggccctgga
      301 aggccggccc gcctcgccgt ctccggagga agaccaggaa gccgaggtgc ttccatgatc
      361 cagaacaaga tcatcgtttt ctgctacgcc tacctcctcg gcatcgggct ctgcatgctc
      421 gcgccgctgg acgtgcattt cctgccctgg ctatggctgt cggtcgctat cgcttttgcc
      481 ttcctggtcg cgcagagcta ccggagccgg ctccgggcgg cggtggaccc ggcgccgtcc
      541 cagcggggcc tgtggatcgg gctggtcgtg gcccccctgc tgctgggtta cacgcgctat
      601 gtcagcgtca atacgctgcc ggactccacg ctgggcatgc tggagatccg cgacggccag
      661 ccgagtttcg tggccacgat ggccctgaac gacacgtccc ggcttcgcct ggagaagacg
      721 gagccgctgt ccgcggacct gcgggtgcgc ttcgtcggcg aggtgcgatc ccgcgtgccc
      781 atcctgtcgg agcagggcat cccgaccctg gacagcgagg cgcgctggcg gttccgggaa
      841 gtggtgacgc cgcaggtcag cgaggtcgtc gagatcaagc agaacgaccc ggtcggcacg
      901 agctacgtga tcccgcaacc gttcaacaag atcgagcggg tggaagtgtt gagcgggccg
      961 gccgcggggc gggtcaaggt ccaccgcgtc tccaatcaca tcgcctcctt cgtgcggccc
     1021 ggccggaacc agaccccggt gacgctcctg gcccgcatca cgtcggatcc ctggctctat
     1081 tcgttcaaga ccgtcctgac gatcacgccg tactacatcc agtaccggcc cgacggaccg
     1141 ttcttcaagg tggagggcgg ggacgtccgg gtcacgctgg gcccggactc ggacaactac
     1201 acccagttcg cgtgcaccaa atcctacggc tacgacatcg tcgtgcacgg cgaactgacc
     1261 atggccagcg gcaaggccaa tcccgccggc ttcgaccagc ggaagtacct caacaactac
     1321 ggcgtcttcg ggcagatgag cgtgaagcag ttcctcggcg gaccgcggcc gctcgaagcc
     1381 gtcgcgccgg caggccagga cctgcgcacc ggcaatcccc tggttgagtt gtcgctcgaa
     1441 atccgcgacc gcctgctgcg ggtgttcaag cagacgcatc cctatccgag ttcggccttc
     1501 atgggcggca tcgcgctggg cctgcgctac gggctgcaga gcaccgagtg catgtacagc
     1561 agcatgaacc cgtcctccgg ctcccggagc ctcgtcggcc agcacgatga ctgccgcgaa
     1621 ctcatcgccg acgagttccg cgtggcgggc gtgaaccacg tcctggccgt gtccggcctg
     1681 cacgtgacca tcatcacggt catgttcgtc ggcatcttca cgctgctgcg catgtcgcgg
     1741 cgcgtgtacg tgccgatcat cgtgctggcc ctggtcatct tcgccatcct caccggcgcc
     1801 cggccgtctt cgctgcgcgc cgtgatcatg aacagcctgt tcctgctgac ctgggcctac
     1861 atggacaaaa gcctgcgctc gtccgtcctg ctgggcgtgc ccgtggccgc gctcctcatc
     1921 cttctcgaga atccgaacgt gatggtcgac ccctccttca gcctgtcctt catggccatc
     1981 ctcgccctcg ccctgctcac cgggccgtcg tacgacgtgc tctgcaagtt caagggcaac
     2041 gacctggtca gcctgttcct ggtcatcgcc ttctggacgt cgtcggtgat cgcctcctgg
     2101 cacctcatgg aatccgggac gtactggctg ctgttcgtgg cgttcgcggg cgccgttttc
     2161 gggctcggac gcctgctgac gcgcctcggc atccggctga tcggcgaccg gggcttcgcc
     2221 gacatcccga tcggcatctc gacgttcatc gccgcccagt tcgccatcca gctgggcatg
     2281 atggtgcccc tgtccccatt ctacttctgc cgctggccgg tggccggcgc cttcgccaac
     2341 ctgatcgcca tcccgctcgt gggcgtggtg ctgcaggtgg gcgtgatcgc ggcgatgatc
     2401 gggctgatcc cgggcatcgg catctggatc gctctgctgc tgaacgcggc cgactgggtc
     2461 ttcggcgcag gcttcctgct catcgcccac tactgcacca agtggttccc gtaccccctc
     2521 gtcgcgcggc ccacctcgcg cgggctgctc ctgtatttcc tgctgctggc gctgtttgtc
     2581 tggtggcggc cgttctaccg gtgggcgtcc gatgtggtgc gggcgcgcac gcgcgccggc
     2641 cgcgcgcgcc taggagcgct cgtggttctg ctcgtggcct tgttcatggt ctgggttcgc
     2701 tacccggtca cctgggctga aaagtcgggc ctgaacatca ccgtactggc cgtccgctac
     2761 ggcagcagca tcctcatcca gacgcccggc gggaagaacg tcctggtgga cgccgccttc
     2821 gtccagcgcg accggagccg cgccaacgag gcggaacgca acatcatcgt ctacctgacc
     2881 acgctgggga tccgccgcct ggacggcttg atcctgacct cgccgcgcct cgagcggtcg
     2941 tcaggcatcc cgtacgtcct ggaccgctgc cgcgtggaca ccttctatac cccgccgggc
     3001 ctcgcgaacc tgtccaccaa catcaccccg gaagacttcg ccgcggagct cggcgttccc
     3061 ctcgaggata ccggcgcgct ctatcccgag atcatcggca cgccgagcgc gccgcgccag
     3121 ctctctaccg ccaaggtgct ggccgggcgc ggcccgacgt tggtcaaccg cctggcccac
     3181 gccgtcatcc agcacaagac cgcccgcgcc ggagacgtcc tgttccgcga ggaagtccgg
     3241 ggccgcgagt tcgccatcga ggtgctctcg cccgacgccg agcccttcgc cgagcacaac
     3301 ctggataacc agtcgctggt cctgcgcgtg cgctacggcg atttcgccat gctgctgcct
     3361 tcggacctcc atttcgccgg ccagcagcgc ctgctcgagc gctacgacgc ggcggcgctc
     3421 cgctccgacg tggtcgtgat gcccaaccgg ggcgtggcca tgcccgggag cttcatggtc
     3481 aacatgaagg agatggtgcg ctcggcgatg cggaccgtca cgctgcccct cttccagaag
     3541 atcgcgccga agtacgcgat cttcgagttc ggcaatccca agcccgtgct gggcgacctg
     3601 tcccgcgaca gcatcaaggt ctacgagatc gcgcgccggg agatctgcga tgccctgggc
     3661 gacgaccggg tcctcaatac ggaccgcgac ctgacgatct tcatccgctc ggacggaacg
     3721 tcctgcgagg tcgtcacgca ggcggaaaag ctgcgcggca ccgcgtccga ggatttcggg
     3781 agcgtggatg agatgtacga cgtgccctga tcggaggctg ccatgaacaa cgccgcgcag
     3841 acacccaccg gatcgcccgc acgctgctcg aaagggctcg tggtcggatt ggtcatcggc
     3901 gtgctcgcgc tcgcggcagt ggcggcgctg ttcgtggtca aaagccgcag gaaaccggtg
     3961 gccattccgg cggtccgcgt ggaaacccct gcgcctgtcg ccgccgggac accggcagcg
     4021 gtgccggcgg ccgcgccggt tgtcgctccc gtgacggccg cgtccgcagg ggaaccgctg
     4081 cgcgtgggag ggcggtattc cggcgtcgtc gaggatgtgg gcaagcaggg cgacgggatc
     4141 gtgaaggtgc agggcaaggt ggtgttcgtc accggcgggc ggaagggcga ccgggtggag
     4201 ttcgaggtca ccgagaacct ggaccgcttt gcccgaggac gcgttgtcgg gatggccgcg
     4261 gccgccgccc ctgcggccgc cgccgcggcg gacgcgccgc ccgtggacgg cgccaccgcg
     4321 acggccgagg aggtccagcc cggccgcgtc ttcgatgtca tgatcagcga ggcggatcgc
     4381 cgcgcccccg atcgcgacgg ggtcgccaag ataggcgggc tggtcgtgtt cgtgtcgaat
     4441 gcgcgtcccg acgagcgcgt ccgcatccgg gtggtgcagc gcatgacgcg attcgcgcgg
     4501 gccgaagtca tcgcccgccc cgatgccgcc gcttcggagg ccgcgccctg aattgcgggt
     4561 catccgatcc cagtcctcgc tgatcgggcg aaacctcgcc atcgaggaat ggctactcgc
     4621 tccgtccgcg cccccgccgc ccctgctgtt cctgtggacg gctagccccg ccgttgtcat
     4681 cggaaagaac cagaatccct ggatggaatg cgccccggga catctggaga cgcgggggat
     4741 tcggctggcc cggagaatat ccggcggtgg cgcggtgtat cacgatccgg gcaatctcaa
     4801 ttacgccttc atcctcgatc gcgcctccta tcgccgcgag accgcctacg atgggctcct
     4861 ggcgcggctg cggtctctgg ggatcccggc ggaacgcctg ggacggagcg gggtgggggt
     4921 gcatggccgc aagttctccg gcacggcctt ctgctatcgc ggggagcgcg tgctgcatca
     4981 cggcacgctg ctggtttccg ccgatctggc ggaactggag cgcgcgctga caccgccgcc
     5041 cgtcgcgatc cggggccgcg ccgtcccgtc cgagcggtcg ccggtcgtga acctgtccga
     5101 cgttgcgccc ggcctgacga tcgaccggct ggccgatgtg ctggcggacg gcctggaacc
     5161 gctgccgctc gagtccgcga acgcgaaggc catcgggaaa ctggaagcca aacaggcatc
     5221 gcgcgagtgg gtgtatgaat ccacgccgcc gttcgaggcg gaggtgctgt cctggaccct
     5281 gcgcgtggag aaggggtggg tggcgttggg gcccttggct ggatgcccgt tcgggtcgcg
     5341 ggacctggcg gaacggttgg acggggaaga ccagtcgcgt atcctggcgc tgggccttta
     5401 gtgaaacacg ccagtcatat tgcctttgct atatttgccg catggctgct gggctgcggc
     5461 ctggcgggcc gtcccgtcct gcacggcgat gcgcgggagt acatcctgca gacgcaggcc
     5521 atcgcgttgc gcgggcagct ccagatcgag cccgctcgcg ccgccgcgta ctggaaccgg
     5581 acgaatccgt acggcctgga actgggcgag acgcgcccgc cgtcctggcc gctgaacgaa
     5641 agcgcgcagg ccggcggcgg cttcggcgcg ctctaccccg accgctttgg ggcctggcgc
     5701 ttcgcgcatt tctgggccta ctccgccgtt gtggcgccgc tctacggtct tcttcacctc
     5761 gcggcgccgg cggccgaata tgccgccttc cggatcgtta acgcagcctt tctcctgctc
     5821 cctttcctcc tcgcctggcg acgaggggga aacgggcggc tgctgatcgt catggccctg
     5881 gcggtgtttt cgccgctgat cccgtacacc gactgggcgc acccggagtt gttctgtttc
     5941 gcgctggtcc tggcctcttt ccaactcgcg agtgggccgc gcggacgctg gttcgccccc
     6001 gcgctgctcg gactcgcggc cacgcagaac ccgccgatcc tgcttttctt tccgctgctc
     6061 ct
//
LOCUS       DAOOTK010000160         5998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8200_length_5998_cov_5.538280, whole genome shotgun sequence.
ACCESSION   DAOOTK010000160 DAOOTK010000000
VERSION     DAOOTK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8200_length_5998_cov_5.538280"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK634_13095"
     CDS             complement(<1..420)
                     /locus_tag="PK634_13095"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98711.1"
                     /translation="MSDDPAPASPPDDDLRAQQYEAIHNRLFLLRLALTLGLLWAYLL
                     TGASTLLAEGLRARFGGWWPVVNALYTLITLFGFSALMFPLSYFGDYAVERRYGLSHQ
                     TPEGWFLDYLKSLGLELLLGIVFFSAIYAFLHFLPGSW"
     gene            complement(431..1336)
                     /locus_tag="PK634_13100"
     CDS             complement(431..1336)
                     /locus_tag="PK634_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOW98712.1"
                     /translation="MAAKHIGLGRGLSALIKDTPPPEPAPAAEGERGIVKLPVDQIHK
                     SPWQPRHEMRPEAIEDLAHSIREHGVLQPLLVRRVEDHYELIAGERRLRAAQAAELRE
                     VPARIMEVSDREALELALIENLQREDLNLIEEAEGYRTLTEKFEMTQEQISQRVGKAR
                     ATVANSLRLLELPDSVKQKVAEGALSAGHAKVLLGLEIPHERELLAVRTLKEGLSVRA
                     LEKLVDRLRRMPRKPRAERSDLPESYLESLTDRLHQHFGTSVHVLPCRTLANGRKAKG
                     NITIEFYTNDELDRLLTLLGLSENL"
     gene            complement(1371..2099)
                     /gene="rsmI"
                     /locus_tag="PK634_13105"
     CDS             complement(1371..2099)
                     /gene="rsmI"
                     /locus_tag="PK634_13105"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOW98713.1"
                     /translation="MEPGLYIVGTPIGHLQDITLRALDTLRQADLVVAEDTRHTRVLL
                     ERHGIPARLFSCHRFNEASRTDAILDRIRAGGAVALVTDSGMPGVSDPGSRVVAACRA
                     AGLPATCVPGPSAVTAAVALCGFGGAGFTFAGFLPRKSGARRRRLEELAARHEPFVLF
                     ESPFRLLRLLEELEAVAGARPVFLGRELTKQFEECRTGTPAELRAAFAGRAVKGELVL
                     VVAPAGRHDEPDDAPPDGPAEGKC"
     gene            complement(2152..2769)
                     /locus_tag="PK634_13110"
     CDS             complement(2152..2769)
                     /locus_tag="PK634_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98714.1"
                     /translation="MKRIGSMAALVLFAALMSSAPAGAQEFSTSPQWSLGLRGGVWSV
                     PNAWLDQFLDEHPSVDGETFGITFCPLGESGSTGVFSVVYSLDYGTLSGDGRWLDDED
                     DWGDRREGEASLDLIGLSANFMWDIMPEWVVHPYLGLGLGIAYVDGRIPGEEGETDET
                     YKGPAPVLQIPVGLKAVLAETVSIHIEARILEGGLVLGGGAAVMW"
     gene            complement(2849..3775)
                     /locus_tag="PK634_13115"
     CDS             complement(2849..3775)
                     /locus_tag="PK634_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98715.1"
                     /translation="MRTRTPILCLLAAAFFRAGPAPAAEDTIADHWHDGLSHGVGHLS
                     RWIDSFFGNVPVEEERMGNRVRLTPLVEFREGDSPLFKLRLNAKLRLPQFSRRLGLVF
                     SFFDDEDAAQYGVPGQFSSQDDVRQAALRYVIEEEARARLSGDAGLRFYPEPDPYLRL
                     RWRYRLTEDPVASRVIQYLYWSPRHGWGETTRFEMESKLAPKTFARSTSAATWSEIST
                     GVDWSQSFSLWHYFSPRRAVGLDWLTEGYTDPRLSLGKTRMTFKYRRRVSRDWLFLQV
                     GPFVELPSDRDFQVTPGLTLQIETVFGDPPWL"
     gene            complement(3824..4477)
                     /locus_tag="PK634_13120"
     CDS             complement(3824..4477)
                     /locus_tag="PK634_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98716.1"
                     /translation="MKTMAFLLVFAILGGGLALAATAEEATWANEVEGGVVIQFPGTG
                     DFDLYDLGLGAEIQYRNWSFDPLGLAFAVGFTSWEANEDCHKLGDVHTEFDGSVTTIP
                     IGVSLLYYLYGTQNFSLIAEAGVRYLITDSDVDFTRIVETEEGEEQLPSSLEVDDSVL
                     GLLALEADYFVNEQFLVFGGAGYQQDLQRGEIDTPTGPLRDNEFTSLFLRLGGKFVF"
     gene            complement(4527..>5998)
                     /locus_tag="PK634_13125"
     CDS             complement(4527..>5998)
                     /locus_tag="PK634_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOW98717.1"
                     /translation="DVIYVHIDKKKKFPATALLRAFGHGHNADILRLFVDAGVRVRLD
                     PAVTARVTVDMRDEDMQAALARLLDPLGYVLTWTVVQGPLGPWPRLAEIQVFQPGRRE
                     LAEPLPGRPDQLAVAQDTNGAWVVKDEILLSFRAGGSREEAERLLAQYGGILLDGIPE
                     LGIYRVRFPPGSNVPALVAQLGQSPLVRAAEPHYAAALPPPARLNADSSAAGAAPQPG
                     LPADGAAAVAILDTGLLKDAGLASLEAGRYDALNPGRELDDRQGHGTQMALIAAGMVA
                     PAGAPASGEGVPVLAVRAFDDNGYASSFSLMSAMTYAASNGARVVNLSWGTPAESGFL
                     AAAVAWVQAQGLVVVASAGNEPTGRPLYPAACAGVVAVAALDSEGQPWESSNYGEFIS
                     VAAAGTATLPVGYRGPPGGYAGTSIASAWVARQLALYRARHPEATPAQAVRALRQAVA
                     PDGGAKDARLGYGRLDDAALSRLLPPDSRPKKLLFAPEK"
BASE COUNT          923 a         2061 c         2139 g          875 t
ORIGIN      
        1 ccacgagccg ggcagaaaat ggagaaacgc gtagatcgcg ctgaagaaga cgatgccgag
       61 cagcagttcc aggccgagcg atttgaggta atcgaggaac cagccctcgg gcgtctggtg
      121 cgacaggcca tagcgccgtt cgacggcgta gtcgccgaag tagctcaagg ggaacatcag
      181 cgccgagaag ccgaacagcg tgatcagcgt gtagagcgcg ttgaccaccg gccaccagcc
      241 gccgaaccgc gcgcgcagcc cttccgccag cagggtcgag gcgccggtca gcaggtaggc
      301 ccagaggagg cccagcgtga gcgcgagccg cagcaggaac aggcggttat gaatggcctc
      361 atactgctgc gcgcgcaggt cgtcgtccgg cggggaggcc ggggctgggt cgtcgctcat
      421 ggccggacct ctacaggttt tccgagagcc ccaggagggt caacagacgg tccagttcgt
      481 cgttggtgta gaactcgatc gtgatgttgc ccttcgcctt gcggccgttc gcaagggtcc
      541 ggcagggcag gacgtggacc gacgtgccga agtgctggtg caggcggtcg gtgagcgact
      601 ccaggtagct ctcgggaagg tcggagcgct cggcgcgggg cttgcgcggc atgcggcgca
      661 gccgatcgac cagtttttcc agggcccgga cggacagccc ctccttgagc gtgcggaccg
      721 ccagcagctc ccgctcgtgc gggatctcca ggcccagcag caccttggcg tggccggcgg
      781 acagcgcgcc ttccgcgact ttctgcttca cggaatccgg cagttccagc agccgcagcg
      841 agttggcgac cgttgcgcgg gccttgccga cgcgctgcga gatctgctcc tgcgtcatct
      901 cgaatttctc ggtcagcgtc cggtagccct cggcctcctc gatgaggttc aggtcttcgc
      961 gctgcaggtt ctcgatcagg gccagttcca gggcctcgcg gtccgagacc tccataatcc
     1021 gcgccggcac ctcgcgcaac tccgcggcct gggccgcccg gagccggcgc tcgccggcga
     1081 tgagctcgta gtggtcctcg acgcgccgca ccagcagcgg ctggagcacg ccgtgctcgc
     1141 ggatggagtg ggcgaggtcc tcgatggctt ccggcctcat ctcgtgccgc ggctgccagg
     1201 ggctcttgtg gatctggtcc acgggcagct tgacgatgcc gcgctcgccc tccgcggcgg
     1261 gcgccggctc cggcggcggg gtatccttga tcagcgcgct caggccgcgt ccgaggccta
     1321 tgtgcttggc tgccatgttg cggatagcct gtccgcgccg ccccgaaaaa tcaacacttt
     1381 ccttccgccg gaccgtcggg cggtgcgtcg tccggttcgt cgtgccgtcc ggcgggcgcc
     1441 acgacgagga ccagctcgcc cttcacggcg cggcctgcga aggcggcccg gagttccgcc
     1501 ggcgtgcccg tgcggcattc ctcgaactgc ttggtcagct cacgcccgag gaaaacgggc
     1561 cgcgcgccgg cgacggcctc cagttcctcc agcaggcgga ggagccggaa cggcgattcg
     1621 aagaggacga acggctcgtg gcgggccgcc agttcctcga gccggcgccg ccgggcgccg
     1681 cttttccggg gcaggaaccc ggcgaaggtg aagcccgcgc cgccgaagcc gcagagggcc
     1741 acggccgccg tcacggcgga cgggcccggc acgcaggtgg ccggcaggcc ggccgcgcgg
     1801 caggcggcga ccacgcggct gccggggtcg gatacgcccg gcatcccgga gtcggtgacc
     1861 agcgccaccg cgccgccggc ccggatgcgg tcgagaatcg catccgtccg cgaggcctcg
     1921 ttgaagcggt gacagctgaa gaggcgcgcc gggatgccgt ggcgctccag caggacgcgc
     1981 gtgtgccgcg tgtcctcggc gacgaccagg tccgcctgcc gaagggtgtc cagcgcgcgc
     2041 agcgtgatgt cctgcaggtg gccgatcggc gtgccgacga tgtacagccc gggctccatg
     2101 gccgtcagtg taggacggcc cggcaggccg gccaatcgaa aacggggccg cctaccacat
     2161 cacggccgcg ccgccgccca ggacgaggcc gccctccagg atccgggcct cgatatgaat
     2221 ggagacggtt tccgccagga ccgccttgag gccgacaggg atctggagga ccggcgccgg
     2281 gcccttgtag gtctcgtcgg tttcaccctc ttcccccggg atccggccgt cgacgtacgc
     2341 gatgccgagg cccaggccga ggtagggatg gacgacccac tcgggcatga tgtcccacat
     2401 gaagttggcg gaaaggccga tgaggtcgag cgacgcctcg ccctcgcgcc ggtcccccca
     2461 gtcgtcctcg tcgtccagcc agcggccgtc gccggacagg gtgccgtagt cgaggctgta
     2521 gacgacgctg aacacgcccg tcgagccgga ttcgccgagg ggacagaacg tgatcccgaa
     2581 ggtctcgccg tccacggagg gatgctcgtc gaggaactgg tcgagccagg cattcggcac
     2641 gctccatacg ccgccgcgca ggccgaggct ccactggggt gaagtggaaa actcctgggc
     2701 ccccgcgggg gccgacgaca tcagggcggc gaacaggacc aacgcggcca tgcttccaat
     2761 tctcttcatc tgaattcctt tccgagtttc gtgaaacctg aagaaaagcg gcgcggatgg
     2821 caagcgcgat ctgggcgcgc cgcttttttt acagccacgg cgggtcgccg aacacggttt
     2881 cgatctggag cgtcaggccg ggggtaacct ggaagtcgcg atcgctcggc aactccacga
     2941 aggggcccac ctggaggaag agccagtccc gggacacgcg acggcggtat ttgaaggtca
     3001 tgcgcgtttt gcccagcgac aggcgcgggt cggtgtagcc ctccgtgagc cagtcgagcc
     3061 cgacggcgcg ccgcggggag aagtagtgcc aaagggaaaa cgactgcgac cagtccacgc
     3121 ccgtgctgat ctcggaccac gtggcggcgg aggtggagcg ggcgaaggtc ttgggcgcga
     3181 gcttgctctc catctcgaac cgcgtggttt ccccccagcc gtgccgcggg ctccagtaca
     3241 ggtactggat gacgcggctg gccaccggat cctcggtcag ccggtagcgc caccggagtc
     3301 gcaggtaggg gtccggctcg ggatagaacc gcagcccggc gtccccgctc aaccgggcgc
     3361 gcgcctcctc ctcgatcacg tagcgcaacg cggcctggcg gacgtcgtcc tggctggaaa
     3421 actggccggg cacgccgtac tgcgccgcgt cctcgtcgtc gaagaaggag aacacgagcc
     3481 cgagccggcg gctgaactgg ggcagccgca acttggcatt cagccttaat ttgaacaggg
     3541 gcgaatcgcc ctcccggaac tccacgaggg gcgtcaggcg gacgcggttg cccatgcgct
     3601 cctcctcgac cgggacgttg ccgaagaagg aatcgatcca gcggctcaag tgcccgacgc
     3661 cgtgcgagag cccgtcgtgc caatggtcgg cgatcgtgtc ctccgcggcg ggggccggcc
     3721 cggcgcggaa gaaggcggcg gccagcaggc acaggatggg cgtccgcgtc ctcatgagac
     3781 atcccgcgcg ggaagacctc tcccccgatg gcccggcggc gggttaaaag acgaacttgc
     3841 cgcccagccg caggaacagg ctcgtgaact cgttgtcgcg cagggggccg gtcggcgtgt
     3901 cgatctcgcc gcgctggagg tcctgctggt accccgcgcc gccgaacacg aggaactgct
     3961 cgttcacgaa gtagtccgcc tcgagggcca gcagcccgag cacgctgtcg tcgacctcca
     4021 ggctggaggg cagctgctcc tcgccctcct cggtctccac gatccgcgtg aaatcgacat
     4081 cgctgtccgt gatgaggtag cgcacgccgg cctccgcgat gaggctgaag ttctgggtcc
     4141 cgtagaggta gtagagcagc gagacgccga tggggatcgt ggtgaccgac ccgtcgaatt
     4201 ccgtgtgcac gtcgcccagc ttgtggcagt cctcgttggc ctcccacgag gtgaagccca
     4261 cggcgaaggc gagcccgagg ggatcgaagg accagttgcg gtactggatt tccgcgccga
     4321 gcccgaggtc gtacaggtcg aaatcgcccg tgccggggaa ctggatcacc acgccaccct
     4381 cgacctcgtt ggcccacgtt gcttcttccg ccgtggccgc cagcgccagg ccgccaccga
     4441 ggatcgcgaa caccaggagg aatgccatcg ttttcatgtg ctgcctttcc tttcgcttcc
     4501 cgcgcgtatc gttcggccca ctctattcat ttttcggggg caaacaacag ttttttcggg
     4561 cggctgtccg ggggcaggag gcgcgaaagc gccgcgtcgt cgagccggcc gtaccccagg
     4621 cgcgcgtcct tcgccccgcc gtccggggcc acggcctgcc gcagcgcccg gaccgcctgc
     4681 gccggcgtcg cctcgggatg gcgcgcccgg tagagggcga gctggcgggc gacccaggcg
     4741 ctcgcgatgg acgtgcccgc gtaaccgccg ggcgggccgc ggtagccgac tggcagcgtg
     4801 gccgtgccgg cggcggccac cgaaatgaac tcgccgtagt tggacgactc ccacggctgg
     4861 ccctccgagt ccagcgcggc gacggccacc acgccggcgc aggcggcggg atagaggggc
     4921 cggccggtcg gctcgttccc ggccgaggcg accacgacca gtccctgcgc ctggacccag
     4981 gccacggcgg cggccaggaa cccgctctcc gccggcgttc cccagctcaa gttcaccacg
     5041 cgcgcgccgt tcgacgccgc gtacgtcatg gcgctcatca gcgagaagga ggaggcgtac
     5101 ccgttgtcgt cgaaggcccg gacggccagc accggaacgc cttcgccgga ggccggcgcg
     5161 ccggcgggcg cgaccatgcc cgcggcgatc agggccatct gggtgccgtg tccctgccgg
     5221 tcgtccagct cccggcccgg attcagcgcg tcgtagcgcc ccgcctcgag cgaggccagt
     5281 ccggcgtcct tcaggagccc ggtgtccagg atcgccacgg ccgccgcgcc gtcggccggg
     5341 aggcccggtt gcggcgccgc gccggccgcg gaagaatcgg cgttgagccg cgcgggcggg
     5401 ggcagcgcgg cggcgtagtg cggttccgcg gcccgcacga gcgggctttg gcctagctgc
     5461 gccacgaggg cgggcacgtt ggaacccggc gggaagcgca cgcggtagat gcccagttcc
     5521 gggatcccgt cgagcaggat gccgccatat tgcgccagca gacgctccgc ctcctcccgg
     5581 gagccgcccg cccggaacga gagcaggatt tcatccttca cgacccacgc gccgttcgtg
     5641 tcctgcgcga cggccaactg gtcggggcgg ccggggaggg gctccgccag ctcccgccgg
     5701 cccggttgaa acacctggat ttccgcgagc cggggccagg ggccgagcgg accctggacc
     5761 accgtccacg tcagcacgta gccgagcggg tccaggagcc gggcgagcgc ggcctgcatg
     5821 tcctcgtcgc gcatgtccac ggtgacgcgc gccgtgacgg cgggatccag ccgcacgcgc
     5881 acgccggcgt cgacgaacag ccggaggatg tcggcattgt ggccgtggcc gaaggcgcgc
     5941 agcagcgcgg tggccgggaa cttcttcttc ttgtcgatgt ggacgtagat cacgtcgt
//
LOCUS       DAOOTK010000161         5981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8234_length_5981_cov_5.775059, whole genome shotgun sequence.
ACCESSION   DAOOTK010000161 DAOOTK010000000
VERSION     DAOOTK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8234_length_5981_cov_5.775059"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK634_13130"
     CDS             complement(<1..839)
                     /locus_tag="PK634_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOW98718.1"
                     /translation="MSKRTLRGLTILMLLQAVAMTVVWAQEPAAPAPAPAAAPDVAAA
                     PAPAAKKMTLWNLIVVGGWAMWPLGACSLAMVGLAVLNFRQINRKRMLPEAIIAQLRV
                     AAKEQNLETMVNLAASTSTLFTNGLMAGLRKFNTEDPMGCKPNVESAIAEAVGREESQ
                     ASFWINFLSLVTAMSPMWGLIGTVSGMIGAFQKIGSGGMGKPELLAANIGEALITTAA
                     GLIIAIPSMFFYFLFRNLLNRIIQQAELTYSLLLDDLTGSTFIVEEAPPTYEQLPEAP
                     PLSV"
     gene            complement(878..1861)
                     /locus_tag="PK634_13135"
     CDS             complement(878..1861)
                     /locus_tag="PK634_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98719.1"
                     /translation="MRARHAIAGTAILLGAALGAFAQTPAEPGVGPALVLTKDGQFPA
                     RLLRRDQEMLWVSKSTEDGSAFEAGIPLADIQKIRMPSPRVFAAADVAANDEQFRAVH
                     DALDRLIQSLKPFRGLPGVPMDEAILRKGQLYARQGLWREAIRQYEDILKQPYECEQK
                     TAARLRAGIASELAGDAKAALTYFENVVLPEDDEELLSTALFSRANALAADGRPQDAL
                     LDYLRLVVFHPYMQNNELRGLEAAVGCYAQLKDWESLHKTVAVLQQDYPGTRESKHAA
                     EVEAEHRAEMEAAGTVLDEGATVEPEGEPSAAAASTSAPPVRADTETIQAD"
     gene            complement(1884..4136)
                     /locus_tag="PK634_13140"
     CDS             complement(1884..4136)
                     /locus_tag="PK634_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98720.1"
                     /translation="MSRARVMLSLLAIGILALSPDARAARWQKLSNCQLAAGRPHDGD
                     TLSVTHAGQDYVFRLYFVDAAETNRENAAFLVNQATYWDIPDMHVVRVGREASEFVEK
                     TLQPGFAVLTQWEKAGGTAESPVHFAMVQVGEDDLAELLVEQGLARIHGASTPPPDGT
                     SLEDFTHRLRQAESRAKHRKAGAWGIAEPPEPGAALSVTPAADQAPPPPVQWPNVPIV
                     AFLRAEAYVNTERFEEAEVAMRALLRRFPEHVQKPRIEFYLGLSLAMQERFEEAVTIL
                     RSWLSKYPTHLMATEVQYWLPIALFYGGQYGEARPLFEEYAAKNGMSIYAPEALYRAA
                     LCQYAVEDFEGAAESLEAWVQANPDHYFHWEALVTLGDALAAIGELDRAKGAYLRVEK
                     AGGAFQYMALTQCAKVFKALGTPDDFRDMAEAFAAFIRENPDNDNIIDAAYQAGWALR
                     QNRRLDEARRLYWSMIELHGNTVGWDGFDLMLQDLAGMYGDEPGGFPAVLAEKKQKAL
                     DDKRMTLAARLNMADVQIRPPEEQGRAALILASSFKPEQLGLETLAWLGDQLVQQGKT
                     NEASICFEQLLKIGPESMNAALAHTRLAEQLVKAGRAEEALVHADAALANPAEPTLVM
                     EATFARAKSLQAAERYSEAIADYATVLANRSSPRALKPESLLATAACLEAQDRPRQAI
                     PYYQRIYVLYGAYTGAVAQAYLKSGAAFEEIKDFESAAKTYREMLELDSMSGTKEAVE
                     ARQRLERLGV"
     gene            complement(4138..5280)
                     /locus_tag="PK634_13145"
     CDS             complement(4138..5280)
                     /locus_tag="PK634_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98721.1"
                     /translation="MAAAACVFLASTAGAQAQAEGPEELYRRGVGEFAAGNYAEAATA
                     FKELITNFGNEPSLAAQMEGAFYAYGCCLYNQGQHADAIEAFKSYAAKYPEARYRDEA
                     LYRIAVAHQILEAWEAAVAAYKDLLAQYPRSAYAEDTAYQLGMVFMLQDKLPEGVQAF
                     ADFMTAFPASEYWGQAGAFIARALFDQDKVVEAIAMLQKLEQRPRSWSVITYCNFLAF
                     EIGDAAFDETEYELALKAYRRVKPRAILLRRQGETVETLRAELERFRRAKAAPEKVQA
                     RFMQERRLQGSLAQAEELFKKLESLPDYDANLYHRIGRCFFNLDRFWEARVAFMRVVE
                     IASEDKIREAGHFDLVLCLSRLRRFDELIDEADRYLDRYDPEWTRN"
     gene            complement(5339..>5981)
                     /locus_tag="PK634_13150"
     CDS             complement(5339..>5981)
                     /locus_tag="PK634_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOW98722.1"
                     /translation="WPRVVYIHAHAAFGWILAQVVGRGDARFRRAVFLSAIAPDFDAM
                     AYLLGLSISLDQHHVWTHNLLFSWALSLAAMLYCPNMKMRILLLTQLAFYAHYFGDYF
                     FTTWPLCFLYPFSPRPFLSAHAFHLWHPVNQWLGAVLAAVALMLCFVCRRTPLELVSP
                     ALDRAVVRGLGARLASLRSFMNRGRRRPDPPPQPGSGERTNRRRSIERKESKG"
BASE COUNT          974 a         2014 c         1961 g         1032 t
ORIGIN      
        1 acggacaggg ggggcgcctc cggcagctgc tcgtacgtcg gcggcgcctc ctcgacgatg
       61 aaggtggaac cggtcaggtc gtccagcagc agggaatagg tcaactccgc ctgctggatg
      121 atccggttca gcaggttgcg gaagaggaag tagaaaaaca tggacggaat ggcgatgatc
      181 agcccggctg ccgtggtgat cagggcctcg ccgatgttgg cggcgagcag ttccggcttg
      241 cccatgccgc cggacccgat cttctggaac gcgccgatca tgccgcttac tgtgccgatc
      301 agcccccaca tgggcgacat ggccgtcaca agcgacagga agttgatcca gaaactcgcc
      361 tgcgactcct cgcggccgac cgcctccgca atggcggatt ccacgttggg tttgcaaccc
      421 ataggatctt ccgtgttgaa cttgcgcaaa ccggccatca ggccgttggt gaacagggtg
      481 gatgtgctgg ccgccaggtt caccatggtc tcgaggttct gttccttggc ggcgacccgc
      541 aactgggcga tgatggcctc cggcagcatc cgcttgcggt tgatctggcg gaagttaagc
      601 acggccagcc cgaccatggc cagggaacaa gccccgagcg gccacatggc ccagccgccc
      661 accacgatca ggttccacag cgtcatcttc ttggctgccg gagccggggc agcggccaca
      721 tccggggcgg cagccggggc cggcgcgggc gcggcgggct cctgggccca gaccacggtc
      781 atggccaccg cctgaagcag catcaatatt gtcaatccgc gcagcgttct cttgctcatg
      841 ggatatccgc tccttttcct ggttcgaaaa tcttccttca atccgcctgg atcgtctccg
      901 tatccgcccg gacgggcggc gcgctggtgg atgcggcggc agcggagggc tctccctccg
      961 gctccacggt cgccccctcg tccagcaccg tcccggccgc ttccatctcc gcgcggtgct
     1021 ccgcctccac ctccgcggca tgcttgctct cgcgggttcc cgggtagtcc tgctgcagga
     1081 ctgccaccgt cttgtgcagg gactcccaat ccttgagctg cgcgtagcag ccgacggccg
     1141 cctcgagccc gcgcagttca ttgttctgca tgtacgggtg gaagacgacg aggcgcaggt
     1201 aatccagcaa ggcatcctgc ggccggccgt cggcggccag cgcattggcg cgggagaaca
     1261 acgccgtgga aagcaactcc tcgtcgtcct cgggcagcac gacattctcg aagtacgtca
     1321 gggccgcctt ggcgtcgccc gcgagttcgc tggcaatgcc ggcgcgcagc cgcgcggcgg
     1381 tcttctgctc gcactcgtag ggctgcttga gaatgtcctc gtactgccgg atcgcctcgc
     1441 gccacaggcc ctgccgcgcg tagagctggc ccttgcgcag gatcgcctcg tccatcggta
     1501 cgccgggcag gccgcggaac ggcttgagcg actggatcag ccggtcgagc gcatcatgga
     1561 cggcgcggaa ttgctcgtcg ttggccgcta cgtccgcggc ggcgaatacg cggggcgagg
     1621 gcatccggat cttctggatg tcggccagcg ggatgccggc ctcgaaggcg ctgccgtcct
     1681 ccgtgctctt gctgacccag agcatctcct ggtcccgccg cagcaggcgc gccgggaact
     1741 ggccgtcctt ggtcagcacc agggccggac ccacgcccgg ctcggccggg gtctgcgcga
     1801 aggcgcccag cgccgcgccc agcaggatcg cggtccccgc gatcgcatgt cttgctctca
     1861 tgccgttttt catcgccgcc gtcctagacg cccaaccgct cgagccgttg ccgcgcctcg
     1921 acggcttcct tcgtgccgga catcgaatcc agctccagca tttctcgata ggtcttcgcc
     1981 gccgactcga aatccttgat ctcctcgaag gccgcgccgc tcttcaggta ggcctgcgcg
     2041 acggcgcccg tgtacgctcc gtacagcacg taaatgcgct ggtaatacgg gatcgcctgc
     2101 cgcggccggt cctgcgcctc gagacacgcc gcggtggcca gcagggactc gggcttgagc
     2161 gcccggggac tcgagcggtt cgccagcacg gtggcgtagt ccgcgatcgc ctcgctgtac
     2221 cgctcggcgg cctggaggct tttcgcgcgc gcgaacgtcg cctccatgac cagcgtcggc
     2281 tcggcagggt tggccagggc ggcatccgca tggaccagcg cctcctcggc gcggcccgcc
     2341 ttgaccaatt gctccgccaa cctcgtatgg gccagggcgg cgttcatcga ttccggcccg
     2401 atcttcaaca gttgttcgaa acagatcgag gcctcgttcg tcttcccctg ctggaccagc
     2461 tgatcgccga gccacgccag ggtctcgagg cccagttgct ccggcttgaa actcgaggcc
     2521 aggatgaggg ccgcgcggcc ctgctcctcg ggcgggcgga tctggacgtc tgccatgttc
     2581 aggcgggcgg cgagggtcat ccgcttgtcg tcgagggcct tctgcttctt ctccgccagg
     2641 acggcaggga agccgccggg ctcgtcgccg tacatccccg ccaggtcctg gagcatcagg
     2701 tcgaacccgt cccagcccac ggtgttgccg tgcagttcga tcatgctcca gtagagccgc
     2761 cgcgcctcgt ccaggcggcg gttctggcgg agggcccagc cggcctggta cgccgcgtcg
     2821 atgatgttgt cgttgtcagg attctcgcgg ataaacgccg cgaaggcctc ggccatgtcc
     2881 ctgaaatcgt ccggcgtgcc gagcgccttg aagaccttcg cgcattgcgt cagggccatg
     2941 tactggaagg cgccgccggc cttctcgacg cgcaggtagg cgcccttggc gcggtccagt
     3001 tcgccgatcg cggccagggc gtcgcccagc gtgaccagcg cctcccagtg gaaatagtgg
     3061 tccgggttgg cctgcaccca tgcctccagc gactcggcgg ccccctcgaa atcctcgacg
     3121 gcatactggc acagcgcggc gcggtagagc gcctcgggcg cgtatatgct catgccgttc
     3181 ttcgcggcgt actcctcgaa gagcggccgc gcctcgccgt actggccccc gtagaaaagg
     3241 gcgatcggca gccagtattg cacctcggtg gccatgagat gggtgggata cttggacagc
     3301 cagctgcgga ggatggtcac cgcctcctcg aaccgctcct gcatggcgag cgaaagaccc
     3361 aggtagaact cgatgcgcgg cttctggacg tgctccggga agcggcgcag cagcgcgcgc
     3421 atcgccacct cggcctcctc gaaccgctcc gtgttgacgt aggcctccgc gcgcaggaac
     3481 gcgacgatgg gcacgttggg ccactggacc ggcgggggcg gcgcctggtc cgccgcgggc
     3541 gtgaccgaga gggccgcgcc gggctcgggc ggctccgcga tcccccaggc gcccgccttc
     3601 cgatgcttcg ccctggactc ggcctggcgg aggcggtggg tgaaatcctc gaggctggtt
     3661 ccatcgggcg gcggcgtgct ggccccgtga atccgggcga gaccctgctc caccagtagt
     3721 tccgccaggt cgtcctcccc gacctgcacc atcgcgaagt gcacgggcga ttccgcggtg
     3781 ccgccggcct tctcccattg cgtcagcacc gcgaatccgg gctgcagcgt cttctcgacg
     3841 aattcgctgg cctcgcgccc cacccggacc acgtgcatgt ccgggatgtc ccagtaggtc
     3901 gcctggttca ccaggaaagc ggcgttctcg cggttggttt cggcggcgtc cacgaaatag
     3961 agccggaaaa catagtcctg gcccgcgtgg gtcacgctca acgtgtcgcc gtcgtgcggc
     4021 cggccggcgg ccaactggca gttgctcaac ttctgccacc gcgcggcccg ggcatccggc
     4081 gaaagcgcca ggatcccgat cgccagcagg gacagcatga cccgcgcgcg gctcatttca
     4141 atttcgtgtc cattccgggt cgtagcgatc caggtaacgg tcggcctcgt cgatcagctc
     4201 atcaaagcgt cgcaagcgcg aaaggcagag caccaggtcg aagtggcctg cctcccggat
     4261 cttgtcctcc gaggcgatct cgaccacccg catgaacgcc acgcgggctt cccagaagcg
     4321 gtccaggttg aagaagcagc gcccgatccg gtggtagagg ttggcatcgt aatccggcag
     4381 ggactccagc ttcttgaaca attcctccgc ctgggccagc gatccctgca ggcggcgctc
     4441 ctgcatgaag cgggcctgga ccttttccgg cgcagccttg gcccgccgga agcgctccag
     4501 ttcggcccgg agggtctcaa cggtctcccc ctgcctccgc agcaggatag cgcgcggctt
     4561 cacccggcga taggccttga gggccagttc gtattctgtc tcgtcgaagg cggcgtcgcc
     4621 gatttcgaag gccaggaaat tgcagtaggt gatcacgctc cagctccggg gccgttgctc
     4681 gagtttctga agcatggcaa tggcctcgac gaccttgtcc tggtcgaaaa gcgcccgggc
     4741 gatgaacgcc cccgcctggc cccagtactc cgacgcgggg aaagcggtca tgaaatccgc
     4801 gaaggcctgc acgccctccg gcagcttgtc ctgcaacatg aagaccatgc cgagttggta
     4861 ggcggtgtcc tccgcatacg cggaacgcgg gtactgggcc agcaggtcct tgtaggcggc
     4921 gacggcggct tcccacgcct cgaggatctg gtgcgcgacg gcgatgcggt agagcgcctc
     4981 atcgcggtag cgagcctcgg ggtacttcgc ggcgtatgac ttgaaggcct cgatcgcgtc
     5041 cgcgtgctgg ccctggttat acaggcagca gccgtaggcg tagaaggcgc cttccatctg
     5101 cgcggccaac gagggctcgt tgccgaagtt ggtgatcagt tccttgaacg cggtggcggc
     5161 ttccgcgtaa ttgccggcgg cgaactcacc gacgccgcgc cggtagagct cctcaggacc
     5221 ctcggcctgg gcttgggcgc ctgccgtgga ggcaaggaat acgcaggctg ccgcagccac
     5281 cgatctccat actggccacg cgttcgtcat tcggagggtc tagacccaga ccttatcatt
     5341 acccttttga ttccttccgt tcaatacttc ttctccgatt tgtgcgctcc cccgatccgg
     5401 gttgaggggg ggggtcgggt cgccgcctcc cgcgattcat gaaagaccgc aaggacgcca
     5461 ggcgggctcc gagcccgcgc accaccgccc ggtccagcgc cggcgaaacc aattccagcg
     5521 gggtgcgtcg gcaaacaaag cagagcatca gcgccacggc ggccagtacg gctcccagcc
     5581 actggttgac cggatgccac aaatggaagg cgtgcgcgct tagaaacggg cggggcgaaa
     5641 agggatagag gaaacacagc ggccacgtgg tgaagaagta gtcgccgaag tagtgggcat
     5701 agaacgctag ctgggtaagc agaaggatgc gcattttcat gttcgggcaa tacagcatgg
     5761 ccgccagtga tagcgcccag gagaagagca ggttatgcgt ccaaacatga tgctgatcga
     5821 gcgagatgga aagaccgagc agataggcca tcgcatcgaa gtccggcgcg atagccgaga
     5881 gaaagacggc gcggcgaaag cgcgcatcgc cccggcccac cacctgcgcc aggatccagc
     5941 cgaaagcagc atgggcatga atgtacacga cgcgcggcca t
//
LOCUS       DAOOTK010000162         5957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8281_length_5957_cov_6.222128, whole genome shotgun sequence.
ACCESSION   DAOOTK010000162 DAOOTK010000000
VERSION     DAOOTK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8281_length_5957_cov_6.222128"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK634_13155"
     CDS             complement(<1..1074)
                     /locus_tag="PK634_13155"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOW98723.1"
                     /translation="MRNVIRWLAIAGFCAAVLSPAFAARLTLQHDLHGYAGASDTWLD
                     ESATHDNYGGDSKLTVKYNSGPKSDGALVRFALPAVPCDAVTSAVLGLFYLEAGSMSS
                     GNAMGLKPYRVNAGRNWFENTRNGQSGEGASWRYFGRDEVETNQWTAEDGGWQDKTDD
                     GNSSNLIKKAGGEVPGAIEPTNWVPFDVRRSVTNWLGGAPNNGLILFSCSFEGSGYIV
                     NGLFAARETNHAAIRPKLSLQYQGAQIVWTGRVSGAWDTAATNWSVGGWPGQYDNGDH
                     VRFTDAAVQTNIAVAAGLAPASVTVSNAVRPFAFSGGPLGGTGLVLKTGAGMLTLAAS
                     NSYSGPTRVEAGILVAASNGALGS"
     gene            complement(1139..2170)
                     /gene="pheS"
                     /locus_tag="PK634_13160"
     CDS             complement(1139..2170)
                     /gene="pheS"
                     /locus_tag="PK634_13160"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOW98724.1"
                     /translation="MTIEEIRKIRDEALAEADAAADAASVEAARIKYLGRKGLLPKIM
                     ESLKDLAPADRPEAGKLSNELKNGLAARLEARKQALGQAAPARAAGLDWSLPGRWRGL
                     GSRHPVSLVIARAADIFRSLGFTVADGPDIETEYYNFDALNTPGDHPARDSQDTFYFS
                     SGQPATAGKLLRTQTSPVQIRVMEKQKPPVRIIAPGRCYRRDTTDATHSANFHQIEGL
                     YVDRNVSLADLKGDLTHFARQMMGADVRIRFRPHFFPFTEPSVEYDFSCHVCGGKGCR
                     VCKDSGWIEISGAGMVNPKVFRAVGYDPDEVTGYAFGMGVERIAMILYGIPDIRMLYD
                     NDARFLAQF"
     gene            2339..3796
                     /locus_tag="PK634_13165"
     CDS             2339..3796
                     /locus_tag="PK634_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOW98725.1"
                     /translation="MKRCLRLLLCLVFAASAAARDVRDSIVKIYTVHNSPDYFNPWSM
                     RGPFSTTGSGCVVPGKRILTNAHVVGDQTFIQVRRNGEAKRYVARVAAVAHDADLALL
                     AVDDPSFFEGVEPLEFGELPQTQQEVLVYGFPMGGDTLSITKGVVSRIEHQNYTHSSC
                     NLLSVQIDAAINPGNSGGPAIVGGRIVGVAMQAMRQADNIGYLVPVPVIQHLLKDLED
                     GQRGGIPSLGLILEDMENPGLRKKHQLPDDRTGVLILRIAHGSPAEGLLKEGDVLLSV
                     DGHAIANDGTVEFRPRERTSVSYWIQQHQVGETANLDILRDGEALPVQVRLTSRMEQD
                     WIIPFEEYDILPTYYIYGGIVFSPLTVNLLQSWGGNWYEKAPRDLVARLGFNLVTPEQ
                     DEVVLALRVLAADVNEGYHNVSSWTVDKVDGEKVRNLKHLIELIEKGSGPFVVLENEW
                     GQKLVLDREQSEQTGPQILGTYRIPKDRSPDLAAP"
     gene            complement(3827..4183)
                     /gene="rplT"
                     /locus_tag="PK634_13170"
     CDS             complement(3827..4183)
                     /gene="rplT"
                     /locus_tag="PK634_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOW98726.1"
                     /translation="MSRATNAPASRRRRKRRLGLAKGFYGGRSKRFRMATEAVDRAMV
                     QATKHRKLRKREFRNLWVVRLNAACRELGLSYSRFMEGVTKAGIRLNRKMLSEIAIHD
                     PDGFASIVDLAKKKLA"
     gene            complement(4195..4392)
                     /gene="rpmI"
                     /locus_tag="PK634_13175"
     CDS             complement(4195..4392)
                     /gene="rpmI"
                     /locus_tag="PK634_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOW98727.1"
                     /translation="MAGKYKTRKAVSKRFHKTAGGVIKHACGSRSHLLGHKSRKRKRQ
                     LRAGSTVSSADYGRISRGLPL"
     gene            complement(4497..5054)
                     /gene="infC"
                     /locus_tag="PK634_13180"
     CDS             complement(4497..5054)
                     /gene="infC"
                     /locus_tag="PK634_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOW98728.1"
                     /translation="MNNRIRVPEVRCIGPDGQQIGVITTRDALERASREGMDLVEISP
                     NAQPPVCRIMDYGKFKYEQEKKDKQARRHQSATRVKEVQFHPNVGDHDYQTKVRHTRE
                     FLQEGHRVKIGLFFRGRERAHEEIGFAVMNRVLKDCQDIGTPEQMPRFIGRAIFMLLC
                     PKPGAKPKAAEAKPAPAALETAPAQ"
     gene            complement(5138..>5957)
                     /locus_tag="PK634_13185"
     CDS             complement(5138..>5957)
                     /locus_tag="PK634_13185"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOW98729.1"
                     /translation="VLAGLFRVRGFTQDDAHIYCTPEQVEDEVLRAMHFAIRMWKTFG
                     FSSMSAYLSTRPDKAVGEPERWEQATRSLEQALKEAGIPYEVDPGGGAFYGPKIDMRV
                     KDAIGREWQMSTIQFDFNLPERFDMAFTGADGKDHRPYMVHRALFGSIERFFGILTEH
                     YAGAFPFWLAPEQARVLPLTDKQAEYARSVAAALRDAGFRVGLDERPEKLGARIRDAQ
                     LMKIPYMLVAGGREAEAKTVAVRSRTEGDRGAVDLPALITMWKAEEDRRGLAPG"
BASE COUNT          907 a         2055 c         1974 g         1021 t
ORIGIN      
        1 cgagcccagg gccccgttgc tggccgcgac cagtatcccg gcctcgacgc gggtcggacc
       61 gctgtacgaa ttgctcgcgg ccagcgtcag catccccgcc ccggttttga gcaccaggcc
      121 ggtcccgccg agcgggccgc cggagaacgc gaacggccgg accgcgttgc tgaccgtcac
      181 cgaggccggc gcgaggccgg cggccaccgc gatgttggtc tggaccgccg cgtcggtgaa
      241 gcggacgtga tccccattgt cgtactgccc gggccagccg ccaaccgacc agttcgtcgc
      301 cgccgtgtcc cacgcgccgc tgacccggcc cgtccacacg atctgcgcgc cctggtactg
      361 gaggctcaat ttcggccgga tcgccgcgtg gtttgtttcg cgggcggcaa agaggccgtt
      421 cacgatgtac ccgctcccct cgaagctgca cgagaacaaa atgagcccgt tgttcggcgc
      481 gccacccagc cagttggtca cgctgcgccg aacgtcgaac ggcacccagt tggtcggctc
      541 gatggcgccc ggcacctcgc cccccgcctt cttgatcagg ttgctgctgt tgccgtcatc
      601 ggttttatcc tgccacccgc cgtcctcggc cgtccactgg ttcgtctcca cttcgtcccg
      661 gccgaagtag cgccaactgg ccccctcgcc cgactggccg tttcgcgtgt tctcaaacca
      721 gttccggccc gcgttcacgc gatagggttt caagcccatg gcgttccccg agctcatact
      781 gcccgcttcg aggtagaaca ggcccaggac ggcggaggtc accgcgtcgc acggcacggc
      841 cggcagggca aaccggacca gggcgccatc cgacttgggc ccgctgttgt atttcaccgt
      901 cagcttggag tcgccgccgt aattgtcgtg cgtcgcgctc tcgtccagcc acgtgtcgct
      961 cgcgccggca tagccgtgga ggtcgtgctg cagggtcagc cgggcggcga aggcggggga
     1021 cagaacagcc gcgcagaatc cggcgatggc aagccagcgg atgacgtttc gcatgcgccc
     1081 ggcaccctcc cctctttttt tatccccccg ccgggaagac gcgtcaagac aaagcgaatc
     1141 agaactgcgc caggaaccgg gcgtcgttgt cgtagagcat ccggatgtcc gggatgccgt
     1201 agaggatcat ggcgatgcgc tcgacgccca tgccgaaggc gtagccggtg acctcgtccg
     1261 ggtcgtagcc gaccgcgcgg aagaccttgg gattgaccat gcccgcgccg gagatctcga
     1321 tccagccgct gtccttgcag acgcggcagc ccttgccgcc gcagacgtgg cacgagaaat
     1381 cgtactcgac gctcggctcg gtgaacggga agaaatgcgg ccggaaccgg atgcgcacgt
     1441 cggcgcccat catctggcgc gcgaagtgcg tcaggtcgcc cttgaggtcg gccagcgaga
     1501 cattccggtc cacgtacagc ccctcgatct ggtggaagtt cgcgctgtgc gtcgcgtcgg
     1561 tcgtgtcgcg ccggtagcag cggcccggcg cgatgatccg gacgggcggc ttctgcttct
     1621 ccatcacccg gatctgcacg ggcgaggtct gcgtgcgcag cagcttgccc gccgtggcgg
     1681 gctggccgga ggaaaaatag aacgtgtcct gcgaatcccg cgccgggtgg tcgcccggcg
     1741 tgttgagcgc gtcgaagttg tagtactcgg tctcgatgtc cggcccgtcg gcgacggtga
     1801 agccgaggga gcggaagatg tccgccgcgc gcgcgatgac gaggctgacc ggatgccgcg
     1861 agcccagccc gcgccaccgg ccgggcagcg accagtccag cccggccgcg cgcgcggggg
     1921 ccgcctgccc cagcgcctgc ttgcgcgcct cgagccgggc ggccaggccg ttcttcaact
     1981 cgttggagag cttgcccgct tccggccggt cggccggggc caggtccttg aggctctcca
     2041 tgatcttggg aaggagccct ttccgcccga gatacttgat gcgcgccgcc tcgacggagg
     2101 ccgcgtccgc ggcggcgtcc gcctcggcca gcgcctcgtc ccggatcttc ctgatttcct
     2161 cgatcgtcat gcggctcgct ccgtttttcc aatgtttgga aaattcggac ccattttttc
     2221 caatgtttgg aaaaattgca gtattttttt ccaaacattg gaaaaaaccg ccggccgcgc
     2281 cataatgaac gtcgacgcgc gatccgcgga ctaaaccaga aacccgataa ggacccccat
     2341 gaagcgttgc ctccgcctcc tgttatgcct cgtgttcgcc gcctccgccg ccgcccgcga
     2401 cgtgcgcgac tcgatcgtga agatctacac ggtccacaac tcgccggact acttcaaccc
     2461 gtggagcatg cgcgggccgt tctccaccac cggctccggc tgcgtggtgc cgggcaagcg
     2521 gatcctgacc aacgcgcacg tcgtcggcga ccagacgttc atccaggtcc ggcgcaacgg
     2581 cgaggccaag cgctacgtcg cccgcgtcgc ggccgtcgcc cacgacgcgg acctggcgct
     2641 gctggccgtg gacgacccct cgtttttcga gggggtcgag ccgctggagt tcggcgagct
     2701 gccgcagacg cagcaggagg tgctggtcta tggattcccg atgggcggcg acacgttgag
     2761 catcacgaag ggcgtcgtct cgcgcatcga gcaccagaac tacacgcaca gctcctgcaa
     2821 cctgctgtcc gtgcagatcg acgccgcgat caacccgggc aacagcggcg ggcccgcgat
     2881 cgtcggcggc cggatcgtgg gcgtggccat gcaggcgatg cggcaggccg acaacatcgg
     2941 gtacctcgtg cccgtgccgg tcatccagca tctcctcaag gacctggagg acgggcagcg
     3001 cggcgggatc cccagcctgg ggctcatcct ggaggacatg gagaatcccg gcctgcgcaa
     3061 gaagcaccag ctgccggacg accgcacggg cgtgctgatc ctgcgcatcg cgcacggctc
     3121 gccggccgag ggcctgctga aggagggcga cgtgctgctc tccgtggacg ggcacgcgat
     3181 cgccaacgac ggcacggtcg agttccgccc ccgtgagcgg acgagcgtgt cctactggat
     3241 ccagcagcac caggtcggcg agacggccaa cctcgacatc ctgcgggacg gggaggcgct
     3301 gccggtgcag gtgcggctga ccagccgcat ggagcaggac tggatcatcc cgttcgagga
     3361 atacgacatc ctgcccacct actacattta cggcggcatc gtgttcagcc cgctgacggt
     3421 caacctgctg cagtcgtggg gcggcaactg gtacgagaag gccccgcggg acctcgtcgc
     3481 ccggctgggc ttcaacctgg tgaccccgga acaggacgag gtcgtgctcg cgctgcgcgt
     3541 cctggccgcc gacgtcaacg agggctacca caacgtttcg tcgtggaccg tcgacaaggt
     3601 cgacggcgag aaggtccgga acctgaagca cctgatcgag ctgattgaaa agggcagcgg
     3661 ccccttcgtc gtgctggaaa acgagtgggg ccagaaactc gtgctcgacc gggagcaatc
     3721 ggagcagacc gggccgcaga tcctgggaac ctaccggatt ccgaaagacc ggtctccgga
     3781 cctcgcggcg ccctgaagaa gctcgggccg caacccggcg gcgggatcag gccagtttct
     3841 ttttcgccag gtccacgatg gacgcgaagc cgtccgggtc gtggatggcg atctcggaga
     3901 gcatcttgcg attcagccgg atgccggcct tcgtgacgcc ctccatgaaa cggctgtagg
     3961 acaggcccaa ctcgcggcac gccgcgttca ggcgcacgac ccacaggttg cggaactcgc
     4021 gcttgcggag cttgcggtgc ttggtggcct ggaccatggc gcggtccacg gcctcggtgg
     4081 ccatgcggaa ccgcttgctg cgaccgccgt aaaacccctt ggctaaaccg aggcggcgtt
     4141 tccttctccg gcgcgaggcc ggcgcgttgg tggctctgga catagtcgtt ctccttagag
     4201 gggcaggccg cggctgatcc ggccgtagtc ggcgctcgac acggtgctgc cggcgcgcaa
     4261 ctggcgcttg cgcttgcggc tcttgtggcc gagcagatgg ctgcggctgc cgcacgcgtg
     4321 cttgatgacg ccgcccgcgg tcttgtggaa ccgcttcgag acggccttcc tggttttata
     4381 ttttccggcc atgataccct ctgtatttca tccgcccgcc gcacggatcc ttgatccctg
     4441 cgcttccggg cgggaaaggg cgcgtttata cgccagatcg atccgggagt ccagcgttat
     4501 tgcgcaggcg cagtctccag cgccgcgggc gccggcttgg cctccgcggc cttgggcttc
     4561 gccccgggct tcgggcacag gagcatgaag atggcccggc cgatgaagcg gggcatctgc
     4621 tccggtgtgc cgatgtcctg gcaatccttc aggacccggt tcatcaccgc gaatccgatc
     4681 tcctcgtggg cgcgctcgcg gccgcggaag aacaggccga tcttcacgcg gtggccctcc
     4741 tggaggaact cgcgggtgtg gcgcaccttg gtctggtagt cgtggtcgcc gacgttcggg
     4801 tggaactgga cttccttgac ccgggtcgcc gactggtggc ggcgcgcctg cttgtccttc
     4861 ttctcctgct cgtacttgaa cttgccgtag tccatgatcc ggcagacggg cggctgcgcg
     4921 ttgggcgaga tctccaccag gtccatgccc tcccgggacg cccgttcgag ggcatcgcgg
     4981 gtcgtgatga cgccgatctg ctgtccgtcc ggcccgatgc accgcacctc gggcacccgg
     5041 atccggttgt tcacgcgaac gaatgtctga ataacttcct cctgcgttta cggggccccg
     5101 ccgggcccct ggttgtttct ccgcgcctgt gcttcgctca ccccggggcc aggccccggc
     5161 ggtcctcctc ggccttccac atcgtgatca gcgcgggcag gtccaccgcc ccgcggtccc
     5221 cctccgtccg gctccgcacc gcgacggtct tcgcctcggc ctcgcggccg ccggcgacca
     5281 gcatgtaggg gatcttcatc agttgcgcgt cgcggatgcg cgcgcccagc ttctccggcc
     5341 gctcgtccag gccgacgcgg aagcccgcgt cgcgcagcgc ggcggccacc gaacgggcgt
     5401 actccgcctg cttgtcggtc agcggcagca cgcgcgcctg ctcgggcgcc agccagaacg
     5461 ggaacgcccc ggcgtagtgc tccgtcagga tgccgaagaa ccgctcgatg ctgccgaaga
     5521 gcgcgcggtg gaccatgtac ggccggtggt ccttgccgtc cgcgcccgtg aaggccatgt
     5581 cgaaccgctc cggcaggttg aagtcgaact ggatcgtgct catctgccac tcgcggccaa
     5641 tggcgtcctt gacgcgcatg tcgatcttcg gaccgtagaa ggccccgccg ccggggtcca
     5701 cctcgtacgg gatgccggcc tccttcaggg cctgctccag cgagcgggtc gcctgctccc
     5761 agcgctccgg ctcgccgacg gccttgtccg ggcgcgtgga gagatacgcg gacatcgagg
     5821 agaacccgaa ggttttccac atgcggatgg cgaaatgcat cgcccgcagg acctcgtcct
     5881 cgacctgctc cggcgtgcag tagatgtgcg cgtcgtcctg ggtgaagccg cggacgcgga
     5941 agagcccggc gaggacg
//
LOCUS       DAOOTK010000163         5808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8577_length_5808_cov_5.621067, whole genome shotgun sequence.
ACCESSION   DAOOTK010000163 DAOOTK010000000
VERSION     DAOOTK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_5808_cov_5.621067"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PK634_13190"
     CDS             complement(<1..727)
                     /locus_tag="PK634_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98730.1"
                     /translation="MTDSRSAVAAALLGLATTGLAGLLPPMTGAPGDFAQRRAGAEVA
                     AVHRIPVTNEGLYRIDYPALVAAGFTNPVGNALRMYCQTQEVALYVSSPGALSASDYV
                     LFYGWGHDGYYTITNVYWLGEGGSGLRMGVADAAPLAGGTEVTATWKQVRFNRDILFG
                     RNTRPTDTNFDHWAYGQINTSSLLQATVSTDGRAPEGMAQLDAILVGVTAAAADPDHR
                     TRVRVNARVATTNFTYDADGRYFG"
     gene            complement(720..1322)
                     /locus_tag="PK634_13195"
     CDS             complement(720..1322)
                     /locus_tag="PK634_13195"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOW98731.1"
                     /translation="MARWAGAGLMLGVALVCAQEPKLARTIGAPAPAQWEIPDDPAAE
                     APVECRVRISGFELHPAEHGEVIALPGQVPGWSQAGAPDLPAVASGFRGHPQVRLAVR
                     FLRSEFEDVPDVDVAPVPSARAGEFDQPYEKPVLTYSRSPNAYAADAWWPETLVSLQE
                     AMGGTQKHVRVECRPFQYNAARKTLRFHRQIEFALEVKRD"
     gene            complement(1400..>5808)
                     /locus_tag="PK634_13200"
     CDS             complement(1400..>5808)
                     /locus_tag="PK634_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOW98732.1"
                     /translation="DRVVADADNTPETSDEEVTLVVALPPPDVFKTDNVDPVVAGGML
                     VYTVRVWNASTFDTVSNVVVVETYDAHFQFGSAAPAPMAGTSNMWVLGDLPPGYVTNI
                     VITGTVSPSTPRDATLFNTVEVFSDAGSRTVHETTDVIVPALNLTKLAGNAPDGGIEY
                     TVAGSNVVYAYVVTNTGDTHLTIAVTDNVIGVIGTVTALAPNASATLYKTSAIPASVT
                     NIGYAAGNPVTEGNADLAGASDVYDNDDAIVQVVAPALQLVKLAGNAADGAVEYILSG
                     EDVVYTYAVSNAGNTHLSSIVVTDNVLGAVGAITGPLAPGATATLYATNLNVVADVTN
                     IGVASGDPSFGDGTPIPDISNVADSDDAVVDVVAPALQLVKLAGNAADGATEYIVAGQ
                     NVIYSYAVSNAGNTYLSSILVTDDVLGVVGAITGPLAPGATATLYATNLNVVADVTNI
                     GVASGNPTDANGNDLPDISNVTDSDDAVVDVVAPAINLVKLAGNAPDGGILYVAPGAE
                     VVYTYAVSNSGDTFLGNLVVTDNVIGAVGVVAGPLAPGATATLHATSAIPASVTNLGV
                     VVAQPVSAGGTALPDIGTVTDSDDAIVLVAQLSIAKEDLPDPVHAGMPLTYTLWIANP
                     SENPVQNVVVTETYPPQFSYISANPAPDSGNNVWNLGTLQPGDVVPIVISGAVLGGTP
                     AGSILTNRITATSNVSTGSVVETTLVVDDPPPGGAEIRITKRDEMDPVQPGSNLVYTI
                     RVENFGPGIATNVFVIETYDPLFTFVGSGPAPSSGNNVWSLGNLAPDDFREITVTGWV
                     SFAATDGTLLTNVARAISDNTEPSEDDETTLVSTRPPPELFKTDGPDPVFSGGILTYT
                     ITVQNASALVVSNVTVVEAYDARFQFQSAVPAPEPGTSNVWILGDLGPVSTAAIVITG
                     IVAPDAAHGALLFNTVEVFSDSGSRTVHETTDVLAPRTLQGAFYVDCNETGVFEEDKE
                     RIQGVNVHLYRDANANSILDGDPLVDSAVTDTNGGFAFSNLWDGTWFVGWETNAAILV
                     GATQTDGGNPDVVVIAGADAAGINQGFVPAVPLRSVSGYLWVDLNANGQPDENLALQG
                     LNGVQVFLHPVVGGVTNPAIANTFTVTDGLGNQGWYEFNGLPLGEYAVVINLATVPPE
                     YLPDAASQGGEIDEECNITVGLPFVGRPTPVTLAGLTAARGEGGVRVAWETATELNNL
                     GFHVYRSDSVGGEKVRLTSGLIAGVGTGDGRSYEFVDAGADPAATLYYWVEDVSTDFQ
                     TEQHGPAVVYGEPGAGGSDSPALASFELEEQPALYRVRYEALKAAGVAIDSIDPATLK
                     VLVDGEEVAVFVSAWRGPMKAGDYILFYAEEGAAEIRTGADALRMDEVYAGPADESGD
                     VWYGVATEEGTLAFETQADIRRYLLIGFADEQAQVLDVTDAARPKLLYGFSWLQVSGQ
                     SGAYLGYDSGTPARLLAVDASAVREVEKLLQP"
BASE COUNT          857 a         2066 c         1911 g          974 t
ORIGIN      
        1 tgccgaaata tcgcccgtcg gcatcgtacg tgaagttcgt ggtcgccaca cgcgcgttga
       61 cgcggacgcg ggtccggtgg tcgggatcgg ccgccgccgc ggtgacgccg accaggatgg
      121 cgtccagttg cgccattcct tccggcgccc gcccgtccgt ggacaccgtc gcctgaagga
      181 gcgacgacgt gttgatttgg ccgtacgccc agtgatcgaa attcgtgtcc gtcggccggg
      241 tattgcgccc gaacaggatg tcccggttga accggacttg cttccaggtg gcggtgacct
      301 ctgtgccccc ggccagcggc gcggcgtccg cgacgcccat gcgcaggccg gagccgccct
      361 cgcccagcca gtacacattc gtgatcgtgt agtagccgtc gtgcccccag ccgtagaaca
      421 ggacgtagtc cgacgcggag agcgcgccgg gcgaggagac atacagcgcc acctcctggg
      481 tctggcagta cattcggagc gcgttgccga cgggattggt gaatccagcc gccaccagcg
      541 cggggtagtc gatgcggtag aggccctcgt tggtcacggg gatgcggtga acggccgcga
      601 cctcggcgcc ggcacggcgc tgggcaaaat cgcccggcgc gccggtcatg ggcggcagca
      661 ggccggccag gcccgtcgtg gccaggccca gcagcgccgc cgcaaccgct gaccgcgaat
      721 cagtcacgtt tcacttccag cgcaaactcg atttgccggt gaaaccggag ggtctttcgc
      781 gccgcgttgt actgaaaagg ccggcactcc acgcggacgt gcttctgcgt gccccccatg
      841 gcctcctgca gcgagacgag cgtttcgggc caccacgcgt ccgccgcgta cgcgttgggc
      901 gaccgggaat aggtcagcac gggcttttca taaggctggt cgaattcccc cgcgcgcgcc
      961 gacggcaccg gggccacgtc cacatccggg acgtcctcga actcggaccg gagaaagcgg
     1021 acggccaggc gaacctgcgg atggcccctg aacccgctcg ccacggccgg caggtccggc
     1081 gctccggcct ggctccagcc cggcacctgg ccgggcaacg cgatgacttc gccgtgctcc
     1141 gcggggtgaa gttcgaatcc cgaaatgcgg acccggcact cgaccggagc ctcggcggcg
     1201 ggatcgtcgg ggatttccca ttgagccgga gccggggcgc cgatcgtccg cgcgagcttc
     1261 ggttcctgcg cgcacacgag ggccacgccc agcatcaatc ccgcgcccgc ccatctggcc
     1321 atcccggtgc tcatggtcta aaaaagaggg ccgaccgtat ggggtcggcc ctcaacgccc
     1381 tcatactttt tgtaacatac tacggctgga ggagtttttc cacttcgcgg acggcgctcg
     1441 cgtcgacggc caggaggcgg gccggagtcc cgctatcgta gccgaggtag gcccccgact
     1501 gcccgctcac ctgcagccag ctgaatccgt acagcagctt cggccgcgcc gcgtcggtca
     1561 cgtccagcac ctgcgcctgt tcgtccgcga atccgatcag caggtaccgc cggatgtccg
     1621 cctgcgtctc gaacgccagc gtaccctcct ccgtcgccac tccgtaccac acgtccccgc
     1681 tttcgtccgc gggtcccgcg tagacctcgt ccatccgcag ggcgtccgcg cccgtccgga
     1741 tttccgccgc gccttcctcc gcgtagaaca ggatatagtc cccggccttc atcgggccgc
     1801 gccacgcgct cacgaacacg gccacttcct cgccgtcgac cagcaccttc agcgtcgccg
     1861 ggtcgatcga gtcgatcgcc acgcccgccg ccttcagcgc ctcataccgc acccggtaca
     1921 gcgccggctg ttcctccaat tcgaacgagg ccagcgccgg gctgtcgctc ccgcccgcgc
     1981 cgggttcgcc gtataccacg gccgggccgt gttgctcggt ttggaagtcc gtgctcacgt
     2041 cctcgaccca gtagtacagc gtcgctgccg ggtcagcccc cgcatccacg aactcgtacg
     2101 accgcccgtc gcccgtcccc acgcccgcga tcaggcccga ggtcaggcgc accttctcgc
     2161 cgccaaccga gtcggaccgg tagacgtgga acccgaggtt gttcagctcc gtggccgttt
     2221 cccacgccac ccgaacaccc ccctcgccgc gcgccgcggt caggccggcc agcgtcaccg
     2281 gcgtcggtcg gccgacgaac ggcaggccca cggtgatgtt gcactcctcg tcgatctcgc
     2341 cgccctggga cgctgcgtcc ggcaaatatt ccggtgggac ggtggcaagg ttgatcacca
     2401 ccgcgtattc acccagaggc aggccgttga actcgtacca gccctggttc cccagtccgt
     2461 ccgtcaccgt gaaggtgttg gcgatcgcgg gattggtcac gccgccgacc acgggatgca
     2521 ggaacacctg gacgccgttg aggccctgca gcgccaggtt ctcgtccggc tgcccgttgg
     2581 cgttgaggtc cacccacagg tagccgctca cggagcgcag gggcaccgcc gggacaaagc
     2641 cctggttgat gccggccgcg tccgcgccgg cgatgaccac cacatccggg ttcccgccgt
     2701 cggtctgcgt cgcgcccacc aggatggcgg cgttggtctc ccaccccacg aaccaggtgc
     2761 cgtcccacag gttggagaag gcgaagcccc cgttcgtgtc cgtcaccgcc gagtccacga
     2821 gcggatcgcc gtccaagatg ctgttggcgt tcgcgtcgcg gtacaggtga acgttgacgc
     2881 cctggatgcg ctccttgtcc tcctcgaaca ctccggtctc gttgcaatcc acgtagaacg
     2941 cgccctgcag cgtccgcggc gcgaggacgt cggtcgtctc gtgcaccgtg cggctgccgg
     3001 agtcgctgaa cacttcgacg gtgttgaaca gcagcgcgcc gtgggcggcg tccggggcca
     3061 cgatgcccgt gatcacgata gccgccgtgc tcaccggccc cagatcgccc aggatccaga
     3121 cattgctggt gccgggttcc ggcgccggca cggcggactg gaactggaac cgcgcgtcat
     3181 aggcttccac caccgtcacg ttggagacca cgagggccga cgcgttttgc acggtgatcg
     3241 tgtaggtcag gatgccgccg gagaacacgg gatccgggcc gtcggtcttg aagagctccg
     3301 gcggcggccg cgtcgagacc agcgtggtct cgtcgtcctc gctcggctcg gtgttgtccg
     3361 agatggcccg ggcgacgttg gtcagcagcg tgccgtccgt cgccgcgaag gacacccagc
     3421 cggtcaccgt gatctcgcgg aagtcgtcgg gcgccaggtt gcccaggctc cacacgttgt
     3481 tcccggagct cggcgccggg cccgacccga cgaaggtgaa cagcggatcg taggtctcga
     3541 tcacgaacac gttcgtcgcg atgcccggcc cgaagttttc gacccggatc gtgtagacca
     3601 ggttcgagcc cggttgcacg ggatccatct cgtcgcgctt ggtgatgcgg atctccgcgc
     3661 cgcccggcgg cgggtcgtcg accacaagcg tggtttccac gacggaaccc gtgctgacgt
     3721 tggacgtcgc agtgatccgg ttcgtcagga tcgaacccgc cggcgtgccg cccagcacgg
     3781 cgccgctgat cacgatcggc accacgtcgc ccggctgcag cgtccccagg ttccagacgt
     3841 tgttgccgga atccggcgcc gggttcgcgg atatgtagct gaactgcggc ggataggttt
     3901 ccgtcacgac cacgttctgc acggggttct cggacgggtt cgcgatccac agggtgtacg
     3961 tgagcggcat gcccgcatgc acgggatccg gcaggtcctc cttggcgatg ctcaactgcg
     4021 ccacgaggac gatcgcgtcg tcgctgtcgg tgaccgtccc gatatcgggc agcgcggtgc
     4081 cgcccgcgct cacgggctgc gcgacgacca cgccgaggtt cgtcacgctc gccgggatcg
     4141 cggaggtcgc atggagcgtc gcggtggcgc cgggcgccaa cggacccgcg acgacgccga
     4201 ccgcgccgat cacattgtcc gtcaccacca agttgcccag gaaggtatcg cccgagttgc
     4261 tgacggcgta agtatagacc acctccgcgc ccggcgcgac gtacaggatg ccgccgtcgg
     4321 gcgcgttacc cgccagcttg accaggttaa tggccggggc cacgacgtcc acgacggcgt
     4381 cgtcgctgtc cgtcacgttc gagatgtccg gcaggtcgtt tccgttcgcg tctgtcggat
     4441 tgccgctcgc gacgccgatg ttggtcacgt cggccacgac gttcaggttg gtcgcgtaga
     4501 gcgtcgccgt cgcgcccgga gccaacgggc cggtgatggc gccgaccacg cccagcacgt
     4561 cgtccgtcac caggatcgag ctcaagtacg tattgcccgc attgctcacg gcgtagctgt
     4621 agatcacgtt ctggcccgcc acgatgtatt ccgtcgcgcc gtccgccgcg ttgcccgcca
     4681 gcttgacgag ttgcagcgcc ggggcgacca cgtccaccac ggcgtcatcg ctgtccgcca
     4741 cgttcgagat gtccgggatc ggcgtgccgt cgccgaagct cgggtcgccg ctcgcgacgc
     4801 cgatgttggt cacgtcggcc acgacgttca ggttggtcgc gtagagcgtc gccgtcgcgc
     4861 ccggggccaa cgggccggtt atggcgccga ccgcgcccag cacgttgtcc gtcacgacga
     4921 tcgagctcaa gtgcgtgttg cccgcgttgc tcaccgcata cgtgtagacc acgtcttcac
     4981 cggaaaggat gtattccacc gccccgtccg ccgcgttgcc cgccagcttg acgagctgca
     5041 gcgccggggc cacgacctgc acgatcgcgt cgtcgttgtc gtacacgtcc gaggcgcccg
     5101 ccaggtccgc gttgccctcg gtcacgggat tgcccgcggc gtaaccgatg ttcgtcacgc
     5161 tcgccgggat ggcggaggtc ttgtacagcg tggcgctcgc gttcggcgcc agggccgtga
     5221 ccgtgccgat cacgccgatg acgttgtccg tcaccgcgat cgtcaggtgc gtgtcgccgg
     5281 tgttggtgac gacgtacgcg tacaccacgt tgctgccggc gaccgtgtac tcgatgccgc
     5341 cgtccggggc gttcccggcc agcttggtca ggttcagcgc gggcacgatg acgtcggtcg
     5401 tctcgtgcac cgtgcggctg ccggcgtcgc tgaacacctc gacggtgttg aacagcgtcg
     5461 cgtcccgcgg cgtggacggc gaaaccgtcc cggtgatgac gatgttcgtc acgtagcccg
     5521 gcggcaggtc gccgagcacc cacatgttgc tggtcccggc catcggggcc ggcgccgcgg
     5581 agccgaactg gaagtgggcg tcataggtct cgaccacgac cacgttggag accgtgtcga
     5641 acgtcgaggc gttccacacg cgcacggtgt agaccagcat cccgcccgcg acgacgggat
     5701 ccacgttgtc ggtcttgaag acgtccggcg gcggcagggc caccaccagc gtgacctctt
     5761 cgtcgctggt ctccggcgtg ttgtcggcgt ccgccacgac ccggtcca
//
LOCUS       DAOOTK010000164         5806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8580_length_5806_cov_3.354895, whole genome shotgun sequence.
ACCESSION   DAOOTK010000164 DAOOTK010000000
VERSION     DAOOTK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8580_length_5806_cov_3.354895"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK634_13205"
     CDS             <1..769
                     /locus_tag="PK634_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOW98733.1"
                     /translation="GARVERILVEGGRAVGVALQDGETRRADIVVSAADGHATIFDML
                     KGRFTNGRIRRKYETYKTFPSYLQVSLGVGADLKGEPGTIVLFPDEIIRLDPDTTAES
                     LTCRIFHFDPTMAPPGKTAVTCLLPTYNEPYWSDLRRQDPARYEAEKLRVAAAVTAAF
                     EARFAGARGRIEVVDVATPATVVRYTGNWKGSMEGWLYTPATGIRPLPCVLPGLRNFY
                     MVGQWIAPGGGLPSALMTARSVSKRIARESGIRWKLA"
     gene            complement(783..2546)
                     /locus_tag="PK634_13210"
     CDS             complement(783..2546)
                     /locus_tag="PK634_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOW98734.1"
                     /translation="MNPGMESAPGAWSRPSGRHIPAAVAAAVLSLAAHWAAWERAPFL
                     PFGALARVRDEVRFPSIRLNEVQPTPTEPLQRPARFRPEDPDRQADLAEETADWTEQV
                     REALPATPDLSGIKLAGESATLETPGAPPARERWEPRQEILQVEEELYAEEISALPRR
                     YTPAVPRVAAAPDLAAPSPIEWAAAASAAGTAPVESGEAGAWLRQTPNPAQSPGGPAG
                     ESAPPAGSAEESALLDEKLPDVTALKPVEQMLGIEVRTWSPPDEPENAYFELSVRREG
                     ETLLPVLPKDVLLIQDCSESMTQAKLNYCKEGLQRWIGQFDAADRLDLLAFRDTTERL
                     FGGWTELTTVNRSIAAGYIERLIARGRTDVYASLQQVLALERDPGRPVLAVLVTDGRP
                     TMGMVDSSDIIEAFTWDSAGSVSMFCVGGGTRVNRFLLDLLGYKNRGDSLVVEDSRNI
                     PDALQELARQVSRPVLTDLTYRFSGLPAEDIFPRTLTHLYLDRPLVIYGRCPAGAPRV
                     VFQVVGQARGQKADMVFEWDWSRVQPGDESLRTKWAWQRIYHLIGEHVREPRQEILNE
                     IHATANRYGLIVPYGTDIPTR"
     gene            complement(2551..4023)
                     /gene="rho"
                     /locus_tag="PK634_13215"
     CDS             complement(2551..4023)
                     /gene="rho"
                     /locus_tag="PK634_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOW98735.1"
                     /translation="MPEPRGRYRRTRTPHAGSSHAPVSEPPLRRQHEPAPPMPPPPPP
                     PVAPPVVNGNLDPAAGNAAPPAPPLQGAGRTLHLFDLQVQSVPDLLKMADSYGLQDLG
                     ALRKHELIFEILKANGRLNGAMYGRGVLEILPDGFGFLRSPNYNYLPCPEDIYVSPSQ
                     IRRFALRTGDLVDGEIRAPKDKERFFALLKVDRINESEPDAQRHKVPFENLTPLFPDR
                     RFVLETTKEEVSMRVLDLLTPIGMGQRGLIVAPPRTGKTVLLQKIANSITANNPKAKL
                     IVLLIDERPEEVTDMERNTKAEVLSSTFDEPPERHVQVAEIVIEMAKRMVECGRDVVI
                     LLDSITRLARAYNTLQPHSGKILSGGVDANALHKPKRFFGAARNIEGGGSLTIVATAL
                     VDTGSRMDEVIFEEFKGTGNMELGLDRHLVDKRIFPAINIEKSGTRKEELLLHPDELQ
                     KIWLMRKALNGVPPVEAMELLVNRLKKTGSNAEFLLTVQG"
     gene            complement(4027..4647)
                     /gene="coaE"
                     /locus_tag="PK634_13220"
     CDS             complement(4027..4647)
                     /gene="coaE"
                     /locus_tag="PK634_13220"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOW98736.1"
                     /translation="MKTRRASSPAVIGLTGGLACGKSEVGRILARLGAAVLDTDEVTR
                     ALQKPGRPVFRAVVRRFGRSVLTPAGELDRRALARRVFGDPASRRALEAIVHPAVFAE
                     AEKWMRARRRERKPAVVIVPLLYEVGRTKGWDAVWCVTSPKPAAMARLKARGLTAAEA
                     RARWAAQMAPAEKARRADVVIRNNGSLDNLEKTVRAVWNQTTREEP"
     gene            complement(4634..>5806)
                     /locus_tag="PK634_13225"
     CDS             complement(4634..>5806)
                     /locus_tag="PK634_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98737.1"
                     /translation="RAAPPARSALATVALLLAPALAFSWGGAYRQAYGHFLDLLVAKL
                     MCLNRKPADPGLLSFDARILWVPGLNSATWALASRFFPVMLPLTLFAIAVLLFKKSFR
                     SDPGLARHVFFISASFATFVFFVRFHVLVAVFAAGALGLAAASLGEGRRWYRWLGVLF
                     LAGMGAEAAHTLDMPERWGRGQVYYKEMEELTGWMKAHVRPEPVLANFGISASILAYA
                     ECPVLLHPKFESKELRERVRAYGEKLFLGTERELRDWADGLGARYLVFGRGEFAAVEP
                     QRQMRYFVNALNPPPHAAARRLEQEPGQARWFRMVWENRKYRVFRILTRAEEGAARRY
                     GIEAEEALQRGELDRAEEQATSALLLDAREETAQKVLQHVGSLRRQGFRSEGDENP"
BASE COUNT          875 a         2031 c         1971 g          929 t
ORIGIN      
        1 cggcgcccgg gtcgagcgca tcctggtcga gggcgggcgc gccgtcggcg tcgcgctcca
       61 ggacggcgaa acgcggcgag cggacatcgt tgtgtcggcg gccgacggcc atgcgacgat
      121 tttcgacatg ctgaaaggcc ggttcacgaa cggccggatc cggaggaagt atgaaacgta
      181 caagaccttc ccttcctacc tgcaggtgtc gcttggcgtc ggggccgacc tgaagggcga
      241 accgggaacg atcgtgctct tcccggatga aatcatccgg ctcgatcctg acacgaccgc
      301 ggaatcctta acctgtcgca tttttcactt cgatccgacg atggcgccgc cggggaagac
      361 cgccgtgaca tgccttctcc ccacttacaa cgagccgtac tggagcgatt tgcgcaggca
      421 ggatcccgcc cggtatgaag ccgagaagct ccgcgtggcc gcggcggtca cggccgcgtt
      481 cgaggcgcgg tttgccgggg cccggggccg gatcgaggtg gtcgacgtcg cgacgccggc
      541 gaccgtggtc cgttacaccg gcaactggaa gggcagcatg gaaggctggc tgtacacgcc
      601 ggccacgggc atccggccgc tcccgtgcgt cctgccggga ctccggaatt tctacatggt
      661 cggccagtgg atcgcgcccg gcgggggcct gccgtcggcc ctgatgacgg cccgcagcgt
      721 gtcgaagcgg atagcccggg aatccggcat ccgctggaaa ctggcgtagg agcgcggcgc
      781 ccctaccgcg tgggaatatc ggtgccgtac ggaacgatca ggccgtagcg attggccgtg
      841 gcgtgaattt cgttgaggat ttcctggcgc ggctcgcgga cgtgctcgcc gatgaggtgg
      901 tagatcctct gccaggccca cttcgtccgg agggactcgt cgcccggctg cacccggctc
      961 caatcccact cgaagaccat gtccgctttc tgcccgcgcg cctgccccac cacctggaag
     1021 accacgcgcg gggcgccggc cgggcaccgc ccgtagatca ccagcgggcg gtcgaggtag
     1081 agatgcgtca gcgtccgcgg aaagatgtcc tcggccggga ggcccgagaa ccggtacgtg
     1141 agatcggtca gcaccgggcg gctgacctgg cgggcgagtt cctgcagggc gtccgggatg
     1201 ttccggctgt cctcgaccac gagcgagtcg ccgcggttct tgtatcccag caggtccagc
     1261 agaaaccggt tcacgcgcgt gccgccgccg acgcagaaca tggacaccga tcccgcgctg
     1321 tcccacgtga aggcctcgat gatatccgag ctgtccacca tgcccatcgt gggccgcccg
     1381 tcggtgacca gcacggcgag gaccggccgt ccgggatcgc gctcgagggc gagaacctgc
     1441 tgcagggagg catatacatc cgtccggccc ctcgcgatca gccgctcgat atagccggcc
     1501 gcaatggaac ggttgaccgt ggtcagttcg gtccaacccc cgaacaggcg ctccgtggtg
     1561 tcgcggaacg cgagcaggtc gagccggtcg gccgcgtcga attggccgat ccatcgctgc
     1621 aggccttcct tgcagtagtt cagcttggcc tgggtcatgc tctcggaaca gtcctgaatc
     1681 agcagcacgt ccttgggcag gacgggcagg agggtttcgc cttcgcgccg gacgctcaac
     1741 tcgaaatacg cgttctccgg ctcatccggc ggcgaccacg tccgcacctc gatgccgagc
     1801 atctgctcga ccggcttgag cgcggtgacg tcggggagtt tttcgtccag cagcgcggac
     1861 tcttccgcgg agccggccgg gggggcggat tcccccgcgg gcccgcccgg cgactgggcc
     1921 ggattgggag tctgccgcaa ccacgcgccg gcctcgccgg attccacggg agctgttcca
     1981 gcggcggacg cggcggcagc ccactcgatg ggcgaaggcg cggccaggtc cggggcagcg
     2041 gccacgcggg gaacggccgg agtgtagcgg cgcggcagcg cgctgatctc ctcggcgtag
     2101 agttcttcct cgacctgcag gatttcctgg cgcggctccc agcgctcgcg cgccggcggg
     2161 gcgcccggcg tctccagggt ggcggactcg ccggcgagct tgatcccgga caggtccggt
     2221 gtcgccggca gtgcctcgcg gacctgctcg gtccagtcgg cggtctcctc cgccaggtcg
     2281 gcctgccggt ccggatcctc cgggcggaac cgggcgggcc gctggagcgg ctcggtcggc
     2341 gtcggctgca cctcattcag gcggatggag gggaagcgca cctcgtcgcg cacgcgcgcc
     2401 agggcgccga acgggaggaa cggggcccgc tcccaggccg cccagtgcgc ggccagcgag
     2461 aggaccgcgg cggccacggc cgccgggata tggcggccgg acgggcggct ccatgcgccc
     2521 ggcgcgctct ccatcccggg attcatcccg tcatccctgc acggtcagga ggaattccgc
     2581 gttgctgccc gtcttcttca gccggttgac cagcagttcc atggcctcca cgggcggcac
     2641 gccgttcagg gccttgcgca tgagccatat cttctgcagc tcgtccgggt gcagcagcag
     2701 ctcttccttg cgcgtgcccg acttctcgat gttgatggcc gggaagatgc gcttgtccac
     2761 gaggtgccgg tccaggccca gctccatgtt gccggtgccc ttgaactcct cgaagatcac
     2821 ctcgtccatg cggctgcccg tatccaccag ggccgtcgcg acgatggtca ggctgccgcc
     2881 gccctcgatg ttgcgggccg ccccgaagaa gcgcttgggc ttgtgcaggg cgttggcgtc
     2941 cacgccgccg gacaggatct tgccgctgtg cggctgcagg gtgttgtagg cgcgcgccag
     3001 ccgcgtgatg ctgtcgagca ggatcacgac gtcccgtccg cactccacca tgcgcttggc
     3061 catttcgatg acgatctcgg ccacctgcac gtgccgctcg ggcggctcat cgaacgtcga
     3121 gctcaagacc tcggccttcg tgttgcgctc catgtccgtc acttcctcgg gccgctcgtc
     3181 gatcagcagg acgatgagct tggccttggg gttgttggcc gtgatgctgt tggcgatctt
     3241 ctgcagcagc acggtcttgc cggtgcgcgg cggggcgacg atcaacccgc gctggcccat
     3301 gccgatcggg gtcagcaggt ccagcacccg catggagacc tcctccttcg tcgtctcgag
     3361 cacgaagcgc cggtccggga acaggggcgt caggttctcg aacgggacct tgtgccgctg
     3421 ggcgtccggt tcgctctcgt tgatccggtc gaccttcagg agggcaaaga accgctcctt
     3481 gtctttcggc gcgcgaattt cgccgtccac caggtcgccg gtccgcaggg cgaagcggcg
     3541 gatctgggag ggcgagacgt agatgtcctc ggggcacggc aggtagttgt agttcggcga
     3601 gcgcaggaac ccgaaaccgt cgggcaggat ttccagcacg ccgcggccgt acatggcgcc
     3661 gttgaggcgt ccgttggcct tgaggatctc gaagatcagc tcgtgcttgc gcagcgcgcc
     3721 gagatcctgg agcccgtagc tgtccgccat cttcagcagg tcggggacgg actggacctg
     3781 gaggtcgaac aggtgcagcg tgcggccggc gccctgcagg ggcggcgcgg gcggcgcggc
     3841 gttgccggcg gccggatcca ggttgccgtt caccaccggc ggggcgaccg ggggcggcgg
     3901 cggcgggggc atcggcggcg cgggctcgtg ctgccgccgc agcgggggtt cggacacggg
     3961 cgcgtggctg ctgccggcgt gcggggttct cgtcctgcgg tatcgtcctc ggggttcagg
     4021 catggtctac ggctcctctc tcgtcgtttg gttccatacg gcccggacgg ttttctccag
     4081 attgtccagg ctcccattgt ttcgaatcac cacatcggcc cggcgggctt tctccgccgg
     4141 cgccatctgg gccgcccacc gggcgcgggc ttccgcggcc gtcagcccgc gcgccttgag
     4201 gcgcgccatc gcggccggtt tcggcgacgt cacgcaccac acggcgtccc agcccttcgt
     4261 ccgccctacc tcgtagagca gcggcacgat gaccaccgcc ggcttccttt cgcgacggcg
     4321 ggcgcgcatc cacttctccg cttccgcaaa caccgcgggg tgcacgatcg cttccagcgc
     4381 ccgacgggag gccggatcgc cgaacacccg ccgcgccagc gcccgccggt ccagttctcc
     4441 ggccggcgtc agcacgctcc ggccgaaccg ccggacgacg gcccggaaca ccggccgccc
     4501 cggcttctgc agcgcgcgcg tcacctcgtc ggtgtccaac accgcggcgc ccaaccgggc
     4561 cagtatccgg ccgacctcgc tcttgccgca ggccaggccg cccgtcaggc cgatcaccgc
     4621 gggggaggac gccctacggg ttttcatccc cctccgaccg gaatccctgc cgacgaagcg
     4681 atccgacatg ctgcaaaacc ttctgtgccg tttcctcgcg ggcatccagg agcagcgcgg
     4741 atgtcgcctg ctcctcggcc cggtccaact cgcccctctg cagggcctct tccgcctcga
     4801 tcccgtaccg ccgcgccgcg ccctcctcgg cccgggtgag gatgcggaac acgcggtact
     4861 tccggttctc ccacaccatc cggaaccacc gggcctggcc gggctcctgc tccagccgcc
     4921 gcgccgccgc gtgggggggc ggattcagcg cattcacaaa gtagcgcatc tgccgctgcg
     4981 gctcgaccgc ggcaaactcg ccccggccga agaccaggta ccgggcgccc agcccgtccg
     5041 cccagtcccg cagctcccgc tcggtgccca ggaacagctt ctcgccatag gcccggaccc
     5101 gctcccggag ttccttcgac tcgaacttcg ggtgcaggag caccgggcac tccgcgtagg
     5161 ccagtatcga ggcgctgatt ccaaaattcg ctaggacggg ttcaggcctc acatgcgcct
     5221 tcatccatcc cgtcagctct tccatttcct tgtaatacac ctggccgcgg ccccatcgct
     5281 cgggcatgtc gagcgtatgc gccgcttcgg cgcccatccc ggccaagaac aggacgccta
     5341 gccatcggta ccaccggcgg ccttctccca gggaagcggc cgccagcccc aacgcgcccg
     5401 ccgcgaatac ggccacgagc acatggaatc gaacgaaaaa aacaaacgtc gcgaatgaag
     5461 ccgaaatgaa aaaaacgtgc cgggccagac cgggatcgct gcgaaatgat ttcttgaata
     5521 acaataccgc gatggcgaac aaagtcaagg gcagcatcac gggaaaaaac ctggaggcga
     5581 gggcccacgt cgccgagttc aggccgggca cccacaagat gcgggcgtcg aagctcaaca
     5641 ggccggggtc ggccggcttg cggttcaggc acatcagctt ggccaccagc aggtccagga
     5701 aatgaccata ggcctggcgg taggccccgc cccaggagaa cgccagcgcg ggggccaaca
     5761 acagcgccac ggtggccagg gcggaacggg ccggcggcgc ggcgcg
//
LOCUS       DAOOTK010000165         5610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_8989_length_5610_cov_5.329433, whole genome shotgun sequence.
ACCESSION   DAOOTK010000165 DAOOTK010000000
VERSION     DAOOTK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8989_length_5610_cov_5.329433"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..3289)
                     /locus_tag="PK634_13230"
     CDS             complement(167..3289)
                     /locus_tag="PK634_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOW98738.1"
                     /translation="MILSNYAIKFRTAVFVFAIVLVIAGSVSYLRLPREGTPDITIPY
                     VFISAYYEGTAPEEMEKLVTIPLEKQLNDVENIKEMRSTSGENYCSISIEFLAGGNID
                     LAKQRVKDKVDLAKPDLPDDLDEPVVDAFNFSSDFPVYIFTLSGPDDLDRLKNLAEDL
                     QDQIEQLSGIKQAVLSGTREREIRVEIDLPRMIAYRIPLGLVMQRIAQENLTISAGNI
                     EMAGDKFQVRIPGEFKLVPEIRRILLTERDGRPVYLTDIAAVSDTFKDVASISRLNGK
                     PCVSLSLKKRSGENAVALLKQVKQIIGQTQLPPDVVLTEVMDMSEYIDSMIKELENNV
                     ASGFLLVVLVLLVFMGVRNALFVGLAIPFSMLMAFVLMAAQGATLNMIVLFSLVLAVG
                     MLVDNAIVIVENTYRHRTLGESRIVAARHGASEVAWPVITSTLTTLVAFWPLLAWPDV
                     MGQFMSFLPKTLIVTLSASLFVAMVINPAICSAFISARPRDQHEKPHPFVRGYERLLR
                     AALRHRVPVLLLGFAFLILSVQVYAWRELGVELFPDVEPRNCTVNLKFPQGTSIERTD
                     AVLRDAERKLAKYPDVKFFLTTVGSSGDMFGGGAEESHRGTIHVEFLKAGERQTNSLA
                     LLDAIRSDIGIIAGAELKVEREEEGPPTGAPVSIELSGDDFDTLESYAGEIMRAIESV
                     PGLVDLQSDLEKALPEIQFHVDRARAALLGLDTGTIGTFLRMAIYGLESSRFRADEDE
                     YDITLRLPEGQRNTMNLLEQVFIPAENGQNVPLSSLGQLEYTGGRGAIQRKNQKRVVT
                     ITGNNAGRGVDKILPDIQTRVADLNLPRGYRVEYTGDTEEMRESGAFLMRAFGIASGL
                     ILVILVIQFNSVLLPLIIFFSVILSMIGVMWGLLICGMKFGVIMTGVGVISLAGIVVN
                     NAIVLIDCIEQRRAEGESMAESIVTAGRLRLRPVLLTASTTVLGLIPMAVGWSLEFHS
                     WPPKLIAGAESSAWWAPMAVAVIFGLSLATLLTLILVPAMVSIADSVAAAFRKRFAPV
                     DDLNHTPPPA"
     gene            complement(3304..4413)
                     /locus_tag="PK634_13235"
     CDS             complement(3304..4413)
                     /locus_tag="PK634_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOW98739.1"
                     /translation="MNTPPAPRSRKGSWLVWGALLAVVALIVLFGALGKKPEEKPSPP
                     EKAYAVRVEPVRPRTVSDEIQLPGVVEPFKEAVLAAERAGRILALEAEKGAAVEEGQV
                     LLRLDGRVWDAQVRRAEVESRDAERDLKRYRELEKTGAVSPSDYEAIERRRDMANITR
                     DEAKVFLSQCEVRAPFPGVLNDRLVEVGDYAGEGQAVLRLIQTDRVKIGFDVPEQDIP
                     AAKKGDALAFTLAALPGRAFTGAVTYVSGRAARESNSFRVELETANDGGELKPGMIAQ
                     VTLVRRVRPDAIVVPLAAIVPRKGEHVVFVVEGDRAVRRRVQIDALAGHDAALSAGLS
                     AGEALVVEGHRGLQDGRLVEIVGADTPAPPLNPGP"
     gene            complement(4410..>5610)
                     /locus_tag="PK634_13240"
     CDS             complement(4410..>5610)
                     /locus_tag="PK634_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW98740.1"
                     /translation="SAILKAHEDVRIAETRITQVRSAVLPHLSAGAGYTRLGEVSAFD
                     FDGERVEMGRLDNYSADAEISQLLYSGGSVQAGLKAARVYRDMARFRLEQVAHDLARD
                     IRTGFTDILLAQAAVKVQEESVAQIEDLVRQAEARVKKGTAPEFDLLSARVRLANEMP
                     VLIRARKDLELAKAAFRNLIQLEDGPFEVAGELEYRPEKKALADWQAAGRRDRPELLY
                     QQRAIDLARLGIRAEQGTLQPQVRAHAAYQGLNPESGTAEDAWDWGWNAGVTLDWDLF
                     DGGLRRAVILEKNLELAKSRADLDILQRNVELEIQQQHLELATADETVAASRETVALA
                     EKSLAIALARYETGLSTYLEYAAAQLSVSTARLTWLTALRDHMNALARLECASGLGPD
                     LAKDDAR"
BASE COUNT          865 a         1863 c         1946 g          936 t
ORIGIN      
        1 gagcaggtag tcccagtcgg cggcggtcgg agggctgtcg tagtcccagc gctcctcgtc
       61 ggatcgcttg aactgccgct tgatgtgctg ccgggccacc gacacccgca cgaggcgctt
      121 ggccccgtcg ggcaggcgcg tcgtccattc gatctgcttc ttcatgtcag gcgggcggcg
      181 gcgtgtggtt caggtcatcg accggggcga accgcttccg gaaagccgcc gcgacggaat
      241 ctgcgatgct aaccatggcg ggcaccagga tcagcgtaag cagggtggcc agggacaggc
      301 cgaagatcac ggcgacggcc atgggcgccc accacgcgct ggattccgcg ccggctatga
      361 gtttcggcgg ccacgagtgg aattccaggc tccaaccgac cgccatcggg atcaggccca
      421 gcaccgtcgt ggacgcggtc aggaggacgg gccgcaagcg aagccgtccg gccgtcacga
      481 tggactcggc catgctctcg ccctcggctc gccgctgctc gatgcagtcg atgagcacga
      541 tggcgttgtt gacgacgatg ccggccaggc tgatgacgcc gacgccggtc atgatcacgc
      601 cgaacttcat gccgcagatc agcaggcccc acatgacgcc gatcatggag aggatcacgg
      661 agaagaagat gatcagcggc aggaggacgg agttgaactg gatcacgagg atgaccagga
      721 tgaggccgga ggcaatgccg aaggcgcgca tcaggaaggc gccggactcg cgcatctcct
      781 ccgtgtcgcc ggtgtactcc acgcggtagc cgcggggcag gttcaggtcg gcgacgcgcg
      841 tctggatgtc gggcaggatc ttgtccacgc cgcgccccgc gttgttgccg gtgatggtga
      901 ccacgcgttt ctggttcttg cgctggatgg cgccgcgacc gccggtgtac tcgagttggc
      961 ccagcgagga gaggggcacg ttctgcccgt tctcggcggg gatgaagact tgctcgagca
     1021 ggttcatggt gtttcgctgg ccctcgggca ggcgcagggt gatgtcgtac tcgtcctcgt
     1081 ccgcccggaa ccggctgctc tcgagcccgt agatggccat gcgcaggaag gtgccgatgg
     1141 tccccgtgtc gagcccgagc agggcggccc gggcccggtc cacgtggaac tggatctcgg
     1201 gcagcgcctt ctccaggtcg ctttgcaggt ccacgaggcc cgggaccgac tcgatcgcgc
     1261 gcatgatctc gcctgcgtag gattccagcg tgtcgaaatc gtcgcccgac agctcgatgg
     1321 agaccggcgc gccggtgggg gggccttcct cctcgcgttc cacctttagt tccgcccccg
     1381 cgatgatccc gatgtcgctc cggatcgcat ccaacaacgc cagcgagttg gtttgcctct
     1441 cccccgcctt caggaattcc acgtggatcg tgccgcggtg ggactcctcc gcgccgccgc
     1501 cgaacatgtc gccgctgctc ccgacggtgg tcaggaagaa cttgacgtcc gggtacttcg
     1561 ccagcttccg ttcggcgtcg cgcaggacgg cgtcagtccg ctcgatgctg gtgccctgtg
     1621 ggaacttcag gttcacggtg cagttgcgcg gctcgacgtc ggggaacagc tcgacgccca
     1681 gctcgcgcca ggcatagacc tgcacggaca ggatcaggaa ggcgaagccc agcagcagga
     1741 cggggacgcg gtgccgcagg gcggcgcgga gcagtcgctc gtacccgcgc acgaaggggt
     1801 gcggcttctc gtgctggtcg cggggccgcg cgctgatgaa ggccgaacag atcgcgggat
     1861 tgatgaccat ggccacgaag agcgaggcgg agagggtcac gatcagggtc ttcggcagga
     1921 agctcatgaa ctggcccatg acatcgggcc aggcgaggag cggccagaag gcgaccagcg
     1981 tggtcagggt cgacgtgatg accggccagg cgacctcgga ggccccgtgg cgggcggcga
     2041 cgatgcgcga ctcgcccagg gtccggtgtc ggtaggtgtt ctccacgatg acgatggcgt
     2101 tgtccacgag catgccgacg gccagcacga ggctgaagag cacgatcatg ttcagcgtcg
     2161 cgccctgggc ggccatgagc acgaaggcca tgagcatgga gaaggggatc gccaggccca
     2221 cgaacagggc gttgcgcacc cccatgaaga ccaggaggac caggaccacg agcaggaagc
     2281 cggaggccac gttgttctcc agttccttga tcatggagtc gatgtactcc gacatgtcca
     2341 tgacctcggt caggacgacg tcgggcggga gctgggtctg cccgatgatc tgcttgacct
     2401 gcttgagcag ggccaccgcg ttctcaccgc tgcgcttctt gaggctcaag gaaacgcagg
     2461 gcttgccgtt gagccgcgag atggaggcga cgtccttgaa cgtgtcggac accgccgcga
     2521 tgtcggtgag gtacacgggc cgcccgtccc gctcggtcag caggatgcgg cggatctccg
     2581 gcaccagctt gaactcgccc gggatccgca cctggaactt gtcgccggcc atctcgatgt
     2641 tgccggccga gatggtcagg ttctcctggg cgatgcgctg catgaccagc ccgagcggga
     2701 tgcggtaggc gatcatccgg ggcaggtcga tctcgacccg gatctcgcgc tcgcgcgtgc
     2761 cgctcaagac ggcctgcttg atccccgaga gctgctcgat ctggtcctgc aggtcctcgg
     2821 ccaggttttt caggcggtcc aggtcgtccg ggcccgacag ggtgaagata tagacgggaa
     2881 aatccgagct gaagttgaac gcgtccacga cgggctcgtc gaggtcgtcg ggcagatcgg
     2941 gcttcgcgag gtccaccttg tccttgacgc gctgcttggc caggtcgatg ttgccgccgg
     3001 cgaggaactc gatcgagatg ctgcagtagt tctcgcccga ggtggagcgc atctccttga
     3061 tgttctccac gtcgttgagc tgcttctcca gcgggatggt gaccagcttc tccatttcct
     3121 ccggcgccgt gccctcgtag taggcgctga tgaagacata ggggatagta atgtccggcg
     3181 tgccctcgcg gggcagccgc aggtagctca ccgagccggc gatgaccagc acgatggcga
     3241 acacgaagac cgccgtgcgg aacttgatgg cgtagttgga gaggatcatg gcggaaatgg
     3301 ggttcagggt ccggggttca gggggggcgc cggagtgtcc gccccgacga tctcgaccag
     3361 ccgcccgtct tggaggccgc ggtggccctc gaccacgagg gcttcgcccg ccgagagacc
     3421 ggcggagagc gccgcgtcat gcccggccag cgcgtcgatc tgcacgcgcc ggcgcaccgc
     3481 gcggtcgccc tccacgacga agaccacgtg ctcgcccttg cgcggcacga tggccgccag
     3541 cgggaccacg atggcgtccg gccgcacgcg ccggaccagc gtgacctgcg cgatcatgcc
     3601 cggcttgagt tcgccgccgt cgttggccgt ttccagttcc acgcggaaag aattgctttc
     3661 ccgggcggcc cggccggaca cgtaggtgac cgcgccggtg aacgcgcggc ccggaagggc
     3721 cgccagcgtg aaggccagcg cgtcgccctt cttcgcggcg gggatatcct gctcgggcac
     3781 gtcgaacccg atcttgaccc ggtcggtctg gatcagccgc agcacggcct ggccctcgcc
     3841 ggcgtaatcg ccgacctcga ccaggcggtc gttcaggacg cccgggaacg gcgcgcgcac
     3901 ctcgcattgg gagaggaaga ccttcgcctc gtcgcgggtg atgtttgcca tgtcccgccg
     3961 ccgctcgatg gcctcgtagt cgctggggga aaccgcgccg gttttctcca actcgcggta
     4021 ccgcttcagg tcgcgctcgg cgtcgcggct ttccacttcc gcccggcgca cctgggcatc
     4081 ccacacccgg ccgtccagcc ggagcaggac ctggccttcc tccacggcgg cgcccttttc
     4141 cgcctccagc gcgaggatgc ggcccgcgcg ctcggcggcc agtaccgctt ccttgaacgg
     4201 ctcgaccacg cctggaagct ggatctcgtc ggacacggtc cggggccgca cgggttccac
     4261 gcggacagcg taggccttct ccggcggcga tggcttttcc tccggcttct tgccgagcgc
     4321 cccgaacagc acgatcagcg cgacgacggc cagcagggcg ccccaaacga gccaggaacc
     4381 tttgcgagag cggggagcgg gaggagtgtt catcgggcgt catcctttgc aaggtcgggg
     4441 cccaggccgc tggcgcattc caggcgggcg agggcgttca tgtggtcgcg cagcgccgtc
     4501 agccaggtca ggcgcgccgt gctcacggaa agctgggccg cggcgtattc caggtaggtg
     4561 gacaacccgg tctcgtaccg cgccagggca atggcgaggc tcttctcggc cagcgcgacg
     4621 gtctcgcggc tcgcggcgac cgtctcgtcc gccgtggcca gttcgaggtg ctgctgctgg
     4681 atctccagct cgacgttgcg ctgcaggatg tccaggtcgg cccgggactt ggccaattcc
     4741 aggttctttt cgaggatcac ggcccggcgc aggccgccgt cgaagaggtc ccagtccagc
     4801 gtcacgccgg cattccaacc ccagtcccac gcgtcctccg ccgtgccgct ctccggattc
     4861 agcccctggt acgcggcgtg ggcgcgcacc tgcggttgca gcgtgccctg ctcggcccgg
     4921 ataccgagcc gcgcgaggtc tatcgcccgc tgctggtaga ggagctcggg gcggtcccgc
     4981 cggcccgccg cctgccagtc ggcgagggcc tttttctcgg gccggtactc gagttcgccg
     5041 gccacctcga atggcccgtc ttccagttgg atcaggttgc ggaaggcggc cttggcgagc
     5101 tcgaggtcct tgcgcgcccg gatcaacacg ggcatctcgt tggcgagccg cacgcgcgcg
     5161 ctcaagaggt cgaattccgg ggcggtgccc ttcttcaccc gcgcctccgc ctgccggacc
     5221 agatcctcga tctgggcgac ggactcctcc tggaccttca cggcggcctg ggccagcagg
     5281 atgtcggtga acccggtccg gatgtcgcgc gccaggtcgt gggccacctg ctcgaggcgg
     5341 aatcgcgcca tgtcccggta aacccgcgcc gccttcagcc ccgcctgaac cgagccgccg
     5401 gagtagagca gttggctgat ttccgcgtcg gccgagtagt tgtccagccg ccccatctcg
     5461 acccgttcgc cgtcgaaatc gaaggcggac acctcgccca gccgcgtgta cccggccccg
     5521 gcggaaaggt gcggcaggac ggcggaccgc acctgcgtga tgcgggtctc ggcgatcctc
     5581 acgtcttcgt gcgccttcag gatcgccgac
//
LOCUS       DAOOTK010000166         5458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9331_length_5458_cov_5.322784, whole genome shotgun sequence.
ACCESSION   DAOOTK010000166 DAOOTK010000000
VERSION     DAOOTK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_5458_cov_5.322784"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK634_13245"
     CDS             complement(<1..259)
                     /locus_tag="PK634_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOW98741.1"
                     /translation="MRILSGIQPSGKLHLGNYFGMMKPILDLQKEGEVFLFIADYHAL
                     TSVTDPRAMRQGSIDVALDFLACGLDTEKTLFYRQSDIPEVT"
     gene            436..915
                     /locus_tag="PK634_13250"
     CDS             436..915
                     /locus_tag="PK634_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98742.1"
                     /translation="MKKIIGMAVLVLALSAGGAMANNGWGLYGAWWEGGDAFGPGFKL
                     TFELVPALQFDFHLAYFNDVGDGDTDLSVTPLEFGLAMNIPVSDQLKFTVGGGPSYNF
                     VDDGDADDEIGGYIGGSLEYSPYKDVALFVEARYIFLDAGDEDLNGPGLNAGLMVTW"
     gene            1033..1755
                     /locus_tag="PK634_13255"
     CDS             1033..1755
                     /locus_tag="PK634_13255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOW98743.1"
                     /translation="MLVTGFRVKLYLYRAVQAFYRLIEVGQIHPVRELKLRALQRTTD
                     YIEKHALDAVGLDTQRDLIDYSLREVRVPGFYLEFGVFTGGTIRHIAKQLPAGQLIHG
                     FDSFEGLPEAWSGFDLGKDTFNLKGKLPKVPPNVVLHKGWFKDTIPAWLKENSGKIAF
                     LHIDCDLFSSTVEILDGLAPRMQAGTVVLFDDYFNYPNWENHGFKAWQDYVAKHGVKY
                     DYIGYARQQAAVRVLDPGAIAK"
     gene            1917..4745
                     /locus_tag="PK634_13260"
     CDS             1917..4745
                     /locus_tag="PK634_13260"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOW98744.1"
                     /translation="MKRLAVVAAAGISLALSSLQAPAVMPRDDDGGSGKVAPEDRDHP
                     NPAEVRRIRERIRLQQELRQRQEQPARRRSTAREAELQADIDRLRLAEDDRVLVILVE
                     FAGSNTFSWTAGTSTWDPLGRCERTEYDGVNFGNTNASAFFAAKYGYSGVSNFTYVGP
                     LHNEIPRPRSADDESGEGIWTEDFSPAFFSNIIFGAGWNFSYLRDDGSPVNADFTGKS
                     VRDYYLDFSGGAYEIVGEVVGWVQVTNSTWWYGADAVPGRRSGANSAASNGGVPGTGN
                     ARQLVIDALEAVKAAYPAFDWAKFDTDSDGVINRLWIIHAGHGEEDSQILLNRTSYGE
                     GALWSHSWSLSAPYEIVPGVSAYSYIMMPENSGIGVLAHEYAHNLGAIDLYTYGDGDT
                     SAGFWTLMADDWVGFPLGFQPPAMDPLHLDQWGWLNPLVINDPSQEYTVTLGQPSDFP
                     GGAEVYRAVKIELPDMVEPLPVAPIGQFQWWGGDEYDASSLMIQSAPLAIPAGGASLV
                     YDTAYDTEAGYDFFRIYVSTNGRVSWTEIAAYDGTSAGYPAYRTLTNNLAAYANRSIN
                     LAFQYWTDSSVLGSGVFLDEVRVVSGVSTLLYDNAESDSGYWQYTAPWTRTGNTKSTP
                     HDYYLQWRNTSGSGGYDSGLGSTNWRFGPVGSGLLVWYHNTRYADNEIADYLTEGPSF
                     GPKGNMLVVDAHPAPYHDPWWLAQGYTGEQANISGRGLMRDAPFSLSDTPAFWIEPPF
                     IQVATGFAGRAAVSLFSDALGYYPGLEKAAMAGDTSRWFTVQWDSSTVIPCASPYGVK
                     AAGYPGNSNFWHIVQDRVTLGTNVFQQYATNLVVDGTAAGGTGNPGALAPVYGWNVRL
                     LSESGTQATVRIWNGSPVEAMTLRPAGSGALAVSCEPVAGRVLSLQASSNLLPGGAEF
                     LTVTNFNTSSNVVVNMDGPRRAYRIGIE"
     gene            4742..>5458
                     /locus_tag="PK634_13265"
     CDS             4742..>5458
                     /locus_tag="PK634_13265"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOW98745.1"
                     /translation="MKKPGDTRILEDLLASLPHRDHPVRAVWSCAFWTLVTTKYSGLS
                     STLRGEERQHSDHADITVPDAGRLLEKTAGELADFALLEDSVAASIGMAAVNSMIEVD
                     ESKCVERGAVDLLVEKGRGRTIGVVGHFGFVPKLRAAAKKLYVIEQRPRPGDLPDTAA
                     PDVLPSCDVVCLTATSFINHTIERLLPLCEKAYVVLTGPTSPLAPALFHHGIDAICGS
                     RVTDPNAVLRLISQGACFKQV"
BASE COUNT          967 a         1801 c         1748 g          942 t
ORIGIN      
        1 ccgtgacctc ggggatgtcg ctctggcggt agaacagggt cttctcggtg tccagcccgc
       61 aggccaggaa gtccagggcc acgtcgatcg acccctgccg catggcgcgc gggtccgtga
      121 ccgacgtcag ggcgtggtag tcggcgatga agaggaagac ctcgccctct ttctgcagat
      181 ccaggatggg cttcatcatc ccgaagtaat ttcccaggtg cagtttgccc gagggttgta
      241 tgccggacag gattctcatg gttcaatacc ttatgttcgt gcgcggcgag ggtatcggaa
      301 ccgccccccg gcatcaaaac aaaattcagg ccgggccttc ggccgggaca gcaggtttgt
      361 cttgaataaa cgcccgcgac gggcgtccaa tgggtgcgcg gatgcagtgg cgatcctata
      421 acggaggttg tggaaatgaa gaagatcatc gggatggcgg ttctggttct ggccctgtcg
      481 gcggggggcg cgatggcgaa caacggctgg ggcctgtacg gcgcctggtg ggagggcggc
      541 gacgccttcg ggccgggctt caagctgacg ttcgaactgg tgccggcgct gcagttcgac
      601 ttccacctcg cctacttcaa cgacgtgggc gacggcgaca cggacctctc cgtcacgccg
      661 ctcgagttcg gcctggccat gaacattccc gtctcggacc agctgaaatt cacggtcggc
      721 ggcggcccga gctacaactt cgtggacgac ggcgacgccg acgacgagat cggcgggtac
      781 atcgggggca gcctggaata ttcgccgtac aaggacgtcg ccctcttcgt cgaggcccgg
      841 tacatcttcc tcgacgccgg ggacgaggac ctcaacggcc ccggcctgaa cgccggcctg
      901 atggtgacgt ggtaaacccc ccgtgcgcgg aatgacggcg ccacgccctc acggcggcgg
      961 gggagatcgg atagtgtttg actccccggg agggcgtggc tagaagactc ccacacatac
     1021 gggagcttca ccatgctggt taccggattc cgcgtcaaac tgtacctgta ccgggccgtc
     1081 caggccttct accggctgat cgaggtcggc cagatccacc cggtgcgcga gctcaagctg
     1141 cgcgccctcc agcggaccac cgactacatc gagaaacacg ccctcgacgc cgtcgggctc
     1201 gacacgcagc gggatctcat cgactacagc ctgcgcgagg tcagggtccc cgggttctac
     1261 ctggagttcg gcgtgttcac cggcggcacg atccggcata tcgccaagca actgcccgcg
     1321 gggcagctca tccacggctt cgacagcttc gaggggctac ccgaggcgtg gagcgggttc
     1381 gacctcggca aggacacttt caacctcaag ggcaagctgc cgaaggttcc ccccaacgtc
     1441 gtcctgcaca agggctggtt caaggacacc atcccggcct ggctgaagga gaacagcggc
     1501 aaaatcgcct tcctgcacat cgactgcgac ctgttctcct ccacggtgga gattctcgac
     1561 ggcctcgccc cccgcatgca ggcggggacg gtcgtgttgt tcgacgacta tttcaactat
     1621 cccaactggg agaatcacgg gttcaaggcc tggcaggact acgtggcaaa gcacggcgtg
     1681 aagtacgatt acatcggtta cgcccgccag caggccgccg tgcgcgtcct ggatccgggc
     1741 gcgatcgcga agtgagcttc cgccgtcgcg ctctcccggc tgaacttcgg gaggccgtgc
     1801 ggactattac tctggacgct tttcccgccg ggaaggataa tgccggcggt tgagaatgaa
     1861 tgccgccggt caacgctacc tgttcctgtt ccacttggaa tctcaaggag cccacgatga
     1921 agagactcgc tgtcgtcgcc gccgcgggga taagtcttgc cctttcgagc ctgcaagcac
     1981 ccgcggtcat gccgcgggat gacgacggcg ggagcggcaa ggtcgccccc gaggatcgcg
     2041 accaccccaa cccggccgaa gttcggcgta tacgcgagcg tatccggctg cagcaggagc
     2101 ttcggcagag gcaagagcag ccggcgcgcc ggcggtcgac cgcccgcgag gcggaactgc
     2161 aggcggatat cgaccggttg aggttggcgg aggatgatcg agtcctggtg atcctggtcg
     2221 agttcgcagg gtccaacacc ttctcttgga ccgcgggcac gtccacgtgg gatccgctcg
     2281 gccgctgcga gcgaacggag tacgacggcg tcaacttcgg caacacgaat gcatccgcct
     2341 tcttcgccgc caagtacggc tacagcggcg tcagcaattt cacctacgtc gggcccctcc
     2401 acaacgagat cccgcggccg cgctccgccg acgacgaatc gggcgaaggc atctggaccg
     2461 aggatttctc gcccgccttc ttcagcaata tcatcttcgg agcgggctgg aacttctcct
     2521 acctgcggga cgacggttcc ccggtgaacg cggatttcac gggaaaatcc gtccgggatt
     2581 actacctcga cttctccggc ggcgcctacg agatcgtcgg cgaggtcgtc ggctgggtcc
     2641 aggtcaccaa ttccacgtgg tggtatgggg cggacgccgt gccgggccga cggtccggcg
     2701 ccaatagcgc ggccagcaac ggcggcgtac ccggcacggg caatgcccgg cagttggtca
     2761 tagatgcgct ggaagccgtc aaggccgcgt acccggcctt cgattgggcc aagttcgaca
     2821 ctgatagtga cggcgtgata aaccggttgt ggatcatcca cgccggccac ggcgaagagg
     2881 acagccagat cctgctcaac cggacctcct acggcgaggg cgccctctgg tcccattcgt
     2941 ggtcgctctc cgcgccctac gaaattgtgc ccggcgtctc cgcctacagt tacatcatga
     3001 tgccggaaaa cagcgggatc ggcgttctgg cccatgaata cgcgcacaac ctgggggcca
     3061 tcgacctgta cacctacggc gacggcgaca catcggcggg tttctggacg ttgatggcgg
     3121 acgactgggt cgggtttccg ctcggcttcc aaccgccggc catggacccc ctccacctcg
     3181 accagtgggg ctggctgaac ccgctggtga tcaacgatcc ctcgcaggag tacacggtta
     3241 cacttgggca acccagcgac ttcccgggcg gcgcggaagt ctaccgcgcg gtcaagatcg
     3301 agttgcccga catggtcgag ccgctgccgg tggcgccgat cgggcaattc cagtggtggg
     3361 gcggcgacga gtacgacgcc agcagtctca tgatccagtc cgcccccttg gccatcccgg
     3421 ccggcggggc ctccctggtc tatgacaccg cgtacgatac cgaggcgggc tatgacttct
     3481 tccgcatcta cgtctccacc aacggccgcg tatcgtggac ggagatcgcc gcctacgacg
     3541 ggaccagcgc gggctacccc gcctaccgga cgctgaccaa caacctcgcg gcctacgcca
     3601 accgcagcat caacctggcc ttccagtatt ggacggactc ttccgtcctg ggcagcgggg
     3661 tcttcctgga cgaggtccgc gtggtgtcgg gcgtgtccac tctcctgtat gacaacgccg
     3721 agagcgattc cggctactgg cagtacacgg ccccctggac gcgcaccggg aacacgaaaa
     3781 gcacgcccca cgattattac ctgcaatgga gaaacacctc gggttccggc ggctatgaca
     3841 gtggtctggg cagcaccaat tggcgcttcg gccccgtcgg ttccgggctg ctcgtctggt
     3901 atcacaacac aaggtacgcc gacaacgaga tcgccgatta cctgaccgag ggcccgtcgt
     3961 tcggcccgaa gggcaatatg ctcgtggtcg acgcccatcc cgccccgtat cacgatccct
     4021 ggtggctggc acaaggctac accggcgagc aggccaacat ctcgggccgc ggcctgatgc
     4081 gagacgcccc gttctccctc tccgacacgc ccgctttttg gatagagccg ccgttcatcc
     4141 aagtcgccac cgggtttgcg ggccgggccg cggtttcact gttcagcgac gccctcggct
     4201 actatcccgg gctggagaag gccgccatgg cgggcgatac cagccgatgg ttcacggtcc
     4261 aatgggacag cagcaccgtg atcccctgcg ccagcccata cggcgtgaag gcggccgggt
     4321 atcccggcaa ttccaacttc tggcacatcg tccaggaccg cgtcaccctg ggcacgaacg
     4381 tgttccaaca gtacgccacg aatctcgtgg tcgacgggac ggccgccggc ggcacgggca
     4441 atcccggggc cctcgccccc gtctatgggt ggaatgtccg tctgttgagc gaatccggca
     4501 cccaagccac cgtgcggatc tggaacggca gcccggtgga ggccatgacc ctgcgcccgg
     4561 ccggaagcgg cgcgctggct gtttcctgcg aaccggtggc cgggcgggtt ctctctctgc
     4621 aggcttcctc caaccttctg cccggcggag cggagttcct tacggtcacc aatttcaaca
     4681 cgtccagcaa tgtggtcgtc aacatggacg ggccccggcg tgcctaccgg atcgggatag
     4741 agtgaagaag ccgggggaca cgcgaatcct ggaggacttg ctggcctccc tgccccaccg
     4801 cgatcatccc gtccgggcgg tatggtcttg cgcgttctgg acgctggtca ccacgaaata
     4861 ctccggcctg tcctccacgc tccggggcga ggaacggcag cacagcgacc atgccgacat
     4921 caccgtgccc gatgcggggc gactcctgga gaaaacggca ggcgagctgg ccgacttcgc
     4981 gctcctcgag gattccgtcg cggcctcgat cggcatggcc gcagtcaact cgatgatcga
     5041 ggtggacgag tcgaaatgcg tcgagcgcgg cgcggtggac ctgctggtcg agaagggccg
     5101 ggggcggacg atcggcgtgg tcgggcactt cgggttcgtg ccgaagctgc gcgcggcggc
     5161 gaagaagctc tacgtgatcg agcagcggcc ccggcccggc gacctgccgg acacggcggc
     5221 gccggacgtc ctgccctctt gtgacgtcgt atgcctgacg gcgaccagtt tcatcaacca
     5281 caccatcgaa cgactgctgc cgctctgcga gaaggcgtac gtggtgctga ccggcccaac
     5341 ctccccattg gcgccggccc tgtttcatca cgggatcgac gccatctgcg gctcgcgcgt
     5401 cacagacccg aatgccgtgt tgcgcctgat cagccagggc gcctgcttta aacaggtg
//
LOCUS       DAOOTK010000167         5449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9355_length_5449_cov_3.589915, whole genome shotgun sequence.
ACCESSION   DAOOTK010000167 DAOOTK010000000
VERSION     DAOOTK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9355_length_5449_cov_3.589915"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK634_13270"
     CDS             complement(<1..993)
                     /locus_tag="PK634_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98746.1"
                     /translation="MSPRKKSTASTSDIVIFIAVLIAIVVVGWLFWKGVQRARHGRET
                     SAIAPDQLPPGARVISGAKRAAVSPVRPLPPGAPRPTVKMDFSKPAPALPTAPTSAPP
                     AAAEIPLEMETGALPRALSAPPAPAIPDSAAGWQTARLAPLADTCIQAHLGTSPDRNG
                     GRLGELPVKGNESFFLARYDLSAIRGWTIRRASWHARVRSGQPHTLGFTLVRAPWEEG
                     SHTLSDSPSGGATFRWAHFNRNPWRADRPPFTHLLRGQPGYLFSVARPLAPPSADNPW
                     IEVPVNPILLQAMAAGLADSLAVSDEKGQLAGAFAVASREMPDEAHFIEVEGAPL"
     gene            complement(1092..1580)
                     /locus_tag="PK634_13275"
     CDS             complement(1092..1580)
                     /locus_tag="PK634_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98747.1"
                     /translation="MKREAVIRGAAAGLFVLGILLAARAAWQTEPVAAQIRAKLADLE
                     QMRSLQAGSAREEQAVAFFEGMADHRPAPLRELAGRLFPGFPSAVQTGEDRPAASGWT
                     IRAAEISLDPVSFADISRLLLELRGDGTRPPWQLTDCQIKASEQGAGQGRVVLGVQAL
                     EK"
     gene            complement(1577..3016)
                     /locus_tag="PK634_13280"
     CDS             complement(1577..3016)
                     /locus_tag="PK634_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98748.1"
                     /translation="MSGPILPARVCLAADLFAGGLRVGRSECARRGLRHEAVLDLAVE
                     DPAPVKAKVAKLEARGGCVWAAALPIHESLTRWLKAPLASEAKARRVLPSLLDIQLPF
                     SIEECVHLFPWIRPSGRGEVEALAVAARTSDLRRRLESLAALGISPVVLDHEGLALWS
                     QSLREEPPRGGGWRVVAALYPDHLALVLGQTDGREPGESRFAGAHSLRPVEALWQGAG
                     NASAEPAVQRVIGRVRQILQAQAASSSIEWLWTGPGAADAALTEDLQHHLGALGSFAF
                     HSHARPESFLVRALATRALRGGAWPCNFRIGSLAHPTVERHLVRRSRGASAALAAAGL
                     VLCGLGLAWPSVLEARKNGVQSELESLAGRLAPGLRLPYGQELRSVRQALQDRAALYR
                     PFLDAFEPSAAELLLEVLQTARAAGLTLDSLGFSGDSVTARGAGADWDRCEQLAGPFR
                     ARGLRVVLERQDAGLDEKVHFTLKGGNAP"
     gene            complement(3013..4278)
                     /locus_tag="PK634_13285"
     CDS             complement(3013..4278)
                     /locus_tag="PK634_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOW98749.1"
                     /translation="MAGIEMASRDPYAGTDVHRFHATRRRLKKITLVLLMIGLISLVI
                     GAGLLLYGLLIARLSARGRMVIMAEIYLGIGGFALLARILLEAVRDRHFRHKRPGAGR
                     EGMALVLVLLVLALVSALVIEAQVSSRAALRREREALLRTRLELAAADAARLALQRLA
                     DDPDLAVDSTNEEWAASSEVMDPAGLTTWTWVEDENRFFDLNNLALPARPSWRPAAEI
                     VMDLLTLCGDFEPVGKADALGDWLDDNAEGAWETGFYRELKPPYAAANRILLSWPELL
                     LVRGFSRDLFARKPRYGLRGAFQADLVDQVTVIPAAREAPVPVNLNTAGEDVLRGVLG
                     IQRDEMVRLILTRRARAPLRSVETALVMLDKDARAAVAPYLDVRSHFFRVDVRADAEG
                     QAVRLRVIARRLKDGRIEVVQWCPGGETG"
     gene            complement(4329..4979)
                     /locus_tag="PK634_13290"
     CDS             complement(4329..4979)
                     /locus_tag="PK634_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOW98750.1"
                     /translation="MSNPFQRSRGAFSLTELLVLVAIAGILAALLVPALSRSRARARQ
                     LRCMGNVRQVAQGLLVVASGEMPRKLPYATDFGSLGGKTGRQAALGGRALAEERPLHQ
                     VLPDPEVFRCPADRGDAVLGVDSVFDAVGSSYLYPVKSVDGIAAAADEPVTGFPFPER
                     KIIVFEPPLLLDRPLRDRRTGWHDARRVSVAGFLDGHAQMVESAGYTNGDPAHAYY"
     gene            complement(5019..>5449)
                     /locus_tag="PK634_13295"
     CDS             complement(5019..>5449)
                     /locus_tag="PK634_13295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98751.1"
                     /translation="AVDRANWRPWLSLGHLHRTRAFWALDPEDKAREAALAFECYEGA
                     AARNPLDMMVRFGIARTYSAQGQPEQALEILRDINRMDRNNMFFMPQLGLQLRNMGRD
                     EEALAVFREAANRWSNEMIAINIRLLERRLAARQAAPLSP"
BASE COUNT          855 a         1920 c         1955 g          719 t
ORIGIN      
        1 cagcggagcg ccttcgacct cgatgaagtg cgcctcgtcc ggcatttccc ggctggcgac
       61 ggcgaacgcg ccggcgagct gtcctttctc gtcgctgacg gcgaggctgt ccgccaggcc
      121 cgcggccatc gcctggagca ggatcggatt gaccggaact tcgatccatg gattgtcggc
      181 cgagggcggc gcgagcggcc gggcgacgga aaagaggtag ccgggctggc cccggagcag
      241 gtgggtaaac ggaggccggt ccgcccgcca tgggttgcgg ttgaaatgcg cccaccggaa
      301 cgtcgcgccg cccgagggcg aatccgacag cgtgtgcgag ccttcctccc acggggcgcg
      361 caccagggtg aacccgagcg tatggggctg gccgctccgc acccgcgcat gccacgaggc
      421 ccgccggatc gtccagccac ggatcgcgga gaggtcatac cgggccagga agaagctctc
      481 gttgcccttc acgggcagtt cgcccaggcg gccgccgttc cggtcgggcg aggtccccag
      541 gtgggcctgg atacaggtat ccgccagcgg cgccagccgc gcggtctgcc aaccggcggc
      601 tgaatccggg atcgcgggcg ccggcggggc cgacagcgcg cgcggcagcg cgccggtctc
      661 catttcgagg gggatctccg ccgcggcggg cggcgcggat gtcggggcgg tcgggagcgc
      721 cggcgccggt tttgaaaaat ccatcttcac ggtcgggcgc ggcgcgccgg gaggaagcgg
      781 ccgaaccggc gatacggcgg ccctcttggc gccggaaatc acccgggcgc cgggcggcaa
      841 ctggtccggg gcaatggccg atgtctcccg gccgtggcgg gcgcgctgca cccccttcca
      901 gaacagccac cctaccacca cgatcgcgat cagcacggca atgaagatca cgatgtcgga
      961 ggtcgaggcc gtgcttttct tgcgggggga catggaggag ggttcagggg tcaggggtgg
     1021 aaaaaaacct ggaaactacg gctccatatt cggctttctg cattccgttg ttcacaaacc
     1081 ttcgcactct gtcatttctc cagcgcctgc acgcccagaa cgacgcggcc ctgcccggcg
     1141 ccctgctcgg aggccttgat ctggcagtcg gtcaactgcc agggcggacg ggttccgtcg
     1201 ccgcgcagtt caagaagcaa gcggctgata tccgcgaacg agaccgggtc gaggctgatt
     1261 tccgcggcgc ggatcgtcca gcccgacgcc gcaggccggt cctcgccggt ctgcacggcc
     1321 gaagggaacc cgggaaacaa gcggccggcc agctcgcgca gcggcgcggg ccgatgatcc
     1381 gccatgccct cgaagaaagc gacggcctgc tcctcgcggg cgctgcccgc ctgcagcgaa
     1441 cgcatctgct cgagatcggc cagcttggcg cggatctgcg cggcgaccgg ttccgtttgc
     1501 cacgccgcgc gggccgccag cagaataccc agcacaaaca ggcccgccgc cgcgccgcgg
     1561 atgaccgctt cgcgcttcac ggggcgttgc ctcccttgag ggtgaagtgc accttctcat
     1621 ccaacccggc gtcctgacgc tcgaggacca cgcgcaggcc gcgcgcgcgg aaaggcccgg
     1681 ccaactgctc gcaccggtcc cagtccgcgc cggcgccgcg ggccgtgacg ctgtccccgc
     1741 tgaaccccag cgaatccagc gtgagcccgg cggcccgggc ggtctgcagg acttcgagca
     1801 ggagttccgc cgcggacggc tcgaaggcgt cgaggaaagg ccggtagagg gcggcccgat
     1861 cctgcaaggc ctggcggacg ctgcgcagtt cctgcccata gggcaggcgc agcccgggcg
     1921 ccagccggcc ggcgagggat tccagctcgg actggacccc gttcttgcgg gcttcgagca
     1981 cggagggcca ggccaggccg agtccgcaga ggacgaggcc cgcggccgct agcgccgccg
     2041 aggcgccccg ggatcgacgg acgaggtggc gttccacggt gggatgggcc agggatccga
     2101 tccggaaatt gcacggccac gcgcctcccc gcagcgcgcg cgtcgccagg gcgcgcacga
     2161 ggaaggactc cggccgggca tgggagtgga acgcgaagct cccgagcgcc ccgagatggt
     2221 gctgcaggtc ctcggtcagg gcggcatccg ccgcgcccgg cccggtccag agccactcga
     2281 tggacgacga ggccgcctgc gcttggagta tctggcgcac gcggccgatc acgcgctgca
     2341 cggcgggctc cgccgaggcg ttcccggccc cctgccacaa ggcctcgacc gggcgcaggc
     2401 tgtgcgcgcc ggcaaaccgg ctttccccgg gctcccggcc gtccgtctgg ccgaggacga
     2461 gggcgaggtg atcggggtag agcgcggcca cgacgcgcca gcccccgccc cggggcggct
     2521 cctcccggag gctctggctc cacagcgcca gcccctcgtg atcgagcacc accggcgaga
     2581 tgccgagggc cgcgagcgat tccaggcggc gccgcagatc cgacgtgcgg gccgccaccg
     2641 cgagggcctc gacctcgccc cgcccggaag gccggatcca cgggaagaga tgcacgcact
     2701 cctcgatgga gaacgggagc tggatgtcga gcagcgacgg caggactctt cgcgccttgg
     2761 cttccgacgc cagcggcgcc ttgagccatc gcgtgaggct ctcgtggatc ggcagcgcgg
     2821 ccgcccagac gcatccgccg cgcgcctcca gtttggctac cttcgccttg accggggcgg
     2881 gatcctccac cgcgagatcg agcacggcct cgtgacgcaa cccgcgccgg gcgcactcgg
     2941 atcggccgac gcggaggccg cccgcaaaga gatccgcggc caggcagacg cgcgcaggca
     3001 ggatcgggcc gctcatcccg tctcccctcc cgggcaccat tgcacgactt cgatgcgccc
     3061 gtccttcagc cggcgcgcga tgacccgaag ccggacggcc tggccctccg cgtccgcgcg
     3121 cacatccacc ctgaagaagt gactccggac atcgagatag ggcgccacgg cggcccgcgc
     3181 atccttgtcc agcatcacca gggccgtttc gacggaccgc aagggcgccc gggcccgccg
     3241 ggtcaggatc aggcggacca tttcgtcccg ctggatcccg agcacgcccc ggagcacatc
     3301 ctcgcccgcc gtgttgagat tgacgggcac cggggcctcg cgcgcggcgg ggatgacggt
     3361 cacctggtcc accaggtcgg cctggaaagc cccgcgaagc ccgtagcggg gtttccgggc
     3421 gaagaggtcg cgggagaatc cgcgcacgag gagcagttcg ggccacgaca ggagtatccg
     3481 gttggcggcg gcgtagggcg gcttgagttc ccggtagaac ccggtttccc acgccccctc
     3541 ggcattgtcg tcaagccagt ctcccagggc gtcggccttg ccgaccggct cgaaatcgcc
     3601 gcagagggtc agcaggtcca tgacgatttc ggcggccggc cgccacgagg ggcgggccgg
     3661 gagggcgagg ttgttgaggt cgaagaagcg gttttcgtcc tccacccagg tccaggtcgt
     3721 caggccggcg ggatccatga cctccgagga ggccgcccac tcctcgttgg tcgaatccac
     3781 ggccaggtcg ggatcgtcgg ccaggcgctg gagcgccagg cgggcggcgt cggcggccgc
     3841 cagttccagc cgggtccgga gcaaggcctc ccgctcgcgc cgcagcgccg cgcgcgagga
     3901 aacctgggcc tcgatcacga gcgcggagac gagggcgagg accagcagga ccaggacgag
     3961 cgccatgccc tcccggcccg cccccggccg cttgtgccgg aaatggcggt cgcgcaccgc
     4021 ttccaggaga atcctcgcca ggagcgcgaa gcctccgatc cccaggtaga tctccgccat
     4081 gatcaccatg cggccgcgcg ccgacagccg ggcgatgagc aggccgtaga gcaggaggcc
     4141 ggcgccgatg accaggctga tcagtccgat catcaggagg accagcgtga tcttcttcag
     4201 acgccgccgg gtggcgtgaa aacgatgcac atccgtcccg gcatatggat ccctggaggc
     4261 catctcgatc cccgccatga tggagaaagc gcggctccgg cgccatgaaa aaaaagagcc
     4321 ggccggactc aataatacgc gtgggccggg tcgccgttcg tgtagccggc cgactccacc
     4381 atctgcgcgt gcccgtccag gaacccggcc acggagaccc tccgcgcgtc gtgccagccg
     4441 gtgcgccggt cgcggagggg ccggtccagg agcagcgggg gctcgaacac gatgatcttg
     4501 cgttcgggga acggaaagcc ggtgaccggt tcatccgcgg ccgccgcgat gccgtccacg
     4561 ctcttcacgg gatagagata gctggagccg acggcatcga agacgctatc gacgccgagc
     4621 accgcgtccc cccggtccgc cgggcaccgg aacacttccg gatcgggcag gacctggtgc
     4681 agggggcgtt cctcggcgag ggcgcgtccg cccagggccg cctgccgtcc cgtctttccg
     4741 cccaggctac cgaagtccgt tgcgtacgga agcttccggg gcatctcgcc cgaggcgacc
     4801 acgaggaggc cctgcgcgac ctgccggacg tttcccatgc agcgcagctg gcgggcccgc
     4861 gccctcgagc gggagagggc cggcacgagc agcgcggcca ggatccccgc gatggccacc
     4921 aggacgagca actcggtcag ggaaaacgcg ccgcgcgaac gctggaacgg gttggacatg
     4981 gggcaatata ggatcgcgcc ggccgatccg caagggcgtc agggcgacaa gggggccgcc
     5041 tggcgcgcgg ccaaccggcg ctcgaggagc cggatgttga tcgcgatcat ctcgttggac
     5101 cagcggttgg ccgcctcgcg gaataccgcc agcgcctcct cgtcccggcc catgttccgc
     5161 agctggaggc ccaactgggg catgaagaac atgttgttgc ggtccatgcg attgatgtcc
     5221 cggaggattt cgagcgcctg ttccggttgg ccctgggcgc tgtaggtccg ggcgatgccg
     5281 aagcggacca tcatgtccag gggattgcgc gcggcggcgc cctcgtagca ttcgaaggcc
     5341 agcgcggcct cgcgcgcctt gtcctccggg tccagggccc agaaggcccg ggtccggtgg
     5401 aggtggccga ggctcaacca cgggcgccag ttggcgcggt ccaccgcgc
//
LOCUS       DAOOTK010000168         5426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9399_length_5426_cov_6.083597, whole genome shotgun sequence.
ACCESSION   DAOOTK010000168 DAOOTK010000000
VERSION     DAOOTK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9399_length_5426_cov_6.083597"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK634_13300"
     CDS             complement(<1..594)
                     /locus_tag="PK634_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98752.1"
                     /translation="MGASLVVVCLLAGGARGAREPSEATTGEEVESFLRQESARLTGQ
                     AEPKRKSVGATPERAPRRILLADIPVKPAGWIPDVDRHAAAAYTEAVREDARQLTLSG
                     RHGQAIAELEKLTVAGEGTDKVLKDVGDYSYRVADYERSGAILKKMLETNPENPEVLC
                     NLSAVLLEQGQGGEALELLERIRLWEVGKPALAAAVLF"
     gene            complement(608..1282)
                     /locus_tag="PK634_13305"
     CDS             complement(608..1282)
                     /locus_tag="PK634_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98753.1"
                     /translation="MGWTNVGSWVTAGTNNALRGQFGSQTFPVPETGSFVATNASSGG
                     AFTGDYGAAGIQLIGFEFMAQDVLPSSALIRWHGASASYFRSFLSYVTETGVWYRLAV
                     TLSDKDAGGWVGGTEEAFQAGLTNVQWVEISITRSGTAAQRYYMDEVFVDGLPEGTRI
                     RFGEATWSPLRTNVAYAVEAAADPKGPWAEAGSFSATNRAQEWRDEGATNEERRVYRL
                     NFRESQ"
     gene            complement(1503..1814)
                     /locus_tag="PK634_13310"
     CDS             complement(1503..1814)
                     /locus_tag="PK634_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOW98754.1"
                     /translation="MIGVESFNGGPEDGDLGQGDGKRCARMLTDLCRAAGIPARVVAG
                     FLQMRAGRQEHIRPHHWTEFHDGYCWRMADCERQRFVVHDEEYLNSRLTGDEETRKEA
                     I"
     gene            complement(2024..3136)
                     /locus_tag="PK634_13315"
     CDS             complement(2024..3136)
                     /locus_tag="PK634_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOW98755.1"
                     /translation="MKARGILLLGLACAARAMEPAEVYRFGTANLNDRLTNRFVLRNE
                     QPNPLGVQSVTPSCECVHVMRWPTNVEAGAAGEIEILFVPDKAGAVDYRLQVKTSSAS
                     MPQLEYAIQGVVTAAPRARVDRDWSLYLGTDEAGALVRDPGRALLVDVRSAQEYERAR
                     IPGSLNIPLYAVKAKGFLRGRKVVLVDEGCGSRVLDEECRKLRGMGFPEVWLWYGGLN
                     AWQRRGGPLEGPGTAGMNRITPIALHDIGFSTDWLVIATGGAKTNQWEGGVIIPFNPA
                     KPGDFAAAVNAAIQERPQVGSVLIASDQGGDYEPVSALAGRINGFVFYLEGGWEAWEA
                     HRRMMGAIRQGHTVVAQGSAPGGGTRVRPGCGGCPR"
     gene            complement(3133..3993)
                     /locus_tag="PK634_13320"
     CDS             complement(3133..3993)
                     /locus_tag="PK634_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOW98756.1"
                     /translation="MSSRVASLVLVMAGSCASVAKDGPAEEPLHLFIRSGFTVQNVGS
                     RVVPAAELWVCAPLEETSTQRLVDWKTEPRSAEQTDALGNHLLHFVFSNVPPYAVKLV
                     SVEATLAMKPSPAPQEADPERWLKAGPLYEYEDEAFERLGPKLPEGSPVQTARALYDW
                     IRGYVRDAGYDGTDRGALYALTQEKGDCTEFATLFAALCRRAGIPARALGGYVVGRNT
                     VLAPASFHNWAEFYADGRWQLADPQRGLFMTGAEHYVTTRVLGESDSPLGSFARFRYL
                     GEGIKAVMNE"
     gene            complement(3990..4700)
                     /locus_tag="PK634_13325"
     CDS             complement(3990..4700)
                     /locus_tag="PK634_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOW98757.1"
                     /translation="MKMKSCLVTILVALAASAPPILAAVIYTETWDATDAGWASGSGD
                     LSVSYAGGFGDPGGSLLGSFSAQEFPDPQTGSFYADSGASGVSFTGDYWSDLGNFYGW
                     TFQFLAADVLPSSLYMRFSDGANTYQANLLSQLGGVGSWSTLTTPALTYGLGGWVGPG
                     GLSGLSNALANVQWVEVRLDRNQEAGQSYYLDNFEHNQDEDPPEPGGGVIPEPGTGIL
                     LLAVLAGRQLVRRRRVTS"
     gene            complement(4815..>5426)
                     /locus_tag="PK634_13330"
     CDS             complement(4815..>5426)
                     /locus_tag="PK634_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98758.1"
                     /translation="YYFEQGTNGCVEISNYAPGEISGNKVVVADAIRFGNGMGDIDRG
                     FGVSGYERELESARYWAQSATQQGMDSGLYDRPGLNDNDDSVGAPTRMADWMDDETDG
                     AFEDRIYLGFHSNAGSGSSRGAMGLYSTGNTPARQALQQAWAQAVSDEIEADMEYLDN
                     GVGFNDDWSDSIASGIPATPPENEYFDEDVGRIGMYLIQVEDE"
BASE COUNT          937 a         2138 c         1398 g          953 t
ORIGIN      
        1 gaagagcacc gccgccgcca gcgccggctt gcccacctcc cacagccgga tccgctccaa
       61 cagctccagc gcctccccgc cttgtccttg ctcgagcaac accgccgaca ggttgcacag
      121 cacctccggg ttctccggat tcgtctccag catctttttc aggatcgccc cggaccgctc
      181 gtaatccgcc acccggtagc tgtaatcccc cacgtccttc agcaccttgt ccgtcccctc
      241 cccggccacg gtcagcttct ccaattccgc aatcgcctgc ccgtgacggc ccgagagcgt
      301 cagttgcctc gcatcctccc tcaccgcctc cgtgtaggcc gccgccgcat gccggtccac
      361 gtcggggatc cagcccgccg gcttcaccgg aatatccgcc agcaggatcc gccgcggcgc
      421 gcgctccggc gtcgctccga ccgacttccg tttcggttct gcctgccccg tcagccgggc
      481 cgactcctgt cggagaaagc tctccacctc ttccccagtc gtcgcttccg agggctcccg
      541 cgcccctcgg gccccgccgg ccagcaggca caccaccacg aggctggcgc ccacccacct
      601 ccttttccta ttggctttca cgaaagttca gccggtatac ccggcgctcc tcgttcgtcg
      661 ccccttcatc cctccactcc tgcgcccggt tggtcgcaga gaaggacccc gcttccgccc
      721 acgggccctt cggatccgcc gccgcttcca ccgcgtaggc cacgttggtg cgcagcggcg
      781 accaggtcgc ttcgccaaac cggatccgcg tcccctccgg caacccgtcc acgaacacct
      841 cgtccatgta ataccgctgc gccgccgtcc ccgaccgcgt aatggatatc tccacccact
      901 gcacgttcgt cagccctgcc tggaacgcct cttccgtccc gcccacccac cccccggcgt
      961 ccttatccga cagcgtaacg gccagccggt accagactcc tgtttctgtt acatacgaca
     1021 gaaagctccg gaaataactg gcggatgctc catgccatcg aatcaaggcc gaggagggca
     1081 gaacatcctg cgccatgaat tcgaaaccga tcaactgaat ccccgccgct ccgtaatccc
     1141 ctgtgaacgc cccgcccgac gaggcattgg tcgctacgaa ggaacccgtt tccggcacgg
     1201 gaaatgtctg cgagccgaat tgaccccgca acgcattatt ggtccccgcc gtcacccatg
     1261 accccacatt cgtccagccc attcgaccgc ctgaaaatgt ttccgtatgg accccggccc
     1321 cccgggcgca cagactcgcg ccggccagga tcagcatcgt ttgtcctatc gtacgcatcc
     1381 ttttcatcaa ggcaggtaca gcagccctac cgctagcagg aacagacccg ccatcaatag
     1441 gaaggccacc agtttgtccg ggctcaagcc ccgtgtatat gaccagcccc tcacgcctct
     1501 gctcatatcg cttcctttcg cgtttcctcg tcccccgtca accggctgtt caggtactcc
     1561 tcgtcatgca ccacaaaccg ctggcgctcg caatccgcca tccgccagca gtatccgtcg
     1621 tgaaactccg tccagtgatg cggccggata tgttcctgcc gcccggccct catctgcagg
     1681 aagcccgcca cgactcgcgc cgggatgccc gcagccctgc acaagtccgt cagcattcgg
     1741 gcgcagcgtt ttccatcccc ctggccaaga tccccgtcct ccggtcctcc gttgaagctt
     1801 tcgacaccga tcatgaacgc gtagagtttc cgaaccgtgg acaggggttc cccgccgcgt
     1861 aacgaccgag cccaagacgc cgcagcgcca ttcgccgccc aaccctcctc ctccgcggcc
     1921 gggcatggcc gttgctccga agccggcagc gggacctctc ccggcgcgtc ccaggatcta
     1981 cgcgatccag actcttgacg ctctccatcc atgcacatcg atttcatcgc ggacatcctc
     2041 cgcatcctgg ccgcactcgg gtccctccgc cgggcgccga gccctgcgcg accacggtat
     2101 ggccctgccg tatcgccccc atcatccgcc ggtgtgcttc ccacgcttcc cagcccccct
     2161 ccagatagaa cacgaaacca ttgatccgcc ccgccagagc cgagacgggc tcgtaatcgc
     2221 cgccctggtc cgaagcgatc aggaccgagc cgacctgcgg ccgctcctgg atcgccgcgt
     2281 tgaccgccgc ggcgaaatcc cccggcttcg cgggattaaa cggaatgatc acgccgccct
     2341 cccactggtt cgtcttcgcc ccgcccgtgg ctatgaccag ccaatccgtg gagaagccga
     2401 tgtcgtgcag cgcgatcggc gtaatgcggt tcatccccgc cgttcccggc ccttccagcg
     2461 gcccgccgcg tcgctgccag gcattcaagc ccccgtacca gagccatacc tccgggaagc
     2521 ccatcccccg caacttccgg cattcctcat ccagcacacg actcccgcac ccctcgtcca
     2581 ccagcacgac cttccgtcct cgcagaaacc ccttggcctt caccgcgtac agcgggatgt
     2641 tcaacgaacc cgggatccgc gcccgctcat actcctgcgc gctgcgcacg tccaccagca
     2701 gcgcgcggcc cggatcccgg acgagcgccc ccgcctcgtc cgtgcccagg tacagcgacc
     2761 agtcccggtc cacccgcgcc cggggcgccg ccgtcaccac gccctggatc gcgtactcga
     2821 gttgcggcat ggaggccgac gaggtcttca cctgcagccg gtagtccacc gcccccgcct
     2881 tgtccggaac aaacaggatc tcgatctcgc ccgccgcccc cgcctccacg ttggtcggcc
     2941 accgcatcac atgcacgcac tcgcaagagg gcgtcaccga ttgcacaccc aggggattcg
     3001 gctgctcgtt gcgaagcacg aaccggttcg tcaaccggtc gttcaggttc gcggttccga
     3061 accggtacac ctcggccggc tccatcgcgc gcgccgcgca cgccaggccc agcagcagga
     3121 tcccgcgcgc cttcattcgt tcatcaccgc cttgatgccc tcgcccagat agcgaaatcg
     3181 cgcaaagctt cccagcgggc tatccgactc ccccagcacg cgcgtcgtca catagtgctc
     3241 cgccccggtc atgaacagcc cccgctgcgg atcggccaac tgccagcgcc cgtcggcata
     3301 aaactccgcc cagttgtgaa aggaggccgg agccaggacc gtattgcggc ccaccacgta
     3361 tccccccagc gcacgcgccg gaatccccgc ccgccggcac aacgccgcga acagcgtcgc
     3421 gaactccgtg cagtcgccct tctcctgcgt caacgcgtac agcgcccccc gatccgttcc
     3481 gtcatagccc gcgtcccgga catagccccg gatccaatcg tatagcgctc gagcggtttg
     3541 caccggactt ccctccggca gtttcggtcc cagccgctca aacgcttcgt cttcatattc
     3601 atacaacggc ccggccttca gccagcgctc ggggtctgcc tcctgcggcg ccggcgaggg
     3661 cttcatcgcc agcgtcgctt ccactgacac cagtttcacc gcgtagggcg gaacgttgct
     3721 gaagacaaag tgaagaaggt gattgcccag ggcatccgtc tgctccgcgg accgcggctc
     3781 cgtcttccaa tccaccagcc gctgggtcga cgtctcctcc agcggcgcgc acacccacaa
     3841 ctccgccgcc ggaacgaccc gggatccgac gttctgcacc gtgaagccgg agcggatgaa
     3901 aagatgcaac ggctcctccg ccggcccgtc cttcgccacc gaagcacagg aaccggccat
     3961 gaccaggacc agcgaggcaa cccgagagct catgacgtta cgcgcctcct ccgtacaagc
     4021 tgacgtccgg ccaggacggc gagcagcagg atccccgtgc ccggttcggg aatcacgcct
     4081 ccgcccggtt cgggcggatc ctcgtcctgg ttatgctcga agttatccag gtagtagctt
     4141 tgccccgcct cctggttgcg atccaggcgc acctccaccc actgcacgtt cgccagcgcg
     4201 ttcgacaacc ccgacagccc gcccggcccc acccagcctc ccagcccata ggtcagcgcc
     4261 ggcgtcgtaa gcgtggacca gctgcccacg cctcccaact ggctcaacag gttcgcctgg
     4321 taggtgttcg ccccgtcgct aaagcgcatg tacagcgacg acggcaacac gtccgccgcc
     4381 aggaactgga acgtccaccc gtagaagttc cccaggtccg accagtaatc ccccgtaaac
     4441 gacacgcccg atgccccgga gtccgcgtag aacgatcccg tctgcgggtc tggaaattcc
     4501 tgcgcggaaa acgagcccag cagcgacccc cccggatcgc cgaacccgcc cgcgtacgag
     4561 accgacaaat cgccgctgcc cgacgcccat cccgcatctg tcgcatccca agtttcggtg
     4621 tagatgacgg cggctaaaat tggcggagca ctggccgcta aagccaccaa tattgtcacc
     4681 aggcaggatt tcatcttcat ttgctcacct ttattctctt tagacacatt cccaaggcca
     4741 tggctatgcg gcatcaggcc cctgcttgag accccgagcg tagaacttca taaatcgagc
     4801 gcttattccc catgctactc atcttccact tgaataagat acataccgat gcggccgaca
     4861 tcctcgtcga agtattcgtt ctccggcggc gtggccggga tgccggacgc gatcgaatcg
     4921 ctccagtcat cgttgaagcc caccccgttg tcgaggtact ccatgtccgc ttcgatctcg
     4981 tcgctaaccg cctgcgccca cgcctgttgc agcgcctgtc tggccggcgt gtttcccgtg
     5041 ctgtataatc ccatggcgcc gcgcgaagag ccgctgccgg cgttcgaatg gaaccccaag
     5101 tagatgcgat cttcaaaggc gccgtccgtt tcgtcgtcca tccagtccgc catccgcgtg
     5161 ggcgcgccga cgctgtcatc gttgtcgttc agacccggcc ggtcatacag tccgctgtcc
     5221 atcccctgtt gggtggcgct ctgcgcccag taacgcgccg actccagctc ccgttcgtag
     5281 ccagacaccc cgaagccgcg gtcgatgtcg cccatgccgt tcccgaaccg aatggcgtcg
     5341 gctacgacca ccttgttccc ggagatctcg cccggagcgt aattggatat ctccacgcac
     5401 ccgttggtgc cctgttcgaa gtaata
//
LOCUS       DAOOTK010000169         5416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9427_length_5416_cov_4.027607, whole genome shotgun sequence.
ACCESSION   DAOOTK010000169 DAOOTK010000000
VERSION     DAOOTK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9427_length_5416_cov_4.027607"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="PK634_13335"
     CDS             complement(<1..1592)
                     /locus_tag="PK634_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW98759.1"
                     /translation="MSRPAFAQLLLLAAFLWGGEMVRRDLWTPDEVRYAYVAGEMRDG
                     GHWLVPHRSGEVYAHKPPLLFWLNNAMAWLTGLPIGRVTSRLPGFLAGLATLWLTARL
                     AERWRGPAAAWPAVLVLCTNYLFWHETGFGRIDGLLLGLTTAAIYLLVRNDDAPGLWR
                     PALAYACMGLAILAKGPVGFIVPAGAYAALRWAAGEGRSLKKSHWLWGPFITLAFPAA
                     WLGLVWWQGAPDGYFRAVLYQQNIARAVGELGHEQPWHYFLTTFPVDLLPWTLLLPAA
                     CLALARDPEAKEFRRRLAGWILFVIAFFTLSTSKRHIYILGAFPAAAILLGGAWDAMA
                     RAPGRWVKAGVFAFLGVYVVGGIGCLAAGLFARLPIPAAVLWPAGLAALGGAAWMIAE
                     LRRRGIALRLFYGIAAAMLLIQWGIGTWVYPAINPLKTPTSLAAEAAAKIAPGHPLLI
                     YQYNGEIFAYYARRPGRVFYSVAEMEAAMRREGRGIAVFLEKDWESVRKDLRVPGTAR
                     PFQSGSKEMIQYDYAAASPAPAG"
     gene            complement(1600..1902)
                     /locus_tag="PK634_13340"
     CDS             complement(1600..1902)
                     /locus_tag="PK634_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOW98760.1"
                     /translation="MSSYISELAQPMVIVGYLGQALFFSRFLVQWIASERLKRSIVPV
                     VFWYLSLGGGALLLAYALWRKDQVIVLGQLVGVFVYVRNLMLIRRHKEPAIPVQPA"
     gene            complement(1899..3476)
                     /locus_tag="PK634_13345"
     CDS             complement(1899..3476)
                     /locus_tag="PK634_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOW98761.1"
                     /translation="MNDAPPASPPGRSIRRLLVFFVLLFLASAVYRPLWEPDEGRYTA
                     AALEMLRAGDWWRPALNPEQPHFSKPPVIYWSLMPGLSWFGRREWAARLPNMAAWIAV
                     LALLAEAGRRFRPGAGGIPALVFASSAFPFVAFSFVTPDILLSLFELIAATGFLFARY
                     RPGKQDSRFWLTLAYAALGAGFLTKGPPVLLPWIAWGITAALLRDRAARQQAAWWPGV
                     IVFLLLGCAWYVAAIAYEPRLLSFFLRGEVVDRIFTSAHGRNPQWYKGFVMYGGILAV
                     GFLPWAPWALRALRGRSDGWFQRLKETEPWSLYFAVWLALGLAVFLLVPSRLPLYILP
                     LAAPVAWLLARSLPESALLRTGTRRALAAWGLAMLLGVVAASHSESKAGSRGLARFLQ
                     QENLLPAEEIVFVESRPYYGLVFYTGAEVERCDLVEPRPLPGAPFLSFETVLEEIQRE
                     QEARLWIVKGTKYESFKKKLVGRAVIVADTGPAYRDFCFVKTETAPGSARAFMPGPPA
                     AGSPVVLGTVAAMKESP"
     gene            complement(3476..4306)
                     /locus_tag="PK634_13350"
     CDS             complement(3476..4306)
                     /locus_tag="PK634_13350"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOW98762.1"
                     /translation="MFLLLGVVAAPGAGVASGPWAGWSQRPKEEKAELLNTLVIGGMG
                     VYGLLFWGYGGHTFQFVDEGWFQQDSIHGGADKLGHAFSTYVGAMSFSAVYENWGYDA
                     HAASRLGALSGFATFFMIEFGDAFSRHGFSLQDLAADAAGALWGYARRECPALRRLVD
                     FRVSYFPSWGSLHGHTSDYISDYSGFTYLLAFQGSGVDALSSTWLKYLELHAGYYTRG
                     YEVQDWCHYGEPHRVVYAGVGLNLSDLVEKTGWRKTATFLEFYQPPWTELSTSYNLDR
                     "
     gene            complement(4437..5297)
                     /locus_tag="PK634_13355"
     CDS             complement(4437..5297)
                     /locus_tag="PK634_13355"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW98763.1"
                     /translation="MHMLIAGAGGVLGRELARQALARGDRVRALVLARRELDGIEHPL
                     LEIREADVTRPETLRGVCEGADRVISCVGITRLKGRMTHEDVDYRGNLNLLREAEKSR
                     VGKFGFISPAGTPEGHREAPLLAAKHRFETALRASAVPWVIFRSGGFFSDLAEMKKMA
                     ARGPLFVIGRGTEKSTPIHVADLAEIMLADMETTWNAVVEAGGPEHLSWLDICRTCFE
                     AQNRPPKIIRVPAGLCRATLAVLRPFSPRRHAMGQLLLFMSTRDVCTPPRGRLKLREY
                     LRSLDGRATA"
BASE COUNT          987 a         1885 c         1841 g          703 t
ORIGIN      
        1 ccggcgggcg ccggcgacgc ggcggcgtag tcgtactgga tcatttcctt gctgcccgac
       61 tggaacggcc gggcggttcc cgggacgcgc aggtctttcc gaaccgattc ccagtccttc
      121 tccaggaaga cggcgatacc gcgcccctcg cggcgcatgg cggcctccat ttcggcgacc
      181 gagtagaaca cgcgtccggg ccgccgggcg tagtaggcga agatctcgcc gttgtactgg
      241 tagatcagca ggggatgccc cggcgcgatt tttgccgccg cctcggcggc gagcgaggtg
      301 ggggtcttga gcgggttgat ggcgggatag acccacgtgc cgatacccca ctggatcagc
      361 agcatggccg cggcgatccc gtagaacagg cggagcgcga tgccgcgccg gcgcagttcg
      421 gcgatcatcc aggccgcgcc gcccagggcc gcaaggcccg cgggccagag aacggcggcc
      481 gggatcggga gccgcgcgaa caagccggcg gccaggcagc cgatgccgcc gacaacataa
      541 acgcccagga aggcgaagac gccggccttg acccaccgcc cgggcgcgcg cgccatggcg
      601 tcccacgcgc cgcccagcag aatcgccgcc gcagggaaag cgcccaggat gtagatgtga
      661 cgcttgctgg tgcttaacgt aaagaaggcg atcacgaaca ggatccagcc cgcgaggcgg
      721 cggcggaatt ccttcgcctc gggatcgcgg gccagcgcaa ggcacgcggc cgggaggagc
      781 agcgtccacg gcagcaggtc cacggggaag gtggtgagga aatagtgcca gggctgctcg
      841 tggcccagtt cgccgacggc gcgcgcgata ttctgctggt acagcaccgc gcggaagtag
      901 ccgtccggcg cgccctgcca ccagaccagg ccgagccagg cggcggggaa cgccagggtg
      961 atgaacggcc cccagagcca atggcttttc ttcaggctcc gcccctcccc ggccgcccag
     1021 cgcagggccg cgtaggcgcc ggcgggcacg atgaacccga cgggcccctt cgccaggatg
     1081 gctaggccca tgcaggcgta ggccagggcc ggccgccaga ggcccggcgc gtcgtcgttg
     1141 cgaaccagca ggtagatcgc cgccgtggtc aggccgagca gcaggccgtc gatccggccg
     1201 aacccggtct cgtgccagaa gaggtaattc gtgcacagca ccagcacggc gggccaggcc
     1261 gccgccggcc cgcgccagcg ttcagcgagc cgcgcggtca gccagagcgt ggctaggccc
     1321 gcgaggaagc cgggcaggcg cgaggtcacg cgcccgatgg gcaggccggt gagccaggcc
     1381 atcgcgttgt tcagccagaa gagcagcggg ggcttgtgcg cgtagacctc gccgctccgg
     1441 tgcgggacca gccagtggcc gccgtcgcgc atctcgcccg cgacgtacgc gtagcgcacc
     1501 tcgtccggcg tccacaggtc gcggcggacc atctcgccgc cccagaggaa ggcggccagc
     1561 agcagcaact gcgcaaaggc ggggcgggac atggccggcc tacgcgggct ggacggggat
     1621 ggccggctct ttgtgccggc ggataagcat gaggtttcgg acataaacaa aaaccccgac
     1681 cagctggccc aggacgatga cctggtcctt tcgccagagc gcataggcca gcagcagcgc
     1741 gccgccgccg agggaaagat accagaacac gaccggcacg atcgaccgct tcagccgctc
     1801 ggaggcgatc cactggacca ggaagcggga gaaaaacagg gcctggccca ggtacccgac
     1861 gatcaccatc ggctgcgcca gttccgaaat ataactactc atgggctctc cttcatggct
     1921 gcaacggttc cgaggacgac cgggctgccc gcggcgggcg gccccggcat gaatgcccgc
     1981 gccgaacccg gcgcggtctc cgttttgacg aagcagaaat cccggtaggc cggccccgtg
     2041 tccgccacga tcaccgcccg cccaacgagt ttcttcttga agctctcgta tttcgttcct
     2101 ttcacgatcc acaagcgagc ctcctgctcg cgctggattt cctccaggac ggtctcgaac
     2161 gacaggaacg gcgcgccggg cagggggcga ggctcgacga gatcgcaccg ctcgacctcc
     2221 gcgccggtgt aaaacacgag cccgtaatag ggccgcgatt cgacgaagac gatttcctcc
     2281 gcgggcagga gattctcctg ctgcaggaac cgggccaggc cgcgggagcc cgccttggat
     2341 tccgaatggc tggctgcgac aacgccgagg agcatcgcca ggccccaggc ggccagcgcc
     2401 cggcgcgtcc ccgtccgaag cagcgcggat tccgggagcg accgggccag cagccaggcc
     2461 acgggggcgg ccaggggaag gatgtagagc ggcaggcgcg aggggaccag caggaagact
     2521 gccaggccga gggccaacca gaccgcaaaa tacagggacc agggctcggt ttctttcagg
     2581 cgctggaacc acccgtccga tcgcccgcgc aacgcgcgca gggcccacgg cgcccagggg
     2641 agaaacccga cggccagaat gccgccgtac atcacgaatc ccttgtacca ctgcggattg
     2701 cgcccatggg cggaggtgaa gatccggtcc acgacctccc cgcgcaggaa gaaggaaagg
     2761 agccggggct cgtacgcgat ggcggccacg taccaggcgc atcccaggag gagaaacacg
     2821 atcacgccgg gccaccacgc cgcctgctgc cgggccgccc ggtcgcgcag gagcgccgcg
     2881 gtgatccccc acgcgatcca gggcagcagg acgggcgggc ccttggtcag gaaccccgcg
     2941 cccagcgccg cgtaggcgag cgtcagccag aaccgggaat cctgcttgcc cggccggtag
     3001 cgcgcgaaca ggaatcccgt cgcggcgatc aactcgaaga gcgacaggag gatatcgggc
     3061 gtcacgaagg aaaacgcgac gaaagggaac gccgaactgg cgaagaccag cgcggggatc
     3121 ccgccggctc cgggacggaa gcggcgcccg gcctcggcca gcagcgcaag gaccgcgatc
     3181 cacgccgcca tgttgggcag gcgcgcggcc cattcccggc gcccgaacca cgagaggccc
     3241 ggcatcaggg accagtaaat gacgggaggc tttgaaaaat gcggctgctc cgggttgagt
     3301 gccggccgcc accagtcgcc ggcgcgcagc atctccagcg cggcggccgt gtaccgccct
     3361 tcgtccggct cccacagcgg gcggtagacg gcggaggcca ggaacagcag gacaaaaaaa
     3421 acgagcaggc ggcgaatcga ccgacccggc ggggaggctg gaggcgcgtc gttcatcacc
     3481 ggtccaggtt gtaactcgtt gaaagttccg tccacggcgg ctggtagaat tccaggaagg
     3541 tcgcggtctt gcgccatccg gtcttctcga ccaggtccga caggttcaag ccgacgcccg
     3601 cgtacacgac ccggtgcggc tcgccgtagt ggcaccagtc ctggacctcg tagcctcgcg
     3661 tgtaatagcc ggcgtggagc tcgagatact tcagccaggt cgacgacagg gcgtccaccc
     3721 cgctgccctg gaaggccagg agatacgtga agccggagta atcgctgatg tagtccgagg
     3781 tatggccgtg gagggaaccc cacgacggga agtacgagac ccggaaatcc accagtcggc
     3841 gcagcgccgg acactcccgg cgggcgtacc cccacagcgc gcccgccgcg tccgccgcga
     3901 ggtcctgcag ggaaaagccg tggcggctga aggcgtcccc gaactcgatc atgaaaaacg
     3961 tggcgaagcc cgacagggcg cccaggcgcg acgccgcgtg ggcgtcgtag ccccaattct
     4021 catacaccgc gctgaagctc atcgcgccca cgtacgtgct gaacgcgtgg cccagcttgt
     4081 cggccccgcc gtggatgctg tcctgctgaa accagccctc gtcgacgaat tgaaacgtgt
     4141 gcccgccgta tccccagaac agcaggccgt acacgcccat cccgccgatg acgagcgtgt
     4201 tgagcagttc cgctttctct tccttgggcc gctgcgacca ccccgcccag ggcccgctcg
     4261 cgacgcccgc tccgggcgcg gcgaccaccc ccaggagcag aaacacgaag cccctcttcc
     4321 aaggccgcaa gttgtgacga ggaaacgtca tgagtgcagg tcgttcaacg ccgatactca
     4381 tgaccagcgt aggtcggcgc gaccaactcc tgccatccaa caatccttaa gatttcttaa
     4441 gccgtggccc ggccgtccaa gcttcgcagg tactcgcgca gcttcaggcg cccgcgcggc
     4501 ggggtgcaga catcgcgcgt ggacatgaac agcaacagct ggcccatcgc gtggcgccgc
     4561 ggggaaaaag gccggagcac ggccagcgtc gcccggcaga gccccgccgg cacgcggatg
     4621 atcttcggag gtcggttctg cgcctcgaag caggtccggc agatgtccag ccagctcaag
     4681 tgttccggcc cgccggcctc gacaacagcg ttccaggtcg tctccatatc ggccagcatg
     4741 atctccgcga ggtccgcgac gtggatcggc gtgctcttct ccgtgccgcg gccaatgaca
     4801 aagagcgggc cgcgcgcggc catcttcttc atttcggcca ggtcggagaa gaagcccccg
     4861 gaccggaaga tcacccacgg cacggcggac gcgcgcaggg ccgtttcgaa gcgatgcttg
     4921 gccgccaaca gcggcgcttc gcgatgcccc tcgggcgtgc ccgcgggcga gatgaagccg
     4981 aacttgccga cgcggctctt ctcggcctcg cgcaggaggt tcaggttgcc gcgatagtcc
     5041 acgtcctcgt gcgtcatgcg ccccttgagc cgggtgatgc cgacgcacga gatgacccgg
     5101 tccgcgccct cgcagacgcc gcgcaacgtc tccggccggg tgacatccgc ctcccggatt
     5161 tcgaggagcg gatgctcgat cccgtccagt tcccggcggg caaggaccag cgccctcacc
     5221 cggtccccgc gcgccagggc ctgccgcgcg agttcgcgcc cgaggacccc gcccgccccc
     5281 gcgatcagca tgtgcatggc cgtcctccgt tcagggcagg tactcaatcc gccattccgt
     5341 ggacggcagg gcgagcgtca cggacttcca gtcgcccttc cggtcgccat cctcttcctc
     5401 ggccaggacg ccgcgg
//
LOCUS       DAOOTK010000170         5410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9446_length_5410_cov_5.234360, whole genome shotgun sequence.
ACCESSION   DAOOTK010000170 DAOOTK010000000
VERSION     DAOOTK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_5410_cov_5.234360"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..310
                     /locus_tag="PK634_13360"
                     /pseudo
     CDS             1..310
                     /locus_tag="PK634_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            944..1126
                     /locus_tag="PK634_13365"
     CDS             944..1126
                     /locus_tag="PK634_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98764.1"
                     /translation="MNPEQLWETTMNPEKRKMLKVTLEDGVKADEIFTVLMGDEVEPR
                     RQFIEDNALNVRNLDI"
     gene            1209..3680
                     /gene="gyrA"
                     /locus_tag="PK634_13370"
     CDS             1209..3680
                     /gene="gyrA"
                     /locus_tag="PK634_13370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOW98765.1"
                     /translation="MYDRNDRVDLINIEDEMQRAYIDYSMSVIVGRALPDARDGMKPG
                     NRRILFAMREGSWLHNRPYVKCARVVGDVIGKYHPHGDAAVYDTLVRMAQDFSMRYLL
                     IDGQGNFGSIDGDPPAAYRYTECRLEQLAEEMLADIDKNTVDMQPNYDEKMLEPKVLP
                     SRIPNLLVNGSTGIAVGMATNIPPHNLGEIVDATVHLIDNPDAPVAALMQFVKGPDFP
                     TAGMLCGTTQIKSMYETGRGLLKIRGRASIEEGPQGKESIIIHDLPYTVNKASLIGNL
                     ADLVNDKKIEGISDIRDESDKDGIRVVIEIKRGAIPKVILNNIYQHTQLESTFGAIML
                     AIDRGRPRVMNLREMIQCFVAHRFEVITRRAKFDLEKAEARAHILEGLKIALDHLDAV
                     VKTIRESKDRDGARTRLMERFKLSEIQANAILDMRLYQLTNLERAKIDAEYEEIIKLI
                     NYLRDLLATERKIYGVIKDDLLEMKKLYGDARRTDIVPDEGEIAIEDLIADRGCVITI
                     SHAGYIKRVPVATFKQQRRGGKGVVGMDTKEEDYVEHVFTASTHDYMLFFTAKGRVYW
                     KKVYEIPEGGRATRGKAIVNFLEIGSDEKIAACIPVREFPESQFLVFATANGLVKKTN
                     LAAFGNPRAGGIIAITIEEGDTLVGVRLTNGQNELILVTRQGMSIRFHENQMRDQGRG
                     TVGVYGIKLEKDDRVENSEVVEPNATLMCISENGYGKRTAFDEYPDQHRGGKGVITMK
                     TTERNGLVVGAHAVRESDAFMIITERGQMIRMPVSDVRVISRNTQGVRLINLDEGDKV
                     VSATPVEPEDGDENGAEATPPAPPA"
     gene            complement(3730..4353)
                     /locus_tag="PK634_13375"
     CDS             complement(3730..4353)
                     /locus_tag="PK634_13375"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOW98766.1"
                     /translation="MAHELPKLPYAFDALEPHVDARTMEIHHGKHHAAYVHNLNAALE
                     KHPELQGRSLESLLADLSKVPEDIRTAVRNNGGGHWNHSLFWQWMAPKSPGKPVGAVA
                     KAIDAAFGGFDAFKEQFGKAAVGRFGSGWAWLCVKDGQLCVCSSPNQDNPTMKGVADC
                     VGAPVLGLDVWEHAYYLKYQNRRADYIAAWWNVVNWEKVESLYVAAR"
     gene            4500..>5410
                     /locus_tag="PK634_13380"
     CDS             4500..>5410
                     /locus_tag="PK634_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOW98767.1"
                     /translation="MRVEKDSLGERQVPDEAYYGIHTARSLENFDIAGEPMPLEIVYG
                     LVKIKWATATANAALGMIPADKADAIKAACLRVLKGEFDGEFKVDVFQAGSGTSSNMN
                     VNEVLANIANEALGGRRGDRRPVHPHDDVNLGTSTNNTFPSAVKIALVEKASAMLLSL
                     RRLVEELHGKEREFKDILKTGRTHLQDAVPITLGQEFGAWARALEKDVRRIESARLKL
                     RELGVGGNAIGTGVNTRREFRPILIRALGTFTGEVYEGAENGIEITQFMTDLGEYSSA
                     LKLLALDVGKVCNDLRLLSSGPNTGLD"
BASE COUNT         1055 a         1763 c         1809 g          783 t
ORIGIN      
        1 cgacagcaaa ttccaggcga tcctgccgat tcgcgggaag ctgctgaacg tggagaaggc
       61 gcgactggac agggcgctgc acaacaacga aatccggacg atgatcaccg cgctgggcac
      121 cggtttcggg cgcgaggagt tcaagatcga gaacctgcgc tacaacaagg tcatcatcat
      181 gaccgacgcg gacgtggacg gctcgcatat ccgcacgctc ctgctgacgt ttttcttccg
      241 taagatgccg gcgctgatcg agcgcgggaa cgtcttcatc gcgcagccgc cgctgtacaa
      301 gatcaagcgc aagaaccgcg aggagtacat cgagaacgac gcgcacatga cgcgcatcct
      361 gctcgagctc ggcgtggagg agctgaagct ggccgacgcc aagggcaagg agctgttgag
      421 cggcaagccg ctgtcggacc tgctggatct cctgttcgag atcgagacgc acctcgaccg
      481 cgtccggcgg cgcggcgtgg acgtggcgga gtacctgcag caccgcgacc cgaagacggg
      541 ccagcttccg cagtaccgcg tcacgctgac ggtgggcggg gagcccgagc accggttcgc
      601 gttcacggag tcggagctgc gggccctgcg ggaggaggcc gagaagcggt ccggcgagca
      661 gctcgtgatc ttcggggagg gcggcgagga ggcgaaggcc cggagccagg gcatccggtg
      721 gacggagctc tactcggcgc ccgcgctcgg caagctgctg ctgacgctgg agaagaaggg
      781 gttccaggtg gcgaatctcc tgccgcagga gcaaccgctc ctgatcctca agaacggcga
      841 cgagaaggac ctggcgatct cttcgcttca ggaactgctg aaccgcgtcc gcgagcacgg
      901 cggcaagggc ttgagcatcc agcggttcaa gggcctgggc gaaatgaacc ccgagcagct
      961 ctgggagacc acgatgaacc cggagaagcg caagatgctc aaggtcacgc tcgaagacgg
     1021 cgtgaaggcg gacgagatat tcaccgtgct gatgggcgac gaggtcgagc cgcgccgcca
     1081 gttcatcgag gacaacgcgc tgaacgttcg gaacctggac atttaaaaag ggagccgcgg
     1141 attacgcgga taggaaggct ttttatctat acccgcggcc tccgcgaaat ccgcggccaa
     1201 gaaattctat gtacgaccga aacgacagag tcgacctgat caacatcgaa gacgagatgc
     1261 agcgggcata catcgactac tcgatgtccg tcatcgtcgg ccgcgcgctg cccgacgccc
     1321 gcgacgggat gaagccgggc aaccggcgca tcctcttcgc catgcgcgag ggcagctggc
     1381 tgcacaaccg gccgtacgtc aagtgcgcgc gcgtcgtcgg cgacgtgatc ggcaagtacc
     1441 atccgcacgg cgacgccgcg gtctacgaca cgctcgtgcg catggcgcag gatttctcga
     1501 tgcgctacct gctgatcgac gggcagggca acttcggctc gatcgacggc gacccgcccg
     1561 cggcctaccg gtacacggag tgccgcctgg agcagctcgc cgaggagatg ctggcggaca
     1621 tcgacaagaa cacggtcgac atgcagccga actacgacga gaagatgctc gagccgaagg
     1681 tgctcccctc gcgcatcccg aacctgctcg tcaacggcag cacgggcatc gccgtgggca
     1741 tggcgaccaa catcccgccc cacaacctgg gcgagatcgt ggacgccacg gtccacctga
     1801 tcgacaatcc cgacgcgccg gtcgcggccc tgatgcagtt cgtgaagggg ccggacttcc
     1861 ccacggcggg catgctctgc ggcaccacgc agatcaagtc catgtacgag accggtcgcg
     1921 gcctgctgaa gatccgcggc cgcgcctcga tcgaggaggg gccgcagggc aaggaaagca
     1981 tcatcatcca cgacctgccc tacacggtga acaaggcctc cctgatcggg aacctggccg
     2041 acctggtcaa cgacaagaag atcgagggca tctcggacat ccgggacgag tccgacaagg
     2101 acggcatccg ggtcgtgatc gaaatcaagc ggggcgcgat cccgaaggtc atcctcaaca
     2161 acatctacca gcacacgcag ctcgagtcca cgttcggcgc gatcatgctg gcgatcgacc
     2221 gcggccgccc gcgcgtgatg aacctccggg agatgatcca gtgcttcgtc gcgcaccggt
     2281 tcgaggtcat tacgcggcgc gcgaagttcg acctggagaa ggccgaggcc cgcgcgcaca
     2341 tcctcgaggg cctgaagatc gcgctcgacc acctcgacgc cgtggtcaag accatccgcg
     2401 agtccaagga ccgcgacggc gcgcggacgc gcctgatgga gcggttcaag ctctccgaga
     2461 tccaggcgaa cgccatcctc gacatgcggc tctaccagct caccaacctc gagcgggcga
     2521 agatcgacgc ggagtacgag gagatcatca agctgatcaa ctacctgcgc gacctgctgg
     2581 ccaccgagcg caagatctac ggcgtgatca aggacgacct gctcgagatg aagaagctct
     2641 acggcgacgc ccgccggacg gacatcgtgc ccgacgaggg cgagatcgcc atcgaggacc
     2701 tcatcgccga ccgcggctgc gtgatcacca tcagccacgc gggctacatc aagcgcgtgc
     2761 cggtggcgac gttcaagcag cagcgccgcg gcggcaaggg cgtcgtcggc atggacacca
     2821 aggaggagga ctacgtcgag cacgtcttca cggcctccac ccacgactac atgctcttct
     2881 tcaccgccaa ggggcgggtg tactggaaga aggtctacga gatccccgag ggcgggcgcg
     2941 cgacgcgcgg caaggcgatc gtcaatttcc tcgagatcgg gtccgacgag aagatcgccg
     3001 cgtgcatccc tgtgcgcgaa ttcccggaga gccagttcct cgtgttcgcc acggccaacg
     3061 gcctggtgaa gaagaccaac ctcgccgcgt tcggcaaccc gcgcgcgggc ggcatcatcg
     3121 ccatcaccat cgaggagggc gacacgctgg tcggcgtgcg gctgacgaac gggcagaacg
     3181 agctgatcct cgtcacccgg cagggcatga gcatccggtt ccacgagaac cagatgcgcg
     3241 accaggggcg cggcaccgtc ggcgtctacg ggatcaagct ggagaaggac gaccgcgtcg
     3301 agaacagcga ggtcgtcgag ccgaacgcca cgctgatgtg catctcggag aacggctacg
     3361 gcaagcgcac ggcgttcgac gagtacccgg accagcaccg cggcggcaag ggcgtgatca
     3421 cgatgaagac gaccgagcgc aacggcctgg tcgtcggcgc ccatgccgtg cgggaaagcg
     3481 acgcgttcat gatcatcacc gaacgcgggc agatgatccg catgccggtc agcgacgtcc
     3541 gcgtcatcag ccgcaacacc cagggcgtcc gcctgatcaa cctcgacgag ggcgacaagg
     3601 tcgtgtcggc cacgccggtc gagcccgagg acggggacga gaacggcgcg gaggcgactc
     3661 cgcccgcgcc gccggcctga aaaaaggggc ggctcgcgcc gcccccgtcc aggccccctc
     3721 gcggccgcgt cagcgcgcgg cgacgtagag cgattccacc ttctcccagt tcacgacatt
     3781 ccaccaggcg gcgatgtagt ccgcgcggcg gttctggtat ttcagatagt aggcgtgctc
     3841 ccagacgtcg aggcccagga ccggcgcgcc gacgcagtcg gccacaccct tcatcgtcgg
     3901 gttgtcctgg ttcggcgagg agcaaacgca gagctggccg tccttgacgc acagccacgc
     3961 ccagccggag ccgaagcggc cgacggcggc cttgccgaac tgctccttga aggcgtcgaa
     4021 gccgccaaac gccgcgtcga tcgccttcgc gacggcgccg accggtttgc ccgggctttt
     4081 cggggccatc cactgccaga acaggctgtg gttccagtgc ccgccgccgt tgttgcggac
     4141 ggcggtccgg atgtcctcgg ggaccttcga gaggtcggcc aggagcgact ccaagctccg
     4201 gccctggagc tcggggtgct tctccagcgc cgcgttcagg ttgtggacgt aggccgcgtg
     4261 gtgcttgccg tggtggatct ccatcgtgcg cgcgtccacg tggggctcca gcgcgtcgaa
     4321 ggcgtacggc agtttcggta gttcgtgtgc catggtttca ccctttcgtt ttatcccccg
     4381 cggaacccgc gggagggtaa atatagggca ccccgggcat ttttccagag gccctccgtt
     4441 tgccatttga ccgggggggc gtttccgcta ccatcgcgcg agaacatgga gcggcaccga
     4501 tgagagtgga gaaggattcc ctgggcgagc ggcaggtccc cgacgaggcg tactacggca
     4561 ttcacacggc ccggtcgctg gagaatttcg acatcgccgg cgagccgatg ccgctggaga
     4621 tcgtctacgg gctggtcaag atcaagtggg ccacggccac cgccaacgcc gcgctgggca
     4681 tgatcccggc ggacaaggcc gacgccatca aggccgcgtg cctgcgcgtg ctgaagggcg
     4741 agttcgacgg cgagttcaag gtggacgtgt tccaggccgg ctcgggcacg tcctcgaaca
     4801 tgaacgtcaa cgaggtgctc gccaacatcg ccaacgaggc cctcggcggc cggcgcggcg
     4861 accgccggcc ggtccacccg cacgacgacg tcaacctcgg cacctcgacg aacaacacct
     4921 tcccctccgc cgtcaagatc gcgctggtgg aaaaggcctc cgcgatgctg ttgagcctcc
     4981 ggcgcctggt cgaggaactg cacgggaagg agcgggagtt caaggacatc ctgaagaccg
     5041 gccgcaccca cctgcaggat gccgtgccca tcacgctggg ccaggagttc ggcgcctggg
     5101 cgcgcgcgct ggaaaaggac gtgcgccgca tcgagtcggc ccgcctgaag ctgcgggagc
     5161 tgggcgtggg cggcaacgcg atcggcacgg gggtcaatac ccgccgcgag ttccggccga
     5221 tcctgatccg cgccctgggc acgttcacgg gcgaggtcta cgaaggcgcg gagaacggca
     5281 tcgagatcac gcagttcatg acggatctcg gggaatactc ctccgccctg aagctgctgg
     5341 cgctggacgt cggcaaggtg tgcaacgacc tgcggctcct ctcctccggc ccgaacacgg
     5401 gcctggatga
//
LOCUS       DAOOTK010000171         5407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9450_length_5407_cov_4.500934, whole genome shotgun sequence.
ACCESSION   DAOOTK010000171 DAOOTK010000000
VERSION     DAOOTK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9450_length_5407_cov_4.500934"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK634_13385"
     CDS             <1..233
                     /locus_tag="PK634_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOW98768.1"
                     /translation="PRVAGVCDACGGELYQRADDTEETVLNRLEVFRKQTESLIEYYE
                     KKGVLVRINAGRNRELVQADILAALRKPAAGT"
     gene            230..985
                     /gene="map"
                     /locus_tag="PK634_13390"
     CDS             230..985
                     /gene="map"
                     /locus_tag="PK634_13390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOW98769.1"
                     /translation="MIVIKTPEDLARMRVSGRLAAEVIEELARRVGPGVTTKELDDLA
                     RELMAQRGGRSAFFGYRGYPGQTCISINDEVVHGIPGPRRIRLGDIVSLDVGVVYEGY
                     VGDTATTVMVGVSDPEVVQLVSTGRRALEAAIGMAVAGGRLSDISHAIESTARAAGYS
                     VVRDFVGHGVGKAMHEDPQIPNFGPPGRGPRLKPGMTLAIEPMINRGGHEVEVMDDGW
                     TVRTRDRSLSVHFEHTIAVGEGAAEVLTWPGPK"
     gene            967..1194
                     /locus_tag="PK634_13395"
     CDS             967..1194
                     /locus_tag="PK634_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOW98770.1"
                     /translation="MAGPEIIRLDARVVSVITNAVFRVELANGHGLVAYAARAEKARA
                     EAVSPGDRVRVELSPCDMSRGRLVFREEENG"
     gene            1191..1304
                     /gene="rpmJ"
                     /locus_tag="PK634_13400"
     CDS             1191..1304
                     /gene="rpmJ"
                     /locus_tag="PK634_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOW98771.1"
                     /translation="MKVRSSVKRICERCKVVRRKRVVRVICNNPRHKQRQG"
     gene            1316..1717
                     /gene="rpsM"
                     /locus_tag="PK634_13405"
     CDS             1316..1717
                     /gene="rpsM"
                     /locus_tag="PK634_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOW98772.1"
                     /translation="MPRVLGIEIPGKKRVEYALRYIYGIGLTRARQVVAQAGINPAKK
                     ADELTDEEITGISNALQGYNLEGDLRREVASNIRRLIGIGCYRGSRHRKGLPVRGQRT
                     STNARTRKGPRKTVGAVRGKEARSEVKSDKE"
     gene            1738..2247
                     /gene="rpsK"
                     /locus_tag="PK634_13410"
     CDS             1738..2247
                     /gene="rpsK"
                     /locus_tag="PK634_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOW98773.1"
                     /translation="MADEKEKQEVKAPPAEAAVAEAAAPGAVPAEGAAPAAEGEAAKP
                     VKRKAVKGARTVPVGVVYVRATFNNTMVTITDMRGAVVCWSTAGRCGFKGSRRSTAYA
                     ATVVAQEAARQAIGFGMHEVEVRIQGPGAGRESAIRAIQSAGLTITVIKDITPVPHNG
                     CRPPKRRRV"
     gene            2278..2904
                     /gene="rpsD"
                     /locus_tag="PK634_13415"
     CDS             2278..2904
                     /gene="rpsD"
                     /locus_tag="PK634_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOW98774.1"
                     /translation="MGRYTGPVCRMCRREGMKLFLKGDRCFMAKCPIETGRSAPGMHG
                     QRRSKLSDYGVQLREKQRLRRMYGMQEGQFHLFFTRALRKRGVTGETLLQSLECRLDN
                     VVFRLGFAPSRRAARQFVRHGHIQVNGNKVDIPSLQLKAGDIVQVRDRAQSREYANRH
                     MEVAESRGIVGWLALDRANARGEVLHAPTRDEIAPVVREQLIVELYSK"
     gene            2960..3982
                     /locus_tag="PK634_13420"
     CDS             2960..3982
                     /locus_tag="PK634_13420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOW98775.1"
                     /translation="MPVRLARFEMPKRIVKDEAAAAPNYGKFIAEPFEAGYGRTLGNS
                     LRRVLLSSIEGAAIASVKIEGVQHEFSTLPGMVEDVTDVILNLKQVLLKMYSRAPRKL
                     RISVKGPGEVKAGDIQVDSMVEVLNPDHKIATLDKDGKFDAELEIKIGRGYCPAEWNK
                     KEEQEIGLIPIDSLFSPVRRVKYTVENTRVGRRTDYDRLILEVWTDGRQTPDESLAVS
                     AALLRHHLDVFVSPEHEEIEIAEATPEINPEREELRRRLNISVNEIELSVRAANCLNN
                     ANITTVGQLAQKTEAEMLKYRNFGRKSLNEIKQKLSELGLTLGMSFDADVLKPLEVEV
                     KEEPAE"
     gene            4007..4495
                     /gene="rplQ"
                     /locus_tag="PK634_13425"
     CDS             4007..4495
                     /gene="rplQ"
                     /locus_tag="PK634_13425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOW98776.1"
                     /translation="MRHRKDTLKLGRTAAHRDELLGSLVSHLVLARRIQTTVVKAKAA
                     RRLAERMVTLAKRNTLASRRAAVARLHNQDAVAALFEQLGPAFKDRQGGYTRIVRLGQ
                     RLGDGAETALLEWVNFVPQPKAKKKDKKEKPGEKPEAKAPEKKADKKTEKKAAEKSKA
                     KA"
     gene            5068..>5407
                     /locus_tag="PK634_13430"
     CDS             5068..>5407
                     /locus_tag="PK634_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98777.1"
                     /translation="MKRILWLGVFGIVALGPVPVGRGQADAPTATESVDSQAGAVQSL
                     EALLQTILSQEQEAARVQQQLIAAPDDVTRQELADRLKELRAEVEESRRQFERFAVDI
                     DLSPFVEEAKT"
BASE COUNT         1046 a         1719 c         1917 g          725 t
ORIGIN      
        1 cgccgcgcgt cgccggcgtc tgcgatgcct gcggcgggga gctctaccaa cgggcggacg
       61 acaccgagga aaccgtcctc aaccgcctcg aagtatttcg gaagcagacg gaaagcctga
      121 tcgagtacta cgagaagaag ggcgtcctcg tcaggatcaa cgccggtcgg aaccgcgagc
      181 tggtccaggc cgatatcctg gccgccctgc ggaagccggc ggccgggaca tgatcgtcat
      241 caagacccca gaggacctgg cccggatgcg ggtcagcggc cgcctggcgg ccgaggtgat
      301 cgaggagctg gcccggcgcg tagggcccgg ggtgacgacg aaggagttgg acgatctggc
      361 ccgggagctg atggcccagc gcggcggccg cagcgcgttc tttggatatc ggggttaccc
      421 cggacagacg tgcatctcga tcaacgacga ggtcgtgcac gggattcccg gtccccgccg
      481 gatccggctg ggggacatcg tcagcctgga cgtcggcgtg gtctacgagg gctacgtcgg
      541 cgataccgcg acaaccgtga tggtcggcgt cagcgatccc gaggtcgtgc agctcgtgag
      601 cacgggacgc cgggcgctgg aggcggccat cggcatggcg gtcgccggcg gccggctctc
      661 ggacatctcg cacgcgatcg agtcgacggc ccgggcggcc gggtacagcg tggtgcggga
      721 cttcgtgggc cacggcgtcg ggaaggcgat gcacgaggat ccccagatcc ccaacttcgg
      781 gccgccgggg cgcggcccga ggctgaagcc ggggatgacg ctggcgatcg agccgatgat
      841 caaccgcggc gggcacgagg tcgaggtgat ggacgacggg tggacggtcc ggacgcggga
      901 ccggagcctg tccgtgcatt tcgagcacac gatcgcggtc ggggaagggg cggccgaggt
      961 gctgacatgg ccgggcccga aataattcgg ctggacgcgc gggtggtttc tgtgataacc
     1021 aacgcggttt tccgggtcga actggccaac gggcatggcc tggtggccta cgcggcccgc
     1081 gccgaaaagg cccgggcgga agcggtgagt cccggcgacc gggtgcgcgt ggaattgtcg
     1141 ccctgcgaca tgtcgagggg gcggctggtt ttcagggaag aggaaaacgg atgaaggtac
     1201 gaagttcagt caagcgaatc tgcgagcggt gcaaagtggt gcgccggaaa cgggtggtgc
     1261 gcgtgatctg caataatccg cgtcacaagc agcgccaggg ttaacggagg aagacatgcc
     1321 cagagtgctc ggcatcgaga tacccggcaa gaagcgggtc gaatacgcgt tgcggtacat
     1381 ttacggcatc gggctgacgc gcgcgcgcca ggtcgtcgcc caggccggga tcaacccggc
     1441 caagaaggcg gacgagctga cggacgagga gatcaccggc atttccaacg ccctccaggg
     1501 ctacaacctg gaaggcgacc tccgccgcga ggtggcctcc aacatccgcc ggctcatcgg
     1561 catcggttgc taccggggtt cgcggcaccg caagggcctg cccgttcgcg gccagcggac
     1621 gagcaccaac gcgcggacgc gcaagggccc gcgcaagacg gtcggcgcgg tccgcggcaa
     1681 ggaagcccgg tccgaggtca agtcggataa agagtaattt caaaggacaa ggtgcgtatg
     1741 gcagacgaaa aagagaagca ggaagtcaag gcgccgcccg cggaagcggc cgtggcggaa
     1801 gccgccgctc cgggggccgt cccggccgag ggcgccgcgc ccgcggcgga gggcgaggcc
     1861 gccaaacccg tcaagcggaa ggccgtcaag ggcgcgcgca ccgtccccgt gggggtcgtg
     1921 tacgtccgcg cgacgttcaa caacacgatg gtcaccatca ccgacatgcg cggcgccgtg
     1981 gtctgctgga gcacggcggg ccgctgtggc ttcaagggtt cgcgccgcag cacggcctac
     2041 gccgccaccg tcgtcgccca ggaagccgcg cgacaggcga tcggcttcgg gatgcacgag
     2101 gtcgaggtgc ggatccaggg cccgggcgcc ggccgcgaat cggcgatccg cgccatccag
     2161 tccgccggcc tgacgatcac cgtcatcaag gacatcaccc cggtcccgca caacgggtgc
     2221 cggccgccca agcggcggcg cgtgtaggaa gttctccgag aaagcgaggc agagttcatg
     2281 ggcagatata cgggacccgt ttgcaggatg tgccgccggg agggcatgaa gctcttcctc
     2341 aagggcgacc ggtgtttcat ggccaagtgc ccgatcgaaa cggggcgctc cgcgcccggc
     2401 atgcacggcc agcgccgcag caagctttcg gactacggcg tgcaactgcg cgagaagcag
     2461 cgcctccgcc gcatgtacgg catgcaggaa ggccagttcc acctcttttt cacccgcgcg
     2521 ctccgcaagc gcggcgtcac cggcgagacc ctgctgcagt cgctcgagtg ccggctggac
     2581 aacgtcgtct tccggctggg cttcgccccg tcgcgccggg ccgcccggca gttcgtccgg
     2641 cacggccaca tccaggtcaa cggcaacaag gtggacatcc cgtccctcca gctgaaggcg
     2701 ggcgacatcg tgcaggtgcg cgaccgcgcg cagagccgcg agtacgccaa ccgccacatg
     2761 gaggtcgcgg agagccgcgg catcgtcggc tggctggcgc tcgaccgcgc caacgcccgc
     2821 ggcgaggtgc tgcacgcgcc cacccgcgac gagatcgcgc cggtggtccg cgagcagttg
     2881 atcgtcgagt tgtattcgaa gtaagcaggt ccgttaacac catatgggcc ggctaacgcc
     2941 cggccctggg aggaaagtca tgcccgtacg tctggcccgg ttcgaaatgc cgaagcgcat
     3001 cgtcaaggac gaggccgcgg cagcccccaa ctatggcaag ttcatcgcgg agcccttcga
     3061 ggccggctac ggccggaccc tcgggaactc gctccgccgc gtgctgctct cctcgatcga
     3121 aggcgcggcc atcgcgtccg tgaagatcga gggcgtccag cacgagttca gcaccctgcc
     3181 cggcatggtc gaggacgtca ccgacgtcat cctcaacctc aagcaggtgc tcctcaagat
     3241 gtacagccgc gcgccccgca agctgcggat cagcgtcaag ggccctggcg aggtcaaggc
     3301 cggcgacatc caggtggaca gcatggtcga ggtgctcaat cccgaccaca agatcgccac
     3361 gctggacaag gacggcaagt tcgacgcgga actcgagatc aagatcggcc gcggctactg
     3421 ccccgccgag tggaacaaga aggaggagca ggagatcggc ctgatcccga tcgactcgct
     3481 cttctccccg gtgcgccgcg tgaagtacac ggtggagaac acccgcgtcg gccgccgcac
     3541 ggactacgac cggctgatcc tcgaggtctg gacggacgga cgccagacgc cggacgagtc
     3601 gctcgccgtg tccgccgcgc tcctgcgcca tcacctcgac gtcttcgtca gcccggagca
     3661 cgaggagatc gagatcgccg aggcgacgcc cgagatcaac cccgagcgcg aggagctgcg
     3721 ccggcgcctg aacatcagcg tcaacgagat cgagttgagc gtgcgcgccg cgaactgcct
     3781 gaacaacgcc aacatcacga cggtcggcca gctggcccag aagaccgagg ccgagatgct
     3841 caagtaccgc aacttcggcc gcaagtcgct caacgagatc aagcagaagt tgagcgaact
     3901 cggcctgacg ctcggcatga gcttcgatgc cgacgtcctc aagccgctgg aagtcgaagt
     3961 caaggaagag ccggccgagt aaggccggac gggaagggac cctctcatgc gacatcgcaa
     4021 agacacgttg aaactgggcc gcacggcggc ccaccgggac gaactgctgg gcagcctggt
     4081 cagccacctc gtcctcgccc ggcgcatcca gaccaccgtc gtcaaggcca aggccgcgcg
     4141 gcggctggcg gagcggatgg tgaccctggc caagcgcaac accctggcct cgcgccgggc
     4201 ggcggtcgcc cgcctgcaca atcaggacgc cgtggccgcg ctgttcgagc agctcggccc
     4261 cgcgttcaag gaccggcagg gcggctacac ccgcatcgtg cgcctggggc agcggctggg
     4321 cgatggcgcc gagaccgcgc tgctggaatg ggtgaacttc gtgccccagc cgaaggccaa
     4381 gaagaaggac aagaaggaaa agcccggcga aaagcccgag gccaaggccc cggaaaagaa
     4441 ggccgacaag aaaaccgaga aaaaggccgc ggagaagtcg aaggccaagg cctgattccc
     4501 gtatccgttg aagaacggcg acgccccttg atatcatcca tcaaggggcg ttcgtttttt
     4561 gactggccaa cctgccttgc cgcgcggacg gccggcgctt gatgcgcaaa gcgggcgggg
     4621 gcgaaacctg cctgcaacgg cttcttcatc cgcagcaggg agacccccgt caacgcgacg
     4681 aggaccagca ataccccgtt gaggatcgtg tagagccagg agcgcgtcat caaggccccg
     4741 gccaggaaca ggacgcagtt ggcggcgtaa agcagcggca gaccggattt cggtgtacac
     4801 cagcaggaac aagccgttcg agcgtggggc gagggcgggg ttcgcgtggc cggagccacg
     4861 gggaaagcgc cggcatgtcc gatcgatgca aggaggagcc gccgcgccat gccggggaat
     4921 aacagggcct tcatggcgga aaactgccga aaggcgcggc gcaagacaag catggagaaa
     4981 gggagccgtg tcggcagtga tggatttact tgcagggagc ggtgtgctta attgcggaca
     5041 tacaaggctt ggtgaagggg ggggcgcatg aaacgtattc tttggctggg agtgtttggg
     5101 atcgttgcgc tgggtccggt tcctgtcggc cgcggacagg ccgatgcgcc gacggcaacg
     5161 gagtccgtgg actcccaggc aggcgccgtc cagtccctgg aagccctgct gcagaccatc
     5221 ctgagccagg aacaggaggc ggcccgggtc cagcagcaat tgatcgcggc gccggacgac
     5281 gtgacccggc aggaactcgc ggaccgcctg aaggaactgc gcgcggaagt cgaggagtca
     5341 cgccgccagt tcgagcggtt cgccgtggac atcgacctgt cgcctttcgt ggaggaggcc
     5401 aagaccg
//
LOCUS       DAOOTK010000172         5353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9572_length_5353_cov_5.735561, whole genome shotgun sequence.
ACCESSION   DAOOTK010000172 DAOOTK010000000
VERSION     DAOOTK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9572_length_5353_cov_5.735561"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PK634_13435"
     CDS             complement(<1..892)
                     /locus_tag="PK634_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98778.1"
                     /translation="MAWFRFFLPALAADHFVENFDTFTNASYLTGALVLSSGTWTGLS
                     VLGETASASRGGTGRAVRINDDTAGAQLATPALDTVGTVSFYWRELNAGGGTFRVQKS
                     VAGGAYTDVGTLVYTGTTFTLYSTNVNDSSSAIRIRILNDNQYGHLIIDDMTVTDYSA
                     GSTNTPPALNAIGAKSVGVGSNLQFNVTATPTDGDTVTLTASNLPAGAAFFPTNEAAS
                     FLWTNAAPTGTYSVTFNAADNDGSDEETITITVSPAGAQSPPALNAIGAKSVGVGSNL
                     QFNVTATPTDGDTVTLTASNL"
     gene            1063..1503
                     /locus_tag="PK634_13440"
     CDS             1063..1503
                     /locus_tag="PK634_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOW98779.1"
                     /translation="MARTVMLLGALLLAASCATTPREARRPPDRAMTMTVTAYCPCGK
                     CCGWRRNWLFRPVIRSGPRRGKPKAVGVTASGTKARPGTIAADTRLLPFGTVLHVPGY
                     GYGRVEDTGGDIQGHRLDVFFKRHSTAEAWGVQRLPVRVWYAPR"
     gene            1587..1883
                     /locus_tag="PK634_13445"
     CDS             1587..1883
                     /locus_tag="PK634_13445"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOW98780.1"
                     /translation="MSRWVWMAVLAAGLLAVPVMAQEATQAAALQDVESTLVEAVGYD
                     AATQTLTVKLVTDGSVYEYFGVPQEVYDQFMAAESKGNFFAKNIKNVYKFQKKQ"
     gene            complement(2001..2264)
                     /locus_tag="PK634_13450"
     CDS             complement(2001..2264)
                     /locus_tag="PK634_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOW98781.1"
                     /translation="MNIYVGNLSFKATDADLRAAFEPFGQVQSAKIVEDRETGRSRGF
                     GFVEMPNDEEGRKAIEGVNNKDIAGRAVRVNEARPREDRPPRG"
     gene            complement(2407..3390)
                     /locus_tag="PK634_13455"
     CDS             complement(2407..3390)
                     /locus_tag="PK634_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOW98782.1"
                     /translation="MRSVRNVAGFLRQRWPVWAPRLRHALLWAALAAAAAVFAAWRIV
                     SEWDRHPLTILVLGSDTRSETEPGRSDAIFVLRADPKRRSMSGLSLPRDLYVPIRGLP
                     TLLTNRINSALFFGDYFGVPGGGRRAARETVAHLLGVPIDGVVVLGFPIVQDVVDSIG
                     GVEVYIENLVVDRSFFPMQGGHGYAVWFEPGWNYLDGRRALEFVRLRKPDTDFGRMDR
                     NRQFFTALQGALEGRAGFWQRLRLFARIKSRIRSDMGFGRKLRAAWVFSRCADGPIRW
                     NTLSRNEVEPGTTAQGAQILVPGPGVLERAGQQLLEERPPRLARAPGEPGR"
     gene            3542..4408
                     /locus_tag="PK634_13460"
     CDS             3542..4408
                     /locus_tag="PK634_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98783.1"
                     /translation="MSDIQLQCLGCNKAFLVPEDQAGLLVACPQCNRKIAVPVPSDQI
                     ERAPKLAIRREEPAAASGMNCPSCGATMPPGAVLCVECGYDVRKGARLGETLGWPPYV
                     RWLIAGAGVIIVVMLVKTFLRPHRISEIAAPPPAVQAPPKPAAAPAETNAPAAAASEP
                     APAPLPVPAAPAETNAPAAAPQVSPEELAQLEADYRASLKGRLDQQQPLYRPGDLVEL
                     RKVSGLVIRGKLKAVEARGAVVVREEGETVVALADLDRESRVRCDAEFREKFIEFIVK
                     KNMRSIQEPAAP"
     gene            4509..>5353
                     /locus_tag="PK634_13465"
     CDS             4509..>5353
                     /locus_tag="PK634_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOW98784.1"
                     /translation="MKEKRSLQELEAEVAELTRLLVQSERAQSLGALSGGVAHHFNNL
                     LSVILGYSSFVLNRESLSKEAADALRKIAEAAQRGRRLTEEILAFLGGESEEVKPCSV
                     HDTLRHVLSLLESQTGSAIRVRTHFDARRDTVLSVPSAIHQIVFNLLTHAIDGMPGGG
                     WLEVSTANERVGGEAGEREYLQLDVVDLSEKGKEPRRAAPETGARKSLKLSSAYGIVG
                     RLEGSMVITEQGDRGSRVRVLLPVGSAGEQAPAEARSRRRLTPRVIWVVDDDPIFREM
                     CRQLLS"
BASE COUNT          839 a         1797 c         1896 g          821 t
ORIGIN      
        1 ggaggttgct ggcggtgagg gtcaccgtgt cgccgtcggt cggcgtggcg gtgacgttga
       61 actgcaggtt gctgccgacg cccacggact ttgcgccgat cgcgttcagc gccggcgggg
      121 attgcgcccc ggccgggctg acggtgatcg tgatggtttc ctcgtcggag ccgtcgttgt
      181 cggcggcgtt gaaggtgacg gaataggtgc cggtgggcgc ggcattggtc cagaggaagg
      241 aggcggcctc gttggtcggg aagaaggcgg cgccggcggg gaggttgctg gcggtgaggg
      301 tcaccgtgtc gccgtcggtc ggcgtggcgg tgacattgaa ctgcaggttg ctgccgacgc
      361 ccacggactt cgccccgatc gcgttcagcg ccggcggcgt attggtcgag ccggcgctgt
      421 agtccgtcac ggtcatgtcg tcgatgatga ggtgcccgta ctggttgtcg ttcaggatgc
      481 ggatgcggat ggcgctgctg gagtcgttca cgttggtgct gtagagggtg aacgtcgtgc
      541 cggtgtagac cagcgtgccc acgtccgtgt acgcgccgcc cgcgacggat ttttgcaccc
      601 ggaacgtccc cccgccggcg ttgagttcgc gccagtaaaa cgaaaccgtg ccgaccgtat
      661 ccaacgcggg cgtggccaac tgggcgccgg ccgtgtcgtc gttgatccgg actgcccggc
      721 ccgtgccgcc gcgcgacgcc gaggcggttt cgcccaaaac ggagaggccg gtccatgttc
      781 cgctggaaag caccagggcg ccggtcaggt agctggcatt ggtaaacgtg tcgaaattct
      841 cgacgaaatg atcggccgcc agggctggca agaaaaagcg gaaccaagcc aagataagcg
      901 ctcctgacgc gcggcggagc agtttcacaa catgaaacct ttcagttttc tggcgtaaag
      961 ttaaagggtg accataccct ccaagcccgg tggagttaag ggcttggacc ggggcgacgg
     1021 ttgcggttga tttttccccc gcgcgagggc atggtgcggc ggatggcccg aaccgtcatg
     1081 ctgctgggcg cgctgctgct ggcggcgtcc tgcgccacga cgccgaggga ggcccggcgg
     1141 ccgccggacc gggcgatgac catgaccgtc acggcgtact gcccctgcgg gaaatgctgc
     1201 ggctggcggc gcaactggct cttccgcccg gtcatccgtt cgggcccgcg gcgcggcaag
     1261 cccaaggcgg tcggcgtgac cgcgtccggg acgaaggcgc ggcccggcac catcgcggcg
     1321 gatacccgcc tcctgccgtt cggcactgtc ctgcatgtgc caggatacgg ttacgggcgc
     1381 gtcgaggaca ccggcggcga catccagggg catcggctgg acgtcttctt caagcgccat
     1441 tccacggccg aggcctgggg cgtccaacgg ctgccggtcc gtgtgtggta cgctccccgc
     1501 tgaccccgga acacccctcc gtttccacgc gacaagcggg ccgggatggt gtatagttcg
     1561 cccggtttca aaataggagg aaacctatga gtcgctgggt atggatggcg gtgttggcgg
     1621 cgggattgct ggcggttccg gttatggcgc aggaagcgac acaggccgcg gcgttgcagg
     1681 atgtcgagtc caccctggtg gaggcggtgg gctacgatgc ggccacgcag accctgacgg
     1741 tcaagctggt cacggacggc agcgtgtacg agtacttcgg cgtgccgcag gaagtctacg
     1801 accagttcat ggcggcggag tccaagggga acttcttcgc caagaacatc aagaacgtct
     1861 acaagttcca gaagaagcaa tagcgcgttt ccgcctgcgg gcggtgcgcg gacgcgcgcg
     1921 ccgcgggccg gatgacagtg ttgcaaagcc cgccccgccg gaccggcgga gcgggctttt
     1981 ttcgtgatgg gccggcgggt ttatccgcgc gggggacggt cttcgcgcgg acgcgcctcg
     2041 ttgacgcgga cggcgcggcc ggcgatgtcc ttgttgttca cgccctcgat ggccttgcgc
     2101 ccctcttcgt cgttgggcat ttccacgaac ccgaagcccc gggagcgccc cgtttcccgg
     2161 tcttctacga tcttggccga ttggacctgg ccgaagggtt cgaacgcggc ccggaggtcc
     2221 gcgtcagtgg ctttgaagga caggttcccg acgtatatgt tcactctcgt tgctcctgcg
     2281 tgctcgctgc cggttcccgc ctcccgtcaa ggggccggca gcaggcccct cgccgagtgt
     2341 gccgaaagat agggaagtcc cggcggcccg gcaagcccat aatcggggcg gcatccgggc
     2401 gcgcgatcag cgccccggct cgcccggggc ccgcgccagg cggggcggcc gttcttcgag
     2461 gagctgctgg ccggcccgct ccaggacgcc gggcccgggc acgaggatct gcgcgccctg
     2521 cgcggtggtc cccggttcga cctcgttgcg ggagagcgtg ttccagcgga tcggcccgtc
     2581 cgcgcagcgc gagaagaccc atgccgcgcg cagcttccgg ccgaagccca tgtcgctccg
     2641 gatccggctc ttgatccggg cgaacagccg gagccgctgc cagaatcccg cgcggccttc
     2701 cagggcgccc tgcagcgccg tgaagaactg gcggttccgg tccatccgtc cgaaatcggt
     2761 gtccggcttg cggagccgca cgaactcgag ggcgcgccgc ccgtcgagat agttccagcc
     2821 cggctcgaac cataccgcgt agccgtgccc gccctgcatg ggaaagaacg agcggtcgac
     2881 gaccaggttt tcgatgtaca cctccacgcc gccgatcgag tccacgacat cctgcacgat
     2941 cgggaagccc agcacgacca ccccgtcgat cgggacgccg agcaggtgcg ccaccgtttc
     3001 gcgggccgcc cgccgcccgc cgcccggcac gccgaagtag tccccgaaga aaagggcgga
     3061 gttgatccgg ttggtcagca gggtgggcag gccgcggatc ggcacgtaca ggtcgcgcgg
     3121 gagggagagc ccgctcatgc tccgcctttt cgggtcggcc cgcaggacga aaatggcgtc
     3181 ggaccgcccc ggctcggttt ccgagcgggt atccgagccc aggaccagga tcgtaagcgg
     3241 atgccggtcc cattccgaca cgatgcgcca ggccgcgaac acggcggcgg cggcggccag
     3301 ggcggcccag agcagggcgt gtcgcagccg cggggcccac accggccacc gctgccggag
     3361 gaaaccggcg acatttcgaa cggatctcat tcaggaaaac ccgcccacga tagacccccg
     3421 gccccttttt gtaaaggggc gcccggcggc ttgactttgg cggcgtgccg cgtccagaat
     3481 gaaaaaaatt atttcaaccg gggcctgtcc cgcggcggcc ccggggacaa aggcggagct
     3541 tatgtcggac atacaacttc aatgcctcgg ctgcaacaag gccttcctcg tgccggagga
     3601 ccaggccgga ctcctcgtcg cctgccccca gtgcaaccgc aagatcgccg tcccggtgcc
     3661 gtcggaccag atcgagcggg cgccgaagct cgcgatccgc cgggaggagc ccgccgcggc
     3721 cagcggcatg aactgcccgt cctgcggcgc gaccatgccg cccggcgcgg tgctctgcgt
     3781 cgaatgcggt tacgacgtcc gtaaaggcgc ccggctcggc gagaccctgg gctggccgcc
     3841 ctacgtgcgc tggctgatcg ccggcgcggg cgtgatcatc gtggtcatgc tggtcaagac
     3901 cttcctgcgc ccccacagga tttcggagat cgccgcgccg ccgccggccg tccaggcgcc
     3961 gccgaagccc gccgccgccc cggcggagac gaatgcgccg gccgccgcgg cttccgagcc
     4021 ggcgcccgcg cccctgccgg ttcccgcggc ccccgccgag acgaatgcgc cggccgccgc
     4081 gccccaggta tcccccgagg aactggccca gctggaggcc gactaccgcg cttcgctcaa
     4141 gggccggctc gaccagcagc agcccctcta ccggccgggc gacctggtgg aactgcgcaa
     4201 ggtcagcggg ctggtgatcc gcggcaagct gaaggcggtg gaggcccggg gcgcggtcgt
     4261 ggtgcgcgag gagggcgaga ccgttgtcgc gctggccgat ctcgaccggg aaagccgcgt
     4321 ccgctgcgac gcggaattcc gcgaaaagtt catcgagttc atcgtcaaga agaacatgcg
     4381 ctccatccag gagccggcgg cgccgtaggc cggcggagcg agcgcgcgcc cggatatcaa
     4441 caaaaacggg acccgcgggt attctcctcc aacggggccg ggcgcggacg tctcggcaag
     4501 atgggaccat gaaagaaaaa cgaagccttc aggaactcga ggccgaggtg gcggaactga
     4561 cgcggctgct cgtccagtcc gagcgggccc agtcgctggg cgcgctgtcg ggcggcgtcg
     4621 cccaccattt caacaacctg ctctcggtca tcctcggcta ctcctccttc gtgctcaacc
     4681 gggaatccct gtcgaaggag gccgccgacg cgctgcgcaa gatcgccgag gccgcccagc
     4741 gcgggcggcg gctgaccgag gagatcctcg cgttcctggg cggcgagagc gaggaggtca
     4801 agccgtgcag cgtgcacgac acgctgcgcc acgtgctctc gctgctcgag tcgcagaccg
     4861 gcagcgccat ccgcgtgcgc acccatttcg acgcccggcg cgacaccgtc ctgtccgtcc
     4921 ccagcgccat ccaccagatc gtgttcaacc tcctgaccca cgcgatcgac ggcatgcccg
     4981 gcggcgggtg gctggaagtc agcaccgcga acgaacgggt cggcggcgag gccggcgagc
     5041 gcgagtatct gcagctcgac gtcgtggacc tgtcggagaa ggggaaagag ccgcgccgcg
     5101 ccgcgcccga gaccggcgcg cgcaagagcc tgaagctctc cagcgcctac ggcatcgtcg
     5161 gccggctcga aggctccatg gtcatcaccg agcagggcga ccgcgggagc cgcgtgcgcg
     5221 tgctgctgcc cgtaggctcg gccggcgagc aggcccccgc cgaggctcgg agccgccggc
     5281 gcctgacccc gcgcgtgatc tgggtggtgg atgacgaccc gatcttccgg gaaatgtgcc
     5341 gccagttact ctc
//
LOCUS       DAOOTK010000173         5264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9795_length_5264_cov_4.199270, whole genome shotgun sequence.
ACCESSION   DAOOTK010000173 DAOOTK010000000
VERSION     DAOOTK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_5264_cov_4.199270"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK634_13470"
     CDS             complement(<1..247)
                     /locus_tag="PK634_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98785.1"
                     /translation="MGQKVNPIGFRVSVNKDWRSRWYAEKRQFGDLLHEDLLIRELVK
                     KYSDFIAYPIRFGEKGEAPKEDAEALNTMKALWKRAKS"
     gene            complement(277..624)
                     /gene="rplV"
                     /locus_tag="PK634_13475"
     CDS             complement(277..624)
                     /gene="rplV"
                     /locus_tag="PK634_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOW98786.1"
                     /translation="MDIAAKTKFARISASKAMDLARAVRGQPVGRAIQVLARSQRKGA
                     VLMLKTLKSAIANAENNAKLEADKLYVRDAAVEQGPAMKRFWPRSRGMVSPVLRRMSH
                     IRIVLTDEKPSAK"
     gene            complement(631..1149)
                     /locus_tag="PK634_13480"
     CDS             complement(631..1149)
                     /locus_tag="PK634_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98787.1"
                     /translation="MSRKKAAPERRPRPAVTNAAESVESREARLAALALAGQNEALRG
                     ERDDLRKQVRQLAESLAAVRIEADQYRARLDQGGLEPAEPAAPDLAGEWKVMKASPDL
                     RMVVVDGGAQQGLRNGMTMFVLRGDRPVARLIVVDARAAVAGAVVEESRDGMEPETGD
                     RVIPARPGMSLR"
     gene            complement(1282..1566)
                     /gene="rpsS"
                     /locus_tag="PK634_13485"
     CDS             complement(1282..1566)
                     /gene="rpsS"
                     /locus_tag="PK634_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOW98788.1"
                     /translation="MSRSLKKGPFVEARLLKKVELLNRSGAKTPIKTWSRRSMIMPEF
                     LGHTFAVHNGKTFVNVFVTENMVGHRLGEFSPTRIFRKHGAATDKTVALK"
     gene            complement(1570..2394)
                     /gene="rplB"
                     /locus_tag="PK634_13490"
     CDS             complement(1570..2394)
                     /gene="rplB"
                     /locus_tag="PK634_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOW98789.1"
                     /translation="MALISHKPVTPSERFTSLLSRDDVTQKRPEKGLRSFRKSRAGRN
                     SMGRVTIRHRGGGHKRFLRHVDFLRDKAGIPAKVVAIEYDPNRSARIALLHYKDGEKR
                     YILAPLGLAVGRTVVSGPGAEPLEGNALPLVEIPLGVPVHCIEMVPGRGAQLVRGAGM
                     AAQIMAREGDYAHIRLPSGEIRKIPIRCMATVGQVGNLDHESVSLGKAGRKRWKGIRP
                     TVRGVVMNPVDHPMGGGEGRTSGGGHPVSPWGKLTKGKKTRNPRRVSSRMILKRRK"
     gene            complement(2408..2692)
                     /gene="rplW"
                     /locus_tag="PK634_13495"
     CDS             complement(2408..2692)
                     /gene="rplW"
                     /locus_tag="PK634_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOW98790.1"
                     /translation="MKDPRTIIRKVLLTEKGTAMKEKENQYLFEVSPDANKIEIRLAV
                     EKLFKVKVTKVNTLNRSGKQKRLRTMKYGWTADSKRAIVTLKEGDKIEFS"
     gene            complement(2689..3333)
                     /gene="rplD"
                     /locus_tag="PK634_13500"
     CDS             complement(2689..3333)
                     /gene="rplD"
                     /locus_tag="PK634_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOW98791.1"
                     /translation="MSKLPLLNMKGERVGEVGLADDALPKGKGGQAVHDAVTAHLAAL
                     RAGTASTKKRGAVAGGGAKPYKQKGTGRARAGSIRSPIWRGGGIVFGPHPRDYGIRLP
                     RKVLQLAFCRAVAEKAAAGQVAVLDRIAVPEPKTKHVQAMLEALKAERGALLVLDTAD
                     ANVTRAARNITGVGVTTAAALHTYEVMAAPMLVVSRADLEQIEQRMQKRLGDAP"
     gene            complement(3330..3983)
                     /gene="rplC"
                     /locus_tag="PK634_13505"
     CDS             complement(3330..3983)
                     /gene="rplC"
                     /locus_tag="PK634_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOW98792.1"
                     /translation="MMNGLLARKIGMTQVFDDQGVHVPVTVLQVGPCVVVQRKTKAAD
                     GYEAVQVGLGSKKESRLTKAEQGHFKKAGQAPSAVLREFRLDEGEDLKPGESLTVSIF
                     DGVSYVDVEGVTKGKGFQGVVRRHSMVGGGAAHGSMMHRRSGAIGNRTWPARIFKNKR
                     MPGHMGHVQVTMQNLKVVQVRPEDHALLVRGAVPGPTGALLVVRKAVKKAAKAAKKS"
     gene            complement(4007..4315)
                     /gene="rpsJ"
                     /locus_tag="PK634_13510"
     CDS             complement(4007..4315)
                     /gene="rpsJ"
                     /locus_tag="PK634_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOW98793.1"
                     /translation="MSGQRIRIRLKGFDHRVLDQSAGDIVETAKRTGARVAGPIPLPT
                     RIERYSVNRSPHADKKSMEQFEIRTHKRLLDIIDPTARTVDELKKLNLPAGVDITIKI
                     "
     gene            complement(4323..>5264)
                     /gene="fusA"
                     /locus_tag="PK634_13515"
     CDS             complement(4323..>5264)
                     /gene="fusA"
                     /locus_tag="PK634_13515"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOW98794.1"
                     /translation="VAALKQITTGDTLCAEHQPIALERIEFPEPVMAMAIEPRTAADR
                     AKLAETLASLSEEDPTFKVTADAETGQTLIKGMGELHLEIIRDRMLREFKVQANAGKP
                     MVAYRETIRAPARAEQLFQREIGGHGQHGHVVIEIEPRARAAGNAIEFDVSANDIPAP
                     FREAVEQGLQDGLLTGVVGNYPLTDVQVRVVGGSFHPVDSSEVGYRTASVLALREAAQ
                     KAQPTLLEPIMKMEIITPEEFLGDVLGDVNSRRGQVREMEAREGVQIVRAEVPLAELF
                     GYTTSLRSLSKGRASCSMEPYHFEIVPDSLQKDILNR"
BASE COUNT          717 a         1909 c         1635 g         1003 t
ORIGIN      
        1 cgctctttgc gcgcttccac agcgccttca tcgtgttcag cgcttctgcg tcctctttgg
       61 gcgcctctcc tttctcgccg aagcggatcg gataggcgat aaaatccgag tacttcttga
      121 ccagctcgcg gatcagcaga tcctcgtgca gcaggtcgcc gaactgccgc ttctcggcat
      181 accaccgaga acgccagtcc ttgttgacgc tcaccctgaa cccgatggga ttgactttct
      241 gacccactcc cggtctcctt tccagcaaaa gccgctttat tttgccgacg gtttttcgtc
      301 cgtcaataca atcctgatgt gactcatcct ccgtaacacg gggctgacca tgccgcgcga
      361 gcggggccag aagcgcttca tcgcgggccc ctgctcgacc gccgcgtcgc ggacgtacag
      421 cttgtccgct tcgagcttgg cgttgttctc ggcgttcgcg atggccgatt tcagcgtttt
      481 tagcatgagg accgcgccct tgcgctggct gcgcgccagc acctggatcg cccggcccac
      541 gggctgaccg cggaccgcgc gcgccaagtc catcgccttg ctcgcgctga tcctcgcaaa
      601 tttcgtctta gctgctatgt ccatggcatc tcacctcaag ctcatgcccg gccgcgccgg
      661 gatcacccgg tcgccggtct cgggctccat cccgtcccgc gactcttcca ccacggcccc
      721 ggccaccgca gcccgggcgt ccacgacgat cagccgggcc accggccggt cgccgcgcaa
      781 cacgaacatc gtcatcccgt tccgcagccc ctgctgcgcc cccccgtcca cgaccaccat
      841 ccggaggtcc gggctggcct tcatcacctt ccactcgccc gccaggtcgg gggccgccgg
      901 ttccgccggc tccaggccgc cctggtccaa ccgcgcccgg tactggtccg cctcgatccg
      961 gacggccgcc agcgactcgg ccagctgccg gacctgcttc cgcaggtcgt cccgctcacc
     1021 ccgcagggcc tcgttctgcc cggccaaggc gagggcggcc agccgcgctt cccggctttc
     1081 caccgattcc gcggcgttcg tcaccgcggg gcggggcctt ctttccggcg ccgccttttt
     1141 cctgctcaaa gaacaacgtt cactgcgtcc cagccaccac ccgccggccc cggccagcgc
     1201 cgcgaccagc aggccgcccg cgatccacca ccccgcgccc cgccccggcc ctgtggcccg
     1261 cccctccagg gtgcgcctcg cctacttcag cgccacggtc ttgtcggtgg ccgcgccgtg
     1321 cttgcggaag atccgcgtgg gcgaaaactc gcccaggcgg tgtccgacca tgttttccgt
     1381 cacgaaaacg ttcacgaacg tcttgccgtt gtgcacggca aacgtatgcc cgaggaattc
     1441 cggcatgatc atcgagcggc gcgaccaggt cttgatcggc gtcttcgcgc cggaccggtt
     1501 cagcagttcg accttcttca gcagccgggc ctcgacgaag ggcccctttt tcagcgatcg
     1561 cgacatggct tatttcctcc gcttgaggat catccggctc gacacgcgcc gggggttccg
     1621 ggtcttcttg cccttggtca gcttgcccca cggcgacacg ggatgcccgc cgccggaggt
     1681 ccggccttcg ccgccgccca tcgggtggtc caccgggttc atcaccacgc cgcgcaccgt
     1741 cgggcggatg cccttccagc gcttgcggcc ggccttgccc agcgaaacac tctcgtggtc
     1801 caggttgccg acctggccga cggtcgccat gcagcggatc gggatcttgc ggatctcgcc
     1861 ggagggcagc cggatgtggg catagtctcc ttcgcgggcc atgatttgcg ccgccatgcc
     1921 cgcgccgcgc accagctgcg cgccgcggcc ggggaccatc tcgatgcagt gcaccggcac
     1981 cccgagcggg atctcgacca gcgggagggc gttgccctcg agcggctcgg cgcccggccc
     2041 ggaaaccacc gtgcggccga ccgccaggcc gagcggcgcg aggatgtagc gcttttcgcc
     2101 gtccttgtag tgcagcaggg caatgcgcgc cgaccggttg ggatcgtact cgatcgccac
     2161 gaccttggcc gggatcccgg ccttgtcgcg caggaaatcc acgtgccgca ggaaccgctt
     2221 gtgcccgccg ccgcgatgcc ggatcgtcac ccggcccatg ctgttgcggc cggcgcgcga
     2281 cttgcggaag gaccgcaggc ccttctcggg ccgcttctgg gtcacgtcgt cccggctcaa
     2341 cagcgaggtg aaccgctcgc tgggggtgac gggtttatgg gatatcagag ccatgacatc
     2401 aatcctttca actgaattcg atcttgtcgc cttccttgag cgtcacgatg gcgcgcttgg
     2461 aatcggccgt ccacccgtac ttcatggtcc gcagccgctt ctgcttgccg gaccggttca
     2521 gggtgttgac cttcgtgacc ttgaccttga agagtttttc gaccgccagt cggatttcga
     2581 tcttgttcgc gtccgggctc acttcgaaaa ggtactggtt ctccttttcc ttcatggcgg
     2641 tgcccttctc cgtcagcagc accttccgaa tgatcgtgcg cgggtccttc atggcgcgtc
     2701 tccaagccgt ttctgcatcc gttgttcaat ctgctcgagg tcggcgcggg agaccaccag
     2761 catcggggcc gccatgacct cgtaggtgtg cagcgcggca gccgtggtca cgcccacgcc
     2821 cgtgatgttc cgggcggcgc gcgtcacgtt cgcgtccgcg gtgtcgagca ccagcagcgc
     2881 gccccgctcc gccttcaacg cttcgagcat cgcctgcacg tgcttcgtct tcggctcggg
     2941 caccgcgatg cggtccagca cggccacctg gccggcggcg gccttttcgg ccacggcccg
     3001 gcagaacgcc agttgcagca ccttgcgggg cagcctgatg ccataatccc gcggatgcgg
     3061 gccgaacacg atgccgccgc cgcgccagat gggcgaccgg atggagcccg cgcgggcgcg
     3121 gccggtgccc ttctgcttgt acggcttggc gccgccgccc gccacggccc cgcgcttctt
     3181 tgtggacgcg gtgccggcgc gcagcgcggc caggtgcgcc gtcacggcgt cgtggaccgc
     3241 ctgtccgccc ttgcccttcg ggagcgcgtc gtcagccagg ccgacctcgc ccacccgttc
     3301 gcccttcata ttaaggagag gtagtttgct catgatttct tcgccgcctt cgccgccttc
     3361 ttgacggcct tgcgcaccac cagcagggcg cccgtcggac cgggcaccgc gccgcgcacg
     3421 agcagcgcgt ggtcctccgg acggacctgc acgaccttca ggttctgcat ggtcacctgg
     3481 acgtggccca tgtgcccggg catgcgcttg ttcttgaaaa tacgggcggg ccaggtgcgg
     3541 ttgccgatgg cgccgctgcg ccggtgcatc atggacccgt gcgccgcgcc gccgccgacc
     3601 atgctgtggc gccggacgac gccctggaag cccttgccct tcgtcacgcc ctcgacgtcc
     3661 acgtaggaga cgccgtcgaa gatggaaacc gtcagcgact cgccgggctt gagatcctcg
     3721 ccttcgtcca gccggaactc gcgcaacacg gcgctcggcg cctggccggc cttcttgaag
     3781 tggccctgtt ccgccttcgt caggcgcgat tccttcttgc tgcccagacc gacctgcacg
     3841 gcctcgtagc cgtccgcggc cttggtcttg cgctgcacca cgacgcaggg ccccacctgg
     3901 agcaccgtca cgggcacgtg cacgccctgg tcgtcgaaga cctgggtcat tccgattttt
     3961 ctcgcgagta atccgttcat aataggcctt ataggtcgta gagggcttat atcttgatgg
     4021 taatgtccac gccggccggg aggttgagct tcttgagctc gtccaccgtg cgggccgtcg
     4081 ggtcgatgat gtcgagcagc cgcttgtgcg tccggatctc gaactgctcc atggatttct
     4141 tatccgcgtg ggggctgcgg tttacgctgt agcgctcgat gcgcgtcggc aggggaatcg
     4201 ggccggccac ccgcgcgccc gtgcgcttgg cggtctcgac gatgtccccc gcggactggt
     4261 ccagcaccct gtggtcgaat cccttgagtc gtatacgtat gcgttgtccg ctcatgcttg
     4321 tctcaccggt ttaagatatc cttctgcagc gaatccggaa cgatctcgaa atgatatggc
     4381 tccatcgagc agctggcgcg ccccttggac aacgagcgca gtgacgtcgt gtacccgaac
     4441 aactccgcca gcggcacctc ggcgcggacg atctgcacgc cctcccgcgc ctccatctcc
     4501 cgcacctgcc cgcgccggct gttgacgtcg ccgagcacgt cgccgaggaa ctcctcgggg
     4561 gtgatgattt ccatcttcat gatgggctcc agcagggtcg gctgggcctt ctgggcggcc
     4621 tcgcgcagcg cgaggaccga cgccgtccgg taaccgactt cgctggaatc cacgggatgg
     4681 aaactgccgc ccaccacccg gacctggacg tccgtcagcg ggtaattgcc cacgacgccg
     4741 gtcagcaggc cgtcctgcag cccctgctcc accgcttcgc ggaacggggc cgggatgtcg
     4801 ttggcggaaa catcaaattc aatcgcgttg ccggccgcgc gggccctcgg ctcgatctcg
     4861 atgaccacgt gcccgtgctg gccgtgcccg ccgatctcgc gctggaagag ctgctccgcg
     4921 cgggcgggcg cgcggatggt ctcgcggtag gcgaccatcg gcttgccggc gttggcctgg
     4981 accttgaact cgcggagcat ccggtcgcgg atgatctcga ggtgcagttc gcccatgccc
     5041 ttgatgaggg tctggccggt ctccgcgtcc gccgtaacct tgaaggtcgg atcctcctcg
     5101 gacagcgagg ccagggtctc cgccagcttc gcgcggtccg ccgccgtgcg gggctcgatg
     5161 gccatggcca tgacgggctc cgggaactcg atgcgctcga gggcgatcgg ctggtgctcg
     5221 gcgcacaggg tatcgcccgt cgtgatctgc ttgagcgcgg cgac
//
LOCUS       DAOOTK010000174         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9813_length_5256_cov_5.845991, whole genome shotgun sequence.
ACCESSION   DAOOTK010000174 DAOOTK010000000
VERSION     DAOOTK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9813_length_5256_cov_5.845991"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(394..1035)
                     /locus_tag="PK634_13520"
     CDS             complement(394..1035)
                     /locus_tag="PK634_13520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOW98795.1"
                     /translation="MKRSASEWAVFFNGHAPQYDGNCFTQNTQSEIAFLIRKLGLGPG
                     MSLLDVGCGTGRHAIELARRGFRVTGVDISSGMLSQAREKAAAAGVAVTWIETDATRF
                     ATDSSFDAAICLCEGAFGLLGARDDALGQPAAILRNVSASMNPCARCLFTVLNGYRMA
                     RRHGQDDVASGGFDPLTLTERSDCEAGGRTSLRERGFVPTELRLLFRAAYPAA"
     gene            complement(1032..1577)
                     /locus_tag="PK634_13525"
     CDS             complement(1032..1577)
                     /locus_tag="PK634_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOW98796.1"
                     /translation="MVLRALRAGDARSVHEAYASDPEATRFLAWRAHKSLAETRRYLR
                     QTRITAQQGTEHLWALLLKRGGLIGAAGIRPCGSRIEIGYVLGRAWWGRGYATEAARA
                     LTAWAMRQPSVYRVWATCDVDNKASARVLEKIGMTREGVLRKWIVRPALGPAPRDSFC
                     YSIVKAREKRRAGNRQGAARS"
     gene            complement(2347..3042)
                     /locus_tag="PK634_13530"
     CDS             complement(2347..3042)
                     /locus_tag="PK634_13530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HOW98797.1"
                     /translation="MSRDPRLQDMLAHVVPFAAWLALIYLPGSPSGWTYAVRAAAGLI
                     LFLGLRPWRGYAPLRARNLVPAAWVGVLVFAVWVLPETGWAARWPALQEFYLKYAVLP
                     WGRLPEAVSPPPYAPETCGWPLAIVRLLGSAFVISFIEEFFWRGFIYRWITARDFRSV
                     DLGVFVRNAFFITALLFGLEHDRWLVGVLAGLAYGALAVKTRDLWAAGMAHAITNFLL
                     GLYVLAAGHYGFW"
     gene            complement(3039..4601)
                     /locus_tag="PK634_13535"
     CDS             complement(3039..4601)
                     /locus_tag="PK634_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOW98798.1"
                     /translation="MAASTTTDGAGSLVEAFRAVARASPEAVAVSFGGRVTTYAALDA
                     ASDRAAAALAARGVRPGDRIGLYCINSDAFAVAYFGIVKAGAVVVPVNLLLNPKEVAF
                     ILDDSGARGLIYFEAFAEGVRSLKPAVRSLEFAVCVGAQNPLGDMPFASILSAGGACP
                     CPPVNPAEDLVAIIYTSGTTGRPKGAMLTHRNLVSNVRSVLSALRFEPGRDVVLVVLP
                     MFHAFAATAAMLSPLLAGCMIVPLPKFDPAQAADAIAASGATIFMGVPSMFNVLLRLP
                     AEYTGKLASLRFAISGGAALPVEIMRQFEERFGKAIYEGDGPTECSPVTCVNPIGGRR
                     KPGTVGLPVPGVEMKIMDEQGRELPRGQIGELCVRGPNVMKGYWKLPAETAEAFFGEW
                     FRTGDLGTVDEDGYFSIVDRKKDMIIVNGMNVYPRIVEELLYRHPAVREAAVIGEPHE
                     LHGEVPVAFISLKEGASVDASALRAFCLESLGRHEVPRRVVFLPDLPKNAAGKIMKRA
                     LRKDGERERGVP"
     gene            complement(4579..5217)
                     /locus_tag="PK634_13540"
     CDS             complement(4579..5217)
                     /locus_tag="PK634_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98799.1"
                     /translation="MDLPLINEHLAALLAGKTIKTPHYDFKTGKRKLDVHEMSLKPNQ
                     ILMIDSLHGLYGEMTKSIEPNRKFKLYIETLGQVRTADGTFMRWADNRLLRRMIRDSW
                     HRNLQPLQTLTHWHYVRRSEMKNIVPYIGTVDYLVNSAQPFELPILRHKLFKFFPEAL
                     KLYKDDSKRQDAYLRAKRVHEFFTPLTAVTDDSVVPSKSLIREFIGGSEYHY"
BASE COUNT          953 a         1778 c         1728 g          797 t
ORIGIN      
        1 ggatgattgg tgatcggcga tcattgtcga ggttcccgag gacagcactg gatgtgggtg
       61 gcccgtgtgt tggaacgttc ggatgaatac aaaagaaggc gcacctgcct aagttgcaag
      121 gccgcgaact ccaagaagct gttccagatt cctcaaaaca gatggctttc gccatgctgt
      181 tggttgcgga attccaaaat gccagccaga tgggcaagca cagatttaaa gatccccgac
      241 caaaaaaaac acgccggcac gagccagccg cgtttgccca accccgggtt ggcgcagccc
      301 cggcgttgat acaattttca aggtggttcc agcgcctgtg ttgaagttgc gcgccgcacc
      361 cgcttgatta gggagcaaac tccagaccgt atatcatgct gcgggatacg cggcccggaa
      421 cagcaaccgg agttccgtgg gaacgaaccc gcgctcccgc agcgaggtcc ggccccccgc
      481 ttcacaatcc gatcgctccg tcagcgtcag cggatcaaat ccgcccgaag cgacatcgtc
      541 ctgcccgtgc cggcgtgcca tccggtatcc gttcagcacc gtgaacaggc accgggcgca
      601 ggggttcatg gacgcggaca cgttccgcag gatggccgcg ggctgaccca acgcgtcgtc
      661 cctggcgccc agcaggccga acgccccctc gcaaagacag atcgcggcat cgaaagagga
      721 gtcggtcgca aagcgcgtcg cgtccgtctc gatccacgtc accgcaaccc cggcggccgc
      781 ggctttctcc cgcgcctgcg acagcatgcc ggacgaaata tccacgccgg tcacccggaa
      841 gccacgccgc gccaactcga tggcgtggcg gcccgtcccg catccaacat ccagcaggct
      901 catccccggc cccagtccaa gttttcggat caggaaggct atctcgctct gcgtgttctg
      961 cgtaaagcag ttgccgtcgt actgcggcgc gtgcccgttg aaaaaaacgg cccactcgct
     1021 ggcggagcgc ttcacgaccg cgcggctcct tgccgattcc cggcccgcct cttttcccgc
     1081 gccttgacga tagagtagca gaaagaatcc cttggcgccg gccccagggc cgggcgaacg
     1141 atccacttcc tcaagacgcc ctcccgcgtc atgcctattt tctcaagcac gcgggccgag
     1201 gccttgttgt ccacgtcgca ggtcgcccaa acccggtaca ccgagggctg cctcatcgcc
     1261 caggccgtca gcgcccgggc ggcctcggtg gcgtatcccc ttccccacca ggcccggccg
     1321 agaacatagc cgatctcgat cctggacccg caggggcgaa tgccggcggc gccgatcagc
     1381 ccgccgcgct tgaggagcag ggcccataga tgctcggttc cctgctgcgc cgttatgcgg
     1441 gtctggcgca ggtatcggcg ggtctccgca aggctcttat gcgcccgcca ggccaggaag
     1501 cgcgtcgcct cgggatccga ggcataggct tcatgcacgg accgggcatc gcccgccctc
     1561 aaagcgcgca acaccatgcg acgcgtccga ataaccaggg gcgggttcat tcaaggcgcc
     1621 cactgtactt ccggactgcc gggccatgtc aagggagcag tcggaatgcg catcgcgcat
     1681 tattaaggaa aaaacgcgga atgctccgct cttctttcct ttctaatggc ggacaccagt
     1741 ccggagaagg gagggccccg aacggaacac gaacggacgg aacaatcgaa tagcggaaaa
     1801 tcctgcgggc cgccgccgcg gggcgcgcgc acgcgcccgg cccgacaagc ccgaccccgc
     1861 gcgggcatga aggactggcg caatgatccc ggcaagcgca gcgcgatgac gaggcggcgc
     1921 agcaacgcgc acgctcgccc gcgttgtctc ccgagtcgct ccgggttcga ggcggcattt
     1981 ctgcagcctt gctgcagaag cgctgtccgg ttggcgacgc aggacggcct gcgccgccac
     2041 gcaagatcgg aagcatctgc ccgccctgcg gaggcagcag agcggtggcc acgcgtaagc
     2101 cgtgcaaccg gatacagcaa ccgggaggcg gacttgatgt cggccgcatc atcgccctcg
     2161 cccggacgtg actgcgaaga cgcgccgccc gccgggaaac ggtcgcgttg cgggtccatt
     2221 catgccggcc cgcctgcagc gcacggcggc ccgcaccgct tgcgccgacg gactcgaggt
     2281 cctgacggca acccggcggc atccggcgcg gttcggcagg ttcagggccc gccggcgggg
     2341 gcacgcttac cagaagccgt agtggcccgc ggccaggacg tagaggccca gcaggaaatt
     2401 cgtgatcgca tgggccatgc ccgcagccca caggtcgcgg gtcttcacgg ccagcgcccc
     2461 gtaggcgagg ccggcgagga caccgaccag ccatcggtca tgctccaggc cgaacaggag
     2521 cgccgtgatg aaaaaagcgt tgcggacgaa cacgcccagg tccaccgacc ggaaatcgcg
     2581 agccgtgatc cagcggtaga tgaagccgcg ccagaagaac tcctcgatga acgaaatgac
     2641 gaaggccgag cccaggagtc ggacgatggc cagcggccag ccgcacgttt ccggcgcgta
     2701 gggcggcggc gaaaccgctt ccggcaggcg gccccaggga agcacggcgt atttcaggta
     2761 gaactcctga agggccggcc agcgcgccgc ccatccggtt tcggggagca cccagactgc
     2821 aaacaccagc acgcccaccc atgccgcggg gacgagattt ctcgcgcgca gcggcgcata
     2881 gccccgccag ggccgcaaac cgagaaacag gatcaggccc gcggccgccc gcacggcata
     2941 ggtccagccc gacggcgagc cggggaggta gatcagcgcc agccacgcgg caaagggaac
     3001 gacgtgggcc agcatatcct ggaggcgggg atcgcggctc acgggacgcc ccgctccctc
     3061 tctccgtctt ttcgcagcgc gcgcttcatg atcttgccgg ccgcgttttt cggcaggtcg
     3121 ggcaggaaga ccacccgacg cgggacctcg tgccgcccga ggctttccag gcagaaggcc
     3181 cgcagcgcgg acgcatcgac gctcgcgccc tccttgagcg aaatgaatgc gacgggaacc
     3241 tcgccgtgca gttcgtgcgg ctcgccgatc acggccgcct cgcgcacggc gggatggcgg
     3301 tagagcaact cctcgacgat gcggggataa acgttcatcc cgttgacgat gatcatgtcc
     3361 ttcttgcggt ccacgatgga gaagtagccg tcctcgtcca ccgtccccag gtcgccggtg
     3421 cggaaccatt cgccgaagaa ggcctcggcg gtctcggccg gcaatttcca gtagcccttc
     3481 atcacgttcg gcccgcggac gcacagttcg ccgatctgcc cgcgcggcaa ttcccggccc
     3541 tgctcgtcca tgatcttcat ctcgacgccg gggacgggca ggccgaccgt gcccggcttg
     3601 cgccgcccgc cgatcggatt gacgcaggtc acgggcgagc actccgtggg cccgtcgccc
     3661 tcgtagatcg ccttgccgaa acgctcttcg aactggcgca tgatctccac gggcagcgcg
     3721 gcgcccccgg agatggcgaa gcgcagggag gccagcttcc cggtgtattc cgccgggagc
     3781 cgcaggagca cgttgaacat gctcggcacg cccatgaaga tcgtcgcgcc gctcgccgcg
     3841 atggcgtcgg ccgcctgcgc cgggtcgaac ttcggaagcg gcacaatcat acagccggcg
     3901 aggagcggcg agagcatggc cgcggtcgcc gcgaaggcgt ggaacatcgg cagcacgacc
     3961 agcacgacgt cccgacccgg ctcgaaacgc agggcggaga ggacgctccg gacattgctc
     4021 acgaggttcc ggtgcgtcag catggcgccc ttcgggcggc cggtggttcc cgaggtatag
     4081 atgatcgcga ccaggtcctc cgccgggttc acgggcgggc acgggcaggc cccgccggcc
     4141 gagaggatcg aggcgaaggg catgtccccg agaggattct gggcgccgac gcaaacggca
     4201 aactcaaggc ttcgaacggc cggcttcagg ctccggacgc cctccgcgaa cgcctcgaaa
     4261 tagatcaggc cccgcgcgcc ggaatcgtcg aggatgaacg cgacttcctt cgggttcagc
     4321 agcaggttga ccggcacgac cacggcgccg gccttgacga tgccgaagta ggcgacggcg
     4381 aaggcgtcgg agttgatgca gtaaaggccg atgcggtcgc cgggcctcac cccgcgcgcg
     4441 gccagcgccg cggcggcccg gtcgctcgcg gcgtcgagcg cggcataggt cgtgacacgg
     4501 ccgccgaacg aaaccgcgac agcctccggg ctggcccggg cgacggcccg gaacgcctcg
     4561 acgaggctgc cggccccgtc agtagtggta ctcgctgcca ccgatgaact cccggatcag
     4621 ggacttcgac gggacgaccg agtcgtccgt taccgccgtc agcggcgtga agaactcgtg
     4681 gacgcgcttg gcgcgcaggt aggcgtcctg gcgcttggag tcgtccttgt agagcttcag
     4741 cgcctccggg aagaacttga atagcttgtg tctcaagatc ggcagctcga acggctgcgc
     4801 gctgttgacg aggtagtcca ccgtgccgat gtacgggacg atgttcttca tctcgctgcg
     4861 ccggacgtag tgccagtgcg tcagcgtctg cagcggctgg aggttccggt gccagctgtc
     4921 gcggatcatg cggcggagca gccggttgtc ggcccagcgc atgaacgtgc cgtccgccgt
     4981 ccggacctgg ccgagggtct cgatgtagag cttgaacttg cggttcggct cgatgctctt
     5041 ggtcatctcg ccgtacaggc cgtgcaggct gtcgatcatc aggatctggt tgggcttgag
     5101 gctcatctcg tgcacgtcga gcttccgctt gcccgtcttg aagtcgtagt gcggggtctt
     5161 gatggtcttg ccggccagca gggccgcgag gtgctcgttg atgagcggca ggtccatcaa
     5221 gaccccgcac tacgacttca agacgggcaa gcggaa
//
LOCUS       DAOOTK010000175         5254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9821_length_5254_cov_3.656857, whole genome shotgun sequence.
ACCESSION   DAOOTK010000175 DAOOTK010000000
VERSION     DAOOTK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9821_length_5254_cov_3.656857"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PK634_13545"
     CDS             complement(<1..1060)
                     /locus_tag="PK634_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOW98800.1"
                     /translation="MNPGNRIGGLAALLLGLALLSACEVRRPPRVVRMARPTISLHEA
                     VRLDNRDELALNRAVGADINARDGGGRTPLHIAAELGRVAAADLLISWGADVNAQDAH
                     GRSPMHAAAAAGHAGIERLLISASANVNATDTEGLTPVLVAHLQNRQDVFDLLIAAGA
                     EFDFAPEVEEWVPAPAPPAAVAAPAAPEPRRIQGSFRTWTSASAAQIEAEFVDIVTDM
                     VILRTPRDELLRIPITRLQREDQILARELSGAAKPRLVRRAPTPSEGGSTSAASLGPR
                     IGKQNSWTILENCHLLHNDSNDGDSVHISHQGKEYIFRLYYVDAPETSLNFPQRVEDQ
                     GRYFDLNTEEALGIGHKAA"
     gene            1255..3492
                     /gene="lptD"
                     /locus_tag="PK634_13550"
     CDS             1255..3492
                     /gene="lptD"
                     /locus_tag="PK634_13550"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOW98801.1"
                     /translation="MRRRIQSRWMGVLAAGWLAAAGAAAAEKQTATVGGVEIHADQLE
                     YESGKRLLTGRGHVVIVRLSDVLRADLVTYRTDTQDAVAQGNVILERAGSLWRGEKLK
                     YNFGTRLGDFGQFEYYTDPYYIRAQESRQVSSNEFVMKKAVFTTCEGERPLVFMKMRE
                     ARIIDDIKLRAKGVWIFLGPLPLLYTPFLSKRLDSMGWEFIPGRSGRSGYFLLSAYTF
                     PITTNLLSSTHIDYRSERGWAGGQDFIWGKAGSSPRGLVTAYYADDQKPFKNDEEEEE
                     WSEWVDSDRYRLRLSDIRSVTDRDYLVTELNYLSDPRVIEEFFNEEFRMRSQPENRIS
                     LIHRGDRFTASLAVNKRLNDFFGNINRLPEARLDVPRLQLGDTPFYYETYNGAAWLER
                     EYPEGQETNNYDSIRLDSSHMVYWPTRHFGFLNITPRGGYRATYYSTAYDVSYLTNQT
                     TVVESNVVIGITNSVERQVEERAVLRSLYETGVEVSFKAFKVLNEATLGRDYQGLRHV
                     VEPYAKHTYVPEPNIRPDELPQFDGVDELDRRHDVYFGVRNKLQTKRLAGGILPLQPA
                     ETGGAASLAAPSISGQRVHDLVDVGVFTTYRIERFDEVHDFSDIYFDARLRLVDWLPI
                     DTWVSYDPYESEVTEARVEASWLNLEDASRLSLQYLYRLDKRNQVAAELHLFPYDPWS
                     FRVYTRYNIDISELEEQGYFVEHKTSCLGIGTGYKRVEEDDDDQIWFQVWLTAFPKSY
                     IKMGL"
     gene            3514..4455
                     /locus_tag="PK634_13555"
     CDS             3514..4455
                     /locus_tag="PK634_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW98802.1"
                     /translation="MNAEYLVTGGGGFIGSNVVRTLVAQGRRVRVLDNLSTGRRENIG
                     DIEDRIDFVLGDIRDVETVRRAVQGVRYVLHAAALPSVARSVREPLASNDVNITGTLN
                     LLVAARDARVERLVYSSSSSVYGNTPVLPKREDMPPQPRSPYALTKLAGELYARLFTD
                     LYGLPTFSLRYFNVFGPRQDPSSQYSAVIPRFIDTLHHGQPLVIYGDGRQTRDFTFVD
                     DVVSENLCCCTAPLDAAGVPYNVAWGNRISLQELVAVLGRLMGKQVEPVFEPARAGDV
                     RDSQADSSQAMARLGWKPQVSFEEGLRKTVEWFLSHP"
     gene            4464..>5254
                     /locus_tag="PK634_13560"
     CDS             4464..>5254
                     /locus_tag="PK634_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOW98803.1"
                     /translation="MTPPASDLLLQTAVDAARAAGRHALQNRQRRREYVERSAHDVKL
                     ALDLECQRVAESVIARAWPDPRILGEEGGAGAGMDELVWVIDPIDGTVNFSHGMPLWC
                     CSVAVMQGGDTLAGAVFVPELDELYTAARDGVSERNGARIAVSDASSLAEAMILTGVS
                     KHMDSNPEALRFMATIASRAQKVRVMGAAAVDICHVACGRADGYFESSIYLWDVAAAG
                     LVARRAGGCAEILESLSPVHLRYLCTNGRIHEELKKLRTDFDKLR"
BASE COUNT          848 a         1727 c         1833 g          846 t
ORIGIN      
        1 gggcggcctt gtgcccgatg cccagcgcct cctcggtgtt gaggtcgaag tagcggccct
       61 ggtcctcgac gcgctggggg aagttcaggc tcgtctcggg cgcgtccacg taatagaggc
      121 ggaagatgta ttccttgccc tggtggctga tgtggaccga gtcgccgtcg ttggaatcgt
      181 tgtgcaggag atggcagttc tcgaggatgg tccagctgtt ctgtttcccg atccgcgggc
      241 cgagcgacgc cgcggacgtg gagccgccct cggacggggt gggggcacgc cgcaccagcc
      301 tgggtttcgc ggcgccgctc aattcccgcg ccaggatctg gtcctcgcgc tggagccggg
      361 tgatcgggat gcgcaggagt tcgtcccgcg gggtgcgcag gatgaccatg tccgtcacga
      421 tatccacgaa ttccgcctcg atctgcgcgg cggaggcgct cgtccaggtg cggaacgagc
      481 cctggatgcg gcggggttcc ggggccgccg gggcggcgac ggcggcgggg ggcgcgggcg
      541 cgggcaccca ttcctccacc tccggcgcga agtcgaactc ggcgccggcc gcgatcagga
      601 gatcgaatac gtcctgccgg ttctggaggt gcgcgaccag gacgggcgtc agcccttccg
      661 tgtccgtggc gttgacgttg gcgctggcgg agatcagcaa tcgttcgatg ccggcgtgcc
      721 cggcggcggc cgccgcgtgc atgggggagc ggccgtgcgc atcctgcgcg ttgacgtcgg
      781 cgccccagga gatcaggagg tccgccgcgg ccacccggcc cagttccgcc gcgatgtgca
      841 gcggggtgcg tccgccgccg tcccgcgcgt tgatatccgc gccgacggcg cggttgagcg
      901 ccagttcgtc gcggttgtcc agccgcacgg cctcgtggag ggagatggtc gggcgggcca
      961 tccggacgac ccggggcggc cggcgcacct cgcacgcgga gagcagggcg agccccagca
     1021 gcaacgccgc gagcccgccg atcctgttgc ccgggttcat ggatgcgctc cgaagttcac
     1081 ggtcgccagt ataggccaac ggccgggcat ggcaatccgg gagcgtgtat tccgctgcgt
     1141 ccggcctgcc cgcggggcgc cgcccgcgcg acgggccccc ttccgcggcg agaaactcct
     1201 tggctcgggc gggccggctg tgctagcgtg cgcccgattc aggtgcagcc aagaatgcgt
     1261 cgcagaatac agagccggtg gatgggcgtc ctcgcggcgg gatggctggc cgcggccggg
     1321 gcggccgccg ccgaaaagca aacggcgacg gtgggcggcg tggaaatcca tgccgaccag
     1381 ctcgaatacg agtccggcaa gcggctgctc actggccggg ggcatgtcgt gatcgtgcgt
     1441 ctctccgacg tgctgcgggc ggatctggtg acgtaccgga ccgacacgca ggacgcggtc
     1501 gcgcagggca acgtgatcct cgagcgggcc ggcagcctgt ggcgcggcga gaagctcaag
     1561 tacaacttcg gcacccgcct cggcgatttc gggcaattcg agtactacac cgatccgtac
     1621 tacatccgcg cgcaggagtc ccggcaggtc tcctcgaacg agttcgtgat gaagaaggcc
     1681 gtgttcacca cctgcgaggg ggagcggccg ctggtcttca tgaagatgcg ggaggcgcgg
     1741 atcatcgacg acatcaagct gcgcgccaag ggcgtctgga tcttcctggg gccgctgccg
     1801 ctcctgtaca cgccgttcct ctccaagcgc ctcgacagca tggggtggga gttcatcccc
     1861 ggccggagcg ggcggtccgg ctacttcctc ctctcggcgt acacgttccc gatcaccacc
     1921 aacctgctct cctcgacgca catcgactac cggtccgagc ggggatgggc cggcgggcag
     1981 gatttcatct gggggaaggc cggatcctcg ccccgtggcc tcgtgacggc ctactacgcc
     2041 gacgaccaga aaccgttcaa gaacgacgag gaagaggagg agtggagcga gtgggtggac
     2101 agcgaccggt accgcctgcg cctgtccgac atccggagcg tcaccgatcg ggactacctg
     2161 gtgaccgagc tgaactactt gagcgacccg cgggtgatcg aggagttctt caacgaggaa
     2221 ttccgcatgc gctcccaacc ggagaaccgg atctcgctga tccaccgcgg cgaccggttc
     2281 accgcctccc tcgcggtcaa caagcgactc aacgacttct tcgggaacat caaccggctg
     2341 cccgaggcgc ggctggacgt cccgcgcctg cagctcggcg acacgccgtt ctactacgag
     2401 acgtacaacg gcgcagcctg gctcgagcgg gagtatcccg aggggcagga gaccaacaac
     2461 tacgattcga tccggctgga ttcgagccac atggtctact ggccgacgcg ccacttcgga
     2521 ttcctcaaca tcacgccgcg gggcgggtac cgcgccacct actactcgac cgcctacgat
     2581 gtttcctacc tcacgaacca gaccaccgtg gtggagagca acgtggtgat cgggatcacc
     2641 aacagcgtgg agcggcaggt cgaggagcgg gccgtgctgc gcagcctgta cgagaccggc
     2701 gtggaggtgt ccttcaaggc gttcaaggtg ctgaacgagg ccaccctcgg gcgcgattac
     2761 cagggcctgc gccacgtggt cgagccgtac gccaagcaca cctacgtgcc cgagccgaac
     2821 atacggcccg acgaactgcc ccagttcgac ggcgtggacg aactggaccg gcggcacgac
     2881 gtctacttcg gggtccgcaa caagctgcaa accaagcggc tcgcgggcgg aatcctgccg
     2941 ctgcagccgg ccgagacggg cggggccgcc agcctcgcgg cgccgtcgat cagcgggcag
     3001 cgggtgcacg atctcgtgga cgtgggcgtt ttcaccacgt accggatcga gcggttcgac
     3061 gaggtgcacg atttcagcga catctacttc gacgcgcggc tgcggctcgt cgactggctg
     3121 ccgatcgaca cgtgggtgtc gtacgaccct tacgagagcg aagtcaccga ggcccgcgtg
     3181 gaggcctcct ggctgaacct ggaggacgcc agccggctgt ccctccagta cctctatcgc
     3241 ctggacaagc gcaaccaggt ggcggccgag ctgcacctgt tcccgtacga tccctggtcg
     3301 ttccgcgtct acacgcggta caacatcgac atcagcgaac tggaggagca gggctacttt
     3361 gtggagcaca agacctcgtg cctgggcatc gggacgggct acaagagggt ggaggaggat
     3421 gacgacgacc agatctggtt ccaggtctgg ctcacggcct tcccgaagag ctacatcaag
     3481 atgggccttt aatccggaaa gcatgacgaa gacatgaacg cggagtatct ggtgacgggc
     3541 ggcggggggt tcatcggctc gaacgtggtc cggaccctgg tggcgcaggg ccggagggtc
     3601 cgggtcctgg acaacctctc caccgggcgc cgggaaaaca tcggggacat cgaggaccgc
     3661 atcgatttcg tcctgggaga tatccgggat gtggagaccg tgcggcgggc cgtccagggc
     3721 gtccgatacg tgctgcatgc cgccgccctg ccgtccgtcg cccggtccgt ccgcgaaccc
     3781 ctggcgagca acgatgtcaa tatcaccggc acgctcaacc tgctcgtggc ggcgcgggat
     3841 gcccgcgtcg agcgcctggt gtactcctct tcctcctccg tgtacggcaa cacgccggtc
     3901 ctcccgaagc gggaggacat gccgccgcag ccccgctcgc cctatgccct gaccaagctc
     3961 gcgggcgagc tgtacgcgag gctttttacc gatctgtacg gcctgccgac gttctcgctg
     4021 cgttatttca acgtgttcgg cccgaggcag gatccgtcgt ctcaatattc cgccgtgatc
     4081 ccgcgcttca tcgacacgct gcatcacggg cagccgctgg tgatctacgg ggacggccgg
     4141 cagacgcggg acttcacctt cgtcgacgac gtggtctcgg agaacctctg ctgctgcacg
     4201 gctcccctgg acgcggccgg ggtgccgtac aacgtggcct ggggcaatcg catctcgctc
     4261 caggagttgg tcgccgtgct gggccggctc atgggcaaac aggtcgagcc cgtgttcgag
     4321 cccgcccgcg cgggtgacgt ccgggactcc caggcggatt cgtcccaggc gatggctcgc
     4381 ctcggctgga agccgcaggt atccttcgag gaaggcctgc ggaagaccgt cgagtggttc
     4441 ctctcccatc cgtaggcggc gccgtgacgc ctcccgcttc cgacctgctc ctgcaaacgg
     4501 ccgtggatgc cgcgcgcgcc gcgggacggc acgccctgca gaaccggcaa cggcgccggg
     4561 aatacgtgga gcgctcggcc cacgacgtga aactggccct ggacctggag tgccaacgcg
     4621 tcgccgagtc ggtcatcgcc cgcgcctggc cggatccccg cattctcggc gaggaaggcg
     4681 gagccggcgc cgggatggat gagctcgtgt gggtcatcga cccgatcgac gggacggtca
     4741 atttctcgca tggcatgccg ctgtggtgct gctccgtggc ggtcatgcag ggcggcgaca
     4801 cgctggccgg cgccgtcttc gtgccggagc tggatgaact gtacacggcg gcgcgggacg
     4861 gcgtatccga gcgcaacggc gcgcgcatcg ccgtttccga cgcgtcatcc ctggcggagg
     4921 ccatgatcct cacgggcgtg tccaagcaca tggattccaa cccggaggcg ctgcggttca
     4981 tggcgaccat cgcctcgcgg gcgcagaagg tgcgggtcat gggcgccgcc gccgtggaca
     5041 tctgccacgt ggcctgcgga agggcggacg ggtacttcga gtcgagcatc tacctctggg
     5101 acgtggcggc ggccgggctg gtcgcgcgcc gcgccggcgg ctgcgcggaa atcctggaat
     5161 ccttgagtcc cgtgcacctg cgttaccttt gcacgaacgg gcgcatccac gaagagctga
     5221 agaaactgcg aaccgacttt gacaagctgc ggga
//
LOCUS       DAOOTK010000176         5213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_9946_length_5213_cov_4.562233, whole genome shotgun sequence.
ACCESSION   DAOOTK010000176 DAOOTK010000000
VERSION     DAOOTK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_5213_cov_4.562233"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..625
                     /locus_tag="PK634_13565"
     CDS             335..625
                     /locus_tag="PK634_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98804.1"
                     /translation="MVKPAEQRVDIGGRRTQAGCGVSQSAAKLAVAVFFVAGILLNGH
                     HIYSEATRRPYGFWWTVWVNASKPLDYISSATRLSSARTWIENTFGQTEDTP"
     gene            622..1329
                     /locus_tag="PK634_13570"
     CDS             622..1329
                     /locus_tag="PK634_13570"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HOW98805.1"
                     /translation="MSKLLAALCAALAAWVATGLTAGAQATPQRVLIVGDSMMQIPAH
                     SLELALSRKPEVQTKAFTRIGSGLARLDVFDWLAQMRTLVDEFKPDTALVFLGANDHQ
                     AMKTGDTVIQPGEPAWEVEYARRVGEAMDILGAGKGTRVLWLQLPDMKEDKNREDADL
                     MNRIFAAEAAKHGVTFFETRPVLSRTPGKFAPYVIQANGMPLRVRSSDNIHLSREGAD
                     LLAVAVIASLWKETPNP"
     gene            1415..2857
                     /locus_tag="PK634_13575"
     CDS             1415..2857
                     /locus_tag="PK634_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOW98806.1"
                     /translation="MIFPTTDFAIFFLIVFFVSWALIRRHTGPWKLFMLAASWFFYGY
                     WDWRFIALLAGSSAFNHAMAVGIHRSRNPHVRRLLLTLGVAFNVASIGFFKYSRFLAE
                     QVVAVCGWMQFDLDAWFPVLDYLGQVILPVGISFFTFQAMSYVIDVYRRVMPPADSFI
                     DFALYLAFFPQLVAGPIVRACDLVPQISKPNPPPNIDTARAACLILGGLFKKIVVANY
                     LAQKIVDPVFARPDLYGAVDTLIGVYGYAIQIYCDFSAYSDIAIGLGLLLGFQINLNF
                     NAPYLAGSIQEFWRRWHISLSTFLRDYLFIPLGGSRGSELKTYRNLLITFLLGGLWHG
                     AGWTFIAWGALHGVYLSVERFFRRVMDRRGWQGEGLAVLWIKRIAVWHLVCLSWLFFR
                     GETFEHALTMLRAMKLWGLSATLVTGPVLLAIVVGYGMQWFDGDRAQRIWNAFGRLPV
                     VVQGIVTALILTVILALGPKGVAPFIYFQF"
     gene            2893..>5213
                     /locus_tag="PK634_13580"
     CDS             2893..>5213
                     /locus_tag="PK634_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOW98807.1"
                     /translation="MTLAALLGLWLAAGTAIAQEPPASPAEPAPELPAVPKLAIPDVP
                     PVTEPPLAVPELPTAEAPAAAAVEAVSPAAESVLPVPPAAPAEAAAEIPAVAPALEKA
                     VEAAVPEIPAADVAAPAVPSPAAAAAEALDTTPVASDLAPVAAPIAPGAAELEKNTPR
                     ARMYDAMLNVQDGKYEEAIPKLESVIADDPTLIDAWETLTWAYWLGGRPDDARQLAKE
                     FIGIAPDEPVAHNIMAQFATRDGELAEAERLYRRSLALKADQYETRFAFASVLLWNGK
                     FDEAMKELNDLYKQDPERLDVELQLARALTAFEEYEEALDHWDHINEMVPDYPEYMMA
                     RAEVLVLIGSLKEAQAEAERVLELEPDNLSALNLLADIAMRERKNDEVVKALKRVRDL
                     ADGDEAKAMVERRLAYFMKAEQDRDPATFTMDQVVQAGQDAYRRDPGDINGHLFYAEM
                     MTLDKDYGRAEEQFRNVLQEKNPHSDRALFGLFQCYLGRMMLDDAERQLRANLRDLNP
                     RDPYRHLMWAQLYFARGRFRDAMESLNRLEQEGAQGSIFTLQYNRLSSSEWTPYPSVR
                     QFRDHLMTLKRAGYRFISAGQFPSYFETCVRPERVQRRPFLYRVLRSISNAWTGEKDQ
                     SPPMLSDVVPERVACVTFDDGLRSTFRWATPLAEDLRIPMTVFVSLGHILSNDIRAAT
                     FPELKAHHDTGLWEINSHLVFATIASMTNRESRLVHQLPNLLWLEDKDRQETLREYYA
                     RLRYEFTESQRMLQKALGVETSQVASVAYPHGD"
BASE COUNT          820 a         1792 c         1770 g          831 t
ORIGIN      
        1 ccttcgaccc gctggaactc ggcggcataa tcggatttct gggggagggg gaatttctcg
       61 ccgctgggac tgacggaatg ctcggacatg gccgcctccg gtttggtcag ggtgtgtccc
      121 cgcctgttat gcccgaccat gctagggcga gggcggggcc tcgtcaatgc gaattgcctt
      181 tcccggcggg ccgggtcccg gtccgcccgc gtcggaaacg gcggcggatg ggcgcgccgg
      241 gcgccatatc ttgcttgcac cgcggcgcca tccgtgtttg actcgtgcca tcgaccatcg
      301 ggatgctcgt ggcgtactgt cagtggaggc ggccatggta aaaccggcgg agcaacgggt
      361 ggacatcggc gggcggcgca cccaggcggg ctgcggggtt tcccagtccg cggccaagct
      421 cgcggtggcc gtgttcttcg tggcggggat cctgctcaac ggccaccaca tctacagcga
      481 ggccacgcgc cggccctacg gcttctggtg gacggtctgg gtcaacgcct cgaaaccgct
      541 ggactacatt tcctcggcga cgcggctgtc ctcggcccgc acctggatcg aaaacacgtt
      601 cggccaaacg gaggataccc cgtgagtaag ctcttggcag cgttgtgcgc ggccctcgcc
      661 gcgtgggtgg cgaccgggct gacggccggg gcgcaggcga ccccgcagcg ggtgctgatc
      721 gtgggcgact ccatgatgca aatcccggcc cactcgctgg aactggcgtt gtcccggaag
      781 cccgaggtgc agaccaaggc gttcacccgc atcggctccg ggctggcccg gctggacgtc
      841 ttcgactggc tggcccagat gcggaccctg gttgacgagt tcaagcccga caccgccctg
      901 gtcttcctgg gggcgaacga ccaccaggcc atgaagacgg gggacaccgt gatccagccc
      961 ggcgagcccg cgtgggaggt cgagtacgcc cggcgcgtgg gcgaggccat ggacatcctg
     1021 ggcgcgggca agggcacgcg ggtcctctgg ctccaactgc cggacatgaa ggaagacaag
     1081 aaccgggaag acgccgacct gatgaaccgg atcttcgccg ccgaggcggc caagcacggg
     1141 gtgaccttct tcgagacccg cccggtgttg agccgcacgc cgggcaagtt cgcgccgtac
     1201 gtgatccagg ccaacggcat gccgctgcgc gtgcgcagtt ccgacaacat ccacttgagc
     1261 cgcgaggggg ccgacctgct ggcggtcgcc gtgatcgctt ccctttggaa agagaccccg
     1321 aatccatgag cccgcgggcg aaggcaaggg ccgtgtgggc gtcgagcggc ggtaacgagg
     1381 ccggacggcc cgcgccgcgg aggccggttg ccgcatgata ttccccacca ccgatttcgc
     1441 gatcttcttc ctcatcgtgt tcttcgtcag ctgggcgctg atccgccgcc acacgggccc
     1501 gtggaagctc ttcatgctgg cggcgagctg gttcttctac ggctactggg actggcgctt
     1561 catcgccctg ctggccggct cgagcgcctt caaccatgcc atggccgtcg gcatccaccg
     1621 gagccggaac ccccacgtgc ggcggctcct gctcacgctg ggcgtcgcgt tcaacgtggc
     1681 cagcatcggg ttcttcaagt actcgcggtt cctggcggag caggtggtgg ccgtgtgcgg
     1741 ctggatgcag ttcgacctcg acgcctggtt ccccgtcctc gactacctcg gccaggtgat
     1801 cctgcccgtc ggcatctcgt tcttcacgtt ccaggcgatg agctacgtga tcgacgtcta
     1861 ccgccgcgtg atgccgccgg cggacagctt catcgacttc gcgctgtacc tggccttctt
     1921 cccgcagctg gtcgcggggc ccatcgtccg cgcctgcgac ctggtgccgc agatcagcaa
     1981 gcccaacccg ccgccgaaca tcgacaccgc gcgcgcggcg tgcctcatcc tcggcggcct
     2041 gttcaagaag atcgtggtgg ccaactacct ggcgcagaag atcgtcgacc cggtgttcgc
     2101 ccggcccgat ctctacggcg ccgtggacac gctgatcggc gtctacggct acgcgatcca
     2161 gatctactgc gatttctcgg cctattccga catcgccatc gggctgggcc tcctgctggg
     2221 cttccagatc aacctgaact tcaacgcccc gtacctcgcc ggcagcatcc aggaattctg
     2281 gcggcgctgg cacatctcgc tctccacgtt cctgcgcgac tacctcttca tcccgctggg
     2341 cggctcgcgc ggctcggagc tgaagaccta ccgcaacctg ctgatcacgt tcctgctcgg
     2401 cgggctgtgg cacggggccg gctggacctt catcgcgtgg ggcgcgctgc acggcgtgta
     2461 cttgagcgtg gagcggttct tccggcgggt gatggaccgg cggggctggc agggcgaggg
     2521 gctggcggtc ctctggatca agcgcatcgc ggtatggcac ctggtctgcc tatcgtggct
     2581 gttcttccgc ggcgagacgt tcgagcacgc cctgacgatg ctgcgggcca tgaagctgtg
     2641 gggcctgtcc gcgaccctgg tcaccgggcc ggtcctcctg gccatcgtgg tggggtacgg
     2701 gatgcagtgg ttcgacgggg accgggcgca gcggatctgg aacgcctttg gccggctgcc
     2761 ggtcgtggtc cagggcatcg tgacggccct gatcctgacg gtgatcctgg cgctggggcc
     2821 gaagggcgtg gcgccgttca tctacttcca attctaagcg gctgaagaat aaaagtgcct
     2881 gaccagaaaa acatgacgct tgcggccctg ctgggcctgt ggctggccgc cggaactgcc
     2941 atcgcccagg agccgccggc atctcccgcc gaacccgcgc ccgaactgcc ggccgtgccg
     3001 aagctcgcga taccggatgt cccgcccgtc accgagccgc cgctcgccgt cccggaactc
     3061 ccgacggcgg aggctccggc cgcggccgcc gtggaagccg tctccccggc cgcggagtcg
     3121 gtcctgccgg tcccccccgc cgcgccggcg gaagccgcgg cggaaatccc ggcggtcgcg
     3181 ccggccttgg agaaggccgt ggaagcggct gtcccggaga tcccggccgc ggacgtggcc
     3241 gcgcccgcgg tcccgtcgcc ggcggccgcc gcggccgaag ccctcgacac gacgcccgtg
     3301 gcctccgacc tggcgccggt cgcggcgccc atcgcccccg gcgcggcgga gctggagaag
     3361 aacacgcccc gggcccggat gtacgacgcg atgctgaacg tccaggacgg aaagtacgag
     3421 gaggcgatcc cgaagctgga gtcggtgatc gccgacgacc cgacgctgat cgacgcgtgg
     3481 gagacgctga cgtgggccta ctggctcggc ggccggccgg acgacgcccg gcagctcgcg
     3541 aaggagttca tcggcatcgc cccggatgag ccggtggcgc acaacatcat ggcccagttc
     3601 gccacgcgcg acggcgaact ggccgaggcc gagcgcctct accgccggag cctggcgctg
     3661 aaggcggacc agtacgagac gcgcttcgcc ttcgccagcg tgctgctgtg gaacggcaag
     3721 ttcgacgagg cgatgaagga actcaacgac ctgtacaagc aggacccgga gcgcctggac
     3781 gtggaactgc agctggcgcg cgcgctgacg gccttcgagg aatacgagga ggcgctcgat
     3841 cactgggacc acatcaacga gatggtcccg gactaccccg agtacatgat ggcccgcgcg
     3901 gaggtcctgg tgctgatcgg ctcgctcaag gaggcccagg ccgaggccga gcgggtcctg
     3961 gagctggagc cggacaacct ctccgccctg aacctgctgg cggatatcgc catgcgcgag
     4021 cgcaagaacg acgaggtggt caaggcgctc aagcgggtgc gggacctcgc cgacggcgac
     4081 gaggccaagg cgatggtcga gcggcggctg gcctacttca tgaaggccga gcaagaccgg
     4141 gaccccgcga ccttcacgat ggaccaggtc gtgcaggcgg ggcaggatgc ctaccgccgc
     4201 gacccgggcg acatcaacgg gcatctcttc tacgcggaaa tgatgaccct cgacaaggac
     4261 tacggaaggg cggaggaaca gttccggaac gtgctgcagg agaagaatcc ccacagcgac
     4321 cgcgcgctgt tcggcctgtt ccagtgctac ctcgggcgca tgatgctcga cgacgccgag
     4381 cggcagctgc gcgccaacct gcgcgacctc aatccgcgcg acccgtaccg ccacctgatg
     4441 tgggcgcagc tctacttcgc ccgcgggcgg ttccgggacg cgatggagtc gctcaaccgc
     4501 ctcgaacagg aaggcgcgca gggctccatc ttcacgctgc agtacaaccg gctctcctcc
     4561 agcgagtgga cgccgtaccc gtccgtgcgc cagttccgcg accacctgat gaccctcaag
     4621 cgggcgggct accggttcat ctcggccggg cagttcccgt cctatttcga gacgtgcgtc
     4681 cgccccgagc gcgtccagcg ccggccgttc ctgtaccggg tcctccggtc gatcagcaat
     4741 gcctggacgg gggagaagga ccagtcgccg ccgatgctct ccgatgtcgt ccccgaacgg
     4801 gtggcctgcg tcaccttcga cgacgggctc cggtcgacct tccgctgggc gaccccgctg
     4861 gccgaggatc tgcgcatccc gatgaccgtg ttcgtgtcgc tgggccacat cctcagcaac
     4921 gacatccgcg cggccacctt cccggagctg aaggcgcacc acgacaccgg gctctgggag
     4981 atcaacagcc acctggtctt cgccaccatc gcgtcgatga ccaaccggga aagccggctc
     5041 gtccaccagc tgccgaacct gctctggttg gaggacaagg accggcagga gacgctgcgc
     5101 gagtactacg cgcggctccg ctacgaattc acggagagcc agcggatgct ccagaaggcg
     5161 ctcggcgtcg agaccagcca ggtcgccagc gtcgcctatc cccacggcga tat
//
LOCUS       DAOOTK010000177         5166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_10063_length_5166_cov_4.957934, whole genome shotgun sequence.
ACCESSION   DAOOTK010000177 DAOOTK010000000
VERSION     DAOOTK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10063_length_5166_cov_4.957934"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="ffh"
                     /locus_tag="PK634_13585"
     CDS             <1..1212
                     /gene="ffh"
                     /locus_tag="PK634_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOW98808.1"
                     /translation="EADVNYQVARDFISRVKEKAMGREVLESITPGQQMVKIVHDELV
                     ALLGGATRDFDLSGRPASVLLLGLHGAGKTTTAAKLARAWMKRGKSVLLVACDLRRPA
                     AVDQLRILAGQAGVAFCPPEPGEDLPVLGRRALDRAGREACDVVLFDTGGRSQMDAEL
                     VAEVRALRETVNPGNVVLVLDAAIGQESVHVAETFHKEVGLTGLILTKLDGDARGGAA
                     LSVHAVTGCPVLMTGTGERLDNLEPFHPDRMASRILGMGDVISLVEKAQQVVDQEEME
                     RMQERLLEDRFTLEDFLTQIQQVKKMGPMENLLEMMPGADMLRGRIRPEQAGKVTEDW
                     KRTEAIIRSMTPGERRNPDILNAGRRRRIAAGSGTTVQDVNTVLHQFEQARRMARQFK
                     HMQKRLIRKGR"
     gene            1288..1554
                     /gene="rpsP"
                     /locus_tag="PK634_13590"
     CDS             1288..1554
                     /gene="rpsP"
                     /locus_tag="PK634_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOW98809.1"
                     /translation="MAVKIRLRRMGSNKQPFFRVVVTDMRCPNSGRFLETVGWYDPKR
                     KGSNYELKVERIEYWTDKGAQVSDTVRSLLKKNRATQRRAAAPA"
     gene            1565..1795
                     /locus_tag="PK634_13595"
     CDS             1565..1795
                     /locus_tag="PK634_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOW98810.1"
                     /translation="MRELVEYIVRALVDHPQDVRLTEVDGERTVIFELRCHPEDVGKV
                     IGKNGKTVGAIRTLVSTAAARQGKRAMLEVVE"
     gene            1802..2518
                     /gene="trmD"
                     /locus_tag="PK634_13600"
     CDS             1802..2518
                     /gene="trmD"
                     /locus_tag="PK634_13600"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOW98811.1"
                     /translation="MGASLKIDILTIFPGMLEGFLGESMLKRAARSGAVEFRVVNLRD
                     FTNDAHRTTDDRPYGGGPGMVMKPEPIFAAVESLEPAGARVILMTPQGRRFDQAMARD
                     LAGESHLIFVCGHYEGVDERVRQALATDEISIGDYVLTNGAVATAVVVDAVVRLRPGV
                     LGGAGATEEESFSDGCLEYPQYTRPPEFRGMRVPDVLVSGDHEEIRRWRRSQSLQRTR
                     ERRPDLLGGGDDGLKKRGDG"
     gene            2515..2868
                     /gene="rplS"
                     /locus_tag="PK634_13605"
     CDS             2515..2868
                     /gene="rplS"
                     /locus_tag="PK634_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOW98812.1"
                     /translation="MRAKMLEKISLEQARKEAAPALHIGDAVRVQVKIKEGDKERLQA
                     FAGTLIARDGTGATETITVRRISYGEGVERVFPLHTPSVAKIEVEKHGKIRRAKLYYL
                     RRATGKKSRVTETAM"
     gene            2882..3517
                     /locus_tag="PK634_13610"
                     /pseudo
     CDS             2882..3517
                     /locus_tag="PK634_13610"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            3527..3955
                     /locus_tag="PK634_13615"
     CDS             3527..3955
                     /locus_tag="PK634_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOW98813.1"
                     /translation="MLWPFGKKKNGAEDARHRAGKWGEQQAERHLRGLGFGILARRFR
                     VGRRDEIDLIARDGATLVFVEVKTRASEEFGAPIASVKGGKRHALSRAAIRYMMQMRS
                     KPDYFRFDVVEVIGAEGRGTPEIRHTPNAFTLHPDYNVPW"
     gene            4022..5008
                     /locus_tag="PK634_13620"
     CDS             4022..5008
                     /locus_tag="PK634_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOW98814.1"
                     /translation="MLIPALAWPLAASAQLPDDLLEQAVQAGEQLAREHLPADLLDQY
                     EWPSPETWTEFWTGLTQTLETGSLDDLAWLKPAAESAIEMLRMVPEGASYADWLQQRM
                     DYFDMAEALVREVPAPGQPPPPPPPARRGELKTPPRPKPAPPPPAVQQRRMESARSTS
                     KWETKLSGRPAPARAAALAPRLKDIFRAEGVPPELIWLAEVESSFNPEARSPVGAAGL
                     FQFMPATAQRFGLSVKPKDERLAPEKSAGAAAKYLRYLHGMFHSWPLAFAAYNAGEGR
                     VGKLLKSAPARTFEAIQDRLPTETQMYVPKVLATIKLRERVDLSAPASSKSP"
BASE COUNT          870 a         1638 c         1887 g          771 t
ORIGIN      
        1 gaggccgacg tcaactacca ggtcgcccgc gatttcatca gcagggtcaa ggagaaggcc
       61 atgggccgcg aggtgctgga gagcatcacg cccggccagc agatggtcaa gatcgtgcac
      121 gacgaactgg tcgcgctgct gggcggcgcc acacgggatt tcgatttgtc cggccggccg
      181 gcttccgtgc tcctgctggg cctgcacggc gcgggaaaga cgaccacggc ggccaagctg
      241 gcgcgcgcct ggatgaagag aggcaagtcc gtcctgctgg tggcctgcga tctgcgccgg
      301 ccggccgccg tggaccagct ccgcatcctc gccggccagg ccggggtggc gttctgcccg
      361 ccggagccgg gcgaggactt gccggtcctg gggcggcggg ccctcgatcg ggccggccgc
      421 gaggcgtgcg acgttgtcct ttttgatacg ggcggccgct cccaaatgga tgccgaactg
      481 gtggccgagg tccgggccct gcgggaaacc gtgaacccgg gaaacgtcgt cctggtgctg
      541 gacgcggcca tcgggcagga atccgtccac gtggccgaga cgttccacaa agaggtcggg
      601 ctgaccggcc tgatcctgac gaaactcgac ggcgatgcgc gcggcggcgc ggccctgtcc
      661 gttcacgcgg ttaccggctg cccggtgctc atgaccggga cgggggagcg gctggacaac
      721 ctagagcctt ttcacccgga ccggatggcc tcccggatcc tggggatggg ggatgtcatc
      781 agcctggtgg aaaaggcgca acaggttgtg gatcaggagg aaatggagcg gatgcaggag
      841 cgcctcctgg aggaccggtt cacgctggag gatttcctga cgcagatcca gcaggtcaag
      901 aagatggggc ccatggagaa tctgcttgaa atgatgccgg gggctgatat gttgcgcggc
      961 cgcatccggc cggagcaggc cgggaaggtg accgaggact ggaagcggac cgaggccatc
     1021 atacgcagca tgaccccggg cgaacggcgg aatccggaca tcctgaacgc cggccgccgc
     1081 cggagaattg ccgcgggcag cgggaccacg gtgcaggacg tgaacaccgt gctccaccag
     1141 tttgaacagg cgcggcgcat ggcccggcag ttcaagcaca tgcaaaaaag gttgatccgg
     1201 aagggccggt agggtatcaa agccagtcac gttcagggcc gacgcccccc atgcgggcgg
     1261 cgccggcggg cgtgtcggga ggaagctatg gcagtcaaga ttcgattgcg gagaatgggc
     1321 agcaacaagc agcccttctt ccgggtggtg gtcacggaca tgcggtgtcc gaacagcggg
     1381 cgttttctgg aaaccgtcgg ctggtacgat ccgaagcgca agggctcgaa ctacgagctg
     1441 aaggtcgagc ggatcgagta ctggaccgac aagggcgcgc aggtgtccga cacggtccga
     1501 agcctgctga aaaagaaccg ggcgacacag cggcgggccg cggcgcccgc ttgaggatgg
     1561 ggttatgagg gaactggtgg agtatattgt ccgcgcgctg gtggatcatc cccaggacgt
     1621 ccgcctgacc gaggtggacg gcgagcgcac ggtgatcttc gagttgcgat gtcaccccga
     1681 ggacgtgggc aaggtaatcg ggaagaacgg caagaccgtc ggcgccatcc gcacgctggt
     1741 gagcacggcg gccgcccggc aggggaagcg ggccatgctg gaggtcgtcg agtgagcggg
     1801 catgggtgcg tcgctcaaga ttgacatcct gaccatattc ccggggatgc tggaagggtt
     1861 cctcggcgag agcatgctca agcgggccgc gcggtcgggc gcggtggagt tccgggtcgt
     1921 caacctgcgg gatttcacga acgacgcaca ccggaccacg gacgaccgcc cctacggggg
     1981 cgggccgggc atggtgatga agcccgagcc gatcttcgcc gcggtggagt cgctggagcc
     2041 ggcgggcgcg cgggtgatcc tgatgacgcc ccaggggcgg cggttcgacc aggcgatggc
     2101 gcgggacttg gccggcgaat cgcatctgat ctttgtatgc gggcattacg agggcgtgga
     2161 cgagcgcgtg cggcaggcgc tggccaccga tgagatttcc atcggcgact acgtgctgac
     2221 gaacggcgcg gtcgccacgg cggtcgtcgt ggacgccgtg gtgcggctgc ggccgggcgt
     2281 gctcggcggc gccggcgcga cggaggagga atccttcagc gacggctgtc tggaataccc
     2341 gcagtacacg cggcccccgg agttccgggg gatgagggtg cccgacgtgc tggtttcggg
     2401 cgaccacgag gaaatccgcc ggtggcggcg gagccagtcg ctgcagcgga cgcgcgaacg
     2461 ccgcccggac ctgctgggcg gcggggacga tggattgaaa aaacgaggtg acggatgaga
     2521 gcgaagatgc tggaaaaaat cagtctcgaa caggcccgga aggaagccgc gccggcgctg
     2581 cacatcggcg acgcggtgcg cgtccaggtg aaaatcaagg aaggcgacaa ggaacgcctc
     2641 caggcctttg ccggcaccct gatcgcccgc gacggcacgg gcgcgacgga gaccatcacc
     2701 gtgcggcgca tttcctacgg cgagggcgtg gagcgcgtgt tcccgctgca caccccgagc
     2761 gtggcgaaga tcgaggtcga gaagcacggc aagatccgac gcgccaagct ctactacctg
     2821 cgccgcgcca ccggcaagaa gtcgcgcgtg accgaaaccg cgatgtaagc ggaaggcgcg
     2881 cggcttgctg cgcttcgaac gtcaggcctg gtccgccggc tgcgcccgcg tggcgggggt
     2941 ggacgaggcc gggcgcggcc cgctggccgg gcccgtcgtc gccgcggccg tgattttcga
     3001 cccggctttt ctggtcgccc aggagcgcgg cgaactcgcc ggcctgaccg attccaagaa
     3061 gctgtccccc gccaagcgcg accatttctt cgccctgctg gcaaaccgcg ccgaactatc
     3121 gatcggcgtc ggccgggccg aggtcgagga gatcgaccgg ctcaatatcc tgcgcgccac
     3181 gcacctcgcc atggcccgag ccctgcgcgc gctccccggc cttccggacc atgccctggt
     3241 ggacgggctg cccgtgcccg ggctcccatg cccgtccacg gccgtcgtcg gcggggacgg
     3301 gttgagcctg tccatcgccg cggccagcgt cgtggccaag gtgacgcggg accgcctgat
     3361 gcaggaactg gatggcctcc atcccggcta tggcctggcg cggcacaagg ggtacggaac
     3421 gccggagcac ctcgaggccc tgcgccggct cggaccttcg ccctgccacc gccggtcttt
     3481 tgcgccggta tcccagctcg tattgccctt gtcatagcgt atttccatgc tctggccctt
     3541 tggaaaaaag aagaatggcg cggaagatgc ccggcatcgc gcgggcaagt ggggcgagca
     3601 gcaggccgag cggcacctgc ggggccttgg gttcggtatc ctggcgcggc ggttccgcgt
     3661 aggccgccgg gacgaaatcg atctcatcgc gcgggacggc gccacgctgg tcttcgtcga
     3721 ggtcaagacc cgcgcgagcg aggaattcgg cgcgcccatc gcgtcggtca agggcggcaa
     3781 gcgccatgcc ctgtcgcggg ccgccatccg gtacatgatg cagatgcgct ccaagcccga
     3841 ctacttccgc ttcgatgtcg tcgaggtgat cggcgcggag gggcggggga cgcccgaaat
     3901 acggcacacg cccaacgcct ttacgctgca tccggactac aacgtgccgt ggtgacctcc
     3961 gctcaattcg atgttgaata atccggcatc ttatcggtct aattcagcga tgaagcgcat
     4021 cgtgctcatt ccggctctgg cctggccgct ggccgcatcc gcccagctcc cggacgacct
     4081 gctggagcag gccgtgcagg ccggcgagca actggcccgg gagcatctgc ctgccgactt
     4141 gctggaccag tacgagtggc cttccccgga aacgtggacc gagttctgga ccggactcac
     4201 gcagacgttg gagacgggtt cgttggacga cctggcctgg ttgaagccgg ccgccgagtc
     4261 ggccatcgaa atgctgcgca tggtgccgga gggcgcgtcc tatgccgact ggctgcagca
     4321 gcgcatggac tacttcgaca tggccgaggc gctggtccga gaggtgcccg cgccgggtca
     4381 gccgccgcct cccccgccgc cggcccggcg cggcgaactg aagaccccgc cgcggccgaa
     4441 acccgcgccg ccgcccccgg ccgtgcagca gcggcgcatg gaaagcgcac gcagcacctc
     4501 caagtgggaa acgaaactgt ccggccgccc ggccccggcg cgcgcggcgg cgctggcgcc
     4561 gcggctgaag gatatctttc gcgccgaggg cgtgcccccg gaactgatct ggctggccga
     4621 ggtggagtcc tcgttcaatc ccgaggcgcg cagtccggtg ggcgcggcgg gattgttcca
     4681 gttcatgccg gccaccgcgc agcgcttcgg actgtccgtg aagccgaaag acgagcggct
     4741 ggcgccggag aagagcgcgg gggccgcggc gaagtacctc cggtatttgc acgggatgtt
     4801 ccattcctgg cccctggcct tcgcggccta caacgcgggg gaggggcggg tggggaagct
     4861 gctgaaatcg gcgcccgcgc ggacctttga agccatccag gatcgcttgc cgacggaaac
     4921 gcagatgtac gtgccgaagg tgctcgccac catcaagctt cgcgagcgcg tggatttgtc
     4981 cgcgcccgct tcgtcgaaga gcccgtagtt ctccgtgctg actcaggcgc gcggcgtgac
     5041 ggccgggtgc atgtagatgg gagtcggcgg agccgcgggc gctccatgcc ggtgccgggt
     5101 ttccgccagg gcgccgagcc atcgggcccg cgggaatcgg tcgctttcaa tccaccgcag
     5161 cgtgcg
//
LOCUS       DAOOTK010000178         5033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_10415_length_5033_cov_4.710727, whole genome shotgun sequence.
ACCESSION   DAOOTK010000178 DAOOTK010000000
VERSION     DAOOTK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10415_length_5033_cov_4.710727"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK634_13625"
     CDS             <1..427
                     /locus_tag="PK634_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW98815.1"
                     /translation="LVALLLGARGAHLSAWSAALPAIIAYHVLFNLSLALLVAAANVY
                     FRDTQHLVGLLMSAWFFVSPVMYNLSFVEKFAGPALTPAYLLNPAALIITAYRAAQVP
                     GALLPWSGAALVAWLWPAALLVLAWRLFQRAQRDFADWL"
     gene            424..1605
                     /locus_tag="PK634_13630"
     CDS             424..1605
                     /locus_tag="PK634_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW98816.1"
                     /translation="MSAIEAIQLGKAYRKQGIGPPTLFGALSRTLRGRRNEEFWALRD
                     VSFAVEKGRTVGVVGPNGSGKSSLLGLVSGTITPTRGAARTEGRVSSLLELGAGFHPD
                     LSGRENVFLNAALLGIPREDVRRRYDHIVAFAGLAEFMDMPVKHYSSGMYVRLGFAVA
                     TELNPDVLLVDEVLAVGDIAFQLKCLDRIRDFQRRGKTLLLVSHALQTVEEFCHEAFL
                     LYRGELVARGDPPDVILKYMTSFMGEGGYLYRQEFGTRDVEFTQVALRGPDGAESASF
                     QTGRALRVDIGYRAKARIENPVFGFSIKTGNGLYVFGSNTQIAKHPVPFIEGEGVMRL
                     TLDPLTLKGGNFFLSLSIHSADHATQYHRREDWYPFVVKDPTEAPGLFELHSSWRLGA
                     V"
     gene            complement(1624..2895)
                     /locus_tag="PK634_13635"
     CDS             complement(1624..2895)
                     /locus_tag="PK634_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98817.1"
                     /translation="MSGAALHKMGFLALLLGMAGGSAPGVEVILGEDRALVREVRTLA
                     LAQGLQTVVLDNLPSGIDLDTLTLRARRVDLELRGWALDGGTGVAAEASGEAVWPPAA
                     ASAPAGERPAGPVSVRAQVYAPDERRALEVEVAYRTSGLNWSAAYQVVVRGDRVEERE
                     PLSADLTGWVRIQNRAGVAFTNAAVRLVGRRVPEEEGRPPGFLMLDEVSPMSDLWRWR
                     PPEADIEYEYALPDPVDLPAGSAREFTLVKSARVPAARRYVLRAEEFSASLEAPPQPL
                     RKYIIVRNIAANRLGLPLPPGPVQLFLGSMRSQLLQTGDLPHTPVDGDIRIDLGPSPD
                     VTGRRGRQARAERPEGGEIETFAIEVLNGRDGAITVELDEKPPLILQWALVSSTRPCR
                     EVFRRLRYELDLPAGGRETVEYTVRVSRPSL"
     gene            complement(2908..4368)
                     /locus_tag="PK634_13640"
     CDS             complement(2908..4368)
                     /locus_tag="PK634_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOW98818.1"
                     /translation="MKAYAWVVLLAGCASAAAEGLPEVEASAPRGVILSIYDTGQTLV
                     SDLRSATLAKGDNRVRFGGLPALLDPASVSLGAVGGATELTLNEQSFVRPPEDLAGFL
                     ARYQGRPAEVAANGGISQGQLLAAAESSDRPAVLLQADDGSVSLFPDPAAIERIRVPS
                     AVPGPALTCLLSSPGEGPANLRMWYTTRGLAWEASYEMQLDEGAGEAELLLRAALHNR
                     SGGDFENARVRLVTTTQGRQSGAPGAASRLPGEEVTTPFRYARGLETPVPESSLAPTA
                     QSALDVPGVVALPSGGARYVQLARAAKLPVRRFYIYDGVRFDRFQRNRRTDWNFGTEF
                     HREVDLRIGFDNTEAAGLGFVLPPGRLRLYTRRADGTADGLVEGRLAALDPGASADVS
                     LGPARGLSGERERTGYNEIVPLREYEESFEIRLANRSADEVEIRVVEHVYRGGQFEIV
                     KSDADYTMTGEQTLEFRPTLKPGGDKSIHYTVRYRW"
     gene            4513..>5033
                     /locus_tag="PK634_13645"
     CDS             4513..>5033
                     /locus_tag="PK634_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOW98819.1"
                     /translation="MEMTGLGTTGLRVSRLAMGTMTFGKEADRATSAALFHRAREAGI
                     NLFDCADIYALGESEKILGELIRGVRDQVVITSKVYFAMEPGLKRAGLSREHILRSAE
                     GSLKRLKTDRLDIYFAHAHDDLVPIEETLGALDELVRQGKALHTGASNFAAWQVARAL
                     GISDVNRWTRFSC"
BASE COUNT          760 a         1778 c         1721 g          774 t
ORIGIN      
        1 cctcgtcgcc ctcctcctcg gcgcgcgggg cgcgcatctc tccgcctgga gcgccgcgct
       61 gcccgcgatc atcgcgtatc acgtcctgtt caacctgtcc ctggccctgc tcgtcgcggc
      121 ggccaacgtg tacttccggg acacgcagca tctcgtgggg ctgctgatga gcgcgtggtt
      181 tttcgtcagc ccggtgatgt ataacctctc gttcgtcgag aagttcgccg gcccggcgct
      241 gacccccgcc tacctgctca accccgcggc cctgatcatc accgcctacc gcgccgcgca
      301 ggtgccgggc gcgctgctcc cgtggtcggg cgccgcgctg gtcgcctggc tctggccggc
      361 ggccctgctg gtcctggcgt ggcggctctt ccagcgggcg cagcgggatt tcgcggactg
      421 gctatgagcg cgatcgaagc cattcaactc ggaaaggcct accgcaagca gggcatcggc
      481 ccgccgaccc tcttcggggc cctgtcgcgg acgctgcgcg gacggcgcaa cgaggagttc
      541 tgggcgctgc gggatgtcag cttcgcggtg gaaaaaggcc gcaccgtggg ggtggtcggg
      601 cccaacggct cgggcaagag ctccctgctc ggactggtct cgggcacgat cacgccgacg
      661 cgcggcgcgg cgcggaccga ggggcgggtc tcgtcgctgc tggagctcgg cgcgggattc
      721 caccccgacc tgtcgggccg ggagaacgtt ttcctcaacg cggcgctgct gggcatcccg
      781 cgcgaggacg tccggcgccg ctacgaccat atcgtcgcct tcgccggcct ggccgagttc
      841 atggacatgc cggtgaagca ttactccagc ggcatgtacg tgcggctggg cttcgccgtg
      901 gccacggagc tgaacccgga cgtgctgctg gtggatgagg tgctggccgt cggcgacatc
      961 gccttccagc tgaaatgcct cgaccggatc cgcgatttcc agcggcgcgg caagacgctg
     1021 ctgctggtct cccacgccct ccagaccgtc gaggaattct gccacgaagc gttcctgctc
     1081 taccgcggcg agctcgtggc ccgcggggac ccgccggacg tgatcctgaa atacatgacc
     1141 tcgttcatgg gcgaaggagg ctatctctac cgccaggagt tcggcacgcg ggacgtggag
     1201 ttcacgcagg tggcgctgcg cggacccgac ggggcggagt cggccagctt ccagaccggg
     1261 cgcgccctgc gcgtggacat cggctaccgc gcaaaagccc ggatcgagaa ccccgtgttc
     1321 ggcttttcga tcaagaccgg caacgggctg tacgtgttcg gaagcaacac gcagatcgcg
     1381 aagcatcccg tgccgttcat cgagggcgag ggcgtcatgc ggctcacgct ggacccgctg
     1441 accctgaagg gcggcaattt cttcctgtcg ctgtccatcc actcggccga ccacgccacg
     1501 cagtatcacc ggcgcgagga ctggtacccg ttcgtcgtga aggatccgac ggaggcgccg
     1561 ggcctgttcg agctgcacag ctcgtggcgc ctcggggccg tgtgaccacg ccgccgggcc
     1621 tgttcacagg ctcggccggc tgacgcgcac ggtgtactcc acggtctccc ggccgccggc
     1681 cggcaggtcc aactcgtacc gcaaccggcg gaacacttcg cggcacggcc gcgtggaact
     1741 gacgagggcc cactggagga tgagcggcgg cttctcgtcc agctccaccg tgatcgcgcc
     1801 gtcgcgcccg ttcagaacct cgatcgcaaa ggtttcgatc tccccgcctt ccggccgttc
     1861 ggcccgggcc tgccggcccc gccgccccgt gacatccggg gaaggtccca ggtcgatccg
     1921 gatatcgccg tccacgggcg tgtgcggcag gtcgcccgtc tgcaggagct gggaccgcat
     1981 gctgccgaga aacagctgca cggggccggg cggcaggggg aggccaagcc ggttggcggc
     2041 gatgttgcgc acgatgatgt atttccggag cggctgcggc ggggcctcca gggaggccga
     2101 gaattcctcc gcgcgcagga catagcgcct cgcggcgggc acgcgggcgc ttttgaccaa
     2161 ggtgaactcg cgggcggacc cggccggaag atcgacggga tccggcagcg cgtattcgta
     2221 ctcgatgtcg gcttccggcg ggcgccagcg ccagaggtcg gacatgggac tgacctcgtc
     2281 gagcatgagg aagccgggcg gcctcccctc ctcttctgga acgcggcggc cgaccaggcg
     2341 gaccgcggcg ttcgtaaagg cgacccccgc gcggttctga atgcgcaccc agccggtcag
     2401 atcggccgac aggggctcgc gctcctccac gcggtcgccg cggacgacca cctgataagc
     2461 cgccgaccaa ttcaagcccg aggtccggta cgcgacttcg acctccaggg cgcggcgttc
     2521 gtccggagcg tagacctgcg cccgcacgga aaccgggccg gcaggacgtt ccccggccgg
     2581 cgcggatgca gccgcaggcg gccagaccgc ttcgccggag gcctccgcgg cgacccctgt
     2641 cccgccgtca agcgcccagc cccttaattc cagatccacg cgccgcgccc gcaacgtcaa
     2701 cgtatcgagg tcgatcccgg agggcaggtt gtccagcacc acggtttgca ggccctgcgc
     2761 gagcgcaagc gtgcggacct cgcgcaccag cgcgcgatcc tcgccgagga tcacctcgac
     2821 gccgggcgcg ctcccgccgg ccatcccgag caggagggcc aggaagccca tcttatgcaa
     2881 cgcggcgccg gacatgatag gccccgttca ccagcggtag cgcacggtgt aatggatgga
     2941 cttgtccccg cccggtttga gcgtcgggcg gaattcaagg gtctgttcgc cggtcatggt
     3001 gtagtccgcg tcggatttca cgatctcgaa ctgcccgccc cggtacacgt gctcgacgac
     3061 gcgtatttcc acctcgtccg ccgaacggtt ggccagccgg atctcaaagc tttcctcgta
     3121 ttcgcgcagc ggcacgattt cgttgtagcc cgtgcgctcg cgctcgccgc tcaagccccg
     3181 cgccgggccg agggacacat cggcggaggc cccgggatca agggcggcca ggcgtccttc
     3241 gaccagcccg tccgcggttc cgtccgctcg ccgggtgtag agccggagcc ggccgggcgg
     3301 caggacaaag cccagcccgg cggcctcggt attgtcaaat ccgatgcgca ggtccacctc
     3361 gcgatggaac tcggtgccga aattccagtc ggtgcgccgg ttccgctgga atcggtcgaa
     3421 gcgcacgccg tcgtagatgt agaaacggcg gacgggcagc ttggccgccc tcgccaattg
     3481 cacgtagcgg gcgccgccgg acgggagggc cacgacgccc gggacatcca gcgccgactg
     3541 ggccgtcggg gccagggacg attcgggcac aggggtctcc aggccgcggg cgtagcggaa
     3601 gggagtcgtg acctcctcgc ccggcagccg gcttgccgcg ccgggcgcgc ccgactgccg
     3661 gccctgcgtc gtcgtcacca ggcggacgcg cgcattttca aaatcgcccc cgctccggtt
     3721 gtgcagggct gcgcgcagca gcagttcggc ctcgccggcg ccctcgtcca actgcatttc
     3781 gtaggaggcc tcccaggcca ggccccgggt ggtgtaccac atgcggaggt tggcggggcc
     3841 ttcccccgga ctggacagca ggcaggtgag cgcgggcccc gggacggccg acggcacacg
     3901 gatccgctcg atggcggcgg gatccgggaa caggctcacc gacccgtcgt cggcctgcag
     3961 cagcaccgca ggccgatcag aggattcggc cgcggccagc agttgcccct ggctgattcc
     4021 tccgttcgcg gcgacttccg ccggccggcc ctggtaccgg gcaaggaagc ccgcgagatc
     4081 ctcggggggg cgaacgaacg actgctcgtt cagcgtcaat tccgtcgccc cgcccacggc
     4141 gcccagggac acgctggccg ggtccagcag cgccggcagc ccgccgaagc ggacccggtt
     4201 atcgcccttg gccagcgtgg ccgaccgcag gtccgaaacc agggtctggc cggtatcgta
     4261 aatggaaagg atcacccccc gcggcgcgga ggcctcgacc tcgggcaggc cttccgcggc
     4321 cgccgacgcg catccggcga ggagcaccac ccatgcatat gctttcatcg cgcttccctt
     4381 tccttgcccg cgaggcgcgg gcatggagtg aactgtacac cgttcttccc gggcctgtca
     4441 cgccccgcgg gttttgctct tgccggatcg accgcccgac ctatgctggc gccattcaca
     4501 aaggaagccc tcatggaaat gaccggactg gggacgaccg gcctgcgcgt atcgcgcctg
     4561 gccatgggca cgatgacatt cggcaaggag gctgaccgcg cgacctccgc cgccctcttc
     4621 caccgcgccc gcgaggcggg catcaacctg ttcgactgcg cggacatcta cgcgctgggc
     4681 gaatccgaga agatcctggg cgaattgatc cgcggcgtcc gcgaccaggt cgtgatcacc
     4741 agcaaggtct atttcgccat ggagccgggc ctgaagaggg ccgggctgtc gcgggaacac
     4801 atcctgcgct ccgccgaggg cagcctgaag cggctgaaga ccgaccgcct ggacatctac
     4861 ttcgctcatg cgcacgacga tctcgtgccg atagaggaga cgctgggcgc gctggacgaa
     4921 ctggtccggc agggaaaggc gctccacacg ggcgcgagca atttcgcggc ctggcaggtc
     4981 gcccgcgcgc tcggaatttc cgacgtgaac cgctggacgc gcttcagctg cat
//
LOCUS       DAOOTK010000179         4914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_10740_length_4914_cov_5.800370, whole genome shotgun sequence.
ACCESSION   DAOOTK010000179 DAOOTK010000000
VERSION     DAOOTK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_4914_cov_5.800370"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1420
                     /locus_tag="PK634_13650"
     CDS             62..1420
                     /locus_tag="PK634_13650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOW98820.1"
                     /translation="MEALASLCKRRGFIFQSSEIYGGINGFWDYGPLGVELKRNIKES
                     WWRAMVQNRDDIVGLDSSIIMHPRIWEASGHVKGFTDPMVDCRKCKGRWRADQLPETK
                     CPQGGPHEYTEARLFNLMFETNVGPVKDASTVAYLRPETAQGIFAQFKNVLEVARQKV
                     PFGIAQIGKAFRNEINPRNYTFRSREFEQMELEFFIKPGTDAEWHEYWVAERLKWYQS
                     IGLPEGHLHRYVYPKEELAHYASACVDVMYDFPFGTQELEGIAARGNFDLSRHQEFSG
                     KSMEYFDEETKQKFVPHVVEPSAGVDRIALALLCEAYHEEWVPTAEAAGQPPQPTEPG
                     QPPPQGYEARTVMRFAPRAAPVKVAVFPLMKNKPELVEKARGVFDTLRRRWPAFWDVA
                     GAIGRRYRRQDEIGTPFCITVDFQTLEDTTVTIRDRDTMKQERVAIADLPAKLAATLD
                     AA"
     gene            1417..2163
                     /gene="rph"
                     /locus_tag="PK634_13655"
     CDS             1417..2163
                     /gene="rph"
                     /locus_tag="PK634_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOW98821.1"
                     /translation="MTAPAKKTARPDGRAPDALRPIRFTPDLAPAASGSVLVEFGRTR
                     VICGASIEDGVPRWMRAQNVSGGWLTAEYSLLPYATKPRSSRESSTGKVSGRTQEIQR
                     LIGRSLRAVTDLDKLGERTIWLDCDVLEADGGTRTAAVTGAWVALRRAVNKLLKDGAL
                     KEDPLREAVAAVSVGIVEGRPLLDLCYTEDLAAAVDMNVVMTASGRFVEVQGTAEETP
                     YSREELDGLLDLAAKGIRQLLKAQRLVIRG"
     gene            2230..3000
                     /gene="xth"
                     /locus_tag="PK634_13660"
     CDS             2230..3000
                     /gene="xth"
                     /locus_tag="PK634_13660"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOW98822.1"
                     /translation="MKIATFNANSIRSRLDVVLAWLKQHQPDILAVQETKVVDELFPR
                     EPIEAAGYRVAFRGQKAYNGVAIISKKPADEVRFGFDDKGPADETRLLHARFGKLHVV
                     NTYVPQGREVDHPMYRYKIEWYARLRKYFDRHFTTRDRVIWLGDLNVARTPLDVHNPE
                     ERENHVCYHHAAREAFEQCLAWGFTDVFRKHHPEPGQYSFFDYRTPRAVDRGLGWRLD
                     YILASPPLAKGSRDARIDLDPRRANPSSDHTFVFADIS"
     gene            3078..3731
                     /locus_tag="PK634_13665"
     CDS             3078..3731
                     /locus_tag="PK634_13665"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOW98823.1"
                     /translation="MNTEHALNKEDAKKRILLAAMEKFADRGFGPTSVREICEAAGVN
                     VAAVNYYFRSKEQLYHEVFKTLFEDVGAPLMAIPAKVHDQASWKAALMEWIMTALRLV
                     TEEKPPTRWMVQLTAHERTHPSSMFPVLYESFFKPLRESLGMLLRMGMPAGTDETDTC
                     IWEITVASQVVVYTHRAPPWYALLIPPDVPREEWIERTARIIAGGITSRLAYQTGLS"
     gene            3728..>4914
                     /locus_tag="PK634_13670"
     CDS             3728..>4914
                     /locus_tag="PK634_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOW98824.1"
                     /translation="MKPSHLFLLRRVLSSTLLAAFALSSGCARFEPARARREQTESFT
                     NRLARLAKIELAKPLSLDDCIGIAMTNNYEIRKVDLDRELYRIGKNVAFTAFLPNVAA
                     SAGYSAYSKIPQNSEKEFDTIGVDIGMPIFMPSTWFLYAAARHGYASAGIAAHYVRQG
                     IVLQTSINYFNVLVQQDTVAALESQWTAARETAERVQGLADEGFIIQWEADQALLAVD
                     TRRAELNRARRRMAVLRGELLQGMGLSPAADITLSGEAHGAERPEGSVEDLVLKALEI
                     HPELAMADRQVVIQDHRVRQAFTAFLPTISLFSSGEWTGNDLAAHAENWVSGFQGAWT
                     LFDGLANVARYKAAKVERRKTQIERENTFLSIMVGVVAAEAAVRDAAEGARVRRRAYD
                     VAAA"
BASE COUNT          889 a         1712 c         1548 g          765 t
ORIGIN      
        1 ccggccccgc ctgctggcgc accatgccgc tcccatgaca acggacaaag cccaactgac
       61 catggaagcc ctggcctcgc tctgcaagcg ccgcggcttc attttccaga gctccgaaat
      121 ctacggcggc atcaacggct tctgggacta cggcccgctc ggcgtggagc tcaagcggaa
      181 catcaaggag tcctggtggc gcgccatggt gcagaaccga gacgacatcg tcggcctcga
      241 ctcctcgatc atcatgcacc cgcgcatctg ggaggcctcc ggtcacgtga agggcttcac
      301 cgatcccatg gtggattgca ggaagtgcaa gggccgctgg cgcgcggacc agctgcccga
      361 gaccaagtgc ccgcagggcg gcccgcacga gtacaccgag gcgcgcctct tcaacctgat
      421 gttcgagacc aacgtcgggc cggtcaagga cgcctcgacg gtggcctacc tccgccccga
      481 gaccgctcag ggcatcttcg cccagttcaa gaacgtgctg gaagtcgccc gccagaaggt
      541 cccgttcggc atcgcgcaga tcgggaaggc gttccgcaac gagatcaacc cgcgcaacta
      601 caccttccgc tcgcgcgagt tcgagcagat ggagctggag ttcttcatca agcccggcac
      661 ggacgccgag tggcacgaat actgggtcgc ggagcggctg aagtggtacc agtccatcgg
      721 cctgcccgag ggccacctgc accgctacgt ctaccccaag gaggaactcg cccactacgc
      781 cagcgcctgc gtggacgtga tgtacgactt cccgttcggc acgcaggagc tcgagggcat
      841 cgccgcgcgc ggcaacttcg acttgagccg ccaccaggaa ttcagcggca agtccatgga
      901 atacttcgac gaggagacga agcagaaatt cgtcccgcac gtcgtcgagc cgtccgccgg
      961 cgtggaccgc atcgcgctcg cgctgctctg cgaggcctac cacgaggaat gggtgcccac
     1021 cgccgaggcc gccggccagc ccccgcagcc gaccgagccg ggccagccgc cgccccaggg
     1081 ctacgaggcc cgcacggtta tgcgcttcgc cccgcgcgcg gcgccggtca aggtcgccgt
     1141 gttcccgctg atgaagaaca agccggagct ggtggaaaag gcgcgcggcg tgttcgacac
     1201 gctgcgacgc cgctggcccg cgttctggga cgtcgccggc gccatcggcc gccgctaccg
     1261 ccgccaggac gagatcggca cgcccttctg catcaccgtg gacttccaga ccctcgagga
     1321 caccacggtc accatccgcg accgcgacac gatgaaacag gaacgcgtcg ccatcgccga
     1381 cctgcccgcg aagctcgcgg cgacgctgga cgctgcatga ccgcgcccgc gaagaagacc
     1441 gcgcgccccg acggccgcgc accggacgcc ctgcggccca tccggttcac cccggacctg
     1501 gccccggccg cgtcgggctc cgtgctcgtc gagttcggcc gcacgcgggt gatctgcggc
     1561 gcgagcatcg aggacggcgt gccgcgctgg atgcgcgcgc agaacgtgtc cggcggctgg
     1621 ctcacggccg agtacagcct cctcccctac gccaccaagc cccgtagctc gcgggagagc
     1681 agcacgggca aggtcagcgg ccgcacgcag gaaatccagc gcctgatcgg acgcagcctg
     1741 cgcgccgtaa ccgacctcga taagctgggc gaacgtacga tctggctcga ttgcgacgtg
     1801 ctcgaggccg acggcggcac gcggacggcc gcggtcaccg gcgcctgggt cgcgctgcgg
     1861 cgcgcggtca acaagctcct gaaggacggc gcgctcaagg aggaccccct gcgcgaggcc
     1921 gtggccgcgg tcagcgtcgg gatcgtcgag ggccggccgc tgctcgacct ctgctacacg
     1981 gaggacctcg ccgccgccgt ggacatgaac gtggtcatga ccgcctccgg gcggttcgtc
     2041 gaggtccagg gcaccgccga ggaaaccccg tattcccgcg aggaattgga cggcctgctc
     2101 gatctggcgg ccaagggcat ccggcaactc cttaaagcgc aacggcttgt catcaggggg
     2161 tagggcggct tggcccaagc cgccggaaga aagcggcgcg ttgggccaac gcgccctacc
     2221 cataggctca tgaagatcgc caccttcaac gccaactcca tccgctcgcg cctggacgtg
     2281 gtcctcgcct ggctgaagca acaccaaccg gacatcctcg ccgtccagga aaccaaggtc
     2341 gtggacgaac tcttcccgcg cgagcccatc gaggccgcgg gataccgcgt cgcgttccgg
     2401 ggccagaagg cctacaacgg cgtcgcgatc atttccaaaa agccggcgga cgaagtgcgc
     2461 ttcggcttcg acgacaaggg cccggcggac gagacccgcc tgctccacgc gcgcttcggg
     2521 aagctgcacg tggtcaacac ctacgtcccg caggggcgcg aggtggatca cccgatgtac
     2581 cggtataaga tcgagtggta cgcccggctg cggaaatact tcgaccggca tttcacaacc
     2641 cgcgaccgcg tgatctggct cggggatctc aacgtcgcgc gcaccccgct ggacgtccac
     2701 aacccggagg aacgggaaaa ccacgtttgc tatcaccacg ccgcgcgcga ggcgttcgag
     2761 cagtgcctcg cgtggggctt cacggacgtc ttccgcaagc accatcccga accgggccag
     2821 tactcgttct tcgactaccg caccccgcgc gcagtagacc ggggtctggg ctggagactg
     2881 gactacatcc tcgcctcgcc gccgctggcc aagggcagcc gggatgcccg catcgacctc
     2941 gatccgcgcc gggccaatcc gtcctccgac cataccttcg tgttcgccga tatttcttag
     3001 cgcccccctt gacaagaatc acgacttggt atatcagtta aaaaaatcgt tttatacatt
     3061 tgtttaatac ggcgcacatg aacacggaac atgctttaaa taaagaagat gcgaagaaac
     3121 gcattcttct ggccgcgatg gaaaagttcg ccgatcgtgg cttcgggccg accagtgtgc
     3181 gcgaaatctg cgaggcggcc ggggttaatg tcgccgccgt taattactac ttccgttcga
     3241 aggagcagtt gtatcacgag gtgttcaaga cgctcttcga ggacgtcggc gcgccgctga
     3301 tggccatccc ggccaaagtc catgaccagg cctcgtggaa ggccgccctg atggaatgga
     3361 tcatgacggc gctccggctc gttaccgagg agaagccgcc gacccgctgg atggtccagc
     3421 ttacggccca cgagcgcacc catccatcga gtatgtttcc cgtcttgtac gaaagtttct
     3481 tcaagccgct gcgcgagagc ctgggcatgc tgctgcggat gggcatgccg gccggcaccg
     3541 acgaaacgga tacgtgcatc tgggagatca ccgtggcctc gcaggtggtt gtctatactc
     3601 accgggcccc gccgtggtat gccctcctga tcccgccgga cgtgccgcgc gaggagtgga
     3661 tcgagcgcac ggcgcggatc atcgccgggg gcatcacgtc ccggcttgcc tatcaaacag
     3721 gattatcatg aaacccagcc atcttttcct tcttcggaga gttctgtctt cgacgctgct
     3781 ggcggcgttc gcgctgtcgt ccggctgtgc ccgcttcgag ccggcgcgcg cccgccgcga
     3841 gcagaccgaa tccttcacga accgtctcgc ccgtctcgcc aaaatcgaac tggccaagcc
     3901 gctgtcgctg gacgattgca tcggcatcgc catgaccaac aactacgaga tccgcaaggt
     3961 cgacctcgac cgcgagctgt accgcatcgg aaagaacgtc gcgttcacgg cgttcctgcc
     4021 gaacgtcgcg gcctcggcgg gttacagcgc gtactccaag atcccgcaaa acagcgagaa
     4081 ggaattcgac accatcggcg tggacatcgg catgccgatc ttcatgccgt ccacctggtt
     4141 cctttatgcg gcggcgcgcc acgggtacgc ctccgcgggc atcgccgccc actatgtccg
     4201 ccagggcatc gtgctgcaga ccagcatcaa ctatttcaac gttctggttc agcaggacac
     4261 cgtggccgcg ttggaaagcc agtggacggc ggcgcgcgag acggccgagc gcgtccaggg
     4321 cctcgcggac gaagggttca ttatccagtg ggaagcggac caggccctcc tggccgtcga
     4381 cacgcgccgg gccgagctca accgcgcccg ccgccggatg gccgtgctcc gcggcgagct
     4441 gctccagggc atgggccttt cccccgccgc cgacatcacg ctctccggcg aggcccacgg
     4501 ggccgagcgg ccggagggtt ccgtggagga cctggtgctg aaggcgctgg aaatccatcc
     4561 cgagttggcc atggccgacc ggcaggtcgt gatccaggac caccgggtac gccaggcgtt
     4621 caccgctttc ctgcccacga tttccctttt ttcttccggc gaatggaccg gcaacgacct
     4681 ggccgcccat gccgaaaact gggtctcggg cttccagggc gcgtggacgc tgttcgacgg
     4741 cctggccaac gtggcgcgat acaaggccgc caaggttgaa cgccggaaaa cccagatcga
     4801 gcgggagaac accttcctct ccatcatggt cggcgtcgtc gccgccgagg ccgccgtgcg
     4861 cgacgccgcc gagggcgccc gcgtccgccg gcgggcctac gacgtggccg ccgc
//
LOCUS       DAOOTK010000180         4898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_10793_length_4898_cov_4.209581, whole genome shotgun sequence.
ACCESSION   DAOOTK010000180 DAOOTK010000000
VERSION     DAOOTK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_4898_cov_4.209581"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK634_13675"
     CDS             complement(<1..595)
                     /locus_tag="PK634_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98825.1"
                     /translation="MKIHRFWLLAAGAALVLGAGWALGGPLHAVQPDDRMAGAWVTEM
                     DSLKDYMTSVLEPINGECTSIEGPIRYEFAPGASHTLTISAFRTVVAITKRKLSGGAD
                     KIVITYTGGFQAPYSVSASELILDIGTRPDFSIFTVDSININGMELPGGEMNLTDVRA
                     PGVAASMRFEFLDDNRLQLTPIFPPAPDGVNIHPRPFI"
     gene            complement(731..4357)
                     /locus_tag="PK634_13680"
     CDS             complement(731..4357)
                     /locus_tag="PK634_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOW98826.1"
                     /translation="MKRWMILAAMGAGLACGARAELNFQEVILDDTYFAYERDVGDVN
                     GDGLNDVIAVQEGQTSLQVFMAPDWTRSVLVSITGTYSYPRADDFKTADLDGDNDLDV
                     ITRLGDSPSSDGAGIAVWYENLGGGTNWAQRPIGNSLEYVKDIVVADFDRDDRPDVAM
                     RMDSRTQLWLQEAGGTWTEVLISHASHEGMEAGDLDNDGDPDLILNGFWFPTPDSPAA
                     CRIAACYTNRTVDAAWFNQTGDWTANSCKVVVGDFDGDGSNDVAFSQSERAGYAVTWY
                     RSPTPLVDGTWTGRAVVVVDYCHNLQATDWDLDGDDDLLVGGMASSTHRGLKLMLNGG
                     AGLAWSEHVIQSDGSYSAETGDIDNDGDPDIVGIYNWNSAPTWIYRNNAGGPPSLDFW
                     CYIEAGANHVRTFGLDAMDADGDGDLDLSSGPYVYRNPGGAMTGAWTRATAFGGGHLF
                     LAANVDGDDRADLLGLQDNAGGNRIDLYWIEAADAGATGWAAPVLFGDVPHSDHAEGF
                     QGYRLAQFVAGGREEIVISTMQGIYYFEIPVSPGTGSWPRVQVCANDSDEGIGVADVD
                     GDTDLDIAFTSGGSKTVLWARNPGDGSGNWPVFTVGSFPEADWPDRCAIADLNGDGRP
                     DIAATEENSGAAADALACWWEQPAAGPTNAGWTRRTIATQYTMNSLDAADFDWDGDTD
                     LSLAEHRGTEKIQVWENDGAGSFAVHEVGSGRESHLGARAFDLDGDGDPDLVSIAYDD
                     YTKLHVWRNDSPSGEPTVARPVIAPNGGAFDEPLSVTLTCATAGAEIWCTTDGSTPTN
                     QPGPSALYTNALSVTTSVTLKARGFKTGHVPSAIASAAFTGPQVLTPTIAPPGGSFLG
                     TQSVTIACATTGVVIRFTLDGSDPDESDAAYGGAIELTNSATVKTRAFRNGLAPSAIA
                     EASFVLLQYGAVAHWRLDERFGTVTMDSSGSGRTGLVSGAAWTAGTKDNALAFDGADD
                     RVNVGAWDVAGTALTICAWVRLDPATVDNDPRLVSKAVGSAEQDHTWMLSLTTVGADR
                     RLRARLKTGGSTTTLIASSGSLALDAWHHAAMTYDGSALRLFLDGAEVGSAAKSGEIT
                     AAPSVEIWLGANPPNAYAPLHGLLDDVRIYNTSLDAAAIQAVMNETPSGPPPEIQSTG
                     GEPAGWTLQVQGEAGHYYILQRTLDLAPPEWINLSTSAATEPAIQIPATGDMPRAYFR
                     VWKH"
     gene            4478..>4898
                     /locus_tag="PK634_13685"
     CDS             4478..>4898
                     /locus_tag="PK634_13685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOW98827.1"
                     /translation="MNTAPHYRAILHAVQEPILVIDRQMRIVEANEAAQKVFSKGSAP
                     LTGCACHRATHGLLQPCSETGLHCPVIEVFETGRFVRAVHRHQRVDGGVVWEEILASP
                     LRNEQGEIVAVVEELRDLTEALQHRSLIKELQAQVRRL"
BASE COUNT          722 a         1671 c         1684 g          821 t
ORIGIN      
        1 tgatgaacgg ccggggatgg atgttcaccc cgtccggcgc cggggggaag atcggcgtca
       61 gttgcagccg gttgtcgtcc aggaactcga agcgcatgct cgccgccacg cccggcgcgc
      121 gcacgtccgt caggttcatc tcgccgccgg gcagttccat gccattgatg ttgatcgaat
      181 ccacggtgaa gatgctgaaa tcgggccgcg tgccgatgtc gaggatcagc tcgctcgcgc
      241 tgacggagta gggggcctgg aagccgcccg tgtaggtgat gacgatcttg tcggccccgc
      301 cggaaagttt gcgcttggtg atggccacca cggtcctgaa ggccgagatc gtcagcgtgt
      361 gcgacgcgcc cggcgcgaac tcgtagcgga tgggcccctc gatgctcgtg cactccccgt
      421 taatgggctc gaggacggag gtcatgtagt ccttcaggct gtccatctcc gtcacccacg
      481 cgcccgccat ccggtcgtcc ggctggacgg cgtgcagggg ccctccgagt gcccagccag
      541 cgcccagaac cagtgccgcg cccgccgcaa ggagccaaaa ccggtggatc ttcatgatat
      601 ttcctttcct gcgtcgttcc ttgaacagga tcacgggaag aagcctacag gaaggaaccg
      661 cgaaatacac gaaagacacg aaaggggcca aggccccgat cctctttagc gtatttcatg
      721 tctttcgtgg ttaatgcttc cacacccgga agtacgcccg gggcatgtcg cccgtcgcgg
      781 gaatctggat cgccggctcc gtcgccgcgc tggtcgagag gttgatccat tccggcggcg
      841 cgagatccag cgtccgctgc aggatgtagt aatgcccggc ctcgccctgc acctggagcg
      901 tccagccggc cggctcgcct cccgtgctct gaatctccgg cggcgggccg gacggggtct
      961 cgttcatcac ggcctggatc gccgccgcgt cgagcgaggt gttgtagatc cggacgtcgt
     1021 ccaggaggcc gtgcaggggc gcgtaggcgt tcggcgggtt ggcgcccagc cagatctcga
     1081 ccgacggcgc cgccgtgatc tccccgctct tcgccgcgct gccgacctcc gcgccgtcca
     1141 ggaacaaccg cagcgcgctc ccgtcgtacg tcatcgccgc gtgatgccac gcgtcgagcg
     1201 ccaggctgcc gctggacgcg atcagcgtcg tcgtgctgcc gccggtcttc agccgcgcgc
     1261 gcagccgccg gtccgcgccg accgtggtca gcgagagcat ccaggtgtga tcctgctccg
     1321 cgctgccgac ggccttggac acgagccgcg ggtcgttgtc gacagtggcc gggtcgagcc
     1381 ggacccacgc gcagatggtc agcgccgtgc ctgcgacgtc ccacgcgccc acgttgacgc
     1441 ggtcgtccgc gccgtcgaag gcgagcgcgt tgtccttcgt gcccgccgtc catgccgcgc
     1501 cggagacgag acccgtgcgc ccgctgccgc tggaatccat cgtgaccgtc ccgaaccgct
     1561 cgtccagccg ccaatgcgcc accgcgccgt attgcaggag gacgaacgac gcctcggcga
     1621 ttgcgctagg ggccagcccg ttgcggaacg cccgcgtctt gacggtcgcc gagttcgtca
     1681 attcgatcgc gccgccgtag gccgcgtccg attcgtccgg gtccgagccg tcgagcgtga
     1741 agcggatcac gacgcccgtc gtcgcgcagg cgatggtcac cgactgcgtg cccaggaacg
     1801 acccgcccgg cggcgcgatc gtcggggtca ggacttgcgg cccggtgaac gccgccgagg
     1861 cgatcgcgct cgggacgtgg cccgtcttga acccgcgggc cttgagcgtc acggaggttg
     1921 tcaccgacag cgcgttggtg tagagcgccg aggggccggg ctggttcgtc ggggtcgagc
     1981 cgtcggtcgt gcaccagatc tccgcgccgg ccgtggcgca ggtgagcgtg acggagagcg
     2041 gctcgtcgaa ggccccgccg ttcggcgcga tgaccggccg ggctacggtc ggctcgccgg
     2101 agggcgagtc gttccgccag acgtgcagct tcgtgtagtc gtcgtacgcg atgctgacga
     2161 ggtccgggtc gccgtcgccg tcgagatcga acgcccgtgc gccgagatgg ctctcgcggc
     2221 cggagccgac ctcgtggacg gcgaacgagc ccgcgccgtc attttcccat acctggatct
     2281 tttccgtgcc gcggtgctcg gcgagggaga ggtccgtgtc gccgtcccag tcgaaatccg
     2341 cggcgtccag gctgttcatc gtgtactgcg ttgcgatcgt ccggcgcgtc cagccggcgt
     2401 tggtcgggcc ggcggcgggc tgctcccacc agcaggccag cgcgtccgcc gccgcgccgg
     2461 aattctcctc cgtggcggcg atgtccggcc ggccgtcgcc gttgaggtcc gcgatcgcgc
     2521 agcggtcggg ccagtcggcc tcggggaacg agccgacggt gaacacgggc cagttcccgg
     2581 agccgtcgcc cgggttccgc gcccacagga ccgtcttgct gccgccgctg gtgaaggcga
     2641 tgtcgaggtc cgtgtcgccg tccacgtccg cgacgccgat cccctcgtcg gaatcgttcg
     2701 cgcagacttg gacgcgcggc caggagcccg tgccgggcga gaccgggatc tcgaaatagt
     2761 aaatcccctg catcgtcgag atcacgattt cctcgcggcc gccggcgacg aactgcgcga
     2821 gccggtagcc ctggaagccc tcggcgtggt cgctgtgcgg gacgtcgccg aaaagcaccg
     2881 gcgcggccca gcccgtcgcg cccgcgtccg ccgcctcgat ccagtagaga tcaatccggt
     2941 tcccgcccgc gttgtcctgc aggccgagca ggtccgcccg gtcatcgccg tccacgttcg
     3001 cggccaggaa gagatgcccg ccgccgaacg ccgtcgcgcg cgtccacgcg ccggtcatcg
     3061 cgccgccggg attccggtag acgtacgggc cgctggaaag gtcgaggtcg ccgtcgccgt
     3121 ccgcatccat ggcgtcgagg ccgaacgtgc gcacgtgatt ggcgccggcc tcgatgtagc
     3181 accagaagtc caggctcggc gggccgccgg cgttgttgcg gtagatccag gtcggggccg
     3241 agttccagtt gtagatgccg acgatgtccg ggtcgccgtc gttgtcgatg tcgcccgtct
     3301 ccgcgctgta gctgccgtcg gactggatga catgttcgct ccaggccagg cctgcgccgc
     3361 cgttcagcat cagtttcagg ccgcggtgcg tggaactggc catgccgccg acaagcaggt
     3421 cgtcgtcgcc gtcgaggtcc caatccgtgg cctgcaggtt atgacaataa tccacgacca
     3481 cgaccgcgcg cccagtccac gtcccgtcca cgagcggggt gggcgagcga taccacgtca
     3541 ccgcgtagcc cgcgcgctcg gactgggaga acgcgacgtc gttcgacccg tcgccgtcga
     3601 aatcgccgac gacgaccttg cacgagttgg ccgtccagtc gccggtctgg ttgaaccagg
     3661 cggcgtccac ggtcctgttc gtgtagcacg ccgcgatgcg gcaggcggcg ggggagtcgg
     3721 gcgtcgggaa ccagaagccg ttcaggatga ggtccgggtc gccgtcgttg tccaggtcgc
     3781 cggcctccat gccctcgtgc gaggcgtggc tgatcagcac ctccgtccac gtgccgccgg
     3841 cctcctgcag ccagagctgg gtccggctgt ccatgcgcat cgcgacgtcc gggcggtcgt
     3901 cccggtcgaa atccgcgacg acgatgtcct tcacatattc gaggctgttg ccgatcggac
     3961 gctgggccca gttcgtaccg ccgccgaggt tctcgtacca gaccgcgatc ccggccccgt
     4021 cggaggacgg gctgtcgccg agccgcgtga tcacgtcgag gtcgttgtcg ccgtcgaggt
     4081 ccgcggtctt gaaatcgtcc gcgcggggat agctgtacgt gccggtgatg gaaacgagga
     4141 cggagcgcgt ccagtccggg gccatgaaca cctggaggct ggtctggccc tcctggacgg
     4201 cgatcacgtc gttgagtccg tcgccgttga cgtcgccgac gtcgcgctcg taggcgaagt
     4261 aggtgtcatc caggatcacc tcctggaaat tcagctccgc ccgggccccg caggccagcc
     4321 ccgcccccat cgccgcgagg atcatccatc gcttcatcac cagcaagata cacagtggcg
     4381 caaaaaaggt agagccggcc ttatgccggc tatcaggagc ccgcctcgat tttttcggtg
     4441 cacgtgcaaa cggctgaaag aggcgtacat tgccttgatg aatacagccc ctcattaccg
     4501 ggccattctg cacgccgtcc aggagcctat tctcgtcatc gaccggcaga tgcggatcgt
     4561 ggaggcgaac gaggcggcgc agaaggtctt ttccaaggga tccgctccgc tgacggggtg
     4621 tgcgtgccac cgggccaccc acgggttgct ccagccctgc tcggaaaccg ggctgcattg
     4681 ccccgtgatc gaggtcttcg agaccgggcg gttcgtgcgc gccgtgcatc ggcaccagcg
     4741 ggtggatggc ggcgtggtct gggaggaaat cctcgcctcg ccgctgcgga acgagcaggg
     4801 cgagatcgtg gccgtggtcg aggaactgcg cgacctgacc gaggcgcttc agcaccggag
     4861 cctgatcaag gaattgcagg cgcaggtcag gcggctcg
//
LOCUS       DAOOTK010000181         4850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_10950_length_4850_cov_4.688425, whole genome shotgun sequence.
ACCESSION   DAOOTK010000181 DAOOTK010000000
VERSION     DAOOTK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_4850_cov_4.688425"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /gene="rpsC"
                     /locus_tag="PK634_13690"
     CDS             <1..510
                     /gene="rpsC"
                     /locus_tag="PK634_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOW98828.1"
                     /translation="VPEVLIERYANRARVTIYTARPGIVIGRKGEAIEQVRAAIADAT
                     KKEIYVEIREVKSPDTNAQLVAENIALQLERRVSFRRALKRAVQMAIELGVLGIKVHV
                     GGRLGGAELARREWYKEGKVPLHTLRANVQYGFAEAHTVTGKIGCKVWICKKEEEETV
                     KEKTHAAYA"
     gene            494..907
                     /gene="rplP"
                     /locus_tag="PK634_13695"
     CDS             494..907
                     /gene="rplP"
                     /locus_tag="PK634_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOW98829.1"
                     /translation="MPLMPKRVKYRKAQRGNRSGFAQSGCTLEFGEYGLKALERAWVT
                     AIQLEACRVAINRELKRKGKMWIRVFPDKPISKKPLEVRMGKGKAAVDKWVAVIKPGR
                     VLFEVDGISEEQAREALRLAATKLPIYTQFIRRGR"
     gene            927..1124
                     /gene="rpmC"
                     /locus_tag="PK634_13700"
     CDS             927..1124
                     /gene="rpmC"
                     /locus_tag="PK634_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOW98830.1"
                     /translation="MKTSELKELTADELRQRLEESQQELFNLRVQQATGQMEKASRLK
                     TLRRDIARVLTLLQQRQKAGR"
     gene            1127..1417
                     /gene="rpsQ"
                     /locus_tag="PK634_13705"
     CDS             1127..1417
                     /gene="rpsQ"
                     /locus_tag="PK634_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOW98831.1"
                     /translation="MASTRQSVRKEREGVVISEAMDKTIVVRVERRYHHPVYGKVIRQ
                     HAKFYAHDEKNEAHKGDVVRIQEIRPISKLKRWRLLEIVRRGSAAAPVESAP"
     gene            1414..1779
                     /gene="rplN"
                     /locus_tag="PK634_13710"
     CDS             1414..1779
                     /gene="rplN"
                     /locus_tag="PK634_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOW98832.1"
                     /translation="MIAMRTNLDVADNTGARRVQCIHVLGQRKRYAHIGDLIKVAVKE
                     AQPVGMVKKGEVHSALIVRTKQGIRRPDGSLLRFDHNAVVLVDEQMNPRGTRIFGPVA
                     RELREKNFMKIISLAPEVI"
     gene            1779..2069
                     /locus_tag="PK634_13715"
     CDS             1779..2069
                     /locus_tag="PK634_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOW98833.1"
                     /translation="MKKSPEKFKMHIKRGDTVLVLSGSDAGKTGKVLQILPKQGRALV
                     EGVRLVTKHLRKSQDNPKGAIVKKEAPIHVSKLKRQAAAAKAPAARPEKKAK"
     gene            2093..2656
                     /gene="rplE"
                     /locus_tag="PK634_13720"
     CDS             2093..2656
                     /gene="rplE"
                     /locus_tag="PK634_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOW98834.1"
                     /translation="MSSLRERYKTEVVKALKESRGYRNPMQVPRLLKIVVNMSVKATE
                     EKDVLKNVAEDLAKITGQKPVITKSRKSISNFKLRAGMPIGAKVTLRGPMMFDFLERM
                     IAAVLPRIRDFRGVPRTFDGRGNFTLGLKEQTLFPEIPADHVKRTQGMDITIVTSAAT
                     DGEAEELLKLLGVPFASAAAGKEGRVG"
     gene            2649..2834
                     /locus_tag="PK634_13725"
     CDS             2649..2834
                     /locus_tag="PK634_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOW98835.1"
                     /translation="MAKKSLIVKANRKPKFAARTIRRCRKCGRPRAYMRKFQLCRICF
                     RELALEGKIPGVVKASW"
     gene            2857..3255
                     /gene="rpsH"
                     /locus_tag="PK634_13730"
     CDS             2857..3255
                     /gene="rpsH"
                     /locus_tag="PK634_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOW98836.1"
                     /translation="MSVCDPISDMLTRIRNAGRAGLATAEMPHSKLKGEIARILKKEG
                     FIADYVSEGQGGKKQLRVYMKYGAGRAPVIRGLRRISKPGLRRYADLKKTPRVVGGMG
                     IAIISTSKGLMTDREMRQNRLGGEVLCYVW"
     gene            3271..3804
                     /gene="rplF"
                     /locus_tag="PK634_13735"
     CDS             3271..3804
                     /gene="rplF"
                     /locus_tag="PK634_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOW98837.1"
                     /translation="MSRIGRQPVVIPQGVTVTVQGRKVLVKGPKGELAWECAPYVSAA
                     VKDGKVEIACERKDQQGSATHGTTRSLIANMVRGVKDGYSKTLEIQGVGFRAALQGKK
                     LVLTLGYSHPIEFPLPDGISAKVEEGNIIVSGADKHLVGDVSARLRSFYPPEPYKGKG
                     VRLKGEYVRRKVGKTVA"
     gene            3806..4171
                     /gene="rplR"
                     /locus_tag="PK634_13740"
     CDS             3806..4171
                     /gene="rplR"
                     /locus_tag="PK634_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOW98838.1"
                     /translation="MKLKTRIDYVVRRHLRVRRKVNGTAERPRLCVHVSNKHMRVQFV
                     DDAQARTLASFSTLTPAVRDAVKGKNNVAAAKELGAYAGKAAREKGIEAVVFDRGGFA
                     YKGRVKALADAARAAGLKF"
     gene            4230..4691
                     /gene="rpsE"
                     /locus_tag="PK634_13745"
     CDS             4230..4691
                     /gene="rpsE"
                     /locus_tag="PK634_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOW98839.1"
                     /translation="MEERVVNVNRCAKVVKGGRRFSFSALVVVGDRKGHVGYALGKAN
                     EVADAIRKATEAARRSMFPVTMKDKTIPHAVVGEMGASRVMLRPASPGTGVIAGGGVR
                     AILELSGVRDVLAKSLGATNALNVIKATVNGLQQLRSREEVAALRQMESVS"
     gene            4694..>4850
                     /locus_tag="PK634_13750"
     CDS             4694..>4850
                     /locus_tag="PK634_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOW98840.1"
                     /translation="MTLSNLTSTPGSRHRRLRVGRGRSSGKGKTSGKGHKGQMSRTGH
                     KRKPTFEG"
BASE COUNT          987 a         1517 c         1699 g          647 t
ORIGIN      
        1 gtcccggaag tgctgatcga gcggtacgcc aaccgcgcgc gcgtgacgat ctacacggcg
       61 cggccgggca tcgtgatcgg gcgcaagggc gaggccatcg agcaggtgcg ggcggcgatc
      121 gcggacgcga cgaagaagga gatctacgtc gagatccgcg aggtcaagag cccggacacc
      181 aacgcgcagc tggtggcgga gaacatcgcg ctgcagctgg agcgccgggt gtcgttccgc
      241 cgcgcgctga agcgggcggt gcagatggcg atcgagctgg gcgtgctggg catcaaggtg
      301 cacgtcggcg gccgcctggg cggggccgag ttggcgcggc gcgagtggta caaggagggc
      361 aaggtccccc tgcacacact gcgcgccaac gtccaatacg ggtttgcgga ggcgcacacg
      421 gtgacgggca agatcggctg caaggtctgg atctgcaaga aggaagaaga agagacggtg
      481 aaggagaaga cccatgccgc ttatgcctaa aagggtgaag taccgcaagg cgcagcgggg
      541 aaatcggtcg ggcttcgcgc agtccgggtg tacgttggag ttcggcgagt acgggttgaa
      601 ggccctggag cgcgcctggg tcacggcgat ccagctcgag gcgtgccgcg tggcgatcaa
      661 ccgcgagctg aagcgcaagg gcaagatgtg gatccgcgtc ttccccgaca agccgatctc
      721 gaagaagccc ctggaagtgc gcatgggcaa gggcaaggcg gccgtggaca agtgggtcgc
      781 cgtgatcaag ccgggccggg tgctgttcga ggtggacggc atctccgagg agcaggcccg
      841 ggaggccctg cggctggcgg ccacgaagct cccgatctac acgcagttta tccggcgcgg
      901 ccggtaacgc cggggatgga atcggcatga agacaagcga actgaaggaa ttgaccgccg
      961 acgaattgcg gcagcggctg gaggaatcgc agcaggagct gttcaacctc cgcgtccagc
     1021 aggcgacggg ccagatggaa aaggcctcgc gcctcaagac cctgcggcgg gatatcgccc
     1081 gggtgctgac cctgctgcag caacggcaga aggcgggcag gtgattatgg caagcacaag
     1141 gcagagcgta cgcaaggaac gcgagggcgt cgtcatcagc gaggccatgg acaagaccat
     1201 cgtcgtccgg gtggagcgtc ggtaccacca ccccgtgtac ggcaaggtca tccggcagca
     1261 cgccaagttc tacgcccacg acgagaagaa cgaagcgcac aagggcgacg tggtccggat
     1321 ccaggagatc cgcccgatca gcaagctgaa gcgctggcgg ctgctggaga tcgtccggcg
     1381 cggctcggcg gcggcgcccg tggagtcggc cccatgatcg cgatgcgaac caacctggac
     1441 gtggcggaca acaccggggc gcggcgggtg cagtgcatcc acgtgctcgg gcagcgcaag
     1501 cggtacgccc acatcggcga cctgatcaag gtcgccgtca aggaggccca gcccgtcggc
     1561 atggtgaaga agggcgaggt gcatagcgcg ctgatcgtcc gcaccaagca gggcatccgg
     1621 cggcccgacg gctcgctgct ccgcttcgac cacaacgccg tggtcctggt ggacgagcag
     1681 atgaacccgc gcggcacgcg catcttcggc cccgtggccc gcgagctgcg cgaaaagaac
     1741 ttcatgaaga tcatttccct ggcacccgag gtcatctagt gaaaaagagc ccggaaaaat
     1801 tcaagatgca catcaagcgc ggcgacacgg tcctggtcct ctccggatcg gacgccggca
     1861 agacgggcaa ggtcctccag atcctcccga agcagggccg cgcgctggtc gagggcgtcc
     1921 gcctggtgac caagcacctg cgcaagagcc aggacaatcc caagggcgcg atcgtcaaga
     1981 aggaagcccc catccacgtg tcgaaactca aacggcaggc ggccgcggcg aaggccccgg
     2041 cggcgcgtcc ggaaaaaaag gcgaagtaac ccacgacgcc aggcggtgaa tcatgtcgag
     2101 cttgcgagaa cgctataaaa cggaagtggt caaggccctc aaggagtcgc ggggataccg
     2161 caaccccatg caggtgccgc ggctgctcaa gatcgtggtc aacatgagcg tgaaggccac
     2221 ggaggagaag gacgtcctca agaacgtcgc ggaggacctc gcgaagatca ccggccagaa
     2281 gccggtcatc acgaagtccc ggaagagcat ctcgaatttc aagctgcgcg ccgggatgcc
     2341 gatcggggcc aaggtgacgc tgcgcgggcc gatgatgttc gatttcctcg agcgcatgat
     2401 cgccgccgtg ctgccccgca tccgtgactt ccgcggcgtg ccgcggacgt tcgacgggcg
     2461 gggcaatttt acgctgggcc tcaaggagca gacgctgttc ccggagatcc cggcggacca
     2521 cgtgaagcgg acccagggca tggacatcac catcgtcacc agcgcggcca cggacggcga
     2581 ggccgaggag ctgcttaagc tcctgggcgt gccgttcgcg agcgcggccg cgggaaagga
     2641 aggacgcgtt ggctaagaaa tcgttaatcg tcaaggcgaa ccggaagccg aagttcgcgg
     2701 cgcgcacgat ccgccgctgc cgcaagtgcg gccggccccg cgcctacatg cggaagtttc
     2761 aactgtgccg tatctgtttc cgggaactcg ccctcgaggg caagatcccc ggcgtcgtga
     2821 aggcgagctg gtaggagccg ggggaaagaa aagaccatga gcgtatgtga tcccatttca
     2881 gatatgttga cgcggatccg caacgccggc cgggccggct tggcgaccgc cgagatgccg
     2941 cactcgaagc tgaagggcga aatcgcccgc atcctgaaaa aggaaggctt catcgccgac
     3001 tacgtcagcg aggggcaggg cggcaagaag cagctccgcg tgtacatgaa gtacggggcc
     3061 ggccgcgccc ccgtcatccg cggcctccgg cgcatcagca agccgggcct gcggcggtac
     3121 gccgacctga agaagacccc ccgggtcgtc ggcgggatgg gcatcgccat catcagcacc
     3181 tccaagggac tgatgacgga ccgcgagatg cggcagaacc gcctcggcgg cgaggtgctg
     3241 tgctacgtct ggtgaaagcg aggatttgac atgtccagaa tcggaagaca accggtggtc
     3301 atcccgcagg gggtgaccgt gaccgtccag ggccggaagg tcctggtgaa aggccccaag
     3361 ggcgaactgg cctgggaatg cgccccctac gtctcggcgg ccgtcaagga cggcaaggtc
     3421 gagatcgcct gcgagcgcaa ggaccagcag gggagcgcga cccacgggac cacgcgcagc
     3481 ctcatcgcca acatggtccg cggcgtgaag gacggctact ccaagacgct cgagatccag
     3541 ggcgtcggtt tccgcgccgc cctgcagggc aagaaactgg tcctgaccct cggctactcc
     3601 cacccgatcg agttccctct ccccgacggc atctccgcca aggtcgagga agggaacatc
     3661 atcgtcagcg gggccgacaa gcatctcgtg ggcgacgtca gcgcgcggct gcgctccttc
     3721 tacccgccgg agccctacaa gggcaagggc gtgcgcctga agggcgagta cgtgcggcgc
     3781 aaagtgggca agacggtggc ataacatgaa attgaaaacc cgaatcgatt acgtggtccg
     3841 gcggcacctg cgcgtgcgcc ggaaggtcaa cggaacggcc gagcgccccc gcttgtgcgt
     3901 gcatgtctcc aacaagcaca tgcgcgtcca gttcgtggac gacgcccagg cgcgcacgct
     3961 cgcgtccttc tcgaccctga cccccgccgt ccgggacgcg gtcaagggca agaacaacgt
     4021 cgccgccgcc aaggagctgg gcgcgtacgc cggcaaggcg gcccgcgaga agggtatcga
     4081 ggccgtggtg ttcgatcgcg gcggattcgc ctacaagggg cgcgtgaagg ccttggccga
     4141 cgccgcgcgc gccgccgggt tgaagttctg aaaaaggagc acagcgcgtg agaaagccag
     4201 ccaggcagga agtcagagag ccctcggaca tggaagagcg ggtggtgaac gtcaaccgct
     4261 gcgccaaggt cgtcaagggc ggccgtcgtt tcagtttcag cgcgctggtc gtggtcggcg
     4321 accgcaaggg ccacgtgggc tacgccctgg gcaaggccaa cgaggtggcg gacgccatcc
     4381 gcaaggccac ggaagccgcc cggcgcagca tgttccccgt gaccatgaag gacaagacca
     4441 ttccccacgc cgtggtcggc gagatgggcg cctcccgcgt gatgctgcgg ccggcctcgc
     4501 cgggcaccgg cgtcatcgcc ggcggcggcg tccgggcgat cctcgaactc tcgggcgtcc
     4561 gcgacgtgct ggccaagtcg ctcggcgcga ccaacgccct gaacgtcatc aaggccaccg
     4621 tcaacggatt gcagcagctg cgctcccgcg aggaagtcgc ggcgctgcgt caaatggaaa
     4681 gcgtatcatg agcctgactc tttcgaacct caccagcacc cccgggtccc ggcaccgccg
     4741 tctccgcgtc ggccgcggcc ggtcctccgg taagggcaag acttcgggca agggccacaa
     4801 gggccagatg tcccgcaccg gccacaagcg caagccgacc ttcgaaggcg
//
LOCUS       DAOOTK010000182         4802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_11111_length_4802_cov_4.091426, whole genome shotgun sequence.
ACCESSION   DAOOTK010000182 DAOOTK010000000
VERSION     DAOOTK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11111_length_4802_cov_4.091426"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK634_13755"
     CDS             complement(<1..993)
                     /locus_tag="PK634_13755"
                     /inference="COORDINATES: protein motif:HMM:NF022773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HOW98841.1"
                     /translation="MKKLLVAVGIMLSVVSLVQAASWPDNLNRNERRRLGTLMQNTWE
                     GLDEMYSEVSGLPYESTLSRSRTTSSTVGLYLASLCAANRLGYIQEDEAVQRIERILS
                     SLDTLPHWNRIYPDVLPANGQDNDPIPGDGTLASYMNLPAGIMVVRATFPRLARRCTD
                     ILATIPWDAFYDRETRRLYRRFDIDKSSARDPTDPATDDGLRLAHFLMIATGKVPPST
                     WPEAREVTETPLRPIYGSLFLDPRGTCFGKADMLAAWRQIEHALNIGSPAWGWDPEAR
                     VPAAEAPTFPLVHPYVSAQALPFFPREVLNNFEALEEMGALLPPARGLRKSSAGY"
     gene            1119..2489
                     /locus_tag="PK634_13760"
     CDS             1119..2489
                     /locus_tag="PK634_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOW98842.1"
                     /translation="MADHVHFVGVAGVGMSALAQALLARGARVTGSDRFLDQGQPPES
                     LAVLRAAGVELAPQDGSGVTAATRAVVVSTAVEPDNRDVAAARSRGVEVVHRAEMLAR
                     LVRGRRLLAVAGTSGKTTVTGLVGWLLERCGRNPTIVNGGAVLGWKGPRSLGNFRAGR
                     DDLWVIEADESDRSFLQFDPDWEIITNLSRDHFELGETTDLFRSFARRAASGVVCGPG
                     VAGLLRAGQADPGQAMLFEESLELVPRGFVREDVTFEVPLPGRHNVENAWLAVQLCRH
                     LGLHGGPLRMALASFPGIERRLERAGEAAGVAVVDDYAHNPAKIAAAWSTMAGRHARV
                     LGVWRPHGFGPLAAMMPELVESLLRVMRPADQLFLLPVFYAGGTARQTVSSEDLASRL
                     QAAGRTAVAMESYDALEHRVLAVAHPGDLVLVMGARDPELPRFARRMVHSLASRPAGG
                     APAGVL"
     gene            2667..3245
                     /locus_tag="PK634_13765"
     CDS             2667..3245
                     /locus_tag="PK634_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOW98843.1"
                     /translation="MEEALQQRRSIRQYAEKPLGLEQAGQLLWAAQGITRPEDGGRTA
                     PSGGQLYPLTVYLAATRVLDLAPGVYRYEPSGHRLVPVMRNAVEELAQAGPNGAFLRK
                     AAAVLVLVGDAQRAEARYGPGYRSYMDLEAGHASQNVYLQAVALGLGTVAANFFDATQ
                     VRRALRLPKGLEPLSIMPVGIPAAAGSGAAPD"
     gene            complement(3193..4215)
                     /locus_tag="PK634_13770"
     CDS             complement(3193..4215)
                     /locus_tag="PK634_13770"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98844.1"
                     /translation="MKIVLLVDLEAVLPGDPQFEGRTKSSRTEMEAHVAAAVRALGHE
                     AAVIPFGPDVLETMHALKGSGAGLVFNLTERFEGDRRKDLNVAALLELLHLPYTGTGP
                     AGLWLCRDKAAGKRILGYHRIRVPRFAEVPPGRRKPAGCLRFPAIVKPMFEDGSDGIS
                     LSSVVRSASELEERVRMIHEGRNQPAICEEYVEGRELYIGVVGNQKRHAFPPWELLFG
                     RAGEGGPPIATARVKWDPAYREKWNIRYQPAELDPATLRRISLFSRRVFELLKLRDYG
                     RVDLRLTPGGEIVFIEANPNPDISRDAELPQAAALEGMEHAALIGHILNLALRRSPRP
                     PESPRA"
     gene            4384..>4802
                     /locus_tag="PK634_13775"
     CDS             4384..>4802
                     /locus_tag="PK634_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOW98845.1"
                     /translation="MNRAKFYVTTPIYYVNDQPHIGHAFTTILADVLAQYHRLLKVPT
                     HFLTGTDEHGQKVQKAAQAAGITPQEQADRTVVRFQALWKKLHITNDDFIRTTEERHK
                     KVVQKILQDLYDRGEIYRAEYDGWYCVPDERFFMEKDL"
BASE COUNT          718 a         1601 c         1700 g          783 t
ORIGIN      
        1 gtacccggcc gacgacttcc ggagcccgcg ggccggcggg agcagggccc ccatttcctc
       61 cagcgcttcg aagttgttca atacctcgcg cgggaagaag ggcagggcct gcgccgagac
      121 atacgggtgg accagcggga aggtgggcgc ctcggcggcc gggacccgtg cctccgggtc
      181 ccatccccag gccggcgagc cgatgttcag cgcgtgctcg atctggcgcc atgcggccag
      241 catgtccgcc ttcccgaagc aggtgccgcg ggggtcgagg aagaggctgc cgtagatcgg
      301 gcggagcggg gtctcggtca cctctctggc ttccggccag gtcgagggag gaaccttccc
      361 cgtcgcgatc atcaggaaat gcgccagccg cagcccgtcg tccgtggccg ggtccgtcgg
      421 atcacgggcc gagctcttgt cgatgtcgaa ccggcggtac agacgccgcg tctcccggtc
      481 gtagaaggcg tcccagggaa tggtggccag gatatccgtg cagcggcggg ccagccgggg
      541 gaaggtggcg cgcaccacca tgatgcccgc cggcaggttc atgtacgaag ccagggtgcc
      601 gtcgccgggg atcggatcgt tgtcctggcc gttggccggg aggacgtcgg gataaatgcg
      661 gttccagtgc gggagcgtat ccaggctgga caggatgcgc tcgatgcgtt ggacggcctc
      721 gtcctcctgg atgtacccga gtcggttggc cgcgcaaagg gaggccaggt agaggccgac
      781 cgtggacgag gtggtccggc tgcgggatag cgtcgattcg tacgggaggc cgctgacttc
      841 cgagtacatc tcgtcgaggc cttcccacgt gttctgcatc agcgtgccaa ggcggcggcg
      901 ctcgttccgg ttgagattgt cgggccagga cgcggcttgg accagcgaaa cgacggacaa
      961 catgatgccc acggcgacca gtaacttctt cacggcacaa acctcccgga caaaaagcga
     1021 gaccgctcat aggataatgg gccgggggcc caggtcaatc ccgcattcgg ccgctttacg
     1081 gggttgcgcg cggtcaggcc ttgggtcagg atgccggcat ggcggaccat gtccatttcg
     1141 tgggcgtcgc cggcgtcggc atgagcgccc tggcccaggc gctgctggcc cgcggcgcgc
     1201 gcgtcacggg gtcggatcgt ttcctcgacc agggccagcc cccggaatcg ctcgccgtgc
     1261 tccgggcggc gggcgtggaa ctcgcgcccc aggacggctc cggcgtgacg gccgcgaccc
     1321 gggccgtggt cgtcagcacc gccgtggagc cggataaccg cgatgtggcc gccgcccgct
     1381 cgcgcggcgt cgaggtcgtg caccgggcgg agatgctggc gcgcctcgtc cgcggccggc
     1441 ggctgctggc ggtggccggc acgtcgggca agaccaccgt cacggggctc gtcggctggt
     1501 tgctggagcg gtgcggccgg aatcccacca tcgtcaacgg cggcgccgtg ctgggctgga
     1561 aaggcccgcg ctcgctcggc aatttccgcg cggggcgcga cgacctctgg gtcatcgagg
     1621 cggacgaaag cgaccgatcc ttcctgcagt tcgatcccga ctgggaaatc atcactaacc
     1681 tgtcgcgcga tcatttcgag ctgggggaaa cgacggacct tttccgctcc ttcgcgcgcc
     1741 gggccgcgtc gggcgtcgtg tgcgggcccg gcgtggccgg gctcctccgg gccggccagg
     1801 ccgatcccgg ccaggccatg ctgttcgagg aatcgttgga gctggtgccg cgcggattcg
     1861 tccgcgagga cgtgaccttc gaggtgccgc tcccgggccg gcacaatgtc gaaaacgcct
     1921 ggctggccgt tcagttgtgc cggcatctcg gcctgcatgg gggccccctg cgcatggcgc
     1981 tggcttcctt cccgggcatc gagcggcggc tggagcgggc gggggaggcc gcaggagtcg
     2041 ccgtggtgga cgactacgcc cacaacccgg ccaagattgc ggccgcgtgg agcacgatgg
     2101 ccgggcggca cgcccgcgtg ctgggcgtat ggcggccgca cggattcggt cccctcgcgg
     2161 ccatgatgcc cgaactcgtg gaatcgttgc tccgtgtcat gcgtccggcc gatcagcttt
     2221 tcctgctgcc ggttttttac gcgggcggca ccgcgcggca aacggtctcc tccgaggacc
     2281 tggcgagccg cctgcaggcc gccggaagga ccgcggtcgc gatggaatcc tacgatgccc
     2341 tggagcatag agtgctggcc gtcgcgcatc cgggcgatct cgtgctcgtc atgggggccc
     2401 gcgatcccga gttgccgcgc tttgcgcgga ggatggtcca ttccctggcg agccggccgg
     2461 ccggcggggc gccggccggt gtcctttaaa cttcattccg gtgtgctatg ttgctgccgg
     2521 gaaccatgaa gtctcgttcg acatatgggg cgctgtggat ggtgttcgcc ctggctggga
     2581 atccggggcc ggccgcatcc cagccgacgc ccgttccgcc tggggtggtg gagctcccgc
     2641 cgccccgaac gtccggtcgt ctttccgtgg aggaggctct ccagcaacgg cgctccattc
     2701 ggcaatacgc ggaaaaacct ctcggcctgg agcaggctgg acagttgctg tgggccgccc
     2761 aaggcattac ccgccccgaa gacggcggcc gcaccgcgcc ctccggcggc cagttgtatc
     2821 ccctcaccgt gtacctggcc gcgacccgcg tgttggacct ggcgccggga gtctaccggt
     2881 acgagccgtc cgggcaccgc ctggtccccg tcatgcgaaa cgccgtcgag gagctcgccc
     2941 aggccggacc gaacggcgcg ttcctccgta aggccgccgc cgtcctggtc ctggtgggcg
     3001 atgcccagcg ggccgaggcg cgctacgggc cggggtatcg ttcttacatg gatctcgagg
     3061 ccggccacgc gtcccagaac gtgtatctcc aggccgtcgc gctcgggctg ggtaccgtag
     3121 ccgcgaactt cttcgacgcg acgcaggtcc ggcgggcctt gcgtctgccg aaaggcctcg
     3181 agcccttgag catcatgccc gtggggattc cggcggccgc ggggagcggc gcagcgccag
     3241 attgaggatg tgcccgatca gcgccgcgtg ctccatgccc tccagggcgg cggcctgcgg
     3301 gagttcggcg tcgcgcgaga tgtcgggatt cgggttggcc tcgatgaaca cgatctcgcc
     3361 ccccggcgtc agccgcaggt cgacgcgccc gtaatcgcgc agcttgagca gctcgaacac
     3421 gcgccggctg aagaggctta tcctccggag cgtggcgggg tccagttcgg ccggctggta
     3481 gcggatgttc cacttttccc ggtaggcggg gtcccacttg accctcgccg tggcgatcgg
     3541 cggcccgcct tcgccggccc ggccaaagag cagctcccac gggggaaacg catgccgctt
     3601 ctggttgccc accaccccga tgtacagttc ccggccctcg acgtattcct cgcagatcgc
     3661 cggctggttc cgcccctcgt ggatcatgcg gacgcgttcc tcgagttcgc ttgccgagcg
     3721 caccacggag gagagcgaga tgccgtccga accgtcctcg aacatgggtt tgacgatggc
     3781 ggggaaccgc aggcagccgg ccggtttcct ccggccgggg ggcacctcgg cgaaacgcgg
     3841 cacgcggatg cggtggtacc cgaggatgcg cttgccggcg gccttgtccc ggcagagcca
     3901 gagcccggcg gggccggtgc ccgtgtaggg aaggtgcagc agttcgagca gggccgcgac
     3961 gttcagatcc ttgcggcggt cgccctcgaa acgctcggtg agattgaaga ccaagcccgc
     4021 gccggagcct ttcagcgcgt gcatcgtctc caggacgtcg ggcccgaacg ggatcaccgc
     4081 ggcctcatgg cccagcgcgc gcacggccgc cgcgacgtgg gcttccatct cggtgcgcga
     4141 ggacttcgtc cgcccctcga attgcgggtc gccgggcagc acggcctcga ggtccaccag
     4201 cagcacgatc ttcatgcgcg cctttcacgg ccattcaatt ctatttgcgc cccgtgcgca
     4261 agggcttaaa gcggtccgtg ttctgcattt gaacatcggg cggcggggac tataatgccc
     4321 gcgttttgcc aggcccggcc ggcgcaggac gccgggcgga gcagcggacc ggaaaggccc
     4381 gatatgaaca gagcgaaatt ttacgtcacg acaccgattt attatgtgaa tgaccagccg
     4441 cacatcggcc atgccttcac cacgatcctg gccgacgtgc tggcgcagta ccaccgcctg
     4501 ctgaaggttc cgactcattt cctcacggga acggacgagc acggccagaa ggtgcagaag
     4561 gccgcccagg ccgccggtat caccccgcag gagcaggccg accgtacggt cgtgcggttc
     4621 caggccctgt ggaagaagct gcacatcacg aacgacgact tcatccgcac cacggaggag
     4681 cgccacaaga aagtcgtgca gaaaatcctg caggacctgt acgaccgcgg cgagatctac
     4741 cgcgccgagt acgacggctg gtactgcgtg ccggacgagc ggttcttcat ggagaaggac
     4801 ct
//
LOCUS       DAOOTK010000183         4747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_11296_length_4747_cov_4.142583, whole genome shotgun sequence.
ACCESSION   DAOOTK010000183 DAOOTK010000000
VERSION     DAOOTK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11296_length_4747_cov_4.142583"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1805)
                     /locus_tag="PK634_13780"
     CDS             complement(36..1805)
                     /locus_tag="PK634_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOW98846.1"
                     /translation="MTADPSTGAMQKQKEWATFLRLFRYARPYVLRLLLGAVCGVLFA
                     GSMAGMLVALKEVFGRIFNPEEVSWARTLLVVGLLPLLAAVRGIGQFASTYLIEWVGN
                     RVVMDLRVQVFNHLQDLSLLFFTRARTGELISRTTNDTAMIERAVSTVLADLIREPFT
                     LVAMVGFAFWLDWKLALIGVVLFPVCIIPVTLFGRRVRRFSREGQQQLADMVSVLQEN
                     AGGARVVRAFGLEELERARFLKTARGVLNRLMRVVRARAAVDPIIVFISMLGLGLVLI
                     YARWTRMSVQDLFTFAAALVALYDPAKKISRIHISIQQSAGAADRIFELLDTPVSVRP
                     SPGAVLFAGPVQSIVFGDVAFAYDTEPILRAIRMEVKAGQRIAIVGGSGSGKTTLVGL
                     IPRFYDVTAGRILLNGRDIRDFTLDSLRRQIGMVTQDTFLFNETVARNIAFGKPDATR
                     QEIEQAARRAHAHEFIAELPQGYETMVGERGVRLSGGQCQRLAIARAIVRNPPILILD
                     EATSALDTESERQVQAAIEEIMTERTVFAIAHRLSTIMHADRIVVLDGGLIAEVGTHG
                     ELLEKGGLYKRLYDMQFQDTPAA"
     gene            complement(1802..2725)
                     /gene="trxB"
                     /locus_tag="PK634_13785"
     CDS             complement(1802..2725)
                     /gene="trxB"
                     /locus_tag="PK634_13785"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOW98847.1"
                     /translation="MEKVIILGSGAAGLAAAIYTARADLNPLVIEGLQPGGQLTTTTD
                     VENYPGFPEGIDGTMLMENMKAQAKRFGARVVSGTVSAVDFRKKPFRVTLDDGQGFEA
                     RTVIVATGASARYLGLESERKLMGRGVSGCATCDGALYRKVPVAVVGGGDTAMEEAAF
                     LTRFASRVYLIHRRDQFRASKIMADRVLANPKIEVVWNAVVTEVLDVQANEVMGVRVK
                     DVKTGAERVLEVKAMFSAIGHRPNTEAFKGQLAMDEQGYLATRNTRTNVEGVFAAGDV
                     QDPHYRQAVTAAGSGCMAALEAQRYLEAKGE"
     gene            2962..3348
                     /locus_tag="PK634_13790"
     CDS             2962..3348
                     /locus_tag="PK634_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HOW98848.1"
                     /translation="MSHQDWLEEMFERQAAFNQRIGLDCSKLDDAGRQQWLLNYCRAM
                     TQELAELTDCVPWKWWAKYQKFDLQNARVEVVDLFHFLISAAEVLGLTARDVHNLYMQ
                     KNKVNFQRQEKGYTEKNEEDNKAVKL"
     gene            complement(3338..4039)
                     /locus_tag="PK634_13795"
     CDS             complement(3338..4039)
                     /locus_tag="PK634_13795"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOW98849.1"
                     /translation="MRGLHIGRLAVGRVPRIVGTAVSAESLDSAARIAKPAFDLLEIR
                     VDCMDSRWPAGAIRLREAGIPLILTVRSALEGGAWRGAEAERKRVYLSNLAVADAVDV
                     EIRSRALKTVARAARRRGRLVIGSFHDFDGTPSMAVLRRIVWRGRAAGAHVVKIATHV
                     GGEADLERLLDLLAESREPLCVLGMGPRGAAARVRLARAGSCLTYGYVDRPAAPGQPS
                     CRELAGKLRRARLTA"
     gene            complement(4045..4311)
                     /locus_tag="PK634_13800"
     CDS             complement(4045..4311)
                     /locus_tag="PK634_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98850.1"
                     /translation="MAIISTKPVKKCRGCALNLGKRCAVFEHPVLKWKKKCEGFNNPV
                     LIARYEKVQHPEGARARKVERKQRAALAHTVVHSDGVHPLGRAR"
     gene            complement(4397..>4747)
                     /locus_tag="PK634_13805"
     CDS             complement(4397..>4747)
                     /locus_tag="PK634_13805"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98851.1"
                     /translation="ADPLVFTVANLTQYGVGAKIAPRAEPDDGWLELVVVSRQDAPRV
                     LAGLPRLFDGSFDGLPGVETRRFKRMLVERARPAPIQMDGELIEPADKVDIEVVHKAL
                     RVLVPGGSSDGGAD"
BASE COUNT          782 a         1680 c         1538 g          747 t
ORIGIN      
        1 gccggttccg aacgccacgg agtagccggc ctcgttcagg cggccggggt atcctgaaac
       61 tgcatgtcgt acaggcgttt atagagtccg cccttctcca gcagttcccc gtgcgtgccc
      121 acctcggcga tgagaccgcc gtccagcacg acgatgcggt cggcgtgcat gatcgtggaa
      181 aggcggtgcg cgatggcgaa aaccgtccgc tcggtcatga tctcctcgat ggcggcctgg
      241 acctgccgct cggattccgt gtccagcgcg ctggtcgcct cgtcgaggat caggatcggc
      301 ggattgcgga cgatcgcccg cgcgatggcc aggcgctggc actgtccgcc ggacagtctc
      361 acgccccgct cgccgaccat cgtttcgtat ccttgcggca attccgcaat gaactcgtgg
      421 gcatgagcgc gccgggccgc ctgctcgatc tcctgccggg tcgcgtcggg cttcccgaag
      481 gcgatgttcc gggccacggt ctcgttgaag aggaacgtgt cctgggtgac catgccgatc
      541 tgccggcgca agctgtccag cgtgaagtcg cggatatccc ggccgttcag gaggatccgt
      601 ccggcggtca cgtcgtagaa cctcgggatc agccccacca gcgtcgtctt gcccgagccg
      661 gagccgccga cgatggcgat gcgctggccc gctttcactt ccatccggat cgcgcgcagg
      721 atcggttccg tgtcgtaggc aaacgccaca tcgccgaaga cgatcgactg caccggcccc
      781 gcgaacagga ccgcgccggg cgacggccgt acgctgaccg gcgtatccaa caactcgaag
      841 atccggtcgg ccgcgccggc gctctgctgg atgctgatgt gaatgcggct gattttcttg
      901 gcgggatcgt acaacgcgac gagcgcggcg gcgaaggtga acaggtcctg cacgctcatc
      961 cgcgtccagc gggcgtagat gagcacgagg cccagcccca gcatggagat gaagacgatg
     1021 atcgggtcca cggccgcccg ggcgcgaacg acgcgcatca gccggttcag cacgccgcgc
     1081 gcggttttca ggaaccgcgc ccgttcgagt tcctccaggc cgaaggctcg gaccacccgc
     1141 gcgccgcccg cgttttcctg gagcacggag accatgtcgg ccagctgctg ctgcccttcg
     1201 cgggagaacc gccgcacgcg ccggccgaag agcgtgaccg ggatgatgca gaccgggaac
     1261 aacacgacgc cgatcagggc cagtttccag tcgagccaga acgcgaaccc caccatcgcc
     1321 acgagcgtga acggctcgcg gatcaggtcc gccagcacgg tcgagaccgc gcgctcgatc
     1381 atcgcggtgt cgttcgtcgt gcgcgagatc agttcgccgg tgcgcgcccg ggtgaagaac
     1441 agcagcgaca ggtcctgcag gtggttgaaa acctgcaccc gcaggtccat caccacccgg
     1501 ttgccgaccc attcgatgag gtacgtgctg gcgaactggc cgatcccgcg gacggcggcg
     1561 aggagcggga gcaatcccac gaccaggagg gtccgcgccc aggagacctc ctccgggttg
     1621 aagatgcggc cgaagacctc cttcagcgcg acgagcatgc cggccatcga gcccgcgaac
     1681 aggacgccgc agaccgcgcc gagcagcagc cgcagaacat acgggcgggc gtaccggaac
     1741 agccggagga aggtggccca ttccttctgc ttctgcatcg cgccggtgga gggatccgcc
     1801 gtcattcgcc tttcgcttcc aggtagcgct gcgcttccag cgccgccatg catccgctgc
     1861 ccgcggccgt gacggcctgc cggtagtggg gatcctgcac gtcgccggcg gcgaacacgc
     1921 cctcgacgtt cgtccgcgtg ttccgggtcg ccaggtagcc ctgctcgtcc atggcgagct
     1981 ggcccttgaa ggcctccgtg ttgggccggt gaccgatcgc ggaaaacatg gccttgacct
     2041 cgaggacccg ctccgcgccg gtcttgacgt ccttcacgcg cacccccatc acctcgttgg
     2101 cctgcacgtc caggacctcg gtcacgacgg cattccagac cacctcgatc ttgggattgg
     2161 ccaggacgcg gtcggccatg atcttcgagg cccggaactg gtcgcggcgg tggatcaggt
     2221 acacgcggga ggcgaagcgg gtcaggaacg ccgcttcctc catcgccgtg tcaccgccgc
     2281 ccacgacggc gacggggacc ttgcggtaga gcgccccgtc gcaggtcgcg cagcccgaga
     2341 cgccgcggcc catgagcttc cgctcggact ccagcccgag gtagcgggcg ctcgcgccgg
     2401 tggcgacgat gacggtgcgg gcctcgaagc cctggccgtc gtccagcgtc acgcggaagg
     2461 gcttcttcct gaaatccacg gcggataccg tgccggaaac cacgcgggcg ccgaaccgct
     2521 tggcctgggc cttcatgttc tccatgagca tcgtgccgtc gatgccctcg gggaacccgg
     2581 gatagttctc gacgtcggtg gtcgtcgtga gctgtccgcc gggttgaagc ccctcgatga
     2641 ccagcggatt caggtcggcc cgcgccgtgt agatcgcggc cgccaggccc gccgccccgc
     2701 tgcccaggat gatcactttt tccatgaccc gctctcctcg cgtccgaagg ttcgccgtct
     2761 tgatagcaca acgcggcgcg aaaggcagca gaaatggcgc ccccccctcc catcccggcc
     2821 gctgcagcgt aacatttcga ttcacgggtg tgaatgatta tgttacgccc cgccctcctc
     2881 cgttccggcg cgcgccgggc gcagtcggtg cttgcgcggc gcgcgggctt cggcttcact
     2941 gctgccgaca ggagaaaccc catgtcccat caagactggc tggaagagat gttcgagcgg
     3001 caggcggcgt tcaaccagcg catcggcctg gactgctcga agctggacga cgcgggccgc
     3061 cagcagtggc tgctgaacta ctgccgggcc atgacgcagg aactggccga actcaccgac
     3121 tgcgtcccct ggaaatggtg ggcgaagtac cagaagttcg acctccagaa cgcccgcgtc
     3181 gaggtcgtgg acctgttcca cttcctgatc tccgcggcgg aggtccttgg cctgaccgcc
     3241 cgcgacgtgc acaacctgta catgcagaag aacaaggtca acttccagcg ccaggagaag
     3301 ggctacacgg agaagaacga ggaggacaac aaggccgtca agctgtgagg cgcgcgcgcc
     3361 gcagtttccc ggccagttcc cggcacgacg gttgtccggg cgccgcgggg cggtcgacgt
     3421 agccgtaggt caggcacgac cccgcgcggg ccagccgcac ccgcgccgcc gcgccgcgcg
     3481 ggcccatgcc gagcacgcac aacggctccc tcgattccgc cagcagatcg agcaaccgtt
     3541 cgaggtcggc ttcgccgccg acgtgcgtgg ctatcttcac gacgtgggcg ccggccgccc
     3601 gcccccgcca cacgatccgc cgcaacacgg ccatggaagg cgtgccgtcg aaatcgtgga
     3661 acgacccgat caccagccgc ccccgacgcc gcgccgccct cgcgacggtc ttcagcgccc
     3721 ggctgcgaat ctccacgtcc accgcgtccg ccacggccag gttcgagagg tagacccgct
     3781 tgcgttccgc ctccgcgccg cgccaggccc cgccctcgag cgcggagcgc acggtgagga
     3841 tcaagggaat gcccgcttcc cgcagccgga ttgcgccggc tggccacctg gaatccatgc
     3901 agtccacgcg aatctccagg agatcgaacg ccggtttcgc gatccgggcg gcggaatcga
     3961 gcgattccgc cgagacggcc gtccccacga tgcgcgggac acggccgacg gccagccggc
     4021 cgatgtggag gcccctcatg cgggttaccg tgcgcggccc agcggatgaa ccccgtcgct
     4081 gtgaaccacc gtgtgcgcca gcgcggcgcg ctgcttgcgc tccaccttcc gcgcgcgcgc
     4141 cccttccgga tgctgcacct tctcgtagcg ggcgatcagg accgggttgt tgaacccctc
     4201 gcatttcttt ttccacttca ggacgggatg ttcaaaaacc gcgcatcgct tgccgaggtt
     4261 caacgcacac ccgcgacatt tcttcaccgg cttggtgctg atgatggcca cgtggaaccc
     4321 tcctttctgc gaatcgctct cattctgctc cttatgttta gtataccacc ggggaccggg
     4381 ccggtacagg gggaaatcaa tcggccccgc cgtccgaaga cccgccgggg accagcacgc
     4441 ggagcgcctt gtgcacgacc tcgatgtcga ccttatcggc cggctcgatg agttcgccgt
     4501 ccatctggat cggcgccggc cgggcgcgct ccacaagcat ccgcttaaac cggcgggtct
     4561 ccacgcccgg caggccgtcg aaggaaccgt cgaacagccg cggcaacccg gccagcacgc
     4621 gcggcgcgtc ctggcgcgac acgaccacga gttccagcca cccgtcgtcc ggttcggcgc
     4681 gcggagcgat cttggccccc accccgtatt gcgtcaggtt ggccacggta aagaccagag
     4741 gatcggc
//
LOCUS       DAOOTK010000184         4747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_11297_length_4747_cov_3.927110, whole genome shotgun sequence.
ACCESSION   DAOOTK010000184 DAOOTK010000000
VERSION     DAOOTK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_4747_cov_3.927110"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /gene="mtnA"
                     /locus_tag="PK634_13810"
     CDS             <1..882
                     /gene="mtnA"
                     /locus_tag="PK634_13810"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007127359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOW98852.1"
                     /translation="SDTPGLLKAVVKGASYLAGSRPTAVNLFNMLERMKKVAAGLAGL
                     PPDRFREALAKEAQAIRDEDAAMCRAIGRHGLTLLRDGFTVLTHCNAGGLATAEYGTA
                     LAPVYLARERGWKVRVFADETRPLLQGARLTAWELMQAGVDVTLICDNMAASVMAAGK
                     IDAVLVGADRIAANGDTANKIGTYSLAVLAAAHEIPLYVLAPTTTFDPAIPDGRHIPI
                     EHRPAEEVTEGMGRRTAPRGVKVHSPAFDVTPASLVRAIVCERGIARPPYTKSLAGLL
                     KSGASAGPVARKRRAAS"
     gene            879..1814
                     /gene="thiL"
                     /locus_tag="PK634_13815"
     CDS             879..1814
                     /gene="thiL"
                     /locus_tag="PK634_13815"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOW98853.1"
                     /translation="MTTFRQLGEREVIRRLARLVPDRADVRVGIGDDVAVVGHAGAYD
                     LLLTSDAVIENTHFLPDAPPESIGHKAIGRVLSDFAAMGGEPLWALVDLVAPAETPVE
                     WAESVYRGAARLAARFHLAIVGGDTTGGPVRELHVFGVGRLPAGEAVLRSGARPGDVL
                     YVTGALGGSLAGRHLDFEPRVAEGAWLREGRWATAMMDISDGLLVDLERMLERSRAGA
                     ELQSQRVPVAPDALAAKDGRSPLEHALGDGEDYELLFAVPAARVADFEAAWKARFKLP
                     CSSVGTITAEPGRLVLRDRTGRVLTPGRAGYEHFA"
     gene            complement(1815..2012)
                     /locus_tag="PK634_13820"
     CDS             complement(1815..2012)
                     /locus_tag="PK634_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98854.1"
                     /translation="MSGPEITERDRRMAQQCVDCPVCRHARRKQRGLAFWFVKTIEGG
                     LCPYCRAYEKVYGRKAHEPAS"
     gene            complement(2009..2443)
                     /locus_tag="PK634_13825"
     CDS             complement(2009..2443)
                     /locus_tag="PK634_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98855.1"
                     /translation="MDHFTAPPQDPAGNPAPDPDENWPRSGPNIALLLLAILVIAAVV
                     VYAVFRWKNRPEDHSPAAPEVAVGDSRERVVDILGKPRGTTLVGGQEILIYSNGQVII
                     ENGVAASVEIGSGRQRGEVTSEGSQIILQKGGRIVEEEKAGP"
     gene            2572..3642
                     /locus_tag="PK634_13830"
     CDS             2572..3642
                     /locus_tag="PK634_13830"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOW98856.1"
                     /translation="MALSLYLIGAPRSGKTTVFEALTNTPEGRHFSAKGAHHLGMVKV
                     PDARLAALRDMYQPEKYTPAEVSFVDVALPPVPEGGHALSSLAALLGEADAFVLVVQA
                     FGDMDGRGKPLDPAAQLESTALEIVFADLEKVETRLGRIEHEKKRGLKTNPAEEAALQ
                     KGKAALEAGTPLRRVGWNAEEEKTLRTFQFLSMKPALVIANTGEGDLKGETLGALRQA
                     ADRLGCETLAFCAALEAEMAQLEASAQAAFLKDYGLEEPARIRLIQAAYRLLNLISFF
                     TVGEDEVRAWTIRRGTHAQAAAGKVHSDIERGFIRAETVASAELLKTKSWAACRDNAT
                     LRLEGKTYEVQDGDVIHFRFNA"
     gene            complement(3653..3928)
                     /locus_tag="PK634_13835"
     CDS             complement(3653..3928)
                     /locus_tag="PK634_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOW98857.1"
                     /translation="MVTALVLLNVDRDKVNEVAEQLAGQAGISEVYSVAGQYDLVAII
                     RVKDNEGMADLVTKHLLKVRGITRSQTLIAFQVYSKHDLETMFSIGT"
     gene            4012..4254
                     /locus_tag="PK634_13840"
     CDS             4012..4254
                     /locus_tag="PK634_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98858.1"
                     /translation="MRSLRIKTYAAGSEKPKSAITIPLVVARLAGKLIPRAVAEDLAR
                     KGINVVEILDIIRKEGLEGTLMEIEKGGERTVVSVE"
     gene            complement(4280..>4747)
                     /locus_tag="PK634_13845"
     CDS             complement(4280..>4747)
                     /locus_tag="PK634_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017430473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOW98859.1"
                     /translation="DPGLHRTLAGDGLATSLAALLGGPPNTTYSEVTGAVALTRAFEP
                     KYMRIAAVTAMLVAFVSKLGGFLRTIPSPVMGGIMVLLFGMIAAIGAGTLVKAQLDMS
                     KPRNLVIASVILVVGIGDLQVSCGRFAMGGIGLAGIVGILLNLVLPQDGAEKK"
BASE COUNT          730 a         1651 c         1649 g          717 t
ORIGIN      
        1 tccgacacgc cgggcctgct caaggccgtc gtgaagggcg cctcgtacct cgccggttcg
       61 cgccccaccg cggtgaacct gttcaacatg ctcgagcgca tgaagaaggt cgccgccggc
      121 ctggccggcc tgcctcccga ccgcttccgc gaagccctcg cgaaggaggc ccaggcgatc
      181 cgcgacgagg acgcggccat gtgccgggcc atcgggcggc acggcctgac gctgctgcgc
      241 gacggcttca ccgtgctcac gcactgcaac gcgggcggcc tggccaccgc cgagtacggg
      301 accgccctcg cgcccgtcta cctggcccgg gagcgcgggt ggaaggtcag ggtgttcgcc
      361 gacgaaaccc ggccgctgct ccagggcgcg cgcctgaccg cctgggagct gatgcaggcg
      421 ggcgtggacg tgaccctgat ctgcgacaac atggcggcct cggtcatggc cgccgggaag
      481 atcgacgccg tgctcgtcgg cgccgaccgg atcgccgcca acggcgacac ggccaacaag
      541 atcgggacgt actccctcgc cgtgctggcc gccgcccacg aaatcccgct gtacgtcctg
      601 gcgccgacga ccacgttcga tcccgcgatt cccgacggcc ggcacatccc gatcgagcac
      661 cgccccgccg aggaggtcac cgagggcatg ggtcgtcgca cggccccccg cggcgtcaag
      721 gtgcattcgc cggccttcga cgtcacgccc gcgagcctgg tccgcgccat cgtctgcgag
      781 cggggcatcg cgcgcccgcc gtacacgaag agcctggccg gactcctgaa atccggggcc
      841 tcggcgggac ccgtagcccg gaagcgccgc gccgcctcat gaccacgttc cgccagcttg
      901 gcgagcggga agtgatccgg cgcctggccc ggttggttcc ggatcgcgcc gatgtgcggg
      961 tgggcatcgg cgacgacgtg gccgtggtcg ggcacgccgg ggcgtacgac ctgctgctga
     1021 cctccgatgc cgtgatcgaa aacacccact ttttgcccga cgcgccgccg gagtcgatcg
     1081 gccacaaggc catcggcagg gtcttgagcg atttcgcggc gatgggcggc gagccgctct
     1141 gggcgctggt cgatctcgtc gcccccgcgg agaccccggt cgaatgggcc gagtcggtct
     1201 accgcggcgc ggcccgcctg gccgcgcgct tccacctggc catcgtcggc ggcgacacca
     1261 ccggcggccc cgtccgcgag ctgcacgtgt tcggggtcgg ccggctgccg gcgggcgaag
     1321 ccgtgttgcg atccggcgcc aggccgggcg acgtgctgta cgtgacgggc gccctcggcg
     1381 ggagcctggc cggccggcac ctggacttcg agccgcgcgt ggcggagggc gcctggctcc
     1441 gggagggccg atgggccacg gcgatgatgg atatcagcga cgggctgctc gtggacctgg
     1501 agcggatgct ggagcgaagc cgggcgggcg ccgaactcca gtcgcagcgc gtgcccgtcg
     1561 cgccggatgc cctcgccgcg aaagacgggc gcagcccgct ggagcatgcg ctgggagacg
     1621 gcgaggatta cgaactgctt tttgccgtgc cggccgcccg tgttgcggat ttcgaggccg
     1681 cctggaaagc ccgtttcaag ttgccctgtt cctccgtggg gaccatcacc gcggagccgg
     1741 ggcgcctggt gctgcgggat cggaccggcc gcgtgctcac gccgggtcgc gccgggtacg
     1801 agcacttcgc ctgatcagga cgccggctcg tgcgccttgc ggccgtagac cttctcgtag
     1861 gcccggcagt acgggcacag gccgccctcg atggtcttga cgaaccagaa ggccaggccg
     1921 cgctgcttcc ggcgcgcatg ccggcacacg gggcagtcca cgcactgctg cgccatgcgc
     1981 cggtcgcgtt ccgtgatctc cggtccgctc atgggccggc cttctcctcc tcgacgatcc
     2041 gcccgccttt ctgcaggatg atctgcgagc cctcgctggt cacctcgccg cgctggcggc
     2101 cggaaccgat ctcgacgctg gcggcgacgc cgttctcgat gatgacctgc ccgttggagt
     2161 agatcaggat ctcctggccg ccgacgagcg tggtgccgcg cggcttgccc aggatgtcca
     2221 cgacccgctc gcgggagtcg ccgacggcca cctccggcgc ggcgggggag tggtcctcgg
     2281 gccggttctt ccagcggaac accgcgtaca ccacgaccgc cgcgatgacg aggatggcca
     2341 gcagcaggag cgcgatgttc gggccgctgc ggggccagtt ttcatcgggg tcgggcgcgg
     2401 ggttcccggc ggggtcttga ggcggtgccg taaaatgatc catggctgct cccgttcgta
     2461 tctgtcgaaa tcctaggggc cggacttcct gccgtccagc ctaaagcccg cgctttttgt
     2521 ttcgcgcccg ccctttcttc cgctataaac ggcggcgcga aaggaatccg tatggccctg
     2581 tcactgtatc tgatcggcgc gccccgctcg gggaagacca ccgtgttcga ggcgctgacg
     2641 aacacgccgg aaggccggca tttcagcgcg aagggcgcgc atcacctcgg catggtcaag
     2701 gtgcccgacg cgcgcctggc cgcgctccgg gacatgtacc agcccgagaa atacacgccc
     2761 gcggaggtgt ccttcgtgga cgtggccctg ccgccggtcc ccgaaggagg ccacgccctg
     2821 tcgtcgctgg cggccctgct cggcgaggcc gacgcgttcg tgctggtcgt gcaggccttc
     2881 ggcgacatgg acggccgcgg gaagccgctg gacccggccg cgcagctgga atccacggcg
     2941 ctcgaaatcg tgttcgccga cctggaaaag gtcgagaccc ggctcgggcg gatcgagcac
     3001 gagaagaaac gcggcctgaa aaccaatccg gccgaggagg ccgccctgca gaagggcaag
     3061 gcggccctcg aggccgggac gccgctgcgc cgggtcgggt ggaacgcgga agaagagaag
     3121 acgctgcgca cgttccagtt cctttccatg aagccggccc tggtcatcgc caacacgggg
     3181 gagggggacc tcaaggggga gacgctgggc gcgttgcggc aggcggcgga ccgcctcggc
     3241 tgcgaaacgc tcgcgttctg cgccgcgctc gaggccgaga tggcccagct cgaagcgtcc
     3301 gcgcaggccg ctttcctgaa ggactatggc ctggaagagc cggcccgcat ccgcctgatc
     3361 caggccgcct accggctcct caacctgatc agtttcttca ccgtgggcga ggacgaagta
     3421 cgggcctgga cgatccgccg cggcacgcat gcccaggccg ccgccggcaa ggtgcacagc
     3481 gatatcgagc gcggcttcat ccgggccgag accgtggcca gcgcggaact gctgaaaacg
     3541 aagtcctggg cggcctgccg cgacaacgcc acgctccggc tggaaggcaa gacctacgag
     3601 gtccaggacg gcgacgtgat ccacttccgg ttcaacgcgt agtccggcgg ctttacgtcc
     3661 cgatcgagaa catggtctcg aggtcgtgct tggaatagac ctggaaggcg attagggtct
     3721 gcgagcgggt gatgccccgg accttgagca ggtgcttggt caccaggtcc gccatgccct
     3781 cgttgtcctt gacccggatg atggcgacca ggtcgtactg gccggcgacc gagtagacct
     3841 ccgagatccc ggcctggccg gccaattgct ccgcgacctc gttgaccttg tcccgatcca
     3901 cgttcagcag aaccagcgcg gtaaccatgg tgttgcctcc tcccgggttt ttcctgtatc
     3961 ctgtgcccag catagcacac acgcgcgtgt agaccaagcc ttggagccgc catgcgaagc
     4021 ctgcggatca agacgtacgc ggccggcagc gagaaaccga agtcggcgat cacgatcccc
     4081 ctggtcgtcg cccgcttggc cggcaaactg atcccgcgcg ccgtcgccga ggacctggcc
     4141 cgcaagggca tcaacgtcgt cgagatcctg gatatcatcc ggaaggaagg gctcgaaggc
     4201 accttgatgg agatcgagaa gggcggcgag cgaaccgtgg tctcggtgga ataaacttcc
     4261 accattcacg attcaccctt cacttcttct ccgccccgtc ctgcggcagc acgaggttca
     4321 gcaggatgcc gacgatgccc gccaggccga tgccgcccat cgcgaaccgg ccgcagctga
     4381 cctggaggtc gccgatgcca acgaccagga tcaccgaggc gatcacgagg ttacgcggct
     4441 tgctcatgtc cagctgggcc ttgaccagcg tcccggcgcc gatggccgcg atcatgccga
     4501 acagcagcac catgatgccg cccatcacgg gggaggggat ggtgcggagg aatccgccga
     4561 gcttgctcac gaaggccacc agcatggccg tcacggcggc gatgcgcatg tatttgggtt
     4621 cgaaggcccg ggtcagggcc accgccccgg tcacctccga gtaggtcgtg ttcggcggcc
     4681 cgcccagcag cgcggccaga gaggtggcga ggccgtcgcc cgccagcgtg cggtgcagcc
     4741 cgggatc
//
LOCUS       DAOOTK010000185         4627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_11688_length_4627_cov_4.448381, whole genome shotgun sequence.
ACCESSION   DAOOTK010000185 DAOOTK010000000
VERSION     DAOOTK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11688_length_4627_cov_4.448381"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PK634_13850"
     CDS             <1..410
                     /locus_tag="PK634_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOW98860.1"
                     /translation="IRDPDIENPDSYVRYLAEYIASQRIGIEVCLTSNLQTTPSIESV
                     GNHPLRKMIEHGLSVCLNTDNRLVSNTTVTRELKLAADHFPMTPHQFRNLVIAGFKGS
                     FFPGSYSAKRAYVRQVIRRYEALEKELLGAASD"
     gene            567..2057
                     /gene="guaB"
                     /locus_tag="PK634_13855"
     CDS             567..2057
                     /gene="guaB"
                     /locus_tag="PK634_13855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOW98861.1"
                     /translation="MSRNPHLDSFMAAFPREGLTFDDVCLVTRYADFLPTDTDLSAPF
                     SARIRLNIPFASAAMDTVTEARMAIAMAMLGGIGIIHRNLSVEEQARIVRTVKGHLNG
                     LIAQPVTFRVTDTLRTVRETRQARGYAFSGFPILDENDRLAGILTSADIKFARDPDAP
                     VADIMTRRVITAPAGTTLQQAYDLMQREKIGKLPLVESGRLVGLYSYTDVRTLIEKAE
                     PQFNRDAQHRLRVGAAIAPGDRERVERLAEHGADAVVIDTAHGHSQAVLEMVRWVKAG
                     FPALDVVAGNIATGEAARALQDAGADAVKVGIGPGSICTTRVVTGVGVPQLTAIYECA
                     RAVEGRIPVIADGGIRHSGDVPKAIAAGADTVMMGSVLAGTEESPGEKILYQGRQYVG
                     YRGMGSLGAMQAGQGSRKRYSQEHVNPDDLVPQGIEGMVPYAGTVQKVLTQYAGGLRL
                     ALGYAGCRSLRELQERGEFIRVTPAGAAEGHAHDVTITKEAPNYRT"
     gene            complement(2070..2381)
                     /locus_tag="PK634_13860"
     CDS             complement(2070..2381)
                     /locus_tag="PK634_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98862.1"
                     /translation="MTSLCSKTLCVWLVAGLLLAGVPLVGEAAISDSSSEGAVITVGL
                     FVTIVAVLFIVGFKSDMDNVFGQRAGRANLALSAEDDASVLEHEEAAAGSGGLGFRLA
                     F"
     gene            complement(2395..3285)
                     /locus_tag="PK634_13865"
     CDS             complement(2395..3285)
                     /locus_tag="PK634_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOW98863.1"
                     /translation="MKRSRLQETSIEVTVGAFMFMVLLALGFFTIVLSRENLFSRRYE
                     LDVVFQHVLGLRQGDNVFVRGVDIGKVKALHITPEGVHVLASLEQPVTLREDYKIEIL
                     PSSVLGGRYLGVFEGSEDRPLLPEGSEVRGVTPVDLLDEATRTVQLIKKGVEEGKIIE
                     NISAAADGVRKLMARLEEGEGTLGKLLTDETLYKNLQDISANLKDITARVNEGKGTVG
                     RLLSEDDTLYRDLSDAVAAVKDVTTSISEGDGTLGKLTRDDELYEQAKLLLNDIRAAI
                     DDLRETTPVTTFTSVFFGAF"
     gene            complement(3282..4037)
                     /locus_tag="PK634_13870"
     CDS             complement(3282..4037)
                     /locus_tag="PK634_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW98864.1"
                     /translation="MIRFEKVTRRLGGRLVLDAVDFEIEKGETFVIVGSSGAGKSVTL
                     KHMVRLLTPDAGRVWVGDDAVSEARGAELERIRSRFGVLFQGAALLQWLNVGDNVALP
                     LREHTDLGEEEIDRLVEERLRLVNLEDVAAKFPADLSGGMRKRVGLARAIVMKPEIIL
                     YDEPTSGLDPVTSRTIDHLIDNLRRELGVTSVVVTHDLHSALAIGTRIAMLHEGKIIE
                     VSTPEEFIRSKQEVVRGFLDAQYITQRGTWETA"
     gene            complement(4034..>4627)
                     /locus_tag="PK634_13875"
     CDS             complement(4034..>4627)
                     /locus_tag="PK634_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOW98865.1"
                     /translation="EALEKGVVTPEQTGIELRKFNQEVNIGTAVMVSMLREMGPFMTG
                     LIIAASVGSAIAAQIGTMAVSEEIDALELMSINPVKFLVTPRLIALAVMMPILTVYTN
                     ILGIVGGAIVGATQLGVSLTAYFDNATRYAENKDLYVGLFKAVLFGIIITTVACHQGF
                     STSEGAVGVGRSTRKTVIISFLTILVVGYMVTRLFYS"
BASE COUNT          763 a         1607 c         1530 g          727 t
ORIGIN      
        1 tgatccgcga cccggacatc gagaatccgg attcctacgt gcgctacctg gcggaataca
       61 tcgccagcca gcgcatcggc atcgaggtct gcctgaccag caacctgcag accacgccgt
      121 ccatcgaatc cgtgggcaac catccgctgc gcaagatgat cgagcacggc ctgtcggtgt
      181 gcctgaacac cgacaaccgg ctggtctcca acaccacggt cacccgcgag ctcaagctgg
      241 cggcggacca cttcccgatg acgccccacc agttccgcaa cctggtgatc gcgggcttca
      301 agggcagctt cttccccggc agctacagcg ccaagcgggc ctacgtccgg caggtgatcc
      361 ggcgctacga ggcgctggag aaggagctgc tgggcgcggc gtcggactga tcgctggcgc
      421 ggccggccca acgcgcggcg ccccggagcg taacaatttc attcacatcc gtgaatggat
      481 ttgttatcgc cgtgccggcg cgcattttcg ccattgacac ttcccctctt tcgcgtcatg
      541 gtccgcgggc caaaaggagc cgcgcgatga gccggaaccc gcacctcgac agcttcatgg
      601 ccgccttccc ccgcgaaggc ctgacgttcg acgacgtctg cctcgtcacc cgctacgcgg
      661 atttcctgcc gaccgacacc gacctctcgg cgccgttcag cgcgcgcatc cgcctgaaca
      721 tccccttcgc cagcgccgcc atggacaccg tcaccgaggc ccggatggcc atcgcgatgg
      781 ccatgctggg cggcatcggc atcattcacc gcaacctctc cgtcgaggag caggcccgca
      841 tcgtccgcac cgtgaagggg catctcaacg gcctgatcgc ccagccggtg accttccgcg
      901 tcacggatac gctccgcacc gtccgcgaga cgcggcaggc ccggggctac gccttcagcg
      961 gcttcccgat cctcgacgaa aacgaccgcc tggccggcat cctgacctcg gccgacatca
     1021 aattcgcgcg cgacccggac gcgccggtgg cggacatcat gacccgccgg gtgatcacgg
     1081 cgccggcggg cacgacgttg cagcaggcct acgacctgat gcagcgggag aagatcggca
     1141 agctgccgct ggtcgagagc ggccggctcg tcgggctcta ctcctacacc gacgtgcgga
     1201 cgctgatcga gaaggccgag ccgcagttca accgcgacgc gcagcaccgg ctgcgggtcg
     1261 gcgcggcgat cgcgcccggc gaccgggagc gggtcgagcg gctggcggag cacggcgcgg
     1321 atgccgtggt catcgacacc gcgcacgggc actcgcaggc cgtgctcgag atggtccgct
     1381 gggtcaaggc cgggttcccg gccctcgacg tcgtggccgg caacatcgcc acaggcgagg
     1441 cggcgcgcgc cctgcaggac gccggcgcgg acgccgtcaa ggtgggcatc ggcccgggct
     1501 cgatctgcac gacgcgcgtc gtcaccggcg tcggggtccc gcagttgacc gcgatttacg
     1561 aatgcgcgcg cgccgtggag ggacgcatcc cggtgatcgc cgacgggggc atccgccatt
     1621 ccggcgacgt gcccaaggcg atcgcggccg gggccgacac ggtgatgatg ggctccgtcc
     1681 tcgcgggcac ggaggagagc cccggcgaga aaatcctcta ccagggccgg cagtacgtcg
     1741 gctaccgggg catgggcagc ctcggggcca tgcaggcggg ccagggcagc cgcaagcgct
     1801 acagccagga gcacgtgaat cccgacgatc tcgtcccgca gggcatcgag ggcatggtcc
     1861 cctacgcggg gacggtgcag aaggtgctga cgcaatacgc cggcgggctt cgcctggccc
     1921 tgggctacgc gggatgccgc tcgctgcggg aactccagga gcggggcgaa ttcatccggg
     1981 tcaccccggc cggcgccgcc gaggggcacg cgcacgacgt gaccatcacg aaggaagcgc
     2041 cgaactaccg gacctgaccg tcccggcggt cagaacgcga gacggaatcc caggccgccg
     2101 gagcccgcgg ccgcctcctc gtgctccagc acggaggcgt cgtcctccgc ggacagcgcc
     2161 aggttggccc ggccggcccg ctggccgaac acgttgtcca tgtcggactt gaacccgacg
     2221 atgaagagga cggcgacgat cgtgacgaac aggccaacgg tgatcacggc gccctcggag
     2281 ctgctgtcgg agatggccgc ctcgccgacg agcgggacgc ccgcgaggag caggcccgca
     2341 accagccaga cgcacagggt tttgctgcac agcgatgtca tggcggttct cccgttagaa
     2401 ggccccgaaa aatacgctgg tgaaggtggt gaccggcgtc gtctcccgca ggtcgtcgat
     2461 ggccgcgcgg atgtcgttca gcagcagctt ggcctgctcg tagagttcgt cgtcgcgcgt
     2521 cagcttgccc agggtgccgt ccccctcgga gatcgaggtg gtgacgtcct tcaccgcggc
     2581 cacggcatcg gagaggtccc ggtagagggt gtcgtcctcg gagagcaggc ggccgacggt
     2641 gcccttgccc tcgttgacgc gggcggtgat gtccttgagg ttggccgaga tgtcttgcag
     2701 gtttttatag agggtctcat ccgtcagcag cttgccgagc gtcccctcgc cctcctcgag
     2761 ccgggccatc agcttgcgca cgccgtccgc ggccgcgctg atgttctcga tgatcttgcc
     2821 ctcctcgacg cctttcttga tgagctgaac ggtccgcgtg gcctcgtcga gcaggtccac
     2881 cggcgtcacg ccgcggacct cggaaccctc gggcaggagg ggccggtcct ccgagccctc
     2941 gaagacgccg aggtaccgcc cgccgaggac ggaggacggc aggatctcga tcttgtagtc
     3001 ctcgcgcagc gtcacgggct gctccaggct ggccagcacg tgcacgccct ccggcgtgat
     3061 gtgcagggcc ttgaccttgc cgatgtccac gccccggacg aagacgttgt ccccctgccg
     3121 caggcccagc acgtgctgga agacgacgtc cagctcgtag cgccgggaga acaggttctc
     3181 gcggctcaac acgatggtga agaagcccag cgccagcagg accatgaaca tgaaggcgcc
     3241 caccgtcact tcgatcgagg tttcctgcaa tcggctgcgt ttcatgctgt ttcccaggtc
     3301 ccgcgctgcg tgatgtactg cgcgtccagg aagccgcgca ccacttcctg cttggaccga
     3361 ataaactcct ccggcgttga aacttcaata attttcccct cgtggagcat ggcgatgcgc
     3421 gtcccgatgg ccagggcgct gtgcaggtcg tgggtcacca cgacgctggt gacgccgagt
     3481 tcccggcgca ggttgtcgat caggtggtcg atcgtgcggg aggtcacggg gtccaggccc
     3541 gacgtcggct cgtcgtagag gatgatctcg ggcttcatga cgatcgcccg ggccaggccc
     3601 acgcgcttgc gcatgccgcc ggacaggtcg gccggaaact tcgccgcgac gtcctcgagg
     3661 ttgaccagcc gcagcctctc ctccacgagc cggtcgatct cctcctcgcc gagatccgta
     3721 tgctcccgca ggggcagcgc gacgttgtcg ccgacgttga gccattgcag gagcgccgcg
     3781 ccctggaaga gcacgccgaa gcgcgagcgg atccgctcca actcggcccc gcgcgcctcg
     3841 ctgaccgcgt cgtcgccgac ccagacgcgg ccggcgtcgg gggtcagcag gcgcaccatg
     3901 tgcttgagcg tgacgctctt gcccgcgccg gaggacccga cgatcacgaa ggtctcgccc
     3961 ttctcgatct cgaagtccac cgcgtcgagc accaggcgcc cgccgagccg ccgcgtgact
     4021 ttttcgaagc ggatcatgag tagaacagcc tcgtcaccat gtaccccacc accaggatcg
     4081 tcaggaagga gatgatcacc gtcttgcgcg tggaccggcc gacgccgacg gcgccctcgg
     4141 aggtcgagaa cccctggtgg caggccacgg tcgtgatgat gatcccgaac aggacggcct
     4201 tgaagaggcc gacgtagaga tccttgttct cggcgtagcg cgtcgcgttg tcgaagtagg
     4261 ccgtcagcga gaccccgagt tgggtggccc cgacgatcgc cccgccgacg atgccgagga
     4321 tgttcgtgta gaccgtcagg atcggcatca tcacggccag cgcgatcagg cgcggcgtga
     4381 ccaggaactt tactgggttg atggacatca gctccagcgc gtcgatctct tccgagaccg
     4441 ccatcgtgcc gatctgggcg gcgatggccg agccgacgct ggcggcgatg atcaggccgg
     4501 tcatgaacgg gcccatctcg cgcagcatcg agaccatgac cgccgtcccg atattcactt
     4561 cctggttgaa cttgcgcagt tcgatccccg tctgttccgg cgtgaccacg cccttttcca
     4621 gcgcctc
//
LOCUS       DAOOTK010000186         4553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_11933_length_4553_cov_4.470654, whole genome shotgun sequence.
ACCESSION   DAOOTK010000186 DAOOTK010000000
VERSION     DAOOTK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11933_length_4553_cov_4.470654"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PK634_13880"
     CDS             <1..1979
                     /locus_tag="PK634_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOW98866.1"
                     /translation="MLNSTAPTTKEGIEKYLASGLIKGIGPIYAGKLVKAFGREVLNI
                     IESQSARLEDVEGIGPVRRARIKAAWNEQKAVREIMTFLMSHKVSTSRAFRIYKTYGD
                     AAIERVRKDPYCLARDIHGIGFKTADQIAASLGVEPTSPLRARAGVEYVLLELTDEGH
                     CAFPRGGLVERAATLLEIPAEILDRAVAEGLDSGRLVAGQDRFGEPLVYLAALDQAER
                     QLARQLVELARGAHPCPPIDMTKALPWVQDRIRMELSPEQADAVRLAVERKVMIITGG
                     PGVGKTTLVNAILKILRAKKLKVVLCAPTGRAAKRMTETTGLTAKTIHRLLEFDPGTG
                     GFRHDPAHPLSGDVFIVDETSMIDVLLANQLVRAVPRHAALILVGDVDQLPSVGPGCV
                     LRDLIDCNQFPACRLTHIFRQAASSAIVTNAHRVNTGELPHYPKEKTTDGPVSDFYFI
                     EADEPAAGCDRVVNLVRDAIPRRFQIRPQDIQVITPMQRGELGARNLNLRLQAALNPK
                     GPAIERYGWTFRVGDKVMQMENDYDKDVFNGDIGRITGLDEVEREIKVRFDDREVTYD
                     FQEMDEVSLAYATTIHKSQGSEYPCVVIPIHTQHYILLQRNLLYTAITRGRKLVVLVG
                     TVKAMAIAAHKVESHTRVTTLKDRLLEYAGAQMG"
     gene            complement(2079..2822)
                     /locus_tag="PK634_13885"
     CDS             complement(2079..2822)
                     /locus_tag="PK634_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOW98867.1"
                     /translation="MLQQTFLHLPGIGETRERRFWREGIRDWTAFLDAHGCGRLCGNR
                     YDRVAPTVEESIRRYAAGDWKHFEHCLPSAHKWRALGDLAGQALYVDIETNGYVGPDA
                     ITVIGVYDGREARAFVAERDLDKAVEVIEAHPLVVTFNGAAFDMPLIRQRFRHNLFNH
                     IHLDLRFPLKKIGFSGGLKKIEQAFGIERSERTRGLDGWDAVRLWNEYLEGYSPSLDV
                     LVEYNLEDVKNLEPLARYVFEQMKERIRI"
     gene            complement(2874..3512)
                     /gene="leuD"
                     /locus_tag="PK634_13890"
     CDS             complement(2874..3512)
                     /gene="leuD"
                     /locus_tag="PK634_13890"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOW98868.1"
                     /translation="MKAFSTHRGIVAPLDNANVDTDQIIPKQFLKSIKRTGFGEALFF
                     DWRYLPDGRPNPEFPPNRPEFKGASILVTRNNFGCGSSREHAVWAVMQYGFRVVIAPW
                     RQQGDARVPAFADIFRNNCVQNGLLTVELSEAEVEEIFQMVGRFPGLEATVDLDEQRV
                     VLHVQEEISFHFQIDPGVKEHLRRGLDAIGLTLQHEADIATFEKSHDVQMAR"
     gene            complement(3529..>4553)
                     /locus_tag="PK634_13895"
     CDS             complement(3529..>4553)
                     /locus_tag="PK634_13895"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009561651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOW98869.1"
                     /translation="DSHTSTHGAFAALAFGIGTSEVEHVLATQTLRQKKPKTFRVEYT
                     GRLKPGATSKDMILKLIGLIGTAGGTGYVLEYAGSAVSALSMEARMTIGNMSIEAGAR
                     AGMIAPDDTTFQYIASGDRPFAPRGAALDKAVAFWKTLPSDPGAKFDRELTIDASAIA
                     PQVTWGTSPGMVTDVSSVVPAPGKVPGYSAKDVEQALAYMGLTPGMRMTDIKVDTVFI
                     GSCTNGRIEDLRRAAAILKGRKIAKNVKAMVVPGSAAVRRQAMAEGLDKIFVAAGAEW
                     RYAGCSMCLAMNPDQLKPGERCAATSNRNFEGRQGKGGRTHLCSPEMAAWAAVEGHFV
                     DIRERM"
BASE COUNT          742 a         1575 c         1484 g          752 t
ORIGIN      
        1 agatgctcaa cagcacagcc ccgacgacga aagagggcat cgagaaatac ctcgcgtcgg
       61 gcctgatcaa gggcatcggg ccgatctacg ccggcaagct ggtcaaggcc ttcgggcgcg
      121 aggtgctgaa catcatcgag agccagtccg cccggctgga ggacgtcgag ggcatcgggc
      181 cggtccgccg cgcgcggatc aaggcggcgt ggaacgagca gaaggccgtc cgcgagatca
      241 tgaccttcct gatgagccac aaggtcagca cgtcccgggc gttccgcatc tacaagacct
      301 acggcgacgc cgccatcgag cgcgtccgga aggaccccta ttgcctcgcc cgcgacatcc
      361 acggcatcgg gttcaagacg gccgaccaga tcgccgcgag cctgggcgtg gagccgacgt
      421 cgccgctgcg cgcccgggcg ggcgtggagt acgtgctgct ggagctgacc gacgagggcc
      481 actgcgcctt cccccgcggc gggctggtcg agcgggctgc aacgctcctg gaaatcccgg
      541 cagagatcct cgatcgcgcc gtggccgagg ggctggacag cggccgcctc gtcgccggcc
      601 aggaccggtt cggggagccg ctggtctacc tcgccgcgct ggaccaggcc gagcggcagc
      661 tggcccggca actggtcgag ctcgcgcgcg gcgcccaccc ctgccctccg attgacatga
      721 ccaaggccct gccgtgggtc caggaccgca tccgcatgga gctgtcgccc gagcaggccg
      781 acgccgtgcg gctggccgtt gagcgcaagg tgatgatcat caccggcggg ccgggcgtgg
      841 gcaagacgac gctggtcaac gccatcctga aaatcctgcg ggccaagaaa ctcaaggtcg
      901 tgctctgcgc gccgaccggc cgcgccgcca agcggatgac cgagaccacc gggctgacgg
      961 ccaagaccat ccatcgcctc ctggaattcg acccggggac cggcggcttc cggcacgacc
     1021 ccgcgcaccc gctgtccggc gacgtcttta tcgtggacga aaccagcatg atcgacgtcc
     1081 tgctcgccaa ccagctcgtc cgcgcggtcc cgcgccacgc cgccctgatc ctggtcggcg
     1141 acgtggacca gttgccgtcg gtcggccccg gctgcgtcct gcgcgacctg atcgattgca
     1201 accagtttcc cgcctgccgc ctgacgcata tcttccggca ggccgccagc agcgccatcg
     1261 tcaccaacgc gcaccgggtt aacaccgggg aacttccgca ttatcccaag gagaagacga
     1321 cggacgggcc ggtttccgat ttctatttca tcgaggccga cgagccggcc gccggctgcg
     1381 accgcgtcgt gaatctcgtg cgcgacgcca tcccgaggcg attccagatc cggccgcagg
     1441 acatccaggt catcaccccg atgcagcgcg gcgaactggg cgcgcggaac ctcaacctcc
     1501 gcctccaggc tgcgctcaat cccaagggcc cggcgatcga gcgctacggc tggaccttcc
     1561 gcgtcggcga caaggtcatg cagatggaga acgactacga caaggacgtc ttcaacggcg
     1621 acatcgggcg catcaccggc ctcgatgagg tcgagcggga aatcaaggtg cggttcgacg
     1681 accgcgaagt gacctacgat ttccaggaga tggacgaagt atccctcgcc tacgcgacga
     1741 cgatccacaa gagccagggc agcgagtatc cctgcgtcgt gatcccgatc cacacgcagc
     1801 actacatcct gttgcagcgc aatctattat acaccgccat cacgcggggc cgcaagctgg
     1861 tcgtgctggt cggcaccgtg aaggccatgg ccatcgccgc gcacaaagtc gagtcccaca
     1921 cgcgcgtgac cacgctgaag gaccggctcc tcgaatacgc cggggcgcag atgggttgaa
     1981 ccacggaggc cacggaggtt cgcagaggac ggggacgcaa aatggcccat gaggtttccg
     2041 cttgaggcct tcctccgtgt cctctgcgac ctctgtggtt aaatccggat ccgttccttc
     2101 atctgctcga atacatatcg ggcgagtggc tccaggtttt tcacgtcttc caggttgtac
     2161 tcgacgagga cgtccagtga tggggagtag ccttccaggt attcgttcca cagccgcacg
     2221 gcgtcccagc cgtccagccc gcgcgtgcgc tcggaccgct cgatcccgaa ggcctgctcg
     2281 atcttcttga gcccgccgct gaagccgatc ttcttcagcg ggaaccggag atcgagatgg
     2341 atatggttga acaggttgtg ccggaaccgc tggcggatca ggggcatgtc gaaggccgcg
     2401 ccgttgaacg tgacgaccag cgggtgggcc tcgatgacct cgacggcctt gtccagatcg
     2461 cgctccgcca cgaaggccct cgcctcccgg ccgtcgtaca cgccgatgac cgtgatcgcg
     2521 tccgggccga cgtacccgtt ggtctcgatg tccacgtaga gagcctggcc ggccaggtcg
     2581 cccagcgccc gccacttgtg cgcggacggc aggcagtgct cgaagtgttt ccagtcgccg
     2641 gcggcgtacc gccggatgga ttcctccacg gtcggcgcca cacggtcata gcggttcccg
     2701 cagaggcgcc cgcatccgtg ggcgtccaga aacgccgtcc agtcgcggat cccctcccgc
     2761 cagaaccgcc gctcgcgggt ctcgccgatg ccgggcaagt ggaggaaggt ttgctgaagc
     2821 atgggcgtag gggcgctgct tgcagcgccc gtgggcgcct tcaagcggcg cccctaccgg
     2881 gccatctgca catcatggct tttttcgaac gttgcgatgt ccgcctcgtg ctgcagcgtc
     2941 agcccgatcg cgtccaagcc gcggcgcagg tgctccttca cgccgggatc gatctggaaa
     3001 tggaaggaaa tctcctcttg cacgtgcagc acgacgcgct gctcgtccag gtcgaccgtg
     3061 gcctccagcc ccgggaaccg gccgaccatc tggaagatct cctcgacctc ggcctcggac
     3121 agctcgacgg tcagcaggcc gttctgcacg cagttgttcc ggaagatgtc cgcgaacgcg
     3181 ggtacgcgag cgtcgccctg ctggcgccac ggcgcgatga caacgcggaa gccgtactgc
     3241 atcacggccc agaccgcgtg ctcgcggctg gagccgcagc cgaagttgtt gcgggtgacc
     3301 aggatggagg cccccttgaa ctcgggccgg ttgggcggga attccgggtt gggtctcccg
     3361 tccggcaggt agcgccagtc gaagaacagg gcctcgccga atccggtccg cttgatcgac
     3421 ttcaggaatt gcttcgggat gatctggtcc gtgtccacgt tggcgttgtc cagcggggcc
     3481 acgatgccgc gatgcgttga aaatgctttc atgcgcgggt ttccttcctc acatccgttc
     3541 ccggatatcc acgaaatgac cttcgacggc ggcccaggcg gccatctcgg ggctgcacag
     3601 gtgcgtgcgc ccgcccttgc cctgccgtcc ctcgaagttg cggttcgacg ttgcggcgca
     3661 gcgttcgccg ggcttcagct ggtccgggtt catcgccagg cacatgctgc agcccgcgta
     3721 gcgccactcc gcgccggcgg cgacaaagat cttgtccagc ccctccgcca tggcctgccg
     3781 gcgcacggcg gccgagccgg gcacgaccat cgccttcacg ttcttcgcga tcttccgccc
     3841 cttcaggatg gcggcggccc tgcgcaggtc ctcgatgcgc ccgttggtgc agctcccgat
     3901 gaacacggtg tccaccttga tgtccgtcat ccgcatgccc ggcgtcaggc ccatgtaggc
     3961 cagggcctgc tcgacatcct tggcgctgta gccgggcacc ttgccgggcg ccgggacgac
     4021 gctgctgacg tcggtcacca tgccggggct ggtgccccag gtcacctgcg gcgcgatggc
     4081 cgaggcgtcg atggtcagct cgcggtcgaa cttcgcgccg gggtcgctgg gcagggtctt
     4141 ccagaacgcc acggccttgt ccagcgcggc gcccctcggc gcgaacggcc ggtctcccga
     4201 ggcgatgtac tggaacgtcg tgtcgtcggg cgcgatcatc ccggcgcgcg cgccggcctc
     4261 gatgctcatg ttgccgatgg tcatccgcgc ctccatggac agcgcggaca cggccgagcc
     4321 ggcatattcc aggacatagc ccgtgccccc ggccgtcccg atcaggccga tcagcttcag
     4381 gatcatgtcc ttcgacgtcg cgcccggctt caggcggccg gtgtactcga cccggaaggt
     4441 cttcggcttc ttctggcgca gcgtctgggt cgccaggacg tgctcgacct cggaggtccc
     4501 gatgccgaag gcaagcgccg cgaacgcgcc atgggtgctg gtatggctgt cgc
//
LOCUS       DAOOTK010000187         4480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_12212_length_4480_cov_4.548927, whole genome shotgun sequence.
ACCESSION   DAOOTK010000187 DAOOTK010000000
VERSION     DAOOTK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12212_length_4480_cov_4.548927"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PK634_13900"
     CDS             complement(<1..612)
                     /locus_tag="PK634_13900"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOW98870.1"
                     /translation="MQVAAISREELDAWPIAEAGLPVRVVNAVREVPVSTVGQLRQLP
                     DPDLLKLRSLGRISLGHIHAFFRICGQIEQGRQRFGTVQEVFALCLDDAERNVLAGRY
                     GLDRDPEKPSRRRMTLQQIADAGHITRERVRQIQDAGLRKLQSRIAQVCLQPFYDYAC
                     GQVEKRGGLAGGAELLGLRSEPAMGGLNPLGLMRLLSEVRSARL"
     gene            738..3872
                     /gene="ileS"
                     /locus_tag="PK634_13905"
     CDS             738..3872
                     /gene="ileS"
                     /locus_tag="PK634_13905"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOW98871.1"
                     /translation="MFDPVSSKVSFPDLERDILKFWEAGDVFRKSIEIRRGAPEFVFY
                     DGPPFATGLPHYGHLLAGTIKDIVPRYQTMRGHYVERRFGWDCHGLPVEYEMEQELKI
                     SGKRDIEKLGVDVFNEKCRGIVLRYTREWRQIVTRMGRWVDFDHDYKTMDPAYMESIW
                     WVFKSLWEKDLIYEGHKILPYCPRCATPLSNFETNQGYQDVQDPAVTVRFAVEGAANT
                     FILAWTTTPWTLPSNLALAVGPTIRYVKVRDAGQVYILARDRLPAYYPKNESYELLEE
                     LDGSALAGLRYEPLFPYFADLQAKGAFRVLEADFVSTEDGTGVVHIAPGFGEDDHRLG
                     QAAGLPAVCPVDDEGRFAAPVADYLGREVKETDSDILRRLKEEGKLVHRSTIQHSYPH
                     CWRCDSPLIYRAVATWFVRVEQLRDRLLAANAKTHWVPEHLRDGRFGKWLEGARDWAI
                     SRNRYWGAPLPVWRNAQGERICIGSVAELEKLSGQKVADLHKHFVDNVEIPSAKGGAP
                     LRRIPEVLDCWFESGSMPYAQSHYPFENKAHFEQHFPADFIAEGLDQTRGWFYTLMVL
                     STALFDKPTFRHVIVNGMILADDGRKMSKRLKNYPDPVYILDTYGADALRLYLIHSPV
                     VRAENLNFSEEGVKHALRHLLLPWWNAYSFFVTYARVDGWRPGKGSGFGGPGSGNLLD
                     NWIHSSLERLTQEVVAAMDAYDLQQAVRPFVRFIEDLTNWYIRRSRRRFWKSSDDADK
                     ARAYATLYEVLVRLCRIAAPFVPFISEAIYRNLRTPDMPESVHLCDFPLGDGRARNAA
                     LEDQMDDVIRVVGLGRQLRADHNIKVRQPLRGLHVVCRDAARRARIEALKDLVADELN
                     VREIWFSGRESDLATFLAKPDFRRMGPRLGPKVKKAAQQIQKLDSARIEDLLDGKPVS
                     IEVDGESVAIEAADLVVERKPREGLAVSSEDGLIVALEIDLTQELVREGLAREFVNRV
                     QNLRKAADLEVTQRIRLSYSGDEAVREAVEAHGDYIRGETLCVECLFVGSAGDVQDLN
                     GHPCGVRIEPAKEK"
     gene            3979..>4480
                     /locus_tag="PK634_13910"
     CDS             3979..>4480
                     /locus_tag="PK634_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98872.1"
                     /translation="MAKSAKKSKKRAPVQKKAPVKAKPVKKKAAPAAKARKPVPVRKP
                     VAARRRPAPKAAPEPKKPLPPPRRLPAVELKKYREVLQRLRDRVVDEISFLAGDNLNR
                     SPRESSGDLSSYSFHMADQGTDNFDREFALNLVSSEQDALYEIDEALQRITTGIYGSC
                     DNCGKPI"
BASE COUNT          787 a         1522 c         1462 g          709 t
ORIGIN      
        1 cagccgggcg gaccgcacct cgctgagcag ccgcatgagc cccaggggat tcagcccgcc
       61 catcgccggc tccgagcgaa ggccgaggag ttccgcgcct cccgccagcc cgccacgctt
      121 ctccacctgg ccgcaggcat aatcgtagaa gggctgcagg cagacctggg cgatgcggct
      181 ctgcaacttc cgcaggccgg cgtcctggat ctggcgcacc cgttcgcgcg tgatgtgccc
      241 cgcgtccgcg atctgctgga gcgtcatccg gcgccgcgag ggcttttcgg ggtcgcggtc
      301 caggccgtat cgccccgcga gaacgtttcg ctccgcgtcg tccaggcaca gcgcgaaaac
      361 ctcctggacc gtgccgaacc gctgccggcc ctgttcgatc tgcccgcaga tccggaaaaa
      421 cgcgtgaatg tgtcccagcg agatgcggcc caggctccgc agtttcagca aatccggatc
      481 ggggagctgc cgcagctggc ccaccgtcga aaccggcacc tcccggacgg cattgacaac
      541 gcgcaccggc agcccggcct ccgcgatggg ccaggcatcc agttcttcgc gggatatggc
      601 tgcgacttgc acaaaaagtc cttaatatgc gtggagaatg cggtcgttta cgatgaaatg
      661 tcaagtaata ttctccgcgt gcggggcccg catctccgct tgctttcggc aatcgcggtt
      721 gcgtatcttc tacgcccatg tttgaccccg tatccagcaa ggtgagcttt ccggacctcg
      781 aacgggatat cctgaagttc tgggaggcgg gcgacgtctt ccggaaatcc attgaaatcc
      841 gcaggggcgc gccggaattc gtcttctacg acggccctcc gttcgccacc ggcctcccgc
      901 actacgggca cctgcttgcc ggcacgatca aggacatcgt cccgcgctac cagaccatgc
      961 gcggccatta cgtcgagcgc cggttcggct gggattgcca cggcctgccc gtggaatacg
     1021 agatggagca ggagctcaag atcagcggca agcgcgacat cgagaaactt ggcgtcgacg
     1081 tcttcaacga gaaatgccgc ggcatcgtcc tgcgctatac ccgcgagtgg cgccagatcg
     1141 tcacccgcat ggggcgctgg gtggacttcg accacgacta caagaccatg gacccggcct
     1201 acatggagtc catctggtgg gtcttcaaat cgttgtggga aaaggacctg atctacgagg
     1261 ggcacaagat cctgccgtac tgcccgcgct gcgcgacgcc gctctccaac ttcgagacga
     1321 accagggcta ccaggacgtc caggacccgg ccgtcaccgt ccgcttcgcc gtcgagggcg
     1381 ccgcgaatac gttcatcctg gcctggacga cgacgccctg gacgctgccg tccaacctcg
     1441 ccctcgccgt cgggccgacg atccgctacg tgaaagtccg cgatgcgggc caggtctaca
     1501 tcctggcccg cgaccgcctg ccggcatact acccgaagaa cgaatcgtat gagttgctgg
     1561 aggaactgga cggctcggcc ctggccggcc tgcgctacga gccgttgttc ccgtacttcg
     1621 cggacctcca ggcgaagggc gcgttccgtg tcctcgaggc cgatttcgtc tccaccgagg
     1681 acggcaccgg ggtcgtgcac atcgcgcccg gcttcggcga ggacgaccac cggctcggtc
     1741 aggccgccgg cctgccggcc gtatgcccgg tggacgacga gggccggttc gccgcgcccg
     1801 tcgcggacta cctcggccgc gaggtgaagg agaccgactc cgacatcctg cgccgcctga
     1861 aggaggaggg gaagctcgtc caccgctcga cgatccagca cagctaccca cattgctggc
     1921 gctgcgattc gccgctgatc taccgcgcgg tggcgacctg gttcgtccgc gtcgagcagc
     1981 tccgcgaccg gctcctcgcg gccaacgcga agacccactg ggtgcccgag cacctgcgcg
     2041 acggccgctt cggcaagtgg ctcgagggcg cgcgggactg ggcgatctcg cgcaaccgct
     2101 actggggcgc gccgctgccc gtctggcgca acgcgcaggg cgaacggatc tgcatcgggt
     2161 ccgtcgccga actggaaaaa ctctccggcc agaaggtcgc cgacctgcac aagcacttcg
     2221 tggataacgt cgagatcccg tcggcgaagg gcggggcgcc cctgcgccgc atccccgagg
     2281 tgctggactg ctggttcgag agcggctcga tgccgtacgc ccagtcgcac tatcccttcg
     2341 agaacaaggc gcacttcgag cagcacttcc cggccgattt catcgccgag gggttggacc
     2401 agacgcgcgg ctggttctac acgctgatgg tcctttcgac ggcgctgttc gacaagccca
     2461 cgttccgcca cgtgatcgtc aacggcatga tcctggccga cgacggccgc aagatgagca
     2521 agcggctcaa gaactacccc gatcccgtct acatcctgga cacctacggc gccgacgcgc
     2581 tgcgactgta cctgatccac tcgccggtcg tccgcgccga gaacctcaat ttctccgagg
     2641 agggcgtcaa gcacgccctg cgccacctcc tgctgccgtg gtggaacgcc tacagcttct
     2701 tcgtcacgta cgcgcgggtg gacgggtggc ggccggggaa gggttcaggg ttcggggggc
     2761 cgggttcagg gaatctgttg gacaactgga ttcactcgtc gctggagcgc ctgacgcagg
     2821 aggtcgtcgc ggcgatggac gcctacgacc tgcagcaggc ggtccggccg ttcgtccggt
     2881 tcatcgagga cctgaccaat tggtacatcc gccgcagccg gcgccggttc tggaagagct
     2941 cggatgacgc ggacaaggct cgggcttacg ccaccctcta cgaggtgctg gtccggttgt
     3001 gtcggatcgc cgcgccgttc gtgccgttca tcagcgaggc gatctaccgc aacctgcgca
     3061 cgccggacat gccggagtcc gtgcacctct gcgatttccc gctcggcgac ggccgcgccc
     3121 gcaacgccgc gctggaggac cagatggacg acgtgatccg cgtcgtcggc ctcggccgcc
     3181 agctgcgcgc cgatcataac atcaaggtgc gccagccgtt gcgagggctg cacgtggttt
     3241 gccgcgacgc cgcgcgccgg gcgcggatcg aggcgctcaa ggacctggtc gcggacgaac
     3301 tcaacgtgcg cgagatctgg ttcagcgggc gcgagtccga cctggcgacc ttcctcgcca
     3361 agcccgattt ccgccggatg gggccgcgcc tgggcccgaa ggtgaagaag gccgcgcagc
     3421 agatccagaa gctggactcc gcccgcatcg aggatctgct ggacggcaag cccgtttcga
     3481 ttgaggtgga tggcgagtcg gtcgccatcg aagccgccga tctggtggtg gagcgcaagc
     3541 cgcgcgaggg cctggccgtg tcctcggagg acgggctgat cgtcgccctg gagatcgacc
     3601 tgacccagga actggtgcgc gaaggcctcg cccgcgagtt cgtcaaccgc gtccagaacc
     3661 tgcgcaaggc ggccgatctc gaggtgaccc agcgcatccg gctgtcgtac agcggcgacg
     3721 aagccgttcg cgaggccgtg gaagcgcacg gcgactatat acgcggcgag accttgtgcg
     3781 tagaatgcct gttcgtcggc tcggcggggg atgtccagga cctgaacggt catccctgcg
     3841 gcgtccggat cgagcccgcg aaggaaaaat aagtcggcag ggttgacagc ggcagagggc
     3901 tgtggtactc aagccgctct tccgccggac tggaccggcg gggacccgtc aaacaagacg
     3961 tcagggggaa ggtgcgagat ggcaaaaagc gcaaagaaat ccaagaaacg cgcgcccgtt
     4021 cagaagaagg cgccggtcaa ggccaagccc gtgaaaaaga aggcggcgcc cgccgccaag
     4081 gcgcgtaagc cggtcccggt tcgcaaaccg gttgccgcca ggcgcaggcc ggccccgaag
     4141 gcggcgccgg aaccgaagaa gccccttccg ccgccccgcc ggctgccggc cgtggagttg
     4201 aagaagtacc gcgaggttct ccagagactt cgcgaccgcg tcgtcgacga gatctcgttc
     4261 ctcgcgggcg acaacctgaa ccggtccccg cgcgagtcgt ccggcgactt gtccagctac
     4321 agcttccaca tggccgacca gggcaccgac aacttcgacc gcgagttcgc cctgaacctg
     4381 gtcagcagcg agcaggacgc cctctacgag atcgacgagg cgctccagcg catcacgacc
     4441 ggcatctacg gctcctgcga caattgcggc aagcccatcg
//
LOCUS       DAOOTK010000188         4442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_12366_length_4442_cov_4.826533, whole genome shotgun sequence.
ACCESSION   DAOOTK010000188 DAOOTK010000000
VERSION     DAOOTK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12366_length_4442_cov_4.826533"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PK634_13915"
     CDS             <1..736
                     /locus_tag="PK634_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98873.1"
                     /translation="KWKAEAEAARKAATEAEGRAGQAATALQAVQSQRAQAERERDEA
                     RHRAEDAEKRAVELEKKSETATTEARLALEQRAAAQAESASAAERVAIEIRNLQEQLL
                     RARQEKDEAKAAAASEIDKARIRAEQAAAEMAETKAIVQKQAAELRAAAAQLAAARAE
                     GEKWKDQCGRARATAAEAEKARMAALKPPDGLVPRALLDEAQRRIAHLERSYKQALQT
                     VQRSLSARAGESQAMPPDQLRRRDIA"
     gene            748..1266
                     /locus_tag="PK634_13920"
     CDS             748..1266
                     /locus_tag="PK634_13920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOW98874.1"
                     /translation="MKKQKYRIREAGFADAPAIFAIIKKHPDELVPRPVSDIIQNIDR
                     FLVAEADGAIVGNASWGILPEIGQAKHPTIEIKSVAVDRAHRGTGLGRALVQAAIRRV
                     KKLKPEMVIVLTFKPEFFRKFGFSEVPKESIMHKLYTGCVNCTKYDSPFTCPEVAMGL
                     KWKYDPPAPGGK"
     gene            complement(1274..2401)
                     /locus_tag="PK634_13925"
     CDS             complement(1274..2401)
                     /locus_tag="PK634_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98875.1"
                     /translation="MPPAPSAPDKNDPRLRQVTAAARALAGLISTARMYQVQHPAFQR
                     AVEQQLAALAAAMDEAGEVVLYFADQQIRHGALALEPESGLMRTTAKWFEDRGVKGIS
                     LLPGLTAAELASFAGIMLSTDRNLETSGLTLCLEHAGIRHILEQKVKYGIIGRERVVY
                     PPTAGSGPAAKAGSTPPAPVAAKTAAPTAAKTPTPAGASLKSWDLEAETTAGTRAVPP
                     RLAGQKAVSSARRPLRDFVHSVFDAVSKNEATPPQAADIIAAEIETRIEEKADEVRAE
                     QEGRIRRLEQVKDVLLEELDDLHLAALLIDPERRVVAMNKAARDLLGLIDKLQDGSPL
                     DQFIRSGKERQTVELPGASRLAHMIIAPDAGEGNLMLVCLE"
     gene            2544..3302
                     /locus_tag="PK634_13930"
     CDS             2544..3302
                     /locus_tag="PK634_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOW98876.1"
                     /translation="MTKLERLVAALDRELRLDRFRDSSRNGLQVANDGRVRKIACGVD
                     ANLEFFEAARRRGADLLICHHGLSWGDSMTRLTGAQYRRVAFLVRNNMALYAAHLPLD
                     AHPRLGNNAQIARAMGLRGLKPFGNYHGQVIGRSGRLARPMTLAAFKKRVKRVMRHGL
                     QTMEYGPAVVRTVAVVSGGAADLVEEAGREGIDVFLSGEPALQARTSAMEYGVHAVFA
                     GHYATELFGVRAVGGYLARRFRVPCETLDFRVPF"
     gene            3400..>4442
                     /locus_tag="PK634_13935"
     CDS             3400..>4442
                     /locus_tag="PK634_13935"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOW98877.1"
                     /translation="MSNKFYELAQQQRVALTRFMQDIVSIPSLSSQEGAVVKRMAAEM
                     KKLGYDEVTIDPMGNLIGRVGHGPVKIAIDGHCDTVDVGNASLWKRDPFKAAVENGIL
                     YGRGASDQKGGVASAIYCAPLLKQAGFPEKATLYVVASVQEEDCDGLCWQYILKEDKI
                     RPDVVVLTEPTSLKIYRGHRGRMEIEVSTEGLSCHGSAPERGVNAVYKMAPIIQDIEK
                     LNERLPPRAPLGKGTVTISHIRSTSPSLCAVADSCTIHLDRRLTVGETEETSVKELYD
                     LPSVKAASAKVKVLEYAVPSFKGLTYPTKKYFPTWEFPESTPHVQKAIAAYRGTFGGE
                     PEVGHWTFSTNGIA"
BASE COUNT          783 a         1464 c         1533 g          662 t
ORIGIN      
        1 aaagtggaag gcggaagcgg aggccgcgcg caaggccgca accgaggcgg aagggcgggc
       61 tggccaggcc gccaccgccc tgcaggccgt tcagagccag cgggcgcagg cggagcggga
      121 gcgcgacgag gcgcgccacc gagccgagga cgccgagaaa agggccgtcg agctggaaaa
      181 gaaatcagag acagccacaa ccgaggcccg attggcgctg gagcaacggg ccgcggcgca
      241 ggcggaatcc gcgtctgccg cggaacgcgt cgccattgag atccggaacc ttcaggagca
      301 actgctccgc gcccgccagg agaaggatga agcgaaggcc gccgcggcct ccgagatcga
      361 caaagcccgg atccgtgccg agcaggcggc cgcggagatg gcagaaacca aggccattgt
      421 gcaaaagcag gccgccgaac tgcgggcggc cgccgctcaa ctggcggccg cccgggcgga
      481 gggcgaaaag tggaaggatc agtgcgggcg cgcccgggcg acggcggcgg aggcggaaaa
      541 agcccgcatg gccgccttga aaccaccgga cggcctggtg cctcgcgccc tcctggacga
      601 ggcgcagcgg cggatcgcgc acttggagcg cagttacaag caggcgctgc aaacggtaca
      661 gcgcagcctt tcagcgagag ccggggaaag ccaggccatg ccgcccgacc aactgcggcg
      721 gcgggatatt gcgtgagccg ggggagcatg aaaaaacaaa aatacaggat tcgggaggcc
      781 gggttcgcgg atgccccggc gatcttcgcc atcattaaaa aacacccgga cgaactggtc
      841 ccgcgcccgg tcagcgacat cattcagaac atcgaccgct tcctcgtggc cgaggccgac
      901 ggcgctatcg tcggcaacgc gtcgtggggc atcctgcccg agatcggcca ggcgaaacat
      961 ccgaccatcg agatcaagtc ggtcgccgtg gaccgcgcgc accgcggcac cggcctgggc
     1021 cgggccctgg tccaggccgc gatccgccgc gtgaagaagc tgaagccgga aatggtcatc
     1081 gtgctgacct tcaagccgga gttcttccgc aagttcgggt tttccgaggt gcccaaggag
     1141 agcatcatgc acaagctcta cacgggctgc gtgaactgca cgaagtacga ctccccgttc
     1201 acctgcccgg aagtcgcgat gggcttgaag tggaagtacg atccaccggc gccgggcggg
     1261 aagtaagccg ggcctactcc aggcacacca gcatcaggtt gccctcgccg gcgtcgggcg
     1321 cgatgatcat gtgcgcgagc cgcgaggccc ccggcaactc cacggtctgg cgctccttgc
     1381 cggagcggat gaactggtcc agcggcgagc cgtcctggag cttgtcgatc agccccagca
     1441 agtcgcgcgc ggccttgttc atggcgacga cccggcgctc cggatcgatc agcagggccg
     1501 ccaggtgaag atcgtccaac tcctccagca gcacgtcctt gacctgctcg aggcgccgaa
     1561 tgcggccttc ctgctcggcg cggacctcgt ccgccttctc ctcgatccgg gtctcgattt
     1621 ccgcggcgat gatgtccgcc gcctggggcg gcgtggcctc gttcttggaa acggcgtcga
     1681 agacgctgtg cacaaaatcg cgcagcggcc tgcgcgcgga cgatacggcc ttttgcccgg
     1741 ccaggcgcgg gggaacggct cgtgttcccg ccgtggtttc ggcttccagg tcccaggact
     1801 tgagcgaagc gccggcgggc gtcggcgtct tggcggcggt cggggcggcg gttttcgcgg
     1861 cgaccggcgc cgggggcgtc gagccggcct tggcggccgg gccgctgccg gcggtcggcg
     1921 gatacacgac gcgttcgcgg ccgatgatcc cgtacttgac cttctgctcc aaaatgtggc
     1981 ggatgccggc atgctcgagg cagagcgtca ggccgctcgt ttcgaggttc ctgtccgtgg
     2041 agagcatgat gccggcgaaa gaagccagtt ccgcagccgt caggccggga aggagcgaga
     2101 tgcccttgac gccgcggtcc tcgaaccact tggcggtggt ccgcatcagg ccggactcgg
     2161 gctccagcgc cagcgcgccg tgccggatct gctggtcggc gaaataaaga accacttcgc
     2221 cggcctcgtc catcgcggcg gcgagcgcgg ccagttgctg ctcgaccgcc cgctgaaagg
     2281 ccgggtgttg cacctggtac atccgcgcgg tgctgatcag gcccgccagc gcgcgcgcgg
     2341 cggcggtcac ctggcggaga cgcggatcgt ttttatcggg cgcgctgggt gccggcggca
     2401 tagggctctc cctctttcac aaaagatgaa tacttgtctt ttttcccatc cttgtaaagc
     2461 caaacgcgtg atcgccgggg gggaataatg ggcttgcagt ttggggcccc ggcgacaaga
     2521 tgccggcagt cccgaggtgc ctcatgacga aactggaaag actggtggcg gccctggatc
     2581 gcgaactgcg gctcgatcga ttccgcgatt cctcgcgcaa cggcctgcag gtcgccaacg
     2641 acggccgggt ccggaagatc gcgtgcggcg tggacgccaa cctggagttc ttcgaagccg
     2701 cccgccgccg gggggccgac ctgctgatct gccaccacgg cttgagctgg ggcgactcca
     2761 tgacccggct gaccggggcg caataccggc gggtcgcgtt cctcgtccgg aacaacatgg
     2821 ctctgtatgc cgcgcatctt ccgctggacg cccatccgcg gctcggaaac aacgcgcaga
     2881 tcgcccgcgc catggggctg cgcgggctga agccgttcgg aaattaccac ggccaggtga
     2941 tcggcaggtc cgggcgcctg gcgcggccga tgaccctcgc cgcgttcaag aagcgcgtga
     3001 agcgcgtcat gcgccatggc ctgcagacca tggagtacgg cccggcggtg gtgcggacgg
     3061 tcgcagtggt gtcgggcggc gcggcagacc tggtcgagga ggcggggcgc gaggggatcg
     3121 acgtcttcct gtccggcgag cccgccctgc aggcccggac ctcggcgatg gaatacggag
     3181 tgcatgccgt cttcgcgggc cactatgcca ccgagctctt cggcgtgcgc gcggtcggtg
     3241 ggtatctggc ccgccgcttc cgcgtcccgt gcgagacgct ggatttccgc gtgcccttct
     3301 gaccggtgcg cgccgcccgg ttgcgggaaa agcctttttt gacttgagcc cggccgggtt
     3361 gtggtgtttg ttccccctgt taaaccaaga aggaaaacca tgagcaacaa gttctacgag
     3421 ttggcgcagc agcagagggt ggccctgacg cggttcatgc aggacatcgt ttcgatcccc
     3481 tccttgagca gccaggaggg cgcggtcgtc aagcgcatgg ccgccgagat gaagaagctc
     3541 gggtatgacg aggtgaccat cgatcccatg ggcaacctga tcggccgcgt cggccacggc
     3601 ccggtgaaga tcgccatcga cgggcactgc gacaccgtgg acgtcggcaa cgcctcgctc
     3661 tggaagcgcg atcccttcaa ggccgcggtc gagaacggca tcctctacgg ccgcggcgcc
     3721 agcgaccaga agggcggcgt ggcctccgcg atctactgcg ccccgctcct gaagcaggcg
     3781 ggcttccccg agaaggccac gctctacgtc gtcgcctccg tccaggagga ggattgcgac
     3841 gggctctgct ggcagtacat cctcaaggag gacaaaatcc gtcccgacgt tgtcgtgctg
     3901 accgagccga ccagcctgaa gatataccga gggcaccgcg gccgcatgga gatcgaggtc
     3961 tccaccgagg gcctctcctg ccacggctcc gcccccgagc gcggcgtgaa cgccgtgtac
     4021 aagatggccc cgatcatcca ggacatcgag aagctgaacg agcgcctgcc gccccgcgcc
     4081 ccgctgggga agggcaccgt gaccatcagc cacatccgct ccacgtcgcc cagcctgtgc
     4141 gccgtcgcgg acagctgcac gatccacctc gaccgccgcc tgaccgtggg cgagacggag
     4201 gagacttccg tcaaggagct gtacgacctg ccctccgtca aggccgccag cgcgaaggtc
     4261 aaggtgctgg agtacgccgt gccgagtttc aagggcctga cctatccgac caagaaatac
     4321 ttcccgacgt gggaattccc ggaatccacg ccgcacgtcc agaaggccat cgcggcctac
     4381 cgcggcacgt tcggcggcga accggaggtc ggccactgga ccttcagcac taatggcatc
     4441 gc
//
LOCUS       DAOOTK010000189         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_12571_length_4386_cov_4.233664, whole genome shotgun sequence.
ACCESSION   DAOOTK010000189 DAOOTK010000000
VERSION     DAOOTK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12571_length_4386_cov_4.233664"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..967
                     /locus_tag="PK634_13940"
     CDS             125..967
                     /locus_tag="PK634_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOW98878.1"
                     /translation="MIDLHSHSIFSDGSLTPEELVDQACAAGLTAVALTDHDTTSGLP
                     RFLKAAGGRPLRAIPGVEISADFSPGAMHMLGYFIRPDDAALEERLVWIREGRDKRNA
                     EILEKLNALGFGIAWNEVAKHAGADVVGRPHFAQVLLERGIVKTKDEAFDLYLGKGKQ
                     AYAERRRFSTEDSIALIRAAGGVAVLAHPFTLGLDNGKLRELVQRLAGQGLGGLEVYY
                     SEHSANLQQAYMKLAQDFGLVATGGSDFHGALAPDIRLGSGFGGLRVPDEVVDKLEAR
                     RDYK"
     gene            complement(958..2742)
                     /locus_tag="PK634_13945"
     CDS             complement(958..2742)
                     /locus_tag="PK634_13945"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOW98879.1"
                     /translation="MDAGQAADVAVAAMGSRDIFFLTAMVLGGLVLFIQGMNMMSDAL
                     RKAAGSSMRTLLSSATRNRFGGLFLGLVLGTLIQSSATSVMIVGFINAGLMTLVESVG
                     VMLGMNIGTTISMQLVSFKLGAYCFFLITTGFVLQFVIPNDKVRHVGQSLLGFGIIFL
                     GLNIMSDAIKPHREMFTDFIQGIQGDTMPGLLKGVLISTLVTGIIQSSGAVIAMCFAL
                     ISAGAVTDLNQVYPVVLGANIGTCATGLLGSIGTNIEARRSAVSHLMFNVLGSLVAIV
                     FAPWFIRFGEWTPGDLIHQTANVNTAVMVVRALMVLPIPSAYARFITWLMPSRKPPPP
                     PTFLDRKLVQFPEKAIYAAICELRRVMKVCAESFSITIDVLFRPERKKVQAIKLNENV
                     VDEIKRSMKDYLGVLTRKYLSRRQAIIIQHLNRCMADIERIGDHIDELCDISVRRWSR
                     AEARFNRDTLEILCSLHEGVARVLRLVVQSLDPDNQDFQALAQAILRARDEYVERSIN
                     SKASFTEKLAAHEFSPIVGMFFSEYVGAYDRIVKHAKTIALAEKTPYFWIKRQKLERM
                     VDDEPDYKPPPLSDPHDFLDRLHSENYL"
     gene            2852..>4386
                     /locus_tag="PK634_13950"
     CDS             2852..>4386
                     /locus_tag="PK634_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOW98880.1"
                     /translation="MIAPRTEPPPESEFDLRAGVDAFFQPGGGLERACAGQPFPYETR
                     PQQREMALAVAEAIETSRHLAVEAGTGVGKSFAYLVPVLHAAVSRSIQVVVSTYTIAL
                     QEQLIRKDLPFLREHLGLPFKAVLVKGRSNYLCLRRLARARRMGGDLFASEGQDELEM
                     IRAWADRTADGSLQDMEQQPSADVWSSVCAEVGNCLGVKCREYRKCFFMKARAQVRNA
                     HLLVVNHHLLFSELALRTHGVSFLPDYRIVVLDEAHMTETVASEHLGIRLSHFAFEHW
                     MRRLYVPEKTRGLLAHLREGEAANGVVRVGEDADAFFAAVRQWAGLSEAKSQQVVSGP
                     MDVPGGLLESLQRMNGLLRNVIGKTQDEEIKSELRSAQMHGAEMRDMLEAFLKQSLPD
                     QVYWVEREGRRRQQTVLYSAPIEVGPLLAERLFEAMDCVIMTSATLAVCGKLDYFTQR
                     IGAAGCDALQLGSPFDYHRQMRVFIPRAMPDPNEADRYSGEAARAIAQYVRRTGGRAF
                     VLFT"
BASE COUNT          757 a         1426 c         1491 g          712 t
ORIGIN      
        1 gcatgccccg ctacggcgcg tccgccccgc agaaggtgct ggcctcggaa ttcggattca
       61 ccgcggagcg ggtggcggcg gcggcccgtg aaatgctggc gaagtagaaa cgaaacggaa
      121 ccccatgatc gacctccatt cccactccat cttttccgac ggctcgctga cgccggagga
      181 actcgtcgac caggcctgcg ccgcgggcct gactgccgtg gccctgacgg accacgacac
      241 cacctccggc ctgccgcgct tcctgaaggc cgccgggggc cggccgttgc gggcgatccc
      301 gggcgtggag atcagcgccg atttttcgcc gggcgccatg cacatgctgg gctatttcat
      361 ccggccggac gacgccgccc tcgaggagcg gctggtctgg atccgtgaag gccgcgacaa
      421 gcgcaacgcg gaaatcctgg agaagctcaa cgccctgggc ttcggcatcg cgtggaacga
      481 ggtggcgaag cacgccggcg cggacgtcgt gggccggccg catttcgccc aggtcctgct
      541 cgagcgcggc atcgtgaaaa ccaaggacga ggccttcgac ctctacctgg ggaagggcaa
      601 gcaggcctac gccgagcgcc gccgcttttc caccgaggat tccatcgcgc tgatccgcgc
      661 cgcgggcggc gtggccgtgc tggcgcatcc cttcacgctg ggcctggaca acggcaagct
      721 gcgcgagctg gtccagcggc tggccggcca ggggctgggc gggctcgagg tgtactactc
      781 cgagcattcc gccaatctcc agcaggccta catgaagctg gcccaggatt tcggcctggt
      841 cgccacgggc ggcagcgatt tccacggagc gctggcgccg gacatcaggc tgggcagcgg
      901 cttcggcggc ctgcgcgtgc cggacgaggt cgtggacaag ctcgaggcgc ggcgggatta
      961 caaatagttc tccgagtgga gccggtccag gaagtcgtgc ggatcggaca gcggcggcgg
     1021 cttgtagtcc ggttcgtcgt ccaccatgcg ctccagcttc tgccgcttga tccagaaata
     1081 gggcgtcttc tccgccaggg cgatggtctt ggcgtgtttc acgatccggt cgtaggcccc
     1141 gacgtactcg ctgaagaaca tgccgacgat gggggagaac tcgtgcgccg ccagtttctc
     1201 cgtgaacgag gccttcgagt tgatgctgcg ttccacgtat tcgtcgcggg cgcgcaggat
     1261 ggcctgggcc agcgcctgga aatcctggtt gtccgggtca agggattgca ccacgagccg
     1321 cagcacgcgc gccacgccct cgtgcaggct gcagaggatc tccagcgtgt cccggttgaa
     1381 acgggcctcg gcgcggctcc agcggcgcac ggagatgtcg cacagctcgt cgatgtggtc
     1441 gccgatgcgt tcgatgtccg ccatgcagcg gttgaggtgc tgaatgatga tcgcctgcct
     1501 ccgcgagagg tacttgcggg tgaggacgcc gaggtagtcc ttcatcgagc gcttgatctc
     1561 gtccacgacg ttctcgttca gcttgatcgc ctgaaccttc ttgcgctccg gccggaacag
     1621 gacgtcgatg gtgatggaaa agctctccgc gcagaccttc atcacgcgcc gcagctcgca
     1681 gatggccgcg tagatcgcct tctccgggaa ctggacgagc ttgcggtcga ggaaggttgg
     1741 cggcggcggc ggctttcgcg acggcatgag ccaggtaata aatcgcgcgt aggccgatgg
     1801 gatgggaagg accatcagcg cgcgcaccac catgacggcg gtgttcacat tggcggtctg
     1861 atgaataaga tccccggggg tccattcccc aaagcggata aaccaggggg cgaagacaat
     1921 ggccacaagg ctccccagca cattaaacat gagatgggac accgctgaac gacgggcctc
     1981 gatattggtg ccgatgctgc cgagcaaccc ggtggcacag gtgccgatgt tggcgccgag
     2041 gaccacggga tagacctggt tcaagtccgt cacggcgccg gcggaaatca gggcgaagca
     2101 catggcgatc acggccccac tgctctgaat gatgcccgtg accagcgtcg agatcagaac
     2161 gcccttcagc aggccgggca tcgtgtcgcc ctgaatgccc tggatgaagt ccgtaaacat
     2221 ttcccggtgg ggcttgatcg cgtcgctcat gatgttcagg cccaggaaga tgatgccgaa
     2281 cccgagtaac gactggccga cgtggcgaac cttgtcgttc gggatcacaa actggaggac
     2341 gaatcccgtt gtgatcagga aaaaacaata ggccccgagc ttgaaggaga ccagctgcat
     2401 ggagatcgtg gtgccgatat tcatgccgag catgacgccg acggattcca ccagggtcat
     2461 cagtccggcg ttgatgaagc ccacgatcat gaccgacgtg gcgctgctct ggatcagcgt
     2521 gccgagcacg agccccagga agaggccgcc gaaccggttg cgcgtcgcgc tggagagcag
     2581 ggtgcgcatg ctgctgccgg cggccttgcg cagggcgtcg gacatcatgt tcatgccctg
     2641 gatgaacagc acgaggcctc cgaggaccat cgcggtcagg aaaaagatat cccggcttcc
     2701 catcgccgcg accgccacat ccgccgcttg ccccgcgtcc atgatgcctg ctcctagctt
     2761 aaacaatgta aactgcgatg aatcgccgcc cttgtcactt aaaaaatagc gttccgtcgg
     2821 gggacatgct acatcccggg cggaccagcc catgatcgcg ccgcgcacag agccgccgcc
     2881 ggaatccgag ttcgaccttc gggccggcgt cgacgccttc ttccagccgg gcggcggcct
     2941 cgagcgggcc tgtgcgggcc agccgtttcc ctacgagacg cggccccagc agcgggaaat
     3001 ggcgctcgcg gtggcggagg ccatcgagac ctcccggcac ctggcggtcg aggccgggac
     3061 gggggtcgga aagagcttcg cctacctcgt cccggtcctc cacgccgccg tcagccggag
     3121 catccaggtc gtcgtttcca cgtacacgat cgcgctgcag gagcagttga tccgcaagga
     3181 cctcccgttc ctgcgggagc acctcgggct gccgttcaag gccgtgctgg tcaaggggcg
     3241 gagcaactac ctctgcctcc ggcgcctcgc ccgcgcgcgg cgcatgggcg gcgacctgtt
     3301 cgcgtcggag gggcaggacg aactggagat gatccgggcc tgggcggacc ggacggcgga
     3361 cggcagcctg caggacatgg agcagcagcc gtccgccgac gtctggagca gcgtgtgcgc
     3421 cgaggtcggc aactgcctcg gcgtgaaatg ccgcgaatac cgcaagtgct tcttcatgaa
     3481 ggcgcgcgcc caggtccgga acgcccacct gctggtcgtg aaccaccacc tgctgttctc
     3541 cgagctggcc ctgcggacgc acggcgtcag cttcctgccg gattaccgga tcgtggtgct
     3601 cgacgaggcg cacatgaccg agacggtggc gtccgagcat ctcgggatcc gcctgtcgca
     3661 cttcgccttc gagcactgga tgcggcgcct gtacgtgccc gagaagacca ggggcctgct
     3721 ggcccacctg cgcgagggcg aggccgccaa cggggtggtc cgggtgggcg aggacgcgga
     3781 cgccttcttc gccgcggtcc gccagtgggc ggggctctcg gaggccaagt cccagcaggt
     3841 ggtctccggc ccgatggacg tgcccggcgg cctcctggaa tcgctccagc ggatgaacgg
     3901 actcctgcgc aacgtgatcg ggaagacgca ggacgaggag atcaagtccg agctgcgctc
     3961 cgcccagatg cacggcgccg agatgcgcga catgctggag gccttcctca agcagtcgct
     4021 gccggaccag gtgtactggg tcgagcgcga gggccggcgc cggcagcaga cggtgctgta
     4081 ctccgcgccc atcgaggtcg gccccctcct ggccgagcgg ctcttcgagg cgatggactg
     4141 cgtgatcatg acgagcgcca ccctggccgt ctgcgggaag ctggactact tcacgcaacg
     4201 catcggcgcg gcggggtgcg acgcgctcca actcggctcg cccttcgact accaccgcca
     4261 gatgcgggtg ttcatcccgc gcgccatgcc cgacccgaac gaggccgacc gctacagcgg
     4321 ggaggcggcc cgcgcgatcg cgcagtatgt ccggcgcacc ggggggcgcg ccttcgtcct
     4381 gttcac
//
LOCUS       DAOOTK010000190         4345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_12728_length_4345_cov_4.979254, whole genome shotgun sequence.
ACCESSION   DAOOTK010000190 DAOOTK010000000
VERSION     DAOOTK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12728_length_4345_cov_4.979254"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK634_13955"
     CDS             <1..284
                     /locus_tag="PK634_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOW98881.1"
                     /translation="LQTGDDLSLTYQVEVTVQGDQLTLQGERKANELSANVACHRAER
                     GFGKFVRTFRLPFEVDNARATARCTNGVLTLTLPRTETSKPRRIAIAAE"
     gene            300..704
                     /locus_tag="PK634_13960"
     CDS             300..704
                     /locus_tag="PK634_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOW98882.1"
                     /translation="MTTPAKDMPTQKKEAAGAVELAQDRPVFLPPTDIYEREDAVLVV
                     CDMPGVDDQHVDVMLEDDVLTLTGSQEAQEPARHELLYRGHWPGVFRRSFTLTTEVDR
                     AKIKARIRHGVLEVVLPKAEAAQPKRIKVETD"
     gene            719..1222
                     /locus_tag="PK634_13965"
     CDS             719..1222
                     /locus_tag="PK634_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOW98883.1"
                     /translation="MKNLIPWRRKEPTALPRETANPFERLHREMNELFDGFLRDFGGT
                     ALPSLWRERAEDRVLPSVEVAETDESVQVTVELPGMDEKDIQVSLDDNALMVKGEKKQ
                     EREERKKNYRLSERSYGQFQRVIPLPAGIETDQVKAQFKNGVLNIILPKARSTAAESR
                     RIPVRSE"
     gene            1385..2656
                     /gene="eno"
                     /locus_tag="PK634_13970"
     CDS             1385..2656
                     /gene="eno"
                     /locus_tag="PK634_13970"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOW98884.1"
                     /translation="MTEIVEVLGREILDSRGNPTIEVEVELASGAQGRAAVPSGASTG
                     ENEAIELRDGDKARYLGKGVLKAVENVNNAIGTEIVGFDALDQAGLDRKLIDLDGTET
                     KSKLGANAILGVSLATAHAAAQHVGLPLYRYIGGTNAKVLPVPMMNVLNGGAHSDAPV
                     DVQEFMICPRGAPSFSEALRMGTEVFHALKGVLKKQGLSTAVGDEGGFAPALASNVAA
                     LDAIMKAIETAGYKAGKDVFICLDPAASEFYDTKKKKYVFKKSDKSEKTSEQMVDFWE
                     KWVKAYPIISIEDGMAEGDWEGWKIMTDRLGGKIQLVGDDLFVTNTKFLKKGIQKGVA
                     NSILIKVNQIGTLTETLDAIEMARQAKYTSVISHRSGETEDATIADIAVATNAGQIKT
                     GSASRTDRICKYNQLLRIEADLGNQAVYGLR"
     gene            2747..3061
                     /locus_tag="PK634_13975"
     CDS             2747..3061
                     /locus_tag="PK634_13975"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOW98885.1"
                     /translation="MSFWMGLYRAAWIALGVLFVIGAVAAFAPPIRQYRELRRMEADL
                     QEEIRLKEELLHHLKQQQERLQTDPEFVEKIAREELGLARPGETVYKFADDEPQTAPP
                     SP"
     gene            3058..3249
                     /locus_tag="PK634_13980"
     CDS             3058..3249
                     /locus_tag="PK634_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOW98886.1"
                     /translation="MKIDASKSFGERECPSCACRVPANHNRCPLCAYEFPALTARQRG
                     LKLGLALMMLAALLAWLLL"
     gene            complement(3256..>4345)
                     /locus_tag="PK634_13985"
     CDS             complement(3256..>4345)
                     /locus_tag="PK634_13985"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOW98887.1"
                     /translation="DFLASFVLTLAVFTFVMTLSAVVKAIDLLARGMSGVFILKVFLY
                     NIPFLLTFSIPISVLTTVLLLFGRLSFDGELTAMRSCGLTLWQILAPIVLASIGLTFL
                     CLYLNSSVAPRSHFARRQLLFSLGMEQPINLLEEGRFVRDFPGLMVYIGKKSGRRVED
                     VVVYEMADSGIKRNIRARSGEMEPGENRNVIIINLYDVRIDQPRAKGDDPSRSQYLTA
                     QHYPVRLDLNELAGSRVLHKKASDMTYPELLHAISHVQEVYRHLTPGELAKQRMSLVV
                     EASKRLALSLSCFAFVLLGAPLGMISRRKESSAGVGISLLLVFFFYFFIILANSLVGH
                     PEWRPDLIVWAPIVGAEIAGFWLVHRAH"
BASE COUNT          880 a         1372 c         1461 g          632 t
ORIGIN      
        1 acctgcagac gggcgacgat ttgagcctga cctaccaggt ggaagtgacc gtgcaggggg
       61 accagttgac gcttcagggc gaacgcaagg ccaacgagct gtccgcgaac gtggcctgcc
      121 atcgcgcgga gcgcggcttc ggcaagttcg tccgcacctt ccgcctgccg ttcgaggtgg
      181 acaacgcgcg ggcgacggcc cgttgcacga acggcgtgct gacgctgacc ctcccgcgaa
      241 cggaaaccag caagccgagg agaatcgcca tcgcggcgga ataacacagg agaacaccca
      301 tgactacacc ggccaaggat atgccgacgc agaagaaaga agcagcaggc gccgtcgaac
      361 tcgcgcagga ccggcccgtg tttctccccc cgacggacat ctacgagcgg gaagacgccg
      421 tcctggtcgt gtgcgacatg cccggcgtgg atgaccagca cgtggacgtg atgttggagg
      481 acgatgtcct gaccttgacg ggaagccagg aggcgcagga gccggcccgg catgagctgc
      541 tttatcgcgg ccactggccc ggcgtcttcc gccggtcgtt cacgctgacc accgaggtcg
      601 accgcgcgaa gatcaaggcg cgcatccgtc acggcgtgct ggaggtcgtg ctgcccaagg
      661 cggaggccgc ccagcccaag cggatcaagg tggaaacgga ctaggaagga ggcggaccat
      721 gaagaacctg attccctgga gaaggaagga gcccaccgcc ctcccgcggg aaacggcgaa
      781 tccgttcgag cggctgcatc gcgagatgaa cgagctgttc gacggcttcc tgcgggattt
      841 cggcgggacc gccctgccct cgctgtggcg cgagcgggcg gaggaccggg tattgccgag
      901 cgtcgaggtc gcggagaccg acgagtccgt ccaggtgacg gtcgaactgc ccgggatgga
      961 cgaaaaggac atccaggtga gtctggatga caatgccctc atggtgaaag gcgagaagaa
     1021 gcaggaacgg gaggaaagga aaaagaatta ccgcctctcg gagcgcagct acgggcagtt
     1081 ccagcgggtc atcccgctgc cggccggcat tgaaaccgac caggtgaagg cgcagttcaa
     1141 gaacggcgtg ctgaacatca tcctgcccaa agcccgctcg accgcggcgg agtcccgccg
     1201 cataccggtc cgttccgagt agcccgaccg cggcggcaga gccctttccc gggcgccgcc
     1261 atcgcgcctc gatcggcatg agtcacggtt cctgaacggt gactcatgcc gttgttcttg
     1321 catttcgccc gctttccccc tagctttgcc cggtctttta acgccaaccc atgggagaac
     1381 cgacatgacg gaaatcgtgg aagtgctggg cagggaaata ctggattcgc ggggcaaccc
     1441 gaccatcgag gtggaagtgg aactggcctc cggcgcgcag ggccgcgccg cggtgccgtc
     1501 gggcgcctcg accggcgaaa acgaggccat cgaattgcgg gacggcgaca aggcgcgcta
     1561 cctcggcaag ggcgtgctga aggccgtgga aaacgtcaac aacgcgatcg gcacggagat
     1621 cgtcggcttc gacgcgctgg accaggccgg cctggaccgc aagctcatcg acctggatgg
     1681 cacggaaacg aagagcaagc tgggcgccaa cgctatcctc ggcgtctcgc tggcgacggc
     1741 gcatgccgcg gcccaacacg tcggcctgcc gctctaccgc tatatcggcg gcacgaacgc
     1801 caaggtcctg ccggtgccga tgatgaacgt gctcaacggc ggcgcgcact cggacgcccc
     1861 cgtggacgtg caggagttca tgatctgccc ccggggcgcc ccctccttct ccgaggccct
     1921 gcgcatgggc acggaggttt tccacgcgct caaaggcgtg ctcaagaagc agggcttgag
     1981 cacggcggtc ggcgacgagg gcggtttcgc ccccgcgctg gccagcaacg tcgcggcgct
     2041 ggacgcgatc atgaaggcga tcgagacggc cggctacaag gccggcaagg acgttttcat
     2101 ctgcctcgat cccgccgcca gcgaattcta cgacaccaag aagaaaaagt acgtgttcaa
     2161 gaagagcgac aagtcggaaa agacttccga gcagatggtg gacttctggg aaaagtgggt
     2221 caaggcctac ccgatcatca gcatcgagga cggcatggcg gaaggcgact gggagggctg
     2281 gaagatcatg acggaccggc tgggcggcaa gatccagctc gtcggcgacg acctgttcgt
     2341 caccaacacc aagttcctca agaaaggcat ccagaagggc gtcgccaact cgatcctgat
     2401 caaggtcaac cagatcggca cgctgaccga gacgcttgac gcgatcgaga tggcgcggca
     2461 ggccaagtac acgtcggtga tcagccatcg ctccggcgag acagaggacg ccacgatcgc
     2521 ggacatcgcg gtcgcgacca acgccggcca gatcaagacc ggctcggcga gccgcaccga
     2581 ccgcatctgc aagtacaacc agctgctgcg gatcgaggcg gacctgggca accaggccgt
     2641 ctacggattg cgctgaacga aaccgccggg gccggcggac cgcccgccgg ccccgaaaaa
     2701 tacctgttga gaccgcgagg ggtggcggtc taccattgtt tccgcgatga gcttctggat
     2761 gggtctatac cgggcggcct ggatcgccct gggcgtgctg ttcgtcatcg gggcggtggc
     2821 cgctttcgcc ccgccgatcc gccagtaccg cgaactccgc cggatggagg ccgacctgca
     2881 ggaggaaata cgcctcaagg aggagctgct tcatcacctg aagcagcagc aggagcggct
     2941 gcagaccgat cccgaattcg tggaaaaaat cgcgcgcgag gaactgggcc tggcccggcc
     3001 gggcgaaacc gtctacaagt tcgcggacga cgagccgcag acggcgccgc cgtccccatg
     3061 aagatcgacg cctcgaagtc cttcggcgag cgggagtgcc cgtcctgcgc gtgccgggtg
     3121 cccgccaacc ataaccgctg ccccctctgc gcctacgagt tcccggccct gaccgcgcgg
     3181 caacgcgggc tgaagctggg cctggcgctc atgatgctgg cggccctgct ggcctggctg
     3241 ctgctttaat cggcgtcagt gggcccggtg gaccagccag aagcccgcga tctccgcgcc
     3301 gacgatgggc gcccagacaa tgagatccgg ccgccactcc ggatgaccga ccagcgagtt
     3361 ggccaggatg atgaagaagt aaaagaagaa caccagcagc aggctgatcc ccacgccggc
     3421 ggaggattcc ttgcgccgcg agatcatgcc cagcggcgcg cccagcagca cgaaggcaaa
     3481 acaggaaagt gacagcgcca gccgcttgct ggcctccacg acgagggaca tccgctgctt
     3541 ggccaactcc cccggcgtca ggtgccggta gacctcctgg acgtggctga tcgcgtgtag
     3601 cagctcgggg taggtcatgt cggacgcctt cttgtgcaga acgcgcgagc cggcgagctc
     3661 gttgaggtcc aggcgaacgg gatagtgctg ggccgtcagg tactgcgagc gactcgggtc
     3721 gtcgcctttc gcgcgcggct ggtcgatgcg cacgtcgtag aggttgatga tgatcacgtt
     3781 ccggttctcg cccggctcca tctccccgct gcgcgcgcgg atgttgcgct tgatgccgct
     3841 gtcggccatc tcgtagacca ccacgtcctc cacgcggcgg ccggatttct tcccgatgta
     3901 gaccatcagc cccgggaaat cgcggacgaa ccggccttcc tcgaggaggt tgatgggctg
     3961 ttccatgccg aggctgaaga gcagctgccg gcgcgcgaag tggctccgcg gggccaccga
     4021 gctgttcagg taaaggcaga ggaaggtcag gccgatcgag gcgaggacga tcggcgccag
     4081 gatctgccag agcgtcagcc cgcaggaccg catggccgtc aactcgccgt cgaaggagag
     4141 ccgcccgaac agcagcagca ccgtcgtcag gacgctgatg gggatcgaga aggtcaggag
     4201 aaacgggatg ttgtacagga agaccttcag gatgaagacc cccgacatcc cgcgcgcgag
     4261 caggtcgatg gccttgacca ccgcgctcaa ggtcatgacg aacgtgaaca ccgccagggt
     4321 caggacgaag gaggcgagga aatcg
//
LOCUS       DAOOTK010000191         4317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_12848_length_4317_cov_7.794697, whole genome shotgun sequence.
ACCESSION   DAOOTK010000191 DAOOTK010000000
VERSION     DAOOTK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12848_length_4317_cov_7.794697"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..257)
                     /locus_tag="PK634_13990"
     CDS             complement(21..257)
                     /locus_tag="PK634_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98888.1"
                     /translation="MNKLFSVLAALLASASALAGPLYCNILSGMESDVCYQHRPSRPW
                     VIEKQDGGMVYTTNILHWAQFGWREVPPVFWTGG"
     gene            complement(254..1138)
                     /locus_tag="PK634_13995"
     CDS             complement(254..1138)
                     /locus_tag="PK634_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98889.1"
                     /translation="MKLAISIVLILALSAMAQLPPWMAGVFVGEEAIEWTPESLGDTL
                     VMYADTDGAYCSSTNGSPPDNSDRVTQLVLRVGGVATNAIQCPTFVSGFTNGLGALRF
                     TQGETNVLYAATYSTYSNHTLFFVVSYTGAITSGTSAMYAETGNTGVGEGNAGYGALS
                     SAWSGETFAYYHYYSAVYGWCLKTNYPAGANVTCVQWLYPSTNISAFINGTVIPDSLK
                     ARDSGARGFTTNHYPTCFPRIGSIYNIPYEFHVLSFLAFDSVLDEPDRQKVEGYLAHR
                     YGFQDKLEASHPYKEGPP"
     gene            complement(1135..2787)
                     /locus_tag="PK634_14000"
     CDS             complement(1135..2787)
                     /locus_tag="PK634_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98890.1"
                     /translation="MLADFPSELQPLYETWLSENPDKAGQLASIISNTAAEIRALIAS
                     NPTNTLDEDETKIPLSLLRQAETLIYFTLSMEMGVSIAPEANQAMIRAEITLRTLVYG
                     RYLVSGGSSASQPSPLYNPEPERPTNRTLPVLAALLALFLGVTAAPAAWVKPGQTVSD
                     AAVYPTFAPSIYSNTTTTLFGHLQGIDAYLGSISHAYPSVFSSITVSRVQSPLFTGAG
                     TCNILICPYESASNPEWARSTVYLRGGLSGAEYGDTFIGTTNGSSDLYAYSWIYGRQI
                     FFVSPNSISFTSSSFSAKASSFSFDGPFALGMTNNGGFFSETFRAPGGSTSPKNLTLK
                     GADAYAVPNNGGAINIVGGDNAGGAYSRGSITIQGGSIGLSSSNGTTLSVSSNTLAIS
                     SDHSLFSAEARGPFAASSNAFVIRSQMDDALSGCLTNESDPVWAAASNLYARQADLAL
                     GIYTARLFAIDNDEFEIHPTNGGVQALSAAGDISLVAWPGTSTSDYEAVLLFINYEAS
                     LEIKATNGLVACGTIAITNGYWHGLYFEKPAGTTNKQYRKTR"
     gene            complement(2879..3820)
                     /locus_tag="PK634_14005"
     CDS             complement(2879..3820)
                     /locus_tag="PK634_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98891.1"
                     /translation="MSRLASISSDPVLKEFAQGVAQDSVMPVADFLAPTVPVSKSVGR
                     YKKYTEKNRFRIPDTLRALGGRATELKFEVSDDTYNCEPHALDYPVDNLEQLEADGIM
                     NMLREGAVAIAQVAALSHEKRVIDAALEAVGAGTSKTWDASSDPVSDVDTSILQVIKA
                     CAFGSVMNVGVLFGATAWKIFKNAAAVRNRFVVGSGGKAGVGLAVPSEQSANQLFIGT
                     PEVRTSYMVYDTQPEGKAADYSFLLDSTVLVFTRMAQPSRLDPSFMKTFRLMNNYMVP
                     SSYVRDDGRVEVAKFDWSEDVKVTNTAAAARLNISAS"
     gene            complement(3840..4253)
                     /locus_tag="PK634_14010"
     CDS             complement(3840..4253)
                     /locus_tag="PK634_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98892.1"
                     /translation="MNRILTLTAVLLVIVACFAFATDPESSFREGVRTQVMSNIYSVA
                     VSFPGNVTITSNATVAGNAAITGNQIVGGTTTAAVLKVLGAATVDGAATLTGGIAGGT
                     TVTQVWYSVPQSTASCVTNRLIIYKGVGQTLTQAP"
BASE COUNT          851 a         1206 c         1445 g          815 t
ORIGIN      
        1 gactctgcca aaatcttggc ttaaccacct gtccaaaaaa cggggggaac ttcacgccac
       61 ccaaactgcg cccagtgcag gatgttcgtc gtgtaaacca tgccgccgtc ctgcttctcg
      121 atgacccacg ggcgggatgg gcggtgctga tagcagacat ccgactccat gccggagagg
      181 atgttgcagt aaagcggccc cgcgagcgcg gaggcagatg cgagcaaagc cgcgagcacg
      241 ctgaacagct tgttcacggg ggaccttcct tgtaagggtg acttgcctcc agcttgtcct
      301 ggaacccgta ccggtgggcc aggtagcctt ccaccttctg ccggtccggt tcatcaagca
      361 cggagtcaaa ggccagaaac gaaagcacgt ggaactcgta cgggatgttg tagatggacc
      421 cgatgcgcgg gaaacaggtc gggtagtgat tggtggtgaa gccgcgggcc ccgctgtccc
      481 tggccttcag gctatcaggg attacagtgc cgttgataaa ggcggagata ttggtgctgg
      541 gatagagcca ctggacgcag gtcacgttgg cccccgccgg gtagttcgtc ttcaggcacc
      601 agccgtacac ggcggagtag tagtggtagt aggcgaaagt ctccccggac catgctgaag
      661 acaaggcccc gtacccggcg ttgccctctc ccacgccggt gttcccggtt tccgcgtaca
      721 tcgccgaggt gccggaagtg atcgcgccgg tgtaggagac gacgaagaac aacgtgtggt
      781 tgctgtacgt tgaatatgtg gcggcataca ggacgttcgt ctcgccctgc gtgaagcgta
      841 acgcgccgag gccgttcgtg aacccggaaa caaacgtcgg gcattgaatg gcattcgtcg
      901 ccacgccgcc gacgcgcaaa acaagctgcg tcaccctgtc gctgttatcg ggcggcgaac
      961 cattcgtgga tgagcaatag gccccgtcag tatccgcgta cataacgagc gtgtcgccga
     1021 gggattcggg agtccactcg atagcctcct cccccacgaa cactccggcc atccacggcg
     1081 ggagttgcgc catggccgag agcgcgagga tcaggactat gctgatggcg agcttcatct
     1141 ggtcttccga tattgcttgt tcgtggtgcc ggcaggcttt tcaaaataaa ggccgtgcca
     1201 gtagccgttt gtgatggcga tggtgccgca ggcgacaaga ccgttggtgg ccttgatttc
     1261 aagcgacgcc tcgtagttga tgaacagcag cacggcctcg taatccgacg tggacgttcc
     1321 gggccatgcg acgagagaaa tgtccccggc cgcggaaagc gcctgaaccc cgccgttggt
     1381 cgggtggatc tcgaactcgt cgttgtcgat ggcgaacagc cgcgctgtgt agatgcctaa
     1441 agcaaggtcg gcctgtcgtg cgtacaggtt gctcgccgcc gcccaaaccg gatcgctctc
     1501 gttcgtgagg cagccgctta acgcgtcatc catttgtgat cgtatgacaa aggcattgga
     1561 agatgcggcg aaggggccgc gagcttcggc ggagaacagg gaatggtcag atgaaatagc
     1621 cagggtgttt gacgagacag aaagggtcgt tccgttggag cttgaaagac ctatggagcc
     1681 gccttggatc gtgatcgaac cgcgagaata ggcgcctcca gcgttatccc cgccgacaat
     1741 gttgatcgcg cccccgttgt tcgggactgc ataagcgtcg gcgcctttca gcgtaaggtt
     1801 ttttggcgaa gtggaaccgc ccggggcgcg aaatgtttca gagaagaatc cgccgttatt
     1861 ggtcatgccc agggcaaacg ggccgtcgaa agaaaaagat gaagctttgg ctgaaaaaga
     1921 ggaagacgtg aaggaaatgg agtttggcga aacaaaaaat atctgccggc cgtaaatcca
     1981 ggaataggcg tacaggtcgg acgatccgtt ggtggttccg ataaaggtgt cgccgtattc
     2041 tgctcctgat aggccgccgc gcagataaac cgtggatcga gcccattcag gattggatgc
     2101 ggattcgtac gggcaaataa ggatgttgca cgtgcccgcg cctgtaaaaa gcggggattg
     2161 aacacgtgaa accgtgatgg aagaaaagac agagggatag gcgtgcgaga tggatcccag
     2221 ataggcgtct atgccttgca ggtgtccgaa tagagtggtg gtcgtattgg aatagatact
     2281 tggagcaaaa gtggggtaaa cggcggcatc ggaaactgtt tggcccggtt tcacccaagc
     2341 cgccggggcc gcggtgacgc ccaggaaaag ggccaacagc gccgccagga ccggcagcgt
     2401 gcggttggtg ggtcgttccg gctccgggtt gtagagcggc gagggctggc tggcgctgga
     2461 gccgccggat acgaggtagc ggccgtagac gagggtgcga agggtgatct cggcgcggat
     2521 catggcctgg ttggcctccg gcgcgatgga cacgcccatt tccatggaga gcgtgaagta
     2581 aatcagcgtc tcggcctggc gcagcagcga gagcgggatc ttggtctcgt cctcgtcgag
     2641 tgtattagtc gggttggacg cgatcagggc gcggatctcc gctgccgtgt tggagatgat
     2701 cgaggccagc tgcccggctt tgtcagggtt ctccgaaagc caggtctcgt agagcggctg
     2761 cagctctgac gggaagtccg ccagcatcag ctcttcagtt aaggcgaccc attccatatt
     2821 cagaacaaag ggccggagct ttgcggcccc ggcccccctc tccagttacc gaccccgatc
     2881 aggaggccga gatgttcagc cgcgccgctg cggccgtgtt ggtcaccttg acgtcctccg
     2941 accagtcgaa cttggcgacc tcgacacggc cgtcgtcccg gacatacgag ctcgggacca
     3001 tgtagttgtt catgagacgg aaggtcttca taaagctcgg gtccagccgg gacggctgcg
     3061 ccatccgggt gaacacgagg accgtcgagt ccagcaggaa gctgtagtcg gcggccttgc
     3121 cctcgggctg ggtgtcgtag accatgtacg aggtccggac ctcgggggtt ccgatgaaca
     3181 gttgattggc cgactgctcg gacgggaccg cgaggcccac gccggccttg ccgccagagc
     3241 ccacgacaaa gcggttacgg accgccgcag cgttcttgaa gatcttccag gcggtggcgc
     3301 caaagagcac gccgacgttc atgaccgagc cgaacgcgca agccttgata acctgcagga
     3361 tcgaggtgtc cacgtcagag acggggtcgg acgaggcgtc ccaggtcttg gaggtgcccg
     3421 cccctaccgc ctcgagggcg gcgtcaatga cgcgcttctc gtgggagagc gccgcgacct
     3481 gggcgatggc gaccgcgccc tcgcgcagca tgttcatgat cccatcggcc tcgagctgct
     3541 cgaggttgtc cacgggatag tccagggcgt ggggctcgca gttgtaggtg tcgtcggaca
     3601 cctcgaactt gagctccgtg gcccggccac ccagcgcgcg cagcgtgtcg gggatccgga
     3661 aacggttctt ctcggtgtac ttcttgtacc ggccgacgct cttggagacc ggcacggtcg
     3721 gggcgaggaa gtcggcgacg ggcatgacgg agtcctgggc gacgccctgg gcgaattcct
     3781 tcaggaccgg gtcgctgctg atggaggcga gtctgctcat tgggggtttc ctttcgtgct
     3841 tacggggcct gggtgagggt ttggccgacg cctttgtaga tgatgaggcg gttcgtgacg
     3901 caactggccg tggactgcgg cacggagtac cagacctgcg ttaccgtggt gccgccggcg
     3961 atcccgccgg tcagcgtggc cgcgccgtcc accgtggcgg ccccaagaac cttcaggacg
     4021 gccgcggtcg tggtcccgcc cacgatctgg ttgccggtga tcgcggcgtt gccggcaacg
     4081 gtggcgttgc tggtgatcgt cacgttaccg ggaaagctca cggccacgct gtagatgttc
     4141 gacatcacct gcgtgcggac gccctcgcgg aacgaggact cgggatccgt ggcgaacgcg
     4201 aagcacgcaa cgatcactag gagaactgcg gtcagagtaa gaatgcggtt catgttgggt
     4261 tccttgtgga ttagatgacg gtcacaaaac cgaggctcgc ggcccgcatg aggacca
//
LOCUS       DAOOTK010000192         4194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_13378_length_4194_cov_4.000242, whole genome shotgun sequence.
ACCESSION   DAOOTK010000192 DAOOTK010000000
VERSION     DAOOTK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13378_length_4194_cov_4.000242"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="PK634_14015"
     CDS             <1..1627
                     /locus_tag="PK634_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOW98893.1"
                     /translation="GQAVWNGRALYRGATAEESNLGRLQVGGRPSEHLGFVGGVTLKD
                     FGDLRGGSDVGKQEHTGYQEQDYDGRVDYYVNEDARFTLGHQTVRQDDAWRTHRTVYG
                     LDWEGLTPGDDKVHSYDQDRDLTYLRYGSENMAGFVDRMEVILSRHAQGEDLYRVRKD
                     DTSDTQGFDVVTWGAAVQLESDTDAGQWVYGAEYYRDLVDSYARKYKADGSLNKVEIQ
                     GPVADDATYDSVGVYVQDTLPLFDGRAELTPGARYSYAKADADRVKDPDTGDAMAIED
                     DWDTVVGSLRLLVPLAEDRRHVVFAGVAQGYRAPNLSDLTRFDIARSGELETPAPDLE
                     PERYLANEIGFKSRLEKLALQGSYYYTVIDDMIIRTPTGETIDDAAEVTKKNSGDGYV
                     QGLELSARYAFTAEWSAWAAGSLMDGRLDTYPSSDAEKDRDYVSRLMPPTAQVGLRWE
                     AEGGKWWSEALVDLADDADKLSADDKRDKQRIPPDGTPGYIVCTLRAGTRVADRLDLA
                     VALENLFDEDYRIHGSGVNEPGRSFVLTAALDL"
     gene            1694..2314
                     /locus_tag="PK634_14020"
     CDS             1694..2314
                     /locus_tag="PK634_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98894.1"
                     /translation="MNLAEFIGYAGSVIILISLLMRSLVKLRWINLAGSVLFSLYGLM
                     IRAWPVFGINAVIVGIDAWFLWQMSRLRDYFDLAPLSEIGADYFRKFFLYHENDIQAF
                     FPATPFESLARAETYLLFRNMLPVGFFSMELEGEEARVVADYVVPEYRDFKTGDFVYG
                     VKRMYFKEKGLKRFAAVSGNAVHQKYLLRNGFKPDATRPDLFVKML"
     gene            2418..3185
                     /locus_tag="PK634_14025"
     CDS             2418..3185
                     /locus_tag="PK634_14025"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOW98895.1"
                     /translation="MKTAILVIDIGNTSTTLGVYRGGRVIRMDRVPTADRAAAGLERR
                     LKRLAGSRAPEGVGVASVVPAAVGAWRKAARRLWPAAGWLEVGHRLELGVGIDYPRPA
                     TIGADRLCNACAAVERYGAPVIVVDFGTAVTFDVVSPERKYIGGIIAPGLPLMFSYLA
                     EKTALLPRIEWGRGVHPVGRSTVEAMRLGAIWGYRGLVREIKRELAAGFGWKNVTYCA
                     TGGYAARIMRGTGLAMRIDRDLTLFGIGRIYELNQVK"
     gene            3182..3985
                     /gene="trpA"
                     /locus_tag="PK634_14030"
     CDS             3182..3985
                     /gene="trpA"
                     /locus_tag="PK634_14030"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOW98896.1"
                     /translation="MKTRIDQCLERLRKTRRKAFIAYITAGDPGMAETVDSVVQLAGA
                     GADIVELGVPFSDPLADGRVNQDAATRALAAGATWGRLLKCIEQIRRKSPIPLILYSY
                     LNPLISRGFEKTARQATAAGADGFLIVDLPAEEMEPYSRILGRVGLNNICLVTPTSPS
                     ARIRKIAGLASGFVYCVSREGVTGMQKQLASSALGLIRRTRRWTRLPIALGFGISTPE
                     QARAAAREADVVVVGSAIVNRFAAAPKTAAGRKAAAQWTARLVRAVKEV"
     gene            3986..>4194
                     /locus_tag="PK634_14035"
     CDS             3986..>4194
                     /locus_tag="PK634_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOW98897.1"
                     /translation="MRDHYYAVILAGGRGERFWPLSTEKRPKQFIALAGDRTLLAQAV
                     ERLEGLIPPERILVITNKDLVGAAR"
BASE COUNT          721 a         1295 c         1471 g          707 t
ORIGIN      
        1 tggccaggcc gtctggaacg gccgggcgct gtaccgcggc gcgacggcgg aagaatcgaa
       61 ccttggccgg ttgcaggtcg gcggccggcc cagcgagcac ctgggctttg tcgggggcgt
      121 taccctcaag gatttcggcg acctgcgcgg cggctcggac gtggggaaac aggagcacac
      181 gggctaccag gaacaggatt atgacgggcg cgtggactac tacgttaacg aagatgcccg
      241 ctttaccctc ggccaccaga ccgtgcgcca ggacgatgcc tggcgcacgc accggaccgt
      301 gtacgggctc gactgggaag gcctgacccc cggcgacgac aaggtgcaca gctacgacca
      361 ggaccgcgac ctcacctacc tccggtacgg ctccgaaaac atggccggat tcgtggaccg
      421 gatggaagtc atcctctcgc gccacgccca gggggaggat ctctaccgtg tcaggaagga
      481 cgacacgagc gatacgcagg gcttcgacgt ggtcacctgg ggcgcggccg tccagctcga
      541 gtccgacacg gacgccggcc agtgggtgta cggggccgaa tactaccgcg acctcgtgga
      601 ttcctacgcg cggaagtaca aggcggacgg ctcgctcaac aaggtcgaga tccagggccc
      661 cgtggccgac gacgccacct acgattccgt cggcgtctac gtacaggaca ccctgccgct
      721 cttcgacggg cgggcggaac tgacgcccgg cgcgcgctat agctacgcga aggccgatgc
      781 cgaccgggtc aaggatccgg acaccggcga cgcgatggcc atcgaggacg actgggacac
      841 cgtggtcggc tcgctccgcc tgctggtgcc cctggcggag gaccgccgcc acgtggtctt
      901 cgcgggcgtg gcgcagggtt accgcgcgcc caatctctcc gatctcaccc ggttcgacat
      961 cgcgcggagc ggcgagttgg agacgcccgc gccggacctg gagcccgagc gctacctggc
     1021 caacgagatc ggcttcaagt cgcgcctcga aaaactggcg ctgcagggca gctactacta
     1081 caccgtgatc gacgacatga tcatccggac gccgaccggc gagaccatcg acgatgccgc
     1141 cgaggtcacg aagaagaact cgggcgacgg ctacgtccag ggcttggagc tgtcggcgcg
     1201 ttacgcgttc accgcggagt ggtccgcgtg ggcggcaggc tcgctgatgg acgggcggct
     1261 ggacacctat ccctcctccg acgccgagaa ggaccgcgac tacgtcagcc ggctcatgcc
     1321 gcccacggcc caggtcggct tgcgctggga ggcggaaggc ggaaagtggt ggagcgaggc
     1381 cctcgtggat ttggccgacg acgcggacaa gctgtccgcg gacgacaagc gcgacaagca
     1441 gcgcatcccg ccggacggca cgccgggcta catcgtgtgc acgttgcgcg ccggcacgcg
     1501 ggtggcggac cggctggacc tggccgtggc gctggagaat ctcttcgacg aggactaccg
     1561 gattcacggc tccggcgtga acgagcccgg gcgcagcttc gtcctgaccg cggcgctgga
     1621 tctctaaccg cgatacgggg gaggcgccgg tctccggcct tccctttacg cggggatgga
     1681 gtagtgtgag cctgtgaacc tggcggaatt cataggctac gccggctcgg tgatcatcct
     1741 gatctccctg ctgatgcggt cgctggtcaa gctgcgctgg atcaacctgg ccggctcggt
     1801 cctgttttcc ctgtacggac tgatgatccg cgcctggccg gtcttcggga tcaacgcggt
     1861 gatcgtgggc atcgacgcgt ggttcctctg gcagatgagc cggttgcggg attacttcga
     1921 tctggcgccg ctgtcggaga tcggggccga ttacttccgg aaattcttcc tgtatcacga
     1981 gaacgacatc caggcgtttt tcccggccac gccctttgaa tcgctggcgc gcgcggagac
     2041 ctacctgttg ttccggaaca tgctcccggt cggctttttc agcatggagc tggaaggcga
     2101 ggaggcccgg gtggtggccg attacgtcgt gccggaatac cgcgacttca agaccggcga
     2161 tttcgtgtac ggggtcaagc ggatgtactt caaggaaaaa ggcctcaagc gttttgccgc
     2221 cgtcagcggc aacgccgtcc accagaaata cctgctccgg aacggattca agcccgacgc
     2281 cacccggccg gacctgttcg taaaaatgct ctgatgcgga attttatctg taacaaaaac
     2341 attcacacgt gtgaatgctt ttgttatgcc catcttttgt atttcagccc ggggttgggt
     2401 ctggttaact ccgtccggtg aaaacggcga tccttgtcat agacatcggc aacacgagca
     2461 ccacgctggg cgtgtaccgg ggcggccggg tcatccggat ggatcgagtg ccaaccgccg
     2521 atcgggcggc ggccggcctg gaacgccgcc tgaagcgcct ggcgggatcc cgcgcgccgg
     2581 agggggtggg ggtggcttcc gttgtgccgg cggccgtggg ggcctggcgc aaggccgcca
     2641 gacggttgtg gccggcggcc gggtggctgg aggtcggtca tcggctggaa ctcggcgtgg
     2701 gcatcgatta cccgcgcccg gcgaccatcg gggcggatcg cctgtgcaac gcgtgcgccg
     2761 ccgtggagcg gtacggagcg ccggtcattg tcgtggattt cgggacggcg gtcaccttcg
     2821 acgtggtgtc ccccgagcgg aaatacatcg gcgggatcat cgcgccgggc ctgcctttga
     2881 tgttcagtta cctcgcggaa aagaccgccc tgctgccgcg cattgaatgg ggcaggggcg
     2941 ttcatccggt cggccgcagc acggtggagg ccatgcgcct cggcgccatc tggggctatc
     3001 gcggcctggt tcgagagatt aaacgggaat tggcggccgg attcgggtgg aagaatgtaa
     3061 cctattgtgc aacaggcgga tatgccgcgc gcatcatgcg cggaacaggg ctggccatga
     3121 ggattgacag ggatttgacc ttgtttggaa tcggccgcat ttatgaactg aaccaggtga
     3181 aatgaaaacg cgaatcgacc agtgtttgga acggttgagg aagacacgcc gcaaggcctt
     3241 cattgcctat atcacggcgg gcgatcccgg catggcggaa acggtggact cggtcgtcca
     3301 actggccggg gccggggcgg atatcgtgga actcggcgtg ccgttctccg atccgctggc
     3361 cgacgggcgg gtgaaccagg acgccgcgac gcgggcgctg gcggctggcg cgacctgggg
     3421 ccgccttctg aaatgcatcg agcaaatccg tcggaagagt ccaattcccc tgatcctgta
     3481 cagctacctg aatccgctca tttcgcgtgg cttcgaaaag acggcccgcc aggcgaccgc
     3541 ggccggagcg gacggcttcc tgatcgtgga cttgcccgcg gaggaaatgg agccgtactc
     3601 gcgcatcctc ggccgggtcg gcctgaacaa tatctgcctg gtgaccccga cgagcccgtc
     3661 ggcccgcatc cggaagatcg cggggttggc ctccggtttc gtctactgcg tctctcgcga
     3721 gggggtgacg gggatgcaga agcagttggc gtcctcggcc ctcgggctga tccggcggac
     3781 gcggcgctgg acgcgactgc ctatcgcgct gggattcggc atctcgacgc cggaacaggc
     3841 ccgggcggcc gcgcgcgagg cggacgtcgt ggtcgtgggc agcgcgatcg tgaaccgttt
     3901 cgccgccgcg ccgaaaacgg cggcgggccg gaaagcggcc gcccagtgga ccgcgcgcct
     3961 ggtccgggcc gtcaaggagg tttgaatgag ggatcattac tatgccgtca tcctggccgg
     4021 cggccgcggc gagcgcttct ggccgttgag caccgagaag aggcccaagc agttcatcgc
     4081 gctggccggg gaccggacgc tgctggccca ggccgtggaa cggctcgagg gattgatccc
     4141 gccggaacgc atcctggtga tcacgaacaa ggacctggtg ggcgcggcgc ggga
//
LOCUS       DAOOTK010000193         4125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_13706_length_4125_cov_4.443980, whole genome shotgun sequence.
ACCESSION   DAOOTK010000193 DAOOTK010000000
VERSION     DAOOTK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13706_length_4125_cov_4.443980"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK634_14040"
     CDS             complement(<1..716)
                     /locus_tag="PK634_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOW98898.1"
                     /translation="MKNEMVLAALLSISSMSLAETVTLQANEGEPGPVPVTLQNEARA
                     AIDRSAKWLLAQQQPEGNWSNPDFPALTALPLWALALTKFPEKEAMDKAFAYLLASVH
                     DDGSIWREPKAAQKGGGLSNYNTAICMTALHAVGDEKLVPVIQKARRFLAGSQHLGGD
                     IYEGGMGYDPETGRPYADLSNSYIAYEAMRLTENVEDLRQSGEEKADLDWEAAQKFIS
                     RVQNRADSNDQPWASDDPEN"
     gene            complement(783..3746)
                     /locus_tag="PK634_14045"
     CDS             complement(783..3746)
                     /locus_tag="PK634_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98899.1"
                     /translation="MRIKHVHCIPGRRSRSFWPCFPKAVLGAVACLLWIGLLAAPAQP
                     PGAPNRKLREYQELLQEAQRRGLDTGEAERLDRLSRAAAQGGDRAEAEQLLDQAIAML
                     EGALPADARPAEGAPPGRGPRPQGRPPWRERDPEPSGRGAVSAGGARQPVFLMAFTHH
                     YEGPGGYYPAAAEVRRMGEFFVRNGIPGTLFFDGILVERLQQEDPSILQQVREWGLPL
                     GYHGEETHGPYPVASELLGEVYTLREAQGYRGPWSLTTGKGWADAVALVEERYSHARP
                     YRIDESTRMLDRHQPSETDRARVGGLKLVQQVLGKDVSMLTSHGLESAPEGYAFRRMS
                     NFGLDQPAVPIALHALRIFRIGETADEIMKIAGGDESIFWCMGRLTCKGDEVGEAGYR
                     FGPLRHKLETLDRRRPRLLLMGFSKVEEQEGSQTARYLKEEFFPANPGSGWVTGDTLA
                     DHFELEKGYRPFAQDVSALARALVENWAGRPPDLVAVGERVYSLCDAFEALARSLAAD
                     QPGAPVELHALYGPVLEDDQPLLSRETSFATEAIRAAAASVVRDLDGATGDRFVPATV
                     SVGAVKLNSGEFMRAMAGALRSAGTQDTVPVAPSRAFPPYADLLQEVFKPRAVQPLCY
                     TKGQLWTVKPARLKNEAGAPSAAVPADGLKGKLTLVFAANLDADESCTRDSHGGADLY
                     RAEYDLATGAASNLQRLTSRAGLAEWFPALSPDGRVVVYDREEQPAPGRSRPSLHVLD
                     LATGADVLLADDARFASFSADGRALFFSRQERGEHSLWQAPFDTAGRSAQWGRPQLIA
                     DARTGGGILVEDPAPFPDGRGVAYHRKVDDMSGAQVAVIGADGSGPRALTPADGCGHA
                     AVSPDGQAVACTRSRDGRLVIIRKAGDTWSEPEEVALPDRPADYAAFDPRFGGVREVR
                     HSYAEWLAPDLLLLTTHGADGARDFRIARLFLVRLGAPGRTPALVDLSSAVEKMAGRA
                     GRDFCSAAGRLQQAD"
BASE COUNT          624 a         1469 c         1400 g          632 t
ORIGIN      
        1 ttgttctcgg gatcgtcgct cgcccacggc tggtcgttgc tgtccgcccg gttctggacg
       61 cgcgagatga acttctgcgc ggcctcccag tccaggtccg ccttttcctc gccggactgc
      121 cggagatcct cgacgttctc cgtcaggcgc atggcttcgt aggcaatgta gctgttggac
      181 aggtcggcgt acgggcggcc ggtctcggga tcgtagccca tgccgccctc gtagatgtcg
      241 ccgccgaggt gctgggagcc ggccaggaag cggcgcgcct tctggatcac gggcaccagc
      301 ttctcgtcgc cgaccgcgtg cagggcggtc atgcagatcg cggtgttgta gttgctcaag
      361 cccccgccct tctgcgccgc cttcggctcg cgccagatcg agccgtcgtc gtggacgctg
      421 gcgaggaggt acgcgaaggc cttgtccatg gcctccttct cggggaactt cgtcagcgcc
      481 agcgcccaca acggcagggc cgtcagggcg ggaaagtccg ggttggacca gttgccctcg
      541 ggctgctgct gggcgagcag ccacttcgcc gagcggtcga ttgccgcgcg cgcctcgttc
      601 tggagcgtca ccggcaccgg cccgggctcg ccctcgttcg cctggagggt cacggtctcg
      661 gccagagaca ttgaagagat cgacagcagc gcagccagga ccatctcgtt tttcatcgca
      721 tgcctcttta ataggaatag cgcagcagac gttctaatag ttcatcatcc ggccgcgttt
      781 cgtcaatcgg cttgttgcag ccggccggcc gccgagcaga aatcccgccc cgcgcggccc
      841 gccattttct cgacggccga ggagaggtcg acgagcgccg gcgtccggcc gggcgcgccc
      901 agccgcacaa ggaacagccg cgcgatccgg aaatcccggg ccccgtccgc cccgtgcgtg
      961 gtcagcagga gcaggtccgg cgcgagccat tccgcgtagc tgtggcgcac ctcgcgcacc
     1021 ccgccgaacc gcgggtcgaa cgcggcatag tccgcgggcc ggtccggcag cgccacttcc
     1081 tcgggctcgc tccacgtgtc cccggccttc cggatgatca ccaggcgccc gtcccgcgag
     1141 cgggtacagg ctacggcctg cccgtccggg ctgacggcgg cgtgcccgca accgtcggcc
     1201 ggggtcagcg cccgcgggcc ggacccgtcc gccccgataa cagccacctg ggcgccgctc
     1261 atgtcatcta ccttccggtg ataggcgacg ccccgcccgt ccgggaacgg agccgggtcc
     1321 tccaccagga tgcccccgcc cgtccgggcg tcggcgatga gctggggccg gccccattgc
     1381 gccgaacggc ccgccgtatc gaagggggcc tgccacaagc tgtgttcccc cctctcctgc
     1441 cgcgagaaaa agagggcgcg cccgtccgcc gagaaagacg cgaaccgggc gtcgtcggcc
     1501 aggagcacgt ccgcgccggt cgccaggtcg agcacgtgca gcgagggtcg ggaccgcccc
     1561 ggcgccggct gctcctcgcg atcgtagaca accacccggc cgtcgggcga caaggccggg
     1621 aaccactcgg caagccccgc ccggctcgtg aggcgctgga gattggacgc cgcgccggtc
     1681 gccaggtcgt actccgctcg gtacagatcg gccccgccat gcgaatcccg ggtgcaggat
     1741 tcgtcggcat ccagattcgc cgcgaagacc agcgtcagct ttcctttcaa gccgtcagcc
     1801 ggcacggcgg cggacggcgc gccggcctcg ttcttgagcc gggcaggctt gacggtccag
     1861 agttggccct tcgtgtagca cagcggctga accgcgcgcg gcttgaagac ctcctgcagc
     1921 agatcggcat agggcggaaa ggcgcgcgaa ggggcgaccg gcaccgtatc ctgcgtgccc
     1981 gccgatcgca gcgcgcccgc catggcgcgc atgaactcgc cgctgttcag cttcacggcg
     2041 cccacggaca cggtcgcggg tacgaatcga tcaccggttg ccccgtccaa gtcccggacg
     2101 accgaggccg ccgcggcgcg gatcgcttcc gtcgcaaacg aagtttccct ggacaacagc
     2161 ggttggtcat cctccagcac cggcccgtac agcgcgtgca gttccacggg cgcgccgggc
     2221 tgatccgccg ccaggctgcg cgccagcgcc tcgaacgcgt cgcacagcga atagacccgc
     2281 tcgccgacgg cgacgaggtc gggcggccgg cccgcccagt tctccaccag ggcccgggcc
     2341 agggcggaga cgtcctgcgc gaacggccgg tatcccttct cgagctcaaa atggtccgcc
     2401 agcgtgtcac cggtgaccca gccgctgccg ggattcgcgg ggaagaactc ctccttcagg
     2461 tagcgggcgg tctgcgagcc ctcctgctcc tcgaccttgc tgaagcccat cagcagcagg
     2521 cgcgggcggc ggcgatcaag cgtttccagc ttgtggcgga gcggcccgaa ccggtagccg
     2581 gcctcgccca cctcgtcgcc cttgcaggtc agccggccca tgcaccagaa aatactctcg
     2641 tccccgccgg cgatcttcat gatctcgtcg gccgtctcgc caatccggaa tatccgcagc
     2701 gcgtgcaggg cgatgggcac ggcgggctgg tcgaggccaa agttgctcat gcgccggaac
     2761 gcgtagcctt ccggggcgct ctcgagcccg tggctggtca gcatggacac gtccttgccc
     2821 aagacctgct ggaccagctt caggccgccg acgcgggcgc ggtccgtctc ggacggttga
     2881 tgccggtcca gcatgcgcgt ggactcgtcg atgcggtacg gccgcgcgtg cgagtagcgc
     2941 tcctcgacga gcgcgacggc gtccgcccaa cccttccccg tcgtgagcga ccacgggccg
     3001 cggtagccct gcgcctcgcg gagcgtgtac acttcgccga gcaattccga ggcgaccggg
     3061 tacggaccgt gcgtttcctc gccgtggtag ccgagcggca ggccccactc gcggacctgc
     3121 tgcaggatcg acgggtcttc ctgctgcagc cgctcgacca ggatgccgtc gaagaacagc
     3181 gtgcccggga tgccgttgcg cacgaagaac tcgcccattc tccggacctc ggcggcagcc
     3241 gggtaataac caccggggcc ctcgtagtgg tgcgtgaaag ccatcaggaa cacgggctgc
     3301 cgggctccgc cggcggagac ggcgccgcgg ccggagggct ccgggtcacg ctcgcgccag
     3361 ggcgggcggc cctgcgggcg cgggccgcgg ccgggcggcg ccccttccgc cgggcgcgcg
     3421 tcggcgggca gggcgccctc caacatcgcg atggcctgat cgagcaactg ctcggcctcc
     3481 gcgcggtcgc cgccctgcgc ggccgcgcgg gagagccggt cgagccgctc cgcctcgccg
     3541 gtgtccagcc cccggcgctg ggcttcctgg agcagttcct ggtactcgcg gagcttgcgg
     3601 ttgggggcgc cggggggttg ggccggggcc gcgaggagcc cgatccacaa caggcaggcc
     3661 accgccccca ggacagcctt cgggaagcag ggccaaaaag accgcgaccg acggccgggg
     3721 atgcaatgga cgtgttttat tctcataggc ccagcccgat aaccaactga ttactggaga
     3781 cttccgctga acgcccctat cacgagcgct tcacggggtt tcccttcatg gagcgcactg
     3841 gctcacaggg ctgaattcga gcccgaatcc ttctggttct cgcaggcggt ttatggaaca
     3901 gatcgcctct cgagacaatg aaaaatcatc gcgcccaggg aagcggcagg cggatcgcgc
     3961 gctttcgggg ctaccgacaa gcggggctcg gattgtattg acatgggccg gccggaatga
     4021 gatagtaatt attactagta cgcggcgctc gctggagcgc cggcggcaaa gtgacctggc
     4081 cccctgaagc gataccagcc ggatagtata tccggcaggc aaagc
//
LOCUS       DAOOTK010000194         4018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_14218_length_4018_cov_4.091850, whole genome shotgun sequence.
ACCESSION   DAOOTK010000194 DAOOTK010000000
VERSION     DAOOTK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14218_length_4018_cov_4.091850"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..144
                     /locus_tag="PK634_14050"
                     /pseudo
     CDS             1..144
                     /locus_tag="PK634_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(158..829)
                     /locus_tag="PK634_14055"
     CDS             complement(158..829)
                     /locus_tag="PK634_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOW98900.1"
                     /translation="MRIPIVKDGWWHMVGALIIPGAIAGFCLARGLNGLAMGFGALAV
                     VGSGFMLFFFRDPDRAIPEDPRALVAGADGLIRAIEVMPEDRFLKTEAVRVSIFLSPF
                     NVHINRTPIGGQVTALAYTPGRHLLTISNKASEFNEHSSILVEGQGTRCLVRQIVGPI
                     VRRVIYWLKPEQGLAKGDRIGLMKFGSRLDMYFPAADVEIRVKKGDKVRAGETVVAVI
                     TKDQR"
     gene            complement(1014..1964)
                     /locus_tag="PK634_14060"
     CDS             complement(1014..1964)
                     /locus_tag="PK634_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOW98901.1"
                     /translation="MIVTTKELFKHAYGKYAVGAYNINNLEQTMGLFQGNLDSKAPFI
                     VQLSKGARSYTHKKMVEALIRAADEIFPDAVFAVHLDHGDEATCMDCINSGFYSSVMI
                     DASGEPFDKNIEITRRVVDAAHAKGIVVEAELGKLGGVEEHVSVKEADAKLTDPEQAK
                     EFVRRSGCDSLACAIGTSHGAYKFAGSQGLHFEVLVEIQKRLPGFPLVMHGSSSVPQD
                     EVARINAAGGQLGGAKGVDENQFAKAATLGVTKVNIDTDGRLVWCRVHREAFRDDPKN
                     FDLRPPGKVFMKEYAKFIAHKNVKLGSAGQLDAVRALVKK"
     gene            2098..3951
                     /locus_tag="PK634_14065"
     CDS             2098..3951
                     /locus_tag="PK634_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOW98902.1"
                     /translation="MSAKPNAPSGIPALDEIVQGLRLGDNVVFQVDRLEDYLFFVRPF
                     ARRAVQDGRRCVYLRFAPHPPVLGPDDGFEIVPVDPGAGFDSFSGEVHRIIETRGHGV
                     FYVFDNLSALVAEWATDESLANFFQVTCPYLFELDTITYFALTRGHQAHATVARIRDT
                     TQLLLDVYHVQQRTYLHPLKVWDRYTPRMFLPHAMDGDAWTPVFQSGDAAAVVSVARQ
                     SPLRAPAESIAPWDSVFRRLLQYRESGMPLDESIPEIAALKQELTRMLIGDHPDFSRL
                     ADRHLTLDDLVAIRGRLIGSGRVGGKAAGMLVARRVLASVPDPEFTAALESHDSFYVG
                     SDVFFTFLVNNDLFRLRLKLSRSSHISREEFADVESRFLAGRFPADILEQFRTMLDYF
                     GQAPIIVRSSSLLEDSYGNAFAGKYRSEFCANQGSPEARLEAFLRAVKLVYASALNPD
                     ALTYRRRRGLGESEEQMAILVMRVSGMPHGSTFFPTLAGVAFSRNLYAWSDRIDARQG
                     VIRLVFGLGTHAVNRVGGDYPHMIAVSHPKLRPEIGLKIAKYSQHEMDLLDLQAKTLP
                     ELVLFQRTYRESEPSAKKNPGSVVEAMSRVMRTLSGRIIADVHAAIVAGKE"
BASE COUNT          647 a         1389 c         1254 g          728 t
ORIGIN      
        1 ggggatgttc gggatgccga cacgctccag gcgtgggtcc ggaaggtccg tccggagatc
       61 gtgttccacc tggccgccca acccctggtc cgccagtcct acgcccagcc tctggaaacc
      121 ctgtcggtga acgtgatggg aaccgccgga cgccagttca tcgttggtcc ttggttatga
      181 ccgccaccac ggtctcgccc gcccgaacct tgtcgccctt tttcacccgg atttccacgt
      241 cggccgcggg aaaatacatg tcgagccgcg acccgaattt catcagcccg atccggtcgc
      301 ctttcgccag gccctgctcc ggcttcagcc agtagatcac gcgccgcacg atcgggccga
      361 cgatctgccg caccaggcag cgcgttccct gcccttcgac caggatggag ctgtgctcgt
      421 tgaactcgga cgccttgttg ctgatggtca gcaggtgccg gccgggcgta taggccaagg
      481 ccgtgacctg cccgccgatc ggcgtccggt tgatatgcac gttgaacggg ctcaagaaga
      541 tgctgacgcg cacggcctcg gtcttcagga atcgatcctc gggcatcacc tcgatcgccc
      601 ggatcaggcc gtccgcgccc gccaccagcg ccctcggatc ctcggggatg gcccggtccg
      661 gatcgcggaa gaagaacagc atgaaccccg agcccacaac cgccagcgcg ccgaagccca
      721 tggccagacc attgagcccc ctggccagac agaacccggc tatcgccccg ggaatgatca
      781 gggcgccgac catgtgccac catccgtctt tgacgatcgg gatacgcatt accttatcca
      841 tcagcccgtt gcgcaagcct tgttgttggg gattgcgcag aggaagctat agacgatctc
      901 gtacgctgga aaaactatat aacggtttcc ccgcttgtac aaaacttttt cccggctgtt
      961 aaacaaaggg gcggcgatca agccgccccc ttgcttgccc acggatagct tttttacttc
     1021 ttgaccagcg cgcgcacggc gtcgagttgc cccgcgctgc cgagcttgac gttcttgtgc
     1081 gcgatgaact tcgcgtattc cttcatgaag accttcccgg gcggccggag gtcgaagttt
     1141 ttcgggtcgt cgcggaacgc ctcgcggtgc acgcggcacc agaccagccg gccgtccgtg
     1201 tcgatgttga ccttggtcac gcccagcgtc gccgccttgg cgaactggtt ttcgtccacg
     1261 cccttcgccc cgccgagctg cccgccggcg gcgttgatgc gcgcgacctc gtcctgcggc
     1321 acggagctcg acccgtgcat gaccagcggg aagccgggca ggcgcttctg gatttccacc
     1381 agcacctcga aatgaaggcc ctggctgccc gcgaacttgt aggcgccgtg gctggtgccg
     1441 atggcgcacg ccaggctgtc gcagccgctg cgccgcacga attccttcgc ctgctccggg
     1501 tccgtcagct tggcgtcggc ctccttgacg gagacgtgct cctccacgcc gcccagcttg
     1561 ccgagctcgg cctcgaccac gatccccttg gcgtgcgcgg cgtccacgac gcggcgcgtg
     1621 atctcgatgt tcttgtcgaa cggctcaccg ctggcgtcga tcatgacgga gctgtagaag
     1681 ccgctgttga tgcagtccat gcaggtggcc tcgtccccgt ggtccaagtg gacggcgaag
     1741 acggcgtccg ggaagatctc gtcagccgcc cggatgagcg cctcgaccat cttcttgtgc
     1801 gtgtaggacc gcgcgccctt ggacaactgg acgatgaacg gcgccttgct gtccaggttg
     1861 ccctggaaca ggcccatggt ctgctcgagg ttgttgatgt tgtacgcccc gacggcgtat
     1921 ttcccgtacg cgtgcttgaa cagttctttc gttgtaacga tcatggagtg ctcccttggg
     1981 ttgatcccgc aaagcgcgat acaccatacg cgatggttcc cggttgtaaa gggtaaaacg
     2041 caataccggg acgcaaggcg gcttgatgcc ggaagtcgcc ccgggcatag tgccgacatg
     2101 tccgccaaac ccaatgcccc gagcggcatc ccggcgctgg acgaaatcgt ccaggggctg
     2161 cgcttgggcg acaacgtggt gttccaggtc gaccggctgg aggattacct gttcttcgtc
     2221 cgccccttcg cccggcgcgc ggtccaggac gggcggcgtt gcgtctacct ccgcttcgcc
     2281 ccccacccgc cggtgctcgg gcccgacgac ggcttcgaga tcgtgccggt ggacccgggc
     2341 gccgggttcg actcgttcag cggggaggtg caccgcatca tcgagacccg cgggcacggg
     2401 gtgttctacg tcttcgacaa cctctccgcg ctcgtcgctg agtgggccac ggacgagtcg
     2461 ctggcgaatt tcttccaggt cacctgcccg tacctcttcg agctggacac gatcacctac
     2521 ttcgccctga cccgcggcca ccaggcccac gccaccgtgg cgcgcatccg cgacacgacc
     2581 cagctgctgc tcgacgtcta ccacgtccag cagcgcacct atctccaccc gctcaaggtc
     2641 tgggaccgct acacgccgcg gatgttcctg ccgcacgcga tggacggcga cgcgtggacg
     2701 ccggtcttcc agagcggcga cgccgctgcc gtcgtgtccg tcgcgcgcca gtccccgctc
     2761 cgcgcgccgg ccgaatccat cgcgccgtgg gacagcgtct tccgccggct ccttcagtac
     2821 cgcgaaagcg gcatgccgct ggatgaatcg atcccggaga tcgcggccct gaagcaggag
     2881 ctgacgcgta tgctgatcgg ggaccacccg gacttcagcc ggctggcgga ccggcacctg
     2941 acgctggacg acctggtggc gatccggggc cggctgatcg ggtcgggccg ggtcggcggc
     3001 aaggccgccg gcatgctcgt cgcccggcgc gtgctggcct ccgttccgga tccggaattc
     3061 accgccgccc tggagagcca tgactcgttt tacgtcggct cggacgtgtt cttcaccttc
     3121 ctggtcaaca acgacctctt ccgcctgcgc ctcaagctgt cccgcagctc gcacatctcg
     3181 cgcgaggagt ttgccgacgt ggagagccgg ttcctcgcgg gccgcttccc ggcggacatc
     3241 ctggagcagt tccggacgat gctggattac ttcggccagg cgccgatcat cgtgcggtcc
     3301 agcagcctgc tggaggacag ctacggcaac gcgttcgcgg gcaagtatcg cagcgagttc
     3361 tgcgccaacc agggcagccc ggaggcgcgg ttggaagcct tcctgcgcgc ggtcaagctg
     3421 gtgtacgcca gcgcgctcaa cccggacgcg ctgacctacc gccggcggcg cgggctcggg
     3481 gagagcgagg agcagatggc catcctcgtc atgcgcgtgt ccggcatgcc gcacggctcg
     3541 accttcttcc ccaccctggc cggcgtcgcc ttctcgcgca atctctacgc gtggagcgac
     3601 cgcatcgacg ccaggcaggg cgtcatccgg ctggtcttcg ggctcggcac gcacgcggtc
     3661 aaccgggtgg gcggcgacta cccgcacatg atcgcggtca gccacccgaa gctccgcccc
     3721 gagatcggcc tcaagatcgc caagtactcg cagcacgaga tggacctgct cgacctgcag
     3781 gccaagacgc tgcccgaact ggttctcttc cagcgcacct accgcgagtc ggagcccagc
     3841 gccaaaaaaa atcccggctc ggtggtcgaa gccatgagcc gggtcatgcg cacgctgtcg
     3901 gggcggatca tcgccgacgt gcatgccgcg attgtcgctg ggaaagagtg aatggtgaac
     3961 ggtgagtggt gaatggcaaa atcaaagaac agttcgcagc catccgcgtg gctccact
//
LOCUS       DAOOTK010000195         3905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_14858_length_3905_cov_3.052727, whole genome shotgun sequence.
ACCESSION   DAOOTK010000195 DAOOTK010000000
VERSION     DAOOTK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14858_length_3905_cov_3.052727"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /gene="aceE"
                     /locus_tag="PK634_14070"
     CDS             complement(<1..1838)
                     /gene="aceE"
                     /locus_tag="PK634_14070"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOW98903.1"
                     /translation="MSESKQSDPAALDLETRDWIDSFNDLLERTGPEGAQRILREVQV
                     HAQRSGVQMPFSANTPYINTIPVGDQPPYPGNREIERRIKSIIRWNAMAMVVRANREE
                     AGIGGHISTYASAATLYEVGFNHFFRGRSDGHPGDLVYFQGHASPGIYARAFLEGRLP
                     LERIDNFRHELHEKPGLSSYPHPWLMPDFWQFPTVSMGLGPITAIYHARFIRYLEDRG
                     LKERSDQKIWAFLGDGETDEPETLGAITLASREKLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELEAAFRGAGWNVIKVVWGGDWDPLLEKDRDGLLVRRMGEAVDGDYQKYVVSSGD
                     YIRQHFFGQYPELLEMAAHLSDEQLQKMRRGGHDPEKVFAAYQAAVTNRGTPTVILAK
                     TIKGYGLGESGEGKNITHQQKKLNEDELREFRSRFGIPISDAEIDKVPFYRPPDDSPE
                     MEYLQERRKALGGYLPERRPTTVSVGAPAPDTFQEFLAGTGDREVSTTMAFVRILAKL
                     LKDPKLGKHIVPIVPDEARTFGMEALFRQCGIYSHAGQLYEPVDADSLLYYKEAQNGQ
                     ILEEGINEAGSMSSFIAAGTAYANHGISMIPFFIYYSMFGFQRVGDL"
     gene            complement(1777..3211)
                     /locus_tag="PK634_14075"
                     /pseudo
     CDS             complement(1777..3211)
                     /locus_tag="PK634_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(3208..3516)
                     /locus_tag="PK634_14080"
     CDS             complement(3208..3516)
                     /locus_tag="PK634_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98904.1"
                     /translation="MQRVLDFLNRNRLRATYGDVARFLGIPMPALAELLGPPRPAASW
                     VVNSETGKPSGYAEADCHPDLRSHDVIVDEADLRLKIQREKTNRPSIRAARRPHRRIA
                     "
BASE COUNT          597 a         1250 c         1376 g          682 t
ORIGIN      
        1 aggtcgccta cccgctggaa cccgaacatc gagtagtaga tgaaaaacgg gatcatgctg
       61 atcccgtggt tcgcatacgc cgtgcccgcc gcgatgaacg acgacatgga gccggcctcg
      121 ttgatgcctt cctccaggat ctggccgttc tgcgcctcct tgtaatacag gaggctgtcg
      181 gcgtcgacgg gctcgtacaa ctggccggcg tgggagtaga tgccgcactg gcggaagagc
      241 gcctccatgc cgaacgtgcg cgcctcgtcg gggacaatgg gcacgatgtg cttccctaac
      301 ttcggatcct tcagcagttt ggccaggatc cggacgaacg ccatggtcgt ggacacctcg
      361 cggtcgcccg tgccggcgag gaattcctgg aacgtgtccg gtgccggcgc gccgacggaa
      421 accgtcgtcg gccggcgctc gggcagatag ccgcccaggg ccttccgccg ctcctgcagg
      481 tattccatct ccgggctgtc gtccggcggc cggtagaagg gcaccttgtc gatctccgcg
      541 tcggagatcg ggatgccgaa ccgggagcgg aactcccgca gttcgtcctc gttgagcttc
      601 ttctgctggt gcgtgatgtt cttgccctcg ccgctctcgc cgagcccgta gcccttgatg
      661 gtcttggcca ggatgaccgt cggcgtgccg cggttcgtca cggcggcctg gtaggcggcg
      721 aagaccttct cgggatcgtg cccgccgcgc cgcattttct gcagttgctc gtccgagagg
      781 tgggccgcca tctcgaggag ctccgggtac tggccgaaga aatgctgccg gatgtagtcg
      841 ccgctggaga cgacgtactt ctggtaatcg ccgtccaccg cctcgcccat gcgccgcacg
      901 agcaggccgt cgcgatcctt ctccagcagc gggtcccagt cgccgcccca gacgaccttg
      961 atcacgttcc agccggcgcc gcggaacgcc gcctcgagtt cctggatgat cttgccgttg
     1021 ccgcgcacgg ggccgtcgag gcgttgaaga ttgcagttga tcacgaagat caggttgtcg
     1081 agtttctcgc gggaggcgag ggtgatcgcg ccgagcgtct cgggctcgtc ggtctcgccg
     1141 tcgccgagga acgcccagat cttctgatcg ctcctctctt tgagcccgcg gtcctccagg
     1201 tagcggatga agcgcgcgtg gtagatggcc gtgatcgggc ccaggcccat cgagaccgtc
     1261 gggaactgcc agaagtccgg catcagccag ggatgcgggt acgacgacag gccgggtttc
     1321 tcatgcaact cgtgccggaa attatcgatt cgctccagcg gcaggcggcc ctccaggaac
     1381 gcccgcgcgt agatgccggg cgaggcgtgg ccctggaagt agaccaggtc gccgggatgg
     1441 ccgtcgctcc ggccgcggaa gaaatggttg aagcccacct cgtagagcgt ggcggctgag
     1501 gcgtaggtgg agatgtggcc gccgatgccg gcctcctcgc ggttggcgcg aacgaccatg
     1561 gccatggcgt tccaccggat gatgctcttg atgcgccgct cgatctcgcg gttcccgggg
     1621 tagggcggct gatcgccaac cgggatggta ttgatgtacg gcgtgttggc cgaaaagggc
     1681 atctgcacgc ccgagcgctg cgcgtggact tgcacctcgc gcaggatcct ctgcgcgccc
     1741 tccgggcccg tgcgctccag caggtcgttg aaggaatcaa tccagtcgcg ggtctctagg
     1801 tccagggccg ccgggtccga ttgcttcgat tcgctcatag tccattcctt gccatgcggg
     1861 agaaggcgcg cggggcgcca cgatgaccgc taacataacc gataaatcag cggcttcaac
     1921 tggaaatccc ggcccctgcg gacggaaact agcggtggaa acgtttccgg cgccggcgcg
     1981 gcccgaacga gcgggtgccg ccccggggcg gcgcggccga cggcctcggg cccgcctgcc
     2041 cggcgggcgc cggaaccgag gctcggtgat ggggcgaggg ctgggcccgc gccgcgtagt
     2101 cgaatccgtc gagcgtccgg cgctcgatct tcgccccgag cacgcgctcg atgccccgga
     2161 ccacgccctc gtcttccggc gtgacgaagg tgaaggcgtc cccggtcttg gccgcgcgcc
     2221 cggtgcggcc gatgcgatgg gtataatcat ccaccgtctt gggcatgtcg tagttgatga
     2281 catgcgagat gctggcgacg tcgatgccgc gggccgccag gtcggtggcc acgagcacct
     2341 ggaaccggcc gctccggaac ccgtccatgg ccacctgccg ctgccgctgg gacatattcc
     2401 cctgcaggca ggcggctcgg tgcccggtct tgtccaggtg cgcggcgagc cgcttggcgc
     2461 ggtgcttggt gcgcgtgaag atcaggacgg actggcggtc ggtgcgctcg aggagcgccg
     2521 tcagcagggg cgttttaaga tgcgtggcca ccgggaacag ggcgtgggaa acggattccg
     2581 ccggcttgga atgcgcgatc tgcatggtga cgggattgtg cagaatctcg tgcgccagtc
     2641 cgcggatggc gtcgggcatc gtggccgaga agagcagggt ctggcgccgg gccggcagcc
     2701 tcgccaggat tttccggatg tccggcagga agcccatgtc gaacatgcgg tcggcctcgt
     2761 cgagcaccag gccttccacg tgcgacagcg tgatggtgtt ctgccggatg tggtccagga
     2821 gccggccggg gcaggcgacg acgatctccg ccccgtgccg gagcccggcg acctgcggat
     2881 tcatgccgac gccgccgtaa atggtcacgc agtgaagatg ggtgtgcgcg ccgaggcggt
     2941 ccagcgcctc gcgggtttgc tccgccaatt cgcgggtggg gccgatgatc aggaaccgga
     3001 gctggcggcg cgggccctgc atcaagcggt gaagcagggg gatgccgaag gccgcggtct
     3061 ttccggtgcc cgtctgggcg aggccgatga cgtcgcgtcc ctccaggatc gcggggatcg
     3121 tcttgcgctg gatgggtgtc ggcacatcga agccgagcgc cctgatattt tcccggatgc
     3181 gcgcgtcgag ggagagtccg tcgaaactca tgcgatcctc cggtgcggcc ggcgcgcggc
     3241 gcggatggac ggcctgttcg tcttttcacg ctgaatcttc aggcgcaggt cggcctcgtc
     3301 gacgatgaca tcatgggacc gcaggtccgg atggcaatcg gcttccgcgt agcccgaggg
     3361 ctttccggtt tcgctgttga cgacccacga ggccgccggc cggggcgggc cgagaagttc
     3421 ggccagggcc ggcatgggaa tccccaggaa ccgggcgaca tccccatacg tcgcccgcag
     3481 cctgttccgg ttcaggaagt ccagaacccg ttgcacgttg cactccatgg gccactccct
     3541 tctgtgtgat cccgggcagg ccttggcccg gccgtcgggg ggaactttca ggccgggttc
     3601 catcgcgaac cggtgagaga gctctcaaca ggcaaaatac tcgttcgggc gcctgatggc
     3661 aatgcccgtt ccggggagcc ggcaggattc ccgaggccgg gcgaaatcat ccgaaagcga
     3721 gccccgcctg tgtcggccca tgcaggggcg ctggatttca gcaggggccg tgggcgccgg
     3781 acgccgcatc gaagggcaca cgcgcggcga cgcgtcccct gaaccgaagc gaatccgtgt
     3841 aaccgctttc gcgggccagg cggacggctt gctcaaacgc gctgcccacc tggtccggtt
     3901 ggtgg
//
LOCUS       DAOOTK010000196         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_14929_length_3890_cov_3.811473, whole genome shotgun sequence.
ACCESSION   DAOOTK010000196 DAOOTK010000000
VERSION     DAOOTK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14929_length_3890_cov_3.811473"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..2977)
                     /locus_tag="PK634_14085"
     CDS             complement(26..2977)
                     /locus_tag="PK634_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW98905.1"
                     /translation="MIAEPVLNGLLHLFALAAAPLRGEAREAARRRTLDYLNNHVGLA
                     DAGVYIGLYQDLAELHAIGDEADRLRAAGEIVASLKTQLGGEERYAAVLCVLELAAVS
                     AGSDWPGRMAEWLGRELDLAEEAGRMRAFLTCPPGADGRWMGGGTAGFRGRLAVLKLR
                     GDDLFLAAATGDEPVWLEGHPLAPGAARLLCPGQVLRDRWGNELHYAAVAAAFREEGA
                     GPVLDFRGARLEYRFADGRCGLHDFSFAERGGRLVGIMGASGAGKSTLLEILNGTLRP
                     SSGDVRLNGRDIHAGAGEGVIGFVPQDDMLFEDLTVYENLYYAARLCLAHLPEARRIA
                     RVRRMLADLGQADIADLKVGSPLQKTISGGQRKRLNIALELIREPTVLFVDEPTSGLS
                     SADSETVMSLLKEQAAGGRLVFAVVHQPSSKIFRMFDALWILDQGGWPVFTGAPVEAV
                     AYFRAHGNLPGAWESICPGCGSVNPEQIFDILEARTLGPAGRATPRRRVPPEQWHERY
                     RDYEAERARPAAPVPPAELPPPAPSLHRPGRLGQLAVFFVRDVRARLASLSYLLVALL
                     EPPVLGLLTGLVSRGAFGGTYSFHENNNVHVFFFMSVIVAVFLGLSVSAEEICRDARI
                     LRRERFLHLSWWSYVHSKTLYLALVCALQMALFVSVAFPIVGVPGLLVRGWAALFLCA
                     FCSGLLGLNISASFRSAVTIYILIPLLIVPQMLLCGVVIPYDDLIAPRSRAREVPWFA
                     NLMPPRWGYEALVVEQYLHNAYQRNLAGADARLRLAEFDLDHYLPELLGLVQSIPLLR
                     EEGRAGDLAAKQRILTNELARLEQRMGTAAGAAPAVSGPDLLDDAGRARVEEYLAACR
                     KTVFEQRRAAAADKRAILDRLEAELGPEGLERLKRRHTSRSIEKQALNLQDFRPVGEA
                     EDGLFRRTLPVYQVPESCWGGAHFLAGSKRWGGRLRATYAFNLAAIAALSFGLYGLLG
                     LRVLPRLLRNR"
     gene            complement(2992..>3890)
                     /locus_tag="PK634_14090"
     CDS             complement(2992..>3890)
                     /locus_tag="PK634_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98906.1"
                     /translation="LLVDVALALGGTAAVAEAPPAPSEAAPQAAVEALKQLSAEVQEI
                     LVRENVHSMFDRTARLQELGLSYQPDLVAPAAIDPAWTRCQQQQFAGIKLFDAVYAIA
                     FQKRQETADAMRALEEIITGLDLRSHADFSGRSYATLQKAAEDPGAIDVQALLDELAN
                     NLADEIPVTMSSPVTAHFLMDSLYGFSIESAHVMGYFQRNDATGKLRAQLYEEPSQPG
                     REPWMKMVLRAFAAFERQAAALGLECDAPGKLDVVRSIAQVVEDEQAGRIPPETGAAI
                     WNEIGEQIAAMRRSILRAGAGN"
BASE COUNT          593 a         1377 c         1396 g          524 t
ORIGIN      
        1 gggcgcgggc cggcgccggc ccgcgtcagc ggttccgcag cagccggggc aggacccgga
       61 gccccagcag gccgtacagg ccgaaggaca gcgccgcgat cgccgcgaga ttgaaggcat
      121 aggtagcccg cagccggccg ccccaccgct tgctccccgc caggaaatgc gcgccgcccc
      181 agcacgactc cggaacctgg tagacgggca gcgtcctccg gaacagcccg tcctccgcct
      241 cgccgacggg ccggaagtcc tgcaggttga gggcctgctt ctcgatggac cggctcgtat
      301 gccgccgctt cagccgctcc aggccttccg gcccgagttc cgcctcgagc cggtccagga
      361 tcgcgcgctt gtccgcggcg gccgcccggc gctgttcgaa gacggtcttg cggcaggccg
      421 ccaggtattc ctccacccgc gcgcggcccg cgtcgtcgag caggtccggc ccgctcacgg
      481 ccggcgccgc gccggccgcc gttcccatgc gctgttccag ccgggccagc tcgttcgtca
      541 ggatccgctg tttcgcggcc aggtcgccgg cgcgcccctc ctcccgcagc agggggatcg
      601 actggaccag gcccagcagc tccgggaggt agtggtccag gtcgaactcg gcgaggcgca
      661 gccgcgcgtc cgccccggcc aggttccgct ggtaggcgtt gtgcaggtac tgctccacga
      721 cgagcgcctc gtagccccag cgcggcggca tgaggttggc gaaccagggc acctcccgcg
      781 cgcgcgaccg cggcgcgatg agatcgtcgt acgggatcac cacgccgcac agcagcatct
      841 gcgggacgat cagcagcggg atgaggatgt agatcgtcac ggccgagcgg aaggacgcgg
      901 agatgttcag gcccagcagc ccggagcaga acgcgcacag gaagagcgcg gcccagcccc
      961 ggaccagcag gcccggaacc ccgacgatcg ggaaggcgac ggacacgaag agcgccatct
     1021 ggagggcgca gacgagggcg aggtagaggg tcttggaatg gacgtagctc caccagctca
     1081 agtggaggaa ccgctcgcgc cgcaggatgc gcgcgtcgcg gcagatctcc tcggcgctga
     1141 cgctcaaacc caggaacacc gcgacgatga cactcatgaa aaagaagacg tgaacgttgt
     1201 tgttctcgtg aaacgagtag gttccgccga acgcgccgcg cgagaccagc ccggtcagca
     1261 ggcccaggac cggcggctcc aggagcgcga ccagcaggta ggacaggctg gccaggcgcg
     1321 cccggacgtc gcgcacgaaa aagacggcca actgaccgag ccggcccggc cggtgcaggc
     1381 tcggggcagg cggcggaagt tccgccggcg ggacgggcgc cgccggccgc gcgcgctccg
     1441 cctcgtagtc gcggtaccgc tcgtgccact gctcgggcgg aacccgtcgc cgcggcgtgg
     1501 cccggccggc cgggccgagg gtgcgggcct cgaggatgtc gaagatctgc tcgggattca
     1561 cgctgccgca gcccgggcag atggactccc aggcccccgg caggttgccg tgcgcgcgga
     1621 agtacgcgac ggcctcgacg ggggcgcccg tgaagaccgg ccaaccgccc tggtcgagga
     1681 tccacagggc gtcgaacatc cggaatatct tcgaggacgg ctggtggacc accgcgaaga
     1741 caagccggcc gccggcggcc tgctccttga gcaggctcat gacggtctcc gagtcggccg
     1801 aggaaaggcc ggacgtgggc tcgtccacga acagcaccgt cggctcgcgg atcagctcca
     1861 gcgcgatgtt caaccgcttg cgctgcccgc cgctgatggt tttctgcagc ggcgagccca
     1921 ccttgaggtc cgcgatgtcc gcctgcccga ggtcggcgag catccgccgg acgcgcgcga
     1981 tccgtcgggc ctcgggcagg tgggccaggc agagccgggc ggcgtagtag aggttttcgt
     2041 ataccgtcag atcctcgaac agcatgtcgt cctgcggcac gaaaccgatc acgccctcgc
     2101 cggccccggc gtggatgtcc cggccgttca gccgcacgtc accggagctg ggccggaggg
     2161 tgccgttgag gatttccagc agcgtggact tgccggcgcc gctggcgccc atgatgccca
     2221 ccagccggcc gccccgttcg gcgaaggaga aatcgtgcag gccgcaccgg ccgtcggcga
     2281 accggtattc gaggcgggcg ccccggaaat ccaggaccgg gccggccccc tcctcgcgga
     2341 aagccgccgc gacggctgcg tagtggagtt cgtttcccca tcggtcgcgc aggacctggc
     2401 ccgggcagag cagccgggcc gctcccggcg ccagcggatg gccttccagc cagaccggct
     2461 cgtcgccggt ggcagccgcg agaaacaggt cgtcgccccg tagtttcagg accgcgagcc
     2521 ggccgcggaa gccggccgtt ccgccgccca tccaccggcc gtccgcaccg ggcggacagg
     2581 ttaggaaggc ccgcatgcgc ccggcctcct ccgccaggtc cagttcccgg ccaagccact
     2641 cggccatccg gccgggccag tccgagccgg ccgacacggc ggcgagttcc aggacgcaga
     2701 gtaccgccgc ataccgctcc tcgccgccca actgcgtctt cagcgacgcc acgatctccc
     2761 cggccgcgcg caaccggtcg gcctcgtccc cgatcgcgtg cagttcggcc aggtcctggt
     2821 acaggccgat atacacgccc gcgtccgcga ggccgacgtg gttgttcagg tagtccagcg
     2881 tccgccggcg ggcggcctcg cgggcttcgc cgcgcagcgg cgcggcggcc agcgcgaaga
     2941 ggtggagcag cccgttgagg acgggttcgg cgatcatggt ccgcctcggg atcagttccc
     3001 ggcgccggcg cgcaggatgg agcggcgcat cgcggcgatc tgctccccga tctcgttcca
     3061 gatggccgcc cctgtctccg gcgggatccg gccggcctgc tcatcctcta cgacctgggc
     3121 gatgctgcgg acaacgtcga gcttgccggg cgcgtcgcat tccagtccga gcgcggcggc
     3181 ctgccgctcg aaggcggcga aggcgcggag caccatcttc atccacggct cgcggccggg
     3241 ctgcgacggc tcctcgtaga gctgcgcgcg gagtttcccg gtggcgtcgt tgcgctggaa
     3301 gtagcccatg acgtgggcgg actcgatgga aaacccgtag agcgagtcca tcaggaagtg
     3361 cgccgtgacc gggctcgaca tggtgacggg gatttcgtcc gcgaggttgt tggcgagttc
     3421 gtcgagcagc gcctggacgt cgatcgcccc gggatcctcc gcggccttct ggagggtggc
     3481 gtacgagcgt ccgctgaaat cggcgtgcga gcgcaggtcc aggccggtga tgatctcctc
     3541 gagggcccgc atggcgtcgg ccgtttcctg ccgtttctgg aaggcgatgg cataaacggc
     3601 gtcgaagagc ttgatgccgg cgaactgctg ctgctggcag cgggtccagg ccggatcgat
     3661 cgcggcgggg gcgacgagat cgggctggta ggacaggccc agttcctgca ggcgcgcggt
     3721 ccggtcgaac atggaatgca cgttttcgcg gacgaggatt tcctggacct cggcggaaag
     3781 ctgcttcagg gcctccaccg ccgcctgcgg cgcggcctcc gacggggccg gcggggcctc
     3841 cgcaacggcc gcggttccac ccagcgcgag cgcgacatcg accagcagca
//
LOCUS       DAOOTK010000197         3859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_15103_length_3859_cov_3.930599, whole genome shotgun sequence.
ACCESSION   DAOOTK010000197 DAOOTK010000000
VERSION     DAOOTK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15103_length_3859_cov_3.930599"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="PK634_14095"
     CDS             <1..1238
                     /locus_tag="PK634_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98907.1"
                     /translation="QTGLEQQLARAIQEQDLFTLPESWMLMEFLERMSVKAPEEQREL
                     YTSLLPRDRKAYDDFRSGRLSSEWISSVNYEEDQAGAMLLAADPSKTEAYLRLVRSDS
                     MLNGFVEALVEEPRGYLWLYARRGAGSMVRFGFDQSGQLYLQVWRGGQIIARSTRYWV
                     KPDRPIRLRLELRGDGALGFVDGEAAFTAPLAIPRSMGLGWWGLAPWAPQFGQAQVLI
                     REVSGGPLPVQIGWFQSRGESLTDEQILARLKPHIQQISAVSPPWFAHASDGTVEKRG
                     EGDFPDVRIFSRYHQARLLPAIQDVSLRTLDVAQLVPLAETNRVDGFTLLVPRMPPED
                     WFERAEKDLLGTSLTLLVAHLDLDKKTAELREVAPYVGLFPGARATHTLPMIEPEATP
                     PDAPPKPQDENQESAVLIL"
     gene            1393..2817
                     /locus_tag="PK634_14100"
     CDS             1393..2817
                     /locus_tag="PK634_14100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOW98908.1"
                     /translation="MYVLDAPRPTVCPFCQRRMQQESFPARPPAENRAERNVEERLRR
                     LYHSPPRVSMLLMAAGAILLLAIVIYLQLINIEHYWYSPLVNIFSLVVGIFILTRFLF
                     AAFYTAPASVGFEPPVTVSIPCYNEEKSIAQSIAQIFAQDYPHWKMEVVVVNDGSRDN
                     TLEEMLKAQAAFPLLVIVDFERNRGLVYGMAAASLMAHSDFIVFVDSDTFLMPNAVRK
                     VIQGFVDPTVGGISGHTDVENSKVNLLTRMQDVRYYFSYTIMKAAESVFGMVSCLPGC
                     FSAYRRSCVLHVLDDWIHSTCFGKVGNFGDDRSLTNFVLKDYRILYDSEAVATTLAPE
                     NWRQYVRQQARWMRSYAREVFRASKFMWRKHPIPALSWYVMMWMPIVEPLVILQALVI
                     GPLLMGTVTMPYVVGVMAITLIWSMEHLRRTGRTDWWAGFFFTFSYALFFSWQIYYAL
                     ATLRRAAWGTRGTGTGTATGGGVP"
     gene            2814..>3859
                     /locus_tag="PK634_14105"
     CDS             2814..>3859
                     /locus_tag="PK634_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOW98909.1"
                     /translation="MSKRVPDRQPPPVHPKSTVPPPDSDARIRTMKASYLQAWLYFFL
                     VVSLLVAWWVPFHIWMLRKAAQRAQDRGPLDSYSFLALSYAGISDQPREVSPERFREH
                     IEALKARGYVTITLEEARRLIYEGRPVPRGALLMTFDQSRKSSYFDARSVLRRAGYHG
                     VMFIWTRPILEGEPSSLRWPYVRRMLRVGGWEIGAQSHDGFSLVPADAGGGEGHFLTT
                     PRWLAADQRYETLEQYAGRLAADHMQCLDIIEKQTGTRPRAFAFPYGDFGQYDDKAVL
                     TRRLNLDLVGQQYDLGFVLGSAALNTRDSDPRHLTRLMVQPDWTAEELVQRLRHAWPR
                     DEYSPEYLFKTPLA"
BASE COUNT          574 a         1380 c         1307 g          598 t
ORIGIN      
        1 cgcagacggg gctggagcag cagctggccc gcgcgatcca ggagcaggac ctgttcaccc
       61 tgccggaatc ctggatgctg atggagttcc tcgagcgcat gtcggtcaag gcccccgagg
      121 agcagcgtga actctacacc tcgctcctgc cccgcgaccg gaaggcctac gacgacttcc
      181 ggtcgggccg cctgtcctcg gaatggatca gctcggtgaa ctacgaggag gaccaggcgg
      241 gcgccatgtt gctggcggcc gacccgtcca agaccgaggc ctacctgcgg ctggtccgtt
      301 ccgacagcat gctcaacggg ttcgtcgagg cgctggtgga ggagccgcgg ggctacctgt
      361 ggctctacgc gcggcgcggc gcgggcagca tggtccgctt cgggttcgac cagtcgggcc
      421 agctctacct gcaggtctgg cgcggcgggc agatcatcgc ccgcagcacg cgctactggg
      481 tcaagccgga ccggccgatc cgcctgcgcc tggaactccg gggcgacggc gcgctgggct
      541 tcgtggacgg cgaggccgcg ttcaccgcgc cgctggcgat cccgcgcagc atggggctgg
      601 gctggtgggg cctcgccccg tgggcgccgc agttcggcca ggcccaggtg ctgatccgcg
      661 aggtcagcgg cgggccgctc ccggtgcaga tcggctggtt ccagagccgg ggcgagtcgc
      721 tgacggacga gcagatcctc gcccgcctca agccgcatat ccagcagatc tcggccgtgt
      781 cgccgccctg gttcgcgcac gcctccgacg ggacggtcga gaagcggggc gagggcgatt
      841 tccccgacgt gcggatcttc tcccgctacc accaggcgcg cctcctcccc gcgatccagg
      901 acgtatcgct gcgcaccctg gacgtggccc agctggtccc cctggcggag accaaccggg
      961 tggacggctt cacgctgctc gtgccccgca tgccgcccga ggactggttc gagcgggcgg
     1021 agaaggacct gctcggcacc tcgctgacgc tgctggtcgc gcacctggac ctggacaaga
     1081 agacggccga gctccgggag gtggcgccgt acgtcgggct gttcccgggc gcgcgcgcca
     1141 cgcacacgct gcccatgatc gagccggagg ccaccccgcc ggacgcgccg cccaagcccc
     1201 aggatgagaa ccaggagagc gccgtcctga tcctttgacg ccatgcccga aacatccagc
     1261 cgcccgggga ggacatcccg ccgcgcccgg aagcccggag ggcccgcgcg ggaccgcccg
     1321 aacgggccgg gggcgccttc cctggagccg aagaatggca cccgctgccg ctgcgaggac
     1381 tgcttcttga gcgtgtacgt gctcgacgcg ccccgcccga cggtctgccc cttctgccag
     1441 cgccgcatgc agcaggaatc cttcccggcc cggccgccgg cggagaaccg ggccgagcgg
     1501 aacgtcgagg agcgcctccg ccgcctctac cactccccgc cccgcgtctc gatgctcctg
     1561 atggccgccg gcgccatcct gctgctcgcg atcgtcatct acctgcagtt gatcaacatc
     1621 gagcactact ggtactcgcc gctggtcaac atcttcagcc tcgtggtggg gatcttcatc
     1681 ctgacgcgct tcctcttcgc cgccttctac acggcgccgg cgagcgtcgg gttcgagccc
     1741 ccggtgaccg tctccatccc ctgctacaac gaggagaaat ccatcgccca gtccatcgcg
     1801 cagatcttcg cgcaggacta cccgcactgg aagatggagg tcgtggtcgt caacgacggc
     1861 tcgcgggaca acaccctcga ggagatgctc aaggcgcagg ccgccttccc gctgctggtg
     1921 atcgtggact tcgagcgcaa ccgcgggctg gtctacggca tggcggcggc ctcgctgatg
     1981 gcccacagcg acttcatcgt cttcgtggat tccgacacct tcctgatgcc caacgccgtg
     2041 cgcaaggtca tccagggatt cgtcgacccg accgtgggcg ggatctccgg ccacacggac
     2101 gtggaaaact cgaaggtcaa cctgctgaca cggatgcagg acgtgcgcta ttatttctcg
     2161 tacacgatca tgaaggcggc ggagagcgtg ttcgggatgg tgagctgcct gccggggtgc
     2221 ttctcggcct accggcgctc gtgcgtgctg cacgtgctgg acgactggat ccacagcacc
     2281 tgcttcggga aggtcggcaa cttcggcgac gaccggagcc tgaccaactt cgtgctgaag
     2341 gactaccgga tcctgtacga cagcgaggcc gtcgcgacga ccctggcgcc ggagaactgg
     2401 aggcagtacg tacggcagca ggcgcgctgg atgcgctcct acgcgcgcga ggtgttccgg
     2461 gccagcaagt tcatgtggcg gaagcacccg atcccggcgc tgtcgtggta cgtgatgatg
     2521 tggatgccga tcgtggagcc gctggtcatc ctgcaggccc tcgtcatcgg gccgctgctg
     2581 atgggcacgg tgacgatgcc gtacgtggtc ggcgtgatgg ccatcaccct gatctggtcc
     2641 atggagcacc tgcggcgcac cgggcgcacg gactggtggg cgggcttctt cttcacgttt
     2701 tcctacgcgc tgttcttcag ctggcagatc tactacgccc tggccacgct gcgccgcgcg
     2761 gcctggggca cgcgcgggac cgggaccggc acggccaccg ggggaggcgt gccgtgagca
     2821 aacgcgtccc ggaccgccag ccgccgccgg tccacccgaa gtcgacggtc cccccgcccg
     2881 acagcgacgc ccgcatccgc acgatgaagg cgtcgtacct ccaggcgtgg ctctatttct
     2941 tcctcgtcgt cagcctgttg gtggcctggt gggtgccgtt ccacatctgg atgctccgga
     3001 aggccgccca gcgcgcgcag gaccgcgggc cgctcgactc gtactcgttc ctggcgctgt
     3061 cctacgccgg catctccgac cagccccgcg aggtgtcgcc cgagcgcttc cgggagcaca
     3121 tcgaggcgct gaaggcccgg ggctacgtga cgatcacgct ggaggaggcg cgccggctga
     3181 tctacgaggg ccggccggtc ccgcggggcg cgctcctgat gaccttcgac cagtcgcgca
     3241 agtcctccta cttcgacgcc cgcagcgtgc tgcggcgggc cgggtaccac ggcgtgatgt
     3301 tcatctggac gcggccgatc ctggaaggcg agccgtcgag cctgcgctgg ccctacgtcc
     3361 ggcggatgct gcgggtcggc ggctgggaga tcggcgccca gagccacgac ggcttcagcc
     3421 tggtgcccgc ggacgcgggc gggggcgagg gccacttcct gaccaccccg cgctggctgg
     3481 ccgccgacca gcgctacgag acgctcgagc agtacgcggg ccgcctggcc gccgaccaca
     3541 tgcaatgcct cgacatcatc gagaagcaga ccggcacacg gccccgcgcc ttcgccttcc
     3601 cctacggcga cttcgggcag tacgacgaca aggccgtgct gacccgccgc ctcaacctcg
     3661 acctcgtggg ccagcagtac gacctcggct tcgtcctcgg ctccgccgcg ctgaacacgc
     3721 gcgacagcga cccgcgccac ctgacgcgcc tgatggtgca gccggactgg accgcggagg
     3781 agctcgtcca gcgcctccgg catgcctggc cgcgcgacga gtacagcccg gagtacctct
     3841 tcaagacgcc gctggcctg
//
LOCUS       DAOOTK010000198         3820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_15314_length_3820_cov_4.728818, whole genome shotgun sequence.
ACCESSION   DAOOTK010000198 DAOOTK010000000
VERSION     DAOOTK010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15314_length_3820_cov_4.728818"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK634_14110"
     CDS             complement(<1..278)
                     /locus_tag="PK634_14110"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98910.1"
                     /translation="MEAGSLGEKVLDILLKRREIPAEPLREVRQEASTSGRRLEKLLV
                     EKNLVSALDMTLALAEHLKLPPITLTHFTPSPDLLQILPMELLAQHAML"
     gene            414..1247
                     /locus_tag="PK634_14115"
     CDS             414..1247
                     /locus_tag="PK634_14115"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98911.1"
                     /translation="MDKTKPEDAGDASNVREAIAAFEQILEALPDDRLALETLLDAYA
                     EIGDRPKTLQYLLRLARVVADEADAEAAPKVLDRLRELGAEHPAAVELTERLARLAAP
                     ATPAAAAEPVRRRAVDISPELALAWNLAQAGEISQEDYAAIAHDLSENSTRTVVVPVT
                     VQHVLYDRGFKQIDKILQFMSTNSGLPILPLGQFDVPREAYTLLPLDFMTRRAAMVFE
                     LMDSDALVVLLNPYDTELRDEVRRTIGRPCHFFLTTALAYDACLDRIRKAIEAEAESA
                     G"
     gene            complement(1252..1836)
                     /gene="pgsA"
                     /locus_tag="PK634_14120"
     CDS             complement(1252..1836)
                     /gene="pgsA"
                     /locus_tag="PK634_14120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOW98912.1"
                     /translation="MNLPNILTMSRLALAAVMMAFLSLEFPYSRTLALLVFSAAAITD
                     YFDGYLARRHYGITAFGQLMDPLTDKVLVCAAFVSFVEMQLVPAWIAVIIIGREFLVT
                     GLRLLAAHRGVVVSAGRWGKHKTVWQITAIVIVLLGLAVRQDYLAWAAPRILAGFDLW
                     FARFAYAISLAVAGITLVSGGLYFREHRDIIDAR"
     gene            complement(1833..2420)
                     /locus_tag="PK634_14125"
     CDS             complement(1833..2420)
                     /locus_tag="PK634_14125"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOW98913.1"
                     /translation="MPDIGQMFREARERKKVSCSQAASATRMKVQHVEALERNDFSRM
                     AAPMYAKGFIRLYADYLGLDPEPLIREYAAQQAPGKSPSLKPEERTFSGPSLADLVRR
                     HARQLWARVRPRLQAWRRPLLIAAVIVAAGVIGLALLRGRGRPAPAPAAPVEPPAAAP
                     ATPGLPLPLLREPPDAYLDGSKPLSERTPASGSAR"
     gene            complement(2426..>3820)
                     /locus_tag="PK634_14130"
     CDS             complement(2426..>3820)
                     /locus_tag="PK634_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOW98914.1"
                     /translation="KETMSEFGVEVDVTHVESGPVVTRYELLPAPGVRVERIVALSNN
                     IALAMKAESVRVQAPIPGKGVVGIEVPNSKTTMVCLREILESPAWKESSAALPLALGK
                     DVGGHDIVADLGDMPHMLIAGATGSGKTVCMNSILAGLLMSRTPDQMRLMLIDPKIVE
                     FSVYNHLPHLVVPVITDSKKVGIGLRWAINEMEKRYKLFAKVGVRNIHSFNRRPLAKQ
                     TELFPEGGGAETPPEEKVPDRLPYIVIIVDELADLMLVAQAEIELSITRLAQLSRAVG
                     IHMILATQRPSVNVITGTIKANFPARISFQVAQKVDSRTILDANGADKLLGKGDMLFL
                     PPGTSKLIRAQGTLTTDAEIRRLTEYWRKQGAPSYETAVKDKIEGKTADLPEMEEDDE
                     LLDQAIEVIRQTRRASTSSLQRRLRIGYTRAARLIDLLEQRGVVGPPCGSDPREILID
                     LDGEIPQNEPEEEA"
BASE COUNT          588 a         1303 c         1325 g          604 t
ORIGIN      
        1 agcatggcgt gctgggccag cagttccatc ggcaggatct ggagcagatc cgggctgggg
       61 gtgaaatgcg tcagggtgat gggcgggagc ttgaggtgct cggccagcgc cagggtcatg
      121 tcgagggcgg agaccaggtt cttctccacc agcagcttct cgagccggcg gccggacgtg
      181 ctcgcctcct ggcgcacctc gcgcaggggc tccgcgggga tctcccggcg tttcagcagg
      241 atatccagga ctttttcacc caggctgccg gcttccatga tgccttcctt cccgttccct
      301 gccttaaaag cagttggaat gccaaccgcc atccggcccg ccccttcgca ggcgctggat
      361 gacaaatggc cgcgcgtgta ttatataggg aaaggacccg gcaggaaagg cggatggaca
      421 agaccaagcc agaggacgcg ggggacgcct ccaacgtgcg ggaggccatc gccgccttcg
      481 agcagatcct cgaggcgctg ccggacgacc ggctcgcgct ggagaccctg ctggacgcct
      541 acgcggaaat cggcgaccgc cccaagaccc tccagtatct cctccgcctg gcgcgcgtcg
      601 tggccgacga ggcggatgcc gaggccgcgc cgaaggtgct ggaccggctg cgcgagctgg
      661 gcgcggagca tccggcggcc gtcgaactca cggagcgtct ggcccggctc gcagcgcccg
      721 cgacgcccgc ggccgccgcc gagcccgtcc ggcgccgcgc ggtcgacatc tctcccgaac
      781 tggcgctggc ctggaacctc gcgcaggccg gcgaaatttc ccaggaggac tatgccgcca
      841 tcgcgcacga cttgagcgag aactccacgc gcacggtggt ggtcccggtc acggtccagc
      901 acgtgctcta cgaccggggc ttcaagcaga tcgacaagat cctgcagttc atgtccacga
      961 acagcggcct gccgatcctc ccgctcggcc agttcgacgt gccgcgggag gcctacaccc
     1021 tgctgccgct ggatttcatg acgcgccgcg ccgcgatggt gttcgagctg atggattccg
     1081 acgcgctggt ggtcctgctc aatccctacg acacggagct gcgcgacgag gtgcggcgca
     1141 ccatcggccg cccctgccat ttctttctca ccacggcgct ggcgtacgac gcctgcctgg
     1201 accgcatccg caaggccatc gaggcggagg cggagagcgc cggctgatcc ctcaccgggc
     1261 gtcgatgatg tcccggtgct cgcggaagta gagcccgccg gagaccagcg tgatcccggc
     1321 gaccgccagc gagatcgcgt aggcgaagcg cgcgaaccac aggtcgaatc ccgccaggat
     1381 cctcggcgca gcccacgcca ggtagtcctg gcgcacggcc aggcccagca ggacgatcac
     1441 gatggcggtg atctgccaga cggtcttgtg cttgccccag cggccggccg aaacgaccac
     1501 cccccggtgc gcggccagca gccgcaggcc cgtcaccagg aactcgcggc cgatgatgat
     1561 cacggcgatc cacgcgggga cgagctgcat ctccacgaag cttacgaacg ccgcgcacac
     1621 cagcaccttg tccgtcagcg gatccatcag ctgcccgaac gccgtgatgc cgtagtggcg
     1681 ccgggccagg tagccgtcga agtagtcggt gatcgccgcg gcgctgaaca cgagaagggc
     1741 cagcgtccgc gagtacggga actccagcga caggaaagcc atcatcacgg cggccagcgc
     1801 caggcggctc atggtcagga tattgggcag gttcatcgcg ccgatccgga cgccggtgtc
     1861 cgctccgaca ggggcttgga gccgtccagg taggcgtccg gcggctcgcg aagcaagggc
     1921 agcggaagac ccggggttgc cggcgccgcg gcgggcggtt cgaccggcgc cgccggggcc
     1981 ggcgcgggac ggccccgccc gcggagcagc gccaggccga tcacccccgc cgccacgatg
     2041 acggcggcga tcagcagcgg ccgcctccag gcctggagcc gaggccggac ccgggcccag
     2101 agttgccgag cgtgccggcg gaccaggtcg gcgagcgacg gccccgagaa ggtccgctcc
     2161 tcgggcttga gcgaggggga tttccccggg gcctgctgcg cggcgtattc gcggatcagc
     2221 ggctccgggt ccagcccgag gtagtcggcg tacagccgga tgaagccctt ggcgtacatc
     2281 ggcgcggcca tgcgggagaa gtcgttccgc tccagggcct cgacgtgctg gaccttcatc
     2341 cgcgtcgcgg acgccgcctg ggagcaggac accttcttcc gctcgcgggc ctcgcggaac
     2401 atctgtccga tgtccggcat gccgatcagg cctcctcctc gggctcattc tgcgggattt
     2461 cgccgtccag atcgatgaga atctccctcg ggtcggaacc gcagggcggt ccgaccacgc
     2521 cccgctgctc gagcaggtcg atcaggcggg cggcccgcgt gtacccgatc cgcaggcggc
     2581 gctggagcga ggaggtcgac gcccggcggg tctggcggat gacctcgatg gcctggtcca
     2641 gcagttcgtc gtcctcctcc atttccggga ggtcggcggt cttgccttcg atcttgtcct
     2701 tgacggccgt ctcgtaactg ggggcgccct gcttccgcca gtattcggtc agccgccgga
     2761 tctccgcgtc cgtggtcagg gtgccctgcg cgcggatgag cttgctggtg cccggcggca
     2821 ggaacagcat gtcgcccttg cccagcagct tgtccgcccc gttggcgtcg aggatggtgc
     2881 ggctgtccac cttctgggcc acctggaagc tgatgcgcgc cgggaaattg gccttgatgg
     2941 tgccggtgat cacgttgacc gagggccgct gggtcgcgag gatcatgtgg atgccgacgg
     3001 cccgcgagag ctgggccaga cgggtgatgg agagctcgat ctccgcctgc gccacgagca
     3061 tgaggtcggc cagctcgtcg acgatgatca cgatgtacgg caggcggtcg gggaccttct
     3121 cctcgggcgg cgtctccgcg ccgccgcctt ccgggaacag ctccgtctgc ttcgccagcg
     3181 gccggcggtt gaacgagtgg atgttgcgca cgccgacctt ggcgaacagc ttgtaccgct
     3241 tctccatctc gttgatggcc cagcgcagcc cgatgccgac cttcttcgag tcggtgatca
     3301 cgggcacgac gaggtgcggc aggtgattat agacggagaa ctcgacgatc ttggggtcga
     3361 tcagcatcag gcgcatctgg tccggcgtgc ggctcatcag caggcccgcg aggatggagt
     3421 tcatgcagac ggtcttgccg gagccggtcg cgcccgcgat gagcatgtgc ggcatgtcgc
     3481 cgaggtcggc cacgatgtcg tgcccgccga cgtccttgcc gagggccagc ggcagggcgg
     3541 ccgacgattc cttccaggcg gggctctcca gtatctcccg caggcagacc atggtggtct
     3601 tggagttggg cacttcgatg ccgacgacgc ctttgccggg gatgggcgcc tgcacccgga
     3661 cgctctcggc cttcatggcc agggcaatat tgttgctcaa ggccacgatc cgctccaccc
     3721 gcacgccggg ggccggcagg agttcgtagc gcgtgaccac cggcccgctc tcgacgtggg
     3781 tgacgtccac ctcgacgccg aattccgaca tggtctcctt
//
LOCUS       DAOOTK010000199         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_15485_length_3789_cov_6.631762, whole genome shotgun sequence.
ACCESSION   DAOOTK010000199 DAOOTK010000000
VERSION     DAOOTK010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15485_length_3789_cov_6.631762"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK634_14135"
     CDS             complement(<1..724)
                     /locus_tag="PK634_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98915.1"
                     /translation="MNRWFLVFLLALGLLPPDASAASPRPGAVDAGDFFLTPKSSRPL
                     WRVAGMVALRAETGADGQALARQLIARGGPIQGYSLGQDVGHGYQLLAAPEAERIRLD
                     SEPGRLDAVLAAVRAQPGVSSFNPVFVDPDSGLKGLVTPDMIIRLKPGVEADAYFGAD
                     RDRVRPLRHTTDQFLLTLPITGAEDVLREINRHAGDPRVEWAQPDFLGEVRRAFTPSD
                     PLFTNQWHLGNTGQNGAKPAADV"
     gene            complement(717..2624)
                     /locus_tag="PK634_14140"
     CDS             complement(717..2624)
                     /locus_tag="PK634_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98916.1"
                     /translation="MPAGAQLPTLINYQGRLVSGTNLYSGAAQVVFRVYSDPTNTVMV
                     HYQDTDTVSVADGLYSTFIGDEGAASDMSWLSMSVPGFWLEVAVNGTALRPRERLVAV
                     PFSLLAGGVTNGAITTMMIADGSVMGYHLNNSSVDSTKIQDDSISFADMNQNGAANGQ
                     VMKWSGSAWVASDDNTGGGGGSLLGYREYGTFTPSPNAAGSDVIAMGKGCSAGGMNSI
                     VGGGAYNVALGAENAVVDGGAYNTNAGGGAVIGGGQYNRIAYEGSNAVVAGGWMNEID
                     GYKSAIGGGQGNRIQVRAAYSVIGGGDNNLVATGSYGCVVAGGLGNKVQPGIYQGFIG
                     AGGGNRMDDGDYGFIGAGQANRIGGDASWAVVGGGQYNTITNDADYAAIPGGFSNVAA
                     GAGAFAAGREAQALHAGAFVWNGSSSGSFHSTADGQFLVNAPGRVGINVMNPQADLHV
                     VGGADMGRLFVCPDALGTAGAELQLNESLSGNWGIALRYNFDSNQLEILGMTPSETNG
                     PHLVVTRDEGRVGIGTQNLETNYLLSVGGAIRCEEIVVETGWADFVFEPGYPLPPLND
                     VERHIAEHGRLPGLPSAAEVAARGAGVGETQAKLLQKIEELTLYTIRQQKEIRALQQE
                     LAELRRENSRE"
     gene            complement(3165..>3789)
                     /locus_tag="PK634_14145"
     CDS             complement(3165..>3789)
                     /locus_tag="PK634_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98917.1"
                     /translation="PLQLFQENFARGTMITLTPWEPAEIWPLVAAALRARPAVIAPFV
                     TRPAETVPDRAALKFAPATDAAKGFYALRRADPKRKSDGTIVLQESGVMYAFAEQALP
                     RIDQEGFNLNIFYVASAELFDALPAAERDAIYPEDLAREAMGITGFTLPTMYRWILSR
                     AGRERTLHPFMRGHYLGSGQAAAVLKEARLDGENQFKAITDYVRSRR"
BASE COUNT          579 a         1270 c         1264 g          676 t
ORIGIN      
        1 ccacgtcggc ggcgggcttg gccccgttct ggcccgtgtt gccgaggtgc cactggttag
       61 tgaacagggg atcggaaggc gtgaacgcgc gccggacctc gccgagaaag tccggctggg
      121 cccactcgac gcgcggatcc cccgcgtgcc ggttgatttc ccgcaggacg tcctcggccc
      181 cggtgatggg cagcgtcagg aggaactggt ccgtcgtatg ccgcagcggg cggacgcggt
      241 cgcggtcggc gccgaagtag gcgtccgctt ccacgcccgg cttgaggcgg atgatcatgt
      301 ccggcgtcac gagccccttg aggccgctgt ccgggtccac gaagacgggg ttgaacgagg
      361 acacgccggg ctgggcccgg acggctgcca gcacggcatc caggcggccc ggttcggagt
      421 ccagccggat tcgttccgcc tcgggcgcgg ccagcagttg gtacccgtgc ccaacatcct
      481 ggcccaggct gtagccctgg atcggcccgc cccgtgcgat cagttgccgc gcgagggcct
      541 gcccgtcggc gccggtctcc gcgcgcaacg cgaccattcc ggcgacccgc cacagggggc
      601 ggctgctctt cggcgtcagg aagaagtccc ccgcgtcgac tgcgcccggt cgcgggctgg
      661 ccgcgctcgc gtccggcggg agcaggccga gcgccagcag gaacacgagg aaccatctat
      721 tcacgggaat tctcccgccg cagctccgcg agttcctgtt gaagcgctcg gatctccttc
      781 tgctggcgga tggtgtacag ggtcagctcc tcgattttct ggagcagctt ggcctgggtt
      841 tcgccgacgc ccgcgccgcg cgccgctact tccgcggcgg agggcaggcc gggaagccgg
      901 ccatgttccg cgatgtggcg ctcgacgtcg ttcagcgggg ggaggggata gcccggctcg
      961 aacacgaagt cggcccagcc agtttccacg acgatctcct cgcagcggat cgcgccgccg
     1021 acggagagca ggtaattcgt ctccaggttc tgcgtgccga tgcccacgcg accctcgtcg
     1081 cgggtgacga cgaggtgagg cccattggtc tccgacgggg tcattccaag gatctcgagc
     1141 tggttcgagt cgaagttgta gcgaagcgcg atgccccagt tgcccgacaa ggactcgttg
     1201 agctgcagct ccgcgcccgc tgtgccgagc gcgtccgggc acacgaagag ccggcccatg
     1261 tccgcgccgc cgaccacgtg caggtcggcc tgcggattca tgacgttgat gcccacccgt
     1321 cccggcgcgt tcaccaggaa ctggccgtcc gccgtggagt ggaagctgcc ggaggacgag
     1381 ccgttccaga cgaacgcgcc ggcgtggaga gcttgcgctt cgcgtcccgc cgcgaacgcc
     1441 cccgcgccgg cggcgacgtt cgagaagccg ccggggatgg ccgcgtagtc ggcgtcgttg
     1501 gtgatcgtgt tgtactggcc gccgccgacg acagcccacg aggcgtcgcc gccgatccgg
     1561 ttggcctggc cggcgccgat gaatccgtag tcaccgtcgt ccatccggtt cccgccgccc
     1621 gcgccgatga agccctggta gatccccggt tggaccttgt tgcccaggcc gcctgcgacg
     1681 acgcagccgt aggagccggt ggcgaccagg ttgttgtcgc ccccgccgat gaccgagtac
     1741 gcggcgcgga cttggatccg gttcccctgt cctccgccga tggccgactt gtacccgtcg
     1801 atctcgttca tccagccgcc ggcgaccacg gcattgctgc cctcgtaggc gatgcggttg
     1861 tactgtcctc cgccgataac cgctcctccg ccggcattcg tattgtacgc cccgccgtcc
     1921 acgacggcat tctcggcgcc gagcgccacg ttgtaggccc cgccgcccac gatgctgttc
     1981 atgccccccg cgctgcatcc cttgcccatg gcgatcacat cgctgccggc cgcgttcggc
     2041 gagggggtga acgttccgta ttcccggtac ccgagcagcg acccgcctcc accgcccgta
     2101 ttgtcgtccg atgccaccca tgcgctgccg ctccacttca tgacctggcc atttgccgcg
     2161 ccgttctggt tcatgtcggc gaaggagatg ctgtcgtcct ggatcttcgt gctgtccacg
     2221 gagctgttgt tgaggtggta gcccatcacc gaaccgtccg cgatcatcat cgtggtgatg
     2281 gccccgttgg tcacgcctcc cgccagcagg gagaagggga cggcgaccag ccgttcgcgc
     2341 ggccggaggg ccgtgccgtt caccgcgact tcgagccaga agccgggcac gctcatcgac
     2401 aaccacgaca tgtcgctggc cgcgccctcg tccccgatga acgtggaata caggccgtcc
     2461 gcgaccgaga cggtatccgt gtcctggtaa tggaccatga ccgtattcgt gggatcggag
     2521 tacacgcgga atacgacctg cgccgctccg ctgtacaggt tggttccgct gacgagtcgg
     2581 ccctggtagt tgatcagcgt cgggagttgt gcgccggccg gcattccagt gagggccaca
     2641 catgcgataa ccaagctaca ggacgtgctt ttcatagcgg gcctcctgtc tccccggctc
     2701 cgcggggcaa cctcgtgcta tgaagccatt atcccgttcg tggccggtcg agtcaattgc
     2761 gccggcaaat ttaccggcag ggcgcgcgac gtaatccgtg acgacttggt tgcggtcgag
     2821 ctagggcccg ctccagaacg ggtgaagtat cacgtgaaga cccaggcatg ttgtccatac
     2881 tttgcatagt atgaaaatct ggcatgaacc gcgatggatg ttatatgcag tccagtagtt
     2941 ggatagaacc tattgcagct tcagaaatat ggttcaggaa tttccacact ttgcaaagtg
     3001 tggaaattat tcaaatcgaa caccaataga attgccgtgc aacttctcct gcgtgaacga
     3061 ccgggcatcg aaggcatggc gcggcattcc ccatgcccaa ggctgtccgt tttcaccgga
     3121 aggccggata tgtcattcct tcctggccgg cgaacggacg tccactaccg tcggcttcgc
     3181 acgtaatccg tgatggcctt gaactggttc tcgccgtcca ggcgcgcttc cttgagcacg
     3241 gcggcggcct ggccgctgcc gaggtagtgt ccgcgcatga acgggtggag cgtccgctcg
     3301 cgcccggccc gggagaggat ccaccggtac atcgtcggca gggtgaagcc ggtgatgccc
     3361 atggcctcgc gcgcgaggtc ctccgggtag atcgcgtcgc gttccgcggc ggggagcgcg
     3421 tcgaacagct cggcgctggc gacgtagaag atgttcaggt tgaagccttc ctggtcgatt
     3481 cgcggcaggg cctgctcggc gaaggcgtac atcacgccgc tctcctgcag gacgatcgta
     3541 ccgtcgctct tccgcttcgg gtcggcgcgg cgcagggcgt agaagccctt cgccgcgtcg
     3601 gtcgccgggg cgaacttcag cgcggcgcgg tcggggacgg tttccgccgg ccgcgtgacg
     3661 aacggcgcga tcaccgccgg gcgcgcgcgc aaggcggcgg cgacgagcgg ccagatttcc
     3721 gccggctccc acggcgtcag ggtgatcatc gtgccacggg cgaagttctc ctggaacagc
     3781 tggagcggc
//
LOCUS       DAOOTK010000200         3747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_15716_length_3747_cov_4.578819, whole genome shotgun sequence.
ACCESSION   DAOOTK010000200 DAOOTK010000000
VERSION     DAOOTK010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15716_length_3747_cov_4.578819"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PK634_14150"
     CDS             complement(<1..673)
                     /locus_tag="PK634_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOW98918.1"
                     /translation="MIQPGDTYVVMGLLDADSIAYAIGKGIEQRGGHVIYTVQNERLK
                     KIFLDRSDKLSEAEKSALRFEYCDVLDDQQVAGLFERTGPIAGVVHSIAYANPKTCLG
                     AEFHTDAVDDLKTAFHISCVSLATVARHAHRHMPDGGSIVAMTFESRLAFPYYNWMGV
                     NKAALEAVVRGLARRHGRDLIRVNAISSGPLTTKAAKAIPHFAYLARIWRKVSPLPWD
                     TIEDKT"
     gene            807..1109
                     /locus_tag="PK634_14155"
     CDS             807..1109
                     /locus_tag="PK634_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98919.1"
                     /translation="MAEKKGTGIKSAVELALERLAGRGDLPAGLSPEQKKALAEVDTQ
                     VRARVAEVEILMDGKMAAARAKPDPEELQKLAEQKQAEIRRIRERGERDKDAIRKG"
     gene            1237..3153
                     /gene="speA"
                     /locus_tag="PK634_14160"
     CDS             1237..3153
                     /gene="speA"
                     /locus_tag="PK634_14160"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOW98920.1"
                     /translation="MKPDALTRWTPEDSAELYGVRNWGAGYFDVADSGEVIVRPRGRG
                     GEVSISLMEIVSGLKARGLGMPVLLRFGDILASRVAQLNASFAKAMADAGYTAEYRGV
                     YPIKVNQQQQVVEAVVAFGRPFHHGLEAGSKAELIAALAYISDPEAFIVCNGYKDEEF
                     VELALHALKMGLQTVIVLEMADELPMVMERAKAIGVRPRLGVRVKLATRGGGHWTDSG
                     GDRSTFGLNASQIIDVVDWLRAENALDCLHMLHYHIGSQIPNIRSVRAAASEACRFYV
                     DLVKEGAKMGVLNVGGGLAVDYDGSHTNFQSSSNYAVDEYAADIVEVVMKVCNVAQVP
                     HPVLVSESGRATVAHHSVLLFNMLESTRFESHGLPDGLPPDAPEPLRQLMDVSASLTS
                     KNVQECYHDAVYYRDETRALHEVGSVSLRYRALAERIFWSIVTRIAAEIQGRKYVPDE
                     LSGLEVAIADVYYGNFSVFQSLPDVWAIDQLFPVMPVHRLKEAPTRQATISDITCDCD
                     GKIDRFIDLHDVRQTLPVHELNDSGEYYVGVFLVGAYQETLGDLHNLLGDTNVVNLRI
                     GEGGTVEYAHEIAGDTVADVLSYVEYNPQEMLDRMRKNAEQAVRAGRITVEERRQLMQ
                     AYEAGLRGYTYFEK"
     gene            complement(3167..>3747)
                     /locus_tag="PK634_14165"
     CDS             complement(3167..>3747)
                     /locus_tag="PK634_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98921.1"
                     /translation="SSAGYCDHINWRTGERSRYYSTDHQARLFLAMANYLERSLVWRT
                     FQSDPLARTGLRLKSECRSTEYRRTLMRKAIRSLSRKTPATFWWFATDSLMPVAGDLL
                     QASLWARSLSAQPLKGYSERWRLTDRAGRILGEGEEPFDLAPRETRKLTDIMIPTSRL
                     RPGVIWTLKSQLLQDGQELATMEKDIRFRRHD"
BASE COUNT          627 a         1229 c         1301 g          590 t
ORIGIN      
        1 cggtcttgtc ctcgatcgtg tcccacggca gggggctgac cttgcgccag atgcgcgcga
       61 ggtacgcgaa gtgcgggatc gccttcgccg ccttcgtggt cagcgggccg gaggagatgg
      121 cgttgacgcg gatcaggtcg cgcccgtggc gccgcgcgag cccgcgcacg acggcctcga
      181 gcgcggcctt gttcacgccc atccagttgt aatagggaaa ggccagccgc gactcgaagg
      241 tcatggcgac gatcgagccg ccgtccggca tgtgccggtg cgcatggcgc gccacggtgg
      301 cgagggacac gcagctgatg tggaaggcgg tcttgaggtc gtcgaccgcg tccgtgtgga
      361 attccgcgcc gaggcaggtc ttcgggttgg cgtaggcgat cgagtgcacg acgccggcga
      421 tgggcccggt ccgctcgaac aggcccgcca cctgctggtc gtccaggacg tcgcagtact
      481 cgaaccgcag ggcgctcttt tccgcctcgg acagcttgtc gctccggtcc aggaatattt
      541 tcttcagccg ctcgttctga acggtgtaga tgacatggcc gccccgctgc tcgatgccct
      601 tgccgatggc gtaggcgatc gaatccgcgt ccagcagtcc catcacgacg taggtgtcgc
      661 ccggttgaat catggcctgt tcctccgctt cctcgaaaaa ggagcataac ctaccgcgtt
      721 ctgcgaaaaa agcaaaaggc cgcgccgcgc ggttgattcc ggcggccggc caggtccata
      781 atgcgcctcc acgcggagga ggatgcatgg cggagaaaaa agggacgggc atcaagagcg
      841 cggtcgaact ggccctggag cggctggcgg ggcggggcga cctcccggcc gggctgtcgc
      901 cggagcagaa gaaggccctg gccgaggtgg atacccaggt gcgcgcccgg gtggccgagg
      961 tggaaatcct gatggacggg aagatggccg cggcccgggc caagccggac ccggaggagc
     1021 tgcagaagct ggcggagcag aagcaggccg agatccggcg catccgcgag cgcggcgagc
     1081 gcgacaagga cgccatccgc aagggatagc gtccggttcc ctccgcggat ccggtgtccg
     1141 gccggctgcc ggcgctattt ttcttccgcg catcgttgca ttttgcggcg gacgttctag
     1201 tattcgccgc gtggaatgcc aacaggagaa ggcggaatga aaccggatgc gctgacgcgc
     1261 tggacacccg aggattcggc ggaactgtat ggggtcagga actggggggc cgggtacttc
     1321 gatgtcgccg acagcggcga ggtgatcgtg cgcccgcgcg gccggggcgg cgaggtcagc
     1381 atcagcctca tggagatcgt ttccggcctg aaggcccgcg gcctgggcat gccggtgctc
     1441 ctccggttcg gcgacatcct ggcgtcccgc gtggcccagc tcaacgcgag cttcgccaag
     1501 gcgatggcgg acgcgggtta caccgccgag taccgcggcg tctacccgat caaggtcaac
     1561 cagcagcagc aagtggtgga ggccgtggtc gccttcggcc gccccttcca ccacggtctg
     1621 gaagccggca gcaaggcgga actgatcgcc gcgctggcct acataagcga tcccgaggcc
     1681 ttcatcgtct gcaacggcta caaggacgag gagttcgtcg aactggcgct gcacgcgctc
     1741 aagatgggcc tccagacggt catcgtgctc gagatggccg acgagctgcc gatggtgatg
     1801 gagcgcgcga aggccatcgg cgtccggccc cgcctcggcg tgcgcgtgaa gctcgcgacc
     1861 cgcggcggcg gccactggac ggattccggc ggcgaccgta gcaccttcgg gctcaacgcc
     1921 tcgcagatca tcgacgtggt ggactggctg cgcgcggaga acgcgctgga ttgcctgcac
     1981 atgctccact atcacatcgg ctcgcagatc ccgaacatcc gcagcgtgcg ggccgcggcg
     2041 tcggaggcct gccggttcta cgtggacctg gtcaaggaag gcgcgaagat gggcgtgctg
     2101 aacgtcggcg gcggcctggc cgtggactac gacggctcgc acacgaactt ccagagcagc
     2161 agcaactacg ccgtggacga gtacgcagcc gacatcgtcg aagtcgtcat gaaggtgtgc
     2221 aacgtcgccc aggtgccgca ccccgtgctc gtcagcgagt ccggccgcgc cacggtcgcc
     2281 caccactcgg tgctgctgtt caacatgctc gagtcgaccc ggttcgagag ccatggcctg
     2341 cccgacgggc tgcccccgga cgccccggag ccgctgcgcc agctcatgga cgtgagcgcc
     2401 tcgctgacct cgaagaacgt ccaggagtgt taccacgacg ccgtctatta ccgggacgag
     2461 acgcgcgccc tgcacgaggt cggctccgtg tcactccggt accgcgcgct ggcggagcgc
     2521 atcttctgga gcatcgtcac gcggatcgcc gccgaaatcc agggccgcaa gtacgtcccc
     2581 gacgagttga gcggcctcga ggtcgccatc gccgacgtgt actacgggaa cttcagcgtg
     2641 ttccagtccc tgccggacgt gtgggccatc gaccagctct tcccggtcat gcccgtgcac
     2701 cgcctcaagg aggcgccgac ccgccaggcc acgatctcgg acatcacctg cgactgcgac
     2761 ggcaagatcg accgcttcat cgacctgcac gacgtccgcc agacgctgcc cgtccacgaa
     2821 ctgaacgact ccggggagta ctacgtgggc gtctttctcg tcggcgcgta ccaggagacc
     2881 ctcggcgacc tgcataacct gctgggcgat acgaacgtcg tcaacctgcg catcggggag
     2941 ggcggcaccg tggagtacgc gcacgagatc gcgggcgata ccgtggccga cgtgctctcg
     3001 tacgtggagt acaacccgca ggagatgctc gaccggatgc gcaagaacgc cgagcaggcc
     3061 gtccgggccg gccgcatcac ggtcgaggag cgccgccagt tgatgcaggc gtacgaggcc
     3121 ggcctccgcg ggtacaccta cttcgagaag taaggcgttc cgccgttcag tcgtggcggc
     3181 ggaaccggat gtccttttcc atggtggcca gttcctggcc gtcctgcagc agctggctct
     3241 tgagcgtcca gatcacgccc ggccgcaggc gcgaggtcgg gatcatgata tcggtgagct
     3301 tgcgcgtctc gcgcggggcc aggtcgaaag gctcctcgcc ctcgcccagg atccgcccgg
     3361 cccggtcggt caggcgccac cgctcggagt atcctttcag cggctgcgcg gaaagcgagc
     3421 gcgcccagag ggacgcctgg agcaggtcgc ccgccaccgg catcagcgag tccgtggcga
     3481 accaccagaa ggtggcgggc gtcttgcggg acagcgaccg gatggccttg cgcatcaggg
     3541 tccggcggta ctccgtgctg cggcattccg atttcaggcg caggccggtc cgcgccaggg
     3601 gatcggactg gaaggtccgc cagaccaggc tgcgctccag gtagttggcc atcgccagga
     3661 acaggcgggc ctggtgatcc gtcgagtagt acctgctccg ctcgccggtc cgccagttga
     3721 tgtggtcgca gtacccggcc gacgact
//
LOCUS       DAOOTK010000201         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_16429_length_3624_cov_4.594284, whole genome shotgun sequence.
ACCESSION   DAOOTK010000201 DAOOTK010000000
VERSION     DAOOTK010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16429_length_3624_cov_4.594284"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PK634_14170"
     CDS             complement(<1..725)
                     /locus_tag="PK634_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOW98922.1"
                     /translation="MIRVTHLTKRFAGCVAVDDVSFRIEPGEVVGFLGVNGAGKTTTL
                     RILSCYLPATSGTAEVAGFDVFRDSLEVRRRIGYLPENAPLYPEMRVDEYLRFRARLK
                     GVSPAACRRRVQEVKDRCGLKDAGRRIIGQLSRGYRQRVGLADCLVHDPELLILDEPT
                     LGLDPAQVIQVRELIRALARRHTILLSTHILPEVEAVCRRVLIINRGRIVASDSPGQL
                     RENLQSGARAVTEIAGPADEVRRG"
     gene            complement(729..2096)
                     /locus_tag="PK634_14175"
     CDS             complement(729..2096)
                     /locus_tag="PK634_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOW98923.1"
                     /translation="MKTEWCKWIAAGAVALAWPTGGADASNRRTPIVEAVEKAMPGVV
                     NIGTEQLVTVRHTDPFRRFRGDLFDQFYRDFFAGPATPQVRHSLGSGIVIDPAGYILT
                     NFHVVERASRIRVTLGDDTTYDAVFLAGDEVNDLALLKIEPAGPLTPVEFARDDDLML
                     GETVIALGNPFGLAQTVTVGVLSAKNREARYQGEVLYRDILQTDAAVNPGSSGGPLLN
                     ADGEMIGVNVAIYREAQNIGFAVPVRRVRDLLARWLCPRLLGKRWLGFEADASNGVLR
                     VAHPEAMPELAVDERLAAGDVIRSVGGAPVRDLYDFHKALLAYGVGQTVDIGFEREGK
                     PAAVPVTVAALPKPAGEKLARRRLGLDLAEKPFGPEKGRAPFGNGVAISGVVPGGAGE
                     QVGLRPGLYVARINEMQINSLEDAGLALESVRTGDPLLLTVVNLEESGPYLIAQSSQV
                     RMNAE"
     gene            complement(2119..3039)
                     /locus_tag="PK634_14180"
     CDS             complement(2119..3039)
                     /locus_tag="PK634_14180"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOW98924.1"
                     /translation="MAATPPTVLVVGASGFVGGHALRHLCSLGWKVVGTRMRGKDGGL
                     RAFDIVRDRLADRVDEIMGEAGRPTHVVNCAAMAQPDRCFLERELSYQINVVGTIRLI
                     EDAFAIGARPICMTSGFAYDGVSGYYADDEPHAPINEYGRHKAEVEEYCAAHHPDTLH
                     YRLDKVIGDDPAEYQMFTEWYQWHLQGKPVVCIANQLFSPTNVRDVARAIQAGCERGL
                     TGVYNIANPEYFTREELARQFFRLLGLQARIVCRTQEELKFIDLRPEKTYLDSTKFQK
                     DADFRFTSVRETIRTFIERLKQDPARAGEK"
     gene            complement(3045..>3624)
                     /locus_tag="PK634_14185"
     CDS             complement(3045..>3624)
                     /locus_tag="PK634_14185"
                     /inference="COORDINATES: protein motif:HMM:NF020073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOW98925.1"
                     /translation="LWEEHIVYFTPQTCRWNLAIAGFKLVHYECFPYPFENSLVAVVQ
                     ADAAASEGAFPPASSLEPEFDRARRFVRGIEEQRRKARALFARYRAGGGRVALFGAGH
                     LACTYINILGLADVIDFVVDDHPKKKGLFMPGSRLPIRGSEALLTENVKLCLLSLSPE
                     AEDKVIGRNQAFLARGGVFSSINPSSKHALKL"
BASE COUNT          560 a         1263 c         1240 g          561 t
ORIGIN      
        1 ccccggcgga cctcgtccgc ggggcccgcg atctccgtca ccgcccgggc gccggactgc
       61 aggttctcgc gcaactgccc gggcgagtcc gaggcgacga tccggccgcg gttgatgatc
      121 agcacgcgcc ggcagacggc ttcgacctcg gggaggatgt gcgtggaaag caggatcgtg
      181 tgccgccggg ccagtgcccg gatcagctcg cgcacctgga tgacctgggc gggatccagc
      241 cccagcgtcg gctcgtccag gatcagcagt tcgggatcgt ggaccaggca gtcggccagg
      301 cccacgcgtt gccggtatcc ccgcgaaagc tgcccgatga tccgccggcc ggcgtccttc
      361 aagccgcagc gatccttgac ctcctgcacg cggcggcggc aggcggcggg cgagacgccc
      421 ttgaggcgcg cccggaagcg caggtactcg tccacccgca tctcggggta cagcggcgcg
      481 ttctccggca ggtagccgat gcgccggcgg acctccaggg aatcgcggaa gacgtcaaag
      541 cccgcgacct ccgccgtgcc cgacgtggcg gggaggtagc acgacaggat gcgcagcgtc
      601 gtggtcttgc ccgcgccgtt gacgcccagg aagccgacga cctcgccggg ctcgatgcgg
      661 aacgagacgt cgtccaccgc cacgcagccg gcgaaacgtt tcgtgagatg ggtgacccgg
      721 atcatgcgtc actccgcgtt catccggacc tgggaactct gggcgatcag gtagggcccg
      781 ctctcctcga ggttgacgac ggtcagcagg agcgggtcgc cggtccgtac ggactcgagc
      841 gccaggcccg cgtcctcgag cgagttgatc tgcatctcgt tgatgcgcgc cacgtagagc
      901 cccgggcgca ggccgacttg ctcccccgcg ccgcccggga cgacgccgga gatggccacg
      961 ccgttgccga acggcgcgcg ccccttctcg gggccgaacg gcttctccgc caggtccagg
     1021 cccaggcgcc gccgcgccag cttttcgccc gcgggtttcg ggagcgccgc cacggtcacg
     1081 gggacggccg ccggtttccc ctcgcgctcg aaaccgatgt ccaccgtctg ccccaccccg
     1141 tacgccagga gcgccttgtg aaaatcgtag aggtcgcgga cgggcgctcc gcccacggac
     1201 cggatgacgt cgccggccgc cagccgctcg tcgacggcca gttccggcat cgcctccggg
     1261 tgcgccacgc ggagcactcc gttggacgca tccgcctcga agcccagcca gcgcttgccc
     1321 agcaggcggg gacagagcca gcgcgccagc aggtcgcgca cccggcggac gggcaccgcg
     1381 aacccgatgt tctgggcctc gcggtagatc gccacgttca ccccgatcat ctcgccgtcg
     1441 gcgttcaaca acgggccgcc ggagctgccg ggattcaccg cggcgtccgt ctggaggatg
     1501 tcccggtaaa gcacctctcc ctggtaccgc gcctcgcggt tcttggccga gaggacgccg
     1561 acggtgaccg tctgcgccag gccgaacggg ttgcccagcg cgatgaccgt ctcgccgagc
     1621 atcaggtcgt cgtcgcgcgc gaattccacg ggggtcagcg gccctgccgg ctcgatcttc
     1681 agcagggcca ggtcgttgac ttcgtcgccg gcgagaaaga cggcgtcgta ggtggtgtcg
     1741 tccccgaggg tcacccggat gcgcgacgcc ctctcgacga cgtggaagtt ggtcaggata
     1801 tagccggcgg ggtcgatgac gatcccggag cccagggagt gccggacctg gggcgtcgcc
     1861 gggccggcga agaaatcgcg gtagaactgg tcgaacaggt ctccccggaa ccggcggaac
     1921 ggatcggtgt ggcgcacggt gaccagctgc tcggtgccga tgttcacgac gcccggcatg
     1981 gccttctcga ccgcctcgac gatgggcgtg cggcggttgg acgcgtccgc cccgccggtt
     2041 ggccacgcca gggccaccgc gcccgcggct atccacttgc accattccgt tttcatatcc
     2101 ggcggcaaca ggagggcttc acttttcccc ggcccgcgcc gggtcctgct tcaggcgctc
     2161 gatgaacgtg cggatcgttt cccgcacgga ggtaaaccgg aagtcggcgt ccttctggaa
     2221 tttcgtggaa tcgagatagg ttttctccgg ccgaaggtcg atgaacttca gttcctcctg
     2281 ggtccggcag acgatccggg cctgcaggcc gagcagccgg aagaactggc gggccagttc
     2341 ctcccgcgtg aaatattccg ggttggcgat gttgtacacc cccgtgagcc cgcgctcgca
     2401 accggcctgg atggcccggg ccacgtcgcg cacgttggtc ggcgagaata gctggttggc
     2461 gatgcacacg acgggcttgc cctggagatg ccattggtac cactcggtga acatctggta
     2521 ctcggccggg tcgtcgccga tgaccttgtc gagccggtag tgcagcgtgt ccgggtggtg
     2581 cgcggcgcag tattcctcga cctccgcctt gtggcggccg tactcgttga tcggcgcgtg
     2641 cggctcatcg tccgcgtagt agcccgagac gccgtcgtag gcgaaccccg aggtcatgca
     2701 gatcggccgc gcgccgatcg cgaaggcgtc ctcgatcagg cggatcgtcc cgaccacgtt
     2761 gatctggtag ctcaactcgc gctccaggaa acagcgatcg ggctgggcca tcgccgcgca
     2821 gttgaccacg tgggtgggcc gccctgcttc gcccatgatc tcgtcgacgc ggtccgccaa
     2881 gcggtcccgc acgatgtcga acgcgcgcag gccgccgtcc ttcccgcgca tgcgcgtgcc
     2941 gaccaccttc catccgaggc tgcacagatg ccgcagggca tgcccgccca cgaagcccga
     3001 ggcgccgacg acgagaacgg ttggaggggt ggcggccata cggttcacag tttcaacgcg
     3061 tgtttgctgg acggattgat cgaggaaaaa acgccgccgc gcgccaggaa ggcctggttc
     3121 cgcccgatca ccttgtcctc cgcctccggg ctcaagctca acaggcagag cttgacgttc
     3181 tccgtgagca gggcctccga gccccggatc ggcaggcgcg agcccggcat gaacagcccc
     3241 ttcttcttcg ggtggtcgtc cacgacgaaa tcgatgacat ccgccaggcc gaggatgttg
     3301 atgtaggtgc aggccaggtg cccggcgccg aagagcgcga cccgcccccc gcccgcccgg
     3361 tagcgcgcaa agagggcccg ggccttccgt ctctgctcct cgataccgcg gacaaaccgg
     3421 cgggcccggt cgaactccgg ctccagcgac gaggccggcg ggaacgcgcc ttccgacgcg
     3481 gcggcgtccg cctgcacgac ggcgacgagc gagttctcga agggatacgg gaagcactcg
     3541 tagtgcacca gcttgaagcc cgcaatggcc aggttccacc ggcacgtctg gggggtgaaa
     3601 tagacgatgt gctcctccca gagg
//
LOCUS       DAOOTK010000202         3460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_17612_length_3460_cov_4.731278, whole genome shotgun sequence.
ACCESSION   DAOOTK010000202 DAOOTK010000000
VERSION     DAOOTK010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17612_length_3460_cov_4.731278"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PK634_14190"
     CDS             complement(<1..281)
                     /locus_tag="PK634_14190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOW98926.1"
                     /translation="MMRSILAPFGLFLVLGFAAKAQPPAEPPAAPGETPAPPPEAAPA
                     AAPRVKLQTSMGDIVLELDPAKAPLSVENFLKYVDDGPYNGTIFHRAITG"
     gene            498..704
                     /gene="rpmE"
                     /locus_tag="PK634_14195"
     CDS             498..704
                     /gene="rpmE"
                     /locus_tag="PK634_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOW98927.1"
                     /translation="MKKDIHPEYGEATISCACGNVIKTRSTVREMHINTCSRCHPFFT
                     GQAKILDAEGRIERFRKRYAKPAR"
     gene            891..1973
                     /gene="prfA"
                     /locus_tag="PK634_14200"
     CDS             891..1973
                     /gene="prfA"
                     /locus_tag="PK634_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOW98928.1"
                     /translation="MLDRKYIEAWLARLAEIESLLADPATAANQRRFQELLQEHARLL
                     EIRRAADIYFRLQKEREDSEAMLRAEGGDPDLLEMARAEVERLDRELPPAERALLETV
                     LPSDPADSRNTIMEIRAGTGGEEAALFASDLYRMYTKYAERCGWKTGLIDASPSSLGG
                     YKEIIFSVEGRNVFRTLRYESGGHRVQRIPVTEAGGRIHTSAATVAVLPEAEEVDVEI
                     KPEELKIEYCRASGPGGQSVNKSDSAVRIVHLPTNTVVTCQDERSQQRNREKAMRVLR
                     ARILDQVRSAEQARLSNERKTQMGSGDRSERIRTYNFPQNRLTDHRINLTIYTLDRVM
                     EGGLEPVVEALREYDIGSRIQQLMKT"
     gene            1987..2850
                     /gene="prmC"
                     /locus_tag="PK634_14205"
     CDS             1987..2850
                     /gene="prmC"
                     /locus_tag="PK634_14205"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOW98929.1"
                     /translation="MSQPLVSDVLAVAVRRLESAGIDEARLKAEWALGRALGCRRLEL
                     PLHAREAMPLSVESMFEGHVRRLAESEPLQYVLGDAEFMGLVLKTDRRALIPRPETEL
                     LVETVLAHPALWASGRRPAIADIGTGTGCIVLALASRRPGARYLARDLRPEALALARE
                     NAAALGLAGRIEFEEGDLLEGVPPGSLDAVIANAPYVPTAEWAELDRSVRDFEPRSAL
                     DGGKDGLDVIGRLVPQAFTSLRSGGIVFLEVGRGQADAVRRRLEADRFASVEVRKDLG
                     GHDRVVCGART"
     gene            2847..3317
                     /locus_tag="PK634_14210"
     CDS             2847..3317
                     /locus_tag="PK634_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98930.1"
                     /translation="MKPYRKISGPLLGQRAAFGRKQQLAVAILLPFLFFVALMQWRWI
                     TQNKQSLFRVGASWYVERLPCELCGQMGFIPSADKPGERTLCPICQGRGGAYIRRVDN
                     RDVICPACGGMSRIWDEVNPPHFCERCGGRGLVRMDEESTNPVPDAPSPGGPVR"
     gene            3332..>3460
                     /locus_tag="PK634_14215"
     CDS             3332..>3460
                     /locus_tag="PK634_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOW98931.1"
                     /translation="MFDKLTGALQGLFKKWRGLGKLSERNVQDALREVRLALLEADV"
BASE COUNT          575 a         1122 c         1237 g          526 t
ORIGIN      
        1 ccggtgatgg cgcggtggaa gatcgtgccg ttgtagggcc cgtcgtccac gtacttcagg
       61 aagttttcga ccgagagcgg cgccttcgcg ggatccagtt cgagaacgat gtcgcccatg
      121 ctggtctgga gtttcacgcg gggcgcggcg gcgggggctg cttccggcgg cggggccgga
      181 gtctccccgg gcgcggcggg cggttcggca ggcggctggg ccttggcggc aaacccgagg
      241 acaagaaaca ggccgaacgg ggcaaggatg gacctcatca tggactcctg tgtttgtgcg
      301 ttgaaacgaa acaacggaac ttcccggcat tctacgttac cgggccgggg caggcaagtt
      361 ggacgagcgc ggaaaaacgc cgaaaaaacc gcaaaaaagg gccttgaact gcggcggggc
      421 ggacggtatg gtacacgctc ttcgtccgag aacccggccg cgcggggcgg ctggaacacg
      481 agggttgtga gagcgttatg aaaaaggata ttcatcccga gtacggggag gccacgattt
      541 cctgcgcgtg cgggaacgtg atcaagaccc gttccaccgt gcgcgaaatg cacatcaaca
      601 cgtgctcgcg ctgccacccg ttcttcacgg gccaggccaa gatcctggac gccgaggggc
      661 gcatcgagcg tttccgcaag cgctacgcca agcctgctcg ctagcttcct ttcgcgggcc
      721 agtcatgtcg cgggacacgg ctggcccgtt ttgttttccc ggccaatccc gaagacggat
      781 gaaccgcaga gttcgcagag ggcgccgagg cgagtccggc cggatggcga atccggtccg
      841 gtccttccgt ggcctctgtg tcctccgtgg cgaactcccc ggatgaagag atgctggacc
      901 gcaaatatat cgaggcctgg ctggcgcggc tggcggagat cgagtcgctg ctggccgatc
      961 ccgcgacggc cgccaaccag aggcggttcc aggaactgct gcaggagcac gcgcgcctgc
     1021 tggagatccg ccgcgccgcc gacatctact tccggctgca gaaggagcgc gaggacagcg
     1081 aggcgatgct ccgcgcggaa gggggcgatc cggacctgct ggaaatggcc cgcgccgaag
     1141 tcgagcgcct cgaccgcgag ctgccgccgg cggagcgcgc gctgctcgaa accgtgctgc
     1201 cgtccgatcc cgccgacagc cggaacacca tcatggaaat ccgggccggc accggcggcg
     1261 aggaggccgc gctcttcgcg agcgacctct accggatgta caccaagtac gccgagcgct
     1321 gcggctggaa gaccggcctg atcgacgcca gcccgtcgag cctcggcggc tacaaggaaa
     1381 tcatcttttc cgtcgagggc cggaacgtgt tccgcacgct gcgctacgag agcggcgggc
     1441 accgtgtcca gcgcatcccg gtgaccgagg ccggcgggcg catccatacc tccgcggcga
     1501 ccgtcgccgt gctgcccgag gccgaggagg tggacgtgga gatcaagccg gaggagctga
     1561 agatcgagta ctgccgggcc tccgggcccg gcggccagag cgtgaacaag tcggattccg
     1621 ccgtgcggat cgtccacctg cccaccaata ccgtggtcac gtgccaggac gagcggtccc
     1681 agcagcgcaa ccgcgagaag gccatgcgcg tgctgcgcgc ccgcatcctc gaccaggtcc
     1741 gcagcgccga acaggcccgc ctgtccaacg agcgcaagac gcagatgggt tcgggcgacc
     1801 gcagcgagcg gatccgcacc tacaatttcc cgcagaaccg cctgactgac caccggatca
     1861 acctgaccat ctacacgctg gaccgcgtca tggagggcgg gttggagccg gtggtcgagg
     1921 ccttgcgcga gtacgatatc ggatcgagaa tccagcaatt gatgaaaacc tgaccgggag
     1981 tcccgcgtgt cccaacccct cgtttccgac gtgctggccg tcgcggtgcg ccgcctcgaa
     2041 tccgccggca tcgacgaggc gcgactcaag gccgagtggg ccctgggccg cgcgctggga
     2101 tgccgccggc tggaactgcc gctccacgcc cgcgaggcca tgcccctgtc cgtcgagtcc
     2161 atgttcgagg gccatgtccg gcggctggcg gagagcgagc cgctgcagta cgtcctgggc
     2221 gacgcggagt tcatgggcct ggtcctgaaa acggaccgcc gggcgttgat tccccggccg
     2281 gaaaccgagt tgctcgtcga gacggttctg gcgcacccgg cgctgtgggc gtccggccgg
     2341 cggccggcca tcgccgacat cggcaccggg accggctgta tcgtcctggc gctggcctcc
     2401 aggcggcccg gggcgcggta tctcgcccgc gacctccggc ctgaagcgct ggccctcgcc
     2461 cgggagaatg cggcggcgct cgggctggca ggccggatcg agttcgagga aggcgacctg
     2521 ctggaaggcg tgccgccggg ctcgctggac gccgtgatcg ccaacgcgcc ctacgtgccc
     2581 acggcggagt gggcggagtt ggaccggtcc gtgcgcgact tcgaaccccg gtcggccctg
     2641 gacggcggga aggacgggct ggacgtgatc gggcgtctcg tgccgcaggc ctttacatcc
     2701 ttgcggtcgg gagggatagt atttctggaa gtgggccgcg ggcaggcgga cgcggtgcgc
     2761 cggcggctgg aagcggaccg gtttgcgtcc gtcgaggtcc ggaaggacct gggcgggcac
     2821 gatcgggtgg tttgcggggc caggacatga aaccgtacag gaaaatctcg ggaccgctcc
     2881 tgggacagcg cgccgccttc ggccggaagc agcaactggc cgtggcgatt ctgctgcctt
     2941 tcctgttttt cgtggccctc atgcagtggc gctggatcac gcagaacaag caatccctgt
     3001 tccgggtcgg ggcgtcctgg tacgtcgagc gcctgccgtg cgagctgtgc ggtcagatgg
     3061 gtttcattcc gtccgcggac aagcccggcg agcgcacgct gtgcccgatc tgccagggcc
     3121 gcgggggcgc gtacatccgg cgcgtggaca accgggacgt aatctgcccc gcctgtggcg
     3181 ggatgagccg gatctgggac gaggtgaatc ccccccattt ctgcgaacgg tgcggcggcc
     3241 gggggctcgt tcggatggac gaggaatcca cgaaccctgt ccccgacgcc ccgtcaccag
     3301 gcgggccggt tcgctgaccc gacccacgcc catgttcgac aagcttacag gggccctgca
     3361 ggggctgttc aaaaagtggc gcggcctggg caagctctcg gagcgcaacg tccaggacgc
     3421 gctgcgggag gtccggctgg ccctgctcga ggccgacgtc
//
LOCUS       DAOOTK010000203         3374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_18288_length_3374_cov_3.577282, whole genome shotgun sequence.
ACCESSION   DAOOTK010000203 DAOOTK010000000
VERSION     DAOOTK010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18288_length_3374_cov_3.577282"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..957)
                     /locus_tag="PK634_14220"
     CDS             complement(19..957)
                     /locus_tag="PK634_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98932.1"
                     /translation="MKAIRFVTLCVLAASLAGPAYPAAEPKSRQLTLLVVPARYNVLQ
                     VSFDAASISPVVLVSYQGDATTKTPLLHAWNGTEWVYVSAEDFREAQFLQITPGQAIL
                     VGDEGLLPPMLVSSVGAWCPKTLTVPATDPAGLVNALGAHLDFSPSDWKWMAARYNMN
                     LTDLNAERRSESWYDQKGYEDEMTPRLQSLPKHRRGRPAAGSVSPAARPAVVPPAEVV
                     PPAEEIPPAKAAPAAEEVPPAEIVTEVESASVEDIKAEEPAPAAEPRLSIPSPGEEPK
                     KGRKSRAEKKADEAAATEPAPEGWQEKASTDPLPVK"
     gene            complement(1051..1545)
                     /locus_tag="PK634_14225"
     CDS             complement(1051..1545)
                     /locus_tag="PK634_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOW98933.1"
                     /translation="MISGKMTKAINEQINKELYSSYLYLAMSAQAEALGLKGFANWFK
                     VQAAEENEHAMKFFDYLGEQGARVKLAAIDQPPVEFKSPKAMFEETLKHEKTVTASIN
                     ALADLALAEKDHATGSFLRWFVDEQVEEEANAQEIVDWLKLAGEAGNALLFLDSKLRA
                     RKAE"
     gene            1691..2194
                     /gene="tpx"
                     /locus_tag="PK634_14230"
     CDS             1691..2194
                     /gene="tpx"
                     /locus_tag="PK634_14230"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOW98934.1"
                     /translation="MATITLKGAAIHTAGELPRPGAKAPGFKLTGADLADVGLDQFAG
                     KKKILNIVPSLDTGVCATSAQKFSQEVSKLGGVALLNISADLPFAAARFCESHSLKNV
                     VTLSTFRSPAFGQDYGVAIVDGPLAGLMSRAVLVLDAQDRVIHAQQVPEIVQEPDYAA
                     VLNAVKK"
     gene            complement(2205..2624)
                     /locus_tag="PK634_14235"
     CDS             complement(2205..2624)
                     /locus_tag="PK634_14235"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOW98935.1"
                     /translation="MSTPALHSVRKGRQIAVFLDDEPGTLSGIAALLGRHGVNIYALS
                     LAEGIGHGYVRMVVDQPDAALKALREADELVMEREVLLLELANVPGALGEVTRRLAEA
                     KINLEYAYCAGGPNVDRGLVIVRVDHTERALAILQGR"
     gene            complement(2634..>3374)
                     /locus_tag="PK634_14240"
     CDS             complement(2634..>3374)
                     /locus_tag="PK634_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOW98936.1"
                     /translation="GFLPDLKKIGADVLKRTKLLYVNYPNNPTGAAAGPEFFDSLIAF
                     AQKHNILIVQDAAYATLIYGRERLSILGRPGGKDVAVELHSMSKSYNMTGWRLGFVAG
                     AAGAVKAFAEVKDNTDSGQFKAIQHAACAGIADRQLSEAIRAHYEKRLKLLVAALRRA
                     GFDAEMPGGTFYLYVPAPKAGGKLGFKTAEDASQYLLREHSVSTVPWDDVGAFLRFSA
                     TFESRGDADDKRVIAELEKRLAAAALQF"
BASE COUNT          515 a         1148 c         1123 g          588 t
ORIGIN      
        1 aaacgcgccg gccgatcatc acttgaccgg gagcggatcg gtggacgcct tctcctgcca
       61 gccctcgggg gcaggctcgg tcgccgcggc ctcatcggcc ttcttctccg cgcggctctt
      121 ccgccccttc ttcggctcct cgccgggcga cgggatggac aggcgcggct ccgcagccgg
      181 cgccggctcc tcggccttga tatcctcgac cgacgccgat tcgacttccg tgacgatctc
      241 ggcgggcggc acctcctcgg cggcgggcgc ggccttggcg gggggaatct cttccgcggg
      301 cggcaccacc tcggccggcg gcacgaccgc cggcctggcc gcgggggaca cggagccggc
      361 ggcgggccgg ccgcgccggt gcttcggcag cgactgcagc cgcggggtca tttcatcctc
      421 gtagcccttc tggtcatacc agctctcgct gcggcgctcg gcgttgagat ccgtcaggtt
      481 catgttgtag cgcgccgcca tccacttcca gtccgacggg gaaaaatcca ggtgggcgcc
      541 gagggcgttc acgaggccag cgggatcggt cgcgggcacg gtcagcgtct tcggacacca
      601 ggccccgacc gagctcacga gcatcggggg cagcaggccc tcgtcgccga cgaggatggc
      661 ctggccgggc gtgatctgca ggaactgggc ctcgcggaag tcctccgcgg agacgtaaac
      721 ccattccgtg ccgttccagg cgtgcagcag cggggtcttg gtcgtggcgt cgccctggta
      781 ggacacgagc accacgggcg agatggacgc ggcgtcgaac gagacctgca ggacgttgta
      841 ccgcgccggc accacgagca gggtcagttg cctggacttg ggttcggccg ccgggtaggc
      901 cggtccggcc aggctcgccg cgagaacgca cagcgtcacg aatcggatag ctttcatacc
      961 tcgccctctt tcagtgaaag gaacggaaac agactagcac ccccctcccg aggagtgcaa
     1021 gccgtagatg aacccgcggc cgtacggcgg ctattcggcc ttgcgcgccc ggagcttgga
     1081 atccaggaac aggagcgcgt tgccggcctc gcccgcgagc ttcagccagt ccacgatctc
     1141 ctgggcgttg gcctcctcct cgacctgctc gtccacgaac cagcgcagga agctgcccgt
     1201 cgcatggtcc ttctccgcta gcgcgaggtc cgccagggcg ttgatgctgg cggtgacggt
     1261 tttctcgtgc ttgagcgtct cctcgaacat ggccttcggc gacttgaact ccacgggcgg
     1321 ctggtcgatc gcggcgagct tgacgcgcgc gccctgctcg cccaggtaat cgaaaaactt
     1381 catcgcgtgc tcgttttcct cggcggcctg gaccttgaac cagttggcga accccttcag
     1441 ccccagggcc tccgcctgcg ccgacatcgc caggtacaga taggacgagt acagctcctt
     1501 gttgatctgc tcgttgatcg ccttcgtcat tttaccgctg atcatgttca ttcctccgtg
     1561 atgggattgc ccgtttgaat ttcaatcata cctcatgatc gccccccgcg ccagatggaa
     1621 cctgtttttt tcgcgcgccg ggttgcgccg gggccgatcg gcgttatatt ggcggaaaac
     1681 ggagggatcc atggctacca tcaccttgaa gggcgcggcg attcatacgg cgggcgaact
     1741 gcccaggccg ggcgcgaagg cgccgggatt caagctcacc ggggcggatc tggccgacgt
     1801 cgggctggac caattcgcgg gcaagaaaaa gatcctgaac atcgtgccga gcctggacac
     1861 cggcgtttgc gcgacgtcgg cgcagaagtt cagccaggaa gtctcgaagc tgggcggcgt
     1921 ggcgctgctg aatatttccg cggatctgcc gttcgcggcg gcgcgcttct gcgagagcca
     1981 ctcgctgaaa aacgtcgtga cgctgtccac cttccgctcc ccggccttcg gccaggacta
     2041 cggcgtggcc atcgtggacg gcccgctggc cgggctgatg agccgcgcgg tgctggtcct
     2101 ggacgcgcag gaccgcgtga tccacgccca gcaggtgccg gagatcgtcc aggagccgga
     2161 ttacgcggcg gtgctcaacg ccgtaaagaa ataagaggta cgcgtcagcg gccctggaga
     2221 atggccagcg cccgctcggt atggtccacg cgcacgatga ccaggccgcg atccacgttg
     2281 gggccgccgg cgcagtaggc gtattccagg ttgatcttcg cctcggcgag gcggcgcgtc
     2341 acctcgccca gggcgccggg gacgttcgcg agttccagca ggaggacctc ccgctccatc
     2401 accagttcat cggcttcccg cagcgccttc agggcggcat cgggctggtc caccaccatg
     2461 cgcacgtagc cgtgcccgat gccctcggca agggacaacg cgtagatgtt cacgccgtgc
     2521 cggccgagca gggcggcaat ccccgagagc gtgccgggct cgtcgtcgag gaacacggcg
     2581 atctgccggc ctttccggac gctgtgcagt gcgggtgtgc tcatgtaggc ctttcagaat
     2641 tgcagggccg ccgcggccag ccgtttttcc agttccgcga tcacgcgctt gtcgtccgcg
     2701 tcgccccgcg actcgaacgt cgccgagaac cgcaggaacg ccccgacgtc gtcccacggc
     2761 accgtggaca ccgaatgctc gcgcagcagg tattgcgacg cgtcctcggc cgtcttgaaa
     2821 cccagtttcc cgccggcctt cggcgcgggg acgtacaggt agaacgtccc gcccggcatc
     2881 tcggcgtcga agcccgcgcg ccgcagcgcc gcgacgagca gcttcagcct cttctcgtag
     2941 tgcgcgcgga tcgcctcgga gagctgccgg tccgcgatcc cggcgcaggc ggcgtgctgg
     3001 atggccttga actggcccga gtccgtgttg tccttgacct cggcgaaggc cttcacggcg
     3061 ccggcggcgc ccgcgacgaa gcccagccgc cagccggtca tgttgtagct cttgctcatc
     3121 gagtgcagct cgaccgccac atccttgccg ccgggccggc cgaggatgga aagccgctcg
     3181 cgcccgtaga tcagcgtcgc ataggccgca tcctgcacga tgaggatgtt gtgcttctgc
     3241 gcgaaggcga tgaggctgtc gaagaactcc ggcccggccg cggcgccggt cggattgttc
     3301 gggtagttca cgtagagcag cttggtcctc ttcagcacgt ccgcgccgat cttcttcagg
     3361 tcgggcagga agcc
//
LOCUS       DAOOTK010000204         3256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_19298_length_3256_cov_4.117776, whole genome shotgun sequence.
ACCESSION   DAOOTK010000204 DAOOTK010000000
VERSION     DAOOTK010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19298_length_3256_cov_4.117776"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PK634_14245"
     CDS             complement(<1..1336)
                     /locus_tag="PK634_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98937.1"
                     /translation="MLAAGRAQASDAEWDWGFFASRLEDAGGDVRTRALGPLLERADS
                     PDGGRLAAVRPVFCRARDGRGERRFSEIAWPLATTRRFLDERDTRVLLAYYKRFNVEE
                     TAGRYRFMIIPLYFQGRDKLGQNYRALFPLAGRIHEFLGQDEMAFFLFPLWSYSSINS
                     VRTDNVLWPVYSRTKGKGISRFRVFPFYGYARHRARFEKGFVGWPFYTWARYTYPDAQ
                     GRGYIVFPLWGHFKMPDQEAWLFLPPLFRFSKGERMDYSYCPWPFFQRSRGQIERFYL
                     WPLWGRRTMPGLRSSFFLWPVFRSERSDRGAVVRRRFWALPFVYAQVDRRRAAGDVPA
                     RTVARHHRLWPLCSYRQDETARRLRVLELWPLWDASPVERNWAPLWTLFSWTKIGQNA
                     DTEVLWGLARDRVRGAERRHVSLFPLWEWRRNDRPGGGREWNVGKGLIGRRRD"
     gene            complement(1486..>3256)
                     /locus_tag="PK634_14250"
     CDS             complement(1486..>3256)
                     /locus_tag="PK634_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOW98938.1"
                     /translation="LIQQVFNHRFREDENIPRALRDPLWVLPPGLRGICPPELAAGLR
                     ALGGYVSPGGGGPAIETGKHCLGNLLLASALFRAAGGRTAVPPGSRALRNGLDQVARL
                     IGAPPGRVHGATATPGELVFRYANGVEVTGQHKSSHARRGFAIDWMRADFTAEPDVPA
                     AVLRHLREADLIIIAPGSLYTSNIALLQVPGIAEAIRSNRRALKILGANFWVQEGETD
                     ISPHNAHNGFLVSELIEAYDRNVAGGASGLFDVVLASNLEHLPGDILRNYALEGKKPI
                     HLDRARVEAMGYRPVEAMLFAVDRSPYTNAVQHDPEKFAVAIRALLVADGLRQDVRLP
                     PGRRGAGNRVELPGPDRRPLAPCRYMEAMRRELRSKQFEPAGLRDILMDLAWKNRDIQ
                     PEHARLIRAVRIVPASRWNRSVEWDSVLGYFDAEDLTIKLHASLLRRPAQLREDLVIA
                     LGEAVLGRYIEERSWSEDPGVASHAARCYSIRLLPPAKRRTFLDPAQLTRYLRLARMN
                     RCPGDPGNWCITVNGDEGFLPPGLLFGLMYAWYLYNAWGGAMEYEMSLLHWPRGDLIP
                     CQARERERKQALVDFFRTEVFRR"
BASE COUNT          533 a         1164 c         1132 g          427 t
ORIGIN      
        1 cgtcccgccg ccggccgatc aagcccttcc cgacgttcca ctcccgcccg cccccggggc
       61 ggtcgttgcg gcgccactcc cacaggggga aaagcgacac gtggcgccgt tccgcgccgc
      121 gcacccggtc gcgcgcgagg ccccatagta cttcggtgtc cgcgttttgg ccaatcttcg
      181 tccacgagaa aagcgtccat agcggcgccc agttccgctc cacggggctg gcgtcccaga
      241 gcggccacag ttccaggacg cgcagccgcc gggcggtctc gtcctgccgg taggaacaca
      301 acggccagag ccggtgatgg cgggccacag tccgcgcggg cacatcgccc gccgcccgcc
      361 ggcggtccac ctgcgcataa acgaacggca gcgcccagaa ccggcgccgg accacggcgc
      421 cgcggtcgct ccgctcgctc cggaagaccg gccagaggaa aaacgaggag cggaggccgg
      481 gcatcgtgcg gcgcccccag agcggccaga ggtagaaccg ctcgatctgg ccccggctgc
      541 gctggaaaaa cggccagggg cagtaagagt agtccatccg ctcgcccttc gagaagcgga
      601 acagcggcgg caggaacagc cacgcctcct ggtcgggcat cttgaagtgg ccccacaggg
      661 gaaagacgat gtacccccgg ccctgcgcgt ccgggtaggt gtagcgcgcc cacgtgtaga
      721 acggccaccc cacgaatccc ttctcgaagc gggcgcggtg ccgcgcgtag ccgtagaacg
      781 ggaacacgcg gaaccgcgag atccccttgc ccttggtgcg cgagtaaacc ggccacagca
      841 cgttgtccgt gcgcacgctg ttgatcgacg aatagctcca gagcgggaag aggaagaacg
      901 ccatctcgtc ctggcccagg aactcgtgga tgcggcccgc gaggggaaac agcgcgcggt
      961 agttctgccc gagtttgtcg cggccctgaa agtacagcgg gatgatcatg aaccggtagc
     1021 gacctgcggt ttcttccacg ttgaaccgct tgtagtaggc caggagcacg cgggtgtcgc
     1081 gctcgtccag gaaccgccgc gtggtggcca gcggccaggc gatctcgctg aaccgccgct
     1141 cgccgcgccc gtcccgcgcc cggcagaaaa cggggcggac cgcggccagg cgcccgccgt
     1201 ccggcgaatc cgcgcgctcg agcagcggcc ccagcgcgcg cgtgcgcaca tcgccgccgg
     1261 cgtcctcgag gcggctggcg aagaagcccc agtcccactc ggcgtcggaa gcctgcgccc
     1321 ggccggccgc gagcaagagg gccagcgcga gggccgccgc ccggccccgc ggccctgtcc
     1381 cacgtttatg caaaggcggg ccaagctcgg gaagcggcgg gccggcggac gcgtcgccgc
     1441 cgcgaacaac gttctggaga atcgccgggc gcatggatcg ccggctcacc gccggaacac
     1501 ttccgtccgg aagaaatcca ccagcgcctg cttgcgctcg cgctcgcggg cctggcacgg
     1561 gatcaggtcg ccgcgcggcc agtgcagcag ggacatctcg tactccatcg cgccgcccca
     1621 ggcattgtac aggtaccagg cgtacatcag gccgaagagc aggccgggcg gcaggaagcc
     1681 ctcgtccccg ttgacggtga tgcaccagtt ccccgggtcg cccgggcagc ggttcatccg
     1741 cgcgagccga agataccggg tcaactgggc gggatcgagg aaggtcctcc gcttcgcggg
     1801 cggaagcagg cggatggaat agcagcgcgc cgcgtgggac gcgacgccgg gatcctccga
     1861 ccaggagcgc tcctcaatat agcggccgag cacggcctcc ccgagcgcga tgaccaagtc
     1921 ctcgcgcaac tgcgcgggac ggcgaagcag gctggcgtgc agcttgatgg tcaggtcctc
     1981 ggcatcgaaa tagcccagca cgctgtccca ctccacgctg cggttccagc gggaggccgg
     2041 gacgatccgc accgctcgga tgaggcgcgc atgctccggc tggatgtccc ggtttttcca
     2101 cgccaggtcc atcaggatat cccggaggcc cgcgggctcg aactgcttag agcgcaactc
     2161 gcggcgcatg gcttccatgt accggcacgg cgcgagcggc ctccggtccg gcccgggaag
     2221 ttcaacccgg ttccccgcgc cccgccggcc gggcggcagc cggacgtcct gccgcaggcc
     2281 atccgcgacc agcagcgccc ggatcgccac ggcgaacttc tccgggtcgt gctgcaccgc
     2341 gttggtgtac gggctgcgat cgacggcgaa gagcatggcc tccaccggcc ggtatcccat
     2401 ggcctccacg cgcgcccggt ccaggtggat gggcttcttg ccctcgaggg cgtagttgcg
     2461 caggatatcg cccggcaggt gctcgaggtt cgaggccagg accacgtcga aaagtccgct
     2521 cgcgccgccc gccacgttgc ggtcataggc ctcgatcaac tcggaaacca ggaagccgtt
     2581 gtgcgcgttg tgcggggaga tgtccgtctc gccctcctgc acccagaaat tcgcgccgag
     2641 gatcttgagc gcccgccggt tcgagcggat cgcctccgcg atccccggca cctggagcag
     2701 cgcgatgttg ctcgtgtaga ggctgccggg cgcgataatg atcaggtcgg cctcgcgcag
     2761 atgccgcaga acggccgcgg gcacgtccgg ctcggcggtg aaatcggccc gcatccagtc
     2821 gatcgcgaag ccgcggcgcg cgtgagagga cttgtgctgc cccgtgacct ccacgccgtt
     2881 ggcgtagcgg aaaaccaact cgcccggcgt cgcggtggcc ccatgcacgc gacccggcgg
     2941 ggcgccgatg agccgggcca cctggtccaa tccgttgcgg agcgcccgcg agcccggcgg
     3001 aacggccgtc cggccgccgg ccgcgcggaa cagcgcggac gcgaggagga gattgcccag
     3061 gcagtgcttg ccggtctcga tcgccggccc gccgcccccg ggcgacacgt agccgcccag
     3121 cgcgcgcagg cccgcggcca gttccggcgg gcagatgcct cgcaacccgg ggggcagcac
     3181 ccacaacgga tcgcgaaggg cgcgggggat gttttcgtcc tcccggaagc gatggttgaa
     3241 cacctgctgg atgagg
//
LOCUS       DAOOTK010000205         3199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_19813_length_3199_cov_5.049618, whole genome shotgun sequence.
ACCESSION   DAOOTK010000205 DAOOTK010000000
VERSION     DAOOTK010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19813_length_3199_cov_5.049618"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2782
                     /locus_tag="PK634_14255"
     CDS             <1..2782
                     /locus_tag="PK634_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98939.1"
                     /translation="LVRGTSTTNGWLLGGTRADATTGRAKYGVIAHANQSVATRSGTQ
                     GHIYTDRSDINLHPGFVRLLDSAEMLNTPVNLHISGTLLMSFLWAAQNPSESGYPNRD
                     GPTFLNRVKTFVTTGPGGLVGGVLAEHIMPYFEGEVNAKSIEQNSELIEHLFGLSESD
                     MKVMHVPERVIHTDTNSAHVDPAGPLDGKTFEEIAATDFTATYLDEVTHLHWWFYSGE
                     QTNAGWDTANCGRWAGGQGNDEEAYHHKVHKINGVFCFMINDREDQSKFGNSDGGMMN
                     DTRYTLLQKARDADSSKLTLVFDDWEAFAGNSFASATPNNNADQFHATLRWAANHPWI
                     EFVKLGTVAGWAASDASWVIDHGYVYDKSNQNYEWLKRASEHDYDHWYYGSAQEESFY
                     SRVPVLHDSWSPGGMKMYGDLNTPGTLMRDSWDAIQSIADSDQLKKIAEWSYSAMIYE
                     TAWHDEDANPDQYKSRNYQATFNRTTAEGNCDDSYEDTTWDDTSDWATRLHGHVRDMG
                     VMADAAGWVADVRSGAQGPRATVYAADVDDDTLDEYILCNNKVYLCFERWGARLVKAF
                     VYDASIGSGDGRMVVGAPISNPPEESENEGADNNRCSAFKDRYSTGQTNAYVDMDFAF
                     SAPVAGSNSWTFVSSDGRIAKKITLLNGRDVAVAEYGLAAAVGTLYTRFGLGPNQLDL
                     MFNGTQNLQRVSDPSFRGLLNTQGGEAYMVRGDNCGAITTAISNAGWDNRELPLTEQF
                     EAYNTATGFSVALAFSQSSARDVDGDGLANTNEWGIGSQHMKADSDGDGIPDGYETGN
                     GLAVLVADGTGDLDEDGALNYEEYVMNTGADDSNDVLRLEQAGVASGAFRIEHPVSGP
                     RQYQVLFADGLGAGWNWQAFGNTNAPVGSWATTNAGAARHVFTDDFTAATSGTMPTNG
                     PRVYTIRVSVPE"
     gene            2792..>3199
                     /locus_tag="PK634_14260"
     CDS             2792..>3199
                     /locus_tag="PK634_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98940.1"
                     /translation="MKRAILLAGLWVAAAQAGPVSDNAAFYYWKAVGLLRPARTPAQL
                     ELLRFAEEDLPLLPPRILEARPSVRNWLLSEQAVGEALAQASACPECDFGARQRSGPF
                     LDVAHLPRLAALCRRVCAMAQALDFAGREEEAAQ"
BASE COUNT          580 a         1035 c         1145 g          439 t
ORIGIN      
        1 gctggtccgc ggcacgtcga cgaccaacgg ctggctgctg ggcggcacgc gggcggacgc
       61 gacgaccggg cgtgcgaagt acggggtgat cgcccacgcg aaccagtccg tggccacgcg
      121 cagcgggacg caggggcaca tctacaccga ccgcagcgac atcaatctgc atcccggttt
      181 cgtgcggctc ctggactcgg cggagatgct gaacacgccg gtcaacctgc acatcagcgg
      241 cacgctgctg atgtcgttcc tgtgggccgc gcagaacccg tcggaatccg gctacccgaa
      301 ccgcgacggg ccgacgttcc tgaaccgcgt gaagaccttc gtcacgacgg ggccgggcgg
      361 gcttgtcggc ggggtgctgg cggagcacat catgccgtac ttcgagggcg aggtgaacgc
      421 gaagagcatc gagcagaaca gcgagctgat cgagcacctg ttcgggctct ccgagagcga
      481 catgaaggtc atgcacgtgc ccgagcgcgt catccacacc gacacgaaca gcgcgcacgt
      541 ggatcccgcc ggcccgctcg acggcaagac gttcgaggag atcgcggcca ccgatttcac
      601 ggcgacgtac ctcgacgagg tgacgcacct gcactggtgg ttctactccg gcgagcagac
      661 gaacgcgggc tgggacacgg ccaactgcgg gcgctgggcg ggcgggcagg ggaacgacga
      721 ggaggcctac caccacaagg tccacaagat caacggcgtg ttctgcttca tgatcaacga
      781 ccgcgaagac cagtcgaagt tcggcaacag cgacggcggg atgatgaacg acacgcgcta
      841 tacgttgctg cagaaggcgc gggacgcgga ctcgtccaag ctgacgctgg tcttcgacga
      901 ctgggaggcg ttcgcaggca actcgttcgc gtcggccacg ccgaacaaca acgcggacca
      961 gttccacgcc accctgcgct gggcggcgaa ccatccgtgg atcgagttcg tcaagttggg
     1021 cacggtcgcg ggctgggcgg cgtcggacgc gagttgggtg atcgaccacg ggtacgtcta
     1081 cgacaagtcc aaccagaact acgagtggct caagcgggcg agcgagcacg actacgacca
     1141 ctggtattac gggagcgcgc aggaggagag cttctacagc cgcgtgcctg tgctgcacga
     1201 cagctggtcg ccgggcggga tgaagatgta cggcgacttg aacacgccgg gcacgctgat
     1261 gcgcgactcg tgggacgcga tccagtccat cgcggactcg gaccagctga agaagatcgc
     1321 cgagtggagc tacagcgcga tgatctacga gacggcctgg cacgacgagg acgccaaccc
     1381 ggaccagtac aagtcgcgga actaccaggc gaccttcaac cggacgacgg cggagggcaa
     1441 ctgcgatgat tcctacgagg ataccacctg ggacgacacg tcggactggg cgacccggct
     1501 gcacgggcac gtccgcgaca tgggcgtgat ggcggacgcg gccggctggg tcgccgacgt
     1561 ccgcagcggc gcgcagggcc cgcgcgcgac ggtgtacgcc gcggacgtgg acgacgacac
     1621 gctggacgag tacatcctct gcaacaacaa ggtctacctg tgcttcgagc gctggggcgc
     1681 gcggctggtc aaggccttcg tgtacgacgc ctcgatcggc agcggcgacg ggcggatggt
     1741 cgtcggcgcg ccgatcagca atccgcccga ggagagcgag aacgaggggg cggacaacaa
     1801 ccggtgcagc gcgttcaagg accggtacag caccgggcag accaacgcgt acgtggacat
     1861 ggatttcgcg ttctcggcgc cggtcgcggg ctccaactcg tggacgttcg tctcgagcga
     1921 cgggcgcatc gcgaagaaaa tcaccctgct gaacgggcgc gacgtggccg tggccgagta
     1981 cgggctggcg gcggccgtcg gcacgctcta cacgcgattc gggctcgggc cgaaccagct
     2041 ggacctgatg ttcaacggca cgcagaacct tcagcgggtt tccgacccga gcttccgggg
     2101 gctgctcaac acgcagggcg gcgaggcgta catggtgcgg ggcgacaact gcggcgccat
     2161 caccaccgcg atctccaacg ccggctggga caaccgcgag ctgccgctga ccgagcagtt
     2221 cgaggcctac aacacggcga ccgggttcag cgtggccctc gccttcagcc agtcctccgc
     2281 gcgggacgtg gacggcgacg ggctcgcgaa caccaacgag tgggggatcg gcagccagca
     2341 catgaaggcc gacagcgacg gcgacggcat cccggacggg tacgaaaccg ggaacgggct
     2401 ggcggtcctc gtggcggacg ggacgggcga cctggacgag gacggcgcgt tgaactacga
     2461 ggaatacgtg atgaacacgg gggccgacga ctcgaacgac gtgctgcggc tggagcaggc
     2521 gggcgtggcg tccggcgcgt tccgcatcga gcatcccgtt tccgggccgc ggcagtacca
     2581 ggtgctcttc gccgacggcc tgggggcggg ctggaactgg caggccttcg gcaacaccaa
     2641 cgcgcccgtc ggatcctggg caacgaccaa cgcgggcgcc gcgcggcatg tcttcacgga
     2701 cgacttcacc gcggcgacct ccggcacgat gccgacgaac ggcccgcgcg tgtatacgat
     2761 ccgcgtgtcc gtgccggaat agggccgcac gatgaaacgg gccattctgc tggcggggtt
     2821 gtgggtcgcg gcagcgcagg ccggcccggt gtccgacaac gcggccttct actactggaa
     2881 ggccgtcggc ctcctgcggc cggcccggac gccggcgcag ctggagttgc tgcgcttcgc
     2941 ggaggaggat ttgccccttc ttccgccccg gatcctcgag gcgcggcctt ccgtgcggaa
     3001 ctggctgctc tccgagcagg ccgtgggaga ggcgctggcc caagcctccg cctgcccgga
     3061 gtgcgatttc ggcgcccggc agcgcagcgg gcccttcctg gatgtcgccc atcttccgcg
     3121 gctggctgcg ctctgccggc gggtgtgcgc catggcccag gccctggact ttgccggccg
     3181 cgaagaggaa gccgcgcag
//
LOCUS       DAOOTK010000206         3195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_19853_length_3195_cov_4.711146, whole genome shotgun sequence.
ACCESSION   DAOOTK010000206 DAOOTK010000000
VERSION     DAOOTK010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19853_length_3195_cov_4.711146"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK634_14265"
     CDS             complement(<1..256)
                     /locus_tag="PK634_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98941.1"
                     /translation="MSNPRLLVICHGFPPYHGGAELAAGYLAREAARDGWAVDVLTSD
                     LGGRLPAEETRDGAAIRRIRAPKKEWTRHTAAELGRFYLAA"
     gene            complement(249..1178)
                     /locus_tag="PK634_14270"
     CDS             complement(249..1178)
                     /locus_tag="PK634_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOW98942.1"
                     /translation="MKRSSHLAVIVPVFNEREAVGPVLEALVRFAENRDMEIIAVDDG
                     SDDGTDKVLAGLGERVIVRRHSSNRGYGASLKTGILATQARNVLFFDSDGQHDVADLP
                     RLLEELERHECVFGARPPGAGIPLVRKPGKWLLQHVCQFLAGRKIPDLNCGLRAGRRL
                     LFMRMLDLLPEGFSFSITSLMFVIKSRHSYVFVPVHCGPRVGKSSVRIFSDGIKAVLL
                     ALRLVMLFDPLRVLGVPAVALMTVGAVYQLYEIMAFRLKIVGGAILAILAGVILFLFG
                     LLADQIASLRKEISSHNSLFWELEQQRGKNGDE"
     gene            1323..2693
                     /locus_tag="PK634_14275"
     CDS             1323..2693
                     /locus_tag="PK634_14275"
                     /inference="COORDINATES: protein motif:HMM:NF003761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOW98943.1"
                     /translation="MNTCTLKKGMNIPLGGAPEAVLAEGGPAARITLYPNEFEGIKPR
                     PAVKVGDAVKRGTILYYSKRHAALTFRSPAAGTVEELALGHRRMLERIVIRFGASDDV
                     EPLSRFEAGRIGSLSREQILAALLDTGYLALLKQRPFSRIPDPAARPKSIFVNAMNTA
                     PFQPDAAVIVRGQETAFQAGLDAMTRLTAGKVRLCLPGGRADLPPALTAAKNVEIHYF
                     AGPHPAGNSSVHIHHLDPIRPHDVVWTVRAVDLVQIGRLLLDGAMPPDRVVTLAGPGV
                     REGERRYYRVRLGAPIEALLQGRLAPGEQRIVAGDVLAGKRVAPDSALRFQDSGICVL
                     PEGRERHFLGWIAPGWNRFSWSPTFLSTWLRRGAQWALDTSRRGSPRAMVLTGLYDRY
                     VPMRIMTDYLLRAVLARDTDEAVKLGLLEVDPEDFALCAFACPSKMDLVGIIRRGLAE
                     AEKEGI"
     gene            2774..>3195
                     /locus_tag="PK634_14280"
     CDS             2774..>3195
                     /locus_tag="PK634_14280"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOW98944.1"
                     /translation="MKIAEQMLDRMRPLFAPGRPLHLFHAVFDATDTFLLTPPDVTDG
                     PPHVRDAVDMKRVMMFVVIALIPCTLFGVFNAGYMYNTVNGVPGAGFVQHVLRGLRLV
                     LPIIITSYAVGGFWEVVFATVRKHEINEGFLVTGLLFPL"
BASE COUNT          520 a         1040 c         1122 g          513 t
ORIGIN      
        1 gcgcggcgag gtagaagcgg ccgagttccg cggccgtgtg ccgggtccac tccttcttgg
       61 gggcgcggat gcgccggatg gccgcgccgt cgcgcgtctc ctccgcgggc agtcgtccgc
      121 cgaggtcgga ggtcagcacg tccacggccc agccgtcgcg cgcggcctcg cgggcgagat
      181 acccggcggc cagttccgcg ccgccgtgat aggggggaaa tccgtggcag atcaccagga
      241 ggcgcgggtt actcatcccc gttttttccg cgttgctgtt cgagttccca gaagaggctg
      301 ttgtgagaac tgatttcctt gcgcaggctg gcgatctggt ccgcgagcag cccgaacagg
      361 aacaggatca cgccggccag gatggcgagg atcgcgccgc cgacgatctt caggcggaag
      421 gccatgatct cgtagagctg gtatacggcg ccgacggtca tcagcgccac ggccggcacg
      481 ccgaggacgc gcaggggatc gaacagcatc accagccgga gcgccagcag cacggccttg
      541 atgccgtcgc tgaaaatgcg gaccgagctc ttgccgacgc gcggcccgca gtggacgggc
      601 acgaagacgt aggagtggcg gctcttgatg acgaacatca gcgaggtgat ggaaaacgag
      661 aacccctccg gcagcaggtc cagcatgcgc atgaacagca ggcgccgccc ggcgcgcagg
      721 ccgcagttga ggtcggggat tttccggccc gccaggaact ggcaaacgtg ctgcagcagc
      781 cacttgccgg gcttccggac gagcgggatg ccggcgccgg gaggccgcgc gccgaagacg
      841 cattcgtgcc gctcgagctc ctccagcagc ctcggcaggt cggcgacatc atgctgaccg
      901 tcgctgtcga agaagagcac gttccgcgcc tgggtagcca ggatgccggt tttcagcgag
      961 gccccgtatc cgcggttcga ggagtgccgc cggacgatca cccgttcgcc caaccccgcc
     1021 agcaccttgt ccgtgccgtc gtcggagccg tcgtccaccg cgatgatttc catgtcgcgg
     1081 ttttcggcga agcggacgag ggcttccagt acgggcccca ccgcttcccg ctcgttgaac
     1141 acgggcacga tcacggccag atgactcgat cgcttcatgg catcgccccg ggggaaagat
     1201 aaggcaacaa gggccgccgc ggcaagggaa agcggccggc gggagggggg cccgttgact
     1261 tttcaagggc agcttgtaga gtactgcgga gtctctgacc ggctggacga cgccgggcct
     1321 tcatgaacac gtgcacgctt aaaaagggca tgaatatacc gctgggcggg gcgccggagg
     1381 ccgttctggc cgaggggggg cccgcggcgc gaatcaccct ttacccgaac gagtttgagg
     1441 ggatcaagcc ccggcccgcg gtcaaggtcg gcgacgcggt gaagcgcggc accatcctat
     1501 attatagtaa gaggcacgcc gccctcacat tccgctcgcc ggccgcggga accgtcgagg
     1561 aacttgcgct ggggcatcgc cgcatgctgg agcgcatcgt gatccggttc ggcgcgtcgg
     1621 acgacgtgga gccgctgtcc cgcttcgagg ccgggcggat cgggagcttg tcccgcgagc
     1681 agattctggc ggcgctgctg gatacgggct atctcgcctt gctgaagcag aggccgttct
     1741 cccgcattcc cgacccggcc gcccggccaa aatccatttt tgtcaacgcc atgaacacgg
     1801 cgccgttcca gccggacgcg gccgtgatcg tccgcggaca ggaaaccgca ttccaggccg
     1861 gcctggacgc gatgacccgg ctcacggccg ggaaggtgcg gctgtgcctg cccggcggcc
     1921 gggccgacct gcccccggcg ctgaccgccg cgaagaacgt ggagatacat tacttcgcag
     1981 gcccgcatcc cgcgggcaat tccagcgtgc atatccacca cctcgacccg attcgcccgc
     2041 acgacgtggt ctggacggtc agggccgtgg acctcgtgca gatcgggcgg ctcctgctcg
     2101 acggcgccat gccgccggac cgcgtcgtca cgctggcggg gcccggcgtg cgcgagggcg
     2161 aacgtcgcta ttatcgggtc cggctcggcg cgccgatcga ggcgctgctc cagggccgcc
     2221 tcgcgcccgg ggagcagcgg atcgtcgcgg gcgacgtgct ggccggcaag cgcgtggcgc
     2281 cggacagcgc gctccggttc caggattcgg gaatctgcgt cctgcccgag ggccgggagc
     2341 ggcatttcct gggctggatc gcgccgggct ggaaccggtt cagctggtcg ccgactttcc
     2401 tctccacctg gctgcggcgc ggggcgcagt gggcgctgga cacgagccgc cgcggcagtc
     2461 cgcgggccat ggtgctgacc ggcctgtacg accgctacgt tccgatgcgg atcatgaccg
     2521 attacctcct gcgcgcggtg ctggcgcgcg acacggacga ggccgtgaaa ctgggcctcc
     2581 tggaggtcga tcccgaggac ttcgcgctgt gcgcgttcgc ctgcccgtcg aagatggacc
     2641 tcgtcggcat catccgccgc ggcctggccg aggcggagaa ggaagggatt tgaacggaag
     2701 gcgcgaaggg cgcgaagacg gaagagcagg agcaggagca agaagctgaa tcctgtaccc
     2761 tagaaatgct tcgatgaaaa ttgcggaaca gatgctggac aggatgcgtc cgctgtttgc
     2821 gccgggcagg ccgctgcatc ttttccatgc cgtgttcgac gcgacggaca ccttcctgct
     2881 taccccgccg gacgtgacgg acgggccgcc gcacgtccgg gacgcggtgg acatgaagcg
     2941 ggtgatgatg ttcgtggtca tcgccctgat cccctgcaca ctgtttggcg tcttcaacgc
     3001 tgggtatatg tacaacacgg tcaacggggt gcccggggct ggcttcgtgc agcacgttct
     3061 ccggggcctg cgcctcgtgc ttccgatcat catcacgtcg tacgccgtcg gcgggttctg
     3121 ggaggtcgta ttcgccacgg tgcgcaagca cgagatcaac gagggcttcc tggtgaccgg
     3181 cctgctgttc ccgct
//
LOCUS       DAOOTK010000207         3056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_21245_length_3056_cov_4.766078, whole genome shotgun sequence.
ACCESSION   DAOOTK010000207 DAOOTK010000000
VERSION     DAOOTK010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21245_length_3056_cov_4.766078"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PK634_14285"
     CDS             <1..1495
                     /locus_tag="PK634_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOW98945.1"
                     /translation="RYFGSCIFSNSWLSNGLTTVDFIQIPSGVPDDRAVLEEFTVGYT
                     RLLAATGGVLSFAGRPGTNNFTVTRLASNESAWALDVTAPAAPVLLTNMAVSDVAGGR
                     TIRFGAVTAGSNLYYVCSTSAVRGVTGIERVMFRNLADTNRQADFIAVCPYEFRDGTY
                     RLLEHRYLTGLKVAVAPVSDIYNEFSYGIKDADAVKQFIGYAFHHWRAPEPGYVLFVG
                     DGSYDPKNNKGVVTNFDWVPVHLGPSAYDWSAQDSWFAAVNGNDFLADVGLGRLPVSG
                     ATDLTGIVAKIVAYDGLAATNSWRKKALLVADANDGINDFDADSDSVVDPHLLSNGFS
                     RTKAYRDSMTAPQVLGSITNSINGKVLLVSYFGHGAPDYWSADQFFVNADVNGLNNSV
                     WPLFLMLTCRNGMFDDAAKYTISESLLRRPNRGAIACAAASGLSVPAAANYFADGFTA
                     SLVNTQQQARVGDLMNAGFLNLWTVSPNQPELLFYSIIGDPAQVVNP"
     gene            1508..2320
                     /locus_tag="PK634_14290"
     CDS             1508..2320
                     /locus_tag="PK634_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98946.1"
                     /translation="MNFGRNNRAWRLAVAGCLFACRVLADDHGDAAVAATALVPDGGA
                     VAGVINDDVDKDWFSFSTLPWVGYSITVATGTLWDSEADLYASDGATCQRATNSAGWA
                     TPLTVAWSNSGARTKRHLEIGGFAEFTTGTYSVAVARLNWVDADGDGMFDPWEVEMLG
                     STNQPAGGDPDNDGFPNIDEYYLQTDPDDADSGLFVVRLTPAATGTVVRWDAAQNGTY
                     RLWSSTNLSSAAPGWTFFGEKTKFDASPAWDELVDPAATNARRQFYRVEFTY"
     gene            2421..>3056
                     /locus_tag="PK634_14295"
     CDS             2421..>3056
                     /locus_tag="PK634_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOW98947.1"
                     /translation="MKAFQNIIETVGRTPLVKLQRLSEETGAEIFIKMESHNPMGSVK
                     DRIGVAMINEAEKAGLIKANTVIVEPTSGNTGIALAFVAAARKYKLVLTMPESMSLER
                     RAMLRALGADLMLTPAEQGMRGAIAKASELVEKTPGAWMPQQFENPANPAIHEATTGP
                     EIWESSGGKIDAIVAGVGTGGTITGVARFLRKQNPAFQAFAVEPADSPVISG"
BASE COUNT          558 a          954 c         1001 g          543 t
ORIGIN      
        1 gcgatatttc ggcagctgca tcttttccaa cagctggctg tcgaacggcc tgaccaccgt
       61 ggatttcatt cagatcccat ccggcgtccc cgacgatcgg gcggtactgg aggaattcac
      121 cgtcggctat acccgtctgc tcgcggccac ggggggcgtg ttgagctttg ccgggcggcc
      181 gggcaccaac aacttcacgg tcacccgcct cgccagcaac gagtcggcct gggccctgga
      241 tgtgacggcg ccggcggccc cggttttgct gaccaacatg gccgtttccg acgtggcggg
      301 cgggcggacg atccgcttcg gcgccgtcac cgcggggtcc aacctgtact atgtctgttc
      361 cacctccgcg gtccggggcg tgaccgggat cgagcgggtg atgttccgca acctggccga
      421 cacgaaccgg caggcggact tcatcgccgt ctgcccgtac gaattcaggg acgggaccta
      481 caggctcctc gagcatcgct acctgacggg cctgaaggtg gccgtcgcgc cggtctcgga
      541 catctacaac gagttcagct acggcatcaa ggacgcggac gccgtcaagc agttcatcgg
      601 ctacgccttc caccactggc gcgccccgga gcccggctac gtgcttttcg tgggggacgg
      661 gagctacgac ccgaagaaca acaagggcgt cgtgaccaac ttcgactggg tgcccgtgca
      721 tctcggcccg agcgcctacg actggtcggc gcaggattcc tggtttgccg ccgtcaacgg
      781 gaacgacttc ctggcggatg tcggcctggg ccggctcccg gtgtccggcg ccaccgattt
      841 gacggggatc gtggcgaaga tcgtcgcgta cgacggcctt gccgcgacga actcctggag
      901 gaaaaaagcg ctcctggtcg cggatgccaa cgacggcatc aacgatttcg acgcggacag
      961 cgacagcgtg gtggacccgc acctgctctc caatgggttc tcgaggacca aggcgtaccg
     1021 ggacagtatg acggcccccc aggtgctcgg ctccatcacc aacagcatca acgggaaggt
     1081 tctcctggtt tcgtacttcg ggcacggcgc tcccgattac tggtcggcag accagttttt
     1141 tgtcaacgcc gacgtgaatg ggctgaacaa ctcggtgtgg ccgttgttcc tgatgctgac
     1201 ctgccgcaac ggcatgttcg atgacgcggc caagtacacc atttcggaat ccctactccg
     1261 gcgcccgaat cgcggggcca tcgcctgcgc ggcggccagc gggttgtccg tcccggccgc
     1321 ggccaactac ttcgccgatg gctttaccgc gtccctggtc aacacccagc agcaggcccg
     1381 ggtgggagac ctgatgaacg cgggcttcct gaatttgtgg acggtttccc ccaaccagcc
     1441 ggaactgctg ttctacagta tcatcggaga cccggcgcag gtggtgaatc cctgacggac
     1501 gcaaaccatg aacttcggga ggaacaatcg agcatggcgg ctggcggtgg ccggttgcct
     1561 ctttgcctgc cgcgtcctgg ccgacgacca tggcgatgcc gccgttgccg caacagccct
     1621 ggttcccgac ggcggcgcgg tcgccggcgt cattaacgat gacgtcgaca aggactggtt
     1681 ttccttcagc acgctgccgt gggtcggcta ctcgatcacg gtggccacgg gcacgctgtg
     1741 ggacagcgag gcggatctct atgcatccga cggcgctacc tgccagcgcg cgaccaactc
     1801 ggccggatgg gcgacgccgc tgaccgtggc ctggagcaat tccggcgcga ggacgaaacg
     1861 gcacctcgag atcggcggct tcgcggagtt cacgacgggc acgtattcgg tggcggtcgc
     1921 acgtctgaac tgggtggatg ccgatggcga tgggatgttc gatccctggg aagtggagat
     1981 gctcgggagc accaaccagc cggccggtgg cgacccggat aacgacgggt ttccgaatat
     2041 cgacgagtac tacctgcaaa ccgatccgga cgacgcggat tcgggcttgt tcgtggttcg
     2101 gttgaccccg gcggccaccg ggaccgtcgt gcgctgggat gccgcgcaga acgggaccta
     2161 ccgcctgtgg tcgagcacca acctttcgag cgcggcgccg ggttggacct tctttggcga
     2221 gaagacgaag ttcgacgcgt cgccggcctg ggacgaactc gtggatccgg ccgccaccaa
     2281 cgctcgacgc caattctacc gggtcgagtt cacctactga ttggcccctg ttttccgcaa
     2341 tgaaatagac atcggaataa agtatacaat gcctattgaa atagtggtgt atctgttgta
     2401 cttttaagca ggagaatgaa atgaaagcat ttcaaaacat cattgaaacg gtgggccgga
     2461 cgccccttgt gaagctgcag cggctttccg aggagacggg cgcggaaatc ttcatcaaga
     2521 tggaatccca caacccgatg ggcagcgtga aggatcgtat cggcgtggcg atgatcaacg
     2581 aggccgagaa ggccgggctg atcaaggcga acaccgtgat cgtcgagccc acaagcggca
     2641 acacgggcat cgcgctggcc ttcgtcgcgg cggcccggaa gtacaagctc gttctgacga
     2701 tgcccgagtc gatgagcctt gagcggcgcg cgatgctgcg cgccctcggc gcggacctga
     2761 tgctgacccc ggcggagcag ggtatgcggg gcgcgatcgc caaagccagc gagttggtgg
     2821 agaaaacgcc gggcgcctgg atgccgcagc agtttgaaaa cccggccaac cccgctatcc
     2881 atgaggcgac cacgggcccg gagatctggg agtcgagcgg cgggaagatc gatgcgatcg
     2941 tcgccggcgt cgggaccggt ggcacgatca ccggcgtggc gcggttcctc cggaagcaga
     3001 acccggcctt tcaagccttt gccgtggagc cggctgattc gccggtcatc agcggc
//
LOCUS       DAOOTK010000208         2947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_22459_length_2947_cov_5.163209, whole genome shotgun sequence.
ACCESSION   DAOOTK010000208 DAOOTK010000000
VERSION     DAOOTK010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22459_length_2947_cov_5.163209"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..271)
                     /locus_tag="PK634_14300"
                     /pseudo
     CDS             complement(1..271)
                     /locus_tag="PK634_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
     gene            complement(208..843)
                     /locus_tag="PK634_14305"
     CDS             complement(208..843)
                     /locus_tag="PK634_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOW98948.1"
                     /translation="MHIVVCIKQVPGTTKVQINPETNTLIREGVEAIINPFDENALEA
                     ALVLKETGGAKVTVLCMGPPQAETALRESMARGADDIVLLCDRAFAGSDTWATSYALA
                     QGIRTLGAVDLVFCGKQAIDGDTAQVGPGVAEFLGWPVITYVRKLEVKDGKALIERTF
                     EDGYEKIEAPLPCVLTTVKEMNVPRMASLKGRMRAKKACSVMKKNGFCNRR"
     gene            complement(960..2120)
                     /locus_tag="PK634_14310"
     CDS             complement(960..2120)
                     /locus_tag="PK634_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOW98949.1"
                     /translation="MDYGLTEQQQEIRSIMREFAEERVKPVRAELDEKDIFPRELLNE
                     LGKMDLMGLYIPEEYGGFGAESMLDFVIAIEELSRVCIGVSVSYAANALGADPIIIGG
                     SDEQKKKYLAPLAKGEKLAAFALTEPNAGSDAGGIQTTAVKKGDKYVLNGTKQWITNG
                     GEADTYSVMAMTDRSKGARGASFFILEKGMPGFTFGKKENKMGIRASATRELIFEDCE
                     VPAANLIGREGMGFIIAMKTFDVSRPGIAAQGVGLAQGALDEVINYARQRVQFGKPII
                     ANQGYQWTLADLATKTEAARALLYSVCRTADKSTKDISKYSAMIKLFATDVAMDVTTW
                     AVQLLGGYGYMKEYPVEKMMRDAKILQIYEGTNQVQRDVIGAALIKEYASKK"
     gene            complement(2314..>2947)
                     /locus_tag="PK634_14315"
     CDS             complement(2314..>2947)
                     /locus_tag="PK634_14315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOW98950.1"
                     /translation="MLAAMLFFGLGALFQPGSRMLVAMGALVPALFQEGEGWRLITYG
                     YLHIGLMHIAFNMIALSQVGPVLEEQVGRARFFVVYTLAVIGGGLADILFRDPISIVA
                     GASGALFGLIGFGMSYAHFYGGFAGRMQRDFFLRWAVYGFVFGLLVGADNIAHAGGLV
                     TGALLGFLVERERVRGERFDPLWNALAGLCAAATVGAFVWMAVAGVGAAG"
BASE COUNT          476 a         1024 c          921 g          526 t
ORIGIN      
        1 ccgtgccgtg atcgggcgag gtgcggatga tcggcaggcc gagcgtgatg ttcacgccac
       61 tgtcaaaagc cagcatctta aagggaatgc caccctgatc atgatacatg caaatgtggc
      121 agtcaaaacg cttccgcgcc tgcggtgtaa aggccgtatc cggcggcagt ggtccttcga
      181 tcgtgaagcc gcgcttgcgc aaacgtgcta gcgtcggttg caaaatccgt tcttcttcat
      241 caccgaacaa gccttcttcg cccgcatgcg gcccttgagc gaggccatgc gcgggacgtt
      301 catttccttg accgtggtga gcacgcacgg cagcggcgcc tcgatcttct cgtagccgtc
      361 ctcgaacgtc cgctcgatca gcgccttgcc gtccttgacc tccagcttgc gcacgtacgt
      421 gatcaccggc cagccgagga actcggcgac gcccggcccg acctgcgccg tgtcgccgtc
      481 gatcgcctgc ttgccgcaga agaccaggtc cacggcgccc agcgtgcgga tgccctgggc
      541 cagcgcgtag gaggtcgccc acgtgtccga gccggcgaag gcgcggtcgc agagcagcac
      601 gatgtcgtcc gcgccgcggg ccatggactc gcgcagcgcc gtctcggcct gcggcgggcc
      661 catgcacaac acggtcacct tcgcgccgcc cgtttccttg aggacgaggg cggcctcgag
      721 ggcgttctcg tcgaagggat tgatgatcgc ctcgacgccc tcgcggatca gcgtgttcgt
      781 ctccgggttg atctgcacct tcgtggtgcc cggaacctgc ttgatgcata cgacgatgtg
      841 catacgttct cccgcgcggt gaggtagggc gcgtccgctg aacgcgccgc ccgggttttt
      901 gcacgaagcg gcgcccgggc gcgcggcggc ttgagccaag ccgccctacc gacggcttcc
      961 tacttcttcg acgcgtactc cttgatcagc gccgcgccga tcacgtcgcg ctggacctgg
     1021 ttggtgccct cgtagatctg caggatcttg gcgtcccgca tcatcttctc gacggggtat
     1081 tccttcatgt acccgtagcc gccgaggagc tgcacggccc acgtggtgac gtccatggcc
     1141 acgtcggtcg cgaagagctt gatcatggcc gagtacttcg agatgtcctt ggtgctcttg
     1201 tccgccgtcc ggcagaccga gtagagcagc gcgcgggcgg cctcggtctt cgtcgccagg
     1261 tccgccagcg tccactggta gccctggttt gcgatgatgg gcttgccgaa ctggacgcgc
     1321 tggcgcgcgt agttgatcac ctcgtccagc gcgccctgcg ccaggcccac gccctgcgcc
     1381 gcgatgcccg gccgcgacac gtcgaacgtc ttcatggcga tgatgaaccc catgccctcg
     1441 cggccgatga ggttcgccgc cggcacctcg caatcctcga agatcagttc ccgcgtcgcc
     1501 gaggcgcgga tgcccatctt gttttccttc ttgccgaacg tgaagcccgg catgcccttc
     1561 tccaagatga agaacgaggc gccgcgcgcg cccttgctcc ggtcggtcat ggccatcacc
     1621 gagtaggtat ccgcctcgcc gccgttggtg atccactgct tcgtgccgtt gaggacgtac
     1681 ttgtcgccct tcttcacggc cgtcgtctgg atgccgccgg cgtcgctgcc cgcgttgggc
     1741 tcggtcaggg cgaaggcggc cagcttttcg cccttcgcca ggggggcgag gtatttcttc
     1801 ttctgctcgt ccgagccgcc gatgatgatc gggtccgcgc cgagggcgtt ggcggcatag
     1861 gagacggaca cgccgatgca gacgcggctc aactcctcga tggcgatgac gaaatccagc
     1921 atgctctccg cgccgaaccc gccgtattcc tcggggatgt acaggcccat caggtccatc
     1981 ttgcccaatt cgttgagcag ttcgcgcggg aagatatcct tctcatccag ttcggcgcga
     2041 acgggtttca cccgctcttc cgcgaactcg cgcatgatgc tccgaatttc ctgttgctgt
     2101 tcggtcagcc cataatccat ttccgccatg atatccttcc tctccgggcg tttgcgtcgc
     2161 cggtggcctg ctcccggtcc atccgggcgc ggcacagtaa atacggttcg gggccgggaa
     2221 tcaagaggtt tcccgtcttc gccaaggcca gggcgggcag gcggggttca ggggacagga
     2281 agccggatcc gccttctatt caccattcac tgctcacccc gcagccccca cccccgccac
     2341 ggccatccac acaaacgcgc cgacggtcgc ggcggcgcag aggccggcga gggcgttcca
     2401 gagcggatcg aaccgctccc cgcggacccg ctcgcgctcg accaggaagc ccagtagcgc
     2461 gccggtgacc aggccgccgg cgtgggcgat gttgtccgcc cccaccagca ggccgaacac
     2521 gaagccgtac accgcccaac ggaggaagaa atcgcgctgc atccggcccg cgaacccgcc
     2581 gtagaagtgc gcgtagctca tgccgaaccc gatcaggccg aagagcgcgc ccgaggcgcc
     2641 ggcaacgatc gagatcggat cgcggaacag gatatccgcc agcccgccgc cgatcaccgc
     2701 gagcgtgtag accacgaaga accgcgcccg gccgacctgc tcctccagga cgggcccgac
     2761 ctgcgagagg gcgatcatgt tgaaggcgat gtgcatcagc ccgatgtgga ggtagccgta
     2821 cgtgatcagc cgccatccct cgccttcctg gaacagcgcg ggcaccaggg cgcccatcgc
     2881 gaccagcatg cgcgagcccg gctggaagag cgcgcccagg ccgaaaaaca gcatcgccgc
     2941 cagcatc
//
LOCUS       DAOOTK010000209         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_23597_length_2856_cov_4.263120, whole genome shotgun sequence.
ACCESSION   DAOOTK010000209 DAOOTK010000000
VERSION     DAOOTK010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23597_length_2856_cov_4.263120"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PK634_14320"
     CDS             complement(<1..524)
                     /locus_tag="PK634_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOW98951.1"
                     /translation="MGAIRRAGLALLAGGMSARAVTDPLPGETSMTGKTESVITLPSP
                     VAERHCELNHALQFRRSTREFKDEPISLAEAGRLLWAAQGVTSLGGFRTAPSAGALYP
                     LELYLVAGRVDDLKAGIYKYDATKHSLVRTAEGDRRADLAAASLGQQWMAQAAAILAF
                     SAVPERTTRRYGERG"
     gene            1016..1798
                     /locus_tag="PK634_14325"
     CDS             1016..1798
                     /locus_tag="PK634_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HOW98952.1"
                     /translation="MKRNGLQKWMLMAGLVMTAAAGAWGAEASLDLSLNSHYIWRGQV
                     LNDEPVFQPSLTVSGPCGLSFNTWANMDVTDNLDNQGEFNEVDLTVSYSHNLCEKVSL
                     EVGIVDYVLKTAENTGELYLVAGADVLLSPTVRLYYDFDEVQDFYAQLAVGHSMAFLE
                     EKLSLDLGASLGFGGSDYNEYYFTFTPAEGEEGEAIVNDSAALNDLNLSAGLTYAFSE
                     TLSLAGNVQYVALLDSDIEDAAEAVYGEKDAVYGGVTLSRSF"
     gene            complement(1907..2551)
                     /locus_tag="PK634_14330"
     CDS             complement(1907..2551)
                     /locus_tag="PK634_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOW98953.1"
                     /translation="MDPRGHESPEPRWLLWARELQALAQNGLHYATGDFDRQRYEAVQ
                     RIAAEMFAVQSGADTRRILDLFRQEIGHATPKVDMRGAVFRDGRILLVREKRDQLWTL
                     PGGWADPNELPTEAIEREIREEAGYRTRAVKLAAVYDRCRQGAIPPYPYHVYKIFFVC
                     ELIDGSPVENIETDAAEFFAEGEIPALSIPRVNPRQIACLFQHHRNPGLPADFD"
     gene            2648..>2856
                     /locus_tag="PK634_14335"
     CDS             2648..>2856
                     /locus_tag="PK634_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98954.1"
                     /translation="MEIFFEYRPSTDTCHGHVRRAQGLGLIPAVLSLAMMLAVRTGPG
                     PGLWSALGSVLGLQLVLWGASRLPSV"
BASE COUNT          463 a          956 c          951 g          486 t
ORIGIN      
        1 ccccgctccc cgtatcgccg ggtggtccgt tcgggcacgg cgctgaacgc caggatcgcg
       61 gcagcctggg ccatccattg ctggccaagg gaggcggcgg ccaggtcggc ccgccggtcg
      121 ccctccgccg tccggaccag cgagtgtttc gtcgcatcgt acttgtaaat gccggccttc
      181 aggtcgtcca cgcggcccgc gaccaggtag agttcgagcg ggtagagcgc gcccgccgac
      241 ggcgccgtgc ggaacccgcc gaggctcgtg acgccctggg ccgcccagag cagccggccg
      301 gcctccgcca gggagatggg ctcgtccttg aattcacgcg tcgagcggcg gaactgcagg
      361 gcatggttca gttcgcaatg gcgctcggcc accggcgagg gcagcgtgat cacggattcc
      421 gtcttgcctg tcattgaagt ttctcctgga agcgggtccg tcacggcgcg cgccgacatc
      481 ccgcccgcca gcagcgcgag gcccgccctc cgaatcgctc ccattcccat atccttctcc
      541 cggggccccg ccggccggaa gaggcgccac ccggctccac ctccgcgcgc cagcctagaa
      601 aagcccgccc tgcttgacca gcctcttttt tcatgccgac cggccgcccc gccgatccgc
      661 gcttgcgcgc cgaccgcggc gcgccgcaca cgggcccggc tcccaactgc ctgctggcaa
      721 gaaagacatt attgtcattg aattacactt ctgcatgtaa taaaggcgtt tcaaacatat
      781 cggtacattt ccgccatcta cgcatcttat tataaattaa ttggttatgt aatgtgccaa
      841 gccgcatgcc gtccccgtgg cacgaagagt gcatggggtt ggtgtcgaac cgacaggcgg
      901 cgcgtccggg atggatttct ttgacatgcc gcggccggac gggaaaaatg cagcgaaacc
      961 gccgctgtct ccgccaaccc ggcggggtcg gcggccaaca ggagaacgat acatcatgaa
     1021 gagaaacgga ctgcaaaagt ggatgctgat ggcgggcttg gtcatgaccg ccgcggccgg
     1081 ggcctggggc gcggaagcca gcctggacct ctcgcttaat tcgcactaca tctggcgcgg
     1141 ccaggtcctg aacgacgaac ccgtcttcca gccctcgctg accgtcagcg ggccgtgcgg
     1201 gttgtccttc aacacatggg cgaacatgga cgtgacggat aacctggaca accagggcga
     1261 gttcaacgag gtggacctga ccgtgtcgta cagccacaac ctgtgcgaga aggtcagcct
     1321 cgaggtcggc atcgtggatt acgtgctgaa gaccgcggag aacaccggcg agctgtacct
     1381 ggtcgccggc gccgacgtcc tgctgtcgcc gaccgtcagg ctgtattacg acttcgacga
     1441 ggtccaggac ttctacgcgc aactggccgt cggccacagc atggccttcc tggaggagaa
     1501 gctctccctg gacctggggg cctccctggg cttcggcggc tcggactaca acgagtatta
     1561 cttcaccttc acccccgccg agggcgagga gggcgaggcc atcgtcaacg acagcgccgc
     1621 gttgaacgac ctgaacctgt ccgcgggcct gacctacgcg ttctcggaga cgctctccct
     1681 ggccggcaac gtgcagtacg tggcgctgct ggatagcgac atcgaggacg ccgcggaagc
     1741 cgtctacggc gagaaggacg ccgtgtacgg cggcgtgacc ttgagccgct cgttctgacg
     1801 cccattcgca agggaggcga ccgcggatcg cacggatgac gcggacagga ctggctccag
     1861 ccttttccat ccgcgccatc cgcggttcta cattccgcct tcgggttcaa tcgaagtcgg
     1921 cgggaaggcc cggattccgg tggtgctgaa aaaggcaggc gatctgccgc gggttgacgc
     1981 gggggatgga gagcgccggg atttctccct ccgcgaagaa ttcggccgca tccgtttcga
     2041 tgttctccac cggcgaaccg tcgatcagct cgcagacgaa gaatatcttg tacacgtggt
     2101 acgggtacgg cgggatggcg ccctggcggc agcggtcgta gacggcggcc agcttgacgg
     2161 cgcgggtgcg gtagcccgcc tcctcgcgga tctcgcgctc gatggcctcg gtcggcagtt
     2221 cgttgggatc ggcccagccg cccggcagcg tccagagctg gtcgcgtttc tcgcggacga
     2281 gcaggatccg gccgtcgcgg aacaccgcgc cgcgcatgtc caccttgggg gtggcgtggc
     2341 cgatctcctg ccggaacagg tcgaggatgc gccgggtgtc ggcgccggac tgcacggcga
     2401 acatctcggc ggcgatgcgc tggacggcct cgtagcgctg ccggtcgaaa tcccccgtgg
     2461 cgtagtgcaa cccgttctgc gcgagcgcct gcagctcgcg cgcccacagc agccagcggg
     2521 gctccgggga ttcgtggcct cgcggatcca tggccggact atagcacagg gggccggccc
     2581 cgcctaccct gcgcgacggc gcgcccgcgg ttgacagggc cacgccggcg ggcgataagg
     2641 agggcggatg gaaatctttt tcgaataccg tccttccacg gatacctgcc acgggcatgt
     2701 gcggcgcgcc caggggctcg gcctgatccc tgccgtcttg tccctggcca tgatgctggc
     2761 ggtgcggacg gggcccggcc cgggcctgtg gagcgccctg ggctcggtgc tcggcctgca
     2821 actggtgctc tggggcgcct cccgccttcc ctcggt
//
LOCUS       DAOOTK010000210         2819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_24065_length_2819_cov_5.421491, whole genome shotgun sequence.
ACCESSION   DAOOTK010000210 DAOOTK010000000
VERSION     DAOOTK010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24065_length_2819_cov_5.421491"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2819
                     /locus_tag="PK634_14340"
     CDS             <1..>2819
                     /locus_tag="PK634_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOW98955.1"
                     /translation="DVSLDRPVRPGPALRVSGDPAASVSWGDAVNVVDGRDQEVYGRD
                     AFYPVSPATIEGFSQMRKWKMVRVRVSPAQYNPVQGTLRIVEHLDFQIRFARGRATQS
                     AALGDAVMDAAALGLVENDDAARGWYATRTKTATSSGNTYLIMTTDEIYTASPTLYGL
                     TTHKEDMGFTVHVVTETKLDGSAAATGWNEVTGQYPDGKADRMRKWLQNNYIGMQIEY
                     VLLVGNPAPDWGDLPMKNCSYQAYVYPADGYYADLTGNWDIDGNGIYGNETNDVGSVG
                     GVDRVPEVYVGRIPVYTADPSWSEMLRRIVWKSIKYEQEGYTGWRQSALLPESWSDSY
                     TDGAYLGEYARTNYLSSKGYSAHTLFEQGSVNPIYDSSFPSDEELLDDSTARRWMNHD
                     YGMVLWWGHGWSRGASVYAGGTLYESDQCPMLDDEHPASVFMVSCSCGDPVDDQNLGY
                     SMLRNGGIASVAAANVSWYYYCSWSPADSKGMNASMGYDFFRKVVSNETTYGRALAEV
                     KEEMDDWWNNFYTFNLYGDPSLRITSQGVDSDGDTMADGWEADHGLDAGDPDDAGLNP
                     DGDSYSNLEEYRAGLDPQTIDSPASTHSSIAVPGTFNEWSTTAHPLRKIGGGAWQADI
                     VLTNQTGVLFKFAANGSWTTNWGEYNQLATNAPFSGLADWESDNIVVAGTLDGSYRFT
                     FNELTRGYRLEPTPAPDADLDGMPDAWETAHELNPHNGADAAEDPDYDGYTNLQEYGN
                     GTDPHVWNPRAGNYNYVSVPGTFNGWNAAASNMCLVADHVWRYDATFSGESAIEFKFA
                     ANGSWAVNWGDTNQTQFTPPMAGGGESGAANLIISGSLSGRYRFTFNDQSLAWGVEAI
                     ATPDTDGDGMSDAWENARGLNYRSAADAGADADGDGLCNREECDFGSEPGDRDSDDDG
                     AEDEAEFIAGTDPMDEESLFSAETA"
BASE COUNT          528 a          927 c          944 g          420 t
ORIGIN      
        1 gcgacgtttc gctggatcgc ccggtccgcc ccgggccggc cctgcgcgtg tcgggggatc
       61 ctgcggcgtc cgtgtcctgg ggcgacgcgg tgaatgtcgt ggacggcagg gaccaggagg
      121 tctatggccg cgacgctttc tatcccgtat cgcccgcgac gatcgaaggc ttttcgcaga
      181 tgcgcaagtg gaagatggtc cgggtgcgcg tctcgcccgc ccagtacaac ccggtgcagg
      241 gaaccctccg catcgtcgaa catctcgatt tccaaatccg ttttgcgcgc ggccgcgcca
      301 cgcagtccgc agccctgggc gacgcggtga tggatgccgc cgcgctgggc cttgtcgaga
      361 acgacgatgc agcccggggc tggtatgcga cacgcacgaa gaccgcgacc tcgtccggca
      421 atacctacct gatcatgacg accgacgaga tctacacggc cagcccgacc ctctatggcc
      481 tgacgacgca caaggaagac atgggcttca ccgtgcacgt ggtcacggaa acgaaactcg
      541 acggcagcgc cgccgcgacc ggctggaacg aggtcaccgg ccagtatccg gacggcaagg
      601 ccgaccggat gcgcaagtgg ctgcagaaca attatatcgg catgcagatc gagtatgttc
      661 tcctggtcgg aaacccggcg ccggactggg gcgacctgcc gatgaagaac tgctcctacc
      721 aggcctacgt ctatccggcg gacggctact acgccgatct cacgggcaac tgggacatcg
      781 acggcaacgg gatctacggg aacgaaacca acgatgtggg ttcggtgggc ggcgtggacc
      841 gggtgccgga agtgtacgtc ggccgcatcc ccgtctacac cgccgatccc tcctggagcg
      901 agatgctgcg gcggatcgtc tggaagtcga tcaagtacga gcaggaaggc tatacgggct
      961 ggcgccagtc cgcgctgctc ccggagagct ggtcggacag ctacaccgac ggcgcgtacc
     1021 tcggcgagta cgcgcggacc aactacctgt cctcgaaggg ctacagcgcg cacacgctct
     1081 tcgagcaggg cagcgtcaat cccatctacg attccagttt cccgtccgac gaggaactgc
     1141 tcgacgacag cacggcccgg cgctggatga atcacgacta cggcatggtc ctctggtggg
     1201 gccacggctg gtcgcgcggc gcctcggtct atgccggcgg gacgctctac gagagcgacc
     1261 agtgcccgat gctcgacgac gagcatccgg cctccgtgtt catggtgtcc tgctcctgcg
     1321 gcgacccggt cgacgaccag aacctgggct actcgatgct gcgcaacggc ggcatcgcgt
     1381 ccgtcgccgc cgccaacgtg tcatggtact actattgctc ctggtcgcct gcggattcca
     1441 agggcatgaa cgcgtccatg ggctacgact tcttccgcaa ggtggtcagc aacgagacca
     1501 cgtacggccg tgccctggcc gaggtgaagg aggagatgga cgactggtgg aacaacttct
     1561 acaccttcaa cctgtacggc gatccgtccc tgcgcatcac gtcgcaaggc gttgactcgg
     1621 atggcgatac gatggcggac ggctgggaag ccgaccacgg gctggacgcc ggcgatccgg
     1681 acgacgcggg cctgaacccc gacggcgaca gctactcgaa cctggaggag taccgggccg
     1741 gtctcgatcc gcagacgatc gattcgcccg cctccacgca cagttccatc gccgtgccgg
     1801 ggacgttcaa cgagtggagc accacggcgc acccgcttcg caagatcggc ggcggcgcct
     1861 ggcaggccga catcgtcctc accaaccaga ccggcgtgct gttcaagttc gccgcgaacg
     1921 gttcgtggac gacgaactgg ggcgagtaca accagctcgc cacgaacgcg ccgttcagcg
     1981 ggctggccga ctgggaatcg gacaacatcg ttgtggcggg gacgctcgac ggcagctacc
     2041 ggttcacgtt taacgagctc acccgcggct accgtctcga gccgacgccc gcgccggacg
     2101 cggacctgga cggcatgccc gatgcctggg agacggcgca cgagttgaat ccgcacaacg
     2161 gcgcggacgc cgcggaagac ccggactacg acggctacac gaacctccag gagtacggaa
     2221 acggcaccga tccccatgtc tggaatccgc gcgcgggcaa ctacaactac gtctcggtcc
     2281 ccggcacgtt caacggctgg aacgcggcgg cgtccaacat gtgcctggtg gcggaccacg
     2341 tatggcgcta cgacgccacg ttctccggcg agagcgccat cgaattcaag ttcgcggcca
     2401 acgggagctg ggcggtcaac tggggcgaca cgaaccagac ccagttcacc ccgcccatgg
     2461 ccggtggcgg ggagagcggg gccgccaacc tcatcatcag cggttcgcta agcggccgct
     2521 accggttcac gttcaacgac cagtcgctgg cctggggcgt ggaggccatc gcgacgccgg
     2581 acaccgacgg cgacgggatg tcggacgcgt gggaaaacgc gcgcggcctg aactaccggt
     2641 cggccgcgga tgctggcgcg gacgccgacg gcgacggcct gtgcaaccgc gaggagtgcg
     2701 acttcgggag cgagccgggc gaccgggaca gcgacgacga cggggcggaa gacgaggccg
     2761 agttcattgc ggggaccgat ccgatggacg aggagtcgtt gttcagcgcc gagaccgcc
//
LOCUS       DAOOTK010000211         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_30096_length_2448_cov_3.987046, whole genome shotgun sequence.
ACCESSION   DAOOTK010000211 DAOOTK010000000
VERSION     DAOOTK010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30096_length_2448_cov_3.987046"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PK634_14345"
     CDS             <1..857
                     /locus_tag="PK634_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOW98956.1"
                     /translation="ANGHILLEGVPGLAKTLSVKTLAAALDMSFQRIQFTPDLLPADL
                     VGTLVYNPKEGSFATKKGPVFAHIILADEINRAPAKVQSALLEAMQERQVTIGQDTFP
                     LPKPFLVLATENPIEQEGTYPLPEAQVDRFMLKLRITYPSIEEERRILDAMAVTNKQF
                     DVRPAVKPEAILQAQQVVDDIYVDDKIKDYILSIVFATREPEKYKLALKEFIRYGASP
                     RATIYLTVAARAHAFLSGRGYVTPQDVKSVAPDVLRHRVIVSYEAEAEEMNSDDVIAK
                     ILNELPVP"
     gene            988..1872
                     /locus_tag="PK634_14350"
     CDS             988..1872
                     /locus_tag="PK634_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOW98957.1"
                     /translation="MIPKEIIKKVRQIQIRTRHKVSDVFAGEYHSVFKGRGMEFQEVR
                     EYVPGDDIRSIDWNVTARMGHPFVKKFTEERELTVMLLLDISASNLFGSTAQLKKDLA
                     AELAAVLAFAAIQNHDRVGLVLFSDEVEGYVAPRKGVSHVLRVIREALYFQPKRRGTR
                     LVPALDFLNHVMARKTVSFLISDFVSDEDLKPALSVTARRHDLIALLVGDRRERAWPS
                     AGLVEWRDAETGRVYLADTSSSAVRRSFAELQQDRRKQALSLLRGCGVDAVEVHAGEP
                     YERELMKFFQTRERRFRT"
     gene            1869..>2448
                     /locus_tag="PK634_14355"
     CDS             1869..>2448
                     /locus_tag="PK634_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98958.1"
                     /translation="MRRNSRVGLSGLLLFAAVAGPSCGPRSRPAKSDLPDFAVQAALS
                     TNVLRVGDVAELTLTAAHPAGSRWEPPRVDREAEIVVREQRQGSEPLADGRVLSRATY
                     RLTSLALGEHVVSTGEVAFVLADGTKHVEPFPGVRFRVDTVLTNENAELRGIKGPSRW
                     PAPFPWWAAALAAVALVALALGLVTARALRRPR"
BASE COUNT          414 a          836 c          806 g          392 t
ORIGIN      
        1 tggccaacgg gcatatcctg ctggagggcg tgccgggcct ggccaagacg ctctccgtga
       61 agaccctggc tgccgcgctg gacatgtcgt tccaacgtat ccagtttacg cccgacctcc
      121 tccccgccga tctggtcggc acgctggtgt acaatcccaa ggaaggctcc ttcgccacga
      181 agaagggccc ggtcttcgcc catatcatcc tggccgacga aatcaaccgc gcgccggcca
      241 aggtgcagag cgcgctgctg gaggccatgc aggagcgcca ggtgacgatc ggccaggata
      301 ccttcccgct gccgaagccc ttcctggtgc tcgcaacgga gaatcccatc gagcaggaag
      361 gcacctatcc cctgcccgag gcgcaggtgg accggttcat gctgaagctg cggatcacct
      421 acccctccat cgaggaggag cgccgcatcc tcgacgccat ggccgtgacc aacaagcagt
      481 tcgacgttcg tcccgccgtg aagcccgagg cgatcctcca ggcgcagcag gtcgtggacg
      541 acatctacgt ggacgacaag atcaaggact acatcctgag catcgtcttc gccacgcgcg
      601 agccggaaaa gtacaagctc gcgttgaagg agttcatccg ctacggcgcg tcgccgcgcg
      661 cgacgatcta cctgaccgtc gccgcccgcg cccacgcctt cctctcgggc cggggttacg
      721 tgacgccgca ggacgtgaaa tccgtggcgc cggacgtcct gcgccaccgc gtcatcgtgt
      781 cctacgaggc cgaggccgag gagatgaact cggacgacgt gatcgcgaaa atattaaatg
      841 aactaccggt gccgtgatcc ggaacatgaa ccacagagga cacagagggc gcggaggcga
      901 ccgctgggct ttgcggagca aggactttcg tttccctctg cggcctctgc gacctccgtg
      961 gttcattttt tttgaagcga tgccgccatg atcccgaaag agatcatcaa gaaggtccgg
     1021 cagatccaga tccggacgcg ccacaaagtc tccgacgtgt tcgccgggga ataccacagc
     1081 gtgttcaagg gccggggcat ggagttccag gaggtccgcg agtacgtgcc gggcgacgac
     1141 atccgctcga tcgactggaa cgtcacggcg cgcatgggcc atccgttcgt caagaaattc
     1201 acggaggagc gcgagctgac cgtcatgctg ctgctcgaca tcagcgcgtc gaacctcttc
     1261 ggcagcacgg cgcagctcaa gaaggacctg gccgccgagt tggccgccgt gctcgcgttc
     1321 gcggcgatcc agaaccacga ccgggtcggg ctggtcctgt tctccgacga ggtcgagggg
     1381 tacgtcgcac cgcgcaaggg cgtctcgcac gtgctgcggg tcatccgcga ggcgctgtac
     1441 ttccagccga aacgccgcgg cacgcgcctg gttcccgccc tcgatttcct caaccacgtc
     1501 atggcccgca agacggtctc gttcctcatc agcgacttcg tgagcgacga ggatctcaag
     1561 ccggcgctgt cggtcacggc gcggcggcat gatctcatcg cgctgctcgt cggcgaccgt
     1621 cgcgagcggg cctggccctc ggccggcctc gtggaatggc gggatgccga gacgggccgg
     1681 gtgtacctcg cggatacgtc gtcctcggcc gtccggcgga gctttgcgga actccaacag
     1741 gaccgccgga agcaggcatt gtcgctgctg cgcggctgcg gcgtcgacgc cgtggaggtg
     1801 cacgcgggcg agccatacga gcgggaactg atgaagtttt tccaaacgag ggagcggcga
     1861 ttccggacat gagaaggaat tcccgagtcg gcctttccgg gctgctgctg ttcgcggcag
     1921 tggccggccc gtcctgcggg ccccgctccc ggcccgcgaa atcggacctg cccgatttcg
     1981 ccgtccaggc cgccttgagc accaatgtcc tgcgcgtcgg ggacgtcgcg gaactgaccc
     2041 tgaccgcggc gcaccccgcc gggagccgat gggaaccgcc gcgcgtggac cgggaggcgg
     2101 agatcgtggt ccgcgagcag cggcagggca gcgagccgct ggccgacggc cgcgtcttga
     2161 gccgcgcgac ataccgcctg acctccctgg cgctcggcga gcacgtcgtg tccaccggcg
     2221 aggtggcctt cgtgctggcc gacggcacga aacacgtgga gccgtttccc ggcgtgcggt
     2281 tccgcgtgga caccgtcctg acgaacgaaa acgccgaact gcgcggcatc aagggcccgt
     2341 cccgctggcc cgcccctttc ccgtggtggg ccgccgccct cgccgccgtg gcgctggtcg
     2401 ccctggccct gggcctggtc accgcgcggg ccctccgccg gccgcgta
//
LOCUS       DAOOTK010000212         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_31710_length_2368_cov_4.341980, whole genome shotgun sequence.
ACCESSION   DAOOTK010000212 DAOOTK010000000
VERSION     DAOOTK010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31710_length_2368_cov_4.341980"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK634_14360"
     CDS             <1..390
                     /locus_tag="PK634_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOW98959.1"
                     /translation="APAARPDVLLVDTTGELMNFYSCATIIFVGKSLSEQKGGQNVIE
                     PAWWGKPVLVGPNMKNFAAVMDEFLEASAILQVPDAHGLKMAILGLLDDPVRRVELGA
                     RAARLVREKAGAGARTLDRIGPVIFPP"
     gene            complement(391..1359)
                     /gene="lgt"
                     /locus_tag="PK634_14365"
     CDS             complement(391..1359)
                     /gene="lgt"
                     /locus_tag="PK634_14365"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOW98960.1"
                     /translation="MNWIGPYIHRIDPVLGTAGGLYLWWYGASFSLGFLNAHLYIRRK
                     RRALGLSLGAVYTLSLFLAIGVLLGGRTVEVLFYEWPFYSAHPHLIPAVWLGGMATHG
                     LLFGGLAGVWLFCRRRRLSFLRVTDVLAIPAAFILAMGRIGNFIDGQIVGRVTDAWWA
                     VRFPDAEGFRHPVVLYDGLKNLLLIPVLLRASARRLRPGAVTGLFLLLYAGLRVPIDV
                     FREYPTTLLGLATGQSLNLLMATGGLILLILRWRREPEAPPFTPGIVVRPERLVARPL
                     LFALLIALAPIIPSDWTQDIPARYGKRHPGMKHSALYPVLDTAPPD"
     gene            1444..>2368
                     /locus_tag="PK634_14370"
     CDS             1444..>2368
                     /locus_tag="PK634_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOW98961.1"
                     /translation="MRSASRRCSVPSVSSPITRRRFIEKLGAGTLATLALWHELRAES
                     AVPAAPPPAPGTPSDDLLAAEEAYFADFAKVFTIADEHKYLVASQKGSMPIPILRHFK
                     EGLDRIARDPFPVYLEPSAVTRAKIAKGYGARVDEIAISRNTTDAISQILHGLDWRAG
                     DEILCSTLEYPNCVATVRRVAGRFGLAIRQFGVPSRPDFSAEEIVESARRRIAPGKTK
                     AMFFSCPAHPSGVAMPVRRLARLAQEHGITTVVDGAHYGGMFVPRLDETGIDFWGISG
                     HKWQCGPGGTGILYARNARWPANPNPLPRFHI"
BASE COUNT          401 a          817 c          808 g          342 t
ORIGIN      
        1 gcgccggccg cgcggccgga cgtcctgctg gtggatacca ccggcgagct gatgaacttc
       61 tattcctgcg ccacgatcat cttcgtgggc aagagcctgt ccgaacagaa gggcgggcag
      121 aacgtcatcg agcccgcgtg gtggggcaag cccgtcctcg tggggccgaa catgaagaac
      181 ttcgccgcgg tcatggacga gttcctcgag gcgagcgcga tcctccaggt gcccgacgcc
      241 cacggcctga aaatggccat cctggggctg ctggacgatc ccgtccgccg ggtcgaactc
      301 ggcgcgcgcg cggcgcgcct ggtccgcgag aaggccggcg ccggcgcgcg cacgctggac
      361 cggatcggtc cggtaatctt tcccccctga tcaatccggc ggcgccgtgt ctaggacggg
      421 gtaaagggcc gagtgcttca tgcccgggtg ccgcttgccg tagcgcgcgg ggatgtcctg
      481 ggtccaatcg ctcgggatga tcggcgcgag ggcgatcagc aaggcgaaaa gcagggggcg
      541 cgccaccagc cgttcgggtc ggacgacgat ccccggagtg aacggcggcg cctccggttc
      601 gcggcgccat cgcaggatca gcaagatcag tcccccggtc gccatgagga ggttcaggct
      661 ctggcccgtc gccaagccga gcagggtggt ggggtactcg cggaagacgt cgatcggcac
      721 gcgcagcccg gcgtagagca gcaggaacag gccggtgacc gcgccggggc gcaggcggcg
      781 ggcggacgcg cggagcagta ccgggatgag cagcaggttc ttcagcccgt cgtacagcac
      841 cacgggatgc cggaatccct cggcatccgg gaaccgtacc gcccaccagg catccgtcac
      901 gcgcccgacg atctggccgt cgatgaaatt gccgatccgg cccatggcca ggatgaacgc
      961 cgccgggatc gccagcacgt ccgtgacgcg caggaacgac agccgccgcc ggcggcagaa
     1021 gagccagacg ccggccagcc cgccgaacag cagcccgtgc gtggccatcc cgcccagcca
     1081 caccgccggg atcaggtgcg gatgagcgct gtagaacggc cattcgtaga aaagcacctc
     1141 gacggtccgc ccgcccaaga gcacgcctat cgcgaggaag aggctcaagg tgtacaccgc
     1201 ccccagtgaa aggcccagcg cccggcgctt tcgccggatg tagaggtggg cgttcaggaa
     1261 gcccagggag aaactcgccc cgtaccacca gaggtatagc ccgccggccg tccccaggac
     1321 gggatcaatc cggtgaatgt aagggccgat ccagttcacg cgcgctccgg cttcaattcc
     1381 acgaggatag ggattatcat gcgggcttgg aaacagattc tgcaacccgg gaggacggtc
     1441 ctcatgcgta gcgcttcacg ccgttgttcc gttccatccg tttcgagccc catcacgcgg
     1501 cgccggttca tcgagaaact gggcgccggc acgttggcca cgctggcgct gtggcatgaa
     1561 ctccgcgccg agtccgccgt gcccgcggct ccgccgccgg cgcccggcac cccgtccgat
     1621 gaccttctgg ccgcggagga ggcgtacttc gccgatttcg cgaaggtctt caccatcgcc
     1681 gacgagcaca agtacctggt cgccagccag aaggggtcca tgcccatccc gatcctgcgg
     1741 catttcaagg aagggctgga ccggatcgcg cgcgacccgt tccccgttta cctggagccc
     1801 tcggccgtca cgcgcgcgaa gatcgcgaaa ggctacggcg cgcgcgtgga cgagatcgcg
     1861 atctcgcgca acacgaccga cgcgatcagc cagatccttc acgggctgga ctggcgggcg
     1921 ggtgacgaga tcctgtgctc cacgctcgag taccccaact gcgtcgccac ggtccggcgc
     1981 gtggccgggc gcttcgggct ggccatccgc cagttcggcg tgccgtcgcg gccggacttt
     2041 tccgcggagg agatcgtgga atccgcgcgc cgccggatcg cgccggggaa gacgaaggcg
     2101 atgttcttct cgtgcccggc ccatcccagc ggcgtggcca tgcccgtccg ccggctggcc
     2161 cggctcgcgc aggagcacgg gatcaccacg gtcgtggacg gcgcgcacta tggcggcatg
     2221 ttcgtcccgc gcctcgacga gaccggcatc gatttctggg gcatctcggg ccataagtgg
     2281 caatgcggcc ccggcggcac cgggatccta tacgcccgca acgcccggtg gcccgcgaat
     2341 ccgaacccgc tcccgcgctt tcatattg
//
LOCUS       DAOOTK010000213         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_33561_length_2289_cov_5.313787, whole genome shotgun sequence.
ACCESSION   DAOOTK010000213 DAOOTK010000000
VERSION     DAOOTK010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33561_length_2289_cov_5.313787"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PK634_14375"
     CDS             <1..594
                     /locus_tag="PK634_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOW98962.1"
                     /translation="ENEPRDKVPNGVISRWPITASGEWTDRVVQNRDFVWATIELPGP
                     RDLHVVSVHLKSGESWYQKKGRQREAVALLDYIRRAGWPADDYLVIAGDLNTVSRNEP
                     LFDIWASLVSDARQPADQQGDKDTNIPRQQCYDYVLPGPALDALHVPVEAGGLTFPDG
                     LVFDSRLWSPPPAPIRPGDSAEEDLQHLPVMKAFRLP"
     gene            749..1174
                     /gene="msrB"
                     /locus_tag="PK634_14380"
                     /pseudo
     CDS             749..1174
                     /gene="msrB"
                     /locus_tag="PK634_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
     gene            1408..>2289
                     /locus_tag="PK634_14385"
     CDS             1408..>2289
                     /locus_tag="PK634_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98963.1"
                     /translation="MKHLFSWLGLSSLVGPMVCAAQVSIFRPNIGVPALPLTNGTFDI
                     EIQAAPGLASNGWSVLLLNDLRNWTCAVERVDYGFVVYGNTATGYLLTARAPASAPEE
                     VFKLRVGHSVAGGATNRHCVSILSNYETNFYILHYADPQVETTNATAANGAGGSHGSV
                     QSVQWAAGAFSLIHPRFMFNTGDEVENGVASFYPRYLDAVDAIGAPLLITRGNNDSQG
                     SFSNWKRDLGQSTYTLALGSFYVAMCDYSTNELRSWFTNDYARSFTNAAIRYRLFGQH
                     FNSGMSAHAPAAGQYPDL"
BASE COUNT          389 a          793 c          729 g          378 t
ORIGIN      
        1 gagaacgagc cgcgcgacaa ggtgcccaac ggcgtcatca gccgctggcc gatcaccgcc
       61 tcgggcgagt ggacggatcg cgtggtgcag aaccgcgatt tcgtgtgggc cacgatcgag
      121 ctccccgggc cgcgcgacct ccacgtggtc agcgtgcatc tcaagtccgg cgaaagctgg
      181 taccagaaga agggccggca gcgcgaggcc gtcgccctgc tggactacat ccggcgcgcc
      241 ggctggccgg ccgacgatta cctcgtcatc gcgggcgacc tgaacacggt gagccgtaac
      301 gagcccctgt tcgatatctg ggcgtccctg gtcagcgacg cccggcagcc cgcggaccag
      361 cagggggaca aggacaccaa catcccgcgc cagcagtgct acgattatgt cctgcccggt
      421 cccgcgctgg acgccctgca cgttcccgtc gaggccggcg gcctgacgtt cccggacggc
      481 ctggtcttcg acagccgcct ctggtccccg ccgcccgcgc cgatccggcc gggggattcc
      541 gccgaggaag acctgcagca cctcccggtc atgaaagcct tccgcctgcc gtaacctggc
      601 cgggagttga attcgcgttc cgccggtgta ccttgcccat ggcatgggat cgctcatgaa
      661 aagaccgccg ttgatcctgc tgatggcgtt cacgctcctc gccgccggga cggcgcgggg
      721 cgcggcgcgg gagtccacga ccgtgaaaaa ccgggacgcc ttgaagacga aactcacgcc
      781 cctgcagttc caggtgaccc agcagtgcgg caccgagccg ccgttccgca acgcgtactg
      841 ggacaaccac cgcgagggga tttacgtgga cgtggtctcc ggcgagccgt tgttcagttc
      901 gacggacaag ttcgattccg gcaccggctg gccgagcttc accaagccga tcgaggcgac
      961 gaacattgtg aaaaaggccg accgcagcca cttcatggac cgggtcgaag tgcgcagcaa
     1021 gggcggtgat tcccaccttg gccacgtctt cgacgacggc ccgcggccct ccggccttcg
     1081 ccactgcatt aacagcgcct cgctccggtt catcccgaag gaagacctgg agaaggaagg
     1141 ctacggggcg taccggaagc tgttcgagat gtagggtttc tgggggaagg ccatcggccg
     1201 cttctgccgg gcgcgcaccc ggcggacaac acgggctcgg gcgggggccg ccgcgtccgc
     1261 gccgatgccc gcgggaagaa aacacccgcc tgcggcgaca ggtattctcg tgacaacccc
     1321 tttctccgga tgcgcggcag ccgggcggtg tttacacctg gggcgtttcc ctgtatacct
     1381 atacgtataa gtcgagggcc cttttgaatg aagcacttgt tttcctggct ggggttgtcg
     1441 agcctcgtcg ggccgatggt atgcgcggca caagtgtcca ttttccggcc caatataggc
     1501 gtaccggcgc tgccgttgac caatgggacc ttcgacatcg agatccaggc cgcgccgggc
     1561 ctggcctcca acggatggtc cgtgctcctc ctcaacgact tgcggaattg gacctgcgcc
     1621 gtcgagcgcg tggactacgg cttcgtggtc tacgggaaca cggccacggg ctacctgctg
     1681 accgcgcgcg ccccggcctc ggcgcccgag gaggtcttta agctccgggt cggccacagc
     1741 gtcgcgggcg gcgcgaccaa ccggcactgc gtgagtatcc tgtcgaacta cgagaccaac
     1801 ttctacatcc tccactacgc cgaccctcag gtggagacca ccaacgccac ggcggccaac
     1861 ggcgccggtg gctcgcacgg cagcgtgcag tccgtccagt gggcggccgg cgccttcagc
     1921 ctgatccacc cgcgcttcat gttcaacacg ggcgacgagg tggagaacgg cgtcgcgagt
     1981 ttctaccccc gctacctgga cgccgtggac gccatcggcg cccccctgct gatcacgcgc
     2041 ggcaacaacg actcgcaggg ctcgttctcc aactggaagc gcgacctcgg gcagtccacc
     2101 tacacccttg ccttgggctc gttctacgtg gcgatgtgcg attactcgac gaacgagctc
     2161 cggtcatggt tcacgaacga ctacgcccgc tccttcacga acgccgccat ccggtaccgt
     2221 ctgttcggtc agcacttcaa cagcggcatg tcggcccacg cgccggcggc gggccagtat
     2281 cccgatctc
//
LOCUS       DAOOTK010000214         2106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_2495
            NODE_38419_length_2106_cov_3.581180, whole genome shotgun sequence.
ACCESSION   DAOOTK010000214 DAOOTK010000000
VERSION     DAOOTK010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864570
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:30:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,880
            CDSs (total)                      :: 2,840
            Genes (coding)                    :: 2,821
            CDSs (with protein)               :: 2,821
            Genes (RNA)                       :: 40
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38419_length_2106_cov_3.581180"
                     /isolate="AMR_MDS_2495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK634_14390"
     CDS             <1..246
                     /locus_tag="PK634_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98964.1"
                     /translation="LPAPVPVEVRGEGVIELMVDGWRETLPYRGWYFQGTELSLGAIS
                     NQPLSFTVNGNLVTGGTWRAAVTGAMTLQASQIRPAE"
     gene            complement(225..1307)
                     /locus_tag="PK634_14395"
     CDS             complement(225..1307)
                     /locus_tag="PK634_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOW98965.1"
                     /translation="MAWTFATAFGGHILGFFRLGSKQPPSPYITPPDSVLAKGEVGYD
                     GQMFLTVALDPLLANPGTRAALDNPRYRYRRILFPVLGYALSLGQRALIPWMLVLING
                     VCFVSVTMVTARLLESLGQPARAALFALGIPGFWCALLLTTSELLAGLLFALALFCYG
                     RERHGWTAVFFGLAGLTHETMLIVIGTLAFPLAGQRKWRAAGILALGSLPAVLWNLFI
                     LWKLPPAGSTTGLVASFSWPGAGIAQKVLTIARGPFSAKGLFDSATFLLLAVTFGLLV
                     IAFLQERRSRFILPCALAYLGFFTLSKMHLIAYYIDFLRVFSNAVFLLIPALAWGAGR
                     RATSTLLLAWSACSAALVAAYSAGLI"
     gene            1496..>2106
                     /gene="tyrS"
                     /locus_tag="PK634_14400"
     CDS             1496..>2106
                     /gene="tyrS"
                     /locus_tag="PK634_14400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOW98966.1"
                     /translation="MNTSAHAEFDVLASRSVDLHSADELRARLESAKKENRPLRVKYG
                     ADPSAPDIHLGHVVGLNKLREFQDLGHTVVFIIGDFTGMIGDPSGKSQTRKALSREQV
                     QANAKTYQEQVFKILDPARTETRFNSEWLSPMSFEDVIRLSAHVTVAQMLARDDFGKR
                     YAAQQPISLVEFLYPLVQAYDSVMVRADVELGGTDQLFNLLLGR"
BASE COUNT          424 a          647 c          708 g          327 t
ORIGIN      
        1 ctgccggcgc ccgtccccgt ggaggtgcgc ggtgagggcg tgatcgaact gatggtcgac
       61 ggctggcgcg agaccctgcc gtacaggggc tggtatttcc aagggaccga gctgtcgctc
      121 ggcgccatat cgaaccagcc tctttccttc accgtgaatg gaaacctggt gacgggcggc
      181 acatggcggg cggcggtgac cggggcgatg acgctgcagg cgagtcagat caggcccgct
      241 gaataagccg cgaccagcgc ggcgctgcac gcgctccagg ccagaaggag tgtgctcgtg
      301 gcccgccgcc cggcgcccca ggcgagcgcc gggatgagca ggaagaccgc gttactgaaa
      361 acgcgcagga aatcgatgta ataggcgatc aggtgcatct tcgacagggt aaaaaagccg
      421 aggtaggcga gcgcgcacgg caggatgaag cgcgatcgcc gttcctggag gaaggcgatc
      481 acgagcaggc cgaacgtgac ggcaaggaga agaaaagttg cgctgtcgaa gagccccttg
      541 gcgctgaaag ggcctcgtgc gatggtgagg accttttgcg cgattcccgc gcctggccag
      601 gagaaactcg cgaccaggcc ggtggtgctc ccggccggcg gcagcttcca tagaatgaac
      661 aggttccaca acacagccgg caacgaaccc agtgcgagta ttcctgccgc cctccatttc
      721 cgttgcccgg cgagggggaa ggccagtgtc ccgatcacga tcagcattgt ttcgtgggtg
      781 agcccggcaa gtccgaagaa gacagccgtc catccgtgcc gctcgcggcc gtagcagaag
      841 agcgccaggg caaacagcag gccggcgagc agttcggagg tggtcaggag cagggcgcac
      901 cagaaacccg ggatacccag cgcgaagagc gcggcccggg ccggttgtcc gagtgattcc
      961 agcaggcgcg ccgtcaccat ggtgacggac acaaaacaaa cgccgttgat caacaccagc
     1021 atccacggga taagggcgcg ttgccccagg gaaagggcat agcccagcac ggggaacaga
     1081 atgcggcggt atcgataccg gggattgtcc agggcggcgc gggttccggg gttggccagc
     1141 aaaggatcca gggctaccgt caggaacatc tgcccgtcat agccgacctc gcccttggcc
     1201 aggacggagt ccgggggggt gatataggga gaaggcggct gtttcgaccc gagccggaag
     1261 aagcccagta tgtggccgcc gaatgcggtc gcaaaggtcc aagccatgat cgccgcgaca
     1321 accgccgcgg gaattgcggt gaagccgaca cgagccgcgc gcgtcggact ggggcaacgg
     1381 tcgaagtccg tcatggaacg gaactatagc cgcagacgga tggctttgtc cacgcccggc
     1441 ctttttgtct tcgttttcac ttctcaatcc gctatcattt aaaagaatac ggaccatgaa
     1501 tacatctgcc catgccgagt ttgacgtcct ggcctcccgt tccgtggatc tccattcggc
     1561 ggacgaattg cgggcccgcc tggagtccgc caagaaggag aaccgcccgc tgcgggtgaa
     1621 gtacggggcg gacccgtccg cgccggacat ccacctcggc cacgtggtcg gcctgaacaa
     1681 gctccgggag ttccaggacc tcgggcatac cgtcgtgttc atcatcggcg atttcacggg
     1741 tatgatcggc gacccgtccg gcaagtcgca gacccgcaag gccctgtcgc gcgagcaggt
     1801 gcaagccaac gcgaagacct accaggagca ggtcttcaag attctcgacc cggcgcgcac
     1861 ggagacgcgg ttcaactccg agtggctgtc cccgatgagc ttcgaggacg tcatccgcct
     1921 ctccgcgcac gtgaccgtcg cccagatgct ggcccgggac gatttcggca agcggtacgc
     1981 cgcgcagcag ccgatctcgc tggtggagtt cctgtacccg ctcgtccagg cctacgactc
     2041 cgtgatggtc cgggccgacg tcgaactggg cggaacggac cagttgttca acctgctgct
     2101 cggccg
//
